title: Automated determination of blood metabolic acidosis, total acid phosphatase, angiotensin converting enzyme, fructosamine and urinary oxalate and citrate on the ABX Pentra 400.
abstract: We have adapted automated assays for quantification of pyruvate (PYR), acetoacetate (AA), and betahydroxybutyrate (BOHB) in plasma, total acid phosphatase (TAP), angiotensin converting enzyme (ACE) and fructosamine (FRUCT) in serum, and oxalate (OXAL) and citrate (CIT) in urine that can be performed on the ABX Pentra 400. The aim of this study was to evaluate the analytical performances of these parameters and to compare data obtained in patient samples with those obtained with the Cobas Mira. Within-run and between-run imprecisions ranged from 0.55 to 14.2 and 0.37 to 11.8%, respectively. Correlations with the Cobas Mira showed r2 coefficients ranging from 0.816 to 0.983 and linear regression slopes from 0.706 to 1.087. The ABX Pentra 400 provides precise and accurate measurement for a wide variety of analytes including PYR, AA, BOHB, TAP, ACE, FRUCT in blood samples and OXAL, CIT in urine.
authors.affiliation: Department of Biochemistry, Lapeyronie Hospital, Montpellier, France.
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Boularan
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Dupuy
authors.forename: Jean Paul
authors.initials: JP
authors.lastname: Cristol
mesh_terms: D000135:Acid Phosphatase / Q000097:blood*; D000138:Acidosis / Q000097:blood* / Q000175:diagnosis; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D002951:Citrates / Q000652:urine*; D019270:Fructosamine / Q000097:blood*; D006801:Humans; D010070:Oxalates / Q000652:urine*; D007703:Peptidyl-Dipeptidase A / Q000097:blood*