title: Endosomes and lysosomes play distinct roles in sulfatide-induced neuroblastoma apoptosis: potential mechanisms contributing to abnormal sulfatide metabolism in related neuronal diseases.
abstract: Alterations in sulfatide metabolism, trafficking and homoeostasis are present at the earliest clinically recognizable stages of Alzheimer's disease and are associated with metachromatic leukodystrophy. However, the role of sulfatide in these disease states remains unknown. In the present study, we investigated the sequelae of NB (neuroblastoma) cells upon sulfatide supplementation and the biochemical mechanisms contributing to the sulfatide-induced changes. By using shotgun lipidomics, we showed dramatic accumulations of sulfatide, ceramide and sphingosine in NB cells in a time- and dose-dependent manner. Further studies utilizing subcellular fractionation and shotgun lipidomics analyses demonstrated that most of the increased ceramide content was generated in the endosomal compartment, whereas sulfatides predominantly accumulated in lysosomes. In addition, we determined that the sulfatide-mediated increase in endosomal ceramide content mainly resulted from beta-galactosidase activity, which directly hydrolyses sulfatide to ceramide without a prior desulfation step. Substantial cell apoptosis occurred in parallel with the accumulation of sulfatides and ceramides, as revealed by mitochondrial membrane depolarization, by phosphatidylserine translocation and by the TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling) assay. These findings were also demonstrated with primary neuron cultures. Collectively, our results demonstrate that abnormal sulfatide metabolism can induce cell apoptosis due to endosome-mediated ceramide generation and the accumulation of cytotoxic levels of sulfatides in lysosomes.
authors.affiliation: Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, Campus Box 8020, 660 South Euclid Avenue, St. Louis, MO 63110, USA.
authors.forename: Youchun
authors.initials: Y
authors.lastname: Zeng
authors.forename: Hua
authors.initials: H
authors.lastname: Cheng
authors.forename: Xuntian
authors.initials: X
authors.lastname: Jiang
authors.forename: Xianlin
authors.initials: X
authors.lastname: Han
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D011992:Endosomes / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry; D020287:In Situ Nick-End Labeling; D008247:Lysosomes / Q000502:physiology*; D013058:Mass Spectrometry; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology*; D011247:Pregnancy; D013050:Spectrometry, Fluorescence; D013433:Sulfoglycosphingolipids / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D013433:Sulfoglycosphingolipids