title: PrrC, a Sco homologue from Rhodobacter sphaeroides, possesses thiol-disulfide oxidoreductase activity.
abstract: PrrC is a Sco homologue in Rhodobacter sphaeroides that is associated with PrrBA, a two-component signal transduction system that induces photosynthesis gene expression in response to a decrease in oxygen tension. Although Sco proteins have been shown to bind copper the observation that they are structurally-related to thioredoxins suggested that they might possess thiol-disulfide oxidoreductase activity. Our results show that PrrC reduces Cu(2+) to Cu(+) and possesses disulfide reductase activity. These results indicate that some bacterial Sco proteins may have biochemical properties that are distinct from those of mitochondrial Sco proteins.
authors.affiliation: Centre for Metals in Biology, School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, Queensland 4072, Australia.
authors.forename: Alison C
authors.initials: AC
authors.lastname: Badrick
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Hamilton
authors.forename: Paul V
authors.initials: PV
authors.lastname: Bernhardt
authors.forename: Christopher E
authors.initials: CE
authors.lastname: Jones
authors.forename: Ulrike
authors.initials: U
authors.lastname: Kappler
authors.forename: Michael P
authors.initials: MP
authors.lastname: Jennings
authors.forename: Alastair G
authors.initials: AG
authors.lastname: McEwan
mesh_terms: D002413:Cations, Divalent / Q000737:chemistry; D003300:Copper / Q000737:chemistry / Q000378:metabolism; D004563:Electrochemistry; D007328:Insulin / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D011485:Protein Binding; D011490:Protein Disulfide Reductase (Glutathione) / Q000235:genetics / Q000378:metabolism*; D012242:Rhodobacter sphaeroides / Q000201:enzymology* / Q000235:genetics; D013053:Spectrophotometry; D013879:Thioredoxins / Q000378:metabolism
chemical_list: D002413:Cations, Divalent; D007328:Insulin; D013879:Thioredoxins; D003300:Copper; D011490:Protein Disulfide Reductase (Glutathione)