Enzymatic and morphological evaluation of the hepatic cytoprotective effect of somatostatin and octreotide during extended experimental liver preservation.
Cytoprotective effects on the liver of somatostatin (ST) and octreotide (OT) have been previously described in normothermic ischemia-reperfusion models. The purpose of this study was an enzymatic and morphological assessment of hepatic cytoprotective effects during extended cold storage. Rat livers were washed in situ via the portal vein with University of Wisconsin solution (UW) UW+ST, or UW+OT. After 24 or 48 hours of cold ischemia time (CIT), livers were reperfused for 2 hours via the portal vein with oxygenated KHB at 37 degrees C using a nonrecirculating ex situ isolated perfusion system. Levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), and creatinine kinase (CK) were assessed in the perfusate during ex situ isolated reperfusion. After a 24-hour cold ischemia time (CIT) ALT, LDH, and CK levels were significantly lower (P < .05) in the UW+ST and the UW+OT livers than the UW livers. After 48-hour CIT, AST, ALT, LDH, and CK levels were significantly lower (P < .05) in the UW+ST and the UW+OT livers than the UW livers. Histopathological examination revealed mild differences after 24-hour CIT but an evidently less ischemically damage organ after 48-hour CIT. With the limitations of an in vitro model, ST and OT showed enzymatic and morphological effects during extended liver preservation.
Cascales-Sanchez
P
P
Service of General Surgery, Albacete Universitary General Hospital, Albacete, Spain.
Tomas-Gomez
A
A
Fernandez-Cornejo
V
V
Terol-Calpena
F
F
Sanchez-Del Campo
F
F
D000818:Animals; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D008111:Liver Function Tests; D015282:Octreotide / Q000494:pharmacology*; D009926:Organ Preservation / Q000379:methods*; D011169:Portal Vein / Q000502:physiology; D051381:Rats; D013004:Somatostatin / Q000494:pharmacology*
D013004:Somatostatin; D015282:Octreotide