Identification and expression analysis of novel LAGE-1 alleles with single nucleotide polymorphisms in cancer patients.
OBJECTIVE
The purpose of this study is to identify single nucleotide polymorphisms (SNPs) in the coding region alleles of the X chromosomal LAGE-1 gene, and investigate the frequency of such SNPs in both cancer patients and healthy controls, and thus determine the potential significance of these SNPs with respect to cancer vaccine therapy.


METHODS
In this study, different mRNAs transcribed from the LAGE-1 gene were identified by RT-PCR from healthy donors and cancer patients samples.


RESULTS
A new LAGE-1 allele containing three coding region SNPs (69A/G, 317C/G, and 397T/G) were identified. The allele is highly expressed as the LAGE-1a mRNA variant AY679089 in some of the cancer patients. The three SNPs altered the LAGE-1 gene sequence to that of NYESO-1 at both the nucleotide and amino acid level.


CONCLUSIONS
There is a high frequency of the LAGE-1 gene allele with SNPs in coding regions in cancer patients. There was a clear relationship between the variant AY679089 and gastric cancer. The SNPs may lead to accelerated progress of poorly differentiated gastric cancer. The SNPs found in these alleles may also alter the immunological characteristics of LAGE-1a and should be taken into account if this antigen is adopted as a cancer vaccine component.
Shao
Yi
Y
Department of liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, CAMS & PUMC, Beijing 100730, China.
Sun
Zhen-Yuan
ZY
Sun
Shi-Wei
SW
Zhao
Yi
Y
Sin
Wan Yee
WY
Yuan
Yan-Hua
YH
Simpson
Andrew J
AJ
Old
Lloyd J
LJ
Sang
Xin-Ting
XT
Mao
Yi-Lei
YL
Xie
Yong
Y
Huang
Jie-Fu
JF
Zhao
Hai-Tao
HT
D000483:Alleles; D000951:Antigens, Neoplasm / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D013274:Stomach Neoplasms / Q000235:genetics
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; C510162:CTAG2 protein, human