Protein targeting to mitochondria of Saccharomyces cerevisiae and Neurospora crassa: in vitro and in vivo studies.
Most studies on the biogenesis of mitochondrial proteins have been carried out using fungal mitochondria as a model system. In particular, baker's yeast, Saccharomyces cerevisiae, combines several experimental advantages, allowing both genetic and biochemical approaches and thus a combination of investigations in vivo and in vitro. However, the red bread mold Neurospora crassa has also been an important research tool. Isolated mitochondria can be used from both organisms for import experiments in a reconstituted system, using radiolabeled precursor proteins synthesized in reticulocyte lysate or purified preproteins. Assays are available for studies on the import pathways and localization of mitochondrial proteins and for the characterization of the components of the protein import machinery.
Papatheodorou
Panagiotis
P
Institut für Physiologische Chemie, Ruhr-Universität Bochum, Bochum, Germany.
Domanska
Grazyna
G
Rassow
Joachim
J
D001671:Biochemistry / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D066298:In Vitro Techniques; D008928:Mitochondria / Q000378:metabolism*; D009492:Neurospora crassa / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D011498:Protein Precursors; D049452:Green Fluorescent Proteins