Lesional expression of the endogenous angiogenesis inhibitor endostatin/collagen XVIII following traumatic brain injury (TBI).
We have analysed the expression of the endogenous angiogenesis inhibitor endostatin/collagen XVIII following stab wound injury and observed a highly significant (p<0.0001) lesional accumulation confined to areas of pan-necrotic injury and developing secondary damage. Maximal cell numbers were detected at Day 14, declining until Day 21 after injury. Further, endostatin/collagen XVIII(+) monocytic cells accumulated in Virchow-Robin spaces where they formed cell clusters. Besides being prevailingly localised to ED1(+) activated microglia/macrophages, endostatin/collagen XVIII expression was also detected by subendothelial surrounding vessels in the lesioned area. Late and prolonged accumulation of endostatin/collagen XVIII(+) microglia/macrophages and increased numbers of endostatin/collagen XVIII(+) subendothelial cells/vessels in areas of vascular pruning and regression, point to a role in the termination of the transient angiogenic response, linked to a "late" inflammatory milieu.
Mueller
C A
CA
Institute of Brain Research, Medical School, Eberhard-Karls University Tuebingen, Calwerstr. 3, 72076 Tuebingen, Germany. Christian-Andreas.Mueller@ukb.uni-bonn.de
Schluesener
H J
HJ
Fauser
U
U
Conrad
S
S
Schwab
J M
JM
D020533:Angiogenesis Inhibitors / Q000378:metabolism*; D000818:Animals; D001808:Blood Vessels / Q000503:physiopathology; D001921:Brain / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D043170:Collagen Type XVIII / Q000378:metabolism*; D043169:Endostatins / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008297:Male; D017628:Microglia / Q000378:metabolism / Q000473:pathology; D009336:Necrosis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution; D014951:Wounds, Stab / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology
D020533:Angiogenesis Inhibitors; D043170:Collagen Type XVIII; D043169:Endostatins