title: Ex vivo supplementation of TGF-beta1 enhances the fibrous tissue regeneration effect of synovium-derived fibroblast transplantation in a tendon defect: a biomechanical study.
abstract: The present study was conducted to test a hypothesis that the ex vivo supplementation of TGF-beta1 into medium will significantly improve the mechanical properties of the fibrous tissue regenerated in the patellar tendon defect after transplantation of cultured autologous synovium-derived fibroblasts. Thirty rabbits were divided into the following three groups. In Group A, we applied phosphate buffered saline of 0.1 ml to the defect created in the patellar tendon. In Group B, we transplanted autologous fibroblasts, which had been cultured into the tendon defect. In Group C, we transplanted autologous fibroblasts, which had been cultured with supplementation of TGF-beta1, into the tendon defect. Animals were killed at 6 weeks, and the regenerated tissue was examined for biomechanics and histology. The tangent modulus and the tensile strength of Group C were significantly higher than that of Group B, while the tensile strength of Group C was significantly lower than that of Group A. Histologically, vascular formation was abundantly found in the regenerated tissue of Groups B and C as compared to the regenerated tissues in Group A. The present study showed that transplantation of cultured autologous synovium-derived fibroblasts enhanced vascular formation in the fibrous tissue regenerated in the patellar tendon defect, while cell transplantation deteriorated the mechanical properties of the regenerated fibrous tissue. However, the ex vivo supplementation of TGF-beta1 into the medium significantly decreased mechanical deterioration of the fibrous tissue regenerated in the tendon defect after transplantation of cultured autologous synovium-derived fibroblasts.
authors.affiliation: Department of Sports Medicine, Hokkaido University School of Medicine, Kita-15 Nishi-7, Kita-ku, Sapporo 060-8638, Japan.
authors.forename: Shoichi
authors.initials: S
authors.lastname: Okamoto
authors.forename: Harukazu
authors.initials: H
authors.lastname: Tohyama
authors.forename: Eiji
authors.initials: E
authors.lastname: Kondo
authors.forename: Yuko
authors.initials: Y
authors.lastname: Anaguchi
authors.forename: Shin
authors.initials: S
authors.lastname: Onodera
authors.forename: Kozaburo
authors.initials: K
authors.lastname: Hayashi
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yasuda
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D004195:Disease Models, Animal; D005260:Female; D005347:Fibroblasts / Q000637:transplantation*; D017847:Patellar Ligament / Q000293:injuries; D011817:Rabbits; D012038:Regeneration; D013708:Tendon Injuries / Q000628:therapy*; D013710:Tendons / Q000502:physiology*; D053773:Transforming Growth Factor beta1 / Q000502:physiology*; D019737:Transplants