A novel ovary-specific and ovulation-associated variant of epoxide hydrolase 2.
Ovulation is a complex process initiated by the surge of the pituitary luteinizing hormone (LH) that provokes the expression of specific genes. We report herein the isolation and characterization of an ovulation-associated, ovary-specific novel isoform of epoxide hydrolase 2 (Ephx2), Ephx2C. This variant is exclusively expressed in the granulosa cells of preovulatory mouse ovarian follicles. The LH-induced expression of Ephx2C is mediated by the protein kinase A and partially by the protein kinase C signaling pathways. The involvement of p38 kinase has also been demonstrated.
Shkolnik
Ketty
K
Department of Biological Regulation, Weizmann Institute of Science, Rehovot 76100, Israel.
Ben-Dor
Shifra
S
Galiani
Dalia
D
Hourvitz
Ariel
A
Dekel
Nava
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004851:Epoxide Hydrolases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010053:Ovary / Q000201:enzymology*; D010060:Ovulation; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D015398:Signal Transduction; D014018:Tissue Distribution; D015854:Up-Regulation
D020033:Protein Isoforms; D012333:RNA, Messenger; D004851:Epoxide Hydrolases; C518648:Ephx2 protein, mouse