Apoptosis induction by Maackia amurensis agglutinin in childhood acute lymphoblastic leukemic cells.
Malignant transformation is known to be associated with changes in cell surface carbohydrate-architecture, which can be detected by lectins. In the present study, Maackia amurensis agglutinin (MAA), specific for NeuNAcalpha(2-->3)Gal/GalNAc showed strong binding with lymphoblasts of children having acute lymphoblastic leukemia (ALL) as compared to cells from children with non-hematological disorders ("Controls"). MAA recognized a 66 kDa sialoglycoprotein present in membrane fraction of ALL cells. Moreover, MAA induced apoptosis in ALL cells was found to be reduced significantly in presence of GM2/IgG(MAA). Thus, MAA has a potential to be used as diagnostic and therapeutic agent in case of childhood-ALL.
Kapoor
Sarika
S
Department of Pediatrics, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.
Marwaha
Ram
R
Majumdar
Siddhartha
S
Ghosh
Sujata
S
D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D009439:Neuraminidase / Q000737:chemistry; D010835:Phytohemagglutinins / Q000737:chemistry / Q000494:pharmacology*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism / Q000473:pathology*
D006023:Glycoproteins; D010835:Phytohemagglutinins; C100156:leukoagglutinins, plants; C100877:sialidase L; D009439:Neuraminidase