title: Two-photon microscopy and spectroscopy of lanthanide bioprobes.
abstract: The linear and non-linear photophysical properties of tris-dipicolinate europium and terbium complexes (absorption, emission, lifetime, luminescence induced by two-photon absorption) are studied in the crystalline state as well as in protein derivative crystals and compared to those in solution. Upon laser irradiation at 532 nm, luminescence of terbium is induced by a two-photon antenna effect, whereas luminescence of europium results from one-photon absorption in forbidden f-f transitions. Finally, linear and two-photon microscopy imaging experiments on biological and bio-inspired crystals are performed. These first proof-of-concept experiments open the way for the development of time-resolved non-linear microscopy that should combine the advantages of lanthanide luminescence (long lifetime, sharp emission bands, insensitivity to oxygen) with those of confocal biphotonic excitation (near-IR excitation, 3D resolution and reduced photodamage).
authors.affiliation: Laboratoire de Chimie, ENS-Lyon, CNRS, 46 Allée d'Italie, 69364 Lyon, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: D'Aléo
authors.forename: Guillaume
authors.initials: G
authors.lastname: Pompidor
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Elena
authors.forename: Jean
authors.initials: J
authors.lastname: Vicat
authors.forename: Patrice L
authors.initials: PL
authors.lastname: Baldeck
authors.forename: Loïc
authors.initials: L
authors.lastname: Toupet
authors.forename: Richard
authors.initials: R
authors.lastname: Kahn
authors.forename: Chantal
authors.initials: C
authors.lastname: Andraud
authors.forename: Olivier
authors.initials: O
authors.lastname: Maury
mesh_terms: D001703:Biophysics / Q000379:methods; D015374:Biosensing Techniques; D002627:Chemistry, Physical / Q000379:methods; D003460:Crystallization; D005063:Europium / Q000737:chemistry; D028581:Lanthanoid Series Elements / Q000494:pharmacology*; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D018613:Microscopy, Confocal; D010777:Photochemistry; D017785:Photons; D013055:Spectrophotometry, Infrared / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013725:Terbium / Q000737:chemistry; D013997:Time Factors
chemical_list: D028581:Lanthanoid Series Elements; D013725:Terbium; D005063:Europium