title: Corneal wound healing from the perspective of keratoplasty specimens with special reference to the function of the Bowman layer and Descemet membrane.
abstract: OBJECTIVE
  To review corneal wound healing with special reference to the function of the Bowman layer and Descemet membrane.
  
  
  METHODS
  Corneal specimens were obtained from keratoplasties, including regrafted cases. Recipient corneal buttons were evaluated histopathologically with attention to 5 layers of corneal structure: 3 cellular layers consisting of epithelial cells, keratocytes, and endothelial cells and 2 acellular layers consisting of the Bowman layer and Descemet membrane.
  
  
  RESULTS
  Subepithelial fibrosis was found in advanced bullous keratopathy. The possible source of subepithelial fibrosis was either conjunctival stroma or corneal stroma through disruption of the Bowman layer. Subepithelial fibrosis was observed in the area of the Bowman layer disruption at the host-graft junction in regrafted cases. The Bowman layer was disrupted in eyes with not only keratoconus but also corneal dystrophy such as macular dystrophy and gelatinous drop-like dystrophy. Newly formed, thin Descemet membrane was found in keratoconic eyes of patients with acute hydrops. Retrocorneal membranes were observed in eyes with advanced bullous keratopathy and graft failure. Abnormal wound healing of Descemet membrane such as override and separation was found in the host-graft interface of regrafted eyes, causing stromal overgrowth.
  
  
  CONCLUSIONS
  The Bowman layer and Descemet membrane seem to serve as barriers to separate 3 cellular layers of epithelium, stroma, and endothelium. Disruption of the Bowman layer forms a new epithelial-stromal interaction and may cause cellular proliferative response. Separation of Descemet membrane can provide the trigger for emanating stromal tissue from the wound edge.
authors: Department of Ophthalmology, School of Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan. obatah@jichi.ac.jp
authors: Hiroto
authors: H
authors: Obata
authors: Tadahiko
authors: T
authors: Tsuru
mesh_terms: D050541:Bowman Membrane / Q000473:pathology*; D049109:Cell Proliferation; D003316:Corneal Diseases / Q000473:pathology / Q000601:surgery*; D016039:Corneal Transplantation / Q000009:adverse effects / Q000473:pathology*; D003886:Descemet Membrane / Q000473:pathology*; D005500:Follow-Up Studies; D005642:Fuchs' Endothelial Dystrophy / Q000209:etiology / Q000473:pathology; D006801:Humans; D011183:Postoperative Complications; D011379:Prognosis; D012189:Retrospective Studies; D014945:Wound Healing / Q000502:physiology*