Phylogenetic comparison of the carboxy-terminal region of glycoprotein C (gC) of bovine herpesviruses (BoHV) 1.1, 1.2 and 5 from South America (SA).
Different types and subtypes of bovine herpesvirus 1 and 5 (BoHV-1 and BoHV-5) have been associated to different clinical conditions of cattle, in such a way that type/subtype differentiation has become an essential tool for understanding the pathogenesis and epidemiology of BoHV infections. In search for a genomic region that would allow a clear distinction between BoHV-1 and BoHV-5, the carboxy-terminal portion of glycoprotein C (gC), corresponding to residues 321-450 (BoHV-1) and 301-429 (BoHV-5) of 23 South American (SA) isolates (Brazil mostly) was amplified and sequenced. The nucleotide sequence alignments revealed levels of genomic similarity ranging from 98.7 to 99.8% among BoHV-1 isolates, 88.3 to 92% between BoHV-1/BoHV-5 and 96 to 99.7% among BoHV-5 isolates. At the amino acid level, sequence similarity varied ranging from 97.5 to 99.5% among BoHV-1, 77.5 to 84.4% between BoHV-1/BoHV-5 and 92.1 to 99.5% (BoHV-5/BoHV-5). The isolates could be clearly separated into BoHV-1.1, BoHV-1.2 and BoHV-5 after phylogenetic analysis. The results suggest that the phylogenetic analysis performed here can be used as a potential molecular epidemiological tool for herpesviruses.
Esteves
P A
PA
Embrapa Suínos e Aves, BR 153, Km 110, CEP: 89700-000, Concórdia, SC, Brazil. pesteves@cnpsa.embrapa.br
Dellagostin
O A
OA
Pinto
L S
LS
Silva
A D
AD
Spilki
F R
FR
Ciacci-Zanella
J R
JR
Hübner
S O
SO
Puentes
R
R
Maisonnave
J
J
Franco
A C
AC
Rijsewijk
F A M
FA
Batista
H B C R
HB
Teixeira
T F
TF
Dezen
D
D
Oliveira
A P
AP
David
C
C
Arns
C W
CW
Roehe
P M
PM
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000821:virology*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D025881:Herpesvirus 5, Bovine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D013020:South America / Q000453:epidemiology; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics
D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1