title: Sequence analysis of OmNramp alpha and quantitative expression of Nramp homologues in different trout strains after infection with Myxobolus cerebralis.
abstract: Salmonid whirling disease caused by the metazoan parasite Myxobolus cerebralis is an ongoing problem in wild and farmed rainbow trout Oncorhynchus mykiss populations. Rainbow trout from different strains vary in susceptibility to the parasite. Identification of underlying mechanisms could be a starting point for improved control of the disease. We conducted infection trials using 2 rainbow trout strains and brown trout Salmo trutta fario, a species not susceptible to the parasite, to investigate host immune response and resistance mechanisms. We compared expression levels of 2 natural resistance-associated macrophage proteins (Nramp alpha and beta) after infection with M. cerebralis. Total RNA was extracted from skin, muscle, kidney, head and spinal column, and gene expression was quantified by real-time PCR. Significant decreases in expression of both genes were observed at different time points in the infected susceptible rainbow trout compared to the non-infected group. Furthermore, the OmNramp alpha (O. mykiss natural resistance-associated macrophage protein alpha) sequences in 2 resistant and 1 non-resistant rainbow trout strain were analysed and compared for sequence aberrations.
authors.affiliation: Institute of Zoology, Fish Biology and Fish Diseases, University of Munich, Kaulbachstr. 37, 80539 Munich, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Rucker
authors.forename: Mansour
authors.initials: M
authors.lastname: El-Matbouli
mesh_terms: D000818:Animals; D027682:Cation Transport Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D056890:Eukaryota / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology* / Q000469:parasitology*; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000276:immunology*; D020641:Polymorphism, Single Nucleotide; D011529:Protozoan Infections, Animal / Q000276:immunology* / Q000469:parasitology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D000099173:Natural Resistance-associated Macrophage Protein 1