Differentiation of Vitis vinifera varieties by MALDI-MS analysis of the grape seed proteins.
Until now the study of pathogenic related proteins in grape juice and wine, performed by ESI-MS, LC/ESI-MS, and MALDI/MS, has been proposed for differentiation of varieties. In fact, chitinases and thaumatin-like proteins persist through the vinification process and cause hazes and sediments in bottled wines. An additional instrument, potentially suitable for the grape varieties differentiation, has been developed by MALDI/MS for the grape seed protein analysis. The hydrosoluble protein profiles of seeds extract from three different Vitis vinifera grape (red and white) varieties were analyzed and compared. In order to evaluate the environmental conditions and harvest effects, the seed protein profiles of one grape variety from different locations and harvests were studied.
Pesavento
Ivana Chiara
IC
CNR-ISTM, Sezione di Padova, Corso Stati Uniti 4, 35100 Padova, Italy.
Bertazzo
Antonella
A
Flamini
Riccardo
R
Vedova
Antonio Dalla
AD
De Rosso
Mirko
M
Seraglia
Roberta
R
Traldi
Pietro
P
D014644:Genetic Variation; D010936:Plant Extracts / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis*; D012639:Seeds / Q000737:chemistry*; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D027843:Vitis / Q000737:chemistry* / Q000145:classification
D010936:Plant Extracts; D010940:Plant Proteins