Determination of triethylenetetramine (TETA) and its metabolites in human plasma and urine by liquid chromatography-mass spectrometry (LC-MS).
A liquid chromatography-mass spectrometry (LC-MS) method has been developed to measure triethylenetetramine (TETA) and its metabolites in human samples. We identified two metabolites of TETA, N1-acetyltriethylenetetramine (MAT) and N1,N10-diacetyltriethylenetetramine (DAT), the latter being novel. We further developed this LC-MS method for the measurement of TETA and these metabolites in human plasma and urine in a single injection. Separation of analytes was achieved on a cyano column using 15% acetonitrile, 85% water (18 M Omega), and 0.1% heptafluorobutyric acid as the mobile phase. Simultaneous MS detection was performed at [M+H]+ values of 147, 189, 231 and 245, corresponding to TETA, MAT, DAT, and N1-acetylspermine as the internal standard, respectively. This method was successfully applied to measure TETA, MAT and DAT in plasma and urine of humans receiving oral drug treatment.
Lu
Jun
J
School of Biological Sciences, University of Auckland, Auckland, New Zealand.
Chan
Yi-Kai
YK
Poppitt
Sally D
SD
Cooper
Garth J S
GJ
D002138:Calibration; D002614:Chelating Agents / Q000032:analysis* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D012680:Sensitivity and Specificity; D014266:Trientine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000652:urine
D002614:Chelating Agents; C525989:N1,N10-diacetyltriethylenetetramine; C525988:N1-acetyltriethylenetetramine; D014266:Trientine