title: Activation of T-cells from allergic patients and volunteers by p-phenylenediamine and Bandrowski's base.
abstract: Allergic contact dermatitis is commonly associated with exposure to p-phenylenediamine. The aim of this study was to determine whether p-phenylenediamine (PPD) and/or Bandrowski's base (BB) stimulate T cells from allergic patients and volunteers, and to explore the relationship between T-cell immunogenicity and allergy. Lymphocytes from allergic patients proliferated with PPD and BB (n=8). Lymphocytes from 14/16 non-allergic individuals also proliferated following stimulation, but only with BB; cord blood lymphocytes failed to respond (n=6). Glutathione, which prevented BB formation, but not binding of PPD to cells and serum, did not prevent p-phenylenediamine-specific stimulation of patient lymphocytes. T-cell clones generated from allergic patients were stimulated separately with PPD and BB, while clones from volunteers proliferated with BB alone. Patient and volunteer clones secreted IL-4, IL-5, IL-13, TNF-alpha, MIP-1alpha, MIP-1beta, and RANTES. These data show that activation of T lymphocytes from allergic individuals alone with PPD represents an important discrimination between allergic and non-allergic groups. BB-specific T cells are found in both allergic patients and volunteers, but not in cord blood. Their presence seems to reflect an acquired immune response, which is not translated into an allergic reaction.
authors.affiliation: Department of Pharmacology, The University of Liverpool, Liverpool, UK.
authors.forename: Eve Marie
authors.initials: EM
authors.lastname: Coulter
authors.forename: Claire
authors.initials: C
authors.lastname: Jenkinson
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.forename: John
authors.initials: J
authors.lastname: Farrell
authors.forename: Brian
authors.initials: B
authors.lastname: Foster
authors.forename: Andrew
authors.initials: A
authors.lastname: Smith
authors.forename: Carolann
authors.initials: C
authors.lastname: McGuire
authors.forename: Camilla
authors.initials: C
authors.lastname: Pease
authors.forename: David
authors.initials: D
authors.lastname: Basketter
authors.forename: Clodagh
authors.initials: C
authors.lastname: King
authors.forename: Peter Simon
authors.initials: PS
authors.lastname: Friedmann
authors.forename: Munir
authors.initials: M
authors.lastname: Pirmohamed
authors.forename: Brian Kevin
authors.initials: BK
authors.lastname: Park
authors.forename: Dean J
authors.initials: DJ
authors.lastname: Naisbitt
mesh_terms: D000328:Adult; D000368:Aged; D015704:CD4 Antigens / Q000187:drug effects / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D017449:Dermatitis, Allergic Contact / Q000276:immunology*; D005260:Female; D006202:Hair Dyes / Q000009:adverse effects* / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D010655:Phenylenediamines / Q000276:immunology* / Q000494:pharmacology
chemical_list: D015704:CD4 Antigens; D016207:Cytokines; D006202:Hair Dyes; D010655:Phenylenediamines; C051597:Bandrowski's base; C029728:4-phenylenediamine