Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Förster distance.
Transthyretin (TTR) is an amyloidogenic protein involved in many mental diseases. The peptide derived from TTR (105-115) has been widely studied as a model peptide for understanding the mechanism of amyloid fibril formation. However, the detailed arrangement of this peptide in amyloid fibril is still unclear. We have studied the amyloid fibril formation process of TTR (105-115) by introducing a pair of FRET probes into the peptide with a dansyl group at the N-terminal and a tryptophan residue at the C-terminal. Our experiment demonstrated that the strands of TTR (105-115) in the same beta-sheet may be parallel and the mating sheets may be anti-parallel to each other in the amyloid fibril. The kinetics followed by FRET and EM indicated for a possible intermediate state and the distance between sheets became shorter when the intermediate amyloid fibril turns into a more matured form.
Deng
Wei
W
Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Structural Chemistry for Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Cao
Aoneng
A
Lai
Luhua
L
D000682:Amyloid / Q000737:chemistry*; D000686:Amyloidosis / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D010455:Peptides / Q000737:chemistry*; D011228:Prealbumin / Q000737:chemistry*; D011487:Protein Conformation; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013053:Spectrophotometry / Q000379:methods
D000682:Amyloid; D010455:Peptides; D011228:Prealbumin