title: Echinococcus granulosus: the establishment of the metacestode is associated with control of complement-mediated early inflammation.
abstract: In this work we studied the evolution of early inflammation, complement activation and parasite survival/death along the establishment phase of Echinococcus granulosus metacestode. Using a chamber model of infection in mice, we examined cell infiltration and C3 deposition on individual parasites during their development from protoscoleces to cystic forms. We found that the intensity of the initial inflammation decreased around undamaged but not around damaged parasites: at 43dpi undamaged parasites were mostly associated with poor/mild inflammation while damaged parasites with a strong inflammation. In addition, whereas complement activation participated in the induction of early inflammation, the deposition of C3 on undamaged parasites progressively diminished. Interestingly, we observed some parasites in pre-cystic stage with no/poor C3 deposition at 3dpi. Overall, these results indicated that the establishment and survival of the hydatid cyst is associated with the control of complement and, consequently, of local inflammation at the initial phases of infection.
authors: Departamento de Ciencias Microbiológicas, Area Inmunología, Facultad de Veterinaria, Universidad de la República, A. Lasplaces 1620, Montevideo CP11600, Uruguay.
authors: Martin
authors: M
authors: Breijo
authors: Gabriel
authors: G
authors: Anesetti
authors: Laura
authors: L
authors: Martínez
authors: Robert B
authors: RB
authors: Sim
authors: Ana M
authors: AM
authors: Ferreira
mesh_terms: D000818:Animals; D002417:Cattle; D003167:Complement Activation / Q000502:physiology*; D003176:Complement C3 / Q000032:analysis / Q000276:immunology*; D015194:Diffusion Chambers, Culture; D004195:Disease Models, Animal; D015536:Down-Regulation; D004443:Echinococcosis / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D048209:Echinococcus granulosus / Q000254:growth & development / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D007150:Immunohistochemistry; D007249:Inflammation / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D003176:Complement C3; D007074:Immunoglobulin G; D007075:Immunoglobulin M