Cilia isolated from tetrahymena after membrane stabilization by 1,5-difluoro-2,4-dinitrobenzene.
The tendency for Tetrahymena pyriformis cells to lyse when they are centrifuged at speeds above 120g and then exposed to an ethanolic deciliating medium is inhibited by treatment with 1,5-difluoro-2,4-dinitrobenzene. This treatment facilitates greater yields and purer preparations of isolated cilia. Cilia from treated and untreated cells do not differ in some of their biochemical properties.
Culbertson
J R
JR