Diagnostic potential of recombinant protein of hexahistidine tag and infectious bursal disease virus VPX expressed in Escherichia coli.
The current method to detect antibody titre against infectious bursal disease virus (IBDV) in chickens is based on enzyme-linked immunosorbent assay (ELISA) using whole virus as coating antigen. Coating the ELISA plates requires a purified or at least semi-purified preparation of virus as antigen, which needs special skills and techniques. In this study, instead of using whole virus, recombinant protein of hexahistidine tag (His 6 tag) and VPX protein of IBDV expressed in E. coli was used as an alternative antigen to coat the ELISA plates. There was a good correlation coefficient (R2 = 0.972) between the results of the ELISA using plates coated with monoclonal antibody against His 6 tag and those of the commercial IBDV ELISA kit. Hence, His 6 tag and VPX recombinant protein expressed in E. coli has the potential for the development of ELISA for the measurement of IBDV-specific antibody.
Hosseini
S D
SD
Razi Research Institute, Arak, Iran.
Omar
A R
AR
Aini
I
I
Ali
A M
AM
D000818:Animals; D000956:Antigens, Viral; D018175:Birnaviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D015153:Blotting, Western / Q000662:veterinary; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D004926:Escherichia coli / Q000821:virology; D006639:Histidine / Q000235:genetics / Q000276:immunology*; D007243:Infectious bursal disease virus / Q000235:genetics / Q000276:immunology*; D009842:Oligopeptides / Q000235:genetics / Q000276:immunology*; D011201:Poultry Diseases / Q000175:diagnosis*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics / Q000276:immunology
D000956:Antigens, Viral; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D011994:Recombinant Proteins; D054334:Viral Regulatory and Accessory Proteins; D006639:Histidine