Analysis of opioid peptides by on-line SPE-CE-ESI-MS.
In this study, SPE-CE-ESI-MS is explored for the preconcentration and separation of dilute solutions of six opioid peptides. First, a CE-ESI-MS methodology was developed and validated. LODs of around 1 microg/mL were obtained for all the studied peptides. For SPE-CE-ESI-MS experiments, a home-made SPE microcartridge containing a C18 sorbent was constructed near the inlet of the separation capillary. After optimizing the on-line preconcentration methodology, LODs between 10 and 0.1 ng/mL were achieved. Repeatability, reproducibility, durability of the microcartridges and linearity of the SPE-CE-ESI-MS methodology were also investigated and compared to the values obtained by CE-ESI-MS. Finally, human plasma samples fortified with opioid peptides were analyzed by SPE-CE-ESI-MS in order to show the potential of the methodology for the analysis of biological fluids.
Hernández
Elena
E
Department of Analytical Chemistry, University of Barcelona, Barcelona, Spain.
Benavente
Fernando
F
Sanz-Nebot
Victoria
V
Barbosa
José
J
D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D004745:Enkephalins / Q000302:isolation & purification; D006801:Humans; D009862:Online Systems; D018847:Opioid Peptides / Q000097:blood* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D002021:Buffers; D004745:Enkephalins; D018847:Opioid Peptides