title: The role of Hansenula polymorpha MIG1 homologues in catabolite repression and pexophagy.
abstract: In the methanol-utilizing yeast Hansenula polymorpha, glucose and ethanol trigger the repression of peroxisomal enzymes at the transcriptional level, and rapid and selective degradation of methanol-induced peroxisomes by means of a process termed pexophagy. In this report we demonstrate that deficiency in the putative H. polymorpha homologues of transcriptional repressors Mig1 (HpMig1 and HpMig2), as well as HpTup1, partially and differentially affects the repression of peroxisomal alcohol oxidase by sugars and ethanol. As reported earlier, deficiency in HpTup1 leads to impairment of glucose- or ethanol-induced macropexophagy. In H. polymorpha mig1mig2 double-deletion cells, macropexophagy was also substantially impaired, whereas micropexophagy became a dominant mode of autophagic degradation. Our findings suggest that homologues of the elements of the Saccharomyces cerevisiae main repression pathway have pleiotropic functions in H. polymorpha.
authors.affiliation: Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine.
authors.forename: Olena G
authors.initials: OG
authors.lastname: Stasyk
authors.forename: Tim
authors.initials: T
authors.lastname: van Zutphen
authors.forename: Huyn
authors.initials: H
authors.lastname: Ah Kang
authors.forename: Oleh V
authors.initials: OV
authors.lastname: Stasyk
authors.forename: Marten
authors.initials: M
authors.lastname: Veenhuis
authors.forename: Andriy A
authors.initials: AA
authors.lastname: Sibirny
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000595:Amino Acid Sequence; D050260:Carbohydrate Metabolism; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D000432:Methanol / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D008954:Models, Biological; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D020675:Peroxisomes / Q000378:metabolism* / Q000648:ultrastructure; D010843:Pichia / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*
chemical_list: D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D000432:Methanol