Authentic seed-specific activity of the Perilla oleosin 19 gene promoter in transgenic Arabidopsis.
The Perilla (Perilla frutescens L. cv. Okdong) oleosin gene, PfOle19, produces a 19-kDa protein that is highly expressed only in seeds. The activity of the -2,015 bp 5'-upstream promoter region of this gene was investigated in transgenic Arabidopsis plants using the fusion reporter constructs of enhanced green fluorescent protein (EGFP) and beta-glucuronidase (GUS). The PfOle19 promoter directs Egfp expression in developing siliques, but not in leaves, stems or roots. In the transgenic Arabidopsis, EGFP fluorescence and histochemical GUS staining were restricted to early seedlings, indehiscent siliques and mature seeds. Progressive 5'-deletions up to the -963 bp position of the PfOle19 promoter increases the spatial control of the gene expression in seeds, but reduces its quantitative levels of expression. Moreover, the activity of the PfOle19 promoter in mature seeds is 4- and 5-fold greater than that of the cauliflower mosaic virus 35S promoter in terms of both EGFP intensity and fluorometric GUS activity, respectively.
Chung
Kyoung-Ji
KJ
National Institute of Agricultural Biotechnology, RDA, 225 Seodun-dong, Suwon, 441-707, South Korea.
Hwang
Seon-Kap
SK
Hahn
Bum-Soo
BS
Kim
Kyung-Hwan
KH
Kim
Jong-Bum
JB
Kim
Yong-Hwan
YH
Yang
Joo-Sung
JS
Ha
Sun-Hwa
SH
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D031343:Perilla / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics* / Q000378:metabolism
D049452:Green Fluorescent Proteins