title: Quantitative analysis by HPLC-MS2 of the pyrrolizidine alkaloid adonifoline in Senecio scandens.
abstract: A quantitative method using HPLC-MS(2) has been developed for the determination of adonifoline, one of the retronecine-type hepatotoxic pyrrolizidine alkaloids in Senecio scandens Buch.-Ham. ex D. Don., a traditional Chinese herb. Using an orthogonal design test, a simple and rapid sample extraction method was developed. HPLC analysis was conducted using a C(18) column as stationary phase and a mixture of acetonitrile and aqueous formic acid as mobile phase. Good linearity for adonifoline was found in the concentration range 0.12-4.18 microg/mL, and the HPLC-MS/MS method was shown to be appropriate, in terms of sensitivity, precision and reproducibility. The quantities of adonifoline in extracts of 18 plant samples from different collection sources and from different parts (flowers, leaves, thick stems, slim stems and roots) of S. scandens were determined using the newly developed HPLC/MS(2) analysis.
authors: Key Laboratory of Standardisation of Chinese Medicines of Ministry of Education, Shanghai University of Traditional Chinese Medicine, Zhangjiang Hi-Tech Park, Shanghai 201203, People's Republic of China.
authors: Fang
authors: F
authors: Zhang
authors: Chang-Hong
authors: CH
authors: Wang
authors: Wan
authors: W
authors: Wang
authors: Lu-Xin
authors: LX
authors: Chen
authors: Hong-Yan
authors: HY
authors: Ma
authors: Chao-Feng
authors: CF
authors: Zhang
authors: Mian
authors: M
authors: Zhang
authors: S W Annie
authors: SW
authors: Bligh
authors: Zheng-Tao
authors: ZT
authors: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010946:Plants, Medicinal / Q000737:chemistry*; D011763:Pyrrolizidine Alkaloids / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012674:Senecio / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011763:Pyrrolizidine Alkaloids