title: Identification and characterization of two novel human papillomaviruses (HPVs) by overlapping PCR: HPV102 and HPV106.
abstract: Complete genomes of HPV102 (8,078 bp) and HPV106 (8,035 bp) were PCR amplified and cloned from cervicovaginal cells of a 49-year-old Hispanic female with reactive changes on her Pap test and a 42-year-old Hispanic female with a Pap test diagnosis of atypical squamous cells of unknown significance (ASCUS), respectively. The nucleotide sequence similarity of the complete L1 open reading frame (ORF) determined that HPV102 and HPV106 are most closely related to HPV83 (84.1 % identity) and HPV90 (83.5 % identity), respectively, placing them in the genital HPV groups, papillomaviruses species alpha3 and alpha15. HPV102 and HPV106 contain five early genes (E6, E7, E1, E2, and E4) and two late genes (L2 and L1), and both lack an E5 ORF. On the basis of phylogenetic analyses and available clinical information, these two novel HPV types expand the heterogeneity of HPVs detected in the lower genital tract.
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY, USA.
authors.forename: Zigui
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Schiffman
authors.affiliation: Proyecto Epidemiológico Guanacaste, Costa Rican Foundation for Health Sciences, San José, Costa Rica.
authors.forename: Rolando
authors.initials: R
authors.lastname: Herrero
authors.affiliation: Department of Pediatrics, Department of Epidemiology and Population Health, Department of Obstetrics, Gynecology and Woman's Health and Albert Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, NY, USA.|Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY, USA.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Burk
mesh_terms: D052685:Alphapapillomavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D052776:Female Urogenital Diseases / Q000821:virology*; D005814:Genes, Viral; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D030361:Papillomavirus Infections / Q000821:virology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins