Mycoplasma hyopneumoniae bacterins and porcine circovirus type 2 (PCV2) infection: induction of postweaning multisystemic wasting syndrome (PMWS) in the gnotobiotic swine model of PCV2-associated disease.
Groups (5 to 15 per group) of gnotobiotic swine were infected oronasally with porcine circovirus type 2 (PCV2) at 3 days of age and then given 1 of 6 different commercial Mycoplasma hyopneumoniae (M. hyopneumoniae) bacterins as either a single dose (7 d of age, 1 application products) or 2 doses (7 and 21 d of age, 2 application product). Control groups received PCV2 alone (n = 9) or were infected with PCV2 and immunized twice with keyhole limpet hemocyanin (KLH) emulsified in incomplete Freund's adjuvant (ICFA) (n = 7). Five of 7 (71%) PCV2-infected piglets immunized with KLH/ICFA developed mild or overt PMWS, whereas none of 9 piglets infected with PCV2 alone developed PMWS. Five of 12 (42%) piglets vaccinated with a commercial bacterin containing mineral oil adjuvant developed PMWS following vaccination. None of the PCV2-infected piglets in the other bacterin-vaccinated groups developed PMWS in this model of PCV2-associated disease. This difference in prevalence of PMWS in piglets given the mineral oil-adjuvanted M. hyopneumoniae bacterin and the other M. hyopneumoniae bacterin vaccination groups was statistically significant (P < 0.05).
Krakowka
Steven
S
Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Western College of Veterinary Medicine, Columbus, USA. krakowka.1@osu.edu
Ellis
John
J
McNeilly
Francis
F
Waldner
Cheryl
C
Rings
D Michael
DM
Allan
Gordon
G
D000276:Adjuvants, Immunologic; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D017925:Circovirus / Q000472:pathogenicity; D004195:Disease Models, Animal; D005856:Germ-Free Life; D006433:Hemocyanins; D007150:Immunohistochemistry / Q000662:veterinary; D045705:Mycoplasma hyopneumoniae / Q000276:immunology*; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000453:epidemiology* / Q000473:pathology / Q000517:prevention & control*; D011897:Random Allocation; D012720:Severity of Illness Index; D013552:Swine