Approaches for analyzing the differential activities and functions of eIF4E family members.
The translational initiation factor eIF4E binds to the m(7)G-containing cap of mRNA and participates in recruitment of mRNA to ribosomes for protein synthesis. eIF4E also functions in nucleocytoplasmic transport of mRNA, sequestration of mRNA in a nontranslatable state, and stabilization of mRNA against decay in the cytosol. Multiple eIF4E family members have been identified in a wide range of organisms that includes plants, flies, mammals, frogs, birds, nematodes, fish, and various protists. This chapter reviews methods that have been applied to learn the biochemical properties and physiological functions that differentiate eIF4E family members within a given organism. Much has been learned to date about approaches to discover new eIF4E family members, their in vitro properties (cap binding, stimulation of cell-free translation systems), tissue and developmental expression patterns, protein-binding partners, and their effects on the translation or repression of specific subsets of mRNA. Despite these advances, new eIF4E family members continue to be found and new physiological roles discovered.
Rhoads
Robert E
RE
Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, USA.
Dinkova
Tzvetanka D
TD
Jagus
Rosemary
R
D000818:Animals; D017360:Arabidopsis / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000378:metabolism; D002846:Chromatography, Affinity; D039561:Eukaryotic Initiation Factor-4E / Q000235:genetics / Q000502:physiology*; D005453:Fluorescence; D013058:Mass Spectrometry; D046228:Microarray Analysis; D025941:Protein Interaction Mapping; D012333:RNA, Messenger / Q000502:physiology; D011817:Rabbits; D011994:Recombinant Proteins / Q000378:metabolism; D012156:Reticulocytes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D039561:Eukaryotic Initiation Factor-4E; D012333:RNA, Messenger; D011994:Recombinant Proteins