A reality check for alignments and trees.
Making multiple sequence alignments is one of the more commonplace procedures in modern biology. Multiple alignments are typically generated by feeding sequences into the alignment program from the N-terminus to the C-terminus. Recent results show that if the same sequences are processed from the C- to the N-terminus, a different alignment is often obtained. Because phylogenetic trees are built from alignments, the resulting trees can also differ. The new findings highlight sequence alignment as a crucial step in molecular evolutionary studies and provide straightforward measures to assess alignment reliability.
Martin
William
W
Institute of Botany III, University of Düsseldorf, Düsseldorf, Germany. w.martin@uni-duesseldorf.de
Roettger
Mayo
M
Lockhart
Peter J
PJ
D000595:Amino Acid Sequence; D000818:Animals; D019143:Evolution, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein