[Expression and biological activities of arresten in CHO cells].
OBJECTIVE
To explore the eukaryotic expression of arresten in CHO cells and to investigate its basic biological activities.


METHODS
CHO cells were divided into three groups: transfected pSecTag-arresten group, transfected pSecTag group and control group without transfection. PSecTag-arresten was transfected into CHO cells by Lipofectamine 2000 method. The arresten mRNA in CHO cells was assayed by RT-PCR. The protein expression of arresten gene was examined by Western-Blot. The cells expressing arresten were screened out by Zeocin. The effect of arresten on huvec cell migration and anchoring to three-dimensional vascular structures was measured.


RESULTS
The result of RT-PCR and Western-blot showed that arresten gene has been successfully transfected into CHO cells and expressed in those cells. Arrssten inhibited huvec cell migration and anchoring to three-dimensional vascular structures.


CONCLUSIONS
CHO cells expressing arresten have been obtained successfully. Arresten can inhibit huvec cell migration and anchoring to three-dimensional vascular structures, indicating that it might be one of its anti-angiogenetic approaches.
Long
Miao-yun
MY
Department of Hepatobiliary Surgery, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. lmy1023@sohu.com
Zheng
Qi-chang
QC
Song
Zi-fang
ZF
Hu
Qing-gang
QG
Zhang
Yong
Y
D020533:Angiogenesis Inhibitors / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western; D016466:CHO Cells; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D020533:Angiogenesis Inhibitors; D024141:Collagen Type IV; D012333:RNA, Messenger; D011994:Recombinant Proteins