Impacts of sterilization, microwave and ultrasonication pretreatment on hydrogen producing using waste sludge.
Hydrogen production by sterilization, microwave and ultrasonication pretreated waste sludge was investigated in this study. A new strain of Pseudomonas sp. GZ1 (EF551040) was inoculated in pretreated waste sludge to produce hydrogen. The experimental results showed that different pretreated sludge had evident differences in the yield of hydrogen production and lag time. Sterilized sludge had the largest yield of hydrogen production, and the maximum yield was 15.02 ml/gTCOD. The lag time of using sterilized sludge was 15 h, longer than other two pretreated sludge. Using the ultrasonicated sludge, the hydrogen production yield was smallest and lag time was shortest in the three pretreated sludge. Protein and carbohydrate could be released from waste sludge by pretreatment. Protein was the main nutrient used for hydrogen production. The concentration of protein, carbohydrate and SCOD increased after pretreatment and fermentation. The impacts of different pretreatments on hydrogen production were also discussed in detail.
Guo
Liang
L
College of Environmental Science and Engineering, Hunan University, Hunan, Changsha 410082, China.
Li
Xiao-Ming
XM
Bo
Xie
X
Yang
Qi
Q
Zeng
Guang-Ming
GM
Liao
De-xiang
DX
Liu
Jing-Jin
JJ
D050260:Carbohydrate Metabolism; D005232:Fatty Acids, Volatile / Q000032:analysis; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008872:Microwaves; D010100:Oxygen; D011506:Proteins / Q000378:metabolism; D012722:Sewage / Q000737:chemistry; D013242:Sterilization / Q000379:methods*; D013997:Time Factors; D014465:Ultrasonics
D005232:Fatty Acids, Volatile; D011506:Proteins; D012722:Sewage; D006859:Hydrogen; D010100:Oxygen