Intrinsic novobiocin resistance in Staphylococcus saprophyticus.
Intrinsic novobiocin resistance in Staphylococcus saprophyticus was associated with expression of a novobiocin-resistant form of the drug target protein (GyrB). Site-directed mutagenesis established that resistance depends upon the presence of two specific amino acid residues in GyrB: a glycine at position 85 and a lysine at position 140.
Vickers
Anna A
AA
Antimicrobial Research Centre and Research Institute of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom.
Chopra
Ian
I
O'Neill
Alex J
AJ
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D027081:DNA Gyrase / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009675:Novobiocin / Q000494:pharmacology*; D017386:Sequence Homology, Amino Acid; D013210:Staphylococcus / Q000187:drug effects* / Q000235:genetics*
D000900:Anti-Bacterial Agents; D009675:Novobiocin; D027081:DNA Gyrase