title: Comparative analysis of the protein profiles from primary gastric tumors and their adjacent regions: MAWBP could be a new protein candidate involved in gastric cancer.
abstract: The study of tumor biomarkers is generally facilitated by the adoption of proteomic strategies. With limitations of techniques and individual varieties of biological samples, the biomarkers for gastric cancer (GC) have not reached a common agreement derived from the proteomic investigations. Herein, we reported a new set of data for screening the biomarkers from the gastric tissues, on the basis of the proteomic strategy developed in our laboratory. Ten pairs of the clinic samples were collected and treated with protein extraction. The gastric proteins were well-resolved by 2-DE, and the GC-associated proteins were identified by MALDI-TOF/TOF MS following image analysis, including 12 up-regulated and 13 down-regulated unique ones. MAWBP was found to be one of the new GC proteins which appeared with lower expression in the GC tissues. We expanded a systematical examination to deeply pursue the relevance between MAWBP and GC. Quantitatively, we measured the expression of MAWBP with Western blot and Real-Time PCR. Extendedly, we estimated the existence of MAWBP with immunohistochemical staining in a large number of the GC cases. Specifically, we inquired whether MAWD, a protein with high affinity to MAWBP, could coexpress and interact with MAWBP in vivo. On the basis of all the results, we concluded that MAWBP could be a new GC-related protein even though its physiological roles remain unexplored.
authors.affiliation: Beijing Genomics Institute, Chinese Academy of Sciences, Beijing Airport Industrial Zone B-6, Shunyi, Beijing, China 101318.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Kang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Tan
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Bai
authors.forename: Yumei
authors.initials: Y
authors.lastname: Liang
authors.forename: Rui
authors.initials: R
authors.lastname: Xing
authors.forename: Jianmin
authors.initials: J
authors.lastname: Shao
authors.forename: Ningzhi
authors.initials: N
authors.lastname: Xu
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Siqi
authors.initials: S
authors.lastname: Liu
authors.forename: Youyong
authors.initials: Y
authors.lastname: Lu
mesh_terms: D018076:DNA, Complementary / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry / Q000379:methods; D008099:Liver / Q000378:metabolism; D013058:Mass Spectrometry; D020411:Oligonucleotide Array Sequence Analysis; D017434:Protein Structure, Tertiary; D040901:Proteomics / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology*
chemical_list: D018076:DNA, Complementary