title: Drosophila DBT lacking protein kinase activity produces long-period and arrhythmic circadian behavioral and molecular rhythms.
abstract: A mutation (K38R) which specifically eliminates kinase activity was created in the Drosophila melanogaster ckI gene (doubletime [dbt]). In vitro, DBT protein carrying the K38R mutation (DBT(K/R)) interacted with Period protein (PER) but lacked kinase activity. In cell culture and in flies, DBT(K/R) antagonized the phosphorylation and degradation of PER, and it damped the oscillation of PER in vivo. Overexpression of short-period, long-period, or wild-type DBT in flies produced the same circadian periods produced by the corresponding alleles of the endogenous gene. These mutations therefore dictate an altered "set point" for period length that is not altered by overexpression. Overexpression of the DBT(K/R) produced effects proportional to the titration of endogenous DBT, with long circadian periods at lower expression levels and arrhythmicity at higher levels. This first analysis of adult flies with a virtual lack of DBT activity demonstrates that DBT's kinase activity is necessary for normal circadian rhythms and that a general reduction of DBT kinase activity does not produce short periods.
authors: School of Biological Sciences, University of Missouri-Kansas City, 5100 Rockhill Rd., Kansas City, MO 64110, USA.
authors: Michael J
authors: MJ
authors: Muskus
authors: Fabian
authors: F
authors: Preuss
authors: Jin-Yuan
authors: JY
authors: Fan
authors: Edward S
authors: ES
authors: Bjes
authors: Jeffrey L
authors: JL
authors: Price
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000235:genetics; D001522:Behavior, Animal / Q000502:physiology*; D048149:Casein Kinase 1 epsilon / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D002467:Cell Nucleus / Q000378:metabolism; D002940:Circadian Rhythm / Q000502:physiology*; D029721:Drosophila Proteins / Q000737:chemistry / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000201:enzymology*; D015870:Gene Expression; D005799:Genes, Dominant; D007814:Larva / Q000166:cytology / Q000378:metabolism; D008239:Lysine / Q000235:genetics; D008969:Molecular Sequence Data; D009043:Motor Activity / Q000502:physiology; D050505:Mutant Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D056950:Period Circadian Proteins; D010750:Phosphoproteins / Q000378:metabolism; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D013997:Time Factors
chemical_list: D029721:Drosophila Proteins; D050505:Mutant Proteins; D009687:Nuclear Proteins; C539040:PER protein, Drosophila; D056950:Period Circadian Proteins; D010750:Phosphoproteins; C113519:dco protein, Drosophila; D001120:Arginine; D011494:Protein Kinases; D048149:Casein Kinase 1 epsilon; D008239:Lysine