Relationship between the quantity of Kaposi sarcoma-associated herpesvirus (KSHV) in peripheral blood and effusion fluid samples and KSHV-associated disease.
The Kaposi sarcoma-associated herpesvirus (KSHV)-DNA level was determined in samples from 71 patients with Kaposi sarcoma (KS), 28 patients with multicentric Castleman disease (MCD), and 8 patients with primary effusion lymphoma (PEL). KSHV-DNA levels were higher in patients with active KS or MCD than in those with KS or MCD in remission. Among patients with active disease, the highest KSHV-DNA levels were observed in effusion fluid samples from patients with PEL (7.2 log(10) copies/150,000 cells), followed by blood samples from patients with MCD and PEL (4.86 and 3.83 log(10) copies/150,000 cells, respectively), and the lowest levels were observed in blood samples from patients with KS (2.63 log(10) copies/150,000 cells). Determining the KSHV-DNA level may be useful in diagnosing KSHV-associated disease and for following up patients with KS when the development of MCD or PEL is suspected.
Marcelin
Anne-Geneviève
AG
Service de Virologie UPRES-EA 2387, Paris, France. anne-genevieve.marcelin@psl.aphp.fr
Motol
Jérome
J
Guihot
Amélie
A
Caumes
Eric
E
Viard
Jean-Paul
JP
Dussaix
Elisabeth
E
Cadranel
Jacques
J
Frances
Camille
C
Carcelain
Guislaine
G
Calvez
Vincent
V
Dupin
Nicolas
N
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001202:Ascitic Fluid / Q000821:virology; D018791:CD4 Lymphocyte Count; D005871:Castleman Disease / Q000150:complications / Q000821:virology*; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D015658:HIV Infections / Q000150:complications; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D008223:Lymphoma / Q000150:complications / Q000821:virology*; D008297:Male; D008875:Middle Aged; D010996:Pleural Effusion / Q000821:virology; D012189:Retrospective Studies; D012514:Sarcoma, Kaposi / Q000150:complications / Q000821:virology*; D019562:Viral Load
D004279:DNA, Viral