Disruption of intraflagellar transport in adult mice leads to obesity and slow-onset cystic kidney disease.
The assembly of primary cilia is dependent on intraflagellar transport (IFT), which mediates the bidirectional movement of proteins between the base and tip of the cilium. In mice, congenic mutations disrupting genes required for IFT (e.g., Tg737 or the IFT kinesin Kif3a) are embryonic lethal, whereas kidney-specific disruption of IFT results in severe, rapidly progressing cystic pathology. Although the function of primary cilia in most tissues is unknown, in the kidney they are mechanosenstive organelles that detect fluid flow through the tubule lumen. The loss of this flow-induced signaling pathway is thought to be a major contributing factor to cyst formation. Recent data also suggest that there is a connection between ciliary dysfunction and obesity as evidenced by the discovery that proteins associated with human obesity syndromes such as Alström and Bardet-Biedl localize to this organelle. To more directly assess the importance of cilia in postnatal life, we utilized conditional alleles of two ciliogenic genes (Tg737 and Kif3a) to systemically induce cilia loss in adults. Surprisingly, the cystic kidney pathology in these mutants is dependent on the time at which cilia loss was induced, suggesting that cyst formation is not simply caused by impaired mechanosensation. In addition to the cystic pathology, the conditional cilia mutant mice become obese, are hyperphagic, and have elevated levels of serum insulin, glucose, and leptin. We further defined where in the body cilia are required for normal energy homeostasis by disrupting cilia on neurons throughout the central nervous system and on pro-opiomelanocortin-expressing cells in the hypothalamus, both of which resulted in obesity. These data establish that neuronal cilia function in a pathway regulating satiety responses.
Davenport
James R
JR
Department of Cell Biology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
Watts
Amanda J
AJ
Roper
Venus C
VC
Croyle
Mandy J
MJ
van Groen
Thomas
T
Wyss
J Michael
JM
Nagy
Tim R
TR
Kesterson
Robert A
RA
Yoder
Bradley K
BK
D050154:Adiposity; D000818:Animals; D002923:Cilia / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005407:Flagella / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D052177:Kidney Diseases, Cystic / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D016547:Kinesins / Q000235:genetics; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D040542:Mechanotransduction, Cellular; D051379:Mice; D009154:Mutation; D009765:Obesity / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D010179:Pancreas / Q000378:metabolism / Q000473:pathology; D021381:Protein Transport / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics