The genotoxicity study of garlic and pasipy herbal drops by peripheral blood micronucleus test.
The in vivo rodent micronucleus test is widely used as a genotoxic assay to detect the clastogenic activity of chemicals. In this research the genotoxic effects of herbal drops of garlic and pasipy were evaluated using the micronucleus test. Maximum Tolerated Dose (MTD) was determined by a dose-response test. For each medicine three treatment groups were considered with doses of MTD, 1/2 MTD and 1/4 MTD according to the CSGMT protocol (1995 Japan). Drugs were administered orally to mice (test groups). Mitomicin C was used as a known genotoxic agent in positive control group. The peripheral blood samples before treatment (zero time samples) were considered as negative control. The appearance of a micronucleus is used as an index for genotoxic potential. The results obtained indicated that the herbal drops showed genotoxicity effect and it was dose-dependent compared to the negative control group. This genotoxicity was significant (p < 0.05) but the genotoxic effects of garlic and pasipy were "not significant" compared to the historical negative control group (p > 0.05). Therefore our results if compared to the negative control group is significant and it is worthy of consideration.
Kalantari
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School of Pharmacy, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran. Hkalantari@ajums.ac.ir
Larki
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Latifi
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D000284:Administration, Oral; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005737:Garlic; D008297:Male; D020714:Maximum Tolerated Dose; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests; D016685:Mitomycin / Q000633:toxicity; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*; D031683:Passifloraceae; D028321:Plant Preparations / Q000633:toxicity; D012156:Reticulocytes / Q000187:drug effects*
D009153:Mutagens; D028321:Plant Preparations; D016685:Mitomycin