[Evaluation of allelic alterations in 13 CODIS STR loci in tumor tissues from human digestive system].
OBJECTIVE
To study genetic alterations in 13 CODIS STR loci in various tumor tissue samples from human digestive system.


METHODS
Malignant tumor tissues and blood samples taken from 55 different unrelated individuals were collected. DNA samples were extracted using Chelex100 extraction kit, amplified using Profiler and Cofiler PCR amplification kit and analyzed using API 310 analyzer.


RESULTS
Aberrant cell divisions were detected in all of the 55 tumor tissue samples, with STR alternations detected in two samples including allelic alteration, partial and complete loss or unbalance of heterozygosity. Moreover, the alternations might occur simultaneously at more than one loci.


CONCLUSIONS
Caution must be taken in STR analysis of tumor tissue samples since the exclusion loci in forensic identification or paternity testing may be resulted from mutations in the tumor tissue.
Fang
Jian-xin
JX
Institute of Forensic Sciences, Ministry of Justice, PR China, Shanghai 200063, China. fangjx@ssfjd.cn
Li
Cheng-tao
CT
Xiao
Li
L
D000483:Alleles; D004273:DNA, Neoplasm / Q000032:analysis; D004067:Digestive System Neoplasms / Q000235:genetics*; D005770:Gastrointestinal Neoplasms / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D019656:Loss of Heterozygosity; D010190:Pancreatic Neoplasms / Q000235:genetics; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences / Q000235:genetics*
D004273:DNA, Neoplasm