title: In vitro evaluation of atmospheric particulate matter and sedimentation particles using yeast bioassay system.
abstract: Little information on the evaluation of airborne particulate matter (APM) and sedimentation particles from subway stations is available. The thermal metamorphism of train wheels generating toxic particles in subway stations is a possibility. In this study, the toxicity and physiological effects of particles from subway stations were evaluated using a yeast bioassay system. Estrogenic and antiestrogenic activities of APM in APM extracts from subway stations were determined. No estrogenic activity was found in the APM fractions and their S9-activated APM samples. Sedimentation dust samples also showed no estrogen activity. In contrast, extracts from sedimentation dust samples showed antiestrogen activity. Marked yeast toxicity was observed in the samples extracted from sedimentation dust. Potent yeast toxicity was also found in the S9-activated extracts from sedimentation dust. The results suggest that sedimentation dust from a semiclosed area of a subway system has antiestrogen activity, although both the origin and generation system of this activity are uncertain. These pollutants in sedimentation dust may change to a more toxic form in vivo by S9 activation.
authors.affiliation: Graduate School of Science and Technology, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
authors.forename: Taiki
authors.initials: T
authors.lastname: Mori
authors.forename: Makiko
authors.initials: M
authors.lastname: Inudo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Takao
authors.forename: Minoru
authors.initials: M
authors.lastname: Koga
authors.forename: Takehiro
authors.initials: T
authors.lastname: Takemasa
authors.forename: Ryota
authors.initials: R
authors.lastname: Shinohara
authors.forename: Koji
authors.initials: K
authors.lastname: Arizono
mesh_terms: D000336:Aerosols / Q000378:metabolism / Q000633:toxicity; D000393:Air Pollutants / Q000378:metabolism / Q000633:toxicity; D000818:Animals; D004784:Environmental Monitoring; D020847:Estrogen Receptor Modulators / Q000009:adverse effects / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000819:agonists / Q000037:antagonists & inhibitors; D006801:Humans; D019570:Inhalation Exposure / Q000009:adverse effects*; D010316:Particle Size; D052638:Particulate Matter / Q000378:metabolism / Q000633:toxicity*; D011890:Railroads; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D018675:Toxicity Tests; D020798:Two-Hybrid System Techniques
chemical_list: D000336:Aerosols; D000393:Air Pollutants; D020847:Estrogen Receptor Modulators; D047628:Estrogen Receptor alpha; D052638:Particulate Matter