title: Measurement of feline serum interleukin-5 level.
abstract: A bioassay was developed to measure feline interleukin-5 (IL-5). Human IL-5 receptor alpha chain transfected murine Ba/F3 cells (Ba/F3-IL-5R) showed feline IL-5-dependent proliferation in a dose-dependent manner. IL-5 levels in serum samples from 54 cats with suspected allergic dermatitis and from 11 control cats could be successfully measured using Ba/F3-IL-5R cells. The number of eosinophils in peripheral blood was not correlated with serum IL-5 level.
authors.affiliation: Department of Veterinary Internal Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Nakazato
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Momoi
authors.forename: Michiyo
authors.initials: M
authors.lastname: Kadoya
authors.forename: Toshiroh
authors.initials: T
authors.lastname: Iwasaki
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002415:Cats / Q000097:blood*; D002460:Cell Line; D049109:Cell Proliferation; D003872:Dermatitis / Q000097:blood / Q000662:veterinary; D015848:Interleukin-5 / Q000097:blood*; D051379:Mice; D053649:Receptors, Interleukin-5 / Q000235:genetics