Identification of the DNA sequence interacting with Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor 1.
Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiological agent of Kaposi's sarcoma. The open reading frame (K9) of KSHV encodes viral interferon regulatory factor 1 (vIRF1), which functions as a repressor of interferon-mediated signal transduction. The amino-terminal region of vIRF1 displays significant homology to the DNA-binding domain of cellular interferon regulatory factors, supporting the theory that the protein interacts with specific DNA sequences. Here, we identify the consensus sequence of vIRF1-binding sites from a pool of random oligonucleotides. Moreover, our data show that vIRF1 interacts with the K3:viral dihydrofolate reductase:viral interleukin 6 promoter region in the KSHV genome.
Park
Junsoo
J
Korea Basic Science Institute, Gwangju, Korea. junsoo@kaist.ac.kr
Lee
Myung-Shin
MS
Yoo
Seung-Min
SM
Jeong
Kwi Wan
KW
Lee
Daeyoup
D
Choe
Joonho
J
Seo
Taegun
T
D001483:Base Sequence; D001665:Binding Sites; D016384:Consensus Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D015967:Gene Expression Regulation, Viral; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D050835:Interferon Regulatory Factors / Q000378:metabolism*; D015850:Interleukin-6 / Q000235:genetics; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000378:metabolism*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D004279:DNA, Viral; D050835:Interferon Regulatory Factors; D015850:Interleukin-6; C456022:K3 protein, Kaposi's sarcoma-associated herpesvirus; D009841:Oligonucleotides; D012097:Repressor Proteins; D014764:Viral Proteins; C413486:viral interferon regulatory factors; D013762:Tetrahydrofolate Dehydrogenase