Study on olfactory function in GABAC receptor/channel rho1 subunit knockout mice.
The GABAC receptor/channel rho1 subunit plays an important role in the inhibitory pathway and sensory processing in the retina and spinal cord. Although it was suggested that the rho1 subunit plays a role in olfactory sensations, the precise role of the rho1 subunit in olfactory sensory function is still not clear. In the present study, we report that olfactory function was significantly altered in rho1 subunit knockout (rho1-/-) mice compared to its wildtype counterpart. The rho1 subunit mRNA, detected by reverse transcription (RT)-PCR experiments, was expressed in the olfactory bulb of wild-type mice. Expression of rho1 subunit proteins in the olfactory bulb was detected by immunohistochemistry in mitral cells in the mitral cell layer. Neither mRNA nor proteins of the rho1 subunit were found in olfactory bulb neurons in rho1-/- mice. Alterations of olfactory function in rho1-/- mutant mice compared to their wildtype littermates were examined by olfactory behavioral test. We found that sensitivity to the smell of citral odorant in rho1-/- mice was significantly greater compared to that of wildtype mice. Our results indicate that the GABAC rho1 subunit acts in olfactory bulb neurons as an inhibitory modulator that affects the process of olfactory signaling transmission.
Chen
Yan
Y
Department of Medical Genetics, Shanghai Jiaotong University School of Medicine, 280 Chongqing Nan Rd., Shanghai 200025, PR China.
Zhou
Dongping
D
Zhou
Kun
K
Ren
Yiping
Y
Dai
Wei
W
Xu
Ming
M
Lu
Luo
L
Lu
Zhenyu
Z
D000818:Animals; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009433:Neural Inhibition / Q000235:genetics; D009474:Neurons / Q000378:metabolism*; D009830:Olfactory Bulb / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D018079:Receptors, GABA / Q000235:genetics*; D011963:Receptors, GABA-A; D012684:Sensory Thresholds / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics; D012903:Smell / Q000235:genetics*
C087200:GABA-C receptor; C528239:Gabrr1 protein, rat; D021122:Protein Subunits; D012333:RNA, Messenger; D018079:Receptors, GABA; D011963:Receptors, GABA-A