Assessment of prion inactivation by combined use of Bacillus-derived protease and SDS.
Prions, infectious agents causing transmissible spongiform encephalopathy, retain infectivity even after undergoing routine sterilization processes. We found that MSK103 protease, identified in our previous study, effectively reduces infectivity and the level of misfolded isoform of the prion protein in scrapie-infected brain homogenates in the presence of SDS. The treatment therefore can be applied to the decontamination of thermolabile instruments.
Yoshioka
Miyako
M
Prion Disease Research Center, National Institute of Animal Health, Japan.
Murayama
Yuichi
Y
Miwa
Takehiro
T
Miura
Katsuhiro
K
Takata
Masuhiro
M
Yokoyama
Takashi
T
Nishizawa
Koji
K
Mohri
Shirou
S
D000818:Animals; D001407:Bacillus / Q000201:enzymology*; D004357:Drug Synergism; D006863:Hydrogen-Ion Concentration; D007276:Injections, Intraventricular; D051379:Mice; D008822:Mice, Transgenic; D010447:Peptide Hydrolases / Q000302:isolation & purification / Q000494:pharmacology*; D018620:PrPSc Proteins / Q000008:administration & dosage / Q000378:metabolism*; D011489:Protein Denaturation; D020033:Protein Isoforms; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D013242:Sterilization / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D018620:PrPSc Proteins; D020033:Protein Isoforms; D012967:Sodium Dodecyl Sulfate; D010447:Peptide Hydrolases