Cloning and expression pattern of dog SDF-1 and the implications of altered expression of SDF-1 in ischemic myocardium.
Stromal cell derived factor-1 (SDF-1) plays a pivotal role in the mobilization and homing of stem cells, indicative of its potential in myocardial regeneration after heart damage. The use of large animal models in cardiac surgery research plays an essential role in the translation of results from basic studies into clinical trials. Considering the aforesaid two reasons, for the first time, we cloned dog SDF-1 cDNA and characterized the constitutive expression pattern of SDF-1 gene in normal dog tissues and the dynamic expression pattern in a dog model of myocardial infarction (MI) by means of ELISA test, real-time RT-PCR, and Western blotting. In a dog model of MI, We also examined and compared the differentially expressed pattern of SDF-1 between infarct area and border zone of myocardium in order to explore the implication of differentially distribution of SDF-1 in the mobilization and homing of stem cells to the damaged heart. Our results provide insights into expression pattern and pathophysiologic significance of dog SDF-1 in normal and heart-damaged dogs.
Wei
Ying-jie
YJ
Key Laboratory of Cardiovascular Regenerative Medicine, Ministry of Health, and Department of Cardiovascular Surgery, Cardiovascular Institute and Fu-Wai Hospital, PUMC and CAMS, 167 Beilishilu, Beijing 100037, China.
Tang
Yi
Y
Li
Jun
J
Cui
Chuan-jue
CJ
Zhang
Hong
H
Zhang
Xiao-ling
XL
Zhang
Hao
H
Hu
Sheng-shou
SS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D004195:Disease Models, Animal; D004285:Dogs; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009928:Organ Specificity / Q000235:genetics; D051381:Rats
D054377:Chemokine CXCL12