title: The impact of dental surgery on HSV-1 reactivation in the oral mucosa of seropositive patients.
abstract: OBJECTIVE
  Herpes simplex virus type 1 (HSV-1) is a member of the human herpes virus family. Most of the population (90%) has antibodies to HSV-1, and as many as 40% of these individuals may develop secondary herpes. Shedding of HSV-1 in the oral mucosa can be induced by many factors, including stress, sunlight, menstruation, and physical trauma. The aim of the present study was to evaluate the impact of dental surgical procedures on HSV-1 shedding in the oral mucosa.
  
  
  METHODS
  The case group comprised 48 patients undergoing third molar extraction (case group) and 48 patients undergoing conventional restorative procedures (control group). All of the patients were IgG-positive for HSV-1. Oral swabs were performed before and 1 week after the procedures to investigate HSV-1 reactivation by nested polymerase chain reaction.
  
  
  RESULTS
  The frequency of positive oral swabs to HSV-1 in the group that underwent surgery (4.2%) was not statistically different from that in the control group (2.1%).
  
  
  CONCLUSIONS
  The results indicate that oral surgical trauma does not have a significant impact on HSV-1 shedding in the oral mucosa.
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: Marques-Silva
authors.forename: Wagner Henriques
authors.initials: WH
authors.lastname: Castro
authors.forename: Eliane Lustosa Cabral
authors.initials: EL
authors.lastname: Gomez
authors.forename: André Luiz Sena
authors.initials: AL
authors.lastname: Guimarães
authors.forename: Marina Sena Lopes
authors.initials: MS
authors.lastname: Silva
authors.forename: Ricardo Santiago
authors.initials: RS
authors.lastname: Gomez
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D004279:DNA, Viral / Q000032:analysis; D003793:Dental Restoration, Permanent; D004587:Electrophoresis, Agar Gel; D005260:Female; D005500:Follow-Up Studies; D006560:Herpes Labialis / Q000821:virology; D018259:Herpesvirus 1, Human / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008964:Molar, Third / Q000601:surgery; D009061:Mouth Mucosa / Q000821:virology*; D016133:Polymerase Chain Reaction; D013283:Stomatitis, Herpetic / Q000821:virology*; D014081:Tooth Extraction; D014095:Tooth, Impacted / Q000601:surgery; D014775:Virus Activation / Q000502:physiology*; D017201:Virus Shedding / Q000502:physiology
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G