Effect of various calcium/phosphates ratios of carboxymethylcellulose-based saliva substitutes on mineral loss of bovine enamel in vitro.
OBJECTIVE
The present study evaluated the effects of various calcium and phosphate concentrations and ratios of carboxymethylcellulose (CMC)-based solutions on the mineral loss of predemineralised bovine enamel in vitro.


METHODS
Bovine enamel specimens were prepared, polished and partly covered with nail varnish, thus serving as control of sound enamel. After demineralisation (37 degrees C; pH 5.0; 14 days) the specimens were exposed to CMC-based solutions (20g/l) with various saturations with respect to apatites containing 0.1mM NaF, CaCl2 (0-32 mM) and KH2PO4 (0-52 mM) at two different pH values (5.5 or 6.5). A fluoride-free solution served as control, and four commercially available products were tested as well. The differences in mineral loss (DeltaDeltaZ) between the values prior to (DeltaZ Demin) and after storage (DeltaZ Effect) in the various solutions were evaluated from microradiographs of thin sections (100microm).


RESULTS
The general linear model revealed a significant dependency for DeltaDeltaZ on 'calcium' (p<0.001), 'phosphate' (p=0.023), 'fluoride' (p=0.002) and 'pH' (p<0.001). With increasing calcium and phosphate concentrations an increase in DeltaDeltaZ could be observed up to the solution containing the third highest saturation with respect to octacalciumphosphate (3.2), showing a significant remineralisation (p<0.05; t-test). The commercially available products as well as the control groups revealed significantly reduced DeltaDeltaZ values compared to this group (p<0.01; Bonferroni).


CONCLUSIONS
A saturation with respect to octacalciumphosphate of 3.2 and a pH of 6.5 enables CMC-based solutions to remineralise bovine enamel in vitro.
Meyer-Lueckel
Hendrik
H
Department of Operative Dentistry and Periodontology, University School of Dental Medicine, Campus Benjamin Franklin, Charité - Universitätsmedizin Berlin, Germany.
Chatzidakis
Andreas J
AJ
Kielbassa
Andrej M
AM
D000818:Animals; D002118:Calcium / Q000008:administration & dosage* / Q000032:analysis; D002122:Calcium Chloride / Q000008:administration & dosage; D002266:Carboxymethylcellulose Sodium / Q000008:administration & dosage*; D002417:Cattle; D003743:Dental Enamel / Q000187:drug effects*; D005459:Fluorides / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008903:Minerals / Q000032:analysis; D010710:Phosphates / Q000008:administration & dosage / Q000032:analysis; D010758:Phosphorus / Q000008:administration & dosage*; D017680:Potassium Compounds / Q000008:administration & dosage; D012464:Saliva, Artificial / Q000008:administration & dosage*; D012969:Sodium Fluoride / Q000008:administration & dosage; D017001:Tooth Demineralization / Q000628:therapy; D014089:Tooth Remineralization
D008903:Minerals; D010710:Phosphates; D017680:Potassium Compounds; D012464:Saliva, Artificial; D010758:Phosphorus; D012969:Sodium Fluoride; C013216:potassium phosphate; D002266:Carboxymethylcellulose Sodium; D002122:Calcium Chloride; D005459:Fluorides; D002118:Calcium