title: The dominant-negative effect of the Q218K variant of the prion protein does not require protein X.
abstract: Previous studies identified several single-point mutants of the prion protein that displayed dominant-negative effects on prion replication. The dominant-negative effect was assumed to be mediated by protein X, an as-yet-unknown cellular cofactor that is believed to be essential for prion replication. To gain insight into the mechanism that underlies the dominant-negative phenomena, we evaluated the effect of the Q218K variant of full-length recombinant prion protein (Q218K rPrP), one of the dominant-negative mutants, on cell-free polymerization of wild-type rPrP into amyloid fibrils. We found that both Q218K and wild-type (WT) rPrPs were incorporated into fibrils when incubated as a mixture; however, the yield of polymerization was substantially decreased in the presence of Q218K rPrP. Furthermore, in contrast to fibrils produced from WT rPrP, the fibrils generated in the mixture of WT and Q218K rPrPs did not acquire the proteinase K-resistant core of 16 kDa that was shown previously to encompass residues 97-230 and was similar to that of PrP(Sc). Our studies demonstrate that the Q218K variant exhibits the dominant-negative effect in cell-free conversion in the absence of protein X, and that this effect is, presumably, mediated by physical interaction between Q218K and WT rPrP during the polymerization process.
authors.affiliation: Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, Maryland 21201, USA.
authors.forename: Cheng I
authors.initials: CI
authors.lastname: Lee
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Yang
authors.forename: Veronique
authors.initials: V
authors.lastname: Perrier
authors.forename: Ilia V
authors.initials: IV
authors.lastname: Baskakov
mesh_terms: D019943:Amino Acid Substitution; D000682:Amyloid / Q000737:chemistry* / Q000648:ultrastructure; D000818:Animals; D051379:Mice; D017354:Point Mutation; D011328:Prions / Q000737:chemistry* / Q000235:genetics*; D017433:Protein Structure, Secondary; D011506:Proteins / Q000502:physiology; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D000682:Amyloid; D011328:Prions; D011506:Proteins; D011994:Recombinant Proteins