Determination of N,N'-ethylenebisstearamide additive in polymer by normal phase liquid chromatography with evaporative light scattering detection.
A new method for N,N'-ethylenebisstearamide (EBS) analysis was developed and validated in normal phase-HP liquid chromatography (NP-HPLC) with diol column at 50 degrees C with 100% CHCl(3) at 1 mL min(-1) and evaporative light scattering detection with elution time at 3.0 min. EBS solubility was the best at 0.80 gL(-1) in CHCl(3)/methanol 90:10. The molecular structure of commercial samples of EBS was determined by GC-MS which ascertained that the main structure is C18/C18 at approximately 45%. The remaining part was constituted by molecules with different alkyl chain length. The HPLC quantification method was proved linear (r=0.9983), accurate (99.6%) and precise (1.95%). Limit of quantification (LOQ) and limit of detection (LOD) were equal to 2.0 and 0.8 microg mL(-1), respectively. The suitability of this method was assessed with a dissolution/precipitation extraction procedure of EBS from ethylene vinyl acetate (EVA) polymer which showed that other additives and polymer do not interfere with EBS analysis. The intra-day and day-to-day precisions of extraction method were equal to 9.1% and 9.9%, respectively.
Gaudin
Karen
K
Laboratoire de Chimie Analytique, PPF - Médicament-Parasitologie, UFR Pharmacie, Université Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux cedex, France.
Ho-Sung
Helen
H
Bleton
Jean
J
Joseph-Charles
Julienne
J
Dallet
Philippe
P
Puig
Pascal
P
Dubost
Jean-Pierre
JP
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005029:Ethylenediamines / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D011108:Polymers / Q000032:analysis; D011145:Polyvinyls / Q000032:analysis*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013386:Succinates / Q000032:analysis*; D014835:Volatilization
D005029:Ethylenediamines; D011108:Polymers; D011145:Polyvinyls; D013386:Succinates; C041447:N,N'-ethylenediamine disuccinic acid; C016438:ethylenevinylacetate copolymer