Metabolic engineering of Corynebacterium glutamicum for cadaverine fermentation.
Cadaverine, the expected raw material of polyamides, is produced by decarboxylation of L-lysine. If we could produce cadaverine from the cheapest sugar, and as a renewable resource, it would be an effective solution against global warming, but there has been no attempt to produce cadaverine from glucose by fermentation. We focused on Corynebacterium glutamicum, whose L-lysine fermentation ability is superior, and constructed a metabolically engineered C. glutamicum in which the L-homoserine dehydrogenase gene (hom) was replaced by the L-lysine decarboxylase gene (cadA) of Escherichia coli. In this recombinant strain, cadaverine was produced at a concentration of 2.6 g/l, equivalent to up to 9.1% (molecular yield) of the glucose transformed into cadaverine in neutralizing cultivation. This is the first report of cadaverine fermentation by C. glutamicum.
Mimitsuka
Takashi
T
New Frontiers Research Laboratories, Toray Industries, Inc., 6-10-1 Tebiro, Kamakura 248-8555, Japan. Takashi_Mimitsuka@nts.toray.co.jp
Sawai
Hideki
H
Hatsu
Masahiro
M
Yamada
Katsushige
K
D002103:Cadaverine / Q000737:chemistry / Q000378:metabolism*; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering; D008239:Lysine / Q000378:metabolism; D015394:Molecular Structure; D016366:Open Reading Frames / Q000235:genetics
D008239:Lysine; D002103:Cadaverine