Quantification of Leuconostoc populations in mixed dairy starter cultures using fluorescence in situ hybridization.
OBJECTIVE
Development of a rapid method to identify and quantify Leuconostoc populations in mesophilic starter cultures.


RESULTS
16S rRNA-targeted oligonucleotide probes were used in a whole cell in situ hybridization assay for the identification of the genus Leuconostoc and an undescribed Leuconostoc ribospecies. The probes were fluorescently labelled and used to quantify the Leuconostoc populations in five different mixed starter cultures.


CONCLUSIONS
There was a good correlation between the results obtained using fluorescence in situ hybridization (FISH) with that of standard plate counting methods.


CONCLUSIONS
To develop a FISH method capable of identifying and quantifying the Leuconostoc population in starter cultures within 1 day.
Olsen
K N
KN
Chr. Hansen A/S, Bøge Allé 10-12, Hørsholm, Denmark. kna@kvl.dk
Brockmann
E
E
Molin
S
S
D002611:Cheese / Q000382:microbiology*; D005285:Fermentation; D005516:Food Microbiology; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007934:Leuconostoc / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D015345:Oligonucleotide Probes; D010539:Permeability; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity
D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S