Improvement of RT-PCR detection of chronic bee paralysis virus (CBPV) required by the description of genomic variability in French CBPV isolates.
A new RT-PCR test has been developed to diagnose Chronic bee paralysis virus (CBPV) that is able to detect genetically variable viral isolates. In fact, up to 8.7% divergence between partial nucleotide sequences from viral isolates from French honey bees was highlighted in a preliminary variability study. The previously-described RT-PCR was unable to detect all these viral isolates and RT-PCR diagnosis needed improvement. The new RT-PCR test can detect up to 40% more CBPV isolates.
Blanchard
Philippe
P
Agence Française de Sécurité Sanitaire des Aliments (AFSSA) Sophia-Antipolis, Unité Pathologie de l'Abeille, Les Templiers, France. p.blanchard@afssa.fr
Olivier
Violaine
V
Iscache
Anne-Laure
AL
Celle
Olivier
O
Schurr
Frank
F
Lallemand
Perrine
P
Ribière
Magali
M
D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics*; D005602:France; D014644:Genetic Variation; D007304:Insect Viruses / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
D004279:DNA, Viral