title: Cytological diagnosis of basal cell carcinoma and actinic keratosis, using Papanicolaou and May-Grünwald-Giemsa stained cutaneous tissue smear.
abstract: OBJECTIVE
  Cytology may become the diagnostic method of choice with the advent of new non-invasive treatments for non-melanoma skin cancer, as the sampling technique for cytology entails little tissue disfiguration. The aim of this study was to compare and evaluate the diagnostic performance of scrape cytology using two different cytological staining techniques, and to evaluate additional touch imprint cytology, with that of histopathology of basal cell carcinoma (BCC) and actinic keratosis (AK).
  
  
  METHODS
  We investigated 50 BCC and 28 AK histologically verified lesions, from 41 and 25 patients, respectively. Two separate skin scrape samples and one touch imprint sample were taken from each lesion. The smears were stained with Papanicolaou (Pap) or May-Grünwald-Giemsa (MGG) stains. All cytological specimens were examined in random order by pathologists without knowledge of the histology. Cytodiagnostic results were compared with the histopathological report.
  
  
  RESULTS
  Scrape cytodiagnosis agreed with histopathology in 48 (Pap) and 47 (MGG) of the 50 BCC cases, and in 26 of 28 (Pap) and 21 of 26 (MGG) AK cases, yielding sensitivities of 96%, 94%, 93% and 81%, respectively. No significant difference in sensitivity between the two staining methods was found but a trend towards higher Pap sensitivity for AK was noted (P = 0.10). Touch imprint cytology confirmed histopathology in 38 of the 77 cases of BCC and AK.
  
  
  CONCLUSIONS
  Cytological diagnosis with either Pap or MGG stain for BCC and AK is reliable, and differentiates well between BCC and AK. Imprint cytology proved to be non-diagnostic in half of the examined cases.
authors.affiliation: Department of Dermatology, St. Olav's Hospital HF, Trondheim University Hospital, Trondheim, Norway. eidi.christensen@stolav.no
authors.forename: E
authors.initials: E
authors.lastname: Christensen
authors.forename: A
authors.initials: A
authors.lastname: Bofin
authors.forename: I
authors.initials: I
authors.lastname: Gudmundsdóttir
authors.forename: E
authors.initials: E
authors.lastname: Skogvoll
mesh_terms: D002280:Basal Cell Carcinoma / Q000175:diagnosis* / Q000473:pathology; D004396:Coloring Agents; D003581:Cytodiagnosis / Q000379:methods*; D004801:Eosine Yellowish-(YS) / Q000378:metabolism*; D005260:Female; D006801:Humans; D007642:Keratosis / Q000175:diagnosis* / Q000473:pathology; D008297:Male; D008751:Methylene Blue / Q000378:metabolism*; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000473:pathology; D013194:Staining and Labeling / Q000379:methods
chemical_list: D004396:Coloring Agents; C043260:May-Grunwald Giemsa; D008751:Methylene Blue; D004801:Eosine Yellowish-(YS)