New monolithic enzymatic micro-reactor for the fast production and purification of oligogalacturonides.
Fast production and purification of alpha-(1,4)-oligogalacturonides was investigated using a new enzymatic reactor composed of a monolithic matrix. Pectin lyase from Aspergillus japonicus (Sigma) was immobilized on CIM-disk epoxy monolith. Studies were performed on free pectin lyase and immobilized pectin lyase to compare the optimum temperature, optimum pH, and thermal stability. It was determined that optimum temperature for free pectin lyase and immobilized pectin lyase on monolithic support is 30 degrees C, and optimum pH is 5. Monolithic CIM-disk chromatography is one of the fastest liquid chromatographic method used for separation and purification of biomolecules due to high mass transfer rate. In this context, online one step production and purification of oligogalacturonides was investigated associating CIM-disk pectin lyase and CIM-disk DEAE. This efficient enzymatic bioreactor production of uronic oligosaccharides from polygalacturonic acid (PGA) constitutes an original fast process to generate bioactive oligouronides.
Delattre
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Centre for BioSeparation Technology, Vellore Institute of Technology, University, Vellore 632014, Tamil Nadu, India. cedric_delattre@hotmail.com
Michaud
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Vijayalakshmi
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D019149:Bioreactors; D002846:Chromatography, Affinity / Q000379:methods*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009844:Oligosaccharides / Q000302:isolation & purification* / Q000378:metabolism; D011133:Polysaccharide-Lyases / Q000378:metabolism*; D015203:Reproducibility of Results; D013696:Temperature
D004800:Enzymes, Immobilized; D009844:Oligosaccharides; C412763:oligogalacturonic acid; D011133:Polysaccharide-Lyases; C027349:pectin lyase