title: The toxicosis and detoxifcation of anionic/cationic surfactants targeted to bovine serum albumin.
abstract: By using the techniques of resonance light scattering (RLS) and absorption spectra, we studied the toxicosis and detoxifcation mechanism of anionic surfactant SDBS and cationic surfactant CTMAB targeted to bovine serum albumin (BSA). Small quantity of CTMAB combines with SDBS-BSA complex to form SDBS-BSA-CTMAB complex and the IRLS of system enhanced greatly. With the cumulation of quantity, CTMAB captures SDBS from SDBS-BSA complex by electrostatic attraction and CTMAB-SDBS complex forms, meanwhile BSA automatically frees, which is corresponding to the toxicosis and detoxifcation process. Absorption experiment validates that SDBS induces the denaturalization of BSA and CTMAB facilitates the refolding of this protein.
authors.affiliation: School of Environmental Science and Engineering, Shandong University, Jinan 250100, PR China. rutaoliu@sdu.edu.cn
authors.forename: Rutao
authors.initials: R
authors.lastname: Liu
authors.forename: Wansong
authors.initials: W
authors.lastname: Zong
authors.forename: Kaikai
authors.initials: K
authors.lastname: Jin
authors.forename: Xuetang
authors.initials: X
authors.lastname: Lu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhu
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhang
authors.forename: Canzhu
authors.initials: C
authors.lastname: Gao
mesh_terms: D000042:Absorption; D019342:Acetic Acid / Q000737:chemistry; D000818:Animals; D000838:Anions; D002021:Buffers; D002412:Cations; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008027:Light; D011489:Protein Denaturation / Q000187:drug effects; D017433:Protein Structure, Secondary; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000032:analysis*; D019346:Sodium Acetate / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D055672:Static Electricity; D013501:Surface-Active Agents / Q000737:chemistry* / Q000633:toxicity*; D014867:Water / Q000737:chemistry
chemical_list: D000838:Anions; D002021:Buffers; D002412:Cations; D013501:Surface-Active Agents; D014867:Water; D012710:Serum Albumin, Bovine; D019346:Sodium Acetate; D019342:Acetic Acid