Two distinct human POM121 genes: requirement for the formation of nuclear pore complexes.
Pom121 is one of the integral membrane components of the nuclear pore complex (NPC) in vertebrate cells. Unlike rodent cells carrying a single POM121 gene, human cells possess multiple POM121 gene loci on chromosome 7q11.23, as a consequence of complex segmental-duplications in this region during human evolution. In HeLa cells, two "full-length" Pom121 are transcribed and translated by two distinct genetic loci. RNAi experiments showed that efficient depletion of both Pom121 proteins significantly reduces assembled NPCs on nuclear envelope. Pom121-depletion also induced clustering of NPCs, indicating its role on maintenance of NPC structure/organization.
Funakoshi
Tomoko
T
Cellular Dynamics Laboratory, Discovery Research Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Maeshima
Kazuhiro
K
Yahata
Kazuhide
K
Sugano
Sumio
S
Imamoto
Fumio
F
Imamoto
Naoko
N
D024506:5' Flanking Region; D001483:Base Sequence; D002467:Cell Nucleus / Q000648:ultrastructure; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005091:Exons; D020440:Gene Duplication; D006367:HeLa Cells; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D022022:Nuclear Pore / Q000648:ultrastructure*; D017434:Protein Structure, Tertiary; D034622:RNA Interference
D008562:Membrane Glycoproteins; C094157:POM121 protein, human