HindIII polymorphism in carrier detection of hemophilia A in India: a new primer design.
HindIII polymorphism studies were done in 67 north Indian families with hemophilia A using a new primer sequence and comparing results with the standard protocol. Amplified segment was 608 bp in size. Genetically positive hemizygous males (+) and homozygous females (+/+) showed 427, 100, and 81 size fragments, whereas heterozygous females (+/-) had 427, 181, 100, and 81-bp segments. Genotypically negative hemizygous (-) males and homozygous females (-/-) show that 427-bp and 181-bp fragments dimer formation was minimal and results matched with standard protocol in 20 cases where both polymerase chain reactions were run. The frequency of positive allele for HindIII polymorphism in 67 families was 0.38 and heterozygosity in terms of informativity was 0.46.
Raza
Syed Tasleem
ST
Genetics Laboratory, Department of Pathology, King George's Medical University, Lucknow, India.
Husain
Nuzhat
N
Bhatt
Anant Narayan
AN
Avneesh
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D015247:Deoxyribonuclease HindIII; D005169:Factor VIII / Q000235:genetics*; D005260:Female; D006580:Genetic Carrier Screening / Q000379:methods*; D006467:Hemophilia A / Q000235:genetics*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007194:India; D008297:Male; D012150:Polymorphism, Restriction Fragment Length
D017931:DNA Primers; D005169:Factor VIII; D015247:Deoxyribonuclease HindIII