title: Hexameric ring structure of human MCM10 DNA replication factor.
abstract: The DNA replication factor minichromosome maintenance 10 (MCM10) is a conserved, abundant nuclear protein crucial for origin firing. During the transition from pre-replicative complexes to pre-initiation complexes, MCM10 recruitment to replication origins is required to provide a physical link between the MCM2-7 complex DNA helicase and DNA polymerases. Here, we report the molecular structure of human MCM10 as determined by electron microscopy and single-particle analysis. The MCM10 molecule is a ring-shaped hexamer with large central and smaller lateral channels and a system of inner chambers. This structure, together with biochemical data, suggests that this important protein uses its architecture to provide a docking module for assembly of the molecular machinery required for eukaryotic DNA replication.
authors.affiliation: Department of Pathology, University College London, London WC1E 6JJ, UK.
authors.forename: Andrei L
authors.initials: AL
authors.lastname: Okorokov
authors.forename: Alastair
authors.initials: A
authors.lastname: Waugh
authors.forename: Julie
authors.initials: J
authors.lastname: Hodgkinson
authors.forename: Andal
authors.initials: A
authors.lastname: Murthy
authors.forename: Hye Kyung
authors.initials: HK
authors.lastname: Hong
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Leo
authors.forename: Michael B
authors.initials: MB
authors.lastname: Sherman
authors.forename: Kai
authors.initials: K
authors.lastname: Stoeber
authors.forename: Elena V
authors.initials: EV
authors.lastname: Orlova
authors.forename: Gareth H
authors.initials: GH
authors.lastname: Williams
mesh_terms: D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004261:DNA Replication; D019281:Dimerization; D006801:Humans; D008854:Microscopy, Electron; D064110:Minichromosome Maintenance Proteins; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure