Different response between production of free radicals induced by central and peripheral administration of interleukin-1beta in conscious rats.
We examined whether central or peripheral administration of interleukin-1beta (IL-1beta) might change levels of nitric oxide (NO) and hydroxyl radical (*OH) in the medial prefrontal cortex (mPFC). Extracellular levels of NO metabolites (NOx(-)) and 2,3-dihydroxybenzoic acid (2,3-DHBA), as a marker of *OH production, were determined with an in vivo microdialysis technique in conscious rats. In the mPFC, central administration of IL-1beta into the mPFC resulted in dose-dependent increases in levels of both NOx(-) and 2,3-DHBA. In contrast, peripheral administration of IL-1beta significantly increased NOx(-) levels but not 2,3-DHBA levels. Perfusion of Mn(III) tetrakis (4-benzoic acid) porphyrin chloride, a superoxide (O(2)(-)) dismutase mimic, into the mPFC reduced the increases in levels of 2,3-DHBA induced by centrally administered IL-1beta, but enhanced the increases in levels of NOx(-) induced by centrally administered IL-1beta. The present results show a different response in free radical productions in the mPFC between central and peripheral administration of IL-1beta. This finding should be useful for our understanding of the response of NO and free radicals such as *OH and O(2)(-) in the mPFC after central and peripheral administration of IL-1beta.
Ishizuka
Yuta
Y
Department of Psychiatry, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Miyazaki 889-1692, Japan. ishizuka@fc.miyazaki-u.ac.jp
Abe
Hiroshi
H
Nakane
Hideyuki
H
Kannan
Hiroshi
H
Ishida
Yasushi
Y
D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D003243:Consciousness / Q000502:physiology; D016503:Drug Delivery Systems; D045604:Extracellular Fluid / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism*; D062385:Hydroxybenzoates / Q000378:metabolism; D053583:Interleukin-1beta / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D008665:Metalloporphyrins / Q000494:pharmacology; D017551:Microdialysis; D008845:Microinjections; D009569:Nitric Oxide / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D017397:Prefrontal Cortex / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D015415:Biomarkers; D016166:Free Radical Scavengers; D005609:Free Radicals; D062385:Hydroxybenzoates; D053583:Interleukin-1beta; D008665:Metalloporphyrins; C097284:manganese(III)-tetrakis(4-benzoic acid)porphyrin; D009569:Nitric Oxide; C009135:2,3-dihydroxybenzoic acid