RT-PCR-based cytochrome P450 expression profile of oral tissue samples.
OBJECTIVE
To analyse the expression of individual forms of cytochrome P450 (CYP) at the mRNA level in five homogenized oral buccal tissue samples from four individuals with or without oral malignancy.


METHODS
Individual forms of CYPs were studied by reverse transcriptase-polymerase chain reaction (RT-PCR), using specific primers for CYPs 2B6, 2C, 2D6, 2E1, 3A3/4 and 3A5, and oral CYP expressions were compared with CYP expression in liver tissue.


RESULTS
Consistent expression of CYPs 2C, 2E1 and 3A5 was observed in oral buccal tissue at mRNA level.


CONCLUSIONS
These particular CYPs have possible roles in the protection of the body against orally ingested xenobiotics as well as influence the bioavailability of therapeutic compounds.
Sarikaya
D
D
Department of Bioengineering, Faculty of Engineering, University of Hokkaido, Japan.
Chiba
I
I
Bilgen
C
C
Kamatakii
T
T
Topcu
Z
Z
D000368:Aged; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008099:Liver / Q000201:enzymology; D008875:Middle Aged; D009061:Mouth Mucosa / Q000201:enzymology*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007527:Isoenzymes; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System