title: (-)-Epigallocatechin-3-gallate interferes with mast cell adhesiveness, migration and its potential to recruit monocytes.
abstract: Mast cells are multipotent effector cells of the immune system. They are able to induce and enhance angiogenesis via multiple pathways. (-)-Epigallocatechin-3-gallate (EGCG), a major component of green tea and a putative chemopreventive agent, was reported to inhibit tumor invasion and angiogenesis, processes that are essential for tumor growth and metastasis. Using the human mast cell line HMC-1 and commercial cDNA macroarrays, we evaluated the effect of EGCG on the expression of angiogenesis-related genes. Our data show that among other effects, EGCG treatment reduces expression of two integrins (alpha5 and beta3) and a chemokine (MCP1), resulting in a lower adhesion of mast cells associated with a decreased potential to produce signals eliciting monocyte recruitment. These effects on gene expression levels are functionally validated by showing inhibitory effects in adhesion, aggregation, migration and recruitment assays.
authors: Procel Lab, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, and CIBERER, 29071, Málaga, Spain.
authors: E
authors: E
authors: Melgarejo
authors: M A
authors: MA
authors: Medina
authors: F
authors: F
authors: Sánchez-Jiménez
authors: L M
authors: LM
authors: Botana
authors: M
authors: M
authors: Domínguez
authors: L
authors: L
authors: Escribano
authors: A
authors: A
authors: Orfao
authors: J L
authors: JL
authors: Urdiales
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D016023:Integrins / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology*; D009000:Monocytes / Q000166:cytology*; D018919:Neovascularization, Physiologic / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D016023:Integrins; D002392:Catechin; C045651:epigallocatechin gallate