title: Use of sera from humans and dolphins with lacaziosis and sera from experimentally infected mice for Western Blot analyses of Lacazia loboi antigens.
abstract: Antibodies in the sera of patients with lacaziosis recognized an approximately 193-kDa antigen and other Lacazia loboi antigens. Paracoccidioides brasiliensis gp43 antigen was detected by all evaluated sera, but they failed to detect a protein with the same molecular mass in L. loboi extracts. This study is the first to examine the humoral response to L. loboi antigens by using multiple host sera.
authors.affiliation: Biomedical Laboratory Diagnostics Program, Michigan State University, 322 North Kedzie Hall, East Lansing, MI 48824-1031, USA. mendoza9@msu.edu
authors.forename: Leonel
authors.initials: L
authors.lastname: Mendoza
authors.forename: Andréa F F
authors.initials: AF
authors.lastname: Belone
authors.forename: Raquel
authors.initials: R
authors.lastname: Vilela
authors.forename: Manuela
authors.initials: M
authors.lastname: Rehtanz
authors.forename: Gregory D
authors.initials: GD
authors.lastname: Bossart
authors.forename: John S
authors.initials: JS
authors.lastname: Reif
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Fair
authors.forename: Wendy N
authors.initials: WN
authors.lastname: Durden
authors.forename: Judy
authors.initials: J
authors.lastname: St Leger
authors.forename: Luiz R
authors.initials: LR
authors.lastname: Travassos
authors.forename: Patricia S
authors.initials: PS
authors.lastname: Rosa
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology / Q000302:isolation & purification; D000946:Antigens, Fungal / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification; D015153:Blotting, Western / Q000379:methods*; D050681:Bottle-Nosed Dolphin; D005656:Fungal Proteins / Q000097:blood / Q000276:immunology; D006023:Glycoproteins / Q000097:blood / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D009181:Mycoses / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D020036:Onygenales / Q000276:immunology* / Q000302:isolation & purification; D010228:Paracoccidioides / Q000276:immunology / Q000302:isolation & purification; D010229:Paracoccidioidomycosis / Q000097:blood / Q000175:diagnosis / Q000382:microbiology
chemical_list: C103180:43 kDa protein, Paracoccidioides; D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D005656:Fungal Proteins; D006023:Glycoproteins; D007074:Immunoglobulin G