Influence of culture conditions on glutathione production by Saccharomyces cerevisiae.
A strategy of experimental design using a fractional factorial design (FFD) and a central composite rotatable design (CCRD) were carried out with the aim to obtain the best conditions of temperature (20-30 degrees C), agitation rate (100-300 rpm), initial pH (5.0-7.0), inoculum concentration (5-15%), and glucose concentration (30-70 g/l) for glutathione (GSH) production in shake-flask culture by Saccharomyces cerevisiae ATCC 7754. By a FFD (2(5-2)), the agitation rate, temperature, and pH were found to be significant factors for GSH production. In CCRD (2(2)) was obtained a second-order model equation, and the percent of variation explained by the model was 95%. The results showed that the optimal culture conditions were agitation rate, 300 rpm; temperature, 20 degrees C; initial pH, 5; glucose, 54 g/l; and inoculum concentration, 5%. The highest GSH concentration (154.5 mg/l) was obtained after 72 h of fermentation.
Santos
Lucielen Oliveira
LO
Department of Food Engineering, College of Food Engineering, UNICAMP (State University of Campinas), Cx P. 6121, 13083-862,, Campinas, São Paulo, Brazil. lucielen@fea.unicamp.br
Gonzales
Tatiane Araujo
TA
Ubeda
Beatriz Torsani
BT
Monte Alegre
Ranulfo
R
D001709:Biotechnology; D003470:Culture Media; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D005978:Glutathione / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature
D003470:Culture Media; D005978:Glutathione; D005947:Glucose