title: Genetic screen for regulators of ind expression identifies shrew as encoding a novel twisted gastrulation-like protein involved in Dpp signaling.
abstract: Initiation and refinement of expression of the Ind homeodomain protein in the Drosophila embryo is coordinately regulated by global dorsoventral patterning pathways Dorsal, Egfr, and Dpp, and well as by Vnd, which positions the ventral boundary of Ind. Therefore, we set out to look for novel regulators of dorsoventral patterning by screening the Exelixis deficiency collection for modified expression of Ind. Indeed, we found deficiencies that remove components of the known signaling pathways had altered or lost ind expression. These findings included deficiencies that remove screw, dpp, and egfr as well as deficiencies that remove ind itself. In addition, we found several deficiencies that had altered or loss of ind expression. We also observed phenotypes suggestive of dorsoventral patterning defects such as twisting during gastrulation, and defects associated with loss of dorsal specification. These include a pair of overlapping deficiencies that produced ventralized embryos. We find that transheterozygotes of these two deficiencies are also ventralized. There are seven genes common to both deficiencies, including CG11582, which encodes a twisted gastrulation-like protein. These two deficiencies are also allelic with shrew mutations. Here, we present data supporting the conclusion that CG11582 is the gene affected in shrew mutants.
authors.affiliation: Division of Biology, Kansas State University, Manhattan, Kansas 66506, USA.
authors.forename: Morgan
authors.initials: M
authors.lastname: Bonds
authors.forename: Jennifer
authors.initials: J
authors.lastname: Sands
authors.forename: Will
authors.initials: W
authors.lastname: Poulson
authors.forename: Canda
authors.initials: C
authors.lastname: Harvey
authors.forename: Tonia
authors.initials: T
authors.lastname: Von Ohlen
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004330:Drosophila / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics; D005260:Female; D054262:Gastrulation / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D018398:Homeodomain Proteins / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D011494:Protein Kinases / Q000235:genetics; D018010:Receptors, Invertebrate Peptide / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
chemical_list: D029721:Drosophila Proteins; D018398:Homeodomain Proteins; C116352:IND protein, Drosophila; D018010:Receptors, Invertebrate Peptide; C090223:TSG protein, Drosophila; C064196:dpp protein, Drosophila; D011494:Protein Kinases; C066532:Egfr protein, Drosophila; D066246:ErbB Receptors