title: Deletion of the LIME adaptor protein minimally affects T and B cell development and function.
abstract: LIME (Lck-interacting membrane protein) is a transmembrane adaptor that associates with the Lck and Fyn protein tyrosine kinases and with the C-terminal Src kinase (Csk). To delineate the role of LIME in vivo, LIME-deficient mice were generated. Although Lime transcripts were expressed in immature and mature B and T cells, the absence of LIME impeded neither the development nor the function of B and T cells. TCR transgenic mice deprived of LIME showed, however, a 1.8-fold enhancement in positive selection. Since B cells and activated T cells express LIME and the related adaptor NTAL, mice lacking both adaptors were generated. Double-deficient mice showed no defect in the development and function of B and T cells, and the lack of LIME had no effect on the autoimmune syndrome that develops in aged NTAL-deficient mice. In contrast to a previous report, we further showed that this autoimmune syndrome develops in the absence of T cells. Therefore, our in vivo results refute all the previous roles postulated for LIME on the basis of studies of transformed B and T cells and demonstrate that LIME has no seminal role in the signaling cassette operated by antigen receptors and coreceptors.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Marseille, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Grégoire
authors.forename: Sarka
authors.initials: S
authors.lastname: Simova
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Amandine
authors.initials: A
authors.lastname: Sansoni
authors.forename: Sylvie
authors.initials: S
authors.lastname: Richelme
authors.forename: Anja
authors.initials: A
authors.lastname: Schmidt-Giese
authors.forename: Luca
authors.initials: L
authors.lastname: Simeoni
authors.forename: Pavla
authors.initials: P
authors.lastname: Angelisova
authors.forename: Dirk
authors.initials: D
authors.lastname: Reinhold
authors.forename: Burkhart
authors.initials: B
authors.lastname: Schraven
authors.forename: Vaclav
authors.initials: V
authors.lastname: Horejsi
authors.forename: Bernard
authors.initials: B
authors.lastname: Malissen
authors.forename: Marie
authors.initials: M
authors.lastname: Malissen
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000172:deficiency / Q000276:immunology*; D000818:Animals; D015551:Autoimmunity; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D015153:Blotting, Western; D002454:Cell Differentiation / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; C480489:Lck-interacting protein, mouse