A hybrid FIA/HPLC system incorporating monolithic column chromatography.
We have combined the generation of solvent gradients using milliGAT pumps, chromatographic separations with monolithic columns and chemiluminescence detection in an instrument manifold that approaches the automation and separation efficiency of HPLC, whilst maintaining the positive attributes of flow injection analysis (FIA), such as manifold versatility, speed of analysis and portability. As preliminary demonstrations of this hybrid FIA/HPLC system, we have determined six opiate alkaloids (morphine, pseudomorphine, codeine, oripavine, ethylmorphine and thebaine) and four biogenic amines (vanilmandelic acid, serotonin, 5-hydroxyindole-3-acetic acid and homovanillic acid) in human urine, using tris(2,2'-bipyridyl)ruthenium(III) and acidic potassium permanganate chemiluminescence detection.
Adcock
Jacqui L
JL
School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3217, Australia.
Francis
Paul S
PS
Agg
Kent M
KM
Marshall
Graham D
GD
Barnett
Neil W
NW
D000470:Alkaloids / Q000032:analysis / Q000737:chemistry; D000588:Amines / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D018377:Neurotransmitter Agents / Q000378:metabolism
D000470:Alkaloids; D000588:Amines; D018377:Neurotransmitter Agents