Electrically addressable diazonium-functionalized antibodies for multianalyte electrochemical sensor applications.
The direct electrically addressable deposition of diazonium-modified antibodies is examined for electrochemical immunosensing applications. The immobilized antibodies can be detected by the use of electroactive enzyme tags and nanoparticle-gold labeling. Control over antibody functionalization density and minimal spontaneous grafting of diazonium-antibody adducts is shown. The utility of the technique for a sandwich immunoassay as well as the ability to individually and selectively address closely spaced microelectrodes for multi-target protein detection in an array format is demonstrated.
Polsky
Ronen
R
Biosensors & Nanomaterials, Sandia National Laboratories, P.O. Box 5800, MS-0892, Albuquerque, NM 87185, USA.
Harper
Jason C
JC
Wheeler
David R
DR
Dirk
Shawn M
SM
Arango
Dulce C
DC
Brozik
Susan M
SM
D000906:Antibodies / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D016207:Cytokines / Q000032:analysis*; D003979:Diazonium Compounds / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006735:Horseradish Peroxidase / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D053758:Nanoparticles
D000906:Antibodies; D016207:Cytokines; D003979:Diazonium Compounds; D006735:Horseradish Peroxidase