Characterization of CTX-M-22 and TEM-141 Encoded by a single plasmid from a clinical isolate of Enterobacter cloacae in China.
We analyzed the resistance to expanded-spectrum cephalosporins of an Enterobacter cloacae clinical isolate, EC002, by transconjugation, isoelectric-focusing analysis, and cloning experiments. It produced two beta-lactamases with isoelectric point values of 5.4 and 8.7, corresponding to TEM-141, a novel variant of TEM-1, and CTX-M-22, encoded by a transferable plasmid.
Liu
Gang
G
Pharmaceutical Research Institute and Department of Pharmacology, North Sichuan Medical College, Sichuan, China.
Ling
Bao-Dong
BD
Xie
Yong-En
YE
Lin
Li
L
Zeng
Yun
Y
Zhang
Xiang
X
Lei
Jun
J
D001483:Base Sequence; D002511:Cephalosporins / Q000494:pharmacology; D002681:China; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016972:Enterobacter cloacae / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000382:microbiology*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
D002511:Cephalosporins; D001618:beta-Lactamases