title: Identification and expression analysis of novel LAGE-1 alleles with single nucleotide polymorphisms in cancer patients.
abstract: OBJECTIVE
  The purpose of this study is to identify single nucleotide polymorphisms (SNPs) in the coding region alleles of the X chromosomal LAGE-1 gene, and investigate the frequency of such SNPs in both cancer patients and healthy controls, and thus determine the potential significance of these SNPs with respect to cancer vaccine therapy.
  
  
  METHODS
  In this study, different mRNAs transcribed from the LAGE-1 gene were identified by RT-PCR from healthy donors and cancer patients samples.
  
  
  RESULTS
  A new LAGE-1 allele containing three coding region SNPs (69A/G, 317C/G, and 397T/G) were identified. The allele is highly expressed as the LAGE-1a mRNA variant AY679089 in some of the cancer patients. The three SNPs altered the LAGE-1 gene sequence to that of NYESO-1 at both the nucleotide and amino acid level.
  
  
  CONCLUSIONS
  There is a high frequency of the LAGE-1 gene allele with SNPs in coding regions in cancer patients. There was a clear relationship between the variant AY679089 and gastric cancer. The SNPs may lead to accelerated progress of poorly differentiated gastric cancer. The SNPs found in these alleles may also alter the immunological characteristics of LAGE-1a and should be taken into account if this antigen is adopted as a cancer vaccine component.
authors: Department of liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, CAMS & PUMC, Beijing 100730, China.
authors: Yi
authors: Y
authors: Shao
authors: Zhen-Yuan
authors: ZY
authors: Sun
authors: Shi-Wei
authors: SW
authors: Sun
authors: Yi
authors: Y
authors: Zhao
authors: Wan Yee
authors: WY
authors: Sin
authors: Yan-Hua
authors: YH
authors: Yuan
authors: Andrew J
authors: AJ
authors: Simpson
authors: Lloyd J
authors: LJ
authors: Old
authors: Xin-Ting
authors: XT
authors: Sang
authors: Yi-Lei
authors: YL
authors: Mao
authors: Yong
authors: Y
authors: Xie
authors: Jie-Fu
authors: JF
authors: Huang
authors: Hai-Tao
authors: HT
authors: Zhao
mesh_terms: D000483:Alleles; D000951:Antigens, Neoplasm / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D013274:Stomach Neoplasms / Q000235:genetics
chemical_list: D000951:Antigens, Neoplasm; D000954:Antigens, Surface; C510162:CTAG2 protein, human