Ameliorating effect of Gardenia jasminoides extract on amyloid beta peptide-induced neuronal cell deficit.
The brains of Alzheimer's disease (AD) patients are characterized by large deposits of amyloid beta peptide (Abeta). Abeta is known to increase free radical production in nerve cells, leading to cell death that is characterized by lipid peroxidation, free radical formation, protein oxi-dation, and DNA/RNA oxidation. In this study, we selected an extract of Gardenia jasminoides by screening, and investigated its ameliorating effects on Abeta-induced oxidative stress using PC12 cells. The effects of the extract were evaluated using the 2,7 -dichlorofluorescein diacetate (DCF-DA) assay and the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. To find the active component, the ethanol extract was partitioned with hexane, chloroform, and ethyl acetate, respectively, and the active component was purified by silica-gel column chromatography and HPLC. The results suggested that Gardenia jasminoides extract can reduce the cytotoxicity of Abeta in PC 12 cells, possibly by reducing oxidative stress.
Choi
Soo Jung
SJ
Department of Food Technology, Korea University, Seoul 136-701, Korea.
Kim
Mi-Jeong
MJ
Heo
Ho Jin
HJ
Hong
Bumshik
B
Cho
Hong Yon
HY
Kim
Young Jun
YJ
Kim
Hye Kyung
HK
Lim
Seung-Taik
ST
Jun
Woo Jin
WJ
Kim
Eun-Ki
EK
Shin
Dong-Hoon
DH
D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001362:Avoidance Learning / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D032062:Gardenia / Q000737:chemistry*; D008297:Male; D008568:Memory / Q000187:drug effects; D051379:Mice; D009474:Neurons / Q000187:drug effects; D016716:PC12 Cells; D019308:Palmitic Acid / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D013037:Spatial Behavior / Q000187:drug effects
D016229:Amyloid beta-Peptides; D000975:Antioxidants; D010936:Plant Extracts; D019308:Palmitic Acid