title: Virus-like particle and viral vector production using the baculovirus expression vector system/insect cell system: adeno-associated virus-based products.
abstract: The ability to make a large variety of virus-like particles (VLPs) has been successfully achieved in the baculovirus expression vector system (BEVS)/insect cell system. The production and scale-up of these particles, which are mostly sought as candidate vaccines, are currently being addressed. Furthermore, these VLPs are being investigated as delivery agents for use as therapeutics. Recently, adeno-associated viral (AAV) vectors, which can be potentially used for human gene therapy, have been produced in insect cells using three baculovirus vectors to supply the required genes. The use of host insect cells allows mass production of VLPs in a proven scaleable system. This chapter focuses on the methodology, based on the work done in our lab, for the production of AAV-like particles and vectors in a BEVS/insect cell system.
authors.affiliation: Animal Cell Technology Group, Biotechnology Research Institute, National Research Council Canada, Montreal, Canada.
authors.forename: Marc G
authors.initials: MG
authors.lastname: Aucoin
authors.forename: Danielle
authors.initials: D
authors.lastname: Jacob
authors.forename: Parminder Singh
authors.initials: PS
authors.lastname: Chahal
authors.forename: Jamal
authors.initials: J
authors.lastname: Meghrous
authors.forename: Alice
authors.initials: A
authors.lastname: Bernier
authors.forename: Amine A
authors.initials: AA
authors.lastname: Kamen
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D015153:Blotting, Western; D002460:Cell Line; D000229:Dependovirus / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D007313:Insecta / Q000166:cytology / Q000821:virology*