title: Sequence analysis and characterization of vacuolar-type H+ -ATPase proteolipid transcript from Acanthus ebracteatus Vah1.
abstract: The vacuolar-type H+ -ATPase (V-ATPase) is a multimeric enzyme with diverse functions in plants such as nutrient transport, flowering, stress tolerance, guard cell movement and development. A partial sequence of V-ATPase proteolipid was identified among the expressed sequence tags (ESTs) generated from Acanthus ebracteatus, and selected for full-length sequencing. The 876-nucleotide cDNA consists of an open reading frame of 165 amino acids. The deduced amino acid sequence displays high similarity (81%) with its homologs from Arabidopsis thaliana, Avecinnia marina and Gossypium hirsutum with the four transmembrane domains characteristics of the 16 kDa proteolipid subunit c of V-ATPase well conserved in this protein. Southern analysis revealed the existence of several members of proteolipid subunit c of V-ATPase in A. ebracteatus. The mRNA of this gene was detected in leaf, floral, stem and root tissues, however, the expression level was lower in stem and root tissues.
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. clho@biotech.upm.edu.my
authors.forename: Chai-Ling
authors.initials: CL
authors.lastname: Ho
authors.forename: Phuoc Dang
authors.initials: PD
authors.lastname: Nguyen
authors.forename: Jennifer Ann
authors.initials: JA
authors.lastname: Harikrishna
authors.forename: Raha Abdul
authors.initials: RA
authors.lastname: Rahim
mesh_terms: D027901:Acanthaceae / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011510:Proteolipids / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D025262:Vacuolar Proton-Translocating ATPases / Q000737:chemistry / Q000235:genetics*; D014617:Vacuoles / Q000201:enzymology / Q000235:genetics
chemical_list: D010940:Plant Proteins; D011510:Proteolipids; D012333:RNA, Messenger; D025262:Vacuolar Proton-Translocating ATPases