Accuracy of Burkholderia pseudomallei identification using the API 20NE system and a latex agglutination test.
In an evaluation of the API 20NE for the identification of Burkholderia spp., 792/800 (99%) Burkholderia pseudomallei and 17/19 (89%) B. cepacia isolates were correctly identified but 10 B. mallei and 98 B. thailandensis isolates were not correctly identified. A latex agglutination test was positive for 796/800 (99.5%) B. pseudomallei isolates and negative for 120 other oxidase-positive gram-negative bacilli.
Amornchai
Premjit
P
Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Chierakul
Wirongrong
W
Wuthiekanun
Vanaporn
V
Mahakhunkijcharoen
Yuvadee
Y
Phetsouvanh
Rattanaphone
R
Currie
Bart J
BJ
Newton
Paul N
PN
van Vinh Chau
Nguyen
N
Wongratanacheewin
Surasakdi
S
Day
Nicholas P J
NP
Peacock
Sharon J
SJ
D000818:Animals; D016957:Burkholderia pseudomallei / Q000302:isolation & purification*; D003951:Diagnostic Errors; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D011933:Reagent Kits, Diagnostic