title: Cholinesterase inhibiting and antiplasmodial steroidal alkaloids from Sarcococca hookeriana.
abstract: Bioguided phytochemical investigation of Sarcococca hookeriana with respect to the cholinesterase enzyme inhibitory assay yielded two new pregnane-type steriodal alkaloids hookerianamide H (1) and hookerianamide I (2), along with three known alkaloids N(a)-methylepipachysamine D (3), sarcovagine C (4) and dictyophlebine (5). Their structures were determined with the aid of extensive spectroscopic analysis. All compounds showed good inhibitory activities against the enzymes acetylcholinesterase (IC(50) 2.9-34.1 microM) and butyrylcholinesterase (IC(50) 0.3-3.6 microM). These compounds also showed moderate antiplasmodial activity (IC(50) 2.4-10.3 microM) against the Plasmodium falciparum chloroquine resistant W2 strain.
authors.affiliation: Department of Chemistry, Organic and Bioorganic Chemistry, Bielefeld University, Bielefeld, Germany. devkotakpd@yahoo.com
authors.forename: Krishna Prasad
authors.initials: KP
authors.lastname: Devkota
authors.forename: Bruno Ndjakou
authors.initials: BN
authors.lastname: Lenta
authors.forename: Muhammad Iqbal
authors.initials: MI
authors.lastname: Choudhary
authors.forename: Qamar
authors.initials: Q
authors.lastname: Naz
authors.forename: Fabrice Boyom
authors.initials: FB
authors.lastname: Fekam
authors.forename: Philip Joseph
authors.initials: PJ
authors.lastname: Rosenthal
authors.forename: Norbert
authors.initials: N
authors.lastname: Sewald
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D000962:Antimalarials; D002091:Butyrylcholinesterase / Q000378:metabolism; D031234:Buxaceae / Q000737:chemistry*; D002800:Cholinesterase Inhibitors; D004351:Drug Resistance; D009682:Magnetic Resonance Spectroscopy; D010963:Plasmodium falciparum / Q000187:drug effects; D011278:Pregnanes / Q000302:isolation & purification / Q000494:pharmacology*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013256:Steroids / Q000302:isolation & purification* / Q000494:pharmacology*