title: Cyclin D1 and p16 expression in pleomorphic adenoma and carcinoma ex pleomorphic adenoma of the parotid gland.
abstract: OBJECTIVE
  To compare cyclin D1 and p16(ink4) (p16) expression in normal tissue, pleomorphic adenoma (PA) and carcinoma ex pleomorphic adenoma (CXPA) of the parotid gland.
  
  
  RESULTS
  Immunohistochemistry was used to examine cyclin D1 and p16 expression in 43 parotid tumours (29 PAs and 14 CXPAs). Cyclin D1 and p16 were both significantly more likely to be expressed in the neoplastic than in the normal epithelial and stromal components of PA and CXPA (P < 0.001 and P < 0.005, respectively). Cyclin D1 was more likely to be expressed in the malignant components of CXPA than in the benign components of PA (50% versus 31% and 31%, respectively), but the trend was not statistically significant. There was no evidence of this association for p16 (corresponding positivity rates 69% versus 81% and 52%).
  
  
  CONCLUSIONS
  Our findings provide preliminary evidence of roles for cyclin D1 and p16 in the development of PA and for cyclin D1 in the progression of PA to CXPA.
authors.affiliation: Sydney Head & Neck Cancer Institute, Sydney Cancer Centre, and University of Sydney, Gloucester House, Royal Prince Alfred Hospital, Camperdown, NSW, Australia. dr_rajan_patel@hotmail.com
authors.forename: R S
authors.initials: RS
authors.lastname: Patel
authors.forename: B
authors.initials: B
authors.lastname: Rose
authors.forename: H
authors.initials: H
authors.lastname: Bawdon
authors.forename: A
authors.initials: A
authors.lastname: Hong
authors.forename: C S
authors.initials: CS
authors.lastname: Lee
authors.forename: S
authors.initials: S
authors.lastname: Fredericks
authors.forename: K
authors.initials: K
authors.lastname: Gao
authors.forename: C J
authors.initials: CJ
authors.lastname: O'Brien
mesh_terms: D008949:Adenoma, Pleomorphic / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D056741:Cyclin D; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D016213:Cyclins / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010306:Parotid Gland / Q000378:metabolism* / Q000473:pathology; D010307:Parotid Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D056741:Cyclin D; D019941:Cyclin-Dependent Kinase Inhibitor p16; D016213:Cyclins