[Genetic polymorphism of twelve Y chromosomal short tandem repeat loci in Chinese Hui ethnic group].
OBJECTIVE
To obtain the genetic polymorphism of Y chromosomal short tandem repeat (Y-STR) loci in Ningxia Hui population.


METHODS
Blood samples were collected from 150 unrelated healthy male individuals of Ningxia Hui ethnic group. Twelve Y-STR loci were amplified in one tube by using the PowerPlex System STR Amplification Kit, and the genotypes were determined using Genescan and Genotype software of ABI377 DNA sequencer and the frequency of alleles and haplotypes of Ningxia Hui ethnic was obtained.


RESULTS
Seventy-five alleles were observed at 12 Y-STR loci. The frequency ranged from 0.0067-0.7067 and the gene diversity ranged from 0.4446-0.8877. Totally 148 different haplotypes were found, which were unique in 150 males. Two haplotypes were shared by 2 males respectively. The haplotype diversity was 0.9864.


CONCLUSIONS
The 12 Y-STR loci are highly polymorphic in Ningxia Hui population and are suitable for genetics and forensic research.
Zhu
Yong-sheng
YS
Key Laboratory of Reproduction and Genetics in Ningxia, Department of Medical Genetics and Cell Biology, Ningxia Medical College, Yingchuan, Ningxia, 750004 PR China.
Huo
Zheng-hao
ZH
Yu
Bing
B
Zhang
Hong-bo
HB
Wang
Yu-jiong
YJ
Zhao
Wei
W
Jiao
Hai-yan
HY
Dang
Jie
J
Li
Sheng-bin
SB
D044466:Asian People / Q000235:genetics*; D002681:China / Q000208:ethnology; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic