RAPD analysis of DNA polymorphism in Turkish sheep breeds.
The genetic diversity and phylogenetic relationships of 108 individual sheep from three Turkish sheep breeds (Kivircik, Gökçeada, and Sakiz) were studied using RAPD analysis. Polymorphisms within and between populations were assayed using 15 random primers, and 82 loci were amplified ranging from 250 to 2,500 bp. The percentage of polymorphic loci was found to be 80.49, 78.05, and 73.17%, for Kivircik, Gökçeada, and Sakiz sheep breeds, respectively. Total genetic diversity was 0.2265, and the average coefficient of genetic differentiation was 0.1181. Genetically, the Gökçeada breed was more closely related to the Sakiz breed than to the Kivircik breed.
Elmaci
Cengiz
C
Animal Science Department, Faculty of Agriculture, Uludag University, 16059, Bursa, Turkey. elmaci@uludag.edu.tr
Oner
Yasemin
Y
Ozis
Seniz
S
Tuncel
Erdogan
E
D000818:Animals; D001483:Base Sequence; D001947:Breeding; D004247:DNA / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D014644:Genetic Variation; D005828:Genetics, Population; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012756:Sheep / Q000145:classification / Q000235:genetics*; D014421:Turkey
D017931:DNA Primers; D004247:DNA