Prevalence and natural host range of Homalodisca coagulata virus-1 (HoCV-1).
Transmission electron microscopy was used to confirm the presence of picorna-like virus particles presumed to be Homalodisca coagulata virus-1 (HoCV-1) in the midgut region of adult glassy-winged sharpshooters (GWSS). In addition, we offer a reverse transcription polymerase chain reaction (RT-PCR) assay for the detection of this virus with a sensitivity of approximately 95 genome equivalents. A survey employing this assay in conjunction with GWSS samples collected throughout the United States including California, Hawaii, Florida Georgia, and the Carolinas revealed a fairly widespread pattern of distribution, although potentially restricted to temperate regions, areas with elevated host densities, or to populations of a common origin. The virus was found to naturally infect adults regardless of host plant and was not specific to a particular lifestage or sex. Examination of alternate leafhopper species further demonstrated that, although infection is not ubiquitous to all sharpshooter genera, HoCV-1 is not limited to Homalodisca vitripennis (=H. coagulata).
Hunnicutt
L E
LE
USDA ARS US Horticultural Research Laboratory, Ft. Pierce, FL 34945, USA.
Mozoruk
J
J
Hunter
W B
WB
Crosslin
J M
JM
Cave
R D
RD
Powell
C A
CA
D000818:Animals; D006430:Hemiptera / Q000821:virology*; D007304:Insect Viruses / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D046529:Microscopy, Electron, Transmission; D015995:Prevalence; D012328:RNA Viruses / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D020133:Reverse Transcriptase Polymerase Chain Reaction