Fusion and molecular aspects of liposomal nanocarriers incorporated with isoprenoids.
The present study was conducted to investigate whether typical isoprenyl compounds (TICs) can control liposomal fusion reactions through changes in the physical properties of membranes. The fusion capabilities of TIC-incorporated liposomes were characterized by measuring the 13C spin-lattice relaxation times (13CT1) and the gel permeation chromatogram (GPC) patterns. The 13CT1 relaxivities of some of these TIC-liposomes were remarkably enhanced at 27 degrees C. The highest 13CT1 value obtained was for the beta-carotene-liposome, which ruptured, and was attributed to the highest membrane fusion reactivity. The other TIC-liposomes incorporated with alpha-tocopherol, canthaxanthin, or coenzyme Q10 also induced significant fusion and did not rupture in comparison with the beta-carotene-liposome. These results show that the incorporations of TICs into lipid bilayers are useful to control liposomal nanocarriers for suitable membrane packing and advantageous phase separation, which could affect membrane-related processes.
Nakagawa
Hidenori
H
Department of Biomedical Engineering, Graduate School of Medicine, University of Tokyo, Tokyo 113-0033, Japan. hnakagawa@medes.m.u-tokyo.ac.jp
Shiina
Tsuyoshi
T
Sekino
Masaki
M
Kotani
Makoto
M
Ueno
Shoogo
S
D004337:Drug Carriers / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008560:Membrane Fluidity; D008561:Membrane Fusion; D008968:Molecular Conformation; D010316:Particle Size; D013729:Terpenes / Q000737:chemistry*
D004337:Drug Carriers; D008051:Lipid Bilayers; D008081:Liposomes; D013729:Terpenes