title: A non-receptor-mediated mechanism for internalization of molecular chaperones.
abstract: The evolving realization that stress proteins, which have for many years been considered to be exclusively intracellular molecules under normal conditions, can be released from viable cells via a number of potential routes/pathways has prompted interest into their extracellular biology and intercellular signaling properties. That the stress proteins Hsp60, Hsp70 and gp96 can elicit both pro- and anti-inflammatory effects suggests that these molecules play a key role in the maintenance of immunological homeostasis, and a better understanding of the immunobiology of extracellular stress proteins might reveal new and more effective approaches for controlling and managing infectious disease, inflammatory disease and cancer. A number of cell surface receptors for stress proteins have been identified, and the intracellular consequences of these cell surface receptor-ligand interactions have been characterized. To date, studies into the intercellular signaling properties of stress proteins and their interactions with antigen presenting cells have focused on specific receptor-mediated uptake, and have not considered the fact that such cells can also take up proteins via non-specific endocytosis/pinocytosis. Herein we present a methodological approach for assessing receptor-mediated and non-receptor-mediated uptake of gp96 by rat bone marrow-derived dendritic cells.
authors.affiliation: Immunobiology Research Unit, School of Medicine and Biomedical Sciences, University of Sheffield, L Floor, Royal Hallamshire Hospital, Glossop Road, Sheffield S10 2JF, UK. g.pockley@sheffield.ac.uk
authors.forename: A Graham
authors.initials: AG
authors.lastname: Pockley
authors.forename: Barbara
authors.initials: B
authors.lastname: Fairburn
authors.forename: Shabana
authors.initials: S
authors.lastname: Mirza
authors.forename: Laura K
authors.initials: LK
authors.lastname: Slack
authors.forename: Kay
authors.initials: K
authors.lastname: Hopkinson
authors.forename: Munitta
authors.initials: M
authors.lastname: Muthana
mesh_terms: D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000502:physiology; D008562:Membrane Glycoproteins / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018832:Molecular Chaperones / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012710:Serum Albumin, Bovine / Q000502:physiology
chemical_list: D008562:Membrane Glycoproteins; D018832:Molecular Chaperones; C053387:endoplasmin; D012710:Serum Albumin, Bovine