[Application of DNA microcarriers for evaluation of mice splenocytes genes expression after immunization with pertussis vaccines and challenged with different B. pertussis strains].
The study aimed at identification of splenocytes genes expression induced after immunization with whole-cell or acellular pertussis vaccines and subsequent challenge with B. pertussis strains of different ptxS1/prn genes alleles in correlation with differences in bacteria elimination from the lung tissue. Investigations were performed using DNA microcarriers. Analysis of genes expression generated through B. pertussis challenge on DNA microcarriers confirmed the role of specific B. pertussis virulence factors in differences of induced transcription response in the host. Level of differences in splenocytes transcriptional response of mice immunized with whole-cell pertussis vaccine was higher than that found in animals immunized with acellular pertussis vaccine. Level of genes expression change induced with B. pertussis 2316 strain was as much as twice higher than that found after challenge with B. pertussis 629 strain. Levels of gene expression change during challenge with B. pertussis 629 and 2316 strains were similar after immunization with acellular pertussis vaccine. Differences of transcriptional response induced with B. pertussis 2316 strain were associated with induction of immunological response, activation of inflammatory mediators, as well as with synthesis of heat shock proteins. Further studies performed with DNA microcarriers on sufficient number of B. pertussis strains might confirm this initial screening of genes identification involved in activation and repression of basic regulatory pathways after challenge with B. pertussis strains variant's for statistical evaluation of functional differences detected in presented study.
Gzyl
Anna
A
Zakład Badania Surowic i Szczepionek PZH w Warszawie.
Woyciechowska-Oczko
Małgorzata
M
Jarzab
Barbara
B
Augustynowicz
Ewa
E
Mosiej
Ewa
E
Slusarczyk
Janusz
J
D000281:Administration, Intranasal; D000483:Alleles; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology; D001886:Bordetella pertussis / Q000235:genetics* / Q000276:immunology*; D002478:Cells, Cultured; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000276:immunology*; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines / Q000276:immunology*; D005786:Gene Expression Regulation / Q000235:genetics* / Q000276:immunology*; D007114:Immunization / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D037342:Pertussis Toxin / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000502:physiology*; D010566:Virulence Factors, Bordetella / Q000235:genetics / Q000276:immunology; D014917:Whooping Cough / Q000235:genetics / Q000276:immunology / Q000517:prevention & control
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines; D010566:Virulence Factors, Bordetella; C083388:pertussis toxin, S1 subunit; C068929:pertactin; D037342:Pertussis Toxin