Permeability profile estimation of flavonoids and other phenolic compounds by biopartitioning micellar capillary chromatography.
This paper points out the usefulness of biopartitioning micellar chromatography (BMC) using capillary columns as a high-throughput primary screening tool providing key information about the oral absorption, skin permeability, and brain-blood distribution coefficients of 15 polyphenols (6 flavones, 2 flavonols, a flavanone, 2 flavan-3-ols, 3 phenolic acids, and a phloroglucinol) in a simple and economical way. For the compounds studied, except vicenin-2, rutin, chlorogenic acid, p-hydroxycinnamic acid, and 4-hydroxybenzoic acid, maximal oral absorption (>90%) can be expected, if there are not solubility problems or metabolic processes. On the other hand, the most retained compounds in BMC, that is, 5-hydroxyflavone, flavone, and flavanone, show the highest brain-blood distribution coefficients and skin permeability coefficients.
Diniz
Andréa
A
Departamento de Ciências Farmacêuticas, CCS, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, PR 445 km 380, CEP 86051-990, Londrina, PR, Brazil. maria.j.medina@uv.es
Escuder-Gilabert
Laura
L
Lopes
Norberto P
NP
Gobbo-Neto
Leonardo
L
Villanueva-Camañas
Rosa María
RM
Sagrado
Salvador
S
Medina-Hernández
María José
MJ
D002845:Chromatography / Q000379:methods*; D005419:Flavonoids / Q000737:chemistry* / Q000493:pharmacokinetics; D008954:Models, Biological; D010539:Permeability; D010636:Phenols / Q000737:chemistry* / Q000493:pharmacokinetics; D059808:Polyphenols
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols