Molecularly imprinted solid-phase extraction combined with electrochemical oxidation fluorimetry for the determination of methotrexate in human serum and urine.
The method of synthesis and evaluation of molecularly imprinted polymers was reported. As a selective solid-phase extraction sorbent, the polymers were coupled with electrochemical fluorimetry detection for the efficient determination of methotrexate in serum and urine. Methotrexate was preconcentrated in the molecularly imprinted solid-phase extraction microcolumn packed with molecularly imprinted polymers, and then eluted. The eluate was detected by fluorescence spectrophotometer after electrochemical oxidation. The conditions of preconcentration, elution, electrochemical oxidation and determination were carefully studied. Under the selected experimental conditions, the calibration graph of the fluorescence intensity versus methotrexate concentration was linear from 4x10(-9) g mL(-1) to 5x10(-7) g mL(-1), and the detection limit was 8.2x10(-10) g mL(-1) (3sigma). The relative standard deviation was 3.92% (n=7) for 1x10(-7) g mL(-1) methotrexate. The experiments showed that the selectivity and sensitivity of fluorimetry could be greatly improved by the proposed method. This method has been successfully applied to the determination of methotrexate. At the same time, the binding characteristics of the polymers to the methotrexate were evaluated by batch and dynamic methods.
Chen
Suming
S
Institute of Analytical Science, School of Chemistry and Chemical Engineering, University of Southwest, Chongqing 400715, PR China.
Zhang
Zhujun
Z
D000964:Antimetabolites, Antineoplastic / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D002138:Calibration; D004563:Electrochemistry; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D008727:Methotrexate / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D052616:Solid Phase Extraction; D013050:Spectrometry, Fluorescence
D000964:Antimetabolites, Antineoplastic; D008727:Methotrexate