Isolation of the acrylamide denitrifying bacteria from a wastewater treatment system manufactured with polyacrylonitrile fiber.
Acrylamide has carcinogenicity and toxicity, so its discharge to natural water and soil systems might have an adverse impact on water quality, endangering public health and welfare. The investigation attempts to isolate acrylamide denitrifying bacteria from a wastewater treatment system manufactured with polyacrylonitrile (PAN) fiber. The goal is to elucidate the effectiveness of isolated pure strain and PAN mixed strains in treating acrylamide from synthetic wastewater. The results reveal that Ralstonia eutropha TDM-3 was isolated from the wastewater treatment system manufactured with PAN fiber. The PAN mixed strains and R. eutropha TDM-3 can consume up to 1446 mg/L acrylamide to denitrify from synthetic wastewater. Complete acrylamide removal depended on the supply of sufficient electron acceptors (nitrate). Strain R. eutropha TDM-3, Azoarcus sp. pF6, Azoarcus sp. T, and Herbaspirillum sp. G8A1 are related closely, according to the phylogenetic analyses of 16S rDNA sequences.
Wang
Chun-Chin
CC
Department of Environmental Engineering, Hungkuang University, Shalu, Taichung County, Taiwan. chunchin@sunrise.hk.edu.tw
Lee
Chi-Mei
CM
D000178:Acrylamides / Q000378:metabolism*; D000180:Acrylic Resins / Q000737:chemistry* / Q000378:metabolism; D001673:Biodegradation, Environmental; D020118:Cupriavidus necator / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification
D000178:Acrylamides; D000180:Acrylic Resins; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; C010504:polyacrylonitrile; D009584:Nitrogen