title: Effects of short-term exercise on adiponectin and adiponectin receptor levels in rats.
abstract: OBJECTIVE
  Adiponectin reportedly reduces insulin resistance. Exercise has also been shown to lessen insulin resistance, although it is not well known whether exercise increases levels of adiponectin and/or its receptors nor whether it effects are dependent on exercise intensity and/or period. We previously reported that blood adiponectin levels increased by 150% in animals that exercised at a rate of 30 m/min for 60 minutes, 2 days per week, and adiponectin receptor 1 (AdipoR1) mRNA levels in muscle increased up to 4 times in response to exercise at a rate of 25 m/min for 30 min, 5 days per week for 12 weeks.
  
  
  METHODS
  In light of this information, we examined the effects of short-term exercise on adiponectin, and adiponectin receptor levels in rats, using ELISA and real-time PCR.
  
  
  RESULTS
  Our data showed that adiponectin mRNA levels in adipose tissue increased by 280% in rats exercised at a rate of 30 m/min for 60 minutes for 2 weeks and correlated with the exercise time periods. No effects of short-term exercise on adiponectin receptor 1 mRNA in muscle were observed.
  
  
  CONCLUSIONS
  Thus, long-term exercise may be required to regulate adiponectin receptor 1 mRNA expression in muscle and adiponectin mRNA expression in adipose tissue.
authors.affiliation: Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zeng
authors.forename: Ling
authors.initials: L
authors.lastname: Fu
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takekoshi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawakami
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Isobe
mesh_terms: D052242:Adiponectin / Q000096:biosynthesis*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D004837:Epinephrine / Q000652:urine; D015870:Gene Expression; D008055:Lipids / Q000097:blood; D010805:Physical Conditioning, Animal / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D054419:Receptors, Adiponectin / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013995:Time
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D008055:Lipids; D012333:RNA, Messenger; D054419:Receptors, Adiponectin; D004837:Epinephrine