Size and shape separation of gold nanoparticles with preparative gel electrophoresis.
We employed agarose gel preparative electrophoresis to separate gold nanoparticles based on size, shape, and charge. The separating technique was first demonstrated by size separation of 5 nm, 15 nm, and 20 nm spherical gold nanoclusters; and further evidenced through the purification of crude 15 +/- 2.7 nm nanoclusters to nanoclusters that were 15 +/- 0.4 nm. The ability to separate gold nanoparticles by shape was also shown by the purification of a mixture of gold spheres, plates, and long rods.
Xu
Xiaoyou
X
Department of Chemistry and Biochemistry and Nanocenter, University of South Carolina, 631 Sumter Street, Columbia, SC 29208, USA.
Caswell
K Kenneth
KK
Tucker
Elizabeth
E
Kabisatpathy
Saswat
S
Brodhacker
K Lisa
KL
Scrivens
Walter A
WA
D004587:Electrophoresis, Agar Gel / Q000379:methods*; D006046:Gold / Q000032:analysis*; D017979:Gold Colloid / Q000032:analysis*; D007202:Indicators and Reagents; D053768:Metal Nanoparticles / Q000032:analysis* / Q000648:ultrastructure*; D046529:Microscopy, Electron, Transmission / Q000379:methods; D013194:Staining and Labeling