Immunogenicity of novel recombinant human activated factor VII analogues on factor VII neonatally-tolerized rats.
Recombinant activated factor VII (rFVIIa; NovoSeven) has been widely used to treat bleeding in patients with haemophilia with inhibitors. To increase the intrinsic activity, analogues of rFVIIa (rFVIIa Q, rFVIIa DVQ, and rFVIIa DVQA) with altered amino acid sequence at or near the active centre have been developed. The immunogenicity of these analogues was tested in a rat immune tolerance model. Neonatal rats received rFVIIa intraperitoneally on post-natal Day 1 and were subsequently challenged with rFVIIa in Freunds Incomplete Adjuvant subcutaneously on Days 10 and 24. Rats were tested for tolerance on Day 32; the tolerant cohort and a parallel cohort of untreated control rats were challenged with rFVIIa, rFVIIa Q, rFVIIa DVQ, or rFVIIa DVQA on Days 46 and 76. Immune responses determined by enzymelinked immunosorbent assay (ELISA) on Day 84 showed no statistically significant difference between the responses in the four control cohorts. Immune responses were higher in the control than in the tolerant cohort. Compared with rFVIIa (4/16), there was no difference in the proportion of rats that broke tolerance following challenge with rFVIIa DVQ (3/16) and rFVIIa DVQA (7/16), whereas a statistically significant greater proportion broke tolerance after challenge with rFVIIa Q (11/16). Therefore, in this model rFVIIa DVQ or rFVIIa DVQA were not more immunogenic than rFVIIa.
Sommer
Christian
C
Biopharm Toxicology and Safety Pharmacology, Novo Nordisk A/S, Måløv, Denmark. csom@novonordisk.com
Norbert Jørgensen
Peer
P
Salanti
Zaki
Z
Clausen
Jes Thorn
JT
Jensen
Lisbeth Bjerring
LB
D000818:Animals; D000831:Animals, Newborn; D001665:Binding Sites; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay; D015942:Factor VIIa / Q000737:chemistry* / Q000276:immunology; D005620:Freund's Adjuvant / Q000737:chemistry; D006801:Humans; D007107:Immune System; D007108:Immune Tolerance; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D013927:Thrombosis
D011994:Recombinant Proteins; D005620:Freund's Adjuvant; C103587:recombinant FVIIa; D015942:Factor VIIa