[Preparative separation of gentiopicrin from Gentiana scabra bunge by high speed counter current chromatography].
OBJECTIVE
High speed counter current chromatography was successfully applied to the preparative separation of the component of Gentiana scabra Bunge.


METHODS
Gentiopicrin was obtained by preparative HSCCC with two phase solvent system com-posed of chloroform-methanol-tert-butylmethyl-water (5:4:2:4). The mobile phase is the lower phase and operated at a flow-rate of 2.0 ml/min, while the apparatus rotated at 820 r/min.


RESULTS
High performance liquid chromatogra phic analysis of gentiopicrin was over 96.68%.


CONCLUSIONS
HSCCC is a useful method for the separation of natural products.
Wang
Yan-yan
YY
Institute of Special Wild Economic Animal and Plant Science, C. A. A. S. JiLin 132109, China.
Wang
Ying-ping
YP
Liu
Ji-yong
JY
Yan
Shi
S
Sun
Cheng-he
CH
Xu
Shi-quan
SQ
D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D030012:Gentiana / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D000432:Methanol; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry; D012997:Solvents
D005960:Glucosides; D012997:Solvents; D000432:Methanol