Colonization of broilers by Campylobacter jejuni internalized within Acanthamoeba castellanii.
Although Campylobacter survives within amoeba in-vitro, it is unknown if intra-amoeba Campylobacter jejuni can colonize broilers. Five groups of 28 day-of-hatch chicks were placed into separate isolators. Groups (1) and (2) were challenged with page's amoeba saline (PAS), and disinfected planktonic C. jejuni NCTC 11168, respectively. Groups (3), (4) and (5) were challenged with a C. jejuni positive control, C. jejuni in PAS, and intra-amoeba C. jejuni, respectively. After 1, 3, 7 and 14 days post challenge, seven birds from each unit were examined for C. jejuni colonization. For the first time we report that intra-amoeba C. jejuni colonized broilers.
Snelling
William J
WJ
School of Biomedical Sciences, University of Ulster, Coleraine, BT52 1SA, County Londonderry, Northern Ireland, UK, b.snelling@ulster.ac.uk.
Stern
Norman J
NJ
Lowery
Colm J
CJ
Moore
John E
JE
Gibbons
Emma
E
Baker
Ciara
C
Dooley
James S G
JS
D048248:Acanthamoeba castellanii / Q000382:microbiology*; D000818:Animals; D002169:Campylobacter Infections / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000254:growth & development*; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D015169:Colony Count, Microbial; D011201:Poultry Diseases / Q000382:microbiology*