Cell adhesion by aqueous extract of human placenta used as wound healer.
Aqueous extract of human placenta, used as wound healer, has shown significant cell adhesion property on mouse peritoneal macrophages and P388D1 cultured macrophage cell line. This property was offered primarily by fibronectin type III like peptide present in the extract and is comparable to fibronectin on a molar basis. The peptide induce adhesion of cell through cell surface receptors having K(d) = 2.8 +/- 0.9 x 10(-5) M suggesting weak binding. This is in support of integrins receptors that typically exhibit low affinities. Cell adhesion was partially inhibited by Arg-Gly-Asp (RGD) peptide, anti-beta1 integrin suggesting that integrin beta1 receptors have roles to play in the process.
Nath
Sangeeta
S
Division of Structural Biology and Bioinformatics, Indian Institute of Chemical Biology, 4, Raja S C Mullick Road, Jadavpur, Kolkata 700 032, India.
Bhattacharyya
Debasish
D
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D005260:Female; D005353:Fibronectins / Q000494:pharmacology; D006801:Humans; D019012:Integrin beta1 / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009842:Oligopeptides / Q000494:pharmacology; D010924:Placental Extracts / Q000494:pharmacology*; D014867:Water / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects* / Q000276:immunology
D005353:Fibronectins; D019012:Integrin beta1; D009842:Oligopeptides; D010924:Placental Extracts; D014867:Water; C047981:arginyl-glycyl-aspartic acid