CLIP170 autoinhibition mimics intermolecular interactions with p150Glued or EB1.
CLIP170 and p150(Glued) localize to the plus ends of growing microtubules. Using crystallography and NMR, we show that autoinhibitory interactions within CLIP170 use the same binding determinants as CLIP170's intermolecular interactions with p150(Glued). These interactions have both similar and distinct features when compared with the p150(Glued)-EB1 complex. Our data thus demonstrate that regulation of microtubule dynamics by plus end-tracking proteins (+TIPs) occurs through direct competition between homologous binding interfaces.
Hayashi
Ikuko
I
Division of Signaling Biology, Ontario Cancer Institute, University of Toronto, 101 College Street, Toronto, Ontario, M5G 1L7, Canada. ihayashi@uhnres.utoronto.ca
Plevin
Michael J
MJ
Ikura
Mitsuhiko
M
D000595:Amino Acid Sequence; D000818:Animals; D000072159:Dynactin Complex; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D016415:Sequence Alignment