title: [Adenovirus-mediated PDCD5 gene transfer sensitizes apoptosis of K562 cells induced by etoposide].
abstract: This study was purposed to investigate the effect of adenovirus-mediated transfer of PDCD5 gene on apoptosis of K562 cells induced by etoposide. Recombinant adenovirus PDCD5 (Ad-PDCD5), control vectors Ad-null and Ad-eGFP were constructed by AdMax vector system respectively. After K562 cells were transfected by Ad-PDCD5, Ad-null or Ad-eGFP with different multiplicity of infection (MOI), the expression level of the PDCD5 gene was examined by RQ-RT-PCR assay. The effects of etoposide in combination with Ad-PDCD5 on the proliferation and apoptosis of K562 cells were measured by using MTT assay and flow cytometry with Annexin-V-FITC/PI dual labeling technique, respectively. The results showed that the transfection efficiencies of Ad-eGFP in K562, Jurkat and CEM cells ranged from 60% to 86%. Expression level of PDCD5 gene in K562 cells was evidently increased following transfection with Ad-PDCD5. The Ad-PDCD5 synergistically enhanced the apoptotic percentage of K562 cells induced by VP-16, as compared with that of Ad-null + VP16 and VP-16 alone respectively. It is concluded that Ad-PDCD5 may be a potential agent for enhancing the chemotherapy effect.
authors.affiliation: Institute of Hematology and People's Hospital, Peking University, Beijing 100044, China.
authors.forename: Guo-Rui
authors.initials: GR
authors.lastname: Ruan
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Chang
authors.forename: Jin-Lan
authors.initials: JL
authors.lastname: Li
authors.forename: Ya-Zhen
authors.initials: YZ
authors.lastname: Qin
authors.forename: Ling-Di
authors.initials: LD
authors.lastname: Li
authors.forename: Le
authors.initials: L
authors.lastname: Hao
authors.forename: Jia-Yu
authors.initials: JY
authors.lastname: Fu
authors.forename: Yan-Rong
authors.initials: YR
authors.lastname: Liu
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Huang
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D005047:Etoposide / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D051017:Apoptosis Regulatory Proteins; D009363:Neoplasm Proteins; C117283:PDCD5 protein, human; D012333:RNA, Messenger; D011994:Recombinant Proteins; D005047:Etoposide