title: Post-duplication charge evolution of phosphoglucose isomerases in teleost fishes through weak selection on many amino acid sites.
abstract: BACKGROUND
  The partitioning of ancestral functions among duplicated genes by neutral evolution, or subfunctionalization, has been considered the primary process for the evolution of novel proteins (neofunctionalization). Nonetheless, how a subfunctionalized protein can evolve into a more adaptive protein is poorly understood, mainly due to the limitations of current analytical methods, which can detect only strong selection for amino acid substitutions involved in adaptive molecular evolution. In this study, we employed a comparative evolutionary approach to this question, focusing on differences in the structural properties of a protein, specifically the electric charge, encoded by fish-specific duplicated phosphoglucose isomerase (Pgi) genes.
  
  
  RESULTS
  Full-length cDNA cloning, RT-PCR based gene expression analyses, and comparative sequence analyses showed that after subfunctionalization with respect to the expression organ of duplicate Pgi genes, the net electric charge of the PGI-1 protein expressed mainly in internal tissues became more negative, and that of PGI-2 expressed mainly in muscular tissues became more positive. The difference in net protein charge was attributable not to specific amino acid sites but to the sum of various amino acid sites located on the surface of the PGI molecule.
  
  
  CONCLUSIONS
  This finding suggests that the surface charge evolution of PGI proteins was not driven by strong selection on individual amino acid sites leading to permanent fixation of a particular residue, but rather was driven by weak selection on a large number of amino acid sites and consequently by steady directional and/or purifying selection on the overall structural properties of the protein, which is derived from many modifiable sites. The mode of molecular evolution presented here may be relevant to various cases of adaptive modification in proteins, such as hydrophobic properties, molecular size, and electric charge.
authors.affiliation: Division of Molecular Marine Biology, Ocean Research Institute, The University of Tokyo, 1-15-1 Minamidai, Nakano-ku, Tokyo 164-8639, Japan. ysato@ori.u-tokyo.ac.jp
authors.forename: Yukuto
authors.initials: Y
authors.lastname: Sato
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Nishida
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D019143:Evolution, Molecular; D005399:Fishes / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D020440:Gene Duplication; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics*; D057927:Hydrophobic and Hydrophilic Interactions; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012641:Selection, Genetic; D016415:Sequence Alignment; D017385:Sequence Homology; D013045:Species Specificity; D055672:Static Electricity; D013329:Structure-Activity Relationship
chemical_list: D007527:Isoenzymes; D005956:Glucose-6-Phosphate Isomerase