Renin-angiotensin system expression in the K562 human erythroleukaemic cell line.
Local renin-angiotensin system (RAS) may affect leukaemic cell production within the bone marrow microenvironment. Angiotensin-converting enzyme (ACE), renin, and angiotensin could influence leukaemogenesis. In this study, mRNA expressions of the major RAS components (ACE, renin, and angiotensinogen) in K562 human erythroleukaemia cell line have been searched by Real Time quantitative polymerase chain reaction. K562 blasts are multipotential, haematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. We observed significant expressions of ACE, renin, and angiotensinogen in K562 leukaemic blast cells. Therefore, K562 human erythroleukaemia cell line may serve as an in vitro model to elucidate the role of RAS in leukaemia and to test the effects of RAS-affecting drugs on leukaemic cellular proliferation.
Koca
Ebru
E
Hacettepe University Medical School, Departments of Internal Medicine and Haematology, Ankara, Turkey. ebrukoca@gmail.com
Haznedaroglu
Ibrahim C
IC
Acar
Kadir
K
Beyazit
Yavuz
Y
Aksu
Salih
S
Misirlioglu
Muge
M
Tuncer
Serdar
S
Sayinalp
Nilgun
N
Ozcebe
Osman I
OI
Uner
Aysegul
A
D000808:Angiotensinogen / Q000235:genetics*; D002460:Cell Line; D003199:Computer Systems; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism* / Q000473:pathology; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D012083:Renin / Q000235:genetics*; D012084:Renin-Angiotensin System
D012333:RNA, Messenger; D000808:Angiotensinogen; D007703:Peptidyl-Dipeptidase A; D012083:Renin