Design of a pyrene-containing fluorescence probe for labeling of RNA poly(A) tracts.
A labeling probe containing a pyrenecarboxamide-tethered modified DNA base, (Py)U, has been developed for fluorometric detection of RNA poly(A) tracts, in which the fluorescence emission intensity was controlled by the microstructural change around (Py)U caused by binding with the target RNA.
Tanaka
Kazuo
K
Frontier Research System, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama 351-0198, Japan.
Okamoto
Akimitsu
A
D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis*; D011721:Pyrenes; D012313:RNA / Q000032:analysis*; D014498:Uracil
D015342:DNA Probes; D005456:Fluorescent Dyes; D011721:Pyrenes; D011061:Poly A; D014498:Uracil; D012313:RNA; C030984:pyrene