Histological assessments on the abnormalities of mouse epiphyseal chondrocytes with short term centrifugal loading.
We have examined the morphological changes in chondrocytes after exposure to experimental hypergravity. Tibial epiphyseal cartilages of 17-days-old mouse fetuses were exposed to centrifugation at 3G for 16 h mimicking hypergravitational environment (experimental group), or subjected to stationary cultures (control group). Centrifugation did not affect the sizes of epiphyseal cartilage, chondrocyte proliferation, type X collagen-positive hypertrophic zone, and the mRNA expressions of parathyroid hormone-related peptide and fibroblast growth factor receptor III. However, centrifuged chondrocytes showed abnormal morphology and aberrant spatial arrangements, resulting in disrupted chondrocytic columns. Through histochemical assessments, actin filaments were shown to distribute evenly along cell membranes of control proliferative chondrocytes, while chondrocytes subjected to centrifugal force developed a thicker layer of actin filaments. Transmission electron microscopic observations revealed spotty electron-dense materials underlying control chondrocytes' cell membranes, while experimental chondrocytes showed their thick layer. In the intracolumnar regions of the control cartilage, longitudinal electron-dense fibrils were associated with short cytoplasmic processes of normal chondrocytes, indicating assumed cell-tomatrix interactions. These extracellular fibrils were disrupted in the centrifuged samples. Summarizing, altered actin filaments associated with cell membranes, irregular cell shape and disappearance of intracolumnar extracellular fibrils suggest that hypergravity disturbs cell-to-matrix interactions in our cartilage model.
de Freitas
Paulo Henrique Luiz
PH
Division of Oral and Maxillofacial Surgery, Niigata University Graduate School of Medical and Dental Sciences, Japan.
Kojima
Taku
T
Ubaidus
Sobhan
S
Li
Minqi
M
Shang
Guangwei
G
Takagi
Ritsuo
R
Maeda
Takeyasu
T
Oda
Kimimitsu
K
Ozawa
Hidehiro
H
Amizuka
Norio
N
D008841:Actin Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D049109:Cell Proliferation; D048430:Cell Shape; D002498:Centrifugation / Q000009:adverse effects; D019902:Chondrocytes / Q000378:metabolism* / Q000648:ultrastructure*; D024121:Collagen Type X / Q000096:biosynthesis; D005109:Extracellular Matrix / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D005333:Fetus / Q000378:metabolism* / Q000648:ultrastructure*; D006132:Growth Plate / Q000002:abnormalities / Q000378:metabolism* / Q000648:ultrastructure*; D018471:Hypergravity / Q000009:adverse effects*; D051379:Mice; D008813:Mice, Inbred ICR; D046529:Microscopy, Electron, Transmission; D044162:Parathyroid Hormone-Related Protein / Q000096:biosynthesis; D011247:Pregnancy; D051498:Receptor, Fibroblast Growth Factor, Type 3 / Q000096:biosynthesis; D013997:Time Factors; D046509:Tissue Culture Techniques
D024121:Collagen Type X; D044162:Parathyroid Hormone-Related Protein; C496366:Fgfr3 protein, mouse; D051498:Receptor, Fibroblast Growth Factor, Type 3