Increased caloric intake on a fat-rich diet: role of ovarian steroids and galanin in the medial preoptic and paraventricular nuclei and anterior pituitary of female rats.
Previous studies in male rats have demonstrated that the orexigenic peptide galanin (GAL), in neurones of the anterior parvocellular region of the paraventricular nucleus (aPVN) projecting to the median eminence (ME), is stimulated by consumption of a high-fat diet and may have a role in the hyperphagia induced by fat. In addition to confirming this relationship in female rats and distinguishing the aPVN-ME from other hypothalamic areas, the present study identified two additional extra-hypothalamic sites where GAL is stimulated by dietary fat in females but not males. These sites were the medial preoptic nucleus (MPN), located immediately rostral to the aPVN, and the anterior pituitary (AP). The involvement of ovarian steroids, oestradiol (E(2)) and progesterone (PROG), in this phenomenon was suggested by an observed increase in circulating levels of these hormones and GAL in MPN and AP with fat consumption and an attenuation of this effect on GAL in ovariectomised (OVX) rats. Furthermore, in the same four areas affected by dietary fat, levels of GAL mRNA and peptide immunoreactivity were stimulated by E(2) and further by PROG replacement in E(2)-primed OVX rats and were higher in females compared to males. Because both GAL and PROG stimulate feeding, their increase on a fat-rich diet may have functional consequences in females, possibly contributing to the increased caloric intake induced by dietary fat. This is supported by the findings that PROG administration in E(2)-primed OVX rats reverses the inhibitory effect of E(2) on total caloric intake while increasing voluntary fat ingestion, and that female rats with higher GAL exhibit increased preference for fat compared to males. Thus, ovarian steroids may function together with GAL in a neurocircuit, involving the MPN, aPVN, ME and AP, which coordinate feeding behaviour with reproductive function to promote consumption of a fat-rich diet at times of increased energy demand.
Leibowitz
S F
SF
The Rockefeller University, New York, NY 10021, USA. leibow@rockefeller.edu
Akabayashi
A
A
Wang
J
J
Alexander
J T
JT
Dourmashkin
J T
JT
Chang
G-Q
GQ
D000818:Animals; D001069:Appetite Regulation / Q000502:physiology*; D004041:Dietary Fats / Q000378:metabolism; D002149:Energy Intake / Q000502:physiology*; D004958:Estradiol / Q000097:blood; D005247:Feeding Behavior / Q000502:physiology; D005260:Female; D005518:Food Preferences / Q000502:physiology; D019004:Galanin / Q000235:genetics / Q000502:physiology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009434:Neural Pathways / Q000502:physiology; D010286:Paraventricular Hypothalamic Nucleus / Q000502:physiology*; D010903:Pituitary Gland, Anterior / Q000502:physiology*; D011301:Preoptic Area / Q000502:physiology*; D011374:Progesterone / Q000097:blood; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012098:Reproduction / Q000502:physiology; D012737:Sex Factors
D004041:Dietary Fats; D012333:RNA, Messenger; D011374:Progesterone; D004958:Estradiol; D019004:Galanin