title: Whole-genome shotgun sequencing of mitochondria from ancient hair shafts.
abstract: Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.
authors: Centre for Ancient Genetics, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, Denmark.
authors: M Thomas P
authors: MT
authors: Gilbert
authors: Lynn P
authors: LP
authors: Tomsho
authors: Snjezana
authors: S
authors: Rendulic
authors: Michael
authors: M
authors: Packard
authors: Daniela I
authors: DI
authors: Drautz
authors: Andrei
authors: A
authors: Sher
authors: Alexei
authors: A
authors: Tikhonov
authors: Love
authors: L
authors: Dalén
authors: Tatyana
authors: T
authors: Kuznetsova
authors: Pavel
authors: P
authors: Kosintsev
authors: Paula F
authors: PF
authors: Campos
authors: Thomas
authors: T
authors: Higham
authors: Matthew J
authors: MJ
authors: Collins
authors: Andrew S
authors: AS
authors: Wilson
authors: Fyodor
authors: F
authors: Shidlovskiy
authors: Bernard
authors: B
authors: Buigues
authors: Per G P
authors: PG
authors: Ericson
authors: Mietje
authors: M
authors: Germonpré
authors: Anders
authors: A
authors: Götherström
authors: Paola
authors: P
authors: Iacumin
authors: Vladimir
authors: V
authors: Nikolaev
authors: Malgosia
authors: M
authors: Nowak-Kemp
authors: Eske
authors: E
authors: Willerslev
authors: James R
authors: JR
authors: Knight
authors: Gerard P
authors: GP
authors: Irzyk
authors: Clotilde S
authors: CS
authors: Perbost
authors: Karin M
authors: KM
authors: Fredrikson
authors: Timothy T
authors: TT
authors: Harkins
authors: Sharon
authors: S
authors: Sheridan
authors: Webb
authors: W
authors: Miller
authors: Stephan C
authors: SC
authors: Schuster
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000737:chemistry; D004249:DNA Damage; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics / Q000266:history*; D004606:Elephants / Q000235:genetics*; D050259:Genes, Mitochondrial; D016678:Genome; D006197:Hair / Q000737:chemistry / Q000648:ultrastructure; D049690:History, Ancient; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D011309:Preservation, Biological; D017422:Sequence Analysis, DNA; D012800:Siberia; D013696:Temperature
chemical_list: D004272:DNA, Mitochondrial