Vestigial-like-2b (VITO-1b) and Tead-3a (Tef-5a) expression in zebrafish skeletal muscle, brain and notochord.
The vestigial gene has been shown to control skeletal muscle formation in Drosophila and the related Vestigial-like 2 (Vgl-2) protein plays a similar role in mice. Vgl-family proteins are thought to regulate tissue-specific gene expression by binding to members of the broadly expressed Scalloped/Tef/TEAD transcription factor family. Zebrafish have at least four Vgl genes, including two Vgl-2s, and at least three TEAD genes, including two Tead3s. We describe the cloning and expression of one member from each family in the zebrafish. A novel gene, vgl-2b, with closest homology to mouse and human vgl-2, is expressed transiently in nascent notochord and in muscle fibres as they undergo terminal differentiation during somitogenesis. Muscle cells also express a TEAD-3 homologue, a possible partner of Vgl-2b, during myoblast differentiation and early fibre assembly. Tead-3a is also expressed in rhombomeres, eye and epiphysis regions.
Mann
Christopher J
CJ
MRC Centre for Developmental Neurobiology and Randall Division for Cell and Molecular Biophysics, King's College London, London SE1 1UL, UK.
Osborn
Daniel P S
DP
Hughes
Simon M
SM
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002490:Central Nervous System / Q000737:chemistry / Q000196:embryology; D003001:Cloning, Molecular; D015870:Gene Expression; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000737:chemistry / Q000196:embryology / Q000378:metabolism; D009672:Notochord / Q000737:chemistry / Q000196:embryology / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D000091262:TEA Domain Transcription Factors; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins / Q000737:chemistry / Q000235:genetics*
D012333:RNA, Messenger; D000091262:TEA Domain Transcription Factors; D014157:Transcription Factors; D029961:Zebrafish Proteins; C526785:tead3a protein, zebrafish; C526784:vgll2b protein, zebrafish