Comparative proteome analysis of neural retinas from type 2 diabetic rats by two-dimensional electrophoresis.
OBJECTIVE
The purpose of this study was to isolate, identify, and analyze diabetes-related protein changes that occur in neural retinas in vivo.


METHODS
Total proteins were extracted from neural retinas of normal and 8-weeks diabetic Sprague-Dawley (SD) rats and separated by two-dimensional gel electrophoresis (2-DE). Some protein spots exhibiting statistically significant variations (p < 0.05) were selected randomly and identified by mass spectrometry (MS or MS/MS). The protein alphaA-crystallin was chosen as a target for specific immunodetection using Western blot to corroborate the variation found by 2-DE.


RESULTS
Twenty protein spots identified included alphaA-crystallin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), glutamine (Gln) synthetase, and so forth. Western blotting analyses confirmed that alphaA-crystallin protein expression was upregulated in diabetic retina.


CONCLUSIONS
In this study, we isolate, identify, and analyze diabetes-related protein changes that occur in neural retinas in vivo. Further investigation of candidate proteins may identify novel pharmacological targets for diabetic retinopathy.
Wang
Ya-Dong
YD
Department of Ophthalmology, First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
Wu
Jin-Dao
JD
Jiang
Zhong-Li
ZL
Wang
Yu-bang
YB
Wang
Xue-Hao
XH
Liu
Chao
C
Tong
Ming-Qing
MQ
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000503:physiopathology; D004041:Dietary Fats / Q000008:administration & dosage; D015180:Electrophoresis, Gel, Two-Dimensional; D005136:Eye Proteins / Q000378:metabolism; D007328:Insulin / Q000097:blood; D008297:Male; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood; D038202:alpha-Crystallin A Chain / Q000378:metabolism
D001786:Blood Glucose; D004041:Dietary Fats; D005136:Eye Proteins; D007328:Insulin; D014280:Triglycerides; D038202:alpha-Crystallin A Chain; D002784:Cholesterol