title: Determination of glyoxal and methylglyoxal in environmental and biological matrices by stir bar sorptive extraction with in-situ derivatization.
abstract: Stir bar sorptive extraction with in-situ derivatization using 2,3-diaminonaphthalene (DAN) followed by liquid desorption and high performance liquid chromatography with diode array detection (SBSE(DAN)in-situ-LD-HPLC-DAD) was developed for the determination of glyoxal (Gly) and methylglyoxal (MGly) in environmental and biological matrices. DAN proved very good specificity as in-situ derivatising agent for Gly and MGly in aqueous media, allowing the formation of adducts with remarkable sensitivity, selectivity and the absence of photodegradation. Assays performed on spiked (1.0 microg L(-1)) water samples, under convenient experimental conditions, yielded recoveries of 96.2+/-7.9% for Gly and 96.1+/-6.4% for MGly. The analytical performance showed good accuracy, suitable precision (<12.0%), low detection limits (15 ng L(-1) for Gly and 25 ng L(-1) for MGly adducts) and excellent linear dynamic ranges (r2>0.99) from 0.1 to 120.0 microg L(-1). By using the standard addition method, the application of the present method to tap and swimming-pool water, beer, yeast cells suspension and urine samples allowed very good performance at the trace level. The proposed methodology proved to be a feasible alternative for routine quality control analysis, showing to be easy to implement, reliable, sensitive and with a low sample volume requirement to monitor Gly and MGly in environmental and biological matrices.
authors: University of Lisbon, Faculty of Sciences, Chemistry and Biochemistry Department, Campo Grande Ed. C8, 1749-016 Lisbon, Portugal.
authors: N R
authors: NR
authors: Neng
authors: C A A
authors: CA
authors: Cordeiro
authors: A P
authors: AP
authors: Freire
authors: J M F
authors: JM
authors: Nogueira
mesh_terms: D015081:2-Naphthylamine / Q000031:analogs & derivatives / Q000737:chemistry; D000327:Adsorption; D001515:Beer / Q000032:analysis; D005591:Chemical Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004784:Environmental Monitoring / Q000379:methods; D005240:Feasibility Studies; D005506:Food Contamination / Q000032:analysis; D005618:Fresh Water / Q000032:analysis; D006037:Glyoxal / Q000302:isolation & purification*; D011765:Pyruvaldehyde / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D014556:Urine / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014881:Water Supply / Q000032:analysis
chemical_list: D014874:Water Pollutants, Chemical; C026373:2,3-diaminonaphthalene; D006037:Glyoxal; D011765:Pyruvaldehyde; D015081:2-Naphthylamine