Analysis of glycyrrhizic acid and liquiritin in liquorice root with microwave-assisted micellar extraction and pre-concentration.
The feasibility of employing a non-ionic surfactant (Triton X-100) as an alternative and effective solvent for the microwave-assisted extraction of glycyrrhizic acid and liquiritin from liquorice root has been demonstrated. When compared with commonly used solvents, 5% Triton X-100 yielded higher extraction efficiency than aqueous solutions of ethanol or methanol. Under optimal conditions, i.e. 5% Triton X-100 (v/v) and microwave-assisted extraction for 3-5 min at 100 degrees C, the percentage extraction of active ingredients reached the highest value. The pre-concentration factor for the glycyrrhizic acid and liquiritin was about 13, and the cloud-point extraction recoveries for the two ingredients were 98.4 and 96.1%, respectively. The results showed that the coupling of microwave-assisted extraction and cloud-point extraction could be employed as a new and effective approach for the rapid extraction and pre-concentration of pharmacologically active ingredients from liquorice root without disturbing the subsequent chromatographic analysis.
Sun
Chen
C
Chemistry and Chemical Engineering Department, Graduate University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
Xie
Yuchun
Y
Tian
Qinglai
Q
Liu
Huizhou
H
D044950:Flavanones / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D005960:Glucosides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D006035:Glycyrrhiza / Q000737:chemistry*; D019695:Glycyrrhizic Acid / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D008872:Microwaves; D015394:Molecular Structure; D017830:Octoxynol / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D012997:Solvents
D044950:Flavanones; D005960:Glucosides; D010936:Plant Extracts; D012997:Solvents; D019695:Glycyrrhizic Acid; D017830:Octoxynol; C512196:liquiritin