title: Sequential multiplex PCR for determining capsular serotypes of pneumococci recovered from Brazilian children.
abstract: Capsular serotype surveillance of clinical isolates of Streptococcus pneumoniae is essential for evaluation of the potential impact of introducing multivalent capsular serotype-based vaccines in Latin America. Here, a previously described sequential multiplex PCR method was revised for optimal targeting of prevalent serotypes in Latin America. The revised protocol successfully serotyped 139/147 pneumococci (94.6%) from Brazilian children, demonstrating a labour-efficient, accurate method requiring only conventional PCR capability.
authors.affiliation: Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil.|Fundação Faculdade Federal de Ciências Médicas de Porto Alegre, and Hospital Mãe de Deus, Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Cícero A
authors.initials: CA
authors.lastname: Dias
authors.affiliation: Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil.
authors.forename: Lúcia Martins
authors.initials: LM
authors.lastname: Teixeira
authors.affiliation: Respiratory Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Maria da Glória
authors.initials: MDG
authors.lastname: Carvalho
authors.affiliation: Respiratory Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
authors.forename: Bernard
authors.initials: B
authors.lastname: Beall
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D016667:Bacterial Capsules / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D001938:Brazil / Q000453:epidemiology; D002675:Child, Preschool; D005838:Genotype; D006801:Humans; D007223:Infant; D011008:Pneumococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping / Q000379:methods; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000942:Antigens, Bacterial