title: Global gene expression profiling of wild type and lysC knockout Escherichia coli W3110.
abstract: Aspartokinase III, encoded by lysC, is responsible for the first step of lysine biosynthesis in Escherichia coli. In this study, a lysC knockout E. coli W3110 strain was generated to study the differential gene expression profiles of wild type and lysC knockout strains. Several significant changes were observed, including biosynthesis of lysine, oxaloacetate, alpha-ketoglutarate and glutamate genes. Genes related to transporters and heat shock proteins were also affected by lysC knockout. The results indicated that the lysC knockout strain exhibited some phenomena similar to lysine starvation. The data generated by this study further clarify the systematic role of lysC in lysine biosynthesis.
authors.affiliation: Department of Life Science, Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan.
authors.forename: Daniel Yuen-Teh
authors.initials: DY
authors.lastname: Liu
authors.forename: Chia-Hsin
authors.initials: CH
authors.lastname: Liu
authors.forename: Ming-Tsong
authors.initials: MT
authors.lastname: Lai
authors.forename: Hua-Kuo
authors.initials: HK
authors.lastname: Lin
authors.forename: Tzong-Hsiung
authors.initials: TH
authors.lastname: Hseu
mesh_terms: D001222:Aspartate Kinase / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D029968:Escherichia coli Proteins; C116188:lysC aspartokinase; D001222:Aspartate Kinase