Tissue engineering for full-thickness burns: a dermal substitute from bench to bedside.
Our aim was to obtain a viable and easily available dermal substitute (DS) for the definitive coverage of full-thickness burns. A DS composed of a collagen-glycosaminoglycan-chitosan dermal matrix (DM) colonized with foreskin fibroblasts (FF) is described. FF-colonized DS were compared to the DM seeded with adult dermal fibroblasts (DF). FF-colonized DS expressed more fibrillin and tropoelastin than that with DF. Reconstructed skin obtained with both FF- and DF-colonized DS similarly expressed laminin-5 and collagen VII at the dermal-epidermal junction. Both FF- and DF-colonized DS produced cutaneous wound healing mediators in a dose-dependent manner in the presence of platelet lysate. After freeze-thawing, the FF-colonized DS were recovered in culture and retained their ability to produce vascular endothelial growth factor. Grafting of DS into nude rats achieved a complete healing of a dermal-epidermal lesion with a good epidermalization.
Kellouche
Sabrina
S
INSERM, U553 and Université Paris 7, IUH, Paris 75010, France.
Martin
Christophe
C
Korb
Gregory
G
Rezzonico
Roger
R
Bouard
Delphine
D
Benbunan
Marc
M
Dubertret
Louis
L
Soler
Christophe
C
Legrand
Chantal
C
Dosquet
Christine
C
D000328:Adult; D000818:Animals; D002056:Burns / Q000503:physiopathology / Q000601:surgery / Q000628:therapy*; D002470:Cell Survival; D018925:Chemokines / Q000378:metabolism; D020405:Dermis / Q000254:growth & development / Q000378:metabolism / Q000601:surgery; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005615:Freezing; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D008856:Microscopy, Fluorescence; D051381:Rats; D011923:Rats, Nude; D012867:Skin / Q000166:cytology / Q000378:metabolism; D016038:Skin Transplantation / Q000379:methods; D017768:Skin, Artificial; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D014183:Transplantation, Heterologous; D014945:Wound Healing
D018925:Chemokines; D016326:Extracellular Matrix Proteins; D036341:Intercellular Signaling Peptides and Proteins