title: Equilibrium dynamics of spermine-induced plasmid DNA condensation revealed by fluorescence lifetime correlation spectroscopy.
abstract: The spermine-induced DNA condensation is a first-order phase transition. Here, we apply a novel technique fluorescence lifetime correlation spectroscopy to analyze this transition in a greater detail. We show that the method allows for the observation of the condensed and uncondensed molecules simultaneously based solely on different fluorescence lifetimes of the intercalating fluorophore PicoGreen in the folded und unfolded domains of DNA. The auto- and cross-correlation functions reveal that a small fraction of the DNA molecules is involved in the dynamic intramolecular equilibrium. Careful inspection of the cross-correlation curves suggests that folding occurs gradually within milliseconds.
authors.forename: Jana
authors.initials: J
authors.lastname: Humpolícková
authors.forename: Ales
authors.initials: A
authors.lastname: Benda
authors.forename: Jan
authors.initials: J
authors.lastname: Sýkora
authors.forename: Radek
authors.initials: R
authors.lastname: Machán
authors.forename: Teresa
authors.initials: T
authors.lastname: Kral
authors.forename: Barbara
authors.initials: B
authors.lastname: Gasinska
authors.forename: Joerg
authors.initials: J
authors.lastname: Enderlein
authors.forename: Martin
authors.initials: M
authors.lastname: Hof
mesh_terms: D003198:Computer Simulation; D008956:Models, Chemical; D008958:Models, Molecular; D009038:Motion; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000737:chemistry* / Q000648:ultrastructure*; D013096:Spermine / Q000737:chemistry*
chemical_list: D013096:Spermine