Adiponectin deficiency suppresses ABCA1 expression and ApoA-I synthesis in the liver.
Plasma high density lipoprotein (HDL)-cholesterol levels are inversely correlated with the incidence of cardiovascular diseases. HDL is mainly assembled in the liver through the ATP-binding cassette transporter (ABCA1) pathway. In humans, plasma HDL-cholesterol levels are positively correlated with plasma adiponectin (APN) concentrations. Recently, we reported that APN enhanced apolipoprotein A-I (apoA-I) secretion and ABCA1 expression in HepG2 cells. In the present study, we investigated HDL assembly in APN-knockout (KO) mice. The apoA-I protein levels in plasma and liver were reduced in APN-KO mice compared with wild-type-mice. The ABCA1 expression in liver was also decreased in APN-KO mice. APN deficiency might cause the impaired HDL assembly by decreasing ABCA1 expression and apoA-I synthesis in the liver.
Oku
Hiroyuki
H
Department of Cardiovascular Medicine, Osaka University, Graduate School of Medicine, 2-2, Yamadaoka, Suita, Osaka 565-0871, Japan.
Matsuura
Fumihiko
F
Koseki
Masahiro
M
Sandoval
Jose C
JC
Yuasa-Kawase
Miyako
M
Tsubakio-Yamamoto
Kazumi
K
Masuda
Daisaku
D
Maeda
Norikazu
N
Ohama
Tohru
T
Ishigami
Masato
M
Nishida
Makoto
M
Hirano
Ken-ichi
K
Kihara
Shinji
S
Hori
Masatsugu
M
Shimomura
Iichiro
I
Yamashita
Shizuya
S
D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D052242:Adiponectin / Q000235:genetics / Q000502:physiology*; D000818:Animals; D016632:Apolipoprotein A-I / Q000097:blood / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000097:blood / Q000378:metabolism*; D008079:Lipoproteins, VLDL / Q000097:blood; D008099:Liver / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout
C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D052242:Adiponectin; D016632:Apolipoprotein A-I; D008075:Lipoproteins, HDL; D008079:Lipoproteins, VLDL