title: HPLC and chemometrics-assisted UV-spectroscopy methods for the simultaneous determination of ambroxol and doxycycline in capsule.
abstract: High-performance liquid chromatography (HPLC) and multivariate spectrophotometric methods are described for the simultaneous determination of ambroxol hydrochloride (AM) and doxycycline (DX) in combined pharmaceutical capsules. The chromatographic separation was achieved on reversed-phase C(18) analytical column with a mobile phase consisting of a mixture of 20mM potassium dihydrogen phosphate, pH 6-acetonitrile in ratio of (1:1, v/v) and UV detection at 245 nm. Also, the resolution has been accomplished by using numerical spectrophotometric methods as classical least squares (CLS), principal component regression (PCR) and partial least squares (PLS-1) applied to the UV spectra of the mixture and graphical spectrophotometric method as first derivative of the ratio spectra ((1)DD) method. Analytical figures of merit (FOM), such as sensitivity, selectivity, analytical sensitivity, limit of quantitation and limit of detection were determined for CLS, PLS-1 and PCR methods. The proposed methods were validated and successfully applied for the analysis of pharmaceutical formulation and laboratory-prepared mixtures containing the two component combination.
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt. ghhadad@hotmail.com
authors.forename: Ghada M
authors.initials: GM
authors.lastname: Hadad
authors.forename: Alaa
authors.initials: A
authors.lastname: El-Gindy
authors.forename: Waleed M M
authors.initials: WM
authors.lastname: Mahmoud
mesh_terms: D000551:Ambroxol / Q000032:analysis*; D000704:Analysis of Variance; D002138:Calibration; D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004318:Doxycycline / Q000032:analysis*; D016018:Least-Squares Analysis; D004364:Pharmaceutical Preparations; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D002214:Capsules; D004364:Pharmaceutical Preparations; D012996:Solutions; D000551:Ambroxol; D004318:Doxycycline