Cyclin G associated kinase interacts with interleukin 12 receptor beta2 and suppresses interleukin 12 induced IFN-gamma production.
Interleukin 12 receptor beta1 (IL-12Rbeta1) and beta2 (IL-12Rbeta2) constitute the functional and high-affinity receptor complex for interleukin 12 (IL-12) and mediate important functions in activated T cells. In this study, we identified cyclin G associated kinase (GAK) as a new IL-12Rbeta2-interacting protein using yeast two-hybrid system and confirmed it by coimmunoprecipitation assays. Overexpression of GAK in activated T cells suppresses IL-12 induced IFN-gamma production but has no detectable effects on its proliferation, whereas knockdown of GAK by RNA interference (RNAi) increases IFN-gamma production. These results suggest that GAK associates with IL-12Rbeta2 and may play a role in regulating IL-12 signaling.
Lin
Ying
Y
Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Tang
Yu Jie
YJ
Zong
Hong Liang
HL
Gu
Jian Xin
JX
Deng
Wei Wen
WW
Wang
Chen
C
Sun
Bing
B
D000818:Animals; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism*; D018664:Interleukin-12 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D053712:Interleukin-12 Receptor beta 2 Subunit / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D034741:RNA, Small Interfering / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*; D020798:Two-Hybrid System Techniques
C534703:Ccng1 protein, mouse; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins; D053712:Interleukin-12 Receptor beta 2 Subunit; D034741:RNA, Small Interfering; D018664:Interleukin-12; D007371:Interferon-gamma