Clonality analysis of lymphoproliferative disorders in patients with Sjögren's syndrome.
The aim of this study was to clarify the nature of the clonal lymphocyte infiltration in Sjögren's syndrome (SS) patients associated with lymphoproliferative disorders. We examined B cell clonality in lymphoproliferative tissues from six primary SS patients associated with lymphoproliferative disorders or lymphoma by cloning and sequencing of the gene rearrangement of the immunoglobulin heavy chain complementarity determining region 3 (IgVH-CDR3). Three patients with sequential observation showed progressional clonal expansion with the presence of the same subclone in different tissues during the course of disease. Among them, one patient developed mucosa-associated lymphoid tissue (MALT) lymphoma in glandular parotid. The other three SS patients concomitant with malignant B cells lymphomas showed different clonal expansion of B cells between nodal sites and salivary glands. The cloanality analysis indicated that monoclonal B cell population could spread from one glandular site to another site during the course of SS, suggesting that the malignant clone may arise from the general abnormal microenvironment, not restricted to the glandular tissue, in some SS patients.
Dong
L
L
Department of Hematology and Immunology, Kanazawa Medical University, Ishikawa, Japan.
Masaki
Y
Y
Takegami
T
T
Jin
Z-X
ZX
Huang
C-R
CR
Fukushima
T
T
Sawaki
T
T
Kawanami
T
T
Saeki
T
T
Kitagawa
K
K
Sugai
S
S
Okazaki
T
T
Hirose
Y
Y
Umehara
H
H
D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000473:pathology*; D022801:Complementarity Determining Regions / Q000235:genetics; D018450:Disease Progression; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D016393:Lymphoma, B-Cell / Q000473:pathology; D018442:Lymphoma, B-Cell, Marginal Zone / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000473:pathology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012859:Sjogren's Syndrome / Q000473:pathology*
D022801:Complementarity Determining Regions; D007143:Immunoglobulin Heavy Chains