[Solubilization of components of outer membrane of Leptospira interrogans serovar mozdok by sodium desoxycholate and triton X-100 and evaluation of their immunogenic capacity and homologous and heterologous protection induced in hamsters].
Two detergents (triton X-100 and sodium desoxycholate) were evaluated as solubilizing agents of the components of the outer membrane of Leptospira Pomona serovar mozdok. It was obtained a greater yielding of proteins on applying the treatment with triton X-100. The variants formulated starting from the extracts obtained showed a high immunogenicity and capacity of homologous protection against the challenge in hamster.
Rivera
Yovania Fernández
YF
Laboratorio de Bioquímica, Instituto Finlay. yofernandez@finlay.edu.cu
Navarro
Yadira Pino
YP
Mazorra
Yisabel Aranguren
YA
Rodríguez
Andrés González
AG
Padrón
María de los Angeles
Mde L
Valdés
Yolanda
Y
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D006224:Cricetinae; D003840:Deoxycholic Acid / Q000494:pharmacology*; D004195:Disease Models, Animal; D007921:Leptospira interrogans / Q000276:immunology* / Q000472:pathogenicity; D041341:Leptospira interrogans serovar canicola / Q000276:immunology; D007922:Leptospirosis / Q000276:immunology / Q000517:prevention & control*; D007928:Lethal Dose 50; D017830:Octoxynol / Q000494:pharmacology*
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D003840:Deoxycholic Acid; D017830:Octoxynol