title: Usefulness of cell counter-based parameters and formulas in detection of beta-thalassemia trait in areas of high prevalence.
abstract: The present study aimed to retrospectively evaluate the usefulness of cell counter-based parameters and formulas in beta-thalassemia trait (BTT) detection. The study included 170 BTT cases (hemoglobin [Hb]A(2) >4.0% [0.04]) and 30 non-BTT cases (HbA(2), 2.3%-3.5% [0.02-0.04]). Depending on the hemoglobin level and iron deficiency, the BTT group was further classified into classic BTT (n = 112) and BTT with iron deficiency anemia (n = 58). The RBC count, MCH, MCV, RDW, and Shine and Lal, Mentzler, Srivastava, England and Fraser, Ricerca, and Green indexes were applied. For the first time in the population of India, these 10 cell counter parameters and manual formulas were compared with high-performance liquid chromatography-derived HbA2 levels for deriving a cost-effective alternative method; and receiver operating characteristic curves were applied. We found that the Shine and Lal, Srivastava, and Mentzler indexes, MCV, and MCH have better discriminative function than the RBC count and red cell distribution width and their related formulas.
authors: Departments of Pathology, Smt NHL Municipal Medical College, Ahmedabad, India.
authors: Dinesh A
authors: DA
authors: Rathod
authors: Amarjeet
authors: A
authors: Kaur
authors: Vinod
authors: V
authors: Patel
authors: Kunjal
authors: K
authors: Patel
authors: Raviraj
authors: R
authors: Kabrawala
authors: Viral
authors: V
authors: Patel
authors: Mohak
authors: M
authors: Patel
authors: Pranay
authors: P
authors: Shah
mesh_terms: D018798:Anemia, Iron-Deficiency / Q000175:diagnosis* / Q000453:epidemiology / Q000235:genetics; D001772:Blood Cell Count / Q000295:instrumentation / Q000379:methods*; D015897:Comorbidity; D006443:Hemoglobin A2 / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007194:India; D011237:Predictive Value of Tests; D015995:Prevalence; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D017086:beta-Thalassemia / Q000175:diagnosis* / Q000453:epidemiology / Q000235:genetics
chemical_list: D006443:Hemoglobin A2