A multiprotein complex that mediates translational enhancement in Drosophila.
Modulating the efficiency of translation plays an important role in a wide variety of cellular processes and is often mediated by trans-acting factors that interact with cis-acting sequences within the mRNA. Here we show that a cis-acting element, the Hsp83 degradation element (HDE), within the 3'-untranslated region of the Drosophila Hsp83 mRNA functions as a translational enhancer. We show that this element is bound by a multiprotein complex, and we identify components using a novel affinity-based method called tandem RNA affinity purification tagging. Three proteins (DDP1, Hrp48, and poly(A)-binding protein) are components of the HDE-binding complex and function in translational enhancement. Our data support a model whereby the HDE is composed of several cis-acting subelements that represent binding sites for trans-acting factors, and the combined action of these trans-acting factors underlies the ability of the HDE to stimulate translation.
Nelson
Meryl R
MR
Department of Biochemistry, University of Toronto, 1 King's College Circle,Toronto, Ontario, Canada.
Luo
Hua
H
Vari
Heli K
HK
Cox
Brian J
BJ
Simmonds
Andrew J
AJ
Krause
Henry M
HM
Lipshitz
Howard D
HD
Smibert
Craig A
CA
D020413:3' Untranslated Regions / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism*; D039102:Poly(A)-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000502:physiology*; D020871:RNA Stability / Q000502:physiology
D020413:3' Untranslated Regions; D004268:DNA-Binding Proteins; C491076:Dp1 protein, Drosophila; D029721:Drosophila Proteins; D006360:Heat-Shock Proteins; D034441:Heterogeneous-Nuclear Ribonucleoproteins; C487427:Hrb27C protein, Drosophila; C089272:Hsp83 protein, Drosophila; D046912:Multiprotein Complexes; D039102:Poly(A)-Binding Proteins