title: Design and performance of a 16S rRNA-targeted oligonucleotide probe for detection of members of the genus Bdellovibrio by fluorescence in situ hybridization.
abstract: A 16S rRNA-targeted, Cy3-labeled oligonucleotide probe was designed to detect members of the genus Bdellovibrio by fluorescence in situ hybridization. Specific hybridization conditions were established; however, the detection of bdellovibrios in environmental samples required enrichment, confirming that Bdellovibrio spp. are not present in large numbers in the environment.
authors: Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada N6A 5C1.
authors: Khaled K
authors: KK
authors: Mahmoud
authors: Damian
authors: D
authors: McNeely
authors: Chelsea
authors: C
authors: Elwood
authors: Susan F
authors: SF
authors: Koval
mesh_terms: D001501:Bdellovibrio / Q000235:genetics* / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S