title: Caenorhabditis elegans pgp-5 is involved in resistance to bacterial infection and heavy metal and its regulation requires TIR-1 and a p38 map kinase cascade.
abstract: Animals and plants respond to bacterial infections and environmental stresses by inducing overlapping repertoires of defense genes. How the signals associated with infection and abiotic stresses are differentially integrated within a whole organism remains to be fully addressed. We show that the transcription of a Caenorhabditis elegans ABC transporter, pgp-5 is induced by both bacterial infection and heavy metal stress, but the magnitude and tissue distribution of its expression differs, depending on the type of stressor. PGP-5 contributes to resistance to bacterial infection and heavy metals. Using pgp-5 transcription as a read-out, we show that signals from both biotic and abiotic stresses are integrated by TIR-1, a TIR domain adaptor protein orthologous to human SARM, and a p38 MAP kinase signaling cassette. We further demonstrate that not all the TIR-1 isoforms are necessary for nematode resistance to infection, suggesting a molecular basis for the differential response to abiotic and biotic stress.
authors: Department of Genetics and Department of Microbiology and Immunology, M337 Always Building, 300 Pasteur Drive, Stanford University School of Medicine, Stanford, CA 94305-5120, USA.
authors: C Léopold
authors: CL
authors: Kurz
authors: Michael
authors: M
authors: Shapira
authors: Karen
authors: K
authors: Chen
authors: David L
authors: DL
authors: Baillie
authors: Man-Wah
authors: MW
authors: Tan
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000276:immunology*; D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000276:immunology*; D029742:Caenorhabditis elegans Proteins / Q000276:immunology*; D003598:Cytoskeletal Proteins / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D007113:Immunity, Innate / Q000276:immunology*; D019216:Metals, Heavy / Q000494:pharmacology; D015800:Protozoan Proteins / Q000276:immunology*; D011552:Pseudomonas Infections / Q000276:immunology*; D043562:Receptors, G-Protein-Coupled; D048051:p38 Mitogen-Activated Protein Kinases / Q000276:immunology*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D029742:Caenorhabditis elegans Proteins; D003598:Cytoskeletal Proteins; C459579:EhPgp5 protein, Entamoeba histolytica; D019216:Metals, Heavy; D015800:Protozoan Proteins; D043562:Receptors, G-Protein-Coupled; C485906:tir-1 protein, C elegans; D048051:p38 Mitogen-Activated Protein Kinases