Tailoring nanoparticle surface chemistry to enhance laser desorption ionization of peptides and proteins.
Gold nanoparticles capped with 4-aminothiophenol have been employed for laser desorption ionization mass spectrometry of biomolecules. We demonstrate that the capped nanoparticles increase ion yields, decrease ion fragmentation, and increase the useful analyte mass range when compared to other nanoparticle systems. These results will allow for further development of nanoparticles with both targeting and enhanced ionization abilities to aid in biomarker screening.
Castellana
Edward T
ET
Department of Chemistry, Texas A&M University, College Station, Texas 77843, USA.
Russell
David H
DH
D000814:Aniline Compounds / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D016503:Drug Delivery Systems / Q000379:methods*; D006046:Gold / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D015336:Molecular Probe Techniques; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013438:Sulfhydryl Compounds; D013499:Surface Properties
D000814:Aniline Compounds; D046911:Macromolecular Substances; D010455:Peptides; D011506:Proteins; D013438:Sulfhydryl Compounds; D006046:Gold; C064316:4-aminothiophenol