title: Prognostic value of meiotic spindle imaging on fertilization rate and embryo development in in vitro-matured human oocytes.
abstract: OBJECTIVE
  To evaluate the relationship between spindle visualization and intracytoplasmic sperm injection (ICSI) outcomes in controlled ovarian stimulation (COS) cycles.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Assisted reproduction center.
  
  
  METHODS
  Thirty patients undergoing ICSI cycles.
  
  
  METHODS
  Meiotic spindle was evaluated before ICSI in 234 in vivo- and 101 in vitro-matured oocytes
  
  
  METHODS
  Meiotic spindle imaging, fertilization rate, and embryo development.
  
  
  RESULTS
  Spindle was present in 74.3% and 73.8% of the in vivo- and in vitro-matured oocytes, respectively. Spindle detection rate in oocytes derived from germinal vesicle and metaphase-I stage was, respectively, 50% and 86%. The fertilization rate achieved by the in vivo-matured oocytes was 71.8%, and spindle was detected in 75.6% of the fertilized oocytes and only 34.8.% of the nonfertilized oocytes. In the in vitro-matured oocytes, the fertilization rate was 66.1%, and spindle was detected in 81.4% of the fertilized oocytes and in 59.1% of the nonfertilized oocytes. Ten out of 43 (23.2%) in vitro-matured derived embryos were considered to be high quality, all derived from spindle-detected oocytes, which represents an increase of 13.0% on the overall number of high-quality embryos.
  
  
  CONCLUSIONS
  Meiotic spindle imaging may be useful to predict in vitro-matured oocyte development. However, other factors may contribute to the decreased developmental competence of in vitro-matured oocytes.
authors.affiliation: Fertility Assisted Fertilization Center, São Paulo, Brazil.
authors.forename: Daniela Paes de Almeida Ferreira
authors.initials: DP
authors.lastname: Braga
authors.forename: Rita de Cássia Sávio
authors.initials: Rde C
authors.lastname: Figueira
authors.forename: Débora
authors.initials: D
authors.lastname: Rodrigues
authors.forename: Camila
authors.initials: C
authors.lastname: Madaschi
authors.forename: Fabio Firmbach
authors.initials: FF
authors.lastname: Pasqualotto
authors.forename: Assumpto
authors.initials: A
authors.lastname: Iaconelli
authors.forename: Edson
authors.initials: E
authors.lastname: Borges
mesh_terms: D000328:Adult; D047108:Embryonic Development; D005260:Female; D005306:Fertilization / Q000502:physiology; D005307:Fertilization in Vitro; D006801:Humans; D054315:Oocyte Retrieval; D009865:Oocytes / Q000648:ultrastructure*; D011247:Pregnancy; D011379:Prognosis; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic; D008941:Spindle Apparatus / Q000648:ultrastructure*