title: Determination of camptothecins in DMSO extracts of Nothapodytes foetida by direct injection capillary electrophoresis.
abstract: A rapid capillary electrophoresis procedure was developed for determining the anti-cancer components, camptothecins, in Nothapodytes foetida. The hydrophobic compound was extracted from plant tissue (ca. 1 mL of DMSO for 100 mg of dried plant tissue) with a water-miscible organic solvent, DMSO, at elevated temperature (60 degrees C). The extract was directly injected into the separation capillary (untreated fused silica, 34 cm in length, 75 microm i.d.) and analysed in MEKC mode (369 nm). Within 5 min of migration, camptothecins were successfully separated and quantified by adding organic modifiers to the running buffer (20% DMSO, 90 mm SDS in 10 mm borate buffer, pH 8.60). The linear dynamic range for camptothecin was from 5 to 400 microg/mL. This method was proven to be very suitable for monitoring the amount of camptothecins during the cultivation of the medicinal plant.
authors.affiliation: Department of Bio-Industrial Mechatronics Engineering, College of Bioresources and Agriculture, National Taiwan University, Taipei, Taiwan.
authors.forename: Hsien-Yi
authors.initials: HY
authors.lastname: Hsiao
authors.forename: Tzong-Jih
authors.initials: TJ
authors.lastname: Cheng
authors.forename: Ge-Ming
authors.initials: GM
authors.lastname: Yang
authors.forename: I-Jen
authors.initials: IJ
authors.lastname: Huang
authors.forename: Richie L C
authors.initials: RL
authors.lastname: Chen
mesh_terms: D002166:Camptothecin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D019684:Magnoliopsida / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010936:Plant Extracts; D002166:Camptothecin; D000432:Methanol; D004121:Dimethyl Sulfoxide