title: The role of cardiac tissue alignment in modulating electrical function.
abstract: BACKGROUND
  Most cardiac arrhythmias are associated with pathology-triggered ion channel remodeling. However, multicellular effects, for example, exaggerated anisotropy and altered cell-to-cell coupling, can also indirectly affect action potential morphology and electrical stability via changed electrotonus. These changes are particularly relevant in structural heart disease, including hypertrophy and infarction. Recent computational studies showed that electrotonus factors into stability by altering dynamic properties (restitution). We experimentally address the question of how cell alignment and connectivity alter tissue function and whether these effects depend on the direction of wave propagation.
  
  
  RESULTS
  We show that cardiac cell arrangement can alter electrical stability in an in vitro cardiac tissue model by mechanisms both dependent and independent of the direction of wave propagation, and local structural remodeling can be felt beyond a space constant. Notably, restitution of action potential duration (APD) and conduction velocity was significantly steepened in the direction of cell alignment. Furthermore, prolongation of APD and calcium transient duration was found in highly anisotropic cell networks, both for longitudinal and transverse propagation. This is in contrast to expected correlation between wave propagation direction and APD based on electrotonic effects only, but is consistent with our findings of increased cell size and secretion of atrial natriuretic factor, a hypertrophy marker, in the aligned structures.
  
  
  CONCLUSIONS
  Our results show that anisotropic structure is a potent modulator of electrical stability via electrotonus and molecular signaling. Tissue alignment must be taken into account in experimental and computational models of arrhythmia generation and in designing effective treatment therapies.
authors.affiliation: Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York, USA.
authors.forename: Chiung-Yin
authors.initials: CY
authors.lastname: Chung
authors.forename: Harold
authors.initials: H
authors.lastname: Bien
authors.forename: Emilia
authors.initials: E
authors.lastname: Entcheva
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D016880:Anisotropy; D016764:Cell Polarity; D002478:Cells, Cultured; D006329:Heart Conduction System / Q000166:cytology* / Q000502:physiology*; D008955:Models, Cardiovascular; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley