Effects of chlorine injury, starvation, and Colitag(TM) enrichment on sandwich enzyme-linked immunosorbent assay (sELISA) detection of Escherichia coli O157:H7.
The validity of ELISA for detection of E. coli O157:H7 under many conditions is not proven. In this work, sELISA was able to detect bacteria after sub-lethal chlorine exposure and after seven days of starvation with little to no change in limit of detection and fluorescence signal as long as chlorine was not present in the sample or was neutralized by sodium thiosulfate. After Colitag enrichment, sELISA detected approximately 3 colony forming units/ml of originally added E. coli O157:H7. Thus, the present sELISA is valid for detection of E. coli O157:H7 in water sources, although sample matrices may interfere with assay.
Nyquist-Battie
Cynthia
C
Department of Public Health, University of North Florida, 4569 St John's Bluff Road, Jacksonville, FL 32224, USA. cnyquist@unf.edu
Freeman
Laura
L
Soberano
John-Paul
JP
Lund
Deanna
D
Kearns
Elizabeth
E
Lim
Daniel V
DV
D001431:Bacteriological Techniques; D002713:Chlorine / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019453:Escherichia coli O157 / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D014871:Water Microbiology
D002713:Chlorine