Effects of atelocollagen gel containing bone marrow-derived stromal cells on repair of osteochondral defect in a dog.
To clarify the contribution of autologous transplantation of mesenchymal stromal cells (MSCs), an atelocollagen gel containing or not containing fluorescently-labeled canine MSCs was transplanted into an osteochondral defect which did not repair spontaneously and the histological repair of the defect was compared. Although an early repair of the cartilage was not observed in either defect, the reproduction of subchondral bone was remarkable in the MSCs-implanted defect. Moreover, in 2 weeks after operation, the implanted MSCs were located in the deeper regions of the defect, suggesting the differentiation of osteoblasts. There was a possibility that the movement of the implanted MSCs was due to an increase in intra-articular pressure from postoperative inflammation.
Yamazoe
Kazuaki
K
Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan. yamazoe@gifu-u.ac.jp
Mishima
Hiroyuki
H
Torigoe
Kentaro
K
Iijima
Hisashi
H
Watanabe
Kazuhiro
K
Sakai
Hiroki
H
Kudo
Tadaaki
T
D000818:Animals; D003094:Collagen / Q000494:pharmacology*; D004283:Dog Diseases / Q000188:drug therapy*; D004285:Dogs; D006614:Hindlimb; D007592:Joint Diseases / Q000628:therapy / Q000662:veterinary; D010007:Osteochondritis / Q000628:therapy / Q000662:veterinary*; D017154:Stromal Cells / Q000378:metabolism*; D013997:Time Factors