Development of a bacterial surface display of hexahistidine peptide using CS3 pili for bioaccumulation of heavy metals.
A novel cell surface display system for metal uptake was developed using CS3 pili of enterotoxigenic Escherichia coli, which is a suitable system for display of heterologous peptides. The recombinant bacteria producing the hybrid pili containing the hexahistidine peptide accumulated high concentrations of Cd(2+) and Ni(2+) at 656.2 and 276.5 nmol per mg dry weight of bacterial cell, respectively. The recombinant bacteria may be useful in water and waste water treatment.
Saffar
Behnaz
B
National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Yakhchali
Bagher
B
Arbabi
Mehdi
M
D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D002462:Cell Membrane / Q000378:metabolism; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics* / Q000378:metabolism; D004785:Environmental Pollutants / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D036781:Fimbriae Proteins / Q000235:genetics* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006639:Histidine / Q000235:genetics* / Q000378:metabolism; D019216:Metals, Heavy / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D004785:Environmental Pollutants; D029968:Escherichia coli Proteins; C471213:His-His-His-His-His-His; D019216:Metals, Heavy; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins; C081391:nfaE protein, E coli; D036781:Fimbriae Proteins; D006639:Histidine