title: Antibiotic susceptibility testing of Bifidobacterium thermophilum and Bifidobacterium pseudolongum strains: broth microdilution vs. agar disc diffusion assay.
abstract: There is urgent need for having available suitable methods and data regarding the susceptibility levels of antibiotic resistant and sensitive strains of bifidobacteria. Based on a defined standard operation procedure, agar disc diffusion and broth microdilution were compared in order to evaluate the antimicrobial susceptibility profiles of 82 B. pseudolongum and 80 B. thermophilum strains mainly originating from the meat production chain. The methods that were assessed showed interpretable agreement within this study. The disc diffusion zone diameters are highly reproducible making the method a useful alternative to broth microdilution for antimicrobial susceptibility screening of bifidobacteria.
authors: BOKU-University of Natural Resources and Applied Life Sciences, Department of Food Science and Technology, Division of Food Microbiology and Hygiene, Gregor Mendel Str. 33, A-1180 Vienna, Austria. konrad.domig@boku.ac.at
authors: Konrad J
authors: KJ
authors: Domig
authors: Sigrid
authors: S
authors: Mayrhofer
authors: Ulrike
authors: U
authors: Zitz
authors: Christiane
authors: C
authors: Mair
authors: Agnes
authors: A
authors: Petersson
authors: Ernst
authors: E
authors: Amtmann
authors: Helmut K
authors: HK
authors: Mayer
authors: Wolfgang
authors: W
authors: Kneifel
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001644:Bifidobacterium / Q000187:drug effects* / Q000254:growth & development; D015169:Colony Count, Microbial / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial; D005516:Food Microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D019936:Probiotics
chemical_list: D000900:Anti-Bacterial Agents