Cerasicoccus arenae gen. nov., sp. nov., a carotenoid-producing marine representative of the family Puniceicoccaceae within the phylum 'Verrucomicrobia', isolated from marine sand.
A polyphasic taxonomic study was performed on strain YM26-026(T), which was isolated from acid-treated sediment in Kamaishi, Japan. The bacterial cells were pale-pink-pigmented, Gram-negative, obligately aerobic, non-spore-forming, spherical and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate was a member of the phylum 'Verrucomicrobia' and shared approximately 84-87 % sequence similarity with strains of the class Opitutae that have been cultivated to date. Strain YM26-026(T) produced pale-pink pigments of carotenoid. beta-Lactam antibiotic susceptibility tests and amino acid analysis of cell-wall hydrolysates indicated that the novel isolate did not contain muramic acid or diaminopimelic acid in the cell wall, suggesting that the strain lacks peptidoglycan. The G+C content of the DNA of strain YM26-026(T) was 54.0 mol%. Menaquinone-7 was the major quinone and C(14 : 0) and C(18 : 1)omega9c were the major fatty acids. On the basis of polyphasic taxonomic studies, it was concluded that strain YM26-026(T) represents a new genus of the family Puniceicoccaceae within the phylum 'Verrucomicrobia', for which the name Cerasicoccus arenae gen. nov., sp. nov. is proposed. The type strain is YM26-026(T) (=MBIC08280(T)=KCTC 12870(T)).
Yoon
Jaewoo
J
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan.
Matsuo
Yoshihide
Y
Marine Biotechnology Institute Co. Ltd, 3-75-1, Heita, Kamaishi, Iwate 026-0001, Japan.
Matsuda
Satoru
S
Marine Biotechnology Institute Co. Ltd, 3-75-1, Heita, Kamaishi, Iwate 026-0001, Japan.
Adachi
Kyoko
K
Marine Biotechnology Institute Co. Ltd, 3-75-1, Heita, Kamaishi, Iwate 026-0001, Japan.
Kasai
Hiroaki
H
Marine Biotechnology Institute Co. Ltd, 3-75-1, Heita, Kamaishi, Iwate 026-0001, Japan.
Yokota
Akira
A
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan.
D000332:Aerobiosis / Q000502:physiology; D000596:Amino Acids / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D050260:Carbohydrate Metabolism; D002338:Carotenoids / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D007564:Japan; D008124:Locomotion / Q000502:physiology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009112:Muramic Acids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012822:Silicon Dioxide; D013171:Spores, Bacterial / Q000166:cytology; D047090:beta-Lactams / Q000494:pharmacology
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009112:Muramic Acids; D010860:Pigments, Biological; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D047090:beta-Lactams; D002338:Carotenoids; D003960:Diaminopimelic Acid; D012822:Silicon Dioxide