Molecular characterisation and expression of Atlantic cod (Gadus morhua) myoglobin from two populations held at two different acclimation temperatures.
Much previous research has demonstrated the plasticity of myoglobin concentrations in both cardiac and skeletal myocytes in response to hypoxia and training. No study has yet looked at the effect of thermal acclimation on myoglobin in fish. Atlantic cod (Gadus morhua) from two different populations, i.e. the North Sea and the North East Arctic, were acclimated to 10 and 4 degrees C. Both the myoglobin mRNA and myoglobin protein in cod hearts increased significantly by up to 3.7 and 2.3 fold respectively as a result of acclimation to 4 degrees C. These increments were largest in the Arctic population, which in earlier studies have been shown to possess cold compensated metabolic demands at low temperatures. These metabolic demands associated with higher mitochondrial capacities may have driven the increase in cardiac myoglobin concentrations, in order to support diffusive oxygen supply. At the same time the increase in myoglobin levels may serve further functions during cold acclimation, for example, protection of the cell against reactive oxygen species, and scavenging nitric oxide, thereby contributing to the regulation of mitochondrial volume density.
Lurman
Glenn J
GJ
Alfred-Wegener-Institut fuer Polar-und Meeresforschung, Am Handelshafen 12, 27570, Bremerhaven, Germany. Glenn.Lurman@awi.de
Koschnick
Nils
N
Pörtner
Hans-O
HO
Lucassen
Magnus
M
D000064:Acclimatization; D000595:Amino Acid Sequence; D000818:Animals; D001110:Arctic Regions; D004734:Energy Metabolism; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D048389:Gadus morhua / Q000378:metabolism / Q000502:physiology*; D008929:Mitochondria, Heart / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D009211:Myoglobin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009659:North Sea; D010100:Oxygen / Q000378:metabolism; D011487:Protein Conformation; D012333:RNA, Messenger / Q000378:metabolism; D013696:Temperature; D015854:Up-Regulation
D029941:Fish Proteins; D009211:Myoglobin; D012333:RNA, Messenger; D010100:Oxygen