The C-terminus of the yeast Lsm4p is required for the association to P-bodies.
We previously reported that Saccharomyces cerevisiae mutants in mRNA decapping and mutants expressing a truncated form of the KlLSM4 gene, showed premature senescence and apoptotic phenotypes. Here, we show that this truncated protein is dispersed in the cytoplasm and does not assemble to P-bodies. As reported in decapping mutants, we observed an increase in the number of P-bodies suggesting that the truncation of the protein impairs this process. The number of P-bodies also increases after oxidative stress and is not dependent on the meta-caspase gene YCA1, placing this phenomenon upstream to the onset of apoptosis.
Mazzoni
Cristina
C
Pasteur Institute-Cenci Bolognetti Foundation, Department of Cell and Developmental Biology, University of Rome La Sapienza Piazzale Aldo Moro 5, 00185 Rome, Italy. cristina.mazzoni@uniromal.it
D'Addario
Irene
I
Falcone
Claudio
C
D000595:Amino Acid Sequence; D017209:Apoptosis; D022083:Cytoplasmic Structures / Q000737:chemistry; D005656:Fungal Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D007716:Kluyveromyces / Q000235:genetics; D018384:Oxidative Stress; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000378:metabolism; D012261:Ribonucleoproteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics; D017384:Sequence Deletion
D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D012261:Ribonucleoproteins; D049452:Green Fluorescent Proteins