[Frequency of the CD4+CD25nt/hiCD127lo regulatory T lymphocytes from the peripheral blood in Chinese healthy individuals].
OBJECTIVE
To determine the frequency of the CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes from the peripheral blood in the Chinese healthy individuals and provide some useful evidence for clinical research of correlative diseases.


METHODS
From the CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes of peripheral blood in 312 Chinese healthy male and female individuals aged from 8 to 60(five age groups were collected) The expression of transcription factor Foxp3 was detected by triplex immuno fluorescence and the frequency of CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes was determined by flow cytometry.


RESULTS
The frequency of CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes in Chinese healthy individuals was (6.55+/-0.11)%, and the frequency differed among age groups(P=0.015) and sex groups(P<0.05). CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes specifically express transcription factor Foxp3.


CONCLUSIONS
The frequency of the CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes from the peripheral blood in the Chinese healthy individuals has been preliminarily determined which lays the foundation for further clinical research of regulatory T lymphocytes. As a specific cell surface marker, CD25(nt/hi)CD127(lo) can helpful obtain pure CD4(+)CD25(+) regulatory T lymphocytes and suppress the interference of other cells during cell separation.
Wang
Ying
Y
Shanghai Municipal Center for Disease Control and Prevention, Shanghai Municipal Center for AIDS Research Director, Shanghai 200336, China. wangying2000@163.com
Zhou
Lei-ming
LM
Gong
Ju-li
JL
Zhou
Ming-kui
MK
Shen
Fang-wei
FW
Zheng
Xiao-hong
XH
Zhang
Wei
W
Zhuang
Ming-hua
MH
Lu
Wei
W
Zhong
Ping
P
Pan
Qi-chao
QC
Kang
Lai-yi
LY
D000293:Adolescent; D000328:Adult; D000818:Animals; D044466:Asian People; D015415:Biomarkers / Q000378:metabolism; D001769:Blood / Q000378:metabolism*; D015704:CD4 Antigens / Q000378:metabolism*; D018791:CD4 Lymphocyte Count; D002648:Child; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D005786:Gene Expression Regulation; D006262:Health; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism*; D053699:Interleukin-7 Receptor alpha Subunit / Q000378:metabolism*; D008297:Male; D051379:Mice; D008875:Middle Aged; D051381:Rats; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000378:metabolism*