Transcription of productive and nonproductive VDJ-recombined alleles after IgH allelic exclusion.
The process of allelic exclusion ensures that each B cell expresses a B-cell receptor encoded by only one of its Ig heavy (IgH) and light (IgL) chain alleles. Although its precise mechanism is unknown, recruitment of the nonfunctional IgH allele to centromeric heterochromatin correlates with the establishment of allelic exclusion. Similarly, recruitment in activated splenic B cells correlates with cell division. In the latter, the recruited IgH allele was reported to be transcriptionally silent. However, it is not known whether monoallelic recruitment during establishment of allelic exclusion correlates with transcriptional silencing. To investigate this, we assessed the transcriptional status of both IgH alleles in single primary cells over the course of B-cell development, using RNA fluorescence in situ hybridization. Before allelic exclusion both alleles are transcribed. Thereafter, in pre-BII and subsequent developmental stages both functional and nonfunctional VDJ- and DJ-transcription is observed. Thus, after the establishment of IgH allelic exclusion, monoallelic recruitment to heterochromatin does not silence VDJ- or DJ-transcription, but serves another purpose.
Daly
Janssen
J
Laboratory of Lymphocyte Signalling and Development, The Babraham Institute, Cambridge, UK.
Licence
Steve
S
Nanou
Aikaterini
A
Morgan
Geoff
G
Mårtensson
Inga-Lill
IL
D000483:Alleles; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology*; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain / Q000502:physiology*; D020868:Gene Silencing / Q000502:physiology; D050438:Genes, Immunoglobulin Heavy Chain / Q000502:physiology*; D050439:Genes, Immunoglobulin Light Chain / Q000502:physiology; D006570:Heterochromatin / Q000276:immunology / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000276:immunology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D014158:Transcription, Genetic / Q000502:physiology*