title: Full-length genome sequences of hepatitis C virus subtype 4f.
abstract: Hepatitis C virus genotype 4 (HCV-4) is very common in central Africa, prevalent in the Middle East, and is becoming increasingly frequent among southern Europeans. We have determined the complete nucleotide sequences of HCV-4f strains and investigated their phylogenetic relationships with other genotypes. We amplified the complete genome of two HCV subtype 4f strains, IFBT84 and IFBT88. The HCV-4f strains have a total of 9181 and 9304 nt, respectively, including the 5' untranslated region followed by a single open reading frame. There was no evidence of genomic recombination in the IFBT84 and IFBT88 strains. The sequences of the HCV-4f strain genomes are closer to those of HCV-1b than to genotypes 2, 3, 5 and 6. It is important to know the full-length sequences of HCV-4 subtypes in order to classify them correctly and to obtain more detailed knowledge about HCV epidemiology and sensitivity to interferon.
authors.affiliation: Facultés de Médecine et de Pharmacie, Université Toulouse III Paul-Sabatier, F-31400 Toulouse, France.|Service de Virologie, CHU de Toulouse, Institut Fédératif de Biologie, F-31059 Toulouse, France.
authors.forename: Fatma
authors.initials: F
authors.lastname: Hmaied
authors.affiliation: Facultés de Médecine et de Pharmacie, Université Toulouse III Paul-Sabatier, F-31400 Toulouse, France.|INSERM, U563, Centre de Physiopathologie de Toulouse Purpan, F-31300 Toulouse, France.|Service de Virologie, CHU de Toulouse, Institut Fédératif de Biologie, F-31059 Toulouse, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Legrand-Abravanel
authors.affiliation: Facultés de Médecine et de Pharmacie, Université Toulouse III Paul-Sabatier, F-31400 Toulouse, France.|INSERM, U563, Centre de Physiopathologie de Toulouse Purpan, F-31300 Toulouse, France.|Service de Virologie, CHU de Toulouse, Institut Fédératif de Biologie, F-31059 Toulouse, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Nicot
authors.affiliation: Facultés de Médecine et de Pharmacie, Université Toulouse III Paul-Sabatier, F-31400 Toulouse, France.
authors.forename: Natalie
authors.initials: N
authors.lastname: Garrigues
authors.affiliation: Facultés de Médecine et de Pharmacie, Université Toulouse III Paul-Sabatier, F-31400 Toulouse, France.|INSERM, U563, Centre de Physiopathologie de Toulouse Purpan, F-31300 Toulouse, France.|Service de Virologie, CHU de Toulouse, Institut Fédératif de Biologie, F-31059 Toulouse, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Chapuy-Regaud
authors.affiliation: INSERM, U563, Centre de Physiopathologie de Toulouse Purpan, F-31300 Toulouse, France.|Service de Virologie, CHU de Toulouse, Institut Fédératif de Biologie, F-31059 Toulouse, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Dubois
authors.affiliation: Laboratoire de Virologie, Centre Pasteur du Cameroun, Cameroon.|Facultés de Médecine et de Pharmacie, Université Toulouse III Paul-Sabatier, F-31400 Toulouse, France.|Service de Virologie, CHU de Toulouse, Institut Fédératif de Biologie, F-31059 Toulouse, France.
authors.forename: Richard
authors.initials: R
authors.lastname: Njouom
authors.affiliation: Facultés de Médecine et de Pharmacie, Université Toulouse III Paul-Sabatier, F-31400 Toulouse, France.|INSERM, U563, Centre de Physiopathologie de Toulouse Purpan, F-31300 Toulouse, France.|Service de Virologie, CHU de Toulouse, Institut Fédératif de Biologie, F-31059 Toulouse, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Izopet
authors.affiliation: Facultés de Médecine et de Pharmacie, Université Toulouse III Paul-Sabatier, F-31400 Toulouse, France.|INSERM, U563, Centre de Physiopathologie de Toulouse Purpan, F-31300 Toulouse, France.|Service de Virologie, CHU de Toulouse, Institut Fédératif de Biologie, F-31059 Toulouse, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Pasquier
mesh_terms: D020121:5' Untranslated Regions; D001483:Base Sequence; D016679:Genome, Viral; D016174:Hepacivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D020121:5' Untranslated Regions