Zebrafish cdc25a is expressed during early development and limiting for post-blastoderm cell cycle progression.
Cdc25 phosphatases are required for eukaryotic cell cycle progression. To investigate mechanisms governing spatiotemporal dynamics of cell cycle progression during vertebrate development, we isolated two cdc25 genes from the zebrafish, Danio rerio, cdc25a, and cdc25d. We propose that Zebrafish cdc25a is the zebrafish orthologue of the tetrapod Cdc25A genes, while cdc25d is of indeterminate origin. We show that both genes have proliferation promoting activity, but that only cdc25d can complement a Schizosaccharomyces pombe loss of function cdc25 mutation. We present expression data demonstrating that cdc25d expression is very limited during early development, while cdc25a is widely expressed and consistent with the mitotic activity in previously identified mitotic domains of the post-blastoderm zebrafish embryo. Finally, we show that cdc25a can accelerate the entry of post-blastoderm cells into mitosis, suggesting that levels of cdc25a are rate limiting for cell cycle progression during gastrulation.
Nogare
Damian E Dalle
DE
Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005, USA.
Arguello
Andrea
A
Sazer
Shelley
S
Lane
Mary Ellen
ME
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D054262:Gastrulation / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D010802:Phylogeny; D012568:Schizosaccharomyces / Q000166:cytology / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*; D020687:cdc25 Phosphatases / Q000235:genetics*
D017931:DNA Primers; D020687:cdc25 Phosphatases