Simultaneous determination of malachite green, gentian violet and their leuco-metabolites in aquatic products by high-performance liquid chromatography-linear ion trap mass spectrometry.
A method has been developed for the simultaneous determination of malachite green, gentian violet and their leuco-metabolites in various aquatic products using isotope dilution liquid chromatography-linear ion trap mass spectrometry without post-column oxidation. Sample was extracted with McIlvaine buffer and acetonitrile, followed by partitioning with dichloromethane, purified on basic alumina and OASIS MCX SPE column, and finally analyzed by LC-ESI-MS/MS with the select reaction monitoring (SRM) mode. Decision limits (CCalpha, alpha=0.01) and detection capability (CCbeta, beta=0.05) of the method were in the range of 0.02-0.09 and 0.04-0.13microg/kg for MG, GV, LMG and LGV in grass carp, eel, salmon, shrimp and shellfish, respectively, recoveries of MG, GV, LMG and LGV at all fortification levels (0.25-10microg/kg) were from 80.8% to 115.7%, inter-day relative standard derivations were from 1.9% to 18.4%. This method appeared suitable for the control of MG, GV, LMG and LGV residues in aquatic products.
Wu
Xueli
X
National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Zhang
Gong
G
Wu
Yongning
Y
Hou
Xiaolin
X
Yuan
Zonghui
Z
D000814:Aniline Compounds / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004396:Coloring Agents / Q000032:analysis*; D004524:Eels; D005396:Fish Products / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D005659:Fungicides, Industrial / Q000032:analysis*; D005840:Gentian Violet / Q000032:analysis* / Q000378:metabolism; D009132:Muscles / Q000737:chemistry / Q000378:metabolism; D015203:Reproducibility of Results; D012394:Rosaniline Dyes / Q000032:analysis* / Q000378:metabolism; D012474:Salmon; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D014337:Trout
D000814:Aniline Compounds; D004396:Coloring Agents; D005659:Fungicides, Industrial; D012394:Rosaniline Dyes; C005095:malachite green; C041914:leucogentian violet; C015320:leucomalachite green; D005840:Gentian Violet