title: CE of phytosiderophores and related metal species in plants.
abstract: Phytosiderophores (PS) and the closely related substance nicotianamine (NA) are key substances in metal uptake into graminaceous plants. Here, the CE separation of these substances and related metal species is demonstrated. In particular, the three PS 2'-deoxymugineic acid (DMA), mugineic acid (MA), and 3-epi-hydroxymugineic acid (epi-HMA), and NA, are separated using MES/Tris buffer at pH 7.3. Moreover, three Fe(III) species of the different PS are separated without any stability problems, which are often present in chromatographic analyses. Also divalent metal species of Cu, Ni, and Zn with the ligands DMA and NA are separated with the same method. By using a special, zwitterionic CE capillary, even the separation of two isomeric Fe(III) chelates with the ligand ethylenediamine-N,N'-bis(o-hydroxyphenyl)acetic acid (EDDHA) is possible (i.e., meso-Fe(III)-EDDHA and rac-Fe(III)-EDDHA), and for fast separations of NA and respective divalent and trivalent metal species, a polymer CE microchip with suppressed EOF is described. The proposed CE method is applicable to real plant samples, and enables to detect changes of metal species (Cu-DMA, Ni-NA), which are directly correlated to biological processes.
authors.affiliation: Institute for Analytical Sciences, Bunsen-Kirchoff-Strasse 11, D-44139 Dortmund, Germany.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xuan
authors.forename: Enrico B
authors.initials: EB
authors.lastname: Scheuermann
authors.forename: Anderson R
authors.initials: AR
authors.lastname: Meda
authors.forename: Peter
authors.initials: P
authors.lastname: Jacob
authors.forename: Nicolaus
authors.initials: N
authors.lastname: von Wirén
authors.forename: Günther
authors.initials: G
authors.lastname: Weber
mesh_terms: D001383:Azetidinecarboxylic Acid / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D002021:Buffers; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods; D005029:Ethylenediamines; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000032:analysis*; D007502:Iron Chelating Agents / Q000032:analysis* / Q000737:chemistry; D008670:Metals / Q000145:classification; D010944:Plants / Q000737:chemistry*; D012680:Sensitivity and Specificity; D017262:Siderophores / Q000032:analysis* / Q000737:chemistry; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002021:Buffers; D005029:Ethylenediamines; D007502:Iron Chelating Agents; D008670:Metals; D017262:Siderophores; C011813:ethylenediamine-N,N'-bis(2-hydroxyphenylacetic acid); D001383:Azetidinecarboxylic Acid; D007501:Iron; C052863:mugineic acid