A high-resolution atlas of nucleosome occupancy in yeast.
We present the first complete high-resolution map of nucleosome occupancy across the whole Saccharomyces cerevisiae genome, identifying over 70,000 positioned nucleosomes occupying 81% of the genome. On a genome-wide scale, the persistent nucleosome-depleted region identified previously in a subset of genes demarcates the transcription start site. Both nucleosome occupancy signatures and overall occupancy correlate with transcript abundance and transcription rate. In addition, functionally related genes can be clustered on the basis of the nucleosome occupancy patterns observed at their promoters. A quantitative model of nucleosome occupancy indicates that DNA structural features may account for much of the global nucleosome occupancy.
Lee
William
W
Department of Genetics, Stanford University School of Medicine, Stanford, California 94305-5120, USA.
Tillo
Desiree
D
Bray
Nicolas
N
Morse
Randall H
RH
Davis
Ronald W
RW
Hughes
Timothy R
TR
Nislow
Corey
C
D016681:Genome, Fungal; D009707:Nucleosomes / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins