Base changes in the fliC gene of Edwardsiella tarda: possible effects on flagellation and motility.
Edwardsiella tarda is a broad host-range pathogen infecting both animals and humans. E. tarda isolates from red sea bream Pagrus major are non-motile, whereas isolates from Japanese eel Anguilla japonica and Japanese flounder Paralichthys olivaceus are motile with peritrichous flagella. We compared the fliC gene coding for flagellin (FliC) in motile and non-motile E. tarda strains isolated from diseased fish. Twenty-two amino acid residues differed in the predicted FliC amino acid sequences between non-motile and motile strains. There were no significant differences either in the upstream sequences regulating transcription of the fliC gene or in the fliC transcript levels between motile and non-motile strains. The predicted secondary structure of FliC in non-motile E. tarda differed from that of motile strains, and the modeled data suggested that the secondary structure may be the important factor responsible for non-flagellation in the non-motile strains.
Okuda
Jun
J
Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8528, Japan. okudaj@hiroshima-u.ac.jp
Murayama
Fumiyasu
F
Yamanoi
Eisuke
E
Iwamoto
Emi
E
Matsuoka
Satoru
S
Nishibuchi
Mitsuaki
M
Nakai
Toshihiro
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D050260:Carbohydrate Metabolism / Q000502:physiology; D020609:Edwardsiella tarda / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D004756:Enterobacteriaceae Infections / Q000382:microbiology / Q000662:veterinary; D004926:Escherichia coli / Q000235:genetics; D005393:Fish Diseases / Q000382:microbiology; D005407:Flagella / Q000235:genetics*; D005408:Flagellin / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D005432:Flounder / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009068:Movement / Q000502:physiology*; D016366:Open Reading Frames / Q000235:genetics; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D021541:Sea Bream / Q000382:microbiology; D016415:Sequence Alignment; D001616:beta-Galactosidase / Q000032:analysis / Q000378:metabolism
D011994:Recombinant Proteins; D005408:Flagellin; D001616:beta-Galactosidase