title: Cartilage gene expression correlates with radiographic severity of canine elbow osteoarthritis.
abstract: The relationship between radiographic measures of severity of osteoarthritis (OA) and changes in gene expression in joints are not well characterised. In this study, the expression of 11 candidate genes was characterised by quantitative reverse transcriptase polymerase chain reaction in normal and OA cartilage and bone from the elbows of dogs with fragmented coronoid disease. The levels of expression of type I collagen alpha2 chain (COL1A2), type III collagen alpha1 chain (COL3A1), lumican (LUM), matrix metalloproteinase-2 (MMP2), -9 (MMP9) and -13 (MMP13) genes were increased and the expression of tissue inhibitor of metalloproteinase-2 (TIMP2) and cathepsin D (CTSD) genes were decreased in OA cartilage relative to normal cartilage. All differences correlated with radiographic measures of severity of OA. Levels of expression of COL1A2, MMP2, MMP9, MMP13 and TIMP1 were increased, whereas expression of TIMP2 was decreased in OA bone relative to normal bone. Cartilage gene expression may be correlated with the radiographic severity of OA.
authors.affiliation: Department of Veterinary Pathology, University of Liverpool, Liverpool L69 3BX, UK. dylan.clements@ed.ac.uk
authors.forename: Dylan N
authors.initials: DN
authors.lastname: Clements
authors.forename: Noel
authors.initials: N
authors.lastname: Fitzpatrick
authors.forename: Stuart D
authors.initials: SD
authors.lastname: Carter
authors.forename: Philip J R
authors.initials: PJ
authors.lastname: Day
mesh_terms: D000818:Animals; D001483:Base Sequence; D002358:Cartilage, Articular / Q000378:metabolism*; D003094:Collagen / Q000235:genetics* / Q000378:metabolism; D004283:Dog Diseases / Q000000981:diagnostic imaging* / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004285:Dogs; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000662:veterinary; D045726:Metalloproteases / Q000096:biosynthesis / Q000235:genetics; D010003:Osteoarthritis / Q000000981:diagnostic imaging / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D011509:Proteoglycans / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D011859:Radiography; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012720:Severity of Illness Index
chemical_list: D011509:Proteoglycans; D012333:RNA, Messenger; D003094:Collagen; D045726:Metalloproteases