title: Analysis of -1082 IL-10 gene polymorphism in Iranian patients with generalized aggressive periodontitis.
abstract: BACKGROUND
  Periodontitis is a multifactorial disease and its severe forms, such as aggressive periodontitis, are suggested to have a genetic basis. Among the genetic factors, polymorphisms in cytokine genes have recently been described in susceptibility to periodontitis. IL-10 is a multi-functional cytokine thought to play a role in the pathogenesis of periodontitis. A substitution G/A polymorphism in the promoter region of the IL-10 gene at position -1082 has been associated with different amounts of IL-10 production. The aim of the present study was to investigate the possible links between -1082(G/A) polymorphism of the IL-10 gene and the generalized form of aggressive periodontitis.
  
  
  METHODS
  This study included 52 Iranian Khorasanian (north-east province of Iran) subjects suffering from generalized aggressive periodontitis referred to the Periodontology Department of Mashhad Dental School. They were compared to 61 age and sex-matched healthy controls of the same race. DNA was isolated from peripheral blood cells and genotyping was performed by means of the amplification refractory mutation system polymerase chain reaction (ARMS-PCR) method. Data were analyzed using the chi-squared test.
  
  
  RESULTS
  There was no marked difference in genotype frequencies between the controls and generalized aggressive periodontitis patients (p=0.585). Moreover, no association between patients and normal subjects was found in their allele frequency (p=0.329).
  
  
  CONCLUSIONS
  We conclude that the polymorphic nucleotide A at position -1082 of the IL-10 gene is not associated with generalized aggressive periodontitis in the Iranian population.
authors.affiliation: Department of Periodontology, School of Dentistry and Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Mellati
authors.forename: Hamid R
authors.initials: HR
authors.lastname: Arab
authors.forename: Jalil
authors.initials: J
authors.lastname: Tavakkol-Afshari
authors.forename: Ahmad R
authors.initials: AR
authors.lastname: Ebadian
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Radvar
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D001483:Base Sequence; D016022:Case-Control Studies; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D007492:Iran; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000235:genetics* / Q000276:immunology*; D020641:Polymorphism, Single Nucleotide
chemical_list: D017931:DNA Primers; C508609:IL10 protein, human; D016753:Interleukin-10