Bioaccessibility and antioxidant activity stability of phenolic compounds from extra-virgin olive oils during in vitro digestion.
The impact of an in vitro procedure that mimics the physiochemical changes occurring in gastric and small intestinal digestion on the bioaccessibility and antioxidant activity of phenols from 10 extra-virgin olive oil samples was assessed. Extra-virgin olive oil phenols were totally extracted in the aqueous phase, which reproduces gastric fluids during the digestion procedure. A linear bioaccessibility model, based on tyrosol behavior in model oil samples, was used to estimate the bioaccessibility index (BI%) of extra-virgin olive oil phenols. The BI% varied amongst samples from a maximum of 90% to a minimum of 37%, thus indicating that only a fraction of phenols can be considered bioaccessible. The specific antioxidant activity of olive oil phenols proved to be negatively affected by the digestion procedure. By computing a principal component analysis, it was possible to show that differences in the potential bioactive effect of extra-virgin olive oil samples were related to different phenolic profiles.
Dinnella
Caterina
C
Dept. Biotecnologie Agrarie, Università degli Studi di Firenze, via Donizetti, 6 50144 Firenze, Italy. dinnella@unifi.it
Minichino
Patrizia
P
D'Andrea
Anna Maria
AM
Monteleone
Erminio
E
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001682:Biological Availability; D004063:Digestion; D004355:Drug Stability; D066298:In Vitro Techniques; D000069463:Olive Oil; D010434:Pepsin A / Q000378:metabolism; D010636:Phenols / Q000032:analysis* / Q000378:metabolism* / Q000493:pharmacokinetics; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000378:metabolism; D010938:Plant Oils / Q000737:chemistry*; D013552:Swine; D014867:Water
D000975:Antioxidants; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; D014867:Water; C011867:4-hydroxyphenylethanol; D010434:Pepsin A; D010626:Phenylethyl Alcohol