Characterization of cecal microbiota and response to an orally administered lactobacillus probiotic strain in the broiler chicken.
A probiotic Lactobacillus strain was given in drinking water to young broiler chickens from 1 to 19 days of age. Cecal contents were collected from 4- and 19-day-old chickens in treated and control groups. Enumeration of bacteria by culture on selective media showed a decrease in Clostridium perfringens carriage in the 4-day-old treated chickens, whereas coliforms and Lactobacillus populations were not significantly affected by the treatment. Fluorescent in situ hybridization analysis with 7 phylogenetic probes targeting the major groups of intestinal bacteria revealed that the Clostridium coccoides group accounted for more than 50% of the total bacteria in the cecum of 4-day-old chickens, whereas the bacterial community of 19-day-old chickens evolved towards a more diverse microbiota with Faecalibacterium prausnitzii (36%) and C. coccoides (22%) groups representing the predominant bacteria. No effect of the Lactobacillus strain supplementation was observed in the composition of the cecal microbiota assessed by fluorescent in situ hybridization with the 7 probes. Nevertheless, profiling of the cecal microbiota using temporal temperature gradient gel electrophoresis in combination with principal component analysis demonstrated an impact of the probiotic treatment on the overall bacterial community as well as on the Lactobacillus population.
Gérard
Philippe
P
Unité d'Ecologie et Physiologie du Système Digestif, Institut National de la Recherche Agronomique, Jouy-en-Josas, France. philippe.gerard@jouy.inra.fr
Brézillon
Christophe
C
Quéré
Florence
F
Salmon
Annick
A
Rabot
Sylvie
S
D000284:Administration, Oral; D000818:Animals; D002432:Cecum / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology*; D003016:Clostridium perfringens / Q000254:growth & development; D015169:Colony Count, Microbial; D004587:Electrophoresis, Agar Gel; D004755:Enterobacteriaceae / Q000254:growth & development; D005434:Flow Cytometry; D017404:In Situ Hybridization, Fluorescence; D007778:Lactobacillus / Q000254:growth & development*; D025341:Principal Component Analysis; D019936:Probiotics / Q000008:administration & dosage*; D013696:Temperature