title: The chimeric cytokine Hyper-IL-6 enhances the efficiency of lentiviral gene transfer in hepatocytes both in vitro and in vivo.
abstract: Lentiviral vectors have been used for gene transfer into the liver but their ability to efficiently transduce quiescent hepatocytes remains controversial. Lentivirus-mediated gene transfer is more efficient in cycling cells. We determine the effect of H-IL6 in the lentiviral transduction. The lentiviral vector was used to transduce HepG2 cells and mice liver cells, previously treated with H-IL6. The highest transduction level was observed in HepG2 cells treated with 30 ng/mL H-IL6 and in the mice that received 4 microg H-IL6. Our results suggest that H-IL6 is an inducer of lentiviral gene transfer into the liver cells without any toxicity.
authors: Hemocentro de Ribeirão Preto, Centro de Terapia Celular - FMRP/USP, Monte Alegre, Ribeirão Preto, SP, Brazil.
authors: Virgínia
authors: V
authors: Picanço-Castro
authors: Aparecida Maria
authors: AM
authors: Fontes
authors: Stefan
authors: S
authors: Heinz
authors: Torsten
authors: T
authors: Tonn
authors: Dimas Tadeu
authors: DT
authors: Covas
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006678:HIV / Q000235:genetics*; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D015850:Interleukin-6; D011993:Recombinant Fusion Proteins