Application of supramolecular nanostamping to the replication of DNA nanoarrays.
The rapid development of molecular biology is creating a pressing need for arrays of biomolecules that are able to detect smaller and smaller volumes of analytes. This goal can be achieved by shrinking the average size and spacing of the arrays' constituent features. While bioarrays with dot size and spacing on the nanometer scale have been successfully fabricated via scanning probe microscopy-based techniques, such fabrication methods are serial in nature and consequently slow and expensive. Additionally, the development of truly small arrays able to analyze scarce volumes of liquids is hindered by the present use of optical detection, which sets the minimum dot spacing on the order of roughly half the excitation wavelength. Here, we show that supramolecular nanostamping, a recently introduced truly parallel method for the stamping of DNA features, can efficiently reproduce DNA arrays with features as small as 14 +/- 2 nm spaced 77 +/- 10 nm. Moreover, we demonstrate that hybridization of these nanoarrays can be detected using atomic force microscopy in a simple and scaleable way that additionally does not require labeling of the DNA strands.
Akbulut
Ozge
O
Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Jung
Jin-Mi
JM
Bennett
Ryan D
RD
Hu
Ying
Y
Jung
Hee-Tae
HT
Cohen
Robert E
RE
Mayes
Anne M
AM
Stellacci
Francesco
F
D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D018625:Microscopy, Atomic Force; D016252:Microscopy, Scanning Tunneling / Q000379:methods; D008968:Molecular Conformation; D053758:Nanoparticles / Q000737:chemistry*; D049329:Nanostructures; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*
D006046:Gold; D004247:DNA