Production of the endocannabinoids anandamide and 2-arachidonoylglycerol by endothelial progenitor cells.
Recent studies have highlighted the importance of paracrine growth factors as mediators of pro-angiogenic effects by endothelial progenitor cells (EPCs), but little is known about the release of lipid-based factors like endocannabinoids by EPCs. In the current study, the release of the endocannabinoids anandamide and 2-arachidonoylglycerol by distinct human EPC sub-types was measured using HPLC/tandem mass-spectrometry. Anandamide release was highest by adult blood colony-forming EPCs at baseline and they also demonstrated increased 2-arachidonoylglycerol release with TNF-alpha stimulation. Treatment of mature endothelial cells with endocannabinoids significantly reduced the induction of the pro-inflammatory adhesion molecule CD106 (VCAM-1) by TNF-alpha.
Opitz
Christiane A
CA
Krannert Institute of Cardiology, Indiana University School of Medicine, 1800 North Capitol Avenue, Room E310, Indianapolis, IN, United States.
Rimmerman
Neta
N
Zhang
Yanmin
Y
Mead
Laura E
LE
Yoder
Mervin C
MC
Ingram
David A
DA
Walker
J Michael
JM
Rehman
Jalees
J
D001095:Arachidonic Acids / Q000096:biosynthesis* / Q000494:pharmacology; D063385:Cannabinoid Receptor Modulators / Q000096:biosynthesis* / Q000494:pharmacology; D063388:Endocannabinoids; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D005989:Glycerides / Q000096:biosynthesis* / Q000494:pharmacology; D006801:Humans; D053284:Polyunsaturated Alkamides / Q000494:pharmacology; D013234:Stem Cells / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D015854:Up-Regulation; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
D001095:Arachidonic Acids; D063385:Cannabinoid Receptor Modulators; D063388:Endocannabinoids; D005989:Glycerides; D053284:Polyunsaturated Alkamides; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; C094503:glyceryl 2-arachidonate; C078814:anandamide