Monitoring the interactions of tocopherol homologues with reversed-phase stationary HPLC phases by 1H suspended-state saturation transfer difference high-resolution/magic angle spinning NMR spectroscopy.
The separation process in reversed-phase high-performance liquid chromatography employing C18 phases is mainly due to hydrophobic interactions. The separation of tocopherol isomers, exhibited by the C30 phases, however, is additionally driven by shape selectivity. This phenomenon is investigated by suspended-state nuclear magnetic resonance spectroscopy using the saturation transfer difference technique, which was originally introduced to study protein-ligand interactions. The interaction strength between beta-/gamma-tocopherol and three different stationary phases was estimated qualitatively. The nuclear magnetic resonance data are compared to chromatographic data, and a similar mode of interaction between the analytes and the stationary phases is elucidated.
Schauff
Siri
S
Institut für Organische Chemie, University of Tübingen, Auf der Morgenstelle 18, 72076 Tübingen, Germany.
Friebolin
Volker
V
Grynbaum
Marc David
MD
Meyer
Christoph
C
Albert
Klaus
K
D000180:Acrylic Resins / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure; D010316:Particle Size; D011095:Polyethylenes / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D024505:Tocopherols / Q000032:analysis*
D000180:Acrylic Resins; D011095:Polyethylenes; C083316:(polyethylene-acrylic acid) copolymer; D024505:Tocopherols