Quantitative real-time PCR detection of adenovirus in clinical blood specimens: a comparison of plasma, whole blood and peripheral blood mononuclear cells.
BACKGROUND
Detection and quantification of adenovirus (ADV) in peripheral blood specimens has become an increasingly important tool in the management of immunosuppressed patients. Investigators have described the use of whole blood (WB), peripheral blood mononuclear cells (PBMC), serum and plasma but no studies have compared the utility of these different sample types for use in a clinical diagnostic assay.


OBJECTIVE
To determine the optimal blood compartment for quantitative real-time measurement of adenovirus in peripheral blood specimens.


METHODS
WB, PBMC, and plasma representing 338 samples from 148 patients were tested for ADV by quantitative real-time PCR (qrt-PCR) and the results compared for concordance of both qualitative sensitivity and viral load among positive specimens.


RESULTS
There was no significant difference in qualitative sensitivity among the three tested specimen types. Quantitative values of WB and plasma were similar and tended to be greater than those found in PBMC samples. Comparison of consecutive positive samples within individual patients showed that viral loads tracked similarly over time, irrespective of the sample type tested.


CONCLUSIONS
While WB and plasma do not offer a significant increase in sensitivity over PBMC, they may offer benefits in terms of reduced processing costs and laboratory turn around time.
Perlman
J
J
Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, United States.
Gibson
C
C
Pounds
S B
SB
Gu
Z
Z
Bankowski
M J
MJ
Hayden
R T
RT
D000256:Adenoviridae / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000097:blood* / Q000276:immunology / Q000821:virology*; D006801:Humans; D016867:Immunocompromised Host; D007963:Leukocytes, Mononuclear / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D019562:Viral Load / Q000379:methods