title: The CYP3A4 *1B polymorphism and prostate cancer susceptibility in a Portuguese population.
abstract: Testosterone exposure has been implicated in prostate carcinogenesis, and genes that alter its metabolism, such as CYP3A4, have been associated with prostate cancer susceptibility. The aim of our study was to assess the relationship between the CYP3A4 *1B polymorphism and its possible role in the development of prostate cancer. DNA samples obtained from the peripheral blood cells of 414 individuals diagnosed with prostate cancer and 337 healthy male donors were used in this case-control study. The CYP3A4*1B polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism methodology. We found no statistically significant differences in the distribution of the CYP3A4*1B genotypes between cases and controls (P = 0.470; odds ratio = 1.191; 95% confidence interval=0.740-1.918), as well as after the stratification of our analysis, according to important clinicopathologic parameters of prostate cancer. Our results suggest that the CYP3A4*1B polymorphism is not associated with prostate cancer risk within the Portuguese population.
authors.affiliation: Molecular Oncology Department, Portuguese Institute of Oncology, Rua Dr. António Bernardino Almeida, 4200-072 Porto, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Nogal
authors.forename: Ana
authors.initials: A
authors.lastname: Coelho
authors.forename: Raquel
authors.initials: R
authors.lastname: Catarino
authors.forename: António
authors.initials: A
authors.lastname: Morais
authors.forename: Francisco
authors.initials: F
authors.lastname: Lobo
authors.forename: Rui
authors.initials: R
authors.lastname: Medeiros
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D051544:Cytochrome P-450 CYP3A; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004247:DNA / Q000097:blood / Q000235:genetics; D004198:Disease Susceptibility; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011174:Portugal / Q000453:epidemiology; D011379:Prognosis; D011467:Prostate / Q000201:enzymology; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000453:epidemiology / Q000235:genetics*; D044465:White People
chemical_list: D004247:DNA; D003577:Cytochrome P-450 Enzyme System; D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human