title: Repeat organization and epigenetic regulation of the DH-Cmu domain of the immunoglobulin heavy-chain gene locus.
abstract: The first steps of murine immunoglobulin heavy-chain (IgH) gene recombination take place within a chromosomal domain that contains diversity (D(H)) and joining (J(H)) gene segments, but not variable (V(H)) gene segments. Here we show that the chromatin state of this domain is markedly heterogeneous. Specifically, only 5'- and 3'-most D(H) gene segments carry active chromatin modifications, whereas intervening D(H)s are associated with heterochromatic marks that are maintained by ongoing histone deacetylation. The intervening D(H)s form part of a tandemly repeated sequence that expresses tissue-specific, antisense oriented transcripts. We propose that the intervening D(H) genes are actively suppressed by repeat-induced epigenetic silencing, which is reflected in their infrequent representation in DJ(H) junctions compared to the flanking D(H) genes.
authors.affiliation: Laboratory of Cellular and Molecular Biology, National Institute on Aging, Baltimore, MD 21224, USA.
authors.forename: Tirtha
authors.initials: T
authors.lastname: Chakraborty
authors.forename: Dipanjan
authors.initials: D
authors.lastname: Chowdhury
authors.forename: Amanda
authors.initials: A
authors.lastname: Keyes
authors.forename: Anant
authors.initials: A
authors.lastname: Jani
authors.forename: Ramesh
authors.initials: R
authors.lastname: Subrahmanyam
authors.forename: Irina
authors.initials: I
authors.lastname: Ivanova
authors.forename: Ranjan
authors.initials: R
authors.lastname: Sen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D044127:Epigenesis, Genetic; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D050438:Genes, Immunoglobulin Heavy Chain; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000235:genetics; D051379:Mice; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D011995:Recombination, Genetic; D020080:Tandem Repeat Sequences