title: Cell-free protein synthesis in microfluidic array devices.
abstract: We report the development of a microfluidic array device for continuous-exchange, cell-free protein synthesis. The advantages of protein expression in the microfluidic array include (1) the potential to achieve high-throughput protein expression, matching the throughput of gene discovery; (2) more than 2 orders of magnitude reduction in reagent consumption, decreasing the cost of protein synthesis; and (3) the possibility to integrate with detection for rapid protein analysis, eliminating the need to harvest proteins. The device consists of an array of units, and each unit can be used for production of an individual protein. The unit comprises a tray chamber for in vitro protein expression and a well chamber as a nutrient reservoir. The tray is nested in the well, and they are separated by a dialysis membrane and connected through a microfluidic connection that provides a means to supply nutrients and remove the reaction byproducts. The device is demonstrated by synthesis of green fluorescent protein, chloramphenicol acetyl-transferase, and luciferase. Protein expression in the device lasts 5-10 times longer and the production yield is 13-22 times higher than in a microcentrifuge tube. In addition, we studied the effects of the operation temperature and hydrostatic flow on the protein production yield.
authors.affiliation: Department of Mechanical and Aerospace Engineering, University of Florida, PO Box 116250, Gainesville, Florida 32611, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Mei
authors.forename: Carl K
authors.initials: CK
authors.lastname: Fredrickson
authors.forename: Andrew
authors.initials: A
authors.lastname: Simon
authors.forename: Ruba
authors.initials: R
authors.lastname: Khnouf
authors.forename: Z Hugh
authors.initials: ZH
authors.lastname: Fan
mesh_terms: D002474:Cell-Free System; D004867:Equipment Design; D008422:Materials Testing; D046210:Microfluidic Analytical Techniques; D014176:Protein Biosynthesis; D013696:Temperature