title: Telomerase-immortalized non-malignant human prostate epithelial cells retain the properties of multipotent stem cells.
abstract: Understanding prostate stem cells may provide insight into the origin of prostate cancer. Primary cells have been cultured from human prostate tissue but they usually survive only 15-20 population doublings before undergoing senescence. We report here that RC-170N/h/clone 7 cells, a clonal cell line from hTERT-immortalized primary non-malignant tissue-derived human prostate epithelial cell line (RC170N/h), retain multipotent stem cell properties. The RC-170N/h/clone 7 cells expressed a human embryonic stem cell marker, Oct-4, and potential prostate epithelial stem cell markers, CD133, integrin alpha2beta1(hi) and CD44. The RC-170N/h/clone 7 cells proliferated in KGM and Dulbecco's Modified Eagle Medium with 10% fetal bovine serum and 5 microg/ml insulin (DMEM+10% FBS+Ins.) medium, and differentiated into epithelial stem cells that expressed epithelial cell markers, including CK5/14, CD44, p63 and cytokeratin 18 (CK18); as well as the mesenchymal cell markers, vimentin, desmin; the neuron and neuroendocrine cell marker, chromogranin A. Furthermore the RC170 N/h/clone 7 cells differentiated into multi tissues when transplanted into the sub-renal capsule and subcutaneously of NOD-SCID mice. The results indicate that RC170N/h/clone 7 cells retain the properties of multipotent stem cells and will be useful as a novel cell model for studying the mechanisms of human prostate stem cell differentiation and transformation.
authors: Center for Prostate Disease Research, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
authors: Hongzhen
authors: H
authors: Li
authors: Jianjun
authors: J
authors: Zhou
authors: Jun
authors: J
authors: Miki
authors: Bungo
authors: B
authors: Furusato
authors: Yongpeng
authors: Y
authors: Gu
authors: Shiv
authors: S
authors: Srivastava
authors: David G
authors: DG
authors: McLeod
authors: Jonathan C
authors: JC
authors: Vogel
authors: Johng S
authors: JS
authors: Rhim
mesh_terms: D000368:Aged; D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002461:Cell Line, Transformed; D019070:Cell Lineage / Q000187:drug effects / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D016922:Cellular Senescence / Q000187:drug effects / Q000235:genetics; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006085:Graft Survival / Q000187:drug effects / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D016513:Mice, SCID; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010641:Phenotype; D011467:Prostate / Q000166:cytology / Q000378:metabolism*; D033581:Stem Cell Transplantation / Q000379:methods; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D014183:Transplantation, Heterologous / Q000502:physiology
chemical_list: D000943:Antigens, Differentiation; D015415:Biomarkers; D003470:Culture Media; D019098:Telomerase