Commercial ampholytes used for isoelectric focusing may interfere with bioactivity based purification of antimicrobial peptides.
BioRad's Rotofor system has been frequently used for the purification of proteins and smaller peptides such as bacteriocins. In this study, we report that some commercially available ampholytes used with the Rotofor isoelectric focusing system possess antimicrobial activity, which may interfere with the purification of bacteriocins and bacteriocin-like substances.
Riazi
Shadi
S
Department of Food Science, Rutgers, the State University of New Jersey, New Brunswick, New Jersey 08901, USA.
Dover
Sara
S
Turovskiy
Yevgeniy
Y
Chikindas
Michael L
ML
D000665:Ampholyte Mixtures / Q000737:chemistry; D023181:Antimicrobial Cationic Peptides / Q000302:isolation & purification* / Q000378:metabolism; D001709:Biotechnology / Q000379:methods* / Q000592:standards; D007525:Isoelectric Focusing / Q000379:methods*
D000665:Ampholyte Mixtures; D023181:Antimicrobial Cationic Peptides