Analysis of multiple enteric viral targets as sewage markers in coral reefs.
Water and coral mucus samples were collected from throughout the Florida Keys National Marine Sanctuary and the Dry Tortugas for three years and were analyzed for human enteric viruses (enteroviruses, noroviruses, hepatitis A virus and adenoviruses) as conservative markers of human sewage using molecular methods. Of the 100 coral and water samples collected, 40 contained genetic material from one or more human enteric viruses. DNA-based adenoviruses were detected widely, in 37.8% of samples and at 91% of stations, including 'pristine' reefs in the Dry Tortugas; however, the detection rate was < or =12% for the RNA-based enteroviruses and noroviruses (hepatitis A virus was never detected). The disparity between the prevalence of RNA- and DNA-based viruses suggests the need for additional work to determine the utility of adenovirus as marker of human sewage.
Lipp
Erin K
EK
The University of Georgia, Department of Environmental Health Science, 206 Environmental Health Science Bldg., Athens, GA 30602, USA. elipp@uga.edu
Futch
J Carrie
JC
Griffin
Dale W
DW
D000818:Animals; D037421:Anthozoa / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods*; D005431:Florida; D006801:Humans; D011156:Population Density; D012367:RNA, Viral / Q000032:analysis; D012623:Seawater / Q000821:virology*; D012722:Sewage; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification*
D004279:DNA, Viral; D012367:RNA, Viral; D012722:Sewage