[Correlation study between cadaverine level in saliva and halitosis].
OBJECTIVE
To develop a high-performance liquid chromato-graphic (HPLC) method for the determination of cadaverine in saliva and investigate the relationship between cadaverine level and organoleptic test results of halitosis.


METHODS
Saliva samples from 56 subjects were derived. 1,6-hexanediamine was used as internal standard solution. The mobile phase of the HPLC was composed of solution A, methanol; solution B, V(water):V(phosphoric acid):V(triethylamine)=360:9:6 at a flow rate of 1 ml/minute. Detection was carried out at a wavelength of 231 nm. Organoleptic test scores were recorded by 2 judges, according to a 0 to 5 scale corresponding to the degree of halitosis. The relativity between cadaverine levels in saliva and organoleptic test results was analyzed by Pearson correlation method using SPSS11.0 software package.


RESULTS
The standard curve was linear over the concentration range of 5-80 nmol/ml for cadaverine with correlation coefficient 0.9985. The recovery was 91.5% with RSD 6.93%. Cadaverine levels in saliva correlated with organoleptic test results(P<0.01).


CONCLUSIONS
The correlation between cadaverine levels in saliva and halitosis suggests that the determination of cadaverine levels by HPLC method can be used in clinical diagnosis of halitosis.
Ye
Wei
W
Department of Preventive and Pediatric Dentistry, College of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
Li
Yun-wu
YW
Feng
Xi-ping
XP
D002103:Cadaverine / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006209:Halitosis / Q000175:diagnosis*; D006801:Humans; D012463:Saliva / Q000737:chemistry*
D002103:Cadaverine