title: [Expressions of fibronectin and bone morphogenetic protein-2, 4 during development of mouse tooth germ].
abstract: OBJECTIVE
  The purpose of this study was to investigate the distribution and relationship of fibronectin (FN), bone morphogenetic protein (BMP) 2, 4 during development of mouse tooth germ.
  
  
  METHODS
  Rat embryonic first molars were collected from E14 (bud stage) and E18 (bell stage). The expressions of FN and BMP-2, 4 were analyzed with immunohistochemistry.
  
  
  RESULTS
  FN was located in the epithelium and dental mesenchyme on bud stage, but on bell stage, the FN was found at the region of differentiating odontoblasts and in the inner enamel epithelium, and also BMP-2, 4 were abundant mainly at the brisk region of differentiating odontoblasts.
  
  
  CONCLUSIONS
  The results demonstrate that BMP-2, 4 and FN play the important roles during bud stage and bell stage, and there may be synergy between BMP-2, 4 and FN in regulating differentiation and maturity of odontoblasts.
authors.affiliation: Department of Oral and Maxillofacial Surgery, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Tian
authors.forename: Xin
authors.initials: X
authors.lastname: Nie
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Yun-feng
authors.initials: YF
authors.lastname: Lin
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D055415:Bone Morphogenetic Protein 4 / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D051381:Rats; D017207:Rats, Sprague-Dawley; D014083:Tooth Germ / Q000196:embryology*
chemical_list: C525720:Bmp2 protein, rat; C525811:Bmp4 protein, rat; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D005353:Fibronectins