[Effect of concentration of catalpol and 5-hydroxymethyl-2-furaldehyde from processing of Rehmanniae Radix].
OBJECTIVE
To study on effect of concentration of catalpol and 5-hydroxy methyl-2-furaldehyde (5-HMF) from Rehmanniae Radix at various processing.


METHODS
The Rehmanniae Radix was dried and prepared from the steaming process with 10% ethanol, 50% ethanol at 90 degrees C and 100 degrees C each other. And the changes of catalpol and 5-HMF was determinated. The extraction of 5-HMF and catalpol was sonicated in 30% methanol for 2 h. The analysis of 5-HMF and catalpol was conducted by HPLC with reversed-phase C-18 column and detected under UV 284 nm, 204 nm. Elution was carried out at 1.0 mL min(-1) with 3% acetonitrile.


RESULTS
From this analysis, we found out that the content of catalpol was decreased with the number of processing times, and content of 5-HMF was increased with the number of processing times at various processing. The temperature and concentration of ethanol can effect on content of catalpol and 5-HMF at processing. The Cooked Rehmanniae Radix processed at 100 degrees C, 10% ethanol is best. And the content of 5-HMF processed for more than 7 times was accorded with standard of Korea phamcopoetia.


CONCLUSIONS
Analyze the effect of concentration of catalpol and 5-HMF from Rehmanniae Radix at various processing, and provide the foundation for further study.
Zhu
Mei-fen
MF
Department of Oriental Pharmaceutical Sciences, College of Phamacy, Kyung Hee University, Seoul 130701, Korea.
Liu
Xiang-qian
XQ
Ju-hee
Oh
O
Chang-soo
Yook
Y
Je-Hyun
Lee
L
D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis; D005960:Glucosides / Q000032:analysis*; D006358:Hot Temperature; D057888:Iridoid Glucosides; D039823:Iridoids / Q000032:analysis*; D035281:Plant Tubers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D032267:Rehmannia / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods