The nucleoside and nucleotide mixture (OG-VI) rescues intestinal-like epithelial cells from the cytotoxicity of chemotherapeutic agents.
Immune cells and cells undergoing rapid turn-over can obtain exogenous nucleotides via salvage synthesis. We evaluated whether or not the balanced nucleoside and nucleotide mixture OG-VI, could rescue intestinal epithelial-like Caco-2 cells from the cytotoxic effects of several chemotherapeutic agents, in the presence and absence of glutamine (Gln). Cells were exposed to 5-fluorouracil (5FU), methotrexate (MTX) or 6-mercaptopurine (6MP), after which proliferation and cell cycle analyses were performed. Following exposure to the chemotherapeutic agents, we observed that cells treated with OG-VI proliferated well, whereas those without the supplement did not proliferate. Furthermore, following treatment with either 5FU or MTX, we observed that the number of cells in the G0/G1 phase decreased and those in the S phases increased. However, these cell cycle alterations were prevented by the addition of OG-VI. With the exception of 6MP-treated cells, we did not observe any effects on proliferation or cell cycle regulation that could be ascribed to the presence of Gln. Thus, we have demonstrated that OG-VI rescues cells from the cytotoxic effects of several chemotherapeutic agents.
Ono
Kaori
K
Department of Nutrition and Metabolism, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
Mawatari
Kazuaki
K
Harada
Nagakatsu
N
Takahashi
Akira
A
Sakai
Tohru
T
Ogoshi
Shohei
S
Nakaya
Yutaka
Y
D000970:Antineoplastic Agents / Q000633:toxicity*; D018938:Caco-2 Cells; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005472:Fluorouracil / Q000633:toxicity; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000187:drug effects*; D015122:Mercaptopurine / Q000633:toxicity; D008727:Methotrexate / Q000633:toxicity; D009705:Nucleosides / Q000494:pharmacology*; D009711:Nucleotides / Q000494:pharmacology*
D000970:Antineoplastic Agents; D009705:Nucleosides; D009711:Nucleotides; C087081:OG-VI solution; D015122:Mercaptopurine; D005472:Fluorouracil; D008727:Methotrexate