MRI detection of paramagnetic chemical exchange effects in mice kidneys in vivo.
In this report, the On resonance PARamagnetic CHemical Exchange Effects (OPARACHEE) method was implemented in vivo using WALTZ-16* as a preparation pulse with a standard spin echo sequence to detect the accumulation and clearance of the TmDOTA-4AmC(-) in mouse kidney. The performance of the technique in vivo is described in terms of the magnitude of the contrast effect versus the bolus agent concentration and signal-to-noise ratio (SNR) levels. The lowest injected concentration of TmDOTA-4AmC(-), 200 microL of a 2-mM stock solution (corresponds to approximately 0.2 mM agent in plasma), reduced the total water signal in the kidney papilla by 45% 3 min after the a bolus injection. The results show that the OPARACHEE methodology employing low-amplitude RF trains can detect paramagnetic exchanging agents in vivo.
Vinogradov
Elena
E
Department of Radiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA. evinogra@bidmc.harvard.edu
He
Huamei
H
Lubag
Angelo
A
Balschi
James A
JA
Sherry
A Dean
AD
Lenkinski
Robert E
RE
D000818:Animals; D005260:Female; D007668:Kidney / Q000033:anatomy & histology*; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D009942:Organometallic Compounds
D009942:Organometallic Compounds; C463281:thulium-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate