Inhibition of Poly(ADP-ribose)polymerase impairs Epstein Barr Virus lytic cycle progression.
BACKGROUND
Poly(ADP-ribosylation) is a post-translational modification of nuclear proteins involved in several cellular events as well as in processes that characterize the infective cycle of some viruses. In the present study, we investigated the role of poly(ADP-ribosylation) on Epstein-Barr Virus (EBV) lytic cycle activation.


RESULTS
Inhibition of PARP-1 by 3-aminobenzamide (3-ABA) during EBV induction, diminished cell damage and apoptosis in the non-productive Raji cell line while markedly reducing the release of viral particles in the productive Jijoye cells. Furthermore, incubation with 3-ABA up-regulated the levels of LMP1 and EBNA2 latent viral proteins. At the same time, it slightly affected the expression of the immediate early BZLF1 gene, but largely down-regulated the levels of the early BFRF1 protein. The modulation of the expression of both latent and lytic EBV genes appeared to be post-transcriptionally regulated.


CONCLUSIONS
Taken together the data indicate that PARP-1 plays a role in the progression of EBV lytic cycle and therefore, PARP inhibitors might represent suitable pharmacological adjuncts to control viral spread in EBV productive infection.
Mattiussi
Stefania
S
Department of Biochemical Sciences, University "Sapienza", P,le Aldo Moro, 5, 00185, Rome, Italy. s.mattiussi@idi.it
Tempera
Italo
I
Matusali
Giulia
G
Mearini
Giulia
G
Lenti
Luisa
L
Fratarcangeli
Silvia
S
Mosca
Luciana
L
D'Erme
Maria
M
Mattia
Elena
E