[Expressions of micro-calpain mRNA and protein in cerebral tissues of neonatal rats after hypoxic-ischemic brain damage].
OBJECTIVE
To identify the expression of micro-calpain mRNA and protein in cerebral tissues of neonatal rats after hypoxic-ischemic brain damage (HIBD) and its role in the pathogenesis of HIBD.


METHODS
Seven-day-old Sprague-Dawley rats were randomly divided into seven groups: control group and groups with 0, 6, 12, 24, 48, and 72 hours of exposure to HIDB. Quantitative real-time fluroscent polymerase chain reaction and Western blot technique were employed to detect the expression of micro-calpain mRNA and its protein respectively.


RESULTS
The micro-calpain mRNA in the ipsilateral cerebral tissues began to increase 6 hours after the HIDB and reached peak at 24 hours, and then gradually decreased but remained in a higher level than that of the control group until at 72 hours (P < 0.05). The activated micro-calpain protein started to increase 6-12 hours after the HIDB (P < 0.05) and reached peak at 24 hours (P < 0.01). Higher levels of micro-calpain protein were maintained until 48 and 72 hours after the HIBD (P < 0.05).


CONCLUSIONS
The expressions of micro-calpain mRNA and protein increase with the HIBD. The micro-calpain may play a role in the pathogenesis of the HIBD.
Chen
Li-na
LN
Depratment of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Yao
Yu-jia
YJ
Chen
Da-peng
DP
Chen
Yong-xiu
YX
Feng
Xue
X
D000818:Animals; D002154:Calpain / Q000378:metabolism*; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; D002154:Calpain