title: The use of naphthalene-2,3-dicarboxaldehyde for the analysis of primary amines using high-performance liquid chromatography and capillary electrophoresis.
abstract: This paper reviews analytical methods, instrumental developments and applications for derivatization of primary amines with naphthalene-2,3-dicarboxaldehyde using fluorescence and chemiluminescence detection with capillary electrophoresis (CE) and high performance liquid chromatography (HPLC). The use of lasers as well as lamps as the excitation source for fluorescence detection is discussed. The detection limit observed with naphthalene-2,3-dicarboxaldehyde derivatization is often lower and better than those obtained with other analytical separations and other fluorescent dyes. In addition, this paper describes the crucial points that influence the stability of NDA primary amine derivatives, and summarize the separation, derivatization and migration conditions of the different techniques, with their advantages and drawbacks.
authors.affiliation: Université Paul Sabatier, IMRCP, UMR 5623, Toulouse, France.
authors.forename: Georges
authors.initials: G
authors.lastname: Rammouz
authors.forename: Marlène
authors.initials: M
authors.lastname: Lacroix
authors.forename: Jean Christophe
authors.initials: JC
authors.lastname: Garrigues
authors.forename: Véréna
authors.initials: V
authors.lastname: Poinsot
authors.forename: François
authors.initials: F
authors.lastname: Couderc
mesh_terms: D000588:Amines / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008163:Luminescent Measurements; D009281:Naphthalenes / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000588:Amines; D005456:Fluorescent Dyes; D009281:Naphthalenes; C027717:2,3-naphthalenedicarboxaldehyde