Transcription alters chromosomal locations of cohesin in Saccharomyces cerevisiae.
In eukaryotic cells, cohesion between sister chromatids allows chromosomes to biorient on the metaphase plate and holds them together until they separate into daughter cells during mitosis. Cohesion is mediated by the cohesin protein complex. Although the association of this complex with particular regions of the genome is highly reproducible, it is unclear what distinguishes a chromosomal region for cohesin association. Since one of the primary locations of cohesin is intergenic regions between converging transcription units, we explored the relationship between transcription and cohesin localization. Chromatin immunoprecipitation followed by hybridization to a microarray (ChIP chip) indicated that transcript elongation into cohesin association sites results in the local disassociation of cohesin. Once transcription is halted, cohesin can reassociate with its original sites, independent of DNA replication and the cohesin loading factor Scc2, although cohesin association with chromosomes in G2/M is not functional for cohesion. A computer program was developed to systematically identify differences between two ChIP chip data sets. Our results are consistent with a model for cohesin association in which (i) a portion of cohesin can be dynamically loaded and unloaded to accommodate transcription and (ii) the cohesin complex has preferences for features of chromatin that are a reflection of the local transcriptional status. Taken together, our results suggest that cohesion may be degraded by transcription.
Bausch
Christoph
C
Stowers Institute for Medical Research, 1000 E. 50th St., Kansas City, MO 64110, USA.
Noone
Seth
S
Henry
Jill M
JM
Gaudenz
Karin
K
Sanderson
Brian
B
Seidel
Chris
C
Gerton
Jennifer L
JL
D000596:Amino Acids / Q000172:deficiency; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D015825:Chromosomes, Fungal / Q000378:metabolism*; D016195:G2 Phase / Q000187:drug effects; D005690:Galactose / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D000097722:Cohesins
D000596:Amino Acids; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D005690:Galactose; D009004:Monosaccharide Transport Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; D000097722:Cohesins; C106901:GAL2 protein, S cerevisiae; C490529:MCD1 protein, S cerevisiae; C410592:SCC2 protein, S cerevisiae