Primary rat monocytes migrate through a BCEC-monolayer and express microglia-markers at the basolateral side.
Monocytes are pluripotent cells of the immune system, circulate in the blood and cross the blood-brain barrier continuously through life. The aim of this study was to explore if primary rat monocytes can adhere and transmigrate at a monolayer of brain capillary endothelial cells (BCEC) and if the monocytes undergo differentiation toward a microglial phenotype at the basolateral side. Monocytes and as a control primary microglia were immunohistochemically stained with markers for CD68 (clone ED-1), CD11b (clone OX-42) or CD11c (clone 8A2). The primary rat monocytes (100,000 cells added) adhered at the BCEC-monolayer (approx. 1200 cells/well) within 30 min and migrated to the basolateral side within 18 h (approx. 40,000 cells/well). The transmigrated monocytes partly differentiated and expressed microglia-markers at the basolateral side. Tumor necrosis factor-alpha as well as conditioned medium derived from BCEC stimulated the differentiation of monocytes in culture. In conclusion, monocytes adhere and migrate through a BCEC-monolayer and express microglia-markers at the basolateral side.
Moser
Karma V
KV
Laboratory of Psychiatry and Experimental Alzheimer's Research, Department of Psychiatry, Innsbruck Medical University, Anichstr 35, A-6020, Innsbruck, Austria.
Humpel
Christian
C
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D015703:Antigens, CD / Q000145:classification / Q000378:metabolism*; D001921:Brain / Q000166:cytology; D002452:Cell Count / Q000379:methods; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000494:pharmacology; D018920:Coculture Techniques / Q000379:methods; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000737:chemistry / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D017628:Microglia / Q000502:physiology*; D009000:Monocytes / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D015703:Antigens, CD; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D017077:Culture Media, Conditioned; D014409:Tumor Necrosis Factor-alpha