title: Medicago species affect the community composition of arbuscular mycorrhizal fungi associated with roots.
abstract: Sequencing of the 5' end of the large ribosomal subunit (LSU rDNA) and quantitative polymerase chain reaction (qPCR) were combined to assess the impact of four annual Medicago species (Medicago laciniata, Medicago murex, Medicago polymorpha and Medicago truncatula) on the genetic diversity of arbuscular mycorrhizal (AM) fungi, and on the relative abundance of representative AM fungal genotypes, in a silty-thin clay soil (Mas d'Imbert, France). Two hundred and forty-six Glomeromycete LSU rDNA sequences from the four plant species and the bulk soil were analysed. The high bootstrap values of the phylogenetic tree obtained allowed the delineation of 12 operational taxonomic units (OTUs), all belonging to Glomus. Specific primers targeting Glomeromycetes and major OTUs were applied to quantify their abundance by qPCR. Glomeromycetes and targeted OTUs were significantly more abundant in the root tissues than in the bulk soil, and the frequencies of three of them differed significantly in the root tissues of the different plant species. These differences indicate that, despite the absence of strict host specificity in mycorrhizal symbiosis, there was a preferential association between some AM fungal and plant genotypes.
authors.affiliation: INRA, Université de Bourgogne, UMR1229 Microbiologie du Sol et de l'Environnement, CMSE, 17 rue Sully, BP 86510, F-21065 Dijon cedex, France.|Università del Piemonte Orientale 'Amedeo Avogadro', Dipartimento di Scienze dell'Ambiente e della Vita, via Bellini 25/G, 15100 Alessandria, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Pivato
authors.affiliation: INRA, Université de Bourgogne, UMR1229 Microbiologie du Sol et de l'Environnement, CMSE, 17 rue Sully, BP 86510, F-21065 Dijon cedex, France.
authors.forename: S
authors.initials: S
authors.lastname: Mazurier
authors.affiliation: INRA, Université de Bourgogne, UMR1229 Microbiologie du Sol et de l'Environnement, CMSE, 17 rue Sully, BP 86510, F-21065 Dijon cedex, France.
authors.forename: P
authors.initials: P
authors.lastname: Lemanceau
authors.affiliation: INRA, Université de Bourgogne, UMR1229 Microbiologie du Sol et de l'Environnement, CMSE, 17 rue Sully, BP 86510, F-21065 Dijon cedex, France.
authors.forename: S
authors.initials: S
authors.lastname: Siblot
authors.affiliation: Università del Piemonte Orientale 'Amedeo Avogadro', Dipartimento di Scienze dell'Ambiente e della Vita, via Bellini 25/G, 15100 Alessandria, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Berta
authors.affiliation: INRA, Université de Bourgogne, UMR1229 Microbiologie du Sol et de l'Environnement, CMSE, 17 rue Sully, BP 86510, F-21065 Dijon cedex, France.
authors.forename: C
authors.initials: C
authors.lastname: Mougel
authors.affiliation: INRA, CNRS, Université de Bourgogne, UMR Plante-Microbe-Environnement, CMSE, 17 rue Sully, BP 86510, F-21065 Dijon cedex, France.
authors.forename: D
authors.initials: D
authors.lastname: Van Tuinen
mesh_terms: D017931:DNA Primers; D004275:DNA, Ribosomal / Q000737:chemistry; D015723:Gene Library; D014644:Genetic Variation; D005838:Genotype; D029902:Medicago / Q000382:microbiology*; D038821:Mycorrhizae / Q000145:classification* / Q000235:genetics / Q000502:physiology; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D013045:Species Specificity