Genetic and immunological characterization of the 14-3-3xi molecule from Schistosoma bovis.
Currently available candidate vaccines against schistosomiasis elicit only partial protection. In addition, the type of immune response that could lead to the highest level of protection against schistosomes has not yet been described. Thus, efforts should be made in both the identification of novel proteins essential for the parasite cycle and in the modulation of immune responses against these novel candidates through the combined use of immunomodulatory molecules. Several parasites have 14-3-3 proteins, and these proteins are known to play a key role in parasite biology. In the present work, we report the isolation and characterization of a new 14-3-3 gene from Schistosoma bovis and offer new information regarding the genetic structure of the gene. In addition, we have produced the corresponding recombinant protein. Finally, we describe the immune responses elicited by this protein when combined with 4 different immunomodulators in immunized mice.
Uribe
N
N
Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Salamanca, Avda. Campo Charro, s/n, 37007-Salamanca, Spain.
Muro
A
A
Vieira
C
C
Lopez-Aban
J
J
del Olmo
E
E
Suárez
L
L
Martínez-Fernández
A R
AR
Siles-Lucas
M
M
D048948:14-3-3 Proteins / Q000235:genetics* / Q000276:immunology*; D000276:Adjuvants, Immunologic; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry; D005260:Female; D015801:Helminth Proteins / Q000235:genetics* / Q000276:immunology*; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012547:Schistosoma / Q000235:genetics* / Q000276:immunology*; D012552:Schistosomiasis / Q000517:prevention & control; D016415:Sequence Alignment; D014612:Vaccines / Q000235:genetics / Q000276:immunology
D048948:14-3-3 Proteins; D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D011994:Recombinant Proteins; D014612:Vaccines