[Theranostic application of telomerase-specific oncolytic adenovirus for human cancer].
Replication-selective tumor-specific viruses present a novel approach for treatment of neoplastic disease. Telomerase activation is considered to be a critical step in carcinogenesis and its activity correlates closely with human telomerase reverse transcriptase (hTERT) expression. We constructed an attenuated adenovirus 5 vector (Telomelysin, OBP-301), in which the hTERT promoter element drives expression of E1 genes. We further modified the E3 region of OBP-301 to contain green fluorescent protein (GFP) gene for monitoring viral replication (TelomeScan, OBP-401). When TelomeScan was intratumorally injected into human tumors orthotopically implanted into the rectum in mice, para-aortic lymph node metastasis could be visualized at laparotomy under a three-chip color cooled charged-coupled device camera. This article reviews recent highlights in this rapidly evolving field: cancer therapeutic and cancer diagnostic approaches using the telomerase-specific oncolytic adenoviruses.
Fujiwara
Toshiyoshi
T
Center for Gene and Cell Therapy, Okayama University Hospital.
Tanaka
Noriaki
N
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000175:diagnosis; D050130:Oncolytic Virotherapy / Q000379:methods*; D050504:Oncolytic Viruses / Q000235:genetics*; D010446:Peptide Fragments / Q000032:analysis; D019098:Telomerase / Q000032:analysis* / Q000235:genetics*
D010446:Peptide Fragments; D049452:Green Fluorescent Proteins; C437311:telomerase reverse transcriptase (540-548); D019098:Telomerase