Isolation of avian paramyxovirus 1 from a patient with a lethal case of pneumonia.
An unknown virus was isolated from a lung biopsy sample and multiple other samples from a patient who developed a lethal case of pneumonia following a peripheral blood stem cell transplant. A random PCR-based molecular screening method was used to identify the infectious agent as avian paramyxovirus 1 (APMV-1; a group encompassing Newcastle disease virus), which is a highly contagious poultry pathogen that has only rarely been found in human infections. Immunohistochemical analysis confirmed the presence of APMV-1 antigen in sloughed alveolar cells in lung tissue from autopsy. Sequence from the human isolate showed that it was most closely related to virulent pigeon strains of APMV-1. This is the most completely documented case of a systemic human infection caused by APMV-1 and is the first report of an association between this virus and a fatal disease in a human.
Goebel
Scott J
SJ
Divisions of Infectious Disease and Molecular Medicine, David Axelrod Institute, Wadsworth Center, NYSDOH, Albany, NY 12201-2002, USA.
Taylor
Jill
J
Barr
Bradd C
BC
Kiehn
Timothy E
TE
Castro-Malaspina
Hugo R
HR
Hedvat
Cyrus V
CV
Rush-Wilson
Kim A
KA
Kelly
Cassandra D
CD
Davis
Stephen W
SW
Samsonoff
William A
WA
Hurst
Kelley R
KR
Behr
Melissa J
MJ
Masters
Paul S
PS
D000328:Adult; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001717:Birds; D017809:Fatal Outcome; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000175:diagnosis* / Q000473:pathology / Q000821:virology*; D009522:Newcastle disease virus / Q000235:genetics / Q000302:isolation & purification*; D011024:Pneumonia, Viral / Q000175:diagnosis* / Q000473:pathology / Q000821:virology*; D033581:Stem Cell Transplantation / Q000009:adverse effects
D000956:Antigens, Viral