title: Expression and role of EGFR ligands induced in airway cells by PM2.5 and its components.
abstract: The aim of the current study was to establish the epidermal growth factor receptor (EGFR) ligand expression profile in human airway epithelial cells exposed to either particulate matter (PM) with an aerodynamic diameter <2.5 microm (PM(2.5)) or its components and the involvement of EGFR ligands in PM(2.5)-provoked airway inflammation. EGFR ligand mRNA and protein expression were studied in a human bronchial epithelial cell line and normal nasal cells exposed to noncytotoxic concentrations of PM(2.5) or its components. The autocrine role of EGFR ligands in airway epithelial cell pro-inflammation was determined by adding conditioned media from PM(2.5)-treated cells to fresh cells and measuring the secretion of granulocyte-macrophage colony-stimulating factor (GM-CSF), a pro-inflammatory biomarker. PM(2.5)increased amphiregulin, transforming growth factor-alpha and heparin-binding EGF-like growth factor mRNA expression and protein secretion, with a slight contribution of aqueous metallic compounds and a strong participation of organic components putatively attributed to PM polyaromatic hydrocarbon content. PM(2.5)-induced EGFR ligands were involved in cellular GM-CSF release. The current study revealed upregulation of several epidermal growth factor receptor ligands by airway epithelial cells exposed to particulate matter with an aerodynamic diameter <2.5 microm and their contribution to bronchial epithelial cell granulocyte-macrophage colony-stimulating factor secretion by an autocrine action, suggesting that these ligands could elicit and sustain the particulate matter-induced airway pro-inflammatory response and contribute to bronchial remodelling.
authors.affiliation: Laboratory of Cytophysiology and Cellular Toxicology, Tour 53-54, Case Courrier 7073, University of Paris Diderot-Paris 7, 2 place Jussieu, 75 251 Paris Cedex 05, France. melina.rumelhard@univ-diderot-paris.fr
authors.forename: M
authors.initials: M
authors.lastname: Rumelhard
authors.forename: K
authors.initials: K
authors.lastname: Ramgolam
authors.forename: R
authors.initials: R
authors.lastname: Hamel
authors.forename: F
authors.initials: F
authors.lastname: Marano
authors.forename: A
authors.initials: A
authors.lastname: Baeza-Squiban
mesh_terms: D019898:Autocrine Communication / Q000187:drug effects; D002460:Cell Line; D017077:Culture Media, Conditioned; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008024:Ligands; D008670:Metals / Q000032:analysis; D010316:Particle Size; D052638:Particulate Matter / Q000494:pharmacology*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism; D012137:Respiratory System / Q000166:cytology* / Q000187:drug effects*