title: Polymerase chain reaction of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia in primary endodontic infections.
abstract: The aim of this study was to investigate the correlation between endodontic clinical signs and symptoms and the presence of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia or their association by nested polymerase chain reaction assay. Microbial samples were taken from 50 cases with necrotic pulp tissues in primary infections. DNA was extracted from the samples, which were analyzed for the presence of three endodontic pathogens by using species-specific primers. P gingivalis, T denticola, and T forsythia were detected in 46%, 38%, and 22% of the symptomatic cases, respectively. The bacterial complex composed by T forsythia, P gingivalis, and T denticola was found in 14% of the cases with spontaneous pain, tenderness to percussion, swelling, and pain on palpation. The high prevalence of P gingivalis, T denticola, and T forsythia in the samples examined suggests that these bacteria are related to the etiology of symptomatic periradicular diseases.
authors.affiliation: Department of Restorative Dentistry, Dental School of Piracicaba, State University of Campinas, Avenida Limeira 901-Vila Areião, 13414-903 Piracicaba, São Paulo, Brazil. bpgomes@fop.unicamp.b0072
authors.forename: Brenda P F A
authors.initials: BP
authors.lastname: Gomes
authors.forename: Francisco
authors.initials: F
authors.lastname: Montagner
authors.forename: Rogério Castilho
authors.initials: RC
authors.lastname: Jacinto
authors.forename: Alexandre A
authors.initials: AA
authors.lastname: Zaia
authors.forename: Caio Cezar Randi
authors.initials: CC
authors.lastname: Ferraz
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Souza-Filho
mesh_terms: D016009:Chi-Square Distribution; D004269:DNA, Bacterial / Q000032:analysis*; D003786:Dental Pulp Cavity / Q000000981:diagnostic imaging / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000302:isolation & purification*; D011671:Pulpitis / Q000382:microbiology; D011859:Radiography; D014098:Toothache / Q000000981:diagnostic imaging / Q000382:microbiology*; D048373:Treponema denticola / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial