Comparative studies on expression of alpha-smooth muscle actin in hepatic stellate cells in chronic hepatitis B and C.
OBJECTIVE
Chronic hepatitis B and hepatitis C are common causes of liver fibrosis and cirrhosis. We performed a comparative study on the expression of alpha-smooth muscle actin (alpha-SMA) in hepatic stellate cells (HSCs), a marker of HSC activation, in patients with chronic hepatitis B and hepatitis C.


METHODS
Using immunohistochemistry and a semi-quantitative scoring method, the expression of alpha-SMA in HSCs was studied in patients with chronic hepatitis B (n = 50) and hepatitis C (n = 50). The HSC activation index was correlated with age, sex, AST, ALT, viral genotype, viral titers, degrees of hepatic steatosis, necroinflammatory grades and fibrotic stages.


RESULTS
The HSC activation index correlated significantly (P < 0.05) with necroinflmmatory grades and fibrotic stages in chronic hepatitis B or hepatitis C. Besides, the HSC activation index also correlated significantly (P < 0.05) with hepatic steatosis and marginally significantly (P = 0.08) with serum viral titers in chronic hepatitis C. There was no significant difference in biochemical and histological activities between patients with hepatitis B and hepatitis C, but the latter had a significantly higher HSC activation index than the former. Multiple regression analysis in all 100 patients showed that the HSC activation index correlated significantly (P < 0.05) with necroinflammatory grades, fibrotic stages and hepatitis C (versus hepatitis B).


CONCLUSIONS
In chronic viral hepatitis, the HSC activation index correlated significantly and independently with necroinflammation and fibrosis. Additionally, the HSC activation index was significantly higher in patients with chronic hepatitis C. These data may be compatible with the postulation that hepatitis C virus can directly activate HSCs.
Chu
Chia-Ming
CM
Liver Research Unit, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taipei, Taiwan. chiamingchu@yahoo.com.tw
Shyu
Wei-Chue
WC
Liaw
Yun-Fan
YF
D000199:Actins / Q000378:metabolism*; D000328:Adult; D005260:Female; D019694:Hepatitis B, Chronic / Q000378:metabolism*; D019698:Hepatitis C, Chronic / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology*; D008103:Liver Cirrhosis / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009130:Muscle, Smooth / Q000378:metabolism*; D011863:Radioimmunoassay; D012044:Regression Analysis; D018709:Statistics, Nonparametric
D000199:Actins