title: Cell trapping in activated micropores for functional analysis.
abstract: This paper presents a novel device which provides the opportunity to perform high-throughput biochemical assays on different individual cells. In particular, the proposed device is suited to screen the rare cells in biological samples for early stage cancer diagnosis and explore their biochemical functionality. In the process, single cells are precisely positioned and captured in activated micropores. To show the performance of the proposed device, cultured yeast cells and human epithelial circulating tumor cells are successfully captured.
authors.affiliation: Electr. Eng. Dept., Stanford Univ., CA 94305, USA. atalasaz@stanford.edu
authors.forename: AmirAli H
authors.initials: AH
authors.lastname: Talasaz
authors.forename: Ashley A
authors.initials: AA
authors.lastname: Powell
authors.forename: Patrik
authors.initials: P
authors.lastname: Stahl
authors.forename: Mostafa
authors.initials: M
authors.lastname: Ronaghi
authors.forename: Stefanie S
authors.initials: SS
authors.lastname: Jeffrey
authors.forename: Michael
authors.initials: M
authors.lastname: Mindrinos
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Davis
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002468:Cell Physiological Phenomena; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D016062:Porosity; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials