Spontaneously immortalized T lymphocytes from Nijmegen Breakage Syndrome patients display phenotypes typical for lymphoma cells.
We found that the peripheral T lymphocytes from four of eight patients with the lymphoma predisposing Nijmegen Breakage Syndrome (NBS) acquired an unlimited growth potential following in vitro mitogen stimulation and subsequent interleukin-2-dependent propagation. The immortal T cell lines revealed morphological and other features typical for anaplastic large cell lymphoma (ALCL). In addition, multiple copies of ALK, but with no ALK gene rearrangements were found in a subpopulation of cells of one of the immortalized lines. These cell lines may be useful for the in vitro elucidation of mechanisms involved in the development of ALCL.
Siwicki
Jan Konrad
JK
Department of Immunology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, 02-781 Warsaw, Roentgena 5, Poland. jksiwicki@coi.waw.pl
Rymkiewicz
Grzegorz
G
Błachnio
Katarzyna
K
Rygier
Jolanta
J
Kuźniar
Piotr
P
Płoski
Rafał
R
Janusz
Aleksandra
A
Skurzak
Henryk
H
Chrzanowska
Krystyna
K
Steffen
Jan
J
D000077548:Anaplastic Lymphoma Kinase; D002461:Cell Line, Transformed / Q000473:pathology; D002478:Cells, Cultured; D005434:Flow Cytometry; D015321:Gene Rearrangement; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D017728:Lymphoma, Large-Cell, Anaplastic / Q000235:genetics / Q000473:pathology*; D049932:Nijmegen Breakage Syndrome / Q000473:pathology*; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D020794:Receptor Protein-Tyrosine Kinases; D013601:T-Lymphocytes / Q000473:pathology*
C000626173:ALK protein, human; D000077548:Anaplastic Lymphoma Kinase; D011505:Protein-Tyrosine Kinases; D020794:Receptor Protein-Tyrosine Kinases