title: Multilocus sequence typing analysis of Shigella flexneri isolates collected in Asian countries.
abstract: The multilocus sequence typing scheme used previously for phylogenetic analysis of Escherichia coli was applied to 107 clinical isolates of Shigella flexneri. DNA sequencing of 3423 bp throughout seven housekeeping genes identified eight new allele types and ten new sequence types among the isolates. S. flexneri serotypes 1-5, X and Y were clustered together in a group containing many allelic variants while serotype 6 formed a distinct group, as previously established.
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea.|International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul 151-818, Republic of Korea.
authors.forename: Seon Young
authors.initials: SY
authors.lastname: Choi
authors.affiliation: Interdisciplinary Program of Bioinformatics, Seoul National University, Seoul 151-742, Republic of Korea.|International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul 151-818, Republic of Korea.
authors.forename: Yoon-Seong
authors.initials: YS
authors.lastname: Jeon
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea.|International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul 151-818, Republic of Korea.
authors.forename: Je Hee
authors.initials: JH
authors.lastname: Lee
authors.affiliation: International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul 151-818, Republic of Korea.
authors.forename: Boram
authors.initials: B
authors.lastname: Choi
authors.affiliation: International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul 151-818, Republic of Korea.
authors.forename: Sun Hwa
authors.initials: SH
authors.lastname: Moon
authors.affiliation: International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul 151-818, Republic of Korea.
authors.forename: Lorenz
authors.initials: L
authors.lastname: von Seidlein
authors.affiliation: International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul 151-818, Republic of Korea.
authors.forename: John D
authors.initials: JD
authors.lastname: Clemens
authors.affiliation: The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire CB10 1SA, UK.
authors.forename: Gordon
authors.initials: G
authors.lastname: Dougan
authors.affiliation: The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire CB10 1SA, UK.
authors.forename: John
authors.initials: J
authors.lastname: Wain
authors.affiliation: The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire CB10 1SA, UK.
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.affiliation: School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
authors.forename: Je Chul
authors.initials: JC
authors.lastname: Lee
authors.affiliation: School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
authors.forename: Sung Yong
authors.initials: SY
authors.lastname: Seol
authors.affiliation: Laboratory of Enteric Infections, Department of Microbiology, National Institute of Health Korea, Republic of Korea.
authors.forename: Bok Kwon
authors.initials: BK
authors.lastname: Lee
authors.affiliation: Division of Infectious Disease, Samsung Medical Center, Seoul, Republic of Korea.
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Song
authors.affiliation: International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul 151-818, Republic of Korea.
authors.forename: Manki
authors.initials: M
authors.lastname: Song
authors.affiliation: International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul 151-818, Republic of Korea.
authors.forename: Cecil
authors.initials: C
authors.lastname: Czerkinsky
authors.affiliation: Interdisciplinary Program of Bioinformatics, Seoul National University, Seoul 151-742, Republic of Korea.|School of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea.|International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul 151-818, Republic of Korea.
authors.forename: Jongsik
authors.initials: J
authors.lastname: Chun
authors.affiliation: International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul 151-818, Republic of Korea.
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Kim
mesh_terms: D000483:Alleles; D001208:Asia; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004405:Dysentery, Bacillary / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D012763:Shigella flexneri / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial