title: [Isolation and identification of the mixture of L-hydroxyproline oligo-peptides by reversed-phase high performance liquid chromatography and electrospray ionization tandem mass spectrometry].
abstract: A reversed-phase high performance liquid chromatographic-electrospray ionization tandem mass spectrometric method (RP-HPLC/MS2) was developed for the analysis and identification of a reaction mixture of L-hydroxyproline oligo-peptides. The mixture was separated on a YWG C8 column (10 microm, 250 mm x 10 mm) using acetonitrile-water containing 0.06% trifluoroacetic acid (2:98, v/v) as the mobile phase. The separation conditions were optimized. The mixture was analysed with electrospray ionization mass spectrometry (ESI-MS) and ESI-MS/MS in positive mode. MS and MS2 spectra of individual chromatographic peak were obtained. It indicated that there were hydroxyproline dipeptide, hydroxyproline tripeptide and hydroxyproline cyclo-dipeptide in the mixtures, besides hydroxyproline. The described method can be effectively applied to perform identification of hydroxyproline oligo-peptide mixtures.
authors.affiliation: School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450052, China. sun-yanting@163.com
authors.forename: Yanting
authors.initials: Y
authors.lastname: Sun
authors.forename: Kui
authors.initials: K
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Shuxia
authors.initials: S
authors.lastname: Cao
authors.forename: Yufen
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006909:Hydroxyproline / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry* / Q000302:isolation & purification*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D009842:Oligopeptides; D006909:Hydroxyproline