A transgenic mouse class-III beta tubulin reporter using yellow fluorescent protein.
A yellow fluorescence protein (YFP) reporter construct was cloned downstream of the beta-tubulin III promoter and injected to produce two founder lines of transgenic mice. YFP expression was observed in many regions of the developing peripheral and central nervous system. YFP expression was first observed in the peripheral and central nervous system as early as embryonic day 9.0. There was a dramatic increase in the number of neuronal systems expressing YFP through P0. Then as the animals reached adult age, the expression levels decreased, but many neurons still show YFP expression, notably in regions of the brain undergoing adult neurogenesis, i.e., the rostral migratory stream and subgranular layer of the dentate gyrus. This reporter-based staining was compared with anti-class-III beta-tubulin immunocytochemistry and shown to closely parallel the expression of the endogenous protein. These transgenic lines should provide unique models to study in vivo and in vitro neurodevelopment.
Liu
Li
L
Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA. lliu8@utmem.edu
Geisert
Eldon E
EE
Frankfurter
Anthony
A
Spano
Anthony J
AJ
Jiang
Chloe Xue
CX
Yue
Junming
J
Dragatsis
Ioannis
I
Goldowitz
Dan
D
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D003001:Cloning, Molecular; D047108:Embryonic Development / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D014018:Tissue Distribution; D014404:Tubulin / Q000235:genetics* / Q000378:metabolism
D008164:Luminescent Proteins; D014404:Tubulin