Proline homozygosity in codon 72 of TP53 is a factor of susceptibility to nasopharyngeal carcinoma in Tunisia.
A common polymorphism at codon 72 of TP53, the gene encoding the tumor suppressor protein p53, encodes either arginine or proline. These variants may be associated with tumor susceptibility since they interfere with the ability of TP53 to activate apoptosis, and might account for ethnic variation in cancer frequency. Using a polymerase chain reaction-restriction fragment length polymorphism assay, we tested peripheral blood samples from 115 patients with nasopharyngeal carcinoma (NPC) and from 83 healthy individuals. Patients with NPC (Arg/Arg = 38.26%, Arg/Pro = 41.73%, and Pro/Pro = 20%) showed a significantly different percentage of the Pro/Pro genotype, compared with the control population (Arg/Arg = 39%, Arg/Pro = 54%, and Pro/Pro = 7%) (P = 0.0307). No significant difference was observed between TP53 codon 72 polymorphism and age, sex, histological grade, and metastasis. These results provide evidence that individuals with the Pro/Pro genotype have an increased risk of developing NPC in Tunisia.
Hadhri-Guiga
Boutheina
B
Laboratory for Molecular Genetics of Eukaryotes, Biotechnology Center of Sfax, Ministry for Research in Science, Technology, and Development, BP W 3018, Sfax, Tunisia.
Toumi
Nabil
N
Khabir
Abdelmajid
A
Sellami-Boudawara
Tahia
T
Ghorbel
Abdelmoonem
A
Daoud
Jamel
J
Frikha
Mounir
M
Gargouri
Ali
A
Mokdad-Gargouri
Raja
R
D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D002277:Carcinoma / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D002648:Child; D003062:Codon / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011392:Proline / Q000235:genetics*; D012016:Reference Values; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D014416:Tunisia
D003062:Codon; D016159:Tumor Suppressor Protein p53; D011392:Proline