Flow-injection determination of acetone with diazotized anthranilic acid through a fluorescent reaction intermediate.
Acetone and diazotized anthranilic acid react in alkaline solution, giving a fluorescent intermediate that can be measured at excitation and emission wavelengths of 305 and 395 nm, respectively. Based on this, a fluorimetric flow-injection method is proposed for the determination of acetone in aqueous solution. Under the proposed conditions, acetone can be detected at concentrations higher than 8 x 10(-7)M, with a linear application range from 1 x 10(-6) to 2 x 10(-4)M and an R.S.D. of 2.7% (1.0 x 10(-5)M, n=10). A sampling frequency of 24h(-1) is achieved. Some potentially interfering species are investigated.
García de María
C
C
Dpto. de Química Analítica, Nutrición y Bromatología, University of Salamanca, Plaza de la Merced s/n, E-37008 Salamanca, Spain.
Hueso Domínguez
K B
KB
Martín Garrido
N
N
D000096:Acetone / Q000032:analysis* / Q000737:chemistry*; D000468:Alkalies; D001391:Azo Compounds / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D012972:Sodium Hydroxide; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D062367:ortho-Aminobenzoates / Q000737:chemistry*
D000468:Alkalies; D001391:Azo Compounds; D012996:Solutions; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; D000096:Acetone; D012972:Sodium Hydroxide