BALB/c mice resistant to Toxoplasma gondii infection proved to be highly susceptible when previously infected with Myocoptes musculinus fur mites.
The immune response induced by Toxoplasma gondii is characterized by Th1 immune mechanisms. We previously demonstrated that C57BL/6 mice infested with Myocoptes musculinus and infected with T. gondii by intraperitoneal route undergo accelerated mortality according to Th2 immune mechanisms induced by the acarian. To evaluate whether infection with M. musculinus influences T. gondii-induced Th1 response in a resistant mouse lineage, BALB/c, which develops latent chronic toxoplasmosis in a way similar to that observed in immunocompetent humans, this study was done. The animals were infected with T. gondii ME-49 strain 1 month after M. musculinus infestation, being the survival and the immune response monitored. The double-infected displayed higher mortality rate if compared with the mono-infected mice. In addition, infection with M. musculinus changed the T. gondii-specific immune response, converting BALB/c host to a susceptible phenotype. Spleen cells had increased the levels of IL-4 in double-infected mice. This alteration was associated with severe pneumonia, encephalitis and wasting condition. In addition, a higher tissue parasitism was observed in double-infected animals. It can be concluded that infection with these two contrasting parasites, M. musculinus and T. gondii, may convert an immunocompetent host into a susceptible one, and such a host will develop severe toxoplasmosis.
Welter
Aurea
A
Laboratory of Immunoparasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil.
Mineo
José Roberto
JR
de Oliveira Silva
Deise Aparecida
DA
Lourenço
Elaine Vicente
EV
Vieira Ferro
Eloísa Amália
EA
Roque-Barreira
Maria Cristina
MC
Maria da Silva
Neide
N
D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002490:Central Nervous System / Q000469:parasitology; D004198:Disease Susceptibility; D004804:Eosinophils / Q000276:immunology; D005260:Female; D006790:Host-Parasite Interactions; D007113:Immunity, Innate; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007150:Immunohistochemistry; D016753:Interleukin-10 / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D015848:Interleukin-5 / Q000276:immunology; D007958:Leukocyte Count; D008168:Lung / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008924:Mite Infestations / Q000150:complications / Q000276:immunology* / Q000401:mortality; D008925:Mites; D012867:Skin / Q000469:parasitology; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014122:Toxoplasma / Q000302:isolation & purification; D014123:Toxoplasmosis / Q000150:complications / Q000276:immunology* / Q000401:mortality; D014124:Toxoplasmosis, Animal / Q000150:complications / Q000276:immunology* / Q000401:mortality
D000953:Antigens, Protozoan; D015415:Biomarkers; D007074:Immunoglobulin G; D015848:Interleukin-5; D016753:Interleukin-10; D015847:Interleukin-4; D007073:Immunoglobulin E