Terrabacter aerolatus sp. nov., isolated from an air sample.
A Gram-positive, strictly aerobic, motile, rod- or coccoid-shaped bacterium, strain 5516J-36(T), was isolated from an air sample from Jeju region, Korea, and its taxonomic position was investigated by a polyphasic approach. The organism grew optimally at 30 degrees C and pH 7.0-8.0. Comparative 16S rRNA gene sequencing studies demonstrated that this strain was highly related phylogenetically to Terrabacter terrae PPLB(T) and Terrabacter tumescens DSM 20308(T), showing 98.9 % sequence similarity to both strains. However, the DNA-DNA reassociation values between 5516J-36(T) and the type strains of Terrabacter terrae and Terrabacter tumescens were low (51 and 48 %, respectively). The peptidoglycan type was A3gamma, the predominant menaquinone was MK-8(H(4)), the polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and an unidentified phosphoglycolipid and the whole-cell sugars were glucose, ribose, rhamnose, xylose and galactose. Mycolic acids were absent. The major fatty acids (>5 % of total fatty acids) were iso-C(15 : 0), iso-C(16 : 0), iso-C(14 : 0), iso-C(17 : 0) and anteiso-C(15 : 0). The DNA G+C content was 71.7 mol%. On the basis of the above data, it is proposed that strain 5516J-36(T) represents a novel species, Terrabacter aerolatus sp. nov. The type strain of Terrabacter aerolatus is 5516J-36(T) (=KACC 20556(T) =DSM 18562(T)).
Weon
Hang-Yeon
HY
Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Schumann
Peter
P
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7b, 38124 Braunschweig, Germany.
Kroppenstedt
Reiner M
RM
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7b, 38124 Braunschweig, Germany.
Kim
Byung-Yong
BY
KACC - Korean Agricultural Culture Collection, Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Song
Jaekyeong
J
KACC - Korean Agricultural Culture Collection, Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Kwon
Soon-Wo
SW
KACC - Korean Agricultural Culture Collection, Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Go
Seung-Joo
SJ
KACC - Korean Agricultural Culture Collection, Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Stackebrandt
Erko
E
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7b, 38124 Braunschweig, Germany.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000332:Aerobiosis / Q000502:physiology; D000391:Air Microbiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008124:Locomotion / Q000502:physiology; D008969:Molecular Sequence Data; D009171:Mycolic Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis
D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009171:Mycolic Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2