title: Modified oligonucleotides as tools for allele-specific amplification.
abstract: Allele-specific polymerase chain reaction (PCR), a method that reports nucleotide variations through either the presence or the absence of a DNA product obtained through PCR amplification, holds the promise to combine target amplification and analysis in one single step. Recently, it has been reported that the selectivity of allele-specific PCR can be significantly increased through the employment of chemically modified primer probes. Here, we report on significant developments of primer probe design and synthesis along this line.
authors.affiliation: Konstanz University, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Strerath
authors.forename: Ilka
authors.initials: I
authors.lastname: Detmer
authors.forename: Jens
authors.initials: J
authors.lastname: Gaster
authors.forename: Andreas
authors.initials: A
authors.lastname: Marx
mesh_terms: D000483:Alleles; D017931:DNA Primers / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers