Selection of mimotopes of Bovine Viral Diarrhoea Virus using a solid-phase peptide library.
Bovine Viral Diarrhoea Virus (BVDV) is an important pathogen of cattle, causing important economical losses world-wide. In this study, an 8-mer solid-phase peptide library was screened with a neutralising monoclonal antibody 157 to generate mimotopes mimicking a conformational neutralising epitope of BVDV E2 protein. Two sequences selected 157A1 LFEQYYYF and 157A2 LYRFGEFD that did not show a high structural or sequence similarity with BVDV E2 glycoprotein reacted specifically with monoclonal antibody 157 when presented as solid-phase peptides in a SPOT scan assay. These results indicate that combinatorial peptide libraries can be used to identify potential mimotopes of conformational epitopes.
Kalaycioglu
Atila T
AT
Department of Pathology and Infectious Diseases, Royal Veterinary College, Royal College Street, London NW1 OTU, UK. Ataka161@hotmail.com
Russell
Peter H
PH
Howard
Colin R
CR
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000914:Antibodies, Viral / Q000378:metabolism; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D019151:Peptide Library; D010455:Peptides / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*