title: Association of acylated ghrelin profiles with chronic inflammatory markers in overweight and obese postmenopausal women: a MONET study.
abstract: OBJECTIVE
  Recent reports have suggested that the existence of associations between hormonal dysregulation and chronic upregulation of inflammatory markers, which may cause obesity-related disturbances. Thus, we examined whether acylated ghrelin (AcylG) and total ghrelin (TotG) levels could be associated with the following inflammatory markers: C-reactive protein (CRP), tumor necrosis factor alpha (TNF-alpha), and soluble TNF receptor 1 (sTNF-R1).
  
  
  METHODS
  Cross-sectional study consisting of 50 overweight and obese postmenopausal women.
  
  
  METHODS
  AcylG and TotG levels were assessed at 0, 60, 160, 170, and 180 min of the euglycemic/hyperinsulinemic clamp (EHC). We evaluated insulin sensitivity, body composition, and blood lipid profiles as well as fasting concentrations of CRP, TNF-alpha, and sTNF-R1.
  
  
  RESULTS
  In fasting conditions, sTNF-R1 was negatively correlated with AcylG (r = -0.48, P < 0.001) levels. In addition, AcylG/TotG was associated negatively with sTNF-R1 (r = -0.44, P = 0.002) and positively with TNF-alpha (r = 0.38, P = 0.009) values. During the EHC, TotG (at all time points) and AcylG (at 60 and 160 min) values were significantly decreased from fasting concentrations. AcylG maximal reduction and area under the curve (AUC) values were correlated to sTNF-R1 (r = -0.35, P = 0.02 and r = -0.34, P = 0.02, respectively). Meanwhile, the AcylG/TotG AUC ratio was associated negatively with sTNF-R1 (r = -0.29, P < 0.05) and positively with TNF-alpha (r = 0.36, P = 0.02). Following adjustments for total adiposity, sTNF-R1 remained correlated with fasting and maximal reduction AcylG values. Similarly, AcylG/TotG ratios remained significantly correlated with sTNF-R1 and TNF-alpha. Importantly, 23% of the variation in sTNF-R1 was independently predicted by fasting AcylG.
  
  
  CONCLUSIONS
  These results are the first to suggest that both fasting and EHC-induced AcylG profiles are correlated with fasting values of sTNF-R1, a component of the TNF-alpha system. Thus, AcylG may act, at least in part, as one mediator of chronic inflammatory activity in human obesity.
authors: Département de Nutrition, Université de Montréal, Montréal, Québec, Canada, H3T 1A8.
authors: David H
authors: DH
authors: St-Pierre
authors: Jean-Philippe
authors: JP
authors: Bastard
authors: Lise
authors: L
authors: Coderre
authors: Martin
authors: M
authors: Brochu
authors: Antony D
authors: AD
authors: Karelis
authors: Marie-Eve
authors: ME
authors: Lavoie
authors: Florin
authors: F
authors: Malita
authors: Jonathan
authors: J
authors: Fontaine
authors: Diane
authors: D
authors: Mignault
authors: Katherine
authors: K
authors: Cianflone
authors: Pascal
authors: P
authors: Imbeault
authors: Eric
authors: E
authors: Doucet
authors: Rémi
authors: R
authors: Rabasa-Lhoret
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism; D000368:Aged; D015415:Biomarkers / Q000097:blood; D003430:Cross-Sectional Studies; D005215:Fasting / Q000097:blood; D005260:Female; D054439:Ghrelin / Q000097:blood* / Q000378:metabolism; D015309:Glucose Clamp Technique; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D008875:Middle Aged; D009765:Obesity / Q000097:blood*; D050177:Overweight / Q000097:blood*; D017698:Postmenopause / Q000097:blood*; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000097:blood
chemical_list: D015415:Biomarkers; D054439:Ghrelin; D018836:Inflammation Mediators; D047888:Receptors, Tumor Necrosis Factor, Type I; D000123:Acetyltransferases