[Cloning NS1 gene of H5N1 avian influenza virus and apoptosis induced by it in human pulmonary carcinoma cell line A549].
The NS1 gene of the H5N1 subtype avian influenza virus was amplified by RT-PCR, and the am-plified product was cloned into the eukaryotic expression vector pCMV-Myc, then it was transfected into A549 cells. After 48 h, the expression of NS1 was detected by Western blot. Fluorescence and electron microscopy and flow cytometry showed that the NS1 gene of H5N1 avian influenza virus could induce apop-tosis in human pulmonary carcinoma cell line A549.
Zhang
Chuan-Fu
CF
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. hnzcf@126.com
Jiang
Shi-Wei
SW
Zhu
Heng-Qi
HQ
Yang
Yu-Tao
YT
Yang
Zhi-Xin
ZX
Xu
Long
L
Zhao
Li-Xia
LX
Zhou
Xiao-Wei
XW
Huang
Pei-Tang
PT
D017304:Annexin A5 / Q000032:analysis; D017209:Apoptosis; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000472:pathogenicity; D008175:Lung Neoplasms / Q000473:pathology; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000502:physiology
D017304:Annexin A5; C085820:INS1 protein, influenza virus; D017361:Viral Nonstructural Proteins