A comparative proteinomic analysis of nipple aspiration fluid from healthy women and women with breast cancer.
This pilot study examines the feasibility of nipple aspiration to distinguish women with breast cancer from healthy women using surface-enhanced laser desorption ionisation time-of-flight mass spectrometry (SELDI-TOF/MS). Nipple aspiration fluid (NAF) was collected from each breast in 21 women newly diagnosed with unilateral breast cancer and 44 healthy women. No differences were found when proteomic profiles of NAF from the cancer-bearing breast and the contralateral non-cancerous breast were compared. In contrast, 9 protein peaks were significantly different between the cancer-bearing breast compared with healthy women and 10 peaks were significantly different between the contralateral healthy breast and healthy women (P<0.05). These data suggest that invasive breast cancer may result in a field change across both breasts and that proteomic profiling of NAF may have more value in breast cancer risk assessment than as a diagnostic or screening tool.
Noble
J L
JL
Department of Academic Surgery (Breast Unit), The Royal Marsden NHS Foundation Trust, Fulham Road, London SW3 6JJ, UK.
Dua
R S
RS
Coulton
G R
GR
Isacke
C M
CM
Gui
G P H
GP
D000328:Adult; D044963:Biopsy, Fine-Needle / Q000379:methods; D001826:Body Fluids / Q000737:chemistry / Q000166:cytology; D001943:Breast Neoplasms / Q000737:chemistry / Q000175:diagnosis*; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*; D009558:Nipples / Q000378:metabolism; D010865:Pilot Projects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D016896:Treatment Outcome
D009363:Neoplasm Proteins