title: Isolation of cerebroside containing glucose (Glucosyl ceramide) and its possible significance in ganglioside synthesis.
abstract: A small amount of cerebroside containing glucose (glucosyl ceramide) was isolated from bovine brain by Florisil column chromatography and thin-layer chromatography. The fatty acids of the glucosyl ceramide were palmitic and stearic acids; small amounts of oleic and linoleic acids were present.Rat brain tissue slices, incubated with U-(14)C-glucose, incorporated more radioacivity into glucosyl ceramide than into galactosyl ceramide. From these results the possible metabolic significance of the brain glucosyl ceramide in ganglioside metabolism is discussed.
authors.affiliation: Department of Biochemistry, Institute of Medical Science, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nishimura
authors.forename: T
authors.initials: T
authors.lastname: Yamakawa