Contribution of anaphase B to chromosome separation in higher plant cells estimated by image processing.
Anaphase can be categorized into the two subphases of anaphase A and B, but anaphase B has not been clearly described in higher plant cells. In this study, we time-sequentially followed the dynamics of chromosome segregation and spindle elongation in tobacco BY-2 cells using histone-red fluorescent protein (RFP) and green fluorescent protein (GFP)-tubulin, respectively. Construction of kymographs and determination of the positions of chromosomes and spindle edges by image processing revealed that anaphase B contributed to about 40% of the chromosome separation in distance, which is comparable with that in animal cells. These results suggest that higher plant cells potentially possess the process of anaphase B.
Hayashi
Tomomi
T
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha 5-1-5, Kashiwa, Chiba, 277-8562 Japan.
Sano
Toshio
T
Kutsuna
Natsumaro
N
Kumagai-Sano
Fumi
F
Hasezawa
Seiichiro
S
D000705:Anaphase / Q000502:physiology*; D002460:Cell Line; D032461:Chromosomes, Plant / Q000502:physiology*; D004261:DNA Replication / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D014026:Nicotiana / Q000166:cytology*