title: Analysis of the complete hepatitis B virus genome in patients with genotype C chronic hepatitis and hepatocellular carcinoma.
abstract: Hepatitis B virus (HBV) genotype C and the basic core promoter (BCP) mutations were reported to be associated with the development of hepatocellular carcinoma (HCC). In this study the full sequences of HBV genomes were analyzed in order to find the other predictors of HCC development. We determined the full sequences of HBV genomes in 24 genotype C carriers who developed HCC (HCC group) at the beginning of follow-up and at the time of HCC diagnosis, and 20 patients who did not develop HCC (non-HCC group) served as a control. The number of nucleotide and amino acid substitutions in most regions was higher in the HCC group than in the non-HCC group, and the following substitutions and deletions were found more frequently in the HCC group than in the non-HCC group: G1317A and T1341C/A/G in the X promoter region were detected in 13 and six of the HCC cases, four and none of the non-HCC cases, respectively; and pre-S2 deletion was detected in eight HCC and none of the non-HCC cases. Compared with the wild type X promoter, the mutant type X promoters, M1 (G1317A), M2 (T1341C), and M4 (T1341G) showed increases in activity of 2.3, 3.8, and 1.4 times, respectively, in HepG2 cells. Substitutions and deletion of nucleotides of the HBV genome, especially the pre-S2 deletion and G1317A and T1341C/A/G mutations may be useful markers for predicting the development of HCC.
authors: Department of Medicine and Clinical Oncology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-Ward, Chiba City, Chiba 260-8670, Japan.
authors: Kai Yu
authors: KY
authors: Zhang
authors: Fumio
authors: F
authors: Imazeki
authors: Kenichi
authors: K
authors: Fukai
authors: Makoto
authors: M
authors: Arai
authors: Tatsuo
authors: T
authors: Kanda
authors: Rintaro
authors: R
authors: Mikata
authors: Osamu
authors: O
authors: Yokosuka
mesh_terms: D000328:Adult; D019943:Amino Acid Substitution; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000821:virology*; D045744:Cell Line, Tumor; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D005260:Female; D017930:Genes, Reporter; D016679:Genome, Viral; D005838:Genotype; D006515:Hepatitis B virus / Q000235:genetics*; D019698:Hepatitis C, Chronic / Q000235:genetics* / Q000821:virology*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000821:virology*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012367:RNA, Viral / Q000097:blood / Q000235:genetics; D014162:Transfection; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins