title: [Determination of citrinin in red kojic by HPCE].
abstract: OBJECTIVE
  To establish an instant determination method of citrinin in red kojic by high performance capillary electrophorphores for the first time.
  
  
  METHODS
  Red kojic was extracted with the mixtrue of Toluene and ethyl acetate (70:30). Separation was carried out in an uncoated fused silica capillary (50 microm x 45.0 cm). Meanwhile, a running voltage 15 kV, 20 mmol L(-1) borax buffer with 10.0 mmol L(-1) sodium deoxycholate (pH 9.3) and a UV detector at 212 nm were adopted.
  
  
  RESULTS
  Regression equation of citrinin was Y=9434 + 16781X (r =0.990), The lower limit of quantification (S/N > or =3) was 0.5 mg mL(-1). The assay coefficients of variation ranged from 98.8% to 101.1%. The intra and inter recovery ranged from 0.83 to 1.54% and from 1.86 to 5.09%. Twenty samples were determined with the method.
  
  
  CONCLUSIONS
  The method is proved to be simple, rapid and accurate, and it can be used to determine citrinin in red kojic.
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burns, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. chenjpro@mail.tmmu.com.cn
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Zhang-wan
authors.initials: ZW
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Qi
authors.forename: Zhong-rong
authors.initials: ZR
authors.lastname: Liu
authors.forename: Bo-gang
authors.initials: BG
authors.lastname: Li
mesh_terms: D002953:Citrinin / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D032741:Monascus / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D002953:Citrinin