Simultaneous determination of Angiotensin I-converting enzyme inhibitory peptides in tryptic casein hydrolysate by high-performance liquid chromatography combined with a replicate heart-cut column-switching technique.
A replicate heart-cut column-switching HPLC method combined with two switching valves was newly developed for the simultaneous determination of three antihypertensive peptides (Ala-Phe, Tyr-Pro, and Trp-Tyr) in tryptic casein hydrolysate in one run-in assay. After a first separation on an octadecyl silane (ODS) column, heart-cuts of each peptide were individually separated on a subsequent analytical ODS column: 26% acetonitrile for Ala-Phe and Tyr-Pro (32% for Trp-Tyr) in 0.1% trifluoroacetic acid containing 10 mM sodium 1-octanesulfonate at 0.8 mL/min. Ala-Phe, Tyr-Pro, and Trp-Tyr in casein hydrolysate were determined within 70 min to be 0.377 +/- 0.037 mg/g, 2.50 +/- 0.26 mg/g, and 0.096 +/- 0.008 mg/g, respectively.
Zhu
Xiao-Lin
XL
Division of Bioscience and Bioenvironmental Sciences, Faculty of Agriculture, Graduate School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
Matsui
Toshiro
T
Watanabe
Keisuke
K
Shiraishi
Koso
K
Ueki
Tatsuro
T
Matsumoto
Kiyoshi
K
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis*; D002364:Caseins / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004151:Dipeptides / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D014357:Trypsin
D000806:Angiotensin-Converting Enzyme Inhibitors; D002364:Caseins; D004151:Dipeptides; C120594:tyrosyl-proline; C029951:tryptophyltyrosine; C014309:alanylphenylalanine; C475729:casein hydrolysate; D014357:Trypsin