Hypertonic saline solution stimulated CLC-3 production in primary cultured human nasal epithelial cells.
OBJECTIVE
To determine the molecular mechanisms of hypertonic saline on nasal disorders.


METHODS
Primary human nasal epithelial cells (HNECs) were treated with 0.9 and 3% saline for 12h. CLC-3 was detected in HNECs by immunohistochemistry, reverse transcriptase-polymerase chain reaction (RT-PCR) and western blotting.


RESULTS
Positive expression of CLC-3 was detected in HNECs in all subjects by immunohistochemistry. The relative density of CLC-3 mRNA was 0.22+/-0.09 and 0.12+/-0.05 in HNECs treated with 3 and 0.9% saline, respectively. The effect of 3% saline on the expression of CLC-3 in HNECs was observed in a time-dependent manner.


CONCLUSIONS
Hypertonic saline decreases the nasal edema and influences the mucocilliary function through the fluid secretion in vitro and may be associated with the upregulation of CLC-3.
Li
Hua-Bin
HB
Department of Otolaryngology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
Shi
Jian-Bo
JB
Cao
Fang
F
Cheng
Lei
L
Wen
Wei-Ping
WP
Xu
Geng
G
D000328:Adult; D015153:Blotting, Western; D002478:Cells, Cultured; D018118:Chloride Channels / Q000187:drug effects* / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D005260:Female; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000187:drug effects* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D015854:Up-Regulation / Q000235:genetics
D018118:Chloride Channels; C115376:ClC-3 channel; D012333:RNA, Messenger; D012462:Saline Solution, Hypertonic