title: Proteomic analysis of somatic embryogenesis in Vitis vinifera.
abstract: Two dimensional gel electrophoresis coupled to mass spectrometry has been used to study the somatic embryogenesis in Vitis vinifera, by comparing embryogenic and non embryogenic calluses of the Thompson seedless cv. More than 1,000 spots were reproducibly resolved in colloidal Coomassie brilliant blue stained gels over a pI nonlinear range of 3-10 in the first dimension and using homogeneous 12.5% polyacrylamide gels in the second dimension. The expression pattern of 35 spots differed significantly between the two samples. These spots were processed by mass spectrometry analysis and the protein identity was assigned by using both the non-redundant protein and EST databases. Several responsive proteins, some already known to be involved in the somatic embryogenesis process while others, for the first time put into relation with this process, have been described. Moreover, they have been subdivided in functional categories, and their putative role is discussed in terms of their relevance in the somatic embryogenesis process.
authors.affiliation: Dipartimento Ambiente-Salute-Sicurezza, Università dell'Insubria, via J.H. Dunant 3, 21100 Varese, Italy. milena.marsoni@uninsubria.it
authors.forename: Milena
authors.initials: M
authors.lastname: Marsoni
authors.forename: Marcella
authors.initials: M
authors.lastname: Bracale
authors.forename: Luca
authors.initials: L
authors.lastname: Espen
authors.forename: Bhakti
authors.initials: B
authors.lastname: Prinsi
authors.forename: Alfredo S
authors.initials: AS
authors.lastname: Negri
authors.forename: Candida
authors.initials: C
authors.lastname: Vannini
mesh_terms: D002853:Chromatography, Liquid; D003583:Cytokinins / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D007210:Indoleacetic Acids / Q000494:pharmacology; D010940:Plant Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D027843:Vitis / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism*
chemical_list: D003583:Cytokinins; D007210:Indoleacetic Acids; D010940:Plant Proteins