title: Stereoisomer analysis of wastewater-derived beta-blockers, selective serotonin re-uptake inhibitors, and salbutamol by high-performance liquid chromatography-tandem mass spectrometry.
abstract: A reversed-phase enantioselective liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) method was developed to measure enantiomer fractions (EF) and concentrations of pharmaceuticals in wastewater. Enantiomer resolution of six beta-blockers (atenolol, metoprolol, nadolol, pindolol, propranolol, and sotalol) along with two selective serotonin re-uptake inhibitors (citalopram, fluoxetine) and one beta(2)-agonist (salbutamol) was achieved with the Chirobiotic V stationary phase. Analyte recovery averaged 86% in influent and 78% in effluent with limits of detection ranging from 0.2 to 7.5 ng/L. These results represent an improvement in wastewater EF measurement for atenolol, metoprolol and propranolol as well as the first EF measurements of citalopram, fluoxetine, nadolol, pindolol, salbutamol and sotalol in wastewaters. Changes in EF through treatment indicate biologically mediated stereoselective processes were likely occurring during wastewater treatment.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.
authors.forename: Sherri L
authors.initials: SL
authors.lastname: MacLeod
authors.forename: Priya
authors.initials: P
authors.lastname: Sudhir
authors.forename: Charles S
authors.initials: CS
authors.lastname: Wong
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000032:analysis* / Q000737:chemistry; D000420:Albuterol / Q000032:analysis* / Q000737:chemistry; D002170:Canada; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017367:Selective Serotonin Reuptake Inhibitors / Q000032:analysis* / Q000737:chemistry; D012722:Sewage / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000295:instrumentation* / Q000379:methods; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: D000319:Adrenergic beta-Antagonists; D000420:Albuterol; D017367:Selective Serotonin Reuptake Inhibitors; D012722:Sewage; D014874:Water Pollutants, Chemical