Preparation of carbohydrate-oligonucleotide conjugates using the squarate spacer.
Attachment of carbohydrates to oligonucleotides has proven to induce receptor-mediated endocytosis. A facile method for the formation of covalent linkages between glycans and oligonucleotides is herein described. Thus, use of 3,4-diethoxy-3-cyclobutene-1,2-dione as a linking reagent provides easy conjugation between carbohydrates bearing an amino group at the reducing end and oligonucleotides bearing an aminoalkyl modification.
Yan
Hongbin
H
Department of Chemistry, Brock University, St. Catharines, Ont., Canada L2S 3A1. tyan@brocku.ca
Aguilar
Aimé López
AL
Zhao
Yuyan
Y
D002241:Carbohydrates / Q000138:chemical synthesis*; D003432:Cross-Linking Reagents / Q000737:chemistry; D003503:Cyclobutanes / Q000138:chemical synthesis*; D016503:Drug Delivery Systems; D006001:Glycoconjugates / Q000138:chemical synthesis*; D006031:Glycosylation; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000378:metabolism / Q000627:therapeutic use; D011134:Polysaccharides / Q000378:metabolism
D002241:Carbohydrates; D003432:Cross-Linking Reagents; D003503:Cyclobutanes; D006001:Glycoconjugates; D009841:Oligonucleotides; D011134:Polysaccharides; C030042:squaric acid