High throughput liquid chromatography with sub-2 microm particles at high pressure and high temperature.
In this study, ultra performance liquid chromatography (UPLC) using pressures up to 1,000 bar and columns packed with sub-2 microm particles has been combined with high temperature mobile phase conditions (up to 90 degrees C). By using high temperature ultra performance liquid chromatography (HT-UPLC), it is possible to drastically decrease the analysis time without loss in efficiency. The stability and chromatographic behavior of sub-2 microm particles were evaluated at high temperature and high pressure. The chromatographic support remained stable after 500 injections (equivalent to 7,500 column volumes) and plate height curves demonstrated the capability of HT-UPLC to obtain fast separations. For example, a separation of nine doping agents was performed in less than 1 min with sub-2 microm particles at 90 degrees C. Furthermore, a shorter column (30 mm length) was used and allowed a separation of eight pharmaceutical compounds in only 40s.
Nguyen
Dao T-T
DT
Laboratory of Analytical Pharmaceutical Chemistry, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland.
Guillarme
Davy
D
Heinisch
Sabine
S
Barrioulet
Marie-Pierre
MP
Rocca
Jean-Louis
JL
Rudaz
Serge
S
Veuthey
Jean-Luc
JL
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D006358:Hot Temperature; D007700:Kinetics; D008956:Models, Chemical; D010316:Particle Size; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification*; D016062:Porosity; D011312:Pressure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013696:Temperature; D013997:Time Factors
D004364:Pharmaceutical Preparations