Changtai granule, a traditional Chinese drug, protects hapten-induced colitis by attenuating inflammatory and immune dysfunctions.
The study was aimed to investigate the effects and mechanism of action of Changtai granule (CT), a traditional compound Chinese medicinal formula, in rodent 2,4,6-trinitrobenzene sulfonic acid (TNBS) colitis. Rats with TNBS/ethanol-induced colitis were used. The colonic wet weight, myeloperoxidase (MPO) activity, macroscopic and histological colon injury was observed. Inflammation cytokines were determined by ELISA methods and semi-quantitative RT-PCR. When dosed orally once daily, CT markedly attenuated TNBS-induced colitis. CT significantly attenuated colonic wet weight, macroscopic and histological colon injury. CT decreased mucosal mRNA levels for several inflammatory mediators: inducible nitric oxide synthase, cyclooxygenase 2, and macrophage inflammatory protein 2. CT also decreased mucosal mRNA and protein levels of T effectors cytokines: tumor necrosis factor-alpha (TNF-alpha), interleukin-2 (IL-2) and interferon-gamma (IFN-gamma). Systemic levels of these cytokines were also dramatically attenuated. CD3/CD28-mediated costimulation of T helper 1 effector cytokines release in lamina propria mononuclear cells (LPMC) was markedly inhibited by CT ex vivo and in vitro. Also CT prevented cytokines production by nuclear factor-kappaB (NF-kappaB). The potential anti-inflammatory and immunomodulatory effect of CT in TNBS colitis suggests that CT may be an effective treatment approach for inflammatory bowel disease.
Liu
Bin-Guo
BG
254th Hospital of Peoples Liberation Army, Huangwei Road, Tianjin 300142, PR China.
Jia
Xin-Ming
XM
Cao
Ying-Ying
YY
Chen
Shang-Hong
SH
Gao
Ping-Hui
PH
Wang
Yan
Y
Jiang
Yuan-Ying
YY
Cao
Yong-Bin
YB
D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D028481:Euphorbia / Q000737:chemistry; D006241:Haptens; D007249:Inflammation / Q000188:drug therapy* / Q000209:etiology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008297:Male; D008516:Medicine, Chinese Traditional; D032102:Phellodendron / Q000737:chemistry; D028321:Plant Preparations / Q000494:pharmacology*; D028502:Polygonum / Q000737:chemistry; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D031993:Sanguisorba / Q000737:chemistry; D014302:Trinitrobenzenesulfonic Acid
D006241:Haptens; D028321:Plant Preparations; D012333:RNA, Messenger; D014302:Trinitrobenzenesulfonic Acid