title: Purification and characterization of a novel alcohol oxidase from Paenibacillus sp. AIU 311.
abstract: An oxidase catalyzing the conversion of glycolaldehyde to glyoxal was purified to the homogeneous state from Paenibacillus sp. AIU 311, and its properties were revealed. This enzyme was specific to glycolaldehyde and glyceraldehyde, and the reaction rates to other alcohols and aldehydes were less than 6% of that of glycolaldehyde. The Km values for glycolaldehyde and glyceraldehyde were estimated to be 13.2 and 7.5 mM, respectively. The glycolaldehyde oxidation was optimum at pH 6.5 and 50 degrees C. The molecular mass of this enzyme was 49 kDa, and it consisted of two identical subunits of 24 kDa. The NH2-terminal sequence was not homologous to those of alcohol oxidases. This is the first report of an oxidase exhibiting high specificity to a hydroxy group of aldehyde alcohols.
authors: Department of Agro-bioscience, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan. kiso@iwate-u.ac.jp
authors: Kimiyasu
authors: K
authors: Isobe
authors: Ayako
authors: A
authors: Kato
authors: Yasutaka
authors: Y
authors: Sasaki
authors: Shuuya
authors: S
authors: Suzuki
authors: Michihiko
authors: M
authors: Kataoka
authors: Jun
authors: J
authors: Ogawa
authors: Akira
authors: A
authors: Iwasaki
authors: Junzo
authors: J
authors: Hasegawa
authors: Sakayu
authors: S
authors: Shimizu
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D001407:Bacillus / Q000145:classification* / Q000201:enzymology*; D004789:Enzyme Activation; D004795:Enzyme Stability; D013045:Species Specificity; D013379:Substrate Specificity
chemical_list: D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase