Eye drop NGF administration promotes the recovery of chemically injured cholinergic neurons of adult mouse forebrain.
We have recently shown that conjunctivally applied nerve growth factor (NGF) in rats can reach the retina, the optic nerve and the CNS. In the present study, we investigated whether NGF application as collyrium can promote the recovery of chemically injured basal forebrain cholinergic neurons. NGF was administered on the eye of adult male mice previously treated i.c.v. with ibotenic acid to impair cholinergic pathways. Mice were tested in the passive avoidance test, and after 2 weeks of NGF administration were killed and the brains used for structural, biochemical and molecular analyses. The results showed that application of NGF on the eye surface protected choline acetyl transferase levels. These findings strengthen the hypothesis that application of NGF on the eye can represent an alternative delivery route to promote the recovery of brain cells during degeneration, including neurons involved in learning and memory activities.
Di Fausto
Veronica
V
Institute of Neurobiology and Molecular Medicine, Department of Neurobiology, National Research Council (CNR) Rome, Italy.
Fiore
Marco
M
Tirassa
Paola
P
Lambiase
Alessandro
A
Aloe
Luigi
L
D000109:Acetylcholine / Q000096:biosynthesis; D000818:Animals; D020532:Basal Nucleus of Meynert / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D002540:Cerebral Cortex / Q000201:enzymology; D002795:Choline O-Acetyltransferase / Q000187:drug effects / Q000378:metabolism; D002799:Cholinergic Fibers / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D003714:Denervation; D004195:Disease Models, Animal; D006624:Hippocampus / Q000201:enzymology; D007051:Ibotenic Acid; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D009410:Nerve Degeneration / Q000139:chemically induced / Q000188:drug therapy* / Q000517:prevention & control; D020932:Nerve Growth Factor / Q000008:administration & dosage*; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009883:Ophthalmic Solutions; D020127:Recovery of Function / Q000187:drug effects* / Q000502:physiology; D016896:Treatment Outcome
D009883:Ophthalmic Solutions; D007051:Ibotenic Acid; D020932:Nerve Growth Factor; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine