Identification of B cell epitopes at the C-terminus of latency-associated nuclear protein of the kaposi's sarcoma-associated herpesvirus.
The latency-associated nuclear antigen (LANA) of Kaposi's sarcoma-associated herpesvirus (KSHV) plays a key role in the induction of cell transformation, maintenance of viral episome, and modulation of immune response in human. To identify the presence of B cell epitopes within C-terminus of LANA and to characterize the monoclonal antibodies (MAbs) against this protein, we expressed the C-terminal region at aa 794-1000 of LANA (pLANA-C) in Escherichia coli as a fusion protein. KSHV-positive human sera were able to recognize the recombinant LANA-C in the Western blot analysis and ELISA. Mapping of antigenic epitopes of pLANA-C by KSHV-positive human sera revealed two B cell antigenic epitopes located at aa 846-854 and aa 794-822. The MAb 3F11 recognized a region between at aa 840 to 846 of LANA and exhibited a strong and specific binding to both pLANA-C and native viral LANA. These findings showed that pLANA-C and MAb 3F11 could be used for the detection of KSHV antibodies in human sera and for the advanced study of biological functions of LANA.
Yao
S
S
Key Laboratory of Pathogen Biology of Jiangsu Province, Nanjing Medical University, Nanjing 210029, P. R. China.
Tang
Q
Q
Cheng
L
L
Zeng
Y
Y
Chen
X
X
Qin
D
D
Lv
Z
Z
Lu
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000956:Antigens, Viral / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry* / Q000276:immunology; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000276:immunology / Q000821:virology; D019288:Herpesvirus 8, Human / Q000276:immunology*; D006801:Humans; D051379:Mice; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012514:Sarcoma, Kaposi / Q000175:diagnosis / Q000276:immunology / Q000821:virology; D017735:Virus Latency
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D018985:Epitopes, B-Lymphocyte; D009687:Nuclear Proteins; D011994:Recombinant Proteins; C109540:latency-associated nuclear antigen