[Initial study on discrimination of oral microorganisms with the metabonomics technique].
OBJECTIVE
To evaluate the feasibility of employing metabonomics method in identification of oral pathogenic bacteria.


METHODS
The Streptococcus mutans ATCC25175 and Actinomyces viscosus ATCC15987 were respectively inoculated in same certain culture medium. The growth curves of the inoculated bacteria were drown by turbidimetry. The culture solutions in four different growth phases of the both bacteria were used to test with the 1H-Nuclear magnetic resonance (1H-NMR) spectroscopy respectively. The data of 1H-NMR spectroscopy results were analyzed by principal components analysis (PCA).


RESULTS
The PCA showed the obvious clustering phenomena and the points of two group data stayed differentially together by two clusters. Therefore, the NMR-based metabonomics profiles can discriminate the two different kind of bacteria.


CONCLUSIONS
The metabonomics can be expected to be a kind of promising useful method in quick discrimination of oral pathogenic bacteria.
Li
Miao
M
Key. Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Xiao
Li-ying
LY
Li
Ji-yao
JY
Kong
Xiang-li
XL
Yu
Jia-hui
JH
Zhou
Jing-lin
JL
Xiao
Xiao-rong
XR
Zhu
Zhu
Z
Gong
Qi-mei
QM
Li
Wei
W
D003470:Culture Media; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics; D013295:Streptococcus mutans
D003470:Culture Media