Protective effect of Aquilegia vulgaris (L.) on carbon tetrachloride-induced oxidative stress in rats.
The ethyl ether extract of A. vulgaris inhibited in vitro microsomal lipid peroxidation (IC50 58.8 microg/ml) and showed moderate ability to scavenge superoxide radicals and to chelate iron ions. The extract (100 mg/kg body weight, po) decreased uninduced and enzymatic microsomal lipid peroxidation in the liver of male rats pretreated with CCl4 (1 ml/kg body weight) by 27 and 40%, respectively. Activity of antioxidant and related enzymes (catalase and glucose-6-phosphate dehydrogenase) inhibited by CCl4 was significantly restored after administration of the extract. The extract itself significantly enhanced superoxide dismutase activity. There was no effect of the extract on hepatic glutathione level and cytochrome P450 content, both were decreased by CCl4. Neither CCl4 nor the tested extract affected activities of NADPH-cytochrome P450 reductase and two monooxygenases, aniline hydroxylase and aminopyrine n-demethylase. It can be concluded that the protective effect of the A. vulgaris extract in CCl4-induced liver injury is mediated by inhibition of microsomal lipid peroxidation and restoring activity of some antioxidant and related enzymes.
Kujawska
Malgorzata
M
Department of Toxicology, Poznan University of Medical Sciences, Dojazd 30, 60-631 Poznan, Poland. kujawska@amp.edu.pl
Jodynis-Liebert
Jadwiga
J
Ewertowska
Malgorzata
M
Adamska
Teresa
T
Matlawska
Irena
I
Bylka
Wieslawa
W
D000818:Animals; D031884:Aquilegia / Q000737:chemistry*; D002251:Carbon Tetrachloride / Q000037:antagonists & inhibitors* / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury; D004986:Ether / Q000737:chemistry; D016166:Free Radical Scavengers / Q000494:pharmacology* / Q000627:therapeutic use; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects; D008107:Liver Diseases / Q000517:prevention & control*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010088:Oxidoreductases / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats
D016166:Free Radical Scavengers; D010936:Plant Extracts; D004986:Ether; D002251:Carbon Tetrachloride; D010088:Oxidoreductases