Isolation of CD24(high) and CD24(low/-) cells from MCF-7: CD24 expression is positively related with proliferation, adhesion and invasion in MCF-7.
CD24 has been reported to have a role in metastasis of several malignancies including breast cancer. We investigated the effect of CD24 expression on biologic features in human breast cancer cells, and found that CD24 expression is associated with more rapid growth and enhanced ability of adhesion and invasion in MCF-7. The proportion of cells was higher in synthetic phase and lower in arrestic phase for CD24(high) than for CD24(low/-) cells. Cyclin D1 and p27 had stronger expressions in CD24(low/-) cells than CD24(high) cells. These suggested one possible pathway for CD24 effect on proliferation.
Kim
Hee Joung
HJ
Department of Surgery, Seoul National University College of Medicine, 28 Youngon-Dong, Chongon-Gu, Seoul, Republic of Korea. nirvana804@hanmail.net
Kim
Jong Bin
JB
Lee
Kyung-Min
KM
Shin
Incheol
I
Han
Wonshik
W
Ko
Eunyoung
E
Bae
Ji-Yeon
JY
Noh
Dong-Young
DY
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051927:CD24 Antigen / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D056741:Cyclin D; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D009361:Neoplasm Invasiveness / Q000473:pathology; D013629:Tamoxifen / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D000903:Antibiotics, Antineoplastic; D018931:Antineoplastic Agents, Hormonal; D051927:CD24 Antigen; D056741:Cyclin D; D016213:Cyclins; D047628:Estrogen Receptor alpha; D013629:Tamoxifen; D050760:Cyclin-Dependent Kinase Inhibitor p27; D004317:Doxorubicin