title: Overexpression, purification and characterization of a recombinant secretary catalase from Bacillus subtilis.
abstract: A recombinant Bacillus subtilis strain (KN25) was generated for the large-scale preparation of catalase. The B. subtilis katA gene encoding for catalase was cloned into the shuttle vector PRB374, downstream of the constitutively active vegII promoter, followed by transformation of the B. subtilis strain WB600 with the plasmid. The transformant strain, KN25 secretes high levels (3,500 U/ml) of catalase, which facilitates its purification. Three simple purification steps yielded nearly homogeneous catalase, with approximately 70% recovery. The purified recombinant catalase has a specific activity of 34,600 U/mg under optimal conditions, and is more resistant to acidic conditions than bovine liver catalase.
authors: Department of Drug Biosynthesis, School of Pharmacy, Fudan University, Shanghai 200032, PR China.
authors: Xunlong
authors: X
authors: Shi
authors: Meiqing
authors: M
authors: Feng
authors: Yujie
authors: Y
authors: Zhao
authors: Xin
authors: X
authors: Guo
authors: Pei
authors: P
authors: Zhou
mesh_terms: D001412:Bacillus subtilis / Q000502:physiology*; D002374:Catalase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation; D004795:Enzyme Stability; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D002374:Catalase