Matrix metalloproteinase (MMP)-3 polymorphism in patients with HBV related chronic liver disease.
A common and important problem in patients with chronic hepatitis B is the progression of liver fibrosis. Matrix metalloproteinases (MMPs) play an important role in the progression of liver fibrosis. Our aim of this study was to examine the association of MMP-3 polymorphism with liver cirrhosis in Korean patients with chronic hepatitis B. Genomic DNA was extracted from 127 patients with chronic hepatitis B (CHB), 92 patients with hepatitis B virus (HBV)-related liver cirrhosis (HBV-LC), and 146 healthy subjects. MMP-3 polymorphism was determined by polymerase-chain reaction-based assays, and the association with the progression of liver cirrhosis was investigated. With regard to MMP-3 polymorphism, there was no statistical difference in genotype distributions among the three groups. However, the peripheral platelet count of the 5A carriers was significantly lower than that of the 6A homozygotes in the HBV-LV group (85.0 +/- 36.9 vs. 109.8 +/- 47.0 x 10(9)/l; P = 0.02). With MMP-3 promoter polymorphism (rs3025058), a lower peripheral blood platelet count, which was related to advanced liver cirrhosis, was observed in 5A carriers. Therefore, more studies of MMP-3 gene polymorphism with larger populations should be conducted to further understand its role in the progression of liver cirrhosis.
Shin
Hyun Phil
HP
Department of Internal Medicine, East-West Neo Medical Center, Kyung Hee University College of Medicine, Gangdong-gu, Seoul 134-090, Korea.
Lee
Joung Il
JI
Jung
Joo-Ho
JH
Yim
Sung-Vin
SV
Kim
Hyun Jeong
HJ
Cha
Jae Myung
JM
Park
Jong Beom
JB
Joo
Kwang Ro
KR
Hwang
Jae Seok
JS
Jang
Byoung-Kuk
BK
D000328:Adult; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D018450:Disease Progression; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes; D019694:Hepatitis B, Chronic / Q000150:complications*; D006801:Humans; D007723:Korea; D008103:Liver Cirrhosis / Q000235:genetics*; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
D019278:Matrix Metalloproteinase 3