Detection of Aspergillus carbonarius and other black aspergilli from grapes by DNA OLISA microarray.
Black aspergilli, and particularly Aspergillus carbonarius, are responsible for ochratoxin A production in grapes. Correct identification of these species is essential for toxicological risk assessment in grape and wine. A low-complexity oligonucleotide microarray (OLISA, Apibio, F) based on DNA oligonucleotides probes, obtained from sequences of the calmodulin gene, was set up in order to detect A. carbonarius, A. japonicus/A. aculeatus and A. ibericus isolated from grape. The designed microarray distinguished all Aspergillus species and the detection limit for A. carbonarius was 3.2 pg of DNA as a template for the PCR reaction. This microarray offers a quick and parallel analysis to detect individual Aspergillus species in pure cultures and in naturally contaminated grape samples.
Bufflier
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BioMerieux-Molecular Biology Unit, Parc Polytec, 5 rue des Berges, F-38024 Grenoble cedex, France.
Susca
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A
Baud
M
M
Mulè
G
G
Brengel
K
K
Logrieco
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A
D001230:Aspergillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000235:genetics; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D015870:Gene Expression; D009793:Ochratoxins / Q000096:biosynthesis*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity; D027843:Vitis / Q000382:microbiology*
D004271:DNA, Fungal; D009793:Ochratoxins; C025589:ochratoxin A