Detection of Ehrlichia muris DNA from sika deer (Cervus nippon yesoensis) in Hokkaido, Japan.
Ehrlichia muris DNA was detected in the blood of sika deer (Cervus nippon yesoensis) by species-specific PCR based on the citrate synthase gene, which was shown to be more sensitive than species-specific PCR based on the 16S rRNA gene. Among 102 deer examined, one deer was positive. Deer may be a possible mammalian reservoir of E. muris.
Tamamoto
Chie
C
Department of Veterinary Clinical Science, Obihiro University of Veterinary Medicine & Agriculture, Obihiro, Japan.
Seino
Nobutaka
N
Suzuki
Masatsugu
M
Kaji
Koichi
K
Takahashi
Hiroshi
H
Inokuma
Hisashi
H
D000818:Animals; D002950:Citrate (si)-Synthase / Q000235:genetics*; D004269:DNA, Bacterial / Q000097:blood*; D003670:Deer / Q000382:microbiology*; D004197:Disease Reservoirs / Q000662:veterinary; D004536:Ehrlichia / Q000201:enzymology / Q000302:isolation & purification*; D016873:Ehrlichiosis / Q000453:epidemiology / Q000662:veterinary*; D007564:Japan / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D013045:Species Specificity
D004269:DNA, Bacterial; D002950:Citrate (si)-Synthase