title: Behaviour of bacterial division protein FtsZ under a monolayer with phospholipid domains.
abstract: Assembly of the tubulin-like protein FtsZ at or near the cytoplasmic membrane is one of the earliest steps in division of bacteria such as Escherichia coli. Exactly what constitutes the site at which FtsZ acts is less clear. To investigate the influence of the membrane phospholipids on FtsZ localization and assembly, we have elaborated with the Langmuir technique a two-lipid monolayer made of dilauryl-phosphatidylethanolamine (DLPE) and dipalmitoyl-phosphatidylglycerol (DPPG). This monolayer comprised stable condensed domains in an expanded continuous phase. In the presence of GTP, FtsZ assembly disrupts the condensed domains within 5 min. After several hours, with or without GTP, FtsZ assembled into large aggregates at the domain interface. We suggest that the GTP-induced polymerization of FtsZ is coupled to the association of FtsZ protofilaments with domain interfaces.
authors: Polymères, Biopolymères, Membranes, UMR 6522 CNRS, Université de Rouen, UFR des Sciences, 76821, Mont Saint Aignan Cedex, France.
authors: Céline
authors: C
authors: Lafontaine
authors: Jean-Marc
authors: JM
authors: Valleton
authors: Nicole
authors: N
authors: Orange
authors: Vic
authors: V
authors: Norris
authors: Eugenia
authors: E
authors: Mileykovskaya
authors: Stéphane
authors: S
authors: Alexandre
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D002455:Cell Division; D003598:Cytoskeletal Proteins / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry*; D017434:Protein Structure, Tertiary
chemical_list: D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; C007445:1,2-dilauroylphosphatidylethanolamine; C030345:1,2-dipalmitoylphosphatidylglycerol