Glut-1 antibodies induce growth arrest and apoptosis in human cancer cell lines.
Glucose transporters (Gluts) facilitate glucose uptake and tumors frequently over express the Gluts, especially Glut-1. Breast cancer and lung cancer (NSCLC) cell lines were incubated with anti-Glut-1 antibodies alone or with cisplatin, paclitaxel or gefitinib. Inhibition of proliferation and apoptosis was assessed. Antibodies to Glut-1 inhibited proliferation by 50% and 75% in the tested NSCLC and breast cancer cell lines, respectively. They also potentiate the anti-proliferative effects of cisplatin, paclitaxel and gefitinib. Our results indicate that anti-Glut-1 antibodies inhibit proliferation and induce apoptosis in the evaluated cell lines and provide preliminary evidence of their anti-tumor activity.
Rastogi
Shipra
S
Drug Discovery Program, H Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA.
Banerjee
Sarmistha
S
Chellappan
Srikumar
S
Simon
George R
GR
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001692:Biological Transport / Q000187:drug effects; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology; D004357:Drug Synergism; D000077156:Gefitinib; D005947:Glucose / Q000493:pharmacokinetics; D051272:Glucose Transporter Type 1 / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D017239:Paclitaxel / Q000494:pharmacology; D011799:Quinazolines / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D051272:Glucose Transporter Type 1; D011799:Quinazolines; D005947:Glucose; D017239:Paclitaxel; D002945:Cisplatin; D000077156:Gefitinib