title: Impaired plasmacytoid dendritic cell innate immune responses in patients with herpes virus-associated acute retinal necrosis.
abstract: Plasmacytoid dendritic cells (PDC), the main producers of type I IFNs in the blood, are important for the recognition and control of viral and bacterial infections. Because several viruses induce IFN-alpha production, severe courses of herpes virus infections in nonimmunocompromised patients may be related to numerical or functional PDC deficits. To evaluate this hypothesis, PBMC and PDC were repeatedly isolated from nine patients with acute retinal necrosis (ARN), caused by herpes simplex or varicella zoster virus. The patients experienced meningitis/encephalitis and frequent infections in childhood (n = 2), recurrent herpes virus infections at unusual localizations (n = 2), ocular surgery (n = 1), infections (n = 4), and stress around ARN (n = 6). The median percentage of isolated PDC was significantly lower in patients compared with 18 age-matched healthy controls (p < 0.001), confirmed by FACS analysis using peripheral blood, and was extremely low during acute disease. PDC counts dropped in five controls suffering from respiratory infections or diarrhea. IFN-alpha production in PDC and PBMC exposed to different stimuli was significantly lower in patients than in controls (p < 0.05). Anergy to these stimuli was observed on four occasions, in particular during acute disease. PDC of patients showed up-regulated IFN regulatory factor-7 mRNA levels and evidence of in vivo activation (CD80) and maturation (CD83) (p < 0.05). CD8+ cell responses were significantly lower in patients vs controls (p = 0.04). These data support a risk factor model in which numerical and functional deficits in PDC-mediated innate immune responses contribute to an impaired control of latent herpes virus infections and subsequent development of ARN.
authors.affiliation: Institute of Clinical and Molecular Virology, German National Reference Centre for Retroviruses, Erlangen, Germany.
authors.forename: Nicolai A
authors.initials: NA
authors.lastname: Kittan
authors.forename: Antonio
authors.initials: A
authors.lastname: Bergua
authors.forename: Sabrina
authors.initials: S
authors.lastname: Haupt
authors.forename: Norbert
authors.initials: N
authors.lastname: Donhauser
authors.forename: Philipp
authors.initials: P
authors.lastname: Schuster
authors.forename: Klaus
authors.initials: K
authors.lastname: Korn
authors.forename: Thomas
authors.initials: T
authors.lastname: Harrer
authors.forename: Barbara
authors.initials: B
authors.lastname: Schmidt
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001772:Blood Cell Count; D017635:Clonal Anergy; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D015536:Down-Regulation / Q000276:immunology; D006561:Herpes Simplex / Q000276:immunology* / Q000473:pathology; D014645:Herpesvirus 3, Human / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate; D050839:Interferon Regulatory Factor-7 / Q000096:biosynthesis / Q000235:genetics; D016898:Interferon-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism; D008875:Middle Aged; D015882:Retinal Necrosis Syndrome, Acute / Q000276:immunology* / Q000473:pathology / Q000821:virology*; D018139:Simplexvirus / Q000276:immunology*; D015854:Up-Regulation / Q000276:immunology