Separation of cholesterol-desmosterol acetates by thin-layer and column chromatography on silica gel G-silver nitrate.
A chromatographic method, using columns packed with Celite-Silica Gel G impregnated with silver nitrate, has been developed for the separation of cholesterol from desmosterol (24-dehydrocholesterol).The system employed allows a complete separation of the two sterols as acetates, as determined by gas-liquid chromatography and thin-layer chromatography.The recovery of pure cholesterol and desmosterol is quantitative.
Galli
G
G
Institute of Pharmacology, University of Milan, Milan, Italy.
Paoletti
E G
EG