title: Polylactosamine on glycoproteins influences basal levels of lymphocyte and macrophage activation.
abstract: beta1,3-N-acetylglucosaminyltransferase 2 (beta3GnT2) is a polylactosamine synthase that synthesizes a backbone structure of carbohydrate structures onto glycoproteins. Here we generated beta3GnT2-deficient (beta3GnT2(-/-)) mice and showed that polylactosamine on N-glycans was markedly reduced in their immunological tissues. In WT mice, polylactosamine was present on CD28 and CD19, both known immune costimulatory molecules. However, polylactosamine levels on these molecules were reduced in beta3GnT2(-/-) mice. beta3GnT2(-/-) T cells lacking polylactosamine were more sensitive to the induction of intracellular calcium flux on stimulation with anti-CD3epsilon/CD28 and proliferated more strongly than T cells from WT mice. beta3GnT2(-/-) B cells also showed hyperproliferation on BCR stimulation. Macrophages from beta3GnT2(-/-) mice had higher cell surface CD14 levels and enhanced responses to endotoxin. These results indicate that polylactosamine on N-glycans is a putative immune regulatory factor presumably suppressing excessive responses during immune reactions.
authors: Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Central-2 OSL, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan.
authors: Akira
authors: A
authors: Togayachi
authors: Yuko
authors: Y
authors: Kozono
authors: Hiroyasu
authors: H
authors: Ishida
authors: Sumie
authors: S
authors: Abe
authors: Nami
authors: N
authors: Suzuki
authors: Yuki
authors: Y
authors: Tsunoda
authors: Kozue
authors: K
authors: Hagiwara
authors: Atsushi
authors: A
authors: Kuno
authors: Takashi
authors: T
authors: Ohkura
authors: Nobuo
authors: N
authors: Sato
authors: Takashi
authors: T
authors: Sato
authors: Jun
authors: J
authors: Hirabayashi
authors: Yuzuru
authors: Y
authors: Ikehara
authors: Kouichi
authors: K
authors: Tachibana
authors: Hisashi
authors: H
authors: Narimatsu
mesh_terms: D000606:Amino Sugars / Q000172:deficiency; D000818:Animals; D018941:Antigens, CD19 / Q000276:immunology; D018106:CD28 Antigens / Q000276:immunology; D018551:Solanum lycopersicum; D008213:Lymphocyte Activation / Q000235:genetics*; D008262:Macrophage Activation / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout; D017351:N-Acetylglucosaminyltransferases / Q000172:deficiency*; D037121:Plant Lectins / Q000276:immunology; D011134:Polysaccharides / Q000172:deficiency; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000606:Amino Sugars; D018941:Antigens, CD19; D018106:CD28 Antigens; D037121:Plant Lectins; D011134:Polysaccharides; D011948:Receptors, Antigen, T-Cell; C066929:polylactosamine; D017351:N-Acetylglucosaminyltransferases; C428132:B3GNT2 protein, human