Enhancement of europium emission band of europium tetracycline complex in the presence of cholesterol.
We report here the observation, for the first time, of the enhancement of Europium-Tetracycline complex emission in cholesterol solutions. This enhancement was initially observed with the addition of the enzyme cholesterol oxidase, which produces H(2)O(2), the agent driver of the Europium tetracycline complex, to the solution. However, it was found that the enzyme is not needed to enhance the luminescence. A calibration curve was determined, resulting in a simple method to measure the cholesterol quantity in a solution. This method shows that the complex can be used as a sensor to determine cholesterol in biological systems.
Silva
Flávia Rodrigues de Oliveira
FR
LSI, Escola Politécnica, Universidade de São Paulo, USP, São Paulo, SP, Brazil.
Samad
Ricardo Elgul
RE
Gomes
Laércio
L
Courrol
Lilia Coronato
LC
D002784:Cholesterol / Q000378:metabolism*; D002789:Cholesterol Oxidase / Q000378:metabolism; D005063:Europium / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D049449:Luminescence; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013752:Tetracycline / Q000737:chemistry* / Q000378:metabolism
D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D005063:Europium; D002784:Cholesterol; D002789:Cholesterol Oxidase; D013752:Tetracycline