Subcellular distribution of trace elements in kidney of a mother-fetus pair of Dall's porpoises (Phocoenoides dalli).
Total and subcellular renal Zn, Cu, Se, Mn, V, Hg, Cd and Ag were determined by a mother-fetus pair of Dall's porpoises (Phocoenoides dalli). All element concentrations in the maternal individual were higher than those in the fetal individual. Most of total renal elements studied were present in the cytosol of both animals. In maternal cytosol, Mn, Hg and Ag were present in high molecular weight substances (HMW); Se was in low molecular weight substances (LMW); Zn, Cu, and Cd were in metallothionein (MT), mostly; and the distribution of V in percentage among the three renal cytosolic fractions was similar. In fetal cytosol, Zn, Mn, Hg, V, Cd, and Ag were present in HMW, Cu was present in MT, mostly. In contrast, Se was observed mostly in both LMW and HMW. MT isoforms were characterized. Three obvious peaks in retention time were found in either the maternal or fetal MT. The highest elemental ion intensities were in the 7.8min peak for the mother, and in the 4.3min peak for the fetus, respectively, implying that different MT isoforms may be closely associated with elemental accumulation between maternal and fetal renal cytosols.
Yang
Jian
J
Key Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Qitang Road No. 1, Wuxi City, Jiangsu 214081, China. jiany@ffrc.cn
Kunito
Takashi
T
Anan
Yasumi
Y
Tanabe
Shinsuke
S
Miyazaki
Nobuyuki
N
D000818:Animals; D002104:Cadmium / Q000493:pharmacokinetics; D002850:Chromatography, Gel; D003600:Cytosol / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D008345:Manganese / Q000493:pharmacokinetics; D008431:Maternal-Fetal Exchange; D008628:Mercury / Q000493:pharmacokinetics; D008668:Metallothionein / Q000493:pharmacokinetics; D019576:Porpoises; D011247:Pregnancy; D012834:Silver / Q000493:pharmacokinetics; D014018:Tissue Distribution; D014131:Trace Elements / Q000493:pharmacokinetics*; D014639:Vanadium / Q000493:pharmacokinetics; D015032:Zinc / Q000493:pharmacokinetics
D014131:Trace Elements; D002104:Cadmium; D014639:Vanadium; D012834:Silver; D008345:Manganese; D008668:Metallothionein; D008628:Mercury; D015032:Zinc