Cyclic changes in metabolic state during the life of a yeast cell.
Budding yeast undergo robust oscillations in oxygen consumption during continuous growth in a nutrient-limited environment. Using liquid chromatography-mass spectrometry and comprehensive 2D gas chromatography-mass spectrometry-based metabolite profiling methods, we have determined that the intracellular concentrations of many metabolites change periodically as a function of these metabolic cycles. These results reveal the logic of cellular metabolism during different phases of the life of a yeast cell. They may further indicate that oscillation in the abundance of key metabolites might help control the temporal regulation of cellular processes and the establishment of a cycle. Such oscillations in metabolic state might occur during the course of other biological cycles.
Tu
Benjamin P
BP
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9038, USA. benjamin.tu@utsouthwestern.edu
Mohler
Rachel E
RE
Liu
Jessica C
JC
Dombek
Kenneth M
KM
Young
Elton T
ET
Synovec
Robert E
RE
McKnight
Steven L
SL
D000105:Acetyl Coenzyme A / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006418:Heme / Q000096:biosynthesis; D009249:NADP / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism; D013997:Time Factors
D006418:Heme; D009249:NADP; D013455:Sulfur; D000105:Acetyl Coenzyme A