title: [Study on molecular cytogenetic abnormalities in multiple myeloma].
abstract: OBJECTIVE
  To explore the molecular cytogenetic abnormalities in multiple myeloma (MM).
  
  
  METHODS
  Bone marrow plasma cells from 23 previously untreated MM patients were purified by CD138 McAb magnetic cell sorting system, and a panel of probes for interphase fluorescence in situ hybridization were used to detect the 13q14 deletion, p53 deletion and IgH gene translocation in the sorted MM cells.
  
  
  RESULTS
  Among 23 MM patients, 13q14 deletion was observed in 10 (43.5%) cases, with the positive rate of 13q14 deleted cells ranged from 79% to 96%; 14q32 translocation was observed in 11 (47.8%) cases; 13q14 deletion and 14q32 translocation were simultaneously observed in 7 (30.4%) cases; and p53 deletion was observed in none of the 23 cases.
  
  
  CONCLUSIONS
  The frequency of 13q14 deletion and IgH gene translocation in multiple myeloma are high; and the relationship between 13q14 deletion, IgH gene translocation and prognosis is worth further investigating.
authors: Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, China.
authors: Shu-Yan
authors: SY
authors: Liu
authors: Jian-Yong
authors: JY
authors: Li
authors: Li-Juan
authors: LJ
authors: Chen
authors: Jin-Wen
authors: JW
authors: Huang
authors: Jin-Lan
authors: JL
authors: Pan
authors: Hai-Rong
authors: HR
authors: Qiu
authors: Yun-Feng
authors: YF
authors: Shen
authors: Wei
authors: W
authors: Xu
authors: Yong-Quan
authors: YQ
authors: Xue
mesh_terms: D000368:Aged; D002869:Chromosome Aberrations; D005260:Female; D017353:Gene Deletion; D015321:Gene Rearrangement; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics*; D010950:Plasma Cells
chemical_list: D007143:Immunoglobulin Heavy Chains