title: Comparison of plaque- and flow cytometry-based methods for measuring dengue virus neutralization.
abstract: As dengue vaccines enter clinical trials, there is a need for rapid and quantitative assays to measure neutralization. We have developed flow-based neutralization assays which generated results similar to those generated by the established, plaque reduction neutralization test. The flow assays are an improvement, as they use human cells and allow for high-throughput screening.
authors.affiliation: Department of Microbiology and Immunology, CB#7290, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Annette A
authors.initials: AA
authors.lastname: Kraus
authors.forename: William
authors.initials: W
authors.lastname: Messer
authors.forename: Laura B
authors.initials: LB
authors.lastname: Haymore
authors.forename: Aravinda M
authors.initials: AM
authors.lastname: de Silva
mesh_terms: D053059:Dengue Vaccines / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D009500:Neutralization Tests / Q000379:methods*; D010948:Viral Plaque Assay