[Effect of ulinastain on the expression of hemeoxygenase-1 in oleic acid-induced acute lung injury in rats].
OBJECTIVE
To explore the effect of ulinastain on the expression of hemeoxy genase-1 (HO-1) in oil acid-induced acute lung injury in rats.


METHODS
The animal model of acute lung injury was established by oil acid. Thirty SD rats were randomly divided into 3 groups: the blank control group (A), the acute lung injury group (B) and the acute lung injury group (C) followed by injecting 100 mL/kg ulinastatin. Each group consisted of 10 rats. Group A were given 0.2 mL/kg natural solution through the trial vein; Group B and C were given 0.2 mL/kg oil-acid through trial vein, while group C were injected 100mL/kg ulinastatin by the peritoneal cavity after injecting oil acid. After 4 hours, the rates of respiration were counted and blood samples were cramped out through the heart puncture for blood gas analysis. The expressions of hemeoxygenase-1 and the pathologic construction changes were determined by HE staining in the lower right lung of rats in the 3 groups.


RESULTS
The respiration dysfunction caused by oil acid could be prominently improved by ulinastain. There was only a little expression of hemeoxygenase-1 in the lung of Group A, but the expression increased in Group B and significatively increased in Group C.


CONCLUSIONS
Ulinastatin may protect the rats from acute lung injury through increasing the expression of HO-1.
Jin
Li-Yan
LY
Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Xu
Jun-Mei
JM
He
Zhi-Biao
ZB
Ruan
Wen-Yan
WY
Chai
Xiang-Ping
XP
D055371:Acute Lung Injury / Q000139:chemically induced / Q000378:metabolism*; D000818:Animals; D006023:Glycoproteins / Q000494:pharmacology*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D019301:Oleic Acid / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley
D006023:Glycoproteins; D019301:Oleic Acid; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat; C028665:urinastatin