Nutritional quality evaluation of velvet bean seeds (Mucuna pruriens) exposed to gamma irradiation.
Effects of gamma irradiation on Mucuna pruriens seeds at various doses (0, 2.5, 5, 7.5, 10, 15 and 30 kGy) on the proximate composition, mineral constituents, amino acids, fatty acids and functional properties were investigated. Gamma irradiation resulted in a significant increase of crude protein at all doses, while the crude lipid, crude fibre and ash showed a dose-dependent decrease. Raw Mucuna seeds were rich in minerals (potassium, phosphorus, calcium, magnesium, iron and selenium). Sodium, copper and manganese were significantly decreased on irradiation at all the doses, while magnesium and iron showed a significant decrease only above 10 kGy. The essential amino acids of raw and gamma-irradiated Mucuna seeds were comparable with the FAO/WHO recommended pattern. A significant increase of in vitro protein digestibility was seen in seeds irradiated at 30 kGy. High amounts of unsaturated fatty acids in Mucuna seeds decreased significantly after irradiation. However, linoleic acid was not present in raw seeds but detected after irradiation and it was elevated to high level at 30 kGy. Behenic acid, a major anti-nutritional factor, was reduced significantly on irradiation, indicating the positive effect of gamma irradiation on Mucuna seeds. Significant enhancement in the water absorption and oil absorption capacities, protein solubility, emulsion activity and improvement in the gelation capacity was recorded after irradiation. Results of the present investigation reveal that application of gamma irradiation does not affect the overall nutritional composition and can be used as an effective method of preservation of Mucuna seed and their products.
Bhat
Rajeev
R
Microbiology and Biotechnology, Department of Biosciences, Mangalore University, Mangalagangotri, Mangalore, Karnataka, India.
Sridhar
Kandikere R
KR
Seena
Sahadevan
S
D000596:Amino Acids / Q000032:analysis; D004063:Digestion; D004307:Dose-Response Relationship, Radiation; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D005514:Food Irradiation; D005720:Gamma Rays; D008055:Lipids / Q000032:analysis; D031299:Mucuna / Q000737:chemistry* / Q000528:radiation effects*; D009753:Nutritive Value; D014674:Plant Proteins, Dietary / Q000032:analysis / Q000378:metabolism; D011829:Radiation Dosage; D012639:Seeds / Q000737:chemistry / Q000528:radiation effects; D012995:Solubility; D014131:Trace Elements / Q000032:analysis
D000596:Amino Acids; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D014674:Plant Proteins, Dietary; D014131:Trace Elements