title: A PCR-based method to differentiate between Acinetobacter baumannii and Acinetobacter genomic species 13TU.
abstract: A new PCR-based method that exploits differences in gyrB gene sequences was developed to distinguish between Acinetobacter baumannii and Acinetobacter genomic sp. 13TU. Among 118 clinical and reference Acinetobacter strains, 102 of which were previously speciated by amplified rDNA restriction analysis as belonging to the Acinetobacter calcoaceticus-A. baumannii complex, the method correctly identified 31 A. baumannii and 54 Acinetobacter genomic sp. 13TU isolates to the species level. The method was rapid, specific and easy to interpret.
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany.
authors.forename: P G
authors.initials: PG
authors.lastname: Higgins
authors.forename: H
authors.initials: H
authors.lastname: Wisplinghoff
authors.forename: O
authors.initials: O
authors.lastname: Krut
authors.forename: H
authors.initials: H
authors.lastname: Seifert
mesh_terms: D000150:Acinetobacter / Q000145:classification* / Q000302:isolation & purification*; D000151:Acinetobacter Infections / Q000382:microbiology*; D027081:DNA Gyrase / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D027081:DNA Gyrase