title: Preparation of rapamycin-loaded chitosan/PLA nanoparticles for immunosuppression in corneal transplantation.
abstract: Rapamycin-loaded chitosan/polylactic acid nanoparticles with size of about 300 nm in diameter were prepared through nanoprecipitation method using cholesterol-modified chitosan as a stabilizer. The surface coating of chitosan, which was demonstrated by zeta potential measurement, endowed the nanoparticles good retention ability at the procorneal area, facilitating the sustained release of rapamycin on the corneal. The immunosuppression in corneal transplantation of the nanoparticles was investigated using rabbit as animal model, the median survival time of the corneal allografts treated with nanoparticles was 27.2+/-1.03 days and 50% grafts still remained surviving by the end of the observation, while the group treated with 0.5% rapamycin suspension was 23.7+/-3.20 days. The median survival time of drug-free nanoparticles group and untreated groups were 10.9+/-1.45 and 10.6+/-1.26 days, respectively. The results demonstrated the excellent immunosuppression of rapamycin-loaded chitosan/polylactic acid nanoparticles in corneal transplantation.
authors.affiliation: School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China. xbyuan@tju.edu.cn
authors.forename: Xu-Bo
authors.initials: XB
authors.lastname: Yuan
authors.forename: Yan-Bo
authors.initials: YB
authors.lastname: Yuan
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: En-Jiang
authors.initials: EJ
authors.lastname: Tian
authors.forename: Hui-Ming
authors.initials: HM
authors.lastname: Shun
authors.forename: Ding-Hai
authors.initials: DH
authors.lastname: Huang
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Yuan
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Sheng
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002626:Chemistry, Pharmaceutical; D048271:Chitosan; D002784:Cholesterol; D016039:Corneal Transplantation / Q000276:immunology*; D004337:Drug Carriers; D004563:Electrochemistry; D007166:Immunosuppressive Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D007553:Isotope Labeling; D019344:Lactic Acid; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D009883:Ophthalmic Solutions; D011091:Polyesters; D011108:Polymers; D011817:Rabbits; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D020123:Sirolimus / Q000008:administration & dosage* / Q000494:pharmacology*; D012995:Solubility; D016284:Technetium Tc 99m Pentetate
chemical_list: D004337:Drug Carriers; D007166:Immunosuppressive Agents; D009883:Ophthalmic Solutions; D011091:Polyesters; D011108:Polymers; D019275:Radiopharmaceuticals; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan; D002784:Cholesterol; D016284:Technetium Tc 99m Pentetate; D020123:Sirolimus