title: Cytoplasmic cell fusion: Stembrid technology for reprogramming pluripotentiality.
abstract: Recent progress in somatic cell nuclear transfer (SCNT) provides the evidence for the presence of reprogramming factors in human embryonic stem cells (hESC). Hybrid hESC with donor human somatic nuclei have been established, but the resulting hybrid hESC contained DNA originating from both hESC and donor somatic cells. There is still no method to completely replace the hESC nuclei by the nuclei of somatic cells and to obtain the pure colonies of hESC with donor genotype. We present here the original technology, which is based on enucleation of h ESC and their fusion with the adult somatic cells, resulting in the establishment of individual-specific hESC with the genotype of the donor somatic cells. The resulting constructs was demonstrated to have the "stemness" of hESC and the genotype of the donor somatic cells. So this "Stembrid technology," may be used for the construction of patient-specific hESC.
authors.affiliation: Reproductive Genetics Institute, 2825 N Halsted St, Chicago, IL 60657, USA.
authors.forename: Yury
authors.initials: Y
authors.lastname: Verlinsky
authors.forename: Nick
authors.initials: N
authors.lastname: Strelchenko
authors.forename: Artem
authors.initials: A
authors.lastname: Shkumatov
authors.forename: Valeri
authors.initials: V
authors.lastname: Kukharenko
authors.forename: Oleg
authors.initials: O
authors.lastname: Verlinsky
authors.forename: Anver
authors.initials: A
authors.lastname: Kuliev
mesh_terms: D002459:Cell Fusion; D065150:Cellular Reprogramming; D053595:Embryonic Stem Cells / Q000502:physiology; D006801:Humans; D006822:Hybrid Cells / Q000502:physiology*; D039904:Pluripotent Stem Cells / Q000502:physiology*