[Identification of a novel HLA allele B 4446 by sequencing-based typing].
OBJECTIVE
To identify a novel HLA allele in Chinese population.


METHODS
A new HLA-B allele was initially detected by an usual PCR-SSP and PCR-SSOP in routine typing HLA allele. Sequence-based typing (SBT) was used to identify and analysis the difference between the new allele and HLA-B 4409 allele.


RESULTS
The HLA-B exon 3 nucleotide sequence of the novel allele was different from all other known alleles. The allele had 3 nucleotides replaced of the closest matching B 4409 allele at nt538(G>C), nt539(A>T) and nt540 (C>G) in exon 3, resulting in an amino acid change from D(GAC) to L(CTG) at codon 180.


CONCLUSIONS
A novel HLA allele was confirmed by the SBT and it was officially designated as HLA-B 4446 by WHO Nomenclature Committee for Factors of the HLA System in September,2005.
Li
Jian-ping
JP
Liaoning Blood Center, Shenyang, Liaoning, 110044 P. R. China. 123@syxz.com.
Zhang
Xu
X
Li
Xiao-feng
XF
Qu
Zhe
Z
Gao
Jing-bo
JB
Huang
Xu-ying
XY
Liu
Xian-zhi
XZ
D000483:Alleles; D001483:Base Sequence; D015235:HLA-B Antigens / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D015235:HLA-B Antigens