Non-viral charge reversal vectors for pDNA delivery.
A synthetic vector that transform from a cationic to an anionic lipid intracellularly is described. This charge-reversal lipid was synthesized and characterized, including the supramolecular complex it forms with DNA. Enhanced gene transfection was observed using this synthetic vector compared to current cationic lipids.
Prata
Carla A H
CA
Dept. of Biomed. Eng., Boston Univ., MA 02215, USA. cprata@bu.edu
Li
Yougen
Y
Luo
Dan
D
McIntosh
Thomas J
TJ
Grinstaff
Mark W
MW
D000818:Animals; D000838:Anions / Q000737:chemistry*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000737:chemistry*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D020014:K562 Cells; D008027:Light; D008055:Lipids / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D012542:Scattering, Radiation; D014961:X-Ray Diffraction
D000838:Anions; D008055:Lipids; D004247:DNA