Flavobacterium filum sp. nov., isolated from a wastewater treatment plant in Korea.
A Gram-negative bacterium, designated strain EMB34(T), was isolated from a wastewater treatment plant in Korea. Growth was observed between 10 and 40 degrees C (optimum, 25-35 degrees C) and between pH 6.0 and 9.5 (optimum, pH 7.5-8.0). The cells were non-motile rods, linked with extracellular fibrils. The predominant fatty acids of strain EMB34(T) were iso-C(15 : 0), C(15 : 0), iso-C(15 : 1) G, iso-C(16 : 0) 3-OH and iso-C(15 : 0) 3-OH and the strain contained phosphatidylethanolamine and phosphatidylinositol as the polar lipids. The G+C content of the genomic DNA was 34.2 mol% and the major quinone was menaquinone-6. Comparative 16S rRNA gene sequence analyses showed that strain EMB34(T) formed a distinct phyletic line within the genus Flavobacterium. The levels of 16S rRNA gene sequence similarity with other Flavobacterium species were less than 94.5 %. On the basis of phenotypic, chemotaxonomic and molecular data, it is clear that strain EMB34(T) represents a novel species within the genus Flavobacterium, for which the name Flavobacterium filum sp. nov. is proposed. The type strain is EMB34(T) (=KCTC 12610(T)=DSM 17961(T)).
Ryu
Seung Hyun
SH
Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Park
Minjeong
M
Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Jeon
Yeji
Y
Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Lee
Jung Ro
JR
Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Park
Woojun
W
Division of Environmental Science and Ecological Engineering, Korea University, Seoul 136-701, Republic of Korea.
Jeon
Che Ok
CO
Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature; D014871:Water Microbiology; D018508:Water Purification
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S