title: Confirmatory real-time PCR assay for human papillomavirus (HPV) type 52 infection in anogenital specimens screened for HPV infection with the linear array HPV genotyping test.
abstract: A novel real-time PCR assay for detection of human papillomavirus type 52 (HPV-52) DNA (RT-52) was evaluated on 265 anogenital samples. RT-52 had a sensitivity of 98.4% and a specificity of 100% compared to conventional HPV-52 typing assays, including hybridization of PGMY products with an HPV-52-specific probe and PCR sequencing of HPV-52 E6.
authors: Département de Microbiologie et Infectiologie, Hôpital Notre-Dame du Centre Hospitalier de l'Université de Montréal, Montréal, Québec, Canada. francois.coutlee@ssss.gouv.qc.ca
authors: François
authors: F
authors: Coutlée
authors: Danielle
authors: D
authors: Rouleau
authors: Georges
authors: G
authors: Ghattas
authors: Catherine
authors: C
authors: Hankins
authors: Sylvie
authors: S
authors: Vézina
authors: Pierre
authors: P
authors: Coté
authors: John
authors: J
authors: Macleod
authors: Alexandra
authors: A
authors: de Pokomandy
authors: Deborah
authors: D
authors: Money
authors: Sharon
authors: S
authors: Walmsley
authors: Hélène
authors: H
authors: Voyer
authors: Paul
authors: P
authors: Brassard
authors: Eduardo
authors: E
authors: Franco
mesh_terms: D001003:Anal Canal / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005835:Genitalia / Q000821:virology*; D005838:Genotype; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral