Wound dressings based on chitosans and hyaluronic acid for the release of chlorhexidine diacetate in skin ulcer therapy.
In the present work wound dressings, based on chitosan hydrochloride (HCS), 5-methyl-pyrrolidinone chitosan (MPC), and their mixtures with an anionic polymer, hyaluronic acid (HA), were prepared by freeze-drying. Chlorhexidine diacetate (CX) was used as an antimicrobic drug. The mechanical properties of the wound dressings were investigated. In particular, the wound dressings were subjected to dynamic hydration measurements to evaluate their capability to absorb wound exudate and to rheological analysis to investigate their resistance to mechanical stresses on hydration. The wound dressings were also characterized for drug release properties. The antioxidant and antimicrobial activities of medicated and non-medicated wound dressings were also investigated. All the wound dressings are characterized by mechanical resistance suitable for skin application. The addition of hyaluronic acid to chitosans leads to a reduction in wound dressing hydration properties and a modulation of drug release. The wound dressing based on MPC is characterized by the highest elastic properties and by the best scavenger activity. Antimicrobial activity against bacteria and C. albicans is shown by the dressing based on chitosan also in absence of chlorhexidine.
Rossi
Silvia
S
Department of Pharmaceutical Chemistry, University of Pavia, Viale Taramelli, Pavia, Italy.
Marciello
Marzia
M
Sandri
Giuseppina
G
Ferrari
Franca
F
Bonferoni
Maria Cristina
MC
Papetti
Adele
A
Caramella
Carla
C
Dacarro
Casare
C
Grisoli
Pietro
P
D000287:Administration, Topical; D000891:Anti-Infective Agents, Local / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D001458:Bandages; D048271:Chitosan / Q000737:chemistry; D002710:Chlorhexidine / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D004548:Elasticity; D005609:Free Radicals / Q000378:metabolism; D005612:Freeze Drying; D006820:Hyaluronic Acid / Q000737:chemistry; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D011760:Pyrrolidinones / Q000737:chemistry; D012212:Rheology; D012883:Skin Ulcer; D014783:Viscosity; D014945:Wound Healing; D014946:Wound Infection
D000891:Anti-Infective Agents, Local; D000975:Antioxidants; D004337:Drug Carriers; D005609:Free Radicals; D011760:Pyrrolidinones; C085060:methylpyrrolidinone chitosan; D006820:Hyaluronic Acid; D048271:Chitosan; D002710:Chlorhexidine