Enzyme-catalyzed phase transition of alginate gels and gelatin-alginate interpenetrated networks.
The enzyme-catalyzed gel-sol transition of calcium-alginate obtained by internal gelling strategy with the help of an entrapped alginate lyase is described. We show that alginate molecules and enzyme-produced oligoalginates shorten the gel time of physical gelatin gels (5% and 1.5%), probably due to local protein concentration increase. Interpenetrated networks composed of calcium-alginate and of gelatin were obtained only if elongation of gelatin helices inside a pre-existing calcium-alginate network could occur and only for low gelatin concentration (1.5%). The physical gelatin network is almost reversible inside the alginate one. Both networks can be obtained in the presence of alginate lyase, but gel-sol transition of calcium-alginate cannot be obtained in the presence of gelatin.
Doumèche
Bastien
B
Laboratoire ERRMECe UFR Sciences et Techniques Université de Cergy-Pontoise, 2 avenue Adolphe Chauvin, Pontoise cedex, France.
Picard
Julien
J
Larreta-Garde
Véronique
V
D000464:Alginates / Q000737:chemistry* / Q000378:metabolism; D002118:Calcium / Q000737:chemistry; D002384:Catalysis; D004548:Elasticity; D005417:Flavobacterium / Q000201:enzymology; D005780:Gelatin / Q000737:chemistry* / Q000378:metabolism*; D005782:Gels / Q000737:chemistry / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry / Q000378:metabolism; D006603:Hexuronic Acids / Q000737:chemistry / Q000378:metabolism; D044662:Macrocystis / Q000378:metabolism; D015394:Molecular Structure; D044367:Phase Transition; D011133:Polysaccharide-Lyases / Q000378:metabolism; D012212:Rheology; D014783:Viscosity
D000464:Alginates; D005782:Gels; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D005780:Gelatin; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase; D002118:Calcium