title: Structural characterization of metabolites of salvianolic acid B from Salvia miltiorrhiza in normal and antibiotic-treated rats by liquid chromatography-mass spectrometry.
abstract: This study was conducted to compare the in vivo metabolites of salvianolic acid B (Sal B) between normal rats and antibiotic-treated rats and to clarify the role of intestinal bacteria on the absorption, metabolism and excretion of Sal B. A valid method using LC-MS(n) analysis was established for identification of rat biliary and fecal metabolites. And isolation of normal rat urinary metabolites by repeated column chromatography was applied in this study. Four biliary metabolites and five fecal metabolites in normal rats were identified on the basis of their MS(n) fragmentation patterns. Meanwhile, two normal rat urinary metabolites were firstly identified on the basis of their NMR and MS data. In contrast, no metabolites were detected in antibiotic-treated rat urine and bile, while the prototype of Sal B was found in antibiotic-treated rat feces. The differences of in vivo metabolites between normal rats and antibiotic-treated rats were proposed for the first time. Furthermore, it was indicated that the intestinal bacteria showed an important role on the absorption, metabolism and excretion of Sal B. This investigation provided scientific evidence to infer the active principles responsible for the pharmacological effects of Sal B.
authors: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, No. 38 Xueyuan Road, Beijing 100083, PR China.
authors: Man
authors: M
authors: Xu
authors: Hui
authors: H
authors: Guo
authors: Jian
authors: J
authors: Han
authors: Shi-feng
authors: SF
authors: Sun
authors: Ai-hua
authors: AH
authors: Liu
authors: Bao-rong
authors: BR
authors: Wang
authors: Xiao-chi
authors: XC
authors: Ma
authors: Peng
authors: P
authors: Liu
authors: Xue
authors: X
authors: Qiao
authors: Zi-chuan
authors: ZC
authors: Zhang
authors: De-an
authors: DA
authors: Guo
mesh_terms: D000818:Animals; D001419:Bacteria / Q000187:drug effects / Q000378:metabolism; D001572:Benzofurans / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D009355:Neomycin / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013307:Streptomycin / Q000494:pharmacology; D053630:Depsides
chemical_list: D001572:Benzofurans; D009355:Neomycin; D013307:Streptomycin; C076944:salvianolic acid B; D053630:Depsides