title: Crystallization and preliminary crystallographic analysis of the ferredoxin component of carbazole 1,9a-dioxygenase from Nocardioides aromaticivorans IC177.
abstract: Carbazole 1,9a-dioxygenase (CARDO) catalyzes the dihydroxylation of carbazole by angular position (C9a) carbon bonding to the imino nitrogen and its adjacent C1 carbon. CARDO consists of a terminal oxygenase component and two electron-transfer components: ferredoxin and ferredoxin reductase. The ferredoxin component of carbazole 1,9a-dioxygenase from Nocardioides aromaticivorans IC177 was crystallized at 293 K using the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant. The crystals, which were improved by macroseeding, diffract to 2.0 A resolution and belong to space group P4(1)2(1)2.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Inoue
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ashikawa
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Usami
authors.forename: Haruko
authors.initials: H
authors.lastname: Noguchi
authors.forename: Zui
authors.initials: Z
authors.lastname: Fujimoto
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Yamane
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nojiri
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D049308:Dioxygenases / Q000032:analysis / Q000737:chemistry*; D005288:Ferredoxins / Q000032:analysis / Q000737:chemistry*; D009618:Nocardiaceae / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D005288:Ferredoxins; D049308:Dioxygenases; C108434:carbazole 1,9a-dioxygenase