[Ultrastructure of diatom Synedra acus subsp. radians as revealed by transmission electron microscopy after mild silica dissolution].
A diatom Synedra acus subsp. radians (Kütz.) Skabitsch. has been studied by transmission electron microscopy. Examination of ultrathin sections demonstrated that silica dissolution in ammonium fluoride pH 5 under mild conditions leaves the key ultrastructural elements intact. The ultrastructure and arrangement of the cell organelles was studied during ontogeny. Silicalemma-surrounded silica deposition vesicles (SDVs) with maturating daughter valves and forming girdle bands have been identified. This method of SDV visualization offers considerable advantages over the standard approach without silica dissolution.
Bedoshvili
E D
ED
Likhoshvaĭ
E V
EV
Grachev
M A
MA
D017377:Diatoms / Q000737:chemistry / Q000648:ultrastructure*; D046529:Microscopy, Electron, Transmission; D015388:Organelles / Q000000981:diagnostic imaging*; D012822:Silicon Dioxide / Q000737:chemistry; D014463:Ultrasonography
D012822:Silicon Dioxide