Involvement of insulin-like growth factor-binding protein-related protein 1 in decidualization of human endometrial stromal cells.
Uterine decidualization is crucial for successful implantation and the establishment of pregnancy. In the present study, the expression of insulin-like growth factor-binding protein (IGFBP)-related protein 1 (IGFBP-rP1) in the human uterus and endometrial stromal cells (ESCs) and its physiological significance in decidualization were examined. IGFBP-rP1 protein was localized in the glandular epithelium and stromal cells, and blood vessels in the endometrium. Cultured stromal cells expressed IGFBP-rP1 and secreted it into the medium. IGFBP-rP1 was localized mostly in the cytoplasm near the nucleus. Knocking down the endogenous IGFBP-rP1 expression in stromal cells, by a small interfering (si)RNA, diminished the expression of prolactin and IGFBP-1 which serve as decidual markers. These results suggest that IGFBP-rP1 may play a role in decidualization of ESCs.
Kutsukake
Masahiko
M
Department of Endocrine Pharmacology, Tokyo University of Pharmacy and Life Sciences, Hachioji-Shi, Tokyo, Japan.
Ishihara
Ryosuke
R
Yoshie
Mikihiro
M
Kogo
Hiroshi
H
Tamura
Kazuhiro
K
D000328:Adult; D015153:Blotting, Western; D002478:Cells, Cultured; D003656:Decidua / Q000378:metabolism*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D018969:Insulin-Like Growth Factor Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D011388:Prolactin / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism
D018970:Insulin-Like Growth Factor Binding Protein 1; D018969:Insulin-Like Growth Factor Binding Proteins; D034741:RNA, Small Interfering; C102529:insulin-like growth factor binding protein-related protein 1; D011388:Prolactin