title: Proteomic characterization of enterohemorrhagic escherichia coli O157:H7 in the oxidation-induced viable but non-culturable state.
abstract: Enterohemorrhagic Escherichia coli (EHEC) O157 strain F2, a food isolate of an outbreak, is resistant to oxidative stress, but has increased stress-sensitivity after passage through mice. The stress-sensitive variant of F2 (designated MP37) has decreased culturability, but retains membrane integrity under stress conditions, indicating that the cells enter a viable but non-culturable (VBNC) state. Proteomic analyses revealed that MP37 in the VBNC state had decreased levels of some oxidation-responsive factors (AhpCF, AceF), but it markedly increased levels of outer membrane protein W (OmpW). Because F2 expressed higher levels of some ribosome-associated proteins (RaiA, S6, Bcp) than MP37, the effect of animal passage on the induction of the VBNC state in the EHEC O157 cells might be due to ribosomal activity.
authors: Division of Biomedical Food Research, National Institute of Health Sciences, Tokyo, Japan. hasakura@nihs.go.jp
authors: Hiroshi
authors: H
authors: Asakura
authors: Nantika
authors: N
authors: Panutdaporn
authors: Keiko
authors: K
authors: Kawamoto
authors: Shizunobu
authors: S
authors: Igimi
authors: Shigeki
authors: S
authors: Yamamoto
authors: Sou-Ichi
authors: S
authors: Makino
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D051047:Dihydrolipoyllysine-Residue Acetyltransferase / Q000378:metabolism; D004927:Escherichia coli Infections / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D029968:Escherichia coli Proteins / Q000302:isolation & purification / Q000378:metabolism*; D005516:Food Microbiology; D051379:Mice; D050296:Microbial Viability; D010084:Oxidation-Reduction; D054464:Peroxiredoxins / Q000378:metabolism; D040901:Proteomics; D012269:Ribosomal Proteins / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D012269:Ribosomal Proteins; C064035:ompW protein, E coli; D054464:Peroxiredoxins; D051047:Dihydrolipoyllysine-Residue Acetyltransferase