title: Amylin causes anorexigenic effects via the hypothalamus and brain stem in chicks.
abstract: This study was conducted to determine the effects of amylin on appetite-related processes in chicks. Broiler chicks were centrally and peripherally injected with amylin, and feed and water intake were quantified. Feed intake was reduced after both central and peripheral amylin, but water intake was not affected. To determine if the hypothalamus and brainstem were involved in the anorexigenic effect, chicks were centrally and peripherally injected with amylin, and c-Fos immunoreactivity was quantified in the lateral hypothalamus (LH), ventromedial hypothalamus (VMH), area postrema (AP) and the nucleus of the solitary tract (NTS). Amylin decreased c-Fos immunoreactivity in the LH, did not affect the VMH, and increased c-Fos immunoreactivity in the AP and NTS. To determine if alimentary transit time was affected, chicks received central amylin and were gavaged with chicken feed slurry containing a visible marker. Amylin-treated chicks had increased alimentary canal transit time. Chicks also responded to central amylin with increased anxiety-related behaviors and increased plasma corticosterone concentration. These results demonstrate that amylin affects feeding, alimentary canal transit, and behavior through hypothalamic and brainstem mechanisms in chicks.
authors.affiliation: Department of Biology (6931), Radford University, Radford, Virginia 24142, USA. mcline@radford.edu
authors.forename: Mark A
authors.initials: MA
authors.lastname: Cline
authors.forename: Wint
authors.initials: W
authors.lastname: Nandar
authors.forename: Marissa L
authors.initials: ML
authors.lastname: Smith
authors.forename: Bryan H
authors.initials: BH
authors.lastname: Pittman
authors.forename: Mathew
authors.initials: M
authors.lastname: Kelly
authors.forename: J Orion
authors.initials: JO
authors.lastname: Rogers
mesh_terms: D000682:Amyloid / Q000494:pharmacology*; D000818:Animals; D000855:Anorexia / Q000139:chemically induced*; D001933:Brain Stem / Q000187:drug effects* / Q000502:physiology; D002645:Chickens; D003345:Corticosterone / Q000097:blood; D007031:Hypothalamus / Q000187:drug effects* / Q000502:physiology; D007150:Immunohistochemistry; D058228:Islet Amyloid Polypeptide; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism
chemical_list: D000682:Amyloid; D058228:Islet Amyloid Polypeptide; D016760:Proto-Oncogene Proteins c-fos; D003345:Corticosterone