title: Superoxide anion production by neutrophils is associated with prevalent clinical manifestations in systemic lupus erythematosus.
abstract: To determine the relation between neutrophil function and the clinical characteristics of systemic lupus erythematosus (SLE), the superoxide anion (O2-) production by neutrophils, mediated by FcgammaR and FcgammaR/CR cooperation, was studied in 64 SLE patients classified according to their prevalent clinical manifestations. Three clinically distinct patterns were designated: (1) manifestations associated with the occurrence of cytotoxic antibodies (SLE-I group); (2) manifestations associated with circulating immune complexes (IC; SLE-II group), and (3) manifestations associated with IC and cytotoxic antibodies (SLE-III group). O2- production was evaluated by a lucigenin-dependent chemiluminescent assay in neutrophils stimulated with IC-IgG opsonized or not with complement. No difference in O2- production was observed when neutrophil responses from healthy controls were compared to the unclassified patients. However, when the SLE patient groups were considered, the following differences were observed: (1) SLE-I neutrophils showed lower O2- production mediated by the IgG receptor (FcgammaR) with the cooperation of complement receptors (FcgammaR/CR) than observed in the SLE-II, SLE-III, and healthy groups; (2) neutrophils from the SLE-II group showed a decreased [Formula: see text] production mediated by FcgammaR/CR compared to the SLE-III group, (3) SLE-III neutrophils produced more O(2)(-) than neutrophils from the SLE-II and control groups, and (4) CR showed inefficiency in mediating the O2- production by neutrophils from the SLE-I group. Comparative experiments on the kinetics of chemiluminescence (CL; Tmax and CLmax) disclosed differences only for the SLE-I group. Taken together, these results suggest that differences in oxidative metabolism of neutrophils mediated by FcgammaR/CR may reflect an acquired characteristic of disease associated with distinct clinical manifestations.
authors.affiliation: Departamento de Bioquímica e Imunologia da Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brasil.
authors.forename: Celene M O S
authors.initials: CM
authors.lastname: Alves
authors.forename: Cleni M
authors.initials: CM
authors.lastname: Marzocchi-Machado
authors.forename: Paulo
authors.initials: P
authors.lastname: Louzada-Junior
authors.forename: Ana Elisa C S
authors.initials: AE
authors.lastname: Azzolini
authors.forename: Ana Cristina M
authors.initials: AC
authors.lastname: Polizello
authors.forename: Ivan F
authors.initials: IF
authors.lastname: de Carvalho
authors.forename: Yara M
authors.initials: YM
authors.lastname: Lucisano-Valim
mesh_terms: D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood; D005260:Female; D006801:Humans; D007700:Kinetics; D049449:Luminescence; D008180:Lupus Erythematosus, Systemic / Q000453:epidemiology / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D011951:Receptors, Complement / Q000378:metabolism; D017452:Receptors, IgG / Q000378:metabolism; D016036:Seroepidemiologic Studies; D013481:Superoxides / Q000378:metabolism*
chemical_list: D001323:Autoantibodies; D011951:Receptors, Complement; D017452:Receptors, IgG; D013481:Superoxides