Proteomics approach to identify dehydration responsive nuclear proteins from chickpea (Cicer arietinum L.).
Dehydration or water-deficit is one of the most important environmental stress factors that greatly influences plant growth and development and limits crop productivity. Plants respond and adapt to such stress by altering their cellular metabolism and activating various defense machineries. Mechanisms that operate signal perception, transduction, and downstream regulatory events provide valuable information about the underlying pathways involved in environmental stress responses. The nuclear proteins constitute a highly organized, complex network that plays diverse roles during cellular development and other physiological processes. To gain a better understanding of dehydration response in plants, we have developed a comparative nuclear proteome in a food legume, chickpea (Cicer arietinum L.). Three-week-old chickpea seedlings were subjected to progressive dehydration by withdrawing water and the changes in the nuclear proteome were examined using two-dimensional gel electrophoresis. Approximately 205 protein spots were found to be differentially regulated under dehydration. Mass spectrometry analysis allowed the identification of 147 differentially expressed proteins, presumably involved in a variety of functions including gene transcription and replication, molecular chaperones, cell signaling, and chromatin remodeling. The dehydration responsive nuclear proteome of chickpea revealed a coordinated response, which involves both the regulatory as well as the functional proteins. This study, for the first time, provides an insight into the complex metabolic network operating in the nucleus during dehydration.
Pandey
Aarti
A
National Institute for Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
Chakraborty
Subhra
S
Datta
Asis
A
Chakraborty
Niranjan
N
D060387:Ascorbate Peroxidases; D002467:Cell Nucleus / Q000737:chemistry; D029863:Cicer / Q000737:chemistry* / Q000201:enzymology; D016000:Cluster Analysis; D003681:Dehydration; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D015151:Immunoblotting; D013058:Mass Spectrometry; D050901:Nitrate Reductase / Q000032:analysis; D009687:Nuclear Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D010544:Peroxidases / Q000032:analysis; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D020543:Proteome / Q000737:chemistry / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D013347:Subcellular Fractions; D013482:Superoxide Dismutase / Q000032:analysis; D013997:Time Factors
D009687:Nuclear Proteins; D010940:Plant Proteins; D020543:Proteome; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D013482:Superoxide Dismutase; D050901:Nitrate Reductase