Mutational analysis of ErbB2 intracellular localization.
In the present study, the sub-cellular localization of ErbB2 and its mutants expressed as GFP-tagged proteins in MCF-7 cells or endogenous ErbB2 in SKBR3 cells was examined. The data presented here demonstrate that the full-length ErbB2 was localized at the cytoplasmic membrane and ErbB2 ICD localized in the nucleus predominantly. The sequence of ErbB2 ICD contains the information supporting its nuclear translocation and cytoplasmic retention. A region (residues 721-970) harboring an arginine triplet is essential for the cytoplasmic trafficking of ErbB2. The results indicate that differential sub-cellular localization of ErbB2 ICD and the full-length ErbB2 is dependent on their structural determinants. The present results give initial clues for further analysis of the mechanism of ErbB2 intracellular localization.
Chen
Liyong
L
Institute of Basic Medical Sciences, Taiping Road 27, Beijing, 100850, People's Republic of China.
Qian
Lu
L
Zhang
Zhiyi
Z
Shi
Ming
M
Song
Yuhua
Y
Yuan
Guogang
G
Zhang
Hao
H
Hu
Meiru
M
Yu
Ming
M
Zhang
Xuemin
X
Shen
Beifen
B
Guo
Ning
N
D000818:Animals; D016466:CHO Cells; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D004252:DNA Mutational Analysis; D006801:Humans; D019913:Nuclear Localization Signals / Q000235:genetics; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D014407:Tumor Cells, Cultured
D019913:Nuclear Localization Signals; D018719:Erb-b2 Receptor Tyrosine Kinases