title: Endogenous neurogenesis and neovascularization in the neocortex of the rat after focal cerebral ischemia.
abstract: The present study was designed to examine whether endogenous neurogenesis and neovascularization occur in the neocortex of the ischemic rat brain after unilateral middle cerebral artery occlusion (MCAO). Sprague-Dawley rats were divided into six groups (n = 29): one control group (n = 4) and five groups composed of animals sacrificed at increasing times post-MCAO (2 days and 1, 2, 4, and 8 weeks; n = 5 per group). To determine the presence of neurogenesis and neovascularization in the ischemic brain, nestin, Tuj1, NeuN, GFAP, Tie2, RECA, and 5-bromo-2'-deoxyuridine (BrdU) were analyzed immunohistochemically. In addition, nestin, GFAP, and Tie2 expression was determined by Western blotting. Triple-labeling of nestin, BrdU, and laminin was performed to visualize the interaction between endogenous neurogenesis and neovascularization. The number of BrdU- and nestin-colabeled cells increased markedly in the neocortex and border zone of the ischemic area up to 1 week after MCAO and decreased thereafter. Western blot analysis revealed that the expression of nestin, Tie-2, and GFAP was amplified in the ipsilateral hemisphere 2 days after MCAO and peaked 1 week after MCAO, compared with that in the normal brain. After ischemic injury, nestin- and BrdU-colabeled cells were observed in the vicinity of the endothelial cells lining cerebral vessels in the ipsilateral neocortex of the ischemic brain. Endogenous neurogenesis and neovascularization were substantially activated and occurred in close proximity to one other in the ipsilateral neocortex of the ischemic rat brain.
authors: Department of Neurosurgery, Seoul National University College of Medicine, Seoul, Korea.
authors: Hye Young
authors: HY
authors: Shin
authors: Jin Hyun
authors: JH
authors: Kim
authors: Ji Hoon
authors: JH
authors: Phi
authors: Chul-Kee
authors: CK
authors: Park
authors: Jung Eun
authors: JE
authors: Kim
authors: Jong-Hoon
authors: JH
authors: Kim
authors: Sun Ha
authors: SH
authors: Paek
authors: Kyu-Chang
authors: KC
authors: Wang
authors: Dong Gyu
authors: DG
authors: Kim
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002545:Brain Ischemia / Q000473:pathology*; D049109:Cell Proliferation; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008297:Male; D018613:Microscopy, Confocal; D019579:Neocortex / Q000098:blood supply / Q000502:physiology*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin