title: Rad52 multimerization is important for its nuclear localization in Saccharomyces cerevisiae.
abstract: Rad52 is essential for all homologous recombination and DNA double strand break repair events in Saccharomyces cerevisiae. This protein is multifunctional and contains several domains that allow it to interact with DNA as well as with different repair proteins. However, it has been unclear how Rad52 enters the nucleus. In the present study, we have used a combination of mutagenesis and sequence analysis to show that Rad52 from S. cerevisiae contains a single functional pat7 type NLS essential for its nuclear localization. The region containing the NLS seems only to be involved in nuclear transport as it plays no role in repair of MMS-induced DNA damage. The NLS in Rad52 is weak, as monomeric protein species that harbor this NLS are mainly located in the cytosol. In contrast, multimeric protein complexes wherein each subunit contains a single NLS(Rad52) sort efficiently to the nucleus. Based on the results we propose a model where the additive effect of multiple NLS(Rad52) sequences in a Rad52 ring-structure ensures efficient nuclear localization of Rad52.
authors: Center for Microbial Biotechnology, BioCentrum-DTU, Technical University of Denmark, Bldg. 223, DK-2800 Kgs. Lyngby, Denmark.
authors: Iben
authors: I
authors: Plate
authors: Line
authors: L
authors: Albertsen
authors: Michael
authors: M
authors: Lisby
authors: Swee C L
authors: SC
authors: Hallwyl
authors: Qi
authors: Q
authors: Feng
authors: Changhyun
authors: C
authors: Seong
authors: Rodney
authors: R
authors: Rothstein
authors: Patrick
authors: P
authors: Sung
authors: Uffe H
authors: UH
authors: Mortensen
mesh_terms: D000595:Amino Acid Sequence; D001704:Biopolymers / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D004249:DNA Damage; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals; D010957:Plasmids; D051721:Rad52 DNA Repair and Recombination Protein / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
chemical_list: D001704:Biopolymers; D019913:Nuclear Localization Signals; D051721:Rad52 DNA Repair and Recombination Protein