[Mechanism of salvianolate in preventing postoperative intestinal adhesion in rats].
OBJECTIVE
To observe the effects of salvianolate on rats with postoperative intestinal adhesion and to explore the prevention mechanism.


METHODS
Forty SD male rats with intestinal adhesion were randomly divided into four groups: untreated group, low-dose salvianolate-treated group (12 mg/kg), medium-dose salvianolate-treated group (24 mg/kg) and high-dose salvianolate-treated group (48 mg/kg), with another ten SD male rats as normal control. Intraperitoneal injection of glucose was administered to the rats in the normal control group and the untreated group, and intraperitoneal injection of salvianolate was administered to the rats in the low-, medium- and high-dose salvianolate-treated groups. They were all treated for 8 days and once a day. On the eighth day after surgery the blood samples of each group were collected. Grades of intestinal adhesion were ranked by macroscopic observation. The adhesive tissues between viscera and belly wall were taken for pathological observation. The levels of interleukin-1beta (IL-1beta), interleukin-4 (IL-4) and tumor necrosis factor-alpha (TNF-alpha) were determined by enzyme linked immunosorbent assay.


RESULTS
Salvianolate can significantly reduce the extent of postoperative intestinal adhesion, obviously decrease the levels of IL-1beta, TNF-alpha and inhibit the hyperplasy of fibrous connective tissue. However, there was no significant impact on the level of IL-4.


CONCLUSIONS
Salvianolate can reduce the extent of postoperative intestinal adhesion, decrease the expression of IL-1beta and TNF-alpha and inhibit the hyperplasy of fibrous connective tissue. This may be the mechanism of salvianolate in preventing intestinal adhesion.
Sui
Xin-bing
XB
First Clinical Medical College, Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang Province 310053, China.
Zhang
Qin
Q
Qiu
Hua-sen
HS
Zhou
Ji-chun
JC
Gu
Xi-dong
XD
Lu
Zhou-xiang
ZX
Li
Chao
C
Jin
Li-jun
LJ
Wang
Gang
G
D000005:Abdomen / Q000601:surgery*; D000818:Animals; D002109:Caffeic Acids / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000378:metabolism; D007410:Intestinal Diseases / Q000378:metabolism / Q000517:prevention & control*; D007773:Lactates / Q000494:pharmacology*; D008297:Male; D011183:Postoperative Complications / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry; D000267:Tissue Adhesions / Q000378:metabolism / Q000517:prevention & control; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D002109:Caffeic Acids; D053583:Interleukin-1beta; D007773:Lactates; D014409:Tumor Necrosis Factor-alpha; C066201:salvianolic acid A