title: Isolation, crystallization and preliminary crystallographic analysis of Salmonella typhimurium uridine phosphorylase crystallized with 2,2'-anhydrouridine.
abstract: Uridine phosphorylase (UPh; EC 2.4.2.3) is a member of the pyrimidine nucleoside phosphorylase family of enzymes which catalyzes the phosphorolytic cleavage of the C-N glycoside bond of uridine, with the formation of ribose 1-phosphate and uracil. This enzyme has been shown to be important in the activation and catabolism of fluoropyrimidines. Modulation of its enzymatic activity may affect the therapeutic efficacy of chemotherapeutic agents. The structural investigation of the bacterial uridine phosphorylases, both unliganded and complexed with substrate/product analogues and inhibitors, may help in understanding the catalytic mechanism of the phosphorolytic cleavage of uridine. Salmonella typhimurium uridine phosphorylase has been crystallized with 2,2'-anhydrouridine. X-ray diffraction data were collected to 2.15 A. Preliminary analysis of the diffraction data indicates that the crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 88.52, b = 123.98, c = 133.52 A. The solvent content is 45.51%, assuming the presence of one hexamer molecule per asymmetric unit.
authors.affiliation: A. V. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninskiy Prospect 59, 119333 Moscow, Russia.
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Timofeev
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Lashkov
authors.forename: Azat G
authors.initials: AG
authors.lastname: Gabdoulkhakov
authors.forename: Bogdan Ph
authors.initials: BP
authors.lastname: Pavlyuk
authors.forename: Galina S
authors.initials: GS
authors.lastname: Kachalova
authors.forename: Christian
authors.initials: C
authors.lastname: Betzel
authors.forename: Ekaterina Yu
authors.initials: EY
authors.lastname: Morgunova
authors.forename: Nadezhda E
authors.initials: NE
authors.lastname: Zhukhlistova
authors.forename: Al'bert M
authors.initials: AM
authors.lastname: Mikhailov
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D012486:Salmonella typhimurium / Q000201:enzymology*; D013379:Substrate Specificity; D014529:Uridine / Q000031:analogs & derivatives* / Q000737:chemistry; D014543:Uridine Phosphorylase / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: C007208:cyclouridine; D014543:Uridine Phosphorylase; D014529:Uridine