Determination of plasma tenofovir concentrations using a conventional LC-MS method.
The quantification of tenofovir, a nucleoside reverse transcriptase inhibitor prescribed once daily, in human plasma is important due to a recent increase in its use. HPLC, however, can not easily detect and quantify tenofovir because of interfering peaks. Therefore, we developed a rapid and conventional LC-MS method, validated by estimating the precision and accuracy for inter- and intraday analysis in the concentration range of 0.019-1.567 microg/ml. The calibration curve was linear in the described concentration range. Average accuracy ranged from 95.9 to 100.7%. Relative standard deviations of both inter- and intraday assays were less than 11.6%. Recovery of tenofovir was more than 80.2%. This novel method provides a conventional, accurate and precise way to determine tenofovir in human plasma samples.
Takahashi
Masaaki
M
Department of Pharmacy, National Hospital Organization Nagoya Medical Center (Tokai Area Central Hospital for AIDS Treatment and Research), Naka-ku, Nagoya, Aichi 460-0001, Japan.
Kudaka
Yuichi
Y
Okumura
Naoya
N
Hirano
Atsushi
A
Banno
Kazuhide
K
Kaneda
Tsuguhiro
T
D000225:Adenine / Q000031:analogs & derivatives* / Q000097:blood; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D019380:Anti-HIV Agents / Q000097:blood*; D001262:Atenolol / Q000494:pharmacology; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D063065:Organophosphonates / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D000068698:Tenofovir