Characterisation of 3-methylorcinaldehyde synthase (MOS) in Acremonium strictum: first observation of a reductive release mechanism during polyketide biosynthesis.
Isolation and sequencing of a PKS gene isolated from xenovulene-producing cultures of Acremonium strictum indicated the presence of NT-, KS-, AT-, PT-, C-MeT- and R-domains; heterologous expression in Aspergillus oryzae resulted in the production of 3-methylorcinaldehyde, demonstrating the role of the terminal reductase domain in product release.
Bailey
Andrew M
AM
School of Biological Sciences, University of Bristol, Woodland Road, Bristol, UK BS8 1UG.
Cox
Russell J
RJ
Harley
Kate
K
Lazarus
Colin M
CM
Simpson
Thomas J
TJ
Skellam
Elizabeth
E
D000164:Acremonium / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000447:Aldehydes / Q000737:chemistry* / Q000378:metabolism*; D018942:Macrolides / Q000737:chemistry* / Q000378:metabolism*; D008745:Methylation; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010802:Phylogeny
D000447:Aldehydes; D018942:Macrolides