Revisiting TRPC1 and TRPC6 mechanosensitivity.
This article addresses whether TRPC1 or TRPC6 is an essential component of a mammalian stretch-activated mechano-sensitive Ca(2+) permeable cation channel (MscCa). We have transiently expressed TRPC1 and TRPC6 in African green monkey kidney (COS) or Chinese hamster ovary (CHO) cells and monitored the activity of the stretch-activated channels using a fast pressure clamp system. Although both TRPC1 and TRPC6 are highly expressed at the protein level, the amplitude of the mechano-sensitive current is not significantly altered by overexpression of these subunits. In conclusion, although several TRPC channel members, including TRPC1 and TRPC6, have been recently proposed to form MscCa in vertebrate cells, the functional expression of these TRPC subunits in heterologous systems remains problematic.
Gottlieb
Philip
P
Single Molecule Biophysics, SUNY, 301 Cary Hall, Buffalo, NY 14214, USA.
Folgering
Joost
J
Maroto
Rosario
R
Raso
Albert
A
Wood
Thomas G
TG
Kurosky
Alex
A
Bowman
Charles
C
Bichet
Delphine
D
Patel
Amanda
A
Sachs
Frederick
F
Martinac
Boris
B
Hamill
Owen P
OP
Honoré
Eric
E
D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D008465:Mechanoreceptors / Q000502:physiology*; D018613:Microscopy, Confocal; D018408:Patch-Clamp Techniques; D010957:Plasmids; D050052:TRPC Cation Channels / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D000074287:TRPC6 Cation Channel; D014162:Transfection
D050052:TRPC Cation Channels; D000074287:TRPC6 Cation Channel; C117032:TRPC6 protein, human; C096351:transient receptor potential cation channel, subfamily C, member 1