title: [Enlargement test studies of bio-hydrogen production using artificial wastewater of corn stalk fermentation lixivium by mixed culture].
abstract: Conversion of artificial corn stalk wastewater, which was prepared according to the main composition of the corn stalk fermentation lixivium, into bio-hydrogen gas by mixed culture was performed in a 20 L half-continuous flow fermenter. The influences of several environmental factors on the bio-hydrogen production, such as HRT, C/N ratio, Fe2+ and artificial corn stalk wastewater concentration were discussed in the tests. The experimental results showed that HRT, C/N ratio, Fe2+ and artificial corn stalk wastewater concentration significantly affected the fermentation hydrogen production. The maximum H2 yield of 11.80 mol/kg, H2 concentration of 56% and hydrogen production rate of 8.81 L/(L x d) were obtained at HRT = 10 h, C/N = 100, Fe2+ concentration of 100 mg/L and substrate concentration of 12.5 g/L by mixed culture, respectively. In the fermentation hydrogen-producing process, the conversion efficiency of the substrate was more than 90%, and 39.40% of COD was removed from the reactor. The main by-products in the liquid phase were acetic acid, butyric acid, propionic acid and a little ethanol and butanol throughout this study.
authors.affiliation: Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China. mlzhang@zzu.edu.cn
authors.forename: Mao-Lin
authors.initials: ML
authors.lastname: Zhang
authors.forename: Rui-Xia
authors.initials: RX
authors.lastname: Wei
authors.forename: Yao-Ting
authors.initials: YT
authors.lastname: Fan
authors.forename: Yan
authors.initials: Y
authors.lastname: Xing
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Hou
mesh_terms: D001674:Bioelectric Energy Sources; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000737:chemistry; D005285:Fermentation; D005296:Ferrous Compounds / Q000737:chemistry; D006859:Hydrogen / Q000032:analysis*; D009584:Nitrogen / Q000737:chemistry; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid / Q000379:methods*; D003313:Zea mays / Q000378:metabolism*
chemical_list: D005296:Ferrous Compounds; D012722:Sewage; D002244:Carbon; D006859:Hydrogen; D009584:Nitrogen