Pentoxifylline inhibits Vgamma9/Vdelta2 T lymphocyte activation of patients with active Behçets disease in vitro.
The aim of this study is to evaluate the in vitro effect of pentoxifylline (PTX) on T Vgamma9/Vdelta2 lymphocyte function in Behçets disease (BD). We investigated the effect of PTX on Vgamma9/Vdelta2 T cell expansion and expression of TNFRII receptor and perforin content before and after PTX addition by means of FACS analysis lymphocyte cultures from patients with active and inactive BD and healthy subjects. The addition of PTX at a concentration of 1 mg/ml determined a significant inhibition of cell expansion, a down regulation of TNF receptor expression and inhibited the PMA-induced degranulation of perforin. Taken together these data indicate that PTX is capable of interfering with Vgamma9/Vdelta2 T cell function in BD, and although cell culture models cannot reliably predict all of the potential effects of the drug in vivo, our results encourage the possibility that this drug may find use in a range of immunological disorder characterized by dysregulated cell-mediated immunity.
Accardo-Palumbo
A
A
Department of Internal Medicine, Division of Rheumatology, University of Palermo, Italy.
Ferrante
A
A
Ciccia
F
F
Cadelo
M
M
Giardina
A R
AR
Impastato
R
R
Triolo
G
G
D000328:Adult; D001528:Behcet Syndrome / Q000097:blood / Q000276:immunology*; D003593:Cytoplasm / Q000187:drug effects / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology; D008297:Male; D010431:Pentoxifylline / Q000494:pharmacology*; D054353:Perforin / Q000378:metabolism; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000096:biosynthesis*; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
D010726:Phosphodiesterase Inhibitors; D016692:Receptors, Antigen, T-Cell, gamma-delta; D047889:Receptors, Tumor Necrosis Factor, Type II; D054353:Perforin; D010431:Pentoxifylline