title: Antigen crosspresentation by human plasmacytoid dendritic cells.
abstract: Crosspresentation is a specialized function of myeloid dendritic cells (mDCs), allowing them to induce CD8+ T cell responses against exogenous antigens that are not directly produced in their cytotosol. Human plasmacytoid DCs (pDCs) are not considered so far as able to perform crosspresentation. We showed here that purified human pDCs crosspresented vaccinal lipopeptides and HIV-1 antigens from apoptotic cells to specific CD8+ T lymphocytes. Apoptotic debris were internalized by phagocytosis and the lipopeptide LPPol reached nonacidic endosomes. This crosspresentation was amplified upon influenza virus infection. Importantly, the efficiency of crosspresentation by pDCs was comparable to that of mDCs. This property of human pDCs needs to be taken into account to understand the pathogenesis of infectious, allergic, or autimmune diseases and to help achieve desired responses during vaccination by targeting specifically either type of DCs.
authors.affiliation: Département d'Immunologie, Institut Cochin, Paris, F-75014, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Hoeffel
authors.forename: Anne-Claire
authors.initials: AC
authors.lastname: Ripoche
authors.forename: Diana
authors.initials: D
authors.lastname: Matheoud
authors.forename: Michelina
authors.initials: M
authors.lastname: Nascimbeni
authors.forename: Nicolas
authors.initials: N
authors.lastname: Escriou
authors.forename: Pierre
authors.initials: P
authors.lastname: Lebon
authors.forename: Farhad
authors.initials: F
authors.lastname: Heshmati
authors.forename: Jean-Gérard
authors.initials: JG
authors.lastname: Guillet
authors.forename: Monique
authors.initials: M
authors.lastname: Gannagé
authors.forename: Sophie
authors.initials: S
authors.lastname: Caillat-Zucman
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Casartelli
authors.forename: Olivier
authors.initials: O
authors.lastname: Schwartz
authors.forename: Henri
authors.initials: H
authors.lastname: De la Salle
authors.forename: Daniel
authors.initials: D
authors.lastname: Hanau
authors.forename: Anne
authors.initials: A
authors.lastname: Hosmalin
authors.forename: Concepción
authors.initials: C
authors.lastname: Marañón
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D017951:Antigen Presentation / Q000276:immunology*; D017209:Apoptosis / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D006678:HIV / Q000276:immunology; D015488:HIV Antigens / Q000276:immunology*; D006801:Humans; D007962:Leukocytes / Q000276:immunology / Q000821:virology; D008074:Lipoproteins / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D018613:Microscopy, Confocal; D014162:Transfection
chemical_list: D016915:AIDS Vaccines; D015488:HIV Antigens; D008074:Lipoproteins