Analysis of TIMP expression and activity.
This chapter provides practical information on the assay of tissue inhibitor of metalloproteinase (TIMP) activity and background information enabling meaningful interpretation of the data. Protocols are given for assessing the presence of TIMPs in biological samples by immunoblotting and by virtue of their ability to inhibit matrix metalloproteinase (MMP) hydrolysis of protein substrates (reverse zymography) and synthetic fluorogenic substrates.
Troeberg
Linda
L
Nagase
Hideaki
H
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005456:Fluorescent Dyes; D005780:Gelatin; D006801:Humans; D007700:Kinetics; D061965:Matrix Metalloproteinase Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism
D005456:Fluorescent Dyes; D061965:Matrix Metalloproteinase Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases; D005780:Gelatin