[Isolation, idetification of 1,2, 4-trichlorobenzene-degrading strain Pseudomonas nitroreducens J5-1 and cloning of chlorocatechol 1,2-dioxygenase gene].
A bacterium capable of utilizing 1,2,4-trichlorobenzene as sole carbon source was isolated from the polluted soil sample. This baterium was identified as Pseudomonas nitroreducens according to its physiological & biochemical analysis and its 16S rDNA sequence (GenBank Accession No. EF107515). When the initial concentration of 1,2,4-TCB is 400 mg/L, J5-1 can achieve a maximum degradation rate of 90%. When the initial concentration of 1,2,4-TCB is 20 mg/L, the effect of degradation is the best. Degradation of 1,2,4-TCB by strain J5-1 obeys the first order dynamics. The total gene of chlorocatechol 1,2-dioxygenase was cloned from genomic DNA of J5-1.
Song
Lei
L
State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China. L-song02@mails.tsinghua.edu.cn
Wang
Hui
H
Jiang
Jian
J
Gao
Jing-Si
JS
Shi
Han-Chang
HC
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002722:Chlorobenzenes / Q000378:metabolism*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D049308:Dioxygenases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013379:Substrate Specificity
D001426:Bacterial Proteins; D002722:Chlorobenzenes; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C009947:1,2,4-trichlorobenzene; D049308:Dioxygenases; C044173:chlorocatechol 1,2-dioxygenase