title: Hysteroscopic sterilization with occlusion of sheep uterine tube using n-butyl-2-cyanoacrylate adhesive.
abstract: OBJECTIVE
  To evaluate the fertility and analyze the macroscopic, microscopic and morphometric aspects of sheep uterine tube sterilization with a hysteroscopically insert of n-butyl-2-cyanoacrylate adhesive.
  
  
  METHODS
  12 adult sheep, with one previous pregnancy, were distributed as follows: group L (n=3) subjected to laparotomy and Pomeroy uterine tube ligation, group S (n=3) subjected to hysteroscopic application of saline solution in tube isthmus and group AD(n=6), that was subjected to hysteroscopic application of 0.5 ml of n-2-butil-cyanoacrylate in tube isthmus. They were mated with fertile males for ninety days. The non pregnant sheep, at the 90th day, were subjected to laparotomy with uterus and tubes uterine resection. The fragments of uterine tubes were fixated in 10% formalin and processes for histology evaluated, and slices dyes for H.E. Data were evaluated by Wilcoxon and Mann-Whitney and Fisher's exact test.
  
  
  RESULTS
  All sheep from groups L and AD did not get pregnant (0%) in contrast with sheep from group S (100%); the adhesive remained integral in the uterine tube lumen. The percentual of adherences (66.6%) and fibrosis responses (100%) was significantly higher in the group L than group AD (0%) (p<0.01). The diameter of the caudal tube in group AD (2652.15 +/- 45.76 mm) was significantly wider than that of the group L (1868.27 +/- 56.11* microm) (p < 0.05).
  
  
  CONCLUSIONS
  The hysteroscopic insertion of cyanoacrylate in the uterine tube lumen of sheep was effective to obstruct the uterine tube and to promote the sterilization.
authors.affiliation: Division of Gynecology, Regional Integrated University of High Uruguay and Missions, Rio Grande do Sul, Brazil. bigolin@itake.net.br
authors.forename: Sergio
authors.initials: S
authors.lastname: Bigolin
authors.forename: Djalma José
authors.initials: DJ
authors.lastname: Fagundes
authors.forename: Henri Chaplin
authors.initials: HC
authors.lastname: Rivoire
authors.forename: Anna Tereza Negrini
authors.initials: AT
authors.lastname: Fagundes
authors.forename: Ricardo
authors.initials: R
authors.lastname: Simões
authors.forename: Manuel de Jesus
authors.initials: Mde J
authors.lastname: Simões
mesh_terms: D000818:Animals; D004659:Enbucrilate / Q000031:analogs & derivatives* / Q000627:therapeutic use; D005187:Fallopian Tubes / Q000033:anatomy & histology; D005260:Female; D005298:Fertility / Q000187:drug effects* / Q000502:physiology; D005355:Fibrosis / Q000209:etiology; D015907:Hysteroscopy; D023421:Models, Animal; D011247:Pregnancy; D012756:Sheep; D012965:Sodium Chloride / Q000627:therapeutic use; D013246:Sterilization, Tubal / Q000009:adverse effects / Q000379:methods*; D000267:Tissue Adhesions / Q000209:etiology; D014014:Tissue Adhesives / Q000627:therapeutic use*
chemical_list: D014014:Tissue Adhesives; D012965:Sodium Chloride; D004659:Enbucrilate