An improved method for single step purification of metagenomic DNA.
An improved method for purification of intact metagenomic DNA from soil has been developed using Q-Sepharose, which purified the DNA from phenolic and humic acid contaminants in a single step. The entire procedure for purification took only 45 min. A total of 81% of DNA was recovered after purification and there was 84% reduction in humic acid contents. The purified DNA was readily digested with restriction enzymes and can be further used for molecular applications.
Sharma
Pushpender K
PK
Department of Biotechnology, Panjab University, Chandigarh, 160014, India.
Capalash
Neena
N
Kaur
Jagdeep
J
D001709:Biotechnology / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D016678:Genome; D006812:Humic Substances; D012988:Soil Microbiology