Comparison of M-Kyoto solution and histidine-tryptophan-ketoglutarate solution with a trypsin inhibitor for pancreas preservation in islet transplantation.
The use of University of Wisconsin (UW) preservation solution in islet transplantation has some disadvantages, including inhibition of collagenase activity for pancreatic digestion. Histidine-tryptophan-ketoglutarate (HTK) solution has demonstrated an efficacy similar to UW solution for organ preservation in clinical pancreas transplantation. Recently, we reported that islet yield from porcine pancreata was significantly gtreater when they were preserved using M-Kyoto solution compared with UW solution. Here, we compared HTK solution with ulinastatin (M-HTK) and M-Kyoto solution for islet yield. In porcine islet isolation, islet yield after purification was significantly greater in the M-Kyoto/perfluorochemical (PFC) group compared with the M-HTK/PFC group. The M-Kyoto/PFC group had a significantly lower ADP/ATP ratio compared with the M-HTK/PFC group, suggesting that different islet yields might be due to the differences as energy sources of the solutions used. In conclusion, M-Kyoto/PFC solution is better for pancreas preservation before islet isolation than M-HTK/PFC solution.
Noguchi
Hirofumi
H
Transplantation Unit, Kyoto University Hospital, Shogoin,Kyoto, Japan. hirofumn@baylorhealth.edu
Ueda
Michiko
M
Hayashi
Shuji
S
Kobayashi
Naoya
N
Nagata
Hideo
H
Iwanaga
Yasuhiro
Y
Okitsu
Teru
T
Matsumoto
Shinichi
S
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D006639:Histidine; D016381:Islets of Langerhans Transplantation; D007656:Ketoglutaric Acids; D009926:Organ Preservation; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D012996:Solutions; D013552:Swine; D014357:Trypsin; D014361:Trypsin Inhibitors / Q000494:pharmacology*
D007656:Ketoglutaric Acids; D012996:Solutions; D014361:Trypsin Inhibitors; D006639:Histidine; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D014357:Trypsin