title: Purification and identification of adipogenesis inhibitory peptide from black soybean protein hydrolysate.
abstract: Adipogenesis inhibitory peptide was isolated and identified from black soybean (Rhynchosia volubilis Lour.) hydrolysate. An adipogenesis inhibitor was purified using consecutive methods including: ultrafiltration (MWCO; 3 and 10kDa), gel filtration chromatography (Superdex Peptide 10/300 GL column), and reverse-phase high-performance liquid chromatography (microBondapak C(18) column). Also, the adipogenesis inhibition effect of the purified peptide was measured by observation of droplet of 3T3-L1 adipocyte by Oil Red O staining in the highest active fraction in each step. The peptide was shown to inhibit the differentiation of the 3T3-L1 pre-adipocyte, which was confirmed by morphological study. The adipogenesis inhibitory peptide was purified 71.43-fold from black soybean hydrolysate throughout a five-step purification procedure. The adipogenesis inhibitor was identified to be a tripeptide, Ile-Gln-Asn, having an IC(50) value of 0.014 mg protein/ml. Furthermore, the synthetic tripeptide (Ile-Gln-Asn) exhibited the similar adipogenesis effects to the purified peptide. Thus, these results showed the potential anti-obesity effect of the purified peptide through control of adiposity.
authors.affiliation: Department of Food and Nutrition, Hanyang University, Seoul 133-791, Republic of Korea.
authors.forename: Hyun Jeong
authors.initials: HJ
authors.lastname: Kim
authors.forename: In Young
authors.initials: IY
authors.lastname: Bae
authors.forename: Chang-Won
authors.initials: CW
authors.lastname: Ahn
authors.forename: Suyong
authors.initials: S
authors.lastname: Lee
authors.forename: Hyeon Gyu
authors.initials: HG
authors.lastname: Lee
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects*; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D006868:Hydrolysis; D051379:Mice; D009842:Oligopeptides / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D030262:Soybean Proteins / Q000378:metabolism*
chemical_list: D009842:Oligopeptides; D030262:Soybean Proteins