Prenatal increased asymmetric dimethylarginine is associated with placental heat-shock protein 70 and lectin-like oxidized low-density lipoprotein receptor-1 expression.
BACKGROUND
Asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase, induces endothelial dysfunction by reversibly blocking NO production from L-arginine. To elucidate the association of prenatal status of ADMA with placental heat-shock protein 70 (HSP70) and lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in normal full-term pregnancies, we evaluated the expression of placental HSP70 and LOX-1.


METHODS
Tissue samples of placentas obtained from 60 normal-term pregnancies were categorized into 30 cases with a lower level of prenatal ADMA (0.28 +/- 0.07 microM) and 30 cases with a higher level of prenatal ADMA (1.31 +/- 0.44 microM). We evaluated the placental expression of HSP70 and LOX-1 with Western blot analysis and immunohistochemistry and determined the correlation between HSP70 and LOX-1.


RESULTS
We found that prenatal increased ADMA is associated with increased placental HSP70 and LOX-1 expression.


CONCLUSIONS
We postulate that higher ADMA levels are associated with excessive levels of oxidative damage and stress markers (HSP, LOX-1) in placental tissues.
Kim
Young Ju
YJ
Department of Obstetrics and Gynecology, School of Medicine, Ewha Womans University, Seoul, Korea. kkyj@ewha.ac.kr
Jeon
Yeo Jin
YJ
Ahn
Young Mo
YM
Lee
Hwa Young
HY
Woo
So Yeon
SY
Park
Hye Sook
HS
Ha
Eun Hee
EH
Pang
Myung Geol
MG
D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D015153:Blotting, Western; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D051127:Scavenger Receptors, Class E / Q000378:metabolism*; D047929:Term Birth / Q000097:blood / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D051127:Scavenger Receptors, Class E; D009569:Nitric Oxide; C024917:symmetric dimethylarginine; D001120:Arginine