title: Mammalian Sir2 homolog SIRT3 regulates global mitochondrial lysine acetylation.
abstract: Homologs of the Saccharomyces cerevisiae Sir2 protein, sirtuins, promote longevity in many organisms. Studies of the sirtuin SIRT3 have so far been limited to cell culture systems. Here, we investigate the localization and function of SIRT3 in vivo. We show that endogenous mouse SIRT3 is a soluble mitochondrial protein. To address the function and relevance of SIRT3 in the regulation of energy metabolism, we generated and phenotypically characterized SIRT3 knockout mice. SIRT3-deficient animals exhibit striking mitochondrial protein hyperacetylation, suggesting that SIRT3 is a major mitochondrial deacetylase. In contrast, no mitochondrial hyperacetylation was detectable in mice lacking the two other mitochondrial sirtuins, SIRT4 and SIRT5. Surprisingly, despite this biochemical phenotype, SIRT3-deficient mice are metabolically unremarkable under basal conditions and show normal adaptive thermogenesis, a process previously suggested to involve SIRT3. Overall, our results extend the recent finding of lysine acetylation of mitochondrial proteins and demonstrate that SIRT3 has evolved to control reversible lysine acetylation in this organelle.
authors.affiliation: Howard Hughes Medical Institute, The Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: David B
authors.initials: DB
authors.lastname: Lombard
authors.forename: Frederick W
authors.initials: FW
authors.lastname: Alt
authors.forename: Hwei-Ling
authors.initials: HL
authors.lastname: Cheng
authors.forename: Jakob
authors.initials: J
authors.lastname: Bunkenborg
authors.forename: Ryan S
authors.initials: RS
authors.lastname: Streeper
authors.forename: Raul
authors.initials: R
authors.lastname: Mostoslavsky
authors.forename: Jennifer
authors.initials: J
authors.lastname: Kim
authors.forename: George
authors.initials: G
authors.lastname: Yancopoulos
authors.forename: David
authors.initials: D
authors.lastname: Valenzuela
authors.forename: Andrew
authors.initials: A
authors.lastname: Murphy
authors.forename: Yinhua
authors.initials: Y
authors.lastname: Yang
authors.forename: Yaohui
authors.initials: Y
authors.lastname: Chen
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Hirschey
authors.forename: Roderick T
authors.initials: RT
authors.lastname: Bronson
authors.forename: Marcia
authors.initials: M
authors.lastname: Haigis
authors.forename: Leonard P
authors.initials: LP
authors.lastname: Guarente
authors.forename: Robert V
authors.initials: RV
authors.lastname: Farese
authors.forename: Sherman
authors.initials: S
authors.lastname: Weissman
authors.forename: Eric
authors.initials: E
authors.lastname: Verdin
authors.forename: Bjoern
authors.initials: B
authors.lastname: Schwer
mesh_terms: D000107:Acetylation; D002001:Adipose Tissue, Brown / Q000166:cytology; D000818:Animals; D005247:Feeding Behavior; D005508:Food Deprivation; D018390:Gene Targeting; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D006655:Histone Deacetylases / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D008322:Mammals / Q000378:metabolism*; D051379:Mice; D008930:Mitochondria, Liver / Q000201:enzymology / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000172:deficiency / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae / Q000378:metabolism*; D056565:Sirtuin 2; D056566:Sirtuin 3; D037761:Sirtuins / Q000172:deficiency / Q000378:metabolism*; D012995:Solubility; D022722:Thermogenesis
chemical_list: D024101:Mitochondrial Proteins; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; C525046:Sirt3 protein, mouse; D005969:Glutamate Dehydrogenase; C087778:SIR2 protein, S cerevisiae; D056565:Sirtuin 2; D056566:Sirtuin 3; D037761:Sirtuins; D006655:Histone Deacetylases; D008239:Lysine