title: High frequency transformation of the industrial erythromycin-producing bacterium Saccharopolyspora erythraea.
abstract: The DNA transformation in the industrial erythromycin-producing Saccharopolyspora erythraea was investigated as standard protoplast transformation methods are ineffective. Intergeneric conjugal transfer of DNA from E. coli demonstrated transformation efficiencies from 0.05 x 10(-8) to 7.2 x 10(-8) exconjugants generated per recipient. Electroporation-mediated methodologies were also established. More than 10(5) transformants were acquired per mug DNA. The proposed protocol provides an alternative route for the introduction of DNA into industrial strains.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: YiGuang
authors.initials: Y
authors.lastname: Wang
authors.forename: Siliang
authors.initials: S
authors.lastname: Zhang
mesh_terms: D019149:Bioreactors; D003227:Conjugation, Genetic; D018274:Electroporation; D004917:Erythromycin / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics; D016930:Saccharopolyspora / Q000235:genetics* / Q000378:metabolism*; D013997:Time Factors; D014170:Transformation, Genetic
chemical_list: D004917:Erythromycin