Peptide-based fluorescent sensors of protein kinase activity: design and applications.
Protein kinases control the flow of information through cell-signaling pathways. A detailed analysis of their behavior enhances our ability to understand normal cellular states and to devise therapeutic interventions for diseases. The design and application of "Environmentally-Sensitive", "Deep-Quench" and "Self-Reporting" sensor systems for studying protein kinase activity are described. These sensors allow real-time activity measurements in a continuous manner for a wide variety of kinases. As these sensors can be adapted from an in vitro screen to imaging kinase activity in living cells, they support both preliminary and later stages of drug discovery.
Sharma
Vyas
V
Department of Biochemistry, The Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Wang
Qunzhao
Q
Lawrence
David S
DS
D000818:Animals; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D008856:Microscopy, Fluorescence; D015336:Molecular Probe Techniques; D010455:Peptides / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*
D005456:Fluorescent Dyes; D010455:Peptides; D011494:Protein Kinases