title: FGF-4 increases in vitro expansion rate of human adult bone marrow-derived mesenchymal stem cells.
abstract: Human bone marrow-derived mesenchymal stem cells (MSCs) exhibit limited in vitro growth. Fibroblast growth factors (FGFs) elicit a variety of biological responses, such as cell proliferation, differentiation and migration. FGF-4 represents one of the FGFs with the highest cell mitogenic activity. We studied the effect of FGF-4 on MSCs growth and pluripotency. MSCs duplication time (Td) was significantly reduced with FGF-4 compared to controls (2.2 +/- 0.2 vs. 4.1 +/- 0.2 days, respectively; p = 0.03) while BMP-2 and SCF-1 did not exert a significant growth effect. MSC expression of surface markers, differentiation into adipogenic and osteogenic lineages, and baseline expression of cardiomyogenic genes were unaffected by FGF-4. In summary, exogenous FGF-4 increases the rate at which MSC proliferate and has no significant effect on MSC pluripotency.
authors.affiliation: Laboratory of Cell Physiology, Department of Cardiology, Institut Català de Ciencies Cardiovasculars, Hospital de la Santa Creu i Sant Pau, Universitat Autònoma de Barcelona, Sant Antoni Ma Claret 167, 08025 Barcelona, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Farré
authors.forename: Santiago
authors.initials: S
authors.lastname: Roura
authors.forename: Cristina
authors.initials: C
authors.lastname: Prat-Vidal
authors.forename: Carolina
authors.initials: C
authors.lastname: Soler-Botija
authors.forename: Anna
authors.initials: A
authors.lastname: Llach
authors.forename: Cristina E
authors.initials: CE
authors.lastname: Molina
authors.forename: Leif
authors.initials: L
authors.lastname: Hove-Madsen
authors.forename: Jordi J
authors.initials: JJ
authors.lastname: Cairó
authors.forename: Francesc
authors.initials: F
authors.lastname: Gòdia
authors.forename: Ramon
authors.initials: R
authors.lastname: Bragós
authors.forename: Juan
authors.initials: J
authors.lastname: Cinca
authors.forename: Antoni
authors.initials: A
authors.lastname: Bayes-Genis
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D049109:Cell Proliferation; D051519:Fibroblast Growth Factor 4 / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008954:Models, Biological; D032383:Myocytes, Cardiac / Q000378:metabolism; D010012:Osteogenesis; D010641:Phenotype; D013997:Time Factors
chemical_list: C496445:FGF4 protein, human; D051519:Fibroblast Growth Factor 4