Alpha-glucosidase inhibitor proteins from Sesbania grandiflora flowers.
Two alpha-glucosidase inhibitors were isolated from the flowers of Sesbania grandiflora and named SGF60 and SGF90. The procedure involved extraction with phosphate buffer, precipitation with ammonium sulfate, ion-exchange chromatography on DEAE-cellulose and gel filtration on Superdex-200. These proteins were identified by using tandem mass spectrometry. The results show partial amino acid sequences of SGF60 similar to p27SJ, a protein from Hypericum perforatum found to suppress HIV-1 gene expression. SGF90 matched a beta-glucosidase from Arabidopsis thaliana.
Boonmee
Apaporn
A
Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
Reynolds
Colin D
CD
Sangvanich
Polkit
P
D000595:Amino Acid Sequence; D018744:DNA, Plant / Q000032:analysis; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D035264:Flowers; D005959:Glucosidases / Q000037:antagonists & inhibitors* / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D010940:Plant Proteins / Q000235:genetics; D049931:Sesbania