Folate fortification of rice by metabolic engineering.
Rice, the world's major staple crop, is a poor source of essential micronutrients, including folates (vitamin B9). We report folate biofortification of rice seeds achieved by overexpressing two Arabidopsis thaliana genes of the pterin and para-aminobenzoate branches of the folate biosynthetic pathway from a single locus. We obtained a maximal enhancement as high as 100 times above wild type, with 100 g of polished raw grains containing up to four times the adult daily folate requirement.
Storozhenko
Sergei
S
Unit Plant Hormone Signalling and Bio-imaging, Department of Molecular Genetics, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
De Brouwer
Veerle
V
Volckaert
Maarten
M
Navarrete
Oscar
O
Blancquaert
Dieter
D
Zhang
Guo-Fang
GF
Lambert
Willy
W
Van Der Straeten
Dominique
D
D010129:4-Aminobenzoic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics; D005492:Folic Acid / Q000096:biosynthesis* / Q000235:genetics*; D005527:Food, Fortified; D017343:Genes, Plant; D005818:Genetic Engineering; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011622:Pterins / Q000378:metabolism
D011622:Pterins; D005492:Folic Acid; D010129:4-Aminobenzoic Acid