Promotion of energy transfer and oxygen evolution in spinach photosystem II by nano-anatase TiO2.
Being a proven photocatalyst, nano-anatase is capable of undergoing electron transfer reactions under light. In previous studies we had proven that nano-anatase improved photosynthesis and greatly promoted spinach growth. The mechanisms by which nano-anatase promotes energy transfer and the conversion efficiency of the process are still not clearly understood. In the present paper, we report the results obtained with the photosystem II (PSII) isolated from spinach and treated by nano-anatase TiO2 and studied the effect of nano-anatase TiO2 on energy transfer in PSII by spectroscopy and on oxygen evolution. The results showed that nano-anatase TiO2 treatment at a suitable concentration could significantly change PSII microenvironment and increase absorbance for visible light, improve energy transfer among amino acids within PSII protein complex, and accelerate energy transport from tyrosine residue to chlorophyll a. The photochemical activity of PSII (fluorescence quantum yield) and its oxygen-evolving rate were enhanced by nano-anatase TiO2. This is viewed as evidence that nano-anatase TiO2 can promote energy transfer and oxygen evolution in PSII of spinach.
Su
Mingyu
M
College of Life Science, Suzhou University, Suzhou 215123, People's Republic of China.
Mingyu
Su
S
Wu
Xiao
X
Xiao
Wu
W
Liu
Chao
C
Chao
Liu
L
Qu
Chunxiang
C
Chunxiang
Qu
Q
Liu
Xiaoqing
X
Xiaoqing
Liu
L
Chen
Liang
L
Liang
Chen
C
Huang
Hao
H
Hao
Huang
H
Hong
Fashui
F
Fashui
Hong
H
D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D004735:Energy Transfer / Q000187:drug effects*; D010100:Oxygen / Q000378:metabolism*; D045332:Photosystem II Protein Complex / Q000187:drug effects* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D018724:Spinacia oleracea; D014025:Titanium / Q000494:pharmacology*
D045332:Photosystem II Protein Complex; D002734:Chlorophyll; C009495:titanium dioxide; D014025:Titanium; D010100:Oxygen; D000077194:Chlorophyll A