title: Diagnostic potential of recombinant protein of hexahistidine tag and infectious bursal disease virus VPX expressed in Escherichia coli.
abstract: The current method to detect antibody titre against infectious bursal disease virus (IBDV) in chickens is based on enzyme-linked immunosorbent assay (ELISA) using whole virus as coating antigen. Coating the ELISA plates requires a purified or at least semi-purified preparation of virus as antigen, which needs special skills and techniques. In this study, instead of using whole virus, recombinant protein of hexahistidine tag (His 6 tag) and VPX protein of IBDV expressed in E. coli was used as an alternative antigen to coat the ELISA plates. There was a good correlation coefficient (R2 = 0.972) between the results of the ELISA using plates coated with monoclonal antibody against His 6 tag and those of the commercial IBDV ELISA kit. Hence, His 6 tag and VPX recombinant protein expressed in E. coli has the potential for the development of ELISA for the measurement of IBDV-specific antibody.
authors.affiliation: Razi Research Institute, Arak, Iran.
authors.forename: S D
authors.initials: SD
authors.lastname: Hosseini
authors.forename: A R
authors.initials: AR
authors.lastname: Omar
authors.forename: I
authors.initials: I
authors.lastname: Aini
authors.forename: A M
authors.initials: AM
authors.lastname: Ali
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D018175:Birnaviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D015153:Blotting, Western / Q000662:veterinary; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D004926:Escherichia coli / Q000821:virology; D006639:Histidine / Q000235:genetics / Q000276:immunology*; D007243:Infectious bursal disease virus / Q000235:genetics / Q000276:immunology*; D009842:Oligopeptides / Q000235:genetics / Q000276:immunology*; D011201:Poultry Diseases / Q000175:diagnosis*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000956:Antigens, Viral; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D011994:Recombinant Proteins; D054334:Viral Regulatory and Accessory Proteins; D006639:Histidine