title: Effect of exercise duration on ceramide metabolism in the rat heart.
abstract: OBJECTIVE
  We aimed at gaining more insight into the mechanisms underlying exercise-induced alterations in myocardial ceramide (CER) content by employing physical activity of various durations and examining all key pathways of CER metabolism.
  
  
  METHODS
  The experiments were carried out on male Wistar rats divided into four groups (n = 6 in each case): control, exercised for 30 and 90 min and until exhaustion on the electrically driven treadmill moving with a speed of 1200 m h(-1) and set at +10 degrees incline. The animals were anaesthetized and samples of the heart's left ventricle were excised.
  
  
  RESULTS
  Thirty-minute exercise decreased the level of CER in the heart by 15%. However, after 90 min of running it returned to the baseline and at the point of exhaustion it exceeded that of the control animals by 26%. The initial reduction in the content of CER was probably a result of its augmented degradation, as a concomitant elevation in the activity of acid ceramidase and the level of sphingosine was observed. The transition from reduction in CER content after 30 min of exercise to its accumulation at the point of exhaustion was a consequence of gradual reduction in the activity of acid ceramidase and simultaneous increase in the rate of de novo CER synthesis, as evidenced by progressive activation of serine palmitoyltransferase and accumulation of sphinganine.
  
  
  CONCLUSIONS
  We conclude that the effect of physical effort on myocardial CER content and metabolism depends to a large extent on exercise duration.
authors.affiliation: Department of Physiology, Medical University of Bialystok, Bialystok, Poland. marcinb@amb.edu.pl
authors.forename: M
authors.initials: M
authors.lastname: Baranowski
authors.forename: P
authors.initials: P
authors.lastname: Zabielski
authors.forename: A
authors.initials: A
authors.lastname: Blachnio
authors.forename: J
authors.initials: J
authors.lastname: Gorski
mesh_terms: D055573:Acid Ceramidase; D000214:Acyl Coenzyme A / Q000032:analysis; D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D055572:Ceramidases; D002518:Ceramides / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D006321:Heart; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism; D013997:Time Factors
chemical_list: D000214:Acyl Coenzyme A; D002518:Ceramides; D005230:Fatty Acids, Nonesterified; D013108:Sphingomyelin Phosphodiesterase; D000581:Amidohydrolases; D055573:Acid Ceramidase; C526736:Asah1 protein, rat; D055572:Ceramidases