title: Bioreversibly crosslinked polyplexes of PEI and high molecular weight PEG show extended circulation times in vivo.
abstract: Copolymers consisting of branched PEI 25 kDa grafted with high molecular weight PEG at a low degree of substitution were successfully synthesized using a simple two-step procedure. The resulting AB-type and ABA-type copolymers were tested for cytotoxicity and DNA condensation and complexation properties. Their polyplexes with plasmid DNA were characterized in terms of DNA size and surface charge, transfection efficiency and blood compatibility. Pharmacokinetic profiles of the complexes containing (32)P-labeled plasmid were assessed before and after surface crosslinking. A set of four copolymers containing one or two PEG 20 kDa or PEG 30 kDa chains was obtained. The cytotoxicity of PEI was strongly reduced after copolymerization. The copolymer polyplexes showed hydrodynamic diameters of less than 200 nm, comparable to PEI 25. Similarly, no reduction in DNA condensation and complexation properties was found. In fact, PEI-PEG(30 k) copolymers exhibited better condensation and complexation properties than PEI 25. The transfection efficiency of copolymer polyplexes was increased 10-fold compared to PEI 25 control and the hemolytic activity was markedly reduced. After intravenous injection into mice, plasmids complexed to PEI-PEG(30 k) copolymers resulted in significantly increased circulation times. After stabilizing the polyplexes with a redox sensitive, biodegradable crosslinker, blood levels of plasmid could be further increased up to 125% compared to PEI. These results demonstrate that polyplexes prepared using a combined strategy of surface crosslinking and PEGylation seem to provide promising properties as stable, long circulating vectors.
authors: Department of Pharmaceutics and Biopharmacy, Philipps Universität, 35037 Marburg, Germany.
authors: Michael
authors: M
authors: Neu
authors: Oliver
authors: O
authors: Germershaus
authors: Martin
authors: M
authors: Behe
authors: Thomas
authors: T
authors: Kissel
mesh_terms: D001672:Biocompatible Materials / Q000008:administration & dosage / Q000009:adverse effects / Q000138:chemical synthesis / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000008:administration & dosage / Q000009:adverse effects / Q000138:chemical synthesis / Q000737:chemistry; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D008156:Luciferases / Q000235:genetics; D018613:Microscopy, Confocal; D008970:Molecular Weight; D010316:Particle Size; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000009:adverse effects / Q000138:chemical synthesis / Q000737:chemistry; D011094:Polyethyleneimine / Q000008:administration & dosage / Q000009:adverse effects / Q000138:chemical synthesis / Q000737:chemistry; D013997:Time Factors; D014162:Transfection
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D049452:Green Fluorescent Proteins; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D004247:DNA; C427203:calf thymus DNA; D008156:Luciferases