A protective and broadly cross-neutralizing epitope of human papillomavirus L2.
We generated a monoclonal antibody, RG-1, that binds to highly conserved L2 residues 17 to 36 and neutralizes human papillomavirus 16 (HPV16) and HPV18. Passive immunotherapy with RG-1 was protective in mice. Antiserum to the HPV16 L2 peptide comprising residues 17 to 36 (peptide 17-36) neutralized pseudoviruses HPV5, HPV6, HPV16, HPV 18, HPV31, HPV 45, HPV 52, HPV 58, bovine papillomavirus 1, and HPV11 native virions. Depletion of HPV16 L2 peptide 17-36-reactive antibodies from cross-neutralizing rabbit and human L2-specific sera abolished cross-neutralization and drastically reduced neutralization of the cognate type. This cross-neutralization of diverse HPVs associated with cervical cancer, genital warts, and epidermodysplasia verruciformis suggests the possibility of a broadly protective, peptide-based vaccine.
Gambhira
Ratish
R
Department of Pathology, The Johns Hopkins University, Baltimore, Maryland 21231, USA.
Karanam
Balasubramanyam
B
Jagu
Subhashini
S
Roberts
Jeffrey N
JN
Buck
Christopher B
CB
Bossis
Ioannis
I
Alphs
Hannah
H
Culp
Timothy
T
Christensen
Neil D
ND
Roden
Richard B S
RB
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology*; D000914:Antibodies, Viral / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology*; D003429:Cross Reactions; D052162:Human papillomavirus 16 / Q000276:immunology / Q000472:pathogenicity; D006801:Humans; D007116:Immunization, Passive; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D027383:Papillomaviridae / Q000145:classification* / Q000276:immunology*; D030361:Papillomavirus Infections / Q000517:prevention & control
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins; C063316:L2 protein, Human papillomavirus type 16; C099170:L2 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral