The effect of prior exercise on ex vivo induction of heme oxygenase-1 in human lymphocytes.
It was postulated that prior demanding exercise would suppress the induction of the oxidant-responsive protein Heme Oxygenase-1 (HO-1) in mononuclear cells following subsequent ex vivo H(2)O(2) treatment. Eight male subjects completed two trials in a randomized order (one rest and one exercise) and ex vivo HO-1 protein induction was determined following H(2)O(2) treatment in lymphocytes and monocytes before and after each trial using a newly developed and reproducible assay. Lymphocytes obtained 2 h post-exercise showed a modest reduction in HO-1 protein expression in response to ex vivo treatment with H(2)O(2) (p<0.05). The plasma concentration of the HO-1 suppressor alpha1-antitrypsin increased immediately post-exercise (p<0.05) and it is tentatively suggested that this may explain the modest transient reduction in ex vivo HO-1 protein induction in lymphocytes in response to an independent oxidant challenge following a prior bout of demanding exercise.
Markovitch
Daniella
D
Sport & Exercise Science, School for Health, University of Bath, Bath, UK.
Tyrrell
Rex M
RM
Thompson
Dylan
D
D000328:Adult; D001671:Biochemistry / Q000379:methods; D002470:Cell Survival; D015444:Exercise; D015971:Gene Expression Regulation, Enzymologic; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000378:metabolism; D016877:Oxidants / Q000737:chemistry; D015203:Reproducibility of Results; D000515:alpha 1-Antitrypsin / Q000737:chemistry
D016877:Oxidants; D000515:alpha 1-Antitrypsin; D006861:Hydrogen Peroxide; D051547:Heme Oxygenase-1