title: [Expression profile of heat shock proteins in tissues and cells of lung adenocarcinoma].
abstract: OBJECTIVE
  To observe the expression profile of heat shock proteins (HSPs) including HSP70, inducible HSP90 (HSP86) and aB-crystallin in cells and tissues of lung adenocarcinoma.
  
  
  METHODS
  Western blotting and reverse transcriptional-polymerase chain reaction (RT-PCR) were performed to detect the expression of HSP70, HSP86 and aB-crystallin both in the protein and mRNA level respectively.
  
  
  RESULTS
  Compared with normal lung tissue and human bronchial epithelium (HBE) cells, RT-PCR and Western blotting showed that the expression of HSP70, HSP86 and alphaB crystallin increased significantly in both the mRNA and protein level in the cancer tissue and A549 human lung adenocarcinoma cells. Among the 3 sub-families of HSPs, the expression of HSP70 mRNA and protein increased most in both the lung tissue of cancer and A549 human adenocarcinoma cell lines.
  
  
  CONCLUSIONS
  The expression of HSPs is higher in the lung adenocarcinoma and A549 cells than that in the normal lung tissues and HBE cells. Among the HSP family, HSP70 is the most up-regulated member in the tissue and cells of lung adenocarcinoma.
authors.affiliation: Department of Oncology, Second Xiangya Hospital, Central South University, Changsha 410011, China. liuxianling3180@yahoo.com.cn
authors.forename: Xian-Ling
authors.initials: XL
authors.lastname: Liu
authors.forename: Kai-Ping
authors.initials: KP
authors.lastname: Guo
authors.forename: Fang
authors.initials: F
authors.lastname: Ma
authors.forename: Gui-Yuan
authors.initials: GY
authors.lastname: Xie
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.forename: Chun-Hong
authors.initials: CH
authors.lastname: Hu
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D000077192:Adenocarcinoma of Lung; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D006360:Heat-Shock Proteins