Selective detection of extracellular glutamate in brain tissue using microelectrode arrays coated with over-oxidized polypyrrole.
We describe the selective measurement of extracellular glutamate concentration in rodent brain using ceramic-based platinum microelectrode arrays (MEAs) coated with electropolymerized, over-oxidized polypyrrole (OPP) as a permselective barrier to interference from dopamine and ascorbate. OPP-coated MEAs displayed similar sensitivity (97 +/- 9 nA microM(-1) cm(-2)) and response time (ca. 1 s) to glutamate as previously described Nafion-coated MEAs but, unlike Nafion-coated MEAs, were unresponsive to 5 microM dopamine. Such OPP-coated MEAs afforded selective detection of evoked glutamate release in the dopamine-rich striatum of the mouse brain.
Walker
Eric
E
Department of Psychiatry and Biobehavioral Sciences, Semel Institute for Neuroscience & Human Behavior, University of California, Los Angeles, CA 90095, USA.
Wang
Jianjun
J
Hamdi
Naser
N
Monbouquette
Harold G
HG
Maidment
Nigel T
NT
D000818:Animals; D001923:Brain Chemistry; D004563:Electrochemistry / Q000379:methods; D005110:Extracellular Space / Q000737:chemistry*; D005971:Glutamates / Q000032:analysis*; D006801:Humans; D008839:Microelectrodes; D011108:Polymers; D011758:Pyrroles
D005971:Glutamates; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole