Effect of UV irradiation on stabilized collagen: role of chromium(III).
Research on the effect of UV radiation on stabilized collagen is an area of potential interest owing to the fact that collagen is an important biomaterial finding immense use in various fields. In this present study, effect of UV irradiation on collagen stabilized using chromium(III) has been studied. The physical and optical properties affected by UV irradiation have been detailed. Viscosity measurements have shown that chromium(III) treated collagen has better stability against UV radiation than native collagen. Circular dichroic studies indicate that increase in concentration of chromium(III) does not affect the conformation of collagen however, the duration of irradiation has profound impact on the conformation of collagen. The fluorescence intensity of native collagen has been found to decrease more than that of chromium(III) treated collagen. The difference absorption spectra also shows that chromium(III) treatment brings about more stability to collagen against UV irradiation.
Fathima
Nishtar Nishad
NN
Chemical Lab, Central Leather Research Institute, Adyar, Chennai 20, India.
Suresh
Raju
R
Rao
Jonnalagadda Raghava
JR
Nair
Balachandran Unni
BU
Ramasami
Thirumalachari
T
D000818:Animals; D017608:Chromium Compounds / Q000494:pharmacology*; D002942:Circular Dichroism; D003094:Collagen / Q000187:drug effects / Q000528:radiation effects*; D008297:Male; D011487:Protein Conformation / Q000187:drug effects; D051381:Rats; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013431:Sulfates / Q000494:pharmacology*; D014466:Ultraviolet Rays; D014783:Viscosity
D017608:Chromium Compounds; D013431:Sulfates; C040874:chromium sulfate; D003094:Collagen