Emulsion-based synthesis of PLGA-microspheres for the in vitro expansion of porcine chondrocytes.
The in vitro cell expansion of autologous chondrocytes is of high interest in regenerative medicine since these cells can be used to treat joint cartilage defects. In order to preserve chondrocyte phenotype, while optimizing adhesion on microspheres, several processing parameters for the microsphere synthesis were varied. In this study three different polylactide-co-glycolides were used with differing lactide-glycolide ratios (85:15 and 50:50) and differing inherent viscosities. An emulsion route was established, where the polymer was dissolved in chloroform and then injected into a stirred polyvinyl alcohol-water solution at different polymer concentrations and different stirring velocities to produce microspheres with varying diameters. The sphere size distribution and morphology was analyzed using image processing software on SEM pictures. Based on previous experiments with commercial microspheres, three optimum samples were selected for further investigations. The degradation of the microspheres was determined in a long-term experiment in culture medium for 3 months. Adherent cells were characterized after 3 and 5 days by FDA+EB vital staining and in SEM.
Gabler
Franziska
F
Technical University Berlin, Institute of Biotechnology, Ackerstrasse 71-76, Berlin 13355, Germany.
Frauenschuh
Simone
S
Ringe
Jochen
J
Brochhausen
Christoph
C
Götz
Peter
P
Kirkpatrick
C James
CJ
Sittinger
Michael
M
Schubert
Helmut
H
Zehbe
Rolf
R
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D004655:Emulsions / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D008863:Microspheres; D010316:Particle Size; D011100:Polyglycolic Acid / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D011142:Polyvinyl Alcohol / Q000737:chemistry; D013499:Surface Properties; D013552:Swine; D013995:Time; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D004655:Emulsions; D011108:Polymers; D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011142:Polyvinyl Alcohol