title: EMR1, the human homolog of F4/80, is an eosinophil-specific receptor.
abstract: The EGF-TM7 F4/80 is a defining marker of murine macrophage populations. Applying flow cytometric analysis using the newly generated mAb A10, and quantitative real-time PCR, we here report the surprising observation that the human ortholog of F4/80, EGF-like module containing mucin-like hormone receptor (EMR)1, is absent on mononuclear phagocytic cells including monocytes, macrophages, and myeloid dendritic cells. Unexpectedly, we found that EMR1 expression is restricted to eosinophilic granulocytes, where expression is overlapping with the eotaxin receptor CCR3 and the immunoglobulin-like lectin Siglec-8. Absence on other leukocytes, including basophils, implies that EMR1 is a highly specific marker for eosinophils in humans.
authors.affiliation: Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. j.hamann@amc.uva.nl
authors.forename: Jörg
authors.initials: J
authors.lastname: Hamann
authors.forename: Nathalie
authors.initials: N
authors.lastname: Koning
authors.forename: Walter
authors.initials: W
authors.lastname: Pouwels
authors.forename: Laurien H
authors.initials: LH
authors.lastname: Ulfman
authors.forename: Marco
authors.initials: M
authors.lastname: van Eijk
authors.forename: Martin
authors.initials: M
authors.lastname: Stacey
authors.forename: Hsi-Hsien
authors.initials: HH
authors.lastname: Lin
authors.forename: Siamon
authors.initials: S
authors.lastname: Gordon
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Kwakkenbos
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000943:Antigens, Differentiation / Q000737:chemistry / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D016466:CHO Cells; D002135:Calcium-Binding Proteins; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004804:Eosinophils / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D008253:Macaca mulatta; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009077:Mucins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002679:Pan troglodytes; D010215:Papio; D011148:Pongo pygmaeus; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012452:Saguinus; D040681:Structural Homology, Protein