Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C).
To investigate the specialization of the two Arabidopsis CDC27 subunits in the anaphase-promoting complex (APC/C), we analyzed novel alleles of HBT/CDC27B and CDC27A, and characterized the expression of complementing HOBBIT (HBT) protein fusions in plant meristems and during the cell cycle. In contrast to other APC/C mutants, which are gametophytic lethal, phenotypes of weak and null hbt alleles indicate a primary role in the control of post-embryonic cell division and cell elongation, whereas cdc27a nulls are phenotypically indistinguishable from the wild type. However, cdc27a hbt double-mutant gametes are non-viable, indicating a redundant requirement for both CDC27 subunits during gametogenesis. Yeast-two-hybrid and pulldown studies with APC/C components suggest that the two Arabidopsis CDC27 subunits participate in several complexes that are differentially required during plant development. Loss-of-function analysis, as well as cyclin B reporter protein accumulation, indicates a conserved role for the plant APC/C in controlling mitotic progression and cell differentiation during the entire life cycle.
Pérez-Pérez
José M
JM
Department of Molecular Genetics, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.
Serralbo
Olivier
O
Vanstraelen
Marleen
M
González
Cristina
C
Criqui
Marie-Claire
MC
Genschik
Pascal
P
Kondorosi
Eva
E
Scheres
Ben
B
D000483:Alleles; D064173:Anaphase-Promoting Complex-Cyclosome; D017360:Arabidopsis / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004258:DNA Polymerase III / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017343:Genes, Plant; D008938:Mitosis / Q000502:physiology; D021122:Protein Subunits; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D043743:Ubiquitin-Protein Ligase Complexes / Q000378:metabolism*
D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D021122:Protein Subunits; D029702:Schizosaccharomyces pombe Proteins; C513024:cdc27 protein, S pombe; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D004258:DNA Polymerase III