Siderotyping of fluorescent Pseudomonas: molecular mass determination by mass spectrometry as a powerful pyoverdine siderotyping method.
The numerous pyoverdines so far characterized as siderophores of fluorescent Pseudomonas could be usually differentiated one from each others by the two physico-chemical and physiological methods of siderotyping, i.e., siderophore-isoelectrofocusing and siderophore-mediated iron uptake. As shown in the present paper, the structural diversity of the peptide chain characterizing these molecules results in a very large panel of molecular masses representing 64 different values ranging from 889 to 1,764 Da for the 68 compounds included in the study, with only a few structurally different compounds presenting an identical molecular mass. Thus, the molecular mass determination of pyoverdines through mass spectrometry could be used as a powerful siderotyping method.
Meyer
Jean-Marie
JM
Département Génétique Moléculaire, Génomique, Microbiologie, UMR 7156, CNRS/Université Louis-Pasteur, 28 rue Goethe, 67000 Strasbourg, France. meyer@gem.u-strasbg.fr
Gruffaz
Christelle
C
Raharinosy
Vololoniaina
V
Bezverbnaya
Irina
I
Schäfer
Mathias
M
Budzikiewicz
Herbert
H
D005456:Fluorescent Dyes / Q000737:chemistry*; D007501:Iron / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D009842:Oligopeptides / Q000032:analysis* / Q000737:chemistry*; D011549:Pseudomonas / Q000737:chemistry*; D017262:Siderophores / Q000032:analysis* / Q000737:chemistry*
D005456:Fluorescent Dyes; D009842:Oligopeptides; D017262:Siderophores; C042453:pyoverdin; D007501:Iron