Most MCF7 and SK-OV3 cells were deprived of their stem nature by Hoechst 33342.
Using clonal and population analysis, we found that the MCF7 and SK-OV3 were composed mainly of cancer stem cells. Together with SP analysis, we found that both SP and NSP cells of MCF7 and SK-OV3 contained different proportions of cancer stem cells. In addition, we discovered that MCF7 SP cells were able to generate non-SP cells, and conversely non-SP cells generated SP cells; Moreover, we showed that Hoechst 33342 and FACS were harmful to the clonogenicity of MCF7 and SK-OV3 cells.
Zhong
Yong
Y
Department of Immunology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Pan Jia Yuan Nan Li, Chao Yang District, Beijing 100021, China.
Zhou
Chunxia
C
Ma
Wenbo
W
Wang
Dongmei
D
Guo
Sujuan
S
Su
Xiaosan
X
Zhang
Shuren
S
D001562:Benzimidazoles / Q000494:pharmacology*; D045744:Cell Line, Tumor; D019070:Cell Lineage; D002469:Cell Separation; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006801:Humans; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000187:drug effects*
D001562:Benzimidazoles; D005456:Fluorescent Dyes; C017807:bisbenzimide ethoxide trihydrochloride