title: Effects of nanoparticles on the adhesion and cell viability on astrocytes.
abstract: In recent years, both pharmaceutical companies and manufacturing industries have expressed heightened interest in the potential applications of magnetic nanoparticles for therapeutic and technological purposes. Specifically, pharmaceutical companies seek to employ magnetic nanoparticles as carriers to facilitate effective drug delivery, especially in areas of the brain. Manufacturing industries desire to use these nanoparticles as ferrofluids and in magnetic resonance imaging. However, data concerning the effects of magnetic nanoparticles on the nervous system is limited. This study tested the hypotheses that nanoparticles can (1) inhibit adherence of astrocytes to culture plates and (2) cause cytotoxicity or termination of growth, both end points representing surrogate markers of neurotoxicity. Using light microscopy, changes in plating patterns were determined by visual assessment. Cell counting 4 days after plating revealed a significant decrease in the number of viable astrocytes in nanoparticle treated groups (p < 0.0001). To determine the cytotoxic effects of nanoparticles, astrocytes were allowed to adhere to culture plates and grow to maturity for 3 weeks before treatment. Membrane integrity and mitochondrial function were measured using colorimetric analysis lactate dehydrogenase (LDH) and 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTS), respectively. Treatment with nanoparticles did not significantly alter astrocytic LDH release (p > 0.05) in the control group (100% +/- 1.56) vs the group receiving treatment (97.18% +/- 2.03). However, a significant increase in MTS activity (p < 0.05) between the control (100% +/- 3.65) and treated groups (112.8% +/- 3.23) was observed, suggesting astrocytic mitochondrial uncoupling by nanoparticles. These data suggest that nanoparticles impede the attachment of astrocytes to the substratum. However, once astrocytes attach to the substratum and grow to confluence, nanoparticles may cause mitochondrial stress.
authors.affiliation: Center for Molecular Neuroscience, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
authors.forename: Catherine
authors.initials: C
authors.lastname: Au
authors.forename: Lysette
authors.initials: L
authors.lastname: Mutkus
authors.forename: Allison
authors.initials: A
authors.lastname: Dobson
authors.forename: Judy
authors.initials: J
authors.lastname: Riffle
authors.forename: Jennifer
authors.initials: J
authors.lastname: Lalli
authors.forename: Michael
authors.initials: M
authors.lastname: Aschner
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology*; D008280:Magnetics; D008928:Mitochondria / Q000502:physiology; D053758:Nanoparticles; D051381:Rats; D017207:Rats, Sprague-Dawley