[Association between gamma-H2AX and DNA double-strand breaks].
H2AX may be one family of histone and distributes among the genome. It is reported that DNA double-strand breaks (DSB) could induce phosphorylation of H2AX at conservative C-terminal region of serine 139 and the formation of gamma-H2AX. The gamma-H2AX foci can be observed by the fluorescence microscope. An immunofluorescence assay with specific antibodies recognizing gamma-H2AX could become the gold standard for detecting the DSB. It was indicated that some of physical, chemical, and biological factors could induce the formation of the gamma-H2AX foci. In this paper, the advance of the studies of mechanism of gamma-H2AX formation and the association between gamma-H2AX and DSB were reviewed.
Bin
Ping
P
National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Zheng
Yuxin
Y
D053903:DNA Breaks, Double-Stranded / Q000187:drug effects / Q000528:radiation effects; D006657:Histones / Q000378:metabolism*; D006801:Humans; D010750:Phosphoproteins / Q000378:metabolism*
C468783:H2AX protein, human; D006657:Histones; D010750:Phosphoproteins