Determination of phenolic content and antioxidant activity of extracts obtained from Rosmarinus officinalis' calli.
Rosmarinus officinalis is widely found in the lands of Aegean and Mediterranean regions of Turkey. Stem explants of very young shoots were cultured in both woody plant medium (WPM) and Murashige and Skoog (MS) media supplemented with 7g/L agar, 30g/L sucrose, and 1 and 3mg/L naphthaleneacetic acid (NAA) for callus initiation. Induced calli were subcultured 4 times with intervals of 7-10 days. MS medium supplemented with 1mg/L NAA proved to be the best medium for the production of callus (65.0%) among the samples tested. The lyophilized calli were subjected to solvent extraction. Active constituents of 8 calli extracts were analyzed by HPLC, and rosmarinic acid (RA) was determined to be the primary compound. Calli cultivated in WPM supplemented with 1mg/L NAA and extracted at 50 degrees C, yielded the highest amount of RA (34.4mg/g dry weight). Moreover, antioxidant activity of calli extracts was determined using a number of in vitro assays, including total phenol assay, DPPH radical scavenging activity (RSA), and trolox equivalent antioxidant capacity (TEAC). On the basis of the current findings, we conclude that WPM supplemented with 1mg/L NAA yields higher phenolic content as well as higher antioxidant activity.
Yesil-Celiktas
Ozlem
O
Ege University Science and Technology Center, 35100 Bornova-Izmir, Turkey. ozlem.yesil.celiktas@ege.edu.tr
Nartop
Pinar
P
Gurel
Aynur
A
Bedir
Erdal
E
Vardar-Sukan
Fazilet
F
D000975:Antioxidants / Q000737:chemistry*; D001713:Biphenyl Compounds; D002839:Chromans; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006834:Hydrazines; D010636:Phenols / Q000737:chemistry*; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D027542:Rosmarinus / Q000737:chemistry*; D013997:Time Factors; D046509:Tissue Culture Techniques
D000975:Antioxidants; D001713:Biphenyl Compounds; D002839:Chromans; D016166:Free Radical Scavengers; D006834:Hydrazines; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid