Confocal microscopy study of the distribution, content and activity of mitochondria during Paracentrotus lividus development.
In the present paper we applied confocal microscopy and fluorescence technologies for studying the distribution and the oxidative activity of sea urchin (Paracentrotus lividus) mitochondria during development, by in vivo incubating eggs and embryos with cell-permeant MitoTracker probes. We calculated, by a mathematical model, the intensity values, the variations of intensity, and the variation index of incorporated fluorochromes. Data demonstrate that mitochondrial mass does not change during development, whereas mitochondrial respiration increases. In addition, starting from 16 blastomeres stage, some regions of the embryo contain organelles more active in oxygen consumption.
Morici
Giovanni
G
Dipartimento di Biologia Cellulare e dello Sviluppo A. Monroy, Università degli Studi di Palermo, viale delle Scienze, ed. 16, 90128 Palermo, Italy.
Agnello
Maria
M
Spagnolo
Filippo
F
Roccheri
Maria Carmela
MC
Di Liegro
Carlo Maria
CM
Rinaldi
Anna Maria
AM
D000818:Animals; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000032:analysis / Q000378:metabolism; D047328:Paracentrotus / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure
D010100:Oxygen