Determination of alkali-labile phosphoprotein phosphorus from fish plasma using the Tb(3+)-tiron complex as a fluorescence probe.
A sensitive method based on the fluorescence quenching effect of the Tb(3+)-Tiron complex is proposed for the determination of alkali-labile phosphoprotein phosphorus (ALP) released from fish plasma. The detection limit was 5.4 ng/ml (S/N = 2), and the relative standard deviation of the quenching effect (6 replicates) was 4.6%. The results obtained by the proposed method were in good agreement with those obtained by the colorimetric assay. The advantages of the present method are its relatively simple detection procedure, the lack of toxic organic solvents, and high sensitivity.
Lv
Xue-Fei
XF
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. xuefeilv@163.com
Zhao
Yi-Bing
YB
Zhou
Qun-Fang
QF
Jiang
Gui-Bin
GB
Song
Mao-Yong
MY
D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt / Q000737:chemistry*; D000818:Animals; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D005399:Fishes / Q000097:blood*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008297:Male; D010750:Phosphoproteins / Q000097:blood*; D010758:Phosphorus / Q000097:blood*; D013725:Terbium / Q000737:chemistry*; D014819:Vitellogenins / Q000097:blood
D004967:Estrogens; D005456:Fluorescent Dyes; D010750:Phosphoproteins; D014819:Vitellogenins; D013725:Terbium; D010758:Phosphorus; D004958:Estradiol; D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt