Nullisomic tetrahymena: eliminating germinal chromosomes.
Germinal and somatic functions in Tetrahymena are performed separately by the micro- and macronuclei, respectively. Cells with haploid micronuclei were mated with diploids to yield monosomic progeny. These were induced to undergo a form of self-fertilization, generating cells lacking both copies of one or more of the five chromosomes in the micronucleus while still possessing a complete macronuclear genome.
Bruns
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PJ
Brussard
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