title: Carbohydrate analysis by desorption electrospray ionization fourier transform ion cyclotron resonance mass spectrometry.
abstract: We report the use of desorption electrospray ionization hybrid Fourier transform ion cyclotron resonance mass spectrometry (DESI-FT-ICR-MS) for the analysis of carbohydrates. Spectra of neat carbohydrates are presented along with their mass measurement accuracies and limits of detection. Furthermore, a comparison is made between the analyses of O-linked glycans from mucin by DESI-FT-ICR-MS and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry. Finally, glycans from mucin are identified by using the high mass measurement accuracy and tandem MS capabilities afforded by the hybrid FT-ICR-MS platform.
authors.affiliation: W.M Keck FT-ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Bereman
authors.forename: Taufika Islam
authors.initials: TI
authors.lastname: Williams
authors.forename: David C
authors.initials: DC
authors.lastname: Muddiman
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D017357:Cyclotrons; D005583:Fourier Analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D002241:Carbohydrates