Mapping of Ki-67 protein distribution on whole organ serial sections of the larynx.
CONCLUSIONS
Immunostaining of whole organ serial sections of the larynx is feasible and will allow analysis of cellular alterations in the undisturbed anatomical context of whole organ serial sections of the larynx.


OBJECTIVE
Whole organ serial sections of the larynx have to date been used for conventional macroscopic evaluation of laryngeal tissues. The aim of this study was to establish a protocol for immunohistochemistry of whole organ sections of the larynx.


METHODS
Five laryngectomy specimens were obtained during surgery for advanced laryngeal carcinoma. Using a novel method for paraffin embedding, we chose the proliferation marker Ki-67 antigen as a model target for immunoreactivity on serial sections.


RESULTS
We were able to produce whole organ serial sections that could then be immunostained for Ki-67. A complete mapping of proliferating cells throughout the tumour, at the tumour front and in skip lesions was subsequently obtained.
Wittekindt
Claus
C
Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Jena, Jena, Germany.
Sittel
Christian
C
Greiss
Julian
J
Drebber
Uta
U
Eckel
Hans Edmund
HE
Preuss
Simon Florian
SF
D000368:Aged; D002455:Cell Division / Q000502:physiology; D005260:Female; D006652:Histological Techniques; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen / Q000032:analysis*; D007822:Laryngeal Neoplasms / Q000473:pathology* / Q000601:surgery; D007825:Laryngectomy; D007830:Larynx / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging
D019394:Ki-67 Antigen