Application of capillary electrophoresis to study phenolic profiles of honeybee-collected pollen.
Honeybee-collected pollen is promoted as a health food with a wide range of nutritional and therapeutic properties. A high-performance capillary electrophoresis with amperometric detection method has been developed for the simultaneous determination of bioactive ingredients in 10 samples of honeybee-collected pollen in this work. Under the optimum conditions, 13 phenolic components can be well-separated or nearly baseline-separated (apigenin and vanillic acid peaks) within 29 min at the separation voltage of 14 kV in a 50 mM borax running buffer (pH 9.0), and adequate extraction was obtained with ethanol for the determination of the above 13 compounds. Recovery (94.1-104.0%), repeatability of the peak current (<5.4%), and detection limits (6.9 x 10(-7)-6.4 x 10(-9) g mL(-1)) for the method were evaluated. This procedure was successfully used for the analysis and comparison of the phenolic content of honeybee-collected pollen samples originating from different floral origins based on their electropherograms or "phenolic profiles".
Chu
Qingcui
Q
Department of Chemistry, East China Normal University, Shanghai 200062, China.
Tian
Xiuhui
X
Jiang
Lianmei
L
Ye
Jiannong
J
D000818:Animals; D001516:Bees; D019075:Electrophoresis, Capillary / Q000379:methods*; D010636:Phenols / Q000032:analysis*; D011058:Pollen / Q000737:chemistry*; D015203:Reproducibility of Results
D010636:Phenols