title: Antimicrobial effect of triclosan and triclosan with Gantrez on five common endodontic pathogens.
abstract: Microbial control of the root canal system is one of the key objectives of root canal therapy. Triclosan is a widely accepted broad spectrum antimicrobial agent proven to be effective against many gram-positive and gram-negative bacteria. Triclosan acts by blocking bacterial fatty acid biosynthesis. The addition of Gantrez copolymer has been shown to enhance the antimicrobial activity of triclosan. The purpose of this study was to determine the minimum inhibitory and bactericidal concentrations of triclosan and triclosan with Gantrez against Prevotella intermedia, Fusobacterium nucleatum, Actinomyces naeslundii, Porphyromonas gingivalis, and Enterococcus faecalis. The minimum inhibitory concentration (MIC) of both test solutions was determined for each of the 5 microorganisms by using microtiter serial dilutions. Samples were streaked on 5% sheep blood agar plates and placed in an anaerobic incubator to determine the minimum bactericidal concentration (MBC). The MBC of triclosan ranged from 12-94 microg/mL. The MBC of triclosan with Gantrez ranged from <0.3-10.4 microg/mL. The addition of Gantrez enhanced the bactericidal activity of triclosan. Both triclosan and triclosan with Gantrez demonstrated bactericidal activity against the 5 specific endodontic pathogens.
authors: Private practice, Bloomingdale, Illinois, USA.
authors: William J
authors: WJ
authors: Nudera
authors: Mohamed I
authors: MI
authors: Fayad
authors: Bradford R
authors: BR
authors: Johnson
authors: Min
authors: M
authors: Zhu
authors: Christopher S
authors: CS
authors: Wenckus
authors: Ellen A
authors: EA
authors: Begole
authors: Christine D
authors: CD
authors: Wu
mesh_terms: D000190:Actinomyces / Q000187:drug effects; D000269:Adhesives / Q000494:pharmacology*; D000693:Anaerobiosis; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004357:Drug Synergism; D013293:Enterococcus faecalis / Q000187:drug effects; D016967:Fusobacterium nucleatum / Q000187:drug effects; D006089:Gram-Negative Anaerobic Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006801:Humans; D008298:Maleates / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011145:Polyvinyls / Q000494:pharmacology*; D016966:Porphyromonas gingivalis / Q000187:drug effects; D018713:Prevotella intermedia / Q000187:drug effects; D012388:Root Canal Irrigants / Q000494:pharmacology; D014260:Triclosan / Q000494:pharmacology*
chemical_list: D000269:Adhesives; D000891:Anti-Infective Agents, Local; D008298:Maleates; D011145:Polyvinyls; D012388:Root Canal Irrigants; C058026:polyvinylmethoxyethylene-maleic anhydride copolymer; D014260:Triclosan