The genome of epsilon15, a serotype-converting, Group E1 Salmonella enterica-specific bacteriophage.
The genome sequence of the Salmonella enterica serovar Anatum-specific, serotype-converting bacteriophage epsilon15 has been completed. The nonredundant genome contains 39,671 bp and 51 putative genes. It most closely resembles the genome of phiV10, an Escherichia coli O157:H7-specific temperate phage, with which it shares 36 related genes. More distant relatives include the Burkholderia cepacia-specific phage, BcepC6B (8 similar genes), the Bordetella bronchiseptica-specific phage, BPP-1 (8 similar genes) and the Photobacterium profundum prophage, P Pphipr1 (6 similar genes). epsilon15 gene identifications based on homologies with known gene families include the terminase small and large subunits, integrase, endolysin, two holins, two DNA methylase enzymes (one adenine-specific and one cytosine-specific) and a RecT-like enzyme. Genes identified experimentally include those coding for the serotype conversion proteins, the tail fiber, the major capsid protein and the major repressor. epsilon15's attP site and the Salmonella attB site with which it interacts during lysogenization have also been determined.
Kropinski
Andrew M
AM
Department of Microbiology and Immunology, Queens University, Kingston, Ontario, Canada K7L 3N6.
Kovalyova
Irina V
IV
Billington
Stephen J
SJ
Patrick
Aaron N
AN
Butts
Brent D
BD
Guichard
Jared A
JA
Pitcher
Trevor J
TJ
Guthrie
Carly C
CC
Sydlaske
Anya D
AD
Barnhill
Lisa M
LM
Havens
Kyle A
KA
Day
Kenneth R
KR
Falk
Darrel R
DR
McConnell
Michael R
MR
D000595:Amino Acid Sequence; D001434:Bacteriophage Typing; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D012483:Salmonella Phages / Q000145:classification / Q000235:genetics* / Q000502:physiology; D019779:Salmonella enterica / Q000145:classification / Q000235:genetics / Q000821:virology*; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping; D013045:Species Specificity; D014764:Viral Proteins / Q000235:genetics; D019065:Virus Assembly; D016662:Virus Integration