title: Angiostatin and integrin alphavbeta3 in the feline, bovine, canine, equine, porcine and murine retina and cornea.
abstract: OBJECTIVE
  Angiogenesis is tightly controlled in the ocular tissues of domestic animals but its mechanisms are not fully understood. This is largely because of insufficient data on the expression of molecules that impact angiogenesis. Because angiostatin and one of its receptors integrin alphavbeta3 inhibit and promote angiogenesis, respectively, we hypothesized that the normal retina and cornea of domestic animals would express angiostatin but not integrin alphavbeta3.
  
  
  METHODS
  Normal eyes of the cat, cow, dog, horse, pig and rat were evaluated for angiostatin and integrin alphavbeta3 by light and electron immunocytochemistry and estern blots.
  
  
  RESULTS
  Angiostatin was detected in the corneal epithelium of the cat, dog, horse, pig and rat, but was not found in cow corneal epithelium. Angiostatin was localized in the nerve fiber layer, ganglion cell layer, inner and outer plexiform layers, and the photoreceptor layer of the cat, cow, dog and rat. Horse and pig retinas showed additional staining in the matrix of the inner nuclear layer. Immunogold electron microscopy further confirmed angiostatin in cat retina. Western blots showed angiostatin in corneal and retinal homogenates. Integrin alphavbeta3 was absent in cornea and retina of all the species studied.
  
  
  CONCLUSIONS
  These data show that angiostatin, an inhibitor of angiogenesis, is present while integrin alphavbeta3, which promotes angiogenesis, is absent in normal cornea and retina of the domestic animals in this study with the exception being angiostatin absence in cow corneal epithelium. Therefore, angiostatin may contribute to the anti-angiogenic environment in the normal domestic animal eye while its absence in the cow may contribute to greater propensity for corneal vascularization. Because integrin alphavbeta3 is one of the receptors for angiostatin, its absence may prevent angiostatin from killing normal retinal and corneal cells.
authors.affiliation: Immunology Research Group, Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada.
authors.forename: Jacqueline W
authors.initials: JW
authors.lastname: Pearce
authors.forename: Kyathanahalli S
authors.initials: KS
authors.lastname: Janardhan
authors.forename: Sarah
authors.initials: S
authors.lastname: Caldwell
authors.forename: Baljit
authors.initials: B
authors.lastname: Singh
mesh_terms: D043168:Angiostatins / Q000032:analysis*; D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002415:Cats; D002417:Cattle; D003315:Cornea / Q000378:metabolism* / Q000648:ultrastructure; D004285:Dogs; D006736:Horses; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary; D039302:Integrin alphaVbeta3 / Q000032:analysis*; D051379:Mice; D012160:Retina / Q000378:metabolism* / Q000648:ultrastructure; D013045:Species Specificity; D013552:Swine
chemical_list: D039302:Integrin alphaVbeta3; D043168:Angiostatins