title: Cardiovascular distribution of the calcium sensing receptor before and after burns.
abstract: Due to up-regulation of the parathyroid gland calcium-sensing receptor (CaR), burned children have hypocalcemic hypoparathyroidism, and decreased myocardial contractility. Our aim was to localize the CaR in the heart and measure receptor density changes due to burns. Heart and aorta samples from sheep subjected to 40% burn or sham conditions were probed for CaR via fluorescence microscopy. CaR was localized to endocardial endothelium, myocardial microvasculature, and fibroblasts and vessels of the aortic adventitia. CaR was not found in cardiomyocytes or smooth muscle cells. No differences in density of CaR or beta-adrenergic receptors were noted. No differences in CaR distribution were seen in the myocardium or aorta, in contrast to the parathyroid where burn injury up-regulates CaR. We suggest that CaR has a local, tissue-specific role, and functions in vascular calcium sensing for intravascular calcium deposition or regulation of other calcium channels after trauma or burn.
authors.affiliation: Department of Pediatrics, University of Texas Medical Branch Galveston, Galveston, TX, United States.
authors.forename: Gordon L
authors.initials: GL
authors.lastname: Klein
authors.forename: Perenlei
authors.initials: P
authors.lastname: Enkhbaatar
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Traber
authors.forename: L Max
authors.initials: LM
authors.lastname: Buja
authors.forename: Colette C
authors.initials: CC
authors.lastname: Jonkam
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Poindexter
authors.forename: Roger J
authors.initials: RJ
authors.lastname: Bick
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism*; D002056:Burns / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D044169:Receptors, Calcium-Sensing / Q000378:metabolism*; D012756:Sheep
chemical_list: D044169:Receptors, Calcium-Sensing