title: Cultivation of the Drosophila sex-ratio spiroplasma.
abstract: Uncultivable for more than 25 years, the sex-ratio spiroplasma of Drosophila willistoni grew in a tissue culture medium (H-2) containing an embryo-derived lepidopteran cell line (IPLB-TN-R(2)). After adaptation, it grew in a cell-free H-2 medium. This success demonstrates the usefulness of cell culture systems for cultivation of fastidious microorganisms and facilitates study of the sex-ratio trait in Drosophila.
authors.forename: K J
authors.initials: KJ
authors.lastname: Hackett
authors.forename: D E
authors.initials: DE
authors.lastname: Lynn
authors.forename: D L
authors.initials: DL
authors.lastname: Williamson
authors.forename: A S
authors.initials: AS
authors.lastname: Ginsberg
authors.forename: R F
authors.initials: RF
authors.lastname: Whitcomb