Structural analysis of the ParR/parC plasmid partition complex.
Accurate DNA partition at cell division is vital to all living organisms. In bacteria, this process can involve partition loci, which are found on both chromosomes and plasmids. The initial step in Escherichia coli plasmid R1 partition involves the formation of a partition complex between the DNA-binding protein ParR and its cognate centromere site parC on the DNA. The partition complex is recognized by a second partition protein, the actin-like ATPase ParM, which forms filaments required for the active bidirectional movement of DNA replicates. Here, we present the 2.8 A crystal structure of ParR from E. coli plasmid pB171. ParR forms a tight dimer resembling a large family of dimeric ribbon-helix-helix (RHH)2 site-specific DNA-binding proteins. Crystallographic and electron microscopic data further indicate that ParR dimers assemble into a helix structure with DNA-binding sites facing outward. Genetic and biochemical experiments support a structural arrangement in which the centromere-like parC DNA is wrapped around a ParR protein scaffold. This structure holds implications for how ParM polymerization drives active DNA transport during plasmid partition.
Møller-Jensen
Jakob
J
MRC-Laboratory of Molecular Biology, Cambridge, UK. jakobm@bmb.sdu.dk
Ringgaard
Simon
S
Mercogliano
Christopher P
CP
Gerdes
Kenn
K
Löwe
Jan
J
D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D002503:Centromere / Q000648:ultrastructure; D018360:Crystallography, X-Ray / Q000379:methods; D004261:DNA Replication; D027101:DNA Topoisomerase IV / Q000378:metabolism*; D019281:Dimerization; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism; D008954:Models, Biological; D008958:Models, Molecular; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*
D000199:Actins; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C471255:ParM protein, E coli; C086169:ParR protein, bacteria; D012097:Repressor Proteins; D027101:DNA Topoisomerase IV