title: Virus infection of culturable chlorella-like algae and dlevelopment of a plaque assay.
abstract: Four distinct viruses with double-stranded DNA are known to replicate in Chlorella-like algae symbiotic with hydras and paramecia. An attempt was made to infect a number of cultured Chlorella strains derived from invertebrate hosts with these viruses. One of the viruses, PBCV-1, replicated in two of the algal strains. Restriction endonuclease analysis of the viral DNA showed that the infectious progeny virus was identical to the input virus; thus, Koch's postulates were fulfilled. Viral infection of the two Chlorella strains has allowed the large-scale production of a eukaryotic algal virus and the development of a plaque assay for the virus.
authors.forename: J L
authors.initials: JL
authors.lastname: VAN Etten
authors.forename: D E
authors.initials: DE
authors.lastname: Burbank
authors.forename: D
authors.initials: D
authors.lastname: Kuczmarski
authors.forename: R H
authors.initials: RH
authors.lastname: Meints