title: Semi-automated selection of DNA aptamers using magnetic particle handling.
abstract: We have developed a semi-automatic selection procedure for DNA aptamers. Employing a robotic workstation for magnetic particle handling, this method allows for a fast, reproducible, and parallelized selection of DNA aptamers. The selection protocol is designed to provide high flexibility and versatility in terms of choice of buffers and reagents, as well as stringency of selection. Using this procedure, we have successfully isolated ligand-specific, high-affinity DNA aptamers.
authors: Free University of Berlin, Berlin. wochner@rna-network.com
authors: Aniela
authors: A
authors: Wochner
authors: Birgit
authors: B
authors: Cech
authors: Marcus
authors: M
authors: Menger
authors: Volker A
authors: VA
authors: Erdmann
authors: Jörn
authors: J
authors: Glökler
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics* / Q000302:isolation & purification*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008280:Magnetics / Q000295:instrumentation*; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D010316:Particle Size; D012371:Robotics / Q000295:instrumentation* / Q000379:methods; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods
chemical_list: D052157:Aptamers, Nucleotide