title: Centrosomal pre-integration latency of HIV-1 in quiescent cells.
abstract: Human immunodeficiency virus type 1 (HIV-1) efficiently replicates in dividing and non-dividing cells. However, HIV-1 infection is blocked at an early post-entry step in quiescent CD4+ T cells in vitro. The molecular basis of this restriction is still poorly understood. Here, we show that in quiescent cells, incoming HIV-1 sub-viral complexes concentrate and stably reside at the centrosome for several weeks. Upon cell activation, viral replication resumes leading to viral gene expression. Thus, HIV-1 can persist in quiescent cells as a stable, centrosome-associated, pre-integration intermediate.
authors.affiliation: CNRS UMR7151, Université Paris 7, Hôpital Saint-Louis, Paris, France. alessia.zamborlini@univ-paris-diderot.fr
authors.forename: Alessia
authors.initials: A
authors.lastname: Zamborlini
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Lehmann-Che
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Clave
authors.forename: Marie-Lou
authors.initials: ML
authors.lastname: Giron
authors.forename: Joëlle
authors.initials: J
authors.lastname: Tobaly-Tapiero
authors.forename: Philippe
authors.initials: P
authors.lastname: Roingeard
authors.forename: Stéphane
authors.initials: S
authors.lastname: Emiliani
authors.forename: Antoine
authors.initials: A
authors.lastname: Toubert
authors.forename: Hugues
authors.initials: H
authors.lastname: de Thé
authors.forename: Ali
authors.initials: A
authors.lastname: Saïb
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D002460:Cell Line; D002478:Cells, Cultured; D018385:Centrosome / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D016662:Virus Integration / Q000502:physiology; D017735:Virus Latency / Q000502:physiology*; D014779:Virus Replication / Q000502:physiology*