title: Detection of microRNAs by Northern blot analyses using LNA probes.
abstract: MicroRNAs (miRNAs) are small, endogenous, non-coding RNA species, about 21 nucleotides in length, which modulate the expression of animal and plant target genes at the post-transcriptional level. It has been shown that miRNA based gene regulation plays a pivotal role in pathways involved in growth and development. Understanding miRNA mediated processes requires new technologies enabling efficient detection of small RNA species. Here we report the optimization of a miRNA Northern blot detection method based on LNA (locked nucleic acid)-modified oligonucleotide hybridization. This technique allows sensitive and highly specific detection of mature miRNAs.
authors: Agricultural Biotechnology Center, Szent-Györgyi Albert ut 4, Gödöllö H-2100, Hungary.
authors: Eva
authors: E
authors: Várallyay
authors: József
authors: J
authors: Burgyán
authors: Zoltán
authors: Z
authors: Havelda
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000235:genetics; D015152:Blotting, Northern / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification
chemical_list: D035683:MicroRNAs; D015345:Oligonucleotide Probes; D018749:RNA, Plant