title: Direct selection of human genomic loci by microarray hybridization.
abstract: We applied high-density microarrays to the enrichment of specific sequences from the human genome for high-throughput sequencing. After capture of 6,726 approximately 500-base 'exon' segments, and of 'locus-specific' regions ranging in size from 200 kb to 5 Mb, followed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection targets. These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic regions.
authors.affiliation: NimbleGen Systems Inc., 1 Science Court, Madison, Wisconsin 53711, USA. talbert@nimblegen.com
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Albert
authors.forename: Michael N
authors.initials: MN
authors.lastname: Molla
authors.forename: Donna M
authors.initials: DM
authors.lastname: Muzny
authors.forename: Lynne
authors.initials: L
authors.lastname: Nazareth
authors.forename: David
authors.initials: D
authors.lastname: Wheeler
authors.forename: Xingzhi
authors.initials: X
authors.lastname: Song
authors.forename: Todd A
authors.initials: TA
authors.lastname: Richmond
authors.forename: Chris M
authors.initials: CM
authors.lastname: Middle
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Rodesch
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Packard
authors.forename: George M
authors.initials: GM
authors.lastname: Weinstock
authors.forename: Richard A
authors.initials: RA
authors.lastname: Gibbs
mesh_terms: D019313:BRCA1 Protein / Q000235:genetics; D045744:Cell Line, Tumor; D015723:Gene Library; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D019313:BRCA1 Protein; D009838:Oligodeoxyribonucleotides