Altered detection of molecules associated with leukocyte traffic in HUVECs derived from newborns with a strong family history of myocardial infarction.
Atherosclerosis is a chronic inflammatory disease. As such, recruitment of immune cells is a significant event. Tightly controlled signaling molecules regulate leukocyte adhesion and migration to the tissues. The aim of this study was to determine if human umbilical vein endothelial cells (HUVECs) derived from healthy newborns with a strong family history of myocardial infarction (FHMI) showed variations in the presence of molecules related with leukocyte traffic and migration, in comparison to control healthy newborns. For this purpose, we evaluated the labeling of sialic acid containing glycoproteins, tight junction claudins and the cytoskeleton, using lectin- and immunocytochemistry in HUVECs from individuals with and without a strong FHMI. Our results show important differences in the labeling of alpha-2,3 or alpha-2,6 sialic acid-containing glycoconjugates, a disarrangement of actin filaments secondary to the absence of cytoplasmic claudin-5 immunopositivity and an increase in the binding of FHMI HUVECs to CD3+ Jurkat cells. It is possible that these differences relate to a predisposition for early appearance of atherosclerotic lesions.
Paez
Araceli
A
Departamento Biología Celular, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Col. Seccion 16, México 14080, México.
Rodriguez
Emma
E
Rendon
Erika
E
Varela
Elvira
E
Fortoul
Teresa
T
Espinosa
Blanca
B
Masso
Felipe
F
Guevara
Jorge
J
Montaño
Luis F
LF
D000328:Adult; D050197:Atherosclerosis / Q000378:metabolism / Q000473:pathology; D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D062507:Claudin-5; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D019169:Jurkat Cells; D037102:Lectins / Q000737:chemistry; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D011247:Pregnancy; D014471:Umbilical Veins / Q000166:cytology
C107466:CLDN5 protein, human; D062507:Claudin-5; D037102:Lectins; D008565:Membrane Proteins; D019158:N-Acetylneuraminic Acid