title: Lysine decarboxylase-negative Salmonella enterica serovar enteritidis: antibiotic susceptibility, phage and PFGE typing.
abstract: One hundred twenty Salmonella Enteritidis isolates collected from 1992 to 2005 in Nagasaki prefecture (65 isolates from 40 outbreak cases, 44 from sporadic diarrhea patients, and 11 from chicken-related products) were investigated by their antibiotic susceptibility profiles, phage typing, and pulsed-field gel electrophoresis (PFGE) typing. Out of them, 18 were identified as lysine decarboxylase (LDC)-negative isolates, and 15 showed resistance toward streptomycin. Based on the PFGE typing, the isolates were classified into five clusters by UPGMA clustering method. Three LDC-negative isolates belonged to cluster A and were of phage type (PT) 4 and isolated between 2000 and 2004. Other 15 LDC-negative isolates belonged to cluster E. They were PT1, reacted but did not conform (RDNC), or untypable and were isolated between 2001 and 2004. LDC-negative isolates of the cluster A differed from LDC-negative isolates of the cluster E in antibiotic susceptibility profiles, phage typing, and PFGE typing. LDC-negative isolates of the cluster E were isolated after 2001 in Nagasaki prefecture.
authors.affiliation: Nagasaki Prefectural Institute for Environmental Research and Public Health, Ikeda, Omura, Nagasaki, Japan. syamasaki@pref.nagasaki.lg.jp
authors.forename: Shogo
authors.initials: S
authors.lastname: Yamasaki
authors.forename: Kenshi
authors.initials: K
authors.lastname: Hara
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Izumiya
authors.forename: Haruo
authors.initials: H
authors.lastname: Watanabe
authors.forename: Naoaki
authors.initials: N
authors.lastname: Misawa
authors.forename: Karoku
authors.initials: K
authors.lastname: Okamoto
authors.forename: Kozo
authors.initials: K
authors.lastname: Takase
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001434:Bacteriophage Typing; D002262:Carboxy-Lyases / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D007564:Japan / Q000453:epidemiology; D010802:Phylogeny; D012480:Salmonella Infections / Q000453:epidemiology / Q000382:microbiology; D012477:Salmonella enteritidis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*