Translocation of annexin B1 in response to the stimulation of PMA and ionomycin in cervical cancer cells.
Annexin B1 is a novel member of the annexin superfamily which was isolated from a Cysticercus cellulosae cDNA library. To investigate the physiological roles of annexin B1, we firstly performed immunohistochemical analysis on frozen Cysticercus cellulosae sections and found that annexin B1 was present not only in the tegument of the bladder wall, but also in the host-derived inflammatory layer; In addition, ELISA analysis revealed that annexin B1 could be detected in the cystic fluid of Cysticercus cellulosae and the sera of pigs with cysticercosis. These findings indicated that annexin B1 might be a secretary protein. We further constructed a pEGFP-annexin B1 plasmid and transfected it into SiHa cells. We found that GFP-annexin B1 was stimulated to translocate to the plasma membrane by phorbol 12-myristate 13-acetate (PMA). By contrast, it was induced to distribute at the plasma and nuclear membranes by treatment with calcium ionophore ionomycin. PMA increased annexin B1 membrane binding, which might facilitate exocytosis. Moreover, translocation of the protein to the plasma and nuclear membranes after stimulated by ionomycin, was predicted to be related to an additional function.
Huang
Jing-Jing
JJ
Institute of Molecular Genetics and Engineering, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, PR China.
Yan
Hong-Li
HL
Gao
Yuan-Jian
YJ
Sun
Shu-Han
SH
He
Yan
Y
Ding
Fei-Xiang
FX
Mei
Qian
Q
Xue
Geng
G
D000818:Animals; D017302:Annexins / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D003551:Cysticercosis / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D005260:Female; D015801:Helminth Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D015759:Ionomycin / Q000494:pharmacology*; D010703:Phorbol Esters / Q000494:pharmacology*; D013552:Swine; D041201:Taenia solium / Q000378:metabolism; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000378:metabolism
D017302:Annexins; D015801:Helminth Proteins; D010703:Phorbol Esters; C449006:annexin B1, Taenia solium; D015759:Ionomycin; C032974:12-O-retinoylphorbol-13-acetate; D002118:Calcium