Environmental factors of celiac disease: cytotoxicity of hulled wheat species Triticum monococcum, T. turgidum ssp. dicoccum and T. aestivum ssp. spelta.
OBJECTIVE
In the present paper, the toxicity of prolamines derived from three cereals with a different genome was investigated in human colon cancer Caco-2/TC7 and human myelogenous leukemia K562(S) cells. The purpose of this study was to investigate if species from ancient wheat could be considered as healthy food crops devoid or poor in cytotoxic prolamines for celiac disease.


METHODS
Cytotoxicity was measured in terms of inhibition of cell growth, activation of apoptosis, release of nitric oxide (NO), detection of tissue transglutaminase (TG II) and alteration of transepithelial electrical resistance (TEER) on Caco-2/Tc7 and K562 (S) cell agglutination. Peptic-tryptic (PT) digest from bread wheat (T. aestivum S. Pastore) was used as a positive control.


RESULTS
PT digests of prolamins from spelt wheat (T. aestivum ssp. spelta) were found to exert toxic effects on Caco-2/TC7 cells and to agglutinate K562(S) cells. Increased amounts of NO and TG II expression were observed in Caco-2/TC7 cells exposed to 1 mg/mL of spelt prolamins, suggesting that spelt wheat can induce cellular mechanisms implicated in the pathogenesis of celiac disease. By contrast, the PT digests from monoccum wheat (Triticum monococcum) and farro wheat (T. turgidum ssp. dicoccum) did not exhibit any negative effects on Caco-2/TC7 and K562(S) cells.


CONCLUSIONS
The results have shown a constant and significant toxic effect of spelt wheat which is not shared by the two other ancient cereals. Future studies on celiac intestinal organ cultures are needed to increase the prospects of breeding programs aimed at developing wheat cultivars potentially tolerated by most celiac patients.
Vincentini
Olimpia
O
Division of Food Science, Human Nutrition and Health, National Institute of Health, Rome, Italy.
Maialetti
Francesca
F
Gazza
Laura
L
Silano
Marco
M
Dessi
Mariarita
M
De Vincenzi
Massimo
M
Pogna
Norberto Edgardo
NE
D000371:Agglutination / Q000187:drug effects; D000372:Agglutination Tests; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D018938:Caco-2 Cells; D020169:Caspases / Q000378:metabolism; D002446:Celiac Disease / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D017097:Electric Impedance; D004789:Enzyme Activation; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D020014:K562 Cells; D009569:Nitric Oxide / Q000378:metabolism; D010539:Permeability / Q000187:drug effects; D010940:Plant Proteins / Q000302:isolation & purification / Q000633:toxicity*; D055315:Prolamins; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D013997:Time Factors; D011503:Transglutaminases / Q000378:metabolism; D014908:Triticum / Q000737:chemistry
D010940:Plant Proteins; D055315:Prolamins; D009569:Nitric Oxide; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D020169:Caspases; D019204:GTP-Binding Proteins