Effect of oral administration of Butyrivibrio fibrisolvens MDT-1, a gastrointestinal bacterium, on 3-methylcholanthrene-induced tumor in mice.
Butyrivibrio fibrisolvens MDT-1 was evaluated for use as a probiotic to prevent tumor formation. Oral administration of MDT-1 (10(9) cfu/dose, 3 times a wk for 15 wk) delayed the onset of tumors induced by 3-methylcholanthrene and also reduced tumor incidence in mice. Furthermore, the numbers of natural killer (NK) and NKT cells in the spleen increased markedly in response to MDT-1 administration. Increased numbers of NK and NKT cells may contribute to the alleviation of carcinogenesis by MDT-1 administration. Cultured splenic lymphocytes isolated from mice fed MDT-1 produced higher levels of interferon-gamma than control mice but showed no change in interleukin-4 production. These results suggest that MDT-1 administration skews the ratio of helper T (Th) cells type 1 (Th1) to Th2 toward Th1 dominance. MDT-1 administration resulted in increased immunoglobulin A secretion into the intestine. These results suggest that probiotic B. fibrisolvens MDT-1 may contribute to the prevention or alleviation of cancers, allergic disorders, and infectious diseases.
Ohkawara
Sou
S
Department of Life Science, Meiji University, Kawasaki, Japan.
Furuya
Hideki
H
Nagashima
Kousuke
K
Asanuma
Narito
N
Hino
Tsuneo
T
D000284:Administration, Oral; D000818:Animals; D045856:Butyrivibrio / Q000254:growth & development*; D015169:Colony Count, Microbial; D007111:Immunity, Cellular / Q000187:drug effects* / Q000276:immunology; D007070:Immunoglobulin A / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D016131:Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology; D008297:Male; D008748:Methylcholanthrene / Q000633:toxicity; D051379:Mice; D008813:Mice, Inbred ICR; D009369:Neoplasms / Q000517:prevention & control*; D019936:Probiotics; D011897:Random Allocation; D013154:Spleen / Q000166:cytology* / Q000276:immunology; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology
D007070:Immunoglobulin A; D015847:Interleukin-4; D008748:Methylcholanthrene; D007371:Interferon-gamma