title: Strategies to evade false positives in the in situ analysis of peptide antibiotics in SDS-PAGE gels.
abstract: In situ activity assay is one of the promising techniques for the characterization of peptide antibiotics. This assay was carried out for the peptide purified from a new bacterial isolate Paenibacillus alvei and commercial peptide antibiotic polymixin E. Towards this, the routine and new protocols were tried. Interestingly, the unexpected result of these experiments - the "switch over activity" has led us to have further investigations. Here, we have addressed the potential problem in the methodology of in situ assay and demonstrated a fool proof protocol to evade the false positive results.
authors.affiliation: Centre for Biotechnology, Genetic Engineering Unit, Anna University, Chennai 600025, India.
authors.forename: Balaiah
authors.initials: B
authors.lastname: Anandaraj
authors.forename: Athinarayanan
authors.initials: A
authors.lastname: Selvamanikandan
authors.forename: Prabakar
authors.initials: P
authors.lastname: Mittal
authors.forename: Natarajan
authors.initials: N
authors.lastname: Elavarasi
authors.forename: Chinnathambi
authors.initials: C
authors.lastname: Thangadurai
authors.forename: Vadivel
authors.initials: V
authors.lastname: Murugan
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D003091:Colistin / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005189:False Positive Reactions; D006090:Gram-Negative Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010455:Peptides / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides; D003091:Colistin