A novel system for water soluble protein encapsulation with high efficiency: "micelles enhanced" polyelectrolyte capsules.
Novel "micelles enhanced" polyelectrolyte (PE) capsules based on functional templates of hybrid calcium carbonate were fabricated. Evidences suggested that the structure of capsule wall was different from that of conventional PE capsules, and the wall permeability of these PE capsules changed significantly. Lysozyme, a positively charged protein in neutral solution, was studied as a model protein to be encapsulated into the "micelles enhanced" PE capsules. Confocal laser scanning microscope was used to observe the entrapping process in real time, while UV-Vis spectroscope and scanning force microscope measurements suggested the high efficiency of encapsulation. In addition, the fluorescence recovery after photobleaching technique was employed to determine the existence form of deposited molecules. Further studies showed even negatively charged water-soluble peptides or proteins can be encapsulated into these hybrid capsules by modulating the pH value in bulk solution under its isoelectronic point as well.
Li
Xiaodong
X
Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou 310027, China. lixiaodong_yb@hotmail.com
Li
Xiaohui
X
Zhang
Jianxiang
J
Zhao
Shifang
S
Shen
Jiacong
J
D002119:Calcium Carbonate; D002214:Capsules / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods*; D008823:Micelles; D008853:Microscopy; D009113:Muramidase; D010539:Permeability; D011108:Polymers / Q000737:chemistry*; D011506:Proteins; D012995:Solubility; D013057:Spectrum Analysis; D014867:Water
D002214:Capsules; D008823:Micelles; D011108:Polymers; D011506:Proteins; D014867:Water; D009113:Muramidase; D002119:Calcium Carbonate