title: [Genetic variations of membrane gene of infectious bronchitis virus strains isolated in China between 1995 and 2004].
abstract: Membrane (M) protein genes of 20 infectious bronchitis virus (IBV) strains isolated in China between 1995 and 2004 were sequenced and analyzed. The M genes of twenty isolates were composed of 672 to 681 nucleotides, encoding polypeptides of 223 to 226 amino acid residues. Variations of the deduced amino acids of M gene mainly occurred at positions 2 to 17 and 221 to 233, comparing with that of the IBV strain LX4. There were deletions or insertions in the M gene of Chinese isolates at amino acid position 2 to 6, leading to the loss or gain of a glycosylation site. Phylogenetic tree based on amino acid sequences of M genes from 20 Chinese isolates and 34 reference strains showed that they were classified into five distinct clusters. Most of the Chinese IBV strains were included in clusters II and IV, forming distinct groups. The isolates in cluster II showed a close evolutionary relationship with Taiwan isolates. Furthermore, recombination especially the recombination between field isolates and vaccine strains had been observed while comparing the phylogeny of M genes with those of S1 and N genes.
authors: Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology Harbin Veterinary Researoh Institute, CAAS, Harbin 150001, Heilongjiang, China.
authors: Lei
authors: L
authors: Nie
authors: Qing-xia
authors: QX
authors: Zhang
authors: Zong-xi
authors: ZX
authors: Han
authors: Yu-hao
authors: YH
authors: Shao
authors: Jun-gong
authors: JG
authors: Rong
authors: Sheng-wang
authors: SW
authors: Liu
authors: Xian-gang
authors: XG
authors: Kong
mesh_terms: D000595:Amino Acid Sequence; D002681:China; D014644:Genetic Variation; D001351:Infectious bronchitis virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014763:Viral Matrix Proteins / Q000235:genetics*
chemical_list: D014763:Viral Matrix Proteins