Characterization of O-linked saccharides on glycoproteins.
Recombinant and native proteins of Pichia pastoris can be O-mannosylated on serine and threonine residues, allowing further elongation reactions to generate short O-linked oligosaccha-rides of mannose. Methods for release from the protein with alkaline beta-elimination with or without reduction of the released saccharides, and for subsequent chromatographic and enzymatic characterization of these saccharides are described.
Bretthauer
Roger K
RK
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, USA.
D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000378:metabolism; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D010843:Pichia / Q000378:metabolism; D013194:Staining and Labeling
D006023:Glycoproteins; D009844:Oligosaccharides; D006026:Glycoside Hydrolases