Metabolomics (liver and blood profiling) in a mouse model in response to fasting: a study of hepatic steatosis.
A metabolomic approach was applied to a mouse model of starvation-induced hepatic steatosis. After 24 h of fasting it appears that starvation reduced the phospholipids (PL), free cholesterol (FC), and cholesterol esters (CE) content of low-density lipoproteins (LDL). In liver lipid profiles major changes were observed using different techniques. High performance thin layer chromatography (HPTLC)-measurements of liver-homogenates indicated a significant rise of FC with 192%, triacylglycerols (TG) with 456% and cholesterol esters (CE) with 268% after 24 h of starvation in comparison with the control group. Reversed phase liquid chromatography coupled to mass spectrometry measurements (LC-MS) of liver homogenate indicated that the intensity of Phosphatidylcholine (PC) in the 24-h starvation group dropped to 90% of the value in the control group while the intensity of CE and TG increased to 157% and 331%, respectively, of the control group. Interestingly, a 49:4-TG with an odd number of C atoms appeared during starvation. This unique triacylglycerol has all characteristics of a biomarker for detection of hepatic steatosis. These observations indicate that in mammals liver lipid profiles are a dynamic system which are readily modulated by environmental factors like starvation.
van Ginneken
V
V
Department of Anatomy and Embryology, Leiden University Medical Center (LUMC) P.O. Box 9600, 2300 RC, Leiden, The Netherlands. V.J.T.van.Ginneken@biology.leidenuniv.nl
Verhey
E
E
Poelmann
R
R
Ramakers
R
R
van Dijk
K Willems
KW
Ham
Lisanne
L
Voshol
P
P
Havekes
L
L
Van Eck
M
M
van der Greef
J
J
D000818:Animals; D001769:Blood / Q000378:metabolism*; D002788:Cholesterol Esters / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000378:metabolism*; D005508:Food Deprivation; D008077:Lipoproteins, LDL / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
D002788:Cholesterol Esters; D008077:Lipoproteins, LDL; D010713:Phosphatidylcholines; D010743:Phospholipids; D014280:Triglycerides