A new polymerase chain reaction/restriction fragment length polymorphism protocol for Plasmodium vivax circumsporozoite protein genotype (VK210, VK247, and P. vivax-like) determination.
For the molecular diagnosis of Plasmodium vivax variants (VK210, VK247, and P. vivax-like) using DNA amplification procedures in the laboratory, the choice of rapid and inexpensive identification products of the 3 different genotypes is an important prerequisite. We report here the standardization of a new polymerase chain reaction/restriction fragment length polymorphism technique to identify the 3 described P. vivax circumsporozoite protein (CSP) variants using amplification of the central immunodominant region of the CSP gene of this protozoan. The simplicity, specificity, and sensitivity of the system described here is important to determine the prevalence and the distribution of infection with these P. vivax genotypes in endemic and nonendemic malaria areas, enabling a better understanding of their phylogeny.
Alves
Renata Tomé
RT
Universidade Estadual Paulista Júlio de Mesquita Filho, 15054-000 São José do Rio Preto, São Paulo State, Brazil.
Póvoa
Marinete Marins
MM
Goldman
Ira F
IF
Cavasini
Carlos Eugênio
CE
Rossit
Andréa Regina Baptista
AR
Machado
Ricardo Luiz Dantas
RL
D000818:Animals; D005838:Genotype; D006801:Humans; D016780:Malaria, Vivax / Q000469:parasitology*; D010274:Parasitology / Q000379:methods; D010966:Plasmodium vivax / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D015800:Protozoan Proteins / Q000235:genetics*; D012680:Sensitivity and Specificity
D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan