A rapid CZE method for the analysis of benzodiazepines in spiked beverages.
A rapid CZE method was developed for the simultaneous determination of nine benzodiazepines in spiked beverages (nitrazepam oxazepam, alprazolam, flunitrazepam, temazepam, diazepam, 7-aminoflunitrazepam, 7-aminonitrazepam and 7-aminoclonazepam). The method employed a double-coated capillary coated with poly(diallyldimethylammonium chloride) and then dextran sulphate. The BGE conditions were 100 mM ammonium phosphate buffer, pH 2.5, which gave baseline resolution between consecutive peaks and a run time of less than 6.5 min. This method offers improvements in both resolution and run time, compared to those attained under analogous conditions with an uncoated capillary. The validated method was successfully applied to beverages that had been spiked with benzodiazepines at concentrations simulating prescription tablets. No sample pretreatment was required to quantify five benzodiazepines in Coca-Cola, orange juice, beer, bourbon and Bacardi. The exception was white wine, where the complex sample matrix did not enable the accurate quantification of nitrazepam.
Webb
Rebecca
R
Centre for Forensic Science, Department of Chemistry, Materials and Forensic Science, University of Technology, Sydney, NSW, Australia.
Doble
Philip
P
Dawson
Michael
M
D000434:Alcoholic Beverages / Q000032:analysis* / Q000145:classification; D014151:Anti-Anxiety Agents / Q000032:analysis* / Q000737:chemistry; D001569:Benzodiazepines / Q000032:analysis* / Q000737:chemistry; D001628:Beverages / Q000032:analysis* / Q000145:classification; D002138:Calibration; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D016264:Dextran Sulfate / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D053593:Forensic Toxicology / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010710:Phosphates; D011095:Polyethylenes / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D014151:Anti-Anxiety Agents; D020099:Coated Materials, Biocompatible; D010710:Phosphates; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C024788:ammonium phosphate; D001569:Benzodiazepines; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D016264:Dextran Sulfate