TLC-based detection of methylated cytosine: application to aging epigenetics.
5-Methylcytosine (m(5)C) has a plethora of functions and roles in various biological processes including human diseases and aging. A TLC-based fast and simple method for quantitative determination of total genomic levels of m(5)C in DNA is described, which can be applicable to aging research with respect to rapid and high throughput screening and comparison. Using this method, an example of the analysis of global alternations of m(5)C in serially passaged human skin fibroblasts is provided, which shows age-related global hypomethylation during cellular aging in vitro. This method can be useful for screening potential modulators of aging at the level of epigenetic alterations.
Barciszewska
Mirosława Z
MZ
Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Noskowskiego 12, Poznan 61-704, Poland. miroslawa.barciszewska@ibch.poznan.pl
Barciszewska
Anna Maria
AM
Rattan
Suresh I S
SI
D044503:5-Methylcytosine / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000375:Aging / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D016922:Cellular Senescence / Q000235:genetics; D002855:Chromatography, Thin Layer / Q000379:methods*; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D010761:Phosphorus Radioisotopes; D015203:Reproducibility of Results
D010761:Phosphorus Radioisotopes; D044503:5-Methylcytosine; D000255:Adenosine Triphosphate