Suppression of reactive oxygen species by glyceraldehyde-3-phosphate dehydrogenase.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a classical glycolytic enzyme, is involved in cellular energy production and has important housekeeping functions. In this report, we show that a GAPDH from Arabidopsis, GAPDHa, has a novel function involved in H(2)O(2)-mediated cell death in yeast and Arabidopsis protoplasts. GAPDHa was cloned along with other plant genes that suppress Bax-induced cell death in yeast. Flow cytometry analyses with dihydrorhodamine 123 indicated that H(2)O(2) production mediated by Bax expression in yeast cells was greatly reduced when Bax was coexpressed with GAPDHa. In plants, GAPDHa transcript levels were greatly increased by H(2)O(2) treatment. Furthermore, transformation of GAPDHa into Arabidopsis protoplasts strongly suppressed heat shock-induced H(2)O(2) production and cell death. Together, our results indicate that GAPDH controls generation of H(2)O(2) by Bax and heat shock, which in turn suppresses cell death in yeast and plant cells.
Baek
Dongwon
D
Division of Applied Life Science (BK21 program) and Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Jin
Yinhua
Y
Jeong
Jae Cheol
JC
Lee
Hyo-Jung
HJ
Moon
Haejeong
H
Lee
Jiyoung
J
Shin
Dongjin
D
Kang
Chang Ho
CH
Kim
Doh Hoon
DH
Nam
Jaesung
J
Lee
Sang Yeol
SY
Yun
Dae-Jin
DJ
D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology / Q000235:genetics; D016923:Cell Death; D015971:Gene Expression Regulation, Enzymologic; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress; D011523:Protoplasts / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases