Reactive blue 19 decolouration by laccase immobilized on silica beads.
Laccase (31.5 U of activity/g or 4.39 mug of protein/m(2)) from Trametes versicolor was immobilized on controlled-porosity-carrier silica beads and evaluated for the decolouration of Reactive blue 19, an anthraquinone dye. Although there was an initial, rapid adsorption of the dye to the packed bed in a recirculating reactor, about 97.5% of Reactive blue 19 removal was due to enzymatic degradation. The free enzyme lost 52% of its activity in 48 h. However, the activity of the immobilized laccase was unchanged after 4 months of storage in phosphate buffer under ambient conditions followed by three successive decolourations over 120 h. Treating the laccase immobilized beads with ethanolamine reduced dye adsorption by 40%.
Champagne
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Chemical Engineering, Queen's University, Kingston, Ontario, K7L 3N6, Canada.
Ramsay
J A
JA
D000880:Anthraquinones / Q000302:isolation & purification* / Q000378:metabolism; D001487:Basidiomycota / Q000201:enzymology*; D003116:Color; D004800:Enzymes, Immobilized / Q000737:chemistry*; D007218:Industrial Microbiology; D042845:Laccase / Q000096:biosynthesis / Q000378:metabolism*; D012822:Silicon Dioxide / Q000737:chemistry
D000880:Anthraquinones; D004800:Enzymes, Immobilized; D012822:Silicon Dioxide; D042845:Laccase; C024758:Remazol Brilliant Blue R