Rapid, transcript-specific changes in splicing in response to environmental stress.
While the core splicing machinery is highly conserved between budding yeast and mammals, the absence of alternative splicing in Saccharomyces cerevisiae raises the fundamental question of why introns have been retained in approximately 5% of the 6000 genes. Because ribosomal protein-encoding genes (RPGs) are highly overrepresented in the set of intron-containing genes, we tested the hypothesis that splicing of these transcripts would be regulated under conditions in which translation is impaired. Using a microarray-based strategy, we find that, within minutes after the induction of amino acid starvation, the splicing of the majority of RPGs is specifically inhibited. In response to an unrelated stress, exposure to toxic levels of ethanol, splicing of a different group of transcripts is inhibited, while the splicing of a third set is actually improved. We propose that regulation of splicing, like transcription, can afford rapid and specific changes in gene expression in response to the environment.
Pleiss
Jeffrey A
JA
Department of Biochemistry and Biophysics, University of California, San Francisco, 600 16th Street, Genentech Hall, Room N-374, San Francisco, CA 94143-2200, USA.
Whitworth
Gregg B
GB
Bergkessel
Megan
M
Guthrie
Christine
C
D000596:Amino Acids / Q000172:deficiency; D015536:Down-Regulation / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000235:genetics*; D016681:Genome, Fungal / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D012322:RNA Precursors / Q000235:genetics* / Q000378:metabolism; D012326:RNA Splicing / Q000187:drug effects / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013379:Substrate Specificity; D013997:Time Factors
D000596:Amino Acids; D012322:RNA Precursors; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; C512912:GCN2 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases