[Detecting VA, VD3, VE and beta-carotene with HPLC in human serums].
OBJECTIVE
To develop a method of detecting retinol (vitamin A), vitamin D3, alpha-tocopherol (vitamin E) and beta-carotene in human serums with HPLC.


METHODS
Proteins were precipitated with anhydrous ethanol. Fat-solutable vitamins in human serums were extracted with aether/petroleum ether mixture and determined with HPLC.


RESULTS
The linear ranges for the retinol, VD3, alpha-tocopherol and beta-carotene were 0.012 microg/mL-500 microg/mL, 0.030 microg/mL-500 microg/mL, 0.12 microg/mL-500 microg/mL, and 0.015 microg/mL-500 microg/mL, respectively. The detection limits for the retinol, VD3, alpha-tocopherol and beta-carotene were 0.012 microg/mL, 0.030 microg/mL, 0.12 microg/mL and 0.015 microg/mL, respectively. The relative standard derivations (RSD) for the retinol, VD3, alpha-tocopherol and beta-carotene were 0.75%, 0.54%, 2.06% and 2.74%, respectively. The proposed method recovered 92%-116%, 98%-112%, 84.8%-106%, and 90%-105% retinol, VD3, alpha-tocopherol and beta-carotene in human serums respectively.


CONCLUSIONS
The method is simple, quick and applicable to all of the four fat-solutable vitamins.
Zou
Xiao-li
XL
Department of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Zeng
Hong-yan
HY
Li
Yuan-qian
YQ
Li
Xiao-xia
XX
Zhang
Si-wei
SW
Zheng
Bo
B
Huang
Cheng-yu
CY
D002762:Cholecalciferol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D014801:Vitamin A / Q000032:analysis*; D024502:alpha-Tocopherol / Q000032:analysis*; D019207:beta Carotene / Q000032:analysis*
D019207:beta Carotene; D014801:Vitamin A; D002762:Cholecalciferol; D024502:alpha-Tocopherol