Possible role of transforming growth factor beta and interleukin-4 in the up-regulation of CLC-2 and CLC-3 in chronic rhinosinusitis.
BACKGROUND
Chronic rhinosinusitis (CRS) may cover different disease entities, and the pathogenic mechanism remains unclear.


METHODS
The aim of this study was to evaluate the expression of chloride channel protein CLC-2 and CLC-3 in CRS without nasal polyps (CRSsNP) and evaluate the roles of interleukin (IL)-4 and transforming growth factor (TGF) beta in the up-regulation of CLC-2 and CLC-3. We detected expression of CLC-2 and CLC-3 in 17 patients with CRSsNP by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR), and we examined the concentration of TGF-beta, IL-4, IL-5, and interferon (IFN) gamma in ethmoid sinus mucosa by enzyme-linked immunosorbent assay (ELISA).


RESULTS
We found that CLC-2 and CLC-3 is up-regulated in CRSnNP and located in submucosal glands and epithelium of the ethmoid sinus. CLC-2 and CLC-3 mRNA correlated with IL-4 in CRSsNP (r = 0.57 and 0.65; p < 0.05). CLC-2 and CLC-3 mRNA correlated negatively with mucosal TGF-beta in CRSsNP (r = -0.49 and -0.54; p < 0.05).


CONCLUSIONS
We concluded that CLC-2 and CLC-3 is up-regulated in ethmoid mucosa and may affect the development of CRSsNP. TGF-beta and IL-4 may modulate the expression of CLC-2 and CLC-3 in CRSsNP.
Li
Huabin
H
Otorhinolaryngology Hospital of The First Affiliated Hospital of Sun Yat-sen University, and Otorhinolaryngology Institute of Sun Yat-sen University, Guangzhou, China.
Jiang
Hongyan
H
Cheng
Lei
L
Oh
Yun
Y
Xu
Geng
G
D000328:Adult; D000090007:CLC-2 Chloride Channels; D018118:Chloride Channels / Q000378:metabolism / Q000502:physiology*; D002908:Chronic Disease; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015847:Interleukin-4 / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012220:Rhinitis / Q000378:metabolism / Q000503:physiopathology*; D012852:Sinusitis / Q000378:metabolism / Q000503:physiopathology*; D016212:Transforming Growth Factor beta / Q000502:physiology*; D015854:Up-Regulation / Q000502:physiology*
D000090007:CLC-2 Chloride Channels; D018118:Chloride Channels; C115376:ClC-3 channel; D016212:Transforming Growth Factor beta; D015847:Interleukin-4