Long-lasting second stage of recognition memory consolidation in mice.
We investigated the effects of anisomycin (ANI) treatment (150 mg/kg s.c.) during late stages of memory consolidation in a juvenile recognition task in mice. ANI treatment blocked 24 h recognition memory if administered 9 h, 12 h or 15 h after learning. As shown by a significantly reduced incorporation of radioactively labelled amino acids into newly synthesized proteins, translational arrest by ANI treatment lasted for 3-4 h, thus covering the complete time period between 9 h and 18 h after memory acquisition. Together with previous findings [Richter K, Wolf G, Engelmann M. Social recognition memory requires two stages of protein synthesis in mice. Learn Mem 2005;12(4):407-13], our data suggest two distinct stages of protein synthesis to occur during the first 24 h after learning: an early, relatively short-stage, starting immediately after learning and lasting for approximately 3h, and a second stage starting 6 h after learning lasting for approximately 12 h. This is the first report of such a long-lasting protein synthesis-dependent second consolidation phase in mice and suggests that long-term consolidation of juvenile recognition memory comprises multiple waves of protein synthesis and complex cascades of inter- and intra-cellular signaling processes.
Wanisch
Klaus
K
Max-Planck-Institut für Psychiatrie, AG Neuronale Plastizität, Kraepelinstr. 2, D-80804 Munich, Germany.
Wotjak
Carsten T
CT
Engelmann
Mario
M
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D001522:Behavior, Animal; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004192:Discrimination, Psychological / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009833:Olfactory Pathways / Q000502:physiology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D021641:Recognition, Psychology / Q000187:drug effects / Q000502:physiology*; D012903:Smell; D013997:Time Factors
D000596:Amino Acids; D011500:Protein Synthesis Inhibitors; D000841:Anisomycin