Identification of a mutation in the SV40 capsid protein VP1 that influences plaque morphology, vacuolization, and receptor usage.
A plaque variant of SV40 that was first isolated in the 1960s, designated SV40-LP(KT), was molecularly cloned and subjected to sequence analysis. The genome of SV40-LP(KT) was found to be nearly identical to the previously described isolate known as 777. However, SV40-LP(KT) contained a mutation in the VP1 coding region resulting in a change of histidine 136 to tyrosine. This VP1 mutation was identified as a genetic determinant influencing a number of phenotypes associated with SV40-LP(KT) such as plaque morphology, intracellular vacuole formation, and ganglioside receptor usage.
Murata
Haruhiko
H
Cancer Prevention Fellowship Program, Office of Preventive Oncology, Division of Cancer Prevention, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Peden
Keith
K
Lewis
Andrew M
AM
D019943:Amino Acid Substitution; D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005732:Gangliosides / Q000502:physiology; D005814:Genes, Viral; D046529:Microscopy, Electron, Transmission; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D010641:Phenotype; D020836:Protein Structure, Quaternary; D011991:Receptors, Virus / Q000502:physiology; D013539:Simian virus 40 / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D014617:Vacuoles / Q000821:virology; D010948:Viral Plaque Assay
D036022:Capsid Proteins; D005732:Gangliosides; D011991:Receptors, Virus; C062481:VP1 protein, polyomavirus