title: Characterization of hepatobiliary organic anion transporters in Long-Evans Cinnamon rats.
abstract: The liver plays important roles in the detoxification of xenobiotics. Hepatobiliary transporters contribute to hepatic uptake and efflux processes of xenobiotecs. Expressions of these transporters may be modulated under the condition of hepatic failure. Long-Evans Cinnamon (LEC) rats provide a pertinent model for basic and clinical studies on hepatitis. However, only a few reports describing the properties of hepatobiliary transporters in LEC rats have appeared in the literature. We investigated the expression levels of hepatobiliary transporters in LEC rats by real-time RT-PCR. We found that hepatic expressions of three sinusoidal organic anion transporters, Ntcp, Oatp1a1 and Oatp1a4, were decreased in LEC rats. However, no significant difference of the expressions of Mrp2 and Bsep, organic anion transporters located on canalicular membrane, were found between Wistar rats and LEC rats.
authors.affiliation: Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharmasciences, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Chiba
authors.forename: Shirou
authors.initials: S
authors.lastname: Itagaki
authors.forename: Masaki
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hirano
authors.forename: Ken
authors.initials: K
authors.lastname: Iseki
mesh_terms: D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters / Q000032:analysis; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D027361:Organic Anion Transporters / Q000032:analysis; D029363:Organic Anion Transporters, Sodium-Dependent / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D020332:Rats, Inbred LEC; D017208:Rats, Wistar; D013448:Sulfobromophthalein / Q000378:metabolism; D027981:Symporters / Q000032:analysis
chemical_list: D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters; C490548:Abcb11 protein, rat; C423013:Abcc2 protein, rat; D002352:Carrier Proteins; D027361:Organic Anion Transporters; D029363:Organic Anion Transporters, Sodium-Dependent; D012333:RNA, Messenger; D027981:Symporters; D013448:Sulfobromophthalein; C071908:sodium-bile acid cotransporter