title: Isolation of CD24(high) and CD24(low/-) cells from MCF-7: CD24 expression is positively related with proliferation, adhesion and invasion in MCF-7.
abstract: CD24 has been reported to have a role in metastasis of several malignancies including breast cancer. We investigated the effect of CD24 expression on biologic features in human breast cancer cells, and found that CD24 expression is associated with more rapid growth and enhanced ability of adhesion and invasion in MCF-7. The proportion of cells was higher in synthetic phase and lower in arrestic phase for CD24(high) than for CD24(low/-) cells. Cyclin D1 and p27 had stronger expressions in CD24(low/-) cells than CD24(high) cells. These suggested one possible pathway for CD24 effect on proliferation.
authors.affiliation: Department of Surgery, Seoul National University College of Medicine, 28 Youngon-Dong, Chongon-Gu, Seoul, Republic of Korea. nirvana804@hanmail.net
authors.forename: Hee Joung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jong Bin
authors.initials: JB
authors.lastname: Kim
authors.forename: Kyung-Min
authors.initials: KM
authors.lastname: Lee
authors.forename: Incheol
authors.initials: I
authors.lastname: Shin
authors.forename: Wonshik
authors.initials: W
authors.lastname: Han
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Ko
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Bae
authors.forename: Dong-Young
authors.initials: DY
authors.lastname: Noh
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051927:CD24 Antigen / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D056741:Cyclin D; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D009361:Neoplasm Invasiveness / Q000473:pathology; D013629:Tamoxifen / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
chemical_list: D000903:Antibiotics, Antineoplastic; D018931:Antineoplastic Agents, Hormonal; D051927:CD24 Antigen; D056741:Cyclin D; D016213:Cyclins; D047628:Estrogen Receptor alpha; D013629:Tamoxifen; D050760:Cyclin-Dependent Kinase Inhibitor p27; D004317:Doxorubicin