title: DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication.
abstract: DDX3, a DEAD-box RNA helicase, binds to the hepatitis C virus (HCV) core protein. However, the role(s) of DDX3 in HCV replication is still not understood. Here we demonstrate that the accumulation of both genome-length HCV RNA (HCV-O, genotype 1b) and its replicon RNA were significantly suppressed in HuH-7-derived cells expressing short hairpin RNA targeted to DDX3 by lentivirus vector transduction. As well, RNA replication of JFH1 (genotype 2a) and release of the core into the culture supernatants were suppressed in DDX3 knockdown cells after inoculation of the cell culture-generated HCVcc. Thus, DDX3 is required for HCV RNA replication.
authors.affiliation: Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Okayama 700-8558, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ariumi
authors.forename: Misao
authors.initials: M
authors.lastname: Kuroki
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Abe
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Dansako
authors.forename: Masanori
authors.initials: M
authors.lastname: Ikeda
authors.forename: Takaji
authors.initials: T
authors.lastname: Wakita
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kato
mesh_terms: D045744:Cell Line, Tumor; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics / Q000502:physiology*; D006801:Humans; D012367:RNA, Viral / Q000378:metabolism*; D012093:Replicon; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; C119332:DDX3X protein, human; D053487:DEAD-box RNA Helicases