Ca(2+)/Calmodulin-binding proteins from the C. elegans proteome.
Calmodulin (CaM) is the primary Ca(2+)-sensor that regulates a wide variety of cellular processes in eukaryotes. Although many Ca(2+)/CaM-binding proteins have been identified, very few such proteins could be found from the genome-wide protein-protein interaction maps of Caenorhabditis elegans constructed by yeast two-hybrid screening. Using a genotype-phenotype conjugation method called mRNA-display, we performed a selection for Ca(2+)/CaM-binding proteins from a proteome library of C. elegans. The method allowed the identification of 9 known and 47 previously uncharacterized Ca(2+)-dependent CaM-binding proteins from the adult worm proteome. The Ca(2+)/CaM-binding properties of these proteins were characterized and their binding motifs were identified. The availability of such information could facilitate our understanding of the signaling pathways mediated by Ca(2+)/CaM in C. elegans. Due to its simplicity and efficiency, the method could be readily applied to examine the Ca(2+)-dependent binding partners of numerous other Ca(2+)-binding proteins, which may play important roles in many signaling pathways in C. elegans.
Shen
Xinchun
X
School of Pharmacy and Carolina Center for Genome Sciences, The University of North Carolina, Chapel Hill, NC 27599, United States.
Valencia
C Alexander
CA
Gao
Weiyi
W
Cotten
Steven W
SW
Dong
Biao
B
Huang
Bao-Cheng
BC
Liu
Rihe
R
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D017173:Caenorhabditis elegans / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D008969:Molecular Sequence Data; D020543:Proteome / Q000378:metabolism; D040901:Proteomics; D017386:Sequence Homology, Amino Acid
D029742:Caenorhabditis elegans Proteins; D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D020543:Proteome; D002118:Calcium