A monoclonal antibody for G protein-coupled receptor crystallography.
G protein-coupled receptors (GPCRs) constitute the largest family of signaling proteins in mammals, mediating responses to hormones, neurotransmitters, and senses of sight, smell and taste. Mechanistic insight into GPCR signal transduction is limited by a paucity of high-resolution structural information. We describe the generation of a monoclonal antibody that recognizes the third intracellular loop (IL3) of the native human beta(2) adrenergic (beta(2)AR) receptor; this antibody was critical for acquiring diffraction-quality crystals.
Day
Peter W
PW
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, 279 Campus Drive, Stanford, Palo Alto, California 94305, USA.
Rasmussen
Søren G F
SG
Parnot
Charles
C
Fung
Juan José
JJ
Masood
Asna
A
Kobilka
Tong Sun
TS
Yao
Xiao-Jie
XJ
Choi
Hee-Jung
HJ
Weis
William I
WI
Rohrer
Daniel K
DK
Kobilka
Brian K
BK
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000937:Antigen-Antibody Reactions / Q000276:immunology; D015153:Blotting, Western; D003460:Crystallization / Q000379:methods; D003461:Crystallography; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D018343:Receptors, Adrenergic, beta-2 / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D012235:Rhodamines / Q000737:chemistry; D014611:Vaccination
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005456:Fluorescent Dyes; D007140:Immunoglobulin Fab Fragments; D018343:Receptors, Adrenergic, beta-2; D043562:Receptors, G-Protein-Coupled; D011994:Recombinant Proteins; D012235:Rhodamines; C005358:tetramethylrhodamine