title: Gene expression variability in subcutaneous and omental adipose tissue of obese men.
abstract: We investigated interindividual variability in gene expression in abdominal subcutaneous (SC) and omental (OM) adipose tissue of 10 massively obese men. Affymetrix human U133A microarrays were used to measure gene expression levels. A total of 6811 probesets generated significant signal in both depots in all samples. Interindividual variability in gene expression was rather low, with more than 90% of transcripts showing a coefficient of variation (CV) lower than 23.6% and 21.7% in OM and SC adipose tissues, respectively. The distributions of CV were similar between the two fat depots. A set of highly variable genes was identified for both tissues on the basis of a high CV and elevated gene expression level. Among the set of highly regulated genes, 18 transcripts were involved in lipid metabolism and 28 transcripts were involved in cell death for SC and OM samples, respectively. In conclusion, gene expression interindividual variability was rather low and globally similar between fat compartments, and the adipose tissue transcriptome appeared as relatively stable, although specific pathways were found to be highly variable in SC and OM depots.
authors.affiliation: Molecular Endocrinology and Oncology Research Center, Laval University Medical Research Center, Québec, Canada.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yohan
authors.initials: Y
authors.lastname: Bossé
authors.forename: Picard
authors.initials: P
authors.lastname: Marceau
authors.forename: Simon
authors.initials: S
authors.lastname: Biron
authors.forename: Stephan
authors.initials: S
authors.lastname: Lebel
authors.forename: Denis
authors.initials: D
authors.lastname: Richard
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Vohl
authors.forename: André
authors.initials: A
authors.lastname: Tchernof
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D016923:Cell Death / Q000235:genetics; D015870:Gene Expression; D014644:Genetic Variation; D006801:Humans; D050356:Lipid Metabolism / Q000235:genetics; D008297:Male; D046228:Microarray Analysis; D009765:Obesity; D009852:Omentum; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050151:Subcutaneous Fat / Q000378:metabolism*