Desulfovibrio marinus sp. nov., a moderately halophilic sulfate-reducing bacterium isolated from marine sediments in Tunisia.
Two novel sulfate-reducing bacterial strains, designated E-2(T) and IMP-2, were isolated from geographically distinct locations. Strain E-2(T) was recovered from marine sediments near Sfax (Tunisia), whereas strain IMP-2 originated from oilfield production fluids in the Gulf of Mexico. Cells were Gram-negative, non-sporulated, motile, vibrio-shaped or sigmoid. They were strictly anaerobic, mesophilic and moderately halophilic. Sulfate, sulfite, thiosulfate and elemental sulfur served as electron acceptors, but not nitrate or nitrite. H(2) (with acetate as carbon source), formate, fumarate, lactate, malate, pyruvate, succinate and fructose were used as electron donors in the presence of sulfate as terminal electron acceptor. Lactate was oxidized incompletely to acetate. Fumarate and pyruvate were fermented. Desulfoviridin and c-type cytochromes were present. 16S rRNA gene sequence analysis of the two strains showed that they were phylogenetically similar (99.0 % similarity) and belonged to the genus Desulfovibrio, with Desulfovibrio indonesiensis and Desulfovibrio gabonensis as their closest phylogenetic relatives. The G+C content of the DNA was respectively 60.4 and 62.7 mol% for strains E-2(T) and IMP-2. DNA-DNA hybridization experiments revealed that the novel strains had a high genomic relatedness, suggesting that they belong to the same species. We therefore propose that the two isolates be affiliated to a novel species of the genus Desulfovibrio, Desulfovibrio marinus sp. nov. The type strain is strain E-2(T) (=DSM 18311(T) =JCM 14040(T)).
Ben Dhia Thabet
O
O
Laboratoire d'Ecologie et de Technologie Microbienne, INSAT, 1080 Tunis, Tunisia.|Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, Case 925, 163 Avenue de Luminy, 13288 Marseille cedex 9, France.
Fardeau
M-L
ML
Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, Case 925, 163 Avenue de Luminy, 13288 Marseille cedex 9, France.
Suarez-Nuñez
C
C
Instituto Mexicano del Petróleo, Programa de Biotecnología del Petróleo, 07730 México DF, Mexico.|Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, Case 925, 163 Avenue de Luminy, 13288 Marseille cedex 9, France.
Hamdi
M
M
Laboratoire d'Ecologie et de Technologie Microbienne, INSAT, 1080 Tunis, Tunisia.
Thomas
P
P
Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, Case 925, 163 Avenue de Luminy, 13288 Marseille cedex 9, France.
Ollivier
B
B
Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, Case 925, 163 Avenue de Luminy, 13288 Marseille cedex 9, France.
Alazard
D
D
Instituto Mexicano del Petróleo, Programa de Biotecnología del Petróleo, 07730 México DF, Mexico.|Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, Case 925, 163 Avenue de Luminy, 13288 Marseille cedex 9, France.
D000693:Anaerobiosis / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D045304:Cytochromes c / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003901:Desulfovibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D005285:Fermentation / Q000502:physiology; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D050885:Hydrogensulfite Reductase / Q000032:analysis; D008124:Locomotion / Q000502:physiology; D008800:Mexico; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013431:Sulfates / Q000378:metabolism; D013696:Temperature; D014416:Tunisia; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D045304:Cytochromes c; D050885:Hydrogensulfite Reductase