[Expression and activity analysis of human 67kD laminin receptor in Pichia pastoris].
To carry out the secretive expression of human 67 kD laminin receptor (67LR), recombinant expression plasmid pPIC9K-67LR was constructed by inserting of 67LR cDNA into yeast expression vector pPIC9K. The 67LR protein was expressed in Pichia pastoris after induced by methanol, and about 12.56 mg electrophoresis purity 67LR could be obtained after the purification of 1L culture using affinity chromatograph column. In vitro competitive binding assay showed that target protein has an excellent biological activity. The successful expression of 67LR has placed a solid foundation for the research on structure and functions of 67LR.
Lian
Ji-Qin
JQ
Department of Biochemistry and Molecular Biology, Third Military Medical University, Chongqing 400038, China.
Dai
Xu-Fang
XF
Gan
Li-Xia
LX
He
Feng-Tian
FT
D005822:Genetic Vectors; D006801:Humans; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D017474:Receptors, Laminin / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism