Quorum sensing detected by atomic force microscopy imaging of corrals surrounding multicellular arrangement of bacteria.
Connectivity of the glycocalyx covering of small communities of Acidithiobacillus ferrooxidans bacteria deposited on hydrophilic mica plates was imaged by atomic force microscopy. When part of the coverage was removed by water rinsing, an insoluble structure formed by corrals surrounding each individual bacterium was observed. A collective ring structure with clustered bacteria (>or=3) was observed, which indicates that the bacteria perceived the neighborhood in order to grow a protective structure that results in smaller production of exopolysaccharides material. The most surprising aspect of these collective corral structures was that they occur at a low bacterial cell density. The deposited layers were also analyzed by confocal Raman microscopy and shown to contain polysaccharides, protein, and glucoronic acid.
Teschke
Omar
O
Laboratório de Nanoestruturas e Interfaces, Instituto de Fisica, UNICAMP, 13083-970, Campinas, SP, Brazil. oteschke@ifi.unicamp.br
De Souza
Elizabeth Fátima
EF
Silva-Stenico
Maria Estela
ME
Fiore
Marli De Fátima
MF
Etchegaray
Augusto
A
D042763:Acidithiobacillus / Q000502:physiology*; D001426:Bacterial Proteins / Q000032:analysis; D005937:Glucaric Acid / Q000032:analysis; D019276:Glycocalyx / Q000737:chemistry* / Q000382:microbiology*; D018625:Microscopy, Atomic Force / Q000379:methods*; D018613:Microscopy, Confocal; D011135:Polysaccharides, Bacterial / Q000032:analysis; D053038:Quorum Sensing / Q000502:physiology*
D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial; D005937:Glucaric Acid