Chemiluminescent probe for the in vitro detection of protease activity.
A strategy involving the use of a self-immolative linker has been investigated for the chemiluminescent sensing of proteases. The reactive linker enabled the release of a 1,2-dioxetane light precursor. As a proof of principle, caspase-3, a key peptidase involved in apoptosis has been targeted. An in vitro assay has been carried out and proved the decomposition of the linker and the selectivity for caspase-3.
Richard
Jean-Alexandre
JA
Equipe de Chimie Bio-Organique, Université de Rouen, INSA de Rouen, CNRS UMR 6014, COBRA, IRCOF, 1 rue Lucien Tesnière, Mont-Saint-Aignan Cedex, France
Jean
Ludovic
L
Romieu
Anthony
A
Massonneau
Marc
M
Noack-Fraissignes
Pauline
P
Renard
Pierre-Yves
PY
D003432:Cross-Linking Reagents / Q000737:chemistry; D010450:Endopeptidases / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D062385:Hydroxybenzoates / Q000737:chemistry; D015394:Molecular Structure
D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D062385:Hydroxybenzoates; D010450:Endopeptidases; C017616:phenolic acid