IL-8 release of HL-60 cells treated with electric currents of different wave forms.
Human promyelocytic leukaemia HL-60 cells which have been differentiated by DMSO to granulocytes were used to investigate the effect of different waveforms on the release of interleukine-8 (IL-8). The cells were prestimulated with 100 pM fMLP and subsequently treated for 15 min with different electrical fields and currents. Three hours later the release of IL-8 into the medium was determined by ELISA. Varying the frequency of the sinusoidal electrical current between 0 and 20 Hz resulted in 2 maxima of IL-8 release at 5 and 13 Hz. Prestimulated cells were treated with sine-, triangular-, and rectangular-waveforms at 5 Hz in the current intensity range of 0-3 mA/cm(2). For the three waveforms tested, the IL-8 release was enhanced 1.5 fold. Treatment of the cells with capacitively coupled electric fields of 5 Hz using field strengths between 0 and 10 V(eff)/cm had no effect on the release of IL-8. In comparison to the positive results after sine wave exposure alone, an exposure with sine wave current to which noise had been superimposed had no effect on the HL-60 cells. From these investigations it can be concluded that for electrical current treatment of prestimulated HL-60 cells the release of IL-8 does not depend on the waveform if the waveform information is not destroyed by superimposed noise.
Sontag
Werner
W
Forschungszentrum Karlsruhe, Institut für Biologische Grenzflächen, Karlsruhe, Germany. Sontag@ibg.fzk.de
Weibezahn
Karl F
KF
D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation / Q000379:methods*; D004574:Electromagnetic Fields; D005786:Gene Expression Regulation / Q000528:radiation effects*; D018922:HL-60 Cells; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*
D016209:Interleukin-8