Determination of josamycin in rat plasma by capillary electrophoresis coupled with post-column electrochemiluminescence detection.
A novel determination method for josamycin (JOS) based on capillary electrophoresis-electrochemiluminescence detection has been described. In this study, platinum disk electrode (300 microm in diameter) was used as a working electrode and the conditions affecting separation and detection were investigated in detail. Under optimal condition: 40 cm separation capillary (75 microm i.d.); 1.25 V applied potential on the Pt disc of the ECL detector cell; 5 mM Ru(bpy)3(2+) and 50mM phosphate buffer (pH 7.5) in the detection cell; 12 kV separation voltage; 8s injection time; 10 kV injection voltage and 15 mM running buffer (pH 7.5), calibration curve was linear over the range from 10 ng/mL to 5.0 microg/mL with a detection limit of 3.1 ng/mL at a signal-to-noise ratio of 3. The method can be successfully applied for the determination of josamycin in rat plasma in 6 min and the extraction recoveries with spiked plasma samples were over 92%.
Deng
Biyang
B
College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China. dengby16@163.com
Kang
Yanhui
Y
Li
Xianfeng
X
Xu
Qiumei
Q
D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood*; D004563:Electrochemistry / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015570:Josamycin / Q000097:blood*; D008163:Luminescent Measurements / Q000379:methods*; D009942:Organometallic Compounds / Q000737:chemistry; D051381:Rats; D015203:Reproducibility of Results
D000900:Anti-Bacterial Agents; D009942:Organometallic Compounds; C415768:tris(2,2'-bipyridyl)ruthenium(II); D015570:Josamycin