Determination of caderofloxacin lactate in rat plasma by high-performance liquid chromatography-mass spectrometry and its application in rat pharmacokinetic studies.
A sensitive liquid chromatography-electrospray ionization mass spectrometric (LC-ESI-MS) method for the quantification of a newly active quinolone carboxylic acid caderofloxacin lactate in rat plasma was developed and validated after precipitation method with methanol. Chromatographic separation was achieved on a reversed-phase Shimadzu 2.0 microm C18 column (150 mm x 2.00 mm) with the mobile phase of methanol-0.02% formic acid and step gradient elution resulted in a total run time of about 10.0 min. The analytes were detected by using an electrospray positive ionization mass spectrometry in the selected ion monitoring (SIM) mode. A good linear relationship was obtained in the concentration range studied (5-2000 ng/mL) (r=0.9998). The lowest limit of quantification (LLOQ) was 5 ng/mL and the lowest limit of detection (LLOD) was 2 ng/mL. Average recoveries ranged from 88.80 to 93.05% in plasma at the concentrations of 10, 100 and 1000 ng/mL. Intra- and inter-day relative standard deviations were 4.01-7.30 and 4.15-7.51%, respectively. This method was successfully applied in the pharmacokinetic studies in rats.
Liu
Li
L
Key laboratory of Drug Metabolism and Pharmacokinetics, 24 Tong Jia Xiang Street, Mail Box 210, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, China.
Pang
Xiaoyan
X
Zhang
Dongmei
D
Xu
Xiao
X
Liu
Hanyan
H
Liang
Yan
Y
Xie
Lin
L
Liu
Xiaodong
X
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000737:chemistry; D004355:Drug Stability; D005215:Fasting; D024841:Fluoroquinolones / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D005615:Freezing; D006207:Half-Life; D007477:Ions; D007773:Lactates / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D016014:Linear Models; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature
D024841:Fluoroquinolones; D007477:Ions; D007773:Lactates; D002939:Ciprofloxacin