title: Age-related differences in sensitivity of peripheral blood monocytes to lipopolysaccharide and Staphylococcus aureus toxin B in atopic dermatitis.
abstract: As shown by atopy patch tests, atopic dermatitis (AD) is dominated in its acute phase by the development of a specific T(H)2 response after exposure of the skin to common environmental antigens. Relying on our previous data showing that Staphylococcus aureus enterotoxin B (SEB) induced the activation of monocyte-derived dendritic cells (DCs) through Toll-like receptor (TLR)2 and that SEB-pulsed DCs commit allogenic naive T cells into T(H)2, we assessed monocytes sensitivity to SEB and lipopolysaccharide (LPS) in a group of children and adult patients with AD. Monocytes from AD patients (15 adults with mostly severe disease and 15 children with mild to moderate disease) exhibited an activated and tolerant state as supported by (i) secretion of large amounts of IL-6, IL-10, and tumor necrosis factor-alpha even in the absence of stimulation; (ii) their inability to modulate neither HLA-DR and CD54 nor TLR2 and TLR4 expression after in vitro challenge with SEB; (iii) inhibition of IL-12p70 secretion in response to LPS. Interestingly, monocytes from some of the children studied responded to in vitro challenge with LPS, suggesting new hypotheses to explain disease regression. Our data support the notion that monitoring sensitivity of monocytes to bacterial toxins could prove useful to assess disease progression and prognosis in AD.
authors.affiliation: INSERM, U563, Centre de Physiopathologie de Toulouse Purpan, Toulouse, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Mandron
authors.forename: Marie-Françoise
authors.initials: MF
authors.lastname: Ariès
authors.forename: Franck
authors.initials: F
authors.lastname: Boralevi
authors.forename: Hélène
authors.initials: H
authors.lastname: Martin
authors.forename: Marie
authors.initials: M
authors.lastname: Charveron
authors.forename: Alain
authors.initials: A
authors.lastname: Taieb
authors.forename: Christian
authors.initials: C
authors.lastname: Davrinche
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000942:Antigens, Bacterial / Q000276:immunology* / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000378:metabolism; D003876:Dermatitis, Atopic / Q000276:immunology*; D018450:Disease Progression; D004768:Enterotoxins / Q000276:immunology* / Q000494:pharmacology; D005260:Female; D006801:Humans; D007223:Infant; D008070:Lipopolysaccharides / Q000276:immunology* / Q000494:pharmacology; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D011237:Predictive Value of Tests; D011379:Prognosis; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D051195:Toll-Like Receptor 2 / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D016207:Cytokines; D004768:Enterotoxins; D008070:Lipopolysaccharides; C495342:TLR2 protein, human; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; C031818:enterotoxin B, staphylococcal