Sensitive spectrofluorimetric determination of cytochrome C with spirocyclic rhodamine B hydrazide in micellar medium.
A new spectrofluorimetric method for the determination of cytochrome c using spirocyclic rhodamine B hydrazide (RBH) as fluorogenic reagent in the presence of sodium dodecylbenzene sulfonate (SDBS) surfactant micelles was developed. The method was based on the reaction of cytochrome c with RBH, a colorless, nonfluorescent spirolactam of rhodamine B in SDBS micelles to give highly fluorescent rhodamine B and hence led to a large increase in fluorescence intensity. The dynamic range and detection limit for the determination of cytochrome c are 4.0-120 ng ml(-1) and 0.87 ng ml(-1) (3sigma), respectively. The optimal conditions for the detection of cytochrome c were evaluated and the possible detection mechanism was also discussed.
Shen
Yehua
Y
Department of Chemistry, Northwest University, Xi'an 710069, China. Yhshen@nwu.edu.cn
Yang
Xiao-Feng
XF
Wu
Yao
Y
Li
Cong
C
D045304:Cytochromes c / Q000032:analysis* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D008823:Micelles; D012235:Rhodamines / Q000032:analysis* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D008823:Micelles; D012235:Rhodamines; D045304:Cytochromes c; C029773:rhodamine B