Electrofocusing the compost organic matter obtained by coupling SEC-PAGE.
Humic acids (HA)-like extracted from compost at the beginning (t(0)) and after 130 days of composting (t(130)) were fractionated by coupling size exclusion chromatography to polyacrylamide gel electrophoresis (SEC-PAGE). HA-like fractions with the same molecular size (MS) and electrophoretic mobility were pooled and further characterised by analytical polyacrylamide gel electrofocusing (EF) and compared with HA separated from a Typic Chernozem soil. During the composting process all fractions were subjected to quantitative and qualitative modifications: the high MS fraction was degraded, the mid MS fractions were qualitatively changed, the content of low MS fractions increased and changed qualitatively. The main changes in EF pattern of the non fractionated HA-like t(130) were associated to low MS fractions. Such data seem to be reliable for explanation what mechanisms and monitoring of the evolution of the compost organic matter for their agricultural uses.
Cavani
Luciano
L
Dipartimento di Scienze e Tecnologie Agroambientali, Alma Mater Studiorum, Università di Bologna, Viale Fanin 40, I-40127 Bologna, Italy. lcavani@agrsci.unibo.it
Trubetskaya
Olga
O
Grigatti
Marco
M
Trubetskoj
Oleg
O
Ciavatta
Claudio
C
D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods*; D005591:Chemical Fractionation; D002845:Chromatography / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods*; D004396:Coloring Agents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods; D006812:Humic Substances / Q000032:analysis; D007525:Isoelectric Focusing / Q000379:methods*; D008970:Molecular Weight; D012987:Soil; D012989:Soil Pollutants; D013997:Time Factors
D004396:Coloring Agents; D006812:Humic Substances; D012987:Soil; D012989:Soil Pollutants