Differing effects of water-soluble and fat-soluble extracts from Japanese radish (Raphanus sativus) sprouts on carbohydrate and lipid metabolism in normal and streptozotocin-induced diabetic rats.
We have shown previously that Japanese radish (Raphanus sativus) sprouts (JRS) improve blood glucose levels in diabetic rats. In this study, we investigated the components in JRS that caused this hypoglycemic effect, by examining the effects of water-soluble (WSE) and fat-soluble (FSE) extracts of JRS on diabetes markers in normal (NM) and streptozotocin (STZ)-induced diabetic (DM) rats. The NM and DM rats were divided into a control group and 2 test groups (WSE (2.2%) or FSE (0.2%)), with the rats (n = 6/group) then being maintained for 3 wk on either a control diet or one of the test diets; this was followed by the measurement of serum concentrations of glucose, insulin, glycoalbumin, fructosamine, ketone bodies, and lipids (cholesterol and triglyceride) and liver concentrations of lipids (total lipid, total cholesterol, and triglyceride). The FSE suppressed insulin secretion and improved lipid metabolism in the NM rats. The effect of WSE was different from that of the FSE as it decreased blood glucose levels without increasing insulin secretion and also lowered glycoalbumin and fructosamine levels in the DM rats. Therefore, the WSE have potential as functional food components with the hypoglycemic effect.
Taniguchi
Hironobu
H
Department of Nutrition, Food Science and Culinary Arts, Toita Women's College, 2-21 17 Shiba, Minato-ku, Tokyo 105-0014, Japan.
Muroi
Rieko
R
Kobayashi-Hattori
Kazuo
K
Uda
Yasushi
Y
Oishi
Yuichi
Y
Takita
Toshichika
T
D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D001786:Blood Glucose / Q000187:drug effects; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D004032:Diet / Q000379:methods; D019270:Fructosamine / Q000097:blood; D017127:Glycation End Products, Advanced; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D007657:Ketone Bodies / Q000097:blood; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000494:pharmacology*; D031224:Raphanus / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D036226:Seedlings; D012709:Serum Albumin / Q000187:drug effects; D013311:Streptozocin; D013997:Time Factors; D000093423:Glycated Serum Albumin
D015415:Biomarkers; D001786:Blood Glucose; D017127:Glycation End Products, Advanced; D007004:Hypoglycemic Agents; D007328:Insulin; D007657:Ketone Bodies; D008055:Lipids; D010936:Plant Extracts; D012709:Serum Albumin; D019270:Fructosamine; D013311:Streptozocin; D000093423:Glycated Serum Albumin