Side population cells expressing ABCG2 in human adult dental pulp tissue.
OBJECTIVE
To investigate the presence of side population (SP) cells by the Hoechst exclusion method in human adult dental pulp tissue.


METHODS
Human adult dental pulp-derived cells were generated from third molar teeth. The cells were stained with Hoechst 33342 and sorted into SP cells or non-SP cells [main population (MP) cells]. Both cell types were compared with cell growth and RT-PCR analyses.


RESULTS
SP cells that express ABCG2, Nestin, Notch-1 and alpha-smooth muscle actin were found at frequencies ranging from 0.67% to 1.02%. This SP profile disappeared in the presence of verapamil. These SP cells expressed dentine sialophosphoprotein and dentine matrix protein-1 when cultured in osteogenic medium.


CONCLUSIONS
Human adult dental pulp tissue contains SP cells that differentiate into odontoblast-like cells.
Honda
M J
MJ
Tooth Regeneration, Division of Stem Cell Engineering, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan. honda-m@ims.u-tokyo.ac.jp
Nakashima
F
F
Satomura
K
K
Shinohara
Y
Y
Tsuchiya
S
S
Watanabe
N
N
Ueda
M
M
D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis* / Q000494:pharmacology; D000199:Actins / Q000096:biosynthesis; D000293:Adolescent; D000328:Adult; D001562:Benzimidazoles; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005456:Fluorescent Dyes; D015870:Gene Expression; D006801:Humans; D007381:Intermediate Filament Proteins / Q000096:biosynthesis; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D064231:Nestin; D009804:Odontoblasts / Q000378:metabolism*; D010750:Phosphoproteins / Q000096:biosynthesis; D051881:Receptor, Notch1 / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism*; D014700:Verapamil / Q000494:pharmacology; D000099311:Dentin Sialophosphoprotein
D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D000199:Actins; D001562:Benzimidazoles; D016326:Extracellular Matrix Proteins; D005456:Fluorescent Dyes; D007381:Intermediate Filament Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D064231:Nestin; D010750:Phosphoproteins; D051881:Receptor, Notch1; D012795:Sialoglycoproteins; D014700:Verapamil; D000099311:Dentin Sialophosphoprotein; C116614:ABCG2 protein, human; C513309:DMP1 protein, human; C577484:NES protein, human; C017807:bisbenzimide ethoxide trihydrochloride