Separation and free radical-scavenging activity of major curcuminoids of Curcuma longa using HPTLC-DPPH method.
A direct HPTLC assay was developed for the determination of total curcuminoids and three individual curcuminoids, curcumin, demethoxycurcumin and bisdemethoxycurcumin. In addition, a new procedure was developed to separate and quantitative the free radical-scavenging activity of individual compounds from the rhizome of Curcuma longa L. (Zingiberaceae) based on the combination of HPTLC with a diode array detector (DAD) and post chromatographic DPPH(*) radical derivatisation. It was established that both individual curcuminoids and the extract of C. longa were capable of scavenging DPPH(*) radicals. From the estimated ID(50) values, it can be seen that the order of activity was curcumin > demethoxycurcumin > bisdemethoxycurcumin >> ascorbic acid. However, the ID(50) values of curcuminoids were not significantly different. The data indicates the presence of a synergistic mechanism of antiradical activity of curcuminoids.
Pozharitskaya
Olga N
ON
Interregional Center Adaptogen, 47/5, Piskarevsky pr., 195067, St-Petersburg, Russia.
Ivanova
Svetlana A
SA
Shikov
Alexander N
AN
Makarov
Valery G
VG
D001713:Biphenyl Compounds; D002855:Chromatography, Thin Layer; D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000031:analogs & derivatives* / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry; D015394:Molecular Structure; D010851:Picrates / Q000737:chemistry*
D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl; D003474:Curcumin