[Genome sequencing and analysis of foot-and-mouth disease virus Asia1/YNBS/58 strain].
The full-length genomic sequence of foot-and-mouth disease virus (FMDV) Asia1/YNBS/58 strain was determined by RT-PCR and compared with other 17 reference strains. The results showed that the complete genome of Asia1/YNBS/58 was 8164nt long including a 1061-nt 5' untranslated region (UTR), a 6990-nt open reading frame (ORF), and a 113-nt 3'UTR. The homology analysis indicated that the UTR regions and non-structural proteins were more conserved than the structural proteins in FMDV. VP1 exhibited the lowest conservation and VP4 was exceptionally conserved. The VP1-, VP2-, and VP3-based phylogenetic trees were divided into distinct clusters according to different serotypes, while the other gene-based phylogenetic trees exhibited some degree of intercross among serotypes. This study is the first description of the full-length genomic sequence of FMDV Chinese serotype Asia1.
Chang
Hui-Yun
HY
Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. changhuiyun@126.com
Du
Jun-Zheng
JZ
Cong
Guo-Zheng
GZ
Shao
Jun-Jun
JJ
Lin
Tong
T
Xie
Qing-Ge
QG
D020413:3' Untranslated Regions / Q000737:chemistry; D036022:Capsid Proteins / Q000235:genetics; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics*; D016679:Genome, Viral; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D020413:3' Untranslated Regions; D036022:Capsid Proteins; C442501:VP1 protein, Foot-and-mouth disease virus; C442459:VP2 protein, Foot-and-mouth disease virus; C442460:VP3 protein, Foot-and-mouth disease virus