title: Paenibacillus humicus sp. nov., isolated from poultry litter compost.
abstract: Two bacterial strains, PC-142 and PC-147(T), isolated from poultry litter compost, were characterized with respect to their phenetic and phylogenetic characteristics. The isolates were endospore-forming rods that were reddish in colour after Gram staining. They were catalase- and oxidase-positive, were able to degrade starch and gelatin and grew at 15-40 degrees C and pH 5.5-10.0. The predominant fatty acids were anteiso-C(15 : 0), iso-C(15 : 0) and iso-C(16 : 0), the major respiratory quinone was menaquinone MK-7, the cell-wall peptidoglycan was of the A1gamma type and the G+C content of the DNA was 58 mol%. The 16S rRNA gene sequence analysis and phenetic characterization indicated that these organisms belong to the genus Paenibacillus, with Paenibacillus pasadenensis SAFN-007(T) as the closest phylogenetic neighbour (97.5 %). Strains PC-142, PC-147(T) and P. pasadenensis SAFN-007(T) represent a novel lineage within the genus Paenibacillus, characterized by a high DNA G+C content (58-63 mol%). The low levels of 16S rRNA gene sequence similarity with respect to other taxa with validly published names and the identification of distinctive phenetic features in the two isolates indicate that strains PC-142 and PC-147(T) represent a novel species of the genus Paenibacillus, for which the name Paenibacillus humicus sp. nov. is proposed. The type strain is PC-147(T) (=DSM 18784(T) =NBRC 102415(T) =LMG 23886(T)).
authors.affiliation: Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4200-072 Porto, Portugal.
authors.forename: Ivone
authors.initials: I
authors.lastname: Vaz-Moreira
authors.affiliation: Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4200-072 Porto, Portugal.
authors.forename: Cátia
authors.initials: C
authors.lastname: Faria
authors.affiliation: Departamento de Zoologia, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: M Fernanda
authors.initials: MF
authors.lastname: Nobre
authors.affiliation: DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7b, D-38124 Braunschweig, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: LEPAE - Departamento de Engenharia Química, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto, Portugal.
authors.forename: Olga C
authors.initials: OC
authors.lastname: Nunes
authors.affiliation: Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4200-072 Porto, Portugal.
authors.forename: Célia M
authors.initials: CM
authors.lastname: Manaia
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000096:biosynthesis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000382:microbiology; D005780:Gelatin / Q000378:metabolism; D020459:Genes, rRNA; D016936:Gram-Positive Endospore-Forming Bacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000096:biosynthesis; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D011200:Poultry / Q000382:microbiology*; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013213:Starch / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D005780:Gelatin; D013213:Starch; D010088:Oxidoreductases; D002374:Catalase