Proteomic analysis of two head and neck cancer cell lines presenting different radiation sensitivity.
CONCLUSIONS
We found four proteins in the AMC-HN-9 cells that might perform important functions in radio-resistance. These results also suggest that this cell line may provide us with important information about cancer cells regarding the roles of reactive oxygen species (ROS and antioxidants) in irradiation.


OBJECTIVE
Radiation susceptibility can be determined by the expression of some proteins. To identify such proteins involved in radiation susceptibility, we analyzed two cell lines with different radiation sensitivity.


METHODS
The AMC-HN-3 and -9 cell lines established from head and neck cancer patients were employed. They were irradiated with 4 Gy and two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) was carried out. Then matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) was performed to identify 20 proteins. Western blotting was used to confirm the significantly expressed proteins.


RESULTS
After 2-D PAGE, five spots were differentially expressed in the AMC-HN-9. Heat shock protein 27 kDa (HSP27), peroxiredoxin (Prx) II, glutathione S-transferase pi (GSTP), and an unknown protein were detected through MALDI-TOF MS. By using Western blotting, remarkable increments of the band intensity of HSP27, GSTP, Prx II, and Prx IV were confirmed in the AMC-HN-9 cell line.
Lee
Yoon Se
YS
Department of Otolaryngology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.
Chang
Hyo Won
HW
Jeong
Jeong Eun
JE
Lee
Sang-Wook
SW
Kim
Sang Yoon
SY
D000975:Antioxidants / Q000378:metabolism; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D051549:Glutathione S-Transferase pi / Q000032:analysis*; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000032:analysis*; D010039:Otorhinolaryngologic Neoplasms / Q000473:pathology* / Q000532:radiotherapy*; D054464:Peroxiredoxins / Q000032:analysis*; D040901:Proteomics; D011836:Radiation Tolerance / Q000528:radiation effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
D000975:Antioxidants; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D017382:Reactive Oxygen Species; D054464:Peroxiredoxins; D051549:Glutathione S-Transferase pi