Role of host protein Ebp1 in influenza virus growth: intracellular localization of Ebp1 in virus-infected and uninfected cells.
The cellular protein Ebp1 was identified to interact with PB1 protein of influenza virus RNA polymerase, and inhibit both RNA synthesis in vitro and influenza virus replication in vivo [Honda, A., Okamoto, T., Ishihama, A., 2007. Host factor Ebp1: selective inhibitor of influenza virus transcriptase. Genes Cells 12, 133-142]. The intracellular localization of Ebp1 that is involved in cell proliferation control was analyzed by direct immunostaining of cells before and after influenza virus infection. Ebp1 was found to localize in the nuclear membrane of uninfected cells, and to form nuclear aggregates with viral P proteins in virus-infected cells.
Honda
Ayae
A
Department of Frontier Bioscience and Micro-Nano-Technology Research Center, Hosei University, Koganei, Tokyo, Japan. ayhonda@hosei.ac.jp
D000818:Animals; D000914:Antibodies, Viral; D000918:Antibody Specificity; D004285:Dogs; D042541:Intracellular Space / Q000378:metabolism*; D009975:Orthomyxoviridae / Q000254:growth & development*; D009976:Orthomyxoviridae Infections; D011485:Protein Binding; D021122:Protein Subunits / Q000378:metabolism; D021381:Protein Transport; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism
D000914:Antibodies, Viral; D021122:Protein Subunits; D016601:RNA-Binding Proteins; D014764:Viral Proteins; C077346:influenza virus polymerase basic protein 1; D012324:RNA-Dependent RNA Polymerase