[Development of multifunctional envelope type artificial viral-like gene delivery system].
This review introduces a new concept "Programmed Packaging" to develop a non-viral gene delivery system. Based on this concept, multifunctional envelope type nano devices (MEND) were developed for in vitro, in situ and in vivo conditions. A quantitative study to identify a rate limiting step in intracellular trafficking was also shown between viral and non-viral vectors, which indicated an important role of controlled intranuclear disposition for development a safe and efficient non-viral gene delivery system. This review will provide a future direction of non-viral gene delivery system.
Harashima
Hideyoshi
H
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12 Nishi 6, Kita-ku, Sapporo 060-0812, Japan. harasima@pharm.hokudai.ac.jp
Kogure
Kentaro
K
Yamada
Yuma
Y
Akita
Hidetaka
H
Kamiya
Hiroyuki
H
D000818:Animals; D017209:Apoptosis; D002467:Cell Nucleus / Q000378:metabolism; D016503:Drug Delivery Systems; D004705:Endocytosis; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D008081:Liposomes; D008928:Mitochondria / Q000378:metabolism; D049329:Nanostructures; D009369:Neoplasms / Q000648:ultrastructure
D008081:Liposomes