title: [Distribution of hepatitis B virus genotypes in patients with chronic hepatitis B virus infection among 12 cities in China].
abstract: OBJECTIVE
  To investigate the distribution of hepatitis B virus (HBV) genotypes in patients with chronic HBV infection among 11 cities of China.
  
  
  METHODS
  A total of 1214 serum samples from patients with chronic HBV infection were collected in 11 cities of China, including Beijing, Qingyuan, Shenzhen, Shijiazhuang, Hanchuan, Nanjing, Changchun, Liaocheng, Jinan, Ningbo and Wenzhou. Genotypes of the 1214 HBV strains were identified by PCR method with type specific primers. Parts of the results were confirmed by direct sequencing analysis of PCR products.
  
  
  RESULTS
  Among the 1214 patients with chronic HBV infection, 0.7% (9/1214)were genotype A, 28.4% (345/1214)genotype B, 58.4% (709/1214) genotype C, and 12.4% (151/1214) genotype B and genotype C mixed infection. No other genotypes were found. Genotype C was predominant in the northern part of China, such as Changchun, Beijing, Shijiazhuang,while genotype B was more commonly seen in south of China. 71.4% (20/28) for patients from Qingyuan and 63.6% (70/110) from Shenzhen were infected with genotype B.
  
  
  CONCLUSIONS
  HBV genotypes had distinct geographic distribution. Genotype B and C the predominant strains in patients with chronic HBV infection in China. Genotype C was predominantly identified in the northern part of China versus genotype B the south.
authors.affiliation: Department of Microbiology, Peking University Health Science Center, Beijing 100083, China.
authors.forename: Jun-wei
authors.initials: JW
authors.lastname: Gao
authors.forename: Ya-juan
authors.initials: YJ
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Zhuang
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.forename: Qing-ming
authors.initials: QM
authors.lastname: Dong
authors.forename: Ya-jie
authors.initials: YJ
authors.lastname: Chen
authors.forename: Jun-qi
authors.initials: JQ
authors.lastname: Niu
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Ma
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Bao-an
authors.initials: BA
authors.lastname: Zhao
authors.forename: Jin-qun
authors.initials: JQ
authors.lastname: Zhong
mesh_terms: D002681:China / Q000453:epidemiology; D005838:Genotype; D005843:Geography; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000453:epidemiology; D006801:Humans; D016133:Polymerase Chain Reaction