Proteomic profiling of a parasitic wasp exposed to constant and fluctuating cold exposure.
When insects are exposed to fluctuating thermal regimes (FTRs) (i.e., cold exposure alternating with periodic short pulses to high temperature), in contrast to constant low temperature (CLT), mortality due to accumulation of chill injuries is markedly reduced. To investigate the physiological processes behind the positive impact of FTR, based on a holistic approach, two-dimensional electrophoresis (2-DE) analysis were performed with the parasitic wasp Aphidius colemani. Parasitoid proteomes revealed 369 well-distinguishable protein spots, where the overall response to cold exposure was clearly specific to treatments (CLT versus FTR). The reduced mortality under FTR was associated with up-regulation of several proteins playing key roles in energy metabolism (glycolysis, TCA cycle, synthesis and conversion of ATP), protein chaperoning (Hsp70/Hsp90), and protein degradation (proteasome). Our results also support the idea that cytoskeleton components, particularly actin arrangement, could play a role in the higher survival rates of insects under FTR.
Colinet
Hervé
H
Unité d'Ecologie et de Biogéographie, Centre de Recherche sur la Biodiversité, Université Catholique de Louvain, Croix du Sud 4-5, B-1348 Louvain-la-Neuve, Belgium. colinet@ecol.ucl.ac.be
Nguyen
Thi Thuy An
TT
Cloutier
Conrad
C
Michaud
Dominique
D
Hance
Thierry
T
D000064:Acclimatization / Q000235:genetics; D000818:Animals; D003080:Cold Temperature; D015180:Electrophoresis, Gel, Two-Dimensional; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D014863:Wasps / Q000378:metabolism* / Q000502:physiology
D019476:Insect Proteins