title: The heat-shock response of Listeria monocytogenes comprises genes involved in heat shock, cell division, cell wall synthesis, and the SOS response.
abstract: The food-borne pathogen Listeria monocytogenes has the ability to survive extreme environmental conditions due to an extensive interacting network of stress responses. It is able to grow and survive at relatively high temperatures in comparison with other non-sporulating food-borne pathogens. To investigate the heat-shock response of L. monocytogenes, whole-genome expression profiles of cells that were grown at 37 degrees C and exposed to 48 degrees C were examined using DNA microarrays. Transcription levels were measured over a 40 min period after exposure of the culture to 48 degrees C and compared with those of unexposed cultures at 37 degrees C. After 3 min, 25 % of all genes were differentially expressed, while after 40 min only 2 % of all genes showed differential expression, indicative of the transient nature of the heat-shock response. The global transcriptional response was validated by analysing the expression of a set of 13 genes by quantitative PCR. Genes previously identified as part of the class I and class III heat-shock response and the class II stress response showed induction at one or more of the time points investigated. This is believed to be the first study to report that several heat-shock-induced genes are part of the SOS response in L. monocytogenes. Furthermore, numerous differentially expressed genes that have roles in the cell division machinery or cell wall synthesis were down-regulated. This expression pattern is in line with the observation that heat shock results in cell elongation and prevention of cell division.
authors: Laboratory of Food Microbiology, Wageningen University and Research Centre, Bomenweg 2, 6703 HD Wageningen, The Netherlands.|Wageningen Centre for Food Sciences (WCFS), Diedenweg 20, 6703 GW Wageningen, The Netherlands.|Division of Health and Safety, NIZO Food Research, Kernhemseweg 2, 6718 ZB Ede, The Netherlands.
authors: Stijn
authors: S
authors: van der Veen
authors: Institute of Medical Microbiology, Justus-Liebig University, Frankfurter Strasse 107, 35392 Giessen, Germany.
authors: Torsten
authors: T
authors: Hain
authors: Division of Health and Safety, NIZO Food Research, Kernhemseweg 2, 6718 ZB Ede, The Netherlands.
authors: Jeroen A
authors: JA
authors: Wouters
authors: Institute of Medical Microbiology, Justus-Liebig University, Frankfurter Strasse 107, 35392 Giessen, Germany.
authors: Hamid
authors: H
authors: Hossain
authors: Wageningen Centre for Food Sciences (WCFS), Diedenweg 20, 6703 GW Wageningen, The Netherlands.
authors: Willem M
authors: WM
authors: de Vos
authors: Laboratory of Food Microbiology, Wageningen University and Research Centre, Bomenweg 2, 6703 HD Wageningen, The Netherlands.|Wageningen Centre for Food Sciences (WCFS), Diedenweg 20, 6703 GW Wageningen, The Netherlands.
authors: Tjakko
authors: T
authors: Abee
authors: Institute of Medical Microbiology, Justus-Liebig University, Frankfurter Strasse 107, 35392 Giessen, Germany.
authors: Trinad
authors: T
authors: Chakraborty
authors: Division of Health and Safety, NIZO Food Research, Kernhemseweg 2, 6718 ZB Ede, The Netherlands.|Wageningen Centre for Food Sciences (WCFS), Diedenweg 20, 6703 GW Wageningen, The Netherlands.
authors: Marjon H J
authors: MHJ
authors: Wells-Bennik
mesh_terms: D002455:Cell Division / Q000235:genetics; D002473:Cell Wall / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D018869:Heat-Shock Response / Q000235:genetics / Q000502:physiology*; D006358:Hot Temperature; D008089:Listeria monocytogenes / Q000235:genetics / Q000502:physiology*; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013014:SOS Response, Genetics
chemical_list: D012329:RNA, Bacterial; D012333:RNA, Messenger