Measurement of electroosmotic flow in capillary and microchip electrophoresis.
Microfluidics is the science and technology of systems that process or manipulate small amounts of fluids, using channels with dimensions of tens of micrometers. Electroosmotic flow (EOF) is an important characteristic of fluids in microchannels. In this paper, EOF generation, effects on separation and definition of EOF are introduced. And EOF measurement methods on capillary electrophoresis (CE) and microchip CE are systematically reviewed based on detection principle, hallmarks of EOF measurement methods are presented, the devices and signals are also schematically described. This paper offers researchers a guidance to obtain an estimate of EOF mobility in capillary and microchip electrophoresis.
Wang
Wei
W
Department of Chemistry, Key Lab of Analytical Chemistry for Life Science, Nanjing University, Nanjing 210093, China.
Zhou
Fang
F
Zhao
Liang
L
Zhang
Jian-Rong
JR
Zhu
Jun-Jie
JJ
D000465:Algorithms; D002021:Buffers; D003217:Conductometry; D004129:Dimethylpolysiloxanes; D004563:Electrochemistry / Q000379:methods; D053841:Electroosmosis / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods*; D046208:Microchip Analytical Procedures; D044085:Microfluidics / Q000379:methods; D009995:Osmosis
D002021:Buffers; D004129:Dimethylpolysiloxanes