title: Repetitive DNA hypomethylation in the advanced phase of chronic myeloid leukemia.
abstract: Repetitive elements are heavily methylated in normal tissues, but hypomethylated in malignant tissues, driving the global genomic hypomethylation found in cancer. This hypomethylation results in chromosomal instability, a well-characterized feature of the advanced phases of chronic myeloid leukemia (CML). We investigated methylation changes of DNA repetitive elements (LINE1, Alu, Satellite-alpha and Satellite-2) during the progression of CML from chronic phase (CP) to blast crisis (BC). CP-CML samples were significantly more hypomethylated for all repetitive sequences compared with normal samples. Furthermore, a more profound level of hypomethylation was observed among BC samples compared with CP samples. Our data suggest that repetitive DNA hypomethylation are closely associated with CML progression.
authors.affiliation: Hematology Department, Reina Sofia Hospital, Avda. Menendez Pidal s/n, 14004 Cordoba, Spain. peperosa@teleline.es
authors.forename: Jose
authors.initials: J
authors.lastname: Roman-Gomez
authors.forename: Antonio
authors.initials: A
authors.lastname: Jimenez-Velasco
authors.forename: Xabier
authors.initials: X
authors.lastname: Agirre
authors.forename: Juan A
authors.initials: JA
authors.lastname: Castillejo
authors.forename: German
authors.initials: G
authors.lastname: Navarro
authors.forename: Edurne
authors.initials: E
authors.lastname: San Jose-Eneriz
authors.forename: Leire
authors.initials: L
authors.lastname: Garate
authors.forename: Lucia
authors.initials: L
authors.lastname: Cordeu
authors.forename: Francisco
authors.initials: F
authors.lastname: Cervantes
authors.forename: Felipe
authors.initials: F
authors.lastname: Prosper
authors.forename: Anabel
authors.initials: A
authors.lastname: Heiniger
authors.forename: Antonio
authors.initials: A
authors.lastname: Torres
mesh_terms: D020087:Alu Elements; D001752:Blast Crisis / Q000235:genetics; D019175:DNA Methylation; D004276:DNA, Satellite; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D015466:Leukemia, Myeloid, Chronic-Phase / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004276:DNA, Satellite