Time of incubation for antifungal susceptibility testing of Aspergillus fumigatus: can MIC values be obtained at 24 hours?
A collection of Aspergillus fumigatus isolates was used to check if MICs can be read at 24 h. At 24 h, the geometric mean MIC of itraconazole for resistant isolates was determined to be 5.11 mg/liter, but the MIC was read as 16 mg/liter at 48 h. At 24 h, MICs for 51.5% of resistant strains were determined to be <or =2 mg/liter. MICs must be obtained at 48 h.
Rodriguez-Tudela
Juan Luis
JL
Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Ctra Majadahonda Pozuelo km 2, 28220 Majadahonda, Spain. jlrtudela@isciii.es
Alcazar-Fuoli
Laura
L
Alastruey-Izquierdo
Ana
A
Monzon
Araceli
A
Mellado
Emilia
E
Cuenca-Estrella
Manuel
M
D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D015203:Reproducibility of Results; D013997:Time Factors
D000935:Antifungal Agents