Regulation of Saccharomyces cerevisiae DNA polymerase eta transcript and protein.
RAD30-encoded DNA polymerase eta functions as a translesion polymerase that can bypass the most frequent types of UV-induced pyrimidine photoproducts in an error-free manner. Although its transcript is UV-inducible in Saccharomyces cerevisiae, Rad30 (studied as a Rad30-Myc fusion) is a stable protein whose levels do not fluctuate following UV treatment or during cell cycle progression. Rad30 protein is subject to monoubiquitination whose level is upregulated in G1 and downregulated during S-phase reentry. This downregulation is accelerated in UV-treated cells. A missense mutation (L577Q) of the ubiquitin binding domain (UBZ) confers a reduced degree of ubiquitination outside of G1 and a complete failure to stably interact with ubiquitinated substrates. This mutation confers a phenotype resembling a complete RAD30 deletion, thus attesting to the significance of the UBZ motif for polymerase eta function in vivo.
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Ritu
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Department of Cell Biology and Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, TX 76107, USA.
Rozario
Donald
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Siede
Wolfram
W
D020816:Amino Acid Motifs; D004249:DNA Damage / Q000528:radiation effects; D004260:DNA Repair / Q000502:physiology / Q000528:radiation effects; D004261:DNA Replication / Q000502:physiology / Q000528:radiation effects; D004259:DNA-Directed DNA Polymerase / Q000096:biosynthesis* / Q000528:radiation effects*; D020536:Enzyme Activators; D005800:Genes, Fungal / Q000528:radiation effects; D012331:RNA, Fungal / Q000032:analysis / Q000528:radiation effects; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000528:radiation effects; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics / Q000528:radiation effects; D054875:Ubiquitination / Q000502:physiology*; D014466:Ultraviolet Rays / Q000009:adverse effects; D000099270:Y-Family DNA Polymerases
D004259:DNA-Directed DNA Polymerase; D020536:Enzyme Activators; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D044763:Ubiquitin-Conjugating Enzymes; C112338:Rad30 protein; D000099270:Y-Family DNA Polymerases