Enrichment of a population of mammary gland cells that form mammospheres and have in vivo repopulating activity.
The identification of mammary gland stem cells (MGSC) or progenitors is important for the study of normal breast development and tumorigenesis. Based on their immunophenotype, we have isolated a population of mouse mammary gland cells that are capable of forming "mammospheres" in vitro. Importantly, mammospheres are enriched for cells that regenerate an entire mammary gland on implantation into a mammary fat pad. We also undertook cytogenetic analyses of mammosphere-forming cells after prolonged culture, which provided preliminary insight into the genomic stability of these cells. Our identification of new cell surface markers for enriching mammosphere-initiating cells, including endoglin and prion protein, will facilitate the elucidation of the cell biology of MGSC.
Liao
Mai-Jing
MJ
Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA.
Zhang
Cheng Cheng
CC
Zhou
Beiyan
B
Zimonjic
Drazen B
DB
Mani
Sendurai A
SA
Kaba
Megan
M
Gifford
Ann
A
Reinhardt
Ferenc
F
Popescu
Nicholas C
NC
Guo
Wenjun
W
Eaton
Elinor Ng
EN
Lodish
Harvey F
HF
Weinberg
Robert A
RA
D053687:Adult Stem Cells / Q000166:cytology* / Q000637:transplantation*; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D048430:Cell Shape; D005260:Female; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000637:transplantation*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009940:Organoids / Q000166:cytology