title: Conjugation of polyamidoamine dendrimers on biodegradable microparticles for nonviral gene delivery.
abstract: We report on the preparation and characterization of poly(D, L-lactide-co-glycolide) (PLGA) microparticles with surface-conjugated polyamidoamine (PAMAM) dendrimers of varying generations. The buffering capacity and zeta-potential of the PLGA PAMAM microparticles increased with increasing generation level of the PAMAM dendrimer conjugated. Conjugation of the PAMAM dendrimer to the surface of the PLGA microparticle removed generation-dependent cytotoxicity in HEK293 and COS7 cell lines. PLGA PAMAM pDNA microparticles displayed similar cytotoxicity profiles to unmodified PLGA pDNA microparticles in COS7 cells. A generation three PAMAM dendrimer conjugated to PLGA microparticles significantly increased transfection efficiencies in comparison to unmodified PLGA microparticles.
authors.affiliation: Division of Pharmaceutics, College of Pharmacy, University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Janjira
authors.initials: J
authors.lastname: Intra
authors.forename: Aliasger K
authors.initials: AK
authors.lastname: Salem
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000235:genetics; D050091:Dendrimers / Q000737:chemistry* / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D010316:Particle Size; D010957:Plasmids / Q000235:genetics; D011073:Polyamines / Q000737:chemistry*; D013499:Surface Properties; D014162:Transfection
chemical_list: D004279:DNA, Viral; D050091:Dendrimers; C104700:PAMAM Starburst; D011073:Polyamines