Cloning and expression pattern of SsHKT1 encoding a putative cation transporter from halophyte Suaeda salsa.
Potassium is an essential element for plant, and high-affinity K+ uptake system plays a crucial role in potassium absorption and transportation. Here we report the isolation and characterization of a HKT1 homolog from C3 halophyte Suaeda salsa (L.) (SsHKT1), particularly under low K+ treatment. The SsHKT1 cDNA was 2033 nucleotides long including 1650 bp ORF for a 550 amino acids peptide and a predicted molecular mass of 63.0 kDa. The deduced amino acid sequence of SsHKT1 was 39-64% identical to other plant HKT-like sequences. A SsHKT1-specific antibody was prepared and reacted with a 63.0 kDa protein from S. salsa plasma membrane. Reverse transcriptase-PCR analysis showed that SsHKT1 was mainly expressed in leaf tissues and to a lesser extent, in root tissues. Amounts of SsHKT1 transcript were developmentally controlled and significantly up-regulated by K+ deprivation and NaCl treatment. The results suggested that SsHKT1 might play an important role in ion homeostasis and salt tolerance of S. salsa.
Shao
Qun
Q
Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan 250014, P R China.
Zhao
Chang
C
Han
Ning
N
Wang
Bao-shan
BS
D000595:Amino Acid Sequence; D027682:Cation Transport Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D019663:Chenopodiaceae / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis / Q000302:isolation & purification; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D012462:Saline Solution, Hypertonic; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D027981:Symporters / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*
D027682:Cation Transport Proteins; D018076:DNA, Complementary; C088864:HKT1 protein, plant; D010940:Plant Proteins; D012462:Saline Solution, Hypertonic; D027981:Symporters