Proteome of murine jejunal brush border membrane vesicles.
The first detailed description of the proteome of the mouse jejunal brush border membrane vesicle is presented here. This was obtained by a combination of purification via divalent (Mg2+) cation precipitation starting with isolated cells plus strong cation exchange chromatography LC-MS/MS. Five-hundred seventy proteins were identified including 45 transport proteins. Among the latter, 18 had either not been identified in the intestine in the past or there was a single unconfirmed report of their presence. Validation was accomplished by a combination of immunoblotting and immunofluorescence using mouse jejunum and previously described antibodies. The validated BB proteins were aquaporin 7, Glut 9b, Na+I- symporter (NIS), and non-gastric H+/K+-ATPase. This study helps to further define the brush border membrane vesicle, a preparation which has been widely used to identify transport function of the small intestine.
Donowitz
Mark
M
Singh
Siddharth
S
Salahuddin
Farah F
FF
Hogema
Boris M
BM
Chen
Yueping
Y
Gucek
Marjan
M
Cole
Robert N
RN
Ham
Amy
A
Zachos
Nicholas C
NC
Kovbasnjuk
Olga
O
Lapierre
Lynne A
LA
Broere
Nellie
N
Goldenring
James
J
deJonge
Hugo
H
Li
Xuhang
X
D000818:Animals; D002853:Chromatography, Liquid; D007413:Intestinal Mucosa / Q000378:metabolism; D007583:Jejunum / Q000378:metabolism*; D008297:Male; D051379:Mice; D008871:Microvilli / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
D020543:Proteome