Consumption of Camembert cheese stimulates commensal enterococci in healthy human intestinal microbiota.
Enterococci are natural inhabitants of the human gastrointestinal tract and the main Gram-positive and facultative anaerobic cocci recovered in human faeces. They are also present in a variety of fermented dairy and meat products, and some rare isolates are responsible for severe infections such as endocarditis and meningitis. The aim of the present study was to evaluate the effect of Camembert cheese consumption by healthy human volunteers on the faecal enterococcal population. A highly specific real-time quantitative PCR approach was designed and used to type enterococcal species in human faeces. Two species were found, Enterococcus faecalis and Enterococcus faecium, and only the Enterococcus faecalis population was significantly enhanced after Camembert cheese consumption, whereas Escherichia coli population and the dominant microbiota remained unaffected throughout the trial.
Firmesse
Olivier
O
Unité d'Ecologie et Physiologie du Système Digestif, INRA, Jouy-en-Josas, France.
Rabot
Sylvie
S
Bermúdez-Humarán
Luis G
LG
Corthier
Gérard
G
Furet
Jean-Pierre
JP
D002611:Cheese / Q000382:microbiology*; D015169:Colony Count, Microbial / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D013293:Enterococcus faecalis / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D016984:Enterococcus faecium / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D004926:Escherichia coli / Q000254:growth & development; D005243:Feces / Q000382:microbiology; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods
D004269:DNA, Bacterial