Solid-phase synthesis and thermal denaturation study of cyclic PNAs targeting the HIV-1 TAR RNA loop.
Cyclic PNAs targeting the HIV-1 TAR RNA loop have been synthesized following a convenient solid-phase strategy which allows on-resin cyclisation. UV-monitored thermal denaturation studies demonstrate that these cyclic PNAs are able to strongly interact with their TAR RNA target, very likely through the formation of a six-base pair stable complex, involving the TAR RNA loop.
Upert
Gregory
G
Laboratoire de Chimie des Molécules Bioactives et des Arômes (LCMBA), UMR UNSA-CNRS 6001, Université de Nice-Sophia Antipolis, 06108 Nice Cedex 2, France.
Mehiri
Mohamed
M
Di Giorgio
Audrey
A
Condom
Roger
R
Patino
Nadia
N
D015497:HIV-1 / Q000235:genetics*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D012367:RNA, Viral / Q000187:drug effects*; D013056:Spectrophotometry, Ultraviolet