The use of biotin-avidin binding to facilitate biomodification of thermoresponsive culture surfaces.
Here, we report biomodification of temperature-responsive culture surfaces with biotinylated biomolecules utilizing streptavidin and biotinylation of the surfaces. Poly(N-isopropylacrylamide-co-2-carboxyisopropylacrylamide) was covalently grafted onto tissue culture polystyrene (TCPS) dishes. Biotinylated Arg-Gly-Asp-Ser (RGDS) peptides with different spacer lengths (biotin-conjugated G(n)RGDS (n=1,6,12,16)) were examined. Human umbilical vein endothelial cells (HUVECs) adhered and were well spread on G(12)RGDS-immobilized surfaces in the absence of serum at 37 degrees C, while much less cell adhesion was observed with the other peptides. Adhered HUVECs were detached on reducing temperature to 20 degrees C, or on adding free RGDS peptide. Interestingly, cell detachment was accelerated by applying both these techniques. Consequently, by optimizing the spacer length, biomolecules can be functionally immobilized onto thermoresponsive surfaces via the affinity binding between avidin and biotin.
Nishi
Masanori
M
Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, Japan.
Kobayashi
Jun
J
Pechmann
Sebastian
S
Yamato
Masayuki
M
Akiyama
Yoshikatsu
Y
Kikuchi
Akihiko
A
Uchida
Katsumi
K
Textor
Marcus
M
Yajima
Hirofumi
H
Okano
Teruo
T
D001360:Avidin / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D006801:Humans; D009842:Oligopeptides / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature
D009842:Oligopeptides; D011108:Polymers; D001360:Avidin; D001710:Biotin; C046617:arginyl-glycyl-aspartyl-serine