Dietary sesamin suppresses aortic NADPH oxidase in DOCA salt hypertensive rats.
1. Dietary sesamin, a sesame lignan, is known to suppress the development of experimental hypertension in rats partly through its inhibitory effect on vascular O(2)(-) production. Therefore, in the present study, we examined whether sesamin feeding had any effect on vascular NADPH oxidase using aortas from deoxycorticosterone acetate (DOCA) salt hypertensive rats. 2. After a 5 week feeding and treatment period, aortic O(2)(-) production and NADPH oxidase activity were measured using the lucigenin assay. Reverse transcription-polymerase chain reaction was performed to analyse aortic expression of NADPH oxidase subunit (p22phox, gp91phox, Nox1 and Nox4) mRNA. 3. Sesamin feeding markedly suppressed DOCA salt-induced hypertension and significantly decreased aortic O(2)(-) production. DOCA salt treatment increased NADPH oxidase activity and elevated aortic mRNA expression of p22phox, gp91phox, Nox1 and Nox4. Sesamin feeding abolished the increase in NADPH oxidase activity and, furthermore, significantly suppressed increases in p22phox, gp91phox and Nox1 mRNA expression. 4. In conclusion, dietary sesamin prevented DOCA salt-induced increases in NADPH oxidase activity and subunit mRNA expression. These effects seem to be involved in the anti-oxidant and antihypertensive effects of sesamin.
Nakano
Daisuke
D
Laboratory of Pathological and Molecular Pharmacology, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan.
Kurumazuka
Daisuke
D
Nagai
Yukiko
Y
Nishiyama
Akira
A
Kiso
Yoshinobu
Y
Matsumura
Yasuo
Y
D000818:Animals; D001011:Aorta / Q000201:enzymology*; D003497:Cyclic N-Oxides / Q000627:therapeutic use; D003900:Desoxycorticosterone / Q000009:adverse effects*; D019587:Dietary Supplements; D004149:Dioxoles / Q000494:pharmacology*; D006973:Hypertension / Q000139:chemically induced / Q000188:drug therapy*; D017705:Lignans / Q000494:pharmacology*; D008297:Male; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013113:Spin Labels
D003497:Cyclic N-Oxides; D004149:Dioxoles; D017705:Lignans; D013113:Spin Labels; D003900:Desoxycorticosterone; D019255:NADPH Oxidases; C054125:sesamin; C001803:tempol