PCR versus hybridization for detecting virulence genes of enterohemorrhagic Escherichia coli.
We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. Both methods showed 100% of the chromosomal and phage genes: eae, stx, and stx2. PCR did not detect 4%-20% of hybridizable plasmid genes: hlyA, katP, espP, toxB, open reading frame (ORF) 1, and ORF2.
Gerrish
Robert S
RS
Idaho State University, Pocatello, Idaho, Pocatello 83209, USA.
Lee
James E
JE
Reed
June
J
Williams
Joel
J
Farrell
Larry D
LD
Spiegel
Kathleen M
KM
Sheridan
Peter P
PP
Shields
Malcolm S
MS
D018829:Adhesins, Bacterial / Q000235:genetics; D015139:Blotting, Southern / Q000379:methods; D054324:Enterohemorrhagic Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000235:genetics / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D022622:Shiga Toxin 1 / Q000235:genetics; D022641:Shiga Toxin 2 / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics*
D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D037521:Virulence Factors; C073819:eaeA protein, E coli