title: [Anti-tumor constituents from Paris polyphylla var. yunnanensis].
abstract: OBJECTIVE
  To study the anti-tumor active constituents from the rhizome of Paris polyphylla var. yunnanensis.
  
  
  METHODS
  The compounds were isolated by column chromatography with silica gel, and purified by Sephadex LH-20 columu chromafography and reverse-phase preparative, HPLC. The structures were identified by spectroscopic methods. The anti-tumor experiment in vitro, the MTT, was used to screen constituents of P. polyphalla var. yunnanensis.
  
  
  RESULTS
  Six compounds were obtained and their structures were identified as diosgenin-3-O-alpha-L-arabinofuranosyl (1-->4) -beta-D-glycopyranoside (1), pennogenin-3-O-alpha-L-arabino-furanosyl (1-->4)-beta-D-glycopyranoside (2), isorhamn etin-3-O-beta-D-glycopyranoside (3), ethyl-alpha-D-fructofuranoside (4), pennogenin-3-O-alpha-L-rhamnopyranosyl (1-->4)-[alpha-L-rhamnopyranosyl (1-->2)]-beta-D-glycopyranoside (5) and pennogenin-3-O-alpha-L-rhamnopyranosyl (1-->4)-alpha-L-rhamnopyranosyl (1-->4)-[alpha-L-rhamnopyranosyl (1-->2)]-beta-D-glycopyranoside (6).
  
  
  CONCLUSIONS
  Compounds 1-4 were isolated from this plant for the first time, compounds 3 and 4 was firstly isolated from genus Paris, compound 5 was firstly isolated from the rhizome of this plant. The pharmacological results showed that compounds 1-3, 5 and 6 showed certain inhibition, especially, the activities of compound 5 and 6 were the most significant.
authors.affiliation: College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin 300072, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Yan-jun
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Wen-yuan
authors.initials: WY
authors.lastname: Gao
authors.forename: Lu-lu
authors.initials: LL
authors.lastname: Yan
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D019668:Liliaceae / Q000737:chemistry*; D008175:Lung Neoplasms / Q000473:pathology; D051379:Mice; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006027:Glycosides; D012503:Saponins; C525421:pennogenin-3-O-alpha-L-arabino-furanosyl (1-4)-beta-D-glycopyranoside; C033475:ethyl alpha-D-fructofuranoside