[3 polymorphisms of gene GP IIb and the function of GP IIb T13959 G in the platelet transfusion refractoriness].
OBJECTIVE
To investigate the relationship among 3 polymorphisms of GP IIb and the function of GP IIb T13959 G in the platelet transfusion refractoriness(PTR).


METHODS
The 26th exon, the 30th exon and the 21st intron of gene GP IIb in 110 individuals were amplified by polymerase chain reaction (PCR), and the PCR products were analyzed with single-strand conformation polymorphism(SSCP) and sequenced to investigate whether there was linkage among the polymorphisms of the gene. Human platelet antigen-3 (HPA-3) gene frequency was detected by Fok I enzyme in 147 patients with hematologic diseases, and was compared with that in 110 normal individuals. Forty-four patients who received apheresis platelet transfusion repeatedly were randomly divided into the HPA-3 homotype group and the control group. The antibodies of the platelet were detected after 3 times of platelet transfusion.


RESULTS
There were polymorphisms of gene GP IIb in the 26th, 30th exon and the 21st intron, and the mutations were: T changed into G in 13,959 th of the 26th exon; C changed into T in 16,997 th of the 30th exon; the 9 bps deletion occurred in 11,996-12,004 th of the 21st intron. The 3 polymorphisms had synchronization in the individuals. The results of Fok I enzyme indicated that the frequency of HPA-3a was 83.6% (92/110)and 81.9%(119/147), and that of HPA-3b was 16.4%(18/110) and 19.1%(28/147) in the normal individuals and the patients respectively. There was no significant difference between the patients and normal individuals (P>0.05). After the platelet transfusion, the antibodies of all the cases of the homotype platelet transfusion were negative, but the antibodies in 2 cases of the control group were positive, and there was antibody to HPA-3a in one of the antibodies positive cases.


CONCLUSIONS
(1)There is close linkage among the polymorphisms of gene GP IIb, which is T->G in 13 959 th of the 26th exon, C->T in 16,997 th of the 30th exon, and the 9 bps deletion in 11,996-12,004 th in the 21st intron. (2)The gene frequency of HPA-3a/3b is similar in the normal individuals and patients with hematologic diseases. (3) HPA-3 system may be one of the reasons for PTR in Chinese.
Liu
Jing
J
Department of Hematology, Department of Blood Transfusion, Third Xiangya Hospital, Central South University, Changsha 410013, China. jingliu0318@yahoo.com.cn
Chen
Fang-Ping
FP
Ma
Jin-Qi
JQ
Jian
Zai-Fu
ZF
Xing
Xiao-Wei
XW
Li
Xin
X
Jiang
Tie-Bin
TB
Zhou
Yang
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D016824:Antigens, Human Platelet / Q000276:immunology / Q000502:physiology*; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002648:Child; D005091:Exons; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007108:Immune Tolerance; D007438:Introns; D008297:Male; D008875:Middle Aged; D040201:Platelet Membrane Glycoprotein IIb / Q000235:genetics* / Q000276:immunology*; D017713:Platelet Transfusion; D018807:Polymorphism, Single-Stranded Conformational; D055815:Young Adult
C044561:3a alloantigen, human; C083448:3b alloantigen, human; D016824:Antigens, Human Platelet; D040201:Platelet Membrane Glycoprotein IIb