Influence of ovarian hormones deprivation on gene expression in the lower urinary tract of rats.
OBJECTIVE
Identify the influence of ovarian hormone deprivation in expression genes on the lower urinary tract of rats.


METHODS
This study deals with gene screening on lower urinary tract of rats. Fifty isogenic rats divided in two groups of twenty-five animals have their lower urinary tract surgically removed: group I, ovariectomized rats 30 days prior to surgery; group II, non-ovariectomized rats. Total RNA was isolated from bladder and urethra, and differential expression of genes was analyzed quantitative, qualitative and comparatively by array technology and RT-PCR.


RESULTS
A total of 76 candidate genes were identified as differentially expressed between the groups, 26 being lower expressed in group II, and 50 in group I. Among them, differential expression validation was confirmed by RT-PCR for three lower expressed genes in group I: Vascular Endothelial Growth Factor (VEGF), Beta-2 Microglobulin (B2M) and Cytochrome c Oxidase subunit I (COX I).


CONCLUSIONS
Ovarian hormone deprivation influences the expression genes on lower urinary tract. We demonstrated that a 30-day period of castration down regulate the expression of VEGF, B2M and COX I in adult rats which are involved in activities of angiogenesis, immune responses and cellular metabolism respectively.
Bortolini
Maria A T
MA
Department of Gynecology, Federal University of Sao Paulo, Sao Paulo, SP, Brazil. maria.augusta@gmail.com
Silva
Ismael D C
ID
Hamerski
Maria G
MG
Castro
Rodrigo A
RA
Sartori
Marair G F
MG
Girao
Manoel J B
MJ
D000818:Animals; D051545:Cyclooxygenase 1 / Q000235:genetics; D004195:Disease Models, Animal; D004967:Estrogens / Q000172:deficiency* / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014551:Urinary Tract / Q000187:drug effects / Q000378:metabolism*; D014570:Urologic Diseases / Q000235:genetics* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000235:genetics*; D001613:beta 2-Microglobulin / Q000235:genetics
D004967:Estrogens; D008565:Membrane Proteins; D042461:Vascular Endothelial Growth Factor A; D001613:beta 2-Microglobulin; D051545:Cyclooxygenase 1; C496513:Ptgs1 protein, rat