title: Proteomic characterization of the site-dependent functional difference in the rat small intestine.
abstract: To investigate the site-dependent functional difference in the small intestine, proteomic analysis was carried out on the three distinct parts of the rat small intestine. Male Wistar rats (7 weeks old) were fed a semi-purified diet ad libitum for 1 week. Intestinal tissues from the proximal, middle and distal regions of the small intestine were subjected to two-dimensional polyacrylamide gel electrophoresis, and the abundance of each spot was determined fluorometrically. MALDI-TOF/MS and LC-MS/MS analysis of the tryptic peptides were performed to identify the proteins. Many of the 180 identified proteins showed a distinctive distribution pattern along the small intestine. Glutathione S-transferase, Catechol O-methyltransferase and Villin 2 decreased gradually from the jejunum to the ileum, in contrast, non-specific dipeptidase and Keratin 19 increased gradually toward the ileum. The voltage-dependent anion channel 2 was most abundant in the duodenum while the L- and I-Fatty acid binding protein (FABP) and Cellular retinol binding protein (CRBP-II) were in the jejunum, and the Bile acid binding protein (BABP) was only observed in the ileum. The findings of these and of another proteins identified in this study may contribute to further understanding of the small intestinal function, and to clinical applications of small intestinal diseases.
authors: Laboratory for Nutritional Biochemistry, School of Agriculture, Meiji University, 1-1-1 Higashi-mita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.
authors: Gotaro
authors: G
authors: Iiizumi
authors: Yuko
authors: Y
authors: Sadoya
authors: Sayaka
authors: S
authors: Hino
authors: Naoki
authors: N
authors: Shibuya
authors: Hiroaki
authors: H
authors: Kawabata
mesh_terms: D000818:Animals; D015536:Down-Regulation; D004386:Duodenum / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D007082:Ileum / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D007421:Intestine, Small / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D007583:Jejunum / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D008297:Male; D009928:Organ Specificity / Q000235:genetics; D020543:Proteome / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000737:chemistry* / Q000502:physiology*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation / Q000502:physiology
chemical_list: D020543:Proteome