Molecular cloning of pituitary glycoprotein alpha-subunit and follicle stimulating hormone and chorionic gonadotropin beta-subunits from New World squirrel monkey and owl monkey.
The goal of this study was to characterize the gonadotropins expressed in pituitary glands of the New World squirrel monkey (Saimiri sp.) and owl monkey (Aotus sp.). The various subunits were amplified from total RNA from squirrel monkey and owl monkey pituitary glands by reverse transcription-polymerase chain reaction and the deduced amino acid sequences compared to those of other species. Mature squirrel monkey and owl monkey glycoprotein hormone alpha-polypeptides (96 amino acids in length) were determined to be 80% homologous to the human sequence. The sequences of mature beta subunits of follicle stimulating hormone (FSHbeta) from squirrel monkey and owl monkey (111 amino acids in length) are 92% homologous to human FSHbeta. New World primate glycoprotein hormone alpha-polypeptides and FSHbeta subunits showed conservation of all cysteine residues and consensus N-linked glycosylation sites. Attempts to amplify the beta-subunit of luteinizing hormone from squirrel monkey and owl monkey pituitary glands were unsuccessful. Rather, the beta-subunit of chorionic gonadotropin (CG) was amplified from pituitaries of both New World primates. Squirrel monkey and owl monkey CGbeta are 143 and 144 amino acids in length and 77% homologous with human CGbeta. The greatest divergence is in the C terminus, where all four sites for O-linked glycosylation in human CGbeta, responsible for delayed metabolic clearance, are predicted to be absent in New World primate CGbetas. It is likely that CG secreted from pituitary of New World primates exhibits a relatively short half-life compared to human CG.
Scammell
Jonathan G
JG
Department of Comparative Medicine, University of South Alabama, College of Medicine, Mobile, AL 36688, USA. jscammel@jaguar1.usouthal.edu
Funkhouser
Jane D
JD
Moyer
Felricia S
FS
Gibson
Susan V
SV
Willis
Donna L
DL
D000595:Amino Acid Sequence; D000818:Animals; D016645:Aotidae / Q000235:genetics*; D006063:Chorionic Gonadotropin / Q000235:genetics*; D003001:Cloning, Molecular; D005260:Female; D005640:Follicle Stimulating Hormone / Q000235:genetics*; D015292:Glycoprotein Hormones, alpha Subunit / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D021122:Protein Subunits / Q000235:genetics; D012453:Saimiri / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D006063:Chorionic Gonadotropin; D015292:Glycoprotein Hormones, alpha Subunit; D021122:Protein Subunits; D005640:Follicle Stimulating Hormone