title: Expression profiles of BDNF splice variants in cultured DRG neurons stimulated with NGF.
abstract: Expression of brain-derived neurotrophic factor (BDNF) mRNA is increased in the dorsal root ganglion (DRG) in response to peripheral inflammation. Nerve growth factor (NGF) from inflammatory tissue is thought to induce expression of BDNF. Recently, it was reported that the BDNF gene has eight non-coding exons that are transcribed independently into several splice variants. Expression of these splice variants in DRG neurons stimulated with NGF has not been studied. We examined changes in expression of BDNF splice variants in a rat model of peripheral inflammation and in cultured DRG neurons exposed to NGF. Total BDNF mRNA was increased by inflammation in vivo and by NGF in vitro. Among all splice variants, exon 1-9 showed the greatest increase in expression in both experiments. Our results indicate that exon 1-9 contributes to changes in total BDNF levels and may play an important role in the acute response of DRG to NGF.
authors.affiliation: Department of Anesthesiology and Resuscitology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama City 700-8558, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Matsuoka
authors.forename: Masataka
authors.initials: M
authors.lastname: Yokoyama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Megumi
authors.initials: M
authors.lastname: Omori
authors.forename: Yoshitaro
authors.initials: Y
authors.lastname: Itano
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Morita
authors.forename: Hiroki
authors.initials: H
authors.lastname: Mori
authors.forename: Tohru
authors.initials: T
authors.lastname: Nakanishi
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics*; D002478:Cells, Cultured; D005091:Exons; D005727:Ganglia, Spinal / Q000378:metabolism*; D005786:Gene Expression Regulation; D007249:Inflammation; D008297:Male; D020932:Nerve Growth Factor / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry; D010146:Pain; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D009841:Oligonucleotides; D020932:Nerve Growth Factor