Activation of granulocytes by direct interaction with dendritic cells.
Granulocytes from human peripheral blood were co-cultured with conventional dendritic cells (cDC) or plasmacytoid DCs (pDC) to examine the effects of DCs on the activation or function of granulocytes. After co-culture of granulocytes with DCs, expression of the activation markers of granulocytes (CD63 and CD64) was up-regulated, and increased expression of CD50, the activation marker and ligand for CD209 (DC-SIGN) was also observed. The interaction of granulocytes with DCs was visualized as the cluster where DCs, especially cDCs, were surrounded by granulocytes to form a 'rosette'. After co-culture of granulocytes with cDCs, the secretion of elastase from granulocytes was enhanced significantly when examined cytohistochemically and by enzyme-linked immunosorbent assay. An increase in myeloperoxidase (another activation index of granulocytes) was also observed after co-culture with DCs. These findings suggest the functional and phenotypical activation of granulocytes by interaction with DCs. Furthermore, we examined the involvement of adhesion molecules in the granulocyte-DC interaction, and found that CD209 participates to some extent in this interaction.
Tsuda
M
M
First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, Japan.
Inaba
M
M
Sakaguchi
Y
Y
Fukui
J
J
Ueda
Y
Y
Omae
M
M
Ando
Y
Y
Mukaide
H
H
Guo
K
K
Yabuki
T
T
Nakatani
T
T
Ikehara
S
S
D015703:Antigens, CD / Q000378:metabolism; D002450:Cell Communication; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology*; D006098:Granulocytes / Q000201:enzymology / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D010196:Pancreatic Elastase / Q000378:metabolism; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism; D017452:Receptors, IgG / Q000378:metabolism; D012397:Rosette Formation; D060149:Tetraspanin 30; D015854:Up-Regulation
D015703:Antigens, CD; C554586:CD63 protein, human; D010980:Platelet Membrane Glycoproteins; D017452:Receptors, IgG; D060149:Tetraspanin 30; D010196:Pancreatic Elastase