Identification of genes differentially expressed during adventitious shoot induction in Pinus pinea cotyledons by subtractive hybridization and quantitative PCR.
As part of a study aimed at understanding the physiological and molecular mechanisms involved in adventitious shoot bud formation in pine cotyledons, we conducted a transcriptome analysis to identify early-induced genes during the first phases of adventitious caulogenesis in Pinus pinea L. cotyledons cultured in the presence of benzyladenine. A subtractive cDNA library with more than 700 clones was constructed. Of these clones, 393 were sequenced, analyzed and grouped according to their putative function. Quantitative real-time PCR analysis was performed to confirm the differential expression of 30 candidate genes. Results are contrasted with available data for other species.
Alonso
Pablo
P
Area de Fisiología Vegetal, Departamento BOS, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, E-33071, Oviedo, Spain.
Cortizo
Millán
M
Cantón
Francisco R
FR
Fernández
Belén
B
Rodríguez
Ana
A
Centeno
Maria L
ML
Cánovas
Francisco M
FM
Ordás
Ricardo J
RJ
D001593:Benzyl Compounds; D018548:Cotyledon / Q000235:genetics* / Q000254:growth & development; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D015723:Gene Library; D007701:Kinetin / Q000494:pharmacology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D028223:Pinus / Q000235:genetics* / Q000254:growth & development; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000235:genetics* / Q000254:growth & development; D011687:Purines; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D046509:Tissue Culture Techniques
D001593:Benzyl Compounds; D018076:DNA, Complementary; D010937:Plant Growth Regulators; D011687:Purines; C480551:benzylaminopurine; D007701:Kinetin