Evaluation of two different potency tests for leptospirosis vaccine vax-SPIRAL.
Two different potency tests were evaluated, consisting in one-dilution used to determine 50% lethal dose (LD50) and four--dilution used to calculate 50% effective dose (ED50), respectively. Our experience has led to propose the four-dilution test for routine lot releasing.
Núñez
Juan Francisco
JF
Instituto Finlay, Ciudad de La Habana, Cuba. jnunez@finlay.edu.cu
Fajardo
Ester María
EM
Pérez
Elizabeth
E
Ontivero
Ivis
I
Silva
Deolinda
D
Muñoz
Pedro
P
D000818:Animals; D001428:Bacterial Vaccines / Q000592:standards* / Q000633:toxicity; D006224:Cricetinae; D003462:Cuba; D005500:Follow-Up Studies; D007919:Leptospira / Q000276:immunology* / Q000472:pathogenicity; D007922:Leptospirosis / Q000517:prevention & control*; D007928:Lethal Dose 50; D013997:Time Factors; D018675:Toxicity Tests
D001428:Bacterial Vaccines