title: Oligopotent mesenchymal stem cell-like clone becomes multinucleated following phorbol ester, TPA stimulation.
abstract: We established a mesenchymal stem cell clone, 5F9A, from rat bone marrow substrate adherent cells by repeated limiting dilutions. The cells have a fibroblastic shape and form intimate contacts with adjacent cells with interdigitations and junctions similar to adherence and tight junctions in a semi-confluent culture. Analysis of the phenotypes of these cells by RT-PCR and FACS demonstrated that they resembled mesenchymal stem cells, and the cells could differentiate into adiopocytes and osteoblasts under appropriate conditions in vitro showing their oligopotency. Furthermore, the cells were induced to become multinuclear cells by TPA (12-o-tetradecanoylphorbol 13-acetate) stimulation.
authors.affiliation: Department of Histology and Cell Biology, Yokohama City University School of Medicine, Yokohama, Japan. k6552@yokohama-cu.ac.jp
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Yoshida
authors.forename: Michio
authors.initials: M
authors.lastname: Ono
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Maejima
authors.forename: Michiyo
authors.initials: M
authors.lastname: Esaki
authors.forename: Hajime
authors.initials: H
authors.lastname: Sawada
mesh_terms: D017667:Adipocytes / Q000648:ultrastructure*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure*; D007365:Intercellular Junctions / Q000648:ultrastructure; D059630:Mesenchymal Stem Cells / Q000502:physiology* / Q000648:ultrastructure; D010006:Osteoblasts / Q000648:ultrastructure*; D010641:Phenotype; D010703:Phorbol Esters; D051381:Rats
chemical_list: D010703:Phorbol Esters