Autophagy and apoptosis in Chinese hamster ovary cell culture.
Upon nutrient deprivation during Chinese hamster ovary (CHO) cell culture for foreign protein production, cells are subjected to two types of programmed cell death (PCD), apoptosis and autophagy. However, only apoptosis has drawn attention in the field of CHO cell culture. Numerous studies on engineering genes or supplementing essential nutrients or chemical additives to culture media to overcome cell death induced by various stimuli have been limited to apoptosis. Recently, autophagic morphologies were demonstrated by the processing of LC3 into the 16 kDa LC3-II form, and the accumulation of multiple autophagosomes in CHO cell culture. Therefore, it seems worthwhile to revisit the issue of cell death in CHO cell culture with the concept of autophagy in mind, in order to achieve a maximum production of foreign proteins by protecting cells from both types of PCD.
Hwang
Sun Ok
SO
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.
Lee
Gyun Min
GM
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001343:Autophagy / Q000502:physiology*; D016466:CHO Cells / Q000166:cytology / Q000502:physiology*; D018929:Cell Culture Techniques; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media / Q000737:chemistry; D008869:Microtubule-Associated Proteins / Q000378:metabolism
D003470:Culture Media; D008869:Microtubule-Associated Proteins