title: Regulation of the interferon-inducible p53 target gene TRIM22 (Staf50) in human T lymphocyte activation.
abstract: TRIM22 (Staf50) is an interferon (IFN)-inducible protein with unknown function. Recently, we identified TRIM22 as a novel p53 target gene and showed that overexpression of TRIM22 inhibits the clonogenic growth of monoblastic U937 cells. Moreover, expression of TRIM22 is high in lymphoid tissue, and levels decrease during T lymphocyte activation with CD3/CD2/CD28, suggesting that TRIM22 could exert antiproliferative effects. Here, a prominent increase in TRIM22 levels is observed during activation with interleukin-2 (IL-2) or IL-15 in contrast to the decrease observed during CD3/CD2/CD28-induced activation. However, stimulation of cells in these experiments was performed on crude T lymphocytes, allowing indirect regulation between different lymphocyte subtypes to take place. Therefore, to prevent interaction between different lymphocyte subtypes, expression of TRIM22 was examined during activation of sorted T lymphocyte subpopulations. In contrast to the marked changes of TRIM22 during activation of crude T lymphocytes, in isolated subpopulations, TRIM22 expression was not significantly affected in spite of IL-2-induced or CD3/CD2/CD28-induced activation. In addition, in contrast to the TRIM22 mouse ortholog Rpt-1, TRIM22 did not affect levels of CD25 (IL-2Ralpha) mRNA. Our data suggest a more complex role for TRIM22 during T lymphocyte activation than merely as an antiproliferative factor. TRIM22 probably has an activation stage-specific role connected to the paracrine crosstalk during T lymphocyte activation.
authors.affiliation: Department of Haematology and Transfusion Medicine, Lund University, S-221 84 Lund, Sweden. Susanna.Obad@med.lu.se
authors.forename: Susanna
authors.initials: S
authors.lastname: Obad
authors.forename: Tor
authors.initials: T
authors.lastname: Olofsson
authors.forename: Nadir
authors.initials: N
authors.lastname: Mechti
authors.forename: Urban
authors.initials: U
authors.lastname: Gullberg
authors.forename: Kristina
authors.initials: K
authors.lastname: Drott
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D018801:CD2 Antigens / Q000276:immunology; D018106:CD28 Antigens / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D019409:Interleukin-15 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D019169:Jurkat Cells; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D015778:Minor Histocompatibility Antigens; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000070584:Tripartite Motif Proteins; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D000906:Antibodies; D018801:CD2 Antigens; D018106:CD28 Antigens; D017252:CD3 Complex; D004268:DNA-Binding Proteins; D019409:Interleukin-15; D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit; D015778:Minor Histocompatibility Antigens; D012333:RNA, Messenger; D012097:Repressor Proteins; C094314:TRIM22 protein, human; D014157:Transcription Factors; C055548:Trim30a protein, mouse; D000070584:Tripartite Motif Proteins; D016159:Tumor Suppressor Protein p53; D007372:Interferons