title: Simultaneous analysis of 260 pesticide residues in agricultural products by gas chromatography/triple quadrupole mass spectrometry.
abstract: A method for simultaneous analysis of about 260 pesticides by gas chromatography coupled to tandem mass spectrometry (GC/MS/MS) with a triple quadrupole analyzer (QqQ) has been studied. The pesticides were extracted with acetonitrile and cleaned up by a bilayer cartridge. A single injection method was developed for the monitoring of all of the targeted pesticides. Two MS/MS transitions were selected for each analyte using the intensity ratio obtained from them as a confirmatory parameter. By using matrix-matched standards, 260 pesticides could be determined in most matrixes with recoveries of 70-120% and a standard deviation of < or = 20 at 2 different fortification levels of 0.02 and 0.1 microg/g. The developed method was applied to the monitoring of 173 agricultural product samples from the local market. The sensitivities of this method were lower than with most of the selective GC detectors, such as flame photometric or single MS. The selectivity of QqQ gives a very clean chromatogram, making compound identification and confirmation easy. The quick and reliable monitoring was achieved by combination with rapid extraction and cleanup.
authors.affiliation: Osaka Prefectural Institute of Public Health, Nakamichi 1-3-69, Higashinari-ku, Osaka 537-0025, Japan. okihasi@iph.pref.osaka.jp
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okihashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takatori
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kitagawa
authors.forename: Yukio
authors.initials: Y
authors.lastname: Tanaka
mesh_terms: D002138:Calibration; D002498:Centrifugation; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002849:Chromatography, Gas / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination; D005638:Fruit / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D012275:Oryza / Q000378:metabolism; D010573:Pesticide Residues / Q000032:analysis*; D010575:Pesticides / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014675:Vegetables / Q000378:metabolism
chemical_list: D010573:Pesticide Residues; D010575:Pesticides