[SRAP fragments linked to spines of Carthamus tinctorius].
Sequence related amplified polymorphism (SRAP) technique was used to identify SRAP fragment linked to Carthamus tinctorius L. spines of outer involucral bract (OIB) , experimental evidence for molecular marker assistant breeding of Carthamus tinctorius L. has been provided. Based on the strategy of bulk segregate analysis (BSA), two gene pools were separately constructed according to the extreme trait of OIB with many long spines and no spines from Carthamus tinctorius L. Forty-five pairs of SRAP primers were selected and screened from two parents and two gene pools, and one SRAP marker M3E3 was found to be linked to the spines in segregating F2 population confirmation. M3E3 SRAP band was excised, cloned and sequenced. In 20 spininess individuals, this marker was present in 16 spininess individuals and absent in 4 individuals. This band was absent in the 15 spineless F, segregating individuals, which accounted for 11.4% recombination. The M3E3 extract length was 349bp, of which the base components of A + T accounted for 41. 08%. One SRAP marker M3E3 linked to the spines in Carthamus tinctorius L. will be of good use for breeding spineless cultivars at the molecular level in the future.
Guo
Qing-hua
QH
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Guo
Mei-li
ML
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods; D001483:Base Sequence; D031187:Carthamus tinctorius / Q000033:anatomy & histology / Q000235:genetics*; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers