Abeta(1-42) stimulated T cells express P-PKC-delta and P-PKC-zeta in Alzheimer disease.
The protein kinase C (PKC) family of enzymes is a regulator of transmembrane signal transduction, and involvement of some PKC isoforms in T-cell activation has been demonstrated. Nevertheless, very little is known about their involvement in the Amyloid beta (Abeta)-dependent molecular signals in the T lymphocytes of Alzheimer disease (AD) patients. Therefore, the aim of this study was to investigate the involvement of PKC-alpha, PKC-delta and PKC-zeta expression and activity in the signaling machinery activated in Abeta-reactive T cells, in adult healthy individuals, elderly healthy subjects, and from patients with AD. The results show that in peripheral T-cells from early AD patients, Abeta(1-42) produced a distinct subpopulation highly expressing P-PKC-delta, while in severe AD patients the same treatment induced two distinct P-PKC-delta and P-PKC-zeta T-cell subpopulations. Such subpopulations were not noticeable following CD3/CD28 treatment of the same samples or after treatment of peripheral T cells from healthy adult or elderly subjects with Abeta(1-42) or with CD3/CD28. We believe that these findings may be of help in possible attempts to develop further diagnostic strategies useful for the characterization of AD.
Miscia
Sebastiano
S
Cell Signalling Unit, Department of Biomorphology, University G. d'Annunzio of Chieti-Pescara, Chieti, Italy. s.miscia@morpho.unich.it
Ciccocioppo
Fausta
F
Lanuti
Paola
P
Velluto
Lucia
L
Bascelli
Adriana
A
Pierdomenico
Laura
L
Genovesi
Domenico
D
Di Siena
Alessandro
A
Santavenere
Eugenio
E
Gambi
Francesco
F
Ausili-Cèfaro
Giampiero
G
Grimley
Philip M
PM
Marchisio
Marco
M
Gambi
Domenico
D
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000201:enzymology* / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000502:physiology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000502:physiology*; D011493:Protein Kinase C / Q000096:biosynthesis* / Q000502:physiology; D051571:Protein Kinase C-alpha / Q000096:biosynthesis / Q000502:physiology; D051745:Protein Kinase C-delta / Q000096:biosynthesis* / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000201:enzymology* / Q000473:pathology; D000099221:Protein Kinase C zeta
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D000099221:Protein Kinase C zeta; C075222:amyloid beta-protein (1-42)