Use of solid-supported liquid-liquid extraction in the analysis of polyphenols in wine.
Solid-supported liquid-liquid extraction (SS-LLE) was compared to liquid-liquid extraction (LLE) for the analysis of phenolic compounds in wine. Diatomaceous earth commercial cartridges were evaluated together with "in-house" made cartridges for the wine phenolic extraction. Statistical treatment, analysis of variance ANOVA-single factor, was used to compare the extraction yields obtained by these methods, and for the majority of the studied compounds, significantly higher yields were obtained by the SS-LLE methodology using the "in-house" prepared cartridges. This is an environmentally friendly low-cost sample preparation method which proved to be reproducible (RSD<5% for the most compounds) and yielding high recoveries (80-100%) for the compounds studied.
Nave
Frederico
F
Departamento de Química da Universidade de Evora, Rua Romão Ramalho no. 59, 7000-617 Evora, Portugal.
Cabrita
Maria João
MJ
da Costa
Cristina Teixeira
CT
D002138:Calibration; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007692:Diatomaceous Earth / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D005504:Food Analysis / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D059808:Polyphenols; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods; D014466:Ultraviolet Rays / Q000145:classification; D014920:Wine / Q000032:analysis* / Q000145:classification
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D007692:Diatomaceous Earth