title: [Culture of chondrocytes using nano-hydroxyapatite/collagen in vitro].
abstract: OBJECTIVE
  To evaluate the value of the nano-hydroxyapatite/ collagen (nHAC) as a scaffold for cartilage tissue engineering.
  
  
  METHODS
  The normal cartilage from patients after total hip arthroplasty of osteoarthritis was selected, and then chondrocytes were digested, separated, and amplificate in vitro. The chondrocytes were seeded onto the nHAC scaffold and were cultured in a 3-dimensional environment for 5 and 10 days. The effects of the composite scaffold on the cell adhesion, proliferation, morphological changes, and synthesis of the extracellular matrix were observed by scanning electronic microscopy and immunohistochemistry.
  
  
  RESULTS
  The chondrocytes could adhere to the surface of the scaffolds, proliferate, and migrate into the scaffolds. They maintained round and could secrete extracellular matrix on the porous scaffold.
  
  
  CONCLUSIONS
  The nHAC can be used as the cartilage cell carrier.
authors.affiliation: Research Institute of Traumatic Orthopaedics, Second Xiangya Hospital, Central South University, Changsha 410011, China. xiaotaoxyl@163.com
authors.forename: Tao
authors.initials: T
authors.lastname: Xiao
authors.forename: Cheng
authors.initials: C
authors.lastname: Peng
authors.forename: Yuan-Ming
authors.initials: YM
authors.lastname: Luo
authors.forename: Mian-Hui
authors.initials: MH
authors.lastname: Lin
authors.forename: Xia-Jun
authors.initials: XJ
authors.lastname: Liu
mesh_terms: D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D003094:Collagen; D017886:Durapatite; D006801:Humans; D019736:Prostheses and Implants; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: C509317:nano-hydroxyapatite-collagen; D003094:Collagen; D017886:Durapatite