[DNA sequencing of miR-17-92 cluster at chromosome 13q31-q32 in mantel cell lymphoma cell lines].
This study was aimed to explore the characteristics of miR-17-92 cluster at chromosome 13q31-q32 in B cell malignant lymphoma and to investigate the changes of miR-17-92 cluster in B cell lymphoma at genome DNA level and its influence on expression of miR-17-92 cluster and relation with lymphoma occurrence. PCR and DNA sequencing were used to detect miR-17-92 cluster at chromosome 13q31-q32 in mantel cell lymphoma (MCL) cell lines Rec1, G519 and Z138. The results showed that DNA sequence of miR-17-92 cluster at chromosome 13q31-q32 in MCL cell lines were normal. It is concluded that there is no abnormal change in DNA sequence of miR-17-92 cluster which is not the mechanism for miR-17-92 cluster overexpression in mantel cell lymphoma cell lines.
Xu
Wei
W
Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, China.
Li
Jian-Yong
JY
Shen
Qiu-Dan
QD
Li
Li
L
Yu
Hui
H
D001483:Base Sequence; D045744:Cell Line, Tumor; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D006801:Humans; D020522:Lymphoma, Mantle-Cell / Q000235:genetics* / Q000276:immunology; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
C524941:MIRN17 microRNA, human; D035683:MicroRNAs