Effect of Angelica gigas extract on melanogenesis in B16 melanoma cells.
During the screening of herbs for inhibition of melanogenesis, it was observed that ethanolic extract of Angelicae Gigantis Radix (AGE) effectively inhibited isobutylmethylxanthine-induced melanogenesis in B16 melanoma cells. The melanin content was significantly decreased by AGE in a dose-dependent manner, and no cytotoxicity was observed at the effective concentrations. Decreased melanin content was accompanied by reduced enzyme activity as well as reduced expression of tyrosinase protein and mRNA. The level of tyrosinase-related protein 1 and 2 mRNAs was also decreased by AGE. Additionally, AGE effectively inhibited alpha-melanocyte stimulating hormone- and forskolin-induced melanogenesis, and downregulated the mRNA expression of microphthalmia-associated transcription factor, a master transcriptional regulator of melanogenic genes. These results suggest that AGE acts as a putative hypopigmenting agent through downregulation of tyrosinase expression induced via a cAMP-dependent pathway.
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Department of Biochemistry, Medical School and Institute for Medical Sciences, Chonbuk National University, Jeonju, Jeonbuk 561-756, Korea.
Koo
Jeung-Hyun
JH
Yoon
Ha-Yong
HY
Yu
Jiahua
J
Kim
Kyung-Ah
KA
Choi
Il-Whan
IW
Kwon
Kang-Beom
KB
Kwon
Keun-Sang
KS
Kim
Han-Uk
HU
Park
Jin-Woo
JW
Park
Byung-Hyun
BH
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D019661:Apiaceae / Q000737:chemistry*; D016923:Cell Death / Q000187:drug effects; D005576:Colforsin / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D008543:Melanins / Q000096:biosynthesis*; D008545:Melanoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000521:alpha-MSH / Q000378:metabolism
D008543:Melanins; D010936:Plant Extracts; D012333:RNA, Messenger; D005576:Colforsin; D000521:alpha-MSH; D014442:Monophenol Monooxygenase; D015056:1-Methyl-3-isobutylxanthine