Determination of linezolid in pharmaceutical dosage forms by liquid chromatography and ultraviolet spectroscopy.
A liquid chromatographic (LC) method and a UV spectrophotometric method were developed and validated for quantitative determination of linezolid in tablets and injection. The isocratic LC analyses were performed on an RP18 column using a mobile phase composed of methanol-water-acetonitrile (40 + 40 + 20, v/v/v) at a flow rate of 1.0 mL/min. The UV spectrophotometric method was performed at 251 nm. The analytical methods were validated according to International Conference on Harmonization guidelines. The calibration graphs were linear (correlation coefficient > 0.999) in the studied concentration ranges of 0.1 to 10 microg/mL for LC and 2 to 16 microg/mL for UV spectrophotometry. The relative standard deviation values for intraday and interday precision studies were <2%, and the accuracy was >98% for both methods. The specificity of the LC method was proved using forced degradation. Statistical analysis showed no significant difference between the results obtained by the 2 methods. The proposed methods are precise and accurate and can be applied directly to the oral and parenteral pharmaceutical preparations of linezolid.
Patel
Satish A
SA
Shree S.K. Patel College of Pharmaceutical Education and Research, Ganpat Vidyanagar, Kherava-382 711, Mehsana, Gujarat, India.
Patel
Paresh U
PU
Patel
Natubhai J
NJ
Patel
Madhabhai M
MM
Bangoriya
Urvisha V
UV
D000081:Acetamides / Q000032:analysis* / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000284:Administration, Oral; D000890:Anti-Infective Agents / Q000032:analysis* / Q000737:chemistry; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D000069349:Linezolid; D000432:Methanol / Q000737:chemistry; D008956:Models, Chemical; D023303:Oxazolidinones / Q000032:analysis* / Q000737:chemistry; D010783:Photometry; D012044:Regression Analysis; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014867:Water / Q000737:chemistry
D000081:Acetamides; D000097:Acetonitriles; D000890:Anti-Infective Agents; D023303:Oxazolidinones; D014867:Water; D000069349:Linezolid; D000432:Methanol; C032159:acetonitrile