title: Analysis of inter-(simple sequence repeat) PCR products from human colorectal cancers.
abstract: Genomic instability is a fundamental characteristic of solid tumors, and understanding genomic instability should significantly clarify the process of tumorigenesis. We adapted the sampling technique of inter-(simple sequence repeat) PCR [inter-(SSR) PCR] to measure genetic alterations between simple sequence repeats in colorectal tumors. It becomes important to precisely define both normal and altered inter-(SSR) PCR products. BLAT searches of 131 cloned inter-(SSR) PCR sequences reveal that inter-(SSR) PCR products are located on almost all the chromosomes except chromosome Y, indicating that inter-(SSR) PCR samples a representative diverse range of the genome. We confirm that a change in the pattern of the inter-(SSR) PCR products as seen on gel electrophoresis reflects a true alteration within the genome.
authors.affiliation: Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
authors.forename: Neng
authors.initials: N
authors.lastname: Chen
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Stoler
authors.forename: Smitha S
authors.initials: SS
authors.lastname: Dutt
authors.forename: Gerald P
authors.initials: GP
authors.lastname: Jahreis
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Rodriguez-Bigas
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Petrelli
authors.forename: Garth R
authors.initials: GR
authors.lastname: Anderson
mesh_terms: D015139:Blotting, Southern; D015179:Colorectal Neoplasms / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D042822:Genomic Instability / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018598:Minisatellite Repeats / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*