title: Isolation of genes expressed during the developmental stages of the oyster mushroom, Pleurotus ostreatus, using expressed sequence tags.
abstract: In order to isolate and identify the developmentally regulated genes during fruiting body development, cDNA libraries were constructed from eight developmental stages of the Oyster mushroom, Pleurotus ostreatus. From these libraries, 11 761 expressed sequence tags (PoESTs) were generated. Of these, 4060 different genes (PoUnigenes) were identified, representing 34.5% of the entire genome. Redundancy analysis of ESTs during the developmental stages identified eight, 13 and two genes that were specifically expressed in mycelia, fruiting body and basidiospore, respectively. RT-PCR was used to confirm the specific expression of nine genes which showed specific redundancy in fruiting body stages, four genes of which were expressed specifically in fruiting body stages as expected in redundancy analysis, and other genes were expressed abundantly in fruiting body stages. The EST database of P. ostreatus generated during this study provides a genetic and biochemical basis for future studies of the developmental stages of basidiomycetes.
authors.affiliation: Department of Applied Biochemistry, College of Natural Science, Konkuk University, Chung-Ju, Korea.
authors.forename: Joong-Ho
authors.initials: JH
authors.lastname: Joh
authors.forename: Seung-Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Jin-sung
authors.initials: JS
authors.lastname: Lee
authors.forename: Ki-Hwan
authors.initials: KH
authors.lastname: Kim
authors.forename: Sung-Ja
authors.initials: SJ
authors.lastname: Jeong
authors.forename: Woong-Han
authors.initials: WH
authors.lastname: Youn
authors.forename: Nam-Kuk
authors.initials: NK
authors.lastname: Kim
authors.forename: Eun-Suk
authors.initials: ES
authors.lastname: Son
authors.forename: Yong-Sup
authors.initials: YS
authors.lastname: Cho
authors.forename: Young-Bok
authors.initials: YB
authors.lastname: Yoo
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Lee
authors.forename: Beom-Gi
authors.initials: BG
authors.lastname: Kim
mesh_terms: D004271:DNA, Fungal / Q000235:genetics; D020224:Expressed Sequence Tags; D048690:Fruiting Bodies, Fungal / Q000235:genetics / Q000254:growth & development; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D005800:Genes, Fungal; D025282:Mycelium / Q000235:genetics / Q000254:growth & development; D020076:Pleurotus / Q000235:genetics* / Q000254:growth & development*; D012331:RNA, Fungal / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development
chemical_list: D004271:DNA, Fungal; D012331:RNA, Fungal; D012333:RNA, Messenger