title: Cryogelation for preparation of novel biodegradable tissue-engineering scaffolds.
abstract: 2-Hydroxyethyl methacrylate-L-lactate (HEMA-LLA) and HEMA-L-lactate-dextran (HEMA-LLA-D) were synthesized. 1H-NMR confirmed the formation of these oligomers and macromers. Cryogels with different pore structures were prepared using different amounts of HEMA, HEMA-LLA and HEMA-LLA-D by a cryogelation technique. SEM micrographs exhibited pore morphologies. Cryogels were highly porous with interconnected pore structures, opaque, spongy and highly elastic. It was possible to compress them to remove the water in the pores and to return to their original form just by immersing them in water in few minutes, which was quite reproducible. Their swelling abilities, compressive strengths and degradation in buffer solutions were found to be related with their structural properties which was controlled by changing the cryogelation recipe.
authors.affiliation: Hacettepe University, Chemical Engineering Department and Bioengineering Division, Beytepe, Ankara, Turkey.
authors.forename: Nimet
authors.initials: N
authors.lastname: Bölgen
authors.forename: Fatima
authors.initials: F
authors.lastname: Plieva
authors.forename: Igor Yu
authors.initials: IY
authors.lastname: Galaev
authors.forename: Bo
authors.initials: B
authors.lastname: Mattiasson
authors.forename: Erhan
authors.initials: E
authors.lastname: Pişkin
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D015925:Cryopreservation; D005782:Gels / Q000138:chemical synthesis / Q000737:chemistry*; D007773:Lactates / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D005782:Gels; D007773:Lactates; D008689:Methacrylates; C005044:hydroxyethyl methacrylate