title: Altered gene expression by cisplatin in a human squamous cell lung carcinoma cell line.
abstract: BACKGROUND
  Substantial evidence has disclosed that some cytotoxic agents have complex activities in influencing signal transduction pathways in cells.
  
  
  METHODS
  cDNA microarray analysis was performed after exposing a human squamous cell carcinoma cell line, RERF-LC-AI, to low-dose cisplatin for 5 days. Up-regulated gene expressions were suppressed by small interfering RNA to investigate phenotypic alteration of the cells.
  
  
  RESULTS
  Among 30,000 genes screened, 42 genes showed increases or decreases in expression of more than 2-fold with cisplatin treatment. They included genes with functions involved in apoptosis, cell cycle regulation and DNA metabolism/repair. Suppression of the 5 most significantly altered genes by small interfering RNA resulted in partly reduced apoptosis without altering cytotoxicity of cisplatin.
  
  
  CONCLUSIONS
  Besides direct cytotoxic effects on cells, cisplatin may have indirect effects involving drug resistance, and synergistic effects with other agents.
authors.affiliation: Department of Respirology, Graduate School of Medicine, Chiba University, Chiba, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Yatomi
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Takiguchi
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Asaka-Amano
authors.forename: Makoto
authors.initials: M
authors.lastname: Arai
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tada
authors.forename: Katsushi
authors.initials: K
authors.lastname: Kurosu
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Sakao
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Kasahara
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Tanabe
authors.forename: Koichiro
authors.initials: K
authors.lastname: Tatsumi
authors.forename: Naohiko
authors.initials: N
authors.lastname: Seki
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kuriyama
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000235:genetics* / Q000378:metabolism; D002945:Cisplatin / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000970:Antineoplastic Agents; D034741:RNA, Small Interfering; D002945:Cisplatin