title: [Drug susceptibility of Haemophilus sp. and Staphylococcus aureus strains colonizing respiratory tract in children with mucoviscidosis].
abstract: OBJECTIVE
  The aim of our studies was to isolate haemophili rods and S. aureus from children with mucoviscidosis and to assess antimicrobial agent susceptibility of the isolates.
  
  
  METHODS
  Sputa and nasopharynx swabs from 12 children with mucoviscidosis were screened for the prevalence ofHaemophilus sp. and S. aureus. Susceptibility to antimicrobial agents was performed by agar diffusion method using appropriate media. Beta-lactamase activity was determined with the nitrocefin discs, macrolide-lincosamide-streptogramin B resistance using D-test.
  
  
  METHODS
  Of 12 mucoviscidosis-positive patients, 7 (58.33%) were colonisated by haemophili rods (a total of 8 H. parainfluenzae, 2 H. influenzae or 2 Haemophilus sp. isolates) and by S. aureus (8 isolates), in these species were isolated alone or in mixed cultures. Two ampicillin-resistant Haemophilus sp. but without beta-lactamases production were obtained. Also two methicillin resistant S. aureus with constitutive phenotype of resistance to macrolides-lincosamides and streptogramins B isolates and two methicillin-sensitive (with constitutive and inducible resistance to macrolides-lincosamides and streptogramins B) were isolated.
authors.affiliation: Katedra i Zakład Mikrobiologii Farmaceutycznej, Akademii Medycznej im. prof. F. Skubiszewskiego w Lublinie. u.kosikowska@am.lublin.pl
authors.forename: Urszula
authors.initials: U
authors.lastname: Kosikowska
authors.forename: Marek
authors.initials: M
authors.lastname: Juda
authors.forename: Anna
authors.initials: A
authors.lastname: Malm
authors.forename: Marta
authors.initials: M
authors.lastname: Jóźwiakowska
authors.forename: Ewa
authors.initials: E
authors.lastname: Tuszkiewicz-Misztal
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D002648:Child; D003550:Cystic Fibrosis / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D006190:Haemophilus / Q000187:drug effects* / Q000302:isolation & purification; D006193:Haemophilus influenzae / Q000187:drug effects; D044135:Haemophilus parainfluenzae / Q000187:drug effects; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D012137:Respiratory System / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000890:Anti-Infective Agents