Effect of different growth factors on human osteoblasts activities: a possible application in bone regeneration for tissue engineering.
Cultured human primary osteoblasts reproduce the phenotypic differentiation and maturation of cells in vivo. We have investigated the influence of three isoforms of transforming growth factor beta (TGF-beta1, TGF-beta2 and TGF-beta3), three fibroblast growth factors (FGF-2, FGF-4 and FGF-6) and the active metabolite of Vitamin D [1,25-(OH)(2)D3] on proliferation, alkaline phosphatase activity and mineralization of human osteoblasts during a period of 24 days of culture. TGF-beta isoforms and three FGFs examined have been proved to be inducers of osteoblasts proliferation (higher extent for TGF-beta and FGF-2) and inhibitors of alkaline phosphatase activity and osteoblasts mineralization. Combination of these growth factors with the active form of Vitamin D induced osteodifferentiation. In fact Vitamin D showed an additive effect on alkaline phosphatase activity and calcium content, induced by FGF-2 and TGF-beta in human osteoblast. These results highlight the potential of proliferating cytokines' combination with mineralizing agents for in vitro bone growth induction in bone tissue engineering.
Bosetti
Michela
M
Department of Clinical and Experimental Medicine, School of Medicine, University of Eastern Piedmont, Via Solaroli 17, 28100 Novara, Italy.
Boccafoschi
Francesca
F
Leigheb
Massimiliano
M
Cannas
Mario F
MF
D000469:Alkaline Phosphatase / Q000378:metabolism; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D002113:Calcification, Physiologic / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D005346:Fibroblast Growth Factors / Q000494:pharmacology* / Q000502:physiology; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D015398:Signal Transduction; D023822:Tissue Engineering; D016212:Transforming Growth Factor beta / Q000494:pharmacology* / Q000502:physiology; D014807:Vitamin D / Q000494:pharmacology* / Q000502:physiology
D016212:Transforming Growth Factor beta; D014807:Vitamin D; D005346:Fibroblast Growth Factors; D000469:Alkaline Phosphatase