title: Dominance of Mycoplasma in the guts of the Long-Jawed Mudsucker, Gillichthys mirabilis, from five California salt marshes.
abstract: We analysed the gut microflora of the Long-Jawed Mudsucker, Gillichthys mirabilis by polymerase chain reaction/denaturing gradient gel electrophoresis and cloning and sequencing 16S rRNA gene amplicons. Fish were collected at five sites in northern and southern California, USA. The gut microflora assemblages of all G. mirabilis were similar, very simple and dominated by one or more Mycoplasma ribotypes. Hindguts were dominated by Mycoplasmas that were most similar to a ribotype retrieved from Atlantic salmon guts. A Mycoplasma ribotype that was 95% similar to Mycoplasma mobile was the dominant in the foregut.
authors.affiliation: Department of Marine Sciences, University of Georgia, Athens, GA 30602-3636, USA.
authors.forename: Nasreen
authors.initials: N
authors.lastname: Bano
authors.forename: Angelique
authors.initials: A
authors.lastname: DeRae Smith
authors.forename: William
authors.initials: W
authors.lastname: Bennett
authors.forename: Lydia
authors.initials: L
authors.lastname: Vasquez
authors.forename: James T
authors.initials: JT
authors.lastname: Hollibaugh
mesh_terms: D000818:Animals; D001483:Base Sequence; D002140:California; D017753:Ecosystem; D041981:Gastrointestinal Tract / Q000382:microbiology*; D009174:Mycoplasma / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D010473:Perciformes / Q000382:microbiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D053833:Wetlands