title: Dentin demineralization when subjected to BioPure MTAD: a longitudinal and quantitative assessment.
abstract: In the present study, the demineralizing ability of BioPure MTAD (Dentsply/Tulsa, Tulsa, OK), 17% EDTA, and 5% citric acid on radicular dentin was quantitatively and longitudinally analyzed. 3 mm thick disks were obtained at the root cervical third from 9 maxillary human molars, and a standardized smear layer was produced. Cosite images of the dentin surface were obtained after several cumulative demineralization times. Sixteen images were obtained in each dentin sample for each experimental time, at 1000x magnification. An image processing and analysis sequence measured sets of images, providing data of area fraction for thousands of tubules over time. Thus, it was possible to follow the demineralization phenomenon and quantitatively analyze the effect of the various substances. The nonparametric Kruskal-Wallis H-test was used to analyze the data. Based on the present results, it can be concluded that the demineralization kinetics promoted by both 5% CA and BioPure MTAD was significantly faster than by 17% EDTA.
authors.affiliation: Veiga de Almeida University, Rio de Janeiro, Brazil.
authors.forename: Gustavo
authors.initials: G
authors.lastname: De-Deus
authors.forename: Claudia
authors.initials: C
authors.lastname: Reis
authors.forename: Sandra
authors.initials: S
authors.lastname: Fidel
authors.forename: Rivail
authors.initials: R
authors.lastname: Fidel
authors.forename: Sidnei
authors.initials: S
authors.lastname: Paciornik
mesh_terms: D002614:Chelating Agents / Q000494:pharmacology*; D019343:Citric Acid / Q000494:pharmacology*; D003648:Decalcification Technique; D003804:Dentin / Q000737:chemistry* / Q000187:drug effects* / Q000648:ultrastructure; D003806:Dentin Permeability; D004318:Doxycycline / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008963:Molar; D011136:Polysorbates / Q000494:pharmacology*; D012388:Root Canal Irrigants / Q000494:pharmacology*; D016369:Smear Layer; D018709:Statistics, Nonparametric; D013997:Time Factors
chemical_list: D002614:Chelating Agents; C475043:MTAD (intracanal irrigant); D011136:Polysorbates; D012388:Root Canal Irrigants; D019343:Citric Acid; D004492:Edetic Acid; D004318:Doxycycline