title: Bonding of acid-etch and self-etch adhesives to human fluorosed dentine.
abstract: OBJECTIVE
  The objective of our laboratory study was to determine the bonding efficacy of two-step self-etching primer system (Clearfil SE Bond), all-in-one adhesive (Clearfil Tri S Bond) and acid-etching system (Single Bond) to human fluorosed dentine.
  
  
  METHODS
  Forty-eight human molars were grouped according to modified Thylstrup-Fejerskov index (TFI) into normal (N, TFI 0), mild fluorosis (ML, TFI 1-3) and moderate fluorosis (MD, TFI 4-6). Superficial dentine was exposed and ground with 600-grit silicon carbide paper. Teeth of each group were randomly divided into three sub-groups. Each sub-group was treated with one of the three bonding systems. A resin composite was built up on the bonded surfaces and stored in water at 37 degrees C for 24h. They were serially sectioned in buccal-lingual direction into 0.7-1.0mm thick slabs. Slices were trimmed for microtensile bond test and stressed in tension at a crosshead speed of 1mm/min. The data were analyzed with two-way ANOVA (p=0.05). The adhesive/dentine interfaces of the bonded specimens were examined by a FE-SEM.
  
  
  RESULTS
  Significantly higher microtensile bond strength (microTBS) was found with Clearfil SE Bond to mild fluorosed dentine than did Single Bond and Clearfil Tri S Bond. Lower microTBS were found for Single Bond (not significant) and Clearfil Tri S Bond than Clearfil SE Bond with moderately fluorosed dentine. Thickness of the hybrid layer produced by Clearfil SE Bond in mild and moderately fluorosed groups was less than with normal dentine.
  
  
  CONCLUSIONS
  Two-step self-etching system, Clearfil SE Bond, showed a higher bonding performance to fluorosed dentine than Single Bond and Tri S Bond.
authors.affiliation: Department of Restorative Sciences, Graduate school, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan. kanchanapgkw@yahoo.com
authors.forename: P G K
authors.initials: PG
authors.lastname: Waidyasekera
authors.forename: T
authors.initials: T
authors.lastname: Nikaido
authors.forename: D D S
authors.initials: DD
authors.lastname: Weerasinghe
authors.forename: J
authors.initials: J
authors.lastname: Tagami
mesh_terms: D000134:Acid Etching, Dental; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D017554:Carbon Compounds, Inorganic / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding / Q000379:methods*; D003804:Dentin / Q000473:pathology*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D009050:Fluorosis, Dental / Q000473:pathology*; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D019279:Resin Cements / Q000737:chemistry*; D017655:Silicon Compounds / Q000737:chemistry; D013314:Stress, Mechanical; D013499:Surface Properties; D013696:Temperature; D013718:Tensile Strength; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D017554:Carbon Compounds, Inorganic; C438313:Clearfil SE Bond; C515604:Clearfil Tri-S Bond; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D019279:Resin Cements; D017655:Silicon Compounds; C115612:single bond; D014867:Water; D017438:Bisphenol A-Glycidyl Methacrylate; C022088:silicon carbide