title: The role of CCL22/macrophage-derived chemokine in allergic rhinitis.
abstract: Dendritic cells (DCs) are considered to be the most powerful antigen-presenting cells (APCs). DCs are thought to be associated with Th1 or Th2 polarization and with polarization-induced disease such as atopic dermatitis, asthma and allergic rhinitis, but its mechanism is not well known. In this study, we analyzed the mRNA expression of DCs between birch pollen allergic rhinitis and healthy controls by using cDNA array. We found that the expressions of CCL22/macrophage-derived chemokine (MDC) differed significantly. We also revealed that CCL22/MDC production was higher in patients than in healthy donors. By chemotaxis assay, CCL22/MDC can enhance the migration of patient's T cells rather than those of healthy controls. Surface marker analysis of migrated cells revealed that the most of migrated cells expressed CCR4, which were considered to be Th2 cells. Furthermore, CD1a(+) CD83(+) cells located in the nasal mucosa expressed CCL22/MDC in vivo. To the best of our knowledge, this is the first report clearly indicating the role of CCL22/MDC in allergic rhinitis.
authors.affiliation: Department of Pathology, Asahikawa Medical College, Hokkaido, Japan. mitsuru@asahikawa-med.ac.jp
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Yanai
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sato
authors.forename: Naoko
authors.initials: N
authors.lastname: Aoki
authors.forename: Yumi
authors.initials: Y
authors.lastname: Takiyama
authors.forename: Kensuke
authors.initials: K
authors.lastname: Oikawa
authors.forename: Hiroya
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Shoji
authors.initials: S
authors.lastname: Kimura
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Harabuchi
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Tateno
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis; D029662:Betula / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D054422:Chemokine CCL22 / Q000276:immunology* / Q000378:metabolism; D018925:Chemokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D009297:Nasal Mucosa / Q000166:cytology / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D011058:Pollen / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D054398:Receptors, CCR4 / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology* / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C515354:CCR4 protein, human; D054422:Chemokine CCL22; D018925:Chemokines; D012333:RNA, Messenger; D054398:Receptors, CCR4