Apolipoprotein E receptors and amyloid expression are modulated in an apolipoprotein E-dependent fashion in response to hippocampal deafferentation in rodent.
The entorhinal cortex lesion paradigm is a widely accepted and efficient method to provoke reactive synaptogenesis and terminal remodeling in the adult CNS. This approach has been used successfully to contrast the profile of reactivity from various proteins associated with Alzheimer's disease pathophysiology in wild-type and apolipoprotein E (apoE)-deficient (APOE ko) mice. Results indicate that the production of the beta-amyloid 1-40 peptide (A beta 40) is increased in response to neuronal injury, with a timing that is different between wild-type and APOE ko animals. Moreover, we report that baseline levels of the A beta 40 peptide are significantly higher in the APOE ko mice. The expression of the apolipoprotein E receptor type 2 (apoER2) is also modulated by the deafferentation process in the hippocampus, but only in APOE ko mice. These results provide novel insights as to the molecular mechanisms responsible for the poor plastic response reported in apoE4-expressing and apoE deficient mice in response to hippocampal injury.
Petit-Turcotte
C
C
McGill Center for Studies in Aging, 6825 LaSalle Boulevard, Verdun, Quebec, Canada H4H 1R3.
Aumont
N
N
Blain
J-F
JF
Poirier
J
J
D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency / Q000502:physiology*; D001930:Brain Injuries / Q000209:etiology / Q000473:pathology*; D004572:Electrolysis / Q000009:adverse effects; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007839:Functional Laterality / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology*; D006624:Hippocampus / Q000293:injuries / Q000378:metabolism*; D026502:LDL-Receptor Related Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010446:Peptide Fragments / Q000378:metabolism*; D018110:Receptors, Lipoprotein / Q000378:metabolism*; D013997:Time Factors
D016229:Amyloid beta-Peptides; D001057:Apolipoproteins E; D026502:LDL-Receptor Related Proteins; D010446:Peptide Fragments; D018110:Receptors, Lipoprotein; C075222:amyloid beta-protein (1-42); C098773:low density lipoprotein receptor-related protein 8