title: Sustained Arc/Arg3.1 synthesis controls long-term potentiation consolidation through regulation of local actin polymerization in the dentate gyrus in vivo.
abstract: New gene expression is necessary for long-term potentiation (LTP) consolidation, yet roles for specific activity-induced mRNAs have not been defined. Here we probed the dynamic function of activity-induced Arc (activity-regulated cytoskeletal-associated protein)/Arg3.1 (activity-regulated gene 3.1 protein homolog) mRNA using brief, local infusions of antisense (AS) oligodeoxynucleotides at multiple time points during dentate gyrus LTP in vivo. Surprisingly, early Arc synthesis is necessary for early expression of LTP, whereas sustained synthesis is required to generate stably modified synapses. AS application 2 h after LTP induction results in a rapid and permanent reversal of LTP. This reversal is associated with rapid knockdown of upregulated Arc, dephosphorylation of actin depolymerization factor/cofilin, and loss of nascent filamentous actin (F-actin) at synaptic sites. Infusion of the F-actin stabilizing drug jasplakinolide during LTP maintenance blocks the ability of AS to reverse LTP. These results couple activity-induced expression of Arc to expansion of the actin cytoskeleton underlying enduring LTP. Furthermore, Arc synthesis is required for both the induction and consolidation of LTP elicited by local BDNF infusion, thus identifying Arc as a key molecular effector of BDNF in synaptic plasticity.
authors: Department of Biomedicine and Bergen Mental Health Research Center, University of Bergen, N-5009 Bergen, Norway.
authors: Elhoucine
authors: E
authors: Messaoudi
authors: Tambudzai
authors: T
authors: Kanhema
authors: Jonathan
authors: J
authors: Soulé
authors: Adrian
authors: A
authors: Tiron
authors: Girstaute
authors: G
authors: Dagyte
authors: Bruno
authors: B
authors: da Silva
authors: Clive R
authors: CR
authors: Bramham
mesh_terms: D051339:Actin Depolymerizing Factors / Q000502:physiology; D000199:Actins / Q000378:metabolism*; D000818:Animals; D001704:Biopolymers / Q000502:physiology; D019208:Brain-Derived Neurotrophic Factor / Q000502:physiology; D003598:Cytoskeletal Proteins / Q000096:biosynthesis*; D018891:Dentate Gyrus / Q000502:physiology*; D017774:Long-Term Potentiation / Q000502:physiology*; D008297:Male; D023421:Models, Animal; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009473:Neuronal Plasticity / Q000502:physiology; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D009435:Synaptic Transmission / Q000502:physiology
chemical_list: D051339:Actin Depolymerizing Factors; D000199:Actins; D001704:Biopolymers; D019208:Brain-Derived Neurotrophic Factor; D003598:Cytoskeletal Proteins; D009419:Nerve Tissue Proteins; C092069:activity regulated cytoskeletal-associated protein