Determination of human papillomavirus type 16 genotype and construction of cloning vector pTZ57R encoding HPV16 E7 gene.
OBJECTIVE
To isolate and construct a cloning vector containing the human papillomavirus (HPV)16-E7 gene as a target for application as a DNA vaccine.


METHODS
The study was performed in 2005 in Iran. The E7 gene, one of the most important HPV oncoproteins and a target molecule for therapeutic vaccines, was amplified by polymerase chain reaction (PCR). The PCR product was cloned into a suitable cloning vector and confirmed by colony-PCR, restriction enzyme analysis, and sequenced.


RESULTS
The desired plasmid was sequenced and indicated 99% homology with those mentioned in the Genbank.


CONCLUSIONS
The Iranian HPV16 E7 gene sequence is very similar to other sequences in the Genbank, and it can be used as a candidate gene in a therapeutic vaccine for Iranian patients with cervical cancer.
Meshkat
Zahra
Z
Department of Virology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Soleimanjahi
Hoorieh
H
Mahmoudi
Mahmoud
M
Mirshahabi
Hessam
H
Hassan
Zuhair M
ZM
Ghaffari
Saeed R
SR
Sabokbar
Tayebeh
T
D005260:Female; D018390:Gene Targeting; D005822:Genetic Vectors; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007492:Iran; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D053918:Papillomavirus Vaccines; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000517:prevention & control / Q000821:virology*
D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D053918:Papillomavirus Vaccines; C059731:oncogene protein E7, Human papillomavirus type 16