title: Electroacupuncture at acupoint ST-36 promotes contractility of distal colon via a cholinergic pathway in conscious rats.
abstract: OBJECTIVE
  To investigate the effects and possible mechanism of electroacupuncture (EA) at acupoint ST-36 (Zusanli) on the contractility of the distal colon in conscious rats.
  
  
  METHODS
  Rats were randomized into four groups, including the ST-36 group, the sham group, the saline-plus-ST-36 and the atropine-plus-ST-36 group. Needles were inserted into the acupoints for EA. The distal colonic contractility was measured with a manometric catheter inserted into the distal colon. The colonic contractility was recorded for 1 h before EA, 20 min during EA, and 3 h after EA. Saline or atropine was administrated in the saline-plus-ST-36 or atropine-plus-ST-36 group.
  
  
  RESULTS
  (1) EA at ST-36 significantly increased the contractility of the distal colon. The mean AUC during and after EA were significantly higher than that before EA in the ST-36 group, a 27% increase during EA and 26% after EA. (2) Atropine abolished the accelerating effect of EA at ST-36. The mean AUC remained unchanged during or after EA compared with that before EA in the atropine-plus-ST-36 group.
  
  
  CONCLUSIONS
  EA at ST-36 has a stimulatory effect on the contractility of the distal colon in conscious rats, and the stimulatory effect may be mediated via the cholinergic pathway.
authors.affiliation: Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Luo
authors.forename: Shi
authors.initials: S
authors.lastname: Liu
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Xie
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Hou
mesh_terms: D000818:Animals; D002799:Cholinergic Fibers / Q000502:physiology*; D003106:Colon / Q000502:physiology*; D015671:Electroacupuncture; D005769:Gastrointestinal Motility / Q000502:physiology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley