title: [Isolation and structure identification of chemical constituents from the skin of Bufo bufo gargarizans].
abstract: The skin of Bufo bufo gargarizans, originated from Bufo bufo gargarizans Cantor (Bufonidae), is widely used in traditional Chinese medicine for the treatment of hepatoma, lung cancer and etc. The preparation of the aqueous components has significant therapeutic effect against the digestive tract cancer. The water-soluble chemical constituents in the skin of Bufo bufo gargarizans were then investigated to make clear the active compounds. Six compounds were isolated and purified by recrystallization and column chromatography on silica gel and ODS, their structures were elucidated as 4-amido-3-hydroxymethyl-cyclooctylamidezotetra-alpha-furanone (I), bufogargarizanine C (II), bufothionine (III), dehydrobufotenine hydrobromide (IV), suberic acid (V) and succinic acid (VI) on the basis of physicochemical properties and spectral data (UV, IR, 1H NMR, 13C NMR and MS). Of the above compounds, compounds I and II are new compounds and named bufogargarizanine B and C, respectively.
authors.affiliation: Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Dai
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Gao
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Wang
authors.forename: Wei-Hao
authors.initials: WH
authors.lastname: Wang
mesh_terms: D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry / Q000302:isolation & purification*; D002023:Bufo bufo; D002210:Caprylates / Q000737:chemistry / Q000302:isolation & purification; D003998:Dicarboxylic Acids / Q000737:chemistry / Q000302:isolation & purification; D008516:Medicine, Chinese Traditional; D008968:Molecular Conformation; D015394:Molecular Structure; D012867:Skin / Q000737:chemistry*
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D002210:Caprylates; D003998:Dicarboxylic Acids; C541960:bufogargarizanine B; C541961:bufogargarizanine C; C005738:suberic acid