Expression of bone extracellular matrix proteins on osteoblast cells in the presence of mineral trioxide.
The biocompatibility of periapical tissue with mineral trioxide aggregate (MTA) affects its ability to repair and regenerate itself. Here we report the cytotoxicity of MTA and how it affects the expression of bone extracellular matrix protein in MC3T3-E1 osteoblast cells. We quantified the cytotoxicity of MTA, amalgam, and Dycal (Dentsply/Caulk, Milford, DE) on MC3T3-E1 cells by measuring the ability of cells to cleave a tetrazolium salt to produce formazan dye during a period of 24, 48, or 96 hours. We used reverse-transcriptase polymerase chain reaction with primer sets for type I collagen, osteocalcin, and bone sialoprotein to measure the gene-expression response of MC3T3-E1 cells treated with MTA. MTA, amalgam, and Dycal were less toxic after 48 hours. MC3T3-E1 cell growth with MTA and Dycal was greater than nonstimulated controls. MTA caused an upregulation of type I collagen and osteocalcin messenger RNA expression after 24 hours. These results showed that, in the presence of MTA, cells grow faster and produce more mineralized matrix gene expression in osteoblasts.
Tani-Ishii
Nobuyuki
N
Kanagawa Dental College, Yokosuka, Kanagawa, Japan.
Hamada
Nobushiro
N
Watanabe
Kiyoko
K
Tujimoto
Yasuhisa
Y
Teranaka
Toshio
T
Umemoto
Toshio
T
D017607:Aluminum Compounds / Q000633:toxicity*; D001672:Biocompatible Materials / Q000633:toxicity*; D017610:Calcium Compounds / Q000633:toxicity*; D002126:Calcium Hydroxide / Q000633:toxicity; D024042:Collagen Type I / Q000032:analysis; D003723:Dental Amalgam / Q000633:toxicity; D003764:Dental Materials / Q000633:toxicity; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins / Q000187:drug effects*; D058574:Integrin-Binding Sialoprotein; D008903:Minerals / Q000633:toxicity; D010006:Osteoblasts / Q000187:drug effects*; D015675:Osteocalcin / Q000032:analysis; D010087:Oxides / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000032:analysis; D017640:Silicates / Q000633:toxicity*; D013997:Time Factors