title: Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants.
abstract: To improve the accumulation of recombinant human epidermal growth factor (hEGF) in transgenic tobacco, a highly effective vector was constructed and transformed via Agrobacterium tumefaciens. The hEGF content in transgenic tobacco was up to 0.3% of the total soluble protein. Using the Vero E6 cell expansion assay and the MTT method for cell proliferation, hEGF produced by transgenic tobacco significantly stimulated Vero E6 cell expansion and proliferation, the same as commercial hEGF products.
authors: The National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, 100871, PR China.
authors: Jie-Ying
authors: JY
authors: Bai
authors: Lin
authors: L
authors: Zeng
authors: Yuan-Lei
authors: YL
authors: Hu
authors: Yan-Fang
authors: YF
authors: Li
authors: Zhong-Ping
authors: ZP
authors: Lin
authors: Shi-Chen
authors: SC
authors: Shang
authors: Yan-Sheng
authors: YS
authors: Shi
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D049109:Cell Proliferation / Q000187:drug effects; D016009:Chi-Square Distribution; D002522:Chlorocebus aethiops; D003062:Codon; D004815:Epidermal Growth Factor / Q000378:metabolism* / Q000494:pharmacology; D005822:Genetic Vectors; D006801:Humans; D008934:Mitogens / Q000494:pharmacology; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics*; D014709:Vero Cells
chemical_list: D003062:Codon; D008934:Mitogens; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor