Degradation of cyanidin 3-rutinoside in the presence of (-)-epicatechin and litchi pericarp polyphenol oxidase.
The degradation mechanism of cyanidin 3-rutinoside in the presence of (-)-epicatechin and litchi pericarp polyphenol oxidase (PPO) was investigated using several model systems. The enzymically generated (-)-epicatechin o-quinone could induce cyanidin 3-rutinoside degradation. The results obtained in this study allowed us to propose a pathway for cyanidin 3-rutinoside degradation in the presence of (-)-epicatechin and litchi pericarp PPO. First, enzymatic oxidation of (-)-epicatechin produced the corresponding o-quinone, and then cyanidin 3-rutinoside and (-)-epicatechin competed for (-)-epicatechin o-quinone, resulting in degradation of cyanidin 3-rutinoside and regeneration of (-)-epicatechin. Moreover, the results of kinetic studies indicated this competition was influenced by both (-)-epicatechin concentration and cyanidin 3-rutinoside concentration in the model system.
Liu
Liang
L
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, People's Republic of China.
Cao
Shaoqian
S
Xie
Bijun
B
Sun
Zhida
Z
Wu
Jinjun
J
D000872:Anthocyanins / Q000378:metabolism*; D002392:Catechin / Q000378:metabolism / Q000494:pharmacology*; D004156:Catechol Oxidase / Q000378:metabolism*; D005638:Fruit / Q000201:enzymology; D007700:Kinetics; D032125:Litchi / Q000201:enzymology*; D010084:Oxidation-Reduction; D011809:Quinones / Q000378:metabolism
D000872:Anthocyanins; D011809:Quinones; D002392:Catechin; D004156:Catechol Oxidase; C428983:cyanidin 3-rutinoside