Cutinase production by Fusarium oxysporum in liquid medium using central composite design.
The objective of the present study was to measure the production of cutinase by Fusarium oxysporum in the presence of several carbon and nitrogen sources (glycides, fatty acids and oils, and several organic and inorganic nitrogen sources), trying to find a cost-effective substitute for cutin in the culture medium as an inducer of cutinase production. The results were evaluated by the Tukey test, and flaxseed oil was found to give the best results as a cutinase inducer. The authors optimized the composition of the growth medium employing response surface methodology. The experimental results were fitted to a second-order polynomial model at a 95% level of significance (p < 0.05). The greatest cutinolytic activity was obtained in a liquid mineral medium supplemented with flaxseed oil, showing an increase in enzymatic activity from 11 to 22.68 U/mL after 48 h of fermentation. A CCD study of the fermentation conditions was carried out, and the best production of cutinase was registered with the use of 30 degrees C and 100 rpm. These results support the use of flaxseed oil as a substitute for cutin, which is difficult and expensive to obtain, for the production of cutinase in a larger scale.
Pio
Tatiana Fontes
TF
Food Science Department, Faculty of Food Engineering, Campinas State University (UNICAMP), P.O. Box 6121, CEP 13083-862, Campinas, SP, Brazil. tatipi@fea.unicamp.br
Macedo
Gabriela Alves
GA
D002244:Carbon / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D003470:Culture Media; D005670:Fusarium / Q000201:enzymology* / Q000254:growth & development; D007218:Industrial Microbiology / Q000379:methods*; D008043:Linseed Oil; D009584:Nitrogen / Q000378:metabolism; D012107:Research Design; D013696:Temperature
D003470:Culture Media; D002244:Carbon; D008043:Linseed Oil; D002265:Carboxylic Ester Hydrolases; C019952:cutinase; D009584:Nitrogen