In vitro effects of calcium fructoborate on fMLP-stimulated human neutrophil granulocytes.
Discovery of naturally occurring boron complexes with organic compounds containing hydroxyl groups, sugars, and polysaccharides, adenosine-5-phosphate, pyridoxine, riboflavin, dehydroascorbic acid, and pyridine nucleotides led to the reassessment of the biochemical role of boron. Boron's anti-inflammatory actions were claimed but not yet demonstrated. This study investigated the effects of calcium fructoborate (CF) on the human polymorphonuclear neutrophils (PMN) that play a central role in the inflammatory response. Our results demonstrated that CF exposure induced a dose-dependent decrease in cell viability. Treatment of PMN cells, for 24 h, with 22,500 microM CF led to a decrease in cell viability by 61.1%, an inhibition of respiratory burst by 92.9% in the case of fMLP-stimulated cells, a diminution of intracellular level of superoxide anion with 59.3%, and a stimulation of superoxide dismutase activity by 72% in unstimulated PMN cells. Altogether, these results suggest the antioxidant and anti-inflammatory properties of CF.
Scorei
R
R
Department of Biochemistry, University of Craiova, 13 A.I. Cuza, Craiova, 200585, Romania. romulus_ion@yahoo.com
Ciubar
Raluca
R
Iancu
Cristina
C
Mitran
Valentina
V
Cimpean
Anisoara
A
Iordachescu
Dana
D
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000502:physiology; D001881:Borates / Q000494:pharmacology*; D002122:Calcium Chloride / Q000494:pharmacology; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D005632:Fructose / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D016897:Respiratory Burst; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001881:Borates; C507177:calcium fructoborate; D013481:Superoxides; D005632:Fructose; D009240:N-Formylmethionine Leucyl-Phenylalanine; C010634:sodium borate; D013482:Superoxide Dismutase; D002122:Calcium Chloride