Synthetic osteopontin-derived peptide SVVYGLR can induce neovascularization in artificial bone marrow scaffold biomaterials.
We have previously reported that an osteopontin-derived SVVYGLR peptide exhibited potent angiogenic activity in vitro and in vivo. In the present study, the focus points were on the in vitro effect of SVVYGLR on bone marrow stromal cell proliferation, as well as its in vivo effect on bone tissue formation when grafts made of CO3Ap-collagen sponge- as a scaffold biomaterial containing the SVVYGLR motif - were implanted. SVVYGLR peptide promoted bone marrow stromal cell proliferation. When a CO3Ap-collagen sponge containing SVVYGLR peptide was implanted as a graft into a tissue defect created in rat tibia, the migration of numerous vascular endothelial cells - as well as prominent angiogenesis - inside the graft could be detected after one week. These results thus suggested that our scaffold biomaterials including the peptide could be useful for bone tissue regeneration.
Hamada
Yoshinosuke
Y
Department of Molecular Pathology, Osaka University Graduate School of Medicine and Health Science, Osaka 565-0871, Japan. y-hamada@dent.osaka-u.ac.jp
Egusa
Hiroshi
H
Kaneda
Yoshitosi
Y
Hirata
Isao
I
Kawaguchi
Naomasa
N
Hirao
Takafumi
T
Matsumoto
Takuya
T
Yao
Makiko
M
Daito
Kiyoshi
K
Suzuki
Mayuka
M
Yatani
Hirofumi
H
Daito
Michiharu
M
Okazaki
Masayuki
M
Matsuura
Nariaki
N
D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D000818:Animals; D001853:Bone Marrow / Q000098:blood supply / Q000187:drug effects*; D001861:Bone Regeneration / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D053495:Osteopontin / Q000737:chemistry*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D017154:Stromal Cells / Q000187:drug effects
D043925:Angiogenesis Inducing Agents; D010446:Peptide Fragments; D053495:Osteopontin