Escherichia coli physiology in Luria-Bertani broth.
Luria-Bertani broth supports Escherichia coli growth to an optical density at 600 nm (OD(600)) of 7. Surprisingly, however, steady-state growth ceases at an OD(600) of 0.3, when the growth rate slows down and cell mass decreases. Growth stops for lack of a utilizable carbon source. The carbon sources for E. coli in Luria-Bertani broth are catabolizable amino acids, not sugars.
Sezonov
Guennadi
G
Unité Biologie Moléculaire du Gène chez les Extrémophiles, Institut Pasteur, 25, rue du Docteur Roux, F-75724 Paris Cedex 15, France. sezonov@pasteur.fr
Joseleau-Petit
Danièle
D
D'Ari
Richard
R
D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D002241:Carbohydrates / Q000737:chemistry; D002244:Carbon / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology*; D005285:Fermentation
D000596:Amino Acids; D002241:Carbohydrates; D003470:Culture Media; D002244:Carbon