Identification of genes associated with tumorigenesis of meibomian cell carcinoma by microarray analysis.
Meibomian cell carcinoma (MCC) is a malignant tumor of the meibomian glands located in the eyelids. No information exists on the cytogenetic and genetic aspects of MCC. There is no report on the gene expression profile of MCC. Thus there is a need, for both scientific and clinical reasons, to identify genes and pathways that are involved in the development and progression of MCC. We analyzed the gene expression profile of MCC by the microarray technique. Forty-four genes were upregulated and 149 genes were downregulated in MCC. Differential expression data were confirmed for 5 genes by semiquantitative RT-PCR in MCC tumors: GTF2H4, RBM12, UBE2D3, DDX17, and LZTS1. We found dysregulation of two major pathways in MCC: MAPK and JAK/STAT. Clusters of genes on chromosomes 1, 12, and 19 were dysregulated in MCC. The data presented here will facilitate the identification of specific markers and therapeutic targets for the treatment of MCC patients.
Kumar
Arun
A
Department of Molecular Reproduction, Development, and Genetics, Indian Institute of Science, Bangalore 560012, India. karun@mrdg.iisc.ernet.in
Kumar Dorairaj
Syril
S
Prabhakaran
Venkatesh C
VC
Prakash
D Ravi
DR
Chakraborty
Sanjukta
S
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005142:Eyelid Neoplasms / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008537:Meibomian Glands / Q000473:pathology*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
D009363:Neoplasm Proteins; D012333:RNA, Messenger