title: Centrifugal seeding increases seeding efficiency and cellular distribution of bone marrow stromal cells in porous biodegradable scaffolds.
abstract: Bone marrow stromal cells (MSCs) are a promising cell source for a variety of tissue engineering applications, given their ready availability and ability to differentiate into multiple cell lineages. MSCs have been successfully used to create neotissue for cardiovascular, urological, and orthopedic reconstructive surgical procedures in preclinical studies. The ability to optimize seeding techniques of MSCs onto tissue engineering scaffolds and the ability to control neotissue formation in vitro will be important for the rational design of future tissue engineering applications using MSCs. In this study we investigated the effect of centrifugal force on seeding MSCs into a biodegradable polyester scaffold. MSCs were isolated and seeded onto porous scaffold sections composed of nonwoven polyglycolic acid mesh coated with poly(L-lactide-co-epsilon-caprolactone). Compared to standard static seeding techniques, centrifugal seeding increased the seeding efficiency by 38% (p < 0.007) and significantly improved cellular distribution throughout the scaffold. Overall, centrifugal seeding of MSCs enhances seeding efficiency and improves cellular penetration into scaffolds, making it a potentially useful technique for manipulating neotissue formation by MSCs for tissue engineering applications.
authors: Department of Surgery, Yale University School of Medicine, New Haven, Connecticut 06520, USA. jason.roh@yale.edu
authors: Jason D
authors: JD
authors: Roh
authors: Gregory N
authors: GN
authors: Nelson
authors: Brooks V
authors: BV
authors: Udelsman
authors: Matthew P
authors: MP
authors: Brennan
authors: Britt
authors: B
authors: Lockhart
authors: Peter M
authors: PM
authors: Fong
authors: Reynold I
authors: RI
authors: Lopez-Soler
authors: W Mark
authors: WM
authors: Saltzman
authors: Christopher K
authors: CK
authors: Breuer
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D002498:Centrifugation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004247:DNA / Q000032:analysis; D004146:Dioxanes / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism; D006909:Hydroxyproline / Q000032:analysis; D007150:Immunohistochemistry; D011091:Polyesters / Q000737:chemistry; D016062:Porosity; D012756:Sheep; D017154:Stromal Cells / Q000378:metabolism* / Q000502:physiology; D013526:Surgical Mesh; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D004146:Dioxanes; D011091:Polyesters; C062968:lactide-caprolactone copolymer; D004247:DNA; C025223:1,4-dioxane; D006909:Hydroxyproline