[Inverse PCR amplification of the complete major capsid protein gene of lymphocystis disease virus isolated from Rachycentron canadum and the phylogenetic analysis of the virus].
The major capsid protein of lymphocystis disease virus isolated from Rachycentron canadum (LCDV-rc) was amplified and analysed. The 457bp DNA core fragment was amplified with the degenerate primers designed according to the conserved sequences of MCP gene of iridoviruses, then the flaking sequences adjacent to the core region were amplified by inverse PCR, and the complete sequence was obtained by combining all of them. The open reading frame of the gene is 1380bp in length, encoding a putative protein of 459 aa with molecular weight 51.12 kD and pI 6.87. Constructing the phylogenetic tree for comparing the MCP amino acid of iridoviruses, the results indicated that LCDV-rc is most homologous to the other Lymphocystis viruses and all of them constitute a branch. Accordingly LCDV-rc is identified as Lymphocystivirus.
Fu
Xiao-Zhe
XZ
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China.
Shi
Cun-Bin
CB
Li
Ning-Qiu
NQ
Pan
Hou-Jun
HJ
Chang
Ou-Qin
OQ
Wu
Shu-Qin
SQ
D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics*; D007497:Iridoviridae / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010473:Perciformes / Q000382:microbiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*
D036022:Capsid Proteins