Direct selection of human genomic loci by microarray hybridization.
We applied high-density microarrays to the enrichment of specific sequences from the human genome for high-throughput sequencing. After capture of 6,726 approximately 500-base 'exon' segments, and of 'locus-specific' regions ranging in size from 200 kb to 5 Mb, followed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection targets. These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic regions.
Albert
Thomas J
TJ
NimbleGen Systems Inc., 1 Science Court, Madison, Wisconsin 53711, USA. talbert@nimblegen.com
Molla
Michael N
MN
Muzny
Donna M
DM
Nazareth
Lynne
L
Wheeler
David
D
Song
Xingzhi
X
Richmond
Todd A
TA
Middle
Chris M
CM
Rodesch
Matthew J
MJ
Packard
Charles J
CJ
Weinstock
George M
GM
Gibbs
Richard A
RA
D019313:BRCA1 Protein / Q000235:genetics; D045744:Cell Line, Tumor; D015723:Gene Library; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods
D019313:BRCA1 Protein; D009838:Oligodeoxyribonucleotides