Hindlimb muscle fiber types in two frogs (Rana catesbeiana and Litoria caerulea) with different locomotor behaviors: histochemical and enzymatic comparison.
To test how differences in locomotor behaviors may be reflected in muscle fiber-type diversity within anurans, a comparison of hindlimb muscles between the powerful terrestrial hopper, Rana catesbeiana, and the tree frog, Litoria caerulea, was done. One postural muscle (tibialis posticus, TP) and one primary hopping muscle (plantaris longus, PL), were characterized to identify muscle fiber types using standard histochemical methods. In addition, spectophotometric analysis of activity levels of the oxidative enzyme citrate synthase (CS) and the glycolytic enzyme lactate dehydrogenase (LDH) were done in each muscle. In spite of presumed differences in behavior between the species, we found no significant differences in the proportions of the identified fiber types when the muscles were compared across species. In addition, there were no significant differences in the proportions of the different fiber types between the postural versus phasic muscles within species. Within Rana, the postural muscle (TP) had greater oxidative capacity (as measured by CS activity) than did the phasic muscle (PL). Both muscles had equivalent LDH activities. Within Litoria, PL and TP did not differ in either LDH or CS activities. Both PL and TP of Litoria had less LDH activity and greater CS activity than their homologs in Rana. Thus, in spite of the uniform populations of fiber types between muscles and species, the metabolic diversity based on enzyme activity is consistent with behavioral differences between the species. These results suggest that the range of functional diversity within fiber types may be very broad in anurans, and histochemical fiber typing alone is not a clear indicator of their metabolic or functional properties.
Crockett
Christy J
CJ
Department of Biology, The University of North Carolina at Charlotte, Charlotte, North Carolina 28223, USA.
Peters
Susan E
SE
D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism*; D006614:Hindlimb; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008124:Locomotion; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D018482:Muscle, Skeletal / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D011892:Rana catesbeiana / Q000033:anatomy & histology* / Q000502:physiology; D011898:Ranidae / Q000502:physiology
D007770:L-Lactate Dehydrogenase; D002950:Citrate (si)-Synthase