[Effect of platelet-rich plasma and latissimus dorsi myofascia with blood vessel on vascularization of tissue engineered bone in dogs].
OBJECTIVE
To study the effect of platelet-rich plasma (PRP) and latissimus dorsi myofascia with blood vessel on vascularization of tissue engineered bone in dogs.


METHODS
Bone marrow stromal cells (BMSCs) were isolated from iliac bone of dogs. PRP was obtained from the same dog. And demineralized bone matrix (DBM) were prepared from homologuous bone. ABCD 4 areas were divided on the back of dog. PRP/BMSCs/DBM was implanted around the vessels of lattisimus dorsi muscle in the A. PRP/BMSCs/DBM wrapped by superficial fascia in the B. BMSCs/DBM was implanted around vessels of lattisimus dorsi muscle in the C. BMSCs/DBM wrapped by superficial fascia in the D area of the same dog. 4, 8, 12 weeks after implantation, gross specimen and histology examination were made.


RESULTS
Osteogenesis and blood vessel formation results were A>B>C>D area.


CONCLUSIONS
The results suggested that the PRP and latissimus dorsi myofascia with blood vessels could promote calcification and vascularization in tissue-engineered bone.
Li
Ning-yi
NY
Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital of Medical College, College of Stomatology, Qingdao University, Qingdao 266003, China.
Chen
Li-qiang
LQ
Chen
Tao
T
Jin
Xiao-ming
XM
Yuan
Rong-tao
RT
D000818:Animals; D001672:Biocompatible Materials; D001842:Bone and Bones; D004285:Dogs; D059630:Mesenchymal Stem Cells; D010012:Osteogenesis; D053657:Platelet-Rich Plasma; D064171:Superficial Back Muscles; D023822:Tissue Engineering
D001672:Biocompatible Materials