title: A new polymerase chain reaction/restriction fragment length polymorphism protocol for Plasmodium vivax circumsporozoite protein genotype (VK210, VK247, and P. vivax-like) determination.
abstract: For the molecular diagnosis of Plasmodium vivax variants (VK210, VK247, and P. vivax-like) using DNA amplification procedures in the laboratory, the choice of rapid and inexpensive identification products of the 3 different genotypes is an important prerequisite. We report here the standardization of a new polymerase chain reaction/restriction fragment length polymorphism technique to identify the 3 described P. vivax circumsporozoite protein (CSP) variants using amplification of the central immunodominant region of the CSP gene of this protozoan. The simplicity, specificity, and sensitivity of the system described here is important to determine the prevalence and the distribution of infection with these P. vivax genotypes in endemic and nonendemic malaria areas, enabling a better understanding of their phylogeny.
authors.affiliation: Universidade Estadual Paulista Júlio de Mesquita Filho, 15054-000 São José do Rio Preto, São Paulo State, Brazil.
authors.forename: Renata Tomé
authors.initials: RT
authors.lastname: Alves
authors.forename: Marinete Marins
authors.initials: MM
authors.lastname: Póvoa
authors.forename: Ira F
authors.initials: IF
authors.lastname: Goldman
authors.forename: Carlos Eugênio
authors.initials: CE
authors.lastname: Cavasini
authors.forename: Andréa Regina Baptista
authors.initials: AR
authors.lastname: Rossit
authors.forename: Ricardo Luiz Dantas
authors.initials: RL
authors.lastname: Machado
mesh_terms: D000818:Animals; D005838:Genotype; D006801:Humans; D016780:Malaria, Vivax / Q000469:parasitology*; D010274:Parasitology / Q000379:methods; D010966:Plasmodium vivax / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D015800:Protozoan Proteins / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan