title: A comparison of the relative antioxidant potency of L-ergothioneine and idebenone.
abstract: BACKGROUND
  L-ergothioneine (EGT) is a stable antioxidant found in food plants as well as in animal tissue undergoing relatively high levels of oxidative stress. Idebenone is a stable analog of the antioxidant coenzyme Q(10). All are potent antioxidants found in skincare products, but their relative potencies are not well described.
  
  
  OBJECTIVE
  To establish the physiological relevance of EGT by examining transcription of the EGT transporter gene OCTN-1 and production of the receptor protein in skin fibroblasts. In addition, to compare the inhibition of lipid peroxide formation by coenzyme Q(10) and EGT. Furthermore, to compare the peroxide-scavenging abilities of EGT and idebenone in both simple solution and in cell cultures exposed to ultraviolet A (UVA).
  
  
  METHODS
  OCTN-1 expression and production in cultured fibroblasts were measured through real-time reverse transcription-PCR and Western blotting, respectively. Alloxan-induced lipid peroxidation in liposomes was used to evaluate the inhibition of lipid peroxide formation. The abilities of EGT and idebenone to directly scavenge hydroxyl radicals produced by H(2)O(2 )were determined. Finally, we irradiated fibroblasts with UVA340 radiation and compared antioxidant capabilities to scavenge free radicals.
  
  
  RESULTS
  We found that OCTN-1 is expressed and readily detectable in cultured human fibroblasts. EGT was more efficient in inhibiting lipid peroxide formation than coenzyme Q(10) or idebenone. Samples treated with EGT had significantly less peroxide than those treated with idebenone 120 min after adding the antioxidants to H(2)O(2). EGT acted significantly quicker and more efficiently in capturing reactive oxygen species (ROS) after UVA340 irradiation.
  
  
  CONCLUSIONS
  EGT is a natural skin antioxidant, as evidenced by the presence of the EGT transporter in fibroblasts. EGT is a more powerful antioxidant than either coenzyme Q(10) or idebenone due to its relatively greater efficiency in directly scavenging free radicals and in protecting cells from UV-induced ROS.
authors.affiliation: AGI Dermatics, Freeport, NY, USA. kdong@agiderm.com
authors.forename: Kelly K
authors.initials: KK
authors.lastname: Dong
authors.forename: Niusha
authors.initials: N
authors.lastname: Damaghi
authors.forename: Jeannie
authors.initials: J
authors.lastname: Kibitel
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Canning
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Smiles
authors.forename: Daniel B
authors.initials: DB
authors.lastname: Yarosh
mesh_terms: D000496:Alloxan / Q000494:pharmacology; D000704:Analysis of Variance; D000975:Antioxidants / Q000494:pharmacology*; D016227:Benzoquinones / Q000494:pharmacology*; D002478:Cells, Cultured; D004880:Ergothioneine / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008081:Liposomes / Q000378:metabolism; D027701:Organic Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D010545:Peroxides / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D027981:Symporters; D014451:Ubiquinone / Q000494:pharmacology / Q000031:analogs & derivatives; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D016227:Benzoquinones; D008081:Liposomes; D027701:Organic Cation Transport Proteins; D010545:Peroxides; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C109807:SLC22A4 protein, human; D027981:Symporters; D014451:Ubiquinone; D000496:Alloxan; D004880:Ergothioneine; C036619:idebenone