Molecular karyotype analysis of Perkinsus atlanticus (Phylum Perkinsozoa) by pulsed field gel electrophoresis.
Perkinsus atlanticus is a pathogenic protist that infects the clam Ruditapes decussatus. Although it was recently proposed that the genus Perkinsus belongs to a new phylum, Perkinsozoa, in the infra-kingdom Alveolata, there remain different opinions about whether this genus should form a phylum on its own and consequently divergent views about its taxonomic characterization. In this work, we have identified nine chromosomes by pulsed field gel electrophoresis (PFGE) combined with densitometry analysis. The obtained karyotype of Perkinsus atlanticus, like that of other early branches of the dinoflagellate lineage, displays a more conventional chromosome organization, different from that of most dinoflagellates.
Leonor Teles-Grilo
M
M
Molecular Genetics, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Lg Abel Salazar no 2, P-4099-003, Porto, Portugal.
Duarte
Sérgio M
SM
Tato-Costa
Joana
J
Gaspar-Maia
Alexandre
A
Oliveira
Carla
C
Rocha
António A
AA
Marques
Américo
A
Cordeiro-da-Silva
Anabela
A
Azevedo
Carlos
C
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis; D003720:Densitometry; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods / Q000662:veterinary*; D056890:Eukaryota / Q000235:genetics*; D007621:Karyotyping / Q000379:methods / Q000662:veterinary*