title: The extent of periodontal disease and the IL-6 -174 genotype as determinants of serum IL-6 level.
abstract: OBJECTIVE
  To study the extent of periodontal disease and the IL-6(-174) genotype as determinants of serum and mouthwash IL-6 concentration in subjects with moderate to severe periodontal disease.
  
  
  METHODS
  Fifty-two generally healthy subjects volunteered to participate. Probing pocket depth (PD) and periodontal attachment level (AL) were clinically examined and alveolar bone level (BL) was measured on orthopantomographs. IL-6 concentrations in mouthwash, collected by rinsing with 3 ml saline for 30 s and in serum, obtained by venipuncture, were measured using ELISA. IL-6(-174) polymorphism was studied using a polymerase chain reaction.
  
  
  RESULTS
  Eleven subjects carried the GG genotype, and 41 subjects, carried the CG/CC genotype. The mean (+/- SD) concentration of IL-6 in serum was 1.6 (+/- 1.5) pg/ml and, 2.8 (+/- 5.04) pg/ml in mouthwash. The serum concentration of IL-6 was higher in subjects with the GG genotype than with the CG/CC genotype. In regression analyses the percentages of sites with PD> or =6 mm, AL> or =6 mm and BL> or =8 mm, the IL-6(-174) genotype, body mass index and gender associated significantly with serum IL-6 concentration.
  
  
  CONCLUSIONS
  The extent of moderate to severe periodontal disease and the IL-6(-174) genotype contribute significantly to serum IL-6 concentration.
authors.affiliation: The Dental Specialist Clinic of Oulu Municipal Health Center, Oulu, Finland.
authors.forename: Taina
authors.initials: T
authors.lastname: Raunio
authors.forename: Marianne
authors.initials: M
authors.lastname: Nixdorf
authors.forename: Matti
authors.initials: M
authors.lastname: Knuuttila
authors.forename: Riitta
authors.initials: R
authors.lastname: Karttunen
authors.forename: Olli
authors.initials: O
authors.lastname: Vainio
authors.forename: Tellervo
authors.initials: T
authors.lastname: Tervonen
mesh_terms: D000328:Adult; D004812:Epidemiologic Methods; D005260:Female; D005838:Genotype; D006801:Humans; D015850:Interleukin-6 / Q000097:blood* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009067:Mouthwashes / Q000032:analysis / Q000737:chemistry; D010510:Periodontal Diseases / Q000097:blood* / Q000000981:diagnostic imaging / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics*; D011859:Radiography; D012907:Smoking / Q000097:blood
chemical_list: D015850:Interleukin-6; D009067:Mouthwashes