Characterization of ScORK28, a transmembrane functional protein receptor kinase predominantly expressed in ovaries from the wild potato species Solanum chacoense.
Solanum chacoense ovule receptor kinase 28 (ScORK28) was found among 30 receptor kinases from an ovule cDNA library enriched for weakly expressed mRNAs. This LRR-RLK displayed high level of tissue specificity at the RNA and protein levels and was predominantly expressed in female reproductive tissues. Protein expression analyses in planta revealed that ScORK28 was N-glycosylated and ScORK28::GFP fusion analyses showed that it was localized at the plasma membrane. Bacterial expression of ScORK28 catalytic domain followed by kinase activity assays revealed that ScORK28 is an active Mg2+-dependent protein kinase and that the juxtamembrane domain is necessary for kinase activity.
Germain
Hugo
H
Institut de Recherche en Biologie Végétale (IRBV), Département de Sciences Biologiques, Université de Montréal, 4101 Sherbrooke Est, Montréal, QC, Canada H1X 2B2.
Houde
Josée
J
Gray-Mitsumune
Madoka
M
Sawasaki
Tatsuya
T
Endo
Yaeta
Y
Rivoal
Jean
J
Matton
Daniel P
DP
D000595:Amino Acid Sequence; D020134:Catalytic Domain; D002462:Cell Membrane / Q000201:enzymology*; D004926:Escherichia coli / Q000235:genetics; D008274:Magnesium / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D011494:Protein Kinases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D032322:Solanum / Q000201:enzymology* / Q000235:genetics
D008565:Membrane Proteins; D010940:Plant Proteins; D011494:Protein Kinases; D008274:Magnesium