Association of IL-12+ DC with High CD3+CD4-DR+ lymphocyte counts in long-term HIV-infected hemophilia patients with clinically stable disease.
We investigated dendritic cell (DC) subsets as well as cellular and humoral immune parameters in long-term HIV-infected hemophilia patients with clinically stable disease. DC subsets were determined by their function to produce either IL-10 or IL-12. CD11c(+)CD83(+)CD40(+)IL-10(+) and CD11c(+)CD83(+)CD40(+)IL-12(+) DC were studied in freshly obtained blood samples of 28 HIV(+) and 15 HIV(-) patients and 39 healthy controls using four-color flow cytometry, and were analyzed in relation to blood lymphocyte subpopulation counts, proportions of IgG-coated CD4(+) blood lymphocytes, neopterin, and HIV-1 viral load in the plasma, and in vitro responses of patient lymphocytes to mitogens. Proportions and ratios of IL-10(+) DC and IL-12(+) DC were similar in HIV(+) and HIV(-) patients and healthy controls. Whereas IL-12(+) DC in HIV(+) patients were associated with high CD3(+)CD4(-)DR(+) lymphocyte counts, IL-10(+) DC were associated with the proportion of IgG-coated CD4(+) blood lymphocytes. These data suggest that long-term HIV-infected hemophilia patients with clinically stable disease have normal levels of functional IL-10(+) DC and IL-12(+) DC that might be involved in halting the progression of disease.
Daniel
Volker
V
Institute of Immunology, Department of Transplantation Immunology, University of Heidelberg, Heidelberg, Germany. Volker.Daniel@med.uni-heidelberg.de
Naujokat
Cord
C
Sadeghi
Mahmoud
M
Zimmermann
Rainer
R
Huth-Kühne
Angela
A
Opelz
Gerhard
G
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D018450:Disease Progression; D005260:Female; D015699:HIV Envelope Protein gp120 / Q000276:immunology / Q000378:metabolism; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D020276:HIV Long-Term Survivors; D015497:HIV-1; D006467:Hemophilia A / Q000150:complications / Q000276:immunology* / Q000821:virology; D006801:Humans; D018664:Interleukin-12 / Q000032:analysis* / Q000276:immunology; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D019798:Neopterin / Q000097:blood; D012367:RNA, Viral / Q000097:blood
D015699:HIV Envelope Protein gp120; D012367:RNA, Viral; D018664:Interleukin-12; D019798:Neopterin