[Effect of matrine injections on invasion and metastasis of gastric carcinoma SGC-7901 cells in vitro].
OBJECTIVE
To investigate the effect of matrine injections on invasion and metastasis of gastric carcinoma SGC-7901 cells in vitro.


METHODS
MTT assay was used to examine the effect of matrine injections on proliferation of SGC-7901 cells after 24, 48, 72, 96 hours treatment; Transwell chamber assay was performed to determine the effect of matrine injection on invasion and migratory capacity of the cells; Effect on adhesion potential of SGC-7901 cells was tested by cell matrigel adhesion assay.


RESULTS
Matrine injec-tions could inhibit the proliferation of SGC-7901 cells, with obvious dose-dependent and time-dependent effects. Matrine injections sig-nificantly inhibited adhesion, invasion and migration capacity of SGC-7901 cells in vitro. The inhibitory rate of them after treatment with 1.5 mg/ml matrine injections for 24 h were (45.32 +/- 3.10)%, (32.66 +/- 2.82)%, (38.35 +/- 3.41)% respectively. After treat-ment of matrine injections (1.5 mg/ml)for 24 h, the expression level of CD44(V6) in SGC-7901 cells was decreased compared with the untreated group.


CONCLUSIONS
Matrine injections can inhibit the migration, invasion and adhesion capacity of SGC-7901 cells in vitro. The inhibition effect may be related to down-regulating the expression of CD44(V6) protein.
Dai
Zhi-jun
ZJ
Department of Oncology, The Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. dzj0911@126.com
Gao
Jie
J
Wu
Wen-ying
WY
Wang
Xi-jing
XJ
Li
Zong-fang
ZF
Kang
Hua-feng
HF
Liu
Xiao-xu
XX
Ma
Xiao-bin
XB
D000470:Alkaloids / Q000008:administration & dosage / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D007267:Injections; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010946:Plants, Medicinal / Q000737:chemistry; D011807:Quinolizines / Q000008:administration & dosage / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D000093842:Matrines
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D018960:Hyaluronan Receptors; D011807:Quinolizines; D000093842:Matrines