title: An amine: hydroxyacetone aminotransferase from Moraxella lacunata WZ34 for alaninol synthesis.
abstract: An amine:hydroxyacetone aminotransferase from an isolated soil bacterium, Moraxella lacunata WZ34, was employed to synthesize alaninol in the presence of hydroxyacetone and isopropylamine in this study. The optimal carbon and nitrogen sources were glycerol and beef extract, respectively. A wide range of amino donor specificity was detected with the aminotransferase, which exhibited a relative high activity (9.83 U mL(-1)) in the presence of isopropylamine. The enzyme was the most active at pH 8.5, and showed relatively higher activity at alkaline than acidic pH. Maximum activity was achieved at 30 degrees C, and the enzyme had good thermal stability below 60 degrees C. Metal ions such as Mg(2+) had positive effect (132.6%) on the enzyme, and (aminooxy)acetic acid, a typical aminotransferase inhibitor, significantly inhibited its activity. The enzyme activity was enhanced by the addition of 0.05 mM pyridoxal-5'-phosphate (PLP).
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Chen
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
authors.forename: Yinjun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zeyu
authors.initials: Z
authors.lastname: Sun
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D000096:Acetone / Q000031:analogs & derivatives* / Q000737:chemistry; D001679:Biogenic Amines / Q000737:chemistry*; D004789:Enzyme Activation; D009016:Moraxella / Q000201:enzymology*; D011412:Propanolamines / Q000138:chemical synthesis*; D011437:Propylamines / Q000737:chemistry*
chemical_list: D001679:Biogenic Amines; D011412:Propanolamines; D011437:Propylamines; D000096:Acetone; C034448:2-aminopropanol; C004433:acetol; C035263:2-propylamine