title: Stage-specific modulation of neprilysin and aminopeptidase N in the limbic system during kindling progression.
abstract: Aminopeptidase N (APN) and neprilysin (NEP) inactivate neuropeptides released into the brain extracellular fluid. We previously showed that the expression of pyroglutamyl peptidase II (PPII), the TRH degrading ecto-enzyme, is regulated in rat brain by amygdaline kindling, a paradigm that activates neuronal pathways in the limbic system increasing the expression of several neuropeptides including TRH and opioids. To understand the specificity of this phenomenon, we studied APN and NEP expression in brains of partially or fully kindled rats (stage II and V), sacrificed 6 h after last stimulus, compared with sham-operated animals. In situ hybridization analysis of NEP mRNA levels showed decreased expression at stage II in CA1, CA2, olfactory tubercle and medial mammillary nucleus, and increased at stage V in CA1 and CA2 cells. These changes were specific for the ipsilateral side. APN mRNA levels, semi-quantified by RT-PCR, were decreased at stage II and increased at stage V, in frontal cortex-olfactory tubercle, and hippocampus. NEP and APN enzymatic activities, determined by fluorometric assays, followed similar variations to their respective mRNA levels. The coordinated changes (in some regions) of NEP and APN expression were opposite to those previously observed for PPII mRNA and activity levels in limbic regions. These results demonstrate that expression of ectopeptidases can be regulated when peptide neurons are activated and, that regulation is enzyme-, region-, and stage-specific.
authors.affiliation: Div. Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñíz, México D.F., México.
authors.forename: Patricia
authors.initials: P
authors.lastname: de Gortari
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Vargas
authors.forename: Adrián
authors.initials: A
authors.lastname: Martínez
authors.forename: Arlene I
authors.initials: AI
authors.lastname: García-Vázquez
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Uribe
authors.forename: Lucía
authors.initials: L
authors.lastname: Chávez-Gutiérrez
authors.forename: Víctor
authors.initials: V
authors.lastname: Magdaleno
authors.forename: Guy
authors.initials: G
authors.lastname: Boileau
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Charlí
authors.forename: Patricia
authors.initials: P
authors.lastname: Joseph-Bravo
mesh_terms: D000818:Animals; D018826:CD13 Antigens / Q000235:genetics / Q000378:metabolism*; D004558:Electric Stimulation; D017403:In Situ Hybridization; D007696:Kindling, Neurologic / Q000502:physiology*; D008032:Limbic System / Q000502:physiology*; D008297:Male; D015260:Neprilysin / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018826:CD13 Antigens; D015260:Neprilysin