[Molecular subtyping of Vibrio cholerae isolates by pulsed-field gel electrophoresis in Shenzhen from 1993].
OBJECTIVE
To determine the genetic relationships between different Vibrio cholerae isolates in Shenzhen from 1993 to 2002.


METHODS
Chromosomal DNA from 60 isolates was digested in seakem gold agrose with restriction enzyme Not I and plugs were then analyzed by pulsed-field gel electrophoresis. Pulsed-field gel electrophoresis (PFGE) patterns of V. cholerae isolates were clustered using BioNumerics software.


RESULTS
39 distinctive PFGE patterns were identified with each pattern having 20 to 30 bands. Most PFGE patterns were divided into cluster A or cluster B.


CONCLUSIONS
The closely related pandemic clone clusters of V. cholerae strains did exist in Shenzhen. PFGE of V. cholerae could be used for active surveillance and tracking for cholerae.
Lan
Quan-xue
QX
Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.
Hu
Qing-hua
QH
Shi
Xiao-lu
XL
Wang
Bing
B
Lin
Yi-man
YM
Zhang
Shun-xiang
SX
Cheng
Jin-quan
JQ
Kan
Biao
B
Xu
Jian-guo
JG
D002681:China / Q000453:epidemiology; D002771:Cholera / Q000453:epidemiology / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D010802:Phylogeny; D014734:Vibrio cholerae / Q000145:classification* / Q000235:genetics*