Suggestive linkage to a neighboring region of IRF6 in a cleft lip and palate multiplex family.
Cleft lip and/or palate (CL/P) is a common congenital malformation with a complex etiology, as many genes and environmental factors have been shown to play a role in craniofacial development. We used a genetic mapping approach to analyze a family with multiplex CL/P. A genome-wide scan with a 10 kb single nucleotide polymorphism (SNP) chip followed by fine mapping with microsatellite markers in a CL/P multiplex family suggested linkage (maximum multipoint LOD score of 2.41) to a 6.5 Mb interval at 1q32.1-q32.2. This interval was close to, but excluded IRF6. Mutations in the IRF6 (1q32.2) cause syndromic forms of CL/P, and several association studies have shown that polymorphisms in and around IRF6 are associated with non-syndromic CL/P (NSCLP). However, in the family described here, IRF6 was excluded from the linkage interval. Sequencing of selected genes in the interval and comparative genome hybridization (CGH) did not reveal any mutations or genomic aberrations. Our data suggest that an unidentified CL/P gene, or a non-coding IRF6 regulatory element in this linkage interval may have caused CL/P in this family.
Jakobsen
Linda P
LP
Department of Cellular and Molecular Medicine, Panum Institute, Wilhelm Johannsen Centre for Functional Genome Research, Copenhagen, Denmark. linda@imbg.ku.dk
Ullmann
Reinhard
R
Kjaer
Klaus W
KW
Knudsen
Mary A
MA
Tommerup
Niels
N
Eiberg
Hans
H
D002971:Cleft Lip / Q000235:genetics*; D002972:Cleft Palate / Q000235:genetics*; D003718:Denmark; D005192:Family Health; D005260:Female; D008040:Genetic Linkage; D006801:Humans; D050835:Interferon Regulatory Factors / Q000235:genetics*; D008297:Male; D010375:Pedigree; D050436:Regulatory Elements, Transcriptional
C109803:IRF6 protein, human; D050835:Interferon Regulatory Factors