Physiological characterization and identification of genes differentially expressed in response to drought induced by PEG 6000 in Populus canadensis leaves.
We report here about the physiological and molecular responses of Populus canadensis (clone Dorskamp) to drought. The stress was applied to young rooted cuttings by PEG 6000 application over 30 days. This stress induces a decrease in predawn leaf water potential. After 10 days of stress, there was a decrease in stomatal conductance and a slight retardation of leaf growth, but the osmotic potential remained constant. Using the differential display technique, we searched for genes differentially expressed in response to drought at this date. Thirty-six differentially expressed leaf cDNAs were detected between stressed and control conditions. Thirty-four cDNAs clones were successfully cloned and 23 were found to share high identity with Arabidopsis thaliana and Populus trichocarpa genes. The transcriptional regulation of 21 genes was examined by reverse RNA dot blot, confirming an increase in expression for 16 of them after 10 days of treatment. Among these 16 genes, most of them are involved in a different cellular metabolic pathway. These differentially expressed genes are also involved and/or regulated by other treatments such as salt, withholding water or auxin application. The maintenance of growth observed during the first 10 days of the stress period could be due to the regulation of these genes and can be a common response between herbaceous plants and trees.
Caruso
Aurore
A
Laboratoire de Physiologie et Biochimie Végétale, EA-2663, Université du Maine, Faculté des Sciences et Techniques, Avenue Olivier Messiaen, 72085 Le Mans Cedex 9, France.
Chefdor
Françoise
F
Carpin
Sabine
S
Depierreux
Christiane
C
Delmotte
Francis M
FM
Kahlem
Guy
G
Morabito
Domenico
D
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics; D002999:Clone Cells; D055864:Droughts; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant; D009995:Osmosis / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D054046:Plant Stomata / Q000187:drug effects / Q000502:physiology; D011092:Polyethylene Glycols / Q000494:pharmacology*; D032107:Populus / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D018749:RNA, Plant / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013997:Time Factors; D014867:Water
D018749:RNA, Plant; D014867:Water; D011092:Polyethylene Glycols