Suppression of alpha smooth muscle actin expression by IFN-gamma in established myofibroblast cell lines.
The purpose of this study is to investigate the effect of interferon-gamma (IFN-gamma) on myofibroblast generation using four myofibroblast cell lines that we established from Dupuytren's nodules. Cells were cultured in Earl's medium with 15% fetal bovine serum (FBS) and plated on a microplate at a density of 10(4) cells/well. These cells were treated with various concentrations of IFN-gamma (0-1000 U/mL) for 3 days. By cell-capture enzyme immunoassay (CC-EIA), immunofluorescence staining, and reverse transcriptase polymerase chain reaction (RT-PCR) analysis, we evaluated the effect of IFN-gamma on the expression of alpha smooth muscle actin (alphaSMA) in these cells. CC-EIA and RT-PCR analysis showed that IFN-gamma suppressed alphaSMA expression, and immunofluorescence microscopy demonstrated that IFN-gamma markedly decreased the number of alphaSMA-positive cells. These results showed that IFN-gamma strongly inhibits myofibroblast generation in established myofibroblast cell lines and suggest the possibility of using IFN-gamma therapy for contractile disease.
Tanaka
Kazunari
K
Department of Orthopedic Surgery, Osaka Medical College, Osaka, Japan. reh030@poh.osaka-med.ac.jp
Sano
Kouichi
K
Nakano
Takashi
T
Yuba
Kazuhide
K
Kinoshita
Mitsuo
M
D000199:Actins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D000199:Actins; D012333:RNA, Messenger; D007371:Interferon-gamma