Gene expression profile of macrophage-like U937 cells in response to polyethylene particles: a novel cell-particle culture system.
We investigated the gene expression profiles of U937 cells after contact with polyethylene particles. U937 cells were differentiated with phorbol 12-myristate 13-acetate, and cocultured with either retrieved polyethylene particles or commercially produced polyethylene particles (Ceridust 3615, Clariant Japan, Tokyo, Japan). To achieve consistent contact with the polyethylene particles, we used a rotating device. Phagocytosis of the polyethylene particles or retrieved polyethylene particles by differentiated U937 cells stimulated the release of cytokines including interleukin 1beta, interleukin 6, interleukin 8, and vascular endothelial growth factor. Microarray analysis revealed that the expression of IL8, CCL4, CXCR4, and some other genes was up-regulated after contact with retrieved polyethylene particles. This study first reports the gene expression profiles of U937 cells after contact with polyethylene particles. We believe that this experimental model is applicable to all other particulate materials.
Matsusaki
Takashi
T
Department of Orthopaedic Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Kawanabe
Keiichi
K
Ise
Kentaro
K
Nakayama
Tomitaka
T
Toguchida
Junya
J
Nakamura
Takashi
T
D054407:Chemokine CCL4 / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D008863:Microspheres; D020411:Oligonucleotide Array Sequence Analysis; D010587:Phagocytosis / Q000502:physiology; D020959:Polyethylene; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism; D020298:U937 Cells; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
D054407:Chemokine CCL4; D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D012333:RNA, Messenger; D019718:Receptors, CXCR4; D042461:Vascular Endothelial Growth Factor A; D020959:Polyethylene