title: Polymerase chain reaction identification of three members of the Anopheles sundaicus (Diptera: Culicidae) complex, malaria vectors in Southeast Asia.
abstract: Anopheles sundaicus s.l., a major malaria vector taxon, occurs primarily along coastal areas and on islands in Southeast Asia. Our previous studies using cytochrome oxidase I, cytochrome-b, and internal transcribed spacer 2 markers discriminated three allopatric species: An. sundaicus s.s. in northern Borneo, An. epiroticus in Southeast Asia, and An. sundaicus E on Sumatra and Java, Indonesia. Morphological comparisons of three developmental stages did not reveal unique diagnostic characters that could reliably distinguish the three species. Therefore, we developed a multiplex polymerase chain reaction (PCR) assay based on two mitochondrial DNA markers to unambiguously identify them. This PCR was tested on 374 specimens from 24 different geographical populations, expanding our knowledge of the distribution of these species.
authors.affiliation: Institut de Recherche pour le Développement, UMR22 Biologic et Gestion des Populations, Campus de Baillarguet, CS30016, Montferrier sur Lez 34988, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Dusfour
authors.forename: Johanna
authors.initials: J
authors.lastname: Blondeau
authors.forename: Ralph E
authors.initials: RE
authors.lastname: Harbach
authors.forename: Indra
authors.initials: I
authors.lastname: Vythilingham
authors.forename: Visut
authors.initials: V
authors.lastname: Baimai
authors.forename: Ho D
authors.initials: HD
authors.lastname: Trung
authors.forename: Tho
authors.initials: T
authors.lastname: Sochanta
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bangs
authors.forename: Sylvie
authors.initials: S
authors.lastname: Manguin
mesh_terms: D000818:Animals; D000852:Anopheles / Q000033:anatomy & histology / Q000235:genetics*; D001210:Asia, Southeastern; D001483:Base Sequence; D045303:Cytochromes b / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005260:Female; D007303:Insect Vectors / Q000033:anatomy & histology / Q000235:genetics*; D008288:Malaria / Q000635:transmission*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D013045:Species Specificity
chemical_list: D045303:Cytochromes b; D003576:Electron Transport Complex IV