Applications of nanobiotechnology in clinical diagnostics.
BACKGROUND
Nanobiotechnologies are being applied to molecular diagnostics and several technologies are in development.


METHODS
This review describes nanobiotechnologies that are already incorporated in molecular diagnostics or have potential applications in clinical diagnosis. Selected promising technologies from published literature as well as some technologies that are in commercial development but have not been reported are included.


RESULTS
Nanotechnologies enable diagnosis at the single-cell and molecule levels, and some can be incorporated in current molecular diagnostic methods, such as biochips. Nanoparticles, such as gold nanoparticles and quantum dots, are the most widely used, but various other nanotechnological devices for manipulation at the nanoscale as well as nanobiosensors are also promising for potential clinical applications.


CONCLUSIONS
Nanotechnologies will extend the limits of current molecular diagnostics and enable point-of-care diagnostics, integration of diagnostics with therapeutics, and development of personalized medicine. Although the potential diagnostic applications are unlimited, the most important current applications are foreseen in the areas of biomarker discovery, cancer diagnosis, and detection of infectious microorganisms. Safety studies are needed for in vivo use. Because of its close interrelationships with other technologies, nanobiotechnology in clinical diagnosis will play an important role in the development of nanomedicine in the future.
Jain
Kewal K
KK
Jain PharmaBiotech, Basel, Switzerland. jain@pharmabiotech.ch
D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods; D019411:Clinical Laboratory Techniques; D020732:Cytogenetic Analysis / Q000379:methods; D006801:Humans; D008280:Magnetics; D046228:Microarray Analysis / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D053758:Nanoparticles; D036103:Nanotechnology; D013234:Stem Cells / Q000166:cytology
D015415:Biomarkers