title: Generation of functional multipotent adult stem cells from GPR125+ germline progenitors.
abstract: Adult mammalian testis is a source of pluripotent stem cells. However, the lack of specific surface markers has hampered identification and tracking of the unrecognized subset of germ cells that gives rise to multipotent cells. Although embryonic-like cells can be derived from adult testis cultures after only several weeks in vitro, it is not known whether adult self-renewing spermatogonia in long-term culture can generate such stem cells as well. Here, we show that highly proliferative adult spermatogonial progenitor cells (SPCs) can be efficiently obtained by cultivation on mitotically inactivated testicular feeders containing CD34+ stromal cells. SPCs exhibit testicular repopulating activity in vivo and maintain the ability in long-term culture to give rise to multipotent adult spermatogonial-derived stem cells (MASCs). Furthermore, both SPCs and MASCs express GPR125, an orphan adhesion-type G-protein-coupled receptor. In knock-in mice bearing a GPR125-beta-galactosidase (LacZ) fusion protein under control of the native Gpr125 promoter (GPR125-LacZ), expression in the testis was detected exclusively in spermatogonia and not in differentiated germ cells. Primary GPR125-LacZ SPC lines retained GPR125 expression, underwent clonal expansion, maintained the phenotype of germline stem cells, and reconstituted spermatogenesis in busulphan-treated mice. Long-term cultures of GPR125+ SPCs (GSPCs) also converted into GPR125+ MASC colonies. GPR125+ MASCs generated derivatives of the three germ layers and contributed to chimaeric embryos, with concomitant downregulation of GPR125 during differentiation into GPR125- cells. MASCs also differentiated into contractile cardiac tissue in vitro and formed functional blood vessels in vivo. Molecular bookmarking by GPR125 in the adult mouse and, ultimately, in the human testis could enrich for a population of SPCs for derivation of GPR125+ MASCs, which may be employed for genetic manipulation, tissue regeneration and revascularization of ischaemic organs.
authors: Howard Hughes Medical Institute, Department of Genetic Medicine, Weill Cornell Medical College, New York 10065, USA.
authors: Marco
authors: M
authors: Seandel
authors: Daylon
authors: D
authors: James
authors: Sergey V
authors: SV
authors: Shmelkov
authors: Ilaria
authors: I
authors: Falciatori
authors: Jiyeon
authors: J
authors: Kim
authors: Sai
authors: S
authors: Chavala
authors: Douglas S
authors: DS
authors: Scherr
authors: Fan
authors: F
authors: Zhang
authors: Richard
authors: R
authors: Torres
authors: Nicholas W
authors: NW
authors: Gale
authors: George D
authors: GD
authors: Yancopoulos
authors: Andrew
authors: A
authors: Murphy
authors: David M
authors: DM
authors: Valenzuela
authors: Robin M
authors: RM
authors: Hobbs
authors: Pier Paolo
authors: PP
authors: Pandolfi
authors: Shahin
authors: S
authors: Rafii
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000375:Aging; D000818:Animals; D001808:Blood Vessels / Q000166:cytology; D002066:Busulfan; D002454:Cell Differentiation; D002460:Cell Line; D020869:Gene Expression Profiling; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D009206:Myocardium / Q000166:cytology; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D012038:Regeneration; D013093:Spermatogonia / Q000166:cytology* / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000378:metabolism
chemical_list: C523437:GPR125 protein, mouse; D043562:Receptors, G-Protein-Coupled; D002066:Busulfan