One-step purification of epigallocatechin gallate from crude green tea extracts by mixed-mode adsorption chromatography on highly cross-linked agarose media.
(-)-Epigallocatechin gallate (EGCG) was purified in one step from a green tea polyphenol (GTP) crude extract by adsorption chromatography on a Superose 12 HR 10/30 column. The mobile phase used was a mixture of acetonitrile and water with an optimum mobile phase compositions regarding purity, recovery and separation time of 78/22 (v/v). Maximum practical sample loading was 100 mg GTP per run (corresponding to 4.2 mg/ml Superose). An EGCG purity of 99% with recoveries in the range 60-65% was achieved in one step directly from the crude GTP extract. Full column regeneration was obtained using solvents in the following order: 0.5 M NaOH, distilled water and 30% acetic acid.
Xu
Jun
J
Department of Bioengineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Tan
Tianwei
T
Janson
Jan-Christer
JC
D000097:Acetonitriles / Q000737:chemistry; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D005591:Chemical Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D012685:Sepharose / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013662:Tea / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D010936:Plant Extracts; D012997:Solvents; D013662:Tea; D014867:Water; D002392:Catechin; D012685:Sepharose; C049225:Superose 12; C045651:epigallocatechin gallate; C032159:acetonitrile