Mutation analysis of p31comet gene, a negative regulator of Mad2, in human hepatocellular carcinoma.
Failure of mitotic checkpoint machinery leads to the chromosomal missegregation and nuclear endoreduplication, thereby driving the emergence of aneuploidy and tetraploidy population. Although abnormal nuclear ploidy and the resulting impairment of mitotic checkpoint function are typical physiological event leading to human hepatocellular carcinoma, any mutational change of mitotic checkpoint regulators has not yet been discovered. Therefore, we investigated the mutation of p31(comet), a recently identified mitotic checkpoint regulator, in human hepatocellular carcinoma. Of 51 human hepatocellular carcinoma tissue and 6 cell lines tested, five samples exhibited nucleotide sequence variations dispersed on four sites within the entire coding sequence. Among these sites with sequence substitutions, three were found to be missense mutation accompanied with amino acid change but one was a silent mutation. Of these sequence substitutions, two were present in both tumor and non-tumor liver tissues, suggesting the possibility of polymorphism. The present findings indicate that p31(comet) does not have an impact on the formation of aneuploidy and tetraploidy found in human hepatocellular carcinoma.
Yun
Mi-Yong
MY
Laboratory of Radiation Molecular Oncology, Division of Radiation Cancer Biology, Korea Institute of Radiological and Medical Sciences, Department of Biochemistry, College of Medicine, Korea University, Seoul 136-701, Korea.
Kim
Sang Bum
SB
Park
Sunhoo
S
Han
Chul Ju
CJ
Han
Young-Hoon
YH
Yoon
Sun Hee
SH
Kim
Sang Hoon
SH
Kim
Chang-Min
CM
Choi
Dong-Wook
DW
Cho
Myung-Haing
MH
Park
Gil-Hong
GH
Lee
Kee-Ho
KH
D048868:Adaptor Proteins, Signal Transducing; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism*; D064246:Mad2 Proteins; D009154:Mutation; D009687:Nuclear Proteins; D011123:Polyploidy; D012097:Repressor Proteins / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C577697:MAD2L1 protein, human; C470309:MAD2L1BP protein, human; D064246:Mad2 Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins