Enzymatic modification of saponins from Platycodon grandiflorum with Aspergillus niger.
Platycodon grandiflorum A. DC (Campanulaceae) is a traditional medicinal plant. Its root, Platycodi Radix, contains an abundant amount of saponin glycosides, platycodins, of which platycodin D is one of the major components. The chemical structures of platycodins can be modified by various types of chemical processing, but a modification mediated with microorganisms has been not reported yet. In this study, platycodin D was modified to several partially degraded platycodin glycosides after treatment with a crude enzyme extract from Aspergillus niger (A. niger). The modified platycodin D possessed a shorter sugar side-chain, and presented a remarkably reduced V79-4 cell (Chinese hamster lung fibroblasts) cytotoxicity and erythrocyte hemolytic toxicity, whereas the nitrite-scavenging activity was increased in the modified platycodin D. Sensory scores for pungency, bitterness and after-taste were improved as well in the modified platycodin D. Results suggest that A. niger mediated modification yielded a novel partially degraded platycodin glycoside which possesses increased bioactivities and improved sensory values, yet with reduced toxic profiles.
Wie
Hye Jeong
HJ
Department of Food and Nutrition, Seoul National University, Shillim-dong, Kwanak-ku, Seoul 151, Korea.
Zhao
Hai Lin
HL
Chang
Jeong Hwa
JH
Kim
Yeong Shik
YS
Hwang
In Kyeong
IK
Ji
Geun Eog
GE
D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D006026:Glycoside Hydrolases / Q000378:metabolism; D006461:Hemolysis; D051379:Mice; D008813:Mice, Inbred ICR; D031238:Platycodon / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000378:metabolism* / Q000494:pharmacology; D014315:Triterpenes / Q000378:metabolism / Q000494:pharmacology
D012503:Saponins; D014315:Triterpenes; C108953:platycodin D; D006026:Glycoside Hydrolases