title: Spontaneously immortalized T lymphocytes from Nijmegen Breakage Syndrome patients display phenotypes typical for lymphoma cells.
abstract: We found that the peripheral T lymphocytes from four of eight patients with the lymphoma predisposing Nijmegen Breakage Syndrome (NBS) acquired an unlimited growth potential following in vitro mitogen stimulation and subsequent interleukin-2-dependent propagation. The immortal T cell lines revealed morphological and other features typical for anaplastic large cell lymphoma (ALCL). In addition, multiple copies of ALK, but with no ALK gene rearrangements were found in a subpopulation of cells of one of the immortalized lines. These cell lines may be useful for the in vitro elucidation of mechanisms involved in the development of ALCL.
authors: Department of Immunology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, 02-781 Warsaw, Roentgena 5, Poland. jksiwicki@coi.waw.pl
authors: Jan Konrad
authors: JK
authors: Siwicki
authors: Grzegorz
authors: G
authors: Rymkiewicz
authors: Katarzyna
authors: K
authors: Błachnio
authors: Jolanta
authors: J
authors: Rygier
authors: Piotr
authors: P
authors: Kuźniar
authors: Rafał
authors: R
authors: Płoski
authors: Aleksandra
authors: A
authors: Janusz
authors: Henryk
authors: H
authors: Skurzak
authors: Krystyna
authors: K
authors: Chrzanowska
authors: Jan
authors: J
authors: Steffen
mesh_terms: D000077548:Anaplastic Lymphoma Kinase; D002461:Cell Line, Transformed / Q000473:pathology; D002478:Cells, Cultured; D005434:Flow Cytometry; D015321:Gene Rearrangement; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D017728:Lymphoma, Large-Cell, Anaplastic / Q000235:genetics / Q000473:pathology*; D049932:Nijmegen Breakage Syndrome / Q000473:pathology*; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D020794:Receptor Protein-Tyrosine Kinases; D013601:T-Lymphocytes / Q000473:pathology*
chemical_list: C000626173:ALK protein, human; D000077548:Anaplastic Lymphoma Kinase; D011505:Protein-Tyrosine Kinases; D020794:Receptor Protein-Tyrosine Kinases