[Culture and purification of olfactory ensheathing cells from human fetal using different attachment rates combined with the technique of using NT3 intermittently].
OBJECTIVE
To explore a simple and pragmatic method to obtain sufficient olfactory ensheathing cells from human fetal by using different attachment rates in harvested cells with the combinating of the technique of using NT3 intermittently.


METHODS
DMEM/F12 culture solution including 100 mL/L of fetal bovine serum or including NT3 was used to culture olfactory ensheathing cells intermittently every 48 h. The conditions and growth degree of OECs were observed, and P75 immunocytochemistry was used to estimate the purity of the cells.


RESULTS
Human fetal OECs were positive after P75 immunocytochemistry. They appeared to be dipolar or tripolar cells and their processes formed a network in vitro. The purity of OECs in good conditions reached about 95% on 9 d and 83% on 12 d.


CONCLUSIONS
The method of using different attachment rates combined with the technique of using NT3 intermittently can culture and purify OECs simply and effectively.
Li
Qiang
Q
Department of Orthopedics, the Second Hospital, Xi'an Jiaotong University, Xi'an, China. orthli@163.com
He
Xi-Jing
XJ
Rao
Guo-Zhou
GZ
Dong
En-Xia
EX
Fan
Pei
P
Wang
Bin
B
Zhu
Zhen-Zhong
ZZ
Zang
Quan-Jin
QJ
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D005333:Fetus / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry; D009414:Nerve Growth Factors / Q000378:metabolism*; D009832:Olfactory Nerve / Q000166:cytology* / Q000378:metabolism; D013997:Time Factors
D009414:Nerve Growth Factors