Comparative and physical mapping of 111 previously reported and 105 new porcine microsatellites.
Here we report radiation hybrid mapping of 105 new porcine microsatellite markers on the IMpRH(7000) radiation hybrid panel. In addition, we searched flanking sequences of these markers, as well as 673 previously reported RH-mapped microsatellite markers, for orthology to human sequences. Eighty-seven new and 111 previously mapped sequences exhibited orthology to human sequences. Using a stringent sequence alignment, 25 microsatellite-flanking sequences were found to be highly similar to genic sequences, whereas 173 were similar to non-genic sequences in the human genome. Five markers were located near known breakpoints of synteny between human and swine.
Alexander
L J
LJ
USDA-ARS, LARRL, Ft Keogh, Miles City, MT 59301, USA. lee.alexander@ars.usda.gov
Juneja
B
B
Shiroma
D
D
Nonneman
D
D
Snelling
W
W
Fahrenkrug
S C
SC
D000818:Animals; D018076:DNA, Complementary / Q000737:chemistry; D005819:Genetic Markers; D006801:Humans; D018895:Microsatellite Repeats; D021101:Radiation Hybrid Mapping; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics*
D018076:DNA, Complementary; D005819:Genetic Markers