title: Genotyping of Paracoccidioides brasiliensis directly from paraffin embedded tissue.
abstract: Based on polymorphisms of the gp43 precursor gene, genotyping of Paracoccidioides brasiliensis was achieved in 6 out of 10 paraffin-embedded tissue samples by modifying a nested PCR procedure used in the diagnosis of the fungal infection. Nine of the samples originated in Brazil. Three sequences were identical to a previously published consensus sequence identifying the P. brasiliensis isolates as members of the formerly named species 1. In contrast, two sequences revealed substitutions identical to isolates of the phylogenetic species 2. Applying the method to a lung biopsy from a proven case of paracoccidioidomycosis diagnosed in Austria, the gp43 sequence revealed substitutions so far only described in five strains originating from Venezuela. Combining travel history and result of this new method, the most probable country of origin of the infections could be identified. Since endemic mycosis are often diagnosed by histopathology only, our method could help to extend epidemiological studies of paracoccidioidomycosis.
authors: Departamento Patologia, Escola Paulista de Medicina, Universidade Federal de São Paulo (EPM-UNIFESP), St Paulo, SP, Brazil.
authors: Giannina
authors: G
authors: Ricci
authors: Ulrike
authors: U
authors: Zelck
authors: Fernando
authors: F
authors: Mota
authors: Cornelia
authors: C
authors: Lass-Flörl
authors: Marcello F
authors: MF
authors: Franco
authors: Ralf
authors: R
authors: Bialek
mesh_terms: D001706:Biopsy; D001938:Brazil; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D010228:Paracoccidioides / Q000235:genetics* / Q000302:isolation & purification; D010229:Paracoccidioidomycosis / Q000382:microbiology* / Q000473:pathology; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D006023:Glycoproteins