title: Immunogenicity of a killed Leishmania vaccine with saponin adjuvant in dogs.
abstract: Cellular and humoral immune responses of dogs to a candidate vaccine, composed of Leishmania braziliensis promastigote protein plus saponin as adjuvant, have been investigated as a pre-requisite to understanding the mechanisms of immunogenicity against canine visceral leishmaniasis (CVL). The candidate vaccine elicited strong antigenicity related to the increases of anti-Leishmania IgG isotypes, together with higher levels of lymphocytes, particularly of circulating CD8(+) T-lymphocytes and Leishmania chagasi antigen-specific CD8(+) T-lymphocytes. As indicated by the intense cell proliferation and increased nitric oxide production during in vitro stimulation by L. chagasi soluble antigens, the candidate vaccine elicited an immune activation status potentially compatible with effective control of the etiological agent of CVL.
authors.affiliation: Laboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas/NUPEB, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil. giunchetti@nupeb.ufop.br
authors.forename: Rodolfo Cordeiro
authors.initials: RC
authors.lastname: Giunchetti
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Corrêa-Oliveira
authors.forename: Olindo Assis
authors.initials: OA
authors.lastname: Martins-Filho
authors.forename: Andréa
authors.initials: A
authors.lastname: Teixeira-Carvalho
authors.forename: Bruno Mendes
authors.initials: BM
authors.lastname: Roatt
authors.forename: Rodrigo Dian
authors.initials: RD
authors.lastname: de Oliveira Aguiar-Soares
authors.forename: Juliana Vitoriano
authors.initials: JV
authors.lastname: de Souza
authors.forename: Nádia
authors.initials: N
authors.lastname: das Dores Moreira
authors.forename: Luiz Cosme Cotta
authors.initials: LC
authors.lastname: Malaquias
authors.forename: Luciana Lisboa
authors.initials: LL
authors.lastname: Mota e Castro
authors.forename: Marta
authors.initials: M
authors.lastname: de Lana
authors.forename: Alexandre Barbosa
authors.initials: AB
authors.lastname: Reis
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001402:B-Lymphocytes / Q000276:immunology; D004283:Dog Diseases / Q000517:prevention & control*; D004285:Dogs; D005260:Female; D007892:Leishmania braziliensis / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000517:prevention & control / Q000662:veterinary*; D008213:Lymphocyte Activation; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis; D016052:Protozoan Vaccines / Q000276:immunology*; D012503:Saponins / Q000008:administration & dosage*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D015164:Vaccines, Inactivated
chemical_list: D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D016052:Protozoan Vaccines; D012503:Saponins; D015164:Vaccines, Inactivated; D009569:Nitric Oxide