title: Interrogation of G-quadruplex-protein interactions.
abstract: The ability to accurately examine the interaction of G-quadruplex DNA with proteins is essential for revealing the biological roles of these unusual DNA structures. In this regard, there are four primary G-quadruplex-related activities of proteins that have been studied including simple equilibrium binding, promotion or catalysis of G-quadruplex formation, dissociation of G-quadruplex structures, and covalent modification of G-quadruplexes, which includes both nucleolytic cleavage and nucleotide addition. Here, assays used to examine the interactions of G-quadruplexes with proteins will be reviewed and specific methods to study the interactions of G-quadruplexes from telomeric DNA sequences with a variety of proteins will be described. Importantly, this review emphasizes the importance of evaluating the integrity of the G-quadruplex being studied as single sequences can often form a variety of folded structures.
authors.affiliation: Children's Medical Research Institute, 214 Hawkesbury Road, Westmead, Sydney, NSW 2145, Australia. tbryan@cmri.usyd.edu.an
authors.forename: Tracy M
authors.initials: TM
authors.lastname: Bryan
authors.forename: Michael B
authors.initials: MB
authors.lastname: Jarstfer
mesh_terms: D004247:DNA / Q000737:chemistry*; D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins; D006147:Guanine; D004247:DNA