title: Thermodynamics of hydrophobic interaction chromatography of acetyl amino acid methyl esters.
abstract: Thermodynamic analysis of hydrophobic interaction chromatography of amino acid methyl esters showed entropy-driven adsorption, consistent with solvophobic theory, except for phenyl ester on the Toyopearl resins. All esters adsorbed more strongly to the Toyopearl resins, including the polymethacrylate base matrix, than to Butyl Sepharose. Enthalpy changes were more favorable with the former, explaining the retention difference between Toyopearl Butyl and Butyl Sepharose. An enthalpy change versus heat capacity change plot showed Van der Waals interactions predominantly with the resin matrix. Literature data revealed the same effect for dansylamino acids, shown by isothermodynamic temperature analysis to adsorb more entropically than the esters.
authors.affiliation: Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montreal, Quebec, Canada. Gerald.Rowe@cnrc-nrc.gc.ca
authors.forename: Gerald E
authors.initials: GE
authors.lastname: Rowe
authors.forename: Hafida
authors.initials: H
authors.lastname: Aomari
authors.forename: Tatiana
authors.initials: T
authors.lastname: Chevaldina
authors.forename: Martin
authors.initials: M
authors.lastname: Lafrance
authors.forename: Sylvie
authors.initials: S
authors.lastname: St-Arnaud
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D004952:Esters / Q000737:chemistry; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D008956:Models, Chemical; D011108:Polymers / Q000737:chemistry; D012685:Sepharose / Q000031:analogs & derivatives / Q000737:chemistry; D012995:Solubility; D013696:Temperature; D013816:Thermodynamics
chemical_list: D000596:Amino Acids; D004952:Esters; D011108:Polymers; C089543:Toyopearl Phenyl 650 S; C052754:butylagarose; C044554:Toyopearl HW-65F; C058606:Toyopearl 650; D012685:Sepharose; C041725:butyl-Toyopearl 650