[Surveillance on Rickettsia in epidemic areas of scrub typhus in Xinyang areas of Henan].
OBJECTIVE
To understand the epidemic status of Rickettsia in Xinyang areas of Henan province.


METHODS
Samples including liver, spleen, kidney from mouse and chigger mites from Xinyang areas and serum samples were detected by nested-polymerase chain reaction (PCR) and indirect immunofluorescence assay (IFA).


RESULTS
In 62 viscus samples from mice organs, the positive rates were 16.13%, 8.06% and 6.45% for Orientia tsutsugamushi, R. typhii and Spotted fever group rickettsiae respectively. In blood clots samples from mice, the positive rates were 8.06%, 6.45% and 1.61 % for O. tsutsugamushi, R. typhii and Spotted fever group rickettsiae respectively. Three out of 26 mouse serum samples were positive for the predicted fluorexcent intensity O. tsutsugamushi.


CONCLUSIONS
Using nested-PCR and IFA methods, O. tsutsugamushi, R. typhii and Spotted fever group rickettsiae were detected in the captured mice living in Xinyang areas of Henan province. Results showed that there were intensive natural reserviors of Rickettsia in Henan province, suggesting that the risk of outbreak of Rickettsia in these areas was high.
Fu
Xiu-ping
XP
Rickettsia Disease Department, National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Zhang
Jing-shan
JS
Shen
Xiao-jing
XJ
Luan
Ming-chun
MC
Li
Meng-lei
ML
Zhang
Li-juan
LJ
D000818:Animals; D002681:China; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007668:Kidney / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D051379:Mice; D012285:Orientia tsutsugamushi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012281:Rickettsia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D012612:Scrub Typhus / Q000453:epidemiology* / Q000382:microbiology*; D013154:Spleen / Q000382:microbiology