Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales.
Complementary DNAs encoding two major osteoclastic markers, tartrate-resistant acid phosphatase (TRAP) and cathepsin K (Cath K) were cloned from the scales of a teleost, the goldfish. This is the first report of the full coding sequence of TRAP and Cath K molecules in fish. In the goldfish scale both TRAP and Cath K mRNAs were expressed in the multinucleate osteoclasts, which showed large numbers of mitochondria and lysosomes, and a well developed ruffled border. These characteristic features of osteoclasts in the scales are similar to those in mammals. Most teleosts use the scale as an internal calcium reservoir during the reproductive season. The expression of TRAP and Cath K mRNAs in the scale significantly increased in April, which is a reproductive season, compared with that in October, a non-reproductive season. Thus, both of these molecular markers should be useful for the study of osteoclasts in the teleost scale.
Azuma
Kyoichi
K
Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, Tokyo 169-8050, Japan.
Kobayashi
Masaki
M
Nakamura
Masahisa
M
Suzuki
Nobuo
N
Yashima
Sayaka
S
Iwamuro
Shawichi
S
Ikegame
Mika
M
Yamamoto
Toshio
T
Hattori
Atsuhiko
A
D000135:Acid Phosphatase / Q000096:biosynthesis*; D000595:Amino Acid Sequence; D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D056657:Cathepsin K; D002403:Cathepsins / Q000096:biosynthesis* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005786:Gene Expression Regulation; D006054:Goldfish; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D010010:Osteoclasts / Q000378:metabolism*; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D000071681:Tartrate-Resistant Acid Phosphatase
D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D002403:Cathepsins; D056657:Cathepsin K