title: Viability of Echinococcus granulosus protoscolices at different conditions.
abstract: The aim of this study was to examine the longest survival time of Echinococcus granulosus protoscolices stored at different humidities and constant temperatures from -10 to +40 degrees C. Sheep livers containing hydatid cysts obtained from slaughterhouses were taken to the laboratory within 3h and transferred into incubation cabinet previously set at -10, 0, +10, +20, +30 and +40 degrees C with different relative humidity (RH). Viability of protoscolices was assessed by 0.1% eosin staining. The longest survival times were 3 days at -10 degrees C (50% RH), 36 days at 0 degrees C (60% RH), 28 days at 10 degrees C (65% RH), 12 days at 20 degrees C (70% RH), 4 days at 30 degrees C (75% RH) and 3 days at 40 degrees C (80% RH).
authors.affiliation: Uludag University, Faculty of Veterinary Medicine, Parasitology Department, Gorukle Campus, 16059 Bursa, Turkey. ihsandiker@uludag.edu.tr
authors.forename: Ali Ihsan
authors.initials: AI
authors.lastname: Diker
authors.forename: Recep
authors.initials: R
authors.lastname: Tinar
authors.forename: Bayram
authors.initials: B
authors.lastname: Senlik
mesh_terms: D000818:Animals; D004444:Echinococcosis, Hepatic / Q000469:parasitology* / Q000662:veterinary; D048209:Echinococcus granulosus / Q000502:physiology*; D006813:Humidity; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology; D013048:Specimen Handling; D013696:Temperature; D013997:Time Factors