title: Characterization of atypical lymphocytes and immunophenotypes of lymphocytes in patients with dengue virus infection.
abstract: To characterize the immunophenotypes of lymphocytes in patients with dengue infection, we performed flow cytometric analysis of peripheral blood mononuclear cells collected from 49 dengue hemorrhagic fever (DHF), 25 dengue fever (DF), and 26 dengue-like syndrome (DLS) cases. The mean total atypical lymphocytes in DHF (916.1 +/- 685.6 cells/microl) and DF (876.2 +/- 801.9 cells/microl) were higher than those of DLS (310.5 +/- 181.4 cells/microl). An atypical lymphocyte count of 10% or higher was a good indicator of dengue infection (sensitivity 50% and specificity 86%). Flow cytometric studies showed that the percentages of atypical lymphocytes correlated with those of CD19+ B lymphocytes and inversely correlated with the percentages of CD69+ lymphocytes. The mean absolute counts of atypical lymphocytes and CD19+ cells on the discharge day were significantly higher than those on the admission day. Low percentages of TdT+ cells were found in all groups of patients. We concluded that atypical lymphocyte and CD19+ cell counts may be a useful diagnostic tool for dengue infection and the recovery from the disease could be judged when numbers of both cell types are significantly elevated.
authors: Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. tmwus@mahidol.ac.th
authors: Wipawee
authors: W
authors: Jampangern
authors: Kevalin
authors: K
authors: Vongthoung
authors: Akanitt
authors: A
authors: Jittmittraphap
authors: Surapon
authors: S
authors: Worapongpaiboon
authors: Kriengsak
authors: K
authors: Limkittikul
authors: Ampaiwan
authors: A
authors: Chuansumrit
authors: Uraiwan
authors: U
authors: Tarunotai
authors: Manas
authors: M
authors: Chongsa-nguan
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD; D002648:Child; D002675:Child, Preschool; D003715:Dengue / Q000276:immunology*; D003716:Dengue Virus; D006801:Humans; D016130:Immunophenotyping; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D019595:Severe Dengue / Q000175:diagnosis / Q000276:immunology* / Q000821:virology
chemical_list: D015703:Antigens, CD