title: Distribution, functional expression, and genetic organization of Cif, a phage-encoded type III-secreted effector from enteropathogenic and enterohemorrhagic Escherichia coli.
abstract: Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) inject effector proteins into host cells via a type III secretion system encoded by the locus of enterocyte effacement (LEE). One of these effectors is Cif, encoded outside the LEE by a lambdoid prophage. In this study, we demonstrated that the Cif-encoding prophage of EPEC strain E22 is inducible and produces infectious phage particles. We investigated the distribution and functional expression of Cif in 5,049 E. coli strains of human, animal, and environmental origins. A total of 115 E. coli isolates from diverse origins and geographic locations carried cif. The presence of cif was tightly associated with the LEE, since all the cif-positive isolates were positive for the LEE. These results suggested that the Cif-encoding prophages have been widely disseminated within the natural population of E. coli but positively selected within the population of LEE-positive strains. Nonetheless, 66% of cif-positive E. coli strains did not induce a typical Cif-related phenotype in eukaryotic cells due to frameshift mutations or insertion of an IS element in the cif gene. The passenger region of the prophages carrying cif was highly variable and showed various combinations of IS elements and genes coding for other effectors such as nleB, nleC, nleH, nleG, espJ, and nleA/espI (some of which were also truncated). This diversity and the presence of nonfunctional effectors should be taken into account to assess EPEC and EHEC pathogenicity and tropism.
authors.affiliation: INRA, UMR 1225, Ecole Nationale Vétérinaire de Toulouse, Toulouse 31076, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Loukiadis
authors.forename: Rika
authors.initials: R
authors.lastname: Nobe
authors.forename: Sylvia
authors.initials: S
authors.lastname: Herold
authors.forename: Clara
authors.initials: C
authors.lastname: Tramuta
authors.forename: Yoshitoshi
authors.initials: Y
authors.lastname: Ogura
authors.forename: Tadasuke
authors.initials: T
authors.lastname: Ooka
authors.forename: Stefano
authors.initials: S
authors.lastname: Morabito
authors.forename: Monique
authors.initials: M
authors.lastname: Kérourédan
authors.forename: Hubert
authors.initials: H
authors.lastname: Brugère
authors.forename: Herbert
authors.initials: H
authors.lastname: Schmidt
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hayashi
authors.forename: Eric
authors.initials: E
authors.lastname: Oswald
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D054324:Enterohemorrhagic Escherichia coli / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology* / Q000821:virology; D054308:Enteropathogenic Escherichia coli / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology* / Q000821:virology; D029968:Escherichia coli Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D039002:Prophages / Q000235:genetics; D014774:Virulence
chemical_list: C481998:Cif protein, E coli; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins