title: Two Cys residues essential for von Willebrand factor multimer assembly in the Golgi.
abstract: Von Willebrand factor (VWF) dimerizes through C-terminal CK domains, and VWF dimers assemble into multimers in the Golgi by forming intersubunit disulfide bonds between D3 domains. This unusual oxidoreductase reaction requires the VWF propeptide (domains D1D2), which acts as an endogenous pH-dependent chaperone. The cysteines involved in multimer assembly were characterized by using a VWF construct that encodes the N-terminal D1D2D'D3 domains. Modification with thiol-specific reagents demonstrated that secreted D'D3 monomer contained reduced Cys, whereas D'D3 dimer and propeptide did not. Reduced Cys in the D'D3 monomer were alkylated with N-ethylmaleimide and analyzed by mass spectrometry. All 52 Cys within the D'D3 region were observed, and only Cys(1099) and Cys(1142) were modified by N-ethylmaleimide. When introduced into the D1D2D'D3 construct, the mutation C1099A or C1142A markedly impaired the formation of D'D3 dimers, and the double mutation prevented dimerization. In full-length VWF, the mutations C1099A and C1099A/C1142A prevented multimer assembly; the mutation C1142A allowed the formation of almost exclusively dimers, with few tetramers and no multimers larger than hexamers. Therefore, Cys(1099) and Cys(1142) are essential for the oxidoreductase mechanism of VWF multimerization. Cys(1142) is reported to form a Cys(1142)-Cys(1142) intersubunit bond, suggesting that Cys(1099) also participates in a Cys(1099)-Cys(1099) disulfide bond between D3 domains. This arrangement of intersubunit disulfide bonds implies that the dimeric N-terminal D'D3 domains of VWF subunits align in a parallel orientation within VWF multimers.
authors: Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors: Angie R
authors: AR
authors: Purvis
authors: Julia
authors: J
authors: Gross
authors: Luke T
authors: LT
authors: Dang
authors: Ren-Huai
authors: RH
authors: Huang
authors: Milan
authors: M
authors: Kapadia
authors: R Reid
authors: RR
authors: Townsend
authors: J Evan
authors: JE
authors: Sadler
mesh_terms: D000818:Animals; D000941:Antigens / Q000096:biosynthesis* / Q000737:chemistry; D002460:Cell Line; D006224:Cricetinae; D003545:Cysteine; D003553:Cystine; D019281:Dimerization; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D007668:Kidney; D016297:Mutagenesis, Site-Directed; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D014841:von Willebrand Factor / Q000276:immunology
chemical_list: D000941:Antigens; D010455:Peptides; D021122:Protein Subunits; D011994:Recombinant Proteins; D013438:Sulfhydryl Compounds; C029417:Von Willebrand antigen; D014841:von Willebrand Factor; D003553:Cystine; D003545:Cysteine