Hypoxia: dual effect on the expression of transferrin receptor in human melanoma A375 cell line.
BACKGROUND
Over-expression of transferrin receptor (TfR) is a common feature of human malignancies. Therapeutic strategies designed to interfere with tumor iron metabolism have targeted TfR. In previous studies, our laboratory successfully constructed the human-mouse chimeric antibody against TfR and it displayed a tumor-specificity distribution, and has a strong anti-tumor effect. We also found that there were still some limitations to anti-tumor effect in vivo. Oxygen and iron have a very tight relationship, and hypoxia is considered a fundamentally important characteristic of the tumor microenvironment. To exploit the target molecule TfR more rationally and effectively, we were prompted to explore TfR expression under hypoxia.


OBJECTIVE
To examine the expressing alteration of TfR of human melanoma A375 cell line under hypoxia at various time points (0, 12, 24, 36, 48 and 60 h).


METHODS
The expressing alteration of TfR of A375 cell line under hypoxia at various time points (0, 12, 24, 36, 48 and 60 h) was assayed by flow cytometry, real-time RT-PCR and Western blot.


RESULTS
Hypoxia has dual effect on the expression of TfR in human melanoma A375 cell line.


CONCLUSIONS
These findings may have important implications for more rational, individualized gene-based therapy using TfR as target receptor in melanoma.
Tao
Juan
J
Department of Dermatology, Affiliated Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Liu
Ye-qiang
YQ
Li
Yan
Y
Peng
Ji-ling
JL
Li
Li
L
Liu
Jing
J
Shen
Xin
X
Shen
Guan-xin
GX
Tu
Ya-ting
YT
D017209:Apoptosis / Q000502:physiology; D015687:Cell Hypoxia / Q000502:physiology*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D010100:Oxygen / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011990:Receptors, Transferrin / Q000235:genetics / Q000378:metabolism*; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051034:Inhibitor of Apoptosis Proteins; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D051028:bcl-2-Associated X Protein; D007501:Iron; D010100:Oxygen