Shock waves activate in vitro cultured progenitors and precursors of cardiac cell lineages from the human heart.
Postischemic cardiomyopathy remains one of the disorders in urgent need of effective noninvasive therapy. It is currently accepted that the isolation, expansion and application of resident cardiac stem cells may hold therapeutic promise for the future. Recently, it has been demonstrated that shock waves (SW) could enhance the expression of vascular endothelial growth factor (VEGF) and its receptor, Flt-1. As the development of angiogenic noninvasive therapy is very important for future therapeutic strategies in cardiovascular diseases, we examined in vitro, the effects of SW treatment on adult resident cardiac primitive cells isolated from bioptic fragments of normal human hearts and from explanted pathologic hearts with postischemic cardiomyopathy. This study demonstrates that SW have positive influence on both the proliferation and the differentiation of cardiomyocytes, smooth muscle and endothelial cells precursors, with a more obvious effect being evident in the cells from normal heart than in those taken from pathologic hearts. Our results suggest that SW treatment could inhibit or retard the pathologic remodeling and functional degradation of the heart if applied during the early stages of heart failure.
Nurzynska
Daria
D
Department of Biomorphological and Functional Sciences, University of Naples Federico II, Naples, Italy.
Di Meglio
Franca
F
Castaldo
Clotilde
C
Arcucci
Alessandro
A
Marlinghaus
Ernst
E
Russo
Sergio
S
Corrado
Bruno
B
de Santo
Luca
L
Baldascino
Francesco
F
Cotrufo
Maurizio
M
Montagnani
Stefania
S
D000328:Adult; D015415:Biomarkers / Q000032:analysis; D002311:Cardiomyopathy, Dilated / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008096:Lithotripsy; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000737:chemistry / Q000166:cytology* / Q000473:pathology; D013234:Stem Cells / Q000502:physiology*
D015415:Biomarkers