Nisin-controlled extracellular production of interleukin-2 in Lactococcus lactis strains, without the requirement for a signal peptide sequence.
Secretion of the cytokine interleukin-2 (IL-2) was investigated in Lactococcus lactis using the secretory machinery of the bacteriocin lactococcin A. Surprisingly, the lcnCD transport genes were not essential for mouse IL-2 secretion. Furthermore, expression of a mature mouse IL-2 gene resulted in interleukin secretion without the requirement for a leader sequence.
Fernández
Antonio
A
Commensals & Microflora, BBSRC Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom. antonio.dumont@bbsrc.ac.uk
Rodríguez
Juan M
JM
Bongaerts
Roy J
RJ
Gasson
Michael J
MJ
Horn
Nikki
N
D000818:Animals; D001430:Bacteriocins / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D007376:Interleukin-2 / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009561:Nisin / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics*
D001430:Bacteriocins; D007376:Interleukin-2; D021382:Protein Sorting Signals; C069325:lactococcin A; D009561:Nisin; D007770:L-Lactate Dehydrogenase