[Morphometry of osteoclasts in experimental fracture healing of rabbits].
This study was designed to investigate the effects of some Traditional Chinese Medicine (TCM) agents on bone resorption and morphometric features of osteoclasts as well as their relationships. TCM ShengGuZaiZaoSan and XianLingGuBao, were used to treat the experimental fracture. Thirty 6-month-old Chinchilla rabbits were used for the establishment of animal models each with a 3 mm bone defect in the middle of left radius as well as of right radius. These models were divided randomly into 3 groups : ShengGuZaiZaoSan Group (Group A), XianLingGuBao groups (Group B) and control-group (Group C). Every group was further divided into 2 subgroups: a former sacrificed group (14 days after operation) and a latter sacrificed group (31 days after operation). After the rabbits being killed, the samples of their undecalcified calli were subjected to the morphometry study of bone resorption and osteoclasts. Group A had more bone resorption, compared with Group B and C. Both Groups A and B exhibited some changed morphometric features of osteoclasts as compared with Group C (P < 0.05). Simple correlation analysis indicated that bone resorption is mainly correlated with osteoclast numbers, and that in individual group, bone resorption is correlated with osteoclast form factor, area and mean photodensity (P < 0.05). These allow us to conclude that ShengGuZaiZaoSan can increase bone resorption and accelerate bone remodeling by increasing osteoclast numbers at the former stage and can enhance osteoclast function at the latter stage. These changes are beneficial to fracture healing.
Wu
Jiaqi
J
Department of Orthopaedics, West China Hospital of Sichuan University, Chengdu 610041, China. wujiaqi15@163.com
Wu
Yuanying
Y
Jiang
Yiwei
Y
Li
Hongzhuan
H
Zhang
Xiaogang
X
Yang
Tianfu
T
D000818:Animals; D016723:Bone Remodeling / Q000187:drug effects; D001862:Bone Resorption / Q000503:physiopathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D017102:Fracture Healing / Q000187:drug effects* / Q000502:physiology; D008297:Male; D010010:Osteoclasts / Q000187:drug effects / Q000473:pathology*; D008517:Phytotherapy; D011817:Rabbits; D011885:Radius Fractures / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology*; D011897:Random Allocation
D004365:Drugs, Chinese Herbal