Effects of short-term exercise on adiponectin and adiponectin receptor levels in rats.
OBJECTIVE
Adiponectin reportedly reduces insulin resistance. Exercise has also been shown to lessen insulin resistance, although it is not well known whether exercise increases levels of adiponectin and/or its receptors nor whether it effects are dependent on exercise intensity and/or period. We previously reported that blood adiponectin levels increased by 150% in animals that exercised at a rate of 30 m/min for 60 minutes, 2 days per week, and adiponectin receptor 1 (AdipoR1) mRNA levels in muscle increased up to 4 times in response to exercise at a rate of 25 m/min for 30 min, 5 days per week for 12 weeks.


METHODS
In light of this information, we examined the effects of short-term exercise on adiponectin, and adiponectin receptor levels in rats, using ELISA and real-time PCR.


RESULTS
Our data showed that adiponectin mRNA levels in adipose tissue increased by 280% in rats exercised at a rate of 30 m/min for 60 minutes for 2 weeks and correlated with the exercise time periods. No effects of short-term exercise on adiponectin receptor 1 mRNA in muscle were observed.


CONCLUSIONS
Thus, long-term exercise may be required to regulate adiponectin receptor 1 mRNA expression in muscle and adiponectin mRNA expression in adipose tissue.
Zeng
Qin
Q
Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
Fu
Ling
L
Takekoshi
Kazuhiro
K
Kawakami
Yasushi
Y
Isobe
Kazumasa
K
D052242:Adiponectin / Q000096:biosynthesis*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D004837:Epinephrine / Q000652:urine; D015870:Gene Expression; D008055:Lipids / Q000097:blood; D010805:Physical Conditioning, Animal / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D054419:Receptors, Adiponectin / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013995:Time
D052242:Adiponectin; D001786:Blood Glucose; D008055:Lipids; D012333:RNA, Messenger; D054419:Receptors, Adiponectin; D004837:Epinephrine