In vivo mapping of temporospatial changes in manganese enhancement in rat brain during epileptogenesis.
Mesial temporal lobe epilepsy is associated with structural and functional abnormalities, such as hippocampal sclerosis and axonal reorganization. The temporal evolution of these changes remains to be determined, and there is a need for in vivo imaging techniques that can uncover the epileptogenic processes at an early stage. Manganese-enhanced magnetic resonance imaging may be useful in this regard. The aim of this study was to analyze the temporospatial changes in manganese enhancement in rat brain during the development of epilepsy subsequent to systemic kainate application (10 mg/kg i.p.). MnCl(2) was given systemically on day 2 (early), day 15 (latent), and 11 weeks (chronic phase) after the initial status epilepticus. Twenty-four hours after MnCl(2) injection T1-weighted 3D MRI was performed followed by analysis of manganese enhancement. In the medial temporal lobes, there was a pronounced decrease in manganese enhancement in CA1, CA3, dentate gyrus, entorhinal cortex and lateral amygdala in the early phase. In the latent and chronic phases, recovery of the manganese enhancement was observed in all these structures except CA1. A significant increase in manganese enhancement was detected in the entorhinal cortex and the amygdala in the chronic phase. In the latter phase, the structurally intact cerebellum showed significantly decreased manganese enhancement. The highly differentiated changes in manganese enhancement are likely to represent the net outcome of a number of pathological and pathophysiological events, including cell loss and changes in neuronal activity. Our findings are not consistent with the idea that manganese enhancement primarily reflects changes in glial cells.
Alvestad
Silje
S
Department of Neuroscience, Norwegian University of Science and Technology (NTNU), N-7489 Trondheim, Norway.
Goa
Pål Erik
PE
Qu
Hong
H
Risa
Øystein
Ø
Brekken
Christian
C
Sonnewald
Ursula
U
Haraldseth
Olav
O
Hammer
Janniche
J
Ottersen
Ole Petter
OP
Håberg
Asta
A
D000818:Animals; D001921:Brain / Q000473:pathology*; D001931:Brain Mapping / Q000379:methods*; D003287:Contrast Media; D004827:Epilepsy / Q000175:diagnosis*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008345:Manganese; D051381:Rats; D017207:Rats, Sprague-Dawley