A CIDR-based timed embryo transfer protocol increases the pregnancy rate of lactating repeat breeder dairy cows.
This study evaluated the pregnancy rates following either a controlled internal drug release (CIDR)-based timed artificial insemination (TAI) or an embryo transfer (TET) protocol compared with that following a single PGF(2alpha) injection and AI after estrus (AIE) in lactating repeat breeder dairy cows. Fifty-three lactating dairy cows diagnosed as repeat breeders were used in this study and were randomly assigned to the following three treatments. (1) Cows, at random stages of the estrous cycle, received a CIDR device and 2 mg estradiol benzoate (EB; Day 0), a 25 mg PGF(2) (alpha) injection at the time of CIDR removal on Day 7 and a 1 mg EB injection on Day 8. The cows then received TAI 30 h (Day 9) after the second EB injection using dairy semen (TAI group, n=13). (2) Cows, at random stages of the estrous cycle, received the same hormonal treatments as in the TAI group. The cows then received TET on Day 16 using frozen-thawed blastocysts or morula embryos collected from Korean native cattle donors (TET group, n=13). (3) Cows, at the luteal phase, received a 25 mg injection of PGF(2alpha) and AIE using dairy semen (control group, n=27). The ovaries of the cows in the TET group were examined by transrectal ultrasonography to determine ovulation of the preovulatory follicles, and blood samples were collected for serum progesterone (P4) analysis. The pregnancy rate was significantly higher in the TET group (53.8%) than in the control (18.5%) or TAI (7.7%) groups (P<0.05). The ultrasonographic observations demonstrated that all the cows in the TET group ovulated the preovulatory follicles and concomitantly formed new corpora lutea. Accordingly, the mean serum P4 concentration remained constant between Day 0 and Day 7 of the luteal phase, decreased dramatically on Day 8 (P<0.01) and subsequently increased by Day 16 (P<0.01). These data suggest that the CIDR-based TET protocol can be used to effectively increase the pregnancy rate in lactating repeat breeder dairy cows.
Son
Dong-Soo
DS
Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea. sonds@rda.go.kr
Choe
Chang-Yong
CY
Cho
Sang-Rae
SR
Choi
Sun-Ho
SH
Kim
Hyun-Jong
HJ
Hur
Tai-Young
TY
Jung
Yeon-Gil
YG
Kang
Hyun-Gu
HG
Kim
Ill-Hwa
IH
D000818:Animals; D001947:Breeding; D002417:Cattle; D003612:Dairying; D004343:Drug Implants; D004624:Embryo Transfer / Q000379:methods* / Q000662:veterinary*; D004973:Estrus Synchronization; D005260:Female; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary; D007774:Lactation; D011247:Pregnancy; D018873:Pregnancy Rate; D011374:Progesterone / Q000097:blood