Low cytotoxicity effect of dendrosome as an efficient carrier for rotavirus VP2 gene transferring into a human lung cell line : dendrosome, as a novel intranasally gene porter.
The efficiency of dendrosome (a gene porter) was assessed in transferring recombinant human rotavirus VP2 cDNA into A549, a human lung cell line. After gene transferring, transmission electron microscopy showed core-like particles (CLPs) formation in the transfected cells both with dendrosome and lipofectamine porters. In addition, western blotting analysis showed that the expression of VP2 gene was almost equal in the dendrosome and lipofectamine-transfected cells. Also, the cytotoxicity studies revealed that dendrosome had a lower cytotoxicity than lipofectamine. Therefore, our study may introduce dendrosome as a possible carrier for gene transferring into the human lung cell line, especially, for intranasally administration of DNA vaccines.
Pourasgari
Farzaneh
F
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. pourasgari@ibb.ut.ac.ir
Ahmadian
Shahin
S
Salmanian
Ali Hatef
AH
Sarbolouki
Mohammad Nabi
MN
Massumi
Mohammad
M
D036022:Capsid Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival; D004337:Drug Carriers / Q000737:chemistry*; D018014:Gene Transfer Techniques; D006801:Humans; D008055:Lipids / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D019444:Vaccines, DNA / Q000378:metabolism*; D014771:Virion / Q000648:ultrastructure
D036022:Capsid Proteins; D004337:Drug Carriers; D008055:Lipids; C086724:Lipofectamine; C442545:VP2 protein, Rotavirus; D019444:Vaccines, DNA