title: Transcriptional profiling of inductive mesenchyme to identify molecules involved in prostate development and disease.
abstract: BACKGROUND
  The mesenchymal compartment plays a key role in organogenesis, and cells within the mesenchyme/stroma are a source of potent molecules that control epithelia during development and tumorigenesis. We used serial analysis of gene expression (SAGE) to profile a key subset of prostatic mesenchyme that regulates prostate development and is enriched for growth-regulatory molecules.
  
  
  RESULTS
  SAGE libraries were constructed from prostatic inductive mesenchyme and from the complete prostatic rudiment (including inductive mesenchyme, epithelium, and smooth muscle). By comparing these two SAGE libraries, we generated a list of 219 transcripts that were enriched or specific to inductive mesenchyme and that may act as mesenchymal regulators of organogenesis and tumorigenesis. We identified Scube1 as enriched in inductive mesenchyme from the list of 219 transcripts; also, quantitative RT-PCR and whole-mount in situ hybridization revealed Scube1 to exhibit a highly restricted expression pattern. The expression of Scube1 in a subset of mesenchymal cells suggests a role in prostatic induction and branching morphogenesis. Additionally, Scube1 transcripts were expressed in prostate cancer stromal cells, and were less abundant in cancer associated fibroblasts relative to matched normal prostate fibroblasts.
  
  
  CONCLUSIONS
  The use of a precisely defined subset of cells and a back-comparison approach allowed us to identify rare mRNAs that could be overlooked using other approaches. We propose that Scube1 encodes a novel stromal molecule that is involved in prostate development and tumorigenesis.
authors.affiliation: MRC Human Reproductive Sciences Unit, The Queens Medical Research Institute, Little France Crescent, Edinburgh EH16 4TJ, UK. g.vanpoucke@hrsu.mrc.ac.uk
authors.forename: Griet
authors.initials: G
authors.lastname: Vanpoucke
authors.forename: Brigid
authors.initials: B
authors.lastname: Orr
authors.forename: O Cathal
authors.initials: OC
authors.lastname: Grace
authors.forename: Ray
authors.initials: R
authors.lastname: Chan
authors.forename: George R
authors.initials: GR
authors.lastname: Ashley
authors.forename: Karin
authors.initials: K
authors.lastname: Williams
authors.forename: Omar E
authors.initials: OE
authors.lastname: Franco
authors.forename: Simon W
authors.initials: SW
authors.lastname: Hayward
authors.forename: Axel A
authors.initials: AA
authors.lastname: Thomson
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D017403:In Situ Hybridization; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008648:Mesoderm / Q000378:metabolism*; D011467:Prostate / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; C528568:Scube1 protein, rat