Transport of beads by several kinesin motors.
The movements of beads pulled by several kinesin-1 (conventional kinesin) motors are studied both theoretically and experimentally. While the velocity is approximately independent of the number of motors pulling the beads, the walking distance or run-length is strongly increased when more motors are involved. Run-length distributions are measured for a wide range of motor concentrations and matched to theoretically calculated distributions using only two global fit parameters. In this way, the maximal number of motors pulling the beads is estimated to vary between two and seven motors for total kinesin concentrations between 0.1 and 2.5 microg/ml or between 0.27 and 6.7 nM. In the same concentration regime, the average number of pulling motors is found to lie between 1.1 and 3.2 motors.
Beeg
Janina
J
Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.
Klumpp
Stefan
S
Dimova
Rumiana
R
Gracià
Rubèn Serral
RS
Unger
Eberhard
E
Lipowsky
Reinhard
R
D000818:Animals; D001692:Biological Transport; D016547:Kinesins / Q000378:metabolism*; D008027:Light; D008863:Microspheres; D008870:Microtubules / Q000378:metabolism; D012542:Scattering, Radiation; D013552:Swine
D016547:Kinesins