[Rapid improvement of lipase production in Penicillium expansum by genome shuffling].
In the present study, the genome shuffling was used to improve lipase production of Penicillium expansum. A lipase producing mutant strain-Penicillium expansum FS8486 and a wild type of Aspergillus Tamarii FS-132 isolated from soil of a volcano in Xinjiang were used as the parental strains. After two rounds of genome shuffling, several elite daughter strains were screened. The lipase activity in one of the daughter strains was increased 317% over the starting strain FS8486. Comparisons of the morphology, RAPD (Random Amplification of Polymorphic DNA) polymorphism and the fatty acid compositions between the daughter and the parental strains suggested that the filial generation were generated by genome shuffling. In this study, the genome shuffling used successfully first time in eukaryotic microorganism and increases the production of the desired metabolite in short time, the study will be useful to spread the genome shuffling in eukaryotic microbial breeding.
Lin
Jun
J
Engineering Research Centre of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou, 350018, China.
Shi
Bi-Hong
BH
Shi
Qiao-Qin
QQ
He
Yun-Xia
YX
Wang
Ming-Zi
MZ
D001230:Aspergillus / Q000235:genetics; D032621:DNA Shuffling / Q000379:methods*; D024861:Genetic Enhancement / Q000379:methods; D016681:Genome, Fungal / Q000235:genetics*; D008049:Lipase / Q000096:biosynthesis* / Q000235:genetics; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique