title: CRMP3 is required for hippocampal CA1 dendritic organization and plasticity.
abstract: In vitro studies have pointed to the collapsin response mediator proteins (CRMPs) as key regulators of neurite outgrowth and axonal differentiation. CRMP3 is expressed mostly in the nervous system during development but remains at high levels in the hippocampus of adults. To explore CRMP3 function in vivo, we generated mice with targeted disruption of the CRMP3 gene. Immunohistochemistry and Golgi staining of CA1 showed abnormal dendrite and spine morphogenesis in the hippocampus of CRMP3-deficient mice. Apical dendrites displayed an increase in undulation and a reduction in length and branching points. Basal dendrites also exhibited a reduction in length with an alteration in soma stem distribution and an increased number of thick dendrites localized in stratum oriens (SO). Long-term potentiation (LTP) was impaired in this area. These data indicate an important role for CRMP3 in dendrite arborization, guide-posts navigation, and neuronal plasticity.
authors.affiliation: INSERM U842, Faculté de Médecine Laennec, Rue Guillaume Paradin, Lyon F-69372, France. quach.1@lyon.inserm.fr
authors.forename: Tam T
authors.initials: TT
authors.lastname: Quach
authors.forename: Guy
authors.initials: G
authors.lastname: Massicotte
authors.forename: Marie-Françoise
authors.initials: MF
authors.lastname: Belin
authors.forename: Jérome
authors.initials: J
authors.lastname: Honnorat
authors.forename: Erica R
authors.initials: ER
authors.lastname: Glasper
authors.forename: Anne C
authors.initials: AC
authors.lastname: Devries
authors.forename: Lyn B
authors.initials: LB
authors.lastname: Jakeman
authors.forename: Michel
authors.initials: M
authors.lastname: Baudry
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Duchemin
authors.forename: Pappachan E
authors.initials: PE
authors.lastname: Kolattukudy
mesh_terms: D000818:Animals; D048430:Cell Shape; D003712:Dendrites / Q000378:metabolism*; D004594:Electrophysiology; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D009473:Neuronal Plasticity; D013997:Time Factors