title: [Preparative separation of gentiopicrin from Gentiana scabra bunge by high speed counter current chromatography].
abstract: OBJECTIVE
  High speed counter current chromatography was successfully applied to the preparative separation of the component of Gentiana scabra Bunge.
  
  
  METHODS
  Gentiopicrin was obtained by preparative HSCCC with two phase solvent system com-posed of chloroform-methanol-tert-butylmethyl-water (5:4:2:4). The mobile phase is the lower phase and operated at a flow-rate of 2.0 ml/min, while the apparatus rotated at 820 r/min.
  
  
  RESULTS
  High performance liquid chromatogra phic analysis of gentiopicrin was over 96.68%.
  
  
  CONCLUSIONS
  HSCCC is a useful method for the separation of natural products.
authors.affiliation: Institute of Special Wild Economic Animal and Plant Science, C. A. A. S. JiLin 132109, China.
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Wang
authors.forename: Ying-ping
authors.initials: YP
authors.lastname: Wang
authors.forename: Ji-yong
authors.initials: JY
authors.lastname: Liu
authors.forename: Shi
authors.initials: S
authors.lastname: Yan
authors.forename: Cheng-he
authors.initials: CH
authors.lastname: Sun
authors.forename: Shi-quan
authors.initials: SQ
authors.lastname: Xu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D030012:Gentiana / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D000432:Methanol; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry; D012997:Solvents
chemical_list: D005960:Glucosides; D012997:Solvents; D000432:Methanol