Preparation of a light-sensitive and reversible dissolution copolymer and its application in lysozyme purification.
A novel light-sensitive and cation-exchange copolymer (PNBCC) has been synthesized by random copolymerization of chlorophyllin sodium copper salt, crylic acid, n-butyl acrylate, and N-isopropylacrylamide. The PNBCC copolymer showed reversible dissolution and could be precipitated by 488 nm laser irradiation with the least light density of 1.70 x 10(5) W/m(2). By optimizing the ratio of monomers, pH, and ion concentration, over 95% copolymer was recovered by laser irradiation. The copolymer was used to purify lysozyme as light-sensitive cation exchanger, and its adsorption matched a Langmuir adsorption isotherm with maximum adsorption capacity of 98,900 U/g and dissociated constant of 852 U/mL. By applying the copolymer to the separation of lysozyme from egg white, the specific activity of lysozyme was improved from 399 to 6346 U/mg and the recovery of lysozyme achieved 81.3%.
Wen
Wen
W
State Key Laboratory of Bioreactor Engineering, Department of Biochemical Engineering, East China University of Science & Technology, 130 Meilong Rd., Shanghai, China 200237.
Wan
Junfen
J
Cao
Xuejun
X
Xia
Jinan
J
D001672:Biocompatible Materials / Q000737:chemistry* / Q000528:radiation effects; D008027:Light; D009113:Muramidase / Q000737:chemistry* / Q000302:isolation & purification*; D010777:Photochemistry / Q000379:methods*; D011108:Polymers / Q000737:chemistry* / Q000528:radiation effects*; D011829:Radiation Dosage
D001672:Biocompatible Materials; D011108:Polymers; D009113:Muramidase