title: Cell culture models of higher complexity in tissue engineering and regenerative medicine.
abstract: Cell culture techniques have tended to be used in biomaterial research as a screening method prior to embarking on specific in vivo experimentation. This presentation aims at showing that it is possible to develop more sophisticated in vitro systems using primary human cells in co-culture with other cell types and biomaterials in a three-dimensional setting. While the predictive value of such systems is still not proven these models can be employed to unravel the complexity of biological systems in order to understand molecular mechanisms of cell-cell and cell-material interactions. The brief overview is under the headings of basic principles of relevant culture systems, the study of inflammation and the healing response, scenarios for specific biomaterial applications and future directions. How human endothelial cells can be usefully incorporated into more complex cell culture models is presented as an example of how relevant questions in tissue engineering and regenerative medicine can be addressed. The central tenet of this paper is that it is possible to refine in vitro methodology using cells of human origin to establish relevant assay systems that more closely simulate the cellular and molecular microenvironment encountered in a specific situation of regeneration using biomaterials.
authors.affiliation: REPAIR-lab, Institute of Pathology, Johannes Gutenberg University, D-55101 Mainz, Germany. kirkpatrick@repair-lab.org
authors.forename: Charles
authors.initials: C
authors.lastname: James Kirkpatrick
authors.forename: Sabine
authors.initials: S
authors.lastname: Fuchs
authors.forename: Maria
authors.initials: M
authors.lastname: Iris Hermanns
authors.forename: Kirsten
authors.initials: K
authors.lastname: Peters
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Unger
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001698:Biomedical Engineering / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods*; D018920:Coculture Techniques / Q000379:methods; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D007249:Inflammation; D036103:Nanotechnology / Q000379:methods; D012038:Regeneration; D044968:Regenerative Medicine / Q000379:methods*; D046509:Tissue Culture Techniques; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials