The inhibition of in vivo tumorigenesis of osteosarcoma (OS)-732 cells by antisense human osteopontin RNA.
Osteopontin (OPN) is a secreted, non-collagenous, sialic acid-rich protein which functions by mediating cell-matrix interactions and cellular signaling via binding with integrins and CD44 receptors. An increasing number of studies have shown that OPN plays an important role in controlling cancer progression and metastasis. OPN was found to be expressed in many human cancer types, and in some cases, its over-expression was shown to be directly associated with poor patient prognosis. In vitro cancer cell line and animal model studies have clearly indicated that OPN can function in regulating the cell signaling that ultimately controls the oncogenic potential of various cancers. Previous studies in our laboratory demonstrated that OPN is highly expressed in human osteosarcoma (OS) cell line OS-732. In this study, we successfully reduced the tumorigenecity of OS-732 cells xenotransplanted into nude mice, using the antisense human OPN (hOPN) RNA expression vector.
Liu
Si-Jin
SJ
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China. s jliu@mit.edu
Zhang
Dao-Qiang
DQ
Sui
Xiu-Mei
XM
Zhang
Lin
L
Cai
Zi-Wei
ZW
Sun
Li-Qiu
LQ
Liu
Ya-Jun
YJ
Xue
Yan
Y
Hu
Guo-Fa
GF
D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053495:Osteopontin / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D016372:RNA, Antisense / Q000627:therapeutic use*
D016372:RNA, Antisense; C506895:SPP1 protein, human; D053495:Osteopontin