[Expressions of cytoprotective proteins for human renal allografts].
OBJECTIVE
To investigate the expression status and clinical significance of cytoprotective proteins to human renal allografts.


METHODS
Expressions of cytoprotective proteins: antiapoptotic protein (A20), hemeoxygenase-1 (HO-1), and B-cell lymphoma/leukemia-X(L) (Bcl-X(L)) were analyzed by immunohistochemistry and compared in 30 renal allografts, including 10 grafts undergoing acute rejection (AR), 10 grafts undergoing chronic rejection (CR), and 10 nonrejecting (NR) grafts.


RESULTS
Expressions of A20, HO-1 and Bcl-X(L) localized mainly in endothelial, smooth muscle, and infiltrating mononuclear cells. A20 expressed in grafts undergoing AR (5.04 +/- 0.71) and CR (3.20 +/- 0.64), not in NR grafts, and its expression in CR was weaker than that in AR ( P < 0.01). HO-1 expressed in AR (7.91 +/- 2.24), not in CR and NR. Bcl-X(L) was detected in all grafts (AR: 10.62 +/- 3.17; CR: 8.50 +/- 2.45; NR: 11.03 +/- 2.77), but had a decreased expression levels in CR.


CONCLUSIONS
A20 and HO-1 may play protective role for AR, and A20 may be the essential protein having protective function for CR.
Teng
Dong-hai
DH
Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
Lu
Yi-ping
YP
Cao
Gui-hau
GH
Zhang
Hong-ying
HY
Li
Xiang
X
Wang
Li
L
Wang
Jia
J
Li
You-ping
YP
D004268:DNA-Binding Proteins; D006084:Graft Rejection / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D016030:Kidney Transplantation; D009687:Nuclear Proteins / Q000378:metabolism*; D014184:Transplantation, Homologous; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3; D051020:bcl-X Protein / Q000378:metabolism*
C494811:BCL2L1 protein, human; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D051020:bcl-X Protein; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1; C076529:TNFAIP3 protein, human; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3