Centromeric DNA hypomethylation as an epigenetic signature discriminates between germ and somatic cell lineages.
Germ cells have unique features strikingly distinguishable from somatic cells. The functional divergence between these two cell lineages has been postulated to result from epigenetic mechanisms. Here we show that the chromosomal centric and pericentric (C/P) regions in male and female germline cells are specifically DNA-hypomethylated, despite the hypermethylation status in somatic cells. In multipotent germline stem cells, the C/P region was initially hypomethylated and then shifted to the hypermethylation status during differentiation into somatic lineage in vitro. Moreover, the somatic-type hypermethylation pattern was maintained in the somatic cell-derived nuclear transfer embryos throughout preimplantation development. These results imply that the identity of germ cell lineage may be warranted by the hypomethylation status of the C/P region as an epigenetic signature.
Yamagata
Kazuo
K
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan.
Yamazaki
Taiga
T
Miki
Hiromi
H
Ogonuki
Narumi
N
Inoue
Kimiko
K
Ogura
Atsuo
A
Baba
Tadashi
T
D000818:Animals; D019070:Cell Lineage; D065150:Cellular Reprogramming; D002503:Centromere / Q000235:genetics* / Q000378:metabolism*; D018899:CpG Islands; D019175:DNA Methylation; D004276:DNA, Satellite / Q000378:metabolism; D010064:Embryo Implantation; D004622:Embryo, Mammalian / Q000378:metabolism; D044127:Epigenesis, Genetic; D005260:Female; D005307:Fertilization in Vitro; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D053652:Nuclear Transfer Techniques; D009928:Organ Specificity; D010063:Ovum / Q000166:cytology / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism
D004276:DNA, Satellite