title: Enterocytozoon bieneusi in mature dairy cattle on farms in the eastern United States.
abstract: Fecal specimens were obtained from mature milking cows on farms in Vermont, New York, Pennsylvania, Maryland, Virginia, North Carolina, and Florida. Polymerase chain reaction (PCR)-positive specimens for Enterocytozoon bieneusi were found in 24 of 541 cows examined (4.4%) and on 12 of 14 farms. The prevalence of E. bieneusi varied considerably from farm to farm, with the lowest prevalence (2.3%) on FL-2 and the highest prevalence (12.5%) on VT-2. None of the cows exhibited signs of diarrhea. All PCR-positive specimens were sequenced to determine the genotype of E. bieneusi. Five genotypes were identified. Three were identified as cattle-specific genotypes previously reported as BEB1, BEB2, and BEB4, and two new genotypes, BEB 6 and BEB7, were found. None have been reported to infect humans.
authors: Environmental Microbial Safety Laboratory, Animal and Natural Resources Institute Agricultural Research Service, United States Department of Agriculture, Building 173, BARC-East, 10300 Baltimore Avenue, Beltsville, MD 20705, USA. rfayer@anri.barc.usda.gov
authors: R
authors: R
authors: Fayer
authors: M
authors: M
authors: Santín
authors: J M
authors: JM
authors: Trout
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D021862:Enterocytozoon / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D016881:Microsporidiosis / Q000453:epidemiology / Q000662:veterinary*; D015995:Prevalence; D014481:United States / Q000453:epidemiology