[Sensitivity and specificity of ELISA based on recombinant Treponema pallidum antigen and rapid plasma reagin test in diagnosis of syphilis: a comparative study].
OBJECTIVE
To compare the sensitivity and specificity between ELISA based on the recombinant Treponema pallidum (Tp) antigen and rapid plasma reagin (RPR) test diagnosis of syphilis.


METHODS
Peripheral blood samples were collected from 249 patients, 177 males and 72 females, aged 34. Abbott Determine Syphilis TP test (ELISA based on the recombinant Tp antigen), RPR test, and Treponema pallidum haemagglutination assay (TPHA) were conducted with TPHA used as the gold standard.


RESULTS
The serum sensitivity and specificity of Abbott Determine Syphilis TP test were 100% and 98.4% respectively. The serum sensitivity and specificity of RPR test were 65.1% and 98.4% respectively.


CONCLUSIONS
The sensitivity of the rapid ELISA based on recombinant Tp antigen is significantly higher than that of RPR test.
Wang
Lin-na
LN
Department of Dermatology, Peking Union Medical College Hospital, Peking Union Medical College, Beijing 100730, China.
Zheng
He-yi
HY
Li
Jun
J
Wang
Xiao-feng
XF
Liu
Xiu-rong
XR
D000328:Adult; D000942:Antigens, Bacterial / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D011935:Reagins / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013587:Syphilis / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D013588:Syphilis Serodiagnosis / Q000379:methods*; D014210:Treponema pallidum / Q000276:immunology / Q000302:isolation & purification