title: [Frequency of the CD4+CD25nt/hiCD127lo regulatory T lymphocytes from the peripheral blood in Chinese healthy individuals].
abstract: OBJECTIVE
  To determine the frequency of the CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes from the peripheral blood in the Chinese healthy individuals and provide some useful evidence for clinical research of correlative diseases.
  
  
  METHODS
  From the CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes of peripheral blood in 312 Chinese healthy male and female individuals aged from 8 to 60(five age groups were collected) The expression of transcription factor Foxp3 was detected by triplex immuno fluorescence and the frequency of CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes was determined by flow cytometry.
  
  
  RESULTS
  The frequency of CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes in Chinese healthy individuals was (6.55+/-0.11)%, and the frequency differed among age groups(P=0.015) and sex groups(P<0.05). CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes specifically express transcription factor Foxp3.
  
  
  CONCLUSIONS
  The frequency of the CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes from the peripheral blood in the Chinese healthy individuals has been preliminarily determined which lays the foundation for further clinical research of regulatory T lymphocytes. As a specific cell surface marker, CD25(nt/hi)CD127(lo) can helpful obtain pure CD4(+)CD25(+) regulatory T lymphocytes and suppress the interference of other cells during cell separation.
authors.affiliation: Shanghai Municipal Center for Disease Control and Prevention, Shanghai Municipal Center for AIDS Research Director, Shanghai 200336, China. wangying2000@163.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Lei-ming
authors.initials: LM
authors.lastname: Zhou
authors.forename: Ju-li
authors.initials: JL
authors.lastname: Gong
authors.forename: Ming-kui
authors.initials: MK
authors.lastname: Zhou
authors.forename: Fang-wei
authors.initials: FW
authors.lastname: Shen
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Ming-hua
authors.initials: MH
authors.lastname: Zhuang
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Ping
authors.initials: P
authors.lastname: Zhong
authors.forename: Qi-chao
authors.initials: QC
authors.lastname: Pan
authors.forename: Lai-yi
authors.initials: LY
authors.lastname: Kang
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D044466:Asian People; D015415:Biomarkers / Q000378:metabolism; D001769:Blood / Q000378:metabolism*; D015704:CD4 Antigens / Q000378:metabolism*; D018791:CD4 Lymphocyte Count; D002648:Child; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D005786:Gene Expression Regulation; D006262:Health; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism*; D053699:Interleukin-7 Receptor alpha Subunit / Q000378:metabolism*; D008297:Male; D051379:Mice; D008875:Middle Aged; D051381:Rats; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000378:metabolism*