The DEAD box RNA helicase VBH-1 is required for germ cell function in C. elegans.
Vasa and Belle are conserved DEAD box RNA helicases required for germ cell function. Homologs of this group of proteins in several species, including mammals, are able to complement a mutation in yeast (DED1) suggesting that their function is highly conserved. It has been proposed that these proteins are required for mRNA translation regulation, but their specific mechanism of action is still unknown. Here we describe functions of VBH-1, a C. elegans protein closely related to Belle and Vasa. VBH-1 is expressed specifically in the C. elegans germline, where it is associated with P granules, the C. elegans germ plasm counterpart. vbh-1(RNAi) animals produce fewer offspring than wild type because of defects in oocyte and sperm production, and embryonic lethality. We also find that VBH-1 participates in the sperm/oocyte switch in the hermaphrodite gonad. We conclude that VBH-1 and its orthologs may perform conserved roles in fertility and development.
Salinas
L Silvia
LS
Departamento de Biología Celular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, México D.F., 04510 México.
Maldonado
Ernesto
E
Macías-Silva
Marina
M
Blackwell
T Keith
TK
Navarro
Rosa E
RE
D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics; D004625:Embryo, Nonmammalian; D005260:Female; D005298:Fertility / Q000235:genetics; D005854:Germ Cells / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008954:Models, Biological; D009928:Organ Specificity; D010802:Phylogeny; D017385:Sequence Homology; D013091:Spermatogenesis / Q000235:genetics; D014018:Tissue Distribution
D029742:Caenorhabditis elegans Proteins; D029721:Drosophila Proteins; C524973:VBH-1 protein, C elegans; C088269:vas protein, Drosophila; D053487:DEAD-box RNA Helicases