[Effects of nutrient concentration on the thickness of unstirred layers in the vicinity of small intestine mucose].
It has been found that the thickness of the unstirred layer of liquid, determined by glycine, increases from 247 +/- 12 to 319 +/- 14 mkm (p < 0.001) as the concentration of glycine increases from 5 to 40 mM; in the case of determination by glucose, it increases from 316 +/- 13 to 380 +/- 26 mkm, as the concentration of glucose increases from 10 to 40 mM, due to the fact that nutrients at higher concentrations penetrate to a greater depth to the basis of villi. The occurrence of the positive weak correlation between the layer thickness, determined by glucose, maltose, saccharose, alanine, leucine, and glial in rats and the parameter characterizing the rate of absorption (the maximum response of the short-circuit current to nutrient) contradicts the assumption that the transporters are distributed uniformly along the height of villus, and nutrients do not arrive at transporters located between the villi at a greater depth. Probably, the transporters are unevenly distributed along the height of villi. The method proposed may serve as a tool for studying the topography of distribution of the corresponding transporters for nutrients throughout the villus surface.
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D000818:Animals; D001693:Biological Transport, Active / Q000502:physiology; D022081:Cell Surface Extensions / Q000502:physiology; D005502:Food; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000502:physiology*; D007421:Intestine, Small / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar