title: [Cyclin E overexpression and centrosome amplification in squamous cell carcinoma of oral cavity].
abstract: OBJECTIVE
  To study the correlation between cyclin E protein overexpression and centrosome amplification in oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  Formalin-fixed, paraffin-embedded tissues from 12 normal oral epithelium cases and 46 cases of OSCC were studied. Their centrosome status was analyzed by indirect immunofluorescence double staining with antibodies to centrosome protein gamma-tubulin and cytokeratin. The expression of cyclin E protein was studied by immunohistochemical methods. The correlation between cyclin E protein expression and centrosome amplification in OSCC was statistically analyzed by SPSS 12.0.
  
  
  RESULTS
  Thirty-seven of the 46 OSCC cases (80.4%) studied showed evidence of centrosome amplification, as signified by enlargement and/or increase in number of centrosomes, while normal oral epithelium possessed centromeres of normal size and number. Positive staining for cyclin E protein was observed in 30 of the 46 OSCC cases (65.2%), while all the normal oral epithelium cases were cyclin E protein-negative. The percentage of centrosome amplification in OSCC with positive cyclin E protein staining (90.0%, 27/30) was higher than that in OSCC with negative cyclin E protein staining (62.5%, 10/16) (chi(2) = 5.014, P < 0.05). Centrosome amplification showed positive correlation with cyclin E protein overexpression (r = 0.330, P < 0.05).
  
  
  CONCLUSIONS
  Up-regulation of cyclin E protein may represent one of the possible mechanisms for centrosome amplification in OSCC.
authors.affiliation: Department of Stomatology, Guiyang Medical College, Guiyang 550004, China. caiyang85@163.com
authors.forename: Yang
authors.initials: Y
authors.lastname: Cai
authors.forename: Yong-fa
authors.initials: YF
authors.lastname: Liu
authors.forename: Shi-ling
authors.initials: SL
authors.lastname: Li
authors.forename: Yu-xia
authors.initials: YX
authors.lastname: Pan
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.forename: Yan-ni
authors.initials: YN
authors.lastname: Yu
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D018385:Centrosome / Q000473:pathology* / Q000648:ultrastructure; D019927:Cyclin E / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D009061:Mouth Mucosa / Q000378:metabolism / Q000473:pathology*; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D015854:Up-Regulation