title: [Reactivation of hypermethylated GSTP1 promoter activity in MCF-7 cells by treatment with a component of natural drug].
abstract: OBJECTIVE
  To investigate the reactivation of hypermethylated GSTP1 (glutathione S-transferase P1) promoter activity by a component of natural drug, CDP.
  
  
  METHODS
  The recombinant plasmid of pGL3-GSTP1 pro(m) containing hypermethylated GSTP1 gene promoter was constructed and then used to transiently transfect the human breast cancer cell line MCF-7 cell. After treatment with CDP and 5-aza-C, The luciferase activity in cell lysates were assayed.
  
  
  RESULTS
  Low promoter activities were found in hypermethylated GSTP1 promoter. The promoter activities were reactivated and in a CDP dose-dependent mode.
  
  
  CONCLUSIONS
  CDP has the ability to reactivate the hypermethylated GSTP1 gene promoter activity.
authors.affiliation: Department of Biochemistry and molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Jun-zhe
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Jin-hong
authors.initials: JH
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Qin
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics; D045744:Cell Line, Tumor; D019175:DNA Methylation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D006801:Humans; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D014162:Transfection
chemical_list: D004365:Drugs, Chinese Herbal; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi