title: Effect of plasma from cyclic versus nutritionally induced anovulatory beef heifers on proliferation of granulosa cells in vitro.
abstract: The effect of plasma from cyclic versus nutritionally induced anovulatory beef heifers was evaluated on proliferation of bovine granulosa cells in vitro. Granulosa cells were obtained from small (1-5mm) follicles of cattle and cultured for 4 days. During the last 2 days of culture, cells were exposed to medium containing 0, 1 or 10% plasma from cyclic or anovulatory heifers in the presence or absence of IGF-I (100ng/ml). Cell numbers were determined. Regardless of source, increasing percentage of plasma to culture medium increased cell numbers. However, the plasma-induced increase was greater in granulosa cells exposed to cyclic heifer plasma versus anovulatory heifer plasma. In addition, concomitant treatment with IGF-I dramatically improved cell proliferation induced by anovulatory heifer plasma. These results indicate that plasma from cyclic heifers contain factors that are a greater stimulus to granulosa cell proliferation than plasma from anovulatory heifers. Systemic factors such as IGF-I may play a role in directly regulating granulosa cell proliferation in cattle.
authors.affiliation: Department of Animal Science, Oklahoma State University, Stillwater, OK 74078, United States. leon.spicer@okstate.edu
authors.forename: L J
authors.initials: LJ
authors.lastname: Spicer
authors.forename: I
authors.initials: I
authors.lastname: Bossis
authors.forename: R P
authors.initials: RP
authors.lastname: Wettemann
mesh_terms: D000818:Animals; D000858:Anovulation / Q000097:blood* / Q000209:etiology; D002417:Cattle / Q000097:blood*; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D004032:Diet; D030762:Estrous Cycle / Q000097:blood*; D005260:Female; D006107:Granulosa Cells / Q000166:cytology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I