title: Metabolic engineering of Escherichia coli for 1-butanol production.
abstract: Compared to ethanol, butanol offers many advantages as a substitute for gasoline because of higher energy content and higher hydrophobicity. Typically, 1-butanol is produced by Clostridium in a mixed-product fermentation. To facilitate strain improvement for specificity and productivity, we engineered a synthetic pathway in Escherichia coli and demonstrated the production of 1-butanol from this non-native user-friendly host. Alternative genes and competing pathway deletions were evaluated for 1-butanol production. Results show promise for using E. coli for 1-butanol production.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA 90095, USA.
authors.forename: Shota
authors.initials: S
authors.lastname: Atsumi
authors.forename: Anthony F
authors.initials: AF
authors.lastname: Cann
authors.forename: Michael R
authors.initials: MR
authors.lastname: Connor
authors.forename: Claire R
authors.initials: CR
authors.lastname: Shen
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Smith
authors.forename: Mark P
authors.initials: MP
authors.lastname: Brynildsen
authors.forename: Katherine J Y
authors.initials: KJ
authors.lastname: Chou
authors.forename: Taizo
authors.initials: T
authors.lastname: Hanai
authors.forename: James C
authors.initials: JC
authors.lastname: Liao
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D001674:Bioelectric Energy Sources; D046969:Clostridium acetobutylicum / Q000502:physiology*; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D020001:1-Butanol