Biodegradable films developed by electrospray deposition for sustained drug delivery.
The objective of present study is to develop biodegradable films with controllable thickness for sustained release applications using a combination of electrospray deposition techniques. The model anticancer drug-paclitaxel is encapsulated inside PLGA films. The morphology observed by atomic force microscopy and scanning electron microscopy reveals that the film has a flat surface together with a dense structure. X-ray photo-electron spectroscopy results show that some amount of paclitaxel is found on the surface layer of films. X-ray diffractometry (XRD) analysis suggests that paclitaxel is in an amorphous form in the polymer matrix even for up to 30% drug loading. Differential scanning calorimetry (DSC) study further proved that paclitaxel is in a solid solution state in polymer films. In vitro release profile indicates that sustained release of paclitaxel from the films is for more than 85 days, without the tri-phasic release profile typically for PLGA films. The phase contrast images clearly suggests a slight decrease in the number of C6 glioma cells as the paclitaxel loading within the polymeric films is increased. The results of MTT assay employed to quantify the cell viability correlates well with the observation from phase contrast microscopy.
Xie
Jingwei
J
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576.
Tan
Jun Chen
JC
Wang
Chi-Hwa
CH
D001932:Brain Neoplasms / Q000473:pathology; D002152:Calorimetry, Differential Scanning; D016503:Drug Delivery Systems; D005910:Glioma / Q000473:pathology; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D017239:Paclitaxel / Q000008:administration & dosage; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014961:X-Ray Diffraction
D017239:Paclitaxel