Detection of pediocin PA-1 in food matrices using specific polyclonal antibodies.
Pediocin PA-1 was conjugated with keyhole limpet hemocyanin (KLH) and used to immunize rabbits and mice for the production of polyclonal (PAb) and monoclonal (MAb) antibodies. Titers of PAb and MAb of about 4.7 and 2.9 were obtained after three and six immunizations, respectively. An enzyme linked immunosorbent assay (ELISA) was developed for the detection and quantification of pediocin.
Naghmouchi
K
K
STELA Dairy Research Center, Nutraceuticals and Functional Foods Institute (INAF), University Laval, Quebec, PQ, Canada, G1K 7P4.
Lacroix
C
C
Kheadr
E
E
Fliss
I
I
D000818:Animals; D000907:Antibodies, Bacterial; D001430:Bacteriocins / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005504:Food Analysis / Q000379:methods*; D005516:Food Microbiology; D051379:Mice; D000070017:Pediocins; D011817:Rabbits
D000907:Antibodies, Bacterial; D001430:Bacteriocins; D000070017:Pediocins; C074288:pediocin PA-1