The radiosensitizing effect of doranidazole on human colorectal cancer cells exposed to high doses of irradiation.
BACKGROUND
This paper investigates the effects of a new radiosensitizer, doranidazole, and enhancing irradiation on colorectal cancer cells.


METHODS
The radiosensitizing effect of doranidazole was determined using colony formation and propidium iodide (PI) assays to measure cell growth inhibition and the cell killing effect of human colorectal cancer cell lines exposed to high doses of gamma-ray irradiation under hypoxic conditions in vitro. Fluorescence staining and cell migration assays were also used to assess the radiosensitizing effect.


RESULTS
Cell proliferation evaluated by clonogenic survival curves was significantly inhibited by 5 mmol/L doranidazole, particularly at doses ranging from 10 to 30 Gy of irradiation. The radiosensitizing effect of doranidazole on colorectal cancer cells occurs in a time- and dose-dependent manner. Doranidazole also inhibited the mobility of cell invasion and migration.


CONCLUSIONS
Doranidazole can enhance the killing effect and the cell growth inhibition of colorectal cancer after high-dose irradiation in a time and dose-dependent manner.
Zhang
Li
L
Department of Central Laboratory, Dalian Municipal Central Hospital, Xinan-Road 826, Dalian, 116033, China. tyouri19652004@yahoo.com.cn
Gong
Aimin
A
Ji
Jun
J
Wu
Yuanyuan
Y
Zhu
Xiaoyu
X
Lv
Suqing
S
Lv
Hongzhu
H
Sun
Xizhuo
X
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000532:radiotherapy*; D005720:Gamma Rays / Q000627:therapeutic use; D006801:Humans; D007093:Imidazoles / Q000627:therapeutic use*; D011838:Radiation-Sensitizing Agents / Q000627:therapeutic use*
D007093:Imidazoles; D011838:Radiation-Sensitizing Agents; C106738:doranidazole