title: Genetic analysis of the DBC2 gene in gastric cancer.
abstract: The DBC2 (Deleted in breast cancer, RhoBTB2) has been identified as a tumor suppressor gene that has growth inhibitory function. To investigate whether genetic alterations of the DBC2 gene are involved in the development of gastric cancer, we analyzed mutations and allelic loss in the DBC2 gene in 95 primary gastric cancers by PCR-SSCP, sequencing and LOH analysis. In the mutational analysis, we found one missense somatic mutation (CGG-->TGG, R275W) in the BTB/POZ domain of the gene in a patient with advanced gastric cancer and lymph node metastasis. In addition, we found one known polymorphism and three novel polymorphisms in the coding region of DBC2, which showed an amino acid change, and was detected in both the cancer cells and corresponding normal cells. On LOH analysis, 62 cases were heterozygous for at least one marker and 18 cases (29.0%) showed allelic loss at these markers. In conclusion, the mutations and allelic loss in the DBC2 gene are uncommon in gastric cancers in Korean patients. Further studies to identify the target gene at 8q21 responsible for the development of gastric cancer should be explored.
authors: Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Yong Gu
authors: YG
authors: Cho
authors: Byung Joon
authors: BJ
authors: Choi
authors: Chang Jae
authors: CJ
authors: Kim
authors: Jae Hwi
authors: JH
authors: Song
authors: Cao
authors: C
authors: Zhang
authors: Suk Woo
authors: SW
authors: Nam
authors: Jung Young
authors: JY
authors: Lee
authors: Won Sang
authors: WS
authors: Park
mesh_terms: D000368:Aged; D002454:Cell Differentiation; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007723:Korea / Q000453:epidemiology; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D009363:Neoplasm Proteins / Q000235:genetics* / Q000502:physiology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000502:physiology
chemical_list: D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; C497336:RHOBTB2 protein, human; D025521:Tumor Suppressor Proteins; D019204:GTP-Binding Proteins