Induced expression of the transcription of tropomodulin 1 by Wnt5a and characterization of the tropomodulin 1 promoter.
Microarray analysis was carried out to identify novel downstream target genes regulated by non-canonical Wnt signaling. We found that Tmod1, known as an actin-capping protein, is up-regulated by Wnt5a, while gelsolin, known as an actin-severing protein, is down-regulated by Wnt5a. As expected from the roles of genes regulated by Wnt5a, L929 cells expressing Wnt5a show abnormal cell shape and a reduced migration rate. Cloning and analysis of the putative promoter show that two conserved sequences, one in the 5'-end of the first exon and the other in the intron next to the first exon, are necessary for the basal promoter activity.
Yun
Sangmoon
S
Department of Life Science, The University of Seoul, 90 Jeonnong-dong, Dongdaemun-gu, Seoul 130-743, Republic of Korea.
Rim
Yeonmi
Y
Jho
Eek-hoon
EH
D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D048430:Cell Shape / Q000235:genetics / Q000502:physiology; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D018260:Gelsolin / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014162:Transfection; D051348:Tropomodulin / Q000235:genetics*; D051153:Wnt Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D000071818:Wnt-5a Protein
D018260:Gelsolin; D011993:Recombinant Fusion Proteins; C495831:Tmod1 protein, mouse; D051348:Tropomodulin; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C438615:Wnt5a protein, mouse; D049452:Green Fluorescent Proteins; D008156:Luciferases