[Creation of tissue engineered cartilage with internal support].
OBJECTIVE
To test the hypothesis that tissue-engineered cartilage can be bioincorporated with a nonreactive, permanent endoskeletal scaffold.


METHODS
Chondrocytes obtained from swine articular were seeded onto polyglycolic acids(PGA) scaffold which was incorporated with high-density polyethylene (Medpor). After cultured in vitro for two weeks,the cell-scaffold construct was implanted into subcutaneous pockets on the back of nude mice. Six weeks later,the newly formed cartilage prosthesis was harvested, and a small part of sample was evaluated by gross view, histology, type II collagen immunohistochemistry and biochemistry. PGA scaffold seeded with cells as the control group.


RESULTS
The newly formed cartilage was very similar to normal cartilage in both gross view and histology, and jointed Medpor tightly. The center of control group was hollow.


CONCLUSIONS
This pilot technique combining tissue engineering with a permanent success in creating cartilage without "hollow" phenomenon. biocompatible endoskeleton demonstrated
Wu
Yu-jia
YJ
Department of Plastic Surgery, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China.
Jiang
Hua
H
Zhou
Guang-dong
GD
Xia
Wan-yao
WY
Luo
Xu-song
XS
Liu
Xia
X
D000818:Animals; D001672:Biocompatible Materials; D002356:Cartilage / Q000637:transplantation*; D019902:Chondrocytes / Q000166:cytology; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010865:Pilot Projects; D011095:Polyethylenes; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; C084562:Medpor; D011095:Polyethylenes