Interferon type I and type II responses in an Atlantic salmon (Salmo salar) SHK-1 cell line by the salmon TRAITS/SGP microarray.
Interferons (IFNs) are cytokines that have proinflammatory, antiviral, and immunomodulatory effects and play a central role during a host response to pathogens. The IFN family contains both type I and type II molecules. While there are a number of type I IFNs, there is only one type II IFN. Recently both type I and type II IFN genes have been cloned in salmonid fish and recombinant proteins produced showing IFN activity. We have stimulated an Atlantic salmon cell line (SHK-1) with both type I and type II recombinant salmonid IFNs and analyzed the transcriptional response by microarray analysis. Cells were exposed to recombinant IFNs for 6 or 24 h or left unexposed as controls. RNA was hybridized to an Atlantic salmon cDNA microarray (salmon 17K feature TRAITS/SGP array) in order to assess differential gene expression in response to IFN exposure. For IFN I and II, 47 and 72 genes were stimulated, respectively; most genes were stimulated by a single IFN type, but some were affected by both IFNs, indicating coregulation of the IFN response in fish. Real-time PCR analysis was employed to confirm the microarray results for selected differentially expressed genes in both a cell line and primary leukocyte cultures.
Martin
S A M
SA
Scottish Fish Immunology Research Centre, School of Biological Sciences, University of Aberdeen, Aberdeen, UK.
Taggart
J B
JB
Seear
P
P
Bron
J E
JE
Talbot
R
R
Teale
A J
AJ
Sweeney
G E
GE
Høyheim
B
B
Houlihan
D F
DF
Tocher
D R
DR
Zou
J
J
Secombes
C J
CJ
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D003001:Cloning, Molecular; D016207:Cytokines / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D007370:Interferon Type I / Q000235:genetics*; D007371:Interferon-gamma / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011994:Recombinant Proteins / Q000378:metabolism; D020317:Salmo salar / Q000502:physiology