title: New transposon delivery plasmids for insertional mutagenesis in Bacillus anthracis.
abstract: Two new transposon delivery vector systems utilizing Mariner and mini-Tn10 transposons have been developed for in vivo insertional mutagenesis in Bacillus anthracis and other compatible Gram-positive species. The utility of both systems was directly demonstrated through the mutagenesis of a widely used B. anthracis strain.
authors.affiliation: The Scripps Research Institute, Department of Molecular and Experimental, Medicine, Division of Cellular Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, United States.
authors.forename: Adam C
authors.initials: AC
authors.lastname: Wilson
authors.forename: Marta
authors.initials: M
authors.lastname: Perego
authors.forename: James A
authors.initials: JA
authors.lastname: Hoch
mesh_terms: D001408:Bacillus anthracis / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D018014:Gene Transfer Techniques; D005798:Genes, Bacterial / Q000502:physiology; D005822:Genetic Vectors; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements