Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China.
A novel yellow-pigmented, non-motile, non-sporulating, catalase- and oxidase-positive, obligately aerobic, moderately halophilic, facultatively alkaliphilic Gram-positive coccus, strain YIM Y15(T), was isolated from a brine sample from a salt mine in Yunnan, south-west China. Strain YIM Y15(T) grew in the presence of 0.5-25 % (w/v) NaCl and at pH 6.0-10.0, with optimum growth at 8-10 % (w/v) NaCl and pH 8.0. It grew at 4-45 degrees C, with optimum growth at 37.0 degrees C. The major cellular fatty acids were anteiso-C(15 : 0) and iso-C(15 : 0). Menaquinone 6 (MK-6) was the major respiratory quinone. The cell wall contained Lys and Gly. The DNA G+C content was 46.2 mol%. Phylogenetic analyses based on 16S rRNA gene sequence comparisons revealed that strain YIM Y15(T) was a member of the genus Salinicoccus, with low 16S rRNA gene sequence similarity to the type strains of the five described species of the genus, Salinicoccus alkaliphilus JCM 11311(T) (sequence similarity 96.0 %), Salinicoccus roseus DSM 5351(T) (94.9 %), Salinicoccus jeotgali KCTC 13030(T) (94.8 %), Salinicoccus salsiraiae LMG 22840(T) (94.8 %) and Salinicoccus hispanicus DSM 5352(T) (94.7 %). Together with the phenotypic differences, these results supported the proposal of a novel species of the genus Salinicoccus, Salinicoccus kunmingensis sp. nov., with YIM Y15(T) (=DSM 17847(T) =CGMCC 1.6302(T)) as the type strain.
Chen
Yi-Guang
YG
College of Bio-resources and Environmental Science, Jishou University, Jishou, Hunan 416000, PR China.|Yunnan Institute of Microbiology and Key Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan 650091, PR China.
Cui
Xiao-Long
XL
Yunnan Institute of Microbiology and Key Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan 650091, PR China.
Pukall
Rüdiger
R
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7b, D-38124 Braunschweig, Germany.
Li
Hui-Ming
HM
Yunnan Institute of Microbiology and Key Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan 650091, PR China.
Yang
Ya-Ling
YL
Yunnan Salt & Chemical Industry Co., Ltd, Kunming, Yunnan 650091, PR China.
Xu
Li-Hua
LH
Yunnan Institute of Microbiology and Key Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan 650091, PR China.
Wen
Meng-Liang
ML
Yunnan Institute of Microbiology and Key Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan 650091, PR China.
Peng
Qian
Q
Yunnan Institute of Microbiology and Key Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan 650091, PR China.
Jiang
Cheng-Lin
CL
Yunnan Institute of Microbiology and Key Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan 650091, PR China.
D000332:Aerobiosis / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005998:Glycine / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008124:Locomotion / Q000502:physiology; D008239:Lysine / Q000032:analysis; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D040141:Staphylococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010860:Pigments, Biological; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D010088:Oxidoreductases; D002374:Catalase; D008239:Lysine; D005998:Glycine