[Mechanism and protective effect of glossy ganoderma decoction on the activities of RNA polymerase in hepatocyte of rabbits with Amanita mushroom poisoning].
OBJECTIVE
To investigate the mechanism of glossy ganoderma decoction in Amanita mushroom poisoning.


METHODS
Twenty male New Zealand white rabbits were randomly divided into 4 groups, including a normal control, a model poison group, and 2 treatment groups (different doses of glossy ganoderma decoction). The activities of hepatocyte RNA polymerase were measured by ultraviolet spectrophotometry and liver function were measured.


RESULTS
The activities of hepatocyte RNA polymerase of the model group significantly decreased, and those of the 2 treatment groups were significantly higher than those of the model group. There was a dose-dependent manner between the 2 treatment groups ( all Ps<0.01), and the differences of liver function test including total bilirubin (TBIL), direct bilirubin (DB), total bile acid (TBA), and alanine aminotransferase (ALT) in the 4 groups were significant (P<0.01).


CONCLUSIONS
Glossy ganoderma decoction may protect the liver from Amanita mushroom poisoning. Its mechanism may be related to the increase of the activities of hepatocyte RNA polymerase.
Xiao
Gui-Lin
GL
Department of Emergency, Xiangya Hospital, Central South University, Changsha 410008, China. xiaoguilinxy@126.com
Yang
Ning
N
Zhang
Chun-Hu
CH
Hu
Sui-Yu
SY
D000410:Alanine Transaminase / Q000378:metabolism; D000545:Amanita; D000818:Animals; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D032902:Ganoderma; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008516:Medicine, Chinese Traditional; D009145:Mushroom Poisoning / Q000188:drug therapy / Q000503:physiopathology*; D011817:Rabbits
D000410:Alanine Transaminase; D012321:DNA-Directed RNA Polymerases