[Analysis on the genetic structure of 8 Asia buffalo populations].
One hundred and forty seven alleles were detected when thirteen microsatellite loci were analyzed applying fluorescence-multiplex PCR technology in eight buffalo populations were analyzed, including six indigenous Chinese native breeds (DechangXinglongFuzhongWenzhouDongliuFu'an), and two introduced breeds (MurrahNili-Ravi). Seven populations have their own unique alleles, total number is twenty-three. As to all the eight populations, effective number of alleles (Ne) was between 2.2908 and 4.2308, heterozygosity (H) between 0.4951 and 0.7194, and polymorphism information content (PIC) between 0.4495 and 0.6776. Eleven of the thirteen microsatellite loci were of high polymorphism and were then the appropriate, polymorphism marker could be used to analyze properly genetic diversity of the involved buffalo populations. Cluster analysis indicated that Fuzhong and Dongliu were clustered together, then with an independent cluster of Xinglong. Wenzhou and Fu'an were clustered together, Dechang was an independent cluster. Murrah and Nili-Ravi were clustered together.
Wang
Dong-Lei
DL
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. donglei1110@126.com
Chang
Hong
H
Yang
Jun-Xiang
JX
Zhang
Gui-Xiang
GX
Wang
Zhi-Gang
ZG
Yu
Bo
B
Liao
Xin-Jun
XJ
Song
Wei-Tao
WT
Han
Xu
X
Song
Guang-Ming
GM
Wang
Jun-Hui
JH
Li
Rong-Ling
RL
D000818:Animals; D002020:Buffaloes / Q000145:classification / Q000235:genetics*; D016000:Cluster Analysis; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction