Effects of cultivation conditions on the diversity of microbes involved in the conversion of rice straw to fodder.
To confirm the optimum cultivation conditions for analyzing lactic acid bacterial communities and to provide the cultivation foundation for lactic acid bacterial communities that were used to convert straw into fodder, fermented rice straw was inoculated into 13 different broths. After 48 h of cultivation, pH values, volatile products, and microbial diversity were analyzed. Except for LAB broth, the pH values of the other broths could decrease to approximately 4.5. GC/MS analysis showed that lactic acid in Tomato MRS broth, MRS broth, LAB broth, and Tomato juice broth was higher than that in the other broths. DNA concentration analysis showed that the counts of microbes in Tomato MRS broth were 2.5 times of those in other broths and that tomato juice favored the reproduction of the microbes. Denaturing gradient gel electrophoresis (DGGE) analysis showed that the number of lactic acid bacterial species in HYA broth, Tomato juice broth, and Tomato MRS broth were higher than those in the other broths.
Yang
Hong-yan
HY
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China. yangdier@gmail.com
Gao
Li-juan
LJ
Wang
Xiao-fen
XF
Wang
Wei-dong
WD
Cui
Zong-jun
ZJ
D001483:Base Sequence; D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D012275:Oryza / Q000382:microbiology*; D013045:Species Specificity; D014835:Volatilization
D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial