Hsp72 release: mechanisms and methodologies.
To date there are two mechanisms that are recognized by which heat shock proteins (HSP) are released from cells; a passive release mechanism, including necrotic cell death, severe blunt trauma, surgery and following infection with lytic viruses, and an active release mechanism which involves the non classical protein release pathway in which HSPs are released within highly immunologically potent exosomes and as free HSP. This chapter describes the experimental procedures that have been developed to study the mechanism by which stress induces the release of HSP72 into the circulation and addresses the biological significance of circulating HSP72 to host defense against disease.
Asea
Alexzander
A
Division of Investigative Pathology, Scott & White Clinic and Texas A&M University System Health Science Center College of Medicine, 1901 South 1st Street, Temple, TX 76504, USA. aasea@swmail.sw.org
D000818:Animals; D002468:Cell Physiological Phenomena; D003713:Dendritic Cells / Q000276:immunology / Q000502:physiology; D050884:HSP72 Heat-Shock Proteins / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D020014:K562 Cells
D050884:HSP72 Heat-Shock Proteins