title: Cryopreservation of Picea sitchensis (sitka spruce) embryogenic suspensor masses.
abstract: A cryopreservation method comprising sorbitol pre-growth treatment, DMSO cryoprotection and two-step controlled rate cooling has been optimized for differentiated embryogenic suspensor masses (ESM) of Picea sitchensis. The protocol was applied to clonal cultures from five different half-sibling families each represented by five different genotypes and their responses to cryopreservation assessed over 3 years. Nineteen of the 25 clonal lines tested survived LN and were capable of regrowth and producing stage 2-4 somatic embryos. Following the second subculture cycle of esm after they had been retrieved from cryogenic storage, post-cryopreservation regrowth was comparable with that of controls. A general linear model, multifactorial main effects plot revealed no significant differences between genotypes (p > 0.05) in response to cryopreservation, whereas significant differences between families (p < 0.05) were detected.
authors.affiliation: Plant Conservation Group, University of Abertay Dundee, Dundee, UK.
authors.forename: Samantha
authors.initials: S
authors.lastname: Gale
authors.forename: Allan
authors.initials: A
authors.lastname: John
authors.forename: Erica E
authors.initials: EE
authors.lastname: Benson
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D047108:Embryonic Development / Q000502:physiology; D016468:Forestry / Q000379:methods*; D005838:Genotype; D016014:Linear Models; D028222:Picea / Q000187:drug effects / Q000196:embryology* / Q000235:genetics; D012639:Seeds / Q000187:drug effects / Q000502:physiology*
chemical_list: D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide