Transposon Tn7 directs transposition into the genome of filamentous bacteriophage M13 using the element-encoded TnsE protein.
The bacterial transposon Tn7 has a pathway of transposition that preferentially targets conjugal plasmids. We propose that this same transposition pathway recognizes a structure or complex found during filamentous bacteriophage replication, likely by targeting negative-strand synthesis. The ability to insert into both plasmid and bacteriophage DNAs that are capable of cell-to-cell transfer would help explain the wide distribution of Tn7 relatives.
Finn
Jessica A
JA
Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.
Parks
Adam R
AR
Peters
Joseph E
JE
D017104:Bacteriophage M13 / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000502:physiology*; D004926:Escherichia coli / Q000502:physiology / Q000821:virology; D029968:Escherichia coli Proteins / Q000502:physiology*; D016679:Genome, Viral; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic
D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C424904:TnsE protein, E coli