SPR and AFM study of engineered biomolecule immobilisation techniques.
A comparative study into two novel and diverse schemes designed to improve immobilization of biomolecules for biosensing purposes is presented. In the first method a silicon rich matrix is created using PECVD. The second method involves creating nano-patterns on the sensor surface to create a large number of surface discontinuities to which the proteins will bind preferentially. The basic theory of SPR is provided to show the importance of the surface sensitive nature of this optical transduction technique. The present work suggests that both may prove both for SPR and other biosensing applications. Of the two schemes proposed, the results for nano-patterning seem to suggest that it is promoting better surface attachment of biomolecules. The results of SPR and AFM studies are presented that have shown that each of these schemes promotes improved binding of various proteins.
Craig
Ian
I
Nanotechnology Research Institute at the University of Ulster at Jordanstown, BT37 0QB. i.craig@ulster.ac.uk
McLaughlin
James A
JA
D015374:Biosensing Techniques / Q000379:methods*; D018833:Chaperonins / Q000737:chemistry / Q000648:ultrastructure; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000648:ultrastructure; D005353:Fibronectins / Q000737:chemistry / Q000648:ultrastructure; D005949:Glucose Oxidase / Q000737:chemistry / Q000648:ultrastructure; D018625:Microscopy, Atomic Force / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D012825:Silicon / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties
D029968:Escherichia coli Proteins; D005353:Fibronectins; D005949:Glucose Oxidase; D018833:Chaperonins; D012825:Silicon