title: Estimating the distribution of the G(2) phase duration from flow cytometric histograms.
abstract: A mathematical model, based on branching processes, is proposed to interpret BrdUrd DNA FCM-derived data. Our main interest is in determining the distribution of the G(2) phase duration. Two different model classes involving different assumptions on the distribution of the G(2) phase duration are considered. Different assumptions of the G(2) phase duration result in very similar distributions of the S phase duration and the estimated means and standard deviations of the G(2) phase duration are all in the same range.
authors.affiliation: Centre for Mathematical Sciences, Division of Mathematical Statistics, Lund University, 221 00, Lund, Sweden. sara@maths.lth.se
authors.forename: Sara
authors.initials: S
authors.lastname: Larsson
authors.forename: Tobias
authors.initials: T
authors.lastname: Ryden
authors.forename: Ulla
authors.initials: U
authors.lastname: Holst
authors.forename: Stina
authors.initials: S
authors.lastname: Oredsson
authors.forename: Maria
authors.initials: M
authors.lastname: Johansson
mesh_terms: D000465:Algorithms; D001973:Bromodeoxyuridine / Q000032:analysis / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology; D045744:Cell Line, Tumor; D003198:Computer Simulation; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D005434:Flow Cytometry / Q000379:methods*; D016195:G2 Phase / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D016013:Likelihood Functions; D008954:Models, Biological; D016196:S Phase / Q000502:physiology; D016008:Statistical Distributions; D013269:Stochastic Processes
chemical_list: D004247:DNA; D001973:Bromodeoxyuridine