title: Incidence and characterization of diarrheal enterotoxins of fecal Bacillus cereus isolates associated with diarrhea.
abstract: A total of 490 stool specimens were collected from patients with diarrhea and healthy controls without diarrhea to investigate the incidence of Bacillus cereus and its enterotoxins. B. cereus was found more significant in stools of persons with diarrhea than without diarrhea (9.5% versus 1.8%, P < 0.05), and was also detected more frequent but not significant in individuals aged > or =1 year and in adults than in children aged <1 year (11% and 8% versus 7.8%, P > 0.05). The hemolytic enterotoxin HBL genes of B. cereus isolates (hblA, hblC, hblD) were detected in 58%, 58%, and 68%, respectively, whereas the nonhemolytic enterotoxin NHE genes (nheA, nheB, nheC) were detected more frequent in 71.%, 84%, and 90% of the isolates, respectively. This study suggests that B. cereus isolates harboring 1 or more enterotoxin gene(s) can be a potential cause of diarrhea in Jordanian population.
authors: Department of Biological Sciences, Faculty of Science, University of Jordan, Amman 11942, Jordan.
authors: Mariam Saleh
authors: MS
authors: Al-Khatib
authors: Hala
authors: H
authors: Khyami-Horani
authors: Eman
authors: E
authors: Badran
authors: Asem A
authors: AA
authors: Shehabi
mesh_terms: D000293:Adolescent; D000328:Adult; D001409:Bacillus cereus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism* / Q000472:pathogenicity; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology* / Q000382:microbiology; D004768:Enterotoxins / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D005243:Feces / Q000382:microbiology*; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007597:Jordan / Q000453:epidemiology; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D015995:Prevalence
chemical_list: D004768:Enterotoxins; D006460:Hemolysin Proteins; C040792:enterotoxin, Bacillus cereus