Construction of p16Slux, a novel vector for improved bioluminescent labeling of gram-negative bacteria.
A novel vector has been constructed for the constitutive luminescent tagging of gram-negative bacteria by site-specific integration into the 16S locus of the bacterial chromosome. A number of gram-negative pathogens were successfully tagged using this vector, and the system was validated during murine infections of living animals.
Riedel
Christian U
CU
Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland.
Casey
Pat G
PG
Mulcahy
Heidi
H
O'Gara
Fergal
F
Gahan
Cormac G M
CG
Hill
Colin
C
D000818:Animals; D002876:Chromosomes, Bacterial; D005822:Genetic Vectors; D006090:Gram-Negative Bacteria / Q000235:genetics* / Q000302:isolation & purification; D008164:Luminescent Proteins / Q000378:metabolism*; D051379:Mice; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D011994:Recombinant Proteins
D008164:Luminescent Proteins; D012336:RNA, Ribosomal, 16S; D011994:Recombinant Proteins