title: [Effects of fumonisin on HLA-I expression on human peripheral blood mononuclear cells in vitro].
abstract: OBJECTIVE
  To study the effects of Fumonisin B1(FB1) on HLA-I on human peripheral blood mononuclear cells (PBMC) in vitro.
  
  
  METHODS
  The expression of HLA-I on PBMC by FB1 pretreatment at different dosage(10, 50 micromol/L) was detected with flow cytometry (FCM), Western blot, and semi-RT-PCR, respectively.
  
  
  RESULTS
  The expression of HLA-I on PBMC in vitro at the two experimental concentration was both lower than that of the control after FB1 treatment for 24 h as represented by fluorescence intensity by FCM analysis. Western blot results further confirmed the above results. At mRNA level, HLA-A, B and C mRNA were detected by RT-PCR, and the results showed that no changes were found on the expression of HLA-A, B mRNA between FB1 treated group and the control group, but HLA-C mRNA was inhibited in FB1 treated groups.
  
  
  CONCLUSIONS
  10 and 50 micromol/L FB1 could inhibit the expression of HLA-I on human PBMC in vitro at 24 h treatment.
authors.affiliation: Lab of Experimental Pathology, Hebei Medical University, Shijiazhuang 050017, China. xinglingxiao@hebmu.edu.cn
authors.forename: Ling-xiao
authors.initials: LX
authors.lastname: Xing
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Shen
authors.forename: Yue-hong
authors.initials: YH
authors.lastname: Li
authors.forename: Jun-ling
authors.initials: JL
authors.lastname: Wang
authors.forename: Xia
authors.initials: X
authors.lastname: Yan
authors.forename: Feng-rong
authors.initials: FR
authors.lastname: Wang
authors.forename: Xiang-hong
authors.initials: XH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005434:Flow Cytometry; D037341:Fumonisins / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction