Fluorescent probe ABM for screening gastrointestinal patient's immune state.
The fluorescent probe-aminoderivative of benzanthrone, ABM (developed at Riga Technical University, Riga, Latvia) was used to characterize the membranes of lymphocytes of cancer patients: 46 patients with gastrointestinal diseases, 13 patients having different primary localizations with massive metastases and intoxication. Patients were divided into three groups: (1) with decreased fluorescence intensity, (2) normal fluorescence intensity, (3) increased fluorescence intensity. The lymphocytes distribution among subsets differed between groups, in correspondence to the level of fluorescence intensity. Surgical treatment affected the main immunological parameters and elevated the functional activity of lymphocytes. In the advanced tumors group, fluorescence intensity correlates with the survival rate. Results suggest that determination of lymphocytes functional activity by ABM can aid evaluation of the immune status in cancer patients.
Kalnina
Inta
I
Department of Chemistry, Daugavpils University, Daugavpils, Latvia.
Klimkane
Laura
L
Kirilova
Elena
E
Toma
Maija Malda
MM
Kizane
Gunta
G
Meirovics
Imants
I
D001551:Benz(a)Anthracenes / Q000737:chemistry; D002462:Cell Membrane / Q000276:immunology; D005456:Fluorescent Dyes / Q000737:chemistry; D005770:Gastrointestinal Neoplasms / Q000276:immunology* / Q000628:therapy; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D013050:Spectrometry, Fluorescence
D001551:Benz(a)Anthracenes; D005456:Fluorescent Dyes