title: GasBench/isotope ratio mass spectrometry: a carbon isotope approach to detect exogenous CO(2) in sparkling drinks.
abstract: A new procedure for the determination of carbon dioxide (CO(2)) (13)C/(12)C isotope ratios, using direct injection into a GasBench/isotope ratio mass spectrometry (GasBench/IRMS) system, has been developed to improve isotopic methods devoted to the study of the authenticity of sparkling drinks. Thirty-nine commercial sparkling drink samples from various origins were analyzed. Values of delta(13)C(cava) ranged from -20.30 per thousand to -23.63 per thousand, when C3 sugar addition was performed for a second alcoholic fermentation. Values of delta(13)C(water) ranged from -5.59 per thousand to -6.87 per thousand in the case of naturally carbonated water or water fortified with gas from the spring, and delta(13)C(water) ranged from -29.36 per thousand to -42.09 per thousand when industrial CO(2) was added. It has been demonstrated that the addition of C4 sugar to semi-sparkling wine (aguja) and industrial CO(2) addition to sparkling wine (cava) or water can be detected. The new procedure has advantages over existing methods in terms of analysis time and sample treatment. In addition, it is the first isotopic method developed that allows (13)C/(12)C determination directly from a liquid sample without previous CO(2) extraction. No significant isotopic fractionation was observed nor any influence by secondary compounds present in the liquid phase.
authors: Laboratorio Arbitral Agroalimentario. Ministerio de Agricultura, Pesca y Alimentación, Crta. Coruña Km 10.700, 28023 Madrid. Spain. acabanero@mapya.es
authors: Ana I
authors: AI
authors: Cabañero
authors: Tamar
authors: T
authors: San-Hipólito
authors: Mercedes
authors: M
authors: Rupérez
mesh_terms: D002245:Carbon Dioxide / Q000032:analysis*; D002247:Carbon Isotopes; D002253:Carbonated Beverages / Q000032:analysis*; D013058:Mass Spectrometry; D015203:Reproducibility of Results; D014867:Water / Q000737:chemistry; D014920:Wine / Q000032:analysis
chemical_list: D002247:Carbon Isotopes; D014867:Water; D002245:Carbon Dioxide