Detection of functional ligand-binding events using synchrotron x-ray scattering.
Small-molecule ligands that change the structure of a protein are likely to affect its function, whereas those causing no structural change are less likely to be functional. Wide-angle x-ray scattering (WAXS) can be easily carried out on proteins and small molecules in solution in the absence of chemical tags or derivatives. The authors demonstrate that WAXS is a sensitive probe of ligand binding to proteins in solution and can distinguish between nonfunctional and productive binding. Furthermore, similar ligand-binding modes translate into similar scattering patterns. This approach has high potential as a novel, generic, low-throughput assay for functional ligand binding.
Rodi
Diane J
DJ
Biosciences Division, Argonne National Laboratory, Argonne, Illinois 60439,USA. drodi@anl.gov
Mandava
Suneeta
S
Gore
David B
DB
Makowski
Lee
L
Fischetti
Robert F
RF
D008024:Ligands; D012542:Scattering, Radiation; D017356:Synchrotrons
D008024:Ligands