title: Study of folliculogenesis in vivo in guinea pig.
abstract: Understanding normal folliculogenesis in guinea pigs is fundamental as a first step towards the development of a guinea pig follicle culture system. The aims of this study were (1) to characterise morphological changes during follicular development in vivo and (2) to describe the growth pattern of follicles. Cycling guinea pigs were infused with 5-bromo-2'-deoxyuridine for 1 or 2 weeks and sacrificed at time points ranging from 0 to 37 days after the infusion. The granulosa cell number in the largest cross-sections increased from 25.0+/-6.1 (mean+/-S.D.) in primary (type 2) to 192.0+/-65.9 in preantral (type 5) and 256.3+/-96.9 in antral (type 6) follicles. The oocyte diameter increased from 44.8+/-6.2 microm (type 2) to 72.8+/-9.1 microm (type 5) and 78.9+/-9.3 microm (type 6) and the follicle diameter from 67.9+/-10.1 microm (type 2) to 188.9+/-29.7 microm (type 5) and 231.0+/-56.1 microm (type 6). After a 1-week labelling period, about 71% of type 2 follicles had at least one labelled granulosa cell, as did 95% of type 3-4, and 100% of type 5 and 6. About 1 week was needed to achieve 95% mitotic activity in granulosa cells (GC) of type 5 and 6 follicles, while about 2 weeks was required to achieve 100% mitotic activity in GC of type 3-4 and more than 2 weeks for GC of type 2 follicles. These data provide some baselines for the examination of a guinea pig follicle culture system.
authors.affiliation: Free University Brussels, Center for Reproductive Medicine, Follicle Biology Laboratory, Laarbeeklaan 101, 1090 Brussels, Belgium. jcsadeu@uzbrussel.be
authors.forename: J C
authors.initials: JC
authors.lastname: Sadeu
authors.forename: I
authors.initials: I
authors.lastname: Adriaens
authors.forename: R
authors.initials: R
authors.lastname: Cortvrindt
authors.forename: J
authors.initials: J
authors.lastname: Smitz
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count; D005260:Female; D006107:Granulosa Cells / Q000166:cytology; D006168:Guinea Pigs; D007150:Immunohistochemistry; D023421:Models, Animal; D006080:Ovarian Follicle / Q000737:chemistry / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors
chemical_list: D001973:Bromodeoxyuridine