Gene expression profiling of the rat endometriosis model.
OBJECTIVE
To investigate the molecular mechanism of endometriosis, gene expression profiling was analyzed in a rat endometriosis model.


METHODS
An endometriosis model was induced by uterine autotransplantation in the peritoneal cavity on a female-SD rat (8 weeks old). As control samples, the normal uterine tissues were used. The gene expression was compared between endometriotic lesions and normal uterine tissues by cDNA microarray analysis, quantitative real time RT-PCR and immunohistochemistry.


RESULTS
The expression of 71 genes was upregulated and that of 45 genes was downregulated in the endometriotic lesions compared to normal uterine tissues. The upregulated genes included genes encoding cytokines, chemokines, growth factors and cell adhesion molecules. The levels of transcripts of osteopontin, Lyn, Vav1, Runx1, and l-selectin in the endometriotic lesions were 130, 10, 10, 12 and 46-fold higher than the respective levels in the eutopic endometrial samples.


CONCLUSIONS
The results suggest that osteopontin, Lyn, Vav1, Runx1, and l-selectin play important roles in the pathogenesis of endometriosis.
Konno
Ryo
R
Department of Gynecology, Omiya Medical Center Jichi Medical University, Amanuma-cho, Omiya, Saitama, Japan. kryo77@excite.co.jp
Fujiwara
Hiroyuki
H
Netsu
Sachiho
S
Odagiri
Kohei
K
Shimane
Miyuki
M
Nomura
Hitoshi
H
Suzuki
Mitsuaki
M
D000818:Animals; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D019041:L-Selectin / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D051567:Proto-Oncogene Proteins c-vav / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019061:src-Family Kinases / Q000235:genetics / Q000378:metabolism
D050676:Core Binding Factor Alpha 2 Subunit; D051567:Proto-Oncogene Proteins c-vav; D012333:RNA, Messenger; C493727:Runx1 protein, rat; C506897:Spp1 protein, rat; C496620:Vav1 protein, rat; D053495:Osteopontin; D019041:L-Selectin; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases