DARPP-32 and NCS-1 expression is not altered in brains of rats treated with typical or atypical antipsychotics.
Dopamine-mediated neurotransmission imbalances are associated with several psychiatry illnesses, such as schizophrenia. Recently it was demonstrated that two proteins involved in dopamine signaling are altered in prefrontal cortex (PFC) of schizophrenic patients. DARPP-32 is a key downstream effector of intracellular signaling pathway and is downregulated in PFC of schizophrenic subjects. NCS-1 is a neuronal calcium sensor that can inhibit dopamine receptor D2 internalization and is upregulated in PFC of schizophrenic subjects. It is well known that dopamine D2 receptor is the main target of antipsychotic. Therefore, our purpose was to study if chronic treatment with typical or atypical antipsychotics induced alterations in DARPP-32 and NCS-1 expression in five brain regions: prefrontal cortex, hippocampus, striatum, cortex and cerebellum. We did not find any changes in DARPP-32 and NCS-1 protein expression in any brain region investigated.
Souza
Bruno R
BR
Laboratório de Neurociências, Departamento de Saúde Mental, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av Alfredo Balena 190, Belo Horizonte, MG 30130-100, Brazil.
Motta
Bernardo S
BS
Rosa
Daniela V F
DV
Torres
Karen C L
KC
Castro
Adalberto A
AA
Comim
Clarissa M
CM
Sampaio
André M
AM
Lima
Fabrício F
FF
Jeromin
Andreas
A
Quevedo
João
J
Romano-Silva
Marco A
MA
D000818:Animals; D014150:Antipsychotic Agents / Q000494:pharmacology*; D015153:Blotting, Western; D001923:Brain Chemistry / Q000187:drug effects*; D003720:Densitometry; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32 / Q000096:biosynthesis*; D008297:Male; D051580:Neuronal Calcium-Sensor Proteins / Q000096:biosynthesis*; D009479:Neuropeptides / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D017448:Receptors, Dopamine D2 / Q000096:biosynthesis; D015854:Up-Regulation / Q000187:drug effects
D014150:Antipsychotic Agents; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides; C497930:Ppp1r1b protein, rat; D017448:Receptors, Dopamine D2; C101464:frequenin calcium sensor proteins