title: Disruption of mouse poly(A) polymerase mGLD-2 does not alter polyadenylation status in oocytes and somatic cells.
abstract: The elongation of poly(A) tails in cytoplasm is essential for oogenesis and early embryogenesis in Xenopus laevis. mGLD-2 is a mouse homologue of Xenopus cytoplasmic poly(A) polymerase xGLD-2. We found an association of mGLD-2 with cytoplasmic polyadenylation components, CPEB and CPSF described in Xenopus oocytes. To clarify the role of mGLD-2 in mouse, we produced an mGLD-2 disrupted mouse line by homologous recombination. In spite of the ubiquitous expression of mGLD-2, the disrupted mice were apparently normal and healthy. Moreover, it was demonstrated that mGLD-2 disruption did not affect the poly(A) tail elongation in oocytes using reporter RNAs. Coincide with these observations, the maturation of the oocytes was normal and the mice were fertile. Thus mGLD-2 is dispensable for full-term development and oogenesis. Our results also indicate that there is another source of cytoplasmic poly(A) polymerase in mouse.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan. tomo@grape.med.tottori-u.ac.jp
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kumagai
authors.forename: Masanori
authors.initials: M
authors.lastname: Kimura
authors.forename: Hiromi
authors.initials: H
authors.lastname: Watanabe
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sakurai
authors.forename: Minoru
authors.initials: M
authors.lastname: Kimura
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Kashiwabara
authors.forename: Tadashi
authors.initials: T
authors.lastname: Baba
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D008099:Liver / Q000378:metabolism*; D051379:Mice; D009865:Oocytes / Q000378:metabolism*; D026723:Polyadenylation / Q000187:drug effects*; D011062:Polynucleotide Adenylyltransferase / Q000235:genetics* / Q000378:metabolism
chemical_list: D011062:Polynucleotide Adenylyltransferase