title: Construction of p16Slux, a novel vector for improved bioluminescent labeling of gram-negative bacteria.
abstract: A novel vector has been constructed for the constitutive luminescent tagging of gram-negative bacteria by site-specific integration into the 16S locus of the bacterial chromosome. A number of gram-negative pathogens were successfully tagged using this vector, and the system was validated during murine infections of living animals.
authors.affiliation: Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland.
authors.forename: Christian U
authors.initials: CU
authors.lastname: Riedel
authors.forename: Pat G
authors.initials: PG
authors.lastname: Casey
authors.forename: Heidi
authors.initials: H
authors.lastname: Mulcahy
authors.forename: Fergal
authors.initials: F
authors.lastname: O'Gara
authors.forename: Cormac G M
authors.initials: CG
authors.lastname: Gahan
authors.forename: Colin
authors.initials: C
authors.lastname: Hill
mesh_terms: D000818:Animals; D002876:Chromosomes, Bacterial; D005822:Genetic Vectors; D006090:Gram-Negative Bacteria / Q000235:genetics* / Q000302:isolation & purification; D008164:Luminescent Proteins / Q000378:metabolism*; D051379:Mice; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D011994:Recombinant Proteins
chemical_list: D008164:Luminescent Proteins; D012336:RNA, Ribosomal, 16S; D011994:Recombinant Proteins