title: Tau is endogenously nitrated in mouse brain: identification of a tyrosine residue modified in vivo by NO.
abstract: Nitration of tau protein is normally linked to neurodegeneration but, until now, no comprehensive information is available regarding tau nitration in healthy subjects. It has been previously reported that in differentiated PC12 cells, tau co-immunoprecipitated with alpha-tubulin is nitrated at tyrosine residues and that this post-translation modification doesn't impair the association of tau with the cytoskeleton. The present paper is focused on the identification of tyrosine residues endogenously modified in tau from PC12 cells and reports for the first time that tau is also nitrated in vivo in normal mouse brain and that one tyrosine is endogenously modified.
authors.affiliation: DIPAV - Section of Biochemistry, University of Milano, Via Celoria 10, Milano 20100, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Nonnis
authors.forename: Graziella
authors.initials: G
authors.lastname: Cappelletti
authors.forename: Francesca
authors.initials: F
authors.lastname: Taverna
authors.forename: Cristina
authors.initials: C
authors.lastname: Ronchi
authors.forename: Severino
authors.initials: S
authors.lastname: Ronchi
authors.forename: Armando
authors.initials: A
authors.lastname: Negri
authors.forename: Eleonora
authors.initials: E
authors.lastname: Grassi
authors.forename: Gabriella
authors.initials: G
authors.lastname: Tedeschi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001923:Brain Chemistry / Q000187:drug effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D006863:Hydrogen-Ion Concentration; D047468:Immunoprecipitation; D007536:Isomerism; D051379:Mice; D009566:Nitrates / Q000378:metabolism*; D009569:Nitric Oxide / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014443:Tyrosine / Q000378:metabolism*; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D009566:Nitrates; D016875:tau Proteins; D009569:Nitric Oxide; D014443:Tyrosine