title: The role of peptide motifs in the evolution of a protein network.
abstract: Naturally occurring proteins in cellular networks often share peptide motifs. These motifs have been known to play a pivotal role in protein interactions among the components of a network. However, it remains unknown how these motifs have contributed to the evolution of the protein network. Here we addressed this issue by a synthetic biology approach. Through the motif programming method, we have constructed an artificial protein library by mixing four peptide motifs shared among the Bcl-2 family proteins that positively or negatively regulate the apoptosis networks. We found one strong pro-apoptotic protein, d29, and two proteins having moderate, but unambiguous anti-apoptotic functions, a10 and d16, from the 28 tested clones. Thus both the pro- and anti-apoptotic modulators were present in the library, demonstrating that functional proteins with opposing effects can emerge from a single pool prepared from common motifs. Motif programming studies have exhibited that the annotated function of the motifs were significantly influenced by the context that the motifs embedded. The results further revealed that reshuffling of a set of motifs realized the promiscuous state of protein, from which disparate functions could emerge. Our finding suggests that motifs contributed to the plastic evolvability of the protein network.
authors: Department of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Koto-ku, Tokyo, Japan. saito@lif.kyoto-u.ac.jp
authors: Hirohide
authors: H
authors: Saito
authors: Shunnichi
authors: S
authors: Kashida
authors: Tan
authors: T
authors: Inoue
authors: Kiyotaka
authors: K
authors: Shiba
mesh_terms: D020816:Amino Acid Motifs; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D045744:Cell Line, Tumor; D019143:Evolution, Molecular; D015723:Gene Library; D006801:Humans; D010455:Peptides / Q000737:chemistry; D025941:Protein Interaction Mapping; D025521:Tumor Suppressor Proteins / Q000737:chemistry / Q000378:metabolism; D051020:bcl-X Protein / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; C430097:ELAC1 protein, human; D010455:Peptides; D025521:Tumor Suppressor Proteins; D051020:bcl-X Protein