title: Evaluating the vaccine potential of an influenza A viral hemagglutinin and matrix double insertion DNA plasmid.
abstract: A DNA plasmid expressing both the influenza viral matrix protein (M1) and hemagglutinin (HA) (pHA/M1) as a potential vaccine candidate was investigated. Vaccination with pHA/M1 double insertion plasmids not only induced HA-specific protective antibodies, but also elicited HA and M1-specific CD8 T cell responses. Mice immunized with pHA/M1 dual expressing plasmid showed enhanced HA inhibition titer and increased CD69(+) CD8alpha(+) T cell response compared to groups that received either the vector or a mixture of both pHA and pM1 (pHA+pM1). Furthermore, pHA/M1 immunization resulted in improved protection against both homologous and heterologous challenges.
authors.affiliation: Laboratory of Pediatric and Respiratory Virus Diseases, Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, United States. Hang.Xie@fda.hhs.gov
authors.forename: Hang
authors.initials: H
authors.lastname: Xie
authors.forename: Teresa
authors.initials: T
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Huang
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Ye
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics* / Q000276:immunology; D007114:Immunization; D009980:Influenza A virus / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D019444:Vaccines, DNA / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; C488936:M1 protein, Influenza A virus; D019444:Vaccines, DNA; D014763:Viral Matrix Proteins