title: Small-scale protein production with the baculovirus expression vector system.
abstract: Improved methods of baculovirus cloning and insect cell culture and their commercialization have made the use of the baculovirus expression vector system (BEVS) a routine tool for the production of preparative quantities of recombinant protein. This chapter outlines basic techniques for small-scale protein production using the BEVS, including protocols for expression from adherent and suspension insect cell cultures, titer estimation, and expression optimization.
authors.affiliation: Molecular Probes Invitrogen Detection Technologies, Eugene, OR, USA.
authors.forename: Steve
authors.initials: S
authors.lastname: Harwood
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D005822:Genetic Vectors / Q000235:genetics*; D007313:Insecta; D008967:Molecular Biology / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins