Molecular typing divides marine mammal strains of Brucella into at least three groups with distinct host preferences.
In order to investigate the genetic relationships within Brucella isolated from marine mammals, two genome-based typing methods, variable number of tandem repeats (VNTR) typing and multilocus sequence analysis (MLSA), were applied to a selection of 74 marine mammal isolates. All isolates were examined by VNTR and data were compared with multilocus sequencing data from a subset of 48 of these. Marine mammal brucellae are distinct from classically recognized species by these methods and appear to correspond to three major genetic groups, which reflect distinct preferred hosts. One group contains isolates predominantly found in pinnipeds (seals) and corresponds to the previously proposed species 'Brucella pinnipediae'. However, isolates corresponding to the previously proposed species 'Brucella cetaceae' fall into two distinct groups that appear to have different preferred cetacean hosts (porpoises and dolphins). Furthermore, these two groups appear less closely related to each other than either group is to 'B. pinnipediae' isolates. The groups identified by VNTR typing and MLSA are completely congruent. The relevance of these findings to current proposals to recognize two species of marine mammal Brucella is discussed.
Groussaud
Pauline
P
Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK.
Shankster
Stephen J
SJ
Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK.
Koylass
Mark S
MS
Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK.
Whatmore
Adrian M
AM
Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK.
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D002002:Brucella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002006:Brucellosis / Q000382:microbiology*; D002591:Cetacea / Q000382:microbiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D005838:Genotype; D018598:Minisatellite Repeats / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial