Simple and rapid colorimetric enzyme sensing assays using non-crosslinking gold nanoparticle aggregation.
Non-crosslinking gold nanoparticle (AuNP) aggregation induced by the loss (or screen) of surface charges is applied for enzymatic activity sensing and potentially inhibitor screening.
Zhao
Weian
W
Department of Chemistry, McMaster University, 1280 Main St. W. Hamilton, Ontario, Canada.
Chiuman
William
W
Lam
Jeffrey C F
JC
Brook
Michael A
MA
Li
Yingfu
Y
D000241:Adenosine / Q000737:chemistry / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D003124:Colorimetry / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*
D003432:Cross-Linking Reagents; D006046:Gold; D000255:Adenosine Triphosphate; D000469:Alkaline Phosphatase; D000241:Adenosine