title: Skin-derived dendritic cells can mediate deletional tolerance of class I-restricted self-reactive T cells.
abstract: Skin-draining lymph nodes contain a number of dendritic cell (DC) subsets of different origins. Some of these are migratory, such as the skin-derived epidermal Langerhans cells and a separate dermal DC subset, whereas others are lymphoid resident in nature, such as the CD8+ DCs found throughout the lymphoid tissues. In this study, we examine the DC subset presentation of skin-derived self-Ag by migratory and lymphoid-resident DCs, both in the steady state and under conditions of local skin infection. We show that presentation of self-Ag is confined to skin-derived migrating DCs in both settings. Steady state presentation resulted in deletional T cell tolerance despite these DCs expressing a relatively mature phenotype as measured by traditional markers such as the level of MHC class II and CD86 expression. Thus, self-Ag can be carried to the draining lymph nodes by skin-derived DCs and there presented by these same cells for tolerization of the circulating T cell pool.
authors: Department of Microbiology and Immunology, University of Melbourne, Parkville, Australia.
authors: Jason
authors: J
authors: Waithman
authors: Rhys S
authors: RS
authors: Allan
authors: Hiroshi
authors: H
authors: Kosaka
authors: Hiroaki
authors: H
authors: Azukizawa
authors: Ken
authors: K
authors: Shortman
authors: Manfred B
authors: MB
authors: Lutz
authors: William R
authors: WR
authors: Heath
authors: Francis R
authors: FR
authors: Carbone
authors: Gabrielle T
authors: GT
authors: Belz
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D000941:Antigens / Q000276:immunology; D015551:Autoimmunity / Q000276:immunology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D007108:Immune Tolerance / Q000276:immunology*; D007801:Langerhans Cells / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000276:immunology; D012867:Skin / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D000941:Antigens; D015395:Histocompatibility Antigens Class I; D010047:Ovalbumin