Evaluation of protein farnesyltransferase substrate specificity using synthetic peptide libraries.
Farnesylation, catalyzed by protein farnesyltransferase (FTase), is an important post-translational modification guiding cellular localization. Recently predictive models for identifying FTase substrates have been reported. Here we evaluate these models through screening of dansylated-GCaaS peptides, which also provides new insights into the protein substrate selectivity of FTase.
Krzysiak
Amanda J
AJ
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, 575 Stadium Mall Drive, West Lafayette, IN 47907, USA.
Scott
Sarah A
SA
Hicks
Katherine A
KA
Fierke
Carol A
CA
Gibbs
Richard A
RA
D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D051231:Farnesyltranstransferase / Q000378:metabolism*; D015394:Molecular Structure; D019151:Peptide Library; D013379:Substrate Specificity