title: Antioxidant properties of ganglioside micelles.
abstract: Antioxidant activity of gangliosides GM1 and GT1b in the Fenton type of reaction was investigated by EPR spectroscopy using DMPO as a spin trap. Hydroxyl radical spin adduct signal intensity was significantly reduced in the presence of gangliosides at their micellar concentrations. Mean micellar hydrodynamic diameter was not changed, whereas significant changes in negative Zeta potential values were observed as evidenced by Zetasizer Nano ZS. This study showed that the primary mode of ganglioside action was not due to direct scavenging of OH., but rather to the inhibition of hydroxyl radical formation. This phenomenon is related to the ability of ganglioside micelles to bind oppositely charged ferrous ions, thus reducing their concentration and consequently inhibiting OH. formation.
authors: Laboratory of Cell Biochemistry, Vuk Vrhovac University Clinic for Diabetes, Endocrinology and Metabolic Diseases, Zagreb, Croatia. mirjana.gavella@idb.hr
authors: Mirjana
authors: M
authors: Gavella
authors: Marina
authors: M
authors: Kveder
authors: Vaskresenija
authors: V
authors: Lipovac
authors: Darija
authors: D
authors: Jurasin
authors: Nada
authors: N
authors: Filipovi-Vincekovic
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000737:chemistry; D005609:Free Radicals; D005677:G(M1) Ganglioside / Q000737:chemistry*; D005678:G(M2) Ganglioside / Q000737:chemistry*; D005732:Gangliosides / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide; D017665:Hydroxyl Radical / Q000737:chemistry; D007501:Iron; D008823:Micelles; D008954:Models, Biological; D008956:Models, Chemical; D008968:Molecular Conformation; D010710:Phosphates / Q000737:chemistry
chemical_list: D000975:Antioxidants; C045076:Fenton's reagent; D016166:Free Radical Scavengers; D005609:Free Radicals; D005732:Gangliosides; D008823:Micelles; D010710:Phosphates; D005678:G(M2) Ganglioside; D017665:Hydroxyl Radical; D005677:G(M1) Ganglioside; D006861:Hydrogen Peroxide; D007501:Iron