[Study on the application and evaluation of methods for gene and antigen detection in plague surveillance program].
OBJECTIVE
To apply and evaluate new methods regarding specific gene and antigen detection in plague surveillance program.


METHODS
1798 samples from natural foci of plague were tested, using internal quality control multiple-polymerase chain reaction, F1 antigen marked by immuno chromatographic assay and enzyme linked immunosorbent assay. Culture of Yersinia pestis and reverse indirect hemagglutination assay were used as reference diagnostic methods.


RESULTS
The overall positive rate of culture on Yersinia pestis together with gene and antigen detection was 7.34%, showing an 16.81% increase when comparing to 6.28% using Yersinia pestis culture method alone. The rate of coincidence was 97.13%.


CONCLUSIONS
The new standard being used for specific gene and antigen detection could increase the positive rate of diagnosis on plague.
Hai
Rong
R
State Kay Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Yu
Dong-zheng
DZ
Shi
Xian-ming
XM
Zhang
Zhong-bing
ZB
Tang
Yong-jiao
YJ
Wang
Peng
P
Xia
Lian-xu
LX
Wei
Shao-zhen
SZ
Xu
Bing
B
Qin
Ying-xu
YX
Zhang
Zhi-kai
ZK
Shi
Guo-xiang
GX
Xu Dong-lei
Cai
Hong
H
Zhang
En-min
EM
Wei
Jian-chun
JC
Geng
Ying-zhi
YZ
Huang
De-hui
DH
Zhao
Bin
B
Wang
Li-mao
LM
Ma
Feng-qin
FQ
Huang
Fu
F
Wang
Yue
Y
Zhang
Tao
T
Zhang
Jian-hua
JH
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D010930:Plague / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D015010:Yersinia pestis / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity
D001426:Bacterial Proteins; C086725:caf1 protein, Yersinia pestis