title: Myoblasts isolated from hypertrophy-responsive callipyge muscles show altered growth rates and increased resistance to serum deprivation-induced apoptosis.
abstract: Back and hind limb muscles of sheep paternally heterozygous for the callipyge single nucleotide polymorphism undergo extensive hypertrophy shortly after birth. We have established cell cultures from foetal semitendinosus and longissimus dorsi muscles of normal and callipyge animals. Cultures were assessed for rates of proliferation, cell death, myogenicity and DLK1 expression. Myoblasts from callipyge semitendinosus, but not longissimus dorsi muscles, proliferated faster than myoblasts isolated from normal semitendinosus muscle, and cells isolated from either callipyge muscle were more resistant to serum deprivation-induced apoptosis than equivalent cells isolated from normal individuals. These observations indicate that there are intrinsic differences in the behaviour of isolated myoblasts, which are associated with their muscle and genotype of origin. As myoblasts are the cells responsible for hypertrophy of muscle fibres, the observed differences in cell growth may play a role in the hypertrophy of certain muscles in callipyge animals.
authors.affiliation: School of Veterinary Science, University of Melbourne, Parkville, Australia.
authors.forename: Lopeti T
authors.initials: LT
authors.lastname: Lavulo
authors.forename: Kitipong
authors.initials: K
authors.lastname: Uaesoontrachoon
authors.forename: Michiko
authors.initials: M
authors.lastname: Mirams
authors.forename: Jason D
authors.initials: JD
authors.lastname: White
authors.forename: Noelle E
authors.initials: NE
authors.lastname: Cockett
authors.forename: Eleanor J
authors.initials: EJ
authors.lastname: Mackie
authors.forename: Charles N
authors.initials: CN
authors.lastname: Pagel
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D005260:Female; D047109:Fetal Development; D005434:Flow Cytometry / Q000662:veterinary; D005865:Gestational Age; D006984:Hypertrophy; D008297:Male; D018482:Muscle, Skeletal / Q000166:cytology* / Q000187:drug effects / Q000196:embryology; D009135:Muscular Diseases / Q000196:embryology / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D032446:Myoblasts / Q000473:pathology* / Q000502:physiology; D012757:Sheep Diseases / Q000196:embryology / Q000235:genetics / Q000473:pathology*; D034561:Sheep, Domestic / Q000196:embryology / Q000235:genetics
chemical_list: D016895:Culture Media, Serum-Free