[Genotyping of gram-negative bacteria isolated from blood in sepsis in patients with hematological diseases].
OBJECTIVE
Genotyping of hospital strains of gram-negative bacteria isolated from blood of patients with hematological diseases in the presence of sepsis.


METHODS
Bacterial DNA were studied with pulse-electrophoresis with commercial kits Genepath Reagent Kits (BioRad, USA). Comparison and interpretation of the results were made with application of the computer analysis program Bionumerics, Applied Maths.


RESULTS
DNA of bacteria of 152 hospital strains was studied. In genotyping of 26 strains of P. aeruginosa 21 clones was isolated, 15 strains of Enterobacter spp.--12 clones, 31 strains of K. pneumoniae--27 clones, 80 strains of E.coli--79 clones. The greatest number of clones of identical bacteria (19%) was detected in the strains of P. aeruginosa, 17%--Enterobacter spp., 11%--K. pneumoniae, minimal (1%) in the strains of E. coli (p < 0.001). Incidence of isolation of clonal-relative strains among P. aeruginosa isolates was 35% (n = 9), Enterobacter spp.--33% (n = 5), K. pneumoniae--23% (n = 7), E. coli--2% (n = 2).


CONCLUSIONS
Polyclonality of the microorganisms was detected. Frequency of isolation of clones containing identical bacteria in the test bacteria was variable. In clinic there are endogenic and exogenic mechanisms of infection with prevalence of endogenic.
Kliasova
G A
GA
Brilliantova
A N
AN
Mironova
A V
AV
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006402:Hematologic Diseases / Q000382:microbiology*; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D018805:Sepsis / Q000150:complications* / Q000382:microbiology