Effect of lecithin with vitamin-B complex and tocopheryl acetate on long-term effect of ethanol induced immunomodulatory activities.
The alcoholic liver disease usually causes overall immunological alterations which might be attributed to hepatic disease, to ethanol action, and/or to malnourishment. In the present study, efficacy of lecithin with vitamin-B complex to treat ethanol induced immunomodulatory activity was compared with the effect of lecithin alone and tocopheryl acetate (vitamin E). Ethanol (1.6 g/kg body wt/day for 12 weeks) exposure increased thiobarbituric acid reactive substance (TBARS) level, while decreased superoxide dismutase (SOD) activity and reduced glutathione (GSH) content in whole blood hemolysate of 8-10 week-old male BALB/c mice (weighing 20-30 g). The activities of transaminase (AST and ALT) enzymes, interleukin (IL)-10 and gamma interferon (IFN-gamma) elevated, while IL-2 and IL-4 reduced in mice serum due to ethanol exposure. These suggested that oxidative stress and immunomodulatory activities were interdependent and associated with ethanol induced liver damage. Lecithin treatment significantly reduced AST (32.44%), ALT (32.09%), IL-10 (25.63%) activities and TBARS content (12.76%) compared to ethanol treated group. However, lecithin with vitamin-B complex treatment, significantly reduced AST (62.83%); ALT (61.96%); IL-10 (35.88%); IFN-gamma (22.55%) activities and TBARS content (31.58%), while significantly elevated GSH content (36.49%) and SOD activity (61.21%). Tocopheryl acetate treatment significantly reduced AST (62.83%); ALT (61.54%); IL-10 (36.35%): IFN-gamma (23.28%) activities and TBARS content (35.84%). while significantly elevated GSH content (28.76%) and SOD activity (62.42%) compared to ethanol treated group. These findings persuasively argued that lecithin with vitamin-B complex was a new promising therapeutic approach in controlling ethanol induced immunomodulatory activities involving liver damage processes. Prevention of oxidative stress with correction of nutritional deficiency caused alteration in the ethanol-induced immunomodulatory activities and associated liver diseases.
Das
Subir Kumar
SK
Department of Biochemistry, Amrita Institute of Medical Sciences, Elamakkara P.O.. Cochin 682 026, India. subirkumardas@aims.amrita.edu
Gupta
Geetanjali
G
Rao
D N
DN
Vasudevan
D M
DM
D000410:Alanine Transaminase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000378:metabolism; D000431:Ethanol / Q000037:antagonists & inhibitors* / Q000633:toxicity; D005978:Glutathione / Q000378:metabolism; D006519:Hepatitis, Alcoholic / Q000188:drug therapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects; D010713:Phosphatidylcholines / Q000494:pharmacology* / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects* / Q000276:immunology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D024505:Tocopherols; D014803:Vitamin B Complex / Q000494:pharmacology* / Q000627:therapeutic use; D024502:alpha-Tocopherol / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use
D016207:Cytokines; D010713:Phosphatidylcholines; D017392:Thiobarbituric Acid Reactive Substances; D014803:Vitamin B Complex; D000431:Ethanol; D013482:Superoxide Dismutase; D000410:Alanine Transaminase; D005978:Glutathione; D024502:alpha-Tocopherol; D024505:Tocopherols