title: Bioprotection of tendon repair: adjunctive use of botulinum toxin A in Achilles tendon repair in the rat.
abstract: BACKGROUND
  Tendon-repair techniques have evolved to increase the construct strength of the repair site in order to permit early active range of motion without tendon gap or rupture. The present study evaluated the hypothesis that the injection of botulinum neurotoxin type-A (BoNT-A) into the gastrocnemius muscle will reduce the active force production of that muscle below the force required to rupture the associated, repaired Achilles tendon.
  
  
  METHODS
  Seventy-nine rat Achilles tendons were surgically bisected and were repaired with use of a two-strand core suture with a running epitenon repair. After the repair, the animals were treated with unilateral intramuscular (gastrocnemius) injections of either BoNT-A (6 U/kg body weight) (thirty-seven rats) or saline solution (forty-two rats). Operatively treated ankles were fixed in the neutral position with a percutaneous pin for the first two days after surgery. Unrestricted ankle motion and weight-bearing were allowed after the second postoperative day. An assessment of gap formation or rupture at the repair site, electrophysiologic measurements of force applied to the tendon, and an assessment of the strength of the repaired tendon were performed.
  
  
  RESULTS
  Intramuscular BoNT-A injections produced a significant, reversible reduction in active muscle force (p < 0.007). Twitch and tetanus contractions decreased to approximately 25% of the values for the control side within one week, remained at <50% of the values for the control side at one month, and returned to normal levels by six months. The tetanic force capability of the muscles that had been injected with BoNT-A was fivefold to tenfold less than the force required to rupture the associated Achilles tendon for as long as four weeks after tendon repair. The spontaneous Achilles tendon rupture rate of repaired tendons in the BoNT-A group was three times lower than that in the saline solution group at one week, and the tendon rupture force was significantly higher in the BoNT-A group between one and three weeks after repair (p < 0.007). There was no significant difference in tendon rupture force between the two groups after three weeks.
  
  
  CONCLUSIONS
  Intramuscular gastrocnemius BoNT-A injections were associated with a significant reduction in force-generating potential, such that the muscle was incapable of actively producing enough force to rupture the repaired Achilles tendon in this rat model of tendon repair.
authors.affiliation: Department of Orthopaedic Surgery, Wake Forest University Health Sciences, Medical Center Boulevard, Winston-Salem, NC 27157, USA.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Ma
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
authors.forename: Beth Paterson
authors.initials: BP
authors.lastname: Smith
authors.forename: Andrew
authors.initials: A
authors.lastname: Ritting
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Smith
authors.forename: L Andrew
authors.initials: LA
authors.lastname: Koman
mesh_terms: D000125:Achilles Tendon / Q000293:injuries* / Q000503:physiopathology / Q000601:surgery*; D000818:Animals; D019274:Botulinum Toxins, Type A / Q000008:administration & dosage*; D007273:Injections, Intramuscular; D053580:Muscle Strength / Q000187:drug effects*; D018482:Muscle, Skeletal / Q000187:drug effects*; D009465:Neuromuscular Agents / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012421:Rupture / Q000503:physiopathology / Q000517:prevention & control / Q000601:surgery; D013536:Suture Techniques
chemical_list: D009465:Neuromuscular Agents; D019274:Botulinum Toxins, Type A