title: Enhancement of europium emission band of europium tetracycline complex in the presence of cholesterol.
abstract: We report here the observation, for the first time, of the enhancement of Europium-Tetracycline complex emission in cholesterol solutions. This enhancement was initially observed with the addition of the enzyme cholesterol oxidase, which produces H(2)O(2), the agent driver of the Europium tetracycline complex, to the solution. However, it was found that the enzyme is not needed to enhance the luminescence. A calibration curve was determined, resulting in a simple method to measure the cholesterol quantity in a solution. This method shows that the complex can be used as a sensor to determine cholesterol in biological systems.
authors.affiliation: LSI, Escola Politécnica, Universidade de São Paulo, USP, São Paulo, SP, Brazil.
authors.forename: Flávia Rodrigues de Oliveira
authors.initials: FR
authors.lastname: Silva
authors.forename: Ricardo Elgul
authors.initials: RE
authors.lastname: Samad
authors.forename: Laércio
authors.initials: L
authors.lastname: Gomes
authors.forename: Lilia Coronato
authors.initials: LC
authors.lastname: Courrol
mesh_terms: D002784:Cholesterol / Q000378:metabolism*; D002789:Cholesterol Oxidase / Q000378:metabolism; D005063:Europium / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D049449:Luminescence; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013752:Tetracycline / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D005063:Europium; D002784:Cholesterol; D002789:Cholesterol Oxidase; D013752:Tetracycline