title: Sex chromosome complement affects nociception in tests of acute and chronic exposure to morphine in mice.
abstract: We tested the role of sex chromosome complement and gonadal hormones in sex differences in several different paradigms measuring nociception and opioid analgesia using "four core genotypes" C57BL/6J mice. The genotypes include XX and XY gonadal males, and XX and XY gonadal females. Adult mice were gonadectomized and tested 3-4 weeks later, so that differences between sexes (mice with testes vs. ovaries) were attributable mainly to organizational effects of gonadal hormones, whereas differences between XX and XY mice were attributable to their complement of sex chromosomes. In Experiment 1 (hotplate test of acute morphine analgesia), XX mice of both gonadal sexes had significantly shorter hotplate baseline latencies prior to morphine than XY mice. In Experiment 2 (test of development of tolerance to morphine), mice were injected twice daily with 10 mg/kg morphine or saline for 6 days. Saline or the competitive NMDA antagonist CPP (3-(2-carboxypiperazin-4yl) propyl-1-phosphonic acid) (10 mg/kg) was co-injected. On day 7, mice were tested for hotplate latencies before and after administration of a challenge dose of morphine (10 mg/kg). XX mice showed shorter hotplate latencies than XY mice at baseline, and the XX-XY difference was greater following morphine. In Experiment 3, mice were injected with morphine (10 mg/kg) or saline, 15 min before intraplantar injection of formalin (5%/25 microl). XX mice licked their hindpaw more than XY mice within 5 min of formalin injection. The results indicate that X- or Y-linked genes have direct effects, not mediated by gonadal secretions, on sex differences in two different types of acute nociception.
authors.affiliation: Department of Physiological Science, and Laboratory of Neuroendocrinology of the Brain Research Institute, University of California, Los Angeles, CA 90095, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Gioiosa
authors.forename: Xuqi
authors.initials: X
authors.lastname: Chen
authors.forename: Rebecca
authors.initials: R
authors.lastname: Watkins
authors.forename: Nicole
authors.initials: N
authors.lastname: Klanfer
authors.forename: Camron D
authors.initials: CD
authors.lastname: Bryant
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Evans
authors.forename: Arthur P
authors.initials: AP
authors.lastname: Arnold
mesh_terms: D000701:Analgesics, Opioid / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D004361:Drug Tolerance / Q000235:genetics / Q000502:physiology; D005260:Female; D024282:Genes, sry / Q000235:genetics; D005838:Genotype; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009020:Morphine / Q000494:pharmacology*; D009619:Nociceptors / Q000187:drug effects / Q000502:physiology; D017288:Pain Threshold / Q000187:drug effects / Q000502:physiology*; D010465:Perception / Q000187:drug effects / Q000502:physiology; D011930:Reaction Time / Q000502:physiology; D012727:Sex Characteristics; D012730:Sex Chromosomes; D019849:Sex Determination Processes; D012737:Sex Factors
chemical_list: D000701:Analgesics, Opioid; D009020:Morphine