Berberine inhibits TPA-induced MMP-9 and IL-6 expression in normal human keratinocytes.
Berberine is a plant ingredient that has anti-inflammatory and anti-oxidative effects. Matrix metalloproteinase-9 (MMP-9) and interleukin-6 (IL-6) are known to be highly induced by ultraviolet (UV) light and may play important roles in UV-induced skin inflammation and the skin aging process. In this study, we investigated the effects of berberine on MMP-9 and IL-6 expression in normal human keratinocytes (NHK). Our results demonstrated that berberine dose-dependently inhibited basal and TPA-induced expression and activity of MMP-9, and also suppressed TPA-induced IL-6 expression. Berberine prevented TPA-induced ERK activation and AP-1 DNA binding activity. Therefore, berberine may be used as an effective ingredient for anti-skin aging products, which can prevent skin inflammation and the degradation of extracellular matrix proteins, including collagen, by MMPs.
Kim
Sangmin
S
Department of Dermatology, Seoul National University College of Medicine, Seoul, Republic of Korea.
Kim
Yoonkyung
Y
Kim
Ji Eun
JE
Cho
Kwang Hyun
KH
Chung
Jin Ho
JH
D001599:Berberine / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D015394:Molecular Structure; D011725:Pyridines / Q000633:toxicity*; D000093242:Interleukin-6 Inhibitors
D001599:Berberine; D015850:Interleukin-6; D020780:Matrix Metalloproteinase 9; D061965:Matrix Metalloproteinase Inhibitors; D011725:Pyridines; D000093242:Interleukin-6 Inhibitors; C431843:tris(2-pyridylmethyl)amine