title: Determination of eperisone in human plasma by liquid chromatography-ESI-tandem mass spectrometry.
abstract: A sensitive and selective method for the determination of 4'-ethyl-3-methyl-3-piperidinopropiophenone hydrochloride (eperisone hydrochloride) in human plasma was developed and validated. The procedure employed an internal standard and a solvent extraction step followed by chromatography on a Xterra C18 minibore column. Detection was by electrospray ionization tandem mass spectrometry with multiple reaction monitoring. The mass transitions of eperisone and tolperisone (IS) were m/z 260 --> 98 and m/z 246 --> 98, respectively. The method has a limit of detection of 0.1 pg/mL for eperisone based on the three times signal-to-noise value with a linear range from 0.01 to 10.0 ng/mL for the analyte. Extraction recovery was on average 98.6 +/- 7.2% (SD) for eperisone. The Intra- and inter-day assay accuracy ranged from 93 to 114% and precision (RSD) was better than 8.5%. The method was successfully employed to analyze plasma samples and evaluate pharmacokinetics of eperisone in healthy male volunteers.
authors.affiliation: College of Pharmacy, CBITRC, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Min Kyo
authors.initials: MK
authors.lastname: Jeoung
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Jeong
authors.forename: Nam Hee
authors.initials: NH
authors.lastname: Kim
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Kim
authors.forename: Youn-Bok
authors.initials: YB
authors.lastname: Chung
authors.forename: Yong-Moon
authors.initials: YM
authors.lastname: Lee
authors.forename: Su-Youn
authors.initials: SY
authors.lastname: Ahn
authors.forename: Hwang-Eui
authors.initials: HE
authors.lastname: Cho
authors.forename: Yong Hwa
authors.initials: YH
authors.lastname: Lee
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.forename: Dong-Cheul
authors.initials: DC
authors.lastname: Moon
mesh_terms: D000328:Adult; D019540:Area Under Curve; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D011427:Propiophenones / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D011427:Propiophenones; C030848:eperisone