[Empirical study of the transportation of pulmonary surfactant-super oxide dismutase liposome to lung tissue in rats].
OBJECTIVE
To study the feasibility of the transportation of pulmonary surfactant-super oxide dismutase (PS-SOD) liposome to lung tissue in rats.


METHODS
32 Wistar rats were randomly divided into 4 groups (8 rats in each group): normal saline group, PS group, SOD group, PS-SOD liposome group. Each group was further divided into two groups (4 rats in each group), and the rats were respectively killed 2 and 24 hours after the operation. While the biological activity of SOD in irrigating solution and tissue homogenate were detected, lung tissue were labeled with fluorescent and then observed under microscope and transmission electron microscope.


RESULTS
PS-SOD liposome was corps rounds with monolayer lipid with stable surface tension and antioxidative activity. At the point of 2 hours after operation, while the SOD biological activity of irrigating solution in PS-SOD liposome group (32.87 +/- 5.47) and SOD group (33.14 +/- 5.61) were obviously higher than that in normal saline group (2.15 +/- 0.17, P < 0.01), there was no difference between them (P > 0.05). The mean fluorescence optical density in PS-SOD liposome group (0.109 +/- 0.018) was lower than that in normal saline group (0.144 +/- 0.052) and PS group (0.143 +/- 0.026, P < 0.01). 24 hours after operation, the SOD biological activity of irrigating solution in PS-SOD liposome group (11.54 +/- 1.42) was the highest (P < 0.01) and the mean fluorescence optical density in PS-SOD liposome group (0.112 +/- 0.018) was the lowest (P < 0.01). The SOD biological activity of tissue homogenate in PS-SOD liposome group (2 h: 16.83 +/- 2.69, 24 h: 15.70 +/- 2.75) was higher than that in normal saline group (2 h: 5.79 +/- 0.93, 24 h: 5.84 +/- 1.31) and in SOD group (2 h: 7.07 +/- 1.04, 24 h: 6.11 +/- 1.06, P < 0.01) both at the point of 2 and 24 hours after the operation. Lots of PS-SOD liposome was observed in type II alveolar epithelial cells under transmission electron microscope.


CONCLUSIONS
Intrathecal administ ration of PS-SOD liposome enhanced the transportation of SOD into lung tissue and its antioxidative activity.
Xing
Quan-sheng
QS
Qingdao Children's Heart Center, Qingdao 266011, China.
Wu
Qin
Q
Pan
Yu-zhu
YZ
Cao
Qian
Q
Rong
You-bao
YB
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016503:Drug Delivery Systems; D005240:Feasibility Studies; D008081:Liposomes / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D008168:Lung / Q000378:metabolism* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D011663:Pulmonary Surfactants / Q000737:chemistry / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism*
D008081:Liposomes; D011663:Pulmonary Surfactants; D013482:Superoxide Dismutase