title: A novel flavonoid from Lespedeza virgata (Thunb.) DC.: structural elucidation and antioxidative activity.
abstract: Bioactivity guided fractionation of the ethanolic extract of the whole plants of Lespedeza virgata (Thunb.) DC. resulted in the isolation of a novel flavonoid (1) along with five known compounds 2-6. The molecular and structural formula as well as the stereochemistry of compound 1 were determined using data obtained from (1)H and (13)C NMR spectra, DEPT135 and by 2D HSQC, HMBC, (1)H-(1)H correlated spectroscopy ((1)H-(1)H COSY), and nuclear overhauser effect spectroscopy (NOESY) experiments. The superoxide anion scavenging activities of all isolated compounds were evaluated by the hypoxanthine nitro blue tetrazolium and ESR methods and the new compound 1 showed the strongest antioxidative activity 95.79% (IC(50)=0.14 mg/ml).
authors: State Key Laboratory for SCUSS, Institute of Chemistry, The Chinese Academy of Sciences, Beijing, PR China.
authors: Li
authors: L
authors: Tan
authors: Xiu-Feng
authors: XF
authors: Zhang
authors: Bao-Zhen
authors: BZ
authors: Yan
authors: Hai-Ming
authors: HM
authors: Shi
authors: Li-Bo
authors: LB
authors: Du
authors: Ya-Zhou
authors: YZ
authors: Zhang
authors: Lan-Fen
authors: LF
authors: Wang
authors: Ya-Lin
authors: YL
authors: Tang
authors: Yang
authors: Y
authors: Liu
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D004578:Electron Spin Resonance Spectroscopy; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D020128:Inhibitory Concentration 50; D029884:Lespedeza / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D012997:Solvents / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D012997:Solvents; D013481:Superoxides; D000431:Ethanol