Functional polymorphisms in the promoter region of macrophage migration inhibitory factor and chronic gastritis.
Macrophage migration inhibitory factor (MIF) is a key proinflammatory mediator, which plays a pivotal role in inflammatory and immune diseases. We attempted to clarify associations of the functional polymorphisms of the MIF gene promoter with the development of chronic gastritis. The study was performed with 290 stocked DNAs from subjects with no evidence of gastric malignancy. We employed the PCR-SSCP method to detect gene polymorphisms. The severity of histological chronic gastritis in antral biopsy specimens was classified according to the updated Sydney system. Both the 7/7-CATT repeat at position -794 and the -173 C/C genotypes were significantly associated with a risk of developing severe gastric mucosal atrophy (OR, 9.69; 95% CI, 1.29-72.5; and OR, 4.60; 95% CI, 1.05-20.2, respectively). In subjects younger than 60 years old, the number of 7-CATT alleles was significantly correlated with both the activity and inflammation scores (p=0.0079 and 0.0080, respectively). Our results suggested that functional promoter polymorphisms of the MIF gene might be associated with the severity of gastric mucosal inflammation in younger subjects and with the subsequent development of mucosal atrophy.
Arisawa
Tomiyasu
T
Department of Gastroenterology, Fujita Health University School of Medicine, Toyoake 470-1192, Japan. tarisawa@fujita-hu.ac.jp
Tahara
Tomomitsu
T
Shibata
Tomoyuki
T
Nagasaka
Mitsuo
M
Nakamura
Masakatsu
M
Kamiya
Yoshio
Y
Fujita
Hiroshi
H
Nakamura
Masahiko
M
Yoshioka
Daisuke
D
Arima
Yuko
Y
Okubo
Masaaki
M
Hirata
Ichiro
I
Nakano
Hiroshi
H
D001284:Atrophy; D002465:Cell Movement; D002908:Chronic Disease; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology; D005756:Gastritis / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006801:Humans; D007249:Inflammation; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012307:Risk Factors