Dissociation of spinal microglia morphological activation and peripheral inflammation in inflammatory pain models.
We compared the effects of peripheral Freund's Complete Adjuvant (CFA) and formalin injection on spinal microglia activation. Both qualitative and quantitative analyses showed signs of microglia activation on the ipsilateral side of the lumbar dorsal horn on day 3, day 7 and day 14 after formalin injection. However, significant microglia morphological alteration was not found in the CFA model. At the injection site in the paw, CFA injection induced considerably more inflammation than formalin injection. Although spinal microglia might be activated in inflammatory pain models, morphologically, spinal microglia activation was not closely correlated with peripheral inflammation.
Lin
Ting
T
Center for TMD & Orofacial Pain, Peking University School & Hospital of Stomatology, Beijing 100081, PR China.
Li
Kai
K
Zhang
Fei-Yu
FY
Zhang
Zhen-Kang
ZK
Light
Alan R
AR
Fu
Kai-Yuan
KY
D000818:Animals; D001522:Behavior, Animal; D039481:CD11b Antigen / Q000378:metabolism; D002452:Cell Count; D051545:Cyclooxygenase 1 / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004195:Disease Models, Animal; D005404:Fixatives / Q000009:adverse effects; D005557:Formaldehyde / Q000009:adverse effects; D005620:Freund's Adjuvant; D006930:Hyperalgesia / Q000209:etiology / Q000503:physiopathology; D007249:Inflammation / Q000139:chemically induced / Q000150:complications*; D008297:Male; D017628:Microglia / Q000187:drug effects / Q000473:pathology*; D010146:Pain / Q000209:etiology* / Q000473:pathology*; D010147:Pain Measurement; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects; D013116:Spinal Cord / Q000473:pathology; D013997:Time Factors
D039481:CD11b Antigen; D005404:Fixatives; D005557:Formaldehyde; D005620:Freund's Adjuvant; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2