title: Sensitive test for screening for Staphylococcus aureus in bovine mastitis by broth cultivation and PCR.
abstract: An assay was developed and evaluated for screening for Staphylococcus aureus in milk samples from cases of bovine mastitis by overnight cultivation in a broth containing 7.5 per cent sodium chloride, followed by pcr to amplify the nuc gene. The assay could detect concentrations of S aureus as low as 1 colony-forming unit/ml milk. Among 106 milk samples collected from individual quarters of lactating cows in one dairy herd and from a bulk tank, S aureus was detected in nine samples by the pcr assay but in only three samples by conventional microbiological culture.
authors.affiliation: Research Unit for Food Animal Medicine, Research Centre for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yamagishi
authors.forename: Y
authors.initials: Y
authors.lastname: Jinkawa
authors.forename: K
authors.initials: K
authors.lastname: Omoe
authors.forename: S
authors.initials: S
authors.lastname: Makino
authors.forename: K
authors.initials: K
authors.lastname: Oboshi
mesh_terms: D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial / Q000379:methods / Q000662:veterinary*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005784:Gene Amplification; D008403:Mass Screening / Q000379:methods / Q000592:standards / Q000662:veterinary*; D008414:Mastitis, Bovine / Q000175:diagnosis*; D008892:Milk / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial