Eugenol modulates cyclooxygenase-2 expression through the activation of nuclear factor kappa B in human osteoblasts.
Because eugenol is a major component of root canal sealers and retrograde filling materials, its effects on periapical bone healing are therefore of concern. In this study, the effects of eugenol on the activation of nuclear factor kappa B (NF-kappaB) and the expression of cyclooxygenase-2 (COX-2) in human osteoblasts were investigated. The results showed that eugenol activated the nuclear translocation of NF-kappaB. In addition, COX-2 protein expression in osteoblasts was induced by eugenol in a dose-dependent manner. Furthermore, the eugenol-modulated COX-2 expression was inhibited by an NF-kappaB inhibitor, N-acetylcysteine. Taken together, eugenol might induce COX-2 expression through the activation of NF-kappaB in human osteoblasts. These results suggest that eugenol might be involved in periapical healing by impairing the functions of osteoblasts.
Lee
Ya-Yun
YY
Institute of Clinical Dentistry, National Yang-Ming University, Taipei, Taiwan.
Yang
Shue-Fen
SF
Ho
Weng-Hang
WH
Lee
Yuan-Ho
YH
Hung
Shan-Ling
SL
D000111:Acetylcysteine / Q000494:pharmacology; D015153:Blotting, Western; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000187:drug effects*; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D003593:Cytoplasm / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005054:Eugenol / Q000008:administration & dosage / Q000494:pharmacology*; D019085:Fluorescent Antibody Technique, Direct; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D018613:Microscopy, Confocal; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000187:drug effects*; D010006:Osteoblasts / Q000187:drug effects* / Q000201:enzymology; D012387:Root Canal Filling Materials / Q000494:pharmacology*
D052246:Cyclooxygenase 2 Inhibitors; D016166:Free Radical Scavengers; D016328:NF-kappa B; D012387:Root Canal Filling Materials; D005054:Eugenol; D051546:Cyclooxygenase 2; D000111:Acetylcysteine