Histochemical examinations on cortical bone regeneration induced by thermoplastic bioresorbable plates applied to bone defects of rat calvariae.
We aimed to histologically elucidate whether bioresorbable plates (DeltaSystem) can induce cortical bone formation, which is essential for long-lasting bone augmentation. Standardized bone defects in rat calvariae were covered with a convexly-shaped DeltaSystem plate, and then processed for histological observations. At 1 week, alkaline phosphatase-positive osteoblasts were seen in the newly-formed bone extending from the cavity's bottom, indicating accelerated osteogenesis. A thick layer of soft connective tissue positive for periostin, a hallmark of periosteum, covered this new bone. At 2 weeks, a spongy bone had filled the cavity up to half its height. The inner layer of the soft tissue facing the spongy bone revealed abundant periostin and osteopontin, and had many tartrate-resistant acid phosphatase-positive osteoclasts. At 4 weeks, this layer had given rise to thin new bony matrices without relation to the spongy bone arising from the cavity. These bone matrices had been thickened by 8 weeks, and turned into a thick cortical bone outlining the regenerated bone at 12 weeks. Thus, our study has provided histological evidences of cortical osteogenesis when DeltaSystem plates are used for bone augmentation procedures.
Kojima
Taku
T
Division of Reconstructive Surgery for Oral and Maxillofacial Region, Niigata University Graduate School of Medical and Dental Sciences, Japan.
Freitas
Paulo H L
PH
Ubaidus
Sobhan
S
Suzuki
Akiko
A
Li
Minqi
M
Yoshizawa
Michiko
M
Oda
Kimimitsu
K
Maeda
Takeyasu
T
Kudo
Akira
A
Saito
Chikara
C
Amizuka
Norio
N
D020341:Absorbable Implants; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001860:Bone Plates; D001861:Bone Regeneration; D015815:Cell Adhesion Molecules / Q000378:metabolism; D050723:Fractures, Bone / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D008297:Male; D010006:Osteoblasts / Q000378:metabolism / Q000473:pathology*; D010010:Osteoclasts / Q000378:metabolism / Q000473:pathology*; D010012:Osteogenesis; D053495:Osteopontin / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000293:injuries / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors
D015815:Cell Adhesion Molecules; C512836:Postn protein, rat; C506897:Spp1 protein, rat; D053495:Osteopontin; D000469:Alkaline Phosphatase