Immunogenicity of a killed Leishmania vaccine with saponin adjuvant in dogs.
Cellular and humoral immune responses of dogs to a candidate vaccine, composed of Leishmania braziliensis promastigote protein plus saponin as adjuvant, have been investigated as a pre-requisite to understanding the mechanisms of immunogenicity against canine visceral leishmaniasis (CVL). The candidate vaccine elicited strong antigenicity related to the increases of anti-Leishmania IgG isotypes, together with higher levels of lymphocytes, particularly of circulating CD8(+) T-lymphocytes and Leishmania chagasi antigen-specific CD8(+) T-lymphocytes. As indicated by the intense cell proliferation and increased nitric oxide production during in vitro stimulation by L. chagasi soluble antigens, the candidate vaccine elicited an immune activation status potentially compatible with effective control of the etiological agent of CVL.
Giunchetti
Rodolfo Cordeiro
RC
Laboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas/NUPEB, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil. giunchetti@nupeb.ufop.br
Corrêa-Oliveira
Rodrigo
R
Martins-Filho
Olindo Assis
OA
Teixeira-Carvalho
Andréa
A
Roatt
Bruno Mendes
BM
de Oliveira Aguiar-Soares
Rodrigo Dian
RD
de Souza
Juliana Vitoriano
JV
das Dores Moreira
Nádia
N
Malaquias
Luiz Cosme Cotta
LC
Mota e Castro
Luciana Lisboa
LL
de Lana
Marta
M
Reis
Alexandre Barbosa
AB
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001402:B-Lymphocytes / Q000276:immunology; D004283:Dog Diseases / Q000517:prevention & control*; D004285:Dogs; D005260:Female; D007892:Leishmania braziliensis / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000517:prevention & control / Q000662:veterinary*; D008213:Lymphocyte Activation; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis; D016052:Protozoan Vaccines / Q000276:immunology*; D012503:Saponins / Q000008:administration & dosage*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D015164:Vaccines, Inactivated
D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D016052:Protozoan Vaccines; D012503:Saponins; D015164:Vaccines, Inactivated; D009569:Nitric Oxide