title: Characterization of discriminant human brain antigenic targets in neuropsychiatric systemic lupus erythematosus using an immunoproteomic approach.
abstract: OBJECTIVE
  To characterize discriminant human brain antigenic targets in patients with neuropsychiatric systemic lupus erythematosus (NPSLE), using a standardized immunoproteomic approach.
  
  
  METHODS
  Self-IgG reactivity against normal and injured human brain tissues was studied by Western blotting of sera from 169 subjects, 16 patients with NPSLE, 12 patients with SLE without neuropsychiatric manifestations (non-NPSLE), 32 patients with Sjögren's syndrome with or without central nervous involvement, 82 patients with multiple sclerosis, and 27 healthy subjects. A proteomic approach was then applied to characterize discriminant antigens identified after comparisons of all patterns.
  
  
  RESULTS
  The serum self-IgG reactivity patterns against human brain tissue differed significantly between patients with NPSLE and the control groups. Four normal brain antigenic bands were specifically or preferentially recognized by sera from NPSLE patients (p240, p90, p77, and p24). Protein band p240 was characterized as microtubule-associated protein 2B (MAP-2B), p77 as Hsp70-71, and p24 as triosephosphate isomerase. Protein band p90 was not characterized. In contrast, 1 other protein band (p56, characterized as septin 7) was never recognized by sera from NPSLE patients but was recognized by a majority of sera from non-NPSLE patients.
  
  
  CONCLUSIONS
  Our findings show that the immunoproteomic approach is a reliable method for assessing serum self-IgG reactivities against human brain tissue in NPSLE. Our characterization of some of the identified discriminant antigens, such as MAP-2B, triosephosphate isomerase, and septin 7, suggests that the stability of neuronal microtubules might be involved in the pathophysiology of NPSLE.
authors: Laboratoire d'Immunologie EA 2686, IMPRT-IFR 114, Université Lille 2, Lille, France. didier-lefranc@wanadoo.fr
authors: Didier
authors: D
authors: Lefranc
authors: David
authors: D
authors: Launay
authors: Sylvain
authors: S
authors: Dubucquoi
authors: Jérôme
authors: J
authors: de Seze
authors: Patricia
authors: P
authors: Dussart
authors: Marie
authors: M
authors: Vermersch
authors: Eric
authors: E
authors: Hachulla
authors: Pierre-Yves
authors: PY
authors: Hatron
authors: Patrick
authors: P
authors: Vermersch
authors: Luc
authors: L
authors: Mouthon
authors: Lionel
authors: L
authors: Prin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D015153:Blotting, Western; D001921:Brain / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D020945:Lupus Vasculitis, Central Nervous System / Q000276:immunology*; D008297:Male; D008869:Microtubule-Associated Proteins / Q000276:immunology*; D008875:Middle Aged; D012859:Sjogren's Syndrome / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D007074:Immunoglobulin G; D008869:Microtubule-Associated Proteins