EWS/FLI-1 induces rapid onset of myeloid/erythroid leukemia in mice.
EWS/FLI-1 is a chimeric oncogene generated by chromosomal translocation in Ewing tumors, a family of poorly differentiated pediatric tumors arising predominantly in bone but also in soft tissue. The fusion gene combines sequences encoding a strong transactivating domain from the EWS protein with the DNA binding domain of FLI-1, an ETS transcription factor. A related fusion, TLS/ERG, has been found in myeloid leukemia. To determine EWS/FLI-1 function in vivo, we engineered mice with Cre-inducible expression of EWS/FLI-1 from the ubiquitous Rosa26 locus. When crossed with Mx1-cre mice, Cre-mediated activation of EWS/FLI-1 resulted in the rapid development of myeloid/erythroid leukemia characterized by expansion of primitive mononuclear cells causing hepatomegaly, splenomegaly, severe anemia, and death. The disease could be transplanted serially into naïve recipients. Gene expression profiles of primary and transplanted animals were highly similar, suggesting that activation of EWS/FLI-1 was the primary event leading to disease in this model. The Cre-inducible EWS/FLI-1 mouse provides a novel model system to study the contribution of this oncogene to malignant disease in vivo.
Torchia
Enrique C
EC
Department of Developmental Neurobiology, Hartwell Center, St. Jude Children's Research Hospital, 332 N. Lauderdale St., Memphis, Tennessee 38105, USA.
Boyd
Kelli
K
Rehg
Jerold E
JE
Qu
Chunxu
C
Baker
Suzanne J
SJ
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D002678:Chimera; D002869:Chromosome Aberrations; D050982:GATA1 Transcription Factor / Q000378:metabolism; D020869:Gene Expression Profiling; D007951:Leukemia, Myeloid / Q000209:etiology / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008822:Mice, Transgenic; D009368:Neoplasm Transplantation; D015514:Oncogene Proteins, Fusion / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D051789:Proto-Oncogene Protein c-fli-1 / Q000235:genetics / Q000378:metabolism*; D022661:RNA, Untranslated; D034802:RNA-Binding Protein EWS; D012512:Sarcoma, Ewing; D013234:Stem Cells / Q000502:physiology
C106031:EWS-FLI fusion protein; D050982:GATA1 Transcription Factor; C494689:Gata1 protein, mouse; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D015514:Oncogene Proteins, Fusion; D011506:Proteins; D051789:Proto-Oncogene Protein c-fli-1; D022661:RNA, Untranslated; D034802:RNA-Binding Protein EWS