title: Morphologic, ultrastructural, and biochemical identification of apoptosis in vitrified-warmed mouse ovarian tissue.
abstract: OBJECTIVE
  To evaluate the incidence of apoptosis after vitrification warming of mouse ovaries.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  University-based research laboratory.
  
  
  METHODS
  Twelve- to 14-day-old National Medical Research Institute female mice.
  
  
  METHODS
  Vitrification of mouse ovaries using ethylene glycol.
  
  
  METHODS
  Follicle viability assessment by trypan blue testing, morphologic examination by hematoxylin-eosin staining and transmission electron microscopy, apoptosis assessment using the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling method and DNA laddering technique.
  
  
  RESULTS
  No statistically significant difference in follicle viability was observed between vitrified and nonvitrified ovaries. On transmission electron microscopy, vitrified ovaries showed a well-preserved ultrastructure. No sign of apoptosis was observed morphologically or by transferase-mediated deoxyuridine triphosphate nick end-labeling technique in either fresh or vitrified-warmed mouse ovaries. Despite the presence of a laddering pattern of DNA in control induced thymic tissue, no similar pattern was observed in fresh or vitrified-warmed ovaries.
  
  
  CONCLUSIONS
  The data suggest that the vitrification technique does not induce apoptosis in mouse ovarian tissue investigated just after warming.
authors: Department of Anatomy, Tarbiat Modares University, Tehran, Iran.
authors: Tahere
authors: T
authors: Mazoochi
authors: Mojdeh
authors: M
authors: Salehnia
authors: Mojtaba Rezazadeh
authors: MR
authors: Valojerdi
authors: S Javad
authors: SJ
authors: Mowla
mesh_terms: D000818:Animals; D017209:Apoptosis; D002470:Cell Survival; D015925:Cryopreservation; D005260:Female; D051379:Mice; D006080:Ovarian Follicle / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure*