title: Anisakis simplex: the high prevalence in Madrid (Spain) and its relation with fish consumption.
abstract: Anisakiosis is a nematodosis with high prevalence in Spain. In this work we (a) investigated whether a recently introduced ELISA of Anisakis simplex-specific IgE in serum suffers from cross-reactivity with other common allergens; (b) used this assay to obtain an estimate of the prevalence of A. simplex-specific IgE in the population of Madrid; and (c) related positivity to fish consumption habits. No evidence of cross-reactivity between the ELISA and other allergens was found. The prevalence of positivity was 12.4% (11.7% among healthy subjects and 16% among patients with non-digestive non-allergic pathologies). All interviewed subjects reported consumption of uncooked fish (known to be the most likely source of infection); in addition, positivity was more prevalent among subjects who habitually consumed fresh and possibly undercooked fish than among those who generally consumed frozen fish or boiled or baked fish. These results are discussed in relation to the much lower prevalence observed in Galicia (N.W. Spain).
authors.affiliation: Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Puente
authors.forename: Ana M
authors.initials: AM
authors.lastname: Anadón
authors.forename: Marta
authors.initials: M
authors.lastname: Rodero
authors.forename: Fernanda
authors.initials: F
authors.lastname: Romarís
authors.forename: Florencio M
authors.initials: FM
authors.lastname: Ubeira
authors.forename: Carmen
authors.initials: C
authors.lastname: Cuéllar
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D017129:Anisakiasis / Q000453:epidemiology* / Q000209:etiology*; D017163:Anisakis / Q000276:immunology; D000909:Antibodies, Helminth / Q000097:blood; D003296:Cooking / Q000379:methods; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D005399:Fishes; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008875:Middle Aged; D017747:Seafood; D016036:Seroepidemiologic Studies; D013030:Spain / Q000453:epidemiology