Identification of bovine serum albumin as an IgE-reactive beef component in a dog with food hypersensitivity against beef.
IgE-reactive beef components were examined by an immunoblot analysis using a serum from a dog with food hypersensitivity against beef. The immunoblot analysis revealed a distinct band at approximately 66 kDa and a faint band at approximately 50 kDa. The immunoblot analysis for serum IgE reactivity to bovine serum albumin (BSA) also revealed a positive band at 66 kDa. Serum IgE reactivity to the 66-kDa protein of beef was diminished by pre-incubating the serum sample with BSA. Furthermore, a positive reaction to BSA was detected in intradermal testing in the dog. These results clearly indicated that BSA was an IgE-reactive beef component in the dog with food hypersensitivity against beef.
Ohmori
Keitaro
K
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Masuda
Kenichi
K
Kawarai
Shinpei
S
Yasuda
Nobutaka
N
Sakaguchi
Masahiro
M
Tsujimoto
Hajime
H
D000818:Animals; D002417:Cattle; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology / Q000662:veterinary*; D007073:Immunoglobulin E / Q000276:immunology*; D008460:Meat; D012710:Serum Albumin, Bovine / Q000276:immunology*