Determination of vigabatrin in human plasma by means of CE with LIF detection.
A method has been developed for the quantitation of the antiepileptic drug vigabatrin (VGB) in human plasma. It is based on CE with LIF detection. The effect of the pH of the buffer and of N-methylglucamine (GLC) as BGE constituent was investigated. The final BGE consisted of 50 mM borate buffer, pH 9.0, with 100 mM GLC and enabled separation within 12 min at 20 kV voltage. An SPE procedure was used for the pretreatment of biological samples, based on mixed-mode lipophilic-cation exchange cartridges, followed by a derivatization step with 6-carboxyfluorescein-N-succinimidyl ester (CFSE). Fluorescence was excited by an Ar-ion laser (lambda(exc) = 488 nm). Linearity was observed in the 10-120 microg/mL plasma concentration range. Extraction yield was >96%, precision (expressed as RSD) <6.7% and accuracy (recovery) was between 97.0 and 101.6%. The method has been successfully applied to the analysis of VGB in plasma of epileptic patients undergoing therapy with the drug.
Musenga
Alessandro
A
Pharmaco-Toxicological Analysis Laboratory, Department of Pharmaceutical Sciences, Alma Mater Studiorum-University of Bologna, Via Belmeloro 6, I-40126 Bologna, Italy.
Mandrioli
Roberto
R
Comin
Irene
I
Kenndler
Ernst
E
Raggi
Maria Augusta
MA
D000927:Anticonvulsants / Q000097:blood*; D016903:Drug Monitoring; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D005452:Fluoresceins; D005453:Fluorescence; D006801:Humans; D007834:Lasers; D015394:Molecular Structure; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013388:Succinimides; D020888:Vigabatrin / Q000097:blood*