Near IR-emitting DNA-probes exploiting stepwise energy transfer processes.
The synthesis and characterisation of two new cyclen-based near IR-emitting lanthanide complexes is reported; the lanthanides are sensitised by rhodamine, which in turn is excited by energy transfer from a coumarin 2 moiety. The three lumophores function as an energy transfer cascade spanning the UV-visible-near IR region of the spectrum, resulting in large Stokes shifts. Double stranded DNA selectively switches one of the two energy transfer processes off, enabling luminescent DNA-sensing in the near IR region. The regioselective di-alkylation of the cyclen scaffold is explained with the help of DFT calculations.
Bodi
Andras
A
Molecular Dynamics Group, Paul Scherrer Institut, Villigen, 5232, Switzerland.
Borbas
K Eszter
KE
Bruce
James I
JI
D000042:Absorption; D000478:Alkylation; D004247:DNA / Q000378:metabolism*; D004735:Energy Transfer; D007259:Infrared Rays; D028581:Lanthanoid Series Elements / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D047028:Macrocyclic Compounds / Q000737:chemistry; D011789:Quantum Theory; D013237:Stereoisomerism; D013379:Substrate Specificity
D028581:Lanthanoid Series Elements; D047028:Macrocyclic Compounds; D004247:DNA