In vitro exposure of human chondrocytes to pulsed electromagnetic fields.
The effect of pulsed electromagnetic fields (PEMFs) on the proliferation and survival of matrix-induced autologous chondrocyte implantation (MACI)-derived cells was studied to ascertain the healing potential of PEMFs. MACI-derived cells were taken from cartilage biopsies 6 months after surgery and cultured. No dedifferentiation towards the fibro- blastic phenotype occurred, indicating the success of the surgical implantation. The MACI-derived cultured chondrocytes were exposed to 12 h/day (short term) or 4 h/day (long term) PEMFs exposure (magnetic field intensity, 2 mT; frequency, 75 Hz) and proliferation rate determined by flow cytometric analysis. The PEMFs exposure elicited a significant increase of cell number in the SG2M cell cycle phase. Moreover, cells isolated from MACI scaffolds showed the presence of collagen type II, a typical marker of chondrocyte functionality. The results show that MACI membranes represent an optimal bioengineering device to support chondrocyte growth and proliferation in surgical implants. The surgical implant of MACI combined with physiotherapy is suggested as a promising approach for a faster and safer treatment of cartilage traumatic lesions.
Nicolin
Vanessa
V
Department of Biomedicine - Section of Human Morphology, University of Trieste, Trieste, Italy. nicolin@units.it
Ponti
C
C
Baldini
G
G
Gibellini
D
D
Bortul
R
R
Zweyer
M
M
Martinelli
B
B
Narducci
P
P
D002358:Cartilage, Articular / Q000473:pathology* / Q000601:surgery; D049109:Cell Proliferation; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000473:pathology / Q000528:radiation effects* / Q000637:transplantation; D004574:Electromagnetic Fields; D006801:Humans; D007150:Immunohistochemistry; D007718:Knee Injuries / Q000473:pathology* / Q000601:surgery; D007719:Knee Joint / Q000473:pathology* / Q000601:surgery