Comparison of the proximal promoter regions of the PAX3 and PAX7 genes.
Translocations t(2;13)(q35;q14) and t(1;13)(p36;q14), which fuse PAX3 and PAX7, respectively, to FOXO1A, characterize alveolar rhabdomyosarcoma. Previous studies have suggested that the expression of PAX7-FOXO1A is copy-number dependent, but that of PAX3-FOXO1A is not, which may be due to a weaker PAX7 than PAX3 promoter. The aim of the present study was to compare the transcriptional activities of the PAX3 and PAX7 proximal promoter regions, using the dual-luciferase reporter assay with three vector systems in eight cell lines. The PAX3 promoter was found to have higher transcriptional activity than that of PAX7 irrespective of the vector system or cell line used. These findings are consistent with the idea that an amplification event is required for the PAX7-FOXO1A chimeric transcript to reach a critical expression level.
Möller
Emely
E
Department of Clinical Genetics, Lund University Hospital, Getingevägen 4, 221 85, Lund, Sweden. emely.moller@med.lu.se
Isaksson
Margareth
M
Mandahl
Nils
N
Mertens
Fredrik
F
Panagopoulos
Ioannis
I
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D002648:Child; D002869:Chromosome Aberrations; D017931:DNA Primers; D005784:Gene Amplification; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D000072041:PAX3 Transcription Factor; D051763:PAX7 Transcription Factor / Q000235:genetics*; D051761:Paired Box Transcription Factors / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012208:Rhabdomyosarcoma / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection; D014178:Translocation, Genetic
D017931:DNA Primers; D000072041:PAX3 Transcription Factor; C489409:PAX3 protein, human; D051763:PAX7 Transcription Factor; C497321:PAX7 protein, human; D051761:Paired Box Transcription Factors