title: Simultaneous determination of diethylene glycol and propylene glycol in pharmaceutical products by HPLC after precolumn derivatization with p-toluenesulfonyl isocyanate.
abstract: A simple and reliable HPLC method was developed for the simultaneous quantitative analysis of diethylene glycol (DEG) and propylene glycol (PG) in pharmaceutical products by precolumn derivatization. The derivatization reagent p-toluenesulfonyl isocyanate (TSIC, 10 microL, 20% in ACN v/v) was added to 100 microL of the sample, and then 10 muL of water was added. The resulting derivatives were separated using a C(18)analytical column and a mobile phase composed of 0.01 M KH(2)PO(4)buffer (adjusted to pH 2.5 with phosphoric acid) and ACN (47:53 v/v) at 1 mL/min and 25 degrees C. For detection, UV light at 227 nm was used. The derivatization conditions including reaction time, temperature, and concentration of TSIC were optimized. The calibration curves were linear from 0.062 to 18.6 microg/mL (r(2)= 0.9999) and from 0.071 to 21.3 microg/mL (r(2) = 0.9999) for DEG and PG, respectively. The RSD values of intra- and interday assays were all below 4% for DEG and PG. The proposed method was then successfully applied to analyze two Armillarisin A injection samples and two spiked syrup samples.
authors: Department of Pharmaceutical Analysis, School of Pharmacy, Fudan University, Shanghai, PR China.
authors: Tao
authors: T
authors: Zhou
authors: Haiying
authors: H
authors: Zhang
authors: Gengli
authors: G
authors: Duan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005026:Ethylene Glycols / Q000032:analysis*; D017953:Isocyanates / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D019946:Propylene Glycol / Q000032:analysis*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014105:Tosyl Compounds / Q000737:chemistry*
chemical_list: D005026:Ethylene Glycols; D017953:Isocyanates; D004364:Pharmaceutical Preparations; D014105:Tosyl Compounds; C013484:diethylene glycol; D019946:Propylene Glycol