[Contribution of Western blotting to the diagnosis of hydatidosis].
The aim of this study is to evaluate the contribution of the immunoWesternblot for the diagnosis and the post surgical follow-up of the hydatidosis. 71 sera from patients with hydatidosis confirmed by surgery were studied. All had a negative hydatic serology by screening tests (enzyme-linked immunosorbent assay, hemagglutination, electrosyneresis). 12 patients with sera in pre and post operative were monitored for 2 years. The Echinococcus Western blot IgG permitted to rectify the diagnosis of hydatidosis in 67.6 %. The rate of positivity was 100 % for the multivesicular liver cysts, 60 % for the young cysts and 50 % for the calcified cysts. Western blot permitted to rectify the diagnosis of lung cysts in 62.5 % of cases and in 50 % of cranial-spinal localizations. Analysis of Western Blot evolution in the 12 patients followed in pre and post-surgical revealed the disappearance of the bands 16, 18 and 26-28kDa in 8 month in the 8 patients with complete exeresis. This study proved the value added of Western blot compared to the other traditional techniques for the immunodiagnostic and the post-surgical monitoring of hydatidosis.
Makni
F
F
Laboratoire de parasitologie-mycologie, CHU Habib-Bourguiba, Sfax, Tunisie.
Hachicha
L
L
Mseddi
F
F
Hammami
H
H
Cheikhrouhou
F
F
Sellami
H
H
Sellami
A
A
Mzali
R
R
Boujelbène
S
S
Rebaï
R
R
Beyrouti
I
I
Ayadi
A
A
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D015153:Blotting, Western; D002114:Calcinosis / Q000175:diagnosis / Q000469:parasitology; D020809:Central Nervous System Helminthiasis / Q000097:blood / Q000175:diagnosis; D018450:Disease Progression; D004443:Echinococcosis / Q000175:diagnosis* / Q000601:surgery; D004444:Echinococcosis, Hepatic / Q000097:blood / Q000175:diagnosis; D004445:Echinococcosis, Pulmonary / Q000097:blood / Q000175:diagnosis; D004446:Echinococcus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008970:Molecular Weight; D011184:Postoperative Period; D012680:Sensitivity and Specificity