title: Critical DNA binding interactions of the insulator protein CTCF: a small number of zinc fingers mediate strong binding, and a single finger-DNA interaction controls binding at imprinted loci.
abstract: The DNA-binding protein CTCF (CCCTC binding factor) mediates enhancer blocking insulation at sites throughout the genome and plays an important role in regulating allele-specific expression at the Igf2/H19 locus and at other imprinted loci. Evidence is also accumulating that CTCF is involved in large scale organization of genomic chromatin. Although CTCF has 11 zinc fingers, we show here that only 4 of these are essential to strong binding and that they recognize a core 12-bp DNA sequence common to most CTCF sites. By deleting individual fingers and mutating individual sites, we determined the orientation of binding. Furthermore, we were able to identify the specific finger and its point of DNA interaction that are responsible for the loss of CTCF binding when CpG residues are methylated in the imprinted Igf2/H19 locus. This single interaction appears to be critical for allele-specific binding and insulation by CTCF.
authors.affiliation: Dipartimento di Scienze Ambientali, Seconda Università degli Studi di Napoli via Vivaldi 43, 81100 Caserta, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Renda
authors.affiliation: Dipartimento di Scienze Ambientali, Seconda Università degli Studi di Napoli via Vivaldi 43, 81100 Caserta, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Baglivo
authors.affiliation: Laboratory of Molecular Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892-0540.
authors.forename: Bonnie
authors.initials: B
authors.lastname: Burgess-Beusse
authors.affiliation: Dipartimento di Scienze Ambientali, Seconda Università degli Studi di Napoli via Vivaldi 43, 81100 Caserta, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Esposito
authors.affiliation: Dipartimento di Scienze Ambientali, Seconda Università degli Studi di Napoli via Vivaldi 43, 81100 Caserta, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Fattorusso
authors.affiliation: Laboratory of Molecular Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892-0540. Electronic address: gary.felsenfeld@nih.gov.
authors.forename: Gary
authors.initials: G
authors.lastname: Felsenfeld
authors.affiliation: Dipartimento di Scienze Ambientali, Seconda Università degli Studi di Napoli via Vivaldi 43, 81100 Caserta, Italy. Electronic address: paolov.pedone@unina2.it.
authors.forename: Paolo V
authors.initials: PV
authors.lastname: Pedone
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D000076246:CCCTC-Binding Factor; D018899:CpG Islands; D004247:DNA / Q000737:chemistry*; D019175:DNA Methylation; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000502:physiology*; D004742:Enhancer Elements, Genetic; D018392:Genomic Imprinting; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000737:chemistry / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism; D016335:Zinc Fingers