title: Detection of M. tuberculosis in clinical samples of diversified nature by IS6110 based PCR.
abstract: Performance of the polymerase chain reaction technique based on IS6110 sequence was evaluated in clinical samples obtained from pulmonary and extrapulmonary cases of tuberculosis. One hundred and seventy two samples were processed for detection of M. tuberculosis by ZN stained smear examination, LJ medium culture, BACTEC radiometric culture and PCR tests amplifying 123bp region of IS6110 sequence. A significant difference was seen in the sensitivities of different tests, the figures being 83% for PCR test, 35.2% for smear examination, 47.16% for LJ culture and 53.45% for BACTEC culture (p < 0.05). However, no significant difference was found as far as specificity was concerned. PCR test sensitivity in. pulmonary and extrapulmonary clinical samples were 90.14% and 77.27% respectively and found to be significantly higher (p < 0.05) when compared with those of other tests. The mean detection time for M. tuberculosis was 24.03 days by LJ medium culture, 12.89 days by BACTEC culture and less than one day by PCR test. PCR based on IS6100 sequence is highly sensitive method for the early diagnosis of pulmonary and extrapulmonary tuberculosis.
authors.affiliation: Depart of Microbiology, Maulana Azad Medical College and Associated Lok Nayak Hospital, New Delhi, India.
authors.forename: Sanjay Singh
authors.initials: SS
authors.lastname: Negi
authors.forename: Ranjana
authors.initials: R
authors.lastname: Anand
authors.forename: Syed Tazeen
authors.initials: ST
authors.lastname: Pasha
authors.forename: Seemi Farhat
authors.initials: SF
authors.lastname: Basir
authors.forename: Sunil
authors.initials: S
authors.lastname: Gupta
authors.forename: Shashi
authors.initials: S
authors.lastname: Khare
authors.forename: Shiv
authors.initials: S
authors.lastname: Lal
mesh_terms: D001202:Ascitic Fluid / Q000382:microbiology; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012661:Semen / Q000382:microbiology; D012867:Skin / Q000382:microbiology; D013183:Sputum / Q000382:microbiology; D013492:Suppuration / Q000382:microbiology; D013582:Synovial Fluid / Q000382:microbiology; D014376:Tuberculosis / Q000097:blood / Q000134:cerebrospinal fluid / Q000382:microbiology* / Q000652:urine