title: [The role of transcription factors in the response of mouse lymphocytes to low-level electromagnetic and laser radiations].
abstract: The effects of low-intensity laser radiation (LILR, 632.8 nm, 0.2 mW/cm2) and low-intensity electromagnetic waves (LIEW, 8.15 - 18 GHz, 1 MW/cm2) on the production of transcription factors in lymphocytes from NMRI male mice were examined. The total level of NF-KB and its phosphorylated metabolite Phospho-NF-kappaB, as well as the regulatory protein IkappaB-alpha were determined in spleen lymphocytes subjected to laser or microwave radiations. The proteins were determined by immunoblotting. Laser light induced a lowering in the level of NF-kappaB and IkappaB-alpha. By contrast, irradiation with electromagnetic waves resulted in a significant increase in the amount of NF-kappaB and IkappaB-alpha. The phosphorylated form of NF-kappaB did not noticeably change under either of the two kinds of radiation. The results showed that electromagnetic waves activate the production of both NF-kappaB and the regulatory protein IkappaB-alpha and these data confirm the stress character of the response of spleen lymphocytes to low-level microwaves of the centimeter range.
authors.forename: M O
authors.initials: MO
authors.lastname: Khrenov
authors.forename: D A
authors.initials: DA
authors.lastname: Cherenkov
authors.forename: O V
authors.initials: OV
authors.lastname: Glushkova
authors.forename: T V
authors.initials: TV
authors.lastname: Novoselova
authors.forename: S M
authors.initials: SM
authors.lastname: Lunin
authors.forename: S B
authors.initials: SB
authors.lastname: Parfeniuk
authors.forename: E A
authors.initials: EA
authors.lastname: Lysenko
authors.forename: E G
authors.initials: EG
authors.lastname: Novoselova
authors.forename: E E
authors.initials: EE
authors.lastname: Fesenko
mesh_terms: D000818:Animals; D020672:I-kappa B Proteins / Q000378:metabolism*; D007834:Lasers; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008872:Microwaves; D016328:NF-kappa B / Q000378:metabolism*; D010766:Phosphorylation / Q000528:radiation effects; D011499:Protein Processing, Post-Translational / Q000528:radiation effects*; D013154:Spleen / Q000166:cytology / Q000378:metabolism*
chemical_list: D020672:I-kappa B Proteins; D016328:NF-kappa B