title: Outer membrane protein sequence variation in lambs experimentally infected with Anaplasma phagocytophilum.
abstract: Anaplasma phagocytophilum has long been known to cause tick-borne fever in ruminants and has been identified more recently as the causative agent of the emerging disease human granulocytic anaplasmosis. The related organism Anaplasma marginale uses gene conversion of the expression site for two major outer membrane proteins (OMPs) to generate extensive sequence and antigenic variation in these OMPs. This is thought to present a continuously varying repertoire of epitopes to the mammalian host and allow disease persistence. Recent genomic and structural data on human strains of A. phagocytophilum, together with animal studies in model systems, have implicated an orthologous OMP of A. phagocytophilum in a similar mechanism of variation. However, to date there has been little investigation of the mechanisms of antigenic variation or disease persistence in hosts naturally infected with field strains of A. phagocytophilum. Approximately 300,000 lambs in Norway suffer severe disease caused by A. phagocytophilum annually. We show here the persistent and cyclic nature of infection in these animals that is accompanied by loosely programmed sequence variation of the major OMP expression site in each rickettsemic peak. These data will allow analysis of interactions between A. phagocytophilum and the host immune system in naturally occurring persistent infections and provide an important comparison with enduring infections of cattle caused by A. marginale.
authors.affiliation: Department of Production Animal Clinical Sciences, Section of Small Ruminant Research, Norwegian School of Veterinary Science, Kyrkjevegen 332/334, Sandnes/N-4325, Norway. erik.georg.granquist@veths.no
authors.forename: Erik G
authors.initials: EG
authors.lastname: Granquist
authors.forename: Snorre
authors.initials: S
authors.lastname: Stuen
authors.forename: Anna M
authors.initials: AM
authors.lastname: Lundgren
authors.forename: Margrethe
authors.initials: M
authors.lastname: Bråten
authors.forename: Anthony F
authors.initials: AF
authors.lastname: Barbet
mesh_terms: D000595:Amino Acid Sequence; D041081:Anaplasma phagocytophilum / Q000235:genetics* / Q000502:physiology*; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016873:Ehrlichiosis / Q000382:microbiology / Q000662:veterinary*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D012756:Sheep / Q000382:microbiology*
chemical_list: D001425:Bacterial Outer Membrane Proteins