Expression of neuropeptide W in rat stomach mucosa: regulation by nutritional status, glucocorticoids and thyroid hormones.
Neuropeptide W (NPW) is a recently identified neuropeptide that binds to G-protein-coupled receptor 7 (GPR7) and 8 (GPR8). In rodent brain, NPW mRNA is confined to specific nuclei in hypothalamus, midbrain and brainstem. Expression of NPW mRNA has also been confirmed in peripheral organs such as stomach. Several reports suggested that brain NPW is implicated in the regulation of energy and hormonal homeostasis, namely the adrenal and thyroid axes; however the precise physiological role and regulation of peripheral NPW remains unclear. In this study, we examined the effects of nutritional status on the regulation of NPW in stomach mucosa. Our results show that in this tissue, NPW mRNA and protein expression is negatively regulated by fasting and food restriction, in all the models we studied: males, females and pregnant females. Next, we examined the effect of glucocorticoids and thyroid hormones on NPW mRNA expression in the stomach mucosa. Our data showed that NPW expression is decreased in this tissue after glucocorticoid treatment or hyperthyroidism. Conversely, hypothyroidism induces a marked increase in the expression of NPW in rat stomach. Overall, these data indicate that stomach NPW is regulated by nutritional and hormonal status.
Caminos
Jorge E
JE
Department of Physiology, School of Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain.
Bravo
Susana B
SB
García-Rendueles
María E R
ME
Ruth González
C
C
Garcés
Maria F
MF
Cepeda
Libia A
LA
Lage
Ricardo
R
Suárez
Miguel A
MA
López
Miguel
M
Diéguez
Carlos
C
D000818:Animals; D015153:Blotting, Western; D015536:Down-Regulation / Q000187:drug effects; D005215:Fasting / Q000502:physiology*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D005938:Glucocorticoids / Q000494:pharmacology*; D008297:Male; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D009752:Nutritional Status / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013963:Thyroid Hormones / Q000494:pharmacology*
D005938:Glucocorticoids; D009479:Neuropeptides; D012333:RNA, Messenger; D013963:Thyroid Hormones; C496874:neuropeptide W, rat