Comparison of different DNA extraction and polymerase chain reaction methods to detect Leptospira spp. on field samples.
It was carried out a comparison on two reference Leptospira strains DNA between different extraction methods and two polymerase chain reaction protocol. The DNA was quantified and serial dilutions were tested by polymerase chain reaction. The results showed difference in terms of recovery and sensitivity between this methods.
Vitale
Maria
M
Istituto Zooprofilatico Sperimentale della Sicilia A. Mirri, Palermo, Italy. mvitale@pa.izs.it
Vitale
Fabrizio
F
Reale
Stefano
S
Vesco
Gesualdo
G
Currò
Vittoria
V
Caracappa
Santo
S
D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D007558:Italy; D007919:Leptospira / Q000235:genetics* / Q000302:isolation & purification; D041401:Leptospira interrogans serovar pomona / Q000235:genetics / Q000302:isolation & purification; D007922:Leptospirosis / Q000235:genetics / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial