Separation of floridoside and isofloridosides by HPLC and complete (1)H and (13)C NMR spectral assignments for D-isofloridoside.
Isofloridosides (1-O-alpha-D-galactopyranosylglycerol) and floridoside (2-O-alpha-D-galactopyranosylglycerol) were extracted from the red alga Porphyra umbilicalis (Linné) Kützing (Bangiales, Rhodophyta). Their separation was achieved by HPLC (NH(2) P50 column) after successive purification of the crude extract by ion-exchange chromatography and HPLC (Sugar-Pak TM1 column). 1D and 2D NMR spectroscopy experiments allowed to completely assign the (1)H and (13)C spectra of D-isofloridoside.
Bondu
Stéphanie
S
Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, Technopôle de Brest-Iroise, F-29280 Plouzané, France. stephanie.bondu@univ-brest.fr
Kervarec
Nelly
N
Deslandes
Eric
E
Pichon
Roger
R
D002247:Carbon Isotopes; D002851:Chromatography, High Pressure Liquid; D005697:Galactosides / Q000737:chemistry*; D005990:Glycerol / Q000031:analogs & derivatives* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D011522:Protons
C457479:2-galactopyranosylglycerol; D002247:Carbon Isotopes; D005697:Galactosides; D011522:Protons; C025100:isofloridoside; D005990:Glycerol