Porcine small intestinal submucosa sheets as a scaffold for human bone marrow stem cells.
Native small intestinal submucosa (SIS) sheet was prepared by removal of inside and outside layer of porcine jejunum. The acid treated SIS sheet was also prepared by dipping of native SIS sheet in acetic acid solution. The native or acid treated SIS sheets exhibited elastic and soft property on touch. The surface of native SIS sheet appears to be covered with thin and long collagen fibers entangled into networks. The fibers and fibrils at acid treated SIS sheet disappeared due to the acidic erosion of collagen fiber. The water uptake of acid treated SIS sheet (1300%) was higher than that of the native SIS sheet (500%). The cell morphology and proliferation of human bone marrow stem cells (hBMSCs) on SIS sheet was examined. The hBMSCs on the SIS sheet showed a flattened morphology, while cells in the polyglycolic acid (PGA) mesh showed rounded cell morphology. The cell viability on native or acid treated SIS sheet was higher than that of PGA mesh. The hBMSCs in both native and acid treated SIS sheet were grown at a similar rate. The number of adhering hBMSCs increased with incubation time. Thus, we could confirm that native or acid treated SIS sheet could act as a potential scaffold to enhance the hBMSCs proliferation by providing probably natural environments.
Ahn
Hyun Hee
HH
Fusion Biotechnology Research Center, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong, Daejeon 305-600, Republic of Korea.
Kim
Kyung Sook
KS
Lee
Jung Hwa
JH
Lee
Min Suk
MS
Song
In Bum
IB
Cho
Mi Hee
MH
Shin
Yu Na
YN
Kim
Moon Suk
MS
Khang
Gilson
G
Lee
Hai Bang
HB
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000648:ultrastructure; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000166:cytology* / Q000648:ultrastructure; D007583:Jejunum / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D013234:Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D013552:Swine