title: Local tissue reactions after nerve repair with ethyl-cyanoacrylate compared with epineural sutures.
abstract: Anastomosis of a nerve with cyanoacrylate following a lesion has previously been shown to indicate morphological and functional recovery to an extent comparable to that of conventional epineural sutures. In this study we examined the local tissue reactions after transection and repair of rat sciatic nerve, and compared sutures with a synthetic ethyl-cyanoacrylate adhesive. Many ED-1-immunoreactive macrophages were found accumulating on either side of the repair site whereas neurofilament labelling was less pronounced distal to the repair site seven days after reparation with cyanoacrylate compared with sutures. After six months, when reinnervation was completed, the difference in ED-1-immunoreactivity was still present but to a less extent. These results indicate that ethyl-cyanoacrylate seems to induce an increased inflammatory reaction, which may lead to accelerated Wallerian degeneration, and could therefore have benefits over conventional sutures for reconstruction of peripheral nerves.
authors: Karolinska Institutet, Department of Clinical Science and Education, Södersjukhuset, Stockholm, Sweden. thomas.landegren@ki.se
authors: Thomas
authors: T
authors: Landegren
authors: Mårten
authors: M
authors: Risling
authors: Jonas K E
authors: JK
authors: Persson
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D003487:Cyanoacrylates; D005260:Female; D019922:GAP-43 Protein / Q000378:metabolism; D051272:Glucose Transporter Type 1 / Q000378:metabolism; D007150:Immunohistochemistry; D008264:Macrophages / Q000378:metabolism; D018613:Microscopy, Confocal; D009416:Nerve Regeneration; D016900:Neurofilament Proteins / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism* / Q000601:surgery*; D013537:Sutures
chemical_list: D000911:Antibodies, Monoclonal; D003487:Cyanoacrylates; D019922:GAP-43 Protein; D051272:Glucose Transporter Type 1; D016900:Neurofilament Proteins; C495678:Slc2a1 protein, mouse