1-Phenylethynylpyrene (1-PEPy) as refined excimer forming alternative to pyrene: case of DNA major groove excimer.
1-Phenylethynylpyrene fluorochrome was studied as meta- and para-derivatives of arabino-uridine-2'-carbamates in ss and dsDNA. 1-PEPy showed red-shifted emission and increased fluorescence quantum yield compared to pyrene. Although 1-PEPy has very short excited lifetime (<2.5 ns), it is able to form inter- and intrastrand excimers on DNA, probably resulting from spatial preorganization of two dye molecules.
Astakhova
Irina V
IV
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997, Moscow, Russia.
Malakhov
Andrei D
AD
Stepanova
Irina A
IA
Ustinov
Alexey V
AV
Bondarev
Stanislav L
SL
Paramonov
Alexander S
AS
Korshun
Vladimir A
VA
D002241:Carbohydrates / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009025:Morpholines; D009691:Nucleic Acid Denaturation; D009705:Nucleosides / Q000737:chemistry; D010777:Photochemistry; D011721:Pyrenes / Q000737:chemistry*; D013696:Temperature
C525966:1-phenylethynylpyrene; D002241:Carbohydrates; D005456:Fluorescent Dyes; D009025:Morpholines; D009705:Nucleosides; D011721:Pyrenes; D004247:DNA; C038974:amorolfine