A poly(cyclopentadithiophene) matrix suitable for electrochemically controlled DNA delivery.
A conducting polymer is tested for DNA delivery trials. The conducting matrix used is successful for electrochemical delivery of DNA accumulated by covalent immobilization. The electrochemical process consists of the reduction of arylsulfonamide moieties, which occur as linker groups. The specific design of the polymer allows the electronic properties to be promoted, making available the cleavage potential in physiological media. The amount of DNA released from a modified platinum electrode is investigated by quartz crystal microbalance. The released species used to prove the system performance are long sequences of DNA strands, which are amplified by PCR after liberation and identified by electrophoresis migration.
Gautier
Christelle
C
Cougnon
Charles
C
Pilard
Jean-François
JF
Casse
Nathalie
N
Chénais
Benoît
B
D003517:Cyclopentanes / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry; D013876:Thiophenes / Q000737:chemistry*
D003517:Cyclopentanes; D009841:Oligonucleotides; D013876:Thiophenes; C524746:poly(cyclopentadithiophene); D004247:DNA