Characterization of bixin by LC-MS and LC-NMR.
An overview upon modern analytical techniques for the isolation, separation, and structural identification of the essential bioactive carotenoid bixin is given. Isolation from biological matrices is performed by matrix solid phase dispersion (MSPD). The extract is separated with shape-selective C(30 )columns. Structural assignment of the separated compounds is done by online LC-MS and capillary HPLC-NMR.
Rehbein
Jens
J
Institute of Organic Chemistry, University of Tuebingen, Tuebingen, Germany.
Dietrich
Benjamin
B
Grynbaum
Marc David
MD
Hentschel
Petra
P
Holtin
Karsten
K
Kuehnle
Maximilian
M
Schuler
Paul
P
Bayer
Marc
M
Albert
Klaus
K
D000096:Acetone / Q000032:analysis; D002338:Carotenoids / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods*; D004867:Equipment Design; D005609:Free Radicals; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D008956:Models, Chemical; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D020033:Protein Isoforms
D005609:Free Radicals; D010936:Plant Extracts; D020033:Protein Isoforms; D000096:Acetone; D002338:Carotenoids; C004588:bixin