Design, synthesis, and evaluation of peptidyl fluorescent probe for Zn2+ in aqueous solution.
A new fluorescent peptide probe for the detection of Zn(2+) was synthesized on the basis of zinc binding ligands in zinc enzymes. The peptide that has a unique amino acid sequence displayed a great selectivity for Zn(2+) in the presence of several transition metal ions in aqueous solution. The reversibility, binding stoichiometry, binding affinity, and pH sensitivity of the sensor were studied. Further, on-bead application of the peptide as chemosensors was demonstrated.
Joshi
Bishnu Prasad
BP
Department of Chemistry, Bioorganic Chemistry Laboratory, Inha University, Incheon 402-751, Republic of Korea.
Cho
Won-Mi
WM
Kim
Jongseung
J
Yoon
Juyoung
J
Lee
Keun-Hyeung
KH
D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005456:Fluorescent Dyes / Q000032:analysis / Q000138:chemical synthesis*; D010455:Peptides / Q000032:analysis / Q000138:chemical synthesis*; D012996:Solutions / Q000032:analysis* / Q000737:chemistry; D014867:Water / Q000032:analysis* / Q000737:chemistry; D015032:Zinc / Q000032:analysis* / Q000737:chemistry
D005456:Fluorescent Dyes; D010455:Peptides; D012996:Solutions; D014867:Water; D015032:Zinc