Global proteomic profiling of native outer membrane vesicles derived from Escherichia coli.
Gram-negative bacteria constitutively secrete native outer membrane vesicles (OMVs) into the extracellular milieu. Although recent progress in this area has revealed that OMVs are essential for bacterial survival and pathogenesis, the mechanism of vesicle formation and the biological roles of OMVs have not been clearly defined. Using a proteomics approach, we identified 141 protein components of Escherichia coli-derived native OMVs with high confidence; two separate analyses yielded identifications of 104 and 117 proteins, respectively, with 80 proteins overlapping between the two trials. In the group of identified proteins, the outer membrane proteins were highly enriched, whereas inner membrane proteins were lacking, suggesting that a specific sorting mechanism for vesicular proteins exists. We also identified proteins involved in vesicle formation, the removal of toxic compounds and attacking phage, and the elimination of competing organisms, as well as those involved in facilitating the transfer of genetic material and protein to other bacteria, targeting host cells, and modulating host immune responses. This study provides a global view of native bacterial OMVs. This information will help us not only to elucidate the biogenesis and functions of OMV from nonpathogenic and pathogenic bacteria but also to develop vaccines and antibiotics effective against pathogenic strains.
Lee
Eun-Young
EY
Department of Life Science and Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
Bang
Joo Young
JY
Park
Gun Wook
GW
Choi
Dong-Sic
DS
Kang
Ji Seoun
JS
Kim
Hyun-Jung
HJ
Park
Kyong-Su
KS
Lee
Jeong-Ok
JO
Kim
Yoon-Keun
YK
Kwon
Kyung-Hoon
KH
Kim
Kwang-Pyo
KP
Gho
Yong Song
YS
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification / Q000648:ultrastructure; D021961:Cell Membrane Structures / Q000737:chemistry* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D020869:Gene Expression Profiling; D013058:Mass Spectrometry; D008954:Models, Biological; D036103:Nanotechnology / Q000379:methods; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry