title: Critical view on the monochlorodimedone assay utilized to detect haloperoxidase activity.
abstract: The current study aimed to identify the halogenating enzymes involved in the biosynthesis of the ambigols A, B, C and tjipanazole D, isolated from the cyanobacterium Fischerella ambigua. Haloperoxidase (HPO) activity within F. ambigua was therefore assayed spectrophotometrically by using monochlorodimedone (MCD) during protein purification. This strategy revealed the isolation of a protein positive in the MCD-assay, but an involvement in halogenating processes could not be verified. N-terminal sequencing rather demonstrated homology to cytochrome c(6) from other cyanobacteria and green algae. From our findings it thus has to be concluded that the spectrophotometrical MCD-assay routinely used to detect HPO activity may yield false positive results, mainly since the assay focuses on the decline of the educt and not on the formation of the product. Our data indicate that the reaction of MCD with proteins of the cytochrome c- family leads to unspecific products.
authors: Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, D-53115 Bonn, Germany.
authors: Claudia
authors: C
authors: Wagner
authors: Ilka M
authors: IM
authors: Molitor
authors: Gabriele M
authors: GM
authors: König
mesh_terms: D000458:Cyanobacteria / Q000201:enzymology; D003512:Cyclohexanones / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010544:Peroxidases / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D003512:Cyclohexanones; C006991:chlorodimedone; D010544:Peroxidases