title: Genome-wide analysis of coding DNA and amino acid variation in Saccharomyces cerevisiae.
abstract: The possible causes of variation on amino acid composition in the yeast Saccharomyces cerevisiae were investigated genome-wide. The results indicated that: (a) the base composition of coding DNA and amino acid composition was similar among all the chromosomes, which was in sharp contrast with the great varies of the composition of the individual's coding DNA and amino acid; (b) some amino acids (e.g. Cys and Trp) were not present in all the proteins; and (c) amino acid bias was associated with a base bias (in terms of A-, G-, C- and T-rich codons). Based on the third rule and a proposed universal trend of amino acid gain and loss in protein evolution, the changing pattern of coding DNA was predicted to be T- and C-accruing, whereas A and G were consistently reducing. All these results held the potential to reveal precisely how DNA ongoing change has a major effect on the composition of proteins.
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian 116023, People's Republic of China. hdt@dicp.ac.cn
authors.forename: Haidong
authors.initials: H
authors.lastname: Tan
authors.forename: Jinxia
authors.initials: J
authors.lastname: Wang
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Zongbao K
authors.initials: ZK
authors.lastname: Zhao
mesh_terms: D000596:Amino Acids / Q000235:genetics*; D001482:Base Composition; D004271:DNA, Fungal / Q000235:genetics*; D019143:Evolution, Molecular; D016681:Genome, Fungal; D016014:Linear Models; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D004271:DNA, Fungal; D029701:Saccharomyces cerevisiae Proteins