[Nuclear expression of S100A4 is associated with lymph node metastasis in gastric carcinoma].
OBJECTIVE
To investigate the intracellular localization of S100A4 in gastric carcinoma cells and the relationship between S100A4 expression status and lymph node metastasis of gastric carcinoma.


METHODS
Western blotting analysis was performed to locate the expression of S100A4 protein in sub-fraction components of frozen tissues. S100A4 protein expression was also determined by immunohistochemical method in 131 samples of gastric cancer and 20 samples of matched metastatic lymph nodes.


RESULTS
Thirty-two of 131 (24.4%) gastric carcinoma showed positive S100A4 nuclear expression and 50/131 (38.2%) carcinoma showed positive cytoplasmic expression. In 32 samples with positive S100A4 nuclear expression, 30 (93.8%) carcinomas had positive lymph node metastases. S100A4 nuclear expression level was higher in gastric carcinoma with lymph node metastasis (29.1%) than that without lymph node metastasis (7.1%) (P=0.016).


CONCLUSIONS
Nuclear expression of S100A4 is associated with lymph node metastasis of gastric carcinoma.
Zhong
Xi-yao
XY
Department of Gastrointestinal Surgery, Peking University School of Oncology, Beijing Cancer Hospital, Beijing Institute for Cancer Research, Beijing 100036, China.
Zhang
Lian-hai
LH
Jia
Shu-qin
SQ
Shi
Tao
T
DU
Hong
H
Hu
Ying
Y
Zhang
Gui-guo
GG
Lu
Ai-ping
AP
Li
Ji-you
JY
Ji
Jia-fu
JF
D002467:Cell Nucleus / Q000378:metabolism*; D006801:Humans; D008207:Lymphatic Metastasis; D009367:Neoplasm Staging; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology*
D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C077591:S100A4 protein, human