Successful vaccination against Leishmania chagasi infection in BALB/c mice with freeze-thawed Leishmania antigen and Corynebacterium parvum.
This study evaluated the potential of a Leishmania antigen vaccine in protecting BALB/c mice against Leishmania chagasi. Mice received two subcutaneous doses of L. amazonensis vaccine with Corynebacterium parvum and subsequent boost was done without adjuvant. One week later, mice were challenged with L. chagasi. We observed that this vaccine caused a significant reduction in parasite load in liver and spleen and induced a high production of IFN-gamma and IL-4 by spleen cells from vaccinated mice in response to Leishmania antigen. Together, our data show that this vaccine is capable of inducing a Th1/Th2 response that is important to control parasite replication.
Vilela
Márcia de Carvalho
Mde C
Laboratório de Imunoparasitologia, DECBI/NUPEB, Campus Universitário, Morro do Cruzeiro, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
Gomes
Daniel Cláudio de Oliveira
DC
Marques-da-Silva
Eduardo de Almeida
Ede A
Serafim
Tiago Donatelli
TD
Afonso
Luis Carlos Crocco
LC
Rezende
Simone Aparecida
SA
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D007891:Leishmania / Q000276:immunology*; D007896:Leishmaniasis / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D054332:Leishmaniasis Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000627:therapeutic use; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D011425:Propionibacterium acnes / Q000276:immunology*; D013154:Spleen / Q000187:drug effects / Q000378:metabolism / Q000469:parasitology; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology
D000953:Antigens, Protozoan; D054332:Leishmaniasis Vaccines; D015847:Interleukin-4; D007371:Interferon-gamma