title: Demonstration of in vivo expression of a hypothetical open reading frame (ORF-14) encoded by the RD1 region of Mycobacterium tuberculosis.
abstract: Previously we identified a novel antigenic open reading frame (ORF), designated as ORF-14, on the RD1 region of Mycobacterium tuberculosis that was not originally predicted by Mahairas or by annotation of the M. tuberculosis H37 Rv genome. Here we show that anti-ORF-14 antibodies either from mice immunized with recombinant ORF-14 protein or isolated from serum samples from tuberculosis patients, react with a protein in culture filtrate but not in cytoplasmic or cell wall fractions from M. tuberculosis. Our data indicate that the ORF-14 protein is expressed as a secreted protein, representing one more secreted protein antigen not previously identified by genomics.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait. hanady@hsc.edu.kw
authors.forename: H A
authors.initials: HA
authors.lastname: Amoudy
authors.forename: S
authors.initials: S
authors.lastname: Ahmad
authors.forename: J E
authors.initials: JE
authors.lastname: Thole
authors.forename: A S
authors.initials: AS
authors.lastname: Mustafa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D015153:Blotting, Western; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000276:immunology; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D011994:Recombinant Proteins