6-Aminohexanoate oligomer hydrolases from the alkalophilic bacteria Agromyces sp. strain KY5R and Kocuria sp. strain KY2.
Alkalophilic, nylon oligomer-degrading strains, Agromyces sp. and Kocuria sp., were isolated from the wastewater of a nylon-6 factory and from activated sludge from a sewage disposal plant. The 6-aminohexanoate oligomer hydrolases (NylC) from the alkalophilic strains had 95.8 to 98.6% similarity to the enzyme in neutrophilic Arthrobacter sp. but had superior thermostability, activity under alkaline conditions, and affinity for nylon-related substrates, which would be advantageous for biotechnological applications.
Yasuhira
Kengo
K
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280, Japan. negoro@eng.u-hyogo.ac.jp.
Tanaka
Yasuhito
Y
Shibata
Hiroshi
H
Kawashima
Yasuyuki
Y
Ohara
Akira
A
Kato
Dai-ichiro
D
Takeo
Masahiro
M
Negoro
Seiji
S
D000192:Actinomycetales / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D000581:Amidohydrolases / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D005798:Genes, Bacterial; D009757:Nylons / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology; D013379:Substrate Specificity