The use of MTT [3-(4, 5-dimethyl-2-thiazolyl) -2, 5-diphenyl -2H- tetrazolium bromide]-reduction as an indicator of the effects of strain-specific, polyclonal rabbit antisera on Candida albicans and C. krusei.
There is only scanty data on the effects of specific antibody, with or without complement, on Candida albicans or Candida krusei in cell-free systems in vitro, although previously published work has shown that specific antibody mediates anti- Candida immunity in vivo by inhibition of adherence to host cells or surfaces and by the promotion of phagocytosis and intra-phagocytic killing. The MTT (3-[4, 5-dimethyl-2-thiazolyl] -2, 5-diphenyl -2H- tetrazolium bromide)-reduction method as a test of the viability of fungi was used to investigate the effect of complement, normal serum and immune serum on these two species of Candida that are of increasing importance as opportunistic pathogens. We report that normal rabbit serum or strain-specific, polyclonal anti- Candida rabbit antibody, with or without guinea pig complement, did not cause the reduction of total cell-mass or of the viability of either C. albicans or C. krusei, in vitro as determined by the MTT-reduction test. Complement alone without specific antibody, also, had no such effect on these two Candida species.
Arseculeratne
S N
SN
Department of Microbiology, Faculty of Veterinary Sciences, University of Peradeniya, Sri Lanka. chubby@sltnet.lk
Atapattu
D N
DN
Kumarasiri
R
R
Perera
D
D
Ekanayake
D
D
Rajapakse
J
J
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology*; D000918:Antibody Specificity; D002175:Candida / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology*; D003165:Complement System Proteins / Q000276:immunology; D006168:Guinea Pigs; D007106:Immune Sera / Q000737:chemistry / Q000276:immunology*; D010084:Oxidation-Reduction; D011817:Rabbits; D013045:Species Specificity; D013778:Tetrazolium Salts / Q000737:chemistry*; D013844:Thiazoles / Q000737:chemistry*
D000908:Antibodies, Fungal; D007106:Immune Sera; D013778:Tetrazolium Salts; D013844:Thiazoles; D003165:Complement System Proteins; C022616:thiazolyl blue