Monitoring neutrophil engraftment in allogeneic stem cell transplantation by flow cytometric analysis of neutrophil-specific antigens NA1 and NA2.
Neutrophil-specific antigen (NA) expression on neutrophils was analysed in 18 Japanese children before and after allogeneic stem cell transplantation (allo-SCT) with myeloablative regimen. Donor-recipient NA-incompatibility was present in one of eight NA1/NA2 heterozygous patients and eight of 10 NA1/NA1 or NA2/NA2 homozygous patients. After allo-SCTs from NA-incompatible donors, a neutrophil recipient-to-donor conversion was confirmed in all cases. Conversion to donor NA type was complete before the absolute neutrophil count reached 0.1 x 10(9)/l. These observations indicate that flow cytometric analysis of NA antigens is a simple and useful method for monitoring neutrophil engraftment in NA-incompatible allo-SCT.
Inukai
Takeshi
T
Department of Paediatrics, School of Medicine, University of Yamanashi, Yamanashi, Japan. tinukai@yamanashi.ac.jp
Uno
Kanako
K
Taniguchi
Kikuyo
K
Goi
Kumiko
K
Tezuka
Toru
T
Nemoto
Atsushi
A
Takahashi
Kazuya
K
Sato
Hiroki
H
Akahane
Koshi
K
Hirose
Kinuko
K
Honna
Hiroko
H
Kagami
Keiko
K
Hiraoka
Asako
A
Tanihiro
Misae
M
Nakazawa
Shinpei
S
Sugita
Kanji
K
D000911:Antibodies, Monoclonal / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D002648:Child; D005434:Flow Cytometry; D006085:Graft Survival; D006801:Humans; D007519:Isoantigens / Q000032:analysis*; D009504:Neutrophils / Q000378:metabolism / Q000637:transplantation*; D033581:Stem Cell Transplantation / Q000379:methods*; D013995:Time; D014184:Transplantation, Homologous
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D007519:Isoantigens; C067704:neutrophil-specific antigen NA1, human; C072753:neutrophil-specific antigen NA2