[Study of the possible mechanism of effects of carbon disulfide on c-myc expression in rat testicles].
OBJECTIVE
To survey the expression of c-myc of teticles in rats exposed to carbon disulfide (CS2) and the possible mechanism of effects on it.


METHODS
36 wistar male rats were randomly divided into six groups. Four groups were exposed to CS2 at the dose of 0, 50, 250 and 1250mg/m3. The other two groups were treated at the dose of 1250 mg/ m3 CS2 plus VitE (250mg/kg diet) and VitE (250mg/kg diet) respectively. After 10-week esposures, rats were sacrificed and the SOD activity and MDA content and the c-myc expressions in testicles were determined.


RESULTS
When compared with the control group, SOD activities of testicles in the exposure group were decreased significantly (P < 0.01), MDA contents of testicles were increased significantly at the concentration of 1250mg/m3 CS2 (P < 0.01). The expression of c-myc of testicles present biphasic, and decreased at the concentraton of 50mg/m3 CS2 and increased at the other concentrations and higher than these of controls at the concentration of 1250mg/m3 CS2 significantly (P < 0.05). After treatment of CS2 combined with antioxidant VitE, SOD activity of testicles increased (P < 0.05), MDA content of testicles decreased (P < 0.01) and c-myc expression of testicles decreased consequently (P < 0.05).


CONCLUSIONS
the modulation of c-myc expression in testicles of rats treated with CS2 could be associated with lipid peroxidation induced by CS2.
Duan
Yanying
Y
Department of Environmental and Occupational Health, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China.
Chen
Guoyuan
G
Ji
Jiajia
J
Lu
Yan
Y
D000818:Animals; D002246:Carbon Disulfide / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000378:metabolism*
D016271:Proto-Oncogene Proteins c-myc; D013482:Superoxide Dismutase; D002246:Carbon Disulfide