Optical and electrochemical detection of saccharides with poly(aniline-co-3-aminobenzeneboronic acid) prepared from enzymatic polymerization.
Boronic acid-based sensors for saccharides have been developed via biocatalysis. The self-doped copolymer of poly(aniline-co-3-aminobenzeneboronic acid) [poly(aniline-co-AB)], with various mole ratios of two components, was synthesized by oxidative enzymatic polymerization using a natural biocatalyst such as horseradish peroxidase together with an anionic polyelectrolyte template (sulfonated polystyrene) under mild conditions (pH 4.5). Poly(aniline-co-AB), having an aniline boronic acid-to-aniline ratio of 1:2 on average, gave rise to a green doped polymer with absorption maxima at 745 nm. The potentiometric detection of saccharides using poly(aniline-co-AB) is presented. Characteristics of both transient and steady-state response associated with the complex formation of poly(aniline-co-AB) with various saccharides were monitored by UV-vis spectroscopy and cyclic voltammetry (CV). The results obtained from UV-vis spectroscopy and CV show that the sensitivity of enzymatically synthesized water-soluble poly(aniline-co-AB) for various saccharides was improved significantly compared to the chemically synthesized counterpart. A possible mechanism for the sensitive detection of sugar molecules by boronic acid is proposed on the basis of UV-vis and IR spectrophotometry, and four-point probe conductivity measurements.
Huh
Pilho
P
Center for Advanced Materials, Polymer Science Program, Departments of Chemistry and Physics, University of Massachusetts, Lowell, MA 01854,USA.
Kim
Seong-Cheol
SC
Kim
Younghoon
Y
Wang
Yanping
Y
Singh
Jagdeep
J
Kumar
Jayant
J
Samuelson
Lynne A
LA
Kim
Bong-Soo
BS
Jo
Nam-Ju
NJ
Lee
Jang-Oo
JO
D000814:Aniline Compounds / Q000737:chemistry* / Q000378:metabolism*; D001897:Boronic Acids / Q000737:chemistry* / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry*; D004563:Electrochemistry; D006735:Horseradish Peroxidase / Q000378:metabolism*; D015394:Molecular Structure; D013053:Spectrophotometry
D000814:Aniline Compounds; D001897:Boronic Acids; D002241:Carbohydrates; C416807:polyaniline; C028592:3-aminobenzeneboronic acid; D006735:Horseradish Peroxidase