Chemical and genetic diversity of Ligularia latihastata and Ligularia villosa in Yunnan Province of China.
The chemical constituents of the root extracts and the nucleotide sequences of the atpB-rbcL intergenic region of Ligularia latihastata and L. villosa, collected in northwestern Yunnan Province, were studied. In the twelve collected samples of L. latihastata, two major benzofurans, 5,6-dimethoxy-2-(1-methylethenyl)-1-benzofuran (1) and euparin (2) were detected as major components. The minor compound (2R*,3S*)-5-acetyl-2,3-dihydro-6-hydroxy-2-(1-methylethenyl)-1-benzofuran-3-yl (2Z)-2-[(acetoxy)methyl]but-2-enoate (4) was found to be susceptible to artifact formation upon extraction with EtOH. The intra-specific diversity in chemical composition of the samples was small, but the diversity in the atpB-rbcL sequence was fairly large. Compounds 1 and 2 were also found in the three collected samples of L. villosa, indicating that the two species are chemically close to each other, in agreement with morphological taxonomy.
Kuroda
Chiaki
C
Department of Chemistry, Rikkyo University, Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan. chkkuroda@grp.rikkyo.ne.jp
Kiuchi
Kota
K
Torihata
Atsushi
A
Takeshita
Koichiro
K
Gong
Xun
X
Shen
Yuemao
Y
Hirota
Hiroshi
H
Onuki
Hiroyuki
H
Hanai
Ryo
R
D019659:Asteraceae / Q000737:chemistry* / Q000235:genetics*; D001572:Benzofurans / Q000032:analysis*; D002087:Butyrates / Q000032:analysis*; D002681:China; D014644:Genetic Variation; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
C524283:5,6-dimethoxy-2-(1-methylethenyl)-1-benzofuran; C524285:5-acetyl-2,3-dihydro-6-hydroxy-2-(1-methylethenyl)-1-benzofuran-3-yl 2-((acetoxy)methyl)but-2-enoate; D001572:Benzofurans; D002087:Butyrates; C524284:euparin