title: Decreasing variability in your cell culture.
abstract: Culturing mammalian cells has not significantly changed in almost 50 years. Typically, a synthetic basal medium is chosen to meet the environmental and nutritional requirements of a given cell line. Components, such as amino acids, vitamins, inorganic salts, and a carbon source such as glucose are commonly found in the classical basal media formulation. These basal formulations normally will not support cell growth alone, but must be further supplemented with animal serum, usually fetal bovine serum (FBS) at a concentration of 5-20%. Recent advances in serum-free and chemically defined media formulations have provided cell culturists with options. When considering FDA regulations and potential risks to human health when manufacturing biologics or considering cell therapies, eliminating serum is of paramount concern. For a large majority of researchers however, using classical media with serum builds on previous generations of research and makes cell culture easier to perform.
authors.affiliation: GIBCO Cell Culture Systems, Invitrogen. aaron.stein@invitrogen.com
authors.forename: Aaron
authors.initials: A
authors.lastname: Stein
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured / Q000166:cytology* / Q000502:physiology*; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans