title: A reliable quantitative method for the analysis of phenolic compounds in Brazilian propolis by reverse phase high performance liquid chromatography.
abstract: A sensitive and reliable RP-HPLC method was developed using a C18 CLC-ODS (M) - 4.6x250 mm(2)column and gradient elution for the analysis of phenolic compounds in propolis raw material and its products. A procedure for the extraction of phenolic compounds using aqueous ethanol (90%) with the addition of veratraldehyde as the internal standard (IS) was developed allowing to quantify ten compounds: caffeic acid, coumaric acid, ferulic acid, cinnamic acid, aromadendrin-4'-methyl ether (AME), isosakuranetin, drupanin, artepellin C, baccharin, and 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran acid (DCBEN). The developed method gave good detection response and linearity in the range of 20.83-533.33 microg/mL.
authors: Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto da Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors: João P B
authors: JP
authors: de Sousa
authors: Paula C P
authors: PC
authors: Bueno
authors: Luiz E
authors: LE
authors: Gregório
authors: Ademar A
authors: AA
authors: da Silva Filho
authors: Niege A J C
authors: NA
authors: Furtado
authors: Márcia L
authors: ML
authors: de Sousa
authors: Jairo K
authors: JK
authors: Bastos
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010636:Phenols / Q000032:analysis*; D011429:Propolis / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D010636:Phenols; D011429:Propolis