[Determination of alpha-glucosidase activity in seminal plasma by semi-automatic biochemistry analyzer].
OBJECTIVE
To establish a method of determining alpha-glucosidase activity in seminal plasma by semi-automatic biochemistry analyzer.


METHODS
Alpha-glucosidase activity in seminal plasma from 51 men with normal semen parameters in routine semen analysis were detected by semi-automatic biochemistry analyzer and manual glucose oxidase method, respectively. Then, the intra-assay and inter-assay coefficient variation (CV) and normal reference value were calculated. In the meanwhile, the correlation between the two methods was analyzed.


RESULTS
The intra-assay CVs of 2 seminal plasma samples with different alpha-glucosidase activity detected by semi-automatic biochemistry analyzer were 12.63% and 9.13%, and the inter-assay CVs were 10.67% and 13.49%, respectively. The normal reference value for seminal alpha-glucosidase activity detected with semi-automatic biochemistry analyzer ranged from 102.28 to 555.08 U/L. There was a significantly positive correlation between the semi-automatic biochemistry analyzer and the manual glucose oxidase method (r = 0.792, P < 0.01).


CONCLUSIONS
The method of determining alpha-glucosidase activity in seminal plasma by semi-automatic biochemistry analyzer, with its simplicity, less cost of time and reagents, and more reliable result, could be applied to clinical laboratory medicine.
Lu
Jin-chun
JC
Department of Reproduction & Genetics, PLA Institute of Clinical Laboratory Medicine, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China. jclu@jlonline.com
Xu
Hui-ru
HR
Huang
Yu-feng
YF
D000328:Adult; D001671:Biochemistry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008297:Male; D012016:Reference Values; D015203:Reproducibility of Results; D012661:Semen / Q000201:enzymology*; D000520:alpha-Glucosidases / Q000032:analysis / Q000378:metabolism*
D000520:alpha-Glucosidases