Expression and subcellular localization of E-cadherin, alpha-catenin, and beta-catenin in 8 feline mammary tumor cell lines.
Protein expression and subcellular localization of E-cadherin, alpha-catenin, and beta-catenin in 8 feline mammary tumor cell lines were examined by western blot analysis and fluorescence immunocytochemistry. A low E-cadherin expression was observed in FNN-m cells. Furthermore, compared to other cell lines, two E-cadherin bands existed in FMC-p1 cells and were localized in the perinuclear region; distinct radial lines were observed in the cytoplasm. A low alpha-catenin expression was observed in FON-m cells, but there were no apparent abnormalities in its localization. In contrast, similar levels of beta-catenin expression and cytoplasmic localization were observed in all cell lines.
Takauji
Shun-roku
SR
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, Tokyo, Japan.
Watanabe
Manabu
M
Uyama
Rina
R
Nakagawa
Takayuki
T
Miyajima
Nozomi
N
Mochizuki
Manabu
M
Nishimura
Ryohei
R
Sugano
Sumio
S
Sasaki
Nobuo
N
D000818:Animals; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D002415:Cats; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D015674:Mammary Neoplasms, Animal / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D051178:alpha Catenin / Q000235:genetics* / Q000378:metabolism; D051176:beta Catenin / Q000235:genetics* / Q000378:metabolism