N-Methyl substituted 2',4'- BNANC: a highly nuclease-resistant nucleic acid analogue with high-affinity RNA selective hybridization.
Oligonucleotides modified with a novel BNA analogue, 2', 4'-BNA(NC)[N-Me], were synthesized, and in comparison to 2',4'-BNA (LNA), have similarly high RNA affinity, better RNA selectivity and much higher resistance to nuclease degradation, suggesting that the novel BNA analogue may be particularly useful for antisense approaches.
Miyashita
Kazuyuki
K
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan. imanishi@phs.osaka-u.ac.jp.
Rahman
S M Abdur
SM
Seki
Sayori
S
Obika
Satoshi
S
Imanishi
Takeshi
T
D001483:Base Sequence; D004720:Endonucleases / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis* / Q000187:drug effects / Q000494:pharmacology; D012313:RNA / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid
D016376:Oligonucleotides, Antisense; D012313:RNA; D004720:Endonucleases