[Selection of attenuated Vibrio cholerae strains to obtain oral attenuated candidate vaccines against cholera].
A methodology was developed for the selection of genetically modified strains of Vibrio cholerae 01 and 0139 aimed at obtaining oral attenuated candidate vaccines against cholera. The modified strains underwent microbiological characterization, bacterial susceptibility and different biological tests (mean lethal dose, colonizing capacity, adherence in mice, ligated intestine and intraduodenal inoculation in rabbits as virulence and potency tests. The strains 81, 638, 638T and 1333 were evaluated in clinical trials to determine their reactogenicity and immunogenicity. All the strains were sensitive to tetracycline and doxoclycine. They showed their attenuation and immunogenicity in animal models. The strains 638 and 1333 proved to be immunogenic and non reactogenic in volunteers.
María García
Hilda
H
Instituto Finlay, Ciudad de La Habana, Cuba. hgarcia@finlay.edu.cu
Año
Gemma
G
Cedré
Bárbara
B
Valmaseda
Tania
T
Maestre
Jorge Luis
JL
Díaz
Manuel
M
Mirabal
Mayelin
M
Talavera
Arturo
A
García
Luis
L
D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D002771:Cholera / Q000517:prevention & control*; D022121:Cholera Vaccines / Q000008:administration & dosage / Q000009:adverse effects / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D003627:Data Interpretation, Statistical; D004318:Doxycycline / Q000494:pharmacology; D006801:Humans; D051379:Mice; D046529:Microscopy, Electron, Transmission; D023421:Models, Animal; D011817:Rabbits; D013752:Tetracycline / Q000494:pharmacology; D014613:Vaccines, Attenuated; D014734:Vibrio cholerae / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000648:ultrastructure
D022121:Cholera Vaccines; D014613:Vaccines, Attenuated; D013752:Tetracycline; D004318:Doxycycline