Molecular phylogeny of parasitic Platyhelminthes based on sequences of partial 28S rDNA D1 and mitochondrial cytochrome c oxidase subunit I.
The phylogenic relationships existing among 14 parasitic Platyhelminthes in the Republic of Korea were investigated via the use of the partial 28S ribosomal DNA (rDNA) D1 region and the partial mitochondrial cytochrome c oxidase subunit 1 (mCOI) DNA sequences. The nucleotide sequences were analyzed by length, G + C %, nucleotide differences and gaps in order to determine the analyzed phylogenic relationships. The phylogenic patterns of the 28S rDNA D1 and mCOI regions were closely related within the same class and order as analyzed by the PAUP 4.0 program, with the exception of a few species. These findings indicate that the 28S rDNA gene sequence is more highly conserved than are the mCOI gene sequences. The 28S rDNA gene may prove useful in studies of the systematics and population genetic structures of parasitic Platyhelminthes.
Lee
Soo-Ung
SU
Department of Environmental and Tropical Medicine, School of Medicine, KonKuk University, Chungju, Republic of Korea. shuh@hallym.ac.kr.
Chun
Ha-Chung
HC
Huh
Sun
S
D000818:Animals; D001483:Base Sequence; D002415:Cats; D002589:Cestoda / Q000145:classification* / Q000235:genetics; D002590:Cestode Infections / Q000469:parasitology; D017124:Conserved Sequence; D017931:DNA Primers / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D003576:Electron Transport Complex IV / Q000235:genetics*; D006801:Humans; D007723:Korea; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010802:Phylogeny; D012339:RNA, Ribosomal, 28S / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017422:Sequence Analysis, DNA; D014200:Trematoda / Q000145:classification* / Q000235:genetics; D014201:Trematode Infections / Q000469:parasitology
D017931:DNA Primers; D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S; D003576:Electron Transport Complex IV