title: Thiophilic interaction chromatography (TIC) of amyloid-beta protein precursor.
abstract: Neuritic plaques, one of the diagnostic characteristics of an AD, contain extracellular deposits of amyloid-beta (Abeta) derived from amyloid-beta protein precursor (AbetaPP). The objective of this study was to extract AbetaPP out of HEK293 cells and to purify it. Two procedures were chosen for purification of AbetaPP: Thiophilic Interaction Chromatography (TIC) and molecular sieving. Using Superdex 75, Superose 12, and Fractogel gel matrices, AbetaPP was isolated on HPLC. The chromatograms illustrate the purification of AbetaPP. Our method describes a new and elegant way for the extraction and purification of AbetaPP from HEK293 cell lines using thiophilic interaction chromatography (TIC).
authors.affiliation: Department of Cancer Biology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
authors.forename: Mustapha
authors.initials: M
authors.lastname: Bourhim
authors.forename: Tara
authors.initials: T
authors.lastname: Johnson
authors.forename: Derek
authors.initials: D
authors.lastname: Oyston
authors.forename: Thamarapu
authors.initials: T
authors.lastname: Srikrishnan
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002458:Cell Fractionation; D002478:Cells, Cultured; D002846:Chromatography, Affinity / Q000295:instrumentation*; D002850:Chromatography, Gel / Q000295:instrumentation*; D002851:Chromatography, High Pressure Liquid; D005110:Extracellular Space / Q000378:metabolism; D006801:Humans; D058225:Plaque, Amyloid / Q000378:metabolism*; D011108:Polymers; D011726:Pyridinium Compounds; D012685:Sepharose
chemical_list: D016564:Amyloid beta-Protein Precursor; D011108:Polymers; D011726:Pyridinium Compounds; C070192:2-fluoro-1-methylpyridinium fractogel; D012685:Sepharose; C049225:Superose 12