title: A protective and broadly cross-neutralizing epitope of human papillomavirus L2.
abstract: We generated a monoclonal antibody, RG-1, that binds to highly conserved L2 residues 17 to 36 and neutralizes human papillomavirus 16 (HPV16) and HPV18. Passive immunotherapy with RG-1 was protective in mice. Antiserum to the HPV16 L2 peptide comprising residues 17 to 36 (peptide 17-36) neutralized pseudoviruses HPV5, HPV6, HPV16, HPV 18, HPV31, HPV 45, HPV 52, HPV 58, bovine papillomavirus 1, and HPV11 native virions. Depletion of HPV16 L2 peptide 17-36-reactive antibodies from cross-neutralizing rabbit and human L2-specific sera abolished cross-neutralization and drastically reduced neutralization of the cognate type. This cross-neutralization of diverse HPVs associated with cervical cancer, genital warts, and epidermodysplasia verruciformis suggests the possibility of a broadly protective, peptide-based vaccine.
authors.affiliation: Department of Pathology, The Johns Hopkins University, Baltimore, Maryland 21231, USA.
authors.forename: Ratish
authors.initials: R
authors.lastname: Gambhira
authors.forename: Balasubramanyam
authors.initials: B
authors.lastname: Karanam
authors.forename: Subhashini
authors.initials: S
authors.lastname: Jagu
authors.forename: Jeffrey N
authors.initials: JN
authors.lastname: Roberts
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Buck
authors.forename: Ioannis
authors.initials: I
authors.lastname: Bossis
authors.forename: Hannah
authors.initials: H
authors.lastname: Alphs
authors.forename: Timothy
authors.initials: T
authors.lastname: Culp
authors.forename: Neil D
authors.initials: ND
authors.lastname: Christensen
authors.forename: Richard B S
authors.initials: RB
authors.lastname: Roden
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology*; D000914:Antibodies, Viral / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology*; D003429:Cross Reactions; D052162:Human papillomavirus 16 / Q000276:immunology / Q000472:pathogenicity; D006801:Humans; D007116:Immunization, Passive; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D027383:Papillomaviridae / Q000145:classification* / Q000276:immunology*; D030361:Papillomavirus Infections / Q000517:prevention & control
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins; C063316:L2 protein, Human papillomavirus type 16; C099170:L2 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral