Debittering effect of Actinomucor elegans peptidases on soybean protein hydrolysates.
Effects of the enzymes in Actinomucor elegans extract and the enzyme Alcalase 2.4L on debittering the soybean protein hydrolysates were investigated. When the protein was treated only with the latter, a strong bitterness formed; but it decreased if the protein was treated with both the enzymes. The more the enzymes were used, weaker was the bitterness tasted. SDS-PAGE profile and ESI-MS spectrum of the hydrolysates evidenced that the Alcalase could convert the protein into peptides rapidly, while the enzymes in the A. elegans extract were able to further degrade some peptides which were difficult or unable to be hydrolyzed by the Alcalase. Further systematic analysis of the peptidases showed that the Alcalase exhibited a significant endopeptidase activity towards NBZ-Phe-pNA substrate (p < 0.01), whereas many exopeptidases in the A. elegans extract had the carboxypeptidase activity towards N-CBZ-Ile-Leu (p < 0.01). It is concluded that those exopeptidases presented in the A. elegans extract can benefit by decreasing the bitterness of the soybean protein hydroysate. They are also capable of being used with the Alcalase in a single-step enzymatic reaction to prepare the bitterless protein hydrolysate, which may be an efficient application for food industry.
Li
Li
L
Research and Development Center of Food Proteins, College of Light Industry and Food Science, South China University of Technology, Guangzhou, 510641, China. lili@scut.edu.cn
Yang
Zuo-Yi
ZY
Yang
Xiao-Qun
XQ
Zhang
Gui-He
GH
Tang
Shu-Ze
SZ
Chen
Feng
F
D000328:Adult; D010450:Endopeptidases / Q000378:metabolism; D020689:Exopeptidases / Q000378:metabolism; D005260:Female; D019649:Food Industry / Q000379:methods; D006801:Humans; D008297:Male; D009090:Mucorales / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000378:metabolism*; D011492:Protein Hydrolysates / Q000737:chemistry* / Q000378:metabolism; D030262:Soybean Proteins / Q000378:metabolism*; D013381:Subtilisins / Q000378:metabolism; D013649:Taste; D055815:Young Adult
D011492:Protein Hydrolysates; D030262:Soybean Proteins; D010450:Endopeptidases; D020689:Exopeptidases; D010447:Peptide Hydrolases; D013381:Subtilisins