title: Membranes from acrylonitrile-based polymers for selective cultivation of human keratinocytes.
abstract: A cell carrier made from synthetic material supporting selective growth of keratinocytes is a promising approach to avoid the phenomenon of fibroblast overgrowth during in vitro culture of skin substitutes. Therefore, we investigated polymer membranes made of polyacrylonitrile and copolymers of acrylonitrile and N-vinylpyrrolidone (NVP) for their ability to support selectively the growth of keratinocytes. It was found that a copolymer with an NVP-content of 30% (NVP30) supports growth of human keratinocyte cell line (HaCaT) cells and inhibits fibroblast growth under serum-containing conditions. Cell proliferation of HaCaT cells was measured over 14 days. If both cell types were cultured under serum-free conditions for initial adhesion over 6 h on these NVP30 polymers, they adhered to the same extent. Long-term experiments over 7 days were performed as a coculture of both cell types showing that HaCaT cells had a growth advantage that seems to be related to the paracrine activity of contaminating fibroblasts. As a result, confluent layers of HaCaT cells were obtained with small numbers of remaining fibroblasts. The new poly [acrylonitrile-co(NVP) membranes seem to be a promising culture system for the production of epidermal transplants.
authors.affiliation: Institute of Polymer Research, GKSS Research Center Geesthacht, Teltow, Germany.
authors.forename: Gregor
authors.initials: G
authors.lastname: Boese
authors.forename: Christiane
authors.initials: C
authors.lastname: Trimpert
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Albrecht
authors.forename: Günter
authors.initials: G
authors.lastname: Malsch
authors.forename: Thomas
authors.initials: T
authors.lastname: Groth
authors.forename: Andreas
authors.initials: A
authors.lastname: Lendlein
mesh_terms: D000181:Acrylonitrile / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000502:physiology*; D008422:Materials Testing; D008567:Membranes, Artificial; D011760:Pyrrolidinones / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011760:Pyrrolidinones; C042670:N-vinyl-2-pyrrolidinone; D000181:Acrylonitrile