title: Over-expression of neurotensin high-affinity receptor 1 (NTS1) in relation with its ligand neurotensin (NT) and nuclear beta-catenin in inflammatory bowel disease-related oncogenesis.
abstract: We investigated the expression of the neurotensin high-affinity receptor 1 (NTS1) during inflammatory bowel disease (IBD)-related colorectal oncogenesis, in colonic samples from 30 patients with IBD-related adenocarcinomas, dysplasias, and inflammatory mucosa (IM). The percentage of NTS1-positive epithelial cells progressively increased from the inflammatory condition to adenocarcinoma and was significantly higher in adenocarcinomas than in IM (p=0.0169). In parallel, the percentage of neurotensin (NT)-positive epithelial cells increased during the IBD-related oncogenesis. Finally, as NTS1 is a ss-catenin inducible gene, we found that a number of preneoplastic lesions and adenocarcinomas co-expressed NTS1 and beta-catenin without NT expression. Therefore, this study suggests two pathways of NTS1 overexpression during IBD-related oncogenesis: one triggered by NT overexpression, and a second associated with an activation of the APC/beta-catenin pathway, these two pathways being not mutually exclusive.
authors.affiliation: INSERM, U539, Nantes, F-44035 France. u539@nantes.inserm.fr
authors.forename: Céline
authors.initials: C
authors.lastname: Bossard
authors.forename: Frédérique
authors.initials: F
authors.lastname: Souazé
authors.forename: Anne
authors.initials: A
authors.lastname: Jarry
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bezieau
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Mosnier
authors.forename: Patricia
authors.initials: P
authors.lastname: Forgez
authors.forename: Christian L
authors.initials: CL
authors.lastname: Laboisse
mesh_terms: D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000150:complications*; D006801:Humans; D007150:Immunohistochemistry; D015212:Inflammatory Bowel Diseases / Q000150:complications / Q000378:metabolism*; D008024:Ligands; D009496:Neurotensin / Q000378:metabolism*; D018028:Receptors, Neurotensin / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D008024:Ligands; D018028:Receptors, Neurotensin; D051176:beta Catenin; D009496:Neurotensin