title: Optimization and validation of a high-performance liquid chromatographic method with UV detection for the determination of pyrrole-imidazole polyamides in rat plasma.
abstract: A simple and sensitive high-performance liquid chromatography (HPLC) method utilizing UV detection was developed for the determination of plasma pyrrole (Py)-imidazole (Im) polyamides in rats and applied to the pharmacokinetic study of compounds. After deproteinization of plasma with methanol, Py-Im polyamides were analyzed with a reversed-phase TSK-GEL ODS-80TM (4.6 mmx15.0 cm TOSOH Co., Japan) column maintained at 40 degrees C. The mobile phase solvent A was 0.1% acetic acid and the solvent B was HPLC-grade acetonitrile (0-10 min, A: 100-20%, B: 0-80% linear gradient; 10-15 min, A: 40%, B: 60%). The flow rate was 1.0 ml/min. The detection wavelength was set at 310 nm. The method was used to determine the plasma concentration time profiles of Py-Im polyamides after intravenous injection.
authors.affiliation: Department of Clinical Pharmacokinetics, College of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi, Chiba 274-8555, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Fukasawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagashima
authors.forename: Takahiko
authors.initials: T
authors.lastname: Aoyama
authors.forename: Noboru
authors.initials: N
authors.lastname: Fukuda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Matsuda
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ueno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Hiroki
authors.initials: H
authors.lastname: Nagase
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007093:Imidazoles / Q000097:blood*; D008297:Male; D009757:Nylons / Q000032:analysis*; D011758:Pyrroles / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays
chemical_list: D007093:Imidazoles; D009757:Nylons; D011758:Pyrroles