Formation of dentin-like particles in dentin defects above exposed pulp by controlled release of fibroblast growth factor 2 from gelatin hydrogels.
The induction of dentin formation on exposed dental pulp is a major challenge in research on the regeneration of the dentin-pulp complex. We examined the effects of fibroblast growth factor 2 (FGF2), which was delivered in either a collagen sponge (noncontrolled release) or incorporated into gelatin hydrogels (controlled release), on the formation of dentin in exposed rat molar pulps. During the early phase of pulp wound healing, pulp cell proliferation and invasion of vessels into dentin defects above exposed pulp were induced in both groups. In the late phase, the induction of dentin formation was distinctly different between the 2 types of FGF2 release. The noncontrolled release of free FGF2 from collagen sponge induced excessive reparative dentin formation in the residual dental pulp, although dentin defects were not noted. In contrast, controlled release of FGF2 from gelatin hydrogels induced the formation of dentin-like particles with dentin defects above exposed pulp. These results suggest the possibility of a novel therapeutic approach for dentin-pulp complex by controlled release of bioactive FGF2.
Kikuchi
Naoki
N
Division of Pulp Biology, Operative Dentistry, and Endodontics, Department of Cariology and Periodontology, Science of Oral Functions, Kyushu Dental College, Kitakyushu, Japan.
Kitamura
Chiaki
C
Morotomi
Takahiko
T
Inuyama
Yoshio
Y
Ishimatsu
Hirotaka
H
Tabata
Yashuhiko
Y
Nishihara
Tatsuji
T
Terashita
Masamichi
M
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003692:Delayed-Action Preparations; D003782:Dental Pulp / Q000187:drug effects / Q000473:pathology; D003789:Dental Pulp Exposure / Q000188:drug therapy* / Q000473:pathology; D003804:Dentin / Q000187:drug effects* / Q000473:pathology; D003809:Dentin, Secondary / Q000187:drug effects / Q000473:pathology; D003810:Dentinogenesis / Q000187:drug effects* / Q000502:physiology; D004337:Drug Carriers; D016326:Extracellular Matrix Proteins; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000627:therapeutic use*; D005780:Gelatin; D005781:Gelatin Sponge, Absorbable; D020100:Hydrogels; D008963:Molar; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010750:Phosphoproteins / Q000032:analysis; D011498:Protein Precursors / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012795:Sialoglycoproteins / Q000032:analysis; D013047:Specific Pathogen-Free Organisms; D014074:Tooth Calcification / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects; D000099311:Dentin Sialophosphoprotein
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D016326:Extracellular Matrix Proteins; D016222:Fibroblast Growth Factor 2; D005780:Gelatin; D020100:Hydrogels; D010750:Phosphoproteins; D011498:Protein Precursors; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein