Halorubrum litoreum sp. nov., an extremely halophilic archaeon from a solar saltern.
An extremely halophilic archaeon, strain Fa-1(T), was isolated from a marine solar saltern in Fujian, China. Strain Fa-1(T) required Mg2+ and at least 2.0 M NaCl for growth. It was able to grow at pH 6.5-9.0 (optimally at pH 7.0-7.5) and at 20-55 degrees C (optimally at 37-42 degrees C). The major polar lipids of strain Fa-1(T) were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a sulfated diglycosyl diether. On the basis of a 16S rRNA gene sequence analysis, strain Fa-1(T) was closely related to nine species of the genus Halorubrum, showing sequence similarities of 97.4-98.4 %. The G+C content of the DNA of strain Fa-1(T) is 64.9 mol% (Tm). DNA-DNA hybridization values between strain Fa-1(T) and the most closely related members of the genus Halorubrum were below 51 %. On the basis of the data from this study, strain Fa-1(T) represents a novel species of the genus Halorubrum, for which the name Halorubrum litoreum sp. nov. is proposed. The type strain is Fa-1(T) (=CGMCC 1.5336(T) =JCM 13561(T)).
Cui
Heng-Lin
HL
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.|School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
Lin
Ze-Ying
ZY
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
Dong
Ying
Y
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
Zhou
Pei-Jin
PJ
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
Liu
Shuang-Jiang
SJ
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
D001482:Base Composition; D002681:China; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D006017:Glycolipids / Q000032:analysis; D006216:Halobacteriaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D006017:Glycolipids; D010743:Phospholipids; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D008274:Magnesium