title: Incidence and toxin production ability of Escherichia coli O157:H7 isolated from cattle trucks.
abstract: Twelve cattle trucks were analyzed for the presence of Escherichia coli O157:H7. Three of them had been washed prior to arrival, and the others had not. Seventy-five percent of the trailers were positive for the presence of this foodborne pathogen. A total of 54 cultures were isolated and identified as E. coli O157:H7, all from the trucks that had not been cleaned. Most of the cultures (96.4%) produced Shiga-like toxin (verotoxin). No E. coli O157:H7 was detected in cattle trucks that were cleaned before arrival at the cattle pens. The incidence of E. coli O157:H7 in transport trailers increases the potential risk of contamination of cattle and transmission from farms to feedlots and to packing plants. This contamination increases the potential of contamination of meat during harvest and the risk of foodborne illnesses.
authors.affiliation: Department of Animal Science, Food and Agricultural Products Center, Oklahoma State University, Stillwater, Oklahoma 74078-6055, USA.
authors.forename: E P
authors.initials: EP
authors.lastname: Cuesta Alonso
authors.forename: S E
authors.initials: SE
authors.lastname: Gilliland
authors.forename: C R
authors.initials: CR
authors.lastname: Krehbiel
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D015373:Bacterial Typing Techniques; D002417:Cattle / Q000382:microbiology*; D002418:Cattle Diseases / Q000382:microbiology / Q000635:transmission; D004197:Disease Reservoirs / Q000662:veterinary; D004783:Environmental Microbiology; D004866:Equipment Contamination; D004927:Escherichia coli Infections / Q000382:microbiology / Q000635:transmission / Q000662:veterinary; D019453:Escherichia coli O157 / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D005506:Food Contamination / Q000517:prevention & control; D014186:Transportation
chemical_list: D001427:Bacterial Toxins