Expression and functional activity of pro-oxidants and antioxidants in murine heart exposed to acute hypobaric hypoxia.
Under hypobaric hypoxia, antioxidant defenses of the heart are stressed by the enhanced production of ROS. Mammalian heart acclimatizes to hypoxia through altered gene expression, which we studied in murine heart exposed to 10h of acute hypobaric hypoxia (AHH), equivalent to 15000ft, using cDNA arrays. Functional classification of genes with a > or =2-fold change revealed a number of pro-oxidants like Cyba, Xdh, Txnip, Ppp1r15b and antioxidants like Cat, Gpx1, Mt1, Mgst1. Interestingly, the protein level of Cyba, a subunit of NADPH oxidase, was markedly decreased in AHH exposed heart, suggesting the involvement of some stress response pathways. The AHH exposure also caused a significant reduction (50%) in the level of GSH (P<0.05). The present study provides a retrospective insight on the cellular antioxidant defense mechanisms under AHH.
Karar
Jayashree
J
Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India.
Dolt
Karamjit S
KS
Mishra
Manoj K
MK
Arif
Ehtesham
E
Javed
Saleem
S
Pasha
M A Qadar
MA
D000208:Acute Disease; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D051379:Mice; D009206:Myocardium / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction