title: Successful treatment of refractory tibial nonunion using calcium sulphate and bone marrow stromal cell implantation.
abstract: Successful healing of a nine-year tibial nonunion resistant to six previous surgical procedures was achieved by tissue engineering. We used autologous bone marrow stromal cells (BMSCs) expanded to 5 x 10(6) cells after three weeks' tissue culture. Calcium sulphate (CaSO4) in pellet form was combined with these cells at operation. The nonunion was clinically and radiologically healed two months after implantation. This is the description of on healing of a long-standing tibial nonunion by tissue engineering. The successful combination of BMSCs and CaSO4 has not to our knowledge been reported in a clinical setting.
authors.affiliation: Robert Jones and Agnes Hunt, Orthopaedic Hospital, Oswestry, Shropshire SY10 7AG, UK. stefan_bajada@yahoo.com
authors.forename: S
authors.initials: S
authors.lastname: Bajada
authors.forename: P E
authors.initials: PE
authors.lastname: Harrison
authors.forename: B A
authors.initials: BA
authors.lastname: Ashton
authors.forename: V N
authors.initials: VN
authors.lastname: Cassar-Pullicino
authors.forename: N
authors.initials: N
authors.lastname: Ashammakhi
authors.forename: J B
authors.initials: JB
authors.lastname: Richardson
mesh_terms: D000328:Adult; D016026:Bone Marrow Transplantation / Q000379:methods*; D005599:Fractures, Ununited / Q000000981:diagnostic imaging / Q000628:therapy*; D006801:Humans; D008297:Male; D011859:Radiography; D017154:Stromal Cells / Q000637:transplantation*; D013978:Tibial Fractures / Q000000981:diagnostic imaging / Q000628:therapy*; D023822:Tissue Engineering; D014182:Transplantation, Autologous / Q000379:methods; D016896:Treatment Outcome