Reciprocal endoderm-mesoderm interactions mediated by fgf24 and fgf10 govern pancreas development.
In amniotes, the pancreatic mesenchyme plays a crucial role in pancreatic epithelium growth, notably through the secretion of fibroblast growth factors. However, the factors involved in the formation of the pancreatic mesenchyme are still largely unknown. In this study, we characterize, in zebrafish embryos, the pancreatic lateral plate mesoderm, which is located adjacent to the ventral pancreatic bud and is essential for its specification and growth. We firstly show that the endoderm, by expressing the fgf24 gene at early stages, triggers the patterning of the pancreatic lateral plate mesoderm. Based on the expression of isl1, fgf10 and meis genes, this tissue is analogous to the murine pancreatic mesenchyme. Secondly, Fgf10 acts redundantly with Fgf24 in the pancreatic lateral plate mesoderm and they are both required to specify the ventral pancreas. Our results unveil sequential signaling between the endoderm and mesoderm that is critical for the specification and growth of the ventral pancreas, and explain why the zebrafish ventral pancreatic bud generates the whole exocrine tissue.
Manfroid
Isabelle
I
GIGA-Research-Unité de Biologie Moléculaire et Génie Génétique, Tour B34, Université de Liège, B-4000 Sart Tilman, Belgium. isabelle.manfroid@ulg.ac.be
Delporte
François
F
Baudhuin
Ariane
A
Motte
Patrick
P
Neumann
Carl J
CJ
Voz
Marianne L
ML
Martial
Joseph A
JA
Peers
Bernard
B
D000818:Animals; D030801:Animals, Genetically Modified; D002450:Cell Communication / Q000502:physiology; D002454:Cell Differentiation; D004625:Embryo, Nonmammalian; D004707:Endoderm / Q000378:metabolism / Q000502:physiology*; D051526:Fibroblast Growth Factor 10; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D060850:LIM-Homeodomain Proteins; D008648:Mesoderm / Q000378:metabolism / Q000502:physiology*; D008954:Models, Biological; D009928:Organ Specificity; D010179:Pancreas / Q000196:embryology* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors; D015027:Zebrafish / Q000196:embryology; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D051526:Fibroblast Growth Factor 10; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; C102642:Meis3 protein, zebrafish; D014157:Transcription Factors; D029961:Zebrafish Proteins; C520413:fgf10a protein, zebrafish; C479567:fibroblast growth factor 24, zebrafish; C063843:insulin gene enhancer binding protein Isl-1; D005346:Fibroblast Growth Factors