Endometriosis as a neurovascular condition: estrous variations in innervation, vascularization, and growth factor content of ectopic endometrial cysts in the rat.
Endometriosis is a poorly understood, estradiol-dependent condition associated with severe pelvic pains and defined by vascularized endometrial growths outside the uterus. Endometriosis is produced in cycling rats by autotransplanting pieces of uterus onto abdominal arteries where they develop into cysts. The surgery induces vaginal and abdominal muscle hyperalgesia, whose severity is greatest in proestrus and nearly absent in estrus. The cysts contain growth factors and cytokines and develop their own sympathetic and sensory C- and Adelta-fiber innervation. Here, we used quantitative immunostaining and protein array analyses to test the hypothesis that the innervation and growth factor/cytokine content of the cysts, but not uterine horn, contribute to proestrous-to-estrous changes in hyperalgesic severity. If so, these characteristics in the cysts, but not the uterine horn, should change with estrous stage. In cysts, the density of sympathetic (but not sensory) neurites and amounts of NGF and VEGF proteins (but not cytokines IL-1, IL-6, IL-10, or TNF-alpha) were greater in proestrus than estrus. These changes were accompanied by vascular changes. Both sympathetic and sensory fibers in both stages colabeled with TrkA, indicating that changes in NGF could act on both afferent and efferent fibers. In contrast with the cysts, no changes occurred in the uterine horn between proestrus and estrus. Together, these results suggest that coordinated proestrous-to-estrous changes in innervation and vascularization of the cysts contribute to similar changes in hyperalgesic severity. The findings also encourage consideration of endometriosis as a neurovascular condition.
Zhang
Guohua
G
Program in Neuroscience, Florida State University, Tallahassee, FL 32306-1270, USA.
Dmitrieva
Natalia
N
Liu
Yan
Y
McGinty
Kristina A
KA
Berkley
Karen J
KJ
D000320:Adrenergic Fibers / Q000473:pathology; D000818:Animals; D003560:Cysts / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004715:Endometriosis / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000098:blood supply / Q000294:innervation* / Q000378:metabolism; D030762:Estrous Cycle / Q000502:physiology*; D005260:Female; D006930:Hyperalgesia / Q000209:etiology / Q000378:metabolism; D020932:Nerve Growth Factor / Q000378:metabolism*; D011359:Proestrus / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism; D020917:Receptor, trkA / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D016207:Cytokines; D020800:Receptor, Nerve Growth Factor; D042461:Vascular Endothelial Growth Factor A; D020932:Nerve Growth Factor; D020917:Receptor, trkA