Production of biosurfactant and antifungal compound by fermented food isolate Bacillus subtilis 20B.
A biosurfactant producing strain, Bacillus subtilis 20B, was isolated from fermented food in India. The strain also showed inhibition of various fungi in in-vitro experiments on Potato Dextrose Agar medium. It was capable of growth at temperature 55 degrees C and salts up to 7%. It utilized different sugars, alcohols, hydrocarbons and oil as a carbon source, with preference for sugars. In glucose based minimal medium it produced biosurfactant which reduced surface tension to 29.5 mN/m, interfacial tension to 4.5 mN/m and gave stable emulsion with crude oil and n-hexadecane. The biosurfactant activity was stable at high temperature, a wide range of pH and salt concentrations for five days. Oil displacement experiments using biosurfactant containing broth in sand pack columns with crude oil showed 30.22% recovery. The possible application of organism as biocontrol agent and use of biosurfactant in microbial enhanced oil recovery (MEOR) is discussed.
Joshi
Sanket
S
Department of Microbiology and Biotechnology Centre, The Maharaja Sayajirao University of Baroda, Vadodara 390 002, Gujarat, India.
Bharucha
Chirag
C
Desai
Anjana J
AJ
D000935:Antifungal Agents / Q000138:chemical synthesis*; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D019149:Bioreactors; D002244:Carbon / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation / Q000187:drug effects; D005516:Food Microbiology; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D017550:Spectroscopy, Fourier Transform Infrared; D013501:Surface-Active Agents / Q000138:chemical synthesis* / Q000302:isolation & purification
D000935:Antifungal Agents; D013501:Surface-Active Agents; D002244:Carbon