[Functional genomics studies of Salvia miltiorrhiza III. analyze of metallothionein (MT-2) genes].
OBJECTIVE
To study the metallothionein genes of Salvia miltiorrhiza through bioinformatics and characterization of tissue expression in regenerated shoots.


METHODS
Metallothionein genes were obtained by cDNA microarray analyze. BLAST was used for align, ORF finder software was used to find open reading frame, Prosite database was used to analyze the protein. Semi-quantitative RT-PCR method was used to detect the gene expression level.


RESULTS
Two metallothionein genes were obtained which were contained a deduced amino acid sequence of 80 and 79 residues, named as SmMT-2a and SmMT-2b, they had a homology of 71.25%. Semiquantitative RT-PCR indicated that metallothionein genes were expressed in all tissues such as root, stem and leaf in regenerated shoots, while the expression level was higher in leaf than in root and stem.


CONCLUSIONS
It was the first time that metallothionein genes were obtained from S. miltiorrhiza. It provides a good basis for further functional study of S. miltiorrhiza.
Cui
Guang-Hong
GH
Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China.
Mao
Ying
Y
Huang
Lu-Qi
LQ
Yuan
Yuan
Y
Wang
Xue-Yong
XY
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D023281:Genomics; D008668:Metallothionein / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D010946:Plants, Medicinal / Q000235:genetics* / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D008668:Metallothionein