title: Behavior of CD34+ cells isolated from patients with polycythemia vera in NOD/SCID mice.
abstract: OBJECTIVE
  We investigated if polycythemia vera (PV) peripheral blood (PB) CD34+ cells contain cells capable of engrafting nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice and if the JAK2V617F mutational burden of these cells alters their behavior in NOD/SCID mice.
  
  
  METHODS
  CD34+ cells isolated from patients with PV, idiopathic myelofibrosis (IM), or granulocyte colony-stimulating factor-mobilized normal donors were transplanted into sublethally irradiated NOD/SCID mice. Cells engrafted into the NOD/SCID mice were analyzed flow cytometrically using lineage-specific antibodies. Genomic DNA was extracted from granulocytes, CD34+ cells, and sorted human CD45(+) cells purified from the bone marrow cells of these mice to examine their JAK2V617F mutational burdens.
  
  
  RESULTS
  Multilineage human cell engraftment was observed in mice transplanted with CD34+ cells from mobilized normal volunteers, IM patients and PV patients with high JAK2V617F burden, but not in mice receiving grafts from PV patients with low JAK2V617F burden. The differentiation program of engrafting PV CD34+ cells with high JAK2V617F burden was remarkably different than that of IM CD34+ cells. The JAK2V617F allele frequency in the human CD45+ cells isolated from the mice receiving CD34+ cells was lower than that observed in the CD34+ cell grafts, indicating the persistence of a JAK2V617F negative compartment of stem cells.
  
  
  CONCLUSIONS
  We conclude that PB CD34+ cells from PV patients with high JAK2V617F burden and patients with IM contain NOD/SCID repopulating cells, and that differentiation program of IM and PV CD34+ cells are dramatically different.
authors.affiliation: Division of Hematology/Oncology, Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.
authors.forename: Takefumi
authors.initials: T
authors.lastname: Ishii
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Selcuk
authors.initials: S
authors.lastname: Sozer
authors.forename: Jun
authors.initials: J
authors.lastname: Shi
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Ronald
authors.initials: R
authors.lastname: Hoffman
authors.forename: Mingjiang
authors.initials: M
authors.lastname: Xu
mesh_terms: D000818:Animals; D018952:Antigens, CD34; D016022:Case-Control Studies; D019070:Cell Lineage; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D020125:Mutation, Missense / Q000502:physiology*; D011087:Polycythemia Vera / Q000473:pathology*; D055728:Primary Myelofibrosis / Q000473:pathology; D014183:Transplantation, Heterologous
chemical_list: D018952:Antigens, CD34; C507924:JAK2 protein, human; D053614:Janus Kinase 2