Lanthanide-containing polymer nanoparticles for biological tagging applications: nonspecific endocytosis and cell adhesion.
We describe the synthesis and characterization of element-encoded polystyrene nanoparticles with diameters on the order of 100 nm and a narrow size distribution. Individual particles contain ca. 10(3) chelated lanthanide ions, of either a single element or a mixture of elements. These particles were effectively internalized by nonspecific endocytosis into three cell lines associated with human leukemia. Using an assay based upon ICP-MS detection, we could monitor quantitatively cell adhesion induced by cell differentiation of THP-1 cells in response to phorbol ester stimulation (PMA) in single cell type or mixed cultures.
Vancaeyzeele
Cédric
C
Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada.
Ornatsky
Olga
O
Baranov
Vladimir
V
Shen
Lei
L
Abdelrahman
Ahmed
A
Winnik
Mitchell A
MA
D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D004705:Endocytosis / Q000187:drug effects*; D006801:Humans; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010703:Phorbol Esters / Q000494:pharmacology; D011137:Polystyrenes / Q000138:chemical synthesis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013329:Structure-Activity Relationship
D028581:Lanthanoid Series Elements; D010703:Phorbol Esters; D011137:Polystyrenes