title: A novel acidophile community populating waste ore deposits at an acid mine drainage site.
abstract: Waste ore samples (pH 3.0) were collected at an acid mine drainage (AMD) site in Anhui, China. The present acidophilic microbial community in the waste ore was studied with 16S rRNA gene clone library and denaturing gradient gel electrophoresis (DGGE). Eighteen different clones were identified and affiliated with Actinobacteria, low G + C Gram-positives, Thermomicrobia, Acidobacteria, Proteobacteria, candidate division TM7, and Planctomycetes. Phylogenetic analysis of 16S rRNA gene sequences revealed a diversity of acidophiles in the samples that were mostly novel. It is unexpected that the moderately thermophilic acidophiles were abundant in the acidic ecosystem and may play a great role in the generation of AMD. The result of DGGE was consistent with that of clone library analysis. These findings help in the better understanding of the generation mechanism of AMD and in developing a more efficient method to control AMD.
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. hao20709@yahoo.com.cn
authors.forename: Chun-bo
authors.initials: CB
authors.lastname: Hao
authors.forename: Hong-xun
authors.initials: HX
authors.lastname: Zhang
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Bai
authors.forename: Qing
authors.initials: Q
authors.lastname: Hu
authors.forename: Bao-guo
authors.initials: BG
authors.lastname: Zhang
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004783:Environmental Microbiology; D007220:Industrial Waste; D008906:Mining; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D007220:Industrial Waste; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S