title: The influence of varying fluoride concentrations on in vitro remineralisation of artificial dentinal lesions with differing lesion morphologies.
abstract: OBJECTIVE
  The influence of low-level fluoride (F) concentrations and lesion characteristics on the remineralisation of sub-surface root caries was investigated in vitro.
  
  
  METHODS
  Experimentally produced dentinal carious lesions were exposed to artificial saliva in the presence of 0.00ppm, 0.48ppm, 2.49ppm or 4.91ppm F (as NaF) for 5 days. Calcium, phosphate and fluoride ion uptake was quantified by chemical assay. Baseline changes in the mineral content and distribution of the lesions were assessed by transverse microradiography (TMR).
  
  
  RESULTS
  The uptake of calcium and phosphate was significantly increased (p<0.05) by fluoride, even at low concentrations. The action of fluoride was influenced by the mineral content and distribution of the lesions at baseline, however, with the location and degree of mineral deposition being strongly related to the mineral content of the lesions surface layer.
  
  
  CONCLUSIONS
  The process of remineralisation of root dentine is multifactorial. The morphology of lesions at baseline significantly affected the location and quantity of mineral deposition.
authors.affiliation: School of Dental Sciences, University of Liverpool Dental Hospital and School, Liverpool L3 5PS, UK. katepreston1@hotmail.com
authors.forename: K P
authors.initials: KP
authors.lastname: Preston
authors.forename: P W
authors.initials: PW
authors.lastname: Smith
authors.forename: S M
authors.initials: SM
authors.lastname: Higham
mesh_terms: D002118:Calcium / Q000378:metabolism; D002327:Cariostatic Agents / Q000627:therapeutic use*; D003731:Dental Caries / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003747:Dental Enamel Solubility; D003804:Dentin / Q000378:metabolism* / Q000473:pathology; D003806:Dentin Permeability; D004305:Dose-Response Relationship, Drug; D005460:Fluorides, Topical / Q000627:therapeutic use*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008852:Microradiography; D008963:Molar; D014089:Tooth Remineralization
chemical_list: D002327:Cariostatic Agents; D005460:Fluorides, Topical; D002118:Calcium