title: Impact of dialyzer membrane on apoptosis and function of polymorphonuclear cells and cytokine synthesis by peripheral blood mononuclear cells in hemodialysis patients.
abstract: In an in vivo crossover trial, we compared a cellulosic with a synthetic dialyzer with respect to polymorphonuclear cells (PMN) function and apoptosis, cytokine serum levels and synthesis by peripheral blood mononuclear cells (PBMC), and complement activation. Twenty hemodialysis (HD) patients were assigned in alternate order to HD with cellulose acetate (CA) or polysulfone (PS) dialyzer. After 2 weeks, patients were crossed over to the second dialyzer and treated for another 2 weeks. Apoptosis was assessed by flow cytometry in freshly isolated PMN. Phagocytosis and production of peroxide by PMN were studied by flow cytometry in whole blood. PBMC were isolated from blood samples and incubated for 24 h with or without lipopolysaccharide (LPS). There was no impact of dialyzer biocompatibility on PMN apoptosis and function, cytokine synthesis by PBMC or on their serum levels, serum levels of C3a, and terminal complement complex (TCC). Nevertheless, after HD, serum levels of complement correlated negatively with PMN phagocytosis and peroxide production, and positively with PMN apoptosis and cytokine production by PBMC. Although the results did not show a dialyzer advantage on the immunologic parameters, complement activation may have modulated cell function and apoptosis after HD.
authors.affiliation: Department of Medicine, Division of Nephrology, Federal University of Sao Paulo-UNIFESP, Saõ Paulo, SP, Brazil. mclaudia@einstein.br
authors.forename: Maria C C
authors.initials: MC
authors.lastname: Andreoli
authors.forename: Maria A
authors.initials: MA
authors.lastname: Dalboni
authors.forename: Renato
authors.initials: R
authors.lastname: Watanabe
authors.forename: Silvia R
authors.initials: SR
authors.lastname: Manfredi
authors.forename: Maria E F
authors.initials: ME
authors.lastname: Canziani
authors.forename: Esper G
authors.initials: EG
authors.lastname: Kallás
authors.forename: Ricardo C
authors.initials: RC
authors.lastname: Sesso
authors.forename: Sergio A
authors.initials: SA
authors.lastname: Draibe
authors.forename: Vaidyanathapuram S
authors.initials: VS
authors.lastname: Balakrishnan
authors.forename: Bertrand L
authors.initials: BL
authors.lastname: Jaber
authors.forename: Orfeas
authors.initials: O
authors.lastname: Liangos
authors.forename: Miguel
authors.initials: M
authors.lastname: Cendoroglo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000187:drug effects*; D001672:Biocompatible Materials / Q000494:pharmacology*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis; D006801:Humans; D008567:Membranes, Artificial; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D011108:Polymers / Q000494:pharmacology*; D006435:Renal Dialysis / Q000295:instrumentation; D013450:Sulfones / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; C017662:polysulfone P 1700; C005062:acetylcellulose; D002482:Cellulose