Application of ultrasound in preparing pathological sections to reduce processing time.
It has been found experimentally that application of sub-mega and low megahertz ultrasound (US) of spatial and temporal averaged intensity /(sata) up to 10 W/cm(2) during the process of preparing pathological sections of the mouse tissue has shortened the processing time from 12h (without US) to less than half an hour (with US). The experiment has also showed that the processing time reached the shortest for ultrasound f=200 kHz among the frequencies of 200 kHz, 400 kHz, 600 kHz, 800 kHz and 1 MHz used in this study. It has been proposed that ultrasound inducing non-inertial cavitation enhanced the permeability of cell membrane to liquid. Thus tissue fixation and dehydration were speeded up by application of US.
Chen
Qian
Q
Key Laboratory of Modern Acoustics, Ministry of Education and Institute of Acoustics, Nanjing University, Nanjing 210093, People's Republic of China.
Zou
Xinye
X
Cheng
Jianchun
J
Wu
Junru
J
D000818:Animals; D016591:Histocytological Preparation Techniques / Q000379:methods*; D007668:Kidney / Q000166:cytology*; D051379:Mice; D008813:Mice, Inbred ICR; D013010:Sonication; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors