title: Modulation of endothelial responses by the stromal microenvironment: effects on leucocyte recruitment.
abstract: Vascular endothelial cells play a pivotal role in regulating leucocyte recruitment during inflammation, and their responses can be modulated by their local environment, including cells of the tissue stroma. We have developed a model system to examine how the communication between endothelial cells and fibroblasts regulates the recruitment of leucocytes and their subsequent subendothelial fate. Here, we describe a novel co-culture filter-based flow assay and highlight the ability of synovial fibroblasts obtained from chronically inflamed tissue to promote leucocyte recruitment to otherwise 'resting' endothelial cells.
authors.affiliation: Department of Physiology, The Medical School, The University of Birmingham, Birmingham B15 2TT, U.K. helenmcgettrick@yahoo.com
authors.forename: H M
authors.initials: HM
authors.lastname: McGettrick
authors.forename: A
authors.initials: A
authors.lastname: Filer
authors.forename: G E
authors.initials: GE
authors.lastname: Rainger
authors.forename: C D
authors.initials: CD
authors.lastname: Buckley
authors.forename: G B
authors.initials: GB
authors.lastname: Nash
mesh_terms: D018920:Coculture Techniques; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D007962:Leukocytes / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology*