title: Bioremediation of diesel fuel contaminated soil: effect of non ionic surfactants and selected bacteria addition.
abstract: Aim of this work was to evaluate influence of two commercial surfactants and inoculum of selected bacteria on biodegradation of diesel fuel in different systems. Among alkyl polyethossilates (Brij family) and sorbitan derivates (Tween family) a first selection of surfactants was performed by estimation of Koc and Dafnia magna EC50 with molecular descriptor and QSAR model. Further experiments were conducted to evaluate soil sorption, biodegradability and toxicity. In the second part of the research, the effect of Brij 56, Tween 80 and selected bacteria addition on biodegradation of diesel fuel was studied in liquid cultures and in slurry and solid phase systems. The latter experiments were performed with diesel contaminated soil in bench scale slurry phase bioreactor and solid phase columns. Tween 80 addition increased the biodegradation rate of hydrocarbons both in liquid and in slurry phase systems. Regarding the effect of inoculum, no enhancement of biodegradation rate was observed neither in surfactant added nor in experiments without addition. On the contrary, in solid phase experiments, inoculum addition resulted in enhanced biodegradation compared to surfactant addition.
authors.affiliation: Dept. Environmental Sciences, University of Milano-Bicocca, piazza della Scienza 1, 20126 Milano, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Collina
authors.forename: Marina
authors.initials: M
authors.lastname: Lasagni
authors.forename: Demetrio
authors.initials: D
authors.lastname: Pitea
authors.forename: Andrea
authors.initials: A
authors.lastname: Franzetti
authors.forename: Patrizia
authors.initials: P
authors.lastname: Di Gennaro
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Bestetti
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D052918:Environmental Restoration and Remediation / Q000379:methods*; D005742:Gasoline; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D005742:Gasoline; D012989:Soil Pollutants; D013501:Surface-Active Agents