title: Surface-induced x-ray reflection visualization of membrane orientation and fusion into multibilayers.
abstract: The fusion of lipid membranes at the air-water interface has been detected with the use of x-ray reflection as a high-resolution, surface-sensitive technique. The rate of this fusion for dimyristoylphosphatidylcholine (DMPC) bilayers is the highest at 29 degrees C, which coincides with the chain-melting phase-transition temperature for the top membrane layers. After 6 hours of incubation a stack of at least ten surface-ordered membrane bilayers in equilibrium with the bulk vesicle suspension is formed. Such fusion is thus surface-catalyzed but not restricted to the first surface layer. The process involves partial membrane dehydration near the solution surface which decreases toward the bulk.
authors.forename: G
authors.initials: G
authors.lastname: Cevc
authors.forename: W
authors.initials: W
authors.lastname: Fenzl
authors.forename: L
authors.initials: L
authors.lastname: Sigl