title: Evaluation of platelet and leukocyte functions in effort angina patients using high shear conditions in small-sized collagen bead columns.
abstract: This study used a small-sized collagen bead column system to investigate platelet functions under high shear stress in 28 patients with effort angina and 15 healthy controls. Soluble P-selectin and soluble L-selectin were also evaluated. Patients underwent stent insertion, followed by treatment with aspirin (100 mg/day) and ticlopidine (200 mg/day). High-shear-dependent platelet function was measured using small-sized collagen beads. The retention rate of platelets from patients with angina was higher than that in healthy controls (10.9% +/- 3.9% versus 5.6% +/- 1.7%, P < .01). Soluble P-selectin and soluble L-selectin levels of patients with angina significantly increased after passage through the columns. Levels of soluble P-selectin and L-selectin in healthy controls did not exhibit significant changes with passage through the columns. These results suggested that high shear stress caused abnormal activation of leukocytes or adhesive proteins in patients with effort angina, and ticlopidine failed to suppress hyperaggregability of platelets in these patients.
authors.affiliation: Second Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
authors.forename: Norihito
authors.initials: N
authors.lastname: Inami
authors.forename: Shosaku
authors.initials: S
authors.lastname: Nomura
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kimura
authors.forename: Kohichi
authors.initials: K
authors.lastname: Yamada
authors.forename: Hisato
authors.initials: H
authors.lastname: Nakamori
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Takahashi
authors.forename: Shirou
authors.initials: S
authors.lastname: Fukuhara
authors.forename: Toshiji
authors.initials: T
authors.lastname: Iwasaka
mesh_terms: D000368:Aged; D000787:Angina Pectoris / Q000097:blood* / Q000188:drug therapy / Q000628:therapy; D001241:Aspirin / Q000627:therapeutic use; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D016022:Case-Control Studies; D003094:Collagen; D005260:Female; D018056:Hemorheology; D006801:Humans; D066298:In Vitro Techniques; D019041:L-Selectin / Q000097:blood; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood; D010316:Particle Size; D010975:Platelet Aggregation Inhibitors / Q000627:therapeutic use; D010979:Platelet Function Tests / Q000379:methods*; D012995:Solubility; D015607:Stents; D013988:Ticlopidine / Q000627:therapeutic use
chemical_list: D019007:P-Selectin; D010975:Platelet Aggregation Inhibitors; D019041:L-Selectin; D003094:Collagen; D013988:Ticlopidine; D001241:Aspirin