title: Bivalent peptides as PDZ domain ligands.
abstract: A series of multivalent peptides, with the ability to simultaneously bind two separate PDZ domain proteins, has been designed, synthesized, and tested by isothermal titration calorimetry (ITC). The monomer sequences, linked with succinate, varied in length from five to nine residues. The thermodynamic binding parameters, in conjunction with results from mass spectrometry, indicate that a ternary complex is formed in which each peptide arm binds two equivalents of the third PDZ domain (PDZ3) of the neuronal protein PSD-95.
authors: Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
authors: Edvin
authors: E
authors: Klosi
authors: Dorina
authors: D
authors: Saro
authors: Mark R
authors: MR
authors: Spaller
mesh_terms: D000595:Amino Acid Sequence; D008024:Ligands; D008969:Molecular Sequence Data; D054731:PDZ Domains; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D013816:Thermodynamics
chemical_list: D008024:Ligands; D010455:Peptides