Enzymatic activities of uridine and thymidine phosphorylase in normal and cancerous uterine cervical tissues.
In this study, the preliminary analyses were conducted of enzymatic activities of uridine phosphorylase (UP) and thymidine phosphorylase (TP) in normal tissues and cancer tissues of the uterine cervix. The study was performed on 27 patients of cervical cancer, treated first in our hospital. Normal cervical tissues obtained from 15 patients undergoing hysterectomy for benign diseases were used as controls. The supernatant of the homogenated cervical tissues and the stroma (5-FU and ribose-1-P or deoxyribose-1-P) were analyzed by high performance liquid chromatography, and then the UP and TP activities calculated. TP activity was significantly greater than UP activity (P < 0.0001). Both UP and TP showed significantly greater activity in cancer tissues than in normal tissues (P < 0.0001). In the TP activity of the cancer tissues, there was no significant difference among the histological types, while the TP activity tended to be significantly higher in the cases with lymph node metastasis. These results showed that the TP-mediated route seemed important as the 5FU metabolic pathway in the uterine cervical tissues, and TP enzymatic activity might be associated with lymph node metastasis.
Kobayashi
Yoichi
Y
Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kanagawa, Japan. y5koba@marianna-u.ac.jp
Wada
Yasuna
Y
Ohara
Tatsuru
T
Okuda
Yoshiko
Y
Suzuki
Nao
N
Hasegawa
Kiyoshi
K
Kiguchi
Kazushige
K
Ishizuka
Bunpei
B
D000230:Adenocarcinoma / Q000201:enzymology* / Q000556:secondary; D000964:Antimetabolites, Antineoplastic / Q000378:metabolism; D018196:Carcinoma, Adenosquamous / Q000201:enzymology* / Q000556:secondary; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000556:secondary; D002584:Cervix Uteri / Q000201:enzymology*; D005260:Female; D005472:Fluorouracil / Q000378:metabolism; D006801:Humans; D008207:Lymphatic Metastasis; D013939:Thymidine Phosphorylase / Q000378:metabolism*; D014543:Uridine Phosphorylase / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000201:enzymology* / Q000473:pathology
D000964:Antimetabolites, Antineoplastic; D014543:Uridine Phosphorylase; D013939:Thymidine Phosphorylase; D005472:Fluorouracil