Akt is involved in the inhibition of cell proliferation by EGF.
Axin is a negative regulator of the Wnt/beta-catenin pathway and is involved in the regulation of axis formation and proliferation. Involvement of Axin in the regulation of other signaling pathways is poorly understood. In this study, we investigated the involvement of Akt in growth regulation by Axin in L929 fibroblasts stimulated by EGF. Akt activity was increased by EGF treatment and Ras activation, respectively. Both the EGF- and Ras-induced Akt activations were abolished by Axin induction, as revealed by both Western blot and immunocytochemical analyses. The proliferation and Akt activation induced by EGF were decreased by Axin induction, and the effects of EGF were abolished by treatment of an Akt-specific inhibitor. Therefore, Axin inhibits EGF-induced proliferation of L929 fibroblasts by blocking Akt activation.
Jeon
Soung Hoo
SH
Department of Biotechnology, Yonsei University, Seoul 120-752, Korea.
Jeong
Woo-Jeong
WJ
Cho
Jae-Young
JY
Lee
Kee-Ho
KH
Choi
Kang-Yell
KY
D000818:Animals; D060466:Axin Protein; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D051379:Mice; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction; D018631:ras Proteins / Q000096:biosynthesis / Q000235:genetics
D060466:Axin Protein; D012097:Repressor Proteins; D004815:Epidermal Growth Factor; D051057:Proto-Oncogene Proteins c-akt; D018631:ras Proteins