title: Development of a multiplex PCR assay for identification of Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis.
abstract: A multiplex PCR assay has been developed for the identification of the six common Campylobacter taxa associated with human gastroenteritis and/or septicaemia, namely Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis. The assay was developed using a combination of newly designed and published primers. It provided a specific PCR product for each of the five Campylobacter species and the one subspecies, and each of the PCR products was sufficiently distinguished by a difference in size by agarose gel electrophoresis. On evaluation of efficacy with 142 Campylobacter strains, the assay correctly identified all strains as 1 of the 6 Campylobacter taxa. This multiplex PCR assay is a rapid, simple and practical tool for identification of the six Campylobacter taxa commonly associated with gastroenteritis and/or septicaemia in humans, and offers an effective alternative to conventional biochemical-based assays.
authors.affiliation: Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Yamazaki-Matsune
authors.affiliation: Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka, Japan.
authors.forename: Masumi
authors.initials: M
authors.lastname: Taguchi
authors.affiliation: Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Seto
authors.affiliation: Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Kawahara
authors.affiliation: Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kawatsu
authors.affiliation: Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kumeda
authors.affiliation: Yodogawa Christian Hospital, Osaka, Japan.
authors.forename: Miyoshi
authors.initials: M
authors.lastname: Kitazato
authors.affiliation: Microbiological Department, Kobe Institute of Health, Hyogo, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Nukina
authors.affiliation: Department of Veterinary Public Health, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
authors.forename: Naoaki
authors.initials: N
authors.lastname: Misawa
authors.affiliation: Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka, Japan.
authors.forename: Teizo
authors.initials: T
authors.lastname: Tsukamoto
mesh_terms: D002167:Campylobacter / Q000145:classification* / Q000235:genetics; D002169:Campylobacter Infections / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005759:Gastroenteritis / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000382:microbiology; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S