Gene expression of secretory carbonic anhydrase isozymes in striated ducts of canine salivary glands using laser microdissection system.
To clarify whether striated duct cells in canine salivary glands synthesize secretory carbonic anhydrase (CA-VI), as is the case with serous acinar cells, the present study utilized laser microdissection to harvest striated duct cells from canine parotid and submandibular glands, and total RNA extracted from these cells was then amplified by reverse transcription-polymerase chain reaction to assess CA-VI gene expression. The results confirmed the local expression of CA-VI mRNA in striated duct cells. This clarified that, in canine salivary glands, CA-VI is synthesized in not only serous acinar cells, but also striated duct cells.
Ichihara
N
N
Laboratory of Anatomy I, Azabu University School of Veterinary Medicine, Fuchinobe 1-17-71, Sagamihara, Kanagawa 229-8501, Japan. ichihara@azabu-u.ac.jp
Tsukamoto
A
A
Kasuya
T
T
Shibata
S
S
Nishita
T
T
Murakami
M
M
Amasaki
H
H
Asari
M
M
D000818:Animals; D002256:Carbonic Anhydrases / Q000032:analysis / Q000378:metabolism*; D004285:Dogs / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D007150:Immunohistochemistry / Q000662:veterinary; D007527:Isoenzymes; D007834:Lasers; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012469:Salivary Glands / Q000166:cytology / Q000201:enzymology*
D007527:Isoenzymes; D002256:Carbonic Anhydrases; C477051:carbonic anhydrase VI