Developments in the use and fabrication of organic monolithic phases for use with high-performance liquid chromatography and capillary electrochromatography.
Capillary electrochromatography suffered in its development because of difficulty in producing stable columns with good permeability. Variability in frit characteristics gave rise to non-reproducible capillaries whose fabrication was extremely difficult and time consuming. Monolithic stationary phases gained popularity in the early 1990s due to the fact that they were easy to fabricate and required no retaining frits. They were also able to be manufactured in a wide variety of chemistries which made them very interesting to the analytical chemist who is constantly looking for materials with different selectivity to the popular silica-based stationary phases.
Smith
Norman W
NW
Micro Separations Group, Pharmaceutical Science Research Division King's College, London, UK. norman.2.smith@kcl.ac.uk
Jiang
Zhengjin
Z
D000178:Acrylamides / Q000737:chemistry; D000596:Amino Acids / Q000302:isolation & purification; D053801:Capillary Electrochromatography / Q000295:instrumentation*; D002241:Carbohydrates / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D057927:Hydrophobic and Hydrophilic Interactions; D007474:Ion Exchange; D009696:Nucleic Acids / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification; D004364:Pharmaceutical Preparations / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification; D013237:Stereoisomerism; D014815:Vitamins / Q000302:isolation & purification
D000178:Acrylamides; D000596:Amino Acids; D002241:Carbohydrates; D009696:Nucleic Acids; D010455:Peptides; D004364:Pharmaceutical Preparations; D011506:Proteins; D014815:Vitamins