title: Experimental evaluation of angularly variable fiber geometry for targeting depth-resolved reflectance from layered epithelial tissue phantoms.
abstract: The present study focuses on enhancing the sensitivity and specificity of spectral diagnosis in a stratified architecture that models human cervical epithelia by experimentally demonstrating the efficacy of using angularly variable fiber geometry to achieve the desired layer selection and probing depths. The morphological and biochemical features of epithelial tissue vary in accordance with tissue depths; consequently, the accuracy of spectroscopic diagnosis of epithelial dysplasia may be enhanced by probing the optical properties of this tissue. In the case of cellular dysplasia, layer-specific changes in tissue optical properties may be optimally determined by reflectance spectroscopy when specifically coupled with angularly variable fiber geometry. This study addresses the utility of using such angularly variable fiber geometry for resolving spatially specific spectra of a two-layer epithelial tissue phantom. Spectral sensitivity to the scattering particles embedded in the epithelial phantom layer is shown to significantly improve as the obliquity of the collection fibers increases from 0 to 40 deg. Conversely, the orthogonal fibers are found to be more sensitive to changes in the stromal phantom layer.
authors.affiliation: Rice University, Department of Bioengineering, Houston, Texas 77251-1892, USA.
authors.forename: Adrien
authors.initials: A
authors.lastname: Wang
authors.forename: Vengadesan
authors.initials: V
authors.lastname: Nammalavar
authors.forename: Rebekah
authors.initials: R
authors.lastname: Drezek
mesh_terms: D016880:Anisotropy; D002584:Cervix Uteri / Q000166:cytology* / Q000502:physiology*; D004848:Epithelium / Q000502:physiology* / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D019047:Phantoms, Imaging; D010783:Photometry / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*