Activity of immobilized trypsin in the layer structure of polyelectrolyte microcapsules (PEMC).
Polyelectrolyte microcapsules composed by using the LbL technique on stabilized RBC as templates were coated with up to ten layer pairs of trypsin/PSS or trypsin/alginate. The trypsin layer growth was confirmed by particle electrophoresis, confocal laser scanning microscopy, flow cytometry, and protein determination according to Lowry. In the coating series with trypsin/PSS, the amount of immobilized enzyme was larger than that with trypsin/alginate. The enzyme immobilization led to activity reduction of up to 90% compared to that of the same enzyme amount in the solution. No significant differences between the activities of trypsin immobilized in combination with PSS and with alginate were found.
Garbers
Eike
E
Institute of Transfusion Medicine, Charité-Universitätsmedizin Berlin, D-10098 Berlin, Germany.
Mitlöhner
Rita
R
Georgieva
Radostina
R
Bäumler
Hans
H
D000327:Adsorption; D000464:Alginates / Q000378:metabolism; D002214:Capsules / Q000737:chemistry* / Q000378:metabolism*; D004573:Electrolytes / Q000378:metabolism*; D004586:Electrophoresis; D004800:Enzymes, Immobilized / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D006801:Humans; D011137:Polystyrenes / Q000378:metabolism; D012996:Solutions; D014357:Trypsin / Q000378:metabolism*
D000464:Alginates; D002214:Capsules; D004573:Electrolytes; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D011137:Polystyrenes; D012996:Solutions; C003321:polystyrene sulfonic acid; D020723:Glucuronic Acid; D014357:Trypsin; D016650:Fluorescein-5-isothiocyanate