title: [Construction and immunogenicity study of DNA vaccine expressing human H5N1 influenza virus hemagglutinin].
abstract: Based on the first isolated human H5N1 influenza virus strain A/Anhui/1/2005 in China, HA and HA1 genes were amplified and cloned into the eukaryotic expression vector pStar. The recombinant plasmids pStar-HA and pStar-HA1 were transfected into COS7 cells. Western blot and IFA showed that the two recombinant DNA plasmids were successfully expressed in eukaryotic cells. BALB/c mice were immunized with the plasmids DNA by intramuscular injection. Anti-HA specific antibody in peripheral blood of immunized mice was tested by ELISA. The results showed that the recombinant plasmids successfully induced the anti-HA humoral immune response, and there was no significant difference between HA and HA1 as immunogen. This work provides basis for future development of novel avian flu vaccine.
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China. lifanghuan99@hotmail.com
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Huan
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Yao
authors.forename: Ai-Jun
authors.initials: AJ
authors.lastname: Chen
authors.forename: Cong-Sheng
authors.initials: CS
authors.lastname: Cheng
authors.forename: Run-Qing
authors.initials: RQ
authors.lastname: Jia
authors.forename: Qing
authors.initials: Q
authors.lastname: Tian
authors.forename: Hong
authors.initials: H
authors.lastname: Bo
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Guo
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Yue-Long
authors.initials: YL
authors.lastname: Shu
authors.forename: Zhi-Qing
authors.initials: ZQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D019556:COS Cells; D002522:Chlorocebus aethiops; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007074:Immunoglobulin G; D007252:Influenza Vaccines; D019444:Vaccines, DNA