Frequently made mistakes in electrophoresis.
In spite of text books, instrument manuals, product instructions, and web tutorials there are a number of erroneous protocols around, which lead repeatedly to issues during electrophoresis runs and to inadequate results. The relatively low resolution and short running time of miniformat systems often conceals these issues. However, in high-resolution 2-D electrophoresis in large format gels, one of the most important separation methods in Proteomics, the consequences of these mistakes become more obvious.
Westermeier
Reiner
R
GE Healthcare Life Sciences, Oskar-Schlemmer-Strasse 11, D-80807 Munich, Germany. reiner.westermeier@ge.com
D000180:Acrylic Resins / Q000138:chemical synthesis / Q000737:chemistry; D016477:Artifacts; D002021:Buffers; D004586:Electrophoresis / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005998:Glycine / Q000737:chemistry; D020836:Protein Structure, Quaternary; D013696:Temperature; D013997:Time Factors; D014325:Tromethamine / Q000737:chemistry
D000180:Acrylic Resins; D002021:Buffers; C016680:polyacrylamide gels; D014325:Tromethamine; D005998:Glycine