Wounding and pathogen infection induce a chloroplast-targeted lipoxygenase in the common bean (Phaseolus vulgaris L.).
Chloroplastic LOXs are implicated in the biosynthesis of oxylipins like jasmonic acid and C6 volatiles among others. In this study, we isolated the cDNA of a novel chloroplast-targeted Phaseolus vulgaris LOX, (PvLOX6). This gene is highly induced after wounding, non-host pathogen infection, and by signaling molecules as H2O2, SA, ethylene and MeJA. The phylogenetic analysis of PvLOX6 showed that it is closely related to chloroplast-targeted LOX from potato (H1) and tomato (TomLOXC); both of them are implicated in the biosynthesis of C6 volatiles. Induction of PvLOX6 mRNA by wounding ethylene and jasmonic acid on the one side, and non-host pathogen, salicylic acid on the other indicates that common bean uses the same LOX to synthesize oxylipins in response to different stresses.
Porta
Helena
H
Departmento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Cuernavaca, Morelos, C. P. 6100, Mexico. helena@ibt.unam.mx
Figueroa-Balderas
Rosa Elia
RE
Rocha-Sosa
Mario
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002736:Chloroplasts / Q000378:metabolism*; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology; D004790:Enzyme Induction; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D008084:Lipoxygenase / Q000378:metabolism*; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000494:pharmacology; D027805:Phaseolus / Q000187:drug effects / Q000378:metabolism*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*
D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid; C036216:ethylene; D008084:Lipoxygenase