Floating culture promotes the maintenance of hematopoietic stem cells.
In this study we examined the effect of the specific gravity of culture medium on the frequency of hematopoietic stem cell (HSC) maintenance. We used a newly developed high-specific-gravity media. Bone marrow cells were isolated and cultured, and HSC activity was evaluated. The number of hematopoietic progenitor/stem cells was markedly higher in the medium with high specific gravity. In high-specific-gravity media, cells did not precipitate, maintenance of HSCs was increased, and there was a concomitant accumulation of beta-catenin. This novel technique for maintaining HSC populations provides an important new tool for studies in regenerative medicine.
Kajiume
Teruyuki
T
Department of Pediatrics, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan. kajiume@hiroshima-u.ac.jp
Yuge
Louis
L
Kawahara
Yumi
Y
Yoshimoto
Reiko
R
Sasaki
Akira
A
Ide
Toshinori
T
Asashima
Makoto
M
Kataoka
Katsuko
K
Kobayashi
Masao
M
D000818:Animals; D016026:Bone Marrow Transplantation; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D003470:Culture Media; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D013997:Time Factors; D051176:beta Catenin / Q000378:metabolism