Influence of TRPV3 mutation on hair growth cycle in mice.
We recently reported that Gly573Ser substitution of the transient receptor potential cation channel, subfamily V member 3 (TRPV3) caused hair loss in DS-Nh mice. To further elucidate the effects of this mutation on the development of the spontaneous hairless phenotype, we examined the temperature-response to epidermal sheets from DS-Nh and DS mice. It was indicated that the mutation was gain-of-function. We also performed genetic and histological analyses with both strain skins. DNA microarray data revealed that the levels of keratin-associated protein 16-1, 16-3, and 16-9 genes related to the anagen phase were decreased in the skins of DS-Nh mice compared with those of three days old DS mice. Histological analysis revealed that the anagen phase persisted in DS-Nh mice, and that the telogen phase was seen in DS but not DS-Nh mice at 21 days of age. Regulation of TRPV3 appears to be important for appropriate hair development in rodents.
Imura
Kinichi
K
Department of Biosystems Science, Graduate School of Science and Technology, Kobe University, Kobe, Japan.
Yoshioka
Takeshi
T
Hikita
Ichiro
I
Tsukahara
Kiyoshi
K
Hirasawa
Tsutomu
T
Higashino
Kenichi
K
Gahara
Yoshinari
Y
Arimura
Akinori
A
Sakata
Tsuneaki
T
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism / Q000493:pharmacokinetics; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D006197:Hair / Q000254:growth & development* / Q000378:metabolism; D018859:Hair Follicle / Q000254:growth & development / Q000378:metabolism; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050916:TRPV Cation Channels / Q000235:genetics / Q000502:physiology*; D013997:Time Factors
D050916:TRPV Cation Channels; C486760:Trpv3 protein, mouse; D002118:Calcium