Analysis of hTERT expression in exfoliated cells from patients with bladder transitional cell carcinomas using SYBR green real-time fluorescence quantitative PCR.
OBJECTIVE
To investigate whether hTERT mRNA expression could be used as a diagnostic and prognostic marker of bladder transitional cell carcinoma.


METHODS
SYBR green I real-time fluorescence quantitative PCR method was used to quantify hTERT mRNA expression in exfoliated cells from patients with bladder transitional cell carcinomas.


RESULTS
A significant correlation was observed between the level of hTERT mRNA expression in exfoliated cells collected in urine with pathological grade and clinical stage.


CONCLUSIONS
Our results show that SYBR green real-time fluorescence quantitative evaluation of hTERT mRNA could be a useful marker of tumor progression for the early diagnosis and prognosis of bladder cancers.
Xie
Xiao-Yan
XY
Department of Urology, Southwest Hospital, Third Military Medical University, Chongqing 400038, PRC.
Yang
Xin
X
Zhang
Jia-Hua
JH
Liu
Ze-Jun
ZJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D052160:Benzothiazoles; D014408:Biomarkers, Tumor / Q000652:urine; D002295:Carcinoma, Transitional Cell / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000652:urine; D002478:Cells, Cultured; D003959:Diamines; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009360:Neoplastic Cells, Circulating / Q000378:metabolism / Q000473:pathology*; D009930:Organic Chemicals; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D011379:Prognosis; D011804:Quinolines; D012333:RNA, Messenger / Q000378:metabolism; D012016:Reference Values; D019098:Telomerase / Q000032:analysis / Q000235:genetics* / Q000652:urine*; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000652:urine
D052160:Benzothiazoles; D014408:Biomarkers, Tumor; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; D012333:RNA, Messenger; C098022:SYBR Green I; C509186:TERT protein, human; D019098:Telomerase