title: Overexpression of a metacaspase gene stimulates cell growth and stress response in Schizosaccharomyces pombe.
abstract: A unique gene named pca1(+), encoding a metacaspase, was cloned from the fission yeast Schizosaccharomyces pombe and was used to create a recombinant plasmid, pPMC. The metacaspase mRNA level was markedly elevated in the fission yeast cells harboring the plasmid pPMC. Overexpressed Pca1(+) appeared to stimulate the growth of the fission yeast cells instead of arresting their growth. Its expression was enhanced by stress-inducing agents such as H(2)O(2), sodium nitroprusside, and CdCl(2), and it conferred cytoprotection, especially against CdCl(2). However, such protection was not reproducible in the budding yeast Saccharomyces cerevisiae harboring pPMC. Taken together, these results propose that Pca1(+) may be involved in the growth and stress response of the fission yeast.
authors: Division of Life Sciences and Research Institute of Life Sciences, Kangwon National University, College of Natural Sciences, 192-1 Hyoja-2-dong, Chuncheon 200-701, Korea.
authors: Hye-Won
authors: HW
authors: Lim
authors: Su-Jung
authors: SJ
authors: Kim
authors: Eun-Hee
authors: EH
authors: Park
authors: Chang-Jin
authors: CJ
authors: Lim
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002104:Cadmium / Q000494:pharmacology; D020169:Caspases / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008969:Molecular Sequence Data; D009599:Nitroprusside / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D012333:RNA, Messenger; D029702:Schizosaccharomyces pombe Proteins; D002104:Cadmium; D009599:Nitroprusside; D006861:Hydrogen Peroxide; D020169:Caspases