title: Hepatoprotective and Antioxidant Effects of Ethanol Extract from Phellinus merrillii on carbon tetrachloride-induced liver damage.
abstract: In the present study, we investigated the hepatoprotective and antioxidant capacities of ethanol extract of Phellinus merrillii (PM) on carbon tetrachloride-induced hepatotoxicity. In high-performance liquid chromatography (HPLC) analysis, the finger print chromatogram of PM was established. Both hispolon and PM showed a similar peak at the retention time of 6 min. This implied that PM did contain the active ingredient of hispolon. Treatment with PM (0.5, 1 and 2 g/kg) prior to the administration of carbon tetrachloride (1.5 ml/kg in olive oil, 20%) significantly prevented the increased serum alanine aminotransferase (s-GOT) and serum aspartate aminotransferase (s-GPT) in a dose-dependent manner. We also found that the incidences of ballooning degeneration, necrosis and portal triaditis were lowered in the group pretreated with PM. Carbon tetrachloride induces up-regulation of antioxidant enzymes, including superoxide dismutase (SOD) (86.6%), catalase (58.8%) and glutathione peroxidase (GPx)(64.7%) in the liver. Pretreatment with PM significantly reduced the all these antioxidant enzyme activities. Therefore, we verified that ethanol extract of PM has the hepatoprotective and antioxidant capacities on rats.
authors.affiliation: Institute of Chinese Pharmaceutical Sciences, College of Pharmacy, China Medical University, 91, Hsueh-Shih Road, Taichung 404, Taiwan.
authors.forename: Heng-Yuan
authors.initials: HY
authors.lastname: Chang
authors.forename: Wen-Huang
authors.initials: WH
authors.lastname: Peng
authors.forename: Ming-Jyh
authors.initials: MJ
authors.lastname: Sheu
authors.forename: Guan-Jhong
authors.initials: GJ
authors.lastname: Huang
authors.forename: Mu-Chuan
authors.initials: MC
authors.lastname: Tseng
authors.forename: Ming-Tsung
authors.initials: MT
authors.lastname: Lai
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Ho
authors.forename: Yuan Shiun
authors.initials: YS
authors.lastname: Chang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D002252:Carbon Tetrachloride Poisoning / Q000201:enzymology / Q000503:physiopathology / Q000517:prevention & control*; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000503:physiopathology / Q000517:prevention & control*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry*; D008111:Liver Function Tests; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D000431:Ethanol