[Fluorescence characteristics and photophysical parameters of aggregates of light-harvesting chlorophyll a/b complexes].
Changes in the fluorescence characteristics and molecular photophysical parameters induced by disaggregation of light-harvesting chlorophyll a/b complexes isolated from pea were studied. Disaggregation was achieved by increasing the concentration of the detergent triton X-100 (concentration range 0-230 microM, chlorophyll concentration 2.45 microg/ml). Laser fluorimetry methods were used to measure the molecular photophysical parameters. It was shown that the decrease in aggregate size is accompanied by a decrease in fluorescence at 700 nm at 77 K, a fall of the singlet-singlet annihilation rate (by more than two orders), and the growth of fluorescence life time (from 160 ps to 3.2 ns).
Ostroumov
E E
EE
Fadeev
V V
VV
Khristin
M S
MS
Pashchenko
V Z
VZ
Tusov
V B
VB
D002734:Chlorophyll / Q000737:chemistry*; D000077194:Chlorophyll A; D003080:Cold Temperature; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry*; D017830:Octoxynol / Q000737:chemistry*; D018532:Pisum sativum / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D045342:Light-Harvesting Protein Complexes; D010940:Plant Proteins; D002734:Chlorophyll; C037184:chlorophyll b; D017830:Octoxynol; D000077194:Chlorophyll A