Human neonatal dendritic cells are competent in MHC class I antigen processing and presentation.
Neonates are clearly more susceptible to severe disease following infection with a variety of pathogens than are adults. However, the causes for this are unclear and are often attributed to immunological immaturity. While several aspects of immunity differ between adults and neonates, the capacity of dendritic cells in neonates to process and present antigen to CD8+ T cells remains to be addressed. We used human CD8+ T cell clones to compare the ability of neonatal and adult monocyte-derived dendritic cells to present or process and present antigen using the MHC class I pathway. Specifically, we assessed the ability of dendritic cells to present antigenic peptide, present an HLA-E-restricted antigen, process and present an MHC class I-restricted antigen through the classical MHC class I pathway, and cross present cell-associated antigen via MHC class I. We found no defect in neonatal dendritic cells to perform any of these processing and presentation functions and conclude that the MHC class I antigen processing and presentation pathway is functional in neonatal dendritic cells and hence may not account for the diminished control of pathogens.
Gold
Marielle C
MC
Department of Pulmonary and Critical Care Medicine, Oregon Health & Science University, Portland, Oregon, USA. goldm@ohsu.edu
Robinson
Tammie L
TL
Cook
Matthew S
MS
Byrd
Laura K
LK
Ehlinger
Heather D
HD
Lewinsohn
David M
DM
Lewinsohn
Deborah A
DA
D017951:Antigen Presentation / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000821:virology; D005434:Flow Cytometry; D015789:HLA-A2 Antigen / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007371:Interferon-gamma / Q000276:immunology; D010750:Phosphoproteins / Q000276:immunology; D014616:Vaccinia virus / Q000254:growth & development / Q000276:immunology; D014763:Viral Matrix Proteins / Q000276:immunology
D015789:HLA-A2 Antigen; D015395:Histocompatibility Antigens Class I; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa; D007371:Interferon-gamma