title: Expression of junctional adhesion molecules on the human lymphatic endothelium.
abstract: Human lymphatic vessels express several leukocyte adhesion molecules. The study here investigated the expression of three junctional adhesion molecules (JAM) which are a newly reported glycoprotein family of adhesion molecules on human lymphatic endothelium. In this study, JAM-1 and JAM-3 but not JAM-2 were detected in cultured human neonatal dermal lymphatic endothelial cells (LEC) at the gene and protein levels by microarray, RT-PCR, real-time PCR, and immunohistochemical analysis. The JAM-1 and JAM-3 expression was not altered in the TNF-alpha-treated LEC or in the untreated cells. In human tissue, the expression of JAM-1, and the expression of JAM-1, JAM-2, and JAM-3 were observed in collecting lymphatic vessels of uninflamed small intestine, and in initial lymphatics of inflamed tongue and uninflamed gingival tissue. It is thought that JAM-2 mRNA could be produced in mature vascular endothelium but not in cultured cells, and that human intestinal and oral lymphatic vessels usually express JAM-1, JAM-2, and JAM-3. There were initial lymphatics simultaneously expressing JAM-1, JAM-2, and JAM-3 in the mucosal connective tissue papillae of gingival tissue. The three JAM expressions on the lymphatic endothelium may contribute to both seal the cell-cell contact at interendothelial junctions and also allow lymphocytes to transmigrate into lymphatic vessels from tissue, independent of inflammatory cytokines.
authors.affiliation: Department of Oral Growth and Development, Fukuoka Dental College, 2-15-1 Tamura, Sawara-Ku, Fukuoka, 814-0193, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ueki
authors.forename: Kana
authors.initials: K
authors.lastname: Iwasawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Sawa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D004729:Endothelium, Lymphatic / Q000378:metabolism* / Q000473:pathology; D004751:Enteritis; D005881:Gingiva / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000378:metabolism* / Q000473:pathology; D007421:Intestine, Small / Q000378:metabolism; D062726:Junctional Adhesion Molecules; D008875:Middle Aged; D010518:Periodontitis / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D019108:Tight Junctions / Q000378:metabolism* / Q000473:pathology; D014059:Tongue / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D062765:Junctional Adhesion Molecule B