title: [Study on the application and evaluation of methods for gene and antigen detection in plague surveillance program].
abstract: OBJECTIVE
  To apply and evaluate new methods regarding specific gene and antigen detection in plague surveillance program.
  
  
  METHODS
  1798 samples from natural foci of plague were tested, using internal quality control multiple-polymerase chain reaction, F1 antigen marked by immuno chromatographic assay and enzyme linked immunosorbent assay. Culture of Yersinia pestis and reverse indirect hemagglutination assay were used as reference diagnostic methods.
  
  
  RESULTS
  The overall positive rate of culture on Yersinia pestis together with gene and antigen detection was 7.34%, showing an 16.81% increase when comparing to 6.28% using Yersinia pestis culture method alone. The rate of coincidence was 97.13%.
  
  
  CONCLUSIONS
  The new standard being used for specific gene and antigen detection could increase the positive rate of diagnosis on plague.
authors: State Kay Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors: Rong
authors: R
authors: Hai
authors: Dong-zheng
authors: DZ
authors: Yu
authors: Xian-ming
authors: XM
authors: Shi
authors: Zhong-bing
authors: ZB
authors: Zhang
authors: Yong-jiao
authors: YJ
authors: Tang
authors: Peng
authors: P
authors: Wang
authors: Lian-xu
authors: LX
authors: Xia
authors: Shao-zhen
authors: SZ
authors: Wei
authors: Bing
authors: B
authors: Xu
authors: Ying-xu
authors: YX
authors: Qin
authors: Zhi-kai
authors: ZK
authors: Zhang
authors: Guo-xiang
authors: GX
authors: Shi
authors: Xu Dong-lei
authors: Hong
authors: H
authors: Cai
authors: En-min
authors: EM
authors: Zhang
authors: Jian-chun
authors: JC
authors: Wei
authors: Ying-zhi
authors: YZ
authors: Geng
authors: De-hui
authors: DH
authors: Huang
authors: Bin
authors: B
authors: Zhao
authors: Li-mao
authors: LM
authors: Wang
authors: Feng-qin
authors: FQ
authors: Ma
authors: Fu
authors: F
authors: Huang
authors: Yue
authors: Y
authors: Wang
authors: Tao
authors: T
authors: Zhang
authors: Jian-hua
authors: JH
authors: Zhang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D010930:Plague / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D015010:Yersinia pestis / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins; C086725:caf1 protein, Yersinia pestis