Validation of a "new" short tandem repeat (STR) fluorescent multiplex system and report of population genetic data.
The Humantype Chimera PCR Amplification Kit contains 12 polymorphic loci (ACTBP2 (= SE33), D18S51, D4S2366, D6S474, D8S1132, D12S391, D2S1360, D3S1744, D5S2500, D7S1517, D10S2325, D21S2055), of which the latter 10 loci have not been used extensively for human identity testing. The sex determinant locus amelogenin is also included in the kit. Amplification was successful on a variety of thermal cyclers and the amplicons could be analyzed on both the ABI PRISM 310 and 3100 Genetic Analyzers. Complete genotyping results from single source samples were possible between 0.25 and 2 ng of DNA template. Heterozygote imbalance (< 60% peak height balance) caused by stochastic effects was observed at a rate of around 5%. No deviations from the Hardy-Weinberg equilibrium were observed. Thus, there were no detectable significant deviations from the expected genetic independence of alleles.
Henke
Lotte
L
Institut für Blutgruppenforschung LGC GmbH, Köln, Germany.
Muche
Matthias
M
Blaauw
André
A
Van Eede
Peter H
PH
Martin
Wolfgang
W
Helmken
Claudia
C
Budowle
Bruce
B
Henke
Juergen
J
D005060:Europe; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population / Q000295:instrumentation* / Q000379:methods*; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D010334:Paternity; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D044465:White People