title: Development of a focused oligonucleotide-array comparative genomic hybridization chip for clinical diagnosis of genomic imbalance.
abstract: BACKGROUND
  Submicroscopic genomic imbalance underlies well-defined microdeletion and microduplication syndromes and contributes to general developmental disorders such as mental retardation and autism. Array comparative genomic hybridization (CGH) complements routine cytogenetic methods such as karyotyping and fluorescence in situ hybridization (FISH) for the detection of genomic imbalance. Oligonucleotide arrays in particular offer advantages in ease of manufacturing, but standard arrays for single-nucleotide polymorphism genotyping or linkage analysis offer variable coverage in clinically relevant regions. We report the design and validation of a focused oligonucleotide-array CGH assay for clinical laboratory diagnosis of genomic imbalance.
  
  
  METHODS
  We selected >10 000 60-mer oligonucleotide features from Agilent's eArray probe library to interrogate all subtelomeric and pericentromeric regions and 95 additional clinically relevant regions for a total of 179 loci. Sensitivity and specificity were measured for 105 patient samples, including 51 with known genomic-imbalance events, as detected by bacterial artificial chromosome-based array CGH, FISH, or multiplex ligation-dependent probe amplification.
  
  
  RESULTS
  Focused array CGH detected all known regions of genomic imbalance in 51 validation samples with 100% concordance and an excellent signal-to-noise ratio. The mean SD among log(2) ratios of all noncontrol features without copy number alteration was 0.062 (median, 0.055). Clinical testing of another 211 samples from individuals with developmental delay, unexplained mental retardation, dysmorphic features, or multiple congenital anomalies revealed genomic imbalance in 25 samples (11.9%).
  
  
  CONCLUSIONS
  This focused oligonucleotide-array CGH assay, a flexible, robust method for clinically diagnosing genetic disorders associated with genomic imbalance, offers appreciable advantages over currently available platforms.
authors: Department of Laboratory Medicine, Children's Hospital Boston, Boston, MA 02115, USA.
authors: Yiping
authors: Y
authors: Shen
authors: Mira
authors: M
authors: Irons
authors: David T
authors: DT
authors: Miller
authors: Sau Wai
authors: SW
authors: Cheung
authors: Va
authors: V
authors: Lip
authors: Xiaoming
authors: X
authors: Sheng
authors: Keith
authors: K
authors: Tomaszewicz
authors: Hong
authors: H
authors: Shao
authors: Hong
authors: H
authors: Fang
authors: Hung Siv
authors: HS
authors: Tang
authors: Mira
authors: M
authors: Irons
authors: Christopher A
authors: CA
authors: Walsh
authors: Orah
authors: O
authors: Platt
authors: James F
authors: JF
authors: Gusella
authors: Bai-Lin
authors: BL
authors: Wu
mesh_terms: D000015:Abnormalities, Multiple / Q000235:genetics; D002648:Child; D002869:Chromosome Aberrations; D002658:Developmental Disabilities / Q000235:genetics; D018628:Gene Dosage; D006801:Humans; D008607:Intellectual Disability / Q000235:genetics; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*