Growth and cometabolic reduction kinetics of a uranium- and sulfate-reducing Desulfovibrio/Clostridia mixed culture: Temperature effects.
Bioremediation of contaminated soils and aquifers is subject to spatial and temporal temperature changes that can alter the kinetics of key microbial processes. This study quantifies temperature effects on the kinetics of an ethanol-fed sulfate-reducing mixed culture derived from a uranium-contaminated aquifer subject to seasonal temperature fluctuations. The mixed culture contains Desulfovibrio sp. and a Clostridia-like organism. Rates of growth, ethanol utilization, decay, and uranium reduction decreased with decreasing temperature. No significant uranium reduction was observed at 10 degrees C. While both Monod saturation kinetics and pseudo second-order kinetics adequately described the rates of growth and utilization of electron donor (ethanol), model parameters for the pseudo second-order expression had smaller uncertainties. Uranium reduction kinetics were best described by pseudo second-order kinetics modified to include a term for inactivation/death of cells.
Boonchayaanant
Benjaporn
B
Department of Civil and Environmental Engineering, Stanford University, 380 Panama Mall, Terman Building M11, Stanford, California 94305-4020, USA.
Kitanidis
Peter K
PK
Criddle
Craig S
CS
D003901:Desulfovibrio / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D010084:Oxidation-Reduction; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017423:Sequence Analysis, RNA; D013431:Sulfates / Q000378:metabolism*; D013696:Temperature; D014501:Uranium / Q000378:metabolism*
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D000431:Ethanol; D014501:Uranium