Flexible automated approach for quantitative liquid handling of complex biological samples.
A fully automated protein precipitation technique for biological sample preparation has been developed for the quantitation of drugs in various biological matrixes. All liquid handling during sample preparation was automated using a Hamilton MicroLab Star Robotic workstation, which included the preparation of standards and controls from a Watson laboratory information management system generated work list, shaking of 96-well plates, and vacuum application. Processing time is less than 30 s per sample or approximately 45 min per 96-well plate, which is then immediately ready for injection onto an LC-MS/MS system. An overview of the process workflow is discussed, including the software development. Validation data are also provided, including specific liquid class data as well as comparative data of automated vs manual preparation using both quality controls and actual sample data. The efficiencies gained from this automated approach are described.
Palandra
Joe
J
Bioanalytical Research, Department of Pharmacokinetics, Dynamics & Metabolism, Pfizer Global Research and Development, 2800 Plymouth Road, Ann Arbor, Michigan 48105, USA.
Weller
David
D
Hudson
Gary
G
Li
Jeff
J
Osgood
Sarah
S
Hudson
Emily
E
Zhong
Min
M
Buchholz
Lisa
L
Cohen
Lucinda H
LH
D019840:2-Propanol / Q000032:analysis; D000097:Acetonitriles / Q000032:analysis; D000818:Animals; D001331:Automation; D002853:Chromatography, Liquid / Q000379:methods / Q000592:standards; D004121:Dimethyl Sulfoxide / Q000032:analysis; D010949:Plasma / Q000737:chemistry; D011786:Quality Control; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012984:Software; D013048:Specimen Handling; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards; D014867:Water / Q000032:analysis
D000097:Acetonitriles; D014867:Water; D019840:2-Propanol; D004121:Dimethyl Sulfoxide; C032159:acetonitrile