title: Purine nucleobase transport in the intraerythrocytic malaria parasite.
abstract: Hypoxanthine, a nucleobase, serves as the major source of the essential purine group for the intraerythrocytic malaria parasite. In this study we have measured the uptake of hypoxanthine, and that of the related purine nucleobase adenine, by mature blood-stage Plasmodium falciparum parasites isolated from their host cells by saponin-permeabilisation of the erythrocyte and parasitophorous vacuole membranes. The uptake of both [3H]hypoxanthine and [3H]adenine was comprised of at least two components; in each case there was a rapid equilibration of the radiolabel between the intra- and extracellular solutions via a low-affinity transport mechanism, and an accumulation of radiolabel (such that the estimated intracellular concentration exceeded the extracellular concentration) via a higher-affinity process. The uptake of [3H]adenine was studied in more detail. The rapid, low-affinity equilibration of [3H]adenine between the intra-and extracellular solution was independent of the energy status of the parasite whereas the higher-affinity accumulation of the radiolabel was ATP-dependent. A kinetic analysis of adenine uptake revealed that the low-affinity (equilibrative) process had a Km of approximately 1.2mM, similar to the value of 0.82 mM estimated here (using the Xenopus laevis oocyte expression system) for the Km for the transport of adenine by PfENT1, a parasite-encoded member of the 'equilibrative nucleoside/nucleobase transporter' family. The results indicate that nucleobases enter the intraerythrocytic parasite via a rapid, equilibrative process that has kinetic characteristics similar to those of PfENT1.
authors.affiliation: School of Biochemistry and Molecular Biology, Faculty of Science, The Australian National University, Canberra, ACT 0200, Australia.
authors.forename: Megan J
authors.initials: MJ
authors.lastname: Downie
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Saliba
authors.forename: Stefan
authors.initials: S
authors.lastname: Bröer
authors.forename: Susan M
authors.initials: SM
authors.lastname: Howitt
authors.forename: Kiaran
authors.initials: K
authors.lastname: Kirk
mesh_terms: D000225:Adenine / Q000032:analysis / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D004912:Erythrocytes / Q000469:parasitology*; D019271:Hypoxanthine / Q000032:analysis / Q000378:metabolism; D016778:Malaria, Falciparum; D033702:Nucleobase Transport Proteins / Q000032:analysis / Q000378:metabolism*; D033701:Nucleobase, Nucleoside, Nucleotide, and Nucleic Acid Transport Proteins / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism; D010274:Parasitology / Q000379:methods; D010963:Plasmodium falciparum / Q000378:metabolism*; D015800:Protozoan Proteins / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: C430711:ENT1 protein, Plasmodium falciparum; D033702:Nucleobase Transport Proteins; D033701:Nucleobase, Nucleoside, Nucleotide, and Nucleic Acid Transport Proteins; D015800:Protozoan Proteins; D019271:Hypoxanthine; D000255:Adenosine Triphosphate; D000225:Adenine