Signaling adaptor protein Crk is indispensable for malignant feature of glioblastoma cell line KMG4.
Signaling adaptor protein Crk has been shown to be involved in pathogenesis of human cancers including brain tumor where Crk was reported to be overexpressed. In this study, we addressed whether Crk is indispensable for malignant phenotype of brain tumor. In 20 surgical specimens of glioma, mRNA of both CrkI and CrkII was found to be elevated in malignant tumor. To define a precise role of Crk, we have established Crk-knockdown cell lines of glioblastoma KMG4 by siRNA, and early phase of cell adhesion to laminin was found to be suppressed. Wound healing assay revealed the decreased cell motility in Crk knockdown cells, and suppression of both anchorage-dependent and -independent growth were demonstrated in these cells. Furthermore, in vivo tumor forming potential was also markedly suppressed. These results suggest that Crk is required for early attachment to laminin, cell motility, and growth of glioblastoma cell line KMG4.
Wang
Lei
L
Laboratory of Molecular and Cellular Pathology, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan, and Department of Obstetrics & Gynecology, First Affiliated Hospital of Chongqing University of Medical Sciences, China.
Tabu
Kouichi
K
Kimura
Taichi
T
Tsuda
Masumi
M
Linghu
Hua
H
Tanino
Mishie
M
Kaneko
Sadao
S
Nishihara
Hiroshi
H
Tanaka
Shinya
S
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism*; D050736:Oncogene Protein v-crk / Q000378:metabolism*