[Effect of small interfering RNA-induced LKB1 gene silencing on the biological behavior of lung carcinoma cells].
OBJECTIVE
To study the effect of the small interfering RNA (siRNA)-mediated LKB1 gene silencing on the biological behavior of lung carcinoma cells.


METHODS
RNA interference technique was used to silence LKB1 gene in lung carcinoma cells, and the expression of LKB1 protein were subsequently detected by Western blotting. The cell proliferation was then assessed by observing the growth curve and clone formation of the cells, and cell cycle and apoptosis were assayed by flow cytometry.


RESULTS
LKB1 siRNA resulted in remarkable suppression of LKB1 expression in the lung carcinoma cells. LKB1 gene silencing resulted in accelerated cell proliferation, but cell apoptosis underwent no significant changes.


CONCLUSIONS
Down-regulation of LKB1 gene expression can stimulate malignant biological behavior of lung carcinoma cells.
Liang
Xuan
X
Department of Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
Nan
Ke-jun
KJ
Xu
Qin-zhi
QZ
D000091162:AMP-Activated Protein Kinase Kinases; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005434:Flow Cytometry; D020868:Gene Silencing; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D017346:Protein Serine-Threonine Kinases / Q000172:deficiency* / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*
D034741:RNA, Small Interfering; D017346:Protein Serine-Threonine Kinases; C109790:STK11 protein, human; D000091162:AMP-Activated Protein Kinase Kinases