Microarray-based genomic selection for high-throughput resequencing.
We developed a general method, microarray-based genomic selection (MGS), capable of selecting and enriching targeted sequences from complex eukaryotic genomes without the repeat blocking steps necessary for bacterial artificial chromosome (BAC)-based genomic selection. We demonstrate that large human genomic regions, on the order of hundreds of kilobases, can be enriched and resequenced with resequencing arrays. MGS, when combined with a next-generation resequencing technology, can enable large-scale resequencing in single-investigator laboratories.
Okou
David T
DT
Department of Human Genetics, Emory University School of Medicine, 615 Michael Street, Suite 301, Atlanta, Georgia 30322, USA.
Steinberg
Karyn Meltz
KM
Middle
Christina
C
Cutler
David J
DJ
Albert
Thomas J
TJ
Zwick
Michael E
ME
D000818:Animals; D005057:Eukaryotic Cells / Q000378:metabolism; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000235:genetics; D015723:Gene Library; D016678:Genome / Q000235:genetics*; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods*; D015534:Trans-Activators / Q000235:genetics
D051860:Fragile X Messenger Ribonucleoprotein 1; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D015534:Trans-Activators; C099930:AFF2 protein, human; C497683:FMR1 protein, human