Biodegradable microspheres containing poly(epsilon-caprolactone)-Pluronic block copolymers for temperature-responsive release of proteins.
Temperature-responsive microspheres were fabricated for the purpose of releasing protein in responsive to surrounding temperature changes. Temperature-responsive polymer, Pluronic was synthesized into block copolymers of poly(epsilon-caprolactone)-Pluronic with two different chain lengths of poly(epsilon-caprolactone). Microspheres loaded with proteins were prepared by a W/O/W emulsion method. The surface morphology was examined by scanning electron microscopy, showing that microspheres with diblock copolymers had porous structures due to hydrophilicity of Pluronic blocks. After incubating the microsphere at 37 degrees C for 7 days, temperature-responsive protein release was monitored with alternating temperature changes between 20 and 37 degrees C. The protein release was attenuated when the microsphere was incubated at 20 degrees C but the release rate was recovered at 37 degrees C, confirming variable release rate according to the temperature changes. The variable release rate of protein was dependent on the length of poly(epsilon-caprolactone) blocks attached to Pluronic.
Lee
Jung Im
JI
Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University, Hyoja2-dong, Chuncheon 200-701, Republic of Korea.
Yoo
Hyuk Sang
HS
D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D020442:Poloxamer / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D011506:Proteins / Q000008:administration & dosage*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage; D013696:Temperature
D011091:Polyesters; D011506:Proteins; D020442:Poloxamer; C016240:polycaprolactone; D012710:Serum Albumin, Bovine