title: Characterization of the PL-I-related SP2 protein from Xenopus.
abstract: The complete cDNA sequence of Xenopus laevis sperm specific proteins SP1 and SP2 has been determined. This information when taken together with N-terminal sequencing and mass spectroscopy data indicates that these two proteins share a product precursor relationship in which SP2 results from cleavage of a short N-terminal peptide of SP1. The secondary and tertiary structures of SP2 have been characterized using circular dichroism and three dimension structure prediction. These structural analyses have conclusively shown that SP1/SP2 proteins are related to proteins of the histone H1 family, particularly to vertebrate histone H1x. Hence, they can be considered bona fide members of the protamine-like- I (PL-I) group of sperm nuclear basic proteins (SNBPs) that have been described in other vertebrate and invertebrate groups. SP2 binds to nucleosomal DNA in a way that is very similar to that of histone H1. However, its interaction with circular DNA does not exhibit an enhanced preference for the supercoiled conformation, and it appears to be mainly driven by ionic interactions.
authors.affiliation: Department of Biochemistry and Microbiology, University of Victoria, Petch Building, 258, Victoria, B.C., Canada.
authors.forename: Lindsay J
authors.initials: LJ
authors.lastname: Frehlick
authors.forename: Adelina
authors.initials: A
authors.lastname: Prado
authors.forename: Alison
authors.initials: A
authors.lastname: Calestagne-Morelli
authors.forename: Juan
authors.initials: J
authors.lastname: Ausió
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D008954:Models, Biological; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D016329:Sp1 Transcription Factor / Q000737:chemistry / Q000235:genetics; D051704:Sp2 Transcription Factor / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D014981:Xenopus / Q000235:genetics*
chemical_list: D009687:Nuclear Proteins; D016329:Sp1 Transcription Factor; D051704:Sp2 Transcription Factor