title: [Interest of the disk diffusion method for screening Clostridium difficile isolates with decreased susceptibility to antibiotics].
abstract: OBJECTIVE
  In vitro determination of Clostridium difficile susceptibility to antibiotics is not routinely performed. The aim of this study was to evaluate the performance of antibiotic susceptibility determination with the disk diffusion method for screening C. difficile isolates with decreased susceptibility to antibiotics.
  
  
  METHODS
  Thirty-six C. difficile isolates (toxigenic or not) isolated in 2005 and 2006 from three hospitals Assistance publique-Hôpitaux de Paris (Jean-Verdier, René-Muret, Beaujon) were studied by disk diffusion method with 14 antibiotics. Mueller-Hinton agar supplemented with sheep blood (Bio-Rad*) were swabbed with a C. difficile suspension at 1 McFarland. To check the results obtained with the disk diffusion method, Minimal Inhibitory Concentration (MIC) were performed respectively with E-test for glycopeptides and metronidazole and with the agar dilution reference method and E-test for new molecules with a potential activity against anaerobes: imipenem, ertapenem, linezolid and moxifloxacin.
  
  
  RESULTS
  The decreased susceptibility (resistant and intermediate) observed was 40% for amoxicillin-clavulanate, 60% for piperacillin-tazobactam, 100% for ceftriaxone, 81% for imipenem, 61% for ertapenem, 2% for chloramphenicol, 34% for erythromycin, 90% for lincomycin, 2% for linezolid, 98% for levofloxacin, 17% for moxifloxacin and 0% for vancomycin, teicoplanin and metronidazole. The results obtained with the disk diffusion method were compared to MICs obtained with E-test and reference method.
  
  
  CONCLUSIONS
  The disk diffusion method seems to be a good method to detect isolates suspected to have a decreased susceptibility and consequently to reduce MIC determinations.
authors: Laboratoire de microbiologie, hôpital Jean-Verdier, Assistance publique-Hôpitaux de Paris, avenue du 14-Juillet, 93140 Bondy, France. isabelle.poilane@jvr.aphp.fr
authors: I
authors: I
authors: Poilane
authors: F
authors: F
authors: Bert
authors: P
authors: P
authors: Cruaud
authors: M-H
authors: MH
authors: Nicolas-Chanoine
authors: A
authors: A
authors: Collignon
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016360:Clostridioides difficile / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D004058:Diffusion; D004305:Dose-Response Relationship, Drug; D005602:France; D006761:Hospitals; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000639:trends*
chemical_list: D000900:Anti-Bacterial Agents