Determination of aromatic compounds by high-performance liquid chromatography with on-line photoreactor and peroxyoxalate chemiluminescence detection.
This paper describes a novel high-performance liquid chromatographic (HPLC) method for the determination of aromatic compounds with peroxyoxalate chemiluminescence (PO-CL ) detection following on-line UV irradiation. Aromatic compounds were UV irradiated (254 nm, 15 W) to generate hydrogen peroxide, which was determined via PO-CL detection using a mixture of bis(2,4,6-trichlorophenyl)oxalate (aryloxalate) and 2,4,6,8-tetrathiomorpholinopyrimido[5,4-d]pyrimidine (fluorophore) as a post-column CL reagent. Generation of hydrogen peroxide from aromatic compounds was confirmed using a flow injection analysis (FIA) system incorporating an enzyme column reactor immobilized with catalase. The conditions for UV irradiation were optimized using benzene and monosubstituted benzenes (phenol, benzaldehyde, nitrobenzene and N,N-dimethylaniline) by an HPLC system to evaluate the analytical performance of the proposed system. The detection limits for benzene and monosubstituted benzenes were in the range 2.1-124 pmol/injection at signal:noise (S:N) ratio = 3. Monocyclic and polycyclic hydrocarbons were also employed to investigate their CL properties. The possibility of PO-CL detection for a wide variety of aromatic compounds was shown for the first time.
Ihara
Ayuko
A
Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, Japan.
Kishikawa
Naoya
N
Wada
Mitsuhiro
M
Ohba
Yoshihito
Y
Nakashima
Kenichiro
K
Kuroda
Naotaka
N
D002374:Catalase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D006841:Hydrocarbons, Aromatic / Q000032:analysis* / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D010070:Oxalates / Q000737:chemistry*; D010777:Photochemistry / Q000295:instrumentation / Q000379:methods*; D011743:Pyrimidines / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013997:Time Factors
D006841:Hydrocarbons, Aromatic; D010070:Oxalates; D011743:Pyrimidines; C044622:peroxyoxalate; D006861:Hydrogen Peroxide; D002374:Catalase