Expression Profile of Wnt Molecules in Leukemic Cells from Iranian Patients with Acute Myeloblastic Leukemia
Wnt molecules play a key role in growth, proliferation and development of some embryonic and adult organs as well as hematopoietic stem cells. Wnt signaling pathways are aberrantly activated in many tumor types, including solid tumors and hematologic malignancies.



To investigate the expression profile of a large number of Wnt genes in leukemic cells from Iranian patients with acute myeloblastic leukemia.



RT-PCR method was used to determine the Wnt genes expression in bone marrow (BM) and/or peripheral blood (PB) samples from 16 patients with AML and PB samples of 36 normal subjects.



Among 14 Wnt molecules included in this study, Wnt-7A and Wnt-10A were significantly down-regulated (p = 0.002 and p < 0.0001, respectively) and Wnt-3 was significantly over-expressed (p < 0.02) in AML patients compared to normal subjects. No significant association was found between Wnt expression and FAB classification of the patients.



Our results demonstrated for the first time aberrant expression of Wnt-7A, Wnt-10A and Wnt-3 genes in Iranian AML patients. This may be of relevance to the tumorigenesis process in this malignancy.
Memarian
Ali
A
Department of Immunology, School of Public Health, Medical Sciences, University of Tehran, Tehran, Iran.
Jeddi Tehrani
Mahmood
M
Vossough
Parvaneh
P
Sharifian
Ramazan Ali
RA
Rabbani
Hodjatallah
H
Shokri
Fazel
F
D000293:Adolescent; D000328:Adult; D001854:Bone Marrow Cells / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007223:Infant; D007492:Iran; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism*; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051153:Wnt Proteins / Q000097:blood / Q000235:genetics* / Q000378:metabolism
D051153:Wnt Proteins