Differential behavioral effect of the TRPM8/TRPA1 channel agonist icilin (AG-3-5).
Molecular identification of two new transient receptor potential (TRP) channels, TRPM8 and TRPA1, has prompted an intense interest in their functional roles. We report that an acute exposure to the TRPM8/TRPA1 agonist icilin (0.01-100 microM), but not TRPV1 agonist capsaicin (10 microM), causes an atypical dose-related increase in planarian motility. This is the first demonstration of a TRPM8/TRPA1 channel subtype agonist-induced differential pharmacological effect in invertebrates and provides a novel sensitive, quantifiable end-point for studying TRP channel pharmacology.
Rawls
Scott M
SM
Department of Pharmaceutical Sciences, Temple University School of Pharmacy, Philadelphia, PA 19140, USA. scott.rawls@temple.edu
Gomez
Teresa
T
Ding
Zhe
Z
Raffa
Robert B
RB
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects* / Q000502:physiology; D015220:Calcium Channels / Q000187:drug effects* / Q000378:metabolism; D002211:Capsaicin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D010932:Planarians / Q000187:drug effects* / Q000502:physiology; D011744:Pyrimidinones / Q000494:pharmacology*; D018689:Sensory System Agents / Q000494:pharmacology; D050053:TRPM Cation Channels / Q000819:agonists* / Q000378:metabolism; D013997:Time Factors
D015220:Calcium Channels; D011744:Pyrimidinones; D018689:Sensory System Agents; D050053:TRPM Cation Channels; C490483:icilin; D002211:Capsaicin