title: Growth rate of a non-fermentative Escherichia coli strain is influenced by NAD+ regeneration.
abstract: By complementing a non-fermentative Escherichia coli (ldhA (-) pflB (-)) strain with the recombinant Zymomonas mobilis ethanol pathway (pdc, adhB), we evaluated the effect of different levels of enzymatic activity on growth rate demonstrating that there is a direct relationship between anaerobic growth rate and the total specific activity of pyruvate decarboxylase, which is the limiting enzyme of this specific fermentative NAD(+) regenerating pathway. This relationship was proved to be useful to establish a selection strategy based on growth rate for the analysis of lctE libraries, which encode lactate dehydrogenase from Bacillus subtilis.
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, Morelos, 62250, Mexico, joel@ibt.unam.mx.
authors.forename: Consuelo
authors.initials: C
authors.lastname: Vázquez-Limón
authors.forename: Joel
authors.initials: J
authors.lastname: Vega-Badillo
authors.forename: Alfredo
authors.initials: A
authors.lastname: Martínez
authors.forename: Gabriela
authors.initials: G
authors.lastname: Espinosa-Molina
authors.forename: Guillermo
authors.initials: G
authors.lastname: Gosset
authors.forename: Xavier
authors.initials: X
authors.lastname: Soberón
authors.forename: Agustín
authors.initials: A
authors.lastname: López-Munguía
authors.forename: Joel
authors.initials: J
authors.lastname: Osuna
mesh_terms: D000693:Anaerobiosis; D015169:Colony Count, Microbial; D019020:Directed Molecular Evolution; D004926:Escherichia coli / Q000166:cytology / Q000254:growth & development*; D005285:Fermentation; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D016296:Mutagenesis; D050505:Mutant Proteins / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D011767:Pyruvate Decarboxylase / Q000378:metabolism; D017355:Zymomonas / Q000201:enzymology
chemical_list: D050505:Mutant Proteins; D009243:NAD; D007770:L-Lactate Dehydrogenase; D011767:Pyruvate Decarboxylase