The reference point method in primer design.
The conflicts between several design objectives for PCR primers are computationally resolved by specifying a set of ideal parameters and searching for primer pairs whose parameters approximate this ideal point as close as possible. It thus becomes feasible to identify an "optimum" and to efficiently compute it. User-specified target regions, primer conditions, and procedural conditions are obeyed.
Kämpke
Thomas
T
Forschungsinstitut fur anwendungsorientierte Wissensverarbeitung/n Ulm, Germany.
D017931:DNA Primers / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000592:standards; D012016:Reference Values; D017422:Sequence Analysis, DNA; D012984:Software
D017931:DNA Primers