[Association of -63A/C polymorphism of glutathione S-transferase M3 gene with essential hypertension in Chinese population].
OBJECTIVE
To investigate the distribution of -63A/C polymorphism of human glutathione S-transferase M3(GSTM3) gene in Chinese Han population and the association of -63A/C polymorphism with essential hypertension (EH).


METHODS
-63A/C polymorphism of GSTM3 gene was examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 234 patients with EH and 328 healthy controls. The genotypes were confirmed partially by DNA sequencing.


RESULTS
The distribution of GSTM3 -63A/C was in Hardy-Weinberg equilibrium. The CC genotype frequency in EH group (6%) was significantly higher than that in control group(1.8%) (P < 0.05). After adjustment for age, body mass index, and other risk factors, the CC genotype was independently associated with hypertension (OR=3.447, 95%CI: 1.19-7.63; P=0.04).


CONCLUSIONS
The GSTM3 -63A/C polymorphism was associated with EH in Chinese Han population, the C allele might be a risk factor for EH in Chinese Han nationality.
Liu
Jian
J
Department of Medical Genetics, China Medical University, Shenyang, Liaoning, 110001 PR China.
Li
Miao
M
Qi
Man-long
ML
Liu
Xiao-liang
XL
Li
Ying-hui
YH
Fu
Ling-yu
LY
Zhao
Yan-yan
YY
D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D016022:Case-Control Studies; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D006973:Hypertension / Q000235:genetics* / Q000473:pathology; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012044:Regression Analysis
D007527:Isoenzymes; D005982:Glutathione Transferase; C416969:glutathione transferase M3-3