title: dTrf2 is required for transcriptional and developmental responses to ecdysone during Drosophila metamorphosis.
abstract: The TATA box-binding protein (TBP) related factor 2 (TRF2) has been well characterized at a biochemical level and in cultured cells. Relatively little, however, is known about how TRF2 functions in specific biological pathways during development. Here, we show that Drosophila TRF2 (dTRF2) plays an essential role in responses to the steroid hormone ecdysone during the onset of metamorphosis. Hypomorphic dTrf2 mutations lead to developmental arrest during prepupal and early pupal stages with defects in major ecdysone-triggered biological responses, including puparium formation, anterior spiracle eversion, gas bubble translocation, adult head eversion, and larval salivary gland cell death. The transcription of key ecdysone-regulated target genes is delayed and reduced in dTrf2 mutants. dTrf2 appears to be required for the proper timing and levels of ecdysone-regulated gene expression required for entry into metamorphosis.
authors.affiliation: Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, Utah 84112-5330, USA.
authors.forename: Arash
authors.initials: A
authors.lastname: Bashirullah
authors.forename: Geanette
authors.initials: G
authors.lastname: Lam
authors.forename: Viravuth P
authors.initials: VP
authors.lastname: Yin
authors.forename: Carl S
authors.initials: CS
authors.lastname: Thummel
mesh_terms: D000483:Alleles; D000818:Animals; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D004440:Ecdysone / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D008675:Metamorphosis, Biological / Q000235:genetics*; D009154:Mutation; D035341:Telomeric Repeat Binding Protein 2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D035341:Telomeric Repeat Binding Protein 2; D004440:Ecdysone