Comparison of changes in GAD65 and GAD67 immunoreactivity and levels in the gerbil main olfactory bulb induced by transient ischemia.
In the present study, we investigated changes in glutamate decarboxylase 65 (GAD65) and GAD67 immunoreactivity and protein levels in the main olfactory bulb (MOB) after 5 min of transient forebrain ischemia in gerbils. GAD65 immunoreactivity in the sham-operated group was shown in neurons and neuropil except for the somata of granule cells. GAD65 immunoreactivity was increased in neurons in the external plexiform layer 60 days after ischemia, and in mitral cells 30 and 60 days after ischemia. GAD67 immunoreactivity in the sham-operated group was shown in periglomerular cells, neuron in the external plexiform layer and granule cells with neuropil. GAD67 immunoreactivity in periglomerular cells was increased 10, 45 and 60 days after ischemia. GAD67 immunoreactivity in neurons in the external plexiform layer was increased 10 and 15 days after ischemia. Mitral cells showed strong GAD67 immunoreactivity 10 days after ischemia. However, GAD67 immunoreactivity in the granule cells was not changed with time after ischemia. In Western blot analysis for GAD65 and GAD67 protein levels in the ischemic gerbil MOB, GAD65 level was not changed after ischemia; GAD67 level was increased 10 days after ischemia. These results suggest that transient ischemia causes changes in GAD65 and GAD67 immunoreactivity in the gerbil MOB, and this change may induce a malfunction in olfaction after an ischemic insult.
Yoo
Ki-Yeon
KY
Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chuncheon 200-702, South Korea.
Hwang
In Koo
IK
Choi
Jung Hoon
JH
Seong
Je Kyung
JK
Yoon
Yeo Sung
YS
Lee
In Se
IS
Won
Moo-Ho
MH
D000818:Animals; D015153:Blotting, Western; D005849:Gerbillinae; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D007150:Immunohistochemistry; D007511:Ischemia / Q000201:enzymology*; D008297:Male; D009830:Olfactory Bulb / Q000098:blood supply / Q000201:enzymology*
D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1; C401141:glutamate decarboxylase 2