title: Brucellosis of the lung: case report and review of the literature.
abstract: Human brucellosis is a worldwide re-emerging zoonosis. However, its histological appearance has only been occasionally described. We report the case of a young girl who had been suffering from a spontaneous fracture of the eighth thoracic vertebra at the age of 7. At the age of 15, X-ray showed a translucence of the seventh and ninth thoracic vertebra, and additionally, a bi-lateral episcleritis was detected. Three months later, she was admitted to the hospital because of perspiration at night and moderate fever. Computer tomography revealed coarsely spotted infiltrates in the lower fields of both lungs. Serology for rheumatic diseases was negative. Thoracoscopical wedge resection was done for histological clarification of pulmonary changes. Microscopically, a granulomatous inflammation with central necrosis was seen. A Ziehl-Neelsen stain did not demonstrate acid-fast bacteria. In spite of negative serology, real-time polymerase chain reaction detected Brucella melitensis deoxyribonucleic acid in the formalin-fixed tissue samples of the lung. Interrogation of the patient revealed visits in different Arabian countries during childhood as a presumable source of infection. In conclusion, granulomatous inflammation negative for Ziehl-Neelsen and Grocott stains presenting together with other localized lesions should lead to specific investigations on brucellosis.
authors.affiliation: Institute of Pathology and Neuropathology, University Hospital Essen, University of Duisburg-Essen, Hufelandstrasse 55, Essen, Germany. dirk.theegarten@uk-essen.de
authors.forename: Dirk
authors.initials: D
authors.lastname: Theegarten
authors.forename: Sven
authors.initials: S
authors.lastname: Albrecht
authors.forename: Martin
authors.initials: M
authors.lastname: Tötsch
authors.forename: Helmut
authors.initials: H
authors.lastname: Teschler
authors.forename: Heinrich
authors.initials: H
authors.lastname: Neubauer
authors.forename: Sascha
authors.initials: S
authors.lastname: Al Dahouk
mesh_terms: D000293:Adolescent; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D017347:Brucella melitensis / Q000235:genetics / Q000302:isolation & purification*; D002006:Brucellosis / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D003937:Diagnosis, Differential; D004318:Doxycycline / Q000627:therapeutic use; D004359:Drug Therapy, Combination; D005260:Female; D006801:Humans; D008171:Lung Diseases / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology; D008279:Magnetic Resonance Imaging; D011241:Prednisone / Q000627:therapeutic use; D013902:Radiography, Thoracic; D012293:Rifampin / Q000627:therapeutic use; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004318:Doxycycline; D011241:Prednisone; D012293:Rifampin