title: Bioassay-guided isolation of kaempferol-3-O-beta-D-galactoside with anti-inflammatory and antinociceptive activity from the aerial part of Calluna vulgaris L.
abstract: Calluna vulgaris L. (Ericaceae) is used for the treatment of various inflammatory ailments in traditional medicines. In order to evaluate this ethnobotanical information, its anti-inflammatory and antinociceptive activities were studied using in vivo experimental models in mice. The ethanolic extract of the plant was first fractionated into five extracts; namely, n-hexane, chloroform, ethyl acetate (EtOAc), n-butanol, and water fractions. Among them, the EtOAc Fr. was found to be the most effective and was further subjected to bioassay-guided fractionation and isolation procedures. After successive column chromatography applications, on Sephadex LH-20 and silica gel, a component, which is responsible for the above-mentioned activities of this species of Turkish origin, was isolated and its structure was elucidated as kaempferol-3-O-beta-D-galactoside, a common flavonol derivative by means of spectral techniques.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Orhan
authors.forename: E
authors.initials: E
authors.lastname: Küpeli
authors.forename: S
authors.initials: S
authors.lastname: Terzioğlu
authors.forename: E
authors.initials: E
authors.lastname: Yesilada
mesh_terms: D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D016227:Benzoquinones; D001681:Biological Assay; D029795:Calluna / Q000737:chemistry*; D002351:Carrageenan; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D044949:Kaempferols / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D008297:Male; D051379:Mice; D009005:Monosaccharides / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D010146:Pain / Q000188:drug therapy; D010147:Pain Measurement; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D012997:Solvents / Q000737:chemistry; D023382:Toxicity Tests, Acute; D014421:Turkey
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D016227:Benzoquinones; D044949:Kaempferols; D009005:Monosaccharides; D010936:Plant Extracts; D012997:Solvents; C526104:kaempferol-3-O-beta-D-galactoside; C004532:quinone; D002351:Carrageenan