Simultaneous chiral resolution of monosaccharides as 8-aminonaphthalene-1,3,6-trisulfonate derivatives by ligand-exchange CE using borate as a central ion of the chiral selector.
Six reducing monosaccharides (mannose, galactose, fucose, glucose, xylose, and arabinose) were derivatized with 8-aminonaphthalene-1,3,6-trisulfonate (ANTS). Based on the chiral ligand-exchange principle using borate as a central ion of the chiral selector and (S)-3-amino-1,2-propanediol (SAP) as a chiral selector ligand, all of the six ANTS-monosaccharides were simultaneously enantioseparated using absorbance at 245 nm for detection. The optimum conditions for both high resolution and moderately short migration time consisted of 200 mM SAP-200 mM borate buffer (pH 9.2) containing 10% ACN as a BGE at 30 degrees C with an applied voltage of +30 kV. It was revealed that the proposed chiral ligand-exchange CE using the SAP-borate system was applicable to enantioseparation of not only diols but also polyols.
Kodama
Shuji
S
Toyama Institute of Health, Imizu, Toyama, Japan. shuji.kodama@pref.toyama.lg.jp
Aizawa
Sen-ichi
S
Taga
Atsushi
A
Yamashita
Tomohisa
T
Kemmei
Tomoko
T
Yamamoto
Atsushi
A
Hayakawa
Kazuichi
K
D001881:Borates / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D015394:Molecular Structure; D009005:Monosaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D009281:Naphthalenes / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism
D001881:Borates; D008024:Ligands; D009005:Monosaccharides; D009281:Naphthalenes; C046465:8-amino-1,3,6-naphthalenetrisulfonic acid