Effect of scopoletin on lipoprotein lipase activity in 3T3-L1 adipocytes.
Hypertriglyceridemia is an independent risk factor of cardiovascular diseases. It is caused by the imbalance between hepatic triglyceride production and peripheral removal. Lipoprotein lipase (LPL) plays a central role in the removal of plasma triglyceride. During the screening of possible anti-dyslipidemic drugs, we observed that scopoletin (6-methoxy-7-hydroxycoumarin) significantly increased LPL activity in adipocytes. Scopoletin increased LPL activity in culture medium of 3T3-L1 adipocytes in dose- and time-dependent manners. It did not release LPL from the adipocyte membrane and, instead, increased the LPL mRNA level, suggesting transcriptional control. Scopoletin also partially reversed tumor necrosis factor-alpha-induced suppression of LPL activity. These results suggest the possible action of scopoletin as a facilitator of plasma triglyceride clearance.
Yang
Jeong-Yeh
JY
Department of Biochemistry, Medical School, Chonbuk National University, Jeonju, 561-756 Chonbuk, Korea.
Koo
Jeung-Hyun
JH
Yoon
Ha-Yong
HY
Lee
Ju-Hyung
JH
Park
Byung-Hyun
BH
Kim
Jong-Suk
JS
Chi
Myung S
MS
Park
Jin-Woo
JW
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000201:enzymology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012603:Scopoletin / Q000494:pharmacology*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha