title: A quantitative and qualitative method for direct 2-DE analysis of murine cartilage.
abstract: Direct 2-DE analysis of cartilage is difficult due to the high proteoglycan content. Proteoglycan removal before IEF may however cause the partial or total loss of specific proteins making this approach ineffective when quantitative data are required to investigate protein expression differences. Thus, we have developed a 2-DE method including passive rehydration loading that does not require sample pretreatment and allows direct protein expression studies in cartilage samples.
authors.affiliation: Dipartimento di Biochimica Alessandro Castellani, Università di Pavia, Pavia, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Pecora
authors.forename: Antonella
authors.initials: A
authors.lastname: Forlino
authors.forename: Benedetta
authors.initials: B
authors.lastname: Gualeni
authors.forename: Anna
authors.initials: A
authors.lastname: Lupi
authors.forename: Sofia
authors.initials: S
authors.lastname: Giorgetti
authors.forename: Loredana
authors.initials: L
authors.lastname: Marchese
authors.forename: Monica
authors.initials: M
authors.lastname: Stoppini
authors.forename: Ruggero
authors.initials: R
authors.lastname: Tenni
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cetta
authors.forename: Antonio
authors.initials: A
authors.lastname: Rossi
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002358:Cartilage, Articular / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D040081:Protein Array Analysis; D020543:Proteome / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D020543:Proteome