title: [Mechanism of salvianolate in preventing postoperative intestinal adhesion in rats].
abstract: OBJECTIVE
  To observe the effects of salvianolate on rats with postoperative intestinal adhesion and to explore the prevention mechanism.
  
  
  METHODS
  Forty SD male rats with intestinal adhesion were randomly divided into four groups: untreated group, low-dose salvianolate-treated group (12 mg/kg), medium-dose salvianolate-treated group (24 mg/kg) and high-dose salvianolate-treated group (48 mg/kg), with another ten SD male rats as normal control. Intraperitoneal injection of glucose was administered to the rats in the normal control group and the untreated group, and intraperitoneal injection of salvianolate was administered to the rats in the low-, medium- and high-dose salvianolate-treated groups. They were all treated for 8 days and once a day. On the eighth day after surgery the blood samples of each group were collected. Grades of intestinal adhesion were ranked by macroscopic observation. The adhesive tissues between viscera and belly wall were taken for pathological observation. The levels of interleukin-1beta (IL-1beta), interleukin-4 (IL-4) and tumor necrosis factor-alpha (TNF-alpha) were determined by enzyme linked immunosorbent assay.
  
  
  RESULTS
  Salvianolate can significantly reduce the extent of postoperative intestinal adhesion, obviously decrease the levels of IL-1beta, TNF-alpha and inhibit the hyperplasy of fibrous connective tissue. However, there was no significant impact on the level of IL-4.
  
  
  CONCLUSIONS
  Salvianolate can reduce the extent of postoperative intestinal adhesion, decrease the expression of IL-1beta and TNF-alpha and inhibit the hyperplasy of fibrous connective tissue. This may be the mechanism of salvianolate in preventing intestinal adhesion.
authors.affiliation: First Clinical Medical College, Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang Province 310053, China.
authors.forename: Xin-bing
authors.initials: XB
authors.lastname: Sui
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Hua-sen
authors.initials: HS
authors.lastname: Qiu
authors.forename: Ji-chun
authors.initials: JC
authors.lastname: Zhou
authors.forename: Xi-dong
authors.initials: XD
authors.lastname: Gu
authors.forename: Zhou-xiang
authors.initials: ZX
authors.lastname: Lu
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Jin
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
mesh_terms: D000005:Abdomen / Q000601:surgery*; D000818:Animals; D002109:Caffeic Acids / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000378:metabolism; D007410:Intestinal Diseases / Q000378:metabolism / Q000517:prevention & control*; D007773:Lactates / Q000494:pharmacology*; D008297:Male; D011183:Postoperative Complications / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry; D000267:Tissue Adhesions / Q000378:metabolism / Q000517:prevention & control; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D002109:Caffeic Acids; D053583:Interleukin-1beta; D007773:Lactates; D014409:Tumor Necrosis Factor-alpha; C066201:salvianolic acid A