MALDI-linear ion trap microprobe MS/MS studies of the effects of dichloroacetate on lipid content of nerve tissue.
The direct analysis of tissue from both the central and peripheral nervous systems of control rats and those administered the potential neurotoxin dichloroacetate (DCA) was investigated using an intermediate-pressure matrix-assisted laser desorption/ionization (IP-MALDI) source coupled to a linear ion trap (LIT) mass spectrometer. The matrix, 2,5-dihydroxybenzoic acid, was applied to the tissue using a novel automated inkjet printer system. The MSn capabilities of the LIT allowed identification of lipids desorbed directly from tissue. A marked decrease is observed in the intensity of lipid ions in spinal cord and sciatic nerve tissues from rats exposed to DCA. The results also demonstrate the rapid, sensitive, and semiquantitative capabilities of this method.
Landgraf
Rachelle R
RR
Department of Chemistry, University of Florida, Gainesville, Florida 32611, USA.
Garrett
Timothy J
TJ
Calcutt
Nigel A
NA
Stacpoole
Peter W
PW
Yost
Richard A
RA
D000818:Animals; D003999:Dichloroacetic Acid / Q000494:pharmacology*; D005260:Female; D008055:Lipids / Q000032:analysis*; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012584:Sciatic Nerve / Q000737:chemistry* / Q000187:drug effects; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013116:Spinal Cord / Q000737:chemistry* / Q000187:drug effects
D008055:Lipids; D003999:Dichloroacetic Acid