Anti-thyroperoxidase antibodies from patients with Hashimoto's encephalopathy bind to cerebellar astrocytes.
A cohort of 10 Hashimoto's encephalopathy (HE) patients, 33 patients with unrelated neurological symptoms, 12 Hashimoto's thyroiditis patients and 4 healthy adult donors was studied to explore the neurological targets of anti-thyroperoxidase (TPO) autoantibodies (aAb) in HE. High levels of anti-TPO aAb were only detected in HE group's cerebrospinal fluids. In immunofluorescence assays on monkey brain cerebellum sections, both HE patients' sera and anti-TPO monoclonal antibodies (mAb) were able to bind cerebellar cells expressing glial fibrillary acid protein. Normal human astrocytes from primary cultures also reacted with anti-TPO mAb. Specific astrocyte binding of anti-TPO aAb suggests a role of these aAb in the HE pathogenesis.
Blanchin
Stéphanie
S
Laboratoire d'Immunologie et d'Immunopathologie, UPRES-EA 2128, CHU Clémenceau, 14033 Caen cedex, France. blanchin-s@chu-caen.fr
Coffin
Christine
C
Viader
Fausto
F
Ruf
Jean
J
Carayon
Pierre
P
Potier
Francette
F
Portier
Estelle
E
Comby
Elisabeth
E
Allouche
Stéphane
S
Ollivier
Yann
Y
Reznik
Yves
Y
Ballet
Jean Jacques
JJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D001253:Astrocytes / Q000378:metabolism*; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D002478:Cells, Cultured; D002531:Cerebellum / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005333:Fetus; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D050031:Hashimoto Disease / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007453:Iodide Peroxidase / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D013954:Thyroglobulin / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology*; D013961:Thyroid Gland / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
D001323:Autoantibodies; D005904:Glial Fibrillary Acidic Protein; D013954:Thyroglobulin; D007453:Iodide Peroxidase