title: Composition, enantiomeric distribution, and antimicrobial activity of Tanacetum argenteum subsp. flabellifolium essential oil.
abstract: Tanacetum argenteum (Lam.) Willd. subsp. flabellifolium (Boiss. & Heldr.) Grierson of Asteraceae is an endemic species in Turkey. Hydrodistillation of aerial parts using a Clevenger apparatus yielded an essential oil, which was subsequently analyzed by gas chromatography-mass spectroscopy (GC-MS). alpha-Pinene (29%), (E)-sesquilavandulol (16%), and camphor (14%) were found as main constituents. Enantiomeric distribution of the monoterpenes alpha-pinene and camphor was determined on a fused silica Lipodex E capillary column using a multidimensional gas chromatography-mass spectroscopy (MDGC-MS) system, (-)-alpha-pinene (86%), (+)-alpha-pinene (14%), and (-)-camphor (100%) enantiomeric distributions were found in the oil. Furthermore, antimicrobial activity of the oil was carried out using a micro-dilution assay against human pathogenic bacteria and the yeast Candida albicans resulting in moderate inhibitory concentrations (MIC=125 microg/mL).
authors: USDA-ARS-NPURU, The University of Mississippi, University, MS 38677, USA. ntabanca@olemiss.edu
authors: Nurhayat
authors: N
authors: Tabanca
authors: Fatih
authors: F
authors: Demirci
authors: Betül
authors: B
authors: Demirci
authors: David E
authors: DE
authors: Wedge
authors: K Hüsnü Can
authors: KH
authors: Baser
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000081008:Bicyclic Monoterpenes; D002164:Camphor / Q000737:chemistry; D002176:Candida albicans / Q000187:drug effects; D002701:Chloramphenicol / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D007654:Ketoconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D039821:Monoterpenes / Q000737:chemistry; D009822:Oils, Volatile / Q000032:analysis* / Q000494:pharmacology*; D012015:Reference Standards; D013237:Stereoisomerism; D031203:Tanacetum / Q000737:chemistry* / Q000145:classification
chemical_list: D000900:Anti-Bacterial Agents; D000081008:Bicyclic Monoterpenes; D039821:Monoterpenes; D009822:Oils, Volatile; D002701:Chloramphenicol; D002164:Camphor; C005451:alpha-pinene; D007654:Ketoconazole