Detection, isolation, and identification of cadmium-resistant bacteria based on PCR-DGGE.
This study focused on the screening of cadmium-resistant bacterial strains from Pb-Zn tailing. We investigated the diversity of microbial community inhabiting Dong-san-cha Pb-Zn tailing in Beijing, China, by polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene of bacterial strain, and found two dominant strains in the DGGE profile. Using special culture media, we isolated two strong cadmium-resistant bacterial strains. On the basis of morphological, physiological, and biochemical characteristics, BIOLOG, and 16S rDNA sequencing, the two strains were identified as Bacillus cereus and Enterobacter cloacae. Minimal inhibitory concentrations (MICs) of heavy metals for the bacteria were determined. E. cloacae showed higher MIC values for heavy metals and a larger range of antibiotic resistance than B. cereus.
Hu
Qing
Q
Department of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. huqing@rcees.ac.cn
Dou
Min-Na
MN
Qi
Hong-Yan
HY
Xie
Xiang-Ming
XM
Zhuang
Guo-Qiang
GQ
Yang
Min
M
D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification* / Q000648:ultrastructure; D001483:Base Sequence; D002104:Cadmium / Q000494:pharmacology*; D017931:DNA Primers; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D002104:Cadmium