A new hippocampal model for examining intracerebral hemorrhage-related neuronal death: effects of deferoxamine on hemoglobin-induced neuronal death.
OBJECTIVE
There is an urgent need to develop a model in which to study the mechanism of intracerebral hemorrhage-induced neuronal death in vivo.


METHODS
This study was divided into 2 parts: (1) Rats received either an infusion of hemoglobin, ferrous iron, or saline into the right hippocampus; (2) Rats had an infusion of hemoglobin and then were treated with either deferoxamine or vehicle. Rats were killed for hippocampus size, DNA damage, and neuronal death measurements.


RESULTS
Compared with saline, hemoglobin or iron injection caused hippocampal neuronal death. Systemic use of deferoxamine reduced hemoglobin-induced DNA damage, hippocampal neuronal death, and atrophy.


CONCLUSIONS
This article demonstrates a new model and indicates that iron has a key role in hemoglobin-induced neuronal death.
Song
Shuijiang
S
Hua
Ya
Y
Keep
Richard F
RF
Hoff
Julian T
JT
Xi
Guohua
G
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002543:Cerebral Hemorrhage / Q000188:drug therapy* / Q000473:pathology; D003676:Deferoxamine / Q000494:pharmacology*; D004195:Disease Models, Animal; D006454:Hemoglobins / Q000633:toxicity; D006624:Hippocampus / Q000473:pathology; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017262:Siderophores / Q000494:pharmacology*
D006454:Hemoglobins; D017262:Siderophores; D003676:Deferoxamine