Effects of resveratrol, a grape polyphenol, on catecholamine secretion and synthesis in cultured bovine adrenal medullary cells.
We report the effects of resveratrol, a polyphenol found in the skins of red grapes, on catecholamine secretion and synthesis in cultured bovine adrenal medullary cells. Resveratrol suppressed catecholamine secretion and (22)Na(+) and (45)Ca(2+) influx induced by acetylcholine, an agonist of nicotinic acetylcholine receptors, in a concentration-dependent manner (IC(50)=20.4, 11.0, and 62.8 microM, respectively). Resveratrol also inhibited catecholamine secretion induced by veratridine, an activator of voltage-dependent Na(+) channels, and 56 mM K(+), an activator of voltage-dependent Ca(2+) channels, at concentrations similar to those for (45)Ca(2+) influx. Resveratrol directly inhibited the current evoked by acetylcholine in Xenopus oocytes expressing alpha3beta4 neuronal nicotinic acetylcholine receptors (IC(50)=25.9 microM). Furthermore, resveratrol (IC(50)=5.32 microM) attenuated (14)C-catecholamine synthesis induced by acetylcholine. The present findings suggest that resveratrol inhibits acetylcholine-induced catecholamine secretion and synthesis through suppressing ion influx in cultured bovine adrenal medullary cells.
Shinohara
Yuko
Y
Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1, Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
Toyohira
Yumiko
Y
Ueno
Susumu
S
Liu
Minhui
M
Tsutsui
Masato
M
Yanagihara
Nobuyuki
N
D000109:Acetylcholine / Q000494:pharmacology; D000313:Adrenal Medulla / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002395:Catecholamines / Q000096:biosynthesis / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D006632:Histamine / Q000494:pharmacology; D017136:Ion Transport / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols; D011188:Potassium / Q000494:pharmacology; D011978:Receptors, Nicotinic / Q000235:genetics / Q000502:physiology; D000077185:Resveratrol; D012964:Sodium / Q000378:metabolism; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology*; D014443:Tyrosine / Q000378:metabolism; D014701:Veratridine / Q000494:pharmacology; D027843:Vitis / Q000737:chemistry*; D014981:Xenopus; D020867:omega-Agatoxin IVA / Q000494:pharmacology; D020866:omega-Conotoxin GVIA / Q000494:pharmacology
D000975:Antioxidants; D002395:Catecholamines; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D011978:Receptors, Nicotinic; D013267:Stilbenes; C403530:nicotinic receptor alpha3beta4; D020867:omega-Agatoxin IVA; D014443:Tyrosine; D014701:Veratridine; D006632:Histamine; D020866:omega-Conotoxin GVIA; D012964:Sodium; D000109:Acetylcholine; D000077185:Resveratrol; D011188:Potassium; D002118:Calcium