Antifibrotic effect via the regulation of transcription factor Sp1 in lung fibrosis.
The aim of this study is to evaluate the antifibrotic effect of ring-type Sp1 decoy oligonucleotides (ODNs) through blocking the transcription of transforming growth factor (TGF)-beta1 and its downstream target genes. In this experiment, the expression of TGF-beta1, metalloproteinase (MMP)-13, and fibronectin was decreased in the group with the treatment of the ring-type Sp1 decoy ODNs. Also, alpha-smooth muscle actin positive bronchial lining cells and alveolar epithelial cells were observed, especially around the lesions of extracellular matrix (ECM) deposition. These findings provide evidences for the finding of pulmonary epithelial-mesenchymal transition (EMT) and the effectiveness of Sp1 transcription factor as a target for the gene therapy on lung fibrosis.
Kum
Yoon-Seup
YS
Department of Pathology, Catholic University of Daegu, College of Medicine, 3056-6, Daemyung 4-Dong, Nam-Gu, Daegu 705-718, Republic of Korea.
Kim
Kyung-Hyun
KH
Park
Tae-In
TI
Suh
In-Soo
IS
Oh
Hoon-Kyu
HK
Cho
Chang-Ho
CH
Park
Jae-Bok
JB
Chang
Young-Chae
YC
Park
Ji-Hyun
JH
Lee
Kwang-Gil
KG
Park
Kwan-Kyu
KK
D000818:Animals; D018390:Gene Targeting / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009841:Oligonucleotides / Q000008:administration & dosage* / Q000235:genetics*; D011658:Pulmonary Fibrosis / Q000235:genetics / Q000378:metabolism* / Q000628:therapy*; D016329:Sp1 Transcription Factor / Q000235:genetics*; D016896:Treatment Outcome
D009841:Oligonucleotides; D016329:Sp1 Transcription Factor