title: Developmental regulation of PSD-95 and nNOS expression in lumbar spinal cord of rats.
abstract: Postsynaptic density (PSD)-95 is originally isolated from glutamatergic synapse where it serves as a physical tether to allow neuronal nitric oxide synthase (nNOS) signaling by N-methyl-D-aspartate receptor (NMDAR) activity. Considering the physiological importance of glutamate receptor and nitric oxide (NO) during development, we examined the spatiotemporal expression of PSD-95 and nNOS in the lumbar spinal cord at a postnatal stage. Temporally, both gene and protein levels of them gradually increased with age after birth, peaked at the postnatal day 14 (P14), and then decreased to an adult level. In addition, the enhanced coimmunoprecipitations between PSD-95 and nNOS were detected in developing spinal cord. Spatially, PSD-95 staining codistributed with nNOS in NeuN-positive motor neurons and sensory neurons at P14. These findings indicate that PSD-95 and nNOS might collectively participate in spinal cord development.
authors: The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, China.
authors: Shangfeng
authors: S
authors: Gao
authors: Chun
authors: C
authors: Cheng
authors: Jian
authors: J
authors: Zhao
authors: Mengling
authors: M
authors: Chen
authors: Xin
authors: X
authors: Li
authors: Shuxian
authors: S
authors: Shi
authors: Shuqiong
authors: S
authors: Niu
authors: Jing
authors: J
authors: Qin
authors: Mudan
authors: M
authors: Lu
authors: Aiguo
authors: A
authors: Shen
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000502:physiology; D004268:DNA-Binding Proteins; D000076122:Disks Large Homolog 4 Protein; D005455:Fluorescent Antibody Technique; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008159:Lumbar Vertebrae; D008161:Lumbosacral Region; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D009046:Motor Neurons / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D009475:Neurons, Afferent / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000254:growth & development* / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism; D009435:Synaptic Transmission / Q000502:physiology
chemical_list: D004268:DNA-Binding Proteins; D000076122:Disks Large Homolog 4 Protein; C501449:Dlg4 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C492380:NeuN protein, mouse; D009687:Nuclear Proteins; D009569:Nitric Oxide; D052248:Nitric Oxide Synthase Type I