Laser-assisted fabrication of biomolecular sensing microarrays.
With the aim of obtaining high density biosensing arrays we use pulsed laser deposition to immobilize functional biomolecules on useful surfaces, and micro- and nanopatterning techniques for fabrication of prototype immunosensing bioarrays. We report biological activity tests demonstrating the functional properties of the immobilized proteins and atomic force microscopy characterization of films of nanometric dimensions. Laser-fabricated immunofluorescent arrays are analyzed to check that the intensity and contrast of the sensing sites allow efficient device fabrication. We have also developed an elementary array of heterogeneous reaction sites and tested its performance by simultaneous incubation with the different specific antigens.
Gagliardi
Serena
S
ENEA C.R. Casaccia Via Anguillarese, 00123 Rome, Italy. serena.gagliardi@casaccia.enea.it
Rapone
Bruno
B
Mosiello
Lucia
L
Luciani
Domenico
D
Gerardino
Annamaria
A
Morales
Piero
P
D001704:Biopolymers / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007834:Lasers; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001704:Biopolymers