title: Taenia solium metacestode immunodominant peptides recognized by IgG antibodies in cerebrospinal fluid and serum paired samples from patients with active and inactive neurocysticercosis.
abstract: The aim of this study was to test if serological distinction between patients with active and inactive neurocysticercosis (NCC), could be accomplished by the recognition of immunodominant peptides in total saline antigenic extract of Taenia solium metacestodes by IgG antibody in cerebrospinal fluid (CSF) and serum paired samples. CSF and serum samples of 10 each, active NCC patients, inactive NCC, and individuals with other neurological disorders, were used to recognize the antigenic peptides by western blot (WB). In the active NCC the 28-32 and 39-42 kDa peptides were more frequently detected in CSF than in sera (p < 0.05). The 47-52, 64-68, and 70 kDa antigens showed high frequencies in both samples from patients with active NCC. All the CSF samples of inactive NCC and other neurological disorder (control) patients tested negative, while serum samples from these last two groups recognized mainly the 80, 86, 95, and 98 kDa bands. This finding eliminates the use of the high molecular weigh bands (>or= 80 kDa) for diagnosis of NCC. The final conclusions were that the difference between active and inactive NCC may be done with the detection of peptides only in the CSF samples and that the 47-52, 64-68, and 70 kDa bands may be included as specific markers for active NCC when detected in CSF samples by WB using total saline extract of T. solium metacestode.
authors.affiliation: Laboratório de Parasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, 38400-902 Uberlândia, MG, Brasil.
authors.forename: Ivanildes Solange da Costa
authors.initials: IS
authors.lastname: Barcelos
authors.forename: Leandro Pajuaba de
authors.initials: LP
authors.lastname: Moura
authors.forename: Vinicius Paulino da
authors.initials: VP
authors.lastname: Costa
authors.forename: Marcelo Simão
authors.initials: MS
authors.lastname: Ferreira
authors.forename: Julia Maria
authors.initials: JM
authors.lastname: Costa-Cruz
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000134:cerebrospinal fluid; D015153:Blotting, Western; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology*; D020019:Neurocysticercosis / Q000175:diagnosis*; D010455:Peptides / Q000276:immunology*; D012680:Sensitivity and Specificity; D041201:Taenia solium / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D010455:Peptides