title: Exposure to extremely low frequency magnetic fields affects insulin-secreting cells.
abstract: To evaluate the effects of extremely low frequency magnetic field (ELFMF) on beta-cell survival and function, we cultured a hamster-derived insulin-secreting cell line (HIT-T15), which exhibits responsiveness to glucose in a semi-physiological range, under exposure to sham and ELFMF conditions, and assessed cell survival and function. We used our previously developed ELFMF exposure unit (a sinusoidal magnetic field at a frequency of 60 Hz, 5 mT) to culture cells under exposure to ELFMF conditions. We found that exposure to ELFMF for 5 days in the absence of glucose increased cell number, exposure for 2 days in the absence of glucose and for 5 days with 100 mg/dl glucose increased the insulin secretion to the culture medium, and exposure for 2 and 5 days with 40 and 100 mg/dl glucose increased intracellular insulin concentration in HIT-T15 cells. The increase in cell number under apoptotic culture conditions by exposure to ELFMF could lead to new therapeutic concepts in the treatment of diabetes. The ELFMF-induced increase in intracellular insulin concentration could be utilized to develop culture conditions to enhance intracellular insulin concentration in insulin-secreting cells that would be useful for cell transplantation to cure diabetes mellitus.
authors.affiliation: Department of Radiological Technology, School of Health Sciences, Faculty of Medicine, Hirosaki University, Hirosaki, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Sakurai
authors.forename: Miwa
authors.initials: M
authors.lastname: Yoshimoto
authors.forename: Shin
authors.initials: S
authors.lastname: Koyama
authors.forename: Junji
authors.initials: J
authors.lastname: Miyakoshi
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D006224:Cricetinae; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D011829:Radiation Dosage
chemical_list: D007328:Insulin; D005947:Glucose