[Callus induction and cultivation of Camptotheca acuminata].
OBJECTIVE
To study the callus induction from leaf, stem segments and stem segments with axillary's bud and the subculture conditions of callus in Camptotheca acuminata.


METHODS
The explants were inoculated into the media of MS with different concentrations of 6-BA, NAA and 2, 4-D by orthogonal experiment.


CONCLUSIONS
The optimal medium for callus induction was MS with 6-BA 2 mg x L(-1), NAA 2 mg x L(-1) and 2,4-D 2 mg x L(-1). It had better effects in leaf than in other explants. The induction ratio in leaf reached above 80%. The callus subcultured on the medium of MS with 6-BA 1 mg x L(-1) and 2, 4-D 1 mg x L(-1) grew vigorous and more quickly than that in other media. It was loose, friable in consistency and suitable for cell culture.
Ma
Lin
L
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China. malin@swust.edu.cn
D029769:Camptotheca / Q000187:drug effects* / Q000254:growth & development*; D003470:Culture Media / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development; D018547:Plant Stems / Q000187:drug effects / Q000254:growth & development; D010946:Plants, Medicinal / Q000187:drug effects / Q000254:growth & development; D046509:Tissue Culture Techniques / Q000379:methods
D003470:Culture Media; D010937:Plant Growth Regulators