[Silencing HSV1 gD expression in cultured cells by RNA interference].
To explore the anti-HSV-1 effect of silencing gD gene expression by RNA interference, five 21-nucleotide duplex small interfering RNAs(siRNAs) targeting the HSV1 gD sequence were designed and the gD-EGFP fusion gene expression vector was constructed, then co-transfected into Vero cell, and screened the effective siRNA through analyzing the intensity of the EGFP fluorescence. Finally, the anti-HSV1 effect was confirmed by plaque reduction assay, real-time PCR and daughter virus titration of HSV1 infected Vero cells transfected with siRNAs. The study demonstrated that siRNAs could effectively and specifically inhibit gD gene expression in HSV1-infected cells, but only had a little effect on HSV1 infection, so taking gD as the target of siRNA against HSV1 needs further study.
Zhu
Qin-Chang
QC
Biomedicine Research and Development Center of Jinan University, Guangzhou 510632, China.
Ren
Zhe
Z
Zhang
Chun-Long
CL
Zhang
Mei-Ying
MY
Liao
Hong-Juan
HJ
Liu
Qiu-Ying
QY
Zhang
Pei-Zhuo
PZ
Li
Jiu-Xiang
JX
Hu
Chao-Feng
CF
Wang
Hua-Dong
HD
Wang
Yi-Fei
YF
D000818:Animals; D002522:Chlorocebus aethiops; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism
D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D049452:Green Fluorescent Proteins