Induction of heat shock protein 70 by sodium arsenite attenuates burn-induced intestinal injury in severe burned rats.
The present study was designed to assess the effects of induced heat shock protein 70 (HSP70) on intestinal injury after severe burn. Wistar rats were randomly divided into four groups: control group, burn group (B group), sodium arsenite pretreatment group (SA group), and sodium arsenite+quercetin pretreatment group (SA+Qu group). Plasma endotoxin and d-lactic acid content were determined at 3, 6, 12, 24, and 48h after severe burn. Samples of small intestine were obtained for histologic assessment of intestinal mucosal injury and the expression of HSP70 was assayed by Western blot. Apoptosis of the intestinal epithelial cells was examined by the TUNEL method. Results showed that SA pretreatment significantly increased expression of HSP70 in the small intestine. SA pretreatment attenuated the burn-induced increase in plasma endotoxin and d-lactic acid content, intestinal injury scores and the percentage of apoptotic intestinal epithelial cells. Co-administration of quercetin with SA abolished the SA-induced HSP70 over-expression and the beneficial effects of SA. Our findings suggest increasing expression of HSP70 induced by SA pretreatment attenuates burn-induced intestinal injury apparently by preventing apoptosis.
Yuan
Zhi-Qiang
ZQ
Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400038, People's Republic of China.
Peng
Yi-Zhi
YZ
Li
Xiao-Lu
XL
Huang
Yue-Sheng
YS
Yang
Zong-Cheng
ZC
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D018053:Arsenites / Q000494:pharmacology*; D015153:Blotting, Western; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D004731:Endotoxins / Q000097:blood; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D020287:In Situ Nick-End Labeling; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019344:Lactic Acid / Q000097:blood; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D017670:Sodium Compounds / Q000494:pharmacology*