title: A high-resolution atlas of nucleosome occupancy in yeast.
abstract: We present the first complete high-resolution map of nucleosome occupancy across the whole Saccharomyces cerevisiae genome, identifying over 70,000 positioned nucleosomes occupying 81% of the genome. On a genome-wide scale, the persistent nucleosome-depleted region identified previously in a subset of genes demarcates the transcription start site. Both nucleosome occupancy signatures and overall occupancy correlate with transcript abundance and transcription rate. In addition, functionally related genes can be clustered on the basis of the nucleosome occupancy patterns observed at their promoters. A quantitative model of nucleosome occupancy indicates that DNA structural features may account for much of the global nucleosome occupancy.
authors.affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, California 94305-5120, USA.
authors.forename: William
authors.initials: W
authors.lastname: Lee
authors.forename: Desiree
authors.initials: D
authors.lastname: Tillo
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bray
authors.forename: Randall H
authors.initials: RH
authors.lastname: Morse
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Davis
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Hughes
authors.forename: Corey
authors.initials: C
authors.lastname: Nislow
mesh_terms: D016681:Genome, Fungal; D009707:Nucleosomes / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins