Online concentration of aristolochic acid I and II in Chinese medicine preparations by micellar electrokinetic chromatography.
In this study, an online concentration method in micellar electrokinetic chromatography (MEKC) applying field-enhanced sample injection (FESI) mode was developed for the detection of aristolochic acids (AAs) in Chinese medicine preparations. AA-I and AA-II were baseline separated with high separation efficiency, and 100-fold enhancement of the detection sensitivity was achieved compared with those obtained from normal capillary zone electrophoresis (CZE) or simple MEKC method. The proposed method was successfully applied for the determination of AAs in Chinese medicine preparations.
Shi
Shenhua
S
Beijing National Laboratory for Molecular Science, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Li
Wei
W
Liao
Yiping
Y
Cai
Zongwei
Z
Liu
Huwei
H
D034341:Aristolochic Acids / Q000737:chemistry / Q000302:isolation & purification*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation* / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D031521:Fractionation, Field Flow / Q000379:methods; D000432:Methanol; D015394:Molecular Structure; D009862:Online Systems; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D034341:Aristolochic Acids; D004365:Drugs, Chinese Herbal; D000432:Methanol