title: Isolation of avian paramyxovirus 1 from a patient with a lethal case of pneumonia.
abstract: An unknown virus was isolated from a lung biopsy sample and multiple other samples from a patient who developed a lethal case of pneumonia following a peripheral blood stem cell transplant. A random PCR-based molecular screening method was used to identify the infectious agent as avian paramyxovirus 1 (APMV-1; a group encompassing Newcastle disease virus), which is a highly contagious poultry pathogen that has only rarely been found in human infections. Immunohistochemical analysis confirmed the presence of APMV-1 antigen in sloughed alveolar cells in lung tissue from autopsy. Sequence from the human isolate showed that it was most closely related to virulent pigeon strains of APMV-1. This is the most completely documented case of a systemic human infection caused by APMV-1 and is the first report of an association between this virus and a fatal disease in a human.
authors.affiliation: Divisions of Infectious Disease and Molecular Medicine, David Axelrod Institute, Wadsworth Center, NYSDOH, Albany, NY 12201-2002, USA.
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Goebel
authors.forename: Jill
authors.initials: J
authors.lastname: Taylor
authors.forename: Bradd C
authors.initials: BC
authors.lastname: Barr
authors.forename: Timothy E
authors.initials: TE
authors.lastname: Kiehn
authors.forename: Hugo R
authors.initials: HR
authors.lastname: Castro-Malaspina
authors.forename: Cyrus V
authors.initials: CV
authors.lastname: Hedvat
authors.forename: Kim A
authors.initials: KA
authors.lastname: Rush-Wilson
authors.forename: Cassandra D
authors.initials: CD
authors.lastname: Kelly
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Davis
authors.forename: William A
authors.initials: WA
authors.lastname: Samsonoff
authors.forename: Kelley R
authors.initials: KR
authors.lastname: Hurst
authors.forename: Melissa J
authors.initials: MJ
authors.lastname: Behr
authors.forename: Paul S
authors.initials: PS
authors.lastname: Masters
mesh_terms: D000328:Adult; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001717:Birds; D017809:Fatal Outcome; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000175:diagnosis* / Q000473:pathology / Q000821:virology*; D009522:Newcastle disease virus / Q000235:genetics / Q000302:isolation & purification*; D011024:Pneumonia, Viral / Q000175:diagnosis* / Q000473:pathology / Q000821:virology*; D033581:Stem Cell Transplantation / Q000009:adverse effects
chemical_list: D000956:Antigens, Viral