title: Rapid kinetic microassay for catalase activity.
abstract: Catalase is a commonly assayed enzyme found in many bacteria and eukaryotes. In this report, we examined the applicability of a kinetic microassay to quantify catalase from two different sources. The assay was found to be linear over a wide range (0.1-1.0 units), but was limited at high values (>1 unit) by oxygen evolution. Nonetheless, the microassay allows simultaneous evaluation of many samples (up to 96) in a short time (<5 min) and is thus well-suited to applications, such as high-throughput screening, where many parallel assays are required.
authors.affiliation: Life Sciences Building 433, 1280 Main Street West, Hamilton, Ontario, Canada.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Herb E
authors.initials: HE
authors.lastname: Schellhorn
mesh_terms: D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D012680:Sensitivity and Specificity
chemical_list: D002374:Catalase