title: Exploring nociceptive response by BOLD fMRI in alpha-chloralose anesthetized rats.
abstract: The technique of blood oxygenation level dependent (BOLD) functional magnetic resonance imaging (fMRI) was used to provide a spatial-temporal mapping of nociceptive activation in brain. We contrived to obtain an illustration of the pain related regions by injecting formalin at the hindpaw using a 4.7 T MR system in alpha-chloralose anesthetized rats. In order to obtain the pain response, we avoided any invasive surgery on animals to purify the signal of nociception. The dynamic data were analyzed by mapping correlation coefficient and the time activity curves were calculated by atlas-based region of interest selection. The BOLD signals showed obvious difference in anterior cingulated cortex, somatosensory cortex, medial thalamus, and striatum after stimulation. The results not only show the global somatotopic organization of noxious stimulation on hindpaw in rats, but also provided invaluable information for neuroscience research.
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan. f93548021@ntu.edu.tw
authors.forename: Yen-Yu
authors.initials: YY
authors.lastname: Shih
authors.forename: You-Yin
authors.initials: YY
authors.lastname: Chen
authors.forename: Jin-May
authors.initials: JM
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Lung
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Chen
authors.forename: Chen
authors.initials: C
authors.lastname: Chang
authors.forename: Jyh-Cheng
authors.initials: JC
authors.lastname: Chen
authors.forename: Fu-Shan
authors.initials: FS
authors.lastname: Jaw
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000503:physiopathology*; D001931:Brain Mapping / Q000379:methods; D002698:Chloralose / Q000008:administration & dosage*; D005073:Evoked Potentials, Somatosensory; D005557:Formaldehyde; D006993:Hypnotics and Sedatives / Q000008:administration & dosage; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D009619:Nociceptors / Q000187:drug effects; D010101:Oxygen Consumption; D010146:Pain / Q000139:chemically induced* / Q000503:physiopathology*; D010147:Pain Measurement / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006993:Hypnotics and Sedatives; D005557:Formaldehyde; D002698:Chloralose