[Effect of hemoperfusion treated plasma from patients with chronic severe hepatitis on the activity and expression of CYP4503A in C3A cells].
OBJECTIVE
To investigate the changes of CYP4503A, a key enzyme of diazepam metabolism, and explore the effect of hemoperfusion treated plasma from patients with chronic severe hepatitis on the activity and expression of CYP4503A in C3A cells.


METHODS
Plasma was prepared and C3A cells were cultured. There were four groups in the experiment: normal fetal bovine plasma (NFBP) group, normal human plasma (NHP) group, hemoperfusion plasma (HPP) group, chronic severe hepatitis plasma (CSHP) group. The activity of erythromycin N-demethylase (ERD), namely the activity of CYP4503A, was measured by spectrophotometer and the expression of CYP4503A4 was detected by Western blot.


RESULTS
The activity of ERD in CSHP group and HPP group was lower than that in NFBP group (P<0.05) and NHP group (P<0.05) but the activity of ERD in HPP group was higher than that in CSHP group (P<0.05). The expression of CYP4503A4 increased in NFBP group (P<0.05) and NHP group (P<0.05) but the expression of CYP4503A4 in CSHP group decreased compared with HPP group(P<0.05).


CONCLUSIONS
The activity of ERD and the expression of CYP4503A decreased in CSHP group while the activity of ERD and the expression of CYP4503A in HPP group increased, which may cause the changes of the diazepam metabolic rates of C3A cells. This finding will provide some references for future researches on cell construction.
Yan
Li
L
Hepatitis Institute, Beijing You'an Hospital Affiliated to Capital Medical University, Beijing, China. yanli_come@sohu.com
Chen
Yu
Y
Zheng
Su-Jun
SJ
Zhao
Jun
J
Wu
Zhi-Ming
ZM
Duan
Zhong-Ping
ZP
D000328:Adult; D000818:Animals; D002460:Cell Line; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism*; D003975:Diazepam / Q000378:metabolism; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006464:Hemoperfusion; D006521:Hepatitis, Chronic / Q000201:enzymology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010949:Plasma
D051544:Cytochrome P-450 CYP3A; D003975:Diazepam