Growth response of Salmonella typhimurium in the presence of natural and synthetic antimicrobials: estimation of MICs from three different models.
The aim of this study was to determine the MICs of 14 antimicrobials for Salmonella Typhimurium with three methods and to check the influence of experiment duration on the estimation of MICs. The growth of Salmonella Typhimurium in a brain heart infusion medium containing various concentrations of natural aromatic compounds, organic acids, or salts was monitored by absorbance measurements for 24 or 72 h. Three different ways of analyzing optical density (OD) curves were tested for the determination of MICs. Both quantitative methods gave similar MICs for most of the compounds. The semiquantitative method does not allow estimating the MIC for all compounds. Noticeable differences were found between MICs obtained for 24- or 72-h experiments, whatever the method used. The proposed methods and models can be used for the estimation of MICs from OD data. MICs could be used for a quantitative approach to Salmonella Typhimurium growth.
Guillier
L
L
AgroParisTech, UMR763 Bioadhésion et Hygiène des Aliments, 1 avenue des Olympiades, F-91300 Massy, France.
Nazer
A I
AI
Dubois-Brissonnet
F
F
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008954:Models, Biological; D012486:Salmonella typhimurium / Q000254:growth & development*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D003470:Culture Media