title: Effect of scopoletin on lipoprotein lipase activity in 3T3-L1 adipocytes.
abstract: Hypertriglyceridemia is an independent risk factor of cardiovascular diseases. It is caused by the imbalance between hepatic triglyceride production and peripheral removal. Lipoprotein lipase (LPL) plays a central role in the removal of plasma triglyceride. During the screening of possible anti-dyslipidemic drugs, we observed that scopoletin (6-methoxy-7-hydroxycoumarin) significantly increased LPL activity in adipocytes. Scopoletin increased LPL activity in culture medium of 3T3-L1 adipocytes in dose- and time-dependent manners. It did not release LPL from the adipocyte membrane and, instead, increased the LPL mRNA level, suggesting transcriptional control. Scopoletin also partially reversed tumor necrosis factor-alpha-induced suppression of LPL activity. These results suggest the possible action of scopoletin as a facilitator of plasma triglyceride clearance.
authors.affiliation: Department of Biochemistry, Medical School, Chonbuk National University, Jeonju, 561-756 Chonbuk, Korea.
authors.forename: Jeong-Yeh
authors.initials: JY
authors.lastname: Yang
authors.forename: Jeung-Hyun
authors.initials: JH
authors.lastname: Koo
authors.forename: Ha-Yong
authors.initials: HY
authors.lastname: Yoon
authors.forename: Ju-Hyung
authors.initials: JH
authors.lastname: Lee
authors.forename: Byung-Hyun
authors.initials: BH
authors.lastname: Park
authors.forename: Jong-Suk
authors.initials: JS
authors.lastname: Kim
authors.forename: Myung S
authors.initials: MS
authors.lastname: Chi
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Park
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000201:enzymology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012603:Scopoletin / Q000494:pharmacology*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha