title: RCAS1 decidual immunoreactivity in severe pre-eclampsia: immune cell presence and activity.
abstract: OBJECTIVE
  Pre-eclampsia seems to be related to the disturbance of immune tolerance regulation during pregnancy. Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) decidual level alterations were concomitant with changes in immune cell number and activity in decidua. As decidual immunomodulating activity participates in the development of immune tolerance during pregnancy, we aimed to evaluate the immunoreactivity level of decidual RCAS1 with respect to the presence and activity of immune cells.
  
  
  METHODS
  RCAS1, CD3, CD56, CD69, and CD25 immunoreactivity was assessed by immunohistochemistry in 30 decidual samples derived from patients with severe pre-eclampsia (sPE) and from a healthy control group.
  
  
  RESULTS
  RCAS1 immunoreactivity was statistically significantly higher in decidual tissue samples derived from patients with sPE tissue than in those derived from healthy patients in whom elective cesarean section at term was performed. A statistically significantly lower number of CD56(+) and CD3(+) cells and lower immunoreactivity level of CD69 were found in patients with sPE compared with those from the control group.
  
  
  CONCLUSIONS
  The limited immune cells infiltration in decidua during sPE is associated with increase in RCAS1 decidual level.
authors.affiliation: Department of Gynecology and Oncology of the Jagiellonian University, Krakow, Poland. owicher@cyf-kr.edu.pl
authors.forename: Lukasz
authors.initials: L
authors.lastname: Wicherek
authors.forename: Pawel
authors.initials: P
authors.lastname: Basta
authors.forename: Jerzy
authors.initials: J
authors.lastname: Sikora
authors.forename: Krystyna
authors.initials: K
authors.lastname: Galazka
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Rytlewski
authors.forename: Marek
authors.initials: M
authors.lastname: Grabiec
authors.forename: Agata
authors.initials: A
authors.lastname: Lazar
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Kalinka
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis*; D017252:CD3 Complex / Q000032:analysis; D019002:CD56 Antigen / Q000032:analysis; D016022:Case-Control Studies; D003656:Decidua / Q000276:immunology*; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D007150:Immunohistochemistry; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis; D037181:Lectins, C-Type; D007958:Leukocyte Count; D011225:Pre-Eclampsia / Q000097:blood / Q000276:immunology*; D011247:Pregnancy; D012720:Severity of Illness Index; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D017252:CD3 Complex; D019002:CD56 Antigen; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D000099192:CD69 Antigens; C120762:EBAG9 protein, human