Variation in the relative copy number of the TLR7 gene in patients with systemic lupus erythematosus and healthy control subjects.
OBJECTIVE
To determine whether there is an increase in the number of TLR7 gene copies in patients diagnosed as having systemic lupus erythematosus (SLE) and whether gene amplification influences the autoantibody profiles in SLE patients, as has recently been reported in the BXSB/Yaa mouse model of lupus.


METHODS
We used a modified real-time quantitative polymerase chain reaction protocol to calculate the relative TLR7 gene copy number according to the comparative 2(-DeltaDeltaC(t) ) method in 99 SLE patients and 91 healthy controls matched for sex and ethnicity. Autoantibody profiles were determined by standard methods.


RESULTS
The relative number of TLR7 gene copies in SLE patients and healthy controls varied; however, no significant concordance between the number of relative gene copies and the SLE phenotype was found. There was also no difference in variation by ethnic group. Comparison of the relative gene copy numbers according to the presence or absence of antinuclear antibodies (ANAs), the ANA staining patterns, and the presence or absence of anti-RNA-associated antigen antibody showed no statistically significant difference in the SLE patients.


CONCLUSIONS
We determined that although the relative gene copy number of TLR7 varied in both SLE patients and healthy controls, it was not significantly increased among our SLE patients as compared with our controls. We found no detectable trend for an association between the relative gene copy number and the autoantibody profile in SLE patients.
Kelley
James
J
The University of Alabama at Birmingham, AL 35294, USA.
Johnson
Martin R
MR
Alarcón
Graciela S
GS
Kimberly
Robert P
RP
Edberg
Jeffrey C
JC
D000974:Antibodies, Antinuclear; D016022:Case-Control Studies; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000208:ethnology / Q000235:genetics*; D008297:Male; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051199:Toll-Like Receptor 7 / Q000235:genetics*
D000974:Antibodies, Antinuclear; C495348:TLR7 protein, human; D051199:Toll-Like Receptor 7