Conjugation to increase treatment volume during local therapy: a case study with PEGylated camptothecin.
Controlled release of chemotherapy drugs from polymer implants placed directly at the tumor site is a proven method for treatment of cancers of the brain. Although this method provides high doses of drug at the tumor site, the drug does not penetrate far enough into the brain for optimum treatment in most cases. Rapid drug elimination leads to more than a 10-fold drop in concentration within 2 mm of the implant. Conjugation to water-soluble polymers, such as poly(ethylene glycol) (PEG) or dextran, has the potential to increase drug distribution in the brain. We have recently PEGylated the chemotherapy drug camptothecin and found a large increase in the extent of distribution of camptothecin in the rat brain, but most of the drug in tissue was in the less-active conjugated form. Stability of the conjugation bond, activity of the drug-polymer conjugate, solubility of the conjugate relative to the drug, and molecular weight of the polymer must all be considered in the design of a conjugate to maximize drug distribution. Therefore, to optimize the PEGylated system, we have developed a pharmacokinetic model to determine the relative importance of parameters involved in the distribution of drug-polymer conjugates after release from a polymer implant. Our modeling shows that PEGylation has the potential to increase treatment distances to more than a centimeter, which may be sufficient to prevent the recurrence of human brain tumors.
Haverstick
Kraig
K
Department of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY, USA.
Fleming
Alison
A
Mark Saltzman
W
W
D000818:Animals; D001921:Brain / Q000187:drug effects; D002166:Camptothecin / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry; D006868:Hydrolysis; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry*; D051381:Rats
D004337:Drug Carriers; D011092:Polyethylene Glycols; D002166:Camptothecin