title: Functional analysis of type 1 isopentenyl diphosphate isomerase from Halobacterium sp. NRC-1.
abstract: Here we report the characterization of the type-1 isopentenyl diphosphate isomerase derived from Halobacterium sp. NRC-1. The expressed purified enzyme showed maximum isomerase activity in the presence of 1 M NaCl at 37 degrees C at pH 6.0. This type-1 enzyme appears to be the first for which the Co2+ ion is required for activity.
authors.affiliation: Department of Chemistry and Materials Science, Tokyo Institute of Technology, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hoshino
authors.forename: Tadashi
authors.initials: T
authors.lastname: Eguchi
mesh_terms: D019843:Archaeal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D019748:Carbon-Carbon Double Bond Isomerases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002413:Cations, Divalent / Q000378:metabolism; D002876:Chromosomes, Bacterial; D003035:Cobalt / Q000378:metabolism; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D005486:Flavin Mononucleotide / Q000378:metabolism; D018628:Gene Dosage; D005798:Genes, Bacterial; D006217:Halobacterium / Q000201:enzymology*; D045782:Hemiterpenes; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D019843:Archaeal Proteins; D002413:Cations, Divalent; D004269:DNA, Bacterial; D045782:Hemiterpenes; D011994:Recombinant Proteins; D009243:NAD; D003035:Cobalt; D012965:Sodium Chloride; D005486:Flavin Mononucleotide; D019748:Carbon-Carbon Double Bond Isomerases; C021453:isopentenyldiphosphate delta-isomerase