Dried blood spots versus sera for detection of rubella virus-specific immunoglobulin M (IgM) and IgG in samples collected during a rubella outbreak in Peru.
Most persons with rubella virus-specific immunoglobulin M (IgM)- or IgG-positive sera tested positive (98% [n = 178] and 99% [n = 221], respectively) using paired filter paper dried blood spot (DBS) samples, provided that DBS indeterminate results were called positive. For persons with IgM- or IgG-negative sera, 97% and 98%, respectively, were negative using DBS.
Helfand
Rita F
RF
Centers for Disease Control and Prevention, 1600 Clifton Rd., NE, Mailstop C-12, Atlanta, GA 30333, USA. rzh7@cdc.gov
Cabezas
Cesar
C
Abernathy
Emily
E
Castillo-Solorzano
Carlos
C
Ortiz
Ana Cecilia
AC
Sun
Hong
H
Osores
Fernando
F
Oliveira
Lucia
L
Whittembury
Alvaro
A
Charles
Myrna
M
Andrus
Jon
J
Icenogle
Joe
J
D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D001800:Blood Specimen Collection; D004196:Disease Outbreaks; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood* / Q000276:immunology; D010568:Peru / Q000453:epidemiology; D012409:Rubella / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology / Q000821:virology; D012412:Rubella virus / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C040901:rubella antibodies