Changing of antioxidant enzyme activity on the biliary obstructed rats treated with resveratrol.
The aim of this study was to evaluate the role of antioxidant enzyme activity and nitric oxide levels induced by 28 day biliary obstruction in the rat. A total of 21 young Swiss albino rats were divided in to 3 groups. Bile duct ligations, bile duct ligations plus resveratrol, sham operated. Bile duct ligations plus resveratrol group received 10 mg/kg dose of resveratrol intraperitonealy once daily throughout for 28 days. Liver damage and cholestasis were determined by biochemical examination. SOD, CAT and GSH-PX activity decreased in BDL group compared with shame opareted groups (p < 0.001). NO levels increased in BDL groups compared with shame opareted groups (p < 0.001). SOD, CAT and GSH-PX activity was found higher in BDL+resveratrol treated groups than BDL groups (p < 0.001). In addition this NO levels decreased in BDL+resveratrol treated groups than BDL groups (p < 0.001). In conclusion, it is thought that resveratrol may be used as a protective agent in biliary obstructions; however, further clinical and experimental studies are needed to verify its antioxidative and hepatoprotective effects.
Cenesiz
S
S
Department of Biochemistry, Faculty of Veterinary Medicine, Ondokuzmayis University, Samsun, Turkey. scenesiz@omu.edu.tr
Yarim
G F
GF
Karabulut
A B
AB
Ara
C
C
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001652:Bile Ducts / Q000601:surgery; D001663:Bilirubin / Q000097:blood; D002779:Cholestasis / Q000188:drug therapy / Q000201:enzymology / Q000662:veterinary*; D004912:Erythrocytes / Q000201:enzymology; D008026:Ligation / Q000662:veterinary; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008297:Male; D009569:Nitric Oxide / Q000097:blood; D010088:Oxidoreductases / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D000077185:Resveratrol; D013267:Stilbenes / Q000627:therapeutic use*
D000975:Antioxidants; D013267:Stilbenes; D009569:Nitric Oxide; D010088:Oxidoreductases; D000077185:Resveratrol; D001663:Bilirubin