title: A new approach for mapping sialylated N-glycosites in serum proteomes.
abstract: A new approach for proteome-wide analysis of sialylated N-glycopeptides based on the diagonal chromatographic COFRADIC technology is presented here. The use of alpha(2-3,6,8,9) neuraminidase is central to isolate sialylated N-glycopeptides out of a complex peptide mixture. Two different COFRADIC techniques are introduced here, either without or with post-metabolic oxygen-18 labeling (direct versus indirect sorting), and when applied to immuno-depleted mouse serum, we herewith identified 93 sialylated glycosylation sites in 53 serum proteins.
authors.affiliation: Department of Medical Protein Research, VIB, B-9000 Ghent, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Ghesquière
authors.forename: Lien
authors.initials: L
authors.lastname: Buyl
authors.forename: Hans
authors.initials: H
authors.lastname: Demol
authors.forename: Jozef
authors.initials: J
authors.lastname: Van Damme
authors.forename: An
authors.initials: A
authors.lastname: Staes
authors.forename: Evy
authors.initials: E
authors.lastname: Timmerman
authors.forename: Joël
authors.initials: J
authors.lastname: Vandekerckhove
authors.forename: Kris
authors.initials: K
authors.lastname: Gevaert
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006020:Glycopeptides / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000737:chemistry; D010103:Oxygen Isotopes / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012794:Sialic Acids / Q000737:chemistry*; D012984:Software
chemical_list: D001798:Blood Proteins; D006020:Glycopeptides; D010103:Oxygen Isotopes; D012794:Sialic Acids; D009439:Neuraminidase