title: Low- to high-throughput analysis of telomerase modulators with Telospot.
abstract: We designed a method termed Telospot to discover and characterize telomerase modulators as anticancer drugs or chemical biology tools. Telospot is based on a highly efficient human telomerase expression system and the detection of telomerase DNA reaction products in macroarray format. Telospot offers a highly scalable, cost- and time-effective alternative to presently available telomerase assays, which are limited by the requirement for PCR, telomerase purification or technologies not amenable to high throughput.
authors: Swiss Institute for Experimental Cancer Research, Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland.
authors: Gaël
authors: G
authors: Cristofari
authors: Patrick
authors: P
authors: Reichenbach
authors: Pierre-Olivier
authors: PO
authors: Regamey
authors: Damiano
authors: D
authors: Banfi
authors: Marc
authors: M
authors: Chambon
authors: Gerardo
authors: G
authors: Turcatti
authors: Joachim
authors: J
authors: Lingner
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis*; D006801:Humans; D019098:Telomerase / Q000032:analysis / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; C509186:TERT protein, human; D019098:Telomerase