title: A step-by-step tutorial to use HierFstat to analyse populations hierarchically structured at multiple levels.
abstract: The populations of parasites and infectious agents are most of the time structured in complex hierarchy that lies beyond the classical nested design described by Wright's F-statistics (F(IS), F(ST) and F(IT)). In this note we propose a user-friendly step-by-step notice for using recent software (HierFstat) that computes and test fixation indices for any hierarchical structure. We add some tricks and tips for some special data kind (haploid, single locus), some other procedure (bootstrap over loci) and how to handle crossed factors.
authors.affiliation: Génétique et Evolution des Maladies Infectieuses, Unité Mixte de Recherche 2724, Institute de Recherche pour le Développement, Centre National de la Recherche Scientifique, Centre IRD, BP 64501, 34394 Montpellier Cedex 5, France. demeeus@mpl.ird.fr
authors.forename: Thierry
authors.initials: T
authors.lastname: de Meeûs
authors.forename: Jérôme
authors.initials: J
authors.lastname: Goudet
mesh_terms: D005828:Genetics, Population; D012984:Software