title: Arabidopsis POT1A interacts with TERT-V(I8), an N-terminal splicing variant of telomerase.
abstract: Chromosome integrity is maintained via the actions of ribonucleoprotein complexes that can add telomeric repeats or can protect the chromosome end from being degraded. POT1 (protection of telomeres 1), a class of single-stranded-DNA-binding proteins, is a regulator of telomeric length. The Arabidopsis genome contains three POT1 homologues: POT1A, POT1B and POT1C. Using yeast two-hybrid assays to identify components of a potential POT1A complex, we retrieved three interactors: the N-terminus of the telomerase, a protein kinase and a plant-specific protein. Further analysis of the interaction of POT1 proteins with telomerase showed that this interaction is specific to POT1A, suggesting a specific role for this paralogue. The interaction is specific to the N-terminal region of the telomerase, which can be encoded by splicing variants. This interaction indicates possible mechanisms for telomerase regulation by alternative splicing and by POT1 proteins.
authors.affiliation: Department of Cellular and Developmental Biology, John Innes Centre, Norwich, NR4 7UH, UK.
authors.forename: Pascale
authors.initials: P
authors.lastname: Rossignol
authors.forename: Sarah
authors.initials: S
authors.lastname: Collier
authors.forename: Max
authors.initials: M
authors.lastname: Bush
authors.forename: Peter
authors.initials: P
authors.lastname: Shaw
authors.forename: John H
authors.initials: JH
authors.lastname: Doonan
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D020134:Catalytic Domain; D002467:Cell Nucleus / Q000378:metabolism; D032461:Chromosomes, Plant / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000089804:Shelterin Complex; D019098:Telomerase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016615:Telomere; D034501:Telomere-Binding Proteins / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D009687:Nuclear Proteins; C528256:POT1A protein, Arabidopsis; C503876:Pot1 protein, Arabidopsis; D000089804:Shelterin Complex; D034501:Telomere-Binding Proteins; D019098:Telomerase