Babesia gibsoni: identification of an immunodominant, interspersed repeat antigen.
In this report, an immunodominant antigen called BgIRA from Babesia gibsoni is identified and described. A highly repetitive antigen was screened from a cDNA library. The genomic BgIRA gene exists as single cope gene and contains 10 introns. BgIRA plays a dominant role in the immune response in dogs infected with B. gibsoni. The specificity and sensitivity of the rBgIRA in an ELISA indicated that this antigen might be useful in a diagnostic test.
Jia
Honglin
H
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
Terkawi
M Alaa
MA
Aboge
Gabriel Oluga
GO
Goo
Youn-Kyoung
YK
Zhou
Jinlin
J
Lee
Eung-Goo
EG
Nishikawa
Yoshifumi
Y
Igarashi
Ikuo
I
Fujisaki
Kozo
K
Xuan
Xuenan
X
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D001403:Babesia / Q000235:genetics / Q000276:immunology*; D001404:Babesiosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D004283:Dog Diseases / Q000175:diagnosis / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015723:Gene Library; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D012680:Sensitivity and Specificity; D013047:Specific Pathogen-Free Organisms
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016056:Immunodominant Epitopes