Triglyceride sub-classes of various dog adipose tissue sites.
Lipids were extracted from four dog adipose tissue sites, perirenal, pericardial, inguinal subcutaneous, and mesenteric. The triglycerides were purified by thin-layer chromatography and the sub-classes separated by silver nitrate-silica gel plates. Gas chromatography was used to delineate the fatty acid composition of each subclass. The major sub-class was 011, followed by 012, 001, 111 and 112 in that order (0=saturated fatty acid; 1=one double bond; 2=two double bonds). The other components were present in minor amounts. The pericardial triglyceride had more 012 while the perirenal adipose tissue contained less 011 and more 001 than other sites. The other sub-classes were essentially similar.The distribution, based on 100%, of each saturated fatty acid was not the same in all sub-classes, although the over-all average was similar in each site.
Gold
M
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Department of Physiology, Hahnemann Medical College, 19102, Philadelphia, Pennsylvania.