title: A Novel alpha1,2-L-fucosidase acting on xyloglucan oligosaccharides is associated with endo-beta-mannosidase.
abstract: Endo-beta-mannosidase, which hydrolyses the Manbeta1-4GlcNAc linkage of N-glycans in an endo-manner, was discovered in plants. During the course of the purification of the enzyme from lily flowers, we found a higher molecular mass form of the enzyme (designated as EBM II). EBM II was purified by column chromatography to homogeneity and its molecular composition revealed EBM II to be comprised of endo-beta-mannosidase and an associated protein. The cDNA of this associated protein encodes a protein with slight homology to the fucosidase domain of bifidus AfcA. EBM II has alpha1,2-L-fucosidase activity and acts on a fucosylated xyloglucan nonasaccharide. The amino acid sequence of this associated protein has no similarity to known plant alpha-L-fucosidases. These results show that EBM II is a novel alpha1,2-L-fucosidase and a protein complex containing endo-beta-mannosidase.
authors.affiliation: Department of Chemistry, Graduate School of Science, Osaka University, Osaka 560-0043, Japan. txi@chem.sci.osaka-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ishimizu
authors.forename: Chikako
authors.initials: C
authors.lastname: Hashimoto
authors.forename: Ryo
authors.initials: R
authors.lastname: Takeda
authors.forename: Kenta
authors.initials: K
authors.lastname: Fujii
authors.forename: Sumihiro
authors.initials: S
authors.lastname: Hase
mesh_terms: D000595:Amino Acid Sequence; D002240:Carbohydrate Sequence; D003001:Cloning, Molecular; D005936:Glucans / Q000737:chemistry / Q000378:metabolism*; D027762:Lilium / Q000201:enzymology; D008361:Mannosidases / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D013379:Substrate Specificity; D014990:Xylans / Q000737:chemistry / Q000378:metabolism*; D005644:alpha-L-Fucosidase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D005936:Glucans; D009844:Oligosaccharides; D010940:Plant Proteins; D014990:Xylans; C029353:xyloglucan; D008361:Mannosidases; C119276:endo-beta-mannosidase; D005644:alpha-L-Fucosidase