[Fluid shear stress increases the Ca2+ concentration in bone-marrow derived osteoclast-like cells].
OBJECTIVE
To study the change of Ca2+ density in cultured osteoclast-like cells in response to fluid shear stress.


METHODS
Laser scanning confocal microscope and fluorescent probe were used to detect the free Ca2+ in osteoclast-like cells before and after undergoing fluid shear stress. The images were analyzed and compared with image software.


RESULTS
At 37 degrees C the free Ca2+ in osteoclast-like cells could be labelled effectively with 10 micromol/L Fluo-3/AM. Compared with contol group, the average intensity of Ca2+ fluorescent signal in osteoclast-like cells undergoing fluid shear stress increased significantly.


CONCLUSIONS
The Cal2+ concentration in bone-marrow derived osteoclast-like cells is sensitive to fluid shear stress, which suggests osteoclast-like cells modulate their function in response to fluid shear stress through the change of free Ca2+ concentration.
Chen
Ming
M
Dept. of Prosthodontics, Beijing Stomatologicul Hospital, Capital Medical University, Beijing 100050, China.
Liang
Xing
X
Wen
Ying
Y
Bai
Bao-jing
BJ
Huang
Meng-lu
ML
Gao
Wei-min
WM
D000814:Aniline Compounds; D001853:Bone Marrow; D001854:Bone Marrow Cells; D002118:Calcium; D002478:Cells, Cultured; D010010:Osteoclasts; D013314:Stress, Mechanical; D014966:Xanthenes
D000814:Aniline Compounds; D014966:Xanthenes; C059715:Fluo-3; D002118:Calcium