title: High-yield synthesis of wax esters catalysed by modified Candida rugosa lipase.
abstract: Candida rugosa lipase (CRL) was applied in a non-solvent esterification reaction to yield twelve wax esters. All products were obtained in nearly 100% yield for 10 h at 50 degrees C when immobilized PEG(2000)-activated C. rugosa lipase was added to the reaction mixture. The surfactant had also a beneficial effect on the stability of the biocatalytic preparation with 83% of its activity conserved after the seventh run of repeated batch reactions.
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria. maiag@orgchm.bas.bg
authors.forename: M H
authors.initials: MH
authors.lastname: Guncheva
authors.forename: D
authors.initials: D
authors.lastname: Zhiryakova
mesh_terms: D000438:Alcohols / Q000378:metabolism; D002175:Candida / Q000201:enzymology; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004951:Esterification; D004952:Esters / Q000302:isolation & purification / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods*; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D014885:Waxes / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000438:Alcohols; D004800:Enzymes, Immobilized; D004952:Esters; D005227:Fatty Acids; D014885:Waxes; D008049:Lipase