Ultrastructural analysis and identification of membrane proteins in the free-living amoeba Difflugia corona.
Syntaxin-1 and 25-kDa Synaptosome-associated Protein (SNAP-25) are present in the plasma membrane of several different secretory cell types and are involved in the exocytosis process. In this work, the free-living amoeba Difflugia corona was studied in relation to ultrastructure, structural membrane proteins, and proteins such as Syntaxin-1 and SNAP-25. Our results obtained by scanning electron microscopy in the amoeba without its theca, showed many membrane projections and several pore-like structures. Using immunocytochemistry, we found structural proteins Syntaxin-1 and SNAP-25.
Silva-Briano
Marcelo
M
Dept. of Biology, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes. Av. Universidad 940 C.P. 20100. Aguascalientes Ags, México.
Martínez-Hernández
Sandra Luz
SL
Adabache-Ortíz
Araceli
A
Ventura-Juárez
Javier
J
Salinas
Eva
E
Quintanar
J Luis
JL
D000656:Amoeba / Q000378:metabolism / Q000648:ultrastructure*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D050825:Synaptosomal-Associated Protein 25 / Q000378:metabolism; D050827:Syntaxin 1 / Q000378:metabolism
D008565:Membrane Proteins; D050825:Synaptosomal-Associated Protein 25; D050827:Syntaxin 1