Structure determination of the O-methyltransferase NovP using the 'free lunch algorithm' as implemented in SHELXE.
NovP is an S-adenosyl-L-methionine-dependent O-methyltransferase from Streptomyces spheroides (subunit MW = 29 967 Da). Recombinant N-terminally His-tagged NovP crystallizes in space group P2, with approximate unit-cell parameters a = 51.81, b = 46.04, c = 61.22 A, beta = 105.0 degrees , giving a solvent content of 44% for a single copy of the His-tagged protomer per asymmetric unit. Native synchrotron data to a resolution of 1.35 A were combined with three other native data sets collected at lower resolution (both in-house and at the synchrotron) for the sake of completeness and better scaling. Data to 2.45 A resolution were subsequently recorded in-house from a single mercury derivative. Three partial mercury sites could be located with SHELXD, but the resulting phases had a mean error of about 81 degrees and in our hands did not yield an interpretable map using standard automated software. Nevertheless, the structure of NovP could be solved by first tracing a small part of the structure by hand and then extrapolating within and beyond the experimental resolution limit using the ;free lunch algorithm' in SHELXE. The resulting phases have a mean phase error of 17 degrees relative to a refined model.
Usón
Isabel
I
Institució Catalana de Recerca i Estudis Avançats at Instituto de Biología Molecular de Barcelona (IBMB-CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain. uson@ibmb.csic.es
Stevenson
Clare E M
CE
Lawson
David M
DM
Sheldrick
George M
GM
D000465:Algorithms; D001331:Automation; D018360:Crystallography, X-Ray / Q000379:methods; D005583:Fourier Analysis; D046911:Macromolecular Substances; D008780:Methyltransferases / Q000737:chemistry*; D015233:Models, Statistical; D008968:Molecular Conformation; D011487:Protein Conformation; D011497:Protein O-Methyltransferase / Q000737:chemistry*; D012984:Software; D013302:Streptomyces / Q000201:enzymology*; D017356:Synchrotrons
D046911:Macromolecular Substances; D008780:Methyltransferases; D011497:Protein O-Methyltransferase; C524783:NovP protein, Streptomyces