title: Partial restoration of immunoglobulin production by cytokines in common variable immunodeficiency.
abstract: We describe a 26-year-old female referred to us because of recurrent bacterial pneumonia. Her immunoglobulin profile on admission was; IgG 1920 mg/l, IgA 60 mg/l, IgM 260 mg/l, IgD below 20 mg/l, IgE below 1 kU/l. Antinuclear antibodies, EBV VCA IgM, anti-parvovirus B19 IgM antibodies and hepatitis infection markers were all negative. Bone marrow aspiration revealed normal cellularity without abnormal cells, especially plasma cell proliferation. No rearrangement for IgH and TCR was observed as determined by Southern blot analysis. By the given data, a diagnosis of common variable immunodeficiency (CVID) was made. The genesis of this disease remained unclear. In this study, proliferation and immunoglobulin production with or without several stimulators were examined. Proliferation stimulated by PHA, Con-A, LPS, or IL-2 was decreased compared to that of healthy individuals. Immunoglobulin production after stimulation with several agents was quite low. Interestingly, however, IL-2 or IL-4 could increase IgM production on 6 days culture significantly. These results indicate that IL-2 or IL-4 possibly restore T cell responses to several antigens and induce B cell differentiation.
authors.affiliation: Department of Hematology, Kinki University School of Medicine, Osaka, Japan. ymaeda@med.kindai.ac.jp
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Maeda
authors.forename: Terufumi
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Hijikata
authors.forename: Miyako
authors.initials: M
authors.lastname: Tanaka
authors.forename: Chikara
authors.initials: C
authors.lastname: Hirase
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Takai
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Morita
authors.forename: Akihisa
authors.initials: A
authors.lastname: Kanamaru
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D017074:Common Variable Immunodeficiency / Q000150:complications / Q000175:diagnosis / Q000276:immunology*; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000187:drug effects / Q000378:metabolism*; D007376:Interleukin-2 / Q000378:metabolism* / Q000494:pharmacology; D015847:Interleukin-4 / Q000378:metabolism* / Q000494:pharmacology; D007564:Japan; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D011014:Pneumonia / Q000150:complications* / Q000276:immunology; D012008:Recurrence