Brevundimonas lenta sp. nov., isolated from soil.
A Gram-negative, rod-shaped, Brevundimonas-like bacterial strain, DS-18(T), was isolated from soil in Dokdo, Korea, and its exact taxonomic position was investigated by using a polyphasic approach. Strain DS-18(T) grew optimally at pH 6.5-7.0 and 25 degrees C without NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-18(T) belonged to the genus Brevundimonas. Strain DS-18(T) contained Q-10 as the predominant ubiquinone and C(18 : 1)omega7c and C(16 : 0) as the major fatty acids. The DNA G+C content was 68.7 mol%. Strain DS-18(T) exhibited levels of 16S rRNA gene sequence similarity of 96.3-98.7 % to the type strains of Brevundimonas species and Mycoplana bullata. Mean DNA-DNA relatedness values between strain DS-18(T) and the type strains of phylogenetically related Brevundimonas species and M. bullata were in the range 15-32 %. Strain DS-18(T) differed from Brevundimonas species and M. bullata in several phenotypic characteristics. On the basis of phenotypic, phylogenetic and genetic data, strain DS-18(T) represents a novel species of the genus Brevundimonas, for which the name Brevundimonas lenta sp. nov. is proposed. The type strain is DS-18(T) (=KCTC 12871(T) =JCM 14602(T)).
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Kang
So-Jung
SJ
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Lee
Jung-Sook
JS
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Oh
Hyun Woo
HW
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Oh
Tae-Kwang
TK
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D042021:Brucellaceae / Q000235:genetics; D042063:Caulobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature; D014451:Ubiquinone / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D012965:Sodium Chloride