title: Separation of floridoside and isofloridosides by HPLC and complete (1)H and (13)C NMR spectral assignments for D-isofloridoside.
abstract: Isofloridosides (1-O-alpha-D-galactopyranosylglycerol) and floridoside (2-O-alpha-D-galactopyranosylglycerol) were extracted from the red alga Porphyra umbilicalis (Linné) Kützing (Bangiales, Rhodophyta). Their separation was achieved by HPLC (NH(2) P50 column) after successive purification of the crude extract by ion-exchange chromatography and HPLC (Sugar-Pak TM1 column). 1D and 2D NMR spectroscopy experiments allowed to completely assign the (1)H and (13)C spectra of D-isofloridoside.
authors.affiliation: Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, Technopôle de Brest-Iroise, F-29280 Plouzané, France. stephanie.bondu@univ-brest.fr
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Bondu
authors.forename: Nelly
authors.initials: N
authors.lastname: Kervarec
authors.forename: Eric
authors.initials: E
authors.lastname: Deslandes
authors.forename: Roger
authors.initials: R
authors.lastname: Pichon
mesh_terms: D002247:Carbon Isotopes; D002851:Chromatography, High Pressure Liquid; D005697:Galactosides / Q000737:chemistry*; D005990:Glycerol / Q000031:analogs & derivatives* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D011522:Protons
chemical_list: C457479:2-galactopyranosylglycerol; D002247:Carbon Isotopes; D005697:Galactosides; D011522:Protons; C025100:isofloridoside; D005990:Glycerol