Visual DNA -- identification of DNA sequence variations by bead trapping.
In this paper we describe a method that uses the nearly covalent strength biotin-streptavidin interaction to attach a paramagnetic bead of micrometer size to a DNA molecule of nanometer size, scaling up the spatial size of a query DNA strand by a factor of 1000, making it visible to the human eye. The use of magnetic principles enables rapid binding and washing of detector beads, facilitating a readout of amplified DNA sequences in a few minutes. Here we exemplify the method on mitochondrial DNA variations using an array platform. Visual identification and documentation can be performed with an ordinary mobile phone equipped with a built-in camera.
Ståhl
Patrik L
PL
Department of Gene Technology, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm, Sweden.
Gantelius
Jesper
J
Natanaelsson
Christian
C
Ahmadian
Afshin
A
Andersson-Svahn
Helene
H
Lundeberg
Joakim
J
D000818:Animals; D001710:Biotin; D004247:DNA / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D004285:Dogs; D005821:Genetic Techniques; D014644:Genetic Variation; D008280:Magnetics; D008863:Microspheres; D036103:Nanotechnology; D020411:Oligonucleotide Array Sequence Analysis; D017422:Sequence Analysis, DNA / Q000379:methods*; D019809:Streptavidin; D014584:User-Computer Interface
D004272:DNA, Mitochondrial; D004277:DNA, Single-Stranded; D001710:Biotin; D004247:DNA; D019809:Streptavidin