title: Improving the insecticidal activity against resistant Culex quinquefasciatus mosquitoes by expression of chitinase gene chiAC in Bacillus sphaericus.
abstract: Expression of a chitinase gene, chiAC, from Bacillus thuringiensis in B. sphaericus 2297 using the binary toxin promoter yielded a recombinant strain that was 4,297-fold more toxic than strain 2297 against resistant Culex quinquefasciatus. These results show that this chitinase can synergize the toxicity of the binary toxin against mosquitoes and thus may be useful in managing mosquito resistance to B. sphaericus.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Cai
authors.forename: Jianpin
authors.initials: J
authors.lastname: Yan
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Hu
authors.forename: Bei
authors.initials: B
authors.lastname: Han
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000818:Animals; D001407:Bacillus / Q000235:genetics / Q000378:metabolism*; D002688:Chitinases / Q000235:genetics / Q000378:metabolism*; D003465:Culex / Q000382:microbiology*; D007700:Kinetics; D010572:Pest Control, Biological / Q000379:methods*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D011993:Recombinant Fusion Proteins; D002688:Chitinases