Involvement of polycomb-group genes in establishing HoxD temporal colinearity.
Temporal colinearity in mouse HoxD is dependent on repressive activity of sequences within the 5' end of the complex. We show that a 5-kb DNA fragment from this region represses transgenes when combined in mouse as well as in Drosophila melanogaster. Moreover, repressive activity in Drosophila depends on some members of the Polycomb-group (PcG) genes, for example, extra sex combs. We also showed direct association of these factors with the repressive fragment, both in transgenic flies and in the context of the native mouse HoxD complex. These results suggest that the global repressive region of the HoxD complex functions in two very different species and that some PcG genes are involved in establishing the early repressive state of the HoxD complex, thus contributing to temporal colinearity.
Mishra
Rakesh K
RK
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, India.
Yamagishi
Takumi
T
Vasanthi
D
D
Ohtsuka
Chiho
C
Kondo
Takashi
T
D000818:Animals; D030801:Animals, Genetically Modified; D019521:Body Patterning / Q000235:genetics*; D004330:Drosophila / Q000196:embryology / Q000235:genetics; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D051379:Mice; D008810:Mice, Inbred C57BL; D041681:NIH 3T3 Cells; D063151:Polycomb Repressive Complex 2; D063146:Polycomb-Group Proteins; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D019076:Transgenes
C107275:Eed protein, mouse; D063146:Polycomb-Group Proteins; D012097:Repressor Proteins; D063151:Polycomb Repressive Complex 2