title: MegaPlex PCR: a strategy for multiplex amplification.
abstract: 'MegaPlex PCR' is a robust technology for highly multiplexed amplification of specific DNA sequences. It uses target-specific pairs of PCR primers that are physically separated by surface immobilization. Initial surface-based amplification cycles are then coupled to efficient solution-phase PCR using one common primer pair. We demonstrate this method by co-amplifying and genotyping 75 unselected human single-nucleotide polymorphism (SNP) loci.
authors.affiliation: Department of Genetics, University of Leicester, University Road, Leicester, LE1 7RH, UK.
authors.forename: Linda Strömqvist
authors.initials: LS
authors.lastname: Meuzelaar
authors.forename: Owen
authors.initials: O
authors.lastname: Lancaster
authors.forename: J Paul
authors.initials: JP
authors.lastname: Pasche
authors.forename: Guido
authors.initials: G
authors.lastname: Kopal
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Brookes
mesh_terms: D017931:DNA Primers; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers