Effects of various cyclodextrins on the stability of freeze-dried lactate dehydrogenase.
The effects of eight cyclodextrins (CDs) on the stability of freeze-dried lactate dehydrogenase (LDH) were investigated. Five low-molecular weight saccharides were used as a reference. In the samples without saccharides, LDH activity was decreased by freeze-drying. The LDH activities of the samples with 2-hydroxypropylated CDs (HP-CDs) such as 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD) showed only a small decrease and were more effective protein stabilizers than low-molecular weight saccharides. HP-beta-CD more effectively stabilized LDH than trehalose, at a sugar concentration of less than 1.0 w/w% and furthermore, a close relationship existed between the highly stabilizing effect and the degree of substitution of polar substituents.
Iwai
Junya
J
Department of Physical Chemistry, Hoshi University, 4-41, Ebara 2-chome, Tokyo 142-8501, Japan.
Ogawa
Noriko
N
Nagase
Hiromasa
H
Endo
Tomohiro
T
Loftsson
Thorsteinn
T
Ueda
Haruhisa
H
D003505:Cyclodextrins / Q000737:chemistry*; D004795:Enzyme Stability; D005612:Freeze Drying; D007770:L-Lactate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry