title: Cellular toxicity of various inhalable metal nanoparticles on human alveolar epithelial cells.
abstract: Nanoparticles (NPs) have a greater potential to travel through an organism via inhalation than any other larger particles, and could be more toxic due to their larger surface area and specific structural/chemical properties. The aim of this study was to evaluate in vitro biological effects of various inhalable metallic NPs (TiO2, Ag, Al, Zn, Ni). Human alveolar epithelial cells (A549) were exposed to various concentrations of NPs for 24 h. The extent of morphological damage was in the order of m-TiO2 > n-TiO2 > m-silica >> n-Ni approximately = n-Zn approximately = n-Ag approximately = n-Al and was affected in a dose-dependent manner. The extent of apoptotic damage measured with two-color flow cytometry was in the order of n-Zn > n- Ni > m-silica >> n- TiO2 > m- TiO2 > n-Al > n-Ag. The extent of apoptotic damage measured with DNA fragmentation was in the order of n-Zn approximately = m-silica > n- Ni >> m- TiO2 approximately = n- TiO2 approximately = n-Al > n-Ag, indicating no significant difference in the damages by both m-TiO2 and n-TiO2. The extents of apoptotic damages were also affected in a dose-dependent manner. Uptake of no other NPs but n-TiO2 and m-TiO2 into the cells was observed after 24 h exposure. The intracellular generation of ROS was significant with n-Zn but not with the other particles. These results demonstrated that various inhalable metallic NPs (TiO2, Ag, Al, Zn, Ni) could cause cell damages directly or indirectly. More detailed studies on the influence of size, structure, and composition of the NPs are needed to better understand their toxic mechanisms.
authors: Division of Biotechnology, College of Life Sciences and Biotechnology, Seoul National University Hospital, Seoul, Korea.
authors: Seoyoung
authors: S
authors: Park
authors: Yong Kwon
authors: YK
authors: Lee
authors: Moonju
authors: M
authors: Jung
authors: Ki Heon
authors: KH
authors: Kim
authors: Namhyun
authors: N
authors: Chung
authors: Eun-Kyung
authors: EK
authors: Ahn
authors: Young
authors: Y
authors: Lim
authors: Kweon-Haeng
authors: KH
authors: Lee
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D048429:Cell Size / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D017382:Reactive Oxygen Species