Morphologic, ultrastructural, and biochemical identification of apoptosis in vitrified-warmed mouse ovarian tissue.
OBJECTIVE
To evaluate the incidence of apoptosis after vitrification warming of mouse ovaries.


METHODS
Experimental study.


METHODS
University-based research laboratory.


METHODS
Twelve- to 14-day-old National Medical Research Institute female mice.


METHODS
Vitrification of mouse ovaries using ethylene glycol.


METHODS
Follicle viability assessment by trypan blue testing, morphologic examination by hematoxylin-eosin staining and transmission electron microscopy, apoptosis assessment using the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling method and DNA laddering technique.


RESULTS
No statistically significant difference in follicle viability was observed between vitrified and nonvitrified ovaries. On transmission electron microscopy, vitrified ovaries showed a well-preserved ultrastructure. No sign of apoptosis was observed morphologically or by transferase-mediated deoxyuridine triphosphate nick end-labeling technique in either fresh or vitrified-warmed mouse ovaries. Despite the presence of a laddering pattern of DNA in control induced thymic tissue, no similar pattern was observed in fresh or vitrified-warmed ovaries.


CONCLUSIONS
The data suggest that the vitrification technique does not induce apoptosis in mouse ovarian tissue investigated just after warming.
Mazoochi
Tahere
T
Department of Anatomy, Tarbiat Modares University, Tehran, Iran.
Salehnia
Mojdeh
M
Valojerdi
Mojtaba Rezazadeh
MR
Mowla
S Javad
SJ
D000818:Animals; D017209:Apoptosis; D002470:Cell Survival; D015925:Cryopreservation; D005260:Female; D051379:Mice; D006080:Ovarian Follicle / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure*