Quantitative assessment of transition proteins 1, 2 spermatid-specific linker histone H1-like protein transcripts in spermatozoa from normozoospermic and asthenozoospermic men.
Spermatid-specific linker histone H1-like protein (HILS1), transition proteins 1 and 2 (TNP1 and TNP2), and protamines 1 and 2 (PRM1 and PRM2) contribute to considerable dense packing of spermatid chromatin during spermiogenesis. We evaluated the HILS1, TNP1, and TNP2 transcript levels in spermatozoa isolated from normozoospermic and asthenozoospermic men. Human ejaculates from normozoospermic (n = 70) and asthenozoospermic (n = 100) donors were purified by centrifugation through a discontinuous Percoll density gradient. RNA was isolated from spermatozoa according to the Chomczynski and Sacchi method, treated with DNase I and reverse-transcribed into cDNA. Quantitative analysis of HILS1, TNP1, and TNP2 transcripts was performed by real-time quantitative (RQ-PCR) SYBR green I analysis. We found significantly lower levels of HILS1, TNP1, and TNP2 transcripts in spermatozoa from asthenozoospermic men compared to normozoospermic men. Our observations suggest that a reduction in HILS1, TNP1, and TNP2 transcripts may be associated with asthenozoospermia.
Jedrzejczak
Piotr
P
Department of Infertility and Reproductive Endocrinology, Poznań University of Medical Sciences, 6 Swiecickiego St., 60-781 Poznań, Poland.
Kempisty
Bartosz
B
Bryja
Artur
A
Mostowska
M
M
Depa-Martynow
Magdalena
M
Pawelczyk
Leszek
L
Jagodzinski
Pawel Piotr
PP
D053627:Asthenozoospermia / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D006801:Humans; D008297:Male; D011479:Protamines / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000378:metabolism*
D002868:Chromosomal Proteins, Non-Histone; C082073:PRM1 protein, human; D011479:Protamines; C068621:protamine 2; C053886:spermatid transition proteins