title: Heterologous protection in pigs induced by a plasmid-cured and crp gene-deleted Salmonella choleraesuis live vaccine.
abstract: In this study, we exploited a crp (cAMP receptor protein) gene-deleted, virulence plasmid-cured Salmonella choleraesuis mutant with decreased carbon source utilization, designated S.C.-Deltacrp/vpl(-), as a live vaccine strain. Normal weight gain with no clinical signs was observed in pigs immunized with high doses of S.C.-Deltacrp/vpl(-) live vaccine. Vaccination in pregnant sows induced high maternal antibodies, which could prevent piglets from Salmonella infection. Moreover, serial transmission of the vaccine strain in piglets produced no evidence of reversion to virulence. Furthermore, the peripheral blood mononuclear cells from immunized piglets also developed Salmonella specific T-cell proliferative response in vitro. Our results indicate that immunogenic antigens in S.C.-Deltacrp/vpl(-) can induce adequate immunity to protect pigs against challenge with a heterologous virulent strain. Thus, this mutant holds promise for the development of a new live S. choleraesuis vaccine.
authors.affiliation: Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, 1 Shuehfu Road, Neipu, Pingtung 91201, Taiwan. cychu@mail.npust.edu.tw
authors.forename: Chun-Yen
authors.initials: CY
authors.lastname: Chu
authors.forename: Shiang-Yiu
authors.initials: SY
authors.lastname: Wang
authors.forename: Zeng-Weng
authors.initials: ZW
authors.lastname: Chen
authors.forename: Maw-Sheng
authors.initials: MS
authors.lastname: Chien
authors.forename: Ji-Ping
authors.initials: JP
authors.lastname: Huang
authors.forename: Jen-Jin
authors.initials: JJ
authors.lastname: Chen
authors.forename: Li-Shian
authors.initials: LS
authors.lastname: Hong
authors.forename: Ai-Li
authors.initials: AL
authors.lastname: Shiau
authors.forename: Jeng-Liang
authors.initials: JL
authors.lastname: Tsai
authors.forename: Chao-Liang
authors.initials: CL
authors.lastname: Wu
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D049109:Cell Proliferation; D002373:Cyclic AMP Receptor Protein / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D007112:Immunity, Maternally-Acquired; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D011247:Pregnancy; D012475:Salmonella / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000503:physiopathology / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000235:genetics / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000503:physiopathology / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000276:immunology; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D014774:Virulence