In vivo genotoxicity of mercury chloride and lead acetate: Micronucleus test on acridine orange stained fish cells.
The genotoxic effects of mercury chloride and lead acetate were evaluated in vivo using the micronucleus (MN) assay on acridine-orange (AO) stained peripheral blood erythrocytes, gill and fin epithelial cells of Carassius auratus auratus. Fish were exposed to three different concentrations of mercury chloride (MC) (1 microg/, 5 microg/L and 10 microg/L) and lead acetate (LA) (10 microg/L, 50 microg/L and 100 microg/L) for 2, 4 and 6 days. A single dose of 5 mg/L cyclophosphamide was used as a positive control. In addition to micronuclei, nuclear buds (NBs) were assessed in the erythrocytes. The ratio of polychromatic and normochromatic erythrocytes (PCE/NCE) in peripheral blood was also evaluated to assess cytotoxicity. MN frequencies in all three tissues were elevated in fish exposed to both LA and MC. However, NBs showed different sensitivity to metal treatments. MN frequencies in both control and treated fish were highest in gill cells and generally lower in erythrocytes and fin cells. PCE/NCE rations decreased in relation to MC and LA treatments. The results of this study indicate that LA and MC have genotoxic and cytotoxic damage in fish and confirmed that AO staining is a suitable technique for in vivo MN test in fish.
Cavaş
Tolga
T
Mersin University, Faculty of Sciences and Letters, Department of Biology, 33342 Mersin, Turkey. tcavas@mersin.edu.tr
D000165:Acridine Orange; D000818:Animals; D001399:Azure Stains; D002478:Cells, Cultured; D004912:Erythrocytes / Q000187:drug effects; D005880:Gills / Q000166:cytology / Q000187:drug effects; D006054:Goldfish / Q000502:physiology*; D008627:Mercuric Chloride / Q000633:toxicity*; D015162:Micronucleus Tests; D008856:Microscopy, Fluorescence; D009153:Mutagens / Q000633:toxicity*; D009942:Organometallic Compounds / Q000633:toxicity*
D001399:Azure Stains; D009153:Mutagens; D009942:Organometallic Compounds; D008627:Mercuric Chloride; D000165:Acridine Orange; C008261:lead acetate