RCAS1 decidual immunoreactivity in severe pre-eclampsia: immune cell presence and activity.
OBJECTIVE
Pre-eclampsia seems to be related to the disturbance of immune tolerance regulation during pregnancy. Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) decidual level alterations were concomitant with changes in immune cell number and activity in decidua. As decidual immunomodulating activity participates in the development of immune tolerance during pregnancy, we aimed to evaluate the immunoreactivity level of decidual RCAS1 with respect to the presence and activity of immune cells.


METHODS
RCAS1, CD3, CD56, CD69, and CD25 immunoreactivity was assessed by immunohistochemistry in 30 decidual samples derived from patients with severe pre-eclampsia (sPE) and from a healthy control group.


RESULTS
RCAS1 immunoreactivity was statistically significantly higher in decidual tissue samples derived from patients with sPE tissue than in those derived from healthy patients in whom elective cesarean section at term was performed. A statistically significantly lower number of CD56(+) and CD3(+) cells and lower immunoreactivity level of CD69 were found in patients with sPE compared with those from the control group.


CONCLUSIONS
The limited immune cells infiltration in decidua during sPE is associated with increase in RCAS1 decidual level.
Wicherek
Lukasz
L
Department of Gynecology and Oncology of the Jagiellonian University, Krakow, Poland. owicher@cyf-kr.edu.pl
Basta
Pawel
P
Sikora
Jerzy
J
Galazka
Krystyna
K
Rytlewski
Krzysztof
K
Grabiec
Marek
M
Lazar
Agata
A
Kalinka
Jaroslaw
J
D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis*; D017252:CD3 Complex / Q000032:analysis; D019002:CD56 Antigen / Q000032:analysis; D016022:Case-Control Studies; D003656:Decidua / Q000276:immunology*; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D007150:Immunohistochemistry; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis; D037181:Lectins, C-Type; D007958:Leukocyte Count; D011225:Pre-Eclampsia / Q000097:blood / Q000276:immunology*; D011247:Pregnancy; D012720:Severity of Illness Index; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D017252:CD3 Complex; D019002:CD56 Antigen; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D000099192:CD69 Antigens; C120762:EBAG9 protein, human