title: Biomimetic PAMAM-poly(benzyl L-glutamate) amphiphiles with multi-armed architecture: synthesis, physical properties and self-assembled nanoparticles.
abstract: Biomimetic poly(amidoamine)-poly(benzyl L-glutamate) (PAMAM-PBLG) amphiphiles with multi-armed architecture were synthesized by the ring-opening polymerization (ROP) of beta-benzyl-L-glutamate N-carboxyanhydride (BLG-NCA) using primary amine-terminated PAMAM dendrimer as the macroinitiator. Both (1)H NMR and (13)C NMR demonstrated that all the primary amines of PAMAM participated in initiating the ROP of BLG-NCA monomer, and the chain length of PBLG can be adjusted linearly by the molar ratio of BLG-NCA monomer to PAMAM. These multi-armed PAMAM-PBLG amphiphiles exhibited both alpha-helix and beta-sheet conformations similar to linear analogues, while their multi-armed architecture could enhance the secondary conformation content of PBLG segments. Meanwhile, the PAMAM-PBLG amphiphiles showed weaker liquid crystalline textures than the linear analogues. Moreover, spherical nanoparticles could be generated by direct injection of these PAMAM-PBLG solutions into distilled water, and their average size (38 - 275 nm) could be adjusted through the multi-armed architecture, the PBLG composition, and the concentration of the amphiphiles. These nanoparticles were stable in aqueous solution for up to 64 days at room temperature and 16 days at 37 degrees C. Consequently, this will provide a convenient method not only to synthesize multi-armed polypeptides amphiphiles, but also to generate biodegradable and biocompatible nanoparticles with adjustable size for drug/gene release.
authors: Department of Polymer Science & Engineering, School of Chemistry and Chemical Technology, Shanghai Jiao Tong University, Shanghai 200240, P.R. China.
authors: Hui
authors: H
authors: Huang
authors: Chang-Ming
authors: CM
authors: Dong
authors: Yen
authors: Y
authors: Wei
mesh_terms: D032701:Biomimetics; D002152:Calorimetry, Differential Scanning; D050091:Dendrimers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D050091:Dendrimers; D011073:Polyamines; D011108:Polymers; D013501:Surface-Active Agents; C003172:poly-gamma-benzyl-L-glutamate; D011099:Polyglutamic Acid