Body fluid proteomics for biomarker discovery: lessons from the past hold the key to success in the future.
Sparked by the article from Lescuyer and colleagues in a recent issue, we aim here to further encourage interest in and discussion of clinically relevant biomarker research. We express our view on proteomics for biomarker discovery by addressing multiple relevant issues, including the inherent differences between biological fluids (and how these differences affect current analytical approaches) and experimental design to maximize the efficiency of moving from the bench to the bedside. Herein, we also include suggestions for definition of the term "biomarker", based on the use of a set of universal characterization/validation requirements, and illustrate several recent examples of successful transitions of benchtop proteomic studies work to clinical practice.
Good
David M
DM
Department of Chemistry, University of Wisconsin-- Madison, Madison, Wisconsin 53706, USA.
Thongboonkerd
Visith
V
Novak
Jan
J
Bascands
Jean-Loup
JL
Schanstra
Joost P
JP
Coon
Joshua J
JJ
Dominiczak
Anna
A
Mischak
Harald
H
D000818:Animals; D015415:Biomarkers / Q000737:chemistry* / Q000378:metabolism*; D001826:Body Fluids / Q000737:chemistry* / Q000378:metabolism*; D005544:Forecasting; D006801:Humans; D020543:Proteome / Q000737:chemistry* / Q000378:metabolism*; D040901:Proteomics / Q000639:trends*; D015203:Reproducibility of Results
D015415:Biomarkers; D020543:Proteome