Transiently, paralleled upregulation of arginase and nitric oxide synthase and the effect of both enzymes on the pathology of asthma.
Changes in the expression of arginase and their association with nitrosative stress were investigated using an asthmatic model previously established in NC/Nga mice with mite extract. Mite crude extract (100 microg/day) from Dermatophagoides farinae was administered intranasally for 5 consecutive days (day 0-4), and a single challenge was performed on day 11. On day 12, upregulation of the mRNA expression of inducible types of nitric oxide synthase (iNOS) and increases in immunohistochemical staining for iNOS and nitrotyrosine were observed. However, the level of nitrite + nitrate was unchanged. An increase in enzymatic activity, upregulation of mRNA expression, and immunostaining for arginase I was detected in the lung tissue and serum. Moreover, increases in both arginase I and II were revealed by immunoblotting. Goblet cell hyperplasia in bronchial epithelial cells and increasing collagen synthesis around the bronchus were also observed. These results suggested that an increase in arginase may lead to decreased availability of arginine for nitric oxide synthase and may contribute to the remodeling of the lung.
Takemoto
Kei
K
Shin Nippon Biomedical Laboratories, Tokyo, Japan.
Ogino
Keiki
K
Shibamori
Masafumi
M
Gondo
Toshikazu
T
Hitomi
Yoshiaki
Y
Takigawa
Tomoko
T
Wang
Da-Hong
DH
Takaki
Jiro
J
Ichimura
Hiroshi
H
Fujikura
Yoshihisa
Y
Ishiyama
Hironobu
H
D000818:Animals; D001119:Arginase / Q000235:genetics*; D001249:Asthma / Q000201:enzymology* / Q000473:pathology*; D003094:Collagen / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D020397:Goblet Cells / Q000201:enzymology / Q000473:pathology; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D051379:Mice; D009566:Nitrates / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics*; D009573:Nitrites / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000096:biosynthesis; D015854:Up-Regulation / Q000235:genetics*
D016207:Cytokines; D007527:Isoenzymes; D009566:Nitrates; D009573:Nitrites; D012333:RNA, Messenger; C002744:3-nitrotyrosine; D014443:Tyrosine; D003094:Collagen; D052247:Nitric Oxide Synthase Type II; D001119:Arginase