Rational design of memory in eukaryotic cells.
The ability to logically engineer novel cellular functions promises a deeper understanding of biological systems. Here we demonstrate the rational design of cellular memory in yeast that employs autoregulatory transcriptional positive feedback. We built a set of transcriptional activators and quantitatively characterized their effects on gene expression in living cells. Modeling in conjunction with the quantitative characterization of the activator-promoter pairs accurately predicts the behavior of the memory network. This study demonstrates the power of taking advantage of components with measured quantitative parameters to specify eukaryotic regulatory networks with desired properties.
Ajo-Franklin
Caroline M
CM
Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
Drubin
David A
DA
Eskin
Julian A
JA
Gee
Elaine P S
EP
Landgraf
Dirk
D
Phillips
Ira
I
Silver
Pamela A
PA
D005057:Eukaryotic Cells / Q000378:metabolism* / Q000502:physiology*; D025461:Feedback, Physiological / Q000502:physiology*; D053263:Gene Regulatory Networks / Q000502:physiology*; D017930:Genes, Reporter; D030781:Organisms, Genetically Modified; D012107:Research Design; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000502:physiology
D015534:Trans-Activators