[Mosaic expression pattern of the nptII gene in transgenic tobacco plants Nu 21].
Mosaic expression pattern of the nptII gene in transgenic tobacco Nu 21 leaf somatic cells was demonstrated. Inheritance of this phenotype (in T1-T4 and F1 backcrosses) was revealed. Three plant groups were distinguished, with low frequency of variegation manifestation (0-21.8%), with the high frequency of mosaic progeny (63.1 to 100%), and the intermediate type, where the frequency of the appearance of mosaic plants varied in a wide range, from 0 to 100%. The data obtained suggested the existence of two metastable states of a transgene in the leaf disk somatic cells (active and silenced), which could be associated with DNA modification, i.e., methylation of cytosine within the nptII gene sequence.
Marenkova-Novoselia
T V
TV
Deĭneko
E V
EV
Shumnyĭ
V K
VK
D003596:Cytosine / Q000378:metabolism; D019175:DNA Methylation; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D009030:Mosaicism; D010641:Phenotype; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes
D010940:Plant Proteins; D003596:Cytosine