EMR1, the human homolog of F4/80, is an eosinophil-specific receptor.
The EGF-TM7 F4/80 is a defining marker of murine macrophage populations. Applying flow cytometric analysis using the newly generated mAb A10, and quantitative real-time PCR, we here report the surprising observation that the human ortholog of F4/80, EGF-like module containing mucin-like hormone receptor (EMR)1, is absent on mononuclear phagocytic cells including monocytes, macrophages, and myeloid dendritic cells. Unexpectedly, we found that EMR1 expression is restricted to eosinophilic granulocytes, where expression is overlapping with the eotaxin receptor CCR3 and the immunoglobulin-like lectin Siglec-8. Absence on other leukocytes, including basophils, implies that EMR1 is a highly specific marker for eosinophils in humans.
Hamann
Jörg
J
Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. j.hamann@amc.uva.nl
Koning
Nathalie
N
Pouwels
Walter
W
Ulfman
Laurien H
LH
van Eijk
Marco
M
Stacey
Martin
M
Lin
Hsi-Hsien
HH
Gordon
Siamon
S
Kwakkenbos
Mark J
MJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000943:Antigens, Differentiation / Q000737:chemistry / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D016466:CHO Cells; D002135:Calcium-Binding Proteins; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004804:Eosinophils / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D008253:Macaca mulatta; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009077:Mucins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002679:Pan troglodytes; D010215:Papio; D011148:Pongo pygmaeus; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012452:Saguinus; D040681:Structural Homology, Protein