Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) Protein (Na-ASP1).
Human hookworm infection is one of the most significant parasitic infections, and a leading global cause of anemia and malnutrition of adults and children in rural areas of the tropics and subtropics. Necator americanus secretory protein (Na-ASP1), which is a potential vaccine candidate against hookworm infections, has been expressed in Pichia pastoris. Na-ASP1 protein was expressed extracellulary by employing the leader sequence of the alpha-mating factor of Saccharomyces cerevisiae. Most of the protein produced by single copy clones was secreted outside the cell. The Na-ASP1 steady state mRNA levels of the clones were correlated to their Na-ASP1 gene copy number. However, increasing gene copy number of Na-ASP1 protein in P. pastoris saturated secretory capacity and therefore, decreased the amount of secreted protein in clones harboring multiple copies of Na-ASP1 gene.
Inan
Mehmet
M
Department of Chemical Engineering, University of Nebraska, Lincoln, NE. USA.
Fanders
Sarah A
SA
Zhang
Wenhui
W
Hotez
Peter J
PJ
Zhan
Bin
B
Meagher
Michael M
MM
D000723:Ancylostomatoidea / Q000378:metabolism*; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002999:Clone Cells; D003062:Codon; D018628:Gene Dosage; D015870:Gene Expression; D005822:Genetic Vectors; D015801:Helminth Proteins / Q000235:genetics*; D010843:Pichia / Q000235:genetics; D014170:Transformation, Genetic
D003062:Codon; D015801:Helminth Proteins