[Effects of transforming growth factor-beta1 gene therapy on bone rarefaction around endosseous implant].
OBJECTIVE
To investigate the effects of transforming growth factor-beta1 (TGF-beta1) gene therapy on bone defect and bone rarefaction around endosseous implant.


METHODS
The primary cultured bone marrow derived stroma cells (BMSCs) was transfected by plasmid pCDNA3.1(+) -TGF-beta1, and was adhered with polylactic-co-glycolic acid (PLGA) for constructing TGF-beta1 gene-modified artificial bone. The model of rats with placed titanium implants in the proximal metaphyses of the tibiae after ovariectomy was made. The TGF-beta1 gene-modified artificial bone (experimental group), BMSCs-PLGA compound artificial bone (control group) and nothing (blank control group) were placed in the bone defect around implant. The tibiae were examined by decalcified sections with immunohistochemical method and histological analysis methods at intervals of 4 and 8 weeks after implant surgery in order to detect the expression of TGF-beta1 in new bone adjacent to the implant and the healing of the bone defect around the implant.


RESULTS
The expression level of TGF-beta1 of experimental group was higher than that of control group and blank control group at the 4th week. The histological analysis indicated that the gene-modified artificial bone had stronger osetogenic potential than others.


CONCLUSIONS
TGF-beta1 gene-modified artificial bone promotes the repair of the bone defect around titanium implants in osteoporotic rats.
Cao
Ying-guang
YG
Dept. of Stomatology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China.
Wang
Rong
R
Song
Ke
K
Xiong
Zong-qiang
ZQ
Du
Jian-ming
JM
Wang
Hua-jun
HJ
D000818:Animals; D001842:Bone and Bones; D002478:Cells, Cultured; D003758:Dental Implantation, Endosseous; D015921:Dental Implants; D005260:Female; D015316:Genetic Therapy; D019736:Prostheses and Implants; D051381:Rats; D017154:Stromal Cells; D014025:Titanium; D014162:Transfection; D053773:Transforming Growth Factor beta1
D015921:Dental Implants; D053773:Transforming Growth Factor beta1; D014025:Titanium