Image-based high-throughput quantification of cellular fat accumulation.
A number of biochemical methods are available for measuring fat accumulation in cell culture. The authors report a simple image-based method for measuring fat accumulation in adipocytes using a combination of high-throughput brightfield microscopy and image analysis, which was validated biochemically using Oil-Red-O. The quickest and most accurate method of analysis was one based on thresholding brightfield images and determining the area of fat droplets per image. Thus, the authors have developed a simple high-throughput, label-free method for measuring fat accumulation that is applicable to any cell or tissue type where fat droplets are visible under light microscopy.
Dragunow
Mike
M
Department of Pharmacology, Faculty of Medical and Health Sciences, University of Auckland, New Zealand. m.dragunow@auckland.ac.nz
Cameron
Rachel
R
Narayan
Pritika
P
O'Carroll
Simon
S
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism; D000818:Animals; D005223:Fats / Q000378:metabolism*; D051379:Mice
D005223:Fats