Oral administration of the extract from Hatakeshimeji (Lyophyllum decastes sing.) mushroom inhibits the development of atopic dermatitis-like skin lesions in NC/Nga mice.
We examined whether the extract from Hatakeshimeji (Lyophyllum decastes, LD) mushrooms suppresses the development of atopic dermatitis (AD)-like skin lesions induced by repeated application of picryl chloride (PiCl) in NC/Nga mice. Oral administration of LD extract to NC/Nga mice inhibited the development of AD-like skin lesions based on lower total skin severity scores and serum immunoglobulin E (IgE) levels. Splenic lymphocytes were stimulated with the T cell mitogen concanavalin A, and secretion of a Th1 cytokine (IFN-gamma) and a Th2 cytokine (IL-4) was determined by ELISA. IFN-gamma production was not inhibited by treatment with LD extract. On the other hand, IL-4 production was significantly decreased by treatment with LD extract. These results suggest that LD extract exerts anti-allergic actions by suppressing the serum IgE and Th2-type immune responses.
Ukawa
Yuuichi
Y
Oji Forest & Products Co., Ltd., 1-1-1 Shinkiba, Koutou-ku, Tokyo 136-0082, Japan. ukawa@vc.tnc.ne.jp
Izumi
Yoshiya
Y
Ohbuchi
Takayuki
T
Takahashi
Tetsunari
T
Ikemizu
Shoichi
S
Kojima
Yasushi
Y
D000284:Administration, Oral; D000363:Agaricales / Q000737:chemistry; D000818:Animals; D003208:Concanavalin A; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000517:prevention & control*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D006632:Histamine / Q000097:blood / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood / Q000187:drug effects; D007371:Interferon-gamma / Q000187:drug effects / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008517:Phytotherapy / Q000379:methods; D010853:Picryl Chloride; D010936:Plant Extracts / Q000008:administration & dosage; D012720:Severity of Illness Index; D012867:Skin / Q000187:drug effects* / Q000276:immunology; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013997:Time Factors
D010936:Plant Extracts; D003208:Concanavalin A; D015847:Interleukin-4; D007073:Immunoglobulin E; D006632:Histamine; D007371:Interferon-gamma; D010853:Picryl Chloride