Thy-1 is expressed in hepatic myofibroblasts and not oval cells in stem cell-mediated liver regeneration.
Thy-1, a marker of hematopoietic stem cells, has been reported to be expressed by oval cells proliferating during stem cell-mediated regeneration in rat liver, suggesting a relationship between the two cell populations. Consequently, Thy-1 has become an accepted cell surface marker to sort hepatic oval cells. In the present study we used the well-characterized 2-acetylaminfluorene/partial hepatectomy model to induce transit-amplification of hepatic oval cells in the regenerating liver and characterized Thy-1 expression using Northern hybridization, quantitative reverse transcriptase-polymerase chain reaction analysis, immunofluorescence confocal microscopy, and immunoelectronmicroscopy. We found that Thy-1 expression was induced during transit-amplification of the oval cell population, but Thy-1 mRNA was not present in the alpha-fetoprotein-expressing oval cells. Thy-1 protein was consistently present outside the basement membrane surrounding the oval cells. It overlapped frequently with smooth muscle actin staining. A similar cellular localization of the Thy-1 protein was found on human liver specimens with ductular reactions obtained from patients with fulminant liver failure. Furthermore, Thy-1 was expressed by myofibroblasts in experimental liver fibrosis models without oval cell proliferation. We conclude that Thy-1 is not a marker of oval cells but is present on a subpopulation of myofibroblasts/stellate cells.
Dezso
Katalin
K
First Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary.
Jelnes
Peter
P
László
Viktória
V
Baghy
Kornélia
K
Bödör
Csaba
C
Paku
Sándor
S
Tygstrup
Niels
N
Bisgaard
Hanne Cathrine
HC
Nagy
Peter
P
D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D008106:Liver Cirrhosis, Experimental / Q000378:metabolism / Q000473:pathology; D017114:Liver Failure, Acute / Q000378:metabolism / Q000473:pathology; D008115:Liver Regeneration; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D013234:Stem Cells / Q000502:physiology*; D018800:Thy-1 Antigens / Q000096:biosynthesis*
D018800:Thy-1 Antigens