[Influence of daidzein on left ventricular remodeling in rats with hypertrophic myocardium induced by pressure overload].
OBJECTIVE
To investigate the protective effects of daidzein (DD) on ventricular remodeling in rats with myocardial hypertrophy induced by pressure overload and its mechanism.


METHODS
Myocardial hypertrophy model of rats induced by pressure overload was prepared by constricting abdominal aorta. The operated rats were randomly divided into sham operated control group, aorta-constricted model group and three DD groups (30, 60, 120 mg kg(-1)). Four weeks later, the heart-weight (HW), left ventricular weight (LVW), the ratio of HW/BW and LVW/BW (LVI) and the cardio-myocyte diameters (MD) after dyeing by HE colar were measured. The hydroxyroline, nitric oxide (NO) and the activity of nitric oxide synthetase (NOS) and Na+ -K+ -ATPase, Ca2+ -ATPase in left ventricle were quantified with spectrophotometry and the angiotension II (Ang II) in left ventricle and serum was messured with radioimmunoassay.


RESULTS
After treatment of the left ventricular with DD, vs aorta-contricted model group, NO content, cNOS and Na+ -K+ -ATPase, Ca2+ -ATPase activity were significantly increased, the content of AngII in left ventricle and serum and iNOS activity and the ratio of HW/BW, LVI, MD were significantly reduced.


CONCLUSIONS
DD has protective effects on ventricular remodeling in rats with myocardial hypertrophy induced by pressure overload and its mechanism may be related to raising NO content and reducing the level of Ang II.
Zhou
Li
L
Gannan Medical College, Ganzhou 341000, China. zhouli6099@yahoo.com.cn
Zhou
Qing
Q
Xiong
Xiao-qing
XQ
Liu
Jian-xin
JX
He
Wei
W
D000804:Angiotensin II / Q000097:blood / Q000378:metabolism; D000818:Animals; D017379:Hypertrophy, Left Ventricular / Q000378:metabolism / Q000473:pathology*; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D009569:Nitric Oxide / Q000378:metabolism; D048789:Phytoestrogens / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020257:Ventricular Remodeling / Q000187:drug effects*
D007529:Isoflavones; D048789:Phytoestrogens; D000804:Angiotensin II; D009569:Nitric Oxide; C004742:daidzein