title: Expression and subcellular localization of E-cadherin, alpha-catenin, and beta-catenin in 8 feline mammary tumor cell lines.
abstract: Protein expression and subcellular localization of E-cadherin, alpha-catenin, and beta-catenin in 8 feline mammary tumor cell lines were examined by western blot analysis and fluorescence immunocytochemistry. A low E-cadherin expression was observed in FNN-m cells. Furthermore, compared to other cell lines, two E-cadherin bands existed in FMC-p1 cells and were localized in the perinuclear region; distinct radial lines were observed in the cytoplasm. A low alpha-catenin expression was observed in FON-m cells, but there were no apparent abnormalities in its localization. In contrast, similar levels of beta-catenin expression and cytoplasmic localization were observed in all cell lines.
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, Tokyo, Japan.
authors.forename: Shun-roku
authors.initials: SR
authors.lastname: Takauji
authors.forename: Manabu
authors.initials: M
authors.lastname: Watanabe
authors.forename: Rina
authors.initials: R
authors.lastname: Uyama
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Nozomi
authors.initials: N
authors.lastname: Miyajima
authors.forename: Manabu
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Ryohei
authors.initials: R
authors.lastname: Nishimura
authors.forename: Sumio
authors.initials: S
authors.lastname: Sugano
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D002415:Cats; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D015674:Mammary Neoplasms, Animal / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D051178:alpha Catenin / Q000235:genetics* / Q000378:metabolism; D051176:beta Catenin / Q000235:genetics* / Q000378:metabolism