[Detection of differentially expressed genes in oral lichen planus].
OBJECTIVE
To detect the differentially expressed genes in oral lichen planus(OLP) by cDNA microarray.


METHODS
The PCR products of 4000 genes were spotted on chemical material coated glass plates in array. The total RNAs were isolated from OLP and normal oral mucosa tissue and were purified to mRNA. Both the mRNA were reversely transcribed to cDNA with the incorporations of fluorescent dUTP, for preparing the hybridization probes. The mixed probes were then hybridized to the cDNA microarray. Microarray was scanned for the fluorescent signals and showed the differences between the two samples.


RESULTS
The expressions of 122 genes were up regulated while the expressions of 91 genes were down regulated in OLP among the 4000 target genes. The up-regulated genes were mainly the ones of immunity related genes, metabolize related genes, oncogene, cytokine and cell signal transduction protein. The down-regulated genes were mainly the ones of DNA binding and transcription factors, cell signal transduction protein, immunity related genes, metabolize related genes, and cytokine.


CONCLUSIONS
Two hundred and thirteen differentially expressed genes with different functions were revealed in OLP, which may play some roles in the progression of OLP.
Fan
Yuan
Y
Research Institute of Stomatology, Nanjing Medical University, Nanjing 210029, China.
Zhan
Zhen
Z
Liu
Jie
J
D020869:Gene Expression Profiling; D006801:Humans; D017676:Lichen Planus, Oral; D009061:Mouth Mucosa; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger
D012333:RNA, Messenger