title: Effects of a plasmid-encoded qnrA1 determinant in Escherichia coli strains carrying chromosomal mutations in the acrAB efflux pump genes.
abstract: The qnrA1 gene was cloned into a plasmid vector and transformed into Escherichia coli strains carrying chromosomal mutations in the acrAB drug efflux system. In acrAB-deleted strains, and in strains overexpressing acrAB, viable qnrA conferred higher MIC levels (at least a 32-fold increase) for fluoroquinolones than were observed in the parent strains.
authors: Division of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea.
authors: Jin-Yong
authors: JY
authors: Jeong
authors: Eun Sil
authors: ES
authors: Kim
authors: Sang-Ho
authors: SH
authors: Choi
authors: Hyun-Hee
authors: HH
authors: Kwon
authors: Sang-Rok
authors: SR
authors: Lee
authors: Sang-Oh
authors: SO
authors: Lee
authors: Mi-Na
authors: MN
authors: Kim
authors: Jun Hee
authors: JH
authors: Woo
authors: Yang Soo
authors: YS
authors: Kim
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D024841:Fluoroquinolones / Q000494:pharmacology; D017353:Gene Deletion; D018628:Gene Dosage; D008074:Lipoproteins / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics*; D010957:Plasmids
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D024841:Fluoroquinolones; D008074:Lipoproteins; D026901:Membrane Transport Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C083497:AcrA protein, E coli; C083496:AcrB protein, E coli; C485384:Qnr protein, E coli