Effect of TRFK-5 on airway responsiveness in ovalbumin-treated guinea pigs exposed to tobacco smoke.
Tobacco smoke (TS) exposure can induce airway hyperresponsiveness, especially in asthma. A feature of asthma is eosinophilia. We hypothesized that tobacco smoke exposure enhances eosinophil responsiveness in sensitized guinea pigs. Tobacco smoke-exposed, ovalbumin (OA)-sensitized guinea pigs were treated with TRFK-5 (1.0 mg/kg, intraperitoneal), an anti-interleukin (IL)-5 agent, or its vehicle. Guinea pigs were challenged with aerosols of OA, capsaicin, histamine, and methacholine. TRFK-5 attenuated airway responsiveness to OA but not to capsaicin, histamine, or methacholine. Bronchial alveolar lavage fluid analysis confirmed TRFK-5 attenuated airway eosinophilia in OA-treated guinea pigs. Therefore, airway responsiveness to OA is enhanced by eosinophils or IL-5 itself.
Bergren
Dale R
DR
Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, Nebraska 68178, U.S.A. dbergren@creighton.edu
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D016535:Bronchial Hyperreactivity / Q000209:etiology / Q000276:immunology / Q000503:physiopathology*; D002211:Capsaicin / Q000494:pharmacology; D002465:Cell Movement; D004195:Disease Models, Animal; D004804:Eosinophils / Q000502:physiology; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D015848:Interleukin-5 / Q000037:antagonists & inhibitors* / Q000276:immunology; D008297:Male; D016210:Methacholine Chloride / Q000494:pharmacology; D010047:Ovalbumin / Q000494:pharmacology*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
D000911:Antibodies, Monoclonal; D015848:Interleukin-5; D014028:Tobacco Smoke Pollution; D016210:Methacholine Chloride; D006632:Histamine; D010047:Ovalbumin; D002211:Capsaicin