[Comparative study on regeneration systems of Cunninghamia lanceolata hook].
Four regeneration systems derived from cotyledon, hypocotyls, stem and needle explants have been established in the comparative researches of regeneration systems in Cunninghamia lanceolata Hook. A high frequency (93.7% +/- 0.45%) of adventitious buds were induced from cotyledons on DCR medium supplemented with 1 mg/L BA and 0.1 mg/L NAA, with a maximum mean number buds of 3.76 +/- 0.25. A higher frequency (96.2% +/- 0.35%) of adventitious buds were induced from hypocotyls on DCR medium supplemented with 2 mg/L BA and 0.2 mg/L NAA, with a maximum mean number buds of 17.4 +/- 0.18. A highest frequency (100%) of adventitious buds were induced from stem segments on DCR medium supplemented with 1 mg/L BA and 0.1 mg/L NAA, with a maximum mean number buds of 3.28 +/- 0.11. A low frequency (84.5% +/- 0.45%) of adventitious buds were induced from needles on DCR medium supplemented with 1.5 mg/L BA and 0.1 mg/L NAA, with a maximum mean number buds of 1.42 +/- 0.08. Buds were elongated on DCR medium supplemented with 0.2 mg/L BA and 0.02 mg/L NAA. After pre-treating shoots, best rooting result was produced on 1/2 DCR medium supplemented with 0.3 mg/L IBA.
Zhu
Mu Lan
ML
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Science, Shanghai 200032.
Wang
Ji
J
Wei
Zhi Ming
ZM
D018548:Cotyledon / Q000502:physiology; D032486:Cunninghamia / Q000502:physiology*; D018546:Hypocotyl / Q000502:physiology; D018547:Plant Stems / Q000502:physiology; D018514:Plant Structures / Q000502:physiology; D012038:Regeneration / Q000502:physiology*; D046509:Tissue Culture Techniques