Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags.
The characterization of expressed sequence tags (ESTs) generated from a cDNA library of Leishmania (Leishmania) amazonensis amastigotes is described. The sequencing of 93 clones generated new L. (L.) amazonensis ESTs from which 32% are not related to any other sequences in database and 68% presented significant similarities to known genes. The chromosome localization of some L. (L.) amazonensis ESTs was also determined in L. (L.) amazonensis and L. (L.) major. The characterization of these ESTs is suitable for the genome physical mapping, as well as for the identification of genes encoding cysteine proteinases implicated with protective immune responses in leishmaniasis.
Gentil
Luciana Girotto
LG
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, 04023-062, Brasil.
Lasakosvitsch
Fernanda
F
Silveira
José Franco da
JF
Santos
Márcia Regina Machado dos
MR
Barbiéri
Clara Lúcia
CL
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics*; D020224:Expressed Sequence Tags; D007891:Leishmania / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data
D018076:DNA, Complementary; D016054:DNA, Protozoan; D003546:Cysteine Endopeptidases