title: Protoplasts: preparation from higher plants.
abstract: Protoplasts can be readily prepared from higher-plant tissue with a concentrated Myrothecium cellulase preparation. An ionic osmoticum produced a higher yield and more viable protoplasts than did the nonionic man nitol osmoticum. Addition of polyuronidases or of ethylenediaminetetra-acetate did not increase the yield. Protoplasts could be prepared from root, leaf, and callus tissue as well as from coleoptiles.
authors.forename: A W
authors.initials: AW
authors.lastname: Ruesink
authors.forename: K V
authors.initials: KV
authors.lastname: Thimanndagger