A novel flavonoid from Lespedeza virgata (Thunb.) DC.: structural elucidation and antioxidative activity.
Bioactivity guided fractionation of the ethanolic extract of the whole plants of Lespedeza virgata (Thunb.) DC. resulted in the isolation of a novel flavonoid (1) along with five known compounds 2-6. The molecular and structural formula as well as the stereochemistry of compound 1 were determined using data obtained from (1)H and (13)C NMR spectra, DEPT135 and by 2D HSQC, HMBC, (1)H-(1)H correlated spectroscopy ((1)H-(1)H COSY), and nuclear overhauser effect spectroscopy (NOESY) experiments. The superoxide anion scavenging activities of all isolated compounds were evaluated by the hypoxanthine nitro blue tetrazolium and ESR methods and the new compound 1 showed the strongest antioxidative activity 95.79% (IC(50)=0.14 mg/ml).
Tan
Li
L
State Key Laboratory for SCUSS, Institute of Chemistry, The Chinese Academy of Sciences, Beijing, PR China.
Zhang
Xiu-Feng
XF
Yan
Bao-Zhen
BZ
Shi
Hai-Ming
HM
Du
Li-Bo
LB
Zhang
Ya-Zhou
YZ
Wang
Lan-Fen
LF
Tang
Ya-Lin
YL
Liu
Yang
Y
D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D004578:Electron Spin Resonance Spectroscopy; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D020128:Inhibitory Concentration 50; D029884:Lespedeza / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D012997:Solvents / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry
D000975:Antioxidants; D005419:Flavonoids; D012997:Solvents; D013481:Superoxides; D000431:Ethanol