title: Intravital two-photon imaging of T-cell priming and tolerance in the lymph node.
abstract: Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of lymph node T cells during either priming or tolerance, in live, anesthetized mice. Intravital imaging of T lymphocytes is a powerful tool to study the cellular orchestration of adaptive immune responses in physiological settings. This method should provide new insights into the regulation of lymphocyte migration and cell-cell interactions in various immunological contexts.
authors: Départment d'Immunologie, Institut Pasteur, Paris, France.
authors: Susanna
authors: S
authors: Celli
authors: Philippe
authors: P
authors: Bousso
mesh_terms: D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D003952:Diagnostic Imaging / Q000379:methods*; D007108:Immune Tolerance / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D018613:Microscopy, Confocal / Q000379:methods; D017785:Photons; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation