Inhibition of transcription at radiation-induced nuclear foci of phosphorylated histone H2AX in mammalian cells.
Double-strand DNA breaks (DSBs) induced by ionizing radiation can be visualized in human cells using antibodies against Ser-139 phosphorylated histone H2AX (gamma-H2AX). Large gamma-H2AX foci are seen in the nucleus fixed 1 hour after irradiation and their number corresponds to the number of DSBs, allowing analysis of these genome lesions after low doses. We estimated whether transcription is affected in chromatin domains containing gamma-H2AX by following in vivo incorporation of 5-bromouridine triphosphate (BrUTP) loaded by cell scratching (run-on assay). We found that BrUTP incorporation is strongly suppressed at gamma-H2AX foci, suggesting that H2AX phosphorylation inhibits transcription. This is not caused by preferential association of gamma-H2AX foci with constitutive or facultative heterochromatin, which was visualized in irradiated cells using antibodies against histone H3 trimethylated at lysine-9 (H3-K9m3) or histone H3 trimethylated at lysine-27 (H3-K27m3). Apparently, formation of gamma-H2AX induces changes of chromatin that inhibit assembly of transcription complexes without heterochromatin formation. Inhibition of transcription by phosphorylation of histone H2AX can decrease chromatin movement at DSBs and frequency of misjoining of DNA ends.
Solovjeva
Liudmila V
LV
Institute of Cytology, Russian Academy of Sciences, Tikchoretskii Av. 4, 194064, St. Petersburg, Russia.
Svetlova
Maria P
MP
Chagin
Vadim O
VO
Tomilin
Nikolai V
NV
D001973:Bromodeoxyuridine / Q000494:pharmacology; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D019175:DNA Methylation; D003856:Deoxyuracil Nucleotides / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D018613:Microscopy, Confocal; D010766:Phosphorylation; D014158:Transcription, Genetic
D003856:Deoxyuracil Nucleotides; C468783:H2AX protein, human; D006657:Histones; C032549:bromodeoxyuridine triphosphate; D001973:Bromodeoxyuridine