Free radical scavenging activity and secondary metabolites from in vitro cultures of Sanicula graveolens.
An in vitro propagation system was developed to obtain shoot and root cultures from the Andean spice Sanicula graveolens (Apiaceae). Propagation of shoots, roots and plantlets was achieved by the temporary immersion system. The free radical scavenging effect of the methanol/water (7:3 v/v) extracts was determined by the discoloration of the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH). Total phenolic, flavonoid, chlorogenic acid (CA) and quercetin 3-O-glucoside content in the samples was assessed by spectrophotometry and DAD-HPLC analysis, respectively. On a dry weight basis, the crude extracts showed total phenolic values ranging from 3.57 to 6.93%, with highest content for the root culture sample. Total flavonoid content ranged from 1.23 to 2.23% and was lower for the root culture. Chlorogenic acid and neochlorogenic acid were identified by TLC in all samples. Highest free radical scavenging effect was observed for the root culture which also presented the highest CA content. Two of the shoot culture samples, with similar IC50 values in the DPPH discoloration assay, also presented close quercetin-3-O-glucoside content.
Cheel
José
J
Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Universidad de Talca, Casilla 747, Talca, Chile.
Schmeda-Hirschmann
Guillermo
G
Jordan
Miguel
M
Theoduloz
Cristina
C
Rodríguez
Jaime A
JA
Gerth
André
A
Wilken
Dirk
D
D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D005612:Freeze Drying; D006834:Hydrazines; D010851:Picrates; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry / Q000502:physiology; D018520:Plant Shoots / Q000737:chemistry / Q000502:physiology; D018547:Plant Stems / Q000737:chemistry / Q000502:physiology; D018514:Plant Structures / Q000737:chemistry*; D030005:Sanicula / Q000737:chemistry* / Q000187:drug effects / Q000502:physiology; D017365:Spices
D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006834:Hydrazines; D010851:Picrates; D010937:Plant Growth Regulators; C004931:1,1-diphenyl-2-picrylhydrazyl