Structural identification of anthocyanins and analysis of concentrations during growth and flowering in buckwheat (Fagopyrum esculentum Moench) petals.
The anthocyanin profiles and variety/breeding-line differences of anthocyanin concentrations in petals of common buckwheat flowers have been studied. Four anthocyanins, cyanidin 3-O-glucoside, cyanidin 3-O-rutinoside, cyanidin 3-O-rhamnoside, and cyanidin 3-O-galactosyl-rhamnoside were isolated from the petals of common buckwheat (Fagopyrum esculentum Moench), separated using high performance liquid chromatography and identified using reversed-phase liquid chromatography-electrospray ionization-tandem mass spectrometry techniques. In every variety/breeding line tested, cyanidin 3-O-rutinoside was detected as the major anthocyanin and the next is cyanidin 3-O-glucoside whereas cyanidin 3-O-rhamnoside and cyanidin 3-O-galactosyl-rhamnoside were trace or not detectable in white and pink flowered buckwheat. Of all the varieties/breeding lines tested, Gan-Chao, a Chinese variety, contained the highest amount of anthocyanins. The largest part of cyanidin moiety was presented as a proanthocyanidin form (PAs-Cy). Anthocyanins and PAs-Cy in petals were increased along with increase of flower development stages. Therefore, fully developed petals of red flowered buckwheat, especially Gan-Chao, are promising as a new anthocyanin-rich material for food processing.
Suzuki
Tatsuro
T
National Agricultural and Food Research Organization for Hokkaido Region, Memuro, Hokkaido 082-0071, Japan. tsuzu@affrc.go.jp
Kim
Sun-Ju
SJ
Mohamed
Zaidul Islam Sarker
ZI
Mukasa
Yuji
Y
Takigawa
Shigenobu
S
Matsuura-Endo
Chie
C
Yamauchi
Hiroaki
H
Hashimoto
Naoto
N
Noda
Takahiro
T
Saito
Tatsuya
T
D000872:Anthocyanins / Q000032:analysis* / Q000737:chemistry*; D019612:Fagopyrum / Q000737:chemistry / Q000254:growth & development; D035264:Flowers / Q000737:chemistry* / Q000254:growth & development*
D000872:Anthocyanins; C017154:cyanidin