Antifungal activity of chitinases from Trichoderma aureoviride DY-59 and Rhizopus microsporus VS-9.
Two chitinolytic fungal strains, Trichoderma aureoviride DY-59 and Rhizopus microsporus VS-9, were isolated from soil samples of Korea and Vietnam, respectively. DY-59 and VS-9 crude chitinases secreted by these fungi in the 0.5% swollen chitin culture medium had an optimal pH of 4 and the optimal temperatures of 40 degrees C and 60 degrees C, respectively. Enzymatic hydrolysis products from crab swollen chitin were N-acetyl-beta-D-glucosamine (GlcNAc) by DY-59 chitinase, and GlcNAc and N, N'-diacetylchitobiose (GlcNAc)2 by VS-9 chitinases. The chitinases degraded the cell wall of Fusarium solani hyphae to produce oligosaccharides, among which GlcNAc, (GlcNAc)2, and pentamer (GlcNAc)5 were identified by high-pressure liquid chromatography. DY-59 and VS-9 chitinases inhibited F. solani microconidial germination by more than 70% and 60% at final protein concentrations of 5 and 27 microg mL(-1), respectively, at 30 degrees C for 20 h treatment.
Nguyen
Nam Van
NV
Glucosamine Saccharide Materials, National Research Laboratory, Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju 500-757, South Korea.
Kim
Young-Ju
YJ
Oh
Kyung-Taek
KT
Jung
Woo-Jin
WJ
Park
Ro-Dong
RD
D000117:Acetylglucosamine / Q000378:metabolism; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology*; D002473:Cell Wall / Q000378:metabolism; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000378:metabolism / Q000494:pharmacology*; D004187:Disaccharides / Q000378:metabolism; D005670:Fusarium / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D007723:Korea; D009844:Oligosaccharides / Q000378:metabolism; D012233:Rhizopus / Q000201:enzymology* / Q000302:isolation & purification; D012988:Soil Microbiology; D013696:Temperature; D014242:Trichoderma / Q000201:enzymology* / Q000302:isolation & purification; D014744:Vietnam
D000935:Antifungal Agents; D004187:Disaccharides; D009844:Oligosaccharides; D002686:Chitin; C010913:N,N-diacetylchitobiose; D002688:Chitinases; D000117:Acetylglucosamine