title: Complete genomic RNA sequence of the Polish Pepino mosaic virus isolate belonging to the US2 strain.
abstract: We have determined the complete nucleotide and amino acid sequences of the Polish Pepino mosaic virus (PepMV) isolate marked as PepMV-PK. The PepMV-PK genome consists of a single positive-sense RNA strand of 6412-nucleotide-long that contains five open reading frames (ORFs). ORF1 encodes the putative viral polymerase (RdRp), ORFs 2-4 the triple gene block (TGB 1-3), and ORF5-coat protein CP. Two short untranslated regions flank the coding ones and there is a poly (A) tail at the 3' end of the genomic RNA. Thus, the genome organization of PepMV-PK is that of a typical member of the genus Potexvirus. Phylogenetic analysis based on full-length genomes of PepMV sequences showed that PepMV-PK was most closely related to the Ch2 isolate from Chile. Comparison of PepMV-PK and Ch2 showed the following nucleotide identities: 98% for the RdRp, 99% for the CP genes, and 98, 99, and 98% for the TGB1, TGB2, and TBG3, respectively. This high level of nucleotide sequence identity between the Chilean and Polish PepMV-PK isolates suggest their common origin.
authors.affiliation: Department of Virology and Bacteriology, Institute of Plant Protection, Miczurina 20, 60-318 Poznan, Poland. B.Hasiow@ior.poznan.pl
authors.forename: Beata
authors.initials: B
authors.lastname: Hasiów
authors.forename: Natasza
authors.initials: N
authors.lastname: Borodynko
authors.forename: Henryk
authors.initials: H
authors.lastname: Pospieszny
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002677:Chile; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D011044:Poland; D020641:Polymorphism, Single Nucleotide; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins