title: Activity of immobilized trypsin in the layer structure of polyelectrolyte microcapsules (PEMC).
abstract: Polyelectrolyte microcapsules composed by using the LbL technique on stabilized RBC as templates were coated with up to ten layer pairs of trypsin/PSS or trypsin/alginate. The trypsin layer growth was confirmed by particle electrophoresis, confocal laser scanning microscopy, flow cytometry, and protein determination according to Lowry. In the coating series with trypsin/PSS, the amount of immobilized enzyme was larger than that with trypsin/alginate. The enzyme immobilization led to activity reduction of up to 90% compared to that of the same enzyme amount in the solution. No significant differences between the activities of trypsin immobilized in combination with PSS and with alginate were found.
authors: Institute of Transfusion Medicine, Charité-Universitätsmedizin Berlin, D-10098 Berlin, Germany.
authors: Eike
authors: E
authors: Garbers
authors: Rita
authors: R
authors: Mitlöhner
authors: Radostina
authors: R
authors: Georgieva
authors: Hans
authors: H
authors: Bäumler
mesh_terms: D000327:Adsorption; D000464:Alginates / Q000378:metabolism; D002214:Capsules / Q000737:chemistry* / Q000378:metabolism*; D004573:Electrolytes / Q000378:metabolism*; D004586:Electrophoresis; D004800:Enzymes, Immobilized / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D006801:Humans; D011137:Polystyrenes / Q000378:metabolism; D012996:Solutions; D014357:Trypsin / Q000378:metabolism*
chemical_list: D000464:Alginates; D002214:Capsules; D004573:Electrolytes; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D011137:Polystyrenes; D012996:Solutions; C003321:polystyrene sulfonic acid; D020723:Glucuronic Acid; D014357:Trypsin; D016650:Fluorescein-5-isothiocyanate