Generation of envelope-modified baculoviruses for gene delivery into mammalian cells.
Genetically modified baculoviruses can efficiently deliver and express genes in mammalian cells. The major prerequisite for the expression of a gene transferred by baculovirus is its control by a promoter that is active in mammalian cells. This chapter describes methods for producing second-generation baculovirus vectors through modification of their envelope. Envelope modified baculoviruses offer additional new applications of the system, such as their use in in vivo gene delivery, targeting, and vaccination. Methods of generating a recombinant baculovirus vector with a modified envelope and its amplification and purification, including technical scale production, are discussed. A variety of notes will give clues as to specific technical procedures. Finally, methods to analyze the virus and transduction procedures are presented.
Hofmann
Christian
C
APIT Laboratories GmbH, Potsdam, Germany.
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D008322:Mammals / Q000235:genetics; D008957:Models, Genetic; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics; D018411:Spodoptera / Q000166:cytology / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics*
D014759:Viral Envelope Proteins