title: Behaviors of keratinocytes and fibroblasts on films of PLA50-PEO-PLA50 triblock copolymers with various PLA segment lengths.
abstract: The growth of human primary keratinocytes and fibroblasts on PLA-PEO-PLA copolymer films was investigated as an intermediate stage of a strategy aimed at making implantable dermo-epidermal substitutes. Four PLA-PEO-PLA triblock copolymers with the same PEO block and different DL-lactic acid/ethylene oxide molar ratios (LA/EO) (0.8, 1.4, 1.8 and 2), were synthesized and characterized by 1H-nuclear magnetic resonance and infrared spectroscopy. The films made of these copolymers were more hydrophilic than PLA50 and than tissue culture polystyrene controls according to contact angles with water. Proliferation and adhesion of human skin cells were evaluated by MTT assay and by scanning electron microscopy. The presence of PEO in the triblock copolymers influenced cell adhesion and proliferation of fibroblasts, whereas keratinocyte adhesion and proliferation were not affected. These features emphasize the interest of PLA-PEO-PLA triblock copolymers to serve as better compounds than the racemic PLA previously investigated to make supports for human skin primary cells and scaffolds for skin engineering.
authors.affiliation: Faculty of Pharmacy, CRBA, UMR CNRS 5473, University Montpellier 1, 15 Avenue Charles Flahault, Montpellier Cedex 5 34093, France. xavier.garric@univ-montp1.fr
authors.forename: Xavier
authors.initials: X
authors.lastname: Garric
authors.forename: Henri
authors.initials: H
authors.lastname: Garreau
authors.forename: Michel
authors.initials: M
authors.lastname: Vert
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Molès
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D005027:Ethylene Oxide / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D019344:Lactic Acid / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D012867:Skin / Q000473:pathology; D013055:Spectrophotometry, Infrared; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods
chemical_list: D003470:Culture Media; D011091:Polyesters; D011108:Polymers; D011137:Polystyrenes; D013778:Tetrazolium Salts; D013844:Thiazoles; C507889:polylactide-polyethylene glycol-polylactide; D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide); C022616:thiazolyl blue; D005027:Ethylene Oxide