title: Comparison of column chromatographic methods for the quantitative determination of mono- and digalactodiglycerides in fresh alfalfa (Medicago sativa).
abstract: Three column chromatographic procedures for separating and recovering the galactolipids in fresh alfalfa extracts were compared. Silicic acid chromatography yielded pure fractions by thin-layer chromatography, infrared absorption, and chemical analysis. The carbon-Celite column gave the highest yield of monogalactodiglyceride. Of the 1.2% total lipids of fresh alfalfa, approximately 12% was monogalactodiglyceride and 8% was digalactodiglyceride. Linolenic acid accounted for about 90% of the total fatty acids in these components.
authors.affiliation: Institute of Marine Resources, Department of Nutritional Sciences, University of California, Berkeley, California.
authors.forename: J
authors.initials: J
authors.lastname: van der Veen
authors.forename: K
authors.initials: K
authors.lastname: Hirota
authors.forename: H S
authors.initials: HS
authors.lastname: Olcott