Population patch clamp electrophysiology: a breakthrough technology for ion channel screening.
Population patch clamp (PPC) is a novel high throughput planar array electrophysiology technique that allows ionic currents to be recorded from populations of cells under voltage clamp. For the drug discovery pharmacologist, PPC promises greater speed and precision than existing methods for screening compounds at voltage-gated ion channel targets. Moreover, certain constitutively active or slow-ligand gated channels that have hitherto proved challenging to screen with planar array electrophysiology (e.g. SK/IK channels) are now more accessible. In this article we review early findings using PPC and provide a perspective on its likely impact on ion channel drug discovery. To support this, we include some new data on ion channel assay duplexing and on modulator assays, approaches that have thus far not been described.
Dale
Tim J
TJ
Biological Reagents & Assay Development, GlaxoSmithKline Research and Development, New Frontiers Science Park, Third Avenue, Harlow, Essex, UK. djt26940@gsk.com.
Townsend
Claire
C
Hollands
Emma C
EC
Trezise
Derek J
DJ
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004594:Electrophysiology; D006801:Humans; D015640:Ion Channel Gating; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D018408:Patch-Clamp Techniques / Q000295:instrumentation / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D007473:Ion Channels; D011994:Recombinant Proteins