title: Successful vaccination against Leishmania chagasi infection in BALB/c mice with freeze-thawed Leishmania antigen and Corynebacterium parvum.
abstract: This study evaluated the potential of a Leishmania antigen vaccine in protecting BALB/c mice against Leishmania chagasi. Mice received two subcutaneous doses of L. amazonensis vaccine with Corynebacterium parvum and subsequent boost was done without adjuvant. One week later, mice were challenged with L. chagasi. We observed that this vaccine caused a significant reduction in parasite load in liver and spleen and induced a high production of IFN-gamma and IL-4 by spleen cells from vaccinated mice in response to Leishmania antigen. Together, our data show that this vaccine is capable of inducing a Th1/Th2 response that is important to control parasite replication.
authors.affiliation: Laboratório de Imunoparasitologia, DECBI/NUPEB, Campus Universitário, Morro do Cruzeiro, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
authors.forename: Márcia de Carvalho
authors.initials: Mde C
authors.lastname: Vilela
authors.forename: Daniel Cláudio de Oliveira
authors.initials: DC
authors.lastname: Gomes
authors.forename: Eduardo de Almeida
authors.initials: Ede A
authors.lastname: Marques-da-Silva
authors.forename: Tiago Donatelli
authors.initials: TD
authors.lastname: Serafim
authors.forename: Luis Carlos Crocco
authors.initials: LC
authors.lastname: Afonso
authors.forename: Simone Aparecida
authors.initials: SA
authors.lastname: Rezende
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D007891:Leishmania / Q000276:immunology*; D007896:Leishmaniasis / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D054332:Leishmaniasis Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000627:therapeutic use; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D011425:Propionibacterium acnes / Q000276:immunology*; D013154:Spleen / Q000187:drug effects / Q000378:metabolism / Q000469:parasitology; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D054332:Leishmaniasis Vaccines; D015847:Interleukin-4; D007371:Interferon-gamma