title: Comparative analysis of skin sensitization potency of acrylates (methyl acrylate, ethyl acrylate, butyl acrylate, and ethylhexyl acrylate) using the local lymph node assay.
abstract: There are currently available no systematic experimental data on the skin sensitizing properties of acrylates that are of relevance in occupational settings. Limited information from previous guinea-pig tests or from the local lymph node assay (LLNA) is available; however, these data are incomplete and somewhat contradictory. For those reasons, we have examined in the LLNA 4 acrylates: butyl acrylate (BA), ethyl acrylate (EA), methyl acrylate (MA), and ethylhexyl acrylate (EHA). The LLNA data indicated that all 4 compounds have some potential to cause skin sensitization. In addition, the relative potencies of these acrylates were measured by derivation from LLNA dose-response analyses of EC3 values (the effective concentration of chemical required to induce a threefold increase in proliferation of draining lymph node cells compared with control values). On the basis of 1 scheme for the categorization of skin sensitization potency, BA, EA, and MA were each classified as weak sensitizers. Using the same scheme, EHA was considered a moderate sensitizer. However, it must be emphasized that the EC3 value for this chemical of 9.7% is on the borderline between moderate (<10%) and weak (>10%) categories. Thus, the judicious view is that all 4 chemicals possess relatively weak skin sensitizing potential.
authors.affiliation: Immunology, Syngenta Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, SK1 4TJ, UK.
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Dearman
authors.forename: Catherine J
authors.initials: CJ
authors.lastname: Betts
authors.forename: Craig
authors.initials: C
authors.lastname: Farr
authors.forename: James
authors.initials: J
authors.lastname: McLaughlin
authors.forename: Nancy
authors.initials: N
authors.lastname: Berdasco
authors.forename: Karin
authors.initials: K
authors.lastname: Wiench
authors.forename: Ian
authors.initials: I
authors.lastname: Kimber
mesh_terms: D000179:Acrylates / Q000633:toxicity*; D000485:Allergens / Q000633:toxicity*; D000818:Animals; D049109:Cell Proliferation; D017449:Dermatitis, Allergic Contact / Q000209:etiology*; D004195:Disease Models, Animal; D005260:Female; D023441:Local Lymph Node Assay; D008198:Lymph Nodes / Q000473:pathology; D051379:Mice; D008808:Mice, Inbred CBA; D012882:Skin Tests
chemical_list: D000179:Acrylates; D000485:Allergens