title: Reduction of serum antioxidative capacity during hemodialysis.
abstract: BACKGROUND
  Hemodialysis (HD) is thought to exacerbate oxidative stress (OS). The purpose of this study was to assess patients' oxidative status during and after HD, and to clarify the relation between oxidative status and antioxidant solutes such as uric acid (UA) and ascorbic acid (AsA).
  
  
  METHODS
  Serum diacron-reactive oxygen metabolites (d-ROM), and biological antioxidant potential (BAP), as well as serum concentrations of UA, AsA, urea nitrogen (UN), and creatinine (Cre) were measured in eight HD patients at six time points during and after HD. Inflow and outflow dialyzer blood was evaluated during HD or sham HD (no dialysate flow). Further, BAP was evaluated by adding UA or AsA to post-HD serum.
  
  
  RESULTS
  No changes were found in d-ROM over time, whereas BAP was significantly decreased by HD. No differences were found in d-ROM during HD or sham HD, or between inflow and outflow blood. During HD, significantly lower BAP levels were found in outflow blood than in inflow blood. Serum UA and AsA levels were decreased by HD; however, no increases were observed in BAP levels after the addition of these molecules to post-HD serum.
  
  
  CONCLUSIONS
  These data indicate that HD reduces serum antioxidative capacity, and the loss of dialyzable molecules may be involved. However, this reduction in capacity does not seem to be caused by the loss of UA or AsA.
authors: Research Division of Dialysis and Chronic Kidney Disease, Tohoku University Graduate School of Medicine, 1-1 Seiryo machi aoba-ku, Sendai, 980-8574, Japan. nkeisuke@ac.cyberhome.ne.jp.
authors: Keisuke
authors: K
authors: Nakayama
authors: Research Division of Dialysis and Chronic Kidney Disease, Tohoku University Graduate School of Medicine, 1-1 Seiryo machi aoba-ku, Sendai, 980-8574, Japan.
authors: Hiroyuki
authors: H
authors: Terawaki
authors: Research Division of Dialysis and Chronic Kidney Disease, Tohoku University Graduate School of Medicine, 1-1 Seiryo machi aoba-ku, Sendai, 980-8574, Japan.
authors: Masaaki
authors: M
authors: Nakayama
authors: Department of Blood Purification, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors: Masashi
authors: M
authors: Iwabuchi
authors: Department of Blood Purification, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors: Toshinobu
authors: T
authors: Sato
authors: Research Division of Dialysis and Chronic Kidney Disease, Tohoku University Graduate School of Medicine, 1-1 Seiryo machi aoba-ku, Sendai, 980-8574, Japan.
authors: Sadayoshi
authors: S
authors: Ito
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000097:blood*; D018592:Cross-Over Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D006435:Renal Dialysis / Q000009:adverse effects*; D014527:Uric Acid / Q000097:blood*
chemical_list: D000975:Antioxidants; D014527:Uric Acid; D001205:Ascorbic Acid