title: Transfer printing of DNA by "click" chemistry.
abstract: This paper describes a straightforward procedure to immobilize oligonucleotides on glass substrates in well-defined micropatterns by microcontact printing with a dendrimer-modified stamp. The oligonucleotides are efficiently immobilized by "click" chemistry induced by microcontact printing. Acetylene-modified oligonucleotides were treated with an azide-terminated glass slide under the confinement of the dendrimer-modified stamp, without the use of a Cu(I) catalyst. The immobilization is an irreversible, covalent, and one-step reaction that results in stable attachment of the oligonucleotides. Oligonucleotides with the acetylene-modification at the 5' terminus hybridize selectively with full-length, complementary targets. Strands with more than one acetylene linker do not hybridize with complementary strands.
authors.affiliation: Laboratory of Supramolecular Chemistry and Technology, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
authors.forename: Dorota I
authors.initials: DI
authors.lastname: Rozkiewicz
authors.forename: Johannes
authors.initials: J
authors.lastname: Gierlich
authors.forename: Glenn A
authors.initials: GA
authors.lastname: Burley
authors.forename: Katrin
authors.initials: K
authors.lastname: Gutsmiedl
authors.forename: Thomas
authors.initials: T
authors.lastname: Carell
authors.forename: Bart Jan
authors.initials: BJ
authors.lastname: Ravoo
authors.forename: David N
authors.initials: DN
authors.lastname: Reinhoudt
mesh_terms: D000114:Acetylene / Q000737:chemistry; D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D008968:Molecular Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D010316:Particle Size; D013329:Structure-Activity Relationship; D013499:Surface Properties; D013844:Thiazoles / Q000737:chemistry
chemical_list: D050091:Dendrimers; D008567:Membranes, Artificial; D009841:Oligonucleotides; C086329:TOTO-1 thiazole orange dye; D013844:Thiazoles; D004247:DNA; D000114:Acetylene