Probing paeonol-pluronic polymer interactions by 1H NMR spectroscopy.
By using a combination of 1H NMR spectroscopy, two-dimensional heteronuclear single-quantum coherence-resolved (1)H{(13)C} and homonuclear rotating-frame Overhauser enhancement NMR correlation experiments with diffusion ordered spectroscopy (DOSY), the location and distribution of a hydrophobic drug, paeonol, have been established with respect to the methyl groups of the poly(ethylene oxide)-poly(propylene oxide) -poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymer. The interaction between them is adjustable according to the different temperature-dependent hydrophilicities or hydrophobicities of the triblock copolymer components. On the other hand, such interactions influence the self-assembly properties of the block copolymer amphiphiles in solution. The amount of anhydrous methyl groups of PPO segments shows an increase with increasing paeonol concentration. It was also demonstrated that the shell-crosslinking of the Pluronic polymer has an effect in increasing the amount of anhydrous methyl groups and thus increasing the hydrophobicity of Pluronic micelles. This might be the deeper reason underlying the increase in drug-loading capacity and prolongation in release time of Pluronic micelles for drug delivery after the shell-crosslinking. Changes in self-diffusion coefficients of paeonol with varying copolymer concentrations and types were also determined by the diffusion-based NMR DOSY technique, and values of K(a), DeltaG, and n were calculated.
Ma
Jun-He
JH
Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Graduate School of Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Guo
Chen
C
Tang
Ya-Lin
YL
Zhang
Hong
H
Liu
Hui-Zhou
HZ
D000098:Acetophenones / Q000737:chemistry*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011409:Propylene Glycols / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics
D000098:Acetophenones; D008823:Micelles; C116176:PEO-PPO-PEO; D011108:Polymers; D011409:Propylene Glycols; C012504:polypropylene glycol; C013638:paeonol; D011092:Polyethylene Glycols