The effects of renal ischemia-reperfusion on hemorheological factors: preventive role of allopurinol.
Changes in hemorheological parameters were studied in dogs following unilateral renal artery clamping (45-minute ischemia then reperfusion), with and without preoperative administration of allopurinol. Sham-operated animals were also evaluated. Blood samples were collected preoperatively, at beginning and at 30, 60 and 120 minutes of reperfusion, then on the 1st, 3rd, 5th and 7th days. Filtration properties of erythrocytes (relative cell transit time, RCTT), whole blood and plasma viscosity (WBV, PV), fibrinogen level and hematology parameter were determined. RCTT significantly increased for both ischemic groups at 30 minutes of reperfusion, and remained elevated on the 1st and 2nd postoperative days; these changes were abolished by allopurinol pretreatment. WBV and hematocrit increased on the 1st day, and PV and fibrinogen level showed elevation on 1st-5th postoperative days. We thus conclude that decreases of RBC deformability (i.e., higher RCTT) were characteristic and specific on early postoperative days after renal ischemia-reperfusion and that these alterations were prevented by pre-ischemia administration of allopurinol.
Peto
K
K
Department of Operative Techniques and Surgical Research, Medical and Health Science Centre, Faculty of Medicine, University of Debrecen, Debrecen, Nagyerdei krt. 98, Hungary. kpeto@jaguar.dote.hu
Nemeth
N
N
Brath
E
E
Takacs
I E
IE
Baskurt
O K
OK
Meiselman
H J
HJ
Furka
I
I
Miko
I
I
D000493:Allopurinol / Q000494:pharmacology*; D000818:Animals; D001809:Blood Viscosity / Q000187:drug effects*; D004195:Disease Models, Animal; D004285:Dogs; D004907:Erythrocyte Deformability / Q000187:drug effects*; D004909:Erythrocyte Indices / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology*; D012079:Renal Circulation / Q000187:drug effects; D015427:Reperfusion Injury / Q000097:blood / Q000517:prevention & control*
D016166:Free Radical Scavengers; D000493:Allopurinol