Tumor immunolocalization using 124 I-iodine-labeled JAA-F11 antibody to Thomsen-Friedenreich alpha-linked antigen.
Clinical immunolocalization has been attempted by others with an anti-Thomsen-Friedenreich antigen (TF-Ag) mAb that bound both alpha- and beta-linked TF-Ag. In this report, 124 I-labeled mAb JAA-F11 specific for alpha-linked TF-Ag showed higher tumor specificity in in vivo micro-positron emission tomography (micro-PET) of the mouse mammary adenocarcinoma line, 4T1, showing no preferential uptake by the kidney. Labeled product remained localized in the tumor for at least 20 days. Glycan array analysis showed structural specificity of the antibody.
Chaturvedi
Richa
R
Department of Biotechnical and Clinical Laboratory Sciences, University at Buffalo, The State University of New York, Buffalo, NY 14214, USA.
Heimburg
Jamie
J
Yan
Jun
J
Koury
Stephen
S
Sajjad
Munawwar
M
Abdel-Nabi
Hani H
HH
Rittenhouse-Olson
Kate
K
D000230:Adenocarcinoma / Q000000981:diagnostic imaging* / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000493:pharmacokinetics; D000918:Antibody Specificity; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology*; D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D005260:Female; D018796:Immunoconjugates / Q000276:immunology / Q000493:pharmacokinetics; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics; D008325:Mammary Neoplasms, Experimental / Q000000981:diagnostic imaging* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000276:immunology / Q000493:pharmacokinetics; D049268:Positron-Emission Tomography
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate; D018796:Immunoconjugates; D007457:Iodine Radioisotopes; D011134:Polysaccharides; C016467:Thomsen-Friedenreich antigen