title: Use of polymerase chain reaction for the identification of Leptospira sp. in urine of carriers.
abstract: The objectives of the present work were to detect Leptospira seropositive animals. The ELISA results report only IgG antibodies, which could be attributable to chronic infections or else, that they are healthy carriers. All polymerase chain reaction positive animals should be considered potential sources of infection.
authors.affiliation: CENID Microbiología-INIFAP, México.
authors.forename: Victor Manuel Banda
authors.initials: VM
authors.lastname: Ruiz
authors.forename: Laura Elena Orozco
authors.initials: LE
authors.lastname: Vega
authors.forename: Rosa Maria Urrutia
authors.initials: RM
authors.lastname: Velázquez
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000032:analysis; D002353:Carrier State / Q000382:microbiology*; D002417:Cattle / Q000382:microbiology*; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007919:Leptospira / Q000235:genetics* / Q000302:isolation & purification*; D007921:Leptospira interrogans / Q000276:immunology; D007922:Leptospirosis / Q000175:diagnosis / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D008800:Mexico; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D014556:Urine / Q000382:microbiology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D007074:Immunoglobulin G