MegaPlex PCR: a strategy for multiplex amplification.
'MegaPlex PCR' is a robust technology for highly multiplexed amplification of specific DNA sequences. It uses target-specific pairs of PCR primers that are physically separated by surface immobilization. Initial surface-based amplification cycles are then coupled to efficient solution-phase PCR using one common primer pair. We demonstrate this method by co-amplifying and genotyping 75 unselected human single-nucleotide polymorphism (SNP) loci.
Meuzelaar
Linda Strömqvist
LS
Department of Genetics, University of Leicester, University Road, Leicester, LE1 7RH, UK.
Lancaster
Owen
O
Pasche
J Paul
JP
Kopal
Guido
G
Brookes
Anthony J
AJ
D017931:DNA Primers; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers