Automated CD14+ monocyte collection with the autoMNC program of the COM.TEC cell separator.
BACKGROUND
The standard mononuclear cell (MNC) program of the COM.TEC device (Fresenius HemoCare GmbH) showed excellent collection efficiency of CD14+ monocytes. A major disadvantage was high content of residual cells in MNC harvests, which could influence dendritic cell (DC) culture.


METHODS
The autoMNC program (COM.TEC) was compared with the standard MNC program (n = 12). Additionally, two cycle volumes (300 mL vs. 450 mL, n = 19) were compared (standard MNC program). Samples were assayed for white blood cells (WBCs), red blood cells (RBCs), granulocytes (PMNs), hematocrit, and platelets (PLTs) on an automated blood cell counter (Sysmex K 4500, TAO Medical). CD14+ cells were analyzed by flow cytometry (FACSCalibur, BD).


RESULTS
The autoMNC program produced 1.33 x 10(9) +/- 0.36 x 10(9) CD14+ cells, 5.60 x 10(11) +/- 0.97 x 10(11) PLTs, and 1.43 x 10(11) +/- 0.37 x 10(11) RBCs. Compared to the standard MNC program, significantly higher PLT yields but lower RBC yields and product volume were harvested. Increasing the CV from 300 to 450 mL dropped the product volume, residual PLTs, and RBCs significantly, whereas WBC and monocyte yields did not change. The WBC predonation counts of donors correlated significantly with monocyte yields.


CONCLUSIONS
The autoMNC program reduced the buffy coat (BC) volume and RBC yields in products compared to the standard MNC program. Increasing the CV (standard MNC program) reduced residual PLTs, RBCs, and the BC volume of MNC harvests. The donor WBC predonation count was a good predictor for the monocyte yield of products.
Strasser
Erwin F
EF
Transfusion Medicine and Hemostasis Department, University Hospital Erlangen, Erlangen, Germany. erwin.strasser@trans.imed.uni-erlangen.de
Schremmer
Melanie
M
Hendelmeier
Martin
M
Weiss
Dominik
D
Ringwald
Jürgen
J
Zimmermann
Robert
R
Weisbach
Volker
V
Zingsem
Jürgen
J
Eckstein
Reinhold
R
D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation / Q000379:methods*; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000032:analysis*; D015203:Reproducibility of Results; D012984:Software
D018950:Lipopolysaccharide Receptors