title: [Molecular subtyping of Vibrio cholerae isolates by pulsed-field gel electrophoresis in Shenzhen from 1993].
abstract: OBJECTIVE
  To determine the genetic relationships between different Vibrio cholerae isolates in Shenzhen from 1993 to 2002.
  
  
  METHODS
  Chromosomal DNA from 60 isolates was digested in seakem gold agrose with restriction enzyme Not I and plugs were then analyzed by pulsed-field gel electrophoresis. Pulsed-field gel electrophoresis (PFGE) patterns of V. cholerae isolates were clustered using BioNumerics software.
  
  
  RESULTS
  39 distinctive PFGE patterns were identified with each pattern having 20 to 30 bands. Most PFGE patterns were divided into cluster A or cluster B.
  
  
  CONCLUSIONS
  The closely related pandemic clone clusters of V. cholerae strains did exist in Shenzhen. PFGE of V. cholerae could be used for active surveillance and tracking for cholerae.
authors: Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.
authors: Quan-xue
authors: QX
authors: Lan
authors: Qing-hua
authors: QH
authors: Hu
authors: Xiao-lu
authors: XL
authors: Shi
authors: Bing
authors: B
authors: Wang
authors: Yi-man
authors: YM
authors: Lin
authors: Shun-xiang
authors: SX
authors: Zhang
authors: Jin-quan
authors: JQ
authors: Cheng
authors: Biao
authors: B
authors: Kan
authors: Jian-guo
authors: JG
authors: Xu
mesh_terms: D002681:China / Q000453:epidemiology; D002771:Cholera / Q000453:epidemiology / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D010802:Phylogeny; D014734:Vibrio cholerae / Q000145:classification* / Q000235:genetics*