Activity-based protein profiling for the functional annotation of enzymes.
Activity-based protein profiling (ABPP), the use of active site-directed chemical probes to monitor enzyme function in complex biological systems, is emerging as a powerful post-genomic technology. ABPP probes have been developed for several enzyme classes and have been used to inventory enzyme activities en masse for a range of (patho) physiological processes. By presenting specific examples, we show here that ABPP provides researchers with a distinctive set of chemical tools to embark on the assignment of functions to many of the uncharacterized enzymes that populate eukaryotic and prokaryotic proteomes.
Barglow
Katherine T
KT
The Skaggs Institute for Chemical Biology and Department of Chemical Physiology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California 92037, USA.
Cravatt
Benjamin F
BF
D000345:Affinity Labels; D000818:Animals; D001665:Binding Sites / Q000502:physiology; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry; D015335:Molecular Probes; D040901:Proteomics / Q000379:methods* / Q000639:trends; D002787:Sterol Esterase; D013379:Substrate Specificity
D000345:Affinity Labels; D004798:Enzymes; D015335:Molecular Probes; D002265:Carboxylic Ester Hydrolases; C515677:NCEH1 protein, human; D002787:Sterol Esterase