[Changes in the expression of voltage-dependent ca2+ channels in asthenospermia].
OBJECTIVE
To study the relationship between the expression of voltage-dependent Ca2+ channels (VDCCs) cal mRNA and asthenospermia.


METHODS
Based on the WHO criteria, we filtered the donated semen by computer-aided sperm analysis (CASA), optimized the ejaculated sperm by discontinuous Percoll grads centrifugation, and examined and quantitated the multitype VDCCs alpha1 mRNA expressions in the sperm of normal men and asthenospermia patients using reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
Compared with the normal sperm, there were significant differences among the expressions of alpha1B (39.40 +/- 9.47), alpha1C (48.30 +/- 11.60), alpha1E (3.20 +/- 0.78), alpha1G (8.40 +/- 2.03) and alpha1H (5.70 +/- 1.47) mRNA messages in asthenospermia (P < 0.01).


CONCLUSIONS
The abnormal expression of L-type and/or non-L-type VDCCs' mRNAs may be one of the causes of asthenospermia.
Li
Lu
L
Department of Urology, Tongji Hospital, Tongii Medical College, Huazhong University of Science & Technology, Wu-han, Hubei 430030, China.
Liu
Ji-hong
JH
D053627:Asthenozoospermia / Q000235:genetics / Q000473:pathology*; D015220:Calcium Channels / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D008297:Male; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012661:Semen / Q000378:metabolism; D013081:Sperm Motility / Q000235:genetics
D015220:Calcium Channels; D020033:Protein Isoforms; D012333:RNA, Messenger