Branched KLVFF tetramers strongly potentiate inhibition of beta-amyloid aggregation.
The key pathogenic event in the onset of Alzheimer's disease (AD) is the aggregation of beta-amyloid (Abeta) peptides into toxic aggregates. Molecules that interfere with this process might act as therapeutic agents for the treatment of AD. The amino acid residues 16-20 (KLVFF) are known to be essential for the aggregation of Abeta. In this study, we have used a first-generation dendrimer as a scaffold for the multivalent display of the KLVFF peptide. The effect of four KLVFF peptides attached to the dendrimer (K(4)) on Abeta aggregation was compared to the effect of monomeric KLVFF (K(1)). Our data show that K(4) very effectively inhibits the aggregation of low-molecular-weight and protofibrillar Abeta(1-42) into fibrils, in a concentration-dependent manner, and much more potently than K(1). Moreover, we show that K(4) can lead to the disassembly of existing aggregates. Our data lead us to propose that conjugates that bear multiple copies of KLVFF might be useful as therapeutic agents for the treatment of Alzheimer's disease.
Chafekar
Sidhartha M
SM
Neurogenetics Laboratory, Academic Medical Center, P.O. Box 22660, 1100 DD Amsterdam, The Netherlands.
Malda
Hinke
H
Merkx
Maarten
M
Meijer
E W
EW
Viertl
David
D
Lashuel
Hilal A
HA
Baas
Frank
F
Scheper
Wiep
W
D000544:Alzheimer Disease / Q000188:drug therapy* / Q000473:pathology; D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000378:metabolism; D050091:Dendrimers / Q000737:chemistry / Q000494:pharmacology; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*
D000682:Amyloid; D016229:Amyloid beta-Peptides; D050091:Dendrimers; C478940:Lys-Leu-Val-Phe-Phe-Lys-Lys-Lys-Lys-Lys-Lys; D009842:Oligopeptides