Capillary electrochromatography without external pressure assistance. Use of packed columns with a monolithic inlet frit.
A fused silica capillary column was packed with RP(18) silica stationary phase entrapping the particles between two frits obtained by two different procedures. The inlet frit consisted of a short organic polymer made via a thermopolymerization process while the outlet frit was prepared by sintering the octadecylsilica (ODS) material. The packed column was employed in capillary electrochromatography (CEC) experiments for the separation of three selected test compounds. Retention time and separation efficiency were evaluated. Results were compared with those ones obtained with a packed capillary containing the same stationary phase entrapped between two sinterized frits. The novel packed column exhibited comparable separation efficiency and resolution with the traditional one. However, it allowed experiments without pressure support during the runs with no bubble formation.
Rocco
Anna
A
Institute of Chemical Methodologies, Consiglio Nazionale delle Ricerche, Area della Ricerca di Roma I, Via Salaria Km 29300, 00016 Monterotondo Scalo, Rome, Italy.
Fanali
Salvatore
S
D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry
D012822:Silicon Dioxide