Comparison of HeLa-I, HEp-2 and NCI-H292 cell lines for the isolation of human respiratory syncytial virus (HRSV).
Generally, laboratory diagnosis of viral respiratory infections utilizes virus isolation in cell culture and immunofluorescence assays. In this study, three cell lines (HEp-2, NCI-H292 and HeLa-I) were used for HRSV isolation of strains obtained from patients admitted at HU-USP with respiratory tract disease. HRSV was isolated in 46% (37) of 80 specimens inoculated in HeLa-I, 48% (39) in HEp-2, and 36.3% (29) in NCI-H292. Immunofluorescence was considered the gold standard and yielded 53% positive (43). The results from both methods combined had better sensitivity (73.2%) compared to either method alone. Comparing results between the cell lines with HEp-2 cells as the benchmark, the greatest sensitivity (72.2%) was observed in HeLa-I. This data shows that HeLa-I is adequate for HRSV isolation, giving results similar to the HEp-2 cells. The combined use of the HEp-2, HeLa-I and NCI-H292 cells improve the detection of HRSV.
Perini
Ana Priscila
AP
Universidade de São Paulo, Instituto de Ciências Biomédicas, São Paulo, Brazil.
Barbosa
Maria Luisa
ML
Botosso
Viviane Fongaro
VF
de Moraes
Claudia Trigo Pedroso
CT
Gillio
Alfredo E
AE
Hens
Noeli
N
Stewien
Klaus E
KE
Durigon
Edison L
EL
D045744:Cell Line, Tumor; D003588:Cytopathogenic Effect, Viral; D019084:Fluorescent Antibody Technique, Indirect; D006367:HeLa Cells; D006801:Humans; D007223:Infant; D009305:Nasopharynx / Q000821:virology; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000302:isolation & purification* / Q000502:physiology; D012680:Sensitivity and Specificity; D014776:Virus Cultivation