title: Lack of association between vitamin D receptor genotypes and haplotypes with fat-free mass in postmenopausal Brazilian women.
abstract: The relationship between vitamin D receptor (VDR) ApaI, CDX2, BsmI, FokI, and TaqI polymorphisms and fat-free mass (FFM) were examined in 191 postmenopausal Brazilian women (mean age 67.87 +/- 5.22 years). Participants underwent FFM measurements by dual-energy x-ray absorptiometry (DEXA). Whole-blood-extracted genomic DNA was genotyped to the aforementioned polymorphisms and to ancestry-informative markers through minisequencing, using the SNaPshot Multiplex System. Association between VDR polymorphisms and FFM variables was assessed by analysis of covariance. Haplotypes were estimated, and regression-based, haplotype-specific association tests were carried out with the studied phenotypes. No departure from Hardy-Weinberg equilibrium was detected for any polymorphism. None of the investigated VDR allelic variations, individually or analyzed as haplotypes, was associated with FFM phenotypes. The inclusion of individual African genomic ancestry was used as an attempt to correct for population stratification. Further studies in larger sample population are required to confirm these findings.
authors: Programa de Pós-Graduação em Educação Física da Universidade Católica de Brasília, Brazil.
authors: Ricardo
authors: R
authors: Moreno Lima
authors: Breno
authors: B
authors: Silva de Abreu
authors: Paulo
authors: P
authors: Gentil
authors: Tulio
authors: T
authors: Cesar de Lima Lins
authors: Dário
authors: D
authors: Grattapaglia
authors: Rinaldo Wellerson
authors: RW
authors: Pereira
authors: Ricardo
authors: R
authors: Jacó de Oliveira
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001483:Base Sequence; D001823:Body Composition / Q000235:genetics*; D001938:Brazil; D003430:Cross-Sectional Studies; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008593:Menopause; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D018167:Receptors, Calcitriol / Q000235:genetics*
chemical_list: D018167:Receptors, Calcitriol