title: Side population cells expressing ABCG2 in human adult dental pulp tissue.
abstract: OBJECTIVE
  To investigate the presence of side population (SP) cells by the Hoechst exclusion method in human adult dental pulp tissue.
  
  
  METHODS
  Human adult dental pulp-derived cells were generated from third molar teeth. The cells were stained with Hoechst 33342 and sorted into SP cells or non-SP cells [main population (MP) cells]. Both cell types were compared with cell growth and RT-PCR analyses.
  
  
  RESULTS
  SP cells that express ABCG2, Nestin, Notch-1 and alpha-smooth muscle actin were found at frequencies ranging from 0.67% to 1.02%. This SP profile disappeared in the presence of verapamil. These SP cells expressed dentine sialophosphoprotein and dentine matrix protein-1 when cultured in osteogenic medium.
  
  
  CONCLUSIONS
  Human adult dental pulp tissue contains SP cells that differentiate into odontoblast-like cells.
authors.affiliation: Tooth Regeneration, Division of Stem Cell Engineering, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan. honda-m@ims.u-tokyo.ac.jp
authors.forename: M J
authors.initials: MJ
authors.lastname: Honda
authors.forename: F
authors.initials: F
authors.lastname: Nakashima
authors.forename: K
authors.initials: K
authors.lastname: Satomura
authors.forename: Y
authors.initials: Y
authors.lastname: Shinohara
authors.forename: S
authors.initials: S
authors.lastname: Tsuchiya
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Ueda
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis* / Q000494:pharmacology; D000199:Actins / Q000096:biosynthesis; D000293:Adolescent; D000328:Adult; D001562:Benzimidazoles; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005456:Fluorescent Dyes; D015870:Gene Expression; D006801:Humans; D007381:Intermediate Filament Proteins / Q000096:biosynthesis; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D064231:Nestin; D009804:Odontoblasts / Q000378:metabolism*; D010750:Phosphoproteins / Q000096:biosynthesis; D051881:Receptor, Notch1 / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism*; D014700:Verapamil / Q000494:pharmacology; D000099311:Dentin Sialophosphoprotein
chemical_list: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D000199:Actins; D001562:Benzimidazoles; D016326:Extracellular Matrix Proteins; D005456:Fluorescent Dyes; D007381:Intermediate Filament Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D064231:Nestin; D010750:Phosphoproteins; D051881:Receptor, Notch1; D012795:Sialoglycoproteins; D014700:Verapamil; D000099311:Dentin Sialophosphoprotein; C116614:ABCG2 protein, human; C513309:DMP1 protein, human; C577484:NES protein, human; C017807:bisbenzimide ethoxide trihydrochloride