title: Photo-cross-linking between polymers derivatized with photoreactive ruthenium-1,4,5,8-tetraazaphenanthrene complexes and guanine-containing oligonucleotides.
abstract: We have shown previously that complexes containing 1,4,5,8-tetraazaphenanthrene (TAP) ligands are able to form photoadducts with the guanine bases of DNA and oligonucleotides. In this work, we have exploited this specific photoreaction for carrying out photo-cross-linkings between guanine-containing oligonucleotides (G-ODNs) and biodegradable polymers derivatized with the photoreactive Ru(II) compounds. The aim in the future is to use these polymer conjugates as vectorizing agents of the metallic compounds inside the cells. Thus, photooxidizing Ru(II) complexes such as [Ru(TAP)3]2+ and [Ru(TAP)2phen]2+ (phen = 1,10-phenanthroline) have been derivatized by an oxyamine function to attach them, via an oxime ether linkage, to a soluble 6 or 80 kDa poly-[N-(2-hydroxyethyl)-l-glutamine] polymer that contains pendent aldehyde groups. It is demonstrated that the resulting Ru-labeled polymers exhibit photophysical properties and a photochemistry that are comparable with those of the free, nonattached complexes. The photo-cross-linkings with the G-ODNs are clearly detected by gel electrophoresis with the 6 kDa Ru conjugates upon illumination.
authors.affiliation: Organic Chemistry and Photochemistry, Université Libre de Bruxelles, CP 160/08, 50 Avenue F. D. Roosevelt, Brussels, Belgium.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Deroo
authors.forename: Veska
authors.initials: V
authors.lastname: Toncheva
authors.forename: Eric
authors.initials: E
authors.lastname: Defrancq
authors.forename: Cécile
authors.initials: C
authors.lastname: Moucheron
authors.forename: Etienne
authors.initials: E
authors.lastname: Schacht
authors.forename: Andrée
authors.initials: A
authors.lastname: Kirsch-De Mesmaeker
mesh_terms: D006147:Guanine / Q000737:chemistry*; D008024:Ligands; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry*; D010616:Phenanthrenes / Q000737:chemistry*; D010777:Photochemistry; D011108:Polymers / Q000737:chemistry*; D017975:Ruthenium Compounds / Q000737:chemistry*; D013053:Spectrophotometry
chemical_list: D008024:Ligands; D009841:Oligonucleotides; D010616:Phenanthrenes; D011108:Polymers; D017975:Ruthenium Compounds; D006147:Guanine