Human protein-protein interaction networks and the value for drug discovery.
Systematic genome-wide and pathway-specific protein-protein interaction screens have generated a putative, organizing framework of the spatial interconnectivity of a large number of human proteins, including numerous therapeutically relevant disease-associated proteins. The intrinsic value for drug discovery is that these physical protein-protein interaction networks may contribute to a mechanistic understanding of the pathophysiology of disease and can aid in the identification and prioritization of tractable targets and generate hypotheses on how to best drug non-tractable, disease-associated targets. Here, we review the 'therapeutic potential' of the 1st generation sub-genome-scale human interaction networks and disease-associated protein networks generated by yeast two-hybrid screens and affinity purification-mass spectrometry approaches.
Ruffner
Heinz
H
Cellzome AG, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
Bauer
Andreas
A
Bouwmeester
Tewis
T
D015195:Drug Design; D006801:Humans; D013058:Mass Spectrometry; D040081:Protein Array Analysis; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000378:metabolism*; D020798:Two-Hybrid System Techniques