title: Microbacterium sediminicola sp. nov. and Microbacterium marinilacus sp. nov., isolated from marine environments.
abstract: Two novel Microbacterium species are described on the basis of phenotypic, chemotaxonomic and genotypic studies. The two strains, designated YM10-847(T) and YM11-607(T), were isolated from river sediment and unidentified hydroid, respectively, of a marine lake. The strains were Gram-positive, catalase-positive bacteria with l-ornithine as the diagnostic diamino acid of the peptidoglycan. The acyl type of the peptidoglycan was N-glycolyl. The major menaquinones were MK-10 and MK-11 for YM10-847(T), and MK-11 and MK-12 for YM11-607(T). Mycolic acids were not detected. The DNA G+C content of strains YM10-847(T) and YM11-607(T) was 67.8 and 71.6 mol%, respectively. Comparative 16S rRNA gene sequence analysis revealed that the two strains belong to the genus Microbacterium. DNA-DNA relatedness data showed that YM10-847(T) and YM11-607(T) are two novel species of this genus. On the basis of these results, strains YM10-847(T) and YM11-607(T) represent two novel species of the genus Microbacterium, for which the names Microbacterium sediminicola sp. nov. and Microbacterium marinilacus sp. nov. are proposed. The type strains are YM10-847(T) (=MBIC08264(T)=DSM 18905(T)) and YM11-607(T) (=MBIC07778(T)=DSM 18904(T)), respectively.
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kageyama
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Matsuo
authors.affiliation: Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kasai
authors.affiliation: Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Shizuri
authors.affiliation: The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan.|Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ōmura
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D007564:Japan; D008969:Molecular Sequence Data; D009171:Mycolic Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D009952:Ornithine / Q000032:analysis; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009171:Mycolic Acids; D010457:Peptidoglycan; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D009952:Ornithine; D002374:Catalase