Assessment of rabbit spleen size using ultrasonography.
Assessment of spleen size using the ultrasonography has become a standard practice in human. However, the assessment is not established method in experimental animals. To establish the index to assess the spleen size using ultrasonography, we measured the cross-section image of rabbit spleen during endotoxin shock. The image of the cross-section was appeared as triangle, and the height of the triangular image was defined as the spleen index. This spleen index showed strong correlation with the spleen weight. In conclusion, this method is suitable for observation of changes in rabbit spleen size and may reduce the number of rabbit in the longitudinal studies.
Takeda
Yuji
Y
JIMRO Co., Ltd., Research Division, Takasaki, Gunma, Japan. takeday@jimro.co.jp
Asaoka
Hideyuki
H
Ueno
Masamichi
M
Jimma
Fumie
F
Hidaka
Mayumi
M
Shibusawa
Hiromu
H
Kaneda
Kenta
K
Saniabadi
Abbi R
AR
Hiraishi
Katsuya
K
Kashiwagi
Nobuhito
N
D000818:Animals; D009929:Organ Size; D011817:Rabbits / Q000033:anatomy & histology*; D013154:Spleen / Q000033:anatomy & histology* / Q000000981:diagnostic imaging*; D014463:Ultrasonography