title: The fragile X mental retardation protein is a molecular adaptor between the neurospecific KIF3C kinesin and dendritic RNA granules.
abstract: Fragile X mental retardation 1 protein (FMRP) is an RNA-binding protein whose absence results in the fragile X syndrome, the most common inherited form of mental retardation. FMRP contains multiple domains with apparently differential affinity to mRNA and interacts also with protein partners present in ribonucleoprotein complexes called RNA granules. In neurons, these particles travel along dendrites and axons to translocate mRNAs to specific destinations in spines and growth cones, where local synthesis of neuro-specific proteins is taking place. However, the molecular mechanisms of how RNA granules are translocated to dendrites remained unknown. We report here the identification and characterization of the motor protein KIF3C as a novel FMRP-interacting protein. In addition, using time-lapse videomicroscopy, we studied the dynamics and kinetics of FMRP-containing RNA granules in dendrites and show that a KIF3C dominant-negative impedes their distal transport. We therefore propose that, in addition to modulate the translation of its mRNA targets, FMRP acts also as a molecular adaptor between RNA granules and the neurospecific kinesin KIF3C that powers their transport along neuronal microtubules.
authors: Unité de Recherche en Génétique Humaine et Moléculaire, Centre de recherche Hôpital Saint-François d'Assise, le CHUQ, Québec, Canada G1L 3L5.
authors: Laetitia
authors: L
authors: Davidovic
authors: Xavier H
authors: XH
authors: Jaglin
authors: Aude-Marie
authors: AM
authors: Lepagnol-Bestel
authors: Sandra
authors: S
authors: Tremblay
authors: Michel
authors: M
authors: Simonneau
authors: Barbara
authors: B
authors: Bardoni
authors: Edouard W
authors: EW
authors: Khandjian
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism*; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D016547:Kinesins / Q000378:metabolism*; D051379:Mice; D008870:Microtubules / Q000378:metabolism; D046912:Multiprotein Complexes / Q000378:metabolism*; D011485:Protein Binding; D012313:RNA / Q000378:metabolism*; D051381:Rats; D014018:Tissue Distribution; D020798:Two-Hybrid System Techniques
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051860:Fragile X Messenger Ribonucleoprotein 1; D016547:Kinesins; D046912:Multiprotein Complexes; D012313:RNA; C484676:Kif3c protein, mouse