Exposure study on chemosensory effects of epsilon-caprolactam in the low concentration range.
OBJECTIVE
Aim of the study was to examine possible chemosensory effects of epsilon-caprolactam in the low concentration range relevant to indoor environmental conditions.


METHODS
Twenty healthy subjects (10 male, 10 female) aged from 21 to 38 years were exposed for 6 h, respectively, to 0, 0.15, 0.5 and 5 mg/m3 epsilon-caprolactam vapours in a randomized and double-blind method. As a measure of trigeminal stimulation of the eye, blink frequency was video-recorded four times per day and evaluated by using a new semi-automatic, computer-assisted method compared to baseline recording and manual counting. Digital slit lamp photographs were taken at the same time to examine conjunctival hyperaemia. A standardized ophthalmologic grading scale was used to measure redness of the eyes objectively. Active anterior rhinomanometry compared nasal resistance before and after exposure. Subjective ratings of discomfort and mental orientation were assessed using the German version of the Swedish Performance Evaluation system (SPES). As a measure of personality traits, positive and negative affectivity was determined (PANAS).


RESULTS
Six hour exposures to epsilon-caprolactam revealed no significant dose-response relationship concerning blink frequency, nasal resistance and redness of the bulbar conjunctiva. Subjective ratings of discomfort (sum scores) significantly increased only at the highest concentration of 5 mg/m3. However, the increase in discomfort was only moderate, ranging between "not at all" and "somewhat". Significant increases of the subjective detection of malodour (subscore) already occurred at 0.15 mg/m3, showing no adaptation over time. Irritation of the eyes or upper airways was not reported.


CONCLUSIONS
Exposure to epsilon-caprolactam vapour did not elicit any acute health effects in a concentration range up to 0.5 mg/m3. Even at the highest concentration of 5 mg/m3, we could only find a slight increase in subjective symptoms, mainly due to an unincisive increase of perception of malodour.
Ziegler
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Institute and Outpatient Clinic for Occupational and Social Medicine, University Hospital of Heidelberg, Vossstrasse 2, 69115 Heidelberg, Germany. budandi3@hotmail.com
Zimmer
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Triebig
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D000328:Adult; D000393:Air Pollutants / Q000009:adverse effects; D001767:Blinking / Q000187:drug effects; D002209:Caprolactam / Q000633:toxicity*; D004311:Double-Blind Method; D005123:Eye / Q000187:drug effects; D005260:Female; D006801:Humans; D019570:Inhalation Exposure; D007509:Irritants / Q000633:toxicity*; D008297:Male; D015508:Nasal Obstruction / Q000139:chemically induced; D009299:Nasal Provocation Tests; D011930:Reaction Time / Q000187:drug effects
D000393:Air Pollutants; D007509:Irritants; D002209:Caprolactam