title: Preliminary X-ray crystallographic studies of a Lys49-phospholipase A2 homologue from Bothrops pirajai venom complexed with p-bromo-phenacyl bromide and alpha-tocopherol inhibitors.
abstract: PrTX-I, a non-catalytic and myotoxic Lys49-PLA(2) from Bothrops pirajai venom has been crystallized alone and in complex with bromophenacyl bromide (BPB), alpha tocopherol and alpha tocopherol acetate inhibitors. These crystals have shown to diffract X-rays between 2.34 and 1.65 A resolution. All complexes crystals are isomorphous and belong to the space group P2(1) whereas native PrTX-I crystals belong to the P3(1)21.
authors: Departamento de Física e Biofísica, Instituto de Biociências, UNESP, C. P. 510, CEP 18618-000, Botucatu-SP, Brazil.
authors: Juliana I
authors: JI
authors: Dos Santos
authors: Daniela P
authors: DP
authors: Marchi-Salvador
authors: Carlos A H
authors: CA
authors: Fernandes
authors: Lucas B
authors: LB
authors: Silveira
authors: Andreimar M
authors: AM
authors: Soares
authors: Marcos R M
authors: MR
authors: Fontes
mesh_terms: D000818:Animals; D017837:Bothrops; D001965:Bromides / Q000494:pharmacology*; D003435:Crotalid Venoms / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008239:Lysine / Q000737:chemistry*; D008958:Models, Molecular; D010741:Phospholipases A / Q000737:chemistry*; D011487:Protein Conformation; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D001965:Bromides; D003435:Crotalid Venoms; D004791:Enzyme Inhibitors; D010741:Phospholipases A; D024502:alpha-Tocopherol; D008239:Lysine