Piezoelectric pumping in flow analysis: Application to the spectrophotometric determination of gabapentin.
Solutions propelling devices are fundamental components of a flow-based analytical manifold. In this work different manifold configurations were implemented to evaluate the performance of multiple piezoelectric micro-pumps used as solutions insertion and propulsion devices. The micro-pumps are piezo-actuated micro-diaphragm pumps with passive check valves characterised by a small compact size and low power demands, and are able to produce reproducible flow rates of up to 4 mL min(-1). The flow rate is controlled by the frequency of the piezoelectric actuator (up to 20 Hz). As an additional feature, piezoelectric micro-pumps actuation generates a pulsed flowing stream that ensures a faster sample/reagent mixing contributing to improved reaction development. The developed flow approach was assessed in the spectrophotometric determination of gabapentin in pharmaceutical preparations upon reaction with 1,2-naphthoquinone-4-sulfonate in alkaline medium. Distinct flow manifold configurations were designed for achievement of different solutions management. Linear calibrations plots for gabapentin concentrations of up to 150 mg L(-1), with relative standard deviations of less than 1.50% (n=10) and a sample throughput of about 28 determinations per hour, were obtained.
Ribeiro
Marta F T
MF
REQUIMTE, Faculdade de Farmácia da Universidade do Porto, Rua Aníbal Cunha 164, 4099-030 Porto, Portugal.
Santos
João L M
JL
Lima
José L F C
JL
D000588:Amines / Q000032:analysis*; D003509:Cyclohexanecarboxylic Acids / Q000032:analysis*; D004581:Electronics / Q000295:instrumentation* / Q000379:methods*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D000077206:Gabapentin; D004364:Pharmaceutical Preparations / Q000032:analysis; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods*; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D000588:Amines; D003509:Cyclohexanecarboxylic Acids; D004364:Pharmaceutical Preparations; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin