Peculiarities of the anticoagulant properties for the newly synthesized preparations of coumarin-related compounds.
A) OBJECTIVES: Investigations of the four synthetic coumarin-related compounds: N' - allylthiouridine-3-carbamoil coumarin (GSH-16), N'- morpholylthiouridine-3-carbamoil coumarin (GSH-17), N'-O-fluor-benzyl-N'-3-carbamoil piperaside coumarin (GSH-10) and 6-nitroallylamide-3-carboxy coumarin (GSH-84), were done for study of their role in the processes of hemocoagulation. B) DESIGN AND METHODS: Investigations were carried out on 120 white rats (180-200g), which were injected intravenously by 0.5 ml and 1.0 ml of 1 % solution of GSH-17 and decapitated after 10 and 30 min after the injection by use of light chloroform narcosis. Separation of the liver was done by simultaneous washing with cold physiological solution. C) RESULTS: It was shown that the hemostabilization action of GSH-17 is highly dose- and time - dependent, with by the pronounced decrease of rat liver thromboplastic activity after 10 and especially 30 min following intravenous injection. D) CONCLUSION: One of the possible mechanisms for haemostatic effect of the studied preparations, particularly GSH-17, probably can be accepted their effect on metabolism of arachidonic acid by lipoxygenase and cycloxygenase mechanisms [9, 10]. The results of this investigations have not only the academic interest, but they have also a significant importance for definite branches of practical medicine as a very effective blood stabilizing factors.
Ghazaryan
Artyom V
AV
Institute of Molecular Biology of the National Academy of Sciences, The Republic of Armenia.
Khatchadourian
Armen S
AS
Karagyozyan
Maria K
MK
Kachatryan
Anahit R
AR
Sekoyan
Eduard S
ES
Bdoyan
Hovsep K
HK
Melkumyan
Hakob V
HV
Karageuzyan
Konstantin G
KG
D000818:Animals; D000925:Anticoagulants / Q000138:chemical synthesis* / Q000494:pharmacology*; D003374:Coumarins / Q000138:chemical synthesis* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D011517:Prothrombin Time; D051381:Rats; D013925:Thromboplastin / Q000096:biosynthesis; D013997:Time Factors
D000925:Anticoagulants; D003374:Coumarins; D013925:Thromboplastin