Structural and analytical studies of silica accumulations in Equisetum hyemale.
Horsetail (Equisetum spp.) is known as one of the strongest accumulators of silicon among higher terrestrial plants. We use the combination of position-resolved analytical techniques, namely microtomography, energy-dispersive X-Ray elemental mapping, Raman microscopy, as well as small-angle and wide-angle scattering of X-rays, to study the type, distribution and nanostructure of silica in the internodes of Equisetum hyemale. The predominant silicification pattern is a thin continuous layer on the entire outer epidermis with the highest density in particular knob regions of the long epidermal cells. The knob tips contain up to 33 wt% silicon in the form of pure hydrated amorphous silica, while the silica content is lower in the inner part of the knobs and on the continuous layer. In contrast to the knob tips, the silica in these regions lacks silanol groups and is proposed to be in close association with polysaccharides. No mentionable amount of crystalline silica is detected by wide-angle X-ray scattering. The small-angle X-ray scattering data are consistent with the presence of colloidal, sheet-like silica agglomerates with a thickness of about 2 nm. From these results we conclude that there are at least two distinct forms of silica in E. hyemale which may have different functions. The close association of silica with cell wall polymers suggests that they may act as a polymeric template that controls the shape and size of the colloidal silica particles similar to many other biominerals and mineralised tissues. We propose that owing to its specific distribution in E. hyemale, a protective role and possibly also an important biomechanical role are among the most likely functions of silica in these plants.
Sapei
Lanny
L
Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476, Potsdam-Golm, Germany.
Gierlinger
Notburga
N
Hartmann
Jürgen
J
Nöske
Robert
R
Strauch
Peter
P
Paris
Oskar
O
D004577:Electron Probe Microanalysis; D020829:Equisetum / Q000737:chemistry* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D019441:Plant Epidermis / Q000737:chemistry / Q000378:metabolism; D018547:Plant Stems / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D012542:Scattering, Radiation; D012822:Silicon Dioxide / Q000032:analysis* / Q000378:metabolism; D013059:Spectrum Analysis, Raman; D014056:Tomography, X-Ray; D014965:X-Rays