Differential expression of microRNA species in human uterine leiomyoma versus normal myometrium.
OBJECTIVE
To determine whether microRNAs (miRNAs) are differentially expressed in human leiomyoma versus matched myometrial tissue.


METHODS
Microarray with real-time polymerase chain reaction (PCR) validation.


METHODS
Academic medical center.


METHODS
Premenopausal subjects (n = 15), who were undergoing hysterectomies for leiomyoma-related symptoms.


METHODS
None.


METHODS
Statistically differential expression of miRNAs in leiomyoma versus myometrium.


RESULTS
Forty-six miRNA species were differentially expressed in leiomyoma versus normal myometrium. Of these, 19 were overexpressed and 27 were underexpressed in leiomyomas. The changes ranged from 1.2-fold to 11.8-fold. These findings were confirmed using real-time reverse transcriptase PCR for selected miRNAs (miRNAs 21, 34a, 125b, 139, and 323).


CONCLUSIONS
Our findings indicate that miRNAs are differentially expressed between human leiomyoma and matched myometrium. Given this differential expression, miRNAs may play a role in the pathogenesis of uterine leiomyoma and may serve as future therapeutic targets for the treatment of these tumors.
Marsh
Erica E
EE
Division of Reproductive Biology Research, Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA.
Lin
Zhihong
Z
Yin
Ping
P
Milad
Magdy
M
Chakravarti
Debabrata
D
Bulun
Serdar E
SE
D000328:Adult; D002467:Cell Nucleus / Q000235:genetics; D003593:Cytoplasm / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007044:Hysterectomy; D007889:Leiomyoma / Q000235:genetics* / Q000601:surgery; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009215:Myometrium / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D017697:Premenopause; D012016:Reference Values; D014594:Uterine Neoplasms / Q000235:genetics* / Q000601:surgery
D035683:MicroRNAs