title: Heat shock response in CHO mammalian cells is controlled by a nonlinear stochastic process.
abstract: In many biological systems, the interactions that describe the coupling between different units in a genetic network are nonlinear and stochastic. We study the interplay between stochasticity and nonlinearity using the responses of Chinese hamster ovary (CHO) mammalian cells to different temperature shocks. The experimental data show that the mean value response of a cell population can be described by a mathematical expression (empirical law) which is valid for a large range of heat shock conditions. A nonlinear stochastic theoretical model was developed that explains the empirical law for the mean response. Moreover, the theoretical model predicts a specific biological probability distribution of responses for a cell population. The prediction was experimentally confirmed by measurements at the single-cell level. The computational approach can be used to study other nonlinear stochastic biological phenomena.
authors.affiliation: Department of Physics, University of Richmond, Richmond, Virginia, USA. olipan@richmond.edu
authors.forename: Ovidiu
authors.initials: O
authors.lastname: Lipan
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Navenot
authors.forename: Zixuan
authors.initials: Z
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Peiper
mesh_terms: D000818:Animals; D016466:CHO Cells; D003198:Computer Simulation; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000032:analysis; D018869:Heat-Shock Response; D008954:Models, Biological; D017711:Nonlinear Dynamics; D009716:Numerical Analysis, Computer-Assisted; D013269:Stochastic Processes
chemical_list: D006360:Heat-Shock Proteins