Effect of peripheral axotomy on gene expression of NIDD in rat neural tissues.
Peripheral nerve lesion-induced production of neuronal nitric oxide synthase (nNOS) was implicated to influence a range of postaxotomy processes necessary for neuronal survival and nerve regeneration (Zochodne et al., Neuroscience, 91:1515-1527, 1999; Keilhoff et al., Journal of Chemical Neuroanatomy, 24:181-187, 2002, Nitric Oxide, 10:101-111, 2004). Protein-protein interactions represent an important mechanism in the control of NOS spatial distribution or activity (Alderton et al., Biochemical Journal, 357:593-615, 2001; Dedio et al., FASEB Journal, 15:79-89, 2001; Zimmermann et al., Proceedings of the National Academy of Sciences, 99:17167-17172, 2002). As one of the nNOS-binding proteins, nNOS-interacting DHHC domain-containing protein with dendritic mRNA (NIDD) has recently been identified to increase nNOS enzyme activity by targeting nNOS to the synaptic plasma membrane in a postsynaptic density protein 95/discs-large/zona occlusens-1 domain dependent manner (Saitoh et al., Journal of Biological Chemistry, 279:29461-29468, 2004). In this paper, we established a rat model with peripheral axotomy to investigate the gene expression patterns of NIDD in neural tissues using TaqMan quantitative real-time polymerase chain reaction and in situ hybridization combined with immunofluorescence. It revealed that NIDD mRNA was upregulated after sciatic nerve transection with the similar expressing styles as that of the nNOS in the injured nerves, corresponding dorsal root ganglia, and lumbar spinal cord. These findings imply that NIDD may be involved in the different pathological conditions including nerve regeneration, neuron loss or survival, and even pain process, possibly via regulating the enzyme nNOS activity.
Cheng
Chun
C
The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, People's Republic of China.
Chen
Mengling
M
Shi
Shuxian
S
Gao
Shangfeng
S
Niu
Shuqiong
S
Li
Xin
X
Liu
Haiou
H
Qin
Yongwei
Y
Shen
Aiguo
A
D000818:Animals; D019771:Axotomy; D002352:Carrier Proteins / Q000235:genetics*; D017931:DNA Primers; D005260:Female; D005786:Gene Expression Regulation; D015971:Gene Expression Regulation, Enzymologic; D007700:Kinetics; D008297:Male; D009474:Neurons / Q000201:enzymology / Q000502:physiology*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics*; D017933:Peripheral Nervous System / Q000502:physiology*; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000201:enzymology / Q000502:physiology; D001613:beta 2-Microglobulin / Q000235:genetics
D002352:Carrier Proteins; D017931:DNA Primers; C488668:Zdhhc23 protein, rat; D001613:beta 2-Microglobulin; D052247:Nitric Oxide Synthase Type II