Partitioning of caseinomacropeptide in aqueous two-phase systems.
This study evaluates the influence of type of salt and temperature on the partition coefficient of caseinomacropetide (CMP) to determine the best conditions for the recovery of CMP in aqueous two-phase systems (ATPS) composed by poly(ethylene glycol) (PEG) 1500 and an inorganic salt (potassium phosphate, sodium citrate, lithium sulfate or sodium sulfate). In all systems, CMP presented affinity for the PEG-rich phase. The PEG1500+lithium sulfate showed the highest values of partitioning coefficient. In addition, thermodynamic parameters (DeltaH degrees , DeltaS degrees , DeltaG degrees) as a function of temperature, were calculated for the system PEG1500-sodium citrate at different PEG concentrations and the results imply thermodynamic differences between partitioning of CMP in this system.
da Silva
César A Sodré
CA
Separation Process Laboratory (LPS), Department of Food Technology, Federal University of Viçosa (UFV), P. H. Rolfs Av., s/n, 36570-000 Viçosa, MG, Brazil.
Coimbra
Jane S R
JS
Rojas
Edwin E Garcia
EE
Minim
Luis A
LA
da Silva
Luis Henrique Mendes
LH
D002364:Caseins / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D010446:Peptide Fragments / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D012492:Salts / Q000737:chemistry; D013696:Temperature; D013816:Thermodynamics; D014867:Water / Q000737:chemistry
D002364:Caseins; D010446:Peptide Fragments; D012492:Salts; C065989:caseinomacropeptide; D014867:Water; D011092:Polyethylene Glycols