Screening for the anti-inflammatory activity of fractions and compounds from Atractylodes macrocephala koidz.
The aim of this study was to screen for the anti-inflammatory activity of fractions and compounds from Atractylodes macrocephala Koidz. The rhizomes of Atractylodes macrocephala were treated with supercritical CO(2) fluid and the extract was separated by normal-phase and reverse-phase column chromatography. The separated samples were screened with white blood cell membrane (WBCM) chromatography (WBCM-C). The anti-inflammatory effects of these fractions and components were tested pharmacologically in vivo. The results indicated that the retention characteristics of the petrol-ether (1:1, v/v) fraction (BZC-2) of the supercritical CO(2) extract, the atractylenolide I and 14-acetoxy-12-senecioyloxytetradeca-2E,8E,10E-trien-4, 6-diyn-1-ol isolated from BZC-2 as active fractions and components were similar to that of dexamethasone in WBCM-C. Therefore, they may act on WBCM and its receptors. BZC-2 has shown anti-inflammatory effects in acute and chronic inflammation models in rats and mice. Oral administration of atractylenolide I and 14-acetoxy-12-senecioyloxytetradeca-2E,8E,10E-trien-4,6-diyn-1-ol produced significant anti-inflammatory effects in acute and chronic inflammation models in mice. The screening results with WBCM-C were correlated significantly with pharmacological effects in vivo. Atractylenolide I and 14-acetoxy-12-senecioyloxytetradeca-2E,8E,10E-trien-4,6-diyn-1-ol were the main components of Atractylodes macrocephala that were effective as anti-inflammatory agents.
Li
Cui-Qin
CQ
School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
He
Lang-Chong
LC
Dong
Hai-Yan
HY
Jin
Ju-Qing
JQ
D019342:Acetic Acid / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D031174:Atractylodes / Q000737:chemistry*; D002199:Capillary Permeability / Q000187:drug effects; D002351:Carrageenan; D002462:Cell Membrane / Q000187:drug effects; D002845:Chromatography; D032561:Cotton Fiber; D003907:Dexamethasone / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D005260:Female; D006099:Granuloma / Q000473:pathology / Q000517:prevention & control; D007962:Leukocytes / Q000737:chemistry / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D011817:Rabbits
D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D010936:Plant Extracts; D003907:Dexamethasone; D002351:Carrageenan; D019342:Acetic Acid