title: Detection of Aspergillus carbonarius and other black aspergilli from grapes by DNA OLISA microarray.
abstract: Black aspergilli, and particularly Aspergillus carbonarius, are responsible for ochratoxin A production in grapes. Correct identification of these species is essential for toxicological risk assessment in grape and wine. A low-complexity oligonucleotide microarray (OLISA, Apibio, F) based on DNA oligonucleotides probes, obtained from sequences of the calmodulin gene, was set up in order to detect A. carbonarius, A. japonicus/A. aculeatus and A. ibericus isolated from grape. The designed microarray distinguished all Aspergillus species and the detection limit for A. carbonarius was 3.2 pg of DNA as a template for the PCR reaction. This microarray offers a quick and parallel analysis to detect individual Aspergillus species in pure cultures and in naturally contaminated grape samples.
authors: BioMerieux-Molecular Biology Unit, Parc Polytec, 5 rue des Berges, F-38024 Grenoble cedex, France.
authors: E
authors: E
authors: Bufflier
authors: A
authors: A
authors: Susca
authors: M
authors: M
authors: Baud
authors: G
authors: G
authors: Mulè
authors: K
authors: K
authors: Brengel
authors: A
authors: A
authors: Logrieco
mesh_terms: D001230:Aspergillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000235:genetics; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D015870:Gene Expression; D009793:Ochratoxins / Q000096:biosynthesis*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity; D027843:Vitis / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D009793:Ochratoxins; C025589:ochratoxin A