Light scattering sensing detection of pathogens based on the molecular recognition of immunoglobulin with cell wall-associated protein A.
In this contribution, we report a rapid optical detection method of pathogens using Staphylococcus aureus (S. aureus) as the model analyte based on the molecular recognition of immunoglobulin with cell wall-associated Protein A (SpA). It was found that the molecular recognition of human immunoglobulin (IgG) with protein A on the cell wall of S. aureus on glass slide sensing area could result in strong surface enhanced light scattering (SELS) signals, and the SELS intensity (deltaI) increases proportionally with the concentration of S. aureus over the range of 2.5x10(5)-1.0x10(8) CFU mL(-1) with right angle light scattering (RALS) signals detection mode. In order to identify the solid support based molecular recognition between IgG with SpA, we also employed water-soluble CdS quantum dots (CdS-QDs) as a fluorescent marker for IgG by immobilizing the IgG onto the surfaces of CdS-QDs through covalent binding in order to generate recognition probes for SpA on the cell wall of S. aureus. Consequently, the fluorescent method also showed that the detection for pathogens with solid supports is reliable based on the molecular recognition of IgG with SpA.
Liu
Zhong De
ZD
College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
Chen
Shao Fen
SF
Huang
Cheng Zhi
CZ
Zhen
Shu Jun
SJ
Liao
Qie Gen
QG
D001413:Bacillus thuringiensis / Q000302:isolation & purification; D015374:Biosensing Techniques / Q000379:methods*; D004926:Escherichia coli / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000032:analysis*; D045663:Quantum Dots; D012542:Scattering, Radiation; D013205:Staphylococcal Protein A / Q000032:analysis*; D013211:Staphylococcus aureus / Q000302:isolation & purification*
D007074:Immunoglobulin G; D013205:Staphylococcal Protein A