title: [Effect of platelet-rich plasma and latissimus dorsi myofascia with blood vessel on vascularization of tissue engineered bone in dogs].
abstract: OBJECTIVE
  To study the effect of platelet-rich plasma (PRP) and latissimus dorsi myofascia with blood vessel on vascularization of tissue engineered bone in dogs.
  
  
  METHODS
  Bone marrow stromal cells (BMSCs) were isolated from iliac bone of dogs. PRP was obtained from the same dog. And demineralized bone matrix (DBM) were prepared from homologuous bone. ABCD 4 areas were divided on the back of dog. PRP/BMSCs/DBM was implanted around the vessels of lattisimus dorsi muscle in the A. PRP/BMSCs/DBM wrapped by superficial fascia in the B. BMSCs/DBM was implanted around vessels of lattisimus dorsi muscle in the C. BMSCs/DBM wrapped by superficial fascia in the D area of the same dog. 4, 8, 12 weeks after implantation, gross specimen and histology examination were made.
  
  
  RESULTS
  Osteogenesis and blood vessel formation results were A>B>C>D area.
  
  
  CONCLUSIONS
  The results suggested that the PRP and latissimus dorsi myofascia with blood vessels could promote calcification and vascularization in tissue-engineered bone.
authors: Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital of Medical College, College of Stomatology, Qingdao University, Qingdao 266003, China.
authors: Ning-yi
authors: NY
authors: Li
authors: Li-qiang
authors: LQ
authors: Chen
authors: Tao
authors: T
authors: Chen
authors: Xiao-ming
authors: XM
authors: Jin
authors: Rong-tao
authors: RT
authors: Yuan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001842:Bone and Bones; D004285:Dogs; D059630:Mesenchymal Stem Cells; D010012:Osteogenesis; D053657:Platelet-Rich Plasma; D064171:Superficial Back Muscles; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials