[Fabrication of sensor for reactive oxygen species using gold electrodes modified with electropolymerized porphyrins and application for detection of stress of plants].
Reactive Oxygen Spiecies (ROS) such as superoxide anion radical (.O(2)(-)) act as signals for the activation of stress-response and defense pathways. However, excess ROS generated by perturbing .O(2)(-) homeostasis stimulated many environmental stress, including intense light, drought, temperature stress, herbicides, induce high radical toxicity. Consequently, quantitative analysis of .O(2)(-) is a subject of intense research, since most of ROS are derived from .O(2)(-). Iron meso-tetrakis(3-thienyl)porphyrin complexes were electropolymerized onto a Au wire electrode. The modified Au electrode were applied to .O(2)(-) sensor to detect catalytic oxidation current of .O(2)(-) which was generated as an intermediate during the oxidation of xanthine by catalystic XOD. It was revealed that the sensor was quantitative to measure .O(2)(-). The modified Au electrode were applied to measure oxidation current of .O(2)(-) in mung beans under environmental stress condition. Plants were grown in atmosphere, 25 degrees C and in black darkness. The other plants were exposed to oxygen excess. The oxidation current of .O(2)(-) were increased plants were grown by high-oxygen environment compared to plants were grown at atmosphere. This experiment was indicated that environmental stress such as hyperoxia induced excess .O(2)(-) and Au wire sensor using iron porphyrin complexes is capable of .O(2)(-) detection in plants under environmental stresses.
Yuasa
Makoto
M
Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science, Noda, Japan. yuasa@rs.noda.tus.ac.jp
Oyaizu
Kenichi
K
Murata
Hidenori
H
Kobayashi
Tomohiro
T
Kobayashi
Chihiro
C
D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D018384:Oxidative Stress / Q000502:physiology*; D010944:Plants / Q000737:chemistry / Q000378:metabolism*; D011166:Porphyrins / Q000737:chemistry*; D013481:Superoxides / Q000032:analysis / Q000378:metabolism
D011166:Porphyrins; D013481:Superoxides; D006046:Gold; D007501:Iron