title: Sealing ability of dentin adhesives/desensitizer.
abstract: OBJECTIVE
  This in vitro study evaluated the sealing ability of dentin adhesives and a desensitizer.
  
  
  METHODS
  Standardized Class V cavities were prepared on the buccal and lingual surfaces of 55 freshly extracted human molars. The teeth were cut into two sections in a mesio-distal direction. All specimens were randomly divided into 11 groups (n = 10). In each group, the cavities were treated with one of eight different dentin adhesives, a dentin desensitizer or a special combination of these, except for the control group. After all the cavities had been stained for 24 hours with 0.5% methlyene blue solution, the teeth were longitudinally cut into two sections through the center of the cavities. Dye penetration was recorded according to the stained areas. Epoxy resin replicas of two specimens per group were analyzed by SEM.
  
  
  RESULTS
  All dentin adhesives/desensitizer significantly reduced dentinal permeability (p < 0.05). The sealing ability of the different dentin adhesives/desensitizer was significantly different (p < 0.001). The degree of dye penetration corresponded well with the surface morphology of the dentin surfaces after the various treatments.
  
  
  CONCLUSIONS
  None of the dentin adhesives/desensitizer could completely block fluid percolation through the dentinal tubules, but current dentin adhesives/desensitizers can significantly reduce dentin permeability.
authors.affiliation: Dept of Stomatology, No 1 Hospital Aff to Medical College, Zhejiang University, Hangzhou, China. baiping_fu@yahoo.com
authors.forename: Baiping
authors.initials: B
authors.lastname: Fu
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Shen
authors.forename: Huiming
authors.initials: H
authors.lastname: Wang
authors.forename: Matthias
authors.initials: M
authors.lastname: Hannig
mesh_terms: D004396:Coloring Agents; D001840:Dental Bonding; D003737:Dental Cavity Preparation / Q000145:classification; D003738:Dental Cements / Q000737:chemistry; D003763:Dental Leakage / Q000145:classification; D003764:Dental Materials / Q000737:chemistry*; D003804:Dentin / Q000648:ultrastructure; D003806:Dentin Permeability; D003807:Dentin Sensitivity / Q000517:prevention & control*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D019234:Dentinal Fluid; D004852:Epoxy Compounds / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D008751:Methylene Blue; D008963:Molar; D019279:Resin Cements / Q000737:chemistry; D013499:Surface Properties
chemical_list: C112274:Clearfil Liner Bond 2; D004396:Coloring Agents; D003738:Dental Cements; D003764:Dental Materials; D017220:Dentin-Bonding Agents; C122123:Ecusit-Mono; D004852:Epoxy Compounds; C445021:Gluma Comfort Bond; D008689:Methacrylates; C076808:Monobond S; C408584:Prompt L-Pop; D019279:Resin Cements; C122122:Resulcin aquaPrime; C084018:Scotchbond Multi-Purpose; C407604:Solid Bond; C425825:Solobond M; C111486:gluma desensitizer; D005976:Glutaral; D008751:Methylene Blue