title: Augmented humoral and cellular immune responses induced by canine adenovirus type 1 DNA vaccine in BALB/c mice.
abstract: Infectious canine hepatitis (ICH) is caused by canine adenovirus type 1 (CAV-1), which severely harms infected animals. Vaccination provides an effective approach to preventing canine infectious diseases. With the objective of exploring a new vaccination strategy that may prevent or cure ICH, we constructed a DNA vaccine, pVAX1-CpG-Loop, and evaluated its immune efficacy. We found that vaccination of BALB/c mice with the DNA vaccine alone, or priming with DNA vaccine and boosting with the Loop protein, resulted in the following: (1) High-level specific antibody (IgG) against CAV-1 was induced; (2) T cell activation was elicited; and (3) neutralizing antibodies were detectable in immunized mice. Collectively, these data indicate that the availability of a DNA vaccine could prevent hepatitis contagiosa canis.
authors.affiliation: Department of Molecular Biology, Hebei Medical University, Shijiazhuang, People's Republic of China.
authors.forename: Jun-Xia
authors.initials: JX
authors.lastname: Wang
authors.forename: Long
authors.initials: L
authors.lastname: Zheng
authors.forename: Shu-Xia
authors.initials: SX
authors.lastname: Song
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Ying Fu
authors.initials: YF
authors.lastname: Liu
authors.forename: Shu-Han
authors.initials: SH
authors.lastname: Sun
mesh_terms: D006516:Adenoviruses, Canine / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004285:Dogs; D005260:Female; D006522:Hepatitis, Infectious Canine / Q000276:immunology*; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000097:blood; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D013601:T-Lymphocytes / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D022223:Vaccines, Subunit