Proteins at membrane surfaces-a review of approaches.
Membrane proteins are critical for normal cellular differentiation and function, and alterations in these proteins often leads to cell dysfunction and disease. Membrane proteomics aims to identify the membrane protein constituents, their posttranslational modifications, protein-protein interactions, and dynamics. Efforts to identify membrane proteins and elucidate their dynamics have been plagued by the challenges presented by studying water insoluble proteins that are distributed among a range of membranes in a cell and often occur at a relatively low abundance. This brief review presents a summary of the literature related to membrane proteomics with an emphasis on efforts to develop effective protocols for the enrichment of membrane proteins, particularly those located in the plasma membrane.
Macher
Bruce A
BA
Department of Chemistry and Biochemistry, 1600 Holloway Ave., San Francisco State University, San Francisco, CA 94132, USA. macher@sfsu.edu
Yen
Ten-Yang
TY
D000595:Amino Acid Sequence; D001710:Biotin; D002846:Chromatography, Affinity; D003902:Detergents; D015180:Electrophoresis, Gel, Two-Dimensional; D037102:Lectins; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry; D040901:Proteomics; D012995:Solubility; D012997:Solvents; D053719:Tandem Mass Spectrometry
D003902:Detergents; D037102:Lectins; D008565:Membrane Proteins; D011134:Polysaccharides; D012997:Solvents; D001710:Biotin