title: Evaluation of the insecticidal efficacy of wild-type and recombinant baculoviruses.
abstract: A considerable amount of work has been done during the last 20 yr to genetically enhance the efficacy of baculovirus insecticides. Following construction of a genetically altered baculovirus, laboratory bioassays are used to quantify various parameters of insecticidal activity such as the median lethal concentration (or dose) required to kill 50% of infected larvae (LC50 or LD50), median survival time of larvae infected at a fixed dose (ST50), and feeding damage incurred by infected larvae. In this chapter, protocols are described for a variety of bioassays and corresponding data analyses for assessment of the insecticidal activity or host range of baculovirus insecticides. Methods are also provided for baculovirus inoculation of larvae using a microapplicator for determining ST50 or for examining physiological effects.
authors.affiliation: Department of Entomology, Iowa State University, Ames, IA, USA.
authors.forename: Huarong
authors.initials: H
authors.lastname: Li
authors.forename: Bryony C
authors.initials: BC
authors.lastname: Bonning
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001681:Biological Assay / Q000379:methods; D005838:Genotype; D007313:Insecta / Q000166:cytology / Q000235:genetics / Q000821:virology; D007306:Insecticides / Q000378:metabolism*; D007814:Larva / Q000166:cytology / Q000235:genetics / Q000821:virology; D010572:Pest Control, Biological / Q000379:methods*; D011995:Recombination, Genetic
chemical_list: D007306:Insecticides