title: Use of cyclic voltammetry, photochemiluminescence, and spectrophotometric methods for the measurement of the antioxidant capacity of buckwheat sprouts.
abstract: This paper describes the use of cyclic voltammetry, photochemiluminescence (PCL), and spectrophotometric methods (TEAC and FCR reducing capacity) for the measurement of the antioxidant capacity of dark- and light-grown buckwheat sprouts. Moreover, the flavonoid profile of studied material is provided. Methanol extracts (80%) from ungerminated buckwheat grain and 6 and 8 DAS (days after seeding) sprouts were used. The 8 day germination period was sufficient to obtain good quality sprouts with completely removed pericarps. The ungerminated buckwheat grain contained only rutin, whereas in sprouts produced in dark or light, a high level of isoorientin, orientin, vitexin, rutin, and isovitexin was found. The flavonoid content in sprouts produced under light was almost 2 times higher than those of sprouts produced in the dark. The antioxidant capacity of light-grown sprouts was higher than that of dark-grown ones. The results from voltammetric experiments obtained for buckwheat seeds and 6 and 8 DAS sprouts harvested under dark or light conditions highly correlated with those obtained by PCL antioxidant capacity of water-soluble substances (PCL ACW) (r = 0.99), PCL antioxidant capacity of lipid-soluble substances (r = 0.99), TEAC (r = 0.99), and FCR reducing capacity (r = 0.99). The use of cyclic voltammetry, PCL ACW, and TEAC was fully applicable for the evaluation of the antioxidant capacity of buckwheat sprouts.
authors.affiliation: University of Warmia and Mazury, Olsztyn, Poland. dziel@uwm.edu.pl
authors.forename: Danuta
authors.initials: D
authors.lastname: Zielinska
authors.forename: Dorota
authors.initials: D
authors.lastname: Szawara-Nowak
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ornatowska
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Wiczkowski
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods*; D019612:Fagopyrum / Q000737:chemistry*; D018525:Germination; D010084:Oxidation-Reduction; D010777:Photochemistry / Q000379:methods*; D012639:Seeds / Q000737:chemistry; D012995:Solubility; D013053:Spectrophotometry / Q000379:methods*; D013997:Time Factors
chemical_list: D000975:Antioxidants