Structural determination of a neutral exopolysaccharide produced by Lactobacillus delbrueckii ssp. bulgaricus LBB.B332.
The neutral exopolysaccharide produced by Lactobacillus delbrueckii ssp. bulgaricus LBB.B332 in skimmed milk was found to be composed of d-glucose, d-galactose, and l-rhamnose in a molar ratio of 1:2:2. Linkage analysis and 1D/2D NMR (1H and 13C) studies carried out on the native polysaccharide as well as on an oligosaccharide generated by a periodate oxidation protocol, showed the polysaccharide to consist of linear pentasaccharide repeating units with the following structure: -->3-alpha-D-Glcp-(1-->3)-alpha-D-Galp-(1-->3)-alpha-L-Rhap-(1-->2)-alpha-L-Rhap-(1-->2)-alpha-D-Galp-(1-->.
Sánchez-Medina
Inmaculada
I
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Padualaan 8, NL-3584 CH Utrecht, The Netherlands.
Gerwig
Gerrit J
GJ
Urshev
Zoltan L
ZL
Kamerling
Johannis P
JP
D000818:Animals; D002240:Carbohydrate Sequence; D048668:Lactobacillus delbrueckii / Q000145:classification / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008892:Milk / Q000382:microbiology; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000737:chemistry; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D014312:Trisaccharides / Q000032:analysis / Q000737:chemistry
D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D014312:Trisaccharides; D010504:Periodic Acid; C009288:metaperiodate