title: Platelet function under high-shear conditions from platelet concentrates.
abstract: BACKGROUND
  Platelet (PLT) collection and storage affect the functional capacity of PLTs in PLT concentrates (PCs). Therefore, PLTs' functional quality should be studied before transfusion.
  
  
  METHODS
  PCs (n = 15) were collected by a standard apheresis procedure (Trima, Gambro BCT) and were stored for 7 days. Samples were taken to assess PLT adhesion and aggregate formation by a cone and plate analyzer (Impact-R, DiaMed) on Days 1, 3, 5, and 7 after harvesting. This device allows testing PLT function under high-shear stress close to physiologic conditions. Concomitantly, P-selectin expression and the residual responsiveness to TRAP-6 were determined by flow cytometry.
  
  
  RESULTS
  PLT adhesion, as measured by surface coverage, decreased during the entire observation period; likewise, the size of aggregates was significantly lower on Days 5 and 7 compared to Day 1 (p < 0.02). P-selectin expression increased from Day 5 to Day 7 (p < 0.04), whereas TRAP-6-inducible expression remained stable until Day 5 of storage and decreased significantly on Day 7 (p = 0.04).
  
  
  CONCLUSIONS
  Our results show that high-shear-induced PLT adhesion and aggregation on the polystyrene surface deteriorate upon storage, suggesting decreased PLT function in vivo. Thus, the Impact-R may be a useful tool to assess the functional capacity of PLTs under various PLT harvesting and storage procedures.
authors: Department of Blood Group Serology and Transfusion Medicine and Clinical Pharmacology, Medical University of Vienna, Vienna, Austria. petra.jilma@meduniwien.ac.at
authors: Petra
authors: P
authors: Jilma-Stohlawetz
authors: Michaela
authors: M
authors: Horvath
authors: Beate
authors: B
authors: Eichelberger
authors: Daniela
authors: D
authors: Koren
authors: Bernd
authors: B
authors: Jilma
authors: Simon
authors: S
authors: Panzer
mesh_terms: D000328:Adult; D001792:Blood Platelets; D001793:Blood Preservation; D006801:Humans; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000032:analysis; D010446:Peptide Fragments / Q000032:analysis; D015539:Platelet Activation; D010973:Platelet Adhesiveness; D010974:Platelet Aggregation; D010979:Platelet Function Tests / Q000295:instrumentation* / Q000592:standards; D013314:Stress, Mechanical; D013997:Time Factors
chemical_list: D019007:P-Selectin; D010446:Peptide Fragments; C082835:thrombin receptor peptide (42-47)