Novel flutamide regulated genes in the rat ventral prostate: differential modulation of their expression by castration and flutamide treatments.
OBJECTIVE
To identify flutamide regulated genes in the rat ventral prostate.


METHODS
Total RNA from ventral prostates of control and flutamide treated rats were isolated. Differentially expressed transcripts were identified using differential display reverse transcriptase polymerase chain reaction. The effect of castration on the expression of flutamide-regulated transcripts was studied.


RESULTS
We have identified beta2-microglobulin, cytoplasmic FMR1 interacting protein 2 and pumilio 1 as flutamide induced and spermine binding protein and ribophorin II as flutamide repressed targets in the rat ventral prostate. Although flutamide treatment caused an induction of pumilio 1 mRNA, castration had no effect.


CONCLUSIONS
Castration and flutamide treatments exert differential effects on gene expression. Flutamide might also have direct AR independent effects, which might have implications in the emergence of androgen independent prostate cancer and the failure of flutamide therapy.
Limaye
Anil M
AM
Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, India.
Asangani
Irfan
I
Bora
Namrata
N
Kondaiah
Paturu
P
D048868:Adaptor Proteins, Signal Transducing / Q000187:drug effects / Q000378:metabolism; D000726:Androgen Antagonists / Q000494:pharmacology*; D000818:Animals; D005485:Flutamide / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009919:Orchiectomy; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D001613:beta 2-Microglobulin / Q000187:drug effects / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D000726:Androgen Antagonists; C525634:Cyfip2 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C525635:Pum1 protein, rat; D016601:RNA-Binding Proteins; C032603:Sbp protein, rat; D001613:beta 2-Microglobulin; C034241:ribophorin; D005485:Flutamide