title: Regulation of pyrimidine formation in Pseudomonas oryzihabitans.
abstract: The regulation of pyrimidine formation in the opportunistic human pathogen Pseudomonas oryzihabitans was investigated at the level of enzyme synthesis and at the level of activity for the pyrimidine biosynthetic pathway enzyme aspartate transcarbamoylase. Although pyrimidine supplementation of succinate-grown P. oryzihabitans cells produced little effect on the de novo pyrimidine biosynthetic pathway enzyme activities, pyrimidine limitation experiments undertaken using an orotidine 5'-monophosphate decarboxylase mutant strain isolated from P. oryzihabitans ATCC 43272 indicated that repression of enzyme synthesis by pyrimidines was occurring. Following pyrimidine limitation of the succinate-grown decarboxylase mutant strain cells, aspartate transcarbamoylase and dihydroorotase activities were found to increase by about 3-fold while dihydroorotate dehydrogenase and orotate phosphoribosyltransferase activities were also observed to increase relative to their activities in the mutant strain cells grown on excess uracil. At the level of enzyme activity, aspartate transcarbamoylase in P. oryzihabitans was strongly inhibited by pyrophosphate, ADP, ATP and GTP in the presence of saturating substrate concentrations.
authors.affiliation: Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA. thomas.west@sdstate.edu
authors.forename: Thomas P
authors.initials: TP
authors.lastname: West
mesh_terms: D001221:Aspartate Carbamoyltransferase / Q000378:metabolism; D004080:Dihydroorotase / Q000378:metabolism; D000090722:Dihydroorotate Dehydrogenase; D004794:Enzyme Repression; D009962:Orotate Phosphoribosyltransferase / Q000378:metabolism; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D011743:Pyrimidines / Q000096:biosynthesis*; D014498:Uracil / Q000378:metabolism
chemical_list: D000090722:Dihydroorotate Dehydrogenase; D011743:Pyrimidines; D014498:Uracil; D044925:Oxidoreductases Acting on CH-CH Group Donors; D001221:Aspartate Carbamoyltransferase; D009962:Orotate Phosphoribosyltransferase; D004080:Dihydroorotase; D009964:Orotidine-5'-Phosphate Decarboxylase; C030986:pyrimidine