Concentrations of methylated naphthalenes, anthracenes, and phenanthrenes occurring in Czech river sediments and their effects on toxic events associated with carcinogenesis in rat liver cell lines.
Alkylated polycyclic aromatic hydrocarbons (PAHs) are important environmental pollutants. In the present study, we determined levels of monomethylated naphthalenes (MeNap), phenanthrenes (MePhe), and anthracenes (MeAnt) in Czech river sediments. The levels of MePhe generally were lower than the concentrations of phenanthrene. In contrast, both MeNap and MeAnt were found at levels higher than their respective parent compounds in the majority of sampling sites. We then investigated their aryl hydrocarbon receptor (AhR)-mediated activity, accumulation of phosphorylated p53 protein, induction of expression of cytochrome P450 1A1 (CYP1A1), inhibition of gap junctional intercellular communication (GJIC), and effects on cell proliferation in rat liver cell models to evaluate the relative importance of these toxicity mechanisms of low-molecular-weight methylated PAHs. Methylated phenanthrene and anthracene compounds were weak inducers of AhR-mediated activity as determined both in a reporter gene assay system and by detection of the endogenous gene (Cyp1a1) induction. 2-Methylphenanthrene was the most potent AhR ligand. Contribution of MeAnt and MePhe to overall AhR-inducing potencies should be taken into account in PAH-contaminated environments. Nevertheless, their effects on AhR were not sufficient to modulate cell proliferation in a normal rat liver progenitor cell model system. These PAHs only had a marginal effect on p53 phosphorylation at high doses of 1-, 3-, and 9-MePhe as well as 1 MeAnt. On the other hand, both 2- and 9-MeAnt as well as all the MePhe under study were efficient inhibitors of GJIC, suggesting that these compounds might act as tumor promoters. In summary, inhibition of GJIC and partial activation of AhR seem to be the most prominent toxic effects of the methylated PAHs in the present study.
Vondrácek
Jan
J
Institute of Biophysics, Academy of Sciences of the Czech Republic, 61265 Brno, Czech Republic.
Svihálková-Sindlerová
Lenka
L
Pencíková
Katerina
K
Marvanová
Sona
S
Krcmár
Pavel
P
Ciganek
Miroslav
M
Neca
Jirí
J
Trosko
James E
JE
Upham
Brad
B
Kozubík
Alois
A
Machala
Miroslav
M
D000818:Animals; D000873:Anthracenes / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D018153:Czech Republic; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D017629:Gap Junctions / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D019015:Geologic Sediments / Q000737:chemistry*; D008099:Liver / Q000166:cytology / Q000473:pathology; D008745:Methylation; D009281:Naphthalenes / Q000633:toxicity; D010616:Phenanthrenes / Q000633:toxicity; D051381:Rats; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism; D045483:Rivers / Q000737:chemistry*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D000873:Anthracenes; D002273:Carcinogens; D004785:Environmental Pollutants; D009281:Naphthalenes; D010616:Phenanthrenes; D018336:Receptors, Aryl Hydrocarbon; D016159:Tumor Suppressor Protein p53; D019363:Cytochrome P-450 CYP1A1