title: Prevotella nanceiensis sp. nov., isolated from human clinical samples.
abstract: Three strains of anaerobic, non-pigmented, Gram-negative bacilli isolated from various human clinical samples were characterized in terms of phenotypic and genotypic tests, including sequence analysis of 16S rRNA and rpoB genes. The strains were most closely related to the type strains of Prevotella marshii and Prevotella shahii on the basis of both 16S rRNA (89.8 and 89.0 % identity, respectively) and rpoB gene sequences (83.1 and 82.8 % identity, respectively). Phylogenetic analysis showed that the isolates constituted a robust homogeneous group distinct from known species in the genus Prevotella. The rrn skeleton (as determined by PFGE) and the DNA G+C content, determined to be 39.4 mol% for strain LBN 293(T), distinguished the novel isolates from the type strains of P. marshii and P. shahii. The three strains were saccharolytic and produced acetic, lactic and succinic acids as major metabolic end products. Polyphasic investigations supported the proposal of a novel species, Prevotella nanceiensis sp. nov., with LBN 293(T) (=AIP 261.03(T) =CIP 108993(T) =CCUG 54409(T)) as the type strain.
authors.affiliation: Centre Hospitalier et Universitaire de Nancy, Hôpital Central, Laboratoire de Bactériologie, Nancy, France.|Université Nancy I, UMR CNRS 7565, UFR de Médecine, Laboratoire de Bactériologie, Vandoeuvre-les-Nancy, France.
authors.forename: C
authors.initials: C
authors.lastname: Alauzet
authors.affiliation: Centre Hospitalier et Universitaire de Nancy, Hôpital Central, Laboratoire de Bactériologie, Nancy, France.
authors.forename: F
authors.initials: F
authors.lastname: Mory
authors.affiliation: Institut Pasteur, Centre National de Référence des Bactéries Anaérobies et du Botulisme, Paris, France.
authors.forename: J-P
authors.initials: JP
authors.lastname: Carlier
authors.affiliation: Université Montpellier 1, UFR de Pharmacie, Laboratoire de Bactériologie-Virologie, EA 3755, Montpellier, France.|Centre Hospitalier et Universitaire de Montpellier, Hôpital Arnaud de Villeneuve, Laboratoire de Bactériologie, Montpellier, France.
authors.forename: H
authors.initials: H
authors.lastname: Marchandin
authors.affiliation: Université Montpellier 1, UFR de Pharmacie, Laboratoire de Bactériologie-Virologie, EA 3755, Montpellier, France.
authors.forename: E
authors.initials: E
authors.lastname: Jumas-Bilak
authors.affiliation: Centre Hospitalier et Universitaire de Nancy, Hôpital Central, Laboratoire de Bactériologie, Nancy, France.|Université Nancy I, UMR CNRS 7565, UFR de Médecine, Laboratoire de Bactériologie, Vandoeuvre-les-Nancy, France.
authors.forename: A
authors.initials: A
authors.lastname: Lozniewski
mesh_terms: D000693:Anaerobiosis / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D016866:Bacteroidaceae Infections / Q000382:microbiology*; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D020459:Genes, rRNA; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D018720:Prevotella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010860:Pigments, Biological; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit