title: Bacillus subtilis AB1 controls Aeromonas infection in rainbow trout (Oncorhynchus mykiss, Walbaum).
abstract: OBJECTIVE
  To develop a probiotic with effectiveness against Aeromonas sp., which was pathogenic to rainbow trout.
  
  
  RESULTS
  When Bacillus subtilis AB1, which was obtained from fish intestine, was administered for 14 days to rainbow trout in feed at a concentration of 10(7) cells per gram either as viable, formalized or sonicated cells or as cell-free supernatant, the fish survived challenge with the pathogen. AB1 stimulated immune parameters, specifically stimulating respiratory burst, serum and gut lysozyme, peroxidase, phagocytic killing, total and alpha1-antiprotease and lymphocyte populations.
  
  
  CONCLUSIONS
  Bacillus subtilis AB1 was effective as a probiotic at controlling infections by a fish-pathogenic Aeromonas sp. in rainbow trout.
  
  
  CONCLUSIONS
  Disease control in fish is possible by means of the oral application of live and inactivated cells and their subcellular components with the mode of action reflecting stimulation of the innate immune response.
authors.affiliation: School of Life Sciences, Heriot-Watt University, Edinburgh, Scotland, UK.
authors.forename: A
authors.initials: A
authors.lastname: Newaj-Fyzul
authors.forename: A A
authors.initials: AA
authors.lastname: Adesiyun
authors.forename: A
authors.initials: A
authors.lastname: Mutani
authors.forename: A
authors.initials: A
authors.lastname: Ramsubhag
authors.forename: J
authors.initials: J
authors.lastname: Brunt
authors.forename: B
authors.initials: B
authors.lastname: Austin
mesh_terms: D000333:Aeromonas / Q000502:physiology*; D000818:Animals; D001412:Bacillus subtilis / Q000502:physiology*; D001770:Blood Bactericidal Activity; D003170:Complement Pathway, Alternative; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D005398:Fisheries; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D017686:Oncorhynchus mykiss / Q000097:blood / Q000276:immunology / Q000382:microbiology*; D019936:Probiotics; D000511:alpha-Macroglobulins / Q000032:analysis
chemical_list: D000511:alpha-Macroglobulins