Fatty acids in component of milk enhance the expression of the cAMP-response-element-binding-protein-binding protein (CBP)/p300 gene in developing rats.
Fatty acids in milk are thought to play an important role in intestinal maturation and gene expression in the rat small intestine during the suckling-weaning period. In the present study, we determined the jejunal mRNA level of the cAMP-response-element-binding-protein-binding protein (CBP)/p300, which is one of the chromatin remodelling factors and regulates histone acetylation, during the postnatal period in rats. The mRNA level of CBP/p300 was high during the suckling and middle of the weaning period (day 5 to 20) and then declined sharply to a low level at the end of the weaning period and after weaning. In situ hybridisation also showed that CBP/p300 mRNA levels in the villus as well as the basal membrane clearly decreased after weaning. Rat pups at age 17 d, weaned to a high-fat diet, showed higher levels of CBP/p300 mRNA than those weaned to a low-fat diet. Oral administration of caprylic acid, oleic acid and linoleic acid, which are major fatty acid components in milk, induced jejunal CBP/p300 gene expression. The present results suggest that fatty acids in components of milk enhance expression of the CBP/p300 genes in the small intestine.
Mochizuki
Kazuki
K
School of Food and Nutritional Sciences, The University of Shizuoka, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526, Japan.
Kawai
Hiromi
H
Mochizuki
Hiroko
H
Shimada
Masaya
M
Takase
Sachiko
S
Goda
Toshinao
T
D000818:Animals; D017362:Cyclic AMP Response Element-Binding Protein / Q000096:biosynthesis* / Q000235:genetics; D004041:Dietary Fats / Q000494:pharmacology*; D050881:E1A-Associated p300 Protein / Q000096:biosynthesis* / Q000235:genetics; D005227:Fatty Acids / Q000494:pharmacology*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D017403:In Situ Hybridization / Q000379:methods; D007583:Jejunum / Q000254:growth & development / Q000378:metabolism; D007774:Lactation; D008892:Milk / Q000737:chemistry*; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014886:Weaning
D017362:Cyclic AMP Response Element-Binding Protein; D004041:Dietary Fats; D005227:Fatty Acids; D012333:RNA, Messenger; D050881:E1A-Associated p300 Protein; C494362:Ep300 protein, rat