Voltage clamp fluorimetry studies of mammalian voltage-gated K(+) channel gating.
VCF (voltage clamp fluorimetry) provides a powerful technique to observe real-time conformational changes that are associated with ion channel gating. The present review highlights the insights such experiments have provided in understanding Kv (voltage-gated potassium) channel gating, with particular emphasis on the study of mammalian Kv1 channels. Further applications of VCF that would contribute to our understanding of the modulation of Kv channels in health and disease are also discussed.
Claydon
T W
TW
Department of Anesthesiology, Pharmacology and Therapeutics, University of British Columbia, Vancouver, BC, Canada, V6T 1Z3. tclaydon@interchange.ubc.ca
Fedida
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D000818:Animals; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D015640:Ion Channel Gating; D015221:Potassium Channels / Q000502:physiology*
D015221:Potassium Channels