title: Co-regulation by Notch and Fos is required for cell fate specification of intermediate precursors during C. elegans uterine development.
abstract: The Notch pathway is the key signal for many cell fate decisions in the nematode Caenorhabditis elegans including the uterine pi cell fate, crucial for a proper uterine-vulval connection and egg laying. Expression of the egl-13 SOX domain transcription factor is specifically upregulated upon induction of the pi lineage and not in response to other LIN-12/Notch-mediated decisions. We determined that dual regulation by LIN-12 and FOS-1 is required for egl-13 expression at specification and for complete rescue of egl-13 mutants. We found that fos-1 mutants exhibit uterine defects and fail to express pi markers. We show that FOS-1 is expressed at pi cell specification and can bind in vitro to egl-13 upstream regulatory sequence (URS) as a heterodimer with C. elegans Jun.
authors.affiliation: Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Kavita S
authors.initials: KS
authors.lastname: Oommen
authors.forename: Anna P
authors.initials: AP
authors.lastname: Newman
mesh_terms: D000818:Animals; D001665:Binding Sites; D019521:Body Patterning / Q000235:genetics*; D017173:Caenorhabditis elegans / Q000196:embryology* / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism; D012734:Disorders of Sex Development / Q000196:embryology / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005260:Female; D005786:Gene Expression Regulation; D008565:Membrane Proteins / Q000502:physiology*; D008954:Models, Biological; D032390:Myoblasts, Smooth Muscle / Q000378:metabolism / Q000502:physiology*; D016759:Oncogene Protein p65(gag-jun) / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism / Q000502:physiology*; D051881:Receptor, Notch1 / Q000502:physiology*; D051880:Receptors, Notch; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014599:Uterus / Q000196:embryology* / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; D004268:DNA-Binding Proteins; C491546:EGL-13 protein, C elegans; C520083:FOS-1 protein, C elegans; C099355:LAG-1 protein, C elegans; C082460:Lin-12 protein, C elegans; D008565:Membrane Proteins; D016759:Oncogene Protein p65(gag-jun); D016760:Proto-Oncogene Proteins c-fos; D051881:Receptor, Notch1; D051880:Receptors, Notch; D014157:Transcription Factors