Reduction of cis,trans,cis-[PtCl2(OCOCH3)2(NH3)2] by aqueous extracts of cancer cells.
Pt(IV) complexes must be reduced to kill cancer cells. While reduction rates correlate with reduction potentials, we wanted to check if the rates of reduction depend on the cell line used. The reduction of cis,trans,cis-[PtCl2(OCOCH3)2(NH3)2] by extracts of three cell lines was measured, and the rates follow the order A2780cisR > A2780 > HT-29. The reduction is not carried out by the low molecular weight (MW) antioxidants but primarily by cellular components with MW > 3000.
Nemirovski
Alina
A
Department of Medicinal Chemistry and Natural Products, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem, 91120, Israel.
Kasherman
Yonit
Y
Tzaraf
Yael
Y
Gibson
Dan
D
D000970:Antineoplastic Agents / Q000737:chemistry*; D000975:Antioxidants / Q000378:metabolism; D001685:Biological Factors / Q000737:chemistry*; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D002457:Cell Extracts / Q000737:chemistry*; D002945:Cisplatin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D005978:Glutathione / Q000378:metabolism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009944:Organoplatinum Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D014407:Tumor Cells, Cultured; D014867:Water
D000970:Antineoplastic Agents; D000975:Antioxidants; D001685:Biological Factors; D002457:Cell Extracts; D009944:Organoplatinum Compounds; C525456:diamminedichlorodiacetatoplatinum(II); D014867:Water; D019328:Buthionine Sulfoximine; D005978:Glutathione; D002945:Cisplatin