Evaluation of an ovine testis cell line (OA3.Ts) for propagation of capripoxvirus isolates and development of an immunostaining technique for viral plaque visualization.
An ovine testis cell line (OA3.Ts) was evaluated and compared with primary lamb kidney (LK) cells for its utility in capripoxvirus propagation and titration. A comparison of OA3.Ts cell growth kinetics and morphology at low (<33) and high (34-36) passage levels indicated a difference in both characteristics. However, viral titers determined in low and high passage OA3.Ts cells were comparable with those obtained using LK cells. Capripoxvirus infection of OA3.Ts and LK cells resulted in a similar cytopathic effect, which allowed for the detection of discrete viral plaques following immunostaining with capripoxvirus-specific antiserum.
Babiuk
Shawn
S
National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg MB, R3E 3M4 Canada. babiuks@inspection.gc.ca
Parkyn
Geoff
G
Copps
John
J
Larence
June E
JE
Sabara
Marta I
MI
Bowden
Timothy R
TR
Boyle
David B
DB
Kitching
R Paul
RP
D000818:Animals; D018151:Capripoxvirus / Q000502:physiology*; D002460:Cell Line; D007668:Kidney / Q000166:cytology; D008297:Male; D012756:Sheep; D013194:Staining and Labeling; D013737:Testis / Q000166:cytology*; D010948:Viral Plaque Assay / Q000379:methods / Q000662:veterinary*; D014776:Virus Cultivation / Q000379:methods / Q000662:veterinary*