title: In vitro anti-Helicobacter pylori activity of extracts of selected medicinal plants from North West Cameroon.
abstract: BACKGROUND
  Helicobacter pylori, a gram negative microaerophilic bacterium is a major etiological agent in duodenal, peptic and gastric ulcers. The growing problem of antibiotic resistance by the organism demands the search for novel compounds from plant based sources.
  
  
  OBJECTIVE
  The present study is aimed at evaluating the antimicrobial activity of some selected medicinal plants on clinical isolates of H. pylori circulating in Cameroon in a bid to identify potential sources of cheap starting materials for the synthesis of new drugs.
  
  
  METHODS
  Gastric biopsy samples were obtained from patients presenting with gastroduodenal complications. H. pylori was isolated from the specimens following standard microbiology procedures. The disk diffusion method was used to determine the susceptibility of 15 isolates to ten methanol plant extracts (Ageratum conyzoides, Scleria striatinux, Lycopodium cernua, Acanthus montanus, Eryngium foetidium, Aulutandria kamerunensis, Tapeinachilus ananassae, Euphorbia hirta, Emilia coccinea and Scleria verrucosa). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for the most active plant extracts were also determined by the agar dilution method. Results were analyzed statistically by the Fisher's exact test.
  
  
  RESULTS
  All the plants tested demonstrated antimicrobial activity with zone diameters of inhibition ranging from 0-30mm. Of these, A. conyzoides, S. striatinux and L. cernua showed very potent antibacterial activity on the isolates. The lowest MIC and MBC recorded were 0.032mg/mL and 0.098mg/mL respectively. However, the MIC of the extracts ranged from 0.032-1.0mg/mL for S. striatinux; 0.063-0.5mg/mL for L. cernua and 0.063-1.0mg/mL for A. conyzoides. The MBC of the extracts ranged from 0.098-15.0mg/mL for S. striatinux; 0.098-12.5mg/mL for A. conyzoides, and 0.195-12.5mg/mL for L. cernua. The extracts had a wide spectrum of activity. The three most potent extracts possessed significant (P<0.05) inhibitory activities.
  
  
  CONCLUSIONS
  The plant extracts may contain compounds with therapeutic activity.
authors.affiliation: Department of Biochemistry and Microbiology, University of Buea, Cameroon. ndip3@yahoo.com
authors.forename: Roland N
authors.initials: RN
authors.lastname: Ndip
authors.forename: Alertia E
authors.initials: AE
authors.lastname: Malange Tarkang
authors.forename: Susan M
authors.initials: SM
authors.lastname: Mbullah
authors.forename: Henry N
authors.initials: HN
authors.lastname: Luma
authors.forename: Agnes
authors.initials: A
authors.lastname: Malongue
authors.forename: Lucy M
authors.initials: LM
authors.lastname: Ndip
authors.forename: Kennedy
authors.initials: K
authors.lastname: Nyongbela
authors.forename: Clare
authors.initials: C
authors.lastname: Wirmum
authors.forename: Simon M N
authors.initials: SM
authors.lastname: Efange
mesh_terms: D002163:Cameroon; D016480:Helicobacter pylori / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal
chemical_list: D010936:Plant Extracts