Nucleotidase and DNase activities in Brazilian snake venoms.
Among the myriad of enzymes present in animal venoms, nucleotidases and nucleases are poorly investigated. Herein, we studied such enzymes in 28 crude venoms of animals found in Brazil. Higher levels of ATPase, 5'-nucleotidase, ADPase, phosphodiesterase and DNase activities were observed in snake venoms belonging to Bothrops, Crotalus and Lachesis genera than to Micrurus genus. The venom of Bothrops brazili snake showed the highest nucleotidase and DNase activities, whereas that of Micrurus frontalis snake the highest alkaline phosphatase activity. On the other hand, the venoms of the snake Philodryas olfersii and the spider Loxosceles gaucho were devoid of most nucleotidase and DNase activities. Species that exhibited similar nucleotidase activities by colorimetric assays showed different banding pattern by zymography, suggesting the occurrence of structural differences among them. Hydrolysis of nucleotides showed that 1 mol of ATP is cleaved in 1 mol of pyrophosphate and 1 mol of orthophosphate, whereas 1 mol of ADP is cleaved exclusively in 2 mol of orthophosphates. Pyrophosphate is barely hydrolyzed by snake venoms. Phosphodiesterase activity was better correlated with 5'-nucleotidase, ADPase and ATPase activities than with DNase activity, evidencing that phosphodiesterases are not the main agent of DNA hydrolysis in animal venoms. The omnipresence of nucleotidase and DNase activities in viperid venoms implies a role for them within the repertoire of enzymes involved in immobilization and death of preys.
Sales
Paulo Bruno Valadão
PB
Laboratory of Pathophysiology, Institute Butantan, Brazil.
Santoro
Marcelo L
ML
D015720:5'-Nucleotidase / Q000378:metabolism; D000227:Adenine Nucleotides / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001081:Apyrase / Q000378:metabolism; D001938:Brazil; D004247:DNA / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D011756:Diphosphates / Q000378:metabolism; D004546:Elapid Venoms / Q000201:enzymology*; D017815:Elapidae; D006868:Hydrolysis; D009708:Nucleotidases / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D013045:Species Specificity; D013111:Spider Venoms / Q000201:enzymology; D014757:Viper Venoms / Q000201:enzymology*; D017819:Viperidae
D000227:Adenine Nucleotides; D011756:Diphosphates; D004546:Elapid Venoms; D010710:Phosphates; D013111:Spider Venoms; D014757:Viper Venoms; C055161:loxosceles venom; D004247:DNA; D003851:Deoxyribonucleases; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D010727:Phosphoric Diester Hydrolases; D000251:Adenosine Triphosphatases; D001081:Apyrase