Troponin T and histological characteristics of rat myocardial infarction induced by isoproterenol.
In our investigation,we used short-time model of myocardial infarction of rats induced by high dose of isoproterenol (ISP). We investigated cardiac troponin T blood level (cTnT) and histological characteristics of rat myocardium. ISP, single, intraperitoneal dose 250 mg/kg was given to male, adult, Wistar rats (n=12). Rats were distributed depending on their body weight in subgroups: ISP I (BW 260-280g) and ISP II (BW 250-400g).Control group (n=9) was treated with intraperitoneal dose of 0,95% NaCl. Cardiac TnT was measured by electrochemiluminiscence (ECLA) sandwich immunoassay in rat serum 4 hours after ISP application. Rats' hearts were dissected and examined by qualitative histological method (HE). Statistical significance was set at 0,05. There was significant difference in cTnT of ISP II (p=0,0001) vs. control and ISP I (p<0,05) vs. control. Significant difference was between ISP I and ISP II subgroups (p<0.001). The accent of histological changes of myocardium was on nuclei of cell. Cells showed acidophilic changes and nuclei disappearance as signs of coagulative necrosis development. Extensivity of histological changes were different between ISP I and ISP II subgroup. Used dose of ISP induced development of myocardial necrosis in rats. Subendocardial portion of myocardium was more vulnerability than subepicardial portion. Rats of ISP II had more extensive histological changes than these in ISP I. Administered doses of ISP enabled cTnT utilization as a marker of myocardial necrosis.
Hasić
Sabaheta
S
Institute for Physiology and Biochemistry, Faculty of Medicine, University of Sarajevo, Cekalusa 90, 71000 Sarajevo, Bosnia and Herzegovina.
Jadrić
Radivoj
R
Kiseljaković
Emina
E
Mornjaković
Zakira
Z
Winterhalter-Jadrić
Mira
M
D000818:Animals; D001835:Body Weight; D002316:Cardiotonic Agents / Q000494:pharmacology; D003402:Creatine Kinase / Q000378:metabolism; D006321:Heart / Q000502:physiology; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D009203:Myocardial Infarction / Q000139:chemically induced / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D009336:Necrosis; D051381:Rats; D017208:Rats, Wistar; D020107:Troponin T / Q000096:biosynthesis* / Q000502:physiology
D002316:Cardiotonic Agents; D020107:Troponin T; D003402:Creatine Kinase; D007545:Isoproterenol