Ontogeny of leptin and its receptor expression in mouse testis during the postnatal period.
The mechanism by which leptin regulate male reproductive development during postnatal periods remain to be determined. Using immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR), we established that leptin is expressed in mouse testis with a cell-type and stage-dependent manner during the postnatal period. In testes of 5-day-old mice, leptin expression was mainly restricted to gonocytes, whereas the immunostaining of leptin was confined to spermatogonia in 10-day-old testes. From Day 20 and onwards, stage-specific expression of leptin was evident in spermatocytes at stages VII-XII of the cycle in the seminiferous epithelium. RT-PCR showed that leptin and its receptor isoforms, Ob-Ra, Ob-Rb, and Ob-Re were all expressed in testes from 5- to 60-day-old mice. The mRNA for Ob-Ra and Ob-Re, but not Ob-Rb or leptin were identified in both immature (14-day-old) and adult (60-day-old) isolated Leydig cells. These results suggest that besides its primary actions at the hypothalamic-pituitary level, leptin has direct effects in the proliferation, differentiation of germ cells and the modulation of testicular steroidogenesis, using both autocrine and paracrine mechanisms.
Herrid
Muren
M
Animal Physiology, University of New England, Armidale, New South Wales, Australia. Muren.Herrid@csiro.au
O'Shea
Tim
T
McFarlane
James R
JR
D000818:Animals; D019898:Autocrine Communication / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D005786:Gene Expression Regulation / Q000502:physiology*; D007030:Hypothalamo-Hypophyseal System / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D007150:Immunohistochemistry; D020738:Leptin / Q000096:biosynthesis*; D007985:Leydig Cells / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D019899:Paracrine Communication / Q000502:physiology; D020033:Protein Isoforms / Q000096:biosynthesis; D054411:Receptors, Leptin / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012670:Seminiferous Epithelium / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D013090:Spermatocytes / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D013093:Spermatogonia / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*
D020738:Leptin; D020033:Protein Isoforms; D054411:Receptors, Leptin; C515447:leptin receptor, mouse