title: Development of an eco-friendly agar extraction technique from the red seaweed Gracilaria lemaneiformis.
abstract: The red seaweed, Gracilaria lemaneiformis growing as an aquaculture bioremediator along the coasts of Liaodong Peninsula, China, was investigated for the agar production. An eco-friendly method called agar photobleaching extraction process was developed for the benefit of workers' health and safety of the environment. The native agar (NA), alkali-modified agar (AA), chemical-bleached agar (CA) and photobleached agar (PA), which were extracted using different processes, were evaluated for their physical and chemical properties. The PA showed most desirable performances in terms of gel strength, gelling temperature, sulfate content and 3,6-anhydro-l-galactose content. Among the different processed agars, PA gel strength was 1913 g/cm2, the highest among the different processed agars, which increased 8.6% on the basis of the AA. Further we applied this new technique to extract agars from Gracilaria asiatica, and similar results were obtained with that of G. lemaneiformis. This indicates that the agar photobleaching extraction process is a feasible method for Gracilaria species and has a potential application. During the whole agar photobleaching extraction process the pigment content of G. lemaneiformis declined gradually and the TOC concentration in photobleaching solution increased along with the increase in the irradiation time. The mechanism of agar photobleaching could be elucidated by the photolysis theory.
authors.affiliation: Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.forename: Xingju
authors.initials: X
authors.lastname: Yu
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yuanling
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000362:Agar / Q000302:isolation & purification* / Q000528:radiation effects; D001673:Biodegradation, Environmental; D002681:China; D017753:Ecosystem; D005690:Galactose / Q000032:analysis; D044724:Gracilaria / Q000737:chemistry* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D008027:Light; D012623:Seawater; D012624:Seaweed / Q000737:chemistry* / Q000302:isolation & purification; D013431:Sulfates / Q000032:analysis
chemical_list: D013431:Sulfates; D000362:Agar; D005690:Galactose