title: Impact of portal versus systemic venous drainage on acute rejection of simultaneous pancreas-kidney transplantation in pig.
abstract: BACKGROUND
  The immunological benefits of portal venous drainage (PVD) in pancreas transplantation remain debated clinically. We established simultaneous pancreas-kidney transplantation (SPK) models with portal venous drainage and systemic venous drainage to compare the impact of venous drainage site on acute rejection in pig.
  
  
  METHODS
  Forty-eight nonrelated, first hybrid landrace pigs were divided into PE (portal-enteric drainage) and SE (systemic-enteric drainage) groups. Type I diabetes mellitus was induced by whole pancreatectomy, and right-side nephrectomy was also performed in the recipients. The donor portal vein was anastomosed to superior mesenteric vein of the recipients in PE group or to the inferior hepatic cava vena of the recipients in SE group. Graft tissue specimens were obtained with laparotomy on Day 3 and 7 after transplant, and the severity of acute rejection was scored according to Nakhleh and Banff criteria.
  
  
  RESULTS
  The cold ischemia time, fasting plasma glucose and urine creatinine of the 2 groups had no statistic difference between 2 groups at Day 1, 3, 5, and 7 after transplant (P>0.05). The occurrence of both pancreas and kidney acute rejections in PE group was significant later and slighter than SE group (P<0.05).
  
  
  CONCLUSIONS
  PVD, compared with SVD, could ameliorate and delay acute rejection in pig SPK. It might become a tolerance inducing method of pancreas transplantation, decrease the cost and improve the quality of SPK, if further confirmed by clinical trials.
authors: Department of General Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
authors: Yong
authors: Y
authors: Tang
authors: You Ping
authors: YP
authors: Li
authors: Jian Shui
authors: JS
authors: Li
authors: Zhao Da
authors: ZD
authors: Zhang
authors: Fang Hai
authors: FH
authors: Han
authors: Wei Ming
authors: WM
authors: Hu
authors: Bo Le
authors: BL
authors: Tian
mesh_terms: D000208:Acute Disease; D000818:Animals; D006084:Graft Rejection / Q000276:immunology* / Q000473:pathology; D016030:Kidney Transplantation / Q000276:immunology* / Q000379:methods*; D016035:Pancreas Transplantation / Q000276:immunology* / Q000379:methods*; D013552:Swine; D014680:Veins / Q000502:physiology* / Q000601:surgery*