Expression patterns of the retinal development-related genes in the fetal and adult retina.
BACKGROUND
Retina is important in converting light into neural signals, but little is known about the regulatory genes essential for the retinal morphological formation, development and functional differentiation. This study aimed to investigate the mRNA expression patterns and cellular or subcellular distribution of 33 differentially expressed genes in the retina belonging to the early and middle-late embryogenesis stages as well as the early adult stage during human development.


METHODS
In situ hybridization and real-time fluorescent quantitative reverse transcription polymerase chain reaction (FQ-RT-PCR) were used to assay 33 differentially expressed genes which were screened out using microarray analysis and were not present in the retinal cDNA or the Expressed Sequence Tags (EST) database of the National Eye Institute (NEI) Genebank.


RESULTS
Nine of the 33 genes belonged to EST or the unknown cDNA fragments, and the remaining belonged to the novel genes in the retina. During the human retinal development 17 genes were down-regulated, 6 were up-regulated and the remaining 10 were relatively unchanged. Most of the genes expressed in all layers of the retina at the gestation stage, and in the fully developed retina some genes examined did show higher expression level in certain specific cells and structures such as retinal ganglion cells or the outer segment of photoreceptor cells.


CONCLUSIONS
The gene expression profile during retinal development possesses temporal and spatial distribution features, which can provide experimental evidence for further research of the functions of those genes.
Li
Hui-ming
HM
Central Laboratory, First People's Hospital, Shanghai Jiao Tong University, Shanghai 200080, China.
Wang
Feng
F
Qiu
Wei
W
Liu
Yan
Y
Huang
Qian
Q
D020224:Expressed Sequence Tags; D005333:Fetus / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D017403:In Situ Hybridization; D012333:RNA, Messenger / Q000032:analysis; D012160:Retina / Q000196:embryology* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger