Metabolic engineering of Escherichia coli for 1-butanol production.
Compared to ethanol, butanol offers many advantages as a substitute for gasoline because of higher energy content and higher hydrophobicity. Typically, 1-butanol is produced by Clostridium in a mixed-product fermentation. To facilitate strain improvement for specificity and productivity, we engineered a synthetic pathway in Escherichia coli and demonstrated the production of 1-butanol from this non-native user-friendly host. Alternative genes and competing pathway deletions were evaluated for 1-butanol production. Results show promise for using E. coli for 1-butanol production.
Atsumi
Shota
S
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA 90095, USA.
Cann
Anthony F
AF
Connor
Michael R
MR
Shen
Claire R
CR
Smith
Kevin M
KM
Brynildsen
Mark P
MP
Chou
Katherine J Y
KJ
Hanai
Taizo
T
Liao
James C
JC
D020001:1-Butanol / Q000378:metabolism*; D001674:Bioelectric Energy Sources; D046969:Clostridium acetobutylicum / Q000502:physiology*; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism
D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D020001:1-Butanol