Impact of Zn, Cu, and Fe on the activity of carbonic anhydrase of erythrocytes in ducks.
The impact of zinc, copper, and iron on the duck erythrocyte carbonic anhydrase (CA) activity and the hemoglobin content in vitro culture were studied. The increase of zinc or iron addition at a low level induced the rise of CA activity, and the CA activity was inhibited by zinc or iron at a high addition level. The duck erythrocyte CA was strongly inhibited by cupric ion. The inhibition constant of duck erythrocyte CA to cupric ion is about 3.5 microM. Carbonic anhydrase compared to hemoglobin is more sensitive to zinc and copper in the environment. These findings suggest that some characteristics of duck erythrocyte CA are different from both CAI and CAII of mammals. The increase of Fe addition below 8 microM in the minimal essential medium brought about the rise of CA activity and resulted in the maximum of CA activity exceeding that induced by Zn. It provided a new evidence for the role of ferrous ion in CA.
Wu
Yanyou
Y
Jiangsu Provincial Key Laboratory of Modern Agricultural Equipment and Technology, Institute of Agricultural Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 210213, People's Republic of China. yanyouwu@ujs.edu.cn
Zhao
Xinzhen
X
Li
Pingping
P
Huang
Huakun
H
D000818:Animals; D002256:Carbonic Anhydrases / Q000032:analysis* / Q000097:blood* / Q000737:chemistry; D002470:Cell Survival; D003300:Copper / Q000032:analysis*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D004372:Ducks; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism; D007501:Iron / Q000032:analysis*; D013997:Time Factors; D014131:Trace Elements / Q000737:chemistry*; D015032:Zinc / Q000032:analysis* / Q000494:pharmacology
D006454:Hemoglobins; D014131:Trace Elements; D003300:Copper; D007501:Iron; D002256:Carbonic Anhydrases; D015032:Zinc