title: Gene profiling for studying the mechanism of aflatoxin biosynthesis in Aspergillus flavus and A. parasiticus.
abstract: Aflatoxins are toxic and carcinogenic polyketide metabolites produced by certain fungal species, including Aspergillus flavus and A. parasiticus. Many internal and external factors, such as nutrition and environment affect aflatoxin biosynthesis; therefore, we analyzed the transcriptome of A. flavus using expressed sequence tags (ESTs) from a normalized cDNA expression library constructed from mycelia harvested under several conditions. A total of 7218 unique ESTs were identified from 26,110 sequenced cDNA clones. Functional classifications were assigned to these ESTs and genes, potentially involved in the aflatoxin contamination process, were identified. Based on this EST sequence information, a genomic DNA amplicon microarray was constructed at The Institute for Genomic Research (TIGR). To identify potential regulatory networks controlling aflatoxin contamination in food and feeds, gene expression profiles in aflatoxin-supportive media versus non-aflatoxin-supportive media were evaluated in A. flavus and A. parasiticus. Genes consistently expressed in several aflatoxin-supportive media are reported.
authors: USDA/ARS, Southern Regional Research Center, New Orleans, LA 70124, USA. jiuyu@srrc.ars.usda.gov
authors: Jiujiang
authors: J
authors: Yu
authors: Catherine M
authors: CM
authors: Ronning
authors: Jeffery R
authors: JR
authors: Wilkinson
authors: Bruce C
authors: BC
authors: Campbell
authors: Gary A
authors: GA
authors: Payne
authors: Deepak
authors: D
authors: Bhatnagar
authors: Thomas E
authors: TE
authors: Cleveland
authors: William C
authors: WC
authors: Nierman
mesh_terms: D000348:Aflatoxins / Q000096:biosynthesis* / Q000235:genetics; D001230:Aspergillus / Q000235:genetics* / Q000472:pathogenicity; D001231:Aspergillus flavus / Q000235:genetics / Q000472:pathogenicity; D018556:Crops, Agricultural; D020224:Expressed Sequence Tags / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D005800:Genes, Fungal; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000348:Aflatoxins; D037521:Virulence Factors