Effect of thalidomide on endostatin levels in retinal ischemia/reperfusion injury.
OBJECTIVE
To evaluate the effects of thalidomide treatment on endostatin production in ischemia/reperfusion-injured guinea pigs retinas.


METHODS
Control, ischemia, thalidomide/ischemia groups including 7 animals in each were formed from pigmented male guinea pigs. Retinal ischemia was induced by cannulating anterior chambers and lifting the bottle to a height of 205 cm for 90 min in the ischemia and thalidomide/ischemia groups. The thalidomide/ischemia group received thalidomide (300 mg/kg/day) via nasogastric tube 24 h before ischemia and during 7 days of reperfusion. Guinea pigs were sacrified for biochemical analysis of retinal endostatin level by ELISA.


RESULTS
The mean retinal endostatin levels of the control, ischemia and thalidomide/ischemia groups were 11.79 +/- 7.36, 14.70 +/- 4.88, 30.94 +/- 15.59 ng/ml, respectively. The mean retinal endostatin level of the thalidomide/ischemia group was significantly higher than that of the control group (p = 0.011). The mean retinal endostatin level of the thalidomide/ischemia group was higher than that of the ischemia group, but there was no statistically significant difference (p = 0.073). No statistically significant difference in the mean retinal endostatin level was found between the control and ischemia groups (p = 0.165).


CONCLUSIONS
Thalidomide treatment increased retinal endostatin level in ischemia/reperfusion-injured guinea pig retinas.
Aydoğan
Semih
S
Department of Ophthalmology, Firat University School of Medicine, Elaziğ, Turkey.
Türkçüoğlu
Peykan
P
Celiker
Ulkü
U
Ilhan
Nevin
N
D000818:Animals; D043169:Endostatins / Q000378:metabolism*; D006168:Guinea Pigs; D007441:Intubation, Gastrointestinal; D008297:Male; D015427:Reperfusion Injury / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*; D012171:Retinal Vessels; D013792:Thalidomide / Q000008:administration & dosage / Q000494:pharmacology*
D043169:Endostatins; D013792:Thalidomide