Proteome changes in Caco-2 cells treated with Monascus-fermented red mold rice extract.
Monascus-fermented red mold rice has been extensively used as a folk medicine for thousands of years. Monascus secondary metabolites, including monacolin K, monascorubrin, and ankaflavin, have been reported to have an antiproliferative effect on cancer cells. However, the cell machinery responsible for the antiproliferation of Monascus-fermented red mold rice treatment in cancer cells remains unclear. A proteomic approach using two-dimensional gel electrophoresis, matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry, and tandem mass spectrometry was used to identify proteins with modified expression in Caco-2 cells treated with Monascus-fermented red mold rice extract. A total of 20 proteins were identified with significantly altered expression (P < 0.05) in response to Monascus-fermented red mold rice extract treatment. The deregulated proteins that were identified included heat shock protein 70, protein kinase C epsilon type, clusterin-associated protein 1, and two tumor suppressors (N-chimaerin and calponin-2). Our results suggested the involvement of heat shock protein 70-mediated cytotoxicity in the Caco-2 cells treated with Monascus-fermented red mold rice extract.
Lin
Wun-Yuan
WY
Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University, Taiwan, Republic of China.
Hsu
Wei-Yi
WY
Hish
Chih-Hsuan
CH
Pan
Tzu-Ming
TM
D000595:Amino Acid Sequence; D018938:Caco-2 Cells; D002455:Cell Division / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D005285:Fermentation; D006801:Humans; D008969:Molecular Sequence Data; D032741:Monascus / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D012275:Oryza / Q000737:chemistry* / Q000382:microbiology; D010936:Plant Extracts / Q000494:pharmacology*; D020543:Proteome / Q000032:analysis*
D009363:Neoplasm Proteins; D010936:Plant Extracts; D020543:Proteome