title: Folate fortification of rice by metabolic engineering.
abstract: Rice, the world's major staple crop, is a poor source of essential micronutrients, including folates (vitamin B9). We report folate biofortification of rice seeds achieved by overexpressing two Arabidopsis thaliana genes of the pterin and para-aminobenzoate branches of the folate biosynthetic pathway from a single locus. We obtained a maximal enhancement as high as 100 times above wild type, with 100 g of polished raw grains containing up to four times the adult daily folate requirement.
authors.affiliation: Unit Plant Hormone Signalling and Bio-imaging, Department of Molecular Genetics, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Sergei
authors.initials: S
authors.lastname: Storozhenko
authors.forename: Veerle
authors.initials: V
authors.lastname: De Brouwer
authors.forename: Maarten
authors.initials: M
authors.lastname: Volckaert
authors.forename: Oscar
authors.initials: O
authors.lastname: Navarrete
authors.forename: Dieter
authors.initials: D
authors.lastname: Blancquaert
authors.forename: Guo-Fang
authors.initials: GF
authors.lastname: Zhang
authors.forename: Willy
authors.initials: W
authors.lastname: Lambert
authors.forename: Dominique
authors.initials: D
authors.lastname: Van Der Straeten
mesh_terms: D010129:4-Aminobenzoic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics; D005492:Folic Acid / Q000096:biosynthesis* / Q000235:genetics*; D005527:Food, Fortified; D017343:Genes, Plant; D005818:Genetic Engineering; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011622:Pterins / Q000378:metabolism
chemical_list: D011622:Pterins; D005492:Folic Acid; D010129:4-Aminobenzoic Acid