title: Development and characterization of a continuous cell line, AFKM-On-H, from hemocytes of the European corn borer Ostrinia nubilalis (Hübner) (Lepidoptera, Pyralidae).
abstract: The corn borer, Ostrinia nubilalis, is a very important pest in different countries, and the in vitro system of the insect could be a useful tool for isolation and characterization of the pathogens and physiological responses of the insect. In this context, a cell line was derived from the hemocytes of the European corn borer and was named AFKM-On-H for, respectively, O. nubilalis, Armand Frappier, King Mongkut Institutes, and Hemocytes. This cell line was initiated and maintained in Ex-Cell 400 medium supplemented with 10% heat-inactivated fetal bovine serum. The cells, mostly spherical in shape, not firmly attached to the plastic culture flasks, were passaged up to 200 times by repeated gentle pipetting of the cells. The doubling times at the 80th and 125th passages at 28 degrees C and at the 122th and 169th passages at 25 degrees C were 40, 29, 35, and 34 h, respectively. The AFKM-On-H cell line was further characterized by the morphology, karyotype, random amplified polymorphic DNA analysis, and isozyme profiles. Susceptibility of the cell line to cytoplasmic polyhedrosis viruses (CPV) Euxoa scandens (EsCPV), Dendrolimus punctatus (DpCPV), and Choristoneura fumiferana (CfCPV); nuclear polyhedrosis viruses [Autographa californica (AcMNPV) wild type and recombinant, Antherea yammamai (AnyaNPV)]; and Chilo iridescent virus was demonstrated. Relative sensitivities of the cell line to Bacillus thuringiensis and Metarhizium anisopliae toxins and effects of the molting hormone 20-hydroxyecdysone on this new hemocyte cell line were characterized.
authors.affiliation: INRS-Institut Armand-Frappier, Université du Québec, 531 boulevard des prairies, Laval, Quebec, Canada. serge_belloncik@yahoo.com
authors.forename: S
authors.initials: S
authors.lastname: Belloncik
authors.forename: O
authors.initials: O
authors.lastname: Petcharawan
authors.forename: M
authors.initials: M
authors.lastname: Couillard
authors.forename: G
authors.initials: G
authors.lastname: Charpentier
authors.forename: B
authors.initials: B
authors.lastname: Larue
authors.forename: H
authors.initials: H
authors.lastname: Guardado
authors.forename: S
authors.initials: S
authors.lastname: Chareonsak
authors.forename: S
authors.initials: S
authors.lastname: Imanishi
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology / Q000633:toxicity; D002417:Cattle; D002460:Cell Line; D048430:Cell Shape; D004441:Ecdysterone / Q000494:pharmacology; D005819:Genetic Markers; D006434:Hemocytes / Q000166:cytology* / Q000187:drug effects / Q000821:virology; D007313:Insecta; D007621:Karyotyping; D010944:Plants / Q000469:parasitology
chemical_list: D001427:Bacterial Toxins; D005819:Genetic Markers; D004441:Ecdysterone