title: Molecular profiling of proteolytic and lectin transcripts in Homalodisca vitripennis (Hemiptera: Auchenorrhyncha: Cicadellidae) feeding on sunflower and cowpea.
abstract: Homalodisca vitripennis Germar 1821 (Hemiptera: Cicadellidae) [Takiya et al. (2006) Ann Entomol Soc Am 99:648-655; syn. H. coagulata (Say)] salivary gland and gut EST libraries were used to isolate cDNA fragments of the gene transcripts encoding for cathepsin L, asparaginyl endopeptidase, cathepsin B, metalloendopeptidase, cathepsin D, multicatalytic endopeptidase, and a sugar-binding C-type lectin. Transcript levels were evaluated in immature and adult H. vitripennis feeding on sunflower (Helianthus annuus) or cowpea (Vigna unguiculata). Northern blot hybridization results showed that expression of most of the transcripts were similar for all developmental stages and feeding on the two diets examined. However, the expression of the transcript for asparaginyl endopeptidase was less expressed in sunflower-fed adult females compared to sunflower-fed immatures. Also, the expression of the C-type lectin transcript was up-regulated in adults compared to immatures when fed on either diet. Documenting both the presence and variation of transcript expression involved in putative digestive proteolysis in this xylem-feeding leafhopper is noteworthy and aids efforts to design specific diet formulations for mass production of hosts and parasitoids to be used as effective biological control measures.
authors: Biological Control of Insects Research Laboratory, USDA--Agriculture Research Service, Columbia, Missouri 65203, USA. coudront@missouri.edu
authors: Thomas A
authors: TA
authors: Coudron
authors: Sandra L
authors: SL
authors: Brandt
authors: Wayne B
authors: WB
authors: Hunter
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D020224:Expressed Sequence Tags; D007887:Fabaceae / Q000378:metabolism / Q000469:parasitology; D020869:Gene Expression Profiling; D006368:Helianthus / Q000378:metabolism / Q000469:parasitology; D006430:Hemiptera / Q000201:enzymology*; D037102:Lectins / Q000378:metabolism*; D009758:Nymph / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D014158:Transcription, Genetic; D052584:Xylem / Q000378:metabolism*
chemical_list: D037102:Lectins; D010940:Plant Proteins; D010447:Peptide Hydrolases