title: Rat organic solute carrier protein 1 (rOscp1) mediated the transport of organic solutes in Xenopus laevis oocytes: isolation and pharmacological characterization of rOscp1.
abstract: Rat organic solute carrier protein 1 (rOscp1) was isolated from a rat testis cDNA library. Isolated rOscp1 cDNA consisted of 1089 base pairs that encoded a 363-amino acid protein, and the amino acid sequence was 88% and 93% identical to that of human OSCP1 (hOSCP1) and mouse Oscp1 (mOscp1), respectively. The message for rOscp1 is highly detected in rat testis. When expressed in X. oocytes, rOscp1 mediated the high affinity transport of p-aminohippurate (PAH) with a Km value of 15.7+/-1.9 microM, and rOscp1-mediated organic solutes were exhibited in time- and Na+-independent manners. rOscp1 also transported various structurally heterogenous compounds such as testosterone, dehydroepiandrosterone sulfate (DHEA-S), and taurocholate with some differences in substrate specificity compared with hOSCP1. Immunohistochemical analysis revealed that the rOscp1 protein is localized in the basal membrane side of Sertoli cells as observed in mouse testis [Kobayashi et al., 2007; Kobayashi, Y., Tsuchiya, A., Hayashi, T., Kohyama, N., Ohbayashi, M., Yamamoto, T., 2007. Isolation and characterization of polyspecific mouse organic solute carrier protein 1 (mOscp1). Drug Metabolism and Disposition 35 (7), 1239-1245]. Thus, the present results indicate that a newly isolated cDNA clone, rOscp1, is a polyspecific organic solute carrier protein with some differences in substrate specificity compared with human and mouse OSCP1.
authors: Department of Surgery, School of Medicine, Showa University Fujigaoka Hospital,1-30 Fujigaoka, Aoba-ku, Yokohama-shi, Kanagawa 227-8501, Japan.
authors: Hisanori
authors: H
authors: Izuno
authors: Yasuna
authors: Y
authors: Kobayashi
authors: Yutaka
authors: Y
authors: Sanada
authors: Daisuke
authors: D
authors: Nihei
authors: Masako
authors: M
authors: Suzuki
authors: Noriko
authors: N
authors: Kohyama
authors: Masayuki
authors: M
authors: Ohbayashi
authors: Toshinori
authors: T
authors: Yamamoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism*; D009928:Organ Specificity; D004364:Pharmaceutical Preparations / Q000378:metabolism; D010802:Phylogeny; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013737:Testis / Q000378:metabolism*; D014982:Xenopus laevis; D010130:p-Aminohippuric Acid / Q000378:metabolism*
chemical_list: D026901:Membrane Transport Proteins; C524686:Oscp1 protein, rat; D004364:Pharmaceutical Preparations; D010130:p-Aminohippuric Acid