title: Introduction of a 50 kbp DNA fragment into the plastid genome.
abstract: Plastid transformation technology has been used for the analysis and improvement of plastid metabolism. To create a transplastomic plant with a complicated and massive metabolic pathway, it is necessary to introduce a large amount of DNA into the plastid. However, to our knowledge, the largest DNA fragment introduced into a plastid genome was only 7 kbp long and consisted of just three genes. Here we report the introduction of foreign DNA of 23-50 kbp into the tobacco plastid genome with a bacterial artificial chromosome (BAC)-based plastid transformation vector. It was confirmed that the introduced DNA was passed on to the next generation. This is the first description of plastid transformation with a large amount of foreign DNA.
authors.affiliation: Research Institute of Innovative Technology for the Earth, 9-2 Kizugawadai, Kizugawa-shi, Jyoto 619-0292, Japan. tadachi@tiger.kobe-u.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Adachi
authors.forename: Hisabumi
authors.initials: H
authors.lastname: Takase
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Tomizawa
mesh_terms: D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics*; D054627:Genome, Plastid / Q000235:genetics*; D030821:Plants, Genetically Modified; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic