Ectopic expression of a hyacinth AGL6 homolog caused earlier flowering and homeotic conversion in Arabidopsis.
MADS-box genes are involved in floral organ development. Here we report that an AGL6(Agamous-like 6)-like MADS-box gene, HoAGL6, was isolated from Hyacinthus orientalis L. Expression pattern analysis demonstrated that HoAGL6 transcript was detected in inflorescence buds, tepals, carpels and ovules, but not in stamina, leaves or scales. Transgenic Arabidopsis plants ectopically expressing HoAGL6 exhibited novel phenotypes of significantly reduced plant size, extremely early flowering, and losing inflorescence indeterminacy. In addition, wide homeotic conversion of sepals, petals, and leaves into carpel-like or ovary structures, and disappearance or number reduction of stamens in 35S::HoAGL6 Arabidopsis plants were also observed. RT-PCR analysis indicated that the expressions of flowering time gene SOC1 and flower meristem identity gene LFY were significantly up-regulated in 35S::HoAGL6 transgenic Arabidopsis plants, and the expression levels of floral organ identity genes AG and SEP1 in leaves were also elevated. These results indicated that HoAGL6 was involved in the regulation of flower transition and flower organ formation.
Fan
JinHui
J
College of Forestry, Shandong Agricultural University, Taian 271018, China. hjf2003@sdau.edu.cn
Li
WenQing
W
Dong
XiuChun
X
Guo
Wei
W
Shu
HuaiRui
H
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D015139:Blotting, Southern; D035264:Flowers / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D031423:Hyacinthus; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D010940:Plant Proteins