[Temporal expression of HCMV IE1 and pp65 in human glioma U(251) cells].
OBJECTIVE
To determine whether U(251) cells are permissive for human cytomegalovirus (HCMV), and to investigate the characteristics of temporal expression of proteins IE1 and pp65.


METHODS
U(251) cells were infected with HCMV, and then the cells were observed under the transmission electronic microscope, and the viral nucleic acid was detected by PCR, and the expression levels of IE1 and pp65 were analyzed by immunohistochemical assay with anti-IE1 monoclonal antibody and anti-pp65 monoclonal antibody at various time spost infection.


RESULTS
Morphological changes of the infected cells appeared under the transmission electron microscope. The viral nucleic acid was detected successfully by PCR. The expression of IE1 was detected firstly at 4h post infection, and reached a peak within 14h, and then decreased. The incoming pp65 was detected at 1h, the low expression levels of pp65 were detected firstly at 4h, and they could remain relatively constant through 96 h, but the maximum expression occurred at 120 h.


CONCLUSIONS
Human glioma U(251) cells are permissive for HCMV, the temporal cascade of HCMV gene expression can be observed in the infected U(251) cells, but it is delayed obviously in the human fibroblast.
Li
Jian-Hua
JH
Department of Microbiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Fu
Ying
Y
Chen
Li-Yu
LY
Dai
Gan
G
Luo
Min-Hua
MH
Yang
Tao
T
D045744:Cell Line, Tumor; D003587:Cytomegalovirus; D003586:Cytomegalovirus Infections; D005910:Glioma / Q000378:metabolism* / Q000821:virology; D006801:Humans; D017874:Immediate-Early Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D014763:Viral Matrix Proteins / Q000378:metabolism*
C088645:IE1 protein, cytomegalovirus; D017874:Immediate-Early Proteins; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa