Rat nerve regeneration with the use of a polymeric membrane loaded with NGF.
Exogenous neurotrophic factors, delivered by various systems, are used to improve nerve regeneration. This study tested the effectiveness of a polymeric membrane loaded with Nerve Growth Factor (NGF) on mental nerve regeneration after a crush injury in rats. We tested NGF application, known to play a role in afferent fiber repair in dental neurobiology, to see if it could improve the regeneration. Afferent neurogram recordings and histological analyses of the trigeminal ganglion neurons were performed. One month after the crush injury, early regeneration was observed independently of exogenous NGF. However, as compared with the activity level recorded before the injury, the afferent activity was reduced by 28.5% without NGF, and the mean number of labeled neurons decreased. With NGF, activity was increased by 30.8%, with no significant histological difference compared with animals without lesions. NGF application through a polymeric membrane can influence degenerative and/or regenerative processes after a crush injury.
Savignat
M
M
Faculté de Chirurgie Dentaire, Université de Lille 2, Place de Verdun, 59000 Lille, France. mathilde.savignat@univ-lille2.fr
De-Doncker
L
L
Vodouhe
C
C
Garza
J M
JM
Lavalle
P
P
Libersa
P
P
D000818:Animals; D019344:Lactic Acid; D008297:Male; D008340:Mandibular Nerve / Q000187:drug effects / Q000502:physiology; D008567:Membranes, Artificial; D009409:Nerve Crush; D020932:Nerve Growth Factor / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects*; D009475:Neurons, Afferent / Q000187:drug effects; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D051381:Rats; D017208:Rats, Wistar; D061221:Trigeminal Nerve Injuries
D008567:Membranes, Artificial; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D020932:Nerve Growth Factor