title: Comparison of time-resolved fluoroimmunoassay for determining hexoestrol residues in chicken muscle tissues based on polyclonal antibodies with liquid chromatography and tandem mass spectrometry.
abstract: A time-resolved fluoroimmunoassay (TR-FIA) was developed for the determination of hexoestrol (HES) residues in animal tissues. The limit of detection (LOD) was determined to be 0.02 ng g(-1) and the limit of quantification (LOQ) was less than 0.12 ng g(-1). The results obtained by the TR-FIA and ELISA showed a good correlation. The established TR-FIA was validated for the determination of market chicken muscle tissues and confirmed by high-performance liquid chromatography and tandem mass spectrometry (LC-MS-MS). This proposed technique could be applied to routine residue analysis.
authors.affiliation: Department of Chemical and Bioengineering, Huaiyin Institute of Technology, 223001, Huai'an, JiangSu Province, China. xiangqianli2004@163.com
authors.forename: Li
authors.initials: L
authors.lastname: Xiangqian
authors.forename: Hu
authors.initials: H
authors.lastname: Yongming
authors.forename: Huo
authors.initials: H
authors.lastname: Tieming
authors.forename: Chu
authors.initials: C
authors.lastname: Xiaogang
authors.forename: Xu
authors.initials: X
authors.lastname: Chuanlai
mesh_terms: D000818:Animals; D000906:Antibodies; D002645:Chickens; D002853:Chromatography, Liquid / Q000592:standards; D004350:Drug Residues / Q000032:analysis*; D015200:Fluoroimmunoassay / Q000379:methods / Q000592:standards*; D006589:Hexestrol / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000592:standards