title: Fat storage is partially dependent on vagal activity and insulin secretion of hypothalamic obese rat.
abstract: Hypothalamic MSG-obese rats show hyperinsulinemia and tissue insulin resistance, and they display intense parasympathetic activity. Current analysis investigates whether early subdiaphragmatic vagotomy prevents tissue insulin sensitivity impairment in adult obese MSG-rats. Hypothalamic obesity was induced by MSG (4 mg/g BW), daily, from birth up to 5 days. Control animals receiving saline solution. On the 30th day rats underwent bilateral subdiaphragmatic vagotomy or sham surgery. An intravenous glucose tolerance test (i.v.GTT) was performed when rats turned 90 days old. Total white fat tissue (WAT) from rat carcass was extracted and isolated; the interscapular brown fat tissue (IBAT) was weighed. Rather than blocking obesity, vagotomy reduced WAT and IBAT in MSG-obese rats when the latter were compared to sham MSG-rats. High blood fasting insulin and normal glucose levels were also observed in MSG-obese rats. Although glucose intolerance, high insulin secretion, and significant insulin resistance were recorded, vagotomy improved fasting insulinemia, glucose tolerance and insulin tissue sensitivity in MSG-obese rats. Results suggest that increased fat accumulation is caused, at least in part, by high blood insulin concentration, and enhanced parasympathetic activity on MSG-obese rats.
authors.affiliation: Laboratory of Secretion Cell Biology, Department of Cell Biology and Genetics, State University of Maringá, Avenida Colombo 5790, Maringa, PR, 87020-900, Brazil.
authors.forename: Sandra Lucinei
authors.initials: SL
authors.lastname: Balbo
authors.forename: Sabrina
authors.initials: S
authors.lastname: Grassiolli
authors.forename: Rosane Aparecida
authors.initials: RA
authors.lastname: Ribeiro
authors.forename: Maria Lúcia
authors.initials: ML
authors.lastname: Bonfleur
authors.forename: Clarice
authors.initials: C
authors.lastname: Gravena
authors.forename: Marcia do Nascimento
authors.initials: Mdo N
authors.lastname: Brito
authors.forename: Ana Eliza
authors.initials: AE
authors.lastname: Andreazzi
authors.forename: Paulo Cezar de Freitas
authors.initials: PC
authors.lastname: Mathias
authors.forename: Rosana
authors.initials: R
authors.lastname: Torrezan
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D050154:Adiposity / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D018149:Glucose Intolerance / Q000209:etiology; D005951:Glucose Tolerance Test; D048788:Growth and Development / Q000187:drug effects; D007027:Hypothalamic Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D007328:Insulin / Q000378:metabolism*; D007333:Insulin Resistance; D000078790:Insulin Secretion; D008297:Male; D009765:Obesity / Q000139:chemically induced / Q000150:complications / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D012970:Sodium Glutamate; D014628:Vagotomy; D014630:Vagus Nerve / Q000503:physiopathology*
chemical_list: D007328:Insulin; D012970:Sodium Glutamate