title: Initial analysis of tandemly repetitive sequences in the genome of Zhikong scallop (Chlamys farreri Jones et Preston).
abstract: Tandemly repetitive sequences are widespread in all eukaryotic genomes, but data on tandem repeats are limited in Zhikong scallop (Chlamys farreri). In the present study, paired-end sequencing of 2016 individual fosmid clones resulted in 3646 sequences. A total of 2,286,986 bp of genomic sequences were generated, representing approximately 1.84 per thousand of the Zhikong scallop genome. Using tandem repeats finder (TRF) software, a total of 2500 tandem repeats were found, including 313 satellites, 1816 minisatellites and 371 microsatellites. The cumulative length of tandem repeats was 552,558 bp, accounting for 24.16% of total length. Specifically, the length of microsatellites, minisatellites and satellites was 9425, 336,001 and 207,132 bp, accounting for 1.71, 60.81 and 37.49% of the length of tandem repeats, and 0.41, 14.69 and 9.06% of total length, respectively. The detailed information on the characteristic of all repeat units was also represented, which will provide a useful resource for physical mapping and better utilization of the existing genomic information in Zhikong scallop.
authors.affiliation: Division of Life Science and Technology, Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao, People's Republic of China. xiao_ling_ling@hotmail.com
authors.forename: Lingling
authors.initials: L
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Cheng
authors.forename: Shi
authors.initials: S
authors.lastname: Wang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Hu
authors.forename: Jingjie
authors.initials: J
authors.lastname: Hu
authors.forename: Zhenmin
authors.initials: Z
authors.lastname: Bao
mesh_terms: D000818:Animals; D001483:Base Sequence; D016678:Genome / Q000235:genetics*; D008969:Molecular Sequence Data; D049897:Pectinidae / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000662:veterinary; D020080:Tandem Repeat Sequences / Q000235:genetics*