Monoclonal antibodies to Mycobacterium tuberculosis CDC 1551 reveal subcellular localization of MPT51.
Mycobacterium tuberculosis CDC 1551, a highly immunogenic outbreak strain, previously reported to have unique surface distribution of capsular polysaccharide, was used to generate novel monoclonal antibodies (mabs) to surface mycobacterial targets. Two immunoglobulin G1 (IgG1) mAbs, 16a1 and 16a6 were generated. The mAbs originated from the same B cell, bound strongly to whole cell M. tuberculosis CDC1551 and to its cell wall, membrane and cytosol fractions recognizing a 90kDa protein. Immunoprecipitation using mAb 16a1 isolated a protein with amino acid peptide sequences matching MPT51 from the cytosol. This immunogenic protein of unknown function was previously reported only in culture filtrates of M. tuberculosis. Our findings suggest for the first time that this protein is found within the M. tuberculosis cell.
Al-Sayyed
Ban
B
Division of Pediatric Infectious Diseases, Department of Pediatrics, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Piperdi
Sajida
S
Yuan
Xinni
X
Li
Anping
A
Besra
Gurdyal S
GS
Jacobs
William R
WR
Casadevall
Arturo
A
Glatman-Freedman
Aharona
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D001422:Bacterial Adhesion / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D003600:Cytosol / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016253:Microscopy, Immunoelectron; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C082911:MPT51 protein, Mycobacterium