Detection of Encephalitozoon cuniculi in a new host--cockateel (Nymphicus hollandicus) using molecular methods.
A total of 123 avian faecal specimens randomly collected in Bohemian commercial aviaries, Zoo parks and countryside were screened for the presence of human pathogenic microsporidia by both calcofluor M2R staining and polymerase chain reaction. Of these, no positive sample was detected using microscopical examination, and one isolate was detected by polymerase chain reaction and identified as Encephalitozoon cuniculi. Cockateel (Nymphicus hollandicus) represents a new avian host of this microsporidian.
Kasicková
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Faculty of Biological Science, University of South Bohemia, Ceské Budejovice, Czech Republic. denisa.k@centrum.cz
Sak
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B
Kvác
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Ditrich
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O
D000818:Animals; D001715:Bird Diseases / Q000382:microbiology*; D046410:Cockatoos / Q000382:microbiology*; D004271:DNA, Fungal / Q000032:analysis* / Q000302:isolation & purification; D009670:Encephalitozoon cuniculi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016890:Encephalitozoonosis / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA