title: Real-time spectral domain Doppler optical coherence tomography and investigation of human retinal vessel autoregulation.
abstract: Investigation of the autoregulatory mechanism of human retinal perfusion is conducted with a real-time spectral domain Doppler optical coherence tomography (SDOCT) system. Volumetric, time-sequential, and Doppler flow imaging are performed in the inferior arcade region on normal healthy subjects breathing normal room air and 100% oxygen. The real-time Doppler SDOCT system displays fully processed, high-resolution [512 (axial) x 1000 (lateral) pixels] B scans at 17 frames/sec in volumetric and time-sequential imaging modes, and also displays fully processed overlaid color Doppler flow images comprising 512 (axial) x 500 (lateral) pixels at 6 frames/sec. Data acquired following 5 min of 100% oxygen inhalation is compared with that acquired 5 min postinhalation for four healthy subjects. The average vessel constriction across the population is -16+/-26% after oxygen inhalation with a dilation of 36+/-54% after a return to room air. The flow decreases by -6+/-20% in response to oxygen and in turn increases by 21+/-28% as flow returns to normal in response to room air. These trends are in agreement with those previously reported using laser Doppler velocimetry to study retinal vessel autoregulation. Doppler flow repeatability data are presented to address the high standard deviations in the measurements.
authors.affiliation: Duke University, Department of Biomedical Engineering, Durham, North Carolina 27708, USA. bradley.bower@duke.edu
authors.forename: Bradley A
authors.initials: BA
authors.lastname: Bower
authors.forename: Mingtao
authors.initials: M
authors.lastname: Zhao
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Zawadzki
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Izatt
mesh_terms: D000465:Algorithms; D001783:Blood Flow Velocity / Q000502:physiology*; D003199:Computer Systems; D006487:Hemostasis / Q000502:physiology; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D010092:Oximetry / Q000379:methods*; D015203:Reproducibility of Results; D012171:Retinal Vessels / Q000033:anatomy & histology / Q000502:physiology*; D042262:Retinoscopy / Q000379:methods*; D012680:Sensitivity and Specificity; D041623:Tomography, Optical Coherence / Q000379:methods*