title: Characterization of alpha-amylase inhibitor from Palo Fierro seeds.
abstract: Alpha amylase inhibitor from Palo Fierro seeds (alphaAI-PF) was purified using affinity chromatography on a fetuin-fractogel column followed by anionic exchange chromatography. AlphaAI-PF has a molecular mass of 77kDa with two subunits (15.8 and 17.4 kDa), it is nonglycosylated and has pI of 4.7. AlphaAI-PF inhibited porcine pancreatic alpha-amylase (PPA) (1,4-alpha-D-glucan glucanohydrolase; EC 3.2.1.1), but was almost devoid of inhibitory activity on alpha-amylase extracts from Zabrotes subfasciatus (ZSA). Analysis of alphaAI-PF peptides showed a high homology to alphaAI-1 from Phaseolus vulgaris that also inhibits PPA.
authors.affiliation: Centro de Investigacion en Alimentacion y Desarrollo, A.C., Ciencia de los Alimentos, Apartado Postal 1735, 83000 Hermosillo, Sonora, Mexico.
authors.forename: A M
authors.initials: AM
authors.lastname: Guzman-Partida
authors.forename: O
authors.initials: O
authors.lastname: Jatomea-Fino
authors.forename: M R
authors.initials: MR
authors.lastname: Robles-Burgueño
authors.forename: M
authors.initials: M
authors.lastname: Ortega-Nieblas
authors.forename: L
authors.initials: L
authors.lastname: Vazquez-Moreno
mesh_terms: D000595:Amino Acid Sequence; D004305:Dose-Response Relationship, Drug; D007887:Fabaceae / Q000737:chemistry*; D008969:Molecular Sequence Data; D012639:Seeds / Q000737:chemistry*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
chemical_list: D000516:alpha-Amylases