title: Serum-derived hepatitis C virus infection of primary human hepatocytes is tetraspanin CD81 dependent.
abstract: Hepatitis C virus-positive serum (HCVser, genotypes 1a to 3a) or HCV cell culture (JFH1/HCVcc) infection of primary normal human hepatocytes was assessed by measuring intracellular HCV RNA strands. Anti-CD81 antibodies and siRNA-CD81 silencing markedly inhibited (>90%) HCVser infection irrespective of HCV genotype, viral load, or liver donor, while hCD81-large intracellular loop (LEL) had no effect. However, JFH1/HCVcc infection of hepatocytes was modestly inhibited (40 to 60%) by both hCD81-LEL and anti-CD81 antibodies. In conclusion, CD81 is involved in HCVser infection of human hepatocytes, and comparative studies of HCVser versus JFH1/HCVcc infection of human hepatocytes and Huh-7.5 cells revealed that the cell-virion combination is determinant of the entry process.
authors.affiliation: Inserm U632, Hepatic Physiopathology, 1919 route de Mende, 34293 Montpellier, Cedex 5, France.
authors.forename: Sonia
authors.initials: S
authors.lastname: Molina
authors.forename: Valerie
authors.initials: V
authors.lastname: Castet
authors.forename: Lydiane
authors.initials: L
authors.lastname: Pichard-Garcia
authors.forename: Czeslaw
authors.initials: C
authors.lastname: Wychowski
authors.forename: Eliane
authors.initials: E
authors.lastname: Meurs
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Pascussi
authors.forename: Camille
authors.initials: C
authors.lastname: Sureau
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Fabre
authors.forename: Antonio
authors.initials: A
authors.lastname: Sacunha
authors.forename: Dominique
authors.initials: D
authors.lastname: Larrey
authors.forename: Jean
authors.initials: J
authors.lastname: Dubuisson
authors.forename: Joliette
authors.initials: J
authors.lastname: Coste
authors.forename: Jane
authors.initials: J
authors.lastname: McKeating
authors.forename: Patrick
authors.initials: P
authors.lastname: Maurel
authors.forename: Chantal
authors.initials: C
authors.lastname: Fournier-Wirth
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D020868:Gene Silencing; D016174:Hepacivirus / Q000502:physiology*; D022781:Hepatocytes / Q000737:chemistry / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis; D011991:Receptors, Virus / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D060225:Tetraspanin 28; D053586:Virus Internalization
chemical_list: D015703:Antigens, CD; C554798:CD81 protein, human; D012367:RNA, Viral; D011991:Receptors, Virus; D060225:Tetraspanin 28