Pseudovitamin B(12) is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions.
We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B(12) activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear magnetic resonance spectroscopy has enabled us to identify the compound as Coalpha-[alpha-(7-adenyl)]-Cobeta-cyanocobamide or pseudovitamin B(12). This molecule differs from cobalamin in the alpha-ligand, where it has adenine instead of 5,6-dimethylbenzimidazole bound in a alpha-glycosidic linkage to C-1 of ribose. L. reuteri is the first lactic acid bacterium in which the production of a cobalamin-like molecule has been identified and the first microorganism reported to produce exclusively pseudo-B(12).
Santos
Filipe
F
Kluyver Centre for Genomics of Industrial Fermentation, TI Food and Nutrition, and NIZO Food Research, Kernhemseweg 2, P.O. Box 20, 6710 BA Ede, The Netherlands.
Vera
José L
JL
Lamosa
Pedro
P
de Valdez
Graciela F
GF
de Vos
Willem M
WM
Santos
Helena
H
Sesma
Fernando
F
Hugenholtz
Jeroen
J
D000693:Anaerobiosis; D052200:Limosilactobacillus reuteri / Q000201:enzymology / Q000378:metabolism*; D013058:Mass Spectrometry; D019906:Nuclear Magnetic Resonance, Biomolecular; D014805:Vitamin B 12 / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification
C005602:pseudovitamin B12; D014805:Vitamin B 12