title: An epigenetic modifier results in improved in vitro blastocyst production after somatic cell nuclear transfer.
abstract: The present study was designed to examine the effect of trichostatin A (TSA), an inhibitor of histone deacetylase, on development of porcine cloned embryos. Our results showed that treatment of cloned embryos derived from sow oocytes with 50 nM TSA for up to 24 h after the onset of activation could significantly improve blastocyst yield compared to the control (46.4+/-4.6% vs 17.7+/-4.9% for treated and untreated embryos, respectively; p<0.05), whereas similar cleavage rate and total cell number per blastocyst were observed. In order to assess if the improvement is cell line specific, three cell lines were tested, and for all cell lines an enhancement in blastocyst development compared to their corresponding control was observed. Our data demonstrate that TSA treatment after somatic cell nuclear transfer in the pig can significantly improve the in vitro blastocyst production.
authors: Section of Population Genetics and Embryology, Department of Genetic and Biotechnology, Danish Institute of Agricultural Sciences, Tjele, Denmark. yunhaizhang2005@gmail.com
authors: Yunhai
authors: Y
authors: Zhang
authors: Juan
authors: J
authors: Li
authors: Klaus
authors: K
authors: Villemoes
authors: Anette M
authors: AM
authors: Pedersen
authors: Stig
authors: S
authors: Purup
authors: Gábor
authors: G
authors: Vajta
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000379:methods*; D044127:Epigenesis, Genetic; D005260:Female; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013552:Swine
chemical_list: D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; C012589:trichostatin A; D006655:Histone Deacetylases