Embryonic cells depleted of beta-catenin remain competent to differentiate into dorsal mesodermal derivatives.
Disruption of axis specification leads to defects in dorsal tissue patterning and cell movements. Here, we examine how beta-catenin coordinately affects gastrulation movements and dorsal mesoderm differentiation. The reduction of beta-catenin protein levels by morpholino oligonucleotides complementary to beta-catenin mRNA causes a disruption in gastrulation movements. Time-lapse imaging of beta-catenin morphants during gastrulation reveals that involution occurs simultaneously around the blastopore in the absence of convergent extension cell movements. Transplantation experiments show that morphant cells grafted from the marginal zone into wild-type hosts differentiate into notochord and muscle. However, wild-type mesoderm cells grafted to the marginal zone of beta-catenin morphants do not form dorsal tissues. These data argue that beta-catenin is not required for the initial establishment of dorsal mesoderm cell competency, but it is required for the maintenance of that competency. We propose that tissue interactions that occur during convergent extension movements are necessary for maintaining dorsal tissue competency.
Chu
Francie H
FH
Department of Biology, San Francisco State University, San Francisco, California 94132, USA.
Afonin
Bonnie
B
Gustin
Jean K
JK
Bost
Alyssa
A
Sanchez
Michael
M
Domingo
Carmen R
CR
D000818:Animals; D002454:Cell Differentiation; D004625:Embryo, Nonmammalian / Q000166:cytology*; D054262:Gastrulation; D018507:Gene Expression Regulation, Developmental; D008648:Mesoderm / Q000166:cytology / Q000196:embryology*; D009024:Morphogenesis; D032342:Muscle Cells / Q000166:cytology; D009672:Notochord / Q000166:cytology / Q000196:embryology; D009841:Oligonucleotides; D014982:Xenopus laevis; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
D009841:Oligonucleotides; D051176:beta Catenin