The biological effects of cell-delivered brain-derived neurotrophic factor on cultured spiral ganglion cells.
The benefit achieved by the use of cochlear implants depends among other factors on the number of surviving spiral ganglion cells (SGCs). Neurotrophic factors, especially brain-derived neurotrophic factor (BDNF), have a protective effect on spiral ganglions. Coating of the cochlear implant electrode with BDNF-producing cells may provide long-term delivery of the factor. Therefore, the hypothesis that BDNF-producing fibroblasts can enhance cell survival of cultured SGCs was tested. Lentiviral infection of fibroblasts resulted in BDNF production. Conditioned medium obtained from infected fibroblasts was used for the cultivation of SGCs. As a result, improved survival and neurite outgrowth was observed on SGCs. Our results demonstrate that lentivirally infected fibroblasts produce BDNF that has neurotrophic effects on spiral ganglions.
Warnecke
Athanasia
A
Department of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.
Wissel
Kirsten
K
Hoffmann
Andrea
A
Hofmann
Nicola
N
Berkingali
Nurdanat
N
Gro
Gerhard
G
Lenarz
Thomas
T
Stöver
Timo
T
D000818:Animals; D000831:Animals, Newborn; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D019929:Cochlear Implantation / Q000379:methods; D017077:Culture Media, Conditioned / Q000378:metabolism / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D005822:Genetic Vectors; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D041681:NIH 3T3 Cells; D016501:Neurites / Q000187:drug effects / Q000648:ultrastructure; D009475:Neurons, Afferent / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013136:Spiral Ganglion / Q000187:drug effects* / Q000378:metabolism; D014162:Transfection
D019208:Brain-Derived Neurotrophic Factor; D017077:Culture Media, Conditioned