title: A simple improved method for protein extraction from human head hairs.
abstract: This study was conducted to establish a simple efficient method for extracting the protein from human head hair materials, which can be a useful tool for the protein analysis applicable to various types of human head hairs. The method developed saves extraction time and effort considerably. The method includes four steps: cutting the hair samples into small pieces 1-2 mm in length, washing them with distilled water, incubating the hair samples in a buffer solution at 50 degrees C for 24 hr, and finally filtering the incubated mixtures through three layers of nylon mesh. This method is reproducible and reliable. SDS-PAGE analysis of the hair protein extracted by this method shows a clear protein profile on the gel, which is frequently observed in other hair sources. Two smaller sizes of molecular weights are also detected with the SDS-PAGE analysis. Not commonly found in other hair sources, they seem to be other types of human hair proteins.
authors.affiliation: Department of Biotechnology, Dong-A University, Busan 604-714, South Korea.
authors.forename: Mi-Ok
authors.initials: MO
authors.lastname: Han
authors.forename: Jae-An
authors.initials: JA
authors.lastname: Chun
authors.forename: Woo-Hyup
authors.initials: WH
authors.lastname: Lee
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Lee
authors.forename: Chung-Han
authors.initials: CH
authors.lastname: Chung
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006197:Hair / Q000737:chemistry*; D006801:Humans; D011506:Proteins / Q000302:isolation & purification*; D015203:Reproducibility of Results
chemical_list: D011506:Proteins