title: Crystallization and preliminary crystallographic studies of the metalloglycoprotein esterase A4 using a baculovirus expression system.
abstract: Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori. The gene for this metalloglycoprotein was cloned from B. mori eggs and expressed using a baculovirus expression system in silkworm pupae. Crystals of the purified protein have been grown that diffract to beyond 2.1 A resolution at 100 K using synchrotron radiation. The protein crystals belong to space group P2(1), with unit-cell parameters a = 47.1, b = 73.9, c = 47.4 A, beta = 104.1 degrees. With one dimer per asymmetric unit, the crystal volume per unit protein weight (V(M)) is 2.3 A3 Da(-1) and the solvent content is 47%.
authors.affiliation: Protein Design Laboratory, Yokohama City University, 1-7-29 Suehiro, Tsurumi, Yokohama 230-0045, Japan.
authors.forename: Toshiki
authors.initials: T
authors.lastname: Hiraki
authors.forename: Naoya
authors.initials: N
authors.lastname: Shibayama
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Yoon
authors.forename: Kyung-Mook
authors.initials: KM
authors.lastname: Yun
authors.forename: Toshiro
authors.initials: T
authors.lastname: Hamamoto
authors.forename: Jeremy R H
authors.initials: JR
authors.lastname: Tame
authors.forename: Sam-Yong
authors.initials: SY
authors.lastname: Park
mesh_terms: D000818:Animals; D016367:Baculoviridae; D001483:Base Sequence; D012831:Bombyx / Q000201:enzymology; D043182:Carboxylesterase / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification; D006031:Glycosylation; D008667:Metalloproteins / Q000737:chemistry / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010063:Ovum / Q000201:enzymology; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D017931:DNA Primers; D006023:Glycoproteins; D008667:Metalloproteins; D011994:Recombinant Proteins; D043182:Carboxylesterase