Magnesium ion is an effective inhibitor of the binding of Escherichia coli to heparin.
We investigated the effects of ions and temperature on the binding of E. coli to heparin using a chemiluminescence electrophoretic mobility shift assay. We found that magnesium ion is an effective inhibitor of the binding. The method can be readily applied to discover agents that can block the binding.
Kang
Jonghoon
J
Sealy Center for Structural Biology and Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, Texas 77555-1157, USA. jkang@vbi.vt.edu
Lee
Myung Soog
MS
Gorenstein
David G
DG
D024202:Electrophoretic Mobility Shift Assay / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D006493:Heparin / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D008274:Magnesium / Q000737:chemistry*; D013696:Temperature
D006493:Heparin; D008274:Magnesium