Impedimetric immunosensor for the specific label free detection of ciprofloxacin antibiotic.
Impedance spectroscopy approaches combined with the immunosensor technology have been used for the determination of trace amounts of ciprofloxacin antibiotic belonging to the fluoroquinolone family. The sensor electrode was based on the immobilization of anti-ciprofloxacin antibodies by chemical binding onto a poly(pyrrole-NHS) film electrogenerated on a solid gold substrate. The electrode surface was modified by electropolymerization of pyrrole-NHS, antibody grafting and ciprofloxacin immunoreaction. The sensitive steps of surface modification, cyclic voltammetry (CV) and atomic force microscopy (AFM) imaging have been used for electrode surface characterization. The immunoreaction of ciprofloxacin on the grafted anti-ciprofloxacin antibody directly triggers a signal via impedance spectroscopy measurements which allows the detection of extremely low concentration of 10 pg/ml ciprofloxacin.
Ionescu
Rodica E
RE
Laboratoire des Sciences Analytiques, Université Claude Bernard Lyon I, 69622 Villeurbanne Cedex, France.
Jaffrezic-Renault
Nicole
N
Bouffier
Laurent
L
Gondran
Chantal
C
Cosnier
Serge
S
Pinacho
Daniel G
DG
Marco
M-Pilar
MP
Sánchez-Baeza
Francisco J
FJ
Healy
Thomas
T
Martelet
Claude
C
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002939:Ciprofloxacin / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D015203:Reproducibility of Results