title: Nutritional regulation of keratinolytic activity in Bacillus pumilis.
abstract: Bacillus pumilis F3-4 utilized feather as a sole source of carbon, nitrogen and sulfur. Supplementation of the feather medium with glucose or MgSO(4) . 7H(2)O increased keratinolytic protease production (14.6-16.7 U/mg). The synthesis of keratinolytic protease was repressed by an exogenous nitrogen source. Keratinolytic protease was produced in the absence of feather (9.4 U/mg). Feather degradation resulted in sulfhydryl group formation (0.8-2.6 microM). B. pumilis F3-4 effectively degraded chicken feather (75%), duck feather (81%) and feather meal (97%), whereas human nails, human hair and sheep wool under went less degradation (9-15%).
authors.affiliation: School of Applied Life Science, Pusan National University, Miryang, Korea. shjoo@pusan.ac.kr
authors.forename: Hong-Joo
authors.initials: HJ
authors.lastname: Son
authors.forename: Hyean-Cheal
authors.initials: HC
authors.lastname: Park
authors.forename: Hong-Sung
authors.initials: HS
authors.lastname: Kim
authors.forename: Chung-Yeol
authors.initials: CY
authors.lastname: Lee
mesh_terms: D000818:Animals; D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D002645:Chickens; D003470:Culture Media; D005241:Feathers / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D007633:Keratins / Q000302:isolation & purification / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*
chemical_list: D003470:Culture Media; D007633:Keratins; D010447:Peptide Hydrolases; C035855:keratinase