[Evaluation of the infectivity of dengue 1 strains in the HepG2 and Vero cell lines].
Viral infectivity of Hawaii, 3 Peri and Riberao Pretto dengue 1 strains was evaluated in Vero and HepG2 cell lines by indirect immunofluorescence techniques. Dengue virus cellular tropism in vitro is diverse. They may replicate themselves in a great variety of cellular cultures, whose sensibility to viral infection is variable. The greatest percentage of infected cells in the HepG2 cell line was obtained with the highest multiplicities of infection (0.04 for Hawaii and Riberao Pretto strains and 0.01 for 3 Perú). The highest percentage of infected HepG2 and Vero cells for the studied strains and the greatest titer in the viral overnadant was obtained on the 5th day. Vero cell line was more sensitive to viral infection, since for the same multiplicity values it was detected a higher number of fluorescent cells and a better viral titre in the line of this overnadant than in the HepG2. The best result was obtained with the Hawaii strain that allowed to confirm faster the infection of the studied cellular lines.
Aguilar Barroso
Alicia
A
Instituto Finlay, Ciudad de La Habana. aaguilar@finlay.edu.cu
Amin Blanco
Nevis
N
Morier Díaz
Luis
L
Pérez Hernández
Ela María
EM
D000818:Animals; D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor / Q000821:virology*; D002522:Chlorocebus aethiops; D003470:Culture Media; D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000302:isolation & purification / Q000472:pathogenicity*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008113:Liver Neoplasms; D019595:Severe Dengue / Q000821:virology; D013997:Time Factors; D014709:Vero Cells / Q000821:virology*
D003470:Culture Media