title: Validity of MAST-CLA for diagnosis of arthropod allergy using receiver operating characteristic (ROC) analysis.
abstract: Many allergists are currently focusing on the development of new diagnostic tools, and are attempting to improve both the sensitivity and specificity. A multiple allergen simultaneous test-chemiluminescent assay (MASTCLA) is one of the most popular diagnostic tools used in the Republic of Korea. However, there remains controversy among allergists with regard to the cut-off point for a positive result. The present study was conducted in order to determine the validity of MAST-CLA as compared with that of the skin prick test, with particular emphasis on arthropod allergens, on the basis of percentage agreement rates and kappa-values, and also to suggest the optimal positive cutoff points using receiver operating characteristic (ROC) curves. The study was conducted with 97 subjects (54 men, 43 women). Optimal individual cut-off points were calculated as follows; class II for Dermatophagoides farinae, class I for Dermatophagoides pteronyssinus, and trace for a cockroach mix. These findings suggest that attempting to apply optimal individual cut-off points will be a good way of improving diagnostic tests, particularly MAST-CLA.
authors: Department of Pediatrics, College of Medicine, Soonchunhyang University, Cheonan 330-090, Korea.
authors: Joon-Soo
authors: JS
authors: Park
authors: Hae-Seon
authors: HS
authors: Nam
authors: Yong-Bae
authors: YB
authors: Kim
authors: Young-Jin
authors: YJ
authors: Choi
authors: Sang-Han
authors: SH
authors: Lee
authors: Sung-Ho
authors: SH
authors: Kim
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D003058:Cockroaches / Q000737:chemistry; D040001:Dermatophagoides farinae / Q000737:chemistry; D040002:Dermatophagoides pteronyssinus / Q000737:chemistry; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D019476:Insect Proteins / Q000276:immunology*; D008163:Luminescent Measurements / Q000379:methods* / Q000592:standards; D008297:Male; D012372:ROC Curve; D012882:Skin Tests / Q000379:methods
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D019476:Insect Proteins