title: Determination of human papillomavirus type 16 genotype and construction of cloning vector pTZ57R encoding HPV16 E7 gene.
abstract: OBJECTIVE
  To isolate and construct a cloning vector containing the human papillomavirus (HPV)16-E7 gene as a target for application as a DNA vaccine.
  
  
  METHODS
  The study was performed in 2005 in Iran. The E7 gene, one of the most important HPV oncoproteins and a target molecule for therapeutic vaccines, was amplified by polymerase chain reaction (PCR). The PCR product was cloned into a suitable cloning vector and confirmed by colony-PCR, restriction enzyme analysis, and sequenced.
  
  
  RESULTS
  The desired plasmid was sequenced and indicated 99% homology with those mentioned in the Genbank.
  
  
  CONCLUSIONS
  The Iranian HPV16 E7 gene sequence is very similar to other sequences in the Genbank, and it can be used as a candidate gene in a therapeutic vaccine for Iranian patients with cervical cancer.
authors.affiliation: Department of Virology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Meshkat
authors.forename: Hoorieh
authors.initials: H
authors.lastname: Soleimanjahi
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Mahmoudi
authors.forename: Hessam
authors.initials: H
authors.lastname: Mirshahabi
authors.forename: Zuhair M
authors.initials: ZM
authors.lastname: Hassan
authors.forename: Saeed R
authors.initials: SR
authors.lastname: Ghaffari
authors.forename: Tayebeh
authors.initials: T
authors.lastname: Sabokbar
mesh_terms: D005260:Female; D018390:Gene Targeting; D005822:Genetic Vectors; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007492:Iran; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D053918:Papillomavirus Vaccines; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000517:prevention & control / Q000821:virology*
chemical_list: D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D053918:Papillomavirus Vaccines; C059731:oncogene protein E7, Human papillomavirus type 16