Hysteroscopy and butyl-cyanoacrylate on experimental sterilization of rabbit uterine tubes.
OBJECTIVE
To assess the sterilization effectiveness on uterine tube of rabbit by the cyanoacrylate adhesive.


METHODS
Hysteroscopy tubal catheterization was performed randomly in 12 animals (24 uterine tubes) assigned to the sham group (GS) and 15 animals (30 uterine tubes) to the n-butyl-cyanoacrylate (GB). The female rabbits were observed during 30, 90 and 180 days and mated to fertile males. The no pregnant rabbits were submitted to in vitro burst pressure test for patency by air insufflation (40 mmHg). The microscopic assessment was performed to parameters of damages in epithelium caused by the adhesive, the degree of inflammatory process, morphometry data values of tube diameter (UT) (cm), mucosa thickness (MT) and the myosalpinx thickness (MyT) (mm). The mucosa cells densitometry (total optical density) was expressed by the amount of DNA. The significance of the differences in histological scores and in thickness measurements were made by ANOVA test (P value < 0.05).


RESULTS
In all animals of GB: the adhesive was attached to the mucosa; there was no pregnancy; no records of significant degree on inflammatory process; the patency test was negative and densitometry of DNA showed similar values to the both groups independently of observation periods. The layers thickness of GB-UT(1.118 +/- 0.117), GB-MT(0.447+/-0.247) and GB-MyT(0.853+/-0.097) were larger than the GS-UT(0.666+/-0.409), GS-MT(0.211+/-0.070) and GS-MyT(0.442+/-0.143).


CONCLUSIONS
This approach offers a safe and feasible method of uterine tube obstruction.
Rivoire
Henri Chaplin
HC
Department of Surgery, Head Rio Grande Federal Foundation University, Rio Grande, RS, Brazil. henri@mikrus.com.br
Fagundes
Djalma José
DJ
Bigolin
Sergio
S
Fagundes
Anna Tereza Negrini
AT
D000399:Air Pressure; D000704:Analysis of Variance; D000818:Animals; D003487:Cyanoacrylates / Q000627:therapeutic use*; D003720:Densitometry; D004659:Enbucrilate; D005187:Fallopian Tubes / Q000033:anatomy & histology / Q000601:surgery; D005260:Female; D015907:Hysteroscopy; D023421:Models, Animal; D011247:Pregnancy; D018873:Pregnancy Rate; D011817:Rabbits; D011897:Random Allocation; D013246:Sterilization, Tubal / Q000009:adverse effects / Q000379:methods*; D014014:Tissue Adhesives / Q000627:therapeutic use*
D003487:Cyanoacrylates; D014014:Tissue Adhesives; D004659:Enbucrilate