title: Accuracy of Burkholderia pseudomallei identification using the API 20NE system and a latex agglutination test.
abstract: In an evaluation of the API 20NE for the identification of Burkholderia spp., 792/800 (99%) Burkholderia pseudomallei and 17/19 (89%) B. cepacia isolates were correctly identified but 10 B. mallei and 98 B. thailandensis isolates were not correctly identified. A latex agglutination test was positive for 796/800 (99.5%) B. pseudomallei isolates and negative for 120 other oxidase-positive gram-negative bacilli.
authors.affiliation: Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Premjit
authors.initials: P
authors.lastname: Amornchai
authors.forename: Wirongrong
authors.initials: W
authors.lastname: Chierakul
authors.forename: Vanaporn
authors.initials: V
authors.lastname: Wuthiekanun
authors.forename: Yuvadee
authors.initials: Y
authors.lastname: Mahakhunkijcharoen
authors.forename: Rattanaphone
authors.initials: R
authors.lastname: Phetsouvanh
authors.forename: Bart J
authors.initials: BJ
authors.lastname: Currie
authors.forename: Paul N
authors.initials: PN
authors.lastname: Newton
authors.forename: Nguyen
authors.initials: N
authors.lastname: van Vinh Chau
authors.forename: Surasakdi
authors.initials: S
authors.lastname: Wongratanacheewin
authors.forename: Nicholas P J
authors.initials: NP
authors.lastname: Day
authors.forename: Sharon J
authors.initials: SJ
authors.lastname: Peacock
mesh_terms: D000818:Animals; D016957:Burkholderia pseudomallei / Q000302:isolation & purification*; D003951:Diagnostic Errors; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D011933:Reagent Kits, Diagnostic