Construction and characterization of soft-supported lipid bilayer membranes for biosensors application.
A model cell membrane can be ingeniously used to mimic biological processes with wide applications in the biomedical field. For instance, a model cell membrane can be used as a functionalized element integrated to a sensor for identification of specific proteins or other molecules in a biofluid. A special characteristic of the model membrane described in this work is the fluidic or surface mobility feature given by a thin film of grafted polymer that serves as a cushion layer to support lipid bilayers. Lipid bilayers were deposited on a polyethylene glycol (PEG) thin film over a polished glass substrate using the Langmuir-Blodgett technique. Topographical characterization of the bilayers was accomplished using atomic force microscopy (AFM). Results revealed that the grafted polymeric PEG cushion layer confers mobility to the model membrane.
Jimenez
Jeffy
J
Dept. of Chem. Eng., Univ. of South Florida, Tampa, FL 33620, USA. jimenezj@eng.usf.edu
Heim
August J
AJ
Matthews
W Garrett
WG
Alcantar
Norma
N
D015374:Biosensing Techniques; D002462:Cell Membrane / Q000737:chemistry* / Q000502:physiology*; D005898:Glass; D008051:Lipid Bilayers / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008954:Models, Biological; D011092:Polyethylene Glycols
D008051:Lipid Bilayers; D011092:Polyethylene Glycols