title: Antigen preparation for immunological studies in systemic autoimmune diseases.
abstract: Systemic autoimmune diseases are characterized by the presence in patient sera of high titers of autoantibodies directed against self-antigens. The characterization of specific autoantigen-autoantibody system is very important for research as well as for diagnostic tools and several techniques can be employed in such immunological studies. In this review an overview of the different biochemical methods of antigen preparation and the specific methodological applications is given, also describing potential critical aspects and benefits of the different protocols.
authors.affiliation: Division of Rheumatology, Department of Clinical and Experimental Medicine, University of Padova, Padova, Italy.
authors.forename: Sandra
authors.initials: S
authors.lastname: Zampieri
authors.forename: Anna
authors.initials: A
authors.lastname: Ghirardello
authors.forename: Katia
authors.initials: K
authors.lastname: Rossini
authors.forename: Luca
authors.initials: L
authors.lastname: Iaccarino
authors.forename: Nicola
authors.initials: N
authors.lastname: Bassi
authors.forename: Fabiola
authors.initials: F
authors.lastname: Atzeni
authors.forename: Piercarlo
authors.initials: P
authors.lastname: Sarzi-Puttini
authors.forename: Andrea
authors.initials: A
authors.lastname: Doria
mesh_terms: D000941:Antigens / Q000276:immunology* / Q000302:isolation & purification; D001323:Autoantibodies / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology* / Q000473:pathology; D006801:Humans; D008214:Lymphocytes / Q000276:immunology