Dysregulation of CD47 and the ligands thrombospondin 1 and 2 in multiple myeloma.
CD47 and thrombospondin 1 and 2 (TSP1 and TSP2) expression were analysed by real-time reverse transcription polymerase chain reaction in fluorescence-activated cell sorted plasma cells (PCs) from patients at consecutive stages of multiple myeloma (MM) development. 80% of MM patients, but only 39% of patients with monoclonal gammopathy of undetermined significance (MGUS) expressed CD47; median expression level increased 10-fold with progression from MGUS to MM. Elevated TSP1/TSP2 levels occurred in bone marrow cultures from MM patients compared with healthy donors. CD47 and TSP1/TSP2 may have a potential role in the pathophysiology of MM, probably in the interaction between MM PCs and the microenvironment.
Rendtlew Danielsen
Jannie M
JM
Department of Haematology 54P4, Herlev University Hospital, University of Copenhagen, Denmark. jrd@cancer.dk
Knudsen
Lene Meldgaard
LM
Dahl
Inger Marie
IM
Lodahl
Marianne
M
Rasmussen
Thomas
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001856:Bone Marrow Examination; D051928:CD47 Antigen / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016130:Immunophenotyping; D008875:Middle Aged; D009101:Multiple Myeloma / Q000276:immunology* / Q000401:mortality; D010265:Paraproteinemias / Q000276:immunology; D010950:Plasma Cells / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D016019:Survival Analysis; D019700:Thrombospondin 1 / Q000235:genetics*; D019699:Thrombospondins / Q000235:genetics*; D000099262:Thrombospondin 2
D051928:CD47 Antigen; D019700:Thrombospondin 1; D019699:Thrombospondins; D000099262:Thrombospondin 2