Novel mating type-dependent transcripts at the mating type locus in Magnaporthe oryzae.
The mating type locus (MAT1) of Magnaporthe oryzae has similar structural organization to MAT in other ascomycetes and encodes the mating type genes MAT1-1-1 with an alpha-box motif and MAT1-2-1 with an HMG-box motif in the MAT1-1 and MAT1-2 idiomorphs, respectively. Sequence and expression analyses of the MAT1 locus indicated a second open reading frame (ORF), MAT1-1-2, in the MAT1-1 idiomorph, and novel mating-type dependent ORFs (MAT1-1-3 and MAT1-2-2) at the locus. The MAT1-1-3 ORF initiated within the MAT1-1 idiomorph while the MAT1-2-2 ORF initiated at the border of the MAT1-2 idiomorph with both ORFs sharing most of their reading frames in the MAT1 flanking region. This suggests that the encoded proteins (MAT1-1-3 and MAT1-2-2) should be similar in their primary structures but can be distinguished by distinct N-termini with amino acids of 1 and 32, respectively, in each mating type. A CT dinucleotide repeat, (CT)n, present in the upstream region of MAT1-1-3, was polymorphic among the isolates.
Kanamori
Masaki
M
Laboratory of Plant Pathology, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Tokyo 183-8509, Japan.
Kato
Hana
H
Yasuda
Nobuko
N
Koizumi
Shinzo
S
Peever
Tobin L
TL
Kamakura
Takashi
T
Teraoka
Tohru
T
Arie
Tsutomu
T
D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D020029:Base Pairing; D001483:Base Sequence; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D018900:Dinucleotide Repeats; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D024742:HMG-Box Domains; D020082:Magnaporthe / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
D020121:5' Untranslated Regions; D017931:DNA Primers; D004271:DNA, Fungal; D005656:Fungal Proteins; D012333:RNA, Messenger