title: The contractile segment of the abneural limbus in the gecko cochlea is enriched in vimentin.
abstract: Previously, we discovered a contractile segment within the cartilaginous abneural limbus of the gecko cochlea, the noncartilaginous abneural limbus (NAL, Ganeshina and Vorobyev, J Comp Neurol 461:539-547, 2003). Here, we demonstrate, by means of SDS-PAGE electrophoresis, the nanoLC-ESI-MSMS technique, immunoblotting, and immunocytochemistry, that the major cytoskeletal protein of the NAL cells is vimentin. Filamentous actin constitutes a minor component of the NAL contractile cell cytoskeleton. Our data indicate that the NAL represents a previously unknown specialization of connective tissue, characterized by the reduction of extracellular matrix and a hypertrophy of the vimentin-based intracellular cytoskeleton. The results are compatible with our hypothesis that the NAL is involved in an adaptation of the cochlear mechanics.
authors.affiliation: School of Biomedical Sciences, University of Queensland, Brisbane, QLD 4072, Australia. o.ganeshina@uq.edu.au
authors.forename: Olga
authors.initials: O
authors.lastname: Ganeshina
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Kuznetsov
authors.forename: Misha
authors.initials: M
authors.lastname: Vorobyev
mesh_terms: D000818:Animals; D003051:Cochlea / Q000033:anatomy & histology / Q000502:physiology / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000378:metabolism; D008116:Lizards / Q000033:anatomy & histology / Q000378:metabolism*; D009119:Muscle Contraction; D014746:Vimentin / Q000032:analysis / Q000378:metabolism*
chemical_list: D014746:Vimentin