[Effect of gene polymorphism of TNF-beta on the concentration of TNF in serum of patient with endometriosis].
OBJECTIVE
To determine the polymorphism in +252 site of tumor necrosis factor-beta(TNF-beta) gene in patients with or without endometriosis, to evaluate the levels of TNF-alpha and TNF-beta in the serum with or without endometriosis, to explore the relation between polymorphism of TNF-beta gene and the genetic susceptibility of endometriosis, and to explore the pathogenic mechanism of endometriosis at gene level.


METHODS
By polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, polymorphism on +252 site of TNF-beta gene was measured in 82 patients with endometriosis (the endometriosis group) and 80 patients without endometriosis (the control group). With the sandwich-enzyme-linked immunosorbent assay (ELISA), the levels of TNF-alpha and TNF-beta in the serum of the two groups were determined.


RESULTS
The TNF-beta level in the serum in the endometriosis group with TNF-beta gene +252 site AA genotype significantly increased, compared with GG genotype (t=2.029, P<0.05); while TNF-alpha and TNF-beta level in the serum had no statistical significance in patients with other genotypes in TNF-beta gene +252 site in the endometriosis group and the control group.


CONCLUSIONS
TNF-beta gene +252 site AA genotype might be enhance TNF-beta level in the serum of patients with endometriosis.
Luo
Min
M
Department of Obstetrics and Gynecology, General Hospital of Guangzhou Military Area Command, Guangzhou 510010, China.
Shen
Dong-Xiang
DX
Zhang
Hong-Bin
HB
Wang
Jie
J
Zong
Li-Li
LL
Guan
Ting
T
He
Yuan-Li
YL
D000293:Adolescent; D000328:Adult; D004715:Endometriosis / Q000097:blood* / Q000235:genetics; D005260:Female; D006801:Humans; D008233:Lymphotoxin-alpha / Q000097:blood* / Q000235:genetics*; D011110:Polymorphism, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*; D055815:Young Adult
D008233:Lymphotoxin-alpha; D014409:Tumor Necrosis Factor-alpha