Ultraviolet B irradiation affects resistance of rainbow trout (Oncorhynchus mykiss) against bacterium Yersinia ruckeri and trematode Diplostomum spathaceum.
Ultraviolet B (UVB) radiation is known to have various effects on the immune system of fish, but the effect on the actual disease resistance has remained largely unknown. Here we studied the effect of UVB on the resistance of rainbow trout (Oncorhynchus mykiss) against a bacterium Yersinia ruckeri, the causative agent of enteric red mouth disease, and a trematode parasite Diplostomum spathaceum, which causes cataracts in fish. The fish were exposed to UVB irradiation seven times in 14 days, and inoculated intraperitoneally with Y. ruckeri on day 5 after the first irradiation. On day 2 postinfection (p.i.), the number of viable bacteria in the kidney was lower in UVB-exposed than in unexposed fish. However, on day 8 p.i., UVB-irradiated fish had not been able to clear remaining Y. ruckeri effectively, and had a slightly higher bacterial load than controls. A similar, although not significant, effect was seen in the bacterial numbers in spleen. In the other experiment, fish were exposed to UVB for six consecutive days and then exposed to D. spathaceum. A significantly higher number of parasites was detected in the eyes of irradiated fish, indicating reduced resistance against the pathogen. Furthermore, UVB-irradiation altered the immunological and hematological parameters of fish, which also verified the immunomodulatory potential of UVB in the present study.
Markkula
S Eveliina
SE
Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, Finland. emarkkul@cc.jyu.fi
Karvonen
Anssi
A
Salo
Harri
H
Tellervo Valtonen
E
E
Ilmari Jokinen
E
E
D000818:Animals; D015169:Colony Count, Microbial; D017686:Oncorhynchus mykiss / Q000382:microbiology*; D014200:Trematoda / Q000472:pathogenicity*; D014466:Ultraviolet Rays; D044124:Yersinia ruckeri / Q000472:pathogenicity*