Simultaneous quantitation of five flavonoid glycosides in Herba Epimedii by high-performance liquid chromatography-tandem mass spectrometry.
A rapid and accurate reversed-phase liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method has been developed and validated for the quantitative determination of five flavonoid glycosides, icariin, epimedin A, epimedin B, epimedin C and hyperin in Herba Epimedii. Chromatographic separations were performed using a C(18) narrow-bore HPLC column; a mixture of an aqueous solution of ammonium formate (pH 4.0) and acetonitrile was used as the mobile phase, with compounds detected in the positive ion mode with multiple-reaction monitoring using a triple-quadrupole mass spectrometer equipped with an electrospray ionisation interface. This method for the determination of the reported flavonoid glycosides was accurate and reproducible, with a lower limit of quantication of 0.5 microg/mL. The standard calibration curves for the above-mentioned compounds were linear (r(2) > 0.998) over the concentration range 0.5-10.0 microg/mL. The relative standard deviations for intra- and inter-day precision over the concentration range for the flavonoid glycosides were lower than 7.8% with accuracy between 90.1 and 111.0%. The established method was successfully applied to the quality assessment of samples of Herba Epimedii collected from Korea and China.
Islam
Nurul M
NM
Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, Seoul 130-650, Korea.
Yoo
Hye Hyun
HH
Lee
Min Woo
MW
Dong
Mi-Sook
MS
Park
Young In
YI
Jeong
Ha Sook
HS
Kim
Dong-Hyun
DH
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D034181:Epimedium / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
D005419:Flavonoids; D006027:Glycosides; C106053:epimedin B; C106054:epimedin C; C021304:hyperoside; D011794:Quercetin; C056599:icariin