[Evaluation of an homologous bacterin against bovine leptospirosis].
48 adult bovine females dividided into 6 groups were used aimed at characterizing the immune response induced in breastfeeeding cows by an homologous bacterin formulated with different adjuvants. They were intramuscularly administered 2 milliliters of a bacterin formulated with Leptospira interrogans serovars uam, wolffi, hardjo, bratislava, grippotyphosa and panama added with different adjuvants, such as aluminum hydroxide, Freud's complete adjuvant, Freud's incomplete adjuvant, liposoluble vitamins, bacterin plus disparasitization with levamisol. The control group was administred only with bacterin. Immunization took place in 2 occasions at a time interval of 28 days. Blood samples were taken every 7 days during the first month after vaccination, and every 28 days for the next 8 months. All the sera were analyzed by the microscopic agglutination test. The results were transformed into Log10 and they were analyzed by NLIN and GLM of SAS. The period of greater response was estimated by the prediction model (Wood). The bacterin did not produce alteration either in the physiological constants, or in milk production. The serovars of Leptospira interrogans that induced higher titers were uam, hardjo and wolffi. The statistical difference between treatments and between serovars was determined.
Vega
Laura Elena Orozco
LE
Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (INIFAP), México. leorozco@yahoo.com.mx
Flores
Rafael López
RL
Moles y Cervantes
Luis Pedro
LP
Valiente
Jorge Quiroz
JQ
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000917:Antibody Formation; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control*; D003627:Data Interpretation, Statistical; D005260:Female; D005500:Follow-Up Studies; D005620:Freund's Adjuvant / Q000008:administration & dosage; D007273:Injections, Intramuscular; D007921:Leptospira interrogans / Q000145:classification / Q000276:immunology*; D007922:Leptospirosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D013997:Time Factors
D000276:Adjuvants, Immunologic; D001428:Bacterial Vaccines; D005620:Freund's Adjuvant