title: Protection from infection with severe acute respiratory syndrome coronavirus in a Chinese hamster model by equine neutralizing F(ab')2.
abstract: To warrant potential clinical testing, the equine anti-severe acute respiratory syndrome coronavirus (SARS-CoV) F(ab')(2) requires evaluation in as many animal models as possible. In this study, we established a new animal model, the Chinese hamster, susceptible to SARS-CoV infection. SARS-CoV could propagate effectively and sustain high levels for 1 wk in animal lungs. All animals were protected from SARS-CoV infection in preventive settings. Further, when used therapeutically this antibody led to an approximately 4-log(10) decrease in viral burden in infected animal lungs. The pathological changes in lungs correlated closely with the dose of antibody administered. The excellent preventive and therapeutic roles of equine anti-SARS-CoV F(ab')(2) in several animal models, including the novel Chinese hamster model described in this study, have provided exciting data concerning its potential clinical study.
authors.affiliation: Department of Immunology, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, People's Republic of China.
authors.forename: Deyan
authors.initials: D
authors.lastname: Luo
authors.forename: Bing
authors.initials: B
authors.lastname: Ni
authors.forename: Guangyu
authors.initials: G
authors.lastname: Zhao
authors.forename: Zhengcai
authors.initials: Z
authors.lastname: Jia
authors.forename: Lili
authors.initials: L
authors.lastname: Zhou
authors.forename: Marek
authors.initials: M
authors.lastname: Pacal
authors.forename: Liangyan
authors.initials: L
authors.lastname: Zhang
authors.forename: Songle
authors.initials: S
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Xing
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Lin
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Jintao
authors.initials: J
authors.lastname: Li
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Liang
authors.forename: Xinfu
authors.initials: X
authors.lastname: Shi
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhao
authors.forename: Liyun
authors.initials: L
authors.lastname: Zou
authors.forename: Yuzhang
authors.initials: Y
authors.lastname: Wu
authors.forename: Xiliang
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000627:therapeutic use*; D006224:Cricetinae; D003412:Cricetulus; D004195:Disease Models, Animal; D005260:Female; D006736:Horses / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000627:therapeutic use*; D008168:Lung / Q000473:pathology / Q000821:virology; D009500:Neutralization Tests; D045473:Severe acute respiratory syndrome-related coronavirus / Q000302:isolation & purification; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology / Q000517:prevention & control*
chemical_list: D000914:Antibodies, Viral; D007140:Immunoglobulin Fab Fragments