Bone transport using hydroxyapatite loaded with bone morphogenetic protein in rabbits.
The feasibility of bone transport with bone substitute and the factors which are essential for a successful bone transport are unknown. We studied six groups of 12 Japanese white rabbits. Groups A to D received cylindrical autologous bone segments and groups E and F hydroxyapatite prostheses. The periosteum was preserved in group A so that its segments had a blood supply, cells, proteins and scaffold. Group B had no blood supply. Group C had proteins and scaffold and group D had only scaffold. Group E received hydroxyapatite loaded with recombinant human bone morphogenetic protein-2 and group F had hydroxyapatite alone. Distraction osteogenesis occurred in groups A to C and E which had osteo-conductive transport segments loaded with osteo-inductive proteins. We conclude that scaffold and proteins are essential for successful bone transport, and that bone substitute can be used to regenerate bone.
Watanabe
K
K
Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takaramachi, Kanazawa, Japan.
Tsuchiya
H
H
Sakurakichi
K
K
Tomita
K
K
D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage*; D001861:Bone Regeneration / Q000187:drug effects*; D017886:Durapatite; D019857:Osteogenesis, Distraction / Q000379:methods*; D010521:Periosteum / Q000098:blood supply* / Q000502:physiology; D011817:Rabbits; D011859:Radiography; D013977:Tibia / Q000000981:diagnostic imaging / Q000601:surgery*