Interactions of cell penetrating peptide Tat with model membranes: a biophysical study.
The protein transduction domain of the HIV-1 transactivator of transcription, Tat (Tat((48-60))), has been shown to transport P10, a cytotoxic peptide mimic of the cyclin dependent kinase inhibitor p21WAF1/CIP1, into the nucleus of cancerous cells and induce apoptosis. Here, monolayer studies were used to investigate the membrane interactions of Tat((48-60)), P10 and the construct Tat((48-60))P10. It was found that Tat((48-60)) showed no significant surface activity but that both P10 and Tat((48-60))P10, were highly surface active, inducing surface pressure changes of 9.7 and 8.9mNm(-1), respectively, with DMPS monolayers. The comparison of Tat((48-60))P10 and P10 surface interactions would be consistent with a hypothesis that the cargo attachment influences the capacity of the Tat-protein transduction domain to mediate transport across membranes either directly or via localisation of the construct at the membrane interface.
Dennison
Sarah R
SR
Faculty of Science and Technology, University of Central Lancashire, Preston PR1 2HE, UK.
Baker
Rachael D
RD
Nicholl
Iain D
ID
Phoenix
David A
DA
D001665:Binding Sites; D032701:Biomimetics / Q000379:methods*; D001703:Biophysics / Q000379:methods; D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D008567:Membranes, Artificial; D010446:Peptide Fragments / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D011485:Protein Binding; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000737:chemistry*
D008051:Lipid Bilayers; D008567:Membranes, Artificial; D010446:Peptide Fragments; D010743:Phospholipids; D054322:tat Gene Products, Human Immunodeficiency Virus; C422951:tat peptide (48-60), Human immunodeficiency virus 1