Pyrene-perylene as a FRET pair coupled to the N2'-functionality of 2'-amino-LNA.
Detection of nucleic acid hybridization via fluorescence resonance energy transfer (FRET) using pyren-1-ylmethyl and perylen-3-ylmethyl N2'-functionalized 2'-amino-LNA nucleosides incorporated into oligonucleotides exhibited a clear distance dependence of the FRET efficiency, ranging from below 10% when the fluorophores were approximately 40A apart to approximately 90% when the fluorophores were in close proximity.
Lindegaard
Dorthe
D
Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.
Madsen
Andreas S
AS
Astakhova
Irina V
IV
Malakhov
Andrei D
AD
Babu
B Ravindra
BR
Korshun
Vladimir A
VA
Wengel
Jesper
J
D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry*; D010569:Perylene / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011721:Pyrenes; D010569:Perylene; C030984:pyrene