Evaluation of the insecticidal efficacy of wild-type and recombinant baculoviruses.
A considerable amount of work has been done during the last 20 yr to genetically enhance the efficacy of baculovirus insecticides. Following construction of a genetically altered baculovirus, laboratory bioassays are used to quantify various parameters of insecticidal activity such as the median lethal concentration (or dose) required to kill 50% of infected larvae (LC50 or LD50), median survival time of larvae infected at a fixed dose (ST50), and feeding damage incurred by infected larvae. In this chapter, protocols are described for a variety of bioassays and corresponding data analyses for assessment of the insecticidal activity or host range of baculovirus insecticides. Methods are also provided for baculovirus inoculation of larvae using a microapplicator for determining ST50 or for examining physiological effects.
Li
Huarong
H
Department of Entomology, Iowa State University, Ames, IA, USA.
Bonning
Bryony C
BC
D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001681:Biological Assay / Q000379:methods; D005838:Genotype; D007313:Insecta / Q000166:cytology / Q000235:genetics / Q000821:virology; D007306:Insecticides / Q000378:metabolism*; D007814:Larva / Q000166:cytology / Q000235:genetics / Q000821:virology; D010572:Pest Control, Biological / Q000379:methods*; D011995:Recombination, Genetic
D007306:Insecticides