Cartilage oligometric matrix protein-angiopoietin-1 promotes revascularization through increased survivin expression in dermal endothelial cells of skin grafts in mice.
The present study examined the effects of cartilage oligometric matrix protein angiopoietin-1 (COMP-Ang1) on the revascularization of mice skin grafts. Full-thickness skin grafts were autotransferred into BALB/c mice. The donor grafts were soaked in COMP-Ang1 protein (50 mug/ml, n = 10) or in bovine serum albumin (BSA) (50 mug/ml, n = 10) dissolved in 1 ml of sterile, phosphate-buffered saline for 5 minutes before transfer. Revascularization of the grafts was monitored using an intravital microscope on postoperative days 3, 4, and 5. Morphological and immunohistochemical analyses were performed to evaluate platelet-endothelial cell adhesion molecule-1 and survivin expression and apoptotic signal in the transplanted grafts. Grafts soaked in COMP-Ang1 (COMP-Ang1 group) showed significantly increased revascularization compared with grafts soaked in BSA (BSA group) on intravital microscopy and platelet-endothelial cell adhesion molecule-1 staining. The COMP-Ang1 group showed a significant increase of survivin expression in the endothelial cells and a reduction of apoptotic signal in comparison to the BSA group. Therefore, we believe that COMP-Ang1 provides the therapeutic benefit of enhancing the survival of vascular endothelial cells during transplantation of skin graft.
Byun
Seung-Jae
SJ
Institute for Radiological Imaging Science, Wonkwang University School of Medicine, Jeonbuk, Korea.
Choi
Kyu-Sil
KS
Park
Sung Hoon
SH
Cho
Nam Woo
NW
Hyun Yoo
Chang
C
Yun
Ki Jung
KJ
Koh
Young Jun
YJ
Koh
Gou Young
GY
So
Byung Jun
BJ
Yoon
Kwon-Ha
KH
D000818:Animals; D017209:Apoptosis; D020405:Dermis / Q000098:blood supply*; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D051034:Inhibitor of Apoptosis Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D018384:Oxidative Stress; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000494:pharmacology*; D012097:Repressor Proteins; D016038:Skin Transplantation; D000077022:Survivin
C117749:Birc5 protein, mouse; C515576:COMP-Ang1 fusion protein; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D000077022:Survivin