The effect of topical mitomycin C on full-thickness burns.
BACKGROUND
Burns result in substantial morbidity because of fibroblast proliferation and contracture. Mitomycin C is a chemotherapeutic agent known to suppress fibroblast proliferation. It is used in ophthalmologic disorders and reduces scarring in upper aerodigestive surgery. No study of the effect of mitomycin C on cutaneous burns has been performed. This study examined burn healing in the presence of topical mitomycin C by evaluation of wound appearance, contraction, and histology in a pig model.


METHODS
Standardized full-thickness burns were produced on the flanks of three pigs. One animal received no further therapy and was an external control. Two animals underwent placement of topical mitomycin C, 0.4 mg/ml, on selected burn sites for 5 minutes. This was repeated 2 and 4 weeks after injury. Evaluation was performed at 2 and 6 months using a clinical assessment scale and a visual analogue scale. Scar length and histologic analysis were also evaluated.


RESULTS
Clinical assessment scale and visual analogue scale scores showed improved appearance in the untreated external control wounds versus the untreated internal control and treated wounds (p < 0.001). Wound contraction was not significantly different between groups. Histologic characteristics between groups were similar except for epidermal hyperplasia, which was decreased in the untreated external control (p < 0.05) at 2 months after treatment.


CONCLUSIONS
Topical mitomycin C treatment of full-thickness burn wounds at 0.4 mg/cc for three courses does not improve, and may worsen, clinical appearance and scarring during early healing. There is no difference in histology during the long-term healing process. Scar contraction was unchanged.
Tennyson
Heath
H
San Antonio, Texas; and Landstuhl, Germany From the Otolaryngology Service, Brooke Army Medical Center, Fort Sam Houston; Plastic Surgery Service, University of Texas Health Sciences Center; Otolaryngology Service, Landstuhl Regional Medical Center; Veterinary Service, U.S. Army Institute of Surgical Research, Fort Sam Houston; and Plastic Surgery Service, Audie L. Murphy Veterans Affairs Medical Center.
Helling
Eric R
ER
Wiseman
Joseph
J
Dick
Edward
E
Lyons
Robert C
RC
D000287:Administration, Topical; D000818:Animals; D002057:Burns, Chemical / Q000188:drug therapy* / Q000473:pathology; D002710:Chlorhexidine / Q000633:toxicity; D004195:Disease Models, Animal; D004202:Disinfectants / Q000633:toxicity; D005260:Female; D005500:Follow-Up Studies; D016685:Mitomycin / Q000008:administration & dosage*; D019384:Nucleic Acid Synthesis Inhibitors / Q000008:administration & dosage*; D012867:Skin / Q000293:injuries / Q000473:pathology*; D013552:Swine; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*