title: Characterization of ScORK28, a transmembrane functional protein receptor kinase predominantly expressed in ovaries from the wild potato species Solanum chacoense.
abstract: Solanum chacoense ovule receptor kinase 28 (ScORK28) was found among 30 receptor kinases from an ovule cDNA library enriched for weakly expressed mRNAs. This LRR-RLK displayed high level of tissue specificity at the RNA and protein levels and was predominantly expressed in female reproductive tissues. Protein expression analyses in planta revealed that ScORK28 was N-glycosylated and ScORK28::GFP fusion analyses showed that it was localized at the plasma membrane. Bacterial expression of ScORK28 catalytic domain followed by kinase activity assays revealed that ScORK28 is an active Mg2+-dependent protein kinase and that the juxtamembrane domain is necessary for kinase activity.
authors.affiliation: Institut de Recherche en Biologie Végétale (IRBV), Département de Sciences Biologiques, Université de Montréal, 4101 Sherbrooke Est, Montréal, QC, Canada H1X 2B2.
authors.forename: Hugo
authors.initials: H
authors.lastname: Germain
authors.forename: Josée
authors.initials: J
authors.lastname: Houde
authors.forename: Madoka
authors.initials: M
authors.lastname: Gray-Mitsumune
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Sawasaki
authors.forename: Yaeta
authors.initials: Y
authors.lastname: Endo
authors.forename: Jean
authors.initials: J
authors.lastname: Rivoal
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Matton
mesh_terms: D000595:Amino Acid Sequence; D020134:Catalytic Domain; D002462:Cell Membrane / Q000201:enzymology*; D004926:Escherichia coli / Q000235:genetics; D008274:Magnesium / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D011494:Protein Kinases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D032322:Solanum / Q000201:enzymology* / Q000235:genetics
chemical_list: D008565:Membrane Proteins; D010940:Plant Proteins; D011494:Protein Kinases; D008274:Magnesium