Synthesis of phosphorothioate oligonucleotide-peptide conjugates by solid phase fragment condensation.
Phosphorothioate oligonucleotide-peptide conjugates were synthesized by solid phase fragment condensation (SPFC). Arginine rich peptides could be successfully conjugated in 2.8-13.4% isolated yields. All the products were fully characterized by reversed phase HPLC and MALDI-TOF-MS to give satisfactory results.
Diala
Irmina
I
School of Humanity Oriented Science and Technology, Kinki University, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.
Osada
Akira
A
Maruoka
Shinji
S
Imanisi
Takashi
T
Murao
Satoshi
S
Ato
Taishi
T
Ohba
Hideki
H
Fujii
Masayuki
M
D000595:Amino Acid Sequence; D002412:Cations; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000494:pharmacology; D010446:Peptide Fragments / Q000032:analysis / Q000138:chemical synthesis*; D054735:Phosphorothioate Oligonucleotides / Q000032:analysis / Q000138:chemical synthesis* / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D019098:Telomerase / Q000037:antagonists & inhibitors
D002412:Cations; D016376:Oligonucleotides, Antisense; D010446:Peptide Fragments; D054735:Phosphorothioate Oligonucleotides; D019098:Telomerase