Cloud point extraction coupled with HPLC-UV for the determination of phthalate esters in environmental water samples.
A method based on cloud point extraction was developed to determine phthalate esters including di-ethyl-phthalate (DEP), di-(2-ethylhexyl)-phthalate (DEHP) and di-cyclohexyl-phthalate (DCP) in environmental water samples using high-performance liquid chromatography separation and ultraviolet detection (HPLC-UV). The non-ionic surfactant Triton X-114 was chosen as extraction solvent. The parameters affecting extraction efficiency, such as concentrations of Triton X-114 and Na2SO4, equilibration temperature, equilibration time and centrifugation time were evaluated and optimized. Under the optimum conditions, the method can achieve preconcentration factors of 35, 88, 111 and detection of limits of 2.0, 3.8, 1.0 ng/ml for DEP, DEHP and DCP in 10-ml water sample, respectively. The proposed method was successfully applied to the determination of trace amount of phathalate esters in effluent water of the wastewater treatment plant and the lixivium of plastic fragments.
Wang
Ling
L
School of Chemistry and Chemical Engineering, Shan Dong University, Jinan 250100, China. qd_wling@sohu.com
Jiang
Gui-Bin
GB
Cai
Ya-Qi
YQ
He
Bin
B
Wang
Ya-Wei
YW
Shen
Da-Zhong
DZ
D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring; D017830:Octoxynol; D010795:Phthalic Acids / Q000032:analysis*; D011092:Polyethylene Glycols / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000032:analysis*
D010795:Phthalic Acids; D013431:Sulfates; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical; C012036:sodium sulfate; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40