title: Vertebral endplate trauma induces disc cell apoptosis and promotes organ degeneration in vitro.
abstract: There is a major controversy whether spinal trauma with vertebral endplate fractures can result in post-traumatic disc degeneration. Intervertebral discs, which are adjacent to burst endplates, are frequently removed and an intercorporal spondylodesis is performed. In any case, the biological effects within the discs following endplate fractures are poorly elucidated to date. The aim of our investigations was therefore to establish a novel disc/endplate trauma culture model to reproducibly induce endplate fractures and investigate concurrent disc changes in vitro. This model is based on a full-organ disc/endplate culture system, which has been validated by the authors before. Intervertebral disc/endplate specimens were isolated from Burgundy rabbits and cultured in standard media (DMEM/F12, 10%FCS). Burst endplate fractures were induced in half of the specimens with a custom-made fracture device and subsequently cultured for 9 days. The biological effects such as necrotic or apoptotic cell death and the expression of pro-apoptotic genes and other genes involved in organ degeneration, e.g. matrix metalloproteinases (MMPs) were analyzed. Cell damage was assessed by quantification of the lactate dehydrogenase (LDH) activity in the supernatant. The expression of genes involved in the cellular apoptotic pathway (caspase 3) and the pro-apoptotic proteins FasL and TNF-alpha were monitored. The results demonstrate that LDH levels increased significantly post trauma compared to the control and remained elevated for 3 days. Furthermore, a constant up-regulation of the caspase 3 gene in both disc compartments was present. The pro-apoptotic proteins FasL and TNF-alpha were up regulated predominantly in the nucleus whereas the MMP-1 and -13 transcripts (collagenases) were increased in both disc structures. From this study we can conclude that endplate burst fractures result in both necrotic and apoptotic cell death in nucleus and annulus tissue. Moreover, FasL and TNF-alpha expression by nucleus cells may lead to continued apoptosis induced by Fas- and TNF-alpha receptor bearing cells. In addition TNF-alpha over-expression has potentially deleterious effects on disc metabolism such as over-expression of matrix proteinases. Taken together, the short term biological response of the disc following endplate fracture exhibits characteristics, which may initiate the degeneration of the organ.
authors.affiliation: MEM Research Center for Orthopaedic Surgery, Institute for Surgical Technology and Biomechanics, University of Bern, Stauffacherstrasse 78, 3014, Bern, Switzerland. Daniel.Haschtmann@MEMcenter.unibe.ch
authors.forename: Daniel
authors.initials: D
authors.lastname: Haschtmann
authors.forename: Jivko V
authors.initials: JV
authors.lastname: Stoyanov
authors.forename: Philippe
authors.initials: P
authors.lastname: Gédet
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Ferguson
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000502:physiology; D004195:Disease Models, Animal; D053222:Fas Ligand Protein / Q000378:metabolism; D007403:Intervertebral Disc / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008159:Lumbar Vertebrae / Q000293:injuries*; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D009924:Organ Culture Techniques; D011817:Rabbits; D013122:Spinal Diseases / Q000209:etiology* / Q000378:metabolism / Q000473:pathology*; D016103:Spinal Fractures / Q000150:complications* / Q000503:physiopathology; D013904:Thoracic Vertebrae / Q000293:injuries*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053222:Fas Ligand Protein; D014409:Tumor Necrosis Factor-alpha; D007770:L-Lactate Dehydrogenase; D053148:Caspase 3; D053509:Matrix Metalloproteinase 13; D020781:Matrix Metalloproteinase 1