Targeted inhibition of Pdp1epsilon abolishes the circadian behavior of Drosophila melanogaster.
vrille and Par domain protein 1 (Pdp1) epsilon constitute the second transcriptional feedback loop in Drosophila circadian clock system. Their rhythmic expression is controlled by Drosophila Clock (dClk) gene, and they feed back to negatively and positively, respectively, regulate the oscillating transcription from dClk gene. In this study, we characterized the functional domains of PDP1epsilonin vitro using a panel of deletion mutants and showed that PDP1epsilon basic leucine zipper domain can act as a dominant-negative (DN) mutant of wild-type PDP1epsilon. In transgenic flies, the inhibition of PDP1epsilon activity by PDP1(DN) expression or PDP1 knock-down resulted in arrhythmic circadian behavior with altered dorsal projections from small ventral lateral neurons. We propose that one of PDP1-target genes may be involved in the formation of neural connection between the pacemaker cells and their targets for maintaining the rhythmicity of adult locomotor activity under free-running condition.
Lim
Chunghun
C
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
Lee
Jongbin
J
Koo
Eunjin
E
Choe
Joonho
J
D000818:Animals; D030801:Animals, Genetically Modified; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000502:physiology*; D056926:CLOCK Proteins; D002478:Cells, Cultured; D002940:Circadian Rhythm; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology*; D008124:Locomotion; D009154:Mutation; D009474:Neurons / Q000502:physiology; D017434:Protein Structure, Tertiary; D014157:Transcription Factors / Q000502:physiology
D050976:Basic-Leucine Zipper Transcription Factors; C120526:Clk protein, Drosophila; D029721:Drosophila Proteins; C109768:PDP1 protein, Drosophila; D014157:Transcription Factors; D056926:CLOCK Proteins