Labeling of mesenchymal stem cells by bioconjugated quantum dots.
Long-term labeling of stem cells during self-replication and differentiation benefits investigations of development and tissue regeneration. We report the labeling of human mesenchymal stem cells (hMSCs) with RGD-conjugated quantum dots (QDs) during self-replication, and multilineage differentiations into osteogenic, chondrogenic, and adipogenic cells. QD-labeled hMSCs remained viable as unlabeled hMSCs from the same subpopulation. These findings suggest the use of bioconjugated QDs as an effective probe for long-term labeling of stem cells.
Shah
Bhranti S
BS
College of Dental Medicine, Department of Biomedical Engineering, Fu Foundation School of Engineering and Applied Sciences, Columbia University, New York, New York 10032, USA.
Clark
Paul A
PA
Moioli
Eduardo K
EK
Stroscio
Michael A
MA
Mao
Jeremy J
JJ
D002478:Cells, Cultured; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009842:Oligopeptides; D045663:Quantum Dots; D013194:Staining and Labeling / Q000379:methods
D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid