[Effectiveness of PCR analysis in the diagnostics of tuberculosis].
To discover DNA of mycobacterium and evaluate chemotherapy efficiency of pulmonary tuberculosis Polimerize Chain Reaction (PCR) analysis was performed. Different pathological materials of patients with pulmonary tuberculosis and those with nonspecific lung diseases had been examined. Phlegm sample had been carried out regularly by means of PCR analysis and traditional microbiological methods. PCR analysis demonstrated 94% sensibility, specificity 100%; investigation of different pathological materials demonstrated 54% sensibility of molecular - genetic method. Discontinuance of bacterium secretion at PCR analysis positively correlated with clinical rontgenologic dynamics of disappearance of tubercular process.
Aliev
K A
KA
Salimova
N A
NA
D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
D004269:DNA, Bacterial