title: Benign prostatic hyperplasia (BPH) epithelial cell line BPH-1 induces aromatase expression in prostatic stromal cells via prostaglandin E2.
abstract: Estradiol (E2) level in stroma of benign prostatic hyperplasia (BPH) increases with age, and this increase was associated with an elevated expression of aromatase in prostatic stromal cells (PrSCs). Here, we showed that conditioned medium (CM) of BPH-1 (a benign hyperplastic prostatic epithelial cell line), but not of prostate cancer cell lines (LNCaP, DU-145, and PC-3), stimulates aromatase expression in PrSCs. Cyclooxygenase-2 (COX-2) mRNA level in BPH-1, as well as prostaglandin E2 (PGE2) concentration in BPH-1 CM, was significantly higher than that of prostate cancer cell lines. CM of BPH-1 treated with NS-398 (a specific inhibitor of COX-2) failed to stimulate aromatase expression in PrSCs. And PGE2 can stimulate aromatase expression in PrSCs. Our data suggested that BPH-1 induced aromatase expression in PrSCs through the production of PGE2 in a paracrine mechanism.
authors.affiliation: Bioactive Materials Key Lab of Ministry of Education, Institute for Molecular, Biology, Nankai University, Tianjin 300071, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Wu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Linfeng
authors.initials: L
authors.lastname: Chen
authors.forename: Jiandang
authors.initials: J
authors.lastname: Shi
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Miao
authors.forename: Helmut
authors.initials: H
authors.lastname: Klocker
authors.forename: Irwin
authors.initials: I
authors.lastname: Park
authors.forename: Chung
authors.initials: C
authors.lastname: Lee
authors.forename: Ju
authors.initials: J
authors.lastname: Zhang
mesh_terms: D001141:Aromatase / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western / Q000379:methods; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D015232:Dinoprostone / Q000378:metabolism*; D004847:Epithelial Cells; D015870:Gene Expression; D006801:Humans; D008297:Male; D019899:Paracrine Communication / Q000502:physiology*; D011467:Prostate / Q000201:enzymology*; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000201:enzymology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017154:Stromal Cells / Q000201:enzymology*
chemical_list: D017077:Culture Media, Conditioned; D001141:Aromatase; D015232:Dinoprostone