[Recombinant HEV caspid protein p239 specifically attached on HepG2 cells and blocked the infection of wild-type HEV on liver cells].
By using Western blot and immunofluorescence assays, the recombinant HEV capsid protein p239 was found specifically attached to the HepG2 cell surface and entered to the cytoplasm with the increase of incubation temperature. Pre-mixture of wild-type HEV with p239 blocked the infectivity of the virus on primary cultured human hepatocytes and HepG2 cells, indicating that p239 and HEV competed the same targeting site on these cells. These data provide evidence that p239 has a similar cell surface structure with wild-type HEV.
Miao
Ji
J
National Institute of Diagnostics and Vaccine Development of Infectious Disease, Xiamen University, Xiamen 361005, China.
Zheng
Zi-zheng
ZZ
He
Shui-zhen
SZ
Liu
Ping-guo
PG
Wu
Xiao-cheng
XC
Sun
Yuan-yuan
YY
Tang
Ming
M
Zhang
Jun
J
Xia
Ning-shao
NS
D015153:Blotting, Western; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005455:Fluorescent Antibody Technique; D016752:Hepatitis E virus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism / Q000821:virology*; D006801:Humans; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D036022:Capsid Proteins; D011994:Recombinant Proteins