title: Improvement of RT-PCR detection of chronic bee paralysis virus (CBPV) required by the description of genomic variability in French CBPV isolates.
abstract: A new RT-PCR test has been developed to diagnose Chronic bee paralysis virus (CBPV) that is able to detect genetically variable viral isolates. In fact, up to 8.7% divergence between partial nucleotide sequences from viral isolates from French honey bees was highlighted in a preliminary variability study. The previously-described RT-PCR was unable to detect all these viral isolates and RT-PCR diagnosis needed improvement. The new RT-PCR test can detect up to 40% more CBPV isolates.
authors.affiliation: Agence Française de Sécurité Sanitaire des Aliments (AFSSA) Sophia-Antipolis, Unité Pathologie de l'Abeille, Les Templiers, France. p.blanchard@afssa.fr
authors.forename: Philippe
authors.initials: P
authors.lastname: Blanchard
authors.forename: Violaine
authors.initials: V
authors.lastname: Olivier
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Iscache
authors.forename: Olivier
authors.initials: O
authors.lastname: Celle
authors.forename: Frank
authors.initials: F
authors.lastname: Schurr
authors.forename: Perrine
authors.initials: P
authors.lastname: Lallemand
authors.forename: Magali
authors.initials: M
authors.lastname: Ribière
mesh_terms: D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics*; D005602:France; D014644:Genetic Variation; D007304:Insect Viruses / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004279:DNA, Viral