title: [Screening for the microdeletions of azoospermia factor on the Y chromosome in male infertile patients from Guangzhou].
abstract: OBJECTIVE
  To investigate the relationship between microdeletion of azoospermia factor (AZF) and male infertility.
  
  
  METHODS
  Multiplex PCR was used to detect Y chromosome microdeletion in AZFa, AZFb and AZFc in 103 cases of idiopathic azoospermia, 72 cases of severe idiopathic oligozoospermia, and 60 healthy male controls.
  
  
  RESULTS
  No microdeletion was found in 60 controls. Y chromosome microdeletion was found in 19 of 175 azoospermia patients, the total prevalence rate of microdeletion was 10.9%. There were 15 cases (11 for azoospermia, 4 for severe oligozoospermia) in AZFc (8.6%), 3 cases (1 for azoospermia, 2 for severe oligozoospermia) in AZFb+c (1.7%), 1 case (azoospermia) in AZFa+b+c (0.6%). According to statistics, the difference of microdeletion rate between two groups was significant(P < 0.01).
  
  
  CONCLUSIONS
  Y chromosome microdeletions is an important reason of azoospermia. Screening of Y chromosome microdeletions for azoospermia patients before intracytoplasmic sperm injection treatment is essential.
authors.affiliation: Center for Reproductive Medicine, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, 510080 PR China. liuyang20030901@yahoo.com.cn
authors.forename: Ya-feng
authors.initials: YF
authors.lastname: Liu
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Ou
authors.forename: Can-quan
authors.initials: CQ
authors.lastname: Zhou
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.forename: Zi-lian
authors.initials: ZL
authors.lastname: Wang
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics; D053713:Azoospermia / Q000175:diagnosis / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005260:Female; D056426:Genetic Loci; D005820:Genetic Testing; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009845:Oligospermia / Q000175:diagnosis / Q000235:genetics; D029589:Seminal Plasma Proteins / Q000235:genetics*
chemical_list: D029589:Seminal Plasma Proteins