Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to Fasciola hepatica in milk.
Antibodies against Fasciola hepatica were detected in serum and individual milk samples of dairy cattle using an ELISA. Percentage positivity (PP) values in milk samples were related to serum PP values and were not influenced by days into lactation. The correlation coefficient between serum and individual milk samples was highly significant (r=0.84, P<0.005). The correlation coefficient between herd seroprevalence and herd milk antibody prevalence was 0.96. The correlation coefficient between prevalence measured by faecal egg count and both seroprevalence and milk antibody prevalence within the herd was 0.87. The diagnostic sensitivity and specificity for milk were 92% (95% CI=89-96) and 88% (95% CI=85-91), respectively, when the serum test was considered as a gold standard. In conclusion, the level of antibody to F. hepatica in milk is significantly correlated with the antibody level in serum and this ELISA is suitable as a means of routine veterinary diagnosis of exposure to F. hepatica in cattle and an alternative to testing sera.
Salimi-Bejestani
M R
MR
School of Veterinary Medicine, University of Semnan, PO Box 35195-363, Semnan, Iran. msalimi@semnan.ac.ir
Daniel
R
R
Cripps
P
P
Felstead
S
S
Williams
D J L
DJ
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis* / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D005260:Female; D007774:Lactation; D008892:Milk / Q000276:immunology*; D010270:Parasite Egg Count