Expression profile of class I histone deacetylases in human cancer tissues.
Histone deacetylase (HDAC) activity is one of the widely used and well-established mechanisms for regulation of various genes in cancer. To identify which subtype of class I HDACs are overexpressed in cancers, we analyzed the expression of class I HDAC isotypes composed of HDAC1, 2, 3 and 8 in several cell lines and human cancer tissues, including cancer of the stomach, esophagus, colon, prostate, breast, ovary, lung, pancreas and thyroid. The results showed that >75% of human cancer tissues and their corresponding non-cancerous epithelium showed high expression of these class I HDACs. However, the immunoreactivity of HDAC8 in both prostatic cancer tissue and non-cancerous prostate glands was lower than that in other cancer tissues. Furthermore, 5-40% of cancer tissues overexpressed class I HDACs, when compared with normal epithelium. The results suggest the potential usefulness of HDAC inhibitors for the treatment of a wide variety of human cancers.
Nakagawa
Masamune
M
Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Oda
Yoshinao
Y
Eguchi
Takashi
T
Aishima
Shin-Ichi
S
Yao
Takashi
T
Hosoi
Fumihito
F
Basaki
Yuji
Y
Ono
Mayumi
M
Kuwano
Michihiko
M
Tanaka
Masao
M
Tsuneyoshi
Masazumi
M
D015153:Blotting, Western; D004847:Epithelial Cells / Q000201:enzymology / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D056284:Histone Deacetylase 1; D056464:Histone Deacetylase 2; D006655:Histone Deacetylases / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D009369:Neoplasms / Q000201:enzymology* / Q000473:pathology; D012097:Repressor Proteins / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000099186:Histone Deacetylase 3