title: Electrokinetic injection of DNA from gel micropads: basis for coupling polony technology with CE separation.
abstract: We propose a novel method for electrokinetic injection of DNA samples into capillaries from nanoliter gel micropads, deposited on glass slides, which are coated with electroconducting film. Theoretical and experimental proof is presented for the proposed method. The method allows efficient and highly precise injection without physical contact between the gel pad and the capillary. Read length of more than 700 bp at Q20 has been reproducibly demonstrated in fused-silica capillaries using the proposed injection technique. Based on the obtained results we discuss a novel DNA sequencing system which combines DNA amplification and cycle sequencing in arrays of subnanoliter gel micropads and high-throughput electrophoretic separation in monolith multicapillary arrays.
authors.affiliation: Department of Electrical and Computer Engineering, State University of New York, Stony Brook, NY 11794-2350, USA. okey@ece.sunysb.edu
authors.forename: Olga
authors.initials: O
authors.lastname: Kosobokova
authors.forename: Dmitri N
authors.initials: DN
authors.lastname: Gavrilov
authors.forename: Vyacheslav
authors.initials: V
authors.lastname: Khozikov
authors.forename: Andrey
authors.initials: A
authors.lastname: Stepukhovich
authors.forename: Andriy
authors.initials: A
authors.lastname: Tsupryk
authors.forename: Sergey
authors.initials: S
authors.lastname: Pan'kov
authors.forename: Olga
authors.initials: O
authors.lastname: Somova
authors.forename: Nikolai
authors.initials: N
authors.lastname: Abanshin
authors.forename: Georgiy
authors.initials: G
authors.lastname: Gudkov
authors.forename: Marina
authors.initials: M
authors.lastname: Tcherevishnik
authors.forename: Vera
authors.initials: V
authors.lastname: Gorfinkel
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification*; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D005782:Gels / Q000737:chemistry; D007202:Indicators and Reagents; D008956:Models, Chemical; D036103:Nanotechnology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005782:Gels; D007202:Indicators and Reagents; D004247:DNA