Protective effects of erdosteine and vitamins C and E combination on ischemia-reperfusion-induced lung oxidative stress and plasma copper and zinc levels in a rat hind limb model.
The aim of this study was to investigate the protective effects of erdosteine and vitamins C and E (VCE) on the lungs after performing hind limb ischemia-reperfusion (I/R) by assessing oxidative stress, plasma copper (Cu), and zinc (Zn) analysis. The animals were divided randomly into four groups as nine rats each as follows: control, I/R, I/R plus erdosteine, and I/R plus VCE combination. I/R period for 60 min was performed on the both hind limbs of all the rats in the groups of I/R, erdosteine with I/R, VCE with I/R allowing 120 min of reperfusion. The animals received orally erdosteine one time in a day and 3 days before I/R in the erdosteine group. In the VCE group, the animals VCE combination received one time in a day and 3 days before I/R, although placebo was given to control and I/R group animals. Lung lipid peroxidation (malondialdehyde [MDA]) level, superoxide dismutase (SOD), and catalase activities were increased, although lung glutathione (GSH) and plasma Zn levels decreased in I/R group in lung tissue compared with the control group. Serum MDA level, creatine kinase, and lactate dehydrogenase activities were increased in I/R group compared with the control. Lung MDA and plasma Zn levels and lung SOD activity were decreased by erdosteine administration, whereas lung GSH levels after I/R increased. The plasma Zn levels and lung SOD activity were decreased by VCE administration, although the plasma Cu and lung GSH levels increased after I/R. In conclusion, erdosteine has an antioxidant role on the values in the rat model, and it has more protective affect than in VCE in attenuating I/R-induced lung injury in rats.
Sirmali
Mehmet
M
Department of Thoracic Surgery, Süleyman Demirel University Medical School, Isparta, Turkey. mehmetsirmali@yahoo.com
Uz
Efkan
E
Sirmali
Rana
R
Kilbaş
Aynur
A
Yilmaz
H Ramazan
HR
Altuntaş
Irfan
I
Naziroğlu
Mustafa
M
Delibaş
Namik
N
Vural
Hüseyin
H
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism*; D003300:Copper / Q000097:blood*; D003402:Creatine Kinase / Q000378:metabolism; D005100:Expectorants / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D035002:Lower Extremity; D008168:Lung / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D020011:Protective Agents / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood*; D013864:Thioglycolates / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism*; D015032:Zinc / Q000097:blood*
D000975:Antioxidants; D005100:Expectorants; D020011:Protective Agents; D013864:Thioglycolates; D013876:Thiophenes; D014810:Vitamin E; D008315:Malondialdehyde; C048498:erdosteine; D003300:Copper; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D005978:Glutathione; D015032:Zinc; D001205:Ascorbic Acid