Removal of the glycosylation of prion protein provokes apoptosis in SF126.
Although the function of cellular prion protein (PrPc) and the pathogenesis of prion diseases have been widely described, the mechanisms are not fully clarified. In this study, increases of the portion of non-glycosylated prion protein deposited in the hamster brains infected with scrapie strain 263K were described. To elucidate the pathological role of glycosylation profile of PrP, wild type human PrP (HuPrP) and two genetic engineering generated non-glycosylated PrP mutants (N181Q/N197Q and T183A/T199A) were transiently expressed in human astrocytoma cell line SF126. The results revealed that expressions of non-glycosylated PrP induced significantly more apoptosis cells than that of wild type PrP. It illustrated that Bcl-2 proteins might be involved in the apoptosis pathway of non-glycosylated PrPs. Our data highlights that removal of glycosylation of prion protein provokes cells apoptosis.
Chen
Lan
L
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Science, Chinese Academy of Medical Sciences and Peking Union Medical College, Dong Dan San Tiao 5, Beijing 100005, Peopleos Republic of China.
Yang
Yang
Y
Han
Jun
J
Zhang
Bao-Yun
BY
Zhao
Lin
L
Nie
Kai
K
Wang
Xiao-Fan
XF
Li
Feng
F
Gao
Chen
C
Dong
Xiao-Ping
XP
Xu
Cai-Min
CM
D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D006224:Cricetinae; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006031:Glycosylation; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D009154:Mutation; D009711:Nucleotides / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D018620:PrPSc Proteins / Q000378:metabolism; D011328:Prions / Q000235:genetics / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D014162:Transfection; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D009711:Nucleotides; D018620:PrPSc Proteins; D011328:Prions; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein