title: The Escherichia coli Yej transporter is required for the uptake of translation inhibitor microcin C.
abstract: Microcin C (McC), a peptide-nucleotide antibiotic, targets aspartyl-tRNA synthetase. By analyzing a random transposon library, we identified Escherichia coli mutants resistant to McC. Transposon insertions were localized to a single locus, yejABEF, which encodes components of a putative inner membrane ABC transporter. Analysis of site-specific mutants established that all four components of the transporter are required for McC sensitivity. Since aspartyl-tRNA synthetase in yej mutant extracts was fully sensitive to McC, we conclude that yej mutations interfere with McC uptake and that YejABEF is the only inner membrane transporter responsible for McC uptake in E. coli. Other substrates of YejABEF remain to be identified.
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia.
authors.forename: Maria
authors.initials: M
authors.lastname: Novikova
authors.forename: Anastasia
authors.initials: A
authors.lastname: Metlitskaya
authors.forename: Kirill
authors.initials: K
authors.lastname: Datsenko
authors.forename: Teymur
authors.initials: T
authors.lastname: Kazakov
authors.forename: Alexey
authors.initials: A
authors.lastname: Kazakov
authors.forename: Barry
authors.initials: B
authors.lastname: Wanner
authors.forename: Konstantin
authors.initials: K
authors.lastname: Severinov
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D001430:Bacteriocins / Q000378:metabolism* / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D010802:Phylogeny; D014176:Protein Biosynthesis / Q000187:drug effects
chemical_list: D018528:ATP-Binding Cassette Transporters; D001430:Bacteriocins; D029968:Escherichia coli Proteins; C020411:microcin