title: Electric field effects on human spinal injury: Is there a basis in the in vitro studies?
abstract: An important basis for the clinical application of small DC electric current to mammalian spinal injury is the responses of neurons in culture to applied electric fields. Our recent finding that zebrafish neurons were unresponsive to applied fields prompted us to critically examine previous results. We conclude that compelling evidence for neuronal guidance and directional stimulation of growth toward either the cathode or anode in an electric field exists only for cultured Xenopus neurons, and not for any mammalian neurons. No basis for the reported success in treating spinal injury exists in the in vitro studies, and considerable research will be required if the conditions of field application in mammalian spinal injury are to be optimized.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA. robinsok@purdue.edu
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Robinson
authors.forename: Peter
authors.initials: P
authors.lastname: Cormie
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004599:Electric Stimulation Therapy / Q000639:trends*; D004574:Electromagnetic Fields; D020439:Growth Cones / Q000502:physiology / Q000528:radiation effects; D006801:Humans; D023421:Models, Animal; D009416:Nerve Regeneration / Q000502:physiology / Q000528:radiation effects*; D013045:Species Specificity; D013116:Spinal Cord / Q000502:physiology / Q000528:radiation effects*; D013119:Spinal Cord Injuries / Q000503:physiopathology / Q000628:therapy*; D014982:Xenopus laevis / Q000502:physiology