title: Detection and isolation of exotic Newcastle disease virus from field-collected flies.
abstract: Flies were collected by sweep net from the vicinity of two small groups of "backyard" poultry (10-20 chickens per group) that had been identified as infected with exotic Newcastle disease virus (family Paramyxoviridae, genus avulavirus, ENDV) in Los Angeles County, CA, during the 2002-2003 END outbreak. Collected flies were subdivided into pools and homogenized in brain-heart infusion broth with antibiotics. The separated supernatant was tested for the presence of ENDV by inoculation into embryonated chicken eggs. Exotic Newcastle disease virus was isolated from pools of Phaenicia cuprina (Wiedemann), Fannia canicularis (L.), and Musca domestica L., and it was identified by hemagglutination inhibition with Newcastle disease virus antiserum. Viral concentration in positive pools was low (<1 egg infectious dose50 per fly). Isolated virus demonstrated identical monoclonal antibody binding profiles as well as 99% sequence homology in the 635-bp fusion gene sequence compared with ENDV recovered from infected commercial egg layer poultry during the 2002 outbreak.
authors.affiliation: Department of Entomology, University of California, Riverside, CA 92521, USA.
authors.forename: Seemanti
authors.initials: S
authors.lastname: Chakrabarti
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: King
authors.forename: Claudio
authors.initials: C
authors.lastname: Afonso
authors.forename: David
authors.initials: D
authors.lastname: Swayne
authors.forename: Carol J
authors.initials: CJ
authors.lastname: Cardona
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Kuney
authors.forename: Alec C
authors.initials: AC
authors.lastname: Gerry
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000378:metabolism; D002140:California; D004175:Diptera / Q000821:virology*; D006793:Houseflies / Q000821:virology; D008969:Molecular Sequence Data; D009117:Muscidae / Q000821:virology; D009521:Newcastle Disease / Q000635:transmission / Q000821:virology*; D009522:Newcastle disease virus / Q000302:isolation & purification*; D011200:Poultry / Q000821:virology*; D014760:Viral Fusion Proteins / Q000235:genetics
chemical_list: D000914:Antibodies, Viral; D014760:Viral Fusion Proteins