A reversible middle cerebral artery occlusion model using intraluminal balloon technique in monkeys.
BACKGROUND
This study was designed to develop a simple, reversible primate middle cerebral artery (MCA) occlusion (MCAO) model.


METHODS
MCAO was achieved by inserting a specially designed intraluminal balloon catheter from the external carotid artery, then to the internal carotid artery, and finally into the proximal segment of MCA using 13 adolescent monkeys (Macaca mulatta) weighing 4.25 +/- 0.23 kg. The infarction was confirmed by magnetic resonance imaging at 3, 7, and 23 hours after MCAO.


RESULTS
Local cerebral blood flow in the caudate nucleus decreased remarkably. Post-MCAO electroencephalographic monitoring showed the inhibition of electroencephalography with slow waves distinctly increasing. Neurologic evaluation demonstrated persistent deficits in the ischemic group. Neuropathologic examinations were consistent with the neurologic evaluations.


CONCLUSIONS
The results suggested that this reversible monkey model resembled a human stroke and should offer a reasonable alternative to craniotomy and the extrinsic ligation of the MCA in the laboratory investigation of focal reversible cerebral ischemia.
Gao
Huanmin
H
Department of Neurology, Second Affiliated Hospital of Qingdao University Medical College, Qingdao, China.
Liu
Yaoquan
Y
Lu
Shaoping
S
Xiang
Bo
B
Wang
Chao
C