title: Effect of low fluence diode laser irradiation on the hydraulic conductivity of perfused trabecular meshwork endothelial cell monolayers.
abstract: OBJECTIVE
  To determine the effect of low-fluence diode laser irradiation upon the fluid perfusion characteristics of cultured human trabecular meshwork cell monolayers when placed in a specially designed testing apparatus and subjected to fluid flow driven by a hydrostatic pressure gradient.
  
  
  METHODS
  Two experimental series were conducted. In the first series, six low-fluence diode laser irradiation experiments were conducted using cultured human trabecular meshwork cell monolayers grown on filter supports. Upon reaching a steady state perfusion condition at approximately 5.0 mmHg, monolayers were irradiated at fluencies ranging from 0.2619 to 0.8571 J/cm2 using a diode laser (lambda=810 nm). Perfusion and data collection continued for 45 minutes post-irradiation, after which the monolayers were tested to determine post-experimental viability. Hydraulic conductivity values were analyzed for post-irradiation response in 2.5-minute intervals, grouped by viability. In the second series, a total of six irradiated experiments and six simultaneous nonirradiated control experiments were conducted. Fluence values of 0.3571 J/cm2 (n=3) and 0.4286 J/cm2 (n=3) were used. Hydraulic conductivity values were analyzed for post-irradiation response in 2.5-minute intervals, grouped by irradiated vs. nonirradiated control groups.
  
  
  RESULTS
  In the first series, analysis showed that the viable monolayers exhibited a statistically significant increase in hydraulic conductivity (p<0.001) from 10 minutes post-irradiation onward. The non-viable monolayers exhibited a statistically significant decrease in hydraulic conductivity. In the second series, irradiated groups showed a significant difference (p<0.001) from nonirradiated controls from 10 minutes post-irradiation onward.
  
  
  CONCLUSIONS
  Low-fluence diode laser irradiation increases hydraulic conductivity in viable perfused TM cell monolayers when compared to baseline values or simultaneous nonirradiated controls while decreasing hydraulic conductivity in nonviable monolayers.
authors.affiliation: Department of Ophthalmology, The Ohio State University, Columbus 43210, USA. roberts.8@osu.edu
authors.forename: Cynthia J
authors.initials: CJ
authors.lastname: Roberts
authors.forename: Brian K
authors.initials: BK
authors.lastname: Rivera
authors.forename: Deborah M
authors.initials: DM
authors.lastname: Grzybowski
authors.forename: Ashraf M
authors.initials: AM
authors.lastname: Mahmoud
authors.forename: Paul A
authors.initials: PA
authors.lastname: Weber
mesh_terms: D001082:Aqueous Humor / Q000378:metabolism*; D001692:Biological Transport; D018938:Caco-2 Cells; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D005136:Eye Proteins / Q000378:metabolism; D005938:Glucocorticoids / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D006874:Hydrostatic Pressure; D028022:Low-Level Light Therapy; D008565:Membrane Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D014129:Trabecular Meshwork / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D062826:Zonula Occludens-1 Protein; D000099179:Myocilin
chemical_list: D003598:Cytoskeletal Proteins; D003907:Dexamethasone; D005136:Eye Proteins; D005938:Glucocorticoids; D006023:Glycoproteins; D008565:Membrane Proteins; D010750:Phosphoproteins; D062826:Zonula Occludens-1 Protein; D000099179:Myocilin; C569042:TJP1 protein, human