title: Malassezia otitis externa in the dog: the effect of heat-fixing otic exudate for cytological analysis.
abstract: This study was conducted on 32 dogs with Malassezia otitis externa to determine the effect of heat-fixing otic exudate on cytological analysis. Malassezia infection was confirmed by cytological examination of otic exudate. Otic discharge collected with cotton swabs was then rolled onto glass slides. One slide per dog was heat-fixed prior to staining; the other slide was not heat-fixed. The number of yeast in 10 oil-immersion fields (1000 x magnification) was counted for both slides from each dog. Heat-fixing did not systematically cause either increased or decreased numbers of Malassezia on cytology of otic exudate.
authors: Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
authors: J S
authors: JS
authors: Griffin
authors: D W
authors: DW
authors: Scott
authors: H N
authors: HN
authors: Erb
mesh_terms: D000818:Animals; D002571:Cerumen / Q000166:cytology* / Q000382:microbiology; D003881:Dermatomycoses / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis / Q000473:pathology*; D004285:Dogs; D004424:Ear Canal / Q000166:cytology / Q000382:microbiology; D005260:Female; D008290:Malassezia / Q000302:isolation & purification*; D008297:Male; D010032:Otitis Externa / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary; D013194:Staining and Labeling / Q000662:veterinary