Simultaneous determination of 1-chloro-2,4-dinitrobenzene, 2,4-dinitrophenyl-S-glutathione and its metabolites for human placental disposition studies by high-performance liquid chromatography.
We developed and validated an HPLC method for determination of 1-chloro-2,4-dinitrobenzene (CDNB) and its glutathione conjugate 2,4-dinitrophenyl-S-glutathione (DNP-SG) to study the kinetics and mechanisms involved in DNP-SG formation and efflux, as a probe for human placental metabolism and transport. This method combines use of 3 microm solid phase, rapid mobile phase gradient with dual wavelength ultraviolet detection to permit determination of a lipophilic parent compound and its hydrophilic metabolites in a single short run. The selectivity, linearity, accuracy, precision, relative recovery and stability of the assay are sufficient for determining CDNB, DNP-SG and its metabolites from buffer and tissue samples to support placental drug metabolism and transport studies.
Vaidya
Soniya S
SS
Virginia Commonwealth University, Medical College of Virginia Campus, Department of Pharmaceutics, Richmond, VA23298-0533, United States.
Gerk
Phillip M
PM
D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004137:Dinitrochlorobenzene / Q000032:analysis* / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012015:Reference Standards; D015203:Reproducibility of Results
D002021:Buffers; D004137:Dinitrochlorobenzene; C030190:S-(2,4-dinitrophenyl)glutathione; D005978:Glutathione