title: A rat in vitro model for the measurement of multiple excitability properties of cutaneous axons.
abstract: OBJECTIVE
  To establish an in vitro model for measurement of the excitability properties of cutaneous sensory axons.
  
  
  METHODS
  We used a saphenous skin-nerve preparation from adult rat in combination with computerized threshold tracking. We measured strength-duration time constant, the recovery of excitability after a supramaximal stimulus and the accommodation to conditioning subthreshold polarizing stimuli (threshold electrotonus, current-threshold relationship) and compared these with previously published recordings from sensory axons in human median nerve.
  
  
  RESULTS
  Threshold electrotonus and the amplitude of superexcitability were indistinguishable between human median nerve in vivo and rat saphenous nerve in vitro, but several excitability parameters were significantly different in the rat: strength-duration time constant was significantly shorter (0.19+/-0.01 vs. 0.53+/-0.02 ms); the refractory period was shorter (1.9+/-1.1 ms vs. 3.5+/-1.0 ms) and late subexcitability was smaller (6.3+/-0.3% vs. 11.3+/-0.5%); thirdly, during recording of current-threshold relationship, rat nerves displayed more inward rectification to strong hyperpolarizing currents. Parameters were stable over more than 3h.
  
  
  CONCLUSIONS
  Excitability changes of sensory Abeta-fibres can be reliably studied in the rat in vitro and are qualitatively similar to humans.
  
  
  CONCLUSIONS
  This rat model will facilitate pharmacological studies of nerve excitability and work on models of neuropathy.
authors: UCL Institute of Child Health, UCL, 30 Guilford St., London WC1N 1 EH, United Kingdom.
authors: Konrad
authors: K
authors: Maurer
authors: Hugh
authors: H
authors: Bostock
authors: Martin
authors: M
authors: Koltzenburg
mesh_terms: D000200:Action Potentials / Q000502:physiology* / Q000528:radiation effects; D000818:Animals; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation / Q000379:methods; D004576:Electromyography / Q000379:methods; D005260:Female; D066298:In Vitro Techniques; D023421:Models, Animal; D009431:Neural Conduction / Q000502:physiology* / Q000528:radiation effects; D009475:Neurons, Afferent / Q000502:physiology* / Q000528:radiation effects; D010543:Peroneal Nerve / Q000502:physiology* / Q000528:radiation effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time; D012032:Refractory Period, Electrophysiological / Q000502:physiology / Q000528:radiation effects; D012684:Sensory Thresholds / Q000502:physiology / Q000528:radiation effects; D012867:Skin / Q000294:innervation*