Enhanced cell death by AdCMV-p53 after irradiation of HeLa cells with 12C 6+ ions.
OBJECTIVE
To investigate whether the adenovirus-mediated p53 transfer could enhance the suppression of cervix adenocarcinoma by low-dose (< or =2.0Gy) heavy-ion irradiation.


METHODS
HeLa cells were exposed to C-beam, and then infected with AdCMV-p53 or GFP. P53 expression and cell cycle were detected by flow cytometric analysis. Cell apoptosis was observed under a fluorescent microscope with DAPI staining. The survival fraction was determined by colony forming assay.


RESULTS
During the observation, the proportion of p53-positive cells in the C-beam with 80 MOI p53 groups was significantly higher than that in control, C-beam only, 80 MOI p53 only, and C-beam with 40 MOI p53 (p<0.05). The proportion of cells in G(1)- or G(2)-phase in the C-beam with p53 groups was significantly higher than that in the C-beam only groups, or p53 only groups (p<0.05). The percentage of apoptotic cells for the C-beam with p53 was significantly higher than that for the C-beam only, or p53 only (p<0.05). The survival fractions for the C-beam with p53 are significantly lower than those for the C-beam only, or p53 only (p<0.05). SF2 for C-beam with 40 or 80 MOI p53 decreased to approximately 10% and approximately 4%, respectively, compared with C-beam alone (approximately 42%).


CONCLUSIONS
Adenovirus-mediated p53 transfer could enhance the suppression of cervix adenocarcinoma cells by low-dose C-beam irradiation.
Liu
Bing
B
Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China. liub626@126.com
Zhang
Hong
H
Zhou
Guangming
G
Xie
Yi
Y
Hao
Jifang
J
Zhou
Qingming
Q
Duan
Xin
X
Qiu
Rong
R
D000230:Adenocarcinoma / Q000628:therapy*; D000256:Adenoviridae / Q000235:genetics; D017209:Apoptosis / Q000528:radiation effects*; D002244:Carbon; D002455:Cell Division / Q000528:radiation effects; D003587:Cytomegalovirus / Q000235:genetics; D005260:Female; D016195:G2 Phase / Q000528:radiation effects; D016158:Genes, p53; D015316:Genetic Therapy; D006367:HeLa Cells; D063193:Heavy Ion Radiotherapy; D006801:Humans; D011401:Promoter Regions, Genetic; D002583:Uterine Cervical Neoplasms / Q000628:therapy*
D002244:Carbon