Influence of platelet-rich plasma on bone regeneration: a histomorphometric study in rabbit calvaria.
OBJECTIVE
The aim of this study was to evaluate whether local application of platelet-rich plasma (PRP) would induce bone regeneration in cranial defects on rabbits.


METHODS
Twelve female New Zealand rabbits were used for this study. Two identical 10-mm-diameter bicortical cranial defects were created in each animal. One of the defects was grafted with PRP, while the contralateral was left unfilled as a negative control. Animals were sacrificed at 2, 4, 6, and 8 weeks after surgery, and biopsy specimens were evaluated histologically and histomorphometrically under light microscopy. Analysis of variance was used for statistical analysis.


RESULTS
The histomorphometric evaluation showed more regenerated bone after local administration of PRP at 2 weeks (P > .05), 4 weeks (P < .05), and 6 weeks (P > .05). At week 8, new bone formation was comparable in both groups.


CONCLUSIONS
In this animal model, local application of PRP in bone defects enhances healing significantly at 4 weeks.
Torres
Jesús
J
Department of Health Sciences III, Faculty of Health Sciences, Rey Juan Carlos University, Alcorcón, Madrid, Spain. chustg@yahoo.es
Tresguerres
Isabel
I
Tamimi
Faleh
F
Clemente
Celia
C
Niembro
Enrique
E
Blanco
Luis
L
D000818:Animals; D001847:Bone Diseases / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D001861:Bone Regeneration / Q000502:physiology*; D005260:Female; D007091:Image Processing, Computer-Assisted; D010012:Osteogenesis / Q000502:physiology; D053657:Platelet-Rich Plasma; D011817:Rabbits; D012886:Skull / Q000473:pathology* / Q000503:physiopathology; D013997:Time Factors