Insulin releasing properties of the temporin family of antimicrobial peptides.
Temporin-1Vb, -1Oe, -1DRb, and -1TGb (10(-8) -10(-6)M) produced significant (p<0.05) and concentration-dependent stimulatory effects on insulin secretion from clonal rat BRIN-BD11 cells without increased release of lactate dehydrogenase. Temporin-1Va and temporin-1Vc (10(-8) - 10(-6)M) also stimulated insulin-release but were cytotoxic at 10(-6)M. Temporin-1DRa was without effect. The temporins at 10(-7) M had no effect on intracellular calcium concentrations suggesting that they stimulate insulin release via a K(ATP) channel- independent pathway.
Abdel-Wahab
Yasser H A
YH
Department of Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, 17666 Al-Ain, UAE.
Marenah
Lamin
L
Flatt
Peter R
PR
Conlon
J Michael
JM
D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D007328:Insulin / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization
D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D007328:Insulin; D010455:Peptides; D011506:Proteins; C104501:temporin; D002118:Calcium