Combinatorial enzymatic synthesis of heparan sulfate.
Escherichia coli K5 heparosan was enzymatically modified by Chen and colleagues to construct a library of heparan sulfate polysaccharides for evaluation, leading to the discovery that a 2-O-sulfoiduronic acid residue is not essential for antithrombin-mediated anticoagulant activity in larger oligosaccharide and polysaccharide structures.
Linhardt
Robert J
RJ
Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA. linhar@rpi.edu
Kim
Jin-Hwan
JH
D000990:Antithrombin III / Q000378:metabolism; D002240:Carbohydrate Sequence; D020650:Combinatorial Chemistry Techniques; D004798:Enzymes / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D006497:Heparan Sulfate / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism
D000990:Antithrombin III; D004798:Enzymes; D006497:Heparan Sulfate