Antigen crosspresentation by human plasmacytoid dendritic cells.
Crosspresentation is a specialized function of myeloid dendritic cells (mDCs), allowing them to induce CD8+ T cell responses against exogenous antigens that are not directly produced in their cytotosol. Human plasmacytoid DCs (pDCs) are not considered so far as able to perform crosspresentation. We showed here that purified human pDCs crosspresented vaccinal lipopeptides and HIV-1 antigens from apoptotic cells to specific CD8+ T lymphocytes. Apoptotic debris were internalized by phagocytosis and the lipopeptide LPPol reached nonacidic endosomes. This crosspresentation was amplified upon influenza virus infection. Importantly, the efficiency of crosspresentation by pDCs was comparable to that of mDCs. This property of human pDCs needs to be taken into account to understand the pathogenesis of infectious, allergic, or autimmune diseases and to help achieve desired responses during vaccination by targeting specifically either type of DCs.
Hoeffel
Guillaume
G
Département d'Immunologie, Institut Cochin, Paris, F-75014, France.
Ripoche
Anne-Claire
AC
Matheoud
Diana
D
Nascimbeni
Michelina
M
Escriou
Nicolas
N
Lebon
Pierre
P
Heshmati
Farhad
F
Guillet
Jean-Gérard
JG
Gannagé
Monique
M
Caillat-Zucman
Sophie
S
Casartelli
Nicoletta
N
Schwartz
Olivier
O
De la Salle
Henri
H
Hanau
Daniel
D
Hosmalin
Anne
A
Marañón
Concepción
C
D016915:AIDS Vaccines / Q000276:immunology*; D017951:Antigen Presentation / Q000276:immunology*; D017209:Apoptosis / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D006678:HIV / Q000276:immunology; D015488:HIV Antigens / Q000276:immunology*; D006801:Humans; D007962:Leukocytes / Q000276:immunology / Q000821:virology; D008074:Lipoproteins / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D018613:Microscopy, Confocal; D014162:Transfection
D016915:AIDS Vaccines; D015488:HIV Antigens; D008074:Lipoproteins