Evaluation of current methods for the measurement of serum anti double-stranded DNA antibodies.
Autoantibodies to double-stranded DNA (dsDNA) are, by definition, serological markers of systemic lupus erythematosus. However, the clinical value of anti-dsDNA antibodies largely depends on the assay principle and analytical variables of the methods used to quantitate and immunologically characterize them. In the present article, an overview of current methods for anti-dsDNA antibody detection is presented, together with a look at the future trends in technologies newly employed in this field.
Ghirardello
Anna
A
Division of Rheumatology, University of Padova, Italy.
Villalta
Danilo
D
Morozzi
Gabriella
G
Afeltra
Antonella
A
Galeazzi
Mauro
M
Gerli
Roberto
R
Mathieu
Alessandro
A
Meroni
Pier Luigi
PL
Migliorini
Paola
P
Radice
Antonella
A
Riccieri
Valeria
V
Ruffatti
Amelia
A
Sebastiani
Gian Domenico
GD
Tincani
Angela
A
Doria
Andrea
A
D000818:Animals; D000974:Antibodies, Antinuclear / Q000097:blood* / Q000276:immunology; D004247:DNA / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000145:classification / Q000379:methods*; D046228:Microarray Analysis / Q000379:methods*; D012680:Sensitivity and Specificity