Isolation of microRNA targets using biotinylated synthetic microRNAs.
MicroRNAs are small regulatory RNAs found in multicellular organisms where they post-transcriptionally regulate gene expression. In animals, microRNAs bind mRNAs via incomplete base pairings making the identification of microRNA targets inherently difficult. Here, we present a detailed method for experimental identification of microRNA targets based on affinity purification of tagged microRNAs associated with their targets.
Orom
Ulf Andersson
UA
Biotech Research & Innovation Centre, Ole Maaløes Vej 5, 2200 Copenhagen, Denmark.
Lund
Anders H
AH
D000818:Animals; D001483:Base Sequence; D001710:Biotin; D002460:Cell Line; D002846:Chromatography, Affinity / Q000379:methods; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics; D017344:Genes, Insect; D006801:Humans; D035683:MicroRNAs / Q000138:chemical synthesis / Q000235:genetics* / Q000302:isolation & purification*; D009479:Neuropeptides / Q000235:genetics; D014162:Transfection
D029721:Drosophila Proteins; C108880:HID protein, Drosophila; D035683:MicroRNAs; D009479:Neuropeptides; C471146:bantam microRNA, Drosophila; D001710:Biotin