title: Poor diagnostic accuracy of a single fasting plasma citrulline concentration to assess intestinal energy absorption capacity.
abstract: OBJECTIVE
  Our aim was to explore the diagnostic value of fasting citrulline concentrations to detect decreased intestinal energy absorption in patients with recently diagnosed celiac disease (CeD), refractory celiac disease (RCeD), and short bowel syndrome (SBS). Decreased intestinal energy absorption is regarded a marker of intestinal failure.
  
  
  METHODS
  Fasting plasma citrulline concentrations were determined by high performance liquid chromatography (HPLC) in a prospective study of 30 consecutive adult patients (15 CeD, 9 RCeD, and 16 SBS) and 21 healthy subjects. Intestinal energy absorption capacity using bomb calorimetry was determined in all patients and healthy subjects and was regarded as the gold standard for intestinal energy absorption function.
  
  
  RESULTS
  The mean fasting plasma citrulline concentration was lower in RCeD patients than in healthy subjects (28.5+/-9.9 vs 38.1+/-8.0 micromol/L, P<0.05) and CeD patients (28.5+/-9.9 vs 38.1+/-6.4 micromol/L, P<0.05), however, clearly within reference values. The mean intestinal energy absorption capacity was lower in SBS patients than in healthy subjects (64.3+/-18.2 vs 90.3+/-3.5%, P<0.001), CeD patients (64.3+/-18.2 vs 89.2+/-3.4%, P<0.001), and the RCeD group (64.3+/-18.2 vs 82.3+/-11.7%, P<0.01). No relation was observed between fasting plasma citrulline concentration and intestinal energy absorption capacity (Pearson r=0.09, P=0.56). The area under the ROC curve for fasting plasma citrulline to detect decreased intestinal energy absorption capacity (i.e., <85%) was 0.50.
  
  
  CONCLUSIONS
  Fasting plasma citrulline concentrations have poor test characteristics for detection of decreased intestinal energy absorption capacity in patients with enterocyte damage.
authors.affiliation: Department of Gastroenterology, Small Bowel Unit, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Job H C
authors.initials: JH
authors.lastname: Peters
authors.forename: Nicolette J
authors.initials: NJ
authors.lastname: Wierdsma
authors.forename: Tom
authors.initials: T
authors.lastname: Teerlink
authors.forename: Paul A M
authors.initials: PA
authors.lastname: van Leeuwen
authors.forename: Chris J J
authors.initials: CJ
authors.lastname: Mulder
authors.forename: Ad A
authors.initials: AA
authors.lastname: van Bodegraven
mesh_terms: D000042:Absorption; D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D002446:Celiac Disease / Q000097:blood* / Q000503:physiopathology; D002851:Chromatography, High Pressure Liquid; D002956:Citrulline / Q000097:blood*; D005215:Fasting; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D012778:Short Bowel Syndrome / Q000097:blood* / Q000503:physiopathology
chemical_list: D015415:Biomarkers; D002956:Citrulline