title: Lack of Thy1 (CD90) expression in neuroblastomas is correlated with impaired survival.
abstract: Neuroblastoma (NBL) is the most common solid tumor in children. Tumors in advanced stage or with positive risk factors still have a poor prognosis. Thy1 (CD90) is a membrane glycoprotein expressed in thymus, retinal ganglionic cells, and several types of stem cells. The aim of this study was to assess Thy1 expression in NBL and analyze the correlation with clinical outcome. Sixty-three specimens of NBL were stained for Thy1 on a tissue microarray by immunohistochemistry. Fresh frozen tumor tissues were used for RNA isolation, and RT-PCR analysis for Thy1-mRNA expression was performed. Patients' survival data were correlated with Thy1 status using a log rank test and a Cox regression multivariate analysis. Thy1 was expressed on 51 (81%) of the tumors. Kaplan-Meier survival analysis showed a significantly impaired survival in patients with NBL missing Thy1 (P < 0.005 by log-rank test). A multivariate Cox regression showed an independent prognostic value of Thy1 status for overall survival (P < 0.05). In addition, the frequency of events and deaths was significantly higher in the group of patients with Thy1 negative tumors, as assessed by ANOVA analysis (P < 0.05 by F-test). The data showed that Thy1-negative NBL patients have a significantly impaired overall survival compared with Thy1-positive NBL patients. Thus, Thy1 seemed to be a marker with a specific prognostic value in NBL patients. Future studies are aiming at the biological role of this marker in the tumor cell differentiation.
authors.affiliation: Department of Pediatric Surgery, University of Leipzig, Liebigstrasse 20 A, Leipzig, Germany. henning.fiegel@medizin.uni-leipzig.de
authors.forename: Henning C
authors.initials: HC
authors.lastname: Fiegel
authors.forename: Jussuf T
authors.initials: JT
authors.lastname: Kaifi
authors.forename: Alexander
authors.initials: A
authors.lastname: Quaas
authors.forename: Emine
authors.initials: E
authors.lastname: Varol
authors.forename: Annika
authors.initials: A
authors.lastname: Krickhahn
authors.forename: Roman
authors.initials: R
authors.lastname: Metzger
authors.forename: Guido
authors.initials: G
authors.lastname: Sauter
authors.forename: Holger
authors.initials: H
authors.lastname: Till
authors.forename: Jakob R
authors.initials: JR
authors.lastname: Izbicki
authors.forename: Rudolf
authors.initials: R
authors.lastname: Erttmann
authors.forename: Dietrich
authors.initials: D
authors.lastname: Kluth
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D002675:Child, Preschool; D004587:Electrophoresis, Agar Gel; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D005858:Germany / Q000453:epidemiology; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D009367:Neoplasm Staging; D009447:Neuroblastoma / Q000235:genetics* / Q000378:metabolism / Q000401:mortality; D012334:RNA, Neoplasm / Q000235:genetics*; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate / Q000639:trends; D018800:Thy-1 Antigens / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D012334:RNA, Neoplasm; D018800:Thy-1 Antigens