[Study on molecular cytogenetic abnormalities in multiple myeloma].
OBJECTIVE
To explore the molecular cytogenetic abnormalities in multiple myeloma (MM).


METHODS
Bone marrow plasma cells from 23 previously untreated MM patients were purified by CD138 McAb magnetic cell sorting system, and a panel of probes for interphase fluorescence in situ hybridization were used to detect the 13q14 deletion, p53 deletion and IgH gene translocation in the sorted MM cells.


RESULTS
Among 23 MM patients, 13q14 deletion was observed in 10 (43.5%) cases, with the positive rate of 13q14 deleted cells ranged from 79% to 96%; 14q32 translocation was observed in 11 (47.8%) cases; 13q14 deletion and 14q32 translocation were simultaneously observed in 7 (30.4%) cases; and p53 deletion was observed in none of the 23 cases.


CONCLUSIONS
The frequency of 13q14 deletion and IgH gene translocation in multiple myeloma are high; and the relationship between 13q14 deletion, IgH gene translocation and prognosis is worth further investigating.
Liu
Shu-Yan
SY
Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, China.
Li
Jian-Yong
JY
Chen
Li-Juan
LJ
Huang
Jin-Wen
JW
Pan
Jin-Lan
JL
Qiu
Hai-Rong
HR
Shen
Yun-Feng
YF
Xu
Wei
W
Xue
Yong-Quan
YQ
D000368:Aged; D002869:Chromosome Aberrations; D005260:Female; D017353:Gene Deletion; D015321:Gene Rearrangement; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics*; D010950:Plasma Cells
D007143:Immunoglobulin Heavy Chains