[Correlation of Epstein-Barr virus A73 gene polymorphisms to susceptibility to nasopharyngeal carcinoma].
OBJECTIVE
A73 gene, one of the primary members in Epstein-Barr virus (EBV) BamHI-A rightward transcripts (BARTs) family, is related to the development of nasopharyngeal carcinoma (NPC). Its mRNA level is elevated in NPC tissues, suggesting that A73 participates in the carcinogenesis of NPC. This study was to explore the polymorphism loci of A73 gene and find its correlation to susceptibility to NPC.


METHODS
Nested polymerase chain reaction (PCR) and direct sequencing were used to identify A73 gene polymorphisms in 23 specimens of NPC and 19 throat washing (TW) samples from non-NPC patients. The same methods were also used on additional 54 unitized NPC specimens [carcinoma tissue, matched TW sample and peripheral blood (PB) sample from the same patient], and 48 TW samples and 51 PB samples from healthy Cantonese to detect a polymorphism with high frequency found in previous step. Its distribution among different samples was compared. All sequences were compared with the sequence of B95.8 cells.


RESULTS
Three polymorphisms A157154C, G159188C, and G159209C were identified in A73 gene. Among which, A157154C showed high frequency. The frequency of A157154C was significant higher in PB samples from NPC patients than in those from healthy Cantonese (96.29% vs. 64.70%, P<0.001). Among 54 unitized specimens, 13 cases showed discordances in different tissue origins.


CONCLUSIONS
A157154C in A73 gene might be correlated to the occurrence and development of NPC.
Zhang
Lin-Lin
LL
State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, 510060, P. R. China.
Li
Da-Jiang
DJ
Li
Zhi-Heng
ZH
Zhang
Xiao-Shi
XS
Zhang
Ru-Hua
RH
Yu
Xing-Juan
XJ
Chen
Li-Zhen
LZ
Feng
Qi-Sheng
QS
Zeng
Yi-Xin
YX
Jia
Wei-Hua
WH
D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D005091:Exons; D005787:Gene Frequency; D005814:Genes, Viral; D020022:Genetic Predisposition to Disease; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000821:virology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D004279:DNA, Viral