Phospholipase A(1) assays using a radiolabeled substrate and mass spectrometry.
Although a number of phospholipase A(1)s (PLA(1)s) have been identified in the recent decade, the physiological functions of PLA(1)s remain almost elusive. The major reason for this is the poor availability of assay methods. In many studies, radiolabeled phospholipid substrates have been used to measure PLA(1) activity. This chapter describes the conventional PLA(1) assay using a radiolabeled substrate and a novel PLA(1) assay using electrospray ionization mass spectrometry.
Morikawa
Rei
R
Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Tsujimoto
Masafumi
M
Arai
Hiroyuki
H
Aoki
Junken
J
D000818:Animals; D002250:Carbon Radioisotopes; D017815:Elapidae; D006367:HeLa Cells; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D007553:Isotope Labeling / Q000379:methods; D008322:Mammals; D013058:Mass Spectrometry; D054466:Phospholipases A1 / Q000032:analysis*; D054467:Phospholipases A2 / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D002250:Carbon Radioisotopes; D007527:Isoenzymes; D010743:Phospholipids; D011994:Recombinant Proteins; D054466:Phospholipases A1; D054467:Phospholipases A2