Total internal reflection fluorescence spectroscopy for investigating the adsorption of a porphyrin at the glass/water interface in the presence of a cationic surfactant below the critical micelle concentration.
Total internal reflection fluorescence (TIRF) spectroscopy was used to investigate the adsorption behavior of meso-tetrakis(p-sulfonatophenyl)porphyrin (TPPS) at the glass/water interface in the presence of a cationic surfactant (cetyltrimethylammonium bromide, CTAB) far below the critical micelle concentration. The adsorption model of TPPS at the glass/water interface in the presence of low concentration of CTAB was proposed, which was different from the adsorption of TPPS in the presence of micelles of CTAB at the glass/water interface. TPPS and CTAB did not form stable complex at the interface in dilute system. The interfacial species of TPPS were analyzed by comparing the spectra of TPPS at the glass/water interface and in the aqueous phase. The influences of the TPPS concentration, the CTAB concentration, and the pH values on the interfacial fluorescence spectra and intensities were studied. It was demonstrated that electrostatic interaction and hydrophobicity performed an important role on the adsorption of TPPS in the presence of CTAB. The different effects of TPPS concentration on the adsorption behaviour of TPPS at different pH were observed for the first time. It was found that the adsorption isotherms of TPPS at glass/water interface could fit Freundlich equation at pH 7.1.
Tang
Yao-Ji
YJ
Department of Chemistry and The Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.
Chen
Ying
Y
Yao
Min-Na
MN
Zou
Zhe-Xiang
ZX
Han
Guo-Bin
GB
Li
Yao-Qun
YQ
D000327:Adsorption; D005898:Glass / Q000737:chemistry*; D008823:Micelles; D015394:Molecular Structure; D011166:Porphyrins / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry*; D013816:Thermodynamics; D014867:Water / Q000737:chemistry*
D008823:Micelles; D011166:Porphyrins; D013501:Surface-Active Agents; D014867:Water