Association of eNOS Glu298Asp gene polymorphism with essential hypertension in Asian Indians.
BACKGROUND
Several reports suggested association between the T allele of the endothelial nitric oxide synthase gene polymorphism (eNOS) Glu298Asp (G --> T at nucleotide 894, exon 7) with essential hypertension (EHT). Findings, however are not uniform. In this case-control study, we determined the prevalence and distribution of the above polymorphism and their relationship with the disease to elucidate the association of this polymorphism with the risk and pathophysiology of EHT in the Asian Indians and significance of T allele in this context.


METHODS
Two hundred normal controls and 226 patients with EHT from Delhi and surrounding areas were recruited for the investigation. Venous blood samples were used for genotyping. Genotyping was done by PCR-RFLP.


RESULTS
The data suggested higher prevalence of GT+TT genotypes in patients as compared to the controls and association of T allele with EHT [p<0.001, odds ratio at 95% CI, 2.10 (1.34-3.28)].


CONCLUSIONS
Asian Indians of this region with T allele may be at higher risk of EHT.
Srivastava
Kamna
K
Department of Biochemistry, India Institute of Medical Sciences, Ansari Nagar, New Delhi-110029, India.
Narang
Rajiv
R
Sreenivas
V
V
Das
Sabri
S
Das
Nibhriti
N
D000328:Adult; D000483:Alleles; D001224:Aspartic Acid / Q000235:genetics*; D005260:Female; D005838:Genotype; D018698:Glutamic Acid / Q000235:genetics*; D006801:Humans; D006973:Hypertension / Q000201:enzymology / Q000235:genetics*; D007194:India; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D011110:Polymorphism, Genetic
D001224:Aspartic Acid; D018698:Glutamic Acid; D052250:Nitric Oxide Synthase Type III