Release of soluble vascular endothelial growth factor receptor-1 (sFlt-1) during coronary artery bypass surgery.
BACKGROUND
This study was conducted to follow plasma concentrations of sFlt-1 and sKDR, two soluble forms of the vascular endothelial growth factor (VEGF) receptor in patients undergoing coronary artery bypass graft (CABG) surgery with extracorporeal circulation (ECC).


METHODS
Plasma samples were obtained before, during and after surgery in 15 patients scheduled to undergo CABG. Levels of sFlt-1 and KDR levels were investigated using specific ELISA.


RESULTS
A 75-fold increase of sFlt-1 was found during cardiac surgery, sFlt-1 levels returning to pre-operative values at the 6th post-operative hour. In contrast sKDR levels did not change during surgery. The ECC-derived sFlt-1 was functional as judge by its inhibitory effect on the VEGF mitogenic response in human umbilical vein endothelial cells (HUVECs). Kinetic experiments revealed sFlt-1 release immediately after the beginning of ECC suggesting a proteolysis of its membrane form (mFlt-1) rather than an elevated transcription/translation process. Flow cytometry analysis highlighted no effect of ECC on the shedding of mFlt-1 on platelets and leukocytes suggesting vascular endothelial cell as a putative cell source for the ECC-derived sFlt-1.


CONCLUSIONS
sFlt-1 is released during CABG with ECC. It might be suggested that sFlt-1 production, by neutralizing VEGF and/or by inactivating membrane-bound Flt-1 and KDR receptors, might play a role in the occurrence of post-CABG complication.
Denizot
Yves
Y
UMR CNRS 6101, Centre National de Recherche Scientifique, Université de Limoges, France. yves.denizot@unilim.fr
Leguyader
Alexandre
A
Cornu
Elisabeth
E
Laskar
Marc
M
Orsel
Isabelle
I
Vincent
Christelle
C
Nathan
Nathalie
N
D000222:Adaptation, Physiological; D001685:Biological Factors / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism; D001026:Coronary Artery Bypass; D004730:Endothelium, Vascular / Q000378:metabolism; D005112:Extracorporeal Circulation; D005434:Flow Cytometry; D006801:Humans; D007700:Kinetics; D007962:Leukocytes / Q000378:metabolism; D016343:Monitoring, Intraoperative; D011184:Postoperative Period; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000032:analysis / Q000097:blood*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000097:blood
D001685:Biological Factors; D042461:Vascular Endothelial Growth Factor A; D040281:Vascular Endothelial Growth Factor Receptor-1; D040301:Vascular Endothelial Growth Factor Receptor-2