Comparison of universal detectors for high-temperature micro liquid chromatography.
This study compares, through micro high-temperature liquid chromatography (microHTLC), three commercial universal detectors that allow a direct detection of lipids. The detectors are: the charged aerosol detector (CAD), the evaporative light-scattering detector (ELSD) and the ion trap mass spectrometer with atmospheric pressure chemical ionization (APCI) and electrospray ionization (ESI) sources (APCI-MS and ESI-MS). This study shows the feasibility to use the high temperature with these detectors and hybrid behavior between concentration and mass flow rate detector in microHTLC. The detectors were compared in terms of response intensity, linearity and limit of detection for different high temperatures. The charged aerosol detector shows a linear response from 5 to 500 microg/mL and the correlation coefficients (r(2)) obtained for squalene, cholesterol and ceramide IIIB exceed 0.99.
Hazotte
A
A
Groupe de Chimie Analytique de Paris-Sud, EA 4041, IFR 141, School of Pharmacy, Univ. Paris-Sud, F-92296 Châtenay Malabry, France.
Libong
D
D
Matoga
M
M
Chaminade
P
P
D000336:Aerosols / Q000737:chemistry*; D002518:Ceramides / Q000032:analysis; D002784:Cholesterol / Q000032:analysis; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D006358:Hot Temperature; D008027:Light; D008055:Lipids / Q000032:analysis*; D044085:Microfluidics / Q000379:methods*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D013053:Spectrophotometry / Q000295:instrumentation* / Q000379:methods; D013185:Squalene / Q000032:analysis; D013696:Temperature
D000336:Aerosols; D002518:Ceramides; D008055:Lipids; D012997:Solvents; D013185:Squalene; D002784:Cholesterol