title: Connector inversion probe technology: a powerful one-primer multiplex DNA amplification system for numerous scientific applications.
abstract: We combined components of a previous assay referred to as Molecular Inversion Probe (MIP) with a complete gap filling strategy, creating a versatile powerful one-primer multiplex amplification system. As a proof-of-concept, this novel method, which employs a Connector Inversion Probe (CIPer), was tested as a genetic tool for pathogen diagnosis, typing, and antibiotic resistance screening with two distinct systems: i) a conserved sequence primer system for genotyping Human Papillomavirus (HPV), a cancer-associated viral agent and ii) screening for antibiotic resistance mutations in the bacterial pathogen Neisseria gonorrhoeae. We also discuss future applications and advances of the CIPer technology such as integration with digital amplification and next-generation sequencing methods. Furthermore, we introduce the concept of two-dimension informational barcodes, i.e. "multiplex multiplexing padlocks" (MMPs). For the readers' convenience, we also provide an on-line tutorial with user-interface software application CIP creator 1.0.1, for custom probe generation from virtually any new or established primer-pairs.
authors: Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America.
authors: Michael S
authors: MS
authors: Akhras
authors: Magnus
authors: M
authors: Unemo
authors: Sreedevi
authors: S
authors: Thiyagarajan
authors: Pål
authors: P
authors: Nyrén
authors: Ronald W
authors: RW
authors: Davis
authors: Andrew Z
authors: AZ
authors: Fire
authors: Nader
authors: N
authors: Pourmand
mesh_terms: D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D004352:Drug Resistance, Microbial / Q000235:genetics; D009154:Mutation
chemical_list: D015342:DNA Probes; D004247:DNA