[Evolution of antibiotic resistance of Streptococcus pneumoniae: results of Alsace observatory in 2005].
OBJECTIVE
Between 1st January 2005 and 31st December 2005, 232 strains of Streptococcus pneumoniae were collected in the Alsace county from participating laboratories (one from university hospital, 7 from general hospitals and 12 private laboratories) to assess their susceptibility to penicillin and evaluated serogroups of strains.


METHODS
The coordinating centre performed MICs by the reference agar dilution test, interpreted according to CA-SFM breakpoints. Others antibiotics (erythromycin, cotrimoxazole, tetracycline...) were tested by agar diffusion, ATB-PNEUMO gallery or VITEK gallery (BioMérieux, France) by each participating laboratory. Data were processed, using 4th dimension software.


RESULTS
Strains were collected from 151 blood samples, 38 ear pus, 11 cerebrospinal fluids, 8 pleural liquids and 24 representative pulmonary samples. The prevalence of pneumococci with decreased susceptibility to penicillin G (PDSP) is 35.1% (pulmonary samples excluded). The rate of PNSP decreases for all types of samples compared with other years of surveillance 2003 (44.0%). The rate of blood samples decreases for first time between the creation of Pneumococcal Observatory. The high-level resistance tend to decrease and began low. The PDSP are rather resistant to erythromycin, cotrimoxazole and fosfomycin. Among the PDSP, the most prevalent serotypes were 14, 19, 6 and 9.


CONCLUSIONS
Among pneumococcal strains, the rate of PDSP tend however to decrease in 2005 compared with 2003. The rate stays inferior to the observed rates in other French counties where the same decreasing is described.
Gravet
A
A
Laboratoire de microbiologie, hôpital Emile-Muller, 20, avenue du Docteur-Laennec, 68070 Mulhouse cedex, France. graveta@ch-mulhouse.fr
Camdessoucens
G
G
Murbach
V
V
Barrand
P
P
Boucher
A
A
Boulenc
A
A
De Briel
D
D
Delarbre
J-M
JM
Drzewinski
J-C
JC
Flipo
J-L
JL
Gherardi
C
C
Grawey
I
I
Gueudet
T
T
Heidt
A
A
Herzig
V
V
Izraelewicz
D
D
Jehl
F
F
Kientz
P
P
Lantz
V
V
Lemble
C
C
Pierrot
P
P
Rieder
C
C
Riehm
D
D
Tytgat
F
F
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001769:Blood / Q000382:microbiology; D001826:Body Fluids / Q000382:microbiology; D024881:Drug Resistance, Bacterial / Q000502:physiology*; D005602:France; D006801:Humans; D007753:Laboratories; D008826:Microbial Sensitivity Tests; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D013492:Suppuration / Q000382:microbiology; D013997:Time Factors
D000900:Anti-Bacterial Agents