title: Pathogenicity, antibiotic susceptibility and genetic similarity of environmental and clinical isolates of Vibrio cholerae.
abstract: Isolates of Vibrio cholerae were obtained from clinical and environmental samples and the pathogenicity of these isolates was confirmed by hemolytic assay. The clinical isolates were more pathogenic than environmental isolates. Antibiotic susceptibility of V. cholerae to a set of antibiotics showed a marked variation. The environmental isolates exhibited more resistance to the antibiotics than clinical isolates. The plasmid curing technique was used to check the encoding of antibiotic resistance gene in genome. In both isolates, the resistance to vancomycin and co-trimaxazole was not mediated by plasmid and it may probably be encoded in genome. RAPD method was adopted to find out the variation in the genome of the clinical isolates and environmental isolates of V. cholerae. The genomic similarity pattern revealed that the environmental Ogawa isolates were closely related to clinical Ogawa isolates. This study confirmed the existence of the complex nature of V. cholerae in its pathogenicity, response to a set of antibiotics and genetic similarity.
authors: P G Department of Immunology and Microbiology, The American College, Madurai 625 002, India.
authors: D Immanual Gilwax
authors: DI
authors: Prabhu
authors: R Selvaraj
authors: RS
authors: Pandian
authors: P Thirumalai
authors: PT
authors: Vasan
mesh_terms: D000818:Animals; D001483:Base Sequence; D002771:Cholera / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004783:Environmental Microbiology; D014644:Genetic Variation; D006461:Hemolysis; D006801:Humans; D019105:Random Amplified Polymorphic DNA Technique; D014734:Vibrio cholerae / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D004269:DNA, Bacterial