title: Effect of the diazepam-binding inhibitor-derived peptide, octadecaneuropeptide, on food intake in goldfish.
abstract: An endogenous ligand of central-type benzodiazepine receptors (CBR), the endozepine octadecaneuropeptide (ODN), is a very potent inhibitor of food intake in rodents. Although endozepines have been localized and characterized in the trout hypothalamus, so far, the action of these neuropeptides on feeding behavior has never been investigated in fish. In the present study, we have examined the effect of i.c.v. administration of synthetic rat ODN, its C-terminal octapeptide (OP) and the head-to-tail cyclic analog cyclo(1-8)OP (cOP) on feeding behavior in the goldfish model. i.c.v. injection of graded doses of ODN (2.5-10 pmol/g body weight (BW)) induced a dose-dependent inhibition of food intake, a significant decrease in cumulative food intake during the 60-min period after feeding being observed at doses of 5 and 10 pmol/g BW. The inhibitory effect of a 10 pmol/g BW dose of ODN on food consumption (-39%) was mimicked by an equimolar dose of OP (-42%) and cOP (-53%). The food intake-suppressing activity of ODN (10 pmol/g BW) was not affected by pre-injection of the CBR antagonist flumazenil (200 pmol/g BW). In contrast, the anorexigenic effect of ODN (10 pmol/g BW) was totally suppressed by a selective antagonist of metabotropic endozepine receptors, cyclo(1-8)[dLeu(5)]OP. These data indicate that, in goldfish as in rodents, ODN is a potent inhibitor of food consumption, and that the anorexigenic effect of ODN is not mediated through CBR but through the metabotropic endozepine receptor.
authors.affiliation: Laboratory of Regulatory Biology, Graduate School of Science and Engineering, University of Toyama, 3190-Gofuku, Toyama 930-8555, Japan. kmatsuda@sci.u-toyama.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Wada
authors.forename: T
authors.initials: T
authors.lastname: Miura
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
authors.forename: S I
authors.initials: SI
authors.lastname: Shimakura
authors.forename: M
authors.initials: M
authors.lastname: Uchiyama
authors.forename: J
authors.initials: J
authors.lastname: Leprince
authors.forename: M C
authors.initials: MC
authors.lastname: Tonon
authors.forename: H
authors.initials: H
authors.lastname: Vaudry
mesh_terms: D000818:Animals; D001069:Appetite Regulation / Q000187:drug effects* / Q000502:physiology; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D026261:Diazepam Binding Inhibitor / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects / Q000502:physiology; D005247:Feeding Behavior / Q000187:drug effects / Q000502:physiology; D005260:Female; D005442:Flumazenil / Q000494:pharmacology; D018757:GABA Modulators / Q000494:pharmacology; D006054:Goldfish; D008024:Ligands; D008297:Male; D009479:Neuropeptides / Q000737:chemistry / Q000494:pharmacology*; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000378:metabolism
chemical_list: D026261:Diazepam Binding Inhibitor; D018757:GABA Modulators; D008024:Ligands; D009479:Neuropeptides; D010446:Peptide Fragments; D011963:Receptors, GABA-A; C067529:diazepam binding inhibitor (33-50); D005442:Flumazenil