A two-dimensional proteome reference map of Herbaspirillum seropedicae proteins.
Herbaspirillum seropedicae is an endophytic diazotroph associated with economically important crops such as rice, sugarcane, and wheat. Here, we present a 2-D reference map for H. seropedicae. Using MALDI-TOF-MS we identified 205 spots representing 173 different proteins with a calculated average of 1.18 proteins/gene. Seventeen hypothetical or conserved hypothetical ORFs were shown to code for true gene products. These data will support the genome annotation process and provide a basis on which to undertake comparative proteomic studies.
Chaves
Daniela Fojo Seixas
DF
Departamento de Bioquímica, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Ferrer
Pércio Pereira
PP
de Souza
Emanuel Maltempi
EM
Gruz
Leonardo Magalhães
LM
Monteiro
Rose Adele
RA
de Oliveira Pedrosa
Fábio
F
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D042803:Herbaspirillum / Q000378:metabolism*; D020543:Proteome / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism
D001426:Bacterial Proteins; D020543:Proteome