[Correlation of 53BP1 and p53 polymorphisms to susceptibility to esophageal squamous cell carcinoma and gastric cardiac adenocarcinoma].
OBJECTIVE
53BP1 is one of p53-binding proteins, which can enhance the transcriptional activation of p53 and plays a key role in tumor suppression. A single nucleotide polymorphism (SNP) T885G has been found in the promoter of 53BP1. This study was to investigate the correlation of 53BP1 and p53 SNPs to susceptibility to esophageal squamous cell carcinoma (ESCC) and gastric cardiac adenocarcinoma (GCA) in a high incidence area of Hebei Province in China.


METHODS
Genotypes of 53BP1 T885G and p53 Arg72Pro in 349 ESCC patients, 275 GCA patients, and 635 healthy subjects were detected by primer-introduced restriction analysis-polymerase chain reaction (PIRA-PCR).


RESULTS
The overall distribution of 53BP1 T885G was not significantly different between ESCC patients, GCA patients and healthy subjects (P>0.05). When stratified by smoking status and family history of upper gastrointestinal cancer (UGIC), the distribution of 53BP1 T885G was not significantly different between ESCC patients, GCA patients and healthy subjects. Compared with p53 Arg72Pro Arg/Arg genotype, Pro/Pro genotype decreased the susceptibility to GCA [the age, sex, smoking status, and family history adjusted odds ratio (OR)=0.79, 95% confidence interval (CI)=0.64-0.98]. Stratification analysis showed that Pro/Pro genotype decreased the susceptibility to GCA among non-smokers (adjusted OR=0.72, 95% CI=0.54-0.97), but p53 Arg72Pro had no influence on the susceptibility to ESCC. Stratified by p53 Arg72Pro genotype, 53BP1 T885G G/G genotype reduced the susceptibility to GCA among the individuals with Pro allele (Arg/Pro and Pro/Pro genotypes) (adjusted OR=0.74, 95% CI=0.57-0.95).


CONCLUSIONS
53BP1 T885G may not be correlated to the susceptibility to ESCC and GCA in the high incidence area of Hebei Province in China; p53 Arg72Pro Pro/Pro genotype could decrease the susceptibility to GCA; 53BP1 T885G G/G genotype could reduce the susceptibility to GCA among the individuals with p53 Pro allele.
Cao
Yan-Yan
YY
Laboratory of Molecular Biology, Hebei Provincial Cancer Institute/The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, Hebei, 050011, P. R. China.
Ge
Hui
H
Chen
Long-Qi
LQ
Chen
Zhi-Feng
ZF
Wen
Deng-Gui
DG
Li
Yan
Y
Zhang
Jian-Hui
JH
D000230:Adenocarcinoma / Q000453:epidemiology / Q000235:genetics / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000235:genetics / Q000378:metabolism; D002299:Cardia; D002681:China / Q000453:epidemiology; D004938:Esophageal Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism; D000071857:Tumor Suppressor p53-Binding Protein 1
D047908:Intracellular Signaling Peptides and Proteins; C498301:TP53BP1 protein, human; D016159:Tumor Suppressor Protein p53; D000071857:Tumor Suppressor p53-Binding Protein 1