A novel agar medium to detect hydrogen peroxide-producing bacteria based on the prussian blue-forming reaction.
The classic method for H(2)O(2) detection involving Prussian blue formation was adapted to formulate a novel agar medium that makes possible in situ detection of H(2)O(2) produced by bacteria. Using this medium, colonies of H(2)O(2)-producing species including Streptococcus pyogenes were easily identified by the appearance of blue halos. The utility of the medium was further illustrated by its successful application to the isolation of H(2)O(2)-producing mutants from a non-H(2)O(2)-producing stain of S. pyogenes.
Saito
Mitsumasa
M
Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, Maidashi, Fukuoka, Japan.
Seki
Masanori
M
Iida
Ken-Ichiro
K
Nakayama
Hiroaki
H
Yoshida
Shin-Ichi
S
D000362:Agar; D001431:Bacteriological Techniques; D002863:Chromogenic Compounds; D003470:Culture Media; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism*; D011540:Prussian Blue Reaction / Q000379:methods*; D013290:Streptococcal Infections / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism
D002863:Chromogenic Compounds; D003470:Culture Media; D000362:Agar; D006861:Hydrogen Peroxide