title: Comparison of HeLa-I, HEp-2 and NCI-H292 cell lines for the isolation of human respiratory syncytial virus (HRSV).
abstract: Generally, laboratory diagnosis of viral respiratory infections utilizes virus isolation in cell culture and immunofluorescence assays. In this study, three cell lines (HEp-2, NCI-H292 and HeLa-I) were used for HRSV isolation of strains obtained from patients admitted at HU-USP with respiratory tract disease. HRSV was isolated in 46% (37) of 80 specimens inoculated in HeLa-I, 48% (39) in HEp-2, and 36.3% (29) in NCI-H292. Immunofluorescence was considered the gold standard and yielded 53% positive (43). The results from both methods combined had better sensitivity (73.2%) compared to either method alone. Comparing results between the cell lines with HEp-2 cells as the benchmark, the greatest sensitivity (72.2%) was observed in HeLa-I. This data shows that HeLa-I is adequate for HRSV isolation, giving results similar to the HEp-2 cells. The combined use of the HEp-2, HeLa-I and NCI-H292 cells improve the detection of HRSV.
authors.affiliation: Universidade de São Paulo, Instituto de Ciências Biomédicas, São Paulo, Brazil.
authors.forename: Ana Priscila
authors.initials: AP
authors.lastname: Perini
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Barbosa
authors.forename: Viviane Fongaro
authors.initials: VF
authors.lastname: Botosso
authors.forename: Claudia Trigo Pedroso
authors.initials: CT
authors.lastname: de Moraes
authors.forename: Alfredo E
authors.initials: AE
authors.lastname: Gillio
authors.forename: Noeli
authors.initials: N
authors.lastname: Hens
authors.forename: Klaus E
authors.initials: KE
authors.lastname: Stewien
authors.forename: Edison L
authors.initials: EL
authors.lastname: Durigon
mesh_terms: D045744:Cell Line, Tumor; D003588:Cytopathogenic Effect, Viral; D019084:Fluorescent Antibody Technique, Indirect; D006367:HeLa Cells; D006801:Humans; D007223:Infant; D009305:Nasopharynx / Q000821:virology; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000302:isolation & purification* / Q000502:physiology; D012680:Sensitivity and Specificity; D014776:Virus Cultivation