Studies on the anti-tumor activities of the soy isoflavone daidzein on murine neuroblastoma cells.
Daidzein (4',7-dihydroxyisoflavone) and genistein (4',5,7-trihydroxyisoflavone) are two major isoflavones found predominantly in soy beans, as well as in certain traditional Chinese medicinal herbs and tea leaves. In the past decade, there have been extensive studies on the anti-tumor effects of genistein on cancers of the breast, prostate and colon in humans. However, the anti-tumor effects of daidzein on neuronal cancer cells and its action mechanisms remain poorly understood. In this study, daidzein was shown to inhibit the proliferation of a number of murine and human neuroblastoma cell lines in vitro. Using the murine neuroblastoma Neuro-2a (BU-1) cells as the cell model, daidzein was also found to prevent the cell cycle progression to G2/M phase and induced apoptosis of the neuronal tumor cells, as measured by flow cytometry and gel electrophoresis for fragmented DNA respectively. Taken together, our results showed that daidzein could exert pleiotropic effects on the murine neuroblastoma cells, including inhibition of cell proliferation, modulation of cell cycle check point regulation, and triggering of neuronal cell apoptosis.
Lo
F H
FH
Department of Biochemistry and the Food and Nutritional Sciences Programme, The Chinese University of Hong Kong, Shatin, Hong Kong, China. lofaihang@cuhk.edu.hk
Mak
N K
NK
Leung
K N
KN
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic; D017209:Apoptosis / Q000187:drug effects; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D005434:Flow Cytometry; D007202:Indicators and Reagents; D007529:Isoflavones / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D009447:Neuroblastoma / Q000188:drug therapy* / Q000473:pathology; D013778:Tetrazolium Salts; D013844:Thiazoles
D000972:Antineoplastic Agents, Phytogenic; D004968:Estrogens, Non-Steroidal; D007202:Indicators and Reagents; D007529:Isoflavones; D013778:Tetrazolium Salts; D013844:Thiazoles; C004742:daidzein; C022616:thiazolyl blue