Extrapancreatic organ impairment during acute pancreatitis induced by bile-pancreatic duct obstruction. Effect of N-acetylcysteine.
Multiple organ failure is frequently associated with acute pancreatitis (AP). Our aim was to study pulmonary, hepatic and renal complications developed in the course of AP experimentally induced in rats by bile-pancreatic duct obstruction (BPDO), differentiating the complications caused by AP itself, from those directly caused by bile duct obstruction (BDO), after ligating the choledocus. N-acetylcysteine (NAC) was administered as a therapeutic approach. Myeloperoxidase activity revealed neutrophil infiltration in lungs from 12 h after BDO, even if AP was not triggered. Lactate dehydrogenase (LDH) activity indicated hepatocyte death from 48 h after BDO, and from 24 h following BPDO-induced AP onwards, an effect delayed until 48 h by NAC treatment. Rats with single cholestasis (BDO) and rats with BPDO-induced AP showed a significant increase in plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT) and bilirubin concentration from 12 h onwards, whose values were reduced by NAC treatment at early BPDO. No renal failure was found during 120 h of bile-pancreatic obstruction. Our results showed lung and liver impairment as a result of BDO, even if AP does not develop. Pancreatic damage and extrapancreatic complications during AP induced by BPDO were palliated by NAC treatment.
Manso
Manuel A
MA
Department of Physiology and Pharmacology, University of Salamanca, Salamanca, Spain.
Ramudo
Laura
L
De Dios
Isabel
I
D000111:Acetylcysteine / Q000627:therapeutic use*; D000208:Acute Disease; D000410:Alanine Transaminase / Q000097:blood; D000681:Amylases / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002779:Cholestasis; D003404:Creatinine / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008026:Ligation; D008099:Liver / Q000473:pathology; D008168:Lung / Q000473:pathology; D008297:Male; D023421:Models, Animal; D009102:Multiple Organ Failure / Q000097:blood / Q000188:drug therapy / Q000473:pathology*; D010179:Pancreas / Q000473:pathology; D010195:Pancreatitis / Q000097:blood / Q000188:drug therapy / Q000473:pathology*; D009195:Peroxidase / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D016896:Treatment Outcome
D015415:Biomarkers; D003404:Creatinine; D007770:L-Lactate Dehydrogenase; D009195:Peroxidase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000681:Amylases; D001663:Bilirubin; D000111:Acetylcysteine