title: Effects of scutellarin on PKCgamma in PC12 cell injury induced by oxygen and glucose deprivation.
abstract: OBJECTIVE
  To evaluate the neuroprotective effect and mechanisms of scutellarin (Scu) against PC12 cell injury after oxygen and glucose deprivation followed by reperfusion (OGD-Rep).
  
  
  METHODS
  Undifferentiated rat pheochromocytoma PC12 cells, exposed to oxygen and glucose deprivation followed by reperfusion (OGD-Rep), used as an in vitro model of ischemia/reperfusion. Cell survival was evaluated by diphenyltetrazolium bromide (MTT) assay and the amount of LDH release was determined using assay kits. [Ca2+](i) was monitored using a fluorescent Ca2+-sensitive dye Fura-2 acetoxymethyl ester. Cell apoptosis was detected by a DNA ladder and by flow cytometric detection. The expression of protein kinase C (PKC)gamma was determined using both RT-PCR and Western blotting. The translocation of PKCgamma was assayed by subcellular fractionation and Western blotting.
  
  
  RESULTS
  OGD-Rep injury significantly elevated the level of LDH release, [Ca2+](i), mRNA expression and the translocation of PKCgamma compared in the PC12 cells with those of the normal group. Scu (10-100 micromol/L) exerted a protective effect against OGD-Rep injury by reducing LDH release, [Ca2+](i), the percent of apoptosis, and the translocation of PKCgamma.
  
  
  CONCLUSIONS
  Scu inhibits the increase of [Ca2+](i) and the activation of PKCgamma, exerting protective effects against PC12 cell injury induced by OGD-Rep.
authors: State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China.
authors: Wei
authors: W
authors: Xu
authors: Ruo-Peng
authors: RP
authors: Zha
authors: Wen-Yi
authors: WY
authors: Wang
authors: Yi-Ping
authors: YP
authors: Wang
mesh_terms: D000818:Animals; D047310:Apigenin / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001692:Biological Transport / Q000187:drug effects; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D015687:Cell Hypoxia; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D049919:Erigeron / Q000737:chemistry; D005434:Flow Cytometry; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D005965:Glucuronates / Q000302:isolation & purification / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism / Q000494:pharmacology; D016716:PC12 Cells; D010946:Plants, Medicinal / Q000737:chemistry; D011493:Protein Kinase C / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D005965:Glucuronates; D018696:Neuroprotective Agents; D012333:RNA, Messenger; C484876:scutellarin; D047310:Apigenin; D007770:L-Lactate Dehydrogenase; C087857:protein kinase C gamma; D011493:Protein Kinase C; D005947:Glucose; D010100:Oxygen; D002118:Calcium