Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog.
Two facultatively aerobic, heterotrophic bacteria capable of degrading pectin, xylan, laminarin and some other polysaccharides were obtained from the acidic Sphagnum peat bog Bakchar, in western Siberia, Russia, and were designated strains TPT18(T) and TPT56(T). Cells of these isolates are Gram-negative, non-motile, long rods that are covered by large capsules. On ageing, they transform into spherical L-forms. Strains TPT18(T) and TPT56(T) are acido- and psychrotolerant organisms capable of growth at pH 4.2-8.2 (with an optimum at pH 6.0-6.5) and at 2-33 degrees C (with an optimum at 20 degrees C). The major fatty acids are iso-C(15 : 0), anteiso-C(15 : 0), iso-C(17 : 0) 3-OH and summed feature 3 (iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c); the quinones are MK-7 and MK-6. Comparative 16S rRNA gene sequence analysis revealed that the novel strains share 97 % sequence similarity and belong to the family Sphingobacteriaceae; however, they are related only distantly to members of the genera Pedobacter (91.8-93.3 % similarity) and Sphingobacterium (89.6-91.2 % similarity). The DNA G+C content of strains TPT18(T) and TPT56(T) is 42.4 and 46.1 mol%, respectively. The low DNA-DNA hybridization value (42 %) and a number of phenotypic differences between strains TPT18(T) and TPT56(T) indicated that they represent two separate species. Since the two isolates are clearly distinct from all currently described members of the family Sphingobacteriaceae, we propose a novel genus, Mucilaginibacter gen. nov., containing two novel species, Mucilaginibacter gracilis sp. nov. and Mucilaginibacter paludis sp. nov. The type strains of Mucilaginibacter gracilis and Mucilaginibacter paludis are respectively TPT18(T) (=ATCC BAA-1391(T) =VKM B-2447(T)) and TPT56(T) (=ATCC BAA-1394(T) =VKM B-2446(T)).
Pankratov
Timofei A
TA
S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letya Octyabrya 7/2, Moscow 117312, Russia.
Tindall
Brian J
BJ
DSMZ - German Collection of Microorganisms and Cell Cultures, D-38124 Braunschweig, Germany.
Liesack
Werner
W
Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany.
Dedysh
Svetlana N
SN
S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letya Octyabrya 7/2, Moscow 117312, Russia.
D000332:Aerobiosis; D016667:Bacterial Capsules / Q000648:ultrastructure; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005936:Glucans; D006863:Hydrogen-Ion Concentration; D008124:Locomotion / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010368:Pectins / Q000378:metabolism*; D010802:Phylogeny; D011134:Polysaccharides / Q000378:metabolism*; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012800:Siberia; D012988:Soil Microbiology; D044003:Sphagnopsida; D013696:Temperature; D053833:Wetlands; D014990:Xylans / Q000378:metabolism*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D005936:Glucans; D011134:Polysaccharides; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014990:Xylans; D010368:Pectins; C008247:laminaran