Molecular cloning of an insertion sequence-like element from Vibrio anguillarum and its functional identification in E. coli.
The tdh (thermostable direct hemolysin) gene occurs in some strains of Vibrio species. All tdh genes are flanked by insertion sequence-like elements (ISV). All previous attempts have failed to detect transposition of these ISVs. In this work, we have built a transposition detection system in E. coli and succeeded in detecting the transposition of an insertion sequence-like element at a frequency of Km(r) mutants of 7.2 x 10(-6). A specific flanking sequence (5'-Py-Pu-3') was found on either side of the target duplication.
Liang
Na
N
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Wu
Haizhen
H
Ye
Jiang
J
Xu
Peng
P
Zhang
Yuanxing
Y
Zhang
Huizhan
H
D000595:Amino Acid Sequence; D001427:Bacterial Toxins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D006460:Hemolysin Proteins / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D014733:Vibrio / Q000235:genetics*