[The construction of ADAM28 eukaryotic expression plasmid and expression after transfected into HDFC].
OBJECTIVE
To construct a disintegrin and metalloproteinase (ADAM28) eukaryotic expression plasmid and detect the expression of ADAM28 gene in human dental follicle cells (HDFC) after eukaryotic plasmid was transfected into HDFC.


METHODS
Gene rebuilt technique was used to construct ADAM28 eukaryotic expression plasmid, cell culture and gene transfection into HDFC mediated by Lipofectamine2000, immunofluorescence, RT-PCR and Western blot were used to detect the expression of ADAM28 in HDFC.


RESULTS
ADAM28 eukaryotic plasmid was constructed, identified successfully and transiently transfected into HDFC for 72 hours. The detection verified that HDFC of eukaryotic plasmid group all expressed ADAM28 protein, and no expression was found in two control groups.


CONCLUSIONS
ADAM28 could be correctly translated and expressed in HDFC, which might be used for further study of biological functions of ADAM28 in odontogenic cells.
Zhao
Zheng
Z
Department of Stomatology, The 401 Hospital of PLA, Qingdao 266071, China. zhaozheng170@sohu.com
Huang
Zheng-nan
ZN
Xu
Jun-ming
JM
D051722:ADAM Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D003795:Dental Sac / Q000166:cytology*; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids / Q000235:genetics; D014162:Transfection
D051722:ADAM Proteins; C410224:ADAM28 protein, human