title: High oxygen atmosphere improves human follicle development in organ cultures of ovarian cortical tissues in vitro.
abstract: BACKGROUND
  Obtaining mature human follicles from cultured ovarian tissue may be beneficial for clinical use for women who wish to preserve fertile competence. However, the methodology of culture such as culture condition and gas atmosphere has not been well established in humans. Therefore, we investigated the effect of oxygen concentration in organ culture in order to establish an ovarian tissue culture method.
  
  
  METHODS
  Ovarian tissue was obtained from 26-35-year-old women undergoing removal of a benign tumor (n = 12) or caesarean section (n = 16). The ovarian cortical tissues were cultured on a cell culture insert for 15 days under high (100%) and low (air, 20%) oxygen concentrations and then inspected for follicle development with light and electron microscopy. Estradiol and progesterone concentrations in the medium during culture were measured.
  
  
  RESULTS
  The ultrastructure and the function of hormone secretion in the cultured tissues were well preserved after organ culture. The follicles developing under high oxygen were larger and more matured than those developing under low oxygen (P < 0.05).
  
  
  CONCLUSIONS
  Human ovarian tissues can be cultured for 15 days under high oxygen concentration with the organ culture system used here. This technique could make it possible to utilize ovarian tissue for preservation of reproductive competence in cancer patients.
authors.affiliation: The Centre for Reproductive Medicine and Infertility, IVF Namba Clinic, 1-17-28, Minami-horie, Nishi-ku, Osaka 550-0015, Japan. york@ivfnamba.com
authors.forename: Y
authors.initials: Y
authors.lastname: Morimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Oku
authors.forename: M
authors.initials: M
authors.lastname: Sonoda
authors.forename: A
authors.initials: A
authors.lastname: Haruki
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: S
authors.initials: S
authors.lastname: Hashimoto
authors.forename: A
authors.initials: A
authors.lastname: Fukuda
mesh_terms: D000328:Adult; D003470:Culture Media / Q000494:pharmacology; D004967:Estrogens / Q000378:metabolism; D005260:Female; D006801:Humans; D008854:Microscopy, Electron; D009924:Organ Culture Techniques / Q000379:methods*; D006080:Ovarian Follicle / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D010100:Oxygen / Q000494:pharmacology*; D011374:Progesterone / Q000378:metabolism
chemical_list: D003470:Culture Media; D004967:Estrogens; D011374:Progesterone; D010100:Oxygen