[Application of MiniFiler kit in forensic DNA testing of low copy number template].
OBJECTIVE
To detect low copy number of DNA samples by using a newly launched commercial miniSTR detection kit (MiniFiler) in forensic practice.


METHODS
Low concentration and/or challenged forensic DNA samples were analyzed according to protocols provided by the manufacturer (Applied Biosystems, Foster City, USA).


RESULTS
DNA samples as low as 10 pg could be amplified by MiniFiler kit, and the optimal DNA quantity was 40 pg or above.


CONCLUSIONS
MiniFiler kit can be used for analysis of low copy number STR.
Tang
Jian-ping
JP
Institute of Forensic Science, Shanghai Municipal Public Security Bureau, Shanghai 200083, China.
Wu
Dan
D
Zhang
Chen
C
Zhou
Huai-gu
HG
D000483:Alleles; D016172:DNA Fingerprinting / Q000295:instrumentation* / Q000379:methods; D053803:Forensic Genetics / Q000379:methods*; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D020080:Tandem Repeat Sequences; D013698:Templates, Genetic
D011933:Reagent Kits, Diagnostic