title: Sequential multiplex PCR for identifying pneumococcal capsular serotypes from South-Saharan African clinical isolates.
abstract: A serial multiplex PCR approach was reformulated for pneumococcal serotyping to test 153 clinical isolates from children in Mozambique. This approach identified serotypes in 139 (90.8%) of 153 isolates; 126 (82.4%) were identified within two reactions. This approach in developing countries would require minimal training and could provide useful serotype information without requiring transport of specimens.
authors.affiliation: Centro de Investigação em Saúde da Manhiça (CISM), Ministerio de Saúde, CP1929 Maputo, Mozambique.
authors.forename: Luis
authors.initials: L
authors.lastname: Morais
authors.affiliation: Respiratory Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Maria da Glória
authors.initials: MDG
authors.lastname: Carvalho
authors.affiliation: Centre de Salut Internacional (CSI), Hospital Clinic/IDIBAPS, Universitat de Barcelona, Barcelona, Spain.|Centro de Investigação em Saúde da Manhiça (CISM), Ministerio de Saúde, CP1929 Maputo, Mozambique.
authors.forename: Anna
authors.initials: A
authors.lastname: Roca
authors.affiliation: Respiratory Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Brendan
authors.initials: B
authors.lastname: Flannery
authors.affiliation: Instituto Nacional de Saúde Ministério de Saúde, Maputo, Mozambique.|Centro de Investigação em Saúde da Manhiça (CISM), Ministerio de Saúde, CP1929 Maputo, Mozambique.
authors.forename: Inacio
authors.initials: I
authors.lastname: Mandomando
authors.affiliation: Respiratory Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Soriano-Gabarró
authors.affiliation: Instituto Nacional de Saúde Ministério de Saúde, Maputo, Mozambique.|Centro de Investigação em Saúde da Manhiça (CISM), Ministerio de Saúde, CP1929 Maputo, Mozambique.
authors.forename: Betuel
authors.initials: B
authors.lastname: Sigauque
authors.affiliation: Centre de Salut Internacional (CSI), Hospital Clinic/IDIBAPS, Universitat de Barcelona, Barcelona, Spain.|Centro de Investigação em Saúde da Manhiça (CISM), Ministerio de Saúde, CP1929 Maputo, Mozambique.
authors.forename: Pedro
authors.initials: P
authors.lastname: Alonso
authors.affiliation: Respiratory Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Bernard
authors.initials: B
authors.lastname: Beall
mesh_terms: D000293:Adolescent; D000942:Antigens, Bacterial / Q000235:genetics*; D016667:Bacterial Capsules / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D005838:Genotype; D006801:Humans; D007223:Infant; D009073:Mozambique / Q000453:epidemiology; D011008:Pneumococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping / Q000379:methods; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000942:Antigens, Bacterial