Diploidized eggs reprogram adult somatic cell nuclei to pluripotency in nuclear transfer in medaka fish (Oryzias latipes).
Reprogramming of adult somatic cell nuclei to pluripotency has been unsuccessful in non-mammalian animals, primarily because of chromosomal aberrations in nuclear transplants, which are considered to be caused by asynchrony between the cell cycles of the recipient egg and donor nucleus. In order to normalize the chromosomal status, we used diploidized eggs by retention of second polar body release, instead of enucleated eggs, as recipients in nuclear transfer of primary culture cells from the caudal fin of adult green fluorescent protein gene (GFP) transgenic medaka fish (Oryzias latipes). We found that 2.7% of the reconstructed embryos grew into adults that expressed GFP in various tissues in the same pattern as in the donor fish. Moreover, these fish were diploid, fertile and capable of passing the marker gene to the next generation in Mendelian fashion. We hesitate to call these fish 'clones' because we used non-enucleated eggs as recipients; in effect, they may be chimeras consisting of cells derived from diploid recipient nuclei and donor nuclei. In either case, fish adult somatic cell nuclei were reprogrammed to pluripotency and differentiated into a variety of cell types including germ cells via the use of diploidized recipient eggs.
Bubenshchikova
Ekaterina
E
Laboratory of Freshwater Fish Stocks, Bioscience and Biotechnology Center, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Kaftanovskaya
Elena
E
Motosugi
Nami
N
Fujimoto
Takafumi
T
Arai
Katsutoshi
K
Kinoshita
Masato
M
Hashimoto
Hisashi
H
Ozato
Kenjiro
K
Wakamatsu
Yuko
Y
D000818:Animals; D002467:Cell Nucleus / Q000235:genetics* / Q000378:metabolism; D004171:Diploidy; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D053652:Nuclear Transfer Techniques; D009990:Oryzias / Q000235:genetics*; D010063:Ovum / Q000166:cytology / Q000378:metabolism*; D011003:Ploidies; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism
D049452:Green Fluorescent Proteins