Dual transformation of homonuclear solid-state NMR spectra--an option to decrease measuring time.
Long measurement times due to low sensitivity are a prime concern in solid-state NMR and limit the application of multidimensional experiments severely. One possibility to address this problem could be post-experimental suppression of noise and a reduction of the number of increments needed for higher dimensional data sets. This can be achieved by a hybrid approach based on the combination of separately Fourier transformed and covariance processed datasets. The method is applied to synthetic sets as well as to experimental two-dimensional homonuclear solid-state NMR spectra of peptide samples. It is demonstrated that a reduction in experiment time by a factor of 4 can be achieved for the case of a 13C-13C correlation spectrum on the nonapeptide bradykinin.
Kaiser
Christoph
C
Institute for Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, J.W. Goethe University, Max von Laue Str. 7, 60438 Frankfurt am Main, Germany.
Lopez
Jakob J
JJ
Bermel
Wolfgang
W
Glaubitz
Clemens
C
D000465:Algorithms; D001920:Bradykinin / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008962:Models, Theoretical; D010455:Peptides / Q000737:chemistry*; D015203:Reproducibility of Results; D013997:Time Factors
D010455:Peptides; D001920:Bradykinin