title: Biomolecular interaction monitoring of autoantibodies by scanning surface plasmon resonance microarray imaging.
abstract: A new commercial surface plasmon resonance (SPR) imaging analysis system with a novel SPR dip angle scanning principle allows the measurement, without the need for labeling, of the exact SPR dip angle. With this system hundreds of biomolecular interactions can be monitored on microarrays simultaneously and with great precision. The potency of this system is demonstrated by automatically monitoring the interactions between citrullinated peptides and serum autoantibodies of 50 rheumatoid arthritis (RA) patients and 29 controls in a single step. The smallest antibody concentration that could be measured in this experimental setup was 0.5 pM.
authors.affiliation: Department of Biomolecular Chemistry, Nijmegen Centre for Molecular Life Sciences, Institute for Molecules and Materials, Radboud University Nijmegen, P. O. Box 9101, NL-6500 HB Nijmegen, The Netherlands.
authors.forename: Angelique M C
authors.initials: AM
authors.lastname: Lokate
authors.forename: J Bianca
authors.initials: JB
authors.lastname: Beusink
authors.forename: Geert A J
authors.initials: GA
authors.lastname: Besselink
authors.forename: Ger J M
authors.initials: GJ
authors.lastname: Pruijn
authors.forename: Richard B M
authors.initials: RB
authors.lastname: Schasfoort
mesh_terms: D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology; D001323:Autoantibodies / Q000097:blood / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D010455:Peptides / Q000097:blood / Q000737:chemistry* / Q000276:immunology; D040081:Protein Array Analysis / Q000379:methods*; D015203:Reproducibility of Results; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D001323:Autoantibodies; D010455:Peptides