Preliminary X-ray crystallographic studies of a Lys49-phospholipase A2 homologue from Bothrops pirajai venom complexed with p-bromo-phenacyl bromide and alpha-tocopherol inhibitors.
PrTX-I, a non-catalytic and myotoxic Lys49-PLA(2) from Bothrops pirajai venom has been crystallized alone and in complex with bromophenacyl bromide (BPB), alpha tocopherol and alpha tocopherol acetate inhibitors. These crystals have shown to diffract X-rays between 2.34 and 1.65 A resolution. All complexes crystals are isomorphous and belong to the space group P2(1) whereas native PrTX-I crystals belong to the P3(1)21.
Dos Santos
Juliana I
JI
Departamento de Física e Biofísica, Instituto de Biociências, UNESP, C. P. 510, CEP 18618-000, Botucatu-SP, Brazil.
Marchi-Salvador
Daniela P
DP
Fernandes
Carlos A H
CA
Silveira
Lucas B
LB
Soares
Andreimar M
AM
Fontes
Marcos R M
MR
D000818:Animals; D017837:Bothrops; D001965:Bromides / Q000494:pharmacology*; D003435:Crotalid Venoms / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008239:Lysine / Q000737:chemistry*; D008958:Models, Molecular; D010741:Phospholipases A / Q000737:chemistry*; D011487:Protein Conformation; D024502:alpha-Tocopherol / Q000494:pharmacology*
D001965:Bromides; D003435:Crotalid Venoms; D004791:Enzyme Inhibitors; D010741:Phospholipases A; D024502:alpha-Tocopherol; D008239:Lysine