Laboratory formulated magnetic nanoparticles for enhancement of viral gene expression in suspension cell line.
One factor critical to successful gene therapy is the development of efficient delivery systems. Although advances in gene transfer technology including viral and non-viral vectors have been made, an ideal vector system has not yet been constructed. Due to the growing concerns over the toxicity and immunogenicity of viral DNA delivery systems, DNA delivery via improve viral routes has become more desirable and advantageous. The ideal improve viral DNA delivery system should be a synthetic materials plus viral vectors. The materials should also be biocompatible, efficient, and modular so that it is tunable to various applications in both research and clinical settings. The successful steps towards this improve viral DNA delivery system is demonstrated: a magnetofection system mediated by modified cationic chitosan-coated iron oxide nanoparticles. Dense colloidal cationic iron oxide nanoparticles serve as an uptake-enhancing component by physical concentration at the cell surface in presence of external magnetic fields; enhanced viral gene expression (3-100-fold) due to the particles is seen as compared to virus vector alone with little virus dose.
Bhattarai
Shanta Raj
SR
Department of Bionanosystem Engineering, Chonbuk National University, Jeonju, South Korea.
Kim
Sun Young
SY
Jang
Kyu Yun
KY
Lee
Ki Chang
KC
Yi
Ho Keun
HK
Lee
Dae Yeol
DY
Kim
Hak Yong
HY
Hwang
Pyoung Han
PH
D045744:Cell Line, Tumor; D005290:Ferric Compounds; D015870:Gene Expression; D005814:Genes, Viral; D005822:Genetic Vectors; D006801:Humans; D008280:Magnetics; D053758:Nanoparticles; D014162:Transfection / Q000379:methods*
D005290:Ferric Compounds; C000499:ferric oxide