Engineered synthetic pathway for isopropanol production in Escherichia coli.
A synthetic pathway was engineered in Escherichia coli to produce isopropanol by expressing various combinations of genes from Clostridium acetobutylicum ATCC 824, E. coli K-12 MG1655, Clostridium beijerinckii NRRL B593, and Thermoanaerobacter brockii HTD4. The strain with the combination of C. acetobutylicum thl (acetyl-coenzyme A [CoA] acetyltransferase), E. coli atoAD (acetoacetyl-CoA transferase), C. acetobutylicum adc (acetoacetate decarboxylase), and C. beijerinckii adh (secondary alcohol dehydrogenase) achieved the highest titer. This strain produced 81.6 mM isopropanol in shake flasks with a yield of 43.5% (mol/mol) in the production phase. To our knowledge, this work is the first to produce isopropanol in E. coli, and the titer exceeded that from the native producers.
Hanai
T
T
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531 Boelter Hall, Los Angeles, CA 90095, USA.
Atsumi
S
S
Liao
J C
JC
D019840:2-Propanol / Q000378:metabolism*; D000101:Acetyl-CoA C-Acetyltransferase / Q000235:genetics; D000429:Alcohol Oxidoreductases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D053898:Biosynthetic Pathways; D002262:Carboxy-Lyases / Q000235:genetics; D003001:Cloning, Molecular; D046969:Clostridium acetobutylicum / Q000201:enzymology / Q000235:genetics; D046970:Clostridium beijerinckii / Q000201:enzymology / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D046968:Thermoanaerobacter / Q000201:enzymology / Q000235:genetics
D001426:Bacterial Proteins; D000429:Alcohol Oxidoreductases; C025192:isopropanol dehydrogenase (NADP); D000101:Acetyl-CoA C-Acetyltransferase; D002262:Carboxy-Lyases; C023743:acetoacetate decarboxylase; D019840:2-Propanol