title: Classical swine fever virus inhibits nitric oxide production in infected macrophages.
abstract: Classical swine fever virus (CSFV)-macrophage interactions during infection were analysed by examining macrophage transcriptional responses via microarray. Eleven genes had increased mRNA levels (>2.5-fold, P<0.05) in infected cell cultures, including arginase-1, an inhibitor of nitric oxide production, phosphoinositide 3-kinase, chemokine receptor 4 and interleukin-1beta. Lower levels of nitric oxide and increased arginase activity were found in CSFV-infected macrophages. These changes in gene expression in macrophages suggest viral modulation of host expression to suppress nitric oxide production.
authors.affiliation: Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Zaffuto
authors.affiliation: Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Piccone
authors.affiliation: Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
authors.forename: T G
authors.initials: TG
authors.lastname: Burrage
authors.affiliation: Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
authors.forename: C A
authors.initials: CA
authors.lastname: Balinsky
authors.affiliation: Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
authors.forename: G R
authors.initials: GR
authors.lastname: Risatti
authors.affiliation: Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
authors.forename: M V
authors.initials: MV
authors.lastname: Borca
authors.affiliation: Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
authors.forename: L G
authors.initials: LG
authors.lastname: Holinka
authors.affiliation: Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Rock
authors.affiliation: Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
authors.forename: C L
authors.initials: CL
authors.lastname: Afonso
mesh_terms: D000818:Animals; D001119:Arginase / Q000032:analysis / Q000235:genetics; D002478:Cells, Cultured; D006692:Classical Swine Fever Virus / Q000276:immunology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008264:Macrophages / Q000276:immunology* / Q000821:virology*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D013552:Swine
chemical_list: D012333:RNA, Messenger; D009569:Nitric Oxide; D001119:Arginase