title: Bombesin promotes vasculogenesis and angiogenesis in chick chorio-allantoic membrane: A morphometric, structural, and ultrastructural study.
abstract: Experiments were performed on the chorio-allantoic membrane (CAM) of the chick to evaluate the effects of bombesin (BN) on vascular neoformation. In morphometrical assays, 10(-13)-10(-4) M BN promoted dose-dependent vascular development. Newly formed vessels converged toward the BN release site in a spoked wheel arrangement, suggesting a diffusion gradient mechanism. Structural and ultrastructural analysis of CAM specimens collected near the BN release site showed that both vasculogenetic and angiogenetic processes cooperated in vascular neoformation that involved committed cells from the mesenchyme (angioblasts) as well as endothelial cells. No pattern of vascular development was detected away from the BN release site. Findings from the present study emphasize the role of BN in vascular net development of respiratory organs.
authors: Department of Animal Biology and Genetics, University of Florence, I-50125 Florence, Italy.
authors: Sara
authors: S
authors: Quagliata
authors: Stefania
authors: S
authors: Pacini
authors: Tiziana
authors: T
authors: Punzi
authors: Cecilia
authors: C
authors: Malentacchi
authors: Marco
authors: M
authors: Ruggiero
authors: Giovanni
authors: G
authors: Delfino
mesh_terms: D000482:Allantois / Q000166:cytology / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D000818:Animals; D001839:Bombesin / Q000494:pharmacology*; D002642:Chick Embryo; D046529:Microscopy, Electron, Transmission; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D018377:Neurotransmitter Agents / Q000494:pharmacology
chemical_list: D018377:Neurotransmitter Agents; D001839:Bombesin