title: An E. coli expression system optimized for DELLA proteins.
abstract: The DELLA proteins are involved in regulation of plant growth in response to phytohormonal signals such as GA, ethylene, and auxin. They have become one of most challenging and active area of research due to their fundamental roles in plant biology. Here, we describe the first successful expression of the N-terminal domains of DELLA proteins of Arabidopsis thaliana and Malus domestica in Escherichia coli system which will be used to produce monoclonal antibodies for profiling protein micro-arrays. Optimizations of the cloning, expression, and purification using specific tags have been discussed.
authors.affiliation: The Horticulture and Food Research Institute of New Zealand Ltd., Private Bag 11 030, Palmerston North, New Zealand. xsun@hortresearch.co.nz
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Sun
authors.forename: Nicky
authors.initials: N
authors.lastname: Frearson
authors.forename: Christopher
authors.initials: C
authors.lastname: Kirk
authors.forename: William T
authors.initials: WT
authors.lastname: Jones
authors.forename: Dawn
authors.initials: D
authors.lastname: Harvey
authors.forename: Jasna
authors.initials: J
authors.lastname: Rakonjac
authors.forename: Toshi
authors.initials: T
authors.lastname: Foster
authors.forename: Taha
authors.initials: T
authors.lastname: Al-Samarrai
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005822:Genetic Vectors; D005875:Gibberellins / Q000378:metabolism*; D027845:Malus / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification
chemical_list: D005875:Gibberellins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins