title: Evaluation of a protocol for metabolic profiling studies on human blood plasma by combined ultra-performance liquid chromatography/mass spectrometry: From extraction to data analysis.
abstract: The investigation presented here describes a protocol designed to perform high-throughput metabolic profiling analysis on human blood plasma by ultra-performance liquid chromatography/mass spectrometry (UPLC/MS). To address whether a previous extraction protocol for gas chromatography (GC)/MS-based metabolic profiling of plasma could be used for UPLC/MS-based analysis, the original protocol was compared with similar methods for extraction of low-molecular-weight compounds from plasma via protein precipitation. Differences between extraction methods could be observed, but the previously published extraction method was considered the best. UPLC columns with three different stationary phases (C8, C18, and phenyl) were used in identical experimental runs consisting of a total of 60 injections of extracted male and female plasma samples. The C8 column was determined to be the best for metabolic profiling analysis on plasma. The acquired UPLC/MS data of extracted male and female plasma samples was subjected to principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA). Furthermore, a strategy for compound identification was applied here, demonstrating the strength of high-mass-accuracy time-of-flight (TOF)/MS analysis in metabolic profiling.
authors.affiliation: Umeå Plant Science Center, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-901 87 Umeå, Sweden.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Bruce
authors.forename: Pär
authors.initials: P
authors.lastname: Jonsson
authors.forename: Henrik
authors.initials: H
authors.lastname: Antti
authors.forename: Olivier
authors.initials: O
authors.lastname: Cloarec
authors.forename: Johan
authors.initials: J
authors.lastname: Trygg
authors.forename: Stefan L
authors.initials: SL
authors.lastname: Marklund
authors.forename: Thomas
authors.initials: T
authors.lastname: Moritz
mesh_terms: D000596:Amino Acids / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D003596:Cytosine / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000097:blood; D010743:Phospholipids / Q000097:blood; D010949:Plasma / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: D000596:Amino Acids; D004364:Pharmaceutical Preparations; D010743:Phospholipids; D003596:Cytosine