title: Nontypeable Haemophilus influenzae and Streptococcus pneumoniae bind respiratory syncytial virus glycoprotein.
abstract: Respiratory syncytial virus (RSV) infection is associated with secondary bacterial infections caused by nontypeable Haemophilus influenzae (NTHi) and Streptococcus pneumoniae. The pathogenesis of these complications is not completely understood; however, viral infection of respiratory epithelial cells promotes colonization by these bacteria. In the present study, RSV virions associated with NTHi and pneumococci in an inoculum-dependent manner in a fluid-phase binding assay. Adherence of NTHi and S. pneumoniae to epithelial cells transiently expressing RSV G glycoprotein was 2- and 2.2-fold higher, respectively, than adhesion to cells transfected with the vector alone (P <0.01). Furthermore, 4.6- and 6.2-fold larger numbers of NTHi and pneumococci bound to cells expressing a membrane-bound full-length RSV G protein than to cells expressing a truncated non-membrane-bound protein (P <or=0.005). Pre-incubating cells expressing membrane-bound G protein with blocking anti-RSV G antibodies reduced bacterial adherence by 78-84 % (P <or=0.005). These studies demonstrate that RSV G protein is a receptor for both NTHi and S. pneumoniae. Strategies to prevent this interaction may reduce the incidence of secondary bacterial complications of RSV infection.
authors.affiliation: Department of Molecular Sciences, University of Tennessee Health Sciences Center, Memphis, TN 38163, USA.|Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
authors.forename: Vasanthi
authors.initials: V
authors.lastname: Avadhanula
authors.affiliation: Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Molecular Sciences, University of Tennessee Health Sciences Center, Memphis, TN 38163, USA.|Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
authors.forename: Allen
authors.initials: A
authors.lastname: Portner
authors.affiliation: Department of Pediatrics, University of Tennessee Health Sciences Center, Memphis, TN 38163, USA.|Department of Molecular Sciences, University of Tennessee Health Sciences Center, Memphis, TN 38163, USA.|Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Adderson
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004847:Epithelial Cells / Q000382:microbiology / Q000821:virology; D006192:Haemophilus Infections / Q000382:microbiology; D006193:Haemophilus influenzae / Q000145:classification / Q000502:physiology*; D011008:Pneumococcal Infections / Q000382:microbiology; D011485:Protein Binding; D018357:Respiratory Syncytial Virus Infections / Q000150:complications; D013296:Streptococcus pneumoniae / Q000502:physiology*; D014760:Viral Fusion Proteins / Q000378:metabolism*
chemical_list: C502275:G glycoprotein, Respiratory syncytial virus; D014760:Viral Fusion Proteins