title: The monolayer formation of Bergmann glial cells is regulated by Notch/RBP-J signaling.
abstract: The Bergmann glia is a unipolar astrocyte in the cerebellar cortex, displaying a tight association with Purkinje cells. The cell bodies of Bergmann glia are located in a row around Purkinje cell somata; they extend radially arranged Bergmann fibers which enwrap the synapses on the Purkinje cell dendrites. It is well known that Bergmann glial somata migrate from the ventricular zone through the mantle zone, forming an epithelium-like lining in the Purkinje cell layer during development. However, the mechanism of the monolayer formation of Bergmann glia is poorly understood. Several reports have suggested that Notch signaling plays instructive roles in promoting the identities of several types of glial cells, including Bergmann glia. Moreover, Notch receptors are expressed in Bergmann glia during development. Here, we have deleted the Notch1, Notch2 and RBP-J genes in the Bergmann glia by GFAP-driven, Cre-mediated recombination, to study the role of Notch-RBP-J-signaling in the monolayer formation of Bergmann glia. Notch1/2- and RBP-J-conditional mutant mice showed disorganization of Bergmann fibers, irregularities of the Bergmann glial lining and aberrant localization of Bergmann glia in the molecular layer. Thus, Notch-RBP-J signaling plays crucial roles in the monolayer formation and morphogenesis of Bergmann glia.
authors.affiliation: Laboratory of Molecular Neuroscience, School of Biomedical Science and Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
authors.forename: Okiru
authors.initials: O
authors.lastname: Komine
authors.forename: Mai
authors.initials: M
authors.lastname: Nagaoka
authors.forename: Kei
authors.initials: K
authors.lastname: Watase
authors.forename: David H
authors.initials: DH
authors.lastname: Gutmann
authors.forename: Kenji
authors.initials: K
authors.lastname: Tanigaki
authors.forename: Tasuku
authors.initials: T
authors.lastname: Honjo
authors.forename: Freddy
authors.initials: F
authors.lastname: Radtke
authors.forename: Toshiki
authors.initials: T
authors.lastname: Saito
authors.forename: Shigeru
authors.initials: S
authors.lastname: Chiba
authors.forename: Kohichi
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D002531:Cerebellum / Q000166:cytology / Q000196:embryology*; D017353:Gene Deletion; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D051881:Receptor, Notch1 / Q000235:genetics / Q000378:metabolism*; D051883:Receptor, Notch2 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; C497762:Notch1 protein, mouse; C497765:Notch2 protein, mouse; C497571:Rbpj protein, mouse; D051881:Receptor, Notch1; D051883:Receptor, Notch2