Halomonas kribbensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea.
A moderately halophilic, Gram-negative bacterium, designated strain BH843(T), was isolated from a solar saltern in Korea and subjected to a taxonomic analysis. Strain BH843(T) grew at salinities of 1-14 % (w/v) NaCl and at temperatures of 10-40 degrees C. The cells were motile cocci or short rods with single flagella and contained C(16 : 0), C(19 : 0) cyclo omega8c and C(17 : 0) cyclo as the major fatty acids. The G+C content of the genomic DNA was 66 mol% and the predominant ubiquinone was Q-9. Comparative 16S rRNA gene sequence analyses showed that strain BH843(T) formed a distinct phyletic line within the genus Halomonas, and the levels of 16S rRNA gene sequence similarity with respect to recognized Halomonas species were below 95.1 %. The levels of DNA-DNA relatedness between strain BH843(T) and the type strains of phylogenetically closely related Halomonas species were below 25 %. On the basis of phenotypic, chemotaxonomic and molecular data, strain BH843(T) represents a novel species within the genus Halomonas, for which the name Halomonas kribbensis is proposed. The type strain is BH843(T) (=KCTC 12584(T)=DSM 17892(T)).
Jeon
Che Ok
CO
Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
Lim
Jee-Min
JM
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Lee
Jung Ro
JR
Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
Lee
Gye Suk
GS
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Park
Dong-Jin
DJ
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Lee
Jae-Chan
JC
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Oh
Hyun-Woo
HW
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Kim
Chang-Jin
CJ
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D020632:Halomonas / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D007723:Korea; D008124:Locomotion / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D014451:Ubiquinone / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D012965:Sodium Chloride