title: ATP plays a role in neurite stimulation with activated mast cells.
abstract: Previously, we showed that nerve-mast cell cross-talk can occur bidirectionally and that substance P is a mediator to activate mast cells. Here, we have studied the mediators to activate nerves cocultured with mast cells. Addition of antigen to the cocultures of superior cervical ganglia (SCG) and rat basophilic leukemia cells (RBLs) elicited Ca(2+) response in RBLs and after a lag period induced Ca(2+) signal in SCG neurites. Pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (purinergic receptor antagonist) or apyrase (ATP-hydrolyzing enzyme) reduced the Ca(2+) signals in neurites, indicating that ATP released from activated mast cells was one of important mediators to activate nerves.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Suzuki
authors.forename: Tadahide
authors.initials: T
authors.lastname: Furuno
authors.forename: Keisuke
authors.initials: K
authors.lastname: Okamoto
authors.forename: Reiko
authors.initials: R
authors.lastname: Teshima
authors.forename: Mamoru
authors.initials: M
authors.lastname: Nakanishi
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003469:Culture; D004140:Dinitrophenols / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006632:Histamine / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008808:Mice, Inbred CBA; D016501:Neurites / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D051381:Rats; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors; D012701:Serotonin / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D017783:Superior Cervical Ganglion / Q000166:cytology; D013997:Time Factors
chemical_list: D004140:Dinitrophenols; D011956:Receptors, Cell Surface; C034638:dinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine; D012701:Serotonin; D006632:Histamine; D000255:Adenosine Triphosphate; D002118:Calcium