title: Correlation between amplification of the gene for the epidermal growth factor receptor (EGFR), data from preoperative proton-MR-spectroscopy (1HMRS) and histomorphometric data of glioblastomas.
abstract: OBJECTIVE
  To investigate a correlation between preoperative data from proton-MR-spectroscopy (1HMRS), genomic alterations (epidermal growth growth factor receptor [EGFR] gene amplification) and histomorphometric data from glioblastomas.
  
  
  METHODS
  In surgical specimens from 18 patients with glioblastomas, the degree of amplification of the gene for EGFR was determined in the region with the largest Ki-67 proliferation index by differential polymerase chain reaction.
  
  
  RESULTS
  Correlation analysis showed significant positive correlation between degree of EGFR gene amplification and choline and total creatine (CHO/TCR) ratio, indicating increased membrane turnover. Cases with a high EGFR/interferon ratio showed a tendency toward a low lipid peak, whereas cases with a low EGFR/interferon ratio showed a large variation of the lipid peak. Differences were observed regarding quantitative histomorphologic data of tumor cell nuclei, especially nuclear size and shape. Together with the EGFR/interferon ratio, these morphometric data provided a good reclassification of cases with low and with high values for both spectroscopic variables by means of cross-validated linear discriminant analysis.
  
  
  CONCLUSIONS
  The results provide further evidence for the biologic significance of metabolic data from preoperative 1HMRS, because these metabolic data showed a significant statistical relationship with histomorphology and a frequently occurring molecular biologic alteration (EGFR gene amplification) in glioblastomas.
authors: Department of Neuroradiology, Clinics of Johann Wolfgang Goethe-University, Frankfurt, Germany. r.nafe@em.uni-frankfurt.de
authors: Reinhold
authors: R
authors: Nafe
authors: Wolfgang
authors: W
authors: Glienke
authors: Elke
authors: E
authors: Hattingen
authors: Wolfgang
authors: W
authors: Schlote
authors: Heinrich
authors: H
authors: Lanfermann
authors: Friedhelm
authors: F
authors: Zanella
mesh_terms: D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002467:Cell Nucleus / Q000378:metabolism / Q000473:pathology; D002794:Choline / Q000378:metabolism; D003401:Creatine / Q000378:metabolism; D066246:ErbB Receptors / Q000235:genetics* / Q000378:metabolism; D005784:Gene Amplification; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008875:Middle Aged; D011300:Preoperative Care / Q000379:methods; D011522:Protons
chemical_list: D019394:Ki-67 Antigen; D011522:Protons; C512478:EGFR protein, human; D066246:ErbB Receptors; D003401:Creatine; D002794:Choline