title: [Effect of Daming capsule on expression of connexin43 isoforms in hyperlipemic rat's cardiac muscle].
abstract: OBJECTIVE
  To find the molecular mechanism of decreasing blood fat effect of Darning capsule on hyperlipemic rat, we study the expression of connexin43 in the myocardium before and after using the capsule.
  
  
  METHODS
  Forty Wistar rats were randomly divided into 5 group: control group, hyperlipemia model group, Daming capsule group of high dose, middle dose and low dose (200, 100, 50 mg kg(-1) d(-1)). Each group had 8 rats. Hyperlipemic rat model was made firstly, the blood was obtained via vena caudalis and the indexes of TC, TG, LDL, HDL and NEFA in the serum were measured. The myocardial total RNA was extracted by Trizol method. To compare the expression of connexin43 in the following groups: hyperlipemia, normal and drug, we used the technique of RT-PCR, immunostaining and microconfoul.
  
  
  RESULTS
  The concentrations of TC, TG, LDL and NEFA in hyperlipemic serum were increased (P <0. 05), while that of HDL was decreased (P <0. 05). After treating with Daming capsule, the concentration of the preceding four indexes were decreased and the concentrations of HDL was increased up to nearly normal level. No significant difference was found in the ECG of the three groups. As compared with the normal group, the mRNA expressions of connexin43 in hyperlipemia group was weakened (P <0.05), while that of the drug group was enhanced(P <0.05). The same result in immunostaining was observed.
  
  
  CONCLUSIONS
  Hyperlipemic rat model was successfully established and Daming capsule has the effect of lowering blood lipid. Furthermore, the molecular mechanism of Darning capsule is related with the change of Cx43 closely.
authors.affiliation: Department of Pharmacology, Bio-pharmaceutical Key Laboratory of Heilongjiang Province-Incubator of State Key Laboratory, Harbin Medical University, Harbin 150086, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xing
authors.forename: Peng
authors.initials: P
authors.lastname: Yue
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Sun
authors.forename: Wen-xiao
authors.initials: WX
authors.lastname: Zhao
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Yan-jie
authors.initials: YJ
authors.lastname: Lu
authors.forename: Bao-feng
authors.initials: BF
authors.lastname: Yang
mesh_terms: D000818:Animals; D002214:Capsules; D002784:Cholesterol / Q000097:blood; D018031:Connexin 43 / Q000096:biosynthesis* / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D006949:Hyperlipidemias / Q000097:blood / Q000378:metabolism*; D000960:Hypolipidemic Agents / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood
chemical_list: D002214:Capsules; D018031:Connexin 43; D004365:Drugs, Chinese Herbal; D005230:Fatty Acids, Nonesterified; D000960:Hypolipidemic Agents; D020033:Protein Isoforms; D012333:RNA, Messenger; D014280:Triglycerides; D002784:Cholesterol