Identification of "Cronobacter" spp. (Enterobacter sakazakii).
A taxonomic reclassification of the neonatal pathogen Enterobacter sakazakii to consist of five species within a new genus, "Cronobacter," has recently been proposed. The correct identification of these organisms is important to clinicians. Therefore, using 312 Enterobacteriaceae, including 210 "Cronobacter" strains, the reliabilities of biochemical and genetic confirmation tests were investigated. All "Cronobacter" isolates were positive using dnaG and gluA PCR protocols, and all expressed alpha-glucosidase activity. ID32E v3.0 identified 99.5% of "Cronobacter" isolates (as the nearest match to E. sakazakii).
Iversen
Carol
C
Institute for Food Safety and Hygiene, University of Zurich, Winterthurerstrasse 272, CH-8057, Zurich, Switzerland.
Lehner
Angelika
A
Mullane
Niall
N
Marugg
John
J
Fanning
Séamus
S
Stephan
Roger
R
Joosten
Han
H
D044083:Cronobacter sakazakii / Q000145:classification* / Q000235:genetics; D019915:DNA Primase / Q000235:genetics; D005838:Genotype; D006801:Humans; D010641:Phenotype; D000520:alpha-Glucosidases / Q000235:genetics
D019915:DNA Primase; D000520:alpha-Glucosidases