Long-term maintenance of a transgenic Catharanthus roseus hairy root line.
Stably transformed transgenic hairy root cultures have the potential to be a valuable production platform for a variety of secondary metabolites. This study reports that a transgenic hairy root culture of Catharanthus roseus has been stably maintained for over 4.5 years. This culture carries a transgene that expresses the green fluorescent protein under the control of the glucocorticoid-inducible promoter. Genomic PCR confirmed the presence of the GFP insert within the hairy roots, and induction with dexamethasone caused a significant (p < 0.02) increase in GFP levels.
Peebles
Christie A M
CA
Department of Bioengineering, Rice University, Houston, Texas 77005, USA.
Gibson
Susan I
SI
Shanks
Jacqueline V
JV
San
Ka-Yiu
KY
D030008:Catharanthus / Q000235:genetics* / Q000254:growth & development; D049452:Green Fluorescent Proteins / Q000235:genetics; D018517:Plant Roots / Q000235:genetics; D030821:Plants, Genetically Modified / Q000254:growth & development*; D016133:Polymerase Chain Reaction
D049452:Green Fluorescent Proteins