Development of a novel endothelial cell-seeded endovascular stent for intracranial aneurysm therapy.
The metallic stent has been widely used in endovascular treatment of intracranial aneurysms and arterial stenosis. Endothelialization at the neck of the aneurysm or stenotic lesion after stent deployment plays a pivotal role in preventing aneurysm recurrence, as well as local thrombus formation and restenosis. To deliver autologous endothelial cells and to promote the endothelialization on the luminal wall of the parent artery, we established an endothelial cell-seeded intracranial stent device. Endothelial cells were isolated from canine jugular vein and identified by FACS assay and immunohistochemistry. We demonstrated that the seeded endothelial cells formed a confluent endothelial layer on the stent's surface. After being brushed with 100 dyne/cm(2) of shear stress, we found that this endothelial layer remained intact for at least 48 h on the heparinized polymer coated stent, rather than the poly-lactic-acid coated stent (p < 0.05). The results suggest that an autologous endothelial cell-seeded stent may be a feasible and optimal tool for endothelial delivery during stenting and may overcome some limitations of the traditional bare stent in the treatment of intracranial aneurysms and arterial stenosis.
Zhu
Wei
W
Department of Neurosurgery, Huashan Hospital of Fudan University, Shanghai, China.
Tian
Yanlong
Y
Zhou
Liang-Fu
LF
Wang
Yang
Y
Song
Donglei
D
Mao
Ying
Y
Yang
Guo-Yuan
GY
D000818:Animals; D018929:Cell Culture Techniques; D020099:Coated Materials, Biocompatible / Q000592:standards*; D004285:Dogs; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology; D006493:Heparin; D020007:Implants, Experimental; D002532:Intracranial Aneurysm / Q000628:therapy*; D019344:Lactic Acid; D011091:Polyesters; D011108:Polymers; D015607:Stents; D013314:Stress, Mechanical
D020099:Coated Materials, Biocompatible; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D006493:Heparin