[Expression of augmenter of liver regeneration in cryptorchidism spermatogenic cells and its implication].
OBJECTIVE
To investigate the expression of the augmenter of liver regeneration (ALR) in cryptorchidism spermatogenic cells.


METHODS
Twenty cryptorchidism rat models were established by surgery, 10 included as normal controls and anther 10 as sham-surgery controls. The expression of ALR was detected by immunochemistry, the COX II level measured by immunofluorescence and the ferric iron contents assayed by Perls stain.


RESULTS
ALR expressed intensively in the spermatogonia of the control groups but in a signigicantly diminished manner in the cryptorchidism group. No significant difference was found in the COX II level between any two groups of the same age. Ferric iron content of the PND30 cryptorchids decreased significantly (P < 0.05).


CONCLUSIONS
ALR may play an important role in early spermatogenesis. Metabolism dysfunctions caused by ALR defection might be a crucial mechanism for aspermatogenesis of cryptorchidism.
Chen
Xuan
X
Department of Anesthesiology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, China. novembermarie@163.com
Zhang
De-ying
DY
Liu
Xing
X
Wei
Guang-hui
GH
D000818:Animals; D003456:Cryptorchidism / Q000378:metabolism / Q000473:pathology*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004195:Disease Models, Animal; D005455:Fluorescent Antibody Technique; D005854:Germ Cells / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D050862:Oxidoreductases Acting on Sulfur Group Donors
D051546:Cyclooxygenase 2; D011506:Proteins; C088825:GFER protein, rat; D050862:Oxidoreductases Acting on Sulfur Group Donors