title: Effects of date seed oil on normal human skin in vitro.
abstract: Oxidative stress has been implicated in various skin diseases through the generation of reactive oxygen species and the depletion of endogenous antioxidant systems. The administration of antioxidants or free radical scavengers is reportedly helpful, notably in order to enhance the healing process. We investigated the protective effect of one new natural product:"date seed oil: DSO" against hydrogen peroxide (H(2)O(2))-induced oxidative stress, in terms of lipid peroxidation, depletion of endogenous antioxidant defense enzymes such as glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), one day after a 2 h exposure to H(2)O(2), using human skin organ culture as an in vitro model. In the investigated model system, DSO has significant protective effect, by inhibition of damage caused by H(2)O(2), endowing a radical scavenging ability. Treatment of skin with DSO inhibited H(2)O(2)-induced lipid peroxidation. In addition, this oil inhibited H(2)O(2)-induced depletion of antioxidant defense components, such as superoxide dismutase and catalase. We conclude that DSO could be useful in the attenuation of H(2)O(2)-induced oxidative stress-mediated skin diseases in human skin, possibly due to antioxidant properties.
authors.affiliation: Unité de Recherche, Pathologies Humaines et Stress Oxydatif, Institut Supérieur de Biotechnologie de Sfax, 3038, Sfax, Tunisia. inesdammak@yahoo.fr
authors.forename: Ines
authors.initials: I
authors.lastname: Dammak
authors.forename: Fatma
authors.initials: F
authors.lastname: Ben Abdallah
authors.forename: Sonia
authors.initials: S
authors.lastname: Boudaya
authors.forename: Laila
authors.initials: L
authors.lastname: Keskes
authors.forename: Souhail
authors.initials: S
authors.lastname: Besbes
authors.forename: Amel
authors.initials: A
authors.lastname: El Gaied
authors.forename: Hamadi
authors.initials: H
authors.lastname: Attia
authors.forename: Hamida
authors.initials: H
authors.lastname: Turki
authors.forename: Basma
authors.initials: B
authors.lastname: Hentati
mesh_terms: D000704:Analysis of Variance; D000975:Antioxidants / Q000494:pharmacology*; D028023:Arecaceae; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008315:Malondialdehyde / Q000378:metabolism; D009924:Organ Culture Techniques; D010938:Plant Oils / Q000494:pharmacology*; D012639:Seeds; D012867:Skin / Q000187:drug effects* / Q000201:enzymology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010938:Plant Oils; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase