In vitro antimicrobial activity of plants used in Cambodian traditional medicine.
The purpose of the present study was to screen 27 plant species used in the traditional medicine of Cambodia for in vitro antibacterial and antifungal activities. Thirty-three methanolic extracts were tested against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Mycobacterium smegmatis and Candida albicans. Screened by disk diffusion assay, the extracts showed antimicrobial activity especially on Gram-positive bacteria. None of the crude methanolic extracts showed activity against P. aeruginosa. Twenty-five selected extracts were evaluated using a micro-dilution test. Harrisonia perforata (roots) and Hymenodictyon excelsum (bark) exhibited a bactericidal effect against S. aureus at a concentration of 500 microg/ml. Azadirachta indica (bark), Harrisonia perforata (roots and stem) and Shorea obtusa (roots) exhibited a bactericidal effect against M. smegmatis at 250 microg/ml.
Chea
Aun
A
Laboratory of Pharmacognosy, EA864, Faculty of Pharmacy, Marseille, France.
Jonville
Marie-Caroline
MC
Bun
Sok-Siya
SS
Laget
Michèle
M
Elias
Riad
R
Duménil
Gérard
G
Balansard
Guy
G
D000890:Anti-Infective Agents / Q000494:pharmacology*; D002160:Cambodia; D003470:Culture Media; D008519:Medicine, Traditional; D008826:Microbial Sensitivity Tests; D010946:Plants, Medicinal
D000890:Anti-Infective Agents; D003470:Culture Media