Surfactant-enhanced anaerobic acidogenesis of Canna indica L. by rumen cultures.
Polyoxyethylene sorbitan monoolate (Tween 80) was used to enhance the anaerobic acidogenesis of Canna indica L. (canna) by rumen culture in this study. Dose of Tween 80 at 1 ml/l enhanced the volatile fatty acids (VFA) production from the acidogenesis of canna compared to the control. However, Tween 80 at higher dosages than 5 ml/l inhibited the rumen microbial activity and reduced the VFA yield. Response surface methodology was successfully used to optimize the VFA yield. A maximum of VFA yield of 0.147 g/g total solids (TS) added was obtained at canna and Tween 80 concentrations of 6.3g TS/l and 2.0 ml/l, respectively. Dosage of Tween 80 at 1-3.75 ml/l reduced the unproductive adsorption of microbes or enzymes on the lignin part in canna and increased microbial activity. A high VFA production was achieved from canna presoaked with Tween 80, suggesting that the structure of canna was disrupted by Tween 80.
Yue
Zheng-Bo
ZB
Laboratory of Environmental Biotechnology, School of Chemistry, University of Science and Technology of China, Hefei 230026, China.
Yu
Han-Qing
HQ
Hu
Zhen-Hu
ZH
Harada
Hideki
H
Li
Yu-You
YY
D000327:Adsorption / Q000187:drug effects; D000693:Anaerobiosis / Q000187:drug effects; D000818:Animals; D001673:Biodegradation, Environmental / Q000187:drug effects; D027581:Cannabaceae / Q000187:drug effects* / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000032:analysis; D011136:Polysorbates / Q000494:pharmacology*; D012044:Regression Analysis; D012417:Rumen / Q000187:drug effects* / Q000201:enzymology / Q000382:microbiology*; D013379:Substrate Specificity / Q000187:drug effects; D013501:Surface-Active Agents / Q000494:pharmacology*
D005232:Fatty Acids, Volatile; D011136:Polysorbates; D013501:Surface-Active Agents; D002480:Cellulase; C019826:carboxymethylcellulase