title: A reversed-phase high-performance liquid chromatographic method for the determination of aloesin, aloeresin A and anthraquinone in Aloe ferox.
abstract: A reversed-phase HPLC method for the quantification of aloesin, aloeresin a and anthraquinone (as barbaloin) in Aloe ferox Miller and aloe-related products has been developed and validated. The method utilized a C18 column with a water-methanol gradient and UV detection at 297 nm. The method validation included linearity, accuracy, precision, limit of detection, limit of quantitation, specificity and standard solution stability. The method showed good linearity (r > 0.99 for all components) and recovery (>85% for all components). The detection and quantitation limits for barbaloin were determined to be 0.02 and 0.1 ppm at signal-to-noise ratios of approximately 3:1 and 10:1, respectively.
authors.affiliation: Unigen, 2660 Willamette Drive NE, Lacey, WA 98516, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Zahn
authors.forename: Thao
authors.initials: T
authors.lastname: Trinh
authors.forename: Mijeong Lee
authors.initials: ML
authors.lastname: Jeong
authors.forename: Desheng
authors.initials: D
authors.lastname: Wang
authors.forename: Padmapriya
authors.initials: P
authors.lastname: Abeysinghe
authors.forename: Qi
authors.initials: Q
authors.lastname: Jia
authors.forename: Wenwen
authors.initials: W
authors.lastname: Ma
mesh_terms: D000504:Aloe / Q000737:chemistry*; D000880:Anthraquinones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002867:Chromones / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D015394:Molecular Structure; D012680:Sensitivity and Specificity
chemical_list: D000880:Anthraquinones; D002867:Chromones; D005960:Glucosides; C521199:aloeresin A; C069868:aloesin