Chemical probes shed light on protein function.
Site-specific protein labeling with synthetic dyes is an emerging technique for live cell imaging. A protein or peptide tag fused to the protein of interest provides the means for attachment of a fluorophore or other small molecule probe, to allow non-invasive imaging of the dynamics of protein localization. The past two years have seen significant advances in such methods, the publication of a number of new tags for labeling, and the imaginative application of established techniques to tackle previously intractable biological questions.
O'Hare
Helen M
HM
Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.
Johnsson
Kai
K
Gautier
Arnaud
A
D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008958:Models, Molecular; D015335:Molecular Probes / Q000737:chemistry*; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000032:analysis* / Q000737:chemistry* / Q000502:physiology
D005456:Fluorescent Dyes; D015335:Molecular Probes; D011506:Proteins