title: Detection of high-risk papillomavirus DNA with commercial invader-technology-based analyte-specific reagents following automated extraction of DNA from cervical brushings in ThinPrep media.
abstract: We compared the performances of the Third Wave Technology Invader method and the Digene Hybrid Capture 2 assay to detect high-risk human papillomaviruses in 87 cervical brushing specimens submitted in Cytyc ThinPrep media. Two different methods for the extraction of DNA from squamous epithelial cells were also evaluated.
authors.affiliation: CompuNet Clinical Laboratories, Moraine, OH, USA. ted.e.schutzbank@questdiagnostics.com
authors.forename: Ted E
authors.initials: TE
authors.lastname: Schutzbank
authors.forename: Charlene
authors.initials: C
authors.lastname: Jarvis
authors.forename: Nicole
authors.initials: N
authors.lastname: Kahmann
authors.forename: Katherine
authors.initials: K
authors.lastname: Lopez
authors.forename: Marlea
authors.initials: M
authors.lastname: Weimer
authors.forename: Aleta
authors.initials: A
authors.lastname: Yount
mesh_terms: D002584:Cervix Uteri / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000821:virology*; D011933:Reagent Kits, Diagnostic
chemical_list: D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic