title: Floxed allele for conditional inactivation of the voltage-gated sodium channel beta1 subunit Scn1b.
abstract: The voltage-gated sodium channel gene Scn1b encodes the auxiliary subunit beta1, which is widely distributed in neurons and glia of the central and peripheral nervous systems, cardiac myocytes, skeletal muscle myocytes, and neuroendocrine cells. We showed previously that the Scn1b null mutation results in a complex and severe phenotype that includes retarded growth, seizures, ataxia, and death by postnatal day 21. We generated a floxed allele of Scn1b by inserting loxP sites surrounding the second coding exon. Ubiquitous deletion of the floxed exon by Cre recombinase using CMV-Cre-transgenic mice produced the Scn1b(del) allele. The null phenotype of Scn1b(del) homozygotes is indistinguishable from that of Scn1b nulls and confirms the invivo inactivation of Scn1b. Conditional inactivation ofthe floxed allele will make it possible to circumvent the lethality that results from complete loss of this gene, such that the physiological role of Scn1b in specific cell types and/or specific developmental time points can be investigated.
authors.affiliation: Department of Pharmacology, University of Michigan, Ann Arbor, Michigan 48109-0632, USA.
authors.forename: Chunling
authors.initials: C
authors.lastname: Chen
authors.forename: Travis L
authors.initials: TL
authors.lastname: Dickendesher
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Oyama
authors.forename: Haruko
authors.initials: H
authors.lastname: Miyazaki
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Nukina
authors.forename: Lori L
authors.initials: LL
authors.lastname: Isom
mesh_terms: D000483:Alleles; D000818:Animals; D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D003433:Crosses, Genetic; D005786:Gene Expression Regulation; D018390:Gene Targeting / Q000379:methods*; D019426:Integrases / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009928:Organ Specificity; D015222:Sodium Channels / Q000235:genetics* / Q000378:metabolism; D062547:Voltage-Gated Sodium Channel beta-1 Subunit
chemical_list: C524974:Scn1b protein, mouse; D015222:Sodium Channels; D062547:Voltage-Gated Sodium Channel beta-1 Subunit; C045073:Cre recombinase; D019426:Integrases