Preparation, application and testing of permanent antibacterial and antiviral coatings.
Protocols for the synthesis of the microbicidal polycation N,N-dodecyl,methyl-polyethylenimine and coating (painting) of glass slides with this polycation's butanol solution are described. Subsequently detailed are the procedures for validating that the resultant coated slides are essentially 100% lethal to the human bacterial pathogens, Staphylococcus aureus and Escherichia coli, as well as to two common strains of influenza virus. The time required to prepare and apply the cationic polymer and to test its microbicidal efficiency is conservatively estimated to be <4 weeks.
Haldar
Jayanta
J
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Weight
Alisha K
AK
Klibanov
Alexander M
AM
D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D000998:Antiviral Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D005898:Glass; D008826:Microbial Sensitivity Tests; D009975:Orthomyxoviridae / Q000187:drug effects; D011094:Polyethyleneimine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties
D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; C525941:N,N-dodecyl,methyl-polyethylenimine; D011094:Polyethyleneimine