Growth activity of epidermal cells from different parts of human body.
BACKGROUND
Most epidermal cells used in skin tissue engineering are obtained from the skins of fetuses or prepuces, which can not be widely used in culturing and transplanting autologous epidermis for patients with extensive burn wounds. To solve the problem, in this study, we cultured epidermal cells from different parts of human body in vitro, and detected their growth activity.


METHODS
Normal epidermal cells obtained from the prepuce, scalp, and axilla of male patients, were cultured and passaged. Their growth characteristics including adherent rate and growth activity were compared. Data were analyzed by homogeneity test of variance.


RESULTS
In primary culture, the growth of epidermal cells from the prepuce was significantly faster than that of the epidermal cells from the scalp and axilla. In the cells obtained from the prepuce, 80% confluence was achieved on day 12, while on day 16 and day 20 in the cells from the scalp and axilla, respectively. However, no significant difference was detected in their growth and proliferation in the second passage.


CONCLUSIONS
Although the growth of epidermal cells obtained from the scalp and axilla is slower than that from the prepuce in primary culture, stable cell line can be established and used in preparation of auto-epidermal grafts for patients with extensive burn wounds. Therefore, the scalp and axillary skin should be considered as important sources of epidermal cells other than the prepuce.
Chai
Jia-ke
JK
Burns Institute, First Hospital Affiliated to General Hospital of PLA, Beijing 100037, China. cjk304@126.com
Sheng
Zhi-yong
ZY
Ma
Zhong-feng
ZF
Yang
Hong-ming
HM
Liu
Qiang
Q
Liang
Li-ming
LM
D000328:Adult; D049109:Cell Proliferation; D002470:Cell Survival; D000078404:Epidermal Cells; D006801:Humans; D008297:Male; D014357:Trypsin / Q000494:pharmacology