title: Electrokinetic separation of chiral compounds.
abstract: Femtomole amounts of racemic mixtures of derivatized amino acids were resolved and analyzed rapidly in about 10 minutes by means of high-voltage zone electrophoresis with laser-fluorescence detection. The electrophoresis was performed in capillary columns containing a chiral support electrolyte. A number of dansyl amino acids were resolved by the diastereomeric interaction between the DL-amino acid and the copper(II) complex of L-histidine present in the support electrolyte. A combination of electro-osmotic and electrophoretic action caused all species, positively charged, neutral, and negatively charged, to pass through the 0.5- nanoliter detection volume where they were subjected to laser excitation.
authors.forename: E
authors.initials: E
authors.lastname: Gassmann
authors.forename: J E
authors.initials: JE
authors.lastname: Kuo
authors.forename: R N
authors.initials: RN
authors.lastname: Zare