title: Bone regeneration using a naturally grown HA/TCP carrier loaded with rh BMP-2 is independent of barrier-membrane effects.
abstract: The present study investigated whether bone regeneration and biomaterial replacement would be improved by loading of biogenous biphasic biomaterial scaffolds (HA/TCP ratio 30/70) with rhBMP-2, and whether the placement of three barrier membranes differing in structure and porosity (prototyped SLA Ti specimens, GORE RESOLUT Adapt specimens, and titanized TiMESH light specimens) would have a synergistic effect. A rabbit calvarial model was used for the implantation studies. Histological specimens were obtained after 12 weeks and evaluated quantitatively for differences between the various material combinations. Loading of the biomaterials with rhBMP-2 significantly enhanced the amount of regenerated bone and caused a pronounced biomaterial replacement. While BMP-induced bone had formed uniformly over the surgical defects, bone regeneration in the absence of BMP depends on bone promotion from the margins of the defects toward the center. No positive effect on bone regeneration was seen for any of the placed barrier membranes. While the present study had shown that rhBMP-2 loading significantly increases bone regeneration using the investigated biomaterial, barrier-membrane placement may be useful in predetermining the final shape of the regenerative site but provides no additional beneficial impact on the amount and quality of the bone regeneration induced by rhBMP-2.
authors: Hospital of Cranio-Maxillofacial and Oral Surgery, Medical University of Vienna, Vienna, Austria. christian.schopper@meduniwien.ac.at
authors: C
authors: C
authors: Schopper
authors: D
authors: D
authors: Moser
authors: E
authors: E
authors: Spassova
authors: W
authors: W
authors: Goriwoda
authors: G
authors: G
authors: Lagogiannis
authors: B
authors: B
authors: Hoering
authors: R
authors: R
authors: Ewers
authors: H
authors: H
authors: Redl
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D002130:Calcium Phosphates / Q000378:metabolism*; D017886:Durapatite / Q000378:metabolism*; D006801:Humans; D020007:Implants, Experimental; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology*; D012886:Skull / Q000166:cytology / Q000187:drug effects; D054457:Tissue Scaffolds; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
chemical_list: C525718:BMP2 protein, human; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D002130:Calcium Phosphates; D008567:Membranes, Artificial; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D017886:Durapatite; C018392:tricalcium phosphate