Centrosomal pre-integration latency of HIV-1 in quiescent cells.
Human immunodeficiency virus type 1 (HIV-1) efficiently replicates in dividing and non-dividing cells. However, HIV-1 infection is blocked at an early post-entry step in quiescent CD4+ T cells in vitro. The molecular basis of this restriction is still poorly understood. Here, we show that in quiescent cells, incoming HIV-1 sub-viral complexes concentrate and stably reside at the centrosome for several weeks. Upon cell activation, viral replication resumes leading to viral gene expression. Thus, HIV-1 can persist in quiescent cells as a stable, centrosome-associated, pre-integration intermediate.
Zamborlini
Alessia
A
CNRS UMR7151, Université Paris 7, Hôpital Saint-Louis, Paris, France. alessia.zamborlini@univ-paris-diderot.fr
Lehmann-Che
Jacqueline
J
Clave
Emmanuel
E
Giron
Marie-Lou
ML
Tobaly-Tapiero
Joëlle
J
Roingeard
Philippe
P
Emiliani
Stéphane
S
Toubert
Antoine
A
de Thé
Hugues
H
Saïb
Ali
A
D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D002460:Cell Line; D002478:Cells, Cultured; D018385:Centrosome / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D016662:Virus Integration / Q000502:physiology; D017735:Virus Latency / Q000502:physiology*; D014779:Virus Replication / Q000502:physiology*