ADAMTS13 related markers and von Willebrand factor in plasma from patients with thrombotic microangiopathy (TMA).
The ADAMTS13 (a disintegrin and metalloprotease with a thrombospondin type I domain 13) related markers were measured in the plasma of healthy volunteers and thrombotic microangiopathy (TMA) patients including thrombotic thrombocytopenic purpura (TTP) to examine their efficacy in the diagnosis of TTP. The plasma levels of the ADAMTS13 antigen and ADAMTS13-factor XI complex were significantly lower in TMA patients with a significant decreased ADAMTS13 activity (and these patients were considered to have TTP) than in the healthy volunteers. The plasma levels of ADAMTS13 antigens closely correlated with those of ADAMTS13-factor XI complex. Autoantibody for ADAMTS 13 was also positive in almost all TTP patients. In addition, the ratio of von Willebrand factor (VWF)/ADAMTS13 activity was significantly high in TTP suggesting that this ratio might be more useful for the differential diagnosis of TTP than the ADAMTS13 assay alone. These findings suggest that ADAMTS13 related markers are useful for the diagnosis and analysis of TTP.
Kobayashi
Toshihiko
T
Department of Hematology, Mie University Graduate School of Medicine, Tsu, Japan.
Wada
Hideo
H
Nishioka
Norihiro
N
Yamamoto
Masae
M
Matsumoto
Takeshi
T
Tamaru
Tomomi
T
Nomura
Shinsuke
S
Masuya
Masahiro
M
Mori
Yoshitaka
Y
Nakatani
Kaname
K
Nishikawa
Masakatsu
M
Katayama
Naoyuki
N
Nobori
Tsutomu
T
D051722:ADAM Proteins / Q000097:blood* / Q000276:immunology; D000071120:ADAMTS13 Protein; D000293:Adolescent; D000328:Adult; D000368:Aged; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000097:blood; D001323:Autoantibodies / Q000097:blood; D015415:Biomarkers / Q000097:blood*; D005172:Factor XI / Q000276:immunology; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008833:Microcirculation / Q000503:physiopathology; D008875:Middle Aged; D015203:Reproducibility of Results; D013927:Thrombosis / Q000097:blood*; D014841:von Willebrand Factor / Q000032:analysis*
D000941:Antigens; D001323:Autoantibodies; D015415:Biomarkers; D014841:von Willebrand Factor; D005172:Factor XI; D051722:ADAM Proteins; D000071120:ADAMTS13 Protein; C099604:ADAMTS13 protein, human