[Enhancement of Fas-mediated apoptosis in leukemic cell line HL-60 by Bay 11 - 7082].
The aim of study was to explore the effects of NF-kappaB inhibitor Bay 11 - 7082 on Fas/FasL system and Fas-mediated apoptosis in HL-60 cells. The mRNA and protein expression levels of Fas, FasL and XIAP after treatment with Bay 11 - 7082 were detected by RT-PCR and FCM respectively. The level of sFasL was detected by ELISA before and after treatment with Bay 11 - 7082; apoptosis was detected by FCM before and after treatment with Bay 11 - 7082. The results showed that after treating HL-60 cells with Bay 11 - 7082, the mRNA and protein levels of FasL and XIAP were lower than that of controls, the difference was significant by statistic analysis (p < 0.05). Neither the mRNA and protein levels of Fas, nor the level of sFasL changed significantly (p > 0.05). Apoptotic rate of HL-60 cells treated with Bay 11 - 7082 was significantly higher as compared with controls (p < 0.05). It is concluded that Bay 11 - 7082 can enhance Fas-mediated apoptosis in HL-60 cells by down-regulation of FasL and XIAP levels.
Wang
Li
L
Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of science and technology, Wuhan 430022, China.
Liu
Ling-Bo
LB
Li
Lei
L
Zou
Ping
P
D017209:Apoptosis / Q000187:drug effects*; D015536:Down-Regulation; D053222:Fas Ligand Protein / Q000502:physiology*; D015973:Gene Expression Regulation, Leukemic; D018922:HL-60 Cells; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D009570:Nitriles / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D013450:Sulfones / Q000494:pharmacology*; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000378:metabolism*; D019014:fas Receptor / Q000378:metabolism*
C434003:3-(4-methylphenylsulfonyl)-2-propenenitrile; C491901:FAS protein, human; D053222:Fas Ligand Protein; D016328:NF-kappa B; D009570:Nitriles; D012333:RNA, Messenger; D013450:Sulfones; D051636:X-Linked Inhibitor of Apoptosis Protein; D019014:fas Receptor