title: Characterization of bixin by LC-MS and LC-NMR.
abstract: An overview upon modern analytical techniques for the isolation, separation, and structural identification of the essential bioactive carotenoid bixin is given. Isolation from biological matrices is performed by matrix solid phase dispersion (MSPD). The extract is separated with shape-selective C(30 )columns. Structural assignment of the separated compounds is done by online LC-MS and capillary HPLC-NMR.
authors.affiliation: Institute of Organic Chemistry, University of Tuebingen, Tuebingen, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Rehbein
authors.forename: Benjamin
authors.initials: B
authors.lastname: Dietrich
authors.forename: Marc David
authors.initials: MD
authors.lastname: Grynbaum
authors.forename: Petra
authors.initials: P
authors.lastname: Hentschel
authors.forename: Karsten
authors.initials: K
authors.lastname: Holtin
authors.forename: Maximilian
authors.initials: M
authors.lastname: Kuehnle
authors.forename: Paul
authors.initials: P
authors.lastname: Schuler
authors.forename: Marc
authors.initials: M
authors.lastname: Bayer
authors.forename: Klaus
authors.initials: K
authors.lastname: Albert
mesh_terms: D000096:Acetone / Q000032:analysis; D002338:Carotenoids / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods*; D004867:Equipment Design; D005609:Free Radicals; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D008956:Models, Chemical; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D020033:Protein Isoforms
chemical_list: D005609:Free Radicals; D010936:Plant Extracts; D020033:Protein Isoforms; D000096:Acetone; D002338:Carotenoids; C004588:bixin