[Influence of the delay in urinary NMP22 preanalytical processing].
OBJECTIVE
To evaluate the influence of the delay in urinary NMP22 preanalytical processing.


METHODS
Twenty-eight voided urine samples were taken: bladder cancer (14), urine tract infections (4), lithiasis (4), healthy volunteers (1), and with other no malignant bladder diseases (5). All samples, were maintained at environment temperature, and were processed according to the stabilization of parts of urine collected at 0, 30, 90 and 150 minutes. Samples were stored at 4 degrees C until its determination. NMP22 was determined with the IMMULITE One analyzer.


RESULTS
There were no significant differences for NMP22 levels between each different point of time studied.


CONCLUSIONS
Delay up to 2 hours and a half when we add stabilization solution to urine samples no affects NMP22 results. That thing, might provide more confidence and flexibility on quantitative immunoassays that required urine stabilization.
Fatela-Cantillo
D
D
Area de Biotecnología, Centro Hospitalario de Alta Resolución Sierra del Segura, EPHAG, La Puerta de Segura-Puente de Génave, Jaén. danielfatela@yahoo.es
Fernández-Suárez
A
A
Menéndez-López
V
V
Carro Rubias
C
C
Galán
J A
JA
García Serrado
D
D
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000652:urine*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000652:urine*; D013997:Time Factors
D014408:Biomarkers, Tumor; D009687:Nuclear Proteins; C100379:nuclear matrix protein 22