Lack of hepatocellular CD10 along bile canaliculi is physiologic in early childhood and persistent in Alagille syndrome.
Many tissues, including hepatobiliary cells, express neutral endopeptidase (CD10), encoded by MME. Serum neutral endopeptidase activity (NEA) has been recommended as a marker of cholestasis in adults but not in children with Alagille syndrome (AGS). We investigated ontogenic and disease-related differences in the expression of CD10. CD10 was found on canalicular surfaces of hepatocytes throughout the lobule in 16 adults and in 31 children aged > or =24 months, with and without cholestasis, but not in 39 children aged <24 months, with and without cholestasis. Ten AGS children aged 2 months to 6 years lacked any canalicular CD10 expression. Cholangiocyte apices and/or intrasinusoidal granulocytes marked for CD10 in all subjects. Liver membrane fractions from a child with cholestasis aged <24 months and from 2 AGS patients aged >24 months contained reduced levels of CD10. In contrast, AGS children and all controls expressed CD10 similarly on granulocytes. MME mRNA was found in the liver of children aged <24 months and of adults, all with cholestasis, and of AGS patients. Granulocyte MME mRNA levels were similar among all study subjects; however, liver MME mRNA levels were 6- to 140-fold less than in normal adults in all cholestatic subjects, including AGS children. Methylation of the MME promoter was not detected in the liver of AGS children. In conclusion, hepatocytes in early childhood physiologically lack immunohistochemically detectable CD10. Reduced MME mRNA in AGS is not due to MME promoter methylation. Liver CD10 in childhood appears to undergo reduced synthesis or rapid degradation, which persists in AGS. Absence of CD10 expression thus may limit NEA as a marker of cholestasis in young patients and in AGS.
Byrne
Jane A
JA
Division of Gene and Cell Based Therapy, King's College London School of Medicine, London, UK.
Meara
Natalie J
NJ
Rayner
Anne C
AC
Thompson
Richard J
RJ
Knisely
A S
AS
D000293:Adolescent; D000328:Adult; D000368:Aged; D016738:Alagille Syndrome / Q000378:metabolism*; D001648:Bile Canaliculi / Q000378:metabolism*; D002462:Cell Membrane / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D002779:Cholestasis / Q000378:metabolism; D015331:Cohort Studies; D019175:DNA Methylation; D005434:Flow Cytometry; D006098:Granulocytes / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008875:Middle Aged; D015260:Neprilysin / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic
D015260:Neprilysin