Evaluation of the effect of ketoprofen and carprofen on platelet function in dogs studied by PFA-100 point-of-care analyser.
The effect of two nonsteroidal anti-inflammatory drugs (carprofen and ketoprofen) on platelet adhesion and aggregation functions was evaluated by the PFA-100 analyser (Dade-Behring, CA, U.S.A.) using its collagen-adenosine diphosphate (ADP) and collagen-epinephrine (EPI) cartridges. The function of platelets was evaluated in 55 healthy dogs, in 7 dogs treated with ketoprofen and in 31 dogs treated with carprofen in a therapeutic dose for minimum 5 days. The therapeutic doses of carprofen had no effect on the closure time of PFA-100 (which is the marker of platelet function) but ketoprofen caused a significant increase when using collagen-EPI stimulation The closure times for both the healthy (control) and the treated dogs using EPI cartridges were often longer than the upper default cut-off point (300 sec) of the device. The PFA-100 analyser with collagen-ADP cartridges could be a useful tool for veterinary applications including the evaluation of platelet aggregation in dogs treated with NSAIDs. The upper cut-off point of PFA-100 might be extended.
Gaál
T
T
Department of Internal Medicine Faculty of Veterinary Science, Szent István University, H-1078 Budapest, István u. 2, Hungary. Gaal.Tibor@aotk.szie.hu
Halmay
Dóra
D
Kocsis
R
R
Abonyi-Tóth
Z
Z
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D002227:Carbazoles / Q000494:pharmacology*; D004285:Dogs / Q000097:blood*; D005260:Female; D007660:Ketoprofen / Q000494:pharmacology*; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects* / Q000502:physiology; D010979:Platelet Function Tests / Q000295:instrumentation / Q000379:methods / Q000662:veterinary
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002227:Carbazoles; D007660:Ketoprofen; C007005:carprofen