title: Crystallization and preliminary X-ray diffraction studies of choline-binding protein F from Streptococcus pneumoniae.
abstract: Choline-binding protein F (CbpF) is a modular protein that is bound to the pneumococcal cell wall through noncovalent interactions with choline moieties of the bacterial teichoic and lipoteichoic acids. Despite being one of the more abundant proteins on the surface, along with the murein hydrolases LytA, LytB, LytC and Pce, its function is still unknown. CbpF has been crystallized using the hanging-drop vapour-diffusion method at 291 K. Diffraction-quality orthorhombic crystals belong to space group P2(1)2(1)2, with unit-cell parameters a = 49.13, b = 114.94, c = 75.69 A. A SAD data set from a Gd-HPDO3A-derivatized CbpF crystal was collected to 2.1 A resolution at the gadolinium L(III) absorption edge using synchrotron radiation.
authors.affiliation: Grupo de Cristalografía Macromolecular y Biología Estructural, Instituto Química Física Rocasolano, CSIC, Serrano 119, 28006 Madrid, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Molina
authors.forename: Ana
authors.initials: A
authors.lastname: González
authors.forename: Miriam
authors.initials: M
authors.lastname: Moscoso
authors.forename: Pedro
authors.initials: P
authors.lastname: García
authors.forename: Meike
authors.initials: M
authors.lastname: Stelter
authors.forename: Richard
authors.initials: R
authors.lastname: Kahn
authors.forename: Juan A
authors.initials: JA
authors.lastname: Hermoso
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D013296:Streptococcus pneumoniae / Q000737:chemistry*; D017356:Synchrotrons; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D011994:Recombinant Proteins