Effects of date seed oil on normal human skin in vitro.
Oxidative stress has been implicated in various skin diseases through the generation of reactive oxygen species and the depletion of endogenous antioxidant systems. The administration of antioxidants or free radical scavengers is reportedly helpful, notably in order to enhance the healing process. We investigated the protective effect of one new natural product:"date seed oil: DSO" against hydrogen peroxide (H(2)O(2))-induced oxidative stress, in terms of lipid peroxidation, depletion of endogenous antioxidant defense enzymes such as glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), one day after a 2 h exposure to H(2)O(2), using human skin organ culture as an in vitro model. In the investigated model system, DSO has significant protective effect, by inhibition of damage caused by H(2)O(2), endowing a radical scavenging ability. Treatment of skin with DSO inhibited H(2)O(2)-induced lipid peroxidation. In addition, this oil inhibited H(2)O(2)-induced depletion of antioxidant defense components, such as superoxide dismutase and catalase. We conclude that DSO could be useful in the attenuation of H(2)O(2)-induced oxidative stress-mediated skin diseases in human skin, possibly due to antioxidant properties.
Dammak
Ines
I
Unité de Recherche, Pathologies Humaines et Stress Oxydatif, Institut Supérieur de Biotechnologie de Sfax, 3038, Sfax, Tunisia. inesdammak@yahoo.fr
Ben Abdallah
Fatma
F
Boudaya
Sonia
S
Keskes
Laila
L
Besbes
Souhail
S
El Gaied
Amel
A
Attia
Hamadi
H
Turki
Hamida
H
Hentati
Basma
B
D000704:Analysis of Variance; D000975:Antioxidants / Q000494:pharmacology*; D028023:Arecaceae; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008315:Malondialdehyde / Q000378:metabolism; D009924:Organ Culture Techniques; D010938:Plant Oils / Q000494:pharmacology*; D012639:Seeds; D012867:Skin / Q000187:drug effects* / Q000201:enzymology; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D010938:Plant Oils; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase