title: Role of neuronal nitric oxide in the regulation of vasopressin expression and release in response to inhibition of catecholamine synthesis and dehydration.
abstract: We used neuronal nitric oxide synthase (nNOS) gene knockout mice to study the effects of catecholamines and neuronal nitric oxide on vasopressin expression in the hypothalamic neurosecretory centers. nNOS gene deletion did not change the level of vasopressin mRNA in the supraoptic or paraventricular nuclei. In contrast, vasopressin immunoreactivity was lower in nNOS deficient mice than in wild-type animals. Dehydration increased vasopressin mRNA levels and decreased vasopressin immunoreactivity in both wild-type and nNOS knockout mice, but these responses were more marked in the nNOS knockout mice. Treatment with alpha-mpt, a pharmacologic inhibitor of catecholamine synthesis, resulted in increased vasopressin mRNA levels in wild-type mice and in reduced vasopressin immunoreactivity in both wild-type and nNOS knockout mice. From these results, we conclude: (1) neuronal nitric oxide suppresses vasopressin expression under basal conditions and during activation of the vasopressinergic system by dehydration; (2) catecholamines limit vasopressin expression; (3) nNOS is required for the effects of catecholamines on vasopressin expression.
authors: Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, 44 Thorez pr, Saint-Petersburg 194223, Russia.
authors: Liubov
authors: L
authors: Yamova
authors: Dmitriy
authors: D
authors: Atochin
authors: Margarita
authors: M
authors: Glazova
authors: Elena
authors: E
authors: Chernigovskaya
authors: Paul
authors: P
authors: Huang
mesh_terms: D000818:Animals; D002395:Catecholamines / Q000096:biosynthesis*; D003681:Dehydration / Q000503:physiopathology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009569:Nitric Oxide / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000172:deficiency / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects; D014667:Vasopressins / Q000187:drug effects / Q000378:metabolism*; D019805:alpha-Methyltyrosine / Q000494:pharmacology
chemical_list: D002395:Catecholamines; D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D014667:Vasopressins; D009569:Nitric Oxide; D019805:alpha-Methyltyrosine; D052248:Nitric Oxide Synthase Type I