title: Oral administration of vitamin C prevents alveolar bone resorption induced by high dietary cholesterol in rats.
abstract: BACKGROUND
  A high-cholesterol diet stimulates alveolar bone resorption, which may be induced via tissue oxidative damage. Vitamin C reduces tissue oxidative damage by neutralizing free radicals and scavenging hydroxyl radicals, and its antioxidant effect may offer the clinical benefit of preventing alveolar bone resorption in cases of hyperlipidemia. We examined whether vitamin C could suppress alveolar bone resorption in rats fed a high-cholesterol diet.
  
  
  METHODS
  In this 12-week study, rats were divided into four groups: a control group (fed a regular diet) and three experimental groups (fed a high-cholesterol diet supplemented with 0, 1, or 2 g/l vitamin C). Vitamin C was provided by adding it to the drinking water. The bone mineral density of the alveolar bone was analyzed by microcomputerized tomography. As an index of tissue oxidative damage, the 8-hydroxydeoxyguanosine level in the periodontal tissue was determined using a competitive enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Hyperlipidemia, induced by a high-cholesterol diet, decreased rat alveolar bone density and increased the number of tartrate-resistant acid phosphatase-positive osteoclasts. The expression of 8-hydroxydeoxyguanosine was upregulated in the periodontal tissues. Intake of vitamin C reduced the effect of a high-cholesterol diet on alveolar bone density and osteoclast differentiation and decreased periodontal 8-hydroxydeoxyguanosine expression.
  
  
  CONCLUSIONS
  In the rat model, vitamin C suppressed alveolar bone resorption, induced by high dietary cholesterol, by decreasing the oxidative damage of periodontal tissue.
authors.affiliation: Department of Oral Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sanbe
authors.forename: Takaaki
authors.initials: T
authors.lastname: Tomofuji
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ekuni
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Azuma
authors.forename: Naofumi
authors.initials: N
authors.lastname: Tamaki
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000539:Alveolar Process / Q000473:pathology*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D001205:Ascorbic Acid / Q000008:administration & dosage*; D015519:Bone Density / Q000187:drug effects; D001862:Bone Resorption / Q000188:drug therapy* / Q000209:etiology / Q000517:prevention & control; D002784:Cholesterol / Q000009:adverse effects* / Q000097:blood; D004032:Diet / Q000009:adverse effects; D006949:Hyperlipidemias / Q000150:complications*; D008297:Male; D010010:Osteoclasts / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D002784:Cholesterol; D001205:Ascorbic Acid