Topical dorsal skin immersion in seawater induces apoptosis and proliferation in hairless mice.
Recreational and occupational exposure to seawater (SW), have increased but the effect of SW on skin has not been elucidated. The purpose of present study was to assess the effects of SW immersion on the dorsal skin in hairless mice. Adult hairless mice were individually immersed in SW for 3 h, 6 h and 12 h; then, full-thickness dorsal skin of 2 cm diameter was excised for pathological examination (light microscope), apoptosis detection (terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end labeling [TUNEL]) and proliferation index evaluation (immunohistochemistry). Normal and normal saline (NS)-immersed skin were used as controls. Histological examination revealed that there were randomly distributed cell deaths, presenting cell shrinkage, condensation of nuclear chromatin and eosinophilic cytoplasm in the epidermis, and neutrophil infiltration in the dermis, after SW immersion. Moreover, TUNEL showed low levels of apoptosis in normal (9.07 +/- 0.70%) and NS-immersed skin (9.99 +/- 1.22%). There was an apparent increase in the 6-h and 12-h SW immersed groups (29.90 +/- 6.85%, P < 0.01; 45.46 +/- 6.12%, P < 0.01, respectively). Ki-67 antigen was located in the basal layer of the epidermis and hair follicles, the rates of Ki-67-positive cells were 7.90 +/- 1.45% and 7.76 +/- 1.52% in normal and NS-immersed skin, respectively, and in the 12-h SW immersed group, the rate of Ki-67-positive cells reached 23.85 +/- 4.21% (threefold, P < 0.01). In each group, the rate of apoptosis was higher than that of proliferation. We conclude that SW immersion can cause time-dependent apoptosis and proliferation in the epidermis, and the overall effect of SW immersion is injury to the epidermis.
Pan
Min Hong
MH
Clinical School of Medical College of Nanjing University and Department of Pathology, Jinling Hospital, Nanjing, Jiangsu, China.
Jiang
Shao Jun
SJ
Liu
Xiao Hong
XH
Rao
Qiu
Q
Zhou
Zhi Yi
ZY
Ma
Heng Hui
HH
Zhou
Xiao Jun
XJ
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D049109:Cell Proliferation; D006801:Humans; D007101:Immersion / Q000009:adverse effects; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D019394:Ki-67 Antigen / Q000032:analysis; D051379:Mice; D008812:Mice, Hairless; D012623:Seawater / Q000009:adverse effects*; D012867:Skin / Q000166:cytology*
D019394:Ki-67 Antigen