Immunization with radioattenuated yeast cells of Paracoccidioides brasiliensis induces a long lasting protection in BALB/c mice.
Paracoccidioides brasiliensis is the fungus agent of paracoccidioidomycosis, a chronic systemic disease prevalent in Latin America. The aim of the present work was to evaluate the protection elicited by the immunization of BALB/c mice with radioattenuated yeast cells of P. brasiliensis. The immunization promoted a long lasting protection against highly infective yeast forms of P. brasiliensis. A 99.5% decrease in CFUs recovery was verified 90 days post challenge. At the same time the levels of IgG2a and IFN-gamma were high while a very low production of IL-10 and IL-5 was verified, suggesting that a Th1 pattern was dominant. This work shows the potential of radioattenuated yeast cells for the development of vaccines against fungi infections.
do Nascimento Martins
Estefânia Mara
EM
Laboratório de Radiobiologia, Centro de Desenvolvimento da Tecnologia Nuclear, Caixa postal 941, CEP 30123-970, Belo Horizonte, MG, Brazil. estefaniabio@yahoo.com.br <estefaniabio@yahoo.com.br>
Reis
Bernardo Sgarbi
BS
Fernandes
Viviane Cristina
VC
Costa
Míriam Maria Silva
MM
Goes
Alfredo Miranda
AM
de Andrade
Antero Silva Ribeiro
AS
D000818:Animals; D005657:Fungal Vaccines / Q000276:immunology* / Q000494:pharmacology; D005720:Gamma Rays; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology; D015848:Interleukin-5 / Q000276:immunology; D007843:Latin America; D051379:Mice; D008807:Mice, Inbred BALB C; D010228:Paracoccidioides / Q000276:immunology* / Q000472:pathogenicity; D010229:Paracoccidioidomycosis / Q000276:immunology / Q000517:prevention & control*; D018417:Th1 Cells / Q000276:immunology; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000276:immunology / Q000494:pharmacology
D005657:Fungal Vaccines; D007074:Immunoglobulin G; D015848:Interleukin-5; D014613:Vaccines, Attenuated; D016753:Interleukin-10; D007371:Interferon-gamma