Bioassay chamber for angiogenesis with perfused explanted arteries and electrospun scaffolding.
OBJECTIVE
The purpose of this study was to test the hypothesis that explanted perfused arteries can serve as the initial endothelial cell culture source to evaluate the onset of angiogenesis in a cellulose acetate electrospun scaffold.


METHODS
Electrospun scaffolds with fiber diameters roughly controlled in three broad ranges: 0.01 to 0.2, 0.2 to 1, and 1 to 5 microm (Nanomed Nanotechnol Biol Med 2:37-41, 2006), were used in cell culture to determine which provides the best culture topology. This scaffold was then tested in a bioassay chamber whose cellular source was an explanted abdominal aorta from donated euthanized mice. Scaffolds were draped over a cannulated vessel perfused for 24 h. Cell viability, density, and morphology were quantified.


RESULTS
The largest fiber diameter group provided the best culture topology for human umbilical vein endothelial cells, showing high cell viability and density, and enhanced elongated cell morphology. Addition of single-walled carbon nanotubes decreased cell density significantly but chitosan heightened cell density and promoted spontaneous capillary tube like structure. Viability of endothelial cells increased with higher flow in the bioassay chamber.


CONCLUSIONS
Endothelial cells showed a growth preference towards larger diameter fibers. Addition of chitosan improved culture conditions. Thus, this study provides a proof of principle for the possibility of co-culturing tissue engineered vascular networks from a perfused explant.
Rubenstein
David
D
Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY 11794-8181, USA.
Han
Dong
D
Goldgraben
Sara
S
El-Gendi
Hebah
H
Gouma
Pelagia-Irene
PI
Frame
Mary D
MD
D000818:Animals; D001011:Aorta / Q000502:physiology; D001158:Arteries / Q000502:physiology*; D001681:Biological Assay; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D051379:Mice; D018919:Neovascularization, Physiologic; D010477:Perfusion; D023822:Tissue Engineering / Q000379:methods*; D014471:Umbilical Veins / Q000166:cytology / Q000502:physiology