Expression of glycerol 3-phosphate dehydrogenase gene (CvGPD1) in salt-tolerant yeast Candida versatilis is stimulated by high concentrations of NaCl.
We cloned the glycerol 3-phosphate dehydrogenase (GPDH) gene (CvGPD1) from salt-tolerant yeast Candida versatilis. When CvGPD1 was expressed in glycerol synthesis-deficient Saccharomyces cerevisiae cells, the salt tolerance of the recombinant strain was enhanced, and NADP(+)-dependent GPDH (EC 1.1.1.94), Cvgpd1p synthesis and recovery of glycerol synthesis were confirmed. The transcription of CvGPD1 in C. versatilis cells was stimulated by high concentrations of NaCl. The relationship between expression of CvGPD1 and growth of C. versatilis cells in the mash of Japanese seasonings (miso- and shoyu-moromi) is also discussed.
Watanabe
Yasuo
Y
Department of Biological Resources, National University Corporation Ehime University, Matsuyama, Ehime, Japan. watanabe@agr.ehime-u.ac.jp
Nagayama
Kisako
K
Tamai
Youichi
Y
D000595:Amino Acid Sequence; D002175:Candida / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+) / Q000235:genetics*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects
D012965:Sodium Chloride; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+)