The induction of heme oxygenase-1 modulates bismuth oxide-induced cytotoxicity in human dental pulp cells.
The aim of this study was to investigate the cytotoxic and nitric oxide (NO)-inducing effects of bismuth oxide (Bi(2)O(3))-containing Portland cement (BPC) on human dental pulp cells. We also assessed whether heme oxygenase-1 (HO-1) is involved in BPC-induced cytotoxicity in dental pulp cells. Cytotoxicity and NO production induced by BPC were higher than those induced by Portland cement at 12 and 24 hours, and the former gradually decreased to the level observed for PC. HO-1 and inducible nitric oxide synthase messenger RNA expressions in the BPC group showed maximal increase at 24 hours, and it gradually decreased with increasing cultivation time. Hemin treatment reversed the BPC-induced cytotoxicity, whereas zinc protoporphyrin IX treatment increased the cytotoxicity. These results suggested that NO production by BPC correlates with HO-1 expression in dental pulp cells. Moreover, BPC-induced HO-1 expression in dental pulp cells plays a protective role against the cytotoxic effects of BPC.
Min
Kyung-San
KS
Department of Conservative Dentistry, College of Dentistry, Wonkwang University, Iksan, South Korea.
Chang
Hoon-Sang
HS
Bae
Ji-Myung
JM
Park
Sang-Hyuk
SH
Hong
Chan-Ui
CU
Kim
Eun-Cheol
EC
D001729:Bismuth / Q000633:toxicity*; D002478:Cells, Cultured; D003738:Dental Cements / Q000633:toxicity; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D003785:Dental Pulp Capping; D051547:Heme Oxygenase-1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D006427:Hemin / Q000494:pharmacology; D006801:Humans; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D011524:Protoporphyrins / Q000494:pharmacology; D012387:Root Canal Filling Materials / Q000633:toxicity*
D003738:Dental Cements; D011524:Protoporphyrins; D012387:Root Canal Filling Materials; C472247:accelerated Portland cement; C017803:zinc protoporphyrin; D009569:Nitric Oxide; D006427:Hemin; C033301:bismuth oxide; D052247:Nitric Oxide Synthase Type II; D051547:Heme Oxygenase-1; D001729:Bismuth