Comparative proteomic analysis of human adenomyosis using two-dimensional gel electrophoresis and mass spectrometry.
OBJECTIVE
To identify the differently expressed proteins in adenomyotic tissue compared with normal uterine muscle.


METHODS
Retrospective, case-controlled study.


METHODS
University-based Department of Obstetrics and Gynecology and Proteomic Research Center.


METHODS
Ten patients who underwent transabdominal hysterectomy were included in this study. Five of them were histologically diagnosed with adenomyosis and the other five age-matched patients had cervical carcinoma in situ.


METHODS
Postoperative adenomyotic tissue and normal uterine muscle samples were collected.


METHODS
The protein extracts from paired sample tissue were separated using two-dimensional gel electrophoresis. The differently expressed protein spots were detected by gel comparison using image analysis software and identified using matrix-assisted laser desorption/ionization time of flight mass spectrometry.


RESULTS
Compared with protein profiling of normal uterine muscle, there were 12 protein spots dysregulated in adenomyotic tissue. Among them, 10 protein spots were successfully identified by mass spectrometry.


CONCLUSIONS
Comparative proteomic analysis is a useful approach for the discovery of protein biomarkers for adenomyosis and understanding the pathophysiology of the disease.
Liu
Haiyuan
H
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Beijing, China.
Lang
Jinghe
J
Wang
Xiaorong
X
Wu
Shuzhen
S
D000328:Adult; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004715:Endometriosis / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008597:Menstrual Cycle; D008875:Middle Aged; D010298:Parity; D010446:Peptide Fragments / Q000302:isolation & purification; D011247:Pregnancy; D011506:Proteins / Q000235:genetics* / Q000302:isolation & purification; D020543:Proteome; D014357:Trypsin
D010446:Peptide Fragments; D011506:Proteins; D020543:Proteome; D014357:Trypsin