Evaluation of mutagenicity and antimutagenicity of cashew stem bark methanolic extract in vitro.
Plants remain to be a great source of medicines for humanity. Among them is the Anacardium occidentale L. species, popularly known as the cashew, which has several therapeutic indications, such as cicatrizing, antihypertensive, hypoglycemic and antitumoral properties. The objective of the present work was to assess the mutagenicity and antimutagenicity of cashew stem bark methanolic extract (CSBME) on cell cultures of Chinese hamster lung fibroblasts (V79). The cultures were treated with different concentrations of the CSBME (500, 1000 and 2000 microg/mL) or with the extract associated with doxorubicin (DXR, 0.75 microg/mL) during the cell cycle phases G1, S, and G2 and throughout continuous treatment. Apart from these treatments, the cell cultures were also treated with PBS (negative-control) and DXR (positive-control). The data obtained in the chromosome aberrations (CA) test showed a significant reduction in CA frequency in the cultures treated with DXR and extract in comparison with those that received only DXR during the cell cycle phases G1 and S and throughout the entire cycle, as well as the absence of mutagenicity in all the treatments realized. The antimutagenic effect observed in this work reinforces the presence of the previously described therapeutic properties of cashew and indicates the safe use of this extract.
Barcelos
Gustavo Rafael Mazzaron
GR
Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid km 380, 86051-990 Londrina, Paraná, Brazil.
Shimabukuro
Fernanda
F
Mori
Mateus Prates
MP
Maciel
Maria Aparecida Medeiros
MA
Cólus
Ilce Mara de Syllos
IM
D031021:Anacardium / Q000737:chemistry* / Q000633:toxicity; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002869:Chromosome Aberrations / Q000187:drug effects; D006224:Cricetinae; D004317:Doxorubicin / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D000432:Methanol; D008940:Mitotic Index; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D024301:Plant Bark / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012997:Solvents
D000903:Antibiotics, Antineoplastic; D016587:Antimutagenic Agents; D009153:Mutagens; D012997:Solvents; D004317:Doxorubicin; D000432:Methanol