TFIIS elongation factor and Mediator act in conjunction during transcription initiation in vivo.
The transcription initiation and elongation steps of protein-coding genes usually rely on unrelated protein complexes. However, the TFIIS elongation factor is implicated in both processes. We found that, in the absence of the Med31 Mediator subunit, yeast cells required the TFIIS polymerase II (Pol II)-binding domain but not its RNA cleavage stimulatory activity that is associated with its elongation function. We also found that the TFIIS Pol II-interacting domain was needed for the full recruitment of Pol II to several promoters in the absence of Med31. This work demonstrated that, in addition to its thoroughly characterized role in transcription elongation, TFIIS is implicated through its Pol II-binding domain in the formation or stabilization of the transcription initiation complex in vivo.
Guglielmi
Benjamin
B
Commissariat à l'Energie Atomique, Institut de Biologie et Technologies de Saclay, Bâtiment 144, Commissariat à l'Energie Atomique/Saclay, Gif-sur-Yvette Cedex F-91191, France.
Soutourina
Julie
J
Esnault
Cyril
C
Werner
Michel
M
D000426:Alcohol Dehydrogenase / Q000235:genetics / Q000378:metabolism; D003547:Cysteine Synthase; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D056892:Mediator Complex; D008958:Models, Molecular; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D016296:Mutagenesis; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D017384:Sequence Deletion; D014158:Transcription, Genetic; D035602:Transcriptional Elongation Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D056892:Mediator Complex; D009097:Multienzyme Complexes; C492627:SOH1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D035602:Transcriptional Elongation Factors; C018648:transcription factor S-II; C516801:ADH1 protein, S cerevisiae; D000426:Alcohol Dehydrogenase; D003547:Cysteine Synthase; C103381:MET17 protein, S cerevisiae