[Expression of ATP-binding cassette transporter ABCG2 in gastric carcinoma and its significance].
OBJECTIVE
To investigate the expression of ATP-binding cassette transporter (ABCG2) in gastric carcinoma and its clinical significance.


METHODS
Expression of ABCG2 protein was examined by immunohistochemical technique in specimens from 45 gastric carcinoma tissues and 30 surrounding normal tissues. The expression of ABCG2 mRNA was measured by RT-PCR and real-time quantitative PCR in specimens from 30 gastric carcinoma tissues and 30 surrounding normal gastric mucosa respectively.


RESULTS
ABCG2 protein expression was observed in 28 of 45 (62.2%) cases by immunohistochemical analysis, while in 2 of 30 (6.7%) normal tissues (P< 0.05). In ABCG2-positive tumors, adjacent non-neoplastic tissue was similarly analyzed and revealed that ABCG2 was up-regulated in gastric carcinoma. The positive rates of ABCG2 expression in poorly differentiated and/or undifferentiated carcinoma were significantly higher than those in well and/or moderately-differentiated carcinoma (P< 0.05). The mRNA expression of ABCG2 was significantly higher in gastric carcinoma tissue than that in normal gastric mucosa (P< 0.05).


CONCLUSIONS
ABCG2 expression is up-regulated in gastric carcinoma. ABCG2 may be an important factor in the research of gastric cancer stem cell.
Wang
Ning
N
Department of General Surgery, General Hospital of PLA, Beijing 100853, China.
Chen
Lin
L
Wei
Bo
B
Zhao
Xiang-yang
XY
Lu
Wen-jing
WJ
Shen
Li
L
D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D000368:Aged; D019008:Drug Resistance, Neoplasm; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009367:Neoplasm Staging; D014411:Neoplastic Stem Cells; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D055815:Young Adult
C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D009363:Neoplasm Proteins