Pdx-1-independent differentiation of mouse embryonic stem cells into insulin-expressing cells.
To investigate whether insulin-producing cells obtained from ES cells via the nestin-positive cell-mediated method are of the pancreatic lineage, we established a pdx-1 knockout ES cell line and analyzed its differentiation into insulin-producing cells. As a result, pdx-1 knockout ES cell expressed insulin 2 gene at the final differentiated cells. Thus, our study demonstrated that pdx-1 is not essential for insulin gene expression, at least in cells differentiated from this population of nestin-expression enriched ES cells, and suggested that the insulin-producing cells derived from ES cells may be different from the pancreatic beta cells in terms of their lineage.
Takayama
I
I
Division of Stem Cell Regulation Research, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
Miyazaki
S
S
Tashiro
F
F
Fujikura
J
J
Miyazaki
J
J
Yamato
E
E
D000818:Animals; D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology*; D005091:Exons; D016678:Genome; D018398:Homeodomain Proteins / Q000235:genetics*; D007328:Insulin / Q000235:genetics / Q000378:metabolism*; D000078790:Insulin Secretion; D007381:Intermediate Filament Proteins / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000235:genetics; D064231:Nestin; D015183:Restriction Mapping; D015534:Trans-Activators / Q000172:deficiency / Q000235:genetics*
D018398:Homeodomain Proteins; D007328:Insulin; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D015534:Trans-Activators; C084010:pancreatic and duodenal homeobox 1 protein