Interaction studies of DNA binding of ruthenium(II) mixed-ligand complexes: [Ru(phen)2(dtmi)]2+ and [Ru(phen)2(dtni)]2+.
Two new ligands, 3-(pyrazin-2-yl)-as-triazino[5,6-f]-5-methoxylisatin (dtmi), 3-(pyrazin-2-yl)-as-triazino[5,6-f]-5-nitroisatin (dtni) and their ruthenium(II) complexes [Ru(phen)2(dtmi)](ClO4)2 (1) and [Ru(phen)2(dtni)](ClO4)2 (2) have been prepared and characterized by elemental analysis, FAB-MS, ES-MS and 1H NMR. The DNA-binding behaviors of complexes have been studied by spectroscopic titration, viscosity measurements, thermal denaturation and circular dichromism (CD). The results indicate that the complexes 1 and 2 interact with calf thymus DNA (CT-DNA) by intercalative mode. The DNA-binding affinity of the complexes 2 is larger than that complex 1 does.
Liu
Yun-Jun
YJ
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China. lyjche@163.com
Wei
Xin Yu
XY
Wu
Fu-Hai
FH
Mei
Wen-Jie
WJ
He
Li-Xin
LX
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D006358:Hot Temperature; D007364:Intercalating Agents / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D008832:Microchemistry / Q000379:methods; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009691:Nucleic Acid Denaturation; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism; D010618:Phenanthrolines / Q000737:chemistry* / Q000378:metabolism; D011719:Pyrazines / Q000737:chemistry* / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D014783:Viscosity