Novel sequence-responding fluorescent oligoDNA probe bearing a silylated pyrene molecule.
A novel fluorescent phosphoramidite derivative of dimethylsilylated pyrene was prepared and incorporated into oligoDNA. The fluorescent oligoDNA exhibited marked fluorescent signal upon binding to the fully matched complementary DNA strand, however, the signal was strongly quenched in the single-stranded form as well as in the duplex having mismatched base pair at the terminus of the duplex-forming region.
Sekiguchi
Tohru
T
Graduate School of Chemistry and Chemical Biology, Gunma University, 1-5-1 Tenjin-cho, Kiryu City 376-8515, Japan.
Ebara
Yumiko
Y
Moriguchi
Tomohisa
T
Shinozuka
Kazuo
K
D000577:Amides / Q000737:chemistry; D001483:Base Sequence; D015342:DNA Probes / Q000737:chemistry*; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D010756:Phosphoric Acids / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013696:Temperature
D000577:Amides; D015342:DNA Probes; D010756:Phosphoric Acids; D011721:Pyrenes; C030984:pyrene; C011067:phosphoramidic acid