title: Electrospray ionization and time-of-flight mass spectrometric method for simultaneous determination of spermidine and spermine.
abstract: A sensitive method for the determination of polyamines in mammalian cells was described using electrospray ionization and time-of-flight mass spectrometer. This method was 50-fold more sensitive than the previous method using ionspray ionization and quadrupole mass spectrometer. The method employed the partial purification and derivatization of polyamines, but allowed a measurement of multiple samples which contained picomol amounts of polyamines. Time required for data acquisition of one sample was approximately 2 min. The method was successfully applied for the determination of reduced spermidine and spermine contents in cultured cells under the inhibition of aminopropyltransferases. In addition, a new proper internal standard was proposed for the tracer experiment using (15)N-labeled polyamines.
authors.affiliation: Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo, Japan. samejima@musashino-u.ac.jp
authors.forename: Keijiro
authors.initials: K
authors.lastname: Samejima
authors.forename: Masahiro
authors.initials: M
authors.lastname: Otani
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Murakami
authors.forename: Takami
authors.initials: T
authors.lastname: Oka
authors.forename: Misao
authors.initials: M
authors.lastname: Kasai
authors.forename: Hiroki
authors.initials: H
authors.lastname: Tsumoto
authors.forename: Kohfuku
authors.initials: K
authors.lastname: Kohda
mesh_terms: D002460:Cell Line; D002477:Cells / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003514:Cyclohexylamines / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D013058:Mass Spectrometry; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization; D013095:Spermidine / Q000032:analysis*; D013096:Spermine / Q000032:analysis*
chemical_list: D003514:Cyclohexylamines; D004791:Enzyme Inhibitors; D011506:Proteins; D013096:Spermine; C059116:4-methylcyclohexylamine; D013095:Spermidine