Encapsulation of protein drugs in biodegradable microparticles by co-axial electrospray.
A co-axial electrospray process was developed to encapsulate protein-based drugs in biodegradable polymeric microparticles eliminating the key problem faced by other conventional methods of protein encapsulation--the primary emulsion being a major cause for protein denaturation and aggregation. Bovine serum albumin (BSA) and lysozyme were chosen as model protein drugs for this study. Scanning electron microscopy observation of the morphology of particles showed spherical microparticles of several microns could be achieved. In vitro release profiles measured using Micro-BCA assay indicated sustained release of proteins for more than 30 days. The results of circular dichroism suggested that the secondary structure of released BSA can be retained. The bioactivity of released lysozyme was found to be more than 90% which is higher than the values reported from most literatures. Therefore, co-axial electrospray could be a very promising approach to encapsulate biomacromolecules such as proteins, enzymes, DNA plasmids or living cells inside microparticles for controlled release drug delivery applications.
Xie
Jingwei
J
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, 117576 Singapore.
Ng
Wei Jun
WJ
Lee
Lai Yeng
LY
Wang
Chi-Hwa
CH
D000818:Animals; D001672:Biocompatible Materials; D002417:Cattle; D002942:Circular Dichroism; D004339:Drug Compounding / Q000379:methods*; D016503:Drug Delivery Systems; D019344:Lactic Acid; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008863:Microspheres; D009113:Muramidase / Q000008:administration & dosage; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D017433:Protein Structure, Secondary; D011506:Proteins / Q000008:administration & dosage*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage
D001672:Biocompatible Materials; D011108:Polymers; D011506:Proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D009113:Muramidase