Inhibitory effects of thunberginols A and B isolated from Hydrangeae Dulcis Folium on mRNA expression of cytokines and on activation of activator protein-1 in RBL-2H3 cells.
Previously, thunberginols A and B from the processed leaves of Hydrangeae macrophylla var. thunbergii (Hydrangea dulcis folium) substantially inhibited the degranulation caused by antigen and calcium ionophore A23187, and the release of tumor necrosis factor (TNF)-alpha and interleukin (IL)-4 by antigen in RBL-2H3 cells. In the present study, we examined the effect of thunberginol B on the expression of mRNA of several cytokines [ILs-2, 3, 4 and 13, TNF-alpha and granulocyte/macrophage-colony stimulating factor (GM-CSF)] and effects of thunberginols A and B on activator protein (AP)-1 composed of c-jun and c-fos, which is essential for the expression of the cytokine mRNA, in RBL-2H3 cells. Thunberginol B inhibited up-regulated genes of all cytokines, and thunberginols A and B (30 microM) inhibited the phosphorylation of c-jun and expression of c-fos mRNA and phosphorylation of extracellular signal-regulated kinases (ERK1/2). In addition, the profile of gene expression by thunberginol B was similar to that by luteolin, a natural flavone with a potent anti-allergic effect.
Matsuda
Hisashi
H
Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8412, Japan.
Wang
Qilong
Q
Matsuhira
Koudai
K
Nakamura
Seikou
S
Yuan
Dan
D
Yoshikawa
Masayuki
M
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D001578:Benzopyrans / Q000494:pharmacology*; D000001:Calcimycin / Q000276:immunology; D045744:Cell Line, Tumor; D016207:Cytokines / Q000096:biosynthesis*; D004140:Dinitrophenols / Q000276:immunology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects*; D016762:Genes, fos / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000187:drug effects; D031966:Hydrangea / Q000737:chemistry*; D049934:Isocoumarins / Q000494:pharmacology*; D047311:Luteolin / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D012710:Serum Albumin, Bovine / Q000276:immunology; D013997:Time Factors; D018808:Transcription Factor AP-1 / Q000502:physiology*
C516820:9-(3,4-dihydro-2,8-dimethyl-6-hydroxy-2H-1-benzopyran-2-yl)-6-methyl-2-(4-methyl-3-pentenyl)-2,6-nonadienoic acid; D018926:Anti-Allergic Agents; D001578:Benzopyrans; D016207:Cytokines; D004140:Dinitrophenols; D049934:Isocoumarins; D010936:Plant Extracts; D012333:RNA, Messenger; D018808:Transcription Factor AP-1; C034638:dinitrophenyl-bovine serum albumin; C079056:thunberginol B; D012710:Serum Albumin, Bovine; D000001:Calcimycin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D048049:Extracellular Signal-Regulated MAP Kinases; D047311:Luteolin