title: Characterization of Bordetella pertussis clinical isolates that do not express the tracheal colonization factor.
abstract: Bordetella pertussis, the causative agent of whooping cough, has remained endemic and there is a resurgence in some countries despite vaccination. Bordetella pertussis produces a wide range of virulence factors which are assumed to play an important role in infection and transmission, including tracheal colonization factor (TcfA). Here we show that clinical isolates belonging to distinct lineages may lose their ability to produce TcfA. Irreversible and reversible loss occurred, respectively, by recombination between repeats leading to deletion of the tcfA gene and by mutations in a polymorphic G-track. These phenomena may reflect adaptation to distinct niches.
authors.affiliation: Laboratory for Infectious Diseases and Screening, National Institute for Public Health and the Environment, Bilthoven, The Netherlands.
authors.forename: Marjolein
authors.initials: M
authors.lastname: van Gent
authors.forename: Dénis
authors.initials: D
authors.lastname: Pierard
authors.forename: Sabine
authors.initials: S
authors.lastname: Lauwers
authors.forename: Han G J
authors.initials: HG
authors.lastname: van der Heide
authors.forename: Audrey J
authors.initials: AJ
authors.lastname: King
authors.forename: Frits R
authors.initials: FR
authors.lastname: Mooi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D001886:Bordetella pertussis / Q000472:pathogenicity*; D006801:Humans; D009154:Mutation; D010566:Virulence Factors, Bordetella / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D010566:Virulence Factors, Bordetella; C096309:tracheal colonization factor, Bordetella pertussis