Salvianolic acid B, an antioxidant from Salvia miltiorrhiza, prevents 6-hydroxydopamine induced apoptosis in SH-SY5Y cells.
Oxidative stress caused by dopamine may play an important role in the pathogenesis of Parkinson's disease. Salvianolic acid B is an antioxidant derived from the Chinese herb, Salvia miltiorrhiza. In this study, we investigated the neuroprotective effect of salvianolic acid B against 6-hydroxydopamine-induced cell death in human neuroblastoma SH-SY5Y cells. Pretreatment of SH-SY5Y cells with salvianolic acid B significantly reduced 6-hydroxydopamine-induced generation of reactive oxygen species, and prevented 6-hydroxydopamine-induced increases in intracellular calcium. Our data demonstrated that 6-hydroxydopamine-induced apoptosis was reversed by salvianolic acid B treatment. Salvianolic acid B reduced the 6-hydroxydopamine-induced increase of caspase-3 activity, and reduced cytochrome C translocation into the cytosol from mitochondria. The 6-hydroxydopamine-induced decrease in the Bcl-x/Bax ratio was prevented by salvianolic acid B. Additionally, salvianolic acid B decreased the activation of extracellular signal-regulated kinase and induced the activation of 6-hydroxydopamine-suppressed protein kinase C. These results indicate that the protective function of salvianolic acid B is dependent upon its antioxidative potential. Our results strongly suggest that salvianolic acid B may be effective in treating neurodegenerative diseases associated with oxidative stress.
Tian
Lin-Lin
LL
Laboratory of Biotechnology, Beijing Institute of Radiation Medicine, 27# Taiping Road, Haidian District, Beijing 100850, PR China.
Wang
Xue-Jun
XJ
Sun
Yu-Ning
YN
Li
Chun-Rong
CR
Xing
Ya-Ling
YL
Zhao
Hai-Bao
HB
Duan
Ming
M
Zhou
Zhe
Z
Wang
Sheng-Qi
SQ
D018663:Adrenergic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001572:Benzofurans / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D045304:Cytochromes c / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D016627:Oxidopamine / Q000378:metabolism / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D027561:Salvia miltiorrhiza; D013565:Sympatholytics / Q000494:pharmacology; D053630:Depsides
D018663:Adrenergic Agents; D000975:Antioxidants; D051017:Apoptosis Regulatory Proteins; D001572:Benzofurans; D002118:Calcium; D053148:Caspase 3; D045304:Cytochromes c; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D016627:Oxidopamine; D011493:Protein Kinase C; D017382:Reactive Oxygen Species; D013565:Sympatholytics; C076944:salvianolic acid B; D053630:Depsides