Light-induced immobilisation of biomolecules as an attractive alternative to microdroplet dispensing-based arraying technologies.
The present work shows how UV 'light-induced molecular immobilisation' (LIMI) of biomolecules onto thiol reactive surfaces can be used to make biosensors, without the need for traditional microdispensing technologies. Using 'LIMI,' arrays of biomolecules can be created with a high degree of reproducibility. This technology can be used to circumvent the need for often expensive nano/microdispensing technologies. The ultimate size of the immobilised spots is defined by the focal area of the UV beam, which for a diffraction-limited beam can be less than 1 microm in diameter. LIMI has the added benefit that the immobilised molecules will be spatially oriented and covalently bound to the surface. The activity of the sensor molecules is retained. Antibody sensor arrays made using LIMI demonstrated successful antigen binding. In addition, the pattern of immobilised molecules on the surface is not restricted to conventional array formats. The ultimate consequence of the LIMI is that it is possible to write complex protein patterns using bitmaps at high resolution onto substrates. Thus, LIMI of biomolecules provides a new technological platform for biomolecular immobilisation and the potential for replacing present microdispensing arraying technologies.
Duroux
Meg
M
NanoBiotechnology Group, Department of Physics and Nanotechnology, Aalborg University, Aalborg, Denmark.
Skovsen
Esben
E
Neves-Petersen
Maria Teresa
MT
Duroux
Laurent
L
Gurevich
Leonid
L
Petersen
Steffen B
SB
D015374:Biosensing Techniques; D006801:Humans; D008027:Light; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods; D018625:Microscopy, Atomic Force; D010777:Photochemistry / Q000379:methods*; D011506:Proteins / Q000032:analysis; D013499:Surface Properties
D011506:Proteins