Resveratrol inhibits the activity of equine neutrophil myeloperoxidase by a direct interaction with the enzyme.
Resveratrol is a polyphenolic antioxidant present in beverage and food known for its multiple protective effects. We report the inhibitory effects of resveratrol on equine myeloperoxidase (MPO), a hemic peroxidase present in the granules of the neutrophils involved in the inflammatory response. Resveratrol inhibited the production of reactive oxygen species (ROS) by stimulated equine neutrophils by acting as a direct scavenger of the ROS released by the cells but did not modify the degranulation of the stimulated neutrophils as the amounts of released MPO were unchanged. Resveratrol strongly inhibited the chlorination, oxidation, and nitration activities of MPO in a dose-dependent manner. By an original technique of specific immunological extraction followed by enzymatic detection (SIEFED), we demonstrated that resveratrol inhibited the peroxidasic activity of the MPO measured by a direct interaction such as the fixation of resveratrol on the enzyme. The observation of a decrease of the accumulation of compound II suggested that resveratrol acts as an electron donor for MPO reduction.
Kohnen
Stephan
S
Department of Clinical Sciences, Large Animal Surgery, Faculty of Veterinary Medicine B41, University of Liège, Sart Tilman, 4000 Liège, Belgium. S.Kohnen@ulg.ac.be
Franck
Thierry
T
Van Antwerpen
Pierre
P
Boudjeltia
Karim Zouaoui
KZ
Mouithys-Mickalad
Ange
A
Deby
Carol
C
Moguilevsky
Nicole
N
Deby-Dupont
Ginette
G
Lamy
Maurice
M
Serteyn
Didier
D
D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006736:Horses; D009504:Neutrophils / Q000201:enzymology*; D009195:Peroxidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D017382:Reactive Oxygen Species; D013267:Stilbenes; D009195:Peroxidase; D000077185:Resveratrol