title: Extra virgin olive oils increase hepatic fat accumulation and hepatic antioxidant protein levels in APOE-/- mice.
abstract: We assessed the effects of Picual and Arbequina olive oil, rich and poor in polyphenols, respectively, on plasma lipid and glucose metabolism, hepatic fat content, and the hepatic proteome in female Apoe-/- mice. Both olive oils increased hepatic fat content and adipophilin levels (p < 0.05), though Picual olive oil significantly decreased plasma triglycerides (p < 0.05). Proteomics identified a range of hepatic antioxidant enzymes that were differentially regulated by both olive oils as compared with palm oil. We found a clear association between olive oil consumption and differential regulation of adipophilin and betaine homocysteine methyl transferase as modulators of hepatic triglyceride metabolism. Therefore, our "systems biology" approach revealed hitherto unrecognized insights into the triglyceride-lowering and anti-atherogenic mechanisms of extra virgin olive oils, wherein the up-regulation of a large array of anti-oxidant enzymes may offer sufficient protection against lesion development and diminish oxidative stress levels instigated by hepatic steatosis.
authors.affiliation: Department of Biochemistry, Veterinary School, University of Zaragoza, Spain.
authors.forename: Jose Miguel
authors.initials: JM
authors.lastname: Arbones-Mainar
authors.forename: Karen
authors.initials: K
authors.lastname: Ross
authors.forename: Garry J
authors.initials: GJ
authors.lastname: Rucklidge
authors.forename: Martin
authors.initials: M
authors.lastname: Reid
authors.forename: Gary
authors.initials: G
authors.lastname: Duncan
authors.forename: John R
authors.initials: JR
authors.lastname: Arthur
authors.forename: Graham W
authors.initials: GW
authors.lastname: Horgan
authors.forename: Maria A
authors.initials: MA
authors.lastname: Navarro
authors.forename: Ricardo
authors.initials: R
authors.lastname: Carnicer
authors.forename: Carmen
authors.initials: C
authors.lastname: Arnal
authors.forename: Jesus
authors.initials: J
authors.lastname: Osada
authors.forename: Baukje
authors.initials: B
authors.lastname: de Roos
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001057:Apolipoproteins E / Q000235:genetics*; D015415:Biomarkers / Q000378:metabolism; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D004435:Eating; D005260:Female; D005419:Flavonoids / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D000069463:Olive Oil; D009929:Organ Size; D000070780:Perilipin-2; D010636:Phenols / Q000494:pharmacology; D010938:Plant Oils / Q000494:pharmacology*; D059808:Polyphenols; D025341:Principal Component Analysis; D020543:Proteome / Q000096:biosynthesis / Q000378:metabolism*; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism; D000094765:Glutathione Peroxidase GPX1
chemical_list: D000975:Antioxidants; D001057:Apolipoproteins E; D015415:Biomarkers; D004042:Dietary Fats, Unsaturated; D005419:Flavonoids; D008055:Lipids; D008565:Membrane Proteins; D000069463:Olive Oil; D000070780:Perilipin-2; D010636:Phenols; D010938:Plant Oils; C000602983:Plin2 protein, mouse; D059808:Polyphenols; D020543:Proteome; D005979:Glutathione Peroxidase; D013880:Thioredoxin-Disulfide Reductase; D005982:Glutathione Transferase; D000094765:Glutathione Peroxidase GPX1; C000722068:Gpx1 protein, mouse