title: Babesia gibsoni: identification of an immunodominant, interspersed repeat antigen.
abstract: In this report, an immunodominant antigen called BgIRA from Babesia gibsoni is identified and described. A highly repetitive antigen was screened from a cDNA library. The genomic BgIRA gene exists as single cope gene and contains 10 introns. BgIRA plays a dominant role in the immune response in dogs infected with B. gibsoni. The specificity and sensitivity of the rBgIRA in an ELISA indicated that this antigen might be useful in a diagnostic test.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Honglin
authors.initials: H
authors.lastname: Jia
authors.forename: M Alaa
authors.initials: MA
authors.lastname: Terkawi
authors.forename: Gabriel Oluga
authors.initials: GO
authors.lastname: Aboge
authors.forename: Youn-Kyoung
authors.initials: YK
authors.lastname: Goo
authors.forename: Jinlin
authors.initials: J
authors.lastname: Zhou
authors.forename: Eung-Goo
authors.initials: EG
authors.lastname: Lee
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Ikuo
authors.initials: I
authors.lastname: Igarashi
authors.forename: Kozo
authors.initials: K
authors.lastname: Fujisaki
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D001403:Babesia / Q000235:genetics / Q000276:immunology*; D001404:Babesiosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D004283:Dog Diseases / Q000175:diagnosis / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015723:Gene Library; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D012680:Sensitivity and Specificity; D013047:Specific Pathogen-Free Organisms
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016056:Immunodominant Epitopes