Cell trapping in activated micropores for functional analysis.
This paper presents a novel device which provides the opportunity to perform high-throughput biochemical assays on different individual cells. In particular, the proposed device is suited to screen the rare cells in biological samples for early stage cancer diagnosis and explore their biochemical functionality. In the process, single cells are precisely positioned and captured in activated micropores. To show the performance of the proposed device, cultured yeast cells and human epithelial circulating tumor cells are successfully captured.
Talasaz
AmirAli H
AH
Electr. Eng. Dept., Stanford Univ., CA 94305, USA. atalasaz@stanford.edu
Powell
Ashley A
AA
Stahl
Patrik
P
Ronaghi
Mostafa
M
Jeffrey
Stefanie S
SS
Mindrinos
Michael
M
Davis
Ronald W
RW
D001672:Biocompatible Materials / Q000737:chemistry*; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002468:Cell Physiological Phenomena; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D016062:Porosity; D013499:Surface Properties
D001672:Biocompatible Materials