Ultrastructural characteristics of bone marrow in patients with hematological disease: a study of 13 cases.
There are few transmission electron microscopic studies on bone marrow biopsies of patients with hematological disease owing to the difficulty of overcoming the artifacts of decalcification. Following the fixation of bone marrow biopsies thoroughly before a mild decalcification procedure, ultrastructural studies were performed on 13 patients with varied hematological diseases. Notable features included blood cell disorganization, fibroblast activation, myofibroblast transformation, as well as accumulation of collagen and extracellular amorphous matrix. In addition, excessive blood cell death in leukemia, apoptosis, and macrophage phagocytosis in myelodysplastic syndrome and polycythemia vera, as well as degranulation of eosinophils and megakaryocytes in chronic idiopathic myelofibrosis were predominant, respectively. The observations suggest that polyclonal fibroblast proliferation and extracellular matrix accumulation may result from inflammation resulting from excessive cell death and active material release of blood cells in the bone marrow of patients with hematological disease.
Ru
Yong-xin
YX
Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.
Zhao
Shi-xuan
SX
Liu
En-bing
EB
Yang
Qin-ying
QY
Liu
Jin-hua
JH
Pang
Tian-xiang
TX
Chen
Hui-shu
HS
D000293:Adolescent; D000328:Adult; D001706:Biopsy; D001853:Bone Marrow / Q000648:ultrastructure*; D001854:Bone Marrow Cells / Q000648:ultrastructure*; D002648:Child; D002908:Chronic Disease; D022083:Cytoplasmic Structures / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000648:ultrastructure; D005260:Female; D005347:Fibroblasts / Q000648:ultrastructure; D006402:Hematologic Diseases / Q000235:genetics / Q000473:pathology*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000473:pathology; D007952:Leukemia, Plasma Cell / Q000235:genetics / Q000473:pathology; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics / Q000473:pathology; D011087:Polycythemia Vera / Q000235:genetics / Q000473:pathology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000473:pathology; D055728:Primary Myelofibrosis / Q000235:genetics / Q000473:pathology