Increased glycation of hemoglobin and plasma proteins in normotensive, non-diabetic obese Indian subjects: putative role of lipid peroxides.
BACKGROUND
Glycation and lipid peroxidation are spontaneous reactions believed to contribute to the pathogenesis of many clinical disorders. The purpose of the present study was to evaluate the levels of lipid peroxides and glycated proteins in normotensive, non-diabetic obese Indian subjects and to assess possible associations between them.


METHODS
A total of 28 obese male subjects and 20 non-obese subjects were included in the present study. Whole blood glycated hemoglobin, plasma lipid peroxides and fructosamine levels were estimated in both groups.


RESULTS
Lipid peroxides, glycated hemoglobin and fructosamine levels were significantly higher in obese subjects in comparison with non-obese subjects. We also found a significant association between malondialdehyde and body mass index (r=0.424, p=0.025). Partial correlation analysis revealed that malondialdehyde was significantly correlated with glycated hemoglobin (r=0.590, p=0.01) and fructosamine (r=0.442, p=0.021) after controlling for glucose.


CONCLUSIONS
Increased glycation of proteins was found in normotensive, non-diabetic obese Indian subjects. These data also support the premise that lipid peroxides per se play a role in the glycation of hemoglobin and plasma proteins.
Sathiyapriya
Viswanathan
V
Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India.
Selvaraj
Nambiar
N
Nandeesha
Hanumanthappa
H
Bobby
Zachariah
Z
Agrawal
Aparna
A
Sridhar
Magadi Gopalakrishna
MG
Pavithran
Purushothaman
P
Rattina Dasse
Nadaradjan
N
D000328:Adult; D001798:Blood Proteins / Q000032:analysis / Q000378:metabolism*; D015992:Body Mass Index; D016022:Case-Control Studies; D019270:Fructosamine / Q000097:blood; D006031:Glycosylation; D006454:Hemoglobins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007194:India; D008054:Lipid Peroxides / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism*; D011499:Protein Processing, Post-Translational
D001798:Blood Proteins; D006454:Hemoglobins; D008054:Lipid Peroxides; D019270:Fructosamine; D008315:Malondialdehyde