Fast conventional Fmoc solid-phase peptide synthesis with HCTU.
1H-Benzotriazolium 1-[bis(dimethyl-amino)methylene]-5-chloro-hexafluorophosphate (1-),3-oxide (HCTU) is a nontoxic, nonirritating and noncorrosive coupling reagent. Seven biologically active peptides (GHRP-6, (65-74)ACP, oxytocin, G-LHRH, C-peptide, hAmylin(1-37), and beta-amyloid(1-42)) were synthesized with reaction times reduced to deprotection times of 3 min or less and coupling times of 5 min or less using HCTU as the coupling reagent. Expensive coupling reagents or special techniques were not used. Total peptide synthesis times were dramatically reduced by as much as 42.5 h (1.8 days) without reducing the crude peptide purities. It was shown that HCTU can be used as an affordable, efficient coupling reagent for fast Fmoc solid-phase peptide synthesis.
Hood
Christina A
CA
Department of Chemistry, Protein Technologies Inc., Tucson, Arizona, 4675 South Coach Drive, Tucson, Arizona 85714, USA.
Fuentes
German
G
Patel
Hirendra
H
Page
Karen
K
Menakuru
Mahendra
M
Park
Jae H
JH
D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry*; D016229:Amyloid beta-Peptides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry; D006801:Humans; D058228:Islet Amyloid Polypeptide; D008969:Molecular Sequence Data; D010121:Oxytocin / Q000737:chemistry; D010452:Peptide Biosynthesis; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D013997:Time Factors; D014230:Triazoles / Q000737:chemistry*
C472083:1-(bis(dimethylamino)methylene)-1H-benzotriazolium hexafluorophosphate 3-oxide; C527961:1H-benzotriazolium-1-(bis(dimethyl-amino)methylene)-5-chloro-hexafluorophosphate (1-),3-oxide; D000682:Amyloid; D016229:Amyloid beta-Peptides; D058228:Islet Amyloid Polypeptide; D010455:Peptides; D014230:Triazoles; D007987:Gonadotropin-Releasing Hormone; D010121:Oxytocin