title: Experimental study on predicting skin flap necrosis by fluorescence in the FAD and NADH bands during surgery.
abstract: The objective of the present study was to assess the feasibility of using endogenous fluorescence in intraoperative prediction of skin flap necrosis. The investigation was carried out in 10 Sprague-Dawley rats in which proximally based over-dimensioned random pattern skin flaps were dissected on the back and thereafter fixed into position. Immediately after surgery on each rat, synchronous fluorescence spectra (Deltalambda=90 nm) from five parts of the skin flap surface were measured. The presence of necrosis was evaluated on postoperative day 7. In flap parts designated as necrotic (n=18), a significantly lower (P<0.001) fluorescent signal from the nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) bands (360-380 and 440-460 nm, respectively) was detected in comparison with the vital parts (n=32) (for FAD:1767+/-39 versus 2579+/-65 auxiliary units [A.U]. and for NADH:11724+/-340 versus 16810+/-473 A.U.). The results suggested a close relationship between the fluorescent signals from the FAD and NADH bands on one side and flap necrosis or survival on the other side. Thus, the use of fluorescence spectroscopy may be considered as a valuable noninvasive tool for the prediction of skin flap necrosis.
authors: Department of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Safárik University in Kosice, Slovak Republic. mmokry@upjs.sk
authors: Michal
authors: M
authors: Mokrý
authors: Peter
authors: P
authors: Gál
authors: Magdaléna
authors: M
authors: Harakalová
authors: Zaneta
authors: Z
authors: Hutnanová
authors: Jaroslav
authors: J
authors: Kusnír
authors: Stefan
authors: S
authors: Mozes
authors: Ján
authors: J
authors: Sabo
mesh_terms: D000818:Animals; D062109:Dermatologic Surgical Procedures; D005260:Female; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry*; D009243:NAD / Q000737:chemistry*; D009336:Necrosis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000473:pathology*; D013050:Spectrometry, Fluorescence; D013524:Surgical Flaps
chemical_list: D009243:NAD; D005182:Flavin-Adenine Dinucleotide