Allelic polymorphism of MSP2 gene in severe P. falciparum malaria in an area of low and seasonal transmission.
The severe malaria (SM) and uncomplicated malaria (UM) infections are expected to have different genetic makeup. In this study, blood samples were obtained from 325 donors with SM and UM and malaria-free donors (including asymptomatic submicroscopic malaria--ASUM), from Eastern Sudan. The SM group included patients with cerebral malaria (CM), severe malarial anemia (SMA), and other complications. The MSP2 locus was exploited for parasite genotyping. We found that the genetic diversity of the parasite population was marked (51 genotypes). The overall multiplicity of infection (MOI) was 1.5, and it was comparable between SM and UM. However, the MOI in ASUM (1.0) and fatal CM (1.14) was comparable and significantly lower than in UM (1.53), SMA (1.52), and nonfatal CM (1.7). The ratio of the IC1 to FC27 allele families was comparable between SM and UM, and the distribution of the allele sizes was correlated (correlation coefficient = 0.59 and 0.718; P < 0.001). It is interesting to note that the FC27 genotype was overrepresented in ASUM (68.2%) and was not recognized in fatal CM, while in mixed-clone infections, the clearance of IC1 after quinine treatment was faster than FC27 clearance. Finally, the composition of the multiclone infections (IC1 and FC27) was suggesting a stronger cross-immunity within rather than between MSP2 gene families.
A-Elbasit
Ishraga E
IE
Malaria Research Centre (MalRC), Department of Biochemistry, University of Khartoum, P.O. Box 102, Khartoum, Sudan.
ElGhazali
Gehad
G
A-Elgadir
Thoraya M E
TM
Hamad
Amel A
AA
Babiker
Hamza A
HA
Elbashir
Mustafa I
MI
Giha
Hayder A
HA
D000293:Adolescent; D000483:Alleles; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D002648:Child; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology* / Q000635:transmission*; D008297:Male; D010963:Plasmodium falciparum / Q000235:genetics*; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics*; D012621:Seasons
D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium