Functional study of Capsicum annuum fatty acid desaturase 1 cDNA clone induced by Tobacco mosaic virus via microarray and virus-induced gene silencing.
A series of microarray analyses employing the expressed sequence tags (ESTs) of hot pepper was conducted in an effort to elucidate the molecular mechanisms inherent to hypersensitive response (HR) by viral or bacterial pathogens. There were 2535 ESTs exhibiting differential expression (over 2-fold changes) among about 5000 ESTs during viral or bacterial response. Further, via virus-induced gene silencing (VIGS) and TMV-infection studies, we were able to isolate several ESTs, which may be relevant to defense response against TMV. Of these ESTs, Capsicum annuum fatty acid desaturase 1 (CaFAD1) showed the distinct phenotype against TMV infection and thus was subjected to further study. CaFAD1-silenced plants showed weaker resistance against TMV-P0 infection compared to TRV2 control plants. Also the suppression of FAD1 expression caused blocking of cell death induced by Bcl2-associated X (Bax) protein in tobacco plants. Therefore, this report presents that both microarray and VIGS approaches are feasible in hot pepper plants and the TMV-induced CaFAD1 plays a role in HR response.
Kim
Ki-Jeong
KJ
School of Life Sciences and Biotechnology/Plant Signaling Network Research Center, Korea University, 1,5-ga, Anam-dong, Sungbuk-gu, Seoul 136-701, Republic of Korea.
Lim
Jee Hyuck
JH
Lee
Sanghyeob
S
Kim
Young Jin
YJ
Choi
Soo Bok
SB
Lee
Min Kyung
MK
Choi
Doil
D
Paek
Kyung-Hee
KH
D002212:Capsicum / Q000201:enzymology* / Q000821:virology*; D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D044943:Fatty Acid Desaturases / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010641:Phenotype; D018515:Plant Leaves / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012231:Rhizobium / Q000378:metabolism; D015398:Signal Transduction; D014026:Nicotiana / Q000378:metabolism; D014027:Tobacco Mosaic Virus / Q000378:metabolism*
D044943:Fatty Acid Desaturases