title: Preparation and blood coagulation evaluation of chitosan microspheres.
abstract: Cross-linked chitosan microspheres (40-100 microm) with smooth surface were prepared by the methods of emulsification and ethanol coagulant. FTIR results showed that the cross-linking reaction occurred on the amino groups of chitosan molecules. The swelling characteristic of chitosan microspheres was influenced by the environment pH, being generally greater at low rather than higher pH values. The coagulation properties of chitosan microspheres were evaluated by dynamic blood clotting, platelet adhesion and activation, erythrocyte adhesion, hemolysis, and protein absorption assays. Chitosan microspheres can shorten the clotting time and induce the adhesion and activation of platelets. But the shortening of clotting time by chitosan microspheres may be related to not only platelet aggregation, but also erythrocyte aggregation. Take together, chitosan microspheres may be potential use as thrombospheres.
authors.affiliation: College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao 266003, P.R. China.
authors.forename: Qi Zhao
authors.initials: QZ
authors.lastname: Wang
authors.forename: Xi Guang
authors.initials: XG
authors.lastname: Chen
authors.forename: Zi Xiang
authors.initials: ZX
authors.lastname: Li
authors.forename: Song
authors.initials: S
authors.lastname: Wang
authors.forename: Cheng Sheng
authors.initials: CS
authors.lastname: Liu
authors.forename: Xiang Hong
authors.initials: XH
authors.lastname: Meng
authors.forename: Chen Guang
authors.initials: CG
authors.lastname: Liu
authors.forename: Ying Hui
authors.initials: YH
authors.lastname: Lv
authors.forename: Le Jun
authors.initials: le J
authors.lastname: Yu
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D002417:Cattle; D048271:Chitosan / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004903:Erythrocyte Aggregation / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006490:Hemostatics / Q000138:chemical synthesis / Q000494:pharmacology; D066298:In Vitro Techniques; D008863:Microspheres; D010316:Particle Size; D010973:Platelet Adhesiveness / Q000187:drug effects; D011817:Rabbits
chemical_list: D006490:Hemostatics; D048271:Chitosan