[Inhibitory effect of siRNA on heparanase expression and invasion ability of gastric cancer cells: an in vitro experiment].
OBJECTIVE
To investigate the inhibitory effect of siRNA on heparanase expression and invasion ability gastric cancer cells.


METHODS
A heparanase mRNA-targeting double-stranded siRNA was designed with the bioinformatics technology. Human gastric cancer cells of the line SGC7901 were cultured and transfected with the siRNA of the concentrations of 5, 10, 20, and 40 nmol/L respectively. Forty-eight hours later RT-PCR and Western blotting were applied to detect the mRNA and protein expression of heparanase. Millicell chamber assay was performed to detect the invasion ability of the SGC7901 cells. Blank control group and negative control group were set.


RESULTS
The mRNA expression level of the cells transfected with the siRNA of the concentrations 20 nmol/L and 40 nmol/L were 0.207 +/- 0.095 and 0.200 +/- 0.085 respectively, both significantly lower than that of the control group (0.60 +/- 0.09, both P < 0.05). Western blotting showed that the protein expression of heparanase of the different siRNA subgroups were all decreased dose-dependently; and no heparanase band was seen in the 40 nmol/L subgroup. The invasion rate of the siRNA group was significantly lower than that of the control group with a mean inhibition rate of (61 +/- 36)%.


CONCLUSIONS
RNAi inhibits the expression of heparanase and the invasion ability of human gastric cancer cells. Heparanase may be a new target in treatment of gastric cancer's metastasis.
Zhang
Yong
Y
Department of Oncosurgery, No.1 Hospital of China Medical University, Shenyang 110001, China.
Wang
Zhen-ning
ZN
Zhang
Xue
X
Xu
Hui-mian
HM
Jiang
Li
L
Luo
Yang
Y
Xing
Li-li
LL
Xu
Mi-duo
MD
Li
Juan
J
D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D014162:Transfection; D000099175:Heparanase