Sequencing of avian influenza virus genomes following random amplification.
Increasing surveillance for the avian influenza virus (AIV) has underscored the need for quickly and precisely characterizing isolates of this highly variable target. Random amplification, sequencing, and assembly of total RNA from nonpurified virus overcomes the need for specific primers for DNA microarray or PCR protocols.
Afonso
Claudio L
CL
U.S. Department of Agriculture, Athens, CA, USA. cafonso@seprl.usda.gov
D000818:Animals; D001483:Base Sequence; D001717:Birds; D002874:Chromosome Mapping / Q000379:methods*; D016679:Genome, Viral / Q000235:genetics*; D009980:Influenza A virus / Q000235:genetics*; D005585:Influenza in Birds / Q000821:virology; D008969:Molecular Sequence Data; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*