Metacercarial proteins interacting with WD40-repeat protein of Clonorchis sinensis.
The WD40-repeat proteins serve as a platform coordinating partner proteins and are involved in a range of regulatory cellular functions. A WD40-repeat protein (CsWD1) of Clonorchis sinensis previously cloned is expressed stage-specifically in the tegumental syncytium of C. sinensis metacercariae. In the present study, interacting proteins with the CsWD1 protein was purified by immunoprecipitation and 2 dimension gel electrophoresis from the C. sinensis metacercaria soluble extract, and tryptic peptides were analyzed by LC/ESI-MS. Putative partner proteins were annotated to be actin-2, glyceraldehyde-3-phosphate dehydrogenase, and hypothetical and unmanned proteins. The CsWD1 protein was predicted to contain 3 conserved actin-interacting residues on its functional surface. With these results, the CsWD1 protein is suggested to be an actin-interacting protein of C. sinensis.
Cho
Pyo-Yun
PY
Department of Parasitology, Chung-Ang University College of Medicine, Seoul 156-756, Korea.
Kim
Tae-Im
TI
Li
Shunyu
S
Hong
Sung-Jong
SJ
Choi
Min-Ho
MH
Hong
Sung-Tae
ST
Chung
Yong -e
Y-
D000818:Animals; D000909:Antibodies, Helminth / Q000378:metabolism; D003004:Clonorchis sinensis / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D015801:Helminth Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000737:chemistry; D008840:Microfilament Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
D000909:Antibodies, Helminth; D015801:Helminth Proteins; D007074:Immunoglobulin G; D008840:Microfilament Proteins