[Effect of liposomal transfection of antisense oligodeoxynucleotide on alpha-globin gene expression and proliferation of K562 cells].
The objective of study was to investigate the effect of liposomal transfection of antisense oligodeoxynucleotide (ASON) on alpha-globin gene expression and proliferation of K562 cells, to explore the new way of gene therapy in beta-thalassemia. Targeted ASON of alpha-globin was designed and synthesized, and compared with positive control [sense oligodeoxynucleotide (SON) group] and blank control. By liposomal transfection, ASON, SON was co-cultured with K562. The efficiency of transfection was assayed by fluorescence microscopy and flow cytometry (FCM), the alpha-globin gene expression of K562 was measured by real-time PCR, and the proliferation of K562 was determined by Cell Count Kit-8 assay. The results indicated that the highest efficiency was at 24 hours after liposomal transfection, the gene expression level of alpha-globin in ASON group was significantly lower than that in SON group and blank control (p < 0.01). The proliferation of K562 cells was obviously inhibited, meanwhile the above effect showed the dose-dependent manner. It is concluded that the liposomal transfection of ASON inhibits the alpha-globin gene expression of K562 cells, which may be the new target for gene therapy in beta-thalassemia.
Liu
Rong-Rong
RR
Department of Hematology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
Lai
Yong-Rong
YR
Ma
Jie
J
D049109:Cell Proliferation / Q000187:drug effects*; D015870:Gene Expression; D006801:Humans; D020014:K562 Cells; D008081:Liposomes / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D014162:Transfection; D055542:alpha-Globins / Q000378:metabolism*
D008081:Liposomes; D016376:Oligonucleotides, Antisense; D055542:alpha-Globins