title: Determination of the cross-reactivities for alpha-zearalenol, beta-zearalenol, zearalanone, alpha-zearalanol, and beta-zearalanol on three commercial immunoaffinity columns targeting zearalenone.
abstract: Immunoaffinity extraction has become increasingly important as a sample preparation and cleanup method in mycotoxin analysis. In this study, the antibody specificities of 3 commercial immunoaffinity columns (IACs) targeting zearalenone (ZON) were compared for alpha-zearalenol, beta-zearalenol, zearalanone, alpha-zearalanol, and beta-zearalanol. The recoveries of ZON and its 5 analogs were determined in triplicate when extracted from 10 mL circumneutral river water samples spiked with 20 ng analyte individually or in a mixture. The analytes were analyzed by means of electrospray ionization liquid chromatography/tandem mass spectrometry using deuterated internal standards for quantitation. Recoveries ranged from 69 to 115% for all analytes with relative standard deviations of 1-39%. Cross-reactivities for the analogs were > 80% when applied both individually and in a mixture. No significant competition effects were observed when the compounds were applied as a multianalyte mixture well below the stated IAC capacities. The results obtained here demonstrate that all IACs tested are highly cross-reactive towards the 5 ZON derivatives and may be applied for their simultaneous extraction or cleanup.
authors.affiliation: Agroscope Reckenholz-Tänikon Research Station ART, Reckenholzstrasse 191, CH-8046 Zurich, Switzerland.
authors.forename: Marianne
authors.initials: M
authors.lastname: Erbs
authors.forename: Niccolo
authors.initials: N
authors.lastname: Hartmann
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Bucheli
mesh_terms: D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002853:Chromatography, Liquid; D004968:Estrogens, Non-Steroidal / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D013058:Mass Spectrometry; D008956:Models, Chemical; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D015025:Zearalenone / Q000031:analogs & derivatives* / Q000032:analysis*; D015029:Zeranol / Q000031:analogs & derivatives* / Q000032:analysis*
chemical_list: D004968:Estrogens, Non-Steroidal; C029659:zearalenol; D015025:Zearalenone; D015029:Zeranol; C028226:taleranol