Antioxidant properties of ganglioside micelles.
Antioxidant activity of gangliosides GM1 and GT1b in the Fenton type of reaction was investigated by EPR spectroscopy using DMPO as a spin trap. Hydroxyl radical spin adduct signal intensity was significantly reduced in the presence of gangliosides at their micellar concentrations. Mean micellar hydrodynamic diameter was not changed, whereas significant changes in negative Zeta potential values were observed as evidenced by Zetasizer Nano ZS. This study showed that the primary mode of ganglioside action was not due to direct scavenging of OH., but rather to the inhibition of hydroxyl radical formation. This phenomenon is related to the ability of ganglioside micelles to bind oppositely charged ferrous ions, thus reducing their concentration and consequently inhibiting OH. formation.
Gavella
Mirjana
M
Laboratory of Cell Biochemistry, Vuk Vrhovac University Clinic for Diabetes, Endocrinology and Metabolic Diseases, Zagreb, Croatia. mirjana.gavella@idb.hr
Kveder
Marina
M
Lipovac
Vaskresenija
V
Jurasin
Darija
D
Filipovi-Vincekovic
Nada
N
D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000737:chemistry; D005609:Free Radicals; D005677:G(M1) Ganglioside / Q000737:chemistry*; D005678:G(M2) Ganglioside / Q000737:chemistry*; D005732:Gangliosides / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide; D017665:Hydroxyl Radical / Q000737:chemistry; D007501:Iron; D008823:Micelles; D008954:Models, Biological; D008956:Models, Chemical; D008968:Molecular Conformation; D010710:Phosphates / Q000737:chemistry
D000975:Antioxidants; C045076:Fenton's reagent; D016166:Free Radical Scavengers; D005609:Free Radicals; D005732:Gangliosides; D008823:Micelles; D010710:Phosphates; D005678:G(M2) Ganglioside; D017665:Hydroxyl Radical; D005677:G(M1) Ganglioside; D006861:Hydrogen Peroxide; D007501:Iron