title: Genes involved in the RNA interference pathway are differentially expressed during sea urchin development.
abstract: RNA-mediated interference (RNAi) is a conserved gene silencing mechanism that involves double-stranded RNA as a signal to trigger the sequence-specific degradation of target mRNA, resulting in posttranscriptional silencing and/or translational repression. Bioinformatic searches in the sea urchin genome database identified homologs of Drosha, DGCR5, Dicer, TRBP, Exportin-5, and Argonautes. Quantitative, real-time polymerase chain reaction indicated that all mRNA accumulate in eggs and in variable levels throughout early development. Whole-mount in situ RNA hybridization showed that all of the important players of the RNAi silencing pathway have abundant mRNA accumulation in oocytes and eggs, but have distinct spatial and temporal expression patterns throughout development. Sequence analysis revealed that each of the four Argonautes examined contain conserved residues important for RNAseH activity within the Piwi domain. This study elucidated that genes involved in the RNAi silencing pathway have dynamic expression and, thus, may have regulatory roles during germ cell development and embryogenesis.
authors.affiliation: Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA. jia_song@brown.edu
authors.forename: Jia L
authors.initials: JL
authors.lastname: Song
authors.forename: Gary M
authors.initials: GM
authors.lastname: Wessel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D010053:Ovary / Q000196:embryology / Q000378:metabolism; D010802:Phylogeny; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D047270:Strongylocentrotus purpuratus / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism
chemical_list: D011506:Proteins; D012333:RNA, Messenger