title: Efficient enrichment and desalting of protein digests using magnetic mesocellular carbon foams in matrix-assisted laser desorption/ionization mass spectrometry.
abstract: We demonstrate that magnetic mesocellular carbon foams (Mag-MCF-C) can be effectively used for enrichment and desalting of protein digests or peptides in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The large mesocellular pores and surface area of Mag-MCF-C are likely to mainly contribute to high efficiency in enrichment and desalting of protein digests. The magnetic property of Mag-MCF-C enabled easy and simple enrichment and desalting process comprising adsorption, washing, and separation steps by using an external magnet. Following elution from Mag-MCF-C by using a matrix solution (CHCA in 70% ACN/0.1% TFA), the peptides were subjected to MALDI-MS analysis. As a result, MALDI mass spectra of peptides or tryptic protein digests were distinct even at a peptide concentration as low as 50 pM. The use of Mag-MCF-C resulted in significantly improved sequence coverage for protein identification when compared to other conventional methods. Mag-MCF-C will find applications in mass spectrometric analysis of low abundance peptides or protein digests with high sensitivity.
authors: Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Korea.
authors: Young-Pil
authors: YP
authors: Kim
authors: Kun
authors: K
authors: Cho
authors: Dohoon
authors: D
authors: Lee
authors: Yunxian
authors: Y
authors: Piao
authors: Yeong Hee
authors: YH
authors: Ahn
authors: Jong Shin
authors: JS
authors: Yoo
authors: Taeghwan
authors: T
authors: Hyun
authors: Hak-Sung
authors: HS
authors: Kim
mesh_terms: D000803:Angiotensin I / Q000737:chemistry; D000818:Animals; D002384:Catalysis; D002417:Cattle; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*; D010449:Peptide Mapping; D016062:Porosity; D012235:Rhodamines / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013499:Surface Properties; D014357:Trypsin
chemical_list: C114557:5-carboxytetramethylrhodamine succinimidyl ester; D010446:Peptide Fragments; D012235:Rhodamines; D000803:Angiotensin I; D014357:Trypsin