Actin protein up-regulated upon infection and development of the filarial parasite, Wuchereria bancrofti (Spirurida: Onchocercidae), in the vector mosquito, Culex quinquefasciatus (Diptera: Culicidae).
Detection and identification of humoral proteins, which are up-regulated in Culex quinquefasciatus upon infection by Wuchereria bancrofti, is important in tracing out the biochemical consequences of the filarial parasite development in the vector mosquito. Analysis of the haemolymph of infected mosquitoes through SDS-PAGE and RP-HPLC showed up-regulation of five proteins of molecular weights 40, 66, 22, 14, and 7-kDa. Among these, only the 40-kDa was unknown and the others were comparable with those already reported as transferrin, attacin, lysozyme, and defensin, respectively. In the present study, the 40-kDa protein up-regulated upon infection was identified as actin through nano-LC-MS/MS analysis. Actin is known to be one of the cytoskeletal proteins up-regulated in the haemolymph, as part of the innate immune system, of Escherichia coli challenged Drosophila melanogaster larvae. For the first time, we have observed an increased level of actin in the haemolymph of W. bancrofti-infected Cx. quinquefasciatus. However, the exact mechanism of actin involvement in the immune system of this mosquito is yet to be studied.
Agiesh Kumar
B
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Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, Pondicherry 605006, India.
Paily
K P
KP
D000199:Actins / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D003465:Culex / Q000378:metabolism / Q000469:parasitology*; D023082:Defensins / Q000302:isolation & purification / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006458:Hemolymph / Q000737:chemistry / Q000378:metabolism; D019476:Insect Proteins / Q000302:isolation & purification / Q000378:metabolism; D007303:Insect Vectors / Q000378:metabolism / Q000469:parasitology*; D009113:Muramidase / Q000302:isolation & purification / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D014168:Transferrin / Q000302:isolation & purification / Q000378:metabolism; D015854:Up-Regulation; D014958:Wuchereria bancrofti / Q000502:physiology*
D000199:Actins; D023082:Defensins; D019476:Insect Proteins; D014168:Transferrin; C039711:attacin antibacterial protein, insect; D009113:Muramidase