Effects of acute alcohol intoxication in the second trimester of pregnancy on development of the murine fetal lung.
OBJECTIVE
We hypothesized that administration of alcohol during the second trimester of gestation at the pseudoglandular phase of lung development might lead to aberrant differentiation and growth, similar to that seen in congenital cystic adenomatoid malformation in human. We further hypothesized that these effects would be apparent morphologically and by altered Hoxb5 expression.


METHODS
C57BL/6J mice, exposed to ethanol at embryonic day (E) 11.5 to E13.5, which corresponds to the pseudoglandular stage of lung development, were examined at E18.5. The lungs were analyzed histologically by immunostaining.


RESULTS
The average body and lung weight of alcohol-exposed (AE) fetuses were lower than those of control fetuses, the reduction in lung mass being more than the body weight. Histology showed that AE lungs were less developed and exhibited higher expression of Hoxb5 in AE lungs than controls.


CONCLUSIONS
Alcohol exposure at E13.5 affected fetal lung development, with delayed differentiation and increased Hoxb5.
Wang
Xiangyuan
X
Department of Obstetrics and Gynecology, College of Physicians and Surgeons, Columbia University, New York, NY, USA. lvb1@columbia.edu <lvb1@columbia.edu>
Gomutputra
Prasra
P
Wolgemuth
Debra J
DJ
Baxi
Laxmi
L
D000014:Abnormalities, Drug-Induced / Q000196:embryology* / Q000209:etiology; D000435:Alcoholic Intoxication / Q000150:complications*; D000818:Animals; D004195:Disease Models, Animal; D000431:Ethanol / Q000009:adverse effects / Q000494:pharmacology / Q000633:toxicity; D005260:Female; D047109:Fetal Development / Q000187:drug effects / Q000235:genetics; D005325:Fetal Organ Maturity / Q000187:drug effects* / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics; D008168:Lung / Q000002:abnormalities* / Q000187:drug effects / Q000196:embryology; D051379:Mice; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D049188:Prenatal Injuries / Q000139:chemically induced