Biochemical Characterization of SFC-1, a class A carbapenem-hydrolyzing beta-lactamase.
The carbapenem-hydrolyzing beta-lactamase SFC-1 from Serratia fonticola UTAD54 was overexpressed in Escherichia coli, purified, and characterized. The enzyme exhibited an apparent molecular mass of 30.5 kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SFC-1 hydrolyzes penicillins, cephalosporins, aztreonam, and carbapenems and is inhibited by clavulanic acid, sulbactam, and tazobactam.
Fonseca
Fátima
F
Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.
Sarmento
Ana Cristina
AC
Henriques
Isabel
I
Samyn
Bart
B
van Beeumen
Jozef
J
Domingues
Pedro
P
Domingues
Maria Rosário
MR
Saavedra
Maria José
MJ
Correia
António
A
D001398:Aztreonam / Q000378:metabolism; D015780:Carbapenems / Q000378:metabolism*; D002384:Catalysis / Q000187:drug effects; D002511:Cephalosporins / Q000378:metabolism; D019818:Clavulanic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D008970:Molecular Weight; D010397:Penicillanic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D010406:Penicillins / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012705:Serratia / Q000201:enzymology* / Q000235:genetics; D013407:Sulbactam / Q000494:pharmacology; D000078142:Tazobactam; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D015780:Carbapenems; D002511:Cephalosporins; D010406:Penicillins; D011994:Recombinant Proteins; D019818:Clavulanic Acid; D010397:Penicillanic Acid; D001618:beta-Lactamases; D001398:Aztreonam; D013407:Sulbactam; D000078142:Tazobactam