Determination of loratadine and pseudoephedrine sulfate in pharmaceuticals based on non-linear second-order spectrophotometric data generated by a pH-gradient flow injection technique and artificial neural networks.
Loratadine (LOR) and pseudoephedrine sulfate (PES) were determined in pharmaceutical samples by using non-linear second-order data generated by a pH-gradient flow injection analysis (FIA) system with diode-array detection. Determination of both analytes was performed on the basis of differences between the acid-base and spectral features of each drug species. Non-linearities were detected by using both qualitative and quantitative tools. As a consequence of the non-linearity, a recently reported algorithm, artificial neural networks followed by residual bilinearization (ANN/RBL), was shown to furnish more satisfactory results. Recoveries of 99.7% (LOR) and 95.6% (PES) were obtained when analyzing a validation set containing unexpected components (the usual excipients found in pharmaceutical preparations). The average value obtained by implementation of the method on four replicates was compared with that obtained by a reference method based on HPLC (difference not significant; p > 0.05).
Culzoni
María J
MJ
Laboratorio de Desarrollo Analítico y Quimiometría, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, Santa Fe, (S3000ZAA), Argentina.
Goicoechea
Héctor C
HC
D000465:Algorithms; D018926:Anti-Allergic Agents / Q000032:analysis; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004809:Ephedrine / Q000032:analysis*; D017022:Flow Injection Analysis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D017336:Loratadine / Q000032:analysis*; D008956:Models, Chemical; D016571:Neural Networks, Computer; D013053:Spectrophotometry
D018926:Anti-Allergic Agents; D017336:Loratadine; D004809:Ephedrine