title: Dynamic interactions of Ntr1-Ntr2 with Prp43 and with U5 govern the recruitment of Prp43 to mediate spliceosome disassembly.
abstract: The Saccharomyces cerevisiae splicing factors Ntr1 (also known as Spp382) and Ntr2 form a stable complex and can further associate with DExD/H-box RNA helicase Prp43 to form a functional complex, termed the NTR complex, which catalyzes spliceosome disassembly. We show that Prp43 interacts with Ntr1-Ntr2 in a dynamic manner. The Ntr1-Ntr2 complex can also bind to the spliceosome first, before recruiting Prp43 to catalyze disassembly. Binding of Ntr1-Ntr2 or Prp43 does not require ATP, but disassembly of the spliceosome requires hydrolysis of ATP. The NTR complex also dynamically interacts with U5 snRNP. Ntr2 interacts with U5 component Brr2 and is essential for both interactions of NTR with U5 and with the spliceosome. Ntr2 alone can also bind to U5 and to the spliceosome, suggesting a role of Ntr2 in mediating the binding of NTR to the spliceosome through its interaction with U5. Our results demonstrate that dynamic interactions of NTR with U5, through the interaction of Ntr2 with Brr2, and interactions of Ntr1 and Prp43 govern the recruitment of Prp43 to the spliceosome to mediate spliceosome disassembly.
authors: Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, Taiwan, Republic of China.
authors: Rong-Tzong
authors: RT
authors: Tsai
authors: Chi-Kang
authors: CK
authors: Tseng
authors: Pei-Jung
authors: PJ
authors: Lee
authors: Hsin-Chou
authors: HC
authors: Chen
authors: Ru-Huei
authors: RH
authors: Fu
authors: Kae-jiun
authors: KJ
authors: Chang
authors: Fu-Lung
authors: FL
authors: Yeh
authors: Soo-Chen
authors: SC
authors: Cheng
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000908:Antibodies, Fungal / Q000494:pharmacology; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D017415:Ribonucleoprotein, U5 Small Nuclear / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D017381:Spliceosomes / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000908:Antibodies, Fungal; C507653:Ntr2 protein, S cerevisiae; D017415:Ribonucleoprotein, U5 Small Nuclear; D029701:Saccharomyces cerevisiae Proteins; C507652:Spp382 protein, S cerevisiae; D000255:Adenosine Triphosphate; C109034:PRP43 protein, S cerevisiae; D053487:DEAD-box RNA Helicases