Retrieval of zona-free immature oocytes in a woman with recurrent empty follicle syndrome: a case report.
BACKGROUND
Different ovulation trigger methods such as gonadotropin releasing hormone-agonist (GnRH-a) and recombinant human chorionic gonadotropin (r-hCG) plus rescue oocyte retrieval might reveal oocytes in patients with recurrent empty follicle syndrome.


METHODS
Endogenous luteinizing hormone was triggered with a GnRH-a (Buserelin [Suprefact pro-injection, Aventis-Pharma, Turkey], 250 microg subcutaneously) in a GnRH antagonist (Cetrorelix [Cetrotide 0.25, SeronoTurkey], 0.25 mg/d, starting on day 6), down-regulated cycle. At the first scheduled retrieval, 3 cumulus-oocytecorona complexes were recovered from the left ovary. During chemical denudation with hyaluronidase, 2 of them underwent lysis. The third was a zona-free, germinalvesicle-stage oocyte after mechanical denudation. Oocyte pickup was stopped, and recombinant human chorionic gonadotropin (hCG) (250 microg subcutaneously) was injected. Five cumulus-oocyte-corona complexes were retrieved from the right ovary 24 hours after rescue with recombinant hCG. Only mechanical denudation was done, and 4 zona-free oocytes with germinal vesicle breakdown were seen. All oocytes underwent intracytoplasmic sperm injection, and none of them were fertilized.


CONCLUSIONS
Oocyte maturation defects should be included in etiologic mechanisms for counseling patients with empty follicle syndrome.
Duru
Namik Kemal
NK
Department of Obstetrics and Gynecology, GATA School of Medicine, Ankara, Turkey. nkduru@gata.edu.tr
Cincik
Mehmet
M
Dede
Murat
M
Hasimi
Adnan
A
Baser
Iskender
I
D000328:Adult; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D004334:Drug Administration Schedule; D005260:Female; D005300:Fertility Agents, Female / Q000008:administration & dosage*; D043373:Follicle Stimulating Hormone, Human / Q000008:administration & dosage; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists*; D006801:Humans; D007247:Infertility, Female / Q000188:drug therapy; D007986:Luteinizing Hormone / Q000008:administration & dosage; D009865:Oocytes / Q000187:drug effects; D006080:Ovarian Follicle / Q000002:abnormalities / Q000187:drug effects*; D010062:Ovulation Induction / Q000379:methods*
D006063:Chorionic Gonadotropin; D005300:Fertility Agents, Female; D043373:Follicle Stimulating Hormone, Human; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone