[Microbioluminescent study of the general toxicity and mutagenicity of pollutants].
Bacterial bioluminescence was applied to detection of general toxicity (MIT test) and genotoxicity (SOS-lux test) of some chemicals, seawater, and fresh water. The SOS-induced luminescence of E. coli WP2s (cda::luxCDABE) cells was higher than in E. coli C 600 (cda::luxCDABE) at 37 degrees C and pH 6.5. The mutagenic effect of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), mitomycin C, and hydrogen peroxide determined from the induction of E. coli WP2s cell luminescence was detected at lower concentrations than in the assessment of reversion frequencies. General toxicity was demonstrated by using luminescence inhibition for hydrogen peroxide, Zn2+, and Cd2+ at low concentrations. Regions of the Krasnodar Krai where sea and fresh waters exerted toxic action on luminescence were determined by the microbioluminescent method.
Maslennikova
I L
IL
Goliasnaia
N V
NV
D002104:Cadmium / Q000633:toxicity; D002413:Cations, Divalent / Q000633:toxicity; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D005618:Fresh Water; D015386:Hazardous Substances / Q000633:toxicity*; D006861:Hydrogen Peroxide / Q000633:toxicity; D049449:Luminescence; D008769:Methylnitronitrosoguanidine / Q000633:toxicity*; D016685:Mitomycin / Q000633:toxicity; D009152:Mutagenicity Tests; D013014:SOS Response, Genetics; D012623:Seawater; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015032:Zinc / Q000633:toxicity
D002413:Cations, Divalent; D015386:Hazardous Substances; D014874:Water Pollutants, Chemical; D002104:Cadmium; D008769:Methylnitronitrosoguanidine; D016685:Mitomycin; D006861:Hydrogen Peroxide; D015032:Zinc