Surfactant protein-D and surfactant inhibit endotoxin-induced pulmonary inflammation.
BACKGROUND
Acute lung injury is a common cause of morbidity and mortality following pulmonary or systemic infections. Surfactant protein-D is a member of the collectin family of proteins, which play important roles in innate host defense of the lung. In this study, the effect of exogenous recombinant human SP-D (rhSP-D) on protection of the adult mouse lung from lipopolysaccharide (LPS)-induced and lipoteichoic acid (LTA)-induced injury was assessed.


METHODS
The effect of rhSP-D on LPS-induced and LTA-induced lung inflammation and injury was assessed with and without exogenous pulmonary surfactant in Sftpd+/+ and Sftpd-/- mice. A total of 204 mice (6 mice per group) were used for the present study.


RESULTS
Sftpd-/- mice were more susceptible to intratracheal LPS than were Sftpd+/+ mice. rhSP-D decreased neutrophilic infiltrates induced by LPS and LTA in the lungs of both Sftpd+/+ and Sftpd-/- mice. The addition of exogenous pulmonary surfactant to rhSP-D further decreased LPS-induced and LTA-induced pulmonary inflammation in Sftpd-/- and Sftpd+/+ mice.


CONCLUSIONS
Intratracheal rhSP-D inhibited inflammation induced by intratracheal LPS and LTA instillation in the lung. The antiinflammatory effects of rhSP-D were enhanced by the addition of pulmonary surfactant, providing a potential therapy for the treatment of lung inflammation.
Ikegami
Machiko
M
Cincinnati Children's Hospital, Division of Pulmonary Biology, 3333 Burnet Ave, University of Cincinnati, OH 45229-3039, USA. machiko.ikegami@cchmc.org
Scoville
Elizabeth A
EA
Grant
Shawn
S
Korfhagen
Thomas
T
Brondyk
William
W
Scheule
Ronald K
RK
Whitsett
Jeffrey A
JA
D000208:Acute Disease; D000704:Analysis of Variance; D000818:Animals; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000276:immunology*; D007322:Instillation, Drug; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology; D008168:Lung / Q000378:metabolism*; D051379:Mice; D037663:Pulmonary Surfactant-Associated Protein D / Q000276:immunology*; D011663:Pulmonary Surfactants / Q000276:immunology*; D013682:Teichoic Acids / Q000008:administration & dosage / Q000494:pharmacology
D008070:Lipopolysaccharides; D037663:Pulmonary Surfactant-Associated Protein D; D011663:Pulmonary Surfactants; D013682:Teichoic Acids; C009900:lipoteichoic acid