[Culture of chondrocytes using nano-hydroxyapatite/collagen in vitro].
OBJECTIVE
To evaluate the value of the nano-hydroxyapatite/ collagen (nHAC) as a scaffold for cartilage tissue engineering.


METHODS
The normal cartilage from patients after total hip arthroplasty of osteoarthritis was selected, and then chondrocytes were digested, separated, and amplificate in vitro. The chondrocytes were seeded onto the nHAC scaffold and were cultured in a 3-dimensional environment for 5 and 10 days. The effects of the composite scaffold on the cell adhesion, proliferation, morphological changes, and synthesis of the extracellular matrix were observed by scanning electronic microscopy and immunohistochemistry.


RESULTS
The chondrocytes could adhere to the surface of the scaffolds, proliferate, and migrate into the scaffolds. They maintained round and could secrete extracellular matrix on the porous scaffold.


CONCLUSIONS
The nHAC can be used as the cartilage cell carrier.
Xiao
Tao
T
Research Institute of Traumatic Orthopaedics, Second Xiangya Hospital, Central South University, Changsha 410011, China. xiaotaoxyl@163.com
Peng
Cheng
C
Luo
Yuan-Ming
YM
Lin
Mian-Hui
MH
Liu
Xia-Jun
XJ
D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D003094:Collagen; D017886:Durapatite; D006801:Humans; D019736:Prostheses and Implants; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
C509317:nano-hydroxyapatite-collagen; D003094:Collagen; D017886:Durapatite