title: Studies on the antioxidant activity of the essential oil and methanol extract of Marrubium globosum subsp. globosum (lamiaceae) by three different chemical assays.
abstract: This study is designed to examine the chemical composition and in vitro antioxidant activity of the essential oil and sub-fractions of the methanol extract of Marrubium globosum subsp. globosum. The GC and GC-MS analysis of the essential oil were resulted in the determination of 84 components representing 88.2% of the oil. The major constituents of the oil were spathulenol (15.8%), beta-caryophyllene (9.0%), caryophyllene oxide (7.9%), germacrene D (6.5%), and bicyclogermacrene (3.1%). Antioxidant activities of the samples were determined by three different test systems namely DPPH, beta-carotene/linoleic acid and reducing power assay. In DPPH system, the weakest radical scavenging activity was exhibited by the essential oil (1203.38+/-7.18 microg ml(-1)). Antioxidant activity of the polar sub-fraction of methanol extract was superior to the all samples tested with an EC(50) value of 157.26+/-1.12 microg ml(-1). In the second case, the inhibition capacity (%) of the polar sub-fraction of methanol extract (97.39%+/-0.84) was found the strongest one, which is almost equal to the inhibition capacity of positive control BHT (97.44%+/-0.74). In the case of reducing power assay, a similar activity pattern was observed as given in the first two systems. Polar sub-fraction was the strongest radical reducer when compared with the non-polar one, with an EC(50) value of 625.63+/-1.02 microg ml(-1). The amount of the total phenolics was highest in polar sub-fraction (25.60+/-0.74 microg/mg). A positive correlation was observed between the antioxidant activity potential and total phenolic level of the extracts. On the other hand, total flavonoid content was found equal for the both sub-fractions.
authors.affiliation: Faculty of Science and Literature, Department of Chemistry, Mugla University, Mugla 48000, Turkey.
authors.forename: Cengiz
authors.initials: C
authors.lastname: Sarikurkcu
authors.forename: Bektas
authors.initials: B
authors.lastname: Tepe
authors.forename: Dimitra
authors.initials: D
authors.lastname: Daferera
authors.forename: Moschos
authors.initials: M
authors.lastname: Polissiou
authors.forename: Mansur
authors.initials: M
authors.lastname: Harmandar
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods*; D001713:Biphenyl Compounds / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods; D005419:Flavonoids / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006834:Hydrazines / Q000737:chemistry; D019686:Lamiaceae / Q000378:metabolism; D019787:Linoleic Acid / Q000737:chemistry; D027522:Marrubium / Q000378:metabolism*; D000432:Methanol / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry*; D019800:Phenol / Q000737:chemistry; D010851:Picrates; D010936:Plant Extracts / Q000032:analysis*; D019207:beta Carotene / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D006834:Hydrazines; D009822:Oils, Volatile; D010851:Picrates; D010936:Plant Extracts; D019207:beta Carotene; D019800:Phenol; D019787:Linoleic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol