title: Detection by reverse transcriptase-polymerase chain reaction and molecular characterization of subtype B avian metapneumovirus isolated in Brazil.
abstract: Subtype B avian metapneumovirus (aMPV) was isolated and detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in Brazilian commercial laying chicken flocks with no history of vaccination against aMPV and presenting respiratory signs and decreased egg production. RT-PCR results from samples from three affected flocks revealed that the three isolates were subtype B. Partial sequence analysis of the G glycoprotein gene confirmed that the samples belonged to subtype B and were not of the vaccine type. Comparison of nucleotide and amino acid sequences of the G gene of the three Brazilian aMPV samples with subtype B isolates from other countries revealed 95.1% to 96.1% identity. Nucleotide sequences showed 100% identity among the Brazilian subtype B samples and 95.6% identity with the subtype B vaccine strain used in Brazil. This work describes the circulation of subtype B aMPV in Brazil and discusses its importance in terms of disease epidemiology.
authors: Department of Pathology, University of São Paulo, Avenida Professor Doutor Orlando Marques de Paiva 87, Cidade Universitária, Butantã, São Paulo, SP, Brazil.
authors: Jorge Luis
authors: JL
authors: Chacón
authors: Paulo E
authors: PE
authors: Brandão
authors: Marcos
authors: M
authors: Buim
authors: Laura
authors: L
authors: Villarreal
authors: Antonio J Piantino
authors: AJ
authors: Ferreira
mesh_terms: D000818:Animals; D001938:Brazil; D002642:Chick Embryo; D002645:Chickens / Q000821:virology*; D002522:Chlorocebus aethiops; D005814:Genes, Viral; D029121:Metapneumovirus / Q000145:classification* / Q000235:genetics; D018184:Paramyxoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D011201:Poultry Diseases / Q000175:diagnosis / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary*; D014709:Vero Cells; D014779:Virus Replication