Enolases from Gram-positive bacterial pathogens and commensal lactobacilli share functional similarity in virulence-associated traits.
Enolase occurs as a cytoplasmic and a surface-associated protein in bacteria. Enolases of the bacterial pathogens Streptococcus pyogenes, Streptococcus pneumoniae and Staphylococcus aureus, as well as of the commensal lactic acid bacteria, Lactobacillus crispatus and Lactobacillus johnsonii, were purified as His(6)-fusion proteins from recombinant Escherichia coli. The fusion proteins were compared for putative virulence-associated functions, i.e., binding of human plasminogen, enhancement of plasminogen activation by human plasminogen activators, as well as binding to immobilized laminin, fibronectin and collagens. The individual enolases showed varying efficiencies in these functions. In particular, highly and equally effective interactions with plasminogen and laminin were seen with lactobacillar and staphylococcal enolases.
Antikainen
Jenni
J
General Microbiology, Faculty of Biosciences, University of Helsinki, Helsinki, Finland.
Kuparinen
Veera
V
Lähteenmäki
Kaarina
K
Korhonen
Timo K
TK
D002648:Child; D003001:Cloning, Molecular; D003094:Collagen / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D007778:Lactobacillus / Q000201:enzymology* / Q000235:genetics; D007797:Laminin / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D010802:Phylogeny; D010958:Plasminogen / Q000378:metabolism; D010960:Plasminogen Activators / Q000378:metabolism; D011485:Protein Binding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000235:genetics; D013296:Streptococcus pneumoniae / Q000201:enzymology* / Q000235:genetics; D013297:Streptococcus pyogenes / Q000201:enzymology* / Q000235:genetics
D004269:DNA, Bacterial; D005353:Fibronectins; D007797:Laminin; D011994:Recombinant Proteins; D010958:Plasminogen; D003094:Collagen; D010960:Plasminogen Activators; D010751:Phosphopyruvate Hydratase