title: Mutational analysis of ErbB2 intracellular localization.
abstract: In the present study, the sub-cellular localization of ErbB2 and its mutants expressed as GFP-tagged proteins in MCF-7 cells or endogenous ErbB2 in SKBR3 cells was examined. The data presented here demonstrate that the full-length ErbB2 was localized at the cytoplasmic membrane and ErbB2 ICD localized in the nucleus predominantly. The sequence of ErbB2 ICD contains the information supporting its nuclear translocation and cytoplasmic retention. A region (residues 721-970) harboring an arginine triplet is essential for the cytoplasmic trafficking of ErbB2. The results indicate that differential sub-cellular localization of ErbB2 ICD and the full-length ErbB2 is dependent on their structural determinants. The present results give initial clues for further analysis of the mechanism of ErbB2 intracellular localization.
authors.affiliation: Institute of Basic Medical Sciences, Taiping Road 27, Beijing, 100850, People's Republic of China.
authors.forename: Liyong
authors.initials: L
authors.lastname: Chen
authors.forename: Lu
authors.initials: L
authors.lastname: Qian
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Shi
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Song
authors.forename: Guogang
authors.initials: G
authors.lastname: Yuan
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Meiru
authors.initials: M
authors.lastname: Hu
authors.forename: Ming
authors.initials: M
authors.lastname: Yu
authors.forename: Xuemin
authors.initials: X
authors.lastname: Zhang
authors.forename: Beifen
authors.initials: B
authors.lastname: Shen
authors.forename: Ning
authors.initials: N
authors.lastname: Guo
mesh_terms: D000818:Animals; D016466:CHO Cells; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D004252:DNA Mutational Analysis; D006801:Humans; D019913:Nuclear Localization Signals / Q000235:genetics; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D019913:Nuclear Localization Signals; D018719:Erb-b2 Receptor Tyrosine Kinases