Assessment of collagenase activity in cartilage.
Assay of collagenase activity involves the use of radiolabeled collagen. Stimulation of cartilage with proinflammatory cytokines results in the upregulation of collagenases and the subsequent release of degraded collagen fragments. These enzymes can be localized in both osteoarthritic and rheumatoid arthritis cartilage and synovial tissues.
Cawston
Tim E
TE
Morgan
Tanya G
TG
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000201:enzymology; D002356:Cartilage / Q000201:enzymology*; D002417:Cattle; D024042:Collagen Type I; D017364:Collagenases / Q000032:analysis* / Q000378:metabolism; D017077:Culture Media, Conditioned; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D020784:Matrix Metalloproteinase 8 / Q000378:metabolism; D010003:Osteoarthritis / Q000201:enzymology; D013379:Substrate Specificity; D013583:Synovial Membrane / Q000201:enzymology; D046509:Tissue Culture Techniques; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D014316:Tritium
D024042:Collagen Type I; D017077:Culture Media, Conditioned; D019715:Tissue Inhibitor of Metalloproteinase-1; D014316:Tritium; D017364:Collagenases; D053509:Matrix Metalloproteinase 13; D020784:Matrix Metalloproteinase 8; D020781:Matrix Metalloproteinase 1