title: Analysis of human papillomavirus and Epstein-Barr virus infection and aberrant death-associated protein kinase methylation in high-grade squamous intraepithelial lesions.
abstract: This study was conducted to investigate the presence of Epstein-Barr virus (EBV) and human papillomavirus (HPV) and the promoter methylation status of the death-associated protein kinase (DAPK) gene in high-grade intraepithelial lesions. Viral infection was analyzed using polymerase chain reaction (PCR), and promoter methylation status was evaluated using chemical modification by sodium bisulfite followed by PCR. A total of 24 samples were studied. HPV was detected in 16.6%, EBV in 16.6%, and HPV/EBV coinfection in 16.6%. No virus infection was detected in 50% of the samples studied. DAPK promoter methylation was observed in 29.2% of the analyzed samples. There was no significant correlation between DAPK methylation and viral infection. DAPK methylation was detected in 28% of HPV-positive lesions, in 28% of HPV- and EBV-positive lesions, and in 44% (3/7) of the samples without viral infection. There was no observed methylation in samples with isolated EBV infection. In DAPK unmethylated samples, HPV infection was found in 12%, EBV infection in 23%, HPV/EBV coinfection in 12%, and an absence of HPV and EBV infection in 53%. The promoter methylation of the DAPK gene is an important event during carcinogenesis and may have potential clinical application as a marker for the progression and prognosis of cancer.
authors.affiliation: Gene Expression Control Laboratory, Carlos Chagas Filho Institute of Biophysics and Institute of Gynecology, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. fernandalattario@gmail.com
authors.forename: F
authors.initials: F
authors.lastname: Lattario
authors.forename: Y L
authors.initials: YL
authors.lastname: Furtado
authors.forename: R
authors.initials: R
authors.lastname: Fonseca
authors.forename: F A
authors.initials: FA
authors.lastname: Silveira
authors.forename: I C
authors.initials: IC
authors.lastname: do Val
authors.forename: G
authors.initials: G
authors.lastname: Almeida
authors.forename: M G C
authors.initials: MG
authors.lastname: Carvalho
mesh_terms: D052685:Alphapapillomavirus / Q000235:genetics* / Q000302:isolation & purification; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D001483:Base Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology / Q000821:virology; D019175:DNA Methylation; D004279:DNA, Viral / Q000032:analysis*; D064550:Death-Associated Protein Kinases; D020031:Epstein-Barr Virus Infections / Q000453:epidemiology / Q000235:genetics / Q000821:virology; D005260:Female; D006367:HeLa Cells; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000235:genetics / Q000821:virology; D015995:Prevalence; D011401:Promoter Regions, Genetic; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology / Q000821:virology
chemical_list: D051017:Apoptosis Regulatory Proteins; D004279:DNA, Viral; D064550:Death-Associated Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases