title: Characterization of two rice peroxidase promoters that respond to blast fungus-infection.
abstract: Peroxidase (POX) genes consist of a large gene family possibly contributing to self-defense, however constitutive and stress-induced expression patterns of individual gene were poorly understood in rice. We studied here the characteristic expression of two representative rice POX genes, R2329 and R2184, which are blast fungus-inducible (Sasaki et al. in Plant Cell Physiol 45:1442-1452, 2004). Basal GUS activity in R2329 promoter::GUS rice plants was 100-fold higher than that in R2184 promoter::GUS plants, and these levels reflected the transcript levels monitored by quantitative real-time RT-PCR. R2329 promoter was activated by blast fungus-infection and wounding, and R2184 promoter was activated by the fungal-infection and methyl jasmonate (MeJA)-treatment. By histochemical GUS staining analysis, constitutive R2329 and R2184 expression was commonly found in vascular bundle and exodermis in leaves and roots, while the precise expression profile was characteristic. In blast fungus inoculated R2329 promoter::GUS leaves, GUS staining was induced just around fungus-induced local lesions. Analysis of the 5' deleted promoters suggests the presence of many kinds of stress-responsive elements in the regions between -1798 and -748 of R2329 promoter and between -1975 and -548 of R2184 promoter. These results revealed the stress-responsive characteristics of R2329 and R2184 promoters, and indicated the possible use for generation of useful transgenic plants.
authors.affiliation: Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
authors.forename: Katsutomo
authors.initials: K
authors.lastname: Sasaki
authors.forename: Ohtsu
authors.initials: O
authors.lastname: Yuichi
authors.forename: Susumu
authors.initials: S
authors.lastname: Hiraga
authors.forename: Yoko
authors.initials: Y
authors.lastname: Gotoh
authors.forename: Shigemi
authors.initials: S
authors.lastname: Seo
authors.forename: Ichiro
authors.initials: I
authors.lastname: Mitsuhara
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ito
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Matsui
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ohashi
mesh_terms: D017343:Genes, Plant; D009181:Mycoses / Q000235:genetics*; D009928:Organ Specificity; D012275:Oryza / Q000235:genetics*; D010544:Peroxidases / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction