High speed and reproducible analysis of nitrosamines by capillary electrophoresis with a sulfonated capillary.
Recently environmental control is regarded as important for good human health conditions, and toxic substances, including carcinogens and endocrine disruptors should be eliminated from our living environment. Hence easy quantitative methods are expected for a high level of environmental control. Our previous paper describes an easy quantitative analysis of nitrosamines (NAs) by capillary electrophoresis with an untreated fused silica capillary installed in an ordinary apparatus. In this paper, utilizing a novel type capillary column having sulfonated inner wall was investigated for improvements of separation performance and reproducibility. A sulfonated capillary causes fast and stabile electroosmotic flow because its inner wall is strongly negative charged. On a performance comparison of a sulfonated capillary with an untreated fused silica, analysis time reduction of c.a. forty percent was achieved, and relative standard deviations of migration times and peak responses were less than one third. In addition sample concentrations giving detection and quantitation limits were also reduced to a half.
Taga
Atsushi
A
School of Pharmacy, Kinki University, Kowakae, Higashi-osaka, Japan. punk@phar.kindai.ac.jp
Nishi
Tomoko
T
Honda
Yoshitaka
Y
Sato
Atsushi
A
Terashima
Hiroyuki
H
Suzuki
Kentaro
K
Kodama
Shuji
S
Boki
Keito
K
D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D009602:Nitrosamines / Q000032:analysis*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry; D013997:Time Factors
D009602:Nitrosamines; D013451:Sulfonic Acids; D012822:Silicon Dioxide