Elaboration and structural analysis of aquasomes loaded with indomethacin.
The aim of this study was to prepare nanoparticles in form of aquasomes with Indomethacin as a low solubility drug mode. Aquasomes charged with Indomethacin were obtained through the formation of an inorganic core of calcium phosphate covered with a Lactose film and further adsorption of the Indomethacin. Structural analyses, particle size, and morphology were evaluated by X-ray powder diffractometry, transmission electron microscopy, and scanning electron microscopy. The X-ray analysis of the samples and their observation through electronic microscopy allowed us to identify the inorganic calcium phosphate nucleus formation, as well as the layers of Lactose and Indomethacin. The particle size analysis of the aquasomes obtained with the Lactose layer and charged with the drug indicated an average particle size in the range of 60-120 nm, with a media of 90 nm. Standard deviation was 18.0234 and the standard error of the media 4.1348. The method was reproducible under the conditions used to prepare the aquasomes, such as ultrasound frequency and the moment of sonication for the formation of inorganic cores.
Rojas-Oviedo
Irma
I
Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana-Xochimilco, Calzada del Hueso 1100, Col Villa Quietud, Coyoacán, México, D.F., C.P. 04690, Mexico. irma19@correo.xoc.uam.mx
Salazar-López
Rodrigo A
RA
Reyes-Gasga
José
J
Quirino-Barreda
Carlos Tomás
CT
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D018360:Crystallography, X-Ray; D004337:Drug Carriers; D004339:Drug Compounding; D007213:Indomethacin / Q000737:chemistry*; D007785:Lactose / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D010316:Particle Size; D033362:Powder Diffraction; D015203:Reproducibility of Results; D012995:Solubility; D013010:Sonication; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002130:Calcium Phosphates; D004337:Drug Carriers; D007785:Lactose; D007213:Indomethacin