Electron spin resonance analysis of superoxide anion radical scavenging activity with spin trapping agent, diphenyl-PMPO.
Recently, a novel electron spin resonance (ESR) spin-trapping agent, 2-(diphenylphosphinoyl)-2-methyl-3,4-dihydro-2H-pyrrole N-oxide (Diphenyl-PMPO), was synthesized. Because it had some advantages in stability and reactivity over conventional spin-trapping agents, we applied it to the ESR analyses of superoxide anion radical scavenging activity (SOSA) of superoxide dismutase (SOD) and of well-known natural antioxidants (green tea, oolong tea, and red wine). At the same time, the results with Diphenyl-PMPO were compared with 5,5-dimethyl-1-pyrroline N-oxide (DMPO). Our results revealed that Diphenyl-PMPO showed higher detectability than DMPO in the SOSA assays of SOD and natural antioxidants, so it could be available for the ESR analyses as an alternative to conventional spin-trapping agents.
Shimamura
Tomoko
T
Department of Bioresources Science, Faculty of Agriculture, Kochi University, Monobe-B 200, Nankoku, Kochi 783-8502, Japan. tomokos@kochi-u.ac.jp
Fujimura
Yume
Y
Ukeda
Hiroyuki
H
D003497:Cyclic N-Oxides / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D019097:Spin Trapping; D013481:Superoxides / Q000737:chemistry*; D013997:Time Factors
C525532:2-(diphenylphosphinoyl)-2-methyl-3,4-dihydro-2H-pyrrole N-oxide; D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D011758:Pyrroles; D013481:Superoxides