title: Isolation of a cyprinid herpesvirus 2 from goldfish, Carassius auratus (L.), in the UK.
abstract: Haematopoietic necrosis virus [cyprinid herpesvirus 2 (CyHV-2)] was isolated during disease outbreaks in goldfish, Carassius auratus, at an ornamental fish retail site in southern England in 2004. Signs of disease included lethargy and inappetence and were first seen after water temperatures increased from 14-15 to 19-21 degrees C. External gross pathology included pale patches on the gills and skin and internally the spleen was enlarged, often with distinctive white nodules. The most prominent histopathological changes observed were necrotic lesions in the spleen and kidney and focal patches of necrosis in the gill lamellae. Necrotic cells often contained nuclei with marginated chromatin and pale intranuclear inclusions. Ultrastructural examination of the spleen tissue revealed typical herpesvirus-like particles measuring 100 nm in diameter. The virus was isolated from extracts of gill tissue in KF-1 cells at 20 degrees C and oligonucleotide primer sets were designed based on conserved gene sequences and used to amplify viral DNA by polymerase chain reaction (PCR). The PCR assays were then used to detect the virus in DNA extracted from tissues sampled during earlier disease investigations at the retail site owner's holding facility in 2002 and 2003 and stored at -70 degrees C since then. Polymerase gene-specific PCR amplification products obtained from tissue samples and from the virus isolated in cell culture shared 100% nucleotide sequence identity with the published sequence for CyHV-2.
authors.affiliation: Centre for Environment, Fisheries and Aquaculture Science, Weymouth Laboratory, The Nothe, Weymouth, Dorset, UK.
authors.forename: K R
authors.initials: KR
authors.lastname: Jeffery
authors.forename: K
authors.initials: K
authors.lastname: Bateman
authors.forename: A
authors.initials: A
authors.lastname: Bayley
authors.forename: S W
authors.initials: SW
authors.lastname: Feist
authors.forename: J
authors.initials: J
authors.lastname: Hulland
authors.forename: C
authors.initials: C
authors.lastname: Longshaw
authors.forename: D
authors.initials: D
authors.lastname: Stone
authors.forename: G
authors.initials: G
authors.lastname: Woolford
authors.forename: K
authors.initials: K
authors.lastname: Way
mesh_terms: D000818:Animals; D002460:Cell Line; D003530:Cyprinidae / Q000821:virology; D017931:DNA Primers / Q000737:chemistry; D005393:Fish Diseases / Q000473:pathology / Q000821:virology*; D005880:Gills / Q000473:pathology; D006054:Goldfish / Q000821:virology*; D006564:Herpesviridae / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D007668:Kidney / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013154:Spleen / Q000473:pathology; D006113:United Kingdom
chemical_list: D017931:DNA Primers