Identification and characterization of zebrafish semaphorin 6D.
The semaphorin gene family contains a large number of secreted and transmembrane proteins; some function as repulsive and attractive cues of axon guidance during development. Here, we report cloning and characterization of zebrafish transmembrane semaphorin gene, semaphorin 6D (sema6D). Sema6D is expressed predominantly in the nervous system during embryogenesis, as determined by in situ hybridization. We also found that Sema6D binds Plexin-A1 in vitro, but not other Plexins. It induces the repulsion of dorsal root ganglion axons, but not sympathetic axons. Consequently, Sema6D might use Plexin-A1 as a receptor to repel specific types of axons during development.
Kimura
Masafumi
M
Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Taniguchi
Masahiko
M
Mikami
Yoshinori
Y
Masuda
Tomoyuki
T
Yoshida
Tomoyuki
T
Mishina
Masayoshi
M
Shimizu
Takao
T
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D005727:Ganglia, Spinal / Q000196:embryology / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D039961:Semaphorins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D020033:Protein Isoforms; D011956:Receptors, Cell Surface; D039961:Semaphorins; D029961:Zebrafish Proteins; C526275:semaphorin 6D, zebrafish