Transcriptional regulation of genes on the non-syntenic blocks of Aspergillus oryzae and its functional relationship to solid-state cultivation.
Transcriptome analysis revealed close relationship between solid-state cultivation and the transcriptional regulation of the genes on the non-syntenic blocks (NSBs), which were characterized by the comparison of Aspergillus oryzae genome with those of Aspergillus fumigatus and Aspergillus nidulans. Average expression ratio of the genes on NSBs in solid-state cultivation was significantly higher than that on the syntenic blocks (SBs). Of the induced genes, the genes relating to metabolism, which are highly enriched on NSBs, most contributed to the NSB-specific induction. The analysis using the SB- and NSB-genes that had sequence similarity between the two blocks significantly decreased the difference of average expression ratios between the two blocks. In spite of remarkably high averaged expression ratio of the NSB genes encoding extracellular enzymes, no induction of PKS and NRPS genes on NSBs were observed in solid-state cultivations. These results strongly suggest that the genes on NSBs play an important role on solid-state fermentation.
Tamano
Koichi
K
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan.
Sano
Motoaki
M
Yamane
Noriko
N
Terabayashi
Yasunobu
Y
Toda
Tomomi
T
Sunagawa
Misao
M
Koike
Hideaki
H
Hatamoto
Osamu
O
Umitsuki
Genryou
G
Takahashi
Tadashi
T
Koyama
Yasuji
Y
Asai
Ryoichi
R
Abe
Keietsu
K
Machida
Masayuki
M
D001236:Aspergillus oryzae / Q000235:genetics* / Q000254:growth & development; D003470:Culture Media; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007218:Industrial Microbiology; D020411:Oligonucleotide Array Sequence Analysis
D003470:Culture Media