Combinatorial dapoxyl dye library and its application to site selective probe for human serum albumin.
The combinatorial fluorescent dapoxyl dye library was prepared by both solution- and solid-phase synthesis, generating 80 unique dapoxyl derivatives. A fluorescence-based screening toward human serum albumin (HSA) found one highly sensitive HSA binder ( A41-S) with over 55-fold intensity change. Displacement assay showed the selective binding of A41-S to the site I of HSA, addressing its potential to be a highly selective and sensitive HSA probe.
Min
Jaeki
J
Department of Chemistry, New York University, New York, New York 10003, USA.
Lee
Jae Wook
JW
Ahn
Young-Hoon
YH
Chang
Young-Tae
YT
D001665:Binding Sites; D004396:Coloring Agents / Q000138:chemical synthesis*; D020650:Combinatorial Chemistry Techniques; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006801:Humans; D008956:Models, Chemical; D012709:Serum Albumin / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D004396:Coloring Agents; D005456:Fluorescent Dyes; D012709:Serum Albumin