title: The F-box protein CFB2 is required for cytokinesis of bloodstream-form Trypanosoma brucei.
abstract: F-box proteins serve as mediators in targeting bound target proteins for ubiquitination and destruction. We here describe the roles of two F-box proteins, CFB1 and CFB2, in the trypanosome cell cycle. Five almost identical copies of CFB1 are arranged in a direct tandem repeat on Trypanosoma brucei chromosome 1; immediately downstream is a single CFB2 gene. RNAi targeting CFB1 in bloodstream-form trypanosomes had a transient effect on growth and mitosis. Depletion of CFB2, in contrast, resulted in immediate growth arrest and rapid cell death. CFB2-depleted cells accumulated nuclei and kinetoplasts with the corresponding numbers of basal bodies and flagella. The CFB2 transcript was less abundant in procyclic-form trypanosomes, and RNAi against CFB2 in these forms had no effect on growth. These results suggest that CFB2 is required for bloodstream-form trypanosome cytokinesis.
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany.
authors.forename: Corinna
authors.initials: C
authors.lastname: Benz
authors.forename: Christine E
authors.initials: CE
authors.lastname: Clayton
mesh_terms: D000818:Animals; D016923:Cell Death; D048749:Cytokinesis / Q000235:genetics / Q000502:physiology*; D044783:F-Box Proteins / Q000235:genetics / Q000502:physiology*; D018628:Gene Dosage; D020868:Gene Silencing; D015800:Protozoan Proteins / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000502:physiology*
chemical_list: D044783:F-Box Proteins; D015800:Protozoan Proteins