Sensitive voltammetric determination of tyrosine using multi-walled carbon nanotubes/4-aminobenzeresulfonic acid film-coated glassy carbon electrode.
A chemically modified electrode is constructed based on the multi-walled carbon nanotubes (MWNTs)/4-aminobenzeresulfonic acid (4-ABSA) film-coated glassy carbon electrode. The electrocatalytic oxidation of tyrosine (Tyr) is investigated on the surface of the MWNTs/4-ABSA-modified electrode using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The prepared modified electrode shows voltammetric responses with high sensitivity and selectivity for Tyr in optimal conditions, which makes it very suitable for sub-micromolar detection of Tyr. A sensitive oxidation peak at +0.64 V is employed to determine Tyr. Good linear relationship between the oxidation peak current and the Tyr concentration in the range of 1x10(-7) to 5x10(-5) mol/L is obtained in phosphate buffer solution with pH 7.0. By use of modified electrode, the voltammetric detection limit for Tyr in DPV measurements is 8x10(-8) mol/L (S/N=3). Good sensitivity, selectivity and stability of the low-cost modified electrode make it very suitable for the determination of trace amounts of Tyr in pharmaceutical and clinical preparations.
Huang
Ke-Jing
KJ
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, PR China. kejinghuang@163.com
Luo
Ding-Fa
DF
Xie
Wan-Zhen
WZ
Yu
Yong-Sheng
YS
D002138:Calibration; D004563:Electrochemistry / Q000379:methods*; D005898:Glass / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction; D004364:Pharmaceutical Preparations / Q000737:chemistry; D015203:Reproducibility of Results; D013425:Sulfanilic Acids / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry*; D013997:Time Factors; D014443:Tyrosine / Q000032:analysis*
D037742:Nanotubes, Carbon; D004364:Pharmaceutical Preparations; D013425:Sulfanilic Acids; D013451:Sulfonic Acids; D014443:Tyrosine; C100016:4-sulfanilic acid