Chronic infections and genetic factors in the development of ischemic stroke.
The aim of this study was to examine whether chronic infections and genetic factors of the host play roles in the pathophysiology of acute noncardioembolic ischemic stroke. Blood samples from 59 subjects with ischemic stroke and 52 control patients were investigated by nested PCR for the presence of C. pneumoniae DNA, HCMV DNA and enterovirus RNA, by ELISA for the levels of antibodies to C. pneumoniae, HCMV, HSV, HHV-6, EBV and the inflammatory chemokine IL-8, and by PCR for promoter polymorphism of the IL-8 and CD14 host genes. Associations of stroke with the HCMV IgG and HSV-1 IgA antibody levels were observed. No association of stroke was detected with the presence of C. pneumoniae, HCMV or enterovirus nucleic acids in the peripheral blood, C. pneumoniae IgM, IgG and IgA, the HSV IgG, the EBV IgG, or HHV-6 IgG antibody levels, the pathogen burden, the IL-8 or CD14 promoter polymorphisms, or with the serum levels of IL-8 in the overall study population. These results are consistent with the hypothesis that certain pathogens are involved in the development of ischemic stroke.
Kis
Zoltan
Z
Department of Virology, National Center for Epidemiology, H-1097 Budapest, Gyáli út 2-6, Hungary.
Sas
Katalin
K
Gyulai
Zsofia
Z
Treso
Balint
B
Petrovay
Fruzsina
F
Kapusinszky
Beatrix
B
Csire
Marta
M
Endresz
Valeria
V
Burian
Katalin
K
Mandi
Yvette
Y
Vecsei
Laszlo
L
Gonczol
Eva
E
D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood; D023521:Chlamydophila Infections / Q000150:complications / Q000175:diagnosis; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D002908:Chronic Disease; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000150:complications* / Q000175:diagnosis; D004269:DNA, Bacterial / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004770:Enterovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000150:complications / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D020022:Genetic Predisposition to Disease; D006564:Herpesviridae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000097:blood / Q000150:complications* / Q000175:diagnosis; D018259:Herpesvirus 1, Human / Q000276:immunology; D006801:Humans; D016209:Interleukin-8 / Q000097:blood / Q000235:genetics*; D007511:Ischemia / Q000097:blood / Q000209:etiology* / Q000235:genetics / Q000503:physiopathology; D018950:Lipopolysaccharide Receptors / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012307:Risk Factors; D020521:Stroke / Q000097:blood / Q000209:etiology* / Q000235:genetics / Q000503:physiopathology