title: Genome sequence comparison and superinfection between two related Pseudomonas aeruginosa phages, D3112 and MP22.
abstract: A temperate transposable bacteriophage (MP22) was isolated from a Korean clinical isolate of Pseudomonas aeruginosa. It has a coliphage lambda-like morphology and a double-stranded DNA genome. The complete nucleotide sequence and annotation of the MP22 genome and its characteristics are presented. The MP22 genome is 36 409 bp long with a G+C content of 64.2 mol%. The genome contains 51 proposed ORFs, of which 48 (94 %) display synteny and significant nucleotide and protein sequence similarity to the corresponding ORFs of the closely related phage, D3112. Three of the predicted ORFs are unique proteins, whose functions are yet to be revealed. The phage c repressors exhibit striking dissimilarities and, when present as a single gene, did not show cross-immunity. In contrast, although an MP22 lysogen could be productively infected with D3112, MP22 could not grow on a D3112 lysogen, indicating a role of other D3112 genes in superinfection exclusion.
authors.affiliation: Department of Life Science, Sogang University, Seoul 121-742, Korea.
authors.forename: Yun-Jeong
authors.initials: YJ
authors.lastname: Heo
authors.affiliation: Department of Life Science, Sogang University, Seoul 121-742, Korea.
authors.forename: In-Young
authors.initials: IY
authors.lastname: Chung
authors.affiliation: Department of Life Science, Sogang University, Seoul 121-742, Korea.
authors.forename: Kelly B
authors.initials: KB
authors.lastname: Choi
authors.affiliation: Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL 61802, USA.
authors.forename: Gee W
authors.initials: GW
authors.lastname: Lau
authors.affiliation: Department of Life Science, Sogang University, Seoul 121-742, Korea.
authors.forename: You-Hee
authors.initials: YH
authors.lastname: Cho
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics; D006801:Humans; D008242:Lysogeny; D008969:Molecular Sequence Data; D017105:Pseudomonas Phages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity / Q000821:virology*; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D012097:Repressor Proteins; D014764:Viral Proteins; C412549:lysogenic c repressor, Bacteriophage D3112