title: In silico identification and mapping of microsatellite markers on sus scrofa chromosome 4.
abstract: Marker density of a QTL region on pig chromosome 4 was increased. New microsatellites were identified by in silico mining of BAC-end and genomic shotgun sequences. Among 8,784 BAC-end sequences predicted within the region, 148 microsatellites were identified. In addition, 27,450 CA/TG repeats were identified within the genomic shotgun sequences, of which 157 were most likely located on SSC4q. A selection of 61 new microsatellites was mapped, together with previously mapped markers. The results showed that the human-pig comparative map in combination with BAC-end and genomic sequence resources provides an excellent source for a highly efficient and targeted development of markers.
authors.affiliation: Institute for Pig Genetics, Beuningen, The Netherlands. Rik.vanWijk@wur.nl
authors.forename: H J
authors.initials: HJ
authors.lastname: van Wijk
authors.forename: B
authors.initials: B
authors.lastname: Harlizius
authors.forename: S C
authors.initials: SC
authors.lastname: Liefers
authors.forename: H
authors.initials: H
authors.lastname: Buschbell
authors.forename: B
authors.initials: B
authors.lastname: Dibbits
authors.forename: M A M
authors.initials: MA
authors.lastname: Groenen
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D033481:Chromosomes, Mammalian; D019295:Computational Biology; D018895:Microsatellite Repeats; D040641:Quantitative Trait Loci; D013552:Swine / Q000235:genetics*