A 2,5-dihydroxybenzoic acid/N,N-dimethylaniline matrix for the analysis of oligosaccharides by matrix-assisted laser desorption/ionization mass spectrometry.
The use of a novel 2,5-dihydroxybenzoic acid/N,N-dimethylaniline (DHB/DMA) matrix-assisted laser desorption/ionization (MALDI) matrix for detection and quantitative analysis of native N-linked oligosaccharides was investigated in this study. Substantial improvements in sensitivity were observed relative to the signals obtained with a traditional DHB matrix. Moreover, the morphology of the matrix crystal layer was very uniform, unlike that of DHB. This resulted in highly homogeneous sample distribution throughout the spot, allowing reproducible and consistent mass spectra to be obtained without spot-to-spot variations in signal. Here, we also demonstrate an approach for performing sensitive and accurate quantitative analysis of native N-linked glycans with this novel matrix using an internal standard method.
Snovida
Sergei I
SI
Department of Chemistry, University of Manitoba, Winnipeg, Manitoba, Canada.
Perreault
Hélène
H
D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D002645:Chickens; D005841:Gentisates / Q000737:chemistry*; D009844:Oligosaccharides / Q000032:analysis*; D010047:Ovalbumin / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods*
D000814:Aniline Compounds; D005841:Gentisates; D009844:Oligosaccharides; D011134:Polysaccharides; C015157:N,N-dimethylaniline; D010047:Ovalbumin; C010925:2,5-dihydroxybenzoic acid