Novel decapeptides that bind avidly and deliver radioisotope to colon cancer cells.
BACKGROUND
The rapidly growing field of targeted tumor therapy often utilizes an antibody, sometimes tagged with a tumor-ablating material such as radioisotope, directed against a specific molecule.


RESULTS
This report describes the discovery of nine novel decapeptides which can be radioactively labeled, bind to, and deliver (32)P to colon cancer cells. The decapeptides vary from one another by one to three amino acids and demonstrate vastly different binding abilities. The most avidly binding decapeptide can permanently deliver very high levels of radioisotope to the adenocarcinoma cancer cell lines at an efficiency 35 to 150 times greater than to a variety of other cell types, including cell lines derived from other types of cancer or from normal tissue.


CONCLUSIONS
This experimental approach represents a new example of a strategy, termed peptide binding therapy, for the potential treatment of colorectal and other adenocarcinomas.
Abraham
John M
JM
Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America. jabrah14@jhmi.edu
Sato
Fumiaki
F
Cheng
Yulan
Y
Paun
Bogdan
B
Kan
Takatsugu
T
Olaru
Alexandru
A
Jin
Zhe
Z
Yang
Jian
J
Agarwal
Rachana
R
David
Stefan
S
Hamilton
James P
JP
Ito
Tetsuo
T
Mori
Yuriko
Y
Meltzer
Stephen J
SJ
D000230:Adenocarcinoma / Q000188:drug therapy* / Q000532:radiotherapy*; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000532:radiotherapy*; D016503:Drug Delivery Systems; D015195:Drug Design; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D008495:Medical Oncology / Q000379:methods; D010455:Peptides / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000008:administration & dosage; D010761:Phosphorus Radioisotopes / Q000008:administration & dosage*; D011485:Protein Binding; D013678:Technology, Pharmaceutical / Q000379:methods
D010455:Peptides; D004364:Pharmaceutical Preparations; D010761:Phosphorus Radioisotopes