Synthesis of nucleotide analogues by a promiscuous phosphoribosyltransferase.
An Escherichia coli strain overexpressing a mutant variant of a phosphoribosyl transferase was developed as a catalyst for the efficient preparation of a range of purine nucleotide analogues. This system offers an efficient and rapid method for nucleotide analogue synthesis with 100% beta-selectivity, providing analytically pure product in a single purification step.
Scism
Robert A
RA
Department of Chemistry, Vanderbilt University, 7300 Stevenson Center Lane, Nashville, Tennessee 37235, USA.
Stec
Donald F
DF
Bachmann
Brian O
BO
D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D019906:Nuclear Magnetic Resonance, Biomolecular; D011684:Purine Nucleosides / Q000138:chemical synthesis* / Q000737:chemistry
D011684:Purine Nucleosides; D007041:Hypoxanthine Phosphoribosyltransferase