Multimeric analysis in diagnosis of vWD variants in Indians.
von Willebrand disease is a common inherited bleeding disorder and the problem is undefined in developing countries due to limitation of its diagnostic facilities. The aim of the study was to diagnose vWD in patients with history of muco - cutaneous bleeding and characterization into its variants by multimeric analysis. 224 patients presenting with history of muco - cutaneous bleeding were selected. In all patients, platelet count, BT, PT, APTT, PF3 availability, clot solubility and factor VIII assay were done. Diagnosis of vWD was confirmed by RIPA, vWF: Ag, and vWF: RCo and its sub-characterization was done by multimeric analysis. 64 patients were diagnosed to have vWD. Of these, 21.9% were of type 1 vWD, 43.7% type 2 vWD, 1.6% acquired vWD and 32.8% type 3 vWD. By multimeric analysis, 2 patients had supranormal HMW multimers and two patients had normal distribution of vWF multimers were diagnosed as type 2M 'Vicenza'; and type 2M vWD respectively. It is concluded, that vWD is not an uncommon condition amongst Indian population.
Gupta
P K
PK
Department of Transfusion Medicine, Armed Forces Medical College, Pune. paw_5671@hotmail.com
Kumar
Harsh
H
Saxena
Renu
R
D000293:Adolescent; D000328:Adult; D001760:Bleeding Time / Q000379:methods; D002648:Child; D002675:Child, Preschool; D005169:Factor VIII / Q000032:analysis; D005260:Female; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D008297:Male; D008875:Middle Aged; D010314:Partial Thromboplastin Time / Q000379:methods; D010974:Platelet Aggregation; D010977:Platelet Factor 3 / Q000032:analysis; D014842:von Willebrand Diseases / Q000145:classification* / Q000175:diagnosis* / Q000453:epidemiology; D014841:von Willebrand Factor / Q000032:analysis
D014841:von Willebrand Factor; D010977:Platelet Factor 3; D005169:Factor VIII