Effects of urocortin, corticotropin-releasing factor (CRF) receptor agonist, and astressin, CRF receptor antagonist, on the sleep-wake pattern: analysis by radiotelemetry in conscious rats.
Stress has been known to release corticotropin-releasing factor (CRF) and have an affect on sleep-wake patterns. However, there is no direct evidence of CRF receptor agonist and antagonist on sleep-wake patterns. Therefore, this study aimed to clarify this point by using radiotelemetry system in conscious rats. Wake, non-rapid eye-moving (NREM) sleep and rapid eye-moving (REM) sleep were analyzed by computer software, simultaneously measuring electroencephalogram and electromyogram. In the light period, urocortin (CRF receptor agonist: i.v.) significantly increased wake duration, and decreased NREM sleep duration. REM sleep was not affected. Astressin (CRF receptor antagonist: i.p.) significantly attenuated the changes induced by urocortin, although astressin itself did not affect the sleep-wake pattern in the light period at this dosage. These findings show that urocortin changes the sleep-wake pattern in the light period. Moreover, urocortin was found to change the sleep-wake pattern by acting on CRF receptor, as astressin significantly attenuated the urocortin-induced changes on sleep-wake patterns.
Uchida
Masayuki
M
Food Science Institute, Division of Research and Development, Meiji Dairies Corporation, Odawara, Kanagawa, Japan. MASAYUKI_UCHIDA@MEIJI-MILK.COM
Suzuki
Masayuki
M
Shimizu
Kimiko
K
D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D003624:Darkness; D003627:Data Interpretation, Statistical; D004567:Electrodes, Implanted; D004569:Electroencephalography / Q000187:drug effects; D004576:Electromyography; D008027:Light; D008297:Male; D018696:Neuroprotective Agents / Q000633:toxicity*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018019:Receptors, Corticotropin-Releasing Hormone / Q000819:agonists* / Q000037:antagonists & inhibitors*; D012890:Sleep / Q000187:drug effects*; D012895:Sleep, REM / Q000187:drug effects; D013686:Telemetry; D054832:Urocortins; D014851:Wakefulness / Q000187:drug effects*
D018696:Neuroprotective Agents; D010446:Peptide Fragments; D018019:Receptors, Corticotropin-Releasing Hormone; D054832:Urocortins; C104416:astressin; D003346:Corticotropin-Releasing Hormone