A new sensitive and selective method for analysis of ochratoxin A in grape and wine by direct liquid chromatography/surface-activated chemical ionization tandem mass spectrometry.
A new sensitive and selective analytical method for the analysis of ochratoxin A (OTA) in grape and wine was developed by coupling liquid chromatography and surface activated chemical ionization and mass spectrometry with multistage fragmentation (LC/SACI-MS(3)). A high flow gradient was used to strongly reduce the matrix effect phenomenon, and the wine sample was directly injected onto the chromatographic column without sample pre-concentration or purification steps. The amount of OTA was determined for two grape extracts and the amount of OTA, percent accuracy error and percent precision error were analyzed for 15 wine samples. An excellent limit of detection of 0.02 ng/mL was achieved, and the limit of quantification was at least 20-fold lower than the maximum legal limit for OTA (2 ppb). Due to the low limit of quantification, this novel method is a potential tool for official OTA screening purposes.
Flamini
Riccardo
R
CRA, Istituto Sperimentale per la Viticoltura, viale XXVIII aprile 26, 31015 Conegliano (TV), Italy. riccardo.flamini@entecra.it
Vedova
Antonio Dalla
AD
De Rosso
Mirko
M
Panighel
Annarita
A
D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis; D008832:Microchemistry / Q000379:methods; D009183:Mycotoxins / Q000032:analysis*; D036103:Nanotechnology; D009793:Ochratoxins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D027843:Vitis / Q000737:chemistry* / Q000382:microbiology; D014920:Wine / Q000032:analysis*
D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A