title: Borna disease virus RNA detected in Japanese macaques (Macaca fuscata).
abstract: We have examined the seroprevalence of BDV in wild Japanese macaques (Macaca fuscata) in the peninsula (Chiba prefecture), Japan. Serum samples from macaques were examined by the ELISA, Western blot and immunofluorescence assays to detect the presence of serum antibodies that react specifically to BDV antigens. Among 49 investigated individuals, 6 (12.2%) showed positive reaction to BDV antigens. RT-PCR studies detected BDV sequences in brain tissue of one case among four seropositive cases examined. Sequence analysis revealed a high degree of genetic conservation between BDV sequences derived from Japanese macaques and those documented for other animal species. Nevertheless, phylogenetic analysis revealed unique differences between macaque and other species derived BDV sequences.
authors.affiliation: School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. k-hagi@rakuno.ac.jp
authors.forename: Katsuro
authors.initials: K
authors.lastname: Hagiwara
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tsuge
authors.forename: Mitsuhiko
authors.initials: M
authors.lastname: Asakawa
authors.forename: Hajime
authors.initials: H
authors.lastname: Kabaya
authors.forename: Minoru
authors.initials: M
authors.lastname: Okamoto
authors.forename: Taku
authors.initials: T
authors.lastname: Miyasho
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Taniyama
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ishihara
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: de la Torre
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ikuta
mesh_terms: D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D001891:Borna disease virus / Q000235:genetics*; D016000:Cluster Analysis; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D007564:Japan; D008251:Macaca / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D016036:Seroepidemiologic Studies
chemical_list: D017931:DNA Primers; D012367:RNA, Viral