title: Underlying mechanism of ASIC1a involved in acidosis-induced cytotoxicity in rat C6 glioma cells.
abstract: OBJECTIVE
  To investigate the underlying mechanism of acid-sensing ion channel (ASIC) 1a involved in the acidosis-induced cytotoxicity of rat C6 glioma cells.
  
  
  METHODS
  The stable ASIC1a-silenced C6 cells built with the RNA interference technology were confirmed by RT-PCR and Western blot analysis. Intracellular calcium ([Ca2+]i) in both the wild-type rat C6 glioma cells and the ASIC1a-silenced C6 cells were analyzed before and after acid application/exposure with the calcium imaging experiment.
  
  
  RESULTS
  The rapid extracellular pH drop induced the increase of [Ca2+]i in the wild-type C6 cells, but not in the ASIC1a-silenced C6 cells. During the prolonged acid exposure, [Ca2+]i was lower in the ASIC1a-silenced C6 cells than that in the control cells.
  
  
  CONCLUSIONS
  The resultant toxicity of [Ca2+]i might contribute to the acidosis-induced cytotoxicity.
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China. wengxc2002@yahoo.com.cn
authors.forename: Xie-chuan
authors.initials: XC
authors.lastname: Weng
authors.forename: Jian-quan
authors.initials: JQ
authors.lastname: Zheng
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Wen-bin
authors.initials: WB
authors.lastname: Xiao
mesh_terms: D062565:Acid Sensing Ion Channels; D000137:Acid-Base Imbalance / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D005910:Glioma / Q000378:metabolism*; D006851:Hydrochloric Acid / Q000008:administration & dosage / Q000633:toxicity*; D066298:In Vitro Techniques; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D015222:Sodium Channels / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D062565:Acid Sensing Ion Channels; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D015222:Sodium Channels; D006851:Hydrochloric Acid; D002118:Calcium