[Effects of a high-carbohydrate, low-fat diet on the levels of serum phospholipids and triglycerides].
OBJECTIVE
To study the effects of a High-carbohydrate, Low-fat (HC/LF) diet on the levels of phospholipids and triglycerides in serum and analyze their association with anthropometric indexs and serum biochemical traits.


METHODS
56 healthy volunteers had a HC/LF diet for 6 days. The body weight, waist circumference, hip circumference, biceps circumference, and skinfold thickness were measured before and after the HC/LF diet. Venous blood was taken after fasting of 12 hours. The levers of high density lipoprotein-phospholipid (HDL-PL), high density lipoprotein-triglyceride (HDL-TG), high density lipoprotein-cholesterol (HDL-C) were enzymatically determined after precipitation of serum apoB containing lipoproteins with phosphotungistic acid and non-LDL-phospholipid (NLDL-PL), non-LDL-triglyceride (NLDL-TG), non-LDL-Cholesterol (NLDL-C) levels were enzymatically determined after precipitation of serum LDL with polyethylene sulfate. The levels of serum LDL-PL, LDL-TG, and LDL-C were calculated by minus of non-LDL from serum PL, TG, and TC levels, respectively. The level of TRL-TG was calculated by minus of HDL-TG, LDL-TG from total TG. The level of serum insulin was measured by electrochemical luminescence.


RESULTS
The levels of fasting serum PL, HDL-PL and LDL-PL decreased significantly after HC/LF diet, whereas the levels of TG, HDL-TG and insulin increased. Correlation analyses indicated that after HC/LF diet the change of serum PL (APL) had a negative correlation with the change of BMI(deltaBMI),a positive correlation with the change of HDL-TG(deltaHDL-TG) and insulin (delta insulin). The change of serum LDL-PL (deltaLDL-PL) had a negative correlation with deltaBMI and waist circumference (deltaAWC), meanwhile the change of HDL-PL (deltaHDL-PL) had a negative correlation with deltaHDL-TG, a positive correlation with the change of HDL-C (deltaHDL-L). A positive correlation existed between deltaHDL-TG and wais/hip circumference ratio (deltaW/H).


CONCLUSIONS
HC/LF diet can decrease serum PL, HDL-PL level but increase serum levels of TG and insulin. The change of serum PL level had a negative correlation with deltaBMI,a positive correlation with deltaHDL-TG and delta insulin.
Tang
Hui
H
Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Li
Zheng-ke
ZK
Lin
Jia
J
Huang
Xin
X
Gan
Chan-fen
CF
Li
Rong-hui
RH
Hu
Wen
W
Fang
Ding-zhi
DZ
D000328:Adult; D000886:Anthropometry; D018752:Diet, Fat-Restricted; D004040:Dietary Carbohydrates; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood; D008075:Lipoproteins, HDL / Q000097:blood; D008297:Male; D010743:Phospholipids / Q000097:blood*; D014280:Triglycerides / Q000097:blood*; D055815:Young Adult
D004040:Dietary Carbohydrates; D007328:Insulin; D008075:Lipoproteins, HDL; D010743:Phospholipids; D014280:Triglycerides