title: Altered expression of CDX-2, PDX-1 and mucin core proteins in "Ulcer-associated cell lineage (UACL)" in Crohn's disease.
abstract: The ulcer-associated cell lineage (UACL) induced at the site of ileac chronic ulceration in Crohn's disease has been reported to show histological differentiation resembling gastric pyloric mucosa. To clarify the significance of homeobox gene-encoded transcription factors in the formation of the UACL in Crohn's disease, we investigated the immunohistochemical expression of gastrointestinal mucins (MUC5AC for gastric surface mucous cells; MUC6 for gastric gland mucous cells, and MUC2 for intestinal goblet cells) and homeobox gene-encoded transcription factors (CDX-2 for intestinal mucosa and PDX-1 for pyloric mucosa) in the UACL. The analysis was undertaken on ileal mucosa obtained from ileal resections performed in 19 patients with active Crohn's disease of the small bowel. The UACL was observed in nine patients. In the UACL, expression of mucous cells with a foveolar-structure showed immunoreactivity to MUC5AC, and the mucous cells with a glandular structure showed immunoreactivity to MUC6, and the expression of MUC2 was decreased. In addition, we detected the decreased expression of CDX-2 along with the increased expression of PDX-1 in the UACL. The UACL showed histological differentiation simulating gastric pylori mucosa. The down-regulation of CDX-2 and the up-regulation of PDX-1 could be an important mechanism in the induction of the UACL.
authors.affiliation: Department of Medicine, Gastroenterology, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Kaneko
authors.forename: Takamichi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Hayama
authors.forename: Noriko
authors.initials: N
authors.lastname: Hosaka
authors.forename: Taiji
authors.initials: T
authors.lastname: Akamatsu
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Ota
mesh_terms: D000071616:CDX2 Transcription Factor; D003424:Crohn Disease / Q000378:metabolism* / Q000473:pathology*; D005753:Gastric Mucosa / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D006651:Histocytochemistry; D018398:Homeodomain Proteins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007082:Ileum / Q000737:chemistry / Q000378:metabolism; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D008679:Metaplasia; D055271:Mucin 5AC; D055262:Mucin-2; D055272:Mucin-6; D009077:Mucins / Q000032:analysis* / Q000378:metabolism; D015534:Trans-Activators / Q000032:analysis / Q000378:metabolism*
chemical_list: D000071616:CDX2 Transcription Factor; D018398:Homeodomain Proteins; C524625:MUC2 protein, human; C524678:MUC5AC protein, human; C524734:MUC6 protein, human; D055271:Mucin 5AC; D055262:Mucin-2; D055272:Mucin-6; D009077:Mucins; D015534:Trans-Activators; C084010:pancreatic and duodenal homeobox 1 protein