title: Inactivation of thiolase by 2-alkynoyl-CoA via its intrinsic isomerase activity.
abstract: Selective inactivation of cytosolic thiolase by 2-alkynoyl-CoA via its intrinsic isomerase activity was studied, which provides an example for rationally developing mechanism-based inhibitors based on a side activity of the enzyme, and may become a supplemental method for better treatment of cardiovascular disease and cancer.
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, PRC.
authors.forename: Long
authors.initials: L
authors.lastname: Wu
authors.forename: Jia
authors.initials: J
authors.lastname: Zeng
authors.forename: Guisheng
authors.initials: G
authors.lastname: Deng
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Liu
authors.forename: Xiusheng
authors.initials: X
authors.lastname: Chu
authors.forename: Ding
authors.initials: D
authors.lastname: Li
mesh_terms: D000105:Acetyl Coenzyme A / Q000737:chemistry* / Q000378:metabolism*; D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002384:Catalysis; D002460:Cell Line; D004789:Enzyme Activation; D007536:Isomerism; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D015394:Molecular Structure; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D013438:Sulfhydryl Compounds; D000105:Acetyl Coenzyme A