title: The effects of toxicological agents on the optics and mitochondria of the lens and the mitochondria of the corneal epithelium.
abstract: This review describes how the morphology and distribution of the mitochondria of the epithelium and the superficial fibre layers of the lens were studied using confocal scanning laser microscopy. This research was correlated with an effort to use the optical properties of the intact lens in culture as a proxy for the cornea in measuring ocular toxicity. In turn, this work led to the confocal study of the in vitro and then the in vivo cornea and their possible use in using confocal microscopy to evaluate the effect of various treatments on the integrity of the surface of the eye. Finally, confocal examination of the mitochondria of the lens has provided an avenue to the study of mitochondrial dynamics.
authors.affiliation: School of Optometry, University of Waterloo, West Waterloo, ON, Canada.
authors.forename: V
authors.initials: V
authors.lastname: Bantseev
authors.forename: D J
authors.initials: DJ
authors.lastname: McCanna
authors.forename: J-Y
authors.initials: JY
authors.lastname: Driot
authors.forename: J G
authors.initials: JG
authors.lastname: Sivak
mesh_terms: D000438:Alcohols / Q000633:toxicity*; D000818:Animals; D003315:Cornea / Q000033:anatomy & histology / Q000648:ultrastructure; D019573:Epithelium, Corneal / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D007908:Lens, Crystalline / Q000033:anatomy & histology / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D008928:Mitochondria / Q000187:drug effects*; D055095:Optics and Photonics; D009924:Organ Culture Techniques; D013501:Surface-Active Agents / Q000633:toxicity*; D018675:Toxicity Tests / Q000379:methods; D014116:Toxicology / Q000379:methods
chemical_list: D000438:Alcohols; D013501:Surface-Active Agents