Polymerization of the actin-like protein MamK, which is associated with magnetosomes.
The recombinant actin-like protein MamK was purified from Escherichia coli and used as an antigen to generate the anti-MamK antibody. Immunostaining studies showed a linear distribution of MamK in Magnetospirillum magnetotacticum cells and of MamK in association with magnetosomes. Moreover, we demonstrated that MamK polymerizes into filamentous bundles in vitro.
Taoka
Azuma
A
Department of Life Science, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
Asada
Ryuji
R
Wu
Long-Fei
LF
Fukumori
Yoshihiro
Y
D000199:Actins / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism*; D042201:Magnetospirillum / Q000166:cytology* / Q000378:metabolism*; D015388:Organelles / Q000378:metabolism*; D021381:Protein Transport
D000199:Actins; D001426:Bacterial Proteins; D001704:Biopolymers