title: Direct detection of soil-bound prions.
abstract: Scrapie and chronic wasting disease are contagious prion diseases affecting sheep and cervids, respectively. Studies have indicated that horizontal transmission is important in sustaining these epidemics, and that environmental contamination plays an important role in this. In the perspective of detecting prions in soil samples from the field by more direct methods than animal-based bioassays, we have developed a novel immuno-based approach that visualises in situ the major component (PrP(Sc)) of prions sorbed onto agricultural soil particles. Importantly, the protocol needs no extraction of the protein from soil. Using a cell-based assay of infectivity, we also report that samples of agricultural soil, or quartz sand, acquire prion infectivity after exposure to whole brain homogenates from prion-infected mice. Our data provide further support to the notion that prion-exposed soils retain infectivity, as recently determined in Syrian hamsters intracerebrally or orally challenged with contaminated soils. The cell approach of the potential infectivity of contaminated soil is faster and cheaper than classical animal-based bioassays. Although it suffers from limitations, e.g. it can currently test only a few mouse prion strains, the cell model can nevertheless be applied in its present form to understand how soil composition influences infectivity, and to test prion-inactivating procedures.
authors.affiliation: Dipartimento di Chimica Biologica, Università di Padova, Padova, Italy.
authors.forename: Sacha
authors.initials: S
authors.lastname: Genovesi
authors.forename: Liviana
authors.initials: L
authors.lastname: Leita
authors.forename: Paolo
authors.initials: P
authors.lastname: Sequi
authors.forename: Igino
authors.initials: I
authors.lastname: Andrighetto
authors.forename: M Catia
authors.initials: MC
authors.lastname: Sorgato
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bertoli
mesh_terms: D000818:Animals; D001671:Biochemistry / Q000379:methods; D001681:Biological Assay; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D006224:Cricetinae; D004195:Disease Models, Animal; D008647:Mesocricetus; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018620:PrPSc Proteins / Q000302:isolation & purification / Q000378:metabolism*; D017096:Prion Diseases / Q000635:transmission*; D011328:Prions / Q000302:isolation & purification / Q000378:metabolism*; D012987:Soil
chemical_list: D018620:PrPSc Proteins; D011328:Prions; D012987:Soil