The presence and distribution of gonadotropin-releasing hormone-liked factor in the central nervous system of the black tiger shrimp, Penaeus monodon.
The distribution and presence of gonadotropin-releasing hormone (GnRH) in the central nervous system (CNS) of Penaeus monodon were examined by immunocytochemistry, high performance liquid chromatography (HPLC), and radioimmunoassay (RIA). We demonstrated the existence of octopus (oct)GnRH-liked immunoreactivity (ir-octGnRH) and lamprey (l)GnRH-III-liked immunoreactivity (ir-lGnRH-III) in cell bodies of medium-sized neurons of the anterior part (protocerebrum) of the supraesophageal ganglion (brain). In addition, only the ir-octGnRH was detected in the nerve fibers located in the brain and segmental ganglia (subesophageal, thoracic, and abdominal ganglia). Moreover, some branches of these fibers also innervated the neurons in the middle (deutrocerebrum), posterior (tritocerebrum) brain and segmental ganglia. There was no ir-lGnRH-I and ir-salmon (s)GnRH detected in the shrimp CNS. The results from HPLC and RIA showed ir-GnRH in the CNS using anti-lGnRH-III, but not with anti-mammalian (m)GnRH. The data from immunocytochemistry, HPLC and RIA suggest that ir-GnRH in shrimp may be more similar to octGnRH and lGnRH-III than the other forms. These findings support the hypothesis that GnRH-liked factor(s) may be an ancient peptide that also exists in this decapod crustacean.
Ngernsoungnern
Piyada
P
Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Ngernsoungnern
Apichart
A
Kavanaugh
Scott
S
Sobhon
Prasert
P
Sower
Stacia A
SA
Sretarugsa
Prapee
P
D000818:Animals; D002490:Central Nervous System / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000302:isolation & purification / Q000378:metabolism*; D007150:Immunohistochemistry; D008954:Models, Biological; D033561:Penaeidae / Q000378:metabolism*; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism*; D011863:Radioimmunoassay; D014018:Tissue Distribution
C045152:Gonadorelin-like peptides; D010455:Peptides; D007987:Gonadotropin-Releasing Hormone