title: The plant mannose-binding lectin NTL preserves cord blood haematopoietic stem/progenitor cells in long-term culture and enhances their ex vivo expansion.
abstract: Ex vivo expansion of haematopoietic stem and progenitor cells in cytokine combinations is effective in promoting differentiation and proliferation of multilineage progenitor cells, but often results in reduction of self-renewable stem cells. This study investigated the effect of a mannose-binding lectin, NTL, purified from Narcissus tazetta var. chinensis, on prolonged maintenance and expansion of cord blood CD34+ cells. Our results showed that the presence of NTL or Flt-3 ligand (FL) significantly preserved a population of early stem/progenitor cells in a serum- and cytokine-free culture for 35 d. The effect of NTL on the ex vivo expansion of CD34+ cells in the presence of stem cell factor, thrombopoietin (TPO) and FL was also investigated. NTL-enhanced expansion of early progenitors (CD34+, CD34+CD38-, mixed colony-forming units and CFU-GEMM) and committed progenitor cells (granulocyte CFU, erythroid burst-forming units/CFU and megakayocyte CFU) after 8 and 12 d of culture. Six weeks after transplanting 12 d-expanded cells to non-obese diabetic severe combined immunodeficient mice, increased engraftment of human CD45+ cells was observed in the bone marrow of animals that received NTL-treated cells. The dual functions of NTL on long-term preservation and expansion of early stem/multilineage progenitor cells could be developed for applications in various cell therapy strategies, such as the clinical expansion of CD34+ cells for transplantation.
authors: Li Ka Shing Institute of Health Sciences, Department of Paediatrics, The Chinese Uinversity of Hong Kong, Hong Kong, China. lipang@cuhk.edu.hk
authors: Karen
authors: K
authors: Li
authors: Vincent E C
authors: VE
authors: Ooi
authors: Carmen Ka Yee
authors: CK
authors: Chuen
authors: Audrey Carmen
authors: AC
authors: Lam
authors: Linda Shiou Mei
authors: LS
authors: Ooi
authors: Xiao Bing
authors: XB
authors: Zhang
authors: Kam Sze
authors: KS
authors: Tsang
authors: Lawrence Chi Ming
authors: LC
authors: Chiu
authors: Kathy Yuen Yee
authors: KY
authors: Chan
authors: Chi Kong
authors: CK
authors: Li
authors: Tai Fai
authors: TF
authors: Fok
authors: Patrick Man Pan
authors: PM
authors: Yuen
authors: Pak Cheung
authors: PC
authors: Ng
mesh_terms: D000818:Animals; D018952:Antigens, CD34; D001793:Blood Preservation / Q000379:methods*; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D016895:Culture Media, Serum-Free; D005312:Fetal Blood / Q000166:cytology / Q000187:drug effects*; D006085:Graft Survival; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D037601:Mannose-Binding Lectin / Q000494:pharmacology*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D018515:Plant Leaves; D037121:Plant Lectins / Q000494:pharmacology*
chemical_list: D018952:Antigens, CD34; D016895:Culture Media, Serum-Free; D037601:Mannose-Binding Lectin; D037121:Plant Lectins; C065887:narcissus lectin