Effects of honokiol and magnolol on acute and inflammatory pain models in mice.
The antinociceptive actions of honokiol and magnolol, two major bioactive constituents of the bark of Magnolia officinalis, were evaluated using tail-flick, hot-plate and formalin tests in mice. The effects of honokiol and magnolol on the formalin-induced c-Fos expression in the spinal cord dorsal horn as well as motor coordination and cognitive function were examined. Data showed that honokiol and magnolol did not produce analgesia in tail-flick, hot-plate paw-shaking and neurogenic phase of the overt nociception induced by intraplantar injection of formalin. However, honokiol and magnolol reduced the inflammatory phase of formalin-induced licking response. Consistently, honokiol and magnolol significantly decreased formalin-induced c-Fos protein expression in superficial (I-II) laminae of the L4-L5 lumbar dorsal horn. However, honokiol and magnolol did not elicit motor incoordination and memory dysfunction at doses higher than the analgesic dose. These results demonstrate that honokiol and magnolol effectively alleviate the formalin-induced inflammatory pain without motor and cognitive side effects, suggesting their therapeutic potential in the treatment of inflammatory pain.
Lin
Yi-Ruu
YR
Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan.
Chen
Hwei-Hsien
HH
Ko
Chien-Hsin
CH
Chan
Ming-Huan
MH
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D002492:Central Nervous System Depressants / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005404:Fixatives / Q000633:toxicity; D005557:Formaldehyde / Q000633:toxicity; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D017705:Lignans / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D008161:Lumbosacral Region; D031566:Magnolia / Q000737:chemistry; D008568:Memory / Q000187:drug effects; D051379:Mice; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D020671:Posterior Horn Cells / Q000378:metabolism / Q000473:pathology; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D000498:Allyl Compounds; D010636:Phenols