title: Inhibition of HCV replicon cell growth by 2-arylbenzofuran derivatives isolated from Mori Cortex Radicis.
abstract: Medicinal herbs are increasingly used in the search for safe and efficient drug candidates for hepatitis C virus infection. In this study, we have investigated the anti-HCV effect of compounds from Mori Cortex Radicis. During a screening for extracts with anti-HCV affinity from medicinal plants (173 species), the methanol extract of Mori Cortex Radicis was selected. Fractionation of the extract by monitoring antiviral activity with a replicon cell-based assay resulted in the isolation of five compounds, mulberroside C ( 1), moracin P ( 2), moracin O ( 3), moracin M ( 4) and mulberrofuran K ( 5) from the ethyl acetate-soluble fraction. Compounds 1 approximately 4 showed significant inhibitory activities. Compounds 2 and 3 showed potent inhibitory activity (IC (50) 35.6 microM, 80.8 microM, respectively) in the replicon cell assay, which was confirmed by NS3 helicase inhibitory activity (IC (50) 42.9 microM, 27.0 microM, respectively).
authors.affiliation: R&D Center, Dong-Wha Pharmaceutical Ind. Co. Ltd., Anyang, Korea.
authors.forename: Hyun Yong
authors.initials: HY
authors.lastname: Lee
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Yum
authors.forename: Yang Kook
authors.initials: YK
authors.lastname: Rho
authors.forename: Seong Jun
authors.initials: SJ
authors.lastname: Oh
authors.forename: Hyun Sik
authors.initials: HS
authors.lastname: Choi
authors.forename: Hwan Bong
authors.initials: HB
authors.lastname: Chang
authors.forename: Dong Hwa
authors.initials: DH
authors.lastname: Choi
authors.forename: Moon-Jeong
authors.initials: MJ
authors.lastname: Leem
authors.forename: Eun Joo
authors.initials: EJ
authors.lastname: Choi
authors.forename: Jei Man
authors.initials: JM
authors.lastname: Ryu
authors.forename: Soon Bong
authors.initials: SB
authors.lastname: Hwang
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D016174:Hepacivirus / Q000187:drug effects*; D006801:Humans; D015394:Molecular Structure; D029586:Moraceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts