title: [Study on the kinesis of cellular immunity in adults vaccinated with recombinant hepatitis B vaccine].
abstract: OBJECTIVE
  To evaluate the kinesis of cellular immunity in adults who were vaccinated with yeast recombinant hepatitis B(rHB) vaccine and the correlation between cellular and humoral immune responses induced by the vaccine.
  
  
  METHODS
  Eight adults were vaccinated with rHB vaccine according to 0, 1,2 month schedule. The peripheral blood mononuclear cells(PBMCs) were collected at the 3, 8, 21, 34 and 65 days after the first dose. The high purity of CD4+ and CD8+ T cells obtained by sorting from PBMCs were restimulated with recombinant hepatitis B surface antigens (rHBsAg) or peptides. The spot forming cell (SFC) of IFN-gamma, IL-2 and IL-4 of CD4+ and CD8+ T cells were detected by enzyme-linked immunospot (ELISPOT).
  
  
  RESULTS
  The characteristics of IFN-gamma, IL-2 and IL-4 of CD4+ and CD8+ T cells appeared different after immunization with rHB vaccine. IFN-gamma of CD8+ and CD4+ T cells could be detected early with stable SFC, while the IL-2 and IL-4 of CD4+ T cells appeared late but increased after the second and third dose of vaccination. The positive rate of IL-4 of CD4+ T cells were significantly correlated with the positive rate of anti-HBs, while the SFCs of IL-4 and IL-2 of CD4+ T cells were also significantly related to the titers of anti-FIBs.
  
  
  CONCLUSIONS
  IFN-gamma could be detected early after rHB vaccination in adults, and the positive rates of IL-4 and IL-2 were correlated with that of anti-HBs.
authors.affiliation: National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
authors.forename: Zhong-yu
authors.initials: ZY
authors.lastname: Hu
authors.forename: Feng-cai
authors.initials: FC
authors.lastname: Zhu
authors.forename: Peng
authors.initials: P
authors.lastname: He
authors.forename: She-lan
authors.initials: SL
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Xing
authors.initials: X
authors.lastname: Fang
authors.forename: Xiang-jun
authors.initials: XJ
authors.lastname: Zhai
authors.forename: Shao-hui
authors.initials: SH
authors.lastname: Qiu
authors.forename: Zheng-lun
authors.initials: ZL
authors.lastname: Liang
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: He-min
authors.initials: HM
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Zhuang
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D007115:Immunization Schedule; D007371:Interferon-gamma / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D017325:Hepatitis B Vaccines; D007376:Interleukin-2; D014614:Vaccines, Synthetic; D015847:Interleukin-4; D007371:Interferon-gamma