Comparison of plaque- and flow cytometry-based methods for measuring dengue virus neutralization.
As dengue vaccines enter clinical trials, there is a need for rapid and quantitative assays to measure neutralization. We have developed flow-based neutralization assays which generated results similar to those generated by the established, plaque reduction neutralization test. The flow assays are an improvement, as they use human cells and allow for high-throughput screening.
Kraus
Annette A
AA
Department of Microbiology and Immunology, CB#7290, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
Messer
William
W
Haymore
Laura B
LB
de Silva
Aravinda M
AM
D053059:Dengue Vaccines / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D009500:Neutralization Tests / Q000379:methods*; D010948:Viral Plaque Assay