title: Assessment of soy aeroallergen levels in different work environments.
abstract: BACKGROUND
  Airborne soybean hull proteins are known causes of asthma epidemics around harbours and soy processing plants. Soy flour dust proteins may cause occupational allergy in food and feed industries.
  
  
  OBJECTIVE
  To compare enzyme immunoassays (EIAs) for soy hull and soy flour aeroallergens, exposure assessment in various work environments.
  
  
  METHODS
  Airborne dust samples (n=324) from soy unloading and/or processing plants, the animal feed industry and pig stables were analysed by two soy flour assays: one assay for measuring complete soy hull proteins and two assays for measuring the purified low-molecular-weight (LMW) soy hull allergens.
  
  
  RESULTS
  Immunoblotting confirmed strong differences between antibody specificities and soy preparations. The results of the two soy flour assays and the assay for measuring complete soy hull proteins were highly correlated (r>0.85). The two LMW soy hull assays also showed a strong mutual correlation (r=0.91), but much less correlation with assays for measuring soy flour and complete soy hull. The levels of LMW soy hull proteins were the highest at sites of soybean unloading or processing, while soy flour levels were particularly high in the soy and animal feed industry.
  
  
  CONCLUSIONS
  The optimal EIA procedure for soy aeroallergen exposure assessment depends on the type of work environment and the local soy dust composition. Thus, the type of work environment should always be taken into account in future soy allergy studies in order to prevent a possible underestimation of the workers' actual risk of developing soy allergy.
authors.affiliation: Pulmonology Department, Hospital Universitario Vall d'Hebron, Barcelona, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Gómez-Ollés
authors.forename: M J
authors.initials: MJ
authors.lastname: Cruz
authors.forename: J
authors.initials: J
authors.lastname: Bogdanovic
authors.forename: I M
authors.initials: IM
authors.lastname: Wouters
authors.forename: G
authors.initials: G
authors.lastname: Doekes
authors.forename: I
authors.initials: I
authors.lastname: Sander
authors.forename: F
authors.initials: F
authors.lastname: Morell
authors.forename: M J
authors.initials: MJ
authors.lastname: Rodrigo
mesh_terms: D000393:Air Pollutants / Q000032:analysis* / Q000276:immunology; D000485:Allergens / Q000032:analysis* / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology; D006801:Humans; D007118:Immunoassay; D016273:Occupational Exposure / Q000032:analysis*; D030262:Soybean Proteins / Q000032:analysis* / Q000276:immunology*; D017132:Workplace
chemical_list: D000393:Air Pollutants; D000485:Allergens; D000906:Antibodies; D030262:Soybean Proteins