title: Quantitative and qualitative in vitro analysis of the stem cell potential of hematopoietic cells purified from murine skeletal muscle.
abstract: The murine skeletal muscle contains hematopoietic stem cells, but this potential has so far not been studied quantitatively or qualitatively in vitro. To quantify the hematopoietic stem cell potential, we have used highly purified SP/CD45(+) cells in long-term culture initiating cell (LTC-IC) assays. The SP/CD45(+) cell population purified from murine muscle was found to have significant stem cell activity with an LTC-IC frequency of 1/640. Single-cell-sorted SP/CD45(+) cells from muscle exhibited robust proliferative activity in vitro at day 16 (380-fold amplification), especially after culture with OP-9 layers that also support embryonic stem cells. Amplified cell populations originating from single cells exhibited multilineage differentiation ability with evidence of myeloid, lymphoid and NK cell markers. Thus, our results demonstrate that hematopoietic stem cells that can be quantified by LTC-IC assays exist in the murine skeletal muscle and show also for the first time, at the single-cell level, that these cells exhibit multilineage differentiation ability and major proliferative potential.
authors.affiliation: Inserm U362 and Translational Research Laboratory, Institut Gustave Roussy, Villejuif, France.
authors.forename: Celine
authors.initials: C
authors.lastname: Haond
authors.forename: Françoise
authors.initials: F
authors.lastname: Farace
authors.forename: Martine
authors.initials: M
authors.lastname: Guillier
authors.forename: Yann
authors.initials: Y
authors.lastname: Lécluse
authors.forename: Frederic
authors.initials: F
authors.lastname: Mazurier
authors.forename: William
authors.initials: W
authors.lastname: Vainchenker
authors.forename: Ali G
authors.initials: AG
authors.lastname: Turhan
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000166:cytology*; D010641:Phenotype
chemical_list: D017493:Leukocyte Common Antigens