title: A new pyrrole alkaloid isolated from Arum palaestinum Boiss. and its biological activities.
abstract: The phytochemical analysis of the ethyl acetate fraction of Arum palaestinum Boiss. (Araceae) led to the isolation and identification of a new polyhydroxy alkaloid compound; (S)-3,4,5-trihydroxy-1 H-pyrrol-2(5H)-one (1), and other five known compounds; caffeic acid (2), isoorientin (3), luteolin (4) and vicenin 11 (5), as well as the rare compound 3,6,8-trimethoxy, 5,7,3',4'-tetrahydroxy flavone (6). The structural elucidations of all the compounds were based on spectroscopic data (1H- and 13C-NMR, DEPT, HSQC, HMBC and NOE difference techniques) and comparison with literature data. Investigation of the antioxidant activity of the ethyl acetate fraction indicated its strong scavenging capacity for 1,1 -diphenyl-2-picrylhydrazyl (DPPH) radicals (SC50 3.1+/-0.82 microg/mL). Moreover, the treatment of different human cancer cell lines with the ethyl acetate fraction led to dose-dependant suppression in the proliferation of both breast carcinoma cells (MCF-7; IC50 59.09+/-4.1 microg/mL) and lymphoblastic leukemia cells (1301; IC50 53.1+/-2.9 microg/mL); however, it was found to have no effect on the growth of hepatocellular carcinoma cells (Hep G2).
authors: College of Forest Science, Kookmin University, Seoul, Korea.
authors: S K
authors: SK
authors: El-Desouky
authors: Ki Hun
authors: KH
authors: Kim
authors: Shi Young
authors: SY
authors: Ryu
authors: Ahmad Farouk
authors: AF
authors: Eweas
authors: Amira M
authors: AM
authors: Gamal-Eldeen
authors: Young-Kyoon
authors: YK
authors: Kim
mesh_terms: D000085:Acetates / Q000737:chemistry; D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology; D031058:Arum / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D011758:Pyrroles / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000085:Acetates; D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D016166:Free Radical Scavengers; D010936:Plant Extracts; D011758:Pyrroles; C007650:ethyl acetate