Separation and evaluation of free radical-scavenging activity of phenol components of green, brown, and black leaves of Bergenia crassifolia by using HPTLC-DPPH* method.
A new procedure has been developed to separate and quantify the free radical-scavenging activity of individual compounds from green, brown, and black leaves of Bergenia crassifolia based on the combination of high performance TLC (HPTLC) with a diode array detector (DAD) and postchromatographic 1,1-diphenyl-2-picrylhydrazyl radical (DPPH(*)) derivatization. Free gallic and ellagic acids, arbutin, hydroquinone, and bergenin in the B. crassifolia leaves' extracts were separated by HPTLC and identified. All compounds of the extract excluding bergenin were capable of scavenging DPPH * radicals. From the estimated ID(50) values, it can be seen that the increasing order of activity was gallic acid > arbutin > ellagic acid > hydroquinone > ascorbic acid. The antiradical activity of leaves of B. crassifolia is probably associated to the presence of phenol.
Pozharitskaya
Olga N
ON
Saint-Petersburg Institute of Pharmacy, St. Petersburg, Russia.
Ivanova
Svetlana A
SA
Shikov
Alexander N
AN
Makarov
Valery G
VG
Galambosi
Bertalan
B
D001104:Arbutin / Q000032:analysis; D001205:Ascorbic Acid / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods; D003720:Densitometry / Q000379:methods; D004610:Ellagic Acid / Q000032:analysis; D005609:Free Radicals; D005707:Gallic Acid / Q000032:analysis; D008956:Models, Chemical; D019800:Phenol / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis; D018515:Plant Leaves / Q000378:metabolism; D015203:Reproducibility of Results
D005609:Free Radicals; D010936:Plant Extracts; D004610:Ellagic Acid; D019800:Phenol; D005707:Gallic Acid; D001104:Arbutin; D001205:Ascorbic Acid