Tobacco smoke-induced left ventricular remodelling is not associated with metalloproteinase-2 or -9 activation.
OBJECTIVE
To investigate the role of MMP-2 and MMP-9 in cardiac remodelling induced by tobacco smoke exposure in rats.


METHODS
Rats were allocated into two groups: C (n=9): control animals; ETS (n=9): exposed to tobacco smoke. After 4months, the animals underwent echocardiography, morphometric study and determination of MMP-2 and MMP-9 activity.


RESULTS
ETS rats had larger diastolic (C=15.6 +/-1.2 mm/kg, ETS=18.0+/-0.9 mm/kg; p < 0.001) and systolic (C=7.3+/-1.2 mm/kg, ETS=9.2+/-0.9 mm/kg; p=0.001) ventricular diameters adjusted for body weight. Fractional shortening (C=53+/-4.8%, ETS=48+/- 3.3%; p=0.031) and ejection fraction (C=0.89+/-0.03, ETS=0.86+/-0.02; p=0.030) were smaller in the ETS group. Myocyte cross-sectional area (C=245+/-8 microm2, ETS=253+/-8 microm2; p=0.028) was higher in ETS rats. There were no differences in MMP-2 (C=50+/-14%; ETS=43+/-11%, p=0.228) or MMP-9 (C=0.36+/-0.3%; ETS=0.62+/-0.3%, p=0.630) activity between the groups.


CONCLUSIONS
MMP-2 and MMP-9 did not participate in the remodelling process induced by tobacco smoke exposure.
Castardeli
Edson
E
Departamento de Clínica Médica, Faculdade de Medicina de Botucatu, UNESP - São Paulo State University, Botucatu, Brazil.
Duarte
Daniella R
DR
Minicucci
Marcos F
MF
Azevedo
Paula S
PS
Matsubara
Beatriz B
BB
Matsubara
Luiz S
LS
Campana
Alvaro O
AO
Paiva
Sergio A R
SA
Zornoff
Leonardo A M
LA
D000818:Animals; D004452:Echocardiography; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D018487:Ventricular Dysfunction, Left / Q000201:enzymology*; D020257:Ventricular Remodeling
D014028:Tobacco Smoke Pollution; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9