A novel PCR-RFLP assay for the detection of the single nucleotide polymorphism at position +1440 in the human CXCR2 gene.
We designed a novel PCR-RFLP assay to genotype for the CXCR2 +1440 (G/A) single nucleotide polymorphism, which provides a simple, low-cost, practical, and reproducible method. Allele frequencies in healthy Brazilian individuals were found to be 0.65% for allele A and 0.35% for allele G.
Viana
Aline C
AC
Department of Diagnosis and Surgery, School of Dentistry, UNESP - São Paulo State University, R. Humaitá, 1680 Centro, Araraquara, Sao Paulo, 14801-903, Brazil.
Kim
Yeon J
YJ
Cirelli
Joni A
JA
Orrico
Silvana R P
SR
Curtis
Karen C
KC
Cano
Veridiana S P
VS
Valentini
Sandro R
SR
Scarel-Caminaga
Raquel Mantuaneli
RM
D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods; D001483:Base Sequence; D001938:Brazil; D016022:Case-Control Studies; D017931:DNA Primers / Q000235:genetics; D005787:Gene Frequency; D006801:Humans; D010510:Periodontal Diseases / Q000235:genetics / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D023063:Receptors, Interleukin-8B / Q000235:genetics*
D017931:DNA Primers; D023063:Receptors, Interleukin-8B