title: Unique lipid a modifications in Pseudomonas aeruginosa isolated from the airways of patients with cystic fibrosis.
abstract: Three structural features of lipid A (addition of palmitate [C16 fatty acid], addition of aminoarabinose [positively charged amino sugar residue], and retention of 3-hydroxydecanoate [3-OH C10 fatty acid]) were determined for Pseudomonas aeruginosa isolates from patients with cystic fibrosis (CF; n=86), from the environment (n=13), and from patients with other conditions (n=14). Among P. aeruginosa CF isolates, 100% had lipid A with palmitate, 24.6% with aminoarabinose, and 33.3% retained 3-hydroxydecanoate. None of the isolates from the environment or from patients with other conditions displayed these modifications. These results indicate that unique lipid A modifications occur in clinical P. aeruginosa CF isolates.
authors.affiliation: Department of Medicine, University of Washington, Seattle, WA 98195, USA. rkernst@u.washington.edu
authors.forename: Robert K
authors.initials: RK
authors.lastname: Ernst
authors.forename: Samuel M
authors.initials: SM
authors.lastname: Moskowitz
authors.forename: Julia C
authors.initials: JC
authors.lastname: Emerson
authors.forename: Gretchen M
authors.initials: GM
authors.lastname: Kraig
authors.forename: Kristin N
authors.initials: KN
authors.lastname: Adams
authors.forename: Megan D
authors.initials: MD
authors.lastname: Harvey
authors.forename: Bonnie
authors.initials: B
authors.lastname: Ramsey
authors.forename: David P
authors.initials: DP
authors.lastname: Speert
authors.forename: Jane L
authors.initials: JL
authors.lastname: Burns
authors.forename: Samuel I
authors.initials: SI
authors.lastname: Miller
mesh_terms: D001089:Arabinose / Q000031:analogs & derivatives / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D003550:Cystic Fibrosis / Q000453:epidemiology / Q000382:microbiology*; D003652:Decanoic Acids / Q000032:analysis; D006801:Humans; D007223:Infant; D008050:Lipid A / Q000737:chemistry*; D008171:Lung Diseases / Q000453:epidemiology / Q000382:microbiology*; D010168:Palmitates / Q000032:analysis; D015995:Prevalence; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000145:classification* / Q000302:isolation & purification / Q000472:pathogenicity
chemical_list: D003652:Decanoic Acids; D008050:Lipid A; D010168:Palmitates; C016982:aminoarabinose; D001089:Arabinose; C017552:myrmicacin