title: Permuted tRNA genes expressed via a circular RNA intermediate in Cyanidioschyzon merolae.
abstract: A computational analysis of the nuclear genome of a red alga, Cyanidioschyzon merolae, identified 11 transfer RNA (tRNA) genes in which the 3' half of the tRNA lies upstream of the 5' half in the genome. We verified that these genes are expressed and produce mature tRNAs that are aminoacylated. Analysis of tRNA-processing intermediates for these genes indicates an unusual processing pathway in which the termini of the tRNA precursor are ligated, resulting in formation of a characteristic circular RNA intermediate that is then processed at the acceptor stem to generate the correct termini.
authors.affiliation: Department of Life Science, College of Science, Rikkyo (St. Paul's) University, Toshima, Tokyo 171-8501, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Soma
authors.forename: Akinori
authors.initials: A
authors.lastname: Onodera
authors.forename: Junichi
authors.initials: J
authors.lastname: Sugahara
authors.forename: Akio
authors.initials: A
authors.lastname: Kanai
authors.forename: Nozomu
authors.initials: N
authors.lastname: Yachie
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
authors.forename: Fujio
authors.initials: F
authors.lastname: Kawamura
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Sekine
mesh_terms: D001483:Base Sequence; D048028:DNA, Algal / Q000737:chemistry / Q000235:genetics; D005796:Genes; D008718:Methionine-tRNA Ligase / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D048030:RNA, Algal / Q000235:genetics* / Q000378:metabolism; D000079962:RNA, Circular; D012343:RNA, Transfer / Q000235:genetics* / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D000461:Rhodophyta / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D048028:DNA, Algal; D048030:RNA, Algal; D000079962:RNA, Circular; D012346:RNA, Transfer, Amino Acyl; D012313:RNA; D012343:RNA, Transfer; D008718:Methionine-tRNA Ligase