title: Evidence for the dimerization of human regulator of G-protein signalling 5 (RGS5).
abstract: RGS5 is a R4 type RGS that regulates GPCR signalling. Using western blot, we detected RGS5 as a specific 23 kDa protein in cells overexpressing RGS5. A 42 kDa band representing a possible RGS5 dimer was also detected. Given that GPCRs and their associated proteins form complexes involving multiple protein-protein interactions, we investigated the possibility that the 42 kDa band represents an RGS5-RGS5 dimer. RGS5 dimerization was confirmed by the analysis of a GFP tagged RGS5 fusion in yeast and with two-hybrid assays. Analysis of RGS5 in HEK293A cells suggests that the dimer may serve a regulatory function since it is longer lived than the monomer.
authors.affiliation: Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yang
authors.forename: Sabrina
authors.initials: S
authors.lastname: Gaudio
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Matthew
authors.initials: M
authors.lastname: Greenwood
authors.forename: Gaël
authors.initials: G
authors.lastname: Jean-Baptiste
authors.forename: Michael T
authors.initials: MT
authors.lastname: Greenwood
mesh_terms: D002460:Cell Line; D019281:Dimerization; D006801:Humans; D020710:RGS Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D020710:RGS Proteins; C491349:RGS5 protein, human; D011994:Recombinant Proteins