A Golgi fragmentation pathway in neurodegeneration.
The Golgi apparatus processes intracellular proteins, but undergoes disassembly and fragmentation during apoptosis in several neurodegenerative disorders such as amyotrophic lateral sclerosis and Alzheimer's disease. It is well known that other cytoplasmic organelles play important roles in cell death pathways. Thus, we hypothesized that Golgi fragmentation might participate in transduction of cell death signals. Here, we found that Golgi fragmentation and dispersal precede neuronal cell death triggered by excitotoxins, oxidative/nitrosative insults, or ER stress. Pharmacological intervention or overexpression of the C-terminal fragment of Grasp65, a Golgi-associated protein, inhibits fragmentation and decreases or delays neuronal cell death. Inhibition of mitochondrial or ER cell death pathways also decreases Golgi fragmentation, indicating crosstalk between organelles and suggesting that the Golgi may be a common downstream-effector of cell death. Taken together, these findings implicate the Golgi as a sensor of stress signals in cell death pathways.
Nakagomi
Saya
S
Center for Neuroscience, Aging, and Stem Cell Research, Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.
Barsoum
Mark J
MJ
Bossy-Wetzel
Ella
E
Sütterlin
Christine
C
Malhotra
Vivek
V
Lipton
Stuart A
SA
D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000473:pathology; D003545:Cysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D006056:Golgi Apparatus / Q000187:drug effects / Q000473:pathology*; D008164:Luminescent Proteins / Q000096:biosynthesis; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D016202:N-Methylaspartate / Q000494:pharmacology; D009410:Nerve Degeneration / Q000139:chemically induced / Q000235:genetics / Q000473:pathology*; D009474:Neurons / Q000187:drug effects / Q000473:pathology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D051381:Rats; D026403:S-Nitrosothiols / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors; D014162:Transfection / Q000379:methods; D014404:Tubulin / Q000378:metabolism
D018690:Excitatory Amino Acid Agonists; D008164:Luminescent Proteins; D020030:Nitric Oxide Donors; D026403:S-Nitrosothiols; D014404:Tubulin; D016202:N-Methylaspartate; C021315:S-nitrosocysteine; D003545:Cysteine