The effects of murine cytomegalovirus on the maturation, fertilization, cleavage and blastula formation of mouse oocytes in vitro.
To study the effects of mouse cytomegalovirus (MCMV) on the in vitro maturation, fertilization, cleavage and blastula formation of mouse oocytes, the immature oocytes were infected in vitro by MCMVs of different dosages (100 TCID(50), 10 TCID(50) and 1 TCID(50)). The oocytes were then observed for in vitro maturation, fertilization, cleavage and blastula formation and the ultrastructural changes after the culture with the viruses. Our results showed that no significant differences were found in IVM, IVF, cleavage and blastula formation among the groups treated with of virus of various dosages. And ultrastructural abnormality was observed in the oocytes treated by 100 TCID(50) of viruses. It is concluded that MCMV did not have any conspicuous effects on IVM, IVF, cleavage and blastula formation of murine immature oocytes.
Wang
Xinrong
X
Department of Gynecology & Obstetrics, Huazhong University of Science and Technology, Wuhan 430030, China. csh@tjh.tjmu.edu.cn
Zhang
Xinhong
X
Chen
Suhua
S
Zhu
Guijin
G
Ai
Jihui
J
D000818:Animals; D001755:Blastocyst; D002478:Cells, Cultured; D002970:Cleavage Stage, Ovum; D003586:Cytomegalovirus Infections; D005260:Female; D005306:Fertilization; D066298:In Vitro Techniques; D051379:Mice; D018146:Muromegalovirus / Q000472:pathogenicity*; D009865:Oocytes / Q000166:cytology / Q000254:growth & development / Q000821:virology*