Isolation and identification of Enterococcus faecalis from necrotic root canals using multiplex PCR.
This study was designed to survey the incidence of Enterococcus faecalis infection in symptomatic and asymptomatic root canals of necrotic teeth using PCR and to isolate the bacterium for further screening. Sixty patients categorized according to their clinical symptoms were used for sampling by insertion of paper points into the root canals and absorbing all the fluids present within them. The samples were incubated in 1.0 ml 2xYT (containing 16 g bacto tryptone, 10 g yeast extract and 5.0 g NaCl per liter) for 24 h at 37 degrees C without aeration prior to multiplex PCR analysis. To assist the isolation of E. faecalis, sub-samples were further grown in the same medium supplemented with 6.5% NaCl and back-inoculated into bile esculin. Using multiple cultivation-dependent and PCR analyses, 6 cases (10%) of E. faecalis were identified. Four isolates were obtained from asymptomatic cases of chronic apical periodontitis, and the other two were associated with phoenix abscess and acute apical abscess, respectively. No E. faecalis infection was found in 5 patients with acute apical periodontitis or in 9 with chronic suppurative periodontitis. Our results indicate that there is no significant difference in the incidence of E. faecalis between symptomatic and asymptomatic necrotic dental root canals (P > 0.05).
Mahmoudpour
Ali
A
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz Iran. ali5491338@yahoo.com
Rahimi
Saeed
S
Sina
Mahmood
M
Soroush
Mohammad H
MH
Shahi
Shahriar
S
Shahisa
Shahriar
S
Asl-Aminabadi
Naser
N
D000293:Adolescent; D000328:Adult; D015373:Bacterial Typing Techniques; D002648:Child; D004269:DNA, Bacterial / Q000032:analysis; D003790:Dental Pulp Necrosis / Q000382:microbiology*; D004587:Electrophoresis, Agar Gel; D013293:Enterococcus faecalis / Q000302:isolation & purification*; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010485:Periapical Periodontitis / Q000382:microbiology; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial