FGF-4 increases in vitro expansion rate of human adult bone marrow-derived mesenchymal stem cells.
Human bone marrow-derived mesenchymal stem cells (MSCs) exhibit limited in vitro growth. Fibroblast growth factors (FGFs) elicit a variety of biological responses, such as cell proliferation, differentiation and migration. FGF-4 represents one of the FGFs with the highest cell mitogenic activity. We studied the effect of FGF-4 on MSCs growth and pluripotency. MSCs duplication time (Td) was significantly reduced with FGF-4 compared to controls (2.2 +/- 0.2 vs. 4.1 +/- 0.2 days, respectively; p = 0.03) while BMP-2 and SCF-1 did not exert a significant growth effect. MSC expression of surface markers, differentiation into adipogenic and osteogenic lineages, and baseline expression of cardiomyogenic genes were unaffected by FGF-4. In summary, exogenous FGF-4 increases the rate at which MSC proliferate and has no significant effect on MSC pluripotency.
Farré
Jordi
J
Laboratory of Cell Physiology, Department of Cardiology, Institut Català de Ciencies Cardiovasculars, Hospital de la Santa Creu i Sant Pau, Universitat Autònoma de Barcelona, Sant Antoni Ma Claret 167, 08025 Barcelona, Spain.
Roura
Santiago
S
Prat-Vidal
Cristina
C
Soler-Botija
Carolina
C
Llach
Anna
A
Molina
Cristina E
CE
Hove-Madsen
Leif
L
Cairó
Jordi J
JJ
Gòdia
Francesc
F
Bragós
Ramon
R
Cinca
Juan
J
Bayes-Genis
Antoni
A
D017667:Adipocytes / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D049109:Cell Proliferation; D051519:Fibroblast Growth Factor 4 / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008954:Models, Biological; D032383:Myocytes, Cardiac / Q000378:metabolism; D010012:Osteogenesis; D010641:Phenotype; D013997:Time Factors
C496445:FGF4 protein, human; D051519:Fibroblast Growth Factor 4