Cisplatin-loaded Au-Au2S nanoparticles for potential cancer therapy: cytotoxicity, in vitro carcinogenicity, and cellular uptake.
Cisplatin is one of the most effective cytotoxic agents against cancers. Its usage, however, is limited because of severe resistance and systemic toxicity. A formulation of cisplatin-loaded Au-Au(2)S nanoparticles (NPs) with near-IR (NIR) sensitivity is reported to partly overcome this limitation in this paper. NIR sensitive Au-Au(2)S NPs were successfully synthesized by the reduction of tetrachloroauric acid (HAuCl(4)) using sodium sulfide (Na(2)S), and cisplatin was loaded onto Au-Au(2)S NPs via a MUA (11-mercaptoundecanoic acid) layer. To further investigate the biological safety of cisplatin-loaded Au-Au(2)S NPs, three different cell lines were used to investigate the acute cytotoxicity and the long-term potential carcinogenicity in vitro. Cisplatin-loaded Au-Au(2)S NPs were also tested for limited hemocompatibility in vitro. Our in vitro short and long-term data provided preliminary evidence suggesting that cisplatin-loaded Au-Au(2)S NPs with NIR sensitivity are nontoxic below the maximum recommended dosage.
Ren
Lei
L
Research Center of Biomedical Engineering, Medical College, Xiamen University, Xiamen 361005, People's Republic of China. renlei@xmu.edu.cn
Huang
Xiao-Li
XL
Zhang
Bin
B
Sun
Li-Ping
LP
Zhang
Qi-Qing
QQ
Tan
Mei-Chee
MC
Chow
Gan-Moog
GM
D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology; D015197:Carcinogenicity Tests; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000008:administration & dosage* / Q000138:chemical synthesis; D006046:Gold; D006801:Humans; D007259:Infrared Rays; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D009369:Neoplasms / Q000628:therapy*
D000970:Antineoplastic Agents; D006046:Gold; D002945:Cisplatin