title: Ameliorating effect of Gardenia jasminoides extract on amyloid beta peptide-induced neuronal cell deficit.
abstract: The brains of Alzheimer's disease (AD) patients are characterized by large deposits of amyloid beta peptide (Abeta). Abeta is known to increase free radical production in nerve cells, leading to cell death that is characterized by lipid peroxidation, free radical formation, protein oxi-dation, and DNA/RNA oxidation. In this study, we selected an extract of Gardenia jasminoides by screening, and investigated its ameliorating effects on Abeta-induced oxidative stress using PC12 cells. The effects of the extract were evaluated using the 2,7 -dichlorofluorescein diacetate (DCF-DA) assay and the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. To find the active component, the ethanol extract was partitioned with hexane, chloroform, and ethyl acetate, respectively, and the active component was purified by silica-gel column chromatography and HPLC. The results suggested that Gardenia jasminoides extract can reduce the cytotoxicity of Abeta in PC 12 cells, possibly by reducing oxidative stress.
authors.affiliation: Department of Food Technology, Korea University, Seoul 136-701, Korea.
authors.forename: Soo Jung
authors.initials: SJ
authors.lastname: Choi
authors.forename: Mi-Jeong
authors.initials: MJ
authors.lastname: Kim
authors.forename: Ho Jin
authors.initials: HJ
authors.lastname: Heo
authors.forename: Bumshik
authors.initials: B
authors.lastname: Hong
authors.forename: Hong Yon
authors.initials: HY
authors.lastname: Cho
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Kim
authors.forename: Hye Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Seung-Taik
authors.initials: ST
authors.lastname: Lim
authors.forename: Woo Jin
authors.initials: WJ
authors.lastname: Jun
authors.forename: Eun-Ki
authors.initials: EK
authors.lastname: Kim
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Shin
mesh_terms: D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001362:Avoidance Learning / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D032062:Gardenia / Q000737:chemistry*; D008297:Male; D008568:Memory / Q000187:drug effects; D051379:Mice; D009474:Neurons / Q000187:drug effects; D016716:PC12 Cells; D019308:Palmitic Acid / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D013037:Spatial Behavior / Q000187:drug effects
chemical_list: D016229:Amyloid beta-Peptides; D000975:Antioxidants; D010936:Plant Extracts; D019308:Palmitic Acid