title: [Fluid shear stress increases the Ca2+ concentration in bone-marrow derived osteoclast-like cells].
abstract: OBJECTIVE
  To study the change of Ca2+ density in cultured osteoclast-like cells in response to fluid shear stress.
  
  
  METHODS
  Laser scanning confocal microscope and fluorescent probe were used to detect the free Ca2+ in osteoclast-like cells before and after undergoing fluid shear stress. The images were analyzed and compared with image software.
  
  
  RESULTS
  At 37 degrees C the free Ca2+ in osteoclast-like cells could be labelled effectively with 10 micromol/L Fluo-3/AM. Compared with contol group, the average intensity of Ca2+ fluorescent signal in osteoclast-like cells undergoing fluid shear stress increased significantly.
  
  
  CONCLUSIONS
  The Cal2+ concentration in bone-marrow derived osteoclast-like cells is sensitive to fluid shear stress, which suggests osteoclast-like cells modulate their function in response to fluid shear stress through the change of free Ca2+ concentration.
authors: Dept. of Prosthodontics, Beijing Stomatologicul Hospital, Capital Medical University, Beijing 100050, China.
authors: Ming
authors: M
authors: Chen
authors: Xing
authors: X
authors: Liang
authors: Ying
authors: Y
authors: Wen
authors: Bao-jing
authors: BJ
authors: Bai
authors: Meng-lu
authors: ML
authors: Huang
authors: Wei-min
authors: WM
authors: Gao
mesh_terms: D000814:Aniline Compounds; D001853:Bone Marrow; D001854:Bone Marrow Cells; D002118:Calcium; D002478:Cells, Cultured; D010010:Osteoclasts; D013314:Stress, Mechanical; D014966:Xanthenes
chemical_list: D000814:Aniline Compounds; D014966:Xanthenes; C059715:Fluo-3; D002118:Calcium