[Studies on HPLC-fingerprint of Rhizoma Fagopyri Dibotoryis].
OBJECTIVE
To establish an HPLC fingerprint of Rhizoma fagopyri dibotoryis.


METHODS
The HPLC-electrochemical detection assay was used to establish the fingerprint of Rhizoma fagopyri dibotoryis. The sample was performed on a column of Diamonsil C18 (4.6 mm x 250 mm, 5 microm) which was eluted with methanol- 0.1 mol x L(-1) phosphate buffer (pH 2.5), the flow rate was 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the work electrode was glassy carbon, the counter electrode was Pt platmun.


RESULTS
The HPLC fingerprint profiles of 6 Rhizoma fagopyri dibotoryis contains 6 common chromatographic peaks, and gallic acid, protocatechuic acid, protocatechuic aldehyde and ( - ) -epicatechin were tested the samples. The contents of protocatechuic acid and protocatechuic aldehyde were from 0.004% to 0.05% and from 0.003% to 0.015%, respectively.


CONCLUSIONS
The method can be used to control the quality of Rhizoma fagopyri dibotoryis.
Yang
Bin
B
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China. ybinmm@hotmail.com
Xin
Min-tong
MT
Lu
Dong-mei
DM
Qu
Qiao-ling
QL
Li
Hua
H
Huang
Lu-qi
LQ
D001547:Benzaldehydes / Q000032:analysis; D002392:Catechin / Q000032:analysis; D002396:Catechols / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000592:standards; D004563:Electrochemistry; D019612:Fagopyrum / Q000737:chemistry*; D005707:Gallic Acid / Q000032:analysis; D062385:Hydroxybenzoates / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry*