title: Commercial ampholytes used for isoelectric focusing may interfere with bioactivity based purification of antimicrobial peptides.
abstract: BioRad's Rotofor system has been frequently used for the purification of proteins and smaller peptides such as bacteriocins. In this study, we report that some commercially available ampholytes used with the Rotofor isoelectric focusing system possess antimicrobial activity, which may interfere with the purification of bacteriocins and bacteriocin-like substances.
authors: Department of Food Science, Rutgers, the State University of New Jersey, New Brunswick, New Jersey 08901, USA.
authors: Shadi
authors: S
authors: Riazi
authors: Sara
authors: S
authors: Dover
authors: Yevgeniy
authors: Y
authors: Turovskiy
authors: Michael L
authors: ML
authors: Chikindas
mesh_terms: D000665:Ampholyte Mixtures / Q000737:chemistry; D023181:Antimicrobial Cationic Peptides / Q000302:isolation & purification* / Q000378:metabolism; D001709:Biotechnology / Q000379:methods* / Q000592:standards; D007525:Isoelectric Focusing / Q000379:methods*
chemical_list: D000665:Ampholyte Mixtures; D023181:Antimicrobial Cationic Peptides