Combined estrogen and ghrelin administration restores number of blood vessels and collagen type I/III ratio in the urethral and anal canal submucosa of old ovariectomized rats.
We compare the effects of estrogen and/or ghrelin on vascular counts and collagen I/III ratio of urethral and anal canal submucosa in old vs young-adult ovariectomized rats. Ovariectomized Fisher 344 rats (18 and 3 months old, n = 24 x 2) received 42 daily intraperitoneal 17-ss estradiol (10 microg/kg), ghrelin (2 microg/kg), both, or vehicle (n = 6 x 4 per group). Blood vessel counts and collagen I/III ratio were measured, respectively, by light microscopy and Western blot analysis with immunohistochemistry of ghrelin receptors. Estrogen significantly increased urethral and anal vascular counts and collagen I/III ratio in young-adult rats. In old rats, only combined estrogen/ghrelin administration significantly increased both variables. This was not observed with estrogen or ghrelin separately. Ghrelin receptors were immunostained in urethral and anal submucosa of all samples. Combined estrogen/ghrelin administration restored postovariectomy urethral and anal canal submucosal vessel number and collagen I/III ratio in old rats suggesting independent ageing effect.
Rizk
Diaa E E
DE
Department of Obstetrics and Gynecology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates. rizk.diaa@uaeu.ac.ae
Hassan
Hazem A
HA
Al-Marzouqi
Ahmed H
AH
Ramadan
Gaber A
GA
Al-Kedrah
Soha S
SS
Daoud
Sayel A
SA
Fahim
Mohamed A
MA
D000367:Age Factors; D001003:Anal Canal / Q000098:blood supply* / Q000378:metabolism; D000818:Animals; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D004958:Estradiol / Q000008:administration & dosage* / Q000494:pharmacology; D005260:Female; D054439:Ghrelin / Q000008:administration & dosage* / Q000494:pharmacology; D009092:Mucous Membrane / Q000098:blood supply / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010052:Ovariectomy; D051381:Rats; D011916:Rats, Inbred F344; D014521:Urethra / Q000098:blood supply* / Q000378:metabolism
D024042:Collagen Type I; D024061:Collagen Type III; D054439:Ghrelin; D004958:Estradiol