Ostreae Testa prevent ovariectomy-induced bone loss in mice by osteoblast activations.
OBJECTIVE
The anti-osteoporotic effect of heated powder of Ostreae Testa (hPOT), a powder of oyster shell-Ostrea gigas Thumb (Ostrediae) was observed in vitro and in vivo.


METHODS
The effects on proliferation and alkaline phosphatase activity of primary osteoblasts, bone nodule formation, pit formation of osteoclasts and osteoclastogenesis were observed in vitro, and to observe the in vivo efficacy hPOT was orally administered once a day for 28 days to bilateral ovariectomy-induced osteoporosis mice at 125, 250 and 500mg/kg (of body weight).


RESULTS
Although hPOT did not influence the pit formation and the number of multinucleated osteoclast-like cells, osteoclastogenesis, it enhanced the proliferation of primary osteoblasts, differentiation (ALP activity) and bone nodule formation of osteoblast in vitro. The estrogen-deficient osteoporotic changes were dramatically prevented by hPOT treatment except for osteoclasts/bone perimeter.


CONCLUSIONS
In conclusion, hPOT prevents OVX-induced osteoporosis through osteoblasts activation effects.
Han
Sang Yeob
SY
College of Oriental Medicine, Daegu Haany University, Gyeongsan 712-715, Republic of Korea.
Lee
Jong Rok
JR
Kwon
Young Kyu
YK
Jo
Mi Jeong
MJ
Park
Sook Jahr
SJ
Kim
Sang Chan
SC
Lee
Hyeung Sik
HS
Ku
Sae Kwang
SK
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects / Q000473:pathology; D008516:Medicine, Chinese Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D010024:Osteoporosis / Q000517:prevention & control*; D049895:Ostrea; D010052:Ovariectomy / Q000009:adverse effects*; D011208:Powders
D011208:Powders; D000469:Alkaline Phosphatase