An Na+/H+ antiporter gene from wheat plays an important role in stress tolerance.
A vacuole Na+/H+ antiporter gene TaNHX2 was obtained by screening the wheat cDNA library and by the 5'-RACE method. The expression of TaNHX2 was induced in roots and leaves by treatment with NaCl, polyethylene glycol (PEG), cold and abscisic acid (ABA). When expressed in a yeast mutant (deltanhx1), TaNHX2 suppressed the salt sensitivity of the mutant,which was deficient in vacuolar Na+/H+ antiporter, and caused partial recovery of growth of delta nhx1 in NaCl and LiCl media. The survival rate of yeast cells was improved by overexpressing the TaNHX2 gene under NaCl, KCl, sorbitol and freezing stresses when compared with the control. The results imply that TaNHX2 might play an important role in salt and osmotic stress tolerance in plant cells.
Yu
Jia Ning
JN
National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Huang
Jian
J
Wang
Zi Ning
ZN
Zhang
Jin Song
JS
Chen
Shou Yi
SY
D000595:Amino Acid Sequence; D027682:Cation Transport Proteins / Q000096:biosynthesis / Q000235:genetics; D003080:Cold Temperature; D003470:Culture Media; D005615:Freezing; D018021:Lithium Chloride; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D018384:Oxidative Stress / Q000235:genetics* / Q000502:physiology; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics; D012965:Sodium Chloride; D017923:Sodium-Hydrogen Exchangers / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D013012:Sorbitol; D014908:Triticum / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D014617:Vacuoles / Q000235:genetics / Q000502:physiology
D027682:Cation Transport Proteins; D003470:Culture Media; C492183:NHX1 protein, S cerevisiae; D010940:Plant Proteins; D029701:Saccharomyces cerevisiae Proteins; D017923:Sodium-Hydrogen Exchangers; D012965:Sodium Chloride; D013012:Sorbitol; D018021:Lithium Chloride