A quantitative and qualitative method for direct 2-DE analysis of murine cartilage.
Direct 2-DE analysis of cartilage is difficult due to the high proteoglycan content. Proteoglycan removal before IEF may however cause the partial or total loss of specific proteins making this approach ineffective when quantitative data are required to investigate protein expression differences. Thus, we have developed a 2-DE method including passive rehydration loading that does not require sample pretreatment and allows direct protein expression studies in cartilage samples.
Pecora
Fabio
F
Dipartimento di Biochimica Alessandro Castellani, Università di Pavia, Pavia, Italy.
Forlino
Antonella
A
Gualeni
Benedetta
B
Lupi
Anna
A
Giorgetti
Sofia
S
Marchese
Loredana
L
Stoppini
Monica
M
Tenni
Ruggero
R
Cetta
Giuseppe
G
Rossi
Antonio
A
D000818:Animals; D000831:Animals, Newborn; D002358:Cartilage, Articular / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D040081:Protein Array Analysis; D020543:Proteome / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results
D020543:Proteome