Induction of DAN/TIR yeast cell wall mannoprotein genes in response to high hydrostatic pressure and low temperature.
Global transcriptional profiles of Saccharomyces cerevisiae were studied following changes in growth conditions to high hydrostatic pressure and low temperature. These profiles were quantitatively very similar, encompassing 561 co-upregulated genes and 161 co-downregulated genes. In particular, expression of the DAN/TIR cell wall mannoprotein genes, which are generally expressed under hypoxia, were markedly upregulated by high pressure and low temperature, suggesting the overlapping regulatory networks of transcription. In support of the role of mannoproteins in cell wall integrity, cells acquired resistance against treatment with SDS, Zymolyase and lethal levels of high pressure when preincubated under high pressure and low temperature.
Abe
Fumiyoshi
F
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan. abef@jamstec.go.jp
D002473:Cell Wall / Q000187:drug effects; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D006867:Hydrolases / Q000494:pharmacology; D006874:Hydrostatic Pressure; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013696:Temperature; D015854:Up-Regulation
D008562:Membrane Glycoproteins; D029701:Saccharomyces cerevisiae Proteins; C025363:mannoproteins; D012967:Sodium Dodecyl Sulfate; C037833:zymolyase; D006867:Hydrolases