Multiple Mycobacterium tuberculosis infections in an HIV-infected patient.
Mycobacterial colonies of two different morphologies were isolated from one sputum sample of a HIV-positive patient. One morphological type was resistant to streptomycin (STR) and susceptible to isoniazid (INH), while the other isolate with different colony morphology was resistant to both of these anti-TB drugs. A mycobacterial isolate of one pus from a lymph node sample was resistant to these two anti-TB drugs, while the other isolate from another pus sample was resistant to STR but susceptible INH. IS6110 RFLP based finger printing revealed that the HIV-positive patient was infected with different strains of Mycobacterium tuberculosis. A subculture of isolates on solid medium is useful to examine mixed infection.
van der Zanden
Adri G M
AG
Department of Medical Microbiology and Infection Prevention, Location Lukas, Gelre Hospitals, Apeldoorn, the Netherlands. agm.vd.zanden@gelre.nl
Rahim
Zeaur
Z
Fedder
Guido
G
Vos-van Adrichem
Janine
J
Sebens
Fré W
FW
Heilmann
Frank G C
FG
van Soolingen
Dick
D
D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology*; D000328:Adult; D000995:Antitubercular Agents / Q000494:pharmacology; D016172:DNA Fingerprinting; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology; D008297:Male; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D009426:Netherlands; D013183:Sputum / Q000382:microbiology; D018088:Tuberculosis, Multidrug-Resistant / Q000235:genetics*
D000995:Antitubercular Agents