title: [The effects of nm23-H1 gene cell transfection on abilities of invasion, adhesion and chemotherapeutic sensitivity of bladder cancer T-24 cell line].
abstract: OBJECTIVE
  To investigate the transfection and expression of nm23-H1 gene in human bladder cancer cell line T-24, and to find out whether nm23-H1 could affect the metastases ability and the chemotherapeutic sensitivity of T-24 cell line.
  
  
  METHODS
  By electroporation way, the pCMV-Bam-neo-nm23-H1 plasmid was transferred into bladder cancer line T-24, and the nm23-H1 vector-producer cell line T-24/nm23-H1 was isolated with G418 selection. The expression of nm23-H1 gene q,as detected by immunohistochemistry. The T-24 invasion, adherence and migration abilities between transfected and non-transfected cell lines were detected by transwell-room and wash techniques. The difference or change to the sensitivity of chemotherapeutic drug before and after cell transfected was evaluated by MTT.
  
  
  RESULTS
  The stable expression of nm23-H1 was obtained by pCMV-Bam-Neo system with G418. The expression of nucleoside diphosphate kinase-A (NDPKA) was observed for significant difference between transfected and non-transfected T-24 cell lines; The invasive, adherence and migration abilities of transfected T-24 cells were decreased more than those of non-transfected T-24 (P < 0. 05); The chemo-sensitivity of transfected T-24 line cells to cis-diamminedichloroplatinum (CDDP) increased significantly.
  
  
  CONCLUSIONS
  nm23-H1 gene can inhibit the metastasis of T-24 cell line and increase the chemo-sensitivity of T-24 cell line to CDDP significantly.
authors.affiliation: Department of Urology, the Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Cui
authors.forename: Tie-long
authors.initials: TL
authors.lastname: Tang
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Li
mesh_terms: D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D005822:Genetic Vectors; D006801:Humans; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics*; D009362:Neoplasm Metastasis; D010957:Plasmids; D014162:Transfection; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy / Q000235:genetics*
chemical_list: D054778:NM23 Nucleoside Diphosphate Kinases; C517938:NME1 protein, human; D002945:Cisplatin