title: Characteristics of anion channels in the tonoplast of the liverwort Conocephalum conicum.
abstract: Isolated vacuoles of the liverwort Conocephalum conicum thallus cells were investigated using the patch-clamp technique. At high cytosolic Ca(2+) activities, slowly activating currents were evoked by positive potentials. The currents were conducted by the SV (slow-vacuolar) channel. When isolation of vacuoles was carried out at high Mg(2+) and low Ca(2+) concentration and the same proportion of the cations was kept in the bath, currents were recorded at negative potentials. Once activated, these currents persisted even after replacing Mg(2+) with K(+) in the bath. Sr(2+) and Ba(2+) were also effective activators of the currents. With a Cl(-) gradient, 10 mM in the bath and 100 mM in the lumen, currents were significantly reduced and the current-voltage characteristics shifted towards the reversal potential of Cl(-), indicating Cl(-) selectivity. Currents almost vanished after substituting Cl(-) with gluconate. They were strongly reduced by anion channel inhibitors 4,4'-diisothicyanatostilbene-2,2'-disulfonic acid (DIDS; 1 mM), anthracene-9-carboxylic acid (A9C; 2 mM) and ethacrinic acid (0.5 mM). Single-channel recordings revealed a 32 pS channel activating at negative voltages. It is concluded that the currents at negative potentials are carried by anion channels suitable for conducting anions from the cytosol to the vacuole. The anion channels were weakly calcium dependent, remaining active at physiological calcium concentration. The channels were almost equally permeable to Cl(-), NO3(-) and SO4(2-), and much less permeable to malate(2-). Anion channels did not respond to ATP addition. cAMP (10 microM) had a weak effect on anion channels. Protein kinase A (0.4 U) added to the medium caused no significant effect on anion channels.
authors.affiliation: Department of Biophysics, Institute of Biology, Maria Curie-Sklodowska University, Akademicka 19, Lublin, Poland. trebacz@biotop.umcs.lublin.pl
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Trebacz
authors.forename: Gerald
authors.initials: G
authors.lastname: Schönknecht
authors.forename: Halina
authors.initials: H
authors.lastname: Dziubinska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Hanaka
mesh_terms: D000838:Anions; D035926:Hepatophyta / Q000378:metabolism*; D007473:Ion Channels / Q000378:metabolism*; D015388:Organelles / Q000378:metabolism*
chemical_list: D000838:Anions; D007473:Ion Channels