Analysis of hemoglobin adducts of acrylamide and glycidamide by liquid chromatography-electrospray ionization tandem mass spectrometry, as exposure biomarkers in French population.
The determination of biomarkers of acrylamide exposure in humans from general French population by measurement of hemoglobin adduct levels of acrylamide (AA) and glycidamide (GA) is presented. The analytical procedure included modified Edman degradation and LC-ESI-MS/MS analysis of the final derivatives using deuterated internal standards. Method performances were evaluated in terms of linearity, precision, accuracy, and sensitivity. The method was firstly assessed on rat blood samples and then applied to the study of background adducts levels of AA and GA in 68 human hemoglobin samples, showing mean levels of 33 and 23 pmol/g globin for AA and GA adducts, respectively.
Chevolleau
Sylvie
S
UMR1089 Xénobiotiques, INRA-ENVT, BP 3, F-31931 Toulouse, France. schevoll@toulouse.inra.fr
Jacques
Carine
C
Canlet
Cécile
C
Tulliez
Jacques
J
Debrauwer
Laurent
L
D020106:Acrylamide / Q000737:chemistry*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004781:Environmental Exposure / Q000032:analysis; D004852:Epoxy Compounds / Q000737:chemistry*; D005260:Female; D005602:France; D006454:Hemoglobins / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D008297:Male; D008875:Middle Aged; D015394:Molecular Structure; D011634:Public Health / Q000379:methods; D051381:Rats; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods