Aptameric enzyme subunit for homogeneous DNA sensing.
We have developed an aptameric enzyme subunit (AES) which can detect the DNA in a homogeneous solution. The AES is an artificial enzyme subunit composed of an enzyme-inhibiting aptamer bearing a target-molecule binding site. We connected a probe DNA to a thrombin-inhibiting aptamer at its 5' or 3' end. The inhibitory activity of the thrombin-inhibiting aptamer bearing the probe DNA decreased compared to that of the original aptamer; however, it recovered upon hybridization with the target DNA. Using this AES, we were able to detect target DNAs by measuring the thrombin activity in a homogeneous solution.
Ikebukuro
Kazunori
K
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan. ikebu@cc.tuat.ac.jp
Yoshida
Wataru
W
Sode
Koji
K
D052157:Aptamers, Nucleotide / Q000494:pharmacology; D001483:Base Sequence; D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D021122:Protein Subunits / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D013917:Thrombin / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000235:genetics
D052157:Aptamers, Nucleotide; D021122:Protein Subunits; D004247:DNA; D013917:Thrombin