title: [Identification of a new Puumala hantavirus subtype in rodents from China].
abstract: In order to detect the nucleic acid of Puumala hantavirus, RNA was extracted from lungs of bank voles captured in Northeast China, and partial S and M genome segments of Puumala virus were amplified by RT-PCR and sequenced. Phylogenetic analysis suggested that Chinese Puumala virus had diverged from the common node of PUUV, with accumulating nucleotide substitutions and formed a distinct lineage from other Puumala viruses. Newly found Puumala virus was most closely related to the Kamiiso-8Cr-95 and Tobetsu-60Cr-93 strains which came from Japan and the muju strains which came from South Korea. By analysis of S and M genome segments of Puumala virus, we deduced a new Puumala virus subtype did exist in Northeast China.
authors: State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China. tlhfree@hotmail.com
authors: Li-hua
authors: LH
authors: Tang
authors: Quan-fu
authors: QF
authors: Zhang
authors: Mei-hong
authors: MH
authors: Xiu
authors: Guang-wei
authors: GW
authors: Gu
authors: Shen
authors: S
authors: Bo
authors: Xian-da
authors: XD
authors: Yang
authors: Mi-fang
authors: MF
authors: Liang
authors: De-xin
authors: DX
authors: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D002522:Chlorocebus aethiops; D019143:Evolution, Molecular; D016679:Genome, Viral; D006476:Orthohantavirus / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D029262:Puumala virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012377:Rodentia / Q000821:virology*; D017422:Sequence Analysis, DNA; D014709:Vero Cells