Apoptosis and proliferation differences between CD34+ and CD34- leukemic subpopulations in childhood acute leukemia.
In view of the clinical and biological significance of leukemic heterogeneity we studied the efficacy of spontaneous apoptosis and cell cycle distribution in CD34+ and CD34 - leukemic subpopulations. Acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML) leukemic samples with CD34 heterogeneous expression were separated into CD34+ and CD34 - fractions using fluorescence activated cell sorting. Cell cycle distribution, and apoptosis of the sorted subpopulations were estimated. CD34+ leukemic subpopulations had lower ability to apoptosis than that of CD34 - fractions in 6 out of 8 ALL samples and in 4 out of 5 AML samples. CD34+ fractions showed a higher percentage of proliferating cells compared to CD34 - cells in T-lineage ALL. These differences may lead to a more resistant phenotype of one of the subpopulations and reappearance this population in relapse.
Shman
Tatsiana V
TV
Belarusian Research Center for Pediatric Oncology and Hematology, Minsk, Belarus. t_shman@yahoo.com
Savitski
Valery P
VP
Fedasenka
Uladzimir U
UU
Aleinikova
Olga V
OV
D018952:Antigens, CD34; D017209:Apoptosis; D002453:Cell Cycle; D049109:Cell Proliferation; D002648:Child; D005434:Flow Cytometry; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology*
D018952:Antigens, CD34