Diagnostic reverse-transcription polymerase chain reaction kit for filoviruses based on the strain collections of all European biosafety level 4 laboratories.
A network of European biosafety level 4 laboratories has designed the first industry-standard molecular assay for all filoviruses species, based on the strain collections of all participants. It uses 5 optimized L gene primers and 3 probes, as well as an internal control with a separate detection probe. Detection limits (probit analysis, 95% detection chance) were as follows: Zaire ebolavirus, 487 copies/mL of plasma; Sudan ebolavirus Maleo, 586 copies/mL; Sudan ebolavirus Gulu, 1128 copies/mL; Cote d'Ivoire ebolavirus, 537 copies/mL; Reston ebolavirus, 4546 copies/mL; Lake Victoria marburgvirus Musoke, 860 copies/mL; and Lake Victoria marburgvirus Ravn, 1551 copies/mL. The assay facilitates reliable detection or exclusion screening of filovirus infections.
Panning
Marcus
M
Bernhard-Nocht Institute for Tropical Medicine, Hamburg 20359, Germany.
Laue
Thomas
T
Olschlager
Stephan
S
Eickmann
Markus
M
Becker
Stephan
S
Raith
Sabine
S
Courbot
Marie-Claude Georges
MC
Nilsson
Mikael
M
Gopal
Robin
R
Lundkvist
Ake
A
di Caro
Antonino
A
Brown
David
D
Meyer
Hermann
H
Lloyd
Graham
G
Kummerer
Beate M
BM
Gunther
Stephan
S
Drosten
Christian
C
D000818:Animals; D002522:Chlorocebus aethiops; D017931:DNA Primers; D005060:Europe; D016563:Filoviridae / Q000235:genetics / Q000302:isolation & purification*; D018702:Filoviridae Infections / Q000517:prevention & control*; D000882:Haplorhini; D006801:Humans; D007753:Laboratories / Q000592:standards*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012449:Safety; D012680:Sensitivity and Specificity; D014709:Vero Cells
D017931:DNA Primers; D011933:Reagent Kits, Diagnostic