title: Biochemical characterization of cellulose-binding proteins (CBPA and CBPB) from the rumen cellulolytic bacterium Eubacterium cellulosolvens 5.
abstract: The cellulose-binding proteins, CBPA and CBPB, of rumen cellulolytic bacterium Eubacterium cellulosolvens 5 were biochemically characterized, and their properties were compared. Recombinant CBPA and CBPB were a typical 1,4-beta-endoglucanase. Both proteins bound to insoluble polysaccharides such as Avicel cellulose, acid swollen cellulose, lichenan, chitin, and oat spelt xylan. On the other hand, only recombinant CBPB bound to agarose and starch.
authors.affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Yoshimatsu
authors.forename: Atsushi
authors.initials: A
authors.lastname: Toyoda
authors.forename: Naoki
authors.initials: N
authors.lastname: Onizawa
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Hajime
authors.initials: H
authors.lastname: Minato
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002475:Cellobiose / Q000096:biosynthesis; D002482:Cellulose / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000378:metabolism; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005051:Eubacterium / Q000737:chemistry*; D005947:Glucose / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008970:Molecular Weight; D010957:Plasmids; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012417:Rumen / Q000382:microbiology*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D002475:Cellobiose; D002482:Cellulose; D043366:Cellulose 1,4-beta-Cellobiosidase; D005947:Glucose