[The expression of ubiquitin in the healing process of incised wounds of rat's skin and its significance to forensic medicine].
OBJECTIVE
To evaluate the changes of ubiquitin expression in incised wounds of the rat skin.


METHODS
The testing rat groups were subjected to incised skin wounds, with normal rat skin used as control. The expression level of ubiquitin was assessed using immunohistochemistry and imaging analysis technique on skin samples taken at 1, 3, 6, 12h, and on day 1, 3, 6, 10, and 14d after injury.


RESULTS
The expression level of ubiquitin was low in the skin of normal control group. Increased level of ubiquitin expression could be observed 1 h after injury. The expression level of ubiquitin reached its peak on day 6 and started to decline on day 10, and then returned to its normal level on day 14 d after injury.


CONCLUSIONS
Ubiquitin may serve as a potentially useful marker for forensic determination of the skin wound age.
Zeng
Yi
Y
Department of Forensic Medicine, Chongqing university of Medical Sciences, Chongqing 400016, China.
Deng
Shi-xiong
SX
D000818:Animals; D005260:Female; D005554:Forensic Medicine; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries* / Q000378:metabolism*; D013194:Staining and Labeling; D013997:Time Factors; D025801:Ubiquitin / Q000378:metabolism*; D014945:Wound Healing; D014947:Wounds and Injuries / Q000378:metabolism / Q000473:pathology
D025801:Ubiquitin