title: Effect of arsenic and chromium on the serum amino-transferases activity in Indian major carp, Labeo rohita.
abstract: Arsenic and hexavalent chromium toxicity results from their ability to interact with sulfahydryl groups of proteins and enzymes, and to substitute phosphorus in a variety of biochemical reactions. Alanine aminotransferase (ALT; E.C: 2.6.1.2) and Aspartate amino transferase (AST; EC 2.6.1.1) play a crucial role in transamination reactions and can be used as potential biomarkers to indicate hepatotoxicity and cellular damage. While histopathological studies in liver tissue require more time and expertise, simple and reliable biochemical analysis of ALT and AST can be used for a rapid assessment of tissue and cellular damage within 96 h. The main objective of this study was to determine the acute effects of arsenic and hexavalent chromium on the activity of ALT and AST in the Indian major carp, Labeo rohita for 24 h and 96 h. Significant increase in the activity of ALT (P < 0.01) from controls in arsenic exposed fish indicates serious hepatic damage and distress condition to the fish. However, no such significant changes were observed in chromium-exposed fish suggesting that arsenic is more toxic to the fish. These findings indicate that ALT and AST are candidate biomarkers for arsenic-induced hepatotoxicity in Labeo rohita.
authors.affiliation: Environmental and Molecular Toxicology Laboratory, Centre for Environment, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad-500 072, India. myvutukuru@yahoo.com
authors.forename: Sesha Srinivas
authors.initials: SS
authors.lastname: Vutukuru
authors.forename: N Arun
authors.initials: NA
authors.lastname: Prabhath
authors.forename: M
authors.initials: M
authors.lastname: Raghavender
authors.forename: Anjaneyulu
authors.initials: A
authors.lastname: Yerramilli
mesh_terms: D000410:Alanine Transaminase / Q000097:blood*; D000818:Animals; D001151:Arsenic / Q000633:toxicity*; D001219:Aspartate Aminotransferases / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D002347:Carps / Q000097:blood*; D002857:Chromium / Q000633:toxicity*; D007928:Lethal Dose 50; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D002857:Chromium; C074702:chromium hexavalent ion; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001151:Arsenic