The Escherichia coli Yej transporter is required for the uptake of translation inhibitor microcin C.
Microcin C (McC), a peptide-nucleotide antibiotic, targets aspartyl-tRNA synthetase. By analyzing a random transposon library, we identified Escherichia coli mutants resistant to McC. Transposon insertions were localized to a single locus, yejABEF, which encodes components of a putative inner membrane ABC transporter. Analysis of site-specific mutants established that all four components of the transporter are required for McC sensitivity. Since aspartyl-tRNA synthetase in yej mutant extracts was fully sensitive to McC, we conclude that yej mutations interfere with McC uptake and that YejABEF is the only inner membrane transporter responsible for McC uptake in E. coli. Other substrates of YejABEF remain to be identified.
Novikova
Maria
M
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia.
Metlitskaya
Anastasia
A
Datsenko
Kirill
K
Kazakov
Teymur
T
Kazakov
Alexey
A
Wanner
Barry
B
Severinov
Konstantin
K
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D001430:Bacteriocins / Q000378:metabolism* / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D010802:Phylogeny; D014176:Protein Biosynthesis / Q000187:drug effects
D018528:ATP-Binding Cassette Transporters; D001430:Bacteriocins; D029968:Escherichia coli Proteins; C020411:microcin