title: Identification of genes differentially expressed during adventitious shoot induction in Pinus pinea cotyledons by subtractive hybridization and quantitative PCR.
abstract: As part of a study aimed at understanding the physiological and molecular mechanisms involved in adventitious shoot bud formation in pine cotyledons, we conducted a transcriptome analysis to identify early-induced genes during the first phases of adventitious caulogenesis in Pinus pinea L. cotyledons cultured in the presence of benzyladenine. A subtractive cDNA library with more than 700 clones was constructed. Of these clones, 393 were sequenced, analyzed and grouped according to their putative function. Quantitative real-time PCR analysis was performed to confirm the differential expression of 30 candidate genes. Results are contrasted with available data for other species.
authors.affiliation: Area de Fisiología Vegetal, Departamento BOS, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, E-33071, Oviedo, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Alonso
authors.forename: Millán
authors.initials: M
authors.lastname: Cortizo
authors.forename: Francisco R
authors.initials: FR
authors.lastname: Cantón
authors.forename: Belén
authors.initials: B
authors.lastname: Fernández
authors.forename: Ana
authors.initials: A
authors.lastname: Rodríguez
authors.forename: Maria L
authors.initials: ML
authors.lastname: Centeno
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Cánovas
authors.forename: Ricardo J
authors.initials: RJ
authors.lastname: Ordás
mesh_terms: D001593:Benzyl Compounds; D018548:Cotyledon / Q000235:genetics* / Q000254:growth & development; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D015723:Gene Library; D007701:Kinetin / Q000494:pharmacology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D028223:Pinus / Q000235:genetics* / Q000254:growth & development; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000235:genetics* / Q000254:growth & development; D011687:Purines; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D046509:Tissue Culture Techniques
chemical_list: D001593:Benzyl Compounds; D018076:DNA, Complementary; D010937:Plant Growth Regulators; D011687:Purines; C480551:benzylaminopurine; D007701:Kinetin