[Effects of soybean isoflavone on born metabolism and morphology in animal model of osteoporosis rats].
OBJECTIVE
To study the effects of soybean isoflavone (SI) on born metabolism and morphology in animal model of osteoporosis rats.


METHODS
All 70 female Sprague-Dawley (SD) rats were randomly divided into 7 groups according to the levels of total cholesterol (TC) in serum: hyper-lipoid group, estrogen group, low-dose SI group, middle-dose SI group, high-dose SI group, sham group and normal control groups. Bilateral ovaries were extirpated except sham and normal control groups. Except the rats in normal control group, the other rats were fed with high fat diet. Body weight was weighted ad unam vice per week. The estrogen, different dose of SI or deionized water were fed with intragastric administration for 12 weeks. Vena caudalis serum were collected after being ovariectomized, administered for 4 w, 8 w and killed. Serum alkaline phosphatase (AKP) activity and bone density were measured etc.


RESULTS
To interfere of estrogen and SI might recover AKP enzyme activity after its being ovariectomized. There almost sowed no differences between high dose SI intervention and estrogen on bone density and microstructure. Bone loss due to being ovariectomized was relieved after SI intervention. SI might protect cardiocyte myofilament and mitochondrial ultramicrostructure. There was mirror image in estrogen, high dose SI group resembling the normal control group, and there was obvious damage in hyper-lipoids group.


CONCLUSIONS
There should be effects of high dose SI on bone metabolism and morphology in animal model of osteoporosis rats. Serum AKP enzyme activity and bone density should have significantly recovered, the serum level of calcium and phosphorus were maintained after high dose intervened but no significant effects for low dose of SI.
Yu
Qing
Q
Environmental and Public Health School, Wenzhou Medical College, Zhejiang 325035, China.
Su
Yi-xiang
YX
Wang
Wen-wei
WW
Li
An-le
AL
Liu
Cun-li
CL
Wang
Yi-long
YL
Hu
Wan-li
WL
D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D004195:Disease Models, Animal; D005260:Female; D005269:Femur / Q000000981:diagnostic imaging / Q000378:metabolism*; D007529:Isoflavones / Q000494:pharmacology*; D010024:Osteoporosis / Q000378:metabolism* / Q000473:pathology; D010052:Ovariectomy; D011859:Radiography; D051381:Rats; D017207:Rats, Sprague-Dawley; D013025:Glycine max / Q000737:chemistry
D007529:Isoflavones; D000469:Alkaline Phosphatase