title: [Effects of Rehmannia glutinosa oligosaccharides on proliferation of HepG2 and insulin resistance].
abstract: OBJECTIVE
  To investigate the effects of Rehmannia glutinosa oligosaccharides (ROS) on the proliferation of HepG2 and insulin resistance.
  
  
  METHODS
  The HepG2 cells were divided into control group, rosiglitazone (3.4 mg x L(-1)) treated group and ROS (0.1-30 mg x L(-1)) treated group. The proliferation of HepG2 was detected by MTT method. Insulin resistant HepG2 cells model was induced by high concentration of insulin, then the effects of ROS on glucose consumption in insulin resistant HepG2 cells were investigated.
  
  
  RESULTS
  In the middle glucose culture medium, the absorbance at 570 nm of HepG2 was increased by high concentration of ROS, and decreased by low concentration of ROS by using MTT method, a concentration-dependent manner. ROS increased glucose consumption in HepG2 cells, and showed a better effect at the dose of 10 mg x L(-1). ROS promoted the glucose consumption in insulin resistance of HepG2 cells, improved the sensitivity of insulin resistance of HepG2 cells to insulin.
  
  
  CONCLUSIONS
  High concentration of ROS can promote the proliferation of HepG2, and however low concentration of ROS inhibits the proliferation of HepG2. ROS can significantly improve insulin resistance of HepG2 cells induced by high insulin.
authors: Life Science Institute of Lanzhou University, Lanzhou 730000, China.
authors: Li-min
authors: LM
authors: Guo
authors: Ru-xue
authors: RX
authors: Zhang
authors: Zheng-ping
authors: ZP
authors: Jia
authors: Mao-xing
authors: MX
authors: Li
authors: Juan
authors: J
authors: Wang
authors: Qiang
authors: Q
authors: Yin
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D019008:Drug Resistance, Neoplasm; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007333:Insulin Resistance; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D009844:Oligosaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D032267:Rehmannia / Q000737:chemistry*
chemical_list: D007004:Hypoglycemic Agents; D007328:Insulin; D009844:Oligosaccharides; D005947:Glucose