Lack of association between vitamin D receptor genotypes and haplotypes with fat-free mass in postmenopausal Brazilian women.
The relationship between vitamin D receptor (VDR) ApaI, CDX2, BsmI, FokI, and TaqI polymorphisms and fat-free mass (FFM) were examined in 191 postmenopausal Brazilian women (mean age 67.87 +/- 5.22 years). Participants underwent FFM measurements by dual-energy x-ray absorptiometry (DEXA). Whole-blood-extracted genomic DNA was genotyped to the aforementioned polymorphisms and to ancestry-informative markers through minisequencing, using the SNaPshot Multiplex System. Association between VDR polymorphisms and FFM variables was assessed by analysis of covariance. Haplotypes were estimated, and regression-based, haplotype-specific association tests were carried out with the studied phenotypes. No departure from Hardy-Weinberg equilibrium was detected for any polymorphism. None of the investigated VDR allelic variations, individually or analyzed as haplotypes, was associated with FFM phenotypes. The inclusion of individual African genomic ancestry was used as an attempt to correct for population stratification. Further studies in larger sample population are required to confirm these findings.
Moreno Lima
Ricardo
R
Programa de Pós-Graduação em Educação Física da Universidade Católica de Brasília, Brazil.
Silva de Abreu
Breno
B
Gentil
Paulo
P
Cesar de Lima Lins
Tulio
T
Grattapaglia
Dário
D
Pereira
Rinaldo Wellerson
RW
Jacó de Oliveira
Ricardo
R
D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001483:Base Sequence; D001823:Body Composition / Q000235:genetics*; D001938:Brazil; D003430:Cross-Sectional Studies; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008593:Menopause; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D018167:Receptors, Calcitriol / Q000235:genetics*
D018167:Receptors, Calcitriol