Genetic diagnosis of multiple affected tissues in a patient with McCune-Albright syndrome.
McCune-Albright syndrome (MAS) is a sporadic disorder characterized by the classic triad of polyostotic fibrous dysplasia, café-au-lait' skin pigmentation, and hyperfunctional endocrinopathy. It is caused by embryonic somatic mutations leading to the substitution of His or Cys for Arg at amino acid 201 of the alpha-subunit of the signal transduction protein Gs (Gsalpha). A 32-year-old man was diagnosed as McCune-Albright syndrome with the following findings: polyostotic fibrous dysplasia, café-au-lait' spots and acromegaly. An ultrasonic examination showed that he had left-pleural effusion, which disappeared after almost a year without special treatment. Genomic DNA was isolated from the peripheral blood, bone tissue, skin lesion and pleura samples of the patient. Then PCR and direct sequencing were performed. An activating mutation of the Gsalpha gene (Arg201Cys) was found in the genomic DNA isolated from the peripheral blood and the bone tissue, but not in genomic DNA isolated from the skin and pleura samples.
Zhou
Ji
J
Department of Endocrinology and Metabolic Diseases, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin Er Road, Shanghai, 200025, China.
Sun
Li-hao
LH
Cui
Bin
B
Song
Huai-dong
HD
Li
Xiao-ying
XY
Ning
Guang
G
Liu
Jian-min
JM
D000172:Acromegaly / Q000150:complications / Q000235:genetics; D000328:Adult; D001483:Base Sequence; D019080:Cafe-au-Lait Spots / Q000150:complications / Q000473:pathology; D004252:DNA Mutational Analysis; D005359:Fibrous Dysplasia, Polyostotic / Q000150:complications / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology*; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000235:genetics; D006801:Humans; D008297:Male; D009154:Mutation; D020858:Tissue and Organ Harvesting
D019205:GTP-Binding Protein alpha Subunits, Gs