Electroporation-induced cell lysis in SWLA-2 hybridomas.
This paper describes experimental results involving the percentage cell lysis in SWLA-2 murine hybridomas produced by square wave electric field pulses of 100, 200, and 300 V across a 1 mm gap width in a standard cuvette. Pulse lengths were of 0.2 and 0.6 ms duration; 1, 2, or 3 pulses were applied with 100 ms time interval between pulses. Cells were cultured and separate samples examined at 48 hours to determine cell mortality. Nearly 90% cell mortality was produced by applying 3 pulses at of 0.6 ms duration at 300 V.
Oakley
Barbara A
BA
Sch. of Eng. & Comput. Sci., Oakland Univ., Rochester, MI 48309, USA. oakley@oakland.edu
Gross
Brooks A
BA
Martens
Sara G
SG
Hanna
Darrin M
DM
Stryker
Gabrielle A
GA
D000818:Animals; D001698:Biomedical Engineering; D002470:Cell Survival; D004574:Electromagnetic Fields / Q000009:adverse effects; D018274:Electroporation / Q000379:methods*; D006825:Hybridomas / Q000166:cytology*; D051379:Mice