title: Autologous platelet-rich plasma as an adipocyte in vivo delivery system: case report.
abstract: Tissue engineering has emerged as a promising alternative to current clinical treatments for restoration of soft tissue defects. A key element in the process of tissue engineering is an ideal implant that provides structural support and a favorable environment for growing cells. The authors hypothesized that autologous platelet-rich plasma (APRP) could be used as an in vivo adipocyte delivery system to favor cell survival and to stimulate early recruitment of microcapillaries to the site of implantation. Autologous fat was included in APRP and injected as a gel into a subcutaneous pocket created to correct a painful, adherent scar at the shoulder level in a 75-year-old woman. The surgical outcome was evaluated by histologic and immunohistochemical analysis as well as by ecography before and after surgery. The results were satisfactory, showing fat survival 1 year after surgery. The characteristics of this new material should stimulate research into future clinical applications for such cell constructs in plastic and reconstructive surgery.
authors.affiliation: Unit of Plastic and Reconstructive Surgery, University of Padova, Via Giustiniani 2, 35100 Padova, Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Azzena
authors.forename: Francesco
authors.initials: F
authors.lastname: Mazzoleni
authors.forename: Giovanni
authors.initials: G
authors.lastname: Abatangelo
authors.forename: Barbara
authors.initials: B
authors.lastname: Zavan
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Vindigni
mesh_terms: D000273:Adipose Tissue / Q000637:transplantation*; D000368:Aged; D001792:Blood Platelets; D001804:Blood Transfusion, Autologous; D005260:Female; D006801:Humans; D019651:Plastic Surgery Procedures / Q000379:methods*; D012782:Shoulder / Q000601:surgery*; D000070599:Shoulder Injuries; D017695:Soft Tissue Injuries / Q000601:surgery; D023822:Tissue Engineering; D016896:Treatment Outcome; D014945:Wound Healing