Addressing a vascular endothelium array with blood components using underlying microfluidic channels.
Here, we show that an array of endothelial cells, addressable by an underlying microfluidic network of channels containing red blood cells, can be employed as an in vitro model of in vivo circulation to monitor cellular communication between different cell types in the drug discovery process.
Genes
Luiza I
LI
Department of Chemistry, Michigan State University, East Lansing, MI 48824, USA.
V Tolan
Nicole
N
Hulvey
Matthew K
MK
Martin
R Scott
RS
Spence
Dana M
DM
D000818:Animals; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D016285:Iloprost / Q000008:administration & dosage*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D011817:Rabbits; D046888:Tissue Array Analysis / Q000295:instrumentation* / Q000379:methods; D014665:Vasodilator Agents / Q000008:administration & dosage
D014665:Vasodilator Agents; D016285:Iloprost