title: Characterisation of 3-methylorcinaldehyde synthase (MOS) in Acremonium strictum: first observation of a reductive release mechanism during polyketide biosynthesis.
abstract: Isolation and sequencing of a PKS gene isolated from xenovulene-producing cultures of Acremonium strictum indicated the presence of NT-, KS-, AT-, PT-, C-MeT- and R-domains; heterologous expression in Aspergillus oryzae resulted in the production of 3-methylorcinaldehyde, demonstrating the role of the terminal reductase domain in product release.
authors.affiliation: School of Biological Sciences, University of Bristol, Woodland Road, Bristol, UK BS8 1UG.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Bailey
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Cox
authors.forename: Kate
authors.initials: K
authors.lastname: Harley
authors.forename: Colin M
authors.initials: CM
authors.lastname: Lazarus
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Simpson
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Skellam
mesh_terms: D000164:Acremonium / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000447:Aldehydes / Q000737:chemistry* / Q000378:metabolism*; D018942:Macrolides / Q000737:chemistry* / Q000378:metabolism*; D008745:Methylation; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010802:Phylogeny
chemical_list: D000447:Aldehydes; D018942:Macrolides