A mev-1-like dominant-negative SdhC increases oxidative stress and reduces lifespan in Drosophila.
Oxidative stress is thought to promote aging by damaging various cellular components. We investigated the phenotypic effects of mutation in the sdhC gene encoding succinate dehydrogenase C in Drosophila by using transgenic flies expressing a dominant-negative form, SdhC(I71E). Expression of SdhC(I71E) significantly reduced the mean lifespan by 22% compared to that of control flies. The amount of protein carbonyl was significantly increased, suggesting that a high level of oxidative stress was induced in these flies. The transgenic flies should serve as a model for studying the biological processes associated with increased levels of oxidative stress and mitochondrial dysfunction in Drosophila.
Tsuda
Manabu
M
Department of Biological Sciences, Tokyo Metropolitan University, 1-1 Minami-osawa, Hachioji-shi, Tokyo 192-0397, Japan.
Sugiura
Tetsuro
T
Ishii
Takamasa
T
Ishii
Naoaki
N
Aigaki
Toshiro
T
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D045303:Cytochromes b; D004330:Drosophila / Q000502:physiology*; D008136:Longevity / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology*; D013385:Succinate Dehydrogenase / Q000378:metabolism*
D001426:Bacterial Proteins; D029742:Caenorhabditis elegans Proteins; C472666:MEV-1 protein, C elegans; C486355:SdhC protein, bacteria; D045303:Cytochromes b; D013385:Succinate Dehydrogenase