title: Acid proteinase, phospholipase, and biofilm production of Candida species isolated from blood cultures.
abstract: Three virulence factors comprising proteinase, phospholipase, and biofilm among 68 Candida albicans and 31 non-albicans Candida strains (11 C. tropicalis, 8 C. parapsilosis, 6 C. glabrata, 4 C. guillermondii, 2 C. krusei) isolated from blood cultures were analyzed. In total, 61 (89.7%) C. albicans strains were detected as proteinase positive whereas eight (25.8%) non-albicans Candida strains were proteinase positive (P < 0.05). Phospholipase production was detected in 41 (60.3%) C. albicans strains. All non-albicans Candida strains were phospholipase negative. Biofilm production was determined by both visual and spectrophotometric methods. Eight (11.8%) of C. albicans strains and 13 (41.93%) of 31 non-albicans Candida strains were biofilm positive with two of the methods (P < 0.05). According to our results, we may suggest that detection of hydrolytic enzyme and biofilm production abilities of the Candida isolates in clinical mycology laboratories may warn the clinician for a possible hematogenous infection.
authors.affiliation: Department of Clinical Microbiology, Marmara University School of Medicine, Haydarpasa, Istanbul 81326, Turkey.
authors.forename: Gulce
authors.initials: G
authors.lastname: Gokce
authors.forename: Nilgun
authors.initials: N
authors.lastname: Cerikcioglu
authors.forename: Aysegul
authors.initials: A
authors.lastname: Yagci
mesh_terms: D016282:Aspartic Acid Endopeptidases / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D002175:Candida / Q000201:enzymology / Q000502:physiology*; D002177:Candidiasis / Q000097:blood / Q000382:microbiology*; D003428:Cross Infection / Q000097:blood / Q000382:microbiology*; D006801:Humans; D010740:Phospholipases / Q000378:metabolism*
chemical_list: D010740:Phospholipases; D016282:Aspartic Acid Endopeptidases