title: Development and function of murine B220+CD11c+NK1.1+ cells identify them as a subset of NK cells.
abstract: Lymphoid organs contain a B220(+)CD11c(+)NK1.1(+) cell population that was recently characterized as a novel dendritic cell (DC) subset that functionally overlaps with natural killer (NK) cells and plasmacytoid DCs (PDCs). Using Siglec-H and NK1.1 markers, we unambiguously dissected B220(+)CD11c(+) cells and found that PDCs are the only professional interferon (IFN)-alpha-producing cells within this heterogeneous population. In contrast, B220(+)CD11c(+)NK1.1(+) cells are a discrete NK cell subset capable of producing higher levels of IFN-gamma than conventional NK cells. Unlike DCs, only a minute fraction of B220(+)CD11c(+)NK1.1(+) cells in the spleen expressed major histocompatibility complex class II ex vivo or after stimulation with CpG. Consistent with being a NK cell subset, B220(+)CD11c(+)NK1.1(+) cells depended primarily on interleukin 15 and common cytokine receptor gamma chain signaling for their development. In terms of function, expression of distinctive cell surface receptors, and location in lymphoid organs, NK1.1(+)B220(+)CD11c(+) appear to be the murine equivalent of human CD56(bright) NK cells.
authors.affiliation: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: Amanda L
authors.initials: AL
authors.lastname: Blasius
authors.forename: Winfried
authors.initials: W
authors.lastname: Barchet
authors.forename: Marina
authors.initials: M
authors.lastname: Cella
authors.forename: Marco
authors.initials: M
authors.lastname: Colonna
mesh_terms: D000818:Animals; D000950:Antigens, Ly; D000954:Antigens, Surface / Q000032:analysis*; D001854:Bone Marrow Cells / Q000276:immunology; D039521:CD11c Antigen / Q000032:analysis*; D003713:Dendritic Cells / Q000276:immunology; D007371:Interferon-gamma / Q000378:metabolism; D019409:Interleukin-15 / Q000502:physiology*; D007694:Killer Cells, Natural / Q000276:immunology*; D037181:Lectins, C-Type / Q000032:analysis*; D017493:Leukocyte Common Antigens / Q000032:analysis*; D008198:Lymph Nodes / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology*; D051379:Mice; D055651:NK Cell Lectin-Like Receptor Subfamily B; D018121:Receptors, Cytokine / Q000502:physiology; D013154:Spleen / Q000276:immunology
chemical_list: D000950:Antigens, Ly; D000954:Antigens, Surface; D039521:CD11c Antigen; D019409:Interleukin-15; C527203:KLRB1 protein, human; C527211:Klrb1c protein, mouse; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D018121:Receptors, Cytokine; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens