[Experiment on inducing human dental pulp stem cells into neural-like cells].
OBJECTIVE
To explore the multi-differentiated capability of human dental pulp stem cells (hDPSCs) obtained by cell-clone culture approach and to determine the appropriate induced medium.


METHODS
The cloned isolation and expansion of hDPSCs were preinduced for 24 h, and were subsequently replaced with neural-inductive medium containing certain concentration of dimethylsulfoxide (DMSO), butylated hydroxyanisode (BHA), forskolin, P-mercaptoethanol (p-ME) and hydrocortisone for 4 days. Then induced cells were analyzed by morphological observation, immnocytochemical staining for non-specific esterase (NSE) and glial fibrillary acidic protein (GFAP) expression, RT-PCR for GFAP mRNA. Meanwhile, the uninduced hDPSCs were used as negative control.


RESULTS
The morphology of induced cells changed at the initial 12 h, and displayed a typical neuron-like cells at 24 h. There was a gradual increase in the number of these neuronal differentiated cells with continuous induction. Furthermore, immnocytochemical staining showed that the induced cell expressed NSE and GFAP, two marked enzymes of neuron cell. The GFAP mRNA was also detected in induced cells by RT-PCR assay. In contrast, the uninduced cells maintained its original appearance and had no expression on them.


CONCLUSIONS
hDPSCs may possess potential of multiple-differentiation and may differentiate into neuron-like cells on certain inductive condition.
He
Hui-xia
HX
Research and Development Center for Tissue Engineering, Dept. of Oral Histology and Pathology, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Jin
Yan
Y
Shi
Jun-nan
JN
Luo
Yu-qing
YQ
Zhou
Yan-ni
YN
Peng
Zhi
Z
Xu
Yu-he
YH
D001854:Bone Marrow Cells; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D003782:Dental Pulp; D004847:Epithelial Cells; D006801:Humans; D059630:Mesenchymal Stem Cells; D009474:Neurons; D013234:Stem Cells