Identification of differentially expressed genes in distinct skeletal muscles in cattle using cDNA microarray.
The 788-gene microarray was manufactured using selected elements from three different cDNA libraries in order to identify molecular processes that determine phenotypic characteristics between loin (M. longissimus thoracis) and round (M. semimembranosus) muscles. Microarray analyses identified 24 differentially expressed genes between the two muscles investigated. Five of the genes were verified by quantitative RT-PCR and three of them were mapped on bovine chromosomes using 5,000 rad bovine radiation hybrid (RH) panel. The map locations indicated that they were mapped in the same chromosomal regions where IMF and growth QTLs were located, suggesting that they are most possible positional candidate genes for the traits.
Yu
S L
SL
Division of Animal Science and Resources, Chungnam National University, Daejeon, Korea.
Chung
H J
HJ
Sang
B C
BC
Park
C S
CS
Lee
J H
JH
Yoon
D H
DH
Lee
S H
SH
Choi
K D
KD
D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods / Q000662:veterinary; D008460:Meat; D018485:Muscle Fibers, Skeletal / Q000502:physiology*; D018482:Muscle, Skeletal / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary; D021101:Radiation Hybrid Mapping / Q000662:veterinary