The emerging picture of the mouse mammary stem cell.
The isolation and characterisation of mammary stem cells is an important step towards elucidating the hierarchy of epithelial cell development in the mammary gland and identifying cells that are targets of breast carcinogenesis. Mammary stem cells have recently been prospectively isolated through the identification of specific cell surface markers and in vivo transplantation into cleared fat pads. These cells were demonstrated to reconstitute an entire mammary gland comprising all mature epithelial cell types and to be capable of self-renewal on serial transplantation, thus possessing the defining features of stem cells. Notably, mouse mammary stem cells were found to share the hallmark properties of the basal subtype of breast cancer. This review will summarize the strategy used in the identification of mouse mammary stem cells and their characterisation.
Vaillant
François
F
Victorian Breast Cancer Research Consortium Laboratory, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3050, Australia.
Asselin-Labat
Marie-Liesse
ML
Shackleton
Mark
M
Lindeman
Geoffrey J
GJ
Visvader
Jane E
JE
D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D002454:Cell Differentiation / Q000502:physiology; D002469:Cell Separation / Q000379:methods; D004847:Epithelial Cells / Q000166:cytology* / Q000502:physiology*; D005260:Female; D006801:Humans; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000502:physiology*; D042361:Mammary Glands, Human / Q000166:cytology / Q000502:physiology; D015674:Mammary Neoplasms, Animal / Q000378:metabolism / Q000473:pathology; D051379:Mice; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*
D000943:Antigens, Differentiation