title: Decursin and decursinol angelate selectively inhibit NADH-fumarate reductase of Ascaris suum.
abstract: NADH-fumarate reductase (NFRD) is a key enzyme in many anaerobic helminths. Decursin and decursinol angelate have been isolated from the roots of ANGELICA GIGAS Nakai (Apiaceae) as NFRD inhibitors. They inhibited ASCARIS SUUM NFRD with IC (50) values of 1.1 and 2.7 microM, respectively. Their target is the electron transport enzyme complex I. Since the inhibitory activities of decursin against bovine heart complexes are weak, it is a selective inhibitor of the nematode complex I. In contrast, decursinol angelate moderately inhibits bovine heart complexes II and III. Decursinol inhibits A. SUUM NFRD to a similar extent, but its target is complex II. It also inhibits bovine heart complexes II and III.
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan. shiomi@lisci.kitasato-u.ac.jp
authors.forename: Kazuro
authors.initials: K
authors.lastname: Shiomi
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hatano
authors.forename: Hiromi
authors.initials: H
authors.lastname: Morimoto
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ui
authors.forename: Kimitoshi
authors.initials: K
authors.lastname: Sakamoto
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tomoda
authors.forename: Eun Woo
authors.initials: EW
authors.lastname: Lee
authors.forename: Tae Ryeon
authors.initials: TR
authors.lastname: Heo
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kawagishi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Omura
mesh_terms: D029969:Angelica / Q000737:chemistry; D000818:Animals; D000871:Anthelmintics / Q000737:chemistry / Q000494:pharmacology*; D017165:Ascaris suum / Q000201:enzymology*; D001578:Benzopyrans / Q000737:chemistry / Q000494:pharmacology*; D002087:Butyrates / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000037:antagonists & inhibitors*
chemical_list: D000871:Anthelmintics; D001578:Benzopyrans; D002087:Butyrates; C101278:decursin; D044925:Oxidoreductases Acting on CH-CH Group Donors; C020155:fumarate reductase (NADH)