title: Vgamma9Vdelta2 T cell-mediated recognition of human solid tumors. Potential for immunotherapy of hepatocellular and colorectal carcinomas.
abstract: BACKGROUND
  Vgamma9Vdelta2 T lymphocytes are reported to participate in the anti-tumor immune surveillance in human. They are known to recognize phosphoantigens and molecules expressed on cells undergoing neoplasic transformation. In this study, we investigated phenotype and anti-tumor cytotoxicity of ex vivo expanded Vgamma9Vdelta2 T cells in view of adoptive immunotherapy.
  
  
  METHODS
  Experiments were performed with peripheral blood samples from eleven patients [six colorectal carcinoma (CRC), four hepatocellular carcinoma (HCC), one sarcoma] and sixteen healthy donors.
  
  
  CONCLUSIONS
  Ex vivo expansion of Vgamma9Vdelta2 T cells could be achieved by a single dose of phosphoantigen, either bromohydrin pyrophosphate or zoledronate, and supported by exogenous IL-2. After 2 weeks, expanded Vgamma9Vdelta2 T lymphocytes acquired the effector memory phenotype CD45RA(-)CD45RO(high)CD27(-). They expressed NKG2D and CD161 and the proinflammatory CXCR3 and CCR5 chemokine receptors. Vgamma9Vdelta2 T cells displayed a strong lytic activity toward a broad panel of tumor cell lines or primary cultures. Interestingly, HCC and CRC primary cells could be lysed by autologous Vgamma9Vdelta2 T cells whereas autologous normal cells were not sensitive to the lysis. mAbs blocking assays demonstrated that TCR was the most important receptor involved in the lysis of tumor cells. However, NKG2D receptor could deliver a costimulatory signal enhancing the lysis of HCC and CRC tumors expressing MICA/B. Treatment of tumor cells by the mevalonate pathway inhibitor, zoledronate, enhanced the killing of both HCC and CRC. Expansion index of Vgamma9Vdelta2 T cells was in similar levels in healthy donors or in cancer patients and total expansion was suitable for adoptive immunotherapy.
  
  
  CONCLUSIONS
  These results provide a rationale for the clinical evaluation of Vgamma9Vdelta2 T lymphocytes in HCC and CRC.
authors.affiliation: UPRES EA 3891, Faculté de Médecine de Rennes, 2 Avenue du Pr Léon Bernard, CS34317, 35043, Rennes cedex, France.
authors.forename: Francoise
authors.initials: F
authors.lastname: Bouet-Toussaint
authors.forename: Florian
authors.initials: F
authors.lastname: Cabillic
authors.forename: Olivier
authors.initials: O
authors.lastname: Toutirais
authors.forename: Matthieu
authors.initials: M
authors.lastname: Le Gallo
authors.forename: Cécile
authors.initials: C
authors.lastname: Thomas de la Pintière
authors.forename: Pascale
authors.initials: P
authors.lastname: Daniel
authors.forename: Noëlle
authors.initials: N
authors.lastname: Genetet
authors.forename: Bernard
authors.initials: B
authors.lastname: Meunier
authors.forename: Eric
authors.initials: E
authors.lastname: Dupont-Bierre
authors.forename: Karim
authors.initials: K
authors.lastname: Boudjema
authors.forename: Véronique
authors.initials: V
authors.lastname: Catros
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000276:immunology* / Q000628:therapy; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000628:therapy; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D006801:Humans; D007156:Immunologic Memory; D016219:Immunotherapy, Adoptive / Q000379:methods*; D008113:Liver Neoplasms / Q000276:immunology* / Q000628:therapy; D008213:Lymphocyte Activation / Q000276:immunology; D055655:NK Cell Lectin-Like Receptor Subfamily K; D010641:Phenotype; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D011971:Receptors, Immunologic / Q000276:immunology; D055607:Receptors, Natural Killer Cell; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: C527085:KLRK1 protein, human; D055655:NK Cell Lectin-Like Receptor Subfamily K; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011971:Receptors, Immunologic; D055607:Receptors, Natural Killer Cell