title: Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes.
abstract: OBJECTIVE
  The aim of this study was to identify differential expression of microRNAs (miRNAs) in chorioamniotic membranes with advancing gestation, labor, and inflammation.
  
  
  METHODS
  Expression profiles of 157 miRNAs in the chorioamniotic membranes were obtained from patients in the following groups: 1) term not in labor (n = 10); 2) term in labor (n = 10); 3) preterm labor with histologic chorioamionitis (n = 9); and 4) without histologic chorioamnionitis (n = 10).
  
  
  RESULTS
  More than 95% of the miRNAs screened were expressed. Gestational age-dependent changes in expression were observed for 13 miRNAs. No differences in miRNA expression were observed between women without labor and women in labor. Membranes with chorioamnionitis displayed increased expression of miR-223 and miR-338. Gene Ontology analysis of genes targeted by differentially expressed miRNAs revealed enrichment for specific biological process categories.
  
  
  CONCLUSIONS
  Chorioamniotic membranes with advancing gestational age and chorioamnionitis are associated with the differential expression of a subset of miRNAs.
authors.affiliation: Perinatology Research Branch, National Institute of Child Health and Human Development/National Institutes of Health/Department of Health and Human Services, Bethesda, MD, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Montenegro
authors.forename: Roberto
authors.initials: R
authors.lastname: Romero
authors.forename: Beth L
authors.initials: BL
authors.lastname: Pineles
authors.forename: Adi L
authors.initials: AL
authors.lastname: Tarca
authors.forename: Yeon Mee
authors.initials: YM
authors.lastname: Kim
authors.forename: Sorin
authors.initials: S
authors.lastname: Draghici
authors.forename: Juan Pedro
authors.initials: JP
authors.lastname: Kusanovic
authors.forename: Jung-Sun
authors.initials: JS
authors.lastname: Kim
authors.forename: Offer
authors.initials: O
authors.lastname: Erez
authors.forename: Shali
authors.initials: S
authors.lastname: Mazaki-Tovi
authors.forename: Sonia
authors.initials: S
authors.lastname: Hassan
authors.forename: Jimmy
authors.initials: J
authors.lastname: Espinoza
authors.forename: Chong Jai
authors.initials: CJ
authors.lastname: Kim
mesh_terms: D000293:Adolescent; D000328:Adult; D000650:Amnion / Q000502:physiology; D002821:Chorioamnionitis / Q000235:genetics* / Q000473:pathology; D002823:Chorion / Q000502:physiology; D003430:Cross-Sectional Studies; D005321:Extraembryonic Membranes / Q000502:physiology* / Q000503:physiopathology; D005260:Female; D020869:Gene Expression Profiling; D005865:Gestational Age; D006801:Humans; D007249:Inflammation / Q000235:genetics; D007743:Labor, Obstetric / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D011247:Pregnancy