title: Wilms' tumor 1 message and protein expression in bone marrow failure syndrome and acute leukemia.
abstract: Wilms' tumor 1 (WT1) is a useful marker for the diagnosis of acute leukemia and myelodysplastic syndromes (MDS). In the current study quantitative reverse transcription-polymerase chain reaction and immunostaining were used simultaneously to examine the relationship between WT1 RNA and protein level and also to evaluate WT1 as a tool to differentiate aplastic anemia (AA) and MDS refractory anemia (RA). Three types of WT1 messages (total, exon 5(+) and KTS(+)) and WT1 immunostaining of these diseases were analyzed. An increase of all three WT1 messages in high-grade MDS and acute leukemia was observed as compared with the normal control, whereas there was no significant difference in WT1 message between AA and RA, suggesting that WT1 message is not a good tool to discriminate AA and RA. No significant difference was observed between normal and RA, except for exon 5 message. Three WT1 message levels had a significant correlation, suggesting that the total WT1 message is sufficient for clinical practice. Positive immunostaining of WT1 was observed only in the portion of acute leukemia and overt leukemia (OL) transformed from MDS with a high WT1 message level, suggesting the relatively high detection threshold of WT1 protein with the immunostaining method.
authors.affiliation: Department of Laboratory Medicine, Nagoya University School of Medicine, Nagoya, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Iwasaki
authors.forename: Chiho
authors.initials: C
authors.lastname: Sugisaki
authors.forename: Kohzo
authors.initials: K
authors.lastname: Nagata
authors.forename: Kenji
authors.initials: K
authors.lastname: Takagi
authors.forename: Akira
authors.initials: A
authors.lastname: Takagi
authors.forename: Tetsuhito
authors.initials: T
authors.lastname: Kojima
authors.forename: Masafumi
authors.initials: M
authors.lastname: Ito
authors.forename: Shigeo
authors.initials: S
authors.lastname: Nakamura
authors.forename: Tomoki
authors.initials: T
authors.lastname: Naoe
authors.forename: Takashi
authors.initials: T
authors.lastname: Murate
mesh_terms: D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000754:Anemia, Refractory, with Excess of Blasts / Q000235:genetics / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D003937:Diagnosis, Differential; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007124:Immunoenzyme Techniques; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000032:analysis; D025721:WT1 Proteins / Q000235:genetics / Q000378:metabolism