Boronic acid-lectin affinity chromatography. 1. Simultaneous glycoprotein binding with selective or combined elution.
We introduce a novel combination of boronic acid affinity chromatography with lectin affinity chromatography, dubbed as boronic acid-lectin affinity chromatography (BLAC). Concanavalin A and wheat germ agglutinin lectins were mixed with the pesudo-lectin boronic acid to form the BLAC affinity column and their performance was evaluated with standard glycoproteins. Optimization of the binding and elution buffers for the BLAC system is described. The BLAC columns were employed to isolate glycoproteins of interest using both selective and/or combined elution.
Monzo
Alex
A
Horváth Laboratory of Bioseparation Sciences, Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck, Innrain 66, 6020, Innsbruck, Austria.
Bonn
Guenther K
GK
Guttman
András
A
D001897:Boronic Acids / Q000737:chemistry*; D002021:Buffers; D002846:Chromatography, Affinity / Q000379:methods*; D003208:Concanavalin A / Q000737:chemistry; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D037102:Lectins / Q000737:chemistry*; D011485:Protein Binding; D014909:Wheat Germ Agglutinins / Q000737:chemistry
D001897:Boronic Acids; D002021:Buffers; D006023:Glycoproteins; D037102:Lectins; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A