The influence of melatonin and N-acetylcysteine in delta-aminolevulinic acid and lead induced genotoxicity in lymphocytes in vitro.
As is well known from earlier studies, the genotoxic effect of lead exposure was partly attributed to the formation of the highly reactive oxygen metabolites (ROMs) in the blood. However, lead ions have no ability to generate ROMs. Therefore, the recently published studies paid more attention to the role of delta-aminolevulinic acid (ALA) accumulation in lead-induced DNA damage. If the above-mentioned assumptions were taken into consideration, it seemed a reasonable approach to study the possible protective effects of antioxidants against genotoxic effects of lead. According to our results, N-acetylcysteine (NAC) and melatonin (MEL) were able to reduce significantly (p<0.05) the lead- and ALA-induced sister chromatid exchange frequencies in human lymphocytes in vitro. In spite of a relative reduction in the lead- and ALA-induced micronucleus formation in human lymphocytes, the reduction was not statistically significant (p>0.05). These results could be evaluated as supportive evidence for the hypothesis that increased antioxidant capacity of cells might fortify the efficiency of protective pathways against cytogenetic damage in lead exposure.
Ustundag
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Department of Pharmaceutical Toxicology, Faculty of Pharmacy, University of Ankara, 06100 Tandoğan, Ankara, Turkey.
Duydu
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Y
D000111:Acetylcysteine / Q000737:chemistry* / Q000494:pharmacology; D000622:Aminolevulinic Acid / Q000037:antagonists & inhibitors / Q000633:toxicity*; D016923:Cell Death / Q000187:drug effects / Q000235:genetics; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D007854:Lead / Q000037:antagonists & inhibitors / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D008550:Melatonin / Q000737:chemistry* / Q000502:physiology; D008875:Middle Aged; D012854:Sister Chromatid Exchange / Q000187:drug effects / Q000502:physiology
D016166:Free Radical Scavengers; D007854:Lead; D000622:Aminolevulinic Acid; D008550:Melatonin; D000111:Acetylcysteine