title: Prevalence of human papillomavirus types 6, 11, 16, 18, 31, and 33 in a cohort of Greek women.
abstract: To study HPV prevalence and HPV types 6, 11, 16, 18, 31, and 33 distribution in cervical smears in a cohort of Greek women. One thousand six hundred thirty-six samples were cytologically evaluated and molecularly analyzed, by PCR based assay. Abnormal cytology was identified in 997 women and 75.4% of them were HPV DNA positive, while 639 had normal cytology and 24.6% were HPV DNA positive. HPV was detected in 62.9% of 256 ASCUS smears, 89.3% of 516 LSIL, 86.7% of 60 HSIL and 47.3% of 165 with cervical carcinoma. Overall, HPV 11 was the most common type (13.4%), followed by 18 (10.3%), 6 (7.2%), 16 (6.4%), 31 (3.4%) and 33 (3.4%). Multiple infections with two (11.3%) or more types, primarily 11 and 18 (4.8%), were also identified. Low-risk types 11 and 6 were common in ASCUS (36.6% and 26.4%, respectively), and high-risk types 16 and 18 in HSIL (42.3% and 30.8%, respectively) and in cancer (51.3% and 41%, respectively). Multiple infections were detected in 2.2% of normal and 31.7% of HSIL. HPV prevalence was 75.4% in abnormal and 24.6% in normal cervical smears. HPV 16 and 18 were the most common types in cancer. Single infection with type 11 and multiple infections with 11 and 18 were more frequent.
authors.affiliation: Department of Virology, Papanicolaou Research Centre of Oncology and Experimental Surgery, Regional Anticancer Oncology Hospital of Athens St. Savvas, Athens, Greece. iolpanot@agsavvas-hosp.gr
authors.forename: Efstathia
authors.initials: E
authors.lastname: Panotopoulou
authors.forename: Aliki
authors.initials: A
authors.lastname: Tserkezoglou
authors.forename: Maria
authors.initials: M
authors.lastname: Kouvousi
authors.forename: Ioanna
authors.initials: I
authors.lastname: Tsiaousi
authors.forename: George
authors.initials: G
authors.lastname: Chatzieleftheriou
authors.forename: Demitra
authors.initials: D
authors.lastname: Daskalopoulou
authors.forename: George
authors.initials: G
authors.lastname: Magiakos
mesh_terms: D000293:Adolescent; D000328:Adult; D052685:Alphapapillomavirus / Q000145:classification*; D015331:Cohort Studies; D005260:Female; D006115:Greece / Q000453:epidemiology; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D015995:Prevalence; D002577:Uterine Cervical Diseases / Q000453:epidemiology* / Q000821:virology*