title: Ribulose-1,5-bisphosphate carboxylase/oxygenase from thermophilic cyanobacterium Thermosynechococcus elongatus.
abstract: Ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) can be divided into two branches: the "red-like type" of marine algae and the "green-like type" of cyanobacteria, green algae, and higher plants. We found that the "green-like type" rubisco from the thermophilic cyanobacterium Thermosynechococcus elongatus has an almost 2-fold higher specificity factor compared with rubiscos of mesophilic cyanobacteria, reaching the values of higher plants, and simultaneously revealing an improvement in enzyme thermostability. The difference in the activation energies at the transition stages between the oxygenase and carboxylase reactions for Thermosynechococcus elongatus rubisco is very close to that of Galdieria partita and significantly higher than that of spinach. This is the first characterization of a "green-like type" rubisco from thermophilic organism.
authors.affiliation: Department of Biotechnology, University of Wroclaw, Przybyszewskiego 63/77, Wroclaw, 51-148, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Gubernator
authors.forename: Rafal
authors.initials: R
authors.lastname: Bartoszewski
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Kroliczewski
authors.forename: Guenter
authors.initials: G
authors.lastname: Wildner
authors.forename: Andrzej
authors.initials: A
authors.lastname: Szczepaniak
mesh_terms: D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D007700:Kinetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D012273:Ribulose-Bisphosphate Carboxylase