title: Effects of isoflavones and soybeans fermented with Bacillus subtilis on lipopolysaccharide-induced production of tumor necrosis factor-alpha and fibrinolysis in vivo.
abstract: The effects of isoflavones and of a derivative of soybeans fermented with Bacillus subtilis, designated Nattoesse, on the lipopolysaccharide (LPS)-induced production of tumor necrosis factor-alpha (TNF-alpha) and fibrinolysis were investigated in vivo. The dietary supplement Nattoesse contains several isoflavones. Therefore, we examined the effects of individual isoflavones (daidzein, daidzin, genistein, and genistin) on the LPS-induced production of TNF-alpha. Intraperitoneal injections of daidzein, daidzin, and genistin (but not of genistein before a challenge with LPS) resulted in significant depression of serum levels of TNF-alpha in mice. Daidzein had the strongest activity in this assay. Oral administration of daidzein to mice also had a significant suppressive effect, as compared with that of the Citrus flavanone naringin. In galactosamine-sensitized mice, by contrast, the suppression of LPS-induced lethal shock by daidzein was very weak. Nattoesse did not inhibit the production of TNF-alpha nor did it prevent lethal shock. However, oral administration of Nattoesse to mice significantly suppressed LPS-induced increases in scores of the fibrin degradation product, and the effect was both dose- and time-dependent. Thus, it appears that Nattoesse has fibrinolytic activity during LPS-induced circulatory failure.
authors.affiliation: Department of Biosciences, School of Science and Graduate School of Fundamental Life Science, Kitasato University, Sagamihara, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Hasumuma
authors.forename: Kiichiro
authors.initials: K
authors.lastname: Kawaguchi
authors.forename: Sei-ichi
authors.initials: S
authors.lastname: Kikuchi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kumazawa
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001412:Bacillus subtilis / Q000378:metabolism*; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology; D005260:Female; D005285:Fermentation; D005340:Fibrinogen / Q000096:biosynthesis; D005342:Fibrinolysis / Q000187:drug effects*; D044950:Flavanones / Q000494:pharmacology; D007202:Indicators and Reagents; D007529:Isoflavones / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D010976:Platelet Count; D011517:Prothrombin Time; D013025:Glycine max / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D000975:Antioxidants; D004968:Estrogens, Non-Steroidal; D044950:Flavanones; D007202:Indicators and Reagents; D007529:Isoflavones; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C004742:daidzein; D005340:Fibrinogen; C005274:naringin