title: Dense-granule protein NcGRA7, a new marker for the serodiagnosis of Neospora caninum infection in aborting cows.
abstract: To investigate whether the production of an antigen-specific antibody is associated with Neospora caninum-induced bovine abortion, 62 serum samples were tested with an enzyme-linked immunosorbent assay using the recombinant antigens NcSAG1, NcSRS2, and NcGRA7. Our study suggested that NcGRA7 would be a new marker for the serodiagnosis of N. caninum infection resulting in abortion.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Penglong
authors.initials: P
authors.lastname: Huang
authors.forename: Min
authors.initials: M
authors.lastname: Liao
authors.forename: Houshuang
authors.initials: H
authors.lastname: Zhang
authors.forename: Eung-Goo
authors.initials: EG
authors.lastname: Lee
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
mesh_terms: D000034:Abortion, Veterinary / Q000469:parasitology*; D000818:Animals; D000906:Antibodies / Q000097:blood* / Q000276:immunology; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005982:Glutathione Transferase / Q000378:metabolism; D018688:Neospora / Q000302:isolation & purification*; D011247:Pregnancy; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism
chemical_list: D000906:Antibodies; D000953:Antigens, Protozoan; D015415:Biomarkers; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase