Use of the TRP1 auxotrophic marker for gene disruption and phenotypic analysis in yeast: a note of warning.
The TRP1 marker has been commonly used for gene disruption experiments and subsequent phenotypic analysis. However, introduction of the TRP1 gene into a trp1 strain markedly affects growth under many conditions used for phenotypic profiling. Therefore, its use in the past should be revisited and utilization of this marker should be avoided in future analyses.
González
Asier
A
Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Barcelona, Spain.
Larroy
Carolina
C
Biosca
Josep A
JA
Ariño
Joaquín
J
D019747:Aldose-Ketose Isomerases / Q000235:genetics* / Q000502:physiology; D015415:Biomarkers; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000502:physiology
D015415:Biomarkers; D029701:Saccharomyces cerevisiae Proteins; D019747:Aldose-Ketose Isomerases; C490353:TRP1 protein, S cerevisiae