title: Microarray analyses of differentially expressed human genes and biological processes in ECV304 cells infected with rubella virus.
abstract: Changes in mRNA expression levels of ECV304 cells infected with the wild-type rubella strain were analyzed using a microarray system representing 18,716 human genes. Four hundred eighty-seven genes exhibited differential expression levels; 456 of these genes were up-regulated while 31 genes were down-regulated. We identified 53 biological processes that were significantly relevant to the RV-infection. Among these biological processes, 52 were one-gene processes and one was a process involving five genes: IFNA21 (interferon, alpha 21), interferon stimulated exonuclease gene 20 kDa (ISG20), zinc finger protein 175 (ZNF175), tripartite motif-containing 22 (TRIM22), and MX2 [myxovirus (influenza virus) resistance 2 (mouse)]. Except for ZNF175, gene annotation indicated four of these genes encoded interferon or interferon-induced genes. These results suggest that genes relevant to interferon-regulated pathways may be involved in the pathogenesis of rubella.
authors.affiliation: Center for Heart Development, Key Lab of National Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan, China. moxiaoyang@yahoo.com.cn
authors.forename: Xiao-yang
authors.initials: XY
authors.lastname: Mo
authors.forename: Wenli
authors.initials: W
authors.lastname: Ma
authors.forename: Yali
authors.initials: Y
authors.lastname: Zhang
authors.forename: Haiquan
authors.initials: H
authors.lastname: Zhao
authors.forename: Yun
authors.initials: Y
authors.lastname: Deng
authors.forename: Wuzhou
authors.initials: W
authors.lastname: Yuan
authors.forename: Yuequn
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongqin
authors.initials: Y
authors.lastname: Li
authors.forename: Chuanbing
authors.initials: C
authors.lastname: Zhu
authors.forename: Mingyao
authors.initials: M
authors.lastname: Liu
authors.forename: Xiushan
authors.initials: X
authors.lastname: Wu
mesh_terms: D002460:Cell Line; D042783:Endothelial Cells / Q000821:virology*; D004730:Endothelium, Vascular / Q000166:cytology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012412:Rubella virus / Q000472:pathogenicity*; D014471:Umbilical Veins
chemical_list: D011506:Proteins