title: Pyrene-perylene as a FRET pair coupled to the N2'-functionality of 2'-amino-LNA.
abstract: Detection of nucleic acid hybridization via fluorescence resonance energy transfer (FRET) using pyren-1-ylmethyl and perylen-3-ylmethyl N2'-functionalized 2'-amino-LNA nucleosides incorporated into oligonucleotides exhibited a clear distance dependence of the FRET efficiency, ranging from below 10% when the fluorophores were approximately 40A apart to approximately 90% when the fluorophores were in close proximity.
authors.affiliation: Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.
authors.forename: Dorthe
authors.initials: D
authors.lastname: Lindegaard
authors.forename: Andreas S
authors.initials: AS
authors.lastname: Madsen
authors.forename: Irina V
authors.initials: IV
authors.lastname: Astakhova
authors.forename: Andrei D
authors.initials: AD
authors.lastname: Malakhov
authors.forename: B Ravindra
authors.initials: BR
authors.lastname: Babu
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Korshun
authors.forename: Jesper
authors.initials: J
authors.lastname: Wengel
mesh_terms: D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry*; D010569:Perylene / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011721:Pyrenes; D010569:Perylene; C030984:pyrene