The chiral resolution of pesticides on amylose-tris(3,5-dimethylphenylcarbamate) CSP by HPLC and the enantiomeric identification by circular dichroism.
Amylose-tris(3,5-dimethylphenylcarbamate) (ADMPC) was synthesized and coated on gamma-aminopropylsilica to prepare a chiral stationary phase (CSP). The chiral resolutions of seven pesticide enantiomers including fenoxaprop-ethyl, quizalofop-ethyl, lactofen, metalaxyl, benalaxyl, hexythiazox and fluroxypyr-meptyl on the CSP by high-performance liquid chromatography were performed. Mobile phase was n-hexane and isopropanol with a flow rate of 1.0 ml/min. The influences of isopropanol content in the mobile phase and temperature on the resolutions were investigated. Under the optimized conditions the enantiomers could obtain complete resolutions except that metalaxyl got partial resolution. Decreasing the content of isopropanol increased the retention and the resolutions. Temperature was an important chromatographic parameter for optimization, and the results showed that low temperature was not always good to the resolutions. The enantiomers were identified by a circular dichroism (CD) detector which could provide the CD signals [(+) or (-)] and the CD spectra in the range of 220-420 nm by online scanning.
Wang
Peng
P
Department of Applied Chemistry, China Agricultural University, Beijing, China.
Liu
Donghui
D
Jiang
Shuren
S
Xu
Yangguang
Y
Gu
Xu
X
Zhou
Zhiqiang
Z
D000688:Amylose / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D010575:Pesticides / Q000737:chemistry*; D048448:Phenylcarbamates / Q000737:chemistry*; D013237:Stereoisomerism; D013696:Temperature
D010575:Pesticides; D048448:Phenylcarbamates; D000688:Amylose