Flow cytometric measurement of the clearance rate in the blue mussel Mytilus edulis and the development of a new individual exposure system for aquatic immunotoxicological studies.
Animals in poor health condition are not relevant biological models. The current study focused on the use of the clearance rate of Mytilus edulis to assess the gross physiological condition of individuals maintained in stressful experimental conditions. This approach was developed in a new, highly controlled experimental exposure device designed to investigate individual responses in aquatic ecotoxicological studies. Both clearance rate values and immune parameters analysis indicated that the health condition of mussels kept in 50ml tubes for 24h or 48h was not altered compared to controls, while most parameters were depressed after 72h. Moreover, this study confirms the relevance of flow cytometric for the measurement of clearance rate compared to techniques utilizing microscopy. Current results prompted us to perform further 24h chemical exposure using this "in tubo" device.
Duchemin
Matthieu B
MB
Laboratoire des Sciences de l'Environnement Marin, UMR CNRS 6539, Université de Bretagne Occidentale, place Nicolas Copernic, 29280 Plouzane, France. matthieu.duchemin@univ-brest.fr
Wessel
Nathalie
N
Fournier
Michel
M
Auffret
Michel
M
D000818:Animals; D004305:Dose-Response Relationship, Drug; D054750:Ecotoxicology / Q000379:methods; D004781:Environmental Exposure; D004784:Environmental Monitoring / Q000379:methods*; D005434:Flow Cytometry; D008657:Metabolic Clearance Rate; D049880:Mytilus edulis / Q000187:drug effects / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics*
D014874:Water Pollutants, Chemical