title: CAP2, cyclase-associated protein 2, is a dual compartment protein.
abstract: Cyclase-associated proteins (CAPs) are evolutionarily conserved proteins with roles in regulating the actin cytoskeleton and in signal transduction. Mammals have two CAP genes encoding the related CAP1 and CAP2. We studied the distribution and subcellular localization of CAP1 and CAP2 using specific antibodies. CAP1 shows a broad tissue distribution, whereas CAP2 is significantly expressed only in brain, heart and skeletal muscle, and skin. CAP2 is found in the nucleus in undifferentiated myoblasts and at the M-line of differentiated myotubes. In PAM212, a mouse keratinocyte cell line, CAP2 is enriched in the nucleus, and sparse in the cytosol. By contrast, CAP1 localizes to the cytoplasm in PAM212 cells. In human skin, CAP2 is present in all living layers of the epidermis localizing to the nuclei and the cell periphery. In in vitro studies, a C-terminal fragment of CAP2 interacts with actin, indicating that CAP2 has the capacity to bind to actin.
authors.affiliation: Institute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 52, 50931, Köln, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Peche
authors.forename: S
authors.initials: S
authors.lastname: Shekar
authors.forename: M
authors.initials: M
authors.lastname: Leichter
authors.forename: H
authors.initials: H
authors.lastname: Korte
authors.forename: R
authors.initials: R
authors.lastname: Schröder
authors.forename: M
authors.initials: M
authors.lastname: Schleicher
authors.forename: T A
authors.initials: TA
authors.lastname: Holak
authors.forename: C S
authors.initials: CS
authors.lastname: Clemen
authors.forename: B
authors.initials: B
authors.lastname: Ramanath-Y
authors.forename: G
authors.initials: G
authors.lastname: Pfitzer
authors.forename: I
authors.initials: I
authors.lastname: Karakesisoglou
authors.forename: A A
authors.initials: AA
authors.lastname: Noegel
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins / Q000032:analysis* / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry*; D003598:Cytoskeletal Proteins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000737:chemistry; D014018:Tissue Distribution; D048868:Adaptor Proteins, Signal Transducing