title: Proteolysis and membrane capture of F-spondin generates combinatorial guidance cues from a single molecule.
abstract: The formation of neuronal networks is governed by a limited number of guidance molecules, yet it is immensely complex. The complexity of guidance cues is augmented by posttranslational modification of guidance molecules and their receptors. We report here that cleavage of the floor plate guidance molecule F-spondin generates two functionally opposing fragments: a short-range repellent protein deposited in the membrane of floor plate cells and an adhesive protein that accumulates at the basement membrane. Their coordinated activity, acting respectively as a short-range repellant and a permissive short-range attractant, constricts commissural axons to the basement membrane beneath the floor plate cells. We further demonstrate that the repulsive activity of the inhibitory fragment of F-spondin requires its presentation by the lipoprotein receptor-related protein (LRP) receptors apolipoprotein E receptor 2, LRP2/megalin, and LRP4, which are expressed in the floor plate. Thus, proteolysis and membrane interaction coordinate combinatorial guidance signaling originating from a single guidance cue.
authors: Department of Anatomy and Cell Biology, Hebrew University, Hadassah Medical School, Jerusalem 91120, Israel.
authors: Sophie
authors: S
authors: Zisman
authors: Karen
authors: K
authors: Marom
authors: Oshri
authors: O
authors: Avraham
authors: Lilah
authors: L
authors: Rinsky-Halivni
authors: Uri
authors: U
authors: Gai
authors: Gilit
authors: G
authors: Kligun
authors: Vered
authors: V
authors: Tzarfaty-Majar
authors: Tatsuo
authors: T
authors: Suzuki
authors: Avihu
authors: A
authors: Klar
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000166:cytology / Q000378:metabolism; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism*; D016764:Cell Polarity; D002642:Chick Embryo; D002645:Chickens; D002522:Chlorocebus aethiops; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D026502:LDL-Receptor Related Proteins; D051379:Mice; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D051381:Rats; D018110:Receptors, Lipoprotein / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology
chemical_list: D016326:Extracellular Matrix Proteins; D026502:LDL-Receptor Related Proteins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D018110:Receptors, Lipoprotein; C098773:low density lipoprotein receptor-related protein 8