Enhancement of thermal stability and inhibition of protein aggregation by osmolytic effect of hydroxyproline.
A combination of spectroscopic, calorimetric, and microscopic studies to understand the effect of hydroxyproline on the thermal stability, conformation, biological activity, and aggregation of proteins has been investigated. Significantly increased protein stability and suppression of aggregation is achieved in the presence of hydroxyproline. For example, exceptional increase in the thermal stability of lysozyme up to 26.4 degrees C and myoglobin up to 31.8 degrees C is obtained in the presence of hydroxyproline. The increased thermal stability of the proteins is observed to be accompanied with significant rise of the catalytic activity. Hydroxyproline is observed to prevent lysozyme fibril formation in vitro. Fluorescence and circular dichroism studies indicate induction of tertiary structures of the studied proteins in the presence of hydroxyproline. Preferential hydration of the native state is found to be crucial for the mechanism of protein stabilization by hydroxyproline. We compared the effect of hydroxyproline to that of proline and observed similar increase in the activity and suppression of protein aggregation. The results demonstrate the use of hydroxyproline as a protein stabilizer and in the prevention of protein aggregation and fibril formation.
Kar
Karunakar
K
Indian Institute of Technology-Bombay, Powai, Mumbai, India.
Kishore
Nand
N
D002152:Calorimetry, Differential Scanning; D002384:Catalysis; D002942:Circular Dichroism / Q000379:methods; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability; D006909:Hydroxyproline / Q000737:chemistry* / Q000494:pharmacology; D007768:Lactalbumin / Q000037:antagonists & inhibitors* / Q000737:chemistry; D008854:Microscopy, Electron / Q000379:methods; D009113:Muramidase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D009211:Myoglobin / Q000037:antagonists & inhibitors* / Q000737:chemistry; D009997:Osmotic Pressure / Q000187:drug effects; D010316:Particle Size; D011392:Proline / Q000494:pharmacology; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D012710:Serum Albumin, Bovine / Q000037:antagonists & inhibitors* / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D013329:Structure-Activity Relationship; D013500:Surface Tension; D013696:Temperature
D009211:Myoglobin; D012710:Serum Albumin, Bovine; D007768:Lactalbumin; D011392:Proline; D009113:Muramidase; D006909:Hydroxyproline