title: Protein changes in the albedo of citrus fruits on postharvesting storage.
abstract: In this work, major protein changes in the albedo of the fruit peel of Murcott tangor (tangerine x sweet orange) during postharvest ageing were studied through 2D PAGE. Protein content in matured on-tree fruits and in fruits stored in nonstressing [99% relative humidity (RH) and 25 degrees C], cold (99% RH and 4 degrees C), and drought (60% RH and 25 degrees C) conditions was initially determined. Protein identification through MS/MS determinations revealed in all samples analyzed the occurrence of manganese superoxide dismutase (Mn SOD), actin, ATP synthase beta subunit (ATPase), citrus salt-stress associated protein (CitSap), ascorbate peroxidase (APX), translationally controlled tumor protein (TCTP), and a cysteine proteinase (CP) of the papain family. The latter protein was identified in two different gel spots, with different molecular mass, suggesting the simultaneous presence of the proteinase precursor and its active form. While Mn SOD, actin, ATPase, and CitSap were unchanged in the assayed conditions, TCTP and APX were downregulated during the postharvest ageing process. Ageing-induced APX repression was also reversed by drought. CP contents in albedo, which were similar in on- and off-tree fruits, were strongly dependent upon cold storage. The active/total CP protein ratio significantly increased after cold exposure. This proteomic survey indicates that major changes in protein content in the albedo of the peel of postharvest stored citrus fruits are apparently related to the activation of programmed cell death (PCD).
authors.affiliation: Research Technology Support Facility, Michigan State University, East Lansing, Michigan 48824, USA.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Lliso
authors.forename: Francisco R
authors.initials: FR
authors.lastname: Tadeo
authors.forename: Brett S
authors.initials: BS
authors.lastname: Phinney
authors.forename: Curtis G
authors.initials: CG
authors.lastname: Wilkerson
authors.forename: Manuel
authors.initials: M
authors.lastname: Talón
mesh_terms: D060387:Ascorbate Peroxidases; D002957:Citrus; D003080:Cold Temperature; D003546:Cysteine Endopeptidases / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005519:Food Preservation / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D010544:Peroxidases / Q000032:analysis; D014674:Plant Proteins, Dietary / Q000032:analysis*
chemical_list: D014674:Plant Proteins, Dietary; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D003546:Cysteine Endopeptidases