Cellulose and hemicelluloses recovery from grape stalks.
In this work, two mild chemical fractionation procedures were compared to separate and recover lignocellulosic components from grape stalks. The first method consisted of mild acid hydrolysis for hemicelluloses separation, followed by an alkaline/oxidative step for lignin solubilization, while in the second method the acid hydrolysis was preceded by an alkali steeping phase. Influence of the length of the first step of both methods (from 2 to 24 h) on monosaccharides and cellulose yields was investigated. The first method allowed a higher sugar recovery for longer times, and a slightly lower amount of cellulose. Cellulose residues from both the methods were comparable for cellulose content and thermal profile (studied by differential scanning calorimetry). Acid hydrolysis of the first step was carried out also in autoclave, showing that xylan degradation could be described by a first order kinetics where at higher temperature the presence of a fast reaction and a slow reacting fraction must be accounted for.
Spigno
Giorgia
G
Institute of Oenology and Food Engineering, Università Cattolica del Sacro Cuore, Via Emilia Parmense, 84-29100 Piacenza, Italy. giorgia.spingo@unicatt.it
Pizzorno
Tiziana
T
De Faveri
Dante Marco
DM
D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002152:Calorimetry, Differential Scanning / Q000379:methods; D002241:Carbohydrates / Q000737:chemistry; D002482:Cellulose / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D005285:Fermentation; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry; D015233:Models, Statistical; D010100:Oxygen / Q000737:chemistry; D010944:Plants / Q000378:metabolism*; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification*; D013997:Time Factors; D027843:Vitis
D002241:Carbohydrates; D011134:Polysaccharides; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D010100:Oxygen