Evidence for the dimerization of human regulator of G-protein signalling 5 (RGS5).
RGS5 is a R4 type RGS that regulates GPCR signalling. Using western blot, we detected RGS5 as a specific 23 kDa protein in cells overexpressing RGS5. A 42 kDa band representing a possible RGS5 dimer was also detected. Given that GPCRs and their associated proteins form complexes involving multiple protein-protein interactions, we investigated the possibility that the 42 kDa band represents an RGS5-RGS5 dimer. RGS5 dimerization was confirmed by the analysis of a GFP tagged RGS5 fusion in yeast and with two-hybrid assays. Analysis of RGS5 in HEK293A cells suggests that the dimer may serve a regulatory function since it is longer lived than the monomer.
Yang
Zhao
Z
Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec.
Gaudio
Sabrina
S
Song
Wei
W
Greenwood
Matthew
M
Jean-Baptiste
Gaël
G
Greenwood
Michael T
MT
D002460:Cell Line; D019281:Dimerization; D006801:Humans; D020710:RGS Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D020710:RGS Proteins; C491349:RGS5 protein, human; D011994:Recombinant Proteins