Effects of protease inhibitors on mediator preservation in the supernatant of induced sputum.
Addition of protease inhibitors in induced sputum samples may help improve the recovery of mediators. The effect of protease inhibitors, in induced sputum, on the measurement of fibronectin, VEGF, IL-5, IL-6, and IL-8 was assessed. Protease inhibitors were added to sputum supernatant of atopic asthmatic subjects. Mediators were measured by ELISA or EIA. No differences were found in VEGF, IL-5, and IL-8 levels between protease inhibitors studied. Concentrations of IL-6 and fibronectin were higher when using, respectively, the commercial cocktail, and aprotinin. Protease inhibitors, if added, should be carefully chosen at the beginning of each study, to optimize the results.
St-Laurent
Julie
J
Centre de Recherche, Hôpital Laval, Institut Universitaire de Cardiologie et de Pneumologie de l'Université Laval, 2725 Chemin Ste-Foy, Québec, (Qué), Canada.
Prince
Philippe
P
Boulay
Marie-Eve
ME
Bissonnette
Elyse
E
Boulet
Louis-Philippe
LP
D000328:Adult; D001249:Asthma / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D002452:Cell Count; D005353:Fibronectins / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007378:Interleukins / Q000032:analysis*; D011480:Protease Inhibitors / Q000494:pharmacology*; D013183:Sputum / Q000737:chemistry* / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*
D015415:Biomarkers; D005353:Fibronectins; D007378:Interleukins; D011480:Protease Inhibitors; D042461:Vascular Endothelial Growth Factor A