One step purification of flavanone glycosides from Poncirus trifoliata by centrifugal partition chromatography.
Flavanone glycosides were successfully separated from the crude extract of Poncirus trifoliata by preparative centrifugal partition chromatography with a two-phase solvent system composed of ethyl acetate-acetonitrile-water (3:2:5, v/v/v). Naringin (50.0 mg), neoponcirin (16.8 mg), and poncirin (71.9 mg) were purified from the 524 mg crude extract in only one step. The purities of the isolated compounds were determined to be over 90% by HPLC analysis and their structures were elucidated by (1)H NMR, (13)C NMR, and ESI-MS.
Kim
Chul Young
CY
Natural Product Research Center, KIST Gangneung Institute, Daejeon-dong, Gangneung, Gangwon-do, Korea. kcy98@kist.re.kr
Lee
Hee Ju
HJ
Lee
Mi Kyeong
MK
Ahn
Mi-Jeong
MJ
Kim
Jinwoong
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044950:Flavanones / Q000737:chemistry / Q000302:isolation & purification*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D032104:Poncirus / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet