Automated extended volume imaging of tissue using confocal and optical microscopy.
Conventional histologic techniques cannot readily be used for 3D reconstruction of large tissue volumes. We have developed an imaging rig which supports both confocal and light microscopy, and utilizes a surface imaging approach to serially image embedded tissue blocks while maintaining alignment and registration of the image series.
Sands
G B
GB
Bioengineering Institute, The University of Auckland, New Zealand. g.sands@auckland.ac.nz
Gerneke
D A
DA
Smaill
B H
BH
Le Grice
I J
IJ
D019412:Anatomy, Cross-Sectional / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D008867:Microtomy / Q000295:instrumentation* / Q000379:methods; D012371:Robotics / Q000295:instrumentation* / Q000379:methods; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods; D013382:Subtraction Technique / Q000295:instrumentation