Isolation and characterization of a novel helicobacter species, "Helicobacter macacae," from rhesus monkeys with and without chronic idiopathic colitis.
Chronic idiopathic colitis is a common clinical entity in young captive rhesus monkeys. Eight isolates, cultured from five monkeys in colony 1 with endemic diarrhea and three from colony 2 without diarrhea, were grown under microaerobic conditions on selective agar and were classified by full 16S rRNA sequence, biochemical, and phenotypic analysis as a novel helicobacter, "Helicobacter macacae" (proposed name). All eight strains of H. macacae had 99.5% identical 16S rRNA sequences.
Fox
James G
JG
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. jgfox@mit.edu
Boutin
Samuel R
SR
Handt
Laurence K
LK
Taylor
Nancy S
NS
Xu
Shilu
S
Rickman
Barry
B
Marini
Robert P
RP
Dewhirst
Floyd E
FE
Paster
Bruce J
BJ
Motzel
Sherri
S
Klein
Hilton J
HJ
D000818:Animals; D015373:Bacterial Typing Techniques; D003092:Colitis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology; D019353:Endemic Diseases; D020459:Genes, rRNA; D016998:Helicobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008253:Macaca mulatta / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S