Simultaneous extraction of epimedin A, B, C and icariin from Herba Epimedii by ultrasonic technique.
An ultrasonic-assisted extraction (UAE) procedure of epimedin A, epimedin B, epimedin C and icariin from Herba Epimedii was developed. The effects of ethanol concentration, ratio of liquid to solid, UAE time, extraction temperature and number of extraction cycles on the extraction yields of the four flavonoids from Herba Epimedii were investigated. The optimal UAE condition was found using orthogonal test: 50% (v/v) ethanol solution, liquid:solid ratio of 30 ml/g, ultrasonication duration 30 min, extraction temperature 50 degrees C and three extraction cycles. The UAE method showed a high reproducibility. Epimedin A, B, C and icariin in the crude extract exhibited photodegradation under ultraviolet irradiation. This UAE method was shown to be highly efficient compared with the conventional Soxhlet extraction and boiling extraction. The effect of ultrasound on cell destruction was examined by scanning electron microscopy. The contents of epimedin A, B, C and icariin in the leaves of 20 Epimedium species were determined using high-performance liquid chromatographic method following UAE method.
Zhang
Hua-Feng
HF
Key Laboratory of Plant Conservation Genetics, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, PR China; Graduate University of Chinese Academy of Sciences, Beijing 100049, PR China.
Yang
Tian-Shun
TS
Key Laboratory of Plant Conservation Genetics, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, PR China.
Li
Zuo-Zhou
ZZ
Key Laboratory of Plant Conservation Genetics, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, PR China.
Wang
Ying
Y
Key Laboratory of Plant Conservation Genetics, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, PR China. Electronic address: yingwang@wbgcas.cn.
D002851:Chromatography, High Pressure Liquid; D034181:Epimedium / Q000737:chemistry* / Q000528:radiation effects*; D005419:Flavonoids / Q000302:isolation & purification*; D007202:Indicators and Reagents; D008855:Microscopy, Electron, Scanning; D010777:Photochemistry; D010936:Plant Extracts / Q000737:chemistry / Q000528:radiation effects; D015203:Reproducibility of Results; D014465:Ultrasonics
D005419:Flavonoids; D007202:Indicators and Reagents; D010936:Plant Extracts; C529106:epimedin A; C106053:epimedin B; C106054:epimedin C; C056599:icariin