title: Mass spectrometric identification of molecular species of phosphatidylcholine and lysophosphatidylcholine extracted from shark liver.
abstract: The profile and structural characterization of molecular species of phosphatidylcholine (PC) and lysophosphatidylcholine (LysoPC) from shark liver using liquid chromatographic/electrospray ionization mass spectrometry (LC-ESI/MS) and tandem mass spectrometry (MS/MS) are described for the first time in this paper. The presence of (i) a relatively high content of ether PC species, such as 1-O-alkyl- and 1-alk-1'-enyl-2-polyunsaturated PC species (about 20%), and (ii) a high percentage of docosahexaenoic acid (DHA)-containing LysoPC (about 27%) is the characteristic of this marine material. 1-Hexadecanoyl-2-docosahexaenoyl-PC (16:0/22:6; about 24%) and 1-docosahexaenoyl-2-hydroxyl-LysoPC (22:6; about 27%) are the two most abundant species in shark liver. The other polyunsaturated PC species including ether PC are tentatively identified as 1-heptadecanoyl-2-docosahexaenoyl-PC (17:0/22:6), 1-octadecyl-2-docosahexaenoyl-PC (alkyl-18:0/22:6), 1-hexadecyl-2-docosahexaenoyl-PC (alkyl-16:0/22:6), 1-octadecenyl-2-eicosapentaenoyl-PC (alkyl-18:1/20:5), 1-octadecenyl-2-eicosatetraenoyl-PC (alkyl-18:1/20:4), 1-hexadecyl-2-docosapentaenoyl-PC (alkyl-16:0/22:5), 1-(1 Z-hexadecenyl)-2-docosahexaenoyl-PC (alkenyl-16:0/22:6), and 1-octadecenoyl-2-docosahexaenoyl-PC (18:1/22:6). These results establish that high contents of ether DHA-PC and DHA-LysoPC species can be obtained from shark liver.
authors.affiliation: Chainon Neurotrophin Biotechnology Inc., 41 Bayberry Drive, Malta, New York 12020, USA. su@chainonbio.com
authors.forename: Su
authors.initials: S
authors.lastname: Chen
authors.forename: Ka Wan
authors.initials: KW
authors.lastname: Li
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D004281:Docosahexaenoic Acids / Q000032:analysis; D008099:Liver / Q000737:chemistry*; D008244:Lysophosphatidylcholines / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry; D010713:Phosphatidylcholines / Q000032:analysis* / Q000737:chemistry; D012754:Sharks; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D004281:Docosahexaenoic Acids