Evaluation of genes from eIF4E and eIF4G multigenic families as potential candidates for partial resistance QTLs to Rice yellow mottle virus in rice.
QTLs for partial resistance to Rice yellow mottle virus (RYMV) in rice were mapped in two populations of doubled-haploid lines (DHLs) and recombinant inbred lines (RILs) derived from the same cross but evaluated for different resistance criteria (virus content and symptom severity). An integrative map was used to compare the two genetic maps and a global analysis of both populations was performed. Most of the QTLs previously identified in DHL population were confirmed with increased significance and precision. As many recent studies evidenced the role of eukaryotic translation initiation factors (eIF) of 4E and 4G families in plant susceptibility to RNA viruses, we checked if these genes co-locate with QTLs of resistance to RYMV. Their systematic in silico identification was carried out on the rice genome and their physical locations were compared to QTL positions on the integrative map. In order to confirm or not the co-locations observed, the analysis was completed by evaluation of near-isogenic lines, QTL fine mapping and sequencing of candidate genes. Three members from eIF4G family could be retained as reliable candidates whereas eIF4E genes, commonly found to govern resistances in other plant/virus interactions, were discarded. Together with the recent identification of an eIF(iso)4G as a major resistance gene, data suggests an important role of genes from eIF4G family in rice resistance to RYMV but does not exclude the contribution of factors different from the translation initiation complex.
Boisnard
Arnaud
A
UMR 5096, Laboratoire Génome et Développement des Plantes, IRD/CNRS/Université de Perpignan, BP 64501, 34394, Montpellier Cedex 5, France.
Albar
Laurence
L
Thiéméle
Deless
D
Rondeau
Myriam
M
Ghesquière
Alain
A
D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D018744:DNA, Plant; D039561:Eukaryotic Initiation Factor-4E / Q000235:genetics*; D039603:Eukaryotic Initiation Factor-4G / Q000235:genetics*; D018745:Genome, Plant; D005810:Multigene Family; D012275:Oryza / Q000235:genetics* / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D010942:Plant Viruses / Q000472:pathogenicity*; D040641:Quantitative Trait Loci
D018744:DNA, Plant; D039561:Eukaryotic Initiation Factor-4E; D039603:Eukaryotic Initiation Factor-4G