title: Characterization of a new HLA-B allele, HLA-B*5312, and re-evaluation of the published sequences of the untranslated regions of HLA-B*35 and HLA-B*53.
abstract: We report a novel human leukocyte antigen (HLA)-B allele, HLA-B*5312. Compared with HLA-B*530101, there is one silent substitution at nucleotide 438 and two non-synonymous substitutions at nucleotides 431 and 440, causing a change of the amino acid sequence (Asn-->Ser at codon 77 and Ile-->Thr at codon 80, respectively) within the Bw4 epitope. In contrast to the published sequences (IMGT/HLA Database, version 2.16.0, January 2007), we found that HLA-B*530101 had a C instead of a T at nucleotide -221, whereas HLA-B*350101 had a C instead of an A at nucleotide 2992. According to our sequencing results, HLA-B*5312 resembles HLA-B*350101 regarding its sequence of the untranslated regions. HLA-B*5312 may have been the result of a double crossing over event during which HLA-B*350101 adopted a Bw4 motif.
authors.affiliation: Department of Transplantation Immunology, Institute of Immunology, University of Heidelberg, Im Neuenheimer Feld 305, 69120 Heidelberg, Germany. hien.tran@med.uni-heidelberg.de
authors.forename: A
authors.initials: A
authors.lastname: Heinold
authors.forename: M
authors.initials: M
authors.lastname: Bauer
authors.forename: S
authors.initials: S
authors.lastname: Scherer
authors.forename: G
authors.initials: G
authors.lastname: Opelz
authors.forename: T H
authors.initials: TH
authors.lastname: Tran
mesh_terms: D000483:Alleles; D001483:Base Sequence; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D020506:Untranslated Regions
chemical_list: D015235:HLA-B Antigens; D020506:Untranslated Regions