Selenium methylselenocysteine protects human hepatoma HepG2 cells against oxidative stress induced by tert-butyl hydroperoxide.
Selenium methylselenocysteine (Se-MeSeCys) is a common selenocompound in the diet with a tested chemopreventive effect. This study investigated the potential protective effect of Se-MeSeCys against a chemical oxidative stress induced by tert-butyl hydroperoxide (t-BOOH) on human hepatoma HepG2 cells. Speciation of selenium derivatives by liquid chromatography-inductively coupled plasma mass spectrometry depicts Se-MeSeCys as the only selenocompound in the cell culture. Cell viability (lactate dehydrogenase) and markers of oxidative status--concentration of reduced glutathione (GSH) and malondialdehyde (MDA), generation of reactive oxygen species (ROS) and activity of the antioxidant enzymes glutathione peroxidase (GPx) and glutathione reductase (GR)--were evaluated. Pretreatment of cells with Se-MeSeCys for 20 h completely prevented the enhanced cell damage, MDA concentration and GR and GPx activity and the decreased GSH induced by t-BOOH but did not prevent increased ROS generation. The results show that treatment of HepG2 cells with concentrations of Se-MeSeCys in the nanomolar to micromolar range confers a significant protection against an oxidative insult.
Cuello
Susana
S
Departamento de Química Analítica, Facultad de Químicas, Universidad Complutense, 28040, Madrid, Spain.
Ramos
Sonia
S
Mateos
Raquel
R
Martín
M Angeles
MA
Madrid
Yolanda
Y
Cámara
Carmen
C
Bravo
Laura
L
Goya
Luis
L
D016588:Anticarcinogenic Agents / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival; D003545:Cysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D005978:Glutathione / Q000032:analysis; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism; D008315:Malondialdehyde / Q000032:analysis; D016566:Organoselenium Compounds / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D017279:Selenocysteine / Q000031:analogs & derivatives; D013997:Time Factors; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
D016588:Anticarcinogenic Agents; D016566:Organoselenium Compounds; D017382:Reactive Oxygen Species; D017279:Selenocysteine; D008315:Malondialdehyde; D020122:tert-Butylhydroperoxide; D005978:Glutathione; D003545:Cysteine; C002979:selenomethylselenocysteine