Alternative bioreactor strategy for probing infection and production.
Probing the baculovirus infection process is essential in optimizing the recombinant protein production. Typically, researchers monitor the infection process in the stirred tank reactor, which, however, contains a population of cells infected at different times after virus inoculation. This chapter describes a two-stage reactor system consisting of an upstream continuous stirred tank reactor and a downstream tubular reactor with segmented plug flow for probing baculovirus infection and production.
Hu
Yu-Chen
YC
Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
Bentley
William E
WE
D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D019149:Bioreactors; D002460:Cell Line; D018411:Spodoptera / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism
D001616:beta-Galactosidase