Development of MAb-based immunochromatographic assay for cadmium from biological samples.
Cadmium (Cd) is a general environmental pollutant of increasing global concern. In 2005 the joint FAO/WHO Codex Alimentarius Commission proposed new international food legislation for low-level Cd contaminants. In this study we demonstrate the use of novel monoclonal antibody (MAb) to Cd-EDTA in an immunochromatography (IC) format for the quick testing for trace Cd. This IC device could detect 0.3 microg kg(-1) (0-3 ppb) Cd. Contaminated Zn, Mn, Mg, and Cu, which would interfere the measurement of Cd by cross reaction to the MAb, could be removed by using a column that could separate trace Cd from other heavy metals in the extract of brown rice.
Sasaki
Kazuhiro
K
Central Res. Inst. of Electr. Power Ind, 270-1194 Japan. k-sasaki@criepi.denken.or.jp
Tawarada
Kei
K
Okuhata
Hirosi
H
Miyasaka
Hitoshi
H
Okuyama
Akira
A
Maruyama
Yukinao
Y
Ohmura
Naoya
N
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002104:Cadmium / Q000032:analysis* / Q000276:immunology*; D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D012275:Oryza / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D002104:Cadmium