Transgenic cotton expressing synthesized scorpion insect toxin AaHIT gene confers enhanced resistance to cotton bollworm (Heliothis armigera) larvae.
A synthetic scorpion Hector Insect Toxin (AaHIT) gene, under the control of a CaMV35S promoter, was cloned into cotton via Agrobacterium tumefaciens-mediated transformation. Southern blot analyses indicated that integration of the transgene varied from one to more than three estimated copies per genome; seven homozygous transgenic lines with one copy of the T-DNA insert were then selected by PCR and Southern blot analysis. AaHIT expression was from 0.02 to 0.43% of total soluble protein determined by western blot. These homozygous transgenic lines killed larvae of cotton bollworm (Heliothis armigera) by 44-98%. The AaHIT gene could used therefore an alternative to Bt toxin and proteinase inhibitor genes for producing transgenic cotton crops with effective control of bollworm.
Wu
Jiahe
J
Institute of Cotton Research and Biotechnology Research Centre, Shanxi Academy of Agricultural Sciences, Yuncheng, Taiyuan, PR China.
Luo
Xiaoli
X
Wang
Zhian
Z
Tian
Yingchuan
Y
Liang
Aihua
A
Sun
Yi
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003368:Gossypium / Q000235:genetics* / Q000378:metabolism; D007814:Larva; D008969:Molecular Sequence Data; D009036:Moths; D010572:Pest Control, Biological; D030821:Plants, Genetically Modified; D012604:Scorpion Venoms / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*
D012604:Scorpion Venoms; C065314:scorpion toxin I, Androctonus