title: Urinary biomarkers of aflatoxin exposure in young children from Egypt and Guinea.
abstract: Aflatoxins are a major risk factor for hepatocellular carcinoma (HCC), and thus understanding the pattern of aflatoxin exposure in different regions is important in order to develop targeted intervention strategies. Given the early onset of HCC in many countries early life exposures may be important. This study investigated aflatoxin exposure in Egyptian children (n=50, aged 1-2.5 years) by assessing urinary aflatoxin metabolite (AFM(1), AFB(1), AFB(2), AFG(1), AFG(2)) levels. Samples from Guinean children (n=50, aged 2-4 years) were analyzed in parallel providing a comparison to a region of established frequent aflatoxin exposure. Aflatoxins were isolated from urine using C18-cartridges followed by immunoaffinity clean-up, and quantified by HPLC with fluorescence detection. Overall aflatoxins were less frequently present in Egyptian (38%) than Guinean urine samples (86%) (p<0.001), which was particularly related to differences in detection rates of AFM(1) (8% compared to 64%, respectively, (p<0.001)). For AFM(1) the geometric mean level in Guinea (16.3 pg/ml; 95% CI: 10.1, 26.6 pg/ml) was 6-fold higher (p<0.001) than in Egypt (2.7 pg/ml; 95% CI: 2.5, 2.8 pg/ml). Urinary aflatoxins from healthy children in these two regions have not previously been reported, and exposure appears modest in Egypt compared to Guinea. These data suggest that measures to reduce aflatoxin exposure in both regions are important, though particularly in Guinea.
authors.affiliation: Department of Clinical Nutrition, University of Kuopio, Finland. nektaria.polychronaki@uku.fi
authors.forename: Nektaria
authors.initials: N
authors.lastname: Polychronaki
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Wild
authors.forename: Hannu
authors.initials: H
authors.lastname: Mykkänen
authors.forename: Hassan
authors.initials: H
authors.lastname: Amra
authors.forename: Mosaad
authors.initials: M
authors.lastname: Abdel-Wahhab
authors.forename: Abdoulaye
authors.initials: A
authors.lastname: Sylla
authors.forename: Mamadou
authors.initials: M
authors.lastname: Diallo
authors.forename: Hani
authors.initials: H
authors.lastname: El-Nezami
authors.forename: Paul C
authors.initials: PC
authors.lastname: Turner
mesh_terms: D000348:Aflatoxins / Q000378:metabolism / Q000652:urine*; D015415:Biomarkers; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D003430:Cross-Sectional Studies; D004534:Egypt; D005260:Female; D016701:Guinea; D006515:Hepatitis B virus / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D008297:Male; D008895:Milk, Human / Q000737:chemistry*; D011042:Poisons / Q000378:metabolism / Q000652:urine*; D012423:Rural Health
chemical_list: D000348:Aflatoxins; D015415:Biomarkers; D011042:Poisons