title: Development of MAb-based immunochromatographic assay for cadmium from biological samples.
abstract: Cadmium (Cd) is a general environmental pollutant of increasing global concern. In 2005 the joint FAO/WHO Codex Alimentarius Commission proposed new international food legislation for low-level Cd contaminants. In this study we demonstrate the use of novel monoclonal antibody (MAb) to Cd-EDTA in an immunochromatography (IC) format for the quick testing for trace Cd. This IC device could detect 0.3 microg kg(-1) (0-3 ppb) Cd. Contaminated Zn, Mn, Mg, and Cu, which would interfere the measurement of Cd by cross reaction to the MAb, could be removed by using a column that could separate trace Cd from other heavy metals in the extract of brown rice.
authors.affiliation: Central Res. Inst. of Electr. Power Ind, 270-1194 Japan. k-sasaki@criepi.denken.or.jp
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sasaki
authors.forename: Kei
authors.initials: K
authors.lastname: Tawarada
authors.forename: Hirosi
authors.initials: H
authors.lastname: Okuhata
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Miyasaka
authors.forename: Akira
authors.initials: A
authors.lastname: Okuyama
authors.forename: Yukinao
authors.initials: Y
authors.lastname: Maruyama
authors.forename: Naoya
authors.initials: N
authors.lastname: Ohmura
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D002104:Cadmium / Q000032:analysis* / Q000276:immunology*; D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D012275:Oryza / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D002104:Cadmium