T-tubule formation in cardiacmyocytes: two possible mechanisms?
We have followed the differentiation of transverse (T) tubules and of the associations between sarcoplasmic reticulum (SR) and either the plasmalemma (peripheral couplings) or the T tubules (dyads) in postnatal rat ventricular myocytes using electron microscopy. Dyads and peripheral couplings are collectively called Ca(2+) Release Units (CRUs) because they are the sites at which Ca(2+) is released from the SR. Profiles of T tubules, caveolae and dyads are mostly at the cell edge in early postnatal days and are found with increased frequency in the cell interior during the first two postnatal weeks. Using ferritin to trace continuity of T tubules lumen with the extracellular space, we find that some of T tubules (between approximately 6 and 25%), either singly or within dyads, lack ferritin in their lumen. The percentage of tubules that do not contain ferritin decreases slightly during postnatal differentiation and is not very different at the cells' edges and interior. We propose that T tubules form as invaginations of the plasmalemma that penetrate inward driven by accrual of membrane lipids and specific proteins. This occurs by a dual mechanism: either by the independent flow of SR and T tubule proteins into the two separate membranes or by the fusion of preformed vesicle tandems into the dyads. Most of the CRUs (approximately 86%) are constituted by peripheral couplings and ferritin containing dyads, thus constituting CRUs in which Ca(2+ )release from the SR is initiated by a membrane depolarization. In the remaining CRUs, activation of Ca(2+) release must be dependent on some other mechanisms.
Di Maio
Alessandro
A
Department of Cell and Developmental Biology, School of Medicine, University of Pennsylvania, Anat/Chem Building, Room B42, Philadelphia, PA 19104-6058, USA. dimaio@mail.med.upenn.edu
Karko
Kimberly
K
Snopko
Rose M
RM
Mejía-Alvarez
Rafael
R
Franzini-Armstrong
Clara
C
D000818:Animals; D000831:Animals, Newborn; D020013:Calcium Signaling / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D005293:Ferritins / Q000378:metabolism; D006321:Heart / Q000033:anatomy & histology / Q000254:growth & development*; D007425:Intracellular Membranes / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008870:Microtubules / Q000648:ultrastructure*; D009206:Myocardium / Q000648:ultrastructure*; D032383:Myocytes, Cardiac / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012519:Sarcoplasmic Reticulum / Q000648:ultrastructure*
D005293:Ferritins