title: Evaluation of a method for measuring tissue non-specific alkaline phosphatase activity in healthy subjects.
abstract: BACKGROUND
  Intestinal alkaline phosphatase (IAP) isozymes in the healthy human serum samples appears in two isoforms: normal-molecular-weight IAP (NIAP) and high-molecular-weight IAP (HIAP). We have demonstrated that the reference range for serum alkaline phosphatase (ALP) activity is higher in blood group B and O antigen secretors than in the tested other blood groups, for the appearance of these isoforms is depended on blood group B or O antigen secretors.
  
  
  METHODS
  We assessed a diethanolamine-L-phenylalanine (DEA-Phe) method for measuring tissue non-specific alkaline phosphatase (TNAP). This assays the sum of liver alkaline phosphatase and bone alkaline phosphatase activities as determined by an inhibiting IAP activity method with Phe. We classified 420 healthy subjects into two groups, a group of subjects who had blood group B or O antigen secretors (n = 184) and a group of subjects who had other blood groups (n = 236).
  
  
  RESULTS
  ALP activity was higher in the B or O secretor group than in the other group: 20.9% higher (P<0.001) by the N-methyl-D-glucamine method, 13.7% higher (P<0.002) by 2-amino-2-methyl-1-propanol method, and 9.6% higher (P<0.05) by the diethanolamine method, but there was no significant difference in TNAP activity between the two blood group when measured by the DEA-Phe method.
  
  
  CONCLUSIONS
  These results of this study support the expectation that the DEA-Phe method would be specific for TNAP activity. In addition, the reference range for TNAP activity did not vary with the differences in the tested all blood groups.
authors.affiliation: Department of Clinical Chemistry, Saitama Prefectural University, 820 Sannomiya, Koshigaya 343-8540, Japan. matsushita-m@spu.ac.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsushita
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Irino
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kamiyama
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Muramoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kawaguchi
authors.forename: Takanari
authors.initials: T
authors.lastname: Nakano
authors.forename: Tsugikazu
authors.initials: T
authors.lastname: Komoda
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000032:analysis / Q000097:blood / Q000378:metabolism; D001789:Blood Group Antigens / Q000097:blood; D004796:Clinical Enzyme Tests / Q000379:methods*; D004983:Ethanolamines / Q000032:analysis / Q000737:chemistry; D058851:GPI-Linked Proteins; D006262:Health; D006801:Humans; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D001789:Blood Group Antigens; D004983:Ethanolamines; D058851:GPI-Linked Proteins; D007527:Isoenzymes; C020283:diethanolamine; C063088:ALPI protein, human; D000469:Alkaline Phosphatase