Protection against lethal subcutaneous challenge of virulent Y. pestis strain 141 using an F1-V subunit vaccine.
In this study, we designed and engineered a two-component recombinant fusion protein antigen as a vaccine candidate against the possible biological threat of Yersinia pestis. The recombinant F1-V protein was formulated with Alhydrogel. A four-time injection with a dosage of 10, 20 and 50 microg/mouse in about two months was adopted for vaccination. Serum antibodies and subclass of T helper cells were measured and analyzed. After the final vaccination, the mice were challenged by 141 strain with 25-600 LD(50). In conclusion, the recombinant vaccine was capable of inducing protective immunity against subcutaneous challenge. The level of serum IgG was supposed to be a main factor that affected the final protection of challenge. 20 microg recombinant protein could induce an endpoint titre of serum IgG as high as 51200, which was enough to afford 100% protection against 400 LD(50) virulent 141 challenge. The antibody isotype analysis showed that the vaccine induced predominantly an IgG1 rather than IgG2a response. Flow cytometric analysis revealed that Alhydrogel significantly helped induce a stronger humoral immunity instead of CTL cellular response. These findings suggested that the plague F1-V subunit vaccine is promising for the next plague vaccine.
Wang
Dong
D
Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China.
Jia
Nuan
N
Li
Peng
P
Xing
Li
L
Wang
Xiliang
X
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood; D000942:Antigens, Bacterial / Q000008:administration & dosage; D001426:Bacterial Proteins / Q000008:administration & dosage; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010930:Plague / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D010931:Plague Vaccine / Q000008:administration & dosage*; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013601:T-Lymphocytes / Q000378:metabolism; D022223:Vaccines, Subunit / Q000008:administration & dosage; D015010:Yersinia pestis / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D010931:Plague Vaccine; D011993:Recombinant Fusion Proteins; D022223:Vaccines, Subunit