title: Immunolocalization of androgen and oestrogen receptors in the ventral lobe of rat (Rattus norvegicus) prostate after long-term treatment with ethanol and nicotine.
abstract: Nicotine and alcohol adversely affect prostate gland function. In this work, immunohistochemistry was used to investigate the immunoreactivity and distribution of androgen and alpha, beta-oestrogen receptors following chronic treatment with alcohol, nicotine or a combination of both substances, as well as to relate these results to the development of possible prostatic pathologies. Forty male rats were divided into four groups: the Control group received tap water; the Alcoholic group received diluted 10% Gay Lussac ethanol; the Nicotine group received a 0.125 mg/100 g body weight dose of nicotine injected subcutaneously on a daily basis (Sigma Chemical Company, St. Louis, MO, USA); the Nicotine-Alcohol group received simultaneous alcohol and nicotine treatment. After 90 days of treatment, samples of the ventral lobe of the prostate were collected and processed for immunohistochemistry, light microscopy and the quantification of serum hormonal concentrations. The results showed significantly decreased serum testosterone levels and increased serum oestrogen levels in the animals from the nicotine-alcohol, the alcoholic and the nicotine groups, as well as their hormonal receptor levels. Then, it was concluded that ethanol and nicotine compromised the prostatic hormonal balance, which is a crucial factor to maintain the morphological and physiological features of this organ.
authors.affiliation: Department of Anatomy, Institute of Biology, State University of Campinas, São Paulo, Brazil.
authors.forename: W J
authors.initials: WJ
authors.lastname: Fávaro
authors.forename: V H A
authors.initials: VH
authors.lastname: Cagnon
mesh_terms: D000818:Animals; D047628:Estrogen Receptor alpha / Q000032:analysis; D047629:Estrogen Receptor beta / Q000032:analysis; D004967:Estrogens / Q000097:blood; D000431:Ethanol / Q000494:pharmacology*; D008297:Male; D009538:Nicotine / Q000494:pharmacology*; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011944:Receptors, Androgen / Q000032:analysis*; D011960:Receptors, Estrogen / Q000032:analysis*; D013739:Testosterone / Q000097:blood
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D000431:Ethanol; D013739:Testosterone; D009538:Nicotine