title: Time fluctuations and imaging in the SERS spectra of fungal hypha grown on nanostructured substrates.
abstract: Surface-enhanced Raman scattering (SERS) spectroscopy is an emerging technique in biomolecular analysis that can have a tremendous impact in the life sciences. We report on the SERS imaging of fungal hyphae grown on nanostructured SERS active substrates engineered using semiconductor technologies. Time fluctuations in the intensity and band position in the SERS spectra measured on the same sample position with 1 s integration time have been observed indicating that the SERS signal arises from a limited number of molecules and that possibly single components are being detected.
authors.affiliation: Department of Chemistry, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
authors.forename: Adriana
authors.initials: A
authors.lastname: Szeghalmi
authors.forename: Susan
authors.initials: S
authors.lastname: Kaminskyj
authors.forename: Petra
authors.initials: P
authors.lastname: Rösch
authors.forename: Jürgen
authors.initials: J
authors.lastname: Popp
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Gough
mesh_terms: D000362:Agar / Q000737:chemistry; D001233:Aspergillus nidulans / Q000737:chemistry* / Q000254:growth & development*; D006046:Gold / Q000737:chemistry*; D025301:Hyphae / Q000737:chemistry / Q000254:growth & development; D049329:Nanostructures / Q000737:chemistry*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D006046:Gold; D000362:Agar