title: Alternative bioreactor strategy for probing infection and production.
abstract: Probing the baculovirus infection process is essential in optimizing the recombinant protein production. Typically, researchers monitor the infection process in the stirred tank reactor, which, however, contains a population of cells infected at different times after virus inoculation. This chapter describes a two-stage reactor system consisting of an upstream continuous stirred tank reactor and a downstream tubular reactor with segmented plug flow for probing baculovirus infection and production.
authors.affiliation: Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
authors.forename: Yu-Chen
authors.initials: YC
authors.lastname: Hu
authors.forename: William E
authors.initials: WE
authors.lastname: Bentley
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D019149:Bioreactors; D002460:Cell Line; D018411:Spodoptera / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D001616:beta-Galactosidase