Arp2/3-independent assembly of actin by Vibrio type III effector VopL.
Microbial pathogens use a variety of mechanisms to disrupt the actin cytoskeleton during infection. Vibrio parahaemolyticus (V. para) is a Gram-negative bacterium that causes gastroenteritis, and new pandemic strains are emerging throughout the world. Analysis of the V. para genome revealed a type III secretion system effector, VopL, encoding three Wiskott-Aldrich homology 2 domains that are interspersed with three proline-rich motifs. Infection of HeLa cells with V. para induces the formation of long actin fibers in a VopL-dependent manner. Transfection of VopL promotes the assembly of actin stress fibers. In vitro, recombinant VopL potently induces assembly of actin filaments that grow at their barbed ends, independent of eukaryotic factors. Vibrio VopL is predicted to be a bacterial virulence factor that disrupts actin homeostasis during an enteric infection of the host.
Liverman
Amy D B
AD
Department of Molecular Biology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA.
Cheng
Hui-Chun
HC
Trosky
Jennifer E
JE
Leung
Daisy W
DW
Yarbrough
Melanie L
ML
Burdette
Dara L
DL
Rosen
Michael K
MK
Orth
Kim
K
D051376:Actin-Related Protein 2-3 Complex / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D005799:Genes, Dominant; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D041681:NIH 3T3 Cells; D010455:Peptides / Q000378:metabolism; D055232:Proline-Rich Protein Domains; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000378:metabolism; D022502:Stress Fibers / Q000378:metabolism; D014162:Transfection; D014735:Vibrio Infections; D014736:Vibrio parahaemolyticus / Q000378:metabolism* / Q000472:pathogenicity; D037521:Virulence Factors / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D001426:Bacterial Proteins; D010455:Peptides; D011994:Recombinant Proteins; D037521:Virulence Factors; D020742:rhoA GTP-Binding Protein