Detection of the host immune response to Burkholderia mallei heat-shock proteins GroEL and DnaK in a glanders patient and infected mice.
We examined, by enzyme-linked immunosorbent assay and Western blot analysis, the host immune response to 2 heat-shock proteins (hsps) in a patient and mice previously infected with Burkholderia mallei. The patient was the first reported human glanders case in 50 years in the United States. The expression of the groEL and dnaK operons appeared to be dependent upon a sigma(32) RNA polymerase as suggested by conserved heat-shock promoter sequences, and the groESL operon may be negatively regulated by a controlling invert repeat of chaperone expression (CIRCE) site. In the antisera, the GroEL protein was found to be more immunoreactive than the DnaK protein in both a human patient and mice previously infected with B. mallei. Examination of the supernatant of a growing culture of B. mallei showed that more GroEL protein than DnaK protein was released from the cell. This may occur similarly within an infected host causing an elevated host immune response to the B. mallei hsps.
Amemiya
Kei
K
Bacteriology Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702, USA. kei.amemiya@det.amedd.army.mil
Meyers
Jennifer L
JL
Deshazer
David
D
Riggins
Renaldo N
RN
Halasohoris
Stephanie
S
England
Marilyn
M
Ribot
Wilson
W
Norris
Sarah L
SL
Waag
David M
DM
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D042726:Burkholderia mallei / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D018834:Chaperonin 60 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005896:Glanders / Q000276:immunology* / Q000382:microbiology; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009876:Operon; D017422:Sequence Analysis, DNA
D000907:Antibodies, Bacterial; D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins; D007074:Immunoglobulin G