title: Two new anamorphic yeasts, Candida thailandica sp. nov. and Candida lignicola sp. nov., isolated from insect frass in Thailand.
abstract: Two new yeast strains of the genus Candida were isolated from insect frass collected in Khao-Yai National Park, Nakhonrachasima, Thailand. Based on the morphological, physiological and chemotaxonomic characteristics, and sequence analysis of the D1/D2 domain of 26S rRNA gene, these two strains were found to represent two distinct undescribed species and were named Candida thailandica sp. nov. (ST-17 = BCC 7717(T) = NBRC 102562(T)=CBS 10 610) and Candida lignicola sp. nov. (ST-33 = BCC 7733(T) = NBRC 102564(T) = CBS 10612). In the D1/D2 domain of 26S rRNA gene, C. thailandica (GeneBank accession no. AY228491) differs from Candida tsuchiyae, the nearest species, in 66 nucleotide substitutions (10%) and C. lignicola (GeneBank accession no. AY845350) differs from Candida coipomoensis, the nearest species, in nine nucleotides (1.6%). These two new species are clearly distinguished from their closest species by the assimilation of several carbon compounds.
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand. sasitorn@biotec.or.th
authors.forename: Sasitorn
authors.initials: S
authors.lastname: Jindamorakot
authors.forename: Savitree
authors.initials: S
authors.lastname: Limtong
authors.forename: Wichien
authors.initials: W
authors.lastname: Yongmanitchai
authors.forename: Manee
authors.initials: M
authors.lastname: Tuntirungkij
authors.forename: Wanchern
authors.initials: W
authors.lastname: Potacharoen
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakase
mesh_terms: D000818:Animals; D001483:Base Sequence; D002175:Candida / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000382:microbiology; D007313:Insecta / Q000382:microbiology*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013785:Thailand
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S