[Optimization of attachment conditions for rabbit mesenchymal stem cells in cytodex 3 microcarrier culture systems].
Mesenchymal stem cells are of great value in tissue engineering and genetic engineering. To study the in vitro dynamic expansion of mesenchymal stem cells by microcarrier technology, we began the research with the investigation of attachment of rabbit mesenchymal stem cells on cytodex 3 microcarriers after inoculation. The result showed a poor attachment efficiency of 16.7% +/- 1.1% under general conditions, so the attachment efficiency must be increased through the optimization of inoculation conditions. Intermittent stirring, inoculation in 50% of the final culture volume and reduction of the fetal bovine serum concentration at inoculation all led to notable increases in attachment efficiency. Ratio of rMSCs attached in aMEM cultures was 39.8% higher than that of DMEM's on the average. When all these optimal conditions were adopted, the attachment efficiency (65.5%) was significantly higher than that (26.6%) under general conditions; accordingly the final growth extents of rMSCs were 4. 50 and 2. 01, respectively. Therefore, the optimization of attachment conditions promoted the expansion of rMSCs on microcarriers.
Jiang
Dandan
D
The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Hu
Jingbo
J
Zhou
Yan
Y
Tan
Wensong
W
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D003911:Dextrans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008863:Microspheres; D011817:Rabbits
D003911:Dextrans; C052839:Cytodex