title: Gene profiles during root canal treatment in experimental rat periapical lesions.
abstract: The purpose of this study was to profile gene expression in periapical lesions during root canal treatment (RCT). Periapical lesions were induced experimentally by exposing the pulp in Sprague-Dawley rats. After 3 wk, the animals received root canal filling (RCF) and were sacrificed 1 or 4 wk later. From the periapical tissues, total RNA was extracted and processed for cDNA-microarray analysis. The lesions were histologically and radiographically confirmed to expand 4 wk after pulp exposure (inflammation phase) and to stabilize 4 wk after RCF (healing phase). In approximately 30,000 genes on the microarray, 203 genes were up-regulated to more than 5-fold (e.g., IL-1beta), and 864 genes were down-regulated to less than 20% of baseline level (e.g., caspase 8) in inflammation phase. Compared with inflammation phase, we found that 133 genes were up-regulated (e.g., IL-1alpha) and 50 genes were down-regulated (e.g., defensin alpha5) in healing phase. Corresponding to the gene expression profiles, accumulation of IL-1alpha and IL-1beta was observed in the periapical lesions by immunohistochemistry. These gene profiles might be useful in diagnosing the healing process of periapical lesions.
authors: Department of Pathophysiology-Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors: Zulema Rosalia Arias
authors: ZR
authors: Martinez
authors: Koji
authors: K
authors: Naruishi
authors: Keisuke
authors: K
authors: Yamashiro
authors: Fumio
authors: F
authors: Myokai
authors: Teruo
authors: T
authors: Yamada
authors: Kaori
authors: K
authors: Matsuura
authors: Naoko
authors: N
authors: Namba
authors: Hideo
authors: H
authors: Arai
authors: Junzo
authors: J
authors: Sasaki
authors: Yoshimitsu
authors: Y
authors: Abiko
authors: Shogo
authors: S
authors: Takashiba
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D015536:Down-Regulation; D020869:Gene Expression Profiling; D007124:Immunoenzyme Techniques; D007375:Interleukin-1 / Q000096:biosynthesis; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010485:Periapical Periodontitis / Q000235:genetics* / Q000628:therapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012390:Root Canal Therapy; D015854:Up-Regulation; D014945:Wound Healing / Q000235:genetics*
chemical_list: D007375:Interleukin-1