[Separation and identification of chlorogenic acid and related impurities by high performance liquid chromatography-tandem mass spectrometry].
A method using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established for analysis and identification of chlorogenic acid along with its related impurities. A Gemini C18 HPLC column (4.6 mm x 150 mm,5 microm) was used with acetonitrile-water ( containing 0.1% formic acid) (8:92, v/v) as mobile phase. Eight related impurities of chlorogenic acid were identified and their structures were determined by using online HPLC-MS/MS and photodiode array detector (DAD). The method is quick, simple and can be used directly to identify the structure of unknown trace substances in the sample of chlorogenic acid.
Tian
Chenxu
C
West China Pharmaceutical School of Sichuan University, Chengdu 610041, China.
Xu
Xiaoping
X
Liao
Liyun
L
Zhang
Jie
J
Liu
Jing
J
Zhou
Sha
S
D000097:Acetonitriles / Q000737:chemistry; D002726:Chlorogenic Acid / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D014867:Water; D002726:Chlorogenic Acid; C032159:acetonitrile