The special location of p-H3 and p-CENP-A on heterochromatin during mitosis in MCF-7.
CENP-A locates at nucleosome as histone H3-like proteins, and is phosphorylated during mitosis. We investigated the dynamic distribution of p-CENP-A to explore the details of its function. We found that p-CENP-A was phosphorylated at late prophase, and the signal of p-CENP-A arranged at equatorial plate along with nucleosomes at metaphase, but moved to midbody at later phase of mitosis. The phosphorylation modification of CENP-A shares some characters of H3, but has different temporal patterns during mitosis. Our results suggested that the CENP-A might have similar functions as H3, but with different patterns for their different binging materials.
Li
Dengwen
D
Department of Biochemistry and Molecular Biology, NanKai University, Tianjin, 300071, China.
Liu
Ruming
R
Song
Liping
L
Zhou
Hao
H
Chen
Jiatong
J
Huang
Xitai
X
D000818:Animals; D001324:Autoantigens / Q000032:analysis* / Q000378:metabolism; D016466:CHO Cells; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis* / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D005455:Fluorescent Antibody Technique; D006570:Heterochromatin / Q000378:metabolism*; D006657:Histones / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008677:Metaphase; D018613:Microscopy, Confocal; D008938:Mitosis; D010766:Phosphorylation; D012694:Serine / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
D001324:Autoantigens; C000619700:CENPA protein, human; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone; D006570:Heterochromatin; D006657:Histones; D012694:Serine