title: Validated liquid chromatographic method for quantitative determination of allicin in garlic powder and tablets.
abstract: In the present study, an RP high performance liquid chromatographic method was developed and validated for the determination of allicin in garlic powder and tablets. Chromatographic separation was carried out on an RP-18(e )column (125 mm x 4 mm), using a mobile phase, consisting of methanol-water (50:50 v/v), at a flow rate of 0.5 mL/min and UV detection at 220 nm. Ethylparaben was used as the internal standard. The assay was linear for allicin concentrations of 5.0-60.0 microg/mL. The RSD for precision was <6.14%. The accuracy was above 89.11%. The detection and quantification limits were 0.27 and 0.81 microg/mL, respectively. This method was used to quantify allicin in garlic powder samples. The results showed that the method described here is useful for the determination of allicin in garlic powder and tablets.
authors.affiliation: Pharmacy Department, Pharmacy School, University of Concepción, Concepción, Chile. mdediego@udec.cl
authors.forename: Marta
authors.initials: M
authors.lastname: de Diego
authors.forename: Marcia
authors.initials: M
authors.lastname: Avello
authors.forename: Sigrid
authors.initials: S
authors.lastname: Mennickent
authors.forename: Marcos
authors.initials: M
authors.lastname: Fernández
authors.forename: Pola
authors.initials: P
authors.lastname: Fernández
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004220:Disulfides; D011208:Powders / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013441:Sulfinic Acids / Q000032:analysis*; D013607:Tablets / Q000737:chemistry*
chemical_list: D004220:Disulfides; D011208:Powders; D013441:Sulfinic Acids; D013607:Tablets; C006452:allicin