Immobilized organelles and whole cells into protein foam structures: scanning and transmission electron microscope observations.
Protein foam structures bearing cells and organelles were produced by using a co-crosslinking method with serum albumin and glutaraldehyde at sub-zero temperature. Morphological observations obtained with scanning and transmission electron microscopy indicate macroporous and homogeneous structures. The glutaraldehyde concentration was varied to reveal its effect on immobilized red cells. It was observed that the structural appearance of preatreated cells or subcellular fractions (thylakoids from lettuce ; spheroplasts and chromatophores from R. capsulata) is preserved during the immobilization process. The morphological features of foam particles are always related to the observed kinetic activities of the specimens.
Barbotin
J N
JN
Laboratoire de Technologie Enzymatique, Université de Compiègne, (ERA No 338 du C.N.R.S.), BP 233, 60206 Compiègne, France.
Thomasset
B
B
D001773:Blood Cells / Q000378:metabolism; D002736:Chloroplasts / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D018545:Lactuca / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D011506:Proteins / Q000737:chemistry*; D013104:Spheroplasts / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D013696:Temperature; D020524:Thylakoids / Q000737:chemistry / Q000378:metabolism
D003432:Cross-Linking Reagents; D011506:Proteins