title: Activation of granulocytes by direct interaction with dendritic cells.
abstract: Granulocytes from human peripheral blood were co-cultured with conventional dendritic cells (cDC) or plasmacytoid DCs (pDC) to examine the effects of DCs on the activation or function of granulocytes. After co-culture of granulocytes with DCs, expression of the activation markers of granulocytes (CD63 and CD64) was up-regulated, and increased expression of CD50, the activation marker and ligand for CD209 (DC-SIGN) was also observed. The interaction of granulocytes with DCs was visualized as the cluster where DCs, especially cDCs, were surrounded by granulocytes to form a 'rosette'. After co-culture of granulocytes with cDCs, the secretion of elastase from granulocytes was enhanced significantly when examined cytohistochemically and by enzyme-linked immunosorbent assay. An increase in myeloperoxidase (another activation index of granulocytes) was also observed after co-culture with DCs. These findings suggest the functional and phenotypical activation of granulocytes by interaction with DCs. Furthermore, we examined the involvement of adhesion molecules in the granulocyte-DC interaction, and found that CD209 participates to some extent in this interaction.
authors.affiliation: First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tsuda
authors.forename: M
authors.initials: M
authors.lastname: Inaba
authors.forename: Y
authors.initials: Y
authors.lastname: Sakaguchi
authors.forename: J
authors.initials: J
authors.lastname: Fukui
authors.forename: Y
authors.initials: Y
authors.lastname: Ueda
authors.forename: M
authors.initials: M
authors.lastname: Omae
authors.forename: Y
authors.initials: Y
authors.lastname: Ando
authors.forename: H
authors.initials: H
authors.lastname: Mukaide
authors.forename: K
authors.initials: K
authors.lastname: Guo
authors.forename: T
authors.initials: T
authors.lastname: Yabuki
authors.forename: T
authors.initials: T
authors.lastname: Nakatani
authors.forename: S
authors.initials: S
authors.lastname: Ikehara
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D002450:Cell Communication; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology*; D006098:Granulocytes / Q000201:enzymology / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D010196:Pancreatic Elastase / Q000378:metabolism; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism; D017452:Receptors, IgG / Q000378:metabolism; D012397:Rosette Formation; D060149:Tetraspanin 30; D015854:Up-Regulation
chemical_list: D015703:Antigens, CD; C554586:CD63 protein, human; D010980:Platelet Membrane Glycoproteins; D017452:Receptors, IgG; D060149:Tetraspanin 30; D010196:Pancreatic Elastase