title: Ceramide kinase expression is altered during macrophage-like cell differentiation of the leukemia cell line HL-60.
abstract: Ceramide kinase (CerK) has important roles in leukocyte functions, including the role in degranulation of mast cells and the phagocytosis of polymorphonuclear leukocytes, so its expression levels should be strictly regulated. Here, we report that the mRNA expression and enzyme activity of CerK were decreased during macrophage-like cell differentiation of the leukemia cell line HL-60, yet neither was altered during granulocytic differentiation of the same cells. Our findings demonstrate that HL-60 cells are useful for studying CerK functions in leukocyte differentiation, and they also suggest that CerK might have an important role in such differentiation.
authors.affiliation: Laboratory of Biomembrane and Biofunctional Chemistry, Faculty of Pharmaceutical Science and Faculty of Advanced Life Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Date
authors.forename: Susumu
authors.initials: S
authors.lastname: Mitsutake
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Igarashi
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000201:enzymology* / Q000473:pathology*; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D014212:Tretinoin; D017853:Phosphotransferases (Alcohol Group Acceptor); C066324:ceramide kinase; D013755:Tetradecanoylphorbol Acetate