The influence of the pulsatility of the blood flow on the extent of platelet adhesion.
A new improved flow system was developed to study the influence of blood flow pulsatility on platelet adhesion on adhesive proteins and bio-medical materials. The pulsatility was introduced by changing the shear rate every 15 s in blood that was aspirated through a perfusion chamber by a syringe pump. The advantage of this new system is that it avoids system related platelet activation. At steady low shear rate (300/s) after 5 min a collagen type III surface was covered for 24.2+/-3.8% with platelets. At steady high shear rate (1300/s) platelet coverage to collagen was 48.8+/-6.8%. When pulsatility was introduced by changing the shear rate was every 15 s form 300/s to 1300/s and vice-versa, platelet coverage after 5 min was increased to 60.4+/-4.0% (p<0.001). After 5 min perfusion samples were taken from the perfusate and the extent of platelet activation was measured. The significant difference in surface expression of P-selectin on platelets is only seen when comparing pulse flow with control (no flow). We concluded that a significant increase in platelet activation during blood pulsatile flow compared with steady flow, which results in an increased platelet adhesion to collagen.
Zhao
Xiao Min
XM
College of Medicine, Shandong University, Jinan, China.
Wu
Ya Ping
YP
Cai
Hong Xin
HX
Wei
Ran
R
Lisman
Ton
T
Han
Ji Ju
JJ
Xia
Zuo Li
ZL
de Groot
Philip G
PG
D001783:Blood Flow Velocity; D001792:Blood Platelets / Q000737:chemistry / Q000502:physiology*; D024061:Collagen Type III / Q000737:chemistry / Q000378:metabolism; D018056:Hemorheology / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008955:Models, Cardiovascular; D019007:P-Selectin / Q000032:analysis; D010973:Platelet Adhesiveness / Q000502:physiology*; D011673:Pulsatile Flow; D012016:Reference Values; D012680:Sensitivity and Specificity; D013499:Surface Properties
D024061:Collagen Type III; D019007:P-Selectin