Road to precision: recombinase-based targeting technologies for genome engineering.
In the past years, recombinase-based approaches for integrating transgenes into defined chromosomal loci of mammalian cells have gained increasing attention. This method is attractive since it enables to precisely integrate transgenes of interest into pre-defined integration sites, thereby allowing to predict the expression properties of a genetically manipulated cell. This review focuses on the current state of targeting strategies including RMCE employing site-specific recombinases such as Cre, Flp and PhiC31. In particular, applications for protein expression, virus production, transgenic animals and chromosome engineering are described.
Wirth
Dagmar
D
Helmholtz Centre for Infection Research, Department of Gene Regulation and Differentiation and Model Systems for Infection and Immunity, Inhoffenstrasse 7, D-38124 Braunschweig, Germany. Dagmar.Wirth@helmholtz-hzi.de
Gama-Norton
Leonor
L
Riemer
Pamela
P
Sandhu
Upneet
U
Schucht
Roland
R
Hauser
Hansjörg
H
D000818:Animals; D001709:Biotechnology / Q000639:trends*; D018390:Gene Targeting / Q000639:trends*; D005818:Genetic Engineering / Q000639:trends*; D016678:Genome / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*; D045522:Recombinases / Q000235:genetics*
D011994:Recombinant Proteins; D045522:Recombinases