title: RGD peptides grafting onto poly(ethylene terephthalate) with well controlled densities.
abstract: The aim of this study was to graft RGD peptides with well controlled densities onto poly(ethylene terephthalate) (PET) film surfaces. Biomimetic modifications were performed by means of a four-step reaction procedure: surface modification in order to create -COOH groups onto polymer surface, coupling agent grafting and finally immobilization of peptides. The originality of this work is to evaluate several grafted densities peptides. Toluidine blue and high-resolution mu-imager (using [(3)H]-Lys) were used to evaluate densities. Moreover, mu-imager has exhibited the stability of peptides grafted onto the surface when treated under harsh conditions. Benefits of the as-proposed method were related to the different concentrations of peptides grafted onto the surface as well as the capacity of RGD peptide to interact with integrin receptors.
authors.affiliation: INSERM U577, Bordeaux, F-33076, France. celine.chollet@bordeaux.inserm.fr
authors.forename: C
authors.initials: C
authors.lastname: Chollet
authors.forename: C
authors.initials: C
authors.lastname: Chanseau
authors.forename: B
authors.initials: B
authors.lastname: Brouillaud
authors.forename: M C
authors.initials: MC
authors.lastname: Durrieu
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D032701:Biomimetics; D008239:Lysine / Q000737:chemistry; D008567:Membranes, Artificial; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000528:radiation effects; D011093:Polyethylene Terephthalates; D013499:Surface Properties; D014048:Tolonium Chloride / Q000737:chemistry; D014316:Tritium / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D009842:Oligopeptides; D011093:Polyethylene Terephthalates; D014316:Tritium; D014048:Tolonium Chloride; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid; D008239:Lysine