title: Tissue factor activated thromboelastography correlates to clinical signs of bleeding in dogs.
abstract: The ability of a laboratory assay to correlate to clinical phenotype is crucial for the accurate diagnosis and monitoring of haemostasis and is therefore challenging with currently used routine haemostasis assays. Thromboelastography (TEG) is increasingly used to evaluate haemostasis in humans and may well be of value in the workup of dogs suspected of having a haemostatic disorder. This study was undertaken to evaluate prospectively how tissue factor (TF) activated TEG correlated to clinical signs of bleeding in dogs, compared to a routine coagulation profile. A prospective case-control study was performed over a 2 year period from 2004-2006. Eligible dogs were those where the primary clinician requested a coagulation profile to evaluate haemostasis. The dogs were simultaneously evaluated with a TF-activated TEG assay. Twenty-seven dogs, characterised as hypo-coagulable based on the TEG parameter G (<3.2 Kdyn/cm(2)), were included in the study as cases. Size matched control groups of TEG normo- (G=3.2K-7.2 Kdyn/cm(2)) and hyper-coagulable (G>7.2 Kdyn/cm(2)) dogs were selected retrospectively from the eligible dogs. For all dogs, clinical signs of bleeding were noted at time of analysis. There were statistically significant differences between all TEG values of hypo- and normo- and hyper-coagulable dogs. Thromboelastography correctly identified dogs with clinical signs of bleeding with a positive predictive value (PPV) of 89% and a negative predictive value (NPV) of 98% based on G alone. In comparison, the coagulation profile had a PPV between 50-81% and a NPV between 92-93% for detection of bleeding, depending on the observer. In conclusion, a TF-activated TEG G value<3.2K dyn/cm(2) correctly identified dogs with clinical signs of bleeding with very high PPV and NPV, irrespective of observer. The findings strongly suggest that TF- activated TEG may be of value in the workup of dogs suspected of having a haemostatic disorder.
authors.affiliation: The Small Animal Hospital, Department of Small Animal Clinical Sciences, Faculty of Life Sciences, University of Copenhagen, Frederiksberg DK-1870, Denmark. bwi@life.ku.dk
authors.forename: Bo
authors.initials: B
authors.lastname: Wiinberg
authors.forename: Asger L
authors.initials: AL
authors.lastname: Jensen
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Rozanski
authors.forename: Pär I
authors.initials: PI
authors.lastname: Johansson
authors.forename: Mads
authors.initials: M
authors.lastname: Kjelgaard-Hansen
authors.forename: Mikael
authors.initials: M
authors.lastname: Tranholm
authors.forename: Annemarie T
authors.initials: AT
authors.lastname: Kristensen
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000502:physiology; D001780:Blood Coagulation Tests / Q000379:methods / Q000662:veterinary; D016022:Case-Control Studies; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis*; D004285:Dogs / Q000097:blood*; D005260:Female; D020141:Hemostatic Disorders / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D006706:Homeostasis; D008297:Male; D011237:Predictive Value of Tests; D011446:Prospective Studies; D013916:Thrombelastography / Q000379:methods / Q000662:veterinary*; D013925:Thromboplastin / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D013925:Thromboplastin