Galanin plays a role in the conditioning lesion effect in sensory neurons.
Sensory neurons show enhanced neurite outgrowth in vivo and in vitro following a conditioning lesion. Previous studies have shown that these effects are dependent on two members of the gp130 family of cytokines, leukemia inhibitory factor and interleukin-6. Here, we asked whether galanin, a neuropeptide induced by these cytokines, plays a role in the conditioning lesion response. Following a conditioning lesion, neurite outgrowth in culture was reduced in sensory neurons from galanin -/- mice compared with those from wild type controls. In neurons from wild type mice, the length of the longest neurite was increased 2.4-fold after a conditioning lesion, compared with 1.8-fold in neurons from knockout animals. The results indicate that the induction of galanin plays an important role in triggering the conditioning lesion response.
Sachs
Hilary H
HH
Department of Neurosciences, Case Western Reserve University, Cleveland, Ohio, USA.
Wynick
David
D
Zigmond
Richard E
RE
D000818:Animals; D019771:Axotomy; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D049108:Cell Enlargement / Q000187:drug effects; D048429:Cell Size; D002478:Cells, Cultured; D019004:Galanin / Q000235:genetics / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000276:immunology / Q000378:metabolism; D053605:Leukemia Inhibitory Factor / Q000276:immunology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D016501:Neurites / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D009475:Neurons, Afferent / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D020426:Sciatic Neuropathy / Q000378:metabolism / Q000503:physiopathology
D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D019004:Galanin