title: Binding of an aptamer to the N-terminal fragment of VCAM-1.
abstract: In vitro selection of 2'-fluoropyrimidine oligonucleotide aptamers was performed against the N-terminal two-domain fragment of mouse VCAM-1. The SELEX procedure enriched the starting pool in a family of homologous sequences. High binding affinity (10nM) of one member of this family, aptamer 12.11, was demonstrated in a filter binding assay.
authors: Commissariat á l'Energie Atomique, DSV, I(2)BM, SHFJ, Laboratoire d'Imagerie Moléculaire Expérimentale, 4 place du général Leclerc, Orsay, France.
authors: Fabien
authors: F
authors: Chauveau
authors: Youssef
authors: Y
authors: Aissouni
authors: Jorg
authors: J
authors: Hamm
authors: Hervé
authors: H
authors: Boutin
authors: Domenico
authors: D
authors: Libri
authors: Frédéric
authors: F
authors: Ducongé
authors: Bertrand
authors: B
authors: Tavitian
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D001667:Binding, Competitive; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D016415:Sequence Alignment; D019010:Vascular Cell Adhesion Molecule-1 / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D010446:Peptide Fragments; D019010:Vascular Cell Adhesion Molecule-1