title: Simultaneous determination of Angiotensin I-converting enzyme inhibitory peptides in tryptic casein hydrolysate by high-performance liquid chromatography combined with a replicate heart-cut column-switching technique.
abstract: A replicate heart-cut column-switching HPLC method combined with two switching valves was newly developed for the simultaneous determination of three antihypertensive peptides (Ala-Phe, Tyr-Pro, and Trp-Tyr) in tryptic casein hydrolysate in one run-in assay. After a first separation on an octadecyl silane (ODS) column, heart-cuts of each peptide were individually separated on a subsequent analytical ODS column: 26% acetonitrile for Ala-Phe and Tyr-Pro (32% for Trp-Tyr) in 0.1% trifluoroacetic acid containing 10 mM sodium 1-octanesulfonate at 0.8 mL/min. Ala-Phe, Tyr-Pro, and Trp-Tyr in casein hydrolysate were determined within 70 min to be 0.377 +/- 0.037 mg/g, 2.50 +/- 0.26 mg/g, and 0.096 +/- 0.008 mg/g, respectively.
authors.affiliation: Division of Bioscience and Bioenvironmental Sciences, Faculty of Agriculture, Graduate School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Zhu
authors.forename: Toshiro
authors.initials: T
authors.lastname: Matsui
authors.forename: Keisuke
authors.initials: K
authors.lastname: Watanabe
authors.forename: Koso
authors.initials: K
authors.lastname: Shiraishi
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Ueki
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Matsumoto
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis*; D002364:Caseins / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004151:Dipeptides / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D014357:Trypsin
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D002364:Caseins; D004151:Dipeptides; C120594:tyrosyl-proline; C029951:tryptophyltyrosine; C014309:alanylphenylalanine; C475729:casein hydrolysate; D014357:Trypsin