Evaluation of postharvest-processed oysters by using PCR-based most-probable-number enumeration of Vibrio vulnificus bacteria.
Postharvest processing (PHP) is used to reduce levels of Vibrio vulnificus in oysters, but process validation is labor-intensive and expensive. Therefore, quantitative PCR was evaluated as a rapid confirmation method for most-probable-number enumeration (QPCR-MPN) of V. vulnificus bacteria in PHP oysters. QPCR-MPN showed excellent correlation (R(2) = 0.97) with standard MPN and increased assay sensitivity and efficiency.
Wright
Anita C
AC
University of Florida, Department of Food Science and Human Nutrition, P.O. Box 110370, Gainesville, FL 32611, USA. acw@ufl.edu
Garrido
Victor
V
Debuex
Georgia
G
Farrell-Evans
Melissa
M
Mudbidri
Archana A
AA
Otwell
W Steven
WS
D000818:Animals; D015169:Colony Count, Microbial; D005516:Food Microbiology; D010125:Ostreidae / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D041261:Vibrio vulnificus / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification