title: Structural effects on the biodistribution and positron emission tomography (PET) imaging of well-defined (64)Cu-labeled nanoparticles comprised of amphiphilic block graft copolymers.
abstract: The synthesis of poly(methyl methacrylate-co-methacryloxysuccinimide-graft-poly(ethylene glycol)) (PMMA-co-PMASI-g-PEG) via living free radical polymerization provides a convenient route to well-defined amphiphilic graft copolymers having a controllable number of reactive functional groups, variable length PEG grafts, and low polydispersity. These copolymers were shown to form PMMA-core/PEG-shell nanoparticles upon hydrophobic collapse in water, with the hydrodynamic size being defined by the molecular weight of the backbone and the PEG grafts. Functionalization of these polymeric nanoparticles with a 1,4,7,10-tetraazacyclododecanetetraacetic acid (DOTA) ligand capable of chelating radioactive 64Cu nuclei enabled the biodistribution and in vivo positron emission tomography of these materials to be studied and directly correlated to the initial structure. Results indicate that nanoparticles with increasing PEG chain lengths show increased blood circulation and low accumulation in excretory organs, suggesting the possible use of these materials as stealth carriers for medical imaging and systemic administration.
authors: Materials Research Laboratory, Department of Chemistry, University of California, Santa Barbara, CA 93106, USA.
authors: Eric D
authors: ED
authors: Pressly
authors: Raffaella
authors: R
authors: Rossin
authors: Aviv
authors: A
authors: Hagooly
authors: Ken-Ichi
authors: K
authors: Fukukawa
authors: Benjamin W
authors: BW
authors: Messmore
authors: Michael J
authors: MJ
authors: Welch
authors: Karen L
authors: KL
authors: Wooley
authors: Matthew S
authors: MS
authors: Lamm
authors: Rohan A
authors: RA
authors: Hule
authors: Darrin J
authors: DJ
authors: Pochan
authors: Craig J
authors: CJ
authors: Hawker
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D003301:Copper Radioisotopes / Q000737:chemistry / Q000493:pharmacokinetics*; D020285:Cryoelectron Microscopy; D005260:Female; D005609:Free Radicals; D008024:Ligands; D008689:Methacrylates / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry; D049268:Positron-Emission Tomography / Q000295:instrumentation* / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001672:Biocompatible Materials; D003301:Copper Radioisotopes; D005609:Free Radicals; D008024:Ligands; D008689:Methacrylates; C421283:poly(ethylene glycol)-dimethacrylate; D011092:Polyethylene Glycols; D003300:Copper; D019904:Polymethyl Methacrylate