Functional and profiling studies prove that prostate cancer upregulated neuroblastoma thymosin beta is the true human homologue of rat thymosin beta15.
A peptide with a sequence identical to rat thymosin beta(Tb)15 was reported to be upregulated in human prostate cancer. However, in this report we provide evidence that TbNB, initially identified in human neuroblastoma, is the only Tb isoform upregulated in human prostate cancer and that the Tb15 sequence is not present herein. In addition, we demonstrate that human TbNB has a higher affinity for actin in comparison to Tb4 and promotes cell migration. In combination, this experimentally validates TbNB as functional homologue of rat Tb15 in the human organism and clarifies the current composition of the human Tb family.
Dhaese
S
S
VIB Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium.
Jonckheere
V
V
Goethals
M
M
Waltregny
D
D
Vandekerckhove
J
J
Ampe
C
C
Van Troys
M
M
D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013947:Thymosin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D000099201:Thymosin beta(4)
D000199:Actins; D012333:RNA, Messenger; D013947:Thymosin; D000099201:Thymosin beta(4); C525161:thymosin beta15, rat