Real-time detection of DNA interactions with long-period fiber-grating-based biosensor.
Using an optical biosensor based on a dual-peak long-period fiber grating, we have demonstrated the detection of interactions between biomolecules in real time. Silanization of the grating surface was successfully realized for the covalent immobilization of probe DNA, which was subsequently hybridized with the complementary target DNA sequence. It is interesting to note that the DNA biosensor was reusable after being stripped off the hybridized target DNA from the grating surface, demonstrating a function of multiple usability.
Chen
Xianfeng
X
School of Engineering and Applied Science, Aston University, Birmingham, B4 7ET, UK. chenx2@aston.ac.uk
Zhang
Lin
L
Zhou
Kaiming
K
Davies
Edward
E
Sugden
Kate
K
Bennion
Ian
I
Hughes
Marcus
M
Hine
Anna
A
D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis*; D004867:Equipment Design; D004871:Erbium / Q000737:chemistry; D007368:Interferometry / Q000379:methods; D007834:Lasers; D008027:Light; D008962:Models, Theoretical; D009991:Oscillometry; D012666:Semiconductors; D013997:Time Factors; D015018:Ytterbium / Q000737:chemistry
D004871:Erbium; D004247:DNA; D015018:Ytterbium