Analysis of virulence and growth of a purine auxotrophic mutant of Xanthomonas oryzae pathovar oryzae.
Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight of rice. A random insertional mutant library of Xoo KACC10331 was constructed using a Tn5-derived transposon, and the virulence of the mutants against the susceptible rice cultivar IR24 was assayed. After the virulence assay, the M793 (purD::Tn5) mutant that had reduced virulence against the rice plants was isolated. Thermal asymmetric interlaced-PCR and sequence analysis revealed that the transposon was inserted into the purD gene (encodes a phosphoribosylamine-glycine ligase) of the M793 mutant. The reverse transcriptase-PCR assay revealed that the mutation of the purD gene did not affect the expression of other purine biosynthesis genes. However, the M793 mutant required exogenous purines and thiamine for growth in minimal media. These results indicate that the purD gene plays a crucial role in the growth and virulence of Xoo.
Park
Young-Jin
YJ
National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon, Korea.
Song
Eun-Sung
ES
Kim
Yeong-Tae
YT
Noh
Tae-Hwan
TH
Kang
Hee-Wan
HW
Lee
Byoung-Moo
BM
D001426:Bacterial Proteins / Q000235:genetics; D019731:Carbon-Nitrogen Ligases / Q000235:genetics; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D015870:Gene Expression; D053858:Metabolic Networks and Pathways; D016254:Mutagenesis, Insertional; D009154:Mutation; D012275:Oryza / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011687:Purines / Q000096:biosynthesis* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013831:Thiamine / Q000378:metabolism; D014774:Virulence; D014974:Xanthomonas / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D011687:Purines; D019731:Carbon-Nitrogen Ligases; C022387:phosphoribosylamine-glycine ligase; C030985:purine; D013831:Thiamine