A novel sodium bis(2-ethylhexyl) sulfosuccinate-PAGE system suitable for the separation of small peptides.
A novel sodium bis(2-ethylhexyl) sulfosuccinate-PAGE (AOT-PAGE) system which delivers high resolution and sensitivity for small peptides with molecular masses of 0.8-17 kDa is described. Small peptides migrate more slowly and are less prone to leakage than in conventional SDS-PAGE, thus allowing for the in-gel detection with CBB R 250 of 0.5 mug of peptide. The system is also compatible with electroblotting, activity staining in renatured gels, and the peptide analysis by MALDI-MS. AOT-PAGE is simpler, more rapid, and cheaper than the generally adopted Tricine-SDS-PAGE method.
Lu
Xuemei
X
State Key Lab of Microbial Technology, Shandong University, Jinan, PR China. luxuemei@sdu.edu.cn
Yao
Yao
Y
Zhang
Weican
W
D004143:Dioctyl Sulfosuccinic Acid / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D010455:Peptides; D004143:Dioctyl Sulfosuccinic Acid