title: Glutathione S-transferase theta 1 expressed in granulosa cells as a biomarker for oocyte quality in age-related infertility.
abstract: OBJECTIVE
  The goal of this study was to identify a reliable biomarker for age-related infertility.
  
  
  METHODS
  Laboratory study.
  
  
  METHODS
  ART laboratory.
  
  
  METHODS
  Patients undergoing intracytoplasmic sperm injection or IVF cycles.
  
  
  METHODS
  Expression of Glutathione S-transferase (GST) mRNA and protein in mural and cumulus granulosa cells obtained from infertile patients were examined by reverse transcriptase-polymerase chain reaction and immunofluorescence.
  
  
  METHODS
  Correlation between the expression of GST theta 1 (GSTT1) in granulosa cells and oocyte quality was a main outcome measure.
  
  
  RESULTS
  Expression of GSTT1 in granulosa cells from male factor patients was positively correlated with age and negatively with cumulus-oocyte complex maturity. When samples with high and low GSTT1 in granulosa cells were extracted from the other infertility factors, cumulus-oocyte complex maturity in the high GSTT1 group was significantly lower than that in the low GSTT1 group (high: 27.2% vs. low: 51.3%). The developmental capacity of oocytes in the high GSTT1 group was likely to be lower (high: 26.4% vs. low: 43.9%). Up-regulation of GSTT1 during aging may be promoted by FSH and H(2)O(2), determined by an in vitro model.
  
  
  CONCLUSIONS
  GSTT1 is a good indicator for age-related infertility.
authors.affiliation: Department of Perinatal Medicine and Maternal Care, Division of Reproductive Medicine, National Center for Child Health and Development, Tokyo, Japan.
authors.forename: Megumu
authors.initials: M
authors.lastname: Ito
authors.forename: Miho
authors.initials: M
authors.lastname: Muraki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Misa
authors.initials: M
authors.lastname: Imai
authors.forename: Tohru
authors.initials: T
authors.lastname: Tsukui
authors.forename: Naomi
authors.initials: N
authors.lastname: Yamakawa
authors.forename: Koji
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Shirei
authors.initials: S
authors.lastname: Ohgi
authors.forename: Takashi
authors.initials: T
authors.lastname: Horikawa
authors.forename: Wakako
authors.initials: W
authors.lastname: Iwasaki
authors.forename: Ayumi
authors.initials: A
authors.lastname: Iida
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nishi
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Yanase
authors.forename: Hajime
authors.initials: H
authors.lastname: Nawata
authors.forename: Kenji
authors.initials: K
authors.lastname: Miyado
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kono
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Hosoi
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Saito
mesh_terms: D000328:Adult; D000375:Aging / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism*; D006107:Granulosa Cells / Q000201:enzymology*; D006801:Humans; D007247:Infertility, Female / Q000201:enzymology* / Q000473:pathology*; D009865:Oocytes / Q000201:enzymology* / Q000473:pathology*
chemical_list: D015415:Biomarkers; C413545:glutathione S-transferase T1; D005982:Glutathione Transferase