title: Comparison of multiple-locus variable-number tandem-repeat analysis with other PCR-based methods for typing Brucella suis isolates.
abstract: Multiple-locus variable-number tandem-repeat analysis (MLVA), multiplex PCR, and PCR-restriction fragment length polymorphism analysis were compared for typing Brucella suis isolates. A perfect concordance was obtained among these molecular assays. However, MLVA was the only method to demonstrate brucellosis outbreaks and to confirm that wildlife is a reservoir for zoonotic brucellosis.
authors.affiliation: Departamento de Microbiología y Parasitología, Universidad de Navarra, c/ Irunlarrea no 1, 31008, Pamplona, Spain.
authors.forename: David
authors.initials: D
authors.lastname: García-Yoldi
authors.forename: Philippe
authors.initials: P
authors.lastname: Le Fleche
authors.forename: María J
authors.initials: MJ
authors.lastname: De Miguel
authors.forename: Pilar M
authors.initials: PM
authors.lastname: Muñoz
authors.forename: José M
authors.initials: JM
authors.lastname: Blasco
authors.forename: Zeljko
authors.initials: Z
authors.lastname: Cvetnic
authors.forename: Clara M
authors.initials: CM
authors.lastname: Marín
authors.forename: Gilles
authors.initials: G
authors.lastname: Vergnaud
authors.forename: Ignacio
authors.initials: I
authors.lastname: López-Goñi
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000382:microbiology; D015373:Bacterial Typing Techniques / Q000379:methods*; D041021:Brucella suis / Q000145:classification* / Q000235:genetics*; D002006:Brucellosis / Q000453:epidemiology / Q000382:microbiology*; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D020080:Tandem Repeat Sequences / Q000235:genetics
chemical_list: D004269:DNA, Bacterial