Sigma factor genes sigC, sigE, and sigG are upregulated in heterocysts of the cyanobacterium Anabaena sp. strain PCC 7120.
We used gfp transcriptional fusions to investigate the regulation of eight sigma factor genes during heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120. Reporter strains containing gfp fusions with the upstream regions of sigB2, sigD, sigI, and sigJ did not show developmental regulation. Time-lapse microscopy of sigC, sigE, and sigG reporter strains showed increased green fluorescent protein fluorescence in differentiating cells at 4 h, 16 h, and 9 h, respectively, after nitrogen step down.
Aldea
M Ramona
MR
Department of Biology, Texas A&M University, 3258 TAMU, College Station, TX 77843-3258, USA.
Mella-Herrera
Rodrigo A
RA
Golden
James W
JW
D017033:Anabaena / Q000145:classification* / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D012808:Sigma Factor / Q000235:genetics*; D015854:Up-Regulation
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012808:Sigma Factor; C081467:sigC protein, Bacteria; C111259:sigE protein, Bacteria