SNPbox: web-based high-throughput primer design with an eye for repetitive sequences.
In this omics era, researchers in biosciences need more than ever user-friendly, fast, and straightforward computational tools for high-throughput research. In this chapter, we present SNPbox and explain the general primer design strategy. We also show how the four modules, Exon, Single-Nucleotide Polymorphism (SNP), Saturation, and Exon + Saturation, exactly apply the primer design strategy, give clear guidance for users of the Web interface at http://www.SNPbox.org, and explain how pre-designed primers for Ensembl genes can be visualized and retrieved.
Weckx
Stefan
S
Department of Molecular Genetics, Flanders Interuniversity Institute for Biotechnology (VIB), University of Antwerp, Belgium.
De Rijk
Peter
P
Glassee
Wim
W
Van Broeckhoven
Christine
C
Del-Favero
Jurgen
J
D017931:DNA Primers / Q000737:chemistry; D005091:Exons; D020407:Internet; D020641:Polymorphism, Single Nucleotide; D012091:Repetitive Sequences, Nucleic Acid; D014584:User-Computer Interface
D017931:DNA Primers