Ultrasensitive staining-free protein detection after PAA gel electrophoresis using deep UV fluorescence.
We present the observation of separated protein bands after polyacrylamide (PAA) gel electrophoresis based on the staining-free detection of their ultra violet (UV)-induced fluorescence employing deep UV confocal fluorescence microscopy. Mixtures of the three biological compounds beta-Galactosidase (from Escherichia coli), apo-Transferrin (bovine) and bovine serum albumin (BSA) have been separated and a staining free detection limit below 80 pg (7.0 x 10(8) molecules) per band has been achieved. This corresponds to approximately 270 molecules in the detection volume for confocal microscopy.
Riaplov
Eugene
E
Institute of Physical Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
Li
Qiang
Q
Seeger
Stefan
S
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity
D011506:Proteins