Fluorescence microscopy to study pressure between lipids in giant unilamellar vesicles.
The authors developed a technique to apply high hydrostatic pressure to giant unilamellar vesicles and to directly observe the consequent structural changes with two-photon fluorescence microscopy imaging using high numerical aperture oil-immersion objectives. The data demonstrate that high pressure has a dramatic effect on the shape of the vesicles, and both fluidity and homogeneity of the membrane.
Celli
Anna
A
Department of Physics, University of Illinois, Urbana-Champaign, USA.
Lee
Claudia Y C
CY
Gratton
Enrico
E
D015081:2-Naphthylamine / Q000031:analogs & derivatives* / Q000737:chemistry; D006874:Hydrostatic Pressure; D007848:Laurates / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D036641:Microscopy, Fluorescence, Multiphoton; D010713:Phosphatidylcholines / Q000737:chemistry*
D007848:Laurates; D008081:Liposomes; D010713:Phosphatidylcholines; D015081:2-Naphthylamine; C065580:laurdan