title: Analysis of de novo Golgi complex formation after enzyme-based inactivation.
abstract: The Golgi complex is characterized by its unique morphology of closely apposed flattened cisternae that persists despite the large quantity of lipids and proteins that transit bidirectionally. Whether such a structure is maintained through endoplasmic reticulum (ER)-based recycling and auto-organization or whether it depends on a permanent Golgi structure is strongly debated. To further study Golgi maintenance in interphase cells, we developed a method allowing for a drug-free inactivation of Golgi dynamics and function in living cells. After Golgi inactivation, a new Golgi-like structure, containing only certain Golgi markers and newly synthesized cargoes, was produced. However, this structure did not acquire a normal Golgi architecture and was unable to ensure a normal trafficking activity. This suggests an integrative model for Golgi maintenance in interphase where the ER is able to autonomously produce Golgi-like structures that need pre-existing Golgi complexes to be organized as morphologically normal and active Golgi elements.
authors.affiliation: Centre National de la Recherche Scientifique Unité Mixte de Recherche 144, 75248 Paris Cedex 05, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Jollivet
authors.forename: Graça
authors.initials: G
authors.lastname: Raposo
authors.forename: Ariane
authors.initials: A
authors.lastname: Dimitrov
authors.forename: Rachid
authors.initials: R
authors.lastname: Sougrat
authors.forename: Bruno
authors.initials: B
authors.lastname: Goud
authors.forename: Franck
authors.initials: F
authors.lastname: Perez
mesh_terms: D015100:3,3'-Diaminobenzidine / Q000494:pharmacology; D002470:Cell Survival; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004789:Enzyme Activation; D006056:Golgi Apparatus / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology* / Q000648:ultrastructure; D006367:HeLa Cells; D006735:Horseradish Peroxidase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007399:Interphase; D008854:Microscopy, Electron; D011485:Protein Binding
chemical_list: D015100:3,3'-Diaminobenzidine; D006735:Horseradish Peroxidase