Multipotent mesenchymal stem cells from adult human eye conjunctiva stromal cells.
Identification of mesenchymal stem cells (MSCs) derived from alternative sources has provided an exciting prospect for intensive investigation. This work focused on characterizing a new source of MSCs from stromal cells from human eye conjunctiva. In this study, after conjunctiva biopsies and culture of stromal segment of this tissue, fibroblast-like (SH2+, SH3+, CD29+, CD44+, CD166+, CD13+) human stromal cells, which can be differentiated toward the osteogenic, adipogenic, chondrogenic, and neurogenic lineages, were obtained. These cells expressed Oct-4, Nanog, Rex-1 genes, and some lineage-specific markers like cardiac actin and Keratin. Taken together, the results indicate that conjunctiva stromal-derived cells are a new source of multipotent MSCs and despite originating from an adult source, they express undifferentiated stem cell markers.
Nadri
Samad
S
Stem Cell Department, Stem Cell Technology Co. Ltd, Tehran, Iran.
Soleimani
Masoud
M
Kiani
Jafar
J
Atashi
Amir
A
Izadpanah
Reza
R
D000293:Adolescent; D000328:Adult; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D003228:Conjunctiva / Q000166:cytology*; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology* / Q000276:immunology