[Optimization of manninotriose preparation from stachoyse].
OBJECTIVE
To optimize the processing of mannatriose preparation from stachyose by acidolysis.


METHODS
An orthogonal experiment was performed to select a better condition of acidolysis. Fructoes and stachyose was determined by HPLC.


RESULTS
Among the factors set in the present work, temperature and time have little influence on the acidolysis, while the effect of pH value was significant (P < 0.05). The best processing was selected as heating stachosy solution for 12h at pH 2.5 and 90 degrees C.


CONCLUSIONS
It is practicable to prepare manninotriose from stachyoses by acidolysis.
Wu
Wei-hong
WH
School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China. weihong204@163.com
Wen
Xue-sen
XS
Zhao
Yu
Y
D000143:Acids / Q000378:metabolism; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009844:Oligosaccharides / Q000737:chemistry*; D032267:Rehmannia / Q000737:chemistry*; D012996:Solutions; D013696:Temperature; D013997:Time Factors; D014312:Trisaccharides / Q000302:isolation & purification*
D000143:Acids; D009844:Oligosaccharides; D012996:Solutions; D014312:Trisaccharides; C069155:manninotriose; C005695:stachyose