title: RNA interference-mediated gene silencing of vascular endothelial growth factor in colon cancer cells.
abstract: OBJECTIVE
  To inhibit the expression of vascular endothelial growth factor (VEGF) in colon cancer cell line by RNA interference (RNAi).
  
  
  METHODS
  Followed the service of E-RNAi, we designed and constructed two kinds of shRNA expression vectors aiming at the VEGF gene, then transfected them into colon cancer HT29 cells by lipofectamine(TM) 2000. The level of VEGF mRNA was investigated by RT-PCR and Northern blotting. The protein expression of VEGF was observed by immunofluoresence staining and Western blotting.
  
  
  RESULTS
  We got two kinds of VEGF specific shRNA expression vectors which could efficiently inhibit the expression of VEGF in HT29 cells. RT-PCR, Northern blotting, immunofluoresence staining and Western blotting showed that inhibition rate for VEGF expression was up to 42%, 89%, 73% and 82%, respectively.
  
  
  CONCLUSIONS
  The expression of VEGF can be inhibited by RNA interference in HT29 cells.
authors: Department in General Surgery, the First Affiliated Hospital, Chongqing University of Medical Science, Chongqing 400016, China.
authors: Tie-Jun
authors: TJ
authors: Li
authors: Jian-Ning
authors: JN
authors: Song
authors: Kai
authors: K
authors: Kang
authors: Shu-Sheng
authors: SS
authors: Tong
authors: Zan-Lan
authors: ZL
authors: Hu
authors: Tong-Chuan
authors: TC
authors: He
authors: Bing-Qiang
authors: BQ
authors: Zhang
authors: Cai-Quan
authors: CQ
authors: Zhang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D020868:Gene Silencing / Q000187:drug effects*; D005822:Genetic Vectors / Q000235:genetics; D019073:HT29 Cells; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D042461:Vascular Endothelial Growth Factor A