title: Does benign prostatic hyperplasia originate from the peripheral zone of the prostate? A preliminary study.
abstract: OBJECTIVE
  To compare the histological characteristics, cell proliferation, apoptosis and biological features in benign prostatic hyperplasia (BPH) in the peripheral (PZ) and transition zone (TZ) of the prostate.
  
  
  METHODS
  Tissue from BPH in TZ and PZ was obtained from 68 patients undergoing transrectal ultrasonography-guided biopsy and used for both morphometric analysis and immunohistochemical studies. The epithelial, stromal and luminal composition of the tissue was determined using a computer-assisted method for quantitative morphometric analysis. Apoptosis was detected as the apoptotic index (AI) using the TdT dUTP nick-end labelling assay. Cell proliferation was determined as the proliferation index (PI) using Ki-67 immunostaining. The expression of epidermal growth factor receptor (EGFR), transforming growth factor beta1 (TGFbeta1), androgen receptor (AR) and bcl-2 were assessed immunohistochemically.
  
  
  RESULTS
  There was no difference in the stroma/epithelium ratio between PZ and TZ hyperplastic nodules (P > 0.05). The mean AI in epithelium was almost identical to the corresponding PI. In stroma, no apoptotic cells were detectable. There was a significantly higher PI and AI in the glandular epithelial cells in PZ hyperplastic than in TZ hyperplastic nodules, but no difference in PI of the stromal cells between PZ and TZ hyperplastic nodules. There was significantly higher expression of TGFbeta1 and lower expression of EGFR and bcl-2 in PZ than TZ hyperplastic nodules (P < 0.05). There was no difference in AR expression between PZ and TZ hyperplastic nodules (P > 0.05).
  
  
  CONCLUSIONS
  These results indicate that some hyperplastic nodules in PZ might originate from the PZ, and the formation of these nodules might be modulated in a different way from that in the TZ.
authors.affiliation: Department of Ultrasound, Chinese People's Liberation Army General Hospital, Beijing, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
authors.forename: Jing Chun
authors.initials: JC
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Lihai
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhili
authors.initials: Z
authors.lastname: Wang
authors.forename: Junlai
authors.initials: J
authors.lastname: Li
authors.forename: Yukun
authors.initials: Y
authors.lastname: Luo
authors.forename: Jianhong
authors.initials: J
authors.lastname: Xu
authors.forename: Huaiyin
authors.initials: H
authors.lastname: Shi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D049109:Cell Proliferation; D066246:ErbB Receptors / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D011470:Prostatic Hyperplasia / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011944:Receptors, Androgen / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014463:Ultrasonography
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D011944:Receptors, Androgen; D053773:Transforming Growth Factor beta1; D066246:ErbB Receptors