title: A key role for the Arabidopsis WIN3 protein in disease resistance triggered by Pseudomonas syringae that secrete AvrRpt2.
abstract: Effector proteins injected by the pathogenic bacteria Pseudomonas syringae into plants can have profound effects on the pathogen-host interaction due to their efficient recognition by plants and the subsequent triggering of defenses. The AvrRpt2 effector triggers strong local and systemic defense (called systemic acquired resistance [SAR]) responses in Arabidopsis thaliana plants that harbor a functional RPS2 gene that encodes an R protein in the coiled-coil, nucleotide-binding domain, leucine-rich repeat class. The newly identified win3-T mutant shows greatly reduced resistance to P syringae carrying avrRpt2. In win3-T plants, RIN4 cleavage, an early AvrRpt2-induced event, is normal. However, salicylic acid accumulation is compromised, as is SAR induction and the local hypersensitive cell death response after infection by P syringae carrying avrRpt2. WIN3 encodes a member of the firefly luciferase protein superfamily. Expression of WIN3 at an infection site partially requires PAD4, a protein known to play a quantitative role in RPS2-mediated signaling. WIN3 expression in tissue distal to an infection site requires multiple salicylic acid regulatory genes. Finally, win3-T plants show modestly increased susceptibility to virulent P syringae and modestly reduced SAR in response to P. syringae carrying avrRpm1. Thus, WIN3 is a key element of the RPS2 defense response pathway and a basal and systemic defense component.
authors.affiliation: Molecular Genetics and Cell Biology Department, The University of Chicago, 1103 East 57th Street, Chicago, IL 60637, USA.
authors.forename: Min Woo
authors.initials: MW
authors.lastname: Lee
authors.forename: Hua
authors.initials: H
authors.lastname: Lu
authors.forename: Ho Won
authors.initials: HW
authors.lastname: Jung
authors.forename: Jean T
authors.initials: JT
authors.lastname: Greenberg
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D007113:Immunity, Innate; D047908:Intracellular Signaling Peptides and Proteins; D049409:Luciferases, Firefly / Q000235:genetics / Q000494:pharmacology*; D008954:Models, Biological; D010935:Plant Diseases; D044224:Pseudomonas syringae / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D049409:Luciferases, Firefly; C502082:AvrRpm1 protein, Pseudomonas syringae; C453279:RIN4 protein, Arabidopsis; C089426:RPS2 protein, Arabidopsis; C420919:avrRpt2 protein, Pseudomonas syringae; C523087:At5g13320 protein, Arabidopsis