title: Pathology underrates colon cancer extranodal and nodal metastases; ex vivo radioimmunodetection helps staging.
abstract: OBJECTIVE
  Colorectal carcinoma is frequently accompanied by small lymph nodes metastases that often escape pathologic examination. We evaluated whether ex vivo radioimmunodetection with the Affinity Enhancement System (AES) could improve detection of mesocolonic metastases.
  
  
  METHODS
  A bivalent 111In-labeled hapten was injected (16 patients) 4 days after a bispecific antibody (anticarcinoembryonic antigen, antihapten). Surgery was done 1 to 3 days later, and radioactive uptake in the mesocolon was recorded. Extensive pathologic examination of the mesocolon (reference method) was done after fat dissolution. This method visualizes all lymph nodes but is not in routine use.
  
  
  RESULTS
  The reference method disclosed 705 nodes. There was no significant difference between the number of node metastases detected by AES or by the reference method (16 versus 17). Better detection would have been obtained by AES than by routine pathology (P<0.01). In addition 12 extranodal metastases were found in this study of which eight were detected by AES. The prognostic importance of such extranodal metastases has been underlined in the literature. Routine pathology combined with AES would have disclosed all node metastases and 86% of total metastases versus 35% by routine pathology alone.
  
  
  CONCLUSIONS
  Ex vivo radioimmunodetection could improve nodal and extranodal metastases detection in patients with colorectal cancer. Its value for improving pathologic analysis, together with the effect of these small metastases on prognosis, should be further evaluated. The benefit of adjuvant chemotherapy for patients upstaged with radioimmunodection should also be assessed because adjuvant chemotherapy improves the 5-year survival of stage III patients.
authors: Service de Chirurgie Digestive et Générale, Groupe Hospitalier Diaconesses-Croix Saint Simon, and Université Pierre et Marie Curie-Paris 6, France. asezeur@hopital-dcss.org
authors: A
authors: A
authors: Sézeur
authors: F P
authors: FP
authors: Châtelet
authors: Ch
authors: Ch
authors: Cywiner
authors: Cl
authors: C
authors: de Labriolle-Vaylet
authors: C
authors: C
authors: Chastang
authors: Cl
authors: C
authors: Billotey
authors: M
authors: M
authors: Malafosse
authors: D
authors: D
authors: Gallot
authors: P
authors: P
authors: Betton
authors: F
authors: F
authors: Montravers
authors: S
authors: S
authors: Carvajal-Gonzalez
authors: S
authors: S
authors: Askienazy
authors: J N
authors: JN
authors: Talbot
authors: J D
authors: JD
authors: Rain
authors: G
authors: G
authors: Milhaud
authors: G
authors: G
authors: Saumon
authors: J
authors: J
authors: Barbet
authors: A
authors: A
authors: Gruaz-Guyon
mesh_terms: D000230:Adenocarcinoma / Q000000981:diagnostic imaging* / Q000556:secondary / Q000601:surgery; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018033:Antibodies, Bispecific; D002272:Carcinoembryonic Antigen / Q000276:immunology; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology / Q000601:surgery; D006241:Haptens; D006801:Humans; D007205:Indium Radioisotopes; D008198:Lymph Nodes; D008207:Lymphatic Metastasis / Q000000981:diagnostic imaging; D008875:Middle Aged; D009367:Neoplasm Staging; D009842:Oligopeptides / Q000737:chemistry; D011379:Prognosis; D016719:Radioimmunodetection
chemical_list: D018033:Antibodies, Bispecific; D002272:Carcinoembryonic Antigen; D006241:Haptens; D007205:Indium Radioisotopes; D009842:Oligopeptides