title: A Yersinia pestis lpxM-mutant live vaccine induces enhanced immunity against bubonic plague in mice and guinea pigs.
abstract: The lpxM mutant of the live vaccine Yersinia pestis EV NIIEG strain synthesising a less toxic penta-acylated lipopolysaccharide was found to be avirulent in mice and guinea pigs, notably showing no measurable virulence in Balb/c mice which do retain some susceptibility to the parental strain itself. Twenty-one days after a single injection of the lpxM-mutant, 85-100% protection was achieved in outbred mice and guinea pigs, whereas a 43% protection rate was achieved in Balb/c mice given single low doses (10(3) to 2.5 x 10(4) CFU) of this vaccine. A subcutaneous challenge with 2000 median lethal doses (equal to 20,000 CFU) of fully virulent Y. pestis 231 strain, is a 6-10-fold higher dose than that which the EV NIIEG itself can protect against.
authors.affiliation: Russian State Anti-Plague Research Institute Microbe, 46 Universitetskaya Street, Saratov 410005, Russia. feodorova@san.ru
authors.forename: V A
authors.initials: VA
authors.lastname: Feodorova
authors.forename: L N
authors.initials: LN
authors.lastname: Pan'kina
authors.forename: E P
authors.initials: EP
authors.lastname: Savostina
authors.forename: L V
authors.initials: LV
authors.lastname: Sayapina
authors.forename: V L
authors.initials: VL
authors.lastname: Motin
authors.forename: S V
authors.initials: SV
authors.lastname: Dentovskaya
authors.forename: R Z
authors.initials: RZ
authors.lastname: Shaikhutdinova
authors.forename: S A
authors.initials: SA
authors.lastname: Ivanov
authors.forename: B
authors.initials: B
authors.lastname: Lindner
authors.forename: A N
authors.initials: AN
authors.lastname: Kondakova
authors.forename: O V
authors.initials: OV
authors.lastname: Bystrova
authors.forename: N A
authors.initials: NA
authors.lastname: Kocharova
authors.forename: S N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: O
authors.initials: O
authors.lastname: Holst
authors.forename: G B
authors.initials: GB
authors.lastname: Pier
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
authors.forename: A P
authors.initials: AP
authors.lastname: Anisimov
mesh_terms: D000818:Animals; D005260:Female; D017353:Gene Deletion; D006168:Guinea Pigs; D008050:Lipid A / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010930:Plague / Q000517:prevention & control*; D010931:Plague Vaccine / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000276:immunology; D014774:Virulence; D015010:Yersinia pestis / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D008050:Lipid A; D010931:Plague Vaccine; D014613:Vaccines, Attenuated