title: Gene sequence variations of the platelet P2Y12 receptor are associated with coronary artery disease.
abstract: BACKGROUND
  The platelet P2Y12 receptor plays a key role in platelet activation. The H2 haplotype of the P2Y12 receptor gene (P2RY12) has been found to be associated with maximal aggregation response to adenosine diphosphate (ADP) and with increased risk for peripheral arterial disease. No data are available on its association with coronary artery disease (CAD).
  
  
  METHODS
  The H2 haplotype of the P2RY12 was determined in 1378 unrelated patients of both sexes selected according to the presence of significant coronary artery disease (CAD group) or having normal coronary angiogram at cardiac catheterization (CAD-free group). Significant coronary artery disease was angiographically determined, and was defined as a greater than 50% visually estimated luminal diameter stenosis in at least one major epicardial coronary artery.
  
  
  RESULTS
  In the studied population 71.9% had CAD (n = 991) and 28.1% had normal coronary angiogram (n = 387). H2 haplotype carriers were more frequent in the CAD group (p = 0.03, OR = 1.36, 95%CI = 1.02-1.82). The H2 haplotype was significantly associated with CAD in non-smokers (p = 0.007, OR = 1.83 95%CI = 1.17-2.87), but not in smokers. The association remained significant after adjustment for other covariates (age, triglycerides, HDL, hypertension, diabetes) by multivariate logistic regression (p = 0.004, OR = 2.32 95%CI = 1.30-4.15).
  
  
  CONCLUSIONS
  Gene sequence variations of the P2Y12 receptor gene are associated with the presence of significant CAD, particularly in non-smoking individuals.
authors.affiliation: Department of Mother and Child and of Biology-Genetics, Section of Biology and Genetics, University of Verona, Verona, Italy. ugo.cav@fastwebnet.it
authors.forename: Ugo
authors.initials: U
authors.lastname: Cavallari
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Trabetti
authors.forename: Giovanni
authors.initials: G
authors.lastname: Malerba
authors.forename: Michele
authors.initials: M
authors.lastname: Biscuola
authors.forename: Domenico
authors.initials: D
authors.lastname: Girelli
authors.forename: Oliviero
authors.initials: O
authors.lastname: Olivieri
authors.forename: Nicola
authors.initials: N
authors.lastname: Martinelli
authors.forename: Dominick J
authors.initials: DJ
authors.lastname: Angiolillo
authors.forename: Roberto
authors.initials: R
authors.lastname: Corrocher
authors.forename: Pier Franco
authors.initials: PF
authors.lastname: Pignatti
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D018048:Receptors, Purinergic P2 / Q000235:genetics*; D058925:Receptors, Purinergic P2Y12