title: Eye-open at birth phenotype with reduced keratinocyte motility in LGR4 null mice.
abstract: We observed a consistent eye-open at birth (EOB) phenotype in mouse pups homozygous for a leucine-rich repeat containing G-protein coupled receptor 4 (Lgr4) allele deleting the whole transmembrane domain coding region. An in vitro wound-healing scratch assay showed notably reduced keratinocyte motility in the null mice. Phalloidin staining of F-actin in the eyelid epidermis was also reduced. We also generated keratinocyte-specific Lgr4 deficient mice, circumventing the embryonic/neonatal lethality and kidney abnormalities. Most of the conditional Lgr4 knockout mice showed the EOB phenotype. Thus, Lgr4 might be a novel gene class regulating cell motility.
authors.affiliation: Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Kato
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Mohri
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Matsuo
authors.forename: Eisaku
authors.initials: E
authors.lastname: Ogawa
authors.forename: Akihiro
authors.initials: A
authors.lastname: Umezawa
authors.forename: Ryuhei
authors.initials: R
authors.lastname: Okuyama
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Nishimori
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002470:Cell Survival; D002478:Cells, Cultured; D004628:Embryonic Structures / Q000196:embryology / Q000378:metabolism; D005123:Eye / Q000196:embryology / Q000378:metabolism / Q000503:physiopathology*; D005124:Eye Abnormalities / Q000235:genetics* / Q000503:physiopathology*; D018507:Gene Expression Regulation, Developmental; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D010641:Phenotype; D043562:Receptors, G-Protein-Coupled / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*
chemical_list: C496200:LGR4 protein, mouse; D043562:Receptors, G-Protein-Coupled