Flavonoid rutin alters the viability and function of mitogen-stimulated splenocytes and thymocytes compared with non stimulated cells.
Rutin is a flavonoid obtained from Dimorphandra mollis (Benth.), a medicinal Brazilian plant used as antioxidative, antihemorrhagic, and blood vessel protector. The present study has examined its effects on the viability and function of immune system cells in vitro. Rat spleen and thymus cells were cultured with 10 nM, 1 microM, and 10 microM of the drug in the presence or absence of PWM, LPS, or ConA mitogens. Cellular proliferation was analyzed by H(3)-thymidin uptake and IFN-gamma and IL-10 were measured by ELISA after 48 and 72 hr. Viability was measured by flow cytometry using Annexin V and PI after 24 and 48 hr. The flavonoid rutin inhibited splenocytes and thymocytes proliferation under ConA stimulation observed by an increase on apoptosis levels of thymocytes stimulated with PWM in 24 hr and on splenocytes stimulated with PWM in 48 hr. Function studies showed a decrease on IFN-gamma production by splenocytes and thymocytes stimulated with PWM or ConA. Spleen cells cultured with LPS and rutin showed a decrease on apoptosis after 24 hr and an increase on the IL-10 levels after 48 hr. There was no significant variation on the necrosis rate, viability, and function of cells treated with rutin in the absence of mitogenic stimulus.
Roseghini
R
R
Programa de Pós-Graduação em Imunologia, Universidade Federal da Bahia, Bahia, Brazil. bino7@ig.com.br
Rocha
D S
DS
Clarêncio
J
J
Costa
S L
SL
Costa
M F D
MF
Tardy
M
M
Nascimento
R
R
Schaer
R
R
Velozo
E
E
Meyer
R
R
Freire
S
S
D000818:Animals; D017304:Annexin A5 / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D003208:Concanavalin A / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008934:Mitogens / Q000494:pharmacology*; D009336:Necrosis; D011043:Pokeweed Mitogens / Q000494:pharmacology; D051381:Rats; D012431:Rutin / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism
D017304:Annexin A5; D016207:Cytokines; D008070:Lipopolysaccharides; D008934:Mitogens; D011043:Pokeweed Mitogens; D003208:Concanavalin A; D016753:Interleukin-10; D012431:Rutin; D007371:Interferon-gamma