title: Hemoglobin Ypsilanti: a high-oxygen-affinity hemoglobin demonstrated by two automated high-pressure liquid chromatography systems.
abstract: Hemoglobin (Hb) Ypsilanti is a rare high-oxygen-affinity hemoglobin. Like other high-oxygen-affinity hemoglobins, Hb Ypsilanti manifests as erythrocytosis. Because the migration of many high-oxygen-affinity variants on alkaline and acid gels does not differ from that of HbA, oxygen-hemoglobin dissociation studies are often used to document their presence. Hb Ypsilanti is a notable exception because its electrophoresis pattern on alkaline gel is highly characteristic, exemplifying the phenomenon of hybrid formation in variant hemoglobins. In the past few years, several laboratories have begun to use high-pressure liquid chromatography (HPLC) as a screen for hemoglobinopathies. We demonstrate the elution profile of Hb Ypsilanti on the 2 most widely used HPLC methods.
authors.affiliation: Department of Pathology, St Joseph Mercy Hospital and Warde Medical Laboratory, and Department of Pediatrics, University of Michigan Health System, Ann Arbor, MI 48106, USA.
authors.forename: Daniel D
authors.initials: DD
authors.lastname: Mais
authors.forename: Laurence A
authors.initials: LA
authors.lastname: Boxer
authors.forename: Ronald D
authors.initials: RD
authors.lastname: Gulbranson
authors.forename: David F
authors.initials: DF
authors.lastname: Keren
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D005192:Family Health; D005220:Fathers; D020022:Genetic Predisposition to Disease; D006455:Hemoglobins, Abnormal / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D007223:Infant; D008297:Male; D010100:Oxygen / Q000737:chemistry; D010108:Oxyhemoglobins / Q000378:metabolism*; D011086:Polycythemia / Q000097:blood / Q000175:diagnosis* / Q000235:genetics
chemical_list: D006455:Hemoglobins, Abnormal; D010108:Oxyhemoglobins; C070942:hemoglobin Ypsilanti; D010100:Oxygen