Two formulations of the agricultural pesticide adjuvant, Toximul, reduce the glycogen content of HepG2 cells.
Young mice exposed dermally to the Toximul (Tox) class of agricultural pesticide adjuvants have reduced levels of hepatic glycogen, a marker of subclinical toxicity. The present study determined whether these effects on glycogen also occurred in cultured HepG2 cells. Exposure (3 hr) to Tox resulted in significant, concentration-dependent glycogen reductions (up to 70%) relative to control values (76 +/- 3 microg glycogen/mg protein). These reductions did not appear to be due to loss of cell viability, and were reversible with Tox removal. Two different formulations of Tox (3409F and MP-A) differed significantly in the magnitudes of glycogen reduction in the HepG2 cells.
Al-Khalidi
Mustafa
M
Department of Physiology, Dalhousie University, Halifax, Nova Scotia, Canada.
Acott
Philip D
PD
Crocker
John F S
JF
Geldenhuys
Laurette
L
O'Regan
Patrick A
PA
Upham
Jacqueline
J
Murphy
Mary G
MG
D000818:Animals; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical; D006003:Glycogen / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009930:Organic Chemicals / Q000633:toxicity; D010575:Pesticides / Q000633:toxicity*; D013501:Surface-Active Agents / Q000633:toxicity*
D009930:Organic Chemicals; D010575:Pesticides; D013501:Surface-Active Agents; C019116:Toximul MP8; D006003:Glycogen