[Screening for metronidazole-resistance associated gene fragments of Helicobacter pylori by suppression subtractive hybridization].
To screen metronidazole (MTZ) -resistance associated gene fragments of Helicobacter pylori (H.pylori) by suppression subtractive hybridization(SSH).



The suppression subtractive hybridization (SSH) was used to screen the different DNA fragments between MTZ-resistant and -susceptible clinical strains of H.pylori. The resistant strains specific gene fragments were obtained by SSH and identified by dot blotting.



Among the 120 subtractive colonies which were randomly chosen, 37 DNA fragments were different (>or=2 times) in DNA-copy numbers between resistant and susceptible strains and 17 of them were significantly different (>or=3 times). These 17 DNA fragments were sequenced subsequently. Ten of them were new sequences and the other 7 were duplicated sequences. These sequences represented respectively: depeptide ABC transporter periplasmic dipeptide-binding protein (dppA), permease protein (dppB), et al.



Gene fragments specific to MTZ-resistant H. pylori strains were obtained by SSH and these genes may associated with MTZ-resistance of H.pylori.
Li
Ai-qing
AQ
Department of Digestive Diseases, Sir Run Run Shaw Affiliated Hospital, College of Medcine, Zhejiang University, Hangzhou 310016, China.
Dai
Ning
N
Yan
Jie
J
Zhu
Yong-liang
YL
D000890:Anti-Infective Agents / Q000494:pharmacology; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016480:Helicobacter pylori / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D008795:Metronidazole / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D017422:Sequence Analysis, DNA
D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D008795:Metronidazole