Useful tips, widely used techniques, and quantifying cell metabolic behavior.
The insect cell culture/baculovirus system has three primary applications: (1) recombinant protein synthesis, (2) biopesticide synthesis, and (3) as a model system (e.g., for studying apoptosis). The fundamental techniques involved in these applications are described throughout this book. In this chapter, the most widely techniques are summarized and the reader is directed to detailed information found elsewhere in this book. Furthermore, many useful tips and the author's personal preferences that are rarely published are discussed in this chapter along with quantitative methods to characterize cell growth, baculovirus infection, and metabolism.
Murhammer
David W
DW
Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, IA, USA.
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002452:Cell Count; D002460:Cell Line; D049109:Cell Proliferation; D007313:Insecta / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics
D011994:Recombinant Proteins