Spectroscopic probing of ortho-nitrophenol localization in phospholipid bilayers.
In the present study we estimated the localization of ortho-nitrophenol (ONP) within model membranes through its efficiency to quench and to modify the anisotropy of DPH and TMA-DPH fluorescence. These fluorescent probes are known to sense the hydrocarbon core and the polar head group region of membranes, respectively. TMA-DPH fluorescence in MLVs was more efficiently quenched than DPH (K(q,TMA-DPH)=2.36 and K(q,DPH)=1.07 mM ns(-1)). Moreover, these results demonstrated the interfacial localization of ONP and may contribute to understand membrane-mediated mechanisms of ONP-induced toxicity and the behavior of ONP as a product of several enzymatic reactions occurring in the presence of lipid-water interfaces.
Sánchez
Julieta M
JM
Biofisica-Química, Depto. de Química, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Av. Velez Sarsfield 1611, X 5016GCA Córdoba, Argentina.
Turina
Anahí del V
AV
Perillo
María A
MA
D004161:Diphenylhexatriene / Q000031:analogs & derivatives / Q000737:chemistry; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D009596:Nitrophenols / Q000032:analysis* / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D009596:Nitrophenols; D010743:Phospholipids; D004161:Diphenylhexatriene; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene; C045573:2-nitrophenol