title: Analysis of peptide mimotopes of Burkholderia pseudomallei exopolysaccharide.
abstract: Previously two capsule-specific monoclonal antibodies (4VA5 and 3VIE5) were identified as protective against Burkholderia pseudomallei in passive transfer experiments. Panning these antibodies against evolutionary phage libraries identified reactive peptides capable of inhibiting its parent monoclonal from binding to B. pseudomallei. Mice immunized with peptide conjugated to thyroglobulin developed serum antibodies capable of recognizing the immunizing peptide of which a subset recognized exopolysaccharide in the context of whole B. pseudomallei cells. These serum antibodies recognized protease treated B. pseudomallei but not B. thailandensis suggesting that these peptides are mimotopes of the B. pseudomallei capsular exopolysaccharide. In a murine model of acute melioidosis, immunization with the mimotope of the 4VA5 binding site extended the mean time to death to 8.00 days over the 2.18 days afforded by immunization with thyroglobulin alone. This mimotope may be of use in developing an antibody response against B. pseudomallei exopolysaccharide.
authors.affiliation: Department of Microbiology, The Ohio State University, 484 W 12th Ave Rm 376, Columbus, OH 43210, United States. Bart.Legutki@asu.edu
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Legutki
authors.forename: Michelle
authors.initials: M
authors.lastname: Nelson
authors.forename: Richard
authors.initials: R
authors.lastname: Titball
authors.forename: Darrell R
authors.initials: DR
authors.lastname: Galloway
authors.forename: Alfred
authors.initials: A
authors.lastname: Mateczun
authors.forename: Leslie W
authors.initials: LW
authors.lastname: Baillie
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D016957:Burkholderia pseudomallei / Q000737:chemistry* / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D008554:Melioidosis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D010455:Peptides / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D000939:Epitopes; D010455:Peptides; D011135:Polysaccharides, Bacterial