Processed bovine dentine as a bone substitute.
OBJECTIVE
Different forms of allogenic dentine have been studied for their potential use as bone substitutes. We report a new method for processing bovine dentine that results in a sterile bioactive material for repair and regeneration of bone.


METHODS
Extracted bovine dentine was processed mechanically and chemically with inorganic and organic solvents, and sterilised. The in vitro biocompatibility on human gingival fibroblasts was assessed by the Alamar Blue assay and the in vivo biocompatibility evaluated by implantation of the processed dentine into rats' femurs.


RESULTS
The dentine showed excellent biocompatibility in vitro, stimulated formation of new bone and was completely incorporated into the new bone in vivo.


CONCLUSIONS
Processed bovine dentine has the potential to be used as a suitable substitute in bone repair and regeneration.
Moharamzadeh
Keyvan
K
School of Clinical Dentistry, University of Sheffield, Claremont Crescent, Sheffield S10 2TA, UK. k.moharamzadeh@sheffield.ac.uk
Freeman
Christine
C
Blackwood
Keith
K
D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000633:toxicity; D002417:Cattle; D002478:Cells, Cultured; D003804:Dentin; D005269:Femur; D005347:Fibroblasts / Q000187:drug effects; D006652:Histological Techniques; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D010078:Oxazines; D051381:Rats; D014966:Xanthenes
D001672:Biocompatible Materials; D018786:Bone Substitutes; D007202:Indicators and Reagents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin