title: Lattice simulations of cotranslational folding of single domain proteins.
abstract: We use lattice protein models and Monte Carlo simulations to study cotranslational folding of small single domain proteins. We show that the assembly of native structure begins during late extrusion stages, but final formation of native state occurs during de novo folding, when all residues are extruded. There are three main results in our study. First, for the sequences displaying two-state refolding mechanism de novo cotranslational folding pathway differs from that sampled in in vitro refolding. The change in folding pathways is due to partial assembly of native interactions during extrusion that results in different starting conditions for in vitro refolding and for de novo cotranslational folding. For small single domain proteins cotranslational folding is slower than in vitro refolding, but is generally fast enough to be completed before the release from a ribosome. Second, we found that until final stages of biosynthesis cotranslational folding is essentially equilibrium. This observation is explained by low stability of structured states for partially extruded chains. Finally, our data suggest that the proteins, which refold in vitro slowly via intermediates, complete their de novo folding after the release from a ribosome. Comparison of our lattice cotranslational simulations with recent experimental and computational studies is discussed.
authors.affiliation: Department of Bioinformatics and Computational Biology, George Mason University, Manassas, Virginia 20110, USA.
authors.forename: Peiyu
authors.initials: P
authors.lastname: Wang
authors.forename: Dmitri K
authors.initials: DK
authors.lastname: Klimov
mesh_terms: D001665:Binding Sites; D003198:Computer Simulation; D007700:Kinetics; D009010:Monte Carlo Method; D010446:Peptide Fragments / Q000737:chemistry; D011487:Protein Conformation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry