The influence of the cell-adhesive proteins E-cadherin and fibronectin embedded in carbonate-apatite DNA carrier on transgene delivery and expression in a mouse embryonic stem cell line.
Stem cells have the potential to be differentiated to a specific cell type through genetic manipulation and therefore, represent a new and versatile source of cell replacement in regenerative medicine. However, conventional ways of gene transfer to these progenitor cells, suffer from a number of disadvantages particularly involving safety and efficacy issues. We have recently reported on the development of a bio-functionalized DNA carrier of carbonate apatite by embedding fibronectin and E-cadherin chimera on the carrier, leading to its high-affinity interactions with embryonic stem cell surface and accelerated transgene delivery for subsequent expression. Here, we show the molecular basis of synthesizing highly functional composite particles utilizing DNA, cell-adhesive proteins and inorganic crystals, and finally establish a superior transfection system for a mouse stem cell line having potential applications in cell-based therapy.
Kutsuzawa
K
K
Global Edge Institute, Tokyo Institute of Technology, 2-12-1, E3-1 Ookayama, Meguro, Tokyo 152-8550, Japan.
Akaike
T
T
Chowdhury
Ezharul H
EH
D000818:Animals; D001031:Apatites / Q000737:chemistry* / Q000494:pharmacology; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D003460:Crystallization; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D005353:Fibronectins / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D014162:Transfection / Q000379:methods*
D001031:Apatites; D015820:Cadherins; D005353:Fibronectins; C030782:carboapatite; D004247:DNA