Inhibition of intestinal tumorigenesis in Apc(min/+) mice by green tea polyphenols (polyphenon E) and individual catechins.
In this work, we compared the cancer preventive activities of Polyphenon E (PPE), a standardized green tea polyphenol preparation given in diet versus drinking fluid as well as the activities of PPE versus individual catechins. We treated Apc(Min/+) mice for 9 wk with 0.08% (-)-epigallocatechin-3-gallate (EGCG), 0.08% (-)-epicatechin-3-gallate, or 0.12% PPE in drinking fluid or diet. Only 0.12% dietary PPE and 0.08% EGCG in drinking fluid significantly decreased tumor multiplicity (70% and 51%, respectively). Compared to PPE in drinking fluid, dietary PPE delivered twofold more EGCG to the small intestine. Immunohistochemistry showed that adenomas in groups treated with PPE and EGCG had decreased cell proliferation, Beta -catenin nuclear expression, and phospho-Akt levels; higher cleaved caspase-3 levels, and partially restored retinoid X receptor alpha expression. The results suggest that these molecular events contribute to the cancer prevention activity of EGCG and PPE. Furthermore, diet appears to be a better route of administration for PPE than drinking fluid.
Hao
Xingpei
X
Department of Chemical Biology, Ernest Mario School of Pharmacy Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
Sun
Yuhai
Y
Yang
Chung S
CS
Bose
Mousumi
M
Lambert
Joshua D
JD
Ju
Jihyeung
J
Lu
Gang
G
Lee
Mao-Jung
MJ
Park
Sunny
S
Husain
Ali
A
Wang
Steven
S
D000704:Analysis of Variance; D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use; D001628:Beverages; D001682:Biological Availability; D002392:Catechin / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000627:therapeutic use; D002455:Cell Division / Q000187:drug effects; D004032:Diet; D004333:Drug Administration Routes; D005260:Female; D017491:Genes, APC; D007150:Immunohistochemistry; D007414:Intestinal Neoplasms / Q000188:drug therapy*; D007421:Intestine, Small / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051057:Proto-Oncogene Proteins c-akt; D013662:Tea / Q000737:chemistry*
D016588:Anticarcinogenic Agents; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate; D051057:Proto-Oncogene Proteins c-akt; C472086:polyphenon E