[Study on parental origin of complete hydatidiform mole detected by polymorphisms of microsatellite].
OBJECTIVE
Through detection of 9 loci of polymorphisms of microsatellite to investigate the parental origin of complete hydatidiform mole.


METHODS
Using the technology and method of multipolymerase chain reaction and electrophoresis in denatured polyacrylamide gel and silver stain detection, we carried out DNA analysis on 50 cases of complete hydatidiform mole diagnosed by histopathology and 50 copies of the couples' peripheral blood.


RESULTS
There are 7 cases of biparental complete hydatidiform mole (14%); and 43 cases of androgenetic complete hydatidiform mole (86%) among 50 cases of complete hydatidiform mole.


CONCLUSIONS
Detection of polymorphisms of microsatellite is a technology that can be used to identify the parental origin of complete hydatidiform mole. It is simple, quick, reliable and highly efficient.
Deng
Shun-sheng
SS
Department of Gynecological Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
Ding
Zhi-ming
ZM
Qian
Jian-hua
JH
Lü
Wei-guo
WG
Wan
Xiao-yun
XY
Xie
Xing
X
D000328:Adult; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D006828:Hydatidiform Mole / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D007621:Karyotyping; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D011247:Pregnancy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014594:Uterine Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm