Natural Tregs, CD4+CD25+ inhibitory hybridomas, and their cell contact dependent suppression.
The natural CD4+CD25+ T regulatory (Treg) lymphocyte has emerged as a critical cell for controlling immune responses to self, foreign proteins, and pathogens. Identified initially by the constitutive expression of CD4 and CD25, natural Tregs suppress a variety of immune cells and responses, including CD4+CD25- proliferation and IL-2 production, and CD8 cell proliferation, IFNgamma production and CTL activity. Although natural Tregs require activation with specific antigen to attain their suppressive phenotype, once activated they execute inhibition in an antigen specific as well as non-specific (bystander) fashion. Treg suppression depends on IL-2, CD25, and cell:cell contact. The use of live cell imaging in vivo and in vitro to visualize the dynamic cell:cell interactions involving natural Tregs as well as the CD4+CD25+ Treg inhibitory hybridoma RD6 has refined the mechanistic models of contact dependent Treg suppression.
Field
Elizabeth H
EH
Department of Medicine, Division of Rheumatology, Roy J and Lucille A Carver College of Medicine, Iowa City, IA, USA. liz-field@icva.gov
Kulhankova
Katarina
K
Nasr
Mohamed E
ME
D000818:Animals; D015551:Autoimmunity; D015704:CD4 Antigens / Q000032:analysis; D002450:Cell Communication; D006084:Graft Rejection; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007108:Immune Tolerance; D007376:Interleukin-2 / Q000276:immunology / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000276:immunology / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
D015704:CD4 Antigens; D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit; D011948:Receptors, Antigen, T-Cell; D015375:Receptors, Interleukin-2