[Change of microbial community in rhizoma sphere of cultivated Atractylodes lancea].
OBJECTIVE
To find the development rules of microbial community in rhizoma sphere of the cultivated Atractylodes lancea.


METHODS
Total bacteria, fungi and actinomyces were counted by CFU x g(-1) though dilution plate method. And genomic DNA of microbes were extracted and amplified by primers of E. coli's 27f and 1492r to get the 16S rDNA, then the restriction endonuclease Hinf was used to digest the 16S rDNA.


RESULTS
Total bacteria, fungi and actinomyces in 2-year old soil were lower than in 1-year old soil, they decreased 46. 14%, 49. 25%, 31.88% respectively and made the ratio of themselves changed. At the same time, all the 8 soil samples got fine 16S rDNA bands, which were about 1500 bp. And the main bands of most of the samples were found at 1000 bp, but the weak bands of each were different although most bands in the same year samples were more similar than in different year ones.


CONCLUSIONS
It is indicated that the change of soil microbial community may has some relation to the continous cropping barrier of A. lancea.
Guo
Lan-ping
LP
Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China.
Huang
Lu-qi
LQ
Jiang
You-xu
YX
Chen
Mei-lan
ML
Lv
Dong-mei
DM
Zeng
Yan
Y
D000190:Actinomyces / Q000235:genetics / Q000302:isolation & purification; D031174:Atractylodes / Q000254:growth & development*; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D015169:Colony Count, Microbial; D004275:DNA, Ribosomal / Q000235:genetics; D005658:Fungi / Q000235:genetics / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000254:growth & development*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D027343:Rhizome / Q000254:growth & development; D012988:Soil Microbiology