title: Comparison of growth and susceptibility to sodium hypochlorite of mono- and dual-species biofilms of Fusobacterium nucleatum and Peptostreptococcus (micromonas) micros.
abstract: This study compared growth and susceptibility to different concentrations of sodium hypochlorite (NaOCl) of mono- and dual-species biofilms of Fusobacterium nucleatum or Peptostreptococcus micros in vitro at 24 hours or 96 hours. A Mann-Whitney U test revealed that although at 24 hours dual-species biofilms had similar viable counts to those of monospecies biofilms (p > 0.001), they were more resistant to NaOCl (p < 0.001). At 96 hours, both microorganisms had higher viable counts and were more resistant to NaOCl in dual-species biofilms than in monospecies biofilms of the same microorganism (p < 0.001). As the age of the biofilms increased, so did their resistance to NaOCl. Mixed-species biofilms of F. nucleatum and P. micros showed a time-dependent synergy in growth and resistance to NaOCl.
authors.affiliation: Department of Cariology Endodontology Pedodontology, Academic Center for Dentistry Amsterdam, Amsterdam, The Netherlands. r.ozok@acta.nl
authors.forename: Ahmet Rifat
authors.initials: AR
authors.lastname: Ozok
authors.forename: Min-Kai
authors.initials: MK
authors.lastname: Wu
authors.forename: Suzanne Bernardina Ida
authors.initials: SB
authors.lastname: Luppens
authors.forename: Paul Rudolf
authors.initials: PR
authors.lastname: Wesselink
mesh_terms: D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D015169:Colony Count, Microbial; D019551:Dental Disinfectants / Q000627:therapeutic use*; D003786:Dental Pulp Cavity / Q000382:microbiology; D016967:Fusobacterium nucleatum / Q000187:drug effects* / Q000502:physiology; D010462:Peptostreptococcus / Q000187:drug effects* / Q000502:physiology; D012973:Sodium Hypochlorite / Q000627:therapeutic use*; D013997:Time Factors