A model selection approach for robust spatio-temporal analysis of dynamics in 4D fluorescence videomicroscopy.
We describe a novel automatic approach for vesicle trafficking analysis in 3D+T videomicroscopy. Tracking individually objects in time in 3D+T videomicroscopy is known to be a very tedious job and leads generally to unreliable results. So instead, our method proceeds by first identifying trafficking regions in the 3D volume and next analysing at them the vesicle trafficking. The latter is viewed as significant change in the fluorescence of a region in the image. We embed the problem in a model selection framework and we resolve it using dynamic programming. We applied the proposed approach to analyse the vesicle dynamics related to the trafficking of the RAB6A protein between the Golgi apparatus and ER cell compartments.
Bechar
Ikhlef
I
University Rene Descartes (Paris 5), Paris, France. ikhlef.bechar@jouy.inra.fr
Trubuil
Alain
A
D000465:Algorithms; D001331:Automation; D003196:Computer Graphics; D004721:Endoplasmic Reticulum / Q000473:pathology; D004867:Equipment Design; D006056:Golgi Apparatus / Q000473:pathology; D006801:Humans; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D018715:Microscopy, Video / Q000295:instrumentation* / Q000379:methods*; D015233:Models, Statistical; D008962:Models, Theoretical; D010363:Pattern Recognition, Automated; D012815:Signal Processing, Computer-Assisted; D020691:rab GTP-Binding Proteins / Q000378:metabolism
C094135:Rab6 protein; D020691:rab GTP-Binding Proteins