Characterisation of the warm acclimated protein gene (wap65) in the Antarctic plunderfish (Harpagifer antarcticus).
Physiological adaptation to increased environmental temperatures has been studied experimentally in a number of fish species, with the up-regulation of several genes identified as being associated with the process, such as the warm-acclimated protein (wap65). This article describes the cloning and characterisation of the wap65-2 gene from the Antarctic plunderfish (Harpagifer antarcticus). The transcriptional expression of this gene in response to elevated seawater temperatures over a time course series is presented. Initially there is strong down-regulation of this gene to a maximum of 40-fold within 4 h, followed by recovery to almost control levels within 48 h, indicating that this gene does not play a role in the potential temperature adaptation of H. antarcticus.
Clark
Melody S
MS
British Antarctic Survey, Natural Environment Research Council, High Cross, Madingley Road, Cambridge CB3 0ET, UK. mscl@bas.ac.uk
Burns
Gavin
G
D000064:Acclimatization / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D000864:Antarctic Regions; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics*
D029941:Fish Proteins