title: Highly specific novel method for isolation and purification of lupus anti-DNA antibody via oligo-(dT) magnetic beads.
abstract: A novel method for isolation and purification of anti-ssDNA antibodies from human sera is developed. The process involves: antibody purification based on their affinity for single-stranded sequence of thymidines and removal of remaining components via protein G coated magnetic beads, with high affinity for only IgG subclass. The high degree of purity and molecular weights of healthy versus lupus anti-ssDNA antibodies were confirmed by SDS-PAGE and silver staining. Western blot confirmed IgG isotype. This novel technique allows for diagnostic purposes, structural and functional analysis of anti-DNA antibodies, and studies of their role in autoimmune diseases.
authors: Department of Biological Sciences, Charles E. Schmidt College of Science, Florida Atlantic University, Boca Raton, Florida 33451, USA. pmirjana@aol.com
authors: Mirjana
authors: M
authors: Pavlovic
authors: Ran
authors: R
authors: Chen
authors: Anna M
authors: AM
authors: Kats
authors: Michelle F
authors: MF
authors: Cavallo
authors: Seth
authors: S
authors: Saccocio
authors: Patricia
authors: P
authors: Keating
authors: James X
authors: JX
authors: Hartmann
mesh_terms: D000974:Antibodies, Antinuclear / Q000302:isolation & purification*; D000915:Antibody Affinity; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis*; D009838:Oligodeoxyribonucleotides; D012680:Sensitivity and Specificity
chemical_list: D000974:Antibodies, Antinuclear; D009838:Oligodeoxyribonucleotides; C027903:oligo (dT)