Protein kinase C delta regulates anti-apoptotic alphaB-crystallin in the retina of type 2 diabetes.
We investigated the relationship between phosphorylation of alphaB-crystallin (alphaBC) and retinal apoptosis in type 2 diabetes. The retinas of male Otsuka Long-Evans Tokushima fatty (OLETF) rats at 24 and 35 weeks were used as an animal model for type 2 diabetes and sex- and age-matched Long-Evans Tokushima Otsuka (LETO) rats were used as controls. In the retinas of 35-week OLETF rats, the interaction between alphaBC and protein kinase C delta (PKC delta) among the PKC isozymes, alphaBC phosphorylation at Ser45 (S45p-alphaBC), TUNEL-positive apoptotic ganglion cells, several apoptotic signs, and co-localization of S45p-alphaBC and TUNEL significantly increased as compared with other groups while the alphaBC-Bax interaction greatly decreased. These changes were abolished by rottlerin treatment, a highly specific PKC delta inhibitor. These results suggest that PKC delta is involved in regulation of anti-apoptotic function of alphaBC in the retina of type 2 diabetes.
Kim
Y H
YH
Department of Anatomy and Neurobiology, School of Medicine, Institute of Health Science, Gyeongsang National University, Jinju, Chilam-dong 92, Jinju, Gyeongnam 660-751, South Korea.
Choi
M Y
MY
Kim
Y S
YS
Han
J M
JM
Lee
J H
JH
Park
C H
CH
Kang
S S
SS
Choi
W S
WS
Cho
G J
GJ
D000098:Acetophenones / Q000494:pharmacology; D000367:Age Factors; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001578:Benzopyrans / Q000494:pharmacology; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D003924:Diabetes Mellitus, Type 2 / Q000473:pathology*; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation; D047468:Immunoprecipitation / Q000379:methods; D020287:In Situ Nick-End Labeling; D008297:Male; D051745:Protein Kinase C-delta / Q000502:physiology*; D051381:Rats; D020334:Rats, Inbred OLETF; D020318:Rats, Long-Evans; D012160:Retina / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D038203:alpha-Crystallin B Chain / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D000098:Acetophenones; D001578:Benzopyrans; D001786:Blood Glucose; D004791:Enzyme Inhibitors; D038203:alpha-Crystallin B Chain; D051028:bcl-2-Associated X Protein; C085746:rottlerin; D051745:Protein Kinase C-delta