A retrospective study and gene analysis of canine sterile panniculitis.
In this study, a retrospective analysis was conducted to assess the current aspects and predisposing factors of canine sterile panniculitis. Miniature dachshund, neutered, and younger dogs appeared to be predisposed. In addition, histories of previous surgery and injection were associated in 46.5% of the cases, with several types of surgical suture materials used. About 88% of the dogs had multifocal lesions, frequently with signs of systemic illnesses. Whereas systemic immunosuppressive therapy was effective in most dogs, surgical excision of lesions was rarely curative. In order to prevent recurrences, over 65% of the cases required prolonged immunosuppressive therapy. Polymorphism of canine alpha(1)AT gene was investigated as a candidate gene for sterile panniculitis. Eight polymorphisms were discovered in seven Miniature dachshunds by direct nucleotide sequencing, which included a 12-bp deletion, three non-synonymous single nucleotide polymorphisms, and four silent substitutions. Genotyping of the two polymorphisms, c.109_120del12 and c.483A>C, which identified at high incidence in the dachshunds, was conducted in 83 dogs of 6 popular breeds. The frequencies of neither polymorphism differed between Miniature dachshunds and other breeds, suggesting that neither is responsible for developing panniculitis.
Yamagishi
Chie
C
Department of Veterinary Internal Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
Momoi
Yasuyuki
Y
Kobayashi
Tetsuro
T
Ide
Kaori
K
Ohno
Koichi
K
Tsujimoto
Hajime
H
Iwasaki
Toshiroh
T
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004283:Dog Diseases / Q000209:etiology* / Q000235:genetics; D004285:Dogs; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D008297:Male; D008969:Molecular Sequence Data; D015434:Panniculitis / Q000209:etiology* / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017385:Sequence Homology; D000515:alpha 1-Antitrypsin / Q000235:genetics*
D000515:alpha 1-Antitrypsin