title: Influence of TGF-beta1 on the expression of BSP, DSP, TGF-beta1 receptor I and Smad proteins during reparative dentinogenesis.
abstract: Reparative dentin has a wide variety of manifestations ranging from a regular, tubular form to an irregular, atubular form. However, the characteristics of reparative dentin have not been clarified. This study hypothesized that the level of bone sialoprotein (BSP) expression will increase if the newly formed reparative dentin is bone-like but the dentin sialophosphoprotein (DSPP) level will decrease. In order to test this hypothesis, the expression of BSP and DSP was examined by immunohistochemistry and the expression of BSP was measured by in situ hybridization in an animal model. The pulps of 12 maxillary right first molars from twelve male rats were exposed and capped with MTA. In addition, in order to understand the role of transforming growth factor-beta 1 (TGF-beta1) during reparative dentinogenesis, the expression of BSP and DSPP mRNA was analyzed by RT-PCR in a human dental pulp cell culture, and the transforming growth factor-beta 1 receptors (TbetaRI) and Smad 2/3 were examined by immunofluorescence in an animal model. DSP was expressed in the normal odontoblasts and odontoblast-like cells of the reparative dentin. Interestingly, BSP was strongly expressed in the odontoblast-like cells of reparative dentin. The level of the TbetaRI and Smad 2/3 proteins was higher in the reparative dentin than in the normal dentin. TGF-beta1 up-regulated BSP in the human pulp cell cultures. This suggests that reparative dentin has both dentinogenic and osteogenic characteristics that are mediated by TGF-beta1.
authors.affiliation: Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea.
authors.forename: Yun-Chan
authors.initials: YC
authors.lastname: Hwang
authors.forename: In-Nam
authors.initials: IN
authors.lastname: Hwang
authors.forename: Won-Mann
authors.initials: WM
authors.lastname: Oh
authors.forename: Joo-Cheol
authors.initials: JC
authors.lastname: Park
authors.forename: Dong-Seol
authors.initials: DS
authors.lastname: Lee
authors.forename: Ho-Hyun
authors.initials: HH
authors.lastname: Son
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003782:Dental Pulp / Q000187:drug effects / Q000378:metabolism; D003804:Dentin / Q000033:anatomy & histology / Q000378:metabolism*; D003810:Dentinogenesis / Q000235:genetics; D016326:Extracellular Matrix Proteins; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D058574:Integrin-Binding Sialoprotein; D008297:Male; D010750:Phosphoproteins; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism; D012795:Sialoglycoproteins / Q000235:genetics / Q000378:metabolism*; D051899:Smad2 Protein / Q000378:metabolism; D051900:Smad3 Protein / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology / Q000502:physiology*; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D058574:Integrin-Binding Sialoprotein; D010750:Phosphoproteins; D011498:Protein Precursors; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta; D012795:Sialoglycoproteins; D051899:Smad2 Protein; D051900:Smad3 Protein; D053773:Transforming Growth Factor beta1; D000099311:Dentin Sialophosphoprotein; C546871:IBSP protein, human; C000625843:Tgfbr1 protein, rat