title: Immobilization of Candida rugosa lipase on poly(allyl glycidyl ether-co-ethylene glycol dimethacrylate) macroporous polymer particles.
abstract: Macroporous polymer particles containing surface epoxy groups were synthesized for immobilization of Candida rugosa lipase (CRL). The effect of incorporation of two different sets of monomers [allyl glycidyl ether (AGE) and glycidyl methacrylate (GMA)] and the effect of crosslinking density on immobilization of lipase were studied. AGE-co-EGDM polymers gave higher binding and expression of lipase than GMA-co-EGDM polymers. Optimization of immobilization parameters was done with respect to immobilization time and enzyme loading. Amongst AGE-co-EGDM polymer series, AGE-150 polymer found to give maximum lipase activity yield and therefore evaluated for temperature, pH and storage stability. Under optimum conditions, AGE-150 polymer gave 78.40% of activity yield. Immobilized lipase on AGE-150 showed a broader pH, higher temperature and excellent storage stability.
authors.affiliation: Chemical Engineering and Process Development Division, National Chemical Laboratory, Pune 411 008, India.
authors.forename: Bhalchandra K
authors.initials: BK
authors.lastname: Vaidya
authors.forename: Ganesh C
authors.initials: GC
authors.lastname: Ingavle
authors.forename: S
authors.initials: S
authors.lastname: Ponrathnam
authors.forename: B D
authors.initials: BD
authors.lastname: Kulkarni
authors.forename: Sanjay N
authors.initials: SN
authors.lastname: Nene
mesh_terms: D002175:Candida / Q000187:drug effects / Q000201:enzymology*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism*; D008689:Methacrylates / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism*; D016062:Porosity / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D004852:Epoxy Compounds; D008689:Methacrylates; D011108:Polymers; C004919:ethylene dimethacrylate; D008049:Lipase; C054361:allyl glycidyl ether