[Effect of isoflavones in regulating the transcription of target genes through estrogen receptors].
OBJECTIVE
To observe the transcriptional regulation of the two isoflavones genistein and daidzein on target genes.


METHODS
In this study, we used ERalpha or ERbeta over-expressing Hela cells to observe the transcriptional regulation of genistein and daidzein on ERE reporter gene with calcium-phosphate method, and furthermore observing the effects of phytoestrogen antagonist ICI 182780 on their activation.


RESULTS
Our results showed that both genistein and daidzein could activate ERE receptor gene through ERalpha and ERbeta, and these effects could be blocked by ICI 182780.


CONCLUSIONS
Both genistein and daidzein can mimic estrogen's effect to activate the transcription of target genes through binding to the ERs.
Meng
Qing-shu
QS
Department of Physiology, College of Basic Medical Science, Second Military Medical University, Shanghai 200433, China.
Zhu
Xiao-yan
XY
Tang
Xiao-lu
XL
Ma
Bei
B
Ni
Xin
X
D047628:Estrogen Receptor alpha / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D048789:Phytoestrogens / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D007529:Isoflavones; D048789:Phytoestrogens; C004742:daidzein; D019833:Genistein