A transgenic mouse model for high content, cell cycle phenotype screening in live primary cells.
High content cell-based genetic and small molecule library screens are powerful strategies in drug discovery and investigations of disease mechanisms. We report that primary cells derived from a transgenic mouse model expressing a fluorescence mitosis biosensor provide unambiguous phenotype readouts without the need for transfection or immunocytochemistry. Phenotype profiles of cell cycle disruption and of apoptosis are easily detectable at a single time point selected from time-lapse live fluorescence microscopy. Most importantly, this transgenic mouse model may be crossed with cancer mouse models to derive biosensor-expressing primary cancer cells for use in high content screening strategies targeting discovery of tumor-specific chemotherapeutic compounds.
Burney
Richard O
RO
Department of Gynecology and Obstetrics, Stanford University School of Medicine, Stanford, California 94305, USA.
Lee
Alan I
AI
Leong
Denise E
DE
Jones
Joshua T
JT
Hahn
Angela T
AT
Meyer
Tobias
T
Yao
Mylene W M
MW
D000818:Animals; D002453:Cell Cycle / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D023421:Models, Animal; D010641:Phenotype; D011247:Pregnancy; D013045:Species Specificity