title: Quantification of CD10 mRNA in colorectal cancer and relationship between mRNA expression and liver metastasis.
abstract: CD10 mRNA expression in colorectal cancer and its relationship with cancer progression and prognosis were investigated.
  
  
  METHODS
  CD10 mRNA was quantified in 167 colorectal cancer and matched normal tissue samples using real-time polymerase chain reaction (RT-PCR). The tumor to normal tissue (TIN) CD10 mRNA ratio was compared with clinicopathological factors and prognosis.
  
  
  RESULTS
  CD10 mRNA was overexpressed in 138 of the 167 tumors in comparison with the matched normal tissues. TIN was higher in colon, pN1/pN2, stage III and IV, and well- or moderately-differentiated adenocarcinoma than in rectum, pNO, stage I and II, and poorly-differentiated or mucinous adenocarcinoma, respectively. However, these differences were not significant. TIN was not associated with prognosis.
  
  
  CONCLUSIONS
  CD10 mRNA showed significantly higher expression in tumor tissues than in matched normal tissues. Although CD10 mRNA was associated with invasion depth, lymph node status and TNM stage, it was not associated with prognosis.
authors.affiliation: Department of Surgery, National Cancer Center Hospital, Tokyo, Japan. sfujita@ncc.go.jp
authors.forename: Shin
authors.initials: S
authors.lastname: Fujita
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Akasu
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Moriya
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Taniguchi
authors.forename: Tadakazu
authors.initials: T
authors.lastname: Shimoda
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000556:secondary; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology*; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000276:immunology / Q000556:secondary*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D015260:Neprilysin / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D015260:Neprilysin