title: Absolute configuration of tropane alkaloids bearing two alpha,beta-unsaturated ester functions using electronic CD spectroscopy: application to (R,R)-trans-3-hydroxysenecioyloxy-6-senecioyloxytropane.
abstract: The absolute configuration of heterocyclic natural products substituted with two mobile alpha,beta-unsaturated esters was studied using electronic circular dichroism (CD) spectroscopy. The conformational flexibility of the side chains imposed the use of density functional theory calculation to determine the set of the most probable conformations in solution. The electronic CD and UV spectra were calculated by Boltzmann-weighted average of the simulated spectra using the results of the excited states calculation of a set of simplified structures. Comparison with the experimental CD spectrum allowed to determine whether the calculations were made with the right enantiomer. The method was applied to the determination of the absolute configuration of (R,R)-trans-3-hydroxysenecioyloxy-6-senecioyloxytropane.
authors.affiliation: Laboratory of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva 4, Switzerland.
authors.forename: Munir
authors.initials: M
authors.lastname: Humam
authors.forename: Philippe
authors.initials: P
authors.lastname: Christen
authors.forename: Orlando
authors.initials: O
authors.lastname: Muñoz
authors.forename: Kurt
authors.initials: K
authors.lastname: Hostettmann
authors.forename: Damien
authors.initials: D
authors.lastname: Jeannerat
mesh_terms: D002942:Circular Dichroism; D004952:Esters; D008968:Molecular Conformation; D019657:Solanaceae / Q000737:chemistry; D013237:Stereoisomerism; D014326:Tropanes / Q000737:chemistry*
chemical_list: D004952:Esters; D014326:Tropanes