title: [Cryopreservation of spermatozoa].
abstract: Sperm cryopreservation has revolutionized the field of assisted reproduction. However, it causes cryodamage to the structure and function of spermatozoa during the freezing-thawing process. Optimization of sperm cryopreservation is necessary for the preservation of male fertility. Cryodamage can be reduced effectively by such methods as improvement of semen quality before freezing, spermatozoal selection, addition of optimal cryoprotectants and application of appropriate thawing techniques. Recent studies focus on cryobiology, improvement of freezing-thawing methods, especially for poor quality semen, and evaluation criteria for post-thaw spermatozoa.
authors.affiliation: Department of Cell Biology, College of Basic Medicine, Jilin University, Changchun, Jilin 130021, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.forename: Rui-zhi
authors.initials: RZ
authors.lastname: Liu
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D006801:Humans; D008297:Male; D012662:Semen Preservation / Q000379:methods*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*