Roles for Drosophila melanogaster myosin IB in maintenance of enterocyte brush-border structure and resistance to the bacterial pathogen Pseudomonas entomophila.
Drosophila myosin IB (Myo1B) is one of two class I myosins in the Drosophila genome. In the larval and adult midgut enterocyte, Myo1B is present within the microvillus (MV) of the apical brush border (BB) where it forms lateral tethers between the MV membrane and underlying actin filament core. Expression of green fluorescent protein-Myo1B tail domain in the larval gut showed that the tail domain is sufficient for localization of Myo1B to the BB. A Myo1B deletion mutation exhibited normal larval gut physiology with respect to food uptake, clearance, and pH regulation. However, there is a threefold increase in terminal deoxynucleotidyl transferase dUTP nick-end labeling-positive enterocyte nuclei in the Myo1B mutant. Ultrastructural analysis of mutant midgut revealed many perturbations in the BB, including membrane tethering defects, MV vesiculation, and membrane shedding. The apical localization of both singed (fascin) and Dmoesin is impaired. BBs isolated from mutant and control midgut revealed that the loss of Myo1B causes the BB membrane and underlying cytoskeleton to become destabilized. Myo1B mutant larvae also exhibit enhanced sensitivity to oral infection by the bacterial pathogen Pseudomonas entomophila, and severe cytoskeletal defects are observed in the BB of proximal midgut epithelial cells soon after infection. Resistance to P. entomophila infection is restored in Myo1B mutant larvae expressing a Myo1B transgene. These results indicate that Myo1B may play a role in the local midgut response pathway of the Imd innate immune response to Gram-negative bacterial infection.
Hegan
Peter S
PS
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA. peter.hegan@yale.edu
Mermall
Valerie
V
Tilney
Lewis G
LG
Mooseker
Mark S
MS
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D004331:Drosophila melanogaster / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D020895:Enterocytes / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D041981:Gastrointestinal Tract; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D007814:Larva / Q000235:genetics / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D009154:Mutation / Q000235:genetics; D024461:Myosin Type I / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D011549:Pseudomonas / Q000472:pathogenicity*; D011552:Pseudomonas Infections / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D012680:Sensitivity and Specificity; D015996:Survival Rate
D024461:Myosin Type I