title: [JAK3 mRNA level in graft versus host disease patients: a preliminary study].
abstract: OBJECTIVE
  To quantify peripheral blood mononuclear cell JAK3 mRNA levels after allogeneic haematopoietic stem cell (allo-HSCT) transplantation, and to explore its relationship with graft vs host disease (GVHD).
  
  
  METHODS
  Serial monitoring of JAK3 mRNA levels by fluorescent quantitative reverse transcriptase polymerase chain reaction (FQ-RT-PCR) technique was performed on 26 cases (83 samples) after allo-HSCT.
  
  
  RESULTS
  The mean JAK3/ABL expression levels was 8.71 in 14 patients without GVHD; the JAK3/ABL expression level was 31.94 in one patient with acute GVHD (aGVHD); the mean JAK3/ABL expression levels was 19.23 in 11 patients with chronic GVHD (cGVHD), and the cGVHD was diagnosed at the mean time of six months (3 - 12 months). The JAK3/ABL expression levels did not change in 4 patients one month after allo-HSCT, however, the levels increased again when GVHD were diagnosed in these 4 patients, and the JAK3/ABL expression levels decreased again when GVHD was remitted.
  
  
  CONCLUSIONS
  The JAK3 expression level related to the remission and relapse in patients with GVHD.
authors.affiliation: Department of Hematology, Guangdong Provincial People's Hospital, Guangzhou 510080, China.
authors.forename: Qi-hui
authors.initials: QH
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Du
authors.forename: Su-xia
authors.initials: SX
authors.lastname: Geng
authors.forename: Jian-yu
authors.initials: JY
authors.lastname: Weng
authors.forename: Rong
authors.initials: R
authors.lastname: Guo
authors.forename: Ze-sheng
authors.initials: ZS
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
authors.forename: Zi-lun
authors.initials: ZL
authors.lastname: Huang
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D015870:Gene Expression; D006086:Graft vs Host Disease / Q000235:genetics*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D053616:Janus Kinase 3 / Q000235:genetics*; D008297:Male; D036102:Peripheral Blood Stem Cell Transplantation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D053616:Janus Kinase 3