The NADPH quinone reductase MdaB confers oxidative stress resistance to Helicobacter hepaticus.
An mdaB mutant strain in a quinone reductase (MdaB) of Helicobacter hepaticus type strain ATCC51449 was constructed by insertional mutagenesis, and the MdaB protein was purified and compared to the Helicobacter pylori enzyme. While wild type H. hepaticus cells could tolerate 6% O(2) for growth, the mdaB strain was clearly inhibited at this oxygen level. Disruption of the gene downstream of mdaB (HH1473) did not affect the oxidative stress phenotype of the strain. The mdaB mutant was also more sensitive to oxidative stress reagents such as H(2)O(2), cumene hydroperoxide, t-butyl hydroperoxide, and paraquat. All H. hepaticus mdaB strains isolated constitutively up-expressed another oxidative stress-combating enzyme, superoxide dismutase; this is in contrast to H. pylori mdaB strains. H. hepaticus MdaB is a flavoprotein catalyzing quinone reduction using a two-electron transfer mechanism from NAD(P)H to quinone. The H. hepaticus enzyme specific activity was far less than for the H. pylori enzyme purified in the same manner.
Hong
Yang
Y
Department of Microbiology, University of Georgia, Athens, GA 30602, USA.
Wang
Ge
G
Maier
Robert J
RJ
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001555:Benzene Derivatives / Q000494:pharmacology; D017353:Gene Deletion; D044923:Helicobacter hepaticus / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology*; D016480:Helicobacter pylori / Q000201:enzymology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007700:Kinetics; D016254:Mutagenesis, Insertional; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D018384:Oxidative Stress; D010100:Oxygen / Q000494:pharmacology; D010269:Paraquat / Q000494:pharmacology; D020543:Proteome / Q000032:analysis; D013482:Superoxide Dismutase / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001555:Benzene Derivatives; D020543:Proteome; D009249:NADP; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D016660:NAD(P)H Dehydrogenase (Quinone); C007164:cumene hydroperoxide; D010269:Paraquat; D010100:Oxygen