title: Activity-directed-fractionation and isolation of four antibacterial compounds from Abrus precatorius L., roots.
abstract: Root extracts of the plant Abrus precatorius L., was tested for antibacterial activity. Various solvent fractions exhibited inhibitory activity against 13 gram-positive and gram-negative bacteria. Root extracts were analyzed by thin layer chromatography (TLC). The antibacterial activity was localized to specific chromatophores in the chloroform fraction through a bioautography assay. It was found localized to four chromatophores out of seven. The chromatophores were isolated from the TLC plates and rechecked for activity against Slaphylococcus aureus A, using a disc diffusion assay. Among the four active principles isolated, AP 3 (Rf 0.87) exhibited maximum activity i.e. 56% inhibition of growth of S. aureus A, in disc diffusion assay compared to the standard antibiotic Ampicillin. Results of this study suggest that chloroform extractable phytochemicals in A. precatorius L. may yield promising molecules with antibiotic activity.
authors.affiliation: School of Life Sciences, SRTM University, Nanded (MS), India.
authors.forename: G B
authors.initials: GB
authors.lastname: Zore
authors.forename: V
authors.initials: V
authors.lastname: Awad
authors.forename: A D
authors.initials: AD
authors.lastname: Thakre
authors.forename: U K
authors.initials: UK
authors.lastname: Halde
authors.forename: N S
authors.initials: NS
authors.lastname: Meshram
authors.forename: B S
authors.initials: BS
authors.lastname: Surwase
authors.forename: S Mohan
authors.initials: SM
authors.lastname: Karuppayil
mesh_terms: D029912:Abrus / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D002855:Chromatography, Thin Layer; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts