title: Creation of lipid partitions by deposition of amphipathic viral peptides.
abstract: Phospholipid vesicles exhibit a natural characteristic to fuse and reform into a continuous single bilayer membrane on hydrophilic solid substrates such as glass, mica, and silica. The resulting solid-supported bilayer mimics physiological tendencies such as lipid flip-flop and lateral mobility. The lateral mobility of fluorescently labeled lipids fused into solid-supported bilayers is found to change upon deposition on the membrane surface of an amphipathic alpha-helical peptide (AH) derived from the hepatitis C virus (HCV) NS5A protein. The binding of the AH peptide to a phospholipid bilayer, with the helical axis parallel to the bilayer, leads to immobilization of the bilayer. We used AFM to better understand the mechanistic details of this specific interaction, and determined that the diminished fluidity of the bilayer is due to membrane thinning. Utilizing this specific interaction between AH peptides and lipid molecules, we demonstrate a novel process for the creation of lipid partition by employing AH peptides as agents to immobilize lipid molecules, thus creating a patterned solid support with partition-defined areas of freely mobile lipid bilayers. This architecture could have a wide range of applications in novel sensing, biotechnology, high-throughput screening, and biomimetic strategies.
authors.affiliation: Department of Chemical Engineering, Stanford University, Stanford, California 94305, USA.
authors.forename: Nam-Joon
authors.initials: NJ
authors.lastname: Cho
authors.forename: Sang-Joon
authors.initials: SJ
authors.lastname: Cho
authors.forename: Jasper O
authors.initials: JO
authors.lastname: Hardesty
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Glenn
authors.forename: Curtis W
authors.initials: CW
authors.lastname: Frank
mesh_terms: D007700:Kinetics; D008051:Lipid Bilayers; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D017361:Viral Nonstructural Proteins / Q000737:chemistry*; D012324:RNA-Dependent RNA Polymerase