title: Biological variation of total testosterone, free androgen index and bioavailable testosterone in polycystic ovarian syndrome: implications for identifying hyperandrogenaemia.
abstract: OBJECTIVE
  Hyperandrogenaemia is one of the three Rotterdam consensus diagnostic criteria for polycystic ovarian syndrome (PCOS) and may be measured by estimation of total testosterone, free androgen index (FAI) or bioavailable testosterone (BioT). The aim of this study was to compare the biological variability of total testosterone with that of the biological variability of both the FAI and BioT, to determine the least variable measurement for clinical practice.
  
  
  METHODS
  Comparative study.
  
  
  METHODS
  Blood samples were collected after an overnight fast at 4-day intervals on 10 consecutive occasions from 12 PCOS patients and 11 weight- and age-matched control women.
  
  
  METHODS
  Duplicate samples of stored serum were analysed for total testosterone, SHBG and BioT in a single batch.
  
  
  RESULTS
  The PCOS group had a significantly higher median BioT, FAI and total testosterone than controls. In both the PCOS and control groups, the intraindividual variance was small and similar for BioT and FAI. There was no significant difference between the within-subject biological coefficient of variation (CV(I)) for BioT, FAI and total testosterone. The maximum and minimum critical differences were +58% and -37% for BioT and +70% and -40% for FAI, respectively.
  
  
  CONCLUSIONS
  FAI appears to be the better diagnostic marker to distinguish hyperandrogenism in patients with PCOS, but once diagnosis has been made, all three methods should be equally good in monitoring further changes in the androgen status.
authors: Department of Medicine, University of Hull, UK. l.cho@hull.ac.uk
authors: L W
authors: LW
authors: Cho
authors: E S
authors: ES
authors: Kilpatrick
authors: V
authors: V
authors: Jayagopal
authors: M J
authors: MJ
authors: Diver
authors: S L
authors: SL
authors: Atkin
mesh_terms: D000328:Adult; D000728:Androgens / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D017588:Hyperandrogenism / Q000097:blood / Q000175:diagnosis*; D011085:Polycystic Ovary Syndrome / Q000097:blood / Q000175:diagnosis*; D013739:Testosterone / Q000097:blood*; D055815:Young Adult
chemical_list: D000728:Androgens; D013739:Testosterone