Genetic polymorphism in collagen type 1 alpha 2 intron 33 can be used for the initial screening of East Asians quickly and easily by a heteroduplex analysis.
To save time and simplify the matching genetic information of bereaved family with the collected samples for identification of individuals in a mass-scale disaster and terrorism using molecular biological methods, we propose a method to initially screen samples according to different populations. We found a unique deletion (26822-3) in an allele that 62.0% of East Asian individuals had in intron 33 of type I collagen alpha 2 (COL1A2), while most Europeans and Africans did not have it. By making a heteroduplex complex, a visual discrimination was possible using 10% PAGE. A PCR and heteroduplex analysis could identify genetic polymorphism of COL1A2 intron 33 both clearly and accurately, even from decomposed samples or very small amounts of samples within a very short time period (approximately within 150min). As a result, genetic polymorphism of COL1A2 intron 33 is thus considered to be useful to quickly and easily screen East Asians from vast number of samples using a heteroduplex analysis. Consequently, we consider that an initial screening method for East Asians which use a heteroduplex analysis of genetic polymorphism of COL1A2 intron 33 can smoothly and effectively perform various identifications among East Asians when supplies are logistically poor after a mass-scale disaster or terrorism.
Masuda
Tomoo
T
Department of Forensic Medicine, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan. mappu@ndmc.ac.jp
Takada
Yuzo
Y
Murayama
Takako
T
Tokutomi
Tomoaki
T
Kumaki
Fumiyuki
F
Mukaida
Masahiro
M
D003094:Collagen / Q000235:genetics*; D024042:Collagen Type I; D005202:Asia, Eastern / Q000208:ethnology; D018732:Forensic Anthropology / Q000379:methods; D005820:Genetic Testing / Q000379:methods*; D020180:Heteroduplex Analysis; D006801:Humans; D007438:Introns / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*
D024042:Collagen Type I; D003094:Collagen