title: TARP subtypes differentially and dose-dependently control synaptic AMPA receptor gating.
abstract: A family of transmembrane AMPA receptor regulatory proteins (TARPs) profoundly affects the trafficking and gating of AMPA receptors (AMPARs). Although TARP subtypes are differentially expressed throughout the CNS, it is unclear whether this imparts functional diversity to AMPARs in distinct neuronal populations. Here, we examine the effects of each TARP subtype on the kinetics of AMPAR gating in heterologous cells and in neurons. We report a striking heterogeneity in the effects of TARP subtypes on AMPAR deactivation and desensitization, which we demonstrate controls the time course of synaptic transmission. In addition, we find that some TARP subtypes dramatically slow AMPAR activation kinetics. Synaptic AMPAR kinetics also depend on TARP expression level, suggesting a variable TARP/AMPAR stoichiometry. Analysis of quantal synaptic transmission in a TARP gamma-4 knockout (KO) mouse corroborates our expression data and demonstrates that TARP subtype-specific gating of AMPARs contributes to the kinetics of native AMPARs at central synapses.
authors: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94143, USA.
authors: Aaron D
authors: AD
authors: Milstein
authors: Wei
authors: W
authors: Zhou
authors: Siavash
authors: S
authors: Karimzadegan
authors: David S
authors: DS
authors: Bredt
authors: Roger A
authors: RA
authors: Nicoll
mesh_terms: D000818:Animals; D002460:Cell Line; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D003627:Data Interpretation, Statistical; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects; D005110:Extracellular Space / Q000502:physiology; D015640:Ion Channel Gating / Q000187:drug effects; D007536:Isomerism; D007700:Kinetics; D015233:Models, Statistical; D009474:Neurons / Q000502:physiology; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics; D051381:Rats; D018091:Receptors, AMPA / Q000819:agonists / Q000187:drug effects* / Q000502:physiology; D013569:Synapses / Q000187:drug effects* / Q000502:physiology
chemical_list: D018076:DNA, Complementary; D009687:Nuclear Proteins; D018091:Receptors, AMPA; C413185:TARP