The spectrum of Trp- mutants isolated as 5-fluoroanthranilate-resistant clones in Saccharomyces bayanus, S. mikatae and S. paradoxus.
5-Fluoroanthranilic acid (FAA)-resistant mutants were selected in homothallic diploids of three Saccharomyces species, taking care to isolate mutants of independent origin. Mutations were assigned to complementation groups by interspecific complementation with S. cerevisiae tester strains. In all three species, trp3, trp4 and trp5 mutants were recovered. trp1 mutants were also recovered if the selection was imposed on a haploid strain. Thus, FAA selection may be more generally applicable than was previously described.
Jones
Elizabeth W
EW
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA. ej09@andrew.cmu.edu
Berget
Peter B
PB
Burnette
James M
JM
Anderson
Candice
C
Asafu-Adjei
Denise
D
Avetisian
Seda
S
Barrie
Fatmata
F
Chen
Ruby
R
Chu
Bur
B
Conroy
Samantha
S
Conroy
Sean
S
Dill
Allyson
A
Eimer
Will
W
Garrity
Diane
D
Greenwood
Alexander
A
Hamilton
Tamara
T
Hucko
Simon
S
Jackson
Carmen
C
Livesey
Kristen
K
Monaco
Tiffany
T
Onorato
Christina
C
Otsuka
Mai
M
Pai
Satyan
S
Schaeffer
George
G
Shung
Sharon
S
Spath
Samantha
S
Stahlman
Jonathan
J
Sweeney
Blake
B
Wiltrout
Elizabeth
E
Yurovsky
Daniel
D
Zonneveld
Andrea
A
D019747:Aldose-Ketose Isomerases / Q000235:genetics / Q000378:metabolism; D000878:Anthranilate Synthase / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics; D005816:Genetic Complementation Test; D007209:Indole-3-Glycerol-Phosphate Synthase / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D012440:Saccharomyces / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014364:Tryptophan / Q000235:genetics* / Q000378:metabolism; D062367:ortho-Aminobenzoates / Q000378:metabolism / Q000494:pharmacology*
D005656:Fungal Proteins; D062367:ortho-Aminobenzoates; D014364:Tryptophan; D007209:Indole-3-Glycerol-Phosphate Synthase; D000878:Anthranilate Synthase; D019747:Aldose-Ketose Isomerases