Anti-Ro and anti-La autoantibodies induce TNF-alpha production by human salivary gland cells: an in vitro study.
In this report, we demonstrate that both TNFR1 and the TNFR2 are expressed on the salivary gland cell line A-253 cell membrane. Furthermore, cell treatment with anti-Ro and anti-La autoantibodies from Sjögren IgG determined TNF-alpha production, clarifying which could be the inducer of the extrinsic pathway of apoptosis in salivary gland cells.
Sisto
M
M
Dipartimento di Anatomia Umana ed Istologia, Universitá di Bari, Italia.
Lisi
S
S
D'Amore
M
M
Mitolo
V
V
Scagliusi
P
P
D000974:Antibodies, Antinuclear / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000502:physiology; D001324:Autoantigens / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D045744:Cell Line, Tumor / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000096:biosynthesis* / Q000235:genetics; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000096:biosynthesis* / Q000235:genetics; D012261:Ribonucleoproteins / Q000276:immunology*; D012468:Salivary Gland Neoplasms / Q000473:pathology; D012859:Sjogren's Syndrome / Q000276:immunology; D013363:Submandibular Gland / Q000166:cytology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics; D000097585:SS-B Antigen; D000099207:SS-A Antigen
D000974:Antibodies, Antinuclear; D001324:Autoantigens; D007074:Immunoglobulin G; D047888:Receptors, Tumor Necrosis Factor, Type I; D047889:Receptors, Tumor Necrosis Factor, Type II; D012261:Ribonucleoproteins; D014409:Tumor Necrosis Factor-alpha; D000097585:SS-B Antigen; D000099207:SS-A Antigen; C035356:SS-A antibodies