Effects of a plasmid-encoded qnrA1 determinant in Escherichia coli strains carrying chromosomal mutations in the acrAB efflux pump genes.
The qnrA1 gene was cloned into a plasmid vector and transformed into Escherichia coli strains carrying chromosomal mutations in the acrAB drug efflux system. In acrAB-deleted strains, and in strains overexpressing acrAB, viable qnrA conferred higher MIC levels (at least a 32-fold increase) for fluoroquinolones than were observed in the parent strains.
Jeong
Jin-Yong
JY
Division of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea.
Kim
Eun Sil
ES
Choi
Sang-Ho
SH
Kwon
Hyun-Hee
HH
Lee
Sang-Rok
SR
Lee
Sang-Oh
SO
Kim
Mi-Na
MN
Woo
Jun Hee
JH
Kim
Yang Soo
YS
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D024841:Fluoroquinolones / Q000494:pharmacology; D017353:Gene Deletion; D018628:Gene Dosage; D008074:Lipoproteins / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics*; D010957:Plasmids
D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D024841:Fluoroquinolones; D008074:Lipoproteins; D026901:Membrane Transport Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C083497:AcrA protein, E coli; C083496:AcrB protein, E coli; C485384:Qnr protein, E coli