title: Increased expression in calcitonin-like receptor induced by aldosterone in cerebral arteries from spontaneously hypertensive rats does not correlate with functional role of CGRP receptor.
abstract: OBJECTIVE
  To analyze the effect of aldosterone on the expression of calcitonin gene-related peptide (CGRP) receptor components, calcitonin-like receptor (CL receptor) and receptor activity modifying protein 1 (RAMP1), as well as the effect of this mineralocorticoid on CGRP-mediated vasodilation in middle cerebral arteries from Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR).
  
  
  RESULTS
  CGRP 0.1 nM-0.1 microM induced a concentration-dependent relaxation that was nitric oxide independent and higher in SHR middle cerebral arteries. CL receptor and RAMP1 expression were similar in both strains. The relaxation to CGRP was not modified by aldosterone 1 microM in either strain, although aldosterone 1 microM increased expression of CL receptor without modifying RAMP1 in segments from SHR rats.
  
  
  CONCLUSIONS
  CGRP elicits greater vasodilation in middle cerebral arteries from SHR than WKY rats, that is nitric oxide independent, and by mechanism independent of CGRP receptor components expression. Although aldosterone increases the expression of CL receptor in SHR, it does not alter vasodilation to CGRP, since RAMP1 expression is not increased. These results indicate that the increase in CL receptor, without an increase in RAMP1, does not correlate with changes in functional role of the CGRP receptor.
authors: Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, Spain.
authors: Iván
authors: I
authors: Márquez-Rodas
authors: Fabiano E
authors: FE
authors: Xavier
authors: Irene
authors: I
authors: Arroyo-Villa
authors: Federico
authors: F
authors: Longo
authors: Rosa
authors: R
authors: Aras-Lopez
authors: Javier
authors: J
authors: Blanco-Rivero
authors: Mercedes
authors: M
authors: Ferrer
authors: Gloria
authors: G
authors: Balfagón
mesh_terms: D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D058286:Calcitonin Receptor-Like Protein; D002536:Cerebral Arteries / Q000187:drug effects* / Q000378:metabolism; D006973:Hypertension; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D058261:Receptor Activity-Modifying Proteins; D018003:Receptors, Calcitonin / Q000187:drug effects / Q000378:metabolism*; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D058286:Calcitonin Receptor-Like Protein; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D058261:Receptor Activity-Modifying Proteins; D018003:Receptors, Calcitonin; D018015:Receptors, Calcitonin Gene-Related Peptide; D000450:Aldosterone