Microfabrication procedure of PDMS microbeam array using photolithography for laminin printing and piconewton force transduction on axons.
The purpose of this paper is to introduce our design for transducing forces on the order of tens of piconewtons by optically measuring deflection of a microfabricated beam tip as it pulls on an array of flexible structures such as axons in an array of laminin-printed neurons. To achieve this we have designed polymeric beams with spring constants on the order of 10 pN/microm. We have fabricated circular microbeams with Sylgard polydimethylsiloxane (PDMS). The elastic modulus of PDMS was determined experimentally using a microscale and a micrometer at different concentrations of curing agent and base agent and found to be on the order of 100 kPa. The designed geometry is a 100x100 tapered microcone array with each beam having a length of 100 microm, and a base diameter of 10 microm. A SU-8 negative photoresist is etched using photolithography and used as a mold for PDMS soft lithography. PDMS was injected into the mold and the array peeled from the mold.
Sasoglu
F Mert
FM
Drexel University, Philadelphia, PA 19104, USA.
Bohl
Andrew J
AJ
Layton
Bradley E
BE
D001369:Axons / Q000502:physiology*; D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007797:Laminin / Q000737:chemistry*; D008365:Manometry / Q000295:instrumentation* / Q000379:methods; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D046228:Microarray Analysis / Q000295:instrumentation*; D008904:Miniaturization; D009757:Nylons / Q000737:chemistry*; D010781:Photography / Q000379:methods*; D013314:Stress, Mechanical; D014159:Transducers
D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; D007797:Laminin; D009757:Nylons; C085707:poly(dimethylsiloxane)-polyamide copolymer