Plant organelle proteomics.
It is important for cell biologists to know the subcellular localization of proteins to understand fully the functions of organelles and the compartmentation of plant metabolism. The accurate description of an organelle proteome requires the ability to identify genuine protein residents. Such accurate assignment is difficult in situations where a pure homogeneous preparation of the organelle cannot be achieved. Practical limitations in both organelle isolation and also analysis of low abundance proteins have resulted in limited datasets from high throughput proteomics approaches. Here, we discuss some examples of quantitative proteomic methods and their use to study plant organelle proteomes, with particular reference to methods designed to give unequivocal assignments to organelles.
Lilley
Kathryn S
KS
Cambridge Centre for Proteomics, University of Cambridge, Cambridge CB2 1QR, United Kingdom. k.s.lilley@bioc.cam.ac.uk
Dupree
Paul
P
D017360:Arabidopsis / Q000378:metabolism*; D002451:Cell Compartmentation; D015388:Organelles / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics / Q000379:methods
D010940:Plant Proteins; D020543:Proteome