title: Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) Protein (Na-ASP1).
abstract: Human hookworm infection is one of the most significant parasitic infections, and a leading global cause of anemia and malnutrition of adults and children in rural areas of the tropics and subtropics. Necator americanus secretory protein (Na-ASP1), which is a potential vaccine candidate against hookworm infections, has been expressed in Pichia pastoris. Na-ASP1 protein was expressed extracellulary by employing the leader sequence of the alpha-mating factor of Saccharomyces cerevisiae. Most of the protein produced by single copy clones was secreted outside the cell. The Na-ASP1 steady state mRNA levels of the clones were correlated to their Na-ASP1 gene copy number. However, increasing gene copy number of Na-ASP1 protein in P. pastoris saturated secretory capacity and therefore, decreased the amount of secreted protein in clones harboring multiple copies of Na-ASP1 gene.
authors.affiliation: Department of Chemical Engineering, University of Nebraska, Lincoln, NE. USA.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Inan
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Fanders
authors.forename: Wenhui
authors.initials: W
authors.lastname: Zhang
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Hotez
authors.forename: Bin
authors.initials: B
authors.lastname: Zhan
authors.forename: Michael M
authors.initials: MM
authors.lastname: Meagher
mesh_terms: D000723:Ancylostomatoidea / Q000378:metabolism*; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002999:Clone Cells; D003062:Codon; D018628:Gene Dosage; D015870:Gene Expression; D005822:Genetic Vectors; D015801:Helminth Proteins / Q000235:genetics*; D010843:Pichia / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D003062:Codon; D015801:Helminth Proteins