Localization of proteins and organelles using fluorescence microscopy.
This chapter describes the different methods used for localization of proteins and organelles in Pichia pastoris. A series of plasmids and a modified immunofluorescence protocol for localization and co-localization of proteins and organelles are described. Also included are protocols for the labeling of different subcellular organelles with vital stains.
Farre
Jean-Claude
JC
Section of Molecular Biology, Division of Biological Sciences, University of California San Diego, La Jolla, CA, USA.
Shirahama-Noda
Kanae
K
Zhang
Lan
L
Booher
Keith
K
Subramani
Suresh
S
D002467:Cell Nucleus / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007211:Indoles; D050296:Microbial Viability; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism; D015388:Organelles / Q000378:metabolism*; D010843:Pichia / Q000166:cytology* / Q000378:metabolism*; D021381:Protein Transport; D011506:Proteins / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism
D007211:Indoles; D011506:Proteins; C007293:DAPI