Rapid determination of antibiotic resistance in E. coli using dielectrophoresis.
In recent years, infections due to antibiotic-resistant strains of bacteria such as methicillin-resistant Staphylococcus aureus and ciprofloxacin-resistant Escherichia coli are on the rise, and with them the demand for rapid antibiotic testing is also rising. Conventional tests, such as disc diffusion testing, require a primary sample to be tested in the presence of a number of antibiotics to verify which antibiotics suppress growth, which take approximately 24 h to complete and potentially place the patient at severe risk. In this paper we describe the use of dielectrophoresis as a rapid marker of cell death, by detecting changes in the electrophysiology of the cell caused by the administration of an antibiotic. In contrast to other markers, the electrophysiology of the cell changes rapidly during cell death allowing live cells to be distinguished from dead (or dying) cells without the need for culturing. Using polymyxin B as an example antibiotic, our studies indicate that significant changes in cell characteristics can be observed as soon as 1 h passes after isolating a culture from nutrient broth.
Hoettges
Kai F
KF
School of Engineering, University of Surrey, Guildford, Surrey GU2 7XH, UK.
Dale
Jeremy W
JW
Hughes
Michael P
MP
D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D017209:Apoptosis / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects*; D024881:Drug Resistance, Bacterial / Q000502:physiology*; D017097:Electric Impedance; D004586:Electrophoresis / Q000379:methods*; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents