title: Identification and characterization of zebrafish semaphorin 6D.
abstract: The semaphorin gene family contains a large number of secreted and transmembrane proteins; some function as repulsive and attractive cues of axon guidance during development. Here, we report cloning and characterization of zebrafish transmembrane semaphorin gene, semaphorin 6D (sema6D). Sema6D is expressed predominantly in the nervous system during embryogenesis, as determined by in situ hybridization. We also found that Sema6D binds Plexin-A1 in vitro, but not other Plexins. It induces the repulsion of dorsal root ganglion axons, but not sympathetic axons. Consequently, Sema6D might use Plexin-A1 as a receptor to repel specific types of axons during development.
authors: Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors: Masafumi
authors: M
authors: Kimura
authors: Masahiko
authors: M
authors: Taniguchi
authors: Yoshinori
authors: Y
authors: Mikami
authors: Tomoyuki
authors: T
authors: Masuda
authors: Tomoyuki
authors: T
authors: Yoshida
authors: Masayoshi
authors: M
authors: Mishina
authors: Takao
authors: T
authors: Shimizu
mesh_terms: D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D005727:Ganglia, Spinal / Q000196:embryology / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D039961:Semaphorins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D020033:Protein Isoforms; D011956:Receptors, Cell Surface; D039961:Semaphorins; D029961:Zebrafish Proteins; C526275:semaphorin 6D, zebrafish