title: Floating culture promotes the maintenance of hematopoietic stem cells.
abstract: In this study we examined the effect of the specific gravity of culture medium on the frequency of hematopoietic stem cell (HSC) maintenance. We used a newly developed high-specific-gravity media. Bone marrow cells were isolated and cultured, and HSC activity was evaluated. The number of hematopoietic progenitor/stem cells was markedly higher in the medium with high specific gravity. In high-specific-gravity media, cells did not precipitate, maintenance of HSCs was increased, and there was a concomitant accumulation of beta-catenin. This novel technique for maintaining HSC populations provides an important new tool for studies in regenerative medicine.
authors.affiliation: Department of Pediatrics, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan. kajiume@hiroshima-u.ac.jp
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Kajiume
authors.forename: Louis
authors.initials: L
authors.lastname: Yuge
authors.forename: Yumi
authors.initials: Y
authors.lastname: Kawahara
authors.forename: Reiko
authors.initials: R
authors.lastname: Yoshimoto
authors.forename: Akira
authors.initials: A
authors.lastname: Sasaki
authors.forename: Toshinori
authors.initials: T
authors.lastname: Ide
authors.forename: Makoto
authors.initials: M
authors.lastname: Asashima
authors.forename: Katsuko
authors.initials: K
authors.lastname: Kataoka
authors.forename: Masao
authors.initials: M
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D003470:Culture Media; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D013997:Time Factors; D051176:beta Catenin / Q000378:metabolism