[Determination of tanshinone II A and deoxyschizandrin in danshenwuweizi tablet by RP-HPLC].
OBJECTIVE
to set up a determination of tanshinone II A and deoxyschizaadrin in danshenwuweizi tablet by RP-HPLC.


METHODS
two components were separated through Kromasil C18 (250 mm x 4.6 mm, 5 microm) column with methanol-water (85:15) by volume as a mobile phase. The flow rate was 1 ml x min(-1) and the detection wavelength were 270 nm (tanshinone II A) and 254 nm (deoxyschizandrin).


RESULTS
The linear range of tanshinone II A and deoxyschizandrin were 0.3232 microg-1.616 microg and 0.525 microg-2.625 microg, respectively. The average recovery was 99.83% with RSD 0.36% for tanshinone II A and 100.2% with RSD 0.72% for deoxyschizandrin respectively.


CONCLUSIONS
The method is simple, accurate and rapid with good reproducibility. It can be used for the quality control of danshenwuweizi tablet.
Li
Rui-Zhen
RZ
The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
Liao
Hua-Wei
HW
Chen
Fei-Yuan
FY
D045784:Abietanes; D002855:Chromatography, Thin Layer / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D010616:Phenanthrenes / Q000032:analysis* / Q000302:isolation & purification / Q000592:standards; D010946:Plants, Medicinal / Q000737:chemistry*; D011083:Polycyclic Compounds / Q000032:analysis* / Q000302:isolation & purification; D011786:Quality Control; D015203:Reproducibility of Results; D013607:Tablets
D045784:Abietanes; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D010616:Phenanthrenes; D011083:Polycyclic Compounds; D013607:Tablets; C021751:tanshinone