S179D prolactin diminishes the effects of UV light on epidermal gamma delta T cells.
Epidermal gamma delta T cells (gammadeltaT) and Langerhans cells (LC) are immune cells altered by exposure to ultraviolet radiation (UVB), a powerful stressor resulting in immune suppression. Prolactin (PRL) has been characterized as an immunomodulator, particularly during stress. In this study, we have asked whether separate administration of the 2 major forms of prolactin, unmodified and phosphorylated, to groups of 15 mice (3 experiments, each with 5 mice per treatment group) affected the number and morphology of these epidermal immune cells under control conditions, and following UV-irradiation. Under control conditions, both PRLs reduced the number of gammadeltaT, but a molecular mimic of phosphorylated PRL (S179D PRL) was more effective, resulting in a 30% reduction. In the irradiated group, however, S179D PRL was protective against a UV-induced reduction in gammadeltaT number and change in morphology (halved the reduction and normalized the morphology). In addition, S179D PRL, but not unmodified (U-PRL), maintained a normal LC:gammadeltaT ratio and sustained the dendritic morphology of LC after UV exposure. These findings suggest that S179D PRL may have an overall protective effect, countering UV-induced cellular alterations in the epidermis.
Guzmán
Esther A
EA
The University of Texas, Houston, Graduate School of Biomedical Sciences, Houston, TX 77030, United States.
Langowski
John L
JL
De Guzman
Ariel
A
Muller
H Konrad
HK
Walker
Ameae M
AM
Owen
Laurie B
LB
D000818:Animals; D004817:Epidermis / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D007155:Immunologic Factors / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D015918:Infusion Pumps, Implantable; D007801:Langerhans Cells / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010766:Phosphorylation; D011388:Prolactin / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
D007155:Immunologic Factors; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011994:Recombinant Proteins; C422171:prolactin, Asp(179)-; D011388:Prolactin