Dp71, utrophin and beta-dystroglycan expression and distribution in PC12/L6 cell cocultures.
Function of dystrophin Dp71 isoforms is unknown but seems related to neurite outgrowth and synapse formation. To evaluate Dp71 role in myoneural synapses, we established a coculture model using PC12 cells and L6 myotubes and analyzed expression and localization of Dp71 and related proteins, utrophin and beta-dystroglycan, in PC12 cells. Confocal microscopy showed Dp71d isoform in PC12 nuclei, golgi-complex-like and endoplasmic reticulum-like structures, whereas Dp71ab concentrates at neurite tips and cytoplasm, colocalizing with beta-dystroglycan, utrophin, synaptophysin and acetylcholine receptors. Evidences suggest that Dp71ab isoform, unlike Dp71d, may take part in neurite-related processes. This is the first work on Dp and members of Dp-associated protein complex roles in a cell-line based coculturing system, which may be useful in determining Dp71 isoforms associations.
Ilarraza-Lomeli
Ramses
R
Department of Genetics and Molecular Biology, Centre for Research and Advanced Studies (CINVESTAV-IPN), San Pedro Zacatenco, Mexico City, Mexico.
Cisneros-Vega
Bulmaro
B
Cervantes-Gomez
Maria de Lourdes
Mde L
Mornet
Dominique
D
Montañez
Cecilia
C
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D018920:Coculture Techniques; D049030:Dystroglycans / Q000235:genetics / Q000378:metabolism*; D016189:Dystrophin / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D018485:Muscle Fibers, Skeletal / Q000378:metabolism* / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000196:embryology / Q000294:innervation / Q000378:metabolism; D016501:Neurites / Q000378:metabolism / Q000648:ultrastructure; D009469:Neuromuscular Junction / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure; D016716:PC12 Cells; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011950:Receptors, Cholinergic / Q000378:metabolism; D013570:Synaptic Membranes / Q000378:metabolism / Q000648:ultrastructure; D016708:Synaptophysin / Q000378:metabolism; D049411:Utrophin / Q000235:genetics / Q000378:metabolism*
D016189:Dystrophin; D020033:Protein Isoforms; D011950:Receptors, Cholinergic; D016708:Synaptophysin; D049411:Utrophin; C085339:apo-dystrophin 1; D049030:Dystroglycans