title: Confirmation of cannabinoids in meconium using two-dimensional gas chromatography with mass spectrometry detection.
abstract: Meconium has become the specimen of choice for determining fetal exposure to drugs of abuse, but its physical complexity can cause interferences from matrix effects. A new method to determine 9-carboxy-11-nor-Delta(9)-THC (9-THCA) and 11-hydroxy-Delta(9)-THC (11-OH-THC) using two-dimensional (2D) GC-MS was developed to reduce interferences and carryover. The method was validated using 70 spiked samples prepared in drug-free meconium and 46 residual patient specimens that were confirmed to contain cannabinoids. Ten patient specimens that failed to confirm due to interferences using the previous GC-MS method were analyzed using the new 2D method and 9-THCA was quantitated in all ten samples. The 2D GC-MS method improved chromatography which significantly reduced interferences and carryover when compared to the previous GC-MS method.
authors: ARUP Institute for Clinical and Experimental Pathology, ARUP Laboratories, Inc., 500 Chipeta Way, Salt Lake City, UT 84108-1221, USA. stephanie.marin@aruplab.com
authors: Stephanie J
authors: SJ
authors: Marin
authors: Rebecka
authors: R
authors: Coles
authors: Francis M
authors: FM
authors: Urry
authors: Gwendolyn A
authors: GA
authors: McMillin
mesh_terms: D002186:Cannabinoids / Q000032:analysis*; D013759:Dronabinol / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D008470:Meconium / Q000737:chemistry*; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D002186:Cannabinoids; D013759:Dronabinol