title: Cytotoxicity of the novel spin trapping compound 5-ethoxycarbonyl-3,5-dimethyl-pyrroline N-oxide (3,5-EDPO) and its derivatives.
abstract: ESR spin trapping allows detection of superoxide radicals. Novel spin traps forming more stable superoxide adducts (t(1/2) ca. 12-55 min) were tested for their toxicity to cultured cells. The following toxicity ranking was obtained: 4,5-DPPO>4-BEMPO approximately 3-BEMPO>trans-3,5-EDPO>3,5-DPPO approximately 4,5-DiPPO approximately 4,5-EDPO>cis-3,5-EDPO approximately 3,5-DiPPO>DEPMPO. In conclusion, 4,5-EDPO, cis-3,5-EDPO and 3,5-DiPPO can be recommended for further investigation of superoxide in biological systems.
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria. rohr_udilova@yahoo.de
authors.forename: Nataliya
authors.initials: N
authors.lastname: Rohr-Udilova
authors.forename: Klaus
authors.initials: K
authors.lastname: Stolze
authors.forename: Sandra
authors.initials: S
authors.lastname: Sagmeister
authors.forename: Wolfram
authors.initials: W
authors.lastname: Parzefall
authors.forename: Brigitte
authors.initials: B
authors.lastname: Marian
authors.forename: Hans
authors.initials: H
authors.lastname: Nohl
authors.forename: Rolf
authors.initials: R
authors.lastname: Schulte-Hermann
authors.forename: Bettina
authors.initials: B
authors.lastname: Grasl-Kraupp
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003497:Cyclic N-Oxides / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006836:Hydro-Lyases / Q000378:metabolism; D007536:Isomerism; D015394:Molecular Structure; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D010087:Oxides / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity*; D051381:Rats; D019097:Spin Trapping; D013329:Structure-Activity Relationship; D013481:Superoxides / Q000378:metabolism