Determination of a relationship between chitinase activity and microbial diversity in chitin amended compost.
By using denaturing gradient gel electrophoresis (DGGE) and simultaneously measuring the enzymatic activity of chitinase, we could link genetic diversity of the indigenous microbial communities with chitinase activity in compost samples. A garden/park waste compost and a source separated organic household waste compost, showed different genetic diversity as measured by PCR-DGGE of total DNA extracted from the composts. The household waste compost had the highest chitinase activity. To increase chitinase activity, the two composts were amended with chitin. This addition induced a change in both the bacterial and fungal genetic diversity when compared to the non-amended compost samples. Likewise, both composts reacted to the addition of chitin with an increase in chitinase activity. Thus, a relationship between genetic diversity and chitinase activity was established for the composts in question. The N-mineralization in the household waste compost was apparently increased by the addition of chitin, while such an effect was not observed in the garden/park waste compost.
Poulsen
Pernille H B
PH
Department of Agricultural Sciences, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. pepo@life.ku.dk
Møller
Jacob
J
Magid
Jakob
J
D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D044822:Biodiversity; D001709:Biotechnology / Q000379:methods*; D002686:Chitin / Q000032:analysis*; D002688:Chitinases / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000737:chemistry; D017753:Ecosystem; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005658:Fungi / Q000378:metabolism; D009584:Nitrogen / Q000032:analysis; D016133:Polymerase Chain Reaction; D012037:Refuse Disposal / Q000379:methods; D012987:Soil; D012988:Soil Microbiology
D012987:Soil; D002686:Chitin; D004247:DNA; D002688:Chitinases; D009584:Nitrogen