[Content of somatostatin and cholecystokinin-8 in hypothalamus and colons in a rat model of spleen-deficiency syndrome].
OBJECTIVE
To investigate the changes of neuropeptides content in a rat model of spleen-deficiency syndrome.


METHODS
Rats were randomly divided into three groups: normal group, untreated group and treatment group. There were ten rats in each group. Spleen-deficiency syndrome was induced by intragastrically administration of rhubarb concentrate in rats of the untreated and treatment group. The rats in the treatment group were intragastrically administered with Sijunzi decoction, a traditional Chinese compound herbal medicine for strengthening spleen and supporting qi. Normal saline was intragastrically administered to the rats in the normal and untreated groups. The somatostatin (SS) and cholecystokinin-8 (CCK-8) content in hypothalamus and colons was detected by the method of radioimmunoassay and the D-xylose content in urine was also detected.


RESULTS
The CCK-8 content in hypothalamus of the untreated group was higher than that of the normal group (P<0.05). SS and CCK-8 content in colons of the untreated group was higher than that of the normal group (P<0.05). The CCK-8 content in colons of the untreated group was lower than that of the treatment group (P<0.05). The excretion rate of D-xylose in the untreated group was lower than that of the normal group (P<0.05).


CONCLUSIONS
The disorder of neuroendocrine regulation in rats with spleen-deficiency syndrome may be chiefly responsible for "spleen-deficency and dysfunction of spleen" in traditional Chinese medicine.
Liu
Qun
Q
Department of Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China. liuqund@sina.com
Cai
Gan
G
D000818:Animals; D002766:Cholecystokinin / Q000378:metabolism*; D003106:Colon / Q000378:metabolism; D003937:Diagnosis, Differential; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D008516:Medicine, Chinese Traditional; D010446:Peptide Fragments / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000378:metabolism*; D013158:Splenic Diseases / Q000188:drug therapy / Q000378:metabolism; D016711:Yang Deficiency / Q000188:drug therapy / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D010446:Peptide Fragments; C410885:cholecystokinin 8; C403259:si-jun-zi-tang; D013004:Somatostatin; D002766:Cholecystokinin