title: Initial experiences using non-cultured autologous keratinocyte suspension for burn wound closure.
abstract: Early complete wound closure and thus reduction of excessive scar formation still represent a major clinical challenge in severely burned patients. A novel concept to cover large burn wounds consists of the application of non-cultured epithelial cell suspension within the first days. Herein, we report our experiences with three patients treated with CellSpray XP. According to the amount of cell suspension required, a skin biopsy was harvested and then processed in an external laboratory. Two days later the suspension containing autologous non-cultured keratinocytes was applied using an aerosol system. All wounds healed rapidly and virtually no signs of hypertrophic scarring were observed 6 months later.
authors.affiliation: Division of Plastic and Reconstructive Surgery, Burns Unit, University Hospital of Zurich, Zurich, Switzerland. claire.zweifel@usz.ch
authors.forename: C J
authors.initials: CJ
authors.lastname: Zweifel
authors.forename: C
authors.initials: C
authors.lastname: Contaldo
authors.forename: C
authors.initials: C
authors.lastname: Köhler
authors.forename: A
authors.initials: A
authors.lastname: Jandali
authors.forename: W
authors.initials: W
authors.lastname: Künzi
authors.forename: P
authors.initials: P
authors.lastname: Giovanoli
mesh_terms: D000328:Adult; D000336:Aerosols; D001706:Biopsy; D002056:Burns / Q000473:pathology / Q000628:therapy*; D017439:Cicatrix, Hypertrophic / Q000517:prevention & control; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D008297:Male; D012867:Skin / Q000473:pathology; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D014945:Wound Healing
chemical_list: D000336:Aerosols