Modeling HIV quasispecies evolutionary dynamics.
BACKGROUND
During the HIV infection several quasispecies of the virus arise, which are able to use different coreceptors, in particular the CCR5 and CXCR4 coreceptors (R5 and X4 phenotypes, respectively). The switch in coreceptor usage has been correlated with a faster progression of the disease to the AIDS phase. As several pharmaceutical companies are starting large phase III trials for R5 and X4 drugs, models are needed to predict the co-evolutionary and competitive dynamics of virus strains.


RESULTS
We present a model of HIV early infection which describes the dynamics of R5 quasispecies and a model of HIV late infection which describes the R5 to X4 switch. We report the following findings: after superinfection (multiple infections at different times) or coinfection (simultaneous infection by different strains), quasispecies dynamics has time scales of several months and becomes even slower at low number of CD4+ T cells. Phylogenetic inference of chemokine receptors suggests that viral mutational pathway may generate a large variety of R5 variants able to interact with chemokine receptors different from CXCR4. The decrease of CD4+ T cells, during AIDS late stage, can be described taking into account the X4-related Tumor Necrosis Factor dynamics.


CONCLUSIONS
The results of this study bridge the gap between the within-patient and the inter-patients (i.e. world-wide) evolutionary processes during HIV infection and may represent a framework relevant for modeling vaccination and therapy.
Sguanci
Luca
L
CSDC, Center for the Study of Complex Systems, Firenze, Italy. luca.sguanci@unifi.it
Bagnoli
Franco
F
Liò
Pietro
P
D000940:Antigenic Variation / Q000235:genetics; D019143:Evolution, Molecular; D015488:HIV Antigens / Q000235:genetics / Q000276:immunology; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000276:immunology; D006801:Humans; D016013:Likelihood Functions; D008954:Models, Biological; D009154:Mutation; D010641:Phenotype; D010802:Phylogeny; D019713:Receptors, CCR5 / Q000235:genetics* / Q000276:immunology; D019718:Receptors, CXCR4 / Q000235:genetics* / Q000276:immunology; D015163:Superinfection / Q000276:immunology / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology; D013997:Time Factors
D015488:HIV Antigens; D019713:Receptors, CCR5; D019718:Receptors, CXCR4