title: Analysis of differently expressed proteins and transcripts in gills of Penaeus vannamei after yellow head virus infection.
abstract: In this proteomic analysis of gills from yellow head virus (YHV)-infected Penaeus vannamei, we identified 13 spots with up-regulated protein expression levels and five spots with down-regulated levels. LC-nanoESI-MS/MS indicated that the up-regulated proteins included enzymes in the glycolytic pathway, the tricarboxylic acid cycle and amino acid metabolism. The other up-regulated proteins were arginine kinase, imaginal disk growth factor (IDGF) and a Ras-like GTP binding protein. By contrast, expression levels were reduced for an SCP-calcium binding protein (SCP), actin-1, a valosin-containing protein, and Rab11. Time-course assays by real time RT-PCR revealed no significant increase in mRNA level of glycolytic enzymes and arginine kinase. However, a significant decrease in SCP mRNA was observed. The present results are consistent with previously published work and suggest that a decrease in SCP expression may play an important role in the shrimp response to viral infections in general.
authors: Center of Excellence for Shrimp Molecular Biology and Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors: Triwit
authors: T
authors: Rattanarojpong
authors: Hao-Ching
authors: HC
authors: Wang
authors: Chu-Fang
authors: CF
authors: Lo
authors: Timothy W
authors: TW
authors: Flegel
mesh_terms: D000818:Animals; D005880:Gills / Q000378:metabolism* / Q000821:virology; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D020543:Proteome / Q000096:biosynthesis* / Q000235:genetics; D012327:RNA Virus Infections / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D046828:Roniviridae / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D020543:Proteome; D012367:RNA, Viral