Visible light induces matrix metalloproteinase-9 expression in rat eye.
Up-regulation of matrix metalloproteinase-9 (MMP-9, gelatinase B) in the nervous system has been demonstrated when excitotoxicity-induced tissue remodeling and neuronal death occurs. Induction of MMP-9 by a natural stimulus has not been observed yet. Using RT-PCR and gelatin-zymography we demonstrated MMP-9 induction at transcriptional and protein levels in different structures of the rat eye following over-stimulation with white light. MMP-9 elevation occurred in the retina without reduction in photoreceptor number or major anatomical reorganization. A transient decrease in electroretinogram b-wave indicated the functional recovery. Retrobulbar injection of a broad-spectrum MMP-inhibitor GM6001, slowed the recovery rate of b-wave amplitude. Even room-light applied to dark-adapted awake animals induced MMP-9 increase in the retina, which suggests a role for MMP-9 in physiological functional plasticity of the nervous system, such as light adaptation. This is the first demonstration of MMP-9 induction by a sensory stimulus.
Papp
Andrea M
AM
Laboratory of Proteomics, Institute of Biology, Eötvös Loránd University, Budapest, Hungary.
Nyilas
Rita
R
Szepesi
Zsuzsanna
Z
Lorincz
Magor L
ML
Takács
Eszter
E
Abrahám
István
I
Szilágyi
Nóra
N
Tóth
Júlia
J
Medveczky
Péter
P
Szilágyi
László
L
Juhász
Gábor
G
Juhász
Gábor
G
D000221:Adaptation, Ocular / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D000818:Animals; D003623:Dark Adaptation / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D004790:Enzyme Induction / Q000528:radiation effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008027:Light; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D010775:Photic Stimulation; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects*; D013312:Stress, Physiological / Q000201:enzymology* / Q000209:etiology / Q000503:physiopathology; D014785:Vision, Ocular / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects
D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D020780:Matrix Metalloproteinase 9