Successful embryo transfer in Tianzhu white yak using standard protocol.
The present study was carried out to investigate the efficiency of superovulation, oestrus synchronization, and embryo recovery in Tianzhu white yaks and also to confirm the pregnancy rate of black yaks, to which embryos collected from white yaks were transplanted. Forty-seven yaks were selected from different experiment groups, including 10 Tianzhu white female yaks (donor, group A) and 37 black female yaks (recipient, groups B and C). Superovulation of the donor was induced by the application procedure of CIDR-B+FSH+PG. Oestrus synchronization of recipients was induced using two methods: group B was given the same treatment as group A, except that the follicle-stimulating hormone (FSH) injection was not administered, whereas group C was injected with cloprostenol only once when corpus luteum (corpora lutea) was (were) palpated. The results showed that the oestrous rates in group A were higher (80%) than those in group B (60%) and group C (44.5%). As for the efficiency of superovulation, it was indicated that the mean numbers (+/-SD) of total corpora lutea, follicles, viable (transferable), and degenerated embryos were 4.75+/-2.19, 1.13+/-0.83, 2.50+/-1.31, and 1.38+/-0.92, respectively. The mean embryo recovery rates were 55.6%. All together, 18 viable embryos of Tianzhu white yak were obtained and 12 of them were transplanted to 10 recipients. The pregnancy rate was 50% and the delivery rate was 40%.
Yu
SiJiu
S
Faculty of Veterinary Medicine, Gansu Agriculture University, Lanzhou 730070, China. siyu@163.com
Ju
XiangHong
X
Wang
LiBin
L
Fan
JiangFeng
J
D000818:Animals; D002417:Cattle; D004624:Embryo Transfer / Q000662:veterinary*; D004973:Estrus Synchronization; D005260:Female; D005300:Fertility Agents, Female / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D011270:Pregnancy, Animal; D013480:Superovulation
D005300:Fertility Agents, Female; D007987:Gonadotropin-Releasing Hormone; D005640:Follicle Stimulating Hormone