In vitro study on influence of a discrete nano-hydroxyapatite on leukemia P388 cell behavior.
Influence of a discrete nano-hydroxyapatite crystal (nano-HAp) on lymphatic leukemia P388 cell behavior was investigated by an in vitro technique using an MTT test and FITC analysis. The discrete nano-HAp was prepared by decanting upside clear layer of the nano-HAp suspension which was synthesized by a wet method using calcium hydroxide suspension and phosphoric acid solutions. The nano-HAp was identified as hydroxyapatite by the X-ray powder diffraction pattern and an infrared spectroscopy. The nano-HAp with concentrations of 8-40 microg/ml was dispersed into PRMI 1640 media with leukemia cells derived from BALB/C mice. The survival ratio of the cells decreased with the amounts of the nano-HAp increasing. Apoptosis rates of leukemia P388 cells on co-culturing with the nano-HAp of 35 microg/ml for 24, 48 h were 14.5% and 45.8%, respectively, and higher than those of 10.4% and 34% in controls. The G0/G1 peak values in leukemia P388 cell cycle were declined to reduce and the S peak values increased with extension time of co-culture. These results proved that the discrete nano-HAp can cause apoptosis of the leukemia P388 cells, and selectively act on G1 phase and arrest the G1 phase in cell cycle of the leukemia P388.
Li
G
G
Department of Pediatrics, Sichuan Provincial People's Hospital, Chengdu, P. R. China. ligejp2002@yahoo.com.cn
Huang
J
J
Li
Y
Y
Zhang
R
R
Deng
B
B
Zhang
J
J
Aoki
H
H
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D003094:Collagen / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D017886:Durapatite / Q000008:administration & dosage*; D007941:Leukemia P388 / Q000473:pathology*; D051379:Mice; D010316:Particle Size
D001672:Biocompatible Materials; C509317:nano-hydroxyapatite-collagen; D003094:Collagen; D017886:Durapatite