title: The proteasome maturation protein POMP facilitates major steps of 20S proteasome formation at the endoplasmic reticulum.
abstract: The quality control of proteins mediated by the plasticity of the proteasome system is regulated by the timely and flexible formation of this multisubunit proteolytic enzyme complex. Adaptable biogenesis of the 20S proteasome core complex is therefore of vital importance for adjusting to changing proteolytic requirements. However, the molecular mechanism and the cellular sites of mammalian proteasome formation are still unresolved. By using precursor complex-specific antibodies, we now show that the main steps in 20S core complex formation take place at the endoplasmic reticulum (ER). Thereby, the proteasome maturation protein (POMP)--an essential factor of mammalian proteasome biogenesis--interacts with ER membranes, binds to alpha1-7 rings, recruits beta-subunits stepwise and mediates the association of mammalian precursor complexes with the ER. Thus, POMP facilitates the main steps in 20S core complex formation at the ER to coordinate the assembly process and to provide cells with freshly formed proteasomes at their site of function.
authors.affiliation: Institut für Biochemie, Charité-Universitätsmedizin Berlin, Monbijoustrasse 2, 10117 Berlin, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Fricke
authors.forename: Sylvia
authors.initials: S
authors.lastname: Heink
authors.forename: Janos
authors.initials: J
authors.lastname: Steffen
authors.forename: Peter-Michael
authors.initials: PM
authors.lastname: Kloetzel
authors.forename: Elke
authors.initials: E
authors.lastname: Krüger
mesh_terms: D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D047468:Immunoprecipitation; D007425:Intracellular Membranes / Q000378:metabolism; D018613:Microscopy, Confocal; D008954:Models, Biological; D018832:Molecular Chaperones / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D018832:Molecular Chaperones; C110856:proteasome maturation protein; D046988:Proteasome Endopeptidase Complex