Subacute H2O2, but not poly(IC), upregulates dopamine D2 receptors in retinoic acid differentiated SH-SY5Y neuroblastoma.
Abnormalities of striatal dopaminergic neurotransmission play a significant role in the pathophysiology of central nervous system disorders such as movement disorders, addictions and schizophrenia. The striatum appears to be exposed to intrinsically high levels of oxidative stress (OS). Little is known, however, on the effect of OS on the regulation of the dopamine D2 receptor (DRD2), a key component of striatal dopaminergic neurotransmission. We report here on the effects of H2O2 (a canonical oxidant and non conventional messenger) and polyinosinic-polycytidylic acid (poly(IC) which elicits schizophrenia-like behaviors in newborn rodents and disrupts dopaminergic system development), on DRD2 levels in retinoic acid differentiated SH-SY5Y neuroblastoma. H2O2 elicited a significant increase in DRD2 mRNA and protein levels. Conversely, poly(IC) did not regulate DRD2 levels, although SH-SY5Y cells were confirmed to express TLR3 receptors. Under our conditions, H2O2, but not poly(IC), increased NFkappaB activation (as assessed by p65 nuclear translocation), which paralleled their effects on DRD2 levels regulation.
Larouche
Annie
A
Department of Psychiatry, Sherbrooke University, Sherbrooke, Québec, Canada.
Berube
Patrick
P
Sarret
Philippe
P
Grignon
Sylvain
S
D015153:Blotting, Western; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007369:Interferon Inducers / Q000494:pharmacology*; D016328:NF-kappa B / Q000096:biosynthesis / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress / Q000502:physiology; D011070:Poly I-C / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D017448:Receptors, Dopamine D2 / Q000187:drug effects*; D014212:Tretinoin / Q000494:pharmacology
D007369:Interferon Inducers; D016328:NF-kappa B; D016877:Oxidants; D012333:RNA, Messenger; D017448:Receptors, Dopamine D2; D014212:Tretinoin; D006861:Hydrogen Peroxide; D011070:Poly I-C