An efficient process for production and purification of hyaluronic acid from Streptococcus equi subsp. zooepidemicus.
Growth of Streptococcus zooepidemicus in a 10 l bioreactor with 50 g sucrose/l and 10 g casein hydrolysate/l gave 5-6 g hyaluronic acid/l after 24-28 h. Purification of hyaluronic acid gave a recovery of 65% with the final material having an Mr of approximately 4 x 10(6) Da with less than 0.1% protein.
Rangaswamy
Vidhya
V
Industrial Biotechnology Group, Reliance Life Sciences Pvt. Ltd, R-282, TTC area of MIDC, Thane-Belapur Road, Rabale, Navi Mumbai, India. vidhya_rangaswamy@relbio.com
Jain
Dharmendra
D
D019149:Bioreactors; D006820:Hyaluronic Acid / Q000096:biosynthesis* / Q000302:isolation & purification*; D007218:Industrial Microbiology / Q000379:methods*; D018502:Streptococcus equi / Q000254:growth & development / Q000378:metabolism*
D006820:Hyaluronic Acid