title: Preparation and kinetic behavior of immobilized whole cell biocatalysts.
abstract: Actinoplanes missouriensis (for glucose isomerase), Kluyveromyces fragilis (for beta-galactosidase), and Saccharomyces cerevisiae (for invertase) cells were successfully entrapped within cellulose and cellulose di- and triacetate beads employing several carried solvent systems. Cellulose beads prepared using a melt of dimethylsulfoxide (DMSO) and N-ethylpyridinium chloride (NEPC), or cellulose diacetate using a mixture of acetone and DMSO as solvent, were found to be promising as carriers for the invertase system, cellulose triacetate beads with DMSO as solvent for yeast beta-galactosidase, and cellulose beads with a melt of DMSO and NEPC as solvent for glucose isomerase. The kinetic behavior of A. missouriensis glucose isomerase whole cell cellulose beads in a plug-flow column reactor was studied as an example system in greater detail.
authors.affiliation: Helsinki University of Technology, Department of Chemistry, SF-02150 Espoo 15, Finland.
authors.forename: P
authors.initials: P
authors.lastname: Linko
authors.forename: K
authors.initials: K
authors.lastname: Poutanen
authors.forename: L
authors.initials: L
authors.lastname: Weckstrom
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000737:chemistry; D001671:Biochemistry / Q000295:instrumentation* / Q000379:methods*; D002384:Catalysis; D018914:Cells, Immobilized; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D007700:Kinetics; D007716:Kluyveromyces / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: D012997:Solvents; D002482:Cellulose; C024671:cellulose triacetate; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase; D005947:Glucose