title: The effects of caffeic acid phenethyl ester (CAPE) on TNBS-induced colitis in ovariectomized rats.
abstract: OBJECTIVE
  The aim of this investigation was to examine the effects of caffeic acid phenethyl ester (CAPE) on the development of colitis and antioxidant parameters in bilateral ovariectomized rats subjected to trinitrobenzene sulfonic acid (TNBS)-induced colitis.
  
  
  METHODS
  Twenty-one Wistar Albino ovariectomized female rats were divided into four subgroups (n = 5 or 6) (colitis control, vehicle control, CAPE 10 and 30 mg/kg, respectively). Colitis was induced using an enema of TNBS and ethanol, following which CAPE was administrated for 3 days to induce colitis and effect of CAPE was subsequently evaluated.
  
  
  RESULTS
  Based on microscopic damage scores, there was no difference between rats of the TNBS-colitis and the vehicle-treated groups, whereas treatment with CAPE 10 and 30 mg/kg, respectively, caused a significant reduction in colon injury compared to that observed in rats of the TNBS-colitis and vehicle-treated groups. The histologies of both treatment groups were not significantly different. In terms of the biochemical analyses, myeloperoxidase levels in rats from the CAPE 10 and 30 mg/kg groups were significantly different from that of the colitis control rats; however, the levels of malondialdehyde (MDA), catalase and reduced glutathione (GSH) were only significantly different from the levels found colitis control rats in rats administered 10 mg/kg. The levels of MDA, GSH and SOD in rats given CAPE were also significantly different from those of rats in the vehicle control group. These results were consistent with histological findings.
  
  
  CONCLUSIONS
  CAPE may have a positive effect on the inflammatory bowel disease treatment process and could, therefore, be used as an adjunct therapy in colitis. These effects of CAPE may occur through antiinflammatory and antioxidant mechanisms.
authors.affiliation: Department of Physiology, Medical Faculty, Adnan Menderes University, Aydin 09100, Turkey. raufonur@yahoo.com
authors.forename: Rauf Onur
authors.initials: RO
authors.lastname: Ek
authors.forename: Mukadder
authors.initials: M
authors.lastname: Serter
authors.forename: Kemal
authors.initials: K
authors.lastname: Ergin
authors.forename: Yuksel
authors.initials: Y
authors.lastname: Yildiz
authors.forename: Serpil
authors.initials: S
authors.lastname: Cecen
authors.forename: Tulay
authors.initials: T
authors.lastname: Kavak
authors.forename: Cigdem
authors.initials: C
authors.lastname: Yenisey
mesh_terms: D000818:Animals; D002109:Caffeic Acids / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D003092:Colitis / Q000188:drug therapy* / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D010053:Ovary / Q000601:surgery; D009195:Peroxidase / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D002109:Caffeic Acids; D008315:Malondialdehyde; D014302:Trinitrobenzenesulfonic Acid; D002374:Catalase; D009195:Peroxidase; C055494:caffeic acid phenethyl ester; D005978:Glutathione; D010626:Phenylethyl Alcohol