Multichannel SPR biosensor for detection of endocrine-disrupting compounds.
A surface plasmon resonance (SPR) biosensor for simultaneous detection of multiple organic pollutants exhibiting endocrine-disrupting activity, namely atrazine, benzo[a]pyrene, 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-nonylphenol, is reported. The biosensor utilizes a multichannel SPR sensor based on wavelength modulation of SPR and wavelength division multiplexing (WDM) of sensing channels, antibodies as biorecognition element and a competitive immunoassay detection format. An analysis time of 45 min (including 30-min incubation of the sample with antibodies) and limits of detection as low as 0.05, 0.07, 0.16 and 0.26 ng mL(-1) are demonstrated for benzo[a]pyrene, atrazine, 2,4-D and 4-nonylphenol, respectively. The biosensor is also shown to be regenerable and suitable for repeated use.
Dostálek
J
J
Institute of Photonics and Electronics, Czech Academy of Sciences, Chaberská 57, 18251 Prague, Czech Republic.
Pribyl
J
J
Homola
J
J
Skládal
P
P
D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis; D001280:Atrazine / Q000032:analysis; D001564:Benzo(a)pyrene / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D052244:Endocrine Disruptors / Q000032:analysis*; D007118:Immunoassay / Q000379:methods; D010636:Phenols / Q000032:analysis; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
D052244:Endocrine Disruptors; D010636:Phenols; D015084:2,4-Dichlorophenoxyacetic Acid; D001564:Benzo(a)pyrene; C041594:4-nonylphenol; D001280:Atrazine