Reverse transfection using antibodies against a cell surface antigen in mammalian adherent cell lines.
Reverse transfection from a solid surface has the potential to deliver genes to various cells more efficiently than conventional methods. However, the effective gene delivery from a solid surface requires an optimized extracellular matrix (ECM) for the coating of glass slides, dependent on the nature of the cells. In a search for an appropriate substrate for the universal application to multiple types of cell, we focused on cell surface antigens and examined the effects of antibodies raised against them on gene transfer from an antibody-coated surface. We found that a coating of CD29-specific antibody allowed the most effective delivery of genes by reverse transfection in every type of cell that we examined. Our results suggest that reverse transfection with antibodies against CD29 might provide a universal tool for gene delivery and cell array-based analyses.
Uchimura
Eiichiro
E
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 2-41-6 Aomi, Kohtoh-ku, Tokyo 135-0064, Japan.
Yamada
Shigeru
S
Nomura
Takashi
T
Matsumoto
Kayo
K
Fujita
Satoshi
S
Miyake
Masato
M
Miyake
Jun
J
D000818:Animals; D000937:Antigen-Antibody Reactions / Q000235:genetics* / Q000276:immunology*; D000954:Antigens, Surface / Q000235:genetics* / Q000276:immunology*; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D018390:Gene Targeting / Q000379:methods*; D006801:Humans; D014162:Transfection / Q000379:methods*
D000954:Antigens, Surface