Osteogenic differentiation effects on rat bone marrow-derived mesenchymal stromal cells by lentivirus-mediated co-transfection of human BMP2 gene and VEGF165 gene.
We proposed a novel combined gene therapy of human vascular endothelial growth factor 165 gene (hVEGF165) and human bone morphogenetic protein 2 gene (hBMP2) for bone regeneration by lentivirus-mediated co-transfection of both genes into rat bone marrow-derived mesenchymal stromal cells (MSCs). Both genes were successfully co-expressed in MSCs confirmed by real-time PCR and ELISA. And the alkaline phosphatase activity of MSCs was significantly augmented by the co-transfection with both genes than any single gene transfection (P < 0.01). These results demonstrated the feasibility of the combined gene therapy by using MSCs lentivirally co-transfected with hVEGF165 and hBMP2 for bone regeneration.
Jiang
Jia
J
Department of Orthopedics, The Sixth Affiliated People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.
Fan
Cun-yi
CY
Zeng
Bing-fang
BF
D000818:Animals; D001854:Bone Marrow Cells; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D008648:Mesoderm / Q000166:cytology*; D010012:Osteogenesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology*; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000235:genetics*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
C525718:BMP2 protein, human; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D016212:Transforming Growth Factor beta; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A