title: Phospholipase A(1) assays using a radiolabeled substrate and mass spectrometry.
abstract: Although a number of phospholipase A(1)s (PLA(1)s) have been identified in the recent decade, the physiological functions of PLA(1)s remain almost elusive. The major reason for this is the poor availability of assay methods. In many studies, radiolabeled phospholipid substrates have been used to measure PLA(1) activity. This chapter describes the conventional PLA(1) assay using a radiolabeled substrate and a novel PLA(1) assay using electrospray ionization mass spectrometry.
authors.affiliation: Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Rei
authors.initials: R
authors.lastname: Morikawa
authors.forename: Masafumi
authors.initials: M
authors.lastname: Tsujimoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Arai
authors.forename: Junken
authors.initials: J
authors.lastname: Aoki
mesh_terms: D000818:Animals; D002250:Carbon Radioisotopes; D017815:Elapidae; D006367:HeLa Cells; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D007553:Isotope Labeling / Q000379:methods; D008322:Mammals; D013058:Mass Spectrometry; D054466:Phospholipases A1 / Q000032:analysis*; D054467:Phospholipases A2 / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D002250:Carbon Radioisotopes; D007527:Isoenzymes; D010743:Phospholipids; D011994:Recombinant Proteins; D054466:Phospholipases A1; D054467:Phospholipases A2