title: Surfactant protein A regulates IgG-mediated phagocytosis in inflammatory neutrophils.
abstract: Surfactant proteins (SP)-A and SP-D have been shown to affect the functions of a variety of innate immune cells and to interact with various immune proteins such as complement and immunoglobulins. The goal of the current study is to test the hypothesis that SP-A regulates IgG-mediated phagocytosis by neutrophils, which are major effector cells of the innate immune response that remove invading pathogens by phagocytosis and by extracellular killing mediated by reactive oxygen and nitrogen. We have previously shown that SP-A stimulates chemotaxis by inflammatory, but not peripheral, neutrophils. To evaluate the ability of SP-A to modulate IgG-mediated phagocytosis, polystyrene beads were coated with BSA and treated with anti-BSA IgG. SP-A significantly and specifically enhanced IgG-mediated phagocytosis by inflammatory neutrophils, but it had no effect on beads not treated with IgG. SP-A bound to IgG-coated beads and enhanced their uptake via direct interactions with the beads as well as direct interactions with the neutrophils. SP-A did not affect reactive oxygen production or binding of IgG to neutrophils and had modest effects on polymerization of actin. These data suggest that SP-A plays an important role in mediating the phagocytic response of neutrophils to IgG-opsonized particles.
authors: Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
authors: Jessica A
authors: JA
authors: Wofford
authors: Jo Rae
authors: JR
authors: Wright
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007249:Inflammation / Q000473:pathology*; D008297:Male; D008863:Microspheres; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects*; D011485:Protein Binding / Q000187:drug effects; D037662:Pulmonary Surfactant-Associated Protein A / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D012995:Solubility / Q000187:drug effects
chemical_list: D000199:Actins; D007074:Immunoglobulin G; D037662:Pulmonary Surfactant-Associated Protein A; D017382:Reactive Oxygen Species; D009240:N-Formylmethionine Leucyl-Phenylalanine