title: Variations in dominant antigen determinants of glutaraldehyde polymerized human, bovine and porcine hemoglobin.
abstract: In this study, immunogenicity of human hemoglobin (hHb), bovine hemoglobin (bHb), porcine hemoglobin (pHb) and their glutaradehyde polymerized derivatives (hPolyHb, bPolyHb and bPolyHb, respectively) were compared. The nature of the dominant antigen determinants of the chemically polymerized proteins was studied. Glutaraldehyde chemical reaction enhanced the immunogenicity of the hemoglobin derivatives. In mice, the extent of the enhancement was largely comparable among hPolyHb, bPolyHb and pPolyHb. Using the methods of semi-quantitative western blotting and quantitative protein array, it was found that most of the polycloncal antibodies raised in rodents against glutaraldehyde polymerized hemoglobin derivatives of human, bovine or porcine species only weakly or did not cross-react with the hemoglobin derivatives of the other two species, indicating that hPolyHb, bPolyHb and bPolyHb vary significantly in their dominant antigen determinants, despite very high degree of identity in their primary amino acid sequences and high similarity in their three dimensional structures.
authors.affiliation: Northwest University, Xi'an, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhu
authors.forename: Wei
authors.initials: W
authors.lastname: Chu
authors.forename: Tongwen
authors.initials: T
authors.lastname: Wang
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.forename: Daidi
authors.initials: D
authors.lastname: Fan
authors.forename: Ning
authors.initials: N
authors.lastname: Dan
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
mesh_terms: D000818:Animals; D000941:Antigens / Q000737:chemistry / Q000276:immunology*; D001802:Blood Substitutes / Q000737:chemistry*; D002417:Cattle; D018604:Epitope Mapping; D005260:Female; D006454:Hemoglobins / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology*; D006801:Humans; D007117:Immunization, Secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D000091402:Prohibitins; D020539:Sequence Analysis, Protein; D034421:Sus scrofa
chemical_list: D000941:Antigens; D001802:Blood Substitutes; D006454:Hemoglobins; C000717328:PHB protein, human; D000091402:Prohibitins; C037158:polyhemoglobin