Automated segmentation and classification of high throughput yeast assay spots.
Several technologies for characterizing genes and proteins from humans and other organisms use yeast growth or color development as read outs. The yeast two-hybrid assay, for example, detects protein-protein interactions by measuring the growth of yeast on a specific solid medium, or the ability of the yeast to change color when grown on a medium containing a chromogenic substrate. Current systems for analyzing the results of these types of assays rely on subjective and inefficient scoring of growth or color by human experts. Here, an image analysis system is described for scoring yeast growth and color development in high throughput biological assays. The goal is to locate the spots and score them in color images of two types of plates named "X-Gal" and "growth assay" plates, with uniformly placed spots (cell areas) on each plate (both plates in one image). The scoring system relies on color for the X-Gal spots, and texture properties for the growth assay spots. A maximum likelihood projection-based segmentation is developed to automatically locate spots of yeast on each plate. Then color histogram and wavelet texture features are extracted for scoring using an optimal linear transformation. Finally, an artificial neural network is used to score the X-Gal and growth assay spots using the extracted features. The performance of the system is evaluated using spots of 60 images. After training the networks using training and validation sets, the system was assessed on the test set. The overall accuracies of 95.4% and 88.2% are achieved, respectively, for scoring the X-Gal and growth assay spots.
Jafari-Khouzani
Kourosh
K
Radiology Image Analysis Laboratory, Henry Ford Hospital, Detroit, MI 48202, USA. kjafari@rad.hfh.edu
Soltanian-Zadeh
Hamid
H
Fotouhi
Farshad
F
Parrish
Jodi R
JR
Finley
Russell L
RL
D000465:Algorithms; D001185:Artificial Intelligence; D003124:Colorimetry / Q000379:methods*; D005656:Fungal Proteins / Q000378:metabolism*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000502:physiology*
D005656:Fungal Proteins