[A technique for investigating the component of cantharidin blisters by flow cytometry].
OBJECTIVE
Cantharidimide cause blister. The effect of blister on immunoregulation was investigated.


METHODS
Cantharidimide was placed on the skin, 48h later, the blister was analyzed by flow cytometry.


RESULTS
The blister contained 1 x 10(6) - 1 x 10(7) cells per ml, most of which were neutrophils, macrophages, dendritic cells (DC), and IL-12 secreted by Thl cells.


CONCLUSIONS
There are high concent of DC in the blister, which is differential and induce the secretion of Th1, the activation of T cell. The blister modulate the biological response of patients and is helpful for treatment with infective disease.
Zou
Xiao-jun
XJ
Department of Infectious Diseases, Communicable Diseases Center of PLA, The 458th Hospital of PLA, Guangzhou 510602, China. kyzouxj@163.com
Liu
Hui-ping
HP
Cheng
Tao
T
Shi
Li
L
Zu
Ping
P
Zhang
Yi-jun
YJ
D000328:Adult; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D001768:Blister / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D001826:Body Fluids / Q000166:cytology / Q000276:immunology; D002193:Cantharidin / Q000506:poisoning*; D001517:Coleoptera / Q000737:chemistry; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D018664:Interleukin-12 / Q000096:biosynthesis; D007509:Irritants / Q000506:poisoning; D008297:Male; D008419:Materia Medica / Q000737:chemistry*; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
D015703:Antigens, CD; D007509:Irritants; D008419:Materia Medica; D018664:Interleukin-12; D002193:Cantharidin