title: A novel role for the nuclear membrane protein emerin in association of the centrosome to the outer nuclear membrane.
abstract: The type II inner nuclear membrane protein emerin is a component of the LINC complex that connects the nuclear lamina to the actin cytoskeleton. In emerin-null or -deficient human dermal fibroblasts we find that the centrosome is detached from the nucleus. Moreover, following siRNA knockdown of emerin in wild-type fibroblasts, the centrosome also becomes detached from the nucleus. We show that emerin interacts with tubulin, and that nocadozole-treated wild-type cells phenocopy the detached centrosome characteristic of emerin-null/deficient cells. We also find that a significant fraction of emerin is located at the outer nuclear membrane and peripheral ER, where it interacts directly with the centrosome. Our data provide the first evidence in mammalian cells as to the nature of the linkage of the centrosome, and therefore the tubulin cytoskeleton, with the outer nuclear membrane.
authors.affiliation: School of Biological and Biomedical Sciences, The University of Durham, Durham, England, UK.
authors.forename: Georgia
authors.initials: G
authors.lastname: Salpingidou
authors.forename: Andrei
authors.initials: A
authors.lastname: Smertenko
authors.forename: Irena
authors.initials: I
authors.lastname: Hausmanowa-Petrucewicz
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Hussey
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Hutchison
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D018385:Centrosome / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009685:Nuclear Envelope / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D014404:Tubulin / Q000378:metabolism*