title: Microarray-based genomic selection for high-throughput resequencing.
abstract: We developed a general method, microarray-based genomic selection (MGS), capable of selecting and enriching targeted sequences from complex eukaryotic genomes without the repeat blocking steps necessary for bacterial artificial chromosome (BAC)-based genomic selection. We demonstrate that large human genomic regions, on the order of hundreds of kilobases, can be enriched and resequenced with resequencing arrays. MGS, when combined with a next-generation resequencing technology, can enable large-scale resequencing in single-investigator laboratories.
authors.affiliation: Department of Human Genetics, Emory University School of Medicine, 615 Michael Street, Suite 301, Atlanta, Georgia 30322, USA.
authors.forename: David T
authors.initials: DT
authors.lastname: Okou
authors.forename: Karyn Meltz
authors.initials: KM
authors.lastname: Steinberg
authors.forename: Christina
authors.initials: C
authors.lastname: Middle
authors.forename: David J
authors.initials: DJ
authors.lastname: Cutler
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Albert
authors.forename: Michael E
authors.initials: ME
authors.lastname: Zwick
mesh_terms: D000818:Animals; D005057:Eukaryotic Cells / Q000378:metabolism; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000235:genetics; D015723:Gene Library; D016678:Genome / Q000235:genetics*; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods*; D015534:Trans-Activators / Q000235:genetics
chemical_list: D051860:Fragile X Messenger Ribonucleoprotein 1; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D015534:Trans-Activators; C099930:AFF2 protein, human; C497683:FMR1 protein, human