BAF53 interacts with p53 and functions in p53-mediated p21-gene transcription.
BAF53, a component of chromatin remodelling and histone acetyltransferase complexes, has been shown to be essential for cell survival in human cells and plays roles in p53-mediated gene transcription. However, the mechanism concerned in the process needs to be further explored. In this study, we show that BAF53 is involved in the repression of p53-dependent p21-gene transcription by interacting with p53 both in vivo and in vitro. Through electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation analyses, we demonstrate that BAF53 can reduce the p53-binding ability to p21 promoter. By western-blot experiments, we find that BAF53 can decrease p53-Lys382 acetylation, which may be partially responsible for the repression of p53-binding ability. Furthermore, BAF53 represses p21-promoter activity in a BRG1-independent manner. These data contribute to elucidating the molecular mechanisms of BAF53 in regulating p53-mediated gene transcription.
Wang
Min
M
Institute of Genetics and Cytology, Northeast Normal University, Changchun, China.
Gu
Chunsheng
C
Qi
Tianyang
T
Tang
Wen
W
Wang
Ling
L
Wang
Shuyan
S
Zeng
Xianlu
X
D000107:Acetylation; D000199:Actins / Q000378:metabolism*; D001665:Binding Sites; D015153:Blotting, Western; D002843:Chromatin / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D051548:Histone Acetyltransferases / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D011401:Promoter Regions, Genetic / Q000235:genetics; D014158:Transcription, Genetic / Q000502:physiology*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
C116160:ACTL6A protein, human; D000199:Actins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D050759:Cyclin-Dependent Kinase Inhibitor p21; D004268:DNA-Binding Proteins; D016159:Tumor Suppressor Protein p53; D051548:Histone Acetyltransferases