Disruption of mouse poly(A) polymerase mGLD-2 does not alter polyadenylation status in oocytes and somatic cells.
The elongation of poly(A) tails in cytoplasm is essential for oogenesis and early embryogenesis in Xenopus laevis. mGLD-2 is a mouse homologue of Xenopus cytoplasmic poly(A) polymerase xGLD-2. We found an association of mGLD-2 with cytoplasmic polyadenylation components, CPEB and CPSF described in Xenopus oocytes. To clarify the role of mGLD-2 in mouse, we produced an mGLD-2 disrupted mouse line by homologous recombination. In spite of the ubiquitous expression of mGLD-2, the disrupted mice were apparently normal and healthy. Moreover, it was demonstrated that mGLD-2 disruption did not affect the poly(A) tail elongation in oocytes using reporter RNAs. Coincide with these observations, the maturation of the oocytes was normal and the mice were fertile. Thus mGLD-2 is dispensable for full-term development and oogenesis. Our results also indicate that there is another source of cytoplasmic poly(A) polymerase in mouse.
Nakanishi
Tomoko
T
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan. tomo@grape.med.tottori-u.ac.jp
Kumagai
Satoshi
S
Kimura
Masanori
M
Watanabe
Hiromi
H
Sakurai
Takayuki
T
Kimura
Minoru
M
Kashiwabara
Shin-ichi
S
Baba
Tadashi
T
D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D008099:Liver / Q000378:metabolism*; D051379:Mice; D009865:Oocytes / Q000378:metabolism*; D026723:Polyadenylation / Q000187:drug effects*; D011062:Polynucleotide Adenylyltransferase / Q000235:genetics* / Q000378:metabolism
D011062:Polynucleotide Adenylyltransferase