Papillomavirus binding factor binds to SAP30 and represses transcription via recruitment of the HDAC1 co-repressor complex.
Papillomavirus binding factor, PBF, identical to the Huntington's disease binding protein 2, HDBP2, is a nuclear-cytoplasmic shuttling factor with the ability to inhibit cell growth. It has been identified by its ability to bind to GC-rich sequence elements within upstream promoter regions of certain human papillomavirus (HPV) types and of the Huntingtin protein, respectively. Here, we show that PBF acts as a repressor of HPV transcription. This repression requires the DNA-binding activity of PBF, which we mapped to two C-terminal four-amino acids motifs conserved to the so-called e-tail of certain T-cell factors. Moreover, we show that PBF directly binds to SAP30 (Sin3-associated polypeptide of 30kDa) a component of the mSIN3A-HDAC1 complex, via amino acids 263-312. The addition of Trichostatin A, an inhibitor of HDACs, alleviated PBF-mediated repression. Thus, PBF-mediated repression of transcription involves specific DNA-binding and the recruitment of the SIN3A-HDAC1 complex.
Sichtig
Nadine
N
Institute of Virology, University of Cologne, Fürst-Pückler-Strasse 56, 50935 Cologne, Germany.
Körfer
Nadine
N
Steger
Gertrud
G
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000502:physiology*; D006367:HeLa Cells; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases / Q000378:metabolism* / Q000502:physiology*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D008969:Molecular Sequence Data; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000502:physiology*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D006877:Hydroxamic Acids; C113294:SAP30 protein, human; D014157:Transcription Factors; C484332:ZNF395 protein, human; C012589:trichostatin A; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases