title: Calpain activation impairs neuromuscular transmission in a mouse model of the slow-channel myasthenic syndrome.
abstract: The slow-channel myasthenic syndrome (SCS) is a hereditary disorder of the acetylcholine receptor (AChR) of the neuromuscular junction (NMJ) that leads to prolonged AChR channel opening, Ca(2+) overload, and degeneration of the NMJ. We used an SCS transgenic mouse model to investigate the role of the calcium-activated protease calpain in the pathogenesis of synaptic dysfunction in SCS. Cleavage of a fluorogenic calpain substrate was increased at the NMJ of dissociated muscle fibers. Inhibition of calpain using a calpastatin (CS) transgene improved strength and neuromuscular transmission. CS caused a 2-fold increase in the frequency of miniature endplate currents (MEPCs) and an increase in NMJ size, but MEPC amplitudes remained reduced. Persistent degeneration of the NMJ was associated with localized activation of the non-calpain protease caspase-3. This study suggests that calpain may act presynaptically to impair NMJ function in SCS but further reveals a role for other cysteine proteases whose inhibition may be of additional therapeutic benefit in SCS and other excitotoxic disorders.
authors.affiliation: Department of Neurology, University of Minnesota Medical School, Minneapolis, Minnesota, USA.
authors.forename: Jason S
authors.initials: JS
authors.lastname: Groshong
authors.forename: Melissa J
authors.initials: MJ
authors.lastname: Spencer
authors.forename: Bula J
authors.initials: BJ
authors.lastname: Bhattacharyya
authors.forename: Elena
authors.initials: E
authors.lastname: Kudryashova
authors.forename: Bhupinder P S
authors.initials: BP
authors.lastname: Vohra
authors.forename: Roberto
authors.initials: R
authors.lastname: Zayas
authors.forename: Robert L
authors.initials: RL
authors.lastname: Wollmann
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Miller
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Gomez
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D002154:Calpain / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004195:Disease Models, Animal; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000201:enzymology*; D020294:Myasthenic Syndromes, Congenital / Q000201:enzymology / Q000503:physiopathology*; D009469:Neuromuscular Junction / Q000201:enzymology*; D009435:Synaptic Transmission / Q000187:drug effects
chemical_list: D002135:Calcium-Binding Proteins; C033668:calpastatin; D002154:Calpain