title: Potential of plant polyphenol oxidases in the decolorization and removal of textile and non-textile dyes.
abstract: In this study an effort has been made to use plant polyphenol oxidases; potato (Solanum tuberosum) and brinjal (Solanum melongena), for the treatment of various important dyes used in textile and other industries. The ammonium sulphate fractionated enzyme preparations were used to treat a number of dyes under various experimental conditions. Majority of the treated dyes were maximally decolorized at pH 3.0. Some of the dyes were quickly decolorized whereas others were marginally decolorized. The initial first hour was sufficient for the maximum decolorization of dyes. The rate of decolorization was quite slow on long treatment of dyes. Enhancement in the dye decolorization was noticed on increasing the concentration of enzymes. The complex mixtures of dyes were treated with both preparations of polyphenol oxidases in the buffers of varying pH values. Potato polyphenol oxidase was significantly more effective in decolorizing the dyes to higher extent as compared to the enzyme obtained from brinjal polyphenol oxidase. Decolorization of dyes and their mixtures, followed by the formation of an insoluble precipitate, which could be easily removed simply by centrifugation.
authors.affiliation: Department of Biochemistry, Faculty of Life Science, Aligarh Muslim University, Aligarh-202 002, India.
authors.forename: Amjad Ali
authors.initials: AA
authors.lastname: Khan
authors.forename: Qayyum
authors.initials: Q
authors.lastname: Husain
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D002384:Catalysis; D004156:Catechol Oxidase / Q000737:chemistry*; D003116:Color; D004396:Coloring Agents / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D032323:Solanum melongena / Q000737:chemistry / Q000201:enzymology*; D011198:Solanum tuberosum / Q000737:chemistry / Q000201:enzymology*; D013783:Textile Industry; D014865:Waste Disposal, Fluid / Q000379:methods; D014874:Water Pollutants, Chemical / Q000737:chemistry*; D018508:Water Purification / Q000379:methods
chemical_list: D004396:Coloring Agents; D007220:Industrial Waste; D014874:Water Pollutants, Chemical; D004156:Catechol Oxidase; D000645:Ammonium Sulfate