title: Necdin mediates skeletal muscle regeneration by promoting myoblast survival and differentiation.
abstract: Regeneration of muscle fibers that are lost during pathological muscle degeneration or after injuries is sustained by the production of new myofibers. An important cell type involved in muscle regeneration is the satellite cell. Necdin is a protein expressed in satellite cell-derived myogenic precursors during perinatal growth. However, its function in myogenesis is not known. We compare transgenic mice that overexpress necdin in skeletal muscle with both wild-type and necdin null mice. After muscle injury the necdin null mice show a considerable defect in muscle healing, whereas mice that overexpress necdin show a substantial increase in myofiber regeneration. We also find that in muscle, necdin increases myogenin expression, accelerates differentiation, and counteracts myoblast apoptosis. Collectively, these data clarify the function and mechanism of necdin in skeletal muscle and show the importance of necdin in muscle regeneration.
authors.affiliation: Department of Histology and Medical Embryology, University of Roma-La Sapienza, 00161 Rome, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Deponti
authors.forename: Stéphanie
authors.initials: S
authors.lastname: François
authors.forename: Silvia
authors.initials: S
authors.lastname: Baesso
authors.forename: Clara
authors.initials: C
authors.lastname: Sciorati
authors.forename: Anna
authors.initials: A
authors.lastname: Innocenzi
authors.forename: Vania
authors.initials: V
authors.lastname: Broccoli
authors.forename: Françoise
authors.initials: F
authors.lastname: Muscatelli
authors.forename: Raffaella
authors.initials: R
authors.lastname: Meneveri
authors.forename: Emilio
authors.initials: E
authors.lastname: Clementi
authors.forename: Giulio
authors.initials: G
authors.lastname: Cossu
authors.forename: Silvia
authors.initials: S
authors.lastname: Brunelli
mesh_terms: D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D002459:Cell Fusion; D002470:Cell Survival; D002478:Cells, Cultured; D051379:Mice; D008822:Mice, Transgenic; D018485:Muscle Fibers, Skeletal / Q000166:cytology; D018482:Muscle, Skeletal / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D017570:MyoD Protein / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology*; D018008:Myogenin / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000378:metabolism*; D009687:Nuclear Proteins / Q000172:deficiency / Q000378:metabolism*; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology; D015533:Transcriptional Activation
chemical_list: D017570:MyoD Protein; D018008:Myogenin; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; C069766:necdin