A new pyrrole alkaloid isolated from Arum palaestinum Boiss. and its biological activities.
The phytochemical analysis of the ethyl acetate fraction of Arum palaestinum Boiss. (Araceae) led to the isolation and identification of a new polyhydroxy alkaloid compound; (S)-3,4,5-trihydroxy-1 H-pyrrol-2(5H)-one (1), and other five known compounds; caffeic acid (2), isoorientin (3), luteolin (4) and vicenin 11 (5), as well as the rare compound 3,6,8-trimethoxy, 5,7,3',4'-tetrahydroxy flavone (6). The structural elucidations of all the compounds were based on spectroscopic data (1H- and 13C-NMR, DEPT, HSQC, HMBC and NOE difference techniques) and comparison with literature data. Investigation of the antioxidant activity of the ethyl acetate fraction indicated its strong scavenging capacity for 1,1 -diphenyl-2-picrylhydrazyl (DPPH) radicals (SC50 3.1+/-0.82 microg/mL). Moreover, the treatment of different human cancer cell lines with the ethyl acetate fraction led to dose-dependant suppression in the proliferation of both breast carcinoma cells (MCF-7; IC50 59.09+/-4.1 microg/mL) and lymphoblastic leukemia cells (1301; IC50 53.1+/-2.9 microg/mL); however, it was found to have no effect on the growth of hepatocellular carcinoma cells (Hep G2).
El-Desouky
S K
SK
College of Forest Science, Kookmin University, Seoul, Korea.
Kim
Ki Hun
KH
Ryu
Shi Young
SY
Eweas
Ahmad Farouk
AF
Gamal-Eldeen
Amira M
AM
Kim
Young-Kyoon
YK
D000085:Acetates / Q000737:chemistry; D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology; D031058:Arum / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D011758:Pyrroles / Q000302:isolation & purification / Q000494:pharmacology
D000085:Acetates; D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D016166:Free Radical Scavengers; D010936:Plant Extracts; D011758:Pyrroles; C007650:ethyl acetate