Chicory increases acetate turnover, but not propionate and butyrate peripheral turnovers in rats.
Chicory roots are rich in inulin that is degraded into SCFA in the caecum and colon. Whole-body SCFA metabolism was investigated in rats during food deprivation and postprandial states. After 22 h of food deprivation, sixteen rats received an IV injection of radioactive 14C-labelled SCFA. The volume of distribution and the fractional clearance rate of SCFA were 0.25-0.27 litres/kg and 5.4-5.9 %/min, respectively. The half-life in the first extracellular rapidly decaying compartment was between 0.9 and 1.4 min. After 22 h of food deprivation, another seventeen rats received a primed continuous IV infusion of 13C-labelled SCFA for 2 h. Isotope enrichment (13C) of SCFA was determined in peripheral arterial blood by MS. Peripheral acetate, propionate and butyrate turnover rates were 29, 4 and 0.3 micromol/kg per min respectively. Following 4 weeks of treatment with chicory root or control diets, eighteen fed rats received a primed continuous IV infusion of 13C-labelled SCFA for 2 h. Intestinal degradation of dietary chicory lowered caecal pH, enhanced caecal and colonic weights, caecal SCFA concentrations and breath H2. The diet with chicory supplementation enhanced peripheral acetate turnover by 25 % (P = 0.017) concomitant with an increase in plasma acetate concentration. There were no changes in propionate or butyrate turnovers. In conclusion, by setting up a multi-tracer approach to simultaneously assess the turnovers of acetate, propionate and butyrate it was demonstrated that a chronic chicory-rich diet significantly increases peripheral acetate turnover but not that of propionate or butyrate in rats.
Pouteau
Etienne
E
Nestlé Research Centre, Nutrition and Health Department, Vers-Chez-Les-Blanc, PO Box 44, 1000 Lausanne 26, Switzerland. etienne.pouteau@rdls.nestle.com
Rochat
Florence
F
Jann
Alfred
A
Meirim
Isabelle
I
Sanchez-Garcia
Jose-Luis
JL
Ornstein
Kurt
K
German
Bruce
B
Ballèvre
Olivier
O
D000085:Acetates / Q000097:blood*; D000818:Animals; D002087:Butyrates / Q000097:blood; D002250:Carbon Radioisotopes; D002432:Cecum / Q000378:metabolism; D018651:Cichorium intybus; D003106:Colon / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000096:biosynthesis / Q000097:blood*; D005260:Female; D005508:Food Deprivation / Q000502:physiology; D006207:Half-Life; D008297:Male; D011169:Portal Vein / Q000378:metabolism; D019518:Postprandial Period / Q000502:physiology; D011422:Propionates / Q000097:blood; D051381:Rats
D000085:Acetates; D002087:Butyrates; D002250:Carbon Radioisotopes; D005232:Fatty Acids, Volatile; D011422:Propionates