title: [Recombinant HEV caspid protein p239 specifically attached on HepG2 cells and blocked the infection of wild-type HEV on liver cells].
abstract: By using Western blot and immunofluorescence assays, the recombinant HEV capsid protein p239 was found specifically attached to the HepG2 cell surface and entered to the cytoplasm with the increase of incubation temperature. Pre-mixture of wild-type HEV with p239 blocked the infectivity of the virus on primary cultured human hepatocytes and HepG2 cells, indicating that p239 and HEV competed the same targeting site on these cells. These data provide evidence that p239 has a similar cell surface structure with wild-type HEV.
authors: National Institute of Diagnostics and Vaccine Development of Infectious Disease, Xiamen University, Xiamen 361005, China.
authors: Ji
authors: J
authors: Miao
authors: Zi-zheng
authors: ZZ
authors: Zheng
authors: Shui-zhen
authors: SZ
authors: He
authors: Ping-guo
authors: PG
authors: Liu
authors: Xiao-cheng
authors: XC
authors: Wu
authors: Yuan-yuan
authors: YY
authors: Sun
authors: Ming
authors: M
authors: Tang
authors: Jun
authors: J
authors: Zhang
authors: Ning-shao
authors: NS
authors: Xia
mesh_terms: D015153:Blotting, Western; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005455:Fluorescent Antibody Technique; D016752:Hepatitis E virus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism / Q000821:virology*; D006801:Humans; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D036022:Capsid Proteins; D011994:Recombinant Proteins