title: Structural analysis of linear hydroxyproline-bound O-glycans of Chlamydomonas reinhardtii--conservation of the inner core in Chlamydomonas and land plants.
abstract: Linear hydroxyproline-bound O-glycans of the unicellular green alga Chlamydomonas reinhardtii were isolated from outer cell wall glycoproteins and their structure elucidated by chemical and spectroscopic methods. They consist exclusively of arabinose and galactose, the latter in the furanose form, unusual for plants. The first two arabinoses linked to hydroxyproline have the same anomeric configuration and linkage to each other as do the arabinosides isolated from land plants, suggesting that these two steps of hydroxyproline-O-glycosylation have been conserved during evolution. In a concomitant analysis of Hyp-bound O-tri- and tetraarabinans isolated from Arabidopsis thaliana, we were able to unambiguously identify the natural trans-4-l-Hyp-bound O-glycans and the artificially produced d-allo forms and to identify diagnostic signals in NMR spectra of these compounds for the first time.
authors.affiliation: Institut fuer Biochemie, Universitaet zu Koeln, Zuelpicher Str., 47, 50674 Koeln, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Bollig
authors.forename: Marc
authors.initials: M
authors.lastname: Lamshöft
authors.forename: Kristian
authors.initials: K
authors.lastname: Schweimer
authors.forename: Franz-Josef
authors.initials: FJ
authors.lastname: Marner
authors.forename: Herbert
authors.initials: H
authors.lastname: Budzikiewicz
authors.forename: Sabine
authors.initials: S
authors.lastname: Waffenschmidt
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000378:metabolism; D001089:Arabinose / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D016825:Chlamydomonas reinhardtii / Q000378:metabolism*; D005663:Furans / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006023:Glycoproteins / Q000737:chemistry; D006909:Hydroxyproline / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013053:Spectrophotometry / Q000379:methods
chemical_list: D005663:Furans; D006023:Glycoproteins; D011134:Polysaccharides; D001089:Arabinose; D006909:Hydroxyproline; D005690:Galactose