Methylophaga aminisulfidivorans sp. nov., a restricted facultatively methylotrophic marine bacterium.
A novel restricted facultatively methylotrophic marine strain, MP(T), possessing the ribulose monophosphate pathway of C(1)-carbon compound assimilation was isolated from a seawater sample obtained from Mokpo, South Korea. The novel isolate is aerobic, Gram-negative, asporogenous and a non-motile short rod. It grows well on methanol, methylated amines, dimethylsulfide and DMSO. Optimal growth occurs with 3 % NaCl at 30 degrees C and pH 7.0. Fructose is utilized as a multicarbon source. Growth factors are not required and vitamin B(12) does not stimulate growth. The cellular fatty acid profile of the novel strain consists primarily of straight-chain saturated C(16 : 0) and unsaturated C(16 : 1) acids. The major ubiquinone is Q-8. The dominant phospholipids are phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content is 44.9 mol% (T(m)). Based on 16S rRNA gene sequence analysis and DNA-DNA relatedness (25-41 %) with the type strains of marine methylotrophs belonging to the genus Methylophaga, it is suggested that isolate MP(T) represents a novel species, Methylophaga aminisulfidivorans sp. nov. (type strain MP(T)=KCTC 12909(T)=VKM B-2441(T)=JCM 14647(T)).
Kim
Hee Gon
HG
Department of Biomaterials Engineering, Chosun University, Gwangju 501-759, South Korea.
Doronina
Nina V
NV
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia.
Trotsenko
Yuri A
YA
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia.
Kim
Si Wouk
SW
Department of Environmental Engineering, Chosun University, Gwangju 501-759, South Korea.
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002244:Carbon / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004121:Dimethyl Sulfoxide / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D005632:Fructose / Q000378:metabolism; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D007723:Korea; D000432:Methanol / Q000378:metabolism; D008744:Methylamines / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D044147:Piscirickettsiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D013440:Sulfides / Q000378:metabolism; D013696:Temperature; D014451:Ubiquinone / Q000032:analysis; D014805:Vitamin B 12 / Q000378:metabolism
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D008744:Methylamines; D010743:Phospholipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013440:Sulfides; D014451:Ubiquinone; D005632:Fructose; D012965:Sodium Chloride; D002244:Carbon; D014805:Vitamin B 12; C004784:dimethyl sulfide; D000432:Methanol; D004121:Dimethyl Sulfoxide