Decreased heme-oxygenase (HO)-1 in the macrophages of non-small cell lung cancer.
Reactive oxygen species (ROS) are important in the initiation and promotion of cells to neoplastic growth. Heme-oxygenase (HO)-1, the inducible form of heme-oxygenase, is a cytoprotective enzyme that plays a central role in the defence against oxidative stress and is implicated in the protection of lung tissue against exogenous oxidant exposure. We investigated whether the expression of HO-1 would be decreased in lung tumour as compared with tumour-free adjacent lung tissues. HO-1 expression was quantified by immunohistochemistry in tumour macrophages, in macrophages of tumour-free lung and in tumour cells of surgical specimens collected from 53 individuals with surgically resectable non-small cell lung cancer (NSCLC). The expression of HO-1 was decreased in tumour as compared with tumour-free lung macrophages. No correlations were observed between the expression of HO-1 and both the clinicopathological characteristics and the overall survival of the examined subjects. In conclusion, our data show that macrophages of non-small cell lung cancer exhibit impaired anti-oxidant defence mechanisms, likely mediated by HO-1. Conversely, HO-1 expression does not seem to be associated with lung tumour progression and prognosis.
Boschetto
Piera
P
Department of Experimental and Clinical Medicine, University of Ferrara, Ferrara, Italy. bsp@unife.it
Zeni
Elena
E
Mazzetti
Lucia
L
Miotto
Deborah
D
Lo Cascio
Natalina
N
Maestrelli
Piero
P
Marian
Emanuela
E
Querzoli
Patrizia
P
Pedriali
Massimo
M
Murer
Bruno
B
De Rosa
Edoardo
E
Fabbri
Leonardo M
LM
Mapp
Cristina E
CE
D014408:Biomarkers, Tumor / Q000096:biosynthesis; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D051547:Heme Oxygenase-1 / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D007558:Italy / Q000453:epidemiology; D008175:Lung Neoplasms / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D016676:Macrophages, Alveolar / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D015996:Survival Rate
D014408:Biomarkers, Tumor; D051547:Heme Oxygenase-1