title: Physical exercise-associated gene expression signatures in peripheral blood.
abstract: OBJECTIVE
  To assess response to physical stress, gene expression profiles in peripheral blood cells were analyzed using an original microarray carrying 1467 stress-responsive complementary DNA probes.
  
  
  METHODS
  Gene expression was analyzed at 4, 24, and 48 hours after exercising on a cycle ergometer at 60% VO2 max for 1 hour (aerobic exercise) or until exhausted (exhaustive exercise).
  
  
  METHODS
  Institute of Health Biosciences, University of Tokushima Graduate School.
  
  
  METHODS
  Twelve healthy male students of the postgraduate or undergraduate school.
  
  
  METHODS
  The volunteers performed the aerobic or exhaustive exercise on a cycle ergometer.
  
  
  METHODS
  Detection of aerobic exercise-responsive or exhaustive exercise-responsive genes in peripheral blood cells.
  
  
  RESULTS
  Aerobic and exhaustive exercise transiently changed the expression of 21 and 16 genes, respectively, with the peak at 4 hours. Only 2 genes significantly responded to both types of exercise. Exhaustive but not aerobic exercise produced a secondary response with significantly altered expression of 14 genes at 24 hours. Five of those genes encode receptors for neurotransmitters (HTR1A, CHRNB2, GABRB3, GABRG3, and LOC51289).
  
  
  CONCLUSIONS
  The behavior of the individual genes shown here may be informative to objectively assess acute physical stress and exhaustion-associated responses.
authors.affiliation: Department of Stress Science, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kawai
authors.forename: Kyoko
authors.initials: K
authors.lastname: Morita
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Masuda
authors.forename: Kensei
authors.initials: K
authors.lastname: Nishida
authors.forename: Atsuo
authors.initials: A
authors.lastname: Sekiyama
authors.forename: Shigetada
authors.initials: S
authors.lastname: Teshima-Kondo
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakaya
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ohta
authors.forename: Toshiro
authors.initials: T
authors.lastname: Saito
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Rokutan
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics* / Q000502:physiology; D000328:Adult; D001773:Blood Cells; D016552:Ergometry; D015444:Exercise / Q000502:physiology*; D015870:Gene Expression; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011446:Prospective Studies; D012333:RNA, Messenger; D013997:Time Factors
chemical_list: D012333:RNA, Messenger