title: In vitro analysis of His-Asp phosphorelays in Aspergillus nidulans: the first direct biochemical evidence for the existence of His-Asp phosphotransfer systems in filamentous fungi.
abstract: His-Asp phosphorelays are widespread signal transduction mechanisms in bacteria, fungi, and higher plants. In order to investigate a His-Asp phosphorelay network in filamentous fungi, which has been genetically characterized in part, we attempted to construct an in vitro phosphotransfer network in Aspergillus nidulans comprising all the necessary components. As a first step, we established an in vitro phosphotransfer system with a histidine-containing phosphotransmitter YpdA, a response regulator SrrA, and a bacterial histidine kinase ArcB as a phosphate donor. We demonstrated the phosphotransfer from ArcB to A. nidulans YpdA and the subsequent transfer from YpdA to SrrA. This is the first direct biochemical evidence for the presence of the phosphotransfer system in filamentous fungi. Furthermore, a retrograde phosphorylation from YpdA to FphA, a histidine kinase similar to bacterial phytochrome, was found. The overall picture of the His-Asp phosphorelays in A. nidulans is discussed based on the results of the in vitro study.
authors.affiliation: Laboratory of Gene Regulation, Graduate School of Bioagricultural Sciences, Nagoya University, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Azuma
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kanamaru
authors.forename: Akinori
authors.initials: A
authors.lastname: Matsushika
authors.forename: Takafumi
authors.initials: T
authors.lastname: Yamashino
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mizuno
authors.forename: Masashi
authors.initials: M
authors.lastname: Kato
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kobayashi
mesh_terms: D001224:Aspartic Acid / Q000378:metabolism*; D001233:Aspergillus nidulans / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006639:Histidine / Q000378:metabolism*; D000071677:Histidine Kinase; D066298:In Vitro Techniques; D008954:Models, Biological; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D001224:Aspartic Acid; D006639:Histidine; D011494:Protein Kinases; D000071677:Histidine Kinase