[Experimental study on lyophilization of platelets].
The aim of this study was to search a procedure of platelet lyophilization and find a way of long-term storage of human platelets at normal temperature with smaller size and lighter weight, to be convenient to transport at long distance thus to meet the demands in accidents and war time. Human platelets were pretreated by freezing, the first and the second desiccation, and were added with reversible activation-inhibitors of platelets, DMSO and trehalose, then were rehydrated. At the same time, the recovery rate of platelets, platelet maximal aggregation induced by thrombin, coagulation of platelets, CD62p expression and PAC-1 expression were assayed. The results indicated that the recovery rate of the platelets was 56.29%. The platelet maximal aggregation induced by thrombin had no significant difference between lyophilized platelets and the fresh platelet-rich plasma (FPRP), but the aggregation of platelets induced by ADP or propyl gallate was decreased by 49.34% and 26.25%. Coagulation of the lyophilized platelets was not significantly different from FPRP. CD62p expression of the lyophilized platelets (42.36%) was higher than that in FPRP while PAC-1 expression was 2.12%. CD62p re-expression rate induced by thrombin was 50.88% and PAC-1 re-expression was 54.55%. It is concluded that the ability of recovered lyophilized platelets added with reversible activation-inhibitors, DMSO and trehalose to aggregate and coagulate has showed no significant difference as compared with FPRP. The reversible activation-inhibitors can decrease CD62p expression of lyophilized platelets, and may enhance their survival ability and prolongate survival time. Therefore the efficiency of lyophilizing platelets can be improved based on this freeze-drying procedure.
Liu
Jing-Han
JH
Department of Blood Transfusion, General Hospital of Chinses PLA, Beijing 100853, China. jinghan_liu@301hospital.cm.cn
Zhou
Jun
J
Wang
Dong-Mei
DM
Ouyang
Xi-Lin
XL
Xing
Yan-Chao
YC
Lu
Fa-Qiang
FQ
D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival; D005612:Freeze Drying / Q000379:methods*; D006801:Humans; D014199:Trehalose / Q000494:pharmacology
D014199:Trehalose