title: Interaction between the keratin cytoskeleton and eEF1Bgamma affects protein synthesis in epithelial cells.
abstract: Eukaryotic elongation factor-1 (eEF1) is essential for peptide-chain elongation during translation. We report that its gamma subunit (eEF1Bgamma) specifically binds, and bundles, keratin intermediate filaments. Disrupting this interaction depresses translation by approximately 20% and selectively increases 80S ribosomes in epithelial cells, an outcome recapitulated by RNA interference-mediated silencing of eEF1Bgamma. These findings extend the emerging relationship between keratin proteins and the translational machinery.
authors: Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, Maryland 21205, USA.
authors: Seyun
authors: S
authors: Kim
authors: Juliane
authors: J
authors: Kellner
authors: Chang-Hun
authors: CH
authors: Lee
authors: Pierre A
authors: PA
authors: Coulombe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003599:Cytoskeleton / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007633:Keratins / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D020648:Peptide Elongation Factor 1; D021122:Protein Subunits; D007633:Keratins