Transfer printing of DNA by "click" chemistry.
This paper describes a straightforward procedure to immobilize oligonucleotides on glass substrates in well-defined micropatterns by microcontact printing with a dendrimer-modified stamp. The oligonucleotides are efficiently immobilized by "click" chemistry induced by microcontact printing. Acetylene-modified oligonucleotides were treated with an azide-terminated glass slide under the confinement of the dendrimer-modified stamp, without the use of a Cu(I) catalyst. The immobilization is an irreversible, covalent, and one-step reaction that results in stable attachment of the oligonucleotides. Oligonucleotides with the acetylene-modification at the 5' terminus hybridize selectively with full-length, complementary targets. Strands with more than one acetylene linker do not hybridize with complementary strands.
Rozkiewicz
Dorota I
DI
Laboratory of Supramolecular Chemistry and Technology, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
Gierlich
Johannes
J
Burley
Glenn A
GA
Gutsmiedl
Katrin
K
Carell
Thomas
T
Ravoo
Bart Jan
BJ
Reinhoudt
David N
DN
D000114:Acetylene / Q000737:chemistry; D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D008968:Molecular Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D010316:Particle Size; D013329:Structure-Activity Relationship; D013499:Surface Properties; D013844:Thiazoles / Q000737:chemistry
D050091:Dendrimers; D008567:Membranes, Artificial; D009841:Oligonucleotides; C086329:TOTO-1 thiazole orange dye; D013844:Thiazoles; D004247:DNA; D000114:Acetylene