Anticytomegaloviral activity of esterified milk proteins and L-polylysines.
MRC-5 fibroblasts infected with human cytomegalovirus (HCMV) reference strain AD 169 were treated with different concentrations of methylated alpha-lactalbumin (Met-ALA) or methylated beta-lactoglobulin (Met-BLG), as well as with their peptic hydrolysates, and with the highly basic polypeptides such as are L-polylysines (4-15 kDa). The antiviral activity was calculated by comparing the number of infected cells in the presence and absence of the tested substances. Both Met-ALA and Met-BLG, as well as their peptic hydrolysates, decreased the infectious activity of cytomegalovirus in fibroblast cells. As expected, L-polylysines showed the highest antiviral activity. However, the tested basic proteins and polypeptides despite their lower antiviral activities might be potentially quite useful in fight of arising drug resistance activities and the persistence capacities of this virus.
Chobert
Jean-Marc
JM
UR 1268 Biopolymères Interactions Assemblages, INRA, Equipe Fonctions et Interactions des Protéines Laitières, Nantes, France. chobert@nantes.inra.fr
Sitohy
Mahmoud
M
Billaudel
Sylviane
S
Dalgalarrondo
Michèle
M
Haertlé
Thomas
T
D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D003587:Cytomegalovirus / Q000187:drug effects*; D004951:Esterification; D005347:Fibroblasts / Q000187:drug effects / Q000821:virology; D006801:Humans; D007768:Lactalbumin / Q000737:chemistry / Q000494:pharmacology*; D007782:Lactoglobulins / Q000737:chemistry / Q000494:pharmacology*; D008745:Methylation; D008894:Milk Proteins / Q000737:chemistry / Q000494:pharmacology*; D011107:Polylysine / Q000737:chemistry / Q000494:pharmacology*