Comparative proteome profiling and functional analysis of chronic myelogenous leukemia cell lines.
The aim of the present study was the molecular profiling of different Ph+ chronic myelogenous leukemia (CML) cell lines (LAMA84, K562, and KCL22) by a proteomic approach. By employing two-dimensional gel electrophoresis combined with mass spectrometry analysis, we have identified 191 protein spots corresponding to 142 different proteins. Among these, 63% were cancer-related proteins and 74% were described for the first time in leukemia cells. Multivariate analysis highlighted significant differences in the global proteomic profile of the three CML cell lines. In particular, the detailed analysis of 35 differentially expressed proteins revealed that LAMA84 cells preferentially expressed proteins associated with an invasive behavior, while K562 and KCL22 cells preferentially expressed proteins involved in drug resistance. These data demonstrate that these CML cell lines, although representing the same pathological phenotype, show characteristics in their protein expression profile that suggest different phenotypic leukemia subclasses. These data contribute a new potential characterization of the CML phenotype and may help to understand interpatient variability in the progression of disease and in the efficacy of a treatment.
Fontana
Simona
S
Sezione di Biologia e Genetica, Dipartimento di Biopatologia e Metodologie Biomediche, Università di Palermo, Palermo, Italy.
Alessandro
Riccardo
R
Barranca
Marilisa
M
Giordano
Margherita
M
Corrado
Chiara
C
Zanella-Cleon
Isabelle
I
Becchi
Michel
M
Kohn
Elise C
EC
De Leo
Giacomo
G
D000165:Acridine Orange / Q000494:pharmacology; D045744:Cell Line, Tumor; D002465:Cell Movement; D018450:Disease Progression; D019008:Drug Resistance, Neoplasm; D015180:Electrophoresis, Gel, Two-Dimensional; D004996:Ethidium / Q000494:pharmacology; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000378:metabolism*; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D010641:Phenotype; D040901:Proteomics / Q000379:methods*
D004996:Ethidium; D000165:Acridine Orange