title: In situ imaging of the endogenous CD8 T cell response to infection.
abstract: Mounting a protective immune response is critically dependent on the orchestrated movement of cells within lymphoid organs. We report here the visualization, using major histocompatability complex class I tetramers, of the CD8-positive (CD8) T cell response in the spleens of mice to Listeria monocytogenes infection. A multistage pathway was revealed that included initial activation at the borders of the B and T cell zones followed by cluster formation with antigenpresenting cells leading to CD8 T cell exit to the red pulp via bridging channels. Strikingly, many memory CD8 T cells localized to the B cell zones and, when challenged, underwent rapid migration to the T cell zones where proliferation occurred, followed by egress via bridging channels in parallel with the primary response. Thus, the ability to track endogenous immune responses has uncovered both distinct and overlapping mechanisms and anatomical locations driving primary and secondary immune responses.
authors.affiliation: Department of Immunology, University of Connecticut, Farmington, CT 06030, U.S.A.
authors.forename: Kamal M
authors.initials: KM
authors.lastname: Khanna
authors.forename: Jeffery T
authors.initials: JT
authors.lastname: McNamara
authors.forename: Leo
authors.initials: L
authors.lastname: Lefrançois
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology; D016827:CD8 Antigens / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D002465:Cell Movement; D003713:Dendritic Cells / Q000276:immunology / Q000502:physiology; D005456:Fluorescent Dyes; D015395:Histocompatibility Antigens Class I; D007156:Immunologic Memory; D007700:Kinetics; D008089:Listeria monocytogenes / Q000276:immunology*; D008088:Listeriosis / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013194:Staining and Labeling; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology / Q000502:physiology
chemical_list: D015703:Antigens, CD; D016827:CD8 Antigens; D005456:Fluorescent Dyes; D015395:Histocompatibility Antigens Class I; D011948:Receptors, Antigen, T-Cell