title: Survival of Mycobacterium avium subsp. paratuberculosis in bovine monocyte-derived macrophages is not affected by host infection status but depends on the infecting bacterial genotype.
abstract: In this study we investigated the ability of different Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) strains to survive in bovine monocyte-derived macrophages (MDMs) of cows naturally infected with M. paratuberculosis and control cows. We tested the hypotheses that infection status of cows affects macrophage killing ability and that survival of M. paratuberculosis in macrophages is dependent on the strain. Peripheral blood mononuclear cells (PBMC) were obtained from Johne's disease-positive (n=3) and age and stage of lactation matched Johne's disease-negative (n=3) multiparious cows. Following differentiation, MDMs were challenged in vitro with four M. paratuberculosis strains of different host specificity (cattle and sheep). Two hours and 2, 4, and 7 days after infection, ingestion, and intracellular survival of M. paratuberculosis strains were determined by fluorescence microscopy. There was no effect of the origin of MDMs (Johne's disease-positive or control animals) on phagocytosis, survival of bacteria, or macrophage survival. In contrast, important strain differences were observed. These findings suggest that some M. paratuberculosis strains interfere more successfully than others with the ability of macrophages to kill intracellular pathogens which may make it important to include strain typing when designing control programs.
authors.affiliation: Clinic for Ruminants, University of Munich, Sonnenstrasse 16, 85764 Oberschleissheim, Germany. nicole.gollnick@med.vetmed.uni-muenchen.de <nicole.gollnick@med.vetmed.uni-muenchen.de>
authors.forename: Nicole S
authors.initials: NS
authors.lastname: Gollnick
authors.forename: Rebecca M
authors.initials: RM
authors.lastname: Mitchell
authors.forename: Martin
authors.initials: M
authors.lastname: Baumgart
authors.forename: Harish K
authors.initials: HK
authors.lastname: Janagama
authors.forename: Srinand
authors.initials: S
authors.lastname: Sreevatsan
authors.forename: Ynte H
authors.initials: YH
authors.lastname: Schukken
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005260:Female; D005434:Flow Cytometry; D005838:Genotype; D008264:Macrophages / Q000166:cytology / Q000382:microbiology*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics* / Q000502:physiology*; D010283:Paratuberculosis / Q000276:immunology / Q000382:microbiology*; D013997:Time Factors