title: Laboratory formulated magnetic nanoparticles for enhancement of viral gene expression in suspension cell line.
abstract: One factor critical to successful gene therapy is the development of efficient delivery systems. Although advances in gene transfer technology including viral and non-viral vectors have been made, an ideal vector system has not yet been constructed. Due to the growing concerns over the toxicity and immunogenicity of viral DNA delivery systems, DNA delivery via improve viral routes has become more desirable and advantageous. The ideal improve viral DNA delivery system should be a synthetic materials plus viral vectors. The materials should also be biocompatible, efficient, and modular so that it is tunable to various applications in both research and clinical settings. The successful steps towards this improve viral DNA delivery system is demonstrated: a magnetofection system mediated by modified cationic chitosan-coated iron oxide nanoparticles. Dense colloidal cationic iron oxide nanoparticles serve as an uptake-enhancing component by physical concentration at the cell surface in presence of external magnetic fields; enhanced viral gene expression (3-100-fold) due to the particles is seen as compared to virus vector alone with little virus dose.
authors.affiliation: Department of Bionanosystem Engineering, Chonbuk National University, Jeonju, South Korea.
authors.forename: Shanta Raj
authors.initials: SR
authors.lastname: Bhattarai
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Kyu Yun
authors.initials: KY
authors.lastname: Jang
authors.forename: Ki Chang
authors.initials: KC
authors.lastname: Lee
authors.forename: Ho Keun
authors.initials: HK
authors.lastname: Yi
authors.forename: Dae Yeol
authors.initials: DY
authors.lastname: Lee
authors.forename: Hak Yong
authors.initials: HY
authors.lastname: Kim
authors.forename: Pyoung Han
authors.initials: PH
authors.lastname: Hwang
mesh_terms: D045744:Cell Line, Tumor; D005290:Ferric Compounds; D015870:Gene Expression; D005814:Genes, Viral; D005822:Genetic Vectors; D006801:Humans; D008280:Magnetics; D053758:Nanoparticles; D014162:Transfection / Q000379:methods*
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide