title: Utility of mycobacterial interspersed repetitive unit typing for differentiating Mycobacterium tuberculosis isolates in Wuhan, China.
abstract: Mycobacterial interspersed repetitive unit (MIRU) typing has been found to allow rapid, reliable, high-throughput genotyping of Mycobacterium tuberculosis, and may represent a feasible approach to study M. tuberculosis molecular epidemiology. To evaluate the use of MIRU typing in discriminating M. tuberculosis strains, isolates from 105 patients in Wuhan City, China, were genotyped by this method as compared to spoligotyping. MIRU typing identified 55 types that defined 21 clusters and 34 unique isolates. The discriminatory power was high [Hunter-Gaston discriminatory index (HGDI), 0.97]. Spoligotyping showed that 86 (81.9 %) of 105 isolates belonged to the Beijing family genotype. For Beijing family and non-Beijing strains, the discriminatory power of MIRU was high (HGDI, 0.95 and 0.98, respectively). Among the alleles of the MIRU loci for the Beijing family, only locus 26 was highly discriminative, but for non-Beijing strains, loci 10, 16 and 26 were highly discriminative. MIRU typing is a simple and fast method which may be used for preliminary screening of M. tuberculosis isolates in China.
authors.affiliation: National Center for Women and Children's Health, China CDC, Beijing 100013, China.|Department of Epidemiology, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Han
authors.affiliation: Department of Social Medicine, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Wuhan Tuberculosis Prevention and Treatment Hospital, Wuhan 430030, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Wuhan Tuberculosis Prevention and Treatment Hospital, Wuhan 430030, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ren
authors.affiliation: National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Chao
authors.affiliation: National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Aizhen
authors.initials: A
authors.lastname: Guo
authors.affiliation: Department of Epidemiology, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Lingxiang
authors.initials: L
authors.lastname: Ye
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D006801:Humans; D020071:Interspersed Repetitive Sequences / Q000235:genetics; D017720:Molecular Epidemiology / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial