Lipid rafts, fluid/fluid phase separation, and their relevance to plasma membrane structure and function.
Novel biophysical approaches combined with modeling and new biochemical data have helped to recharge the lipid raft field and have contributed to the generation of a refined model of plasma membrane organization. In this review, we summarize new information in the context of previous literature to provide new insights into the spatial organization and dynamics of lipids and proteins in the plasma membrane of live cells. Recent findings of large-scale separation of liquid-ordered and liquid-disordered phases in plasma membrane vesicles demonstrate this capacity within the complex milieu of plasma membrane proteins and lipids. Roles for membrane heterogeneity and reorganization in immune cell activation are discussed in light of this new information.
Sengupta
Prabuddha
P
Department of Chemistry and Chemical Biology, Baker Laboratory, Cornell University, Ithaca, NY 14853-1301, United States.
Baird
Barbara
B
Holowka
David
D
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry* / Q000502:physiology* / Q000648:ultrastructure; D002477:Cells / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D008560:Membrane Fluidity / Q000502:physiology*; D021962:Membrane Microdomains / Q000737:chemistry / Q000502:physiology*; D044367:Phase Transition; D015398:Signal Transduction / Q000502:physiology; D053835:Unilamellar Liposomes / Q000737:chemistry
D053835:Unilamellar Liposomes