title: Extracellular amino acid levels in the interpositus nucleus during classical eyeblink conditioning in alert cats.
abstract: The extracellular levels of selected amino acids in the cerebellar posterior interpositus nucleus (PIN) during classical eyeblink conditioning was analyzed in alert cats using a delay paradigm. Animals were prepared for the chronic recording of eyelid movements (with the magnetic search-coil technique) and the electromyographic activity of the orbicularis oculi muscle. With the help of a guide and push-pull cannulae, selected PIN sites were perfused daily during classical eyeblink conditioning. The perfusate was sampled at intervals of 5 min and analyzed with a high-pressure liquid chromatography- electrochemical detection (HPLC-EC) method. The analysis of push-pull perfusate revealed a significant increase in the release of glycine, taurine, and glutamate across the successive conditioning sessions, in parallel with the acquisition of eyelid conditioned responses (CRs). Both CRs and extracellular levels of these three amino acids returned to control values during extinction. Other amino acids (alanine, GABA, glutamine, serine, and threonine) did not undergo modifications in their extracellular concentrations across the training. Results are discussed with regard to the role of PIN in this type of associative learning.
authors: Department of Anatomy and Developmental Biology, University College London, London, United Kingdom.
authors: Lydia
authors: L
authors: Jiménez-Díaz
authors: Agnès
authors: A
authors: Gruart
authors: Francisco Javier
authors: FJ
authors: Miñano
authors: José María
authors: JM
authors: Delgado-García
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002415:Cats; D002529:Cerebellar Nuclei / Q000098:blood supply / Q000378:metabolism*; D002560:Cerebrovascular Circulation / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D003215:Conditioning, Eyelid / Q000502:physiology*; D004563:Electrochemistry; D004576:Electromyography; D005108:Extinction, Psychological / Q000502:physiology; D005110:Extracellular Space / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D008297:Male; D009801:Oculomotor Muscles / Q000502:physiology; D013654:Taurine / Q000378:metabolism
chemical_list: D000596:Amino Acids; D013654:Taurine; D018698:Glutamic Acid; D005998:Glycine