[Apoptosis-modulating effect of prostaglandin E2 in coelomocytes of holothurian Eupentacta fraudatrix depends on the cell antioxidant enzyme status].
The effects of prostaglandin PGE2 on apoptosis and antioxidant enzyme activities were studied in two coelomocyte fractions of holothurian Eupentacta fraudatrix in vitro and in vivo. PGE2 (10(-8) - 10(-6) M) modulated apoptosis in a time- and concentration-dependent manner in both fractions studied in vitro. In vivo, PGE2 induced apoptosis at concentrations of 0.1-1 microg/g in the fraction enriched with morula-like cells. Phagocytes were more sensitive to the regulating effect of PGE2. In this fraction, PGE2 induced apoptosis at concentrations from 0.01 to 1 microg/g, while PGE2 at 10 microg/g demonstrated an antiapoptotic effect. In all experiments, apoptosis development was accompanied by a disbalance of the antioxidant enzyme system, primarily, decreased catalase activity.
Dolmatova
L S
LS
Zaika
O A
OA
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects*; D002374:Catalase / Q000378:metabolism*; D002478:Cells, Cultured; D015232:Dinoprostone / Q000494:pharmacology*; D047332:Holothuria / Q000166:cytology / Q000201:enzymology*; D010120:Oxytocics / Q000494:pharmacology*; D010586:Phagocytes / Q000166:cytology / Q000201:enzymology*
D000975:Antioxidants; D010120:Oxytocics; D002374:Catalase; D015232:Dinoprostone