Retinoic acid enhances Erk phosphorylation in the chick retina.
The transcriptional activator retinoic acid (RA) is a regulator of neural development and regeneration. Synergistic effects with brain-derived neurotrophic factor suggested that RA influences neurotrophin signaling. To test this hypothesis RA was administered systemically to E17 chick embryos, and retinas were prepared 12h and 24h later to measure mRNA or protein expression. While there was no significant influence on activation of Akt, CREB and STAT-3, RA-treatment caused elevated levels of Erk-phosphorylation, a kinase involved in Trk signaling. A small but significant increase in the expression of TrkB mRNA and protein was observed but no significant change in TrkA, TrkC and p75 expression.
Kampmann
Eric
E
Institut für Biologie II, RWTH-Aachen, Kopernikusstrasse 16, D-52074 Aachen, Germany.
Mey
Jörg
J
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D004789:Enzyme Activation / Q000187:drug effects / Q000235:genetics; D048049:Extracellular Signal-Regulated MAP Kinases / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics; D010766:Phosphorylation / Q000187:drug effects; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D020813:Receptor, trkB / Q000187:drug effects / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000187:drug effects* / Q000378:metabolism; D012160:Retina / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D014212:Tretinoin / Q000378:metabolism / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D012333:RNA, Messenger; D017475:Receptors, Nerve Growth Factor; D014212:Tretinoin; D020813:Receptor, trkB; D048049:Extracellular Signal-Regulated MAP Kinases