Production of antibodies for selective detection of malachite green and the related triphenylmethane dyes in fish and fishpond water.
This study provides a practical method for production of the antibodies against malachite green (MG) and its primary metabolite leucomalachite green (LMG). Two ELISA kits are constructed with the MG and LMG antibodies for detection of the residual MG and LMG in fish muscle and fishpond water. The detection limit is established at the level of 0.05 microg/L for both MG and LMG. Our ELISA kits show the advantages of good specificity, high sensitivity, and convenience in rapid screening of MG and LMG residues. The sample of fishpond water, without extraction or prior preparation, is directly assayed by the ELISA kit. More then 80 fish samples can be simultaneously tested in a kit. The toxic crystal violet and its metabolite leucocrystal violet of illegal use in aquaculture are detected by our prepared MG and LMG antibodies, whereas the antibodies do not cross-react with common antibiotics, sulfonamides, and benzene derivatives.
Yang
Mei-Chun
MC
GlycoNex Inc., Taipei County, 221, Taiwan.
Fang
Jim-Min
JM
Kuo
Tzong-Fu
TF
Wang
Da-Ming
DM
Huang
Yi-Lin
YL
Liu
Liang-Yirn
LY
Chen
Pen-Heng
PH
Chang
Tong-Hsuan
TH
D000814:Aniline Compounds / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D017756:Aquaculture; D004797:Enzyme-Linked Immunosorbent Assay; D005399:Fishes; D005659:Fungicides, Industrial / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D012394:Rosaniline Dyes / Q000032:analysis* / Q000276:immunology; D014867:Water / Q000032:analysis*
D000814:Aniline Compounds; D000906:Antibodies; D005659:Fungicides, Industrial; D012394:Rosaniline Dyes; D014867:Water; C005095:malachite green; C015320:leucomalachite green