title: Localization and regulation of mouse pantothenate kinase 2.
abstract: Coenzyme A (CoA) biosynthesis is initiated by pantothenate kinase (PanK) and CoA levels are controlled through differential expression and feedback regulation of PanK isoforms. PanK2 is a mitochondrial protein in humans, but comparative genomics revealed that acquisition of a mitochondrial targeting signal was limited to primates. Human and mouse PanK2 possessed similar biochemical properties, with inhibition by acetyl-CoA and activation by palmitoylcarnitine. Mouse PanK2 localized in the cytosol, and the expression of PanK2 was higher in human brain compared to mouse brain. Differences in expression and subcellular localization should be considered in developing a mouse model for human PanK2 deficiency.
authors.affiliation: Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN 38105, United States.
authors.forename: Roberta
authors.initials: R
authors.lastname: Leonardi
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Zhang
authors.forename: Athanasios
authors.initials: A
authors.lastname: Lykidis
authors.forename: Charles O
authors.initials: CO
authors.lastname: Rock
authors.forename: Suzanne
authors.initials: S
authors.lastname: Jackowski
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000235:genetics; D005091:Exons / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D016678:Genome / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D051379:Mice; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*