Measurement of interaction force between nanoarrayed integrin alphavbeta3 and immobilized vitronectin on the cantilever tip.
Protein nanoarrays containing integrin alphavbeta3 or BSA were fabricated on ProLinker-coated Au surface by dip-pen nanolithography (DPN). An atomic force microscope (AFM) tip coated with ProLinker was modified by vitronectin. We measured the interaction force between nanoarrayed integrin alphavbeta3 or BSA and immobilized vitronectin on the cantilever tip by employing tethering-unbinding method. The unbinding force between integrin alphavbeta3 and vitronectin (1087+/-62 pN) was much higher than that of between BSA and vitronectin (643+/-74 pN). These results demonstrate that one can distinguish a specific protein interaction from non-specific interactions by means of force measurement on the molecular interactions between the nanoarrayed protein and its interacting protein on the AFM tip.
Lee
Minsu
M
Chungbuk BIT Research-Oriented University Consortium, Chungbuk National University, Cheongju 361-763, South Korea.
Yang
Hyun-Kyu
HK
Park
Keun-Hyung
KH
Kang
Dong-Ku
DK
Chang
Soo-Ik
SI
Kang
In-Cheol
IC
D000327:Adsorption; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D039302:Integrin alphaVbeta3 / Q000737:chemistry* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force / Q000295:instrumentation* / Q000379:methods; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000295:instrumentation* / Q000379:methods; D013314:Stress, Mechanical; D013499:Surface Properties; D014159:Transducers; D019096:Vitronectin / Q000737:chemistry* / Q000648:ultrastructure
D020099:Coated Materials, Biocompatible; D039302:Integrin alphaVbeta3; D019096:Vitronectin