Hypermethylation of p16INK4a in Korean non-small cell lung cancer patients.
Promoter hypermethylation of the p16(INK4a) gene was investigated in 81 sets of samples of tumor tissue and adjacent normal tissue from Korean patients with primary lung cancer, using the modified real-time polymerase chain reaction (PCR)/ SYBR Green detection method. The results showed hypermethylation of p16(INK4a) in 27.2% of tumor tissues, and in 11.1% of adjacent normal tissue. No significant association was found between the overall aberrant methylation in tumor and corresponding normal specimens (r=0.137, p=0.219). In 22 cases with p16(INK4a) hypermethylation in tumor tissues, only 4 (18.1%) cases were found to have a hypermethylated normal tissue specimen. The findings of this study show that smoking can influence the methylation level of the promoter region of p16(INK4a), and that this occurs in tumor tissues more frequently than in normal tissues. Other clinicopathological characteristics, including age, sex, tumor stage, and histologic type were not found to be correlated with p16(INK4a) methylation.
Hong
Young Seoub
YS
Department of Preventive Medicine, College of Medicine, Dong-A University, Busan, Korea. yshong@dau.ac.kr
Roh
Mee Sook
MS
Kim
Na Young
NY
Lee
Hye Jung
HJ
Kim
Hee Kyoung
HK
Lee
Kyung Eun
KE
Kwak
Jong Young
JY
Kim
Joon Youn
JY
D000328:Adult; D000368:Aged; D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D019175:DNA Methylation; D017931:DNA Primers / Q000235:genetics; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D005260:Female; D019942:Genes, p16; D006801:Humans; D007723:Korea; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012907:Smoking / Q000009:adverse effects / Q000235:genetics
D017931:DNA Primers; D004273:DNA, Neoplasm