title: [Surveillance on Rickettsia in epidemic areas of scrub typhus in Xinyang areas of Henan].
abstract: OBJECTIVE
  To understand the epidemic status of Rickettsia in Xinyang areas of Henan province.
  
  
  METHODS
  Samples including liver, spleen, kidney from mouse and chigger mites from Xinyang areas and serum samples were detected by nested-polymerase chain reaction (PCR) and indirect immunofluorescence assay (IFA).
  
  
  RESULTS
  In 62 viscus samples from mice organs, the positive rates were 16.13%, 8.06% and 6.45% for Orientia tsutsugamushi, R. typhii and Spotted fever group rickettsiae respectively. In blood clots samples from mice, the positive rates were 8.06%, 6.45% and 1.61 % for O. tsutsugamushi, R. typhii and Spotted fever group rickettsiae respectively. Three out of 26 mouse serum samples were positive for the predicted fluorexcent intensity O. tsutsugamushi.
  
  
  CONCLUSIONS
  Using nested-PCR and IFA methods, O. tsutsugamushi, R. typhii and Spotted fever group rickettsiae were detected in the captured mice living in Xinyang areas of Henan province. Results showed that there were intensive natural reserviors of Rickettsia in Henan province, suggesting that the risk of outbreak of Rickettsia in these areas was high.
authors.affiliation: Rickettsia Disease Department, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Xiu-ping
authors.initials: XP
authors.lastname: Fu
authors.forename: Jing-shan
authors.initials: JS
authors.lastname: Zhang
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Shen
authors.forename: Ming-chun
authors.initials: MC
authors.lastname: Luan
authors.forename: Meng-lei
authors.initials: ML
authors.lastname: Li
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002681:China; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007668:Kidney / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D051379:Mice; D012285:Orientia tsutsugamushi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012281:Rickettsia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D012612:Scrub Typhus / Q000453:epidemiology* / Q000382:microbiology*; D013154:Spleen / Q000382:microbiology