title: Haeamtang induces apoptosis of colon cancer HT-29 cells through activation of caspase-3.
abstract: The effect of Haeamtang (HAT) on the colon cancer HT-29 cells was investigated in this study. A water extract of HAT significantly decreased the number of HT-29 cells in a dose-and time-dependent manner as determined by a MTT assay. Flow cytometry results revealed a dose- and time-dependent increase of dead cells in HT-29 cells treated with HAT extract. The anticancer activity of the H AT extract is attributed to apoptosis induced in HT-29 cells, which was demonstrated by increased caspase-3 activity and poly-ADP-ribose polymerase fragmentation. A selective caspase inhibitor, z-VAD-fmk, inhibited the HAT-induced cell death. Taken together, these results demonstrate that HAT extract induces apoptosis in HT-29 cells.
authors.affiliation: Cancer Preventive Material Development Research Center, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul, 130-701, Republic of Korea.
authors.forename: Phil-Dong
authors.initials: PD
authors.lastname: Moon
authors.forename: Hyun-Na
authors.initials: HN
authors.lastname: Koo
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Ho-Jeong
authors.initials: HJ
authors.lastname: Na
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Gab-Soo
authors.initials: GS
authors.lastname: Hwang
authors.forename: Na-Ra
authors.initials: NR
authors.lastname: Han
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Um
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism*; D004789:Enzyme Activation; D005434:Flow Cytometry; D019073:HT29 Cells; D006801:Humans; D008519:Medicine, Traditional; D010936:Plant Extracts
chemical_list: C526215:Haeamtang; D010936:Plant Extracts; D053148:Caspase 3