Probing the structure of the dimeric KtrB membrane protein.
The KtrAB ion transporter is a complex of two proteins, KtrB and KtrA. The integral membrane protein KtrB is expected to adopt the structural architecture typified by the pore domain of potassium channels. Here we show that homo-dimerization of KtrB proteins is most likely a general property of this family of transporters. Using cysteine mutants and bifunctional cross-linkers we define regions of the Bacillus subtilis KtrB molecule that are close to the molecular 2-fold axis and to the dimer interface. Fitting of the cross-linking data to a potassium channel-like model suggests structural similarities between potassium channels and KtrB proteins in the extracellular half of the molecule and differences in the cytoplasmic regions.
Albright
Ronald A
RA
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA.
Joh
Kyu
K
Morais-Cabral
João H
JH
D000595:Amino Acid Sequence; D001407:Bacillus / Q000378:metabolism; D001412:Bacillus subtilis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003545:Cysteine / Q000737:chemistry; D003593:Cytoplasm / Q000378:metabolism; D019281:Dimerization; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D009154:Mutation; D011188:Potassium / Q000737:chemistry; D015221:Potassium Channels / Q000737:chemistry; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013296:Streptococcus pneumoniae / Q000378:metabolism
D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D003432:Cross-Linking Reagents; C113206:KtrB protein, Bacteria; D015221:Potassium Channels; D003545:Cysteine; D011188:Potassium