title: Genome-wide screen of Salmonella genes expressed during infection in pigs, using in vivo expression technology.
abstract: Pigs are a food-producing species that readily carry Salmonella but, in the great majority of cases, do not show clinical signs of disease. Little is known about the functions required by Salmonella to be maintained in pigs. We have devised a recombinase-based promoter-trapping strategy to identify genes with elevated expression during pig infection with Salmonella enterica serovar Typhimurium. A total of 55 clones with in vivo-induced promoters were selected from a genomic library of approximately 10,000 random Salmonella DNA fragments fused to the recombinase cre, and the cloned DNA fragments were analyzed by sequencing. Thirty-one genes encoding proteins involved in bacterial adhesion and colonization (including bcfA, hscA, rffG, and yciR), virulence (metL), heat shock (hscA), and a sensor of a two-component regulator (hydH) were identified. Among the 55 clones, 19 were isolated from both the tonsils and the intestine, while 23 were identified only in the intestine and 13 only in tonsils. High temperature and increased osmolarity were identified as environmental signals that induced in vivo-expressed genes, suggesting possible signals for expression.
authors.affiliation: Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Huang
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Leming
authors.forename: Mitsu
authors.initials: M
authors.lastname: Suyemoto
authors.forename: Craig
authors.initials: C
authors.lastname: Altier
mesh_terms: D000818:Animals; D010861:Fimbriae, Bacterial / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D007506:Iron-Sulfur Proteins / Q000235:genetics; D008070:Lipopolysaccharides / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D045522:Recombinases / Q000235:genetics; D012475:Salmonella / Q000235:genetics* / Q000472:pathogenicity; D012480:Salmonella Infections / Q000382:microbiology*; D013552:Swine / Q000382:microbiology*; D014774:Virulence / Q000235:genetics; D014805:Vitamin B 12 / Q000235:genetics
chemical_list: D007506:Iron-Sulfur Proteins; D008070:Lipopolysaccharides; D045522:Recombinases; D014805:Vitamin B 12