title: Electrochemical study of the antineoplastic agent etoposide at carbon paste electrode and its determination in spiked human serum by differential pulse voltammetry.
abstract: The electrochemical oxidation of the antineoplastic agent etoposide was studied at carbon paste electrode in Britton-Robinson buffer solutions over the pH range 2.0-10.0 using cyclic, linear sweep and differential pulse voltammetry. Oxidation of the drug was effected in a single reversible, diffusion-controlled step within the pH range 2.0-4.0, a second oxidation process was produced above pH 4.0. Using differential pulse voltammetry (DPV), the drug yielded a well-defined voltammetric response in Britton-Robinson buffer, pH 3.0 at 0.500 V (vs. Ag/AgCl) on carbon paste electrode. This process could be used to determine etoposide concentrations in the range 2.5 x 10(-7) to 2.5 x 10(-5) M with a detection limit of 1.0 x 10(-7) M. The method was successfully applied to the determination of the drug in spiked human serum.
authors.affiliation: Department of Chemistry, Faculty of Science (Dumyat), Mansoura University, Dumyat, Egypt. abradi@yahoo.com
authors.forename: Abd-Elgawad
authors.initials: AE
authors.lastname: Radi
authors.forename: Nadia
authors.initials: N
authors.lastname: Abd-Elghany
authors.forename: Tarek
authors.initials: T
authors.lastname: Wahdan
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000097:blood / Q000737:chemistry*; D002244:Carbon; D004563:Electrochemistry; D004566:Electrodes; D005047:Etoposide / Q000097:blood / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D010084:Oxidation-Reduction