[Association of variations in MyoD family of genes with body measurement traits in goat].
Single nucleotide polymorphisms (SNPs) of hircine myf-5, myf-6 genes and 5'flanking region of the myoD gene (myoD 5') were analyzed by PCR-SSCP in Boer goat and Xuhuai white goat. Three genotypes were found at the myf-5 locus of Xuhuai white goat, denoted AA, AB and BB, while all of Boer goats were of AA genotype. Only AA and AB genotypes were found at myf-6 and myoD 5' loci. The effects of genotypes of myf-5, myf-6 and myoD 5'flanking region loci on body measurement traits were estimated. The results showed that genotypes at the myf-5 locus were significantly associated with hucklebone width and hucklebone width index in Xuhuai white goat (P<0.05). Genotypes at the myf-6 locus were significantly associated with withers heights and hucklebone width index in Boer goat (P<0.05), but no significant associations were found for any traits in Xuhuai white goat. The myoD 5' locus was not significantly associated with any traits in both types of goat.
Zhang
Hai-Jun
HJ
Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China. haijunzh1981@126.com
Chen
Hong
H
Fang
Xing-Tang
XT
Zhang
Run-Feng
RF
Bao
Bin
B
Gao
Xue-Yuan
XY
Shao
Ru-Ying
RY
D000818:Animals; D049628:Body Size / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006041:Goats / Q000235:genetics*; D017570:MyoD Protein / Q000235:genetics*; D051787:Myogenic Regulatory Factor 5 / Q000235:genetics; D018006:Myogenic Regulatory Factors / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational
D017570:MyoD Protein; D051787:Myogenic Regulatory Factor 5; D018006:Myogenic Regulatory Factors; C063403:myogenic factor 6