title: [Changes of cytokines of splenocytes in mice immunized by mix recombinant BCG-EmII/3 and BCG-Em14-3-3 vaccine of Em].
abstract: OBJECTIVE
  To investigate the changes of cytokines of splenocytes in mice immunized by mix recombinant BCG-EmII/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis(Em) and challenged by Em protoscoleces.
  
  
  METHODS
  BALB/c mice were vaccinated subcutaneously and intranasally by the vaccine respectively. In the eighth week of vaccination they were Challenged by Em protoscoleces.In the eighteenth week of infection they were killed for spleen. After splenocytes were separated,their culture was stimulated by EmAg or ConA and their supernatants were gathered. The levels of IL-2, IFN-gamma, TNF-alpha and IL-4 were measured by ELISA kit. Blank vector, BCG and PBS were served as control.
  
  
  RESULTS
  In the groups of vaccine immunization, the levels of IFN-gamma and TNF-alpha increased obviously but the level of IL-4 decreased. The level of TNF-alpha in subcutaneous group was higher than that in intranasal group.
  
  
  CONCLUSIONS
  Th1 response was induced in mice immunized by mix recombinant BCG-EmII/3 and BCG-Em14-3-3 vaccine of Em against the challenge by Em protoscoleces. Subcutaneous injection with this vaccine may be a good way of vaccination.
authors.affiliation: Institute of Infectious and Parasitic Diseases, First Affiliated Hospital, Chongqing Medical University, Chongqing, China. li_wengui@yahoo.com.cn
authors.forename: Wen-Gui
authors.initials: WG
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: You-Ming
authors.initials: YM
authors.lastname: Zhu
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D016207:Cytokines / Q000276:immunology / Q000378:metabolism*; D004274:DNA, Recombinant; D048210:Echinococcus multilocularis / Q000235:genetics* / Q000276:immunology*; D005260:Female; D007279:Injections, Subcutaneous; D051379:Mice; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis / Q000235:genetics*; D013154:Spleen / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D014611:Vaccination
chemical_list: D001428:Bacterial Vaccines; D016207:Cytokines; D004274:DNA, Recombinant