title: The roles of mast cells and Kupffer cells in rat systemic anaphylaxis.
abstract: Mast cells and other cells such as macrophages have been shown to mediate systemic anaphylaxis. We determined the roles of mast cells and Kupffer cells in hepatic and systemic anaphylaxis of rats. Roles of mast cells were examined by using the mast cell-deficient white spotting (Ws/Ws) rat; the Ws/Ws and wild type (+/+) rats were sensitized with ovalbumin (1 mg). Roles of Kupffer cells were examined by depleting Kupffer cells using gadolinium chloride or liposome-encapsulated dichloromethylene diphosphonate in the Ws/Ws and Sprague-Dawley rats. An intravenous injection of 0.6 mg ovalbumin caused substantial anaphylactic hypotension in both the Ws/Ws and +/+ rats; however, the occurrence was delayed in the Ws/Ws rats. After antigen, portal venous pressure increased by 13.1 cmH2O in the +/+ rats, while it increased only by 5.7 cmH2O in the Ws/Ws rats. In response to antigen, the isolated perfused liver of the Ws/Ws rats also showed weak venoconstriction, the magnitude of which was one tenth as large as that of the +/+ rats, indicating that hepatic anaphylaxis was primarily due to mast cells. In contrast, Kupffer cell depletion did not attenuate anaphylactic hepatic venoconstriction in isolated perfused livers. In conclusion, mast cells are involved mainly in anaphylactic hepatic presinusoidal portal venoconstriction but only in the early stage of anaphylactic systemic hypotension in rats. Macrophages, including Kupffer cells, do not participate in rat hepatic anaphylactic venoconstriction.
authors.affiliation: Dept. of Physiology II, Kanazawa Medical Univ., Uchinada 920-0293, Japan. shibamo@kanazawa-med.ac.jp
authors.forename: Toshishige
authors.initials: T
authors.lastname: Shibamoto
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Shimo
authors.forename: Sen
authors.initials: S
authors.lastname: Cui
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Takano
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Kurata
authors.forename: Hideaki
authors.initials: H
authors.lastname: Tsuchida
mesh_terms: D000707:Anaphylaxis / Q000473:pathology* / Q000503:physiopathology*; D000818:Animals; D001783:Blood Flow Velocity; D001794:Blood Pressure; D002478:Cells, Cultured; D006503:Hepatic Veins / Q000503:physiopathology*; D007728:Kupffer Cells; D008102:Liver Circulation; D008297:Male; D008407:Mast Cells; D051381:Rats; D014661:Vasoconstriction