Kinetic study of the activation of banana juice enzymatic browning by the addition of maltosyl-beta-cyclodextrin.
In recent years, the use of cyclodextrins (CDs) as antibrowning agents in fruit juices has received growning attention. However, there has been no detailed study of the behavior of these molecules as substances, which can lead to the darkening of foods. In this paper, when the color of fresh banana juice was evaluated in the presence of different CDs, the evolution of several color parameters was the opposite of that observed in other fruit juices. Moreover, a kinetic model based on the complexation by CDs of the natural browning inhibitors present in banana is developed for the first time to clarify the enzymatic browning activation of banana juice. Finally, the apparent complexation constant between the natural polyphenoloxidase inhibitors present in banana juice and maltosyl-beta-CD was calculated (Kci = 27.026 +/- 0.212 mM (-1)).
López-Nicolás
José M
JM
Department of Biochemistry and Molecular Biology-A, Faculty of Biology, University of Murcia, Campus de Espinardo, 30071 Murcia, Spain. josemln@um.es
Pérez-López
Antonio J
AJ
Carbonell-Barrachina
Angel
A
García-Carmona
Francisco
F
D001628:Beverages / Q000032:analysis*; D004156:Catechol Oxidase / Q000378:metabolism; D003505:Cyclodextrins / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D007700:Kinetics; D015416:Maillard Reaction / Q000187:drug effects*; D008320:Maltose / Q000031:analogs & derivatives* / Q000494:pharmacology; D028521:Musa / Q000201:enzymology*
D003505:Cyclodextrins; C433303:maltosyl-cyclodextrin; D008320:Maltose; D004156:Catechol Oxidase