title: Regulation of adipocyte differentiation of bone marrow stromal cells by transcription factor GATA-2.
abstract: GATA-2 is a transcription factor expressed in stem and progenitor cells of various cell lineages. In this study, we examined the role of GATA-2 in the differentiation of adipocytes, which are the main components of the microenvironment for hematopoiesis. Suppression of GATA-2 in TBR343, a preadipocytic stromal cell line, resulted in the acceleration of adipocyte differentiation. Conversely, overexpression of GATA-2 induced the resistance of adipocyte differentiation. In addition, regulatory elements of the GATA-2 gene in TBR343 are the same as those in hematopoietic cells. These results suggest that GATA-2 maintains a hematopoietic microenvironment by regulating the differentiation of adipocytes.
authors.affiliation: Department of Hematology and Rheumatology, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Okitsu
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Takahashi
authors.forename: Naoko
authors.initials: N
authors.lastname: Minegishi
authors.forename: Junichi
authors.initials: J
authors.lastname: Kameoka
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Kaku
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sasaki
authors.forename: Hideo
authors.initials: H
authors.lastname: Harigae
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D050989:GATA2 Transcription Factor / Q000502:physiology*; D006410:Hematopoiesis; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D050989:GATA2 Transcription Factor; C494712:Gata2 protein, mouse