Distribution of olfactory marker protein on a tissue section of vomeronasal organ measured by AFM.
Distribution of olfactory marker protein (OMP) on a tissue section of vomeronasal organ (VNO) was successfully measured by atomic force microscopy (AFM). Anti-OMP antibodies were covalently crosslinked with the tip of the AFM and were used as a probe to observe the distribution of OMP on a tissue section. First, force measurements were performed using a glass surface on which OMP was covalently immobilized to verify the success of tip modification. Clear differences of interaction forces were observed between a specific pair and the control experiments, indicating that the tip preparation succeeded. Next, distributions of OMP on the tissue section were observed by AFM and were compared with immunohistochemical observations. For large scale observation, a microbead was used as a probe in the AFM measurements. The results of the AFM measurements were well overlapped with that of immunohistochemistry, confirming the reliability of our method. A mapping of the AFM measurement with high resolution was also successfully obtained, which showed an advantage of the application of the AFM measurement in analysis of proteins on the tissue section.
Kim
Hyonchol
H
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama, Japan. kim.bmi@tmd.ac.jp
Asgari
Firoozeh
F
Kato-Negishi
Midori
M
Ohkura
Satoshi
S
Okamura
Hiroaki
H
Arakawa
Hideo
H
Osada
Toshiya
T
Ikai
Atsushi
A
D000818:Animals; D000906:Antibodies; D001696:Biomechanical Phenomena; D006041:Goats; D007150:Immunohistochemistry; D018625:Microscopy, Atomic Force; D051842:Olfactory Marker Protein / Q000378:metabolism* / Q000648:ultrastructure*; D019147:Vomeronasal Organ / Q000378:metabolism* / Q000648:ultrastructure*
D000906:Antibodies; D051842:Olfactory Marker Protein