[Effects of total saponins of Panax japonicus on human leukemic HL-60 cells].
OBJECTIVE
To study the effects of total saponins of Panax japonicus (TSPJ) on human leukemic HL-60 cells.


METHODS
Human leukemic HL-60 cells were cultured in vitro. The cancer cell vigor was detected by using cell counting kit-8. Nitroblue tetrazolium (NBT) was used for measuring cell reduction. The cell cycle and the expression of differentiation antigen CD11b were detected by flow cytometry.


RESULTS
Compared with the negative control group, TSPJ in different concentrations could decrease the vigor of HL-60 cells and the number of cells in S phase and up-regulate the CD11b expression, while the numbers of NBT positive cells and cells in G(0)/G(1) phase in the different concentrations of TSPJ-treated groups were increased.


CONCLUSIONS
TSPJ can inhibit the HL-60 cell growth in vitro. Its mechanism may be related to inhibiting proliferation and inducing cell differentiation and cycle arrest.
Yuan
Ding
D
Medical College, Three Gorges University, Yichang, Hubei Province 443002, China.
Zuo
Rui
R
Zhang
Chang-cheng
CC
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D039481:CD11b Antigen / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D009580:Nitroblue Tetrazolium / Q000032:analysis; D005894:Panax / Q000737:chemistry*; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D039481:CD11b Antigen; D012503:Saponins; D009580:Nitroblue Tetrazolium