Stability-indicating method for determination of oxyphenonium bromide and its degradation product by high-performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) method was developed for determination of oxyphenonium bromide (OX) and its degradation product. The method was based on the HPLC separation of OX from its degradation product, using a cyanopropyl column at ambient temperature with mobile phase of acetonitrile-25 mM potassium dihydrogen phosphate, pH 3.4 (50 + 50, v/v). UV detection at 222 nm was used for quantitation based on peak area. The method was applied to the determination of OX and its degradation product in tablets. The proposed method was also used to investigate the kinetics of the acidic and alkaline degradation of OX at different temperatures, and the apparent pseudo first-order rate constant, half-life, and activation energy were calculated. The pH-rate profile of the degradation of OX in Britton-Robinson buffer solutions within the pH range 2-12 was studied.
El-Gindy
Alaa
A
Suez Canal University, Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Ismailia 41522, Egypt. chemomet78@yahoo.com
Emara
Samy
S
Shaaban
Heba
H
D000097:Acetonitriles / Q000737:chemistry; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D016014:Linear Models; D008956:Models, Chemical; D010115:Oxyphenonium / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013607:Tablets; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature
D000097:Acetonitriles; D013607:Tablets; D010115:Oxyphenonium; C032159:acetonitrile