[Expression in Escherichia coli of the gspd(l) gene of the type II secretion system in Leptospira borgpetersenii serovariety hardjo].
A fragment of 1.539 pb of the gene homologous to gspD of Hardjobovis was clonated in the pET28a vector and it was transformed into E. coli C43 and Rosetta strains. The sequence of GspD(L) showed 46 % of similitude with E. coli GspD secretin. The expression of recombinant GspD(L) was obtained in Rosetta strain.
Reyes
Ernesto Armando Rodríguez
EA
Facultad de Medicina Veterinaria y Zootecnia, UNAM, México.
Cullen
Paul
P
Bulach
Dieter
D
Adler
Ben
B
Haake
David
D
de la Peña-Moctezuma
Alejandro
A
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D006801:Humans; D007919:Leptospira / Q000235:genetics* / Q000276:immunology; D007922:Leptospirosis / Q000235:genetics* / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D014169:Transformation, Bacterial
D029968:Escherichia coli Proteins