Factor XI/ADAMTS13 complexes are quantitatively insignificant in human plasma.
Reportedly, complexes between factor XI and ADAMTS13 are detected with a commercial ADAMTS13/FXI ELISA kit in plasma and are decreased in thrombotic thrombocytopenic purpura (TTP). Using this kit, control and TTP patient plasma contained varying amounts of signal (25-670% of a reference plasma) but no signal was observed for mixtures of recombinant enzymes, suggesting little interaction. ADAMTS13/FXI complexes were undetectable by immunoprecipitation or gel filtration chromatography in control plasma or mixtures of recombinant proteins. These results suggest that ADAMTS13/FXI complexes are insignificant in plasma and unlikely to affect the function of either protein during normal hemostasis or in TTP.
Anderson
Patricia J
PJ
Department of Medicine, Washington University School of Medicine, 660 S. Euclid, Box 8066, St. Louis, MO 63110, USA. tanderso@im.wustl.edu
Gailani
David
D
Feys
Hendrik B
HB
Gao
Weiqiang
W
Majerus
Elaine M
EM
Vanhoorelbeke
Karen
K
Sadler
J Evan
JE
D051722:ADAM Proteins / Q000097:blood*; D000071120:ADAMTS13 Protein; D002850:Chromatography, Gel; D005172:Factor XI / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding
D005172:Factor XI; D051722:ADAM Proteins; D000071120:ADAMTS13 Protein; C099604:ADAMTS13 protein, human