title: Epstein-Barr virus (EBV) infection in B-cell non-Hodgkin's lymphomas in children: virus latency and its correlation with CD21 and CD23 molecules.
abstract: Epstein Barr virus (EBV) infection of human B lymphocytes in vitro results in immortalisation of the cells and augmented membranous expression of numerous B-cell activation molecules, including CD23. Other studies demonstrated that only those B lymphocytes which carry the surface CD21 (EBV receptor) become transformation-competent. Inspired by the relatively unclear relations between expression of EBV and those of CD21 and CD23 in in vivo conditions we have decided to define correlations between tissue markers of EBV and of CD21 and CD23 molecules in B-cell non-Hodgkin's lymphomas (NHLs) in children. The studies were performed on an archival tissue material originating from children with B-cell NHLs (n=26) using immunocytochemical techniques, in situ hybridisation, and PCR. Our studies confirmed the latent phase of EBV infection in all of the EBV-positive patients. Viral proteins as well as viral RNAs (EBERs) was found both in the cytoplasm, in cell nuclei and in cell membranes of mainly the transformed lymphocytes B. Expression of the latent proteins (EBNA2 and LMP1) and that of EBERs in B-cell NHLs was significantly higher as compared to children with nonneoplastic lesions. The studies demonstrated reciprocally positive correlations between expressions of CD21 and CD23 in our children, but no correlation could be demonstrated between expression of EBV tissue markers and that of CD21 and/or CD23. Positive correlation was confirmed between expression of EBNA2 and LMP1 as well as between expression of the two proteins and EBERs in B-cell NHLs. Our studies have shown mainly latency III pattern of EBV. We have also demonstrated a novel form of EBV latency with no EBERs expression. The high detectability of EBV-positive cases both in the group of B-cell NHLs (77%), and in the group with non-neoplastic lesions (64%) suggested that only more pronounced tissue expression of EBV markers in B-cell NHLs as compared to the non-neoplastic material may point to a potential role of EBV in pathogenesis of lymphoma in this group of population in our country.
authors.affiliation: Department of Histology and Embryology, University of Medical Sciences, Poznan, Poland. akasprza@amp.edu.pl
authors.forename: Aldona
authors.initials: A
authors.lastname: Kasprzak
authors.forename: Rafal
authors.initials: R
authors.lastname: Spachacz
authors.forename: Jacek
authors.initials: J
authors.lastname: Wachowiak
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Stefanska
authors.forename: Maciej
authors.initials: M
authors.lastname: Zabel
mesh_terms: D000293:Adolescent; D001402:B-Lymphocytes / Q000821:virology*; D014408:Biomarkers, Tumor / Q000096:biosynthesis; D002648:Child; D002675:Child, Preschool; D015897:Comorbidity; D004268:DNA-Binding Proteins / Q000097:blood; D004720:Endonucleases; D020031:Epstein-Barr Virus Infections / Q000097:blood* / Q000453:epidemiology; D005260:Female; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D016393:Lymphoma, B-Cell / Q000453:epidemiology / Q000378:metabolism* / Q000821:virology*; D008297:Male; D009687:Nuclear Proteins / Q000097:blood; D011044:Poland / Q000453:epidemiology; D017464:Receptors, Complement 3d / Q000096:biosynthesis*; D017455:Receptors, IgE / Q000096:biosynthesis*; D015534:Trans-Activators / Q000097:blood; D014763:Viral Matrix Proteins / Q000097:blood; D014764:Viral Proteins / Q000097:blood; D017735:Virus Latency
chemical_list: C063676:BZLF1 protein, Herpesvirus 4, Human; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D009687:Nuclear Proteins; D017464:Receptors, Complement 3d; D017455:Receptors, IgE; D015534:Trans-Activators; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D004720:Endonucleases; C095221:SND1 protein, human