[Immunologic tolerance of anti-Abeta antibody in the sera of AD patients].
OBJECTIVE
To characterize anti-amyloid-beta antibodies in the sera of Alzheimer's disease(AD) patients.


METHODS
A special tissue amyloid plaque immunoreactivity (TAPIR) examinations of brains of Tg2576 mice was carried out. Abeta(42)-GST fusion protein was detected by Western blot. and then the survival rate of PC12 cells incubated with the sera and Abeta(42) was measured by MTT assay.


RESULTS
The sera in AD patients could not recognize the senile plaque in the brains of Tg2576 mice and could not cross-react with Abeta(42)-GST fusion protein. Furthermore, PC12 cells incubated with the sera from AD patients presented the decreased A value compared with the healthy elderly(P<0.01).


CONCLUSIONS
The anti-Abeta antibodies in the sera of AD patients may have immunologic tolerance to amyloid peptide.
Yang
Zhi-Yong
ZY
Department of Anatomy, School of Medicine, Southern Yangtze University, Wuxi, China. fy.zy.yang@163.com
Wang
Hua-Qiao
HQ
Xu
Jie
J
Xie
Yao
Y
Yuan
Qun-Fang
QF
Yao
Zhi-Bin
ZB
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000097:blood* / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D016229:Amyloid beta-Peptides / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000097:blood* / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D016022:Case-Control Studies; D003429:Cross Reactions; D006801:Humans; D007108:Immune Tolerance; D007150:Immunohistochemistry; D051379:Mice; D008875:Middle Aged; D016716:PC12 Cells; D058225:Plaque, Amyloid / Q000276:immunology; D051381:Rats
D016229:Amyloid beta-Peptides; D000906:Antibodies