title: Classification of commercial Catuaba samples by NMR, HPLC and chemometrics.
abstract: For over a century, Catuaba has been used in Brazilian folk medicine as an aphrodisiac even though the identity of the plant material employed is often uncertain. The species recommended by the Brazilian Pharmacopeia is Anemopaegma arvense (Bignoniaceae), but many other plants, regionally known as Catuaba, are commercialised. Frequently, the quality control of such a complex system is based on chemical markers that do not supply a general idea of the system. With the advent of the metabolomics approach, a global analysis of samples becomes possible. It appears that (1)H-NMR is the most useful method for such application, since it can be used as a wide-spectrum chemical analysis technique. Unfortunately, the generated spectra is complex so a possible approach is to look at the metabolite profile as a whole using multivariate methods, for example, by application of principal component analysis (PCA). In the present paper, we describe for the first time a proton high-resolution magic angle spinning nuclear magnetic resonance ((1)H-HR-MAS NMR) method coupled with PCA for the metabolomic analysis of some commercial Catuaba samples, which provided a reduction in the time required for such analysis. A comparative study of HPLC, HR-MAS and liquid-NMR techniques is also reported.
authors: Departamento de Química, Universidade Federal de São Carlos, Rod. Washington Luis Km 235, São Carlos/SP, Brazil. Cristinadaolio@gmail.com
authors: Cristina
authors: C
authors: Daolio
authors: Flávio L
authors: FL
authors: Beltrame
authors: Antonio G
authors: AG
authors: Ferreira
authors: Quezia B
authors: QB
authors: Cass
authors: Diógenes Aparício Garcia
authors: DA
authors: Cortez
authors: Márcia M C
authors: MM
authors: Ferreira
mesh_terms: D001046:Aphrodisiacs / Q000737:chemistry; D029402:Bignoniaceae / Q000737:chemistry* / Q000145:classification; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D008519:Medicine, Traditional; D029582:Meliaceae / Q000737:chemistry* / Q000145:classification; D015999:Multivariate Analysis; D024301:Plant Bark / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry
chemical_list: D001046:Aphrodisiacs