title: A multi-fluorescent MDA435 cell line for mitosis inhibitor studies: simultaneous visualization of chromatin, microtubules, and nuclear envelope in living cells.
abstract: As a part of our efforts to create a pre-made multi-fluorescent cell library for various cytological assays, we made a triple-fluorescent cell line in which microtubules, chromosomes, and nuclear envelopes were visualized for simultaneous observation of spindle structure and chromosome distribution in living cells. Pilot experiments with microtubule-disturbing drugs showed the advantages of this cell line in mitosis inhibitor studies.
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Fukada
authors.forename: Kaori
authors.initials: K
authors.lastname: Senda-Murata
authors.forename: Kumi
authors.initials: K
authors.lastname: Nishida
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakaushi
authors.forename: Harumi
authors.initials: H
authors.lastname: Minamikawa
authors.forename: Masaya
authors.initials: M
authors.lastname: Dotsu
authors.forename: Shigenori
authors.initials: S
authors.lastname: Oka
authors.forename: Kenji
authors.initials: K
authors.lastname: Sugimoto
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D002843:Chromatin; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D008870:Microtubules; D008938:Mitosis / Q000187:drug effects*; D009685:Nuclear Envelope; D017239:Paclitaxel / Q000494:pharmacology; D010957:Plasmids
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002843:Chromatin; D005456:Fluorescent Dyes; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D017239:Paclitaxel