title: A GPCR involved in post aggregation events in Dictyostelium discoideum.
abstract: Dictyostelium has 55 genes encoding seven-transmembrane G-protein-coupled receptors (GPCR) that belong to five of the six GPCR families. GrlA is one of the 17 family 3 GPCRs in Dictyostelium all of which resemble GABA(B) receptors from higher eukaryotes. GrlA is a 90-kDa protein present on the plasma membrane and on membranes of the ER. It has a large extracellular domain with homology to bacterial periplasmic proteins. The GrlA message is present throughout development and shows increased levels during the post aggregation stages. Inactivation of the grlA gene does not severely affect the growth phase, however, it leads to a delay in the development at the post aggregation stage. GrlA deficient strains show an altered DIF-1 response specific to the prestalk-specific ecmA and ecmB gene, reduced car2 and pkaC transcript levels and form a reduced number of spores. Germination of the spores was as in wild type. Transcriptional profiling supported the defect in the sporulation pathway as a large number of genes involved in the biogenesis and organization of the extracellular matrix and the sporulation process were significantly downregulated in the mutant.
authors.affiliation: Center for Biochemistry, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 52, 50931 Köln, Germany.
authors.forename: Yogikala
authors.initials: Y
authors.lastname: Prabhu
authors.forename: Subhanjan
authors.initials: S
authors.lastname: Mondal
authors.forename: Ludwig
authors.initials: L
authors.lastname: Eichinger
authors.forename: Angelika A
authors.initials: AA
authors.lastname: Noegel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D000242:Cyclic AMP / Q000494:pharmacology; D004252:DNA Mutational Analysis; D004023:Dictyostelium / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018390:Gene Targeting; D006588:Hexanones / Q000378:metabolism; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism; D008018:Life Cycle Stages / Q000187:drug effects; D008969:Molecular Sequence Data; D021381:Protein Transport / Q000187:drug effects; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D033761:Spores, Protozoan / Q000187:drug effects; D013347:Subcellular Fractions / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: C490570:Differentiation-inducing factor-1, Dictyostelium; D006588:Hexanones; D006843:Hydrocarbons, Chlorinated; D015800:Protozoan Proteins; D043562:Receptors, G-Protein-Coupled; D000242:Cyclic AMP