Differential chain-length specificities of two isoamylase-type starch-debranching enzymes from developing seeds of kidney bean.
Plant isoamylase-type starch-debranching enzymes (ISAs) hydrolyze alpha-1,6-linkages in alpha-1,4/alpha-1,6-linked polyglucans. Two ISAs, designated PvISA1/2 and PvISA3, were purified from developing seeds of kidney bean by ammonium sulfate fractionation and several column chromatographic procedures. The enzymes displayed different substrate specificities for polyglucans: PvISA1/2 showed broad chain-length specificities, whereas PvISA3 liberated specific chains with a DP of 2 to 4.
Takashima
Yoshinori
Y
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
Senoura
Takeshi
T
Yoshizaki
Takayuki
T
Hamada
Shigeki
S
Ito
Hiroyuki
H
Matsui
Hirokazu
H
D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D007517:Isoamylase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D027805:Phaseolus / Q000201:enzymology* / Q000235:genetics; D012639:Seeds / Q000201:enzymology / Q000235:genetics; D013213:Starch / Q000378:metabolism*; D013379:Substrate Specificity; D014158:Transcription, Genetic / Q000235:genetics