title: Analysis of the canine mdr1-1Delta mutation in the dog breed Elo.
abstract: A deletion mutation in the canine multidrug resistance gene, MDR1, is associated with drug sensitivity. This was shown for several purebred dog breeds from the Collie lineage such as the Collie (rough-coated and smooth-coated), the Australian Shepherd and the Old English sheepdog. To determine whether the mdr1-1Delta mutation could be found in the newly bred German dog breed Elo which is based amongst other breeds on Old English sheepdogs, 177 blood samples representative for the Elo breed were collected. After DNA extraction, a polymerase chain reaction-based method with subsequent polyacrylamide gel electrophoresis was used for detection of the mdr1-1Delta mutation. The mdr1-1Delta allele was not observed in the Elos investigated. The probability that the mdr1-1Delta allele originated in the Old English sheepdog breed is segregating in the Elo population was estimated at 3.68 x 10(-17).
authors: Institute of Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Bünteweg 17p, 30559 Hannover, Germany.
authors: S
authors: S
authors: Fecht
authors: A
authors: A
authors: Wöhlke
authors: H
authors: H
authors: Hamann
authors: O
authors: O
authors: Distl
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics*; D000483:Alleles; D000818:Animals; D001947:Breeding; D004283:Dog Diseases / Q000188:drug therapy / Q000235:genetics; D004285:Dogs / Q000235:genetics*; D018432:Drug Resistance, Multiple / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D019450:Genes, MDR / Q000235:genetics*; D005838:Genotype; D008297:Male; D010802:Phylogeny; D017384:Sequence Deletion
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1