NNAT and DIRAS3 genes are paternally expressed in pigs.
Although expression and epigenetic differences of imprinted genes have been extensively characterised in man and the mouse, little is known on livestock species. In this study, the polymorphism-based approach was used to detect the imprinting status of NNAT and DIRAS3 genes in five heterozygous pigs (based on SNP) of Large White and Meishan F(1) hybrids. The results show that both genes were paternally expressed in all the tested tissues (heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus, ovary and pituitary). In addition, the NNAT gene had two transcripts in all tested tissues, which is consistent with its counterpart in man and cattle.
Cheng
Huan-Chen
HC
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agriculture University, Wuhan, P. R. China.
Zhang
Feng-Wei
FW
Deng
Chang-Yan
CY
Jiang
Cao-De
CD
Xiong
Yuan-Zhu
YZ
Li
Feng-E
FE
Lei
Ming-Gang
MG
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005260:Female; D015870:Gene Expression; D018392:Genomic Imprinting; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D013045:Species Specificity; D034421:Sus scrofa / Q000235:genetics*; D014018:Tissue Distribution; D020741:rho GTP-Binding Proteins / Q000235:genetics*
D017931:DNA Primers; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D004247:DNA; D020741:rho GTP-Binding Proteins