Silicon-based microfilters for whole blood cell separation.
This paper reports on the comparison analysis of four main types of silicon-based microfilter for isolation of white blood cells (WBCs) from red blood cells (RBCs) in a given whole blood. The microfilter designs, namely, weir, pillar, crossflow, and membrane, all impose the same cut-off size of 3.5 mum to selectively trap WBCs. Using human whole blood, the microfilters have been characterized and compared for their blood handling capacity, WBCs trapping efficiency and RBCs passing efficiency. Based on the experimental results, the crossflow microfilter is superior and can be integrated with downstream components for on-chip genomic analysis.
Ji
Hong Miao
HM
Institute of Microelectronics, Singapore, Singapore.
Samper
Victor
V
Chen
Yu
Y
Heng
Chew Kiat
CK
Lim
Tit Meng
TM
Yobas
Levent
L
D001773:Blood Cells / Q000166:cytology*; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D012825:Silicon / Q000737:chemistry*; D014462:Ultrafiltration / Q000295:instrumentation* / Q000379:methods
D012825:Silicon