title: The role of tumor necrosis factor receptor type 1 in orthodontic tooth movement.
abstract: Orthodontic tooth movement is dependent on osteoclast activity. Tumor necrosis factor (TNF)-alpha plays an important role, directly or via chemokine release, in osteoclast recruitment and activation. This study aimed to investigate whether the TNF receptor type 1 (p55) influences these events and, consequently, orthodontic tooth movement. An orthodontic appliance was placed in wild-type mice (WT) and p55-deficient mice (p55(-/-)). Levels of TNF-alpha and 2 chemokines (MCP-1/CCL2, RANTES/CCL5) were evaluated in periodontal tissues. A significant increase in CCL2 and TNF-alpha was observed in both groups after 12 hrs of mechanical loading. However, CCL5 levels remained unchanged in p55(-/-) mice at this time-point. The number of TRAP-positive osteoclasts in p55(-/-) mice was significantly lower than that in WT mice. Also, there was a significantly smaller rate of tooth movement in p55(-/-) mice. Analysis of our data suggests that the TNFR-1 plays a significant role in orthodontic tooth movement that might be associated with changes in CCL5 levels.
authors.affiliation: Department of Orthodontics, Pontifícia Universidade Católica de Minas Gerais, Faculty of Dentistry, Belo Horizonte/MG, Brazil.
authors.forename: I
authors.initials: I
authors.lastname: Andrade
authors.forename: T A
authors.initials: TA
authors.lastname: Silva
authors.forename: G A B
authors.initials: GA
authors.lastname: Silva
authors.forename: A L
authors.initials: AL
authors.lastname: Teixeira
authors.forename: M M
authors.initials: MM
authors.lastname: Teixeira
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D016723:Bone Remodeling / Q000502:physiology*; D018932:Chemokine CCL2 / Q000378:metabolism; D018946:Chemokine CCL5 / Q000378:metabolism; D003799:Dental Stress Analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D010010:Osteoclasts / Q000502:physiology; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000172:deficiency / Q000502:physiology*; D014087:Tooth Movement Techniques; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D018946:Chemokine CCL5; D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha