title: SIRT1 transgenic mice show phenotypes resembling calorie restriction.
abstract: We generated mice that overexpress the sirtuin, SIRT1. Transgenic mice have been generated by knocking in SIRT1 cDNA into the beta-actin locus. Mice that are hemizygous for this transgene express normal levels of beta-actin and higher levels of SIRT1 protein in several tissues. Transgenic mice display some phenotypes similar to mice on a calorie-restricted diet: they are leaner than littermate controls; are more metabolically active; display reductions in blood cholesterol, adipokines, insulin and fasted glucose; and are more glucose tolerant. Furthermore, transgenic mice perform better on a rotarod challenge and also show a delay in reproduction. Our findings suggest that increased expression of SIRT1 in mice elicits beneficial phenotypes that may be relevant to human health and longevity.
authors: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors: Laura
authors: L
authors: Bordone
authors: Dena
authors: D
authors: Cohen
authors: Ashley
authors: A
authors: Robinson
authors: Maria Carla
authors: MC
authors: Motta
authors: Ed
authors: E
authors: van Veen
authors: Agnieszka
authors: A
authors: Czopik
authors: Andrew D
authors: AD
authors: Steele
authors: Hayley
authors: H
authors: Crowe
authors: Stephen
authors: S
authors: Marmor
authors: Jianyuan
authors: J
authors: Luo
authors: Wei
authors: W
authors: Gu
authors: Leonard
authors: L
authors: Guarente
mesh_terms: D054392:Adipokines / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D031204:Caloric Restriction; D002784:Cholesterol / Q000097:blood; D007328:Insulin / Q000097:blood; D008136:Longevity / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D010641:Phenotype; D056564:Sirtuin 1; D037761:Sirtuins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D054392:Adipokines; D001786:Blood Glucose; D007328:Insulin; D002784:Cholesterol; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1; D037761:Sirtuins