DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication.
DDX3, a DEAD-box RNA helicase, binds to the hepatitis C virus (HCV) core protein. However, the role(s) of DDX3 in HCV replication is still not understood. Here we demonstrate that the accumulation of both genome-length HCV RNA (HCV-O, genotype 1b) and its replicon RNA were significantly suppressed in HuH-7-derived cells expressing short hairpin RNA targeted to DDX3 by lentivirus vector transduction. As well, RNA replication of JFH1 (genotype 2a) and release of the core into the culture supernatants were suppressed in DDX3 knockdown cells after inoculation of the cell culture-generated HCVcc. Thus, DDX3 is required for HCV RNA replication.
Ariumi
Yasuo
Y
Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Okayama 700-8558, Japan.
Kuroki
Misao
M
Abe
Ken-ichi
K
Dansako
Hiromichi
H
Ikeda
Masanori
M
Wakita
Takaji
T
Kato
Nobuyuki
N
D045744:Cell Line, Tumor; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics / Q000502:physiology*; D006801:Humans; D012367:RNA, Viral / Q000378:metabolism*; D012093:Replicon; D014779:Virus Replication
D012367:RNA, Viral; C119332:DDX3X protein, human; D053487:DEAD-box RNA Helicases