Cloning of full genome sequence of hepatitis E virus of Shanghai swine isolate using RACE method.
Genotype 4 hepatitis E virus (HEV) was reportedly transmitted freely between humans and swine in eastern China. The full-length genomic sequence of Shanghai swine isolate (SH-SW-zs1) recovered from feces sample of a pig which was infected with HEV RNA positive swine serum was determined using RT-PCR and RACE (Rapid Amplification of cDNA Ends) methods. The full genome of the SH-SW-zs1 isolate was 7265 nucleotides in length and phylogenetic analysis indicated that this isolate belonged to genotype 4. Comparison of the 3' UTR sequence with the corresponding regions of other 38 HEV strains from different region revealed that the Shanghai swine isolate is 21-49 bp longer than the other stains.
Shen
Quan
Q
School of Agriculture and Biology, Shanghai JiaoTong University, 800 Dongchuan Road, Shanghai 200240, PR China. njnushenquan@yahoo.com.cn
Zhang
Wen
W
Cao
Xiangrong
X
Mou
Jing
J
Cui
Li
L
Hua
Xiuguo
X
D000818:Animals; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D016752:Hepatitis E virus / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010802:Phylogeny; D013552:Swine / Q000821:virology*