title: Crystal structure of the MarR family regulatory protein, ST1710, from Sulfolobus tokodaii strain 7.
abstract: The emergence of bacterial resistance to multiple drugs poses a serious and growing health concern. Understanding and deciphering the mechanisms of these multiple drug resistance regulatory proteins through structural or biochemical means is an important endeavor. Here, we present the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 in two different crystal forms, at 1.80 and 2.0A, respectively. The overall structure of the ST1710 dimer shares the topology of the MarR family of proteins, with each subunit containing a winged helix-turn-helix DNA-binding motif. We also show the protein-DNA interactions by biochemical methods. Our molecular modeling analysis suggested that Asp88 and Arg90 are the key residues in ST1710 involved in the protein-DNA interactions.
authors.affiliation: RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan. tskvel@spring8.or.jp
authors.forename: Thirumananseri
authors.initials: T
authors.lastname: Kumarevel
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Megumi
authors.initials: M
authors.lastname: Nishio
authors.forename: Subash C B
authors.initials: SC
authors.lastname: Gopinath
authors.forename: Koji
authors.initials: K
authors.lastname: Takio
authors.forename: Akeo
authors.initials: A
authors.lastname: Shinkai
authors.forename: Penmetcha K R
authors.initials: PK
authors.lastname: Kumar
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry* / Q000378:metabolism*; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D019077:Helix-Turn-Helix Motifs; D008969:Molecular Sequence Data; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship; D016944:Sulfolobus / Q000378:metabolism*
chemical_list: D019843:Archaeal Proteins; D004268:DNA-Binding Proteins