Crystallization and preliminary X-ray analysis of ginkbilobin-2 from Ginkgo biloba seeds: a novel antifungal protein with homology to the extracellular domain of plant cysteine-rich receptor-like kinases.
The antifungal protein ginkbilobin-2 (Gnk2) from Ginkgo biloba seeds does not show homology to other pathogenesis-related proteins, but does show homology to the extracellular domain of plant cysteine-rich receptor-like kinases. Native Gnk2 purified from ginkgo nuts and the selenomethionine derivative of recombinant Gnk2 (SeMet-rGnk2) were crystallized by the sitting-drop vapour-diffusion method using different precipitants. X-ray diffraction data were collected from Gnk2 at 2.38 A resolution and from SeMet-rGnk2 at 2.79 A resolution using a synchrotron-radiation source. The crystals of both proteins belonged to the primitive cubic space group P2(1)3, with unit-cell parameters a = b = c = 143.2 A.
Miyakawa
Takuya
T
Department of Applied Biochemical Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
Sawano
Yoriko
Y
Miyazono
Ken-ichi
K
Hatano
Ken-ichi
K
Tanokura
Masaru
M
D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D003545:Cysteine; D020441:Ginkgo biloba; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification; D010944:Plants / Q000201:enzymology; D011494:Protein Kinases / Q000737:chemistry / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry*; D014961:X-Ray Diffraction
D023181:Antimicrobial Cationic Peptides; D010940:Plant Proteins; C519081:ginkbilobin-2 protein, Ginkgo biloba; D011494:Protein Kinases; D003545:Cysteine