Two members of the Arabidopsis CLC (chloride channel) family, AtCLCe and AtCLCf, are associated with thylakoid and Golgi membranes, respectively.
Though numerous pieces of evidence point to major physiological roles for anion channels in plants, progress in the understanding of their biological functions is limited by the small number of genes identified so far. Seven chloride channel (CLC) members could be identified in the Arabidopsis genome, amongst which AtCLCe and AtCLCf are both more closely related to bacterial CLCs than the other plant CLCs. It is shown here that AtCLCe is targeted to the thylakoid membranes in chloroplasts and, in agreement with this subcellular localization, that the clce mutants display a phenotype related to photosynthesis activity. The AtCLCf protein is localized in Golgi membranes and functionally complements the yeast gef1 mutant disrupted in the single CLC gene encoding a Golgi-associated protein.
Marmagne
Anne
A
Institut des Sciences du Végétal, Centre National de la Recherche Scientifique (CNRS-UPR 2355), Bât 22, avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France.
Vinauger-Douard
Marion
M
Monachello
Dario
D
de Longevialle
Andéol Falcon
AF
Charon
Céline
C
Allot
Michèle
M
Rappaport
Fabrice
F
Wollman
Francis-André
FA
Barbier-Brygoo
Hélène
H
Ephritikhine
Geneviève
G
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D015153:Blotting, Western; D018118:Chloride Channels / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D018745:Genome, Plant; D006056:Golgi Apparatus / Q000378:metabolism*; D010788:Photosynthesis; D013050:Spectrometry, Fluorescence; D020524:Thylakoids / Q000378:metabolism*
D018118:Chloride Channels