title: Monitoring contaminants from oil production at sea by measuring gill EROD activity in Atlantic cod (Gadus morhua).
abstract: An ex vivo gill EROD assay was applied in Atlantic cod (Gadus morhua) as a biomarker for waterborne CYP1A-inducing compounds derived from oil production at sea. Exposure to nominal concentrations of 1 ppm or 10 ppm North Sea crude oil in a static water system for 24 h caused a concentration-dependent gill EROD induction. Further, exposure of cod for 14 days to environmentally relevant concentrations of produced water (PW, diluted 1:200 or 1:1000) from a platform in the North Sea using a flow-through system resulted in a concentration-dependent induction of gill EROD. Crude oil (0.2 ppm) from the same oil field also proved to induce EROD. Finally, gill EROD activity in cod caged for 6 weeks at 500-10 000 m from two platforms outside Norway was measured. The activities in these fish were very low and did not differ from those in fish caged at reference sites.
authors.affiliation: Uppsala University, Department of Environmental Toxicology, Uppsala, Sweden. alexandra.abrahamson@ebc.uu.se
authors.forename: Alexandra
authors.initials: A
authors.lastname: Abrahamson
authors.forename: Ingvar
authors.initials: I
authors.lastname: Brandt
authors.forename: Björn
authors.initials: B
authors.lastname: Brunström
authors.forename: Rolf C
authors.initials: RC
authors.lastname: Sundt
authors.forename: Even H
authors.initials: EH
authors.lastname: Jørgensen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods; D019652:Extraction and Processing Industry; D048389:Gadus morhua / Q000378:metabolism*; D005880:Gills / Q000737:chemistry*; D006801:Humans; D009659:North Sea; D010578:Petroleum; D014877:Water Pollution, Chemical / Q000009:adverse effects*
chemical_list: D015415:Biomarkers; D010578:Petroleum; D019363:Cytochrome P-450 CYP1A1