title: A combination of intravenous and dietary docosahexaenoic acid significantly improves outcome after spinal cord injury.
abstract: Previous studies have shown that omega-3 polyunsaturated fatty acids such as alpha-linolenic acid and docosahexaenoic acid (DHA) are neuroprotective in models of spinal cord injury (SCI) in rodents. However, the mechanism of action underlying these effects has not been elucidated, and the optimum treatment regime remains to be defined. We have therefore carried out a detailed analysis of the effects of DHA in adult rats subject to thoracic compression SCI. Saline or DHA (250 nmol/kg) was administered intravenously (i.v.) 30 min after compression. After injury, the saline group received a standard control diet for 1 or 6 weeks, whereas DHA-injected animals received either a control or a DHA-enriched diet (400 mg/kg/day) for 1 or 6 weeks. Other groups received a DHA-enriched diet only for 1 week following injury, or received acute DHA (250 nmol/kg; i.v.) treatment delayed up to 3 h after injury. We also assessed oxidative stress and the inflammatory reaction at the injury site, neuronal and oligodendrocyte survival and axonal damage and the locomotor recovery. At 24 h, lipid peroxidation, protein oxidation, RNA/DNA oxidation and the induction of cyclooxygenase-2 were all significantly reduced by i.v. DHA administration. At 1 week and 6 weeks, macrophage recruitment was reduced and neuronal and oligodendrocyte survival was substantially increased. Axonal injury was reduced at 6 weeks. Locomotor recovery was improved from day 4, and sustained up to 6 weeks. Rats treated with a DHA-enriched diet in addition to the acute DHA injection were not significantly different from the acute DHA-treated animals at 1 week, but at 6 weeks showed additional improvements in both functional and histological outcomes. DHA treatment was ineffective if the acute injection was delayed until 3 h post-injury, or if the DHA was administered for 1 week solely by diet. Our results in a clinically relevant model of SCI show that significant neuroprotection can be obtained by combining an initial acute i.v. injection of DHA with a sustained dietary supplementation. Given that the safety and tolerability of preparations enriched in omega-3 fatty acids is already well-documented, such a combined DHA treatment regime deserves consideration as a very promising approach to SCI management.
authors: Neuroscience Centre, Institute of Cell & Molecular Science, Queen Mary University of London, UK. w.huang@qmul.ac.uk
authors: W L
authors: WL
authors: Huang
authors: V R
authors: VR
authors: King
authors: O E
authors: OE
authors: Curran
authors: S C
authors: SC
authors: Dyall
authors: R E
authors: RE
authors: Ward
authors: N
authors: N
authors: Lal
authors: J V
authors: JV
authors: Priestley
authors: A T
authors: AT
authors: Michael-Titus
mesh_terms: D000818:Animals; D001369:Axons / Q000473:pathology; D002470:Cell Survival; D003131:Combined Modality Therapy; D051546:Cyclooxygenase 2 / Q000032:analysis; D019587:Dietary Supplements; D004281:Docosahexaenoic Acids / Q000008:administration & dosage* / Q000627:therapeutic use; D005260:Female; D007150:Immunohistochemistry; D007275:Injections, Intravenous; D015227:Lipid Peroxidation; D023421:Models, Animal; D009474:Neurons / Q000473:pathology; D018696:Neuroprotective Agents / Q000008:administration & dosage* / Q000627:therapeutic use; D009836:Oligodendroglia / Q000473:pathology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D013117:Spinal Cord Compression; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D018696:Neuroprotective Agents; D004281:Docosahexaenoic Acids; D051546:Cyclooxygenase 2