Organic solvents-free technique for determining sulfadimethoxine and its metabolites in chicken meat.
A quick and cost-effective technique of sample preparation followed by a reversed-phase high performance liquid chromatography under "organic solvent-free" (=100% aqueous) conditions for the simultaneous quantifying of sulfadimethoxine (SDM) and its metabolites, 6-hydroxy SDM (6-OH) and N(4)-acetyl SDM (N(4)-Ac), in chicken muscle is presented. Analysis by HPLC with photo-diode array detector was performed using a short C1 column with an isocratic 0.04 mol/l citric acid mobile phase. The method was validated by the analyses of spiked chicken muscle samples, resulting recoveries (> or =84%; relative standard deviations < or =6%), analytical total time (<1/2 h/sample, where a batch of 12 samples in 4 h), and limits of quantitation (< or =0.1 microg/g). The decision limits and detection capability were 0.019-0.106 and 0.054-0.112 microg/g, respectively. No organic solvents were used at all.
Furusawa
Naoto
N
Graduate School of Human Life Science, Osaka City University, Osaka 558-8585, Japan. furusawa@life.osaka-cu.ac.jp
D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004350:Drug Residues / Q000032:analysis; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D057927:Hydrophobic and Hydrophilic Interactions; D008460:Meat / Q000032:analysis*; D011202:Poultry Products / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D013412:Sulfadimethoxine / Q000737:chemistry / Q000302:isolation & purification*
D000890:Anti-Infective Agents; D012997:Solvents; D013412:Sulfadimethoxine