Proteomic analysis of Tunisian grapevine cultivar Razegui under salt stress.
Salt stress is one of the major abiotic stresses in agriculture worldwide, especially in the Mediterranean area. We report here a proteomic approach to investigate the salt stress-responsive proteins in grapevine (Vitis vinifera). Two-dimensional electrophoresis (2-DE) was used to analyze the proteome of the salt-tolerant Tunisian grapevine cultivar Razegui, subjected to a supply of 100mm NaCl over 15d. Analysis of 2-DE gels derived from stressed plants revealed more than 800 reproducibly detected protein spots, with 48 proteins displaying a differential expression pattern, including 32 up-regulated, 9 down-regulated and 7 new protein spots induced after salt treatment. The presence of stress-responsive proteins in the different plant organs suggests that salt spreads systemically. Edman degradation analysis and database searching aided us in identifying a major protein GP. Database analysis revealed that this peptide has a 98% sequence similarity with a pathogenesis-related (PR) protein 10 (V. vinifera). A full-length cDNA encoding the GP protein was isolated from grapevine salt-stressed leaves and sequenced. The predicted protein contained 158 amino acids and showed 98% identity with PR10 protein of of V. vinifera (accession no. Cac16165).
Jellouli
Neila
N
CBBC, Laboratoire de physiologie Moléculaire de la Vigne, Centre de Biotechnologie de Borj Cedria. PO Box 901, Hammam-Lif 2050, Tunisia. jel_neil@yahoo.fr
Ben Jouira
Hatem
H
Skouri
Houda
H
Ghorbel
Abdelwahed
A
Gourgouri
Ali
A
Mliki
Ahmed
A
D001483:Base Sequence; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D040901:Proteomics / Q000379:methods; D016415:Sequence Alignment; D012965:Sodium Chloride / Q000494:pharmacology*; D027843:Vitis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
D010940:Plant Proteins; D020543:Proteome; D012965:Sodium Chloride