title: Role of subchondral bone in osteoarthritis development: a comparative study of two strains of guinea pigs with and without spontaneously occurring osteoarthritis.
abstract: OBJECTIVE
  To evaluate the relationships among cartilage and subchondral bone before and after the onset of cartilage degeneration in the Hartley guinea pig model of spontaneous osteoarthritis (OA) as compared with those in Weiser-Maple guinea pigs, which do not develop OA.
  
  
  METHODS
  Mice from each strain were used at ages 2, 3, 5, and 8 months (n = 7 at each time point). The region observed was the medial tibial plateau. Cartilage degeneration was evaluated histologically. Subchondral bone structure was evaluated based on subchondral bone plate thickness and subchondral cancellous bone trabecular parameters calculated from the microfocal computed tomography 3-dimensional reconstruction image. The bone mineral density (BMD) of the subchondral cancellous bone as well as levels of urinary N-telopeptide of type I collagen (NTX) and serum osteocalcin (OC) were measured.
  
  
  RESULTS
  In Hartley guinea pigs, the number of chondrocytes in the surface layer started to decrease at 3 months. At 8 months, fibrillation expanded to the radial zone. In Weiser-Maple guinea pigs, no cartilage degeneration was noted even at 8 months. Subchondral bone plate thickness was significantly lower in Hartley guinea pigs than in Weiser-Maple guinea pigs at 2 months. The subchondral bone had a rod-like and convex structure at 2 months in Hartley guinea pigs. BMD was significantly lower in Hartley guinea pigs than in Weiser-Maple guinea pigs at 2 months. The serum OC level was significantly higher in Hartley guinea pigs than in Weiser-Maple guinea pigs at 2 months and 3 months, whereas the urinary NTX level was significantly lower in Hartley guinea pigs at 3 months.
  
  
  CONCLUSIONS
  Subchondral bone is fragile, and bone formation may be promoted in subchondral bone before the onset of cartilage degeneration in Hartley guinea pigs. Subchondral bone may be involved in the development of OA.
authors: Tottori University, Yonago, Japan. muraoka-t@pref.tottori.jp
authors: Tomoya
authors: T
authors: Muraoka
authors: Hiroshi
authors: H
authors: Hagino
authors: Toru
authors: T
authors: Okano
authors: Makoto
authors: M
authors: Enokida
authors: Ryota
authors: R
authors: Teshima
mesh_terms: D000818:Animals; D015519:Bone Density; D001842:Bone and Bones / Q000503:physiopathology*; D002358:Cartilage, Articular / Q000503:physiopathology*; D024042:Collagen Type I / Q000652:urine*; D005260:Female; D006168:Guinea Pigs; D010003:Osteoarthritis / Q000175:diagnosis* / Q000209:etiology / Q000503:physiopathology*; D015675:Osteocalcin / Q000652:urine*; D014057:Tomography, X-Ray Computed
chemical_list: D024042:Collagen Type I; D015675:Osteocalcin