Studies on the flavonoid substrates of human UDP-glucuronosyl transferase (UGT) 2B7.
Flavonoids are found in fruits, vegetables, nuts, seeds, herbs, spices, stems and flowers, as well as in tea and red wine. They are prominent components of citrus fruits and other food sources, are consumed regularly with the human diet, and have been shown to have many biological functions, including antioxidant and chelating properties. This study suggests features of the flavonoid structure necessary for it to act as a substrate of human UGT2B7. Generally speaking, flavonol has higher glucuronidation activity than flavones and isoflavones. Differences in C3' position have an important effect on UGT2B7 glucuronidation activity, and the various substituents have different influences on glucuronidation activity. For flavonol, the bulky group at C4' can enhance glucuronidation activity. Increasing the number of hydroxyl groups of flavonoids will increase their glucuronidation activity towards UGT2B7, while conjugation of glycon will weaken the activity, and hydroxyl position can also have an important role in activity. The high glucuronidation efficiency observed with many flavonoids suggests that the contribution of UGT2B7 to the metabolism of flavonoids may be significant. The results suggest that we should not only pay attention to glucuronidation activity, but should also attach importance to the regioselectivity of glucuronidation.
Xie
Shenggu
S
Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
You
Linya
L
Zeng
Su
S
D000818:Animals; D016367:Baculoviridae / Q000378:metabolism; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000378:metabolism*; D020719:Glucuronides / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents; D021241:Spectrometry, Mass, Electrospray Ionization; D018411:Spodoptera; D013329:Structure-Activity Relationship
D005419:Flavonoids; D020719:Glucuronides; D007202:Indicators and Reagents; D014453:Glucuronosyltransferase