Determination of eperisone in human plasma by liquid chromatography-ESI-tandem mass spectrometry.
A sensitive and selective method for the determination of 4'-ethyl-3-methyl-3-piperidinopropiophenone hydrochloride (eperisone hydrochloride) in human plasma was developed and validated. The procedure employed an internal standard and a solvent extraction step followed by chromatography on a Xterra C18 minibore column. Detection was by electrospray ionization tandem mass spectrometry with multiple reaction monitoring. The mass transitions of eperisone and tolperisone (IS) were m/z 260 --> 98 and m/z 246 --> 98, respectively. The method has a limit of detection of 0.1 pg/mL for eperisone based on the three times signal-to-noise value with a linear range from 0.01 to 10.0 ng/mL for the analyte. Extraction recovery was on average 98.6 +/- 7.2% (SD) for eperisone. The Intra- and inter-day assay accuracy ranged from 93 to 114% and precision (RSD) was better than 8.5%. The method was successfully employed to analyze plasma samples and evaluate pharmacokinetics of eperisone in healthy male volunteers.
Jeoung
Min Kyo
MK
College of Pharmacy, CBITRC, Chungbuk National University, Cheongju 361-763, Korea.
Jeong
Eun Sook
ES
Kim
Nam Hee
NH
Kim
Chang-Soo
CS
Chung
Youn-Bok
YB
Lee
Yong-Moon
YM
Ahn
Su-Youn
SY
Cho
Hwang-Eui
HE
Lee
Yong Hwa
YH
Hong
Jin Tae
JT
Moon
Dong-Cheul
DC
D000328:Adult; D019540:Area Under Curve; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D011427:Propiophenones / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D011427:Propiophenones; C030848:eperisone