Addition of deoxynucleosides enhances human immunodeficiency virus type 1 integration and 2LTR formation in resting CD4+ T cells.
Resting CD4+ T cells restrict human immunodeficiency virus (HIV) infection at a step prior to integration. Despite this restriction, we showed previously that HIV integration occurs in resting CD4+ T cells in vitro, albeit at lower levels than in activated CD4+ T cells. Here we show that addition of deoxynucleosides enhanced integration and 2LTR formation in resting CD4+ T cells but that the kinetics were still significantly delayed compared to those of activated T cells. Thus, deoxynucleoside addition partially overcomes the restriction to HIV infection in resting CD4+ T cells.
Plesa
Gabriela
G
Department of Pathology and Laboratory Medicine, Division of Transfusion Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Dai
Jihong
J
Baytop
Cliff
C
Riley
James L
JL
June
Carl H
CH
O'Doherty
Una
U
D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000821:virology*; D004197:Disease Reservoirs; D016325:HIV Long Terminal Repeat / Q000235:genetics / Q000502:physiology; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D009705:Nucleosides / Q000737:chemistry / Q000378:metabolism*; D016662:Virus Integration; D017735:Virus Latency
D009705:Nucleosides