title: Complete nucleotide sequence of pLD-TEX-KL, a 66-kb plasmid of Legionella dumoffii TEX-KL strain.
abstract: The complete nucleotide sequence of a large (66 kb) plasmid pLD-TEX-KL of Legionella dumoffii TEX-KL strain was determined. Of the 57 predicted open reading frames (ORFs), 39 (68%) encoded proteins similar to previously known proteins, five (9%) were assigned with putative functions, three (5%) encoded conserved hypothetical proteins, and 10 (18%) had no homology to any genes present in the current open databases. The ORFs with similar functions were organized in a modular structure; thus, transfer region was identified, as well as a putative heavy-metal ion transporter system (hel). The transfer region encoded homologs of the Salmonella entrica serovar Typhi conjugative system components involved in conjugation. In addition, we also found a potential protein that was analogous to the DNA polymerase III epsilon subunit. It is rarely found that plasmid encode the DNA polymerase.
authors: Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan. qtian@bact.med.kyushu-u.ac.jp
authors: Tian
authors: T
authors: Qin
authors: Hideki
authors: H
authors: Hirakawa
authors: Ken-ichiro
authors: K
authors: Iida
authors: Kenshiro
authors: K
authors: Oshima
authors: Masahira
authors: M
authors: Hattori
authors: Kosuke
authors: K
authors: Tashiro
authors: Satoru
authors: S
authors: Kuhara
authors: Shin-ichi
authors: S
authors: Yoshida
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D003227:Conjugation, Genetic / Q000235:genetics; D004258:DNA Polymerase III / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D007875:Legionella / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010957:Plasmids / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D004258:DNA Polymerase III