Sequence analysis and expression of a blue-light photoreceptor gene, Le.phrA from the basidiomycetous mushroom Lentinula edodes.
We cloned and sequenced a photoreceptor gene (Le.phrA) from the basidiomycete Lentinula edodes. The product of Le.phrA, Le.PHRA (924 aa residues) contained a serine-rich region, an LOV domain and two PAS domains. It was clearly smaller than other fungal LOV domain-containing blue-light photoreceptors such as Coprinopsis cinerea Dst1 (1,175 aa), Neurospora crassa WC-1 (1,167 aa), and Cryptococcus neoformans WC-1 (1,141 aa). The Le.phrA gene was found to be transcribed at all stages of the fruiting-body formation of L. edodes, but it was most abundantly transcribed in the immature fruiting body. Fully-matured fruiting body also contained relatively large amounts of Le.phrA transcript. Although the transcript level was lower, preprimordial aggregated mycelial cells grown under a light environment contained larger amounts of the transcript than those grown under continuous darkness, suggesting a light-enhanced expression of the Le.phrA gene. Hymenophore-depleted pileus contained a markedly higher level of the transcript than the stipe.
Sano
Hiroaki
H
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan. sano.h.aa@m.titech.ac.jp
Narikiyo
Takatsugu
T
Kaneko
Shinya
S
Yamazaki
Takashi
T
Shishido
Kazuo
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003116:Color; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D008027:Light; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D020942:Shiitake Mushrooms / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics