Platelet activation during haemodialysis: comparison of cuprammonium rayon and polysulfone membranes.
OBJECTIVE
Haemodialysis-treated patients are at a high risk of developing cardiovascular disease. Part of this risk may be attributable to the type of the dialysis membrane used. We evaluated whether different dialysis membranes differ with respect to platelet activation.


METHODS
In a randomized crossover trial, the platelet activation was measured in 14 patients treated with two different dialyzers (cuprammonium rayon membrane and polysulfone membrane). We compared the platelet activation over the dialyzer and between dialyzers after several weeks of dialysis.


RESULTS
There were no differences between the two dialyzers in platelet activation over the dialyzer. After 2 weeks, however, the expression of CD62P, CD63, and PAC-1 was statistically significantly lower after cuprammonium membrane treatment than after polysulfone membrane treatment (mean fluorescence intensity in arbitrary units 8.0 vs. 11.1, 2.64 vs. 4.01, and 5.61 vs. 9.74, respectively).


CONCLUSIONS
Dialysis with a polysulfone membrane seems to lead to more platelet activation than dialysis with a cuprammonium membrane.
Thijs
Abel
A
Department of Internal Medicine, VU University Medical Centre, Amsterdam, The Netherlands. a.thijs@vumc.nl
Grooteman
Muriel P C
MP
Zweegman
Sonja
S
Nubé
Menso J
MJ
Huijgens
Peter C
PC
Stehouwer
Coen D A
CD
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002482:Cellulose / Q000031:analogs & derivatives* / Q000592:standards; D054641:Dual Specificity Phosphatase 2 / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D019007:P-Selectin / Q000032:analysis; D015539:Platelet Activation; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D011108:Polymers / Q000592:standards*; D006435:Renal Dialysis / Q000009:adverse effects* / Q000295:instrumentation*; D013450:Sulfones / Q000592:standards*; D060149:Tetraspanin 30
D015703:Antigens, CD; D015415:Biomarkers; C554586:CD63 protein, human; D008567:Membranes, Artificial; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D011108:Polymers; D013450:Sulfones; D060149:Tetraspanin 30; C066013:AM 160H; C017662:polysulfone P 1700; D002482:Cellulose; C517032:DUSP2 protein, human; D054641:Dual Specificity Phosphatase 2