A fast CE method for the achiral separation of methadone and its major metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine and 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline.
The utilization of dynamic doubly coated capillaries for a fast separation of methadone and its two major metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline (EMDP) was investigated. The coated capillaries were prepared using a polycation of poly(diallyldimethylammonium chloride) and a polyanion of dextran sulfate. A fast achiral separation was developed using the coated capillaries with a BGE of 100 mM phosphate buffer at pH 2.6. Complete achiral separation of methadone, EDDP and EMDP was achieved, with migration times of approximately 4 min. The method offers considerable advantages with respect to BGE simplicity and analysis time compared to previously published CE methods for methadone and its related analytes.
Kelly
Tamsin
T
Centre for Forensic Science, Faculty of Science, University of Technology, Sydney, NSW, Australia.
Doble
Philip
P
Dawson
Michael
M
D002138:Calibration; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D016264:Dextran Sulfate / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D053593:Forensic Toxicology / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008691:Methadone / Q000032:analysis* / Q000378:metabolism; D010710:Phosphates; D011095:Polyethylenes / Q000737:chemistry; D011759:Pyrrolidines / Q000032:analysis* / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
C103254:2-ethyl-5-methyl-3,3-diphenylpyrroline; D020099:Coated Materials, Biocompatible; D010710:Phosphates; D011095:Polyethylenes; D011759:Pyrrolidines; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D016264:Dextran Sulfate; C018279:sodium phosphate; D008691:Methadone; C010724:2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine