title: A chronically implantable device for the controlled delivery of substances, and stimulation and recording of activity in severed nerves.
abstract: We describe the use of an implantable device for peripheral nerves that allows chronic simultaneous delivery of small volumes of solution, recording of both field and multiunit potentials, and electrical stimulation. This custom-made multifunctional device was attached to the cut end of the abducens (VIth) nerve for stimulation, recording and injection purposes. Our device consists of a polyethylene chamber with two electrodes that can be used for stimulation and recording and two Teflon tubes that serve as inlet and outlet for administering chemicals to the nerve fitted inside. Since the device is implanted in a retro-orbital position, we herein will refer to it as an intraorbitary device (IOD). The applicability of the IOD is demonstrated with an electrophysiological and anatomical account of the properties of the abducens nerve. Furthermore, it is shown that certain neuronal discharge properties can be inferred from the nerve recordings. The IOD can also be efficiently used for the delivery of small volume of pharmacological substances or conventional retrograde markers.
authors.affiliation: Departamento de Fisiología y Zoología, Facultad de Biología, Universidad de Sevilla, 41012 Sevilla, Spain.
authors.forename: María A
authors.initials: MA
authors.lastname: Davis-López de Carrizosa
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Tena
authors.forename: Beatriz
authors.initials: B
authors.lastname: Benítez-Temiño
authors.forename: Camilo J
authors.initials: CJ
authors.lastname: Morado-Díaz
authors.forename: Angel M
authors.initials: AM
authors.lastname: Pastor
authors.forename: Rosa R
authors.initials: RR
authors.lastname: de la Cruz
mesh_terms: D020222:Abducens Nerve Injury / Q000473:pathology / Q000503:physiopathology*; D000200:Action Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D000818:Animals; D002415:Cats; D004307:Dose-Response Relationship, Radiation; D016503:Drug Delivery Systems / Q000295:instrumentation*; D004558:Electric Stimulation / Q000295:instrumentation* / Q000379:methods*; D005456:Fluorescent Dyes / Q000008:administration & dosage; D009046:Motor Neurons / Q000187:drug effects / Q000528:radiation effects; D019736:Prostheses and Implants
chemical_list: D005456:Fluorescent Dyes