title: Identification of a metagenome-derived esterase with high enantioselectivity in the kinetic resolution of arylaliphatic tertiary alcohols.
abstract: 35 metagenome-derived esterases bearing a GGG(A)X motif were screened for activity and enantioselectivity in the hydrolysis of a range of tertiary alcohol acetates. Most of the active esterases showed little or no enantioselectivity in the hydrolysis of the terpinyl acetate, linalyl acetate and 3-methylpent-1-yn-3-yl acetate. However, one esterase showed excellent enantioselectivity (E > 100) in the kinetic resolution of 1,1,1-trifluoro-2-phenylbut-3-yn-2-yl acetate as confirmed by a preparative scale reaction.
authors.affiliation: Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Kourist
authors.forename: Sajja
authors.initials: S
authors.lastname: Hari Krishna
authors.forename: Jesal S
authors.initials: JS
authors.lastname: Patel
authors.forename: Flash
authors.initials: F
authors.lastname: Bartnek
authors.forename: Timothy S
authors.initials: TS
authors.lastname: Hitchman
authors.forename: David P
authors.initials: DP
authors.lastname: Weiner
authors.forename: Uwe T
authors.initials: UT
authors.lastname: Bornscheuer
mesh_terms: D000085:Acetates / Q000737:chemistry*; D004950:Esterases / Q000737:chemistry* / Q000235:genetics*; D015723:Gene Library; D016678:Genome; D007700:Kinetics
chemical_list: D000085:Acetates; D004950:Esterases