Interaction between the keratin cytoskeleton and eEF1Bgamma affects protein synthesis in epithelial cells.
Eukaryotic elongation factor-1 (eEF1) is essential for peptide-chain elongation during translation. We report that its gamma subunit (eEF1Bgamma) specifically binds, and bundles, keratin intermediate filaments. Disrupting this interaction depresses translation by approximately 20% and selectively increases 80S ribosomes in epithelial cells, an outcome recapitulated by RNA interference-mediated silencing of eEF1Bgamma. These findings extend the emerging relationship between keratin proteins and the translational machinery.
Kim
Seyun
S
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, Maryland 21205, USA.
Kellner
Juliane
J
Lee
Chang-Hun
CH
Coulombe
Pierre A
PA
D000595:Amino Acid Sequence; D000818:Animals; D003599:Cytoskeleton / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007633:Keratins / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D016415:Sequence Alignment
D020648:Peptide Elongation Factor 1; D021122:Protein Subunits; D007633:Keratins