title: [Comparison of the binding affinity of LAIR-1(CD305) and LAIR-2(CD306) with their ligands].
abstract: OBJECTIVE
  To compare the binding affinity of LAIR-1 and LAIR-2 with their ligands.
  
  
  METHODS
  The ligand-expressing cells were incubated with the LAIR-1 and/or LAIR-2 fusion proteins at different concentration at the same time or in succession. Then the interaction changes and competition were measured by influorescent staining and flow cytometric analysis with the specific mAbs against LAIR-1 or LAIR-2.
  
  
  RESULTS
  The membrane ligand for LAIR-1 and LAIR-2 was expressed extensively and ligand recognition was of cross-species. LAIR-2 blocked the interaction of LAIR-1 with its ligand but LAIR-1 didn't block the interaction of LAIR-2 with its ligand.
  
  
  CONCLUSIONS
  LAIR-1 and LAIR-2 probably bind to the same ligand with different affinity, which provides some significant evidence for investigating the molecular mechanisms of LAIR-1 family in modulating immune response.
authors.affiliation: Transplantation Center, Second Affiliated Hospital, General Hospital of PLA, Beijing, China. xg_xu2000@yahoo.com
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Xu
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Xie
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei-Ning
authors.initials: WN
authors.lastname: Han
authors.forename: Ming
authors.initials: M
authors.lastname: Cai
authors.forename: Bing-Yi
authors.initials: BY
authors.lastname: Shi
authors.forename: Bo-Quan
authors.initials: BQ
authors.lastname: Jin
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006801:Humans; D008024:Ligands; D011485:Protein Binding; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D014162:Transfection
chemical_list: C431503:LAIR-2 receptor; D008024:Ligands; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins; C108073:leukocyte-associated immunoglobulin-like receptor 1