Escherichia coli isolated from a Crohn's disease patient adheres, invades, and induces inflammatory responses in polarized intestinal epithelial cells.
Inflammatory diseases of the intestinal tract are a major health concern both in the United States and around the world. Evidence now suggests that a new category of Escherichia coli, designated Adherent Invasive E. coli (AIEC) is highly prevalent in Crohn's Disease (CD) patients. AIEC strains have been shown to colonize and adhere to intestinal epithelial cells (IEC). However, the role AIEC strains play in the induction of an inflammatory response is not known. Therefore, we examined several E. coli strains (designated LF82, O83:H1, 6604 and 6655) that were isolated from CD patients for their ability to induce inflammation in two IEC, Caco-2BBe and T-84 cells. Results showed that each strain had varying abilities to adhere to and invade IEC as well as induced cytokine secretion from polarized IEC. However, E. coli O83:H1 displayed the best characteristics of AIEC strains as compared to the prototype AIEC strain LF82, inducing cytokine secretion from IEC and promoting immune cell migration through IEC. Upon further analysis, E. coli O83:H1 did not harbor virulence genes present in known pathogenic intestinal organisms. Further characterization of E. coli O83:H1 virulence determinants showed that a non-flagellated O83:H1 strain significantly decreased the organism's ability to adhere to and invade both IEC and elicit IEC cytokine secretion compared to the wild type and complemented strains. These findings demonstrate that E. coli O83:H1 possesses the characteristics of the AIEC LF82 strain that may contribute to the low-grade, chronic inflammation observed in Crohn's disease.
Eaves-Pyles
Tonyia
T
Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Blvd., Galveston, Texas 77555-1070, USA.
Allen
Christopher A
CA
Taormina
Joanna
J
Swidsinski
Alexander
A
Tutt
Christopher B
CB
Jezek
G Eric
GE
Islas-Islas
Martha
M
Torres
Alfredo G
AG
D001422:Bacterial Adhesion; D002460:Cell Line; D054443:Cell Migration Assays; D002465:Cell Movement; D003424:Crohn Disease / Q000382:microbiology*; D016207:Cytokines / Q000096:biosynthesis; D004269:DNA, Bacterial / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology; D004847:Epithelial Cells / Q000382:microbiology*; D004926:Escherichia coli / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D005407:Flagella / Q000235:genetics / Q000502:physiology; D017353:Gene Deletion; D005816:Genetic Complementation Test; D006801:Humans; D009504:Neutrophils / Q000276:immunology; D014481:United States; D037521:Virulence Factors / Q000235:genetics
D016207:Cytokines; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D037521:Virulence Factors