title: Identifying common metalloprotease inhibitors by protein fold types using Fourier transform mass spectrometry.
abstract: Fourteen natural products, known to inhibit other proteins of the Zincin-like fold class, were screened for inhibition of the Zincin-like fold metalloprotease thermolysin using mass spectrometry. Fourier Transform Mass Spectrometry was successful in identifying actinonin, a known inhibitor of astacin and stromelysin, to be an inhibitor of thermolysin. Molecular modelling studies have shown that specificity within the Zincin-like fold is determined by Protein Fold Topology.
authors.affiliation: Eskitis Institute, Griffith University, Brisbane, Qld 4111, Australia.
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Mitchell
authors.forename: Desley
authors.initials: D
authors.lastname: Pitcher
authors.forename: Bernadette M
authors.initials: BM
authors.lastname: McArdle
authors.forename: Terese
authors.initials: T
authors.lastname: Alnefelt
authors.forename: Sandra
authors.initials: S
authors.lastname: Duffy
authors.forename: Vicky
authors.initials: V
authors.lastname: Avery
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Quinn
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods; D006877:Hydroxamic Acids / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D045726:Metalloproteases / Q000037:antagonists & inhibitors*; D011480:Protease Inhibitors / Q000032:analysis / Q000737:chemistry*; D017510:Protein Folding; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013820:Thermolysin / Q000737:chemistry / Q000378:metabolism
chemical_list: D006877:Hydroxamic Acids; D011480:Protease Inhibitors; D011506:Proteins; D045726:Metalloproteases; D013820:Thermolysin; C010531:actinonin