title: A method for assessment of blood volume parameters in pregnant sheep using fluorescein-labelled dextran.
abstract: The assessment of blood volume parameters in clinical and research settings has been limited by methods that involve radioactivity, complex assays or are unreliable. We aimed to design a method for measuring blood volume parameters that was non-radioactive, simple, cheap and reliable. We have used a commercially available fluorescein-labelled 250kDa dextran, a large inert molecule, and have measured dilution of this through the intravascular space of pregnant ewes. From this estimation of plasma volume and measured hematocrit, we have calculated blood volume and red cell volume. The blood volume results are 6% lower than those obtained using radiolabelled red cells, but there is no significant difference in red cell volume between methods. The coefficient of variation for repeated measurements of plasma volume measurements is 3.8%. This is a simple, reliable, cheap and non-radioactive method for estimating blood volume parameters in pregnant sheep, and may prove useful in other settings.
authors.affiliation: The Liggins Institute, University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors.forename: C W H
authors.initials: CW
authors.lastname: Rumball
authors.forename: P
authors.initials: P
authors.lastname: Van Zijl
authors.forename: M D
authors.initials: MD
authors.lastname: Rutland
authors.forename: F H
authors.initials: FH
authors.lastname: Bloomfield
authors.forename: J E
authors.initials: JE
authors.lastname: Harding
mesh_terms: D000818:Animals; D001811:Blood Volume Determination / Q000379:methods*; D003911:Dextrans / Q000097:blood* / Q000493:pharmacokinetics; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000097:blood*; D034561:Sheep, Domestic / Q000097:blood*
chemical_list: D003911:Dextrans; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate