title: [Identification of protein antigen for antibody DBD02 by biopanning of T7 phage display library].
abstract: OBJECTIVE
  To identify the specificity of mAb DBD02, we developed and optimized a new method by biopanning of T7 Select human liver cDNA phage display library.
  
  
  METHODS
  After two rounds of biopanning, eluted phages were subjected to Dot blot using mAb DBD02 as primary antibody. The positive PFUs (plaque forming unit) which recognized by DBD02 were further confirmed by Western blot, PCR and sequencing.
  
  
  RESULTS
  The antigen recognized by DBD02 was identified as alcohol dehydrogenase. And the epitope for mAb DBD02 was further mapped within a peptide of 22 amino acids (QDYKKPIQEVLKEMTDG-GVDFS).
  
  
  CONCLUSIONS
  Our data indicate that biopanning of T7 phage display library by mAbs is time, cost, and labor-saving and specific tool for protein antigen identification.
authors.affiliation: Department of Immunology, Beijing Institute of Radiation Medicine, Beijing 100850, China. hexiang_spring@yahoo.com
authors.forename: Xiang
authors.initials: X
authors.lastname: He
authors.forename: Jin-ju
authors.initials: JJ
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiao-lan
authors.initials: XL
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Jian-en
authors.initials: JE
authors.lastname: Gao
authors.forename: Qi-hong
authors.initials: QH
authors.lastname: Sun
mesh_terms: D000426:Alcohol Dehydrogenase / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D000915:Antibody Affinity; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*; D017123:Bacteriophage T7; D015153:Blotting, Western; D015723:Gene Library; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D019151:Peptide Library; D016133:Polymerase Chain Reaction; D013997:Time Factors