Detection of DNA double-strand breaks using gammaH2AX after MRI exposure at 3 Tesla: an in vitro study.
OBJECTIVE
To evaluate the effects of the static magnetic field and typical imaging sequences of a high-field magnetic resonance scanner (3 Tesla) on the induction of double-strand breaks (DSBs) in two different human cell lines.


METHODS
Human promyelocytic leukemia cells (HL-60) and human acute myeloid leukemia cells (KG-1a) were exposed to the static magnetic field alone and to turbo spin-echo (TSE) and gradient-echo (GE) sequences. Flow cytometry was used to quantify gammaH2AX (serine 139 phosphorylated form of histone H2AX) expression of antibody-stained cells as a marker for deoxyribonucleic acid (DNA) DSBs one hour and 24 hours after magnetic field exposure. X-ray-treated cells were used as positive control.


RESULTS
Neither exposure to the static magnetic field alone nor to the applied imaging sequences showed significant differences in gammaH2AX expression between exposed and sham-exposed cells. X-ray-treated cells as positive control showed a significant increase in gammaH2AX expression.


CONCLUSIONS
The static magnetic field alone and MRI sequences at 3 Tesla have no effect on the induction of DSBs in HL-60 and KG-1a cells.
Schwenzer
Nina F
NF
Department of Diagnostic Radiology, Eberhard-Karls University, Tübingen, Germany. nina.schwenzer@med.uni-tuebingen.de
Bantleon
Rüdiger
R
Maurer
Brigitte
B
Kehlbach
Rainer
R
Schraml
Christina
C
Claussen
Claus D
CD
Rodegerdts
Enno
E
D002460:Cell Line; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000235:genetics* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D018922:HL-60 Cells; D006657:Histones / Q000235:genetics* / Q000528:radiation effects*; D006801:Humans; D008279:Magnetic Resonance Imaging; D011829:Radiation Dosage
D004273:DNA, Neoplasm; C468783:H2AX protein, human; D006657:Histones