title: Fluorescent DNA probes: study of mechanisms of changes in spectral properties and features of practical application.
abstract: Spectral data and nucleic acid complex formation properties for more than 30 both newly synthesized and widely used fluorescent nucleotide-specific compounds of various classes have been analyzed. These include phenylbenzene, bisbenzimidazole, psoralen, angelicin, tetrahydrocarbazole, oxophenoxazine, and others. The main rules of a generalized model adequately explaining changes in fluorescent properties of synthetic, low molecular weight nucleotide-specific dyes depending on their chemical structure, mode of interaction with substrate, properties of assay medium, etc. are proposed. Fluorescent nucleotide-specific dyes have been originally used in newly developed methods for: express evaluation of "generalized microbial dissemination" of liquid media; evaluation of possible genotoxic effects of various foodstuffs, pharmaceutical drugs, hazardous environmental factors (including their combined effects on living organisms), etc.
authors.affiliation: Mendeleyev Russian Research Institute for Metrology, St. Petersburg, 190005, Russia. vs1969r@mail.ru
authors.forename: V S
authors.initials: VS
authors.lastname: Sibirtsev
mesh_terms: D000075202:Contraindications; D004249:DNA Damage; D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry*; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry; D004353:Drug Evaluation, Preclinical; D005456:Fluorescent Dyes / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D005456:Fluorescent Dyes