[Exploratory study of novel leukemia bone marrow stromal cell adhesion mediated drug resistance model].
OBJECTIVE
To construct cell adhesion mediated drug resistance (CAM-DR) model based on Acute lymphocyte leukemia bone marrow stromal cells(BMSC) for further studying drug resistance of leukemia.


METHODS
Firstly, we adhesively cultured Jurkat cell strain of human leukaemia lymphocyte with the matrix cell radiated by (60)Co to construct the model of CAM-DR, then evaluated the model in morph and construction by scanning electron microscope. The IC50 of DNR on Jurkat cell was examen by MTT and the concentration and distribution of DNR in the cell was detected by flow cytometry.


RESULTS
When the Jurkat cells were cultured with BMSC for 24 h, we found that Jurkat cells had adhesioned the bone marrow stromal cell layer by parapodium and some of them had nichd the mesh consisted by the confluence of BMSC. At the point of 48 h, some Jurkat cells had migrated to underlayer of BMSC, and Jurkat cells were nichd the mesh of BMSC like nidi. The accumulation and distribution of DNR in the Jurkat cells were not affected in the model, but the reaction of Jurkat cells to DNR were significantly inhibited.


CONCLUSIONS
The model of CAM-DR based on Acute lymphocyte leukemia bone marrow stromal cells was successfully built.
Chen
Xing-hua
XH
Hematology Department of Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. xhchen888@yahoo.com.cn
Gao
Lei
L
Zhang
Xi
X
Wang
Ji-gang
JG
Zhang
Cheng
C
Peng
Xian-gui
XG
Li
Zhong-jun
ZJ
Zeng
Dong-feng
DF
Liu
Yao
Y
Si
Ying-jian
YJ
D000293:Adolescent; D000328:Adult; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000473:pathology* / Q000648:ultrastructure; D002448:Cell Adhesion; D002470:Cell Survival / Q000187:drug effects; D002648:Child; D002675:Child, Preschool; D003630:Daunorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D020128:Inhibitory Concentration 50; D019169:Jurkat Cells; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood / Q000473:pathology*; D017154:Stromal Cells / Q000473:pathology* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured