title: Effect of low dose antioxidant vitamin and trace element supplementation on the urinary concentrations of thromboxane and prostacyclin metabolites.
abstract: OBJECTIVE
  This trial evaluated the effect of antioxidant supplementation on the urinary excretion of 11-dehydro TXB(2)/2,3 dinor 6 keto PGF(1alpha) ratio, a marker of the pathogenesis of thrombosis and arteriosclerosis.
  
  
  METHODS
  This study was a randomised, double-blind, placebo-controlled trial involving 186 presumably healthy volunteers. One hundred received a multi-antioxidant supplementation and 86 a placebo for two years. Blood zinc, selenium, beta-carotene, vitamin C and E and urinary excretion of 11-dehydro TXB(2) and 2,3 dinor 6 keto PGF(1alpha) were measured.
  
  
  RESULTS
  Baseline subject characteristics did not differ between the two groups. Blood zinc, selenium, and beta-carotene concentrations significantly increased between baseline and two years in the multi-antioxidant supplementation group supporting subject compliance (p < 0.05). At two years, the median urinary 11-dehydro TXB(2)/2,3 dinor 6 keto PGF(1alpha) ratio was significantly lower in the multi-antioxidant supplementation group (3.4 versus 2.78, p = 0.015). Serum selenium concentration was the only antioxidant studied that was significantly related to the urinary 11-dehydro TXB(2)/2,3 dinor 6 keto PGF(1alpha) ratio.
  
  
  CONCLUSIONS
  These results support the hypothesis that a low-dose multi-antioxidant supplementation may contributes to a reduction in platelet activation which is beneficial for cardiovascular function.
authors.affiliation: Département de Biologie Intégrée, CHU de Grenoble, BP 217, 38043 Grenoble cedex 9, France. JArnaud@chu-grenoble.fr
authors.forename: Josiane
authors.initials: J
authors.lastname: Arnaud
authors.forename: Muriel
authors.initials: M
authors.lastname: Bost
authors.forename: Dominique
authors.initials: D
authors.lastname: Vitoux
authors.forename: José
authors.initials: J
authors.lastname: Labarère
authors.forename: Pilar
authors.initials: P
authors.lastname: Galan
authors.forename: Henri
authors.initials: H
authors.lastname: Faure
authors.forename: Serge
authors.initials: S
authors.lastname: Hercberg
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Bordet
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Roussel
authors.forename: Philippe
authors.initials: P
authors.lastname: Chappuis
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000031:analogs & derivatives / Q000652:urine; D000975:Antioxidants / Q000008:administration & dosage / Q000378:metabolism*; D001161:Arteriosclerosis / Q000097:blood / Q000517:prevention & control; D015415:Biomarkers / Q000652:urine; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010349:Patient Compliance; D015539:Platelet Activation / Q000187:drug effects; D044062:Prostaglandins I / Q000652:urine*; D012643:Selenium / Q000008:administration & dosage / Q000097:blood; D013927:Thrombosis / Q000097:blood / Q000517:prevention & control; D013929:Thromboxane B2 / Q000031:analogs & derivatives / Q000652:urine; D013931:Thromboxanes / Q000652:urine*; D014131:Trace Elements / Q000008:administration & dosage / Q000378:metabolism*; D014815:Vitamins / Q000008:administration & dosage / Q000378:metabolism*; D015032:Zinc / Q000008:administration & dosage / Q000097:blood; D019207:beta Carotene / Q000008:administration & dosage / Q000097:blood
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D044062:Prostaglandins I; D013931:Thromboxanes; D014131:Trace Elements; D014815:Vitamins; D019207:beta Carotene; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; C028290:2,3-dinor-6-ketoprostaglandin F1alpha; C049235:11-dehydro-thromboxane B2; D012643:Selenium; D015032:Zinc