Expression of AITR and AITR ligand in breast cancer patients.
Human activation-inducible TNF receptor (AITR) is a new member of the tumor necrosis factor family and expressed on peripheral blood mononuclear cells (PBMC) and leukocytes. Its ligand (AITRL) is expressed in endothelial cells. This study aimed to evaluate the presence and role of AITR and AITRL in patients with breast cancer. Expression of AITR and AITRL on PBMC and breast cancer cells was determined by flow cytometry, RT-PCR and fluorescence microscopy. Soluble (s) AITR and sAITRL were detected in serum of breast cancer patients by ELISA. AITR and AITRL were constitutively expressed in T cells, B cells, monocytes and monocyte-derived dendritic cells of breast cancer patients. AITRL was overexpressed in breast cancer cells. The levels of sAITRL were significantly increased in serum of breast cancer patients compared with the healthy control. This study suggests that AITR and AITRL may play an important role in tumor growth and survival in breast cancer.
Kim
Yon Seon
YS
Department of Surgery, Ulsan University Hospital, Ulsan University College of Medicine, Ulsan 682-714, Korea.
Jung
Hyo Won
HW
Choi
Ji
J
Kwon
Byung Sae
BS
Ham
Soo-Youn
SY
Jung
Ae Kyung
AK
Ko
Byung Kyun
BK
D000328:Adult; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism; D001943:Breast Neoplasms / Q000097:blood* / Q000175:diagnosis; D016022:Case-Control Studies; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism; D048069:Tumor Necrosis Factors / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D018124:Receptors, Tumor Necrosis Factor; C478967:TNFSF18 protein, human; D048069:Tumor Necrosis Factors