Association of the MMP-3 5A/6A gene polymorphism with multiple sclerosis in patients from Serbia.
Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in remodeling of the extracellular matrix. MMPs are suggested to play a role in the influx of inflammatory cells into the CNS, disruption of the blood brain barrier, and to degrade myelin in vitro. In this study, we have investigated the possible association of MMP-3 5A/6A gene polymorphism with MS susceptibility and/or severity in patients from Serbia. A total of 184 MS patients (150 RR, 34 SP) and 236 controls have been studied. Results show that the distribution of MMP-3 5A/6A genotype frequencies between MS patients and controls were not significantly different. In bout onset patients, carriers of MMP-3 6A/6A genotype had significantly higher mean MSSS values compared to the carriers of 5A allele (6.29+/-1.89 vs. 5.29+/-2.62, respectively, ANCOVA, p=0.01 Scheffe post-hoc test). In conclusion, our results indicate association of MMP-3 6A/6A genotype with significantly higher mean MSSS values. Thus, the obtained results suggest that it should be carefully considered during follow up of patients with MS. Further genetic and functional studies are needed to resolve the complex role of MMPs and their tissue inhibitors in MS pathology and/or regeneration.
Djurić
Tamara
T
Laboratory of Radiobiology and Molecular Genetics, Vinca Institute of Nuclear Sciences, 11000 Belgrade, Serbia. tamariska@vin.bg.ac.yu
Zivković
Maja
M
Stanković
Aleksandra
A
Dincić
Evica
E
Raicević
Ranko
R
Alavantić
Dragan
D
D000328:Adult; D002490:Central Nervous System / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology*; D004252:DNA Mutational Analysis; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005820:Genetic Testing; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000235:genetics*; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000201:enzymology* / Q000453:epidemiology / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D015022:Yugoslavia / Q000453:epidemiology
D005819:Genetic Markers; D019278:Matrix Metalloproteinase 3