Relative expression of 1,25-dihydroxyvitamin D3 receptor, vitamin D 1 alpha-hydroxylase, vitamin D 24-hydroxylase, and vitamin D 25-hydroxylase in endometriosis and gynecologic cancers.
The authors demonstrate expression of the vitamin D receptor (VDR) and its hydroxylases in the endometrium and ovaries of women with and without endometriosis and endometrial or ovarian cancer. Immunohistochemistry showed strong staining of the VDR in endometriosis and endometrial cancer, with the most intense staining in epithelial cells. The VDR mRNA was significantly increased in patients with endometrial and ovarian cancer compared to the control group. There was a significantly higher 1 alpha-hydroxylase expression in the endometrium of patients with endometriosis compared to healthy controls. The observed differences in VDR and 1 alpha -hydroxylase mRNA levels were maintained at the protein level. The authors found no differences in 25-OH vitamin D levels between the serum of patients with endometriosis (25.7 +/- 2.1 ng/mL, n = 46) and healthy controls (22.6 +/- 2.0 ng/mL, n = 33, P = .31). They hypothesize that vitamin D might influence the local activity of immune cells and cytokines thought to play important pathogenic roles in the development and maintenance of endometriosis.
Agic
Admir
A
Department of Gynecology and Obstetrics, University of Schleswig-Holstein, Lübeck, Germany.
Xu
Hong
H
Altgassen
Christopher
C
Noack
Frank
F
Wolfler
Monika M
MM
Diedrich
Klaus
K
Friedrich
Michael
M
Taylor
Robert N
RN
Hornung
Daniela
D
D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D053493:Cholestanetriol 26-Monooxygenase / Q000096:biosynthesis* / Q000235:genetics; D016889:Endometrial Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D004715:Endometriosis / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D006801:Humans; D010051:Ovarian Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D018167:Receptors, Calcitriol / Q000096:biosynthesis* / Q000235:genetics; D013250:Steroid Hydroxylases / Q000096:biosynthesis* / Q000235:genetics; D065668:Vitamin D3 24-Hydroxylase
D018167:Receptors, Calcitriol; D013250:Steroid Hydroxylases; C406322:vitamin D 1-alpha hydroxylase; D053493:Cholestanetriol 26-Monooxygenase; D065668:Vitamin D3 24-Hydroxylase; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase