Ultrastructural study on the specific binding of genetically engineered epidermal growth factor to type I collagen fibrils.
In an attempt to develop collagen-growth factor composites for use in tissue engineering, chimeric proteins consisting of epidermal growth factor and collagen binding domains derived from von Willebrand factor or fibronectin were synthesized by means of recombinant technology. These chimeric proteins were bound to type I collagen fibrils, and the ultrastructures of composites were analyzed by transmission electron microscopy combined with the gold nanoparticle labeling technique. The results of the ultrastructural study revealed that chimeric proteins were densely assembled on collagen fibrils through the specific recognition of binding sites, producing the ordered array of chimeric proteins.
Kato
Koichi
K
Institute for Frontier Medical Sciences, Kyoto University, Shogoin, Kyoto, Japan.
Sato
Hideki
H
Iwata
Hiroo
H
D001665:Binding Sites; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure*; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D046529:Microscopy, Electron, Transmission
D024042:Collagen Type I; D004815:Epidermal Growth Factor