The role of CCL22/macrophage-derived chemokine in allergic rhinitis.
Dendritic cells (DCs) are considered to be the most powerful antigen-presenting cells (APCs). DCs are thought to be associated with Th1 or Th2 polarization and with polarization-induced disease such as atopic dermatitis, asthma and allergic rhinitis, but its mechanism is not well known. In this study, we analyzed the mRNA expression of DCs between birch pollen allergic rhinitis and healthy controls by using cDNA array. We found that the expressions of CCL22/macrophage-derived chemokine (MDC) differed significantly. We also revealed that CCL22/MDC production was higher in patients than in healthy donors. By chemotaxis assay, CCL22/MDC can enhance the migration of patient's T cells rather than those of healthy controls. Surface marker analysis of migrated cells revealed that the most of migrated cells expressed CCR4, which were considered to be Th2 cells. Furthermore, CD1a(+) CD83(+) cells located in the nasal mucosa expressed CCL22/MDC in vivo. To the best of our knowledge, this is the first report clearly indicating the role of CCL22/MDC in allergic rhinitis.
Yanai
Mitsuru
M
Department of Pathology, Asahikawa Medical College, Hokkaido, Japan. mitsuru@asahikawa-med.ac.jp
Sato
Keisuke
K
Aoki
Naoko
N
Takiyama
Yumi
Y
Oikawa
Kensuke
K
Kobayashi
Hiroya
H
Kimura
Shoji
S
Harabuchi
Yasuaki
Y
Tateno
Masatoshi
M
D015703:Antigens, CD / Q000096:biosynthesis; D029662:Betula / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D054422:Chemokine CCL22 / Q000276:immunology* / Q000378:metabolism; D018925:Chemokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D009297:Nasal Mucosa / Q000166:cytology / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D011058:Pollen / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D054398:Receptors, CCR4 / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology* / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism
D015703:Antigens, CD; C515354:CCR4 protein, human; D054422:Chemokine CCL22; D018925:Chemokines; D012333:RNA, Messenger; D054398:Receptors, CCR4