Evaluation of cytomegalovirus (CMV) DNA quantification in dried blood spots: retrospective study of CMV congenital infection.
We compared two protocols for extracting DNA from dried blood spots for cytomegalovirus (CMV) DNA detection and quantification by real-time PCR. Both extraction methods were reliable for the retrospective diagnosis of CMV congenital infection. Quantification of CMV DNA was valuable after normalization of viral loads with albumin gene PCR amplification results.
Vauloup-Fellous
Christelle
C
Service de Microbiologie Immunologie Biologique, Assistance Publique-Hôpitaux de Paris, Hôpital Antoine Béclère, Université Paris Sud 11, Clamart, France.
Ducroux
Aurélie
A
Couloigner
Vincent
V
Marlin
Sandrine
S
Picone
Olivier
O
Galimand
Julie
J
Loundon
Natalie
N
Denoyelle
Françoise
F
Grangeot-Keros
Liliane
L
Leruez-Ville
Marianne
M
D002675:Child, Preschool; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000151:congenital* / Q000175:diagnosis*; D004279:DNA, Viral / Q000097:blood*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction / Q000379:methods*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D019562:Viral Load
D004279:DNA, Viral