Effect of heparin on production of basic fibroblast growth factor and transforming growth factor-beta1 by human normal skin and hyperplastic scar fibroblasts.
Heparin affects both dermal fibroblast proliferation and collagen and may mediate these effects by altering the levels of basic fibroblast growth factor (bFGF) and transforming growth factor-beta1 (TGF-beta1) production as a wound healing modulator. The purpose of this study is to probe the effect of heparin on bFGF and TGF-beta1 production by human normal skin and hyperplastic scar fibroblasts. This research investigates the effect of heparin on bFGF and TGF-beta1 production by human normal skin and hyperplastic scar fibroblasts with exposure to 0, 100, 300, or 600 microg/ml heparin for 24, 48, 72, or 96 hours in a serum-free in vitro model. Levels of bFGF and TGF-beta1 in the supernatants were determined by enzyme-linked immunosorbant assay. All doses of heparin significantly stimulated production of bFGF by normal skin (393% to 1019% increase) and hyperplastic scar fibroblasts (405% to 899% increase) at all time points (P < .05). Heparin (300 and 600 microg/ml) also stimulated TGF-beta1 production by normal skin (26% to 83%) and hyperplastic scar fibroblasts (63% to 85%) with statistical significance (P < .05) at various time points. These effects of heparin on normal skin and hyperplastic scar fibroblasts may have implications for hyperplastic scar formation and wound healing in vivo.
Fan
Shu-Qiang
SQ
Department of Burns and Plastic Surgery, The Second Affiliated Hospital of Shandong University, Shandong, China.
Qin
Li-Yan
LY
Cai
Jing-Long
JL
Zhu
Gui-Ying
GY
Bin
Xu
X
Yan
Hong-Shan
HS
D000328:Adult; D002921:Cicatrix / Q000188:drug therapy* / Q000517:prevention & control; D003094:Collagen / Q000187:drug effects; D005346:Fibroblast Growth Factors / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects*; D006493:Heparin / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D006965:Hyperplasia / Q000517:prevention & control*; D066298:In Vitro Techniques; D012867:Skin / Q000187:drug effects*; D053773:Transforming Growth Factor beta1 / Q000187:drug effects*; D014945:Wound Healing / Q000502:physiology*
D053773:Transforming Growth Factor beta1; D005346:Fibroblast Growth Factors; D006493:Heparin; D003094:Collagen