Antibodies induced by Plasmodium falciparum merozoite surface antigen-2-designed pseudopeptides possess neutralizing properties of the in vitro malarial infection.
Pseudopeptide chemistry is gaining ground in the field of synthetic vaccine development. We have previously demonstrated the potential scope of introducing reduced amide peptide bond isosters in a site-directed design for obtaining structurally modified probes able to induce malaria infection-neutralizing antibodies derived from the MSP-1 antigen. This work reports the functional properties of polyclonal and monoclonal antibodies induced by site-directed designed MSP-2 N-terminus pseudopeptides and their capacity for antibody isotype switching in in vitro immunization. Structural properties of the native peptide and its pseudopeptide analogs are discussed within the context of these novel pseudopeptides' induced monoclonal antibody functional and physical-chemical properties.
Lozano
José Manuel
JM
Fundación Instituto de Inmunología de Colombia, Colombia. jm_lozano@fidic.org.co
Montoya-Fajardo
Francy J
FJ
Hoebeke
Johan
J
Cifuentes
Gladys H
GH
Forero
Martha
M
Patarroyo
Manuel Elkin
ME
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004912:Erythrocytes / Q000469:parasitology; D000882:Haplorhini; D016778:Malaria, Falciparum / Q000276:immunology*; D020066:Merozoite Surface Protein 1 / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000276:immunology*; D010963:Plasmodium falciparum / Q000276:immunology*
D000911:Antibodies, Monoclonal; D020066:Merozoite Surface Protein 1; D010455:Peptides