title: Development of an immunochromatographic kit for rapid diagnosis of H5 avian influenza virus infection.
abstract: Highly pathogenic avian influenza (HPAI) caused by the H5N1 subtype has given rise to serious damage in poultry industries in Asia. The virus has expanded its geographical range to Europe and Africa, posing a great risk to human health as well. For the control of avian influenza, a rapid diagnosis by detecting the causative virus and identifying its subtype is essential. In the present study, a rapid diagnosis kit combining immunochromatography with enzyme immunoassay which detects the H5 HA antigen of influenza A virus was developed using newly established anti-H5 HA monoclonal antibodies. The present kit specifically detected all of the H5 influenza viruses tested, and did not react with the other HA subtypes. H5 HA antigens were detected from swabs and tissue homogenates of chickens infected with HPAI virus strain A/chicken/Yamaguchi/7/04 (H5N1) from 2 days post inoculation. The kit showed enough sensitivity and specificity for the rapid diagnosis of HPAI.
authors: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, Japan.
authors: Yoshimi
authors: Y
authors: Tsuda
authors: Yoshihiro
authors: Y
authors: Sakoda
authors: Saori
authors: S
authors: Sakabe
authors: Tsuyoshi
authors: T
authors: Mochizuki
authors: Yasuharu
authors: Y
authors: Namba
authors: Hiroshi
authors: H
authors: Kida
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D002645:Chickens; D002845:Chromatography; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000032:analysis* / Q000276:immunology; D007124:Immunoenzyme Techniques / Q000379:methods*; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000175:diagnosis* / Q000821:virology; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011933:Reagent Kits, Diagnostic