Resveratrol inhibits TNF-alpha-induced changes of adipokines in 3T3-L1 adipocytes.
Tumor necrosis factor-alpha (TNF-alpha) is chronically elevated in adipose tissues of obese rodents and humans. Increased levels of TNF-alpha are implicated in the induction of atherogenic adipokines, such as plasminogen activator inhibitor -1 (PAI-1) and IL-6, and the inhibition of the anti-atherogenic adipokine, adiponectin. In this study, we investigated the effects of resveratrol on TNF-alpha-induced atherogenic changes of the adipokines in 3T3-L1 cells. Exposure to TNF-alpha for 24 h increased PAI-1 and IL-6 secretion and decreased adiponectin secretion. The mRNA expression of adipokines changed in parallel with mRNA expression. Resveratrol effectively reversed the secretion and mRNA expression of the atherogenic adipokines, PAI-1 and IL-6, induced by TNF-alpha. Decreased secretion levels and mRNA expression of adiponectin by TNF-alpha were also recovered by resveratrol treatment. Our results suggest that resveratrol may improve obesity-induced cardiovascular disease, particularly atherosclerosis, by attenuating the TNF-alpha-induced changes of adipokines.
Ahn
Jiyun
J
Food Function Research Group, Korea Food Research Institute, 516, Baekhyun-dong, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Republic of Korea.
Lee
Hyunjung
H
Kim
Suna
S
Ha
Taeyoul
T
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D052242:Adiponectin / Q000378:metabolism*; D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D015850:Interleukin-6 / Q000378:metabolism*; D051379:Mice; D000077185:Resveratrol; D058258:Serpin E2; D015843:Serpins / Q000378:metabolism*; D013267:Stilbenes / Q000008:administration & dosage*; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage*; D014665:Vasodilator Agents / Q000008:administration & dosage
D052242:Adiponectin; D016207:Cytokines; D015850:Interleukin-6; D058258:Serpin E2; C511869:Serpine2 protein, mouse; D015843:Serpins; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; D014665:Vasodilator Agents; D000077185:Resveratrol