title: Nanoparticle interactions with zinc and iron: implications for toxicology and inflammation.
abstract: BACKGROUND
  Particulate air pollution (PM(10)) consists of a mixture of components, including nanoparticles and metals. Studies from our laboratory have demonstrated that transition metals can potentiate the ability of nanoparticles to induce lung inflammation and that the zinc content of PM(10) was largely responsible for their potential to induce inflammation. These results are also relevant to zinc-containing engineered nanoparticles.
  
  
  OBJECTIVE
  To investigate the potential of ZnCl(2) and FeCl(3) to interact with nanoparticle carbon black in cell-free and biological systems to generate ROS, express pro-inflammatory mediators and cytotoxic ability.
  
  
  METHODS
  ROS production was examined using DCFH-DA. J774 cells were treated for 4 h with 14 nm CB and/or ZnCl(2) before measuring TNF-alpha by ELISA. Cytoskeletal changes were investigated using confocal microscopy. Flow cytometry was used to examine apoptotic/necrotic cells and phagocytic ability.
  
  
  RESULTS
  In a cell-free system the particles generated significant ROS, whereas ZnCl(2) did not. Treatment of cells with 100 microM ZnCl(2), but not FeCl(3), increased TNF-alpha. Treatment with 14 nm CB alone induced TNF-alpha, which was synergistically enhanced by ZnCl(2). No interactions were observed in cells treated with 14 nm CB and FeCl(3). Cytoskeletal changes were observed with increasing concentrations of ZnCl(2). These results were confirmed by flow cytometry indicating that ZnCl(2) induced markers of apoptosis and necrosis. The phagocytic ability of cells was also significantly decreased. Nanoparticle carbon black alone did not induce changes in apoptosis/necrosis or the phagocytosis activity of the cells.
  
  
  CONCLUSIONS
  Despite an inability to induce ROS production, ZnCl(2) stimulated TNF-alpha production which was synergistically enhanced by 14 nm carbon black. The ability of zinc to induce morphological changes and cell death was not altered by nanoparticle treatment.
authors: MRC Human Reproductive Sciences Unit, Centre for Reproductive Biology, 49 Little France, Old Dalkeith Road, Edinburgh EH16 4SB, Scotland, UK.
authors: Martin R
authors: MR
authors: Wilson
authors: Laurent
authors: L
authors: Foucaud
authors: Peter G
authors: PG
authors: Barlow
authors: Gary R
authors: GR
authors: Hutchison
authors: Jill
authors: J
authors: Sales
authors: Richard J
authors: RJ
authors: Simpson
authors: Vicki
authors: V
authors: Stone
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002712:Chlorides / Q000633:toxicity*; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D004357:Drug Synergism; D004797:Enzyme-Linked Immunosorbent Assay; D005290:Ferric Compounds / Q000633:toxicity*; D005434:Flow Cytometry; D007249:Inflammation / Q000139:chemically induced; D018836:Inflammation Mediators / Q000378:metabolism; D008264:Macrophages; D051379:Mice; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000633:toxicity*; D009336:Necrosis / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D053260:Soot / Q000633:toxicity*; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism; D017967:Zinc Compounds / Q000633:toxicity*
chemical_list: D000393:Air Pollutants; D002712:Chlorides; D005290:Ferric Compounds; D018836:Inflammation Mediators; D017382:Reactive Oxygen Species; D053260:Soot; D014409:Tumor Necrosis Factor-alpha; D017967:Zinc Compounds; C016837:zinc chloride; C024555:ferric chloride