Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants.
To improve the accumulation of recombinant human epidermal growth factor (hEGF) in transgenic tobacco, a highly effective vector was constructed and transformed via Agrobacterium tumefaciens. The hEGF content in transgenic tobacco was up to 0.3% of the total soluble protein. Using the Vero E6 cell expansion assay and the MTT method for cell proliferation, hEGF produced by transgenic tobacco significantly stimulated Vero E6 cell expansion and proliferation, the same as commercial hEGF products.
Bai
Jie-Ying
JY
The National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, 100871, PR China.
Zeng
Lin
L
Hu
Yuan-Lei
YL
Li
Yan-Fang
YF
Lin
Zhong-Ping
ZP
Shang
Shi-Chen
SC
Shi
Yan-Sheng
YS
D000818:Animals; D015152:Blotting, Northern; D049109:Cell Proliferation / Q000187:drug effects; D016009:Chi-Square Distribution; D002522:Chlorocebus aethiops; D003062:Codon; D004815:Epidermal Growth Factor / Q000378:metabolism* / Q000494:pharmacology; D005822:Genetic Vectors; D006801:Humans; D008934:Mitogens / Q000494:pharmacology; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics*; D014709:Vero Cells
D003062:Codon; D008934:Mitogens; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor