title: Evaluation of cytomegalovirus (CMV) DNA quantification in dried blood spots: retrospective study of CMV congenital infection.
abstract: We compared two protocols for extracting DNA from dried blood spots for cytomegalovirus (CMV) DNA detection and quantification by real-time PCR. Both extraction methods were reliable for the retrospective diagnosis of CMV congenital infection. Quantification of CMV DNA was valuable after normalization of viral loads with albumin gene PCR amplification results.
authors: Service de Microbiologie Immunologie Biologique, Assistance Publique-Hôpitaux de Paris, Hôpital Antoine Béclère, Université Paris Sud 11, Clamart, France.
authors: Christelle
authors: C
authors: Vauloup-Fellous
authors: Aurélie
authors: A
authors: Ducroux
authors: Vincent
authors: V
authors: Couloigner
authors: Sandrine
authors: S
authors: Marlin
authors: Olivier
authors: O
authors: Picone
authors: Julie
authors: J
authors: Galimand
authors: Natalie
authors: N
authors: Loundon
authors: Françoise
authors: F
authors: Denoyelle
authors: Liliane
authors: L
authors: Grangeot-Keros
authors: Marianne
authors: M
authors: Leruez-Ville
mesh_terms: D002675:Child, Preschool; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000151:congenital* / Q000175:diagnosis*; D004279:DNA, Viral / Q000097:blood*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction / Q000379:methods*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D019562:Viral Load
chemical_list: D004279:DNA, Viral