Modulation of endothelial responses by the stromal microenvironment: effects on leucocyte recruitment.
Vascular endothelial cells play a pivotal role in regulating leucocyte recruitment during inflammation, and their responses can be modulated by their local environment, including cells of the tissue stroma. We have developed a model system to examine how the communication between endothelial cells and fibroblasts regulates the recruitment of leucocytes and their subsequent subendothelial fate. Here, we describe a novel co-culture filter-based flow assay and highlight the ability of synovial fibroblasts obtained from chronically inflamed tissue to promote leucocyte recruitment to otherwise 'resting' endothelial cells.
McGettrick
H M
HM
Department of Physiology, The Medical School, The University of Birmingham, Birmingham B15 2TT, U.K. helenmcgettrick@yahoo.com
Filer
A
A
Rainger
G E
GE
Buckley
C D
CD
Nash
G B
GB
D018920:Coculture Techniques; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D007962:Leukocytes / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology*