title: A large-scale method for the selective depletion of alphabeta T lymphocytes from PBSC for allogeneic transplantation.
abstract: BACKGROUND
  We sought to develop a method for the clinical large-scale depletion of alphabeta T lymphocytes from mobilized peripheral stem cells, which would allow the allogeneic transplantation of a graft enriched for stem cells, natural killer (NK) cells and gammadelta T lymphocytes.
  
  
  METHODS
  Therefore, we obtained mononuclear cells from either mobilized or non-mobilized healthy adult volunteer donors and incubated the cells with a biotinylated anti-alphabeta T-cell Ab and subsequently with an anti-biotin Ab conjugated with magnetic microbeads. The depletion was then performed using a CliniMACS device.
  
  
  RESULTS
  The median T-cell depletion was 3.9 log (range 3.5-4.1 log). The recovery of the gammadelta and NK cells was 92% and 80%, respectively. The recovery of CD34+ stem cells from the mobilized donors was 66%.
  
  
  CONCLUSIONS
  This method had no negative influence on the in vitro colony formation of stem cells, and transplantation of alphabeta-depleted cells into NOD-SCID IL-2 common gamma chain knockout (NOD-scid IL2r (null)) mice resulted in a rapid engraftment of human myeloid and lymphoid cells. This method will allow large-scale depletion of alphabeta T cells from mobilized peripheral blood in clinical trials.
authors.affiliation: Division of Stem Cell Transplantation, Department of Hematology-Oncology, St Jude Children's Research Hospital, Memphis, Tennessee, USA.
authors.forename: S
authors.initials: S
authors.lastname: Chaleff
authors.forename: M
authors.initials: M
authors.lastname: Otto
authors.forename: R C
authors.initials: RC
authors.lastname: Barfield
authors.forename: T
authors.initials: T
authors.lastname: Leimig
authors.forename: R
authors.initials: R
authors.lastname: Iyengar
authors.forename: J
authors.initials: J
authors.lastname: Martin
authors.forename: M
authors.initials: M
authors.lastname: Holiday
authors.forename: J
authors.initials: J
authors.lastname: Houston
authors.forename: T
authors.initials: T
authors.lastname: Geiger
authors.forename: V
authors.initials: V
authors.lastname: Huppert
authors.forename: R
authors.initials: R
authors.lastname: Handgretinger
mesh_terms: D000328:Adult; D000818:Animals; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D016130:Immunophenotyping; D008212:Lymphocyte Depletion / Q000379:methods*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D036102:Peripheral Blood Stem Cell Transplantation / Q000379:methods; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014184:Transplantation, Homologous
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta