cagA gene and protein status among Iranian Helicobacter pylori strains.
The wide geographic genetic diversity of Helicobacter pylori (Hp) and, in particular, the varying prevalence of cagA in different countries has been documented repeatedly. This study was designed to determine the frequency of cagA in Iranian Hp strains by means of genotyping and assessment of host antibodies. Helicobacter pylori strains from 235 patients, including 174 non-ulcer dyspepsia, 25 peptic ulcer and 36 gastric cancer patients, were studied. The frequencies of the 5', middle and 3' terminal regions of the cagA gene were 90.6, 57.6, 89%, respectively, with no correlation to the clinical outcomes. Antibodies against the CagA protein were present in 90.7% of patients. Multiple biopsy sampling in 97 cases revealed multiple infection in 16.5% of the patients. Sequencing of the seven variants of the 3' end of the cagA gene revealed no clustering and the distribution of the Iranian strains among those of other countries. Our results from the genotyping and serology analyses confirm that the majority of Iranian Hp strains are cagA-positive.
Talebkhan
Yeganeh
Y
Biotechnology Research Center, Pasteur Institute of Iran, Tehran 13164, Iran.
Mohammadi
Marjan
M
Mohagheghi
Mohammad Ali
MA
Vaziri
Hamid Reza
HR
Eshagh Hosseini
Mahmoud
M
Mohajerani
Nazanin
N
Oghalaei
Akbar
A
Esmaeili
Maryam
M
Zamaninia
Leili
L
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D015331:Cohort Studies; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D010437:Peptic Ulcer / Q000097:blood / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D011237:Predictive Value of Tests; D016036:Seroepidemiologic Studies; D013274:Stomach Neoplasms / Q000097:blood / Q000382:microbiology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori