title: Cyclin G associated kinase interacts with interleukin 12 receptor beta2 and suppresses interleukin 12 induced IFN-gamma production.
abstract: Interleukin 12 receptor beta1 (IL-12Rbeta1) and beta2 (IL-12Rbeta2) constitute the functional and high-affinity receptor complex for interleukin 12 (IL-12) and mediate important functions in activated T cells. In this study, we identified cyclin G associated kinase (GAK) as a new IL-12Rbeta2-interacting protein using yeast two-hybrid system and confirmed it by coimmunoprecipitation assays. Overexpression of GAK in activated T cells suppresses IL-12 induced IFN-gamma production but has no detectable effects on its proliferation, whereas knockdown of GAK by RNA interference (RNAi) increases IFN-gamma production. These results suggest that GAK associates with IL-12Rbeta2 and may play a role in regulating IL-12 signaling.
authors.affiliation: Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
authors.forename: Yu Jie
authors.initials: YJ
authors.lastname: Tang
authors.forename: Hong Liang
authors.initials: HL
authors.lastname: Zong
authors.forename: Jian Xin
authors.initials: JX
authors.lastname: Gu
authors.forename: Wei Wen
authors.initials: WW
authors.lastname: Deng
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Sun
mesh_terms: D000818:Animals; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism*; D018664:Interleukin-12 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D053712:Interleukin-12 Receptor beta 2 Subunit / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D034741:RNA, Small Interfering / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*; D020798:Two-Hybrid System Techniques
chemical_list: C534703:Ccng1 protein, mouse; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins; D053712:Interleukin-12 Receptor beta 2 Subunit; D034741:RNA, Small Interfering; D018664:Interleukin-12; D007371:Interferon-gamma