Structural analysis of a water-soluble glucan (Fr.I) of an edible mushroom, Pleurotus sajor-caju.
A water-soluble glucan was obtained from the fruit bodies of an edible mushroom, Pleurotus sajor-caju, by hot water extraction, ethanol precipitation, dialysis, and Sepharose 6B gel filtration. On the basis of total hydrolysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, DQF-COSY, TOCSY, NOESY, and HSQC), the structure of the repeating unit of the glucan is determined as [carbohydrate structure: see text].
Pramanik
Malay
M
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721 102, West Bengal, India.
Chakraborty
Indranil
I
Mondal
Soumitra
S
Islam
Syed S
SS
D001671:Biochemistry / Q000379:methods*; D002845:Chromatography / Q000379:methods; D002849:Chromatography, Gas / Q000379:methods; D002850:Chromatography, Gel; D005936:Glucans / Q000737:chemistry*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry; D008745:Methylation; D010100:Oxygen / Q000737:chemistry; D010504:Periodic Acid / Q000737:chemistry; D020076:Pleurotus / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry; D012685:Sepharose / Q000737:chemistry; D014867:Water / Q000737:chemistry*
D005936:Glucans; D011134:Polysaccharides; D014867:Water; D010504:Periodic Acid; D012685:Sepharose; C009288:metaperiodate; D010100:Oxygen