title: Endothelial NO/cGMP-dependent vascular relaxation of cornuside isolated from the fruit of Cornus officinalis.
abstract: Cornuside is a bisiridoid glucoside compound isolated from the fruit of CORNUS OFFICINALIS Sieb. et Zucc. (Cornaceae). In the present study, we investigated the effect of cornuside on vascular tone in rat aortic tissue. Cornuside induced a concentration-dependent relaxation of the phenylephrine-precontracted rat aorta, which was abolished by removal of the endothelial layer. Pretreatment of the aortic tissues with either N(G)-nitro- L-arginine methyl ester (L-NAME) or 1 H- -oxadiazole-[4,3-alpha]-quinoxalin-1-one (ODQ) completely inhibited the relaxation induced by cornuside. However, the relaxant effect of cornuside was not blocked by pretreatment with verapamil, diltiazem, tetraethylammonium (TEA), glibenclamide, indomethacin, atropine, or propranolol. In addition, incubation of human umbilical vein endothelial cells (HUVECs) with cornuside increased the production of cGMP in a dose-dependent manner, but this effect was blocked by pretreatment with L-NAME and ODQ, respectively. Taken together, the present study suggests that cornuside dilates vascular smooth muscle via endothelium-dependent nitric oxide (NO)/cGMP signaling.
authors.affiliation: Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, Republic of Korea.
authors.forename: Dae Gill
authors.initials: DG
authors.lastname: Kang
authors.forename: Deok Ho
authors.initials: DH
authors.lastname: Choi
authors.forename: Jun Kyoung
authors.initials: JK
authors.lastname: Lee
authors.forename: Yun Jeong
authors.initials: YJ
authors.lastname: Lee
authors.forename: Mi Kyoung
authors.initials: MK
authors.lastname: Moon
authors.forename: Sun Nye
authors.initials: SN
authors.lastname: Yang
authors.forename: Tae Oh
authors.initials: TO
authors.lastname: Kwon
authors.forename: Ji Woong
authors.initials: JW
authors.lastname: Kwon
authors.forename: Jin Sook
authors.initials: JS
authors.lastname: Kim
authors.forename: Ho Sub
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects; D029768:Cornus / Q000737:chemistry*; D006152:Cyclic GMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000502:physiology; D005638:Fruit / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism*; D011714:Pyrans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014664:Vasodilation / Q000187:drug effects*
chemical_list: D005960:Glucosides; D011714:Pyrans; C080726:cornuside; D009569:Nitric Oxide; D006152:Cyclic GMP