Effects of different surfactants on motility and DNA integrity of brown trout (Salmo trutta fario) and common carp (Cyprinus carpio) spermatozoa.
In this study we evaluated effects of surfactants on motility parameters and DNA integrity of spermatozoa of freshwater teleost fish. Common carp (Cyprinus carpio) and brown trout (Salmo trutta fario) spermatozoa were exposed to either sodium dodecyl sulphate (SDS, anionic surfactant) or octoxynol 9 ( Triton X-100, nonionic surfactant). Both surfactants added at activation caused a decrease in sperm motility characteristics measured by computer-assisted sperm analysis (CASA). Intraspecific differences in speed and trajectory of movement were detected. Triton X-100 and SDS when added to non activated sperm were also effective in the decrease of sperm motility and caused an increase of DNA fragmentation. Our results suggest that not only sperm motility apparatus but also DNA are targets for surfactant action. Therefore any exposure of spermatozoa to surfactants, in aquaculture conditions or natural environment, would have a negative impact on fish reproduction.
Dietrich
Grzegorz J
GJ
Semen Biology Group, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
Zabowska
Marta
M
Wojtczak
Mariola
M
Słowińska
Mariola
M
Kucharczyk
Dariusz
D
Ciereszko
Andrzej
A
D000818:Animals; D002347:Carps / Q000502:physiology*; D053938:DNA Fragmentation; D008297:Male; D017830:Octoxynol / Q000494:pharmacology; D012661:Semen / Q000502:physiology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology*; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013501:Surface-Active Agents / Q000494:pharmacology*; D014337:Trout / Q000502:physiology*
D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D017830:Octoxynol