title: 20-Hydroxyecdysone regulation of two isoforms of the Ets transcription factor E74 gene in programmed cell death in the silkworm anterior silk gland.
abstract: Programmed cell death of larval-specific tissues in insects is under the control of 20-hydroxyecdysone (20E). The ecdysteroid-regulated early genes are conserved in the programmed cell death of anterior silk glands (ASGs) in Bombyx mori and salivary glands in Drosophila melanogaster. We identified and characterized two isoforms of the Ets transcription factor E74 gene in B. mori (BmE74). In ASGs of B. mori last instar larvae, the Bm74A mRNA level increased concomitantly with an increase in haemolymph ecdysteroid titre after gut purge. The optimal 20E concentration for stimulation of Bm74A in ASGs was 4 microM, a similar value to the peak haemolymph ecdysteroid concentration after gut purge. In contrast, BmE74B expression peaked on day 5 of the feeding period, after which it did not increase again. These findings suggest that the BmE74 isoforms play different roles in the regulation of programmed cell death in B. mori ASGs.
authors: Division of Life Sciences, Graduate School of Science and Technology, Kanazawa University, Kakumamachi, Kanazawa, Japan.
authors: T
authors: T
authors: Sekimoto
authors: M
authors: M
authors: Iwami
authors: S
authors: S
authors: Sakurai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000235:genetics / Q000502:physiology*; D016923:Cell Death / Q000502:physiology*; D004441:Ecdysterone / Q000502:physiology*; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000502:physiology; D017434:Protein Structure, Tertiary; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D020033:Protein Isoforms; D014157:Transcription Factors; D004441:Ecdysterone