title: Mapping the transcription start points of the Staphylococcus aureus eap, emp, and vwb promoters reveals a conserved octanucleotide sequence that is essential for expression of these genes.
abstract: Mapping the transcription start points of the eap, emp, and vwb promoters revealed a conserved octanucleotide sequence (COS). Deleting this sequence abolished the expression of eap, emp, and vwb. However, electrophoretic mobility shift assays gave no evidence that this sequence was a binding site for SarA or SaeR, known regulators of eap and emp.
authors.affiliation: Institute of Medical Microbiology and Hygiene, University of Saarland, 66421 Homburg/Saar, Germany. niamh.harraghy@unil.ch
authors.forename: Niamh
authors.initials: N
authors.lastname: Harraghy
authors.forename: Dagmar
authors.initials: D
authors.lastname: Homerova
authors.forename: Mathias
authors.initials: M
authors.lastname: Herrmann
authors.forename: Jan
authors.initials: J
authors.lastname: Kormanec
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D017124:Conserved Sequence; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011401:Promoter Regions, Genetic; D013211:Staphylococcus aureus / Q000235:genetics*; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D016326:Extracellular Matrix Proteins; D009838:Oligodeoxyribonucleotides; C088021:SarA protein, bacterial; D015534:Trans-Activators; D014157:Transcription Factors