Expression and significance of TWIST basic helix-loop-helix protein over-expression in gastric cancer.
OBJECTIVE
TWIST protein has been implicated in neoplastic transformation and development of some cancers. In this study, we aimed to investigate the expression of TWIST in gastric cancer and its clinical significance.


METHODS
A total of 76 cases of archival gastric cancer tissues were immunohistochemically evaluated for TWIST expression, and its expression was correlated with clinicopathological parameters. Semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the mRNA of TWIST in four gastric cancer cell lines and a normal immortalised gastric epithelial cell line (GES-1). The expression of TWIST protein in these cell lines and 14 pairs of fresh gastric carcinoma and adjacent normal tissue samples was detected by Western blotting.


RESULTS
TWIST expression increased in diffuse-type gastric carcinoma compared with intestinal-type gastric carcinoma (26/42, 61.9% versus 9/34, 26.5%, p<0.05). TWIST expression was significantly increased in 35 (46.1%) of the 76 cancers and correlated with lymph node metastasis (node positive rate 60.4%; node negative rate 21.4%; p<0.05). The expression of TWIST protein was higher in 9/14 (64.3%) fresh cancer tissues compared with adjacent normal tissues. The expression of mRNA and protein of TWIST in gastric cancer cell lines was up-regulated compared with that in GES-1.


CONCLUSIONS
TWIST was highly expressed in gastric cancer. Its up-regulation was associated with the neoplastic transformation and subsequent development of gastric cancer. Therefore, TWIST may be a useful prognostic marker and target for gastric cancer therapy.
Yan-Qi
Zhang
Z
State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, the Fourth Military Medical University, 17 The West Road, Xi'an, Shaanxi Province 710032, China.
Xue-Yan
Guo
G
Shuang
Han
H
Yu
Chen
C
Fu-Lin
Ge
G
Fei-Hu
Bai
B
Shi-Ren
Sun
S
Xu-Feng
Wei
W
Jie
Ding
D
Dai-Ming
Fan
F
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008679:Metaplasia / Q000378:metabolism; D008875:Middle Aged; D009687:Nuclear Proteins / Q000096:biosynthesis*; D011230:Precancerous Conditions / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D051879:Twist-Related Protein 1 / Q000096:biosynthesis*; D015854:Up-Regulation
D009687:Nuclear Proteins; D012333:RNA, Messenger; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1