Utilization of acorn fringe for ellagic acid production by Aspergillus oryzae and Endomyces fibuliger.
Conversion of acorn fringe extract into ellagic acid production by Aspergillus oryzae and Endomyces fibuliger were investigated. The results showed that ellagic acid production was maximized when co-fermentation of the two fungi was performed at 30 degrees C and pH 5.0 with 5.7 g/l of initial substrate concentration, which were close to the optimal values for both fungi to yield an appropriate consortium of hydrolytic enzymes. Meanwhile, it was found that the co-fermentation could compensate the deficiencies in the level of polyphenol oxidase activity from pure A. oryzae and the levels of ellagitannin acyl hydrolase and beta-glucosidase activities from pure E. fibuliger, resulting in. 0.91 g/l of biomass concentration containing 1.84 g/l of ellagic acid. The research not only demonstrates that the co-fermentation is an effective approach to utilize forest byproduct for ellagic acid production, but also provides more evidences for understanding evolution of ellagic acid production with enzymes actions, which is important for process control of ellagic acid production in industrial application.
Huang
Wen
W
Institute for Nanobiomedical Technology and Membrane Biology, State Key Lab of Biotherapy of Human Diseases, Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China. huangwen@iee.pku.edu.cn
Li
Zhenshan
Z
Niu
Hai
H
Li
Lulu
L
Lin
Wensheng
W
Yang
Jinshui
J
D001236:Aspergillus oryzae / Q000201:enzymology / Q000378:metabolism*; D004610:Ellagic Acid / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D047348:Hydrolyzable Tannins / Q000378:metabolism; D007700:Kinetics; D029963:Quercus / Q000378:metabolism*; D012442:Saccharomycopsis / Q000201:enzymology / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors