Transport of carnitine and acetylcarnitine by carnitine/organic cation transporter (OCTN) 2 and OCTN3 into epididymal spermatozoa.
Carnitine and acetylcarnitine are important for the acquisition of motility and maturation of spermatozoa in the epididymis. In this study, we examined the involvement of carnitine/organic cation transporter (OCTN) in carnitine and acetylcarnitine transport in epididymal spermatozoa of mice. Uptake of both compounds by epididymal spermatozoa was time-dependent and partially Na(+)-dependent. Kinetic analyses revealed the presence of a high-affinity transport system in the spermatozoa, with K(m) values of 23.6 and 6.57 muM for carnitine and acetylcarnitine respectively in the presence of Na(+). Expression of OCTN2 and OCTN3 in epididymal spermatozoa was confirmed by immunofluorescence analysis. The involvement of these two transporters in carnitine and acetylcarnitine transport was supported by a selective inhibition study. We conclude that both Na(+)-dependent and -independent carnitine transporters, OCTN2 and OCTN3, mediate the supply of carnitine and acetylcarnitine to epididymal spermatozoa in mice.
Kobayashi
Daisuke
D
Division of Pharmaceutical Sciences, Department of Molecular Biopharmaceutics, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
Tamai
Ikumi
I
Sai
Yoshimichi
Y
Yoshida
Kazuhiro
K
Wakayama
Tomohiko
T
Kido
Yasuto
Y
Nezu
Jun-Ichi
J
Iseki
Shoichi
S
Tsuji
Akira
A
D000108:Acetylcarnitine / Q000493:pharmacokinetics*; D000818:Animals; D001622:Betaine / Q000031:analogs & derivatives / Q000494:pharmacology; D001693:Biological Transport, Active; D002331:Carnitine / Q000493:pharmacokinetics* / Q000494:pharmacology; D004822:Epididymis; D005455:Fluorescent Antibody Technique; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051379:Mice; D027701:Organic Cation Transport Proteins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011738:Pyrilamine / Q000494:pharmacology; D000074058:Solute Carrier Family 22 Member 5; D013079:Sperm Maturation / Q000502:physiology; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000378:metabolism*
D008565:Membrane Proteins; D027701:Organic Cation Transport Proteins; C498687:Slc22a21 protein, mouse; C492019:Slc22a5 protein, mouse; D000074058:Solute Carrier Family 22 Member 5; D001622:Betaine; C002889:gamma-butyrobetaine; D000108:Acetylcarnitine; D011738:Pyrilamine; D002331:Carnitine