[Study on correlation between HLA-A, B, DR alleles and Duchenne muscular dystrophy].
OBJECTIVE
To analyze the polymorphism of HLA-A, B and DR alleles of Duchenne muscular dystrophy (DMD) patients in Han nationality of South China and to discuss the role of immune and genetic factors in the pathogenesis of DMD and muscular fiber necrosis.


METHODS
Polymerase chain reaction-reverse sequence specific oligonucleotide (PCR-RSSO) and National Marrow Donor Program (NMDP) were used to analyze the polymorphism of HLA-A,B and DR alleles of 113 DMD patients and 406 normal controls in Han nationality of South China.


RESULTS
The frequencies of HLA-A24, A30 alleles in DMD group were 11.25% and 5.46% respectively, indicating notable difference (P=0.001, < 0.01) from 22.16% and 0.87% of control group; the frequencies of HLA-B13, B15, B61 and B62 alleles in DMD group were 12.26%, 16.92%, 0.44% and 0.44%, indicating a notable difference (P=0.016, < 0.01, 0.001) from 6.76%, 1.49%, 4.79% and 5.05% of control group; the frequencies of HLA-DR04, DR07, DR12 alleles in DMD group were 17.45%, 6.40% and 19.62%, indicating a notable difference (P=0.018, < 0.01, 0.012) from 10.67%, 2.24% and 11.92% of control group.


CONCLUSIONS
There are significant differences in the HLA gene frequencies between DMD patients and normal controls. These results suggest that HLA genotype relates to the muscular necrosis and the pathogenesis of DMD.
Chen
Wei
W
Department of Cerebral Angiopathy, Shenzhen Nanshan Hospital, Guang dong Medical College, Shenzhen, Guangdong, 518052 PR China.
Xiao
Lulu
L
Zhang
Cheng
C
Wu
Hong-ling
HL
D000293:Adolescent; D000483:Alleles; D002648:Child; D002675:Child, Preschool; D002681:China / Q000208:ethnology; D005006:Ethnicity / Q000235:genetics; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D006801:Humans; D020388:Muscular Dystrophy, Duchenne / Q000235:genetics* / Q000276:immunology; D011110:Polymorphism, Genetic
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006684:HLA-DR Antigens