title: Bulky DNA adduct formation and risk of bladder cancer.
abstract: Exposure to polycyclic aromatic hydrocarbons (PAHs) has been associated with risk of bladder cancer and with increased bulky DNA adduct levels in several studies, mainly in smokers. We investigated the relation between bulky PAH-DNA adducts in peripheral blood mononuclear cells and bladder cancer in nonsmoking subjects from a large hospital-based case-control study in Spain. Additionally, we examined the association between DNA adduct formation and several air pollution proxies. The study comprised 76 nonsmoking cases and 76 individually matched controls by sex, region of residence, age, and smoking status (never, former). To maximize the relevance of the DNA adduct measurement as a proxy of PAH exposure, subjects selected had not changed residence, occupation, and major lifestyle factors during the last 10 years. Bulky DNA adducts were measured using the (32)P-postlabeling technique, nuclease P1 treatment. The percentage of detectable adducts was higher in controls (41%) than in cases (32%) with an odds ratio of 0.75 (95% confidence interval, 0.36-1.58). In an analysis limited to controls, a higher percentage of DNA adducts was found among those whose last residence was in a big city (50%) compared with those living in villages (19%; P = 0.04). No consistent associations were found for other markers of air pollution. In this study, among nonsmokers with stable environmental and lifestyle factors, bulky DNA adducts were not associated with bladder cancer risk. Results do not support an association of bladder cancer risk with low-level exposure to PAHs as measured through the formation of bulky DNA adducts in peripheral mononuclear cells.
authors.affiliation: Centre for Research in Environmental Epidemiology, Institut Municipal d'Investigació Mèdica, Doctor Aiguader, 88, 08003 Barcelona, Spain. gcastano@imim.es
authors.forename: Gemma
authors.initials: G
authors.lastname: Castaño-Vinyals
authors.forename: Glenn
authors.initials: G
authors.lastname: Talaska
authors.forename: Nathaniel
authors.initials: N
authors.lastname: Rothman
authors.forename: Juan
authors.initials: J
authors.lastname: Alguacil
authors.forename: Montserrat
authors.initials: M
authors.lastname: Garcia-Closas
authors.forename: Mustafa
authors.initials: M
authors.lastname: Dosemeci
authors.forename: Kenneth P
authors.initials: KP
authors.lastname: Cantor
authors.forename: Núria
authors.initials: N
authors.lastname: Malats
authors.forename: Francisco X
authors.initials: FX
authors.lastname: Real
authors.forename: Debra
authors.initials: D
authors.lastname: Silverman
authors.forename: Consol
authors.initials: C
authors.lastname: Serra
authors.forename: Alfredo
authors.initials: A
authors.lastname: Carrato
authors.forename: Adonina
authors.initials: A
authors.lastname: Tardon
authors.forename: Reina
authors.initials: R
authors.lastname: Garcia-Closas
authors.forename: Manolis
authors.initials: M
authors.lastname: Kogevinas
authors.forename: Roel
authors.initials: R
authors.lastname: Vermeulen
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000397:Air Pollution / Q000009:adverse effects*; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D018736:DNA Adducts / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011084:Polycyclic Aromatic Hydrocarbons; D012307:Risk Factors; D012424:Rural Population; D012907:Smoking / Q000009:adverse effects; D016540:Smoking Cessation; D013030:Spain; D014505:Urban Population; D001749:Urinary Bladder Neoplasms / Q000453:epidemiology / Q000235:genetics*
chemical_list: D018736:DNA Adducts; D011084:Polycyclic Aromatic Hydrocarbons; C095911:polycyclic aromatic hydrocarbons-DNA adduct