Regulation of nociceptin/orphaninFQ secretion by immune cells and functional modulation of interleukin-2.
Previous research has demonstrated that numerous populations of immune cell, including lymphocytes, synthesize nociceptin (N/OFQ) precursor mRNA although little is known regarding the immunological role of N/OFQ. In the present study we have demonstrated significant effects of mitogens, pro-inflammatory cytokines, cyclic AMP analogues, glucocorticoids and CRF on N/OFQ secretion by rat splenocytes in vitro. N/OFQ (10(-14) to 10(-10)M) was also shown to inhibit proliferation of Con A-activated splenocytes and production of IL-2 in vitro. In summary we have shown how a variety of stimuli relevant to inflammation can regulate endogenous N/OFQ secretion by splenocytes in vitro. We also suggest that N/OFQ may promote anti-inflammatory actions via suppression of IL-2 in vivo.
Miller
Thomas R
TR
Department of Anatomy, University of Bristol, Southwell Street, Bristol, UK.
Fulford
Allison J
AJ
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005938:Glucocorticoids / Q000494:pharmacology; D053583:Interleukin-1beta / Q000494:pharmacology; D007376:Interleukin-2 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D018847:Opioid Peptides / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011965:Receptors, Glucocorticoid / Q000037:antagonists & inhibitors; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D000097629:Nociceptin
D015124:8-Bromo Cyclic Adenosine Monophosphate; D003907:Dexamethasone; D005938:Glucocorticoids; D053583:Interleukin-1beta; D007376:Interleukin-2; D008070:Lipopolysaccharides; D018847:Opioid Peptides; D011965:Receptors, Glucocorticoid; D014409:Tumor Necrosis Factor-alpha; D000097629:Nociceptin