Monitoring fondaparinux with the Sonoclot.
Fondaparinux is a new anticoagulant that interacts with antithrombin III and activated coagulation factor X resulting in an inhibition of the coagulation system. It has been successful in doses of 2.5 mg for thromboprophylaxis as well as in higher therapeutic doses of 5-7.5 mg. No optimal method for monitoring the effects of fondaparinux has been proposed. The aim of the present study was to investigate whether a viscoelastic coagulation analyzer, the Sonoclot (Sienco, Denver, Colorado, USA), could be used for in-vitro monitoring of fondaparinux. Different concentrations of fondaparinux were added in vitro to whole blood taken from eight volunteers. The blood samples mixed with the various amounts of fondaparinux were analyzed using the Sonoclot. The whole-blood activated partial thromboplastin time with the Hemochron Jr (ITC, Edison, New Jersey, USA) was used as the reference coagulation analysis. All analyses were started expeditiously, within 30 s from sampling, and were performed at 37 degrees C. The values of the Sonoclot parameter clot rate, which measures the rate of fibrin formation, fibrin polymerization and platelet-fibrin interactions, were significantly correlated to increasing concentrations of fondaparinux (R = -0.90). The Sonoclot parameters of activated coagulation time, time to peak and clot retraction had weaker, but still significant, correlations to fondaparinux concentrations. At prophylactic doses (0.38 microg/ml blood) the clot rate decreased 15% compared with the initial unanticoagulated value, whereas at therapeutic doses (1.53 microg/ml blood) there was a 27% decrease. In conclusion, the Sonoclot parameter clot rate could be of clinical value to individualize the fondaparinux dosage, especially the higher, therapeutic, dosages.
Nilsson
Caroline U
CU
Central Hospital Halmstad, Halmstad, Halland, Sweden.
Engström
Martin
M
D000925:Anticoagulants / Q000097:blood* / Q000627:therapeutic use; D001780:Blood Coagulation Tests / Q000379:methods*; D003018:Clot Retraction / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D000077425:Fondaparinux; D006801:Humans; D007700:Kinetics; D011134:Polysaccharides / Q000097:blood* / Q000627:therapeutic use; D014465:Ultrasonics; D014914:Whole Blood Coagulation Time / Q000295:instrumentation* / Q000379:methods* / Q000706:statistics & numerical data
D000925:Anticoagulants; D011134:Polysaccharides; D000077425:Fondaparinux