Collective diffusion coefficient of proteins with hydrodynamic, electrostatic, and adhesive interactions.
A theory is presented for lambdaC, the coefficient of the first-order correction in the density of the collective diffusion coefficient, for protein spheres interacting by electrostatic and adhesive forces. An extensive numerical analysis of the Stokesian hydrodynamics of two moving spheres is given so as to gauge the precise impact of lubrication forces. An effective stickiness is introduced and a simple formula for lambdaC in terms of this variable is put forward. A precise though more elaborate approximation for lambdaC is also developed. These and numerically exact expressions for lambdaC are compared with experimental data on lysozyme at pH 4.5 and a range of ionic strengths between 0.05M and 2M.
Prinsen
Peter
P
Complex Fluids Theory, Faculty of Applied Sciences, Delft University of Technology, 2628 BC Delft, The Netherlands.
Odijk
Theo
T
D000465:Algorithms; D001703:Biophysics / Q000379:methods; D002448:Cell Adhesion; D002627:Chemistry, Physical / Q000379:methods*; D004058:Diffusion; D006863:Hydrogen-Ion Concentration; D007477:Ions; D015233:Models, Statistical; D008962:Models, Theoretical; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry; D055672:Static Electricity; D014867:Water / Q000737:chemistry
D007477:Ions; D011506:Proteins; D014867:Water