Preparation and function of composite asymmetric chitosan/CM-chitosan membrane.
A novel composite asymmetric chitosan/CM-chitosan membrane (C-P-C) was prepared, the top-layer was chitosan (CS), the intermediate was PVA, and the substrate was carboxymethyl chitosan (CM-CS). C-P-C membrane had capability in mechanical strength, light transparence, vapor permeability, and wound skin joining. The CS and CM-CS in C-P-C membrane were selected by series independent experiments, respectively. CS (MW 90,000 Da) had the highest antibacterial activity for E.coli. CM-CS had biocompatibility, no cytotoxicity, and had the activity of promoting growth of human skin fibroblast and inhibiting the growth of keloid fibroblast. The normal skin fibroblast can growth on the CM-CS surface of C-P-C, and have no conglomeration in higher cell density, and the keloid fibroblast could not growth on CM-CS surface of C-P-C. The animal experiment demonstrated that wound, covered with the C-P-C membrane, was hemostatic, healing quickly and had histocompatibility. The results indicated that the C-P-C membrane could be used as dressing of skin repair, and had the potential in promoting wound healing and inhibiting the keloid formation.
Pang
Hong Tao
HT
The Division of Life Science and Technology, College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao 266003, P.R. China.
Chen
Xi Guang
XG
Ji
Qiu Xia
QX
Zhong
De Yu
de Y
D020341:Absorbable Implants; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry*; D015169:Colony Count, Microbial; D003188:Composite Resins / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D008297:Male; D008567:Membranes, Artificial; D051379:Mice; D008826:Microbial Sensitivity Tests; D008968:Molecular Conformation
D000900:Anti-Bacterial Agents; D003188:Composite Resins; D008567:Membranes, Artificial; C514968:carboxymethyl-chitosan; D048271:Chitosan