title: Orally administered lactoperoxidase increases expression of the FK506 binding protein 5 gene in epithelial cells of the small intestine of mice: a DNA microarray study.
abstract: Lactoperoxidase (LPO) is a component of milk and other external secretions. To study the influence of ingested LPO on the digestive tract, we performed DNA microarray analysis of the small intestine of mice administered LPO. LPO administration upregulated 78 genes, including genes involved in metabolism, immunity, apoptosis, and the cell cycle, and downregulated nine genes, including immunity-related genes. The most upregulated gene was FK506 binding protein 5 (FKBP5), a glucocorticoid regulating immunophilin. The upregulation of this gene was confirmed by quantitative RT-PCR in other samples. In situ hybridization revealed that expression of the FKBP5 gene in the crypt epithelial cells of the small intestine was enhanced by LPO. These results suggest that ingested LPO modulates gene expression in the small intestine and especially increases FKBP5 gene expression in the epithelial cells of the intestine.
authors.affiliation: Food Science & Technology Institute, Morinaga Milk Industry Co., Ltd, Zama, Kanagawa 228-8583, Japan. h_wakaby@morinagamilk.co.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Wakabayashi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Miyauchi
authors.forename: Kouichirou
authors.initials: K
authors.lastname: Shin
authors.forename: Koji
authors.initials: K
authors.lastname: Yamauchi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Matsumoto
authors.forename: Keiko
authors.initials: K
authors.lastname: Abe
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Takase
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002417:Cattle; D002460:Cell Line; D015536:Down-Regulation; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism*; D007784:Lactoperoxidase / Q000008:administration & dosage / Q000494:pharmacology*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D022021:Tacrolimus Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D015854:Up-Regulation; D000099159:Tacrolimus Binding Protein 5