DNA binding and nuclease activity of a one-dimensional heterometallic nitrosyl complex.
The interaction of a structurally characterized Sr-Fe nitrosyl complex with DNA has been studied by UV-vis and fluorescence spectroscopy, viscometric, and gel electrophoresis techniques. From the absorption titration studies the intrinsic binding constant of the complex with DNA was calculated to be 1.6x10(4)M(-1). Fluorimetric studies indicate that the complex compete with EB in binding to DNA. The complex shows nuclease activity on pUC19 supercoiled DNA in presence of H(2)O(2).
Selim
Md
M
Department of Chemistry, Jadavpur University, Kolkata 700032, India.
Chowdhury
Sujoy Roy
SR
Mukherjea
Kalyan K
KK
D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000737:chemistry* / Q000378:metabolism*; D004581:Electronics; D006861:Hydrogen Peroxide; D008958:Models, Molecular; D009589:Nitrogen Oxides / Q000378:metabolism; D011487:Protein Conformation; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet