title: Preparation and properties of an apoprotein derivative of human serum beta-lipoprotein.
abstract: The aim of this study was to develop a convenient method for the preparation and study of a soluble delipidated form of human serum beta-lipoprotein. This was achieved by succinylation and delipidation with ether-ethanol (3ratio1). The succinylated apoprotein was soluble in either 0.13 M Tris-HCl buffer, pH 8.2 (for beta-lipoprotein prepared by ultracentrifugation) or in the same Tris buffer to which 5 mM sodium decyl sulfate was added (for heparin-Mn precipitated beta-lipoprotein). The immunological activity of beta-lipoprotein or its apoprotein were markedly altered by succinylation. Whereas the succinylated beta-lipoprotein appeared as one peak in the analytical ultracentrifuge, the succinylated apoprotein appeared as two. Under the electron microscope beta-lipoprotein and succinylated beta-lipoprotein were indistinguishable, appearing as uniform preparations of spherical particles 215 to 220 A in diamater.
authors.affiliation: Laboratory of Molecular Diseases, National Heart Institute, National Institutes of Health, 20014, Bethesda, Maryland.
authors.forename: A M
authors.initials: AM
authors.lastname: Gotto
authors.forename: R I
authors.initials: RI
authors.lastname: Levy
authors.forename: D S
authors.initials: DS
authors.lastname: Fredrickson