title: A mev-1-like dominant-negative SdhC increases oxidative stress and reduces lifespan in Drosophila.
abstract: Oxidative stress is thought to promote aging by damaging various cellular components. We investigated the phenotypic effects of mutation in the sdhC gene encoding succinate dehydrogenase C in Drosophila by using transgenic flies expressing a dominant-negative form, SdhC(I71E). Expression of SdhC(I71E) significantly reduced the mean lifespan by 22% compared to that of control flies. The amount of protein carbonyl was significantly increased, suggesting that a high level of oxidative stress was induced in these flies. The transgenic flies should serve as a model for studying the biological processes associated with increased levels of oxidative stress and mitochondrial dysfunction in Drosophila.
authors.affiliation: Department of Biological Sciences, Tokyo Metropolitan University, 1-1 Minami-osawa, Hachioji-shi, Tokyo 192-0397, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Tsuda
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Sugiura
authors.forename: Takamasa
authors.initials: T
authors.lastname: Ishii
authors.forename: Naoaki
authors.initials: N
authors.lastname: Ishii
authors.forename: Toshiro
authors.initials: T
authors.lastname: Aigaki
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D045303:Cytochromes b; D004330:Drosophila / Q000502:physiology*; D008136:Longevity / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology*; D013385:Succinate Dehydrogenase / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D029742:Caenorhabditis elegans Proteins; C472666:MEV-1 protein, C elegans; C486355:SdhC protein, bacteria; D045303:Cytochromes b; D013385:Succinate Dehydrogenase