title: T cells specific to hapten carrier but not to carrier alone assist in the production of anti-hapten and anti-carrier antibodies.
abstract: We examined the immune response of Balb/c mice to antigens prepared by conjugating 2-phenyloxazolone (phOx) to a foreign protein, ovalbumin (OVA), or a self-protein, mouse serum albumin (MSA), in order to study how these chemical modifications would affect immune recognition. We found that anti-OVA antibodies and CD4(+) T cells produced by OVA immunization reacted with OVA as well as with phOx-OVA. Anti-phOx antibodies were produced by phOx-OVA immunization and, interestingly, T cells from these mice reacted only with phOx-OVA but not with the intact OVA. These results suggested that the classical model of hapten-carrier immunization, in which B cells specific to hapten are activated with assistance from T cells specific to a carrier protein, might not be a major route for production of anti-hapten antibodies in hapten-carrier immunization. Furthermore, phOx-MSA immunization induced production of anti-phOx antibodies, which could not be accounted for in terms of the assistance of carrier-specific T cells because of the absence of MSA-specific T cells. Therefore, we proposed a new model in which anti-hapten B cells are assisted by T cells specific to the haptenated carrier.
authors: Division of Structural Immunology, Research Institute for Biological Sciences, Tokyo University of Science, Yamazaki 2669, Noda, Chiba 278-0022, Japan.
authors: Takeyuki
authors: T
authors: Shimizu
authors: Yuko
authors: Y
authors: Osaka
authors: Chihiro
authors: C
authors: Banri-Koike
authors: Maiko
authors: M
authors: Yoshida
authors: Kanako
authors: K
authors: Endo
authors: Koji
authors: K
authors: Furukawa
authors: Masayuki
authors: M
authors: Oda
authors: Akikazu
authors: A
authors: Murakami
authors: Shuhei
authors: S
authors: Ogawa
authors: Ryo
authors: R
authors: Abe
authors: Takachika
authors: T
authors: Azuma
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D006241:Haptens / Q000276:immunology*; D007114:Immunization; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D010081:Oxazolone / Q000031:analogs & derivatives* / Q000276:immunology
chemical_list: D000906:Antibodies; D000941:Antigens; D006241:Haptens; C018361:2-phenyloxazolone; D010081:Oxazolone; D010047:Ovalbumin