title: Crystallization and preliminary X-ray diffraction analysis of the small subunit of the heterodimeric restriction endonuclease R.BspD6I.
abstract: The heterodimeric restriction endonuclease R.BspD6I is composed of a small subunit with a cleavage site and a large subunit, containing a recognition domain and a cleavage domain, that may function separately as a monomeric nicking endonuclease. Here, the crystallization of the small subunit and diffraction data collection to 1.5 A resolution are reported.
authors.affiliation: Max-Planck Unit for Structural Molecular Biology, Hamburg 22607, Germany.
authors.forename: Galina S
authors.initials: GS
authors.lastname: Kachalova
authors.forename: Alfiya K
authors.initials: AK
authors.lastname: Yunusova
authors.forename: Rimma I
authors.initials: RI
authors.lastname: Artyukh
authors.forename: Eugeny A
authors.initials: EA
authors.lastname: Rogulin
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Perevyazova
authors.forename: Ludmila A
authors.initials: LA
authors.lastname: Zheleznaya
authors.forename: Nickolay I
authors.initials: NI
authors.lastname: Matvienko
authors.forename: Hans D
authors.initials: HD
authors.lastname: Bartunik
mesh_terms: D003460:Crystallization; D004262:DNA Restriction Enzymes / Q000737:chemistry / Q000302:isolation & purification; D003850:Deoxyribonuclease I / Q000737:chemistry* / Q000302:isolation & purification; D019281:Dimerization; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000302:isolation & purification; D021122:Protein Subunits / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D029968:Escherichia coli Proteins; D021122:Protein Subunits; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I; C514554:BspD6I protein, E coli