title: Oxygen and temperature-dependent structural and redox changes in a novel cytochrome c(4) from the purple sulfur photosynthetic bacterium Thiocapsa roseopersicina.
abstract: A novel cytochrome c(4), the first of this type in purple phototrophic bacteria has been discovered in Thiocapsa roseopersicina. The fact that cytochrome c(4) has been found in an anaerobic organism puts in question the up hereto suggested role of cytochromes c(4) in the aerobic respiratory metabolism. The structure of cytochrome c(4) was studied under both aerobic and anaerobic conditions, using differential scanning calorimetry and a combination of redox potentiostatic measurements with CD and UV-Vis absorption techniques. Cytochrome c(4) maintained its functional capability at high temperature (60 degrees C) if it was kept under anaerobic conditions. With increasing temperature under aerobic conditions, however, there are dramatic conformational changes in the protein and coordination changes on the iron side. Presumably oxygen binds to the iron at the position left vacant by the methionine and facilitates conformational changes with low reversibility.
authors.affiliation: Institute of Biophysics, Temesvári krt. 62, H-6726 Szeged, Hungary.
authors.forename: Rui M M
authors.initials: RM
authors.lastname: Branca
authors.forename: Gabriella
authors.initials: G
authors.lastname: Bodó
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Várkonyi
authors.forename: Mónika
authors.initials: M
authors.lastname: Debreczeny
authors.forename: Judit
authors.initials: J
authors.lastname: Osz
authors.forename: Csaba
authors.initials: C
authors.lastname: Bagyinka
mesh_terms: D003198:Computer Simulation; D003574:Cytochrome c Group / Q000737:chemistry* / Q000648:ultrastructure*; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D011487:Protein Conformation; D013696:Temperature; D020616:Thiocapsa roseopersicina / Q000201:enzymology*
chemical_list: D003574:Cytochrome c Group; C034186:cytochrome C4; D010100:Oxygen