An association between parvovirus B19 and Kikuchi-Fujimoto disease.
Although Kikuchi-Fujimoto disease (KFD) has a higher prevalence among Asian countries, it is a well-defined entity throughout the world. However, its etiology and pathogenesis remain undetermined. To study whether B19 infection is associated with idiopathic KFD (iKFD), we examined the presence of the viral genome and proteins in paraffin-embedded tissues of lymph nodes retrospectively from 33 iKFD patients and 16 age- and sex-matched control subjects by nested PCR (nPCR), in situ hybridization (ISH), and immunohistochemistry (IHC). B19 was detected in 87.1, 69.7, and 57.6% of iKFD specimens by nPCR, ISH, and IHC, respectively, whereas the virus was positive in only 56.3, 31.3, and 25.0% of control tissues by the respective methods (nPCR: p = 0.029; ISH: p = 0.011; IHC: p = 0.032). The IHC-ISH double-staining assay demonstrated that B19-infected cells were mainly lymphocytes and a small number of histiocytes. These results showed for the first time a high frequency of localized persistence of B19 in lymph nodes from iKFD patients, suggesting that B19 might play an important role in the pathogenesis of iKFD.
Zhang
Wei-Ping
WP
Department of Pathology and State Key Laboratory of Cancer Biology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, People's Republic of China.
Wang
Juan-Hong
JH
Wang
Wen-Qing
WQ
Chen
Xie-Qun
XQ
Wang
Zhe
Z
Li
Yuan-Fei
YF
Hu
Pei-Zhen
PZ
Zhang
Wei
W
Wang
Lu
L
Wang
Di
D
Huang
Gao-Sheng
GS
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006644:Histiocytes / Q000821:virology; D020042:Histiocytic Necrotizing Lymphadenitis / Q000209:etiology* / Q000821:virology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008198:Lymph Nodes / Q000821:virology; D008214:Lymphocytes / Q000821:virology; D008297:Male; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000821:virology*; D016732:Parvovirus B19, Human; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies
D004279:DNA, Viral