Isolation and identification of antiplatelet aggregatory principles from the leaves of Piper lolot.
The methanolic extract of Piper lolot, having shown potent inhibitory activity on platelet aggregation induced by arachidonic acid (AA) and platelet activating factor (PAF), was subjected to activity-guided isolation to yield twelve new amide alkaloids, piperlotine A-L (1-12), along with twenty-nine known compounds. Their structures were elucidated on the basis of spectroscopic analysis. The isolated compounds were tested for their inhibitory activity on the rabbit platelet aggregation. The compounds piperlotine A (1), piperlotine C (3), piperlotine D (4), piperlotine E (5), 3-phenyl-1-(2,4,6-trihydroxyphenyl)propan-1-one (21), 3-(4-methoxyphenyl)-1-(2,4,6-trihydroxyphenyl)propan-1-one (22), 1-trans-cinnamoylpyrrolidine (24), sarmentine (26), pellitorine (27), methyl 3-phenylpropionate (32), and (10S)-10-hydroxypheophorbide a methyl ester (40) showed potent antiplatelet aggregation activity.
Li
Chia-Ying
CY
Department of Chemistry, National Cheng Kung University, Tainan, Taiwan.
Tsai
Wei-Jern
WJ
Damu
Amooru Gangaiah
AG
Lee
E-Jian
EJ
Wu
Tian-Shung
TS
Dung
Nguyen Xuan
NX
Thang
Tran Dinh
TD
Thanh
Le
L
D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D031701:Piper / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D011817:Rabbits
D000470:Alkaloids; D010975:Platelet Aggregation Inhibitors