Metabolic and genetic profiling of clinical O157 and non-O157 Shiga-toxin-producing Escherichia coli.
A collection of clinical Shiga-toxin-producing Escherichia coli (STEC) strains, mainly belonging to serotypes O26, O103, O111, O145 and O157, was characterised by a polyphasic approach including molecular serotyping, PCR-based detection of virulence factors (stx1, stx2, eae, EHEC-hlyA, saa, katP, espP), carbohydrate fermentation profiles using API50 tests and random amplification of polymorphic DNA (RAPD) fingerprinting. An RAPD protocol based on the combination of 2 primers resulted in sufficiently complex patterns enabling discrimination to the serotype level. Moreover, carbohydrate fermentation profiles obtained after evaluating up to 50 different carbohydrates led to separation of different STEC serotypes. Virulence typing results confirm the association of Shiga toxins and intimin subtypes with specific serotypes and clinical diagnosis. Clinical diagnosis of strains did not correlate with either RAPD profiles or carbohydrate fermentation patterns.
Possé
Björn
B
bjorn.posse@ugent.beUniversity of Ghent, Faculty of Veterinary Medicine, Department of Veterinary Public Health and Food Safety, Laboratory of Hygiene and Technology, Salisburylaan 133, 9820 Merelbeke, Belgium.
De Zutter
Lieven
L
Heyndrickx
Marc
M
Herman
Lieve
L
D018829:Adhesins, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D050260:Carbohydrate Metabolism; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D004927:Escherichia coli Infections / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics; D006801:Humans; D019081:O Antigens / Q000032:analysis; D010544:Peroxidases / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D012697:Serine Endopeptidases / Q000235:genetics; D012703:Serotyping; D022622:Shiga Toxin 1 / Q000235:genetics; D022641:Shiga Toxin 2 / Q000235:genetics; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D037521:Virulence Factors / Q000235:genetics
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D019081:O Antigens; C439650:SAA protein, E coli; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D037521:Virulence Factors; C073819:eaeA protein, E coli; D010544:Peroxidases; C081610:catalase-peroxidase, bacteria; C107189:EspP protein, E coli; D012697:Serine Endopeptidases