Preparation and blood coagulation evaluation of chitosan microspheres.
Cross-linked chitosan microspheres (40-100 microm) with smooth surface were prepared by the methods of emulsification and ethanol coagulant. FTIR results showed that the cross-linking reaction occurred on the amino groups of chitosan molecules. The swelling characteristic of chitosan microspheres was influenced by the environment pH, being generally greater at low rather than higher pH values. The coagulation properties of chitosan microspheres were evaluated by dynamic blood clotting, platelet adhesion and activation, erythrocyte adhesion, hemolysis, and protein absorption assays. Chitosan microspheres can shorten the clotting time and induce the adhesion and activation of platelets. But the shortening of clotting time by chitosan microspheres may be related to not only platelet aggregation, but also erythrocyte aggregation. Take together, chitosan microspheres may be potential use as thrombospheres.
Wang
Qi Zhao
QZ
College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao 266003, P.R. China.
Chen
Xi Guang
XG
Li
Zi Xiang
ZX
Wang
Song
S
Liu
Cheng Sheng
CS
Meng
Xiang Hong
XH
Liu
Chen Guang
CG
Lv
Ying Hui
YH
Yu
Le Jun
le J
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D002417:Cattle; D048271:Chitosan / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004903:Erythrocyte Aggregation / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006490:Hemostatics / Q000138:chemical synthesis / Q000494:pharmacology; D066298:In Vitro Techniques; D008863:Microspheres; D010316:Particle Size; D010973:Platelet Adhesiveness / Q000187:drug effects; D011817:Rabbits
D006490:Hemostatics; D048271:Chitosan