title: Subtypes of B lymphocytes in patients with autoimmune hemocytopenia.
abstract: OBJECTIVE
  To investigate the quantities of bone marrow CD5+ B lymphocytes in the patients with autoimmune hemocytopenia and the relationship between quantities of CD5+ B lymphocytes and clinical or laboratorial parameters.
  
  
  METHODS
  Quantities of CD5+ B lymphocytes in the bone marrow of 14 patients with autoimmune hemolytic anemia (AIHA) or Evans syndrome, 22 immunorelated pancytopenia (IRP) patients, and 10 normal controls were assayed by flow cytometry. The correlation between their clinical or laboratorial parameters and CD5+ B lymphocytes was analyzed.
  
  
  RESULTS
  The quantity of CD5+ B lymphocytes of AIHA/Evans syndrome (34.64% +/- 19.81%) or IRP patients (35.81% +/- 16.83%) was significantly higher than that of normal controls (12.00% +/- 1.97%, P < 0.05). However, there was no significant difference between AIHA/Evans syndrome and IRP patients (P > 0.05). In all hemocytopenic patients, the quantity of bone marrow CD5+ B lymphocytes showed significantly negative correlation with serum complement C3 level (r = -0.416, P < 0.05). In the patients with AIHA/Evans syndrome, the quantity of bone marrow CD5+ B lymphocytes showed significantly positive correlation with serum indirect bilirubin level (r = 1.00, P < 0.05). In Evans syndrome patients, the quantity of CD5+ B lymphocytes in bone marrow showed significantly positive correlation with platelet-associated immunoglobulin G (r = 0.761, P < 0.05) and platelet-associated immunoglobulin M ( r = 0.925, P < 0.05). The quantity of CD5+ B lymphocytes in bone marrow of all hemocytopenic patients showed significantly negative correlation with treatment response (tau-b = -0.289, P < 0.05) , but had no correlation with colony forming unit-erythroid (r = -0.205, P > 0.05) or colony forming unit-granulocyte-macrophage colonies (r = -0.214, P > 0.05).
  
  
  CONCLUSIONS
  The quantity of bone marrow CD5+ B lymphocytes in the patients with autoimmune hemocytopenia significantly increases and is correlated with disease severity and clinical response, which suggest that CD5+ B lymphocytes might play an important role in the pathogenesis of autoimmune hemocytopenia.
authors.affiliation: Department of Hematology and Oncology, General Hospital of Tianjin Medical University, Tianjin 300052.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Xing
authors.forename: Zong-Hong
authors.initials: ZH
authors.lastname: Shao
authors.forename: Rong
authors.initials: R
authors.lastname: Fu
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Shi
authors.forename: Jie
authors.initials: J
authors.lastname: Bai
authors.forename: Mei-Feng
authors.initials: MF
authors.lastname: Tu
authors.forename: Hua-Quan
authors.initials: HQ
authors.lastname: Wang
authors.forename: Zhen-Zhu
authors.initials: ZZ
authors.lastname: Cui
authors.forename: Hai-Rong
authors.initials: HR
authors.lastname: Jia
authors.forename: Juan
authors.initials: J
authors.lastname: Sun
authors.forename: Chong-Li
authors.initials: CL
authors.lastname: Yang
mesh_terms: D000744:Anemia, Hemolytic, Autoimmune / Q000188:drug therapy / Q000276:immunology*; D001327:Autoimmune Diseases / Q000188:drug therapy / Q000276:immunology*; D001402:B-Lymphocytes / Q000145:classification* / Q000276:immunology*; D016572:Cyclosporine / Q000627:therapeutic use; D004359:Drug Therapy, Combination; D005434:Flow Cytometry; D005938:Glucocorticoids / Q000627:therapeutic use; D006801:Humans
chemical_list: D005938:Glucocorticoids; D016572:Cyclosporine