Toxicity, binding and internalization of the pea-A1b entomotoxin in Sf9 cells.
PA1b (Pea Albumin 1b) is a peptide toxin lethal for certain insects. This paper shows that the cultured insect cells Sf9 are sensitive to the toxin and display a high-affinity binding site for PA1b. Mammalian cells are not sensitive and no binding activity was detected. Signs of apoptosis of the Sf9 cells were observed in response to the toxin. The use of this cellular model also demonstrated that PA1b was internalized in the cells, via the binding site, raising the new question of the role of this toxin within the cell, and of the mechanisms leading to cell death.
Rahioui
Isabelle
I
UMR203 Biologie Fonctionnelle Insectes et Interactions, IFR41, INRA, INSA de Lyon, INSA Bâtiment Louis Pasteur, F-69100 Villeurbanne Cedex, France.
Laugier
Christian
C
Balmand
Séverine
S
Da Silva
Pedro
P
Rahbe
Yvan
Y
Gressent
Frédéric
F
D000418:Albumins / Q000737:chemistry*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001665:Binding Sites; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004731:Endotoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D007457:Iodine Radioisotopes / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D018532:Pisum sativum / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry; D011485:Protein Binding; D020033:Protein Isoforms / Q000737:chemistry; D018411:Spodoptera / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D013696:Temperature; D013997:Time Factors
D000418:Albumins; D004731:Endotoxins; D007457:Iodine Radioisotopes; D010940:Plant Proteins; D020033:Protein Isoforms