Effect of propofol on mucous permeability and inflammatory mediators expression in the intestine following traumatic brain injury in rats.
OBJECTIVE
To explore whether propofol could in vivo, suppress intestinal permeability and proinflammatory cytokines in the intestine after traumatic brain injury (TBI).


METHODS
The rats were randomly divided into four groups including control group with right parietal bone window alone and no brain injury, TBI group, TBI+propofol (5mg/kg/h), and propofol alone group. All animals were killed 72h later. Plasma endotoxin was assayed by the chromogenic limulus amebocyte lysate test. Intestinal permeability was quantified using the lactulose/mannitol (L/M) test. TNF-alpha and IL-6 in the intestine were measured using commercially available enzyme-linked immunoassay Kits. Nuclear factor kappa B (NF-kappaB) was analyzed by Electrophoretic mobility shift assay (EMSA).


RESULTS
No rats died before sacrifice. Plasma endotoxin, L/M ratio, intestinal level of NF-kappaB, TNF-alpha, and IL-6 remained at baseline level in controlled animals. TBI promoted a significant increment in plasma endotoxin, L/M ratio, intestinal level of NF-kappaB, TNF-alpha, and IL-6. Propofol inhibited those parameters at the dose of 5mg/kg/h.


CONCLUSIONS
Propofol has the protective effect on the intestine following traumatic brain injury in rats.
Sun
Jie
J
Department of Anesthesiology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, PR China.
Wang
Lin
L
Shen
Jinchun
J
Wang
Zhongyun
Z
Qian
Yanning
Y
D000818:Animals; D001930:Brain Injuries / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D004731:Endotoxins / Q000097:blood*; D005786:Gene Expression Regulation / Q000187:drug effects; D006993:Hypnotics and Sedatives / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000378:metabolism*; D015850:Interleukin-6 / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D008297:Male; D016328:NF-kappa B / Q000096:biosynthesis; D010539:Permeability / Q000187:drug effects; D015742:Propofol / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D004731:Endotoxins; D006993:Hypnotics and Sedatives; D018836:Inflammation Mediators; D015850:Interleukin-6; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D015742:Propofol