[The drug-resistant mechanism of clinical non-fermenting bacilli producing IMP-1 metalloenzyme].
A total of 50 clinical imipenem-resistant isolates of Pseudomonas aeruginosa and Acinetobacter baumannii were subjected to the ceftazidime-2- mercaptoethanol -double-disk synergy test and to the PCR assays with primers specific for bla(IMP-1). After the process of sequencing the positive one to identify the results, PCR analysis was conducted with primers specific for class 1 integrons. For synergy test, 28 isolates gave positive results, among which were 27 Pseudomonas aeruginosa and Acinetobacter baumannii. Only one Pseudomonas aeruginosa was found to carry bla(IMP-1), and bla(Int1) at the same time. This is the first ascertainment of IMP-1 producing Pseudomonas aeruginosa isolate carrying bla(IntI1) in West China, which is of significance to the research on the clinical spread of these drug-resisitant genes.
Cheng
Xi
X
Department of Microbiology, West China School of Basic Medical Science and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Jia
Wenxiang
W
Du
Baozhong
B
Zeng
Wei
W
Kang
Mei
M
Zhang
Zairong
Z
Xie
Yi
Y
Yang
Weiqing
W
Fan
Hong
H
Tao
Chuanmin
C
Zhang
Lei
L
D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002442:Ceftazidime / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005285:Fermentation; D015378:Imipenem / Q000494:pharmacology; D008623:Mercaptoethanol / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
D000900:Anti-Bacterial Agents; D008623:Mercaptoethanol; D015378:Imipenem; D002442:Ceftazidime; C409201:beta-lactamase IMP-1; D001618:beta-Lactamases