[Association of the coding region single nucleotide polymorphisms in PLUNC gene with nasopharyngeal carcinoma].
OBJECTIVE
To investigate whether the single nucleotide polymorphism (SNP) site G14595T of PLUNC gene coding region is significantly correlated with nasopharyngeal carcinoma (NPC).


METHODS
Peripheral blood samples were collected from 239 NPC patients, 163 males and 76 females, aged 46.9, and 286 sex(-), and age(-)-matched healthy controls in Guangdong, China. The coding region and regulating region, 3'UTR and 3'Flank as well as 5'UTR and 5'Flank were sequenced to identify and characterize the SNPs. All SNP were analyzed by linkage disequilibrium (LD) and haplotype tag SNP (htSNP) derived from the results by calculating haplotype. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were used to study the PLUNC genotypes.


RESULTS
Nine SNPs were obtained by sequencing. Eight of the 9 SNPs presented high LD, and 3 of the SNP were determined as htSNPs. The frequencies of genotype and allele on SNP marker G14595T in the NPC patients and controls suggested there was no significant association between the phenotype and NPC susceptibility (P > 0.05).


CONCLUSIONS
The marker G14595T in coding region does not influence the expression of PLUNC gene. There is no significant association between the phenotype and susceptibility to NPC in Guangdong.
He
Ying
Y
Department of Otorhinolaryngology, Nanfang Hospital, Nanfang Medical University, Guangzhou 510515, China.
Ding
Yan-Qing
YQ
Huang
Zhong-Xi
ZX
Yao
Kai-Tai
KT
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide