[Construction of 3D complex of porous beta-tricalcium phosphate/collagen scaffolds and dog periodontal ligament cells].
OBJECTIVE
To investigate the construction of 3D complex of porous beta-tricalcium phosphate/collagen scaffolds (beta-TCP/col) and dog periodontal ligament cells (PDLCs).


METHODS
Dog PDLCs were isolated, cultured and identified. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate the cytotoxicity of beta-TCP/col on the proliferation of PDLCs. The cells were seeded onto porous beta-TCP/col scaffolds. The cellular capability of adhesion and growth on porous beta-TCP/col surface was investigated visually by scanning electron microscopy (SEM).


RESULTS
The cytotoxicity assay indicated that there was no significant difference between beta-TCP/col and the control during the 7 days (P>0.05). SEM showed cells successfully adhered to porous beta-TCP/col scaffolds and spread extensively. Matrix secretions were found on the cell surface.


CONCLUSIONS
Porous beta-tricalcium phosphate/collagen scaffolds were of good biocompatibility to the dog periodontal ligament cells, and were potential ideal candidates for periodontal tissue engineering.
Zhong
Jin-sheng
JS
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Ouyang
Xiang-ying
XY
Mei
Fang
F
Deng
Xu-liang
XL
Cao
Cai-fang
CF
D000818:Animals; D001672:Biocompatible Materials; D002130:Calcium Phosphates; D002478:Cells, Cultured; D003094:Collagen; D003764:Dental Materials; D004285:Dogs; D010513:Periodontal Ligament / Q000166:cytology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D003764:Dental Materials; C485817:beta-tricalcium phosphate; D003094:Collagen