title: Reduction of DNA damage in older healthy adults by Tri E Tocotrienol supplementation.
abstract: OBJECTIVE
  The free radical theory of aging (FRTA) suggests that free radicals are the leading cause of deteriorating physiologic function during senescence. Free radicals attack cellular structures or molecules such as DNA resulting in various modifications to the DNA structures. Accumulation of unrepaired DNA contributes to a variety of disorders associated with the aging process.
  
  
  METHODS
  A randomized, double-blinded placebo-controlled study was undertaken to evaluate the effect of Tri E Tocotrienol on DNA damage. Sixty four subjects 37-78 y old completed the study. A daily dose of 160 mg of Tri E Tocotrienol was given for 6 months. Blood samples were analyzed for DNA damage using comet assay, frequency of sister chromatid exchange (SCE), and chromosome 4 aberrations.
  
  
  RESULTS
  Results showed a significant reduction in DNA damage as measured by comet assay after 3 mo (P < 0.01) and remained low at 6 mo (P < 0.01). The frequency of SCE was also reduced after 6 mo of supplementation (P < 0.05), albeit more markedly in the >50 y-old group (P < 0.01) whereas urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels were significantly reduced (P < 0.05). A strong positive correlation was observed between SCE with age, whereas weak positive correlations were observed in DNA damage and 8-OHdG, which were reduced with supplementation. However, no translocation or a stable insertion was observed in chromosome 4.
  
  
  CONCLUSIONS
  Tri E Tocotrienol supplementation may be beneficial by reducing DNA damage as indicated by a reduction in DNA damage, SCE frequency, and urinary 8-OHdG.
authors: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
authors: Siok-Fong
authors: SF
authors: Chin
authors: Noor Aini Abdul
authors: NA
authors: Hamid
authors: Azian Abdul
authors: AA
authors: Latiff
authors: Zaiton
authors: Z
authors: Zakaria
authors: Musalmah
authors: M
authors: Mazlan
authors: Yasmin Anum Mohd
authors: YA
authors: Yusof
authors: Aminuddin Abdul
authors: AA
authors: Karim
authors: Johari
authors: J
authors: Ibahim
authors: Zalina
authors: Z
authors: Hamid
authors: Wan Zurinah Wan
authors: WZ
authors: Ngah
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000375:Aging; D000975:Antioxidants / Q000494:pharmacology*; D002894:Chromosomes, Human, Pair 4 / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D019587:Dietary Supplements; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species; D012854:Sister Chromatid Exchange / Q000187:drug effects; D024508:Tocotrienols / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D024508:Tocotrienols; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine