[Drought resistance and heredity of transgenic tobacco with OsMAPK4 gene].
OsMAPK4 gene was obtained by RT-PCR using gene special primer from Liaoyan241 leaf treated with low temperature. Plant expression vector pBME12 was constructed in which OsMAPK4 gene was regulated by E12 promoter. OsMAPK4 gene was transferred into tobacco via Agrobacterium-mediated method and 25 transgenic plants obtained. The results of drought resistance experiments showed that overexpression of OsMAPK4 gene conferred enhanced drought resistance of T1 transgenic plants. Kanamycin-resistance segregation in T1 transgenic plants showed that the most transgenic lines were in single gene inheritance.
Li
Jie
J
Life Science College, Northeast Agricultural University, Harbin 150030, China E-mail. lijie_neau@126.com
Qi
Yan
Y
Li
Ying
Y
Liu
Xi-Yan
XY
Ji
Haobutao
H
Zhu
Yan-Ming
YM
Bai
Xi
X
Cai
Hua
H
D000222:Adaptation, Physiological / Q000235:genetics / Q000502:physiology; D055864:Droughts; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014026:Nicotiana / Q000235:genetics* / Q000502:physiology
D010940:Plant Proteins; D020928:Mitogen-Activated Protein Kinases