Identification of IL-18RAP mRNA truncated splice variants in human testis and the other human tissues.
IL-18 is a pleiotropic cytokine involved in the regulation of both innate and adaptive immunity. It plays a key role in the autoimmune, inflammatory and infectious diseases. IL-18 acts via a receptor complex that closely resembles that of IL-1, consisting of a ligand binding protein, IL-18Ralpha, and an accessory protein, IL-18RAP (IL-18Rbeta). IL-18RAP is essential for IL-18 signal transduction and ligand binding affinity to IL-18Ralpha receptor chain. mRNA of gene coding for IL-18RAP in human testicular tissue and the nucleotide sequence of splice variants was carefully examined. We have found for the first time ever, IL-18RAP mRNA in studied tissue samples of physiological testis. Using the RT-PCR technique, the whole coding sequence of this gene was amplified. An alternative splicing of mRNA for IL-18RAP was then discovered and subsequently confirmed by cDNA sequencing. The putative amino acid content was predicted and a computer modeling was performed. It might be hypothesized that the truncated forms of IL-18RAP can be involved in the complex mechanism of IL-18 activity regulation.
Fiszer
Dorota
D
Institute of Human Genetics, Polish Academy of Sciences, Strzeszyńska 32, 60-479 Poznań, Poland. fiszdor@madn.poznan.pl
Rozwadowska
Natalia
N
Rychlewski
Leszek
L
Kosicki
Włodzimierz
W
Kurpisz
Maciej
M
D017398:Alternative Splicing / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D001483:Base Sequence; D003198:Computer Simulation; D006801:Humans; D020382:Interleukin-18 / Q000378:metabolism*; D053728:Interleukin-18 Receptor beta Subunit / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics / Q000276:immunology; D020033:Protein Isoforms / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D017384:Sequence Deletion; D013737:Testis / Q000166:cytology / Q000276:immunology / Q000378:metabolism*
D020382:Interleukin-18; D053728:Interleukin-18 Receptor beta Subunit; D020033:Protein Isoforms; D012333:RNA, Messenger