Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures.
To increase the yield of heterologous production of the class II bacteriocin DvnRV41 with Escherichia coli Origami (DE3) (pLysS/pCR03), induction of bacteriocin gene expression was optimized by varying the inducer isopropyl beta-D-thiogalactopyranoside (IPTG) concentration (0-2 mM), and controlled batch and fed-batch cultures were tested on a 2-L scale. A concentration of 0.5 mM IPTG was found to be optimal for cell growth and bacteriocin production. Shake flask cultivation of E. coli Origami (DE3) (pLysS/pCR03) gave biomass and bacteriocin yields of 1.54 +/- 0.06 g cdw/l and 18 +/- 1 mg DvnRV41/l, respectively. Biomass (2.70 +/- 0.06 and 6.8 +/- 0.6 g cdw/l, respectively) and bacteriocin yields (30 and 74 mg DvnRV41 per liter, respectively) were both increased with batch and fed-batch compared to shake flask cultures. Bacteriocin yields reported in this study are among the highest published for other heterologous expression systems in shake flasks.
Yildirim
Selcuk
S
Laboratory of Food Biotechnology, Institute of Food Science and Nutrition, ETH (Swiss Federal Institute of Technology) Zurich, LFV C20 Schmelzbergstrasse 7, CH-8092, Zurich, Switzerland.
Konrad
Daniel
D
Calvez
Ségolène
S
Drider
Djamel
D
Prévost
Hervé
H
Lacroix
Christophe
C
D001430:Bacteriocins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology; D007544:Isopropyl Thiogalactoside / Q000378:metabolism; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification
D001430:Bacteriocins; D003470:Culture Media; D011994:Recombinant Proteins; C116372:divercin V41; D007544:Isopropyl Thiogalactoside