title: Recombinant proteins and peptides as tools for studying IgE reactivity with low-molecular-weight glutenin subunits in some wheat allergies.
abstract: Two genes of wheat low-molecular-weight glutenin subunits (LMW-GS), B16 and P73, were cloned and expressed in E. coli. They were homologous to proteins encoded respectively at Glu-B3 and Glu-D3 loci. The N-terminal and C-terminal halves of B16 (NB16 and B16C) and the two chimeras combining the halves of the two genes (B16-P73 and P73- B16) were also expressed. All these constructs were compared for their reactivity with IgE from 24 patients suffering from different forms of wheat allergies. The results confirmed that LMW-GSs bound IgE in all adult allergies tested. Strong differences in reactivity between all the constructs were observed. They were disease-dependent. In wheat-dependent exercise-induced anaphylaxis (WDEIA), the reactivity of the constructs depended partly on common epitopes with omega-5 gliadins but also on differences in molecule conformation. The presence of NB16 in the constructs greatly influenced their IgE reactivity.
authors.affiliation: UMR206 Chimie Biologique, AgroParisTech, INRA, Thiverval-Grignon, France. Jacques.Snegaroff@grignon.inra.fr.
authors.forename: Jacques
authors.initials: J
authors.lastname: Snégaroff
authors.forename: Gérard
authors.initials: G
authors.lastname: Branlard
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bouchez-Mahiout
authors.forename: Béatrice
authors.initials: B
authors.lastname: Laudet
authors.forename: Martina
authors.initials: M
authors.lastname: Tylichova
authors.forename: Thierry
authors.initials: T
authors.lastname: Chardot
authors.forename: Catherine
authors.initials: C
authors.lastname: Pecquet
authors.forename: Dominique
authors.initials: D
authors.lastname: Choudat
authors.forename: N
authors.initials: N
authors.lastname: Raison-Peyron
authors.forename: M
authors.initials: M
authors.lastname: Vigan
authors.forename: S
authors.initials: S
authors.lastname: Kerre
authors.forename: Michel
authors.initials: M
authors.lastname: Laurière
mesh_terms: D000595:Amino Acid Sequence; D000707:Anaphylaxis / Q000276:immunology; D002678:Chimera; D004926:Escherichia coli; D015444:Exercise; D005983:Glutens / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D021182:Wheat Hypersensitivity / Q000175:diagnosis / Q000276:immunology*
chemical_list: D010940:Plant Proteins; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D005983:Glutens; C037302:glutenin