[Bogotá paint-industry workers exposure to organic solvents and genotoxic effects].
OBJECTIVE
Investigating the degree of exposure to organic solvents and related genotoxic consequences in paint-factory workers by using cytogenetic monitoring and determining micronuclei frequency in lymphocytes and DNA damage as assessed by comet assays.


METHODS
Excretion of phenol and hippuric and methylhippuric acids in urine was investigated as biomarkers for benzene, toluene and xylene exposure. Urine samples were taken in two paint-factories in Bogotá and in a non-exposed control group. Benzene, toluene and xylene concentrations in air were measured in the work setting. Micronuclei and simple DNA chain breakage frequency were evaluated in peripheral blood sample mononuclear cells to assess early genetic effects.


RESULTS
Hippuric acid concentration was found to be within normal values; by contrast, phenol and methylhippuric acid sample concentrations were above normal values by 3,3 % and 50,8 %, respectively. Benzene concentration in air was above permissible limits in one of the factories. There were no statistical differences regarding genetic biomarkers between exposed and non-exposed workers.


CONCLUSIONS
Organic solvent levels in the samples were apparently low, which is consistent with the absence of genotoxicity in cells. This study represents a step forward in research in occupational health surveillance.
Cárdenas-Bustamante
Omayda
O
Laboratorio Salud Ambiental, Instituto Nacional de Salud, Bogotá.
Varona-Uribe
Marcela
M
Patiño-Florez
Rosa I
RI
Groot-Restrepo
Helena
H
Sicard-Suárez
Diana
D
Tórres-Carvajal
María M
MM
Pardo-Pardo
Darío
D
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D003105:Colombia / Q000453:epidemiology; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D006801:Humans; D007221:Industry; D008297:Male; D008875:Middle Aged; D009784:Occupational Diseases / Q000453:epidemiology* / Q000209:etiology*; D010150:Paint / Q000009:adverse effects*; D015995:Prevalence; D012997:Solvents / Q000009:adverse effects*
D012997:Solvents