A knowledge-based forcefield for protein-protein interface design.
A distance-dependent knowledge-based potential for protein-protein interactions is derived and tested for application in protein design. Information on residue type specific C(alpha) and C(beta) pair distances is extracted from complex crystal structures in the Protein Data Bank and used in the form of radial distribution functions. The use of only backbone and C(beta) position information allows generation of relative protein-protein orientation poses with minimal sidechain information. Further coarse-graining can be done simply in the same theoretical framework to give potentials for residues of known type interacting with unknown type, as in a one-sided interface design problem. Both interface design via pose generation followed by sidechain repacking and localized protein-protein docking tests are performed on 39 nonredundant antibody-antigen complexes for which crystal structures are available. As reference, Lennard-Jones potentials, unspecific for residue type and biasing toward varying degrees of residue pair separation are used as controls. For interface design, the knowledge-based potentials give the best combination of consistently designable poses, low RMSD to the known structure, and more tightly bound interfaces with no added computational cost. 77% of the poses could be designed to give complexes with negative free energies of binding. Generally, larger interface separation promotes designability, but weakens the binding of the resulting designs. A localized docking test shows that the knowledge-based nature of the potentials improves performance and compares respectably with more sophisticated all-atoms potentials.
Clark
Louis A
LA
Biogen Idec Inc., Protein Engineering Group, Cambridge, Massachusetts 02142, USA. louie@alumni.northwestern.edu
van Vlijmen
Herman W T
HW
D000936:Antigen-Antibody Complex / Q000737:chemistry; D001665:Binding Sites; D019295:Computational Biology; D051188:Knowledge Bases; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*
D000936:Antigen-Antibody Complex; D011506:Proteins