Nasal absorption enhancement of insulin using PEG-grafted chitosan nanoparticles.
The objective of this work was to explore the potential of polyethylene glycol-grafted chitosan (PEG-g-chitosan) nanoparticles as a system for improving the systemic absorption of insulin following nasal administration. Insulin-loaded PEG-g-chitosan nanoparticles were prepared by the ionotropic gelation of PEG-g-chitosan solution using tripolyphosphate ions as the crosslinking agent. The nanoparticles were in the size range 150-300 nm, had a positive electrical charge (+16 to +30 mV) and were associated with insulin (loading efficiency 20-39%). The physicochemical properties of nanoparticles were affected by the composition of the copolymer. In vitro insulin release studies showed an initial burst followed by a slow release of insulin. Intranasal administration of PEG-g-chitosan nanoparticles in rabbits enhanced the absorption of insulin by the nasal mucosa to a greater extent than a suspension of insulin-PEG-g-chitosan and control insulin solution. PEG-g-chitosan nanoparticles are promising vehicles for insulin transport through the nasal mucosa.
Zhang
Xinge
X
The Key Laboratory of Functional Polymer Materials of Ministry Education, Institute of Polymer Chemistry, Nankai University, Tianjin, China.
Zhang
Huijie
H
Wu
Zhongming
Z
Wang
Zhen
Z
Niu
Haimei
H
Li
Chaoxing
C
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002626:Chemistry, Pharmaceutical; D048271:Chitosan / Q000008:administration & dosage*; D005260:Female; D007328:Insulin / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D053758:Nanoparticles / Q000008:administration & dosage*; D009297:Nasal Mucosa / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000008:administration & dosage*; D011817:Rabbits; D012995:Solubility
D001786:Blood Glucose; D007328:Insulin; D011092:Polyethylene Glycols; D048271:Chitosan