High-resolution in vivo imaging of blood vessels without labeling.
We demonstrate that both oxyhemoglobin and deoxyhemoglobin have sequential two-color, two-photon absorption properties that can serve as endogenous contrasts in microvasculature imaging. Using a sensitive modulation transfer technique, we are able to image hemoglobin in red blood cells with micrometer resolution, both in vitro and in vivo. We show that excellent contrast from hemoglobin without any labeling can be obtained in tissue.
Fu
Dan
D
Department of Chemistry, Princeton University, Princeton, NJ 08544, USA. dfu@princeton.edu
Ye
Tong
T
Matthews
Thomas E
TE
Chen
Benny J
BJ
Yurtserver
Gunay
G
Warren
Warren S
WS
D000818:Animals; D001808:Blood Vessels / Q000166:cytology* / Q000378:metabolism*; D002478:Cells, Cultured; D004912:Erythrocytes / Q000166:cytology* / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D013194:Staining and Labeling
D006454:Hemoglobins