[Study of diphtheria anatoxins in immunochemical and tissue culture assays].
Equine diphtheria antitoxins from different manufacturers were studied. Their immunochemical interaction with diphtheria toxin, toxoid, and antigens of Corynebacterium diphtheriae in ELISA and immunoblotting assays as well as biological activity in CHO cell assay were compared. The discovered differences between antitoxin samples with stated equal activity in IU/ml point to heterogeneity of antigen composition in preparations used for immunization. Mentioned methods allow to standardize antitoxins basing on their biological activity and immunochemical characteristics.
Gal'vidis
I A
IA
Sviridov
V V
VV
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D015153:Blotting, Western; D016466:CHO Cells; D003353:Corynebacterium diphtheriae / Q000276:immunology; D006224:Cricetinae; D003412:Cricetulus; D004166:Diphtheria Antitoxin / Q000032:analysis* / Q000276:immunology / Q000633:toxicity; D004167:Diphtheria Toxin / Q000276:immunology; D004168:Diphtheria Toxoid / Q000276:immunology; D023381:Endpoint Determination; D004797:Enzyme-Linked Immunosorbent Assay; D006736:Horses; D007106:Immune Sera / Q000032:analysis* / Q000276:immunology / Q000633:toxicity; D015203:Reproducibility of Results
D000942:Antigens, Bacterial; D004166:Diphtheria Antitoxin; D004167:Diphtheria Toxin; D004168:Diphtheria Toxoid; D007106:Immune Sera