Insulating tethered bilayer lipid membranes to study membrane proteins.
Tethered bilayer lipid membranes are stable and promising model systems that mimic several properties of biological membranes. They provide an electrically insulating platform for the incorporation and study of functional membrane proteins, especially ion channels. Covalently linked to a solid support, they also offer enhanced stability compared with other model architectures. If the support can be used as an electrode, electrical characterisation of the system is possible and biosensing applications can be envisioned.Here, we will review some tethered bilayer structures developed in the past and show some examples of functional protein incorporation, both on oxide and gold substrates.
Köper
Ingo
I
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany. ingo.koeper@mpip-mainz.mpg.de
D004563:Electrochemistry; D004566:Electrodes; D006046:Gold; D007473:Ion Channels / Q000737:chemistry / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008954:Models, Biological; D015394:Molecular Structure; D017655:Silicon Compounds
D007473:Ion Channels; D008051:Lipid Bilayers; D008565:Membrane Proteins; D017655:Silicon Compounds; D006046:Gold