title: Comparison of resolution in microemulsion EKC and MEKC employing suppressed electroosmosis: application to bisphenol-A-diglycidyl ether and its derivatives.
abstract: The resolution (R(s)) of hydrophobic analytes in microemulsion EKC (MEEKC) and MEKC with suppressed electroosmosis was investigated using bisphenol-A-diglycidyl ether and its derivatives (BADGEs) as test analytes. Separation scales were compared using our equation for the resolution, R(s)= (square rootN/4)(alpha-1)/(1+K(2)),where k is the retention factor, alpha the selectivity (alpha = k(2)/k(1) for k(2) > or = k(1)>0), and N the average efficiency. At given concentrations of SDS and organic cosolvent in the buffer, in comparison with MEKC, MEEKC was found to provide better resolution of BADGEs, mainly due to the significantly smaller k in MEEKC, but not the greater alpha in MEEKC, while a comparable range of N. Significantly improved resolution of BADGEs was obtained with increase in the concentration of organic cosolvent in the MEEKC and MEKC buffers, while small change in R(s) with the SDS concentration in a range of 100-180 mM. In addition, a decrease in temperature or voltage resulted in slightly better R(s).
authors.affiliation: Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Kieatsuda
authors.initials: K
authors.lastname: Poouthree
authors.forename: Wasura
authors.initials: W
authors.lastname: Soonthorntantikul
authors.forename: Natchanun
authors.initials: N
authors.lastname: Leepipatpiboon
authors.forename: Amorn
authors.initials: A
authors.lastname: Petsom
authors.forename: Thumnoon
authors.initials: T
authors.lastname: Nhujak
mesh_terms: D000465:Algorithms; D001559:Benzhydryl Compounds; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D053841:Electroosmosis / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004655:Emulsions; D004852:Epoxy Compounds / Q000737:chemistry / Q000302:isolation & purification*; D008823:Micelles; D009930:Organic Chemicals / Q000737:chemistry; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D002021:Buffers; D004655:Emulsions; D004852:Epoxy Compounds; D008823:Micelles; D009930:Organic Chemicals; D012967:Sodium Dodecyl Sulfate; D012997:Solvents; C019273:bisphenol A diglycidyl ether; D000098826:Bisphenol A Compounds