DAZL protein expression in mouse preimplantation embryo.
OBJECTIVE
To evaluate the expression pattern of Dazl (deleted in azoospermia-like) protein in the mouse preimplantation embryo.


METHODS
Experimental study.


METHODS
Medical research laboratory in a university hospital.


METHODS
Twenty female 28- to 35-day-old FVB mice.


METHODS
Embryo collection at 1.5, 2.5, and 3.5 days postcoitus (plug date, 0.5 d postcoitus) to examine the Dazl protein expression from the two-cell embryo to the blastocyst.


METHODS
Dazl protein expression was analyzed by immunofluorescent staining.


RESULTS
There is abundant expression of Dazl protein in the cytoplasm of the blastomere. Strong fluorescent signals of Dazl protein expression were found in preimplantation embryo cytoplasm, including two-cell, eight-cell, morula, and blastocyst.


CONCLUSIONS
By using an antibody raised against mouse Daz-like protein (Dazl), we showed that Dazl protein is present in all cleaving stages of the preimplantation embryo. This is the first report on the protein expression of a Dazl gene during embryogenesis in mice. However, further study is needed to evaluate the molecular functional role of Dazl.
Pan
Hsien-An
HA
Department of Obstetrics and Gynecology, National Cheng Kung University, Tainan, Taiwan; Institute of Clinical Medicine and College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Liao
Rui-Wen
RW
Chung
Chia-Ling
CL
Teng
Yen-Ni
YN
Lin
Yung-Ming
YM
Kuo
Pao-Lin
PL
D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002478:Cells, Cultured; D002970:Cleavage Stage, Ovum / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D016601:RNA-Binding Proteins / Q000378:metabolism*
C508515:DAZL protein, mouse; D016601:RNA-Binding Proteins