Apoptosis-associated changes in the glycerophospholipid composition of hematopoietic progenitor cells monitored by 31P NMR spectroscopy and MALDI-TOF mass spectrometry.
Apoptosis, or programmed cell death, plays an important role in development and in tissue homeostasis and is assumed to be accompanied by changes in the composition of cellular glycerophospholipids (GPL). We have applied a combination of 31P nuclear magnetic resonance spectroscopy and matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) to the analysis of organic extracts of hematopoietic progenitor cells undergoing the physiologically relevant process of apoptosis following growth factor withdrawal. The combined application of these methods enables the quantitative analysis of all glycerophospholipid classes and reveals changes in the acyl chain compositions from crude cell extracts. Using these techniques, an increase in the ratio of ether-linked glycerophospholipids to diacyl-glycerophospholipids during apoptosis was observed. The relative decrease in the membrane diacyl-phosphatidylcholine (PC) levels was found to correlate with increased concentrations of the corresponding lysophosphatidylcholine (LPC) present in the medium.
Fuchs
Beate
B
University of Leipzig, Medical Faculty, Institute of Medical Physics and Biophysics, Härtelstr. 16-18, D-04107 Leipzig, Germany. Beate.Fuchs@medizin.uni-leipzig.de
Schiller
Jürgen
J
Cross
Michael A
MA
D017209:Apoptosis; D001671:Biochemistry / Q000379:methods*; D004983:Ethanolamines / Q000737:chemistry; D005990:Glycerol / Q000737:chemistry; D020404:Glycerophospholipids / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D007377:Interleukin-3 / Q000378:metabolism; D008055:Lipids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D010743:Phospholipids / Q000737:chemistry; D010767:Phosphorylcholine / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D004983:Ethanolamines; D020404:Glycerophospholipids; D007377:Interleukin-3; D008055:Lipids; D010743:Phospholipids; D010767:Phosphorylcholine; D005990:Glycerol