Isolation of genes expressed during the developmental stages of the oyster mushroom, Pleurotus ostreatus, using expressed sequence tags.
In order to isolate and identify the developmentally regulated genes during fruiting body development, cDNA libraries were constructed from eight developmental stages of the Oyster mushroom, Pleurotus ostreatus. From these libraries, 11 761 expressed sequence tags (PoESTs) were generated. Of these, 4060 different genes (PoUnigenes) were identified, representing 34.5% of the entire genome. Redundancy analysis of ESTs during the developmental stages identified eight, 13 and two genes that were specifically expressed in mycelia, fruiting body and basidiospore, respectively. RT-PCR was used to confirm the specific expression of nine genes which showed specific redundancy in fruiting body stages, four genes of which were expressed specifically in fruiting body stages as expected in redundancy analysis, and other genes were expressed abundantly in fruiting body stages. The EST database of P. ostreatus generated during this study provides a genetic and biochemical basis for future studies of the developmental stages of basidiomycetes.
Joh
Joong-Ho
JH
Department of Applied Biochemistry, College of Natural Science, Konkuk University, Chung-Ju, Korea.
Lee
Seung-Ho
SH
Lee
Jin-sung
JS
Kim
Ki-Hwan
KH
Jeong
Sung-Ja
SJ
Youn
Woong-Han
WH
Kim
Nam-Kuk
NK
Son
Eun-Suk
ES
Cho
Yong-Sup
YS
Yoo
Young-Bok
YB
Lee
Chang-Soo
CS
Kim
Beom-Gi
BG
D004271:DNA, Fungal / Q000235:genetics; D020224:Expressed Sequence Tags; D048690:Fruiting Bodies, Fungal / Q000235:genetics / Q000254:growth & development; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D005800:Genes, Fungal; D025282:Mycelium / Q000235:genetics / Q000254:growth & development; D020076:Pleurotus / Q000235:genetics* / Q000254:growth & development*; D012331:RNA, Fungal / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development
D004271:DNA, Fungal; D012331:RNA, Fungal; D012333:RNA, Messenger