[Studies on purification and properties of antagonistic protein from bacteria SS02 of Paenibacillus daejeonensis].
The antifungal, anti-bacterical, anti-brine shrimp activities of SD22 isolated from Paenibacillus daejeonensis Bacteria SS02 were studied. The separation steps included ultracentrifugation, ultrafiltration and (NH4)2SO4 fractional precipitation, further purification was performed by SephadexG-75 and DEAE-32 chromatography. Its molecular weight determined by SDS-PAGE was 56.0 kD and its isoelectfic point was 6.4. SD22 was thermostable to some extent and stable to ultraviolet, but sensitive to some of the enzyme. SD22 could kill most pathogens from propagation, such as Rhizoctonia cerealis, Sclerotinia sclerotiorum Physalospora piricala, Trichodema viride, Gliocladium viride, Curvularia leaf spot, Fusarium sp, Fusarium head blight, Beauveria Bassiana, Escherichia coli, Staphylococcus aureus, Bacillus subtilis , Candidal vaginitis, Fusarium oxysporum Schl. emend. Sayder & Hansem et al. The results will be helpful to find out a novel antifungal protein.
Zhu
Kai
K
College of Life Sciences, Sichuan Normal University, Chengdu 610068, China.
Zhang
Xiao-Yu
XY
Ren
Zhi
Z
Feng
Ding-Sheng
DS
Wang
Yi-Ding
YD
D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects; D001407:Bacillus / Q000737:chemistry* / Q000235:genetics; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000494:pharmacology*; D020462:Gliocladium / Q000187:drug effects; D010944:Plants / Q000382:microbiology; D012232:Rhizoctonia / Q000187:drug effects