Two-photon fluorescence bioimaging with an all-semiconductor laser picosecond pulse source.
We have demonstrated successful two-photon excitation fluorescence bioimaging using a high-power pulsed all-semiconductor laser. Toward this purpose, we developed a pulsed light source consisting of a mode-locked laser diode and a two-stage diode laser amplifier. This pulsed light source provided optical pulses of 5 ps duration and having a maximum peak power of over 100 W at a wavelength of 800 nm and a repetition frequency of 500 MHz.
Kuramoto
Masaru
M
SONY Corporation, Atsugi, Japan. masaru.kuramoto@jp.sony.com
Kitajima
Nobuyoshi
N
Guo
Hengchang
H
Furushima
Yuji
Y
Ikeda
Masao
M
Yokoyama
Hiroyuki
H
D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007834:Lasers; D008029:Lighting / Q000295:instrumentation* / Q000379:methods; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D012666:Semiconductors