SlyA and H-NS regulate transcription of the Escherichia coli K5 capsule gene cluster, and expression of slyA in Escherichia coli is temperature-dependent, positively autoregulated, and independent of H-NS.
In this paper, we present the first evidence of a role for the transcriptional regulator SlyA in the regulation of transcription of the Escherichia coli K5 capsule gene cluster and demonstrate, using a combination of reporter gene fusions, DNase I footprinting, and electrophoretic mobility shift assays, the dependence of transcription on the functional interplay between H-NS and SlyA. Both SlyA and H-NS bind to multiple overlapping sites within the promoter in vitro, but their binding is not mutually exclusive, resulting in a remodeled nucleoprotein complex. In addition, we show that expression of the E. coli slyA gene is temperature-regulated, positively autoregulated, and independent of H-NS.
Corbett
David
D
Faculty of Life Sciences, University of Manchester, Smith Bldg., Oxford Rd., Manchester M13 9PT, United Kingdom.
Bennett
Hayley J
HJ
Faculty of Life Sciences, University of Manchester, Smith Bldg., Oxford Rd., Manchester M13 9PT, United Kingdom.
Askar
Hamdia
H
Faculty of Life Sciences, University of Manchester, Smith Bldg., Oxford Rd., Manchester M13 9PT, United Kingdom; Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Egypt.
Green
Jeffrey
J
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom.
Roberts
Ian S
IS
Faculty of Life Sciences, University of Manchester, Smith Bldg., Oxford Rd., Manchester M13 9PT, United Kingdom. Electronic address: i.s.roberts@manchester.ac.uk.
D001426:Bacterial Proteins / Q000378:metabolism*; D001667:Binding, Competitive; D053938:DNA Fragmentation; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D008954:Models, Biological; D008957:Models, Genetic; D005810:Multigene Family; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013696:Temperature; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism*