title: Chronic infections and genetic factors in the development of ischemic stroke.
abstract: The aim of this study was to examine whether chronic infections and genetic factors of the host play roles in the pathophysiology of acute noncardioembolic ischemic stroke. Blood samples from 59 subjects with ischemic stroke and 52 control patients were investigated by nested PCR for the presence of C. pneumoniae DNA, HCMV DNA and enterovirus RNA, by ELISA for the levels of antibodies to C. pneumoniae, HCMV, HSV, HHV-6, EBV and the inflammatory chemokine IL-8, and by PCR for promoter polymorphism of the IL-8 and CD14 host genes. Associations of stroke with the HCMV IgG and HSV-1 IgA antibody levels were observed. No association of stroke was detected with the presence of C. pneumoniae, HCMV or enterovirus nucleic acids in the peripheral blood, C. pneumoniae IgM, IgG and IgA, the HSV IgG, the EBV IgG, or HHV-6 IgG antibody levels, the pathogen burden, the IL-8 or CD14 promoter polymorphisms, or with the serum levels of IL-8 in the overall study population. These results are consistent with the hypothesis that certain pathogens are involved in the development of ischemic stroke.
authors.affiliation: Department of Virology, National Center for Epidemiology, H-1097 Budapest, Gyáli út 2-6, Hungary.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Kis
authors.forename: Katalin
authors.initials: K
authors.lastname: Sas
authors.forename: Zsofia
authors.initials: Z
authors.lastname: Gyulai
authors.forename: Balint
authors.initials: B
authors.lastname: Treso
authors.forename: Fruzsina
authors.initials: F
authors.lastname: Petrovay
authors.forename: Beatrix
authors.initials: B
authors.lastname: Kapusinszky
authors.forename: Marta
authors.initials: M
authors.lastname: Csire
authors.forename: Valeria
authors.initials: V
authors.lastname: Endresz
authors.forename: Katalin
authors.initials: K
authors.lastname: Burian
authors.forename: Yvette
authors.initials: Y
authors.lastname: Mandi
authors.forename: Laszlo
authors.initials: L
authors.lastname: Vecsei
authors.forename: Eva
authors.initials: E
authors.lastname: Gonczol
mesh_terms: D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood; D023521:Chlamydophila Infections / Q000150:complications / Q000175:diagnosis; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D002908:Chronic Disease; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000150:complications* / Q000175:diagnosis; D004269:DNA, Bacterial / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004770:Enterovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000150:complications / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D020022:Genetic Predisposition to Disease; D006564:Herpesviridae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000097:blood / Q000150:complications* / Q000175:diagnosis; D018259:Herpesvirus 1, Human / Q000276:immunology; D006801:Humans; D016209:Interleukin-8 / Q000097:blood / Q000235:genetics*; D007511:Ischemia / Q000097:blood / Q000209:etiology* / Q000235:genetics / Q000503:physiopathology; D018950:Lipopolysaccharide Receptors / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012307:Risk Factors; D020521:Stroke / Q000097:blood / Q000209:etiology* / Q000235:genetics / Q000503:physiopathology