Increasing the agglutination reaction in slide test for weak B blood group by gold nanoparticle solution: the first world report.
OBJECTIVE
Blood group analysis is a basic immunohematological test. It should be noted that A, B, O and AB have various subgroups. Here, the authors used the advanced nanomedicine technique for modification of the basic blood group test for detection of weak B subgroup. Of interest, it can be seen that gold nanoparticle can help increase the agglutination reaction.


METHODS
The preparation of gold nanoparticle solution in this study was according to the standard method reported by Frenkel et al. The author performed an experiment on several conditions on weak B3 blood group sample, Anti B and gold nanoparticle solution.


RESULTS
In this study, the author found that more agglutination can be detected in the system of antigen, antibody and gold nanoparticle than in the system of only system of antigen and antibody.


CONCLUSIONS
This application can help diagnosis of weak blood group and can be the alternative way for the blood group test. This is the first world report on this application.
Wiwanitkit
Viroj
V
Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. wviroj@yahoo.com
Seereemaspun
Amornpun
A
Rojanathanes
Rojanarit
R
D000017:ABO Blood-Group System / Q000032:analysis*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D006046:Gold; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D053768:Metal Nanoparticles
D000017:ABO Blood-Group System; D006046:Gold