title: Use of the TRP1 auxotrophic marker for gene disruption and phenotypic analysis in yeast: a note of warning.
abstract: The TRP1 marker has been commonly used for gene disruption experiments and subsequent phenotypic analysis. However, introduction of the TRP1 gene into a trp1 strain markedly affects growth under many conditions used for phenotypic profiling. Therefore, its use in the past should be revisited and utilization of this marker should be avoided in future analyses.
authors: Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors: Asier
authors: A
authors: González
authors: Carolina
authors: C
authors: Larroy
authors: Josep A
authors: JA
authors: Biosca
authors: Joaquín
authors: J
authors: Ariño
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000235:genetics* / Q000502:physiology; D015415:Biomarkers; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000502:physiology
chemical_list: D015415:Biomarkers; D029701:Saccharomyces cerevisiae Proteins; D019747:Aldose-Ketose Isomerases; C490353:TRP1 protein, S cerevisiae