High-level expression of recombinant active human renin in Sf-9 cells: rapid purification and characterization.
Recombinant human (rh) renin was expressed in Sf-9 insect cells. Baculovirus-infected Sf-9 cells produced active rh-renin in the late stage of cultivation. The rh-renin was purified after 5 d of culture by two steps of column chromatography. Approximately 0.61 mg of pure rh-renin was obtained from 200 ml of culture medium with a yield of 35.3%.
Takahashi
Saori
S
Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Center, Japan. saori@arif.pref.akita.jp
Hata
Keishi
K
Kikuchi
Ken-Ichi
K
Gotoh
Takeshi
T
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D012083:Renin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018411:Spodoptera / Q000166:cytology* / Q000378:metabolism*; D013997:Time Factors; D014343:Trypan Blue / Q000494:pharmacology
D004396:Coloring Agents; D011994:Recombinant Proteins; D012083:Renin; D014343:Trypan Blue