title: Immunological and microbiological activity of Davilla elliptica St. Hill. (Dilleniaceae) against Mycobacterium tuberculosis.
abstract: Mycobacterium tuberculosis is responsible for over 8 million cases of tuberculosis (TB) annually. Natural products may play important roles in the chemotherapy of TB. The immunological activity of Davilla elliptica chloroform extract (DECE) was evaluated in vitro by the determination of hydrogen peroxide (H2O2), nitric oxide (NO), and tumor necrosis factor-alpha (TNF-alpha) release in peritoneal macrophages cultures. DECE was also tested for its antimycobacterial activity against M. tuberculosis using the microplate alamar blue assay. DECE (50, 150, 250 microg/ml) stimulated the production of H2O2 (from 1,79 +/- 0,23 to 7,27 +/- 2,54; 15,02 +/- 2,86; 20,5 +/- 2,1 nmols) (means +/- SD), NO (from 2,64 +/- 1,02 to 25,59 +/- 2,29; 26,68 +/- 2,41; 29,45 +/- 5,87 micromols) (means +/- SD) and TNF-alpha (from 2,44 +/- 1,46 to 30,37 +/- 8,13; 38,68 +/- 1,59; 41,6 +/- 0,90 units/ml) (means +/- SD) in a dose-dependent manner and also showed a promising antimycobacterial activity with a minimum inhibitory concentration of 62,5 microg/ml. This plant may have therapeutic potential in the immunological and microbiological control of TB.
authors.affiliation: Departamento de Análises Clínicas, Unesp, R. Expedicionários do Brasil 1601, 14801-902 Araraquara, SP, Brasil.
authors.forename: Flávia Cristine
authors.initials: FC
authors.lastname: Mascia Lopes
authors.forename: Marisa Campos
authors.initials: MC
authors.lastname: Polesi Placeres
authors.forename: Cleso Mendonça
authors.initials: CM
authors.lastname: Jordão
authors.forename: Célio Takashi
authors.initials: CT
authors.lastname: Higuchi
authors.forename: Daniel
authors.initials: D
authors.lastname: Rinaldo
authors.forename: Wagner
authors.initials: W
authors.lastname: Vilegas
authors.forename: Clarice Queico
authors.initials: CQ
authors.lastname: Fujimura Leite
authors.forename: Iracilda Zeppone
authors.initials: IZ
authors.lastname: Carlos
mesh_terms: D000818:Animals; D000904:Antibiotics, Antitubercular / Q000302:isolation & purification / Q000494:pharmacology*; D045466:Dilleniaceae / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D009569:Nitric Oxide / Q000096:biosynthesis; D010936:Plant Extracts / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000904:Antibiotics, Antitubercular; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D006861:Hydrogen Peroxide