Effects of oxidative stress on the Dolichol content of isolated rat liver cells.
Dolichol, a long-chain polyisoprenoid broadly distributed in all tissues and cellular membranes with unknown function(s), might have a role in free radical metabolism [it accumulates in older tissues and decreases after CCl4 (in liver) or phenylhydrazine (in spleen and liver) administration]. The effects of the NADPH-ADP-Fe system on Dolichol levels in isolated hepatocytes were explored and the time-course of changes was compared with the release of MDA in the incubation medium and the decrease in CoQ 9 and 10 and Vitamin E levels. Results showed that the system increased lipid peroxidation and decreased Dolichol and CoQ levels in-parallel fashions and lowered Vitamin E levels with shorter latency. Meanwhile, no increase in dead cells and no Dolichol release in the medium were detected. In conclusion, an increase in oxidative stress possibly caused a rapid degradation of dolichol by the same (unknown) mechanism responsible for the breakdown of Ubiquinone isoprenoid chains.
Guarini
Monica
M
Centro di Ricerca Interdipartimentale di Biologia e Patologia dell'Invecchiamento dell'Università di Pisa, Italy.
Stabile
Angela
A
Cavallini
Gabriella
G
Donati
Alessio
A
Bergamini
Ettore
E
D000818:Animals; D002478:Cells, Cultured; D004286:Dolichols / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008954:Models, Biological; D018384:Oxidative Stress / Q000502:physiology*; D010545:Peroxides / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014451:Ubiquinone / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism
D004286:Dolichols; D010545:Peroxides; D014451:Ubiquinone; D014810:Vitamin E; D008315:Malondialdehyde