title: The pentatricopeptide repeat gene OTP43 is required for trans-splicing of the mitochondrial nad1 Intron 1 in Arabidopsis thaliana.
abstract: The mitochondrial NADH:ubiquinone oxidoreductase complex (Complex I) is a large protein complex formed from both nuclearly and mitochondrially encoded subunits. Subunit ND1 is encoded by a mitochondrial gene comprising five exons, and the mature transcript requires four RNA splicing events, two of which involve trans-splicing independently transcribed RNAs. We have identified a nuclear gene (OTP43) absolutely required for trans-splicing of intron 1 (and only intron 1) of Arabidopsis thaliana nad1 transcripts. This gene encodes a previously uncharacterized pentatricopeptide repeat protein. Mutant Arabidopsis plants with a disrupted OTP43 gene do not present detectable mitochondrial Complex I activity and show severe defects in seed development, germination, and to a lesser extent in plant growth. The alternative respiratory pathway involving alternative oxidase is significantly induced in the mutant.
authors.affiliation: Unité Mixte de Recherche Génomique Végétale (Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Université d'Evry/Val d'Essone), 91057 Evry, France.
authors.forename: Andéol Falcon
authors.initials: AF
authors.lastname: de Longevialle
authors.forename: Etienne H
authors.initials: EH
authors.lastname: Meyer
authors.forename: Charles
authors.initials: C
authors.lastname: Andrés
authors.forename: Nicolas L
authors.initials: NL
authors.lastname: Taylor
authors.forename: Claire
authors.initials: C
authors.lastname: Lurin
authors.forename: A Harvey
authors.initials: AH
authors.lastname: Millar
authors.forename: Ian D
authors.initials: ID
authors.lastname: Small
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D020029:Base Pairing / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D042967:Electron Transport Complex I / Q000235:genetics* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D015151:Immunoblotting; D007438:Introns; D013058:Mass Spectrometry; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D008957:Models, Genetic; D009154:Mutation; D010641:Phenotype; D017393:RNA Editing / Q000235:genetics; D012326:RNA Splicing; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D024101:Mitochondrial Proteins; C036483:T-DNA; D042967:Electron Transport Complex I