PCR amplification of microsatellites from single cells of Karenia brevis preserved in Lugol's iodine solution.
A simple and effective protocol is described for multiplex polymerase chain reaction (PCR) amplification of single cells of Karenia brevis. The protocol requires minimum processing, avoids additions that might dilute target DNA template, and can be used on cells preserved in Lugol's iodine preservative. Destaining of Lugol's-preserved cells with sodium thiosulfate allowed successful amplification of single-copy, nuclear-encoded microsatellites in single cells of K. brevis that have been preserved for up to 6 years.
Henrichs
D W
DW
Department of Biology, Texas A and M University, College Station, Texas 77843-3578, USA. dhenrichs@bio.tamu.edu
Renshaw
M A
MA
Santamaria
C A
CA
Richardson
B
B
Gold
J R
JR
Campbell
L
L
D000483:Alleles; D000818:Animals; D004141:Dinoflagellida / Q000235:genetics*; D005838:Genotype; D007454:Iodides; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D011309:Preservation, Biological / Q000379:methods
D007454:Iodides; C010389:Lugol's solution