title: Characterization of HIV-1 isolates from antiretroviral drug-naive children in southern India.
abstract: Access to antiretroviral therapy has expanded in many developing countries, including India. The standard first-line regimens consist of a combination of two nucleoside reverse transcriptase inhibitors and a nonnucleoside reverse transcriptase inhibitor, in a fixed drug combination. Data regarding resistance to these drugs are scarce, especially in children. We evaluated the pattern of polymorphism and potential drug resistance mutations (DRMs) in HIV-1 isolates from 48 children naive to antiretroviral therapy attending the outpatient clinics of the Tuberculosis Research Center in Chennai. The samples were subjected to genotyping of reverse transcriptase (RT) and protease genes. All the samples showed significant polymorphisms in both RT and protease genes, but none had major DRMs. The currently recommended generic first-line antiretroviral drug combination is an appropriate treatment strategy for HIV-1-infected children in India.
authors.affiliation: Division of HIV/AIDS, Tuberculosis Research Center, Chennai, India.
authors.forename: Lakshmi
authors.initials: L
authors.lastname: Soundararajan
authors.forename: Ramesh
authors.initials: R
authors.lastname: Karunaianandham
authors.forename: Valerie
authors.initials: V
authors.lastname: Jauvin
authors.forename: Marie Helene
authors.initials: MH
authors.lastname: Schrive
authors.forename: Ranjani
authors.initials: R
authors.lastname: Ramachandran
authors.forename: Paranji R
authors.initials: PR
authors.lastname: Narayanan
authors.forename: Herve J
authors.initials: HJ
authors.lastname: Fleury
authors.forename: Soumya
authors.initials: S
authors.lastname: Swaminathan
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D024882:Drug Resistance, Viral / Q000235:genetics*; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D016333:HIV Protease / Q000235:genetics; D054303:HIV Reverse Transcriptase / Q000235:genetics; D015497:HIV-1 / Q000145:classification* / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D007194:India / Q000453:epidemiology; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D019380:Anti-HIV Agents; D054303:HIV Reverse Transcriptase; D016333:HIV Protease