Evaluation of the changes of immune cells during lipopolysaccharide-induced mastitis in rats.
The aim of this study was to evaluate in rats, changes in peripheral blood immune cells and mammary tissue after an intramammary infusion of lipopolysaccharide (LPS). The results of the study showed that infusion of LPS induced a rapid migration of neutrophils (PMNs) from the blood to mammary alveoli, increased the activity of myeloperoxidase (MPO) and the concentration of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and interleukin-6 (IL-6) in mammary tissues, decreased the activity of myeloperoxidase in serum and reduced the CD4+/CD8+ ratio. This is the first report of changes in peripheral blood immune cells and mammary tissue in rat mastitis.
Miao
Jin-Feng
JF
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Zhu
Yu-Min
YM
Gu
Bei-Bei
BB
Wang
Xiao-Bin
XB
Zou
Si-Xiang
SX
Deng
Yue-E
YE
D000818:Animals; D016516:CD4-CD8 Ratio; D005260:Female; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity*; D008321:Mammary Glands, Animal / Q000378:metabolism* / Q000473:pathology; D008413:Mastitis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D020556:Neutrophil Infiltration / Q000187:drug effects*; D009504:Neutrophils / Q000378:metabolism* / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D009195:Peroxidase