title: [Evaluation of new fourth-generation human immunodeficiency virus antigen and antibody detection assay with enzyme-linked fluorescent immunoassay].
abstract: We evaluated the fourth-generation HIV screening assay VIDAS HIV DUOII (DUOII) based on ELFA for simultaneous detection of anti-HIV-1 and anti-HIV-2 antibodies and HIV-1 p24 antigen through comparison with other HIV antigen-antibody detection assays. Materials were 1228 HIV-negative specimens, 95 HIV-antibody-positive specimens, and HIV commercial panels. The specificity of DUOII was 99.8% and sensitivity 100%, detecting all of HIV-1 group M subtype A, B, B', C, D, A/E, F, G, B/D, HIV-1 group O, and HIV-2. The sensitivity test to HIV-1 p24 antigen was 5pg/ mL, higher than other assays. DUOII was equivalent to or superior in detecting results earlier than other assays in an evaluation using 10 commercial HIV-1 seroconversion panels of primary infection. DUOII detects anti-HIV IgM antibody, so no negative sample was found in the second window between p24 antigen disappearance and raised anti-HIV IgG antibody. DUOII has sufficient specificity and sensitivity for HIV screening, and detects primary infection sooner than other assays. These results indicate that DUOII is useful and reliable in HIV screening.
authors.affiliation: Department of Biology, Kanagawa Prefectural Institute of Public Health.
authors.forename: Takako
authors.initials: T
authors.lastname: Shima
authors.forename: Koji
authors.initials: K
authors.lastname: Sudo
authors.forename: Makiko
authors.initials: M
authors.lastname: Kondo
authors.forename: Hanako
authors.initials: H
authors.lastname: Kurai
authors.forename: Hiroko
authors.initials: H
authors.lastname: Sagara
authors.forename: Mitsunobu
authors.initials: M
authors.lastname: Imai
mesh_terms: D005455:Fluorescent Antibody Technique / Q000379:methods*; D015488:HIV Antigens / Q000032:analysis*; D015497:HIV-1 / Q000276:immunology*; D015481:HTLV-I Antibodies / Q000032:analysis*; D015482:HTLV-II Antibodies / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D015488:HIV Antigens; D015481:HTLV-I Antibodies; D015482:HTLV-II Antibodies