Glutathione S-transferase theta 1 expressed in granulosa cells as a biomarker for oocyte quality in age-related infertility.
OBJECTIVE
The goal of this study was to identify a reliable biomarker for age-related infertility.


METHODS
Laboratory study.


METHODS
ART laboratory.


METHODS
Patients undergoing intracytoplasmic sperm injection or IVF cycles.


METHODS
Expression of Glutathione S-transferase (GST) mRNA and protein in mural and cumulus granulosa cells obtained from infertile patients were examined by reverse transcriptase-polymerase chain reaction and immunofluorescence.


METHODS
Correlation between the expression of GST theta 1 (GSTT1) in granulosa cells and oocyte quality was a main outcome measure.


RESULTS
Expression of GSTT1 in granulosa cells from male factor patients was positively correlated with age and negatively with cumulus-oocyte complex maturity. When samples with high and low GSTT1 in granulosa cells were extracted from the other infertility factors, cumulus-oocyte complex maturity in the high GSTT1 group was significantly lower than that in the low GSTT1 group (high: 27.2% vs. low: 51.3%). The developmental capacity of oocytes in the high GSTT1 group was likely to be lower (high: 26.4% vs. low: 43.9%). Up-regulation of GSTT1 during aging may be promoted by FSH and H(2)O(2), determined by an in vitro model.


CONCLUSIONS
GSTT1 is a good indicator for age-related infertility.
Ito
Megumu
M
Department of Perinatal Medicine and Maternal Care, Division of Reproductive Medicine, National Center for Child Health and Development, Tokyo, Japan.
Muraki
Miho
M
Takahashi
Yuji
Y
Imai
Misa
M
Tsukui
Tohru
T
Yamakawa
Naomi
N
Nakagawa
Koji
K
Ohgi
Shirei
S
Horikawa
Takashi
T
Iwasaki
Wakako
W
Iida
Ayumi
A
Nishi
Yoshihiro
Y
Yanase
Toshihiko
T
Nawata
Hajime
H
Miyado
Kenji
K
Kono
Tomohiro
T
Hosoi
Yoshihiko
Y
Saito
Hidekazu
H
D000328:Adult; D000375:Aging / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism*; D006107:Granulosa Cells / Q000201:enzymology*; D006801:Humans; D007247:Infertility, Female / Q000201:enzymology* / Q000473:pathology*; D009865:Oocytes / Q000201:enzymology* / Q000473:pathology*
D015415:Biomarkers; C413545:glutathione S-transferase T1; D005982:Glutathione Transferase