title: Neonatal dietary supplementation of arachidonic acid increases prostaglandin levels in adipose tissue but does not promote fat mass development in guinea pigs.
abstract: The role of arachidonic acid (AA) on the development of adipose tissue is still controversial since its metabolites, i.e., prostaglandins, can either stimulate or inhibit preadipocyte differentiation in vitro. In the present study, we evaluated the effects of early postnatal supplementation of AA on body weight and adipose tissue development in guinea pigs. Male newborn guinea pigs were fed for 21 days (day 21) with diets (milk and pellet) supplemented (+AA) or not (-AA) with 1.2% (total fatty acids) AA. From day 21 to day 105 both groups were fed a chow diet. The 21-days-old +AA pups showed a twofold higher AA accretion in phospholipids associated with a two- to sixfold increase in several prostaglandins, such as 6-keto PGF(1alpha) (the stable hydrolysis product of PGI(2)), PGF(2alpha), PGE(2), and PGD(2) in adipose tissue, compared with the -AA group. No difference in fat pad and body weight, aP2, and leptin gene expression in adipose tissue, fasting plasma glucose, free-fatty acids, and triglyceride concentration was observed between groups at day 21 or day 105. These results show that dietary supplementation of AA during the suckling/weaning period increases prostaglandin levels in adipose tissue but does not influence early fat mass development in the guinea pig.
authors.affiliation: Nestlé Research Centre, PO Box 44, Vers-Chez-Les-Blanc, 1000 Lausanne 26, Switzerland.
authors.forename: Olivier
authors.initials: O
authors.lastname: Aprikian
authors.forename: Denis
authors.initials: D
authors.lastname: Reynaud
authors.forename: Cecil
authors.initials: C
authors.lastname: Pace-Asciak
authors.forename: Patricia
authors.initials: P
authors.lastname: Leone
authors.forename: Florence
authors.initials: F
authors.lastname: Blancher
authors.forename: Irina
authors.initials: I
authors.lastname: Monnard
authors.forename: Christian
authors.initials: C
authors.lastname: Darimont
authors.forename: Katherine
authors.initials: K
authors.lastname: Macé
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D050154:Adiposity / Q000187:drug effects*; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D000833:Animals, Suckling; D016718:Arachidonic Acid / Q000494:pharmacology*; D001835:Body Weight / Q000502:physiology; D004032:Diet; D015777:Eicosanoids / Q000032:analysis; D002149:Energy Intake / Q000187:drug effects; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006168:Guinea Pigs; D020738:Leptin / Q000096:biosynthesis; D018482:Muscle, Skeletal / Q000187:drug effects / Q000254:growth & development; D011453:Prostaglandins / Q000096:biosynthesis*
chemical_list: D015777:Eicosanoids; D005227:Fatty Acids; D020738:Leptin; D011453:Prostaglandins; D016718:Arachidonic Acid