Bivalent peptides as PDZ domain ligands.
A series of multivalent peptides, with the ability to simultaneously bind two separate PDZ domain proteins, has been designed, synthesized, and tested by isothermal titration calorimetry (ITC). The monomer sequences, linked with succinate, varied in length from five to nine residues. The thermodynamic binding parameters, in conjunction with results from mass spectrometry, indicate that a ternary complex is formed in which each peptide arm binds two equivalents of the third PDZ domain (PDZ3) of the neuronal protein PSD-95.
Klosi
Edvin
E
Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
Saro
Dorina
D
Spaller
Mark R
MR
D000595:Amino Acid Sequence; D008024:Ligands; D008969:Molecular Sequence Data; D054731:PDZ Domains; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D013816:Thermodynamics
D008024:Ligands; D010455:Peptides