The DUF81 protein TauE in Cupriavidus necator H16, a sulfite exporter in the metabolism of C2 sulfonates.
The degradation of taurine, isethionate and sulfoacetate in Cupriavidus necator (Ralstonia eutropha) H16 was shown by enzyme assays to be inducible, and each pathway involved sulfoacetaldehyde, which was subject to phosphatolysis by a common sulfoacetaldehyde acetyltransferase (Xsc, H16_B1870) to yield acetyl phosphate and sulfite. The neighbouring genes encoded phosphate acetyltransferase (Pta, H16_B1871) and a hypothetical protein [domain of unknown function (DUF)81, H16_B1872], with eight derived transmembrane helices. RT-PCR showed inducible transcription of these three genes, and led to the hypothesis that H16_B1872 and orthologous proteins represent a sulfite exporter, which was named TauE.
Weinitschke
Sonja
S
Department of Biology, The University, D-78457 Konstanz, Germany.
Denger
Karin
K
Department of Biology, The University, D-78457 Konstanz, Germany.
Cook
Alasdair M
AM
Department of Biology, The University, D-78457 Konstanz, Germany.
Smits
Theo H M
THM
Department of Biology, The University, D-78457 Konstanz, Germany.
D000079:Acetaldehyde / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D000476:Alkanesulfonates / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D020118:Cupriavidus necator / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D007513:Isethionic Acid / Q000737:chemistry / Q000378:metabolism; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013447:Sulfites / Q000378:metabolism*; D013654:Taurine / Q000737:chemistry / Q000378:metabolism*; D014158:Transcription, Genetic
D000476:Alkanesulfonates; D001426:Bacterial Proteins; D013447:Sulfites; D013654:Taurine; C015517:sulfoacetaldehyde; D007513:Isethionic Acid; D000079:Acetaldehyde