DMSO increases radioiodination yield of radiopharmaceuticals.
A high-yield radioiodination method for various types of molecules is described. The approach employs DMSO as precursor solvent, a reaction ratio of 2-5 precursor molecules per iodine atom, 5-10 microg oxidant, and a 10-25 microl reaction volume. The solution is vortexed at room temperature for 1-5 min and progress of the reaction is assessed by HPLC. Radioiodinated products are obtained in > or = 95% yield and meet the requirements for radiotracer imaging, biodistribution studies, and molecular and cellular biology research.
Wang
Ketai
K
Department of Radiology, Armenise Building Room D2-137, 200 Longwood Avenue, Harvard Medical School, Boston, MA 02115, USA.
Adelstein
S James
SJ
Kassis
Amin I
AI
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000912:Antibodies, Neoplasm / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004121:Dimethyl Sulfoxide; D007074:Immunoglobulin G / Q000302:isolation & purification; D007457:Iodine Radioisotopes / Q000302:isolation & purification*; D051379:Mice; D011854:Radiochemistry; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000302:isolation & purification*; D012997:Solvents
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; C091202:B72.3 antibody; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals; D012997:Solvents; D004121:Dimethyl Sulfoxide