Hematopoietic effect of fractions from the enzyme-digested colla corii asini on mice with 5-fluorouracil induced anemia.
Effects of fractions A and B from enzyme-digested traditional Chinese medicine colla corii asini on mice with 5-fluorouracil-induced anemia were investigated. The purpose of this study was to further understand the hematopoietic activities and mechanisms of colla corii asini. The fractions A and B were administered to anemic mice for 12 days. After confirming the anti-anemic effect of fractions A and B, we examined the effects of fractions A and B on immature granulocyte and erythroid cell activity and plasma cytokine level. Fraction A administration at 2 g/kg and 1 g/kg and fraction B administration at 1.6 g/kg and 0.8 g/kg activated granulocyte and erythrocyte progenitor cells in bone marrow and erythrocyte progenitors in spleen, led to the recovery of white blood cell and red blood cell counts, and increased the percentage of peripheral reticulocytes in red cells. The GM-CSF and EPO production determined by examining GM-CSF mRNA and EPO mRNA in the kidney and liver of the anemic mice were also enhanced. This treatment significantly increased serum GM-CSF and EPO level and lowered serum transforming growth factor (TGF-beta) level. These results suggested that fractions A and B promoted hematopoiesis by activating immature granulocyte and erythroid cells, partly by stimulating GM-CSF and EPO secretion and suppressing TGF-beta release. Identification of a specific peptide or protein is still required for the development of a novel medicine for anemia caused by malignancy or chemotherapy.
Wu
Hongzhong
H
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Yang
Fan
F
Cui
Shuya
S
Qin
Yufeng
Y
Liu
Jianwen
J
Zhang
Yuanxing
Y
D000740:Anemia / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism; D017931:DNA Primers; D004798:Enzymes / Q000378:metabolism*; D004921:Erythropoietin / Q000235:genetics; D005260:Female; D005472:Fluorouracil / Q000009:adverse effects*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics; D008516:Medicine, Chinese Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000097:blood
D017931:DNA Primers; D004798:Enzymes; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D004921:Erythropoietin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D005472:Fluorouracil