Reduction in oxalate-induced renal tubular epithelial cell injury by an extract from Quercus salicina Blume/Quercus stenophylla Makino.
Urocalun, a herbal medicine prepared from an extract of Quercus salicina Blume/Quercus stenophylla Makino (QS extract), has been clinically used for the treatment of urolithiasis in Japan since 1969. In the present study, the effects of QS extract on oxalate-induced cell injury and NADPH-induced superoxide anion (O(2) (-)) production in the injured cells were investigated. Oxalate-induced cell injury was assessed by mitochondrial reduction of 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyltetrazolium bromide and leakage of lactate dehydrogenase into the extracellular fluid. When NRK-52E cells were injured by exposure to oxalate for 24 h, QS extract prevented the injury in a dose-dependent manner. In addition, QS extract suppressed the increase in NADPH-induced O(2) (-) production, or NADPH oxidase activity, in the homogenate of cells injured by oxalate exposure. These findings suggest that the reduction in oxalate-induced O(2) (-) production contributes to the cytoprotective effect of QS extract.
Moriyama
Manabu T
MT
Department of Urogenital Surgery, Kanazawa Medical University, Uchinada, Ishikawa, Japan. moriyama@kanazawa-med.ac.jp
Miyazawa
Katsuhito
K
Noda
Kumiko
K
Oka
Michiko
M
Tanaka
Mitsushi
M
Suzuki
Koji
K
D000818:Animals; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D007684:Kidney Tubules / Q000187:drug effects* / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010070:Oxalates / Q000009:adverse effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D029963:Quercus; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D052878:Urolithiasis / Q000188:drug therapy; D019459:Urothelium / Q000187:drug effects* / Q000378:metabolism
D010070:Oxalates; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D009249:NADP