Sequence analysis of Vietnamese P[6] rotavirus strains suggests evidence of interspecies transmission.
Nucleotide and amino acid sequences of the VP8* gene of five Vietnamese P[6] rotavirus strains detected from hospitalized patients with acute gastroenteritis were analyzed and compared with other human and porcine P[6] rotaviruses. It is of interest that these strains had greatest identity with two Italian porcine rotavirus strains, 134/04-10 and 134/04-11. To our knowledge, these five Vietnamese rotaviruses are the rare P[6] rotavirus strains belonging to lineage I that cluster into sublineage Ic with porcine rotaviruses, and not into sublineage Ia, as other human P[6] rotaviruses have done so far. Sequence analysis of the VP7 gene of these P[6] rotavirus strains was also performed. The results showed that the Vietnamese G9P[6] strain had high similarity with other human G9 rotaviruses, confirming a human-animal reassortant virus, whereas other three G4P[6] strains had best identity with porcine G4 rotavirus strains, suggesting interspecies transmission of rotavirus between porcine and humans. This result provides the important data on molecular characteristics of Vietnamese rotaviruses, and highlights interspecies transmission events of rotaviruses in Vietnam as well as in Asia.
Nguyen
Tuan Anh
TA
Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Khamrin
Pattara
P
Trinh
Quang Duy
QD
Phan
Tung Gia
TG
Pham
Le Duc
le D
Hoang
Le Phuc
le P
Hoang
Kim Trong
KT
Yagyu
Fumihiro
F
Okitsu
Shoko
S
Ushijima
Hiroshi
H
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D006801:Humans; D010802:Phylogeny; D016601:RNA-Binding Proteins / Q000235:genetics; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000502:physiology; D012400:Rotavirus Infections / Q000453:epidemiology / Q000635:transmission* / Q000821:virology*; D013552:Swine; D014744:Vietnam / Q000453:epidemiology; D017361:Viral Nonstructural Proteins / Q000235:genetics
D000956:Antigens, Viral; D036022:Capsid Proteins; D016601:RNA-Binding Proteins; C050779:VP7 protein, Rotavirus; D017361:Viral Nonstructural Proteins; C079234:NS35 protein, rotavirus