title: [Identification of a novel HLA allele B 4446 by sequencing-based typing].
abstract: OBJECTIVE
  To identify a novel HLA allele in Chinese population.
  
  
  METHODS
  A new HLA-B allele was initially detected by an usual PCR-SSP and PCR-SSOP in routine typing HLA allele. Sequence-based typing (SBT) was used to identify and analysis the difference between the new allele and HLA-B 4409 allele.
  
  
  RESULTS
  The HLA-B exon 3 nucleotide sequence of the novel allele was different from all other known alleles. The allele had 3 nucleotides replaced of the closest matching B 4409 allele at nt538(G>C), nt539(A>T) and nt540 (C>G) in exon 3, resulting in an amino acid change from D(GAC) to L(CTG) at codon 180.
  
  
  CONCLUSIONS
  A novel HLA allele was confirmed by the SBT and it was officially designated as HLA-B 4446 by WHO Nomenclature Committee for Factors of the HLA System in September,2005.
authors.affiliation: Liaoning Blood Center, Shenyang, Liaoning, 110044 P. R. China. 123@syxz.com.
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Li
authors.forename: Zhe
authors.initials: Z
authors.lastname: Qu
authors.forename: Jing-bo
authors.initials: JB
authors.lastname: Gao
authors.forename: Xu-ying
authors.initials: XY
authors.lastname: Huang
authors.forename: Xian-zhi
authors.initials: XZ
authors.lastname: Liu
mesh_terms: D000483:Alleles; D001483:Base Sequence; D015235:HLA-B Antigens / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D015235:HLA-B Antigens