title: [Expressions of micro-calpain mRNA and protein in cerebral tissues of neonatal rats after hypoxic-ischemic brain damage].
abstract: OBJECTIVE
  To identify the expression of micro-calpain mRNA and protein in cerebral tissues of neonatal rats after hypoxic-ischemic brain damage (HIBD) and its role in the pathogenesis of HIBD.
  
  
  METHODS
  Seven-day-old Sprague-Dawley rats were randomly divided into seven groups: control group and groups with 0, 6, 12, 24, 48, and 72 hours of exposure to HIDB. Quantitative real-time fluroscent polymerase chain reaction and Western blot technique were employed to detect the expression of micro-calpain mRNA and its protein respectively.
  
  
  RESULTS
  The micro-calpain mRNA in the ipsilateral cerebral tissues began to increase 6 hours after the HIDB and reached peak at 24 hours, and then gradually decreased but remained in a higher level than that of the control group until at 72 hours (P < 0.05). The activated micro-calpain protein started to increase 6-12 hours after the HIDB (P < 0.05) and reached peak at 24 hours (P < 0.01). Higher levels of micro-calpain protein were maintained until 48 and 72 hours after the HIBD (P < 0.05).
  
  
  CONCLUSIONS
  The expressions of micro-calpain mRNA and protein increase with the HIBD. The micro-calpain may play a role in the pathogenesis of the HIBD.
authors.affiliation: Depratment of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Li-na
authors.initials: LN
authors.lastname: Chen
authors.forename: Yu-jia
authors.initials: YJ
authors.lastname: Yao
authors.forename: Da-peng
authors.initials: DP
authors.lastname: Chen
authors.forename: Yong-xiu
authors.initials: YX
authors.lastname: Chen
authors.forename: Xue
authors.initials: X
authors.lastname: Feng
mesh_terms: D000818:Animals; D002154:Calpain / Q000378:metabolism*; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D012333:RNA, Messenger; D002154:Calpain