Use of sera from humans and dolphins with lacaziosis and sera from experimentally infected mice for Western Blot analyses of Lacazia loboi antigens.
Antibodies in the sera of patients with lacaziosis recognized an approximately 193-kDa antigen and other Lacazia loboi antigens. Paracoccidioides brasiliensis gp43 antigen was detected by all evaluated sera, but they failed to detect a protein with the same molecular mass in L. loboi extracts. This study is the first to examine the humoral response to L. loboi antigens by using multiple host sera.
Mendoza
Leonel
L
Biomedical Laboratory Diagnostics Program, Michigan State University, 322 North Kedzie Hall, East Lansing, MI 48824-1031, USA. mendoza9@msu.edu
Belone
Andréa F F
AF
Vilela
Raquel
R
Rehtanz
Manuela
M
Bossart
Gregory D
GD
Reif
John S
JS
Fair
Patricia A
PA
Durden
Wendy N
WN
St Leger
Judy
J
Travassos
Luiz R
LR
Rosa
Patricia S
PS
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology / Q000302:isolation & purification; D000946:Antigens, Fungal / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification; D015153:Blotting, Western / Q000379:methods*; D050681:Bottle-Nosed Dolphin; D005656:Fungal Proteins / Q000097:blood / Q000276:immunology; D006023:Glycoproteins / Q000097:blood / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D009181:Mycoses / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D020036:Onygenales / Q000276:immunology* / Q000302:isolation & purification; D010228:Paracoccidioides / Q000276:immunology / Q000302:isolation & purification; D010229:Paracoccidioidomycosis / Q000097:blood / Q000175:diagnosis / Q000382:microbiology
C103180:43 kDa protein, Paracoccidioides; D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D005656:Fungal Proteins; D006023:Glycoproteins; D007074:Immunoglobulin G