title: Sonoran propolis: chemical composition and antiproliferative activity on cancer cell lines.
abstract: In this study, we have analyzed the chemical composition and antiproliferative activity of propolis from three different arid and semiarid regions of Sonora, Mexico. We identified and quantitated the main chemical constituents of propolis by HPLC-MS. The most abundant constituents of propolis were pinocembrin, pinobanksin 3-acetate, and chrysin. Sonoran propolis had a strong antiproliferative activity on both murine and human cancer cell lines in a concentration-dependent manner. The propolis constituents CAPE, galangin, xanthomicrol and chrysin showed significant antiproliferative activity on most of the cancer cells tested. DNA harvested from cancer cell cultures treated with Sonoran propolis exhibited a ladder of internucleosomal DNA cleavage characteristic of apoptosis. In summary, we have identified and quantitated the main constituents of Sonoran propolis. These propolis samples possess a strong antiproliferative activity on cancer cell lines.
authors.affiliation: Laboratory of Biopolymers, Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo, Sonora, México. Jahernan@cascabel.ciad.mx
authors.forename: Javier
authors.initials: J
authors.lastname: Hernandez
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Goycoolea
authors.forename: Jael
authors.initials: J
authors.lastname: Quintero
authors.forename: Ana
authors.initials: A
authors.lastname: Acosta
authors.forename: Marco
authors.initials: M
authors.lastname: Castañeda
authors.forename: Zaira
authors.initials: Z
authors.lastname: Dominguez
authors.forename: Refugio
authors.initials: R
authors.lastname: Robles
authors.forename: Luz
authors.initials: L
authors.lastname: Vazquez-Moreno
authors.forename: Enrique F
authors.initials: EF
authors.lastname: Velazquez
authors.forename: Humberto
authors.initials: H
authors.lastname: Astiazaran
authors.forename: Efraín
authors.initials: E
authors.lastname: Lugo
authors.forename: Carlos
authors.initials: C
authors.lastname: Velazquez
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D008800:Mexico; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology*; D011429:Propolis / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D011429:Propolis