[Identification of a new Puumala hantavirus subtype in rodents from China].
In order to detect the nucleic acid of Puumala hantavirus, RNA was extracted from lungs of bank voles captured in Northeast China, and partial S and M genome segments of Puumala virus were amplified by RT-PCR and sequenced. Phylogenetic analysis suggested that Chinese Puumala virus had diverged from the common node of PUUV, with accumulating nucleotide substitutions and formed a distinct lineage from other Puumala viruses. Newly found Puumala virus was most closely related to the Kamiiso-8Cr-95 and Tobetsu-60Cr-93 strains which came from Japan and the muju strains which came from South Korea. By analysis of S and M genome segments of Puumala virus, we deduced a new Puumala virus subtype did exist in Northeast China.
Tang
Li-hua
LH
State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China. tlhfree@hotmail.com
Zhang
Quan-fu
QF
Xiu
Mei-hong
MH
Gu
Guang-wei
GW
Bo
Shen
S
Yang
Xian-da
XD
Liang
Mi-fang
MF
Li
De-xin
DX
D000818:Animals; D001483:Base Sequence; D002681:China; D002522:Chlorocebus aethiops; D019143:Evolution, Molecular; D016679:Genome, Viral; D006476:Orthohantavirus / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D029262:Puumala virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012377:Rodentia / Q000821:virology*; D017422:Sequence Analysis, DNA; D014709:Vero Cells