title: Antifibrotic effect via the regulation of transcription factor Sp1 in lung fibrosis.
abstract: The aim of this study is to evaluate the antifibrotic effect of ring-type Sp1 decoy oligonucleotides (ODNs) through blocking the transcription of transforming growth factor (TGF)-beta1 and its downstream target genes. In this experiment, the expression of TGF-beta1, metalloproteinase (MMP)-13, and fibronectin was decreased in the group with the treatment of the ring-type Sp1 decoy ODNs. Also, alpha-smooth muscle actin positive bronchial lining cells and alveolar epithelial cells were observed, especially around the lesions of extracellular matrix (ECM) deposition. These findings provide evidences for the finding of pulmonary epithelial-mesenchymal transition (EMT) and the effectiveness of Sp1 transcription factor as a target for the gene therapy on lung fibrosis.
authors.affiliation: Department of Pathology, Catholic University of Daegu, College of Medicine, 3056-6, Daemyung 4-Dong, Nam-Gu, Daegu 705-718, Republic of Korea.
authors.forename: Yoon-Seup
authors.initials: YS
authors.lastname: Kum
authors.forename: Kyung-Hyun
authors.initials: KH
authors.lastname: Kim
authors.forename: Tae-In
authors.initials: TI
authors.lastname: Park
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Suh
authors.forename: Hoon-Kyu
authors.initials: HK
authors.lastname: Oh
authors.forename: Chang-Ho
authors.initials: CH
authors.lastname: Cho
authors.forename: Jae-Bok
authors.initials: JB
authors.lastname: Park
authors.forename: Young-Chae
authors.initials: YC
authors.lastname: Chang
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Kwang-Gil
authors.initials: KG
authors.lastname: Lee
authors.forename: Kwan-Kyu
authors.initials: KK
authors.lastname: Park
mesh_terms: D000818:Animals; D018390:Gene Targeting / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009841:Oligonucleotides / Q000008:administration & dosage* / Q000235:genetics*; D011658:Pulmonary Fibrosis / Q000235:genetics / Q000378:metabolism* / Q000628:therapy*; D016329:Sp1 Transcription Factor / Q000235:genetics*; D016896:Treatment Outcome
chemical_list: D009841:Oligonucleotides; D016329:Sp1 Transcription Factor