Chemical cross-linking and mass spectrometry as structure determination tools.
Chemical cross-linking is becoming a valuable tool for the high-order structure determination of proteins and protein complexes. Cross-linking methodology is able to provide low-resolution structures when at least something is known already about the proteins under investigation. The suitability of top-down and bottom-up methodologies is discussed and further potential applications of chemical cross-linking of proteins, as well as combinations with other techniques such as hydrogen/deuterium exchange and molecular modeling, are suggested.
Novak
Petr
P
Institute of Microbiology, Academy of Sciences, Prague, Czech Republic.
Giannakopulos
Anastassios E
AE
D000818:Animals; D003432:Cross-Linking Reagents / Q000737:chemistry*; D006801:Humans; D011487:Protein Conformation; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D003432:Cross-Linking Reagents; D011506:Proteins