Comparison of urinary and recombinant follicle-stimulating hormone in in vitro growth, maturation, and fertilization of mouse preantral follicles.
OBJECTIVE
To compare the effects of urinary and recombinant follicle-stimulating hormone (FSH) on follicular development, oocyte maturation, and fertilization.


METHODS
Prospective randomized animal study.


METHODS
University-based research laboratory.


METHODS
Normal (C57BL/6xDBA2) F1 mice.


METHODS
Collection of preantral follicles by mechanical dissection, in vitro growth (IVG) with urinary or recombinant FSH (100 mIU/mL), in vitro maturation (IVM), and fertilization.


METHODS
During IVG, follicle diameter and antral formation were evaluated. The number of cells per follicle was evaluated at the end of IVG. The 17beta-estradiol measurements were performed from conditioned media. After IVM, mucification and oocyte maturation rates were estimated and mature oocytes were fertilized.


RESULTS
No differences were observed in the antral formation rate of either group. Antral follicles in the urinary FSH group, however, showed a higher diameter but a lower number of cells per follicle than those in the recombinant FSH group. The level of 17beta-estradiol was also higher in the recombinant FSH group. The rate of mature oocytes developing into metaphase I or II was statistically significantly higher in the recombinant FSH group than in the urinary FSH group.


CONCLUSIONS
Recombinant FSH provided better conditions than urinary FSH for the growth and development of mouse preantral follicles that could produce mature oocytes with fertilization capacity.
Calongos
Giannina
G
Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Japan.
Hasegawa
Akiko
A
Komori
Shinji
S
Koyama
Koji
K
D000818:Animals; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D002970:Cleavage Stage, Ovum / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D008297:Male; D008596:Menotropins / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009866:Oogenesis / Q000187:drug effects*; D006080:Ovarian Follicle / Q000187:drug effects* / Q000254:growth & development*; D011247:Pregnancy; D011994:Recombinant Proteins / Q000494:pharmacology
D011994:Recombinant Proteins; D004958:Estradiol; D008596:Menotropins; D005640:Follicle Stimulating Hormone