Simultaneous false-colour imaging of birefringence, extinction and transmittance at camera speed.
A polarized light imaging technique is introduced that simultaneously captures and unfolds transmittance, fast/slow axes directions and birefringence or linear dichroism from single camera exposures. The technique, based on the rotating polarizer method, is capable of sampling an object through polarizers at four different angles in a single image with the use of an image multiplexer. The range of possible applications is demonstrated with examples from the fields of biology, medicine, forensics and material sciences.
Kaminsky
W
W
Department of Chemistry, University of Washington, Seattle, WA 98195, USA. kaminsky@chem.washington.edu
Gunn
E
E
Sours
R
R
Kahr
B
B
D000818:Animals; D001718:Birefringence; D001921:Brain / Q000473:pathology; D002788:Cholesterol Esters / Q000032:analysis / Q000737:chemistry; D003460:Crystallization; D004560:Electricity; D006801:Humans; D007089:Image Enhancement; D008859:Microscopy, Polarization / Q000295:instrumentation / Q000379:methods*; D010710:Phosphates / Q000032:analysis; D010795:Phthalic Acids / Q000737:chemistry / Q000378:metabolism; D058225:Plaque, Amyloid / Q000648:ultrastructure; D005423:Siphonaptera / Q000648:ultrastructure; D012984:Software
D002788:Cholesterol Esters; D010710:Phosphates; D010795:Phthalic Acids; C024788:ammonium phosphate; C032279:phthalic acid; C060123:cholesterol acetate