IL-8 and pH values in exhaled condensate after antibiotics in cystic fibrosis children.
Interleukin (IL)-8 is a major factor in inflammatory response and the IL-8 levels in exhaled breath condensate (EBC) may be used as a marker of airway inflammation. Airway acidification is implicated in the pathophysiology of obstructive airway diseases and pH EBC values have been used as a marker of airway acidification. The aim of our study is to investigate whether IL-8 and pH levels in EBC of cystic fibrosis (CF) children with respiratory exacerbations change after antibiotic treatment. Lung function, IL-8 and pH EBC values were measured in fifteen CF children (mean age 11 years) with acute exacerbation before (T0) and after two weeks (T1) of antibiotic treatment. IL-8 and pH values were compared by paired t-test. A p less than 0.05 was considered significant. IL-8 EBC levels decreased after antibiotic treatment (T0 0.36+/-0.03pg/ml vs T1 0.28+/-0.03pg/ml; p=0.03) and pH values increased (T0 7.36+/-0.09 vs T1 7.61+/-0.08; p=0.04). Results suggest possible application of EBC as a non-invasive tool to monitor efficacy of antibiotic treatment in CF patients.
Bodini
A
A
Dipartimento di Pediatria, Università di Verona, Verona, Italy. alessandro.bodini@univr.it
D'Orazio
C
C
Peroni
D G
DG
Corradi
M
M
Zerman
L
L
Folesani
G
G
Assael
B M
BM
Boner
A L
AL
Piacentini
G L
GL
D000293:Adolescent; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D001424:Bacterial Infections / Q000150:complications / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology; D015415:Biomarkers / Q000032:analysis; D001944:Breath Tests; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000150:complications / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016209:Interleukin-8 / Q000032:analysis* / Q000276:immunology; D012129:Respiratory Function Tests; D012141:Respiratory Tract Infections / Q000150:complications / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology
D000900:Anti-Bacterial Agents; D015415:Biomarkers; D016209:Interleukin-8