Nosocomial infection caused by class 1 integron-carrying Staphylococcus aureus in a hospital in South China.
Staphylococcus aureus isolates (n = 30) from the environment or from surgical patients in a hospital in Guangzhou, China were investigated for the presence of class 1 integrons. The class 1 integrase (intI1) gene and its 3' conserved segment were detected by PCR. IntI1-positive isolates were further analysed for the presence of resistance gene cassettes using specific primers, intI1-K and In-B. All isolates were also subjected to multilocus sequence typing (MLST) and random amplified polymorphic DNA (RAPD)-PCR analysis. Sixteen (53%) clinical and environmental isolates were positive for the class 1 integrase gene and were also found to possess the aadA2 gene. The 30 isolates were classified into six distinct genotypes by RAPD-PCR analysis: type A (n = 2); type B (n = 2); type C (n = 3); type D (n = 7); type E (n = 8); and type F (n = 8). All isolates belonged to the same sequence type (ST239) by MLST. These results indicated transmission of S. aureus between the environment and patients, as well as the probability of nosocomial infection.
Xu
Z
Z
School of Biological Science and Bioengineering, South China University of Technology, Guangzhou, China.
Shi
L
L
Zhang
C
C
Zhang
L
L
Li
X
X
Cao
Y
Y
Li
L
L
Yamasaki
S
S
D002681:China / Q000453:epidemiology; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology; D013502:General Surgery; D006757:Hospital Units; D006801:Humans; D032023:Integrons / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*