title: Human beta defensin-1 and -2 expression in the gingiva of patients with specific periodontal diseases.
abstract: OBJECTIVE
  beta defensin antimicrobial peptides are important in epithelial innate immunity, and their differential expression is associated with periodontal diseases. The aims of this study were to determine the mRNA expression of human beta defensin-1 and -2 in the gingival tissue of patients with gingivitis, aggressive periodontitis and chronic periodontitis, and to evaluate the relationship between defensin expression and type and/or severity of periodontal destruction.
  
  
  METHODS
  Fifteen patients in each group with gingivitis, aggressive periodontitis and chronic periodontitis, and 10 healthy subjects, were included in the study (n=55). The periodontal status of the subjects was determined by periodontal clinical measurements and radiographical evaluations. Transcriptional levels of human beta defensin-1 and -2 genes in gingival samples were assessed by using the quantitative real-time polymerase chain reaction technique, and the data were evaluated statistically by the relative expression Software Tool 2 for groupwise comparisons.
  
  
  RESULTS
  Expression of the human beta defensin-1 gene was lower in gingivitis and aggressive periodontitis groups, and significantly higher in the chronic periodontitis group, than in the control group (p<0.001). Human beta defensin-2 mRNA expression in the gingivitis group was lower than in the control group; however, the difference was statistically significant only in half of the gingivitis patients (p<0.001). Human beta defensin-2 mRNA levels were higher in some chronic periodontitis patients, but lower in the others when compared with the control group (p<0.001). Expression of the human beta defensin-2 gene increased in the aggressive periodontitis group relative to the control group.
  
  
  CONCLUSIONS
  This study suggests that human beta defensin-1 and -2 genes in the gingival epithelium show differential expression in patients with specific periodontal diseases, and aggressive and chronic periodontitis types demonstrate different gingival beta defensin-1 and -2 expression patterns.
authors.affiliation: Ege University, School of Dentistry, Department of Periodontology, Izmir, Turkey. saynur.vardar@ege.edu.tr
authors.forename: S
authors.initials: S
authors.lastname: Vardar-Sengul
authors.forename: T
authors.initials: T
authors.lastname: Demirci
authors.forename: B H
authors.initials: BH
authors.lastname: Sen
authors.forename: V
authors.initials: V
authors.lastname: Erkizan
authors.forename: E
authors.initials: E
authors.lastname: Kurulgan
authors.forename: H
authors.initials: H
authors.lastname: Baylas
mesh_terms: D000328:Adult; D000890:Anti-Infective Agents / Q000032:analysis*; D002908:Chronic Disease; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D005881:Gingiva / Q000378:metabolism*; D005891:Gingivitis / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000145:classification / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic / Q000235:genetics; D023083:beta-Defensins / Q000032:analysis* / Q000235:genetics
chemical_list: D000890:Anti-Infective Agents; C406407:DEFB1 protein, human; C106641:DEFB4A protein, human; D012333:RNA, Messenger; D023083:beta-Defensins