title: Evaluation of lipid peroxidation product, nitrite and antioxidant levels in newly diagnosed and two months follow-up patients with pulmonary tuberculosis.
abstract: This case-control study followed by a longitudinal cohort study was undertaken to evaluate the level of lipid peroxidation product malondialdehyde (MDA) and nitrite as an indirect measurement of nitric oxide vis-à-vis the levels of antioxidants vitamin C and vitamin E in pulmonary tuberculosis. Fifty-six sputum smear-positive cases of pulmonary tuberculosis based on Ziehl-Neelsen (ZN) staining and 50 healthy controls without any systemic disease were included in this study. Thirty-five cases were longitudinally followed up with standard antituberculosis chemotherapy (ATT) for two months. Serum levels of malondiadehyde (MDA), nitrite, and plasma levels of vitamins C and E were measured. The mean serum MDA level was significantly higher (8.1 +/- 1.61 nmoles/ml) in PTB patients before commencement of ATT as compared to healthy controls (3.45 +/- 1.7 nmoles/ml) (p=0.0001) and decreased significantly after 2 months of ATT (3.84 +/- 1.28 nmoles/ml) (p=0.0001). The mean serum nitrite level (47.19 +/- 18.44 micromol/l) was significantly elevated before ATT compared to healthy controls (32.89 +/- 11.94 micromoles/l) and decreased significantly after 2 months of ATT (27.71 +/- 11.97 micromoles/l) (p=0.0001). The mean plasma levels of vitamins C (0.88 +/- 0.33 mg/dl) and E (0.79 +/- 0.24 mg/dl) in PTB patients before commencement of ATT were lower than healthy controls (1.42 +/- 0.38 mg/dl) and (1.35 +/- 0.35 mg/dl), respectively (p=0.001). There was a significant increase in vitamin C levels after 2 months of ATT (1.19 +/- 0.40 mg/dl) compared to before ATT (0.83 +/- 0.31 mg/dl) (p=0.0001), but no significant change in mean plasma vitamin E level before and after 2 months on ATT was found. Elevated malondialdehyde and nitrite levels with concomitant depressed vitamin C and E levels are indicative of lipid peroxidation and oxidative stress. The decrease in levels of malondialdehyde and nitrite with subsequent increase in vitamin C levels after two months of follow-up indicate a good response to treatment with standard ATT. Hence, the extent of oxidative stress in PTB can be evaluated by analyzing lipid peroxidation product, antioxidant and nitric oxide levels.
authors: Department of Biochemistry, B. P. Koirala Institute of Health Sciences, Dharan, Nepal. madhablamsal@yahoo.co.uk
authors: Madhab
authors: M
authors: Lamsal
authors: Narayan
authors: N
authors: Gautam
authors: Narendra
authors: N
authors: Bhatta
authors: Bishamber Dass
authors: BD
authors: Toora
authors: Shymal Kumar
authors: SK
authors: Bhattacharya
authors: Nirmal
authors: N
authors: Baral
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism; D001205:Ascorbic Acid / Q000097:blood / Q000378:metabolism; D015331:Cohort Studies; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008137:Longitudinal Studies; D008297:Male; D008315:Malondialdehyde / Q000032:analysis* / Q000378:metabolism; D008875:Middle Aged; D009390:Nepal; D009569:Nitric Oxide / Q000032:analysis* / Q000378:metabolism; D013183:Sputum; D014397:Tuberculosis, Pulmonary / Q000378:metabolism*; D014810:Vitamin E / Q000097:blood / Q000378:metabolism
chemical_list: D000975:Antioxidants; D014810:Vitamin E; D009569:Nitric Oxide; D008315:Malondialdehyde; D001205:Ascorbic Acid