A serial dilution microfluidic device for cytotoxicity assays.
A novel microfluidic device is presented which creates a linear serial dilution of two input fluid streams. This platform facilitates higher productivity as a component of a high throughput cytotoxicity testing strategy. A modeling solution is presented to create custom linear dilution schemes. The featured device creates a serial dilution of two solutions in the range of 1:9 through 9:1 across nine discrete dilutions. It has been validated to create a highly linear progression of dilutions with an R2 value of 0.9993. The device functions equivalently over a wide range of flow rates. The standard deviation of dilution values averages 0.76% over six flow rates spanning 0.5 to 16 microl min(-1).
O'Neill
Adrian T
AT
Dept. of Biomed. Eng., North Carolina Univ., Chapel Hill, NC, USA. atoneill@ncsu.edu
Monteiro-Riviere
Nancy
N
Walker
Glenn M
GM
D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D018675:Toxicity Tests / Q000295:instrumentation* / Q000379:methods