[Study on the kinesis of cellular immunity in adults vaccinated with recombinant hepatitis B vaccine].
OBJECTIVE
To evaluate the kinesis of cellular immunity in adults who were vaccinated with yeast recombinant hepatitis B(rHB) vaccine and the correlation between cellular and humoral immune responses induced by the vaccine.


METHODS
Eight adults were vaccinated with rHB vaccine according to 0, 1,2 month schedule. The peripheral blood mononuclear cells(PBMCs) were collected at the 3, 8, 21, 34 and 65 days after the first dose. The high purity of CD4+ and CD8+ T cells obtained by sorting from PBMCs were restimulated with recombinant hepatitis B surface antigens (rHBsAg) or peptides. The spot forming cell (SFC) of IFN-gamma, IL-2 and IL-4 of CD4+ and CD8+ T cells were detected by enzyme-linked immunospot (ELISPOT).


RESULTS
The characteristics of IFN-gamma, IL-2 and IL-4 of CD4+ and CD8+ T cells appeared different after immunization with rHB vaccine. IFN-gamma of CD8+ and CD4+ T cells could be detected early with stable SFC, while the IL-2 and IL-4 of CD4+ T cells appeared late but increased after the second and third dose of vaccination. The positive rate of IL-4 of CD4+ T cells were significantly correlated with the positive rate of anti-HBs, while the SFCs of IL-4 and IL-2 of CD4+ T cells were also significantly related to the titers of anti-FIBs.


CONCLUSIONS
IFN-gamma could be detected early after rHB vaccination in adults, and the positive rates of IL-4 and IL-2 were correlated with that of anti-HBs.
Hu
Zhong-yu
ZY
National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
Zhu
Feng-cai
FC
He
Peng
P
Liu
She-lan
SL
Zhang
Rui
R
Fang
Xing
X
Zhai
Xiang-jun
XJ
Qiu
Shao-hui
SH
Liang
Zheng-lun
ZL
Wang
Hua
H
Li
He-min
HM
Zhuang
Hui
H
D000328:Adult; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D007115:Immunization Schedule; D007371:Interferon-gamma / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D014614:Vaccines, Synthetic / Q000276:immunology
D017325:Hepatitis B Vaccines; D007376:Interleukin-2; D014614:Vaccines, Synthetic; D015847:Interleukin-4; D007371:Interferon-gamma