title: Differentiation of 1-O-alk-1'-enyl-2-acyl and 1-O-alkyl-2-acyl glycerophospholipids by multiple-stage linear ion-trap mass spectrometry with electrospray ionization.
abstract: We described linear ion-trap mass spectrometric approaches applying MS(3) and MS(4) toward to the structural characterization of 1-O-alk-1'-enyl-2-acyl-, 1-O-alkyl-2-acyl-, and diacyl-glycerophospholipids (GPL) as the [M - H](-) ions desorbed by ESI in negative-ion mode. Further dissociation of the [lM - H - R(2)CO(2)H - polar head group](-) ions from the [M - H](-) ions of GPL that have undergone the consecutive losses of the fatty acid substituent at sn-2 and the polar head group readily gives the structural information of the radyl group at sn-1, resulting in structural differentiation among the 1-O-alk-1'-enyl-2-acyl-, 1-O-alkyl-2-acyl, and diacyl-glycerolphospholipid molecules. The distinction between a 1-O-alk-1'-enyl-2-acyl- and a 1-O-alkyl-2-acyl-GPL is based on the findings that the MS(3) (or MS(4)) spectrum of the [M - H - R(2)CO(2)H - polar head group](-) ion from the former compound is dominated by the alkenoxide anion that represents the radyl moiety at sn-1, while the spectrum from the latter compound is dominated by the ion at m/z 135 arising from further loss of the 1-O-alkyl group as an alcohol. Another important notion is that the optimal collision energy required for acquiring the former spectrum is significantly lower than that required for obtaining the latter spectrum. Using the approaches, we are able to reveal the structures of several isobaric isomers in GPL mixtures of biological origin. Because the [M - H](-) ions are readily formed by various GPL classes (except glycerophosphocholine) in the negative-ion mode, these mass spectrometric approaches should have broad application in the structural identification of GPLs.
authors.affiliation: Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid Research, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA. fhsu@im.wustl.edu
authors.forename: Fong-Fu
authors.initials: FF
authors.lastname: Hsu
authors.forename: John
authors.initials: J
authors.lastname: Turk
mesh_terms: D000818:Animals; D018320:Leishmania major / Q000737:chemistry / Q000378:metabolism; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D010712:Phosphatidic Acids / Q000737:chemistry*; D010955:Plasmalogens / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014346:Trypanosoma brucei brucei / Q000737:chemistry / Q000378:metabolism
chemical_list: D008563:Membrane Lipids; D010712:Phosphatidic Acids; D010955:Plasmalogens