Effect of antimicrobial compounds on Balamuthia mandrillaris encystment and human brain microvascular endothelial cell cytopathogenicity.
Cycloheximide, ketoconazole, or preexposure of organisms to cytochalasin D prevented Balamuthia mandrillaris-associated cytopathogenicity in human brain microvascular endothelial cells, which constitute the blood-brain barrier. In an assay for inhibition of cyst production, these three agents prevented the production of cysts, suggesting that the biosynthesis of proteins and ergosterol and the polymerization of actin are important in cytopathogenicity and encystment.
Siddiqui
Ruqaiyyah
R
School of Biological and Chemical Sciences, Birkbeck College, University of London, London WC1E 7HX, England, United Kingdom.
Matin
Abdul
A
Warhurst
David
D
Stins
Monique
M
Khan
Naveed Ahmed
NA
D000199:Actins / Q000378:metabolism; D000563:Amebicides / Q000494:pharmacology*; D000656:Amoeba / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000469:parasitology; D004875:Ergosterol / Q000096:biosynthesis; D006801:Humans; D007654:Ketoconazole / Q000494:pharmacology
D000199:Actins; D000563:Amebicides; D015638:Cytochalasin D; D003513:Cycloheximide; D007654:Ketoconazole; D004875:Ergosterol