Determination of camptothecins in DMSO extracts of Nothapodytes foetida by direct injection capillary electrophoresis.
A rapid capillary electrophoresis procedure was developed for determining the anti-cancer components, camptothecins, in Nothapodytes foetida. The hydrophobic compound was extracted from plant tissue (ca. 1 mL of DMSO for 100 mg of dried plant tissue) with a water-miscible organic solvent, DMSO, at elevated temperature (60 degrees C). The extract was directly injected into the separation capillary (untreated fused silica, 34 cm in length, 75 microm i.d.) and analysed in MEKC mode (369 nm). Within 5 min of migration, camptothecins were successfully separated and quantified by adding organic modifiers to the running buffer (20% DMSO, 90 mm SDS in 10 mm borate buffer, pH 8.60). The linear dynamic range for camptothecin was from 5 to 400 microg/mL. This method was proven to be very suitable for monitoring the amount of camptothecins during the cultivation of the medicinal plant.
Hsiao
Hsien-Yi
HY
Department of Bio-Industrial Mechatronics Engineering, College of Bioresources and Agriculture, National Taiwan University, Taipei, Taiwan.
Cheng
Tzong-Jih
TJ
Yang
Ge-Ming
GM
Huang
I-Jen
IJ
Chen
Richie L C
RL
D002166:Camptothecin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D019684:Magnoliopsida / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet
D010936:Plant Extracts; D002166:Camptothecin; D000432:Methanol; D004121:Dimethyl Sulfoxide