Comparison of measurements of canine plasma glucose, creatinine, urea, total proteins, alanine aminotransferase, and alkaline phosphatase obtained with the APOLOWAKO and Vitros 250 analyzers.
The APOLOWAKO is an entirely automatic benchtop biochemistry analyzer that uses stabilized liquid reagents. It was tested for canine blood and plasma glucose, creatinine, urea, total proteins, alanine aminotransferase, and alkaline phosphatase. The APOLOWAKO gave very similar results for whole blood and the corresponding plasma (n=32). Within-laboratory imprecision was below 2.2% and 5.8% for substrates and enzymes, respectively. Comparison of results with whole blood by APOLOWAKO and with the corresponding plasma by Vitros 250 (n=139) showed very good correlations. Passing-Bablok's regression slopes ranged from 0.83 to 1.12 and intercepts were close to zero, except for ALP where the results obtained by APOLOWAKO were approximately 1.5 times higher than by Vitros. The APOLOWAKO system can be a reliable instrument in veterinary practices where larger systems are not available but it should be further validated and reference intervals should be determined.
Geffré
A
A
Département des Sciences cliniques, Ecole Nationale Vétérinaire, 23 Chemin des Capelles, 31076 Toulouse Cedex, France.
Braun
J P
JP
Germain
C
C
Palanché
F
F
Kueper
R
R
Trumel
C
C
D000410:Alanine Transaminase / Q000097:blood*; D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D001798:Blood Proteins / Q000032:analysis*; D003404:Creatinine / Q000097:blood*; D004285:Dogs; D015203:Reproducibility of Results; D014508:Urea / Q000097:blood*