Characterization of circulating DNA in healthy human plasma.
BACKGROUND
The importance of circulating DNA has been recognized since the detection of mutated oncogene products in cancer patients; however, there is little information about circulating DNA in normal human plasma. We characterized circulating DNA in normal human plasma to obtain basic information.


METHODS
Circulating DNA was purified from plasma samples obtained from 10 healthy donors and examined. Purified DNA was cloned and their sequence determined and analyzed. The terminal structure was examined by a labeling method.


RESULTS
The DNA levels in normal plasma samples were quite low (3.6-5.0 ng/ml). All 556 clones analyzed were independent, and obtained from various chromosomes and various regions of the gene. The mean values of their length and GC content were 176 bp and 53.7%, respectively. Their 5' and 3' ends were rich in C and G, respectively, and they presented as 5' protruding forms of double-stranded DNA in plasma.


CONCLUSIONS
Circulating DNA in normal human plasma is derived from apoptotic cells but not from necrotic cells. Structural characteristics of the circulating DNA might be associated with their stability in plasma.
Suzuki
Nobuyasu
N
Department of Biomolecular Science, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan.
Kamataki
Akihisa
A
Yamaki
Junko
J
Homma
Yoshimi
Y
D001483:Base Sequence; D004247:DNA / Q000097:blood*; D006801:Humans; D012016:Reference Values; D017422:Sequence Analysis, DNA
D004247:DNA