TRRAP and GCN5 are used by c-Myc to activate RNA polymerase III transcription.
Activation of RNA polymerase (pol) II transcription by c-Myc generally involves recruitment of histone acetyltransferases and acetylation of histones H3 and H4. Here, we describe the mechanism used by c-Myc to activate pol III transcription of tRNA and 5S rRNA genes. Within 2 h of its induction, c-Myc appears at these genes along with the histone acetyltransferase GCN5 and the cofactor TRRAP. At the same time, occupancy of the pol III-specific factor TFIIIB increases and histone H3 becomes hyperacetylated, but increased histone H4 acetylation is not detected at these genes. The rapid acetylation of histone H3 and promoter assembly of TFIIIB, c-Myc, GCN5, and TRRAP are followed by recruitment of pol III and transcriptional induction. The selective acetylation of histone H3 distinguishes pol III activation by c-Myc from mechanisms observed in other systems.
Kenneth
Niall S
NS
Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
Ramsbottom
Ben A
BA
Gomez-Roman
Natividad
N
Marshall
Lynne
L
Cole
Philip A
PA
White
Robert J
RJ
D016475:3T3 Cells; D000107:Acetylation; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D004789:Enzyme Activation; D006367:HeLa Cells; D051548:Histone Acetyltransferases / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D006877:Hydroxamic Acids / Q000378:metabolism; D000074443:Lysine Acetyltransferase 5; D051379:Mice; D008807:Mice, Inbred BALB C; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D012320:RNA Polymerase III / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism; D035361:Transcription Factor TFIIIB / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D014162:Transfection; D050880:p300-CBP Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000099224:p300-CBP-Associated Factor
D048868:Adaptor Proteins, Signal Transducing; D051548:Histone Acetyltransferases; D006657:Histones; D006877:Hydroxamic Acids; D000074443:Lysine Acetyltransferase 5; D009687:Nuclear Proteins; D016271:Proto-Oncogene Proteins c-myc; D012320:RNA Polymerase III; D012343:RNA, Transfer; D035361:Transcription Factor TFIIIB; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C489427:MYC protein, human; C113829:transformation-transcription domain-associated protein; C012589:trichostatin A; C098489:KAT5 protein, human