title: Photoaging-associated changes in epidermal proliferative cell fractions in vivo.
abstract: The epidermis is a dynamic epithelium with constant renewal throughout life. Epidermal homeostasis depends on two types of proliferative cells, keratinocyte stem cells (KSCs), and transit amplifying (TA) cells. In the case of chronologic aging, levels of KSCs tend to decrease and change functionally. However, little is known about the effect of photoaging on epidermal proliferative subtype populations. The aim of this study was to validate involucrin/beta1-integrin ratio as a molecular marker of epidermal photoaging, and to investigate the effects of photoaging caused by chronic UV exposure on the proliferative subtype populations. A total of 15 male volunteers (age range 20-24 and 77-85 years, Fitzpatrick skin phototype III-IV) provided sun-exposed and sun-protected skin samples for real-time RT-PCR, Western blot analysis and immunostaining. Fractional changes in proliferative subtype populations in photoaged and chronologically aged skins were analyzed by flow cytometry. The expression of beta1-integrin was found to be significantly reduced in photoaged skin and ratios of the expressions of involucrin to beta1-integrin were increased 2.6-fold only in elderly subjects. Interestingly, immunostaining of the sun-exposed skins of elderly subjects showed aberrant beta1-integrin expression over the basal layer and greater numbers of Ki-67-positive cells than in sun-protected buttock skin. Flow cytometric analysis revealed that the proportion of KSCs to TA cells was reversed in sun-exposed and sun-protected skins of elderly subjects. Our results suggest that KSC numbers may be lower in photoaged skin than in chronologically aged skin and could be applied to hyperplastic pattern of photoaging. These findings suggest that the epidermis of photoaged skin is impaired in terms of its proliferative potential by attempting to repair chronic UV exposure and that photoaging may be associated with alteration in the two proliferative cell fractions.
authors.affiliation: Department of Dermatology, Seoul National University College of Medicine, Seoul, South Korea.
authors.forename: Oh Sang
authors.initials: OS
authors.lastname: Kwon
authors.forename: Hyeon Gyeong
authors.initials: HG
authors.lastname: Yoo
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Han
authors.forename: Se Rah
authors.initials: SR
authors.lastname: Lee
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Chung
authors.forename: Hee Chul
authors.initials: HC
authors.lastname: Eun
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D002454:Cell Differentiation / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects*; D004817:Epidermis / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism; D015603:Keratinocytes / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects; D008297:Male; D011498:Protein Precursors / Q000378:metabolism; D015595:Skin Aging / Q000473:pathology* / Q000528:radiation effects; D013234:Stem Cells / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D015415:Biomarkers; D019012:Integrin beta1; D011498:Protein Precursors; C032253:involucrin