Delivery of macromolecules to plant parasitic nematodes using a tobacco rattle virus vector.
Plant parasitic nematodes cause significant damage to crops on a worldwide scale. These nematodes are often soil dwelling but rely on plants for food and to sustain them during reproduction. Complex interactions occur between plants and nematodes during the nematode life cycle with plant roots developing specialized feeding structures through which nematodes withdraw nutrients. Here we describe a novel method for delivering macromolecules to feeding nematodes using a virus-based vector [tobacco rattle virus (TRV)]. We show that the parasitic nematode Heterodera schachtii will ingest fluorescent proteins transiently expressed in plant roots infected with a TRV construct carrying the appropriate protein sequence. A prerequisite for this delivery is the presence of replicating virus in root tips prior to the formation of nematode-induced syncytia. We show also that TRV vectors expressing nematode gene sequences can be used to induce RNAi in the feeding nematodes.
Valentine
Tracy A
TA
Scottish Crop Research Institute, Invergowrie, Dundee, Scotland DD2 5DA, UK. tvalen@scri.sari.ac.uk
Randall
Eva
E
Wypijewski
Krzysztof
K
Chapman
Sean
S
Jones
John
J
Oparka
Karl J
KJ
D000818:Animals; D017360:Arabidopsis / Q000821:virology; D005247:Feeding Behavior; D015870:Gene Expression; D015726:Giant Cells; D010455:Peptides / Q000378:metabolism*; D010572:Pest Control, Biological; D010942:Plant Viruses / Q000378:metabolism*; D034622:RNA Interference; D012330:RNA, Double-Stranded; D014026:Nicotiana / Q000821:virology; D014431:Tylenchoidea
D010455:Peptides; D012330:RNA, Double-Stranded