title: Design of an optical sensor for indirect determination of isoniazid.
abstract: The characterization of an optical sensor membrane is described for indirect determination of isoniazid. The sensing membrane was consisted of immobilized 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine (PDT) on a triacetylcellulose membrane. The procedure is based on the reaction of Fe(III) with isoniazid in the presence of PDT. Fe(III) is reduced by isoniazid to Fe(II) which forms a complex with PDT. The complex shows an absorption maximum at 558nm. By measuring the absorbance of the complex at this wavelength, isoniazid can be determined in the range of 0.62-6.15microgmL(-1). This method was applied to the determination of isoniazid in pharmaceutical formulation and enabled the determination of isoniazid in microgram quantities.
authors.affiliation: Department of Chemistry, Faculty of Sciences, Shiraz University, Shiraz 71454, Iran. safavi@chem.susc.ac.ir
authors.forename: Afsaneh
authors.initials: A
authors.lastname: Safavi
authors.forename: Mozhgan
authors.initials: M
authors.lastname: Bagheri
mesh_terms: D006863:Hydrogen-Ion Concentration; D007538:Isoniazid / Q000737:chemistry*; D013053:Spectrophotometry; D014227:Triazines / Q000737:chemistry
chemical_list: D014227:Triazines; C044359:3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine; D007538:Isoniazid