Design and performance of a 16S rRNA-targeted oligonucleotide probe for detection of members of the genus Bdellovibrio by fluorescence in situ hybridization.
A 16S rRNA-targeted, Cy3-labeled oligonucleotide probe was designed to detect members of the genus Bdellovibrio by fluorescence in situ hybridization. Specific hybridization conditions were established; however, the detection of bdellovibrios in environmental samples required enrichment, confirming that Bdellovibrio spp. are not present in large numbers in the environment.
Mahmoud
Khaled K
KK
Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada N6A 5C1.
McNeely
Damian
D
Elwood
Chelsea
C
Koval
Susan F
SF
D001501:Bdellovibrio / Q000235:genetics* / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S