title: [Optimization of manninotriose preparation from stachoyse].
abstract: OBJECTIVE
  To optimize the processing of mannatriose preparation from stachyose by acidolysis.
  
  
  METHODS
  An orthogonal experiment was performed to select a better condition of acidolysis. Fructoes and stachyose was determined by HPLC.
  
  
  RESULTS
  Among the factors set in the present work, temperature and time have little influence on the acidolysis, while the effect of pH value was significant (P < 0.05). The best processing was selected as heating stachosy solution for 12h at pH 2.5 and 90 degrees C.
  
  
  CONCLUSIONS
  It is practicable to prepare manninotriose from stachyoses by acidolysis.
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China. weihong204@163.com
authors.forename: Wei-hong
authors.initials: WH
authors.lastname: Wu
authors.forename: Xue-sen
authors.initials: XS
authors.lastname: Wen
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000143:Acids / Q000378:metabolism; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009844:Oligosaccharides / Q000737:chemistry*; D032267:Rehmannia / Q000737:chemistry*; D012996:Solutions; D013696:Temperature; D013997:Time Factors; D014312:Trisaccharides / Q000302:isolation & purification*
chemical_list: D000143:Acids; D009844:Oligosaccharides; D012996:Solutions; D014312:Trisaccharides; C069155:manninotriose; C005695:stachyose