Multiplex assay based on single-nucleotide polymorphisms for rapid identification of Brucella isolates at the species level.
The genus Brucella includes a number of species that are major animal pathogens worldwide and significant causes of zoonotic infections of humans. Traditional methods of identifying Brucella to the species level can be time-consuming, can be subjective, and can pose a hazard to laboratory personnel in the absence of suitable biocontainment facilities. Using a robust phylogenetic framework, a number of single-nucleotide polymorphisms (SNPs) that define particular species within the genus were identified. These SNPs were used to develop a multiplex SNP detection assay, based on primer extension technology, that can rapidly and unambiguously identify an isolate as a member of one of the six classical Brucella species or as a member of the recently identified marine mammal group.
Scott
Julie C
JC
Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, United Kingdom.
Koylass
Mark S
MS
Stubberfield
Michael R
MR
Whatmore
Adrian M
AM
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002002:Brucella / Q000145:classification / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D001426:Bacterial Proteins; D004269:DNA, Bacterial