Histopathology and immunohistochemistry in distinguishing Burkitt lymphoma from diffuse large B-cell lymphoma with very high proliferation index and with or without a starry-sky pattern: a comparative study with EBER and FISH.
Burkitt lymphoma (BL) is characterized by c-myc translocation and CD10+/bc-2-/bcl-6+ with a very high Ki-67 proliferation index (PI). Occasional diffuse large B-cell lymphomas may exhibit a very high PI with or without a starry-sky pattern (DLBCL-HPSS). We compared 28 consecutive BL and 16 DLBCL-HPSS cases in immunocompetent Taiwanese diagnosed by histopathologic examination and immunophenotyping and compared the results with results for Epstein-Barr virus-encoded messenger RNA (EBER) and fluorescence in situ hybridization (FISH). There were statistically significant differences in the expression of CD10 (28/28 vs 1/16), bcl-2 (3/28 vs 11/16), MUM1 (5/28 vs 15/16), a PI of 95.0% or more (27/28 vs 2/16), and combined CD10+/bcl-2-/bcl-6+ (24/28 vs 1/16) between BLs and DLBCL-HPSSs. Of the BLs, 7 (25%) of 28 and 26 (96%) of 27 were positive for EBER and c-myc rearrangement as compared with 0 of 16 and 1 (7%) of 15 DLBCL-HPSSs, respectively. We can confidently distinguish BL from DLBCL-HPSS by using histopathologic and immunohistochemical (CD10, bcl-2, bcl-6, Ki-67) methods without the aid of EBER and FISH in the great majority of cases.
Chuang
Shih-Sung
SS
Department of Pathology, Chi-Mei Medical Center, Tainan Departments of Pathology, Taipei Medical University, Taipei, Taiwan.
Ye
Hongtao
H
Du
Ming-Qing
MQ
Lu
Chin-Li
CL
Dogan
Ahmet
A
Hsieh
Pin-Pen
PP
Huang
Wan-Ting
WT
Jung
Yun-Chih
YC
D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis / Q000276:immunology; D002051:Burkitt Lymphoma / Q000737:chemistry / Q000175:diagnosis* / Q000276:immunology; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D003937:Diagnosis, Differential; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D016393:Lymphoma, B-Cell / Q000737:chemistry / Q000175:diagnosis* / Q000276:immunology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000737:chemistry / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis*
D014408:Biomarkers, Tumor; D012367:RNA, Viral