Development of a new immunosensor for the detection of dopamine.
Graphite immunoelectrodes as immunosensors using indirect immobilization of a hapten were investigated for their applicability to detect dopamine hydrochloride at low levels. Conditions were optimized to achieve the highest sensitivity using the indirect immobilization of dopamine hydrochloride through a polymerized glutaraldehyde network on microtiter plates using ELISA technique. The conditions were later transferred to the graphite rods (phi 0.8 m x 20 mm) and a comparison between the two different sensitivities (IC50 midpoint of test) was carried out. Graphite electrodes showed higher sensitivity towards dopamine than ELISA, since they were able to detect dopamine with a midpoint of test of 0.2 mmol/l while using ELISA they were able to detect dopamine hydrochloride at 2 mmol/l.
Fouad
Dina
D
Chemistry Department, Faculty of Science, Assiut University, Assiut 71516, Egypt. dinafouad93@hotmail.com
D002138:Calibration; D004298:Dopamine / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006108:Graphite; D007118:Immunoassay / Q000379:methods; D012680:Sensitivity and Specificity
D006108:Graphite; D004298:Dopamine