title: Induction of apoptosis in Caco-2 and HT-29 human intestinal epithelial cells by enterohemolysin produced by classic enteropathogenic Escherichia coli.
abstract: OBJECTIVE
  Detect the cytotoxic effects of the Enterohemolysin from enteropathogenic Escherichia coli C3888 (O 26: H-) on Caco 2 and HT-29-human epithelial intestinal cells.
  
  
  RESULTS
  The Caco 2 and HT-29 cells, which were treated with Enterohemolysin (EHly) within 10-15 min, became round, lost attachment to substrate, showed extensive surface blebbing, nucleus shrank, and the chromatin became more compact. After 10 min of exposure to the EHly, the cells showed lactate dehydrogenase (LDH) leakage and reduction of mitochondrial activity. The cells showed disorganization of the actin fibers at 15 min. The death of these human epithelial intestinal cells by apoptosis was confirmed by annexin V.
  
  
  CONCLUSIONS
  Enterohemolysin induced apoptosis on human epithelial intestinal cells.
  
  
  CONCLUSIONS
  The finding of EHly cytotoxic activity suggests the involvement of this hemolysin in the (Enteropathogenic Escherichia coli) EPEC infection mechanism and may facilitate the understanding of the diarrhea caused by EPEC.
authors.affiliation: Departamento de Microbiologia e Imunologia, Instituto de Biologia, Universidade Estadual de Campinas, SP, Brazil.
authors.forename: P M S
authors.initials: PM
authors.lastname: Figueiredo
authors.forename: M T
authors.initials: MT
authors.lastname: Furumura
authors.forename: L
authors.initials: L
authors.lastname: Aidar-Ugrinovich
authors.forename: A F
authors.initials: AF
authors.lastname: Pestana de Castro
authors.forename: F G
authors.initials: FG
authors.lastname: Pereira
authors.forename: I L
authors.initials: IL
authors.lastname: Metze
authors.forename: T
authors.initials: T
authors.lastname: Yano
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D017209:Apoptosis; D018938:Caco-2 Cells; D002470:Cell Survival; D003967:Diarrhea / Q000378:metabolism / Q000382:microbiology; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D004927:Escherichia coli Infections / Q000378:metabolism* / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D019073:HT29 Cells; D006460:Hemolysin Proteins / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000382:microbiology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C066219:enterohemolysin; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase