High-throughput in vivo analysis of gene expression in Caenorhabditis elegans.
Using DNA sequences 5' to open reading frames, we have constructed green fluorescent protein (GFP) fusions and generated spatial and temporal tissue expression profiles for 1,886 specific genes in the nematode Caenorhabditis elegans. This effort encompasses about 10% of all genes identified in this organism. GFP-expressing wild-type animals were analyzed at each stage of development from embryo to adult. We have identified 5' DNA regions regulating expression at all developmental stages and in 38 different cell and tissue types in this organism. Among the regulatory regions identified are sequences that regulate expression in all cells, in specific tissues, in combinations of tissues, and in single cells. Most of the genes we have examined in C. elegans have human orthologs. All the images and expression pattern data generated by this project are available at WormAtlas (http://gfpweb.aecom.yu.edu/index) and through WormBase (http://www.wormbase.org).
Hunt-Newbury
Rebecca
R
Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada.
Viveiros
Ryan
R
Johnsen
Robert
R
Mah
Allan
A
Anastas
Dina
D
Fang
Lily
L
Halfnight
Erin
E
Lee
David
D
Lin
John
J
Lorch
Adam
A
McKay
Sheldon
S
Okada
H Mark
HM
Pan
Jie
J
Schulz
Ana K
AK
Tu
Domena
D
Wong
Kim
K
Zhao
Z
Z
Alexeyenko
Andrey
A
Burglin
Thomas
T
Sonnhammer
Eric
E
Schnabel
Ralf
R
Jones
Steven J
SJ
Marra
Marco A
MA
Baillie
David L
DL
Moerman
Donald G
DG
D000367:Age Factors; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins; D011993:Recombinant Fusion Proteins; D014018:Tissue Distribution
D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins