title: Gene transfer into porcine myocardium via pericardial cavity by homemade easy puncture device.
abstract: OBJECTIVE
  To explore the feasibility and safety of gene transfer into porcine myocardium via the pericardial cavity by a homemade easy device.
  
  
  METHODS
  Replication-deficient recombinant adenoviral vector carrying LacZ report gene (Ad-LacZ) was constructed by the calcium phosphate precipitation method. Twelve healthy Chinese mini-swine were randomly divided into experimental group (n = 6) and control group (n = 6). Acute myocardial infarction (AMI) model was established by balloon occlusion of the distal part of D1 branch of left anterior descending (LAD) artery, at the same time the intrapericardial cavity injections were performed through the small incision of the abdominal wall below the xyphoid appendix using a homemade device. Then gene transfer was performed using a central venous catheter. The pericardium was pretreated with injection of a mixture of collagenase (1,200 U) and hyaluronidase (3,000 U) in both groups. Then 2.0 x 10(9) plaque formation unit (PFU) Ad-LacZ was injected into the pericardial cavity in experimental group, while 1 mL of normal saline was injected in the control group. The beta-galactosidase activity detection and X-gal staining of the ischemic myocardium were performed on the 3rd, 7th, and 28th day after injection.
  
  
  RESULTS
  The LAD artery was occluded completely and infarction and ischemia were detected by histological assessment In experimental group, the X-gal staining positive cells and beta-galactosidase activity quantification were detectable on the 3rd day after injection, increased markedly on the 7th day, and then declined on the 28th day. The transfer efficiencies indicated by the positive myocardial cells were 16.7%, 45.6% , 22.8% on the 3rd, 7th, 28th day, respectively. In control group, no positive cells and beta-galactosidase activity were observed.
  
  
  CONCLUSIONS
  Adenovirus can be transferred into ischemic myocardium and express target gene in the AMI model for four weeks with the homemade easy device via pericardial cavity pretreated by collagenase and hyaluronidase.
authors.affiliation: Department of Cardiology, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
authors.forename: Huai-Min
authors.initials: HM
authors.lastname: Guan
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Jin-Hong
authors.initials: JH
authors.lastname: Xie
authors.forename: Feng-Ling
authors.initials: FL
authors.lastname: Wang
authors.forename: Lin-Sheng
authors.initials: LS
authors.lastname: Cao
authors.forename: Qi-Jun
authors.initials: QJ
authors.lastname: Qian
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D006321:Heart; D007763:Lac Operon; D009206:Myocardium / Q000201:enzymology; D010496:Pericardium / Q000502:physiology*; D011677:Punctures / Q000379:methods*; D013552:Swine; D013556:Swine, Miniature; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D001616:beta-Galactosidase