[Reactivation of hypermethylated GSTP1 promoter activity in MCF-7 cells by treatment with a component of natural drug].
OBJECTIVE
To investigate the reactivation of hypermethylated GSTP1 (glutathione S-transferase P1) promoter activity by a component of natural drug, CDP.


METHODS
The recombinant plasmid of pGL3-GSTP1 pro(m) containing hypermethylated GSTP1 gene promoter was constructed and then used to transiently transfect the human breast cancer cell line MCF-7 cell. After treatment with CDP and 5-aza-C, The luciferase activity in cell lysates were assayed.


RESULTS
Low promoter activities were found in hypermethylated GSTP1 promoter. The promoter activities were reactivated and in a CDP dose-dependent mode.


CONCLUSIONS
CDP has the ability to reactivate the hypermethylated GSTP1 gene promoter activity.
Zhang
Jun-zhe
JZ
Department of Biochemistry and molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Wang
Jin-hong
JH
Qin
Yang
Y
Fu
Qiang
Q
D001943:Breast Neoplasms / Q000235:genetics; D045744:Cell Line, Tumor; D019175:DNA Methylation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D006801:Humans; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D014162:Transfection
D004365:Drugs, Chinese Herbal; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi