Metabolic profiling using Fourier-transform ion-cyclotron-resonance mass spectrometry.
With the aid of the extreme resolving power of Fourier-transform ion-cyclotron-resonance mass spectrometry (FT-ICR/MS), we have developed a metabolomics platform for high-throughput metabolic profiling and metabolite candidate identification integrating a data-processing system, the Dr.DMASS program ( http://kanaya.naist.jp/DrDMASS/ ), and a metabolite-species database, KNApSAcK ( http://kanaya.aist-nara.ac.jp/KNApSAcK/ ). We discuss the potential of this FT-ICR/MS-based metabolic profiling scheme as a general metabolomics tool by clarification of plant metabolic disorders and specific metabolite accumulation patterns caused by herbicidal enzyme inhibitors.
Ohta
Daisaku
D
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai 599-8531, Japan. ohtad@bioinfo.osakafu-u.ac.jp
Shibata
Daisuke
D
Kanaya
Shigehiko
S
D017357:Cyclotrons; D016208:Databases, Factual; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005583:Fourier Analysis; D006540:Herbicides / Q000494:pharmacology; D013058:Mass Spectrometry / Q000379:methods*; D008660:Metabolism; D010944:Plants / Q000378:metabolism; D012984:Software
D004791:Enzyme Inhibitors; D006540:Herbicides