title: Nocardia tenerifensis genome identification in a cutaneous granuloma of a cat.
abstract: An adult Domestic Longhair cat developed a subcutaneous mass in its tail. Histologically, this mass consisted of ill-defined pyogranulomas centered around aggregates of gram-positive, acid-fast filamentous bacteria, consistent with Nocardia. Due to the lack of fresh samples, DNA was extracted from formalin-fixed paraffin-embedded tissue sections and subjected to polymerase chain reaction amplification and DNA sequencing of 16S ribosomal RNA gene encompassing Nocardia sp.-specific sequences. Sequences analyzed using the GenBank database revealed 99.5% homology with Nocardia spp. and had the highest sequence homology of 98.2% with Nocardia tenerifensis among Nocardia spp. To the authors' knowledge, this is the first report of detection of N. tenerifensis genome associated with cutaneous nocardiosis in an animal.
authors.affiliation: Animal Disease Diagnostic Laboratory and Department of Comparative Pathobiology, Purdue University, West Lafayette, IN 47907, USA. ramosja@purdue.edu
authors.forename: José A
authors.initials: JA
authors.lastname: Ramos-Vara
authors.forename: Ching Ching
authors.initials: CC
authors.lastname: Wu
authors.forename: Tsang Long
authors.initials: TL
authors.lastname: Lin
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Miller
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000382:microbiology* / Q000473:pathology; D002415:Cats; D016680:Genome, Bacterial; D006099:Granuloma / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D008297:Male; D009615:Nocardia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D009617:Nocardia Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D017192:Skin Diseases, Bacterial / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D013623:Tail / Q000473:pathology