title: [Development of multifunctional envelope type artificial viral-like gene delivery system].
abstract: This review introduces a new concept "Programmed Packaging" to develop a non-viral gene delivery system. Based on this concept, multifunctional envelope type nano devices (MEND) were developed for in vitro, in situ and in vivo conditions. A quantitative study to identify a rate limiting step in intracellular trafficking was also shown between viral and non-viral vectors, which indicated an important role of controlled intranuclear disposition for development a safe and efficient non-viral gene delivery system. This review will provide a future direction of non-viral gene delivery system.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12 Nishi 6, Kita-ku, Sapporo 060-0812, Japan. harasima@pharm.hokudai.ac.jp
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Harashima
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kogure
authors.forename: Yuma
authors.initials: Y
authors.lastname: Yamada
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Akita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kamiya
mesh_terms: D000818:Animals; D017209:Apoptosis; D002467:Cell Nucleus / Q000378:metabolism; D016503:Drug Delivery Systems; D004705:Endocytosis; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D008081:Liposomes; D008928:Mitochondria / Q000378:metabolism; D049329:Nanostructures; D009369:Neoplasms / Q000648:ultrastructure
chemical_list: D008081:Liposomes