Antimicrobial activity of the methanolic extract, fractions and compounds from the stem bark of Irvingia gabonensis (Ixonanthaceae).
The antimicrobial activity of the methanolic extract from the stem bark of Irvingia gabonensis (IGM), fractions and compounds isolated from IGM [3-friedelanone (1), betulinic acid (2), oleanolic acid (3), 3,3',4'-tri-O-methylellagic acid (4), 3,4-di-O-methylellagic acid (5) and hardwickiic acid (6)] was evaluated against Gram-positive bacteria (6 species), Gram-negative bacteria (13 species) and three Candida species using dilution methods for the determination of the minimal inhibition concentration (MIC) and the minimal microbicidal concentration (MMC). From the obtained results, IGM prevented the growth of all the species of microorganisms tested at a concentration limit of 312.50 microg/ml. Compounds 4-6 also inhibited the growth of all the tested microbial species while compounds 1-3 showed selective activities. The lowest MIC values (78.12 microg/ml) were obtained with IGM on 13 of the 22 microorganisms tested. The corresponding value of 1.22 microg/ml (4.26 microM) for compounds was recorded with compound 6 on Neisseria gonorrhoeae. The obtained results confirmed the use of Irvingia gabonensis in the treatment of bacterial and fungal infections.
Kuete
Victor
V
Department of Biochemistry, Faculty of Science, University of Yaoundé I, BP 812, Yaoundé, Cameroon. kuetevictor@yahoo.fr
Wabo
Ghislain Fotso
GF
Ngameni
Bathélémy
B
Mbaveng
Armelle Tsafack
AT
Metuno
Robert
R
Etoa
François-Xavier
FX
Ngadjui
Bonaventure Tchaleu
BT
Beng
Véronique Penlap
VP
Meyer
J J Marion
JJ
Lall
Namrita
N
D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002163:Cameroon; D002175:Candida / Q000187:drug effects; D002482:Cellulose / Q000737:chemistry*; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology; D004610:Ellagic Acid / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009828:Oleanolic Acid / Q000302:isolation & purification / Q000494:pharmacology; D053978:Pentacyclic Triterpenes; D024301:Plant Bark; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology; D000094062:Betulinic Acid
C526107:3,4-di-O-methylellagic acid; C526105:3-friedelanone; D000890:Anti-Infective Agents; D004224:Diterpenes; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts; D014315:Triterpenes; C051375:dikanut; C052418:3,7,8-tri-O-methylellagic acid; D004610:Ellagic Acid; C074343:hardwickic acid; D009828:Oleanolic Acid; D002482:Cellulose; D000094062:Betulinic Acid