Protein secondary structure analyses from circular dichroism spectroscopy: methods and reference databases.
Circular dichroism (CD) spectroscopy has been a valuable method for the analysis of protein secondary structures for many years. With the advent of synchrotron radiation circular dichroism (SRCD) and improvements in instrumentation for conventional CD, lower wavelength data are obtainable and the information content of the spectra increased. In addition, new computation and bioinformatics methods have been developed and new reference databases have been created, which greatly improve and facilitate the analyses of CD spectra. This article discusses recent developments in the analysis of protein secondary structures, including features of the DICHROWEB analysis webserver.
Whitmore
Lee
L
Department of Crystallography, Birkbeck College, University of London, London WC1E 7HX, UK.
Wallace
B A
BA
D002942:Circular Dichroism / Q000379:methods / Q000639:trends; D030562:Databases, Protein / Q000592:standards; D015394:Molecular Structure; D017433:Protein Structure, Secondary; D012015:Reference Standards; D012984:Software