Comparative study of central corneal thickness measurement with slit-lamp optical coherence tomography and visante optical coherence tomography.
OBJECTIVE
To evaluate the repeatability and reproducibility of central corneal thickness (CCT) measurements obtained by 2 anterior segment optical coherence tomography (OCT) imaging systems and to examine their agreements with ultrasound pachymetry.


METHODS
Observational cross-sectional study.


METHODS
Fifty eyes from 50 healthy normal subjects were recruited.


METHODS
In one randomly selected eye in each subject, CCT was measured by slit-lamp OCT (SLOCT), Visante OCT, and ultrasound pachymetry. For anterior segment OCT measurements, both automatic and manual CCTs were obtained. Twenty-five of the 50 subjects were invited for 2 more visits within a week to evaluate repeatability and reproducibility of CCT measurement.


METHODS
Central corneal thickness measurement obtained by the 3 methods and their agreements. Intrasession and intersession within-subject standard deviation (S(w)), precision (1.96xS(w)), coefficient of variation (CV(w)) (100xS(w)/overall mean), and intraclass correlation coefficient (ICC) were calculated to evaluate repeatability and reproducibility.


RESULTS
Good repeatability and reproducibility were found for both automatic and manual CCT measurements obtained by SLOCT and Visante OCT. For intrasession repeatability, CV(w) and ICC values ranged between 0.9% and 1.2% and 0.96 and 0.98, respectively. For intersession reproducibility, the respective CV(w) and ICC values ranged between 1.2% and 1.4% and 0.94 and 0.96. Although no significant difference was found between automatic/manual SLOCT measurements and ultrasound pachymetry, automatic Visante OCT CCT (535.7+/-30.2 microm) was significantly less than CCT with ultrasound pachymetry (550.3+/-31.14 microm) (P<0.001). In contrast, manual Visante OCT measurement (558.8+/-32.8 microm) was slightly higher than ultrasound pachymetry (P<0.001). Nevertheless, SLOCT and Visante OCT measurement of CCT had 95% limits of agreement comparable to that of ultrasound pachymetry. The best agreement was observed in the manual SLOCT measurement (95% limits of agreement between -15.5 and 11.7 microm).


CONCLUSIONS
Both SLOCT and Visante OCT automatic and manual CCT measurements were reliable and showed comparable agreement with ultrasound pachymetry. Although the 2 anterior segment OCT imaging systems have similar design and working principles, clinicians should be aware of the differences in CCT measurement between the 2 anterior segment OCTs.
Li
Haitao
H
Department of Ophthalmology and Visual Sciences, Chinese University of Hong Kong, Hong Kong, China.
Leung
Christopher Kai Shun
CK
Wong
Lee
L
Cheung
Carol Yim Lui
CY
Pang
Chi Pui
CP
Weinreb
Robert Neal
RN
Lam
Dennis Shun Chiu
DS
D000328:Adult; D003315:Cornea / Q000033:anatomy & histology*; D003430:Cross-Sectional Studies; D003941:Diagnostic Techniques, Ophthalmological; D006801:Humans; D007368:Interferometry; D008027:Light; D033401:Microscopy, Acoustic; D015203:Reproducibility of Results; D041623:Tomography, Optical Coherence / Q000379:methods*