Laboratory production of 100 base pair DNA molecular weight markers.
DNA molecular weight markers are routinely used in agarose gel electrophoresis. Here we report a method called PCR-synthesized marker (PSM) to generate DNA molecular rulers by PCR in the laboratory. This strategy can also be used to produce 100 bp RNA molecular weight markers by run-off transcription.
Chang
Microsugar
M
Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan.
Wang
Jhih-Hao
JH
Lee
Han-Jung
HJ
D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D008970:Molecular Weight
D004247:DNA