title: Evidence for the presence of Legionella bacteriophages in environmental water samples.
abstract: The existence and preliminary characterization of bacteriophages active against the Gram-negative human pathogen Legionella pneumophila, the causative agent of a very severe form of pneumonia, are reported. Four phages belonging to the family of the Myoviridae were isolated from various fresh water environments, and preliminary characterization showed that these crude preparations infect exclusively bacteria belonging to the genus Legionella. Standard phage amplification, purification, and characterization procedures were, however, not efficiently applicable making more research into these novel phages and their mechanism of infection necessary. The existence of Legionella bacteriophages is very promising for future applications such as the development of novel molecular tools, the design of new detection and typing methods, and the bioremediation of this environmental pathogen.
authors.affiliation: Laboratory for Bacteriology, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, Leuven, Belgium. Elke.Lammertyn@rega.kuleuven.be
authors.forename: Elke
authors.initials: E
authors.lastname: Lammertyn
authors.forename: Johan
authors.initials: J
authors.lastname: Vande Voorde
authors.forename: Eef
authors.initials: E
authors.lastname: Meyen
authors.forename: Liesbeth
authors.initials: L
authors.lastname: Maes
authors.forename: Jan
authors.initials: J
authors.lastname: Mast
authors.forename: Jozef
authors.initials: J
authors.lastname: Anné
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D003470:Culture Media; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D005618:Fresh Water / Q000821:virology*; D006801:Humans; D007875:Legionella / Q000145:classification / Q000821:virology*; D016952:Legionella pneumophila / Q000821:virology*; D008242:Lysogeny; D017900:Myoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D013045:Species Specificity
chemical_list: D003470:Culture Media; D004279:DNA, Viral