[Effect of daunorubicin and cytarabine on cell line NB4].
OBJECTIVE
To explore whether daunorubicin (DNR) combined with cytosine arabinoside (Ara-C) and DNR alone have similar effect on acute promyelocytic leukemia (APL) cell line NB4 and acute myeloblastic leukemia cell line HL-60 in vitro.


METHODS
Cell morphology, cells viability, and cell apoptosis (Annexin-V by flow cytometry assay) were analysed.


RESULTS
After incubation with DNR plus Ara-C for 24 hours,NB4 cell viability [(36.75 +/- 3.82)%] (n = 6) and cell apoptosis rate [(21.24 +/- 5.82)%] (n = 3) did not change significantly compared to that treated with DNR alone for 24 hours [(35.73 + 6.28 )%, (22.55 +/- 3.26)%, respectively] (P > 0.05). However, HL-60 cell viability [(67.17 +/- 2.07)%] and cell apoptosis rate [(48.05 +/- 0.92)%] changed significantly in DNR plus Ara-C group compared with DNR alone [(63.31 +/- 1.80)% ,(41.51 +/- 0.89)%, respectively] (P < 0.01 and < 0.05, respectively).


CONCLUSIONS
DNR plus Ara-C and DNR alone have similar effect on NB4 cells, but have different effect on HL-60 cells.
Luo
Zhong-Guang
ZG
Department of Hematology, Renji Hospital, Medical College of Shanghai Jiaotong University, Shanghai 200001, China.
Zhong
Lu
L
Chen
Fang-Yuan
FY
Wang
Hai-Rong
HR
Ouyang
Ren-Rong
RR
D017209:Apoptosis / Q000187:drug effects; D003561:Cytarabine / Q000494:pharmacology*; D003630:Daunorubicin / Q000494:pharmacology*; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*
D003561:Cytarabine; D003630:Daunorubicin