[Study on molecular typing by pulsed-field gel electrophoresis of food Listeria monocytogenes isolates from eight province (municipalities) of China].
OBJECTIVE
To analyse the molecular types of Listeria (L.) monocytogenes, and to construct the standard China L. monocytogenes pulsed-field gel electrophoresis (PFGE) subtyping database, using the international standardized L. monocytogenes-PFGE protocol.


METHODS
118 L. monocytogenes strains isolated from 8 provinces or municipalities of China were subtyped according to L. monocytogenes-PFGE protocol.


RESULTS
118 strains of L. monocytogenes were divided into 39 subtypes. In the 39 subtypes, 37 strains (31.36%) were GX6A160004 pattern, showing it was the predominant Lm subtype in China.


CONCLUSIONS
Data from molecular typing suggested that the predominant Lm strains were distributed in different regions of China. PulseNet China-Lm database was constructed which was valuable for the molecular subtyping-based surveillance of L. monocytogenes.
Zhu
Ren-fa
RF
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Gong
Zhao-long
ZL
Ye
Chang-yun
CY
Cui
Zhi-gang
ZG
Jin
Dong
D
Ran
Lu
L
Xu
Ke
K
Wang
Lian-xiu
LX
Chen
Wei-wei
WW
D002681:China; D016521:Electrophoresis, Gel, Pulsed-Field; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny