title: Nanosensor detection of an immunoregulatory tryptophan influx/kynurenine efflux cycle.
abstract: Mammalian cells rely on cellular uptake of the essential amino acid tryptophan. Tryptophan sequestration by up-regulation of the key enzyme for tryptophan degradation, indoleamine 2,3-dioxygenase (IDO), e.g., in cancer and inflammation, is thought to suppress the immune response via T cell starvation. Additionally, the excreted tryptophan catabolites (kynurenines) induce apoptosis of lymphocytes. Whereas tryptophan transport systems have been identified, the molecular nature of kynurenine export remains unknown. To measure cytosolic tryptophan steady-state levels and flux in real time, we developed genetically encoded fluorescence resonance energy transfer nanosensors (FLIPW). The transport properties detected by FLIPW in KB cells, a human oral cancer cell line, and COS-7 cells implicate LAT1, a transporter that is present in proliferative tissues like cancer, in tryptophan uptake. Importantly, we found that this transport system mediates tryptophan/kynurenine exchange. The tryptophan influx/kynurenine efflux cycle couples tryptophan starvation to elevation of kynurenine serum levels, providing a two-pronged induction of apoptosis in neighboring cells. The strict coupling protects cells that overproduce IDO from kynurenine accumulation. Consequently, this mechanism may contribute to immunosuppression involved in autoimmunity and tumor immune escape.
authors: Department of Plant Biology, Carnegie Institution, Stanford, California, United States of America.
authors: Thijs
authors: T
authors: Kaper
authors: Loren L
authors: LL
authors: Looger
authors: Hitomi
authors: H
authors: Takanaga
authors: Michael
authors: M
authors: Platten
authors: Lawrence
authors: L
authors: Steinman
authors: Wolf B
authors: WB
authors: Frommer
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D019556:COS Cells; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D003600:Cytosol / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D007108:Immune Tolerance / Q000502:physiology*; D007737:Kynurenine / Q000378:metabolism*; D027283:Large Neutral Amino Acid-Transporter 1 / Q000378:metabolism; D036103:Nanotechnology / Q000295:instrumentation*; D013601:T-Lymphocytes; D014364:Tryptophan / Q000378:metabolism*
chemical_list: D027283:Large Neutral Amino Acid-Transporter 1; D007737:Kynurenine; D014364:Tryptophan