Preparation and evaluation of rigid porous polyacrylamide-based strong cation-exchange monolithic columns for capillary electrochromatography.
A CEC monolithic column with strong cation-exchange (SCX) stationary phase based on hydrophilic monomers was prepared by in situ polymerization of acrylamide, methylenebisacrylamide, and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) in a complete organic binary porogenic solvent consisting of DMSO and dodecanol. The sulfonic groups provided by the monomer AMPS on the surface of the stationary phase generate an EOF from anode to cathode, and serve as an SCX stationary phase at the same time. The monolithic stationary phase exhibited normal-phase chromatographic behavior for neutral analytes. For charged analytes, electrostatic interaction/repulsion with the monolith was observed. The strong SCX monolithic column has been successfully employed in the electrochromatographic separation of basic drugs, peptides, and alkaloids extracted from natural products.
Dong
Jing
J
National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Ou
Junjie
J
Dong
Xiaoli
X
Wu
Renan
R
Ye
Mingliang
M
Zou
Hanfa
H
D000180:Acrylic Resins / Q000737:chemistry*; D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods*; D002412:Cations / Q000737:chemistry; D007474:Ion Exchange; D010316:Particle Size; D010455:Peptides / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000032:analysis*; D016062:Porosity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties; D013997:Time Factors; D000099166:Polyacrylamides
D000180:Acrylic Resins; D002412:Cations; D010455:Peptides; D004364:Pharmaceutical Preparations; D000099166:Polyacrylamides