Gene expression in mammalian cells using BacMam, a modified baculovirus system.
BacMams are modified baculoviruses that contain mammalian expression cassettes for gene delivery and expression in mammalian cells. The BacMam system combines the advantages of viral transient expression, ease in generation, and a wide cell tropism. It enables rapid, facile, and flexible gene over-expression experiments to be performed in a variety of mammalian cell lines. Conversion of baculovirus vectors to BacMam vectors involves replacement of the viral specific expression cassette with a mammalian expression cassette or the addition of a mammalian expression cassette. Viruses are produced using standard methods in a few weeks. Mammalian cells transduced with the BacMam viruses have been routinely used as substitutes for stable cell lines.
Fornwald
James A
JA
Department of Gene Expression and Protein Biochemistry, GlaxoSmithKline, King of Prussia, PA, USA.
Lu
Quinn
Q
Wang
Dayuan
D
Ames
Robert S
RS
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D005434:Flow Cytometry; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D009036:Moths / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection / Q000379:methods
D004279:DNA, Viral; D011993:Recombinant Fusion Proteins; D008156:Luciferases