p53 protein expression levels as bioindicator of individual exposure to ionizing radiation by flow cytometry.
Ionizing radiation (IR) can cause various lesions in DNA, which induce the increase of p53 expression levels in order to repair radiation induced damage. Thus, the correlation between the increase of p53 expression and an irradiation may constitute a fast and powerful method of individual monitoring in cases of accidental or suspected exposures to IR. In this context, the aim of this research was to evaluate changes in lymphocyte p53 expression levels, based on flow cytometry, after in vitro irradiation of peripheral blood samples. For the measurement of such expression levels of p53 protein, an investigation was carried out in order to establish a methodology of analysis based on flow cytometry. Hence, relationships among levels of expression of p53 protein with the absorbed dose have been verified. The results presented in this report emphasized flow cytometry as an important tool for the fast evaluation of p53 protein expression levels as bioindicator of individual exposure to acute ionizing radiation.
Cavalcanti
Mariana Brayner
MB
Grupo de Estudos em Radioproteção e Radioecologia, Departamento de Energia Nuclear, Universidade Federal de Pernambuco, Avenida Professor Luiz Freire, 1000, Cidade Universitária, PE 50740-540, Brazil. maribrayner@yahoo.com.br
de Jesus Amaral
Ademir
A
de Salazar E Fernandes
Thiago
T
Melo
Jonathan Azevedo
JA
de Faria Machado
Cintia Gonsalves
CG
D004307:Dose-Response Relationship, Radiation; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects; D008297:Male; D011839:Radiation, Ionizing; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D016159:Tumor Suppressor Protein p53