title: Alterations of acetylcholinesterase activity after traumatic brain injury in rats.
abstract: OBJECTIVE
  The cholinergic system is highly vulnerable to traumatic brain injury (TBI). However, limited information is available to what extent the degrading enzyme acetylcholinesterase (AChE) is involved. The present study addresses this question.
  
  
  METHODS
  Thirty-six anaesthetized Sprague-Dawley rats were subjected to sham operation or to TBI using controlled cortical impact (CCI). The AChE activity was histochemically determined in frozen brain slices at 2, 24 and 72 hours after TBI.
  
  
  RESULTS
  High enzyme activity was observed in regions rich in cholinergic innervation such as the olfactory tubercle, basal forebrain, putamen and superior colliculi. Low activity was found in the cortex, cerebellum and particularly in the white matter. A decrease of AchE activity (20-35%) was found in the hippocampus and hypothalamus already at 2 hours after TBI. An increase of approximately 30% was found in the basal forebrain at 2 and 24 hours. No changes occurred at 72 hours.
  
  
  CONCLUSIONS
  The findings are consistent with impairment of the cholinergic neurotransmission after TBI and suggest the involvement of the AChE in short-term regulatory mechanisms.
authors: Institute of Interdisciplinary Isotope Research, Permoserstasse 15, 04318 Leipzig, Germany. donat@iif-leipzig.de
authors: Cornelius Kurt
authors: CK
authors: Donat
authors: Martin Ulrich
authors: MU
authors: Schuhmann
authors: Cornelia
authors: C
authors: Voigt
authors: Karen
authors: K
authors: Nieber
authors: Reinhard
authors: R
authors: Schliebs
authors: Peter
authors: P
authors: Brust
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D001930:Brain Injuries / Q000201:enzymology*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013570:Synaptic Membranes / Q000201:enzymology
chemical_list: D000110:Acetylcholinesterase