Heat acclimation and HSP-72 expression in exercising humans.
The purpose of this research was to determine the effect of a 7-day heat acclimation protocol on HSP-72 expression in human skeletal muscle, and to examine the relationships between molecular and physiological markers of heat acclimation. Ten recreationally active male subjects (age = 23.3 +/- 2.81 yrs, VO(2peak) = 3.85 +/- 0.11 L . min (-1)) completed a 7-day heat acclimation protocol consisting of cycling at 75 % of VO(2peak) in a hot environment (39.5 degrees C, 27 % RH). Muscle biopsies were obtained on days 1 (HTT1) and 7 (HTT2) prior to, 6 h post, and 24 h postexercise to measure HSP-72 protein via SDS-PAGE and silver staining. Core rectal temperatures (T(C)), intramuscular temperatures (T(IM)), skin temperatures (T(SK)), heart rate (HR), oxygen uptake (VO(2)), sweat rate (SR), and plasma cortisol were measured. TC, HR, and plasma cortisol were significantly lower in HTT2 than HTT1 (p < or = 0.05). No significant differences were seen for VO(2), TIM, TSK, or SR when comparing HTT2 with HTT1 (p < or = 0.05). No significant time or day x time interactions were detected for HSP-72 expression (24.48 +/- 2.55 vs. 25.04 +/- 1.43 ng/microg protein for HTT1 and HTT2, respectively, p < or = 0.05). Evidence of heat acclimation was seen at the physiological level; however, no evidence of enhanced thermotolerance at the cellular level was indicated by HSP-72 expression.
Watkins
A M
AM
Department of Kinesiology, TCU, Fort Worth, TX 76129, USA.
Cheek
D J
DJ
Harvey
A E
AE
Blair
K E
KE
Mitchell
J B
JB
D000222:Adaptation, Physiological; D000328:Adult; D001706:Biopsy; D001831:Body Temperature / Q000502:physiology; D016552:Ergometry; D015444:Exercise / Q000502:physiology*; D050884:HSP72 Heat-Shock Proteins / Q000378:metabolism*; D006339:Heart Rate / Q000502:physiology; D006358:Hot Temperature; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
D050884:HSP72 Heat-Shock Proteins; D006854:Hydrocortisone