title: Role of copper in folding and stability of cupredoxin-like copper-carrier protein CopC.
abstract: CopC is a periplasmic copper carrier that, in contrast to cytoplasmic copper chaperones, has a beta-barrel fold and two metal-binding sites distinct for Cu(II) and Cu(I). The copper sites are located in each end of the molecule: the Cu(I) site involves His and Met coordination whereas the Cu(II) site consists of charged residues. To reveal biophysical properties of this protein, we have explored the effects of the cofactors on CopC unfolding in vitro. We demonstrate that Cu(II) coordination affects both protein stability and unfolding pathway, whereas Cu(I) has only a small effect on stability. Apo-CopC unfolds in a two-state reaction between pH 4 and 7.5 with maximal stability at pH 6. In contrast, Cu(II)-CopC unfolds in a three-state reaction at pH6 that involves a partly folded intermediate that retains Cu(II). This intermediate exhibits high thermal and chemical stability. Unique energetic and structural properties of different metalated CopC forms may help facilitate metal transport to many partners in vivo.
authors: Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street, Houston, TX 77251, USA.
authors: Faiza
authors: F
authors: Hussain
authors: Erik
authors: E
authors: Sedlak
authors: Pernilla
authors: P
authors: Wittung-Stafshede
mesh_terms: D001400:Azurin / Q000737:chemistry*; D001426:Bacterial Proteins / Q000378:metabolism; D001703:Biophysics / Q000379:methods; D002151:Calorimetry; D003300:Copper / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000737:chemistry; D008715:Methionine / Q000737:chemistry; D008958:Models, Molecular; D011489:Protein Denaturation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D021381:Protein Transport; D013816:Thermodynamics
chemical_list: D001426:Bacterial Proteins; C071265:CopC protein, Bacteria; D008670:Metals; C050107:cupredoxin; D001400:Azurin; D006639:Histidine; D003300:Copper; D008715:Methionine