[Study on extraction and antigenicity of the outer-membrane-protein of Bordetella avium].
To study the immunogenicity of Bordetella avium OMP, ultrasonic dispersion, TritonX-100 technique were used to extract Bordetella avium OMP. Its content was determined by Bradford method and it was detected by SDS-PAGE. Then OMP immunizing antigen was prepared and 1-day chicken was vaccinated by hypodermic inoculation, with the immunizing does of 0.3mL (OMP90microg), 0.5mL (OMP150microg), 0.8mL (OMP240microg), respectively. Results showed that content of Bordetella avium OMP is 300 g/mL, the best immunizing does is 0.5mL each one and Chickens can be protected against Bordetella avium at fetal dose if antibody titer is over 1:2(8). We can see from the antibody level detected by indirect ELISA that antibody can last long enough to help chickens pass susceptible period, so OMP has good immunogenicity. Results of this study lay good foundation for the development of monoclonal antibody to OMP, rapid diagnosis kit and subunit vaccine.
Hu
Xiao-na
XN
College of Animal Science and Veterinary Medicine of Shandong Agricultural University, Tai'an 271018, China. xiaonahu2000@163.com
Zhu
Rui-liang
RL
Liu
Hong-zhen
HZ
Bi
Jian-min
JM
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification*; D001885:Bordetella Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D042482:Bordetella avium / Q000737:chemistry / Q000276:immunology*; D002645:Chickens; D007114:Immunization; D017830:Octoxynol / Q000737:chemistry; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D011817:Rabbits
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D017830:Octoxynol