title: Changes in regional long-term oxidative metabolism induced by partial serotonergic denervation and chronic variable stress in rat brain.
abstract: Stressful experiences and genetic predisposition have both independent and interactive contributions to the development of depression. The serotonergic system is involved in the development of depression, and administration of neurotoxins that specifically compromise its function leads to symptoms of affective disorders. In order to find out which brain regions are most affected by stress, partial serotonergic denervation and their combination, chronic variable stress (CVS) was applied for 3 week. Serotonergic denervation was elicited by parachloroampetamine (PCA, 2mg/kg), and cytochrome oxidase histochemistry was used to characterize the long-term levels of neuronal oxidative energy metabolism. PCA pretreatment blocked the increase in oxidative activity in chronically stressed rats in medial preoptic area, cortical and medial amygdala. PCA raised oxidative activity compared to control animals in substantia nigra and ventrolateral division of laterodorsal thalamus. CVS reduced the oxidative activity induced by PCA in suprachiasmatic hypothalamus, anteroventral thalamus, hippocampal CA3 region and cortical amygdala. In the dorsal part of the anterior olfactory nucleus chronic stress blocked the decrease in oxidative activity evoked by PCA. Conclusively, partial serotonergic denervation with PCA and chronic variable stress both had independent effects on long-term energy metabolism in several rat brain structures, tending to increase it. However, partial serotonergic denervation by parachloroampetamine and chronic variable stress had in many brain regions an interactive effect on energy metabolism, each factor reducing the effect of the other, which could reflect the weakening of adaptive mechanisms.
authors: Department of Psychology, Centre of Behavioural and Health Sciences, University of Tartu, Tiigi 78, 50410 Tartu, Estonia.
authors: Margus
authors: M
authors: Kanarik
authors: Denis
authors: D
authors: Matrov
authors: Kadri
authors: K
authors: Kõiv
authors: Marika
authors: M
authors: Eller
authors: Margus
authors: M
authors: Tõnissaar
authors: Jaanus
authors: J
authors: Harro
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism* / Q000503:physiopathology; D002908:Chronic Disease; D003714:Denervation; D003866:Depressive Disorder / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D004195:Disease Models, Animal; D003576:Electron Transport Complex IV / Q000378:metabolism; D004734:Energy Metabolism / Q000502:physiology*; D008297:Male; D009434:Neural Pathways / Q000033:anatomy & histology / Q000378:metabolism / Q000503:physiopathology; D010085:Oxidative Phosphorylation; D017729:Presynaptic Terminals / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism*; D018490:Serotonin Agents / Q000494:pharmacology; D013315:Stress, Psychological / Q000150:complications / Q000378:metabolism* / Q000503:physiopathology; D009435:Synaptic Transmission / Q000502:physiology; D013995:Time; D010133:p-Chloroamphetamine / Q000494:pharmacology
chemical_list: D018490:Serotonin Agents; D012701:Serotonin; D010133:p-Chloroamphetamine; D003576:Electron Transport Complex IV