Comparative study of LDL-cholesterol levels in Thai patients by the direct method and using the Friedewald formula.
In this study, low-density lipoprotein cholesterol (LDL-C) levels by direct measurement and estimation using the Friedewald formula, were compared among 1,016 Thai patients. The study assessed blood samples from out-patients sent to the Clinical Chemistry Laboratory, Department of Clinical Pathology, Rajvithi Hospital, Ministry of Public Health, for measurement of total cholesterol (TC), LDL-C, high-density lipoprotein cholesterol (HDL-C) and triglyceride (TG) levels, January 2004-December 2005. Patients' ages ranged 8-89 years, 573 (56.4%) were females. Linear regression analysis showed the two methods had highly significant correlation coefficients (p<0.001). Upon comparing the two methods, at TG levels of 151-200 mg/dl, bias was 18.3 mg/dl; and for TG levels of 201-300 mg/dl, bias was lower at 11.4 mg/dl; for TG levels of 301-400 mg/dl, bias increased to 20.9 mg/dl. The direct assay meets currently established analytical performance targets and may be useful for the diagnosis and management of hyperlipidemic patients. The Friedewald formula did not give a homogeneous performance when estimating LDL-C levels in samples with different TG levels.
Teerakanchana
Thirawuth
T
Clinical Chemistry Laboratory, Department of Clinical Pathology, Rajavithi Hospital, Ministry of Public Health, Bangkok, Thailand.
Puavilai
Wilai
W
Suriyaprom
Kanjana
K
Tungtrongchitr
Rungsunn
R
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000465:Algorithms; D002648:Child; D008078:Cholesterol, LDL / Q000032:analysis* / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D013785:Thailand
D008078:Cholesterol, LDL