[A dual effect on the growth of astrocytes of lipopolysaccharide].
OBJECTIVE
To investigate the changes of growth of astrocytes administrated with lipopolysaccharide (LPS) and the probable mechanisms.


METHODS
LPS was administrated to rat astrocytes for 1 h, 6 h, 24 h and 48 h, and the changes of growth of astrocytes were detected by MTT method. Meanwhile, the long-term changes of growth of astrocytes after administration with LPS for 24 h were also studied. Moreover, the effects of inhibitor of NF-kappaB(SN50) on proliferation of astrocytes were observed.


RESULTS
Changes of astrocytes growth were observed only when LPS had been administrated for 24 h. LPS of low concentration could promote proliferation of astrocytes and increase the cell viability(P<0.05 vs control), while LPS of high concentration inhibited the proliferation of astrocytes. Being administrated for 24 h, LPS could promote the proliferation of astrocytes in short-term. As for the long-term effects, LPS inhibited the proliferation of astrocytes in a concentration-dependent manner. Pretreatment with SN50 could block the effects of LPS on astrocytes.


CONCLUSIONS
LPS of low concentration could promote the proliferation of astrocytes in short-term and LPS of high concentration could inhibit it. The molecular mechanism of the changes might be related to the activation of NF-kappaB.
Li
Xue-Zhong
XZ
Department of Neurology, the Second Affiliated Hospital, Soochow University, Suzhou, China. sudalxz@163.com
Zhou
Yuan
Y
Liu
Chun-Feng
CF
Bai
Long-Mei
LM
D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D008070:Lipopolysaccharides / Q000494:pharmacology*; D013997:Time Factors
D008070:Lipopolysaccharides