title: Preconditioning with oxygen attenuates rat renal ischemia-reperfusion injury.
abstract: BACKGROUND
  Short time pretreatment with oxygen is reported to be protective against subsequent ischemia-reperfusion (IR) injury of heart and spinal cord in some animal models. The purpose of this study was to investigate the effects of pre-exposure to hyperoxic environment on rat renal IR injury for the first time.
  
  
  METHODS
  The effects of 1 h/d pretreatment with oxygen (>or=95%) for 5 days on a right nephrectomized rat model of renal IR injury was investigated by comparing creatinine clearance, fractional excretion of sodium, plasma creatinine, blood urea nitrogen, and histological injury scores among three groups: IR (40 min ischemia-24 h reperfusion), sham (no IR), and hyperoxia (5 days intermittent pretreatment with oxygen + IR).
  
  
  RESULTS
  Intermittent pretreatment with oxygen resulted in significant improvement of creatine clearance and fractional excretion of sodium (P <or= 0.05). Plasma creatinine above 175 micromol/L or blood urea nitrogen beyond 26 mmol/L was significantly less frequent in hyperoxia than IR group (P < 0.05). Jablonski histological injury score was also significantly lower in hyperoxia compared to IR group (P < 0.05) and hyperoxic preconditioning significantly reduced the frequency of massive proximal tubular necrosis (12.5% versus 75%, P < 0.05).
  
  
  CONCLUSIONS
  The present findings demonstrate that intermittent pre-exposure to hyperoxic environment can reduce subsequent renal IR injury.
authors.affiliation: Research Center for Trauma, Baqiyatallah University of Medical Sciences, Tehran, Iran. rasoulian@irimc.org
authors.forename: Bahram
authors.initials: B
authors.lastname: Rasoulian
authors.forename: Hassan
authors.initials: H
authors.lastname: Mohammadhosseniakbari
authors.forename: Mehri
authors.initials: M
authors.lastname: Kadkhodaee
authors.forename: Mahmood
authors.initials: M
authors.lastname: Mofid
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Baqeri
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Bigdeli
authors.forename: Asghar
authors.initials: A
authors.lastname: Ghasemi
authors.forename: Hassan Ali
authors.initials: HA
authors.lastname: Mohebbi
authors.forename: Alireza
authors.initials: A
authors.lastname: Asgari
authors.forename: Ali
authors.initials: A
authors.lastname: Khoshbaten
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D005740:Gases / Q000627:therapeutic use*; D019194:Ischemic Preconditioning / Q000379:methods; D007668:Kidney / Q000098:blood supply*; D007674:Kidney Diseases / Q000517:prevention & control*; D008297:Male; D010100:Oxygen / Q000627:therapeutic use*; D010102:Oxygen Inhalation Therapy; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D012964:Sodium / Q000652:urine
chemical_list: D005740:Gases; D012964:Sodium; D003404:Creatinine; D010100:Oxygen