title: Neural MMP-28 expression precedes myelination during development and peripheral nerve repair.
abstract: Mammalian matrix metalloproteinase 28 (MMP-28) is expressed in several normal adult tissues, and during cutaneous wound healing. We show that, in frog and mouse embryos, MMP-28 is expressed predominantly throughout the nervous system. Xenopus expression increases during neurulation and remains elevated through early limb development where it is expressed in nerves. In the mouse, neural expression peaks at embryonic day (E) 14 but remains detectable through E17. During frog hindlimb regeneration XMMP-28 is not initially expressed in the regenerating nerves but is detectable before myelination. Following hindlimb denervation, XMMP-28 expression is detectable along regenerating nerves before myelination. In embryonic rat neuron-glial co-cultures, MMP-28 decreases after the initiation of myelination. Incubation of embryonic brain tissue with purified MMP-28 leads to the degradation of multiple myelin proteins. These results suggest that MMP-28 plays an evolutionarily conserved role in neural development and is likely to modulate the axonal-glial extracellular microenvironment.
authors.affiliation: Biotherapeutic Discovery Research, Lilly Research Laboratories, Eli Lilly and Co., Indianapolis, Indiana 46225, USA. wernerse@lilly.com
authors.forename: Sean R
authors.initials: SR
authors.lastname: Werner
authors.forename: Anthony L
authors.initials: AL
authors.lastname: Mescher
authors.forename: Anton W
authors.initials: AW
authors.lastname: Neff
authors.forename: Michael W
authors.initials: MW
authors.lastname: King
authors.forename: Shalini
authors.initials: S
authors.lastname: Chaturvedi
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Duffin
authors.forename: Mark W
authors.initials: MW
authors.lastname: Harty
authors.forename: Rosamund C
authors.initials: RC
authors.lastname: Smith
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D047108:Embryonic Development; D006614:Hindlimb / Q000294:innervation / Q000502:physiology; D020782:Matrix Metalloproteinases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D053505:Matrix Metalloproteinases, Secreted / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009185:Myelin Proteins / Q000302:isolation & purification / Q000378:metabolism; D009186:Myelin Sheath / Q000502:physiology*; D009416:Nerve Regeneration; D009420:Nervous System / Q000196:embryology* / Q000378:metabolism; D010525:Peripheral Nerves / Q000166:cytology / Q000196:embryology / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D012038:Regeneration; D016415:Sequence Alignment; D029867:Xenopus Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology / Q000378:metabolism*