Amino acid toxicities of Escherichia coli that are prevented by leucyl-tRNA synthetase amino acid editing.
Leucyl-tRNA synthetase (LeuRS) has evolved an editing function to clear misactivated amino acids. An Escherichia coli-based assay was established to identify amino acids that compromise the fidelity of LeuRS and translation. Multiple nonstandard as well as standard amino acids were toxic to the cell when LeuRS editing was inactivated.
Karkhanis
Vrajesh A
VA
Department of Biochemistry, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave., 419 Roger Adams Laboratory, Box B-4, Urbana, Illinois 61801-3732, USA.
Mascarenhas
Anjali P
AP
Martinis
Susan A
SA
D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity*; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D007935:Leucine-tRNA Ligase / Q000378:metabolism*
D000596:Amino Acids; D029968:Escherichia coli Proteins; D007935:Leucine-tRNA Ligase