"Mycobacterium ulcerans subsp. shinshuense" isolated from a skin ulcer lesion: identification based on 16S rRNA gene sequencing.
We describe the fourth reported case involving "Mycobacterium ulcerans subsp. shinshuense." Compared to previous cases, the infection was more invasive with extensive ulceration from the elbow to the forearm. Definitive identification involved IS2404 detection, 16S rRNA gene sequencing, and analysis of the 16S rRNA gene 3'-terminal region and the virulence plasmid pMUM001.
Nakanaga
Kazue
K
Leprosy Research Center, National Institute of Infectious Diseases, Higashimurayama-Shi, Tokyo, Japan. nakanaga@nih.go.jp
Ishii
Norihisa
N
Suzuki
Koichi
K
Tanigawa
Kazunari
K
Goto
Masamichi
M
Okabe
Tsutomu
T
Imada
Hideaki
H
Kodama
Akemi
A
Iwamoto
Tomotada
T
Takahashi
Hiroshi
H
Saito
Hajime
H
D000328:Adult; D005260:Female; D006801:Humans; D019911:Mycobacterium ulcerans / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012883:Skin Ulcer / Q000382:microbiology*
D012336:RNA, Ribosomal, 16S