title: Identification of differentially expressed genes in microsatellite stable HNPCC and sporadic colon cancer.
abstract: BACKGROUND
  Some sporadic colon cancers and hereditary nonpolyposis colorectal cancer (HNPCC) have been known by a constitutional defect in mismatch gene repair (MMR) and dysfunction in of this MMR system, which lead to an aberrant phenotype that can cause microsatellite instability. However, the clinicopathologic features of still existing microsatellite stable (MSS) colon cancer remain to be investigated.
  
  
  METHODS
  We compared the gene expression patterns of nine tumor tissues of HNPCC and nine tumor tissues of sporadic colon cancer with their adjacent pathologically normal, MSS tissues by modified differential display-polymerase chain reaction, selected four potential marker genes, and confirmed their reproducibility by reverse transcriptase-polymerase chain reaction.
  
  
  RESULTS
  Reg I, MLCK, and MYH11 in tumors of MSS HNPCC showed significant differences in gene expression pattern compared with the adjacent pathologically normal tissues (P = 0.028, P = 0.002, and P = 0.001, respectively). Similar differences in expression patterns for the same set of genes were seen between the sporadic colon cancer group and their adjacent pathologically normal tissues (P = 0.012, P = 0.003, and P = 0.002, respectively).
  
  
  CONCLUSIONS
  We suggest that the three cancer-associated differentially expressed genes in MSS sporadic colon cancer or MSS HNPCC might be potential tumor markers.
authors.affiliation: Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Won-Suk
authors.initials: WS
authors.lastname: Lee
authors.forename: Gilju
authors.initials: G
authors.lastname: Seo
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Shin
authors.forename: Seong Hyeon
authors.initials: SH
authors.lastname: Yun
authors.forename: Haeran
authors.initials: H
authors.lastname: Yun
authors.forename: Naeyun
authors.initials: N
authors.lastname: Choi
authors.forename: Jinseon
authors.initials: J
authors.lastname: Lee
authors.forename: Daesoon
authors.initials: D
authors.lastname: Son
authors.forename: Jisook
authors.initials: J
authors.lastname: Cho
authors.forename: Jhingook
authors.initials: J
authors.lastname: Kim
authors.forename: Yong Beom
authors.initials: YB
authors.lastname: Cho
authors.forename: Ho-Kyung
authors.initials: HK
authors.lastname: Chun
authors.forename: Woo Yong
authors.initials: WY
authors.lastname: Lee
mesh_terms: D003110:Colonic Neoplasms / Q000235:genetics*; D003123:Colorectal Neoplasms, Hereditary Nonpolyposis / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D051847:Lithostathine / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D018995:Myosin Heavy Chains / Q000235:genetics; D009219:Myosin-Light-Chain Kinase / Q000235:genetics; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D051847:Lithostathine; C509903:MYH11 protein, human; C497617:REG1A protein, human; D009219:Myosin-Light-Chain Kinase; D018995:Myosin Heavy Chains