title: Specific intracellular adhesion molecule-grabbing nonintegrin R1 is not involved in the murine antibody response to pneumococcal polysaccharides.
abstract: Streptococcus pneumoniae is a microorganism that frequently causes serious infections in children, the elderly, and immunocompromised patients. We studied whether the specific intracellular adhesion molecule-grabbing nonintegrin R1 (Sign-R1) receptor, involved in the uptake of capsular polysaccharides (caps-PS) by antigen-presenting cells, is necessary for the antibody response to pneumococcal caps-PS and phosphorylcholine (PC). The antibody response to caps-PS and PC was evaluated after vaccination with soluble caps-PS (Pneumovax) and after vaccination with heat-killed S. pneumoniae. The role of Sign-R1 was investigated by using Sign-R1 knockout mice and anti-Sign-R1 monoclonal antibodies. The immunoglobulin M (IgM) and IgG antibody response to PC and caps-PS (serotypes 3 and 14) was not affected by anti-Sign-R1 monoclonal antibodies. The IgM antibody response in Sign-R1 knockout mice was comparable to the antibody response in wild-type mice. The IgG antibody response to serotype 3, but not to serotype 14, tended to be lower in Sign-R1 knockout mice compared to wild-type mice. In conclusion, we found that Sign-R1 is not involved in the IgM antibody production to PC and caps-PS serotype 3 or 14 and the IgG immune response to PC and caps-PS serotype 14. There is no direct relation between capture and uptake of caps-PS serotype 14 by Sign-R1 and the initiation of the anti-caps-PS antibody production in mice.
authors: Department of Medical Diagnostic Sciences, Laboratory of Experimental Laboratory Medicine, Faculty of Medicine, Catholic University Leuven, Leuven, Belgium.
authors: Leen
authors: L
authors: Moens
authors: Axel
authors: A
authors: Jeurissen
authors: Greet
authors: G
authors: Wuyts
authors: Padraic G
authors: PG
authors: Fallon
authors: Boon
authors: B
authors: Louis
authors: Jan L
authors: JL
authors: Ceuppens
authors: Xavier
authors: X
authors: Bossuyt
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000276:immunology* / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D037181:Lectins, C-Type / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010767:Phosphorylcholine / Q000737:chemistry; D022242:Pneumococcal Vaccines / Q000276:immunology / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000276:immunology* / Q000378:metabolism; D013296:Streptococcus pneumoniae / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D037181:Lectins, C-Type; D010767:Phosphorylcholine; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin