Hemocompatibility of hydrophilic antimicrobial copolymers of alkylated 4-vinylpyridine.
Quaternized poly(vinylpyridine) (PVP) is a polymer with inherent antimicrobial properties that is effective against Gram-positive bacteria, Gram-negative bacteria, viruses, and yeast cells. However, quaternized PVP has poor biocompatibility, which prevents its use in biomaterial applications. Copolymerization was examined as a method of modifying the structure to incorporate biocompatibility. Polyethyleneglycol methyl ether methacrylate (PEGMA) and hydroxyethyl methacrylate (HEMA) are polymers generally known to be biocompatible and thus were chosen as comonomers. Random copolymers of 4-vinylpyridine and PEGMA or HEMA were synthesized via free radical polymerization and quaternized with bromohexane. Copolymer biocompatibility was characterized by interaction with human red blood cells to analyze hemolysis. Hemolysis of human red blood cells was conducted on insoluble films and on water-soluble polymers in a serial dilution study. Hemolysis results demonstrated that blood compatibility does not depend on PEG chain length in PEGMA incorporated copolymers. Results indicate a critical weight ratio of PEGMA to VP in copolymers separating the no-hemolysis regime from 100% hemolysis.
Allison
Bradley C
BC
Department of Materials Engineering and Department of Food Science, Purdue University, West Lafayette, IN 47907, USA.
Applegate
Bruce M
BM
Youngblood
Jeffrey P
JP
D000890:Anti-Infective Agents / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000737:chemistry; D008954:Models, Biological; D008968:Molecular Conformation; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D008689:Methacrylates; D011108:Polymers; D011145:Polyvinyls; D011725:Pyridines; C524499:polyethylene glycol methacrylate; D014867:Water; D011092:Polyethylene Glycols; C005044:hydroxyethyl methacrylate; C029351:4-vinylpyridine