A study on pathogenesis of sudden death syndrome in broiler chickens.
Sudden death syndrome (SDS) in fast growing broiler chickens has been recognized as a patho-physiological entity for four decades, but its pathogenesis still remains unknown. More recent investigations provided evidence that link SDS to cardiac arrhythmia, but the mechanism triggering arrhythmogenesis and factors responsible for fatal outcome are poorly understood. In order to understand the chain of events leading to SDS in broilers, the present study focused on putative mechanisms that trigger arrhythmia and mechanisms that predispose the myocardium to fatal arrhythmia. Susceptibility of broilers to cardiac arrhythmia under stress conditions was evaluated using a simulated stress test with epinephrine. Detailed histopathological evaluation of the broiler heart was undertaken to identify structural features that may predispose the myocardium to fatal arrhythmia. The simulated stress challenge revealed that many broilers are highly susceptible to stress induced cardiac arrhythmia. In some broilers the stress challenge induced severe ventricular arrhythmia, and the life threatening nature of this arrhythmia was evidenced by the fact that several birds showing the most severe arrhythmic responses, died suddenly within several days after the stress challenge. Examination of hearts of broilers that died of SDS revealed microscopic lesions in the cardiomyocytes, and widespread changes in the sub-endocardial and mural His-Purkinje system (HPS). Immune staining for Caspase-3 confirmed that numerous Purkinje cells in the left ventricular myocardium from broiler chickens that died of SDS were undergoing apoptosis. The observed lesions suggest that the electrical stability of the myocardium was compromised. Taken together, our findings indicate that stress is a most likely trigger of cardiac arrhythmia in broilers, whereas the pathological changes seen in the myocardium and in the HPS in fast growing broilers provide a very conducive milieu for sustained ventricular arrhythmia. In cases where the electrical stability of the myocardium is compromised, even an episodic arrhythmic event may readily degenerate to catastrophic ventricular fibrillation and sudden death. We conclude that the combination of stress and changes in the cardiomyocytes and HPS are the key requisite features in the pathogenesis of SDS.
Olkowski
A A
AA
Department of Animal and Poultry Science, University of Saskatchewan, 6D34 Agriculture Building, 51 Campus Drive, Saskatoon, SK, Canada. Andrew.Olkowski@usask.ca
Wojnarowicz
C
C
Nain
S
S
Ling
B
B
Alcorn
J M
JM
Laarveld
B
B
D000818:Animals; D001145:Arrhythmias, Cardiac / Q000503:physiopathology / Q000662:veterinary; D002645:Chickens; D003645:Death, Sudden / Q000209:etiology / Q000662:veterinary*; D004837:Epinephrine / Q000494:pharmacology; D009206:Myocardium / Q000473:pathology; D011201:Poultry Diseases / Q000453:epidemiology / Q000209:etiology*; D013312:Stress, Physiological / Q000139:chemically induced
D004837:Epinephrine