title: Acquired AmpC type beta-lactamases: an emerging problem in Italian long-term care and rehabilitation facilities.
abstract: We report the multiple detection of Proteus mirabilis isolates, from 4 different long-term care and rehabilitation facilities (LTCRFs) of Northern Italy, resistant to expanded-spectrum cephalosporins and cephamycins and producing an acquired ampC-like beta-lactamase, named CMY-16. Genotyping by PFGE showed that isolates were clonally related to each other, although not identical. In all isolates the bla(CMY16) gene was not transferable by conjugation and was found to be carried on the chromosome. These results revealed multifocal spreading of a CMY-16 producing P. mirabilis clone in Northern Italy and emphasize the emergence of similar acquired resistance determinants in the LTCRFs setting.
authors.affiliation: Dipartimento di Scienze Morfologiche Eidologiche e Cliniche, sezione di Microbiologia, Università di Pavia, Pavia, Italy. r.miglia@unipv.it
authors.forename: Roberta
authors.initials: R
authors.lastname: Migliavacca
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Nucleo
authors.forename: Marco M
authors.initials: MM
authors.lastname: D'Andrea
authors.forename: Melissa
authors.initials: M
authors.lastname: Spalla
authors.forename: Tommaso
authors.initials: T
authors.lastname: Giani
authors.forename: Laura
authors.initials: L
authors.lastname: Pagani
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D002511:Cephalosporins / Q000494:pharmacology; D002513:Cephamycins / Q000494:pharmacology; D002876:Chromosomes, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D007558:Italy; D008134:Long-Term Care; D011512:Proteus Infections / Q000382:microbiology*; D011513:Proteus mirabilis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D012047:Rehabilitation Centers; D013045:Species Specificity; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*