title: Hypertonic saline solution stimulated CLC-3 production in primary cultured human nasal epithelial cells.
abstract: OBJECTIVE
  To determine the molecular mechanisms of hypertonic saline on nasal disorders.
  
  
  METHODS
  Primary human nasal epithelial cells (HNECs) were treated with 0.9 and 3% saline for 12h. CLC-3 was detected in HNECs by immunohistochemistry, reverse transcriptase-polymerase chain reaction (RT-PCR) and western blotting.
  
  
  RESULTS
  Positive expression of CLC-3 was detected in HNECs in all subjects by immunohistochemistry. The relative density of CLC-3 mRNA was 0.22+/-0.09 and 0.12+/-0.05 in HNECs treated with 3 and 0.9% saline, respectively. The effect of 3% saline on the expression of CLC-3 in HNECs was observed in a time-dependent manner.
  
  
  CONCLUSIONS
  Hypertonic saline decreases the nasal edema and influences the mucocilliary function through the fluid secretion in vitro and may be associated with the upregulation of CLC-3.
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Hua-Bin
authors.initials: HB
authors.lastname: Li
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Shi
authors.forename: Fang
authors.initials: F
authors.lastname: Cao
authors.forename: Lei
authors.initials: L
authors.lastname: Cheng
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Wen
authors.forename: Geng
authors.initials: G
authors.lastname: Xu
mesh_terms: D000328:Adult; D015153:Blotting, Western; D002478:Cells, Cultured; D018118:Chloride Channels / Q000187:drug effects* / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D005260:Female; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000187:drug effects* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D018118:Chloride Channels; C115376:ClC-3 channel; D012333:RNA, Messenger; D012462:Saline Solution, Hypertonic