B7-H3 regulates the development of experimental allergic conjunctivitis in mice.
B7-H3 negatively regulates Th1-mediated immune responses. Here, we aimed to investigate whether B7-H3 is involved in the development of murine experimental allergic conjunctivitis (EC), which is predominantly mediated by Th2 cells. Intraperitoneal injection of anti-B7-H3 Ab during the induction phase of EC significantly augmented the severity of EC evaluated as conjunctival eosinophil numbers and Ag-induced IL-5 production by splenocytes. Injection of anti-B7-H3 Ab during the effector phase of EC did not significantly affect the severity of EC. In addition, transfer of Ag-primed splenocytes treated with anti-B7-H3 Ab in vitro did not significantly affect the severity of EC, compared to the splenocytes treated with the control Ab. Thus, regulation of EC by blocking of B7-H3 was observed during the induction phase but not the effector phase. Moreover, this study provides a new notion that B7-H3 regulates not only Th1-mediated but also Th2-mediated immune reactions.
Fukushima
Atsuki
A
Department of Ophthalmology and Visual Science, Kochi Medical School, Kohasu, Oko-cho, Nankoku-city 783-8505, Japan. fukusima@kochi-u.ac.jp
Sumi
Tamaki
T
Fukuda
Ken
K
Kumagai
Naoki
N
Nishida
Teruo
T
Yamazaki
Tomohide
T
Akiba
Hisaya
H
Okumura
Ko
K
Yagita
Hideo
H
Ueno
Hisayuki
H
D000818:Animals; D060887:B7 Antigens; D018122:B7-1 Antigen / Q000235:genetics / Q000276:immunology / Q000502:physiology; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003233:Conjunctivitis, Allergic / Q000276:immunology* / Q000378:metabolism; D004195:Disease Models, Animal; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism