title: Functional connections and pathways of coenzyme Q10-inducible genes: an in-silico study.
abstract: Coenzyme Q10 (CoQ10, ubiquinone) is an essential cofactor in the electron transport chain, serves as a potent antioxidant in mitochondria and lipid membranes, and is often used as a dietary supplement for a number of diseases including cardiovascular diseases. Recently, we obtained evidence that CoQ10 (Kaneka Q10) affects the expression of hundreds of human genes. To decipher the functional and regulatory connections of these genes, a literature search combined with transcription factor binding site analysis was performed using Genomatix BiblioSphere and MatInspector. This in-silico analysis revealed 17 CoQ10-inducible genes which are functionally connected by signalling pathways of G-protein coupled receptors, JAK/STAT, integrin, and beta-arrestin. Promoter analysis of these CoQ10-inducible genes showed one group of NF B-regulated genes, namely IL5, thrombin, vitronectin receptor and C-reactive protein (CRP). Furthermore, a common promoter framework containing binding sites of the transcription factor families EVI1, HOXF, HOXC, and CLOX was identified in the promoters of IL5, CRP, and vitronectin receptor. The identified CoQ10-inducible genes and pathways play an important role in inflammatory response. Since these effects are based on an in-vitro study, the effect of CoQ10 on vascular health in vivo needs to be addressed in further animal and/or human intervention studies.
authors.affiliation: Institute of Human Nutrition and Food Science, Molecular Nutrition, Christian-Albrechts-University of Kiel, Germany. doering@molnut.uni-kiel.de
authors.forename: Constance
authors.initials: C
authors.lastname: Schmelzer
authors.forename: Inka
authors.initials: I
authors.lastname: Lindner
authors.forename: Christina
authors.initials: C
authors.lastname: Vock
authors.forename: Kenji
authors.initials: K
authors.lastname: Fujii
authors.forename: Frank
authors.initials: F
authors.lastname: Döring
mesh_terms: D018938:Caco-2 Cells; D003067:Coenzymes / Q000502:physiology; D019295:Computational Biology / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*; D012984:Software; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000502:physiology
chemical_list: D003067:Coenzymes; D014451:Ubiquinone; C024989:coenzyme Q10