Upregulation of myocardial Annexin A5 in hypertensive heart disease: association with systolic dysfunction.
OBJECTIVE
To investigate whether Annexin A5 (AnxA5) is related to hypertensive heart disease (HHD) and whether this relation is dependent of apoptosis.


RESULTS
Hypertensives without cardiac abnormalities (stage A), with left ventricular hypertrophy (LVH) (stage B), and with LVH and clinical manifestations of chronic HF (stage C), were studied. AnxA5 was quantified in endomyocardial biopsies by real time RT-PCR, Western blot, and immunohistochemistry, and apoptosis by DNA fragmentation, caspase-3 activation, and PARP and Bax/Bcl-2 ratios. Plasma AnxA5 was measured by ELISA in samples from the coronary sinus and the antecubital vein. Although AnxA5 mRNA did not change, myocardial and plasma AnxA5 were increased in hypertensives stages B and C compared with normotensives and hypertensives stage A. Myocardial AnxA5 was inversely correlated with parameters assessing systolic function in all hypertensives, this association being independent of apoptosis. Myocardial AnxA5 was directly correlated with plasma AnxA5. Plasma AnxA5 was inversely correlated with systolic function in all hypertensives.


CONCLUSIONS
This cross-sectional study shows that myocardial AnxA5 upregulation is associated with HHD and impairment of systolic function in hypertensive patients, this association being independent of apoptosis. Plasma AnxA5 can be a marker of myocardial AnxA5 upregulation and systolic dysfunction in patients with HHD.
Ravassa
Susana
S
Division of Cardiovascular Sciences, Centre for Applied Medical Research, University of Navarra, Pamplona, Spain.
González
Arantxa
A
López
Begoña
B
Beaumont
Javier
J
Querejeta
Ramón
R
Larman
Mariano
M
Díez
Javier
J
D000368:Aged; D017304:Annexin A5 / Q000378:metabolism*; D017209:Apoptosis; D015153:Blotting, Western; D003430:Cross-Sectional Studies; D005260:Female; D006331:Heart Diseases / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D006973:Hypertension / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism*; D010949:Plasma; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013599:Systole / Q000502:physiology; D015854:Up-Regulation
D017304:Annexin A5