title: Induction of immune responses in mice after intragastric administration of Lactobacillus casei producing porcine parvovirus VP2 protein.
abstract: Lactobacillus casei ATCC 393 was selected as an antigen delivery vehicle for mucosal immunization against porcine parvovirus (PPV) infection. A 64-kDa fragment of PPV major protective antigen VP2 protein was used as the parvovirus antigen model. A recombinant Lactobacillus expressing VP2 protein was constructed with plasmid pPG611.1, where expression and localization of the VP2 protein from recombinant Lc393-rPPV-VP2 was detected via sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blotting, and immunofluorescence. Both local mucosal and systemic immune responses against PPV were induced in BALB/c mice immunized orally with the recombinant Lactobacillus expressing VP2 protein. The induced antibodies demonstrated neutralizing effects on PPV infection. These data indicated that the use of recombinant lactobacilli could be a valuable strategy for future vaccine development of PPV.
authors.affiliation: Veterinary Department, Northeast Agricultural University, Harbin 150030, People's Republic of China.
authors.forename: Yigang
authors.initials: Y
authors.lastname: Xu
authors.forename: Yijing
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D004333:Drug Administration Routes; D016503:Drug Delivery Systems; D018928:Immunity, Mucosal / Q000187:drug effects* / Q000276:immunology; D007780:Lacticaseibacillus casei / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000378:metabolism