title: Polypeptide from Chlamys farreri attenuates murine thymocytes damage induced by ultraviolet B.
abstract: OBJECTIVE
  Polypeptide from Chlamys farreri (PCF, molecular mass is 879) is a new marine polypeptide compound isolated from Chlamys farreri. This study investigates the possible protective roles and the mechanism of PCF against ultraviolet B (UVB)-induced apoptosis in murine thymocytes.
  
  
  METHODS
  The rate of apoptosis and caspase-3 activation was measured by flow cytometry. The expression of stress-response genes c-fos and c-jun was observed by RT-PCR. Western blot analysis was performed to determine the release of cytochrome c.
  
  
  RESULTS
  It was found that UVB induced murine thymocyte death. The cells treated with UVB showed an increase in cytochrome c release, caspase-3 activity, as well as in the expression of c-fos and c-jun. In addition, all were involved in UVB-induced cell apoptosis.
  
  
  CONCLUSIONS
  Our present observations pointed to the ability of PCF to avert UVB-induced apoptosis in thymocytes by modulating c-fos and c-jun expression, cytochrome c release, and the consequent activation of caspase-3, which were essential components of the UV-induced cell apoptotic pathway. The results suggested that PCF is a promising protective substance against UV radiation.
authors: Department of Pharmacochemistry, Ocean University of China, Qingdao 266003, China.
authors: Hai-Ying
authors: HY
authors: Chen
authors: Xiao
authors: X
authors: Chu
authors: Chun-Ling
authors: CL
authors: Yan
authors: Xue-Hong
authors: XH
authors: Chen
authors: Mi
authors: M
authors: Sun
authors: Yue-Jun
authors: YJ
authors: Wang
authors: Chun-Bo
authors: CB
authors: Wang
authors: Wen-Gong
authors: WG
authors: Yu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D045304:Cytochromes c / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D016762:Genes, fos / Q000235:genetics; D016758:Genes, jun / Q000235:genetics; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D049897:Pectinidae / Q000737:chemistry*; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D013950:Thymus Gland / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D008419:Materia Medica; D010455:Peptides; D045304:Cytochromes c; D053148:Caspase 3