Circulating heat shock proteins and inflammatory markers in patients with idiopathic left ventricular dysfunction: their relationships with myocardial and microvascular impairment.
Little information is available on peripheral levels of Hsp72, Hsp60, and anti-Hsp60 antibodies in patients with left ventricular (LV) dysfunction due to non-atherosclerotic cardiac disease. In this study, serum Hsp72, Hsp60 and anti-Hsp60 antibodies, IL-6, and C-reactive protein (CRP) were measured in 44 healthy controls and in 82 patients with angiographically normal coronary arteries (LV ejection fraction [EF] > or = 50%, n=22; -35% to <50%, n=32; <35%, n=28). Patients with more severe disease (more depressed myocardial blood flow at rest and during dipyridamole, indicative of coronary microvascular impairment) showed more elevated circulating Hsp60 and auto-antibodies, Hsp72, and CRP levels. IL-6 was increased progressively as a function of severity of LV dysfunction. Anti-Hsp60 antibodies, Hsp72, and IL-6 were significantly correlated with brain natriuretic peptide (BNP) levels and LV end-diastolic dimensions (LVEDD) values. IL-6 tended to be related with Hsp72 in particular in patients with more severe disease (r = 0.45, P = 0.021). Hsp60 and Hsp72 activation and inflammatory markers were correlated with the extent of cardiac and microvascular dysfunction in patients with angiographycally normal coronary arteries. These results suggest a pathogenic role of infective-metabolic insult and inflammatory reaction in the development of vascular and myocardial damage in patients with heart failure even in the absence of overt coronary artery disease.
Giannessi
Daniela
D
Consiglio Nazionale delle Ricerche Institute of Clinical Physiology, Laboratory of Cardiovascular Biochemistry, Pisa 56100, Italy. danielag@ifc.cnr.it
Colotti
Chiara
C
Maltinti
Maristella
M
Del Ry
Silvia
S
Prontera
Concetta
C
Turchi
Stefano
S
Labbate
Antonio
A
Neglia
Danilo
D
D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism*; D002303:Cardiac Output, Low / Q000097:blood / Q000150:complications* / Q000503:physiopathology; D018834:Chaperonin 60 / Q000097:blood* / Q000276:immunology; D003326:Coronary Circulation; D005260:Female; D050884:HSP72 Heat-Shock Proteins / Q000097:blood*; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D008297:Male; D008833:Microcirculation / Q000503:physiopathology; D008875:Middle Aged; D009200:Myocardial Contraction; D020097:Natriuretic Peptide, Brain / Q000097:blood; D012720:Severity of Illness Index; D018487:Ventricular Dysfunction, Left / Q000097:blood* / Q000209:etiology / Q000276:immunology / Q000503:physiopathology
D001323:Autoantibodies; D018834:Chaperonin 60; D050884:HSP72 Heat-Shock Proteins; C508600:IL6 protein, human; D018836:Inflammation Mediators; D015850:Interleukin-6; D020097:Natriuretic Peptide, Brain; D002097:C-Reactive Protein