title: beta-TCP microporosity decreases the viability and osteoblast differentiation of human bone marrow stromal cells.
abstract: Association of osteoprogenitor cells to calcium phosphate ceramics is currently under intense investigation, for its considered ability to induce bone formation and therefore to allow the successful repair of large bone defects. However, if the first experimental and clinical studies provided promising results, lack of new bone formation has been reported in a large number of animal experiments. In this context and since it has been reported that in some conditions, calcium phosphate ceramic microstructure induces ectopic bone formation, we investigated the effects of ceramic microporosity on the behavior of osteoprogenitor cells for the development of hybrid materials. Human bone marrow stromal cells (BMSCs) were seeded on beta-tricalcium phosphate (TCP) ceramics with 0, 25, or 45% microporosity and cell adhesion, viability, and osteoblastic differentiation have been studied for three weeks. Cell counts, measurement of MTS conversion, and LDH activity indicated that microporosity decreased the viability of BMSCs in a time and rate-dependent manner. In addition, microporosity inhibited osteoblastic differentiation as compared with nonmicroporous ceramics, as revealed by decreased alkaline phosphatase activity and osteocalcin secretion. Results of this in vitro study therefore highlight a negative role for beta-TCP microporosity in the behavior of human osteoprogenitor cells.
authors.affiliation: Laboratory of Cellular and Molecular Biology EA2306, ULCO, Quai Masset Bassin, Napoléon Boulogne, mer France.
authors.forename: Juliane
authors.initials: J
authors.lastname: Isaac
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Hornez
authors.forename: Ding
authors.initials: D
authors.lastname: Jian
authors.forename: Michel
authors.initials: M
authors.lastname: Descamps
authors.forename: Pierre
authors.initials: P
authors.lastname: Hardouin
authors.forename: David
authors.initials: D
authors.lastname: Magne
mesh_terms: D001854:Bone Marrow Cells; D002130:Calcium Phosphates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis; D016062:Porosity; D017154:Stromal Cells / Q000166:cytology* / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods
chemical_list: D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate