Therapeutic efficacy of DNA-loaded PLGA microspheres in tumor-bearing mice.
In vivo gene therapy was attempted using poly-(D,L-lactic-co-glycolic acid) microspheres containing the interleukin-12 gene (p2CMVmlL12) in colon adenocarcinoma (CT-26)-bearing Balb/ c mice. Treatment with IL-12-loaded microspheres inhibited tumor growth significantly compared to a control group treated with free IL-12 DNA, though the degree of tumor inhibition was not dependent on the amount of IL-12 gene loaded. Combining gene therapy with 5-fluorouracil (50 mg/kg) treatment further inhibited tumor growth compared with gene therapy alone. The results indicate that microsphere formulations of pDNA may provide an efficient gene delivery system.
Son
Hye-Jung
HJ
College of Pharmacy and Research Institute of Pharmaceutical Sciences (RIPS), Sookmyung Women's University, Seoul, Korea.
Kim
Jin-Seok
JS
D000230:Adenocarcinoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000627:therapeutic use; D045744:Cell Line, Tumor; D003131:Combined Modality Therapy; D004247:DNA / Q000008:administration & dosage*; D004337:Drug Carriers / Q000737:chemistry*; D005472:Fluorouracil / Q000627:therapeutic use; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D018664:Interleukin-12 / Q000096:biosynthesis / Q000235:genetics*; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D009368:Neoplasm Transplantation; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D016896:Treatment Outcome
D000964:Antimetabolites, Antineoplastic; D004337:Drug Carriers; D011108:Polymers; D018664:Interleukin-12; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004247:DNA; D005472:Fluorouracil