Co-localization and interaction of DPYSL3 and GAP43 in primary cortical neurons.
Dihydropyrimidinase-like 3 (DPYSL3) and GAP43 are both involved in neurite outgrowth, a crucial process for the differentiation of neurons. The present study shows for the first time that DPYSL3 co-localizes with GAP43 in primary cortical neurons. Further co-immunoprecipitation and overlay assay showed the ability of both recombinant and endogenous DPYSL3 to bind GAP43, indicating a specific interaction between DPYSL3 and GAP43 in primary cortical neurons.
Kowara
Renata
R
National Research Council, Institute for Biological Sciences, M-54, 1200 Montreal Road, Ottawa, Ont., Canada K1A 0R6. Renata.Kowara@nrc-cnrc.gc.ca
Ménard
Michel
M
Brown
Leslie
L
Chakravarthy
Balu
B
D000818:Animals; D002478:Cells, Cultured; D019922:GAP-43 Protein / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D025941:Protein Interaction Mapping; D051381:Rats; D017207:Rats, Sprague-Dawley; D013347:Subcellular Fractions / Q000378:metabolism*
C449760:Dpysl3 protein, rat; D019922:GAP-43 Protein; D009419:Nerve Tissue Proteins