title: In vivo antibacterial efficacy of ultrasound after hand and rotary instrumentation in human mandibular molars.
abstract: The purpose of this prospective, randomized, single-blind study was to compare the in vivo antibacterial efficacy of a hand/rotary technique versus a hand/rotary/ultrasound technique in mesial root canals of necrotic mandibular molars. The hand/rotary group consisted of 16 mesial roots prepared with a hand/rotary technique. The hand/rotary/ultrasound group consisted of 15 mesial roots prepared similarly, followed by 1 minute of ultrasonic irrigation per canal with an ultrasonic needle in a MiniEndo unit and 15 mL/canal of 6.0% sodium hypochlorite. Canals were sampled before and after instrumentation and after 1 minute of ultrasonic irrigation. Samples were incubated anaerobically on reduced blood agar for 7 days at 37 degrees C, and colony-forming units (CFUs) were counted. The addition of 1 minute of ultrasonic irrigation resulted in significant (p = .0006) reduction in CFU count and positive cultures (p = .0047). Logistic regression analysis indicated the addition of ultrasonic irrigation was 7 times more likely to yield a negative culture.
authors.affiliation: Department of Advanced Endodontics, The Ohio State University, 305 W. 12th Avenue, Columbus, OH 43218, USA.
authors.forename: Keith
authors.initials: K
authors.lastname: Carver
authors.forename: John
authors.initials: J
authors.lastname: Nusstein
authors.forename: Al
authors.initials: A
authors.lastname: Reader
authors.forename: Mike
authors.initials: M
authors.lastname: Beck
mesh_terms: D000328:Adult; D003786:Dental Pulp Cavity / Q000000981:diagnostic imaging* / Q000382:microbiology; D004812:Epidemiologic Methods; D006801:Humans; D008334:Mandible / Q000000981:diagnostic imaging; D008963:Molar / Q000000981:diagnostic imaging*; D012388:Root Canal Irrigants / Q000627:therapeutic use; D018915:Root Canal Preparation / Q000295:instrumentation / Q000379:methods; D012399:Rotation; D013234:Stem Cells / Q000000981:diagnostic imaging; D014463:Ultrasonography
chemical_list: D012388:Root Canal Irrigants