Augmented humoral and cellular immune responses induced by canine adenovirus type 1 DNA vaccine in BALB/c mice.
Infectious canine hepatitis (ICH) is caused by canine adenovirus type 1 (CAV-1), which severely harms infected animals. Vaccination provides an effective approach to preventing canine infectious diseases. With the objective of exploring a new vaccination strategy that may prevent or cure ICH, we constructed a DNA vaccine, pVAX1-CpG-Loop, and evaluated its immune efficacy. We found that vaccination of BALB/c mice with the DNA vaccine alone, or priming with DNA vaccine and boosting with the Loop protein, resulted in the following: (1) High-level specific antibody (IgG) against CAV-1 was induced; (2) T cell activation was elicited; and (3) neutralizing antibodies were detectable in immunized mice. Collectively, these data indicate that the availability of a DNA vaccine could prevent hepatitis contagiosa canis.
Wang
Jun-Xia
JX
Department of Molecular Biology, Hebei Medical University, Shijiazhuang, People's Republic of China.
Zheng
Long
L
Song
Shu-Xia
SX
Zhang
Xia
X
Li
Li-Min
LM
Wang
Fang
F
Liu
Ying Fu
YF
Sun
Shu-Han
SH
D006516:Adenoviruses, Canine / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004285:Dogs; D005260:Female; D006522:Hepatitis, Infectious Canine / Q000276:immunology*; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000097:blood; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D013601:T-Lymphocytes / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D022223:Vaccines, Subunit