The anti-apoptosis effects of daidzein in the brain of D-galactose treated mice.
The purpose of this study was to explore the neuroprotective effects of daidzein on the apoptotic pathway in the hippocampus and cortex of D-galactose treated mice. For this purpose we have examined the expression of bcl-2 mRNA, bax mRNA and caspase-3 in the hippocampus and cortex of D-galactose-treated mice after fed with 10 or 5 mg/kg of daidzein. The results of in situ hybridization experiments indicate that daidzein could help increase the transcriptions of bcl-2 and decrease the transcriptions of bax in those brain regions of D-galactose-treated mice. Furthermore, immunohistochemical studies showed that daidzein could reduce the expression of caspase-3 in both brain regions. These results suggest that daidzein in soybean can inhibit the D-gal induced apoptosis via Bcl-2/Bax apoptotic pathway and be a potential medical candidate for neurodegeneration therapy.
Mao
Zhen
Z
Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Xuzhou Normal University, Xuzhou 221116, Jiangsu Province, PR China. zhenmao80@yahoo.com.cn
Zheng
Yuan-lin
YL
Zhang
Yan-qiu
YQ
Han
Bao-ping
BP
Zhu
Xiao-wan
XW
Chang
Qing
Q
Hu
Xiang-bin
XB
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001921:Brain / Q000166:cytology* / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D004958:Estradiol / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D005690:Galactose / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D017403:In Situ Hybridization; D007529:Isoflavones / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D048789:Phytoestrogens / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
D007529:Isoflavones; D048789:Phytoestrogens; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D004958:Estradiol; C004742:daidzein; D053148:Caspase 3; D005690:Galactose