title: The kinetics of Ca(2+)-dependent switching in a calmodulin-IQ domain complex.
abstract: We have performed a kinetic analysis of Ca2+-dependent switching in the complex between calmodulin (CaM) and the IQ domain from neuromodulin, and have developed detailed kinetic models for this process. Our results indicate that the affinity of the C-ter Ca2+-binding sites in bound CaM is reduced due to a approximately 10-fold decrease in the Ca2+ association rate, while the affinity of the N-ter Ca2+-binding sites is increased due to a approximately 3-fold decrease in the Ca2+ dissociation rate. Although the Ca2+-free and Ca2+-saturated forms of the CaM-IQ domain complex have identical affinities, CaM dissociates approximately 100 times faster in the presence of Ca2+. Furthermore, under these conditions CaM can be transferred to the CaM-binding domain from CaM kinase II via a ternary complex. These properties are consistent with the hypothesis that CaM bound to neuromodulin comprises a localized store that can be efficiently delivered to neuronal proteins in its Ca2+-bound form in response to a Ca2+ signal.
authors.affiliation: Division of Molecular Biology and Biochemistry, University of Missouri-Kansas City, Kansas City, Missouri 64110-2499, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Black
authors.forename: J Eva
authors.initials: JE
authors.lastname: Selfridge
authors.forename: Anthony
authors.initials: A
authors.lastname: Persechini
mesh_terms: D001665:Binding Sites; D002118:Calcium / Q000737:chemistry* / Q000378:metabolism; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000737:chemistry / Q000378:metabolism; D002147:Calmodulin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D011487:Protein Conformation
chemical_list: C512165:BSCaMIQ peptide; D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D002118:Calcium