title: Dynamic control of protein folding pathway with a polymer of tunable hydrophobicity.
abstract: While the knowledge of protein folding in a dilute solution is now well-advanced, little is known of the influence of surrounding conditions on the folding kinetics, in particular when the protein is in a dynamically responsive environment. Here we report a new procedure to control the pathways of protein folding by using a thermally responsive polymer that varies its hydrophobicity concomitant with the protein structural changes. The advantages of folding in a dynamic environment have been demonstrated first by Langevin dynamics simulations on the basis of coarse-grained models for both the protein and polymer and then by experiments for lysozyme refolding in the presence of poly(N-isopropylacrylamide-co-N-tert-butylacrylamide), a thermal responsive polymer that varies its hydrophobicity in response to temperature. The simulation suggests that decreasing the polymer hydrophobicity during the folding process may result in an optimized free-energy landscape that enhances both the folding yield and kinetics. The experiments affirm that an optimal folding condition can be identified when structural transitions of the protein collaborate with the polymer hydrophobicity tuned by variation of temperature.
authors.affiliation: Department of Chemical Engineering, Tsinghua University, Beijing, 10084.
authors.forename: Diannan
authors.initials: D
authors.lastname: Lu
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Wu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000178:Acrylamides / Q000494:pharmacology*; D003198:Computer Simulation; D057927:Hydrophobic and Hydrophilic Interactions; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D017510:Protein Folding; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology; D013816:Thermodynamics
chemical_list: D000178:Acrylamides; C079805:poly(N-isopropylacrylamide-N-tert-butylacrylamide)copolymer; D009113:Muramidase