A turn-on fluorescent indicator for citrate with micromolar sensitivity.
A turn-on fluorescent indicator for citric acid (citrate) has been developed, displaying high emission enhancement (+1500%) and low interference by other carboxylates. The sensor is based on the non-emissive copper(II) complex of a fluorescent amino amide, which, upon addition of citrate decomplexates to yield the emissive ligand. The detection limit estimated for this new chemosensing system is about 0.5 microM. This novel approach to the analysis of citrate constitutes an alternative ca. 10(2)-10(3) times more sensitive than the standard method based on the enzyme citrate lyase.
Burguete
M Isabel
MI
Departamento de Química Inorgánica y Orgánica, Unidad Asociada de Materiales Orgánicos Avanzados, Escuela Superior de Tecnología y Ciencias Experimentales, Universitat Jaume I-CSIC, Avda. Sos Baynat, s/n, E-12071, Castellón, Spain.
Galindo
Francisco
F
Luis
Santiago V
SV
Vigara
Laura
L
D002951:Citrates / Q000032:analysis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009097:Multienzyme Complexes / Q000737:chemistry; D007652:Oxo-Acid-Lyases / Q000737:chemistry; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared