Optical parameter dependence of fluorescence correlation spectrometry without using magnification tools.
The performance of fluorescence correlation spectrometry (FCS) was examined for studying the solutions suspended with the fluorescent particles of various sizes from 50 nm to 10 microm in diameter and for different sizes of pinholes: the particles were made to move by simply stirring the solution in the quartz fluorescence cuvette. Without using any magnification tool for the optical image, this FCS system successfully distinguishes images with a size of smaller than 1 microm. This system was applied for determination of the sizes of microalgae.
Fujiwara
Kitao
K
School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachiouji 192-0392, Japan.
Hirokawa
Shun
S
Aoki
Motohide
M
D010316:Particle Size; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D046939:Synechocystis / Q000166:cytology*