title: Association of NET-1 gene expression with human hepatocellular carcinoma.
abstract: NET-1 is a member of the NET-x family. To explore the potential role of NET-1 in hepatocellular carcinoma (HCC), the expression of NET-1 and the relationship with HCC were examined for the first time. We found that NET-1 was frequently expressed in HCC and the peritumor tissue. The relative amounts of NET-1 mRNA in HCC and peritumor tissue were 0.645 +/- 0.37 and 0.466 +/- 0.30, respectively, indicating a higher expression level in HCC than in the peritumor (P < .05). NET-1 protein is usually located on the cell membrane and in the cytoplasm of HCC cells. NET-1 immunoreactivity was found in 126 out of 130 samples of HCC tissue (96.92%). An association of NET-1 expression with cytological variants, histopathological grading, and clinical stages of HCC was also found (P < .05). Detection of NET-1 gene expression in liver biopsy may provide useful information about the biological behavior of HCC.
authors.affiliation: Department of Pathological Anatomy, Affiliated Hospital, Nantong University, 20 Xi Si Road, Nantong, Jiangsu, China 226 001.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Zhan
authors.forename: De-Chuen
authors.initials: DC
authors.lastname: Li
authors.forename: York-Yuan
authors.initials: YY
authors.lastname: Zhu
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhu
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D001706:Biopsy; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009367:Neoplasm Staging; D015513:Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C099763:NET1 protein, human; D015513:Oncogene Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm