title: Serum deoxyribonuclease I activity can be used as a novel marker of transient myocardial ischaemia: results in vasospastic angina pectoris induced by provocation test.
abstract: OBJECTIVE
  Serum deoxyribonuclease I (DNase I) activity has recently been highlighted as a potential diagnostic marker for detection of acute myocardial infarction. To evaluate whether serum DNase I activity is useful for detection of myocardial ischaemia, we investigated alteration of its levels after onset of vasospastic angina pectoris (VSAP), resulting in transient myocardial ischaemia, induced by the intracoronary ergonovine provocation test.
  
  
  RESULTS
  Twenty-nine consecutive patients with suspected VSAP were subjected to the test. Patients were categorized as VSAP-positive (n = 13) or -negative (n = 16) based on development of angina. Serum samples were examined for DNase I activity before, immediately after, and 3, 6, and 24 h after the provocation tests. The serum DNase I activity increased significantly from the baseline 3 h after the provocation test in 11 patients of the VSAP-positive group whose levels of troponin T were within the normal range. Median of the percentage differences from the baseline in serum DNase I activity 3 h after the test was 32.1% (25th and 75th percentile: 28.6 and 42.0%, respectively; P = 0.000012). In the VSAP-negative group, levels of DNase I activity remained unchanged at any point of time after the provocation test.
  
  
  CONCLUSIONS
  Transient myocardial ischaemia resulting from VSAP induces a significant elevation of serum DNase I activity. Therefore, serum DNase I activity may be applicable as a useful marker for detecting transient myocardial ischaemia.
authors.affiliation: Third Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Eiheiji, Fukui, Japan.
authors.forename: Norihiro
authors.initials: N
authors.lastname: Morikawa
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kawai
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Arakawa
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Kumamoto
authors.forename: Isamu
authors.initials: I
authors.lastname: Miyamori
authors.forename: Hironobu
authors.initials: H
authors.lastname: Akao
authors.forename: Michihiko
authors.initials: M
authors.lastname: Kitayama
authors.forename: Kouji
authors.initials: K
authors.lastname: Kajinami
authors.forename: Jong-Dae
authors.initials: JD
authors.lastname: Lee
authors.forename: Haruo
authors.initials: H
authors.lastname: Takeshita
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Kominato
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Yasuda
mesh_terms: D000368:Aged; D000788:Angina Pectoris, Variant / Q000139:chemically induced / Q000175:diagnosis* / Q000000981:diagnostic imaging; D015415:Biomarkers / Q000097:blood; D006328:Cardiac Catheterization / Q000379:methods; D017023:Coronary Angiography / Q000379:methods; D003850:Deoxyribonuclease I / Q000097:blood*; D004874:Ergonovine; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000139:chemically induced / Q000175:diagnosis* / Q000000981:diagnostic imaging; D010120:Oxytocics
chemical_list: D015415:Biomarkers; D010120:Oxytocics; D003850:Deoxyribonuclease I; D004874:Ergonovine