Paramphistomum cervi: antigenic profile of adults as recognized by infected cattle sera.
An antigenic profile of adult Paramphistomum cervi was revealed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting using sera from cattle naturally infected with P. cervi, Fasciola gigantica and strongylids. SDS-PAGE of whole worm extracts exhibited 26 distinct protein bands. Immunoblotting analysis of these proteins showed five major antigenic bands which were recognized by serum of individual cattle naturally infected with P. cervi. These antigenic proteins had molecular weights ranging from 23 to 116kDa. One antigenic protein with a molecular weight of 52kDa exhibited a consistent reaction with sera from all infected cattle. It's diagnostic sensitivity, specificity and accuracy using this test were 100%, 98% and 98.9%, respectively. The positive and negative predictive values were 97.6% and 100%, respectively. This finding suggests that the 52kDa protein may be a diagnostic antigen for paramphistomosis.
Anuracpreeda
Panat
P
Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.
Wanichanon
Chaitip
C
Sobhon
Prasert
P
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D007106:Immune Sera / Q000276:immunology*; D015151:Immunoblotting / Q000662:veterinary; D010250:Paramphistomatidae / Q000276:immunology*; D012680:Sensitivity and Specificity; D016622:Silver Staining / Q000662:veterinary; D014201:Trematode Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*
D000947:Antigens, Helminth; D007106:Immune Sera