Cytotoxicity of the novel spin trapping compound 5-ethoxycarbonyl-3,5-dimethyl-pyrroline N-oxide (3,5-EDPO) and its derivatives.
ESR spin trapping allows detection of superoxide radicals. Novel spin traps forming more stable superoxide adducts (t(1/2) ca. 12-55 min) were tested for their toxicity to cultured cells. The following toxicity ranking was obtained: 4,5-DPPO>4-BEMPO approximately 3-BEMPO>trans-3,5-EDPO>3,5-DPPO approximately 4,5-DiPPO approximately 4,5-EDPO>cis-3,5-EDPO approximately 3,5-DiPPO>DEPMPO. In conclusion, 4,5-EDPO, cis-3,5-EDPO and 3,5-DiPPO can be recommended for further investigation of superoxide in biological systems.
Rohr-Udilova
Nataliya
N
Division of Gastroenterology and Hepatology, Department of Internal Medicine III, Medical University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria. rohr_udilova@yahoo.de
Stolze
Klaus
K
Sagmeister
Sandra
S
Parzefall
Wolfram
W
Marian
Brigitte
B
Nohl
Hans
H
Schulte-Hermann
Rolf
R
Grasl-Kraupp
Bettina
B
D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003497:Cyclic N-Oxides / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006836:Hydro-Lyases / Q000378:metabolism; D007536:Isomerism; D015394:Molecular Structure; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D010087:Oxides / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity*; D051381:Rats; D019097:Spin Trapping; D013329:Structure-Activity Relationship; D013481:Superoxides / Q000378:metabolism