Emergence of the quinolone resistance-mediating gene aac(6')-Ib-cr in extended-spectrum-beta-lactamase-producing Klebsiella isolates collected in Slovenia between 2000 and 2005.
Seventy-four nonrepetitive uropathogenic fluoroquinolone-resistant or -intermediate extended-spectrum-beta-lactamase-producing Klebsiella isolates from Slovenia were screened for the presence of plasmid-mediated quinolone resistance genes. None of the known qnr genes were detected. The aac(6')-Ib-cr allele was detected on plasmids from 25 transconjugants for which the ciprofloxacin MIC was higher than for the recipient Escherichia coli strain.
Ambrozic Avgustin
Jerneja
J
Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, 1000 Ljubljana, Slovenia. Jerneja.Ambrozic@bf.uni-lj.si
Keber
Rok
R
Zerjavic
Katja
K
Orazem
Toni
T
Grabnar
Miklavz
M
D002939:Ciprofloxacin / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004756:Enterobacteriaceae Infections / Q000382:microbiology / Q000652:urine; D006801:Humans; D007709:Klebsiella / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D015363:Quinolones / Q000494:pharmacology*; D017524:Slovenia; D001618:beta-Lactamases / Q000378:metabolism*
D004269:DNA, Bacterial; D015363:Quinolones; D002939:Ciprofloxacin; D001618:beta-Lactamases