title: A comparison of the production of reactive oxygen species by suspended particulate matter and diesel exhaust particles with macrophages.
abstract: Oxidative stress has emerged as a pivotal mechanism that underlies the toxic pulmonary effects of suspended particulate matter (SPM). Experimental evidence shows that redox-active transition metals, redox-cycling quinoids, and polycyclic aromatic hydrocarbons (PAHs) contained in SPM act synergistically, producing reactive oxygen species (ROS). The direct production of superoxide anion and the damaging hydroxyl radical has been studied in aqueous and dimethyl sulfoxide (DMSO) suspensions of SPM both with and without H2O2; however, no study has reported on the release of ROS from ingesting macrophages with SPM. We investigated the time course of the ability to induce lucigenin-dependent chemiluminescence (CL) from human monocyte-derived macrophages exposed to SPM, carbon black particles, and diesel exhaust particles (DEP). We also examined hydroxyl radical generation from the same experimental system using the 2-deoxy-d-robse method. We found an increase of CL for SPM, but not for carbon black particles or for DEP. Hydroxyl radical generation was observed in both SPM and DEP, but the release from DEP was more frequent than that from SPM. These results suggest that certain components of SPM are important in the response of ROS from ingesting macrophages with SPM, and that those components are discharged from SPM into the atmosphere.
authors: Osaka Prefectural Institute of Public Health, Osaka, Japan. ohyama@iph.pref.osaka.jp
authors: M
authors: M
authors: Ohyama
authors: T
authors: T
authors: Otake
authors: S
authors: S
authors: Adachi
authors: T
authors: T
authors: Kobayashi
authors: K
authors: K
authors: Morinaga
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D052638:Particulate Matter / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D001335:Vehicle Emissions
chemical_list: D052638:Particulate Matter; D017382:Reactive Oxygen Species; D001335:Vehicle Emissions