title: Metabolic rate and reactive oxygen species production in different genotypes of GH-transgenic zebrafish.
abstract: Growth hormone overexpression increases growth and consequently increases the metabolic rate in fishes. Therefore, the objective of this study was to evaluate the effects of growth hormone overexpression in zebrafish Danio rerio in terms of growth, oxygen consumption, reactive oxygen species production, lipid hydroperoxide content, antioxidant enzyme activity and glutamate-cysteine ligase catalytic subunit gene expression. The employed models were wild type and transgenic (hemizygous and homozygous) zebrafish expressing the Odonthestes argentinensis growth hormone gene directed by the Cyprinus carpio beta-actin promoter. Higher growth parameters were observed in the hemizygous group. The homozygous group possessed higher oxygen consumption and reactive oxygen species production. Growth hormone transgenesis causes a decrease in glutamate-cysteine ligase catalytic subunit expression, an enzyme responsible for glutathione synthesis. Although the lipid hydroperoxide content was similar between groups, we demonstrate that growth hormone overexpression has the potential to generate oxidative stress in fishes.
authors.affiliation: Fundação Universidade Federal do Rio Grande, Brazil. ninovski@octopus.furg.br
authors.forename: C E
authors.initials: CE
authors.lastname: Rosa
authors.forename: M A
authors.initials: MA
authors.lastname: Figueiredo
authors.forename: C F C
authors.initials: CF
authors.lastname: Lanes
authors.forename: D V
authors.initials: DV
authors.lastname: Almeida
authors.forename: J M
authors.initials: JM
authors.lastname: Monserrat
authors.forename: L F
authors.initials: LF
authors.lastname: Marins
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism; D020134:Catalytic Domain / Q000235:genetics; D005721:Glutamate-Cysteine Ligase / Q000096:biosynthesis* / Q000235:genetics; D005978:Glutathione / Q000096:biosynthesis / Q000235:genetics; D013006:Growth Hormone / Q000096:biosynthesis* / Q000235:genetics; D015227:Lipid Peroxidation / Q000235:genetics*; D010101:Oxygen Consumption / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000199:Actins; D000975:Antioxidants; D017382:Reactive Oxygen Species; D029961:Zebrafish Proteins; D013006:Growth Hormone; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione