title: Surface re-engineering of pancreatic islets with recombinant azido-thrombomodulin.
abstract: Chemoselective conjugation of an azido-functionalized thrombomodulin to pancreatic islets was achieved by Staudinger ligation to a surface-bound bifunctional poly(ethylene glycol) linker. The presence of the tethered thrombomodulin resulted in a significant increase in the production of activated protein C with a reduction in islet-mediated thrombogenicity. This report highlights the potential of tissue-targeted chemistry to reduce donor cell mediated procoagulant and proinflammatory responses.
authors.affiliation: Department of Surgery, Emory University, Atlanta, GA 30322, USA.
authors.forename: Cherie L
authors.initials: CL
authors.lastname: Stabler
authors.forename: Xue-Long
authors.initials: XL
authors.lastname: Sun
authors.forename: Wanxing
authors.initials: W
authors.lastname: Cui
authors.forename: John T
authors.initials: JT
authors.lastname: Wilson
authors.forename: Carolyn A
authors.initials: CA
authors.lastname: Haller
authors.forename: Elliot L
authors.initials: EL
authors.lastname: Chaikof
mesh_terms: D000818:Animals; D001386:Azides / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D007515:Islets of Langerhans / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry; D013499:Surface Properties; D018180:Thrombomodulin / Q000737:chemistry*
chemical_list: D001386:Azides; D003432:Cross-Linking Reagents; D018180:Thrombomodulin; D011092:Polyethylene Glycols