title: Altered expression of basement membrane-related molecules in rat brain pericyte, endothelial, and astrocyte cell lines after transforming growth factor-beta1 treatment.
abstract: The basement membrane at the blood-brain barrier (BBB) plays important roles in maintaining the structure and function of capillary vessels. The BBB is constructed from endothelial cells, astrocytes and pericytes, but their interactions in the formation or maintenance of basement membrane have not been established. Transforming growth factor-beta1 (TGF-beta1) is known to increase fibronectin in brain capillary basement membrane with deposition of beta-amyloid. We previously reported that the mRNA level of alpha-smooth muscle actin in a brain capillary pericyte cell line TR-PCT1 was increased by treatment with TGF-beta1. In this study, expression of mRNAs encoding basement membrane-related molecules in TR-PCT1, a rat endothelial cell line TR-BBB13, and a type 2 astrocyte cell line TR-AST4 was evaluated by RT-PCR. The effects of TGF-beta1 on expression of basement membrane-related genes in these cell lines were also examined. Fibronectin, MMP-9, tPA, TIMP-1, and PAI-l in TR-PCT1 were higher than in TR-BBB13 and TR-AST4. In TR-PCT1 treated with TGF-beta1, collagen type IV, PAI-1, and MMP-9 were increased, and TIMP-2 was reduced. The change in PAI-1 mRNA was faster than those in MMP-9, TIMP-2, collagen type IV mRNAs. These results suggest that pericytes may be key cells in the maintenance of the basement membrane at the BBB.
authors.affiliation: Department of Pharmaceutics, Kyoritsu University of Pharmacy, Tokyo, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Kose
authors.forename: Tomoko
authors.initials: T
authors.lastname: Asashima
authors.forename: Mariko
authors.initials: M
authors.lastname: Muta
authors.forename: Hisashi
authors.initials: H
authors.lastname: Iizasa
authors.forename: Yoshimichi
authors.initials: Y
authors.lastname: Sai
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Terasaki
authors.forename: Emi
authors.initials: E
authors.lastname: Nakashima
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D001485:Basement Membrane / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D001921:Brain / Q000166:cytology*; D001923:Brain Chemistry / Q000187:drug effects*; D002460:Cell Line; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects; D020286:Pericytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*