title: Up-regulation of platelet-derived growth factor by peripheral-blood leukocytes during experimental allergic encephalomyelitis.
abstract: In multiple sclerosis (MS) and its animal model, experimental allergic encephalomyelitis (EAE), clinical disease is associated with infiltration of the central nervous system (CNS) by immune cells. Subsequent remission with remyelination has been linked to an increased occurrence of oligodendrocyte progenitor (O2A) cells. Platelet-derived growth factor (PDGF) and fibroblast growth factor-2 (FGF-2) are key growth factors for O2A cells, yet little is known about their relevance in EAE and MS. We analyzed the expression of PDGF, FGF-2, and their receptors by peripheral-blood leukocytes (PBLs) and lymphocyte subsets during MBP-induced EAE. Strong up-regulation of PDGF, but not FGF-2, was observed in PBLs, with the highest expression after the disease maximum. T, NK, and NKT cells expressed PDGF, which is a novel observation because thus far only monocytes/macrophages have been reported to express PDGF. These results extend the idea that growth factors may contribute to improved CNS tissue repair, including PDGF, which is secreted by lesion-homing immune cells. The production of PDGF by lymphocytes may have potential therapeutic value when activating or modulating T-cell responses in demyelinating diseases.
authors: Department of Neurology, Hannover Medical School, Hannover, Germany. niklaskoehler@yahoo.com
authors: Niklas K U
authors: NK
authors: Koehler
authors: Markus
authors: M
authors: Roebbert
authors: Kourosh
authors: K
authors: Dehghani
authors: Matthias
authors: M
authors: Ballmaier
authors: Peter
authors: P
authors: Claus
authors: Stephan
authors: S
authors: von Hoersten
authors: Mona
authors: M
authors: Shing
authors: Per
authors: P
authors: Odin
authors: Jürgen
authors: J
authors: Strehlau
authors: Fedor
authors: F
authors: Heidenreich
mesh_terms: D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D007150:Immunohistochemistry; D007694:Killer Cells, Natural / Q000378:metabolism; D007962:Leukocytes / Q000276:immunology* / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011917:Rats, Inbred Lew; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2