title: Effect of nicotine and polyaromtic hydrocarbons on cerebral endothelial cells.
abstract: The present study was designed to investigate the effect of nicotine and polyaromatic hydrocarbon compounds on cerebral endothelial cells (CECs). Nicotine treatments from 15 min to 5h did not cause any changes in the expression and localization of principal junctional proteins. One day of treatment with a relatively high concentration of nicotine induced a decrease in the expression of the tight junction protein ZO-1, occludin, and the adherens junction protein, cadherin. Treatment with 3 x 10(-5)M phenanthrene for 24h caused a redistribution of occludin from the Triton X-100 insoluble to the Triton X-100 soluble fraction. Transendothelial electrical resistance was not significantly affected by 24h treatments with nicotine, methylanthracene or phenanthrene. However, 24h nicotine treatment increased transendothelial permeability in CECs exposed to oxidative stress. Both nicotine and phenanthrene were able to regulate the expression of a large number of proteins as revealed by 2D electrophoresis. Our experiments suggest that tobacco smoking may affect the junctional complex of CECs, and that this effect is enhanced by oxidative stress.
authors.affiliation: Institute of Biophysics, Biological Research Centre, Temesvári krt. 62, 6726 Szeged, Hungary.
authors.forename: Pilaiwanwadee
authors.initials: P
authors.lastname: Hutamekalin
authors.forename: Attila E
authors.initials: AE
authors.lastname: Farkas
authors.forename: Anna
authors.initials: A
authors.lastname: Orbók
authors.forename: Imola
authors.initials: I
authors.lastname: Wilhelm
authors.forename: Péter
authors.initials: P
authors.lastname: Nagyoszi
authors.forename: Szilvia
authors.initials: S
authors.lastname: Veszelka
authors.forename: Mária A
authors.initials: MA
authors.lastname: Deli
authors.forename: Krisztina
authors.initials: K
authors.lastname: Buzás
authors.forename: Eva
authors.initials: E
authors.lastname: Hunyadi-Gulyás
authors.forename: Katalin F
authors.initials: KF
authors.lastname: Medzihradszky
authors.forename: Duangdeun
authors.initials: D
authors.lastname: Meksuriyen
authors.forename: István A
authors.initials: IA
authors.lastname: Krizbai
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000502:physiology; D015820:Cadherins / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology*; D062507:Claudin-5; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000187:drug effects*; D008565:Membrane Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009285:Naphthoquinones / Q000494:pharmacology; D009457:Neuroglia / Q000166:cytology; D009538:Nicotine / Q000494:pharmacology*; D018722:Nicotinic Agonists / Q000494:pharmacology*; D062793:Occludin; D018384:Oxidative Stress; D010616:Phenanthrenes / Q000494:pharmacology; D010750:Phosphoproteins / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000494:pharmacology*; D020543:Proteome / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein; D051176:beta Catenin / Q000378:metabolism
chemical_list: D015820:Cadherins; D062507:Claudin-5; C491510:Cldn5 protein, rat; D008565:Membrane Proteins; D009285:Naphthoquinones; D018722:Nicotinic Agonists; D062793:Occludin; C568987:Ocln protein, rat; D010616:Phenanthrenes; D010750:Phosphoproteins; D011084:Polycyclic Aromatic Hydrocarbons; D020543:Proteome; C569044:Tjp1 protein, rat; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein; D051176:beta Catenin; C031181:phenanthrene; C063002:2,3-dimethoxy-1,4-naphthoquinone; D009538:Nicotine