Effects of lipopolysaccharide on newly established rat dental pulp-derived cell line with odontoblastic properties.
To clarify mechanisms of pulp wound healing and regeneration, it is important to establish continuous odontoblast-lineage cell lines. In this study, we established the proliferating pulp progenitor cell lines from dental papilla cells of rat incisor. These cell lines showed high levels of alkaline phosphatase (ALP) activity, expression of Runx2 and dentin sialophosphoprotein (DSPP), and extracellular formation of mineralized nodules. By using the cell line with high expression level of DSPP and the prominent mineral deposition, we examined whether bacterial lipopolysaccharide (LPS) had effects on its odontoblastic properties and found that ALP activity, expression of DSPP and Runx2, and the formation of mineralized nodules were suppressed in LPS dose-dependent manner. These results indicate that our established pulp progenitor cell line exhibits odontoblastic properties, which were suppressed by LPS, suggesting that gram-negative bacterial infection might downregulate the odontoblast function.
Nomiyama
Kimiko
K
Department of Health Promotion, Division of Infections and Molecular Biology, Kyushu Dental College, Fukuoka, Japan.
Kitamura
Chiaki
C
Tsujisawa
Toshiyuki
T
Nagayoshi
Masato
M
Morotomi
Takahiko
T
Terashita
Masmichi
M
Nishihara
Tatsuji
T
D016976:Aggregatibacter actinomycetemcomitans; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D019070:Cell Lineage; D002999:Clone Cells / Q000166:cytology; D050659:Core Binding Factor Alpha 1 Subunit; D003771:Dental Papilla / Q000166:cytology; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D016326:Extracellular Matrix Proteins; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D009804:Odontoblasts / Q000166:cytology / Q000187:drug effects*; D010750:Phosphoproteins; D011498:Protein Precursors; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins; D013047:Specific Pathogen-Free Organisms; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors; D000099311:Dentin Sialophosphoprotein
D000469:Alkaline Phosphatase; D002118:Calcium; D050659:Core Binding Factor Alpha 1 Subunit; D016326:Extracellular Matrix Proteins; D008070:Lipopolysaccharides; D010750:Phosphoproteins; D011498:Protein Precursors; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C493717:Runx2 protein, rat