Preparation and application of L-cysteine-modified CdSe/CdS core/shell nanocrystals as a novel fluorescence probe for detection of nucleic acid.
The water-soluble L-cysteine-modified CdSe/CdS core/shell nanocrystals (expressed as CdSe/CdS/Cys nanocrystals) have been synthesized in aqueous by using L-cysteine as stabilizer. The size, shape, component and spectral property of CdSe/CdS/Cys nanocrystals were characterized by high-resolution transmission electron microscope (HRTEM), energy dispersive X-ray fluorescence (EDX), infrared spectrum (IR) and photoluminescence (PL). The results showed that the spherical CdSe/CdS/Cys nanocrystals with an average diameter of 2.3 nm have favorable fluorescent property, theirs photostability and fluorescence intensity are enhanced greatly after overcoating with CdS. The cysteine modified on the surface of core/shell CdSe/CdS nanocrystals renders the nanocrystals water-soluble and biocompatible. Based on the fluorescence quenching of the nanocrystals in the presence of calf thymus deoxyribonucleic acid (ct-DNA), a fluorescence quenching method has been developed for the determination of ct-DNA by using the nanocrystals as a novel fluorescence probe. The pH value of the system was selected at pH 7.4, with excitation and emission wavelength at 380 and 522 nm, respectively. Under the optimal conditions, the fluorescence quenching intensity of the system is linear with the concentration of ct-DNA in the range of 0.1-3.5 microg/mL (r=0.9987). The detection limit is 0.06 microg/mL. And two synthetic samples were analyzed satisfactorily.
Huang
Fenghua
F
The Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, PR China.
Chen
Guonan
G
D000818:Animals; D019187:Cadmium Compounds / Q000138:chemical synthesis*; D002417:Cattle; D003545:Cysteine / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D015335:Molecular Probes / Q000138:chemical synthesis*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009696:Nucleic Acids / Q000032:analysis*; D009994:Osmolar Concentration; D018036:Selenium Compounds / Q000138:chemical synthesis*; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D013440:Sulfides / Q000138:chemical synthesis*
D019187:Cadmium Compounds; D015335:Molecular Probes; D009696:Nucleic Acids; D018036:Selenium Compounds; D013440:Sulfides; C034939:cadmium sulfide; D004247:DNA; C427203:calf thymus DNA; C058667:cadmium selenide; D003545:Cysteine