Effect of garlic supplementation on erythrocytes antioxidant parameters, lipid peroxidation, and atherosclerotic plaque formation process in oxidized oil-fed rabbits.
Effect of garlic supplementation on blood antioxidant status, lipid peroxidation, and coronary plaque formation process was investigated in oxidized oil-fed rabbits. Eighteen adult male mixed European rabbits were given a balanced diet (21 g% protein, 34 g% fat, 45 g% carbohydrate), which contained isocaloristic addition of nonoxidized or oxidized rapeseed oil in the presence and absence of garlic. The experiment lasted 24 weeks. At the beginning and every 6 weeks, rabbits were weighed, and blood was taken. To evaluate the antioxidant status of the rabbits, erythrocytes malondialdehyde (MDA) concentration, total superoxide dismutase (t-SOD), and glutathione peroxidase (GPX) activations were determined. After the experiment was completed, aortas were dissected for histological examinations. Changes in the contents of the above parameters and histological examinations showed that oxidized rapeseed, oil administered to rabbits, caused the development of atherosclerotic changes and disturbed antioxidant status. The addition of garlic in such diets inhibited atherosclerotic changes in the aorta wall, and it is related to the homeostatic activity of antioxidative enzymes and lipid peroxidation.
Zalejska-Fiolka
Jolanta
J
Department of Biochemistry in Zabrze, Medical University of Silesia, Katowice, Poland. jolafiolka@wp.pl
Kasperczyk
Aleksandra
A
Kasperczyk
Sławomir
S
Błaszczyk
Urszula
U
Birkner
Ewa
E
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D029741:Brassica rapa; D003324:Coronary Artery Disease / Q000517:prevention & control*; D004912:Erythrocytes / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated; D005737:Garlic; D005979:Glutathione Peroxidase / Q000097:blood; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D010084:Oxidation-Reduction; D010938:Plant Oils; D011817:Rabbits; D000074262:Rapeseed Oil; D013482:Superoxide Dismutase / Q000097:blood
D000975:Antioxidants; D005229:Fatty Acids, Monounsaturated; D010938:Plant Oils; D000074262:Rapeseed Oil; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase