title: Purification, crystallization and preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum JCM1217.
abstract: A recombinant galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) from Bifidobacterium longum JCM1217 has been prepared and crystallized by the hanging-drop vapour-diffusion method using 10 mg ml(-1) purified enzyme, 0.01 M zinc sulfate, 0.1 M MES buffer pH 5.9-6.4 and 20-22%(v/v) PEG MME 550 in the presence of 5 mM disaccharide ligands. Suitable crystals grew after 10 d incubation at 293 K. The crystals belong to space group C222(1), with unit-cell parameters a = 106.3, b = 143.6, c = 114.6 A for the lacto-N-biose I complex and a = 106.4, b = 143.4, c = 115.5 A for the galacto-N-biose complex, and diffracted to 1.85 and 1.99 A resolution, respectively.
authors.affiliation: Graduate School of Biostudies, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Wada
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Suzuki
authors.forename: Shinya
authors.initials: S
authors.lastname: Fushinobu
authors.forename: Motomitsu
authors.initials: M
authors.lastname: Kitaoka
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Wakagi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Shoun
authors.forename: Hisashi
authors.initials: H
authors.lastname: Ashida
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Kumagai
authors.forename: Takane
authors.initials: T
authors.lastname: Katayama
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D001644:Bifidobacterium / Q000737:chemistry*; D003460:Crystallization; D017931:DNA Primers; D010957:Plasmids; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D014961:X-Ray Diffraction
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D017931:DNA Primers; D011994:Recombinant Proteins