Physical exercise-associated gene expression signatures in peripheral blood.
OBJECTIVE
To assess response to physical stress, gene expression profiles in peripheral blood cells were analyzed using an original microarray carrying 1467 stress-responsive complementary DNA probes.


METHODS
Gene expression was analyzed at 4, 24, and 48 hours after exercising on a cycle ergometer at 60% VO2 max for 1 hour (aerobic exercise) or until exhausted (exhaustive exercise).


METHODS
Institute of Health Biosciences, University of Tokushima Graduate School.


METHODS
Twelve healthy male students of the postgraduate or undergraduate school.


METHODS
The volunteers performed the aerobic or exhaustive exercise on a cycle ergometer.


METHODS
Detection of aerobic exercise-responsive or exhaustive exercise-responsive genes in peripheral blood cells.


RESULTS
Aerobic and exhaustive exercise transiently changed the expression of 21 and 16 genes, respectively, with the peak at 4 hours. Only 2 genes significantly responded to both types of exercise. Exhaustive but not aerobic exercise produced a secondary response with significantly altered expression of 14 genes at 24 hours. Five of those genes encode receptors for neurotransmitters (HTR1A, CHRNB2, GABRB3, GABRG3, and LOC51289).


CONCLUSIONS
The behavior of the individual genes shown here may be informative to objectively assess acute physical stress and exhaustion-associated responses.
Kawai
Tomoko
T
Department of Stress Science, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
Morita
Kyoko
K
Masuda
Kiyoshi
K
Nishida
Kensei
K
Sekiyama
Atsuo
A
Teshima-Kondo
Shigetada
S
Nakaya
Yutaka
Y
Ohta
Masayuki
M
Saito
Toshiro
T
Rokutan
Kazuhito
K
D000222:Adaptation, Physiological / Q000235:genetics* / Q000502:physiology; D000328:Adult; D001773:Blood Cells; D016552:Ergometry; D015444:Exercise / Q000502:physiology*; D015870:Gene Expression; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011446:Prospective Studies; D012333:RNA, Messenger; D013997:Time Factors
D012333:RNA, Messenger