Efficient nuclear delivery and nuclear body localization of antisense oligo-nucleotides using degradable polymersomes.
Delivery of antisense oligonucleotides, AON, presents many of the same challenges as delivery of any nucleic acid: charge, stability, cell uptake, endolysosomal escape, and entry into the nucleus. Here we demonstrate efficient delivery of AON after loading into biodegradable polymer vesicles or 'polymersomes'. We focus on AON delivery to muscle cells in vitro and in vivo because of the emergence of AON in therapeutic strategies directed at muscular dystrophies. To first clarify uptake kinetics without the complications of typical multi-layered myotube cultures, we use micro-patterned C2C12 cells and show efficient uptake of AON-polymersomes. The biodegradable polymersomes break down and foster AON escape with the binding of fluorescent-AON into the nuclear bodies. Intramuscular injections of the polymersome-AON into the hind limbs of mdx-dystrophic mice show more efficient nuclear uptake than AON alone and also lead to dystrophin expression in the mdx mice. In sum, these neutral, degradable carriers of AON show promise in vivo.
Kim
Younghoon
Y
Chem. & Biomolecular Eng. Dept., Pennsylvania Univ., Philadelphia, PA 19104, USA.
Tewari
Manu
M
Pajeroski
David J
DJ
Sen
Shamik
S
Jason
Williams
W
Sirsi
Shashank
S
Lutz
Gordon
G
Discher
Dennis E
DE
D000818:Animals; D001672:Biocompatible Materials; D001673:Biodegradation, Environmental; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D016503:Drug Delivery Systems; D016189:Dystrophin / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D018101:Mice, Inbred mdx; D016376:Oligonucleotides, Antisense / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013997:Time Factors
D001672:Biocompatible Materials; D016189:Dystrophin; D016376:Oligonucleotides, Antisense; D011108:Polymers