Estimating the age of immature Chrysomya albiceps (Diptera: Calliphoridae), correcting for temperature and geographical latitude.
Developmental curves for Chrysomya albiceps (Wiedemann) (Diptera: Calliphoridae) were established at 13 different constant temperatures using developmental landmarks and length as measures of age. The thermal summation constants (K) and developmental zeros (D (0)) were calculated for five developmental landmarks using the method described by Ikemoto and Takai (Environ Entomol 29:671-682, 2000). Comparison with the K and D (0) values of our findings to those of three previously published studies of C. albiceps suggests that K is directly proportional to geographic latitude, and D (0) is inversely proportional to both K and geographic latitude. Body size and developmental landmarks have a complex relationship because of trade-offs between mortality risk and female fecundity (as measured by body size) at non-optimal temperatures. This relationship can be summarized using superimposed isomorphen and isomegalen diagrams, which can then be used to make forensic estimates of postmortem intervals from larval body lengths. Finally, we recommend that future studies providing data for precise forensic estimates of postmortem intervals should use a relative temporal precision of about 10% of the total duration being measured. For many blowflies, this translates into a sampling interval of approximately every 2 h before hatching, 3 h before first ecdysis and 6 h before second ecdysis.
Richards
Cameron S
CS
Southern African Forensic Entomology Research Laboratory, Department of Zoology and Entomology, Rhodes University, Grahamstown 6140, South Africa.
Paterson
Iain D
ID
Villet
Martin H
MH
D000818:Animals; D004175:Diptera / Q000254:growth & development*; D004772:Entomology; D018732:Forensic Anthropology / Q000379:methods; D005843:Geography; D006801:Humans; D008018:Life Cycle Stages; D013696:Temperature