title: Alpha-tocopherol supplementation prevents the exercise-induced reduction of serum paraoxonase 1/arylesterase activities in healthy individuals.
abstract: OBJECTIVE
  To investigate PON 1/Aryl activities in basketball players with or without alpha-T supplementation pre- and post-training. Vitamin E (alpha-tocopherol, alpha-T) reduces lipid peroxidation. Paraoxonase 1/arylesterase (PON 1/Aryl) activities are closely related to oxidation and atherogenesis.
  
  
  METHODS
  Blood was obtained from 10 players pre- (group A), post-exercise (group B) and after 1 month on alpha-T (200 mg per 24 h orally) supplementation pre- (group C) and post-exercise (group D). Lactate, pyruvate, muscle enzyme activities, creatine kinase, lactate dehydrogenase and total antioxidant status (TAS) were measured with commercial kits. Catecholamines and alpha-T were determined with high-performance liquid chromatography methods and PON 1/Aryl activities spectrophotometrically.
  
  
  RESULTS
  Lactate, pyruvate, muscle enzyme activities and catecholamines were increased (P<0.001) in all groups post-training. Alpha-T levels remained unaltered pre- vs post-exercise. TAS was decreased in all the groups post training. PON 1/Aryl activities were significantly decreased post-exercise (group B) (PON1: 65+/-12 U min(-1) ml(-1), Aryl: 58+/-14 KU min(-1) ml(-1)) as compared to those pre-exercise (group A) (PON1: 142+/-16 U min(-1) ml(-1), Aryl: 114+/-12 KU min(-1) ml(-1), P<0.001). In contrast, the studied enzyme activities remained practically unaltered after alpha-T supplementation pre- vs post-training. Both enzyme activities positively correlated to TAS (r=0.60, P<0.001).
  
  
  CONCLUSIONS
  Alpha-T supplementation may result in protection of the enzyme PON 1/Aryl activities from free radical production.
authors: Department of Experimental Physiology, Medical School, University of Athens, Athens, Greece. stsakir@cc.uoa.gr
authors: S
authors: S
authors: Tsakiris
authors: G A
authors: GA
authors: Karikas
authors: T
authors: T
authors: Parthimos
authors: T
authors: T
authors: Tsakiris
authors: C
authors: C
authors: Bakogiannis
authors: K H
authors: KH
authors: Schulpis
mesh_terms: D000293:Adolescent; D000975:Antioxidants / Q000494:pharmacology*; D043303:Aryldialkylphosphatase / Q000037:antagonists & inhibitors* / Q000097:blood; D001490:Basketball / Q000502:physiology; D001794:Blood Pressure; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors* / Q000097:blood; D002395:Catecholamines / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D019587:Dietary Supplements; D015444:Exercise / Q000502:physiology*; D006339:Heart Rate / Q000187:drug effects; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects*; D008055:Lipids / Q000097:blood; D008297:Male; D009124:Muscle Proteins / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects; D019289:Pyruvic Acid / Q000097:blood; D055815:Young Adult; D024502:alpha-Tocopherol / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D002395:Catecholamines; D008055:Lipids; D009124:Muscle Proteins; D019344:Lactic Acid; D019289:Pyruvic Acid; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase; D024502:alpha-Tocopherol