title: Day 0 protocol: superstimulatory treatment initiated in the absence of a large follicle improves ovarian response and embryo yield in goats.
abstract: A new superstimulatory protocol (Day 0 Protocol) to initiate FSH treatment in the absence of a large follicle was compared to a traditional protocol in goats. The Day 0 Protocol (n=44) consisted of pre-treatment with progesterone and eCG to synchronize ovulation and the emergence of Wave 1, with FSH starting 84 h after the end of progesterone exposure (i.e., soon after ovulation). The traditional protocol (n=46) consisted of 11 d of progesterone exposure, with FSH treatment beginning 2 d before the end of progesterone exposure. Treatment with FSH was initiated in the absence of a large follicle in 37/44 and in 6/46 goats in the Day 0 Protocol and traditional protocol, respectively (P<0.01). There was more CL in the Day 0 Protocol than in the traditional protocol (breeding season: 9.6+/-0.6 and 6.3+/-0.8, P<0.05; non-breeding season: 14.3+/-1.5 and 10.7+/-1.5; P<0.05). More Grades 1 and 2 embryos were recovered in the Day 0 Protocol than in the traditional protocol (breeding season: 4.8+/-0.7 and 1.8+/-0.5, P<0.05; non-breeding season: 5.6+/-1.1 and 3.5+/-0.7, P=0.07). Similarly, the proportion of embryos that were Grades 1 and 2 was higher for the Day 0 Protocol than for the traditional protocol (breeding season: 81/114, 71%, versus 16/43, 37%, P<0.05; non-breeding season: 118/203, 58% versus 95/205, 46%, P<0.05). In summary, the Day 0 Protocol, was effective in initiating superstimulatory treatment in the absence of a large follicle, and compared to the traditional protocol, induced a higher ovulation rate and better embryo yield in goats.
authors: Instituto de Reproducción Animal de Uruguay, Montevideo, Uruguay. alejomen@adinet.com.uy
authors: A
authors: A
authors: Menchaca
authors: M
authors: M
authors: Vilariño
authors: M
authors: M
authors: Crispo
authors: A
authors: A
authors: Pinczak
authors: E
authors: E
authors: Rubianes
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D015237:Dinoprost / Q000008:administration & dosage / Q000031:analogs & derivatives; D004622:Embryo, Mammalian / Q000502:physiology; D004973:Estrus Synchronization / Q000379:methods*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage; D006041:Goats / Q000196:embryology / Q000502:physiology*; D006080:Ovarian Follicle / Q000000981:diagnostic imaging / Q000187:drug effects / Q000502:physiology*; D011374:Progesterone / Q000008:administration & dosage; D011461:Prostaglandins F, Synthetic / Q000008:administration & dosage; D027724:Reproductive Techniques, Assisted / Q000662:veterinary*; D013480:Superovulation; D013997:Time Factors; D014463:Ultrasonography
chemical_list: D006063:Chorionic Gonadotropin; D011461:Prostaglandins F, Synthetic; D011374:Progesterone; D005640:Follicle Stimulating Hormone; D015237:Dinoprost; C050851:delprostenate