Immobilization of Candida rugosa lipase on poly(allyl glycidyl ether-co-ethylene glycol dimethacrylate) macroporous polymer particles.
Macroporous polymer particles containing surface epoxy groups were synthesized for immobilization of Candida rugosa lipase (CRL). The effect of incorporation of two different sets of monomers [allyl glycidyl ether (AGE) and glycidyl methacrylate (GMA)] and the effect of crosslinking density on immobilization of lipase were studied. AGE-co-EGDM polymers gave higher binding and expression of lipase than GMA-co-EGDM polymers. Optimization of immobilization parameters was done with respect to immobilization time and enzyme loading. Amongst AGE-co-EGDM polymer series, AGE-150 polymer found to give maximum lipase activity yield and therefore evaluated for temperature, pH and storage stability. Under optimum conditions, AGE-150 polymer gave 78.40% of activity yield. Immobilized lipase on AGE-150 showed a broader pH, higher temperature and excellent storage stability.
Vaidya
Bhalchandra K
BK
Chemical Engineering and Process Development Division, National Chemical Laboratory, Pune 411 008, India.
Ingavle
Ganesh C
GC
Ponrathnam
S
S
Kulkarni
B D
BD
Nene
Sanjay N
SN
D002175:Candida / Q000187:drug effects / Q000201:enzymology*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism*; D008689:Methacrylates / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism*; D016062:Porosity / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D004852:Epoxy Compounds; D008689:Methacrylates; D011108:Polymers; C004919:ethylene dimethacrylate; D008049:Lipase; C054361:allyl glycidyl ether