title: Diagnostic reverse-transcription polymerase chain reaction kit for filoviruses based on the strain collections of all European biosafety level 4 laboratories.
abstract: A network of European biosafety level 4 laboratories has designed the first industry-standard molecular assay for all filoviruses species, based on the strain collections of all participants. It uses 5 optimized L gene primers and 3 probes, as well as an internal control with a separate detection probe. Detection limits (probit analysis, 95% detection chance) were as follows: Zaire ebolavirus, 487 copies/mL of plasma; Sudan ebolavirus Maleo, 586 copies/mL; Sudan ebolavirus Gulu, 1128 copies/mL; Cote d'Ivoire ebolavirus, 537 copies/mL; Reston ebolavirus, 4546 copies/mL; Lake Victoria marburgvirus Musoke, 860 copies/mL; and Lake Victoria marburgvirus Ravn, 1551 copies/mL. The assay facilitates reliable detection or exclusion screening of filovirus infections.
authors.affiliation: Bernhard-Nocht Institute for Tropical Medicine, Hamburg 20359, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Panning
authors.forename: Thomas
authors.initials: T
authors.lastname: Laue
authors.forename: Stephan
authors.initials: S
authors.lastname: Olschlager
authors.forename: Markus
authors.initials: M
authors.lastname: Eickmann
authors.forename: Stephan
authors.initials: S
authors.lastname: Becker
authors.forename: Sabine
authors.initials: S
authors.lastname: Raith
authors.forename: Marie-Claude Georges
authors.initials: MC
authors.lastname: Courbot
authors.forename: Mikael
authors.initials: M
authors.lastname: Nilsson
authors.forename: Robin
authors.initials: R
authors.lastname: Gopal
authors.forename: Ake
authors.initials: A
authors.lastname: Lundkvist
authors.forename: Antonino
authors.initials: A
authors.lastname: di Caro
authors.forename: David
authors.initials: D
authors.lastname: Brown
authors.forename: Hermann
authors.initials: H
authors.lastname: Meyer
authors.forename: Graham
authors.initials: G
authors.lastname: Lloyd
authors.forename: Beate M
authors.initials: BM
authors.lastname: Kummerer
authors.forename: Stephan
authors.initials: S
authors.lastname: Gunther
authors.forename: Christian
authors.initials: C
authors.lastname: Drosten
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D017931:DNA Primers; D005060:Europe; D016563:Filoviridae / Q000235:genetics / Q000302:isolation & purification*; D018702:Filoviridae Infections / Q000517:prevention & control*; D000882:Haplorhini; D006801:Humans; D007753:Laboratories / Q000592:standards*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012449:Safety; D012680:Sensitivity and Specificity; D014709:Vero Cells
chemical_list: D017931:DNA Primers; D011933:Reagent Kits, Diagnostic