[Correlation analysis of relationships between polymorphisms of high quality chicken myogenin gene and slaughter and meat quality traits].
PCR-SSCP technique was designed in this study to investigate the effect of MyoG on quality meat chicken (developed by Sichuan Dahen Poultry Breeding Company using local breeds). Four mutations at base position in promoter were detected among individuals in each line, i.e. T/C in locusA and T/A, T/C and A/G in locus B . The least square analysis showed that there were a significant difference between genotypes and breast muscle percentage and some carcass traits (P<0.05for locus A. There were a significant difference (P <0.05) in breast muscle weight between ACAA and AB geno- types; a significant difference (P <0.05) in leg muscle percentage between CC and AC for locus B, and a extremity signifi-cant difference (P <0.01) in the frequency of genotype muscle Fibre Density for Bothlocus A to locus B. There was no sig-nificant difference (P >0.05) in the other triats. It was concluded from the results that MyoG gene is the major gene affect-ing the muscle fiber traits of chicken or it links with the candidate gene, and the mutation could be used as the molecular genetic marker to select the chickens for Meat Quality traits.
Wang
Qiong
Q
Animal Science and Technology College of Sichuan Agriculture University, Ya'an 625014, China. goldensky365@yahoo.com.cn
Liu
Yi-Ping
YP
Jiang
Xiao-Song
XS
Yang
Chao-Wu
CW
DU
Hua-Rui
HR
Qiu
Mo-Han
MH
Zhu
Qing
Q
D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D008460:Meat / Q000592:standards*; D018008:Myogenin / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011786:Quality Control; D017422:Sequence Analysis, DNA
D018008:Myogenin