title: Kupffer cells are central in the removal of nanoparticles from the organism.
abstract: BACKGROUND
  The study aims at revealing the fate of nanoparticles administered intravenously and intraperitoneally to adult female mice, some of which were pregnant. Gold nanoparticles were chosen as a model because these particles have been found to be chemically inert and at the same time are easily traced by autometallography (AMG) at both ultrastructural and light microscopic levels.
  
  
  RESULTS
  Gold nanoparticles were injected intravenously (IV) or intraperitoneally (IP) and traced after 1, 4 or 24 hours. For IV injections 2 and 40 nm particles were used; for IP injections 40 nm particles only. The injected nanoparticles were found in macrophages only, and at moderate exposure primarily in the Kupffer cells in the liver. IV injections resulted in a rapid accumulation/clustering of nanoparticles in these liver macrophages, while the uptake in spleen macrophages was moderate. IP injections were followed by a delayed uptake in the liver and included a moderate uptake in macrophages located in mesenteric lymph nodes, spleen and small intestine. Ultrastructurally, the AMG silver enhanced nanocrystals were found in lysosome-like organelles of the Kupffer cells and other macrophages wherever located.Accumulations of gold nanoparticles were not found in any other organs analysed, i.e. kidneys, brain, lungs, adrenals, ovaries, placenta, and fetal liver, and the control animals were all void of AMG staining.
  
  
  CONCLUSIONS
  Our results suggest that: (1) inert gold nanoparticles do not penetrate cell membranes by non-endocytotic mechanisms, but are rather taken up by endocytosis; (2) gold nanoparticles, independent of size, are taken up primarily by Kupffer cells in the liver and secondarily by macrophages in other places; (3) gold nanoparticles do not seem to penetrate the placenta barrier; (4) the blood-brain barrier seems to protect the central nervous system from gold nanoparticles; (5) 2 nanometer gold particles seem to be removed not only by endocytosis by macrophages, and we hypothesize that part of these tiny nanoparticles are released into the urine as a result of simple filtration in the renal glomeruli.
authors.affiliation: Department of Neurobiology, Institute of Anatomy, University of Aarhus, Building 1233/1234, Wilhelm Meyers Allé, DK-8000 Aarhus C, Denmark. gd@neuro.au.dk.
authors.forename: Evaldas
authors.initials: E
authors.lastname: Sadauskas
authors.forename: Håkan
authors.initials: H
authors.lastname: Wallin
authors.forename: Meredin
authors.initials: M
authors.lastname: Stoltenberg
authors.forename: Ulla
authors.initials: U
authors.lastname: Vogel
authors.forename: Peter
authors.initials: P
authors.lastname: Doering
authors.forename: Agnete
authors.initials: A
authors.lastname: Larsen
authors.forename: Gorm
authors.initials: G
authors.lastname: Danscher