Role of pigment epithelium-derived factor on proliferation and migration of choroidal capillary endothelium induced by vascular endothelial growth factor in vitro.
BACKGROUND
Pigment epithelium-derived factor (PEDF) is expressed in several normal organs and identified as an inhibitor of neovascularization. In the present study, we investigated the effect of PEDF in an in vitro model of ocular choroidal neovascularization.


METHODS
Microdissection was used to isolate the human choroidal endothelial cells (CECs), followed by the use of superparamagnetic beads (Dynabeads) coated with the CD31 antibody, which selectively binds to the endothelial cell surface. The mitogenic and motogenic effects of vascular endothelial growth factor (VEGF) on cultured choroidal capillary endothelial cells were examined in the presence or absence of PEDF (1, 10, 100, and 1000 ng/ml) using cell counts and migration assays.


RESULTS
Cells bound to the beads were isolated using a magnetic particle concentrator and they were successfully cultured and characterized to be endothelial cells that possessed greater than 95% immunoreactivity to von Willebrand factor. PEDF suppressed the proliferation and migration of VEGF-induced choroidal capillary endothelial cells. However, the concentration of PEDF which we used has little effect on normal CECs.


CONCLUSIONS
PEDF played an important role on the growth and migration of VEGF-stimulated choroidal endothelial cell. These findings suggest that PEDF may be an effective approach to the treatment of choroidal neovascular disorders.
Wang
Feng-hua
FH
Department of Ophthalmology, Affiliated First People's Hospital of Shanghai Jiao Tong University, Shanghai 200080, China.
Sun
Xiao-dong
XD
Zhang
Xi
X
Xu
Xun
X
Zhu
Qi
Q
Huang
Jian-nan
JN
Fan
Ying
Y
Gu
Qing
Q
Liu
Hai-yang
HY
D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002829:Choroid / Q000098:blood supply* / Q000187:drug effects; D020256:Choroidal Neovascularization / Q000188:drug therapy; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D005136:Eye Proteins / Q000494:pharmacology*; D006801:Humans; D009414:Nerve Growth Factors / Q000494:pharmacology*; D015843:Serpins / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*; D000099174:Pigment Epithelium-Derived Factor
D005136:Eye Proteins; D009414:Nerve Growth Factors; D015843:Serpins; D042461:Vascular Endothelial Growth Factor A; D000099174:Pigment Epithelium-Derived Factor