title: Proteome of murine jejunal brush border membrane vesicles.
abstract: The first detailed description of the proteome of the mouse jejunal brush border membrane vesicle is presented here. This was obtained by a combination of purification via divalent (Mg2+) cation precipitation starting with isolated cells plus strong cation exchange chromatography LC-MS/MS. Five-hundred seventy proteins were identified including 45 transport proteins. Among the latter, 18 had either not been identified in the intestine in the past or there was a single unconfirmed report of their presence. Validation was accomplished by a combination of immunoblotting and immunofluorescence using mouse jejunum and previously described antibodies. The validated BB proteins were aquaporin 7, Glut 9b, Na+I- symporter (NIS), and non-gastric H+/K+-ATPase. This study helps to further define the brush border membrane vesicle, a preparation which has been widely used to identify transport function of the small intestine.
authors.forename: Mark
authors.initials: M
authors.lastname: Donowitz
authors.forename: Siddharth
authors.initials: S
authors.lastname: Singh
authors.forename: Farah F
authors.initials: FF
authors.lastname: Salahuddin
authors.forename: Boris M
authors.initials: BM
authors.lastname: Hogema
authors.forename: Yueping
authors.initials: Y
authors.lastname: Chen
authors.forename: Marjan
authors.initials: M
authors.lastname: Gucek
authors.forename: Robert N
authors.initials: RN
authors.lastname: Cole
authors.forename: Amy
authors.initials: A
authors.lastname: Ham
authors.forename: Nicholas C
authors.initials: NC
authors.lastname: Zachos
authors.forename: Olga
authors.initials: O
authors.lastname: Kovbasnjuk
authors.forename: Lynne A
authors.initials: LA
authors.lastname: Lapierre
authors.forename: Nellie
authors.initials: N
authors.lastname: Broere
authors.forename: James
authors.initials: J
authors.lastname: Goldenring
authors.forename: Hugo
authors.initials: H
authors.lastname: deJonge
authors.forename: Xuhang
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D007413:Intestinal Mucosa / Q000378:metabolism; D007583:Jejunum / Q000378:metabolism*; D008297:Male; D051379:Mice; D008871:Microvilli / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome