Macrophage-specific RAM11 monoclonal antibody cross-reacts with basal cells of stratified squamous epithelia.
RAM11 is a mouse monoclonal anti-rabbit macrophage antibody recognizing connective tissue and vascular (atheromatous tissue) macrophages. This study demonstrates a cross-reaction of RAM11 with an unknown antigen in rabbit normal epithelial cells. Formalin-fixed, paraffin sections of the New Zealand White rabbit normal skin, oral mucosa, esophagus, small intestine and lung were immunostained with RAM11 antibody followed by goat anti-mouse Cy-3-conjugated antiglobulin. RAM11-positive immunofluorescence was observed in basal layer cells of stratified squamous epithelia (skin, oral mucosa, esophagus). No RAM11 immunostaining was found in any cells of simple (intestinal, bronchial) epithelia. These findings show that basal cells of stratified squamous keratinized and non-keratinized epithelia of the rabbit express an antigenic epitope which is common with that of macrophage antigen recognized by RAM11 monoclonal antibody.
Lis
Grzegorz J
GJ
Department of Histology, Jagiellonian University Medical College, Krakow, Poland. mmlis@cyf-kr.edu.pl
Litwin
Jan A
JA
Furgal-Borzych
Alicja
A
Zarzecka
Joanna
J
Cichocki
Tadeusz
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000032:analysis; D003429:Cross Reactions; D004847:Epithelial Cells / Q000145:classification / Q000166:cytology* / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D011817:Rabbits
D000911:Antibodies, Monoclonal; D000941:Antigens