title: [Silencing HSV1 gD expression in cultured cells by RNA interference].
abstract: To explore the anti-HSV-1 effect of silencing gD gene expression by RNA interference, five 21-nucleotide duplex small interfering RNAs(siRNAs) targeting the HSV1 gD sequence were designed and the gD-EGFP fusion gene expression vector was constructed, then co-transfected into Vero cell, and screened the effective siRNA through analyzing the intensity of the EGFP fluorescence. Finally, the anti-HSV1 effect was confirmed by plaque reduction assay, real-time PCR and daughter virus titration of HSV1 infected Vero cells transfected with siRNAs. The study demonstrated that siRNAs could effectively and specifically inhibit gD gene expression in HSV1-infected cells, but only had a little effect on HSV1 infection, so taking gD as the target of siRNA against HSV1 needs further study.
authors: Biomedicine Research and Development Center of Jinan University, Guangzhou 510632, China.
authors: Qin-Chang
authors: QC
authors: Zhu
authors: Zhe
authors: Z
authors: Ren
authors: Chun-Long
authors: CL
authors: Zhang
authors: Mei-Ying
authors: MY
authors: Zhang
authors: Hong-Juan
authors: HJ
authors: Liao
authors: Qiu-Ying
authors: QY
authors: Liu
authors: Pei-Zhuo
authors: PZ
authors: Zhang
authors: Jiu-Xiang
authors: JX
authors: Li
authors: Chao-Feng
authors: CF
authors: Hu
authors: Hua-Dong
authors: HD
authors: Wang
authors: Yi-Fei
authors: YF
authors: Wang
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D049452:Green Fluorescent Proteins