title: Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism.
abstract: We previously demonstrated that a beta-1,3-, 1,6-oligoglucan (AaGlucan) from the fungus Alternaria alternata 102 shows strong elicitor activity in tobacco BY-2 cells. We have used cDNA microarray analysis to monitor global changes in gene expression in tobacco cells treated with this A. alternata fraction or with laminarin. In total, we identified 265 genes that were induced 1 h after treatment with an AaGlucan-enriched fraction or laminarin. Among them, we characterized in detail a novel tobacco R2R3 MYB-type transcription factor homolog (NtMYBGR1) and two DC1 domain-containing genes (NtDC1A and NtDC1B). Microarray data, together with overexpression and metabolic analyses, indicated that NtMYBGR1, but not the NtDC1 proteins, primarily targets the phenylpropanoid synthesis-related genes PAL and 4CL. These results suggest that NtMYBGR1 specifically regulates defense responses in BY-2 cells by enhancing phenylpropanoid metabolism in response to AaGlucan and laminarin elicitors.
authors.affiliation: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588 Japan. shinya@isc.meiji.ac.jp
authors.forename: Tomonori
authors.initials: T
authors.lastname: Shinya
authors.forename: Ivan
authors.initials: I
authors.lastname: Gális
authors.forename: Tomoko
authors.initials: T
authors.lastname: Narisawa
authors.forename: Mami
authors.initials: M
authors.lastname: Sasaki
authors.forename: Hiroo
authors.initials: H
authors.lastname: Fukuda
authors.forename: Hideaki
authors.initials: H
authors.lastname: Matsuoka
authors.forename: Mikako
authors.initials: M
authors.lastname: Saito
authors.forename: Ken
authors.initials: K
authors.lastname: Matsuoka
mesh_terms: D000528:Alternaria / Q000737:chemistry; D002460:Cell Line; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D005936:Glucans / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology; D020005:Propanols / Q000378:metabolism*; D014026:Nicotiana / Q000166:cytology*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D005936:Glucans; D010940:Plant Proteins; D011134:Polysaccharides; D020005:Propanols; D014157:Transcription Factors; C439395:1-phenylpropanol; C008247:laminaran