Application of Plackett-Burman experimental design and Doehlert design to evaluate nutritional requirements for xylanase production by Alternaria mali ND-16.
The objective of this study was to use statistically based experimental designs for the optimization of xylanase production from Alternaria mali ND-16. Ten components in the medium were screened for nutritional requirements. Three nutritional components, including NH(4)Cl, urea, and MgSO(4), were identified to significantly affect the xylanase production by using the Plackett-Burman experimental design. These three major components were subsequently optimized using the Doehlert experimental design. By using response surface methodology and canonical analysis, the optimal concentrations for xylanase production were: NH(4)Cl 11.34 g L(-1), urea 1.26 g L(-1), and MgSO(4) 0.98 g L(-1). Under these optimal conditions, the xylanase activity from A. mali ND-16 reached 30.35 U mL(-1). Verification of the optimization showed that xylanase production of 31.26 U mL(-1) was achieved.
Li
Yin
Y
Department of Plant Science, North Dakota State University, Fargo, ND 58105, USA. yin.li@ndsu.edu
Liu
Zhiqiang
Z
Cui
Fengjie
F
Liu
Zhisheng
Z
Zhao
Hui
H
D000528:Alternaria / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D000643:Ammonium Chloride / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D005524:Food Technology / Q000379:methods; D008278:Magnesium Sulfate / Q000378:metabolism; D008969:Molecular Sequence Data; D012107:Research Design; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014508:Urea / Q000378:metabolism; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000096:biosynthesis* / Q000378:metabolism
D003470:Culture Media; D014990:Xylans; D000643:Ammonium Chloride; D008278:Magnesium Sulfate; D014508:Urea; D014995:Xylosidases; C026579:exo-1,4-beta-D-xylosidase