title: Ostreae Testa prevent ovariectomy-induced bone loss in mice by osteoblast activations.
abstract: OBJECTIVE
  The anti-osteoporotic effect of heated powder of Ostreae Testa (hPOT), a powder of oyster shell-Ostrea gigas Thumb (Ostrediae) was observed in vitro and in vivo.
  
  
  METHODS
  The effects on proliferation and alkaline phosphatase activity of primary osteoblasts, bone nodule formation, pit formation of osteoclasts and osteoclastogenesis were observed in vitro, and to observe the in vivo efficacy hPOT was orally administered once a day for 28 days to bilateral ovariectomy-induced osteoporosis mice at 125, 250 and 500mg/kg (of body weight).
  
  
  RESULTS
  Although hPOT did not influence the pit formation and the number of multinucleated osteoclast-like cells, osteoclastogenesis, it enhanced the proliferation of primary osteoblasts, differentiation (ALP activity) and bone nodule formation of osteoblast in vitro. The estrogen-deficient osteoporotic changes were dramatically prevented by hPOT treatment except for osteoclasts/bone perimeter.
  
  
  CONCLUSIONS
  In conclusion, hPOT prevents OVX-induced osteoporosis through osteoblasts activation effects.
authors: College of Oriental Medicine, Daegu Haany University, Gyeongsan 712-715, Republic of Korea.
authors: Sang Yeob
authors: SY
authors: Han
authors: Jong Rok
authors: JR
authors: Lee
authors: Young Kyu
authors: YK
authors: Kwon
authors: Mi Jeong
authors: MJ
authors: Jo
authors: Sook Jahr
authors: SJ
authors: Park
authors: Sang Chan
authors: SC
authors: Kim
authors: Hyeung Sik
authors: HS
authors: Lee
authors: Sae Kwang
authors: SK
authors: Ku
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects / Q000473:pathology; D008516:Medicine, Chinese Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D010024:Osteoporosis / Q000517:prevention & control*; D049895:Ostrea; D010052:Ovariectomy / Q000009:adverse effects*; D011208:Powders
chemical_list: D011208:Powders; D000469:Alkaline Phosphatase