Bone histomorphometry in arthritis models.
Rodent arthritis models are tools to study joint destruction. This chapter describes methods to prepare and analyze histological sections from inflamed paws. Protocols to create decalcified paraffin-embedded as well as undecalcified plastic-embedded sections are provided. Strengths and limitations of these two histological techniques are described. Major staining techniques on hard tissue sections focusing on the evaluation of joint destruction are indicated. Finally, evaluation of arthritic paw sections by histomorphometry, a semiautomated computerized method to measure areas of interest, is described.
Schett
Georg
G
Tuerk
Birgit
B
D000818:Animals; D001169:Arthritis, Experimental / Q000473:pathology*; D001842:Bone and Bones / Q000473:pathology*; D003648:Decalcification Technique / Q000379:methods; D006652:Histological Techniques / Q000379:methods*; D007596:Joints / Q000473:pathology; D051379:Mice; D016612:Paraffin Embedding / Q000379:methods; D016613:Plastic Embedding / Q000379:methods; D051381:Rats; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation / Q000379:methods