New properties of Drosophila fab-7 insulator.
In the Abd-B 3' cis-regulatory region, which is subdivided into a series of iab domains, boundary elements have previously been detected, including the Fab-7 element providing for the autonomous functioning of the iab-6 and iab-7 cis-regulatory domains. Here, it has been shown that a single copy of the 860-bp Fab-7 insulator effectively blocks the yellow and white enhancers. The eye and testis enhancers can stimulate the white promoter across the pair of Fab-7, which is indicative of a functional interaction between the insulators. Unexpectedly, Fab-7 has proved to lose the enhancer-blocking activity when placed near the white promoter. It seems likely that Fab-7 strengthens the relatively weak white promoter, which leads to the efficient enhancer-promoter interaction and insulator bypass.
Rodin
Sergey
S
Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.
Kyrchanova
Olga
O
Pomerantseva
Ekaterina
E
Parshikov
Alexander
A
Georgiev
Pavel
P
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000818:Animals; D030801:Animals, Genetically Modified; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D005123:Eye / Q000166:cytology / Q000378:metabolism; D005136:Eye Proteins / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D038101:Insulator Elements / Q000235:genetics*; D008297:Male; D011401:Promoter Regions, Genetic; D019076:Transgenes / Q000502:physiology
D018528:ATP-Binding Cassette Transporters; D029721:Drosophila Proteins; D005136:Eye Proteins; C059254:w protein, Drosophila; C050680:y protein, Drosophila