title: Oxidised cholesterol is more hypercholesterolaemic and atherogenic than non-oxidised cholesterol in hamsters.
abstract: The present study was to test the relative hypercholesterolaemic and atherogenic potency of oxidised cholesterol (OxC) and non-oxidised cholesterol in hamsters. An OxC mixture, prepared by heating pure cholesterol (100 g) at 160 degrees C in air for 72 h, contained 78 % cholesterol and 22 % OxC. Fifty Golden Syrian hamsters were randomly divided into five groups of ten animals and fed the control diet, a 0.05 % cholesterol diet (C-0.05), a 0.10 % cholesterol diet (C-0.1), a 0.05 % OxC mixture diet (OxC-0.05) or a 0.10 % OxC mixture diet (OxC-0.1), respectively. The OxC-0.05 and OxC-0.1 groups were more hypercholesterolaemic and had serum total cholesterol 22 and 12 % higher than the corresponding C-0.05 and C-0.1 hamsters (P < 0.05). The OxC-0.1 group demonstrated greater deposition of cholesterol and had a larger area of atherosclerotic plaque in the aorta than the corresponding C-0.1 hamsters (P < 0.05). Similarly, the aorta in the OxC-0.1 group showed greater inhibition on acetylcholine-induced relaxation compared with that in the C-0.1 hamsters. It was concluded that OxC was much more hypercholesterolaemic and atherogenic than cholesterol.
authors.affiliation: Food and Nutritional Sciences Programme, Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.
authors.forename: Chi Ho
authors.initials: CH
authors.lastname: Ng
authors.forename: Xiao
authors.initials: X
authors.lastname: Qiang Yao
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Chen
mesh_terms: D000109:Acetylcholine; D000818:Animals; D001011:Aorta / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D050197:Atherosclerosis / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D002784:Cholesterol / Q000032:analysis / Q000097:blood; D002788:Cholesterol Esters / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000008:administration & dosage*; D008078:Cholesterol, LDL / Q000097:blood; D006224:Cricetinae; D005243:Feces / Q000737:chemistry; D006937:Hypercholesterolemia / Q000209:etiology* / Q000378:metabolism; D066298:In Vitro Techniques; D007653:Ketocholesterols / Q000008:administration & dosage*; D008099:Liver / Q000737:chemistry; D008647:Mesocricetus; D011897:Random Allocation; D013261:Sterols / Q000032:analysis; D014280:Triglycerides / Q000097:blood; D014665:Vasodilator Agents
chemical_list: D002788:Cholesterol Esters; D002791:Cholesterol, Dietary; D008078:Cholesterol, LDL; D007653:Ketocholesterols; D013261:Sterols; D014280:Triglycerides; D014665:Vasodilator Agents; D002784:Cholesterol; D000109:Acetylcholine