title: Complex carbohydrate specificity of lectin from fruiting body of Ganoderma lucidum. A surface plasmon resonance study.
abstract: The thermodynamics and kinetics of binding of glycans and glycoproteins to Ganoderma lucidum lectin was studied using surface plasmon resonance. The lectin showed highest affinity for asialo triantennary N glycan (Ka = 3.52 x 10(5)) among the glycans tested. There was a several fold increase in affinity for glycoproteins compared to their corresponding glycans and coincident increase in contribution from enthalpy (DeltaH), suggesting the involvement of hydrogen bonding in the interaction as well as involvement of protein-protein interactions. Increased affinity also showed increase in unfavorable negative binding entropy (DeltaS) which was compensated with higher enthalpy. The glycoproteins showed faster association rates (k(1)) and the activation energy (E(1)) in the association process was much lower for the glycoproteins than glycans, resulting in their faster associations. These observations elaborate the role of protein matrix in lectin-glycoconjugate interaction.
authors.affiliation: Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.
authors.forename: Atul
authors.initials: A
authors.lastname: Thakur
authors.forename: Lakhan
authors.initials: L
authors.lastname: Pal
authors.forename: Absar
authors.initials: A
authors.lastname: Ahmad
authors.forename: M I
authors.initials: MI
authors.lastname: Khan
mesh_terms: D015374:Biosensing Techniques; D005340:Fibrinogen / Q000378:metabolism; D006001:Glycoconjugates / Q000737:chemistry / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism; D006860:Hydrogen Bonding; D007700:Kinetics; D037102:Lectins / Q000302:isolation & purification / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D020940:Reishi / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D013816:Thermodynamics; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: D006001:Glycoconjugates; D006023:Glycoproteins; D037102:Lectins; D011134:Polysaccharides; D000509:alpha-Fetoproteins; D005340:Fibrinogen