title: MicroRNAs and regeneration: Let-7 members as potential regulators of dedifferentiation in lens and inner ear hair cell regeneration of the adult newt.
abstract: MicroRNAs are known to regulate the expression of many mRNAs by binding to complementary target sequences at the 3'UTRs. Because of such properties, miRNAs may regulate tissue-specific mRNAs as a cell undergoes transdifferentiation during regeneration. We have tested this hypothesis during lens and hair cell regeneration in newts using microarray analysis. We found that distinct sets of miRNAs are associated with lens and hair cell regeneration. Members of the let-7 family are expressed in both events and they are regulated in a similar fashion. All the let-7 members are down regulated during the initiation of regeneration, which is characterized by dedifferentiation of terminally differentiated cells. This is the first report to correlate expression of miRNAs as novel regulators of vertebrate regeneration, alluding to a novel mechanism whereby transdifferentiation occurs.
authors.affiliation: Department of Biology and Center for Tissue Regeneration and Engineering, University of Dayton, Dayton, OH 45469-2320, USA. panagiotis.tsonis@notes.udayton.edu
authors.forename: Panagiotis A
authors.initials: PA
authors.lastname: Tsonis
authors.forename: Mindy K
authors.initials: MK
authors.lastname: Call
authors.forename: Matthew W
authors.initials: MW
authors.lastname: Grogg
authors.forename: Maureen A
authors.initials: MA
authors.lastname: Sartor
authors.forename: Ruth R
authors.initials: RR
authors.lastname: Taylor
authors.forename: Andrew
authors.initials: A
authors.lastname: Forge
authors.forename: Robert
authors.initials: R
authors.lastname: Fyffe
authors.forename: Robert
authors.initials: R
authors.lastname: Goldenberg
authors.forename: Richard
authors.initials: R
authors.lastname: Cowper-Sal-lari
authors.forename: Craig R
authors.initials: CR
authors.lastname: Tomlinson
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology; D006199:Hair Cells, Auditory, Inner / Q000166:cytology* / Q000378:metabolism*; D007908:Lens, Crystalline / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics; D009024:Morphogenesis / Q000502:physiology; D020543:Proteome / Q000378:metabolism*; D012038:Regeneration / Q000502:physiology*; D012455:Salamandridae / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D020543:Proteome