Molecular probe for enzymatic activity with dual output.
A novel molecular probe for enzymatic activity with a dual output detection-mode has been developed. The probe effectively detected the presence of the bacterial protease penicillin-G-amidase; a single cleavage by the enzyme initiated the fragmentation of a self immolative dendritic platform to release two reporter units. The signals of the free reporters were detected by two different spectroscopic techniques, fluorescence and UV-vis. This is the first reported molecular probe with two different chromogenic reporter units activated by a specific stimulus.
Danieli
Eyal
E
Department of Organic Chemistry, School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel-Aviv University, Tel Aviv 69978, Israel.
Shabat
Doron
D
D000634:Aminoquinolines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000187:drug effects*; D004789:Enzyme Activation / Q000187:drug effects; D008856:Microscopy, Fluorescence / Q000379:methods; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D009596:Nitrophenols / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010399:Penicillin Amidase / Q000187:drug effects*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013329:Structure-Activity Relationship
D000634:Aminoquinolines; D001426:Bacterial Proteins; D015335:Molecular Probes; D009596:Nitrophenols; C032928:6-aminoquinoline; D010399:Penicillin Amidase; C024836:4-nitrophenol