Ischemia-responsive protein (irp94) gene expression in neurons.
An increased expression of the ischemia-responsive protein gene (irp94) was detected in a Mongolian gerbil brain after an ischemic injury, particularly in the cerebral cortex and hippocampus. In a rat phaeochromocytoma tumour cell line (PC12 cells), actinomycin D blocked the irp94 gene expression but cycloheximide did not. This indicates that irp94 gene expression is transcriptionally controlled. The half-life of irp94 mRNA was estimated to be approx. 5 h using 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB). In addition, irp94 expression was enhanced by either endoplasmic reticulum (ER)-stress-inducible drugs or protease inhibitors. This suggests that irp94 gene expression is strongly associated with the unfolded protein response (UPR) in neurons.
Kim
Seung-Whan
SW
Department of Emergency Medicine, Chungnam National University Hospital, Taejon 301-721, Korea.
Chung
Sung-Pil
SP
Kim
Seung-Ho
SH
Choi
Jong-Soon
JS
Kwon
Kisang
K
Kwon
O-Yu
OY
D000818:Animals; D017931:DNA Primers; D005786:Gene Expression Regulation; D005849:Gerbillinae; D050957:HSP110 Heat-Shock Proteins / Q000235:genetics*; D007511:Ischemia / Q000235:genetics; D008297:Male; D009474:Neurons / Q000502:physiology*; D016716:PC12 Cells; D010673:Pheochromocytoma; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D050957:HSP110 Heat-Shock Proteins; C494655:Hspa4 protein, rat; D012333:RNA, Messenger