title: Optimization of environmental parameters for biodegradation of alpha and beta endosulfan in soil slurry by Pseudomonas aeruginosa.
abstract: OBJECTIVE
  To determine optimal environmental conditions for achieving biodegradation of alpha- and beta-endosulfan in soil slurries following inoculation with an endosulfan degrading strain of Pseudomonas aeruginosa.
  
  
  RESULTS
  Parameters that were investigated included soil texture, soil slurry: water ratios, initial inoculum size, pH, incubation temperature, aeration, and the use of exogenous sources of organic and amino acids. The results showed that endosulfan degradation was most effectively achieved at an initial inoculum size of 600 microl (OD = 0 x 86), incubation temperature of 30 degrees C, in aerated slurries at pH 8, in loam soil. Under these conditions, the bacterium removed more than 85% of spiked alpha- and beta-endosulfan (100 mg l(-1)) after 16 days. Abiotic degradation in noninoculated control medium within same incubation period was about 16%. Biodegradation of endosulfan varied in different textured soils, being more rapid in course textured soil than in fine textured soil. Increasing the soil contents in the slurry above 15% resulted in less biodegradation of endosulfan. Exogenous application of organic acids (citric acid and acetic acid) and amino acids (L-methionine and L-cystein) had stimulatory and inhibitory effects, respectively, on biodegradation of endosulfan.
  
  
  CONCLUSIONS
  The results of this study demonstrated that biodegradation of endosulfan by Ps. aeruginosa in soil sediments enhanced significantly under optimized environmental conditions.
  
  
  CONCLUSIONS
  Endosulfan is a commonly used pesticide that can contaminate soil, wetlands and groundwater. Our study demonstrates that bioaugmentation of contaminated soils with an endosulfan degrading bacterium under optimized conditions provides an effective bioremediation strategy.
authors.affiliation: Institute of Soil & Environmental Sciences, University of Agriculture, Faisalabad, Pakistan. bio@fsd.comsats.net.pk
authors.forename: M
authors.initials: M
authors.lastname: Arshad
authors.forename: S
authors.initials: S
authors.lastname: Hussain
authors.forename: M
authors.initials: M
authors.lastname: Saleem
mesh_terms: D019342:Acetic Acid; D000596:Amino Acids / Q000378:metabolism; D001673:Biodegradation, Environmental; D019343:Citric Acid; D004726:Endosulfan / Q000032:analysis / Q000633:toxicity*; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000633:toxicity*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000633:toxicity*; D013696:Temperature
chemical_list: D000596:Amino Acids; D007306:Insecticides; D012987:Soil; D012989:Soil Pollutants; D019343:Citric Acid; D004726:Endosulfan; D019342:Acetic Acid