Investigating translation initiation using Drosophila molecular genetics.
Genetic tools enable insights into how translation controls development of a multicellular organism. Different genetic approaches offer the ability to manipulate the Drosophila genome in very precise ways, thereby allowing the investigation of how translation factors work in the context of a whole organism. We present here an overview of selected techniques used to identify genes involved in translation initiation, and quantitative methods to characterize phenotypes caused by mutations in genes encoding translation initiation or regulatory factors.
Tettweiler
Gritta
G
Department of Biology and DBRI, McGill University, Montreal, Quebec, Canada.
Lasko
Paul
P
D000818:Animals; D004254:DNA Nucleotidyltransferases / Q000502:physiology; D004251:DNA Transposable Elements / Q000502:physiology; D004331:Drosophila melanogaster / Q000235:genetics*; D010442:Peptide Chain Initiation, Translational / Q000502:physiology*; D034622:RNA Interference; D011995:Recombination, Genetic; D014157:Transcription Factors / Q000502:physiology
D004251:DNA Transposable Elements; D014157:Transcription Factors; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase