title: Development of genetic instability in mice in response to chronic irradiation simulating high-altitude flight conditions.
abstract: The purpose of this work was to study the chronic influence of the high-energy radiation field formed in the atmosphere at an altitude of 10 to 30 km on the level of DNA damage in leukocytes of peripheral blood in mice. The external radiation field (behind the concrete shield) of the U-70 accelerator (Serpukhov, Russia) was used for these studies. This radiation field simulates the components and spectral composition of the high-energy radiation field formed in the atmosphere at an altitude of 10 to 30 km. Two groups of SHK line mice were chronically irradiated with a total dose equivalent to 21.5 and 31.5 cGy. The state of the genome of nucleated blood cells was assessed by the Comet assay (alkaline version) 72 h after completion of chronic irradiation. The level of genome damage in individual peripheral blood leukocytes of irradiated animals was compared with the basal level of DNA lesions in peripheral blood leukocytes of unirradiated control mice. The damage was expressed in %TDNA (the amount of DNA found in the "comet tail" in percent of total DNA in the "comet"). It was found that in mice exposed to the radiation field of the accelerator, the mean value of DNA damage was: %TDNA = 3.88 +/- 0.35% for a dose of 21.5 cGy and % TDNA = 6.00 +/- 0.82% for a dose of 31.5 cGy. In mice irradiated at an X-ray therapeutic device with a dose of 150 cGy 24 h before the examination, %TDNA was 2.27 +/- 0.34% and this did not differ from %TDNA in unirradiated mice, 2.68 +/- 0.56%. We suggest that the increased level of DNA damage observed in mice irradiated with 31.5 cGy from the mixed radiation field at the Serpukhov accelerator points to the development of genetic instability in their leukocytes as a result of chronic exposure of animals to this particular radiation field.
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino. sirota@iteb.ru
authors.forename: N P
authors.initials: NP
authors.lastname: Sirota
authors.forename: E A
authors.initials: EA
authors.lastname: Kuznetsova
authors.forename: V N
authors.initials: VN
authors.lastname: Peleshko
mesh_terms: D000531:Altitude; D000818:Animals; D020552:Comet Assay; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D042822:Genomic Instability / Q000528:radiation effects*; D007962:Leukocytes / Q000528:radiation effects / Q000648:ultrastructure; D051379:Mice; D008815:Mice, Inbred Strains; D011829:Radiation Dosage; D013026:Space Flight; D018561:Space Simulation