title: DNA binding and nuclease activity of a one-dimensional heterometallic nitrosyl complex.
abstract: The interaction of a structurally characterized Sr-Fe nitrosyl complex with DNA has been studied by UV-vis and fluorescence spectroscopy, viscometric, and gel electrophoresis techniques. From the absorption titration studies the intrinsic binding constant of the complex with DNA was calculated to be 1.6x10(4)M(-1). Fluorimetric studies indicate that the complex compete with EB in binding to DNA. The complex shows nuclease activity on pUC19 supercoiled DNA in presence of H(2)O(2).
authors.affiliation: Department of Chemistry, Jadavpur University, Kolkata 700032, India.
authors.forename: Md
authors.initials: M
authors.lastname: Selim
authors.forename: Sujoy Roy
authors.initials: SR
authors.lastname: Chowdhury
authors.forename: Kalyan K
authors.initials: KK
authors.lastname: Mukherjea
mesh_terms: D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000737:chemistry* / Q000378:metabolism*; D004581:Electronics; D006861:Hydrogen Peroxide; D008958:Models, Molecular; D009589:Nitrogen Oxides / Q000378:metabolism; D011487:Protein Conformation; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet