[Subtype and sequence analysis of gag and env genes among HIV-1 strains circulating in Beijing residents during 2006].
OBJECTIVE
To investigate the subtype distribution and the prevalence of sequence characteristics of HIV-1 strains in Beijing residents during 2006 and to analyze the relationship between distribution of HIV-1 subtypes and transmission routines.


METHODS
Blood samples from 32 new confirmed HIV-1 infected individuals from Beijing residents in 2006 and separated plasma specimens were collected. RNAs were extracted and the gag and env gene were amplified by RT-PCR and nest-PCR. PCR products were sequenced directly and phylogenetic analyses of gag and env gene were performed using the MEGA2 software.


RESULTS
Among 32 HIV-1 plasma samples, 22 gag and 4 env gene fragments were amplified and analyzed. Five HIV-1 subtypes or circulating recombinant forms(CRFs) of HIV-1 including Thai B (2 strains), B (9 strains), C (2 strains), CRF07_BC (5 strains), CRF01 AE (4 strains) were identified being circulated in Beijing. The gene divergences of gag gene inside the subtypes were 6.6%, 4.3%, 6.8%, 4.9% and 3.0% in subtype B, Thai B, C, CRF01_AE and CRF07_BC respectively. Subtypes B were predominant in Beijing, accounted for 40.9% among 22 samples.


CONCLUSIONS
Five HIV-1 subtypes were identified in Beijing and the surveillance of HIV-1 gene variation should be paid more attention to.
Ye
Jing-rong
JR
Beijing Center for Disease Control and Prevention, Beijing 100013, China.
Xing
Hui
H
Liu
Hai-lin
HL
Hei
Fa-xin
FX
Zhao
Yue-juan
YJ
Liu
Sheng-ya
SY
Sun
Wei-dong
WD
Zhang
Qi-yun
QY
Zhang
Qin
Q
Lu
Hong-yan
HY
He
Xiong
X
Shao
Yi-ming
YM
D002681:China; D015497:HIV-1 / Q000145:classification* / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics*; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
D054299:env Gene Products, Human Immunodeficiency Virus; D054301:gag Gene Products, Human Immunodeficiency Virus