Nutrient consumption and production of isoflavones in bioreactor cultures of Pueraria lobata (Willd).
This paper reports the successful culture of Pueraria lobata (Willd.) suspension cells in a bioreactor. In vitro culture of this Chinese herb has potential as an alternative production method for industrial applications. Calli of P. lobata obtained from leaf explants were cultured in a 5.0 L bioreactor for two weeks. During this period, the pH of the medium declined from 5.8 to 4.5. By the end of the run about 70% of the sugars and reducing sugars and about 50% of nitrate was consumed. Almost 70% of inorganic phosphate and about 80% of the iron was exhausted. The bioreactor results indicated an isoflavone yield of 328.9 microg/ml, with an increase of about 1.77 fold. The yield of puerarin increased about 2.42 fold and reached 73.4 microg/ml in the bioreactor culture.
Chen
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G
College of Life Science, South China Normal University, Guangdong Key Lab of Biotechnology for Plant Development, Guangzhou 510 631, China.
Li
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L
D019149:Bioreactors; D050260:Carbohydrate Metabolism; D007501:Iron / Q000378:metabolism; D007529:Isoflavones / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D029908:Pueraria / Q000254:growth & development / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism
D007529:Isoflavones; D000644:Quaternary Ammonium Compounds; D010758:Phosphorus; D007501:Iron; D009584:Nitrogen