title: Preparation of tiny biodegradable capsules using electrocapillary emulsification.
abstract: Conditions for preparing tiny biodegradable capsules were examined using electrocapillary emulsification that allows one to prepare monodisperse emulsions with ease by applying a DC potential between oil and water phases without mechanical agitation. The results obtained showed that a 1 : 4 mixture of polysorbate 80 (TO-10), a hydrophilic non-ionic surfactant, and sorbitan monooleate (SO-10), an oleophilic surfactant, is appropriate as the surfactant to be added to oil phase, cyclohexane is acceptable as the oil phase and 1000 V is optimum as the DC potential to be applied. The capsules prepared had sizes ranging from 100-300 nm and a surface roughness of approximately 10 nm and degraded in model intestinal juice more easily and rapidly than in model gastric juice. In addition, the capsules containing lactoferrin, an anti-carcinogenic protein, were found to keep 12.5% of the protein used in encapsulation without losing its activity.
authors.affiliation: Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba, Japan. abemasa@rs.noda.tus.ac.jp
authors.forename: Masahiko
authors.initials: M
authors.lastname: Abe
authors.forename: Ai
authors.initials: A
authors.lastname: Nakayama
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Kondo
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Morishita
authors.forename: Koji
authors.initials: K
authors.lastname: Tsuchiya
authors.forename: Shigenori
authors.initials: S
authors.lastname: Utsumi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ohkubo
authors.forename: Hideki
authors.initials: H
authors.lastname: Sakai
mesh_terms: D003510:Cyclohexanes / Q000737:chemistry*; D004563:Electrochemistry; D004655:Emulsions / Q000737:chemistry; D005750:Gastric Juice / Q000737:chemistry; D006601:Hexoses / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011136:Polysorbates / Q000737:chemistry*
chemical_list: D003510:Cyclohexanes; D004655:Emulsions; D006601:Hexoses; D053769:Nanocapsules; D011136:Polysorbates; C018665:sorbitan monooleate; C506365:Cyclohexane