title: Detection of pim-1 mRNA in prostate cancer diagnosis.
abstract: BACKGROUND
  Pim-1 plays an important role in the apoptosis, proliferation, differentiation of cancer cells and progression of cancer. In this study we detected the expression of pim-1 mRNA in normal prostate, benign prostatic hyperplasia (BPH), and prostate cancer (PCa) and explored its diagnostic value for PCa.
  
  
  METHODS
  The prostate tissues were collected from 23 patients with PCa, 37 patients with BPH, and 3 healthy volunteers. Pim-1 mRNA expression levels in these samples were determined by the quantitative real-time PCR (QRT-PCR). The differences of expression were calculated based on a standard curve.
  
  
  RESULTS
  The ratio of pim-1 mRNA to beta-actin in the normal prostate, BPH, and PCa were 1.05 +/- 0.04, 2.57 +/- 0.74 and 4.45 +/-0.63, respectively. The differences among PCa, BPH and NT were significant (P < 0.05, respectively).
  
  
  CONCLUSIONS
  Detecting pim-1 mRNA expression by QRT-PCR provides a reliable metric for the diagnosis of PCa.
authors.affiliation: Department of Urology, First People's Hospital of Guangzhou, Guangzhou Medical College, Guangzhou 510180, China.
authors.forename: Hui-chan
authors.initials: HC
authors.lastname: He
authors.forename: Xue-cheng
authors.initials: XC
authors.lastname: Bi
authors.forename: Qi-shan
authors.initials: QS
authors.lastname: Dai
authors.forename: Shao-sheng
authors.initials: SS
authors.lastname: Wang
authors.forename: Hong-ai
authors.initials: HA
authors.lastname: Wei
authors.forename: Wei-de
authors.initials: WD
authors.lastname: Zhong
authors.forename: Wen-hua
authors.initials: WH
authors.lastname: Liu
authors.forename: Fu-neng
authors.initials: FN
authors.lastname: Jiang
authors.forename: Liang-shi
authors.initials: LS
authors.lastname: Liu
mesh_terms: D000368:Aged; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000378:metabolism; D011470:Prostatic Hyperplasia / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D051573:Proto-Oncogene Proteins c-pim-1 / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D012333:RNA, Messenger; C496634:PIM1 protein, human; D051573:Proto-Oncogene Proteins c-pim-1