title: Distribution of prion protein genotypes in breeds of sheep in New Zealand.
abstract: OBJECTIVE
  To use an established high through-put genotyping procedure to gain an estimate of the frequency of alleles of the prion protein (PrP) gene in some common sheep breeds in New Zealand.
  
  
  METHODS
  Using a genotyping procedure based on matrix-assisted laser desorption ionisation-time of flight (MALDI-TOF), DNA samples from 3,024 sheep from New Zealand, including breeds such as Romney, Texel, Coopworth, Merino and mixed breed, were isolated, genotyped and the results analysed.
  
  
  RESULTS
  The 15 scrapie genotypes commonly reported, and derived from the five commonly reported allelic variants (ARR, ARQ, AHQ, ARH and VRQ), were all observed in the samples analysed. The estimates were indicative of the frequencies in the population of alleles present in breeds of sheep in New Zealand. There was a significant difference between the frequencies of alleles between breeds, but the ARQ, followed by the ARR allele, were, except in Carwell sheep, the most common alleles present.
  
  
  CONCLUSIONS
  This study gave an indication of the percentages of PrP gene alleles in sheep in New Zealand, including data previously unreported from breeds in this country. It is of interest because of the relatively large size of the sheep population in New Zealand compared with many countries, and it provides some useful information on the genetic susceptibility or resistance of the sheep population in New Zealand to scrapie. The frequencies of the alleles can be different for an individual breed compared between countries.
authors.affiliation: Department of Economics, University of Otago, Dunedin, New Zealand.
authors.forename: M A
authors.initials: MA
authors.lastname: Lee
authors.forename: T R
authors.initials: TR
authors.lastname: Manley
authors.forename: B C
authors.initials: BC
authors.lastname: Glass
authors.forename: R M
authors.initials: RM
authors.lastname: Anderson
authors.forename: S J
authors.initials: SJ
authors.lastname: Wilson
authors.forename: J S
authors.initials: JS
authors.lastname: O'keefe
authors.forename: D J
authors.initials: DJ
authors.lastname: Tisdall
authors.forename: J C
authors.initials: JC
authors.lastname: McEwan
authors.forename: S H
authors.initials: SH
authors.lastname: Phua
authors.forename: M L
authors.initials: ML
authors.lastname: Tate
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis; D017931:DNA Primers; D005260:Female; D005838:Genotype; D009520:New Zealand / Q000453:epidemiology; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000662:veterinary; D018620:PrPSc Proteins / Q000235:genetics / Q000302:isolation & purification*; D012608:Scrapie / Q000453:epidemiology* / Q000209:etiology / Q000382:microbiology*; D012756:Sheep
chemical_list: D017931:DNA Primers; D018620:PrPSc Proteins; D004247:DNA