Gradual disappearance of vacuolated enterocytes in the small intestine of neonatal piglets.
The unique feature of enterocytes in newborn mammals is the presence of an apical canalicular system (ACS) leading to production of large vacuoles, important for colostral macromolecule uptake. The vacuolated fetal-type enterocytes (VFE) enable transfer of colostral and milk proteins from the intestinal lumen across the epithelium without loosing their biological activity. First VFE are observed in the pig and lamb fetuses in the second trimester of pregnancy, located at the upper part of villi in the proximal region of the fetal small intestine and subsequently in the middle and distal regions. After birth the VFE are replaced with enterocytes lacking ACS. The present study aimed to investigate the depletion of VFE in the small intestine in the sow reared pig neonates during the first postnatal weeks using scanning electron microscopy (SEM). The SEM analysis demonstrated the gradual disappearance of vacuolated enterocytes in time. VFE remained in the jejunum for a few days after birth, whereas in the duodenum single VFE were present only at birth. In the proximal jejunum, the VFE were localized in the upper part of the villi, and disappeared until the day 3 of life. VFE were present in the mid and distal jejunum, and diminished gradually until day 14 of life. By the day 21 of life, the vacuolated cells were not observed neither in the jejunum nor ileum. In conclusion, morphology analysis of pig small intestinal mucosa suggests that replacement of fetal type vacuolated enterocytes is resumed within 21 days after birth.
Skrzypek
T
T
Scanning Electron Microscopy Laboratory, Catholic University of Lublin, Lublin, Poland. tskrzypek@eko.lublin.pl
Valverde Piedra
J L
JL
Skrzypek
H
H
Kazimierczak
W
W
Biernat
M
M
Zabielski
R
R
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D004386:Duodenum / Q000166:cytology / Q000378:metabolism*; D020895:Enterocytes / Q000378:metabolism*; D007082:Ileum / Q000166:cytology / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism; D007583:Jejunum / Q000166:cytology / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D013552:Swine; D013997:Time Factors; D014617:Vacuoles / Q000378:metabolism