title: [Prokaryotic expression of HLA-B * 2705 heavy chain and identification for its activity].
abstract: In order to investigate the expression of heavy chain of HLA-B * 2705 in prokaryotic system and identify its activity, the extra-membrane gene fragment of HLA-B * 2705 was amplified from full-length HLA-B*2705 cDNA by PCR and cloned into pGEM-T vector. After identification by sequencing, the prokaryotic expressing vector pET32a (+)-B * 2705 was constructed. The antigenic activity of expressed protein was identified by Western blot and antibody blocking reaction. The results indicated that the fused HLA-B * 2705 protein expression with high efficiency was obtained. The expressed product was more than 50% of the total bacteria protein. The antigenic activity of expressed protein was confirmed by Western blot and antibody blocking reaction. It is concluded that HLA-B * 2705 fusion protein are obtained as basis for the further studies.
authors.affiliation: Department of Immunology, Affiliated Hospital of Academy of Military Medical Sciences, Laboratory of Immunoassy, National Center of Biomedical Analysis, Beijing 100039, China.
authors.forename: Yan-Bo
authors.initials: YB
authors.lastname: Li
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Sun
authors.forename: Si-Qi
authors.initials: SQ
authors.lastname: Guo
authors.forename: Ye-Ping
authors.initials: YP
authors.lastname: Sun
authors.forename: Xiu-Yun
authors.initials: XY
authors.lastname: Zhang
authors.forename: Fan-Hua
authors.initials: FH
authors.lastname: Kong
authors.forename: Yong-Zhi
authors.initials: YZ
authors.lastname: Xi
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics*; D015796:HLA-B27 Antigen / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: C464936:HLA-B*27:05 antigen; D015796:HLA-B27 Antigen; D007143:Immunoglobulin Heavy Chains