Kinetic and functional analysis of the heparin-binding domain of fibronectin.
We have previously shown that the heparin-binding domain of fibronectin (FN-HBD) enhances cell adhesion and proliferation of osteoblasts. Here we demonstrated that FN-HBD binds to heparin with a K(D) of 5 microM. Although, FN-HBD itself produces a modest effect on cell adhesion in the absence of central cell-binding domain (CCBD), FN-HBD significantly enhances cell adhesion and spreading activities by a cooperative mechanism of CCBD in MG63 cells (P < 0.05).
Kang
Wonmo
W
Department of Biochemistry, Inha University College of Medicine, Incheon 400-712, Korea.
Park
Sunok
S
Jang
Jun-Hyeog
JH
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D005353:Fibronectins / Q000737:chemistry* / Q000378:metabolism*; D006493:Heparin / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008657:Metabolic Clearance Rate; D012516:Osteosarcoma / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary
D005353:Fibronectins; D006493:Heparin