[Effects of testosterone on extracellular signal-regulated kinase 1/2 phosphorylation in human umbilical vein endothelial cells].
OBJECTIVE
To investigate the effects of testosterone on extracellular signal-regulated kinase l/2 ( ERK1/2) phosphorylation in human umbilical vein endothelial cells (HUVEC).


METHODS
Activations of ERK1/2 stimulated by physiological testosterone were detected by Western blotting in cultured HUVEC.


RESULTS
A rapid phosphorylation expression of ERK1/2 was observed by treatment of the HUVECs with 3 x 10(-8) mol/L testosterone, especially at 30 minutes. This phosphorylation was greatly inhibited by incubation with androgen receptor antagonist flutamide for 3 hours previously.


CONCLUSIONS
Testosterone at physiological concentrations induces the mitogen-activated protein kinase (MAPK, ERK1/2 and MEK1/2) phosphorylation within a short time, and flutamide could impair the process.
Jin
Hong
H
Department of Cardiology, the First Hospital Affiliated to Shantou University Medical College, Shantou, Guangdong 515041, China. cherry1970@sina.com.cn
Zhang
Xiao-ying
XY
Peng
Geng
G
Qiu
Wen-bing
WB
Wang
Dong-ming
DM
Li
Yu-guang
YG
D059002:Androgen Receptor Antagonists; D015153:Blotting, Western; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D011944:Receptors, Androgen / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
D059002:Androgen Receptor Antagonists; D011944:Receptors, Androgen; D013739:Testosterone; D048049:Extracellular Signal-Regulated MAP Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3