title: Simultaneous chiral resolution of monosaccharides as 8-aminonaphthalene-1,3,6-trisulfonate derivatives by ligand-exchange CE using borate as a central ion of the chiral selector.
abstract: Six reducing monosaccharides (mannose, galactose, fucose, glucose, xylose, and arabinose) were derivatized with 8-aminonaphthalene-1,3,6-trisulfonate (ANTS). Based on the chiral ligand-exchange principle using borate as a central ion of the chiral selector and (S)-3-amino-1,2-propanediol (SAP) as a chiral selector ligand, all of the six ANTS-monosaccharides were simultaneously enantioseparated using absorbance at 245 nm for detection. The optimum conditions for both high resolution and moderately short migration time consisted of 200 mM SAP-200 mM borate buffer (pH 9.2) containing 10% ACN as a BGE at 30 degrees C with an applied voltage of +30 kV. It was revealed that the proposed chiral ligand-exchange CE using the SAP-borate system was applicable to enantioseparation of not only diols but also polyols.
authors.affiliation: Toyama Institute of Health, Imizu, Toyama, Japan. shuji.kodama@pref.toyama.lg.jp
authors.forename: Shuji
authors.initials: S
authors.lastname: Kodama
authors.forename: Sen-ichi
authors.initials: S
authors.lastname: Aizawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Taga
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Yamashita
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kemmei
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Hayakawa
mesh_terms: D001881:Borates / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D015394:Molecular Structure; D009005:Monosaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D009281:Naphthalenes / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism
chemical_list: D001881:Borates; D008024:Ligands; D009005:Monosaccharides; D009281:Naphthalenes; C046465:8-amino-1,3,6-naphthalenetrisulfonic acid