title: Rapid determination of viral RNA sequences in mosquitoes collected in the field.
abstract: A method for rapid determination of viral RNA sequences (RDV) was applied to homogenates of Aedes aegypti collected in Thailand in an area in which dengue fever (dengue hemorrhagic fever) is endemic, using the mosquito cell line C6/36. Nucleic acid sequences of dengue virus type 4 and cell fusing agent virus were detected. This RDV method has the potential to become a standard method for detection of both known and newly emerging, unknown mosquito-borne viruses.
authors.affiliation: Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kihara
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Satho
authors.forename: Yuki
authors.initials: Y
authors.lastname: Eshita
authors.forename: Kouji
authors.initials: K
authors.lastname: Sakai
authors.forename: Akira
authors.initials: A
authors.lastname: Kotaki
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Takasaki
authors.forename: Yupha
authors.initials: Y
authors.lastname: Rongsriyam
authors.forename: Narumon
authors.initials: N
authors.lastname: Komalamisra
authors.forename: Raweewan
authors.initials: R
authors.lastname: Srisawat
authors.forename: Parichat
authors.initials: P
authors.lastname: Lapcharoen
authors.forename: Suchada
authors.initials: S
authors.lastname: Sumroiphon
authors.forename: Shiroh
authors.initials: S
authors.lastname: Iwanaga
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ushijima
authors.forename: Daiji
authors.initials: D
authors.lastname: Endoh
authors.forename: Takeshi
authors.initials: T
authors.lastname: Miyata
authors.forename: Akira
authors.initials: A
authors.lastname: Sakata
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Kashige
authors.forename: Fumio
authors.initials: F
authors.lastname: Miake
authors.forename: Shuetsu
authors.initials: S
authors.lastname: Fukushi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saijo
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morikawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D002460:Cell Line; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D019595:Severe Dengue / Q000821:virology; D013785:Thailand
chemical_list: D012367:RNA, Viral