Simultaneous saccharification and fermentation of steam-pretreated bagasse using Saccharomyces cerevisiae TMB3400 and Pichia stipitis CBS6054.
Sugarcane bagasse--a residue from sugar and ethanol production from sugar cane--is a potential raw material for lignocellulosic ethanol production. This material is high in xylan content. A prerequisite for bioethanol production from bagasse is therefore that xylose is efficiently fermented to ethanol. In the current study, ethanolic fermentation of steam-pretreated sugarcane bagasse was assessed in a simultaneous saccharification and fermentation (SSF) set-up using either Saccharomyces cerevisiae TMB3400, a recombinant xylose utilizing yeast strain, or Pichia stipitis CBS6054, a naturally xylose utilizing yeast strain. Commercial cellulolytic enzymes were used and the content of water insoluble solids (WIS) was 5% or 7.5%. S. cerevisiae TMB3400 consumed all glucose and large fraction of the xylose in SSF. Almost complete xylose conversion could be achieved at 5% WIS and 32 degrees C. Fermentation did not occur with P. stipitis CBS6054 at pH 5.0. However, at pH 6.0, complete glucose conversion and high xylose conversion (>70%) was obtained. Microaeration was required for P. stipitis CBS6054. This was not necessary for S. cerevisiae TMB3400.
Rudolf
Andreas
A
Department of Chemical Engineering, Lund University, Box 124, SE-221 00 Lund, Sweden. andreas.rudolf@risoe.dk
Baudel
Henrique
H
Zacchi
Guido
G
Hahn-Hägerdal
Bärbel
B
Lidén
Gunnar
G
D002482:Cellulose / Q000378:metabolism*; D018920:Coculture Techniques; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D010843:Pichia / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D031786:Saccharum / Q000737:chemistry / Q000382:microbiology*; D013227:Steam; D014994:Xylose / Q000378:metabolism*