An overview of epigenetic assays.
A significant portion of ongoing epigenetic research involves the investigation of DNA methylation and chromatin modification patterns seen throughout many biological processes. Over the last few years, epigenetic research has undergone a gradual shift and recent studies have been directed toward a genome-wide assessment. DNA methylation and chromatin modifications are essential components of the regulation of gene activity. DNA methylation effectively down-regulates gene activity by addition of a methyl group to the five-carbon of a cytosine base. Less specifically, modification of the chromatin structure can be carried out by multiple mechanisms leading to either the upregulation or down-regulation of the associated gene. Of the many assays used to assess the effects of epigenetic modifications, chromatin immunoprecipitation (ChIP), which serves to monitor changes in chromatin structure, and bisulfite modification, which tracks changes in DNA methylation, are the two most commonly used techniques.
DeAngelis
J Tyson
JT
Department of Biology, University of Alabama at Birmingham, Birmingham, AL 35294-1170, USA.
Farrington
Woodrow J
WJ
Tollefsbol
Trygve O
TO
D000818:Animals; D042002:Chromatin Assembly and Disassembly / Q000235:genetics; D047369:Chromatin Immunoprecipitation; D019175:DNA Methylation; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D044127:Epigenesis, Genetic / Q000235:genetics*; D005821:Genetic Techniques; D006801:Humans
D004798:Enzymes