FBXO40, a gene encoding a novel muscle-specific F-box protein, is upregulated in denervation-related muscle atrophy.
F-box proteins are key components of SCF (Skp1-Cullin1-F-box protein) complexes, which exert E3 ubiquitin ligase activity and participate in cell cycle and signal transduction. F-box proteins interact with Skp1 through the F-box domain and with proteins to be ubiquitinated through other interaction domains. We have characterized a novel muscle-specific F-box protein, FBXO40, the expression of which decreases in the dystrophic muscle of Limb-girdle muscular dystrophy (LGMD) patient. During the development of skeletal muscle, FBXO40 can only be detected at postnatal stage from about 2 weeks after birth. By overexpressing in C2C12 cells, FBXO40 localized in cytoplasm. Most importantly, the expression of FBXO40 can be upregulated in skeletal muscle from denervation- but not starvation-related muscle atrophy. All our data suggest that FBXO40 may function as a regulator involved in the postnatal myogenesis.
Ye
Jianwei
J
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, PR China.
Zhang
Yong
Y
Xu
Jialin
J
Zhang
Qiang
Q
Zhu
Dahai
D
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D044783:F-Box Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009121:Muscle Denervation; D024510:Muscle Development / Q000235:genetics; D009124:Muscle Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D018482:Muscle, Skeletal / Q000254:growth & development / Q000294:innervation; D009133:Muscular Atrophy / Q000235:genetics*; D049288:Muscular Dystrophies, Limb-Girdle / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation
D044783:F-Box Proteins; C525752:FBXO40 protein, human; C525753:FBXO40 protein, mouse; C525754:FBXO40 protein, rat; D009124:Muscle Proteins; D012333:RNA, Messenger