[Transplantation and magnetic resonance imaging of transplantable human glioma tissue in the brain of nude mice].
OBJECTIVE
In previous reports, orthotopic transplantation models of glioma were produced by injecting cell suspension into the brain of mice, which is complex, time-consuming, and nearly impossible to prepare in a large scale within a short period. This study was to establish human glioma orthotopic transplantation model in nude mice by transplanting tumor tissue in the brain, and investigate magnetic resonance imaging (MRI) of the transplanted tumors.


METHODS
Human glioma cells were injected subcutaneously into nude mice to form human glioma. The transplantable human glioma tissues (2 mm3) were put into trocar and directly injected into the caudate nucleus of nude mice. Thirty days later, the tumors were detected by 1.5T superconduct MR machine with micro-23 coil and measured by Dicomworks (V1.35) software. Tumor morphology was observed under light microscope with HE staining. Tumor volume was measured under stereomicroscope. The feasibility of measuring tumor volume according to MRI data was evaluated.


RESULTS
MRI showed that in the 15 nude mice received orthotopic transplantation in the caudate nucleus, 14 developed glioma. Under microscope, glioma tissues were found at the same sites as where MRI indicated. Tumor volume was (23.45+/-11.64) mm3 as measured by MRI and (23.19+/-10.18) mm3 as detected under stereomicroscope (P>0.05). The successful rate of tumor model preparation was 93% (14/15). The successful rate of tumor imaging by MRI was 100% (14/14).


CONCLUSIONS
Tissue quantitative transplantation via trocar is simple, time-saving, and easy to construct tumor model in a large scale with high successful rate. The 1.5T MR machine with micro-23 coil can be used to observe tumor position and size of orthotopic transplantation models of human glioma in nude mice.
Li
Ru-Jun
RJ
Department of Neurosurgery, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu, 215004, PR China.
Diao
Yi
Y
Huang
Qiang
Q
Shen
Jun-Kang
JK
Lan
Qing
Q
D000818:Animals; D001921:Brain / Q000473:pathology; D001932:Brain Neoplasms / Q000473:pathology*; D002421:Caudate Nucleus / Q000473:pathology; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005910:Glioma / Q000473:pathology*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D047368:Tumor Burden