title: The radiosensitizing effect of doranidazole on human colorectal cancer cells exposed to high doses of irradiation.
abstract: BACKGROUND
  This paper investigates the effects of a new radiosensitizer, doranidazole, and enhancing irradiation on colorectal cancer cells.
  
  
  METHODS
  The radiosensitizing effect of doranidazole was determined using colony formation and propidium iodide (PI) assays to measure cell growth inhibition and the cell killing effect of human colorectal cancer cell lines exposed to high doses of gamma-ray irradiation under hypoxic conditions in vitro. Fluorescence staining and cell migration assays were also used to assess the radiosensitizing effect.
  
  
  RESULTS
  Cell proliferation evaluated by clonogenic survival curves was significantly inhibited by 5 mmol/L doranidazole, particularly at doses ranging from 10 to 30 Gy of irradiation. The radiosensitizing effect of doranidazole on colorectal cancer cells occurs in a time- and dose-dependent manner. Doranidazole also inhibited the mobility of cell invasion and migration.
  
  
  CONCLUSIONS
  Doranidazole can enhance the killing effect and the cell growth inhibition of colorectal cancer after high-dose irradiation in a time and dose-dependent manner.
authors.affiliation: Department of Central Laboratory, Dalian Municipal Central Hospital, Xinan-Road 826, Dalian, 116033, China. tyouri19652004@yahoo.com.cn
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Aimin
authors.initials: A
authors.lastname: Gong
authors.forename: Jun
authors.initials: J
authors.lastname: Ji
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wu
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhu
authors.forename: Suqing
authors.initials: S
authors.lastname: Lv
authors.forename: Hongzhu
authors.initials: H
authors.lastname: Lv
authors.forename: Xizhuo
authors.initials: X
authors.lastname: Sun
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000532:radiotherapy*; D005720:Gamma Rays / Q000627:therapeutic use; D006801:Humans; D007093:Imidazoles / Q000627:therapeutic use*; D011838:Radiation-Sensitizing Agents / Q000627:therapeutic use*
chemical_list: D007093:Imidazoles; D011838:Radiation-Sensitizing Agents; C106738:doranidazole