title: Monitoring neutrophil engraftment in allogeneic stem cell transplantation by flow cytometric analysis of neutrophil-specific antigens NA1 and NA2.
abstract: Neutrophil-specific antigen (NA) expression on neutrophils was analysed in 18 Japanese children before and after allogeneic stem cell transplantation (allo-SCT) with myeloablative regimen. Donor-recipient NA-incompatibility was present in one of eight NA1/NA2 heterozygous patients and eight of 10 NA1/NA1 or NA2/NA2 homozygous patients. After allo-SCTs from NA-incompatible donors, a neutrophil recipient-to-donor conversion was confirmed in all cases. Conversion to donor NA type was complete before the absolute neutrophil count reached 0.1 x 10(9)/l. These observations indicate that flow cytometric analysis of NA antigens is a simple and useful method for monitoring neutrophil engraftment in NA-incompatible allo-SCT.
authors.affiliation: Department of Paediatrics, School of Medicine, University of Yamanashi, Yamanashi, Japan. tinukai@yamanashi.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Inukai
authors.forename: Kanako
authors.initials: K
authors.lastname: Uno
authors.forename: Kikuyo
authors.initials: K
authors.lastname: Taniguchi
authors.forename: Kumiko
authors.initials: K
authors.lastname: Goi
authors.forename: Toru
authors.initials: T
authors.lastname: Tezuka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nemoto
authors.forename: Kazuya
authors.initials: K
authors.lastname: Takahashi
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sato
authors.forename: Koshi
authors.initials: K
authors.lastname: Akahane
authors.forename: Kinuko
authors.initials: K
authors.lastname: Hirose
authors.forename: Hiroko
authors.initials: H
authors.lastname: Honna
authors.forename: Keiko
authors.initials: K
authors.lastname: Kagami
authors.forename: Asako
authors.initials: A
authors.lastname: Hiraoka
authors.forename: Misae
authors.initials: M
authors.lastname: Tanihiro
authors.forename: Shinpei
authors.initials: S
authors.lastname: Nakazawa
authors.forename: Kanji
authors.initials: K
authors.lastname: Sugita
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D002648:Child; D005434:Flow Cytometry; D006085:Graft Survival; D006801:Humans; D007519:Isoantigens / Q000032:analysis*; D009504:Neutrophils / Q000378:metabolism / Q000637:transplantation*; D033581:Stem Cell Transplantation / Q000379:methods*; D013995:Time; D014184:Transplantation, Homologous
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D007519:Isoantigens; C067704:neutrophil-specific antigen NA1, human; C072753:neutrophil-specific antigen NA2