title: [Adriamycin combined with hyperthermia inhibits transplanted B cell lymphoma in nude mice].
abstract: OBJECTIVE
  To study the effect of adriamycin combined with hyperthermia on tumor formation and growth of human B lymphoma cell line (Raji) in nude mice.
  
  
  METHODS
  Twenty-four BALB/C nude mice were divided into control group (37 degrees celsius;), chemotherapy group (37 degrees celsius;+ADM), hyperthermia group (42 degrees celsius;) and thermochemotherapy group (42 degrees celsius;+ADM), and in each mouse, 5 x 10(6) Raji cells were injected subcutaneously. The time and rate of tumor formation were observed, the tumor diameter was measured every 3 days and the tumor volume calculated to obtain the growth curves of the tumors. Three weeks after tumor formation, all the mice were executed to observe the histopathological changes of the tumor with HE staining.
  
  
  RESULTS
  The time of tumor formation in the control, chemotherapy, hyperthermia and thermochemotherapy groups were 11.2-/+1.7, 20.2-/+2.3, 15.3-/+1.6 and 23.8-/+1.7 days, respectively. Three weeks after tumor formation, the average weight of the tumors were 3.33-/+0.57, 2.26-/+0.28, 2.76-/+0. 26 and 1.73-/+0. 33 g, respectively, and the tumor growth inhibition rate was 48.0% in the thermochemotherapy group.
  
  
  CONCLUSIONS
  Thermochemotherapy can be effective in inhibiting tumor growth and provides an alternative of adjuvant therapy for malignant B cell lymphoma.
authors: Department of Oncology, 88 Hospital of PLA, Tai'an 271000, China. whmppx@163.com
authors: Hong-mei
authors: HM
authors: Wei
authors: Yi-min
authors: YM
authors: Ruan
authors: Li-cheng
authors: LC
authors: Zhang
authors: Yong
authors: Y
authors: Peng
authors: Kun-yuan
authors: KY
authors: Guo
mesh_terms: D000818:Animals; D018951:Antigens, CD20 / Q000378:metabolism; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D003131:Combined Modality Therapy; D004317:Doxorubicin / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D006979:Hyperthermia, Induced; D017493:Leukocyte Common Antigens / Q000378:metabolism; D016393:Lymphoma, B-Cell / Q000188:drug therapy / Q000235:genetics / Q000473:pathology* / Q000628:therapy; D008297:Male; D051379:Mice; D008819:Mice, Nude; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D018951:Antigens, CD20; D019253:Proto-Oncogene Proteins c-bcl-2; D004317:Doxorubicin; D017493:Leukocyte Common Antigens