Involvement of GABA(A) receptors in the neuroprotective effect of theanine on focal cerebral ischemia in mice.
We investigated the involvement of gamma-aminobutyric acid(A) (GABA(A)) receptors in the neuroprotective effect of gamma-glutamylethylamide (theanine), a component of Japanese green tea, following a 4-h middle cerebral artery (MCA) occlusion in mice. Theanine (1 mg/kg) reduced the size of the cerebral infarct and alterations of NeuN, GFAP, and Iba 1 expression levels at 24 h after MCA occlusion. This neuroprotective effect of theanine was prevented by bicuculline (GABA(A)-receptor antagonist, 10 mg/kg) but not 3-mercaptopropionic acid (glutamate decarboxylase inhibitor). These results suggest that the neuroprotective effect of theanine is mediated, at least in part, by GABA(A) receptors.
Egashira
Nobuaki
N
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
Hayakawa
Kazuhide
K
Osajima
Megumi
M
Mishima
Kenichi
K
Iwasaki
Katsunori
K
Oishi
Ryozo
R
Fujiwara
Michihiro
M
D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy*; D002135:Calcium-Binding Proteins / Q000187:drug effects / Q000378:metabolism; D028241:Camellia sinensis / Q000737:chemistry; D004268:DNA-Binding Proteins; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000187:drug effects / Q000378:metabolism; D005971:Glutamates / Q000494:pharmacology*; D020244:Infarction, Middle Cerebral Artery; D008297:Male; D051379:Mice; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009687:Nuclear Proteins / Q000187:drug effects / Q000378:metabolism; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000378:metabolism; D013662:Tea / Q000737:chemistry
C491049:Aif1 protein, mouse; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D005971:Glutamates; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; C492380:NeuN protein, mouse; D018696:Neuroprotective Agents; D009687:Nuclear Proteins; D011963:Receptors, GABA-A; D013662:Tea; C026166:theanine