title: Antifungal activity of a novel compound from Burkholderia cepacia against plant pathogenic fungi.
abstract: OBJECTIVE
  To investigate antifungal activity of a novel compound (named as CF66I provisionally) against plant pathogenic fungi, mainly including Fusarium sp., Colletotrichum lindemuthianum, Rhizoctonia solani, etc.
  
  
  RESULTS
  Minimal inhibition concentrations (MIC) and minimal fungicidal concentrations (MFC) of CF66I for each fungi were determined using serial broth dilution method. The data demonstrated MIC ranged from 2.5 to 20.0 microg ml(-1) and MFC were shown at levels of < or =7.5 microg ml(-1) except Fusarium sp. With reverse microscopy, profound morphological alterations of fungal cells were observed after exposure to CF66I. Conidiospores were completely inhibited, and protoplasm aggregated to form chalamydospores because of the changes of cell permeability. Some chalamydospores were broken, suggesting the compound probably possessed strong ability of damaging the cell wall. In addition, CF66I was investigated for its antifungal stability against Curvularia lunata. The results showed CF66I kept strong fungi-static activity over-wide pH range (pH 4-9) and temperature range (from -70 to 120 degrees C).
  
  
  CONCLUSIONS
  The compound CF66I exhibited strong and stable broad-spectrum antifungal activity, and had a significant fungicidal effect on fungal cells.
  
  
  CONCLUSIONS
  Results from prebiocontrol evaluations performed to date are probably useful in the search for alternative approaches to controlling serious plant pathogens.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: C-S
authors.initials: CS
authors.lastname: Quan
authors.forename: S-D
authors.initials: SD
authors.lastname: Fan
mesh_terms: D000577:Amides / Q000302:isolation & purification / Q000494:pharmacology*; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D016956:Burkholderia cepacia / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D003904:Mitosporic Fungi / Q000166:cytology / Q000187:drug effects*; D010935:Plant Diseases / Q000382:microbiology*; D013696:Temperature
chemical_list: D000577:Amides; D000935:Antifungal Agents; C525443:CF66I compound