Chitosan-based hyaluronan hybrid polymer fibre scaffold for ligament and tendon tissue engineering.
To establish medical use of tissue engineering technology for ligament and tendon injuries, a scaffold was developed which has sufficient ability for cell growth, cell differentiation, and mechanical properties. The scaffold made from chitosan and 0.1 per cent hyaluronic acid has adequate biodegradability and biocompatibility. An animal experiment showed that the scaffold has less toxicity and less inflammation induction. Furthermore, in-vivo animal experiments showed that the mechanical properties of the engineered ligament or tendon had the possibility to stabilize the joint. It was shown that newly developed hybrid-polymer fibre scaffold has feasibility for joint tissue engineering.
Majima
T
T
Department of Orthopaedic Surgery, Hokkaido University School of Medicine, Sapporo, 060-8638, Japan. tkmajima@med.hokudai.ac.jp
Irie
T
T
Sawaguchi
N
N
Funakoshi
T
T
Iwasaki
N
N
Harada
K
K
Minami
A
A
Nishimura
S-I
SI
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D004548:Elasticity; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D048091:Guided Tissue Regeneration / Q000295:instrumentation* / Q000379:methods; D006820:Hyaluronic Acid / Q000737:chemistry*; D008022:Ligaments / Q000166:cytology / Q000254:growth & development*; D008422:Materials Testing; D011817:Rabbits; D013710:Tendons / Q000166:cytology / Q000254:growth & development*; D013718:Tensile Strength; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods