title: Cell attachment to PET films coated with a thermo-sensitive block co-polymer with different chemical composition.
abstract: This objective of this study is to characterize the surface of poly(ethylene terephthalate) (PET) films coated with the thermo-sensitive di-block co-polymers of 2-ethoxyethyl vinyl ether and 2-phenoxyethyl vinyl ether segments (EOVE-b-PhOVE) with a high polydispersity and evaluate the behavior of cell attachment on them at different temperatures. The EOVE segment possessed a low critical solution temperature at 20 degrees C while the hydrophobic PhOVE segment functioned as the site to allow the co-polymer to adsorb onto the PET films. X-ray photoelectron spectroscopy and contact angle measurements revealed that the PET film was coated with the EOVE-b-PhOVE co-polymers. The density of co-polymers coated increased with the concentration of co-polymers used for coating. Irrespective of the co-polymer type, 3T3L1 cells attached on the surface of coated films at 37 degrees C, while the cells showed a spread shape, which is similar to that of cells attached on the original non-coated film. However, when the temperature decreased from 37 to 4 degrees C, the cell shape changed to be round, in contrast to that of the original PET film. The percent increase of round cells depended on the coating density and the polymerization degree of EOVE segment.
authors.affiliation: Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Takamoto
authors.forename: Kaori
authors.initials: K
authors.lastname: Yasuda
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Tsujino
authors.forename: Shinji
authors.initials: S
authors.lastname: Sugihara
authors.forename: Shokyoku
authors.initials: S
authors.lastname: Kanaoka
authors.forename: Sadahito
authors.initials: S
authors.lastname: Aoshima
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D048430:Cell Shape; D051379:Mice; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D013696:Temperature
chemical_list: D011093:Polyethylene Terephthalates