title: Autoantibody signature in human ductal pancreatic adenocarcinoma.
abstract: Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy characterized by rapid progression, invasiveness, and resistance to treatment. It is the fourth leading cause of cancer death with a 2% 5-year survival rate. Biomarkers for its early detection are lacking. This study was designed to use a proteomics-based approach as a means of identifying antigens that elicit a humoral response in PDAC patients. Antibodies against PDAC-associated antigens are useful for early cancer diagnosis and therapy. Proteins from PDAC cell lines were separated by 2-DE, and the serum IgG reactivity of 70 PDAC patients, 40 healthy subjects (HS), 30 non-PDAC tumor patients, and 15 chronic pancreatitis (CP) patients was tested by Western blot analysis. Spots specifically recognized by PDAC sera and revealed by mass spectrometry corresponded to metabolic enzymes or cytoskeletal proteins. Most were up-regulated in PDAC tissues. Thus, it seems that metabolic enzymes and cytoskeletal proteins are specific targets of the humoral response during PDAC. The results of further studies of these serological-defined antigens could be of diagnostic and therapeutic significance in PDAC.
authors.affiliation: Center for Experimental Research and Medical Studies and University of Turin, San Giovanni Battista Hospital, Turin, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Tomaino
authors.forename: Paola
authors.initials: P
authors.lastname: Cappello
authors.forename: Michela
authors.initials: M
authors.lastname: Capello
authors.forename: Claudia
authors.initials: C
authors.lastname: Fredolini
authors.forename: Antonio
authors.initials: A
authors.lastname: Ponzetto
authors.forename: Anna
authors.initials: A
authors.lastname: Novarino
authors.forename: Libero
authors.initials: L
authors.lastname: Ciuffreda
authors.forename: Oscar
authors.initials: O
authors.lastname: Bertetto
authors.forename: Claudio
authors.initials: C
authors.lastname: De Angelis
authors.forename: Enzo
authors.initials: E
authors.lastname: Gaia
authors.forename: Paola
authors.initials: P
authors.lastname: Salacone
authors.forename: Michele
authors.initials: M
authors.lastname: Milella
authors.forename: Paola
authors.initials: P
authors.lastname: Nisticò
authors.forename: Massimo
authors.initials: M
authors.lastname: Alessio
authors.forename: Roberto
authors.initials: R
authors.lastname: Chiarle
authors.forename: Maria G
authors.initials: MG
authors.lastname: Giuffrida
authors.forename: Mirella
authors.initials: M
authors.lastname: Giovarelli
authors.forename: Francesco
authors.initials: F
authors.lastname: Novelli
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000917:Antibody Formation; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D021441:Carcinoma, Pancreatic Ductal / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000096:biosynthesis / Q000276:immunology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007150:Immunohistochemistry; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000276:immunology*; D010179:Pancreas / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D050500:Pancreatitis, Chronic / Q000201:enzymology / Q000276:immunology / Q000378:metabolism