Accumulation of photofrin in lesions of airway stenosis rabbit models.
Airway stenosis in childhood is resistant to conventional treatments. Endoscope-assisted photodynamic therapy (PDT) is a potent candidate for the therapeutic modality owing to the easy approach to the tracheal lesion and low degree of invasiveness. The aim of the present study was to examine whether a photosensitizer preferentially accumulates in the lesion of airway stenosis in order to explore the possible applicability of PDT. The tracheal mucosa of rabbits was scraped off, and the rabbits were intravenously administered with Photofrin. The tissue concentration of Photofrin was quantitatively measured by fluorometric analysis. Granulation formation was seen in the mucosa-deprived lesion, causing airway stenosis. Photofrin concentration in the granulation tissue was four-fold higher than that in the intact trachea and 10-fold higher than that in the liver, spleen, skin and muscle. Photofrin preferentially accumulated in the lesion of airway stenosis. A preliminary experiment on PDT using transtracheal illumination showed an amelioration of airway stenosis, resulting in reduction in respiratory stridor.
Nakagishi
Yoshinori
Y
Department of Medical Engineering, National Defense Medical College, Tokorozawa, Saitama, Japan. yoshi710630@yahoo.co.jp
Morimoto
Yuji
Y
Fujita
Masanori
M
Ozeki
Yuichi
Y
Maehara
Tadaaki
T
Kikuchi
Makoto
M
D000818:Animals; D003251:Constriction, Pathologic / Q000378:metabolism*; D017323:Dihematoporphyrin Ether / Q000493:pharmacokinetics*; D004195:Disease Models, Animal; D008297:Male; D017319:Photosensitizing Agents / Q000493:pharmacokinetics*; D011817:Rabbits; D014018:Tissue Distribution; D014132:Trachea / Q000378:metabolism* / Q000473:pathology
D017319:Photosensitizing Agents; D017323:Dihematoporphyrin Ether