title: Amperometric glucose biosensors based on layer-by-layer assembly of chitosan and glucose oxidase on the Prussian blue-modified gold electrode.
abstract: A glucose biosensor based on layer-by-layer (LBL) self-assembling of chitosan and glucose oxidase (GOD) on a Prussian blue film was developed. First, Prussian blue was deposited on a cleaned gold electrode then chitosan and GOD were assembled alternately to construct a multilayer film. The resulting amperometric glucose biosensor exhibited a fast response time (within 10 s) and a linear calibration range from 6 microM to 1.6 mM with a detection limit of 3.1 microM glucose (s/n = 3). With the low operating potential, the biosensor showed little interference to the possible interferents, including ascorbic acid, acetaminophen and uric acid, indicating an excellent selectivity.
authors.affiliation: Chongqing Key Laboratory of Analytical Chemistry, College of Chemistry and Engineering, Southwest University, Chongqing 400715, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Yin
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.forename: Yaqing
authors.initials: Y
authors.lastname: Chai
authors.forename: Shihong
authors.initials: S
authors.lastname: Chen
authors.forename: Shurui
authors.initials: S
authors.lastname: Cao
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Ping
authors.initials: P
authors.lastname: Fu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D048271:Chitosan / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry; D005947:Glucose / Q000032:analysis / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*
chemical_list: D004396:Coloring Agents; D005295:Ferrocyanides; D006046:Gold; D048271:Chitosan; D005949:Glucose Oxidase; D005947:Glucose; C000170:ferric ferrocyanide