title: A transgenic mouse model for high content, cell cycle phenotype screening in live primary cells.
abstract: High content cell-based genetic and small molecule library screens are powerful strategies in drug discovery and investigations of disease mechanisms. We report that primary cells derived from a transgenic mouse model expressing a fluorescence mitosis biosensor provide unambiguous phenotype readouts without the need for transfection or immunocytochemistry. Phenotype profiles of cell cycle disruption and of apoptosis are easily detectable at a single time point selected from time-lapse live fluorescence microscopy. Most importantly, this transgenic mouse model may be crossed with cancer mouse models to derive biosensor-expressing primary cancer cells for use in high content screening strategies targeting discovery of tumor-specific chemotherapeutic compounds.
authors: Department of Gynecology and Obstetrics, Stanford University School of Medicine, Stanford, California 94305, USA.
authors: Richard O
authors: RO
authors: Burney
authors: Alan I
authors: AI
authors: Lee
authors: Denise E
authors: DE
authors: Leong
authors: Joshua T
authors: JT
authors: Jones
authors: Angela T
authors: AT
authors: Hahn
authors: Tobias
authors: T
authors: Meyer
authors: Mylene W M
authors: MW
authors: Yao
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D023421:Models, Animal; D010641:Phenotype; D011247:Pregnancy; D013045:Species Specificity