title: Effect of thalidomide on endostatin levels in retinal ischemia/reperfusion injury.
abstract: OBJECTIVE
  To evaluate the effects of thalidomide treatment on endostatin production in ischemia/reperfusion-injured guinea pigs retinas.
  
  
  METHODS
  Control, ischemia, thalidomide/ischemia groups including 7 animals in each were formed from pigmented male guinea pigs. Retinal ischemia was induced by cannulating anterior chambers and lifting the bottle to a height of 205 cm for 90 min in the ischemia and thalidomide/ischemia groups. The thalidomide/ischemia group received thalidomide (300 mg/kg/day) via nasogastric tube 24 h before ischemia and during 7 days of reperfusion. Guinea pigs were sacrified for biochemical analysis of retinal endostatin level by ELISA.
  
  
  RESULTS
  The mean retinal endostatin levels of the control, ischemia and thalidomide/ischemia groups were 11.79 +/- 7.36, 14.70 +/- 4.88, 30.94 +/- 15.59 ng/ml, respectively. The mean retinal endostatin level of the thalidomide/ischemia group was significantly higher than that of the control group (p = 0.011). The mean retinal endostatin level of the thalidomide/ischemia group was higher than that of the ischemia group, but there was no statistically significant difference (p = 0.073). No statistically significant difference in the mean retinal endostatin level was found between the control and ischemia groups (p = 0.165).
  
  
  CONCLUSIONS
  Thalidomide treatment increased retinal endostatin level in ischemia/reperfusion-injured guinea pig retinas.
authors.affiliation: Department of Ophthalmology, Firat University School of Medicine, Elaziğ, Turkey.
authors.forename: Semih
authors.initials: S
authors.lastname: Aydoğan
authors.forename: Peykan
authors.initials: P
authors.lastname: Türkçüoğlu
authors.forename: Ulkü
authors.initials: U
authors.lastname: Celiker
authors.forename: Nevin
authors.initials: N
authors.lastname: Ilhan
mesh_terms: D000818:Animals; D043169:Endostatins / Q000378:metabolism*; D006168:Guinea Pigs; D007441:Intubation, Gastrointestinal; D008297:Male; D015427:Reperfusion Injury / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*; D012171:Retinal Vessels; D013792:Thalidomide / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D043169:Endostatins; D013792:Thalidomide