title: Irradiation-induced epithelial-mesenchymal transition (EMT) related to invasive potential in endometrial carcinoma cells.
abstract: OBJECTIVE
  Epithelial-mesenchymal transition (EMT) is a process whereby cells acquire molecular alterations that facilitate cell motility and invasion. In this study, we hypothesized that ionizing irradiation would cause endometrial carcinoma cells (HEC1A) to undergo an increase of motility related to EMT.
  
  
  METHODS
  We investigated the effect of ionizing irradiation on HEC1A cell migration. Furthermore, we examined whether this enhanced invasiveness was associated with epithelial-mesenchymal transition (EMT) and Twist siRNA transfections effects in ionizing irradiation-induced HEC1A cell migratory capacity.
  
  
  RESULTS
  Ionizing irradiation leads to HEC1A cell phenotypic changes with EMT: spindle-cell shape, loss of polarity, intercellular separation, and pseudopodia formation. Ionizing irradiation leads to a 2-fold increase in HEC1A cell migration. In immunofluorescence staining of HEC1A cell, the expression of Twist, an organizer of EMT, increased by ionizing irradiation. Additionally, the irradiation-induced HEC1A cell invasion was inhibited by Twist siRNA transfections.
  
  
  CONCLUSIONS
  This report suggested that the inhibitory effect of cell invasion through targeting Twist may represent a new approach for improving the therapeutic strategy.
authors: Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya 466-8550, Japan.
authors: Hirohisa
authors: H
authors: Tsukamoto
authors: Kiyosumi
authors: K
authors: Shibata
authors: Hiroaki
authors: H
authors: Kajiyama
authors: Mikio
authors: M
authors: Terauchi
authors: Akihiro
authors: A
authors: Nawa
authors: Fumitaka
authors: F
authors: Kikkawa
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000528:radiation effects*; D016889:Endometrial Neoplasms / Q000235:genetics / Q000473:pathology*; D004847:Epithelial Cells / Q000473:pathology / Q000528:radiation effects; D005260:Female; D006801:Humans; D008648:Mesoderm / Q000473:pathology / Q000528:radiation effects; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection; D051879:Twist-Related Protein 1 / Q000235:genetics
chemical_list: D009687:Nuclear Proteins; D034741:RNA, Small Interfering; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1