title: [Inhibitory effect of polysaccharides on the six-alpha-helix bundle formation of HIV gp41 protein].
abstract: OBJECTIVE
  To compare the in vitro inhibitory effect of expolysaccharides from Streptomyces, polysaccharides of Ganoderma lucidum and rice bran on six-alpha-helix bundle formation of HIV gp41 protein.
  
  
  METHODS
  The amount of six-alpha-helix bundle formed in the presence of N36 and C34 was tested by ELISA in response to treatments with different doses of polysaccharides.
  
  
  RESULTS
  Expolysaccharides from Streptomyces potentially inhibited six-alpha-helix bundle formation with the effective concentration (IC(50)) of 145.48-/+7.25 mg /L. Polysaccharides of Ganoderma lucidum and rice bran showed no effect on the six-alpha-helix bundle formation.
  
  
  CONCLUSIONS
  Expolysaccharides from Streptomyces can inhibit the six-alpha-helix bundle formation of HIV gp41, whereas polysaccharides of Ganoderma lucidum and rice bran do not exhibit such activity.
authors.affiliation: School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. wenxy@fimmu.com
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Wen
authors.forename: Shao-Yu
authors.initials: SY
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Li
authors.initials: L
authors.lastname: Lv
authors.forename: Shu-Wen
authors.initials: SW
authors.lastname: Liu
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Rao
authors.forename: Jia-Jie
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Guang-Fa
authors.initials: GF
authors.lastname: Wang
authors.forename: Shan-He
authors.initials: SH
authors.lastname: Wan
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Wu
mesh_terms: D015700:HIV Envelope Protein gp41 / Q000737:chemistry*; D007700:Kinetics; D012275:Oryza / Q000737:chemistry; D011134:Polysaccharides / Q000494:pharmacology*; D017433:Protein Structure, Secondary / Q000187:drug effects; D020940:Reishi / Q000737:chemistry; D013302:Streptomyces / Q000737:chemistry
chemical_list: D015700:HIV Envelope Protein gp41; D011134:Polysaccharides