title: Src-dependent phosphorylation of ASAP1 regulates podosomes.
abstract: Invadopodia are Src-induced cellular structures that are thought to mediate tumor invasion. ASAP1, an Arf GTPase-activating protein (GAP) containing Src homology 3 (SH3) and Bin, amphiphysin, and RVS161/167 (BAR) domains, is a substrate of Src that controls invadopodia. We have examined the structural requirements for ASAP1-dependent formation of invadopodia and related structures in NIH 3T3 fibroblasts called podosomes. We found that both predominant splice variants of ASAP1 (ASAP1a and ASAP1b) associated with invadopodia and podosomes. Podosomes were highly dynamic, with rapid turnover of both ASAP1 and actin. Reduction of ASAP1 levels by small interfering RNA blocked formation of invadopodia and podosomes. Podosomes were formed in NIH 3T3 fibroblasts in which endogenous ASAP1 was replaced with either recombinant ASAP1a or ASAP1b. ASAP1 mutants that lacked the Src binding site or GAP activity functioned as well as wild-type ASAP1 in the formation of podosomes. Recombinant ASAP1 lacking the BAR domain, the SH3 domain, or the Src phosphorylation site did not support podosome formation. Based on these results, we conclude that ASAP1 is a critical target of tyrosine kinase signaling involved in the regulation of podosomes and invadopodia and speculate that ASAP1 may function as a coincidence detector of simultaneous protein association through the ASAP1 SH3 domain and phosphorylation by Src.
authors: Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA.
authors: Sanita
authors: S
authors: Bharti
authors: Hiroki
authors: H
authors: Inoue
authors: Kapil
authors: K
authors: Bharti
authors: Dianne S
authors: DS
authors: Hirsch
authors: Zhongzhen
authors: Z
authors: Nie
authors: Hye-Young
authors: HY
authors: Yoon
authors: Vira
authors: V
authors: Artym
authors: Kenneth M
authors: KM
authors: Yamada
authors: Susette C
authors: SC
authors: Mueller
authors: Valarie A
authors: VA
authors: Barr
authors: Paul A
authors: PA
authors: Randazzo
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000378:metabolism*; D020816:Amino Acid Motifs; D000818:Animals; D045744:Cell Line, Tumor; D021961:Cell Membrane Structures / Q000201:enzymology*; D051356:Cortactin / Q000378:metabolism; D020690:GTPase-Activating Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D050505:Mutant Proteins / Q000378:metabolism; D041681:NIH 3T3 Cells; D010748:Phosphopeptides / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding; D020033:Protein Isoforms / Q000378:metabolism; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C503238:Arfgap1 protein, mouse; C485546:Asap1 protein, mouse; D051356:Cortactin; D020690:GTPase-Activating Proteins; D050505:Mutant Proteins; D010748:Phosphopeptides; D020033:Protein Isoforms; D014443:Tyrosine; D016392:Proto-Oncogene Proteins pp60(c-src)