Dentin demineralization when subjected to BioPure MTAD: a longitudinal and quantitative assessment.
In the present study, the demineralizing ability of BioPure MTAD (Dentsply/Tulsa, Tulsa, OK), 17% EDTA, and 5% citric acid on radicular dentin was quantitatively and longitudinally analyzed. 3 mm thick disks were obtained at the root cervical third from 9 maxillary human molars, and a standardized smear layer was produced. Cosite images of the dentin surface were obtained after several cumulative demineralization times. Sixteen images were obtained in each dentin sample for each experimental time, at 1000x magnification. An image processing and analysis sequence measured sets of images, providing data of area fraction for thousands of tubules over time. Thus, it was possible to follow the demineralization phenomenon and quantitatively analyze the effect of the various substances. The nonparametric Kruskal-Wallis H-test was used to analyze the data. Based on the present results, it can be concluded that the demineralization kinetics promoted by both 5% CA and BioPure MTAD was significantly faster than by 17% EDTA.
De-Deus
Gustavo
G
Veiga de Almeida University, Rio de Janeiro, Brazil.
Reis
Claudia
C
Fidel
Sandra
S
Fidel
Rivail
R
Paciornik
Sidnei
S
D002614:Chelating Agents / Q000494:pharmacology*; D019343:Citric Acid / Q000494:pharmacology*; D003648:Decalcification Technique; D003804:Dentin / Q000737:chemistry* / Q000187:drug effects* / Q000648:ultrastructure; D003806:Dentin Permeability; D004318:Doxycycline / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008963:Molar; D011136:Polysorbates / Q000494:pharmacology*; D012388:Root Canal Irrigants / Q000494:pharmacology*; D016369:Smear Layer; D018709:Statistics, Nonparametric; D013997:Time Factors
D002614:Chelating Agents; C475043:MTAD (intracanal irrigant); D011136:Polysorbates; D012388:Root Canal Irrigants; D019343:Citric Acid; D004492:Edetic Acid; D004318:Doxycycline