[Determination of three glycosides from herbs of Swertia punicea by RP-HPLC].
OBJECTIVE
To develop a RP-HPLC method for determination of three glycosides in Swertia punicea.


METHODS
Chromatographic column: Alltimal C18 (4.6 mm x 250 mm, 5 microm). Mobile phase: methanol-water (including 0.05% H3PO4), and gradient elution. Flow rate: 1 mL x min(-1). Wavelength: 254 nm. Column temperture: 30 degrees C.


RESULTS
The calibration curves of gentiopicroside, mangiferin and swertrianolin were in good linearity over the range of 31.3-281.7, 0.31-2.78, 0.55-4.91 microg, (r = 0.9996, 0.9993, 0.9995). The average recoveries were 103.36%, 101.42% and 97.39%, with RSD less then 3% (n = 5).


CONCLUSIONS
It is a simple and sensitive meathod in controlling the quality of S. punicea.
Zhang
Xiu-qiao
XQ
Hubei College of Traditional Chinese Medicine, Wuhan 430061, China.
Huang
Feng-jiao
FJ
Chen
Jiao-chun
JC
Liu
Yan-wen
YW
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000592:standards; D005960:Glucosides / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D057888:Iridoid Glucosides; D039823:Iridoids / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D029967:Swertia / Q000737:chemistry*; D044004:Xanthones / Q000032:analysis*