title: [Prokaryotic expression, purification, refolding and biological assays of recombinant human interleukin 4 inclusion body].
abstract: A DNA fragment encoding human interleukin 4 was obtained by PCR from pORF-hIL4 plasmid. The amplified fragment was inserted into prokaryotic expression vector PQE60 and recombinant protein was expressed in E. Coli M15 by adding isopropyl-beta-D-thiogalactoside (IPTG). The hIL-4 protein was present as insoluble inclusion bodies in the bacterial extract. After denaturation of inclusion bodies with 5 mol/L guanidine hydrochloride, the supernate was diluted to get renaturized. Then dialysis and Ni chelating chromatography were used for purification. TF-1 proliferation assay of recombinant human interleukin 4 was performed, and then rhIL-4 was fit to be used for proliferation of human dendritic cells from monocyte in vitro.
authors: State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Jiong
authors: J
authors: Li
authors: Kaijun
authors: K
authors: Cui
authors: Jing
authors: J
authors: Wen
authors: Zhiwei
authors: Z
authors: Zhao
authors: Ping
authors: P
authors: Chen
authors: Ling
authors: L
authors: Tian
authors: Bing
authors: B
authors: Kan
authors: Yanjun
authors: Y
authors: Wen
authors: Hongxin
authors: H
authors: Deng
authors: Linyu
authors: L
authors: Fan
authors: Yuquan
authors: Y
authors: Wei
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000235:genetics; D017510:Protein Folding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*
chemical_list: C508598:IL4 protein, human; D011994:Recombinant Proteins; D015847:Interleukin-4