title: [Effects of fluid shear stress strength on mRNA expression of ATP6V1a1 in polarized osteoclasts].
abstract: OBJECTIVE
  To observe effects of fluid shear stress strength on mRNA expression of ATP6V1a1 in rat polarized osteoclasts.
  
  
  METHODS
  Rat polarized osteoclasts suffered 0.0 (control group), 0.9, 2.9, 8.7 and 26.3 dynes/cm2 fluid shear stress for 30 min. mRNA expression of ATP6V1a1 was detected by Real-Time fluorescent quantitation PCR.
  
  
  RESULTS
  mRNA expression of ATP6V1a1 in 0.0 (control group), 0.9, 2.9, 8.7 and 26.3 dynes/cm2 groups was (1.14 +/- 0.06) x 10(6), (1.62 +/- 0.09) x 10(6), (2.28 +/- 0.13) x 10(6), (3.24 +/- 0.18) x 10(6), (9.16 +/- 0.53) x 10(6) copy numbers, respectively (P < 0.05).
  
  
  CONCLUSIONS
  In the present study, polarized osteoclasts are sensitive to fluid shear stress. mRNA expression of ATP6V1a1 has increscent tendency along with increasing of fluid shear stress strength.
authors: Dept. of Prosthodontics, The Affiliated Stomatology Hospital of Medical College, Zhejiang University, Hangzhou 310006, China.
authors: Qing-hong
authors: QH
authors: Zhang
authors: Xing
authors: X
authors: Liang
authors: Meng-tao
authors: MT
authors: Liu
authors: Bao-min
authors: BM
authors: Zhu
authors: Jun
authors: J
authors: Fu
mesh_terms: D000818:Animals; D010010:Osteoclasts; D012333:RNA, Messenger; D051381:Rats; D033081:Shear Strength; D013314:Stress, Mechanical
chemical_list: D012333:RNA, Messenger