title: Wounding and pathogen infection induce a chloroplast-targeted lipoxygenase in the common bean (Phaseolus vulgaris L.).
abstract: Chloroplastic LOXs are implicated in the biosynthesis of oxylipins like jasmonic acid and C6 volatiles among others. In this study, we isolated the cDNA of a novel chloroplast-targeted Phaseolus vulgaris LOX, (PvLOX6). This gene is highly induced after wounding, non-host pathogen infection, and by signaling molecules as H2O2, SA, ethylene and MeJA. The phylogenetic analysis of PvLOX6 showed that it is closely related to chloroplast-targeted LOX from potato (H1) and tomato (TomLOXC); both of them are implicated in the biosynthesis of C6 volatiles. Induction of PvLOX6 mRNA by wounding ethylene and jasmonic acid on the one side, and non-host pathogen, salicylic acid on the other indicates that common bean uses the same LOX to synthesize oxylipins in response to different stresses.
authors: Departmento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Cuernavaca, Morelos, C. P. 6100, Mexico. helena@ibt.unam.mx
authors: Helena
authors: H
authors: Porta
authors: Rosa Elia
authors: RE
authors: Figueroa-Balderas
authors: Mario
authors: M
authors: Rocha-Sosa
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002736:Chloroplasts / Q000378:metabolism*; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology; D004790:Enzyme Induction; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D008084:Lipoxygenase / Q000378:metabolism*; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000494:pharmacology; D027805:Phaseolus / Q000187:drug effects / Q000378:metabolism*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*
chemical_list: D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid; C036216:ethylene; D008084:Lipoxygenase