title: Automated extended volume imaging of tissue using confocal and optical microscopy.
abstract: Conventional histologic techniques cannot readily be used for 3D reconstruction of large tissue volumes. We have developed an imaging rig which supports both confocal and light microscopy, and utilizes a surface imaging approach to serially image embedded tissue blocks while maintaining alignment and registration of the image series.
authors.affiliation: Bioengineering Institute, The University of Auckland, New Zealand. g.sands@auckland.ac.nz
authors.forename: G B
authors.initials: GB
authors.lastname: Sands
authors.forename: D A
authors.initials: DA
authors.lastname: Gerneke
authors.forename: B H
authors.initials: BH
authors.lastname: Smaill
authors.forename: I J
authors.initials: IJ
authors.lastname: Le Grice
mesh_terms: D019412:Anatomy, Cross-Sectional / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D008867:Microtomy / Q000295:instrumentation* / Q000379:methods; D012371:Robotics / Q000295:instrumentation* / Q000379:methods; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods; D013382:Subtraction Technique / Q000295:instrumentation