[Overexpression of the Cdc42 in the brain tissue of human with intractable temporal epilepsy].
OBJECTIVE
To investigate the expression of cell division cycle 42 GTP-binding protein (Cdc42) in the brain tissue of patients with intractable temporal epilepsy and discuss its effect on epileptogenesis.


METHODS
Twenty samples brain tissues were collected from the brain tissue bank of intractable temporal epilepsy established by Epilepsy Preventive and Therapeutic Center of Chongqing. The expression of Cdc42 was determined by immunohistochemistry and fluoroimmunoassay. Twenty brain tissue samples were collected from autopsy on 20 sex and age-matched persons who died from accident were used as controls.


RESULTS
The absorption values of Cdc42 in the temporal lobe of the patients was 0.038 +/- 0.016, significantly higher than that of the controls (0.020 +/- 0.005, t = 14.59, P < 0.01). The absorption values of Cdc42 in the hippocampus of the patients was 0.049 +/- 0.009, t = 12.01, P < 0.01). Fluoroimmunoassay showed that the fluorescence intensity of Cdc42 in the temporal lobe of the patients was 18.3 +/- 2.5, significantly higher than that of the controls (11.8 +/- 2.9, t = 33.77, P < 0.01), and the Fluorescence intensity of Cdc42 in the hippocampus of the patients was and control hippocampus were 23.8 +/- 3.3, significantly higher than that of the controls (10.1 +/- 2.6, t = 12.01, P < 0.01).


CONCLUSIONS
The overexpression of Cdc42 in the cortex of temporal lobe and hippocampus may play an important role in the epileptogenic mechanisms of intractable epilepsy.
Xiao
Fei
F
Department of Neurology, First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Chongqing 400016, China.
He
Mei
M
Wang
Xue-feng
XF
Xi
Zhi-qin
ZQ
Li
Jin-mei
JM
Wu
Yuan
Y
Zhong
Zheng-fu
ZF
Zhu
Dan
D
Lu
Yong
Y
Li
Hong-wei
HW
D000293:Adolescent; D000328:Adult; D001344:Autopsy; D001921:Brain / Q000378:metabolism / Q000473:pathology*; D001923:Brain Chemistry; D004827:Epilepsy / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015200:Fluoroimmunoassay; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D013702:Temporal Lobe / Q000378:metabolism / Q000473:pathology; D055815:Young Adult; D020764:cdc42 GTP-Binding Protein / Q000096:biosynthesis*
D020764:cdc42 GTP-Binding Protein