Peptidic soil components are a major dietary resource for the humivorous larvae of Pachnoda spp. (Coleoptera: Scarabaeidae).
Humivorous scarab beetle larvae can thrive exclusively on soil organic matter. Feeding experiments have revealed that the larva of Pachnoda ephippiata mineralizes all major humus components except the polyphenolic fraction. High proteolytic activity in the alkaline midgut fluid and an enormous ammonia production during gut passage suggested that peptidic soil components are an important dietary resource for the larva. By comparing acid-hydrolyzable amino acids in food soil and feces, we showed that a significant fraction of the peptides in soil are removed during gut passage. This agrees well with the high concentrations of free amino acids found the midgut section. Incubation experiments revealed the presence of substantial particle-associated proteolytic activity also in the hindgut, most probably due to microbial activity. High rates of ammonia formation in hindgut homogenates and the conversion of radiolabeled amino acids to acetate and propionate indicated that microbial fermentations of soil peptides play an important role in the hindgut. This was corroborated by viable counts of amino-acid-fermenting bacteria, which formed a substantial fraction of the hindgut microbiota. A complete inventory of organic and inorganic nitrogen species before, during, and after gut passage revealed the formation of nitrite and nitrate in midgut and hindgut, and a substantial nitrogen deficit in the feces, suggesting that part of the ammonia formed by mineralization is subjected to oxidation and subsequent denitrification to N2. Together, the results strongly support the hypothesis that peptidic soil components form a major energy and nutrient source for humivorous insects, supplying the animal with microbial fermentation products and essential amino acids.
Andert
Janet
J
Department of Biogeochemistry, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany.
Geissinger
Oliver
O
Brune
Andreas
A
D000596:Amino Acids / Q000032:analysis; D000641:Ammonia / Q000378:metabolism; D000824:Animal Nutritional Physiological Phenomena / Q000502:physiology*; D000818:Animals; D002250:Carbon Radioisotopes / Q000378:metabolism; D001517:Coleoptera / Q000382:microbiology / Q000502:physiology*; D004064:Digestive System / Q000382:microbiology*; D005243:Feces / Q000737:chemistry; D005247:Feeding Behavior / Q000502:physiology*; D007814:Larva / Q000382:microbiology / Q000502:physiology; D010455:Peptides / Q000737:chemistry; D012987:Soil / Q000032:analysis*; D013997:Time Factors
D000596:Amino Acids; D002250:Carbon Radioisotopes; D010455:Peptides; D012987:Soil; D000641:Ammonia