title: Breakthrough performance of large proteins on ion-exchange membrane columns.
abstract: Protein adsorption of large proteins on ion-exchange membrane columns was theoretically and experimentally investigated using batch and fixed-bed systems. Thyroglobulin was used as the model protein. The study strongly suggests that part of the protein is physically retained inside the column during frontal mode operation. These experimental results were used to obtain a filtration function of the chromatographic system. In the theoretical analysis of the frontal protein adsorption, a model was integrated by the serial coupling of the membrane-transport model, the filtration model and the system-dispersion model. Two different techniques were employed in the estimation of the maximum adsorption capacity, the equilibrium desorption constant and the forward interaction rate constant, which are the parameters of the membrane-transport model. The fit of the model to the experimental data was not possible using the equilibrium parameters obtained in the batch experiments. The parameter estimation using a simplex optimization routine coupled to the solution of the partial differential model equations yields full prediction of the adsorption phenomena.
authors.affiliation: Departamento de Biotecnología y Bioingeniería, CINVESTAV-IPN (Centro de Investigacion y de Estudios Avanzados-Instituto Politécnico Nacional), Mexico City, D.F. (Distrito Federal), CP 07360, Mexico.
authors.forename: Rosa Maria
authors.initials: RM
authors.lastname: Montesinos-Cisneros
authors.forename: Armando
authors.initials: A
authors.lastname: Lucero-Acuña
authors.forename: Jaime
authors.initials: J
authors.lastname: Ortega
authors.forename: Roberto
authors.initials: R
authors.lastname: Guzmán
authors.forename: Armando
authors.initials: A
authors.lastname: Tejeda-Mansir
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods*; D003198:Computer Simulation; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007475:Ion Exchange Resins / Q000737:chemistry*; D008567:Membranes, Artificial; D008956:Models, Chemical; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D007475:Ion Exchange Resins; D008567:Membranes, Artificial; D011506:Proteins