[Study on the effect of electric field on the secondary structure of SOD by FTIR spectroscopy].
After the SOD (superoxide dismutase) was treated with different strength of electric field, the effect of electric field on the secondary structure of SOD was studied by FTIR (Fourier-transformation infrared spectroscopy). The results have shown that different electric field strength has different effects on the secondary structure contents of the SOD. As compared with the contrast, the relative contents of alpha-helix and beta-sheet were decreased, while beta-turn content increased. The random coil contents were increased except for the 1.0 kV x cm(-1). The electric treatment tends to transform the alpha-helix and beta-sheet into beta-turn and random coil, and different strength of electric field has a different effect on the transformation. The changes in SOD activity have a direct relationship to the contents of beta-sheet.
Deng
Yi-Bing
YB
Marine Science College of Zhejiang Ocean University, Zhoushan 316004, China. dyb658@126.com
Yang
Ti-Qiang
TQ