[Determination of 16 quinolone residues in animal tissues using high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry].
A confirmative method was developed with high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-MS/MS) to simultaneously detect 16 quinolone residues in animal tissues, which included nalidixinic acid, oxolinic acid, flumequine, norfloxacin, enoxacin, ciprofloxacin, lomefloxacin, danofloxacin, enrofloxacin, ofloxacin, sarafloxacin, difloxacin, marbofloxacin, pefloxacin, sparfloxacin and orbifloxacin. In the method, the 16 residues were extracted with acidified acetonitrile, cleaned-up with hexane, and concentrated with a rotary evaporator. Then the reconstituted sample solution was analyzed using HPLC-MS/MS in positive mode, with a Inertsil C8-3 column as the analytical column. The method was validated at 10, 50 and 100 microg/kg. The validation results were as follows: the linear ranges were from 10 to 100 microg/kg; the overall recoveries were from 62.4% to 102% with the relative standard deviations of 1.4%-11.9%. The method is simple, rapid, and accurate, and its performance could meet the requirements of the domestic and international legislation. The method is applicable to simultaneously confirm multi-residues of quinolones in animal tissues such as chicken muscle, chicken liver and fish muscle.
Yue
Zhenfeng
Z
Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection & Quarantine Bureau, Shenzhen 518067, China. yuezhenfeng@163.com
Lin
Xiuyun
X
Tang
Shaobing
S
Chen
Xiaoxia
X
Ji
Caini
C
Hua
Honghui
H
Liu
Yu
Y
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000031:analogs & derivatives / Q000032:analysis; D015365:Enoxacin / Q000032:analysis; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000032:analysis; D009643:Norfloxacin / Q000032:analysis; D015242:Ofloxacin / Q000032:analysis; D015366:Pefloxacin / Q000032:analysis; D015363:Quinolones / Q000032:analysis*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D024841:Fluoroquinolones; D015363:Quinolones; C068581:danofloxacin; D015366:Pefloxacin; D015365:Enoxacin; D000077422:Enrofloxacin; D002939:Ciprofloxacin; C047225:difloxacin; C095163:orbifloxacin; C080260:marbofloxacin; D015242:Ofloxacin; C053091:lomefloxacin; D009643:Norfloxacin; C061363:sparfloxacin; C047224:sarafloxacin; C012976:flumequine