Classical swine fever virus inhibits nitric oxide production in infected macrophages.
Classical swine fever virus (CSFV)-macrophage interactions during infection were analysed by examining macrophage transcriptional responses via microarray. Eleven genes had increased mRNA levels (>2.5-fold, P<0.05) in infected cell cultures, including arginase-1, an inhibitor of nitric oxide production, phosphoinositide 3-kinase, chemokine receptor 4 and interleukin-1beta. Lower levels of nitric oxide and increased arginase activity were found in CSFV-infected macrophages. These changes in gene expression in macrophages suggest viral modulation of host expression to suppress nitric oxide production.
Zaffuto
K M
KM
Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
Piccone
M E
ME
Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
Burrage
T G
TG
Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
Balinsky
C A
CA
Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
Risatti
G R
GR
Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
Borca
M V
MV
Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
Holinka
L G
LG
Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
Rock
D L
DL
Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
Afonso
C L
CL
Plum Island Animal Disease Center, USDA-ARS, PO Box 848, Greenport, NY 11944, USA.
D000818:Animals; D001119:Arginase / Q000032:analysis / Q000235:genetics; D002478:Cells, Cultured; D006692:Classical Swine Fever Virus / Q000276:immunology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008264:Macrophages / Q000276:immunology* / Q000821:virology*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D013552:Swine
D012333:RNA, Messenger; D009569:Nitric Oxide; D001119:Arginase