title: An effective method for the in situ synthesis of DNA-CPG conjugates using chemical ligation technology as tools for SNP analysis.
abstract: In this paper, we report a new method for the SNP analysis by using a chemical ligation (CL) technique on CPG plates with high coupling efficiency. This method showed markedly high match/mismatch discrimination ability. Particularly, replacement of thymidine with 2-thiothymidine in DNA probes used in the CL technology resulted in significant improvement of the base discrimination ability of the thymine base in this system.
authors.affiliation: 4259, Nagatsuta, Department of Life Science, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohkubo
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Tanaka
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Taguchi
authors.forename: Kohji
authors.initials: K
authors.lastname: Seio
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nagasawa
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Tsukahara
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
mesh_terms: D002851:Chromatography, High Pressure Liquid; D018899:CpG Islands; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D020641:Polymorphism, Single Nucleotide
chemical_list: D015342:DNA Probes; D004247:DNA