title: c-Myb is required for neural progenitor cell proliferation and maintenance of the neural stem cell niche in adult brain.
abstract: Ongoing production of neurons in adult brain is restricted to specialized neurogenic niches. Deregulated expression of genes controlling homeostasis of neural progenitor cell division and/or their microenvironment underpins a spectrum of brain pathologies. Using conditional gene deletion, we show that the proto-oncogene c-myb regulates neural progenitor cell proliferation and maintains ependymal cell integrity in mice. These two cellular compartments constitute the neurogenic niche in the adult brain. Brains devoid of c-Myb showed enlarged ventricular spaces, ependymal cell abnormalities, and reduced neurogenesis. Neural progenitor cells lacking c-Myb showed a reduced intrinsic proliferative capacity and reduction of Sox-2 and Pax-6 expression. These data point to an important role for c-Myb in the neurogenic niche of the adult brain.
authors: Differentiation and Transcription Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia.
authors: Jordane
authors: J
authors: Malaterre
authors: Theo
authors: T
authors: Mantamadiotis
authors: Sebastian
authors: S
authors: Dworkin
authors: Sally
authors: S
authors: Lightowler
authors: Qing
authors: Q
authors: Yang
authors: Mark I
authors: MI
authors: Ransome
authors: Ann M
authors: AM
authors: Turnley
authors: Nancy R
authors: NR
authors: Nichols
authors: Nikla R
authors: NR
authors: Emambokus
authors: Jon
authors: J
authors: Frampton
authors: Robert G
authors: RG
authors: Ramsay
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D002452:Cell Count; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D005136:Eye Proteins / Q000096:biosynthesis; D015870:Gene Expression; D005786:Gene Expression Regulation; D020570:Genes, myb; D018398:Homeodomain Proteins / Q000096:biosynthesis; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020287:In Situ Nick-End Labeling; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D012097:Repressor Proteins / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055748:SOXB1 Transcription Factors; D015534:Trans-Activators / Q000096:biosynthesis
chemical_list: D004268:DNA-Binding Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; C000605732:Pax6 protein, mouse; D012333:RNA, Messenger; D012097:Repressor Proteins; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; D015534:Trans-Activators