title: Localization of proteins and organelles using fluorescence microscopy.
abstract: This chapter describes the different methods used for localization of proteins and organelles in Pichia pastoris. A series of plasmids and a modified immunofluorescence protocol for localization and co-localization of proteins and organelles are described. Also included are protocols for the labeling of different subcellular organelles with vital stains.
authors.affiliation: Section of Molecular Biology, Division of Biological Sciences, University of California San Diego, La Jolla, CA, USA.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Farre
authors.forename: Kanae
authors.initials: K
authors.lastname: Shirahama-Noda
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Keith
authors.initials: K
authors.lastname: Booher
authors.forename: Suresh
authors.initials: S
authors.lastname: Subramani
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007211:Indoles; D050296:Microbial Viability; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism; D015388:Organelles / Q000378:metabolism*; D010843:Pichia / Q000166:cytology* / Q000378:metabolism*; D021381:Protein Transport; D011506:Proteins / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism
chemical_list: D007211:Indoles; D011506:Proteins; C007293:DAPI