First detection of Dobrava hantavirus from a patient with severe haemorrhagic fever with renal syndrome by SYBR Green-based real time RT-PCR.
Dobrava hantavirus (DOBV) infection was diagnosed in a previously healthy 46-y-old hunter suffering from severe haemorrhagic fever with renal syndrome (HFRS). Specific IgM antibodies against DOBV were identified by an immunofluorescence assay, while viral nucleic acid was detected by the molecular method, confirming the diagnosis. Our results reveal an existing risk of DOBV transmission to humans in Hungary.
Jakab
Ferenc
F
Department of Genetics and Molecular Biology, Institute of Biology, University of Pécs, Ifjúság ut 6, H-7624, Pécs, Hungary. jakabf@gamma.ttk.pte.hu
Sebok
Judit
J
Ferenczi
Emoke
E
Horváth
Gyozo
G
Szucs
György
G
D000914:Antibodies, Viral / Q000097:blood; D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D018778:Hantavirus Infections / Q000503:physiopathology / Q000821:virology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000503:physiopathology / Q000821:virology*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D008875:Middle Aged; D009930:Organic Chemicals; D010802:Phylogeny; D011804:Quinolines; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D000914:Antibodies, Viral; D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D007075:Immunoglobulin M; D009930:Organic Chemicals; D011804:Quinolines; D012367:RNA, Viral; C098022:SYBR Green I