title: Production of D-phenylglycine-related amino acids by immobilized microbial cells.
abstract: Bacterial dihydropyrimidinase was shown to catalyze the hydrolytic cleavage of various 5-substituted hydantoins to the corresponding N-carbamyl-D-amino acids under alkaline conditions. Therefore, an enzymatic method for preparing the D-forms of phenylglycine-related amino acids was developed using immobilized bacterial cells with high enzyme activity. Alkalophilic bacteria were a good enzyme source for this process. The process is simple and economical for use in the production of various amino acids with the D-configuration.
authors.affiliation: Department of Agricultural Chemistry, Kyoto University, Kyoto, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamada
authors.forename: S
authors.initials: S
authors.lastname: Shimizu
authors.forename: H
authors.initials: H
authors.lastname: Shimada
authors.forename: Y
authors.initials: Y
authors.lastname: Tani
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Ohashi
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000581:Amidohydrolases / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry; D001407:Bacillus / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology; D001671:Biochemistry / Q000379:methods*; D002384:Catalysis; D018914:Cells, Immobilized; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry; D006827:Hydantoins; D006868:Hydrolysis; D008956:Models, Chemical; D013379:Substrate Specificity; D014508:Urea / Q000031:analogs & derivatives / Q000737:chemistry; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000581:Amidohydrolases; D000596:Amino Acids; D005998:Glycine; D006827:Hydantoins; D014508:Urea; D000099166:Polyacrylamides; C011271:hydantoic acid; C008852:2-phenylglycine; C020047:dihydropyrimidinase