Entrainment of Na/K pumps by a synchronization modulation electric field.
We studied entrainment of the catalytic cycle of the Na/K pumps by an imposed external AC electric field. Our results show that a well designed dichotomous oscillating electric field with a frequency close to the pumps' natural turnover rate can synchronize the pump molecules. Characteristics of the synchronized pumps include: (1) outward pump currents responding to Na-extrusion and inward pump currents responding to K-pumping in are separated; (2) magnitude of the outward pump currents can be up to three times higher than that of the randomly paced pump currents; (3) magnitude ratio of the outward over inward pump currents reveals the 3:2 stoichiometry of the pumps. We, further, gradually increased the field oscillating frequency in a stepwise pattern and kept pump synchronization in each step. We found that the pumps' turnover rate could be modulated up as the field frequency increased. Consequently, the pump currents significantly increased by many fold. In summary, these results show that the catalytic cycle of Na/K pumps can be synchronized and modulated by a well designed oscillating electric field resulting in activation of the pump functions.
Chen
Wei
W
Cellular and Molecular Biophysics, University of South Florida, 4202 E. Fowler Avenue, Tampa, FL 33620, USA. wchen@cas.usf.edu
Zhang
Zhongsheng
Z
Huang
Feiran
F
D000818:Animals; D004558:Electric Stimulation; D004594:Electrophysiology; D066298:In Vitro Techniques; D007700:Kinetics; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D011894:Rana pipiens / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D000254:Sodium-Potassium-Exchanging ATPase