title: Involvement of the calcium-dependent proteolytic system in skeletal muscle aging.
abstract: Aging is associated with a progressive and involuntary loss of muscle mass also known as sarcopenia. This condition represents a major public health concern with high socio-economics implications. Although sarcopenia is well documented, the aetiology of this condition still remains poorly understood. Calpains are ubiquitous proteases regulated in part by a specific inhibitor, calpastatin. They are well known to have major implications in muscle growth and differentiation. The aim of the present study was to determine if this proteolytic system could be involved in the phenotype associated with sarcopenia. Calpains and calpastatin levels, subcellular distributions and activities were compared between muscles from 3 and 24 months old rats. Altogether, the results we obtained showed an overall increase in calpain activities associated with muscle aging. These findings suggest that the calcium-dependent proteolytic system is indeed involved in sarcopenia.
authors.affiliation: Université Bordeaux I, INRA USC 2009, Unité Protéolyse Croissance et Développement Musculaire, ISTAB, avenue des facultés, 33405 Talence Cedex, France. e.dargelos@istab.u-bordeaux1.fr
authors.forename: Elise
authors.initials: E
authors.lastname: Dargelos
authors.forename: Cédric
authors.initials: C
authors.lastname: Brulé
authors.forename: Lydie
authors.initials: L
authors.lastname: Combaret
authors.forename: Abdessatar
authors.initials: A
authors.lastname: Hadj-Sassi
authors.forename: Sandrine
authors.initials: S
authors.lastname: Dulong
authors.forename: Sylvie
authors.initials: S
authors.lastname: Poussard
authors.forename: Patrick
authors.initials: P
authors.lastname: Cottin
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000378:metabolism; D002154:Calpain / Q000032:analysis / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D002135:Calcium-Binding Proteins; C033668:calpastatin; D002154:Calpain; D002118:Calcium