[The differential expression of the genes of the key enzymes involved in phenolic compound metabolism in rice (Oryza sativa L.) under different nitrogen supply].
Differential expression of the key genes controlling phenolic metabolism in allelopathic and non-allelopathic rice accessions was investigated under two nitrogen supply levels (lower and normal) using fluorescence quantitative-polymerase chain reaction (FQ-PCR) (Figs.2, 3). The results indicated that 9 key enzyme genes concerned were mediated by lower nitrogen level (Table 2). All of the nine genes (Table 1, Fig.4), were up-regulated by 1.9-5.4 times of the relative gene expression amounts in allelopathic rice accession, 'PI312777' under the lower nitrogen condition compared with their controls, of which PAL gene showed the highest relative gene expression amount with 5.4 times of the relative gene expressions compared with the control, while in non-allelopathic rice Lemont, seven genes were down-regulated by 29%-72% under lower nitrogen supplies compared with their controls and only two genes, i.e., phenylalanine ammonia-lyase and cinnamoyl-CoA genes were up-regulated, which however were a decrease of 22% and 74% over those in allelopathic rice accession (Table 2). These findings strongly suggest that the increase of allelopathic potential induced by 1/4 nutrient stress was responsible for enhanced phenolic compound synthesis metabolism.
Xiong
Jun
J
School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Wang
Hai-Bin
HB
Fang
Chang-Xun
CX
Qiu
Long
L
Wu
Wen-Xiang
WX
He
Hai-Bin
HB
Lin
Wen-Xiong
WX
D000214:Acyl Coenzyme A / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D008954:Models, Biological; D009584:Nitrogen / Q000378:metabolism*; D012275:Oryza / Q000201:enzymology* / Q000235:genetics*; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics; D016133:Polymerase Chain Reaction
D000214:Acyl Coenzyme A; C022903:cinnamoyl-coenzyme A; D010650:Phenylalanine Ammonia-Lyase; D009584:Nitrogen