Spectrofluorimetric determination of dopamine using 1,5-bis(4,6-dicholro-1,3,5-triazinylamino) naphthalene.
A new fluorescent reagent, 1,5-bis(4,6-dichloro-1,3,5-triazinylamino)naphthalene, containing two active chlorines, was synthesized by a one-step reaction. Under the optimum conditions for the determination of dopamine, the enhanced fluorescence intensity is proportional to the dopamine concentration. The fluorescence intensity was measured at lambda(ex/em) = 400/460 nm, with and without dopamine. The linear range and detection limit for the determination of dopamine were 1.0 x 10(-7) mol/L-5.0 x 10(-5) mol/L and 4.0 x 10(-8) mol/L. This method is simple, practical, can afford good precision and accuracy and can be successfully applied to assess dopamine in injections and human serum samples.
Wei
Wei
W
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, People's Republic of China.
Wang
Hong-Jian
HJ
Jiang
Chong-Qiu
CQ
D015057:1-Naphthylamine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D004298:Dopamine / Q000097:blood*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014227:Triazines / Q000138:chemical synthesis*
C528187:1,5-bis(4,6-dichloro-1,3,5-triazinylamino)naphthalene; D005456:Fluorescent Dyes; D014227:Triazines; D015057:1-Naphthylamine; D004298:Dopamine