title: [Effects of total saponins of Panax japonicus on human leukemic HL-60 cells].
abstract: OBJECTIVE
  To study the effects of total saponins of Panax japonicus (TSPJ) on human leukemic HL-60 cells.
  
  
  METHODS
  Human leukemic HL-60 cells were cultured in vitro. The cancer cell vigor was detected by using cell counting kit-8. Nitroblue tetrazolium (NBT) was used for measuring cell reduction. The cell cycle and the expression of differentiation antigen CD11b were detected by flow cytometry.
  
  
  RESULTS
  Compared with the negative control group, TSPJ in different concentrations could decrease the vigor of HL-60 cells and the number of cells in S phase and up-regulate the CD11b expression, while the numbers of NBT positive cells and cells in G(0)/G(1) phase in the different concentrations of TSPJ-treated groups were increased.
  
  
  CONCLUSIONS
  TSPJ can inhibit the HL-60 cell growth in vitro. Its mechanism may be related to inhibiting proliferation and inducing cell differentiation and cycle arrest.
authors.affiliation: Medical College, Three Gorges University, Yichang, Hubei Province 443002, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Yuan
authors.forename: Rui
authors.initials: R
authors.lastname: Zuo
authors.forename: Chang-cheng
authors.initials: CC
authors.lastname: Zhang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D039481:CD11b Antigen / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D009580:Nitroblue Tetrazolium / Q000032:analysis; D005894:Panax / Q000737:chemistry*; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D039481:CD11b Antigen; D012503:Saponins; D009580:Nitroblue Tetrazolium