title: Proteomic analysis of peritoneal dialysate fluid in patients with different types of peritoneal membranes.
abstract: Efficacy of peritoneal dialysis is determined by solute transport through peritoneal membranes. With the use of the peritoneal equilibration test (PET), peritoneal membranes can be classified as high (H), high average (HA), low average (LA), and low (L) transporters, based on the removal or transport rate of solutes, which are small molecules. Whether there is any difference in macromolecules (i.e., proteins) removed by different types of peritoneal membranes remains unclear. We performed a gel-based differential proteomics study of peritoneal dialysate effluents (PDE) obtained from chronic peritoneal dialysis (CPD) patients with H, HA, LA, and L transport rates (n=5 for each group; total n=20). Quantitative analysis and ANOVA with Tukey's posthoc multiple comparisons revealed five proteins whose abundance in PDE significantly differed among groups. These proteins were successfully identified by matrix-assisted laser desorption ionization quadrupole time-of-flight (MALDI-Q-TOF) mass spectrometry (MS) and tandem mass spectrometry (MS/MS) analyses, including serum albumin in a complex with myristic acid and triiodobenzoic acid, alpha1-antitrypsin, complement component C4A, immunoglobulin kappa light chain, and apolipoprotein A-I. The differences among groups in PDE levels of C4A and immunoglobulin kappa were clearly confirmed in a validation set of the other 24 patients (n=6 for each group) using ELISA. These data may lead to better understanding of the physiology of peritoneal membrane transport in CPD patients. Extending the study to a larger number of patients with subgroup analyses may yield additional information of the peritoneal dialysate proteins in association with dialysis adequacy, residual renal function, nutritional status, and risk of peritoneal infection.
authors.affiliation: Division of Nephrology, Department of Internal Medicine, Medical Molecular Biology Unit, Office for Research and Development, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Suchai
authors.initials: S
authors.lastname: Sritippayawan
authors.forename: Wararat
authors.initials: W
authors.lastname: Chiangjong
authors.forename: Theptida
authors.initials: T
authors.lastname: Semangoen
authors.forename: Nipa
authors.initials: N
authors.lastname: Aiyasanon
authors.forename: Parnthip
authors.initials: P
authors.lastname: Jaetanawanitch
authors.forename: Supachok
authors.initials: S
authors.lastname: Sinchaikul
authors.forename: Shui-Tein
authors.initials: ST
authors.lastname: Chen
authors.forename: Somkiat
authors.initials: S
authors.lastname: Vasuvattakul
authors.forename: Visith
authors.initials: V
authors.lastname: Thongboonkerd
mesh_terms: D016632:Apolipoprotein A-I / Q000737:chemistry; D001202:Ascitic Fluid / Q000378:metabolism; D001692:Biological Transport; D015934:Complement C4a / Q000737:chemistry; D015314:Dialysis Solutions / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D010530:Peritoneal Dialysis / Q000295:instrumentation*; D010537:Peritoneum / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D051437:Renal Insufficiency / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D000515:alpha 1-Antitrypsin / Q000737:chemistry
chemical_list: D016632:Apolipoprotein A-I; D015314:Dialysis Solutions; D007136:Immunoglobulins; D000515:alpha 1-Antitrypsin; D015934:Complement C4a