title: Quantitative expression analysis in peripheral blood of patients with chronic myeloid leukaemia: correlation between HMGA2 expression and white blood cell count.
abstract: The architectural transcription factor HMGA2 is highly expressed during embryogenesis but scarcely detectable in non-dividing adult cells. Previously, HMGA2 re-expression was detected in blood from CML patients by conventional RT-PCR, while blood samples from healthy volunteers were HMGA2 negative. Using the sensitive method of real-time quantitative RT-PCR, herein HMGA2 expression was detectable not only in peripheral blood from leukaemia patients but also in blood from healthy donors. Statistical analysis revealed a highly significant correlation between white blood cell count and HMGA2 transcript levels. The results indicate that up-regulation of HMGA2 expression is correlated to the undifferentiated phenotype of leukaemic cells accumulating during progression of chronic phase to blast crisis.
authors.affiliation: Centre for Human Genetics, University of Bremen, Bremen, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Meyer
authors.forename: D
authors.initials: D
authors.lastname: Krisponeit
authors.forename: C
authors.initials: C
authors.lastname: Junghanss
authors.forename: H
authors.initials: H
authors.lastname: Murua Escobar
authors.forename: J
authors.initials: J
authors.lastname: Bullerdiek
mesh_terms: D000328:Adult; D000368:Aged; D001752:Blast Crisis; D018450:Disease Progression; D015973:Gene Expression Regulation, Leukemic; D025743:HMGA2 Protein / Q000096:biosynthesis*; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000378:metabolism; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood* / Q000378:metabolism*; D007958:Leukocyte Count; D008875:Middle Aged; D015233:Models, Statistical; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016896:Treatment Outcome; D015854:Up-Regulation
chemical_list: D025743:HMGA2 Protein