title: Genomic expression patterns in cell separation mutants of Schizosaccharomyces pombe defective in the genes sep10 ( + ) and sep15 ( + ) coding for the Mediator subunits Med31 and Med8.
abstract: Cell division is controlled by a complex network involving regulated transcription of genes and postranslational modification of proteins. The aim of this study is to demonstrate that the Mediator complex, a general regulator of transcription, is involved in the regulation of the second phase (cell separation) of cell division of the fission yeast Schizosaccharomyces pombe. In previous studies we have found that the fission yeast cell separation genes sep10 ( + ) and sep15 ( + ) code for proteins (Med31 and Med8) associated with the Mediator complex. Here, we show by genome-wide gene expression profiling of mutants defective in these genes that both Med8 and Med31 control large, partially overlapping sets of genes scattered over the entire genome and involved in diverse biological functions. Six cell separation genes controlled by the transcription factors Sep1 and Ace2 are among the target genes. Since neither sep1 ( + ) nor ace2 ( + ) is affected in the mutant cells, we propose that the Med8 and Med31 proteins act as coactivators of the Sep1-Ace2-dependent cell separation genes. The results also indicate that the subunits of Mediator may contribute to the coordination of cellular processes by fine-tuning of the expression of larger sets of genes.
authors.affiliation: Department of Genetics and Applied Microbiology, University of Debrecen, Debrecen, Hungary.
authors.forename: Ida
authors.initials: I
authors.lastname: Miklos
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Szilagyi
authors.forename: Stephen
authors.initials: S
authors.lastname: Watt
authors.forename: Erika
authors.initials: E
authors.lastname: Zilahi
authors.forename: Gyula
authors.initials: G
authors.lastname: Batta
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Antunovics
authors.forename: Klara
authors.initials: K
authors.lastname: Enczi
authors.forename: Jürg
authors.initials: J
authors.lastname: Bähler
authors.forename: Matthias
authors.initials: M
authors.lastname: Sipiczki
mesh_terms: D001483:Base Sequence; D002455:Cell Division / Q000235:genetics*; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000235:genetics; D003599:Cytoskeleton / Q000235:genetics / Q000648:ultrastructure; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D020869:Gene Expression Profiling; D005800:Genes, Fungal; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D021122:Protein Subunits; D012326:RNA Splicing / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D021122:Protein Subunits; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors