title: Validation of a "new" short tandem repeat (STR) fluorescent multiplex system and report of population genetic data.
abstract: The Humantype Chimera PCR Amplification Kit contains 12 polymorphic loci (ACTBP2 (= SE33), D18S51, D4S2366, D6S474, D8S1132, D12S391, D2S1360, D3S1744, D5S2500, D7S1517, D10S2325, D21S2055), of which the latter 10 loci have not been used extensively for human identity testing. The sex determinant locus amelogenin is also included in the kit. Amplification was successful on a variety of thermal cyclers and the amplicons could be analyzed on both the ABI PRISM 310 and 3100 Genetic Analyzers. Complete genotyping results from single source samples were possible between 0.25 and 2 ng of DNA template. Heterozygote imbalance (< 60% peak height balance) caused by stochastic effects was observed at a rate of around 5%. No deviations from the Hardy-Weinberg equilibrium were observed. Thus, there were no detectable significant deviations from the expected genetic independence of alleles.
authors.affiliation: Institut für Blutgruppenforschung LGC GmbH, Köln, Germany.
authors.forename: Lotte
authors.initials: L
authors.lastname: Henke
authors.forename: Matthias
authors.initials: M
authors.lastname: Muche
authors.forename: André
authors.initials: A
authors.lastname: Blaauw
authors.forename: Peter H
authors.initials: PH
authors.lastname: Van Eede
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Martin
authors.forename: Claudia
authors.initials: C
authors.lastname: Helmken
authors.forename: Bruce
authors.initials: B
authors.lastname: Budowle
authors.forename: Juergen
authors.initials: J
authors.lastname: Henke
mesh_terms: D005060:Europe; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population / Q000295:instrumentation* / Q000379:methods*; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D010334:Paternity; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D044465:White People