title: [Study on the molecular typing and epidemiology of non-polio enteroviruses isolated from Yunnan province, China].
abstract: OBJECTIVE
  This report presented an overview on the epidemiology of enterovirus in Yunnan province, the People's Republic of China.
  
  
  METHODS
  A total of 210 strains of non-polioviruses isolated under acute flaccid paralysis surveillance during a 5-year study period from 1997 to 2000 and 2004 were examined. Of the 210 non-polioviruses strains, a total of 12 strains of adenoviruses were serologically identified. The remaining 198 isolates were used for molecular typing, and the viral genomes of 195 nonpolio enteroviruses (NPEVs) were translated to corresponding amino acid sequences and compared with those of the prototype strains.
  
  
  RESULTS
  Based on molecular typing, 5 isolates were classified into 5 serotypes of human enterovirus A species while 158 isolates into 34 serotypes of B and 32 isolates into 6 serotypes of C species. However, we did not isolate any viruses which belonged to human enterovirus D species. Thus, under acute flaccid paralysis surveillance, human enterovirus B species accounted for 75.2% of the 210 isolates and was considered as the predominant one, followed by human enterovirus C (12.2%), adenovirus (5.7%), and human enterovirus A (2.4%).
  
  
  CONCLUSIONS
  Although the epidemiological characteristics of NPEVs from Yunnan province remained "unknown", the molecular typing method had provided us a breakthrough to understand the epidemiology of these viruses.
authors.affiliation: Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China.
authors.forename: Bing-jun
authors.initials: BJ
authors.lastname: Tian
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Dong-hua
authors.initials: DH
authors.lastname: Zhang
authors.forename: Li-fang
authors.initials: LF
authors.lastname: He
authors.forename: Zheng-rong
authors.initials: ZR
authors.lastname: Ding
authors.forename: Lin
authors.initials: L
authors.lastname: Lu
mesh_terms: D002681:China / Q000453:epidemiology; D004770:Enterovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000453:epidemiology*; D005814:Genes, Viral; D006801:Humans; D017720:Molecular Epidemiology; D012703:Serotyping