Microbiological assay for ceftazidime injection.
A simple, sensitive, and specific biodiffusion assay for the antibacterial ceftazidime was developed using a strain of Staphylococcus epidermidis (ATCC 12228) as the test organism. Ceftazidime was measured in powder for injection at concentrations ranging from 100 to 400 microg/mL. The calibration graph for ceftazidime was linear (r2 = 1), and the method validation showed that it was precise (relative standard deviation = 0.415) and accurate. The results obtained by biodiffusion assay were statistically calculated by linear parallel model and by means of regression analysis and were verified using analysis of variance. It was concluded that the microbiological assay is satisfactory for in vitro quantification of the antibacterial activity of ceftazidime in pharmaceuticals.
De Haro Moreno
Andréia
A
Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas, UNESP, Rodovia Araraquara-Jaú, km 1, CEP 14801-302, Araraquara-SP, Brazil.
Salgado
Hérida Regina Nunes
HR
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002442:Ceftazidime / Q000032:analysis*; D004058:Diffusion; D008826:Microbial Sensitivity Tests / Q000295:instrumentation / Q000379:methods*; D008956:Models, Chemical; D004364:Pharmaceutical Preparations / Q000737:chemistry; D011208:Powders; D012015:Reference Standards; D012044:Regression Analysis; D015203:Reproducibility of Results; D013212:Staphylococcus epidermidis / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D004364:Pharmaceutical Preparations; D011208:Powders; D002442:Ceftazidime