Amperometric immunosensor for the detection of anti-West Nile virus IgG.
An amperometric immunosensor for the detection of West Nile virus (WNV) IgG was developed. This device was based on the immobilization of T7 phages, which were modified by an additional peptide sequence taken from the virus and used as antigen. The electropolymerization of a phage-amphiphilic pyrrole ammonium mixture previously adsorbed on the electrode surface provided an efficient entrapment of phages in a polypyrrole film. After incubation with a secondary peroxidase-labeled antibody, the immunosensors were applied to the quantitative amperometric determination of WNV-antibody at 0 V vs Ag/AgCl via the reduction of the enzymically generated quinone in the presence of hydroquinone and H2O2. The optimum immunosensor configuration detected low WNV-antibody dilutions down to a titer of 1:10(7) with an excellent regeneration of the immunosensor response by glycine treatment.
Ionescu
Rodica E
RE
Département de Chimie Moléculaire UMR-5250, ICMG FR-2607, CNRS Université Joseph Fourier, BP-53, 38041 Grenoble Cedex 9, France.
Cosnier
Serge
S
Herrmann
Sebastien
S
Marks
Robert S
RS
D000478:Alkylation; D017123:Bacteriophage T7 / Q000235:genetics; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012680:Sensitivity and Specificity; D013499:Surface Properties; D014902:West Nile virus / Q000276:immunology*
D007074:Immunoglobulin G; D011108:Polymers; D011758:Pyrroles; D000644:Quaternary Ammonium Compounds; C067635:polypyrrole