[Screening of polypeptides interacting with Rel homology domain of NF-kappaB p50 subunit].
OBJECTIVE
To obtain the polypeptides interacting with NF-kappaB p50 Rel homology domain (RHD) by yeast two-hybrid technique.


METHODS
Using NF-kappaB p50 RHD as bait, the polypeptides interacting with NF-kappaB p50 RHD were screened from a 16-peptide cDNA library by yeast two-hybrid technique. The false positive clones were screened out but the positive clone were identified by beta-GAL assay, yeast mating, and one to one yeast two-hybrid method.


RESULTS
8 positive clones were obtained. The gene sequences of the eight polypeptides were different from each other and their homologous proteins were not found in GenBank.


CONCLUSIONS
8 novel polypeptides interacting with NF-kappaB p50 RHD were obtained, but their functions need to be validated in further experiments.
Shen
Li-qun
LQ
Research Institute of Surgery, Daping Hospital, Chongqing 400042, China.
Xu
Xiang
X
Liang
Hua-ping
HP
Li
Sheng-mao
SM
D000595:Amino Acid Sequence; D030541:Databases, Genetic; D017930:Genes, Reporter; D007763:Lac Operon; D008969:Molecular Sequence Data; D052002:NF-kappa B p50 Subunit / Q000737:chemistry* / Q000378:metabolism*; D010455:Peptides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D010957:Plasmids / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D051996:Transcription Factor RelA / Q000378:metabolism*; D051841:Transcription Factor RelB / Q000378:metabolism*; D020798:Two-Hybrid System Techniques