[Inhibition of Sorbaria sorbifolia on proliferarion of hepatoma HepG-2 cell line].
OBJECTIVE
To investigate the inhibition of Sorbaria sorbifolia on proliferation hepatoma HepG-2 cells, and to explore the antineoplastic mechanism of Sorbaria sorbifolia.


METHODS
MTT assay was used to examine the effect of Sorbaria sorbifolia on the proliferation of HepG-2 cell and flow cytometry was used for the cell cycle distribution. Apoptosis of HepG-2 cell was investigated further by means of inverted microscope, HE staining, transmission electron microscopy and expression of apoptosis-associated gene bcl-2 and p53 were determined by immunohistochemical method.


RESULTS
Sorbaria sorbifolia inhibited the proliferation of HepG-2 cell and the effects were in a time-and-concentration dependent manner. Sorbaria sorbifolia could also induce apoptosis, the G2/M phase was arrested and the ratio of apoptosis was significantly difference with that of control group, respectively. The expressions of apoptosis-associated gene bcl-2 and p53 had changed significantly, respectively.


CONCLUSIONS
Sorbaria sorbifolia inhibits proliferation of HepG-2 cells via induction of apoptosis and cell cycle arrest.
Zhang
Xue-Wu
XW
Basic Medical College, Yanbian University, Yanji 133000, China.
Cui
Chang-Xu
CX
Chen
Li-Yan
LY
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D010946:Plants, Medicinal / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D027824:Rosaceae / Q000737:chemistry*; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53