title: Molecular cloning, characterization, and developmental expression of foxp1 in zebrafish.
abstract: The forkhead transcription factor gene family encodes a large group of proteins that play critical roles in many developmental events. In this report, we describe the isolation and characterization of foxp1 in zebrafish. The full-length zebrafish foxp1 cDNA contains 2,440 bp encoding a protein of 659 amino acids which shares 72, 68, 68, 70, 65, and 62% overall identity with human, mouse, rat, chicken, zebra finch, and frog Foxp1, respectively. Results of whole-mount in situ hybridization showed that foxp1 exhibits very complex and dynamic expression pattern during early embryonic development. Prominent foxp1 expression is detected in many regions of the developing central nervous system, especially in midbrain-hindbrain boundary, hindbrain, and spinal cord. Strong expression is also observed in retina, ear, branchial arches, hatching gland, heart, pronephric duct, gut, proctodeum, pectoral fin, and swim bladder. These results provide evidence that foxp1 is likely to function as a very important transcription factor in the development of the central nervous system and many other organs in zebrafish.
authors: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, 200433, People's Republic of China.
authors: Lu
authors: L
authors: Cheng
authors: Mei
authors: M
authors: Chong
authors: Weiwei
authors: W
authors: Fan
authors: Xiaofang
authors: X
authors: Guo
authors: Wenjuan
authors: W
authors: Zhang
authors: Xueyan
authors: X
authors: Yang
authors: Fan
authors: F
authors: Liu
authors: Yonghao
authors: Y
authors: Gui
authors: Daru
authors: D
authors: Lu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D051858:Forkhead Transcription Factors / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D051858:Forkhead Transcription Factors; D029961:Zebrafish Proteins