The transcription reinitiation properties of RNA polymerase III in the absence of transcription factors.
Transcription reinitiation by RNA polymerase (Pol) III proceeds through facilitated recycling, a process by which the terminating Pol III, assisted by the transcription factors TFIIIB and TFIIIC, rapidly reloads onto the same transcription unit. To get further insight into the Pol III transcription mechanism, we analyzed the kinetics of transcription initiation and reinitiation of a simplified in vitro transcription system consisting only of Pol III and template DNA. The data indicates that, in the absence of transcription factors, first-round transcription initiation by Pol III proceeds at a normal rate, while facilitated reinitiation during subsequent cycles is compromised.
Ferrari
Roberto
R
Dipartimento di Biochimica e Biologia Molecolare, Università degli Studi di Parma, Viale G.P. Usberti 23/A, 43100, Parma, Italy.
Dieci
Giorgio
G
D001483:Base Sequence; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D008969:Molecular Sequence Data; D012320:RNA Polymerase III / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D035361:Transcription Factor TFIIIB / Q000172:deficiency* / Q000502:physiology; D019474:Transcription Factors, TFIII / Q000172:deficiency* / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology*; D000098832:Transcription Factor TFIIIC
D029701:Saccharomyces cerevisiae Proteins; D035361:Transcription Factor TFIIIB; D019474:Transcription Factors, TFIII; D000098832:Transcription Factor TFIIIC; D012320:RNA Polymerase III