title: [Expression of 5 muscarinic receptor subtypes in the scleral tissue of immature guinea pigs].
abstract: OBJECTIVE
  To investigate the expression of 5 muscarinic receptor subtypes in the scleral tissue of immature guinea pigs.
  
  
  METHODS
  The scleral tissue was collected from six 2-week-old pigmented guinea pigs to determine mRNA expressions of the muscarinic receptors with RT-PCR. Immunofluorescence was used to observe the protein expressions of the 5 muscarinic receptor subtypes.
  
  
  RESULTS
  The mRNAs of the 5 muscarinic receptor subtypes were all detected in the scleral tissue, and the mRNA expression was the highest for M1 subtype. Green fluorescence of M1 to M5 subtypes surrounding the fibroblast nuclei were found in the scleral matrix using laser confocal microscopy.
  
  
  CONCLUSIONS
  Five muscarinic receptor subtypes are present in the scleral tissues of immature guinea pigs, indicating the involvement of the muscarinic receptors in eye growth regulation.
authors: State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
authors: Qiong
authors: Q
authors: Liu
authors: Jie
authors: J
authors: Wu
authors: Xin-mei
authors: XM
authors: Wang
authors: Jun-wen
authors: JW
authors: Zeng
mesh_terms: D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008297:Male; D009216:Myopia / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000235:genetics* / Q000378:metabolism*; D012590:Sclera / Q000166:cytology* / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D011976:Receptors, Muscarinic