title: A crucial role for dendritic cell (DC) IL-10 in inhibiting successful DC-based immunotherapy: superior antitumor immunity against hepatocellular carcinoma evoked by DC devoid of IL-10.
abstract: The dendritic cell (DC)-based tumor immunotherapy has been a new promise of cure for cancer patients, but animal studies and clinical trials have thus far only shown limited success, especially in treating established tumors. Certain immunosuppressive mechanisms triggered by tumor cells or the derivatives are believed to be a major obstacle. We studied the role of DC-derived IL-10 and its negative impact on vaccine efficacy in mouse models. Liver tumor cells were injected via the portal vein, giving rise to disseminated intrahepatic tumors, or s.c. to form solid but extrahepatic tumors. Bone marrow-derived DCs were generated from normal or IL-10-deficient mice and used as the vector to deliver tumor Ags. We demonstrate here that DCs devoid of IL-10, a potent immunosuppressive cytokine, are superior over conventional DCs in triggering antitumor immunity. The IL-10(-/-)DCs were highly immunogenic, expressed enhanced levels of surface MHC class II molecules, and secreted increased amounts of Th1-related cytokines. By inducing tumor-specific killing and through the establishment of immunological memory, the vaccines delivered by IL-10(-/-)DCs could evoke strong therapeutic and protective immunity against hepatocellular carcinoma in the mouse models. These findings will have great clinical impact once being translated into the treatment of malignant, and potentially infectious, diseases in humans.
authors.affiliation: Department of Pathology, University of Honk Kong, China.
authors.forename: Yu-Xiao
authors.initials: YX
authors.lastname: Chen
authors.forename: Kwan
authors.initials: K
authors.lastname: Man
authors.forename: Guang Sheng
authors.initials: GS
authors.lastname: Ling
authors.forename: Yichen
authors.initials: Y
authors.lastname: Chen
authors.forename: Bai-Shun
authors.initials: BS
authors.lastname: Sun
authors.forename: Qiao
authors.initials: Q
authors.lastname: Cheng
authors.forename: On Hong
authors.initials: OH
authors.lastname: Wong
authors.forename: Chi-Kin
authors.initials: CK
authors.lastname: Lo
authors.forename: Irene Oi-Lin
authors.initials: IO
authors.lastname: Ng
authors.forename: Li Chong
authors.initials: LC
authors.lastname: Chan
authors.forename: George K
authors.initials: GK
authors.lastname: Lau
authors.forename: Chen-Lung Steve
authors.initials: CL
authors.lastname: Lin
authors.forename: Fanglu
authors.initials: F
authors.lastname: Huang
authors.forename: Fang-Ping
authors.initials: FP
authors.lastname: Huang
mesh_terms: D000818:Animals; D019496:Cancer Vaccines; D006528:Carcinoma, Hepatocellular / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D007156:Immunologic Memory / Q000276:immunology; D007167:Immunotherapy; D016753:Interleukin-10 / Q000172:deficiency / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009368:Neoplasm Transplantation; D010641:Phenotype
chemical_list: D019496:Cancer Vaccines; D016753:Interleukin-10