Prototype single step lateral flow technology for detection of avian influenza virus and chicken antibody to avian influenza virus.
A rapid and effective lateral flow assay (LFA) for detection of avian influenza virus (AIV) was developed. For antigen capture, the assay used monoclonal antibody specific for a conserved nuclear protein (NP) epitope, immobilized on a cellulose acetate matrix, in conjunction with a second NP monoclonal antibody chemically linked to either coloured latex beads or colloidal gold particles contained in a sample pad for detection. Virus sample added to the sample pad flowed into the trapping antibody to form a visible band as well as a second, control band further along the acetate strip. The control band consisted of recombinant protein A/G, also immobilized on the matrix. A second LFA for detection of chicken antibody to AIV was developed where NP antigen was immobilized on the matrix with recombinant protein A/G immobilized as a control band. Latex beads or colloidal gold particles to which monoclonal anti-chicken antibody was attached, were used as the indicator system.
Nielsen
K
K
Canadian Food Inspection Agency, Ottawa Laboratories (Fallowfield), 3851 Fallowfield Road, Ottawa, Ontario, Canada. nielsenk@inspection.gc.ca
Yu
W L
WL
Lin
M
M
Davis
S A Nadin
SA
Elmgren
C
C
Mackenzie
R
R
Tanha
J
J
Li
S
S
Dubuc
G
G
Brown
E G
EG
Keleta
L
L
Pasick
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D002645:Chickens / Q000276:immunology*; D017979:Gold Colloid; D007118:Immunoassay / Q000379:methods*; D009980:Influenza A virus / Q000276:immunology* / Q000302:isolation & purification*; D007252:Influenza Vaccines / Q000276:immunology; D005585:Influenza in Birds / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D008863:Microspheres; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D017979:Gold Colloid; D007252:Influenza Vaccines; D011994:Recombinant Proteins