Identification of a metagenome-derived esterase with high enantioselectivity in the kinetic resolution of arylaliphatic tertiary alcohols.
35 metagenome-derived esterases bearing a GGG(A)X motif were screened for activity and enantioselectivity in the hydrolysis of a range of tertiary alcohol acetates. Most of the active esterases showed little or no enantioselectivity in the hydrolysis of the terpinyl acetate, linalyl acetate and 3-methylpent-1-yn-3-yl acetate. However, one esterase showed excellent enantioselectivity (E > 100) in the kinetic resolution of 1,1,1-trifluoro-2-phenylbut-3-yn-2-yl acetate as confirmed by a preparative scale reaction.
Kourist
Robert
R
Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany.
Hari Krishna
Sajja
S
Patel
Jesal S
JS
Bartnek
Flash
F
Hitchman
Timothy S
TS
Weiner
David P
DP
Bornscheuer
Uwe T
UT
D000085:Acetates / Q000737:chemistry*; D004950:Esterases / Q000737:chemistry* / Q000235:genetics*; D015723:Gene Library; D016678:Genome; D007700:Kinetics
D000085:Acetates; D004950:Esterases