title: Comparative in vitro study of the proliferation and growth of ovine osteoblast-like cells on various alloplastic biomaterials manufactured for augmentation and reconstruction of tissue or bone defects.
abstract: In this in vitro study ovine osteoblast-like cells were cultured on seven different alloplastic biomaterials used for augmentation and for reconstruction of bone defects in dental and craniomaxillofacial surgery. The aim of this study was to examine the growth behaviour (viability, cell density and morphology) of ovine osteoblast-like cells on the investigated biomaterials to get knowledge which biomaterial is qualified to act as a cell carrier system in further in vivo experiments. The biomaterials were either synthetically manufactured or of natural origin. As synthetically manufactured biomaterials Ethisorb, MakroSorb, PalacosR, and PDS film were used. As biomaterials of natural origin BeriplastP, Bio-Oss and Titanmesh were investigated. The cell proliferation and cell colonization were analyzed by a proliferation assay and scanning electron microscopy. Osteoblast-like cells proliferated and attached on all biomaterials, except on Beriplast. On Ethisorb the highest cell proliferation rate was measured followed by PalacosR. Both biomaterials offer suitable growth and proliferation conditions for ovine osteoblast-like cells. The proliferation rates of Bio-Oss, MakroSorb, PDS-film and Titanmesh were low and SEM examinations of these materials showed less spread osteoblast-like cells. The results showed that ovine osteoblast-like cells appear to be sensitive to substrate composition and topography. This in vitro study provides the basis for further in vivo studies using the sheep model to examine the biocompatibility and the long-term interaction between the test material and tissue (bone regeneration).
authors.affiliation: Klinik für Mund-, Kiefer- und Gesichtschirurgie, Universitätsklinikum Freiburg, Hugstetterstrasse 55, Freiburg 79106, Germany.
authors.forename: Sandra C
authors.initials: SC
authors.lastname: Schmitt
authors.forename: Margit
authors.initials: M
authors.lastname: Wiedmann-Al-Ahmad
authors.forename: Jens
authors.initials: J
authors.lastname: Kuschnierz
authors.forename: Ali
authors.initials: A
authors.lastname: Al-Ahmad
authors.forename: Ute
authors.initials: U
authors.lastname: Huebner
authors.forename: Rainer
authors.initials: R
authors.lastname: Schmelzeisen
authors.forename: Ralf
authors.initials: R
authors.lastname: Gutwald
mesh_terms: D000497:Alloys / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001847:Bone Diseases / Q000628:therapy; D001861:Bone Regeneration / Q000187:drug effects*; D016723:Bone Remodeling / Q000187:drug effects; D049108:Cell Enlargement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D015675:Osteocalcin / Q000378:metabolism; D010969:Plastics / Q000737:chemistry / Q000494:pharmacology*; D012756:Sheep; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D010969:Plastics; D015675:Osteocalcin