Taking it step by step: mechanistic insights from structural studies of ubiquitin/ubiquitin-like protein modification pathways.
Ubiquitin (Ub) and ubiquitin-like (Ubl) proteins regulate a diverse array of cellular pathways through post-translational attachment to protein substrates. Ub/Ubl-mediated signaling is initiated through E1, E2, and E3-mediated conjugation, transduced by proteins that recognize Ub/Ubl-modified substrates, and terminated by proteases which remove the Ub/Ubl from the substrate. Recent structural studies have elucidated mechanisms pertinent to Ub/Ubl conjugation, recognition, and deconjugation, highlighting essential steps during Ub/Ubl modification that illustrate common and divergent mechanistic themes within this important process.
Capili
Allan D
AD
Structural Biology Program, Sloan-Kettering Institute, 1275 York Ave., New York, NY 10065, USA.
Lima
Christopher D
CD
D008958:Models, Molecular; D011487:Protein Conformation; D025801:Ubiquitin / Q000737:chemistry / Q000378:metabolism*
D025801:Ubiquitin