Determination of glyceraldehyde formed in glucose degradation and glycation.
Glyceraldehyde (GLA) was determined in glucose degradation and glycation. GLA was detected as a decahydroacridine-1,8-dione derivative on reversed phase HPLC using cyclohexane-1,3-dione derivatizing reagent. The glucose-derived GLA level was higher than the glycation-derived GLA level, because GLA was converted to intermediates and advanced glycation end products (AGE) in glycation. GLA was also generated from 3-deoxyglucosone and glucosone as intermediates of glucose degradation and glycation. This study suggests that glyceraldehyde is generated by hyperglycemia in diabetes, and that it is also formed in medicines such as peritoneal dialysis solution.
Usui
Teruyuki
T
Department of Agricultural Chemistry, Faculty of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.
Yoshino
Miku
M
Watanabe
Hirohito
H
Hayase
Fumitaka
F
D002851:Chromatography, High Pressure Liquid; D005947:Glucose / Q000737:chemistry*; D005985:Glyceraldehyde / Q000737:chemistry*; D006031:Glycosylation; D007661:Ketoses / Q000737:chemistry; D015394:Molecular Structure