High-performance proteomics as a tool in biomarker discovery.
Biomarkers allowing early detection of disease or therapy control have a huge influence in curing a disease. A wide variety of methods were applied to find new biomarkers. In contrast to methods focused on DNA or mRNA techniques, approaches considering proteins as potential biomarker candidates have the advantage that proteins are more diverse than DNA or RNA and are more reflective of a biological system. Here, we present an approach for the identification of new biomarkers relying on our experience from the past 10 years of proteomics, outlining a concept of "high-performance proteomics" This approach is based on quantitative proteome analysis using a sufficient number of clinical samples and statistical validation of proteomics data by independent methods, such as Western blot analysis or immunohistochemistry.
Meyer
Helmut E
HE
Medizinisches Proteom-Center, Ruhr-University Bochum, Bochum, Germany. helmut.e.meyer@rub.de
Stühler
Kai
K
D015415:Biomarkers / Q000032:analysis* / Q000097:blood; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D010190:Pancreatic Neoplasms / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012107:Research Design; D014018:Tissue Distribution
D015415:Biomarkers