title: Effect of different growth factors on human osteoblasts activities: a possible application in bone regeneration for tissue engineering.
abstract: Cultured human primary osteoblasts reproduce the phenotypic differentiation and maturation of cells in vivo. We have investigated the influence of three isoforms of transforming growth factor beta (TGF-beta1, TGF-beta2 and TGF-beta3), three fibroblast growth factors (FGF-2, FGF-4 and FGF-6) and the active metabolite of Vitamin D [1,25-(OH)(2)D3] on proliferation, alkaline phosphatase activity and mineralization of human osteoblasts during a period of 24 days of culture. TGF-beta isoforms and three FGFs examined have been proved to be inducers of osteoblasts proliferation (higher extent for TGF-beta and FGF-2) and inhibitors of alkaline phosphatase activity and osteoblasts mineralization. Combination of these growth factors with the active form of Vitamin D induced osteodifferentiation. In fact Vitamin D showed an additive effect on alkaline phosphatase activity and calcium content, induced by FGF-2 and TGF-beta in human osteoblast. These results highlight the potential of proliferating cytokines' combination with mineralizing agents for in vitro bone growth induction in bone tissue engineering.
authors.affiliation: Department of Clinical and Experimental Medicine, School of Medicine, University of Eastern Piedmont, Via Solaroli 17, 28100 Novara, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Bosetti
authors.forename: Francesca
authors.initials: F
authors.lastname: Boccafoschi
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Leigheb
authors.forename: Mario F
authors.initials: MF
authors.lastname: Cannas
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D002113:Calcification, Physiologic / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D005346:Fibroblast Growth Factors / Q000494:pharmacology* / Q000502:physiology; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D015398:Signal Transduction; D023822:Tissue Engineering; D016212:Transforming Growth Factor beta / Q000494:pharmacology* / Q000502:physiology; D014807:Vitamin D / Q000494:pharmacology* / Q000502:physiology
chemical_list: D016212:Transforming Growth Factor beta; D014807:Vitamin D; D005346:Fibroblast Growth Factors; D000469:Alkaline Phosphatase