title: Purification, crystallization and preliminary X-ray characterization of a human mitochondrial phenylalanyl-tRNA synthetase.
abstract: Human monomeric mitochondrial phenylalanyl-tRNA synthetase (mitPheRS) is an enzyme that catalyzes the charging of tRNA with the cognate amino acid phenylalanine. Human mitPheRS is a chimera of the bacterial alpha-subunit of PheRS and the B8 domain of its beta-subunit. Together, the alpha-subunit and the 'RNP-domain' (B8 domain) at the C-terminus form the minimal structural set to construct an enzyme with phenylalanylation activity. The recombinant human mitPheRS was purified to homogeneity and crystallized in complex with phenylalanine and ATP. The crystals diffracted to 2.2 A resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 55, b = 90, c = 96 A.
authors.affiliation: Department of Structural Biology, Weizmann Institute of Science, 76100 Rehovot, Israel.
authors.forename: Inna
authors.initials: I
authors.lastname: Levin
authors.forename: Naama
authors.initials: N
authors.lastname: Kessler
authors.forename: Nina
authors.initials: N
authors.lastname: Moor
authors.forename: Liron
authors.initials: L
authors.lastname: Klipcan
authors.forename: Emine
authors.initials: E
authors.lastname: Koc
authors.forename: Paul
authors.initials: P
authors.lastname: Templeton
authors.forename: Linda
authors.initials: L
authors.lastname: Spremulli
authors.forename: Mark
authors.initials: M
authors.lastname: Safro
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008928:Mitochondria / Q000201:enzymology*; D010649:Phenylalanine / Q000378:metabolism; D010652:Phenylalanine-tRNA Ligase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D010649:Phenylalanine; D000255:Adenosine Triphosphate; D010652:Phenylalanine-tRNA Ligase