title: Screening of a microvascular endothelial cDNA library identifies rabaptin 5 as a novel autoantigen in Alzheimer's disease.
abstract: The pathogenesis of Alzheimer's disease (AD) includes the participation of the immune system. To identify antigenic targets in AD, we screened a human microvascular endothelial cell cDNA library with sera from patients with AD, and we identified rabaptin 5 (RABPT5). We detected serum IgG specific to RABPT5 in 65% of patients with AD and in 35% of patients with systemic lupus erythematosus, but in no healthy controls. Our results demonstrated a massive redistribution of this protein in the cytoplasm of endothelial and neuronal cells in apoptosis. In conclusion, we identified RABPT5 as a novel autoantigen in AD.
authors.affiliation: Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Delunardo
authors.forename: Paola
authors.initials: P
authors.lastname: Margutti
authors.forename: Simona
authors.initials: S
authors.lastname: Pontecorvo
authors.forename: Tania
authors.initials: T
authors.lastname: Colasanti
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Conti
authors.forename: Rachele
authors.initials: R
authors.lastname: Riganò
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Profumo
authors.forename: Alessandra
authors.initials: A
authors.lastname: Siracusano
authors.forename: Antonella
authors.initials: A
authors.lastname: Capozzi
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Prencipe
authors.forename: Maurizio
authors.initials: M
authors.lastname: Sorice
authors.forename: Ada
authors.initials: A
authors.lastname: Francia
authors.forename: Elena
authors.initials: E
authors.lastname: Ortona
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000276:immunology*; D000595:Amino Acid Sequence; D001324:Autoantigens / Q000378:metabolism*; D016009:Chi-Square Distribution; D042783:Endothelial Cells / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015723:Gene Library; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D033921:Vesicular Transport Proteins / Q000276:immunology*
chemical_list: D001324:Autoantigens; C097227:RABEP1 protein, human; D033921:Vesicular Transport Proteins