The increase in retinal cells proliferation induced by FGF2 is mediated by tyrosine and PI3 kinases.
Since 1973, multiple effects of basic fibroblast growth factor have been described in a large number of cells. These effects include proliferation, survival and differentiation. The aim of this work was to study the intracellular pathways involved in the basic fibroblast growth factor (FGF2) effect on rat retinal cells proliferation in vitro. Our data show that treatment with FGF2 increases proliferation in a concentration- and time-dependent manner. The effect of 25 ng/ml FGF2 was blocked by 10 microM genistein, a tyrosine kinase inhibitor and by 25 microM LY294002, a PI3 kinase inhibitor. The concomitant treatment with 0.3 microM chelerythrine chloride, a protein kinase C inhibitor, and 6.25 microM LY294002 also inhibited the effect of FGF2. Our results suggest that the proliferative effect of FGF2 on retinal cell cultures involves the activation of distinct kinases.
Guilarducci-Ferraz
Carla Valéria Vieira
CV
Programa de Neuroimunologia, Departamento de Neurobiologia, Instituto de Biologia, Universidade Federal Fluminense, Caxia Postal 100180, CEP 24.001-970 Niteroi, RJ, Brazil.
da Silva
Gustavo Mataruna
GM
Torres
Patrícia Maria Mendonça
PM
Dos Santos
Aline Araújo
AA
de Araújo
Elizabeth Giestal
EG
D000818:Animals; D053119:Benzophenanthridines / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D002867:Chromones / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D009025:Morpholines / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D012160:Retina / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology
D053119:Benzophenanthridines; D002867:Chromones; D016222:Fibroblast Growth Factor 2; D009025:Morpholines; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D047428:Protein Kinase Inhibitors; D011505:Protein-Tyrosine Kinases; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; C016299:chelerythrine