title: Differential expression of syndecan isoforms during mouse incisor amelogenesis.
abstract: Syndecans are transmembranous heparan sulfate proteoglycans (HSPGs) with covalently attached glycosaminoglycan side-chains located on the cell surface. The mammalian syndecan family is composed of four types of syndecans (syndecan-1 to -4). Syndecans interact with the intracellular cytoskeleton through the cytoplasmic domains of their core proteins and membrane proteins, extracellular enzymes, growth factors, and matrix components, through their heparan-sulfate chains, to regulate developmental processes.Here, as a first step to assess the possible roles of syndecan proteins in amelogenesis, we examined the expression patterns of all syndecan isoforms in continuously growing mouse incisors, in which we can overview major differentiation stages of amelogenesis at a glance. Understanding the expression domain of each syndecan isoform during specific developmental stages seems useful for investigating their physiological roles in amelogenesis. Immunohistochemical analysis of syndecan core proteins in the lower incisors from postnatal day 1 mice revealed spatially and temporally specific expression patterns, with syndecan-1 expressed in undifferentiated epithelial and mesenchymal cells, and syndecan-2, -3, and -4 in more differentiated cells. These findings suggest that each syndecan isoform functions distinctly during the amelogenesis of the incisors of mice.
authors: Department of Molecular Biology, The University of Tokushima Graduate School, Tokushima, Japan.
authors: Taro
authors: T
authors: Muto
authors: Keiko
authors: K
authors: Miyoshi
authors: Seiichi
authors: S
authors: Munesue
authors: Hiroshi
authors: H
authors: Nakada
authors: Minoru
authors: M
authors: Okayama
authors: Takashi
authors: T
authors: Matsuo
authors: Takafumi
authors: T
authors: Noma
mesh_terms: D000566:Amelogenesis / Q000235:genetics / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D007180:Incisor / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D053667:Syndecans / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D020033:Protein Isoforms; D053667:Syndecans