Spectrophotometric determination of spermine and related compounds using o-hydroxyhydroquinonephthalein and manganese(II).
A simple and highly sensitive spectrophotometric method for the determination of spermine (Spm) was established based on the ternary complex formation reaction of Spm with o-hydroxyhydroquinonephthalein (QP) as a xanthene dye and manganese(II) as a metal ion in the presence of a dispersion agent. The apparent molar absorptivity at 555 nm and the relative standard deviation of the proposed method were 1.4 x 10(5) dm(3) mol(-1) cm(-1) and 0.50% (n = 10), respectively. In the method for flow-injection analysis (FIA), which employs a single-channel flow manifold system, a good linear relationship was observed over the 2 - 20 pg microl(-1) range of Spm by direct injection.
Miyachi
Kanako
K
Department of Hospital Pharmacy School of Medicine, Kinki University, Osaka, Japan. k_miyachi49@yahoo.co.jp
Moriyama
Kenzo
K
Yamaguchi
Takako
T
Tominaga
Hiroshi
H
Kamino
Shinichiro
S
Fujita
Yoshikazu
Y
D002138:Calibration; D004396:Coloring Agents / Q000737:chemistry; D017022:Flow Injection Analysis; D006873:Hydroquinones / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D008345:Manganese / Q000737:chemistry*; D015394:Molecular Structure; D013053:Spectrophotometry / Q000379:methods*; D013096:Spermine / Q000032:analysis* / Q000737:chemistry*; D014966:Xanthenes / Q000737:chemistry
D004396:Coloring Agents; D006873:Hydroquinones; D007477:Ions; D014966:Xanthenes; C039209:2-hydroxyhydroquinonephthalein; D013096:Spermine; D008345:Manganese