title: Conformational change of erythroid alpha-spectrin at the tetramerization site upon binding beta-spectrin.
abstract: We previously determined the solution structures of the first 156 residues of human erythroid alpha-spectrin (SpalphaI-1-156, or simply Spalpha). Spalpha consists of the tetramerization site of alpha-spectrin and associates with a model beta-spectrin protein (Spbeta) with an affinity similar to that of native alpha- and beta-spectrin. Upon alphabeta-complex formation, our previous results indicate that there is an increase in helicity in the complex, suggesting conformational change in either Spalpha or Spbeta or in both. We have now used isothermal titration calorimetry, circular dichroism, static and dynamic light scattering, and solution NMR methods to investigate properties of the complex as well as the conformation of Spalpha in the complex. The results reveal a highly asymmetric complex, with a Perrin shape parameter of 1.23, which could correspond to a prolate ellipsoid with a major axis of about five and a minor axis of about one. We identified 12 residues, five prior to and seven following the partial domain helix in Spalpha that moved freely relative to the structural domain in the absence of Spbeta but when in the complex moved with a mobility similar to that of the structural domain. Thus, it appears that the association with Spbeta induced an unstructured-to-helical conformational transition in these residues to produce a rigid and asymmetric complex. Our findings may provide insight toward understanding different association affinities of alphabeta-spectrin at the tetramerization site for erythroid and non-erythroid spectrin and a possible mechanism to understand some of the clinical mutations, such as L49F of alpha-spectrin, which occur outside the functional partial domain region.
authors: Department of Chemistry, University of Illinois at Chicago 60607, USA.
authors: Fei
authors: F
authors: Long
authors: Dan
authors: D
authors: McElheny
authors: Shaokai
authors: S
authors: Jiang
authors: Sunghyouk
authors: S
authors: Park
authors: Michael S
authors: MS
authors: Caffrey
authors: Leslie W-M
authors: LW
authors: Fung
mesh_terms: D002151:Calorimetry / Q000379:methods; D002845:Chromatography / Q000379:methods; D002942:Circular Dichroism; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015233:Models, Statistical; D008970:Molecular Weight; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D012542:Scattering, Radiation; D013049:Spectrin / Q000737:chemistry*
chemical_list: D011994:Recombinant Proteins; D013049:Spectrin