title: [Murine ciliary body pigment epithelial cells inhibit activation of T cells].
abstract: OBJECTIVE
  Pigment epithelial(PE) cells cultured from the eye possess the novel property of suppressing T cells in vitro. Although cultured iris PE (IPE) and retinal PE (RPE) achieve suppression, the function of ciliary body PE (CBPE) is still unknown. We investigated whether CBPE can suppress the activation of T cells in vitro.
  
  
  METHODS
  CBPE, IPE and RPE cells(controls) were established from normal C57BL/6 mice. T-cell activation was assessed by proliferative response and cytokine production, by ELISA. The expression of candidate genes on ocular PE was evaluated with on oligonucleotide microarray, and PE proliferation and PE morphology were also evaluated.
  
  
  RESULTS
  CBPE significantly suppressed T cell activation in vitro. Cell division of CBPE was much greater than in other PE, and the cells were confluent from on early phase. Microarray analysis showed that the expression of candidate genes in CBPE is similar to the expression in other PE cells.
  
  
  CONCLUSIONS
  CBPE fully suppress T cell activation. Thus, ocular pigment epithelial cells display inhibitory function in the immune privileged site.
authors.affiliation: Department of Ophthalmology & Visual Science, Tokyo Medical and Dental University Graduate School of Medicine, Tokyo, Japan. sunaph@tmd.ac.jp
authors.forename: Sunao
authors.initials: S
authors.lastname: Sugita
authors.forename: Yuri
authors.initials: Y
authors.lastname: Futagami
authors.forename: Shintaro
authors.initials: S
authors.lastname: Horie
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Aburatani
authors.forename: Manabu
authors.initials: M
authors.lastname: Mochizuki
mesh_terms: D000818:Animals; D002924:Ciliary Body / Q000276:immunology*; D008213:Lymphocyte Activation / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010857:Pigment Epithelium of Eye / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*