title: The effect of supplementing sow with bioactive substances on neonatal small intestinal epithelium.
abstract: Development of the small intestinal epithelium in early postnatal period has a significant influence on pig's survival rate and further productivity. The aim of this research was to verify whether the diet supplementation of pregnant and lactating sow with a blend of bioactive substances (flax seed, rapeseed, linden inflorescence, taurine, L-carnitine and tocopherol acetate) had an effect on the development of intestinal epithelium in their offspring. The doses of bioactive substances were calculated to meet the demands for optimal supply of the pig fetuses and newborns. Pig neonates from two groups of sows, control and supplemented, were sacrificed at the day 1, 2, 4, 7 and 14 of life. The samples taken from mid-jejunum were evaluated for mitosis (Ki67), apoptosis (active caspase 3), autophagy (MAP I LC3), and DNA damage (p53). Increase of mitotic index was noticed at day 1, 4 and 7 for supplemented group when compared to the control. Reduction of apoptotic index was observed at day 2 as compared to control. A tendency toward elevated autophagy was observed during the first 2-4 postnatal days in both groups. p53 expression was significantly lower in supplemented group as compared to control. Overall, the mitosis to programmed cell death ratio was increased and the maturation of epithelial cells quickened. We suppose that the supplementation of pregnant and lactating sow diet with bioactive substances enhanced maturation of the small intestinal epithelium in their offspring during the early postnatal period.
authors: Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw Agricultural University, Warsaw, Poland.
authors: A K
authors: AK
authors: Strzałkowski
authors: M M
authors: MM
authors: Godlewski
authors: N
authors: N
authors: Hallay
authors: G
authors: G
authors: Kulasek
authors: Z
authors: Z
authors: Gajewski
authors: R
authors: R
authors: Zabielski
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects; D001343:Autophagy / Q000187:drug effects; D029741:Brassica rapa / Q000737:chemistry; D002331:Carnitine / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects; D019587:Dietary Supplements; D005260:Female; D019597:Flax / Q000737:chemistry; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D007583:Jejunum / Q000187:drug effects* / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects; D013552:Swine; D013654:Taurine / Q000494:pharmacology; D032384:Tilia / Q000737:chemistry; D024505:Tocopherols; D024502:alpha-Tocopherol / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D013654:Taurine; D024502:alpha-Tocopherol; D024505:Tocopherols; D002331:Carnitine