title: Human immunodeficiency virus type 1 Nef protein modulates the lipid composition of virions and host cell membrane microdomains.
abstract: BACKGROUND
  The Nef protein of Human Immunodeficiency Viruses optimizes viral spread in the infected host by manipulating cellular transport and signal transduction machineries. Nef also boosts the infectivity of HIV particles by an unknown mechanism. Recent studies suggested a correlation between the association of Nef with lipid raft microdomains and its positive effects on virion infectivity. Furthermore, the lipidome analysis of HIV-1 particles revealed a marked enrichment of classical raft lipids and thus identified HIV-1 virions as an example for naturally occurring membrane microdomains. Since Nef modulates the protein composition and function of membrane microdomains we tested here if Nef also has the propensity to alter microdomain lipid composition.
  
  
  RESULTS
  Quantitative mass spectrometric lipidome analysis of highly purified HIV-1 particles revealed that the presence of Nef during virus production from T lymphocytes enforced their raft character via a significant reduction of polyunsaturated phosphatidylcholine species and a specific enrichment of sphingomyelin. In contrast, Nef did not significantly affect virion levels of phosphoglycerolipids or cholesterol. The observed alterations in virion lipid composition were insufficient to mediate Nef's effect on particle infectivity and Nef augmented virion infectivity independently of whether virus entry was targeted to or excluded from membrane microdomains. However, altered lipid compositions similar to those observed in virions were also detected in detergent-resistant membrane preparations of virus producing cells.
  
  
  CONCLUSIONS
  Nef alters not only the proteome but also the lipid composition of host cell microdomains. This novel activity represents a previously unrecognized mechanism by which Nef could manipulate HIV-1 target cells to facilitate virus propagation in vivo.
authors.affiliation: Heidelberg University Biochemistry Center (BZH), Heidelberg, Germany. britta.bruegger@bzh.uni-heidelberg.de
authors.forename: Britta
authors.initials: B
authors.lastname: Brügger
authors.forename: Ellen
authors.initials: E
authors.lastname: Krautkrämer
authors.forename: Nadine
authors.initials: N
authors.lastname: Tibroni
authors.forename: Claudia E
authors.initials: CE
authors.lastname: Munte
authors.forename: Susanne
authors.initials: S
authors.lastname: Rauch
authors.forename: Iris
authors.initials: I
authors.lastname: Leibrecht
authors.forename: Bärbel
authors.initials: B
authors.lastname: Glass
authors.forename: Sebastian
authors.initials: S
authors.lastname: Breuer
authors.forename: Matthias
authors.initials: M
authors.lastname: Geyer
authors.forename: Hans-Georg
authors.initials: HG
authors.lastname: Kräusslich
authors.forename: Hans Robert
authors.initials: HR
authors.lastname: Kalbitzer
authors.forename: Felix T
authors.initials: FT
authors.lastname: Wieland
authors.forename: Oliver T
authors.initials: OT
authors.lastname: Fackler
mesh_terms: D015704:CD4 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D015683:Gene Products, gag / Q000378:metabolism; D015497:HIV-1 / Q000378:metabolism* / Q000472:pathogenicity; D006801:Humans; D021962:Membrane Microdomains / Q000378:metabolism* / Q000821:virology; D010713:Phosphatidylcholines / Q000378:metabolism; D013109:Sphingomyelins / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology; D014771:Virion / Q000302:isolation & purification / Q000378:metabolism* / Q000472:pathogenicity; D014779:Virus Replication / Q000502:physiology; D054311:nef Gene Products, Human Immunodeficiency Virus / Q000378:metabolism*
chemical_list: D015704:CD4 Antigens; D015683:Gene Products, gag; D010713:Phosphatidylcholines; D013109:Sphingomyelins; D054311:nef Gene Products, Human Immunodeficiency Virus; C514837:nef protein, Human immunodeficiency virus 1; C029449:polyene phosphatidylcholine; D002784:Cholesterol