An effective method for the in situ synthesis of DNA-CPG conjugates using chemical ligation technology as tools for SNP analysis.
In this paper, we report a new method for the SNP analysis by using a chemical ligation (CL) technique on CPG plates with high coupling efficiency. This method showed markedly high match/mismatch discrimination ability. Particularly, replacement of thymidine with 2-thiothymidine in DNA probes used in the CL technology resulted in significant improvement of the base discrimination ability of the thymine base in this system.
Ohkubo
Akihiro
A
4259, Nagatsuta, Department of Life Science, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
Tanaka
Kunihiko
K
Taguchi
Haruhiko
H
Seio
Kohji
K
Nagasawa
Hiroshi
H
Tsukahara
Toshifumi
T
Sekine
Mitsuo
M
D002851:Chromatography, High Pressure Liquid; D018899:CpG Islands; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D020641:Polymorphism, Single Nucleotide
D015342:DNA Probes; D004247:DNA