title: Surfactant-enhanced anaerobic acidogenesis of Canna indica L. by rumen cultures.
abstract: Polyoxyethylene sorbitan monoolate (Tween 80) was used to enhance the anaerobic acidogenesis of Canna indica L. (canna) by rumen culture in this study. Dose of Tween 80 at 1 ml/l enhanced the volatile fatty acids (VFA) production from the acidogenesis of canna compared to the control. However, Tween 80 at higher dosages than 5 ml/l inhibited the rumen microbial activity and reduced the VFA yield. Response surface methodology was successfully used to optimize the VFA yield. A maximum of VFA yield of 0.147 g/g total solids (TS) added was obtained at canna and Tween 80 concentrations of 6.3g TS/l and 2.0 ml/l, respectively. Dosage of Tween 80 at 1-3.75 ml/l reduced the unproductive adsorption of microbes or enzymes on the lignin part in canna and increased microbial activity. A high VFA production was achieved from canna presoaked with Tween 80, suggesting that the structure of canna was disrupted by Tween 80.
authors: Laboratory of Environmental Biotechnology, School of Chemistry, University of Science and Technology of China, Hefei 230026, China.
authors: Zheng-Bo
authors: ZB
authors: Yue
authors: Han-Qing
authors: HQ
authors: Yu
authors: Zhen-Hu
authors: ZH
authors: Hu
authors: Hideki
authors: H
authors: Harada
authors: Yu-You
authors: YY
authors: Li
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000693:Anaerobiosis / Q000187:drug effects; D000818:Animals; D001673:Biodegradation, Environmental / Q000187:drug effects; D027581:Cannabaceae / Q000187:drug effects* / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000032:analysis; D011136:Polysorbates / Q000494:pharmacology*; D012044:Regression Analysis; D012417:Rumen / Q000187:drug effects* / Q000201:enzymology / Q000382:microbiology*; D013379:Substrate Specificity / Q000187:drug effects; D013501:Surface-Active Agents / Q000494:pharmacology*
chemical_list: D005232:Fatty Acids, Volatile; D011136:Polysorbates; D013501:Surface-Active Agents; D002480:Cellulase; C019826:carboxymethylcellulase