Telomerase-immortalized non-malignant human prostate epithelial cells retain the properties of multipotent stem cells.
Understanding prostate stem cells may provide insight into the origin of prostate cancer. Primary cells have been cultured from human prostate tissue but they usually survive only 15-20 population doublings before undergoing senescence. We report here that RC-170N/h/clone 7 cells, a clonal cell line from hTERT-immortalized primary non-malignant tissue-derived human prostate epithelial cell line (RC170N/h), retain multipotent stem cell properties. The RC-170N/h/clone 7 cells expressed a human embryonic stem cell marker, Oct-4, and potential prostate epithelial stem cell markers, CD133, integrin alpha2beta1(hi) and CD44. The RC-170N/h/clone 7 cells proliferated in KGM and Dulbecco's Modified Eagle Medium with 10% fetal bovine serum and 5 microg/ml insulin (DMEM+10% FBS+Ins.) medium, and differentiated into epithelial stem cells that expressed epithelial cell markers, including CK5/14, CD44, p63 and cytokeratin 18 (CK18); as well as the mesenchymal cell markers, vimentin, desmin; the neuron and neuroendocrine cell marker, chromogranin A. Furthermore the RC170 N/h/clone 7 cells differentiated into multi tissues when transplanted into the sub-renal capsule and subcutaneously of NOD-SCID mice. The results indicate that RC170N/h/clone 7 cells retain the properties of multipotent stem cells and will be useful as a novel cell model for studying the mechanisms of human prostate stem cell differentiation and transformation.
Li
Hongzhen
H
Center for Prostate Disease Research, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
Zhou
Jianjun
J
Miki
Jun
J
Furusato
Bungo
B
Gu
Yongpeng
Y
Srivastava
Shiv
S
McLeod
David G
DG
Vogel
Jonathan C
JC
Rhim
Johng S
JS
D000368:Aged; D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002461:Cell Line, Transformed; D019070:Cell Lineage / Q000187:drug effects / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D016922:Cellular Senescence / Q000187:drug effects / Q000235:genetics; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006085:Graft Survival / Q000187:drug effects / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D016513:Mice, SCID; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010641:Phenotype; D011467:Prostate / Q000166:cytology / Q000378:metabolism*; D033581:Stem Cell Transplantation / Q000379:methods; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D014183:Transplantation, Heterologous / Q000502:physiology
D000943:Antigens, Differentiation; D015415:Biomarkers; D003470:Culture Media; D019098:Telomerase