Activity of glycosidases (beta-N-acetyloglucosaminidase, alpha-mannosidase, and beta-galactosidase) in the uterine luminal fluid of cows after multiple ovulation.
This study investigated the activity of beta-N-acetyloglucosaminidase (beta-NAGASE), alpha-mannosidase, and beta-galactosidase in the uterine luminal fluid of cows after superovulation treatment, along with the possible associations between the activity of these 3 glycosidases and the superovulatory response. Embryos and a sample of fluid flushed from each uterine horn were collected on day 7 after artificial insemination (on estrus day 0) from 32 cows in which superovulation was induced with porcine follicle-stimulating hormone. Glycosidase activity was assayed colorimetrically. The cows were classified as to superovulatory response according to the number of corpora lutea per ovary (group 1, 1 to 4; group 2, > 4) and according to the total number of embryos per horn (T1, 0; T2, 1 to 2; T3, 3 to 4; T4, > 4) and the number of transferable embryos per horn (TR1, 0; TR2, 1 to 2; TR3, 3 to 4; TR4, > 4). The mean activity of beta-NAGASE was significantly lower (P < 0.05) in group 2 than in group 1, at 95.99 (standard error 20.43) versus 226.72 (46.77) IU/L. It was also significantly lower (P < 0.01) in group T4 compared with groups T1, T2, and T3, at 50.09 (8.21) versus 129.25 (34.60), 222.27 (62.62), and 290.26 (93.77) IU/L, respectively, as well as in group T1 compared with group T3. There was a positive relationship between beta-NAGASE activity and both the total number of embryos (P = 0.047) and the number of transferable embryos per horn (P = 0.013) when 1 to 4 corpora lutea developed per ipsilateral ovary. No difference in alpha-mannosidase or beta-galactosidase activity was detected among the groups.
Tsiligianni
Theodora
T
National Agricultural Research Foundation, Veterinary Research Institute of Thessaloniki, 57008 Ionia, Thessaloniki, Greece. tsiligianni@nagref.gr
Amiridis
Georgios S
GS
Vainas
Emmanuel
E
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D002417:Cattle / Q000196:embryology* / Q000502:physiology; D004624:Embryo Transfer / Q000662:veterinary*; D004971:Estrus; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007315:Insemination, Artificial / Q000662:veterinary; D010062:Ovulation Induction / Q000662:veterinary; D011247:Pregnancy; D011897:Random Allocation; D013480:Superovulation; D014599:Uterus / Q000201:enzymology*; D043323:alpha-Mannosidase / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D005640:Follicle Stimulating Hormone; D006026:Glycoside Hydrolases; D001616:beta-Galactosidase; D043323:alpha-Mannosidase; D000118:Acetylglucosaminidase