Virucidal efficacy of nine commercial disinfectants against porcine circovirus type 2.
A number of commercially available disinfectants are commonly used on pig breeding farms and are authorised by the French Agricultural Ministry. However, the efficacy of these disinfectants is unknown with regard to the emergent porcine circovirus type 2 (PCV2). The virucidal efficacy of nine disinfectants was evaluated by testing a suspension of PCV2 isolated in France. The assays were performed at 20 degrees C and the efficacy determined after 30 min contact time between virus and disinfectant. After this time, the mixture was passed through a detoxification column and then diluted to remove compounds toxic to the virus and the porcine kidney cell line. The filtrate was serially diluted and inoculated onto cell culture. The infectivity of PCV2 was determined by an immunoperoxidase monolayer assay. No reduction in PCV2 titre was demonstrated with iodine and phenolic products. Significant PCV2 titre reductions (1.61 log(10)) were noted for the seven other products. For five disinfectants, namely a product composed of potassium monopersulfate, two products comprising a quaternary ammonium with one or three aldehyde(s), sodium hypochlorite, and sodium hydroxide, the concentration that significantly reduced the PCV2 titre was equal or 1.5-4 times lower than the authorised use concentration. Only two disinfectants, one composed of potassium monopersulfate, the other containing peracetic acid with hydrogen peroxide, reduced the PCV2 titre with a product concentration at best equal or two times higher than the authorised use concentration.
Martin
Hélène
H
Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d'Etudes et de Recherches sur les Médicaments Vétérinaires et les Désinfectants, La Haute Marche, Javené, BP 90203 - 35302 Fougères cedex, France. h.martin@fougeres.afaas.fr
Le Potier
Marie-Frédérique
MF
Maris
Pierre
P
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D018173:Circoviridae Infections / Q000188:drug therapy / Q000517:prevention & control / Q000662:veterinary*; D017925:Circovirus / Q000187:drug effects*; D004202:Disinfectants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy* / Q000517:prevention & control; D013696:Temperature; D016896:Treatment Outcome
D000998:Antiviral Agents; D004202:Disinfectants