CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription.
Chromatin remodeling and histone modification are essential for eukaryotic transcription regulation, but little is known about chromatin-modifying activities acting on RNA polymerase III (Pol III)-transcribed genes. The human U6 small nuclear RNA promoter, located 5' of the transcription start site, consists of a core region directing basal transcription and an activating region that recruits the transcription factors Oct-1 and Staf (ZNF143). Oct-1 activates transcription in part by helping recruit core binding factors, but nothing is known about the mechanisms of transcription activation by Staf. We show that Staf activates U6 transcription from a preassembled chromatin template in vitro and associates with several proteins linked to chromatin modification, among them chromodomain-helicase-DNA binding protein 8 (CHD8). CHD8 binds to histone H3 di- and trimethylated on lysine 4. It resides on the human U6 promoter as well as the mRNA IRF3 promoter in vivo and contributes to efficient transcription from both these promoters. Thus, Pol III transcription from type 3 promoters uses some of the same factors used for chromatin remodeling at Pol II promoters.
Yuan
Chih-Chi
CC
Stony Brook University, Graduate Program in Molecular and Cellular Biology, Stony Brook, New York 11794, USA.
Zhao
Xinyang
X
Florens
Laurence
L
Swanson
Selene K
SK
Washburn
Michael P
MP
Hernandez
Nouria
N
D000595:Amino Acid Sequence; D002843:Chromatin / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D050838:Interferon Regulatory Factor-3 / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008745:Methylation; D008969:Molecular Sequence Data; D011485:Protein Binding; D021381:Protein Transport; D012320:RNA Polymerase III; D012342:RNA, Small Nuclear / Q000235:genetics* / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation / Q000235:genetics
C523331:CHD8 protein, human; D002843:Chromatin; D004268:DNA-Binding Proteins; D006657:Histones; C494232:IRF3 protein, human; D050838:Interferon Regulatory Factor-3; D012342:RNA, Small Nuclear; D015534:Trans-Activators; D014157:Transcription Factors; C405483:U1 small nuclear RNA; C405486:U6 small nuclear RNA; C114253:ZNF143 protein, human; D012320:RNA Polymerase III; D008239:Lysine