Appropriate expression of imprinted genes on mouse chromosome 12 extends development of bi-maternal embryos to term.
Recently, we reported that the restored regulation of imprinted gene expression from two regions -H19 differentially methylated region (H19-DMR) and intergenic germline-derived DMR (IG-DMR) - is sufficient for accomplishing full-term development in mice. In the present study, we determined the developmental ability of the bi-maternal embryos (BMEs) containing the non-growing oocyte genome with the IG-DMR deletion (ng(Deltach12)) and fully-grown (fg) oocyte genome. Foetuses derived from ng(Deltach12)/fg BMEs were alive at E19.5 but could not survive further. Comparison with BMEs derived from Igf2+/- ng/fg genomes suggests that bi-allelic H19 expression might be involved in foetal development.
Kawahara
Manabu
M
Department of BioScience, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan.
Wu
Qiong
Q
Ferguson-Smith
Anne C
AC
Kono
Tomohiro
T
D000818:Animals; D019175:DNA Methylation; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D018392:Genomic Imprinting; D008168:Lung / Q000002:abnormalities; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000378:metabolism; D010641:Phenotype; D011247:Pregnancy; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000235:genetics
C094335:H19 long non-coding RNA; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated