title: Selective modulation of scleral proteoglycan mRNA levels during minus lens compensation and recovery.
abstract: OBJECTIVE
  To better characterize the role of proteoglycans in scleral tissue remodeling during the development of minus lens induced myopia and during recovery in tree shrews.
  
  
  METHODS
  Competitive reverse-transcription polymerase chain reaction (RT-PCR) was used to quantify the scleral mRNA levels for aggrecan, decorin, biglycan, and lumican in a group of tree shrews following four days of monocular -5 D lens treatment (n=5) and in a group after two days of recovery after 11 days of -5 D lens wear (n=5). Values were compared with age-matched normal animals (n=5). Aggrecan was localized within the sclera using immunohistochemistry.
  
  
  RESULTS
  Four days of -5 D lens wear produced axial (vitreous chamber) elongation and a myopic shift in the treated eyes. Two days of recovery produced significant refractive recovery. Aggrecan mRNA levels showed differential, bidirectional regulation. Levels in the treated eye sclera relative to the control eye were 30.8%+/-2.4% lower after four days of -5 D lens treatment and 51.4%+/-2.4% higher after two days of recovery. Decorin, biglycan, and lumican mRNA levels showed little differential regulation. However, biglycan and lumican along with aggrecan showed binocular regulation (treated and control eye mRNA levels significantly lower than normal eye mRNA levels after 4 days of -5D lens treatment). Immunohistochemical results showed that aggrecan is present in tree shrew sclera and that it is located primarily between the collagen lamella and near the fibroblasts.
  
  
  CONCLUSIONS
  These data suggest that the expression of aggrecan is strongly differentially modulated in the sclera during experimentally induced myopia and recovery. The modulation of aggrecan in concert with previously described changes in type I collagen and hyaluronan may play a key functional role in modulating the ability of the lamellae to slip across one another. This may be manifested in the scleral creep rate, which in turn modulates axial elongation rate and refractive state.
authors.affiliation: Department of Vision Sciences, School of Optometry, The University of Alabama at Birmingham, Birmingham, AL, USA. siegwart@uab.edu
authors.forename: John Thomas
authors.initials: JT
authors.lastname: Siegwart
authors.forename: Christianne E
authors.initials: CE
authors.lastname: Strang
mesh_terms: D000222:Adaptation, Physiological; D053674:Aggrecans / Q000235:genetics / Q000378:metabolism; D000818:Animals; D058578:Biglycan; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics; D058575:Decorin; D016326:Extracellular Matrix Proteins / Q000235:genetics; D007150:Immunohistochemistry; D007632:Keratan Sulfate / Q000235:genetics; D007909:Lenses; D000071234:Lumican; D009216:Myopia / Q000209:etiology* / Q000378:metabolism* / Q000503:physiopathology; D011509:Proteoglycans / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D020127:Recovery of Function; D012029:Refraction, Ocular; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012590:Sclera / Q000378:metabolism*; D013997:Time Factors; D014418:Tupaiidae
chemical_list: D053674:Aggrecans; D058578:Biglycan; D011508:Chondroitin Sulfate Proteoglycans; D058575:Decorin; D016326:Extracellular Matrix Proteins; D000071234:Lumican; D011509:Proteoglycans; D012333:RNA, Messenger; D007632:Keratan Sulfate