Early and late gene expression in pepper huasteco yellow vein virus.
Viral infections usually take place in an orderly manner and can be divided into at least two phases: an early and a late stage. In geminiviruses, plant viruses with a circular, single-stranded DNA genome, expression of viral genes involves complex regulation strategies that suggest the existence of a pattern of temporal gene expression. In this work, the transcription of pepper huasteco yellow vein virus (PHYVV) genes was studied. Green fluorescent protein replacements and RT-PCR analyses were used to monitor PHYVV gene expression chronologically in suspension cells and plant tissue. A model is proposed to describe the order of geminivirus gene expression, where the genes that encode Rep, TrAP and REn are expressed during an early stage of infection. The genes that encode the coat protein and the nuclear shuttle protein are expressed during the late stage of infection.
Shimada-Beltrán
Harumi
H
Departamento de Ingeniería Genética, Cinvestav-Unidad Irapuato, km 9.6 Libramiento Norte, Apartado Postal 629, 36500 Irapuato, GTO, Mexico.
Rivera-Bustamante
Rafael F
RF
Departamento de Ingeniería Genética, Cinvestav-Unidad Irapuato, km 9.6 Libramiento Norte, Apartado Postal 629, 36500 Irapuato, GTO, Mexico.
D019677:Artificial Gene Fusion; D053839:Begomovirus / Q000235:genetics / Q000502:physiology*; D015967:Gene Expression Regulation, Viral; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D010944:Plants / Q000821:virology; D012367:RNA, Viral / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014158:Transcription, Genetic / Q000502:physiology*
D012367:RNA, Viral; D049452:Green Fluorescent Proteins