title: [Fluorescence characteristics and photophysical parameters of aggregates of light-harvesting chlorophyll a/b complexes].
abstract: Changes in the fluorescence characteristics and molecular photophysical parameters induced by disaggregation of light-harvesting chlorophyll a/b complexes isolated from pea were studied. Disaggregation was achieved by increasing the concentration of the detergent triton X-100 (concentration range 0-230 microM, chlorophyll concentration 2.45 microg/ml). Laser fluorimetry methods were used to measure the molecular photophysical parameters. It was shown that the decrease in aggregate size is accompanied by a decrease in fluorescence at 700 nm at 77 K, a fall of the singlet-singlet annihilation rate (by more than two orders), and the growth of fluorescence life time (from 160 ps to 3.2 ns).
authors.forename: E E
authors.initials: EE
authors.lastname: Ostroumov
authors.forename: V V
authors.initials: VV
authors.lastname: Fadeev
authors.forename: M S
authors.initials: MS
authors.lastname: Khristin
authors.forename: V Z
authors.initials: VZ
authors.lastname: Pashchenko
authors.forename: V B
authors.initials: VB
authors.lastname: Tusov
mesh_terms: D002734:Chlorophyll / Q000737:chemistry*; D000077194:Chlorophyll A; D003080:Cold Temperature; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry*; D017830:Octoxynol / Q000737:chemistry*; D018532:Pisum sativum / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D045342:Light-Harvesting Protein Complexes; D010940:Plant Proteins; D002734:Chlorophyll; C037184:chlorophyll b; D017830:Octoxynol; D000077194:Chlorophyll A