Natronococcus jeotgali sp. nov., a halophilic archaeon isolated from shrimp jeotgal, a traditional fermented seafood from Korea.
A novel halophilic archaeon (strain B1(T)) belonging to the genus Natronococcus was isolated from shrimp jeotgal, a traditional fermented food from Korea. Colonies of this strain were orange-red and cells were non-motile cocci that stained Gram-variable. Strain B1(T) grew in 7.5-30.0 % (w/v) NaCl and at 21-50 degrees C and pH 7.0-9.5, with optimal growth occurring in 23-25 % (w/v) NaCl and at 37-45 degrees C and pH 7.5. Strain B1(T) was most closely related to the type strain of Natronococcus occultus, with which it shared 97.91 % 16S rRNA gene sequence similarity. Within the phylogenetic tree, this novel strain shared a branching point with N. occultus and occupied a phylogenetic position that was distinct from the main Natronococcus branch. The degree of DNA-DNA hybridization with the type strain of N. occultus, the most closely related species phylogenetically, was 16.4 %. On the basis of these results, it is concluded that strain B1(T) represents a novel species of the genus Natronococcus, for which the name Natronococcus jeotgali is proposed. The type strain is B1(T) (=KCTC 4018(T)=DSM 18795(T)=JCM 14583(T)=CECT 7216(T)).
Roh
Seong Woon
SW
Biological Resource Center, KRIBB, Daejeon 305-806, Korea.|University of Science & Technology, 52 Eoeun-dong, Daejeon 305-333, Korea.
Nam
Young-Do
YD
Biological Resource Center, KRIBB, Daejeon 305-806, Korea.|University of Science & Technology, 52 Eoeun-dong, Daejeon 305-333, Korea.
Chang
Ho-Won
HW
Biological Resource Center, KRIBB, Daejeon 305-806, Korea.
Sung
Youlboong
Y
Biological Resource Center, KRIBB, Daejeon 305-806, Korea.
Kim
Kyoung-Ho
KH
Biological Resource Center, KRIBB, Daejeon 305-806, Korea.
Lee
Ho-Jae
HJ
Biological Resource Center, KRIBB, Daejeon 305-806, Korea.
Oh
Hee-Mock
HM
Biological Resource Center, KRIBB, Daejeon 305-806, Korea.
Bae
Jin-Woo
JW
Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Korea.|Biological Resource Center, KRIBB, Daejeon 305-806, Korea.|University of Science & Technology, 52 Eoeun-dong, Daejeon 305-333, Korea.
D000818:Animals; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008969:Molecular Sequence Data; D019634:Natronococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D009693:Nucleic Acid Hybridization; D033542:Pandalidae / Q000382:microbiology*; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012758:Shellfish / Q000382:microbiology*; D013696:Temperature
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D010860:Pigments, Biological; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S