title: Expression of periostin and its clinicopathological relevance in gastric cancer.
abstract: OBJECTIVE
  To investigate the expression and localization of periostin in gastric cancer and its clinical relevance.
  
  
  METHODS
  Reverse transcriptase polymerase chain reaction was used to measure periostin mRNA expression. Western blotting was carried out to detect periostin protein expression. Immunohistochemistry was performed to localize and quantify the expression of periostin in benign gastric diseases and gastric cancer, and immunostaining results were correlated with gastric cancer pathological stages.
  
  
  RESULTS
  Periostin expression was low at both mRNA and protein levels in normal gastric tissues, but was overexpressed in gastric cancer tissues. Immunohistochemical staining revealed that periostin was overexpressed in primary gastric cancer, as well as in metastatic lymph nodes, but only faint staining was found in benign gastric ulcers. By quantitative analysis of the immunostaining results, periostin expression was increased 2.5-4-fold in gastric cancer, compared to that in benign gastric disease, and there was a trend toward increasing periostin expression with tumor stage.
  
  
  CONCLUSIONS
  This observation demonstrated that periostin was overexpressed in gastric cancer and lymph node metastasis, which suggests that periostin plays an important role in the progression and metastasis of gastric cancer.
authors: Department of General Surgery, Affiliated Zhong-Da Hospital, Southeast University, Nanjing 210009, Jiangsu Province, China. lijunshenghd@126.com
authors: Jun-Sheng
authors: JS
authors: Li
authors: Guang-Wen
authors: GW
authors: Sun
authors: Xiao-Ying
authors: XY
authors: Wei
authors: Wen-Hao
authors: WH
authors: Tang
mesh_terms: D000368:Aged; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D015815:Cell Adhesion Molecules; C512835:POSTN protein, human; D012333:RNA, Messenger