title: In-situ engineering of cartilage repair: a pre-clinical in-vivo exploration of a novel system.
abstract: This investigation explores a new cartilage repair technique that uses a novel method to secure a non-woven multifilamentous scaffold in the defect site after microfracture. The hypothesis is that a scaffold provides a larger surface area for attachment and proliferation of the mesenchymal stem cells that migrate from the bone marrow. Two in-vivo studies were undertaken in an ovine model. The first study, which lasted for 8 weeks, aimed to compare the new technique with microfracture. Chondral defects, 7 mm in diameter, were created in both femoral medial condyles of five ewes. One defect was treated with the new technique while the contralateral knee was treated with microfracture alone. The results revealed that the quantity of repair tissue was significantly greater in the defects treated with the new system. The second study had two time points, 3 and 6 months, and used 13 ewes. In this study, both defects were treated with the new technique but one received additional subchondral drilling in order to stimulate extra tissue growth. The majority of the implants had good tissue induction, filling 50-100 per cent of the defect volume, while the compressive modulus of the repairs was in the range of 40-70 per cent of that for the surrounding cartilage. In addition, hyaline-like cartilage was seen in all the repairs which had the additional drilling of the subchondral bone.
authors.affiliation: Bioengineering Division, Academic Unit of Musculoskeletal Diseases, University of Leeds, Leeds, UK. b.b.seedhom@leeds.ac.uk
authors.forename: B B
authors.initials: BB
authors.lastname: Seedhom
authors.forename: Z-J
authors.initials: ZJ
authors.lastname: Luo
authors.forename: A J
authors.initials: AJ
authors.lastname: Goldsmith
authors.forename: T
authors.initials: T
authors.lastname: Toyoda
authors.forename: J C
authors.initials: JC
authors.lastname: Lorrison
authors.forename: L
authors.initials: L
authors.lastname: Guardamagna
mesh_terms: D000818:Animals; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D050724:Fractures, Cartilage / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*; D048091:Guided Tissue Regeneration / Q000295:instrumentation* / Q000379:methods; D019736:Prostheses and Implants; D012756:Sheep; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D016896:Treatment Outcome