title: A two-dimensional proteome reference map of Herbaspirillum seropedicae proteins.
abstract: Herbaspirillum seropedicae is an endophytic diazotroph associated with economically important crops such as rice, sugarcane, and wheat. Here, we present a 2-D reference map for H. seropedicae. Using MALDI-TOF-MS we identified 205 spots representing 173 different proteins with a calculated average of 1.18 proteins/gene. Seventeen hypothetical or conserved hypothetical ORFs were shown to code for true gene products. These data will support the genome annotation process and provide a basis on which to undertake comparative proteomic studies.
authors.affiliation: Departamento de Bioquímica, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Daniela Fojo Seixas
authors.initials: DF
authors.lastname: Chaves
authors.forename: Pércio Pereira
authors.initials: PP
authors.lastname: Ferrer
authors.forename: Emanuel Maltempi
authors.initials: EM
authors.lastname: de Souza
authors.forename: Leonardo Magalhães
authors.initials: LM
authors.lastname: Gruz
authors.forename: Rose Adele
authors.initials: RA
authors.lastname: Monteiro
authors.forename: Fábio
authors.initials: F
authors.lastname: de Oliveira Pedrosa
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D042803:Herbaspirillum / Q000378:metabolism*; D020543:Proteome / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D020543:Proteome