Jannaschia donghaensis sp. nov., isolated from seawater of the East Sea, Korea.
A Gram-negative, non-motile and rod-, oval- or coccoid-shaped strain, DSW-17(T), was isolated from seawater of the East Sea, Korea, and subjected to a polyphasic taxonomic study. Strain DSW-17(T) grew optimally at pH 7.0-8.0 and 25 degrees C. It contained Q-10 as the predominant ubiquinone and C(18 : 1)omega7c as the major fatty acid. Major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid. The DNA G+C content was 65.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSW-17(T) was phylogenetically most closely affiliated to the genus Jannaschia. Strain DSW-17(T) exhibited 16S rRNA gene sequence similarity values of 96.5 % with the type strains of three recognized species of the genus Jannaschia. DNA-DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain DSW-17(T) is distinguishable from the recognized species of the genus Jannaschia. On the basis of phenotypic, phylogenetic and genetic data, strain DSW-17(T) was classified in the genus Jannaschia as a member of a novel species, for which the name Jannaschia donghaensis sp. nov. is proposed. The type strain is DSW-17(T) (=KCTC 12862(T)=JCM 14563(T)).
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Kang
So-Jung
SJ
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Park
Sooyeon
S
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Oh
Tae-Kwang
TK
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D050260:Carbohydrate Metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D006017:Glycolipids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature; D014451:Ubiquinone / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone