title: Virological and immunological characteristics of fatal Epstein-Barr virus mononucleosis in a 17-year-old Caucasian male presenting with meningoencephalitis and hemophagocytic syndrome.
abstract: In this report, the authors present a detailed immunological and virological assessment of an immunocompetent 17-year-old Caucasian male with a fatal Epstein-Barr virus (EBV) infectious mononucleosis presenting with meningoencephalitis and hemophagocytic syndrome. The patient with serologically confirmed EBV infectious mononucleosis was admitted to the hospital because of 3 weeks' fever. Fine-needle aspiration of lymph nodes showed reactive hyperplasia with prominent hemophagocytosis. Percentages of intracellular interferon-gamma (IFN-gamma) in CD4(+) and CD8(+) T cells in the peripheral blood progressively increased during the course of disease (10.2% and 8.5% on day 35; 30.1% and 53.2% on day 44; 42.2% and 75.2% on day 50; 36.1% and 50.6% on day 59, respectively). On day 50, the patient developed meningoencephalitis. Brain computed tomography (CT) was normal. Brain magnetic resonance imaging (MRI) showed multifocal inflammatory lesions in frontal and temporal cortex of the right hemisphere as well as severe perivascular inflammatory reaction. The patient was treated with steroids, cyclosporin A, and methotrexate intratecally. Following treatment, EBV viremia in the blood and cerebrospinal fluid (CSF) decreased from pretreatment values (54,490 copies of EBV DNA/ml and 39,500 copies/ml, respectively) to 8715 copies/ml in the blood and 14,690 in the CSF. Despite treatment, the patient remained unconscious and died of sepsis and pneumonia 3 months after initial symptoms. Immunohistochemical staining showed the presence of EBV in both perivascular infiltrates and grey matter. Enhanced Th1 response as shown by high levels of IFN-gamma in peripheral blood lymphocytes may be a predictor of severe complications during acute EBV infection. Early implementation of immunosuppressive therapy in these patients should be considered.
authors.affiliation: Department for Viral Hepatitis and Laboratory for Molecular Diagnostics, University Hospital for Infectious Diseases "Dr. Fran Mihaljevic,", Zagreb, Croatia.
authors.forename: Adriana
authors.initials: A
authors.lastname: Vince
authors.forename: Snjezana Zidovec
authors.initials: SZ
authors.lastname: Lepej
authors.forename: Ivan
authors.initials: I
authors.lastname: Kurelac
authors.forename: Bruno
authors.initials: B
authors.lastname: Barsic
authors.forename: Sanja
authors.initials: S
authors.lastname: Kozic
authors.forename: Igor
authors.initials: I
authors.lastname: Klinar
authors.forename: Kamelia
authors.initials: K
authors.lastname: Zarkovic
mesh_terms: D000293:Adolescent; D001706:Biopsy; D001921:Brain / Q000473:pathology / Q000821:virology; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007244:Infectious Mononucleosis / Q000276:immunology* / Q000473:pathology; D007371:Interferon-gamma / Q000378:metabolism; D016131:Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D051359:Lymphohistiocytosis, Hemophagocytic / Q000276:immunology* / Q000473:pathology / Q000821:virology*; D008297:Male; D008590:Meningoencephalitis / Q000276:immunology* / Q000473:pathology / Q000821:virology*
chemical_list: D007371:Interferon-gamma