Dental pulp stem cells bioadhesivity: evaluation on mineral-trioxide-aggregate.
Stem cells are undifferentiated cells that have the capacity to self-renew. They have been discovered in many adult tissues, including teeth. Dental Pulp Mesenchymal Stem Cells (DP-MSCs) are involved in dental repair by activation of growth factors, released after caries and have the ability to regenerate a dentin-pulp-like complex. The molecular/cellular research gives the possibility to grow new tissues and biological structures for clinical applications, providing cells for therapies including cell transplantation and tissue engineering. In this study DP-MSCs were derived from dental pulp of 10 donors. To evaluate material toxicity, after in vitro isolation, the cells were seeded on mineral trioxide aggregate (MTA). Initial light microscopy investigation of cells revealed no signs of cell death due to toxicity or infection, on the contrary the scaffolds supplied an excellent support for cell structures, the cells proliferated and adhered to substrate. Similar observation was seen in scanning electron microscopy, in particular the cells had proliferated and spread, covering a considerable part of the surface of the biomaterials investigated, with an elaborate form of attachment, in fact, the cells formed a continuous layer on the upper surface of the MTA. In conclusion, the aim of this study is to demonstrate that DP-MSCs combined with MTA could be a potential source for regenerative medicine, encouraging further study to evaluate the new dentin formation.
Trubiani
O
O
Department of Stomatology and Oral Sciences, University of Chieti-Pescara, Chieti, Italy. trubiani@unich.it
D'Arcangelo
C
C
Di Iorio
D
D
Di Nardo Di Maio
F
F
Caputi
S
S
D000328:Adult; D017607:Aluminum Compounds / Q000494:pharmacology*; D017610:Calcium Compounds / Q000494:pharmacology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D004338:Drug Combinations; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000494:pharmacology*; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*
D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate