title: Quantitative small animal fluorescence tomography using an ultrafast gated image intensifier.
abstract: Optical approaches to small animal in vivo molecular imaging provide high sensitivity, stable non-radioactive probes, and an extensive array of functional reporting strategies. However, quantitative whole body assays remain illusive. The quantitative accuracy of optical imaging is affected by the depth of the buried target and the heterogeneity of tissue optical properties. Tomography approaches, to obtaining in-vivo optical property maps, and whole body distributions of fluorescing probes, provide a strategy for improving the quality and quantitative accuracy of small animal optical imaging. Here we present a time-resolved, charged coupled device (CCD) based system for quantitative small animal fluorescence tomography.
authors.affiliation: Dept. of Radiol., Washington Univ. Sch. of Med., St. Louis, MO 63110, USA. patwardhans@mir.wustl.edu
authors.forename: Sachin V
authors.initials: SV
authors.lastname: Patwardhan
authors.forename: Sharon
authors.initials: S
authors.lastname: Bloch
authors.forename: Samuel
authors.initials: S
authors.lastname: Achilefu
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Culver
mesh_terms: D000818:Animals; D003626:Data Display; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000662:veterinary*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041622:Tomography, Optical / Q000295:instrumentation* / Q000662:veterinary*; D051598:Whole Body Imaging / Q000295:instrumentation* / Q000662:veterinary*