Tissue microarrays in clinical urology--technical considerations.
The tissue microarray (TMA) technique is a 'high-throughput' tool which enables a rapid and concurrent analysis of molecular targets in large numbers of specimens at the DNA, RNA and protein levels under standardized conditions. In addition, TMA is a cost- and time-efficient method. This review focuses on its technical side, outlining its advantages and weaknesses. It also gives an overview of some major studies of bladder and prostate cancer in which the TMA technique was used.
Kramer
Mario W
MW
Department of Urology, Eberhard-Karls-University Tübingen, Tübingen, Germany. mariokramer@gmail.com
Merseburger
Axel S
AS
Hennenlotter
Jorg
J
Kuczyk
Markus
M
D005260:Female; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D040081:Protein Array Analysis; D046888:Tissue Array Analysis / Q000379:methods*; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism