Expression of TNF-alpha protein in omental and subcutaneous adipose tissue in obesity.
BACKGROUND
During the last several years the role of adipose tissue in contributing to obesity-associated cardiovascular and metabolic risk has gained much attention.


OBJECTIVE
To examine the expression of TNF-alpha protein in omental and subcutaneous adipose tissue in correlation with plasma PAI-1 and other clinical parameters in obesity subjects.


METHODS
Paired biopsies of omental and subcutaneous fat were collected during surgery in 32 obesity subjects. The expression of TNF-alpha protein in omental and subcutaneous fat was quantified by using Western blot method, and correlations with plasma PAI-1, homeostasis model assessment insulin resistance (HOMA-IR) and lipid were investigated.


RESULTS
TNF-alpha protein expression was higher in omental than in subcutaneous adipose tissue (P<0.01). Significant positive linear correlations were found between TNF-alpha protein in omental adipose tissue and plasma PAI-1 in obesity subjects. TNF-alpha protein in omental fat was positively associated with HOMA-IR, triglycerides and negatively with HDL-cholesterol.


CONCLUSIONS
TNF-alpha expression in omental adipose tissue could play a key role in contributing to cardiovascular risk in central obesity subjects.
Cao
Yan Li
YL
Department of Endocrinology, First Affiliated Hospital of China Medical University, No. 155, Nanjing North Street, Heping District, Shenyang, Liaoning 110001, China.
Hu
Cui Zhu
CZ
Meng
Xin
X
Wang
Di Fei
DF
Zhang
Jin
J
D000273:Adipose Tissue / Q000503:physiopathology; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000453:epidemiology; D005260:Female; D015391:Gastroplasty; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000150:complications / Q000503:physiopathology* / Q000601:surgery; D009852:Omentum; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D012867:Skin; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D001786:Blood Glucose; D017395:Plasminogen Activator Inhibitor 1; D014409:Tumor Necrosis Factor-alpha