The linkage of neural progenitor cell cycle profiles between embryonic and adult stroke models: Analytical approach II.
Cell kinetics employed for embryonic models was modified and used to study the neuronogenesis in the subventricular zone (SVZ) in adult rats subjected to stroke. Enhanced analytical approaches were introduced and used to compare the cell cycle length (T(C)) and length in G(1) phase, T(G1), at various times after stroke to study the correlation between T(G1) and T(C) and to compare cell cycle evolution and proliferation profiles between the stroke and embryonic models. Our data indicate that cell cycle kinetics for the embryonic model can be applied to stroke in the adult. Significant reduction of T(G1) early after stroke (p<0.05) corresponds to an increase of neural progenitor cells remaining in the cycle at early times and cells exiting at later times. T(G1) correlates with T(C) (r=0.99, p<0.05). In conclusion, the analytical approaches proposed can be used to study the cell proliferation profiles in adult rats subjected to stroke with and without stroke therapy. The cell kinetics the cell proliferation profile differs between the stroke and embryonic models. T(C) evolution is three-fold slower in the cells and leave the cycle earlier and more frequently in the stroke model, compared to the embryonic model. T(C) is a surrogate measure of T(G1).
Lu
Mei
M
Department of Biostatistics and Research Epidemiology, Henry Ford Hospital, One Ford Place, Ste. 3E, Detroit, MI 48202, USA. mlu1@hfhs.org
Zhang
Rui Lan
RL
Zhang
Zheng Gang
ZG
Chopp
Michael
M
D000367:Age Factors; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division; D049109:Cell Proliferation; D004195:Disease Models, Animal; D004622:Embryo, Mammalian; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology*; D020547:Lateral Ventricles / Q000473:pathology; D008297:Male; D008959:Models, Neurological; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000502:physiology*
D001973:Bromodeoxyuridine