title: Molecular diversity of 16S rRNA and gyrB genes in copper mines.
abstract: The molecular diversities of the microbial communities from four sites impacted by acid mine drainage (AMD) at Dexing Copper Mine in Jiangxi province of China were studied using 16S rRNA sequences and gyrB sequences. Of the four sampled sites, each habitat exhibited distinct geochemical characteristics and the sites were linked geographically allowing us to correlate microbial community structure to geochemical characteristics. In the present study, we examined the molecular diversity of 16S rRNA and gyrB genes from water at these sites using a PCR-based cloning approach. We found that the microbial community appears to be composed primarily of Proteobacteria, Acidobacteria, Actinobacteria, Nitrospira, Firmicutes, Chlorella and unknown phylotypes. Of clones affiliated with Nitrospira, Leptospirillum ferrooxidans, Leptospirillum ferriphilum and Leptospirillum group III were all detected. Principal-component analysis (PCA) revealed that the distribution of the microbial communities was influenced greatly by geochemical characteristics. The overall PCA profiles showed that the sites with similar geochemical characteristics had more similar microbial community structures. Moreover, our results also indicated that gyrB sequence analysis may be very useful for differentiating very closely related species in the study of microbial communities.
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan, 410083, China.
authors.forename: Huaqun
authors.initials: H
authors.lastname: Yin
authors.forename: Linhui
authors.initials: L
authors.lastname: Cao
authors.forename: Guanzhou
authors.initials: G
authors.lastname: Qiu
authors.forename: Dianzuo
authors.initials: D
authors.lastname: Wang
authors.forename: Laurie
authors.initials: L
authors.lastname: Kellogg
authors.forename: Jizhong
authors.initials: J
authors.lastname: Zhou
authors.forename: Xinxing
authors.initials: X
authors.lastname: Liu
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Dai
authors.forename: Jiannan
authors.initials: J
authors.lastname: Ding
authors.forename: Xueduan
authors.initials: X
authors.lastname: Liu
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D002681:China; D002708:Chlorella / Q000235:genetics / Q000302:isolation & purification*; D003001:Cloning, Molecular; D003300:Copper; D027081:DNA Gyrase / Q000235:genetics; D048028:DNA, Algal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D008906:Mining; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D048028:DNA, Algal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D003300:Copper; D027081:DNA Gyrase