title: [Subtype and sequence analysis of gag and env genes among HIV-1 strains circulating in Beijing residents during 2006].
abstract: OBJECTIVE
  To investigate the subtype distribution and the prevalence of sequence characteristics of HIV-1 strains in Beijing residents during 2006 and to analyze the relationship between distribution of HIV-1 subtypes and transmission routines.
  
  
  METHODS
  Blood samples from 32 new confirmed HIV-1 infected individuals from Beijing residents in 2006 and separated plasma specimens were collected. RNAs were extracted and the gag and env gene were amplified by RT-PCR and nest-PCR. PCR products were sequenced directly and phylogenetic analyses of gag and env gene were performed using the MEGA2 software.
  
  
  RESULTS
  Among 32 HIV-1 plasma samples, 22 gag and 4 env gene fragments were amplified and analyzed. Five HIV-1 subtypes or circulating recombinant forms(CRFs) of HIV-1 including Thai B (2 strains), B (9 strains), C (2 strains), CRF07_BC (5 strains), CRF01 AE (4 strains) were identified being circulated in Beijing. The gene divergences of gag gene inside the subtypes were 6.6%, 4.3%, 6.8%, 4.9% and 3.0% in subtype B, Thai B, C, CRF01_AE and CRF07_BC respectively. Subtypes B were predominant in Beijing, accounted for 40.9% among 22 samples.
  
  
  CONCLUSIONS
  Five HIV-1 subtypes were identified in Beijing and the surveillance of HIV-1 gene variation should be paid more attention to.
authors.affiliation: Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Jing-rong
authors.initials: JR
authors.lastname: Ye
authors.forename: Hui
authors.initials: H
authors.lastname: Xing
authors.forename: Hai-lin
authors.initials: HL
authors.lastname: Liu
authors.forename: Fa-xin
authors.initials: FX
authors.lastname: Hei
authors.forename: Yue-juan
authors.initials: YJ
authors.lastname: Zhao
authors.forename: Sheng-ya
authors.initials: SY
authors.lastname: Liu
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Sun
authors.forename: Qi-yun
authors.initials: QY
authors.lastname: Zhang
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Lu
authors.forename: Xiong
authors.initials: X
authors.lastname: He
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Shao
mesh_terms: D002681:China; D015497:HIV-1 / Q000145:classification* / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics*; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
chemical_list: D054299:env Gene Products, Human Immunodeficiency Virus; D054301:gag Gene Products, Human Immunodeficiency Virus