title: Analysis of TIMP expression and activity.
abstract: This chapter provides practical information on the assay of tissue inhibitor of metalloproteinase (TIMP) activity and background information enabling meaningful interpretation of the data. Protocols are given for assessing the presence of TIMPs in biological samples by immunoblotting and by virtue of their ability to inhibit matrix metalloproteinase (MMP) hydrolysis of protein substrates (reverse zymography) and synthetic fluorogenic substrates.
authors.forename: Linda
authors.initials: L
authors.lastname: Troeberg
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nagase
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005456:Fluorescent Dyes; D005780:Gelatin; D006801:Humans; D007700:Kinetics; D061965:Matrix Metalloproteinase Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D061965:Matrix Metalloproteinase Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases; D005780:Gelatin