title: Crystal structure of homoserine O-acetyltransferase from Leptospira interrogans.
abstract: Homoserine O-acetyltransferase (HTA, EC 2.3.1.31) initiates methionine biosynthesis pathway by catalyzing the transfer of acetyl group from acetyl-CoA to homoserine. This study reports the crystal structure of HTA from Leptospira interrogans determined at 2.2A resolution using selenomethionyl single-wavelength anomalous diffraction method. HTA is modular and consists of two structurally distinct domains--a core alpha/beta domain containing the catalytic site and a helical bundle called the lid domain. Overall, the structure fold belongs to alpha/beta hydrolase superfamily with the characteristic 'catalytic triad' residues in the active site. Detailed structure analysis showed that the catalytic histidine and serine are both present in two conformations, which may be involved in the catalytic mechanism for acetyl transfer.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, PR China.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Yanli
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Wei
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Yikun
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Jiang
authors.forename: Hang
authors.initials: H
authors.lastname: Xu
authors.forename: Weimin
authors.initials: W
authors.lastname: Gong
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001665:Binding Sites; D018360:Crystallography, X-Ray; D019281:Dimerization; D015870:Gene Expression; D006639:Histidine / Q000235:genetics / Q000378:metabolism; D007921:Leptospira interrogans / Q000201:enzymology* / Q000235:genetics; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D012694:Serine / Q000235:genetics / Q000378:metabolism; D040681:Structural Homology, Protein
chemical_list: D012694:Serine; D006639:Histidine; D000123:Acetyltransferases; C021369:homoserine O-acetyltransferase