Adsorption kinetics of c-Fos and c-Jun to air-water interfaces.
The kinetics of adsorption to air-water interfaces of the biomembrane active transcription factors c-Fos, c-Jun and their mixtures is investigated. The adsorption process shows three distinct stages: a lag time, a fast pseudo zero-order stage, and a halting stage. The initial stage determines the course of the process, which is concentration dependent until the end of the fast stage. We show that c-Fos has faster adsorption kinetics than c-Jun over all three stages and that the interaction between both proteins is apparent in the adsorption profiles of the mixtures. Protein molecular reorganization at the interface determines the transition to the final adsorption stage of the pure proteins as well as that of the mixtures.
Del Boca
Maximiliano
M
Centro de Investigaciones en Química Biológica de Córdoba (UNC-CONICET), Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, X5000HUA, Córdoba, Argentina.
Nobre
Thatyane Morimoto
TM
Zaniquelli
Maria Elisabete Darbello
ME
Maggio
Bruno
B
Borioli
Graciela A
GA
D000327:Adsorption; D000388:Air; D006801:Humans; D007700:Kinetics; D016760:Proto-Oncogene Proteins c-fos / Q000737:chemistry* / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D014867:Water / Q000737:chemistry* / Q000378:metabolism
D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D014867:Water