Underlying mechanism of ASIC1a involved in acidosis-induced cytotoxicity in rat C6 glioma cells.
OBJECTIVE
To investigate the underlying mechanism of acid-sensing ion channel (ASIC) 1a involved in the acidosis-induced cytotoxicity of rat C6 glioma cells.


METHODS
The stable ASIC1a-silenced C6 cells built with the RNA interference technology were confirmed by RT-PCR and Western blot analysis. Intracellular calcium ([Ca2+]i) in both the wild-type rat C6 glioma cells and the ASIC1a-silenced C6 cells were analyzed before and after acid application/exposure with the calcium imaging experiment.


RESULTS
The rapid extracellular pH drop induced the increase of [Ca2+]i in the wild-type C6 cells, but not in the ASIC1a-silenced C6 cells. During the prolonged acid exposure, [Ca2+]i was lower in the ASIC1a-silenced C6 cells than that in the control cells.


CONCLUSIONS
The resultant toxicity of [Ca2+]i might contribute to the acidosis-induced cytotoxicity.
Weng
Xie-chuan
XC
Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. wengxc2002@yahoo.com.cn
Zheng
Jian-quan
JQ
Li
Jin
J
Xiao
Wen-bin
WB
D062565:Acid Sensing Ion Channels; D000137:Acid-Base Imbalance / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D005910:Glioma / Q000378:metabolism*; D006851:Hydrochloric Acid / Q000008:administration & dosage / Q000633:toxicity*; D066298:In Vitro Techniques; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D015222:Sodium Channels / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D062565:Acid Sensing Ion Channels; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D015222:Sodium Channels; D006851:Hydrochloric Acid; D002118:Calcium