[Effects of fumonisin on HLA-I expression on human peripheral blood mononuclear cells in vitro].
OBJECTIVE
To study the effects of Fumonisin B1(FB1) on HLA-I on human peripheral blood mononuclear cells (PBMC) in vitro.


METHODS
The expression of HLA-I on PBMC by FB1 pretreatment at different dosage(10, 50 micromol/L) was detected with flow cytometry (FCM), Western blot, and semi-RT-PCR, respectively.


RESULTS
The expression of HLA-I on PBMC in vitro at the two experimental concentration was both lower than that of the control after FB1 treatment for 24 h as represented by fluorescence intensity by FCM analysis. Western blot results further confirmed the above results. At mRNA level, HLA-A, B and C mRNA were detected by RT-PCR, and the results showed that no changes were found on the expression of HLA-A, B mRNA between FB1 treated group and the control group, but HLA-C mRNA was inhibited in FB1 treated groups.


CONCLUSIONS
10 and 50 micromol/L FB1 could inhibit the expression of HLA-I on human PBMC in vitro at 24 h treatment.
Xing
Ling-xiao
LX
Lab of Experimental Pathology, Hebei Medical University, Shijiazhuang 050017, China. xinglingxiao@hebmu.edu.cn
Shen
Hai-tao
HT
Li
Yue-hong
YH
Wang
Jun-ling
JL
Yan
Xia
X
Wang
Feng-rong
FR
Zhang
Xiang-hong
XH
D000818:Animals; D015153:Blotting, Western; D005434:Flow Cytometry; D037341:Fumonisins / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction