Novel gene-transferring scaffolds having a cell adhesion molecule-DNA-apatite nanocomposite surface.
A low efficiency has long been the most critical problem of conventional gene-transferring systems using calcium phosphates, and this was successfully improved on by using a laminin-DNA-apatite composite (LD-Ap) layer. The gene-transferring efficiency of the LD-Ap surface was 1-2 orders of magnitude higher than that of a DNA-calcium phosphate composite surface. This is because laminin enhances cell adhesion and spreading, and this provides regions of high DNA concentration between a cell and the LD-Ap surface. The efficiency of gene transfer of the LD-Ap surface was equivalent to, or even higher than that mediated using a commercial lipid-based transfection reagent applied using the manufacturer's recommended optimum conditions. In addition, the gene-transferring efficiency of our system could be controlled by changing the laminin and DNA content in the LD-Ap layer. Moreover, our system is composed of highly safe reagents: apatite, DNA and laminin, all of which are present in the human body. Hence, the LD-Ap surface, which enhances cell attachment on its surface, and mediates a safe, highly efficient and controllable gene transfer, is highly applicable to tissue engineering and gene therapy applications.
Oyane
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Nanotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan. a-oyane@aist.go.jp
Tsurushima
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Ito
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D001031:Apatites; D020099:Coated Materials, Biocompatible; D004247:DNA / Q000008:administration & dosage*; D015316:Genetic Therapy; D006801:Humans; D007797:Laminin; D008863:Microspheres; D053761:Nanocomposites / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering; D014162:Transfection / Q000379:methods*
D001031:Apatites; D020099:Coated Materials, Biocompatible; D007797:Laminin; D004247:DNA