Energy-dependent kinetic method: application to the multicompetitive fragmentation pathways of protonated peptides.
A variation of the kinetic method for the analysis of fragmentation patterns in mass spectra is proposed. The procedure presents three notable features: no evaluation of the effective temperature of the parent ion is required; the ratio of the activation energies for all competitive channels at play are provided; and the measurement is not biased by the mass discrimination of the instrument. The method is based on the analysis of mass spectra recorded as a function of both the excitation energy and the excitation time. Collision-activated dissociation of protonated Leu-enkephalin achieved in a quadrupolar ion trap and analyzed with this method is presented.
Compagnon
Isabelle
I
Université de Lyon, Université Lyon 1, CNRS, LASIM UMR 5579, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne cedex, France.
Dugourd
Philippe
P
D004745:Enkephalins / Q000032:analysis / Q000737:chemistry*; D007477:Ions; D007700:Kinetics; D013058:Mass Spectrometry; D018377:Neurotransmitter Agents / Q000032:analysis / Q000737:chemistry*; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry*; D011522:Protons; D013816:Thermodynamics
D004745:Enkephalins; D007477:Ions; D018377:Neurotransmitter Agents; D010446:Peptide Fragments; D011522:Protons