title: Epidermal growth factor- and stress-induced loss of gap junctional communication is mediated by ERK-1/ERK-2 but not ERK-5 in rat liver epithelial cells.
abstract: Extracellular signal-regulated kinases (ERK) 1 and 2 as well as ERK-5 were previously suggested to phosphorylate connexin-43 and to contribute to the modulation of gap junctional intercellular communication (GJC). Exposure of rat liver epithelial cells to epidermal growth factor (EGF) or the redox cycling and alkylating agent menadione resulted in phosphorylation of connexin-43 and loss in GJC, both of which were abrogated by pharmacological inhibitors of ERK-1/2 activation, if used in concentrations that selectively abrogate phosphorylation of ERK-1/2 but not of ERK-5. Thus, EGF- or menadione-induced loss of GJC is mediated by ERK-1/2 but not ERK-5 in rat liver epithelial cells.
authors.affiliation: Institut für Biochemie und Molekularbiologie I, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany.
authors.forename: Kotb
authors.initials: K
authors.lastname: Abdelmohsen
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Sauerbier
authors.forename: Niloofar
authors.initials: N
authors.lastname: Ale-Agha
authors.forename: Juliane
authors.initials: J
authors.lastname: Beier
authors.forename: Philippe
authors.initials: P
authors.lastname: Walter
authors.forename: Stefanie
authors.initials: S
authors.lastname: Galban
authors.forename: Dominik
authors.initials: D
authors.lastname: Stuhlmann
authors.forename: Helmut
authors.initials: H
authors.lastname: Sies
authors.forename: Lars-Oliver
authors.initials: LO
authors.lastname: Klotz
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D018031:Connexin 43 / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology*; D017629:Gap Junctions / Q000187:drug effects / Q000502:physiology*; D008099:Liver / Q000166:cytology; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D048054:Mitogen-Activated Protein Kinase 7 / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D024483:Vitamin K 3 / Q000494:pharmacology
chemical_list: D018031:Connexin 43; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor; D024483:Vitamin K 3; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D048054:Mitogen-Activated Protein Kinase 7