A Polysaccharide from tomato (Lycopersicon esculentum) peels affects NF-kappaB activation in LPS-stimulated J774 macrophages.
We investigated the effect of PS ( 1) on nitrite and reactive oxygen species (ROS) production in J774 macrophages stimulated with bacterial lipopolysaccharide (LPS) for 24 h. PS ( 1) inhibited in a concentration-dependent manner nitrite and ROS production as well as inducible nitric oxide synthase (iNOS) protein expression induced by LPS. Incubation of cells with PS ( 1) determined a significant decrease of nuclear factor-kappaB (NF-kappaB)/DNA binding activity which was correlated with a marked reduction of iNOS mRNA levels. These results show that PS ( 1) inhibits NF-kappaB activation and iNOS gene expression by preventing the reactive species production and suggest a role for this compound in controlling oxidative stress and/or inflammation.
De Stefano
Daniela
D
Dipartimento di Farmacologia Sperimentale, Facoltà di Scienze Biotecnologiche, Università degli Studi di Napoli Federico II, Via D. Montesano, 49, 80131 Napoli, Italy. dadestef@unina.it
Tommonaro
Giuseppina
G
Simeon
Vittorio
V
Poli
Annarita
A
Nicolaus
Barbara
B
Carnuccio
Rosa
R
D000818:Animals; D015153:Blotting, Western; D024202:Electrophoretic Mobility Shift Assay; D008070:Lipopolysaccharides / Q000494:pharmacology*; D018551:Solanum lycopersicum / Q000737:chemistry*; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D008070:Lipopolysaccharides; D016328:NF-kappa B; D011134:Polysaccharides; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II