title: Phenotypic characterization of pore mutants of the Vibrio cholerae porin OmpU.
abstract: General-diffusion porins form large beta-barrel channels that control the permeability of the outer membrane of gram-negative bacteria to nutrients, some antibiotics, and external signals. Here, we have analyzed the effects of mutations in the OmpU porin of Vibrio cholerae at conserved residues that are known to affect pore properties in the Escherichia coli porins OmpF and OmpC. Various phenotypes were investigated, including sensitivity to beta-lactam antibiotics, growth on large sugars, and sensitivity to and biofilm induction by sodium deoxycholate, a major bile component that acts as an external signal for multiple cellular responses of this intestinal pathogen. Overall, our results indicate that specific residues play different roles in controlling the passage of various compounds. Mutations of barrel wall arginine residues that protrude in the pore affect pore size and growth in the presence of large sugars or sodium deoxycholate. Sensitivity to large cephalosporins is mostly affected by D116, located on the L3 loop, whose homolog in E. coli, OmpF, is a known binding determinant for these drugs. L3 loop residues also affect biofilm induction. The results are interpreted in terms of a homology model based on the structures of E. coli porins.
authors: Department of Biology and Biochemistry, University of Houston, 369 Science and Research Building II, Houston, TX 77204-5001, USA. adelcour@uh.edu
authors: Melissa
authors: M
authors: Pagel
authors: Valérie
authors: V
authors: Simonet
authors: Jie
authors: J
authors: Li
authors: Mathilde
authors: M
authors: Lallemand
authors: Brian
authors: B
authors: Lauman
authors: Anne H
authors: AH
authors: Delcour
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D018441:Biofilms; D003505:Cyclodextrins; D003840:Deoxycholic Acid; D024881:Drug Resistance, Bacterial; D015964:Gene Expression Regulation, Bacterial; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D011134:Polysaccharides / Q000378:metabolism; D011487:Protein Conformation; D014734:Vibrio cholerae / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D003505:Cyclodextrins; C096349:OmpU protein, Vibrio cholerae; D011134:Polysaccharides; D003840:Deoxycholic Acid; C008315:maltodextrin