title: High-throughput in vivo analysis of gene expression in Caenorhabditis elegans.
abstract: Using DNA sequences 5' to open reading frames, we have constructed green fluorescent protein (GFP) fusions and generated spatial and temporal tissue expression profiles for 1,886 specific genes in the nematode Caenorhabditis elegans. This effort encompasses about 10% of all genes identified in this organism. GFP-expressing wild-type animals were analyzed at each stage of development from embryo to adult. We have identified 5' DNA regions regulating expression at all developmental stages and in 38 different cell and tissue types in this organism. Among the regulatory regions identified are sequences that regulate expression in all cells, in specific tissues, in combinations of tissues, and in single cells. Most of the genes we have examined in C. elegans have human orthologs. All the images and expression pattern data generated by this project are available at WormAtlas (http://gfpweb.aecom.yu.edu/index) and through WormBase (http://www.wormbase.org).
authors.affiliation: Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Hunt-Newbury
authors.forename: Ryan
authors.initials: R
authors.lastname: Viveiros
authors.forename: Robert
authors.initials: R
authors.lastname: Johnsen
authors.forename: Allan
authors.initials: A
authors.lastname: Mah
authors.forename: Dina
authors.initials: D
authors.lastname: Anastas
authors.forename: Lily
authors.initials: L
authors.lastname: Fang
authors.forename: Erin
authors.initials: E
authors.lastname: Halfnight
authors.forename: David
authors.initials: D
authors.lastname: Lee
authors.forename: John
authors.initials: J
authors.lastname: Lin
authors.forename: Adam
authors.initials: A
authors.lastname: Lorch
authors.forename: Sheldon
authors.initials: S
authors.lastname: McKay
authors.forename: H Mark
authors.initials: HM
authors.lastname: Okada
authors.forename: Jie
authors.initials: J
authors.lastname: Pan
authors.forename: Ana K
authors.initials: AK
authors.lastname: Schulz
authors.forename: Domena
authors.initials: D
authors.lastname: Tu
authors.forename: Kim
authors.initials: K
authors.lastname: Wong
authors.forename: Z
authors.initials: Z
authors.lastname: Zhao
authors.forename: Andrey
authors.initials: A
authors.lastname: Alexeyenko
authors.forename: Thomas
authors.initials: T
authors.lastname: Burglin
authors.forename: Eric
authors.initials: E
authors.lastname: Sonnhammer
authors.forename: Ralf
authors.initials: R
authors.lastname: Schnabel
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Jones
authors.forename: Marco A
authors.initials: MA
authors.lastname: Marra
authors.forename: David L
authors.initials: DL
authors.lastname: Baillie
authors.forename: Donald G
authors.initials: DG
authors.lastname: Moerman
mesh_terms: D000367:Age Factors; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins; D011993:Recombinant Fusion Proteins; D014018:Tissue Distribution
chemical_list: D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins