title: [Effect of nuclear factor-kappaB activation on expression of proinflammatory cytokines in rat lung tissues in early stage of burn injury].
abstract: OBJECTIVE
  To investigate the effect of nuclear factor-kappaB (NF-kappaB) activation on the expression of proinflammatory cytokines in the lung tissues of rats with early-stage burn injury.
  
  
  METHODS
  Wistar rats were randomly divided into 3 groups, namely the normal control, burn, burn and PDTC treatment groups, and in the latter two groups, the rats were subjected to 35% TBSA full-thickness burns. Activation of pulmonary NF-kappaB at 1, 3, 6, 12, and 24 postburn hour (PBH) was tested by electrophoretic mobility shift assay , and the expressions of pulmonary tumor necrosis factor alpha (TNF alpha) and interleukin-8 (IL-8) mRNAs at 3, 6, 12, and 24 h were detected by RT-PCR.
  
  
  RESULTS
  Compared to that of the control group, activity of pulmonary NF-kappaB in burned rats was markedly increased within 1 PBH and kept increasing till 24 h. Expressions of pulmonary TNF alpha and IL-8 mRNAs increased gradually, reaching the peak level at 6 PBH, and PDTC could effectively inhibit pulmonary NF-kappaB activation and expression of the pulmonary cytokines induced by the burn injury.
  
  
  CONCLUSIONS
  Severe burn injury may activate pulmonary NF-kappaB, which ultimately leads to secretion of cytokines in the lung tissues.
authors.affiliation: Department of Burns, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Zhi-Qing
authors.initials: ZQ
authors.lastname: Li
authors.forename: Yue-Sheng
authors.initials: YS
authors.lastname: Huang
authors.forename: Zong-Cheng
authors.initials: ZC
authors.lastname: Yang
authors.forename: Jia-Han
authors.initials: JH
authors.lastname: Wang
mesh_terms: D000818:Animals; D002056:Burns / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D015870:Gene Expression; D006801:Humans; D018836:Inflammation Mediators / Q000276:immunology*; D016209:Interleukin-8 / Q000235:genetics* / Q000276:immunology; D008168:Lung / Q000276:immunology* / Q000473:pathology; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000276:immunology
chemical_list: D018836:Inflammation Mediators; D016209:Interleukin-8; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha