title: [Palindromic sequence-mediated de novo chromosome translocation in sperm samples derived from normal and oligospermic males].
abstract: OBJECTIVE
  To systematically explore the occurrence of a novel type of chromosome translocation in human sperm samples.
  
  
  METHODS
  Specific translocation junction fragments were quantified using nested and/or multi-nested PCR in sperm DNA derived from 28 oligospermic patients and 32 normal controls.
  
  
  RESULTS
  t(11;22) was detected in 49 samples. At least 4 samples were found to have t(1;22) (p21.2;q11.2), t(17;22) (q11;q11) or t(X;22) (q27;q11). The mutation rate seemed to be associated not with age or semen volume, but with sperm concentration (r = -0.389, P < 0.05) and motility (r = -0.397, P < 0.05). Correlation was not found between homology of palindromic sequences and mutation rate.
  
  
  CONCLUSIONS
  Palindromic sequence mediated chromosome translocation is common in human sperm, and associated with sperm concentration and motility. Measurement of such mutations may provide a molecular-level reference for assessing sperm quality.
authors.affiliation: Department of Medical Genetics, China Medical University, Shenyang, Liaoning 110001, China.
authors.forename: Ti
authors.initials: T
authors.lastname: Wen
authors.forename: Jing-shu
authors.initials: JS
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Ci
authors.forename: Yan-hui
authors.initials: YH
authors.lastname: Zhao
authors.forename: Xiang
authors.initials: X
authors.lastname: Fei
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Ling
authors.initials: L
authors.lastname: Li
mesh_terms: D020856:AT Rich Sequence; D000328:Adult; D001483:Base Sequence; D002880:Chromosomes, Human, Pair 11; D002886:Chromosomes, Human, Pair 17; D002892:Chromosomes, Human, Pair 22; D041321:Chromosomes, Human, X; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009845:Oligospermia / Q000235:genetics*; D013094:Spermatozoa / Q000378:metabolism*; D014178:Translocation, Genetic