Antiradical activity of Paulownia tomentosa (Scrophulariaceae) extracts.
Paulownia tomentosa is a large indecidous tree planted mostly for its fast growing wood and decorative purposes. The tree is also used in traditional Chinese medicine. As a part of our study of natural polyphenols, the fruits of Paulownia tomentosa were extracted by EtOH and than subjected to liquid/liquid extraction. Fractions were analysed by TLC and HPLC to determine presence of phenolic substances. We identified and quantified acteoside (1) and isoacteoside (2) in the EtOAc and n-BuOH extracts; mimulone (3) and diplacone (4) in the MeOH extract. To determine the antiradical activity of extracts we used the anti DPPH and peroxynitrite assays. The activity was expressed as Trolox C equivalents, IC50 for DPPH scavenging and a time dependency course was established. The polyphenols content was determined; results were expressed as gallic acid equivalents. Using these methods we found the fractions of the n-BuOH, EtOAc and MeOH extracts that display antiradical activity, which could be exploited as potential pharmaceuticals.
Smejkal
Karel
K
Natural Drugs Department, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1-3, CZ-612 42 Brno, Czech Republic. karel.mejkal@post.cz
Holubova
Pavla
P
Zima
Ales
A
Muselik
Jan
J
Dvorska
Margita
M
D004365:Drugs, Chinese Herbal / Q000302:isolation & purification* / Q000494:pharmacology; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000302:isolation & purification* / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry; D010636:Phenols / Q000302:isolation & purification* / Q000494:pharmacology; D010946:Plants, Medicinal; D059808:Polyphenols; D012997:Solvents; D014197:Trees
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D059808:Polyphenols; D012997:Solvents