Plant pathogen recognition mediated by promoter activation of the pepper Bs3 resistance gene.
Plant disease resistance (R) proteins recognize matching pathogen avirulence proteins. Alleles of the pepper R gene Bs3 mediate recognition of the Xanthomonas campestris pv. vesicatoria (Xcv) type III effector protein AvrBs3 and its deletion derivative AvrBs3Deltarep16. Pepper Bs3 and its allelic variant Bs3-E encode flavin monooxygenases with a previously unknown structure and are transcriptionally activated by the Xcv effector proteins AvrBs3 and AvrBs3Deltarep16, respectively. We found that recognition specificity resides in the Bs3 and Bs3-E promoters and is determined by binding of AvrBs3 or AvrBs3Deltarep16 to a defined promoter region. Our data suggest a recognition mechanism in which the Avr protein binds and activates the promoter of the cognate R gene.
Römer
Patrick
P
Institute of Biology, Department of Genetics, Martin-Luther-University Halle-Wittenberg, D-06099 Halle (Saale), Germany.
Hahn
Simone
S
Jordan
Tina
T
Strauss
Tina
T
Bonas
Ulla
U
Lahaye
Thomas
T
D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002212:Capsicum / Q000235:genetics* / Q000382:microbiology*; D022202:Chromosomes, Artificial, Bacterial; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000235:genetics; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D016959:Xanthomonas campestris / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*
D001426:Bacterial Proteins; D010940:Plant Proteins; D006899:Mixed Function Oxygenases