Comparison of blood feeding response and infection of Aedes aegypti to Wuchereria bancrofti using animal membranes and direct host contact.
Comparison of an artificial, whole-blood membrane feeding procedure was performed by feeding Aedes aegypti (Liverpool strain) on the blood of patients infected with Wuchereria bancrofti microfilariae with the use of 3 types of membranes produced from chicken and mouse skin and swine intestine. Direct feeding of Ae. aegypti on the skin of infected human patients served as control. For all 3 types of membranes, mosquito survival, infection, and number of infective-stage larvae per mosquito did not differ significantly from the control. However, the blood feeding response between swine intestine layer (32%) compared to chicken skin (75.3%), mouse skin (70%), and direct feeding (84%) differed significantly. The response in direct feeding method was significantly higher than those in all membranes tested (F = 18.89; df = 3; P < 0.05) Chicken skin preparation was shown to be the preferred membrane for blood feeding Ae. aegypti and experimental infection with W. bancrofti.
Pothikasikorn
Jinrapa
J
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400 Thailand.
Bangs
Michael J
MJ
Chareonviriyaphap
Theeraphap
T
Roongruangchai
Kosol
K
Roongruangchai
Jantima
J
D000330:Aedes / Q000502:physiology*; D000818:Animals; D001769:Blood / Q000469:parasitology*; D002645:Chickens; D005247:Feeding Behavior / Q000502:physiology; D005368:Filariasis / Q000635:transmission; D006801:Humans; D007299:Insect Bites and Stings; D007422:Intestines; D051379:Mice; D012867:Skin; D013552:Swine; D014958:Wuchereria bancrofti