[Simultaneous determination of sulfonylurea and diphenylether herbicide residues in soybean and rice by high performance liquid chromatography].
A high performance liquid chromatographic (HPLC) method for the simultaneous determination of sulfonylurea herbicide and diphenylether herbicide residues in soybean and rice samples was developed and evaluated. The analytes in soybean and rice samples were extracted with acetonitrile. The extract was cleaned up using an SPE-C18 cartridge, separated and quantitatively determined by reversed-phase HPLC with an ultraviolet detector. The conditions of the pretreatment for soybean and rice samples as well as the parameters of chromatographic separation were optimized. The method had a good linearity in the range of 0.05-2.0 mg/L. The quantitative limits (LOQ) of the 8 herbicide residues were 0.01-0.02 mg/kg. The spiked recoveries were in the range of 91.6%-116.1% for soybean samples and 76.6%-110.8% for rice samples with the relative standard deviations of 1.0%-12.2%. It is concluded that the proposed method is rapid, accurate and sensitive and can be used for the determination of the eight herbicide residues in soybean and rice.
Wang
Hexing
H
Dept. of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China. wanghexing@tom.com
Li
Yuanqian
Y
Yong
Li
L
Gu
Suying
S
Yang
Xiaoqi
X
Li
Lei
L
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006540:Herbicides / Q000032:analysis* / Q000737:chemistry*; D015394:Molecular Structure; D012275:Oryza / Q000737:chemistry*; D015203:Reproducibility of Results; D013025:Glycine max / Q000737:chemistry*; D013453:Sulfonylurea Compounds / Q000032:analysis* / Q000737:chemistry*
D006540:Herbicides; D013453:Sulfonylurea Compounds