[Study on histogenesis of enterochromaffin-like carcinoid in autoimmune atrophic gastritis associated with pernicious anemia].
OBJECTIVE
Autoimmune atrophic fundic gastritis induces the pernicious anemia (PA), as well as the changes in both epithelium and endocrine cells of gastric mucosa. The most important complications are: achlorhydria, hypergastrinemia, gastric cancer and enterochromaffin-like (ECL) carcinoid. The aim of this study was to examine ECL carcinoid histogenesis in A-gastritis associated with PA.


METHODS
During the period from 2000-2006, 65 patients with PA and 30 patients of the control group were examined. Histopathological examination was done in endoscopical biopsies of gastric mucosa fixed in 10% formaldehyde. Paraffin sections were stained with classic hematoxylin-eosin (HE); histochemical AB-PAS (pH 2.5), cytochemical argyrophilic Servier-Munger's and immunocytochemical PAP methods for G cell identification and chromogranin A antibodies - specific marker for neuroendocrine ECL cells. Both G and ECL cells were counted per 20 fields, of surface 0.0245312 mm2 by a field. Basal gastrin serum levels were also examined by using radioimmunoassay (RIA) method. The obtained results were statisticaly calculated by using Student's t test.


RESULTS
Marked antral G cell hyperplasia associated with corporal ECL hyperplasia was found. ECL cell hyperplasia was of simplex, linear, adenomatoid type to the pattern of intramucous ECL cell carcinoid. An average number of G cells was statistically significant in the patients with PA as compared to the control group (p < 0.05) as well as an average number of ECL cells.


CONCLUSIONS
We concluded that antral G cell hyperplasia accompanied by gastrinemia induces ECL hyperplasia and ECL corporal carcinoid in A-gastritis and that their histogenesis develops trough simple, linear and adenomatoide hyperplasia.
Macukanović-Golubović
Lana
L
Medicinski fakultet, Klinika za hematologiju, Bulevar Zorana Dindića 48, 18 000 Nis, Srbija. golubovicz@bankerinter.net
Katić
Vuka
V
Rancić
Gorana
G
Milenović
Mladen
M
Marjanović
Goran
G
Golubović
Zoran
Z
D000752:Anemia, Pernicious / Q000209:etiology*; D001327:Autoimmune Diseases / Q000473:pathology*; D002276:Carcinoid Tumor / Q000209:etiology / Q000473:pathology*; D019861:Enterochromaffin-like Cells / Q000473:pathology*; D005753:Gastric Mucosa / Q000473:pathology*; D019863:Gastrin-Secreting Cells / Q000473:pathology; D005757:Gastritis, Atrophic / Q000150:complications*; D006801:Humans; D006965:Hyperplasia; D013274:Stomach Neoplasms / Q000209:etiology / Q000473:pathology*