title: Establishment and characterization of a new Ewing's sarcoma cell line from a malignant pleural effusion.
abstract: BACKGROUND
  Ewing's sarcoma cell lines may represent a good in vitro model for the understanding of tumor biology in this heterogeneous group of diseases. In the present study, we report the establishment and characterization of a primary Ewing's sarcoma cell line (LDS-Falck 01).
  
  
  METHODS
  LDS-Falck 01 was generated from a malignant pleural effusion of a patient with metastatic peripheral primitive neuroectodermal tumor arising from the chest wall. Extensive characterization of the cells was accomplished using immunocytochemical, RT-PCR and cytogenetic studies.
  
  
  RESULTS
  In vitro LDS-Falck 01 cells had both anchorage-dependent and -independent growth patterns. Immunocytochemical studies showed that cells were PAS-, vimentin-, CD99- and NSE-positive, EGFR- and CD117-negative. Cytogenetic analysis revealed a complex hyperdiploid karyotype with multiple chromosomal aberrations including an unbalanced translocation t(11;22)(q24;q12). The EWS/FLI1 chimeric transcript type 1 was detected.
  
  
  CONCLUSIONS
  This cell line may represent a valid tool for investigating the biomolecular characteristics of this group of neoplasms and their sensitivity to therapeutic agents.
authors.affiliation: Falck Division of Medical Oncology, Ospedale Niguarda Ca' Granda, Milan, Italy. roberta.schiavo@ospedaleniguarda.it
authors.forename: Roberta
authors.initials: R
authors.lastname: Schiavo
authors.forename: Cinzia
authors.initials: C
authors.lastname: Tullio
authors.forename: Mariacarmela
authors.initials: M
authors.lastname: La Grotteria
authors.forename: Ines Chiara
authors.initials: IC
authors.lastname: Andreotti
authors.forename: Barbara
authors.initials: B
authors.lastname: Scarpati
authors.forename: Lorenza
authors.initials: L
authors.lastname: Romiti
authors.forename: Fabio
authors.initials: F
authors.lastname: Bozzi
authors.forename: Paolo
authors.initials: P
authors.lastname: Pedrazzoli
authors.forename: Salvatore
authors.initials: S
authors.lastname: Siena
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016066:Pleural Effusion, Malignant / Q000235:genetics / Q000473:pathology*; D012512:Sarcoma, Ewing / Q000235:genetics / Q000473:pathology*