title: Decline in number of elevated blood CD3(+) CD56(+) NKT cells in response to intravenous immunoglobulin treatment correlates with successful pregnancy.
abstract: OBJECTIVE
  Patients with elevated blood natural killer (NK) cells may be offered intravenous immunoglobulin (IVIG) treatment, but there is controversy about the utility of blood NK cell testing. Human CD56(+) NK cells include several subpopulations that include the putatively cytotoxic CD56(+) CD16(+) subset. In mouse models of pregnant failure, NKT cells appear to be important. However, a mouse model may only be pertinent to a subset of patients, as recurrent pregnancy failure is a heterogenous group.
  
  
  METHODS
  An ethics-approved observational study was done to observe the effect of treatment on total blood lymphoid cells, and subsets of CD56(+) blood lymphocytes including CD56(+) CD3(+) NKT cells determined by flow cytometry, and to correlate with pregnancy outcome. Fifteen fertile women with a history of successful pregnancy and thirty-one women suffering from repeated implantation failure or recurrent spontaneous abortion provided serial blood samples during one menstrual cycle or prior to and during treatment. IVIG was administered to the latter group with or without heparin/aspirin.
  
  
  RESULTS
  Eight of thirty infertile women presented with high numbers of CD56(+) CD3(+) NKT cells, which declined after treatment with IVIG. The elevated NKT cell group with or without concomitant autoimmunity achieved a significantly higher successful pregnancy rate over the course of the study, as compared to women with average numbers of NKT cells and no evidence of autoimmunity (P = 0.018). Elevated NKT levels alone was an independent predictor of success on treatment (P = 0.003).
  
  
  CONCLUSIONS
  Elevated NKT cells in recurrent pregnancy loss or implantation failure can be ameliorated with IVIG treatment, and result in successful pregnancy. Assay of NKT cell numbers may identify patients who are more likely to benefit from IVIG therapy and merits further examination in randomized phase II studies.
authors.affiliation: Department of Pediatrics, University of Western Ontario, London, ON, Canada.
authors.forename: Marianne J
authors.initials: MJ
authors.lastname: van den Heuvel
authors.forename: Crystal G
authors.initials: CG
authors.lastname: Peralta
authors.forename: Kota
authors.initials: K
authors.lastname: Hatta
authors.forename: Victor K
authors.initials: VK
authors.lastname: Han
authors.forename: David A
authors.initials: DA
authors.lastname: Clark
mesh_terms: D000026:Abortion, Habitual / Q000097:blood / Q000276:immunology / Q000628:therapy*; D000328:Adult; D017252:CD3 Complex / Q000276:immunology*; D019002:CD56 Antigen / Q000276:immunology*; D015331:Cohort Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016756:Immunoglobulins, Intravenous / Q000627:therapeutic use*; D007247:Infertility, Female / Q000097:blood / Q000276:immunology / Q000628:therapy*; D007694:Killer Cells, Natural / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000276:immunology; D008875:Middle Aged; D011247:Pregnancy; D011256:Pregnancy Outcome
chemical_list: D017252:CD3 Complex; D019002:CD56 Antigen; D016756:Immunoglobulins, Intravenous