title: Antibacterial functionalization of an experimental self-etching primer by inorganic agents: microbiological and biocompatibility evaluations.
abstract: Antibacterial activities have been demonstrated on oral bacteria with inorganic antibacterial agents (ABAs) after their incorporations into an experimental self-etching primer (ESP) before curing. This study was to assess their biocompatibility and antibacterial activity after curing. Six ABAs were incorporated respectively into ESP for treating specimens. After curing, their bactericidal activities on Streptococcus mutans and influences to the early bacterial colonization were assessed by direct contact and viable count. Systemic toxicity in rats after short-term oral exposure and direct contact cytotoxicity with NIH3T3 fibroblasts were tested. Incorporation of ZnOw AT-83, Longbei antibiotic, Antim-AMS2 or IONPURE-H significantly enhanced the antibacterial effect of ESP after curing, even after 1 month aging. Specimens treated by ESP with ZnOw AT-83, Longbei antibiotic or Antim-AMS2 showed slightly less bacterial adhesion than control. Animal experiments revealed neither toxic signs nor significant differences in body weight gain between control and other groups. Cell vitality or proliferation rates were ranged from 76% to 100% with respect to controls. Basic magnesium hypochlorite, ZnOw AT-83 and ZnOw AT-88 were less toxic. Toxicity only observed in areas beneath the specimens and/or in the direct vicinity of the specimen edge. From microbiological and biocompatibility aspects, the tested ABAs can be effectively incorporated in ESP to provide antibacterial activity against S. mutans. ZnOw AT-83 was the most promising one.
authors.affiliation: Department of Prosthodontics, College of Stomatology, Fourth Military Medical University, 710032 Xi'an, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Fang
authors.forename: Feng
authors.initials: F
authors.lastname: Chai
authors.forename: Ji-Hua
authors.initials: JH
authors.lastname: Chen
authors.forename: Christel
authors.initials: C
authors.lastname: Neut
authors.forename: Min
authors.initials: M
authors.lastname: Jia
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: San-Jun
authors.initials: SJ
authors.lastname: Zhao
authors.forename: Hartmut F
authors.initials: HF
authors.lastname: Hildebrand
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D001422:Bacterial Adhesion / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D008297:Male; D008422:Materials Testing; D051379:Mice; D008826:Microbial Sensitivity Tests; D041681:NIH 3T3 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D019279:Resin Cements / Q000494:pharmacology* / Q000633:toxicity; D013295:Streptococcus mutans / Q000187:drug effects*; D013499:Surface Properties; D023382:Toxicity Tests, Acute
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D019279:Resin Cements; C429625:Transbond Plus Self-Etching Primer