Efficient transduction of spiral ganglion neurons in vitro by baculovirus vectors.
Degeneration of peripheral auditory neurons constitutes one of the main causes of sensorineural hearing loss. Gene delivery to the inner ear is central to the development of gene therapy for hearing impairment. Thus we investigated the effectiveness of baculovirus-derived vectors to transduce spiral ganglion neurons. We found that baculovirus could efficiently transduce spiral ganglion neurons in vitro and that the highest transduced cell rate could be over 75%. The level of transgene expression exhibited viral dose dependence and was enhanced by the addition of butyrate. Thus, baculovirus is a novel and promising tool for gene transfer into the cochlear nervous system, both in studies of the function of foreign genes and in the development of gene-therapy strategies.
Wang
Jun
J
Department of Otolaryngology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Li
Biao
B
Cai
Changping
C
Zhang
Yifan
Y
Wang
Shili
S
Hu
Shengping
S
Tian
Xianglong
X
Zhang
Min
M
D000818:Animals; D000831:Animals, Newborn; D016367:Baculoviridae / Q000502:physiology*; D005822:Genetic Vectors / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D066298:In Vitro Techniques; D009474:Neurons / Q000502:physiology* / Q000821:virology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013136:Spiral Ganglion / Q000166:cytology*; D014161:Transduction, Genetic / Q000379:methods*
D049452:Green Fluorescent Proteins