Evaluation of the diagnostic potential of region of deletion-1-encoded antigen culture filtrate protein-10 in pulmonary tuberculosis.
The ability of region of deletion-1 (RD-1)-encoded culture filtrate protein-10 (CFP-10) to supplement the sensitivity of 38-kDa antigen was studied using enzyme-linked immunosorbent assay in pulmonary tuberculosis (TB) patients and controls. The sensitivities for individual antigens ranged from 50% to 60%, and the specificity was 100% for immunoglobulin (Ig) G alone. When IgA results were added to IgG, the sensitivity increased. On combination of the isotype results, sensitivity increased to 61.8% and 57.2% (for 38-kDa antigen and CFP-10), respectively, and specificity changed to 98.8% and 99.4% for 38-kDa and CFP-10, respectively. Combination of results of both the antigens gave 82.4% sensitivity in smear-positive and culture-positive group, and 98.1% specificity. In smear-negative and culture-positive group, the sensitivity was 66.7%. In smear-negative and culture-negative cases, a sensitivity of 65.6% was obtained. This study demonstrates that the use of RD-1-encoded CFP-10 enhances the sensitivity of 38-kDa antigen and can be a useful diagnostic marker in TB.
Murthy
Madhan Kumar
MK
Department of Immunology, Tuberculosis Research Centre (ICMR), Chetput, Chennai, 600 031, India.
Parasa
Ramana Rao V
RR
Deenadayalan
Anbarasu
A
Sharma
Pawan
P
Raja
Alamelu
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000175:diagnosis*
C075966:Ag38 antigen, Mycobacterium tuberculosis; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C117940:CFP-10 protein, Mycobacterium tuberculosis; D008074:Lipoproteins