A high-sensitive amperometric hydrogen peroxide biosensor based on the immobilization of hemoglobin on gold colloid/L-cysteine/gold colloid/nanoparticles Pt-chitosan composite film-modified platinum disk electrode.
A hemoglobin (Hb)/gold colloid (nano-Au)/L-cysteine (L-cys)/nano-Au/nanoparticles Pt (nano-Pt)-chitosan (CHIT) composite film-modified platinum disk electrode (abbreviated to modified electrode) has been prepared to construct a biosensor for determination of H(2)O(2). The electrochemical characteristics of the biosensor were studied by cyclic voltammetry and chronoamperometry. The modified process was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The morphologies of different composite film were investigated with scanning electron microscopy (SEM) and the element of composite film was investigated with X-ray photoelectron spectroscopy (XPS). Analytical parameters such as pH and temperature were also studied. The linear range for the determination of H(2)O(2) is 1.4 x 10(-7) to 6.6 x 10(-3)mol/L with a detection limit of 4.5 x 10(-8)mol/L (S/N=3). The sensor achieved 95% of the steady-state current within 10s. The sensor exhibited high sensitivity (17.62 microA/(mmol L)), selectivity and stability. The method is applied to the determination of H(2)O(2) with satisfactory results.
Yang
Gan
G
Chongqing Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
Yuan
Ruo
R
Chai
Ya-Qin
YQ
D015374:Biosensing Techniques / Q000295:instrumentation*; D048271:Chitosan / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D017979:Gold Colloid; D006454:Hemoglobins / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning; D010984:Platinum / Q000737:chemistry*; D015203:Reproducibility of Results; D013696:Temperature
D017979:Gold Colloid; D006454:Hemoglobins; D010984:Platinum; D048271:Chitosan; D006861:Hydrogen Peroxide; D003545:Cysteine