Rapid identification and specific quantification of Staphylococcus epidermidis by 5' nuclease real-time polymerase chain reaction with a minor groove binder probe.
A species-specific quantitative detection method involving 5' nuclease real-time polymerase chain reaction using a minor groove binder probe that was designed from the sodA gene was developed for Staphylococcus epidermidis. This method distinguished S. epidermidis from other staphylococci and specifically quantified the bacterium. This study shows that the method is useful for the identification and quantitative detection of S. epidermidis.
Iwase
Tadayuki
T
Department of Bacteriology, The Jikei University School of Medicine, Tokyo 105-8461, Japan. iwase.tadayuki@jikei.ac.jp
Hoshina
Sadayori
S
Seki
Keiko
K
Shinji
Hitomi
H
Masuda
Shogo
S
Mizunoe
Yoshimitsu
Y
D001426:Bacterial Proteins / Q000235:genetics; D015169:Colony Count, Microbial / Q000379:methods*; D006801:Humans; D015345:Oligonucleotide Probes / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013212:Staphylococcus epidermidis / Q000235:genetics / Q000302:isolation & purification*; D013482:Superoxide Dismutase / Q000235:genetics
D001426:Bacterial Proteins; D015345:Oligonucleotide Probes; C095500:SodA protein, Bacteria; D013482:Superoxide Dismutase