title: Bioactive glass-derived hydroxyapatite-coating promotes granulation tissue growth in subcutaneous cellulose implants in rats.
abstract: Granulation tissue was induced in hydroxyapatite-coated cellulose sponges with subcutaneous implantation in rats. A massive inflammatory reaction with an intense foreign body reaction and an increased invasion of fibrovascular tissue was observed by days 1-3 post-operation, whereas tissue growth into the uncoated control implants was much slower and took place mainly on their surfaces. The foreign body reaction in apatite-coated sponges declined after post-operative day 14, and no obvious differences were seen between the two cellulose sponges from 1 month up to 1 year after implantation. The apatite-coated implants attracted macrophages and fibroblasts, and favored angiogenesis. The excessive connective tissue formation was histologically normal, synthesized the major extracellular matrix molecules in a normal ratio and did not seem to disturb the animals in any way. These results warrant further investigations on clinical applicability of hydroxyapatite-coated cellulose sponges, when fast proliferation of connective tissue is desirable.
authors.affiliation: Departments of Medical Biochemistry and Molecular Biology, University of Turku, Kiinamyllynkatu 10, FIN-20520 Turku, Finland.
authors.forename: Miretta
authors.initials: M
authors.lastname: Tommila
authors.forename: Jutta
authors.initials: J
authors.lastname: Jokinen
authors.forename: Timothy
authors.initials: T
authors.lastname: Wilson
authors.forename: A-P
authors.initials: AP
authors.lastname: Forsback
authors.forename: Pekka
authors.initials: P
authors.lastname: Saukko
authors.forename: Risto
authors.initials: R
authors.lastname: Penttinen
authors.forename: Erika
authors.initials: E
authors.lastname: Ekholm
mesh_terms: D000818:Animals; D002482:Cellulose; D020099:Coated Materials, Biocompatible; D017886:Durapatite; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005898:Glass; D006097:Granulation Tissue / Q000254:growth & development* / Q000378:metabolism; D020007:Implants, Experimental; D008264:Macrophages / Q000166:cytology; D008297:Male; D008422:Materials Testing; D018919:Neovascularization, Physiologic; D012313:RNA / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015503:Surgical Sponges; D014945:Wound Healing
chemical_list: D020099:Coated Materials, Biocompatible; D016326:Extracellular Matrix Proteins; C403922:bioactive glass S53P4; D012313:RNA; D002482:Cellulose; D017886:Durapatite