title: Using giant unilamellar lipid vesicle micro-patterns as ultrasmall reaction containers to observe reversible ATP synthesis/hydrolysis of F0F1-ATPase directly.
abstract: F(0)F(1)-ATPase within chromatophores, which was labeled with pH-sensitive quantum dots, was encapsulated in large unilamellar lipid vesicles (LUVs) through reverse-phase evaporation. Then a microarray of chromatophore-containing LUVs was created using a micro-contact printing (mu-CP) technique. Through controlled dehydration-rehydration of the lipid patterns, a microarray of single chromatophore-containing giant unilamellar lipid vesicles (GUVs) was formed with desired size and uniform shape. The reversible ATP synthesis/hydrolysis of F(0)F(1)-ATPase in GUVs was directly observed by fluorescence microscopy through the fluorescence intensity increase/decrease in the pH-sensitive quantum dots labeled on the outer surface of the chromatophore. To the best of our knowledge, this is the first direct observation of the reversible behavior of F(0)F(1)-ATPase at the bulk scale.
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
authors.forename: Jiachang
authors.initials: J
authors.lastname: Yue
authors.forename: Peidong
authors.initials: P
authors.lastname: Jiang
authors.forename: Zhenxi
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D050356:Lipid Metabolism; D006180:Proton-Translocating ATPases / Q000378:metabolism*
chemical_list: D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases