The effect of vaginal candidiasis on the levels of the oxidative biomarkers in plasma and tissue samples of diabetic rats.
The aim of this study is to determine the relation between diabetes and vaginal candidiasis in terms of oxidative biomarker levels in a vaginal candidiasis model of the diabetic rats by evaluating malondialdehyde (MDA), sulphydrile groups or glutathione (RSH), and ascorbic acid (C vit) levels. All rats were randomly divided into five groups. All of the groups were observed for 21 days. In the treated diabetes groups, MDA (0.90, 0.68 nmol/ml and 3.78, 3.79 nmol/g tissue, plasma and vaginal tissue, respectively) and RSH (227, 171 nmol/100 ml 0.38, 0.37 micromol/g tissue, plasma and vaginal tissue, respectively) levels were found to be decreased while the levels of C vit were found to be increased (0.49, 0.37 micromol/l 2.39, 2.01 nmol/g tissue plasma, and vaginal tissue, respectively) (P < 0.05). In the groups of untreated diabetes, vaginal candidiasis were found to be more serious and oxidative biomarkers were found to be increased (MDA 1.30, 1.26 nmol/ml and 7.82, 2.37 nmol/g tissue and RSH 258, 145 nmol/100 ml and 0.31, 0.46 micromol/g tissue) while the antioxidant C vit levels were found to be decreased (0.24, 0.17 micromol/l 1.33, 2.66 nmol/g tissue) (P < 0.05). RSH, plasma MDA, blood glucose, and tissue MDA levels of vaginal candidiasis embedeled diabetic rats, were found to be higher than those in untreated diabetic and untreated vaginitis enbedeled rats 'P < 0.05'. Vaginal candidiasis caused oxidative stress in diabetic rats working together. Systemic oxidative stress biomarkers were found to be affected from vaginal candidiasis although it was a local mucosal infection.
Kustimur
Semra
S
Department of Microbiology, Faculty of Medicine, Gazi University, Besevler, Ankara 06500, Turkey.
Kalkanci
Ayse
A
Akbulut
Gonca
G
Gonul
Bilge
B
Bulduk
Emre
E
Aksakal
F Nur
FN
Yetkin
Ilhan
I
D000818:Animals; D001205:Ascorbic Acid / Q000097:blood / Q000378:metabolism; D015415:Biomarkers / Q000097:blood* / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D002177:Candidiasis / Q000382:microbiology / Q000503:physiopathology*; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000139:chemically induced / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D008054:Lipid Peroxides / Q000097:blood / Q000378:metabolism; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D018384:Oxidative Stress; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D014623:Vaginal Diseases / Q000382:microbiology / Q000503:physiopathology*
D015415:Biomarkers; D001786:Blood Glucose; D008054:Lipid Peroxides; D008315:Malondialdehyde; D013311:Streptozocin; D005978:Glutathione; D001205:Ascorbic Acid