[The use of polymerase chain reaction for detection of the agents of glanders and melioidosis using experimental infection].
Glanders and melioidosis are severe infectious diseases of people and animals. The causative agents of these infections refer to the potential agents of bioterrorism of group B. In this work the possibility of use of flagellin-based primers for the identification of B. mallei and B. pseudomallei and for diagnosis of experimental glanders and melioidosis was studied. The obtained results permit to make a conclusion that PCR using the developed primers may be recommended for the incorporation in the scheme of laboratory diagnosis of glanders and melioidosis both for the identification of clean cultures and in experimental clinical material.
Altukhova
V V
VV
Antonov
V A
VA
Tkachenko
G A
GA
Zinchenko
O V
OV
Zamaraev
V S
VS
Plekhanova
N G
NG
Iliukhin
V I
VI
Trofimov
D Iu
DIu
D000818:Animals; D023482:Bioterrorism; D042726:Burkholderia mallei / Q000235:genetics*; D016957:Burkholderia pseudomallei / Q000235:genetics*; D006224:Cricetinae; D005408:Flagellin / Q000235:genetics; D005896:Glanders / Q000175:diagnosis* / Q000235:genetics*; D008554:Melioidosis / Q000175:diagnosis* / Q000235:genetics*; D016133:Polymerase Chain Reaction
D005408:Flagellin