title: Transient receptor potential channel M5 and phospholipaseC-beta2 colocalizing in zebrafish taste receptor cells.
abstract: In mammals, transient receptor potential (TRP) channel M5 (TRPM5) is coexpressed with phospholipaseC-beta2 (PLC-beta2) in the taste receptor cells, and both PLC-beta2 and TRPM5 are essential elements in the signal transduction of sweet, bitter and umami stimuli. In this study, we identified the zebrafish homologue of TRPM5 (zfTRPM5) and examined its expression in the gustatory system by in-situ hybridization. Using a transgenic zebrafish line that expressed green fluorescent protein under the control of the PLC-beta2 promoter, we showed that zfTRPM5 is expressed in green fluorescent protein-labeled cells of the taste buds. These results demonstrate that zfTRPM5 and PLC-beta2 colocalize in zebrafish taste receptor cells, suggesting their crucial roles in taste signaling via the fish taste receptors.
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Yuki
authors: Y
authors: Yoshida
authors: Kana
authors: K
authors: Saitoh
authors: Yoshiko
authors: Y
authors: Aihara
authors: Shinji
authors: S
authors: Okada
authors: Takumi
authors: T
authors: Misaka
authors: Keiko
authors: K
authors: Abe
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002628:Chemoreceptor Cells / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D030541:Databases, Genetic; D049452:Green Fluorescent Proteins / Q000235:genetics; D017403:In Situ Hybridization; D054799:Phospholipase C beta / Q000235:genetics / Q000378:metabolism*; D011956:Receptors, Cell Surface; D050053:TRPM Cation Channels / Q000235:genetics / Q000378:metabolism*; D013650:Taste Buds / Q000378:metabolism*; D015027:Zebrafish / Q000502:physiology*
chemical_list: D011956:Receptors, Cell Surface; D050053:TRPM Cation Channels; D049452:Green Fluorescent Proteins; D004247:DNA; D054799:Phospholipase C beta