A potential immunotherapy approach: mucosal immunization with an IL-13 peptide-based virus-like particle vaccine in a mouse asthma model.
Interleukin (IL)-13 is critical in asthma pathogenesis. Previously, we have developed an IL-13 peptide-based vaccine and confirmed that subcutaneous immunization with the vaccine suppressed airway allergic inflammatory responses in a mouse asthma model. In the present study, we sought to test if mucosal immunization with the vaccine could be a potential approach, by inducing specific autoantibodies of both local IgA in the airway and systemic IgG in serum, to provide an overall suppression of redundant IL-13 effects. The results show that intranasal vaccination induces IL-13-specific IgA responses in multiple mucosal tissues and higher titers of IgG in serum than subcutaneous vaccination. This approach leads to a more effective suppression of ovalbumin-driven Th2 patterns of antibody responses and airway IL-13 and eosinophil accumulation than subcutaneous immunization, even when the induced IL-13 IgG responses were at a similar level. In conclusion, mucosal vaccination may be an innovative potential approach in the treatment of asthma.
Ma
Yanbing
Y
Department of Pediatrics and Child Health, University of Manitoba, 532-715 McDermot Avenue, Winnipeg, Canada R3E 3P4.
Ma
Allan G
AG
Peng
Zhikang
Z
D000818:Animals; D001249:Asthma / Q000276:immunology* / Q000517:prevention & control*; D001324:Autoantigens / Q000276:immunology; D002465:Cell Movement; D004195:Disease Models, Animal; D004804:Eosinophils / Q000166:cytology / Q000276:immunology; D005260:Female; D006512:Hepatitis B Core Antigens / Q000276:immunology; D018928:Immunity, Mucosal / Q000276:immunology*; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D018793:Interleukin-13 / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D011994:Recombinant Proteins / Q000276:immunology; D012261:Ribonucleoproteins / Q000276:immunology; D014611:Vaccination; D014771:Virion / Q000276:immunology* / Q000648:ultrastructure; D000097585:SS-B Antigen
D001324:Autoantigens; D006512:Hepatitis B Core Antigens; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D018793:Interleukin-13; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; D000097585:SS-B Antigen