[Effects of fluid shear stress strength on mRNA expression of ATP6V1a1 in polarized osteoclasts].
OBJECTIVE
To observe effects of fluid shear stress strength on mRNA expression of ATP6V1a1 in rat polarized osteoclasts.


METHODS
Rat polarized osteoclasts suffered 0.0 (control group), 0.9, 2.9, 8.7 and 26.3 dynes/cm2 fluid shear stress for 30 min. mRNA expression of ATP6V1a1 was detected by Real-Time fluorescent quantitation PCR.


RESULTS
mRNA expression of ATP6V1a1 in 0.0 (control group), 0.9, 2.9, 8.7 and 26.3 dynes/cm2 groups was (1.14 +/- 0.06) x 10(6), (1.62 +/- 0.09) x 10(6), (2.28 +/- 0.13) x 10(6), (3.24 +/- 0.18) x 10(6), (9.16 +/- 0.53) x 10(6) copy numbers, respectively (P < 0.05).


CONCLUSIONS
In the present study, polarized osteoclasts are sensitive to fluid shear stress. mRNA expression of ATP6V1a1 has increscent tendency along with increasing of fluid shear stress strength.
Zhang
Qing-hong
QH
Dept. of Prosthodontics, The Affiliated Stomatology Hospital of Medical College, Zhejiang University, Hangzhou 310006, China.
Liang
Xing
X
Liu
Meng-tao
MT
Zhu
Bao-min
BM
Fu
Jun
J
D000818:Animals; D010010:Osteoclasts; D012333:RNA, Messenger; D051381:Rats; D033081:Shear Strength; D013314:Stress, Mechanical
D012333:RNA, Messenger