title: [Effect of EphA2 protein on the expression of VEGF and MMP9 proteins in HCT116 cells].
abstract: OBJECTIVE
  To determine the effect of EphA2 protein on the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase 9 (MMP9) proteins in HCT116 cells.
  
  
  METHODS
  High expression of EphA2 protein in HCT116 cells was confirmed by Western blot. HCT116 cells were transfected with EphA2 antisense oligonucleotide. The expression of the transfection efficiency was analyzed by Western blot. VEGF proteins in the cell supernatants were detected by enzyme linked immunosorbent assay(ELISA), and the expressions of MMP9 in cell supernatants were examined by gelatin zymography.
  
  
  RESULTS
  EphA2 antisense oligonucleotide suppressed the expression of VEGF and MMP9 proteins in HCT116 cells.
  
  
  CONCLUSIONS
  EphA2 could decrease the invasion and metastasis of HCT116 cells by suppressing the expression of VEGF and MMP9.
authors.affiliation: Department of Pathology, College of Basic Medicine, Central South University, Changsha 410078, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
authors.forename: Jing-He
authors.initials: JH
authors.lastname: Li
authors.forename: Geng-Qiu
authors.initials: GQ
authors.lastname: Luo
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
mesh_terms: D045325:HCT116 Cells; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D016376:Oligonucleotides, Antisense; D036104:Receptor, EphA2 / Q000235:genetics / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D016376:Oligonucleotides, Antisense; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D036104:Receptor, EphA2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9