Determination of selected antioxidants in Melissae herba by isotachophoresis and capillary zone electrophoresis in the column-coupling configuration.
A method utilising isotachophoresis and capillary zone electrophoresis in the column coupling configuration with UV detection at 320 nm was developed for separation and determination of five phenolic acids (rosmarinic, p-coumaric, ferulic, caffeic and chlorogenic) and flavonoid quercitrin in a methanolic extract of Melissae herba. The proposed method has been validated with correlation coefficients from 0.9842 to 0.9988, RSD values between 0.39% and 0.83% for migration times and between 0.40% and 2.05% for peak areas.
Safra
Jirí
J
Department of Analytical Chemistry, Charles University in Prague, Faculty of Pharmacy, Hradec Králové, Czech Republic. jiri.safra@faf.cuni.cz
Pospísilová
Marie
M
Honegr
Jan
J
Spilková
Jirina
J
D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D005419:Flavonoids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D062385:Hydroxybenzoates / Q000032:analysis; D031334:Melissa / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods; D013056:Spectrophotometry, Ultraviolet
D000975:Antioxidants; D005419:Flavonoids; D062385:Hydroxybenzoates; D010936:Plant Extracts; C017616:phenolic acid; D000432:Methanol