Antioxidant defence enzyme activities in hepatopancreas, gills and muscle of Spiny cheek crayfish (Orconectes limosus) from the River Danube.
The aim of our study was to determine the activity of antioxidant defence (AD) enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and the phase II biotransformation enzyme glutathione-S-transferase (GST) in the hepatopancreas, the gills and muscle of Spiny cheek crayfish (Orconectes limosus) from the River Danube and to compare tissue specificities of investigated enzymes. Our results indicated that both specific and total SOD activities in the hepatopancreas were lower compared to the gills and muscle. Total SOD activity in the gills was lower with respect to that in muscle. CAT and GSH-Px (both specific and total) activities were higher in the hepatopancreas compared to those in the gills and muscle. In the gills the specific and total GR activities were higher than in the hepatopancreas and muscle. The specific and total GST activities were higher in the hepatopancreas compared with the gills and muscle. Our study represents the first comprehensive report of AD enzymes in tissues of O. limosus caught in the River Danube. The noted tissue distributions of the investigated AD enzyme activities most likely reflected different metabolic activities and different responses to environmental conditions in the examined tissues.
Borković
Slavica S
SS
Department of Physiology, Institute for Biological Research Sinisa Stanković, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
Pavlović
Sladjan Z
SZ
Kovacević
Tijana B
TB
Stajn
Andras S
AS
Petrović
Vojislav M
VM
Saicić
Zorica S
ZS
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D003400:Astacoidea / Q000201:enzymology*; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods; D004798:Enzymes / Q000378:metabolism*; D005260:Female; D005618:Fresh Water; D005880:Gills / Q000201:enzymology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D043143:Hepatopancreas / Q000201:enzymology*; D008297:Male; D009132:Muscles / Q000201:enzymology*; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism; D014018:Tissue Distribution; D015022:Yugoslavia
D000975:Antioxidants; D015415:Biomarkers; D004798:Enzymes; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005982:Glutathione Transferase