Envelope protein Us9 is required for the anterograde transport of bovine herpesvirus type 1 from trigeminal ganglia to nose and eye upon reactivation.
In this study, the authors examined the role of bovine herpesvirus type 1 (BHV-1) Us9 in the anterograde transport of the virus from trigeminal ganglia (TG) to nose and eye upon reactivation from latency. During primary infection, both BHV-1 Us9-deleted and BHV-1 Us9-rescued viruses replicated efficiently in the nasal and ocular epithelium. However, upon reactivation from latency, only the BHV-1 Us9-rescued virus could be isolated in the nasal and ocular shedding. By real-time polymerase chain reaction, comparable DNA copy numbers were detected in the TGs during latency and reactivation for both the viruses. Therefore, Us9 is essential for reactivation of the virus in the TG and anterograde axonal transport from TG to nose and eye.
Butchi
N B
NB
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA.
Jones
C
C
Perez
S
S
Doster
A
A
Chowdhury
S I
SI
D000818:Animals; D002417:Cattle; D004279:DNA, Viral / Q000032:analysis; D005123:Eye / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D007241:Infectious Bovine Rhinotracheitis / Q000821:virology*; D009666:Nose / Q000821:virology; D012668:Trigeminal Ganglion / Q000821:virology*; D014759:Viral Envelope Proteins / Q000502:physiology*; D014774:Virulence; D017735:Virus Latency
D004279:DNA, Viral; C524555:US9 protein, bovine herpesvirus 1; D014759:Viral Envelope Proteins