title: Preoperative sterilization and disinfection of drill guide templates.
abstract: The aim of the in vitro study was to evaluate the decontamination potential of common antiseptic solutions for heat-sensitive implantological drill guide templates. One hundred implantologists were evaluated on the basis of a questionnaire for their measures of disinfection. On the basis of these results, 80% alcohol, Octenidine 0.1%, and Chlorhexidine 0.12% were tested in an in vitro model for their decontamination efficacy for heat-sensitive plastic material infected with Pseudomonas aeruginosa, Acinetobacter baumannii, Enterococcus faecalis, Enterococcus faecium, Staphylococcus aureus, Enterobacter cloacae, Escherichia coli, and Candida albicans. The microorganisms were selected on the basis of results of environmental testing of dental laboratories. The results of the questionnaire revealed that Chlorhexidine was used by 30%, 80% alcohol by 23%, and Octenidine by 7% of the dentists. Using the in vitro model, with the exception of S. aureus, Chlorhexidine was not able to completely eliminate the microorganisms after 15 min of application. In contrast, the treatment with Octenidine revealed no further growth of the tested microorganisms after that time. The 80% alcohol was more efficient. No growth of microorganisms could be detected in any of the tests after 5 min of incubation. On the basis of our results and due to the fact that suitable installations for sterilization were hardly used by the dental practitioners, the disinfection of templates should be preferentially performed with 80% alcohol or Octenidine using an incubation time of 15 min with ultrasonication.
authors.affiliation: Department of Oral Surgery, Georg-August University, Göttingen, Germany. se.ki@med.uni-goettingen.de
authors.forename: Sabine
authors.initials: S
authors.lastname: Sennhenn-Kirchner
authors.forename: Sascha
authors.initials: S
authors.lastname: Weustermann
authors.forename: Hamparsum
authors.initials: H
authors.lastname: Mergeryan
authors.forename: Hans Georg
authors.initials: HG
authors.lastname: Jacobs
authors.forename: Margarete
authors.initials: M
authors.lastname: Borg-von Zepelin
authors.forename: Bernhard
authors.initials: B
authors.lastname: Kirchner
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use; D001419:Bacteria / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects; D002710:Chlorhexidine / Q000627:therapeutic use; D019551:Dental Disinfectants / Q000627:therapeutic use*; D003758:Dental Implantation, Endosseous / Q000295:instrumentation* / Q000382:microbiology; D004203:Disinfection / Q000379:methods*; D016972:Enterobacter cloacae / Q000187:drug effects; D013293:Enterococcus faecalis / Q000187:drug effects; D016984:Enterococcus faecium / Q000187:drug effects; D004867:Equipment Design; D004926:Escherichia coli / Q000187:drug effects; D000431:Ethanol / Q000627:therapeutic use; D006801:Humans; D007097:Imines; D010969:Plastics / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D011725:Pyridines / Q000627:therapeutic use; D013211:Staphylococcus aureus / Q000187:drug effects; D013242:Sterilization / Q000379:methods*; D013997:Time Factors
chemical_list: D000891:Anti-Infective Agents, Local; D019551:Dental Disinfectants; D007097:Imines; D010969:Plastics; D011725:Pyridines; D000431:Ethanol; D019904:Polymethyl Methacrylate; C034213:octenidine; D002710:Chlorhexidine