Co-stimulatory molecules on peripheral blood mononuclear cells and tissue infiltrating cells of skin wart and in vitro poke weed mitogen stimulation.
BACKGROUND
Skin wart is a lesion caused by human papilloma viruses (HPVs) that can infect both male and female.


OBJECTIVE
Quantify the number of CD28+, CD86+, CD152+ and gammadelta+ in peripheral blood mononuclear cells (PBMCs) of subjects with skin wart. Identify CD86+ and gammagamma+ cells in skin wart cryosections.


METHODS
Sixteen subjects with skin warts on face, hand, finger, knee, foot or plantar, both male and female, aged between 19-59 years-old, were recruited from Ramathibodi Hospital, Mahidol University, Bangkok.


RESULTS
CD86 and CD152, on peripheral blood mononuclear cells (PBMCs) of subjects with skin wart are significantly lower compared to controls. Tissue cryosection staining for CD86+ and gammadelta+ cells showed no difference among subjects with skin wart and control. Proliferative response to poke weed mitogen of subjects with skin wart is significantly lower than control subjects.


CONCLUSIONS
There was no difference in the number of subjects positive for CD28 and CD86 cell between normal and skin wart subject, but an increase in skin wart subjects with gammadelta+ cells.
Phumeesat
Potjaman
P
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Charuwichitratana
Somyot
S
Vongsakul
Molvibha
M
D000328:Adult; D000938:Antigen-Presenting Cells; D051940:B7-2 Antigen; D016022:Case-Control Studies; D016616:Cryoultramicrotomy; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000276:immunology*; D030361:Papillomavirus Infections; D029604:Phytolacca americana; D014860:Warts / Q000276:immunology / Q000503:physiopathology*
D051940:B7-2 Antigen; D008934:Mitogens