title: Sinusoidal endothelial cell repopulation following ischemia/reperfusion injury in rat liver transplantation.
abstract: We evaluated the kinetics by which rat liver sinusoidal endothelial cells (LSECs) are repopulated in the reperfused transplanted liver after 18 hours of cold ischemic storage. We found that the majority of LSECs in livers cold-stored for 18 hours in University of Wisconsin solution are seriously compromised and often are retracted before transplantation. Sinusoids rapidly re-endothelialize within 48 hours of transplantation, and repopulation is coincident with up-regulation of hepatocyte vascular endothelial growth factor expression and vascular endothelial growth factor receptor-2 expression on large vessel endothelial cells and repopulating LSECs. Although re-endothelialization occurs rapidly, we show here, using several high-resolution imaging techniques and 2 different rat liver transplantation models, that engraftment of bone marrow-derived cells into functioning LSECs is routinely between 1% and 5%.
  
  
  CONCLUSIONS
  Bone marrow plays a measurable but surprisingly limited role in the rapid repopulation and functional engraftment of bone marrow-derived LSECs after cold ischemia and warm reperfusion.
authors.affiliation: Cell Biology and Physiology and the Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA, USA. dstolz@pitt.edu
authors.forename: Donna Beer
authors.initials: DB
authors.lastname: Stolz
authors.forename: Mark A
authors.initials: MA
authors.lastname: Ross
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ikeda
authors.forename: Koji
authors.initials: K
authors.lastname: Tomiyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Kaizu
authors.forename: David A
authors.initials: DA
authors.lastname: Geller
authors.forename: Noriko
authors.initials: N
authors.lastname: Murase
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D042783:Endothelial Cells / Q000502:physiology* / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure*; D008115:Liver Regeneration / Q000502:physiology*; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury