Antibodies to elastin peptides in sera of Belgian Draught horses with chronic progressive lymphoedema.
BACKGROUND
Chronic progressive lymphoedema (CPL) is a recently recognised disease of the lymphatic system characterised by lesions in the skin of the lower legs in several draught horse breeds, including the Belgian Draught hourse. Clinical signs slowly progress and result in severe disfigurement of the limbs. Ideally, supportive treatment should be started early in the disease process. However early diagnosis and monitoring progression of CPL is still a challenge.


OBJECTIVE
Elastin changes, characterised by morphological alterations as well as increased desmosine levels, in the skin of the distal limbs of horses affected with CPL are probably associated with a marked release of elastin degradation products, which elicit production of circulating anti-elastin antibodies (AEAbs) in the serum. An enzyme-linked immunosorbent assay (ELISA) for detection of serum AEAbs may document elastin breakdown.


METHODS
An ELISA technique was used to evaluate levels of AEAbs in sera of 97 affected Belgian Draught horses that were clinically healthy except for possible skin lesions, associated with CPL in their distal limbs. The horses were divided into 5 groups according to the severity of these skin lesions: normal horses (Group 1, n = 36), horses with mild lesions (Group 2, n = 43), horses with moderate lesions (Group 3, n = 8), horses with severe lesions (Group 4, n = 10) and, as a control, healthy Warmblood horses, unaffected by the disease (Group 5, n = 83).


RESULTS
Horses with clinical signs of CPL had significantly higher AEAb levels compared to clinically normal Belgian Draught horses and to healthy Warmblood horses. These levels correlated with severity of lesions.


CONCLUSIONS
CPL in draught horses is associated with an increase of serum AEAbs.


CONCLUSIONS
Evaluation of serum levels of AEAbs by ELISA might be a useful diagnostic aid for CPL. Pathological degradation of elastic fibres, resulting in deficient support of the distal lymphatics, is proposed as a contributing factor for CPL in Belgian Draught horses.
van Brantegem
L
L
Laboratory of Pathology, Department of Veterinary Medicine, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Wilrijk, Belgium.
de Cock
H E V
HE
Affolter
V K
VK
Duchateau
L
L
Hoogewijs
M K
MK
Govaere
J
J
Ferraro
G L
GL
Ducatelle
R
R
D000375:Aging / Q000378:metabolism / Q000502:physiology; D000818:Animals; D001323:Autoantibodies / Q000097:blood*; D001947:Breeding; D002908:Chronic Disease; D003895:Desmosine / Q000378:metabolism; D003937:Diagnosis, Differential; D018450:Disease Progression; D004549:Elastin / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D005260:Female; D006734:Horse Diseases / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D006736:Horses; D008209:Lymphedema / Q000097:blood / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D008297:Male; D010455:Peptides / Q000097:blood / Q000276:immunology / Q000378:metabolism; D012680:Sensitivity and Specificity; D012867:Skin / Q000473:pathology
D001323:Autoantibodies; D010455:Peptides; D003895:Desmosine; D004549:Elastin