title: [The degrading mechanism of collagen promoted by RGDS in hepatic stellate cell].
abstract: OBJECTIVE
  To study the effect of Arg-Gly-Asp-Ser (RGDS) tetrapeptide on the expression of matrix metalloproteinase-13 (MMP-13) and the tissue inhibitor of metalloproteinase-1(TIMP-1) in hepatic stellate cell(HSC) stimulated by fibronectin (FN).
  
  
  METHODS
  The expression of MMP-13 was evaluated by RT-PCR. TIMP-1 was analyzed by in-situ hybridization at mRNA level and by Western blot at protein level.
  
  
  RESULTS
  Treated by RGDS tetrapeptide for 2 h, the mRNA expression of MMP-13 was upregulated but the expression of TIMP-1 mRNA and its protein in HSC was inhibited.
  
  
  CONCLUSIONS
  The expression of MMP-13 can be induced by RGDS tetrapeptide and the expression of TIMP-1 can be inhibited by RGDS tetrapeptide, which is one of the mechanisms of its anti-fibrogenesis.
authors.affiliation: Department of Gastroenterology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China. loraliu@yeah.net
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Hui-qing
authors.initials: HQ
authors.lastname: Jiang
authors.forename: Xiao-lan
authors.initials: XL
authors.lastname: Zhang
authors.forename: Dong-qiang
authors.initials: DQ
authors.lastname: Zhao
authors.forename: Mei
authors.initials: M
authors.lastname: Song
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005353:Fibronectins / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D055166:Hepatic Stellate Cells / Q000187:drug effects* / Q000378:metabolism*; D053509:Matrix Metalloproteinase 13 / Q000235:genetics; D009842:Oligopeptides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism