Spectral study of the interaction of DNA with benzothiazolyl-benz-alpha-chromene.
Absorption and luminescence excitation and emission spectra of newly synthesized 2-(4-methylphenylimino)-3-(2 -benzothiazolyl)benz-alpha-chromene (BCBT) have been studied in the presence of various DNA concentrations. BCBT is characterized by the existence of two different fluorescent systems, exhibiting radiationless fluorescence resonance energy transfer between them. In the range of molar ratios of polynucleotide/dye concentrations from 0 to 50, BCBT preferentially intercalates into DNA due to its benz-alpha-chromene fragment, whereas the 2-benzothiazolyl fragment is responsible for fluorescence.
Sibirtsev
V S
VS
Mendeleyev Russian Research Institute for Metrology, St. Petersburg, 190005, Russia. vs1969r@mail.ru
Garabadzhiu
A V
AV
D001578:Benzopyrans / Q000138:chemical synthesis / Q000737:chemistry*; D052160:Benzothiazoles / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D007364:Intercalating Agents / Q000138:chemical synthesis / Q000737:chemistry*
C524649:2-(4-methylphenylimino)-3-(2 -benzothiazolyl)benz-alpha-chromene; D001578:Benzopyrans; D052160:Benzothiazoles; D005456:Fluorescent Dyes; D007364:Intercalating Agents; D004247:DNA