Yeast expression platforms.
Yeasts provide attractive expression platforms. They combine ease of genetic manipulations and the option for a simple fermentation design of a microbial organism with the capabilities of an eukaryotic organism to secrete and to modify a protein according to a general eukaryotic scheme. For platform applications, a range of yeast species has been developed during the last decades. We present in the following review a selection of established and newly defined expression systems. The review is concluded by the description of a wide-range vector system that allows the assessment of the selected organisms in parallel for criteria like secretion or appropriate processing and modification in a given case.
Böer
Erik
E
Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, 06466, Gatersleben, Germany.
Steinborn
Gerhard
G
Kunze
Gotthard
G
Gellissen
Gerd
G
D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005818:Genetic Engineering; D005822:Genetic Vectors; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism*
D005656:Fungal Proteins