High frequency transformation of the industrial erythromycin-producing bacterium Saccharopolyspora erythraea.
The DNA transformation in the industrial erythromycin-producing Saccharopolyspora erythraea was investigated as standard protoplast transformation methods are ineffective. Intergeneric conjugal transfer of DNA from E. coli demonstrated transformation efficiencies from 0.05 x 10(-8) to 7.2 x 10(-8) exconjugants generated per recipient. Electroporation-mediated methodologies were also established. More than 10(5) transformants were acquired per mug DNA. The proposed protocol provides an alternative route for the introduction of DNA into industrial strains.
Wang
Yong
Y
State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, PR China.
Wang
YiGuang
Y
Zhang
Siliang
S
D019149:Bioreactors; D003227:Conjugation, Genetic; D018274:Electroporation; D004917:Erythromycin / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics; D016930:Saccharopolyspora / Q000235:genetics* / Q000378:metabolism*; D013997:Time Factors; D014170:Transformation, Genetic
D004917:Erythromycin