Elf-1 binds to GGAA elements on the FcRgamma promoter and represses its expression.
The Fc receptor (FcR) gamma-chain has been shown to be up-regulated in T cells when the TCR zeta-chain is decreased. We demonstrate that Elf-1, but not other Ets family transcription factors, bind to a cluster of GGAA sites located within the 200 bp upstream from the transcription initiation site of the FcRgamma promoter. Forced expression of Elf-1 results in the suppression of FcRgamma expression, whereas silencing its expression with small interfering RNA Elf-1 results in increased FcRgamma expression. Elf-1 represents the first transcription factor identified to be involved in the transcriptional regulation of FcRgamma, and cells that fail to express Elf-1, as is the case with human systemic lupus erythematosus T cells, will express FcRgamma-chain.
Juang
Yuang-Taung
YT
Department of Cellular Injury, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
Sumibcay
Laarni
L
Tolnay
Mate
M
Wang
Ying
Y
Kyttaris
Vasileios C
VC
Tsokos
George C
GC
D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D015536:Down-Regulation; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D050783:Proto-Oncogene Proteins c-ets / Q000378:metabolism*; D017452:Receptors, IgG / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*
D050783:Proto-Oncogene Proteins c-ets; D017452:Receptors, IgG; D014157:Transcription Factors