Electrospray ionization and time-of-flight mass spectrometric method for simultaneous determination of spermidine and spermine.
A sensitive method for the determination of polyamines in mammalian cells was described using electrospray ionization and time-of-flight mass spectrometer. This method was 50-fold more sensitive than the previous method using ionspray ionization and quadrupole mass spectrometer. The method employed the partial purification and derivatization of polyamines, but allowed a measurement of multiple samples which contained picomol amounts of polyamines. Time required for data acquisition of one sample was approximately 2 min. The method was successfully applied for the determination of reduced spermidine and spermine contents in cultured cells under the inhibition of aminopropyltransferases. In addition, a new proper internal standard was proposed for the tracer experiment using (15)N-labeled polyamines.
Samejima
Keijiro
K
Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo, Japan. samejima@musashino-u.ac.jp
Otani
Masahiro
M
Murakami
Yasuko
Y
Oka
Takami
T
Kasai
Misao
M
Tsumoto
Hiroki
H
Kohda
Kohfuku
K
D002460:Cell Line; D002477:Cells / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003514:Cyclohexylamines / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D013058:Mass Spectrometry; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization; D013095:Spermidine / Q000032:analysis*; D013096:Spermine / Q000032:analysis*
D003514:Cyclohexylamines; D004791:Enzyme Inhibitors; D011506:Proteins; D013096:Spermine; C059116:4-methylcyclohexylamine; D013095:Spermidine