title: A Novel sulfatide, GlcCer-I6 sulfate, from the ascidian Ciona intestinalis.
abstract: A novel sulfated glycosphingolipid, SGL-1, was isolated from the ascidian Ciona intestinalis, prepared from chloroform/methanol extracts and fractionated successively on DEAE Sephadex-A25, Florisil and Iatrobeads column chromatographies. Chemical structural analysis was performed using methylation analysis, gas-liquid chromatography, combined gas-liquid chromatography-mass spectrometry, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and (1)H-NMR spectroscopy. This chemical structure is presented as GlcCer I(6)-Sulfate. The ceramide moiety was specified by t16:0, t17:0, br,t17:0, t18:0 and br,t18:0 as sphingoids, and 2-hydroxy, saturated fatty acids as represented by docosanoic and tetracosanoic acids.
authors: Department of Bioscience and Bioinformatics, College of Information Science and Engineering, Ritsumeikan University, Kusatsu, Shiga, Japan.
authors: So
authors: S
authors: Yamada
authors: Yuki
authors: Y
authors: Matsumuro
authors: Takashi
authors: T
authors: Inoue
authors: Tomonori
authors: T
authors: Kitamura
authors: Saki
authors: S
authors: Itonori
authors: Mutsumi
authors: M
authors: Sugita
authors: Masahiro
authors: M
authors: Ito
mesh_terms: D000818:Animals; D002845:Chromatography; D002938:Ciona intestinalis / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013433:Sulfoglycosphingolipids / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: C524597:GlcCer-I6 sulfate; D013433:Sulfoglycosphingolipids; D013110:Sphingosine