Correlations between differences in amino-terminal sequences and different hemolytic activity of sticholysins.
Sticholysins I and II (St I and St II) are cytolysins produced by the sea anemone Stichodactyla helianthus. In spite of their 93% sequence homology, St II is more hemolytic against human erythrocytes than St I. In order to establish the possible causes of this difference, we studied the hemolytic activity of synthetic peptides containing sequences from the N-termini of both proteins. The results demonstrated that the differences in hemolytic activity of the toxins could be ascribed at least partly to differences in their N-termini.
Cilli
Eduardo M
EM
Department of Biochemistry and Technological Chemistry, Institute of Chemistry, UNESP--São Paulo State University, SP, Brazil.
Pigossi
Fábio T
FT
Crusca
Edson
E
Ros
Uris
U
Martinez
Diana
D
Lanio
Maria E
ME
Alvarez
Carlos
C
Schreier
Shirley
S
D000595:Amino Acid Sequence; D003064:Cnidarian Venoms / Q000737:chemistry* / Q000633:toxicity; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D008969:Molecular Sequence Data; D009930:Organic Chemicals / Q000737:chemistry / Q000633:toxicity; D013329:Structure-Activity Relationship