Cell encapsulating droplet vitrification.
The capability to encapsulate single cells in droplets while retaining high cell viability (>90%) has great impact on tissue engineering, high-throughput screening, as well as clinical diagnostics and therapeutics. We demonstrate a novel method to vitrify a small number of cells using cell-encapsulating droplets. The method allows vitrification at low cryoprotectant concentration (1.5 M propanediol and 0.5 M trehalose), similar to that used in slow freezing protocols. The method was successfully applied to five different mammalian cell types: AML-12 hepatocytes, NIH-3T3 fibroblasts, HL-1 cardiomyocytes, mouse embryonic stem cells, and RAJI cells.
Demirci
Utkan
U
Harvard-Massachusetts Institute of Technology Health Sciences and Technology, Cambridge, MA, USA. udemirci@rics.bwh.harvard.edu
Montesano
Grace
G
D002470:Cell Survival; D002477:Cells