Altered dynamics of DNA bases adjacent to a mismatch: a cue for mismatch recognition by MutS.
The structural deviations as well as the alteration in the dynamics of DNA at mismatch sites are considered to have a crucial role in mismatch recognition followed by its repair utilizing mismatch repair family proteins. To compare the dynamics at a mismatch and a non-mismatch site, we incorporated 2-aminopurine, a fluorescent analogue of adenine next to a G.T mismatch, a C.C mismatch, or an unpaired T, and at several other non-mismatch positions. Rotational diffusion of 2-aminopurine at these locations, monitored by time-resolved fluorescence anisotropy, showed distinct differences in the dynamics. This alteration in the motional dynamics is largely confined to the normally matched base-pairs that are immediately adjacent to a mismatch/ unpaired base and could be used by MutS as a cue for mismatch-specific recognition. Interestingly, the enhanced dynamics associated with base-pairs adjacent to a mismatch are significantly restricted upon MutS binding, perhaps "resetting" the cues for downstream events that follow MutS binding. Recognition of such details of motional dynamics of DNA for the first time in the current study enabled us to propose a model that integrates the details of mismatch recognition by MutS as revealed by the high-resolution crystal structure with that of observed base dynamics, and unveils a minimal composite read-out involving the base mismatch and its adjacent normal base-pairs.
Nag
Nabanita
N
Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400005, India.
Rao
B J
BJ
Krishnamoorthy
G
G
D015075:2-Aminopurine / Q000737:chemistry; D000244:Adenosine Diphosphate / Q000378:metabolism; D020137:Base Pair Mismatch; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D053843:DNA Mismatch Repair; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D005453:Fluorescence; D015200:Fluoroimmunoassay; D051719:MutS DNA Mismatch-Binding Protein / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D015075:2-Aminopurine; D000244:Adenosine Diphosphate; D004247:DNA; D051719:MutS DNA Mismatch-Binding Protein