[Methylation specific binding activity of zinc finger protein Kaiso].
OBJECTIVE
To compare binding activity of different zinc finger domain of human Kaiso with methylated CpG.


METHODS
pGEX constructs with different human Kaiso domain were generated and then corresponding fusion proteins were induced and purified. Electrophoretic mobility shift assays were applied to evaluate the binding activity of fusion proteins with methylated CpG.


RESULTS
The purified GST-KaisoZF fusion protein (without the POZ protein binding domain) could bind with methylated CpG probe specifically, but not for three or two zinc fingers without flanking domains.


CONCLUSIONS
Human zinc finger protein Kaiso could bind with methylated CpG specifically, only in the assistance of the neighboring flank sequence of the zinc finger domain.
Zhang
Bao-Zhen
BZ
Peking University School of Oncology and Beijing Institute for Cancer Research, Beijing 100036, China.
Gu
Lian-Kun
LK
Deng
Da-Jun
DJ
D001483:Base Sequence; D018899:CpG Islands; D019175:DNA Methylation; D006801:Humans; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
D014157:Transcription Factors; C492254:ZBTB33 protein, human