Nicotianamine preferentially inhibits Angiotensin I-converting enzyme.
Nicotianamine (NA) is a nonprotein amino acid that inhibits the angiotensin I-converting enzyme (ACE) in the renin-angiotensin system (RAS). The purpose of this study is to prove that NA contributes to the suppression of hypertension by preferential inhibition of ACE. On comparison with EDTA-a chelator-we found that the inhibition pattern of NA for ACE is that of mixed inhibition and that NA exhibits weak chelation effects for zinc, copper, and cobalt ions. Therefore, we investigated whether NA inhibited zinc-containing enzymes other than ACE in vitro. The results revealed that NA does not inhibit leucine aminopeptidase or alkaline phosphatase in rat serum. On the other hand, NA demonstrated specific inhibitory effects for rat serum ACE and aortic ACE. These results suggest that the preferential inhibition of circulatory and tissue ACE by NA can contribute to the suppression of hypertension.
Hayashi
Atsumi
A
Department of Food and Nutrition, Tokyo Kasei University, Tokyo, Japan. ahayashi@tokyo-kasei.ac.jp
Kimoto
Koichi
K
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D001383:Azetidinecarboxylic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology*; D003035:Cobalt / Q000378:metabolism; D003300:Copper / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D006973:Hypertension / Q000188:drug therapy* / Q000201:enzymology; D020128:Inhibitory Concentration 50; D007931:Leucyl Aminopeptidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D012084:Renin-Angiotensin System / Q000187:drug effects; D015032:Zinc / Q000378:metabolism
D000806:Angiotensin-Converting Enzyme Inhibitors; D002614:Chelating Agents; C082893:nicotianamine; D003035:Cobalt; D001383:Azetidinecarboxylic Acid; D003300:Copper; D004492:Edetic Acid; D000469:Alkaline Phosphatase; D007931:Leucyl Aminopeptidase; D007703:Peptidyl-Dipeptidase A; D015032:Zinc