title: Comparative analysis of mutants in the mycothiol biosynthesis pathway in Mycobacterium smegmatis.
abstract: The role of mycothiol in mycobacteria was examined by comparative analysis of mutants disrupted in the four known genes encoding the protein machinery needed for mycothiol biosynthesis. These mutants were sensitive to acid stress, antibiotic stress, alkylating stress, and oxidative stress indicating that mycothiol and mycothiol-dependent enzymes protect the mycobacterial cell against attack from various different types of stresses and toxic agents.
authors.affiliation: Department of Biology, California State University-Fresno, Fresno, CA 937401, USA.
authors.forename: Mamta
authors.initials: M
authors.lastname: Rawat
authors.forename: Chantale
authors.initials: C
authors.lastname: Johnson
authors.forename: Vanessa
authors.initials: V
authors.lastname: Cadiz
authors.forename: Yossef
authors.initials: Y
authors.lastname: Av-Gay
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D003545:Cysteine / Q000096:biosynthesis*; D006020:Glycopeptides / Q000096:biosynthesis*; D007294:Inositol / Q000096:biosynthesis*; D009154:Mutation; D020102:Mycobacterium smegmatis / Q000235:genetics* / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*