title: Humoral responses to oxidized low-density lipoprotein and related bacterial antigens after pneumococcal vaccine.
abstract: Antibodies to oxidized low-density lipoprotein (oxLDL) may modulate the development of atherosclerosis. Antibodies to oxLDL may also react with cell wall polysaccharides (CWPS) of Streptococcus pneumoniae because both antigens share a common phosphorylcholine moiety. In hypercholesteremic mice, immunization with pneumococcal organisms elicited antibodies to oxLDL and protection against atherosclerosis. In humans, we determined whether the widely used adult pneumococcal polysaccharide vaccine augmented antibodies to oxLDL, CWPS, and phosphorylcholine, providing the potential to retard atherogenesis. Before and 4 weeks after pneumococcal vaccination of 23 healthy adults (11 smokers and 12 matched nonsmokers), we characterized IgG, IgM, and IgA to pneumococcal capsular polysaccharides, CWPS, and phosphorylcholine, IgG and IgM to oxLDL, and fasting serum lipids. The pneumococcal vaccine elicited significant increases in each antibody class to surface capsular polysaccharides. In contrast, only IgG to CWPS increased modestly and only among smokers. Moreover, antibodies to neither phosphorylcholine nor oxLDL increased consistently in either group. The pneumococcal polysaccharide vaccine effectively elicits antibodies to the bacterial capsule. The vaccine had no effect on serum lipids. The vaccine did not augment antibodies to CWPS, to its component phosphorylcholine, or to oxLDL, which are antibodies that have been proposed to modify the uptake of oxLDL by macrophages and the pathogenesis of atherosclerosis.
authors.affiliation: Cardiology Section, University of Minnesota School of Medicine, Veterans Affair Medical Center, Minneapolis, Minn., USA.
authors.forename: John T
authors.initials: JT
authors.lastname: Nguyen
authors.forename: Nicollette
authors.initials: N
authors.lastname: Myers
authors.forename: Jana
authors.initials: J
authors.lastname: Palaia
authors.forename: Angeliki
authors.initials: A
authors.lastname: Georgopoulos
authors.forename: Jeffrey B
authors.initials: JB
authors.lastname: Rubins
authors.forename: Edward N
authors.initials: EN
authors.lastname: Janoff
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D000918:Antibody Specificity; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007136:Immunoglobulins / Q000097:blood; D008055:Lipids / Q000097:blood; D053419:Lipoproteins, IDL / Q000097:blood* / Q000276:immunology; D008077:Lipoproteins, LDL / Q000008:administration & dosage / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D022242:Pneumococcal Vaccines / Q000276:immunology / Q000627:therapeutic use*; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D012907:Smoking / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D008055:Lipids; D053419:Lipoproteins, IDL; D008077:Lipoproteins, LDL; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial; C099252:oxidized low density lipoprotein