title: Bienzymatic glucose biosensor based on co-immobilization of peroxidase and glucose oxidase on a carbon nanotubes electrode.
abstract: A bienzymatic glucose biosensor was proposed for selective and sensitive detection of glucose. This mediatorless biosensor was made by simultaneous immobilization of glucose oxidase (GOD) and horseradish peroxidase (HRP) in an electropolymerized pyrrole (PPy) film on a single-wall carbon nanotubes (SWNT) coated electrode. The amperometric detection of glucose was assayed by potentiostating the bienzymatic electrode at -0.1 versus Ag/AgCl to reduce the enzymatically produced H(2)O(2) with minimal interference from the coexisting electroactive compounds. The single-wall carbon nanotubes, sandwiched between the enzyme loading polypyrrole (PPy) layer and the conducting substrate (gold electrode), could efficiently promote the direct electron transfer of HRP. Operational characteristics of the bienzymatic sensor, in terms of linear range, detection limit, sensitivity, selectivity and stability, were presented in detail.
authors.affiliation: School of Chemistry, Northeast Normal University, Changchun, 130024 Jilin, China. zhuliande@yahoo.com.cn <zhuliande@yahoo.com.cn>
authors.forename: Liande
authors.initials: L
authors.lastname: Zhu
authors.forename: Ruilan
authors.initials: R
authors.lastname: Yang
authors.forename: Jiangli
authors.initials: J
authors.lastname: Zhai
authors.forename: Chunyuan
authors.initials: C
authors.lastname: Tian
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D005947:Glucose