title: Rat insulin promoter 2-Cre recombinase mice bred onto a pure C57BL/6J background exhibit unaltered glucose tolerance.
abstract: Beta-cell-specific gene targeting is a widely used tool when studying genes involved in beta-cell function. For this purpose, several conditional beta-cell knockouts have been generated using the rat insulin promoter 2-Cre recombinase (RIP2-Cre) mouse. However, it was recently observed that expression of Cre alone in beta-cells may affect whole body glucose homeostasis. Therefore, we investigated glucose homeostasis, insulin secretion, and beta-cell mass in our line of RIP2-Cre mice bred onto the C57BL/6J genetic background. We used 12- and 28-week-old female RIP2-Cre mice for analyses of insulin secretion in vitro, glucose homeostasis in vivo and beta-cell mass. Our mouse line has been backcrossed for 14 generations to yield a near 100% pure C57BL/6J background. We found that fasting plasma glucose and insulin levels were similar in both genotypes. An i.v. glucose tolerance test revealed no differences in glucose clearance and insulin secretion between 12-week-old RIP2-Cre and WT mice. Moreover, insulin secretion in vitro in islets isolated from 28-week-old RIP2-Cre mice and controls was similar. In addition, beta-cell mass was not different between the two genotypes at 28 weeks of age. In our experiments, we observed no differences in glucose tolerance, insulin secretion in vivo and in vitro, or in beta-cell mass between the genotypes. As our RIP2-Cre mice are on a near 100% pure genetic background (C57BL/6J), we suggest that the perturbations in glucose homeostasis previously reported in RIP2-Cre mouse lines can be accounted for by differences in genetic background.
authors.affiliation: Department of Experimental Medical Science, Lund University, SE-22184 Lund, Sweden. malin.fex@med.lu.se
authors.forename: Malin
authors.initials: M
authors.lastname: Fex
authors.forename: Nils
authors.initials: N
authors.lastname: Wierup
authors.forename: Marloes Dekker
authors.initials: MD
authors.lastname: Nitert
authors.forename: Michael
authors.initials: M
authors.lastname: Ristow
authors.forename: Hindrik
authors.initials: H
authors.lastname: Mulder
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis / Q000378:metabolism*; D001947:Breeding; D005260:Female; D005838:Genotype; D005951:Glucose Tolerance Test; D007031:Hypothalamus / Q000378:metabolism; D007150:Immunohistochemistry; D007328:Insulin / Q000097:blood / Q000378:metabolism; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000378:metabolism; D019426:Integrases / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D023421:Models, Animal; D010641:Phenotype; D011401:Promoter Regions, Genetic; D051381:Rats; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2
chemical_list: D001786:Blood Glucose; D007328:Insulin; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2; C045073:Cre recombinase; D019426:Integrases