Production of acetone butanol (AB) from liquefied corn starch, a commercial substrate, using Clostridium beijerinckii coupled with product recovery by gas stripping.
A potential industrial substrate (liquefied corn starch; LCS) has been employed for successful acetone butanol ethanol (ABE) production. Fermentation of LCS (60 g l(-1)) in a batch process resulted in the production of 18.4 g l(-1) ABE, comparable to glucose: yeast extract based medium (control experiment, 18.6 g l(-1) ABE). A batch fermentation of LCS integrated with product recovery resulted in 92% utilization of sugars present in the feed. When ABE was recovered by gas stripping (to relieve inhibition) from the fed-batch reactor fed with saccharified liquefied cornstarch (SLCS), 81.3 g l(-1) ABE was produced compared to 18.6 g l(-1) (control). In this integrated system, 225.8 g l(-1) SLCS sugar (487 % of control) was consumed. In the absence of product removal, it is not possible for C. beijerinckii BA101 to utilize more than 46 g l(-1) glucose. A combination of fermentation of this novel substrate (LCS) to butanol together with product recovery by gas stripping may economically benefit this fermentation.
Ezeji
Thaddeus C
TC
Biotechnology and Bioengineering Group, Department of Food Science and Human Nutrition, University of Illinois, Urbana, IL 61801, USA. ezeji.1@osu.edu
Qureshi
Nasib
N
Blaschek
Hans P
HP
D000096:Acetone / Q000378:metabolism; D000440:Butanols / Q000378:metabolism; D046970:Clostridium beijerinckii / Q000201:enzymology*; D000431:Ethanol / Q000378:metabolism; D005740:Gases / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods*; D013213:Starch / Q000378:metabolism*
D000440:Butanols; D005740:Gases; D000096:Acetone; D000431:Ethanol; D013213:Starch