title: Escherichia coli HU protein has a role in the repair of abasic sites in DNA.
abstract: HU is one of the most abundant DNA binding proteins in Escherichia coli. We find that it binds strongly to DNA containing an abasic (AP) site or tetrahydrofuran (THF) (apparent K(d) approximately 50 nM). It also possesses an AP lyase activity that cleaves at a deoxyribose but not at a THF residue. The binding and cleavage of an AP site was observed only with the HUalphabeta heterodimer. Site-specific mutations at K3 and R61 residues led to a change in substrate binding and cleavage. Both K3A(alpha)K3A(beta) and R61A(alpha)R61A(beta) mutant HU showed significant reduction in binding to DNA containing AP site; however, only R61A(alpha)R61A(beta) mutant protein exhibited significant loss in AP lyase activity. Both K3A(alpha)K3A(beta) and R61K(alpha)R61K(beta) showed slight reduction in AP lyase activities. The function of HU protein as an AP lyase was confirmed by the ability of hupA or hupB mutations to further reduce the viability of an E. coli dut(Ts) xth mutant, which generates lethal AP sites at 37 degrees C; the hupA and hupB derivatives, respectively, had a 6-fold and a 150-fold lower survival at 37 degrees C than did the parental strain. These data suggest, therefore, that HU protein plays a significant role in the repair of AP sites in E. coli.
authors.affiliation: Department of Radiation Oncology, Emory University School of Medicine, Clifton Road NE, Atlanta, GA 30322, USA. ykow@emory.edu
authors.forename: Yoke W
authors.initials: YW
authors.lastname: Kow
authors.forename: Barry
authors.initials: B
authors.lastname: Imhoff
authors.forename: Bernard
authors.initials: B
authors.lastname: Weiss
authors.forename: David C I
authors.initials: DC
authors.lastname: Hung
authors.forename: Antreas A
authors.initials: AA
authors.lastname: Hindoyan
authors.forename: Randall M
authors.initials: RM
authors.lastname: Story
authors.forename: Steven D
authors.initials: SD
authors.lastname: Goodman
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004260:DNA Repair; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D043223:Deoxyribonuclease IV (Phage T4-Induced) / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009154:Mutation; D013696:Temperature
chemical_list: D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C116822:hns protein, E coli; D004247:DNA; D043223:Deoxyribonuclease IV (Phage T4-Induced); D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase