Continuous treatment process of mercury removal from aqueous solution by growing recombinant E. coli cells and modeling study.
A continuous treatment process was developed to investigate the capability of genetically engineered E. coli to simultaneously accumulate mercuric ions and reproduce itself in a continuous stirred tank reactor (CSTR) system. The influence of dilution rate and initial Hg(2+) concentration on continuous process was evaluated. Results indicated that the recombinant E. coli could effectively accumulate Hg(2+) from aqueous solution with Hg(2+) removal ratio up to about 90%, and propagate its cells at the same time in the continuous treatment system under suitable operational conditions. A kinetic model based on mass balance of Hg(2+) was proposed to simulate the continuous process. The modeling results were in good agreement with the experimental data.
Deng
X
X
Institute of Ecology and Environment, College of Life Science, Shenzhen University, Shenzhen, PR China. dengxu@szu.edu.cn
Hu
Z L
ZL
Yi
X E
XE
D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008628:Mercury / Q000378:metabolism*; D008954:Models, Biological; D030781:Organisms, Genetically Modified; D012996:Solutions; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
D012996:Solutions; D014874:Water Pollutants, Chemical; D008628:Mercury