title: 1-Phenylethynylpyrene (1-PEPy) as refined excimer forming alternative to pyrene: case of DNA major groove excimer.
abstract: 1-Phenylethynylpyrene fluorochrome was studied as meta- and para-derivatives of arabino-uridine-2'-carbamates in ss and dsDNA. 1-PEPy showed red-shifted emission and increased fluorescence quantum yield compared to pyrene. Although 1-PEPy has very short excited lifetime (<2.5 ns), it is able to form inter- and intrastrand excimers on DNA, probably resulting from spatial preorganization of two dye molecules.
authors: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997, Moscow, Russia.
authors: Irina V
authors: IV
authors: Astakhova
authors: Andrei D
authors: AD
authors: Malakhov
authors: Irina A
authors: IA
authors: Stepanova
authors: Alexey V
authors: AV
authors: Ustinov
authors: Stanislav L
authors: SL
authors: Bondarev
authors: Alexander S
authors: AS
authors: Paramonov
authors: Vladimir A
authors: VA
authors: Korshun
mesh_terms: D002241:Carbohydrates / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009025:Morpholines; D009691:Nucleic Acid Denaturation; D009705:Nucleosides / Q000737:chemistry; D010777:Photochemistry; D011721:Pyrenes / Q000737:chemistry*; D013696:Temperature
chemical_list: C525966:1-phenylethynylpyrene; D002241:Carbohydrates; D005456:Fluorescent Dyes; D009025:Morpholines; D009705:Nucleosides; D011721:Pyrenes; D004247:DNA; C038974:amorolfine