Origin and fate of pendrin-positive intercalated cells in developing mouse kidney.
Pendrin is an apical anion exchanger found in type B and nonA-nonB intercalated cells that is involved in bicarbonate secretion. The purpose of this study was to establish the origin and fate of pendrin-positive intercalated cells in the mouse kidney. Using immunohistochemistry, we found that pendrin-positive cells first appeared in the connecting tubule at embryonic day 14 (E14) and subsequently in the medullary collecting duct at E18. Most of the pendrin-positive cells in the connecting tubule were nonA-nonB intercalated cells, wheras those in the medullary collecting duct were type B intercalated cells. In the cortical collecting duct, pendrin-positive cells appeared in the inner part at day 4 after birth and in the outer part at day 7. Pendrin-positive cells gradually disappeared by apoptosis from the inner part of the medullary collecting duct two weeks after birth. Using 5-bromo-2'deoxy-uridine (BrdU) to follow cell proliferation, we determined that selective proliferation of pendrin-positive intercalated cells does not occur; instead, these cells may arise from undifferentiated precursor cells from separate foci, one in the connecting tubule and one in the collecting duct.
Song
Hyun-Kuk
HK
Department of Anatomy and Death Disease Research Center, The Catholic University of Korea, Seoul, Korea.
Kim
Wan-Young
WY
Lee
Hyun-Wook
HW
Park
Eun-Young
EY
Han
Ki-Hwan
KH
Nielsen
Soren
S
Madsen
Kirsten M
KM
Kim
Jin
J
D000818:Animals; D000831:Animals, Newborn; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism; D027321:Anion Transport Proteins / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D047109:Fetal Development / Q000502:physiology*; D005333:Fetus / Q000378:metabolism*; D007668:Kidney / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D051379:Mice; D006180:Proton-Translocating ATPases / Q000378:metabolism; D000076988:Sulfate Transporters
D001457:Anion Exchange Protein 1, Erythrocyte; D027321:Anion Transport Proteins; C494849:Slc26a4 protein, mouse; D000076988:Sulfate Transporters; D006180:Proton-Translocating ATPases