title: No evidence of linkage between 7q33-36 locus (OTSC2) and otosclerosis in seven British Caucasian pedigrees.
abstract: BACKGROUND
  The aetiology of otosclerosis is complex, and probably involves an interaction between genes and environmental factors. Previous studies have revealed genetic linkage with a number of chromosome regions, including position 7q33-36.
  
  
  OBJECTIVE
  To confirm whether linkage exists between otosclerosis and chromosome region 7q33-36.
  
  
  METHODS
  Seven multiply affected families were ascertained. Deoxyribonucleic acid from members of these families was extracted, and six markers were genotyped to cover a 16 cM region at 7q33-36. Both parametric and non-parametric multipoint linkage analyses were performed.
  
  
  RESULTS
  Parametric multipoint linkage analysis excluded any linkage at 7q33-36 (logarithm of odds score <-4.0). Non-parametric linkage analysis also failed to confirm any linkage (non-parametric linkage < 1.66). When tested individually, pedigree four was the only one to show a significant non-parametric linkage score between D7s684 and D7s2513 (non-parametric linkage = 1.96).
  
  
  CONCLUSIONS
  No linkage was detected between otosclerosis and the 7q33-36 region. This could be explained by the study's lack of power, due to the limited number of families available.
authors.affiliation: Otolaryngology Department, Jordan University of Science and Technology, Irbid, Jordan.
authors.forename: F Q
authors.initials: FQ
authors.lastname: Alzoubi
authors.forename: W R
authors.initials: WR
authors.lastname: Ollier
authors.forename: R T
authors.initials: RT
authors.lastname: Ramsden
authors.forename: S R
authors.initials: SR
authors.lastname: Saeed
mesh_terms: D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D006801:Humans; D008126:Lod Score; D008297:Male; D018895:Microsatellite Repeats; D010040:Otosclerosis / Q000235:genetics*; D010375:Pedigree