Production of L2 lipase by Bacillus sp. strain L2: nutritional and physical factors.
A thermophilic bacterium, Bacillus sp. strain L2 was isolated from a hot spring in Perak, Malaysia. An extracellular lipase activity was detected through plate and broth assays at 70 degrees C after 28 h of incubation. The L2 lipase production was growth dependent as revealed by a number of factors affecting the secretion of extracelullar lipase. As for nutritional factors, casamino acids, trehalose, Ca(2+) and Tween 60 were found to be more effective for lipase production. The optimum physical condition for L2 lipase production was obtained at 70 degrees C after 28 h of cultivation time, at pH 7.0, 150 rpm of agitation rate and 1% of starting inoculum size. The activity staining of crude L2 lipase revealed a clearing zone at 39 kDa.
Shariff
Fairolniza Mohd
FM
Enzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Leow
Thean Chor
TC
Mukred
A D
AD
Salleh
Abu Bakar
AB
Basri
Mahiran
M
Rahman
Raja Noor Zaliha Raja Abd
RN
D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D045482:Hot Springs / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D008049:Lipase / Q000096:biosynthesis* / Q000302:isolation & purification; D008296:Malaysia; D013696:Temperature
D008049:Lipase