title: Motile membrane protrusions regulate cell-cell adhesion and migration of olfactory ensheathing glia.
abstract: Olfactory ensheathing cells (OECs) are candidates for therapeutic approaches for neural regeneration due to their ability to assist axon regrowth in central nervous system lesion models. However, little is understood about the processes and mechanisms underlying migration of these cells. We report here that novel lamellipodial protrusions, termed lamellipodial waves, are integral to OEC migration. Time-lapse imaging of migrating OECs revealed that these highly dynamic waves progress along the shaft of the cells and are crucial for mediating cell-cell adhesion. Without these waves, cell-cell adhesion does not occur and migrational rates decline. The activity of waves is modulated by both glial cell line-derived neurotrophic factor and inhibitors of the JNK and SRC kinases. Furthermore, the activity of lamellipodial waves can be modulated by Mek1, independently of leading edge activity. The ability to selectively regulate cell migration via lamellipodial waves has implications for manipulating the migratory behavior of OECs during neural repair.
authors: School of Biomedical Sciences, University of Queensland, Brisbane, Queensland, Australia.
authors: Louisa C E
authors: LC
authors: Windus
authors: Christina
authors: C
authors: Claxton
authors: Chelsea L
authors: CL
authors: Allen
authors: Brian
authors: B
authors: Key
authors: James A
authors: JA
authors: St John
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology* / Q000648:ultrastructure; D002465:Cell Movement / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000494:pharmacology; D048369:MAP Kinase Kinase 1 / Q000378:metabolism; D048670:MAP Kinase Kinase 4 / Q000037:antagonists & inhibitors; D051379:Mice; D008822:Mice, Transgenic; D009457:Neuroglia / Q000502:physiology* / Q000648:ultrastructure; D009833:Olfactory Pathways / Q000166:cytology*; D011554:Pseudopodia / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D019061:src-Family Kinases / Q000037:antagonists & inhibitors
chemical_list: D004791:Enzyme Inhibitors; D051100:Glial Cell Line-Derived Neurotrophic Factor; D019061:src-Family Kinases; D048369:MAP Kinase Kinase 1; D048670:MAP Kinase Kinase 4; C482127:Map2k1 protein, mouse