title: Protection against hydrogen peroxide induced oxidative damage in rat erythrocytes by Mangifera indica L. peel extract.
abstract: Phytochemicals such as polyphenols and carotenoids are gaining importance because of their contribution to human health and their multiple biological effects such as antioxidant, antimutagenic, anticarcinogenic and cytoprotective activities and other therapeutic properties. Mango peel is a major by-product in pulp industry and it contains various bioactive compounds like polyphenols, carotenoids and others. In the present study, the protective effect of peel extracts of unripe and ripe mango fruits of two varieties namely, Raspuri and Badami on hydrogen peroxide induced hemolysis, lipid peroxidation, degradation of membrane proteins and its morphological changes are reported. The oxidative hemolysis of rat erythrocytes by hydrogen peroxide was inhibited by mango peel extract in a dose dependent manner. The IC(50) value for lipid peroxidation inhibition on erythrocyte ghost membrane was found to be in the range of 4.5-19.3 microg gallic acid equivalents. The mango peel extract showed protection against membrane protein degradation caused by hydrogen peroxide. Morphological changes to erythrocyte membrane caused by hydrogen peroxide were protected by mango peel extract. The results demonstrated that mango peel extracts protected erythrocytes against oxidative stress and may impart health benefits and it could be used as a valuable food ingredient or a nutraceutical product.
authors.affiliation: Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore 570 020, India.
authors.forename: C M
authors.initials: CM
authors.lastname: Ajila
authors.forename: U J S
authors.initials: UJ
authors.lastname: Prasada Rao
mesh_terms: D000096:Acetone; D000818:Animals; D000872:Anthocyanins / Q000032:analysis; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002338:Carotenoids / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects*; D005638:Fruit / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D066298:In Vitro Techniques; D008297:Male; D031022:Mangifera / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012997:Solvents
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D012997:Solvents; D000096:Acetone; D002338:Carotenoids; D006861:Hydrogen Peroxide