[The study on the chromosome aneuploidy in human lung cancer].
OBJECTIVE
To study the feasibility of interphase fluorescence in situ hybridization (FISH) in detection of chromosomal aneuploidy in touch preparations of lung carcinoma and its clinic significance.


METHODS
We examined 46 touch preparations of lung carcinomas by FISH with DNA specific for chromosomes 7, 8, 9 and 12 to detect chromosomal aneuploidy.


RESULTS
Aneuploidy, mainly featured in gains of chromosomes 7, 8 and 12 and loss of chromosome 9 were observed in all of the touch preparations of the 46 cases with lung cancer. The rates of chromosomal aneuploidy detected by FISH were 67.4% (31/46), 60.9% (28/46), 28.3% (13/46) and 56.5% (26/31) respectively.


CONCLUSIONS
Interphase FISH is feasible in detecting aneuploidy in touch preparations of lung cancer. These alterations may be applied to early diagnosis and predicting prognosis of lung cancer.
Gao
Shu-geng
SG
Department of Thoracic Surgery, Cancer Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100021, China.
Dong
Xiang-yang
XY
Wang
Liang-jun
LJ
He
Jie
J
D000782:Aneuploidy; D018288:Carcinoma, Small Cell / Q000235:genetics / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D002881:Chromosomes, Human, Pair 12; D002897:Chromosomes, Human, Pair 7; D002898:Chromosomes, Human, Pair 8; D002899:Chromosomes, Human, Pair 9; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D009367:Neoplasm Staging; D015203:Reproducibility of Results