title: Analysis of hTERT expression in exfoliated cells from patients with bladder transitional cell carcinomas using SYBR green real-time fluorescence quantitative PCR.
abstract: OBJECTIVE
  To investigate whether hTERT mRNA expression could be used as a diagnostic and prognostic marker of bladder transitional cell carcinoma.
  
  
  METHODS
  SYBR green I real-time fluorescence quantitative PCR method was used to quantify hTERT mRNA expression in exfoliated cells from patients with bladder transitional cell carcinomas.
  
  
  RESULTS
  A significant correlation was observed between the level of hTERT mRNA expression in exfoliated cells collected in urine with pathological grade and clinical stage.
  
  
  CONCLUSIONS
  Our results show that SYBR green real-time fluorescence quantitative evaluation of hTERT mRNA could be a useful marker of tumor progression for the early diagnosis and prognosis of bladder cancers.
authors.affiliation: Department of Urology, Southwest Hospital, Third Military Medical University, Chongqing 400038, PRC.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Xie
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.forename: Jia-Hua
authors.initials: JH
authors.lastname: Zhang
authors.forename: Ze-Jun
authors.initials: ZJ
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D052160:Benzothiazoles; D014408:Biomarkers, Tumor / Q000652:urine; D002295:Carcinoma, Transitional Cell / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000652:urine; D002478:Cells, Cultured; D003959:Diamines; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009360:Neoplastic Cells, Circulating / Q000378:metabolism / Q000473:pathology*; D009930:Organic Chemicals; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D011379:Prognosis; D011804:Quinolines; D012333:RNA, Messenger / Q000378:metabolism; D012016:Reference Values; D019098:Telomerase / Q000032:analysis / Q000235:genetics* / Q000652:urine*; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000652:urine
chemical_list: D052160:Benzothiazoles; D014408:Biomarkers, Tumor; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; D012333:RNA, Messenger; C098022:SYBR Green I; C509186:TERT protein, human; D019098:Telomerase