Screening of antioxidant compounds during sprouting of Brassica oleracea L. var. costata DC.
The changes in antioxidant compounds of Brassica oleracea L. var. costata DC seeds were monitored during the first twelve days of seedling development. Sprouts were screened at time intervals of two days for phenolic compounds and organic acids. The identified phenolic compounds included esters of sinapic acid with glucose, gentiobiose and kaempferol, as well as sinapoylcholine. The organic acids were oxalic, aconitic, citric, pyruvic, malic, shikimic, and fumaric acids. During germination, a depletion of phenolic compounds was observed, although no qualitative changes were seen. Among individual compounds, kaempferol, choline and glucose esters of sinapic acid showed a marked decrease between days two and six, whereas the changes in gentiobiose esters of sinapic acid were smaller. The total organic acids content increased rapidly during the first four days, with less significant variations thereafter. Malic acid, the major organic acid found in sprouts, greatly contributed to this result though oxalic, pyruvic, and fumaric acids also increased in the same manner. In contrast, aconitic, citric and shikimic acids showed decreases between days two and twelve of germination.
Sousa
Carla
C
REQUIMTE/Serviço de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha 164, 4050-047 Porto, Portugal.
Lopes
Graciliana
G
Pereira
David M
DM
Taveira
Marcos
M
Valentão
Patrícia
P
Seabra
Rosa M
RM
Pereira
José A
JA
Baptista
Paula
P
Ferreres
Federico
F
Andrade
Paula B
PB
D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism; D001937:Brassica / Q000254:growth & development*; D002264:Carboxylic Acids / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D018547:Plant Stems / Q000254:growth & development; D012639:Seeds / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism
D000975:Antioxidants; D002264:Carboxylic Acids; D010636:Phenols