Evaluation of platelet and leukocyte functions in effort angina patients using high shear conditions in small-sized collagen bead columns.
This study used a small-sized collagen bead column system to investigate platelet functions under high shear stress in 28 patients with effort angina and 15 healthy controls. Soluble P-selectin and soluble L-selectin were also evaluated. Patients underwent stent insertion, followed by treatment with aspirin (100 mg/day) and ticlopidine (200 mg/day). High-shear-dependent platelet function was measured using small-sized collagen beads. The retention rate of platelets from patients with angina was higher than that in healthy controls (10.9% +/- 3.9% versus 5.6% +/- 1.7%, P < .01). Soluble P-selectin and soluble L-selectin levels of patients with angina significantly increased after passage through the columns. Levels of soluble P-selectin and L-selectin in healthy controls did not exhibit significant changes with passage through the columns. These results suggested that high shear stress caused abnormal activation of leukocytes or adhesive proteins in patients with effort angina, and ticlopidine failed to suppress hyperaggregability of platelets in these patients.
Inami
Norihito
N
Second Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
Nomura
Shosaku
S
Kimura
Yutaka
Y
Yamada
Kohichi
K
Nakamori
Hisato
H
Takahashi
Nobuyuki
N
Fukuhara
Shirou
S
Iwasaka
Toshiji
T
D000368:Aged; D000787:Angina Pectoris / Q000097:blood* / Q000188:drug therapy / Q000628:therapy; D001241:Aspirin / Q000627:therapeutic use; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D016022:Case-Control Studies; D003094:Collagen; D005260:Female; D018056:Hemorheology; D006801:Humans; D066298:In Vitro Techniques; D019041:L-Selectin / Q000097:blood; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood; D010316:Particle Size; D010975:Platelet Aggregation Inhibitors / Q000627:therapeutic use; D010979:Platelet Function Tests / Q000379:methods*; D012995:Solubility; D015607:Stents; D013988:Ticlopidine / Q000627:therapeutic use
D019007:P-Selectin; D010975:Platelet Aggregation Inhibitors; D019041:L-Selectin; D003094:Collagen; D013988:Ticlopidine; D001241:Aspirin