Silencing of poly(ADP-ribose) polymerase in plants alters abiotic stress signal transduction.
Transgenic plants with reduced poly(ADP-ribose) polymerase (PARP) levels have broad-spectrum stress-resistant phenotypes. Both Arabidopsis thaliana and oilseed rape (Brassica napus) lines overexpressing RNA interference-PARP constructs were more resistant to various abiotic stress treatments in laboratory and greenhouse experiments without negative effects on growth, development, and seed production. This outperforming stress tolerance was initially attributed solely to a maintained energy homeostasis due to reduced NAD(+) consumption. We show that in PARP2-deficient Arabidopsis plants, the observed abiotic stress resistance can also be explained by alterations in abscisic acid levels that facilitate the induction of a wide set of defense-related genes.
Vanderauwera
Sandy
S
Department of Plant Systems Biology, Flanders Institute for Biotechnology (VIB), Technologiepark 927, 9052 Ghent, Belgium.
De Block
Marc
M
Van de Steene
Nancy
N
van de Cotte
Brigitte
B
Metzlaff
Michael
M
Van Breusegem
Frank
F
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D036563:Cyclic ADP-Ribose / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D046228:Microarray Analysis; D008954:Models, Biological; D018384:Oxidative Stress; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D015398:Signal Transduction / Q000235:genetics; D014158:Transcription, Genetic