Inhibitory effect of compounds from Zingiberaceae species on human platelet aggregation.
Twelve compounds isolated from Alpinia mutica Roxb., Kaempferia rotunda Linn., Curcuma xanthorhiza Roxb., Curcuma aromatica Valeton and Zingiber zerumbet Smith (Family: Zingiberaceae) and three synthesized derivatives of xanthorrhizol were evaluated for their ability to inhibit arachidonic acid- (AA), collagen- and ADP-induced platelet aggregation in human whole blood. Antiplatelet activity of the compounds was measured in vitro by the Chrono Log whole blood aggregometer using an electrical impedance method. Among the compounds tested, curcumin from C. aromatica, cardamonin, pinocembrine and 5,6-dehydrokawain from A. mutica and 3-deacetylcrotepoxide from K. rotunda showed strong inhibition on platelet aggregation induced by AA with IC(50) values of less than 84 microM. Curcumin was the most effective antiplatelet compound as it inhibited AA-, collagen- and ADP-induced platelet aggregation with IC(50) values of 37.5, 60.9 and 45.7 microM, respectively.
Jantan
I
I
Department of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. ibj@medic.ukm.my
Raweh
S M
SM
Sirat
H M
HM
Jamil
S
S
Mohd Yasin
Y H
YH
Jalil
J
J
Jamal
J A
JA
D047188:Chalcones / Q000302:isolation & purification / Q000494:pharmacology; D003474:Curcumin / Q000302:isolation & purification / Q000494:pharmacology; D044950:Flavanones / Q000302:isolation & purification / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D011753:Pyrones / Q000302:isolation & purification / Q000494:pharmacology; D027343:Rhizome / Q000737:chemistry; D013329:Structure-Activity Relationship; D030521:Zingiberaceae / Q000737:chemistry*
D047188:Chalcones; D044950:Flavanones; D010936:Plant Extracts; D011753:Pyrones; C052297:5,6-dehydrokawain; C016063:pinocembrin; C436747:cardamonin; D003474:Curcumin