[Detection of the virus of hepatitis E isolates and elucidation of the possibility of their replication in vitro].
Blood serum samples collected from patients with acute hepatitis symptoms admitted to Infectious Disease Hospitals of Novosibirsk, Barnaul, and Irkutsk were studied. The serum samples were tested for the IgM and IgG antibodies to HEV using ELISA. Seropositive samples were tested using RT-PCR for HEV RNA. Two HEV strains were isolated, and thus HEV infection was identified for West Siberia. One of this strains is classified as HEV genotype I; the other, as genotype III. Cell culturing of these strains in green monkey kidney (4647) cells showed an ability of HEV genotype I strain to cause persistent infection.
Chausov
E V
EV
Ternovoĭ
V A
VA
Kochneva
G V
GV
Sviatchenko
V A
VA
Sivolobova
G F
GF
Grazhdantseva
A A
AA
Kiselev
N N
NN
Sakharova
E G
EG
Netesov
S V
SV
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D005838:Genotype; D006508:Hepatitis Antibodies / Q000097:blood; D006509:Hepatitis B / Q000097:blood / Q000150:complications / Q000235:genetics / Q000378:metabolism; D016751:Hepatitis E / Q000097:blood / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D016752:Hepatitis E virus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D010802:Phylogeny; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012800:Siberia; D014776:Virus Cultivation; D014779:Virus Replication / Q000502:physiology*
D006508:Hepatitis Antibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012367:RNA, Viral