Comparison of the antiviral potentials among the pseudorabies-resistant transgenes encoding different soluble forms of porcine nectin-1 in transgenic mice.
Nectin-1 is an alphaherpesvirus receptor that binds to virion glycoprotein D by the first immunoglobulin (Ig)-like domain. The possibility of making animals resistant to pseudorabies virus (PRV) infection has been investigated by generating transgenic mice expressing soluble forms of porcine nectin-1. Previously, transgenic mice were generated that expressed a fusion protein made of the entire ectodomain of nectin-1 fused to the Fc portion of human IgG, or the first Ig-like domain fused to the Fc portion of porcine IgG. Here, the contribution of the second and third Ig-like domains of nectin-1 was analysed by generating transgenic mice expressing the entire ectodomain of nectin-1 fused to the porcine Fc portion. Transgenic mice expressing each of three different fusion proteins were challenged with PRV for comparison of their resistance. Altogether, mice transgenic for a chimera that carried the entire ectodomain were more resistant than those transgenic for a chimera that carried the first Ig-like domain.
Ono
Etsuro
E
Laboratory of Animal Experiment for Disease Model, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan.|The Avian Zoonoses Research Center, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.|Laboratory of Biomedicine, Center of Biomedical Research, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Tomioka
Yukiko
Y
Laboratory of Animal Experiment for Disease Model, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan.
Watanabe
Yuki
Y
Sankyo Labo Service Corporation, Tokyo 132-0023, Japan.|Laboratory of Animal Experiment for Disease Model, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan.
Amagai
Keiko
K
Sankyo Labo Service Corporation, Tokyo 132-0023, Japan.|Laboratory of Animal Experiment for Disease Model, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan.
Morimatsu
Masami
M
Laboratory of Animal Experiment for Disease Model, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan.
Shinya
Kyoko
K
The Avian Zoonoses Research Center, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
Cherel
Pierre
P
France Hybrides, Saint-Jean-de-Braye, France.
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000276:immunology; D011558:Herpesvirus 1, Suid / Q000187:drug effects* / Q000235:genetics*; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D000075983:Nectins; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013552:Swine
D000998:Antiviral Agents; D015815:Cell Adhesion Molecules; D007141:Immunoglobulin Fc Fragments; C000619010:NECTIN1 protein, human; C000619015:Nectin1 protein, mouse; D000075983:Nectins; D011993:Recombinant Fusion Proteins