title: Skewed association of polyfunctional antigen-specific CD8 T cell populations with HLA-B genotype.
abstract: We studied CD8 T cell responses against HIV-1, cytomegalovirus, Epstein-Barr virus, and influenza in 128 subjects and demonstrate that polyfunctional CD8 T cell responses, also including IL-2 production and Ag-specific proliferation, are predominantly driven by virus epitopes restricted by HLA-B alleles. Interestingly, these protective CD8 T cells are equipped with low-avidity T cell receptors (TCRs) for the cognate virus epitope. Conversely, HLA-A-restricted epitopes are mostly associated with "only effector" IFN-gamma-secreting, with cytotoxicity, and with the lack of IL-2 production and Ag-specific proliferation. These CD8 T cells are equipped with high-avidity TCR and express higher levels of the T cell exhaustion marker PD-1. Thus, the functional profile of the CD8 T cell response is strongly influenced by the extent to which there is stimulation of polyfunctional (predominantly restricted by HLA-B) versus only effector (restricted by HLA-A) T cell responses. These results provide the rationale for the observed protective role of HLA-B in HIV-1-infection and new insights into the relationship between TCR avidity, PD-1 expression, and the functional profile of CD8 T cells.
authors.affiliation: Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Harari
authors.forename: Cristina
authors.initials: C
authors.lastname: Cellerai
authors.forename: Felicitas
authors.initials: F
authors.lastname: Bellutti Enders
authors.forename: Josef
authors.initials: J
authors.lastname: Köstler
authors.forename: Laura
authors.initials: L
authors.lastname: Codarri
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Tapia
authors.forename: Onur
authors.initials: O
authors.lastname: Boyman
authors.forename: Erika
authors.initials: E
authors.lastname: Castro
authors.forename: Silvana
authors.initials: S
authors.lastname: Gaudieri
authors.forename: Ian
authors.initials: I
authors.lastname: James
authors.forename: Mina
authors.initials: M
authors.lastname: John
authors.forename: Ralf
authors.initials: R
authors.lastname: Wagner
authors.forename: Simon
authors.initials: S
authors.lastname: Mallal
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pantaleo
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D015331:Cohort Studies; D003587:Cytomegalovirus / Q000276:immunology; D005838:Genotype; D015658:HIV Infections / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology; D015234:HLA-A Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D009975:Orthomyxoviridae / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens; C508594:IL2 protein, human; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell