title: Prevalence and first genetic identification of Cryptosporidium spp. in cattle in central Viet Nam.
abstract: We investigated the prevalence of Cryptosporidium infection in relation to age and clinical status in cattle in the central region of Viet Nam. A total of 266 fecal samples from diarrheic and non-diarrheic cattle were examined by the modified Ziehl-Neelsen staining method. Prevalence of Cryptosporidium parvum type infections, those of the Cryptosporidium andersoni type, and mixed infection of both types was 33.5% (89/266), 5.6% (15/266), and 3.4% (9/266), respectively. The infection rate of 44.3% (35/79) of C. parvum in calves less than 6 months old was significantly higher than that of 28.9% (54/187) in cattle greater than 6 months old (P<0.01). Although no C. andersoni oocysts were detected in calves less than 3 months old, no significant difference was observed between the age groups in the prevalence of C. andersoni infection and mixed infection. The percentage of diarrheic and non-diarrheic cattle identified to be shedding C. parvum oocysts was 46.5% (74/159) and 14.0% (15/107), respectively (P<0.0001). The risk of diarrhea was 1.7 times greater in C. parvum-infected calves than in their non-infected counterparts. DNA sequences of 18S rRNA genes of C. parvum type and C. andersoni type indicated that they were C. parvum bovine genotype and C. andersoni, respectively. This is the first genetic identification of C. parvum bovine genotype and C. andersoni from cattle in Viet Nam.
authors.affiliation: Department of Parasitology, Central Viet Nam Veterinary Institute, km4 Dong De Street, Nha Trang, Viet Nam.
authors.forename: Sam Thi
authors.initials: ST
authors.lastname: Nguyen
authors.forename: Duc Tan
authors.initials: DT
authors.lastname: Nguyen
authors.forename: Duc Quyet
authors.initials: DQ
authors.lastname: Le
authors.forename: Luc Ngoc
authors.initials: LN
authors.lastname: Le Hua
authors.forename: Thoai
authors.initials: T
authors.lastname: Van Nguyen
authors.forename: Hajime
authors.initials: H
authors.lastname: Honma
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakai
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000469:parasitology; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000032:analysis*; D005243:Feces / Q000469:parasitology; D005838:Genotype; D033661:Oocysts / Q000254:growth & development; D010270:Parasite Egg Count / Q000662:veterinary; D015995:Prevalence; D013045:Species Specificity; D014744:Vietnam / Q000453:epidemiology
chemical_list: D016054:DNA, Protozoan