2-Hydroxyisoflavanone dehydratase is a critical determinant of isoflavone productivity in hairy root cultures of Lotus japonicus.
Hairy root cultures of a model legume, Lotus japonicus, were established to characterize two heterologous cDNAs encoding enzymes involved in isoflavone biosynthesis, i.e. licorice 2-hydroxyisoflavanone synthase (IFS) and soybean 2-hydroxyisoflavanone dehydratase (HID) catalyzing sequential reactions to yield isoflavones. While the control and the IFS overexpressor did not accumulate detectable isoflavones, the HID overexpressors did accumulate daidzein and genistein, showing that HID is a critical determinant of isoflavone productivity. Production of coumestrol in all the genotypes and isoliquiritigenin/liquiritigenin in IFS + HID-overexpressing lines was also noted. These results provide insight into the regulatory mechanism that controls isoflavonoid biosynthesis.
Shimamura
Masayuki
M
Department of Applied Biological Sciences, Nihon University, Fujisawa, Kanagawa, 252-8510 Japan.
Akashi
Tomoyoshi
T
Sakurai
Nozomu
N
Suzuki
Hideyuki
H
Saito
Kazuki
K
Shibata
Daisuke
D
Ayabe
Shin-Ichi
S
Aoki
Toshio
T
D002478:Cells, Cultured; D002853:Chromatography, Liquid; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D007529:Isoflavones / Q000096:biosynthesis* / Q000737:chemistry; D000070116:Lotus / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D013058:Mass Spectrometry; D008954:Models, Biological; D015394:Molecular Structure; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007529:Isoflavones; D010940:Plant Proteins; C402170:cytochrome P-450 CYP93C2 (Glycyrrhiza echinata); D003577:Cytochrome P-450 Enzyme System