title: Caspase-2 cleaves DNA fragmentation factor (DFF45)/inhibitor of caspase-activated DNase (ICAD).
abstract: To investigate the signal transduction pathway of caspase-2, cell permeable Tat-reverse-caspase-2 was constructed, characterized and utilized for biochemical and cellular studies. It could induce the cell death as early as 2h, and caspase-2-specific VDVADase activity but not other caspase activities including DEVDase and IETDase. Interestingly, nuclear DNA fragmentation occurred and consistently DNA fragmentation factor (DFF45)/Inhibitor of caspase-activated DNase (ICAD) was cleaved inside the cell as well as in vitro, suggesting a role of caspase-2 in nuclear DNA fragmentation.
authors.affiliation: Research Center for Proteineous Materials (RCPM), Department of Bio-materials Engineering, Chosun University, Gwangju 501-759, Republic of Korea.
authors.forename: Giri Raj
authors.initials: GR
authors.lastname: Dahal
authors.forename: Pratap
authors.initials: P
authors.lastname: Karki
authors.forename: Arjun
authors.initials: A
authors.lastname: Thapa
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shahnawaz
authors.forename: Song Yub
authors.initials: SY
authors.lastname: Shin
authors.forename: Jung Sup
authors.initials: JS
authors.lastname: Lee
authors.forename: Byungyun
authors.initials: B
authors.lastname: Cho
authors.forename: Il-Seon
authors.initials: IS
authors.lastname: Park
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D053143:Caspase 2 / Q000378:metabolism*; D002470:Cell Survival / Q000502:physiology*; D004789:Enzyme Activation; D006367:HeLa Cells; D006801:Humans; D011506:Proteins / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D011506:Proteins; C109664:caspase-activated DNase inhibitor; D053143:Caspase 2