title: Measurement of the total antioxidant potential in chronic obstructive pulmonary diseases with a novel automated method.
abstract: OBJECTIVE
  To determine the oxidative and antioxidative status of plasma of patients with chronic obstructive pulmonary disease (COPD) and to compare these values with healthy smokers and healthy non-smokers control subjects using a more recently developed automated measurement method.
  
  
  METHODS
  This study involved 40 COPD patients, 25 healthy smokers, and 25 healthy non-smokers who attended the Chest Diseases Outpatient Clinic in Harran University Research Hospital, Turkey during the period between March 2006 and June 2006. We calculated the total antioxidant potential (TAOP) to determine the antioxidative status of plasma, and we measured the total peroxide levels to determine the oxidative status of plasma.
  
  
  RESULTS
  The TAOP of plasma was significantly lower in patients with COPD than in healthy smokers and healthy non-smokers (p<0.001). In contrast, the mean total peroxide level of plasma was significantly higher in COPD patients than in healthy smokers and healthy non-smokers (p<0.001).
  
  
  CONCLUSIONS
  We found a decreased in TAOP COPD patients using a simple, rapid and reliably automated colorimetric assay, which may suitable for use in routine clinical biochemistry laboratory, and considerably facilitates the assessment of this useful clinical parameter. We suggest that this novel method may be used as a routine test to evaluate and follow-up the levels of oxidative stress in COPD.
authors.affiliation: Department of Chest Disease, Harran University, Faculty of Medicine, Turkey. drerkanceylan@yahoo.com
authors.forename: Erkan
authors.initials: E
authors.lastname: Ceylan
authors.forename: Mehmet
authors.initials: M
authors.lastname: Gencer
authors.forename: Elmas
authors.initials: E
authors.lastname: Uzer
authors.forename: Hakim
authors.initials: H
authors.lastname: Celik
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003124:Colorimetry / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D010545:Peroxides / Q000097:blood*; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood*; D012907:Smoking / Q000097:blood*
chemical_list: D010545:Peroxides