title: Genetic diversity of Bartonella henselae in human infection detected with multispacer typing.
abstract: We applied multispacer typing (MST) by incorporating 9 variable intergenic spacers to Bartonella henselae DNA detected in lymph node biopsy specimens from 70 patients with cat-scratch disease (CSD), in cardiac valve specimens from 2 patients with endocarditis, and in 3 human isolates from patients with bacillary angiomatosis, CSD, and endocarditis. Sixteen MST genotypes were found, 5 previously identified in cats and 11 new. Of the studied DNA, 78.7% belonged to 2 genotypes, which were phylogenetically organized into 4 lineages. Human strains were mostly grouped within 2 lineages, previously identified as Marseille and Houston-1. Our results suggest a greater genetic diversity in human-infecting B. henselae than what has previously been evaluated by using other genotyping methods. However, the diversity is not significantly different from that of cat strains. MST is thus a suitable genotyping tool for evaluating the genetic heterogeneity of B. henselae among isolates obtained from human patients.
authors.affiliation: Université de la Méditerraneé, Marseille, France.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Li
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.forename: Pierre-Edouard
authors.initials: PE
authors.lastname: Fournier
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods; D018416:Bartonella henselae / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D002372:Cat-Scratch Disease / Q000382:microbiology*; D002415:Cats; D004269:DNA, Bacterial / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D004269:DNA, Bacterial; D021901:DNA, Intergenic