title: Differential chain-length specificities of two isoamylase-type starch-debranching enzymes from developing seeds of kidney bean.
abstract: Plant isoamylase-type starch-debranching enzymes (ISAs) hydrolyze alpha-1,6-linkages in alpha-1,4/alpha-1,6-linked polyglucans. Two ISAs, designated PvISA1/2 and PvISA3, were purified from developing seeds of kidney bean by ammonium sulfate fractionation and several column chromatographic procedures. The enzymes displayed different substrate specificities for polyglucans: PvISA1/2 showed broad chain-length specificities, whereas PvISA3 liberated specific chains with a DP of 2 to 4.
authors.affiliation: Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Takashima
authors.forename: Takeshi
authors.initials: T
authors.lastname: Senoura
authors.forename: Takayuki
authors.initials: T
authors.lastname: Yoshizaki
authors.forename: Shigeki
authors.initials: S
authors.lastname: Hamada
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ito
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Matsui
mesh_terms: D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D007517:Isoamylase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D027805:Phaseolus / Q000201:enzymology* / Q000235:genetics; D012639:Seeds / Q000201:enzymology / Q000235:genetics; D013213:Starch / Q000378:metabolism*; D013379:Substrate Specificity; D014158:Transcription, Genetic / Q000235:genetics