Surfactin C inhibits Mycoplasma hyopneumoniae-induced transcription of proinflammatory cytokines and nitric oxide production in murine RAW 264.7 cells.
Intact pathogenic Mycoplasma hyopneumoniae at 100 microg protein ml(-1) induced transcription of proinflammatory cytokines such as cyclooxygenase (COX)-2, tumor necrosis factor (TNF)-alpha, interleukin(IL)-1, IL-6 and inducible nitric oxide synthase (iNOS) in RAW 264.7 cells. After pretreatment with 50 microg surfactin C/ml, purified from Bacillus subtilis, transcription of the COX-2, IL-1beta, IL-6 and iNOS genes induced by M. hyopneumoniae was inhibited by 43%, 82%, 72% and 59%, respectively.
Hwang
Mi-Hyun
MH
College of Veterinary Medicine, Kyungpook National University, 702-701 Daegu, Republic of Korea.
Chang
Zhi-Qiang
ZQ
Kang
Eun-Hee
EH
Lim
Jong-Hwan
JH
Yun
Hyo-In
HI
Rhee
Man-Hee
MH
Jeong
Kyu-Shik
KS
Park
Seung-Chun
SC
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects; D007249:Inflammation / Q000276:immunology*; D055666:Lipopeptides; D051379:Mice; D045705:Mycoplasma hyopneumoniae / Q000276:immunology*; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000235:genetics; D010456:Peptides, Cyclic / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D055666:Lipopeptides; D010456:Peptides, Cyclic; D012333:RNA, Messenger; C009365:surfactin peptide; D009569:Nitric Oxide