Development and validation of an accurate HPLC method for the quantitative determination of picroside II in tablets.
A simple, sensitive and accurate high performance liquid chromatographic method (HPLC) with UV detection was developed and validated to determine picroside II in a new tablet formulation with paeoniflorin as internal standard. Chromatographic separation was achieved on an Agilent XDB C18 column (250 x 4.6 mm I.D., 5 microm) using a mobile phase consisting of acetonitrile-water-acetic acid (18:82:0.4, v/v/v) at a flow rate of 1.0 ml/min. The UV detection wavelength was set at 265 nm. Linear calibration curves were obtained in the concentration range of 0.10-100 microg/ml with the limit of quantification (LOQ) of 0.10 microg/ml. The within- and between-run precisions in terms of % relative standard deviation (RSD) were lower than 5.7% and 6.3%, respectively. The accuracy in terms of % relative error (RE) ranged from -2.3% to 5.0%. This validated method was successfully applied to the determination of the content of picroside II in a new tablet formulation.
Cheng
Shan
S
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, Beijing, China. firhill@sina.com
Huang
Jia
J
He
Junqi
J
D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D007202:Indicators and Reagents; D057888:Iridoid Glucosides; D011786:Quality Control; D015203:Reproducibility of Results; D013607:Tablets
D002934:Cinnamates; D005960:Glucosides; D007202:Indicators and Reagents; D057888:Iridoid Glucosides; D013607:Tablets; C416837:picroside II