Fervidobacterium changbaicum sp. nov., a novel thermophilic anaerobic bacterium isolated from a hot spring of the Changbai Mountains, China.
A thermophilic, obligately anaerobic, rod-shaped bacterium (strain CBS-1(T)) was isolated from a hot spring mixture of water and mud of the Changbai Mountains, China. Strain CBS-1(T) was found to be non-sporulating, Gram-negative, with optimal growth at 75-80 degrees C. It grew on a wide range of carbon sources, including glucose, lactose, maltose, starch, sorbitol and pyruvate amongst others. The DNA G+C content of strain CBS-1(T) was 31.9 mol%. The 16S rRNA gene sequence analysis indicated that the strain was a member of the genus Fervidobacterium. The high concentration of C(16 : 0) (52.2 %) in the fatty acid profile of the cell envelope supported its inclusion as a member of the genus Fervidobacterium. On the basis of the low values of DNA-DNA hybridization (25.8 and 20.5 %) and phenotypic features, strain CBS-1(T) represents a novel species of the genus Fervidobacterium, for which the name Fervidobacterium changbaicum sp. nov. is proposed. The type strain is CBS-1(T) (=DSM 17883(T)=JCM 13353(T)).
Cai
Jingang
J
Key Laboratory for Molecular Enzymology and Engineering, Jilin University, Changchun 130023, PR China.
Wang
Yanping
Y
Key Laboratory for Molecular Enzymology and Engineering, Jilin University, Changchun 130023, PR China.
Liu
Dongbo
D
School of Life Science, Northeast Normal University, Changchun 130024, PR China.
Zeng
Yan
Y
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
Xue
Yanfen
Y
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
Ma
Yanhe
Y
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
Feng
Yan
Y
Key Laboratory for Molecular Enzymology and Engineering, Jilin University, Changchun 130023, PR China.
D001421:Bacteria, Anaerobic / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002244:Carbon / Q000378:metabolism; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013171:Spores, Bacterial / Q000166:cytology; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002244:Carbon