[Role of AT2 receptors on angiotensin II-induced tumor necrosis factor alpha and interleukin 1 beta secretion in adult rat cardiac fibroblasts].
OBJECTIVE
To investigate the role of AT2 receptors in the secretion of tumor necrosis factor alpha (alpha-TNF) and interleukin 1 beta (IL1 beta) in adult rat cardiac fibroblasts.


METHODS
Adult rat cardiac fibroblasts in in vitro culture were divided into control, Ang II, AngII + Losartan, and AngII + PD123319 groups with corresponding treatments. Radioimmunoassay was used to determine alpha-TNF and IL1 beta levels in the supernatant of the treated cardiac fibroblasts.


RESULTS
Ang II treatment resulted in significantly increased alpha-TNF and IL1 beta levels. Compared with AngII group, IL1 beta level was decreased by 69.1% and 78.7% and alpha-TNF by 58.7% and 65.9% after blocking AT1 and AT2 receptors, respectively.


CONCLUSIONS
AT2 receptors are involved in alpha-TNF and IL1 beta secretions in cardiac fibroblasts.
Jiang
Xiao-ying
XY
Department of Physiology and Pathophysiology, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
Gao
Guang-dao
GD
Zhou
Juan
J
Guo
Rong
R
Li
Yuan-xi
YX
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D053583:Interleukin-1beta / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044139:Receptor, Angiotensin, Type 2 / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D053583:Interleukin-1beta; D044139:Receptor, Angiotensin, Type 2; D014409:Tumor Necrosis Factor-alpha; D000804:Angiotensin II