[Ultrastructural evaluation of mitomycin C toxic effects on the ciliary epithelium of normal rabbits eyes with and without aqueous humor suppressant treatment].
OBJECTIVE
To evaluate the effects of mitomycin C (MMC) on the internal ciliary epithelium (ICE) of the ciliary body of animals treated with two different aqueous humor suppressants.


METHODS
The eyes of sixteen Norfolk albino rabbits divided into four experimental groups were studied. The right eyes (RE) of the four groups received 0.1 ml of MMC (0.5 mg/ml) under the scleral flap. The left eyes (LE) was the control group. Group 1 (G1) did not have any other treatment. To Group 2 (G2) and Group 4 (G4) acetazolamide was administered. To Group (G3) and Group 4 (G4) timolol maleate was administered. ICE was examined by transmission electron microscopy (TEM).


RESULTS
The following aspects were observed in all groups, except in G1 LE: cell shrinkage and/or enlargement of intercellular spaces, rarefied mitochondria, clear vesicular structures and electron-dense bodies. The internal limitant membrane showed to be thickened, discontinued and separated in all groups, except in G1 LE and G2 LE. Discharge of cytoplasmatic material was observed only in the groups treated with aqueous humor suppressants.


CONCLUSIONS
1) MMC, acetazolamide and timolol maleate caused morphological alterations in the ciliary epithelium even when used alone. 2) The combination of MMC and acetazolamide caused more alterations than did isolated acetazolamide, but not more than MMC alone. 3) For the other combinations the alterations were similar.
Hamada
André
A
Programa de Pós-Graduação Bases Gerais da Cirurgia, Faculdade de Medicina, Universidade Estadual Paulista, Botucatu, SP, Brasil.
Silva
Maria Rosa Bet de Moraes
MR
Gregório
Elisa Aparecida
EA
D000086:Acetazolamide / Q000009:adverse effects / Q000627:therapeutic use; D000319:Adrenergic beta-Antagonists / Q000009:adverse effects / Q000627:therapeutic use; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D001082:Aqueous Humor / Q000187:drug effects*; D002257:Carbonic Anhydrase Inhibitors / Q000009:adverse effects / Q000627:therapeutic use; D002924:Ciliary Body / Q000187:drug effects / Q000648:ultrastructure; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D008854:Microscopy, Electron; D016685:Mitomycin / Q000008:administration & dosage / Q000633:toxicity*; D023421:Models, Animal; D011817:Rabbits; D011897:Random Allocation; D012590:Sclera / Q000601:surgery*; D013524:Surgical Flaps; D013999:Timolol / Q000009:adverse effects / Q000627:therapeutic use
D000319:Adrenergic beta-Antagonists; D000903:Antibiotics, Antineoplastic; D002257:Carbonic Anhydrase Inhibitors; D016685:Mitomycin; D013999:Timolol; D000086:Acetazolamide