Galectin-10 mRNA is overexpressed in peripheral blood of aspirin-induced asthma.
BACKGROUND
In sensitive patients, aspirin is associated with nasal and bronchial inflammation, eliciting local symptoms. Although the disease is clinically well characterized, its physiopathology is incompletely understood and noninvasive procedures, allowing an effective distinction between aspirin-induced asthma (AIA) and aspirin-tolerant asthma (ATA) are missing.


OBJECTIVE
The aims of the study were to compare AIA and ATA cohorts for clinical characteristics and to screen peripheral blood for differential mRNA expression.


METHODS
Patients experiencing symptoms following aspirin ingestion were considered as aspirin sensitive. Peripheral blood was collected to quantify mRNA expression, using microarray technology and quantitative RT-PCR.


RESULTS
Data indicated that AIA and ATA share large number of similarities for clinical phenotype. Screening of mRNA expression using microarray showed an overexpression of galectin-10 mRNA in AIA (AIA/ATA ratio = 1.9, P < 0.05). Results were confirmed using qRT-PCR. A positive correlation was established between microarray and qRT-PCR results for galectin-10 mRNA expression (r = 0.92, P < 0.0001). Finally, qRT-PCR results were validated on a subset of asthmatics and controls, showing an increased expression of galectin-10 mRNA in AIA vs ATA (P < 0.001) and vs controls (P < 0.01).


CONCLUSIONS
Our results demonstrate that AIA and ATA remain difficult to distinguish using clinical criteria. Employing two molecular biological methods, we demonstrate that galectin-10 mRNA is overexpressed in AIA, suggesting a novel candidate gene and a potentially innovative pathway for mucosal inflammation in aspirin intolerance.
Devouassoux
G
G
Respiratory Disease Unit, CHLS, HCL, France.
Pachot
A
A
Laforest
L
L
Diasparra
J
J
Freymond
N
N
Van Ganse
E
E
Mougin
B
B
Pacheco
Y
Y
D000328:Adult; D001241:Aspirin / Q000009:adverse effects*; D001249:Asthma / Q000097:blood* / Q000139:chemically induced; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004342:Drug Hypersensitivity / Q000097:blood* / Q000209:etiology; D005260:Female; D037161:Galectins / Q000097:blood* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D011336:Probability; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric
D015415:Biomarkers; D037161:Galectins; D012333:RNA, Messenger; D001241:Aspirin