[Immune response induced by Mycobacterium tuberculosis Rv2450 protein in mice].
OBJECTIVE
To evaluate humoral and cellular immune response induced by Rv2450 protein.


METHODS
Rv2450 protein was transferred to membrane and used to immunize C57BL/6 mice three times at 2 week interval. The spleen lymphocytes of the immunized mice were separated and the stimulation index (SI) was measured by MTT colorimetry and the levels of secreted IFN-gamma, IL-10 and IL-12 upon antigen-specific stimulation was detected by ELISA.


RESULTS
The titer of specific antibody in sera of the immunized C57BL/6 mice was 1:3 200. The SI of Rv2450 protein immunized group (3.76 +/- 0.19) was significantly higher than that of saline immunized group (0.89+/-0.17). The IFN-gamma, IL-10, IL-12 levels in culture supernatant of spleen lymphocytes from the immunized mice was (1 740 +/- 19) ng/L, (678 +/- 15) ng/L and (469 +/- 13) ng/L respectively, significantly different from that of saline-immunized group.


CONCLUSIONS
Rv2450 protein can be used as a candidate for the new TB vaccine.
Xue
Ying
Y
Department of Microbiology, Fourth Military Medical University, Xi'an, China. x.ying@yeah.net
Bai
Yin-Lan
YL
Gao
Hui
H
Wang
Li-Mei
LM
Xu
Zhi-Kai
ZK
D000818:Animals; D000917:Antibody Formation; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007106:Immune Sera / Q000032:analysis / Q000276:immunology; D007111:Immunity, Cellular; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D016207:Cytokines; D007106:Immune Sera; D008565:Membrane Proteins; C527541:Rv2560 protein, Mycobacterium tuberculosis