[JAK3 mRNA level in graft versus host disease patients: a preliminary study].
OBJECTIVE
To quantify peripheral blood mononuclear cell JAK3 mRNA levels after allogeneic haematopoietic stem cell (allo-HSCT) transplantation, and to explore its relationship with graft vs host disease (GVHD).


METHODS
Serial monitoring of JAK3 mRNA levels by fluorescent quantitative reverse transcriptase polymerase chain reaction (FQ-RT-PCR) technique was performed on 26 cases (83 samples) after allo-HSCT.


RESULTS
The mean JAK3/ABL expression levels was 8.71 in 14 patients without GVHD; the JAK3/ABL expression level was 31.94 in one patient with acute GVHD (aGVHD); the mean JAK3/ABL expression levels was 19.23 in 11 patients with chronic GVHD (cGVHD), and the cGVHD was diagnosed at the mean time of six months (3 - 12 months). The JAK3/ABL expression levels did not change in 4 patients one month after allo-HSCT, however, the levels increased again when GVHD were diagnosed in these 4 patients, and the JAK3/ABL expression levels decreased again when GVHD was remitted.


CONCLUSIONS
The JAK3 expression level related to the remission and relapse in patients with GVHD.
Li
Qi-hui
QH
Department of Hematology, Guangdong Provincial People's Hospital, Guangzhou 510080, China.
Du
Xin
X
Geng
Su-xia
SX
Weng
Jian-yu
JY
Guo
Rong
R
Lu
Ze-sheng
ZS
Lin
Wei
W
Huang
Zi-lun
ZL
D000293:Adolescent; D000328:Adult; D005260:Female; D015870:Gene Expression; D006086:Graft vs Host Disease / Q000235:genetics*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D053616:Janus Kinase 3 / Q000235:genetics*; D008297:Male; D036102:Peripheral Blood Stem Cell Transplantation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D055815:Young Adult
D012333:RNA, Messenger; D053616:Janus Kinase 3