title: An anti-adenoma antibody, Adnab-9, may reflect the risk for neoplastic progression in familial hamartomatous polyposis syndromes.
abstract: Patients with the hamartomatous polyposis Peutz-Jeghers and familial juvenile polyposis syndromes are predisposed to colorectal cancer but lack early genetic alterations found in adenomatous premalignant lesions. We studied hamartomatous polyps for the expression of an early preneoplastic colorectal neoplasia risk marker also found in familial adenomatous polyposis patients. Retrospective, genetic, and hospital archival tissue immunohistochemistry using Adnab-9, a premalignant marker often found in Paneth-like cells (PCs), was performed on sections of polyps from eight patients with Peutz-Jeghers syndrome, eight patients with familial juvenile polyposis, and 36 hyperplastic polyp control sections. Anti-alpha-defensin 5 (AD5), a universal PC marker, was also used to label a subgroup of sections. Hamartomatous polyposis patients also underwent specific genetic analysis. Eighty-nine percent of Peutz-Jeghers syndrome polyps labeled with Adnab-9 compared with 63% for AD5; 88% of familial juvenile polyposis sections also labeled with Adnab-9. Of the 36 hyperplastic polyp sections, only four (11%) labeled with Adnab-9 and one (3%) with AD5. Adnab-9 labeling of PCs in the epithelial elements of hamartomatous colonic lesions of hereditary hamartomatous syndrome patients reflects the predisposition to colorectal cancer, further justifying early intervention strategies.
authors.affiliation: John D. Dingell VAMC Department of Medicine, Wayne State University School of Medicine, Detroit, MI, USA. martin.tobi@va.gov
authors.forename: Martin
authors.initials: M
authors.lastname: Tobi
authors.forename: Michael
authors.initials: M
authors.lastname: Kam
authors.forename: Nadeem
authors.initials: N
authors.lastname: Ullah
authors.forename: Kashif
authors.initials: K
authors.lastname: Qureshi
authors.forename: Violeta
authors.initials: V
authors.lastname: Yordanova
authors.forename: James
authors.initials: J
authors.lastname: Hatfield
authors.forename: Suzanne E G
authors.initials: SE
authors.lastname: Fligiel
authors.forename: Paula
authors.initials: P
authors.lastname: Sochacki
authors.forename: Thomas
authors.initials: T
authors.lastname: McGarrity
authors.forename: Carolyn
authors.initials: C
authors.lastname: Cole
authors.forename: Michael
authors.initials: M
authors.lastname: Lawson
authors.forename: Russell
authors.initials: R
authors.lastname: Jacoby
mesh_terms: D000091162:AMP-Activated Protein Kinase Kinases; D011125:Adenomatous Polyposis Coli / Q000235:genetics / Q000473:pathology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D002675:Child, Preschool; D003106:Colon / Q000473:pathology*; D003111:Colonic Polyps / Q000235:genetics / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008297:Male; D008875:Middle Aged; D009385:Neoplastic Processes; D010580:Peutz-Jeghers Syndrome / Q000235:genetics / Q000473:pathology*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D023084:alpha-Defensins / Q000378:metabolism
chemical_list: C516879:Adnab-9; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; C500225:DEFA5 protein, human; D023084:alpha-Defensins; D017346:Protein Serine-Threonine Kinases; C109790:STK11 protein, human; D000091162:AMP-Activated Protein Kinase Kinases