title: Thrombin generation in plasma of healthy adults and children: chromogenic versus fluorogenic thrombogram analysis.
abstract: Coagulation tests and coagulation factor assays have been complemented recently with experimental tests to measure the total amount of thrombin formed. We have presently analyzed thrombin generation of healthy adult and paediatric plasma samples via a fluorogenic and a chromogenic method. The chromogenic method was performed on the fully automated Behring Coagulation System (BCS) and fluorogenic assays via Calibrated Automated Thrombography (CAT), after coagulation induction by various tissue factor (TF) concentrations. Sample distribution and variability were analyzed for the four main coagulation parameters, derived via computerized curve analysis in each method. Results for both methods were correlated. At the recommended TF concentration (300 pM), thrombin generation via BCS was less variable than via CAT (1-6 pM), but at comparable TF concentrations (1-6 pM), the CAT sensitivity was higher than that of BCS. Inhibition of intrinsic coagulation with the anti-factor VIII antibody BO2C11 revealed that the BCS detected extrinsic coagulation exclusively, at all TF concentrations tested. In contrast, at low TF concentrations (1 and 2.5 pM), via CAT, intrinsic coagulation pathway amplification was measured. At standardized TF concentrations (300 pM in BCS vs. 2.5 pM in CAT), different reference values between adults and children were found, for all parameters, except Tmax. In adult samples, the best correlation between both methods was observed for ETP(CAT) versus ETP(BCS) and for Peak height(CAT) versus Cmax(BCS), when thrombin generation was exclusively extrinsic (300 pM in BCS vs. 6 pM in CAT). In conclusion, differential thrombin generation characteristics in BCS and CAT are relevant for their clinical applicability.
authors.affiliation: Coagulation Laboratory, Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium.
authors.forename: Katrien
authors.initials: K
authors.lastname: Devreese
authors.forename: Walter
authors.initials: W
authors.lastname: Wijns
authors.forename: Isabelle
authors.initials: I
authors.lastname: Combes
authors.forename: Soetkin
authors.initials: S
authors.lastname: Van kerckhoven
authors.forename: Marc F
authors.initials: MF
authors.lastname: Hoylaerts
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000906:Antibodies; D001777:Blood Coagulation; D001780:Blood Coagulation Tests / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D002138:Calibration; D002648:Child; D002675:Child, Preschool; D002863:Chromogenic Compounds; D003374:Coumarins; D004867:Equipment Design; D005169:Factor VIII / Q000276:immunology / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D015588:Observer Variation; D012016:Reference Values; D015203:Reproducibility of Results; D012737:Sex Factors; D013917:Thrombin / Q000378:metabolism*; D013925:Thromboplastin / Q000378:metabolism; D013997:Time Factors
chemical_list: D000906:Antibodies; D002863:Chromogenic Compounds; D003374:Coumarins; D005456:Fluorescent Dyes; D005169:Factor VIII; D013925:Thromboplastin; D013917:Thrombin