A bioinformatics approach to identifying tail-anchored proteins in the human genome.
Intracellular proteins with a carboxy-terminal transmembrane domain and the amino-terminus oriented toward the cytosol are known as 'tail-anchored' proteins. Tail-anchored proteins have been of considerable interest because several important classes of proteins, including the vesicle-targeting/fusion proteins known as SNAREs and the apoptosis-related proteins of the Bcl-2 family, among others, utilize this unique membrane-anchoring motif. Here, we use a bioinformatic technique to develop a comprehensive list of potentially tail-anchored proteins in the human genome. Our final list contains 411 entries derived from 325 unique genes. We also analyzed both known and predicted tail-anchored proteins with respect to the amino acid composition of the transmembrane segments. This analysis revealed a distinctive composition of the membrane anchor in SNARE proteins.
Kalbfleisch
Ted
T
Department of Biochemistry and Molecular Biology, University of Louisville School of Medicine, Louisville, KY 40202, USA.|Center for Genetics and Molecular Medicine, University of Louisville School of Medicine, Louisville, KY 40202, USA.
Cambon
Alex
A
Department of Bioinformatics and Biostatistics, School of Public Health and Information Sciences, University of Louisville, Louisville, KY 40202, USA.
Wattenberg
Binks W
BW
Department of Biochemistry and Molecular Biology, University of Louisville School of Medicine, Louisville, KY 40202, USA.|Department of Medicine, University of Louisville School of Medicine, Louisville, KY 40202, USA.|Department of Pharmacology and Toxicology, University of Louisville School of Medicine, Louisville, KY 40202, USA.
D000596:Amino Acids / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D003600:Cytosol / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D015894:Genome, Human; D006367:HeLa Cells; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism; D017434:Protein Structure, Tertiary; D040901:Proteomics / Q000379:methods; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism
D000596:Amino Acids; D008565:Membrane Proteins; D019253:Proto-Oncogene Proteins c-bcl-2