Synaptic localization of seizure-induced matrix metalloproteinase-9 mRNA.
The phenomenon of dendritic transport and local translation of mRNA is considered to be one of the most fundamental mechanisms underlying long-term synaptic plasticity. Matrix metalloproteinase 9 (gelatinase B) (MMP-9) is a matrix metalloproteinase implicated in synaptic long-term potentiation and hippocampus-dependent memory. It was recently shown to be prominently up-regulated in the hippocampal dentate gyrus (DG) upon kainate-mediated seizures. Here, using a high resolution nonradioactive in situ hybridization at the light- and electron-microscopic levels, as well as subcellular fractionation, we provide evidence that in the rat hippocampus, MMP-9 mRNA is associated with dendrites and dendritic spines bearing asymmetric (excitatory) synapses. Moreover we observe that after kainate treatment the number of dendrites and synapses containing MMP-9 mRNA increases markedly. Our results indicate that we are observing the phenomenon of dendritic transport of seizure-induced MMP-9 mRNA.
Konopacki
F A
FA
Department of Neurophysiology, Nencki Institute of Experimental Biology, 02-093 Warsaw, Poland.
Rylski
M
M
Wilczek
E
E
Amborska
R
R
Detka
D
D
Kaczmarek
L
L
Wilczynski
G M
GM
D000818:Animals; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D007608:Kainic Acid; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics* / Q000378:metabolism; D016253:Microscopy, Immunoelectron / Q000379:methods; D016761:Oncogene Proteins v-fos / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D009418:S100 Proteins / Q000378:metabolism; D013226:Status Epilepticus / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology*; D013569:Synapses / Q000378:metabolism* / Q000648:ultrastructure; D013574:Synaptosomes / Q000378:metabolism / Q000648:ultrastructure; D013997:Time Factors
D016761:Oncogene Proteins v-fos; D012333:RNA, Messenger; D009418:S100 Proteins; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D007608:Kainic Acid