Separation of proteins including metallothionein in cerebrospinal fluid by size exclusion HPLC and determination of trace elements by HR-ICP-MS.
A method to study the protein binding patterns of trace elements in human cerebrospinal fluid (CSF) is described. Proteins in CSF samples were separated by size exclusion chromatography combined with high performance liquid chromatography (SEC-HPLC). The column was calibrated to separate proteins in the molecular weight range 6-70 kDa. Fractions were then analyzed off-line for trace elements using high resolution inductively coupled plasma mass spectrometry (HR-ICP-MS). We were able to accurately determine more than 10 elements of clinical interest in the CSF fractions. Results are presented for Cd, Mn, Fe, Pb, Cu and Zn. The total concentrations of 16 trace elements in human plasma and CSF are also presented. The method was able to differentiate the relative contribution of metallothionein and other proteins towards metal binding in human CSF.
Gellein
Kristin
K
Department of Chemistry, Norwegian University of Science and Technology, NO-7491 Trondheim, Norway.
Roos
Per M
PM
Evje
Lars
L
Vesterberg
Olof
O
Flaten
Trond Peder
TP
Nordberg
Monica
M
Syversen
Tore
T
D000328:Adult; D002104:Cadmium / Q000134:cerebrospinal fluid; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards*; D003300:Copper / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D007501:Iron / Q000134:cerebrospinal fluid; D007854:Lead / Q000134:cerebrospinal fluid; D008297:Male; D008345:Manganese / Q000134:cerebrospinal fluid; D008668:Metallothionein / Q000134:cerebrospinal fluid* / Q000737:chemistry; D008875:Middle Aged; D008970:Molecular Weight; D010316:Particle Size; D011786:Quality Control; D015203:Reproducibility of Results; D014131:Trace Elements / Q000134:cerebrospinal fluid*; D015032:Zinc / Q000134:cerebrospinal fluid
D014131:Trace Elements; D002104:Cadmium; D007854:Lead; D008345:Manganese; D003300:Copper; D008668:Metallothionein; D007501:Iron; D015032:Zinc