Pharmacokinetic study of three cardiovascular drugs by high-performance liquid chromatography using pre-column derivatization with 9,10-anthraquinone-2-sulfonyl chloride.
A new method was developed to analyze three cardiovascular drugs in rat plasma, Mexiletine hydrochloride (MXL), Methoxamine hydrochloride (MTX), and Metaraminol bitartrate (MTR), by high-performance liquid chromatography (HPLC) using 9,10-anthraquinone-2-sulfonyl chloride (ASC) as the derivatization reagent. The derivatization modes and conditions for this method were optimized. The quantitative analysis was achieved using a C18 column at room temperature (25 degrees C), with various volume ratios of methanol-water as the mobile phase and a detection wavelength at 256 nm. Analytical linearity was obtained for the method over the concentration range of 0.04-8.0 microg mL(-1) for all the three drugs. The lower limit of quantification (LLOQ) was 0.04 microg mL(-1). This method was successfully applied to the analysis of the three drugs in rat plasma and their pharmacokinetic studies. The t1/2 values of the three drugs in rats were found to be 5.38+/-0.61, 4.49+/-0.53, and 3.70+/-0.19 h for MXL, MTX, and MTR, respectively.
Liu
Han
H
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China.
Feng
Fang
F
Ma
Ming
M
Cui
Shuangjin
S
Xie
Darong
D
Xu
Sizhi
S
D000818:Animals; D000880:Anthraquinones / Q000737:chemistry*; D002317:Cardiovascular Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006207:Half-Life; D008297:Male; D008680:Metaraminol / Q000097:blood / Q000493:pharmacokinetics; D008729:Methoxamine / Q000097:blood / Q000493:pharmacokinetics; D008801:Mexiletine / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013441:Sulfinic Acids / Q000737:chemistry*
D000880:Anthraquinones; D002317:Cardiovascular Agents; D013441:Sulfinic Acids; C042834:9,10-anthraquinone; D008801:Mexiletine; C044255:sulfonyl chloride; D008680:Metaraminol; D008729:Methoxamine