Effect of acyl-CoA oxidase activity on the accumulation of gamma-decalactone by the yeast Yarrowia lipolytica: a factorial approach.
beta-Oxidation is a cyclic pathway involved in the degradation of lipids. In yeast, it occurs in peroxisomes and the first step is catalyzed by an acyl-CoA oxidase (Aoxp). The yeast Yarrowia lipolytica possesses several genes (POX) coding for Aoxps. This study is based on the factorial analysis of results obtained with the many POX derivative strains that have been constructed previously. The effect of interactions between Aoxps on the acyl-CoA oxidase (Aox) activity was important even at the second order. We then investigated the effect of Aox activity on growth and lactone production. Aox activity was correlated with acidification of the medium by cells and with cellular growth but not with lactone production, although Aox activity on short chains was inversely correlated with lactone accumulation. Due to the poor correlation between Aox activity and lactone production, the modeling of this parameter gave no satisfactory results but growth depending on Aox activity was modeled.
García
Erandi Escamilla
EE
Laboratoire de Génie des Procédés et Microbiologie Alimentaire, GPMA, IFR 92, Université de Bourgogne, ENSBANA, Dijon, France.
Nicaud
Jean-Marc
JM
Belin
Jean-Marc
JM
Waché
Yves
Y
D042962:Acyl-CoA Oxidase / Q000378:metabolism*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D003198:Computer Simulation; D004789:Enzyme Activation; D005163:Factor Analysis, Statistical; D007783:Lactones / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D008954:Models, Biological; D013045:Species Specificity; D025062:Yarrowia / Q000145:classification* / Q000378:metabolism*
D007783:Lactones; C109757:decan-4-olide; D042962:Acyl-CoA Oxidase