title: Characterization and fine mapping of RppQ, a resistance gene to southern corn rust in maize.
abstract: Southern corn rust (SCR) is a fungal disease caused by Puccinia polysora Underw, which can infect maize and may result in substantial yield losses in maize production. The maize inbred line Qi319 carries the SCR resistance gene RppQ. In order to identify molecular markers linked to the RppQ gene, several techniques were utilized including random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR), and amplified fragment length polymorphism (AFLP). In addition, sequence characterized amplified region (SCAR) techniques combined with bulked segregant analysis (BSA) were used. Seven RAPD markers, eight SSR markers, and sixty-three AFLP primer combinations amplified polymorphisms between two parents and two bulk populations. A large F2 population was used for genetic analysis and for fine mapping of the RppQ gene region. One AFLP polymorphic band, M-CAA/E-AGC 324, was converted to a SCAR marker, MA7, which was mapped to a position 0.46 cM from RppQ. Finally, the RppQ gene was mapped between the SCAR marker MA7 and the AFLP marker M-CCG/E-AGA 157 with distances of 0.46 and 1.71 cM, respectively.
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050016, China.
authors.forename: Chunjiang
authors.initials: C
authors.lastname: Zhou
authors.forename: Cuixia
authors.initials: C
authors.lastname: Chen
authors.forename: Pengxiu
authors.initials: P
authors.lastname: Cao
authors.forename: Suowei
authors.initials: S
authors.lastname: Wu
authors.forename: Jianwei
authors.initials: J
authors.lastname: Sun
authors.forename: Demin
authors.initials: D
authors.lastname: Jin
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D017343:Genes, Plant; D005819:Genetic Markers; D007113:Immunity, Innate; D009181:Mycoses / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
chemical_list: D005819:Genetic Markers