title: In vitro comparison of the biocompatibility of mineral trioxide aggregate, 4META/MMA-TBB resin, and intermediate restorative material as root-end-filling materials.
abstract: This study compared the effects of three root-end-filling materials: mineral trioxide aggregate (MTA), 4-META/MMA-TBB resin (Super-bond), and intermediate restorative material (IRM) on osteoblast adhesion, proliferation, and matrix formation. MC3T3-E1 cells were inoculated on set material specimens, and the number of cells adhered to each material was assessed at 6 hours and at 3 days of incubation by using Hoechst 33258 staining under a fluorescence microscope. Furthermore, on day 21 of culture, areas in contact with each material surface were observed at light and electron microscopic levels. Cultured cells that adhered to MTA and Super-bond proliferated significantly between 6 hours and 3 days of culture. In addition, on day 21, they produced a thick matrix layer directly on the material surfaces. In contrast, the number of cells that adhered to IRM significantly decreased with time. These cells appeared round in shape, and no matrix formation was found. These results suggest that MTA and Super-bond have good biocompatibility and allow hard-tissue forming cells to create a matrix layer, which might enhance apical tissue regeneration.
authors.affiliation: Department of Endodontology and Operative Dentistry, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. yoshimin@dent.kyushu-u.ac.jp
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Yoshimine
authors.forename: Makiko
authors.initials: M
authors.lastname: Ono
authors.forename: Akifumi
authors.initials: A
authors.lastname: Akamine
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D001896:Boron Compounds / Q000494:pharmacology*; D017610:Calcium Compounds / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D004338:Drug Combinations; D008689:Methacrylates / Q000494:pharmacology*; D008768:Methylmethacrylates / Q000494:pharmacology*; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects*; D010087:Oxides / Q000494:pharmacology*; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology*
chemical_list: D017607:Aluminum Compounds; D001896:Boron Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D008689:Methacrylates; D008768:Methylmethacrylates; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D015035:Zinc Oxide-Eugenol Cement; C086631:mineral trioxide aggregate; C031667:IRM cement; C049495:Super-bond