Simultaneous analysis of multiple serum proteins adhering to the surface of medical grade polydimethylsiloxane elastomers.
Although polydimethylsiloxane (PDMS, silicone) elastomers are presumed to be chemically inert and of negligible toxicity, they induce a prompt acute inflammatory response with subsequent fibrotic reactions. Since local inflammatory and fibrotic side effects are associated with the proteinaceous film on the surface of silicone implants, the process of protein adherence to silicone is of practical medical relevance, and interesting from theoretical, clinical and biotechnological perspectives. It is hypothesized that the systemic side effects resembling rheumatoid and other connective tissue diseases may be triggered by local immunological changes, but this functional relationship has yet to be defined. Because the proteinaceous film on the surface of silicone has been identified as a key player in the activation of host defense mechanisms, we propose a test system based on a proteomics screen to simultaneously identify proteins adsorbed from serum to the surface of silicone. Herein, we describe protein adsorption kinetics on the surface of silicone implants, correlate the adhesion properties of serum proteins with the occurrence of adverse reactions to silicone, and successfully discriminate their signature on the silicone surface in a blinded study of patients suffering from fibrotic reactions (as determined by Baker scale) to silicone implants.
Backovic
Aleksandar
A
Division for Experimental Pathophysiology and Immunology, Biocenter, Innsbruck Medical University, Fritz-Pregl Str 3/4, A-6020, Innsbruck, Austria. Aleksandar.Backovic@i-med.ac.at
Wolfram
Dolores
D
Del-Frari
Barbara
B
Piza
Hildegunde
H
Huber
Lukas A
LA
Wick
Georg
G
D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry; D001798:Blood Proteins / Q000032:analysis* / Q000378:metabolism; D001940:Breast / Q000473:pathology; D018427:Breast Implants / Q000009:adverse effects*; D004129:Dimethylpolysiloxanes / Q000009:adverse effects / Q000737:chemistry; D005260:Female; D005355:Fibrosis; D006801:Humans; D007163:Immunosorbent Techniques; D008875:Middle Aged; D012826:Silicone Elastomers / Q000009:adverse effects* / Q000737:chemistry*; D012828:Silicones / Q000009:adverse effects / Q000737:chemistry
D001672:Biocompatible Materials; D001798:Blood Proteins; D004129:Dimethylpolysiloxanes; D012826:Silicone Elastomers; D012828:Silicones; C013830:baysilon