IMPG1 gene variation in rhesus macular drusen.
Drusen is a hallmark of human age-related maculopathy. Rhesus macaques (Macaca mulatta) represent a natural model of age-related maculopathy with drusen. We have already mapped the macular drusen susceptibility locus in rhesus macaques to the homolog of human chromosome 6q14-15 and shown that a particular IMPG1 gene SNP haplotype was apparently associated with drusen formation in the rhesus macaques maintained by the Caribbean Primate Research Center (CPRC), Puerto Rico, USA. The aim of the present study was to verify this finding in the macaques kept at the German Primate Research Center (DPZ), Germany. The study group comprised 64 animals (34 affected, 30 unaffected). These monkeys were genotyped for all known variations in the IMPG1 gene and haplotype analysis was performed. A total absence of the previously identified risk haplotype of the IMPG1 gene, and a much lower drusen prevalence in comparison to the CPRC group, was observed in the DPZ samples. This prompted a re-analysis of the original disease association in the CPRC, which revealed that the implied risk haplotype was in fact a sequencing artifact. Taken together, the data highlight that additional factors, other than IMPG1 variation, must play a role in drusen pathogenesis in rhesus macaques.
Singh
Krishna K
KK
Institut für Humangenetik, Medizinische Hochschule Hannover, Hannover, Germany.
Dawson
William W
WW
Krawczak
Michael
M
Schmidtke
Jörg
J
D000818:Animals; D016022:Case-Control Studies; D004195:Disease Models, Animal; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D008253:Macaca mulatta; D008268:Macular Degeneration / Q000235:genetics / Q000662:veterinary; D008992:Monkey Diseases / Q000235:genetics*; D015593:Retinal Drusen / Q000235:genetics / Q000662:veterinary*
D005819:Genetic Markers