title: [Detecting VA, VD3, VE and beta-carotene with HPLC in human serums].
abstract: OBJECTIVE
  To develop a method of detecting retinol (vitamin A), vitamin D3, alpha-tocopherol (vitamin E) and beta-carotene in human serums with HPLC.
  
  
  METHODS
  Proteins were precipitated with anhydrous ethanol. Fat-solutable vitamins in human serums were extracted with aether/petroleum ether mixture and determined with HPLC.
  
  
  RESULTS
  The linear ranges for the retinol, VD3, alpha-tocopherol and beta-carotene were 0.012 microg/mL-500 microg/mL, 0.030 microg/mL-500 microg/mL, 0.12 microg/mL-500 microg/mL, and 0.015 microg/mL-500 microg/mL, respectively. The detection limits for the retinol, VD3, alpha-tocopherol and beta-carotene were 0.012 microg/mL, 0.030 microg/mL, 0.12 microg/mL and 0.015 microg/mL, respectively. The relative standard derivations (RSD) for the retinol, VD3, alpha-tocopherol and beta-carotene were 0.75%, 0.54%, 2.06% and 2.74%, respectively. The proposed method recovered 92%-116%, 98%-112%, 84.8%-106%, and 90%-105% retinol, VD3, alpha-tocopherol and beta-carotene in human serums respectively.
  
  
  CONCLUSIONS
  The method is simple, quick and applicable to all of the four fat-solutable vitamins.
authors.affiliation: Department of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Zou
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Zeng
authors.forename: Yuan-qian
authors.initials: YQ
authors.lastname: Li
authors.forename: Xiao-xia
authors.initials: XX
authors.lastname: Li
authors.forename: Si-wei
authors.initials: SW
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
authors.forename: Cheng-yu
authors.initials: CY
authors.lastname: Huang
mesh_terms: D002762:Cholecalciferol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D014801:Vitamin A / Q000032:analysis*; D024502:alpha-Tocopherol / Q000032:analysis*; D019207:beta Carotene / Q000032:analysis*
chemical_list: D019207:beta Carotene; D014801:Vitamin A; D002762:Cholecalciferol; D024502:alpha-Tocopherol