Selective anchoring of TFIID to nucleosomes by trimethylation of histone H3 lysine 4.
Trimethylation of histone H3 at lysine 4 (H3K4me3) is regarded as a hallmark of active human promoters, but it remains unclear how this posttranslational modification links to transcriptional activation. Using a stable isotope labeling by amino acids in cell culture (SILAC)-based proteomic screening we show that the basal transcription factor TFIID directly binds to the H3K4me3 mark via the plant homeodomain (PHD) finger of TAF3. Selective loss of H3K4me3 reduces transcription from and TFIID binding to a subset of promoters in vivo. Equilibrium binding assays and competition experiments show that the TAF3 PHD finger is highly selective for H3K4me3. In transient assays, TAF3 can act as a transcriptional coactivator in a PHD finger-dependent manner. Interestingly, asymmetric dimethylation of H3R2 selectively inhibits TFIID binding to H3K4me3, whereas acetylation of H3K9 and H3K14 potentiates TFIID interaction. Our experiments reveal crosstalk between histone modifications and the transcription factor TFIID. This has important implications for regulation of RNA polymerase II-mediated transcription in higher eukaryotes.
Vermeulen
Michiel
M
Department of Proteomics and Signal Transduction, Max-Planck-Institute for Biochemistry, D-82152 Martinsried, Germany.
Mulder
Klaas W
KW
Denissov
Sergei
S
Pijnappel
W W M Pim
WW
van Schaik
Frederik M A
FM
Varier
Radhika A
RA
Baltissen
Marijke P A
MP
Stunnenberg
Henk G
HG
Mann
Matthias
M
Timmers
H Th Marc
HT
D000107:Acetylation; D000595:Amino Acid Sequence; D000818:Animals; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008239:Lysine / Q000378:metabolism*; D046911:Macromolecular Substances / Q000378:metabolism; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D035362:Transcription Factor TFIID / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism; D016335:Zinc Fingers
D006657:Histones; D018398:Homeodomain Proteins; C512177:ING2 protein, mouse; D046911:Macromolecular Substances; D009707:Nucleosomes; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D035362:Transcription Factor TFIID; D025521:Tumor Suppressor Proteins; D008239:Lysine