Western blot assay using recombinant p26 antigen for detection of equine infectious anemia virus-specific antibodies.
We analyzed the performance of a single-band Western blot (WB) test using recombinant p26 (rp26) capsid protein of equine infectious anemia virus. According to the results obtained, the rp26 WB test is a reliable confirmatory diagnostic tool to be used as a complementary test after an enzyme-linked immunosorbent assay or agar gel immunodiffusion test yielding doubtful results.
Alvarez
I
I
Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, INTA, C.C. 1712, Castelar, Argentina. ialvarez@cnia.inta.gov.ar
Gutierrez
G
G
Ostlund
E
E
Barrandeguy
M
M
Trono
K
K
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D015153:Blotting, Western / Q000379:methods / Q000592:standards / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004926:Escherichia coli / Q000235:genetics; D006736:Horses; D005779:Immunodiffusion / Q000379:methods / Q000592:standards / Q000662:veterinary; D004860:Infectious Anemia Virus, Equine / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D015203:Reproducibility of Results; D012698:Serologic Tests / Q000662:veterinary; D014758:Viral Core Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014758:Viral Core Proteins; C117895:equine infectious anemia virus, p26