title: Prevalence and natural host range of Homalodisca coagulata virus-1 (HoCV-1).
abstract: Transmission electron microscopy was used to confirm the presence of picorna-like virus particles presumed to be Homalodisca coagulata virus-1 (HoCV-1) in the midgut region of adult glassy-winged sharpshooters (GWSS). In addition, we offer a reverse transcription polymerase chain reaction (RT-PCR) assay for the detection of this virus with a sensitivity of approximately 95 genome equivalents. A survey employing this assay in conjunction with GWSS samples collected throughout the United States including California, Hawaii, Florida Georgia, and the Carolinas revealed a fairly widespread pattern of distribution, although potentially restricted to temperate regions, areas with elevated host densities, or to populations of a common origin. The virus was found to naturally infect adults regardless of host plant and was not specific to a particular lifestage or sex. Examination of alternate leafhopper species further demonstrated that, although infection is not ubiquitous to all sharpshooter genera, HoCV-1 is not limited to Homalodisca vitripennis (=H. coagulata).
authors: USDA ARS US Horticultural Research Laboratory, Ft. Pierce, FL 34945, USA.
authors: L E
authors: LE
authors: Hunnicutt
authors: J
authors: J
authors: Mozoruk
authors: W B
authors: WB
authors: Hunter
authors: J M
authors: JM
authors: Crosslin
authors: R D
authors: RD
authors: Cave
authors: C A
authors: CA
authors: Powell
mesh_terms: D000818:Animals; D006430:Hemiptera / Q000821:virology*; D007304:Insect Viruses / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D046529:Microscopy, Electron, Transmission; D015995:Prevalence; D012328:RNA Viruses / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D020133:Reverse Transcriptase Polymerase Chain Reaction