Gene expression of four adhesive proteins in the early healing of bone defect and bone-implant interface.
The objective of this study was to evaluate the gene expression of four bone-related adhesive proteins during the early healing of bone defect and bone-implant interface in animal experiments. T-shaped hollow pure titanium implants with dual acid-etched surfaces were placed into femurs of 17 Sprague-Dawley rats, and bone defects with the same size were made in the same site in 15 rats. Newly formed bone was harvested at 5 days, 8 days and 16 days respectively. The gene expression of fibronectin (FN), collagen I (COL I), bone sialoprotein II (BSP II) and osteopontin (OPN) in non-implant and bone-implant defects were examined using semi-quantity reverse transcription-polymerase chain reaction. The gene expression of OPN in the non-implant defect was slightly higher than that in the bone-implant interface. At 8 days postoperation, FN, COL I and BSP II expression were significantly up-regulated in the bone-implant group. All four proteins peaked at 8 days. The results indicate that the gene expression of the four adhesive proteins is different between bone defect and bone-implant interface. Intracellular synthesis of FN, COL I and BSP II was accelerated in the early healing stages of the bone-implant interface.
Zhang
Ting
T
Key Lab. of Oral Biomed. Eng., Wuhan Univ. Wuhan 430079, PR China. xhaibin@whu.edu.cn
Xia
Haibin
H
Wang
Yining
Y
Peng
Cong
C
Li
Yuhong
Y
Pan
Xinhua
X
D000818:Animals; D001860:Bone Plates; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005264:Femoral Fractures / Q000503:physiopathology* / Q000601:surgery*; D017102:Fracture Healing / Q000502:physiology*; D005786:Gene Expression Regulation; D008297:Male; D016348:Osseointegration; D051381:Rats; D017207:Rats, Sprague-Dawley
D015815:Cell Adhesion Molecules; D016326:Extracellular Matrix Proteins