title: [Serum values of interleukin-10, gamma-interferon and vitamin A in female adolescents].
abstract: Many studies have demonstrated that vitamin A deficiency (VAD) affects the immunomodulated response mediated by cytokines. However, these studies are controversial. The purpose of the present study was to analyze Interleukin-10, gamma-Interferon and vitamin A serum concentrations in adolescents. Seventy three female, not pregnant adolescents (15.95 +/- 1.10 years old), of a low socioeconomic condition were studied. Serum retinol was determined by HPLC using the Bieri method. International reference standards were considered to define VAD (serum retinol < 20 microg/dL), risk of VAD (20-30 microg/dL) and vitamin A sufficiency (>30 microg/dL). Serum concentrations of Interleukine-10 (IL-10) and gamma-Interferon (gamma-IFN) were detected by an ELISA method (pg/mL). The data were analyzed using the SAS/STAT statistical program; the results were presented as mean +/- Standard deviation and the differences between mean values were analyzed by the ANOVA test. The prevalence of VAD in adolescents was 6.85% (serum retinol <20 microg/dL) and 41.10% adolescents had VAD risk (20-30 microg/dL). Adolescents with VAD showed a significant increase of gamma-IFN serum concentration (p = 0.01). Correlation between serum retinol and gamma-IFN was r = -0.29 (p = 0.01). Adolescents represent a VAD risk group. Low serum levels of retinol were correlated with high levels of gamma-IFN, this cytokine has been associated with chronics inflammatory processes and it can contribute to increase the morbidity and mortality in this population.
authors.affiliation: Laboratorio de Investigación en Malnutrición Infantil y Retardo Mental, Instituto do Invcstigaciones Biológicas, Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela. jyleal@hotmail.com
authors.forename: Jorymar Y
authors.initials: JY
authors.lastname: Leal
authors.forename: Tania
authors.initials: T
authors.lastname: Romero
authors.forename: Pablo
authors.initials: P
authors.lastname: Ortega
authors.forename: Daisy
authors.initials: D
authors.lastname: Amaya
mesh_terms: D000293:Adolescent; D000328:Adult; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D016753:Interleukin-10 / Q000097:blood*; D012016:Reference Values; D012306:Risk; D012494:Sampling Studies; D012959:Socioeconomic Factors; D014687:Venezuela / Q000453:epidemiology; D014801:Vitamin A / Q000097:blood*; D014802:Vitamin A Deficiency / Q000453:epidemiology*
chemical_list: C508609:IL10 protein, human; D014801:Vitamin A; D016753:Interleukin-10; D007371:Interferon-gamma