title: Chemoprotective effect of Spirulina (Arthrospira) against cyclophosphamide-induced mutagenicity in mice.
abstract: The aim of this study was to investigate the antimutagenic effects of Spirulina (SP) on male and female mice by the dominant lethal test using cyclophosphamide (CP) as a mutagen. Animals of both sex were given SP orally at 0, 200, 400 or 800 mg/kg body weight (b.w.) for 2 weeks prior to starting the CP treatment. CP was i.p. injected daily for 5 days at 40 mg/kg b.w. For the male-dominant lethal test, each male was caged with untreated females per week for 3 weeks. For the female-dominant lethal test the above doses and schedule treatments were used and treated females were caged for one week with untreated males (1-2). On days 13-15 after breeding was |started all the females were evaluated for incidence of pregnancy, total corpora lutea, total implants and pre- and post-implant losses. In the male-dominant lethal test, the CP induced pre- and post-implant losses in untreated females were inhibited at all SP doses. In the female-dominant lethal test only post-implantation losses were prevented at the same doses. Semen examination of a separate group of mice showed that SP improved its quality. Our results illustrate protective effects of SP in relation to CP-induced genetic damage to germ cells.
authors.affiliation: Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación Carpio y Plan de Ayala S/N, 11340 México D.F., México. gchamcev@yahoo.com.mx
authors.forename: G
authors.initials: G
authors.lastname: Chamorro-Cevallos
authors.forename: L
authors.initials: L
authors.lastname: Garduño-Siciliano
authors.forename: B L
authors.initials: BL
authors.lastname: Barrón
authors.forename: E
authors.initials: E
authors.lastname: Madrigal-Bujaidar
authors.forename: D E
authors.initials: DE
authors.lastname: Cruz-Vega
authors.forename: N
authors.initials: N
authors.lastname: Pages
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000627:therapeutic use*; D003520:Cyclophosphamide / Q000037:antagonists & inhibitors* / Q000633:toxicity; D005260:Female; D005799:Genes, Dominant / Q000187:drug effects; D008297:Male; D051379:Mice; D009153:Mutagens / Q000633:toxicity*; D011247:Pregnancy / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*; D053545:Spirulina
chemical_list: D016587:Antimutagenic Agents; D009153:Mutagens; D003520:Cyclophosphamide