[Development of multiplex real-time PCR assay for the detection of herpes simplex virus types 1 and 2].
We developed a multiplex real-time PCR assay to simultaneously detect herpes simplex virus-1 (HSV-1) and HSV-2 genomes. TaqMan PCR primer pairs and fluologenic probes targeting the HSV-1 or HSV-2 gpD region were originally designed. The detection limit was 15 copies/tube, and the quantitative range was from 15 to 1.5 x 10(6) copies/tube of HSV-1 or HSV-2 DNA. The sensitivity of this assay was 10(3) times higher than the conventional PCR assay. No other herpes virus DNA-Cytomegalovirus, Epstein-Barr virus, human herpes virus 6, human herpes virus 7, or varicella-zoster virus-were detected by this assay. These results indicated that the multiplex real-time PCR assay is useful for rapidly diagnosing HSV-1 and HSV-2.
Nagashima
Mami
M
Department of Microbiology, Tokyo Metropolitan Institute of Public Health.
Sadamasu
Kenji
K
Shinkai
Takayuki
T
Yoshida
Yasuko
Y
Yamada
Sumio
S
D003199:Computer Systems; D004279:DNA, Viral / Q000032:analysis; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*
D004279:DNA, Viral