Detection of pim-1 mRNA in prostate cancer diagnosis.
BACKGROUND
Pim-1 plays an important role in the apoptosis, proliferation, differentiation of cancer cells and progression of cancer. In this study we detected the expression of pim-1 mRNA in normal prostate, benign prostatic hyperplasia (BPH), and prostate cancer (PCa) and explored its diagnostic value for PCa.


METHODS
The prostate tissues were collected from 23 patients with PCa, 37 patients with BPH, and 3 healthy volunteers. Pim-1 mRNA expression levels in these samples were determined by the quantitative real-time PCR (QRT-PCR). The differences of expression were calculated based on a standard curve.


RESULTS
The ratio of pim-1 mRNA to beta-actin in the normal prostate, BPH, and PCa were 1.05 +/- 0.04, 2.57 +/- 0.74 and 4.45 +/-0.63, respectively. The differences among PCa, BPH and NT were significant (P < 0.05, respectively).


CONCLUSIONS
Detecting pim-1 mRNA expression by QRT-PCR provides a reliable metric for the diagnosis of PCa.
He
Hui-chan
HC
Department of Urology, First People's Hospital of Guangzhou, Guangzhou Medical College, Guangzhou 510180, China.
Bi
Xue-cheng
XC
Dai
Qi-shan
QS
Wang
Shao-sheng
SS
Wei
Hong-ai
HA
Zhong
Wei-de
WD
Liu
Wen-hua
WH
Jiang
Fu-neng
FN
Liu
Liang-shi
LS
D000368:Aged; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000378:metabolism; D011470:Prostatic Hyperplasia / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D051573:Proto-Oncogene Proteins c-pim-1 / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D012680:Sensitivity and Specificity
D012333:RNA, Messenger; C496634:PIM1 protein, human; D051573:Proto-Oncogene Proteins c-pim-1