[Technique progress in the study of protein/peptide phosphorylation].
Phosphorylation is an important protein chemical modification which plays vital roles in completion and change of protein function. There are lots of technique difficulties in this field which become the challenges of the research. In recent years, phosphorylation research has made many novel achievements profit from the breakthroughs of cross-correlation techniques. In this article, we introduce the technique development of this research field from many aspects such as detection of phosphorylated proteins, enrichment of phosphorylated proteins and peptides, improvement of biological mass spectrometry-based techniques, quantification and comparison of phosphorylated proteins and peptides.
Deng
Xin-Yu
XY
State Key Laboratory of Proteomics-Beijing Proteome Research Center-Beijing Institute of Radiation Medicine, Beijing, China. dxy0026@126.com
Jiang
Ying
Y
He
Fu-Chu
FC
D015153:Blotting, Western; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D010766:Phosphorylation; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010455:Peptides; D011506:Proteins