Enzymatic synthesis of hydroxycinnamic acid glycerol esters using type A feruloyl esterase from Aspergillus niger.
We found that hydroxycinnamic acid (HA) glycerol esters such as 1-sinapoyl glycerol and 1-p-coumaroyl glycerol can be synthesized through a direct esterification reaction using a type A feruloyl esterase from Aspergillus niger. The water solubilities of HA glycerol esters were higher than those of the original chemicals. HA glycerol esters absorbed ultraviolet light and scavenged 1,1-diphenyl-2-picrylhydrazyl radicals.
Tsuchiyama
Moriyasu
M
Department of Research and Development, Okumoto Flour Milling Co., Ltd, Japan.
Sakamoto
Tatsuji
T
Tanimori
Shinji
S
Murata
Shuichi
S
Kawasaki
Haruhiko
H
D000975:Antioxidants / Q000737:chemistry; D001234:Aspergillus niger / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D003373:Coumaric Acids / Q000737:chemistry / Q000378:metabolism*; D004952:Esters / Q000737:chemistry / Q000378:metabolism; D005990:Glycerol / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D013379:Substrate Specificity; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
D000975:Antioxidants; D003373:Coumaric Acids; D004952:Esters; D014867:Water; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D005990:Glycerol