Comparison of multiple-locus variable-number tandem-repeat analysis with other PCR-based methods for typing Brucella suis isolates.
Multiple-locus variable-number tandem-repeat analysis (MLVA), multiplex PCR, and PCR-restriction fragment length polymorphism analysis were compared for typing Brucella suis isolates. A perfect concordance was obtained among these molecular assays. However, MLVA was the only method to demonstrate brucellosis outbreaks and to confirm that wildlife is a reservoir for zoonotic brucellosis.
García-Yoldi
David
D
Departamento de Microbiología y Parasitología, Universidad de Navarra, c/ Irunlarrea no 1, 31008, Pamplona, Spain.
Le Fleche
Philippe
P
De Miguel
María J
MJ
Muñoz
Pilar M
PM
Blasco
José M
JM
Cvetnic
Zeljko
Z
Marín
Clara M
CM
Vergnaud
Gilles
G
López-Goñi
Ignacio
I
D000818:Animals; D000835:Animals, Wild / Q000382:microbiology; D015373:Bacterial Typing Techniques / Q000379:methods*; D041021:Brucella suis / Q000145:classification* / Q000235:genetics*; D002006:Brucellosis / Q000453:epidemiology / Q000382:microbiology*; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D020080:Tandem Repeat Sequences / Q000235:genetics
D004269:DNA, Bacterial