Bleomycin-induced double-strand breaks in mitochondrial DNA of Drosophila cells are repaired.
Mitochondrial DNA lesions cause numerous human diseases, and it is therefore important to identify the mechanisms whereby the mitochondrion repairs the damage. We have studied in cultured Drosophila cells the repair of bleomycin-induced double-strand breaks (DSBs) in mitochondrial DNA. Our results show that DSBs are repaired as rapidly and effectively in the mitochondria as in the nucleus. DNA repair is complete within 2h following bleomycin treatment, showing that Drosophila mitochondria have an effective system of DSB repair. The mechanism and mitochondrial proteins involved remain to be identified.
Morel
Frederic
F
Equipe Genome Mitochondrial, UMR CNRS 6547, Université Blaise-Pascal, Clermont 2, 63177, Aubière-Cedex, France.
Renoux
Monique
M
Lachaume
Philippe
P
Alziari
Serge
S
D000818:Animals; D001761:Bleomycin / Q000633:toxicity*; D004249:DNA Damage; D004260:DNA Repair; D004272:DNA, Mitochondrial / Q000187:drug effects*; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*