Validation of a PCR-oligochromatography test for detection of Trypanozoon parasites in a multicenter collaborative trial.
A PCR-oligochromatography test for diagnosis of human and animal trypanosomiasis was evaluated through a multicenter ring trial with six laboratories testing a set of 21 blinded samples, resulting in qualitative data (positive or negative). Results showed an intralaboratory repeatability (accordance) of 88.7% (credible interval [CI], 84.4 to 92.5%) and an interlaboratory repeatability (concordance) of 88.1% (CI, 84.3 to 92.3%).
Claes
F
F
Faculty of Bioscience Engineering, K U Leuven, Department of Biosystems, Kasteelpark Arenberg 30, 3001, Leuven, Belgium. filip.claes@biw.kuleuven.be
Deborggraeve
S
S
Verloo
D
D
Mertens
P
P
Crowther
J R
JR
Leclipteux
T
T
Büscher
P
P
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D014345:Trypanosoma / Q000235:genetics / Q000302:isolation & purification*
D016054:DNA, Protozoan