Structure of Mycobacterium tuberculosis mtFabD, a malonyl-CoA:acyl carrier protein transacylase (MCAT).
Mycobacteria display a unique and unusual cell-wall architecture, central to which is the membrane-proximal mycolyl-arabinogalactan-peptidoglycan core (mAGP). The biosynthesis of mycolic acids, which form the outermost layer of the mAGP core, involves malonyl-CoA:acyl carrier protein transacylase (MCAT). This essential enzyme catalyses the transfer of malonyl from coenzyme A to acyl carrier protein AcpM, thus feeding these two-carbon units into the chain-elongation cycle of the type II fatty-acid synthase. The crystal structure of M. tuberculosis mtFabD, the mycobacterial MCAT, has been determined to 3.0 A resolution by multi-wavelength anomalous dispersion. Phasing was facilitated by Ni2+ ions bound to the 20-residue N-terminal affinity tag, which packed between the two independent copies of mtFabD.
Ghadbane
Hemza
H
School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, England.
Brown
Alistair K
AK
Kremer
Laurent
L
Besra
Gurdyal S
GS
Fütterer
Klaus
K
D000123:Acetyltransferases / Q000737:chemistry / Q000378:metabolism; D000213:Acyl Carrier Protein / Q000737:chemistry / Q000378:metabolism; D051080:Acyl-Carrier Protein S-Malonyltransferase / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D002384:Catalysis; D054889:Fatty Acid Synthase, Type II; D064429:Fatty Acid Synthases / Q000737:chemistry / Q000378:metabolism; D008316:Malonyl Coenzyme A / Q000737:chemistry* / Q000378:metabolism; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000201:enzymology*
C431823:AcpM protein, Mycobacterium tuberculosis; D000213:Acyl Carrier Protein; D001426:Bacterial Proteins; D002352:Carrier Proteins; D009097:Multienzyme Complexes; D008316:Malonyl Coenzyme A; D000123:Acetyltransferases; D051080:Acyl-Carrier Protein S-Malonyltransferase; D064429:Fatty Acid Synthases; D054889:Fatty Acid Synthase, Type II