A critical role for phospholipase C in protective immunity conferred by listeriolysin O-deficient Listeria monocytogenes.
Attenuated recombinant Listeria monocytogenes (Lm) strains are a promising class of vaccine vectors that trigger protective antigen-specific CD8 T cells. Listeriolysin O (LLO) is an important Lm virulence determinant allowing the bacterium to escape from the endocytic vacuole into the cell cytoplasm in phagocytic cells. However in non-phagocytic cells, Lm phospholipase C can also mediate cytoplasmic entry. The ability of LLO-deficient Lm to confer long-term protection to infection is uncertain. Herein, we demonstrate that LLO-deficient Lm mutants can prime protective immunity to subsequent Lm infection and that Lm phospholipase C is required for protective immunity conferred by LLO-deficient Lm.
Orgun
Nural N
NN
Department of Pediatrics and Microbiology, Center for Infectious Diseases and Microbiology Translational Research, University of Minnesota School of Medicine, Minneapolis, MN 55455, USA.
Way
Sing Sing
SS
D000818:Animals; D001426:Bacterial Proteins / Q000502:physiology*; D001427:Bacterial Toxins / Q000235:genetics*; D001428:Bacterial Vaccines / Q000276:immunology*; D015169:Colony Count, Microbial; D006360:Heat-Shock Proteins / Q000235:genetics*; D006460:Hemolysin Proteins / Q000235:genetics*; D008089:Listeria monocytogenes / Q000276:immunology*; D008088:Listeriosis / Q000276:immunology / Q000517:prevention & control; D008099:Liver / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000382:microbiology; D010738:Type C Phospholipases / Q000502:physiology*; D014613:Vaccines, Attenuated / Q000276:immunology
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D014613:Vaccines, Attenuated; D010738:Type C Phospholipases; C038705:hlyA protein, Listeria monocytogenes