title: Oocyte-specific G-protein-coupled receptor 3 (GPR3): no perturbations found in 82 women with premature ovarian failure (first report).
abstract: The oocyte-specific G-protein-coupled receptor 3 (GPR3) gene is essential in maintaining meiotic arrest in mouse oocytes. Disruption of GPR3 results in early depletion of oocytes and thus premature ovarian aging. To determine if mutations of the GPR3 gene were present in 82 predominantly North American caucasian women with premature ovarian failure (POF), we used denaturing high-performance liquid chromatography and DNA sequencing to detect sequence variants. None of the 82 POF samples showed perturbations of significance. We conclude that perturbations in GPR3 are not a common explanation for POF in this population.
authors.affiliation: Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, Texas, USA. ekovanci@bcm.edu
authors.forename: Ertug
authors.initials: E
authors.lastname: Kovanci
authors.forename: Joe Leigh
authors.initials: JL
authors.lastname: Simpson
authors.forename: Paula
authors.initials: P
authors.lastname: Amato
authors.forename: Jan
authors.initials: J
authors.lastname: Rohozinski
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Heard
authors.forename: Colin E
authors.initials: CE
authors.lastname: Bishop
authors.forename: Sandra A
authors.initials: SA
authors.lastname: Carson
mesh_terms: D000328:Adult; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D009865:Oocytes / Q000378:metabolism*; D010865:Pilot Projects; D016649:Primary Ovarian Insufficiency / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism
chemical_list: C092794:GPR3 protein, human; D043562:Receptors, G-Protein-Coupled