[Expression of Stat3, HIF-1alpha and VEGF in Wilms' tumor].
OBJECTIVE
To study the expression of signal transducer and activator of transcription 3 (Stat3), hypoxia-inducible factor-1alpha (HIF-1alpha) and vascular endothelial growth factor (VEGF) in Wilms' tumor and their roles in the development of Wilms' tumor.


METHODS
The expression of Stat3, HIF-1alpha and VEGF were detected by the immunohistochemical staining in 52 specimens from Wilms' tumor tissues, 47 from adjacent kidney tissues and 8 from normal kidney tissues. The expression intensity was analyzed by computer image processing.


RESULTS
The expression of Stat3, HIF-1 and VEGF were significantly up-regulated in Wilms' tumor tissues compared to those in adjacent tissues and normal kidney tissues (P < 0.05). Stat3 and VEGF proteins in Wilms' tumor tissues of stage III-IV and high risk histopathology were significantly higher than those of stage I-II and low risk histopathology. The higher expression of HIF-1 in Wilms' tumor tissues was shown in tumors with high risk histopathology and tumor size > or = 6 cm.


CONCLUSIONS
Increased expression of Stat3, HIF-1 and VEGF were found in Wilms' tumor tissues, and may be related to the development and angiogenesis of Wilms' tumor. Stat3 may regulate the expression of HIF-1 and VEGF, so it could be an effective target for inhibiting VEGF expression and angiogenesis of Wilms' tumor.
Niu
Zhi-Bin
ZB
Department of Pediatric Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, China. niuzb@163.com
Wang
Chang-Lin
CL
Hou
Ying
Y
Yang
Yi
Y
Gao
Hong
H
D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000032:analysis*; D007150:Immunohistochemistry; D007223:Infant; D007680:Kidney Neoplasms / Q000098:blood supply / Q000737:chemistry* / Q000473:pathology; D008297:Male; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000209:etiology; D050796:STAT3 Transcription Factor / Q000032:analysis*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis* / Q000037:antagonists & inhibitors; D009396:Wilms Tumor / Q000098:blood supply / Q000737:chemistry* / Q000473:pathology
C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D042461:Vascular Endothelial Growth Factor A