title: Porcine plasma ficolin binds and reduces infectivity of porcine reproductive and respiratory syndrome virus (PRRSV) in vitro.
abstract: Ficolins are collagenous lectins that bind N-acetylated glycans and participate in innate immune responses, including phagocytosis and complement activation. Related collagenous lectins such as mannan binding lectin (MBL) and surfactant proteins A and D possess antiviral activity, but this activity has not been demonstrated for ficolins. In these studies, we used purified porcine plasma ficolin alpha and recombinant ficolin alpha to assess their ability to bind and neutralize porcine reproductive and respiratory virus (PRRSV) in various assays. Recombinant ficolin alpha was designed with a C-terminal 6-histidine tag using a pcDNA3.1 expression vector system in CHO K1 cells. Plasma-purified and recombinant ficolin alpha reduced cytopathic effect of PRRSV-infected Marc-145 cells in neutralization assays and inhibited replication of infectious viral particles in a GlcNAc-dependent manner. In vitro replication determined by plaque assay was inhibited in the presence of plasma-purified ficolin alpha and recombinant ficolin. Immunoreactive plasma ficolin alpha and recombinant ficolin alpha also bound PRRSV-coated wells in a GlcNAc-dependent manner. These studies indicate that porcine ficolin can bind and neutralize a common arterivirus that is a major pathogen of swine.
authors.affiliation: Department of Pathobiology, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Natalie D
authors.initials: ND
authors.lastname: Keirstead
authors.forename: Changhee
authors.initials: C
authors.lastname: Lee
authors.forename: Dongwan
authors.initials: D
authors.lastname: Yoo
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Brooks
authors.forename: M Anthony
authors.initials: MA
authors.lastname: Hayes
mesh_terms: D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D007526:Isoelectric Point; D037102:Lectins / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000187:drug effects* / Q000502:physiology; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D013552:Swine; D010948:Viral Plaque Assay; D000097604:Ficolins
chemical_list: D037102:Lectins; D011994:Recombinant Proteins; D000097604:Ficolins