The antimicrobial effect of biopure MTAD on eight strains of Enterococcus faecalis: an in vitro investigation.
The purpose of this investigation was to determine the antimicrobial effect of MTAD as a final irrigant on eight strains of Enterococcus faecalis (E. faecalis) and to measure the minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) of MTAD. The roots of 240 extracted human teeth were instrumented using 1.3% NaOCl and 17% EDTA. The roots were divided into eight groups and contaminated with one of eight strains of E. faecalis. After irrigating with 1.3% NaOCl, the root canal and the external surfaces were exposed to MTAD for 5 minutes. Roots or dentin shavings were cultured to determine the growth of E. faecalis. The results showed that this treatment regimen was effective in completely eliminating growth in seven of eight strains of E. faecalis. The MIC/MLC tests showed that MTAD inhibited most strains of E. faecalis growth when diluted 1:8192 times and killed most strains of E. faecalis when diluted 1:512 times.
Newberry
Bradley M
BM
School of Dentistry, Loma Linda University, Loma Linda, California 92350, USA.
Shabahang
Shahrokh
S
Johnson
Neal
N
Aprecio
Raydolfo M
RM
Torabinejad
Mahmoud
M
D019343:Citric Acid / Q000494:pharmacology*; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004305:Dose-Response Relationship, Drug; D004318:Doxycycline / Q000494:pharmacology*; D013293:Enterococcus faecalis / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D011136:Polysorbates / Q000494:pharmacology*; D012388:Root Canal Irrigants / Q000494:pharmacology*
C475043:MTAD (intracanal irrigant); D011136:Polysorbates; D012388:Root Canal Irrigants; D019343:Citric Acid; D004318:Doxycycline