Determination of digoxin in human serum using stable isotope dilution liquid chromatography/electrospray ionization-tandem mass spectrometry.
A method for the determination of digoxin in human serum using a liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) technique is reported. Digoxin and the internal standard, [21,21,22-(2)H(3)]digoxin, were extracted from 250 mul of human serum using a solid phase extraction cartridge (Oasis HLB) and analyzed by LC/ESI-MS/MS in the selected reaction monitoring mode. The intra- and inter-assay reproducibility and accuracy were satisfactory within the quantification range of 0.20-3.20 ng/ml. The concentrations of digoxin in the serum samples obtained from digitalized patients (n=19) were in the range of 0.25-2.84 ng/ml, which were compared to those obtained by radioimmunoassay.
Mitamura
Kuniko
K
Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
Horikawa
Aki
A
Yamane
Yuko
Y
Ikeda
Yukari
Y
Fujii
Youichi
Y
Shimada
Kazutake
K
D002138:Calibration; D002853:Chromatography, Liquid; D004077:Digoxin / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D011863:Radioimmunoassay; D011865:Radioisotope Dilution Technique; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization