Identification of culturable stream water bacteria from urban, agricultural, and forested watersheds using 16S rRNA gene sequencing.
Bacteria present in water samples taken on a weekly basis, from June 2004 through June 2005, from three streams, were cultured on Coliscan Easygel agar plates. Colonies representative of a variety of colors and morphologies were subjected to amplification and sequencing of a 1000-1100 nt portion of the 16S rRNA gene. A total of 528 colonies were sequenced; these categorized into 26 genera and 78 species. Of 175 dark blue/purple colonies presumed to be E. coli, sequence analysis indicated that 45 (25%) were actually other genera. For the urban stream Gwynns Falls Gwynns Run, E. coli was the most common genus/species encountered, followed by Klebsiella and Aeromonas. For Pond Branch, a stream located in a forested watershed, it was Serratia, followed by Yersinia and Aeromonas. For McDonogh (MCDN), a stream associated with Zea mays (corn) row crop agriculture, E. coli was the most frequently isolated genus/species, followed by Aeromonas and Enterobacter. ERIC-PCR genotyping of isolates from the most prevalent genera/species, indicated a high degree of diversity within-stream for E. coli and K. pneumoniae. Conversely, genotyping of Y. enterocolitica isolates indicated that some were shared between different streams.
Belt
Kenneth T
KT
USDA Forest Service, UMBC CUERE/BES LTER, Baltimore, MD 21227, USA.
Hohn
Christina
C
Gbakima
Aiah
A
Higgins
James A
JA
D000383:Agriculture; D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002947:Cities; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000235:genetics; D004784:Environmental Monitoring; D014644:Genetic Variation; D008396:Maryland; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017423:Sequence Analysis, RNA; D014197:Trees; D014871:Water Microbiology; D014873:Water Pollutants / Q000145:classification* / Q000302:isolation & purification; D014881:Water Supply
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014873:Water Pollutants