title: [Creation of tissue engineered cartilage with internal support].
abstract: OBJECTIVE
  To test the hypothesis that tissue-engineered cartilage can be bioincorporated with a nonreactive, permanent endoskeletal scaffold.
  
  
  METHODS
  Chondrocytes obtained from swine articular were seeded onto polyglycolic acids(PGA) scaffold which was incorporated with high-density polyethylene (Medpor). After cultured in vitro for two weeks,the cell-scaffold construct was implanted into subcutaneous pockets on the back of nude mice. Six weeks later,the newly formed cartilage prosthesis was harvested, and a small part of sample was evaluated by gross view, histology, type II collagen immunohistochemistry and biochemistry. PGA scaffold seeded with cells as the control group.
  
  
  RESULTS
  The newly formed cartilage was very similar to normal cartilage in both gross view and histology, and jointed Medpor tightly. The center of control group was hollow.
  
  
  CONCLUSIONS
  This pilot technique combining tissue engineering with a permanent success in creating cartilage without "hollow" phenomenon. biocompatible endoskeleton demonstrated
authors.affiliation: Department of Plastic Surgery, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China.
authors.forename: Yu-jia
authors.initials: YJ
authors.lastname: Wu
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Guang-dong
authors.initials: GD
authors.lastname: Zhou
authors.forename: Wan-yao
authors.initials: WY
authors.lastname: Xia
authors.forename: Xu-song
authors.initials: XS
authors.lastname: Luo
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002356:Cartilage / Q000637:transplantation*; D019902:Chondrocytes / Q000166:cytology; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010865:Pilot Projects; D011095:Polyethylenes; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; C084562:Medpor; D011095:Polyethylenes