In vitro susceptibilities of Candida spp. to Panomycocin, a novel exo-beta-1,3-glucanase isolated from Pichia anomala NCYC 434.
Panomycocin, the killer toxin of Pichia anomala NCYC 434 (K5), is a 49 kDa monomeric glycoprotein with exo-beta-1,3-glucanase activity (patent pending). In this study we evaluated the in vitro activity of panomycocin against a panel of 109 human isolates of seven different pathogenic Candida spp. using microdilution and time-kill methods. Panomycocin was most active against C. tropicalis, C. pseudotropicalis and C. glabrata with MIC(90) values of 1 microg/ml. It displayed significant activity against C. albicans and C. parapsilosis with MIC(90) values of 4 and 2 microg/ml, respectively. For C. krusei, the MIC(90) value was 8 microg/ml. Panomycocin was fungicidal against all the tested Candida spp. The MFC values were only one or 2 dilutions higher than the MICs with the exception of C. krusei isolates with MFCs greater than or equal to 4xMIC. Results of this study indicated that panomycocin could be considered as a natural antifungal agent against Candida infections and has significant potential for further investigation.
Izgü
Fatih
F
Department of Biological Sciences, Middle East Technical University, Ankara, Turkey. izgu@metu.edu.tr
Altinbay
Demet
D
Türeli
Akif Emre
AE
D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D025141:Drug Resistance, Fungal; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009183:Mycotoxins / Q000302:isolation & purification / Q000494:pharmacology*; D010843:Pichia / Q000737:chemistry*
D000935:Antifungal Agents; D009183:Mycotoxins; D006026:Glycoside Hydrolases; C526433:panomycocin protein, Pichia anomala