Screening of genes related to methylglyoxal susceptibility.
Methylglyoxal (MG) is a reactive metabolite known to accumulate in certain physiological conditions. We attempted to isolate genes associated with this metabolite by genome-wide mutagenesis with TnphoA derivative. After screening on methylglyoxal-containing plate, we obtained insertions in three different genes, ydbD, yjjQ, and yqiI, which gave rise to reproducible MG-sensitive phenotypes in glyoxalase-deficient strain. In addition to its MG sensitivity, the insertion in yqiI exhibited an impaired motility resulting from a reduced flagellar expression.
Kim
Insook
I
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
Kim
Joonho
J
Min
Bumchan
B
Lee
Changhan
C
Park
Chankyu
C
D000096:Acetone / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D005407:Flagella / Q000235:genetics / Q000502:physiology; D005798:Genes, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D008957:Models, Genetic; D016254:Mutagenesis, Insertional / Q000187:drug effects; D009154:Mutation / Q000187:drug effects*; D011765:Pyruvaldehyde / Q000494:pharmacology*
D000096:Acetone; D019344:Lactic Acid; D011765:Pyruvaldehyde; C004433:acetol