title: Low cytotoxicity effect of dendrosome as an efficient carrier for rotavirus VP2 gene transferring into a human lung cell line : dendrosome, as a novel intranasally gene porter.
abstract: The efficiency of dendrosome (a gene porter) was assessed in transferring recombinant human rotavirus VP2 cDNA into A549, a human lung cell line. After gene transferring, transmission electron microscopy showed core-like particles (CLPs) formation in the transfected cells both with dendrosome and lipofectamine porters. In addition, western blotting analysis showed that the expression of VP2 gene was almost equal in the dendrosome and lipofectamine-transfected cells. Also, the cytotoxicity studies revealed that dendrosome had a lower cytotoxicity than lipofectamine. Therefore, our study may introduce dendrosome as a possible carrier for gene transferring into the human lung cell line, especially, for intranasally administration of DNA vaccines.
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. pourasgari@ibb.ut.ac.ir
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Pourasgari
authors.forename: Shahin
authors.initials: S
authors.lastname: Ahmadian
authors.forename: Ali Hatef
authors.initials: AH
authors.lastname: Salmanian
authors.forename: Mohammad Nabi
authors.initials: MN
authors.lastname: Sarbolouki
authors.forename: Mohammad
authors.initials: M
authors.lastname: Massumi
mesh_terms: D036022:Capsid Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival; D004337:Drug Carriers / Q000737:chemistry*; D018014:Gene Transfer Techniques; D006801:Humans; D008055:Lipids / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D019444:Vaccines, DNA / Q000378:metabolism*; D014771:Virion / Q000648:ultrastructure
chemical_list: D036022:Capsid Proteins; D004337:Drug Carriers; D008055:Lipids; C086724:Lipofectamine; C442545:VP2 protein, Rotavirus; D019444:Vaccines, DNA