title: [Study on molecular typing by pulsed-field gel electrophoresis of food Listeria monocytogenes isolates from eight province (municipalities) of China].
abstract: OBJECTIVE
  To analyse the molecular types of Listeria (L.) monocytogenes, and to construct the standard China L. monocytogenes pulsed-field gel electrophoresis (PFGE) subtyping database, using the international standardized L. monocytogenes-PFGE protocol.
  
  
  METHODS
  118 L. monocytogenes strains isolated from 8 provinces or municipalities of China were subtyped according to L. monocytogenes-PFGE protocol.
  
  
  RESULTS
  118 strains of L. monocytogenes were divided into 39 subtypes. In the 39 subtypes, 37 strains (31.36%) were GX6A160004 pattern, showing it was the predominant Lm subtype in China.
  
  
  CONCLUSIONS
  Data from molecular typing suggested that the predominant Lm strains were distributed in different regions of China. PulseNet China-Lm database was constructed which was valuable for the molecular subtyping-based surveillance of L. monocytogenes.
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors: Ren-fa
authors: RF
authors: Zhu
authors: Zhao-long
authors: ZL
authors: Gong
authors: Chang-yun
authors: CY
authors: Ye
authors: Zhi-gang
authors: ZG
authors: Cui
authors: Dong
authors: D
authors: Jin
authors: Lu
authors: L
authors: Ran
authors: Ke
authors: K
authors: Xu
authors: Lian-xiu
authors: LX
authors: Wang
authors: Wei-wei
authors: WW
authors: Chen
mesh_terms: D002681:China; D016521:Electrophoresis, Gel, Pulsed-Field; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny