[Atorvastatin inhibits scavenger receptor A and monocyte chemoattractant protein-1 expressions in foam cell].
OBJECTIVE
To investigate the effects of atorvastatin on expressions of scavenger receptor A and secretion of monocyte chemoattractant protein-1 (MCP-1) in foam cells.


METHODS
THP-1 cells were induced to differentiate into macrophages by PMA and treated with 0.1% BSA (control), ox-LDL (100 mg/L) or ox-LDL plus atorvastatin (5, 10, 20 micromol/L) for 24 hours. MCP-1 concentration in cell substratum was measured by ELISA. Scavenger receptor A expression was observed under fluorescent microscope after incubated with DiI-Ac-LDL. The relationship between concentration of MCP-1 and the activity of scavenger receptor A was also analyzed.


RESULTS
Compared to the control cells, MCP-1 concentration in ox-LDL treated cells was significantly increased after 6 hours, peaked at 12 hours and was still significantly increased after 24 hours (all P < 0.05 vs. baseline). The activity of scavenger receptor A was also significantly increased in ox-LDL treated cells (P < 0.01 vs. control). The activity of scavenger receptor A proteins correlated positively to the concentration of MCP-1 in ox-LDL treated cells (r = 0.683, P < 0.01). Atorvastatin significantly attenuated these changes in a dose-dependent manner.


CONCLUSIONS
Scavenger receptor A and MCP-1 expressions were significantly increased in the course of monocyte lines THP-1 differentiating into macrophages and foam cells. The anti-atherosclerosis effect of atorvastatin might be partly achieved by inhibiting the secretion of MCP-1 and expression of scavenger receptor A in foam cells.
Zhu
Gui-yue
GY
Department of Cardiology, Shandong Provincial Hospital, Shandong University, Jinan 250021, China. zgymd@163.com
Zhu
Xing-lei
XL
Li
Ren-tiao
RT
Liu
Tong-bao
TB
Shang
De-ya
DY
Zhang
Yun
Y
D000069059:Atorvastatin; D002454:Cell Differentiation; D002460:Cell Line; D018932:Chemokine CCL2 / Q000378:metabolism*; D005487:Foam Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006538:Heptanoic Acids / Q000494:pharmacology*; D006801:Humans; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011758:Pyrroles / Q000494:pharmacology*; D051117:Scavenger Receptors, Class A / Q000378:metabolism*
C111835:CCL2 protein, human; D018932:Chemokine CCL2; D006538:Heptanoic Acids; D011758:Pyrroles; D051117:Scavenger Receptors, Class A; D000069059:Atorvastatin