title: Isolation and culture of different epidermal and dermal cell types from human scalp suitable for the development of a therapeutical cell spray.
abstract: BACKGROUND
  Previous studies demonstrated, that cultured epithelial autografts (CEA) can be isolated and skin cell sprays can be produced for application on different types of wounds. The purpose of the present study was to determine which cell types can be isolated from the human scalp and whether these cells can be used for spray transplantation.
  
  
  METHODS
  Outer root sheath cells (ORS), keratinocytes, melanocytes, dermal papilla cells (DP), and dermal sheath cells (DSC) were isolated from human scalp tissue. Isolated cells were characterized, expanded and sprayed in an in vitro model. Growth behaviour, morphology and cell counts were compared with non-sprayed cells.
  
  
  RESULTS
  With acceptable time, equipment and laboratory personnel a sufficient amount of keratinocytes, ORS, melanocytes, DP cells and DSC cells could be achieved. The cells are sufficient for application as a cell spray. Cells, positive for Integrin alpha6, Cytokeratin 19, CD73 and CD105 were identified within the cultures.
  
  
  CONCLUSIONS
  Human scalp is suitable to gain epidermal and dermal cells for the development of therapeutic cell spray transplantation. Further studies have to determine, whether these cells can be combined to produce wound specific skin substitutes.
authors.affiliation: Charité, Campus Virchow-Clinic, Department of Surgery, University Medicine, Berlin, Germany. juergen_schlabe@t-online.de
authors.forename: Juergen
authors.initials: J
authors.lastname: Schlabe
authors.forename: Christa
authors.initials: C
authors.lastname: Johnen
authors.forename: Ruth
authors.initials: R
authors.lastname: Schwartlander
authors.forename: Viola
authors.initials: V
authors.lastname: Moser
authors.forename: Bernd
authors.initials: B
authors.lastname: Hartmann
authors.forename: Jörg C
authors.initials: JC
authors.lastname: Gerlach
authors.forename: Markus V
authors.initials: MV
authors.lastname: Küntscher
mesh_terms: D000328:Adult; D000336:Aerosols; D000368:Aged; D001706:Biopsy / Q000379:methods; D018929:Cell Culture Techniques; D000078404:Epidermal Cells; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000637:transplantation; D008297:Male; D008544:Melanocytes / Q000166:cytology / Q000637:transplantation; D008875:Middle Aged; D012535:Scalp / Q000166:cytology*; D016038:Skin Transplantation / Q000379:methods*
chemical_list: D000336:Aerosols