Human nonmetastatic clone 23 type 1 gene suppresses migration of cervical cancer cells and enhances the migration inhibition of fungal immunomodulatory protein from Ganoderma tsugae.
The authors investigate the effects of human nonmetastatic clone 23 type 1 (nm23-H1 ) gene and fungal immunomodulatory protein-Ganoderma tsugae (FIP-gts) on the metastatic potential of cervical cancer cells and assess whether nm23-H1 can influence the action of FIP-gts using cell migration and invasion assays and gelatin zymography. The nm23-H1 gene was stably transfected into Caski cells, which lacked nm23-H1 expression. The results show that nm23-H1 stably transfected Caski cells exhibit reduced cell migration but no change of cell invasion and matrix metalloproteinase (MMP)-2 and -9 activities. FIP-gts reduced cell migration in SiHa and nm23-H1 transfected Caski cells more significantly compared with Caski cells and reduced invasion in Caski and nm23-H1-transfected Caski cells, but it exerted no influence on MMP-2 and MMP-9 activities in them. Conclusively, the nm23-H1 gene suppresses cervical cancer cell migration but not invasion and activities of MMP-2 and MMP-9 and enhances the inhibition of FIP-gts upon migration.
Wang
Po-Hui
PH
Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung, Taiwan.
Yang
Shun-Fa
SF
Chen
Gin-Den
GD
Han
Chih-Ping
CP
Chen
Shiuan-Chih
SC
Lin
Long-Yau
LY
Ko
Jiunn-Liang
JL
D045744:Cell Line, Tumor; D002464:Cell Migration Inhibition / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics; D005260:Female; D005656:Fungal Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D032902:Ganoderma / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D007155:Immunologic Factors / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics* / Q000502:physiology; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology*
D005656:Fungal Proteins; D007155:Immunologic Factors; D054778:NM23 Nucleoside Diphosphate Kinases; C517938:NME1 protein, human