The neuroprotective potential of phase II enzyme inducer on motor neuron survival in traumatic spinal cord injury in vitro.
(1) Phase II enzyme inducer is a kind of compound which can promote the expression of antioxidative enzymes through nuclear factor erythroid 2-related factor 2 (Nrf2) activation. Recently, it has been reported that these compounds show neuroprotective effect via combating oxidative stress. The purpose of this study is to determine whether phase II enzyme inducers have neuroprotective effects on traumatic spinal cord injury. (2) An organotypic spinal cord culture system was used, Phase II enzyme inducers were added to culture medium for 1 week, motor neurons were counted by SMI-32 staining, glutamate, Nrf2, and Heme oxygenase-1(HO-1) mRNA were tested. (3) This study showed motor neuron loss within 1 week in culture. After 1 week in culture, the system was stable. Moreover, Glutamate was increased when in culture 48 h and decreased after 1 week in culture. There was no significant change between 1 and 4 weeks in culture. Necrotic motor neuron and damaged mitochondrial were observed in culture 48 h. Furthermore, phase II enzyme inducers: tert-butyhydroquinone (t-BHQ), 3H-1,2-dithiole-3-thione (D3T), and 5,6-dihydrocyclopenta-1,2-dithiole-3-thione (CPDT) were shown to promote motor neuron survival after dissection, it was due to increasing Nrf2 and HO-1 mRNA expression and protecting mitochondrial not due to decreasing glutamate level. (4) The loss of motor neuron due to dissection can mimic severe traumatic spinal cord injury. These results demonstrate that glutamate excitotoxicity and the damage of mitochondrial is possibly involve in motor neuron death after traumatic spinal cord injury and phase II enzyme inducers show neuroprotective potential on motor neuron survival in traumatic spinal cord injury in vitro.
Liu
Xiao-Yun
XY
Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, P.R. China.
Li
Chun-Yan
CY
Bu
Hui
H
Li
Zhe
Z
Li
Bin
B
Sun
Meng-Meng
MM
Guo
Yan-Su
YS
Zhang
Li
L
Ren
Wen-Bo
WB
Fan
Zhi-Liang
ZL
Wu
Dong-Xia
DX
Wu
Shu-Yu
SY
D000818:Animals; D000831:Animals, Newborn; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D004790:Enzyme Induction / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018698:Glutamic Acid / Q000378:metabolism; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics; D006873:Hydroquinones / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009046:Motor Neurons / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D051267:NF-E2-Related Factor 2 / Q000235:genetics; D009410:Nerve Degeneration / Q000188:drug therapy* / Q000201:enzymology / Q000517:prevention & control; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009924:Organ Culture Techniques; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000187:drug effects* / Q000201:enzymology / Q000503:physiopathology; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000201:enzymology / Q000503:physiopathology; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013871:Thiones / Q000494:pharmacology
C522589:5,6-dihydrocyclopenta-1,2-dithiole-3-thione; D004791:Enzyme Inhibitors; D006873:Hydroquinones; D051267:NF-E2-Related Factor 2; D018696:Neuroprotective Agents; C495637:Nfe2l2 protein, rat; D012333:RNA, Messenger; D013438:Sulfhydryl Compounds; D013871:Thiones; D018698:Glutamic Acid; C018855:2-tert-butylhydroquinone; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat