title: [Selection of attenuated Vibrio cholerae strains to obtain oral attenuated candidate vaccines against cholera].
abstract: A methodology was developed for the selection of genetically modified strains of Vibrio cholerae 01 and 0139 aimed at obtaining oral attenuated candidate vaccines against cholera. The modified strains underwent microbiological characterization, bacterial susceptibility and different biological tests (mean lethal dose, colonizing capacity, adherence in mice, ligated intestine and intraduodenal inoculation in rabbits as virulence and potency tests. The strains 81, 638, 638T and 1333 were evaluated in clinical trials to determine their reactogenicity and immunogenicity. All the strains were sensitive to tetracycline and doxoclycine. They showed their attenuation and immunogenicity in animal models. The strains 638 and 1333 proved to be immunogenic and non reactogenic in volunteers.
authors.affiliation: Instituto Finlay, Ciudad de La Habana, Cuba. hgarcia@finlay.edu.cu
authors.forename: Hilda
authors.initials: H
authors.lastname: María García
authors.forename: Gemma
authors.initials: G
authors.lastname: Año
authors.forename: Bárbara
authors.initials: B
authors.lastname: Cedré
authors.forename: Tania
authors.initials: T
authors.lastname: Valmaseda
authors.forename: Jorge Luis
authors.initials: JL
authors.lastname: Maestre
authors.forename: Manuel
authors.initials: M
authors.lastname: Díaz
authors.forename: Mayelin
authors.initials: M
authors.lastname: Mirabal
authors.forename: Arturo
authors.initials: A
authors.lastname: Talavera
authors.forename: Luis
authors.initials: L
authors.lastname: García
mesh_terms: D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D002771:Cholera / Q000517:prevention & control*; D022121:Cholera Vaccines / Q000008:administration & dosage / Q000009:adverse effects / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D003627:Data Interpretation, Statistical; D004318:Doxycycline / Q000494:pharmacology; D006801:Humans; D051379:Mice; D046529:Microscopy, Electron, Transmission; D023421:Models, Animal; D011817:Rabbits; D013752:Tetracycline / Q000494:pharmacology; D014613:Vaccines, Attenuated; D014734:Vibrio cholerae / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D022121:Cholera Vaccines; D014613:Vaccines, Attenuated; D013752:Tetracycline; D004318:Doxycycline