SV40 multiple tissue infection and asbestos exposure in a hyperendemic area for malignant mesothelioma.
To assess the presence of SV40 in malignant mesothelioma tissue, 19 formalin-fixed paraffin-embedded pleural cancer samples of patients from a hyperendemic area of northeastern Italy were analyzed retrospectively. A total of 48 other tissues from the malignant mesothelioma subjects were investigated. The SV40 load was determined by real-time quantitative PCR. Exposure to asbestos was evaluated through a careful review of the occupational history of patients, supplemented by histology and isolation of asbestos bodies. Three of 19 (15.8%) malignant mesothelioma tissues harbored SV40 genomic signals. Two patients with SV40-positive malignant mesothelioma had viral sequences in another tissue. Overall, 3 of 18 (16.7%) normal liver tissues tested positive for SV40, as did 1 of 8 (12.5%) kidney tissues. SV40 viral loads were higher in malignant mesothelioma than in normal cells (P = 0.045). This survey shows that SV40 sustains infections in multiple tissues in malignant mesothelioma patients from a geographic area affected with asbestos-related mesothelioma.
Comar
Manola
M
Department of Public Medicine Sciences, University of Trieste, Trieste, Italy.
Rizzardi
Clara
C
de Zotti
Renata
R
Melato
Mauro
M
Bovenzi
Massimo
M
Butel
Janet S
JS
Campello
Cesare
C
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001194:Asbestos / Q000009:adverse effects*; D003043:Cocarcinogenesis; D004279:DNA, Viral / Q000235:genetics; D019353:Endemic Diseases; D005260:Female; D006801:Humans; D008297:Male; D008654:Mesothelioma / Q000453:epidemiology / Q000209:etiology* / Q000821:virology; D008875:Middle Aged; D010997:Pleural Neoplasms / Q000453:epidemiology / Q000209:etiology* / Q000821:virology; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000150:complications* / Q000821:virology; D012189:Retrospective Studies; D013539:Simian virus 40 / Q000235:genetics*; D014412:Tumor Virus Infections / Q000150:complications* / Q000821:virology
D004279:DNA, Viral; D001194:Asbestos