Cryopreservation of dormant herbaceous peony (Paeonia lactiflora pall.) shoot-tips by desiccation.
A simplified technique for the cryoprotection of dormant shoot-tips was developed for the germplasm conservation of herbaceous peony (Paeonia lactiflora Pall.). The highest post-thaw regrowth level was obtained from shoot-tips desiccated by air drying for 5 h. The post-thaw regrowth of P. lactiflora. var. 'Mikang' was the highest (74.9 percent) among the five genotypes tested. The regeneration level after cryopreservation was highest (66approximate74%; average of 70 percent) for dormant shoot-tips collected from November to February. Shoot tips which were dissected from non-dormant buds in late March, however, showed very low post-thaw regrowth. Post-thaw regrowth of shoot-tips was promoted in MS medium containing 1 mg 1(-1) of gibberellic acid (GA3) and 0.5 mg 1(-1) of N(6)-benzyladenine (BA). The elongated shoots were rooted on a 1/4 MS medium containing 0.1 mg 1(-1) of beta-naphthalene acetic acid (NAA). In addition, random amplified polymorphic DNAs (RAPDs) assays suggested that the cryostorage treatment used here preserved the genetic fidelity of the peony dormant shoot-tips. This cryopreservation method appears to be promising for the conservation of herbaceous peony germplasm.
Seo
Mi Jin
MJ
Department of Agronomy, Kyungpook National University, Daegu, Republic of Korea.
Shin
Jong Hee
JH
Sohn
Jae Keun
JK
D005612:Freeze Drying / Q000379:methods*; D029596:Paeonia / Q000235:genetics / Q000502:physiology; D018520:Plant Shoots / Q000502:physiology; D019105:Random Amplified Polymorphic DNA Technique; D012038:Regeneration / Q000235:genetics