Cholesterol biosensor based on electrophoretically deposited conducting polymer film derived from nano-structured polyaniline colloidal suspension.
Cholesterol oxidase (ChOx) has been covalently immobilized onto electrophoretically deposited conducting polymer film (on indium-tin-oxide (ITO) glass plate) derived from nano-structured polyaniline (PANI) colloidal suspension using N-ethyl-N'-(3-dimethylaminopropyl) corbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry. These PANI/ITO and ChOx/PANI/ITO electrodes have been characterized using ultraviolet-visible (UV-vis), Fourier transform-infrared (FT-IR), scanning electron microscopy (SEM), and impedance spectroscopy techniques, respectively. These ChOx/PANI/ITO bio-electrodes exhibit linearity from 25 to 400 mg dL(-1) of cholesterol, detection limit as 25 mg dL(-1) and sensitivity as 7.76x10(-5) Abs(mg/dL)(-1). The value of the apparent Michaelis-Menten constant (Km(app)) calculated from amperometric response studies has been found to be 0.62 mM for ChOx/PANI/ITO bio-electrode.
Dhand
Chetna
C
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K.S. Krishnan Marg, New Delhi 110012, India.
Singh
S P
SP
Arya
Sunil K
SK
Datta
Monika
M
Malhotra
B D
BD
D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002784:Cholesterol / Q000032:analysis* / Q000378:metabolism*; D002789:Cholesterol Oxidase / Q000378:metabolism; D003102:Colloids / Q000737:chemistry; D017097:Electric Impedance; D004563:Electrochemistry; D004566:Electrodes; D004586:Electrophoresis / Q000379:methods*; D006735:Horseradish Peroxidase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D019265:Spectroscopy, Near-Infrared; D013535:Suspensions / Q000737:chemistry; D013696:Temperature
D000814:Aniline Compounds; D003102:Colloids; D011108:Polymers; D013535:Suspensions; C416807:polyaniline; D002784:Cholesterol; D002789:Cholesterol Oxidase; D006735:Horseradish Peroxidase