title: Leishmaniasis in central and southern Tunisia: current geographical distribution of zymodemes.
abstract: The authors report the identification of Leishmania strains isolated from the Centre and the South of Tunisia. 266 strains were isolated between 1998 and 2006 from human (n=221 strains) and dogs (n=45 strains) hosts. The isoenzymatic identification exhibits the presence of in total five zymodemes belonging to three Leishmanio complexes: Leishmania infantum, L. major and L. killicki. All strains isolated from human and canine visceral leishmaniasis belonged to L. infantum. zymodeme MON-1 was the only one isolated from canine visceral leishmaniasis. However, it is predominant in human visceral leishmaniasis beside zymodeme MON-24 which was detected in two provinces of the Centre (Monastir and Kairouan) and zymodeme MON-80 isolated for the first time in Kairouan province. Three complexes are responsible for human cutaneous leishmaniasis: L. major MON-25 is the parasite the most frequently found in its classic foci in the Centre and the South of the country. L. infantum MON-24 was isolated for the first time in a small locality of Sfax (southern Tunisia) showing the appearance of a new focus of L. infantum. L. killicki was isolated in its original focus of Tataouine and in two new foci of the central part of the country (Sidi Bouzid and Kairouan).
authors.affiliation: Laboratoire de Parasitologie-Mycologie (99UR/08-05), Faculté de Pharmacie, Département de Biologie, Clinique B, 1 Rue Avicenne, 5000 Monastir, Tunisia.
authors.forename: N
authors.initials: N
authors.lastname: Haouas
authors.forename: M
authors.initials: M
authors.lastname: Gorcii
authors.forename: N
authors.initials: N
authors.lastname: Chargui
authors.forename: K
authors.initials: K
authors.lastname: Aoun
authors.forename: A
authors.initials: A
authors.lastname: Bouratbine
authors.forename: F
authors.initials: F
authors.lastname: Messaadi Akrout
authors.forename: A
authors.initials: A
authors.lastname: Masmoudi
authors.forename: J
authors.initials: J
authors.lastname: Zili
authors.forename: M
authors.initials: M
authors.lastname: Ben Said
authors.forename: F
authors.initials: F
authors.lastname: Pratlong
authors.forename: J P
authors.initials: JP
authors.lastname: Dedet
authors.forename: H
authors.initials: H
authors.lastname: Mezhoud
authors.forename: R
authors.initials: R
authors.lastname: Azaiez
authors.forename: H
authors.initials: H
authors.lastname: Babba
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000453:epidemiology* / Q000635:transmission; D004285:Dogs; D006801:Humans; D007891:Leishmania / Q000302:isolation & purification*; D018314:Leishmania infantum / Q000302:isolation & purification; D018320:Leishmania major / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000453:epidemiology* / Q000635:transmission / Q000662:veterinary; D007898:Leishmaniasis, Visceral / Q000453:epidemiology* / Q000635:transmission / Q000662:veterinary; D014416:Tunisia / Q000453:epidemiology; D015047:Zoonoses