Limits of a localized magnetic resonance spectroscopy assay for ex vivo myocardial triacylglycerol.
Localized magnetic resonance spectroscopy (LMRS) promises a powerful non-invasive means to determine myocardial triacylglycerol (TAG) in a clinical setting. Here, the linearity, specificity, robustness, precision, and accuracy of an ex vivo mouse-heart LMRS TAG assay are assessed by quantifying the spatial, spectral, and relaxation-induced uncertainties. The protocol, which is based on localization by adiabatic selective refocusing (LASER) using frequency offset corrected inversion (FOCI) pulses, alternating gradient polarity, and simple post-processing, is shown to have good characteristics. The presented protocol has a benchmark, phantom-based, accuracy of 3%, and when applied to ex vivo mouse hearts the accuracy is 6%, making the LMRS assay comparable to the typical destructive bioanalytical assay.
O'Connor
Robert D
RD
Department of Radiology, Washington University, Saint Louis, MO 63110, USA.
Gropler
Robert J
RJ
Peterson
Linda
L
Schaffer
Jean
J
Ackerman
Joseph J H
JJ
D000818:Animals; D009202:Cardiomyopathies / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D009206:Myocardium / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014280:Triglycerides / Q000032:analysis* / Q000378:metabolism
D014280:Triglycerides