CTX-M extended-spectrum beta-lactamases among clinical isolates of Enterobacteriaceae in a Thai university hospital.
This study presents updates on molecular epidemiology of extended-spectrum beta-lactamases (ESBLs) in clinical isolates of Enterobacteriaceae from Srinagarind Hospital, Khon Kaen University, Thailand. All isolates were screened for the presence of ESBL genes, bla(TEM), bla(SHV), bla(VEB) and bla(CTX-M), using PCR followed by nucleotide sequence determination. The results revealed that beta-lactamase genes among 48 isolates collected between 1998 and 1999 were bla(SHV) (79%), bla(CTX-M-9) (52%), bla(TEM-1) (48%) and bla(VEB) (33%), whereas those found in 52 isolates collected in 2003 were bla(TEM-1) (79%), bla(CTX-M-15) (44%), bla(SHV) (36%), bla(VEB) (36%), bla(CTX -M-14) (11%) and bla(CTX-M-9) (10%). In addition, 45 isolates carried at least two different ESBL genes. Using PCR, part of insertion sequence ISEcpl was found in the upstream regions of bla(CTX-M-14) and bla(CTX-M-15). ERIC-PCR analysis revealed that most ESBL-producing isolates were of different strains. This is the first report of CTX-M-9, CTX-M-14 and CTX-M-15 beta-lactamase genes in Enterobacteriaceae in Thailand.
Chanawong
Aroonwadee
A
Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand. aroonwad@kku.ac.th
Lulitanond
Aroonlug
A
Kaewkes
Wanlop
W
Lulitanond
Viraphong
V
Srigulbutr
Sukanya
S
Homchampa
Preecha
P
D000900:Anti-Bacterial Agents; D002511:Cephalosporins / Q000627:therapeutic use; D004755:Enterobacteriaceae / Q000235:genetics* / Q000302:isolation & purification*; D005820:Genetic Testing; D006785:Hospitals, University; D006801:Humans; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D013785:Thailand; D001618:beta-Lactamases / Q000235:genetics*
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D001618:beta-Lactamases