title: Higher rates of t(11;18) in Chinese patients with transformed type of MALT lymphoma suggest novel pathways for progression of the disease.
abstract: To detect the t(11;18) chromosome translocation in different stages of mucosa-associated lymphoid tissue (MALT) lymphoma, we established a RT-PCR method by adopting three new primer pairs and using the RNA extracted from the paraffin tissues to amplify the t(11;18) fusion gene API2-MALT1 in shorter lengths. Our results showed five key findings, which are (a) higher detection rates of t(11;18) (21.13%) in Chinese patients with transformed MALT lymphoma, (b) lower detection rates of t(11;18) in stomach MALT lymphoma, (c) different organ localizations of MALT lymphoma in Chinese patients, (d) higher nuclear expression rates of Bcl-10 in low grade MALT (51.72%), and (e) lower response rates (50% CR, and 50% PR) to anti-H.-pylori therapy. These findings suggest novel pathways for low-grade MALT lymphoma to be progressed into transformed MALT lymphoma. This study also suggests that amplification of shorter length of PCR products from the paraffin-fixed tissues increases sensitivity, which is significant in improving the selection of the therapeutic regimen and assessing the prognosis of the disease.
authors.affiliation: Department of Hematology and Lymphoma Research Center, Peking University Third Hospital, Peking University Health Science Center, Beijing, People's Republic of China. xiaoyank@yahoo.com
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ke
authors.forename: Linzhi
authors.initials: L
authors.lastname: Zhao
authors.forename: Zifen
authors.initials: Z
authors.lastname: Gao
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Dong
authors.forename: Xiaoge
authors.initials: X
authors.lastname: Zhou
authors.forename: Lin
authors.initials: L
authors.lastname: Shi
authors.forename: Xuebiao
authors.initials: X
authors.lastname: Huang
authors.forename: Hongmei
authors.initials: H
authors.lastname: Jing
authors.forename: Jijun
authors.initials: J
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Yang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D000465:Algorithms; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D000074018:B-Cell CLL-Lymphoma 10 Protein; D001483:Base Sequence; D016022:Case-Control Studies; D002681:China; D002880:Chromosomes, Human, Pair 11; D002887:Chromosomes, Human, Pair 18; D004252:DNA Mutational Analysis; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D005787:Gene Frequency; D016481:Helicobacter Infections / Q000150:complications / Q000188:drug therapy; D006801:Humans; D018442:Lymphoma, B-Cell, Marginal Zone / Q000150:complications / Q000235:genetics*; D008969:Molecular Sequence Data; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics*; D014178:Translocation, Genetic
chemical_list: C415943:API2-MALT1 fusion protein, human; D048868:Adaptor Proteins, Signal Transducing; D000900:Anti-Bacterial Agents; D000074018:B-Cell CLL-Lymphoma 10 Protein; C118591:BCL10 protein, human; D015514:Oncogene Proteins, Fusion