title: Liquid chromatography tandem mass spectrometry method for simultaneous determination of metoprolol tartrate and ramipril in human plasma.
abstract: A simple, rapid, sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of metoprolol tartrate (MT) and ramipril, in human plasma. Both the drugs were extracted by liquid-liquid extraction with diethyl ether-dichloromethane (70:30, v/v). The chromatographic separation was performed on a reversed-phase C8 column with a mobile phase of 10 mM ammonium formate-methanol (3:97, v/v). The protonated analyte was quantitated in positive ionization by multiple reaction monitoring with a mass spectrometer. The method was validated over the concentration range of 5-500 ng/ml for metoprolol and ramipril in human plasma. The precursor to product ion transitions of m/z 268.0-103.10 and m/z 417.20-117.20 were used to measure metoprolol and ramipril, respectively.
authors.affiliation: Bioequivalence Study Centre, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India. mailveeran@yahoo.com
authors.forename: K Veeran
authors.initials: KV
authors.lastname: Gowda
authors.forename: Uttam
authors.initials: U
authors.lastname: Mandal
authors.forename: P
authors.initials: P
authors.lastname: Senthamil Selvan
authors.forename: W D
authors.initials: WD
authors.lastname: Sam Solomon
authors.forename: Animesh
authors.initials: A
authors.lastname: Ghosh
authors.forename: Amlan Kanti
authors.initials: AK
authors.lastname: Sarkar
authors.forename: Sangita
authors.initials: S
authors.lastname: Agarwal
authors.forename: T
authors.initials: T
authors.lastname: Nageswar Rao
authors.forename: Tapan Kumar
authors.initials: TK
authors.lastname: Pal
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008790:Metoprolol / Q000097:blood* / Q000737:chemistry; D015394:Molecular Structure; D017257:Ramipril / Q000097:blood* / Q000737:chemistry; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*