Close relation of the O-polysaccharide structure of Escherichia coli O168 and revised structure of the O-polysaccharide of Shigella dysenteriae type 4.
The O-polysaccharide was isolated from the lipopolysaccharide of Escherichia coli O168 and studied by chemical analyses and Smith degradation along with (1)H and (13)C NMR spectroscopies. The following structure of the branched pentasaccharide repeating unit of the O-polysaccharide was established: [carbohydrate structure: see text] where 6-O-acetylation of GlcNAc is partial. Reinvestigation of the O-polysaccharide of Shigella dysenteriae type 4 established earlier showed it to have the same structure except for that the lateral Fuc residue is nonstoichiometrically O-acetylated at each position.
Perepelov
Andrei V
AV
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. perepel@ioc.ac.ru
Liu
Bin
B
Senchenkova
Sof'ya N
SN
Shashkov
Alexander S
AS
Feng
Lu
L
Knirel
Yuriy A
YA
Wang
Lei
L
D000107:Acetylation; D000942:Antigens, Bacterial / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002621:Chemistry / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D019081:O Antigens / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012762:Shigella dysenteriae / Q000378:metabolism*
D000942:Antigens, Bacterial; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D010100:Oxygen