Electroacoustic miniaturized DNA-biosensor.
A micrometer-sized electroacoustic DNA-biosensor was developed. The device included a thin semi-crystalline polyethylene terephthalate (PET) dielectric layer with two Ag microband electrodes on one side and a DNA thiol-labeled monolayer adsorbed on a gold surface on the other. A resonance wave was observed at 29 MHz with a network analyzer, upon AC voltage application between the two Ag electrodes, corresponding to electromechanical coupling induced by molecular dipoles of the PET polymer chain in the dielectric layer. It was found that the device size and geometry were well adapted to detect DNA hybridization, by measuring the capacity of the resonance response evolution: hybridization induced polarization of the dielectric material that affected the electromechanical coupling established in the dielectric layer. The 0.2 mm(2) sensor sensitive area allows detection in small volumes and still has higher detection levels for bioanalytical applications, the non-contact configuration adopted avoids electric faradic reactions that may damage biosensor sensitive layers, and finally, PET is a costless raw material, easy to process and well adapted for large scale production. The well-balanced technological and economic advantages of this kind of device make it a good candidate for biochip integration.
Gamby
Jean
J
Laboratoire Interfaces et Systèmes Electrochimiques, UPR 15 CNRS, Casier 133, Université Pierre et Marie Curie, Paris Cedex 05, France. gamby@ccr.jussieu.fr
Lazerges
Mathieu
M
Pernelle
Christine
C
Perrot
Hubert
H
Girault
Hubert H
HH
Tribollet
Bernard
B
D000162:Acoustics; D001483:Base Sequence; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D008904:Miniaturization; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D004247:DNA