Maize streak virus-resistant transgenic maize: a first for Africa.
In this article, we report transgene-derived resistance in maize to the severe pathogen maize streak virus (MSV). The mutated MSV replication-associated protein gene that was used to transform maize showed stable expression to the fourth generation. Transgenic T2 and T3 plants displayed a significant delay in symptom development, a decrease in symptom severity and higher survival rates than non-transgenic plants after MSV challenge, as did a transgenic hybrid made by crossing T2 Hi-II with the widely grown, commercial, highly MSV-susceptible, white maize genotype WM3. To the best of our knowledge, this is the first maize to be developed with transgenic MSV resistance and the first all-African-produced genetically modified crop plant.
Shepherd
Dionne N
DN
Department of Molecular and Cell Biology, University of Cape Town, Private Bag, Rondebosch 7701, South Africa. dionne.shepherd@uct.ac.za
Mangwende
Tichaona
T
Martin
Darren P
DP
Bezuidenhout
Marion
M
Kloppers
Frederik J
FJ
Carolissen
Charlene H
CH
Monjane
Adérito L
AL
Rybicki
Edward P
EP
Thomson
Jennifer A
JA
D026762:Maize streak virus / Q000276:immunology*; D010935:Plant Diseases / Q000276:immunology; D030821:Plants, Genetically Modified / Q000276:immunology / Q000821:virology*; D019076:Transgenes; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D003313:Zea mays / Q000235:genetics / Q000276:immunology / Q000821:virology*
D017361:Viral Nonstructural Proteins