Expression and distribution of fugu TIMP-2s (fgTIMP-2a and fgTIMP-2b) mRNAs in tissues and embryos.
In teleosts, two distinct types of TIMP-2s occur, TIMP-2a and TIMP-2b, but little is known about their locations and quantitative expressions. Here, we examined pufferfish (Takifugu rubripes) TIMP-2a (fgTIMP-2a) and TIMP-2b (fgTIMP-2b) quantities and locations in fugu adult tissues and embryos. To compare the quantitative expression of fgTIMP-2s, we performed a quantitative real-time PCR (qPCR). FgTIMP-2a mRNA was constitutively expressed and significant differences in expression were not observed among adult tissues. Whereas, fgTIMP-2b mRNA was significantly differently expressed in ordinary muscle and gill compared to the expression level in whole blood (P<0.05). Although significant difference was not observed between brain and other tissues, both fgTIMP-2s mRNAs were abundant in the brain. In addition, we examined embryos during development using qPCR. Both fgTIMP-2s mRNAs gradually increased during embryonic development from 48 hpf. However, fgTIMP-2b mRNA was obviously abundant compared to fgTIMP-2a mRNA in embryos. We also examined the specific mRNA distribution in embryos. The fgTIMP-2s mRNAs showed the same distribution during development. Both fgTIMP-2s are expressed in adult fugu tissues and embryos but their expression levels clearly differ, suggesting that there is a predominance of fgTIMP-2b over fgTIMP-2a in vivo.
Tsukamoto
Hiroshi
H
Laboratory of Food Chemistry, Department of Marine Bioscience, Faculty of Biotechnology, Fukui Prefectural University, Obama, Fukui 917-0003, Japan.
Yokoyama
Yoshihiro
Y
Suzuki
Tohru
T
Mizuta
Shoshi
S
Yoshinaka
Reiji
R
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D047108:Embryonic Development; D017403:In Situ Hybridization; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D023741:Takifugu; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics*
D017931:DNA Primers; D020033:Protein Isoforms; D012333:RNA, Messenger; D019716:Tissue Inhibitor of Metalloproteinase-2