title: Gene expression profiling of the rat endometriosis model.
abstract: OBJECTIVE
  To investigate the molecular mechanism of endometriosis, gene expression profiling was analyzed in a rat endometriosis model.
  
  
  METHODS
  An endometriosis model was induced by uterine autotransplantation in the peritoneal cavity on a female-SD rat (8 weeks old). As control samples, the normal uterine tissues were used. The gene expression was compared between endometriotic lesions and normal uterine tissues by cDNA microarray analysis, quantitative real time RT-PCR and immunohistochemistry.
  
  
  RESULTS
  The expression of 71 genes was upregulated and that of 45 genes was downregulated in the endometriotic lesions compared to normal uterine tissues. The upregulated genes included genes encoding cytokines, chemokines, growth factors and cell adhesion molecules. The levels of transcripts of osteopontin, Lyn, Vav1, Runx1, and l-selectin in the endometriotic lesions were 130, 10, 10, 12 and 46-fold higher than the respective levels in the eutopic endometrial samples.
  
  
  CONCLUSIONS
  The results suggest that osteopontin, Lyn, Vav1, Runx1, and l-selectin play important roles in the pathogenesis of endometriosis.
authors.affiliation: Department of Gynecology, Omiya Medical Center Jichi Medical University, Amanuma-cho, Omiya, Saitama, Japan. kryo77@excite.co.jp
authors.forename: Ryo
authors.initials: R
authors.lastname: Konno
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Fujiwara
authors.forename: Sachiho
authors.initials: S
authors.lastname: Netsu
authors.forename: Kohei
authors.initials: K
authors.lastname: Odagiri
authors.forename: Miyuki
authors.initials: M
authors.lastname: Shimane
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nomura
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D019041:L-Selectin / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D051567:Proto-Oncogene Proteins c-vav / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019061:src-Family Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; D051567:Proto-Oncogene Proteins c-vav; D012333:RNA, Messenger; C493727:Runx1 protein, rat; C506897:Spp1 protein, rat; C496620:Vav1 protein, rat; D053495:Osteopontin; D019041:L-Selectin; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases