Neuroprotection and free radical scavenging effects of Osmanthus fragrans.
The ethanol extract of dried flowers Osmanthus fragrans (OFE) was assessed for free radical scavenging effects measured by the bleaching of the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical, scavenging of the hydroxyl anion, investigation of the ferric reducing/antioxidant power (FRAP) and lipid-peroxidation inhibition in rat tissues. OFE contained a high amount of total flavonoid and polyphenol. OFE presented the effects in the metal reducing power, FRAP assay with IC(50) values of 0.23 microg/ml, and 7.74 microg/ml, respectively. OFE presented similar activities toward the DPPH and hydroxyl anion scavenging ability with IC(50) values of 10 microg/ml. OFE with IC(50 )values between 46 and 97 microg/ml inhibited lipid peroxidation initiated by ferrous chloride in rat brain, liver, heart and kidney mitochodrias. Moreover, the neuroprotective activity of OFE was investigated under different insults (glutamate, arachidonic acid, and 6-hydroxydopamine) in Wistar rat primary cortical neurons. OFE with EC(50 )values between 66 and 165 microg/ml attenuated the neurotoxicity on MTT and LDH assays. In addition, the AKT protein expression of excitotoxicity and oxidative stress was displayed by western blotting analysis. OFE could up-regulate the glutamate and 6-OHDA decreased AKT expression. This is the first demonstration of the neuroprotective, free radical scavenging and anti-oxidative effects of O. fragrans.
Lee
Hsin-Hsueh
HH
Department of Pharmacognosy, School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan.
Lin
Chi-Ting
CT
Yang
Ling-Ling
LL
D000818:Animals; D016718:Arachidonic Acid / Q000633:toxicity; D005260:Female; D005296:Ferrous Compounds / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology*; D018698:Glutamic Acid / Q000494:pharmacology / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D009474:Neurons / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D029592:Oleaceae / Q000737:chemistry*; D016627:Oxidopamine / Q000633:toxicity; D010936:Plant Extracts / Q000737:chemistry; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D005296:Ferrous Compounds; D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D010936:Plant Extracts; D016718:Arachidonic Acid; D018698:Glutamic Acid; D016627:Oxidopamine; D051057:Proto-Oncogene Proteins c-akt; C029451:ferrous chloride