Molecular typing of a suspected cluster of Nocardia farcinica infections by use of randomly amplified polymorphic DNA, pulsed-field gel electrophoresis, and amplified fragment length polymorphism analyses.
Randomly amplified polymorphic DNA (RAPD), pulsed-field gelelectrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) analyses were used to investigate a possible outbreak of Nocardia farcinica. RAPD and PFGE analyses yielded irreproducible and unsatisfactory results, respectively. AFLP analysis seem to be a promising and welcome addition for molecular analysis of Nocardia isolates.
Kalpoe
J S
JS
Department of Medical Microbiology, E4-P, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands. J.S.Kalpoe@lumc.nl
Templeton
K E
KE
Horrevorts
A M
AM
Endtz
H P
HP
Kuijper
E J
EJ
Bernards
A T
AT
Klaassen
C H W
CH
D000368:Aged; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology / Q000379:methods; D009615:Nocardia / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009617:Nocardia Infections / Q000453:epidemiology* / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique
D004269:DNA, Bacterial