Determination of atenolol by the micelle-stabilized room-temperature phosphorescence methodology.
A micellar-stabilized room-temperature phosphorescence (MS-RTP) method for the determination of atenolol has been developed in micellar solutions of sodium dodecylsulphate (SDS) in the presence of thallium(I) as a heavy atom and sodium sulphite as an oxygen scavenger. The effects of thallium(I) nitrate, SDS and sodium sulphite concentrations on atenolol MS-RTP intensity were studied. Optimized conditions to obtain maximum sensitivity were 0.015 mol/L thallium(I) nitrate, 0.1 mol/L SDS and 0.0075 mol/L sodium sulphite. The maximum phosphorescence signal was completely developed in 10 min and the intensity was measured at lambda(ex) = 272 nm and lambda(em) = 412 nm. The linear range of application obtained was 2.01-16.00 microg/mL. The detection limit estimated from the least-squares regression analysis was 0.86 microg/mL and the relative standard deviation of 10 replicates was 1.7%. The proposed method was applied to the determination of atenolol in a pharmaceutical formulation. The quantitation was carried out by means of standard calibration, standard-additions calibration and Youden calibration. These three experiments were necessary to evaluate the presence of constant and proportional errors due to the matrix.
Castillo
Marcela A
MA
División Química Analítica, Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 esq. 115, 1900 La Plata, Argentina.
Gambino
Luisina Ongaro
LO
Polizzi
Germán
G
Andreetta
Héctor A
HA
Bruzzone
Liliana
L
D001262:Atenolol / Q000032:analysis*; D002138:Calibration; D016018:Least-Squares Analysis; D008163:Luminescent Measurements / Q000379:methods*; D008823:Micelles; D004364:Pharmaceutical Preparations / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012996:Solutions / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D014665:Vasodilator Agents / Q000032:analysis*
D008823:Micelles; D004364:Pharmaceutical Preparations; D012996:Solutions; D014665:Vasodilator Agents; D012967:Sodium Dodecyl Sulfate; D001262:Atenolol