title: [Clinical significance of changes in quantitative expression of human leukocyte antigen DR in severely burned patients].
abstract: OBJECTIVE
  To investigate the clinical significance of kinetic changes in quantitative expression of human leukocyte antigen DR (HLA-DR) in severely burned patients.
  
  
  METHODS
  The blood samples of 77 extensively burned patients (>30% of total body surface area) were serially collected in the present study. The expression of HLA-DR on CD14(+) mononuclear cell surface in burned patients were quantified by flow cytometry (using monoclonal antibody, QuantiBRITETM Anti-HLA-DR PE(*)/Anti-Monocyte PerCP-Cy5.5) on days 1, 3, 5, 7, 14, 21 and 28 post burn.
  
  
  RESULTS
  The expressions of HLA-DR on CD14(+) mononuclear cell surface in severely burned patients were significantly lower than those in healthy volunteers from the first day post burn (P < 0.05), and the value of HLA-DR expression was negatively correlated with the burned area (r = -0.7232, P < 0.05). The expression of HLA-DR in patients complicated with multiple organ dysfunction syndrome (MODS) was persistently decreased following major burns, and it was significantly lower than that of non-MODS patients on days 3, 14, 21 and 28 post burn (P < 0.05). The incidence rate of MODS rose markedly along with the lowering of HLA-DR expression, accompanied with poorer prognosis.
  
  
  CONCLUSIONS
  Extensive burns could result in marked damage in expression of HLA-DR on CD14(+) mononuclear cell surface and immunologic dysfunction. Quantitative measurement of HLA-DR expression might be of significance in forecasting the development of MODS and prognosis in extensively burned patients.
authors.affiliation: Burns Institute, First Hospital Affiliated to the China People's Liberation Army General Hospital, Beijing 100037, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Dong
authors.forename: Yong-ming
authors.initials: YM
authors.lastname: Yao
authors.forename: Yu-jue
authors.initials: YJ
authors.lastname: Cao
authors.forename: Li-xin
authors.initials: LX
authors.lastname: He
authors.forename: Jia-ke
authors.initials: JK
authors.lastname: Chai
authors.forename: Shan
authors.initials: S
authors.lastname: Xu
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Sheng
mesh_terms: D000293:Adolescent; D000328:Adult; D002056:Burns / Q000097:blood* / Q000150:complications / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000097:blood*; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000097:blood; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D009102:Multiple Organ Failure / Q000097:blood / Q000209:etiology / Q000276:immunology; D011379:Prognosis