title: Basic aspects of absorption and fluorescence spectroscopy and resonance energy transfer methods.
abstract: Absorption and fluorescence spectroscopy are the heart of life science laboratory techniques. These two spectroscopic tools are sensitive, are easy to use, and can provide a wide range of information. The goal of this chapter is to familiarize the reader with some basic principles and applications of absorption and steady-state fluorescence spectroscopy. Selected applications are illustrated with examples.
authors.affiliation: Department of Chemistry, State University of New York at Binghamton, Binghamton, New York 13902, USA.
authors.forename: Natasha
authors.initials: N
authors.lastname: Shanker
authors.forename: Susan L
authors.initials: SL
authors.lastname: Bane
mesh_terms: D000042:Absorption; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006835:Hydrazones / Q000737:chemistry; D012996:Solutions; D012997:Solvents; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014443:Tyrosine / Q000737:chemistry
chemical_list: D006835:Hydrazones; D012996:Solutions; D012997:Solvents; D014443:Tyrosine