Respiratory disease markers in porcine bronchoalveolar lavage fluid.
In bronchoalveolar lavage fluid (BALF) of pigs originating from different herds bacteria, cells and the antibacterial peptide PR-39 were examined to gain information about the lung health status. In a high health nucleus herd 56% and in low health herds 20-100% of the examined pigs were found positive for potentially pathogenic bacteria. Based on these findings, a novel definition for bacterial respiratory tract disease was established using an 8% cut-off for the relative number of neutrophils in bronchoscopic and a 40% cut-off in transtracheal BALF in combination with the occurrence of potentially pathogenic microorganisms. The antibacterial peptide PR-39 was highly correlated to this definition of respiratory disease. An assessment of the bacteriological respiratory health status appears to be possibly based on the determination of PR-39 concentrations in BALF using different cut-off values according to the lavage method (2.5 nM for bronchoscopic and 5 nM for transtracheal BALF).
Hennig-Pauka
I
I
Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Hannover, Germany. isabel.hennig@tiho-hannover.de
Bremerich
S
S
Nienhoff
H
H
Schröder
C
C
Verspohl
J
J
Strutzberg-Minder
K
K
Ganter
M
M
Waldmann
K-H
KH
Blecha
F
F
Beyerbach
M
M
Gerlach
G-F
GF
D000818:Animals; D023181:Antimicrobial Cationic Peptides; D018893:Bronchoalveolar Lavage / Q000379:methods / Q000662:veterinary; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology* / Q000382:microbiology*; D001999:Bronchoscopy / Q000379:methods / Q000662:veterinary; D005260:Female; D007958:Leukocyte Count; D008297:Male; D009504:Neutrophils; D011237:Predictive Value of Tests; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000473:pathology
D023181:Antimicrobial Cationic Peptides; C072212:PR 39