title: Cyclic changes in metabolic state during the life of a yeast cell.
abstract: Budding yeast undergo robust oscillations in oxygen consumption during continuous growth in a nutrient-limited environment. Using liquid chromatography-mass spectrometry and comprehensive 2D gas chromatography-mass spectrometry-based metabolite profiling methods, we have determined that the intracellular concentrations of many metabolites change periodically as a function of these metabolic cycles. These results reveal the logic of cellular metabolism during different phases of the life of a yeast cell. They may further indicate that oscillation in the abundance of key metabolites might help control the temporal regulation of cellular processes and the establishment of a cycle. Such oscillations in metabolic state might occur during the course of other biological cycles.
authors.affiliation: Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9038, USA. benjamin.tu@utsouthwestern.edu
authors.forename: Benjamin P
authors.initials: BP
authors.lastname: Tu
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Mohler
authors.forename: Jessica C
authors.initials: JC
authors.lastname: Liu
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Dombek
authors.forename: Elton T
authors.initials: ET
authors.lastname: Young
authors.forename: Robert E
authors.initials: RE
authors.lastname: Synovec
authors.forename: Steven L
authors.initials: SL
authors.lastname: McKnight
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006418:Heme / Q000096:biosynthesis; D009249:NADP / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism; D013997:Time Factors
chemical_list: D006418:Heme; D009249:NADP; D013455:Sulfur; D000105:Acetyl Coenzyme A