Development of mature cartilage constructs using novel three-dimensional porous scaffolds for enhanced repair of osteochondral defects.
In this study, we successfully developed two types of volume-reduced three-dimensional scaffolds, including cushion- and cylinder-shape scaffolds, fabricated from chitosan-based hyaluronic acid hybrid polymer fibers. Using these scaffolds combined with a bioreactor system, we regenerated histologically and mechanically mature cartilage constructs. The final goal of this study was to clarify the ability of this engineered cartilage construct to induce cartilage repair in osteochondral defects. The mature cartilage constructs regenerated with two types of scaffolds were implanted into 5-mm diameter osteochondral defects in the patellar groove of rabbits. At 12 weeks after implantation, the reparative tissues consisted of hyaline-like cartilage with evidence of stable fusion to adjacent native cartilage and normal reconstitution of subchondral bone. The histological score of these tissues significantly outranked the value of untreated tissue. Biomechanically, compression modulus of reparative tissue at 12 weeks postoperatively was comparative to that of normal articular cartilage. Our results indicate that the implantation of constructs with mature cartilage have potential as a better approach for joint resurfacing.
Kasahara
Yasuhiko
Y
Department of Orthopaedic Surgery, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
Iwasaki
Norimasa
N
Yamane
Shintaro
S
Igarashi
Tatsuya
T
Majima
Tokifumi
T
Nonaka
Sachiko
S
Harada
Kazuo
K
Nishimura
Shin-Ichiro
S
Minami
Akio
A
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001696:Biomechanical Phenomena; D018786:Bone Substitutes; D002356:Cartilage / Q000473:pathology*; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D019902:Chondrocytes / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry / Q000379:methods; D023421:Models, Animal; D011817:Rabbits; D012038:Regeneration; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D014945:Wound Healing
D001672:Biocompatible Materials; D018786:Bone Substitutes; D004247:DNA