title: 2-Hydroxyisoflavanone dehydratase is a critical determinant of isoflavone productivity in hairy root cultures of Lotus japonicus.
abstract: Hairy root cultures of a model legume, Lotus japonicus, were established to characterize two heterologous cDNAs encoding enzymes involved in isoflavone biosynthesis, i.e. licorice 2-hydroxyisoflavanone synthase (IFS) and soybean 2-hydroxyisoflavanone dehydratase (HID) catalyzing sequential reactions to yield isoflavones. While the control and the IFS overexpressor did not accumulate detectable isoflavones, the HID overexpressors did accumulate daidzein and genistein, showing that HID is a critical determinant of isoflavone productivity. Production of coumestrol in all the genotypes and isoliquiritigenin/liquiritigenin in IFS + HID-overexpressing lines was also noted. These results provide insight into the regulatory mechanism that controls isoflavonoid biosynthesis.
authors.affiliation: Department of Applied Biological Sciences, Nihon University, Fujisawa, Kanagawa, 252-8510 Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shimamura
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Akashi
authors.forename: Nozomu
authors.initials: N
authors.lastname: Sakurai
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Suzuki
authors.forename: Kazuki
authors.initials: K
authors.lastname: Saito
authors.forename: Daisuke
authors.initials: D
authors.lastname: Shibata
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Ayabe
authors.forename: Toshio
authors.initials: T
authors.lastname: Aoki
mesh_terms: D002478:Cells, Cultured; D002853:Chromatography, Liquid; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D007529:Isoflavones / Q000096:biosynthesis* / Q000737:chemistry; D000070116:Lotus / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D013058:Mass Spectrometry; D008954:Models, Biological; D015394:Molecular Structure; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007529:Isoflavones; D010940:Plant Proteins; C402170:cytochrome P-450 CYP93C2 (Glycyrrhiza echinata); D003577:Cytochrome P-450 Enzyme System