Establishment of a novel equine cell line for isolation and propagation of equine herpesviruses.
In the present study, an equine-derived cell line was established by transfecting primary fetal horse kidney (FHK) cells with expression plasmid encoding simian virus 40 (SV40) large T antigen and then cloning them by limiting dilution. The cloned cell line, named FHK-Tcl3, grew well and could be propagated over 30 times by splitting them 1:3. Equine herpesvirus (EHV)-1 and EHV-4 replicated well in FHK-Tcl3. EHV-2 and EHV-4 were isolated from samples collected from horses in the field using FHK-Tcl3, and EHV-3 also propagated in FHK-Tcl3. These results indicated that this novel cell line, FHK-Tcl3, can be used for isolation and propagation of equine herpesviruses.
Maeda
Ken
K
Laboratory of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan. kmaeda@yamaguchi-u.ac.jp
Yasumoto
Shigeru
S
Tsuruda
Akari
A
Andoh
Kiyohiko
K
Kai
Kazushige
K
Otoi
Takeshige
T
Matsumura
Tomio
T
D000818:Animals; D002460:Cell Line / Q000166:cytology / Q000821:virology*; D002999:Clone Cells; D003588:Cytopathogenic Effect, Viral; D004622:Embryo, Mammalian; D004861:Herpesvirus 1, Equid / Q000254:growth & development* / Q000302:isolation & purification; D023141:Herpesvirus 4, Equid / Q000254:growth & development* / Q000302:isolation & purification; D006734:Horse Diseases / Q000821:virology*; D006736:Horses; D007668:Kidney / Q000166:cytology