title: Catechin degradation with concurrent formation of homo- and heterocatechin dimers during in vitro digestion.
abstract: Catechins were subjected to in vitro gastric and small intestinal digestion. EGCG, EGC, and ECG were significantly degraded at all concentrations tested, with losses of 71-91, 72-100, and 60-61%, respectively. EC and C were comparatively stable, with losses of 8-11 and 7-8%, respectively. HLPC-ESI-MS/MS indicated that EGCG degradation under simulated digestion resulted in production of theasinensins (THSNs) A and D (m/z 913) and P-2 (m/z 883), its autoxidation homodimers. EGC dimerization produced the homodimers THSN C and E (m/z 609) and homodimers analogous to P-2 (m/z 579). ECG homodimers were not observed. EGCG and EGC formed heterodimers analogous to the THSNs (m/z 761) and P-2 (m/z 731). EGCG and ECG formed homodimers analogous to the THSNs (m/z 897). This study provides an expanded profile of catechin dimers of digestive origin that may potentially form following consumption of catechins. These data provide a logical basis for initial screening to detect catechin digestive products in vivo.
authors.affiliation: Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, Indiana 47907, USA
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Neilson
authors.forename: Amber S
authors.initials: AS
authors.lastname: Hopf
authors.forename: Bruce R
authors.initials: BR
authors.lastname: Cooper
authors.forename: Michael A
authors.initials: MA
authors.lastname: Pereira
authors.forename: Joshua A
authors.initials: JA
authors.lastname: Bomser
authors.forename: Mario G
authors.initials: MG
authors.lastname: Ferruzzi
mesh_terms: D002392:Catechin / Q000031:analogs & derivatives / Q000378:metabolism*; D004063:Digestion; D019281:Dimerization; D066298:In Vitro Techniques; D008049:Lipase / Q000378:metabolism; D010084:Oxidation-Reduction; D010194:Pancreatin / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism
chemical_list: D010194:Pancreatin; D002392:Catechin; C062669:epicatechin gallate; C045651:epigallocatechin gallate; D008049:Lipase; D010434:Pepsin A; C057580:gallocatechol