title: Detection and genetic characterization of norovirus in oysters from China and Japan.
abstract: A total of 225 oysters from China and Japan were collected during October 2005 to September 2006 and were then tested for the presence of norovirus by RT-nested PCR. The detection rate of norovirus was different between China and Japan, accounting for 14.6% (19 of 130) and 25.3% (24 of 95), respectively. In China, norovirus in oyster was detected continuously from July to February with the highest prevalence in August, October and November (each of 21%, 4 of 19). On the other hand, norovirus in Japan was found year-round with highest prevalence in March and October (each of 20.8%, 5 of 24). Norovirus strains detected were subjected to further characterization by sequence analysis. It was found that the norovirus strains belonged to only two distinct genotypes, the GII/3 (known as the Mexico virus cluster) and the GII/4 (known as the Lordsdale virus cluster). In China, the norovirus GII/4 was the most predominant, accounting for 78.9% (15 of 19). In contrast, it was interesting that both the norovirus GII/4 and the norovirus GII/3 were co-predominant with a prevalence of 50% (12 of 24) in Japan. Another interesting feature of the study was that the norovirus GII/4 strains in oysters from both countries were grouped into two distinct variant clusters known as the Farmington Hills variant and the Hunter variant. More than 102 copies of norovirus were detected in 41 of 43 oysters. This study provided additional evidence of the presence of norovirus in oysters and is also the first report to demonstrate the existence of norovirus variants in oysters.
authors.affiliation: Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Tung Gia
authors.initials: TG
authors.lastname: Phan
authors.forename: Pattara
authors.initials: P
authors.lastname: Khamrin
authors.forename: Miho
authors.initials: M
authors.lastname: Akiyama
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Yagyu
authors.forename: Shoko
authors.initials: S
authors.lastname: Okitsu
authors.forename: Niwat
authors.initials: N
authors.lastname: Maneekarn
authors.forename: Osamu
authors.initials: O
authors.lastname: Nishio
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ushijima
mesh_terms: D000818:Animals; D002681:China; D016000:Cluster Analysis; D014644:Genetic Variation; D005838:Genotype; D007564:Japan; D029322:Norovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010125:Ostreidae / Q000737:chemistry / Q000821:virology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons