title: Chemical and genetic diversity of Ligularia latihastata and Ligularia villosa in Yunnan Province of China.
abstract: The chemical constituents of the root extracts and the nucleotide sequences of the atpB-rbcL intergenic region of Ligularia latihastata and L. villosa, collected in northwestern Yunnan Province, were studied. In the twelve collected samples of L. latihastata, two major benzofurans, 5,6-dimethoxy-2-(1-methylethenyl)-1-benzofuran (1) and euparin (2) were detected as major components. The minor compound (2R*,3S*)-5-acetyl-2,3-dihydro-6-hydroxy-2-(1-methylethenyl)-1-benzofuran-3-yl (2Z)-2-[(acetoxy)methyl]but-2-enoate (4) was found to be susceptible to artifact formation upon extraction with EtOH. The intra-specific diversity in chemical composition of the samples was small, but the diversity in the atpB-rbcL sequence was fairly large. Compounds 1 and 2 were also found in the three collected samples of L. villosa, indicating that the two species are chemically close to each other, in agreement with morphological taxonomy.
authors: Department of Chemistry, Rikkyo University, Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan. chkkuroda@grp.rikkyo.ne.jp
authors: Chiaki
authors: C
authors: Kuroda
authors: Kota
authors: K
authors: Kiuchi
authors: Atsushi
authors: A
authors: Torihata
authors: Koichiro
authors: K
authors: Takeshita
authors: Xun
authors: X
authors: Gong
authors: Yuemao
authors: Y
authors: Shen
authors: Hiroshi
authors: H
authors: Hirota
authors: Hiroyuki
authors: H
authors: Onuki
authors: Ryo
authors: R
authors: Hanai
mesh_terms: D019659:Asteraceae / Q000737:chemistry* / Q000235:genetics*; D001572:Benzofurans / Q000032:analysis*; D002087:Butyrates / Q000032:analysis*; D002681:China; D014644:Genetic Variation; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: C524283:5,6-dimethoxy-2-(1-methylethenyl)-1-benzofuran; C524285:5-acetyl-2,3-dihydro-6-hydroxy-2-(1-methylethenyl)-1-benzofuran-3-yl 2-((acetoxy)methyl)but-2-enoate; D001572:Benzofurans; D002087:Butyrates; C524284:euparin