Cadmium-Resistant Pseudomonas putida Synthesizes Novel Cadmium Proteins.
Three cysteine-rich proteins of molecular weight 4000 to 7000, containing 4 to 7 gram atoms of cadmium, zinc, and copper per mole were isolated from Pseudomonas putida growing in 3 mM cadmium. The three proteins were induced during different phases of growth, and the smallest (molecular weight 3600; 3 gram atoms of cadmium) was released into the medium when the cells lysed. The results of amino acid analyses and of ultraviolet, circular dichroism, electron paramagnetic resonance, and cadmium-113 nuclear magnetic resonance spectroscopy suggest a novel cadmium(II)-zinc(II)-copper(I) cluster structure for the major protein.
Higham
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DP
Sadler
P J
PJ
Scawen
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MD