title: [Optimization of extraction parameters and de-protein process for Gastrodia elata. Polysaccharides].
abstract: OBJECTIVE
  Grope for Optimization of Extraction Parameters and De-protein Process for Gastrodia elata Bl. Polysaccharides (GPS). Provide the theoretical basis for the industrialization production of GPS.
  
  
  METHODS
  The effects of extracting temperature, ration between Gastrodia elata Bl. and water, heating time on extraction rate of GPS were studied with orthogonal experimental design. Compared the application of the two de-protein process measures (Sevag and Enzyme-Sevag) in purification technics for Gastrodia elata Bl.
  
  
  RESULTS
  The optimum parameters were: extraction 3h at 120 degrees C with 1:40 ratio of Gastrodia elata Bl. to water. The de-protein efficiency of Enzyme-Sevag is better than Sevag.
authors.affiliation: Southwest University of Science and Technology, Mianyang 621010, China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Zhu
authors.forename: Xue-Gang
authors.initials: XG
authors.lastname: Luo
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D031668:Gastrodia / Q000737:chemistry*; D035281:Plant Tubers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D011134:Polysaccharides