title: Crystallization and preliminary X-ray analysis of Streptococcus mutans dextran glucosidase.
abstract: Dextran glucosidase from Streptococcus mutans is an exo-hydrolase that acts on the nonreducing terminal alpha-1,6-glucosidic linkage of oligosaccharides and dextran with a high degree of transglucosylation. Based on amino-acid sequence similarity, this enzyme is classified into glycoside hydrolase family 13. Recombinant dextran glucosidase was purified and crystallized by the hanging-drop vapour-diffusion technique using polyethylene glycol 6000 as a precipitant. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 72.72, b = 86.47, c = 104.30 A. A native data set was collected to 2.2 A resolution from a single crystal.
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Saburi
authors.forename: Hironori
authors.initials: H
authors.lastname: Hondoh
authors.forename: Hideaki
authors.initials: H
authors.lastname: Unno
authors.forename: Masayuki
authors.initials: M
authors.lastname: Okuyama
authors.forename: Haruhide
authors.initials: H
authors.lastname: Mori
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Nakada
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Matsuura
authors.forename: Atsuo
authors.initials: A
authors.lastname: Kimura
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D003911:Dextrans / Q000378:metabolism; D005959:Glucosidases / Q000737:chemistry* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D013295:Streptococcus mutans / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D003911:Dextrans; D011994:Recombinant Proteins; D005959:Glucosidases; C023149:exo-1,6-alpha-glucosidase