title: A comparative study of the cellular immune response in patients with stage IB cervical squamous cell carcinoma. Low numbers of several immune cell subtypes are strongly associated with relapse of disease within 5 years.
abstract: OBJECTIVE
  The purpose of this study was to investigate possible differences in the primary in situ cellular immune response between patients with and without relapse of Stage IB cervical squamous cell carcinoma.
  
  
  METHODS
  Paraffin-embedded tissue from 40 patients (20 with and 20 without relapse) was evaluated. Sections were immunostained for CD1a+, CD3+, CD4+, CD8+, CD20+, CD45RA+, CD45RO+, CD57+, CD68+ and GrB+ cells. Immune cell profile densities were estimated using stereology.
  
  
  RESULTS
  We found significantly lower densities of CD3+, CD4+, CD8+ and CD57+ cells (both intra- and peritumoral) in tissue from patients who had relapse. Also densities of intratumoral CD1a+ cells and peritumoral CD20+, CD45RA+ and CD45RO+ cells were significantly lower among patients with relapse.
  
  
  CONCLUSIONS
  This study demonstrates striking differences in the cellular immune response between patients with and without relapse within 5 years. The results are of potential value in adjuvant immunotherapy and prediction of prognosis.
authors.affiliation: Department of Oncology, Aalborg Hospital, Aarhus University Hospital, Denmark. b.nedergaard@rn.dk
authors.forename: Bettina S
authors.initials: BS
authors.lastname: Nedergaard
authors.forename: Morten
authors.initials: M
authors.lastname: Ladekarl
authors.forename: Jens Randel
authors.initials: JR
authors.lastname: Nyengaard
authors.forename: Karsten
authors.initials: K
authors.lastname: Nielsen
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000473:pathology / Q000532:radiotherapy / Q000601:surgery; D005260:Female; D006801:Humans; D007044:Hysterectomy; D007111:Immunity, Cellular / Q000276:immunology; D007150:Immunohistochemistry; D008197:Lymph Node Excision; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D008875:Middle Aged; D009367:Neoplasm Staging; D012008:Recurrence; D002583:Uterine Cervical Neoplasms / Q000276:immunology* / Q000473:pathology / Q000532:radiotherapy / Q000601:surgery
chemical_list: D015703:Antigens, CD