Copolymers including L-histidine and hydrophobic moiety for preparation of nonbiofouling surface.
A new type of copolymer composed of l-histidine (ampholyte) and n-butyl methacrylate (hydrophobic moiety) was developed for the preparation of nonbiofouling surfaces. The copolymer adsorbed onto resin surfaces and made the surface very hydrophilic. The hydrophilization effect was higher than that of bovine serum albumin (BSA). When polystyrene surfaces were coated with the copolymer, both the nonspecific adsorption of protein and the adhesion of cells were significantly reduced in comparison with BSA coating. The newly synthesized polymer is a new and useful candidate for the preparation of nonbiofouling surfaces.
Ishii
Takehiko
T
Nano Medical Engineering Laboratory, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama, Japan.
Wada
Akira
A
Tsuzuki
Saki
S
Casolaro
Mario
M
Ito
Yoshihiro
Y
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D006639:Histidine / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D008970:Molecular Weight; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties
D011108:Polymers; D012710:Serum Albumin, Bovine; D006639:Histidine; D006735:Horseradish Peroxidase