Selective transport of staphylococcal enterotoxin A through in vitro generated human M cells.
Staphylococcal enterotoxins are responsible for food poisoning and toxic shock syndrome due to their superantigen activity on T cells. Although their activity necessarily involves passage through the intestinal epithelium, little is known about this critical step. In the present study, we compared the in vitro transport of staphylococcal enterotoxin A through human intestinal absorptive and M cells. We found that the transport of the toxin through M cells was polarized and temperature-sensitive, in contrast with the less efficient transport of the toxin by absorptive cells. These data suggest the involvement of M cells in the intestinal absorption of staphylococcal enterotoxins.
Maresca
Marc
M
Laboratoire des Interactions Moléculaires et Systèmes Membranaires (IMSM), Université Paul Cézanne, Faculté des Sciences et Techniques de Saint-Jérôme, Avenue Escadrille Normandie-Niemen, 13397 Marseille Cedex 20, France. m.maresca@univ-cezanne.fr
Dumay
Eliane
E
Fantini
Jacques
J
Caporiccio
Bertrand
B
D001402:B-Lymphocytes / Q000378:metabolism*; D001692:Biological Transport; D018938:Caco-2 Cells; D002460:Cell Line; D016764:Cell Polarity; D018920:Coculture Techniques; D004768:Enterotoxins / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology; D007700:Kinetics; D013696:Temperature
D004768:Enterotoxins; C026066:enterotoxin A, Staphylococcal