title: Pressurized liquid extraction-assisted mussel cytosol preparation for the determination of metals bound to metallothionein-like proteins.
abstract: The possibilities of pressurized liquid extraction (PLE) have been novelty tested to assist the cytosol preparation from wet mussel soft tissue before the determination of metals bound to metallothionein-like proteins (MLPs). Results obtained after PLE were compared with those obtained after a classical blending procedure for mussel cytosolic preparation. Isoforms MLP-1 (retention time of 4.1 min) and MLP-2 (retention time of 7.4 min) were separated by anion exchange high-performance liquid chromatography (HPLC) and the concentrations of Ba, Cu, Mn, Sr and Zn bound to MLP isoforms were directly measured by inductively coupled plasma-atomic emission spectrometry (ICP-OES) as a multi-element detector. The optimized PLE-assisted mussel cytosol preparation has consisted of one extraction cycle at room temperature and 1500 psi for 2 min. Since separation between the solid mussel residue and the extract (cytosol) is performed by the PLE system, the cytosol preparation method is faster than conventional cytosol preparation methods by cutting/blending using Ultraturrax or Stomacher devices.
authors.affiliation: Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, University of Santiago de Compostela, Avenida das Ciencias s/n, 15782 Santiago de Compostela, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Santiago-Rivas
authors.forename: Antonio
authors.initials: A
authors.lastname: Moreda-Piñeiro
authors.forename: Pilar
authors.initials: P
authors.lastname: Bermejo-Barrera
authors.forename: Jorge
authors.initials: J
authors.lastname: Moreda-Piñeiro
authors.forename: Elia
authors.initials: E
authors.lastname: Alonso-Rodríguez
authors.forename: Soledad
authors.initials: S
authors.lastname: Muniategui-Lorenzo
authors.forename: Purificación
authors.initials: P
authors.lastname: López-Mahía
authors.forename: Darío
authors.initials: D
authors.lastname: Prada-Rodríguez
mesh_terms: D000818:Animals; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism*; D008668:Metallothionein / Q000378:metabolism*; D019216:Metals, Heavy / Q000032:analysis* / Q000378:metabolism; D049878:Mytilus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011312:Pressure; D011485:Protein Binding; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism
chemical_list: D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; D008668:Metallothionein