title: DNA sequence- and conformation-directed positioning of nucleosomes by chromatin-remodeling complexes.
abstract: Chromatin-remodeling complexes can translocate nucleosomes along the DNA in an ATP-coupled reaction. This process is an important regulator of all DNA-dependent processes because it determines whether certain DNA sequences are found in regions between nucleosomes with increased accessibility for other factors or wrapped around the histone octamer complex. In a comparison of seven different chromatin-remodeling machines (ACF, ISWI, Snf2H, Chd1, Mi-2, Brg1, and NURF), it is demonstrated that these complexes can read out DNA sequence features to establish specific nucleosome-positioning patterns. For one of the remodelers, ACF, we identified a 40-bp DNA sequence element that directs nucleosome positioning. Furthermore, we show that nucleosome positioning by the remodelers ACF and Chd1 is determined by a reduced affinity to the end product of the translocation reaction. The results suggest that the linkage of differential remodeling activities with the intrinsic binding preferences of nucleosomes can result in establishing distinct chromatin structures that depend on the DNA sequence and define the DNA accessibility for other protein factors.
authors: Division of Genome Organization and Function, Deutsches Krebsforschungszentrum and Bioquant, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.
authors: Karsten
authors: K
authors: Rippe
authors: Anna
authors: A
authors: Schrader
authors: Philipp
authors: P
authors: Riede
authors: Ralf
authors: R
authors: Strohner
authors: Elisabeth
authors: E
authors: Lehmann
authors: Gernot
authors: G
authors: Längst
mesh_terms: D000818:Animals; D001483:Base Sequence; D002843:Chromatin / Q000502:physiology* / Q000648:ultrastructure; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D008322:Mammals; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D002843:Chromatin; D009707:Nucleosomes; D004247:DNA