A novel biotransformation of 2-formyl-6-naphthoic acid to 2,6-naphthalene dicarboxylic acid by Pseudomonas sp. for the purification of crude 2,6-naphthalene dicarboxylic acid.
Crude 2,6-naphthalene dicarboxylic acid was purified by Pseudomonas sp. HN-72 which biotransformed the major impurity of 2-formyl-6-naphthoic acid into 2,6-naphthalene dicarboxylic acid. The biotransformation yield reached 100% when the reaction was performed at 40 degrees C for 1 h, in 200 ml KH(2)PO(4)/KOH buffer (50 mM, pH 8.0), with 0.2% (w/v) crude 2,6-naphthalene dicarboxylic acid and 2.5 mg dry cell wt/ml.
Kim
Dong-Sung
DS
R&D Business Labs, Hyosung Corporation, Anyang-Si, Gyeonggi-Do 431-080, Korea. ehdtjd9233@hanmail.net
Kim
Sung-Kyoon
SK
Choi
Yong-Bok
YB
Kwon
Ik-Hyeon
IH
Park
Kwan-Hwa
KH
D002264:Carboxylic Acids / Q000737:chemistry*; D003998:Dicarboxylic Acids / Q000737:chemistry* / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry; D009281:Naphthalenes / Q000737:chemistry* / Q000302:isolation & purification*; D011549:Pseudomonas / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*
C528312:2-formyl-6-naphthoic acid; D002264:Carboxylic Acids; D003998:Dicarboxylic Acids; D009281:Naphthalenes; C109064:2,6-naphthalenedicarboxylic acid