[Study on effect of glycidyl methacrylate on cell cycle and apoptosis in human bronchial epithelial cells].
OBJECTIVE
To investigate the effect of glycidyl methacrylate (GMA) on cell cycle and apoptosis in human bronchial epithelial cells.


METHODS
Flow cytometry was applied to determine cell cycle and apoptosis of 16HBE cells treated at the doses of 1-16 microg/ml GMA for different time. Mitotic index (MI) of cell was also analysed.


RESULTS
When 16HBE cells were treated by GMA for 72hr, cells in G0/G1 phase decreased, but cells in S and G2/M phase increased. The increases of apoptosis rates and the decreases of mitotic index could also be observed. As treatment went on, all groups cells were stopped at G0/G1 phase, but at the dose of 16 microg/ml the cell increases of S and G2/M phase were observed in comparison with the controls. After cells were treated for 3 times, the decreases apoptosis rates and the increases of mitotic index of cell treated by high dosage were observed.


CONCLUSIONS
The 16HBE cells treated by GMA could result in cell cycle transition from G0/G1 phase to S and G2/M phase. It was suggested that the cell proliferation was stimulated. Uncontrolled cellular proliferation may be responsible for malignant transformation induced by GMA.
Yang
Min
M
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Wang
Quankai
Q
Wang
Yawen
Y
Li
Ying
Y
D000395:Air Pollutants, Occupational / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects*; D001980:Bronchi / Q000166:cytology*; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology*; D004852:Epoxy Compounds / Q000633:toxicity*; D005434:Flow Cytometry; D006801:Humans; D008689:Methacrylates / Q000633:toxicity*
D000395:Air Pollutants, Occupational; D004852:Epoxy Compounds; D008689:Methacrylates; C007870:glycidyl methacrylate