Red antenna states of photosystem I from Synechococcus sp. PCC 7002.
Absorption, fluorescence and single-molecule spectroscopy at low temperatures were used to elucidate spectral properties, heterogeneities and dynamics of the red-shifted chlorophyll a (Chla) molecules responsible for the fluorescence in photosystem I (PSI) from the cyanobacterium Synechoccocus sp. PCC 7002. The 77 K absorption spectrum indicates the presence of 2-3 red-shifted Chla's absorbing at about 708 nm. The fluorescence emission spectrum is dominated by a broad band at 714 nm. The emission spectra of single PSI complexes show zero-phonon lines (ZPLs) as well as a broad intensity distribution without ZPLs. The spectral region below 710 nm often shows ZPLs, they form a spectral band with a maximum at 698 nm (F698). The region above 710 nm is dominated by broad intensity distributions and the observation of ZPLs is less frequent. The broad distributions are due to the emission of the C708 Chla's and the emission from F698 stems from a Chla species absorbing at the blue side of P700. The properties of these two emissions show a close relation to those of the C708 and C719 pools observed in T. elongatus. Therefore an assignment of F698 and C708 to Chla-species with similarities to C708 and C719 in T. elongatus is proposed.
Brecht
Marc
M
Fachbereich Physik, Freie Universität Berlin, Arnimalle 14, 14195, Berlin, Germany.
Nieder
Jana B
JB
Studier
Hauke
H
Schlodder
Eberhard
E
Bittl
Robert
R
D045331:Photosystem I Protein Complex / Q000737:chemistry / Q000502:physiology*; D013050:Spectrometry, Fluorescence; D046940:Synechococcus / Q000737:chemistry / Q000502:physiology*
D045331:Photosystem I Protein Complex