Molecular phylogeny of the pinworms of mice, rats and rabbits, and its use to develop molecular beacon assays for the detection of pinworms in mice.
Though pinworm infestation has been prevalent since the early years of laboratory animal medicine, the genomes of these parasites have not yet been sequenced. The authors used high-fidelity polymerase chain reaction to amplify a large portion of the ribosomal gene complex of four pinworm species commonly found in lab rodents and rabbits (Aspiculuris tetraptera, Passalurus ambiguus, Syphacia muris and Syphacia obvelata). They determined DNA sequences for these complexes and carried out phylogenetic analysis. Using this information, the authors developed real-time molecular beacon assays for pinworm detection, comparing the new diagnostic approach with traditional methods such as perianal tape testing, fecal flotation and direct examination of intestinal content.
Feldman
Sanford H
SH
Center for Comparative Medicine, University of Virginia, Charlottesville, VA 22908, USA. shf2b@virginia.edu
Bowman
Susan G
SG
D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics; D017229:Enterobiasis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D004757:Enterobius / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011817:Rabbits; D051381:Rats; D012376:Rodent Diseases / Q000175:diagnosis / Q000469:parasitology*; D017422:Sequence Analysis, DNA
D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV