Immunological roles of Pasteurella multocida toxin (PMT) using a PMT mutant strain.
The immunological role of the Pasteurella multocida toxin (PMT) in mice was examined using a PMT mutant strain. After a nasal inoculation, the mutant strain failed to induce interstitial pneumonia. Moreover, PMT had no significant effect on the populations of CD4+, CD8+, CD3+, and CD19+ immunocytes in blood or on the populations of CD4+ and CD8+ splenocytes (P<0.01). However, there was a significant increase in the total number of cells in the BAL samples obtained from the wild-type P. multocida-inoculated mice. On the other hand, the level of IL-1 expression decreased when the macrophages from the bronchio-alveolar lavage were stimulated with PMT. Overall, PMT appears to play some role (stimulating and/or inhibiting) in the immunological responses but further studies will be required to confirm this.
Kim
Tae Jung
TJ
Biotheraphy Human Resources Center, Chonnam National University, Gwangju 500-757, Republic of Korea.
Toan
Nguyen Tat
NT
Jang
Eun Jin
EJ
Jung
Bock Gie
BG
Lee
Jae Il
JI
Lee
Bong Joo
BJ
D000818:Animals; D018941:Antigens, CD19 / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D017252:CD3 Complex / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002452:Cell Count; D053583:Interleukin-1beta / Q000378:metabolism; D008168:Lung / Q000276:immunology / Q000382:microbiology; D017563:Lung Diseases, Interstitial / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D010326:Pasteurella Infections / Q000276:immunology* / Q000382:microbiology; D016979:Pasteurella multocida / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
D018941:Antigens, CD19; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D017252:CD3 Complex; D053583:Interleukin-1beta; C062258:Pasteurella multocida toxin