title: Methotrexate reduces the levels of pentosidine and 8-hydroxy-deoxy guanosine in patients with rheumatoid arthritis.
abstract: This study was performed to investigate whether methotrexate (MTX) affects the levels of oxidative stress markers, including pentosidine one of the glycation end products (AGEs) or 8-hydroxy-deoxy guanosine (8-OHdG). These stress markers represent DNA damage; 19 rheumatoid arthritis (RA) patients underwent MTX treatment. The levels of serum total, urinary total, urinary-free pentosidine and also urinary 8-OHdG, as well as clinical parameters, including disease activity scores for 28 joints (DAS28) were measured at baseline and at 3 and 6 months after the initial treatment with MTX. After the initial treatment with MTX, serum total and urinary total pentosidine levels were reduced at 6 months, and urinary-free pentosidine levels were reduced at 3 and 6 months. Urinary 8-OHdG levels also were significantly reduced at 6 months after the initial treatment with MTX. This study demonstrated that MTX plays a role as a regulator against pentosidine formation and oxidative DNA damage in RA patients.
authors.affiliation: Department of Orthopaedic Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu, 431-3125, Japan. Tsukatonpipi@nifty.com
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Kageyama
authors.forename: Masaaki
authors.initials: M
authors.lastname: Takahashi
authors.forename: Tetsuyuki
authors.initials: T
authors.lastname: Nagafusa
authors.forename: Eiji
authors.initials: E
authors.lastname: Torikai
authors.forename: Akira
authors.initials: A
authors.lastname: Nagano
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D018501:Antirheumatic Agents / Q000494:pharmacology; D001120:Arginine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000097:blood; D001172:Arthritis, Rheumatoid / Q000188:drug therapy* / Q000378:metabolism*; D003404:Creatinine / Q000652:urine; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000652:urine; D006801:Humans; D007596:Joints / Q000473:pathology; D008239:Lysine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000097:blood; D008727:Methotrexate / Q000494:pharmacology*; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D017382:Reactive Oxygen Species; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D018501:Antirheumatic Agents; D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D001120:Arginine; D003404:Creatinine; C062187:pentosidine; D003849:Deoxyguanosine; D008239:Lysine; D010100:Oxygen; D008727:Methotrexate