B- and T-cell-specific inactivation of thioredoxin reductase 2 does not impair lymphocyte development and maintenance.
Thioredoxin reductases (Txnrds) are a group of selenoenzymes participating in cellular redox regulation. Three Txnrd isoforms are known, each of which exhibits distinct cellular localisation and tissue-specific expression pattern. Txnrd1 is found in the cytoplasm, expression of Txnrd2 is restricted to mitochondria and Txnrd3 shows testis-specific expression. Recently, it was shown that Txnrd2 strongly affects the development of blood cells, since mouse embryos deficient for Txnrd2 are severely anaemic, show increased apoptosis in foetal liver and possess haematopoietic liver stem cells of reduced capacity to proliferate in vitro. However, because Txnrd2-deficient mice die at embryonic day 13.5, it was not known how this enzyme affects blood cell function in the adult animal. In the present study we show that conditional Txnrd2 knockouts generated using CD4- and CD19Cre transgenic mice lack Txnrd2 expression in CD4-- and CD19-positive T- and B-lymphocytes, respectively. However, the development and differentiation of both cell types in thymus and bone marrow was not significantly impaired. In addition, B-cell proliferation and activation in response to CD40 and IL-4 was unaltered in Txnrd2-deficient B-cells.
Geisberger
Roland
R
Department of Comparative Medicine, GSF Research Centre for Environment and Health, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany. roland@mrc-lmb.cam.ac.uk
Kiermayer
Claudia
C
Hömig
Cornelia
C
Conrad
Marcus
M
Schmidt
Jörg
J
Zimber-Strobl
Ursula
U
Brielmeier
Markus
M
D000818:Animals; D001402:B-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D002454:Cell Differentiation; D005260:Female; D005434:Flow Cytometry; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D054448:Precursor Cells, B-Lymphoid / Q000201:enzymology / Q000276:immunology; D054504:Precursor Cells, T-Lymphoid / Q000201:enzymology / Q000276:immunology; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D054482:Thioredoxin Reductase 2 / Q000235:genetics / Q000502:physiology*
D054482:Thioredoxin Reductase 2; C516128:Txnrd2 protein, mouse