[Effect of EphA2 protein on the expression of VEGF and MMP9 proteins in HCT116 cells].
OBJECTIVE
To determine the effect of EphA2 protein on the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase 9 (MMP9) proteins in HCT116 cells.


METHODS
High expression of EphA2 protein in HCT116 cells was confirmed by Western blot. HCT116 cells were transfected with EphA2 antisense oligonucleotide. The expression of the transfection efficiency was analyzed by Western blot. VEGF proteins in the cell supernatants were detected by enzyme linked immunosorbent assay(ELISA), and the expressions of MMP9 in cell supernatants were examined by gelatin zymography.


RESULTS
EphA2 antisense oligonucleotide suppressed the expression of VEGF and MMP9 proteins in HCT116 cells.


CONCLUSIONS
EphA2 could decrease the invasion and metastasis of HCT116 cells by suppressing the expression of VEGF and MMP9.
Jiang
Ping
P
Department of Pathology, College of Basic Medicine, Central South University, Changsha 410078, China.
Li
Jing-He
JH
Luo
Geng-Qiu
GQ
Liu
Jie
J
D045325:HCT116 Cells; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D016376:Oligonucleotides, Antisense; D036104:Receptor, EphA2 / Q000235:genetics / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D016376:Oligonucleotides, Antisense; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D036104:Receptor, EphA2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9