A multi-fluorescent MDA435 cell line for mitosis inhibitor studies: simultaneous visualization of chromatin, microtubules, and nuclear envelope in living cells.
As a part of our efforts to create a pre-made multi-fluorescent cell library for various cytological assays, we made a triple-fluorescent cell line in which microtubules, chromosomes, and nuclear envelopes were visualized for simultaneous observation of spindle structure and chromosome distribution in living cells. Pilot experiments with microtubule-disturbing drugs showed the advantages of this cell line in mitosis inhibitor studies.
Fukada
Takashi
T
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Japan.
Senda-Murata
Kaori
K
Nishida
Kumi
K
Sakaushi
Shinji
S
Minamikawa
Harumi
H
Dotsu
Masaya
M
Oka
Shigenori
S
Sugimoto
Kenji
K
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D002843:Chromatin; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D008870:Microtubules; D008938:Mitosis / Q000187:drug effects*; D009685:Nuclear Envelope; D017239:Paclitaxel / Q000494:pharmacology; D010957:Plasmids
D000972:Antineoplastic Agents, Phytogenic; D002843:Chromatin; D005456:Fluorescent Dyes; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D017239:Paclitaxel