Effect of a small, acid-soluble spore protein from Clostridium perfringens on the resistance properties of Bacillus subtilis spores.
Alpha/beta-type small, acid-soluble spore proteins (SASP) are essential for the resistance of DNA in spores of Bacillus species to damage. An alpha/beta-type SASP, Ssp2, from Clostridium perfringens was expressed at significant levels in B. subtilis spores lacking one or both major alpha/beta-type SASP (alpha- and alpha- beta- strains, respectively). Ssp2 restored some of the resistance of alpha- beta- spores to UV and nitrous acid and of alpha- spores to dry heat. Ssp2 also restored much of the resistance of alpha- spores to nitrous acid and restored full resistance of alpha- spores to UV and moist heat. These results further indicate the interchangeability of alpha/beta-type SASP in DNA protection in spores.
Leyva-Illades
Juan Francisco
JF
Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030-3305, USA.
Setlow
Barbara
B
Sarker
Mahfuzur R
MR
Setlow
Peter
P
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D003016:Clostridium perfringens / Q000235:genetics / Q000502:physiology*; D017931:DNA Primers; D007700:Kinetics; D008969:Molecular Sequence Data; D013171:Spores, Bacterial / Q000187:drug effects / Q000502:physiology*
D001426:Bacterial Proteins; D017931:DNA Primers