title: Selective incorporation of polyanionic molecules into hamster prions.
abstract: The central pathogenic event of prion disease is the conformational conversion of a host protein, PrPC, into a pathogenic isoform, PrPSc. We previously showed that the protein misfolding cyclic amplification (PMCA) technique can be used to form infectious prion molecules de novo from purified native PrPC molecules in an autocatalytic process requiring accessory polyanions (Deleault, N. R., Harris, B. T., Rees, J. R., and Supattapone, S. (2007) Proc. Natl. Acad. Sci. U. S. A. 104, 9741-9746). Here we investigated the molecular mechanism by which polyanionic molecules facilitate infectious prion formation in vitro. Ina PMCA reaction lacking PrPSc template seed, synthetic polyA RNA molecules induce hamster HaPrPC to adopt a protease-sensitive, detergent-insoluble conformation reactive against antibodies specific for PrPSc. During PMCA, labeled nucleic acids form nuclease-resistant complexes with HaPrP molecules. Strikingly, purified HaPrPC molecules subjected to PMCA selectively incorporate an approximately 1-2.5-kb subset of [32P]polyA RNA molecules from a heterogeneous mixture ranging in size from approximately 0.1 to >6 kb. Neuropathological analysis of scrapie-infected hamsters using the fluorescent dye acridine orange revealed that RNA molecules co-localize with large extracellular HaPrP aggregates. These findings suggest that polyanionic molecules such as RNA may become selectively incorporated into stable complexes with PrP molecules during the formation of native hamster prions.
authors.affiliation: Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
authors.forename: James C
authors.initials: JC
authors.lastname: Geoghegan
authors.forename: Pablo A
authors.initials: PA
authors.lastname: Valdes
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Orem
authors.forename: Nathan R
authors.initials: NR
authors.lastname: Deleault
authors.forename: R Anthony
authors.initials: RA
authors.lastname: Williamson
authors.forename: Brent T
authors.initials: BT
authors.lastname: Harris
authors.forename: Surachai
authors.initials: S
authors.lastname: Supattapone
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D002384:Catalysis; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D008297:Male; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D018621:PrPC Proteins / Q000737:chemistry / Q000378:metabolism*; D018620:PrPSc Proteins / Q000737:chemistry / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D012260:Ribonucleases / Q000737:chemistry; D012608:Scrapie / Q000378:metabolism* / Q000473:pathology
chemical_list: D000682:Amyloid; D000071228:Polyelectrolytes; D011108:Polymers; D018621:PrPC Proteins; D018620:PrPSc Proteins; D012313:RNA; D012260:Ribonucleases