Effect of storage xyloglucans on peritoneal macrophages.
Xyloglucans from seeds of Copaifera langsdorffii (XGC), Hymenaea courbaril (XGJ) and Mucuna sloanei (XGM) were obtained from milled and defatted cotyledons by aqueous extraction at 25 degrees C. The resulting fractions contained Glc, Xyl and Gal in molar ratios of 2.5: 1.5: 1.0 (XGC), 3.8: 2.6: 1.0 (XGJ) and 2.5: 1.6: 1.0 (XGM). HPSEC-MALLS/RI analysis showed that each polysaccharide fraction was homogeneous; M(w) values were 1.6 x 10(5), 2.0 x 10(5) and 1.5 x 10(5)g/mol, respectively. The effect of the xyloglucans on the production of O(2)*(-) and NO* and on the recruitment of macrophages to the mouse peritoneum was evaluated. All polysaccharides promoted an increase in the number of peritoneal macrophages in a dose-dependent manner. The largest increase, of 576% in comparison to the control group, was elicited by XGJ at 200 mg/kg. The effect of XGC, XGJ and XGM on O(2)*(-) production, in the presence or absence of phorbol 12-myristate 13-acetate (PMA), was not statistically significant. For NO(.) production, the lowest concentration of XGC (10 microg/ml) gave rise to an increase of 262% when compared to the control group; the effect was dose-dependent, reaching 307% at 50 microg/ml. On the other hand, XGJ at a concentration of 50 microg/ml enhanced NO* production by 92%. XGM did not affect NO* production significantly. The results indicate that xyloglucans from C. langsdorffii, H. courbaril and M. sloanei have immunomodulatory activity.
Rosário
M M T
MM
Universidade Federal do Paraná, Departamento de Bioquímica e Biologia Molecular, CP 19046, CEP 81531-990, Curitiba-PR, Brazil.
Noleto
G R
GR
Bento
J F
JF
Reicher
F
F
Oliveira
M B M
MB
Petkowicz
C L O
CL
D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel; D005936:Glucans / Q000737:chemistry / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009005:Monosaccharides / Q000737:chemistry; D009585:Nitrogen Dioxide / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014990:Xylans / Q000737:chemistry / Q000494:pharmacology*
D005936:Glucans; D009005:Monosaccharides; D014990:Xylans; D013481:Superoxides; C029353:xyloglucan; D009585:Nitrogen Dioxide