title: Expression of glycerol 3-phosphate dehydrogenase gene (CvGPD1) in salt-tolerant yeast Candida versatilis is stimulated by high concentrations of NaCl.
abstract: We cloned the glycerol 3-phosphate dehydrogenase (GPDH) gene (CvGPD1) from salt-tolerant yeast Candida versatilis. When CvGPD1 was expressed in glycerol synthesis-deficient Saccharomyces cerevisiae cells, the salt tolerance of the recombinant strain was enhanced, and NADP(+)-dependent GPDH (EC 1.1.1.94), Cvgpd1p synthesis and recovery of glycerol synthesis were confirmed. The transcription of CvGPD1 in C. versatilis cells was stimulated by high concentrations of NaCl. The relationship between expression of CvGPD1 and growth of C. versatilis cells in the mash of Japanese seasonings (miso- and shoyu-moromi) is also discussed.
authors.affiliation: Department of Biological Resources, National University Corporation Ehime University, Matsuyama, Ehime, Japan. watanabe@agr.ehime-u.ac.jp
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Kisako
authors.initials: K
authors.lastname: Nagayama
authors.forename: Youichi
authors.initials: Y
authors.lastname: Tamai
mesh_terms: D000595:Amino Acid Sequence; D002175:Candida / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+) / Q000235:genetics*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D012965:Sodium Chloride; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+)