title: Enhancement of serological immune responses to foot-and-mouth disease vaccine by a supplement made of extract of cochinchina momordica seeds.
abstract: Foot-and-mouth disease (FMD) is a highly contagious disease affecting cloven-hoofed animals. Vaccination against FMD is a routine practice in many countries where the disease is endemic. This study was designed first to investigate the extract of the seeds of Momordica cochinchinensis (Lour.) Spreng. (ECMS) for its adjuvant effect on vaccination of inactivated FMDV antigens in a guinea pig model and then to evaluate the supplement of ECMS in oil-emulsified FMD vaccines for its immunopotentiation in pigs. The results indicated that ECMS and oil emulsion act synergistically as adjuvants to promote the production of FMDV- and VP1-specific immunoglobulin G (IgG) and subclasses in guinea pigs. A supplement of ECMS in a commercial FMD vaccine significantly enhanced FMDV-specific indirect hemagglutination assay titers as well as VP1-specific IgG and subclasses in pigs. Therefore, ECMS could be an alternative approach to improving swine FMD vaccination when the vaccine is poor to induce an effective immune response.
authors.affiliation: Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China.
authors.forename: Chenwen
authors.initials: C
authors.lastname: Xiao
authors.forename: Zahid Iqbal
authors.initials: ZI
authors.lastname: Rajput
authors.forename: Diwen
authors.initials: D
authors.lastname: Liu
authors.forename: Songhua
authors.initials: S
authors.lastname: Hu
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D006168:Guinea Pigs; D006386:Hemagglutination Tests / Q000662:veterinary; D007273:Injections, Intramuscular; D019684:Magnoliopsida / Q000737:chemistry*; D011897:Random Allocation; D012639:Seeds / Q000737:chemistry; D013223:Statistics as Topic; D034421:Sus scrofa; D016896:Treatment Outcome; D014611:Vaccination / Q000379:methods / Q000662:veterinary; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D004365:Drugs, Chinese Herbal; D014765:Viral Vaccines