title: Alterations in immune function and CYP450 activity in adult male deer mice (Peromyscus maniculatus) following exposure to benzo[a]pyrene, pyrene, or chrysene.
abstract: Polycyclic aromatic hydrocarbons (PAHs) are among the most common classes of chemical contaminants found at hazardous waste sites. Deer mice (Peromyscus maniculatus) exhibit a wide geographic distribution throughout North America and have been suggested as a terrestrial biomonitoring species to facilitate comparisons between superfund sites. Chemicals tested were benzo[a]pyrene (BaP; CAS number 50-32-8), pyrene (Pyr; CAS number 129-00-0), and chrysene (Chr; CAS number 218-01-9). Adult male deer mice were exposed via intraperitoneal (i.p.) injection every other day for 11 d to the PAHs (0.3, 1, 3, 10, or 30 mg/kg) or a corn oil carrier control. Both BaP and Chr suppressed the plaque-forming cell (PFC) response at all treatment levels. Pyr exposure (1-30 mg/kg) also resulted in suppression of this response. Macrophage pinocytosis was suppressed only by Chr (3, 10, and 30 mg/kg). Concanavalin A-induced proliferation was stimulated by BaP at all dose levels, by Pyr at 1-30 mg/kg, and by Chr at 30 mg/kg. Chr did not affect pokeweed mitogen (PWM)-induced proliferation; however, BaP (1-30 mg/kg) and Pyr (0.3-30 mg/kg) produced stimulation of this response as compared to respective controls. BaP and Chr stimulated cytochrome P-450 1A1 (CYP1A1) activity (3, 10, or 30 mg/kg) as measured by ethoxyresorufin O-deethylase (EROD) activity, but Pyr did not. These results indicate that immune function endpoints appear to be more sensitive to these PAHs than measured hepatic CYP450 activity.
authors.affiliation: Department of Pediatrics and the Marine Biomedicine and Environmental Science Center, Medical University of South Carolina, Charleston, South Carolina 29412, USA. pedenada@musc.edu
authors.forename: M M
authors.initials: MM
authors.lastname: Peden-Adams
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Knutson
authors.forename: J
authors.initials: J
authors.lastname: Dancik
authors.forename: K
authors.initials: K
authors.lastname: Bryant
authors.forename: A B
authors.initials: AB
authors.lastname: Bodine
authors.forename: R L
authors.initials: RL
authors.lastname: Dickerson
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D015415:Biomarkers; D002273:Carcinogens / Q000633:toxicity*; D002911:Chrysenes / Q000633:toxicity*; D019363:Cytochrome P-450 CYP1A1 / Q000187:drug effects / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring; D004789:Enzyme Activation / Q000187:drug effects; D007107:Immune System / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D010542:Peromyscus; D010873:Pinocytosis / Q000187:drug effects* / Q000276:immunology; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D011721:Pyrenes / Q000633:toxicity*; D013154:Spleen / Q000187:drug effects / Q000276:immunology
chemical_list: D015415:Biomarkers; D002273:Carcinogens; D002911:Chrysenes; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; C031180:chrysene; D001564:Benzo(a)pyrene; D003577:Cytochrome P-450 Enzyme System; C030984:pyrene; D019363:Cytochrome P-450 CYP1A1