[Effect of PF4 on 60Co gamma ray irradiation-induced bone marrow cell apoptosis in mice].
OBJECTIVE
To study the effect of platelet factor 4 (PF4) on murine bone marrow cell apoptosis after irradiation with 5.0 Gy (60)COgamma and explore its potential mechanism.


METHODS
30 male BALB/c mice were randomly divided into three groups: namely normal control group (n = 6), PF4 treatment group (n=12) and irradiation (IR) group (n = 12). The mice in PF4 treatment group were administrated intraperitoneally PF4 40 microg/kg at 26 h and 20 h respectively before total-body irradiation with 5.0 Gy (60)COgamma ray. At 4 h and 20 h after irradiation the apoptosis of bone marrow cells were detected by flow cytometry (FCM) and the expression of Bax protein was identified by Western blot.


RESULTS
At 4 h and 20 h after irradiation the apoptosis of bone marrow cells in PF4 treatment group was significantly lower than that in IR group (P < 0.01), the expression of Bax protein in the PF4 group was lower than that in the IR group (P < 0.05).


CONCLUSIONS
PF4 can protect bone marrow cells from radiation-induced apoptosis, which is partly due to the down-regulation of proapoptotic protein Bax expression.
Lin
Hui-liang
HL
Department of Hematology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. 314lhl@163.com
Yang
Lan
L
Wang
Xin-rong
XR
Gu
Hong-tao
HT
Gao
Guang-xun
GX
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects; D003037:Cobalt Radioisotopes / Q000009:adverse effects; D005434:Flow Cytometry; D005720:Gamma Rays / Q000009:adverse effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010978:Platelet Factor 4 / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism