[Study on the expression of suppressor of cytokine signaling-1 in liver and spleen of septic mice].
OBJECTIVE
To investigate the change in suppressor of cytokine signaling-1 (SOCS1) gene in the liver and the spleen of septic mouse, and to find out its probable mechanisms of action in sepsis.


METHODS
Cecal ligation and puncture (CLP) was adopted to reproduce sepsis model. The liver and the spleen tissues were harvested and RNA and protein were respectively extracted. The contents of the regulatory genes SOCS1 mRNA were determined by reverse transcription-polymerase chain reaction (RT-PCR) and regulatory content of protein was detected by Western blotting. The SPSS statistics software was adopted to calculate the correlation.


RESULTS
The expressions of SOCS1 on gene and protein in the liver were markedly upregulated at 6 th hour. The gene expression peaked at the 24 th hour (P<0.05).The expression of protein was persistently high. However, the expression of SOCS1 was only detected in the spleen, and it obviously rose in strength with the passage of time, and it remained in a high level. By statistical analysis, positive correlations were found between the gene and protein expressions of SOCS1 (y=0.110+/-5.765 x 10(-3) x, r=0.837, F=93.309, P<0.01).


CONCLUSIONS
CLP induced sepsis can induce the up-regulation of the expressions of SOCS1, indicating that SOCS1 play important role in the change in immune system in sepsis. They may be used to intervene sepsis so as to improve the outcome of sepsis.
Zhao
Feng
F
Department of Emergency, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong, China.
Pan
Wen
W
Liang
Yan-bing
YB
Tang
Hao
H
Chen
Zhi-bin
ZB
Lin
Miao-xia
MX
Yang
Xiao-yan
XY
Zhou
Wei-xiong
WX
Ma
Zhong-fu
ZF
D000818:Animals; D004195:Disease Models, Animal; D015870:Gene Expression; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D018805:Sepsis / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism*; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; C491590:Socs1 protein, mouse; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins