title: The effects inhibiting the proliferation of cancer cells by far-infrared radiation (FIR) are controlled by the basal expression level of heat shock protein (HSP) 70A.
abstract: We developed a tissue culture incubator that can continuously irradiate cells with far-infrared radiation (FIR) of wavelengths between 4 and 20 microm with a peak of 7-12 microm, and found that FIR caused different inhibiting effects to five human cancer cell lines, namely A431 (vulva), HSC3 (tongue), Sa3 (gingiva), A549 (lung), and MCF7 (breast). Then, in order to make clear the control system for the effect of FIR, the gene expression concerned to the inhibition effect by FIR were analyzed. In consequence, basal expression level of HSP70A mRNA was higher in A431 and MCF7 cells than in the FIR-sensitive HSC3, Sa3, and A549 cells. Also, the over expression of HSP70 inhibited FIR-induced growth arrest in HSC3 cells, and an HSP70 siRNA inhibited the proliferation of A431 cells by irradiation with FIR. These results indicate that the effect of a body temperature range of FIR suppressing the proliferation of some cancer cells is controlled by the basal expression level of heat shock protein (HSP) 70A. This finding suggested that FIR should be very effective medical treatment for some cancer cells which have a low level of HSP70. Still more, if the level of HSP70 in any cancer of a patient was measured, the effect of medical treatment by FIR can be foreseen for the cancer.
authors.affiliation: Department of Oral and Maxillofacial Anatomy, Medical Science for Oral and Maxillofacial Regeneration, Graduate School of Health Biosciences, University of Tokushima, 3-18-15 Kuramoto, Tokushima 770-8504, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Ishibashi
authors.forename: Kikuji
authors.initials: K
authors.lastname: Yamashita
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Hosokawa
authors.forename: Kaori
authors.initials: K
authors.lastname: Sumida
authors.forename: Masaru
authors.initials: M
authors.lastname: Nagayama
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Kitamura
mesh_terms: D001973:Bromodeoxyuridine / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018840:HSP70 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D006801:Humans; D007259:Infrared Rays / Q000627:therapeutic use*; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis; D034741:RNA, Small Interfering / Q000494:pharmacology
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D001973:Bromodeoxyuridine