[Enzymatic extraction of baicalein and wogonin from radix scutellariae].
OBJECTIVE
The optimum enzymatic extraction of baicalein and wogonin from Radix scutellariae were determined.


METHODS
HPLC method was used to determine the content of baicalein and wogoinin from Radix Scutellariae, 5 factors were studied on enznmalic extraction of celluase.


RESULTS
The optimum enzymatic extraction of celluase were determined as follows enzyme 20U for Ig material, pH 4.8, buffer to raw material ratio20, temperature 50 degrees C, and reaction time 8 h.


CONCLUSIONS
Compared with the traditional water extraction, the extraction yield of baicalein and wogonin increase 5 and 4 times.
Wang
Hong-zhi
HZ
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Yu
Chun-hao
CH
Gao
Jun
J
Zhao
Guang-rong
GR
D002480:Cellulase / Q000378:metabolism; D044950:Flavanones / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010946:Plants, Medicinal / Q000737:chemistry*; D031364:Scutellaria baicalensis / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D044950:Flavanones; C006680:baicalein; D002480:Cellulase; C085514:wogonin