[Sequence analysis of a novel HLA allele B*5618].
OBJECTIVE
To identify HLA novel allele in Chinese Han individual.


METHODS
An unknown HLA-B allele which was similar to HLA-B*5610 was detected by polymerase chain reaction-sequence specific oligonucleotide probes(PCR-SSOP), PCR-sequence specific primer(PCR-SSP) and heterozygous sequence-based typing (SBT) in a Chinese Han individual. Its anomalous patterns suggested the possible presence of new allele. The HLA-B*56 allele was amplified separately by using allele-specific primers and sequencing exons 2-4 in both directions. The differences between the novel B*56 allele and B 5610 were identified.


RESULTS
There were 4nt changes from B*5610 in exon 3, at nt379 where C>G (codon 127 CTG>GTG, 127 Leu>Val); nt412 where A>G (codon 138 AAC>GAC, 138 Asn>Asp), nt419 where T>C and nt420 where A>C (codon 140 TTA>TCC, 140 Leu>Ser). The sequence was submitted to Genbank and the accession number was EF016753.


CONCLUSIONS
This allele is a novel HLA-B allele, and has been officially named HLA-B*5618 by the WHO Nomenclature Committee in September 2006.
Zou
Hong-yan
HY
Shenzhen Institute of Transfusion Medicine, Shenzhen Blood Center, Shenzhen, Guangdong, 518035 PR China. hyan218@163.com
Li
Zhen
Z
Shao
Chao-peng
CP
Cheng
Liang-hong
LH
Jin
Shi-zheng
SZ
Zhou
Dan
D
Xiong
Wen
W
D000483:Alleles; D002681:China / Q000208:ethnology; D005006:Ethnicity / Q000235:genetics; D005091:Exons; D005787:Gene Frequency; D015235:HLA-B Antigens / Q000235:genetics*; D006239:Haplotypes; D006579:Heterozygote; D006801:Humans; D008297:Male; D015345:Oligonucleotide Probes / Q000235:genetics; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D015235:HLA-B Antigens; C520607:HLA-B*56:18 antigen; D015345:Oligonucleotide Probes