title: In vitro inhibition of oral streptococci binding to the acquired pellicle by algal lectins.
abstract: OBJECTIVE
  The initial colonization of the tooth by streptococci involves their attachment to adsorbed components of the acquired pellicle. Avoiding this adhesion may be successful in preventing caries at early stages. Salivary mucins are glycoproteins that when absorbed onto hydroxyapatite may provide binding sites for certain bacteria. Algal lectins may be especially interesting for oral antiadhesion trials because of their great stability and high specificity for mucins. This work aimed to evaluate the potential of two algal lectins to inhibit the adherence of five streptococci species to the acquired pellicle in vitro.
  
  
  RESULTS
  The lectins used were extracted from Bryothamnion triquetrum (BTL) and Bryothamnion seaforthii (BSL). Fluorescence microscopy was applied to visualize the ability of fluorescein isothiocyanate-labelled lectins to attach to the pellicle and revealed a similar capability for both lectins. Streptococcal adherence assays were performed using saliva-coated microtitre plates. BSL inhibited more than 75% of Streptococcus sanguis, Streptococcus mitis, Streptococcus sobrinus and Streptococcus mutans adherence, achieving 92% to the latter. BTL only obtained statistically significant results on S. mitis and S. sobrinus, whose adherence was decreased by 32.5% and 54.4%, respectively.
  
  
  CONCLUSIONS
  Algal lectins are able to inhibit streptococcal adherence.
  
  
  CONCLUSIONS
  Our results support the proposed application of lectins in antiadhesion therapeutics.
authors: Faculdade de Medicina de Sobral, Universidade Federal do Ceará, Fortaleza-CE, Brazil.
authors: E H
authors: EH
authors: Teixeira
authors: M H
authors: MH
authors: Napimoga
authors: V A
authors: VA
authors: Carneiro
authors: T M
authors: TM
authors: de Oliveira
authors: K S
authors: KS
authors: Nascimento
authors: C S
authors: CS
authors: Nagano
authors: J B
authors: JB
authors: Souza
authors: A
authors: A
authors: Havt
authors: V P T
authors: VP
authors: Pinto
authors: R B
authors: RB
authors: Gonçalves
authors: W R L
authors: WR
authors: Farias
authors: S
authors: S
authors: Saker-Sampaio
authors: A H
authors: AH
authors: Sampaio
authors: B S
authors: BS
authors: Cavada
mesh_terms: D000327:Adsorption; D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000254:growth & development; D044622:Dental Pellicle / Q000382:microbiology*; D017886:Durapatite / Q000378:metabolism; D056890:Eukaryota / Q000737:chemistry; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D012463:Saliva / Q000378:metabolism; D013291:Streptococcus / Q000145:classification / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology
chemical_list: D037102:Lectins; D017886:Durapatite