title: The effect of reduced dose and number of treatments of FSH on superovulatory response in CIDR-treated Korean native cows.
abstract: The objective of this study was to investigate the effect of dosage and number of days of follicle stimulating hormone (FSH) treatment on superovulatory response in controlled internal drug release (CIDR)-treated Korean native cows. Forty cows underwent two superovulatory treatments with a crossover design. Cows, at random stages of the estrous cycle, received a CIDR together with injections of 1 mg estradiol benzoate and 50 mg progesterone, and gonadotropin treatment began 4 days later. The cows were divided into 2 groups based on the dosage and number of days of treatment with porcine FSH; a total of 28 mg FSH was given in twice daily intramascular injections in decreasing doses over 4 days (5, 5, 4, 4, 3, 3, 2 and 2 mg; T1 group, n=20) or a total of 24 mg FSH was given in twice daily decreasing doses over 3 days (5, 5, 4, 4, 3 and 3 mg; T2 group, n=20). This was followed by the alternate treatment in the subsequent superovulation. The cows were treated identically in all other respects. PGF(2alpha) (25 mg and 15 mg) was given with the 5th and 6th injections of FSH, CIDR were withdrawn at the 6th FSH injection and the cows received 200 microg GnRH 36 h after CIDR withdrawal. The cows were artificially inseminated twice, at 48 and 60 h after CIDR withdrawal, using commercial semen from four Korean native bulls, and embryos were recovered 6 or 7 days after the 2nd insemination. The numbers of corpora lutea (CL; 7.9+/-1.0 vs. 8.3+/-1.1) and large follicles (1.2+/-0.2 vs. 1.3+/-0.3) present at the time embryo recovery, as detected by ultrasonography, did not differ between the T1 and T2 groups (P>0.05). Similarly, the numbers of total ova/embryos (6.2+/-0.9 vs. 6.4+/-1.1), transferable embryos (3.4+/-0.8 vs. 3.2+/-0.7), degenerate embryos (0.8+/-0.2 vs. 1.0+/-0.3) and unfertilized ova (2.1+/-0.5 vs. 2.2+/-0.5) did not differ between the groups (P>0.05). These data indicate that a reduced dose (24 vs. 28 mg) and number of treatments (6 vs. 8) of FSH for superovulation of CIDR-treated Korean native cows does not affect the embryo yield.
authors.affiliation: Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Korea. sonds@rda.go.kr
authors.forename: Dong-Soo
authors.initials: DS
authors.lastname: Son
authors.forename: Chang-Yong
authors.initials: CY
authors.lastname: Choe
authors.forename: Sang-Rae
authors.initials: SR
authors.lastname: Cho
authors.forename: Sun-Ho
authors.initials: SH
authors.lastname: Choi
authors.forename: Hyun-Jong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Ill-Hwa
authors.initials: IH
authors.lastname: Kim
mesh_terms: D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004343:Drug Implants; D004624:Embryo Transfer / Q000662:veterinary*; D030762:Estrous Cycle / Q000187:drug effects; D004973:Estrus Synchronization / Q000187:drug effects*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D007723:Korea; D013480:Superovulation / Q000187:drug effects*