ROS production by adrenodoxin does not cause apoptosis in fission yeast.
We previously showed that production of reactive oxygen species (ROS) caused by overexpression of the mitochondrial electron transfer protein adrenodoxin (Adx) induces apoptosis in mammalian cells. In the fission yeast Schizosaccharomyces pombe, ROS are also produced in cells that undergo an apoptotic-like cell death, but it is not yet clear whether they are actually causative for this phenomenon or whether they are merely produced as a by-product. Therefore, the purpose of this study was to trigger mitochondrial ROS production in fission yeast by overexpression of either wildtype Adx (Adx-WT) or of several activated Adx mutants and to investigate its consequences. It was found that strong expression of either Adx-WT or Adx-S112W did not produce any ROS, while Adx-D113Y caused a twofold and Adx1-108 a threefold increase in ROS formation as compared to basal levels. However, no typical apoptotic markers or decreased viability could be observed in these strains. Since we previously observed that an increase in mitochondrial ROS formation of about 60% above basal levels is sufficient to strongly induce apoptosis in mammalian cells, we conclude that S. pombe is either very robust to mitochondrial ROS production or does not undergo apoptotic cell death in response to mitochondrial ROS at all.
Derouet-Hümbert
Evi
E
Department of Biochemistry, Building A 2-4, Saarland University, 66041, Saarbrucken, Germany.
Drăgan
Călin-Aurel
CA
Hakki
Tarek
T
Bureik
Matthias
M
D000325:Adrenodoxin / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers; D002417:Cattle; D003350:Cortodoxone / Q000378:metabolism; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D050296:Microbial Viability / Q000187:drug effects; D050505:Mutant Proteins / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D013252:Steroid 11-beta-Hydroxylase / Q000378:metabolism; D013997:Time Factors
D015415:Biomarkers; D050505:Mutant Proteins; D017382:Reactive Oxygen Species; D000325:Adrenodoxin; D006861:Hydrogen Peroxide; D013252:Steroid 11-beta-Hydroxylase; D003350:Cortodoxone; D006854:Hydrocortisone