Wen-Pi-Tang-Hab-Wu-Ling-San extract inhibits the release of inflammatory mediators from LPS-stimulated mouse macrophages.
OBJECTIVE
Wen-Pi-Tang is a traditional herbal prescription that has been used traditionally for the treatment of various inflammatory diseases, including chronic renal failure, renal injury, renal tubular cell damage and diabetic nephropathy. In this study, we investigated the pharmacological activity of modified Wen-Pi-Tang, Wen-Pi-Tang-Hab-Wu-Ling-San (WHW) extract.


METHODS
Production of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), IL-6 and NO in supernatant, mRNA expression of TNF-alpha, IL-1beta, IL-6 and iNOS, protein expression of iNOS, phosphorylation of mitogen-activated protein kinases (MAPKs) and activation of nuclear factor-kappa B in the extract were assayed.


RESULTS
We found that WHW extract had potent anti-inflammatory effects in LPS-stimulated RAW264.7 cells and primary peritoneal macrophages. WHW extract strongly inhibited the excessive production of inflammatory mediators, nitric oxide (NO), TNF-alpha (TNF-alpha), interleukin 1-beta (IL-1beta) and IL-6 in LPS-stimulated macrophages. The inhibition of inducible nitric oxide synthase (iNOS) and these cytokines resulted from the reduced expressions of mRNAs of iNOS and these cytokines, respectively. WHW extract attenuated the phosphorylation of mitogen-activated protein kinases (MAPKs), extracellular signal-regulated kinases 1 and 2 (ERK1/2) and c-Jun N-terminal kinase (JNK), as well as the activation of nuclear factor-kappa B (NF-kappaB) in LPS-stimulated RAW264.7 cells.


CONCLUSIONS
These data suggest that WHW extract may exhibit anti-inflammatory effects through the modulation of MAPK and the NF-kappaB-dependent pathway involved in inflammation.
Jung
Hyo Won
HW
Department of Herbology, College of Oriental Medicine, Dongguk University, Gyeongju 780-714, Republic of Korea.
Yoon
Cheol-Ho
CH
Kim
Young Ho
YH
Boo
Yong Chool
YC
Park
Kwon Moo
KM
Park
Yong-Ki
YK
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors / Q000235:genetics; D010766:Phosphorylation; D051996:Transcription Factor RelA / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D051996:Transcription Factor RelA; C521629:Wen-pi-tang-Hab-Wu-ling-san; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D020928:Mitogen-Activated Protein Kinases