Development and application of procedures for the highly sensitive quantification of cyclosarin enantiomers in hemolysed swine blood samples.
The present study was initiated to develop a sensitive method for the analysis of cyclosarin (O-cyclohexyl methylphosphonofluoridate, GF) enantiomers in biological samples utilizing classical configurations of GC-MS and automated solid phase extraction. To achieve this goal, a specific procedure had to be developed to extract cyclosarin from swine blood samples thereby stabilising and minimising the racemisation/deracemisation of its enantiomers. The chiral stationary phase was GAMMA DEX (gamma cyclodextrin), on which GF and deuterated GF enantiomers were baseline-resolved. The limit of detection was 1 pg for (-)-GF with GC-EI-MS and 5 pg for (+)-GF with GC-NCI-MS. The absolute recovery of the overall procedure for sample preparation was 85%. After an intravenous infusion of a supralethal dose of GF in anaesthetised swine only (-)-GF could be quantified, (+)-GF was not detected.
Reiter
Georg
G
Bundeswehr Institute of Pharmacology and Toxicology, Neuherbergstrasse 11, 80937 Munich, Germany. georgreiter@bundeswehr.org
Koller
Marianne
M
Thiermann
Horst
H
Dorandeu
Frederic
F
Mikler
John
J
Worek
Franz
F
D000818:Animals; D002619:Chemical Warfare Agents / Q000032:analysis* / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006461:Hemolysis; D009943:Organophosphorus Compounds / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D013552:Swine
D002619:Chemical Warfare Agents; D009943:Organophosphorus Compounds; C053914:cyclohexyl methylphosphonofluoridate