Modified oligonucleotides as tools for allele-specific amplification.
Allele-specific polymerase chain reaction (PCR), a method that reports nucleotide variations through either the presence or the absence of a DNA product obtained through PCR amplification, holds the promise to combine target amplification and analysis in one single step. Recently, it has been reported that the selectivity of allele-specific PCR can be significantly increased through the employment of chemically modified primer probes. Here, we report on significant developments of primer probe design and synthesis along this line.
Strerath
Michael
M
Konstanz University, Germany.
Detmer
Ilka
I
Gaster
Jens
J
Marx
Andreas
A
D000483:Alleles; D017931:DNA Primers / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
D017931:DNA Primers