Energy transfer between CdSe/ZnS core/shell quantum dots and fluorescent proteins.
Fluorescent proteins from the green fluorescent protein (GFP) family interact strongly with CdSe/ZnS quantum dots. Photoluminescence of GFP5 is suppressed by red-emitting CdSe/ZnS quantum dots with high efficiency in a pH-dependent manner. The elevated degree of quenching, around 90%, makes it difficult to analyze the remaining signal, and it is not clear yet whether FRET is the reason behind the quenching. When the donor is a green-emitting CdSe/ZnS quantum dot and the acceptor is the HcRed1 protein, it is possible to detect quenching of the donor and sensitized emission from the acceptor. It was verified that the sensitized emission has the low anisotropy characteristic of FRET. The present characterization identifies donor-acceptor pairs formed by fluorescent proteins and CdSe/ZnS quantum dots that are suitable for the exploration of cellular events. These donor-acceptor pairs take advantage of the exceptional photochemical properties of quantum dots allied with the unique ability of fluorescent proteins to act as gene-based fluorescent probes.
Hering
Vitor R
VR
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil. vrhering@uol.com.br
Gibson
Gary
G
Schumacher
Robert I
RI
Faljoni-Alario
Adelaide
A
Politi
Mário J
MJ
D019187:Cadmium Compounds / Q000737:chemistry*; D002942:Circular Dichroism; D031541:Fluorescence Resonance Energy Transfer; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010777:Photochemistry; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry*
D019187:Cadmium Compounds; D018036:Selenium Compounds; D049452:Green Fluorescent Proteins; C058667:cadmium selenide