Fluorescent diethylcarbamazine analogues: sites of accumulation in Brugia malayi.
New fluorescein and rhodamine B-labeled antifilarial drug DEC analogues for use in drug localization studies with confocal microscopy have been prepared by a high-yield three-step synthesis. The resulting beta-amine-substituted DEC analogue has a single ethyl substituent which is beta-aminated to accommodate the fluorophore of either fluorescein isothiocyananate or rhodamine B. Confocal microscopy is used to show that the drug accumulates in the adult filarial worms in the pharynx, esophagus, and near the nerve ring of all adults, as well as in the uteri and vulva and the testes of the females and males.
Junnila
Amy
A
Institute of Parasitology and Department of Chemistry, McGill University, Montreal, Canada.
Bohle
D Scott
DS
Prichard
Roger
R
Perepichka
Inna
I
Spina
Carla
C
D000375:Aging / Q000187:drug effects / Q000502:physiology; D000818:Animals; D017178:Brugia malayi / Q000187:drug effects*; D004049:Diethylcarbamazine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology*; D015394:Molecular Structure
D005456:Fluorescent Dyes; D004049:Diethylcarbamazine