Application of the particle gel agglutination system as a new check gel assay for PCR products.
In this study, we describe a simple and rapid agglutination test for the detection of PCR products prior to the application of specific hybridization by sequence-specific oligonucleotide typing. This test is based on the particle gel agglutination immunoassay, incorporating biotinylated primers and streptavidin particles. Visually detectable agglutination was only observed in samples which contained the specific amplification products. The results obtained by the new test were in accordance with those obtained by standard gel electrophoresis in all cases that have been tested to date.
Schönemann
C
C
Institute of Transfusion Medicine, Charité University Medicine Berlin, Berlin, Germany. contanze.schoenemann@charite.de
Abou-Chaker
K
K
Bombard
S
S
Meyer
O
O
Salama
A
A
D000372:Agglutination Tests; D004247:DNA / Q000032:analysis*; D005782:Gels; D006680:HLA Antigens / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction
D005782:Gels; D006680:HLA Antigens; D004247:DNA