[Effects of peroxisome proliferators activated receptors on caveolin-1 expression in foam cells].
OBJECTIVE
To study the effect of peroxisome proliferators activated receptors (PPAR) alpha, gamma ligand on ATP-binding cassette transporter A1 (ABCA1) and caveolin-1 expressions and cholesterol, ox-LDL contents in human monocyte derived foam cells.


METHODS
Malondialdehyde (MDA) was measured by TBARS method, ox-LDL detected by ELISA method, cholesterol measured by fluorescence spectrophotometric method, ABCA1, caveolin-1 mRNA and protein expressions determined by RT-PCR and Western blot, in human monocytes, foam cells [human monocyte-derived macrophage induced by myristate acetate (PMA) further treated with 50 mg/L ox-LDL for 24 h], foam cells plus 10 micromol/L pioglitazone for 48 h, foam cells plus 5 micromol/L clofibrate for 48 h.


RESULTS
The intracellular total cholesterol (TC), free cholesterol (FC), cholesteryl ester (CE), ox-LDL and lipid peroxide were significantly increased and the membrane expressions of ABCA1, caveolin-1 were down-regulated in foam cells compared to monocytes (all P < 0.05) and these changes were significantly attenuated by cotreatment with PPARalpha, gamma ligand.


CONCLUSIONS
The anti-atherosclerosis effects of PPARalpha, gamma ligand are related to reducing cholesterol contents and up-regulating ABCA1, caveolin-1 expressions in foam cells.
Jiang
Yi-deng
YD
Department of Pathophysiology, Ningxia Medical College, Yinchuan 750004, China. jwcjyd@163.com
Xiong
Jian-tuan
JT
Cao
Jun
J
Li
Gui-zhong
GZ
Wang
Shu-ren
SR
D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D051242:Caveolin 1 / Q000378:metabolism*; D002460:Cell Line; D002784:Cholesterol / Q000235:genetics / Q000378:metabolism*; D005487:Foam Cells / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism; D047493:PPAR alpha / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism*
C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D051242:Caveolin 1; D047493:PPAR alpha; D047495:PPAR gamma; D008315:Malondialdehyde; D002784:Cholesterol