title: An association between parvovirus B19 and Kikuchi-Fujimoto disease.
abstract: Although Kikuchi-Fujimoto disease (KFD) has a higher prevalence among Asian countries, it is a well-defined entity throughout the world. However, its etiology and pathogenesis remain undetermined. To study whether B19 infection is associated with idiopathic KFD (iKFD), we examined the presence of the viral genome and proteins in paraffin-embedded tissues of lymph nodes retrospectively from 33 iKFD patients and 16 age- and sex-matched control subjects by nested PCR (nPCR), in situ hybridization (ISH), and immunohistochemistry (IHC). B19 was detected in 87.1, 69.7, and 57.6% of iKFD specimens by nPCR, ISH, and IHC, respectively, whereas the virus was positive in only 56.3, 31.3, and 25.0% of control tissues by the respective methods (nPCR: p = 0.029; ISH: p = 0.011; IHC: p = 0.032). The IHC-ISH double-staining assay demonstrated that B19-infected cells were mainly lymphocytes and a small number of histiocytes. These results showed for the first time a high frequency of localized persistence of B19 in lymph nodes from iKFD patients, suggesting that B19 might play an important role in the pathogenesis of iKFD.
authors.affiliation: Department of Pathology and State Key Laboratory of Cancer Biology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, People's Republic of China.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Zhang
authors.forename: Juan-Hong
authors.initials: JH
authors.lastname: Wang
authors.forename: Wen-Qing
authors.initials: WQ
authors.lastname: Wang
authors.forename: Xie-Qun
authors.initials: XQ
authors.lastname: Chen
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Yuan-Fei
authors.initials: YF
authors.lastname: Li
authors.forename: Pei-Zhen
authors.initials: PZ
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.forename: Di
authors.initials: D
authors.lastname: Wang
authors.forename: Gao-Sheng
authors.initials: GS
authors.lastname: Huang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006644:Histiocytes / Q000821:virology; D020042:Histiocytic Necrotizing Lymphadenitis / Q000209:etiology* / Q000821:virology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008198:Lymph Nodes / Q000821:virology; D008214:Lymphocytes / Q000821:virology; D008297:Male; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000821:virology*; D016732:Parvovirus B19, Human; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies
chemical_list: D004279:DNA, Viral