Low-frequency stimulation of cerebellar fastigial nucleus inhibits amygdaloid kindling acquisition in Sprague-Dawley rats.
Low-frequency stimulation (LFS) of the kindling focus or the piriform cortex inhibits kindling epileptogenesis, but whether LFS of brain targets outside the limbic system has anticonvulsive actions remain unknown. The current study was designed to investigate the effect of LFS of the cerebellar fastigial nucleus (FN) on seizure progression induced by amygdaloid kindling. Stimulation at 1 Hz (15-min train of 0.1-ms pulses, 100 muA), but not at 3 Hz, in the ipsilateral FN immediately after the daily kindling stimulus (1-s train of 1-ms pulses at 60 Hz and 100-300 muA) significantly inhibited the seizure stage and afterdischarge duration in kindling acquisition. Neither 1 Hz nor 3 Hz stimulation of the contralateral FH had any significant effect. It is interesting that delaying delivery (immediately after the cessation of afterdischarge) of LFS in the ipsilateral FN accelerated the rate of kindling acquisition compared to controls. Our study suggests that LFS of targets outside the limbic system, such as the FN, may have a significant anti-epileptogenic action, and the effects of LFS depend on the frequency and timing of stimulation.
Wang
Shuang
S
Department of Pharmacology, Institute of Neuroscience, School of Medicine, Zhejiang University, Hangzhou, 310058 China.
Wu
Deng-chang
DC
Ding
Mei-ping
MP
Li
Qing
Q
Zhuge
Zhen-bing
ZB
Zhang
Shi-hong
SH
Chen
Zhong
Z
D000679:Amygdala / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D002529:Cerebellar Nuclei / Q000528:radiation effects*; D004195:Disease Models, Animal; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation / Q000379:methods*; D004569:Electroencephalography / Q000379:methods; D007839:Functional Laterality; D007696:Kindling, Neurologic / Q000502:physiology* / Q000528:radiation effects; D008297:Male; D009043:Motor Activity / Q000528:radiation effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000473:pathology / Q000628:therapy*