title: Chaperone-dependent E3 ligase CHIP ubiquitinates and mediates proteasomal degradation of soluble guanylyl cyclase.
abstract: The nitric oxide receptor soluble guanylyl cyclase (sGC) exists in multimeric protein complexes, including heat shock protein (HSP) 90 and endothelial nitric oxide synthase. Inhibition of HSP90 by geldanamycin causes proteasomal degradation of sGC protein. In this study, we have investigated whether COOH terminus of heat shock protein 70-interacting protein (CHIP), a co-chaperone molecule that is involved in protein folding but is also a chaperone-dependent ubiquitin E3 ligase, could play a role in the process of degradation of sGC. Transient overexpression of CHIP in COS-7 cells degraded heterologous sGC in a concentration-related manner; this downregulation of sGC was abrogated by the proteasome inhibitor MG-132. Transfection of tetratricopeptide repeats and U-box domain CHIP mutants attenuated sGC degradation, suggesting that both domains are indispensable for CHIP function. Results from immunoprecipitation and indirect immunofluorescent microscopy experiments demonstrated that CHIP is associated with sGC, HSP90, and HSP70 in COS-7 cells. Furthermore, CHIP increased the association of HSP70 with sGC. In in vitro ubiquitination assays using purified proteins and ubiquitin enzymes, E3 ligase CHIP directly ubiquitinated sGC; this ubiquitination was potentiated by geldanamycin in COS-7 cells, followed by proteasomal degradation. In rat aortic smooth muscle cells, endogenous sGC was also degraded by adenovirus-infected wild-type CHIP but not by the chaperone interaction-deficient K30A CHIP, whereas CHIP, but not K30A, attenuated sGC expression in, and nitric oxide donor-induced relaxation of, rat aortic rings, suggesting that CHIP plays a regulatory role under physiological conditions. This study reveals a new mechanism for the regulation of sGC, an important mediator of cellular and vascular function.
authors.affiliation: Vascular Biology Center, Medical College of Georgia, Augusta, GA 30912-2500, USA.
authors.forename: Tian
authors.initials: T
authors.lastname: Xia
authors.forename: Christiana
authors.initials: C
authors.lastname: Dimitropoulou
authors.forename: Jingmin
authors.initials: J
authors.lastname: Zeng
authors.forename: Galina N
authors.initials: GN
authors.lastname: Antonova
authors.forename: Connie
authors.initials: C
authors.lastname: Snead
authors.forename: Richard C
authors.initials: RC
authors.lastname: Venema
authors.forename: David
authors.initials: D
authors.lastname: Fulton
authors.forename: Shuibing
authors.initials: S
authors.lastname: Qian
authors.forename: Cam
authors.initials: C
authors.lastname: Patterson
authors.forename: Andreas
authors.initials: A
authors.lastname: Papapetropoulos
authors.forename: John D
authors.initials: JD
authors.lastname: Catravas
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D006162:Guanylate Cyclase / Q000378:metabolism*; D050883:HSC70 Heat-Shock Proteins / Q000378:metabolism*; D018832:Molecular Chaperones / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D012995:Solubility; D000071756:Soluble Guanylyl Cyclase; D025801:Ubiquitin / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
chemical_list: D050883:HSC70 Heat-Shock Proteins; D018832:Molecular Chaperones; D018160:Receptors, Cytoplasmic and Nuclear; D025801:Ubiquitin; D044767:Ubiquitin-Protein Ligases; D046988:Proteasome Endopeptidase Complex; D006162:Guanylate Cyclase; D000071756:Soluble Guanylyl Cyclase