title: Jones oxidation and high performance liquid chromatographic analysis of cholesterol in biological samples.
abstract: A simple pre-column derivatization procedure for HPLC analysis of cholesterol in biological samples was developed. Cholesterol was treated with chromic acid and sulfuric acid in acetone (the Jones oxidation) and cholest-4-en-3,6-dione was formed. The reaction was finished in 5 min at room temperature and the product showed a strong UV absorbance at 250 nm that enabled an HPLC detection limit of 0.2 pmol. With stigmasterol as an internal standard, the reaction was applied to the analysis of total and free cholesterol in serum and high-density lipoproteins and the analysis showed a within-run and total coefficient of variation of about 0.2% and 0.5%, respectively.
authors: Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100710, China.
authors: Jun
authors: J
authors: Dong
authors: Wenxiang
authors: W
authors: Chen
authors: Shu
authors: S
authors: Wang
authors: Jiangtao
authors: J
authors: Zhang
authors: Hongxia
authors: H
authors: Li
authors: Hanbang
authors: H
authors: Guo
authors: Yong
authors: Y
authors: Man
authors: Baosheng
authors: B
authors: Chen
mesh_terms: D000096:Acetone / Q000737:chemistry; D002784:Cholesterol / Q000032:analysis / Q000097:blood / Q000737:chemistry*; D002840:Chromates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D010084:Oxidation-Reduction; D012015:Reference Standards; D015203:Reproducibility of Results; D013265:Stigmasterol / Q000737:chemistry / Q000592:standards; D013464:Sulfuric Acids / Q000737:chemistry
chemical_list: D002840:Chromates; D013464:Sulfuric Acids; D000096:Acetone; D002784:Cholesterol; D013265:Stigmasterol; C033158:sulfuric acid; C034944:chromic acid