title: A high-throughput soft agar assay for identification of anticancer compound.
abstract: A 384-well soft agar assay was developed to identify potential novel anticancer compounds. Normally used to detect cell transformation, the assay is used here to quantitate cell proliferation in a 3-dimensional (3-D) anchorage-independent format. HCC827 cells, which are highly sensitive to epithelial growth factor receptor (EGFR) tyrosine kinase inhibitors, were used to develop the method and a set of 9600 compounds used to validate the assay. Results were compared to a monolayer assay using the same compound set. The assay provides a robust method to discover compounds that could be missed using traditional monolayer formats.
authors.affiliation: Abbott Laboratories, Global Pharmaceutical Research and Development, Department of Biological Screening, Abbott Park, IL, USA.
authors.forename: Steven N
authors.initials: SN
authors.lastname: Anderson
authors.forename: Danli L
authors.initials: DL
authors.lastname: Towne
authors.forename: David J
authors.initials: DJ
authors.lastname: Burns
authors.forename: Usha
authors.initials: U
authors.lastname: Warrior
mesh_terms: D000362:Agar; D000970:Antineoplastic Agents / Q000737:chemistry*; D045744:Cell Line, Tumor; D004791:Enzyme Inhibitors / Q000737:chemistry*; D066246:ErbB Receptors / Q000037:antagonists & inhibitors; D006801:Humans
chemical_list: D000970:Antineoplastic Agents; D004791:Enzyme Inhibitors; D000362:Agar; D066246:ErbB Receptors