title: Protein expression changed by nicotine in rat vascular smooth muscle cells.
abstract: In order to observe the effects of nicotine on protein expression in rat vascular smooth muscle cells (SMCs), nicotine treated SMCs were studied by proteomic technologies combining two-dimensional electrophoresis (2-DE) and peptide mass fingerprinting (PMF). Real-time RT-PCR was used to validate the differentially expressed proteins. We found that 11 protein spots were significantly up-regulated and one down-regulated by nicotine treatment. The results of PMF showed that these up- and down-regulated proteins could be divided into three groups according to their functions: cytoskeleton proteins, regulatory proteins and enzymes. Simultaneously, we also verified their consistent alteration at the transcriptional level through real-time RT-PCR. The affected proteins turned out to be mainly associated with cell migration, proliferation and energy metabolism, and are responsible for nicotine-related cardiovascular damage.
authors.affiliation: School of Life Science and Technology, Shanghai Jiao Tong University, Shanghai, PR China.
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: W
authors.initials: W
authors.lastname: Wu
authors.forename: X
authors.initials: X
authors.lastname: Fang
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Xiao
authors.forename: R
authors.initials: R
authors.lastname: Zhao
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: Z
authors.initials: Z
authors.lastname: Qiao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D007150:Immunohistochemistry; D008297:Male; D013058:Mass Spectrometry; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009538:Nicotine / Q000494:pharmacology*; D010449:Peptide Mapping; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014357:Trypsin / Q000494:pharmacology
chemical_list: D011506:Proteins; D020543:Proteome; D009538:Nicotine; D014357:Trypsin