title: Leishmania amazonensis infection impairs differentiation and function of human dendritic cells.
abstract: Dendritic cells (DCs) are of utmost importance in initiating an immune response and may also function as targets for pathogens. The presence of pathogens inside DCs is likely to impair their functions and thus, influence immune responses. In the present report, we evaluated the impact of the presence of Leishmania amazonensis during differentiation and maturation of human monocyte-derived DCs. The presence of live L. amazonensis parasites during DC differentiation led to a significant decrease in CD80 (92%) and CD1a (56%) expression and an increase in CD86 (56%) cell surface expression. Phenotypic changes were accompanied by a lower secretion of IL-6, observed after 6 days of DC differentiation in the presence of L. amazonensis. DCs differentiated in the presence of L. amazonensis were used as APC in an autologous coculture, and lower amounts of IFN-gamma were obtained compared with control DCs differentiated in the absence of parasites. The effect of heat-killed parasites, but not of Leishmania antigen, during DC differentiation and maturation was similar to that observed with viable parasites. During maturation, the presence of live L. amazonensis parasites, but not of soluble Leishmania antigen, led to a decrease in IL-6 and IL-10 production. In this way, we observed that the parasite is able to abrogate full DC differentiation, causing a delay in the immune response and likely, favoring its establishment in human hosts.
authors: Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil.
authors: Cecilia
authors: C
authors: Favali
authors: Natália
authors: N
authors: Tavares
authors: Jorge
authors: J
authors: Clarêncio
authors: Aldina
authors: A
authors: Barral
authors: Manoel
authors: M
authors: Barral-Netto
authors: Claudia
authors: C
authors: Brodskyn
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D002448:Cell Adhesion; D002454:Cell Differentiation; D002470:Cell Survival; D018920:Coculture Techniques; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000166:cytology* / Q000469:parasitology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007894:Leishmania mexicana / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D009000:Monocytes / Q000166:cytology / Q000469:parasitology; D010271:Parasites / Q000166:cytology / Q000276:immunology / Q000502:physiology; D011528:Protozoan Infections / Q000276:immunology*; D012995:Solubility
chemical_list: D000953:Antigens, Protozoan; D016207:Cytokines; D015850:Interleukin-6; D007371:Interferon-gamma