Sortagging: a versatile method for protein labeling.
Genetically encoded reporter constructs that yield fluorescently labeled fusion proteins are a powerful tool for observing cell biological phenomena, but they have limitations. Sortagging (sortase-mediated transpeptidation) is a versatile chemoenzymatic system for site-specific labeling of proteins with small (<2 kDa) probes. Sortagging combines the precision of a genetically encoded tag with the specificity of an enzymatic reaction and the ease and chemical versatility of peptide synthesis. Here we apply this technique to proteins in vitro and on the surface of living cells.
Popp
Maximilian W
MW
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA.
Antos
John M
JM
Grotenbreg
Gijsbert M
GM
Spooner
Eric
E
Ploegh
Hidde L
HL
D015374:Biosensing Techniques / Q000379:methods*; D002460:Cell Line; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D015335:Molecular Probes / Q000737:chemistry*; D015394:Molecular Structure
D008565:Membrane Proteins; D015335:Molecular Probes