A reversed-phase high-performance liquid chromatographic method for the determination of aloesin, aloeresin A and anthraquinone in Aloe ferox.
A reversed-phase HPLC method for the quantification of aloesin, aloeresin a and anthraquinone (as barbaloin) in Aloe ferox Miller and aloe-related products has been developed and validated. The method utilized a C18 column with a water-methanol gradient and UV detection at 297 nm. The method validation included linearity, accuracy, precision, limit of detection, limit of quantitation, specificity and standard solution stability. The method showed good linearity (r > 0.99 for all components) and recovery (>85% for all components). The detection and quantitation limits for barbaloin were determined to be 0.02 and 0.1 ppm at signal-to-noise ratios of approximately 3:1 and 10:1, respectively.
Zahn
Michael
M
Unigen, 2660 Willamette Drive NE, Lacey, WA 98516, USA.
Trinh
Thao
T
Jeong
Mijeong Lee
ML
Wang
Desheng
D
Abeysinghe
Padmapriya
P
Jia
Qi
Q
Ma
Wenwen
W
D000504:Aloe / Q000737:chemistry*; D000880:Anthraquinones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002867:Chromones / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D015394:Molecular Structure; D012680:Sensitivity and Specificity
D000880:Anthraquinones; D002867:Chromones; D005960:Glucosides; C521199:aloeresin A; C069868:aloesin