Removal of estrogenic activity of natural and synthetic hormones from a municipal wastewater: efficiency of horseradish peroxidase and laccase from Trametes versicolor.
Some researches studied the removal of steroid estrogens by enzymatic treatment, however none verified the residual estrogenicity after the enzymatic treatment at environmental conditions. In this study, the residual estrogenic activities of the key natural and synthetic steroid estrogens were investigated following enzymatic treatment with horseradish peroxidase (HRP) and laccase from Trametes versicolor. Synthetic water and municipal wastewater containing environmental concentrations of estrone, 17beta-estradiol, estriol, and 17alpha-ethinylestradiol were treated. Liquid chromatography-mass spectrometry analysis demonstrated that the studied steroid estrogens were completely oxidized in the wastewater reaction mixture after a 1-h treatment with either HRP (8-10 U ml(-1)) or laccase (20 U ml(-1)). Using the recombinant yeast assay, it was also confirmed that both enzymatic treatments were very efficient in removing the estrogenic activity of the studied steroid estrogens. The laccase-catalyzed process seemed to present great advantages over the HRP-catalyzed system for up-scale applications for the treatment of municipal wastewater.
Auriol
Muriel
M
University of Missouri-Rolla, Environmental Research Center of Emerging Contaminants, 220 Butler Carlton Hall, Rolla, MO 65409, USA.
Filali-Meknassi
Youssef
Y
Adams
Craig D
CD
Tyagi
Rajeshwar D
RD
Noguerol
Tania-Noelia
TN
Piña
Benjamin
B
D004967:Estrogens / Q000737:chemistry* / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D042845:Laccase / Q000737:chemistry*; D008923:Missouri; D016877:Oxidants / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D020072:Polyporales / Q000201:enzymology; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000378:metabolism; D018508:Water Purification / Q000379:methods; D015003:Yeasts / Q000235:genetics
D004967:Estrogens; D016877:Oxidants; D011960:Receptors, Estrogen; D014874:Water Pollutants, Chemical; D006861:Hydrogen Peroxide; D042845:Laccase; D006735:Horseradish Peroxidase; D010100:Oxygen