Altererythrobacter epoxidivorans gen. nov., sp. nov., an epoxide hydrolase-active, mesophilic marine bacterium isolated from cold-seep sediment, and reclassification of Erythrobacter luteolus Yoon et al. 2005 as Altererythrobacter luteolus comb. nov.
A novel marine bacterium, strain JCS350(T), was isolated from marine sediment samples collected from a cold-seep area. The 16S rRNA gene sequence of the isolate showed high similarity to that of Erythrobacter luteolus SW-109(T) (95.9 % sequence similarity). Lower 16S rRNA gene sequence similarities were shown to other members of the genus Erythrobacter (94.6-95.4 %) and members of the genus Porphyrobacter (94.5-95.2 %). Phylogenetic analysis with all members of the family Erythrobacteraceae and several members of the family Sphingomonadaceae revealed that the isolate formed a phyletic line with [Erythrobacter] luteolus that was distinct from other members of the family Erythrobacteraceae. The dominant fatty acids of strain JCS350(T) were 18 : 1omega7c, 16 : 1omega7c and cyclopropane 17 : 0. The major respiratory quinone was ubiquinone 10. The DNA G+C content was 54.5 mol%. The isolate did not contain bacteriochlorophyll a. Optimal growth required the presence of 2 % (w/v) NaCl with either 0.18 % CaCl(2) or 0.59 % MgCl(2), at pH 6.5 and at 35 degrees C. On the basis of the evidence of this polyphasic taxonomic study, strain JCS350(T) should be classified in a novel genus and species in the family Erythrobacteraceae, for which the name Altererythrobacter epoxidivorans gen. nov., sp. nov. is proposed. The misclassified species [Erythrobacter] luteolus is transferred to the new genus as Altererythrobacter luteolus comb. nov. The type strain of Altererythrobacter epoxidivorans is JCS350(T) (=KCCM 42314(T) =JCM 13815(T)) and the type strain of Altererythrobacter luteolus is SW-109(T) (=KCTC 12311(T) =JCM 12599(T)).
Kwon
Kae Kyoung
KK
Marine Biotechnology Research Center, Korea Ocean Research & Development Institute, PO Box 29, Ansan 425-600, Republic of Korea.
Woo
Jung-Hee
JH
Marine Biotechnology Research Center, Korea Ocean Research & Development Institute, PO Box 29, Ansan 425-600, Republic of Korea.
Yang
Sung-Hyun
SH
Marine Biotechnology Research Center, Korea Ocean Research & Development Institute, PO Box 29, Ansan 425-600, Republic of Korea.
Kang
Ji-Hyun
JH
Marine Biotechnology Research Center, Korea Ocean Research & Development Institute, PO Box 29, Ansan 425-600, Republic of Korea.
Kang
Sung Gyun
SG
Marine Biotechnology Research Center, Korea Ocean Research & Development Institute, PO Box 29, Ansan 425-600, Republic of Korea.
Kim
Sang-Jin
SJ
Marine Biotechnology Research Center, Korea Ocean Research & Development Institute, PO Box 29, Ansan 425-600, Republic of Korea.
Sato
Takako
T
Extremobiosphere Research Center, JAMSTEC, 2-15 Natsushima-cho, Yokosuka City, Kanagawa 237-0061, Japan.
Kato
Chiaki
C
Extremobiosphere Research Center, JAMSTEC, 2-15 Natsushima-cho, Yokosuka City, Kanagawa 237-0061, Japan.
D020561:Alphaproteobacteria / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D025541:Bacteriochlorophyll A / Q000032:analysis; D001482:Base Composition; D002122:Calcium Chloride / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004851:Epoxide Hydrolases / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D015636:Magnesium Chloride / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
D025541:Bacteriochlorophyll A; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D015636:Magnesium Chloride; D012965:Sodium Chloride; D004851:Epoxide Hydrolases; D002122:Calcium Chloride