title: Immunization with radioattenuated yeast cells of Paracoccidioides brasiliensis induces a long lasting protection in BALB/c mice.
abstract: Paracoccidioides brasiliensis is the fungus agent of paracoccidioidomycosis, a chronic systemic disease prevalent in Latin America. The aim of the present work was to evaluate the protection elicited by the immunization of BALB/c mice with radioattenuated yeast cells of P. brasiliensis. The immunization promoted a long lasting protection against highly infective yeast forms of P. brasiliensis. A 99.5% decrease in CFUs recovery was verified 90 days post challenge. At the same time the levels of IgG2a and IFN-gamma were high while a very low production of IL-10 and IL-5 was verified, suggesting that a Th1 pattern was dominant. This work shows the potential of radioattenuated yeast cells for the development of vaccines against fungi infections.
authors.affiliation: Laboratório de Radiobiologia, Centro de Desenvolvimento da Tecnologia Nuclear, Caixa postal 941, CEP 30123-970, Belo Horizonte, MG, Brazil. estefaniabio@yahoo.com.br <estefaniabio@yahoo.com.br>
authors.forename: Estefânia Mara
authors.initials: EM
authors.lastname: do Nascimento Martins
authors.forename: Bernardo Sgarbi
authors.initials: BS
authors.lastname: Reis
authors.forename: Viviane Cristina
authors.initials: VC
authors.lastname: Fernandes
authors.forename: Míriam Maria Silva
authors.initials: MM
authors.lastname: Costa
authors.forename: Alfredo Miranda
authors.initials: AM
authors.lastname: Goes
authors.forename: Antero Silva Ribeiro
authors.initials: AS
authors.lastname: de Andrade
mesh_terms: D000818:Animals; D005657:Fungal Vaccines / Q000276:immunology* / Q000494:pharmacology; D005720:Gamma Rays; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology; D015848:Interleukin-5 / Q000276:immunology; D007843:Latin America; D051379:Mice; D008807:Mice, Inbred BALB C; D010228:Paracoccidioides / Q000276:immunology* / Q000472:pathogenicity; D010229:Paracoccidioidomycosis / Q000276:immunology / Q000517:prevention & control*; D018417:Th1 Cells / Q000276:immunology; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000276:immunology / Q000494:pharmacology
chemical_list: D005657:Fungal Vaccines; D007074:Immunoglobulin G; D015848:Interleukin-5; D014613:Vaccines, Attenuated; D016753:Interleukin-10; D007371:Interferon-gamma