Production of trans-2-methyl-5-isopropylhexa-2,5-dienoic acid by Pseudomonas rhodesiae CIP 107491.
The feasibility of trans-2-methyl-5-isopropylhexa-2,5-dienoic acid (novalic acid) accumulation using the alpha-pinene degradation pathway of Pseudomonas rhodesiae CIP 107491 was studied. This appeared possible by using concentrated living bacterial cells produced under oxygen limitation with alpha-pinene as sole carbon source. The second step of the process, the bioconversion itself, had to be performed without oxygen limitation due to the need for cofactor regeneration. Results showed that a not yet reported cofactor-dependent enzymatic isomerization of isonovalal into novalal was likely to occur and that both aldehyde isomers could be oxidized to the corresponding acid. Precursors tested, alpha-pinene oxide and isonovalal had a strong permeabilization effect on bacterial cells. This effect, which increased from the oxide to the aldehyde, led to an inactivation of the respiratory chain and to acids synthesis stop. Present results allowed to obtain about 12 g/L acids (80% novalic acid) with an average yield close to 50% after 12h reaction in a biphasic system using alpha-pinene oxide as precursor .
Linares
Denis
D
Laboratoire de Génie Chimique et Biochimique, Polytech'Clermont-Ferrand, Université Blaise Pascal, BP 206, F-63174 Aubière Cedex, France.
Martinez
Daniela
D
Fontanille
Pierre
P
Larroche
Christian
C
D000447:Aldehydes / Q000633:toxicity; D000081008:Bicyclic Monoterpenes; D018533:Biomass; D019149:Bioreactors; D002244:Carbon / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D050296:Microbial Viability / Q000187:drug effects; D039821:Monoterpenes; D010100:Oxygen / Q000378:metabolism; D011549:Pseudomonas / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013011:Sorbic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D013729:Terpenes / Q000633:toxicity; D013997:Time Factors
D000447:Aldehydes; D000081008:Bicyclic Monoterpenes; D005231:Fatty Acids, Unsaturated; D039821:Monoterpenes; D013729:Terpenes; C473705:isonovalal; C530422:trans-2-methyl-5-isopropylhexa-2,5-dienoic acid; C053997:2-pinene oxide; D002244:Carbon; D010100:Oxygen; D013011:Sorbic Acid