Decursin and decursinol angelate selectively inhibit NADH-fumarate reductase of Ascaris suum.
NADH-fumarate reductase (NFRD) is a key enzyme in many anaerobic helminths. Decursin and decursinol angelate have been isolated from the roots of ANGELICA GIGAS Nakai (Apiaceae) as NFRD inhibitors. They inhibited ASCARIS SUUM NFRD with IC (50) values of 1.1 and 2.7 microM, respectively. Their target is the electron transport enzyme complex I. Since the inhibitory activities of decursin against bovine heart complexes are weak, it is a selective inhibitor of the nematode complex I. In contrast, decursinol angelate moderately inhibits bovine heart complexes II and III. Decursinol inhibits A. SUUM NFRD to a similar extent, but its target is complex II. It also inhibits bovine heart complexes II and III.
Shiomi
Kazuro
K
Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan. shiomi@lisci.kitasato-u.ac.jp
Hatano
Hiroko
H
Morimoto
Hiromi
H
Ui
Hideaki
H
Sakamoto
Kimitoshi
K
Kita
Kiyoshi
K
Tomoda
Hiroshi
H
Lee
Eun Woo
EW
Heo
Tae Ryeon
TR
Kawagishi
Hirokazu
H
Omura
Satoshi
S
D029969:Angelica / Q000737:chemistry; D000818:Animals; D000871:Anthelmintics / Q000737:chemistry / Q000494:pharmacology*; D017165:Ascaris suum / Q000201:enzymology*; D001578:Benzopyrans / Q000737:chemistry / Q000494:pharmacology*; D002087:Butyrates / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000037:antagonists & inhibitors*
D000871:Anthelmintics; D001578:Benzopyrans; D002087:Butyrates; C101278:decursin; D044925:Oxidoreductases Acting on CH-CH Group Donors; C020155:fumarate reductase (NADH)