title: Isolation of Actinobacillus pleuropneumoniae serovar 15-like strain from a field case of porcine pleuropneumonia in Japan.
abstract: An Actinobacillus pleuropneumoniae strain isolated from a field case of porcine pleuropneumonia in Japan, was closely related to a reference strain of serovar 15, which is a newly proposed serovar according to an analysis of field isolates originating from Australia. The isolate had biological and biochemical properties consistent with A. pleuropneumoniae biovar 1, and reacted strongly to a rabbit antiserum raised against a reference strain of serovar 15 in an agar gel precipitation test. The nucleotide sequence of a hyper variable region in the 16S RNA gene of the isolate was identical to that of the reference strain of serovar 15. The isolate possessed A. pleuropneumoniae-RTX toxin (Apx) II, III, and IV genes, consistent with serovar 15. Its virulence in mice was lower than that of ApxI-bearing strains but higher than that of other ApxIII-bearing strains. This is the first report describing the isolation of A. pleuropneumoniae serovar 15-like strain from a country or region other than Australia.
authors.affiliation: Nippon Institute for Biological Science, 9-2221-1 Shin-machi, Ome, Tokyo 198-0024, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Koyama
authors.forename: Ho
authors.initials: H
authors.lastname: To
authors.forename: Shinya
authors.initials: S
authors.lastname: Nagai
mesh_terms: D000189:Actinobacillus Infections / Q000382:microbiology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics; D001681:Biological Assay; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D051379:Mice; D011001:Pleuropneumonia / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S