Knock-in of the Oas1b(r) allele into a flavivirus-induced disease susceptible mouse generates the resistant phenotype.
Inheritance patterns in mice suggested that resistance to flavivirus-induced disease was conferred by a single autosomal dominant allele (Flv(r)). A positional cloning strategy followed by comparison of Flv interval gene sequences from congenic resistant C3H.PRI-Flv(r) and susceptible C3H/He mouse strains identified the 2'-5'-oligoadenylate synthetase 1b (Oas1b) gene as Flv. However, since these mouse strains differ by a 31 cM region, the possible involvement of differences in other linked genes in the resistant phenotype could not be absolutely ruled out. Knock-in of the Oas1b resistance allele into a susceptible mouse strain produced mice with the flavivirus resistance phenotype, confirming that this phenotype is mediated by a single gene.
Scherbik
Svetlana V
SV
Department of Biology, Georgia State University, P.O. Box 4010, Atlanta, GA 30302-4010, USA.
Kluetzman
Kerri
K
Perelygin
Andrey A
AA
Brinton
Margo A
MA
D015088:2',5'-Oligoadenylate Synthetase / Q000737:chemistry / Q000235:genetics*; D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D004198:Disease Susceptibility; D005416:Flavivirus / Q000472:pathogenicity; D018177:Flavivirus Infections / Q000235:genetics* / Q000276:immunology*; D020022:Genetic Predisposition to Disease; D007113:Immunity, Innate; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010641:Phenotype; D011995:Recombination, Genetic; D013045:Species Specificity
D015088:2',5'-Oligoadenylate Synthetase