title: Leucine-rich repeat C4 protein is involved in nervous tissue development and neurite outgrowth, and induction of glioma cell differentiation.
abstract: LRRC4, leucine-rich repeat C4 protein, has been identified in human (GenBank accession No. AF196976), mouse (GenBank accession No. DQ177325), rat (GenBank accession No. DQ119102) and bovine (GenBank accession No. DQ164537) with identical domains. In terms of their similarity, the genes encoding LRRC4 in these four mammalian species are orthogs and therefore correspond to the same gene entity. Based on previous research, and using in situ hybridization, we found that LRRC4 had the strongest expression in hippocampal CA1 and CA2, the granule cells of the dentate gyrus region, the mediodoral thalamic nucleus, and cerebella Purkinje cell layers. Using a P19 cell model, we also found that LRRC4 participates in the differentiation of neuron and glia cells. In addition, extracellular proteins containing both an LRR cassette and immunoglobulin domains have been shown to participate in axon guidance. Our data from neurite outgrowth assays indicated that LRRC4 promoted neurite extension of hippocampal neurons, and induced differentiation of glioblastoma U251 cells into astrocyte-like cells, confirmed by morphology observation and glial fibrillary acidic protein expression.
authors.affiliation: Cancer Research Institute, Central South University, Changsha 410078, China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Wu
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Chen
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.forename: Jianhong
authors.initials: J
authors.lastname: Lu
authors.forename: Shourong
authors.initials: S
authors.lastname: Shen
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000166:cytology; D002417:Cattle; D002454:Cell Differentiation / Q000502:physiology*; D045744:Cell Line, Tumor; D005910:Glioma / Q000737:chemistry* / Q000473:pathology; D006624:Hippocampus / Q000737:chemistry / Q000166:cytology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009417:Nerve Tissue / Q000737:chemistry* / Q000166:cytology / Q000196:embryology*; D009419:Nerve Tissue Proteins / Q000502:physiology*; D016501:Neurites / Q000737:chemistry / Q000378:metabolism*; D051381:Rats
chemical_list: C503332:LRRC4 protein, mouse; D009419:Nerve Tissue Proteins