title: Amplification of vacuole-targeting fungicidal activity of antibacterial antibiotic polymyxin B by allicin, an allyl sulfur compound from garlic.
abstract: A cationic antibacterial peptide, polymyxin B (PMB), was evaluated as an antifungal antibiotic against various yeasts and filamentous fungi when used in combination with allicin, an allyl sulfur compound from garlic. Allicin was not lethal but could markedly amplify the fungicidal activity of PMB, which was weakly detected with the increase in the plasma membrane permeability in Saccharomyces cerevisiae. Their combined actions caused a dynamic structural damage to the yeast vacuole as judged by the disappearance of its swollen spherical architecture. The vacuole-targeting activity of PMB was similarly amplified in medium with t-butyl hydroperoxide as a substitute for the action of allicin. These findings suggest that the allicin-mediated lipoperoxide production in fungal plasma membrane is the cause of the enhancement in the cellular uptake of PMB as well as its action against the vacuole.
authors.affiliation: Research Center for Urban Health and Sports, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Ogita
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nagao
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Fujita
authors.forename: Toshio
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents; D001419:Bacteria / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004220:Disulfides; D004357:Drug Synergism; D005658:Fungi / Q000187:drug effects; D005737:Garlic / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D010539:Permeability; D011112:Polymyxin B / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism; D013379:Substrate Specificity; D013441:Sulfinic Acids / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D014617:Vacuoles / Q000378:metabolism; D020122:tert-Butylhydroperoxide