title: A previously unknown dermal blood vessel phenotype in skin inflammation.
abstract: Podoplanin and lymphatic vascular endothelial hyaluronan receptor-1 (LYVE-1) are considered as lineage markers for lymphatic vessel (LV) endothelial cells (LECs). We have recently shown that IL-3 induces de novo expression of these genes in cultured blood vessel (BV) endothelial cells (BEC). To ask, if this is trans-differentiation or activation, we analyzed inflamed skin samples and cytokine-stimulated organ-cultured skin and found a subset of blood capillaries within the papillary dermis expressing low amounts of podoplanin and LYVE-1 as well as high amounts of cytokine-inducible adhesion molecules. In contrast, neighboring lymphatic capillaries express high amounts of podoplanin, LYVE-1 and low amounts of cytokine-inducible adhesion molecules. The different response patterns to inflammatory stimuli were reproducible in cell culture, when cytokine-stimulated BEC and LEC were analyzed. These findings signify that expression of "lymphatic proteins" on BEC corresponds to cell activation.
authors: Division of General Dermatology, Department of Dermatology, Medical University of Vienna, Vienna, Austria.
authors: Marion
authors: M
authors: Gröger
authors: Heide
authors: H
authors: Niederleithner
authors: Dontscho
authors: D
authors: Kerjaschki
authors: Peter
authors: P
authors: Petzelbauer
mesh_terms: D001808:Blood Vessels / Q000378:metabolism / Q000473:pathology*; D002196:Capillaries / Q000378:metabolism; D019070:Cell Lineage; D002478:Cells, Cultured; D019040:E-Selectin / Q000096:biosynthesis; D042783:Endothelial Cells / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000473:pathology*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D007377:Interleukin-3 / Q000378:metabolism; D042601:Lymphatic Vessels / Q000378:metabolism; D010641:Phenotype; D012867:Skin / Q000098:blood supply / Q000473:pathology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis; D033921:Vesicular Transport Proteins / Q000096:biosynthesis
chemical_list: D019040:E-Selectin; D007377:Interleukin-3; C119248:LYVE1 protein, human; D019010:Vascular Cell Adhesion Molecule-1; D033921:Vesicular Transport Proteins; D018799:Intercellular Adhesion Molecule-1