[PCR-RFLP analysis of the Tams1 gene of Theileria annulata].
Tams1 is a merozoite surface antigen of Theileria annulata. Genetical variations of Tams1 make studies of vaccine and diagnostic tests like ELISA difficult. In this study, Tams1 genes of 89 T. annulata isolates obtained from natural infected cattle in Elazig and Bingöl regions were tested with PCR-RFLP. Six different restriction profiles (a, b, c, d, e, f) were detected. The number of restriction profiles of 89 samples was found to be as follows: 78(a), 2(b), 2(c), 5(d), 1(e), and 1(f).
Altay
Kürşat
K
Firat Universitesi, Veteriner Fakültesi Parazitoloji Anabilim Dali, Elaziğ, Turkey. kaltay@firat.edu.tr
Aktaş
Münir
M
Dumanli
Nazir
N
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D002417:Cattle; D016054:DNA, Protozoan / Q000737:chemistry* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012150:Polymorphism, Restriction Fragment Length; D016796:Theileria annulata / Q000235:genetics* / Q000276:immunology; D013801:Theileriasis / Q000469:parasitology; D014421:Turkey
D000953:Antigens, Protozoan; D016054:DNA, Protozoan; C113538:Tams1 antigen