Improved (R)-phenylacetylcarbinol production with Candida utilis pyruvate decarboxylase at decreased organic to aqueous phase volume ratios.
The effect of decreasing the organic (octanol) to aqueous phase volume ratio was evaluated in a two-phase enzymatic process for (R)-phenylacetylcarbinol (PAC) production. Decreasing the ratio from 1:1 to 0.43:1 at 4 degrees C increased PAC in the organic phase from 112 g/l to 183 g/l with a 10% improvement in overall productivity. Interestingly, the rate of enzyme (pyruvate decarboxylase) activity loss was unaffected by the reduced phase ratio over the reaction period (48 h). At 20 degrees C and 0.43:1 phase ratio the organic phase PAC concentration increased to 212 g/l and the overall productivity increased by 30% although the PAC yield (based on pyruvate) declined by about 10% due to greater byproduct acetoin formation at the higher temperature. Product recovery in such a system is facilitated both by the higher PAC concentration and the reduced organic phase volume.
Gunawan
Cindy
C
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
Breuer
Michael
M
Hauer
Bernhard
B
Rogers
Peter L
PL
Rosche
Bettina
B
D000096:Acetone / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D002175:Candida / Q000201:enzymology*; D005591:Chemical Fractionation / Q000379:methods; D000442:Octanols / Q000737:chemistry; D011767:Pyruvate Decarboxylase / Q000737:chemistry*; D012995:Solubility; D013696:Temperature; D014867:Water / Q000737:chemistry*
D000442:Octanols; D014867:Water; D000096:Acetone; C035293:1-hydroxy-1-phenyl-2-propanone; D011767:Pyruvate Decarboxylase