Coumarin derivatives protection against ROS production in cellular models of Abeta toxicities.
The role of oxidative stress and free radicals in the development of Alzheimer's disease (AD) has been the focus of many recent studies. The role of hydrogen peroxide (H(2)O(2)) in AD is thought to be associated with Abeta (amyloid - beta) damage in cells. A number of coumarin derivatives were previously found to be potent anti-inflammatory and antioxidant agents. Herein, these coumarin derivatives were tested as H(2)O(2) scavengers with the DCF assay using two types of neuronal cells: (a) wild type (N2a) neuroblastoma cells and (b) APP/PS1 transgenic cell line expressing Abeta. Their scavenging activity was varied between the types of cell cultures and it was found to be concentration and time dependent in the mutant cells. Their protective role against cell death further supports this notion. These results suggest that these compounds could be used as a template in the design of new molecules with a possible role in AD.
Kontogiorgis
Christos A
CA
Aristotle University of Thessaloniki, School of Pharmacy, Department of Pharmaceutical Chemistry, Thessaloniki, Greece. kontog@pharm.auth.gr
Xu
Yanan
Y
Hadjipavlou-Litina
Dimitra
D
Luo
Yuan
Y
D000544:Alzheimer Disease / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000737:chemistry*; D000818:Animals; D045744:Cell Line, Tumor; D003374:Coumarins / Q000737:chemistry / Q000494:pharmacology*; D005609:Free Radicals; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D008822:Mice, Transgenic; D008956:Models, Chemical; D009154:Mutation; D018384:Oxidative Stress; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology
D016229:Amyloid beta-Peptides; D003374:Coumarins; D005609:Free Radicals; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles; C030123:coumarin; D006861:Hydrogen Peroxide; C022616:thiazolyl blue