Association between vitamin D receptor gene polymorphisms and susceptibility to chronic kidney disease and periodontitis.
OBJECTIVE
Chronic kidney disease (CKD) and periodontitis (PD) are serious public-health concerns. Vitamin D is a fat-soluble steroid hormone that interacts with its nuclear receptor (VDR) to regulate a variety of biological processes, such as bone metabolism, immune response modulation and transcription of several genes involved in CKD and PD disease mechanisms. The aim of this work was to investigate the association between polymorphisms in the VDR gene and end-stage renal disease (ESRD) and PD.


METHODS
222 subjects with and without ESRD (in hemodialysis) were divided into groups with and without PD. Polymorphisms TaqI and BsmI in the VDR gene were analyzed by PCR restriction fragment length polymorphism. The significance of differences in allele, genotype and haplotype frequencies between groups was assessed by the chi2 test (p value <0.05) and odds ratio (OR).


RESULTS
Allele G was associated with protection against ESRD: groups without versus with ESRD (GG) x (GA+AA): OR = 2.5, 95% CI = 1.4-4.6, p = 0.00; (G x A): OR = 1.5, 95% CI = 1.0-2.3, p = 0.02; (TG + CG) x (TA + CA): OR = 1.5, 95% CI = 1.0-2.3, p = 0.02. No association was observed between the study polymorphisms and susceptibility to or protection against PD.


CONCLUSIONS
Allele G of the VDR BsmI polymorphism was associated with protection against ESRD.
de Souza
Cleber Machado
CM
Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
Braosi
Ana Paula Ribeiro
AP
Luczyszyn
Sônia Mara
SM
Avila
Andréa Rodrigues
AR
de Brito
Rui Barbosa
RB
Ignácio
Sérgio Aparecido
SA
Probst
Christian Macagnan
CM
Riella
Miguel Carlos
MC
Sotomaior
Vanessa Santos
VS
Mira
Marcelo Távora
MT
Pecoits-Filho
Roberto
R
Trevilatto
Paula Cristina
PC
D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D015252:Deoxyribonucleases, Type II Site-Specific; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007676:Kidney Failure, Chronic / Q000209:etiology / Q000235:genetics*; D008875:Middle Aged; D010518:Periodontitis / Q000209:etiology / Q000235:genetics*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D018167:Receptors, Calcitriol / Q000235:genetics*
D018167:Receptors, Calcitriol; C053864:endodeoxyribonuclease BsmI; D015252:Deoxyribonucleases, Type II Site-Specific