Covalent attachment of Candida rugosa lipase on chemically modified hybrid matrix of polysiloxane-polyvinyl alcohol with different activating compounds.
Candida rugosa lipase was immobilized by covalent binding on hybrid matrix of polysiloxane-polyvinyl alcohol chemically modified with different activating agents as glutaraldehyde, sodium metaperiodate and carbonyldiimidazole. The experimental results suggested that functional activating agents render different interactions between enzyme and support, producing consequently alterations in the optimal reaction conditions. Properties of the immobilized systems were assessed and their performance on hydrolytic and synthetic reactions were evaluated and compared with the free enzyme. In hydrolytic reactions using p-nitrophenyl palmitate as substrate all immobilized systems showed higher thermal stability and optima pH and temperature values in relation to the free lipase. Among the activating compounds, carbonyldiimidazole resulted in a total recovery of activity on the support and the highest thermal stability. For the butyl butyrate synthesis, the best performance (molar conversion of 95% and volumetric productivity of 2.33 g L(-1)h(-1)) was attained with the lipase immobilized on POS-PVA activated with sodium metaperiodate. The properties of the support and immobilized derivatives were also evaluated by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopies and chemical composition (FTIR).
Santos
Julio C
JC
Engineering School of Lorena-University of São Paulo, PO Box 116, Lorena 12-602.810, SP, Brazil.
Mijone
Patrícia D
PD
Nunes
Gisele F M
GF
Perez
Victor H
VH
de Castro
Heizir F
HF
D020001:1-Butanol / Q000378:metabolism; D020148:Butyric Acid / Q000378:metabolism; D002175:Candida / Q000187:drug effects / Q000201:enzymology*; D004602:Elements; D004789:Enzyme Activation / Q000187:drug effects; D020536:Enzyme Activators / Q000494:pharmacology*; D004795:Enzyme Stability / Q000187:drug effects; D004800:Enzymes, Immobilized / Q000648:ultrastructure; D004951:Esterification / Q000187:drug effects; D006207:Half-Life; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis / Q000187:drug effects; D008049:Lipase / Q000378:metabolism* / Q000648:ultrastructure; D011142:Polyvinyl Alcohol / Q000737:chemistry / Q000378:metabolism*; D011489:Protein Denaturation / Q000187:drug effects; D012833:Siloxanes / Q000737:chemistry / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
D004602:Elements; D020536:Enzyme Activators; D004800:Enzymes, Immobilized; D012833:Siloxanes; D020148:Butyric Acid; D020001:1-Butanol; D011142:Polyvinyl Alcohol; D008049:Lipase