Chronic administration of lipopolysaccharide and proteases induces periodontal inflammation and hepatic steatosis in rats.
BACKGROUND
Epidemiologic studies suggest a relationship between periodontitis and liver diseases. A rat periodontitis model was used to investigate whether a causal relationship exists between periodontitis and liver diseases.


METHODS
Fourteen male Wistar rats (8 weeks old) were divided into two groups: a periodontitis group in which Escherichia coli lipopolysaccharide (LPS) and Streptomyces griseus proteases were applied into the gingival sulcus for 8 weeks, and a control group using pyrogen-free water instead. After blood samples were collected, periodontal tissues and liver specimens were analyzed.


RESULTS
Chronic administration of LPS and proteases to the gingival sulcus induced periodontitis and liver injury, including steatosis with inflammation and sinusoidal fibrosis. Apoptosis, enhanced concentration of 8-hydroxydeoxyguanosine, and activated production of tumor necrosis factor-alpha in liver were observed in the periodontitis group, with increased gingival inflammation, serum LPS, and reactive oxygen species.


CONCLUSIONS
Periodontal inflammation in a rat model induced fatty liver disease through increased serum LPS.
Tomofuji
Takaaki
T
Department of Oral Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Ekuni
Daisuke
D
Yamanaka
Reiko
R
Kusano
Hiroki
H
Azuma
Tetsuji
T
Sanbe
Toshihiro
T
Tamaki
Naofumi
N
Yamamoto
Tatsuo
T
Watanabe
Tatsuo
T
Miyauchi
Mutsumi
M
Takata
Takashi
T
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D053938:DNA Fragmentation; D004272:DNA, Mitochondrial / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005234:Fatty Liver / Q000209:etiology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000097:blood / Q000494:pharmacology; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism; D010447:Peptide Hydrolases / Q000494:pharmacology; D010518:Periodontitis / Q000139:chemically induced / Q000150:complications*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D004272:DNA, Mitochondrial; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D000080242:8-Hydroxy-2'-Deoxyguanosine; D010447:Peptide Hydrolases; D003849:Deoxyguanosine