Expression of a buckwheat trypsin inhibitor gene in Escherichia coli and its effect on multiple myeloma IM-9 cell proliferation.
The gene of buckwheat trypsin inhibitor (BTI) has been cloned and expressed in Escherichia coli. The yield of this recombinant inhibitor was over 12 mg/L by using one-step purification on a Ni2+-NTA Sepharose column. Its molecular weight was 9322.1 Da, determined by mass spectrum analysis. The MTT and cytometry analyses showed that recombinant BTI could specifically inhibit the proliferation of IM-9 human B lymphoblastoid cells (from patient with multiple myeloma) in a dose-dependent manner. The test of recombinant BTI-induced apoptosis in IM-9 cells implied that the inhibitor might have potential application in the treatment of cancer.
Zhang
Zheng
Z
Key Laboratory for Chemical Biology and Molecular Engineering of the Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China.
Li
Yuying
Y
Li
Chen
C
Yuan
Jingming
J
Wang
Zhuanhua
Z
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004926:Escherichia coli / Q000235:genetics; D019612:Fagopyrum / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D006801:Humans; D009101:Multiple Myeloma / Q000378:metabolism* / Q000473:pathology*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology