Loading and unloading of molecular cargo by DNA-conjugated microtubule.
We developed a novel method to load and unload molecular cargos to and from microtubules (MTs) that move on kinesin-coated surfaces. Quantum dots (Qds) (molecular cargo) connected to 21-mer DNA can be selectively loaded on DNA-conjugated MTs through DNA hybridization. The average velocity of the Qd-loaded MTs (0.43 +/- 0.06 microm s(-1) at 25 degrees C) was comparable to that of control MTs. In addition, MTs conjugated with two types DNA sequences can achieve multiloading of Qds. To unload Qd molecular cargos from MTs, the DNA double helix connecting Qds to MTs were cleaved by an appropriate restriction enzyme. This biomolecular motors-based transport system should enable us to construct nanometer-scale devices such as nanobiosensor, nanofluidic system, or nanomachine.
Taira
Shu
S
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan.
Du
Yong-Zhong
YZ
Hiratsuka
Yuich
Y
Uyeda
Taro Q P
TQ
Yumoto
Noboru
N
Kodaka
Masato
M
D004247:DNA / Q000737:chemistry*; D004262:DNA Restriction Enzymes / Q000737:chemistry*; D008870:Microtubules / Q000737:chemistry*; D020409:Molecular Motor Proteins / Q000737:chemistry*; D009038:Motion; D036103:Nanotechnology / Q000379:methods*; D045663:Quantum Dots
D020409:Molecular Motor Proteins; D004247:DNA; D004262:DNA Restriction Enzymes