Isolation and amplification of genomic DNA from recalcitrant dried berries of black pepper (Piper nigrum L.)--a medicinal spice.
Black pepper is an important medicinal spice traded internationally. The extraction of high quality genomic DNA for PCR amplification from dried black pepper is challenging because of the presence of the exceptionally large amount of oxidized polyphenolic compounds, polysaccharides and other secondary metabolites. Here we report a modified hexadecyl trimethyl ammonium bromide (CTAB) protocol by incorporating potassium acetate and a final PEG precipitation step to isolate PCR amplifiable genomic DNA from dried and powdered berries of black pepper. The protocol has trade implication as it will help in the PCR characterization of traded black peppers from different countries.
Dhanya
K
K
Division of Crop Improvement and Biotechnology, Indian Institute of Spices Research, Calicut, Kerala, 673012, India.
Kizhakkayil
Jaleel
J
Syamkumar
S
S
Sasikumar
B
B
D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification*; D003890:Desiccation; D005638:Fruit / Q000235:genetics*; D005784:Gene Amplification / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics*; D029222:Piper nigrum / Q000235:genetics*
D018744:DNA, Plant