Hemodynamic, biochemical, and morphological characteristics during preservation of normal porcine livers by hypothermic machine perfusion.
In renal transplantation, hypothermic machine perfusion optimizes preservation of marginal grafts, assesses their quality prior to transplantation, improves outcome, and may contribute to an increased number of transplantations. Recently, hypothermic machine perfusion has become increasingly popular given the organ shortage and the "obligatory" utilization of marginal organs. Increasing mortality on the liver transplantation waiting list makes it urgent to develop machine perfusion systems for livers, trying to better preserve marginal livers and perhaps to recover currently discarded livers are for clinical transplantation without an increased risk of graft nonfunction. However, data on machine perfusion of livers and perfusion parameters capable of predicting viability are scarce. The aim of this study was to determine the baseline hemodynamic and metabolic profiles and morphology of livers during hypothermic machine perfusion in a porcine model. We used protocol similar to hypothermic machine perfusion of kidneys. Hemodynamic analysis revealed higher vascular resistance in the hepatic artery versus the portal vein. The arterial resistance gradually decreased during perfusion (similar to kidneys), suggesting progressive relaxation of the arterial vasculature, and perhaps better penetration of the microcirculation by the perfusion solution. During hypothermic machine perfusion, transaminases were gradually (but modestly) released, and livers displayed unequivocal signs of aerobic and anaerobic metabolism. After 24 hours, livers appeared morphologically well preserved. In conclusion, this study showed that hypothermic machine perfusion was feasible. During hypothermic machine perfusion, was easily assessed hemodynamic, biochemical, and morphological parameters.
Monbaliu
D
D
Abdominal Transplant Surgery Department, University Hospitals Leuven, Herestraat 49, 3000 Leuven, Belgium. Diethard.Monbaliu@uz.kuleuven.ac.be
Vekemans
K
K
De Vos
R
R
Brassil
J
J
Heedfeld
V
V
Qiang
L
L
D'hollander
M
M
Roskams
T
T
Pirenne
J
J
D000818:Animals; D004867:Equipment Design; D006439:Hemodynamics / Q000502:physiology*; D007035:Hypothermia / Q000503:physiopathology; D007036:Hypothermia, Induced / Q000295:instrumentation / Q000379:methods*; D008099:Liver / Q000166:cytology / Q000502:physiology*; D023421:Models, Animal; D009926:Organ Preservation / Q000295:instrumentation / Q000379:methods*; D013552:Swine