Induction of mitochondrial biogenesis is a maladaptive mechanism in mitochondrial cardiomyopathies.
OBJECTIVE
The purpose of this study was to clarify the molecular mechanisms linking human mitochondrial deoxyribonucleic acid (mtDNA) dysfunction to cardiac remodeling.


BACKGROUND
Defects of the mitochondrial genome cause a heterogeneous group of clinical disorders, including mitochondrial cardiomyopathies (MIC). The molecular events linking mtDNA defects to cardiac remodeling are unknown. Energy derangements and increase of mitochondrial-derived reactive oxygen species (ROS) could both play a role in the development of cardiac dysfunction in MIC. In addition, mitochondrial proliferation could interfere with sarcomere alignment and contraction.


METHODS
We performed a detailed morphologic and molecular analysis on failing hearts from 3 patients with MIC, failing human hearts due to ischemic heart disease (IHD) or dilated cardiomyopathies (DCM), and nonfailing hearts.


RESULTS
The MIC hearts showed marked mitochondrial proliferation with myofibril displacement. Consistent with morphologic features, increase in mtDNA content per cell and induction of genes involved in mitochondrial biogenesis, fatty acid metabolism, and glucose transport were observed. Down-regulation of these genes characterized DCM and IHD hearts. A pronounced increase in mitochondrial-derived ROS was observed in MIC hearts compared with failing hearts due to other causes. This was paralleled by up-regulation of genes encoding for uncoupling proteins and antioxidant enzymes. However, there was not a significant increase in antioxidant enzyme activity.


CONCLUSIONS
Our results suggest that besides energy deficiency, mitochondrial biogenesis per se is a maladaptive response in MIC and, possibly, in other metabolic cardiomyopathies.
Sebastiani
Mariangela
M
Dipartimento di Medicina Sperimentale, Polo Pontino, Sapienza, Università di Roma, Rome, Italy.
Giordano
Carla
C
Nediani
Chiara
C
Travaglini
Claudia
C
Borchi
Elisabetta
E
Zani
Massimo
M
Feccia
Mariano
M
Mancini
Massimiliano
M
Petrozza
Vincenzo
V
Cossarizza
Andrea
A
Gallo
Pietro
P
Taylor
Robert W
RW
d'Amati
Giulia
G
D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D009202:Cardiomyopathies / Q000201:enzymology / Q000235:genetics* / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D004734:Energy Metabolism / Q000235:genetics; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D008929:Mitochondria, Heart / Q000201:enzymology / Q000235:genetics* / Q000473:pathology*; D028361:Mitochondrial Diseases / Q000201:enzymology / Q000235:genetics* / Q000473:pathology*; D018384:Oxidative Stress / Q000235:genetics; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D015415:Biomarkers; D013481:Superoxides; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase