title: 8-plex quantitation of changes in cerebrospinal fluid protein expression in subjects undergoing intravenous immunoglobulin treatment for Alzheimer's disease.
abstract: An 8-plex version of an isobaric reagent for the quantitation of proteins using shotgun methods is presented. The 8-plex version of the reagent relies on amine-labeling chemistry of peptides similar to 4-plex reagents. MS/MS reporter ions at 113, 114, 115, 116, 117, 118, 119, and 121 m/z are used to quantify protein expression. This technology which was first applied to a test mixture consisting of eight proteins and resulted in accurate quantitation, has the potential to increase throughput of analysis for quantitative shotgun proteomics experiments when compared to 2- and 4-plex methods. The technology was subsequently applied to a longitudinal study of cerebrospinal fluid (CSF) proteins from subjects undergoing intravenous Ig treatment for Alzheimer's disease. Results from this study identify a number of protein expression changes that occur in CSF after 3 and 6 months of treatment compared to a baseline and compared to a drug washout period. A visualization tool was developed for this dataset and is presented. The tool can aid in the identification of key peptides and measurements. One conclusion aided by the visualization tool is that there are differences in considering peptide-based observations versus protein-based observations from quantitative shotgun proteomics studies.
authors: School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY, USA.
authors: Leila
authors: L
authors: Choe
authors: Mark
authors: M
authors: D'Ascenzo
authors: Norman R
authors: NR
authors: Relkin
authors: Darryl
authors: D
authors: Pappin
authors: Philip
authors: P
authors: Ross
authors: Brian
authors: B
authors: Williamson
authors: Steven
authors: S
authors: Guertin
authors: Patrick
authors: P
authors: Pribil
authors: Kelvin H
authors: KH
authors: Lee
mesh_terms: D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000276:immunology / Q000628:therapy*; D000595:Amino Acid Sequence; D002256:Carbonic Anhydrases / Q000096:biosynthesis / Q000134:cerebrospinal fluid / Q000235:genetics; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis* / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D016756:Immunoglobulins, Intravenous / Q000008:administration & dosage / Q000627:therapeutic use*; D007202:Indicators and Reagents; D007262:Infusions, Intravenous; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D040901:Proteomics / Q000295:instrumentation / Q000379:methods
chemical_list: D002556:Cerebrospinal Fluid Proteins; D016756:Immunoglobulins, Intravenous; D007202:Indicators and Reagents; D002256:Carbonic Anhydrases