title: Survival in the presence of antifungals: genome-wide expression profiling of Aspergillus niger in response to sublethal concentrations of caspofungin and fenpropimorph.
abstract: How yeast cells respond to cell wall stress is relatively well understood; however, how filamentous fungi cope with cell wall damage is largely unexplored. Here we report the first transcriptome analysis of Aspergillus niger exposed to the antifungal compounds caspofungin, an inhibitor of beta-1,3-glucan synthesis, and fenpropimorph, which inhibits ergosterol synthesis. The presence of sublethal drug concentrations allowed A. niger to adapt to the stress conditions and to continue growth by the establishment of new polarity axes and formation of new germ tubes. By comparing the expression profile between caspofungin-exposed and nonexposed A. niger germlings, we identified a total of 172 responsive genes out of 14,509 open reading frames present on the Affymetrix microarray chips. Among 165 up-regulated genes, mainly genes predicted to function in (i) cell wall assembly and remodeling, (ii) cytoskeletal organization, (iii) signaling, and (iv) oxidative stress response were affected. Fenpropimorph modulated expression of 43 genes, of which 41 showed enhanced expression. Here, genes predicted to function in (i) membrane reconstruction, (ii) lipid signaling, (iii) cell wall remodeling, and (iv) oxidative stress response were identified. Northern analyses of selected genes were used to confirm the microarray analyses. The results further show that expression of the agsA gene encoding an alpha-1,3-glucan synthase is up-regulated by both compounds. Using two PagsA-GFP reporter strains of A. niger and subjecting them to 16 different antifungal compounds, including caspofungin and fenpropimorph, we could show that agsA is specifically activated by compounds interfering directly or indirectly with cell wall biosynthesis.
authors.affiliation: Department of Microbiology and Genetics, Institute of Biotechnology, Berlin University of Technology, Gustav-Meyer-Allee 25, Berlin, Germany. v.meyer@lb.tu-berlin.de
authors.forename: Vera
authors.initials: V
authors.lastname: Meyer
authors.forename: Robbert A
authors.initials: RA
authors.lastname: Damveld
authors.forename: Mark
authors.initials: M
authors.lastname: Arentshorst
authors.forename: Ulf
authors.initials: U
authors.lastname: Stahl
authors.forename: Cees A M J J
authors.initials: CA
authors.lastname: van den Hondel
authors.forename: Arthur F J
authors.initials: AF
authors.lastname: Ram
mesh_terms: D000935:Antifungal Agents / Q000633:toxicity*; D001234:Aspergillus niger / Q000166:cytology / Q000187:drug effects* / Q000235:genetics*; D000077336:Caspofungin; D016764:Cell Polarity; D054714:Echinocandins / Q000633:toxicity*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D016681:Genome, Fungal / Q000187:drug effects*; D055666:Lipopeptides; D009025:Morpholines / Q000633:toxicity*; D014158:Transcription, Genetic / Q000235:genetics