title: Intracellular dynamics of calcyon, a neuron-specific vesicular protein.
abstract: Calcyon is a brain-specific protein, implicated in clathrin-mediated endocytosis. In this descriptive study we show that calcyon is exclusively expressed in neurons, and localized in moving vesicles. The movement of calcyon-containing vesicles was dependent on temperature and on intact microtubules, in addition these vesicles were colocalized with a marker for endocytosed plasma membrane proteins, suggesting that calcyon vesicles follow the endocytic recycling pathway. We also show using evanescent wave microscopy that there is a pool of ready releasable calcyon vesiclesaccumulated beneath the plasma membrane. We conclude that the mobility and storage properties of calcyon-containing vesicles imply that they play a role in brain plasticity.
authors: Department of Woman and Child Health, Karolinska Institutet, Stockholm, Sweden.
authors: Markus
authors: M
authors: Kruusmägi
authors: Sergey
authors: S
authors: Zelenin
authors: Hjalmar
authors: H
authors: Brismar
authors: Lena
authors: L
authors: Scott
mesh_terms: D000818:Animals; D015415:Biomarkers; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002478:Cells, Cultured; D002966:Clathrin / Q000378:metabolism; D022163:Clathrin-Coated Vesicles / Q000378:metabolism / Q000648:ultrastructure; D003600:Cytosol / Q000378:metabolism / Q000648:ultrastructure; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D005456:Fluorescent Dyes; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D017072:Neostriatum / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology*; D014162:Transfection
chemical_list: D015415:Biomarkers; D002966:Clathrin; D005456:Fluorescent Dyes; D008565:Membrane Proteins; C405464:calcyon; D004247:DNA