title: Blood cell and vessel formation following transplantation of activin-treated explants in Xenopus.
abstract: Treatment of Xenopus blastula with activin converts undifferentiated presumptive ectoderm (animal cap) into mesoderm and endoderm in a dose-dependent manner. At low concentrations, activin induces ventral mesoderm such as blood cells. Here we show that activin-treated aggregates of animal cap cells prepared from undifferentiated presumptive ectoderm and transplanted into Xenopus embryos differentiated to form red blood cells and vascular endothelial cells. We compared gene expression profiles of the activin-treated with untreated aggregates of animal cap cells using microarray analysis. This revealed 838 clones including vascular-related genes that were expressed at levels at least 2-fold greater in the activin-treated aggregates than in the untreated controls. Of these, 356 were known Xenopus genes, 296 had homologues, and 186 were unknown genes. These findings identified novel vascular-related genes and provided insights into how the blood vessel system establishes in normal development.
authors.affiliation: Laboratory of Biochemistry, Hiroshima International University, Kure, Hiroshima, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nagamine
authors.forename: Miho
authors.initials: M
authors.lastname: Furue
authors.forename: Akimasa
authors.initials: A
authors.lastname: Fukui
authors.forename: Akira
authors.initials: A
authors.lastname: Matsuda
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Hori
authors.forename: Makoto
authors.initials: M
authors.lastname: Asashima
mesh_terms: D028341:Activins / Q000494:pharmacology*; D000818:Animals; D001773:Blood Cells / Q000187:drug effects / Q000502:physiology* / Q000637:transplantation*; D001808:Blood Vessels / Q000187:drug effects / Q000254:growth & development* / Q000637:transplantation*; D004625:Embryo, Nonmammalian; D042783:Endothelial Cells / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D016378:Tissue Transplantation; D014981:Xenopus
chemical_list: D012333:RNA, Messenger; D028341:Activins; D049452:Green Fluorescent Proteins; D012313:RNA