title: [Inhibitory effects of anisodamine and pentoxifylline on the expression of lipopolysaccharide -induced intercellular adhesion molecule-1 in rat cardiac muscle].
abstract: OBJECTIVE
  To investigate the inhibitory effects of anisodamine (654-2) and pentoxifylline (PTX) on the expression of lipopolysaccharide (LPS)-induced intercellular adhesion molecule-1 (ICAM-1) in rat cardiac muscle in vivo.
  
  
  METHODS
  The animals were randomly divided into five groups (each n=6): (1) normal control group, (2) model group, (3) 654-2 treated group, (4) PTX treated group and (5) 654-2+PTX treated group. The endotoxemia model was reproduced by intravenous injection LPS 5 mg/kg. The expression of ICAM-1 protein in rat cardiac muscle was assayed by Western blotting at 0, 2, 4, 6, 8, 10 hours after intravenous LPS injection. Then the expression of ICAM-1 protein in different groups was assayed at different time points.
  
  
  RESULTS
  The changes in expression of ICAM-1 in rat cardiac muscle after LPS injection were in a time-dependent pattern, gradually elevating to approach the peak at 6 th, then it lowered, but it still appeared at 10 hours (P<0.05). Western blotting also showed that ICAM-1 protein with decreased with pre-treatment of 654-2 or PTX respectively (both P<0.01). It was reduced to a much lower level when the animals were pretreated with a combination of 654-2 and PTX, compared with the group of 654-2 alone or PTX alone (both P<0.05).
  
  
  CONCLUSIONS
  The combination of 654-2 and PTX may play a protective role in rat against injury to cardiac muscle induced by LPS in vivo via inhibiting the production of ICAM-1 protein.
authors.affiliation: Intensive Care Unit, the Second Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Sun
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Gu
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Ai-ling
authors.initials: AL
authors.lastname: Zhou
authors.forename: Ya-e
authors.initials: YE
authors.lastname: Hu
mesh_terms: D000818:Animals; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity; D009206:Myocardium / Q000378:metabolism*; D010431:Pentoxifylline / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012991:Solanaceous Alkaloids / Q000494:pharmacology*
chemical_list: D008070:Lipopolysaccharides; D012991:Solanaceous Alkaloids; C003922:anisodamine; D018799:Intercellular Adhesion Molecule-1; D010431:Pentoxifylline