title: Assessment of prion inactivation by combined use of Bacillus-derived protease and SDS.
abstract: Prions, infectious agents causing transmissible spongiform encephalopathy, retain infectivity even after undergoing routine sterilization processes. We found that MSK103 protease, identified in our previous study, effectively reduces infectivity and the level of misfolded isoform of the prion protein in scrapie-infected brain homogenates in the presence of SDS. The treatment therefore can be applied to the decontamination of thermolabile instruments.
authors.affiliation: Prion Disease Research Center, National Institute of Animal Health, Japan.
authors.forename: Miyako
authors.initials: M
authors.lastname: Yoshioka
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Murayama
authors.forename: Takehiro
authors.initials: T
authors.lastname: Miwa
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Miura
authors.forename: Masuhiro
authors.initials: M
authors.lastname: Takata
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Koji
authors.initials: K
authors.lastname: Nishizawa
authors.forename: Shirou
authors.initials: S
authors.lastname: Mohri
mesh_terms: D000818:Animals; D001407:Bacillus / Q000201:enzymology*; D004357:Drug Synergism; D006863:Hydrogen-Ion Concentration; D007276:Injections, Intraventricular; D051379:Mice; D008822:Mice, Transgenic; D010447:Peptide Hydrolases / Q000302:isolation & purification / Q000494:pharmacology*; D018620:PrPSc Proteins / Q000008:administration & dosage / Q000378:metabolism*; D011489:Protein Denaturation; D020033:Protein Isoforms; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D013242:Sterilization / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D018620:PrPSc Proteins; D020033:Protein Isoforms; D012967:Sodium Dodecyl Sulfate; D010447:Peptide Hydrolases