title: Preventive effects of diallyl sulfide on 7,12-dimethylbenz[a]anthracene induced DNA alkylation damage in mouse skin.
abstract: Mutations that occur through DNA strand breaks are the prerequisites for the development of tumors, which ultimately leads to various genetic disorders including cancer. A number of naturally occurring compounds including certain dietary constituents play an important role in causation and prevention of a number of genetic diseases. Diallyl sulfide (DAS), a volatile organosulfur compound present in garlic has been shown to possess various pharmacological effects including cancer preventive properties. Now we are reporting the antimutagenic properties of DAS on 7,12- dimethylbenz[a]anthracene (DMBA), a carcinogenic polycyclic aromatic hydrocarbon, induced DNA strand breaks in mouse skin, using an alkaline unwinding assay. DAS (2.5-10 mg/kg body-weight) was applied topically, prior and post to DMBA (5 mg/kg body-weight) at the sampling time of 24, 48, 72 and 96 h. DAS application resulted in a significant (p < 0.001) protection in DMBA-induced DNA strand breaks. The pre-treatment of DAS (10 mg/kg body-weight) showed 68.35% protection and post-treatment showed 59.49% protection, at an intermittent period of 48 h, against DMBA-induced DNA strand breakage. These findings suggest that DAS can effectively check the mutations induced by environmental toxicants.
authors.affiliation: Environmental Carcinogenesis Division, Industrial Toxicology Research Centre, Lucknow, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Nigam
authors.forename: Yogeshwer
authors.initials: Y
authors.lastname: Shukla
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology*; D000478:Alkylation; D000498:Allyl Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D016588:Anticarcinogenic Agents; D002273:Carcinogens / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D051379:Mice; D016296:Mutagenesis / Q000187:drug effects; D012867:Skin / Q000737:chemistry / Q000187:drug effects*; D013440:Sulfides / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000498:Allyl Compounds; D016588:Anticarcinogenic Agents; D002273:Carcinogens; D013440:Sulfides; D015127:9,10-Dimethyl-1,2-benzanthracene; C038491:allyl sulfide