In situ generation of oxo-sulfidobis(dithiolene)tungsten(VI) complexes: active-site models for the aldehyde ferredoxin oxidoreductase family of tungsten enzymes.
Oxo-sulfidobis(dithiolene)tungsten(VI) complexes were prepared in situ by the reaction of oxobis(dithiolene)tungsten(V) precursors with hydrosulfide (SH-). The complexes, characterized by UV-vis, electrospray ionization mass spectrometry, IR, and resonance Raman spectroscopies, model the proposed coordination environment and observed hydrolytic reactions of members of the aldehyde ferredoxin oxidoreductase family of tungsten enzymes.
Sugimoto
Hideki
H
Department of Chemistry, Graduate School of Science, Osaka City University, Osaka 558-8585, Japan. sugimoto@sci.osaka-cu.ac.jp
Tano
Hiroyuki
H
Tajima
Reiko
R
Miyake
Hiroyuki
H
Tsukube
Hiroshi
H
Ohi
Hiromi
H
Itoh
Shinobu
S
D001665:Binding Sites; D015392:Chemistry, Inorganic / Q000379:methods*; D007700:Kinetics; D008024:Ligands; D008956:Models, Chemical; D008968:Molecular Conformation; D015394:Molecular Structure; D050743:Pyruvate Synthase / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013055:Spectrophotometry, Infrared / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods; D013379:Substrate Specificity; D014414:Tungsten / Q000032:analysis*
D008024:Ligands; D050743:Pyruvate Synthase; D014414:Tungsten