Increased amount of Bifidobacterium thermacidophilum and Megasphaera elsdenii in the colonic microbiota of pigs fed a swine dysentery preventive diet containing chicory roots and sweet lupine.
OBJECTIVE
To investigate which specific bacterial species that were stimulated or inhibited in the proximal colon of pigs when a fructan-rich diet was compared with a diet that contained resistant carbohydrates. The study focussed especially on Bifidobacterial species by using a noncultureable approach.


RESULTS
Terminal restriction fragment length polymorphism (T-RFLP) was used to describe differences in the total colonic microbiota as well as in the populations of Bifidobacterium spp. in pigs fed with a fructan-rich diet and a diet containing resistant carbohydrates. The fructan-rich diet has previously been shown to prevent swine dysentery caused by Brachyspira hyodysenteriae. The T-RFLP profiling, 16S rRNA gene cloning and in situ hybridization showed that the pigs fed with the fructan-rich diet had a higher proportion of Bifidobacterium thermacidophilum subsp. porcinum and Megasphaera elsdenii.


CONCLUSIONS
These findings suggested that the bacterial fructan fermentation occurring in the porcine colon might be cross-feeding of lactate produced by B. thermacidophilum and used by M. elsdenii.


CONCLUSIONS
B. thermacidophilum and M. elsdenii may be the course of the inhibition of the pathogenic bacteria Brach. hyodysenteriae in colon of pigs when they are fed fructan-rich diets.
Mølbak
L
L
National Veterinary Institute, Technical University of Denmark, Copenhagen, Denmark. lam@vet.dtu.dk
Thomsen
L E
LE
Jensen
T K
TK
Bach Knudsen
K E
KE
Boye
M
M
D000821:Animal Feed; D000818:Animals; D001644:Bifidobacterium / Q000302:isolation & purification* / Q000378:metabolism; D017401:Brachyspira hyodysenteriae / Q000378:metabolism / Q000502:physiology; D018651:Cichorium intybus; D003106:Colon; D015169:Colony Count, Microbial; D005630:Fructans / Q000008:administration & dosage; D005766:Gastrointestinal Contents / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000517:prevention & control*; D007773:Lactates / Q000378:metabolism; D031297:Lupinus; D045851:Megasphaera / Q000302:isolation & purification* / Q000378:metabolism; D013552:Swine / Q000382:microbiology*; D013553:Swine Diseases / Q000378:metabolism / Q000382:microbiology / Q000517:prevention & control*
D005630:Fructans; D007773:Lactates