title: Muju virus, a novel hantavirus harboured by the arvicolid rodent Myodes regulus in Korea.
abstract: Acute-phase sera from >5 % of cases of haemorrhagic fever with renal syndrome occurring annually in Korea have been found to exhibit a fourfold or higher antibody titre to Puumala virus (PUUV) than to Hantaan virus (HTNV) by double-sandwich IgM ELISA, suggesting the existence of a PUUV-related hantavirus. Based on the phylogenetic relationships among arvicolid rodents, the royal vole (Myodes regulus) was targeted as a likely reservoir host of hantavirus. Using RT-PCR, a genetically distinct hantavirus, designated Muju virus (MUJV), was detected in lung tissue of royal voles, captured in widely separated geographical regions in Korea during 1996-2007. Pairwise analysis of the full-length S (1857 nt) and M (3634 nt) segments of MUJV indicated approximately 77 % sequence similarity with PUUV. At the amino acid level, MUJV differed from PUUV by 5.5-6.9 % (nucleocapsid) and 10.0-11.6 % (Gn and Gc envelope glycoproteins). Interstrain variation of MUJV sequences from royal voles captured in different regions suggested geographic-specific clustering. Neutralizing antibody titres against PUUV were two- to sixfold higher than to HTNV in sera of MUJV-infected Myodes regulus. Although virus isolation attempts were unsuccessful, the collective data indicate that MUJV is a distinct hantavirus species.
authors.affiliation: Bank for Pathogenic Viruses, Korea University, Seoul, Korea.|Department of Microbiology, College of Medicine, Korea University, Seoul, Korea.
authors.forename: Ki-Joon
authors.initials: KJ
authors.lastname: Song
authors.affiliation: Bank for Pathogenic Viruses, Korea University, Seoul, Korea.|Department of Microbiology, College of Medicine, Korea University, Seoul, Korea.
authors.forename: Luck Ju
authors.initials: LJ
authors.lastname: Baek
authors.affiliation: Department of Microbiology, College of Medicine, Korea University, Seoul, Korea.
authors.forename: Sungsil
authors.initials: S
authors.lastname: Moon
authors.affiliation: Department of Microbiology, College of Medicine, Korea University, Seoul, Korea.
authors.forename: Si Jung
authors.initials: SJ
authors.lastname: Ha
authors.affiliation: Department of Microbiology, College of Medicine, Korea University, Seoul, Korea.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Bank for Pathogenic Viruses, Korea University, Seoul, Korea.|Department of Microbiology, College of Medicine, Korea University, Seoul, Korea.
authors.forename: Kwang Sook
authors.initials: KS
authors.lastname: Park
authors.affiliation: Force Health Protection, 18th Medical Command, Unit 15281, APO AP 96205, Korea.
authors.forename: Terry A
authors.initials: TA
authors.lastname: Klein
authors.affiliation: Force Health Protection, 18th Medical Command, Unit 15281, APO AP 96205, Korea.
authors.forename: William
authors.initials: W
authors.lastname: Sames
authors.affiliation: 5th Medical Detachment, 168th Medical Battalion (Area Support), 18th Medical Command, Unit 15247, APO AP 96205, Korea.
authors.forename: Heung-Chul
authors.initials: HC
authors.lastname: Kim
authors.affiliation: Virology Division, US Army Medical Research Institute of Infectious Diseases, Frederick, MD, USA.
authors.forename: John S
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Pediatrics, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Yanagihara
authors.affiliation: Bank for Pathogenic Viruses, Korea University, Seoul, Korea.|Department of Microbiology, College of Medicine, Korea University, Seoul, Korea.
authors.forename: Jin-Won
authors.initials: JW
authors.lastname: Song
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000821:virology*; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D006476:Orthohantavirus / Q000145:classification* / Q000302:isolation & purification*; D018778:Hantavirus Infections / Q000662:veterinary*; D007723:Korea; D008168:Lung / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D014764:Viral Proteins