Separation of corticosteroids by microemulsion EKC with diethyl L-tartrate as the oil phase.
A novel microemulsion based on a mixture of diethyl L-tartrate (DET) and SDS was developed for the microemulsion EKC (MEEKC) determination of structurally related steroids. The system consisted of 0.5% w/w DET, 1.7% w/w SDS, 1.2% w/w 1-butanol, 89.6% w/w phosphate buffer (40 mM, pH 7.0), and 7% w/w ACN. With an applied voltage of +10 kV, a baseline separation of aldosterone (A), cortisone acetate (CA), dexamethasone (D), hydrocortisone (H), hydrocortisone acetate (HA), prednisolone (P), prednisolone acetate (PA), prednisone (Ps), triamcinolone (T), and triamcinolone acetonide (TA) could be achieved. Under the optimized conditions, the reproducibility of the retention time (n = 4) for most of the compounds was less than +/-0.8% with the exception of A, Ps, and T. The average number of theoretical plates was 18 800 plates/m. The results were compared with those achieved by the modified micellar EKC (MEKC). MEEKC showed obvious advantages over MEKC for the separation of highly hydrophobic substances. To further evaluate the system, we tested the MEEKC method by analyzing corticosteroids in a spiked urine sample.
Wu
Chi-Hung
CH
Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Chen
Tse-Hsien
TH
Huang
Kuan-Pin
KP
Wang
Guan-Ren
GR
Liu
Chuen-Ying
CY
D000305:Adrenal Cortex Hormones / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D000450:Aldosterone / Q000302:isolation & purification / Q000652:urine; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003348:Cortisone / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000652:urine; D004655:Emulsions / Q000737:chemistry*; D006854:Hydrocortisone / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000652:urine; D057927:Hydrophobic and Hydrophilic Interactions; D011239:Prednisolone / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000652:urine; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013644:Tartrates / Q000737:chemistry*
D000305:Adrenal Cortex Hormones; D002021:Buffers; D004655:Emulsions; D013644:Tartrates; D012967:Sodium Dodecyl Sulfate; C021650:hydrocortisone acetate; D000450:Aldosterone; C009935:prednisolone acetate; D011239:Prednisolone; C064501:diethyl tartrate; D003348:Cortisone; D006854:Hydrocortisone