Metabolic changes in the pig liver during warm ischemia and reperfusion measured by microdialysis.
OBJECTIVE
Portal triad clamping can cause ischemia-reperfusion injury. The aim of the study was to monitor metabolic changes by microdialysis before, during, and after warm ischemia in the pigliver.


METHODS
Eight pigs underwent laparotomy followed by ischemia by Pringle's maneuver. One microdialysis catheter was placed in each of four liver lobes. A reference catheter was placed in a muscle. Microdialysis samples were collected at intervals of 30 min starting 2 h before 1 h of total ischemia followed by 3 h of reperfusion. Glucose, lactate, pyruvate, and glycerol concentrations were measured. Blood samples were drawn for determination of alanine aminotransferase, alkaline phosphatase, and bilirubin together with total leukocytes and prothrombin time.


RESULTS
All parameters were stable during the baseline period. During the ischemic period, lactate levels increased significantly (P < 0.05) followed by a rapid decrease after reperfusion. A transient increase was observed for glucose and glycerol. Pyruvate showed a slight increase from the time of ischemia. The lactate-pyruvate ratio increased rapidly after initiating ischemia and decreased immediately after reperfusion. A slight increase in transaminase levels was observed.


CONCLUSIONS
During and after warm ischemia, there were profound metabolic changes in the pigliver observed with an increase in lactate, glucose, glycerol, and the lactate-pyruvate ratio. There were no differences between the four liver lobes, indicating the piglivers homogeneity.
Kannerup
Anne-Sofie
AS
Department of Surgical Gastroenterology L, Aarhus University Hospital, Aarhus C, Denmark. askannerup@gmail.com
Funch-Jensen
Peter
P
Grønbaek
Henning
H
Jørgensen
Rasmus Langelund
RL
Mortensen
Frank Viborg
FV
D000818:Animals; D005260:Female; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007511:Ischemia / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism; D008099:Liver / Q000098:blood supply* / Q000378:metabolism*; D017551:Microdialysis; D011773:Pyruvates / Q000378:metabolism; D015424:Reperfusion; D013552:Swine
D011773:Pyruvates; D019344:Lactic Acid; D005947:Glucose; D005990:Glycerol