Activation of T-cells from allergic patients and volunteers by p-phenylenediamine and Bandrowski's base.
Allergic contact dermatitis is commonly associated with exposure to p-phenylenediamine. The aim of this study was to determine whether p-phenylenediamine (PPD) and/or Bandrowski's base (BB) stimulate T cells from allergic patients and volunteers, and to explore the relationship between T-cell immunogenicity and allergy. Lymphocytes from allergic patients proliferated with PPD and BB (n=8). Lymphocytes from 14/16 non-allergic individuals also proliferated following stimulation, but only with BB; cord blood lymphocytes failed to respond (n=6). Glutathione, which prevented BB formation, but not binding of PPD to cells and serum, did not prevent p-phenylenediamine-specific stimulation of patient lymphocytes. T-cell clones generated from allergic patients were stimulated separately with PPD and BB, while clones from volunteers proliferated with BB alone. Patient and volunteer clones secreted IL-4, IL-5, IL-13, TNF-alpha, MIP-1alpha, MIP-1beta, and RANTES. These data show that activation of T lymphocytes from allergic individuals alone with PPD represents an important discrimination between allergic and non-allergic groups. BB-specific T cells are found in both allergic patients and volunteers, but not in cord blood. Their presence seems to reflect an acquired immune response, which is not translated into an allergic reaction.
Coulter
Eve Marie
EM
Department of Pharmacology, The University of Liverpool, Liverpool, UK.
Jenkinson
Claire
C
Wu
Ying
Y
Farrell
John
J
Foster
Brian
B
Smith
Andrew
A
McGuire
Carolann
C
Pease
Camilla
C
Basketter
David
D
King
Clodagh
C
Friedmann
Peter Simon
PS
Pirmohamed
Munir
M
Park
Brian Kevin
BK
Naisbitt
Dean J
DJ
D000328:Adult; D000368:Aged; D015704:CD4 Antigens / Q000187:drug effects / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D017449:Dermatitis, Allergic Contact / Q000276:immunology*; D005260:Female; D006202:Hair Dyes / Q000009:adverse effects* / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D010655:Phenylenediamines / Q000276:immunology* / Q000494:pharmacology
D015704:CD4 Antigens; D016207:Cytokines; D006202:Hair Dyes; D010655:Phenylenediamines; C051597:Bandrowski's base; C029728:4-phenylenediamine