title: Determination of domoic acid toxins in shellfish by biosense ASP ELISA--a direct competitive enzyme-linked immunosorbent assay: collaborative study.
abstract: A collaborative study was conducted on the Biosense amnesic shellfish poisoning (ASP) enzyme-linked immunosorbent assay (ELISA) for the determination of domoic acid (DA) toxins in shellfish in order to obtain interlaboratory validation data for the method. In addition, a method comparison study was performed to evaluate the ASP ELISA as an alternative to the current liquid chromatography (LC) reference method for DA determination. The study material comprised 16 shellfish samples, including blue mussels, Pacific oysters, and king scallops, spiked with contaminated mussel homogenates to contain 0.1-20 mg DA/kg shellfish flesh. The shellfish samples were extracted with 50% aqueous methanol, and the supernatants were directly analyzed. Sixteen participating laboratories in 10 countries reported data from the ASP ELISA, and 4 of these laboratories also reported data from instrumental LC analysis. The participating laboratories achieved interlaboratory precision estimates for the 8 Youden paired shellfish samples in the range of 10-20% for RSD(r) (mean 14.8 +/- 4%), and 13-29% for RSDR (mean 22.7 +/- 6%). The precision estimates for the ELISA data did not show a strong dependence on the DA concentration in the study samples, and the overall precision achieved was within the acceptable range of the Horwitz guideline with HorRat values ranging from 1.1 to 2.4 (mean HorRat 1.7 +/- 0.5). The analysis of shellfish samples spiked with certified reference material (CRM)-ASP-MUS-b gave recoveries in the range of 88-122%, with an average recovery of 104 +/- 10%. The estimate on method accuracy was supported by a correlation slope of 1.015 (R2 = 0.992) for the determined versus the expected DA values. Furthermore, the correlation of the ASP ELISA results with those for the instrumental LC analyses of the same sample extracts gave a correlation slope of 1.29 (R2 = 0.984). This indicates some overestimation of DA levels in shellfish by the ELISA, but it is also a result of apparent low recoveries for the LC methods. This interlaboratory study demonstrates that the ASP ELISA is suitable for the routine determination and monitoring of DA toxins in shellfish, and that it offers a rapid and cost-effective methodology with high sample throughput.
authors.affiliation: Biosense Laboratories AS, HIB-Thormøhlensgate 55, NO-5008 Bergen, Norway. hans.kleivdal@biosense.com
authors.forename: Hans
authors.initials: H
authors.lastname: Kleivdal
authors.forename: Sven-Inge
authors.initials: SI
authors.lastname: Kristiansen
authors.forename: Mona V
authors.initials: MV
authors.lastname: Nilsen
authors.forename: Anders
authors.initials: A
authors.lastname: Goksøyr
authors.forename: Lyn
authors.initials: L
authors.lastname: Briggs
authors.forename: Patrick
authors.initials: P
authors.lastname: Holland
authors.forename: Paul
authors.initials: P
authors.lastname: McNabb
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D049872:Bivalvia / Q000378:metabolism; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis; D005506:Food Contamination; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D008387:Marine Toxins / Q000737:chemistry*; D049897:Pectinidae / Q000378:metabolism; D015203:Reproducibility of Results; D012758:Shellfish
chemical_list: D008387:Marine Toxins; C012301:domoic acid; D007608:Kainic Acid