title: Role of calcium mobilization in the regulation of spontaneous transient outward currents in porcine coronary artery myocytes.
abstract: The purpose of the present study was to further study the characteristics and regulation of spontaneous transient outward currents (STOCs) in freshly isolated porcine coronary artery smooth muscle cells (ASMCs). STOCs were recorded using the perforated whole-cell patch-clamp configuration. STOCs were voltage-dependent and superimposed stochastically onto whole-cell Ca(2+)-activated-K(+) (BK(Ca)) currents. Charybdotoxin (ChTX, 200 nmol/L), a selective blocker of BK(Ca) channels, completely inhibited STOCs within 10 min. STOCs activity was greatly suppressed when extracellular Ca(2+) concentration decreased from 1.8 mmol/L to 200 nmol/L, further removal of Ca(2+) abolished STOCs activity. Ca(2+) ionophore A23187 (10 micromol/L) increased STOCs activity significantly. Verapamil (20 micromol/L) and CdCl(2) (200 micromol/L), two kinds of organic L-type voltage-dependent Ca(2+) channels (L-VDCCs) antagonists, had little effect on STOCs. In addition, the ryanodine receptors (RyRs) agonist caffeine (5 mmol/L) significantly activated STOCs. Application of ryanodine (50 micromol/L) to block RyRs abolished STOCs, subsequent washout of ryanodine or application of caffeine failed to reproduce STOCs activity. Inhibition of inositol 1,4,5-trisphosphate receptors (IP(3)Rs) by 2APB (40 micromol/L) greatly suppressed the activity of STOCs, application of caffeine (5 mmol/L) in the presence of 2APB caused a burst of outward currents followed by inhibition of STOCs. These results suggest that STOCs in porcine coronary ASMCs are mediated by BK(Ca) channels. Extracellular Ca(2+) is essential for STOCs activity, while Ca(2+) entry through L-VDCCs has little effect on STOCs. Intracellular Ca(2+) release induced by RyRs is responsible for the regulation of STOCs, whereas IP3Rs might also be involved.
authors: Institute of Myocardial Electrophysiology, Luzhou Medical College, Luzhou 646000, China.
authors: PengYun
authors: P
authors: Li
authors: XiaoRong
authors: X
authors: Zeng
authors: Yan
authors: Y
authors: Yang
authors: Fang
authors: F
authors: Cai
authors: ZhiFei
authors: Z
authors: Liu
authors: MiaoLing
authors: M
authors: Li
authors: Jie
authors: J
authors: Pei
authors: Wen
authors: W
authors: Zhou
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D018999:Charybdotoxin / Q000494:pharmacology; D003331:Coronary Vessels / Q000378:metabolism*; D004594:Electrophysiology; D015544:Inositol 1,4,5-Trisphosphate / Q000737:chemistry; D015233:Models, Statistical; D032342:Muscle Cells / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D009498:Neurotoxins / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D012433:Ryanodine / Q000494:pharmacology; D013552:Swine
chemical_list: D002121:Calcium Channel Blockers; D009498:Neurotoxins; D018999:Charybdotoxin; D012433:Ryanodine; D002110:Caffeine; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium