title: The effect of conjugation to gold nanoparticles on the ability of low molecular weight chitosan to transfer DNA vaccine.
abstract: Nonviral gene delivery systems based on conventional high molecular weight chitosans are efficient as DNA vaccine delivery system, but have poor physical properties such as aggregated shapes, low solubility at neutral pH, high viscosity at concentrations used for in vivo delivery and a slow onset of action. Furthermore, Chitosan oligomers shorter than 14 monomers units were recently found to form only weak complexes with DNA, resulting in physically unstable polyplexes in vitro and in vivo. Here, low molecular weight chitosans with an average molecular mass of 6kDa (Chito6) have been covalently attached to gold nanoparticles (GNPs), and the potency of the resulting Chito6-GNPs conjugates as vectors for the delivery of plasmid DNA has been investigated in vitro and in vivo. After delivery by intramuscular immunization in BALB/c mice, the Chito6-GNPs conjugates induced an enhanced serum antibody response 10 times more potent than naked DNA vaccine. Additionally, in contrast to naked DNA, the Chito6-GNPs conjugates induced potent cytotoxic T lymphocyte responses at a low dose.
authors: College of Life Science, Jilin University, Changchun 130012, PR China.
authors: Xianfeng
authors: X
authors: Zhou
authors: Xizhen
authors: X
authors: Zhang
authors: Xianghui
authors: X
authors: Yu
authors: Xiao
authors: X
authors: Zha
authors: Qiuan
authors: Q
authors: Fu
authors: Bin
authors: B
authors: Liu
authors: Xueyun
authors: X
authors: Wang
authors: Yan
authors: Y
authors: Chen
authors: Yue
authors: Y
authors: Chen
authors: Yaming
authors: Y
authors: Shan
authors: Yinghua
authors: Y
authors: Jin
authors: Yongge
authors: Y
authors: Wu
authors: Junqiu
authors: J
authors: Liu
authors: Wei
authors: W
authors: Kong
authors: Jiacong
authors: J
authors: Shen
mesh_terms: D000818:Animals; D002460:Cell Line; D048271:Chitosan / Q000097:blood / Q000737:chemistry* / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation; D006046:Gold / Q000737:chemistry*; D007273:Injections, Intramuscular; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D018625:Microscopy, Atomic Force; D008970:Molecular Weight; D014162:Transfection; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry* / Q000276:immunology*
chemical_list: D019444:Vaccines, DNA; D006046:Gold; D048271:Chitosan