[Inhibitory effect of polysaccharides on the six-alpha-helix bundle formation of HIV gp41 protein].
OBJECTIVE
To compare the in vitro inhibitory effect of expolysaccharides from Streptomyces, polysaccharides of Ganoderma lucidum and rice bran on six-alpha-helix bundle formation of HIV gp41 protein.


METHODS
The amount of six-alpha-helix bundle formed in the presence of N36 and C34 was tested by ELISA in response to treatments with different doses of polysaccharides.


RESULTS
Expolysaccharides from Streptomyces potentially inhibited six-alpha-helix bundle formation with the effective concentration (IC(50)) of 145.48-/+7.25 mg /L. Polysaccharides of Ganoderma lucidum and rice bran showed no effect on the six-alpha-helix bundle formation.


CONCLUSIONS
Expolysaccharides from Streptomyces can inhibit the six-alpha-helix bundle formation of HIV gp41, whereas polysaccharides of Ganoderma lucidum and rice bran do not exhibit such activity.
Wen
Xiao-Yun
XY
School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. wenxy@fimmu.com
Wu
Shao-Yu
SY
Xu
Wei
W
Lv
Li
L
Liu
Shu-Wen
SW
Rao
Jin-Jun
JJ
Zhang
Jia-Jie
JJ
Wang
Guang-Fa
GF
Wan
Shan-He
SH
Wu
Shu-Guang
SG
D015700:HIV Envelope Protein gp41 / Q000737:chemistry*; D007700:Kinetics; D012275:Oryza / Q000737:chemistry; D011134:Polysaccharides / Q000494:pharmacology*; D017433:Protein Structure, Secondary / Q000187:drug effects; D020940:Reishi / Q000737:chemistry; D013302:Streptomyces / Q000737:chemistry
D015700:HIV Envelope Protein gp41; D011134:Polysaccharides