[Prokaryotic expression of HLA-B * 2705 heavy chain and identification for its activity].
In order to investigate the expression of heavy chain of HLA-B * 2705 in prokaryotic system and identify its activity, the extra-membrane gene fragment of HLA-B * 2705 was amplified from full-length HLA-B*2705 cDNA by PCR and cloned into pGEM-T vector. After identification by sequencing, the prokaryotic expressing vector pET32a (+)-B * 2705 was constructed. The antigenic activity of expressed protein was identified by Western blot and antibody blocking reaction. The results indicated that the fused HLA-B * 2705 protein expression with high efficiency was obtained. The expressed product was more than 50% of the total bacteria protein. The antigenic activity of expressed protein was confirmed by Western blot and antibody blocking reaction. It is concluded that HLA-B * 2705 fusion protein are obtained as basis for the further studies.
Li
Yan-Bo
YB
Department of Immunology, Affiliated Hospital of Academy of Military Medical Sciences, Laboratory of Immunoassy, National Center of Biomedical Analysis, Beijing 100039, China.
Sun
Yu-Ying
YY
Guo
Si-Qi
SQ
Sun
Ye-Ping
YP
Zhang
Xiu-Yun
XY
Kong
Fan-Hua
FH
Xi
Yong-Zhi
YZ
D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics*; D015796:HLA-B27 Antigen / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
C464936:HLA-B*27:05 antigen; D015796:HLA-B27 Antigen; D007143:Immunoglobulin Heavy Chains