[Isolation and identification of the mixture of L-hydroxyproline oligo-peptides by reversed-phase high performance liquid chromatography and electrospray ionization tandem mass spectrometry].
A reversed-phase high performance liquid chromatographic-electrospray ionization tandem mass spectrometric method (RP-HPLC/MS2) was developed for the analysis and identification of a reaction mixture of L-hydroxyproline oligo-peptides. The mixture was separated on a YWG C8 column (10 microm, 250 mm x 10 mm) using acetonitrile-water containing 0.06% trifluoroacetic acid (2:98, v/v) as the mobile phase. The separation conditions were optimized. The mixture was analysed with electrospray ionization mass spectrometry (ESI-MS) and ESI-MS/MS in positive mode. MS and MS2 spectra of individual chromatographic peak were obtained. It indicated that there were hydroxyproline dipeptide, hydroxyproline tripeptide and hydroxyproline cyclo-dipeptide in the mixtures, besides hydroxyproline. The described method can be effectively applied to perform identification of hydroxyproline oligo-peptide mixtures.
Sun
Yanting
Y
School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450052, China. sun-yanting@163.com
Lu
Kui
K
Ma
Li
L
Cao
Shuxia
S
Zhao
Yufen
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006909:Hydroxyproline / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry* / Q000302:isolation & purification*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D009842:Oligopeptides; D006909:Hydroxyproline