title: Controlled release of PEI/DNA complexes from PLGA microspheres as a potent delivery system to enhance immune response to HIV vaccine DNA prime/MVA boost regime.
abstract: A novel approach involving the preparation of biodegradable PLGA microspheres containing entrapping complexes of DNA and polyethylenimine was developed to improve the delivery of DNA into antigen-presenting cells after intramuscular injection. Compared to the traditional biodegradable microspheres which release naked DNA, these microspheres released intact and penetrative PEI/DNA complexes over a period of 2 weeks in vitro. In addition, the DNA was not degraded during encapsulation in the PLGA microspheres, owing to the protection of polyethylenimine. After i.m. immunization, the microspheres induced significantly enhanced serum antibody responses 2-3 orders of magnitude greater than naked DNA. Additionally, in contrast to naked DNA, the microspheres induced potent CTL responses at a low dose.
authors.affiliation: College of Life Science, Jilin University, Changchun, PR China.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Zhou
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Xianghui
authors.initials: X
authors.lastname: Yu
authors.forename: Xiao
authors.initials: X
authors.lastname: Zha
authors.forename: Xizhen
authors.initials: X
authors.lastname: Zhang
authors.forename: Xueyun
authors.initials: X
authors.lastname: Wang
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Jin
authors.forename: Yongge
authors.initials: Y
authors.lastname: Wu
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: Yaming
authors.initials: Y
authors.lastname: Shan
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Junqiu
authors.initials: J
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
authors.forename: Jiacong
authors.initials: J
authors.lastname: Shen
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D003692:Delayed-Action Preparations; D005260:Female; D015483:HIV Antibodies / Q000096:biosynthesis; D015497:HIV-1 / Q000276:immunology; D007273:Injections, Intramuscular; D019344:Lactic Acid / Q000008:administration & dosage*; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D011094:Polyethyleneimine / Q000008:administration & dosage*; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics
chemical_list: D016915:AIDS Vaccines; D003692:Delayed-Action Preparations; D015483:HIV Antibodies; D019444:Vaccines, DNA; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011094:Polyethyleneimine