title: Humanized NOD/SCID/IL2Rgamma(null) mice transplanted with hematopoietic stem cells under nonmyeloablative conditions show prolonged life spans and allow detailed analysis of human immunodeficiency virus type 1 pathogenesis.
abstract: In a previous study, we demonstrated that humanized NOD/SCID/IL2Rgamma(null) (hNOG) mice constructed with human hematopoietic stem cells (HSCs) allow efficient human immunodeficiency virus type 1 (HIV-1) infection. However, HIV-1 infection could be monitored for only 43 days in the animals due to their short life spans. By transplanting HSCs without any myeloablation methods, the mice successfully survived longer than 300 days with stable engraftment of human cells. The mice showed high viremia state for more than the 3 months examined, with systemic HIV-1 infection and gradual decrease of CD4+ T cells analogous to that in humans. These capacities of the hNOG mice are very attractive for modeling mechanisms of AIDS progression and therapeutic strategy.
authors: Department of Virology, Division of Medical Science, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
authors: Satoru
authors: S
authors: Watanabe
authors: Shinrai
authors: S
authors: Ohta
authors: Misako
authors: M
authors: Yajima
authors: Kazuo
authors: K
authors: Terashima
authors: Mamoru
authors: M
authors: Ito
authors: Hideo
authors: H
authors: Mugishima
authors: Shigeyoshi
authors: S
authors: Fujiwara
authors: Kazufumi
authors: K
authors: Shimizu
authors: Mitsuo
authors: M
authors: Honda
authors: Norio
authors: N
authors: Shimizu
authors: Naoki
authors: N
authors: Yamamoto
mesh_terms: D000818:Animals; D018791:CD4 Lymphocyte Count; D004195:Disease Models, Animal; D005260:Female; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000254:growth & development*; D018380:Hematopoietic Stem Cell Transplantation; D051379:Mice; D018345:Mice, Knockout; D016513:Mice, SCID; D014766:Viremia