title: Influence of surgical preparation on the in-vivo response of osteochondral defects.
abstract: Cartilage has an extremely poor capacity to heal, which has lead to intensive research into biomaterials and tissue engineering for the purpose of regenerating cartilage in vivo. Many of these techniques have shown great promise in vitro; however, the results do not always carry across to the in-vivo scenario. Healthy cartilage autografts often do not integrate with the adjacent cartilage, suggesting that cartilage is rarely capable of healing even under ideal conditions. It is hypothesized in this study that the surgical creation of defects in cartilage causes significant damage to the adjacent tissues, leading to further degradation of the cartilage and poor outcome for the repair in general. This study compares the healing response of osteochondral defects created with either a punch or a drill in the weight-bearing region of the sheep knee at 4 and 26 weeks following surgery. The use of a drill to create the defect creates a more aggressive inflammatory response at 4 weeks compared with a punch. However, by 26 weeks, defects created with a punch scored higher on the O'Driscoll cartilage grading scale. Tissue damage at the time of surgery plays an important part in the sequence of events for healing of cartilage defects. This knowledge will help to characterize and refine the ovine model for cartilage regeneration and may have an influence on surgical technique and instrumentation for clinical cartilage repair.
authors.affiliation: Surgical and Orthopaedic Research Laboratories, University of New South Wales, Prince of Wales Hospital, Randwick, New South Wales, Australia.
authors.forename: F
authors.initials: F
authors.lastname: Vizesi
authors.forename: R
authors.initials: R
authors.lastname: Oliver
authors.forename: P
authors.initials: P
authors.lastname: Smitham
authors.forename: T
authors.initials: T
authors.lastname: Gothelf
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.forename: W R
authors.initials: WR
authors.lastname: Walsh
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000293:injuries / Q000473:pathology* / Q000503:physiopathology*; D004195:Disease Models, Animal; D050724:Fractures, Cartilage / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D012756:Sheep; D014945:Wound Healing / Q000502:physiology*