Antibiotic susceptibility testing of Bifidobacterium thermophilum and Bifidobacterium pseudolongum strains: broth microdilution vs. agar disc diffusion assay.
There is urgent need for having available suitable methods and data regarding the susceptibility levels of antibiotic resistant and sensitive strains of bifidobacteria. Based on a defined standard operation procedure, agar disc diffusion and broth microdilution were compared in order to evaluate the antimicrobial susceptibility profiles of 82 B. pseudolongum and 80 B. thermophilum strains mainly originating from the meat production chain. The methods that were assessed showed interpretable agreement within this study. The disc diffusion zone diameters are highly reproducible making the method a useful alternative to broth microdilution for antimicrobial susceptibility screening of bifidobacteria.
Domig
Konrad J
KJ
BOKU-University of Natural Resources and Applied Life Sciences, Department of Food Science and Technology, Division of Food Microbiology and Hygiene, Gregor Mendel Str. 33, A-1180 Vienna, Austria. konrad.domig@boku.ac.at
Mayrhofer
Sigrid
S
Zitz
Ulrike
U
Mair
Christiane
C
Petersson
Agnes
A
Amtmann
Ernst
E
Mayer
Helmut K
HK
Kneifel
Wolfgang
W
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001644:Bifidobacterium / Q000187:drug effects* / Q000254:growth & development; D015169:Colony Count, Microbial / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial; D005516:Food Microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D019936:Probiotics
D000900:Anti-Bacterial Agents