Detergent resistance as a tool in membrane research.
The biological membrane is a complicated matrix wherein different lipid environments are thought to exist. The more ordered or raft environment has been perceived biochemically accessible via its relative resistance to detergent. This paper outlines the protocols developed in our laboratory for the analysis of such detergent-resistant membranes (DRMs). We stress the fact that DRMs are artifactual in nature and should not be equivocated to lipid rafts, their usefulness being limited to assigning raft-association potential most convincingly when changes in DRM composition are induced by biochemically/physiologically relevant events. These protocols are completed in 1-2 d.
Lingwood
Daniel
D
Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany.
Simons
Kai
K
D000818:Animals; D018929:Cell Culture Techniques; D002458:Cell Fractionation / Q000379:methods*; D003902:Detergents / Q000737:chemistry*; D021962:Membrane Microdomains / Q000737:chemistry*; D008823:Micelles; D012995:Solubility
D003902:Detergents; D008823:Micelles