title: Tumour necrosis factor a -308 promoter polymorphism in patients with rheumatoid arthritis.
abstract: There are controversial reports that TNF-a promoter polymorphism may be an independent marker of susceptibility to rheumatoid arthritis (RA). We used Polymerase chain reaction amplification and Restriction fragment length polymorphism for analysis of the polymorphism at position -308 in promoter of TNF-alpha gene in 34 patients with RA and 30 healthy individuals. Distribution of TNF-genotypes in RA patients did not differ from that in controls. Moreover, there was apparent association between the -308 TNF-alpha polymorphism and erosions in hand x-Ray was found (P value = 0.043). We suggest that TNF-alpha -308 promoter polymorphism is not a genetic risk factor for RA susceptibility but may be associated with radiographic damage in rheumatoid patients.
authors.affiliation: Department of Internal Medicine, Division of Rheumatology, Ghaem Hospital, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran. rezaieyazdi-z@mums.ac.ir
authors.forename: Zahra
authors.initials: Z
authors.lastname: Rezaieyazdi
authors.forename: Jalil Tavakol
authors.initials: JT
authors.lastname: Afshari
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Sandooghi
authors.forename: Farnaz
authors.initials: F
authors.lastname: Mohajer
mesh_terms: D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000235:genetics*; D016022:Case-Control Studies; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers / Q000737:chemistry; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006225:Hand / Q000000981:diagnostic imaging / Q000473:pathology; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic; D011859:Radiography; D012307:Risk Factors; D012720:Severity of Illness Index; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D014965:X-Rays
chemical_list: D017931:DNA Primers; D014409:Tumor Necrosis Factor-alpha; D004247:DNA