title: Polyhemoglobin-fibrinogen: a novel oxygen carrier with platelet-like properties in a hemodiluted setting.
abstract: Polyhemoglobin (polyHb) is currently being assessed in phase III trials under various formulations. At present, none contain clotting factors or platelet substitutes to aid in hemostasis. We have prepared a novel blood substitute that is an oxygen carrier with platelet-like activity. This is formed by crosslinking fibrinogen to hemoglobin to form polyhemoglobin-fibrinogen (polyHb-Fg). This was studied and compared to polyHb for its effect on coagulation both in vitro and in vivo. In the in vitro experiments, PolyHb-Fg showed similar clotting times as whole blood, whereas polyHb showed significantly higher clotting times. This result was confirmed in in vivo experiments using an exchange transfusion rat-model. Using PolyHb, exchange transfusion of 80% or more increased the normal clotting time (1-2 mins) to > 10 mins. Partial clots formed with PolyHb did not adhere to the tubing wall. With PolyHb-Fg, a normal clotting time is maintained, even with 98% exchange transfusion.
authors.affiliation: Artificial Cells and Organs Research Centre, Department of Physiology, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
authors.forename: Naomi S W
authors.initials: NS
authors.lastname: Wong
authors.forename: Thomas M S
authors.initials: TM
authors.lastname: Chang
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000502:physiology; D001802:Blood Substitutes / Q000138:chemical synthesis*; D005078:Exchange Transfusion, Whole Blood / Q000379:methods; D005340:Fibrinogen / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D006438:Hemodilution / Q000009:adverse effects; D006454:Hemoglobins / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014914:Whole Blood Coagulation Time
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; C037158:polyhemoglobin; C525511:polyhemoglobin-fibrinogen; D005340:Fibrinogen