Induction of murine AA amyloidosis by various homogeneous amyloid fibrils and amyloid-like synthetic peptides.
We investigated amyloid-enhancing factor (AEF) activity of amyloid fibrils extracted from amyloid-laden livers of mice, cow, cheetah, cat and swan. All amyloid fibrils were confirmed to be amyloid protein A (AA) by an immunohistochemical analysis. We found that these fibrils accelerated the deposition of amyloid in an experimental mouse model of AA amyloidosis. Furthermore, the degree of deposition was dependent on the concentration of fibrils. When we compared the minimal concentration of amyloid fibrils needed to induce deposition, we found that these fibrils showed different efficiencies. Murine amyloid fibril induced amyloid deposition more efficiently than cow, cat, cheetah or swan amyloid fibrils. These data suggest that amyloid deposition is preferentially induced by amyloid fibrils with the same primary sequence as the endogenous amyloid protein. We then analysed the AEF activity of synthetic peptides, synthesized corresponding to amino acids 1-15 of mouse SAA (mSAA), 2-15 of cow SAA (bSAA), 1-15 of cat SAA (cSAA), which was the same as cheetah, and the common amino acids 33-45 of these four SAA (aSAA). We found that mSAA, bSAA and cSAA formed amyloid-like fibrils in morphology and showed similar AEF properties to those of native amyloid fibrils. Although aSAA also formed highly ordered amyloid-like fibrils, it showed weaker AEF activity than the other synthetic fibrils. Our results indicate that amyloidosis is transmissible between species under certain conditions; however, the efficiency of amyloid deposition is species-specific and appears to be related to the primary amino acid sequence, especially the N-terminal segment of the amyloid protein.
Liu
Y
Y
First Department of Pathology, Yamaguchi University School of Medicine, Ube, Yamaguchi, Japan.
Cui
D
D
Hoshii
Y
Y
Kawano
H
H
Une
Y
Y
Gondo
T
T
Ishihara
T
T
D002612:Acinonyx; D000595:Amino Acid Sequence; D000686:Amyloidosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D000818:Animals; D001717:Birds; D002415:Cats; D002417:Cattle; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D007150:Immunohistochemistry; D008099:Liver / Q000473:pathology; D051379:Mice; D008813:Mice, Inbred ICR; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics; D000685:Serum Amyloid A Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity
D006023:Glycoproteins; D010446:Peptide Fragments; D000685:Serum Amyloid A Protein; C046748:amyloid enhancing factor