Molecular detection of Xenostrobus securis and Mytillus galloprovincialis larvae in Galician Coast (Spain).
The mussel species Xenostrobus securis from New Zealand was detected in the Spanish coast recently, in the mouth of the Verdugo River into the Vigo Ria. In view of the great importance of the farm mussel sector in this region, the presence of this alien species greatly concerned producers and administration authorities, because of its potential medium- or long-term effects on the autochthonous species, Mytilus galloprovincialis, an important marine resource widely exploited in this location. The goal of this study was to develop a DNA-based technique to identify X. securis and M. galloprovincialis larvae in plankton samples, which would allow monitoring for the presence of X. securis in different points of the Vigo Ria. The techniques used were simplex and multiplex polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and fragment analysis. The application of this system to planktonic samples could be an effective means to assess the presence of the alien species, allowing monitoring if its dispersion is increasing, or on the contrary, if its distribution is restricted to the mouth of the Verdugo River, where X. securis was first detected. In addition, the application of this system at different times could be useful to assess the presence of larvae of these two species in the plankton.
Santaclara
Francisco J
FJ
Area of Molecular Biology and Biotechnology, ANFACO-CECOPESCA, Vigo 36310, Pontevedra, Spain. fransanta@anfaco.es
Espiñeira
Montserrat
M
Vieites
Juan M
JM
D000818:Animals; D049872:Bivalvia / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D007814:Larva / Q000145:classification / Q000235:genetics / Q000502:physiology; D049879:Mytilidae / Q000145:classification / Q000235:genetics* / Q000502:physiology; D049878:Mytilus / Q000145:classification / Q000235:genetics / Q000502:physiology; D009792:Oceans and Seas; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012623:Seawater / Q000032:analysis*; D012680:Sensitivity and Specificity; D013030:Spain; D013045:Species Specificity
D017931:DNA Primers; D012337:RNA, Ribosomal, 18S