title: Expression and regulation of versican in neural precursor cells and their lineages.
abstract: OBJECTIVE
  To have a better understanding of the expression and regulation of versican isoforms in neural precursor cells (NPC) and oligodendrogliogenesis.
  
  
  METHODS
  By immunocytochemistry, RT-PCR, and real-time PCR, we examined the temporal expression of versican in NPC isolated from embryonic d 16 rats as well as in oligodendrocyte (OL) lineage cells induced to differentiate from NPC, which mimicked the oligodendrogliogenesis in vivo.
  
  
  RESULTS
  We found that versican was constitutively expressed in NPC and their lineage cells, including neurons, astrocytes, and OL. In addition, 2 versican isoforms, V1/V0 and V2, were found to express at low levels in NPC, but at significantly higher levels in OL lineage cells. The peak expression of versican V2 was found at the oligodendrocyte precursor cell stage. Furthermore, the treatment of 2 pro-inflammatory cytokines, TNF-alpha and IFN-gamma, enhanced the transcription of versican V2 in NPC in a dose-dependent manner, but showed no effect on V1/V0 expression.
  
  
  CONCLUSIONS
  Taken together, our results demonstrate that versican, particularly the inhibitory V2 isoform, is increasingly expressed in OL lineage cells induced to differentiate from NPC. An increase in versican V2 expression after cytokine stimulation implies the interplay between the injury-induced upregulation of inflammatory cytokines and chondroitin sulfate proteoglycan-mediated inhibition of axonal regeneration after central nervous system injury.
authors: Department of Neurobiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors: Wen-Li
authors: WL
authors: Gu
authors: Sai-Li
authors: SL
authors: Fu
authors: Yan-Xia
authors: YX
authors: Wang
authors: Ying
authors: Y
authors: Li
authors: Xiao-Fei
authors: XF
authors: Wang
authors: Xiao-Ming
authors: XM
authors: Xu
authors: Pei-Hua
authors: PH
authors: Lu
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013116:Spinal Cord / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D053675:Versicans / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D053675:Versicans; D007371:Interferon-gamma