[The evaluation of the techniques used for diagnosis of Entamoeba histolytica in stool specimens].
In this study, stool samples of 9378 patients from different clinics, who presented at the laboratory of the department of parasitology of the Dokuz Eylul University, Faculty of Medicine with several gastrointestinal complaints from January 2004 to May 2006, were examined. All stool samples were examined with the saline-Lugol method and, in suspicious cases, by trichrome staining, cultivation in Robinson's medium and/or antigen detection in stool with the Entamoeba CELISA Path kit. Forty-one cases (0.44%), in which Entamoeba histolytica/Entamoeba dispar cysts and/or trophozoites were detected by at least one method, were found to be positive. Out of these 41 cases, four methods were used in 24 cases, three methods in 14 cases, whereas only saline-Lugol and trichrome staining methods were used in 3 cases. Even though all 41 positive cases had been examined with the saline-Lugol method, only 25 cases were found to be positive with this method for E. histolytica/E. dispar cysts and/or trophozoites. The remaining 16 cases were diagnosed by the other three methods. Today it is necessary to distinguish E. histolytica from E. dispar because the patient does not need to be treated if E. dispar is identified whereas if E. histolytica is identified the patient needs urgent treatment. That's why it is necessary to get reliable results using diagnostic methods together and, when needed, by ELISA specific for E. histolytica.
Tuncay
Sema
S
Dokuz Eylül Universitesi Tip Fakültesi, Parazitoloji Anabilim Dali, Izmir, Turkey.
Inceboz
Tonay
T
Over
Leyla
L
Yalçin
Gülter
G
Usluca
Selma
S
Sahin
Serap
S
Delibaş
Songül Bayram
SB
Aksoy
Umit
U
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D001391:Azo Compounds; D003470:Culture Media; D003937:Diagnosis, Differential; D004404:Dysentery, Amebic / Q000175:diagnosis* / Q000469:parasitology; D004748:Entamoeba histolytica / Q000276:immunology / Q000302:isolation & purification*; D004749:Entamoebiasis / Q000175:diagnosis* / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay; D004801:Eosine Yellowish-(YS); D005243:Feces / Q000469:parasitology*; D006801:Humans; D007454:Iodides; D008739:Methyl Green; D015203:Reproducibility of Results; D012965:Sodium Chloride; D013194:Staining and Labeling / Q000379:methods
D000953:Antigens, Protozoan; D001391:Azo Compounds; D003470:Culture Media; D007454:Iodides; C017768:trichrome stain; D012965:Sodium Chloride; D008739:Methyl Green; C010389:Lugol's solution; D004801:Eosine Yellowish-(YS)