Histochemistry for placenta research: theory and application.
Histochemical techniques have contributed significantly to advances in placental biology and cell biology. In this mini-review, we describe recent advances in histochemical technologies and show how these technologies can profoundly improve our understanding of placenta morphological function related to health and disease. Fundamental theories and applications of five separate methods discussed here are 1) tissue-based polymerase chain reaction by laser microdissection, 2) a novel antigen retrieval method using citraconic anhydride plus heating, 3) immunohistochemical detection of Lewis-related antigen expression and galectin-1 binding in the human placenta, 4) confocal microscopy analysis of IgG transport in placental trophoblasts, and 5) high-resolution immunofluorescence and correlative microscopy using ultrathin cryosections in placental research. This review article is based on a presentation given in a workshop entitled Histochemistry: Theory and Application at the 12th International Federation of Placenta Associations Meeting held in Kobe, Japan, on September 9, 2006.
Takizawa
Toshihiro
T
Department of Molecular Anatomy, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan. t-takizawa@nms.ac.jp
Eguchi
Hidetaka
H
Namimatsu
Shigeki
S
Jeschke
Udo
U
Fuchs
Renate
R
Robinson
John M
JM
D005260:Female; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D010920:Placenta / Q000166:cytology* / Q000378:metabolism*; D011247:Pregnancy