Protection from infection with severe acute respiratory syndrome coronavirus in a Chinese hamster model by equine neutralizing F(ab')2.
To warrant potential clinical testing, the equine anti-severe acute respiratory syndrome coronavirus (SARS-CoV) F(ab')(2) requires evaluation in as many animal models as possible. In this study, we established a new animal model, the Chinese hamster, susceptible to SARS-CoV infection. SARS-CoV could propagate effectively and sustain high levels for 1 wk in animal lungs. All animals were protected from SARS-CoV infection in preventive settings. Further, when used therapeutically this antibody led to an approximately 4-log(10) decrease in viral burden in infected animal lungs. The pathological changes in lungs correlated closely with the dose of antibody administered. The excellent preventive and therapeutic roles of equine anti-SARS-CoV F(ab')(2) in several animal models, including the novel Chinese hamster model described in this study, have provided exciting data concerning its potential clinical study.
Luo
Deyan
D
Department of Immunology, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, People's Republic of China.
Ni
Bing
B
Zhao
Guangyu
G
Jia
Zhengcai
Z
Zhou
Lili
L
Pacal
Marek
M
Zhang
Liangyan
L
Zhang
Songle
S
Xing
Li
L
Lin
Zhihua
Z
Wang
Li
L
Li
Jintao
J
Liang
Yunfei
Y
Shi
Xinfu
X
Zhao
Tingting
T
Zou
Liyun
L
Wu
Yuzhang
Y
Wang
Xiliang
X
D000818:Animals; D000914:Antibodies, Viral / Q000627:therapeutic use*; D006224:Cricetinae; D003412:Cricetulus; D004195:Disease Models, Animal; D005260:Female; D006736:Horses / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000627:therapeutic use*; D008168:Lung / Q000473:pathology / Q000821:virology; D009500:Neutralization Tests; D045473:Severe acute respiratory syndrome-related coronavirus / Q000302:isolation & purification; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology / Q000517:prevention & control*
D000914:Antibodies, Viral; D007140:Immunoglobulin Fab Fragments