Endothelial dysfunction and oxidative stress in type 1 and type 2 diabetic patients without clinical macrovascular complications.
OBJECTIVE
To evaluate the relationship between oxidative stress and endothelial dysfunction (ED) in diabetic patients without clinical macrovascular complications.


METHODS
In 27 type 1, 56 type 2 diabetic patients and 35 healthy controls the redox state (GSH, GSSG; enzymatic method), endothelin-1 (ET-1; ELISA) and von Willebrand factor (vWF; ELISA) plasma levels, urinary vascular endothelial growth factor (VEGF; ELISA) were measured.


RESULTS
Decreased GSH levels (p<0.05, type 1 and type 2), GSH/GSSG ratio (p<0.05 type 1, p<0.001 type 2) and elevated vWF levels (p<0.001, type 1 and type 2) were observed in diabetic patients in comparison with controls. A negative correlation between GSH and vWF (p<0.02 and p<0.001, in type 1 and type 2, respectively) and GSH and BMI (p<0.02 in type 1 and type 2) was observed. ET-1 was positively correlated to age (p<0.05) and diabetes duration (p<0.03) in type 1, while vWF was correlated to systolic blood pressure (p<0.05) in type 2 diabetic patients. Urinary VEGF was higher in type 2 (p<0.05) in comparison with type 1 diabetic patients and was correlated to glycemia (p<0.05) and systolic blood pressure (p<0.05).


CONCLUSIONS
These data might indicate that markers of oxidative stress and ED are altered in diabetic patients without clinical macrovascular complications.
De Mattia
Giancarlo
G
Department of Internal Medicine, Policlinico Umberto I, Sapienza University, Viale del Policlinico 155, 00161 Rome, Italy.
Bravi
Maria Cristina
MC
Laurenti
Oriana
O
Moretti
Arianna
A
Cipriani
Rosalba
R
Gatti
Alessandra
A
Mandosi
Elisabetta
E
Morano
Susanna
S
D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000652:urine; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000503:physiopathology* / Q000652:urine; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000503:physiopathology* / Q000652:urine; D003930:Diabetic Retinopathy / Q000453:epidemiology; D019332:Endothelin-1 / Q000097:blood; D004730:Endothelium, Vascular / Q000503:physiopathology*; D005260:Female; D005978:Glutathione / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012016:Reference Values; D042461:Vascular Endothelial Growth Factor A / Q000652:urine; D014841:von Willebrand Factor / Q000378:metabolism
D015415:Biomarkers; D019332:Endothelin-1; D042461:Vascular Endothelial Growth Factor A; D014841:von Willebrand Factor; D005978:Glutathione