title: [Identification and molecular study on vibrio cholerae in sea products].
abstract: OBJECTIVE
  To investigate the serologic type, phage-biotype and toxic factor of Vibrio cholerae isolated from different sea products, analyze the relation between the Vibrio cholerae in sea products and cholera epidemiology, and provide references for forecasting cholera epidemic situation and drawing out a preventing plan.
  
  
  METHODS
  The biotype of strains isolated was analyzed by using type and phage-biotype serological methods. The toxic gene was detected by PCR.
  
  
  RESULTS
  The constituent ratio of V. cholerae O139, Ogawa and Inaba were, respectively, 48.44%, 20.31% and 31.25% in 64 strains of V. cholerae. The result of phage-biotype showed that the 26 strains of V. cholerae O1 were all non-epidemic strains. The result of toxic gene detecting showed that positive rate of V. cholerae O139 was higher than those of Ogawa and Inaba.
  
  
  CONCLUSIONS
  The positive rate of toxic gene in V. cholerae O139 was high and the V. cholerae O139 was mainly in turtle, breed aquatics water and crustacean, so these sea products were the important sectors in cholera prevention and control.
authors.affiliation: Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Zhao-rui
authors.initials: ZR
authors.lastname: Chang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Duo-chun
authors.initials: DC
authors.lastname: Wang
authors.forename: Hao-jie
authors.initials: HJ
authors.lastname: Zhong
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.forename: Lu
authors.initials: L
authors.lastname: Ran
authors.forename: Mao-wu
authors.initials: MW
authors.lastname: Wang
authors.forename: Zi-jun
authors.initials: ZJ
authors.lastname: Wang
authors.forename: Biao
authors.initials: B
authors.lastname: Kan
mesh_terms: D000818:Animals; D001434:Bacteriophage Typing; D004269:DNA, Bacterial / Q000235:genetics; D017747:Seafood / Q000382:microbiology*; D012703:Serotyping; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D041241:Vibrio cholerae O1 / Q000302:isolation & purification; D041242:Vibrio cholerae O139 / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial