Enhanced weak-signal sensitivity in two-photon microscopy by adaptive illumination.
We describe a technique to enhance both the weak-signal relative sensitivity and the dynamic range of a laser scanning optical microscope. The technique is based on maintaining a fixed detection power by fast feedback control of the illumination power, thereby transferring high measurement resolution to weak signals while virtually eliminating the possibility of image saturation. We analyze and demonstrate the benefits of adaptive illumination in two-photon fluorescence microscopy.
Chu
Kengyeh K
KK
Department of Biomedical Engineering, Boston University, MA 02215, USA.
Lim
Daryl
D
Mertz
Jerome
J
D000465:Algorithms; D005246:Feedback; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008029:Lighting / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D012680:Sensitivity and Specificity