[Determination of cytoplasmic receptors for specific steroid hormones. II) Androgen receptor].
Cytoplasmic receptors for 5 alpha-dihydrotestosterone (3H-DHT) were determined in normal and hypertrophic human prostate using the slightly modified DCC method we previously standardized for 17beta-estradiol-receptor. Incubations were always performed at 0 degree C for 1 hr. Discrimination between 3H-DHT binding to cytoplasmic receptor and to Sex Hormone Binding Globulin (SHBG) was achieved on the basis of binding affinity, thermolability and pattern of specificity by various steroid hormones. In particular, 5 beta-DHT did not bind to cytoplasmic receptor, while it did to SHBG.
Nanni
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P
Istituto di Cancerologia dell'Università di Bologna.
Nicoletti
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G
De Giovanni
C
C
Galli
M C
MC
D003593:Cytoplasm / Q000737:chemistry; D003600:Cytosol / Q000737:chemistry*; D013196:Dihydrotestosterone / Q000032:analysis; D006801:Humans; D008297:Male; D011467:Prostate; D011944:Receptors, Androgen / Q000032:analysis*; D012738:Sex Hormone-Binding Globulin / Q000032:analysis
D011944:Receptors, Androgen; D012738:Sex Hormone-Binding Globulin; D013196:Dihydrotestosterone