title: p53AIP1 expression can be a prognostic marker in non-small cell lung cancer.
abstract: OBJECTIVE
  p53AIP1 is a potential mediator of p53-dependent apoptosis that is mutated in many kinds of carcinoma. To investigate the role of this gene for non-small cell lung cancer, we compared the relationship between p53AIP1 gene expression and clinicopathological status of lung cancer.
  
  
  METHODS
  Seventy samples from non-small cell lung cancer patients were obtained between 1997 and 2003. For quantitative evaluation of RNA expression by polymerase chain reaction (PCR) we used the Taqman PCR methods. Exons 5-8 of the p53 gene were analysed using PCR-single-stranded conformation polymorphism and sequenced for mutation analysis.
  
  
  RESULTS
  p53AIP1 gene expression levels in the lymph node metastasis-positive group were significantly lower than in the negative group (positive 35.1+/-83.9; negative 64.2+/-113.4; P=0.0486). The overall survival of the p53AIP1 low expression group was significantly worse than that of the p53AIP1 high expression group (P=0.0206). In the multivariate Cox proportional hazard model, p53AIP1 (P=0.0489) was the independent predictor for overall survival. When we investigated mutation analyses of the p53 gene, we could find several point mutations in 15.7% of all samples. However, there was no relationship between p53AIP1 expression and p53 status.
  
  
  CONCLUSIONS
  These data suggest that the p53AIP1 gene is important for non-small cell lung cancer progression and may be a possible prognostic marker.
authors.affiliation: Department of Surgery, National Hospital Organization Kumamoto Medical Center, 1-5 Ninomaru, Kumamoto, 860-0008, Japan. yamashi1@med.oita-u.ac.jp
authors.forename: S-I
authors.initials: SI
authors.lastname: Yamashita
authors.forename: Y
authors.initials: Y
authors.lastname: Masuda
authors.forename: N
authors.initials: N
authors.lastname: Yoshida
authors.forename: H
authors.initials: H
authors.lastname: Matsuzaki
authors.forename: T
authors.initials: T
authors.lastname: Kurizaki
authors.forename: Y
authors.initials: Y
authors.lastname: Haga
authors.forename: S
authors.initials: S
authors.lastname: Ikei
authors.forename: M
authors.initials: M
authors.lastname: Miyawaki
authors.forename: Y
authors.initials: Y
authors.lastname: Kawano
authors.forename: M
authors.initials: M
authors.lastname: Chujyo
authors.forename: K
authors.initials: K
authors.lastname: Kawahara
mesh_terms: D000368:Aged; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D016158:Genes, p53; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008207:Lymphatic Metastasis; D008297:Male; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011379:Prognosis; D012907:Smoking / Q000009:adverse effects; D016019:Survival Analysis
chemical_list: D051017:Apoptosis Regulatory Proteins; C414742:P53AIP1 protein, human