[Expression and action of keratinocyte growth factor, keratinocyte growth factor receptor on CaSki cell].
OBJECTIVE
To investigate the expressions of keratinocyte growth factor (KGF), keratinocyte growth factor receptor (KGFR) in CaSki cell and action of keratinocyte growth factor on proliferation, migration of the CaSki cell.


METHODS
ELISA and Western blot methods were used to determine the protein expressions of the KGF and KGFR in CaSki cell respectively: 3H-Thymidine incorporation method was used to determine the effect of recombinant human KGF and anti-KGF on CaSki cell proliferation; Millicell-PCF was used to determine the effect of recombinant human KGF, anti-KGF on CaSki cell migration.


RESULTS
Both KGF and KGFR were expressing in the CaSki cell; Recombinant human KGF resulted in a increase in the proliferation and migration of CaSki cells; The proliferation and migration of CaSki cell became weak due to autocrine KGF neutralized by KGF antibodies (P < 0.05).


CONCLUSIONS
Current results demonstrate that KGF and KGFR express in CaSki cell; Both autocrine and recombinant human KGF have the effect on CaSki cell proliferation and migration.
Lu
Lin
L
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Wang
Dong
D
Hu
Li-na
LN
Chen
Bin
B
Jia
Hai-jun
HJ
Li
Jin-ke
JK
Wang
Deng-feng
DF
D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D051523:Fibroblast Growth Factor 7 / Q000378:metabolism*; D006801:Humans; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
C496453:FGF7 protein, human; D011994:Recombinant Proteins; D051523:Fibroblast Growth Factor 7; D051497:Receptor, Fibroblast Growth Factor, Type 2; C065104:keratinocyte growth factor receptor