title: Freeze-dried platelets promote hepatocyte proliferation in mice.
abstract: In recent years, platelets are reported to promote liver, as well as bone regeneration and dermal wound healing. Platelets are required not only for thrombocytopenia treating but also for regenerative therapy. Platelets cannot be stored beyond three days, therefore, shortage of fresh platelets sometimes occurs. To preserve platelets for a long duration without degrading growth factors, a freeze-dried technique is required. We report here that platelets can be preserved by freeze-drying, using a programmed freezing method to avoid intracellular ice crystal formation. Freeze-dried platelets kept their morphological countenance and response with the agonist of thrombin was well maintained. Freeze-dried platelets stored adenine nucleotides, PDGF, and IGF-1 the same as those of fresh platelets. Freeze dried platelets also preserved their proliferative effect on hepatocytes identical to that of fresh platelets. These results of our study suggest that freeze dried platelets will obviate the storage problem of fresh platelets.
authors.affiliation: Department of Surgery, Graduate School of Comprehensive Human Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Hoshi
authors.forename: Soichiro
authors.initials: S
authors.lastname: Murata
authors.forename: Ryota
authors.initials: R
authors.lastname: Matsuo
authors.forename: Andriy
authors.initials: A
authors.lastname: Myronovych
authors.forename: Ikuka
authors.initials: I
authors.lastname: Hashimoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ikeda
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Ohkohchi
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D049109:Cell Proliferation; D018920:Coculture Techniques; D005340:Fibrinogen / Q000378:metabolism; D005612:Freeze Drying; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D010982:Platelet-Derived Growth Factor / Q000378:metabolism
chemical_list: D010982:Platelet-Derived Growth Factor; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D017228:Hepatocyte Growth Factor; D007334:Insulin-Like Growth Factor I; D000255:Adenosine Triphosphate; D005340:Fibrinogen