title: Schizosaccharomyces pombe nup97, which Genetically Interacts with mex67, is essential for growth and involved in mRNA export.
abstract: We have isolated previously three synthetic lethal mutants in Schizosaccharomyces pombe, which genetically interact with mex67, in order to identify the genes involved in mRNA export. A novel nup97 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMex3. The nup97 gene contains one intron and encodes an 851 amino-acid protein that is similar to nucleoporins, Npp106p in S. pombe and Nic96p in Saccharomyces cerevisiae. The nup97 gene is essential for vegetative growth, and nup97 null mutant harboring pREP41X-Nup97 showed poly(A)+ RNA export defect when expression of nup97 is repressed in the presence of thiamine. These results suggest that nup97 is involved in mRNA export from the nucleus to cytoplasm.
authors.affiliation: Deptartment of Biology, Institute of Basic Sciences, Sungshin Women's University, Seoul 136-742, Republic of Korea.
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Cho
authors.forename: Duk Kyung
authors.initials: DK
authors.lastname: Hwang
authors.forename: Sun Im
authors.initials: SI
authors.lastname: Jung
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D020043:Genes, Essential; D005800:Genes, Fungal / Q000235:genetics* / Q000502:physiology; D005816:Genetic Complementation Test; D008957:Models, Genetic; D009154:Mutation; D034443:RNA Transport; D012331:RNA, Fungal / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D012331:RNA, Fungal; D012333:RNA, Messenger