title: Electrophoretic method for assessment of substrate promiscuity of a heterogeneous biocatalyst using an area imaging ultraviolet detector.
abstract: We report a new electrophoretic set-up and method for rapid specificity screening of an immobilised enzyme against a range of substrates present in a mixture. The penicillinase-catalysed reaction is carried out on-the-fly, following separation of putative substrates and preceding separation of the reaction products. The new active pixel sensor detector gives an option of using multiple detection windows on a single flow line and enables efficient on-line monitoring of this heterogeneous biocatalytic process with multiple putative substrates injected simultaneously.
authors: Department of Chemistry, University of York, Heslington, York, UKYO10 5DD.
authors: Pawel L
authors: PL
authors: Urban
authors: Edmund T
authors: ET
authors: Bergström
authors: David M
authors: DM
authors: Goodall
authors: Sreedevi
authors: S
authors: Narayanaswamy
authors: Neil C
authors: NC
authors: Bruce
mesh_terms: D019149:Bioreactors; D002384:Catalysis; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D004798:Enzymes / Q000032:analysis*; D010405:Penicillinase / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D004798:Enzymes; D010405:Penicillinase