title: Analysis on DNA methylation of various tissues in chicken.
abstract: DNA methylation is known to be associated with the transcriptional silencing in eukaryotes. In this study, the genome-wide DNA methylation patterns of CCGG sites were detected with fluorescent-labeled methylation sensitive amplified polymorphism (F-MSAP) in three chicken strains (White Leghorn, White Plymouth Rock, and their F(1) hybrids). The average incidence of DNA methylation was approximately 29.4% in muscle, 27.2% in liver, 27.2% in heart, and 26.1% in kidney, respectively. Also, the significant differences of the DNA methylation levels were found among the different tissues (P < 0.05) or strains (P < 0.05), implying that such differences may be related to gene expression during tissue differentiation, growth, and development though the relation between methylation and gene expression is currently hard to assess.
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Dongxiao
authors.initials: D
authors.lastname: Sun
authors.forename: Yachun
authors.initials: Y
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Yu
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D018899:CpG Islands; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic
chemical_list: D004247:DNA