[Evaluation of 4 culture methods of Yersinia enterocolitica].
OBJECTIVE
To establish a highly efficient culture method for detecting Yersinia enterocolitica in stool samples from diarrheic patients.


METHODS
Stool samples collected from 200 diarrheic patients were detected with a modified and 3 conventional methods, and the positivity rates of the bacterium were compared statistically.


RESULTS
With the modified method, 18 positive samples for Yersinia enterocolitica were detected from 200 stool samples, while with the 3 conventional methods, only 3, 5, and 8 positive samples were identified, respectively. Statistical analysis with McNemer test suggested significant difference in the positive detection rate between the modified and the 3 conventional methods.


CONCLUSIONS
The modified method for Yersinia enterocolitica detection has much higher sensitivity than the 3 conventional methods, and can be applied in clinical detection.
Zheng
Hao-xuan
HX
Institute of Digestive Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Sun
Yong
Y
Jiang
Bo
B
D001431:Bacteriological Techniques; D046508:Culture Techniques / Q000379:methods*; D003967:Diarrhea / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D013696:Temperature; D015008:Yersinia enterocolitica / Q000254:growth & development* / Q000302:isolation & purification