title: Drosophila Myc is required for normal DREF gene expression.
abstract: The Drosophila DNA replication-related element-binding factor (dDREF) is required for the expression of many proliferation-related genes carrying the DRE sequence, 5'-TATCGATA. Finding a canonical E-box, 5'-CACGTG, in the dDREF gene promoter prompted us to explore the possibility that the dDREF gene is a target of Drosophila Myc (dMyc). Luciferase transient expression assays combined with RNA interference in Drosophila S2 cells revealed that knockdown of dmyc reduced dDREF gene promoter activity by 35% to 82%, an effect at least partly mediated by the E-box in the promoter. dm(4)/Y hemizygous mutant larvae demonstrated no maternal dMyc and severe impairment of dDREF mRNA transcription. dMyc loss of function in dm(2)/dm(2) homozygous mutant follicle cell clones also resulted in loss of anti-dDREF immunostaining in nuclei. In contrast, co-expression of dMyc-dMax up-regulated dDREF promoter activity in S2 cells. Furthermore, dMyc over-expressing clones exhibited a high level of dDREF gene expression in wing and eye discs. These results taken together indicate that dMyc is indeed required for dDREF gene expression.
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Dang Thi Phuong
authors.initials: DT
authors.lastname: Thao
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Seto
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D002460:Cell Line; D002999:Clone Cells / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism*; D024721:E-Box Elements / Q000235:genetics*; D005123:Eye / Q000254:growth & development; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008297:Male; D011401:Promoter Regions, Genetic / Q000235:genetics*; D034622:RNA Interference / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics; D014921:Wings, Animal / Q000254:growth & development
chemical_list: D004268:DNA-Binding Proteins; C099494:Dref protein, Drosophila; D029721:Drosophila Proteins; C102284:Myc protein, Drosophila; D012333:RNA, Messenger; D014157:Transcription Factors