High-resolution water window X-ray imaging of in vivo cells and their products using LiF crystal detectors.
High contrast imaging of in vivo Chlorella sorokiniana cells with submicron spatial resolution was obtained with a contact water window X-ray microscopy technique using a point-like, laser-plasma produced, water-window X-ray radiation source, and LiF crystals as detectors. This novel type of X-ray imaging detectors is based on photoluminescence of stable electronic point defects, characterized by high intrinsic resolution. The fluorescence images obtained on LiF crystals exposed in single-shot experiments demonstrate the high sensitivity and dynamic range of this new detector. The powerful performances of LiF crystals allowed us to detect the exudates of Chlorella cells in their living medium and their spatial distribution in situ, without any special sample preparation.
Bonfigli
Francesca
F
Physics Technologies and New Materials Department, ENEA, CR Frascati, 00044 Frascati, Rome, Italy.
Faenov
Anatoly
A
Flora
Francesco
F
Francucci
Massimo
M
Gaudio
Pasqualino
P
Lai
Antonia
A
Martellucci
Sergio
S
Montereali
Rosa Maria
RM
Pikuz
Tania
T
Reale
Lucia
L
Richetta
Maria
M
Vincenti
Maria Aurora
MA
Baldacchini
Giuseppe
G
D002708:Chlorella / Q000166:cytology* / Q000378:metabolism; D003460:Crystallization; D005459:Fluorides / Q000737:chemistry*; D018020:Lithium Compounds / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D014965:X-Rays
D018020:Lithium Compounds; C027651:lithium fluoride; D005459:Fluorides