title: Physical plasticity of the nucleus in stem cell differentiation.
abstract: Cell differentiation in embryogenesis involves extensive changes in gene expression structural reorganization within the nucleus, including chromatin condensation and nucleoprotein immobilization. We hypothesized that nuclei in naive stem cells would therefore prove to be physically plastic and also more pliable than nuclei in differentiated cells. Micromanipulation methods indeed show that nuclei in human embryonic stem cells are highly deformable and stiffen 6-fold through terminal differentiation, and that nuclei in human adult stem cells possess an intermediate stiffness and deform irreversibly. Because the nucleo-skeletal component Lamin A/C is not expressed in either type of stem cell, we knocked down Lamin A/C in human epithelial cells and measured a deformability similar to that of adult hematopoietic stem cells. Rheologically, lamin-deficient states prove to be the most fluid-like, especially within the first approximately 10 sec of deformation. Nuclear distortions that persist longer than this are irreversible, and fluorescence-imaged microdeformation with photobleaching confirms that chromatin indeed flows, distends, and reorganizes while the lamina stretches. The rheological character of the nucleus is thus set largely by nucleoplasm/chromatin, whereas the extent of deformation is modulated by the lamina.
authors: Molecular and Cell Biophysics Laboratory, 129 Towne Building, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors: J David
authors: JD
authors: Pajerowski
authors: Kris Noel
authors: KN
authors: Dahl
authors: Franklin L
authors: FL
authors: Zhong
authors: Paul J
authors: PJ
authors: Sammak
authors: Dennis E
authors: DE
authors: Discher
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology; D002413:Cations, Divalent / Q000494:pharmacology; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure*; D047108:Embryonic Development; D053595:Embryonic Stem Cells / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans
chemical_list: D002413:Cations, Divalent; D049452:Green Fluorescent Proteins