Osmotic regulation of cellular glucose uptake.
This chapter describes various approaches allowing the study of hyperosmolarity in the functions of 3T3-L1 adipocytes. Hyperosmolarity mimics insulin responses, such as glucose uptake and membrane ruffling, but also antagonizes these insulin effects, which can be evaluated in 3T3-L1 adipocytes. The molecular mechanisms of these effects can be also investigated by measuring the activation of different signaling pathways: (i) the phosphorylation of docking proteins on tyrosine and serine residues (serines 307 and 632), (ii) the phosphorylation of serine/threonine kinases, and (iii) the activation of phosphatidylinositol 3-kinase.
Gual
Philippe
P
INSERM U 568, University of Nice Sophia-Antipolis, Nice, France.
Gonzalez
Teresa
T
Gremeaux
Thierry
T
Le Marchand-Brustel
Yannick
Y
Tanti
Jean-François
JF
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D007329:Insulin Antagonists; D051379:Mice; D009997:Osmotic Pressure; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
D051275:Glucose Transporter Type 4; D007329:Insulin Antagonists; D019869:Phosphatidylinositol 3-Kinases; D005947:Glucose