title: Growth of tobacco protoplasts stimulated by synthetic lipo-chitooligosaccharides.
abstract: fat Nodulation (Nod) factors are lipo-chitooligosaccharides (LCOs) secreted by rhizobia to trigger the early steps of nodule organogenesis in leguminous plants. A method to synthesize LCOs in vitro was developed. Synthetic LCOs alleviated the requirement for auxin and cytokinin to sustain growth of cultured tobacco protoplasts. LCOs containing C(18:1) trans-fatty acyl substituents were more effective than those containing cis-fatty acids in promoting cell division as well as in activating an auxin-responsive promoter and the expression of a gene implicated in auxin action. These data indicate that LCOs redirect plant growth also in nonlegumes by activating developmental pathways also targeted by phytohormones.
authors.forename: H
authors.initials: H
authors.lastname: Röhrig
authors.forename: J
authors.initials: J
authors.lastname: Schmidt
authors.forename: R
authors.initials: R
authors.lastname: Walden
authors.forename: I
authors.initials: I
authors.lastname: Czaja
authors.forename: E
authors.initials: E
authors.lastname: Miklascaroneviccarons
authors.forename: U
authors.initials: U
authors.lastname: Wieneke
authors.forename: J
authors.initials: J
authors.lastname: Schell
authors.forename: M
authors.initials: M
authors.lastname: John