title: Association of a CD24 gene polymorphism with susceptibility to systemic lupus erythematosus.
abstract: OBJECTIVE
  To determine the potential role of the CD24 A57V gene polymorphism in systemic lupus erythematosus (SLE).
  
  
  METHODS
  We studied 3 cohorts of Caucasian patients and controls. The Spanish cohort included 696 SLE patients and 539 controls, the German cohort included 257 SLE patients and 317 controls, and the Swedish cohort included 310 SLE patients and 247 controls. The CD24 A57V polymorphism was genotyped by polymerase chain reaction, using a predeveloped TaqMan allele discrimination assay. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated.
  
  
  RESULTS
  In the Spanish cohort there was a statistically significant difference in the distribution of the CD24 V allele between SLE patients and controls (OR 3.6 [95% CI 2.13-6.16], P < 0.0001). In addition, frequency of the CD24 V/V genotype was increased in SLE patients compared with controls (OR 3.7 [95% CI 2.16-6.34], P < 0.00001). We sought to replicate this association with SLE in a German population and a Swedish population. A similar trend was found in the German group. The CD24 V/V genotype and the CD24 V allele were more frequent in SLE patients than in controls, although this difference was not statistically significant. No differences were observed in the Swedish group. A meta-analysis of the Spanish and German cohorts demonstrated that the CD24 V allele has a risk effect in SLE patients (pooled OR 1.25 [95% CI 1.08-1.46], P = 0.003). In addition, homozygosity for the CD24 V risk allele significantly increased the effect (pooled OR 2.19 [95% CI 1.50-3.22], P = 0.00007).
  
  
  CONCLUSIONS
  These findings suggest that the CD24 A57V polymorphism plays a role in susceptibility to SLE in a Spanish population.
authors.affiliation: Instituto de Parasitología y Biomedicina López-Neyra, CSIC, Granada, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Sánchez
authors.forename: Anna-Karin
authors.initials: AK
authors.lastname: Abelson
authors.forename: Jose M
authors.initials: JM
authors.lastname: Sabio
authors.forename: Miguel A
authors.initials: MA
authors.lastname: González-Gay
authors.forename: Norberto
authors.initials: N
authors.lastname: Ortego-Centeno
authors.forename: Juan
authors.initials: J
authors.lastname: Jiménez-Alonso
authors.forename: Enrique
authors.initials: E
authors.lastname: de Ramón
authors.forename: Julio
authors.initials: J
authors.lastname: Sánchez-Román
authors.forename: Miguel A
authors.initials: MA
authors.lastname: López-Nevot
authors.forename: Iva
authors.initials: I
authors.lastname: Gunnarsson
authors.forename: Elisabet
authors.initials: E
authors.lastname: Svenungsson
authors.forename: Gunnar
authors.initials: G
authors.lastname: Sturfelt
authors.forename: Lennart
authors.initials: L
authors.lastname: Truedsson
authors.forename: Andreas
authors.initials: A
authors.lastname: Jönsen
authors.forename: Maria Francisca
authors.initials: MF
authors.lastname: González-Escribano
authors.forename: Torsten
authors.initials: T
authors.lastname: Witte
authors.forename: Marta E
authors.initials: ME
authors.lastname: Alarcón-Riquelme
authors.forename: Javier
authors.initials: J
authors.lastname: Martín
mesh_terms: D051927:CD24 Antigen / Q000235:genetics*; D015331:Cohort Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic
chemical_list: D051927:CD24 Antigen