title: Construction of a flocculating yeast for fuel ethanol production.
abstract: The expression vector pYX212 harboring FLO1 gene and kanMX gene was transformed into Saccharomyces. cerevisiae ZWA46. The transformant, ZWA46-F2, showed strong and stable flocculation ability during 20 serial batch cultivations. The flocculation onset of the strain is in the early stationary growth phase, not coincident with the glucose depletion in the culture medium. The flocculation ability of the transformant showed no difference with the initial pH ranging from 3.5 to 6.0. Furthermore, the ethanol concentration and other properties of the transformant strain ZWA46-F2 were similar to those of the wild-type strain ZWA46.
authors: Research Center of Industrial Microorganisms, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China.
authors: Fu-Zhuan
authors: FZ
authors: Wang
authors: Wei
authors: W
authors: Shen
authors: Zhi-Ming
authors: ZM
authors: Rao
authors: Hui-Ying
authors: HY
authors: Fang
authors: Xiao-Bei
authors: XB
authors: Zhan
authors: Jian
authors: J
authors: Zhuge
mesh_terms: D001674:Bioelectric Energy Sources; D003001:Cloning, Molecular; D004736:Energy-Generating Resources; D000431:Ethanol / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D008565:Membrane Proteins / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity
chemical_list: C081721:FL01 protein, S cerevisiae; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol