Vascular endothelial growth factor (VEGF) suppresses ovarian granulosa cell apoptosis in vitro.
Vascular endothelial growth factor (VEGF) inhibits the follicular atresia that resulted from granulosa cell apoptosis in the mammalian ovary. In the present study, we examined the effect of VEGF on granulosa cell apoptosis. Here, we report that VEGF suppresses granulosa cell apoptosis by inhibiting the release of caspase-activated DNase (CAD) without being associated with the mitochondrial pathway. VEGF did not stimulate or inhibit Bcl-xL and Bax, respectively, in granulosa cells. In addition, VEGF did not suppress the expression of active caspase-3, whereas follicle-stimulating hormone (FSH) inhibited caspase-3. However, VEGF and FSH suppressed the release of CAD resulting from the disintegration of the CAD-ICAD complex. These results demonstrate that VEGF is a strong survival factor for granulosa cell apoptosis (ovarian follicular atresia).
Kosaka
Naomichi
N
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Inada-machi, Obihiro, Hokkaido 080-8555, Japan.
Sudo
Natsuko
N
Miyamoto
Akio
A
Shimizu
Takashi
T
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D015153:Blotting, Western; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D003851:Deoxyribonucleases / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008954:Models, Biological; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000235:genetics; D051020:bcl-X Protein / Q000235:genetics
D051017:Apoptosis Regulatory Proteins; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; C109664:caspase-activated DNase inhibitor; D005640:Follicle Stimulating Hormone; D003851:Deoxyribonucleases; C109663:caspase-activated deoxyribonuclease; D053148:Caspase 3