[Studies on the extraction process of total saponins from Paris polyphylla Smith].
OBJECTIVE
To optimize the extraction process of total saopnins from Paris polyphylla Smith.


METHODS
The single factor test and orthogonal experiment were used to determine the optimum extraction process.


RESULTS
The optimum extraction process was obtained as follows: the plant materials were extracted with 70% ethanol twice, respectively with 10BV for 2 hours and then with 8BV the solvents for 1.5 hours. The yield of total saponins could be up to 4.24% and the total extraction rate of Paris polyphylla I and Paris polyphylla II was 93.28%.


CONCLUSIONS
The optimum process obtained is steady, reasonable and feasible.
Sun
Zhi-Guo
ZG
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Zhang
Lin
L
Li
Ling-Jun
LJ
Tian
Jing-Kui
JK
D000704:Analysis of Variance; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D000431:Ethanol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D019668:Liliaceae / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011208:Powders; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D011208:Powders; D012503:Saponins; D012997:Solvents; D000431:Ethanol