[Effect of cyclic-tension force on the expression of osteogenesis genes in human periodontal ligament cells].
OBJECTIVE
To investigate the effect of cyclic-tension force on the expression of osteogenesis genes in human periodontal ligament cells (HPDLC).


METHODS
HPDLC were cultured on flexible-bottomed plates and subjected to 12% elongation by strain unit at 6 cycles/min (i.e.5-s elongation and 5-s relaxation) for 48 hours in the experimental groups. GEArray Q series Human Osteogenesis Gene Array was used to identify the genes expressed in HPDLC, including growth factors and associated molecules, extracellular matrix and its associated proteins, cell adhesion molecules and housekeeping genes. The changes in the expression of 96 representative transcripts were determined by arrayed cDNA hybridization.


RESULTS
After application of tension force, 21 genes were significantly upregulated, including 10 growth factors and associated molecules, 10 extracellular matrix and its associated protein and 1 cell adhesion molecules. Two genes were significantly downregulated, including 1 growth factors and associated molecules and 1 cell adhesion molecules.


CONCLUSIONS
The HPDLC can differentiate into osteoblast-like cells by mechanical stretch induction through the activation of some osteogenesis genes.
Li
Yong-Ming
YM
Department of Orthodontics, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China. liyongming@fmmu.edu.cn
Yang
Lei
L
Ding
Yin
Y
Li
Dong
D
D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D002648:Child; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D051957:MSX1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology* / Q000378:metabolism; D013314:Stress, Mechanical
D015815:Cell Adhesion Molecules; D051957:MSX1 Transcription Factor; C484083:MSX1 protein, human; C512478:EGFR protein, human; D066246:ErbB Receptors