Effect of compressive force on the production of prostaglandin E(2) and its receptors in osteoblastic Saos-2 cells.
In orthodontic tooth movement, prostaglandin E(2) (PGE(2)) released from osteoblasts can alter the normal process of bone remodeling. We examined the effect of compressive force (CF) on PGE(2) production, PGE receptors (Ep1-4) expression, phosphorylation of protein kinase A (p-PKA), and calcium content in Saos-2 cells. PGE(2) production increased as CF strength. Applying CF of 98 or 294 Pa caused the cells to produce approximately 700 and 1,400 pg/mL PGE(2), respectively. CF of 98 Pa increased Ep2 gene expression, and 98 and 294 Pa CF increased Ep4. Immunohistochemical staining showed strong expression of Ep2 under 98 Pa and Ep4 under 98 and 294 Pa. The p-PKA increased as the strength of CF or PGE(2) concentration. The calcium content was increased by the addition of 700 pg/mL PGE(2) but was decreased by 1400 pg/mL. Thus, mechanical stress controls bone formation by stimulating PGE(2) production and Ep2 and/or Ep4 expression in osteoblasts.
Sanuki
Rina
R
Department of Orthodontics, Nihon University School of Dentistry, Tokyo, Japan.
Mitsui
Narihiro
N
Suzuki
Naoto
N
Koyama
Yuki
Y
Yamaguchi
Akikuni
A
Isokawa
Keitaro
K
Shimizu
Noriyoshi
N
Maeno
Masao
M
D002118:Calcium / Q000032:analysis; D045744:Cell Line, Tumor; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D015232:Dinoprostone / Q000096:biosynthesis*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D010766:Phosphorylation; D018078:Receptors, Prostaglandin E / Q000096:biosynthesis* / Q000302:isolation & purification; D013314:Stress, Mechanical
D018078:Receptors, Prostaglandin E; D017868:Cyclic AMP-Dependent Protein Kinases; D015232:Dinoprostone; D002118:Calcium