title: MR imaging detection of superparamagnetic iron oxide loaded tris-acryl embolization microspheres.
abstract: OBJECTIVE
  To assess by magnetic resonance (MR) imaging the detectability of superparamagnetic iron oxide (SPIO)-labeled microspheres (MSs) in vitro on gelose, ex vivo in kidneys from embolized sheep, and in vivo in kidneys from embolized pigs.
  
  
  METHODS
  With various sizes of SPIO-labeled MSs, common neck and pelvic spin-echo and gradient-echo sequences were acquired on a 1.5-T MR unit. SPIO-labeled MSs of four sizes were embedded in a hydrogel as single MSs or in multiple units, or multiplets. Detection rate on MR imaging was assessed according to the real size and number of MSs. SPIO-loaded and unloaded MSs of four sizes were injected into eight sheep kidneys, which underwent MR and pathologic examinations. For each size, the location of MSs in renal vasculature was determined and compared according to the technique used. Kidneys were embolized in pigs with various amounts of MSs in three sizes. MR was performed immediately after embolization and SPIO-labeled MS detection was assessed according to size, organ, and amount injected. Results SPIO-labeled MSs provide a low signal intensity on T1-weighted sequences, without distortion. In vitro, 28% of 100-300-microm single MSs were detected and more than 80% were detected for larger sizes. MS multiplets were all detected in all sizes. Ex vivo, all sizes of MSs were detected by MR imaging in kidneys, whereas control MSs were not observed. Histologic analysis showed that there was no difference in vascular distribution between SPIO-labeled MS and control MSs, and therefore for each caliber (P > .05). Arterial location of SPIO-labeled MSs was the same on MR imaging and histologic analysis. In vivo, SPIO-labeled MS were detected in the kidney vasculature when volumes greater than 1 mL of 100-300-microm or 500-700-microm MSs were injected. Volumes lower than 1 mL SPIO-labeled MSs were hardly detected in kidneys, regardless of MS size. Conclusions SPIO-labeled MSs are detected by MR imaging with common gradient-echo sequences in vitro in gelose and ex vivo and in vivo in kidneys. SPIO-labeled MSs could allow better control of embolization and thereby enhance efficacy and safety of the procedure.
authors.affiliation: Department of Interventional Neuroradiology, Lariboisière AP-HP Hospital, Paris, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Namur
authors.forename: René
authors.initials: R
authors.lastname: Chapot
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Pelage
authors.forename: Michel
authors.initials: M
authors.lastname: Wassef
authors.forename: François
authors.initials: F
authors.lastname: Langevin
authors.forename: Denis
authors.initials: D
authors.lastname: Labarre
authors.forename: Alex
authors.initials: A
authors.lastname: Laurent
mesh_terms: D000180:Acrylic Resins / Q000008:administration & dosage*; D000818:Animals; D004621:Embolization, Therapeutic / Q000379:methods*; D005780:Gelatin / Q000008:administration & dosage*; D007261:Infusions, Intra-Arterial; D007501:Iron / Q000008:administration & dosage*; D007668:Kidney / Q000098:blood supply / Q000473:pathology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008863:Microspheres; D010087:Oxides / Q000008:administration & dosage*; D010316:Particle Size; D012077:Renal Artery; D012079:Renal Circulation; D012756:Sheep; D013552:Swine
chemical_list: D000180:Acrylic Resins; D010087:Oxides; C094094:carboxydextran-coated magnetic iron oxide particles; C496735:trisacryl gelatin microspheres; D005780:Gelatin; D007501:Iron