title: High-resolution water window X-ray imaging of in vivo cells and their products using LiF crystal detectors.
abstract: High contrast imaging of in vivo Chlorella sorokiniana cells with submicron spatial resolution was obtained with a contact water window X-ray microscopy technique using a point-like, laser-plasma produced, water-window X-ray radiation source, and LiF crystals as detectors. This novel type of X-ray imaging detectors is based on photoluminescence of stable electronic point defects, characterized by high intrinsic resolution. The fluorescence images obtained on LiF crystals exposed in single-shot experiments demonstrate the high sensitivity and dynamic range of this new detector. The powerful performances of LiF crystals allowed us to detect the exudates of Chlorella cells in their living medium and their spatial distribution in situ, without any special sample preparation.
authors.affiliation: Physics Technologies and New Materials Department, ENEA, CR Frascati, 00044 Frascati, Rome, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Bonfigli
authors.forename: Anatoly
authors.initials: A
authors.lastname: Faenov
authors.forename: Francesco
authors.initials: F
authors.lastname: Flora
authors.forename: Massimo
authors.initials: M
authors.lastname: Francucci
authors.forename: Pasqualino
authors.initials: P
authors.lastname: Gaudio
authors.forename: Antonia
authors.initials: A
authors.lastname: Lai
authors.forename: Sergio
authors.initials: S
authors.lastname: Martellucci
authors.forename: Rosa Maria
authors.initials: RM
authors.lastname: Montereali
authors.forename: Tania
authors.initials: T
authors.lastname: Pikuz
authors.forename: Lucia
authors.initials: L
authors.lastname: Reale
authors.forename: Maria
authors.initials: M
authors.lastname: Richetta
authors.forename: Maria Aurora
authors.initials: MA
authors.lastname: Vincenti
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Baldacchini
mesh_terms: D002708:Chlorella / Q000166:cytology* / Q000378:metabolism; D003460:Crystallization; D005459:Fluorides / Q000737:chemistry*; D018020:Lithium Compounds / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D014965:X-Rays
chemical_list: D018020:Lithium Compounds; C027651:lithium fluoride; D005459:Fluorides