Detection of microRNAs by Northern blot analyses using LNA probes.
MicroRNAs (miRNAs) are small, endogenous, non-coding RNA species, about 21 nucleotides in length, which modulate the expression of animal and plant target genes at the post-transcriptional level. It has been shown that miRNA based gene regulation plays a pivotal role in pathways involved in growth and development. Understanding miRNA mediated processes requires new technologies enabling efficient detection of small RNA species. Here we report the optimization of a miRNA Northern blot detection method based on LNA (locked nucleic acid)-modified oligonucleotide hybridization. This technique allows sensitive and highly specific detection of mature miRNAs.
Várallyay
Eva
E
Agricultural Biotechnology Center, Szent-Györgyi Albert ut 4, Gödöllö H-2100, Hungary.
Burgyán
József
J
Havelda
Zoltán
Z
D000818:Animals; D017360:Arabidopsis / Q000235:genetics; D015152:Blotting, Northern / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification
D035683:MicroRNAs; D015345:Oligonucleotide Probes; D018749:RNA, Plant