title: Production of L2 lipase by Bacillus sp. strain L2: nutritional and physical factors.
abstract: A thermophilic bacterium, Bacillus sp. strain L2 was isolated from a hot spring in Perak, Malaysia. An extracellular lipase activity was detected through plate and broth assays at 70 degrees C after 28 h of incubation. The L2 lipase production was growth dependent as revealed by a number of factors affecting the secretion of extracelullar lipase. As for nutritional factors, casamino acids, trehalose, Ca(2+) and Tween 60 were found to be more effective for lipase production. The optimum physical condition for L2 lipase production was obtained at 70 degrees C after 28 h of cultivation time, at pH 7.0, 150 rpm of agitation rate and 1% of starting inoculum size. The activity staining of crude L2 lipase revealed a clearing zone at 39 kDa.
authors.affiliation: Enzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Fairolniza Mohd
authors.initials: FM
authors.lastname: Shariff
authors.forename: Thean Chor
authors.initials: TC
authors.lastname: Leow
authors.forename: A D
authors.initials: AD
authors.lastname: Mukred
authors.forename: Abu Bakar
authors.initials: AB
authors.lastname: Salleh
authors.forename: Mahiran
authors.initials: M
authors.lastname: Basri
authors.forename: Raja Noor Zaliha Raja Abd
authors.initials: RN
authors.lastname: Rahman
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D045482:Hot Springs / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D008049:Lipase / Q000096:biosynthesis* / Q000302:isolation & purification; D008296:Malaysia; D013696:Temperature
chemical_list: D008049:Lipase