Development of a novel ELISA for serodiagnosis of Leptospirosis and additional detection of pathogenic Leptospira by polymerase chain reaction for veterinary routine diagnostics.
A multi-serovar ELISA based on the outer membrane Lipoprotein L41 (LipL41) of pathogenic Leptospira was developed to increase sensitivity by using a single test antigen. A sensitivity of 99 % and a specifity of 92 % could be achieved. The established diagnostic polymerase chain reaction is also able to detect fast and reliably pathogenic Leptospira in different clinical samples.
Theodoridis
Dimitrios
D
IVD GmbH, School of Veterinary Medicine Hannover, Germany. theodoridis@ivd-gmbh.de
Böhmer
Jan
J
Homuth
Matthias
M
Strutzberg-Minder
Katrin
K
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005849:Gerbillinae; D007919:Leptospira / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D007922:Leptospirosis / Q000175:diagnosis / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*