Specific capture of phosphopeptides by Zr4+-modified monolithic capillary column.
A method to prepare zirconium phosphate (ZrP)-modified monolithic capillary column for highly specific capture of phosphopeptides is presented. In this method, the phosphate monolithic capillary column was prepared by direct copolymerization of the functional monomer containing phosphate group (ethylene glycol methacrylate phosphate) and cross-linker (bis-acrylamide) in a ternary porogenic solvent. Copolymerization of cross-linker and functional monomer simplifies the procedure for the preparation of phosphate chromatographic media. After Zr4+ was immobilized, the ZrP-modified monolithic capillary column was evaluated by the analysis of standard phosphoproteins and the excellent selectivity of this approach was demonstrated by analyzing phosphopeptides in the digest mixture of beta-casein and BSA with molar ratio of 1:200.
Dong
Jing
J
National Chromatographic R&A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, China.
Zhou
Houjiang
H
Wu
Renan
R
Ye
Mingliang
M
Zou
Hanfa
H
D015394:Molecular Structure; D010316:Particle Size; D010748:Phosphopeptides / Q000032:analysis*; D016062:Porosity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods; D013499:Surface Properties; D013997:Time Factors; D015040:Zirconium / Q000737:chemistry*
D010748:Phosphopeptides; C027006:zirconium phosphate; D015040:Zirconium