Dietary 1,3-diacylglycerol protects against diet-induced obesity and insulin resistance.
To investigate the effect of dietary 1,3-diacylglycerol (DAG) on the development of insulin resistance (IR) and obesity, brown adipose tissue-deficient mice, a model of high-fat diet-induced IR and obesity, were fed Western-type diets (WTD) containing either DAG oil (n = 8) or standard triacylglycerol (TAG) oil (n = 9) for 15 weeks, beginning at 8 weeks of age. Although brown adipose tissue-deficient mice became obese on both TAG- and DAG-enriched WTD (TAG-WTD and DAG-WTD), the mice eating DAG-WTD gained less weight and had less body fat accumulation. The results of glucose tolerance tests conducted after 5 weeks of each WTD were not different. However, after 10 weeks of each WTD, impaired glucose tolerance developed in the TAG-WTD group but was prevented by DAG-WTD. Exploratory analyses of gene expression suggested that consumption of DAG-WTD was associated with reduced phosphoenolpyruvate carboxykinase gene expression in liver and increased expression of the genes for peroxisome proliferator-activated receptor alpha, lipoprotein lipase, and uncoupling proteins 2 and 3 in skeletal muscle. There were no effects of the DAG-WTD on fasting and postprandial plasma triglyceride (TG) levels, hepatic TG content, or the rate of secretion of TG from the liver. These findings suggest that diets enriched in 1,3-DAG oil may reduce WTD-induced IR and body fat accumulation by suppressing gluconeogenesis in liver and stimulating fat oxidation in skeletal muscle.
Saito
Shinichiro
S
The Division of Preventive Medicine & Nutrition, Department of Medicine, College of Physicians & Surgeons, Columbia University, New York, NY 10032, USA.
Hernandez-Ono
Antonio
A
Ginsberg
Henry N
HN
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004032:Diet; D004075:Diglycerides / Q000008:administration & dosage / Q000494:pharmacology*; D005951:Glucose Tolerance Test; D007333:Insulin Resistance; D051379:Mice; D009765:Obesity / Q000517:prevention & control*; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D004075:Diglycerides