title: Involvement of GABA(A) receptors in the neuroprotective effect of theanine on focal cerebral ischemia in mice.
abstract: We investigated the involvement of gamma-aminobutyric acid(A) (GABA(A)) receptors in the neuroprotective effect of gamma-glutamylethylamide (theanine), a component of Japanese green tea, following a 4-h middle cerebral artery (MCA) occlusion in mice. Theanine (1 mg/kg) reduced the size of the cerebral infarct and alterations of NeuN, GFAP, and Iba 1 expression levels at 24 h after MCA occlusion. This neuroprotective effect of theanine was prevented by bicuculline (GABA(A)-receptor antagonist, 10 mg/kg) but not 3-mercaptopropionic acid (glutamate decarboxylase inhibitor). These results suggest that the neuroprotective effect of theanine is mediated, at least in part, by GABA(A) receptors.
authors.affiliation: Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Egashira
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Hayakawa
authors.forename: Megumi
authors.initials: M
authors.lastname: Osajima
authors.forename: Kenichi
authors.initials: K
authors.lastname: Mishima
authors.forename: Katsunori
authors.initials: K
authors.lastname: Iwasaki
authors.forename: Ryozo
authors.initials: R
authors.lastname: Oishi
authors.forename: Michihiro
authors.initials: M
authors.lastname: Fujiwara
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy*; D002135:Calcium-Binding Proteins / Q000187:drug effects / Q000378:metabolism; D028241:Camellia sinensis / Q000737:chemistry; D004268:DNA-Binding Proteins; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000187:drug effects / Q000378:metabolism; D005971:Glutamates / Q000494:pharmacology*; D020244:Infarction, Middle Cerebral Artery; D008297:Male; D051379:Mice; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009687:Nuclear Proteins / Q000187:drug effects / Q000378:metabolism; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000378:metabolism; D013662:Tea / Q000737:chemistry
chemical_list: C491049:Aif1 protein, mouse; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D005971:Glutamates; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; C492380:NeuN protein, mouse; D018696:Neuroprotective Agents; D009687:Nuclear Proteins; D011963:Receptors, GABA-A; D013662:Tea; C026166:theanine