[Comparative efficiency of detection of the causative agent of urogenital chlamydiasis by immunofluorescence, polymerase chain reaction, and dot immunoassay].
The diagnostic efficiency of three diagnostic kits--two commercial (for direct immunofluorescent test (DIFT) and polymerase chain reaction (PCR) and one predeveloped experimental dot-ELISA--was compared. For this, specimens (cervical or urethral scrapes) from 225 patients with suspected or verified urogenital chlamydiasis were analyzed. Dot-ELISA was shown to have a higher sensitivity and a higher specificity than both commercial kits. The coincidence of positive and negative results of dot-ELISA with DIFT and PCR was 98.6 +/- 1.4 and 100%, respectively. In addition, dot-ELISA on the "Amersham" membrane could detect cases of the disease by 7 and 1.2 times, as compared with PCR and DIFT, respectively. The prospects of the dot-ELISA kit for the rapid, simple, and cheap diagnosis of urogenital chlamydiasis are discussed.
Fedorova
V A
VA
Bannikova
V A
VA
Alikberov
Sh A
ShA
Eliseev
Iu Iu
IuIu
Grashkin
V A
VA
D000818:Animals; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000401:mortality; D005260:Female; D052776:Female Urogenital Diseases / Q000175:diagnosis* / Q000382:microbiology; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods; D006801:Humans; D015151:Immunoblotting; D008297:Male; D052801:Male Urogenital Diseases / Q000175:diagnosis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity