Suction blister fluid as potential body fluid for biomarker proteins.
Early diagnosis is important for effective disease management. Measurement of biomarkers present at the local level of the skin could be advantageous in facilitating the diagnostic process. The analysis of the proteome of suction blister fluid, representative for the interstitial fluid of the skin, is therefore a desirable first step in the search for potential biomarkers involved in biological pathways of particular diseases. Here, we describe a global analysis of the suction blister fluid proteome as potential body fluid for biomarker proteins. The suction blister fluid proteome was compared with a serum proteome analyzed using identical protocols. By using stringent criteria allowing less than 1% false positive identifications, we were able to detect, using identical experimental conditions and amount of starting material, 401 proteins in suction blister fluid and 240 proteins in serum. As a major result of our analysis we construct a prejudiced list of 34 proteins, relatively highly and uniquely detected in suction blister fluid as compared to serum, with established and putative characteristics as biomarkers. We conclude that suction blister fluid might potentially serve as a good alternative biomarker body fluid for diseases that involve the skin.
Kool
Jeroen
J
Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
Reubsaet
Léon
L
Wesseldijk
Feikje
F
Maravilha
Raquel T
RT
Pinkse
Martijn W
MW
D'Santos
Clive S
CS
van Hilten
Jacobus J
JJ
Zijlstra
Freek J
FJ
Heck
Albert J R
AJ
D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D001768:Blister / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism; D001826:Body Fluids / Q000737:chemistry / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D045604:Extracellular Fluid / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006801:Humans; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D013396:Suction
D015415:Biomarkers; D001798:Blood Proteins; D011506:Proteins; D020543:Proteome