title: Cellular dynamics in the draining lymph nodes during sensitization and elicitation phases of contact hypersensitivity.
abstract: BACKGROUND
  The different role of various immunological effector cells in contact hypersensitivity (CHS) is receiving increased attention. During the past decade, the involvement of different cell types in CHS has been investigated by the use of antibody-induced depletion of specific subtypes of immunological cells and by studying knockout mice lacking one or more of these immunological cell populations.
  
  
  OBJECTIVE
  To develop a method for studying the collective cellular dynamics of immune cells in the draining lymph nodes during CHS in intact animals.
  
  
  METHODS
  Mice were sensitized and/or challenged with 2,4-dinitrofluorobenzene or oxazolone. Using multi-parameter flow cytometry we determined the proliferation, activation state, and absolute number of helper T cells, cytotoxic T cells, B cells, and natural killer cells in the draining lymph nodes.
  
  
  RESULTS
  The presented method can be applied to evaluate the effect of different contact allergens on various cell populations of the immune system.
  
  
  CONCLUSIONS
  Our study support recent findings that several cell types seem to be involved in CHS.
authors.affiliation: Section of Experimental Immunology, Department of International Health, Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, DK-2200 Copenhagen, Denmark.
authors.forename: Jeppe Madura
authors.initials: JM
authors.lastname: Larsen
authors.forename: Carsten
authors.initials: C
authors.lastname: Geisler
authors.forename: Martin Weiss
authors.initials: MW
authors.lastname: Nielsen
authors.forename: Lasse
authors.initials: L
authors.lastname: Boding
authors.forename: Marina
authors.initials: M
authors.lastname: Von Essen
authors.forename: Ann Kathrine
authors.initials: AK
authors.lastname: Hansen
authors.forename: Lone
authors.initials: L
authors.lastname: Skov
authors.forename: Charlotte Menné
authors.initials: CM
authors.lastname: Bonefeld
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology; D017449:Dermatitis, Allergic Contact / Q000276:immunology*; D004139:Dinitrofluorobenzene / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology / Q000502:physiology; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D010081:Oxazolone / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000502:physiology
chemical_list: D000485:Allergens; D000906:Antibodies; D010081:Oxazolone; D004139:Dinitrofluorobenzene