Biological activities of extracts from endophytic fungi isolated from Garcinia plants.
Sixty-five crude extracts from 51 selected endophytic fungi isolated from Garcinia species were tested for various bioactivities. Eighty per cent of the fungal extracts from fermentation broths and mycelia displayed bioactivities: antimycobacterial (76.9%), antimalarial (14.1%), antiviral (16.7%), antioxidant (22.2%), antiproliferation (11.1% against NCI-H187 and 12.7% against KB cells), and cytotoxicity to Vero cells (40.0%). Based on internal transcribed spacer rRNA sequence analysis, 15 bioactive isolates were identified as Aspergillus, Botryosphaeria, Curvularia, Fusicoccum, Guignardia, Muscodor, Penicillium, Pestalotiopsis, and Phomopsis spp. One isolate (N24) was matched with an unidentified fungal endophyte. These results indicate that endophytic fungi isolated from Garcinia plants in Thailand are potential sources of various bioactive natural products.
Phongpaichit
Souwalak
S
Natural Products Research Unit, Department of Microbiology, Faculty of Science, Prince of Songkla University, Songkhla, Thailand. souwalak.p@psu.ac.th
Nikom
Jaru
J
Rungjindamai
Nattawut
N
Sakayaroj
Jariya
J
Hutadilok-Towatana
Nongporn
N
Rukachaisirikul
Vatcharin
V
Kirtikara
Kanyawim
K
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D000995:Antitubercular Agents / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D002460:Cell Line / Q000187:drug effects; D002522:Chlorocebus aethiops; D045424:Complex Mixtures / Q000494:pharmacology / Q000633:toxicity; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D005658:Fungi / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D029760:Garcinia / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D000962:Antimalarials; D000970:Antineoplastic Agents; D000975:Antioxidants; D000995:Antitubercular Agents; D000998:Antiviral Agents; D045424:Complex Mixtures; D004271:DNA, Fungal; D021901:DNA, Intergenic