title: [Studies on HPLC-fingerprint of Rhizoma Fagopyri Dibotoryis].
abstract: OBJECTIVE
  To establish an HPLC fingerprint of Rhizoma fagopyri dibotoryis.
  
  
  METHODS
  The HPLC-electrochemical detection assay was used to establish the fingerprint of Rhizoma fagopyri dibotoryis. The sample was performed on a column of Diamonsil C18 (4.6 mm x 250 mm, 5 microm) which was eluted with methanol- 0.1 mol x L(-1) phosphate buffer (pH 2.5), the flow rate was 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the work electrode was glassy carbon, the counter electrode was Pt platmun.
  
  
  RESULTS
  The HPLC fingerprint profiles of 6 Rhizoma fagopyri dibotoryis contains 6 common chromatographic peaks, and gallic acid, protocatechuic acid, protocatechuic aldehyde and ( - ) -epicatechin were tested the samples. The contents of protocatechuic acid and protocatechuic aldehyde were from 0.004% to 0.05% and from 0.003% to 0.015%, respectively.
  
  
  CONCLUSIONS
  The method can be used to control the quality of Rhizoma fagopyri dibotoryis.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China. ybinmm@hotmail.com
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Min-tong
authors.initials: MT
authors.lastname: Xin
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Lu
authors.forename: Qiao-ling
authors.initials: QL
authors.lastname: Qu
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Lu-qi
authors.initials: LQ
authors.lastname: Huang
mesh_terms: D001547:Benzaldehydes / Q000032:analysis; D002392:Catechin / Q000032:analysis; D002396:Catechols / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000592:standards; D004563:Electrochemistry; D019612:Fagopyrum / Q000737:chemistry*; D005707:Gallic Acid / Q000032:analysis; D062385:Hydroxybenzoates / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry*