title: Developmental regulation of SSeCKS expression in rat brain.
abstract: SSeCKS (src suppressed C kinase substrate) was identified as a PKC substrate/PKC-binding protein, which plays a role in mitogenic regulatory activity and has a function in the control of cell signaling and cytoskeletal arrangement. Previous studies showed that expression of SSeCKS mRNA and protein levels were developmentally regulated in rat testis and the molecular might have some effects on the process of spermiogenesis. Here we carried out experiments to investigate the expression of SSeCKS in rat brain. Western blot analysis indicated that SSeCKS could be detected in the whole brain of developing rat embryos and reached its peak at 1 week after birth, while during mature period, its level was decreasing. Regional-distribution analysis showed that the expression pattern of SSeCKS in telencephalon, hippocampus and diencephalons was in accordance with the result from whole brain both in mRNA and protein level. However, in cerebellum, SSeCKS was almost in the same level, and in brainstem, the expression level was higher in 4-week-old rat brain than in 1-week-old one. Immunohistochemistry results showed SSeCKS was in diffused and granule-like distribution. Double immunofluorescence staining showed that it was expressed by some GFAP positive cells. All the results suggested that SSeCKS might affect brain development and further research is needed to have a good understanding of its function and mechanism.
authors.affiliation: Department of Pathology, Medical School of Nantong University (Former Nantong Medical College), Nantong 226001, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Qin
authors.forename: Chun
authors.initials: C
authors.lastname: Cheng
authors.forename: Hai'ou
authors.initials: H
authors.lastname: Liu
authors.forename: Shuqiong
authors.initials: S
authors.lastname: Niu
authors.forename: Ji
authors.initials: J
authors.lastname: Qian
authors.forename: Linlin
authors.initials: L
authors.lastname: Sun
authors.forename: Feng
authors.initials: F
authors.lastname: Xiao
authors.forename: Shuxian
authors.initials: S
authors.lastname: Shi
authors.forename: Aiguo
authors.initials: A
authors.lastname: Shen
mesh_terms: D054758:A Kinase Anchor Proteins; D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000196:embryology / Q000254:growth & development* / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D054758:A Kinase Anchor Proteins; C102361:Akap12 protein, rat; D015415:Biomarkers; D018797:Cell Cycle Proteins; D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger