[Study on the association between estrogen receptor-alpha gene polymorphisms and pubertal skeletal developmental status in China].
OBJECTIVE
To investigate the association between estrogen receptor-alpha (ER-alpha) gene polymorphisms, pubertal bone mineral density (BMD) and bone turnover markers, to better understand the frequencies of ER-a genotypes.


METHODS
The ER-alpha Pvu II and Xba I genotypes were determined by PCR-RFLP in 158 healthy adolescent girls (12-14 years old) while BMD was measured by dual-energy X-ray absorptiometry (DEXA). The bone turnover markers (BAP, BGP, TRACP) and calcium adjust hormone (25-OH-VitD, E2) were detected by ELISA. The relationship among BMD, bone turnover markers, and polymorphisms of ER-a were examined.


RESULTS
(1) Although the bone turnover markers had a trend in ER-alpha Pvu II genotypes as pp>PP>Pp but no significant difference was found (P > 0.05). There were no significant differences found among Xba I genotypes and between Px haplotype and non-Px haplotype. (2) No association was noticed between ER-a genotype and BMD at any site of the forearm and the whole body. Compared with non-Px haplotype, the Px haplotype had lower BMD, but there was no significant difference noticed (P > 0.05).


CONCLUSIONS
ER-alpha gene Pvu II and Xba I polymorphism seemed not potentially influence on BMD and bone turnover markers in the pubertal girls in Beijing, suggesting that it might not be the major genetic factor regarding this population.
Yang
Li-chen
LC
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Piao
Jian-hua
JH
Dong
Jie
J
Huang
Zhen-wu
ZW
Zhang
Qian
Q
Ma
Guan-Sheng
GS
D015502:Absorptiometry, Photon; D000135:Acid Phosphatase / Q000097:blood; D000293:Adolescent; D000469:Alkaline Phosphatase / Q000097:blood; D015519:Bone Density / Q000235:genetics / Q000502:physiology; D001846:Bone Development / Q000235:genetics*; D002648:Child; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay; D047628:Estrogen Receptor alpha / Q000235:genetics; D005260:Female; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D015675:Osteocalcin / Q000097:blood; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D011627:Puberty / Q000097:blood / Q000235:genetics*; D014807:Vitamin D / Q000097:blood
D047628:Estrogen Receptor alpha; D015675:Osteocalcin; D014807:Vitamin D; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase