[Identification and molecular study on vibrio cholerae in sea products].
OBJECTIVE
To investigate the serologic type, phage-biotype and toxic factor of Vibrio cholerae isolated from different sea products, analyze the relation between the Vibrio cholerae in sea products and cholera epidemiology, and provide references for forecasting cholera epidemic situation and drawing out a preventing plan.


METHODS
The biotype of strains isolated was analyzed by using type and phage-biotype serological methods. The toxic gene was detected by PCR.


RESULTS
The constituent ratio of V. cholerae O139, Ogawa and Inaba were, respectively, 48.44%, 20.31% and 31.25% in 64 strains of V. cholerae. The result of phage-biotype showed that the 26 strains of V. cholerae O1 were all non-epidemic strains. The result of toxic gene detecting showed that positive rate of V. cholerae O139 was higher than those of Ogawa and Inaba.


CONCLUSIONS
The positive rate of toxic gene in V. cholerae O139 was high and the V. cholerae O139 was mainly in turtle, breed aquatics water and crustacean, so these sea products were the important sectors in cholera prevention and control.
Chang
Zhao-rui
ZR
Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Zhang
Jing
J
Wang
Duo-chun
DC
Zhong
Hao-jie
HJ
Xu
Jin
J
Ran
Lu
L
Wang
Mao-wu
MW
Wang
Zi-jun
ZJ
Kan
Biao
B
D000818:Animals; D001434:Bacteriophage Typing; D004269:DNA, Bacterial / Q000235:genetics; D017747:Seafood / Q000382:microbiology*; D012703:Serotyping; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D041241:Vibrio cholerae O1 / Q000302:isolation & purification; D041242:Vibrio cholerae O139 / Q000302:isolation & purification
D004269:DNA, Bacterial