Establishment and characterization of a new Ewing's sarcoma cell line from a malignant pleural effusion.
BACKGROUND
Ewing's sarcoma cell lines may represent a good in vitro model for the understanding of tumor biology in this heterogeneous group of diseases. In the present study, we report the establishment and characterization of a primary Ewing's sarcoma cell line (LDS-Falck 01).


METHODS
LDS-Falck 01 was generated from a malignant pleural effusion of a patient with metastatic peripheral primitive neuroectodermal tumor arising from the chest wall. Extensive characterization of the cells was accomplished using immunocytochemical, RT-PCR and cytogenetic studies.


RESULTS
In vitro LDS-Falck 01 cells had both anchorage-dependent and -independent growth patterns. Immunocytochemical studies showed that cells were PAS-, vimentin-, CD99- and NSE-positive, EGFR- and CD117-negative. Cytogenetic analysis revealed a complex hyperdiploid karyotype with multiple chromosomal aberrations including an unbalanced translocation t(11;22)(q24;q12). The EWS/FLI1 chimeric transcript type 1 was detected.


CONCLUSIONS
This cell line may represent a valid tool for investigating the biomolecular characteristics of this group of neoplasms and their sensitivity to therapeutic agents.
Schiavo
Roberta
R
Falck Division of Medical Oncology, Ospedale Niguarda Ca' Granda, Milan, Italy. roberta.schiavo@ospedaleniguarda.it
Tullio
Cinzia
C
La Grotteria
Mariacarmela
M
Andreotti
Ines Chiara
IC
Scarpati
Barbara
B
Romiti
Lorenza
L
Bozzi
Fabio
F
Pedrazzoli
Paolo
P
Siena
Salvatore
S
D045744:Cell Line, Tumor; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016066:Pleural Effusion, Malignant / Q000235:genetics / Q000473:pathology*; D012512:Sarcoma, Ewing / Q000235:genetics / Q000473:pathology*