title: [Effects of peroxisome proliferators activated receptors on caveolin-1 expression in foam cells].
abstract: OBJECTIVE
  To study the effect of peroxisome proliferators activated receptors (PPAR) alpha, gamma ligand on ATP-binding cassette transporter A1 (ABCA1) and caveolin-1 expressions and cholesterol, ox-LDL contents in human monocyte derived foam cells.
  
  
  METHODS
  Malondialdehyde (MDA) was measured by TBARS method, ox-LDL detected by ELISA method, cholesterol measured by fluorescence spectrophotometric method, ABCA1, caveolin-1 mRNA and protein expressions determined by RT-PCR and Western blot, in human monocytes, foam cells [human monocyte-derived macrophage induced by myristate acetate (PMA) further treated with 50 mg/L ox-LDL for 24 h], foam cells plus 10 micromol/L pioglitazone for 48 h, foam cells plus 5 micromol/L clofibrate for 48 h.
  
  
  RESULTS
  The intracellular total cholesterol (TC), free cholesterol (FC), cholesteryl ester (CE), ox-LDL and lipid peroxide were significantly increased and the membrane expressions of ABCA1, caveolin-1 were down-regulated in foam cells compared to monocytes (all P < 0.05) and these changes were significantly attenuated by cotreatment with PPARalpha, gamma ligand.
  
  
  CONCLUSIONS
  The anti-atherosclerosis effects of PPARalpha, gamma ligand are related to reducing cholesterol contents and up-regulating ABCA1, caveolin-1 expressions in foam cells.
authors.affiliation: Department of Pathophysiology, Ningxia Medical College, Yinchuan 750004, China. jwcjyd@163.com
authors.forename: Yi-deng
authors.initials: YD
authors.lastname: Jiang
authors.forename: Jian-tuan
authors.initials: JT
authors.lastname: Xiong
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.forename: Gui-zhong
authors.initials: GZ
authors.lastname: Li
authors.forename: Shu-ren
authors.initials: SR
authors.lastname: Wang
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D051242:Caveolin 1 / Q000378:metabolism*; D002460:Cell Line; D002784:Cholesterol / Q000235:genetics / Q000378:metabolism*; D005487:Foam Cells / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism; D047493:PPAR alpha / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism*
chemical_list: C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D051242:Caveolin 1; D047493:PPAR alpha; D047495:PPAR gamma; D008315:Malondialdehyde; D002784:Cholesterol