title: Suppression of reactive oxygen species by glyceraldehyde-3-phosphate dehydrogenase.
abstract: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a classical glycolytic enzyme, is involved in cellular energy production and has important housekeeping functions. In this report, we show that a GAPDH from Arabidopsis, GAPDHa, has a novel function involved in H(2)O(2)-mediated cell death in yeast and Arabidopsis protoplasts. GAPDHa was cloned along with other plant genes that suppress Bax-induced cell death in yeast. Flow cytometry analyses with dihydrorhodamine 123 indicated that H(2)O(2) production mediated by Bax expression in yeast cells was greatly reduced when Bax was coexpressed with GAPDHa. In plants, GAPDHa transcript levels were greatly increased by H(2)O(2) treatment. Furthermore, transformation of GAPDHa into Arabidopsis protoplasts strongly suppressed heat shock-induced H(2)O(2) production and cell death. Together, our results indicate that GAPDH controls generation of H(2)O(2) by Bax and heat shock, which in turn suppresses cell death in yeast and plant cells.
authors.affiliation: Division of Applied Life Science (BK21 program) and Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Dongwon
authors.initials: D
authors.lastname: Baek
authors.forename: Yinhua
authors.initials: Y
authors.lastname: Jin
authors.forename: Jae Cheol
authors.initials: JC
authors.lastname: Jeong
authors.forename: Hyo-Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Haejeong
authors.initials: H
authors.lastname: Moon
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Lee
authors.forename: Dongjin
authors.initials: D
authors.lastname: Shin
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Kang
authors.forename: Doh Hoon
authors.initials: DH
authors.lastname: Kim
authors.forename: Jaesung
authors.initials: J
authors.lastname: Nam
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.forename: Dae-Jin
authors.initials: DJ
authors.lastname: Yun
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology / Q000235:genetics; D016923:Cell Death; D015971:Gene Expression Regulation, Enzymologic; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress; D011523:Protoplasts / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases