Genotoxic effects of tanshinones from Hyptis martiusii in V79 cell line.
The genotoxic effect of two tanshinones isolated from roots of Hyptis martiussi Benth (Labiatae) was studied using V79 (Chinese hamster lung) cells by the alkaline comet assay and micronucleus test. Tanshinones were incubated with the cells at concentrations of 1, 3, 6 and 12 microg/mL for 3 h. Tanshinones were shown to be quite strongly genotoxic against V79 cells at all tested concentrations. The data obtained provide support to the view that tanshinones has DNA damaging activity in cultured V79 cells under the conditions of the assays.
Cavalcanti
B C
BC
Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Rua Cel Nunes de Melo, 1127, Caixa Postal-3157, 60430-270 Fortaleza, Ceará, Brazil.
Moura
D J
DJ
Rosa
R M
RM
Moraes
M O
MO
Araujo
E C C
EC
Lima
M A S
MA
Silveira
E R
ER
Saffi
J
J
Henriques
J A P
JA
Pessoa
C
C
Costa-Lotufo
L V
LV
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001774:Blood Chemical Analysis; D002252:Carbon Tetrachloride Poisoning / Q000473:pathology / Q000517:prevention & control*; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D005234:Fatty Liver / Q000139:chemically induced / Q000473:pathology; D005419:Flavonoids / Q000627:therapeutic use*; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; C024070:pycnogenols; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione