title: Chitotriosidase deficiency is not associated with human hookworm infection in a Papua New Guinean population.
abstract: Human chitotriosidase (CHIT1) is a chitinolytic enzyme with suggested anti-fungal properties. Previous studies have suggested that chitotriosidase may also protect individuals against filarial nematode infections and malaria. A mutant allele, which renders chitotriosidase unstable and enzymatically inactive, is found at a frequency of >20% in Caucasians and other populations. This allele is found at much lower frequency in parts of West Africa where malarial and intestinal helminth infections are endemic. Here, we investigate whether there is a significant association between chitotriosidase genotype and the intensity of hookworm infection in 693 individuals from five villages in Papua New Guinea. Individuals were genotyped for chitotriosidase using a PCR-based assay. There was no association between CHIT1 genotype and the intensity of hookworm infection as determined by faecal egg counts. The frequency of the mutant allele was 0.251, very similar to that found in non-endemic countries. The extent of geographical variation in allele frequencies across worldwide populations was not high (F(st)=0.11), and does not provide evidence for directional selection at this locus between different geographical areas. We conclude that the CHIT1 genotype does not play a crucial role in protection against hookworm infection. This does not correlate with a previous study that linked the mutant CHIT1 genotype to filariasis susceptibility. The possible reasons for this discrepancy are discussed.
authors.affiliation: Immune Modulation Research Group, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, United Kingdom.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Hall
authors.forename: Rupert J
authors.initials: RJ
authors.lastname: Quinnell
authors.forename: Andrew
authors.initials: A
authors.lastname: Raiko
authors.forename: Moses
authors.initials: M
authors.lastname: Lagog
authors.forename: Peter
authors.initials: P
authors.lastname: Siba
authors.forename: Shaun
authors.initials: S
authors.lastname: Morroll
authors.forename: Franco H
authors.initials: FH
authors.lastname: Falcone
mesh_terms: D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D005787:Gene Frequency; D005838:Genotype; D006596:Hexosaminidases / Q000235:genetics*; D006725:Hookworm Infections / Q000201:enzymology / Q000453:epidemiology*; D006801:Humans; D010219:Papua New Guinea / Q000453:epidemiology; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D006596:Hexosaminidases; C086400:chitotriosidase