title: Comparison of a commercial and an in-house T cell-based assay for the diagnosis of Mycobacterium tuberculosis infection.
abstract: Identification of individuals with a tuberculosis infection is a very important element for the control of tuberculosis. The currently used tuberculin skin test has poor sensitivity and specificity. Recently, an important advance in tuberculosis diagnosis occurred with the development of in vitro T cell-based IFN-gamma release assays. The aim of this study was to compare a RD1-based in-house ELISPOT-IFN-gamma assay with a commercial (T-SPOT.TB) assay for the diagnosis of tuberculosis infection. The results showed an almost complete concordance between the two assays, confirming that our restricted but highly selected pool of peptides is sufficient to detect tuberculosis infection.
authors.affiliation: Clinic of Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Mantegani
authors.forename: Federica
authors.initials: F
authors.lastname: Piana
authors.forename: Laura
authors.initials: L
authors.lastname: Galli
authors.forename: Paolo
authors.initials: P
authors.lastname: Scarpellini
authors.forename: Luigi
authors.initials: L
authors.lastname: Codecasa
authors.forename: Adriano
authors.initials: A
authors.lastname: Lazzarin
authors.forename: Daniela
authors.initials: D
authors.lastname: Cirillo
authors.forename: Claudio
authors.initials: C
authors.lastname: Fortis
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007223:Infant; D007371:Interferon-gamma / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013601:T-Lymphocytes / Q000276:immunology; D014376:Tuberculosis / Q000097:blood / Q000175:diagnosis*