title: Novel mating type-dependent transcripts at the mating type locus in Magnaporthe oryzae.
abstract: The mating type locus (MAT1) of Magnaporthe oryzae has similar structural organization to MAT in other ascomycetes and encodes the mating type genes MAT1-1-1 with an alpha-box motif and MAT1-2-1 with an HMG-box motif in the MAT1-1 and MAT1-2 idiomorphs, respectively. Sequence and expression analyses of the MAT1 locus indicated a second open reading frame (ORF), MAT1-1-2, in the MAT1-1 idiomorph, and novel mating-type dependent ORFs (MAT1-1-3 and MAT1-2-2) at the locus. The MAT1-1-3 ORF initiated within the MAT1-1 idiomorph while the MAT1-2-2 ORF initiated at the border of the MAT1-2 idiomorph with both ORFs sharing most of their reading frames in the MAT1 flanking region. This suggests that the encoded proteins (MAT1-1-3 and MAT1-2-2) should be similar in their primary structures but can be distinguished by distinct N-termini with amino acids of 1 and 32, respectively, in each mating type. A CT dinucleotide repeat, (CT)n, present in the upstream region of MAT1-1-3, was polymorphic among the isolates.
authors: Laboratory of Plant Pathology, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Tokyo 183-8509, Japan.
authors: Masaki
authors: M
authors: Kanamori
authors: Hana
authors: H
authors: Kato
authors: Nobuko
authors: N
authors: Yasuda
authors: Shinzo
authors: S
authors: Koizumi
authors: Tobin L
authors: TL
authors: Peever
authors: Takashi
authors: T
authors: Kamakura
authors: Tohru
authors: T
authors: Teraoka
authors: Tsutomu
authors: T
authors: Arie
mesh_terms: D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D020029:Base Pairing; D001483:Base Sequence; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D018900:Dinucleotide Repeats; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D024742:HMG-Box Domains; D020082:Magnaporthe / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D020121:5' Untranslated Regions; D017931:DNA Primers; D004271:DNA, Fungal; D005656:Fungal Proteins; D012333:RNA, Messenger