Investigation on clotting and hemolysis characteristics of heparin-immobilized polyether sulfones biomembrane.
In this study, a novel heparin-immobilized polyethersulfone (PES) was synthesized. PES was initially sulfonated with chlorosulfonic acid and then 1,6-hexanediamine was grafted to the -SO(3)H groups of sulfonated PES, which subsequently reacted with heparin through a covalent bond by using (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDAC) as catalyst. The hydrophobic/hydrophilic property was characterized by measuring the water contact angle. The data shows decline from 62.29 degrees +/- 1.2 degrees to 47.86 degrees +/- 0.3 degrees for water and 86.79 degrees +/- 0.8 degrees to 68.34 degrees +/- 1.0 degrees for glycerol, which indicates an enhancement of hydrophilicity. Plasma hemolysis assay shows a comparatively low hemolysis ratio of 1.04%, which is below permissible limit of 5%. A higher content of dissociated blood cells and Ca(2+) concentration was found in red blood cell counting and coagulation factor IV test in heparinized PES. Plasma recalcification time of 360 s also offers positive evidence that heparinized PES seems to have a good anticoagulation property. This new heparin-immobilized PES biomaterials may have the potential for biomedical applications.
Hou
Changjun
C
Department of Chemical Engineering, College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China. houcj@cqu.edu.cn
Yuan
Quan
Q
Huo
Danqun
D
Zheng
Shujia
S
Zhan
Dongli
D
D000818:Animals; D000925:Anticoagulants; D001672:Biocompatible Materials; D001777:Blood Coagulation / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects*; D006493:Heparin / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008567:Membranes, Artificial; D011108:Polymers / Q000737:chemistry*; D055672:Static Electricity; D013450:Sulfones / Q000737:chemistry*
D000925:Anticoagulants; D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; C022840:polyether sulfone; D006493:Heparin