Evaluating yeast biosynthetic vacuolar transport.
Study of the lysosomal protein transport system has been facilitated through dissection of the analogous vacuolar protein sorting (VPS) pathway in Saccharomyces cerevisiae. Resident enzymes of the yeast vacuole are synthesized as inactive precursors and are cleaved to their mature forms upon delivery to this compartment. Quantitative assessment of this delivery can be achieved through the use of pulse-chase experiments monitoring the cleavage of zymogens to their mature forms. The experimental procedures for analysis of carboxypeptidase Y (CPY) and carboxypeptidase S (CPS) maturation are described.
Davies
Brian A
BA
Biochemistry and Molecular Biology Department, Mayo Clinic College of Medicine, Rochester, MN, USA.
Carney
Darren S
DS
Horazdovsky
Bruce F
BF
D001692:Biological Transport; D002268:Carboxypeptidases / Q000378:metabolism; D004705:Endocytosis; D005656:Fungal Proteins / Q000096:biosynthesis / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism*
D005656:Fungal Proteins; D002268:Carboxypeptidases