Mitochondrial IV complex and brain neurothrophic derived factor responses of mice brain cortex after downhill training.
Twenty-four adult male CF1 mice were assigned to three groups: non-runners control, level running exercise (0 degrees incline) and downhill running exercise (16 degrees decline). Exercise groups were given running treadmill training for 5 days/week over 8 weeks. Blood lactate analysis was performed in the first and last exercise session. Mice were sacrificed 48 h after the last exercise session and their solei (citrate synthase activity) and brain cortices (BDNF levels and cytochrome c oxidase activity) were surgically removed and immediately stored at -80 degrees C for later analyses. Training significantly increased (P<0.05) citrate synthase activity when compared to untrained control. Blood lactate levels classified the exercise intensity as moderate to high. The downhill exercise training significantly reduced (P<0.05) brain cortex cytochrome c oxidase activity when compared to untrained control and level running exercise groups. BDNF levels significantly decreased (P<0.05) in both exercise groups.
Aguiar
Aderbal S
AS
Laboratório de Fisiologia e Bioquímica do Exercício, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense, 88806-000 Criciúma, SC, Brazil.
Tuon
Talita
T
Pinho
Cléber A
CA
Silva
Luciano A
LA
Andreazza
Ana C
AC
Kapczinski
Flávio
F
Quevedo
João
J
Streck
Emílio L
EL
Pinho
Ricardo A
RA
D000818:Animals; D001921:Brain / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002950:Citrate (si)-Synthase / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D007773:Lactates / Q000097:blood; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism; D010805:Physical Conditioning, Animal / Q000502:physiology*
D019208:Brain-Derived Neurotrophic Factor; D007773:Lactates; D003576:Electron Transport Complex IV; D002950:Citrate (si)-Synthase