Preparation of tiny biodegradable capsules using electrocapillary emulsification.
Conditions for preparing tiny biodegradable capsules were examined using electrocapillary emulsification that allows one to prepare monodisperse emulsions with ease by applying a DC potential between oil and water phases without mechanical agitation. The results obtained showed that a 1 : 4 mixture of polysorbate 80 (TO-10), a hydrophilic non-ionic surfactant, and sorbitan monooleate (SO-10), an oleophilic surfactant, is appropriate as the surfactant to be added to oil phase, cyclohexane is acceptable as the oil phase and 1000 V is optimum as the DC potential to be applied. The capsules prepared had sizes ranging from 100-300 nm and a surface roughness of approximately 10 nm and degraded in model intestinal juice more easily and rapidly than in model gastric juice. In addition, the capsules containing lactoferrin, an anti-carcinogenic protein, were found to keep 12.5% of the protein used in encapsulation without losing its activity.
Abe
Masahiko
M
Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba, Japan. abemasa@rs.noda.tus.ac.jp
Nakayama
Ai
A
Kondo
Tamotsu
T
Morishita
Hiroshi
H
Tsuchiya
Koji
K
Utsumi
Shigenori
S
Ohkubo
Takahiro
T
Sakai
Hideki
H
D003510:Cyclohexanes / Q000737:chemistry*; D004563:Electrochemistry; D004655:Emulsions / Q000737:chemistry; D005750:Gastric Juice / Q000737:chemistry; D006601:Hexoses / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011136:Polysorbates / Q000737:chemistry*
D003510:Cyclohexanes; D004655:Emulsions; D006601:Hexoses; D053769:Nanocapsules; D011136:Polysorbates; C018665:sorbitan monooleate; C506365:Cyclohexane