title: Schisandrin B decreases the sensitivity of mitochondria to calcium ion-induced permeability transition and protects against ischemia-reperfusion injury in rat hearts.
abstract: OBJECTIVE
  In order to elucidate the molecular mechanism underlying the cardioprotection afforded by schisandrin B (Sch B), the effect of Sch B treatment on the sensitivity of mitochondria to Ca2+-stimulated permeability transition (PT) was investigated in rat hearts under normal and ischemia-reperfusion (I-R) conditions.
  
  
  RESULTS
  Myocardial I-R injury caused an increase in the sensitivity of mitochondria to Ca2+-stimulated PT in vitro. The enhanced sensitivity to mitochondrial PT was associated with increases in mitochondrial Ca2+ content as well as the extent of reactive oxidant species production in vitro and cytochrome c release in vivo. The cardioprotection afforded by Sch B pretreatment against I-R-induced injury was paralleled by the decrease in the sensitivity of myocardial mitochondria to Ca2+-stimulated PT, particularly under I-R conditions.
  
  
  CONCLUSIONS
  The results suggest that Sch B treatment increases the resistance of myocardial mitochondria to Ca2+-stimulated PT and protects against I-R-induced tissue injury.
authors: Department of Biochemistry, Hong Kong University of Science and Technology, Hong Kong SAR, China.
authors: Po-Yee
authors: PY
authors: Chiu
authors: Hoi-Yan
authors: HY
authors: Leung
authors: Ada Hl
authors: AH
authors: Siu
authors: Michel Kt
authors: MK
authors: Poon
authors: Kam-Ming
authors: KM
authors: Ko
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D002316:Cardiotonic Agents / Q000302:isolation & purification / Q000494:pharmacology; D034242:Cyclooctanes / Q000302:isolation & purification / Q000494:pharmacology; D045304:Cytochromes c / Q000378:metabolism; D005638:Fruit / Q000737:chemistry; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology*; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism; D000083162:Mitochondrial Permeability Transition Pore; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D010946:Plants, Medicinal / Q000737:chemistry; D011083:Polycyclic Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D032141:Schisandra / Q000737:chemistry
chemical_list: D002316:Cardiotonic Agents; D034242:Cyclooctanes; D017705:Lignans; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D011083:Polycyclic Compounds; D017382:Reactive Oxygen Species; C015499:schizandrin B; D045304:Cytochromes c; D002118:Calcium