title: Chromosomal mapping of the GFP-LC3 transgene in GFP-LC3 mice.
abstract: GFP-LC3 mice are widely used to monitor autophagy. Here, we report chromosomal mapping of the GFP.LC3 transgene in this mouse model. The transgene was located in a very distal region of chromosome 2, immediately upstream of a putative open reading frame that is thought to be a part of a pseudogene. With this information, we were able to generate a PCR system to easily determine zygosity. Homozygous GFP-LC3 mice (GFP-LC3(tg/tg)) were healthy, fertile and did not show an abnormal phenotype. Thus, GFP-LC3 integration in this model appears not to affect functions of other genes, justifying use of this mouse model.
authors.affiliation: Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo, Japan; SORST, Japan Science and Technology Agency, Kawaguchi, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kuma
authors.forename: Noboru
authors.initials: N
authors.lastname: Mizushima
mesh_terms: D000818:Animals; D001343:Autophagy / Q000502:physiology; D002874:Chromosome Mapping; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D019076:Transgenes