title: Effect of antimicrobial compounds on Balamuthia mandrillaris encystment and human brain microvascular endothelial cell cytopathogenicity.
abstract: Cycloheximide, ketoconazole, or preexposure of organisms to cytochalasin D prevented Balamuthia mandrillaris-associated cytopathogenicity in human brain microvascular endothelial cells, which constitute the blood-brain barrier. In an assay for inhibition of cyst production, these three agents prevented the production of cysts, suggesting that the biosynthesis of proteins and ergosterol and the polymerization of actin are important in cytopathogenicity and encystment.
authors: School of Biological and Chemical Sciences, Birkbeck College, University of London, London WC1E 7HX, England, United Kingdom.
authors: Ruqaiyyah
authors: R
authors: Siddiqui
authors: Abdul
authors: A
authors: Matin
authors: David
authors: D
authors: Warhurst
authors: Monique
authors: M
authors: Stins
authors: Naveed Ahmed
authors: NA
authors: Khan
mesh_terms: D000199:Actins / Q000378:metabolism; D000563:Amebicides / Q000494:pharmacology*; D000656:Amoeba / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000469:parasitology; D004875:Ergosterol / Q000096:biosynthesis; D006801:Humans; D007654:Ketoconazole / Q000494:pharmacology
chemical_list: D000199:Actins; D000563:Amebicides; D015638:Cytochalasin D; D003513:Cycloheximide; D007654:Ketoconazole; D004875:Ergosterol