title: Two members of the Arabidopsis CLC (chloride channel) family, AtCLCe and AtCLCf, are associated with thylakoid and Golgi membranes, respectively.
abstract: Though numerous pieces of evidence point to major physiological roles for anion channels in plants, progress in the understanding of their biological functions is limited by the small number of genes identified so far. Seven chloride channel (CLC) members could be identified in the Arabidopsis genome, amongst which AtCLCe and AtCLCf are both more closely related to bacterial CLCs than the other plant CLCs. It is shown here that AtCLCe is targeted to the thylakoid membranes in chloroplasts and, in agreement with this subcellular localization, that the clce mutants display a phenotype related to photosynthesis activity. The AtCLCf protein is localized in Golgi membranes and functionally complements the yeast gef1 mutant disrupted in the single CLC gene encoding a Golgi-associated protein.
authors.affiliation: Institut des Sciences du Végétal, Centre National de la Recherche Scientifique (CNRS-UPR 2355), Bât 22, avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Marmagne
authors.forename: Marion
authors.initials: M
authors.lastname: Vinauger-Douard
authors.forename: Dario
authors.initials: D
authors.lastname: Monachello
authors.forename: Andéol Falcon
authors.initials: AF
authors.lastname: de Longevialle
authors.forename: Céline
authors.initials: C
authors.lastname: Charon
authors.forename: Michèle
authors.initials: M
authors.lastname: Allot
authors.forename: Fabrice
authors.initials: F
authors.lastname: Rappaport
authors.forename: Francis-André
authors.initials: FA
authors.lastname: Wollman
authors.forename: Hélène
authors.initials: H
authors.lastname: Barbier-Brygoo
authors.forename: Geneviève
authors.initials: G
authors.lastname: Ephritikhine
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D015153:Blotting, Western; D018118:Chloride Channels / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D018745:Genome, Plant; D006056:Golgi Apparatus / Q000378:metabolism*; D010788:Photosynthesis; D013050:Spectrometry, Fluorescence; D020524:Thylakoids / Q000378:metabolism*
chemical_list: D018118:Chloride Channels