Fatty acid composition of triacylglycerols from Wharton's jelly determined by high-performance liquid chromatography.
The improved method for HPLC determination of fatty acids was proposed. The chromatographic separation of p-bromophenacyl derivatives of fatty acids under a gradient elution was achieved at 40 degrees C with an RP-18 LiChroCART 5 column and organic mobile phase containing methanol, acetonitrile, water and TEAP buffer pH 5.6. The quantitative determination of those derivatives was performed at 254 nm. Preeclampsia, the most common pregnancy complication, did not affect triacylglycerol content in the umbilical cord Wharton's jelly in comparison to the control material. However, it changed the composition of fatty acids, bound to that lipid class. The method allows the determination of almost all fatty acids forming the investigated neutral lipid class, contained in a solid tissue sample. The use of TEAP buffer excluded precipitation and flow stoppage in the HPLC system. The method reduced time and costs and might be useful for all other lipid classes and different tissues.
Romanowicz
Lech
L
Department of Medical Biochemistry, Medical University of Białystok, Białystok, Poland. lroman@amb.edu.pl
Galewska
Zofia
Z
Gogiel
Tomasz
T
Jaworski
Stefan
S
Sobolewski
Krzysztof
K
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D012997:Solvents; D014280:Triglycerides / Q000737:chemistry*; D014470:Umbilical Cord / Q000737:chemistry*
D005227:Fatty Acids; D012997:Solvents; D014280:Triglycerides