Measurement of glycosaminoglycan release from cartilage explants.
Quantitation of glycosaminoglycans (GAGs) in the form of aggrecan fragments released from cartilage in culture is a simple way to determine the efficacy of different cytokines alone or in combination in simulating cartilage catabolism. Two approaches for GAG assay are described, with special attention being paid to the advantages and limitations of each method.
Mort
John S
JS
Roughley
Peter J
PJ
D053674:Aggrecans / Q000378:metabolism; D000818:Animals; D002227:Carbazoles / Q000032:analysis; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism*; D002417:Cattle; D004396:Coloring Agents; D016207:Cytokines / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000032:analysis / Q000378:metabolism* / Q000592:standards; D008751:Methylene Blue / Q000031:analogs & derivatives; D012015:Reference Standards; D046509:Tissue Culture Techniques; D014574:Uronic Acids / Q000032:analysis
D053674:Aggrecans; D002227:Carbazoles; D004396:Coloring Agents; D016207:Cytokines; D006025:Glycosaminoglycans; D014574:Uronic Acids; C435946:dimethylmethylene blue; C041514:carbazole; D008751:Methylene Blue