title: MTPA: a crustacean metallothionein that affects hepatopancreatic mitochondrial functions.
abstract: Metallothioneins are cysteine-rich proteins, with a high capacity to bind metallic ions, and for which a precise biological role has not been established. Here we investigated the effects of MTPA, a metallothionein from the lobster Panulirus argus, on mitochondrial oxygen consumption and ROS production. An HPLC-RP-ESI-MS analysis of recombinant MTPA showed that despite its extra Cys, MTPA binds 6 Zn2+ per molecule akin to other crustacean metallothioneins with 18 Cys. The extra Cys is not involved in zinc binding, since its side-chain would be oriented to the outside of the molecule according to a preliminary model of the tridimensional structure of MTPA. MTPA-Zn2+(6) is imported into the hepatopancreatic mitochondria intermembrane space and inhibits mitochondrial oxygen consumption, increasing thereby ROS production. Nevertheless, the stimulation of ROS production by MT-bound Zn2+ is weaker compared to equivalent amounts of free Zn2+, suggesting that MTPA protects against oxidative stress. This constitutes the first report on metallothioneins effects on mitochondrial function in invertebrates and agrees with the results described for mammals, suggesting a connection between metallothioneins and energy metabolism.
authors: Biochemistry Section, Faculty of Chemistry, Regional Centre for Biomedical Research (CRIB), University of Castilla-La Mancha, Camilo José Cela, 10, 13071 Ciudad Real, Spain.
authors: Eduardo
authors: E
authors: Moltó
authors: Elena
authors: E
authors: Bonzón-Kulichenko
authors: Nilda
authors: N
authors: Gallardo
authors: Antonio
authors: A
authors: Andrés
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003445:Crustacea; D008099:Liver / Q000502:physiology*; D008668:Metallothionein / Q000737:chemistry*; D008928:Mitochondria / Q000378:metabolism; D008958:Models, Molecular; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010101:Oxygen Consumption; D010179:Pancreas / Q000502:physiology*; D017382:Reactive Oxygen Species; D017386:Sequence Homology, Amino Acid; D021241:Spectrometry, Mass, Electrospray Ionization; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D008668:Metallothionein