title: Comparison of two-phase lipase-catalyzed esterification on micro and bench scale.
abstract: Lipase type B from Candida antarctica was used to catalyze the esterification of propionic acid and 1-butanol in a water/n-decane two-phase system on micro and on bench scale. The reaction was described by a Ping Pong Bi Bi mechanism with alcohol inhibition. The kinetic parameters on micro and bench scale were compared; no significant differences were found. Furthermore, effects of temperature on activation and inactivation of the enzyme were found to be similar on micro and bench scale. Therefore, parameters found on either scale can be used for the other scale. Enzyme kinetic parameters can be determined on a micro scale, with very low consumption of reagents and catalyst, and then be applied to bench scale. This can reduce the cost of optimizing enzyme processes by downscaling.
authors: Wageningen University and Research Centre, Food and Bioprocess Engineering Group, Bomenweg 2, 6703 HD Wageningen, The Netherlands. jan.swarts@wur.nl
authors: Jan W
authors: JW
authors: Swarts
authors: Petra
authors: P
authors: Vossenberg
authors: Marieke H
authors: MH
authors: Meerman
authors: Anja E M
authors: AE
authors: Janssen
authors: Remko M
authors: RM
authors: Boom
mesh_terms: D020001:1-Butanol / Q000737:chemistry*; D000473:Alkanes / Q000737:chemistry*; D003198:Computer Simulation; D004789:Enzyme Activation; D004951:Esterification; D005656:Fungal Proteins; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D008956:Models, Chemical; D044367:Phase Transition; D010865:Pilot Projects; D011422:Propionates / Q000737:chemistry*; D013696:Temperature; D014867:Water / Q000737:chemistry*
chemical_list: D000473:Alkanes; D005656:Fungal Proteins; D011422:Propionates; D014867:Water; D020001:1-Butanol; D008049:Lipase; C431349:lipase B, Candida antarctica; C029658:propionic acid; C012867:decane