Protein expression profile of celiac disease patient with aberrant T cell by two-dimensional difference gel electrophoresis.
One complication of celiac disease (CD) is refractory CD. These patients frequently show aberrant intraepithelial T cell clones and an increasing risk of evolution into enteropathy-associated T cell lymphoma (EATL). There is debate in the literature whether these cases are actually a smoldering lymphoma from the outset. The mechanism inducing T cell proliferation and prognosis remains unknown. Recently, alemtuzumab has been proposed as a promising new approach to treat these patients. Only few single cases have been tested presently, nevertheless, in all of them a clinical improvement has been observed, while intraepithelial lymphocytes (IELs) effectively targeted by alemtuzumab are still a debated issue. Using 2D-DIGE, we found hyperexpressed proteins specifically associated with aberrant T cell in a patient with CD by comparing the protein expression with that of patients with CD and polyclonal T cell or with that of control subjects (patients with polyclonal T cell and no CD). Proteins with a higher expression in duodenal biopsy of the patient with aberrant T cell were identified as IgM, apolipoprotein C-III, and Charcot-Leyden crystal proteins. These preliminary data allow hypothesizing different clinical effects of alemtuzumab in patients with CD, since besides the probable effect of alemtuzumab on T cell, it could effect inflammatory-associated CD52(+) IgM(+)B cell and eosinophils cells, known to produce IgM and Charcot-Leyden crystal proteins, which we demonstrated to be altered in this patient. Results also emphasize the possible association of apolipoprotein with aberrant T cell proliferation.
De Re
Valli
V
Experimental and Clinical Farmacology Unit, DOMERT Department, Centro di Riferimento Oncologico, IRCCS, National Cancer Institute, Aviano (PN), Italy. vdere@cro.it
Simula
Maria Paola
MP
Caggiari
Laura
L
Ortz
Nicoletta
N
Spina
Michele
M
Da Ponte
Alessandro
A
De Appolonia
Leandro
L
Dolcetti
Riccardo
R
Canzonieri
Vincenzo
V
Cannizzaro
Renato
R
D000328:Adult; D000368:Aged; D053305:Apolipoprotein C-III / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002446:Celiac Disease / Q000145:classification / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology / Q000378:metabolism; D008245:Lysophospholipase / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics; D013601:T-Lymphocytes / Q000378:metabolism*