Electrophysiological consequence of adipose-derived stem cell transplantation in infarcted porcine myocardium.
OBJECTIVE
Aim of this study was to investigate the effect of intracoronary administration of freshly isolated adipose-derived mononuclear cells (ADMCs) on myocardial vulnerability to arrhythmia induction after infarction.


RESULTS
A transmural myocardial infarction in an experimental porcine model was induced by occlusion of the mid-left anterior descending artery with an angioplasty balloon for 3 h. Upon reperfusion, a cellular suspension with freshly isolated ADMCs (1.5 x 10(6) cells/kg BW) or vehicle alone was injected into the infarct artery. All animals underwent a programmed ventricular stimulation at 8 weeks follow-up for possible induction of ventricular arrhythmias using a train of 8 S1 stimuli. Cell injections did not cause acute ventricular arrhythmia, bradycardia, or conduction block. The cycle length of the ventricular arrhythmia was compared at 1 and 10 s following its induction. Despite comparable infarct size in both groups, we found that the cycle length of the induced ventricular arrhythmia in the ADMC-treated group was significantly longer compared with control animals (P < 0.05). We also found that extra-stimuli were required for arrhythmia induction in the ADMC-treated group compared with control animals.


CONCLUSIONS
Freshly isolated autologous stem cell therapy is not proarrhythmic in pigs.
Fotuhi
Parwis
P
Department of Medicine, Section of Cardiology, Tulane University Health Sciences Center, New Orleans, LA 70112, USA.
Song
Yao-Hua
YH
Alt
Eckhard
E
D017667:Adipocytes / Q000637:transplantation*; D000818:Animals; D054849:Cardiac Electrophysiology; D004195:Disease Models, Animal; D004562:Electrocardiography; D009203:Myocardial Infarction / Q000503:physiopathology / Q000601:surgery*; D011897:Random Allocation; D012307:Risk Factors; D033581:Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods*; D013552:Swine; D014693:Ventricular Fibrillation / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*