title: Analysis of IL-1A(-889) and TNFA(-308) gene polymorphism in Brazilian patients with generalized aggressive periodontitis.
abstract: Generalized aggressive periodontitis (GAP) comprises a group of periodontal diseases characterized by the rapid destruction of periodontal tissues which affect young individuals who generally present no systemic disorders. Polymorphisms in the interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha) genes have been associated with an increased severity of chronic periodontitis. The objective of the present study was to evaluate the association between IL-1A (-889) and TNFA (-308) gene polymorphisms and GAP. One hundred nonsmoking subjects were selected, including 30 with GAP and 70 without periodontal disease. Gene polymorphisms were analyzed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. For IL-1 (-889), the frequency of genotype 1/1 was 54.3% in the control group and 56.7% in the study group. The frequency of genotype 1/2 was 37.1% in the control group and 40% in the study group. Genotype 2/2 was detected at a frequency of 8.6% and 3.3% in the control and study groups, respectively. For TNFA, genotype 1/1 was present in 68.6% of control subjects and in 80.0% of patients with GAP, while the frequency of genotype 1/2 was 27.1% in the control group and 20% in the study group. Genotype 2/2 was present in 4.3% of control subjects and was not detected in the study group. The frequencies of allele 1 and allele 2 of the IL-1A (-889) gene were 72.9% and 27.1%, respectively, in the control group and 76.7% and 23.3% in the GAP group. For the TNFA (-308) gene, the frequency of allele 1 was 82.15% in the control group and 90% in the study group, whereas the frequency of allele 2 was 17.85% in the control group and 10% in the study group. Statistical analysis revealed no significant difference in allele distribution for either gene between the two groups. No association was observed between GAP and IL-1A (-889) and TNFA (-308) gene polymorphisms in Brazilian patients.
authors.affiliation: Department of Periodontology, Faculty of Odontology, University of São Paulo, Brazil. nmfreita@usp.br
authors.forename: Nívea
authors.initials: N
authors.lastname: Maria de Freitas
authors.forename: Ana Vitória
authors.initials: AV
authors.lastname: Imbronito
authors.forename: Adriana Costa
authors.initials: AC
authors.lastname: Neves
authors.forename: Fábio Daumas
authors.initials: FD
authors.lastname: Nunes
authors.forename: Francisco Emilio
authors.initials: FE
authors.lastname: Pustiglioni
authors.forename: Roberto Fraga Moreira
authors.initials: RF
authors.lastname: Lotufo
mesh_terms: D000208:Acute Disease; D000328:Adult; D001938:Brazil; D016022:Case-Control Studies; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007155:Immunologic Factors; D053582:Interleukin-1alpha / Q000235:genetics*; D008297:Male; D017622:Periodontal Attachment Loss / Q000235:genetics* / Q000473:pathology; D010518:Periodontitis / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012463:Saliva / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D044465:White People