Antimutagenic potential of the Thai herb, Mucuna collettii Lace.
Mucuna collettii Lace is a Thai herb with a long record of consumption among mature Thai males for the promotion of sexual potency. The mutagenic and antimutagenic potentials of Mucuna collettii extract were carried out by using the Ames test pre-incubation method in the presence and absence of S9 mixture. Salmonella typhimurium strains TA 98 and TA 100 were applied as the tester strains. Prior to mutagenic and antimutagenic tests, the survival of the tester strains was performed by treating with the plant extract. Results showed Mucuna collettii extract exhibited strong cytotoxic effects in a dose-dependent manner. Toxicity of the plant was confirmed in mice in which negative adverse effect was found in kidney, uterus, ovary, and testis. Mucuna collettii extract in the presence and absence of S9 mixture was negative for mutagenic Ames test. Mucuna collettii extract in the presence and absence of S9 mixture was positive for antimutagenic Ames test towards either one or both of the tested mutagens: 2-(2-furyl)-3-(5-nitro-2-furyl)-acrylamide (AF-2) and benzo(a)pyrene. The antimutagenic activity of the plant extract was confirmed in rec-assays. Micronucleus test demonstrated that Mucuna collettii extract at high dose and a long incubation time could induce micronucleus formation in tested animals, but less than the response of the positive control. The overall mutagenic and antimutagenic assays are further evidences for the antimutagenic potential of Mucuna collettii.
Cherdshewasart
Wichai
W
Department of Biology, Faculty of Science, Chulalongkorn University, Phyathai Road, Patumwan, Bangkok 10330, Thailand. cwichai@sc.chula.ac.th
Sutjit
Wandee
W
Pulcharoen
Kade
K
Panriansaen
Rattana
R
Chulasiri
Malyn
M
D000818:Animals; D016587:Antimutagenic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D001564:Benzo(a)pyrene / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005260:Female; D005668:Furylfuramide / Q000633:toxicity; D008297:Male; D008518:Medicine, East Asian Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D031299:Mucuna / Q000737:chemistry*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013785:Thailand; D036401:Toxicity Tests, Chronic
D016587:Antimutagenic Agents; D009153:Mutagens; D010936:Plant Extracts; D005668:Furylfuramide; D001564:Benzo(a)pyrene