Strong ion-exchange centrifugal partition chromatography as an efficient method for the large-scale purification of glucosinolates.
The glucosinolates sinalbin and glucoraphanin were purified by strong ion-exchange displacement centrifugal partition chromatography (SIXCPC). The optimized conditions involved the biphasic solvent system ethyl acetate/n-butanol/water (3:2:5, v/v), the lipophilic anion-exchanger Aliquat 336 (trioctylmethylammonium chloride, 160 and 408 mM) and a sodium iodide solution (80 and 272 mM) as displacer. Amounts as high as 2.4 g of sinalbin and 2.6g of glucoraphanin were obtained in one step in 2.5 and 3.5h respectively, starting from 12 and 25 g of mustard and broccoli seed aqueous extracts, using a laboratory scale CPC column (200 mL inner volume).
Toribio
Alix
A
FRE CNRS 2715, IFR 53 Biomolecules, UFR Pharmacie, Université de Reims Champagne-Ardenne, Bât. 18, Moulin de Housse, B.P. 1039, 51687 Reims Cedex 2, France.
Nuzillard
Jean-Marc
JM
Renault
Jean-Hugues
JH
D002498:Centrifugation / Q000379:methods*; D005591:Chemical Fractionation / Q000379:methods; D002794:Choline / Q000031:analogs & derivatives* / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange / Q000379:methods*; D005961:Glucosinolates / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007096:Imidoesters / Q000302:isolation & purification*; D015394:Molecular Structure; D009149:Mustard Plant / Q000737:chemistry; D010091:Oximes; D010936:Plant Extracts / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013454:Sulfoxides; D014867:Water / Q000737:chemistry
D005961:Glucosinolates; D007096:Imidoesters; D010091:Oximes; D010936:Plant Extracts; D000644:Quaternary Ammonium Compounds; D012997:Solvents; D013454:Sulfoxides; D014867:Water; C037759:trioctylmethylammonium; D002794:Choline; C119494:glucoraphanin; C003313:sinalbin