Candida albicans THI13 disruption affects production of monocytic cytokines.
BACKGROUND
The Candida albicansTHI13 gene was identified by its homology to the Candida tropicalis CtNMT1 gene, which is involved in pyrimidine precursor biosynthesis.


METHODS
Disruption of THI13 revealed that this gene played a minor role in thiamin biosynthesis in C. albicans. Purified human monocytes were incubated with C. albicans at the optimal Candida: monocyte ratio of 0.5 and cytokines in the supernatants were measured by enzyme-linked immunosorbent assay.


CONCLUSIONS
This experiment showed that the wild-type strain significantly induced interleukin-10 (IL-10) production but had little effect on IL-12 production, and that THI13 mutants had no significant effect on IL-10 production, though the IL-12 level was increased in the supernatants. These results suggest that THI13 is involved in the host effective immune response by regulating IL-10 and IL-12 production.
Zhu
Z Y
ZY
School of Pharmacy, Second Military Medical University, Shanghai, China.
Cao
Y Y
YY
Wang
Y
Y
Quan
H
H
Zhao
J X
JX
Jia
X M
XM
Cao
Y B
YB
Gao
P H
PH
Xu
Z
Z
Jiang
Y Y
YY
D002176:Candida albicans / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics*; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology; D018664:Interleukin-12 / Q000096:biosynthesis / Q000276:immunology; D007378:Interleukins / Q000096:biosynthesis / Q000235:genetics*; D009000:Monocytes / Q000276:immunology*; D009154:Mutation / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013831:Thiamine / Q000096:biosynthesis / Q000235:genetics*