Maintenance of CD4+ T cell TCR Vbeta repertoire heterogeneity is characteristic of apathogenic SIV infection in non-human primate model of AIDS.
A PCR-based assay was established to screen for potential changes in the heterogeneity of Vbeta TCR expressing CD4+ T cells from rhesus macaques (RM) and sooty mangabeys (SM) prior to and following SIV infection. The rationale for these studies was to determine whether the progressive CD4+ T cell loss in SIV-infected disease susceptible RM and the moderate CD4+ T cell loss in disease resistant SM leads to the depletion of select Vbeta TCR families of CD4+ T cells. Results show that whereas SIV infection leads to the loss of Vbeta TCR heterogeneity in disease susceptible RM, the CD4+ T cells from SM retain their degree of Vbeta TCR heterogeneity, suggesting that the mechanism(s) of SIV induced CD4+ T cell loss maybe distinct in these 2 species and contribute to the differences in the clinical outcome.
Bostik
Pavel
P
Department of Pathology and Laboratory Medicine, Emory University, WMB rm#2337A, 101 Woodruff Circle, Atlanta, GA 30322, USA. Pavel.Bostik@emory.edu
Stephenson
Susan T
ST
Lynch
Rebecca M
RM
Cardona
Adriana
A
Ansari
Aftab A
AA
D000818:Animals; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016665:Cercocebus atys; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004195:Disease Models, Animal; D019674:Genes, T-Cell Receptor beta; D006801:Humans; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000235:genetics / Q000276:immunology* / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000276:immunology* / Q000472:pathogenicity
D017931:DNA Primers; D018076:DNA, Complementary; D016693:Receptors, Antigen, T-Cell, alpha-beta