Histological and ultrastructural evaluation of bone around Bio-Oss particles in sinus augmentation.
OBJECTIVE
The aim of the present study was to evaluate histological and ultrastructural features of bone surrounding Bio-Oss particles retrieved, in the same patient, 20 months and 7 years after sinus augmentation.


METHODS
A 54-year patient who needed sinus elevation before implant rehabilitation participated in this study. Two bone cores at two different times were harvested from a Bio-Oss regenerated sinus and processed for examination under light and transmission electron microscopy.


RESULTS
Under light microscopy, in the 20-month specimen, most of the particles were surrounded by a thin layer of newly formed bone; in the 7-year specimen there was mainly compact bone in direct contact with the particles. Under transmission electron microscopy, it was possible to characterize the bone-biomaterial interface; in the 20-month specimen an electron-dense layer was seen, whereas, almost no electron-dense lines were seen at the interface in the 7-year specimen.


CONCLUSIONS
Bio-Oss particles did not interfere with bone-healing processes after sinus augmentation procedures and promoted new bone formation. This study can help clinicians to understand better the morphological characteristics of bone regeneration processes using Bio-Oss after 20 months and, most importantly, after a longer time of interaction with surrounding tissues.
Orsini
G
G
Dental School, University of Chieti-Pescara, Chieti, Italy.
Scarano
A
A
Degidi
M
M
Caputi
S
S
Iezzi
G
G
Piattelli
A
A
D000539:Alveolar Process / Q000648:ultrastructure*; D000540:Alveolar Ridge Augmentation / Q000379:methods*; D018786:Bone Substitutes / Q000627:therapeutic use*; D015921:Dental Implants; D019232:Dental Restoration Failure; D005260:Female; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D008903:Minerals / Q000627:therapeutic use*; D012189:Retrospective Studies; D013997:Time Factors
C077540:Bio-Oss; D018786:Bone Substitutes; D015921:Dental Implants; D008903:Minerals