Tint maps to mouse chromosome 6 and may interact with a notochordal enhancer of Brachyury.
At the proximal part of mouse chromosome 17 there are three well-defined genes affecting the axis of the embryo and consequently tail length: Brachyury, Brachyury the second, and the t-complex tail interaction (T1, T2, and tct). The existence of T1 and tct in fact defines the classical "t-complex" that occupies approximately 40 cM of mouse chromosome 17. Their relationship to each other and various unlinked interacting genes has been enigmatic. The tint gene was the first of the latter to be identified. We report here its genetic mapping using a microsatellite scan together with outcrosses to Mus spretus and M. castaneous followed by a subsequent testcross to T, T1, and T2 mutants. Surprisingly, tint interacts with T2 but not with T1. The implications of our data suggest that T2 may be part of the T1 regulatory region through direct or indirect participation of tint.
Wu
Jiang I
JI
Institute for Cellular and Molecular Biology, Section of Molecular Genetics and Microbiology, University of Texas, Austin, Texas 78712-1064, USA.
Centilli
M A
MA
Vasquez
Gabriela
G
Young
Susan
S
Scolnick
Jonathan
J
Durfee
Larissa A
LA
Spearow
Jimmy L
JL
Schwantz
Staci D
SD
Rennebeck
Gabriela
G
Artzt
Karen
K
D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes; D004742:Enhancer Elements, Genetic; D005326:Fetal Proteins / Q000235:genetics*; D051379:Mice; D009672:Notochord; D020825:T-Box Domain Proteins / Q000235:genetics*; D000098908:Brachyury Protein
D005326:Fetal Proteins; D020825:T-Box Domain Proteins; D000098908:Brachyury Protein