title: [PPARgamma and C/EBPalpha mRNA expression in the primary culture of rat preadipocytes during differentiation].
abstract: To investigate the cell differentiation and the expression profiles of PPARy and C/EBPalpha mRNA, the rat preadipocytes were cultured after dispersed by enzymolysis of collagenase type I and the cell shape were observed under the microscope. The MTT method was adopted to determine the growth curve. The expression levels of PPARgamma and C/EBPalpha mRNA were also detected by relative quantitative RT-PCR. The growth of preadipocytes demonstrated an S-shaped or sigmoid curve. The PPARgamma mRNA in rat preadipocytes showed rather low transcript at day 3, but its expression level markedly increased by 2.5 fold at day 5 and remained at a higher level till day 9. Similarly, the expression level of C/EBPalpha mRNA in rat preadipocytes could hardly be detected at day 3 and dramatically increased at day 7. There was a 2.3 fold increase of C/EBPalpha mRNA expression level at day 7 compared with that at day 5. In a conclusion, this study suggested that both the initiating and maintaining of differentiation process were regulated by PPARgamma, while C/EBPalpha was likely to participate in the maintaining of differentiation process only.
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642.
authors.forename: Sheng Feng
authors.initials: SF
authors.lastname: Chen
authors.forename: Xiao Tong
authors.initials: XT
authors.lastname: Zhu
authors.forename: Gang
authors.initials: G
authors.lastname: Shu
authors.forename: Shu Jing
authors.initials: SJ
authors.lastname: Gao
authors.forename: Ping
authors.initials: P
authors.lastname: Gao
authors.forename: Qing Yan
authors.initials: QY
authors.lastname: Jiang
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D047495:PPAR gamma / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D047495:PPAR gamma; D012333:RNA, Messenger