Ruminal and intermediary metabolism of propylene glycol in lactating Holstein cows.
Four lactating Holstein cows fitted with ruminal cannulas and permanent indwelling catheters in the mesenteric artery, mesenteric vein, hepatic portal vein, and hepatic vein were used in a cross-over design to study the metabolism of propylene glycol (PG). Each cow received 2 treatments: control (no infusion) and infusion of 650 g of PG into the rumen at the time of the morning feeding. Propylene glycol was infused on the day of sampling only. Samples of arterial, portal, and hepatic blood as well as ruminal fluid were obtained at 0.5 h before feeding and at 0.5, 1.5, 2.5, 3.5, 5, 7, 9, and 11 h after feeding. Infusion of PG did not affect ruminal pH or the total concentration of ruminal volatile fatty acids, but did decrease the molar proportion of ruminal acetate. The ruminal concentrations of PG, propanol, and propanal as well as the molar proportion of propionate increased with PG infusion. The plasma concentrations of PG, ethanol, propanol, propanal, glucose, L-lactate, propionate, and insulin increased with PG and the plasma concentrations of acetate and beta-hydroxybutyrate decreased. The net portal flux of PG, propanol, and propanal increased with PG. The hepatic uptake of PG was equivalent to 19% of the intraruminal dose. When cows were dosed with PG, the hepatic extraction of PG was between 0 and 10% depending on the plasma concentration of PG, explaining the slow decrease in arterial PG. The increased net hepatic flux of L-lactate with PG could account for the entire hepatic uptake of PG, which suggests that the primary hepatic pathway for PG is oxidation to l-lactate. The hepatic uptake of propanol increased with PG, but no effects of PG on the net hepatic and net splanchnic flux of glucose were observed. Despite no effect of PG on net portal flux and net hepatic flux of propionate, the net splanchnic flux of propionate increased and the data suggest that propionate produced from hepatic metabolism of propanol is partly released to the blood. The data suggest that PG affects metabolism of the cows by 2 modes of action: 1) increased supply of l-lactate and propionate to gluconeogenesis and 2) insulin resistance of peripheral tissues induced by increased concentrations of PG and propanol as well as a decreased ratio of ketogenic to glucogenic metabolites in arterial blood plasma.
Kristensen
N B
NB
Department of Animal Health, Welfare and Nutrition, Faculty of Agricultural Sciences, University of Aarhus, DK-8830 Tjele, Denmark. nbk@agrsci.dk
Raun
B M L
BM
D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002417:Cattle / Q000378:metabolism*; D018592:Cross-Over Studies; D004435:Eating; D005260:Female; D007328:Insulin / Q000097:blood / Q000378:metabolism; D007774:Lactation / Q000378:metabolism*; D008099:Liver / Q000098:blood supply / Q000378:metabolism; D008638:Mesenteric Arteries / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D011422:Propionates / Q000378:metabolism; D019946:Propylene Glycol / Q000097:blood / Q000378:metabolism*; D012417:Rumen / Q000737:chemistry / Q000378:metabolism*; D013154:Spleen / Q000098:blood supply / Q000378:metabolism; D013997:Time Factors
D000447:Aldehydes; D007328:Insulin; D011422:Propionates; D002245:Carbon Dioxide; D019946:Propylene Glycol; C005556:propionaldehyde; D010100:Oxygen