title: Effect of oxidative stress and involvement of poly(ADP-ribose) polymerase (PARP) in Dictyostelium discoideum development.
abstract: Dictyostelium discoideum, a unicellular eukaryote, exhibits multicellularity upon nutrient starvation and is a good model system for developmental studies, and for the study of various signal transduction pathways. Reactive oxygen species at low doses act as signaling molecules; however, at high doses they are known to cause DNA damage that results in the activation of poly(ADP-ribose) polymerase (PARP). We have earlier reported the high resistance of the unicellular stage of D. discoideum to oxidative stress, and we now show the response of this organism to oxidative stress and the role of PARP during development. We used hydroxylamine (HA) to induce in situ generation of H(2)O(2) and monitored the effect of benzamide, a PARP inhibitor, on oxidative stress-induced changes in D. discoideum development. Interestingly, oxidative stress resulted in PARP activation within 5 min that was inhibited by benzamide. Oxidative stress-induced delay in developmental pattern was also partially restored by benzamide. We studied the long-term effects of PARP inhibition under oxidative stress, and our results demonstrated that spores formed under HA stress exhibited significant delay in germination in comparison to benzamide-pretreated HA-stressed cells. However, second-generation cells showed normal development, signifying that PARP inhibition has no deleterious effect on D. discoideum development under oxidative stress.
authors.affiliation: Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, India.
authors.forename: Jyotika
authors.initials: J
authors.lastname: Rajawat
authors.forename: Iqbal
authors.initials: I
authors.lastname: Vohra
authors.forename: Hina A
authors.initials: HA
authors.lastname: Mir
authors.forename: Dhaval
authors.initials: D
authors.lastname: Gohel
authors.forename: Rasheedunnisa
authors.initials: R
authors.lastname: Begum
mesh_terms: D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D016923:Cell Death; D004023:Dictyostelium / Q000201:enzymology* / Q000254:growth & development*; D004789:Enzyme Activation; D018384:Oxidative Stress; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D015800:Protozoan Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D033761:Spores, Protozoan / Q000254:growth & development
chemical_list: D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D015800:Protozoan Proteins; C037689:benzamide; D011065:Poly(ADP-ribose) Polymerases