Comparison of rapid DNA extraction methods applied to PCR identification of medicinal mushroom Ganoderma spp.
Four different DNA extraction methods were used to extract genomic DNA of the medicinal mushroom Lingzhi from its developing stage materials, such as mycelium, dry fruiting body, or sliced and spore powder or sporoderm-broken spore powder. The DNA samples were analyzed using agarose gel electrophoresis, UV spectrophotometer, and PCR amplification. According to the average yields and purity of DNA, high salt concentrations and low pH methods were the best for DNA extraction. The mycelia and sporoderm-broken spore powder yielded higher and purer DNA. The method developed could effectively eliminate the influence of the secondary metabolites to DNA extraction. The DNA samples extracted from the developed method could be successfully used for PCR applications.
Zhou
Xuanwei
X
Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center Shanghai Jiao Tong University, Shanghai, PR China.
Li
Qizhang
Q
Zhao
Jingya
J
Tang
Kexuan
K
Lin
Juan
J
Yin
Yizhou
Y
D001709:Biotechnology / Q000379:methods*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D032902:Ganoderma / Q000737:chemistry* / Q000235:genetics; D016681:Genome, Fungal; D008518:Medicine, East Asian Traditional; D025282:Mycelium / Q000737:chemistry; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D013172:Spores, Fungal / Q000737:chemistry; D013997:Time Factors
D004247:DNA