title: A monoclonal antibody for G protein-coupled receptor crystallography.
abstract: G protein-coupled receptors (GPCRs) constitute the largest family of signaling proteins in mammals, mediating responses to hormones, neurotransmitters, and senses of sight, smell and taste. Mechanistic insight into GPCR signal transduction is limited by a paucity of high-resolution structural information. We describe the generation of a monoclonal antibody that recognizes the third intracellular loop (IL3) of the native human beta(2) adrenergic (beta(2)AR) receptor; this antibody was critical for acquiring diffraction-quality crystals.
authors.affiliation: Department of Molecular and Cellular Physiology, Stanford University School of Medicine, 279 Campus Drive, Stanford, Palo Alto, California 94305, USA.
authors.forename: Peter W
authors.initials: PW
authors.lastname: Day
authors.forename: Søren G F
authors.initials: SG
authors.lastname: Rasmussen
authors.forename: Charles
authors.initials: C
authors.lastname: Parnot
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Fung
authors.forename: Asna
authors.initials: A
authors.lastname: Masood
authors.forename: Tong Sun
authors.initials: TS
authors.lastname: Kobilka
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Yao
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Choi
authors.forename: William I
authors.initials: WI
authors.lastname: Weis
authors.forename: Daniel K
authors.initials: DK
authors.lastname: Rohrer
authors.forename: Brian K
authors.initials: BK
authors.lastname: Kobilka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000937:Antigen-Antibody Reactions / Q000276:immunology; D015153:Blotting, Western; D003460:Crystallization / Q000379:methods; D003461:Crystallography; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D018343:Receptors, Adrenergic, beta-2 / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D012235:Rhodamines / Q000737:chemistry; D014611:Vaccination
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D005456:Fluorescent Dyes; D007140:Immunoglobulin Fab Fragments; D018343:Receptors, Adrenergic, beta-2; D043562:Receptors, G-Protein-Coupled; D011994:Recombinant Proteins; D012235:Rhodamines; C005358:tetramethylrhodamine