title: Association of body mass index and Trp64Arg polymorphism of the beta3-adrenoreceptor gene and leptin level in Tehran Lipid and Glucose Study.
abstract: In this study the association between beta3-adrenoceptor gene polymorphism and serum concentration of leptin with body mass index (BMI) is investigated. Using subjects in the Tehran Lipid and Glucose Study, genotyping of the Trp64Arg polymorphism of the beta3-adrenoreceptor gene was performed using a restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) technique was used and the association with obesity was investigated. At total of 197 men and 204 women were divided into four groups (BMI<20, 20< or =BMI<25, 25< or =BMI<30, BMI< or =30) and 97, 98, 104 and 102 subjects, respectively, were placed randomly in the four groups. Leptin level was determined by an enzyme immunoassay (EIA) method and FBS, HDL-C, triglyceride and total cholesterol levels were determined by an enzyme colorimetric method. Body mass index (BMI) was also measured. The A (Arg) allele frequency was 0.08 among the population and its presence was significantly associated with increase of leptin level (AA/TA, 30.5+/-24.8 ng/mL; TT, 22.6+/-20.9 ng/mL; P=0.014) but there was no significant association with increased BMI (AA/TA, 27+/-5.6 kg/m2; TT, 25.4+/-5.5 kg/m2; P=0.072). These data show that the presence of the Arg64 allele at the beta3-adenoceptor gene locus is related to increase in leptin level in this population, but is not related to body mass index.
authors.affiliation: Institute of Endocrine Sciences, Shaheed Beheshti University of Medical Sciences, Tehran, I. R. Iran.
authors.forename: P
authors.initials: P
authors.lastname: Eshraghi
authors.forename: M
authors.initials: M
authors.lastname: Hedayati
authors.forename: M S
authors.initials: MS
authors.lastname: Daneshpour
authors.forename: P
authors.initials: P
authors.lastname: Mirmiran
authors.forename: F
authors.initials: F
authors.lastname: Azizi
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D005260:Female; D006801:Humans; D007492:Iran / Q000453:epidemiology; D020738:Leptin / Q000378:metabolism*; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood* / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics*; D022702:Receptors, Adrenergic, beta-3 / Q000235:genetics*; D054411:Receptors, Leptin / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D020738:Leptin; D008055:Lipids; D022702:Receptors, Adrenergic, beta-3; D054411:Receptors, Leptin