[Surface-enhanced Raman spectra of oxidation damnification of fetal bovine serum by ozone].
Fetal bovine serum was treated by ozone for 1 hour and 3 hours before getting its surface-enhanced Raman spectra from 200 to 1 800 cm(-1). Treated with ozone for 1 hour, it shows a significant decrease in band intensity. Treated with ozone for 3 hours, the band intensity has a further decrease but not so obviously, which means that oxidation of ozone is short lived. Treated with ozone, the orderly conformations of main chains in protein such as alpha-helix, beta-sheet and beta-corner are damaged seriously. Aromatic side chains and C-S of Cys and Met also are damnified greatly. All this means that strong oxidation of ozone results in denaturation, conformational changes and even degradation in protein.
Zou
Zu-Quan
ZQ
Department of Biophysics, School of Physics, Nankai University, Tianjin 300071, China.
Liu
Yan-Nan
YN
Wu
Ying
Y
Li
Qi-Nan
QN
Chen
Mei-Zong
MZ
Sun
Hai-Ying
HY
Lee
Imshik
I
D000818:Animals; D001798:Blood Proteins / Q000737:chemistry; D002417:Cattle; D005312:Fetal Blood / Q000737:chemistry* / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D010126:Ozone / Q000737:chemistry* / Q000494:pharmacology; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D017433:Protein Structure, Secondary / Q000187:drug effects; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013997:Time Factors
D001798:Blood Proteins; D010126:Ozone