Imaging of a beta-peptide distribution in whole-body mice sections by MALDI mass spectrometry.
Mass spectrometric imaging was applied to assess compound distributions on whole-body sections of mice after i.v. dosing of a beta-peptide and an alpha-peptide control. Animals were sacrificed at different time points (5 min, 1 h, 24 h) post-dose, providing simultaneous spatial as well as kinetic information. As a result of this study, no detection of the alpha-peptide control was observed at 1 h post-dose, while retention of the beta-peptide was observed for longer than 24 h post-dose.
Stoeckli
Markus
M
Novartis Institutes for BioMedical Research, Basel, Switzerland. markus.stoeckli@novartis.com
Staab
Dieter
D
Schweitzer
Alain
A
Gardiner
James
J
Seebach
Dieter
D
D000818:Animals; D007091:Image Processing, Computer-Assisted; D007668:Kidney / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009842:Oligopeptides / Q000032:analysis / Q000493:pharmacokinetics*; D010446:Peptide Fragments / Q000032:analysis / Q000493:pharmacokinetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors; D014018:Tissue Distribution