Comparison of the kinetics of iron release from a marine (Trichodesmium erythraeum) Dps protein and mammalian ferritin in the presence and absence of ligands.
The ferritin superfamily of iron storage proteins includes ferritin proper and Dps (DNA binding protein from starved cells) along with bacterioferritin. We examined the release of Fe from the Dps of Trichodesmium erythraeum (Dps(tery)) and compared it to the release of Fe from horse spleen ferritin (HoSF) under various conditions. Both desferrioxamine B (DFB), a Fe(III) chelator, and ascorbic acid were able to mobilize Fe from Dps(tery) at rates comparable to those observed for HoSF. The initial Fe release rate from both proteins increased linearly with the concentration of DFB, suggesting that the chelator binds to Fe in the protein. A small but significant rate obtained by extrapolation to zero concentration of DFB implies that Dps(tery) and HoSF might release Fe(III) spontaneously. A similar result was observed for HoSF in the presence of sulfoxine. In a different experiment, Fe(III) was transferred from holoferritin to apotransferrin across a dialysis membrane in the absence of chelator or reducing agent. The apparent spontaneous release of Fe from HoSF and Dps(tery) brings forth the hypothesis that the Fe core in Fe storage proteins might be continuously dissolving and re-precipitating in vivo, thus maintaining it in a highly reactive and bioavailable form.
Castruita
Madeli
M
Department of Chemistry, Princeton University, Princeton, NJ 08540, USA. madeli@chem.ucla.edu
Elmegreen
Lauren A
LA
Shaked
Yeala
Y
Stiefel
Edward I
EI
Morel
François M M
FM
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D000458:Cyanobacteria / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D003676:Deferoxamine / Q000494:pharmacology; D005293:Ferritins / Q000737:chemistry / Q000378:metabolism*; D006736:Horses; D007501:Iron / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D010084:Oxidation-Reduction / Q000187:drug effects; D013154:Spleen / Q000378:metabolism