title: Characterization of the N-terminal segment used by the barley yellow dwarf virus movement protein to promote interaction with the nuclear membrane of host plant cells.
abstract: The barley yellow dwarf virus movement protein (BYDV-MP) requires its N-terminal sequence to promote the transport of viral RNA into the nuclear compartment of host plant cells. Here, graphical analysis predicts that this sequence would form a membrane interactive amphiphilic alpha-helix. Confirming this prediction, NT1, a peptide homologue of the BYDV-MP N-terminal sequence, was found to be alpha-helical (65%) in the presence of vesicles mimics of the nuclear membrane. The peptide increased the fluidity of these nuclear membrane mimics (rise in wavenumber of circa 0.5-1.0 cm(-1)) and induced surface pressure changes of 2 mN m(-1) in lipid monolayers with corresponding compositions. Taken with isotherm analysis these results suggest that BYDV-MP forms an N-terminal amphiphilic alpha-helix, which partitions into the nuclear membrane primarily through thermodynamically stable associations with the membrane lipid headgroup region. We speculate that these associations may play a role in targeting of the nuclear membrane by BYDM-MP.
authors: Faculty of Science and Technology, University of Central Lancashire, Preston, UK.
authors: Sarah Rachel
authors: SR
authors: Dennison
authors: Frederick
authors: F
authors: Harris
authors: Klaus
authors: K
authors: Brandenburg
authors: David Andrew
authors: DA
authors: Phoenix
mesh_terms: D001467:Hordeum / Q000166:cytology / Q000378:metabolism / Q000821:virology; D008055:Lipids / Q000737:chemistry; D017793:Luteovirus / Q000378:metabolism*; D009685:Nuclear Envelope / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D053830:Plant Viral Movement Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D008055:Lipids; D010455:Peptides; D053830:Plant Viral Movement Proteins