Role of Ace2 (Activator of Cellulases 2) within the xyn2 transcriptosome of Hypocrea jecorina.
Ace2 (Activator of Cellulases 2)-encoding gene was deleted from and retransformed in the H. jecorinaQM9414 genome. Comparison of xylanase activity and xyn2 transcription of the corresponding strains after cultivation on inducing compounds (xylan, xylobiose) revealed a faster initial inducibility in the Deltaace2-strain, but final levels of xyn2 transcript and xylanase activity of the parental strain could not be reached. This suggests a role for Ace2 in the regulation of xyn2 induction mechanisms, moreover Ace2 is responsible for the basal level of xyn2 transcription. Furthermore, a palindrome in the xyn2 promoter consisting of a GGGTAA- and a CCAGCC-element was identified. Both Xyr1 and Ace2 are able to bind the complete motif, the latter also only to one part of it. Phosphorylation as well as dimerization are prerequisites for binding of Ace2 to the xyn2 promoter. Finally, the impact of Ace2 on xyr1 transcription could be demonstrated under inducing conditions.
Stricker
Astrid R
AR
Gene Technology, Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Wien, Getreidemarkt 9/166/5/2, A-1060 Wien, Austria.
Trefflinger
Peter
P
Aro
Nina
N
Penttilä
Merja
M
Mach
Robert L
RL
D001665:Binding Sites; D004271:DNA, Fungal / Q000378:metabolism; D019281:Dimerization; D004187:Disaccharides / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D005816:Genetic Complementation Test; D035901:Hypocrea / Q000201:enzymology* / Q000502:physiology*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014158:Transcription, Genetic / Q000502:physiology*; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000096:biosynthesis*
D004271:DNA, Fungal; D004187:Disaccharides; D005656:Fungal Proteins; D014990:Xylans; D014995:Xylosidases; C004173:xylobiose