title: Comparative proteomes of Corynebacterium glutamicum grown on aromatic compounds revealed novel proteins involved in aromatic degradation and a clear link between aromatic catabolism and gluconeogenesis via fructose-1,6-bisphosphatase.
abstract: The current study examined the aromatic degradation and central metabolism in Corynebacterium glutamicum by proteomic and molecular methods. Comparative analysis of proteomes from cells grown on gentisate and on glucose revealed that 30% of the proteins of which their abundance changed were involved in aromatic degradation and central carbon metabolism. Similar results were obtained from cells grown on benzoate, 4-cresol, phenol, and resorcinol. Results from these experiments revealed that (i) enzymes involved in degradation of benzoate, 4-cresol, gentisate, phenol, and resorcinol were specifically synthesized and (ii) that the abundance of enzymes involved in central carbon metabolism of glycolysis/gluconeogenesis, pentose phosphate pathway, and TCA cycles were significantly changed on various aromatic compounds. Significantly, three novel proteins, NCgl0524, NCgl0525, and NCgl0527, were identified on 4-cresol. The genes encoding NCgl0525 and NCgl0527 were confirmed to be necessary for assimilation of 4-cresol with C. glutamicum. The abundance of fructose-1,6-bisphosphatase (Fbp) was universally increased on all the tested aromatic compounds. This Fbp gene was disrupted and the mutant WT(Deltafbp) lost the ability to grow on aromatic compounds. Genetic complementation by the Fbp gene restored this ability. We concluded that gluconeogenesis is a necessary process for C. glutamicum growing on various aromatic compounds.
authors: State Key Laboratory of Microbial Resource, Institute of Microbiology, Chinese Academy of Sciences, Beijing, PR China.
authors: Su-Wei
authors: SW
authors: Qi
authors: Muhammad Tausif
authors: MT
authors: Chaudhry
authors: Yun
authors: Y
authors: Zhang
authors: Bo
authors: B
authors: Meng
authors: Yan
authors: Y
authors: Huang
authors: K-X
authors: KX
authors: Zhao
authors: Ansgar
authors: A
authors: Poetsch
authors: Cheng-Ying
authors: CY
authors: Jiang
authors: Siqi
authors: S
authors: Liu
authors: Shuang-Jiang
authors: SJ
authors: Liu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D048230:Corynebacterium glutamicum / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D006597:Fructose-Bisphosphatase / Q000502:physiology*; D005943:Gluconeogenesis / Q000502:physiology*; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006841:Hydrocarbons, Aromatic; D020543:Proteome; D006597:Fructose-Bisphosphatase