Characterization of nuclear localization and nuclear export signals of yeast actin-binding protein Pan1.
Pan1 is an actin patch-associated protein involved in endocytosis. Our studies revealed that in oleate-grown cells Pan1 is located in the nucleus as well as in patches. One of three putative nuclear localization signals (NLS) of Pan1, NLS2, directed beta-galactosidase (beta-gal) to the nucleus. However, GFP-Pan1(886-1219), containing NLS2, was found in the cytoplasm indicating that it may contain a nuclear export signal (NES). A putative Pan1 NES, overlapping with NLS3, re-addressed NLS(H2B)-NES/NLS3-beta-gal from the nucleus to the cytoplasm. Inactivation of the NES allowed NLS3 to be effective. Thus, Pan1 contains functional NLSs and a NES and appears to shuttle in certain circumstances.
Kamińska
Joanna
J
Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 01-106 Warsaw, Poland. kaminska@ibb.waw.pl
Sedek
Magdalena
M
Wysocka-Kapcińska
Monika
M
Zoładek
Teresa
T
D021581:Active Transport, Cell Nucleus; D002467:Cell Nucleus / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter / Q000235:genetics; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D049790:Nuclear Export Signals; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins
D005656:Fungal Proteins; D008840:Microfilament Proteins; D049790:Nuclear Export Signals; C111870:PAN1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins