title: Hypothalamic gene expression following ghrelin therapy to gastrectomized rodents.
abstract: We investigated whether ghrelin depletion (by gastrectomy surgery) and/or treatment/replacement with the gastric hormone ghrelin alters the expression of key hypothalamic genes involved in energy balance, in a manner consistent with ghrelin's pro-obesity effects. At 2 weeks after surgery mice were treated with ghrelin (12 nmol/mouse/day, sc) or vehicle for 8 weeks. Gastrectomy had little effect on the expression of these genes, with the exception of NPY mRNA in the arcuate nucleus that was increased. Ghrelin treatment (to gastrectomized and sham mice) increased the mRNA expression of orexigenic peptides NPY and AgRP while decreasing mRNA expression of the anorexigenic peptide POMC. Two weeks gavage treatment with the ghrelin mimetic, MK-0677, to rats increased NPY and POMC mRNA in the arcuate nucleus and MCH mRNA in the lateral hypothalamus. Thus, while predicted pro-obesity ghrelin signalling pathways were activated by ghrelin and ghrelin mimetics, these were largely unaffected by gastrectomy.
authors.affiliation: Department of Physiology/Endocrinology, Institute of Neuroscience and Physiology, The Sahlgrenska Academy at Göteborg University, P.O. Box 434, SE-405 30 Göteborg, Sweden.
authors.forename: Emil
authors.initials: E
authors.lastname: Egecioglu
authors.forename: Björn
authors.initials: B
authors.lastname: Stenström
authors.forename: Scarlett B
authors.initials: SB
authors.lastname: Pinnock
authors.forename: Loraine Y C
authors.initials: LY
authors.lastname: Tung
authors.forename: Charlotta
authors.initials: C
authors.lastname: Dornonville de la Cour
authors.forename: Andreas
authors.initials: A
authors.lastname: Lindqvist
authors.forename: Rolf
authors.initials: R
authors.lastname: Håkanson
authors.forename: Unni
authors.initials: U
authors.lastname: Syversen
authors.forename: Duan
authors.initials: D
authors.lastname: Chen
authors.forename: Suzanne L
authors.initials: SL
authors.lastname: Dickson
mesh_terms: D054369:Agouti-Related Protein / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D005260:Female; D005743:Gastrectomy; D005786:Gene Expression Regulation; D054439:Ghrelin / Q000494:pharmacology*; D007031:Hypothalamus / Q000378:metabolism*; D051379:Mice; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism*; D011333:Pro-Opiomelanocortin / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D013270:Stomach / Q000601:surgery*
chemical_list: D054369:Agouti-Related Protein; D054439:Ghrelin; D009478:Neuropeptide Y; D012333:RNA, Messenger; D011333:Pro-Opiomelanocortin