Preparation of rapamycin-loaded chitosan/PLA nanoparticles for immunosuppression in corneal transplantation.
Rapamycin-loaded chitosan/polylactic acid nanoparticles with size of about 300 nm in diameter were prepared through nanoprecipitation method using cholesterol-modified chitosan as a stabilizer. The surface coating of chitosan, which was demonstrated by zeta potential measurement, endowed the nanoparticles good retention ability at the procorneal area, facilitating the sustained release of rapamycin on the corneal. The immunosuppression in corneal transplantation of the nanoparticles was investigated using rabbit as animal model, the median survival time of the corneal allografts treated with nanoparticles was 27.2+/-1.03 days and 50% grafts still remained surviving by the end of the observation, while the group treated with 0.5% rapamycin suspension was 23.7+/-3.20 days. The median survival time of drug-free nanoparticles group and untreated groups were 10.9+/-1.45 and 10.6+/-1.26 days, respectively. The results demonstrated the excellent immunosuppression of rapamycin-loaded chitosan/polylactic acid nanoparticles in corneal transplantation.
Yuan
Xu-Bo
XB
School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China. xbyuan@tju.edu.cn
Yuan
Yan-Bo
YB
Jiang
Wei
W
Liu
Jie
J
Tian
En-Jiang
EJ
Shun
Hui-Ming
HM
Huang
Ding-Hai
DH
Yuan
Xiao-Yan
XY
Li
Hong
H
Sheng
Jing
J
D000287:Administration, Topical; D000818:Animals; D002626:Chemistry, Pharmaceutical; D048271:Chitosan; D002784:Cholesterol; D016039:Corneal Transplantation / Q000276:immunology*; D004337:Drug Carriers; D004563:Electrochemistry; D007166:Immunosuppressive Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D007553:Isotope Labeling; D019344:Lactic Acid; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D009883:Ophthalmic Solutions; D011091:Polyesters; D011108:Polymers; D011817:Rabbits; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D020123:Sirolimus / Q000008:administration & dosage* / Q000494:pharmacology*; D012995:Solubility; D016284:Technetium Tc 99m Pentetate
D004337:Drug Carriers; D007166:Immunosuppressive Agents; D009883:Ophthalmic Solutions; D011091:Polyesters; D011108:Polymers; D019275:Radiopharmaceuticals; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan; D002784:Cholesterol; D016284:Technetium Tc 99m Pentetate; D020123:Sirolimus