title: Real-time atomic force microscopy reveals cytochrome c-induced alterations in neutral lipid bilayers.
abstract: The interaction of cytochrome c (cyt c) with supported lipid membranes was investigated on the nanoscale by real-time atomic force microscopy. Cyt c promoted the formation and the expansion of depressed areas in the fluid parts of the bilayer. When the depressions reached the gel domains, they induced the thickening of their edges. According to the step-height differences, cyt c was able to remove neutral lipids in the fluid phase and then to reside on the mica surface. Concerning gel phases, cyt c might insert between the two lipid leaflets, or it might intercalate between the mica and the bilayer.
authors: Laboratoire de Génie Enzymatique et Cellulaire, UMR-CNRS 6022, Université de Technologie de Compiègne, BP 20529, 60205 Compiègne Cedex, France.
authors: Sandrine
authors: S
authors: Morandat
authors: Karim
authors: K
authors: El Kirat
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000538:Aluminum Silicates; D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism; D005782:Gels; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D044085:Microfluidics; D018625:Microscopy, Atomic Force; D036103:Nanotechnology; D010713:Phosphatidylcholines / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000538:Aluminum Silicates; D005782:Gels; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D015060:1,2-Dipalmitoylphosphatidylcholine; D045304:Cytochromes c; C017251:1,2-oleoylphosphatidylcholine; C011934:mica