Induction of immune responses in mice after intragastric administration of Lactobacillus casei producing porcine parvovirus VP2 protein.
Lactobacillus casei ATCC 393 was selected as an antigen delivery vehicle for mucosal immunization against porcine parvovirus (PPV) infection. A 64-kDa fragment of PPV major protective antigen VP2 protein was used as the parvovirus antigen model. A recombinant Lactobacillus expressing VP2 protein was constructed with plasmid pPG611.1, where expression and localization of the VP2 protein from recombinant Lc393-rPPV-VP2 was detected via sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blotting, and immunofluorescence. Both local mucosal and systemic immune responses against PPV were induced in BALB/c mice immunized orally with the recombinant Lactobacillus expressing VP2 protein. The induced antibodies demonstrated neutralizing effects on PPV infection. These data indicated that the use of recombinant lactobacilli could be a valuable strategy for future vaccine development of PPV.
Xu
Yigang
Y
Veterinary Department, Northeast Agricultural University, Harbin 150030, People's Republic of China.
Li
Yijing
Y
D000818:Animals; D036022:Capsid Proteins / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D004333:Drug Administration Routes; D016503:Drug Delivery Systems; D018928:Immunity, Mucosal / Q000187:drug effects* / Q000276:immunology; D007780:Lacticaseibacillus casei / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000378:metabolism