Synthesis and biodegradation of nanogels as delivery carriers for carbohydrate drugs.
Biodegradable nanogels loaded with rhodamine B isothiocyanate-dextran (RITC-Dx) as a model for water-soluble biomacromolecular drugs were prepared using atom-transfer radical polymerization (ATRP) in a cyclohexane inverse miniemulsion in the presence of a disulfide-functionalized dimethacrylate cross linker. UV-vis spectroscopy was used to characterize the extent of incorporation of RITC-Dx into the nanogels. The loading efficiency of RITC-Dx into the nanogels exceeded 80%. These nanogels were degraded into polymeric sols in a reducing environment to release the encapsulated carbohydrate drugs. The released carbohydrate biomolecules specifically interacted with concanavalin A in water, suggesting that the biodegradable nanogels could be used as carriers to deliver carbohydrate drugs that can be released upon degradation to bind to pathogens based on lectins.
Oh
Jung Kwon
JK
Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA.
Siegwart
Daniel J
DJ
Matyjaszewski
Krzysztof
K
D002241:Carbohydrates / Q000008:administration & dosage*; D003485:Cyanates; D003911:Dextrans; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D011094:Polyethyleneimine / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D012235:Rhodamines; D013053:Spectrophotometry
D002241:Carbohydrates; D003485:Cyanates; D003911:Dextrans; D004337:Drug Carriers; D000080385:Nanogels; D012235:Rhodamines; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D011094:Polyethyleneimine