title: Endothelial nitric oxide synthase polymorphisms in biopsy-proven erythema nodosum from a defined population.
abstract: OBJECTIVE
  To assess the potential in-fluence of endothelial nitric oxide synthase (eNOS) polymorphisms in the susceptibility to and clinical expression of a series of patients diagnosed with biopsy-proven erythema nodosum (EN).
  
  
  METHODS
  Ninety-seven unselected patients from Northwest Spain with biopsy-proven EN were studied. Patients and ethnically matched controls were genotyped by PCR based techniques for a variable number tandem repeat polymorphism in intron 4, a T/C polymorphism at position -786 in the promoter region and a polymorphism in exon 7 (298Glu/Asp or 5557G/T) of the eNOS gene.
  
  
  RESULTS
  No differences in allele or genotype frequencies for any of the individual eNOS polymorphisms were observed between biopsy-proven patients with EN and controls. It was also the case when patients with EN secondary to sarcoidosis were compared with the remaining patients or controls. In the group of patients with EN, no linkage disequilibrium between these polymorphisms was found. Also, no significant differences in haplotype frequencies were observed between patients and controls.
  
  
  CONCLUSIONS
  Our present results do not support a role for eNOS polymorphisms in the susceptibility to and clinical expression of EN.
authors: The Centre for Integrated Genomic Medical Research, School of Epidemiology, and Health Sciences, the University of Manchester, Manchester, United Kingdom.
authors: M M
authors: MM
authors: Amoli
authors: H
authors: H
authors: Lopez-Agreda
authors: O
authors: O
authors: Suarez-Amor
authors: J
authors: J
authors: Martin
authors: W E R
authors: WE
authors: Ollier
authors: M A
authors: MA
authors: Gonzalez-Gay
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D004893:Erythema Nodosum / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D011110:Polymorphism, Genetic; D012507:Sarcoidosis / Q000235:genetics; D020080:Tandem Repeat Sequences
chemical_list: D052250:Nitric Oxide Synthase Type III