[Evaluation of the transcription of locus rfb for the biosynthesis of LPS in Leptospira interrogans subtype hardjoprajitno].
Plasmid pGL4W15-96 was constructed from the pGL4W74 vector without promoter for kanamycin and a sequence of 480pb containing the supposed J15 promoter with the objective of confirming the role of J15 sequence as a promoter for Leptospira, which restored the transcription of the gene of resistance to kanamycin in Escherichia coli and Leptospira biflexa, corroborating this way the function of the insertion as a promoter for both organisms.
Casas
Erika Margarita Carrillo
EM
Facultad de Medicina Veterinaria y Zootecnia, UNAM, México.
Ríos
Raquel Rojano
RR
Bulach
Dieter
D
Adler
Ben
B
de la Peña-Moctezuma
Alejandro
A
D001426:Bacterial Proteins; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects; D007612:Kanamycin / Q000494:pharmacology*; D007921:Leptospira interrogans / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D019081:O Antigens; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D019081:O Antigens; C079397:rfb protein, Bacteria; D007612:Kanamycin