Surface re-engineering of pancreatic islets with recombinant azido-thrombomodulin.
Chemoselective conjugation of an azido-functionalized thrombomodulin to pancreatic islets was achieved by Staudinger ligation to a surface-bound bifunctional poly(ethylene glycol) linker. The presence of the tethered thrombomodulin resulted in a significant increase in the production of activated protein C with a reduction in islet-mediated thrombogenicity. This report highlights the potential of tissue-targeted chemistry to reduce donor cell mediated procoagulant and proinflammatory responses.
Stabler
Cherie L
CL
Department of Surgery, Emory University, Atlanta, GA 30322, USA.
Sun
Xue-Long
XL
Cui
Wanxing
W
Wilson
John T
JT
Haller
Carolyn A
CA
Chaikof
Elliot L
EL
D000818:Animals; D001386:Azides / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D007515:Islets of Langerhans / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry; D013499:Surface Properties; D018180:Thrombomodulin / Q000737:chemistry*
D001386:Azides; D003432:Cross-Linking Reagents; D018180:Thrombomodulin; D011092:Polyethylene Glycols