Humanized NOD/SCID/IL2Rgamma(null) mice transplanted with hematopoietic stem cells under nonmyeloablative conditions show prolonged life spans and allow detailed analysis of human immunodeficiency virus type 1 pathogenesis.
In a previous study, we demonstrated that humanized NOD/SCID/IL2Rgamma(null) (hNOG) mice constructed with human hematopoietic stem cells (HSCs) allow efficient human immunodeficiency virus type 1 (HIV-1) infection. However, HIV-1 infection could be monitored for only 43 days in the animals due to their short life spans. By transplanting HSCs without any myeloablation methods, the mice successfully survived longer than 300 days with stable engraftment of human cells. The mice showed high viremia state for more than the 3 months examined, with systemic HIV-1 infection and gradual decrease of CD4+ T cells analogous to that in humans. These capacities of the hNOG mice are very attractive for modeling mechanisms of AIDS progression and therapeutic strategy.
Watanabe
Satoru
S
Department of Virology, Division of Medical Science, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
Ohta
Shinrai
S
Yajima
Misako
M
Terashima
Kazuo
K
Ito
Mamoru
M
Mugishima
Hideo
H
Fujiwara
Shigeyoshi
S
Shimizu
Kazufumi
K
Honda
Mitsuo
M
Shimizu
Norio
N
Yamamoto
Naoki
N
D000818:Animals; D018791:CD4 Lymphocyte Count; D004195:Disease Models, Animal; D005260:Female; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000254:growth & development*; D018380:Hematopoietic Stem Cell Transplantation; D051379:Mice; D018345:Mice, Knockout; D016513:Mice, SCID; D014766:Viremia