Statistical analysis of amplified fragment length polymorphism data: a toolbox for molecular ecologists and evolutionists.
Recently, the amplified fragment length polymorphism (AFLP) technique has gained a lot of popularity, and is now frequently applied to a wide variety of organisms. Technical specificities of the AFLP procedure have been well documented over the years, but there is on the contrary little or scattered information about the statistical analysis of AFLPs. In this review, we describe the various methods available to handle AFLP data, focusing on four research topics at the population or individual level of analysis: (i) assessment of genetic diversity; (ii) identification of population structure; (iii) identification of hybrid individuals; and (iv) detection of markers associated with phenotypes. Two kinds of analysis methods can be distinguished, depending on whether they are based on the direct study of band presences or absences in AFLP profiles ('band-based' methods), or on allelic frequencies estimated at each locus from these profiles ('allele frequency-based' methods). We investigate the characteristics and limitations of these statistical tools; finally, we appeal for a wider adoption of methodologies borrowed from other research fields, like for example those especially designed to deal with binary data.
Bonin
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A
Diversity Arrays Technology P/L, Yarralumla, ACT 2600, Australia.
Ehrich
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D
Manel
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D005075:Biological Evolution; D019295:Computational Biology / Q000379:methods; D003198:Computer Simulation; D004463:Ecology / Q000379:methods; D005787:Gene Frequency; D005819:Genetic Markers; D005821:Genetic Techniques; D006824:Hybridization, Genetic; D010641:Phenotype; D011110:Polymorphism, Genetic; D012984:Software
D005819:Genetic Markers