Detection of CTX-M-14 extended-spectrum beta-lactamase in Shigella sonnei isolates from China.
Shigellosis is an important cause of acute diarrheal disease and multidrug-resistant phenotype has been reported in S. sonnei. In this study, we investigate the resistance and identify extended-spectrum beta-lactamases (ESBLs) gene in 37 S. sonnei isolates by agar dilution procedure and the modified three-dimensional test, respectively. The bla genes of ESBL-producing isolates were detected by polymerase chain reaction (PCR) and sequencing. More than 50% of these strains were resistant to tetracycline, sulfamethoxazole-trimethoprim, ampicillin, ampicillin-sulbactam, or gentamicin. However, they were still susceptible to third generation cephalosporins, fluoroquinolones, and chloramphenicol. A total of 8.1% (3/37) of the isolates with intermediate susceptibility to ceftriaxone and cefotaxime were ESBL-producers, which produced CTX-M-14 ESBLs and TEM-1 beta-lactamases. This is the first report of CTX-M-14 in S. sonnei isolates from China and it is important to closely monitor such strains.
Xiong
Zizhong
Z
Department of Infectious Diseases, The First Affiliated Hospital, Anhui Medical University, Hefei 230022, China.
Li
Tao
T
Xu
Yuanhong
Y
Li
Jun
J
D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D004405:Dysentery, Bacillary / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012764:Shigella sonnei / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
D004269:DNA, Bacterial; C456080:beta-lactamase CTX-M-14; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1