title: Dermal absorption of arsenic from soils as measured in the rhesus monkey.
abstract: Regulatory agencies have relied on dermal absorption data for soluble forms of arsenic as the technical basis for specific absorption values that are used to calculate exposure to arsenic in weathered soil. These evaluations indicate that percutaneous absorption of arsenic from soil ranges from 3.2 to 4.5% of the dermally applied dose, based on studies of arsenic freshly mixed with soil. When this value is incorporated into risk assessments and combined with other assumptions about dermal exposures to soil, the conclusion is often that dermal exposure to arsenic from soil may contribute significantly to overall exposure to arsenic in soil. Prior characterization research has indicated that the solubility of arsenic in soil varies, depending on the provenance of the soil, the source of the arsenic, and the chemical interaction of arsenic with other minerals present within the soil matrix. Weathering produces forms of arsenic that are more tightly bound within the soil and less available for absorption. Our research expands on prior in vivo studies to provide insights into the potential for dermal absorption of arsenic from the more environmentally relevant substrate of soil. Specifically, two soils with very high concentrations of arsenic were evaluated under two levels of skin hydration. One soil, containing 1400 mg/kg arsenic, was collected adjacent to a pesticide production facility in New York. The other soil, containing 1230 mg/kg arsenic, was collected from a residential area with a history of application of arsenical pesticides. Although the results of this research are constrained by the small study size dictated by the selection of an animal research model using monkeys, the statistical power was optimized by using a "crossover" study design, wherein each animal could serve as its own comparison control. No other models (animal or in vitro) were deemed adequate for studying the dermal absorption of soil arsenic. Our results show dermal absorption of soluble arsenic in solution to be 4.8 +/- 5.5%, which is similar to results reported earlier for arsenic in solution (and used by regulatory agencies in recommendations regarding dermal absorption of arsenic). Conversely, absorption following application of arsenic in the soil matrices resulted in mean estimated arsenic absorption of 0.5% or less for all soils, and all individual estimates were less than 1%. More specifically, following application of arsenic-bearing soils to the abdomens of monkeys, urinary arsenic excretion could not be readily distinguished from background. This was true across all five soil-dosing trials, including application of the two dry soils and three trials with wet soil. These findings are consistent with our understanding of the environmental chemistry of arsenic, wherein arsenic can be present in soils in complexed mineral forms. This research addresses an important component involved in estimating the true contribution of percutaneous exposures to arsenic in soil relative to exposures via ingestion. Our findings suggest that dermal absorption of arsenic from soil is truly negligible, and that EPA's current default assumption of 3% dermal absorption of arsenic from soils results in significant overestimates of exposure.
authors.affiliation: Exponent, Boulder, CO, USA. lowneyy@exponent.com
authors.forename: Yvette W
authors.initials: YW
authors.lastname: Lowney
authors.forename: Ronald C
authors.initials: RC
authors.lastname: Wester
authors.forename: Rosalind A
authors.initials: RA
authors.lastname: Schoof
authors.forename: Colleen A
authors.initials: CA
authors.lastname: Cushing
authors.forename: Melanie
authors.initials: M
authors.lastname: Edwards
authors.forename: Michael V
authors.initials: MV
authors.lastname: Ruby
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D001151:Arsenic / Q000493:pharmacokinetics* / Q000652:urine; D001682:Biological Availability; D005260:Female; D008253:Macaca mulatta / Q000378:metabolism*; D008670:Metals / Q000032:analysis; D018570:Risk Assessment; D012869:Skin Absorption; D012987:Soil / Q000032:analysis; D012989:Soil Pollutants / Q000493:pharmacokinetics* / Q000652:urine
chemical_list: D008670:Metals; D012987:Soil; D012989:Soil Pollutants; D001151:Arsenic