[Optimization of extraction parameters and de-protein process for Gastrodia elata. Polysaccharides].
OBJECTIVE
Grope for Optimization of Extraction Parameters and De-protein Process for Gastrodia elata Bl. Polysaccharides (GPS). Provide the theoretical basis for the industrialization production of GPS.


METHODS
The effects of extracting temperature, ration between Gastrodia elata Bl. and water, heating time on extraction rate of GPS were studied with orthogonal experimental design. Compared the application of the two de-protein process measures (Sevag and Enzyme-Sevag) in purification technics for Gastrodia elata Bl.


RESULTS
The optimum parameters were: extraction 3h at 120 degrees C with 1:40 ratio of Gastrodia elata Bl. to water. The de-protein efficiency of Enzyme-Sevag is better than Sevag.
Zhu
Xiao-Xia
XX
Southwest University of Science and Technology, Mianyang 621010, China.
Luo
Xue-Gang
XG
D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D031668:Gastrodia / Q000737:chemistry*; D035281:Plant Tubers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D011134:Polysaccharides