[Periodontal wound filling affected by aging in an in vitro model].
OBJECTIVE
Using human periodontal ligament cells (PDLC) and gingival fibroblasts (GF) , that were cultured in vitro for a long period of time to simulate the aging cells, we evaluated the influence of aging on periodontal healing in an in vitro wound model.


METHODS
Human PDLC and GF were cultured in vitro over to 12 passages as aging cells for experiment, the same type of cells that were cultured under 5 passages as younger for control. The in vitro wound models were mechanically created in subconfluent cultures of this 4 types of cells, by removing a 7 mm wide band of the cell layer respectively. Wounded cultures were then incubated for 2, 6, 9 days in the same media. Slides were fixed, stained with crystal violet and cell filling area within the wound boundaries were quantified by computer-assisted histomorphometry. Statistical analysis was performed by SAS6.12 software package to determine the differences between the time points and groups.


RESULTS
The ability of filling wound of aging PDLC and GF decreased, there was significant difference (P<0.05) at day 6, 9 between the experimental and control groups. There was difference between GF and PDLC in filling wound in vitro over 9 days of healing period in both groups, but only the experimental group showed significant difference (P<0.05) at 9th day after wound creating.


CONCLUSIONS
The ability of filling wound of aging PDLC and GF were weaker then that of the younger. Within the limits of the present study, data analysis suggests that aging may impair the healing characterization of PDLC and GF.
Yin
Yuan-zheng
YZ
Department of Periodontics, College of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China. yyz99@citiz.net
D000375:Aging; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology*; D014945:Wound Healing