title: Phylogenetic comparison of the carboxy-terminal region of glycoprotein C (gC) of bovine herpesviruses (BoHV) 1.1, 1.2 and 5 from South America (SA).
abstract: Different types and subtypes of bovine herpesvirus 1 and 5 (BoHV-1 and BoHV-5) have been associated to different clinical conditions of cattle, in such a way that type/subtype differentiation has become an essential tool for understanding the pathogenesis and epidemiology of BoHV infections. In search for a genomic region that would allow a clear distinction between BoHV-1 and BoHV-5, the carboxy-terminal portion of glycoprotein C (gC), corresponding to residues 321-450 (BoHV-1) and 301-429 (BoHV-5) of 23 South American (SA) isolates (Brazil mostly) was amplified and sequenced. The nucleotide sequence alignments revealed levels of genomic similarity ranging from 98.7 to 99.8% among BoHV-1 isolates, 88.3 to 92% between BoHV-1/BoHV-5 and 96 to 99.7% among BoHV-5 isolates. At the amino acid level, sequence similarity varied ranging from 97.5 to 99.5% among BoHV-1, 77.5 to 84.4% between BoHV-1/BoHV-5 and 92.1 to 99.5% (BoHV-5/BoHV-5). The isolates could be clearly separated into BoHV-1.1, BoHV-1.2 and BoHV-5 after phylogenetic analysis. The results suggest that the phylogenetic analysis performed here can be used as a potential molecular epidemiological tool for herpesviruses.
authors: Embrapa Suínos e Aves, BR 153, Km 110, CEP: 89700-000, Concórdia, SC, Brazil. pesteves@cnpsa.embrapa.br
authors: P A
authors: PA
authors: Esteves
authors: O A
authors: OA
authors: Dellagostin
authors: L S
authors: LS
authors: Pinto
authors: A D
authors: AD
authors: Silva
authors: F R
authors: FR
authors: Spilki
authors: J R
authors: JR
authors: Ciacci-Zanella
authors: S O
authors: SO
authors: Hübner
authors: R
authors: R
authors: Puentes
authors: J
authors: J
authors: Maisonnave
authors: A C
authors: AC
authors: Franco
authors: F A M
authors: FA
authors: Rijsewijk
authors: H B C R
authors: HB
authors: Batista
authors: T F
authors: TF
authors: Teixeira
authors: D
authors: D
authors: Dezen
authors: A P
authors: AP
authors: Oliveira
authors: C
authors: C
authors: David
authors: C W
authors: CW
authors: Arns
authors: P M
authors: PM
authors: Roehe
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000821:virology*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D025881:Herpesvirus 5, Bovine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D013020:South America / Q000453:epidemiology; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1