title: Introduction of the foreign transposon Tn4560 in Streptomyces coelicolor leads to genetic instability near the native insertion sequence IS1649.
abstract: We report an altered pattern of genetic instability for Streptomyces coelicolor when the bacterium harbored a foreign transposon, Tn4560. Deletions, amplifications, and circularizations of the linear 8.7-Mb chromosome occurred more frequently at sites adjacent to native insertion elements, notably IS1649. In contrast, deletions, amplifications, and circularizations of a wild-type strain happened at heterogeneous sites within the chromosome. In 50 strains examined, structural changes removed or duplicated hundreds of contiguous S. coelicolor genes, altering up to 33% of the chromosome. S. coelicolor shows a bias toward one type of genetic instability during this particular assault from the environment, the invasion of foreign DNA.
authors.affiliation: Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA. ewidenbrant@gmail.com
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Widenbrant
authors.forename: Camilla M
authors.initials: CM
authors.lastname: Kao
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D042822:Genomic Instability; D011995:Recombination, Genetic; D017384:Sequence Deletion; D048372:Streptomyces coelicolor / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial