title: Functional genomics of the chicken--a model organism.
abstract: Since the sequencing of the genome and the development of high-throughput tools for the exploration of functional elements of the genome, the chicken has reached model organism status. Functional genomics focuses on understanding the function and regulation of genes and gene products on a global or genome-wide scale. Systems biology attempts to integrate functional information derived from multiple high-content data sets into a holistic view of all biological processes within a cell or organism. Generation of a large collection ( approximately 600K) of chicken expressed sequence tags, representing most tissues and developmental stages, has enabled the construction of high-density microarrays for transcriptional profiling. Comprehensive analysis of this large expressed sequence tag collection and a set of approximately 20K full-length cDNA sequences indicate that the transcriptome of the chicken represents approximately 20,000 genes. Furthermore, comparative analyses of these sequences have facilitated functional annotation of the genome and the creation of several bioinformatic resources for the chicken. Recently, about 20 papers have been published on transcriptional profiling with DNA microarrays in chicken tissues under various conditions. Proteomics is another powerful high-throughput tool currently used for examining the dynamics of protein expression in chicken tissues and fluids. Computational analyses of the chicken genome are providing new insight into the evolution of gene families in birds and other organisms. Abundant functional genomic resources now support large-scale analyses in the chicken and will facilitate identification of transcriptional mechanisms, gene networks, and metabolic or regulatory pathways that will ultimately determine the phenotype of the bird. New technologies such as marker-assisted selection, transgenics, and RNA interference offer the opportunity to modify the phenotype of the chicken to fit defined production goals. This review focuses on functional genomics in the chicken and provides a road map for large-scale exploration of the chicken genome.
authors: Department of Animal and Food Sciences, University of Delaware, Newark 19717, USA. cogburn@udel.edu
authors: L A
authors: LA
authors: Cogburn
authors: T E
authors: TE
authors: Porter
authors: M J
authors: MJ
authors: Duclos
authors: J
authors: J
authors: Simon
authors: S C
authors: SC
authors: Burgess
authors: J J
authors: JJ
authors: Zhu
authors: H H
authors: HH
authors: Cheng
authors: J B
authors: JB
authors: Dodgson
authors: J
authors: J
authors: Burnside
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics*; D005786:Gene Expression Regulation; D023281:Genomics; D023421:Models, Animal