Cholinesterase inhibiting and antiplasmodial steroidal alkaloids from Sarcococca hookeriana.
Bioguided phytochemical investigation of Sarcococca hookeriana with respect to the cholinesterase enzyme inhibitory assay yielded two new pregnane-type steriodal alkaloids hookerianamide H (1) and hookerianamide I (2), along with three known alkaloids N(a)-methylepipachysamine D (3), sarcovagine C (4) and dictyophlebine (5). Their structures were determined with the aid of extensive spectroscopic analysis. All compounds showed good inhibitory activities against the enzymes acetylcholinesterase (IC(50) 2.9-34.1 microM) and butyrylcholinesterase (IC(50) 0.3-3.6 microM). These compounds also showed moderate antiplasmodial activity (IC(50) 2.4-10.3 microM) against the Plasmodium falciparum chloroquine resistant W2 strain.
Devkota
Krishna Prasad
KP
Department of Chemistry, Organic and Bioorganic Chemistry, Bielefeld University, Bielefeld, Germany. devkotakpd@yahoo.com
Lenta
Bruno Ndjakou
BN
Choudhary
Muhammad Iqbal
MI
Naz
Qamar
Q
Fekam
Fabrice Boyom
FB
Rosenthal
Philip Joseph
PJ
Sewald
Norbert
N
D000470:Alkaloids / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D000962:Antimalarials; D002091:Butyrylcholinesterase / Q000378:metabolism; D031234:Buxaceae / Q000737:chemistry*; D002800:Cholinesterase Inhibitors; D004351:Drug Resistance; D009682:Magnetic Resonance Spectroscopy; D010963:Plasmodium falciparum / Q000187:drug effects; D011278:Pregnanes / Q000302:isolation & purification / Q000494:pharmacology*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013256:Steroids / Q000302:isolation & purification* / Q000494:pharmacology*