Unique lipid a modifications in Pseudomonas aeruginosa isolated from the airways of patients with cystic fibrosis.
Three structural features of lipid A (addition of palmitate [C16 fatty acid], addition of aminoarabinose [positively charged amino sugar residue], and retention of 3-hydroxydecanoate [3-OH C10 fatty acid]) were determined for Pseudomonas aeruginosa isolates from patients with cystic fibrosis (CF; n=86), from the environment (n=13), and from patients with other conditions (n=14). Among P. aeruginosa CF isolates, 100% had lipid A with palmitate, 24.6% with aminoarabinose, and 33.3% retained 3-hydroxydecanoate. None of the isolates from the environment or from patients with other conditions displayed these modifications. These results indicate that unique lipid A modifications occur in clinical P. aeruginosa CF isolates.
Ernst
Robert K
RK
Department of Medicine, University of Washington, Seattle, WA 98195, USA. rkernst@u.washington.edu
Moskowitz
Samuel M
SM
Emerson
Julia C
JC
Kraig
Gretchen M
GM
Adams
Kristin N
KN
Harvey
Megan D
MD
Ramsey
Bonnie
B
Speert
David P
DP
Burns
Jane L
JL
Miller
Samuel I
SI
D001089:Arabinose / Q000031:analogs & derivatives / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D003550:Cystic Fibrosis / Q000453:epidemiology / Q000382:microbiology*; D003652:Decanoic Acids / Q000032:analysis; D006801:Humans; D007223:Infant; D008050:Lipid A / Q000737:chemistry*; D008171:Lung Diseases / Q000453:epidemiology / Q000382:microbiology*; D010168:Palmitates / Q000032:analysis; D015995:Prevalence; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000145:classification* / Q000302:isolation & purification / Q000472:pathogenicity
D003652:Decanoic Acids; D008050:Lipid A; D010168:Palmitates; C016982:aminoarabinose; D001089:Arabinose; C017552:myrmicacin