title: The Chitinase A from the baculovirus AcMNPV enhances resistance to both fungi and herbivorous pests in tobacco.
abstract: Biotechnology has allowed the development of novel strategies to obtain plants that are more resistant to pests, fungal pathogens and other agents of biotic stress. The obvious advantages of having genotypes with multiple beneficial traits have recently fostered the development of gene pyramiding strategies, but less attention has been given to the study of genes that can increase resistance to different types of harmful organisms. Here we report that a recombinant Chitinase A protein of the Autographa californica nuclear polyhedrosis virus (AcMNPV) has both antifungal and insecticide properties in vitro. Transgenic tobacco plants expressing an active ChiA protein showed reduced damages caused by fungal pathogens and lepidopteran larvae, while did not have an effect on aphid populations. To our knowledge, this is the first report on the characterisation and expression in plants of a single gene that increases resistance against herbivorous pests and fungal pathogens and not affecting non-target insects. The implications and the potential of the ChiA gene for plant molecular breeding and biotechnology are discussed.
authors.affiliation: Dipartimento di Scienze del Suolo, della Pianta, dell'Ambiente e delle Produzioni Animali, Università degli Studi di Napoli Federico II, Via Università 100, Portici, Naples, Italy.
authors.forename: Giandomenico
authors.initials: G
authors.lastname: Corrado
authors.forename: Stefania
authors.initials: S
authors.lastname: Arciello
authors.forename: Paolo
authors.initials: P
authors.lastname: Fanti
authors.forename: Luisa
authors.initials: L
authors.lastname: Fiandra
authors.forename: Antonio
authors.initials: A
authors.lastname: Garonna
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Digilio
authors.forename: Matteo
authors.initials: M
authors.lastname: Lorito
authors.forename: Barbara
authors.initials: B
authors.lastname: Giordana
authors.forename: Francesco
authors.initials: F
authors.lastname: Pennacchio
authors.forename: Rosa
authors.initials: R
authors.lastname: Rao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000382:microbiology; D016367:Baculoviridae / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D002688:Chitinases / Q000235:genetics / Q000378:metabolism*; D005658:Fungi / Q000235:genetics / Q000472:pathogenicity*; D015971:Gene Expression Regulation, Enzymologic; D054884:Host-Pathogen Interactions; D007814:Larva / Q000235:genetics / Q000472:pathogenicity; D007915:Lepidoptera / Q000235:genetics / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000201:enzymology* / Q000235:genetics; D010572:Pest Control, Biological; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology*
chemical_list: D011994:Recombinant Proteins; D002688:Chitinases