Isoproterenol hydrochloride augments collagen proliferation and ATPase activity in mice soleus and EDL muscles.
Aim of this study is to analyze the effect of chronic administration of beta agonist isoproterenol hydrochloride (60 mg kg(-1) day(-1); 30 days) on soleus (a slow type) and extensor digitorum longus (EDL, a fast type) muscles in young mice. Isoproterenol resulted in significant increase in muscle weight to whole body weight ratio with no increase in hypertrophy index in soleus muscle. A significant increase in noncontractile protein collagen is also observed in both muscles but more prominent in soleus muscle. Collagen proliferation is also analyzed on sodium dodecyle sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of pepsin soluble and Cyanogen Bromide (CN Br) treated pepsin insoluble collagen. Isoproterenol remolded the myofibrillar proteins in both muscles but significant increase in myofibrillar ATPase activity occurred only in soleus muscle. It is concluded that growth stimulatory effect of isoproterenol hydrochloride is more prominent in soleus than FDL muscle. Isoproterenol augmented the proliferation of non-contractile protein collagen in soleus and EDL muscles. The transformation in myofibrillar proteins caused by isoproterenol might lead to an enhancement of contractile performance.
Kumar
R
R
Department of Biosciences, Himachal Pradesh University, Summer Hill, Shimla-171005, India.
Sharma
S
S
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D003094:Collagen / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D051379:Mice; D024510:Muscle Development / Q000187:drug effects*; D018656:Muscle Fibers, Fast-Twitch / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D018657:Muscle Fibers, Slow-Twitch / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D009210:Myofibrils / Q000187:drug effects / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects
D000318:Adrenergic beta-Agonists; D003094:Collagen; D017301:Ca(2+) Mg(2+)-ATPase; D007545:Isoproterenol