Mover is a novel vertebrate-specific presynaptic protein with differential distribution at subsets of CNS synapses.
Presynaptic nerve terminals contain scaffolding proteins that orchestrate neurotransmitter release at active zones. Here we describe mover, a yet unknown non-transmembrane protein that is targeted to presynaptic terminals when overexpressed in cultured neurons. Confocal immunomicroscopy revealed that mover colocalizes with presynaptic markers in the calyx of Held. In the hippocampus, mover localizes to mossy fibre terminals, but is absent from inhibitory nerve terminals. By contrast, mover localizes to inhibitory terminals throughout the cerebellar cortex. Our results suggest that mover may act in concert with generally expressed scaffolding proteins in distinct sets of presynaptic terminals.
Kremer
Thomas
T
Institute for Anatomy and Cell Biology, University of Heidelberg, Im Neuenheimer Feld 307, D-69120 Heidelberg, Germany.
Kempf
Christian
C
Wittenmayer
Nina
N
Nawrotzki
Ralph
R
Kuner
Thomas
T
Kirsch
Joachim
J
Dresbach
Thomas
T
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002490:Central Nervous System / Q000378:metabolism*; D017124:Conserved Sequence; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D051381:Rats; D016415:Sequence Alignment; D013569:Synapses / Q000378:metabolism*; D046509:Tissue Culture Techniques
D009419:Nerve Tissue Proteins; C524252:Tprg1l protein, rat