title: In vitro cultured peripheral blood mononuclear cells from patients with chronic schistosomiasis mansoni show immunomodulation of cyclin D1,2,3 in the presence of soluble egg antigens.
abstract: Infection with Schistosoma mansoni induces a wide range of effects on the immune responses of the host. In the present study we investigated the influence of soluble egg antigens (SEA) on the cell cycle of peripheral blood mononuclear cells (PBMC) from infected and non-infected individuals with S. mansoni resident in an endemic area and blood donors from non-endemic area. The cell cycle, the expression of activation markers and cyclin D(+)(1,2,3) CD3(+) frequency was assessed by flow cytometry. Stimulation of PBMC from infected patients with SEA resulted in a lower frequency of CD3(+) T cells in S phase when compared with the non-infected group. In addition, infected patients presented a decrease of activation marker expression (CD69(+), HLA-DR(+) and CD28(-) on CD4(+) cells and CD25(+), HLA-DR(+) on CD8(+) cells). A reduced frequency was observed of cyclin D(1,2,3) expression in SEA-stimulated T cells from infected individuals when compared with those from the non-infected group. The decreased expression of activation markers and frequency of cyclin D(1,2,3) in T cells may result in arrest of T cells in the G(0)/G(1) phase of the cell cycle, thus explaining the down-regulation observed in chronic schistosomiasis.
authors.affiliation: Laboratório de Imunologia Celular e Molecular, Instituto René Rachou/FIOCRUZ, Fundação Oswaldo Cruz, Avenida Augusto de Lima 1715, Barro Preto, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Campi-Azevedo
authors.forename: Giovanni
authors.initials: G
authors.lastname: Gazzinelli
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Bottazzi
authors.forename: Andréa
authors.initials: A
authors.lastname: Teixeira-Carvalho
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Corrêa-Oliveira
authors.forename: Iramaya Rodrigues
authors.initials: IR
authors.lastname: Caldas
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D017252:CD3 Complex / Q000378:metabolism; D002478:Cells, Cultured; D002908:Chronic Disease; D019938:Cyclin D1 / Q000378:metabolism*; D056742:Cyclin D2; D056743:Cyclin D3; D016213:Cyclins / Q000378:metabolism*; D015536:Down-Regulation; D005243:Feces / Q000469:parasitology; D016193:G1 Phase / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D010270:Parasite Egg Count; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000469:parasitology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; C080336:CCND2 protein, human; C534696:CCND3 protein, human; D017252:CD3 Complex; D056742:Cyclin D2; D056743:Cyclin D3; D016213:Cyclins; D019938:Cyclin D1