title: Functional expression of amine oxidase from Aspergillus niger (AO-I) in Saccharomyces cerevisiae.
abstract: The aim of this work was to prepare recombinant amine oxidase from Aspergillus niger after overexpressing in yeast. The yeast expression vector pDR197 that includes a constitutive PMA1 promoter was used for the expression in Saccharomyces cerevisiae. Recombinant amine oxidase was extracted from the growth medium of the yeast, purified to homogeneity and identified by activity assay and MALDI-TOF peptide mass fingerprinting. Similarity search in the newly published A. niger genome identified six genes coding for copper amine oxidase, two of them corresponding to the previously described enzymes AO-I a methylamine oxidase and three other genes coding for FAD amine oxidases. Thus, A. niger possesses an enormous metabolic gear to grow on amine compounds and thus support its saprophytic lifestyle.
authors: Department of Biochemistry, Palacký University, Slechtitelů 11, 783 71, Olomouc, Czech Republic.
authors: Katerina
authors: K
authors: Kolaríková
authors: Petr
authors: P
authors: Galuszka
authors: Iva
authors: I
authors: Sedlárová
authors: Marek
authors: M
authors: Sebela
authors: Ivo
authors: I
authors: Frébort
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000588:Amines / Q000378:metabolism; D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics; D002848:Chromatography, DEAE-Cellulose; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D000588:Amines; D005656:Fungal Proteins; D011994:Recombinant Proteins; C007940:hexylamine; D006631:Amine Oxidase (Copper-Containing)