Marinobacter segnicrescens sp. nov., a moderate halophile isolated from benthic sediment of the South China Sea.
A Gram-negative, motile, non-spore-forming and moderately halophilic ellipsoid-shaped marine coccobacillus, designated strain SS011B1-4(T), was isolated from benthic sediment of the South China Sea. Optimum growth occurred at 30-37 degrees C, pH 7.5-8.0 and 4-8 % (w/v) NaCl. Strain SS011B1-4(T) utilized a variety of organic substrates as sole carbon sources, but did not utilize toluene, n-tetradecane or crude oil. Strain SS011B1-4(T) had ubiquinone-9 as the major respiratory quinone and C(18 : 1)omega9c, C(16 : 0) and C(12 : 0) 3-OH as the predominant fatty acids. The genomic DNA G+C content was 62.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SS011B1-4(T) belonged to the genus Marinobacter of the Gammaproteobacteria. The results of the phenotypic, phylogenetic and genomic analyses revealed that strain SS011B1-4(T) represents a novel species of the genus Marinobacter. The name Marinobacter segnicrescens sp. nov. is therefore proposed, with strain SS011B1-4(T) (=LMG 23928(T)=CGMCC 1.6489(T)) as the type strain.
Guo
Bin
B
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, P. R. China.
Gu
Jun
J
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, P. R. China.
Ye
Yu-Guang
YG
Qingdao Institute of Marine Geology, Qingdao 266071, P. R. China.
Tang
Yue-Qin
YQ
Department of Materials and Life Science, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto City, Kumamoto 860-8555, Japan.
Kida
Kenji
K
Department of Materials and Life Science, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto City, Kumamoto 860-8555, Japan.
Wu
Xiao-Lei
XL
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, P. R. China.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D008124:Locomotion / Q000502:physiology; D053520:Marinobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009930:Organic Chemicals / Q000378:metabolism; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012462:Saline Solution, Hypertonic / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013171:Spores, Bacterial / Q000166:cytology; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009930:Organic Chemicals; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012462:Saline Solution, Hypertonic