title: [Expression in Escherichia coli of the gspd(l) gene of the type II secretion system in Leptospira borgpetersenii serovariety hardjo].
abstract: A fragment of 1.539 pb of the gene homologous to gspD of Hardjobovis was clonated in the pET28a vector and it was transformed into E. coli C43 and Rosetta strains. The sequence of GspD(L) showed 46 % of similitude with E. coli GspD secretin. The expression of recombinant GspD(L) was obtained in Rosetta strain.
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, UNAM, México.
authors.forename: Ernesto Armando Rodríguez
authors.initials: EA
authors.lastname: Reyes
authors.forename: Paul
authors.initials: P
authors.lastname: Cullen
authors.forename: Dieter
authors.initials: D
authors.lastname: Bulach
authors.forename: Ben
authors.initials: B
authors.lastname: Adler
authors.forename: David
authors.initials: D
authors.lastname: Haake
authors.forename: Alejandro
authors.initials: A
authors.lastname: de la Peña-Moctezuma
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D006801:Humans; D007919:Leptospira / Q000235:genetics* / Q000276:immunology; D007922:Leptospirosis / Q000235:genetics* / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D014169:Transformation, Bacterial
chemical_list: D029968:Escherichia coli Proteins