Systemic infection of stalks and ears of corn hybrids by Aspergillus parasiticus.
This study was conducted to explore systemic infection by the Aspergillus flavus group into corn ears via the stalk. An A. parasiticus mutant which produces norsolorinic (NOR) acid (a visible orange intermediate of the aflatoxin biosynthetic pathway) was used in field studies to monitor systemic infection of corn stalk and ear tissues. Corn hybrids resistant and susceptible to aflatoxin contamination were grown in the field and inoculated prior to tasseling by inserting A. parasiticus infested toothpicks into stalks between the 5th and 6th node below the lowest ear shoot. Beginning 2 weeks after inoculation, systemic infection by the NOR mutant was assessed weekly by collecting ear shank tissue and stalk tissue from the nodes between the infection sites and the developing ears. Ears were collected at the end of the growing season to determine the level of kernel infection by the NOR mutant. In two separate studies, the A. parasiticus NOR mutant was isolated from stalk tissues at all of node positions and ear shank tissue from several susceptible corn hybrid plants at the first harvest date 2 weeks after inoculation. The NOR mutant was also isolated from stalk and ear tissue of a resistant hybrid. The NOR mutant was only isolated from kernels of susceptible hybrids in 2003 and 2004. Infection rates of kernels in infected ears were very low (<1%). In 2005, the fungus was found in only one kernel from an ear of the resistant hybrid. The NOR mutant was not isolated from stalks, ears, or kernels from control (uninoculated) plants grown in the plots with inoculated plants. Although infection levels of corn kernels were low, systemic movement of the A. parasiticus up the stalk appears to be another possible route to infection of developing corn ears.
Windham
Gary L
GL
USDA-ARS, Corn Host Plant Resistance Research Unit, PO Box 5367, Mississippi State, MS 39762, USA. glwindham@msa-msstate.ars.usda.gov
Williams
William P
WP
D001230:Aspergillus / Q000235:genetics / Q000254:growth & development*; D006824:Hybridization, Genetic; D007113:Immunity, Innate / Q000235:genetics; D009154:Mutation; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018514:Plant Structures / Q000235:genetics / Q000382:microbiology*; D012621:Seasons; D003313:Zea mays / Q000235:genetics / Q000382:microbiology*