title: Molecular classification of nodal metastasis in primary larynx squamous cell carcinoma.
abstract: Classification and prognosis of larynx squamous cell carcinoma (LSCC) depends on clinical and histopathological examination. Currently, expression profiling harbors the potential to investigate, classify, and better manage cancer. Gene expression profiles of 22 primary LSCCs were analyzed by microarrays containing 19,200 cDNAs. GOAL functionally classified differentially expressed genes, and a novel "in silico" procedure identified physical gene clusters differentially transcribed. A signature of 158 genes differentiated tumors with nodal metastasis. A novel statistical method allowed categorization of metastatic tumors into 2 distinct subgroups of differential gene expression patterns. Among genes correlated to nodal metastatic progression, we verified in vitro that NM23-H3 reduced cell motility and TRIM8 were a growth suppressor. Six chromosomal regions were specifically downregulated in metastatic tumors. This large-scale gene expression analysis in LSCC provides information on changes in genomic activity associated with lymphonodal metastasis and identifies molecules that might prove useful as novel therapeutic targets.
authors: Department of Maxillofacial Surgery, University of Ferrara, Ferrara, Italy.
authors: Francesco
authors: F
authors: Carinci
authors: Diego
authors: D
authors: Arcelli
authors: Lorenzo
authors: L
authors: Lo Muzio
authors: Francesca
authors: F
authors: Francioso
authors: Davide
authors: D
authors: Valentini
authors: Rita
authors: R
authors: Evangelisti
authors: Stefano
authors: S
authors: Volinia
authors: Anna
authors: A
authors: D'Angelo
authors: Germana
authors: G
authors: Meroni
authors: Massimo
authors: M
authors: Zollo
authors: Antonio
authors: A
authors: Pastore
authors: Franco
authors: F
authors: Ionna
authors: Filiberto
authors: F
authors: Mastrangelo
authors: Pio
authors: P
authors: Conti
authors: Stefano
authors: S
authors: Tetè
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000556:secondary*; D002352:Carrier Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D018076:DNA, Complementary / Q000235:genetics; D018450:Disease Progression; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics*; D009367:Neoplasm Staging; D009419:Nerve Tissue Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011379:Prognosis; D012334:RNA, Neoplasm / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D018076:DNA, Complementary; D054778:NM23 Nucleoside Diphosphate Kinases; D009419:Nerve Tissue Proteins; D012334:RNA, Neoplasm; C418604:TRIM8 protein, human; D025521:Tumor Suppressor Proteins; C517938:NME1 protein, human