[Effect of IL-4 on ABCG2 expression in human lung adenocarcinoma cell lines].
OBJECTIVE
To investigate the effect of IL-4 on the expression of ABCG2 in lung adencarcinoma cell lines.


METHODS
The ABCG2 mRNA and protein expression was determined by semi-quantitative RT-PCR and Western blot respectively.


RESULTS
The ABCG2 mRNA and protein was detectable in lung adencarcinoma cell lines A549 and SPC-A-1. But IL-4 stimulation did not significantly affect the ABCG2 mRNA and protein expression in lung adencarcinoma cell lines.


CONCLUSIONS
IL-4 does not regulate ABCG2 expression in human lung adencarcinoma cell lines.
Zhang
Yu-fei
YF
Department of Respiratory Disease, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. zhang72@fmmu.edu.cn
Qi
Hao-wen
HW
Zhao
Feng
F
Li
Zhao-feng
ZF
Guo
Li
L
Mu
De-guang
DG
Xu
Xing-xiang
XX
D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism*; D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000818:Animals; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism