title: Identification and monitoring of effector and regulatory T cells during experimental arthritis based on differential expression of CD25 and CD134.
abstract: Major problems in the analysis of CD4+ effector cell and regulatory T cell (Treg) populations in an activated immune system are caused by the facts that both cell types can express CD25 and that the discriminatory marker forkhead box p3 can only be analyzed in nonviable (permeabilized) cells. Here, we show that CD134 (OX40) can be used as a discriminatory marker combined with CD25 to isolate and characterize viable CD4+ effector cells and Tregs. Before and during adjuvant arthritis in rats, coexpression of CD134 and CD25 identified activated Tregs consistently, as these T cells proliferated poorly to disease-associated antigens and were suppressive in vitro and in vivo. Depending on the time of isolation and location, CD4+ T cell populations expressing CD134 or CD25 contained effector/memory T cells. Analysis of the function, phenotype, and amount of the CD4+ T cell subsets in different lymph node stations revealed spatiotemporal differences in effector cell and Treg compartments during experimental arthritis.
authors.affiliation: Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 2, 3584CM Utrecht, The Netherlands.
authors.forename: Esther N M
authors.initials: EN
authors.lastname: Nolte-'t Hoen
authors.forename: Elmieke P J
authors.initials: EP
authors.lastname: Boot
authors.forename: Josée P A
authors.initials: JP
authors.lastname: Wagenaar-Hilbers
authors.forename: Jolanda H M
authors.initials: JH
authors.lastname: van Bilsen
authors.forename: Ger J A
authors.initials: GJ
authors.lastname: Arkesteijn
authors.forename: Gert
authors.initials: G
authors.lastname: Storm
authors.forename: Linda A
authors.initials: LA
authors.lastname: Everse
authors.forename: Willem
authors.initials: W
authors.lastname: van Eden
authors.forename: Marca H M
authors.initials: MH
authors.lastname: Wauben
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology*; D004195:Disease Models, Animal; D018450:Disease Progression; D051858:Forkhead Transcription Factors / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis* / Q000276:immunology; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D053262:Receptors, OX40 / Q000096:biosynthesis* / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D051858:Forkhead Transcription Factors; C500522:Foxp3 protein, rat; D053645:Interleukin-2 Receptor alpha Subunit; D053262:Receptors, OX40