title: Microtubule motors regulate ISOC activation necessary to increase endothelial cell permeability.
abstract: Calcium store depletion activates multiple ion channels, including calcium-selective and nonselective channels. Endothelial cells express TRPC1 and TRPC4 proteins that contribute to a calcium-selective store-operated current, I(SOC). Whereas thapsigargin activates the I(SOC) in pulmonary artery endothelial cells (PAECs), it does not activate I(SOC) in pulmonary microvascular endothelial cells (PMVECs), despite inducing a significant rise in global cytosolic calcium. Endoplasmic reticulum exhibits retrograde distribution in PMVECs when compared with PAECs. We therefore sought to determine whether endoplasmic reticulum-to-plasma membrane coupling represents an important determinant of I(SOC) activation in PAECs and PMVECs. Endoplasmic reticulum organization is controlled by microtubules, because nocodozole induced microtubule disassembly and caused retrograde endoplasmic reticulum collapse in PMVECs. In PMVECs, rolipram treatment produced anterograde endoplasmic reticulum distribution and revealed a thapsigargin-activated I(SOC) that was abolished by nocodozole and taxol. Microtubule motors control organelle distribution along microtubule tracks, with the dynein motor causing retrograde movement and the kinesin motor causing anterograde movement. Dynamitin expression reduces dynein motor function inducing anterograde endoplasmic reticulum transport, which allows for direct activation of I(SOC) by thapsigargin in PMVECs. In contrast, expression of dominant negative kinesin light chain reduces kinesin motor function and induces retrograde endoplasmic reticulum transport; dominant negative kinesin light chain expression prevented the direct activation of I(SOC) by thapsigargin in PAECs. I(SOC) activation is an important step leading to disruption of cell-cell adhesion and increased macromolecular permeability. Thus, microtubule motor function plays an essential role in activating cytosolic calcium transitions through the membrane I(SOC) channel leading to endothelial barrier disruption.
authors.affiliation: Department of Molecular and Cellular Pharmacology, College of Medicine, University of South Alabama, Mobile, Alabama 36688, USA. swu@jaguar1.usouthal.edu
authors.forename: Songwei
authors.initials: S
authors.lastname: Wu
authors.forename: Hairu
authors.initials: H
authors.lastname: Chen
authors.forename: Mikhail F
authors.initials: MF
authors.lastname: Alexeyev
authors.forename: Judy A C
authors.initials: JA
authors.lastname: King
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Moore
authors.forename: Troy
authors.initials: T
authors.lastname: Stevens
authors.forename: Ronald D
authors.initials: RD
authors.lastname: Balczon
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D000072159:Dynactin Complex; D004594:Electrophysiology; D004721:Endoplasmic Reticulum / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D016547:Kinesins / Q000737:chemistry; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis; D008870:Microtubules / Q000378:metabolism*; D008954:Models, Biological; D015739:Nocodazole / Q000494:pharmacology; D010539:Permeability; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D051381:Rats; D020889:Rolipram / Q000494:pharmacology; D050052:TRPC Cation Channels / Q000378:metabolism; D019284:Thapsigargin / Q000378:metabolism; D050257:Tubulin Modulators / Q000494:pharmacology
chemical_list: C000606361:DCTN2 protein, human; D000072159:Dynactin Complex; D008869:Microtubule-Associated Proteins; D010726:Phosphodiesterase Inhibitors; D050052:TRPC Cation Channels; D050257:Tubulin Modulators; D019284:Thapsigargin; D016547:Kinesins; D020889:Rolipram; D015739:Nocodazole