Speed-mapping quantitative trait loci using microarrays.
We developed a rapid, economical method for high-resolution quantitative trait locus (QTL) mapping using microarrays for selective genotyping of pooled DNA samples. We generated 21,207 F2 flies from two inbred Drosophila melanogaster strains with known QTLs affecting lifespan, and hybridized DNA pools of young and old flies to microarrays. We used changes of gene frequency of 2,326 single-feature polymorphisms (SFPs) to map previously identified and additional QTLs affecting lifespan.
Lai
Chao-Qiang
CQ
Jean Mayer-US Department of Agriculture Human Nutrition Research Center on Aging, Nutrition and Genomics, Tufts University, 711 Washington Street, Boston, Massachusetts 02111, USA. chaoqiang.lai@ars.usda.gov
Leips
Jeff
J
Zou
Wei
W
Roberts
Jessica F
JF
Wollenberg
Kurt R
KR
Parnell
Laurence D
LD
Zeng
Zhao-Bang
ZB
Ordovas
Jose M
JM
Mackay
Trudy F C
TF
D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D008136:Longevity / Q000235:genetics; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011110:Polymorphism, Genetic / Q000235:genetics; D040641:Quantitative Trait Loci