Analysis on DNA methylation of various tissues in chicken.
DNA methylation is known to be associated with the transcriptional silencing in eukaryotes. In this study, the genome-wide DNA methylation patterns of CCGG sites were detected with fluorescent-labeled methylation sensitive amplified polymorphism (F-MSAP) in three chicken strains (White Leghorn, White Plymouth Rock, and their F(1) hybrids). The average incidence of DNA methylation was approximately 29.4% in muscle, 27.2% in liver, 27.2% in heart, and 26.1% in kidney, respectively. Also, the significant differences of the DNA methylation levels were found among the different tissues (P < 0.05) or strains (P < 0.05), implying that such differences may be related to gene expression during tissue differentiation, growth, and development though the relation between methylation and gene expression is currently hard to assess.
Xu
Qing
Q
Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Zhang
Yuan
Y
Sun
Dongxiao
D
Wang
Yachun
Y
Yu
Ying
Y
D000818:Animals; D015139:Blotting, Southern; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D018899:CpG Islands; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic
D004247:DNA