Comparison of the effects of desflurane and sevoflurane on the expression of platelet surface glycoproteins in unstimulated and adenosine diphosphate-induced platelets in vitro.
OBJECTIVE
To compare the effects of one minimum alveolar concentration (MAC) desflurane and sevoflurane on the expression of CD42b (glycoprotein [GP] Ib), CD41 (GPIIb), CD61 (GPIIIa), CD62P (P-selectin), and CD63 in both unstimulated and adenosine diphosphate (ADP)-stimulated platelets in vitro.


METHODS
University laboratory.


METHODS
15 healthy volunteers.


METHODS
Platelet-rich plasma was obtained and divided into three groups: platelet-rich plasma exposed to air (group 1); air plus one MAC desflurane (6% vol; group 2), and air plus one MAC sevoflurane (2% vol; group 3), for 40 minutes. Percentage of antigen-positive cells (%(+)) mean channel fluorescence (MCF(Sigma)), and index of platelet activation for positive platelets (IPA(+)) as expression markers for GPIb, GPIIb, GPIIIa, P-selectin, and CD63, were measured.


RESULTS
In unstimulated platelets, expression markers for GPIIb and GPIIIa were significantly lower in groups 2 and 3 than group 1 (P < 0.001). P-selectin expression markers were significantly higher in group 2 than in group 1 or group 3 (P < 0.016). CD63 expression markers were significantly lower in group 3 than group 1 (P < 0.016). In ADP-stimulated platelets, expression markers for all glycoproteins were significantly higher in all groups.


CONCLUSIONS
Neither one MAC desflurane nor sevoflurane showed any significant change in ADP-stimulated platelets compared with the control group.
Koroglu
Ahmet
A
Department of Anesthesiology and Reanimation, Inonu University Faculty of Medicine, Malatya 44315, Turkey. akoroglu@inonu.edu.tr
Cicek
Muslum
M
Toprak
Huseyin I
HI
Karakoc
Yunus
Y
Noyan
Funda
F
Ersoy
Ozcan M
OM
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D000077335:Desflurane; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007530:Isoflurane / Q000031:analogs & derivatives* / Q000494:pharmacology; D008738:Methyl Ethers / Q000494:pharmacology*; D019007:P-Selectin / Q000097:blood; D015539:Platelet Activation / Q000187:drug effects; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D011446:Prospective Studies; D000077149:Sevoflurane
D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D000077149:Sevoflurane; D000244:Adenosine Diphosphate; D000077335:Desflurane; D007530:Isoflurane