title: Continuous treatment process of mercury removal from aqueous solution by growing recombinant E. coli cells and modeling study.
abstract: A continuous treatment process was developed to investigate the capability of genetically engineered E. coli to simultaneously accumulate mercuric ions and reproduce itself in a continuous stirred tank reactor (CSTR) system. The influence of dilution rate and initial Hg(2+) concentration on continuous process was evaluated. Results indicated that the recombinant E. coli could effectively accumulate Hg(2+) from aqueous solution with Hg(2+) removal ratio up to about 90%, and propagate its cells at the same time in the continuous treatment system under suitable operational conditions. A kinetic model based on mass balance of Hg(2+) was proposed to simulate the continuous process. The modeling results were in good agreement with the experimental data.
authors.affiliation: Institute of Ecology and Environment, College of Life Science, Shenzhen University, Shenzhen, PR China. dengxu@szu.edu.cn
authors.forename: X
authors.initials: X
authors.lastname: Deng
authors.forename: Z L
authors.initials: ZL
authors.lastname: Hu
authors.forename: X E
authors.initials: XE
authors.lastname: Yi
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008628:Mercury / Q000378:metabolism*; D008954:Models, Biological; D030781:Organisms, Genetically Modified; D012996:Solutions; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D012996:Solutions; D014874:Water Pollutants, Chemical; D008628:Mercury