Microarray analyses of differentially expressed human genes and biological processes in ECV304 cells infected with rubella virus.
Changes in mRNA expression levels of ECV304 cells infected with the wild-type rubella strain were analyzed using a microarray system representing 18,716 human genes. Four hundred eighty-seven genes exhibited differential expression levels; 456 of these genes were up-regulated while 31 genes were down-regulated. We identified 53 biological processes that were significantly relevant to the RV-infection. Among these biological processes, 52 were one-gene processes and one was a process involving five genes: IFNA21 (interferon, alpha 21), interferon stimulated exonuclease gene 20 kDa (ISG20), zinc finger protein 175 (ZNF175), tripartite motif-containing 22 (TRIM22), and MX2 [myxovirus (influenza virus) resistance 2 (mouse)]. Except for ZNF175, gene annotation indicated four of these genes encoded interferon or interferon-induced genes. These results suggest that genes relevant to interferon-regulated pathways may be involved in the pathogenesis of rubella.
Mo
Xiao-yang
XY
Center for Heart Development, Key Lab of National Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan, China. moxiaoyang@yahoo.com.cn
Ma
Wenli
W
Zhang
Yali
Y
Zhao
Haiquan
H
Deng
Yun
Y
Yuan
Wuzhou
W
Wang
Yuequn
Y
Li
Yongqin
Y
Zhu
Chuanbing
C
Liu
Mingyao
M
Wu
Xiushan
X
D002460:Cell Line; D042783:Endothelial Cells / Q000821:virology*; D004730:Endothelium, Vascular / Q000166:cytology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012412:Rubella virus / Q000472:pathogenicity*; D014471:Umbilical Veins
D011506:Proteins