Bactericidal permeability increasing protein gene variants in children with sepsis.
OBJECTIVE
To evaluate the role of genetic polymorphisms of the bactericidal permeability increasing protein (BPI) in pediatric patients with sepsis.


METHODS
Prospective, single-center, case-control study at the pediatric intensive care unit (PICU) of a university hospital.


METHODS
345 consecutive pediatric patients admitted to the PICU with fever, systemic inflammatory response syndrome (SIRS), sepsis, severe sepsis, septic shock, or multiple organ distress syndrome (MODS).


METHODS
DNA was isolated and two BPI gene polymorphisms BPI (G545 > C) Taq and BPI (A645[ > G) 216 were studied in patients and compared with healthy controls.


RESULTS
Genetic analysis of the BPI Taq gene revealed significant differences between healthy controls and the subgroup of febrile patients (p = 0.0243), the subgroup of SIRS and sepsis (p = 0.0101), and the subgroup of severe sepsis, septic shock, and MODS (p = 0.0027), respectively. No statistically significant differences for the BPI 216 gene polymorphism were found between patient and healthy control groups. A statistically significant predisposition to Gram-negative sepsis in patients carrying the BPI Taq GG variant together with the BPI 216 AG or GG variant was revealed (p = 0.0081), and these haplotypes were also associated with death due to sepsis-related complications.


CONCLUSIONS
BPI Taq gene polymorphism is the accurate predictor of the severity of sepsis in children admitted to the PICU.
Michalek
Jaroslav
J
University Hospital Brno, First Department of Pediatrics, Cernopolni 9, 61300 Brno, Czech Republic. jmichalek@fnbrno.cz
Svetlikova
Petra
P
Fedora
Michal
M
Klimovic
Michal
M
Klapacova
Lenka
L
Bartosova
Drahomira
D
Elbl
Lubomir
L
Hrstkova
Hana
H
Hubacek
Jaroslav A
JA
D000293:Adolescent; D000328:Adult; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D001798:Blood Proteins / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D018153:Czech Republic; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000150:complications / Q000235:genetics / Q000401:mortality; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011446:Prospective Studies; D018805:Sepsis / Q000150:complications / Q000235:genetics* / Q000401:mortality; D012720:Severity of Illness Index
D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D008565:Membrane Proteins; C021623:bactericidal permeability increasing protein