Choline transporters in human lung adenocarcinoma: expression and functional implications.
Choline is an essential nutrient for cell survival and proliferation, however, the expression and function of choline transporters have not been well identified in cancer. In this study, we detected the mRNA and protein expression of organic cation transporter OCT3, carnitine/cation transporters OCTN1 and OCTN2, and choline transporter-like protein CTL1 in human lung adenocarcinoma cell lines A549, H1299 and SPC-A-1. Their expression pattern was further confirmed in 25 human primary adenocarcinoma tissues. The choline uptake in these cell lines was significantly blocked by CTL1 inhibitor, but only partially inhibited by OCT or OCTN inhibitors. The efficacy of these inhibitors on cell proliferation is closely correlated with their abilities to block choline transport. Under the native expression of these transporters, the total choline uptake was notably blocked by specific PI3K/AKT inhibitors. These results describe the expression of choline transporters and their relevant function in cell proliferation of human lung adenocarcinoma, thus providing a potential choline-starvation strategy of cancer interference through targeting choline transporters, especially CTL1.
Wang
Tao
T
Medical College of Fudan University, Shanghai 200032, China.
Li
Jinjun
J
Chen
Fei
F
Zhao
Yingjun
Y
He
Xianghuo
X
Wan
Dafang
D
Gu
Jianren
J
D000230:Adenocarcinoma / Q000378:metabolism*; D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D045744:Cell Line, Tumor; D002794:Choline / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D027701:Organic Cation Transport Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D014407:Tumor Cells, Cultured