Wheat germ agglutinin-modified trifunctional nanospheres for cell recognition.
A simple and convenient strategy has been put forward to fabricate smart fluorescent magnetic wheat germ agglutinin-modified trifunctional nanospheres (WGA-TFNS) for recognition of human prostate carcinoma DU-145 cells which are surface-expressed with sialic acid and N-acetylglucosamine. These TFNS can be easily manipulated, tracked, and conveniently used to capture and separate target cells. The presence of wheat germ agglutinin on the surface of WGA-TFNS was confirmed by FTIR, biorecognition of carboxymethyl chitin-modified quantum dots (CM-CT-QDs), and bacterium Staphylococcus aureus. The success in recognizing DU-145 cells by the WGA-TFNS indicates that WGA-TFNS could be applicable.
Xie
Hai-Yan
HY
School of Life Science and Technology, Beijing Institute of Technology, Beijing, PR China.
Xie
Min
M
Zhang
Zhi-Ling
ZL
Long
Yan-Min
YM
Liu
Xin
X
Tang
Ming-Liang
ML
Pang
Dai-Wen
DW
Tan
Zheng
Z
Dickinson
Calum
C
Zhou
Wuzong
W
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D008297:Male; D046529:Microscopy, Electron, Transmission; D054118:Nanospheres / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D011471:Prostatic Neoplasms / Q000473:pathology; D017550:Spectroscopy, Fourier Transform Infrared; D014909:Wheat Germ Agglutinins / Q000737:chemistry*
D014909:Wheat Germ Agglutinins