The effect of dendritic cells on the retinal cell transplantation.
The potential of bone marrow cell-derived immature dendritic cells (myeloid iDCs) in modulating the efficacy of retinal cell transplantation therapy was investigated. (1) In vitro, myeloid iDCs but not BMCs enhanced the survival and proliferation of embryonic retinal cells, and the expression of various neurotrophic factors by myeloid iDCs was confirmed with RT-PCR. (2) In subretinal transplantation, neonatal retinal cells co-transplanted with myeloid iDCs showed higher survival rate compared to those transplanted without myeloid iDCs. (3) CD8 T-cells reactive against donor retinal cells were significantly increased in the mice with transplantation of retinal cells alone. These results suggested the beneficial effects of the use of myeloid iDCs in retinal cell transplantation therapy.
Oishi
Akio
A
Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Japan.
Nagai
Takayuki
T
Mandai
Michiko
M
Takahashi
Masayo
M
Yoshimura
Nagahisa
N
D000818:Animals; D002450:Cell Communication / Q000276:immunology*; D002470:Cell Survival / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008822:Mice, Transgenic; D012160:Retina / Q000166:cytology / Q000276:immunology* / Q000637:transplantation*