Reassessing the role of phosphocaveolin-1 in cell adhesion and migration.
Although phosphorylation on tyrosine 14 was identified early in the discovery of caveolin-1, the functional significance of this modification still remains elusive. Recent evidence points to a role of caveolin-1 tyrosine 14 phosphorylation in cell adhesion and migration. These results are based on a variety of tools, including a widely used mouse monoclonal anti-phosphocaveolin-1 antibody, which labels, in cultured cells, a protein localized at or near focal adhesions. We here report results from three independent laboratories, showing that this antibody recognizes phosphocaveolin-1 amongst other proteins in immunoblot analyses and that the signal obtained with this antibody in immunostaining experiments is in part due to labeling of paxillin. Published data need to be interpreted keeping in mind that images of phosphocaveolin-1 cellular localization obtained using this antibody are not valid. We re-evaluate the current knowledge about the role of caveolin-1 in cell adhesion and migration in view of this new information.
Hill
Michelle M
MM
Institute for Molecular Bioscience, University of Queensland, Brisbane, 4072, Australia.
Scherbakov
Nadja
N
Division of Cell Biology, Biocenter, Innsbruck Medical University, Fritz-Pregl-Str. 3, A-6020 Innsbruck, Austria.|Division of Physiology, Department of Physiology and Medical Physics, Innsbruck Medical University, Fritz-Pregl-Str. 3, A-6020 Innsbruck, Austria.
Schiefermeier
Natalia
N
Division of Cell Biology, Biocenter, Innsbruck Medical University, Fritz-Pregl-Str. 3, A-6020 Innsbruck, Austria.
Baran
JoAnne
J
Department of Anesthesiology Research, NE63 Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH, 44195, USA.
Hancock
John F
JF
Institute for Molecular Bioscience, University of Queensland, Brisbane, 4072, Australia.
Huber
Lukas A
LA
Division of Cell Biology, Biocenter, Innsbruck Medical University, Fritz-Pregl-Str. 3, A-6020 Innsbruck, Austria.
Parton
Robert G
RG
Institute for Molecular Bioscience, University of Queensland, Brisbane, 4072, Australia.|Centre for Microscopy and Microanalysis, University of Queensland, Brisbane, 4072, Australia.
Parat
Marie-Odile
MO
Department of Anesthesiology Research, NE63 Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH, 44195, USA.
D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D051242:Caveolin 1 / Q000378:metabolism / Q000502:physiology*; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement; D015151:Immunoblotting; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D051419:Paxillin / Q000378:metabolism; D010766:Phosphorylation; D013395:Sucrose / Q000494:pharmacology; D014443:Tyrosine / Q000737:chemistry
D051242:Caveolin 1; D051419:Paxillin; D014443:Tyrosine; D013395:Sucrose