The narQP genes for a two-component regulatory system from the deep-sea bacterium Shewanella violacea DSS12.
Shewanella violacea DSS12 is facultative piezophile isolated from the deep-sea. The expression of cydDC genes (required for d-type cytochrome maturation) of the organism is regulated by hydrostatic pressure. In this study, we analyzed the nucleotide sequence upstream of cydDC in detail and found that there are putative binding sites for the NarL protein which is part of a two-component regulatory system also containing the sensor protein NarX. Furthermore, we identified the narQP genes (homologues of narXL) from S. violacea DSS12 and demonstrated the heterologous expression of narP in Escherichia coli. These results will be helpful in examining pressure regulation of gene expression in S. violacea at the molecular level.
Tamegai
Hideyuki
H
Department of Chemistry, College of Humanities and Sciences, Nihon University, Tokyo, Japan. htamegai@chs.nihon-u.ac.jp
Chikuma
Sayaka
S
Ishii
Masami
M
Nakasone
Kaoru
K
Kato
Chiaki
C
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D011994:Recombinant Proteins / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D016415:Sequence Alignment; D020592:Shewanella / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; D011994:Recombinant Proteins