Anion-exchange chromatography-electrospray ionization mass spectrometry method development for the environmental analysis of aliphatic polyhydroxy carboxylic acids.
For environmental analytical purposes, an anion-exchange chromatography-electrospray ionization MS method was developed enabling the identification and quantification of 18 aliphatic (poly)hydroxy (poly)carboxylic acids including sugar and uronic acids. The initially incomplete separation could be partially improved for specific analyte combinations adding borate, the zwitter ionic MES, or an alkylglycoside to the NaOH gradient supplied with the Dionex IonPac AS-11 HC column. Despite the incomplete separation, reproducible and sensitive identification and quantification is possible with MS detection in the selected ion-monitoring mode. Applying an internal standard for the compensation of the sensitivity drift of the detector, linear calibration functions were calculated for all analytes. Limits of detection spanned between 18 and 71 microgL(-1) which could be further improved for some more hydrophobic analytes by post-column addition of MeOH.
Meyer
Axel
A
Department of Analytical and Ecological Chemistry, Faculty VI - Geography/Geosciences, University of Trier, Campus II, Behringstrasse 21, D-54296 Trier, Germany.
Höffler
Susanne
S
Fischer
Klaus
K
D000838:Anions; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry; D002264:Carboxylic Acids / Q000032:analysis* / Q000737:chemistry; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D006878:Hydroxides; D017680:Potassium Compounds; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014574:Uronic Acids / Q000032:analysis / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*
D000838:Anions; D002241:Carbohydrates; D002264:Carboxylic Acids; D005227:Fatty Acids; D006878:Hydroxides; D017680:Potassium Compounds; D012997:Solvents; D014574:Uronic Acids; D014874:Water Pollutants, Chemical; C029943:potassium hydroxide