Nuclear-mitochondrial cross-talk during heat shock in Arabidopsis cell culture.
Apart from energy generation, mitochondria perform a signalling function determining the life and death of a cell under stress exposure. In the present study we have explored patterns of heat-induced synthesis of Hsp101, Hsp70, Hsp17.6 (class I), Hsp17.6 (class II) and Hsp60, and the development of induced thermotolerance in Arabidopsis thaliana cell culture under conditions of mitochondrial dysfunction. It was shown that treatment by mitochondrial inhibitors and uncouplers at the time of mild heat shock downregulates HSP synthesis, which is important for induced thermotolerance in plants. The exposure to elevated temperature induced an increase in cell oxygen consumption and hyperpolarization of the inner mitochondrial membrane. Taken together, these facts suggest that mitochondrial functions are essential for heat-induced HSP synthesis and development of induced thermotolerance in A. thaliana cell culture, suggesting that mitochondrial-nuclear cross-talk is activated under stress conditions. Treatment of Arabidopsis cell culture at 50 degrees C initiates a programmed cell death determined by the time course of viability decrease, DNA fragmentation and cytochrome c release from mitochondria. As treatment at 37 degrees C protected Arabidopsis cells from heat-induced cell death, it may be suggested that Hsp101, Hsp70 and small heat-shock proteins, the synthesis of which is induced under these conditions, are playing an anti-apoptotic role in the plant cell. On the other hand, drastic heat shock upregulated mitochondrial Hsp60 synthesis and induced its release from mitochondria to the cytosol, indicating a pro-apoptotic role of plant Hsp60.
Rikhvanov
Eugene G
EG
Siberian Institute of Plant Physiology and Biochemistry, Siberian Division, Russian Academy of Sciences, Lermontov St. 132, Irkutsk 664033, Russia. eugene@sifibr.irk.ru
Gamburg
Kim Z
KZ
Varakina
Nina N
NN
Rusaleva
Tatyana M
TM
Fedoseeva
Irina V
IV
Tauson
Elena L
EL
Stupnikova
Irina V
IV
Stepanov
Alexey V
AV
Borovskii
Genadii B
GB
Voinikov
Victor K
VK
D000968:Antimycin A / Q000031:analogs & derivatives / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D045304:Cytochromes c / Q000378:metabolism; D004136:Dinitrobenzenes / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000378:metabolism; D006358:Hot Temperature; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature
D029681:Arabidopsis Proteins; D004136:Dinitrobenzenes; D006360:Heat-Shock Proteins; C032456:antimycin; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000968:Antimycin A; D045304:Cytochromes c; C031536:dinitroaminophenol