Fiber-optic immuno-biosensor for rapid and accurate detection of nerve growth factor in human blood.
An accurate and rapid assay of cardiac nerve growth factor (NGF) levels in blood can provide physicians with critical information regarding myocardial injury and neural remodeling in cardiac tissues to identify patients at risk of impending heart attack, thereby enabling them to receive appropriate lifesaving treatment more quickly. Currently used assay methods, such as enzyme-linked immunosorbent assay (ELISA), are usually time-consuming (hours to days), expensive and technically complicated. In this paper, we described the development and clinical study of a rapid and sensitive method for detection and quantification of NGF in human blood plasma. This method utilizes a fiber-optic, immuno-biosensing system which performs a fluorophore-mediated sandwich immunoassay on the surface of an optical fiber. Physiological concentrations of NGF could be quantified in both buffer and human blood plasma samples within 5 minutes. The NGF concentrations determined by the fiberoptic sensor were comparable to those by the gold standard, ELISA. Preliminary study of NGF assay in cardiac patient plasma samples showed a great potential of the fiber-optic sensor as a rapid diagnostic and prognostic tool in clinical applications.
Tang
Liang
L
Division of Cardiology, Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, USA. liang.tang@cshs.org
Cha
Yong-Mei
YM
Li
Hongmei
H
Chen
Peng-Sheng
PS
Lin
Shien-Fong
SF
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D020932:Nerve Growth Factor / Q000097:blood*; D055100:Optical Fibers; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D020932:Nerve Growth Factor