[SC35 splicing factor and coilin are colocalized within the "endobodies" in oocytes of the spider Araneus diadematus].
Oocytes of the spider Araneus diadematus are solitary developed. The oocyte nuclei are transcriptionally active, as revealed using microinjections of 5-bromouridine 5'-triphosphate into the ooplasm. The nucleus contains several extrachromosomal structures of perfectly spherical shape, the so-called endobodies. Laser scanning confocal microscopy revealed intense fluorescence of endobodies when an antibody against the splicing factor SC35, a major component of interchromatin granule clusters (IGCs), was applied. At the same time, colocalization of SC35 protein with coilin, a marker protein of Cajal bodies (CBs), was also observed within these structure. We suggest that endobodies of A. diadematus oocytes may have some features of both IGCs and CBs and represent a single nuclear domain.
Bogoliubov
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Bogoliubova
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D000818:Animals; D000911:Antibodies, Monoclonal; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D022003:Cell Nucleus Structures / Q000378:metabolism / Q000648:ultrastructure; D020541:Coiled Bodies / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods; D018613:Microscopy, Confocal / Q000379:methods; D009687:Nuclear Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism / Q000648:ultrastructure*; D012326:RNA Splicing; D013112:Spiders; D017381:Spliceosomes / Q000378:metabolism / Q000648:ultrastructure