Negative staining across holes: application to fibril and tubular structures.
The negative staining technique, when used with holey carbon support films, presents superior imaging conditions than is the case when samples are adsorbed to continuous carbon films. A demonstration of this negative staining approach is presented, using ammonium molybdate in combination with trehalose, applied to several fibrillar and tubular samples. Fibrils formed from the amyloid-beta peptide and the protease inhibitor pepstain A spread very well unsupported across holes and the different polymorphic fibril forms can be readily assessed. However, tubular forms of amyloid-beta have a tendency to be flattened, due to surface tension forces prior to and during specimen drying. Sub-fibril assembly forms and D-banded rat tail type 1 collagen fibres are presented. The air-dried collagen images produced are shown to contain almost as much detail as those obtainable by cryo-negative staining. Fragile DNA and DNA-protein nanotubes are also shown to yield superior quality images to those produced on continuous carbon films. The iron-storage protein, frataxin, creates elongated oligomeric assemblies, containing bound ferrihydrite microcrystals. The iron particles within these flexuous oligomers can be defined in the presence of ammonium molybdate, but they are more readily demonstrated if the frataxin is spread across holes in the presence of trehalose alone. The samples used here serve to show the likely benefit obtainable from negative staining across holes for a range of other fibrillar and tubular samples in biology, medicine and nanobiotechnology.
Harris
J Robin
JR
Institute of Zoology, University of Mainz, D-55099 Mainz, Germany. rharris@mail.uni-mainz.de
D000682:Amyloid / Q000737:chemistry / Q000648:ultrastructure; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000648:ultrastructure; D000818:Animals; D002244:Carbon; D003094:Collagen / Q000737:chemistry / Q000648:ultrastructure; D004247:DNA / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D033862:Iron-Binding Proteins / Q000737:chemistry / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008982:Molybdenum; D036103:Nanotechnology; D016624:Negative Staining / Q000379:methods*; D010436:Pepstatins / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D011506:Proteins / Q000737:chemistry / Q000648:ultrastructure; D051381:Rats; D014199:Trehalose; D000096923:Frataxin