Immobilization staphylococcal protein a on magnetic cellulose microspheres for IgG affinity purification.
The main objective of this study is to develop and evaluate the immobilization of Staphylococcal Protein A on magnetic cellulose microspheres (SPA-MCMS) for immunological capture of IgG. After cloning, expression and separation, SPA was immobilized onto MCMS to prepare a magnetic affinity media subject to the purification of IgGs. The binding capacity, binding time, leakage of SPA and its reproducibility were optimized to improve the binding efficiency with an appropriate amount and recovery of IgG. Rabbit IgG was successfully purified from serum in a single-step by SPA-MCMS with an overall recovery of 73.18% and purity of 90.27%. Therefore, this study effectively illustrated the advantages of magnetic microcarriers for rapid and efficient purification of antibodies. The separation media shows a high potential for the future development of affinity isolation and immunodiagnostic application.
Cao
Yu
Y
Department of Microbiology, College of Life Sciences, Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin, China.
Tian
Wang
W
Gao
Shengyan
S
Yu
Yangsheng
Y
Yang
Wenbo
W
Bai
Gang
G
D007074:Immunoglobulin G / Q000302:isolation & purification*; D007218:Industrial Microbiology; D008280:Magnetics; D008863:Microspheres; D013205:Staphylococcal Protein A / Q000276:immunology
D007074:Immunoglobulin G; D013205:Staphylococcal Protein A