Stabilization and encapsulation of photosensitive resveratrol within yeast cell.
The photosensitive resveratrol was successfully encapsulated in yeast cells for the first time, as characterized by FT-IR spectra, fluorescence and confocal micrographs of the yeast cells, resveratrol and microcapsules. The release characteristic of the obtained yeast-encapsulated resveratrol in simulated gastric fluid was evaluated, and its storage stability as a powder was investigated at 25 degrees C/75% relative humidity (RH), 25 degrees C/90% RH and 60 degrees C under the laboratory fluorescent lighting conditions (ca. 300 lx) or in the dark. Also, the scavenging capacity of yeast-encapsulated resveratrol on DPPH radical was compared with that of non-encapsulated resveratrol. It could be demonstrated clearly that no chemical changes occurred during the encapsulation. Besides, the DPPH radical-scavenging activity increased after the encapsulation. In addition, the yeast-encapsulated resveratrol exhibited good stability, and its bioavailability was enhanced as a result of increased solubility of resveratrol and sustained releasing.
Shi
Guorong
G
College of Biological and Technology, Hunan Agricultural University, Changsha 410128, PR China. shiguorong@56.com
Rao
Liqun
L
Yu
Huazhong
H
Xiang
Hua
H
Yang
Hua
H
Ji
Runa
R
D001682:Biological Availability; D001713:Biphenyl Compounds; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D003692:Delayed-Action Preparations; D004339:Drug Compounding; D004355:Drug Stability; D004356:Drug Storage; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000737:chemistry*; D006358:Hot Temperature; D008027:Light; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010316:Particle Size; D010851:Picrates / Q000737:chemistry; D011208:Powders; D000077185:Resveratrol; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013267:Stilbenes / Q000008:administration & dosage / Q000737:chemistry*
D001713:Biphenyl Compounds; D003692:Delayed-Action Preparations; D016166:Free Radical Scavengers; D010851:Picrates; D011208:Powders; D013267:Stilbenes; C004931:1,1-diphenyl-2-picrylhydrazyl; D000077185:Resveratrol