title: Antioxidant capacity and in vitro prevention of dental plaque formation by extracts and condensed tannins of Paullinia cupana.
abstract: Chemical evaluation of the semi-purified fraction from the seeds of guaraná, Paullinia cupana H.B.K. var. sorbilis (Mart.) Ducke, yielded the following compounds: caffeine, catechin, epicatechin, ent-epicatechin, and procyanidins B1, B2, B3, B4, A2, and C1. Measurement of the antioxidant activity by reduction of the DPPH radical confirmed the anti-radical properties of the aqueous (AqE) and crude (EBPC) extracts and semi-purified (EPA and EPB) fractions. The EPA fraction showed radical-scavenging activity (RSA) and protected DPPH from discoloration at 5.23 +/- 0.08 (RSD% = 1.49) microg/mL, and for the phosphomolybdenum complex showed a higher Relative Antioxidant Capacity (RAC) at 0.75 +/- 0.01 (1.75). The EPA fraction had a total polyphenolics content of 65.80% +/- 0.62 (RSD% = 0.93). The plant drug showed 5.47% +/- 0.19 (RSD% = 3.51) and 6.19% +/- 0.08 (RSD% = 1.29) for total polyphenolics and methylxanthines, respectively. In vitro assessment of the antibacterial potential of the Paullinia cupana extracts against Streptococcus mutans showed that these could be used in the prevention of bacterial dental plaque.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá, Av. Colombo, 5790, BR-87020-900, Maringá, PR, Brazil.
authors.forename: Elza
authors.initials: E
authors.lastname: Yamaguti-Sasaki
authors.forename: Lia Akina
authors.initials: LA
authors.lastname: Ito
authors.forename: Vanessa Cristina Dias
authors.initials: VC
authors.lastname: Canteli
authors.forename: Tânia Mara Antonelli
authors.initials: TM
authors.lastname: Ushirobira
authors.forename: Tânia
authors.initials: T
authors.lastname: Ueda-Nakamura
authors.forename: Benedito Prado
authors.initials: BP
authors.lastname: Dias Filho
authors.forename: Celso Vataru
authors.initials: CV
authors.lastname: Nakamura
authors.forename: João Carlos Palazzo
authors.initials: JC
authors.lastname: de Mello
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D003773:Dental Plaque / Q000382:microbiology / Q000517:prevention & control*; D006801:Humans; D032126:Paullinia / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D013295:Streptococcus mutans / Q000187:drug effects*; D013634:Tannins / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D013634:Tannins