title: Tryptophan supplementation and pH adjustment for optimizing the sporulation of Coniothyrium minitans.
abstract: To determine the effect of tryptophan and pH on sporulation of Coniothyrium minitans, the fungus was cultivated using a two-stage, agar plate method in which addition of tryptophan and pH were controlled at the sporulation stage. The spore yield was enhanced by 4 times with 0.1 g tryptophan/l addition after 72 h. The optimal pH values were 4 for mycelia growth and 5.8-6 for sporulation. Mycelia grown at pH 6 had a higher productivity of spore production than did those grown at pH 4.
authors.affiliation: College of Food Science and Engineering, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China. sjlshi2004@yahoo.com.cn
authors.forename: Junling
authors.initials: J
authors.lastname: Shi
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhang
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D001203:Ascomycota / Q000187:drug effects / Q000254:growth & development*; D006863:Hydrogen-Ion Concentration; D013172:Spores, Fungal / Q000187:drug effects / Q000254:growth & development; D014364:Tryptophan / Q000494:pharmacology*
chemical_list: D014364:Tryptophan