Low- to high-throughput analysis of telomerase modulators with Telospot.
We designed a method termed Telospot to discover and characterize telomerase modulators as anticancer drugs or chemical biology tools. Telospot is based on a highly efficient human telomerase expression system and the detection of telomerase DNA reaction products in macroarray format. Telospot offers a highly scalable, cost- and time-effective alternative to presently available telomerase assays, which are limited by the requirement for PCR, telomerase purification or technologies not amenable to high throughput.
Cristofari
Gaël
G
Swiss Institute for Experimental Cancer Research, Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland.
Reichenbach
Patrick
P
Regamey
Pierre-Olivier
PO
Banfi
Damiano
D
Chambon
Marc
M
Turcatti
Gerardo
G
Lingner
Joachim
J
D000970:Antineoplastic Agents / Q000032:analysis*; D006801:Humans; D019098:Telomerase / Q000032:analysis / Q000378:metabolism*
D000970:Antineoplastic Agents; C509186:TERT protein, human; D019098:Telomerase