title: Development of oocytes from synchronized follicles of early prepubertal goats under exotic hormone control.
abstract: The aim of this study was to establish whether early prepubertal Sannen dairy goat could provide a large source of ova for SCNT. The effects of different hormonal treatments including untreated control, FSH alone, estadiol plus progesterone (E2-P4), and E2-P4 and FSH (E2-P4-FSH) on ovary size, follicle number and size were studied using the early prepubertal goats aged 39-60 days. Then prepubertal goats aged 39-120 days were categorized into three groups to study the effect of age on recruited follicle number. The meiotic competence of oocytes derived from > or =3 mm follicles recovered from the early prepubertal goats treated with E2-P4-FSH was compared with those treated with FSH alone. Finally, the development competence of the ova from the early goats treated with E2-P4-FSH was evaluated by SCNT. The E2-P4-FSH treatment produced the largest ovaries, the highest numbers of total follicles and follicles > or =3 mm diameter compared with the other treatments. The number of > or =3 mm follicles per goat treated with E2-P4-FSH was significantly higher for those in the age groups 39-60 days than those in the age groups 61-90 days and 91-120 days. The FSH alone treatment resulted in a lower proportion matured ova in vitro within 27 hr than from those goats treated with E2-P4-FSH. Ova derived from the early prepubetal goats resulted in lower rate of blastocyst in SCNT (15.3% versus 22.1%, P < 0.01) than that of adult goat However, the number of ova recovered per goat was substantially greater for the prepubertal goats (108 +/- 10.3 Versus 28 +/- 5.0). Consequently, the younger goats produced significantly more blastocyst (7.1 +/- 2.7 versus 4.2 +/- 1.4) per head. It was concluded that early pubertal goats treated with E2-P4-FSH could provide a relatively high number of developmentally competent ova for SCNT studies.
authors.affiliation: School of Life Science and Technology, Tongei University, Shanghai 200092, China.
authors.forename: Hong Ying
authors.initials: HY
authors.lastname: Sha
authors.forename: Jian Hua
authors.initials: JH
authors.lastname: Sun
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.forename: Jian Quan
authors.initials: JQ
authors.lastname: Chen
authors.forename: Xu Jun
authors.initials: XJ
authors.lastname: Xu
authors.forename: You Bin
authors.initials: YB
authors.lastname: Wu
authors.forename: Zhi Jun
authors.initials: ZJ
authors.lastname: Huang
authors.forename: Da Wei
authors.initials: DW
authors.lastname: Hu
authors.forename: Guo Ziang
authors.initials: GZ
authors.lastname: Cheng
mesh_terms: D000818:Animals; D048429:Cell Size / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006041:Goats; D009865:Oocytes / Q000166:cytology / Q000187:drug effects*; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects*; D010053:Ovary / Q000166:cytology / Q000187:drug effects; D011374:Progesterone / Q000494:pharmacology; D013997:Time Factors
chemical_list: D011374:Progesterone; D004958:Estradiol; D005640:Follicle Stimulating Hormone