title: Expression Profile of Wnt Molecules in Leukemic Cells from Iranian Patients with Acute Myeloblastic Leukemia
abstract: Wnt molecules play a key role in growth, proliferation and development of some embryonic and adult organs as well as hematopoietic stem cells. Wnt signaling pathways are aberrantly activated in many tumor types, including solid tumors and hematologic malignancies.
  
  
  
  To investigate the expression profile of a large number of Wnt genes in leukemic cells from Iranian patients with acute myeloblastic leukemia.
  
  
  
  RT-PCR method was used to determine the Wnt genes expression in bone marrow (BM) and/or peripheral blood (PB) samples from 16 patients with AML and PB samples of 36 normal subjects.
  
  
  
  Among 14 Wnt molecules included in this study, Wnt-7A and Wnt-10A were significantly down-regulated (p = 0.002 and p < 0.0001, respectively) and Wnt-3 was significantly over-expressed (p < 0.02) in AML patients compared to normal subjects. No significant association was found between Wnt expression and FAB classification of the patients.
  
  
  
  Our results demonstrated for the first time aberrant expression of Wnt-7A, Wnt-10A and Wnt-3 genes in Iranian AML patients. This may be of relevance to the tumorigenesis process in this malignancy.
authors.affiliation: Department of Immunology, School of Public Health, Medical Sciences, University of Tehran, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Memarian
authors.forename: Mahmood
authors.initials: M
authors.lastname: Jeddi Tehrani
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Vossough
authors.forename: Ramazan Ali
authors.initials: RA
authors.lastname: Sharifian
authors.forename: Hodjatallah
authors.initials: H
authors.lastname: Rabbani
authors.forename: Fazel
authors.initials: F
authors.lastname: Shokri
mesh_terms: D000293:Adolescent; D000328:Adult; D001854:Bone Marrow Cells / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007223:Infant; D007492:Iran; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism*; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051153:Wnt Proteins / Q000097:blood / Q000235:genetics* / Q000378:metabolism
chemical_list: D051153:Wnt Proteins