Noninvasive monitoring of radiation-induced treatment response using proton magnetic resonance spectroscopy and diffusion-weighted magnetic resonance imaging in a colorectal tumor model.
OBJECTIVE
To examine whether in vivo proton magnetic resonance spectroscopy ((1)H MRS) and diffusion-weighted magnetic resonance imaging (DW-MRI) can monitor radiation-induced changes in HT29 xenografts in mice.


METHODS
HT29 xenografts in mice received a dose of 15Gy. In vivo(1)H MRS and DW-MRI were acquired pretreatment and 1, 3, 6 and 10 days post-irradiation. After imaging, tumors were excised for histological analysis. The amounts of necrosis, fibrosis and viable cells in the cross sections were scored and compared to changes in apparent diffusion coefficient (ADC) and choline/water ratio.


RESULTS
Radiation-induced necrosis in the xenografts was observed as increased tumor ADC. In-growth of fibrosis three days post-irradiation restricting water mobility was accompanied by decreased tumor ADC. Choline/water ratio correlated with metabolic activity and tumor growth.


CONCLUSIONS
ADC and choline/water ratio assessed by in vivo DW-MRI and (1)H MRS depicts radiation-induced changes in HT29 xenografts following irradiation.
Seierstad
Therese
T
Department of Medical Physics, Rikshospitalet-Radiumhospitalet Medical Center, Oslo, Norway. therese@radium.uio.no
Røe
Kathrine
K
Olsen
Dag Rune
DR
D000818:Animals; D002794:Choline / Q000032:analysis; D015179:Colorectal Neoplasms / Q000473:pathology / Q000532:radiotherapy*; D038524:Diffusion Magnetic Resonance Imaging; D004195:Disease Models, Animal; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D014867:Water / Q000032:analysis
D014867:Water; D002794:Choline