title: Real-time detection of DNA interactions with long-period fiber-grating-based biosensor.
abstract: Using an optical biosensor based on a dual-peak long-period fiber grating, we have demonstrated the detection of interactions between biomolecules in real time. Silanization of the grating surface was successfully realized for the covalent immobilization of probe DNA, which was subsequently hybridized with the complementary target DNA sequence. It is interesting to note that the DNA biosensor was reusable after being stripped off the hybridized target DNA from the grating surface, demonstrating a function of multiple usability.
authors.affiliation: School of Engineering and Applied Science, Aston University, Birmingham, B4 7ET, UK. chenx2@aston.ac.uk
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Kaiming
authors.initials: K
authors.lastname: Zhou
authors.forename: Edward
authors.initials: E
authors.lastname: Davies
authors.forename: Kate
authors.initials: K
authors.lastname: Sugden
authors.forename: Ian
authors.initials: I
authors.lastname: Bennion
authors.forename: Marcus
authors.initials: M
authors.lastname: Hughes
authors.forename: Anna
authors.initials: A
authors.lastname: Hine
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis*; D004867:Equipment Design; D004871:Erbium / Q000737:chemistry; D007368:Interferometry / Q000379:methods; D007834:Lasers; D008027:Light; D008962:Models, Theoretical; D009991:Oscillometry; D012666:Semiconductors; D013997:Time Factors; D015018:Ytterbium / Q000737:chemistry
chemical_list: D004871:Erbium; D004247:DNA; D015018:Ytterbium