Somatic cell count in milk of selenium-supplemented dairy cows after an intramammary challenge with Staphylococcus aureus.
The objective of this study was to evaluate the effect of selenium (Se) supplementation on milk somatic cell count (SCC) in dairy cows. Twelve multiparous Holstein-Friesian cows were fed a diet containing a suboptimal Se concentration (<0.05 ppm, dry basis) starting 2 months before calving. Supplemented cows (n=6) received a single s.c. injection of barium selenate (1 ml/50 kg BW) 45 days prior to calving, whereas control group was kept unsupplemented. Twenty weeks after calving, two mammary quarters (right side) of each cow were challenged with 205,000 cfu/ml of Staphylococcus aureus (strain Newbould 305). Blood was collected bi-weekly until day 150 of lactation for the analysis of blood glutathione peroxidase (GPx1; EC 1.11.1.9) activity. To re-isolate the challenging pathogen and to evaluate SCC, aseptic milk samples were collected daily starting on the day of challenge, and finishing 7 days after inoculation. Unsupplemented cows had a lower activity of GPx1 through the experiment (P<0.001). Natural log SCC (lnSCC) was higher in unsupplemented than Se-supplemented cows (P=0.04), showing evidence of significance after 5 days. Selenium supplementation of dairy cows fed a diet containing a suboptimal Se concentration, resulted in higher blood activity of GPx1, and lower mean lnSCC after an intramammary challenge with Staph. aureus.
Kruze
J
J
Department of Health Management, University of Prince Edward Island, Charlottetown, PE, Canada.
Ceballos
A
A
Stryhn
H
H
Mella
A
A
Matamoros
R
R
Contreras
P A
PA
Leyan
V
V
Wittwer
F
F
D000818:Animals; D002417:Cattle; D002452:Cell Count / Q000662:veterinary; D019587:Dietary Supplements; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D007113:Immunity, Innate; D008414:Mastitis, Bovine / Q000097:blood* / Q000276:immunology; D008892:Milk / Q000166:cytology*; D011247:Pregnancy; D012643:Selenium / Q000008:administration & dosage / Q000097:blood / Q000172:deficiency; D013203:Staphylococcal Infections / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000472:pathogenicity; D013997:Time Factors
D005979:Glutathione Peroxidase; D012643:Selenium