A functional analysis of KlSRB10: implications in Kluyveromyces lactis transcriptional regulation.
The function of KlSRB10 has been studied by diverse approaches. Primer extension analysis reveals several transcription start sites, position - 17 from ATG being predominant. Deletion of KlSRB10 diminishes growth in ethanol and decreases KlCYC1 transcript levels. A second phenotype associated with this deletion affects growth in galactose. These phenotypes are independent of the specific sequence connecting the ATP binding cassette and the kinase domain of Srb10p in yeasts. KlSrb10p is not necessary for LAC4 repression mediated by KlGal80p, as deduced by construction of a Klgal80Deltasrb10Delta double mutant. In the two-hybrid system, KlSrbp10p interacts with the protein encoded by KLLA0E08151g (KlSrbp11p).
Núñez
Laura
L
Department of Molecular and Cell Biology, University of A Coruña, Campus da Zapateira, s/n 15071-A Coruña, Spain.
González-Siso
Isabel
I
Rodríguez-Belmonte
Esther
E
Soengas
Pilar
P
Lamas-Maceiras
Mónica
M
Cerdán
Maria Esperanza
ME
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques
D004271:DNA, Fungal; D012331:RNA, Fungal; D018844:Cyclin-Dependent Kinases