Genotyping spring viraemia of carp virus and other piscine vesiculo-like viruses using reverse hybridisation.
A simple nylon membrane-based DNA macroarray was developed to genotype spring viraemia of carp virus (SVCV) and related viruses. Twenty-six viruses were genotyped using the array, and the results were confirmed by phylogenetic analysis of a 426 bp partial glycoprotein gene sequence. The array was not only capable of discriminating between the 4 main genogroups of cyprinid vesiculo-type viruses described previously, but also accurately sub-type the SVC viruses assigned to Genogroup I. The assay offers a practical solution for diagnostic laboratories that currently lack a sequencing capability to confirm the nature of PCR products generated in suspected SVCV cases.
Sheppard
A M
AM
Centre for Environment, Fisheries and Aquaculture Science, Weymouth Laboratory, Barrack Road, Weymouth, Dorset DT4 8UB, UK.
Le Deuff
R M
RM
Martin
P D
PD
Woolford
G
G
Way
K
K
Stone
D M
DM
D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000821:virology*; D015342:DNA Probes / Q000737:chemistry; D005393:Fish Diseases / Q000821:virology*; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000662:veterinary*; D010802:Phylogeny; D018353:Rhabdoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D012680:Sensitivity and Specificity; D018116:Vesiculovirus / Q000235:genetics*; D014766:Viremia / Q000662:veterinary* / Q000821:virology
D015342:DNA Probes; D006023:Glycoproteins