title: Endoscopic tri-modal imaging for detection of early neoplasia in Barrett's oesophagus: a multi-centre feasibility study using high-resolution endoscopy, autofluorescence imaging and narrow band imaging incorporated in one endoscopy system.
abstract: OBJECTIVE
  To investigate the diagnostic potential of endoscopic tri-modal imaging and the relative contribution of each imaging modality (i.e. high-resolution endoscopy (HRE), autofluorescence imaging (AFI) and narrow-band imaging (NBI)) for the detection of early neoplasia in Barrett's oesophagus.
  
  
  METHODS
  Prospective multi-centre study.
  
  
  METHODS
  Tertiary referral centres.
  
  
  METHODS
  84 Patients with Barrett's oesophagus.
  
  
  METHODS
  The Barrett's oesophagus was inspected with HRE followed by AFI. All lesions detected with HRE and/or AFI were subsequently inspected in detail by NBI for the presence of abnormal mucosal and/or microvascular patterns. Biopsies were obtained from all suspicious lesions for blinded histopathological assessment followed by random biopsies.
  
  
  METHODS
  (1) Number of patients with early neoplasia diagnosed by HRE and AFI; (2) number of lesions with early neoplasia detected with HRE and AFI; and (3) reduction of false positive AFI findings after NBI.
  
  
  RESULTS
  Per patient analysis: AFI identified all 16 patients with early neoplasia identified with HRE and detected an additional 11 patients with early neoplasia that were not identified with HRE. In three patients no abnormalities were seen but random biopsies revealed HGIN. After HRE inspection, AFI detected an additional 102 lesions; 19 contained HGIN/EC (false positive rate of AFI after HRE: 81%). Detailed inspection with NBI reduced this false positive rate to 26%.
  
  
  CONCLUSIONS
  In this international multi-centre study, the addition of AFI to HRE increased the detection of both the number of patients and the number of lesions with early neoplasia in patients with Barrett's oesophagus. The false positive rate of AFI was reduced after detailed inspection with NBI.
authors.affiliation: Department of Gastroenterology and Hepatology, Academic Medical Centre, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands.
authors.forename: W L
authors.initials: WL
authors.lastname: Curvers
authors.forename: R
authors.initials: R
authors.lastname: Singh
authors.forename: L-M Wong-Kee
authors.initials: LM
authors.lastname: Song
authors.forename: H C
authors.initials: HC
authors.lastname: Wolfsen
authors.forename: K
authors.initials: K
authors.lastname: Ragunath
authors.forename: K
authors.initials: K
authors.lastname: Wang
authors.forename: M B
authors.initials: MB
authors.lastname: Wallace
authors.forename: P
authors.initials: P
authors.lastname: Fockens
authors.forename: J J G H M
authors.initials: JJ
authors.lastname: Bergman
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001471:Barrett Esophagus / Q000473:pathology*; D016145:Endoscopy, Digestive System / Q000379:methods*; D004938:Esophageal Neoplasms / Q000473:pathology*; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods; D008875:Middle Aged; D011230:Precancerous Conditions / Q000473:pathology*