Expression of a human cytomegalovirus latency-associated homolog of interleukin-10 during the productive phase of infection.
The human cytomegalovirus UL111A region is active during both productive and latent phases of infection. During productive infection, the virus expresses ORF79, a protein with oncogenic properties, and cmvIL-10, a functional homolog of human IL-10. During latent infection of myeloid progenitor cells, an alternately spliced variant of cmvIL-10, termed latency-associated (LA) cmvIL-10 has previously been identified. To determine whether LAcmvIL-10 transcription occurs during productive infection, we performed 5' and 3' RACE to map UL111A-region transcripts in productively infected human foreskin fibroblasts (HFFs). This analysis revealed the presence of a singly spliced UL111A-region transcript predicted to encode LAcmvIL-10. This transcript was expressed in HFFs with early (beta) kinetics, a temporal class that differs from that of ORF79 (alpha kinetics) and cmvIL-10 (gamma kinetics). These data identify and map a transcript encoding a latency-associated homolog of IL-10 which is expressed by the virus during the productive phase of infection.
Jenkins
Christina
C
Centre for Virus Research, Westmead Millennium Institute and University of Sydney, New South Wales, Australia.
Garcia
Winnie
W
Abendroth
Allison
A
Slobedman
Barry
B
D001483:Base Sequence; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000235:genetics* / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000235:genetics / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*; D017735:Virus Latency / Q000235:genetics
D017931:DNA Primers; D004279:DNA, Viral; C508609:IL10 protein, human; D014764:Viral Proteins; D016753:Interleukin-10