Semliki Forest virus vectors expressing the H and HN genes of measles and mumps viruses reduce immunity induced by the envelope protein genes of rubella virus.
A Semliki Forest virus (SFV) recombinant particle vaccine vector was constructed expressing the viral E1 and E2 envelope proteins of the RA27/3 vaccine strain of rubella virus. This vector induced high titres of antibody after intramuscular administration to Balb/C mice, both following initial vaccination and a boost 4 weeks later. This occurred for antibody as measured by ELISA and as measured by a latex agglutination test. However, co-administration of similar particles expressing the measles virus H protein and the mumps virus HN protein with the rubella protein expressing vector resulted in reduction of the anti-rubella immune response.
Callagy
Sara J
SJ
Virus Group, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland.
Kelly
Barbara J
BJ
Fleeton
Marina N
MN
Sheahan
Brian J
BJ
Galbraith
Sareen E
SE
Atkins
Gregory J
GJ
D000372:Agglutination Tests; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics*; D007273:Injections, Intramuscular; D008459:Measles virus / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D009109:Mumps virus / Q000235:genetics*; D012412:Rubella virus / Q000235:genetics*; D012672:Semliki forest virus / Q000235:genetics; D014611:Vaccination / Q000379:methods; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014759:Viral Envelope Proteins