title: Three-dimensional digital image analysis of immunostained neurons in thick tissue sections.
abstract: Detection and three dimensional reconstruction of cell structures from brightfield microscopy video clips using digital image processing algorithms is presented. While the confocal microscopy offers an efficient technique for three dimensional measurements, extensive and repeated measurements are still often better to be performed using permanent staining and brightfield microscopy. By processing of brightfield microscopy videos using automated and efficient digital image processing algorithms, the tedious task of manual analysis can be avoided. Our two-stage algorithm is applied for 1) cell soma detection and 2) identification of the 3D structure of entire neurons. To verify the results, we present 3D reconstructions of the detected cells.
authors.affiliation: Inst. of Signal Process., Tampere Univ. of Technol., Finland. jyrki.selinummi@tut.fi
authors.forename: Jyrki
authors.initials: J
authors.lastname: Selinummi
authors.forename: Pekka
authors.initials: P
authors.lastname: Ruusuvuori
authors.forename: Antti
authors.initials: A
authors.lastname: Lehmussola
authors.forename: Heikki
authors.initials: H
authors.lastname: Huttunen
authors.forename: Olli
authors.initials: O
authors.lastname: Yli-Harja
authors.forename: Riitta
authors.initials: R
authors.lastname: Miettinen
mesh_terms: D000465:Algorithms; D000818:Animals; D001331:Automation; D064032:Calbindin 2; D003712:Dendrites / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods; D018715:Microscopy, Video / Q000295:instrumentation* / Q000379:methods; D009474:Neurons / Q000473:pathology*; D010363:Pattern Recognition, Automated; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D064030:S100 Calcium Binding Protein G / Q000737:chemistry
chemical_list: D064032:Calbindin 2; D064030:S100 Calcium Binding Protein G