Unique features and distribution of the chicken CD83+ cell.
The central importance of dendritic cells (DC) in both innate and acquired immunity is well recognized in the mammalian immune system. By contrast DC have yet to be characterized in avian species despite the fact that avian species such as the chicken have a well-developed immune system. CD83 has proven to be an excellent marker for DC in human and murine immune systems. In this study we identify chicken CD83 (chCD83) as the avian equivalent of the human and murine DC marker CD83. We demonstrate for the first time that unlike human and murine CD83, chCD83 is uniquely expressed in the B cell areas of secondary lymphoid organs and in organs with no human or murine equivalent such as the bursa and Harderian gland. Furthermore through multicolor immunofluorescence, we identify chCD83(+) populations that have unique attributes akin to both DC and follicular DC. These attributes include colocalization with B cell microenvironments, MHC class II expression, dendritic morphology, and distribution throughout peripheral and lymphoid tissues.
Hansell
Chris
C
Department of Veterinary Medicine, Centre for Veterinary Science, University of Cambridge, Cambridge, United Kingdom. ch120j@clinmed.gla.ac.uk
Zhu
Xing Wu
XW
Brooks
Heather
H
Sheppard
Mark
M
Withanage
Sam
S
Maskell
Duncan
D
McConnell
Ian
I
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D015415:Biomarkers / Q000737:chemistry / Q000378:metabolism; D002060:Bursa of Fabricius / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002645:Chickens; D003713:Dendritic Cells / Q000166:cytology; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000276:immunology; D012486:Salmonella typhimurium / Q000235:genetics; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D000097802:CD83 Antigen
D015703:Antigens, CD; D015415:Biomarkers; D000949:Histocompatibility Antigens Class II; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen