title: The influence of the pulsatility of the blood flow on the extent of platelet adhesion.
abstract: A new improved flow system was developed to study the influence of blood flow pulsatility on platelet adhesion on adhesive proteins and bio-medical materials. The pulsatility was introduced by changing the shear rate every 15 s in blood that was aspirated through a perfusion chamber by a syringe pump. The advantage of this new system is that it avoids system related platelet activation. At steady low shear rate (300/s) after 5 min a collagen type III surface was covered for 24.2+/-3.8% with platelets. At steady high shear rate (1300/s) platelet coverage to collagen was 48.8+/-6.8%. When pulsatility was introduced by changing the shear rate was every 15 s form 300/s to 1300/s and vice-versa, platelet coverage after 5 min was increased to 60.4+/-4.0% (p<0.001). After 5 min perfusion samples were taken from the perfusate and the extent of platelet activation was measured. The significant difference in surface expression of P-selectin on platelets is only seen when comparing pulse flow with control (no flow). We concluded that a significant increase in platelet activation during blood pulsatile flow compared with steady flow, which results in an increased platelet adhesion to collagen.
authors: College of Medicine, Shandong University, Jinan, China.
authors: Xiao Min
authors: XM
authors: Zhao
authors: Ya Ping
authors: YP
authors: Wu
authors: Hong Xin
authors: HX
authors: Cai
authors: Ran
authors: R
authors: Wei
authors: Ton
authors: T
authors: Lisman
authors: Ji Ju
authors: JJ
authors: Han
authors: Zuo Li
authors: ZL
authors: Xia
authors: Philip G
authors: PG
authors: de Groot
mesh_terms: D001783:Blood Flow Velocity; D001792:Blood Platelets / Q000737:chemistry / Q000502:physiology*; D024061:Collagen Type III / Q000737:chemistry / Q000378:metabolism; D018056:Hemorheology / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008955:Models, Cardiovascular; D019007:P-Selectin / Q000032:analysis; D010973:Platelet Adhesiveness / Q000502:physiology*; D011673:Pulsatile Flow; D012016:Reference Values; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D024061:Collagen Type III; D019007:P-Selectin