title: Preparative isolation and purification of chemical components from Aconitum coreanum by high-speed counter-current chromatography coupled with evaporative light scattering detection.
abstract: Aconitum coreanum (Lèvl.) Rapaics (Guanbaifu in Chinese) is a widely used, centuries-old Chinese herb. A preparative high-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) method was employed for isolation and purification of alkaloids from the crude extract of Aconitum coreanum (Lèvl.) Rapaics using ethyl acetate-n-butanol-methanol-0.2 m HCl (7:2:2:7, v/v) as a two-phase solvent system. Six alkaloids, including GFO, GFQ, GFZ, hetisinone, hetisine and GFAA, were obtained in one-step separation. The purity of these compounds was 97.6, 93.8, 91.8, 91.9, 96.2 and 91.1%, respectively.
authors.affiliation: Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing, People's Republic of China.
authors.forename: Qingfa
authors.initials: Q
authors.lastname: Tang
authors.forename: Chunhua
authors.initials: C
authors.lastname: Yang
authors.forename: Wencai
authors.initials: W
authors.lastname: Ye
authors.forename: Jinghan
authors.initials: J
authors.lastname: Liu
authors.forename: Shouxun
authors.initials: S
authors.lastname: Zhao
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000470:Alkaloids; D004224:Diterpenes