Characterization of cellular chemical dynamics using combined microfluidic and Raman techniques.
The integration of a range of technologies including microfluidics, surface-enhanced Raman scattering and confocal microspectroscopy has been successfully used to characterize in situ single living CHO (Chinese hamster ovary) cells with a high degree of spatial (in three dimensions) and temporal (1 s per spectrum) resolution. Following the introduction of a continuous flow of ionomycin, the real time spectral response from the cell was monitored during the agonist-evoked Ca(2+) flux process. The methodology described has the potential to be used for the study of the cellular dynamics of a range of signalling processes.
Zhang
Xunli
X
Department of Chemistry, The University of Hull, Hull, HU6 7RX, UK.
Yin
Huabing
H
Cooper
Jon M
JM
Haswell
Stephen J
SJ
D000818:Animals; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D002623:Chemistry Techniques, Analytical / Q000379:methods; D006224:Cricetinae; D003412:Cricetulus; D004867:Equipment Design; D017979:Gold Colloid / Q000737:chemistry; D021621:Imaging, Three-Dimensional; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics; D013059:Spectrum Analysis, Raman / Q000379:methods*
D017979:Gold Colloid; D002118:Calcium