Evaluation of early conception factor lateral flow test to determine nonpregnancy in dairy cattle.
The early conception factor (ECF) lateral flow test was evaluated for its ability to accurately determine nonpregnant status in dairy cattle. Results of 2 field trials involving 191 cows and 832 tests indicated the probability that a cow can be correctly diagnosed as nonpregnant by using the ECF test is only about 50%. Agreement of test results between milk and serum obtained from the same cow was 57.5%. The ECF test was not consistent in identifying nonpregnancy when the same cows were tested repeatedly over a period of 4 weeks. We conclude that the ECF lateral flow test does not accurately identify nonpregnancy in dairy cattle.
Ambrose
Divakar J
DJ
Alberta Agriculture Food, Suite 204, 7000-113 Street, Edmonton, Alberta T6H 5T6. divakar.ambrose@gov.ab.ca
Radke
Brian
B
Pitney
Phyllis A
PA
Goonewardene
Laksiri A
LA
D000818:Animals; D002417:Cattle / Q000097:blood / Q000502:physiology*; D018835:Chaperonin 10; D005260:Female; D008297:Male; D008892:Milk / Q000737:chemistry; D010455:Peptides / Q000032:analysis*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis*; D011259:Pregnancy Tests, Immunologic / Q000379:methods / Q000662:veterinary*; D011270:Pregnancy, Animal / Q000097:blood* / Q000502:physiology; D011374:Progesterone / Q000097:blood; D011933:Reagent Kits, Diagnostic / Q000662:veterinary*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013491:Suppressor Factors, Immunologic / Q000032:analysis*