[Recombinant adenovirus-mediated RNA silencing of tissue factor expression in human islet: an in vitro study].
OBJECTIVE
To construct a replication-incompetent recombinant adenovirus mediating short hairpin RNA (shRNA)-induced tissue factor gene silencing in the islet.


METHODS
Four pairs of complementary oligonucleotides were designed and synthesized to create double-stranded oligonucleotides (ds oligo). The ds oligos were cloned into Pentr/U6 vector to construct the shuttle plasmid pENTR/U6-shRNA, which was transduced into human islets via liposome after sequence verification. The plasmid with the best silencing effect was identified by real-time RT-PCR, followed by homologous recombination with the adenovirus backbone plasmid. The functional clone was transfected into 293A cells to amplify the adenovirus, whose silencing effect against TF expression was tested using real-time RT-PCR and Western blotting.


RESULTS
The pENTR/U6-shRNA shuttle plasmid was constructed and verified by sequencing. The recombinant adenovirus-mediated shRNA against TF was constructed, and real-time RT-PCR and Western blotting demonstrated that the strongest silencing effect of the adenovirus against TF occurred on the 4th day following islet transfection.


CONCLUSIONS
Replication-incompetent recombinant adenovirus-mediated shRNA against TF has been successfully constructed, which has good silencing effect against TF expression in human islet in vitro.
Li
Zhao-lun
ZL
Department of Kidney Transplantation, First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
Xu
Wu-jun
WJ
Tian
Pu-xun
PX
Ding
Xiao-ming
XM
Tian
Xiao-hui
XH
Feng
Xin-shun
XS
Hou
Jun
J
D000256:Adenoviridae / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D002460:Cell Line; D004274:DNA, Recombinant / Q000235:genetics*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D055029:Inverted Repeat Sequences; D007515:Islets of Langerhans / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013925:Thromboplastin / Q000172:deficiency* / Q000235:genetics*; D019562:Viral Load; D014779:Virus Replication
D004274:DNA, Recombinant; D034741:RNA, Small Interfering; D013925:Thromboplastin