title: Protein pyrophosphorylation by inositol pyrophosphates is a posttranslational event.
abstract: In a previous study, we showed that the inositol pyrophosphate diphosphoinositol pentakisphosphate (IP(7)) physiologically phosphorylates mammalian and yeast proteins. We now report that this phosphate transfer reflects pyrophosphorylation. Thus, proteins must be prephosphorylated by ATP to prime them for IP(7) phosphorylation. IP(7) phosphorylates synthetic phosphopeptides but not if their phosphates have been masked by methylation or pyrophosphorylation. Moreover, IP(7) phosphorylated peptides are more acid-labile and more resistant to phosphatases than ATP phosphorylated peptides, indicating a different type of phosphate bond. Pyrophosphorylation may represent a novel mode of signaling to proteins.
authors.affiliation: The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA.
authors.forename: Rashna
authors.initials: R
authors.lastname: Bhandari
authors.forename: Adolfo
authors.initials: A
authors.lastname: Saiardi
authors.forename: Yousef
authors.initials: Y
authors.lastname: Ahmadibeni
authors.forename: Adele M
authors.initials: AM
authors.lastname: Snowman
authors.forename: Adam C
authors.initials: AC
authors.lastname: Resnick
authors.forename: Troels Z
authors.initials: TZ
authors.lastname: Kristiansen
authors.forename: Henrik
authors.initials: H
authors.lastname: Molina
authors.forename: Akhilesh
authors.initials: A
authors.lastname: Pandey
authors.forename: J Kent
authors.initials: JK
authors.lastname: Werner
authors.forename: Krishna R
authors.initials: KR
authors.lastname: Juluri
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.forename: Glenn D
authors.initials: GD
authors.lastname: Prestwich
authors.forename: Keykavous
authors.initials: K
authors.lastname: Parang
authors.forename: Solomon H
authors.initials: SH
authors.lastname: Snyder
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D000595:Amino Acid Sequence; D011756:Diphosphates / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000737:chemistry; D007295:Inositol Phosphates / Q000737:chemistry*; D008745:Methylation; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
chemical_list: D011756:Diphosphates; D007295:Inositol Phosphates; D010455:Peptides; D010710:Phosphates; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate