Effects of nano-anatase on spectral characteristics and distribution of LHCII on the thylakoid membranes of spinach.
In the article, we report that effects of nano-anatase on the spectral characteristics and content of light-harvesting complex II (LHCII) on the thylakoid membranes of spinach were investigated. The results showed that nano-anatase treatment could increase LHCII content on the thylakoid membranes of spinach and the trimer of LHCII; nano-anatase could enter the spinach chloroplasts and bind to PSII. Meanwhile, spectroscopy assays indicated that the absorption intensity of LHCII from nano-anatase-treated spinach was obviously increased in the red and the blue region, fluorescence quantum yield near 685 nm of LHCII was enhanced, the fluorescence excitation intensity near 440 and 480 nm of LHCII significantly rose and F 480/F 440 ratio was reduced. Oxygen evolution rate of PSII was greatly improved. Together, nano-anatase promoted energy transferring from chlorophyll (chl) b and carotenoid to chl a, and nano-anatase TiO2 was photosensitized by chl of LHCII, which led to enhance the efficiency of absorbing, transferring, and converting light energy.
Zheng
Lei
L
College of Life Sciences, Suzhou University, Suzhou 215123, People's Republic of China.
Lei
Zheng
Z
Su
Mingyu
M
Mingyu
Su
S
Wu
Xiao
X
Xiao
Wu
W
Liu
Chao
C
Chao
Liu
L
Qu
Chunxiang
C
Chunxiang
Qu
Q
Chen
Liang
L
Liang
Chen
C
Huang
Hao
H
Hao
Huang
H
Liu
Xiaoqing
X
Xiaoqing
Liu
L
Hong
Fashui
F
Fashui
Hong
H
D002736:Chloroplasts / Q000378:metabolism; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D018724:Spinacia oleracea; D020524:Thylakoids / Q000737:chemistry / Q000187:drug effects*; D014025:Titanium / Q000378:metabolism / Q000494:pharmacology*
D045342:Light-Harvesting Protein Complexes; D045332:Photosystem II Protein Complex; C009495:titanium dioxide; D014025:Titanium; D010100:Oxygen