Amperometric glucose biosensors based on layer-by-layer assembly of chitosan and glucose oxidase on the Prussian blue-modified gold electrode.
A glucose biosensor based on layer-by-layer (LBL) self-assembling of chitosan and glucose oxidase (GOD) on a Prussian blue film was developed. First, Prussian blue was deposited on a cleaned gold electrode then chitosan and GOD were assembled alternately to construct a multilayer film. The resulting amperometric glucose biosensor exhibited a fast response time (within 10 s) and a linear calibration range from 6 microM to 1.6 mM with a detection limit of 3.1 microM glucose (s/n = 3). With the low operating potential, the biosensor showed little interference to the possible interferents, including ascorbic acid, acetaminophen and uric acid, indicating an excellent selectivity.
Yin
Bing
B
Chongqing Key Laboratory of Analytical Chemistry, College of Chemistry and Engineering, Southwest University, Chongqing 400715, PR China.
Yuan
Ruo
R
Chai
Yaqing
Y
Chen
Shihong
S
Cao
Shurui
S
Xu
Yang
Y
Fu
Ping
P
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D048271:Chitosan / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry; D005947:Glucose / Q000032:analysis / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*
D004396:Coloring Agents; D005295:Ferrocyanides; D006046:Gold; D048271:Chitosan; D005949:Glucose Oxidase; D005947:Glucose; C000170:ferric ferrocyanide