Cloning and characterization of a novel human SPRYD4 gene encoding a putative SPRY domain-containing protein.
We report here the cloning and characterization of a novel human SPRYD4 gene which encodes a SPRY domain containing protein. The SPRYD4 gene is isolated from the human brain cDNA library, and mapped to 12q13.2 by searching the UCSC genomic database. The SPRYD4 cDNA is 1201 base pairs in length and contains an open reading frame encoding 207 amino acids. The SPRYD4 gene consists of two exons and encodes a putative protein with a SPRY domain ranging from 86 to 203 amino acids. The RT-PCR analysis reveals that SPRYD4 is ubiquitously expressed in 18 human tissues. However, it is strongly expressed in kidney, bladder, brain, thymus and stomach, while weakly expressed liver, testis, uterus, spleen and lung. Subcellular localization demonstrates that SPRYD4 protein is localized in the nuclear when overexpressed in COS-7 cell.
Zhong
Zhaomin
Z
State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, People's Republic of China.
Zhang
Haoxing
H
Bai
Meirong
M
Ni
Jun
J
Wan
Bo
B
Chen
Xinya
X
Yu
Long
L
D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002417:Cattle; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004285:Dogs; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary / Q000235:genetics*; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D013347:Subcellular Fractions
D009687:Nuclear Proteins; C531387:SPRYD4 protein, human