Profiles of a pathogenic Entamoeba histolytica-like variant with variations in the nucleotide sequence of the small subunit ribosomal RNA isolated from a primate (De Brazza's guenon).
A pathogenic Entamoeba histolytica-like variant (JSK2004) with genetic variations and a novel isoenzyme pattern was isolated from a De Brazza's guenon. A homology of 99.1% was found between the clones of E. histolytica (HM-1:IMSS) and JSK2004 in the 1,893 nucleotide bases of the small subunit rRNA (SSU-rRNA) gene. The DNA of the pathogenic amoeba species was also extracted from two sterile liver abscesses during the autopsies of an Abyssinian colobus and a Geoffroy's spider monkey occurring in the same institution in which JSK2004 was isolated, and the homology of the nucleotide sequences in the SSU-rRNA gene of the DNAs was identical to that of JSK2004.
Suzuki
Jun
J
Division of Clinical Microbiology, Department of Microbiology, Tokyo Metropolitan Institute of Public Health, 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
Kobayashi
Seiki
S
Murata
Rie
R
Yanagawa
Yoshitoki
Y
Takeuchi
Tsutomu
T
D000818:Animals; D001483:Base Sequence; D002521:Cercopithecus; D016054:DNA, Protozoan / Q000235:genetics; D004747:Entamoeba / Q000145:classification / Q000235:genetics*; D004748:Entamoeba histolytica / Q000145:classification / Q000235:genetics; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000469:parasitology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016053:RNA, Protozoan / Q000235:genetics; D012335:RNA, Ribosomal / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D016054:DNA, Protozoan; D016053:RNA, Protozoan; D012335:RNA, Ribosomal