Identification of a gene encoding the light-harvesting chlorophyll a/b proteins of photosystem I in green alga Dunaliella salina.
There are four LhcII genes of Dunaliella salina have been submitted to the database of GenBank. However, little is known about Lhca genes of this green alga, although this knowledge might be available to study the composition and phylogenesis of Lhc gene family. Recently, one Lhca gene was been cloned from the green alga D. salina by PCR amplification using degenerate primers. This cDNA, designated as DsLhca1, contains an open reading frame encoded a protein of 222 amino acids with a calculated molecular mass of 27.8 kDa. DsLhca1 is predicted to contain three transmembrane domains and a N-terminal chloroplast transit peptide (cTP) with length of 33 amino acids. The genomic sequence of DsLhca1 is composed of five introns. The deduced polypeptide sequence of this gene showed a lower degree of identity (less than 30%) with LHCII proteins from D. salina. But its homology to Lhca proteins of other algae (Volvox carteri Lhca_AF110786) was higher with pairwise identities of up to 67.1%. Phylogenetic analysis indicated that DsLhcal protein cannot be assigned to any types of Lhca proteins in higher plants or in Chlamydomonas reinhardtii.
Liang
Xue
X
Key Laboratory of Bio-resources and Eco-environment, Ministry of Education (Sichuan University), Sichuan Public Experimental Platform of Bioinformatics and Metabolic Engineering, Sichuan 610064, P R. China. xue_liang227@hotmail.com
Qiao
Dairong
D
Huang
Min
M
Yi
Xiuli
X
Bai
Linhan
L
Xu
Hui
H
Wei
Liang
L
Zeng
Jing
J
Cao
Yi
Y
D020418:Algal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D000460:Chlorophyta / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D045331:Photosystem I Protein Complex / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny
D020418:Algal Proteins; D045342:Light-Harvesting Protein Complexes; D045331:Photosystem I Protein Complex; D002734:Chlorophyll; C037184:chlorophyll b; D000077194:Chlorophyll A