title: Micro pumping with cardiomyocyte-polymer hybrid.
abstract: This paper presents a hybrid micropump actuated by the up-down motion of a dome shaped cell-polymer membrane composite. The contractile force induced from self-beating cardiomyocytes cultured on the membrane causes shrinkage and relaxation of a microchamber, leading to a flow in a microchannel. Flow direction is controlled by the geometry of diffuser/nozzle in the microchannel. The fabrication process is noninvasive to cells, thus, cardiomyocytes can robustly maintain their activity for a long time. The fluid motion in the microchannel was monitored by tracking 2 microm polystyrene beads. A net flow rate of 0.226 nl min(-1) was obtained in our microscale device. Our device demonstrates a unique performance of a cell-microdevice hybrid lab-on-a-chip that does not require any external power source, preventing electrical or heat shock to analytes.
authors: Biomedical Engineering, Johns Hopkins University, 207 Clark Hall, 3400 N. Charles St., Baltimore, MD 21218, USA. sortpark@gmail.com
authors: Jungyul
authors: J
authors: Park
authors: Il Chaek
authors: IC
authors: Kim
authors: Jeongeun
authors: J
authors: Baek
authors: Misun
authors: M
authors: Cha
authors: Jinseok
authors: J
authors: Kim
authors: Sukho
authors: S
authors: Park
authors: Junghoon
authors: J
authors: Lee
authors: Byungkyu
authors: B
authors: Kim
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D032701:Biomimetics / Q000295:instrumentation* / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D009200:Myocardial Contraction / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology*; D011108:Polymers / Q000737:chemistry*; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012828:Silicones / Q000737:chemistry*; D013314:Stress, Mechanical
chemical_list: D004129:Dimethylpolysiloxanes; D011108:Polymers; D012828:Silicones; C013830:baysilon