Description of two new HLA-C alleles in a black South African population.
Two new HLA-C alleles, Cw*0333 and Cw*0217, were identified in a Black South African population. HLA-Cw*0333 differs from Cw*030201 by an A-->G substitution at nucleotide 323, yielding an unusual missense substitution of Cys for the conserved Tyr-84 at the antigen cleft terminus. Molecular modeling suggests that this alters the predicted interactions of this critical residue with the opposite alpha(2)-helix, the peptide COOH terminus and possibly KIR2DL2. The second allele, Cw*0217, differs from Cw*0205 by an A-->T substitution at nucleotide 368, resulting in a Tyr-->Phe substitution at residue 99 of the HLA-C beta-pleated sheet that likely influences peptide side-chain binding. Both Cw*0333 and Cw*0217 appear to have arisen by missense mutations, respectively, from the HLA-B*5801-Cw*0302 and B*080101-Cw*0205 haplotypes.
Minevich
G
G
Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
Kuhn
L
L
Polistena
C
C
Nuara
J
J
Winchester
R
R
D000328:Adult; D000483:Alleles; D044383:Black People / Q000235:genetics*; D002648:Child; D005260:Female; D005805:Genes, MHC Class I; D015235:HLA-B Antigens / Q000737:chemistry / Q000235:genetics; D015236:HLA-C Antigens / Q000737:chemistry / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D020125:Mutation, Missense; D013019:South Africa
D015235:HLA-B Antigens; D015236:HLA-C Antigens