A sensitive spectrophotometric method for the determination of sulfonamides in pharmaceutical preparations.
A new, simple and sensitive spectrophotometric method for the determination of some sulfonamide drugs has been developed. The method is based on the diazotization of sulfacetamide, sulfadiazine, sulfaguanidine, sulfamerazine, sulfamethazine, sulfamethoxazole, and their coupling with 8-hydroxyquinoline in alkaline media to yield red coloured products with absorption maxima at 500 nm. Beer's law is obeyed from 0.1-7.0 microg mL-1. The limits of quantification and limits of detection were 0.11-0.18 and 0.03-0.05 microg mL-1, respectively. Intraday precision (RSD 0.1-0.5%) and accuracy (recovery 97.3--100.8%) of the developed method were evaluated. No interference was observed from common adjuvants. The method has been successfully applied to the assay of sulpha drug in pharmaceutical formulations.
Nagaraja
Padmarajaiah
P
Department of Studies in Chemistry, University of Mysore, Manasagangothri Mysore-570006, India. nagarajap58@yahoo.co.in
Naik
Shailendra D
SD
Shrestha
Ashwinee Kumar
AK
Shivakumar
Anantharaman
A
D001391:Azo Compounds / Q000737:chemistry; D003116:Color; D005079:Excipients / Q000737:chemistry; D015394:Molecular Structure; D015125:Oxyquinoline / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013449:Sulfonamides / Q000032:analysis* / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000379:methods
D001391:Azo Compounds; D005079:Excipients; D004364:Pharmaceutical Preparations; D013449:Sulfonamides; D015125:Oxyquinoline