title: The flagellar muramidase from the photosynthetic bacterium Rhodobacter sphaeroides.
abstract: We have characterized open reading frame RSP0072, which is located within the flgG operon in Rhodobacter sphaeroides. The amino acid sequence analysis of this gene product showed the presence of a soluble lytic transglycosylase domain. The deletion of the N-terminal region (90 amino acids) of the product of RSP0072 yields a leaky nonmotile phenotype, as determined by swarm assays in soft agar. Electron micrographs revealed the lack of flagella in mutant cells. The purified wild-type protein showed lytic activity on extracts of Micrococcus luteus. In contrast, no lytic activity was observed when the residues E57 or E83 were replaced by alanine. Affinity blotting suggests that the protein encoded by RSP0072 interacts with the flagellar rod-scaffolding protein FlgJ, which lacks the muramidase domain present in FlgJ of many bacteria. We propose that the product of RSP0072 is a flagellar muramidase that is exported to the periplasm via the Sec pathway, where it interacts with FlgJ to open a gap in the peptidoglycan layer for the subsequent penetration of the nascent flagellar structure.
authors.affiliation: Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apdo. Postal 70-243 Cd. Universitaria, México DF 04510, México.
authors.forename: Javier
authors.initials: J
authors.lastname: de la Mora
authors.forename: Teresa
authors.initials: T
authors.lastname: Ballado
authors.forename: Bertha
authors.initials: B
authors.lastname: González-Pedrajo
authors.forename: Laura
authors.initials: L
authors.lastname: Camarena
authors.forename: Georges
authors.initials: G
authors.lastname: Dreyfus
mesh_terms: D005407:Flagella / Q000201:enzymology*; D015964:Gene Expression Regulation, Bacterial; D009113:Muramidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D016366:Open Reading Frames; D009876:Operon; D010788:Photosynthesis / Q000502:physiology; D011485:Protein Binding; D012242:Rhodobacter sphaeroides / Q000201:enzymology*
chemical_list: D009113:Muramidase