title: Antiphytoviral activity of bruceine-D from Brucea javanica seeds.
abstract: BACKGROUND
  Brucea javanica (L.) Merr. is widely distributed throughout the southern parts of China and has been used in traditional medicine to treat a variety of diseases. The objective of the present study was to identify the active antiphytoviral compound in the seeds of B. javanica and evaluate the inhibitory activity of the compound against plant virus.
  
  
  RESULTS
  Bioassay-guided fractionation of the most active extract from the seeds led to the isolation of an antiphytoviral compound which was identified as bruceine-D by conventional spectroscopy methods. The compound exhibited significant inhibitory activity against the infection and replication of tobacco mosaic virus (TMV), with IC(50) values of 13.98 and 7.13 mg L(-1) respectively. The compound also showed a strong inhibitory effect on the infectivity of potato virus Y (PVY) and cucumber mosaic virus (CMV). Furthermore, the compound could effectively inhibit systemic TMV infection in the host tobacco plant under glasshouse conditions.
  
  
  CONCLUSIONS
  The results suggested that bruceine-D from Brucea javanica may have the potential to be used as a natural viricide, or a lead compound for new viricides.
authors.affiliation: Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Shen
authors.forename: Zheng-Kun
authors.initials: ZK
authors.lastname: Zhang
authors.forename: Zu-Jian
authors.initials: ZJ
authors.lastname: Wu
authors.forename: Ming-An
authors.initials: MA
authors.lastname: Ouyang
authors.forename: Lian-Hui
authors.initials: LH
authors.lastname: Xie
authors.forename: Qi-Ying
authors.initials: QY
authors.lastname: Lin
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry* / Q000494:pharmacology*; D032269:Brucea / Q000737:chemistry*; D015394:Molecular Structure; D010935:Plant Diseases / Q000821:virology; D036702:Quassins / Q000737:chemistry* / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D014026:Nicotiana / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D036702:Quassins; C030412:bruceine D