title: Global gene expression analysis of bovine blastocysts produced by multiple methods.
abstract: Reproductive efficiency using somatic cell nuclear transfer (SCNT) technology remains suboptimal. Of the various efforts to improve the efficiency, chromatin transfer (CT) and clone-clone aggregation (NTagg) have been reported to produce live cloned animals. To better understand the molecular mechanisms of somatic cell reprogramming during SCNT and assess the various SCNT methods on the molecular level, we performed gene expression analysis on bovine blastocysts produced via standard nuclear transfer (NT), CT, NTagg, in vitro fertilization (IVF), and artificial insemination (AI), as well as on somatic donor cells, using bovine genome arrays. The expression profiles of SCNT (NT, CT, NTagg) embryos were compared with IVF and AI embryos as well as donor cells. NT and CT embryos have indistinguishable gene expression patterns. In comparison to IVF or AI embryos, the number of differentially expressed genes in NTagg embryos is significantly higher than in NT and CT embryos. Genes that were differentially expressed between all the SCNT embryos and IVF or AI embryos are identified. Compared to AI embryos, more than half of the genes found deregulated between SCNT and AI embryos appear to be the result of in vitro culture alone. The results indicate that although SCNT methods have altered differentiated somatic nuclei gene expression to more closely resemble that of embryonic nuclei, combination of insufficient reprogramming and in vitro culture condition compromise the developmental potential of SCNT embryos. This is the first set of comprehensive data for analyzing the molecular impact of various nuclear transfer methods on bovine pre-implantation embryos.
authors: Viagen, Inc., Austin, Texas 78732, USA. wenli.zhou@viagen.com
authors: Wenli
authors: W
authors: Zhou
authors: Tianhao
authors: T
authors: Xiang
authors: Shawn
authors: S
authors: Walker
authors: Vicki
authors: V
authors: Farrar
authors: Earl
authors: E
authors: Hwang
authors: Brian
authors: B
authors: Findeisen
authors: Sanaz
authors: S
authors: Sadeghieh
authors: Fernando
authors: F
authors: Arenivas
authors: Ronald V
authors: RV
authors: Abruzzese
authors: Irina
authors: I
authors: Polejaeva
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D002417:Cattle; D019976:Cloning, Organism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D053652:Nuclear Transfer Techniques