title: Acetylated histone H3 and H4 mark the upregulated LMP2A promoter of Epstein-Barr virus in lymphoid cells.
abstract: We analyzed the levels of acetylated histones and histone H3 dimethylated on lysine 4 (H3K4me2) at the LMP2A promoter (LMP2Ap) of Epstein-Barr virus in well-characterized type I and type III lymphoid cell line pairs and additionally in the nasopharyngeal carcinoma cell line C666-1 by using chromatin immunoprecipitation. We found that enhanced levels of acetylated histones marked the upregulated LMP2Ap in lymphoid cells. In contrast, in C666-1 cells, the highly DNA-methylated, inactive LMP2Ap was also enriched in acetylated histones and H3K4me2. Our results suggest that the combinatorial effects of DNA methylation, histone acetylation, and H3K4me2 modulate the activity of LMP2Ap.
authors: Microbiological Research Group, National Center for Epidemiology, Pihenö u. 1, H-1529 Budapest, Hungary.
authors: Borbala
authors: B
authors: Gerle
authors: Anita
authors: A
authors: Koroknai
authors: György
authors: G
authors: Fejer
authors: Agnes
authors: A
authors: Bakos
authors: Ferenc
authors: F
authors: Banati
authors: Kalman
authors: K
authors: Szenthe
authors: Hans
authors: H
authors: Wolf
authors: Hans Helmut
authors: HH
authors: Niller
authors: Janos
authors: J
authors: Minarovits
authors: Daniel
authors: D
authors: Salamon
mesh_terms: D000107:Acetylation; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D004279:DNA, Viral / Q000737:chemistry*; D004854:Herpesvirus 4, Human / Q000737:chemistry / Q000502:physiology*; D006657:Histones / Q000032:analysis*; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry / Q000821:virology*; D008745:Methylation; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014763:Viral Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D006657:Histones; D014763:Viral Matrix Proteins