Characterization of protein arginine methyltransferases in porcine brain.
Protein arginine methylation is a posttranslational modification involved in various cellular functions including cell signaling, protein subcellular localization and transcriptional regulation. We analyze the protein arginine methyltransferases (PRMTs) that catalyze the formation of methylarginines in porcine brain. We fractionated the brain extracts and determined the PRMT activities as well as the distribution of different PRMT proteins in subcellular fractions of porcine brain. The majority of the type I methyltransferase activities that catalyze the formation of asymmetric dimethylarginines was in the cytosolic S3 fraction. High specific activity of the methyltransferase was detected in the S4 fraction (high-salt stripping of the ultracentrifugation precipitant P3 fraction), indicating that part of the PRMT was peripherally associated with membrane and ribosomal fractions. The amount and distribution of PRMT1 are consistent with the catalytic activity. The elution patterns from gel filtration and anion exchange chromatography also indicate that the type I activity in S3 and S4 are mostly from PRMT1. Our results suggest that part of the type I arginine methyltransferases in brains, mainly PRMT1, are sequestered in an inactive form as they associated with membranes or large subcellular complexes. Our biochemical analyses confirmed the complex distribution of different PRMTs and implicate their regulation and catalytic activities in brain.
Hung
Chien-Jen
CJ
Department of Biomedical Sciences, Chung Shan Medical University, Taichung, Taiwan, ROC.
Chen
Da-Huang
DH
Shen
Yi-Ting
YT
Li
Yi-Chen
YC
Lin
Yi-Wei
YW
Hsieh
Mingli
M
Li
Chuan
C
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004789:Enzyme Activation / Q000187:drug effects; D008745:Methylation / Q000187:drug effects; D011189:Potassium Chloride / Q000494:pharmacology; D011484:Protein-Arginine N-Methyltransferases / Q000302:isolation & purification* / Q000378:metabolism*; D013552:Swine
D011189:Potassium Chloride; D011484:Protein-Arginine N-Methyltransferases