title: O-Mannosyltransferase 1 in Aspergillus fumigatus (AfPmt1p) is crucial for cell wall integrity and conidium morphology, especially at an elevated temperature.
abstract: Protein O-mannosyltransferases initiate O mannosylation of secretory proteins, which are of fundamental importance in eukaryotes. In this study, the PMT gene family of the human fungal pathogen Aspergillus fumigatus was identified and characterized. Unlike the case in Saccharomyces cerevisiae, where the PMT family is highly redundant, only one member of each PMT subfamily, namely, Afpmt1, Afpmt2, and Afpmt4, is present in A. fumigatus. Mutants with a deletion of Afpmt1 are viable. In vitro and in vivo activity assays confirmed that the protein encoded by Afpmt1 acts as an O-mannosyltransferase (AfPmt1p). Characterization of the DeltaAfpmt1 mutant showed that a lack of AfPmt1p results in sensitivity to elevated temperature and defects in growth and cell wall integrity, thereby affecting cell morphology, conidium formation, and germination. In a mouse model, Afpmt1 was not required for the virulence of A. fumigatus under the experimental conditions used.
authors: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors: Hui
authors: H
authors: Zhou
authors: Hongyan
authors: H
authors: Hu
authors: Lijuan
authors: L
authors: Zhang
authors: Ruoyu
authors: R
authors: Li
authors: Haomiao
authors: H
authors: Ouyang
authors: Jia
authors: J
authors: Ming
authors: Cheng
authors: C
authors: Jin
mesh_terms: D000818:Animals; D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000235:genetics; D002473:Cell Wall / Q000201:enzymology*; D017931:DNA Primers / Q000737:chemistry; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D016681:Genome, Fungal; D008297:Male; D008364:Mannosyltransferases / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008954:Models, Biological; D010641:Phenotype; D013172:Spores, Fungal / Q000378:metabolism*; D013696:Temperature
chemical_list: D017931:DNA Primers; D008364:Mannosyltransferases; C082696:protein O-mannosyltransferase