Inhibitory effects of selenium on telomerase activity and hTERT expression in cadmium-transformed 16HBE cells.
OBJECTIVE
To investigate the effects of sodium selenite on telomerase activity and expression of hTERT mRNA in cadmium-transformed 16HBE cells.


METHODS
Telomerase activity and expression of genes were measured after cultured cadmium-transformed 16HBE cells were exposed to sodium selenite at different doses (0.625, 1.25, 2.50, 5.00 micromol/L) for 24 hours.


RESULTS
Selenium decreased telomerase activity in cadmium-transformed 16HBE cells. There existed an obvious dose-effect relationship between the selenium concentration and these changes. The expression of hTERT and c-myc mRNA also decreased but the expression of mad1 mRNA increased after exposure to selenium for 24 hours. No difference was found in expression of hTRF1 and hTRF2 mRNA after incubated with sodium selenite for 24 hours, compared with control group.


CONCLUSIONS
Selenium inhibits telomerase activity by decreasing hTERT and c-myc mRNA expression and increasing mad1 mRNA expression in cadmium-transformed 16HBE cells and selenium concentration is significantly correlated with these changes.
Chen
Hua-Jie
HJ
Department of Occupational and Environmental Health, MOE Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
Yu
Ri-An
RA
He
Ling-Fei
LF
An
She-Juan
SJ
Wu
Zhi-Gang
ZG
Yang
Ke-Di
KD
Chen
Xue-Min
XM
D001483:Base Sequence; D002104:Cadmium / Q000494:pharmacology*; D002461:Cell Line, Transformed; D017931:DNA Primers; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018038:Sodium Selenite / Q000494:pharmacology*; D019098:Telomerase / Q000037:antagonists & inhibitors* / Q000235:genetics
D017931:DNA Primers; D012333:RNA, Messenger; D002104:Cadmium; C509186:TERT protein, human; D019098:Telomerase; D018038:Sodium Selenite