Purification of PEGylated nanoparticles using tangential flow filtration (TFF).
A tangential flow filtration system was evaluated to purify PEGylated nanoparticles. Two widely used surfactants, PVA and sodium cholate were efficiently removed from an empty nanoparticles suspension using the proposed system. During drug loading, surfactant (PVA) was observed to be entrapped within the core of the nanoparticle to a higher extent, hence was purified at a comparatively slower rate. The presence of dextran sulfate enhanced the drug loading but also resulted in reduced purification rate; this was described by the hypothesis of PVA inclusion within the core of the nanoparticles. Practically, it was possible to correlate the slow purification rate of PVA to its reduced filtration flow during the purification of the empty and loaded nanoparticles containing dextran sulfate. Indirectly, this system was capable of revealing the influence of an excipient and drug on the nanoparticle surface.
Dalwadi
Gautam
G
School of Pharmacy, Curtin University of Technology, Bentley Campus, Bentley, Perth, WA, Australia. gdalwadi@hotmail.com
Sunderland
Vivian Bruce
VB
D016264:Dextran Sulfate / Q000737:chemistry; D005079:Excipients / Q000737:chemistry*; D005374:Filtration; D019344:Lactic Acid / Q000737:chemistry; D008139:Loperamide / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D020358:Sodium Cholate / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry
D005079:Excipients; D011108:Polymers; D013501:Surface-Active Agents; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols; D008139:Loperamide; D011142:Polyvinyl Alcohol; D016264:Dextran Sulfate; D020358:Sodium Cholate