Cytomegaloviral proteins pUL50 and pUL53 are associated with the nuclear lamina and interact with cellular protein kinase C.
Human cytomegalovirus-encoded pUL50 and pUL53 belong to a group of conserved herpesviral nuclear proteins. This study describes: (i) the co-localization of pUL50 with components of the nuclear lamina such as lamins A/C and lamin B receptor by double immunofluorescent staining, (ii) a strong pUL50-mediated relocalization of pUL53 from a diffuse nuclear pattern towards a nuclear rim localization, (iii) a direct interaction between pUL50 and pUL53, as well as between pUL50 and protein kinase C (PKC), shown by yeast two-hybrid and co-immunoprecipitation analyses, (iv) in vitro phosphorylation of pUL50, which is highly suggestive of PKC activity, and finally (v) partial relocalization of PKC by pUL50/pUL53 from its main cytoplasmic localization to a marked nuclear lamina accumulation. These data suggest a role for pUL50 and pUL53 in the recruitment of PKC, an event that is considered to be important for cytomegalovirus-induced distortion of the nuclear lamina.
Milbradt
Jens
J
Virological Institute of the University Hospital Erlangen, Clinical and Molecular Virology, University of Erlangen-Nuremberg, 91054 Erlangen, Germany.
Auerochs
Sabrina
S
Virological Institute of the University Hospital Erlangen, Clinical and Molecular Virology, University of Erlangen-Nuremberg, 91054 Erlangen, Germany.
Marschall
Manfred
M
Virological Institute of the University Hospital Erlangen, Clinical and Molecular Virology, University of Erlangen-Nuremberg, 91054 Erlangen, Germany.
D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism* / Q000821:virology; D003587:Cytomegalovirus / Q000378:metabolism* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D009685:Nuclear Envelope / Q000821:virology*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
D011994:Recombinant Proteins; D014764:Viral Proteins; C523758:pUL50 protein, cytomegalovirus; C523759:pUL53 protein, cytomegalovirus; D011493:Protein Kinase C