title: Molecular cloning of an insertion sequence-like element from Vibrio anguillarum and its functional identification in E. coli.
abstract: The tdh (thermostable direct hemolysin) gene occurs in some strains of Vibrio species. All tdh genes are flanked by insertion sequence-like elements (ISV). All previous attempts have failed to detect transposition of these ISVs. In this work, we have built a transposition detection system in E. coli and succeeded in detecting the transposition of an insertion sequence-like element at a frequency of Km(r) mutants of 7.2 x 10(-6). A specific flanking sequence (5'-Py-Pu-3') was found on either side of the target duplication.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
authors.forename: Na
authors.initials: N
authors.lastname: Liang
authors.forename: Haizhen
authors.initials: H
authors.lastname: Wu
authors.forename: Jiang
authors.initials: J
authors.lastname: Ye
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.forename: Yuanxing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Huizhan
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D001427:Bacterial Toxins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D006460:Hemolysin Proteins / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D014733:Vibrio / Q000235:genetics*