Confirmatory real-time PCR assay for human papillomavirus (HPV) type 52 infection in anogenital specimens screened for HPV infection with the linear array HPV genotyping test.
A novel real-time PCR assay for detection of human papillomavirus type 52 (HPV-52) DNA (RT-52) was evaluated on 265 anogenital samples. RT-52 had a sensitivity of 98.4% and a specificity of 100% compared to conventional HPV-52 typing assays, including hybridization of PGMY products with an HPV-52-specific probe and PCR sequencing of HPV-52 E6.
Coutlée
François
F
Département de Microbiologie et Infectiologie, Hôpital Notre-Dame du Centre Hospitalier de l'Université de Montréal, Montréal, Québec, Canada. francois.coutlee@ssss.gouv.qc.ca
Rouleau
Danielle
D
Ghattas
Georges
G
Hankins
Catherine
C
Vézina
Sylvie
S
Coté
Pierre
P
Macleod
John
J
de Pokomandy
Alexandra
A
Money
Deborah
D
Walmsley
Sharon
S
Voyer
Hélène
H
Brassard
Paul
P
Franco
Eduardo
E
D001003:Anal Canal / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005835:Genitalia / Q000821:virology*; D005838:Genotype; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D004279:DNA, Viral