Single DNA molecules as probes for interrogating silica surfaces after various chemical treatments.
We examined the adsorption of single YOYO-1-labeled lambda-DNA molecules at glass surfaces after treatment with various chemical cleaning methods by using total internal reflection fluorescence microscopy (TIRFM). The characteristics of these surfaces were further assessed using contact angle (CA) measurements and atomic force microscopy (AFM). By recording the real-time dynamic motion of DNA molecules at the liquid/solid interface, subtle differences in adsorption affinities were revealed. The results indicate that the driving force for adsorption of DNA molecules on glass surfaces is mainly hydrophobic interaction. We also found that surface topography plays a role in the adsorption dynamics.
Liu
Xia
X
State Key Laboratory of Chemo/Biosensing and Chemometrics, BioMedical Engineering Center, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Wu
Zhan
Z
Nie
Huagui
H
Liu
Ziling
Z
He
Yan
Y
Yeung
E S
ES
D015342:DNA Probes / Q000737:chemistry* / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
D015342:DNA Probes; D012822:Silicon Dioxide