title: Long-term maintenance of a transgenic Catharanthus roseus hairy root line.
abstract: Stably transformed transgenic hairy root cultures have the potential to be a valuable production platform for a variety of secondary metabolites. This study reports that a transgenic hairy root culture of Catharanthus roseus has been stably maintained for over 4.5 years. This culture carries a transgene that expresses the green fluorescent protein under the control of the glucocorticoid-inducible promoter. Genomic PCR confirmed the presence of the GFP insert within the hairy roots, and induction with dexamethasone caused a significant (p < 0.02) increase in GFP levels.
authors: Department of Bioengineering, Rice University, Houston, Texas 77005, USA.
authors: Christie A M
authors: CA
authors: Peebles
authors: Susan I
authors: SI
authors: Gibson
authors: Jacqueline V
authors: JV
authors: Shanks
authors: Ka-Yiu
authors: KY
authors: San
mesh_terms: D030008:Catharanthus / Q000235:genetics* / Q000254:growth & development; D049452:Green Fluorescent Proteins / Q000235:genetics; D018517:Plant Roots / Q000235:genetics; D030821:Plants, Genetically Modified / Q000254:growth & development*; D016133:Polymerase Chain Reaction
chemical_list: D049452:Green Fluorescent Proteins