Juvenile tilapia (Oreochromis mossambicus) strive to maintain physiological functions after waterborne copper exposure.
Juvenile tilapia were acutely exposed to 0.2 and 2 mg/L Cu(2+) for up to 144 h. The Na(+)-K(+)-ATPase (NKA)-specific activity in the gills of tilapia exposed to 0.2 mg/L Cu(2+) significantly decreased over 48-72 h and was restored to the control level after 96 h, but was again depressed during 120-144 h. The whole-body Cl(-) levels significantly decreased after 48 h, but recovered shortly afterwards and continued to do so until 144 h with 0.2 mg/L Cu exposure. During 48-72 h, the numbers of the wavy-convex type of mitochondria-rich (MR) cells appeared to significantly increase and the cortisol content also significantly increased. Changes in MR cell morphology might be necessary in order to enhance Cl(-) uptake, and this might be related to changes in cortisol levels. Whole-body Na(+) concentrations had significantly decreased by 72 h, but recovered during 96-144 h. Whole-body Cu(2+) concentrations also significantly increased compared to the initial concentration during 72-144 h of Cu exposure. All measured parameters (NKA activity, Na(+) concentration, and MR cell numbers) significantly decreased in fish exposed to 2 mg/L Cu, and no recovery was observed. These data demonstrate that juvenile tilapia strived to maintain physiological functions after exposure to sub-lethal concentrations of Cu.
Wu
Su Mei
SM
Department of Aquatic Biosciences, National Chiayi University, 300 University Road, Chiayi, 600, Taiwan. sumei@mail.ncyu.edu.tw
Ding
Hui Ru
HR
Lin
Li-Yih
LY
Lin
Yu-Siang
YS
D000818:Animals; D001934:Branchial Region / Q000648:ultrastructure; D002712:Chlorides / Q000378:metabolism; D003300:Copper / Q000493:pharmacokinetics / Q000633:toxicity*; D005880:Gills / Q000378:metabolism / Q000648:ultrastructure; D006854:Hydrocortisone / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D017210:Tilapia / Q000502:physiology*; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity*
D002712:Chlorides; D014874:Water Pollutants, Chemical; D003300:Copper; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D006854:Hydrocortisone