Nutritional regulation of keratinolytic activity in Bacillus pumilis.
Bacillus pumilis F3-4 utilized feather as a sole source of carbon, nitrogen and sulfur. Supplementation of the feather medium with glucose or MgSO(4) . 7H(2)O increased keratinolytic protease production (14.6-16.7 U/mg). The synthesis of keratinolytic protease was repressed by an exogenous nitrogen source. Keratinolytic protease was produced in the absence of feather (9.4 U/mg). Feather degradation resulted in sulfhydryl group formation (0.8-2.6 microM). B. pumilis F3-4 effectively degraded chicken feather (75%), duck feather (81%) and feather meal (97%), whereas human nails, human hair and sheep wool under went less degradation (9-15%).
Son
Hong-Joo
HJ
School of Applied Life Science, Pusan National University, Miryang, Korea. shjoo@pusan.ac.kr
Park
Hyean-Cheal
HC
Kim
Hong-Sung
HS
Lee
Chung-Yeol
CY
D000818:Animals; D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D002645:Chickens; D003470:Culture Media; D005241:Feathers / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D007633:Keratins / Q000302:isolation & purification / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*
D003470:Culture Media; D007633:Keratins; D010447:Peptide Hydrolases; C035855:keratinase