title: ZNF307, a novel zinc finger gene suppresses p53 and p21 pathway.
abstract: We have cloned a novel KRAB-related zinc finger gene, ZNF307, encoding a protein of 545aa. ZNF307 is conserved across species in evolution and is differentially expressed in human adult and fetal tissues. The fusion protein of EGFP-ZNF307 localizes in the nucleus. Transcriptional activity assays show ZNF307 suppresses transcriptional activity of L8G5-luciferase. Overexpressing ZNF307 in different cell lines also inhibits the transcriptional activities of p53 and p21. Moreover, ZNF307 works by reducing the p53 protein level and p53 protein reduction is achieved by increasing transcription of MDM2 and EP300. ZNF307 might suppress p53-p21 pathway through activating MDM2 and EP300 expression and inducing p53 degradation.
authors.affiliation: The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, Changsha, 410081 Hunan, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yuequn
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiongwei
authors.initials: X
authors.lastname: Fan
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Mo
authors.forename: Zequn
authors.initials: Z
authors.lastname: Wang
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Li
authors.forename: Zhaochu
authors.initials: Z
authors.lastname: Yin
authors.forename: Yun
authors.initials: Y
authors.lastname: Deng
authors.forename: Na
authors.initials: N
authors.lastname: Luo
authors.forename: Chuanbing
authors.initials: C
authors.lastname: Zhu
authors.forename: Mingyao
authors.initials: M
authors.lastname: Liu
authors.forename: Qian
authors.initials: Q
authors.lastname: Ma
authors.forename: Karen
authors.initials: K
authors.lastname: Ocorr
authors.forename: Wuzhou
authors.initials: W
authors.lastname: Yuan
authors.forename: Xiushan
authors.initials: X
authors.lastname: Wu
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D050881:E1A-Associated p300 Protein / Q000235:genetics; D019143:Evolution, Molecular; D005786:Gene Expression Regulation; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism; D009928:Organ Specificity; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics; D016283:Proto-Oncogene Proteins p21(ras) / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D016159:Tumor Suppressor Protein p53; C524688:ZKSCAN4 protein, human; D050881:E1A-Associated p300 Protein; C494360:EP300 protein, human; D051736:Proto-Oncogene Proteins c-mdm2; D016283:Proto-Oncogene Proteins p21(ras)