title: Impact of ageing on lymphatic cerebrospinal fluid absorption in the rat.
abstract: Several parameters associated with the cerebrospinal fluid (CSF) system show a change in the later stages of life, including elevated CSF outflow resistance. The latter implies a CSF absorption deficit. As a significant portion of CSF absorption occurs into extracranial lymphatic vessels located in the olfactory turbinates, the purpose of this study was to determine whether any age-related impediments to CSF absorption existed at this location. In previous studies, we observed rapid movement of the CSF tracer into the olfactory turbinates in young rats (peaking 30 min after injection), with the concentration of the tracer being much higher in the turbinates than in any other tissue measured. In the study reported here, (125)I-human serum albumin was injected into the lateral ventricles of 3-, 6-, 12- and 19-month-old Fisher 344 rats. The animals were sacrificed at various times after injection of the radioactive tracer, and appropriate tissue samples were extracted. At 30 min post injection, the average tracer values expressed as per cent injected/g tissue were 6.68 +/- 0.42 (n = 9, 3 months), 4.78 +/- 0.67 (n = 9, 6 months), 2.49 +/- 0.31 (n = 9, 12 months) and 2.42 +/- 0.72 (n = 9, 19 months). We conclude that lymphatic CSF transport declines significantly with age. In concert with the known drop in CSF formation, the reduction in lymphatic CSF absorption may contribute to a decrease in CSF turnover in the elderly.
authors.affiliation: Neuroscience Program, Department of Laboratory Medicine and Pathobiology, Sunnybrook Health Sciences Centre, University of Toronto, Toronto, Ontario, Canada.
authors.forename: G
authors.initials: G
authors.lastname: Nagra
authors.forename: M G
authors.initials: MG
authors.lastname: Johnston
mesh_terms: D000042:Absorption; D000375:Aging / Q000502:physiology*; D000818:Animals; D001692:Biological Transport; D002555:Cerebrospinal Fluid / Q000378:metabolism*; D006801:Humans; D042601:Lymphatic Vessels / Q000378:metabolism*; D008297:Male; D009831:Olfactory Mucosa / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D012711:Serum Albumin, Radio-Iodinated / Q000378:metabolism; D014420:Turbinates / Q000378:metabolism
chemical_list: D012711:Serum Albumin, Radio-Iodinated