title: Use of fluorescent quantum dot bioconjugates for cellular imaging of immune cells, cell organelle labeling, and nanomedicine: surface modification regulates biological function, including cytotoxicity.
abstract: With the development of nanotechnology, nanoscale products that are smaller than several hundred nanometers have been applied to all areas of science and technology. Nanoscale products, including carbon nanotubes, fullerene derivatives, and nanocrystal quantum dots (QDs), are wide spread as novel tools in various fields, not only in materials engineering, electronics, plastics, and the automobile and aerospace industries, but also in molecular biology and medicine. At present, QDs have been widely used in biological and medical studies because of their superior photoemission and photostability. Although the physical and chemical properties of QDs have been circumstantially investigated, little is known about any harmful effects of QDs on human health. Here we report on the toxicity and biological behavior of QDs in vitro and in vivo. The toxicity of the core constituent chemicals such as cadmium and selenium has been identified. Recently, the surface molecules surrounding QDs have been intensively investigated. Accumulating evidence that toxic surface-covering molecules showed their cytotoxicity and biomolecules conjugated with QDs maintained their biological effects indicates that at least the biological properties of QDs are attributable to the QD-capping material rather than to the core metalloid complex itself.
authors.affiliation: International Clinical Research Center, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Hoshino
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Manabe
authors.forename: Kouki
authors.initials: K
authors.lastname: Fujioka
authors.forename: Kazuo
authors.initials: K
authors.lastname: Suzuki
authors.forename: Masato
authors.initials: M
authors.lastname: Yasuhara
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects*; D002522:Chlorocebus aethiops; D016207:Cytokines / Q000187:drug effects; D006801:Humans; D007962:Leukocytes / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D015336:Molecular Probe Techniques; D050997:Nanomedicine / Q000379:methods; D053758:Nanoparticles / Q000633:toxicity; D015388:Organelles; D045663:Quantum Dots; D013194:Staining and Labeling / Q000379:methods*; D014709:Vero Cells / Q000166:cytology / Q000187:drug effects
chemical_list: D016207:Cytokines