Rapid determination of viral RNA sequences in mosquitoes collected in the field.
A method for rapid determination of viral RNA sequences (RDV) was applied to homogenates of Aedes aegypti collected in Thailand in an area in which dengue fever (dengue hemorrhagic fever) is endemic, using the mosquito cell line C6/36. Nucleic acid sequences of dengue virus type 4 and cell fusing agent virus were detected. This RDV method has the potential to become a standard method for detection of both known and newly emerging, unknown mosquito-borne viruses.
Kihara
Yuki
Y
Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.
Satho
Tomomitsu
T
Eshita
Yuki
Y
Sakai
Kouji
K
Kotaki
Akira
A
Takasaki
Tomohiko
T
Rongsriyam
Yupha
Y
Komalamisra
Narumon
N
Srisawat
Raweewan
R
Lapcharoen
Parichat
P
Sumroiphon
Suchada
S
Iwanaga
Shiroh
S
Ushijima
Hiroshi
H
Endoh
Daiji
D
Miyata
Takeshi
T
Sakata
Akira
A
Kashige
Nobuhiro
N
Miake
Fumio
F
Fukushi
Shuetsu
S
Saijo
Masayuki
M
Kurane
Ichiro
I
Morikawa
Shigeru
S
Mizutani
Tetsuya
T
D000330:Aedes / Q000821:virology*; D000818:Animals; D002460:Cell Line; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D019595:Severe Dengue / Q000821:virology; D013785:Thailand
D012367:RNA, Viral