title: Expression of TNF-alpha protein in omental and subcutaneous adipose tissue in obesity.
abstract: BACKGROUND
  During the last several years the role of adipose tissue in contributing to obesity-associated cardiovascular and metabolic risk has gained much attention.
  
  
  OBJECTIVE
  To examine the expression of TNF-alpha protein in omental and subcutaneous adipose tissue in correlation with plasma PAI-1 and other clinical parameters in obesity subjects.
  
  
  METHODS
  Paired biopsies of omental and subcutaneous fat were collected during surgery in 32 obesity subjects. The expression of TNF-alpha protein in omental and subcutaneous fat was quantified by using Western blot method, and correlations with plasma PAI-1, homeostasis model assessment insulin resistance (HOMA-IR) and lipid were investigated.
  
  
  RESULTS
  TNF-alpha protein expression was higher in omental than in subcutaneous adipose tissue (P<0.01). Significant positive linear correlations were found between TNF-alpha protein in omental adipose tissue and plasma PAI-1 in obesity subjects. TNF-alpha protein in omental fat was positively associated with HOMA-IR, triglycerides and negatively with HDL-cholesterol.
  
  
  CONCLUSIONS
  TNF-alpha expression in omental adipose tissue could play a key role in contributing to cardiovascular risk in central obesity subjects.
authors.affiliation: Department of Endocrinology, First Affiliated Hospital of China Medical University, No. 155, Nanjing North Street, Heping District, Shenyang, Liaoning 110001, China.
authors.forename: Yan Li
authors.initials: YL
authors.lastname: Cao
authors.forename: Cui Zhu
authors.initials: CZ
authors.lastname: Hu
authors.forename: Xin
authors.initials: X
authors.lastname: Meng
authors.forename: Di Fei
authors.initials: DF
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000273:Adipose Tissue / Q000503:physiopathology; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000453:epidemiology; D005260:Female; D015391:Gastroplasty; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000150:complications / Q000503:physiopathology* / Q000601:surgery; D009852:Omentum; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D012867:Skin; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D017395:Plasminogen Activator Inhibitor 1; D014409:Tumor Necrosis Factor-alpha