Identification of differentially expressed genes in microsatellite stable HNPCC and sporadic colon cancer.
BACKGROUND
Some sporadic colon cancers and hereditary nonpolyposis colorectal cancer (HNPCC) have been known by a constitutional defect in mismatch gene repair (MMR) and dysfunction in of this MMR system, which lead to an aberrant phenotype that can cause microsatellite instability. However, the clinicopathologic features of still existing microsatellite stable (MSS) colon cancer remain to be investigated.


METHODS
We compared the gene expression patterns of nine tumor tissues of HNPCC and nine tumor tissues of sporadic colon cancer with their adjacent pathologically normal, MSS tissues by modified differential display-polymerase chain reaction, selected four potential marker genes, and confirmed their reproducibility by reverse transcriptase-polymerase chain reaction.


RESULTS
Reg I, MLCK, and MYH11 in tumors of MSS HNPCC showed significant differences in gene expression pattern compared with the adjacent pathologically normal tissues (P = 0.028, P = 0.002, and P = 0.001, respectively). Similar differences in expression patterns for the same set of genes were seen between the sporadic colon cancer group and their adjacent pathologically normal tissues (P = 0.012, P = 0.003, and P = 0.002, respectively).


CONCLUSIONS
We suggest that the three cancer-associated differentially expressed genes in MSS sporadic colon cancer or MSS HNPCC might be potential tumor markers.
Lee
Won-Suk
WS
Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Seo
Gilju
G
Shin
Hee Jung
HJ
Yun
Seong Hyeon
SH
Yun
Haeran
H
Choi
Naeyun
N
Lee
Jinseon
J
Son
Daesoon
D
Cho
Jisook
J
Kim
Jhingook
J
Cho
Yong Beom
YB
Chun
Ho-Kyung
HK
Lee
Woo Yong
WY
D003110:Colonic Neoplasms / Q000235:genetics*; D003123:Colorectal Neoplasms, Hereditary Nonpolyposis / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D051847:Lithostathine / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D018995:Myosin Heavy Chains / Q000235:genetics; D009219:Myosin-Light-Chain Kinase / Q000235:genetics; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction
D051847:Lithostathine; C509903:MYH11 protein, human; C497617:REG1A protein, human; D009219:Myosin-Light-Chain Kinase; D018995:Myosin Heavy Chains