No difference in the skeletal muscle angiogenic response to aerobic exercise training between young and aged men.
Ischaemia-induced skeletal muscle angiogenesis is impaired in aged compared with young mice. In humans, vascular endothelial growth factor (VEGF) mRNA and protein following an acute exercise bout are lower in aged compared with young untrained men. We hypothesized that exercise-induced skeletal muscle angiogenesis would be attenuated in aged compared with young men. In eight aged (mean age: 64 years) and six young (mean age: 25 years) sedentary men, muscle biopsies were obtained from the vastus lateralis prior to (Pre), after 1 week and after 8 weeks of an aerobic exercise training program for the measurement of capillarization and VEGF mRNA. Dialysate VEGF protein collected from the muscle interstitial space was measured at rest and during submaximal exercise at Pre, 1 week and 8 weeks. Exercise training increased capillary contacts (CC) and capillary-to-fibre perimeter exchange index (CFPE) of type I and IIA fibres similarly in young and aged. The CC of type IIA and IIB fibres was lower in aged compared with young independent of training status. Exercise-induced interstitial VEGF protein was lower in aged compared with young independent of training status. In untrained, greater exercise-induced interstitial VEGF protein during exercise was associated with greater type I, IIA and IIB CC. Exercise training increased VEGF mRNA similarly in young and aged. These results demonstrate that the angiogenic response to aerobic exercise training is not altered during the ageing process in humans. In addition, muscular activity-associated increases in interstitial VEGF protein may play an important role in the maintenance of skeletal muscle capillarization across the life span.
Gavin
Timothy P
TP
Department of Exercise and Sport Science, Human Performance Laboratory, East Carolina University, Greenville, NC 27858, USA. gavint@ecu.edu
Ruster
Rebecca S
RS
Carrithers
John A
JA
Zwetsloot
Kevin A
KA
Kraus
Raymond M
RM
Evans
Christopher A
CA
Knapp
Deborah J
DJ
Drew
James L
JL
McCartney
Jennifer S
JS
Garry
Joseph P
JP
Hickner
Robert C
RC
D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology; D002196:Capillaries / Q000254:growth & development / Q000502:physiology; D015444:Exercise / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A