title: Protein expression profiling of breast cancer cells by dissociable antibody microarray (DAMA) staining.
abstract: Dissociable antibody microarray (DAMA) staining is a technology that combines protein microarrays with traditional immunostaining techniques. It can simultaneously determine the expression and subcellular location of hundreds of proteins in cultured cells and tissue samples. We developed this technology and demonstrated its application in identifying potential biomarkers for breast cancer. We compared the expression profiles of 312 proteins among three normal breast cell lines and seven breast cancer cell lines and identified 10 differentially expressed proteins by the data analysis program DAMAPEP (DAMA protein expression profiling). Among those proteins, RAIDD, Rb p107, Rb p130, SRF, and Tyk2 were confirmed by Western blot and statistical analysis to have higher expression levels in breast cancer cells than in normal breast cells. These proteins could be potential biomarkers for the diagnosis of breast cancer.
authors.affiliation: Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
authors.forename: X Cynthia
authors.initials: XC
authors.lastname: Song
authors.forename: Guanyuan
authors.initials: G
authors.lastname: Fu
authors.forename: Xufen
authors.initials: X
authors.lastname: Yang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Jiang
authors.forename: Yingjian
authors.initials: Y
authors.lastname: Wang
authors.forename: G Wayne
authors.initials: GW
authors.lastname: Zhou
mesh_terms: D000906:Antibodies / Q000378:metabolism*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000737:chemistry*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods*; D015203:Reproducibility of Results; D012984:Software
chemical_list: D000906:Antibodies; D009363:Neoplasm Proteins