Mammary gland homeostasis employs serotonergic regulation of epithelial tight junctions.
Homeostatic control of volume within the alveolar spaces of the mammary gland has been proposed to involve a feedback system mediated by serotonin signaling. In this article, we describe some of the mechanisms underlying this feedback based on studies of a human normal mammary epithelial cell line (MCF10A) and mouse mammary epithelium. Mammary serotonin was elevated during lactation and after injection of 5-hydroxytryptophan (5-HTP). The genes encoding the serotonin reuptake transporter (SERT) and the type 7 serotonin receptor (5-HT(7)) were expressed in human and mouse mammary epithelial cells, and serotonin caused a concentration-dependent increase of cAMP in MCF10A cells. Mouse and human mammary epithelial cells formed polarized membranes, in which tight junction activity was monitored. Treatment of mammary epithelial membranes with serotonin receptor antagonists increased their transepithelial electrical resistance (TEER). Antagonist and agonist effects on TEER were mediated by receptors on the basolateral face of the membranes. Our results suggest a process in which serotonin accumulates in the interstitial fluid surrounding the mammary secretory epithelium and is detected by 5-HT(7) receptors, whereupon milk secretion is inhibited. One mechanism responsible for this process is serotonin-mediated opening of tight junctions, which dissipates the transepithelial gradients necessary for milk secretion.
Stull
Malinda A
MA
Department of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, OH 45267-0576, USA.
Pai
Vaibhav
V
Vomachka
Archie J
AJ
Marshall
Aaron M
AM
Jacob
George A
GA
Horseman
Nelson D
ND
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D017097:Electric Impedance; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D006706:Homeostasis; D006801:Humans; D008321:Mammary Glands, Animal / Q000502:physiology* / Q000648:ultrastructure; D042361:Mammary Glands, Human / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D010539:Permeability; D011985:Receptors, Serotonin / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism* / Q000494:pharmacology; D012702:Serotonin Antagonists / Q000494:pharmacology; D050486:Serotonin Plasma Membrane Transport Proteins / Q000032:analysis / Q000378:metabolism; D017366:Serotonin Receptor Agonists / Q000494:pharmacology; D015398:Signal Transduction; D019108:Tight Junctions / Q000378:metabolism*
D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D050486:Serotonin Plasma Membrane Transport Proteins; D017366:Serotonin Receptor Agonists; C104425:serotonin 7 receptor; D012701:Serotonin