title: Transgenic cotton expressing synthesized scorpion insect toxin AaHIT gene confers enhanced resistance to cotton bollworm (Heliothis armigera) larvae.
abstract: A synthetic scorpion Hector Insect Toxin (AaHIT) gene, under the control of a CaMV35S promoter, was cloned into cotton via Agrobacterium tumefaciens-mediated transformation. Southern blot analyses indicated that integration of the transgene varied from one to more than three estimated copies per genome; seven homozygous transgenic lines with one copy of the T-DNA insert were then selected by PCR and Southern blot analysis. AaHIT expression was from 0.02 to 0.43% of total soluble protein determined by western blot. These homozygous transgenic lines killed larvae of cotton bollworm (Heliothis armigera) by 44-98%. The AaHIT gene could used therefore an alternative to Bt toxin and proteinase inhibitor genes for producing transgenic cotton crops with effective control of bollworm.
authors.affiliation: Institute of Cotton Research and Biotechnology Research Centre, Shanxi Academy of Agricultural Sciences, Yuncheng, Taiyuan, PR China.
authors.forename: Jiahe
authors.initials: J
authors.lastname: Wu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Luo
authors.forename: Zhian
authors.initials: Z
authors.lastname: Wang
authors.forename: Yingchuan
authors.initials: Y
authors.lastname: Tian
authors.forename: Aihua
authors.initials: A
authors.lastname: Liang
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003368:Gossypium / Q000235:genetics* / Q000378:metabolism; D007814:Larva; D008969:Molecular Sequence Data; D009036:Moths; D010572:Pest Control, Biological; D030821:Plants, Genetically Modified; D012604:Scorpion Venoms / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*
chemical_list: D012604:Scorpion Venoms; C065314:scorpion toxin I, Androctonus