Cytotoxicity of triterpenes isolated from Aceriphyllum rossii.
Bioassay-guided fractionation of a MeOH extract of the whole plant of Aceriphyllum rossii (Saxifragaceae) led to the isolation of two new triterpenes, 3alpha,23-isopropylidenedioxyolean-12-en-27-oic acid (1) and 23-hydroxy-3-oxoolean-12-en-27-oic acid (2), together with six known triterpenes, 3-oxoolean-12-en-27-oic acid (3), 3alpha-hydroxyolean-12-en-27-oic acid (4), beta-peltoboykinolic acid (5), aceriphyllic acid A (6), oleanolic acid (7), and gypsogenic acid (8). The structures of these compounds were elucidated on the basis of physicochemical and spectroscopic analyses. These compounds were evaluated for in vitro cytotoxicity against the K562 and HL-60 cell lines. Olean-12-en-27-oic acid derivatives (1-6) exhibited considerable cytotoxicity against K562 and HL-60 cell lines with IC(50) values ranging from 12.2 to 28.7 microM and from 12.1 to 25.8 microM, respectively.
Lee
Iksoo
I
College of Pharmacy, Chungnam National University, Daejeon, Korea.
Yoo
Jae Kuk
JK
Na
MinKyun
M
Min
Byung Sun
BS
Lee
Jongpill
J
Yun
Bong Sik
BS
Jin
Wenyi
W
Kim
Hongjin
H
Youn
Uijung
U
Chen
Quan Cheng
QC
Song
Kyung Sik
KS
Seong
Yeon Hee
YH
Bae
Kihwan
K
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000633:toxicity*; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry; D031995:Saxifragaceae / Q000737:chemistry*; D012997:Solvents; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013778:Tetrazolium Salts; D013844:Thiazoles; D014315:Triterpenes / Q000302:isolation & purification / Q000633:toxicity*