[Comparison of the binding affinity of LAIR-1(CD305) and LAIR-2(CD306) with their ligands].
OBJECTIVE
To compare the binding affinity of LAIR-1 and LAIR-2 with their ligands.


METHODS
The ligand-expressing cells were incubated with the LAIR-1 and/or LAIR-2 fusion proteins at different concentration at the same time or in succession. Then the interaction changes and competition were measured by influorescent staining and flow cytometric analysis with the specific mAbs against LAIR-1 or LAIR-2.


RESULTS
The membrane ligand for LAIR-1 and LAIR-2 was expressed extensively and ligand recognition was of cross-species. LAIR-2 blocked the interaction of LAIR-1 with its ligand but LAIR-1 didn't block the interaction of LAIR-2 with its ligand.


CONCLUSIONS
LAIR-1 and LAIR-2 probably bind to the same ligand with different affinity, which provides some significant evidence for investigating the molecular mechanisms of LAIR-1 family in modulating immune response.
Xu
Xiao-Guang
XG
Transplantation Center, Second Affiliated Hospital, General Hospital of PLA, Beijing, China. xg_xu2000@yahoo.com
Wang
Chun-Yan
CY
Xie
Xin
X
Zhang
Yuan
Y
Han
Wei-Ning
WN
Cai
Ming
M
Shi
Bing-Yi
BY
Jin
Bo-Quan
BQ
D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006801:Humans; D008024:Ligands; D011485:Protein Binding; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D014162:Transfection
C431503:LAIR-2 receptor; D008024:Ligands; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins; C108073:leukocyte-associated immunoglobulin-like receptor 1