Using lasers to probe the transient light absorption by proteorhodopsin in marine bacterioplankton.
We constructed an experimental apparatus that used lasers to provide the probe beams for measuring the transient absorption kinetics of bacterioplankton that contain proteorhodopsin, a microbial protein that binds retinal and is analogous to animal rhodopsin. With this approach we were able to observe photocycles characteristic of functioning retinylidene ion pumps. Using light from lasers instead of broadband sources as transmittance probe beams can be advantageous when examining optically dense, highly scattering samples such as concentrated microbial cultures. Such a laser-based approach may prove useful in shipboard studies for identifying proteorhodopsin in whole cell suspensions concentrated from seawater.
Desiderio
Russell A
RA
College of Oceanic & Atmospheric Sciences, Oregon State University, Corvallis, Oregon 97331, USA. desi@coas.oregonstate.edu
Laney
Samuel R
SR
Letelier
Ricardo M
RM
Giovannoni
Stephen J
SJ
D000042:Absorption; D000818:Animals; D004867:Equipment Design; D007477:Ions; D007700:Kinetics; D007834:Lasers; D008027:Light; D008956:Models, Chemical; D010933:Plankton / Q000378:metabolism*; D012176:Retinoids / Q000737:chemistry; D012243:Rhodopsin / Q000737:chemistry*; D025604:Rhodopsins, Microbial; D013053:Spectrophotometry / Q000379:methods; D013057:Spectrum Analysis
D007477:Ions; D012176:Retinoids; D025604:Rhodopsins, Microbial; C413548:proteorhodopsin; C121835:retinylidene chromophore; D012243:Rhodopsin