title: Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of zearalenone and deoxynivalenol.
abstract: A multianalyte lateral-flow technique using colloidal gold-labeled monoclonal antibodies was developed for the rapid simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEA). The results of this qualitative one-step test were interpreted visually. A very simple and fast sample preparation was used, and the assay procedure could be accomplished within 10 min. When applied to spiked wheat samples, the technique gave accurate and reproducible results. Cut-off levels of 1500 and 100 microg kg(-1) for DON and ZEA, respectively, were observed. The described multianalyte format can be used as a reliable, rapid and cost-effective on-site screening technique for the simultaneous determination of mycotoxins in grain samples.
authors.affiliation: Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium. anna_kolosova@hotmail.com
authors.forename: Anna Yu
authors.initials: AY
authors.lastname: Kolosova
authors.forename: Sarah
authors.initials: S
authors.lastname: De Saeger
authors.forename: Liberty
authors.initials: L
authors.lastname: Sibanda
authors.forename: Ron
authors.initials: R
authors.lastname: Verheijen
authors.forename: Carlos
authors.initials: C
authors.lastname: Van Peteghem
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D003102:Colloids / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D013997:Time Factors; D014255:Trichothecenes / Q000032:analysis* / Q000737:chemistry; D015025:Zearalenone / Q000032:analysis* / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D003102:Colloids; D014255:Trichothecenes; D015025:Zearalenone; D006046:Gold; C007262:deoxynivalenol