Identification of twinfilin-2 as a factor involved in neurite outgrowth by RNAi-based screen.
Using RNA interference (RNAi) to suppress gene expression, we attempted to identify tyrosine kinases involved in the extension of neurites from SH-SY5Y cells. A comprehensive analysis of gene "knock-down" profiles with small interfering RNAs (siRNAs) revealed candidate proteins that might control neurite extension. Phenotype-based screening of differentiating SH-SY5Y cells following retinoic acid (RA) stimulation indicated that twinfilin-2 is a protein that is involved in neurite outgrowth, as confirmed by morphological analysis of twinfilin-2-overexpressing cells.
Yamada
Shigeru
S
Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Aomi 2-41-6, Kohtoh-ku, Tokyo 135-0064, Japan.
Uchimura
Eiichiro
E
Ueda
Takanori
T
Nomura
Takashi
T
Fujita
Satoshi
S
Matsumoto
Kayo
K
Funeriu
Daniel P
DP
Miyake
Masato
M
Miyake
Jun
J
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation; D006801:Humans; D008840:Microfilament Proteins; D016501:Neurites / Q000187:drug effects / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D051381:Rats; D014212:Tretinoin / Q000494:pharmacology
D002352:Carrier Proteins; D008840:Microfilament Proteins; C120744:TWF2 protein, human; D014212:Tretinoin; D011505:Protein-Tyrosine Kinases