title: Identification of major phenolic compounds of Chinese water chestnut and their antioxidant activity.
abstract: Chinese water chestnut (CWC) is one of the most popular foods among Asian people due to its special taste and medical function. Experiments were conducted to test the antioxidant activity and then determine the major phenolic compound components present in CWC. CWC phenolic extract strongly inhibited linoleic acid oxidation and exhibited a dose-dependent free-radical scavenging activity against alpha,alpha-diphenyl-beta-picrylhydrazyl (DPPH) radicals, superoxide anions and hydroxyl radicals, which was superior to ascorbic acid and butylated hydroxytoluene (BHT), two commercial used antioxidants. Furthermore, the CWC extract was found to have a relatively higher reducing power, compared with BHT. The major phenolic compounds present in CWC tissues were extracted, purified and identified by high-performance liquid chromatograph (HPLC) as (-)-gallocatechin gallate, (-)-epicatechin gallate and (+)-catechin gallate. This study suggests that CWC tissues exhibit great potential for antioxidant activity and may be useful for their nutritional and medicinal functions.
authors.affiliation: South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou Leyiju 510650, PR China. yanliyou2002@163.com
authors.forename: Yanli
authors.initials: Y
authors.lastname: You
authors.forename: Xuewu
authors.initials: X
authors.lastname: Duan
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wei
authors.forename: Xinguo
authors.initials: X
authors.lastname: Su
authors.forename: Mouming
authors.initials: M
authors.lastname: Zhao
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Neungnapa
authors.initials: N
authors.lastname: Ruenroengklin
authors.forename: Yueming
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D002084:Butylated Hydroxytoluene / Q000737:chemistry; D002392:Catechin / Q000031:analogs & derivatives / Q000737:chemistry; D002621:Chemistry / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D031256:Eleocharis / Q000378:metabolism*; D005609:Free Radicals; D006834:Hydrazines / Q000737:chemistry; D019787:Linoleic Acid / Q000737:chemistry; D015227:Lipid Peroxidation; D008956:Models, Chemical; D019800:Phenol / Q000032:analysis* / Q000737:chemistry; D010851:Picrates
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005609:Free Radicals; D006834:Hydrazines; D010851:Picrates; D002084:Butylated Hydroxytoluene; D019800:Phenol; D002392:Catechin; C062669:epicatechin gallate; D019787:Linoleic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl