Expression of placental glutathione S-transferase in rat tongue mucosa exposed to cigarette smoke.
Glutatione S-transferases (GSTs) are a family of enzymes involved in detoxification of xenobiotics. Placental GST, known as GST-P, has been detected in tissues following exposure to carcinogenic agents being regarded a reliable biomarker of exposure and susceptibility in early phases of carcinogenesis. The aim of this study was to investigate the expressivity of GST-P positive foci in the rat tongue mucosa exposed to cigarette smoke by means of immunohistochemistry. A total of twelve male Wistar rats were distributed into two groups: negative control and experimental group exposed to cigarette smoke during 75 days. After experimental period, no histopathological changes in the tongue mucosa were evidenced in the negative control and the experimental group. However, a total of five GST-P positive foci were detected in two out of six animals exposed to cigarrette smoke. None control animals were noticed GST-P positive foci. These data indicate that expression of GST-P may reflect the carcinogenic effect of cigarette smoke as well as the genetic susceptibility of animals in relation to continuous carcinogens exposure.
Ribeiro
Daniel A
DA
Department of Health Sciences, Federal University of Sao Paulo, UNIFESP, Av. Ana Costa 95, Santos, SP, 11060-001, Brazil. daribeiro@unifesp.br
Assis
Gerson F
GF
D000818:Animals; D051549:Glutathione S-Transferase pi / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009061:Mouth Mucosa / Q000166:cytology / Q000201:enzymology*; D010920:Placenta / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D012907:Smoking; D014026:Nicotiana; D014059:Tongue / Q000166:cytology / Q000201:enzymology*