Evaluation of miniY-STR multiplex PCR systems for extended 16 Y-STR loci.
We developed three short amplicon Y-chromosomal short tandem repeat (miniY-STR) polymerase chain reaction multiplex systems for 16 Y-STR loci (DYS441, DYS446, DYS462, DYS481, DYS485, DYS495, DYS505, DYS510, DYS511, DYS549, DYS 575, DYS578, DYS593, DYS618, DYS638, and DYS643), using newly designed primer sets. In an assay of 238 Japanese males using the three miniY-STR systems, amplification product lengths ranged from 91 to 151 bp for all 16 Y-STR loci. We identified 212 different haplotypes among the 238 individuals, finding haplotype diversity and discrimination capacity of 0.9974 and 0.8908, respectively. An assay of degraded DNA samples using the three miniY-STR multiplex systems, including artificially degraded samples and degraded forensic casework samples, proved remarkably effective. In conclusion, analyses of miniY-STR multiplex systems will play an important role in forensic applications involving degraded DNA samples for which genotyping using only commercial kits is ill-suited.
Asamura
H
H
Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano, 390-8621, Japan. asamura@sch.md.shinshu-u.ac.jp
Fujimori
S
S
Ota
M
M
Oki
T
T
Fukushima
H
H
D044466:Asian People / Q000235:genetics; D041322:Chromosomes, Human, Y; D053836:DNA Degradation, Necrotic; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007564:Japan; D008297:Male; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences
D017931:DNA Primers