title: [Evaluation of an homologous bacterin against bovine leptospirosis].
abstract: 48 adult bovine females dividided into 6 groups were used aimed at characterizing the immune response induced in breastfeeeding cows by an homologous bacterin formulated with different adjuvants. They were intramuscularly administered 2 milliliters of a bacterin formulated with Leptospira interrogans serovars uam, wolffi, hardjo, bratislava, grippotyphosa and panama added with different adjuvants, such as aluminum hydroxide, Freud's complete adjuvant, Freud's incomplete adjuvant, liposoluble vitamins, bacterin plus disparasitization with levamisol. The control group was administred only with bacterin. Immunization took place in 2 occasions at a time interval of 28 days. Blood samples were taken every 7 days during the first month after vaccination, and every 28 days for the next 8 months. All the sera were analyzed by the microscopic agglutination test. The results were transformed into Log10 and they were analyzed by NLIN and GLM of SAS. The period of greater response was estimated by the prediction model (Wood). The bacterin did not produce alteration either in the physiological constants, or in milk production. The serovars of Leptospira interrogans that induced higher titers were uam, hardjo and wolffi. The statistical difference between treatments and between serovars was determined.
authors.affiliation: Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (INIFAP), México. leorozco@yahoo.com.mx
authors.forename: Laura Elena Orozco
authors.initials: LE
authors.lastname: Vega
authors.forename: Rafael López
authors.initials: RL
authors.lastname: Flores
authors.forename: Luis Pedro
authors.initials: LP
authors.lastname: Moles y Cervantes
authors.forename: Jorge Quiroz
authors.initials: JQ
authors.lastname: Valiente
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000917:Antibody Formation; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control*; D003627:Data Interpretation, Statistical; D005260:Female; D005500:Follow-Up Studies; D005620:Freund's Adjuvant / Q000008:administration & dosage; D007273:Injections, Intramuscular; D007921:Leptospira interrogans / Q000145:classification / Q000276:immunology*; D007922:Leptospirosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D013997:Time Factors
chemical_list: D000276:Adjuvants, Immunologic; D001428:Bacterial Vaccines; D005620:Freund's Adjuvant