title: [Effects of three compounds extracted from Tripterygium wilfordii Hook on angiogenesis in chick chorioallantoic membrane].
abstract: OBJECTIVE
  To investigate the effects of three compounds extracted from Tripterygium wilfordii Hook (TW) on angiogenesis in the chick chorioallantoic membrane (CAM).
  
  
  METHODS
  Fifty fresh Hongkong Mahua chicken eggs were divided into five groups: PBS-treated group, TW1-, TW2- and TW3-treated groups and Rg3-treated group. After disinfection, the eggs were incubated for six days in a constant temperature box with the temperature being controlled within 37.8 degrees C, then exposed CAM, laid the filter papers with specimen on the CAM, and the eggs were incubated for another two days. CAM was fixed with the mixture of methyl alcohol and acetone at room temperature for about 15 min, and then cutting the CAM, taking photos and observing the angiogenesis in the CAM.
  
  
  RESULTS
  There were many CAM vessels in the PBS-treated group and the blood vessel net could be seen clearly. The number of CAM vessels in the TW1-, TW2- and TW3-treated groups (10 microg/egg) was much less than that in the PBS-treated group. Furthermore, the frame of the vessels was not clear, and the color was obscure. Inhibition rates of angiogenesis in the TW1-, TW2- and TW3-treated groups were 80%, 60% and 100% respectively, while the inhibition rate of angiogenesis in the Rg3-treated group (10 microg/egg) was only 10%.
  
  
  CONCLUSIONS
  TW1, TW2 and TW3 can obviously restrain the angiogenesis in CAM and still need further study.
authors.affiliation: Teaching and Research Group of Chinese Materia Madica, School of Chinese Materia Madica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. zhuguofush@yahoo.com.cn
authors.forename: Guo-fu
authors.initials: GF
authors.lastname: Zhu
authors.forename: Hui-qing
authors.initials: HQ
authors.lastname: Zhang
authors.forename: Ai-jun
authors.initials: AJ
authors.lastname: Hou
authors.forename: Yan-long
authors.initials: YL
authors.lastname: Yang
authors.forename: Qiu-shuang
authors.initials: QS
authors.lastname: Li
authors.forename: Cong-cong
authors.initials: CC
authors.lastname: Zhang
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply*; D010936:Plant Extracts / Q000494:pharmacology*; D011897:Random Allocation; D029757:Tripterygium / Q000737:chemistry*
chemical_list: D020533:Angiogenesis Inhibitors; D010936:Plant Extracts