[Role of the carbohydrate-binding center of bacterial lectin in the effect on adipocytes under the influence of increased temperature].
The effect of temperature and lectin from bacteria of the genues Azospirillum with blocked activity on human adipose tissue cells has been studied. The temperature used was 43.5 +/- 0.5 degrees C. Comparative results are given for the effect of lectin with the blocked and active carbohydrate-binding centers on adipocytes during heating, and the time course of the structural changes of adipocytes is described. When lectin with the active carbohydrate-binding centers was used for treatment, the heat-treated cells of a healthy obesity-prone subject died on the average in 55 +/- 5 min, whereas cells treated with lectin with the L-fucose-blocked carbohydrate-binding centers died in 80 +/- 5 min. The heat-treated cells of a diabetic obesity-prone patient died in 150 +/- 10 min on average when exposed to both active and inactive lectin. Consequently, when the lectin center is blocked with L-fucose, the effectiveness of lectin action on adipose cells of healthy obesity-prone persons decreases.
Cherkasova
O A
OA
Tuchin
V V
VV
Ponomareva
E G
EG
Nikitina
V E
VE
D017667:Adipocytes / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D016949:Azospirillum / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry / Q000494:pharmacology*; D001665:Binding Sites / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005643:Fucose / Q000494:pharmacology*; D006358:Hot Temperature; D006801:Humans; D037102:Lectins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D009765:Obesity / Q000378:metabolism* / Q000473:pathology; D050151:Subcutaneous Fat / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
D001426:Bacterial Proteins; D037102:Lectins; D005643:Fucose