An epigenetic modifier results in improved in vitro blastocyst production after somatic cell nuclear transfer.
The present study was designed to examine the effect of trichostatin A (TSA), an inhibitor of histone deacetylase, on development of porcine cloned embryos. Our results showed that treatment of cloned embryos derived from sow oocytes with 50 nM TSA for up to 24 h after the onset of activation could significantly improve blastocyst yield compared to the control (46.4+/-4.6% vs 17.7+/-4.9% for treated and untreated embryos, respectively; p<0.05), whereas similar cleavage rate and total cell number per blastocyst were observed. In order to assess if the improvement is cell line specific, three cell lines were tested, and for all cell lines an enhancement in blastocyst development compared to their corresponding control was observed. Our data demonstrate that TSA treatment after somatic cell nuclear transfer in the pig can significantly improve the in vitro blastocyst production.
Zhang
Yunhai
Y
Section of Population Genetics and Embryology, Department of Genetic and Biotechnology, Danish Institute of Agricultural Sciences, Tjele, Denmark. yunhaizhang2005@gmail.com
Li
Juan
J
Villemoes
Klaus
K
Pedersen
Anette M
AM
Purup
Stig
S
Vajta
Gábor
G
D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000379:methods*; D044127:Epigenesis, Genetic; D005260:Female; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013552:Swine
D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; C012589:trichostatin A; D006655:Histone Deacetylases