Transcriptome analysis of the Vibrio fischeri LuxR-LuxI regulon.
The Vibrio fischeri quorum-sensing signal N-3-oxohexanoyl-l-homoserine lactone (3OC6-HSL) activates expression of the seven-gene luminescence operon. We used microarrays to unveil 18 additional 3OC6-HSL-controlled genes, 3 of which had been identified by other means previously. We show most of these genes are regulated by the 3OC6-HSL-responsive transcriptional regulator LuxR directly. This demonstrates that V. fischeri quorum sensing regulates a substantial number of genes other than those involved in light production.
Antunes
Luis Caetano M
LC
Department of Microbiology, University of Iowa, Iowa City, Iowa 52242, USA.
Schaefer
Amy L
AL
Ferreira
Rosana B R
RB
Qin
Nan
N
Stevens
Ann M
AM
Ruby
Edward G
EG
Greenberg
E Peter
EP
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism; D048908:Aliivibrio fischeri / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D049449:Luminescence; D020411:Oligonucleotide Array Sequence Analysis; D009876:Operon; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D053038:Quorum Sensing; D018085:Regulon / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*