[Effects of NaCl stress on the exogenous gene expression in transgenic poplar plants].
The expressions of BtCry1Ac insect-resistance genes and rhizogenesis genes and their response to NaCl stress were studied using tissue culture plants of high transgenic insect-resistant 'poplar 741' and transpolygenes 741 (insect-resistant genes and T-DNA of Ri plasmid). The results showed that IAA and GA contents increased quickly, plant root number increased and root length reduced after rhizogenesis and hormone synthesis related gene in Ri T-DNA were inserted into the genome of poplar (Figs.2, 3, 8 and 9). Plant height, root number, chlorophyll content, IAA and GA contents decreased gradually with an increase in NaCl stress intensity (Figs.1, 2, 4-6, 8 and 9). Apiece index change extent of transgenic rol gene plant was smaller than transgenic Bt gene plant and non-transgenic plant. Bt toxin protein content of transgenic rol gene plant increased significantly under NaCl stress (Fig.7). Our results indicate that the expressions of the foreign genes changed with the changes of the environmental conditions.
Han
Hui-Jing
HJ
College of Forestry, Hebei Agricultural University, Baoding 071000, China.
Zhang
Fa-Yong
FY
Yang
Min-Sheng
MS
Liang
Hai-Yong
HY
Zhang
Hua-Xin
HX
D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005875:Gibberellins / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D032107:Populus / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*
D005875:Gibberellins; D007210:Indoleacetic Acids; D012965:Sodium Chloride; C030737:indoleacetic acid