Response of Desulfovibrio vulgaris to alkaline stress.
The response of exponentially growing Desulfovibrio vulgaris Hildenborough to pH 10 stress was studied using oligonucleotide microarrays and a study set of mutants with genes suggested by microarray data to be involved in the alkaline stress response deleted. The data showed that the response of D. vulgaris to increased pH is generally similar to that of Escherichia coli but is apparently controlled by unique regulatory circuits since the alternative sigma factors (sigma S and sigma E) contributing to this stress response in E. coli appear to be absent in D. vulgaris. Genes previously reported to be up-regulated in E. coli were up-regulated in D. vulgaris; these genes included three ATPase genes and a tryptophan synthase gene. Transcription of chaperone and protease genes (encoding ATP-dependent Clp and La proteases and DnaK) was also elevated in D. vulgaris. As in E. coli, genes involved in flagellum synthesis were down-regulated. The transcriptional data also identified regulators, distinct from sigma S and sigma E, that are likely part of a D. vulgaris Hildenborough-specific stress response system. Characterization of a study set of mutants with genes implicated in alkaline stress response deleted confirmed that there was protective involvement of the sodium/proton antiporter NhaC-2, tryptophanase A, and two putative regulators/histidine kinases (DVU0331 and DVU2580).
Stolyar
Sergey
S
University of Washington, Department of Civil and Environmental Engineering, 616 NE North Lake Place, Box 355014, Seattle, WA 98195-2700, USA. sstolyar@washington.edu
He
Qiang
Q
Joachimiak
Marcin P
MP
He
Zhili
Z
Yang
Zamin Koo
ZK
Borglin
Sharon E
SE
Joyner
Dominique C
DC
Huang
Katherine
K
Alm
Eric
E
Hazen
Terry C
TC
Zhou
Jizhong
J
Wall
Judy D
JD
Arkin
Adam P
AP
Stahl
David A
DA
D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000235:genetics; D000468:Alkalies / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D016969:Desulfovibrio vulgaris / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D005407:Flagella / Q000235:genetics; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator; D000071677:Histidine Kinase; D018832:Molecular Chaperones / Q000096:biosynthesis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010447:Peptide Hydrolases / Q000096:biosynthesis / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics / Q000502:physiology; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000502:physiology; D014367:Tryptophan Synthase / Q000096:biosynthesis / Q000235:genetics; D014368:Tryptophanase / Q000235:genetics / Q000502:physiology
D000468:Alkalies; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018832:Molecular Chaperones; D017923:Sodium-Hydrogen Exchangers; D011494:Protein Kinases; D000071677:Histidine Kinase; D010447:Peptide Hydrolases; D000251:Adenosine Triphosphatases; D014368:Tryptophanase; D014367:Tryptophan Synthase