Review of centrifugal liquid-liquid chromatography using aqueous two-phase solvent systems: its scale-up and prospects for the future production of high-value biologicals.
Future challenges in the field of bioprocessing include developing new downstream processes for the purification and manufacture of protein-based medicines to relieve the predicted bottleneck that may occur as a result of increasingly high titers obtained from fermentation processes. This review considers recent developments in centrifugal liquid-liquid partition chromatography using aqueous two-phase solvent systems, a gentle host medium for biologicals, and the prospect for scale-up and eventual manufacture of high-value pharmaceutical products.
Sutherland
Ian A
IA
Brunel Institute for Bioengineering, Brunel University, Uxbridge, UB8 3PH, UK. ian.sutherland@brunel.ac.uk
D001702:Biopharmaceutics / Q000295:instrumentation / Q000639:trends; D002498:Centrifugation; D002853:Chromatography, Liquid / Q000379:methods*; D015195:Drug Design; D044367:Phase Transition; D012997:Solvents / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000639:trends