title: G and P rotavirus genotypes in stool samples from children in Teresina, State of Piauí.
abstract: A total of 123 stool specimens collected in Teresina, Piauí between 1994 and 1996, from 0 to 2-year-old children with diarrhea, were used for this study. Molecular characterization of the G and P rotavirus genotypes was performed using the reverse transcriptase polymerase chain reaction. The following results were obtained for the P genotypes: P[8] (17. 1%), P[1] (4. 9%), P[4] (3. 3%), P[6, M37] (2. 4%) and mixtures (27. 6%). The P[1]+P[8] mixture was found in 19. 5% of the samples. For the G genotypes, the results were: G1 (25. 2%), G5 (13. 8%), G2 (2. 5%), G4 (2. 5%), G9 (0. 8%) and mixtures (41. 5%). G1+G5 was the mixture most frequently found (12. 1%). Our results showed unusual combinations such as P[1]G5 and P[1]+P[8]G5. The high percentage of mixtures and unusual combinations containing mixtures of human and animal rotavirus genotypes strongly suggests the possibility of gene reassortment and interspecies transmission.
authors: Institute Pasteur São Paulo, SP Brasil. cimacedo@usp.br
authors: Carla Isabel
authors: CI
authors: Macedo
authors: Alice
authors: A
authors: Christofoletti
authors: Veridiana
authors: V
authors: Munford
authors: Maria Lúcia
authors: ML
authors: Rácz
mesh_terms: D001938:Brazil; D002675:Child, Preschool; D003968:Diarrhea, Infantile / Q000821:virology*; D005243:Feces / Q000821:virology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000821:virology*