title: [Determination of alpha-glucosidase activity in seminal plasma by semi-automatic biochemistry analyzer].
abstract: OBJECTIVE
  To establish a method of determining alpha-glucosidase activity in seminal plasma by semi-automatic biochemistry analyzer.
  
  
  METHODS
  Alpha-glucosidase activity in seminal plasma from 51 men with normal semen parameters in routine semen analysis were detected by semi-automatic biochemistry analyzer and manual glucose oxidase method, respectively. Then, the intra-assay and inter-assay coefficient variation (CV) and normal reference value were calculated. In the meanwhile, the correlation between the two methods was analyzed.
  
  
  RESULTS
  The intra-assay CVs of 2 seminal plasma samples with different alpha-glucosidase activity detected by semi-automatic biochemistry analyzer were 12.63% and 9.13%, and the inter-assay CVs were 10.67% and 13.49%, respectively. The normal reference value for seminal alpha-glucosidase activity detected with semi-automatic biochemistry analyzer ranged from 102.28 to 555.08 U/L. There was a significantly positive correlation between the semi-automatic biochemistry analyzer and the manual glucose oxidase method (r = 0.792, P < 0.01).
  
  
  CONCLUSIONS
  The method of determining alpha-glucosidase activity in seminal plasma by semi-automatic biochemistry analyzer, with its simplicity, less cost of time and reagents, and more reliable result, could be applied to clinical laboratory medicine.
authors.affiliation: Department of Reproduction & Genetics, PLA Institute of Clinical Laboratory Medicine, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China. jclu@jlonline.com
authors.forename: Jin-chun
authors.initials: JC
authors.lastname: Lu
authors.forename: Hui-ru
authors.initials: HR
authors.lastname: Xu
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Huang
mesh_terms: D000328:Adult; D001671:Biochemistry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008297:Male; D012016:Reference Values; D015203:Reproducibility of Results; D012661:Semen / Q000201:enzymology*; D000520:alpha-Glucosidases / Q000032:analysis / Q000378:metabolism*
chemical_list: D000520:alpha-Glucosidases