Study of low-molecular weight organic acids in maize roots under the stress of cadmium using capillary zone electrophoresis.
An indirect CE method was developed for the study of low-molecular weight organic acids (LMWOAs) in maize under the stress of cadmium. The influences of indirect reagents (phthalate, salicylic acid, and benzoic acid), coion concentration, and pH were studied. A buffer composed of 15 mmol/L benzoate and 0.2 mmol/L CTAB (pH 5.7) was used in the organic acid determination. The detection limit ranged between 0.5 and 6 micromol/L. The RSD (n = 14) of the method was found to be in the range of 0.11-0.49% for migration time and 1.25-4.72% for peak area. In the maize roots, obvious increases of LMWOAs were observed when the plants were under the stress of cadmium and/or organic acids. The recovery of standard organic acids added in real samples ranged from 85 to 116%.
Guo
Bao-Yuan
BY
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
Peng
Zhen-Lei
ZL
Han
Fang
F
Shan
Xiao-Quan
XQ
Lin
Jin-Ming
JM
D000143:Acids / Q000032:analysis*; D002104:Cadmium / Q000633:toxicity*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D009930:Organic Chemicals / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D003313:Zea mays / Q000737:chemistry*