Absorption of dietary licorice isoflavan glabridin to blood circulation in rats.
Bioavailability of glabridin was elucidated to show that this compound is one of the active components in the traditional medicine licorice. Using a model of intestinal absorption, Caco-2 cell monolayer, incorporation of glabridin was examined. Glabridin was easily incorporated into the cells and released to the basolateral side at a permeability coefficient of 1.70+/-0.16 cm/s x 10(5). The released glabridin was the aglycone form and not a conjugated form. Then, 10 mg (30 micromol)/kg body weight of standard chemical glabridin and licorice flavonoid oil (LFO) containing 10 mg/kg body weight of glabridin were administered orally to rats, and the blood concentrations of glabridin was determined. Glabridin showed a maximum concentration 1 h after the dose, of 87 nmol/L for standard glabridin and 145 nmol/L for LFO glabridin, and decreased gradually over 24 h after the dose. The level of incorporation into the liver was about 0.43% of the dosed amount 2 h after the dose. These detected glabridins were in the aglycone form and not conjugated forms. The bioavailability was calculated to be AUC(inf) of 0.825 and 1.30 microM.h and elimination T(1/2 )of 8.2 and 8.5 h for standard glabridin and LFO, respectively. Adipocytokine levels were determined in the rats. The secreted amount of monocyte chemoattractant protein-1 was significantly lower in the glabridin group compared to control vehicle group. Thus, dietary glabridin was at least partly incorporated into the body in an unchanged form, though most dietary flavonoids are converted to non-active conjugate forms during intestinal absorption.
Ito
Chinatsu
C
Laboratory of Food and Nutritional Chemistry, Graduate School of Agriculture, Kobe University, Kobe, Japan.
Oi
Naomi
N
Hashimoto
Takashi
T
Nakabayashi
Hideo
H
Aoki
Fumiki
F
Tominaga
Yuji
Y
Yokota
Shinichi
S
Hosoe
Kazunori
K
Kanazawa
Kazuki
K
D052242:Adiponectin / Q000097:blood / Q000378:metabolism; D000818:Animals; D001682:Biological Availability; D018938:Caco-2 Cells; D002470:Cell Survival / Q000502:physiology; D018932:Chemokine CCL2 / Q000097:blood; D006035:Glycyrrhiza / Q000737:chemistry; D006801:Humans; D007328:Insulin / Q000097:blood / Q000378:metabolism; D000078790:Insulin Secretion; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D007529:Isoflavones; D020738:Leptin / Q000097:blood / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D010636:Phenols / Q000097:blood* / Q000493:pharmacokinetics*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism
D052242:Adiponectin; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D007328:Insulin; D007529:Isoflavones; D020738:Leptin; D010636:Phenols; D014409:Tumor Necrosis Factor-alpha; C107601:glabridin