Catechin degradation with concurrent formation of homo- and heterocatechin dimers during in vitro digestion.
Catechins were subjected to in vitro gastric and small intestinal digestion. EGCG, EGC, and ECG were significantly degraded at all concentrations tested, with losses of 71-91, 72-100, and 60-61%, respectively. EC and C were comparatively stable, with losses of 8-11 and 7-8%, respectively. HLPC-ESI-MS/MS indicated that EGCG degradation under simulated digestion resulted in production of theasinensins (THSNs) A and D (m/z 913) and P-2 (m/z 883), its autoxidation homodimers. EGC dimerization produced the homodimers THSN C and E (m/z 609) and homodimers analogous to P-2 (m/z 579). ECG homodimers were not observed. EGCG and EGC formed heterodimers analogous to the THSNs (m/z 761) and P-2 (m/z 731). EGCG and ECG formed homodimers analogous to the THSNs (m/z 897). This study provides an expanded profile of catechin dimers of digestive origin that may potentially form following consumption of catechins. These data provide a logical basis for initial screening to detect catechin digestive products in vivo.
Neilson
Andrew P
AP
Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, Indiana 47907, USA
Hopf
Amber S
AS
Cooper
Bruce R
BR
Pereira
Michael A
MA
Bomser
Joshua A
JA
Ferruzzi
Mario G
MG
D002392:Catechin / Q000031:analogs & derivatives / Q000378:metabolism*; D004063:Digestion; D019281:Dimerization; D066298:In Vitro Techniques; D008049:Lipase / Q000378:metabolism; D010084:Oxidation-Reduction; D010194:Pancreatin / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism
D010194:Pancreatin; D002392:Catechin; C062669:epicatechin gallate; C045651:epigallocatechin gallate; D008049:Lipase; D010434:Pepsin A; C057580:gallocatechol