Quantitative expression analysis in peripheral blood of patients with chronic myeloid leukaemia: correlation between HMGA2 expression and white blood cell count.
The architectural transcription factor HMGA2 is highly expressed during embryogenesis but scarcely detectable in non-dividing adult cells. Previously, HMGA2 re-expression was detected in blood from CML patients by conventional RT-PCR, while blood samples from healthy volunteers were HMGA2 negative. Using the sensitive method of real-time quantitative RT-PCR, herein HMGA2 expression was detectable not only in peripheral blood from leukaemia patients but also in blood from healthy donors. Statistical analysis revealed a highly significant correlation between white blood cell count and HMGA2 transcript levels. The results indicate that up-regulation of HMGA2 expression is correlated to the undifferentiated phenotype of leukaemic cells accumulating during progression of chronic phase to blast crisis.
Meyer
B
B
Centre for Human Genetics, University of Bremen, Bremen, Germany.
Krisponeit
D
D
Junghanss
C
C
Murua Escobar
H
H
Bullerdiek
J
J
D000328:Adult; D000368:Aged; D001752:Blast Crisis; D018450:Disease Progression; D015973:Gene Expression Regulation, Leukemic; D025743:HMGA2 Protein / Q000096:biosynthesis*; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000378:metabolism; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood* / Q000378:metabolism*; D007958:Leukocyte Count; D008875:Middle Aged; D015233:Models, Statistical; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016896:Treatment Outcome; D015854:Up-Regulation
D025743:HMGA2 Protein