[Comparison of urinary proteomics between steroid-sensitive and steroid-resistant minimal change nephrotic syndrome in children].
OBJECTIVE
To screen the differentially expressed proteins in the urine of children with steroid-sensitive and steroid-resistant minimal change nephrotic syndrome (SRINS and SSINS, respectively).


METHODS
Urine samples were collected from 10 children with SRINS and 70 with SSINS as well as 30 healthy volunteers (control). Isoelectric focusing and two-dimensional electrophoresis in combination with matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry was performed for analysis of the urine proteins.


CONCLUSIONS
In the urine samples, 30 protein spots were identified to have differential expression between SRINS and SSINS. Further analysis of 14 protein spots identified 12 proteins expressing in SRINS, namely kinesin family member 27, PITPNB, bullous pemphigoid antigen, alpha-1 protease inhibitor, Zn-alpha-2GP, alpha-1B-glycoprotein, serum albumin precursor, haptoglobin precursor, kinesin like motor protein, IRAK4, cytoplasmic dynein and cytokeratin 9. Nine of these 12 proteins were up-regulated (U1-U3, U5, U7-U9, U11-U12) and 3 down-regulated (D4, D6, D10) in SRINS, suggesting that these proteins may serve as the potential therapeutic targets and as new diagnostic markers for steroid-resistant nephrotic syndrome.
Huang
Yan-Jun
YJ
Department of Internal Medicine, Children's Hospital, Nanjing Medical University, Nanjing 210029, China. huangyanjundr315@163.com
Huang
Song-Ming
SM
Zhang
Ai-Hua
AH
Zheng
Guo
G
Chen
Rong-Hua
RH
D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D009402:Nephrosis, Lipoid / Q000188:drug therapy* / Q000652:urine*; D011506:Proteins / Q000737:chemistry; D040901:Proteomics; D013256:Steroids / Q000627:therapeutic use*; D014556:Urine / Q000737:chemistry*
D011506:Proteins; D013256:Steroids