Novel aflatoxin derivatives and protein conjugates.
Aflatoxins, a group of structurally related mycotoxins, are well known for their toxic and carcinogenic effects in humans and animals. Aflatoxin derivatives and protein conjugates are needed for diverse analytical applications. This work describes a reliable and fast synthesis of novel aflatoxin derivatives, purification by preparative HPLC and characterisation by ESI-MS and one- and two-dimensional NMR. Novel aflatoxin bovine serum albumin conjugates were prepared and characterised by UV absorption and MALDI-MS. These aflatoxin protein conjugates are potentially interesting as immunogens for the generation of aflatoxin selective antibodies with novel specificities.
Cervino
Christian
C
Institute of Hydrochemistry, Technische Universität München, Marchioninistrasse 17, 81377 München, Germany. christian.cervino@ch.tum.de
Knopp
Dietmar
D
Weller
Michael G
MG
Niessner
Reinhard
R
D000348:Aflatoxins / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013388:Succinimides / Q000737:chemistry
D000348:Aflatoxins; D013388:Succinimides; D012710:Serum Albumin, Bovine; C001426:N-hydroxysuccinimide