Screening of endothelial expression libraries for the identification of novel autoantigens involved in distinct autoimmune diseases characterized by endothelial dysfunction.
Screening a cDNA expression library is a powerful technique that allows identification of previously uncharacterized antigens. Proteins recognized by antibodies from patients with autoimmune diseases have been intensively studied over the past two decades since cDNAs encoding autoantigens have become available. Identity of many of them has been defined, and specific structural motifs or post-translational modifications, which may be important to explain the generation of such antibodies during the autoimmune process, have been pointed out. The screening of human umbilical artery or microvascular endothelial cell expression libraries appears to be a useful tool for the characterization of endothelial autoantigens allowing us to identify several molecules recognized from serum anti-endothelial cell antibodies of patients with diseases characterized by immune-mediated endothelial dysfunctions.
Margutti
Paola
P
Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Rome, Italy.
Delunardo
Federica
F
Colasanti
Tania
T
Piro
Ettore
E
Profumo
Elisabetta
E
Riganò
Rachele
R
Siracusano
Alessandra
A
Buttari
Brigitta
B
Conti
Fabrizio
F
Alessandri
Cristiano
C
Capozzi
Antonella
A
Sorice
Maurizio
M
Ortona
Elena
E
D001324:Autoantigens / Q000276:immunology*; D001327:Autoimmune Diseases / Q000175:diagnosis* / Q000276:immunology* / Q000378:metabolism; D001528:Behcet Syndrome / Q000276:immunology; D002340:Carotid Artery Diseases / Q000276:immunology; D030561:Databases, Nucleic Acid; D042783:Endothelial Cells / Q000276:immunology* / Q000378:metabolism*; D005820:Genetic Testing / Q000379:methods*; D006801:Humans