Mode of antibacterial action of a signal peptide, Pep27 from Streptococcus pneumoniae.
The aim of this study was to assess the in vitro antimicrobial activity of Pep27 and its mode of action. The results indicated Pep27 exhibited antibacterial activities against pathogenic Gram-positive and Gram-negative bacteria without hemolytic effect on human erythrocytes, but it did not exhibit antifungal activity. To understand the mode of action of Pep27, the cellular distribution of the peptide was investigated. Flow cytometry analysis exhibited Pep27 penetrated the bacterial membrane by an energy-independent pathway without any damage to the membrane when examined using liposome and membrane probe. After penetration into the bacterial cells, Pep27 was not affected by macromolecular synthesis, but activated protein phosphatase activity in dose ranges of 10-15 microM and time range of 5-10 min in case of Staphylococcus epidermidis and Pseudomonas aeruginosa, respectively. These results demonstrated the antibacterial activities of Pep27 are fundamentally attributable to a physiological change by activated phosphatase activity.
Sung
Woo Sang
WS
Department of Microbiology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Puk-ku, Daegu 702-701, Korea.
Park
Yoonkyung
Y
Choi
Cheol-Hee
CH
Hahm
Kyung-Soo
KS
Lee
Dong Gun
DG
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000493:pharmacokinetics / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005434:Flow Cytometry; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000493:pharmacokinetics / Q000494:pharmacology*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D021382:Protein Sorting Signals; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D019810:Sodium Azide / Q000494:pharmacology; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010455:Peptides; D021382:Protein Sorting Signals; D019810:Sodium Azide; D010749:Phosphoprotein Phosphatases