Comparison of time-resolved fluoroimmunoassay for determining hexoestrol residues in chicken muscle tissues based on polyclonal antibodies with liquid chromatography and tandem mass spectrometry.
A time-resolved fluoroimmunoassay (TR-FIA) was developed for the determination of hexoestrol (HES) residues in animal tissues. The limit of detection (LOD) was determined to be 0.02 ng g(-1) and the limit of quantification (LOQ) was less than 0.12 ng g(-1). The results obtained by the TR-FIA and ELISA showed a good correlation. The established TR-FIA was validated for the determination of market chicken muscle tissues and confirmed by high-performance liquid chromatography and tandem mass spectrometry (LC-MS-MS). This proposed technique could be applied to routine residue analysis.
Xiangqian
Li
L
Department of Chemical and Bioengineering, Huaiyin Institute of Technology, 223001, Huai'an, JiangSu Province, China. xiangqianli2004@163.com
Yongming
Hu
H
Tieming
Huo
H
Xiaogang
Chu
C
Chuanlai
Xu
X
D000818:Animals; D000906:Antibodies; D002645:Chickens; D002853:Chromatography, Liquid / Q000592:standards; D004350:Drug Residues / Q000032:analysis*; D015200:Fluoroimmunoassay / Q000379:methods / Q000592:standards*; D006589:Hexestrol / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000592:standards