Quantitative analysis of biofilm EPS uronic acid content.
The uronic acids assay was evaluated for its ability to measure the amount of uronic acids contained within a biofilm exopolysaccharide matrix. Cytophaga lytica, a marine bacterium isolated from a naturally occurring biofilm, was used to form single-species biofilms for the method assessment. The assay was found to be simple, reproducible, and sensitive to 1 microg levels, suggesting its potential for application as a screening technique for compounds that inhibit the production of microbial biofilm exopolysaccharide containing uronic acids.
Mojica
Kristina
K
Hawaii Natural Energy Institute, School of Ocean and Earth Sciences and Technology, University of Hawaii, Honolulu HI 96822, United States.
Elsey
Danielle
D
Cooney
Michael J
MJ
D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D001681:Biological Assay; D003589:Cytophaga / Q000254:growth & development / Q000502:physiology*; D005109:Extracellular Matrix / Q000737:chemistry*; D008386:Marine Biology; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism*; D014574:Uronic Acids / Q000378:metabolism*