Fmoc-based solid phase chemical synthesis of 71-meric neuregulin 1-beta1, an epidermal growth factor-like domain.
The human neuregulin 1-beta1 (NRG1-beta1, amino acid residues 176-246) was chemically synthesized by Fmoc-based solid phase peptide synthesis (SPPS) followed by folding in a redox buffer. The biological activity of the synthesized NRG1-beta1 was confirmed by ligand-induced tyrosine phosphorylation on Chinese hamster ovary (CHO) cells expressing ErbB-4.
Kakizawa
Taeko
T
Department of Medicinal Chemistry, Center for Frontier Research in Medicinal Science, 21st Century COE Program, Kyoto Pharmaceutical University, Yamashina-ku, Kyoto 607-8412, Japan.
Koide-Yoshida
Shizuyo
S
Kimura
Tooru
T
Uchimura
Hiromasa
H
Hayashi
Yoshio
Y
Saito
Kazuki
K
Kiso
Yoshiaki
Y
D000596:Amino Acids / Q000737:chemistry*; D004815:Epidermal Growth Factor / Q000737:chemistry* / Q000502:physiology; D005449:Fluorenes / Q000737:chemistry*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D020890:Neuregulin-1; D010455:Peptides / Q000138:chemical synthesis*; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D017434:Protein Structure, Tertiary
D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; C094131:NRG1 protein, human; D009419:Nerve Tissue Proteins; D020890:Neuregulin-1; D010455:Peptides; D011108:Polymers; D004815:Epidermal Growth Factor