title: Fmoc-based solid phase chemical synthesis of 71-meric neuregulin 1-beta1, an epidermal growth factor-like domain.
abstract: The human neuregulin 1-beta1 (NRG1-beta1, amino acid residues 176-246) was chemically synthesized by Fmoc-based solid phase peptide synthesis (SPPS) followed by folding in a redox buffer. The biological activity of the synthesized NRG1-beta1 was confirmed by ligand-induced tyrosine phosphorylation on Chinese hamster ovary (CHO) cells expressing ErbB-4.
authors: Department of Medicinal Chemistry, Center for Frontier Research in Medicinal Science, 21st Century COE Program, Kyoto Pharmaceutical University, Yamashina-ku, Kyoto 607-8412, Japan.
authors: Taeko
authors: T
authors: Kakizawa
authors: Shizuyo
authors: S
authors: Koide-Yoshida
authors: Tooru
authors: T
authors: Kimura
authors: Hiromasa
authors: H
authors: Uchimura
authors: Yoshio
authors: Y
authors: Hayashi
authors: Kazuki
authors: K
authors: Saito
authors: Yoshiaki
authors: Y
authors: Kiso
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D004815:Epidermal Growth Factor / Q000737:chemistry* / Q000502:physiology; D005449:Fluorenes / Q000737:chemistry*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D020890:Neuregulin-1; D010455:Peptides / Q000138:chemical synthesis*; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D017434:Protein Structure, Tertiary
chemical_list: D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; C094131:NRG1 protein, human; D009419:Nerve Tissue Proteins; D020890:Neuregulin-1; D010455:Peptides; D011108:Polymers; D004815:Epidermal Growth Factor