Development of high-throughput screening system by single-cell reaction using microchamber array chip.
To demonstrate the practical use of a novel high-throughput screening system by a single cell reaction for the rapid screening of combinatorially mutated beta-glucosidase 1 from Aspergillus oryzae, a yeast cell chip microchamber array in combination with yeast cell surface engineering was successfully developed.
Fukuda
Takeshi
T
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui 910-8507, Japan.
Kato-Murai
Michiko
M
Suye
Shin-Ichiro
S
Ueda
Mitsuyoshi
M
D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D001617:beta-Glucosidase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D001617:beta-Glucosidase