Lactadherin mediates sickle cell adhesion to vascular endothelial cells in flowing blood.
Increased exposure of sickle red blood cells to phosphatidylserine promotes its adhesion to the endothelium. A monoclonal antibody to lactadherin, a phosphatidylserine binding protein, inhibits sickle cell adhesion to histamine-stimulated endothelial cells in flowing blood. Added lactadherin enhances the adhesion via the integrin alphaVbeta3. These results indicate that lactadherin can mediate phosphatidylserine-expressing sickle cell adhesion to the endothelium.
Guchhait
Prasenjit
P
Dasgupta
Swapan K
SK
Le
Anhquyen
A
Yellapragada
Sarvari
S
López
José A
JA
Thiagarajan
Perumal
P
D000755:Anemia, Sickle Cell / Q000097:blood*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002417:Cattle; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism; D004913:Erythrocytes, Abnormal / Q000378:metabolism*; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D051379:Mice; D010587:Phagocytosis / Q000502:physiology*; D010718:Phosphatidylserines / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
D000911:Antibodies, Monoclonal; D039302:Integrin alphaVbeta3; D010718:Phosphatidylserines