title: Determination of photoirradiated tetracyclines in water by high-performance liquid chromatography with chemiluminescence detection based reaction of rhodamine B with cerium (IV).
abstract: A simple, selective and sensitive method has been developed for the simultaneous determination of tetracycline, oxytetracycline, chlorotetracycline, demeclocycline, doxycycline and meclocycline based on reversed-phase high-performance liquid chromatography with chemiluminescence detection. The procedure was based on the chemiluminescent enhancement by photoirradiated tetracyclines of the cerium (IV)-rhodamine B system in sulphuric acid medium. The six tetracyclines were separated on an Aquasil-C18 column with a gradient elution using a mixture of acetonitrile and 0.1 mol L(-1) phosphate buffer as mobile phase, photoderivatized using a photoreactor consisting of a tube reactor coil of PFA and a 8W Xenon lamp. Under the optimized conditions, the method was validated with respect to linearity, precision, limits of detection and quantification and accuracy. The relative standard deviation (RSD) on intra-day precision was below 10% and detection limits ranged between 0.12 and 0.34 microg L(-1). The proposed method has been successfully applied to the determination of tetracyclines in surface water samples. A possible mechanism of the chemiluminescence in the system is discussed.
authors.affiliation: Department of Analytical Chemistry, Faculty of Experimental Sciences, University of Almeria, 04061 Almeria, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Santiago Valverde
authors.forename: I
authors.initials: I
authors.lastname: Sánchez Pérez
authors.forename: F
authors.initials: F
authors.lastname: Franceschelli
authors.forename: M
authors.initials: M
authors.lastname: Martínez Galera
authors.forename: M D
authors.initials: MD
authors.lastname: Gil García
mesh_terms: D002563:Cerium / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D007202:Indicators and Reagents; D007700:Kinetics; D008163:Luminescent Measurements / Q000379:methods*; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D010777:Photochemistry; D012235:Rhodamines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013754:Tetracyclines / Q000737:chemistry / Q000302:isolation & purification*; D014867:Water / Q000737:chemistry
chemical_list: D007202:Indicators and Reagents; D009942:Organometallic Compounds; D012235:Rhodamines; D013754:Tetracyclines; C506935:cerium(IV)-rhodamine 6G; D014867:Water; D002563:Cerium; C029773:rhodamine B