Spontaneous transformation of a clonal population of dermis-derived multipotent cells in culture.
It is reported that adult multipotent stem cells can undergo spontaneous transformation after long-term in vitro culture. Understanding the molecular mechanisms involved in this spontaneous transformation process can help in the design of future therapeutic applications. By far, the transformation process of adult multipotent stem cell is not well understood. In this study, a tumorigenic cell line nominated TDMC1 was established from a clonal population of rat dermis-derived multipotent cells (DMCs) following spontaneous transformation in culture. The transformed cells could produce tumors with characteristics of fibrous histocytoma when they are inoculated subcutaneously into nude mice. The molecular profiles of the nontransformed DMCs and transformed cells were analyzed by a deoxyribonucleic acid microarray. Our results showed that the overactivation of the K-ras/mitogen-activated protein kinase kinase signaling pathway played an important role in the transformation process. These data may be helpful to explain, at least in part, the possible mechanism for the malignant transformation of adult multipotent cells.
Shi
Chunmeng
C
Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, People's Republic of China. shicm1010@yahoo.com.cn
Mai
Yue
Y
Zhu
Ying
Y
Cheng
Tianmin
T
Su
Yongping
Y
D000818:Animals; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002999:Clone Cells / Q000166:cytology; D020405:Dermis / Q000166:cytology* / Q000201:enzymology; D015536:Down-Regulation; D020869:Gene Expression Profiling; D051379:Mice; D008819:Mice, Nude; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000201:enzymology; D015854:Up-Regulation; D018631:ras Proteins / Q000378:metabolism
D020929:Mitogen-Activated Protein Kinase Kinases; D018631:ras Proteins