title: Evaluation of MChip with historic subtype H1N1 influenza A viruses, including the 1918 "Spanish Flu" strain.
abstract: The robustness of a recently developed diagnostic microarray for influenza, the MChip, was evaluated with 16 historic subtype H1N1 influenza A viruses (A/H1N1), including A/Brevig Mission/1/1918. The matrix gene segments from all 16 viruses were successfully detected on the array. An artificial neural network trained with temporally related A/H1N1 viruses identified A/Brevig Mission/1/1918 as influenza virus A/H1N1 with 94% probability.
authors.affiliation: Department of Chemistry and Biochemistry, UCB 215, University of Colorado at Boulder, Boulder, CO 80309, USA.
authors.forename: Chad L
authors.initials: CL
authors.lastname: Moore
authors.forename: James A
authors.initials: JA
authors.lastname: Smagala
authors.forename: Catherine B
authors.initials: CB
authors.lastname: Smith
authors.forename: Erica D
authors.initials: ED
authors.lastname: Dawson
authors.forename: Nancy J
authors.initials: NJ
authors.lastname: Cox
authors.forename: Robert D
authors.initials: RD
authors.lastname: Kuchta
authors.forename: Kathy L
authors.initials: KL
authors.lastname: Rowlen
mesh_terms: D053118:Influenza A Virus, H1N1 Subtype / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016571:Neural Networks, Computer; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*