Nucleotide sequence of a G/11 family xylanase encoding gene in Scytalidium thermophilum.
The nucleotide sequence of the xylanase encoding gene in Scytalidium thermophilum Af101-3 was determined. The gene encodes a family G/11 xylanase, and the coding region is interrupted by a 72 bp intron. Transcription of the gene was investigated by reverse transcription PCR (RT-PCR). Transcription of the gene was not affected by the presence of 2% glucose in the medium. Xylanase production in S. thermophilum Af101-3 was also affected by concentration of glucose in the medium (modified Czapek's supplemented with 2% corn cob powder and 0.1% glucose). Therefore, xylanase expression in this fungus may not be regulated by the carbon source in the medium.
Boonlue
Sophon
S
Department of Bioresource Development, Hiroshima Prefectural University, Hiroshima, Japan.
Aimi
Tadanori
T
Kitamoto
Yutaka
Y
Morinaga
Tsutomu
T
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal / Q000235:genetics*; D005947:Glucose / Q000494:pharmacology; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014995:Xylosidases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
D014995:Xylosidases; D005947:Glucose