title: Neuropathological alteration of aquaporin 1 immunoreactive enteric neurons in the streptozotocin-induced diabetic rats.
abstract: Long-term diabetic patients exhibit major clinical gastrointestinal problems, such as diarrhea and constipation. In recent years, water channel protein, aquaporin 1 (AQP1) has been identified in the enteric nervous system (ENS). We have examined the pathological changes in AQP1 immunoreactive (IR) neurons in streptozotocin-induced (STZ) diabetic rats. Eight-week-old Wistar rats were injected with streptozotocin, and artificial diabetes was induced. Sixteen-week-old STZ rats were then examined with double immunofluorescence staining and ABC immunohistochemical staining. AQP1-IR neurons in STZ rats were significantly increased compared with control rats (p<0.01). The ratio of AQP1 vs. HuC/D in STZ rats was also clearly increased as compared with control rats (p<0.05). It was apparent that thick AQP1-IR fibers were frequently observed in the secondary and tertiary myenteric plexus of STZ rats. The AQP1-IR fibers of STZ rats conspicuously showed many swollen varicosities. These swollen varicose fibers were also observed in the longitudinal and circular muscle layers. Streptozotocin-induced diabetic rats showed pathological changes in AQP1-IR neurons of the ENS. The alteration of AQP1-IR neurons may be possible contribute to diabetic gastrointestinal dysfunction in streptozotocin-induced diabetic rats.
authors.affiliation: Suzuka University of Medical Science, Faculty of Health Science, 1001-1 Kishioka-cho, Suzuka, Mie 510-0293, Japan. e-ishiha@suzuka-u.ac.jp
authors.forename: Eriko
authors.initials: E
authors.lastname: Ishihara
authors.forename: Masato
authors.initials: M
authors.lastname: Nagahama
authors.forename: Satoru
authors.initials: S
authors.lastname: Naruse
authors.forename: Reiji
authors.initials: R
authors.lastname: Semba
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Miura
authors.forename: Masaru
authors.initials: M
authors.lastname: Usami
authors.forename: Masaaki
authors.initials: M
authors.lastname: Narita
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism*; D001369:Axons / Q000378:metabolism / Q000473:pathology; D002452:Cell Count; D048909:Diabetes Complications / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D017615:Enteric Nervous System / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D005455:Fluorescent Antibody Technique; D005767:Gastrointestinal Diseases / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000294:innervation / Q000503:physiopathology; D008297:Male; D009130:Muscle, Smooth / Q000294:innervation; D009197:Myenteric Plexus / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation / Q000502:physiology
chemical_list: C496022:Aqp1 protein, rat; D051398:Aquaporin 1