[Metal accumulation and MTLP induction in the digestive glands of Perna viridis exposed to Cu].
Mussels have been proposed as appropriate biomonitors of marine pollution, especially for monitoring metallic pollution based on variations of metallothionein as biomarkers. Under 2 exposure levels (12.7 microg/L, 63.5 microg/L), Cu accumulation and metallothionein-like protein (MTLP) induction by mussel (Perna viridis) digestive glands were investigated and simulated into dynamic models in the present work, and the soluble and total Cu burden of digestive glands were also determined. Calculated mean Cu uptake rates by mussel target organ were 2.045 and 7.028 microg x (g x d)(-1) respectively, and the theoretical equilibrium kinetic BCFs of Cu were 2074 and 1619 correspondingly. And within the exposure duration, different changing trends of ratio of soluble Cu to total Cu in digestive glands were observed in the two groups. The MTLP level of control samples was (0.551 +/- 0.037) mg/g, and the counterparts are 0.407 - 0.699 mg/g, 0.826 - 0.942 mg/g respectively when mussels were exposed to 12.7 microg/L and 63.5 microg/L Cu solutions. Statistically significant MTLP induction (p < 0.001) was observed under higher exposure level. MTLP contents in digestive glands increased with the exposure Cu concentration and body accumulation of metal. There is a significantly negative exponential rise relationship (p < 0.000 1) between MTLP and Cu concentrations accumulated in the digestive glands of mussels.
Li
Chun-Di
CD
Key Laboratory of Marginal Sea Geology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China. Licd@scsio.ac.cn
Yan
Wen
W
Long
Ai-Min
AM
Ma
Fu-Jun
FJ
Chen
Shao-Yong
SY
D000818:Animals; D049872:Bivalvia / Q000187:drug effects / Q000378:metabolism*; D003300:Copper / Q000378:metabolism / Q000493:pharmacokinetics* / Q000633:toxicity; D004064:Digestive System / Q000187:drug effects / Q000378:metabolism; D004781:Environmental Exposure / Q000032:analysis; D008668:Metallothionein / Q000378:metabolism*; D014018:Tissue Distribution; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000493:pharmacokinetics* / Q000633:toxicity
D014874:Water Pollutants, Chemical; D003300:Copper; D008668:Metallothionein