title: ETV5 is required for continuous spermatogenesis in adult mice and may mediate blood testes barrier function and testicular immune privilege.
abstract: The transcription factor Ets-variant gene 5 (ETV5) is essential for spermatogonial stem cell (SSC) self-renewal, as the targeted deletion of the Etv5 gene in mice (Etv5(-/-)) results in only the first wave of spermatogenesis. Reciprocal transplants of neonatal germ cells from wild-type (WT) and Etv5(-/-) testes were performed to determine the role of ETV5 in Sertoli cells and germ cells. ETV5 appears to be needed in both cell types for normal spermatogenesis. In addition, Etv5(-/-) recipients displayed increased interstitial inflammation and tubular involution after transplantation. Preliminary studies suggest that the blood-testis barrier (Sertoli-Sertoli tight junctional complex) is abnormal in the Etv5(-/-) mouse.
authors.affiliation: Department of Veterinary Bioscience, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: Carla M K
authors.initials: CM
authors.lastname: Morrow
authors.forename: Chris E
authors.initials: CE
authors.lastname: Hostetler
authors.forename: Mike D
authors.initials: MD
authors.lastname: Griswold
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Hofmann
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Murphy
authors.forename: Paul S
authors.initials: PS
authors.lastname: Cooke
authors.forename: Rex A
authors.initials: RA
authors.lastname: Hess
mesh_terms: D000818:Animals; D001814:Blood-Testis Barrier / Q000276:immunology / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D007113:Immunity, Innate / Q000235:genetics*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008954:Models, Biological; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000276:immunology*; D019108:Tight Junctions / Q000276:immunology / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; C513242:Etv5 protein, mouse; D014157:Transcription Factors