Identification of proteins directly phosphorylated by UL13 protein kinase from herpes simplex virus 1.
Herpes simplex virus 1 (HSV-1) UL13 is a viral protein kinase that regulates optimal viral replication in cell cultures. Identification of substrates of protein kinases is a crucial step to elucidate the mechanism by which they function. Using our developed system to analyze the specific protein kinase activity of UL13, we have shown that UL13 protein kinase directly phosphorylates the viral proteins ICP22 and UL49 previously reported to be putative substrates. We also identified UL41 as a previously unreported and novel substrate of UL13. These data will serve as a basis to clarify the mechanism by which UL13 influences viral replication.
Asai
Risa
R
Division of Viral Infection, Department of Infectious Disease Control, International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Ohno
Takashi
T
Kato
Akihisa
A
Kawaguchi
Yasushi
Y
D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000378:metabolism; D017874:Immediate-Early Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D018411:Spodoptera; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins / Q000378:metabolism*
C057294:ICP22 protein, human herpesvirus 1; D017874:Immediate-Early Proteins; D011993:Recombinant Fusion Proteins; C081688:Ul41 protein, Human herpesvirus 1; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; D005982:Glutathione Transferase; D011494:Protein Kinases; C082380:UL13 protein, Simplexvirus