title: Measurement of cerebral blood volume in humans using hyperoxic MRI contrast.
abstract: OBJECTIVE
  To develop a new method of measuring quantitative regional cerebral blood volume (CBV) using epochs of hyperoxia as an intravenous contrast agent with T2*-weighted MRI.
  
  
  METHODS
  Images were acquired from six subjects (four male, two female, mean age 29 +/- 3.7 years) using a sequence combining pulsed arterial spin labeling interleaved with a gradient echo echo-planar imaging (EPI) blood oxygenation level-dependent (BOLD) sequence at 3T. The hyperoxia paradigm lasted 28 minutes consisting of 4 minutes of normoxia, two 6-minute blocks of hyperoxia separated by 6 minutes of normoxia. During the hyperoxic blocks the subjects were delivered a fractional oxygen concentration of 0.5.
  
  
  RESULTS
  The mean CBV was calculated to be 3.77 +/- 1.05 mL/100 g globally, 3.93 +/- 0.90 mL/100 g in gray matter (GM), and 2.52 +/- 0.78 mL/100 g in white matter (WM). The mean GM/WM ratio was thus found to be 1.56. These values are comparable to those obtained in other studies.
  
  
  CONCLUSIONS
  The hyperoxia technique for measuring CBV may be particularly useful for patient groups where an injected bolus of contrast agent is contraindicated. As more functional studies are employing epochs of inspired gases for calibration purposes, this method is easily incorporated into existing paradigms to produce a noninvasive, repeatable, easily tolerated, and quantitative measurement of regional CBV.
authors: FMRIB Centre, Department of Clinical Neurology, University of Oxford, Oxford, UK. bulte@fmrib.ox.ac.uk
authors: Daniel
authors: D
authors: Bulte
authors: Peter
authors: P
authors: Chiarelli
authors: Richard
authors: R
authors: Wise
authors: Peter
authors: P
authors: Jezzard
mesh_terms: D001784:Blood Gas Analysis; D001921:Brain / Q000473:pathology; D002138:Calibration; D002560:Cerebrovascular Circulation; D003287:Contrast Media / Q000494:pharmacology*; D017352:Echo-Planar Imaging / Q000379:methods*; D005260:Female; D006801:Humans; D018496:Hyperoxia; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D015233:Models, Statistical; D010100:Oxygen / Q000378:metabolism; D010477:Perfusion; D015203:Reproducibility of Results; D013113:Spin Labels
chemical_list: D003287:Contrast Media; D013113:Spin Labels; D010100:Oxygen