Bioluminescence imaging of calvarial bone repair using bone marrow and adipose tissue-derived mesenchymal stem cells.
A combined strategy using bioluminescence imaging, bone densitometry and histology was used to analyze the bone regeneration capacity of human bone marrow (hBMSC) and adipose tissue (hAMSC) mesenchymal stem cells, seeded in an osteoconductive arginine-glycine-aspartate (RGD) crosslinked hydrogel scaffold, implanted in a mouse calvarial bone defect. We show that firefly luciferase labeled stem cells can be monitored in vivo through a prolonged 90 days period, during which hBMSCs survive better than hAMSCs and that the density of scaffold bearing defects increased significantly more than that of defects without scaffolds.
Dégano
Irene R
IR
Cardiovascular Research Center (CSIC-ICCC), Hospital de Sant Pau, Barcelona 08025, Spain.
Vilalta
Marta
M
Bagó
Juli R
JR
Matthies
Annette M
AM
Hubbell
Jeffrey A
JA
Dimitriou
Helen
H
Bianco
Paolo
P
Rubio
Nuria
N
Blanco
Jerónimo
J
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D015870:Gene Expression; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D008163:Luminescent Measurements; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010012:Osteogenesis; D012038:Regeneration; D012886:Skull / Q000002:abnormalities / Q000166:cytology* / Q000502:physiology*