Real-time PCR genotyping of aldehyde dehydrogenase-2 using displacing probes.
OBJECTIVE
This study aimed to develop and validate a rapid, accurate method for aldehyde dehydrogenase-2 gene (ALDH2) genotyping.


METHODS
We reported a single-tube, real-time PCR method for ALDH2 allele detection using two displacing probes.


RESULTS
The genotyping results of 136 human genomic DNA samples indicated 100% concordance between real-time PCR method and the PCR-RFLP analysis.


CONCLUSIONS
The developed method could be used for routine clinical testing and high throughput genetic screening of ALDH2.
Gu
Yingying
Y
Molecular Diagnostics Laboratory, Department of Biomedical Sciences, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China.
Li
Qingge
Q
D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D005838:Genotype; D006801:Humans; D015345:Oligonucleotide Probes / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results
D015345:Oligonucleotide Probes; C096127:ALDH2 protein, human; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial