Bladder wall grafting in rats using salt-modified and collagen-coated polycaprolactone scaffolds: preliminary report.
OBJECTIVE
A rat model was used for the evaluation of collagen-coated and salt-modified polycaprolactone (PCL) scaffolds for bladder grafting after hemicystectomy.


METHODS
SD rats underwent partial cystectomy and cystoplasty with collagen-coated and salt-modified polycaprolactone scaffolds. The grafts of the regenerated bladder wall were harvested at different intervals and tissue regeneration was evaluated microscopically. Anatomic and functional characters were evaluated by cystography and urodynamics.


RESULTS
At harvesting, after 1 and 2 months, we found good preservation of the bladder shape and volume in all 16 rats receiving PCL cystorrhaphy. No stone formation was observed. Good epithelialization and ingrowth of smooth muscle cells were seen after 2 months grafting. Collagen-coated PCL scaffolds showed considerable encrustation, which appeared to be absorbed and disappear with time. The cystographic and urodynamic examinations revealed intact contour and a well-accommodated bladder with reservoir volume and contractility.


CONCLUSIONS
In the rat model, we have successfully demonstrated the applicability of collagen coated and salt-modified PCL in reconstruction of the partial cystectomized bladder.
Yu
Dah-Shyong
DS
Uro-Oncology Laboratory, Division of Urology, Department of Surgery, Tri-Service General Hospital, Taipei, Taiwan, Republic of China. yuds@ms21.hinet.net
Lee
Chi-Feng
CF
Chen
Hong-I
HI
Chang
Sun-Yran
SY
D020341:Absorbable Implants; D000818:Animals; D020099:Coated Materials, Biocompatible; D003094:Collagen; D015653:Cystectomy; D008297:Male; D011091:Polyesters; D051381:Rats; D017207:Rats, Sprague-Dawley; D019651:Plastic Surgery Procedures; D012965:Sodium Chloride; D023822:Tissue Engineering; D001743:Urinary Bladder / Q000637:transplantation*
D020099:Coated Materials, Biocompatible; D011091:Polyesters; C016240:polycaprolactone; D012965:Sodium Chloride; D003094:Collagen