title: Immunolocalization of a lipase-like protein in the reproductive apparatus of female Phlebotomus papatasi, at various stages of the gonotrophic cycle.
abstract: In female phlebotomine sandflies, little is known about the reproductive accessory glands that presumably contribute to egg production and/or oviposition. The main protein secreted in the accessory glands of female Phlebotomus papatasi was recently characterised as a lipase-like protein, the first to be found in the female accessory glands of any insect. This protein, named PhpaLIP (for Phlebotomus papatasi lipase), has now been detected and localized in the reproductive tissues of female P. papatasi, at different stages of the gonotrophic cycle, using a polyclonal anti-PhpaLIP serum and both confocal scanning laser and immuno-electron microscopy. PhpaLIP appears to be always present in the accessory glands (with a secretory peak shortly before oviposition) but was also detected in the follicle cells of the ovarioles, within the developing vitelline envelope, and in the oviducts. The results are discussed in relation to the functions that PhpaLIP could have during the gonotrophic cycle, in the various reproductive structures of female P. papatasi.
authors: Dipartimento di Scienze Ambientali, Largo dell'Università, Università della Tuscia, 01100 Viterbo, Italy. ma_cri@inwind.it
authors: M
authors: M
authors: Belardinelli
authors: L
authors: L
authors: Guerra
authors: G
authors: G
authors: Gambellini
authors: M
authors: M
authors: Maroli
authors: M
authors: M
authors: Mazzini
authors: A M
authors: AM
authors: Fausto
mesh_terms: D000818:Animals; D005260:Female; D005836:Genitalia, Female / Q000201:enzymology; D019476:Insect Proteins / Q000032:analysis*; D008049:Lipase / Q000032:analysis*; D010058:Oviposition; D010691:Phlebotomus / Q000033:anatomy & histology; D012098:Reproduction / Q000502:physiology
chemical_list: D019476:Insect Proteins; D008049:Lipase