Modeling of product removal during enzymatic conversions by using affinity molecules.
The feasibility of using magnetic particles for in-line product isolation during enzymatic conversion was studied. A comparison was made between a process based on magnetic particles and a conventional adsorption column. The enzymatic reaction was described by two consecutive first-order reactions (synthesis and subsequent hydrolysis), while the adsorption of substrate and product was described by multicomponent Langmuir isotherms. The yield as well as synthesis/hydrolysis ratio were calculated for various system characteristics. The results show that magnetic particles are very effective when the affinity with the particles is specific and for enzymatic conversions involving low ratios of the rate of synthesis versus the rate of hydrolysis. For slow conversions and for low specific affinity molecules column separations are more appropriate.
Halsema
Daniël G R
DG
Food and Bioprocess Engineering Group, Wageningen University and Research Center, PO Box 8129, 6700 EV, Wageningen, The Netherlands. Daniel.halsema@wur.nl
Janssen
Anja E M
AE
Boom
Remko M
RM
D000327:Adsorption; D019149:Bioreactors; D006868:Hydrolysis; D007700:Kinetics; D008280:Magnetics; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000302:isolation & purification; D001617:beta-Glucosidase / Q000378:metabolism