Renoprotective effect of erdosteine in rats against gentamicin nephrotoxicity: a comparison of 99mTc-DMSA uptake with biochemical studies.
Erdosteine is a mucolytic agent having antioxidant properties through its active metabolites in acute injuries induced by pharmacological drugs. This study was designed to investigate the renoprotective potential of Erdosteine against gentamicin (GM)-induced renal dysfunction by using Technetium-99 m dimercaptosuccinic acid (Tc-99 m DMSA) uptake and scintigraphy in rats. For this purpose, male Wistar rats were randomly allotted into one of the four experimental groups: Control, Erdosteine, GM, and GM + Erdosteine groups. GM and GM + Erdosteine groups received 100 mg/kg GM intramuscularly for 6 days. In addition, Erdosteine and GM + Erdosteine groups received 50 mg/kg Erdosteine orally for 6 days. Renal function tests were assessed by serum blood urea nitrogen (BUN), creatinine levels, as well as scintigraphic and tissue radioactivity measurements with Tc-99 m DMSA. Renal oxidative damage was determined by renal malondialdehyde (MDA) levels, by antioxidant enzyme activities; superoxide dismutase (SOD) and catalase (CAT) and activities of oxidant enzymes; xanthine oxidase (XO) and myeloperoxidase (MPO). GM administration resulted in marked renal lipid peroxidation, increased XO and MPO activities and decreased antioxidant enzyme activities. GM + Erdosteine group significantly had lower MDA levels, higher SOD and CAT activities and lower XO and MPO activities, when compared to GM. Also GM + Erdosteine had lower levels of serum BUN, creatinine and higher renal tissue Tc-99 m DMSA uptake and radioactivity with respect to GM. In conclusion, our results supported a protective role of Erdosteine in nephrotoxicity associated with GM treatment.
Cabuk
Mehmet
M
Department of Nuclear Medicine, Faculty of Medicine, Zonguldak Karaelmas University, 67600 Kozlu, 44069 Zonguldak, Turkey. mehmetcabuk87@yahoo.com
Gurel
Ahmet
A
Sen
Feyza
F
Demircan
Nejat
N
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D005839:Gentamicins / Q000633:toxicity*; D007668:Kidney / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D011877:Radionuclide Imaging; D051381:Rats; D017208:Rats, Wistar; D019783:Technetium Tc 99m Dimercaptosuccinic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000494:pharmacology; D013864:Thioglycolates / Q000494:pharmacology*; D013876:Thiophenes / Q000494:pharmacology*
D000975:Antioxidants; D005839:Gentamicins; D016877:Oxidants; D020011:Protective Agents; D013864:Thioglycolates; D013876:Thiophenes; D019783:Technetium Tc 99m Dimercaptosuccinic Acid; D008315:Malondialdehyde; C048498:erdosteine; D003404:Creatinine