title: Fully automated sleep deprivation in mice as a tool in sleep research.
abstract: Although total sleep deprivation is frequently used in sleep research, the techniques used such as gentle handling are labor consuming and not standardized (and boring). In order to minimize these limitations, we developed a fully automated setup, which can be used for total sleep deprivation. A shortfall of individually adjustable thresholds of electromyogram (EMG) signals from sleep deprived animals was used online to switch running wheels incorporated into the home cages. Randomized direction of rotations, adaptable rotational speed and automatic deactivation of the running wheels during quiet waking of the animals provided robust and standardized sleep deprivation without increased stress, when compared to gentle handling. The setup can easily be introduced to a variety of home cages and is individually adaptable to each animal to be sleep deprived.
authors.affiliation: Max Planck Institute of Psychiatry, Kraepelinstrasse 2-10, 80804 Munich, Germany. tomf@mpipsykl.mpg.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Fenzl
authors.forename: Christoph P N
authors.initials: CP
authors.lastname: Romanowski
authors.forename: Cornelia
authors.initials: C
authors.lastname: Flachskamm
authors.forename: Karlheinz
authors.initials: K
authors.lastname: Honsberg
authors.forename: Erwin
authors.initials: E
authors.lastname: Boll
authors.forename: Arnold
authors.initials: A
authors.lastname: Hoehne
authors.forename: Mayumi
authors.initials: M
authors.lastname: Kimura
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004569:Electroencephalography / Q000379:methods; D004576:Electromyography / Q000379:methods; D004864:Equipment and Supplies; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000502:physiology; D012890:Sleep / Q000502:physiology*; D012892:Sleep Deprivation / Q000503:physiopathology*