title: Miscibility and in vitro osteocompatibility of biodegradable blends of poly[(ethyl alanato) (p-phenyl phenoxy) phosphazene] and poly(lactic acid-glycolic acid).
abstract: Previously we demonstrated the ability of ethyl glycinato substituted polyphosphazenes to neutralize the acidic degradation products and control the degradation rate of poly(lactic acid-glycolic acid) (PLAGA) by blending. In this study, blends of high strength poly[(50% ethyl alanato) (50% p-phenyl phenoxy) phosphazene] (PNEA(50)PhPh(50)) and 85:15 PLAGA were prepared using a mutual solvent approach. Three different solvents, methylene chloride (MC), chloroform (CF) and tetrahydrofuran (THF) were studied to investigate solvent effects on blend miscibility. Three different blends were then fabricated at various weight ratios namely 25:75 (BLEND25), 50:50 (BLEND50), and 75:25 (BLEND75) using THF as the mutual solvent. The miscibility of the blends was evaluated by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and Fourier transform infrared spectroscopy (FTIR). Among these, BLEND25 was miscible while BLEND50 and BLEND75 were partially miscible. Furthermore, BLEND25 formed apatite layers on its surface as evidenced in a biomimetic study performed. These novel blends showed cell adhesion and proliferation comparable to PLAGA. However, the PNEA(50)PhPh(50) component in the blends was able to increase the phenotypic expression and mineralized matrix synthesis of the primary rat osteoblasts (PRO) in vitro. Blends of high strength PNEA(50)PhPh(50) and 85:15 PLAGA are promising biomaterials for a variety of musculoskeletal applications.
authors.affiliation: Department of Chemical Engineering, University of Virginia, Charlottesville, VA 22904, USA.
authors.forename: Meng
authors.initials: M
authors.lastname: Deng
authors.forename: Lakshmi S
authors.initials: LS
authors.lastname: Nair
authors.forename: Syam P
authors.initials: SP
authors.lastname: Nukavarapu
authors.forename: Sangamesh G
authors.initials: SG
authors.lastname: Kumbar
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Krogman
authors.forename: Anurima
authors.initials: A
authors.lastname: Singh
authors.forename: Harry R
authors.initials: HR
authors.lastname: Allcock
authors.forename: Cato T
authors.initials: CT
authors.lastname: Laurencin
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism*; D040761:Biomimetic Materials / Q000737:chemistry / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D019344:Lactic Acid / Q000737:chemistry* / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000737:chemistry* / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism*; D051381:Rats; D012997:Solvents; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D009943:Organophosphorus Compounds; D011091:Polyesters; D011108:Polymers; D012997:Solvents; C527258:poly((ethyl alanato) (p-phenyl phenoxy) phosphazene); D019344:Lactic Acid; C033616:poly(lactide); D000469:Alkaline Phosphatase