title: Microelectrospray interface with coaxial sheath flow for high-resolution capillary electrophoresis/mass spectrometry separation.
abstract: We have fabricated a coaxial sheath liquid flow microelectrospray ionization (microESI) interface for capillary electrophoresis coupled with mass spectrometry (CE/MS). The ESI interface, which features a reduced probe diameter (130 microm i.d. x 174 microm o.d.) with a nebulizer-free format, can relatively easily electrospray a large amount of make-up sheath liquid (5-10 microL/min) over the long term (more than 80 runs) with a high degree of stability. The interface also provides higher separation qualities and improved detection sensitivities compared with a conventional ion spray (IS) interface.
authors.affiliation: Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ishihama
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Soga
mesh_terms: D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D009711:Nucleotides / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods
chemical_list: D009711:Nucleotides