Tonicity-regulated gene expression.
Hypertonicity activates several different transcription factors, including TonEBP/OREBP, that in turn increase transcription of numerous genes. Hypertonicity elevates TonEBP/OREBP transcriptional activity by moving it into the nucleus, where it binds to its cognate DNA element (ORE), and by increasing its transactivational activity. This chapter presents protocols for measuring the transcriptional activity of TonEBP/OREBP and determining its subcellular localization, its binding to OREs, and activity of its transactivation domain.
Ferraris
Joan D
JD
Laboratory of Kidney and Electrolyte Metabolism, National Heart Lung Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA.
Burg
Maurice B
MB
D002460:Cell Line; D002467:Cell Nucleus / Q000502:physiology; D003593:Cytoplasm / Q000502:physiology; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D050778:NFATC Transcription Factors / Q000502:physiology*; D009997:Osmotic Pressure; D015533:Transcriptional Activation; D014162:Transfection / Q000379:methods
D050778:NFATC Transcription Factors