Preliminary X-ray analysis of cellobiohydrolase Cel7B from Melanocarpus albomyces.
Cellobiohydrolases are enzymes that cleave off cellobiose units from cellulose chains in a processive manner. Melanocarpus albomyces Cel7B is a thermostable single-module cellobiohydrolase that has relatively low activity on small soluble substrates at room temperature. It belongs to glycoside hydrolase family 7, which includes endo-beta-1,4-glucanases and cellobiohydrolases. Cel7B was crystallized using the hanging-drop vapour-diffusion method and streak-seeding. The crystals belonged to space group P2(1), with unit-cell parameters a = 50.9, b = 94.5, c = 189.8 A, beta = 90.0 degrees and four monomers in the asymmetric unit. Analysis of the intensity statistics showed that the crystals were pseudo-merohedrally twinned, with a twinning fraction of 0.37. X-ray diffraction data were collected at 1.6 A resolution using synchrotron radiation.
Parkkinen
Tarja
T
Department of Chemistry, University of Joensuu, PO Box 111, 80101 Joensuu, Finland. tarja.parkkinen@joensuu.fi
Koivula
Anu
A
Vehmaanperä
Jari
J
Rouvinen
Juha
J
D001203:Ascomycota / Q000201:enzymology*; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D003460:Crystallization; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D007700:Kinetics; D012542:Scattering, Radiation; D014961:X-Ray Diffraction
D005656:Fungal Proteins; D043366:Cellulose 1,4-beta-Cellobiosidase