title: Control of OXPHOS efficiency by complex I in brain mitochondria.
abstract: In the present work we have analysed the efficiency (P/O ratio) of energy production by oxidative phosphorylation (OXPHOS) in rat brain, liver and heart mitochondria. This study has revealed tissue-specific differences in the mean values of P/O ratios and ATP production rates. A marked dependence of the P/O ratio on the respiration rates has been observed with complex I (NADH:ubiquinone oxidoreductase), but not with complex II (succinate dehydrogenase) respiratory substrates. The physiological impact of the P/O variations with complex I substrates has been further confirmed by extending the analysis to brain mitochondria from three independent groups of animals utilized to study the effects of dietary treatments on the age-related changes of OXPHOS. The general site-specificity of the rate-dependent P/O variability indicates that the decoupling, i.e. decreased coupling between electron transfer and proton pumping, is likely to be mostly due to slip of mitochondrial complex I. These findings suggest an additional mechanism for the pivotal role played by the energy-conserving respiratory complex I in the physiological and adaptive plasticity of mitochondrial OXPHOS.
authors: Department of Medical Biochemistry, Biology & Physics, University of Bari, Piazza G. Cesare, 70124 Bari, Italy.
authors: Tiziana
authors: T
authors: Cocco
authors: Consiglia
authors: C
authors: Pacelli
authors: Paola
authors: P
authors: Sgobbo
authors: Gaetano
authors: G
authors: Villani
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D019069:Cell Respiration / Q000502:physiology; D042967:Electron Transport Complex I / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism; D004734:Energy Metabolism / Q000502:physiology*; D005526:Food, Formulated; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D010085:Oxidative Phosphorylation; D017494:Proton Pumps / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions
chemical_list: D017494:Proton Pumps; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I