title: [Frequency of detection of Ureaplasma urealyticum and Mycoplasma hominis in cervical canal and the Douglas pouch of infertile and fertile women].
abstract: The group of organisms commonly referred to as genital mycoplasmas comprise species most often found in genitourinary tract of sexually active adults as common commensal inhabitants, or pathogens which can possibly cause many different pathologies like: non-gonococcal urethritis, bacterial vaginosis, cervicitis, endometritis or pelvic inflammatory disease. The problem of their morbidity and the possible influence they have on human fertility is still not clear. The aim of this study was to find out whether two investigated species- Ureaplasma urealyticum and Mycoplasma hominis can be detect more often in a group of infertile women. 74 women participated in the study and were assigned to one of 2 groups of patients: infertile women and fertile women without any sign of genital tract infection. Swabs from the cervical canal of the uterus and the fluid from the Douglas pouch were taken during the gynecological examination and laparoscopic procedure. Two diagnostic methods were used: biochemical method- commercial diagnostic kit- Mycoplasma IST 2 and PCR method. The results showed that Ureaplasma urealyticum and Mycoplasma hominis were detected among both fertile and infertile women with nearly the same frequency, much more often in cervical canal than in the Douglas pouch. Ureaplasma urealyticum was more common pathogen than Mycoplasma hominis in both groups and locations. The achieved results point out that the role of genital mycoplasmas in human infertility is still unclear and require further investigations.
authors.affiliation: Klinika Ginekologii II Katedry Ginekologii i Połoznictwa AM we Wrocławiu.
authors.forename: Joanna
authors.initials: J
authors.lastname: Grześko
authors.forename: Marek
authors.initials: M
authors.lastname: Elias
authors.forename: Beata
authors.initials: B
authors.lastname: Maczyńska
authors.forename: Urszula
authors.initials: U
authors.lastname: Kasprzykowska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Tłaczała
authors.forename: Marian
authors.initials: M
authors.lastname: Goluda
mesh_terms: D000328:Adult; D002584:Cervix Uteri / Q000382:microbiology*; D015331:Cohort Studies; D004312:Douglas' Pouch / Q000382:microbiology*; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000382:microbiology*; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000382:microbiology; D019535:Mycoplasma hominis / Q000302:isolation & purification*; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D012307:Risk Factors; D016869:Ureaplasma Infections / Q000175:diagnosis / Q000382:microbiology; D016990:Ureaplasma urealyticum / Q000302:isolation & purification*; D002575:Uterine Cervicitis / Q000382:microbiology