Regulation of adipocyte differentiation of bone marrow stromal cells by transcription factor GATA-2.
GATA-2 is a transcription factor expressed in stem and progenitor cells of various cell lineages. In this study, we examined the role of GATA-2 in the differentiation of adipocytes, which are the main components of the microenvironment for hematopoiesis. Suppression of GATA-2 in TBR343, a preadipocytic stromal cell line, resulted in the acceleration of adipocyte differentiation. Conversely, overexpression of GATA-2 induced the resistance of adipocyte differentiation. In addition, regulatory elements of the GATA-2 gene in TBR343 are the same as those in hematopoietic cells. These results suggest that GATA-2 maintains a hematopoietic microenvironment by regulating the differentiation of adipocytes.
Okitsu
Yoko
Y
Department of Hematology and Rheumatology, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan.
Takahashi
Shinichiro
S
Minegishi
Naoko
N
Kameoka
Junichi
J
Kaku
Mitsuo
M
Yamamoto
Masayuki
M
Sasaki
Takeshi
T
Harigae
Hideo
H
D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D050989:GATA2 Transcription Factor / Q000502:physiology*; D006410:Hematopoiesis; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism
D050989:GATA2 Transcription Factor; C494712:Gata2 protein, mouse