[Determination of 36 pesticide residues in mushroom by gel permeation chromatography and gas chromatography-tandem mass spectrometry].
A gel permeation chromatographic method was developed for the extraction of 36 pesticides from mushroom prior to gas chromatography with a triple quadrupole mass spectrometric determination. The extraction was based on homogenization with ethyl acetate using a high speed homogenizer. A gel permeation chromatographic clean-up process with ethyl acetate-cyclohexane (1:1, v/v) as mobile phase was applied to the extracts to separate the low-molecular mass pesticides from the high molecular mass compounds in the mushroom. The 7-15 min eluate fraction was collected for subsequent analysis. The target compounds were quantified in the final extract by gas chromatography using a triple quadrupole mass spectrometer with multiple reaction monitoring (MRM). In the linear range (0.01-1.0 mg/L) of each pesticide, the correlation coefficient was higher than 0. 99. The average recoveries (spiked at the levels of 0.01, 0.05, 0.10 mg/kg) were from 72.6% to 117.1% with the relative standard deviations from 2. 0% to 10. 8% (n=5). The limits of detection (LOD) for these pesticides were from 0. 1 to 0.7 microg/kg and the limits of quantification (LOQ) were from 0.2 to 2 Lg/kg.
Yu
Shengliang
S
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean Chemistry, Ocean University of China, Qingdao 266003, China. smarteryu@163.com
Yang
Guipeng
G
Fu
Meng
M
D000363:Agaricales / Q000737:chemistry*; D002850:Chromatography, Gel / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D057230:Limit of Detection; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
D010573:Pesticide Residues