title: Discovery of small-molecule inhibitors of tyrosinase.
abstract: To identify novel inhibitors of tyrosinase, a fluorescent assay was developed which is suitable for high-throughput screening. In the assay, oxidation of the substrate by tyrosinase leads to the release of a fluorescent coumarin. Several small molecules were identified that inhibited mushroom tyrosinase in vitro and human tyrosinase in cell culture. These compounds may represent lead structures for therapies targeted at disorders of hyperpigmentation.
authors.affiliation: Department of Dermatology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9038, USA.
authors.forename: Juris P
authors.initials: JP
authors.lastname: Germanas
authors.forename: Shugauang
authors.initials: S
authors.lastname: Wang
authors.forename: Andrew
authors.initials: A
authors.lastname: Miner
authors.forename: Wayne
authors.initials: W
authors.lastname: Hao
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Ready
mesh_terms: D000363:Agaricales / Q000187:drug effects / Q000201:enzymology; D002384:Catalysis; D002460:Cell Line; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D015394:Molecular Structure; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010084:Oxidation-Reduction; D010858:Pigmentation / Q000187:drug effects; D013329:Structure-Activity Relationship
chemical_list: D004791:Enzyme Inhibitors; D014442:Monophenol Monooxygenase