Differential effects of soybean and fenugreek extracts on the growth of MCF-7 cells.
The effect of aqueous and ethanol extracts of soybean and fenugreek on the growth of MCF-7 cells, an estrogen receptor positive breast cancer cell line, has been examined in this study. Soybean is well known for the presence of phytoestrogens and fenugreek is reported to have medicinal use including anticancer properties. In a dose dependent manner soybean aqueous and ethanol extract promoted the growth and DNA synthesis in MCF-7 cells. On the contrary ethanol extract of fenugreek decreased the cell viability and induced early apoptotic changes such as flipping of phosphatidylserine and decrease of mitochondrial membrane potential. Degradation of cellular DNA into fragments comprising multiples of approximately 180-200 base pair was also observed. Cell cycle analysis by flow cytometry showed the presence of a subG1 apoptotic population which was more prominent at higher concentrations along with cell cycle arrest at G2/M phase. Our experiments show that while the soybean extract acts as a promoter of MCF-7 cell growth, the fenugreek extract induces apoptosis.
Sebastian
K S
KS
Division of Molecular Endocrinology, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala, India.
Thampan
Raghava Varman
RV
D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D004273:DNA, Neoplasm / Q000187:drug effects; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D010718:Phosphatidylserines / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry*; D029910:Trigonella / Q000737:chemistry*
D004273:DNA, Neoplasm; D010718:Phosphatidylserines; D010936:Plant Extracts