title: Serum protein oxidation and apolipoprotein CIII levels in people with systemic lupus erythematosus with and without nephritis.
abstract: Increased oxidative stress is a hallmark of the autoimmune disease systemic lupus erythematosus (SLE). This study compares serum protein oxidation levels in SLE patients without and with renal involvement (lupus nephritis); the latter have a significantly poorer prognosis. Similar increases in protein carbonyls and decreases in protein thiols were observed in both SLE groups compared to controls. Protein carbonyl distribution, determined by Western blotting of 2D gels, was similar in both SLE groups, suggesting factors other than oxidation also play a role in SLE complications. 2D electrophoresis examined the serum proteome further. Six proteins were significantly decreases in non-renal SLE patients compared to controls; five were identified by mass spectrometry, including one isoform of pro-atherogenic apoCIII. Total apoCIII levels (assessed by ELISA) in lupus nephritis patients were significantly elevated compared to controls or non-renal SLE patients. Thus, levels of oxidized proteins and apoCIII may be useful biomarkers in SLE studies.
authors.affiliation: Free Radical Group, The Heart Research Institute, Camperdown, Sydeny, Australia. morganp@hri.org.au
authors.forename: Philip E
authors.initials: PE
authors.lastname: Morgan
authors.forename: Allan D
authors.initials: AD
authors.lastname: Sturgess
authors.forename: Annemarie
authors.initials: A
authors.lastname: Hennessy
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Davies
mesh_terms: D000328:Adult; D053305:Apolipoprotein C-III / Q000097:blood* / Q000302:isolation & purification; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000302:isolation & purification / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000145:classification / Q000150:complications / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D009393:Nephritis / Q000097:blood* / Q000209:etiology; D010084:Oxidation-Reduction; D020543:Proteome
chemical_list: D053305:Apolipoprotein C-III; D015415:Biomarkers; D001798:Blood Proteins; D020543:Proteome