[Study on the optimum extracting conditions of total alkaloids and aconitine in Aconitum szechenyianum Gay].
OBJECTIVE
To optimize the conditions for extractions of total alkaloids and aconitine from Aconitum szechenyianum Gay.


METHODS
Using L16 (4(5)) orthogonal test and univariant methods, the exrtaction conditions of total alkaloids aconitine in Aconitum szechenyianum Gay. were optimized under the guidance of the content determination of total alkaloids with bromophemol blue colorimetry and aconitine with HPLC method, respectively.


RESULTS
The obtained optimum condition of total alkaloids was that the material was refluxed in 6 times (m/v) acidic alcohol solution( alcohol: pH 3.0 HAc = 85:15, v/v) for 1 h with 3 times. The condition of aconitine was that the material was refluxed in 4 times (m/v) acidic alcohol solution (alcohol: pH 3. p HAc = 15:85, v/v) for 0.5 h with 3 times. Contents of total alkaloids and aconitine in Aconitum szechenyianum Gay. were 0.980% and 0.109%, respectively.


CONCLUSIONS
Through the test and verify, the optimum extraction methods of total alkaloids and aconitine are rational.
Kang
Xue-Qin
XQ
School of Chemistry & Material Science of Shaanxi Normal University, Xi'an 710062, China.
Fan
Zhi-Chao
ZC
Zhang
Zhi-Qi
ZQ
D000157:Aconitine / Q000032:analysis / Q000302:isolation & purification; D000470:Alkaloids / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000431:Ethanol / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D029626:Ranunculaceae / Q000737:chemistry*; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors
D000470:Alkaloids; D012997:Solvents; D000431:Ethanol; D000157:Aconitine