title: Production of antibodies for selective detection of malachite green and the related triphenylmethane dyes in fish and fishpond water.
abstract: This study provides a practical method for production of the antibodies against malachite green (MG) and its primary metabolite leucomalachite green (LMG). Two ELISA kits are constructed with the MG and LMG antibodies for detection of the residual MG and LMG in fish muscle and fishpond water. The detection limit is established at the level of 0.05 microg/L for both MG and LMG. Our ELISA kits show the advantages of good specificity, high sensitivity, and convenience in rapid screening of MG and LMG residues. The sample of fishpond water, without extraction or prior preparation, is directly assayed by the ELISA kit. More then 80 fish samples can be simultaneously tested in a kit. The toxic crystal violet and its metabolite leucocrystal violet of illegal use in aquaculture are detected by our prepared MG and LMG antibodies, whereas the antibodies do not cross-react with common antibiotics, sulfonamides, and benzene derivatives.
authors.affiliation: GlycoNex Inc., Taipei County, 221, Taiwan.
authors.forename: Mei-Chun
authors.initials: MC
authors.lastname: Yang
authors.forename: Jim-Min
authors.initials: JM
authors.lastname: Fang
authors.forename: Tzong-Fu
authors.initials: TF
authors.lastname: Kuo
authors.forename: Da-Ming
authors.initials: DM
authors.lastname: Wang
authors.forename: Yi-Lin
authors.initials: YL
authors.lastname: Huang
authors.forename: Liang-Yirn
authors.initials: LY
authors.lastname: Liu
authors.forename: Pen-Heng
authors.initials: PH
authors.lastname: Chen
authors.forename: Tong-Hsuan
authors.initials: TH
authors.lastname: Chang
mesh_terms: D000814:Aniline Compounds / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D017756:Aquaculture; D004797:Enzyme-Linked Immunosorbent Assay; D005399:Fishes; D005659:Fungicides, Industrial / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D012394:Rosaniline Dyes / Q000032:analysis* / Q000276:immunology; D014867:Water / Q000032:analysis*
chemical_list: D000814:Aniline Compounds; D000906:Antibodies; D005659:Fungicides, Industrial; D012394:Rosaniline Dyes; D014867:Water; C005095:malachite green; C015320:leucomalachite green