Sodium valproate does not augment Prpsc in murine neuroblastoma cells.
Sodium valproate (VPA) has been reported to increase the accumulation of the pathologic isoform of prion protein (PrPsc) in scrapie-infected murine neuroblastoma cells. In this study, the effect of VPA on PrPsc accumulation was investigated in murine N2a neuroblastoma cells chronically infected with scrapie strain 22L (N2a-22L). No accumulation of PrPsc was detected after short-term (3 days) or long-term (21 days) treatment of N2a-22L cells with 4.8, 12, 18 or 24 microM VPA. Higher VPA concentrations (240 and 600 microM) also failed to augment PrPsc expression. In conclusion, in our experimental conditions, no deleterious effect was induced by VPA on prions replication.
Legendre
C
C
Service de Neurovirologie, CEA, CRSSA, Université Paris XI, EPHE, IPSC, Fontenay-aux-Roses, France.
Casagrande
F
F
Andrieu
T
T
Dormont
D
D
Clayette
P
P
D000818:Animals; D045744:Cell Line, Tumor; D002746:Chlorpromazine / Q000494:pharmacology; D018492:Dopamine Antagonists / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism* / Q000382:microbiology; D011328:Prions / Q000378:metabolism*; D012608:Scrapie / Q000821:virology; D013997:Time Factors; D014635:Valproic Acid / Q000494:pharmacology*
D018492:Dopamine Antagonists; D004791:Enzyme Inhibitors; D011328:Prions; D014635:Valproic Acid; D002746:Chlorpromazine