title: Factor XI/ADAMTS13 complexes are quantitatively insignificant in human plasma.
abstract: Reportedly, complexes between factor XI and ADAMTS13 are detected with a commercial ADAMTS13/FXI ELISA kit in plasma and are decreased in thrombotic thrombocytopenic purpura (TTP). Using this kit, control and TTP patient plasma contained varying amounts of signal (25-670% of a reference plasma) but no signal was observed for mixtures of recombinant enzymes, suggesting little interaction. ADAMTS13/FXI complexes were undetectable by immunoprecipitation or gel filtration chromatography in control plasma or mixtures of recombinant proteins. These results suggest that ADAMTS13/FXI complexes are insignificant in plasma and unlikely to affect the function of either protein during normal hemostasis or in TTP.
authors.affiliation: Department of Medicine, Washington University School of Medicine, 660 S. Euclid, Box 8066, St. Louis, MO 63110, USA. tanderso@im.wustl.edu
authors.forename: Patricia J
authors.initials: PJ
authors.lastname: Anderson
authors.forename: David
authors.initials: D
authors.lastname: Gailani
authors.forename: Hendrik B
authors.initials: HB
authors.lastname: Feys
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Gao
authors.forename: Elaine M
authors.initials: EM
authors.lastname: Majerus
authors.forename: Karen
authors.initials: K
authors.lastname: Vanhoorelbeke
authors.forename: J Evan
authors.initials: JE
authors.lastname: Sadler
mesh_terms: D051722:ADAM Proteins / Q000097:blood*; D000071120:ADAMTS13 Protein; D002850:Chromatography, Gel; D005172:Factor XI / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding
chemical_list: D005172:Factor XI; D051722:ADAM Proteins; D000071120:ADAMTS13 Protein; C099604:ADAMTS13 protein, human