Recombinant proteins and peptides as tools for studying IgE reactivity with low-molecular-weight glutenin subunits in some wheat allergies.
Two genes of wheat low-molecular-weight glutenin subunits (LMW-GS), B16 and P73, were cloned and expressed in E. coli. They were homologous to proteins encoded respectively at Glu-B3 and Glu-D3 loci. The N-terminal and C-terminal halves of B16 (NB16 and B16C) and the two chimeras combining the halves of the two genes (B16-P73 and P73- B16) were also expressed. All these constructs were compared for their reactivity with IgE from 24 patients suffering from different forms of wheat allergies. The results confirmed that LMW-GSs bound IgE in all adult allergies tested. Strong differences in reactivity between all the constructs were observed. They were disease-dependent. In wheat-dependent exercise-induced anaphylaxis (WDEIA), the reactivity of the constructs depended partly on common epitopes with omega-5 gliadins but also on differences in molecule conformation. The presence of NB16 in the constructs greatly influenced their IgE reactivity.
Snégaroff
Jacques
J
UMR206 Chimie Biologique, AgroParisTech, INRA, Thiverval-Grignon, France. Jacques.Snegaroff@grignon.inra.fr.
Branlard
Gérard
G
Bouchez-Mahiout
Isabelle
I
Laudet
Béatrice
B
Tylichova
Martina
M
Chardot
Thierry
T
Pecquet
Catherine
C
Choudat
Dominique
D
Raison-Peyron
N
N
Vigan
M
M
Kerre
S
S
Laurière
Michel
M
D000595:Amino Acid Sequence; D000707:Anaphylaxis / Q000276:immunology; D002678:Chimera; D004926:Escherichia coli; D015444:Exercise; D005983:Glutens / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D021182:Wheat Hypersensitivity / Q000175:diagnosis / Q000276:immunology*
D010940:Plant Proteins; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D005983:Glutens; C037302:glutenin