title: Post-translational modification of crystallins in vitreous body from experimental autoimmune uveitis of rats.
abstract: Experimental autoimmune uveitis (EAU) is a well-known animal model of posterior uveitis that is one of the major causes of blindness. EAU could be induced in susceptible animals (i.e., Lewis rat) by immune reactions using evolutionarily conserved retinal proteins, such as interphoto-receptor retinoid binding protein (IRBP), or epitaphs of the protein. First, we prepared the following four test groups that subsequently increased or decreased inflammation. (1) Normal control group, (2) IRBP-induced uveitis group, (3) Hemin-treated uveitis group, and (4) Sn(IV) protoporphyrin IX dichloride (SnPP)-treated uveitis group. Second, in the vitreous bodies of Lewis rats, the infiltrated proteins were analyzed using two-dimensional electrophoresis (2-DE), MALDI-TOF/MS, and Micro LC/LC-MS/MS analysis. Finally, Western blotting was applied to confirm the relative amount of crystallins and phosphorylation sites of alphaB-crystallin. Thirty spots were identified in vitreous bodies, and 27 of these spots were members of the crystallin family. Unlike betaA4- and B2-crystallins (that were significantly increased without truncation), alphaA- and B-crystallins were only truncated in EAU vitreous body. Taken as a whole, in the rat EAU model, we suggest that post-translational truncations of alphaA- and alphaB-crystallins, phosphorylation of alphaB-crystallin, and new production of betaA4- and betaB2-crystallins are intercorrelated with uveitis progression and inflammatory responses.
authors.affiliation: Genome Research Center for Immune Disorders, School of Medicine, Wonkwang University, Iksan, Republic of Korea.
authors.forename: Song-Chul
authors.initials: SC
authors.lastname: Bahk
authors.forename: Jung-Un
authors.initials: JU
authors.lastname: Jang
authors.forename: Chang-Uk
authors.initials: CU
authors.lastname: Choi
authors.forename: Sook-Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Zee-Yong
authors.initials: ZY
authors.lastname: Park
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Yang
authors.forename: Jae-Duck
authors.initials: JD
authors.lastname: Kim
authors.forename: Yun-Sik
authors.initials: YS
authors.lastname: Yang
authors.forename: Hun-Taeg
authors.initials: HT
authors.lastname: Chung
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000378:metabolism*; D002853:Chromatography, Liquid; D003459:Crystallins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006427:Hemin / Q000494:pharmacology; D008297:Male; D008665:Metalloporphyrins / Q000494:pharmacology; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D011524:Protoporphyrins / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D015866:Uveitis, Posterior / Q000378:metabolism*; D014822:Vitreous Body / Q000378:metabolism*; D038202:alpha-Crystallin A Chain / Q000378:metabolism; D038203:alpha-Crystallin B Chain / Q000378:metabolism