[Expressions of beta(3)-adrenoceptor and peroxisome proliferator-activated receptor alpha in human failing heart].
OBJECTIVE
To explore the changes on the content of substrate, the activity of correlative enzyme and the mRNA and protein expressions of beta(3)-adrenoceptor and peroxisome proliferator-activated receptor alpha (PPARalpha) in human failing heart.


METHODS
Papillary muscles from 20 patients with heart failure during mitral valves replacement and 6 control subjects died of non-cardiac accidents were obtained and free fat acid (FFA), lactic acid (LD) and the activity of Na(+)K(+)-ATPase and Ca(2+)Mg(2+)-ATPase, protein and mRNA expressions of beta(3)-adrenergic receptor and PPARalpha were measured.


RESULTS
In the failing heart, the contents of fat acid, LD and expression of beta(3)-adrenoceptor mRNA and protein were significantly higher while the activity of Na(+)K(+)-ATPase and Ca(2+)Mg(2+)-ATPase, expressions of PPARalpha at mRNA and protein levels were significantly lower than those in control myocardium.


CONCLUSIONS
Metabolic remodeling (upregulation of beta(3)-adrenoceptor and downregulation of PPARalpha) might contribute to the pathophysiology of heart failure.
Tian
Ying
Y
Department of Cardiology, Anzhen Hospital, Capital University of Medical Sciences, Beijing 100029, China.
Zhu
Shan-jun
SJ
Wang
Jiang
J
Li
Zhen-kui
ZK
Song
Rong
R
Nie
Ling
L
D000328:Adult; D005260:Female; D006333:Heart Failure / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism*; D047493:PPAR alpha / Q000378:metabolism*; D022702:Receptors, Adrenergic, beta-3 / Q000378:metabolism*
D047493:PPAR alpha; D022702:Receptors, Adrenergic, beta-3