title: [Study of genetic diversity of nontypeable Haemophilus influenzae strains isolated from healthy children and patients with infection symptoms].
abstract: In the study the usefulness of genotyping methods for genetic variability examinations of non-typeable H. influenzae strains circulating in population as well as level the variability of NTHi strains isolated from healthy children and from symptomatic infection cases have been evaluated. Among genotyping methods evaluated, AFLP method of the MfeI/BglII set has been found most useful to study level of genetic variability of NTHi strains population. It has been shown that NTHi strains colonizing nasopharyngeal of healthy children present higher polymorphism level than strains isolated from patient with clinical symptoms of NTHi infection.
authors.affiliation: Zakład Badania Surowic i Szczepionek PZH w Warszawie.
authors.forename: Ewa
authors.initials: E
authors.lastname: Augustynowicz
authors.forename: Leszek
authors.initials: L
authors.lastname: Szenborn
authors.forename: Alfred
authors.initials: A
authors.lastname: Samet
authors.forename: Anna
authors.initials: A
authors.lastname: Sledzińska
authors.forename: Anna
authors.initials: A
authors.lastname: Gzyl
authors.forename: Aneta
authors.initials: A
authors.lastname: Kielan
authors.forename: Janusz
authors.initials: J
authors.lastname: Slusarczyk
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods; D002353:Carrier State / Q000145:classification* / Q000382:microbiology; D002675:Child, Preschool; D015331:Cohort Studies; D006192:Haemophilus Infections / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000145:classification / Q000235:genetics*; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*