Phosphoproteomic analysis of AML14.3D10 cell line as a model system of eosinophilia.
Eosinophils act as effectors in the inflammatory reactions of allergic diseases including atopic dermatitis. Atopic dermatitis patients and others with allergic disorders suffer from eosinophilia, an accumulation of eosinophils due to increased survival or decreased apoptosis of eosinophils. In this study, a differential phosphoproteome analysis of AML14.3D10 eosinophil cell line after treatment with IL-5 or dexamethasone was conducted in an effort to identify the phosphoproteins involved in the proliferation or apoptosis of eosinophils. Proteins were separated by 2-DE and alterations in phosphoproteins were then detected by Pro-Q Diamond staining. The significant quantitative changes were shown in nineteen phosphoproteins including retinoblastoma binding protein 7, MTHSP75, and lymphocyte cytosolic protein 1. In addition, seven phosphoproteins including galactokinase I, and proapolipoprotein, were appeared after treatment with IL-5 or dexamethasone. Especially, the phospho-APOE protein was down-regulated in IL-5 treated AML14.3D10, while the more heavily phosphorylated APOE form was induced after dexamethasone treatment. These phosphoproteome data for the AML14.3D10 cell line may provide clues to understand the mechanism of eosinophilia as well as allergic disorders including atopic dermatitis.
Ryu
Su In
SI
Translational Research Center, KRIBB, Daejeon, 305-806, South Korea.
Kim
Won Kon
WK
Cho
Hyun Ju
HJ
Lee
Phil Young
PY
Jung
Hyeyun
H
Yoon
Tae-Sung
TS
Moon
Jeong Hee
JH
Kang
Sunghyun
S
Poo
Haryoung
H
Bae
Kwang-Hee
KH
Lee
Sang Chul
SC
D002460:Cell Line; D016208:Databases, Factual; D003907:Dexamethasone / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D004802:Eosinophilia / Q000378:metabolism*; D006801:Humans; D015848:Interleukin-5 / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010750:Phosphoproteins / Q000032:analysis* / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
D015848:Interleukin-5; D010750:Phosphoproteins; D020543:Proteome; D003907:Dexamethasone