Environmental monitoring of Mycobacterium bovis in badger feces and badger sett soil by real-time PCR, as confirmed by immunofluorescence, immunocapture, and cultivation.
Real-time PCR was used to detect and quantify Mycobacterium bovis cells in naturally infected soil and badger feces. Immunomagnetic capture, immunofluorescence, and selective culture confirmed species identification and cell viability. These techniques will prove useful for monitoring M. bovis in the environment and for elucidating transmission routes between wildlife and cattle.
Sweeney
F P
FP
Microbiology Group, Department of Biological Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, United Kingdom. f.p.sweeney@warwick.ac.uk
Courtenay
O
O
Hibberd
V
V
Hewinson
R G
RG
Reilly
L A
LA
Gaze
W H
WH
Wellington
E M H
EM
D000818:Animals; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics; D004784:Environmental Monitoring; D005243:Feces / Q000382:microbiology*; D005455:Fluorescent Antibody Technique; D050296:Microbial Viability; D045969:Mustelidae / Q000382:microbiology*; D009163:Mycobacterium bovis / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012988:Soil Microbiology
D004269:DNA, Bacterial