Transferrin reverses the anti-invasive activity of human prostate cancer cells that overexpress sema3E.
In vitro invasion and adhesion of stably semaphorin (sema) 3E-transfected PC-3 prostate cancer cells were determined in the presence and absence of transferrin. Invasion and adhesion decreased compared to untransfected cells; however, transferrin reversed the effects. Transferrin differentially regulated E-cadherin and beta-catenin in these cells. Insulin growth factor 3 (IGFBP3) negated the invasive and adhesive effects of transferrin. Transferrin increased binding of insulin growth factor (IGF)-1 to the activated IGF-1 receptor, and IGF-1 mimicked the invasive and adhesive effects of transferrin. These data suggest that transferrin modulates sema3E-transfected cells through an IGFBP3/IGF-1-dependent pathway, in part, by regulation of adhesion proteins.
Herman
Jeffery G
JG
The Pharmacology and Toxicology Graduate Program and the Cancer Prevention and Research Center, College of Pharmacy, Washington State University, Pullman, WA 99164-6713, USA.
Meadows
Gary G
GG
D015820:Cadherins / Q000235:genetics; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D018972:Insulin-Like Growth Factor Binding Protein 3; D018969:Insulin-Like Growth Factor Binding Proteins / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D008297:Male; D009361:Neoplasm Invasiveness; D011471:Prostatic Neoplasms / Q000473:pathology*; D039961:Semaphorins / Q000502:physiology*; D014162:Transfection; D014168:Transferrin / Q000494:pharmacology*; D051176:beta Catenin / Q000235:genetics
D015820:Cadherins; C515497:IGFBP3 protein, human; D018972:Insulin-Like Growth Factor Binding Protein 3; D018969:Insulin-Like Growth Factor Binding Proteins; C494123:SEMA3E protein, human; D039961:Semaphorins; D014168:Transferrin; D051176:beta Catenin; D007334:Insulin-Like Growth Factor I