title: Effect of indenter size on elastic modulus of cartilage measured by indentation.
abstract: Our preliminary indentation experiments showed that the equilibrium elastic modulus of murine tibial cartilage increased with decreasing indenter size: flat-ended 60 deg conical tips with end diameters of 15 microm and 90 microm gave 1.50+/-0.82 MPa (mean+/-standard deviation) and 0.55+/-0.11 MPa, respectively (p<0.01). The goal of this paper is to determine if the dependence on tip size is an inherent feature of the equilibrium elastic modulus of cartilage as measured by indentation. Since modulus values from nonindentation tests are not available for comparison for murine cartilage, bovine cartilage was used. Flat-ended conical or cylindrical tips with end diameters ranging from 5 microm to 4 mm were used to measure the equilibrium elastic modulus of bovine patellar cartilage. The same tips were used to test urethane rubber for comparison. The equilibrium modulus of the bovine patellar cartilage increased monotonically with decreasing tip size. The modulus obtained from the 2 mm and 4 mm tips (0.63+/-0.21 MPa) agreed with values reported in the literature; however, the modulus measured by the 90 microm tip was over two and a half times larger than the value obtained from the 1000 microm tip. In contrast, the elastic modulus of urethane rubber obtained using the same 5 microm-4 mm tips was independent of tip size. The equilibrium elastic modulus of bovine patellar cartilage measured by indentation depends on tip size. This appears to be an inherent feature of indentation of cartilage, perhaps due to its inhomogeneous structure.
authors: Department of Orthopaedic Surgery, University of Minnesota, Minneapolis, Minnesota 55455, USA.
authors: Narendra K
authors: NK
authors: Simha
authors: Hui
authors: H
authors: Jin
authors: Mellanie L
authors: ML
authors: Hall
authors: Sidharth
authors: S
authors: Chiravarambath
authors: Jack L
authors: JL
authors: Lewis
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000379:methods; D002358:Cartilage, Articular / Q000502:physiology* / Q000648:ultrastructure; D002417:Cattle; D019245:Compressive Strength; D004548:Elasticity; D010329:Patella; D015203:Reproducibility of Results; D013314:Stress, Mechanical; D014520:Urethane / Q000737:chemistry
chemical_list: D014520:Urethane