Construction of a yeast strain with regulatable phospholipid synthesis for analysis of the uptake and metabolism of phosphatidylethanolamine with short acyl chains.
We constructed a yeast stain, TKY12Ga, in which phosphatidylethanolamine (PE) synthesis can be controlled by means of the carbon source in the medium. When PE synthesis was blocked, its growth was inhibited. However, in the presence of exogenous didecanoyl PE (diC10PE), TKY12Ga grew despite an inability to synthesize PE. Our system, which employs TKY12Ga strain and diC10PE, provides a valuable tool to study the transport and metabolism of PE in yeast.
Deng
Lan
L
Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Kakihara
Toru
T
Fukuda
Ryouichi
R
Ohta
Akinori
A
D009154:Mutation / Q000235:genetics; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism