title: Modifying effects of fermented brown rice on fecal microbiota in rats.
abstract: Brown rice fermented by Aspergillus oryzae (FBRA) is a fiber-rich food. Effects of dietary administration of FBRA on rat fecal microbiota composition were examined. Male Wistar rats were fed a basal diet or a 5% FBRA- or 10% FBRA-containing diet, and fecal microbiota was analyzed by culture and terminal-restriction fragment length polymorphism (T-RFLP) analysis. The viable cell number of lactobacilli significantly increased after feeding 10% FBRA diet for 3 weeks compared with that in the basal diet group and that in the same group at the beginning of the experiment (day 0). An increase in the viable cell number of lactobacilli was also observed after feeding 10% FBRA for 12 weeks compared with the effect of a basal diet. T-RFLP analysis showed an increase in the percentage of lactobacilli cells in feces of rats fed 10% FBRA for 14 weeks. Lactobacilli strains isolated from rat feces were divided into six types based on their randomly amplified polymorphic DNA (RAPD) patterns, and they were identified as Lactobacillus reuteri, L. intestinalis and lactobacilli species based on homology of the partial sequence of 16S rDNA. FBRA contains lactic acid bacteria, but their RAPD patterns and identified species were different from those in rat feces. These results indicated that dietary FBRA increases the number of lactobacilli species already resident in the rat intestine.
authors.affiliation: Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770 8503, Japan. kataoka@basic.med.tokushima-u.ac.jp
authors.forename: Keiko
authors.initials: K
authors.lastname: Kataoka
authors.forename: Ryoko
authors.initials: R
authors.lastname: Kibe
authors.forename: Tomomi
authors.initials: T
authors.lastname: Kuwahara
authors.forename: Mari
authors.initials: M
authors.lastname: Hagiwara
authors.forename: Hideki
authors.initials: H
authors.lastname: Arimochi
authors.forename: Teruaki
authors.initials: T
authors.lastname: Iwasaki
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Benno
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Ohnishi
mesh_terms: D000818:Animals; D001236:Aspergillus oryzae; D003106:Colon / Q000187:drug effects / Q000382:microbiology; D004043:Dietary Fiber / Q000008:administration & dosage* / Q000494:pharmacology; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D007778:Lactobacillus / Q000235:genetics; D008297:Male; D009747:Nutritional Physiological Phenomena; D012275:Oryza / Q000378:metabolism* / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000145:classification / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D051381:Rats; D017208:Rats, Wistar