title: Ectropis obliqua picorna-like virus IRES-driven internal initiation of translation in cell systems derived from different origins.
abstract: Ectropis obliqua picorna-like virus (EoPV) is an insect RNA virus that causes a lethal granulosis infection of larvae of the tea looper (Ectropis obliqua). An internal ribosome entry site (IRES) mediates translation initiation of EoPV RNA. Here, bicistronic constructs were used to examine the 5' untranslated region (UTR) of EoPV for IRES activity. The capacities of the EoPV 5' UTR IRES and another insect virus IRES, the cricket paralysis virus intergenic region IRES, to mediate internal translation initiation in a variety of translation systems were also compared. The results demonstrated that the EoPV IRES functioned efficiently not only in mammalian cell-derived systems, but also in an insect cell-derived translation system. However, it functioned inefficiently in a plant cell-derived translation system. This study reveals the host preferences of the EoPV IRES and important differences in IRES function between the EoPV IRES and other characterized picorna-like insect viral IRESs.
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, China.
authors.forename: Yuanyang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zong
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, China.
authors.forename: Junping
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, China.
authors.forename: Jiamin
authors.initials: J
authors.lastname: Zhang
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D016679:Genome, Viral; D006135:Gryllidae / Q000821:virology; D007313:Insecta / Q000821:virology; D007438:Introns; D010849:Picornaviridae / Q000235:genetics*; D010944:Plants / Q000821:virology; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics; D012270:Ribosomes / Q000821:virology
chemical_list: D020121:5' Untranslated Regions; D012367:RNA, Viral