Schizosaccharomyces pombe nup97, which Genetically Interacts with mex67, is essential for growth and involved in mRNA export.
We have isolated previously three synthetic lethal mutants in Schizosaccharomyces pombe, which genetically interact with mex67, in order to identify the genes involved in mRNA export. A novel nup97 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMex3. The nup97 gene contains one intron and encodes an 851 amino-acid protein that is similar to nucleoporins, Npp106p in S. pombe and Nic96p in Saccharomyces cerevisiae. The nup97 gene is essential for vegetative growth, and nup97 null mutant harboring pREP41X-Nup97 showed poly(A)+ RNA export defect when expression of nup97 is repressed in the presence of thiamine. These results suggest that nup97 is involved in mRNA export from the nucleus to cytoplasm.
Cho
Hyun Jin
HJ
Deptartment of Biology, Institute of Basic Sciences, Sungshin Women's University, Seoul 136-742, Republic of Korea.
Hwang
Duk Kyung
DK
Jung
Sun Im
SI
Yoon
Jin Ho
JH
D020043:Genes, Essential; D005800:Genes, Fungal / Q000235:genetics* / Q000502:physiology; D005816:Genetic Complementation Test; D008957:Models, Genetic; D009154:Mutation; D034443:RNA Transport; D012331:RNA, Fungal / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D012331:RNA, Fungal; D012333:RNA, Messenger