Ultrasensitive assay of clindamycin in medicine and bio-fluids with chemiluminescence detection.
A simple chemiluminescence (CL) method using flow injection has been developed for the determination of clindamycin, based on the inhibitory effect of clindamycin on the CL generated from the luminol-K(3)Fe(CN)(6) system in alkaline medium. It was found that the decrement of CL intensity was linear with the logarithm of clindamycin concentration over the range 0.7-1000 ng/mL. The detection limit was 0.2 ng/mL with a relative standard deviation (RSD) of <5.0% (n = 7). At a flow rate of 3.0 mL/min, a complete analytical process could be performed within 0.5 min, including sampling and washing. The proposed procedure was applied successfully to the determination of clindamycin in pharmaceutical preparations and human urine without pretreatment.
Wei
Gaili
G
Department of Chemistry, Northwest University, Xi'an, People's Republic of China. weigaili123212@163.com
Dang
Gaochao
G
Li
Hua
H
D000900:Anti-Bacterial Agents / Q000652:urine*; D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D001826:Body Fluids / Q000737:chemistry*; D002981:Clindamycin / Q000652:urine*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000652:urine*; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D004364:Pharmaceutical Preparations; D002981:Clindamycin; D008165:Luminol