title: [Interleukin-8 expression in lung tissue during ischemia-reperfusion].
abstract: OBJECTIVE
  Local cytokine production is a pathogenic factor in ischemia-reperfusion injury in early graft dysfunction. This study analyzed interleukin 8 (IL-8) messenger RNA (mRNA) expression in lung tissue and the association between IL-8 mRNA levels and interstitial lung changes in an experimental model of warm lung ischemia-reperfusion.
  
  
  METHODS
  We studied 16 New Zealand rabbits divided into 3 groups: control, ischemia (tissue taken from right lower lobe after 1, 2, or 3 hours of ischemia), and reperfusion (tissue taken from right upper and middle lobes after 1 hour of ischemia and 1, 2, or 3 hours of reperfusion). Expression of IL-8 mRNA was determined by reverse transcription and polymerase chain reaction. Interstitial infiltration by polymorphonuclear neutrophils was determined. The Mann-Whitney U-test was used for statistical comparisons, with P< .05 considered to indicate a significant result.
  
  
  RESULTS
  During ischemia, IL-8 mRNA levels were elevated at the end of hour 1 (P=.009) with respect to the control group, but not thereafter. Interstitial changes were minimal. IL-8 mRNA levels during reperfusion were similar to those observed during ischemia, with a slight increase at the end of hour 2. There were no significant differences between hours 1, 2, and 3. Polymorphonuclear neutrophil recruitment occurred at the beginning of reperfusion (P=.014), but no significant differences were observed at hours 2 or 3. Progressive thickening of alveolar septa and edema was documented.
  
  
  CONCLUSIONS
  Changes in IL-8 mRNA expression during ischemia precede interstitial infiltration by polymorphonuclear neutrophils during reperfusion, suggesting that the 2 processes are related. Quantification of IL-8 mRNA expression could facilitate early diagnosis of graft dysfunction.
authors.affiliation: Unidad de Cirugía Torácica Experimental, Facultad de Medicina, Universidad de Valladolid, Valladolid, España. jmmatilla17@hotmail.com
authors.forename: José María
authors.initials: JM
authors.lastname: Matilla
authors.forename: Mariano
authors.initials: M
authors.lastname: García Yuste
authors.forename: Mariano
authors.initials: M
authors.lastname: Sánchez Crespo
authors.forename: Manuel J
authors.initials: MJ
authors.lastname: Gayoso
authors.forename: Félix
authors.initials: F
authors.lastname: Heras
authors.forename: Marta
authors.initials: M
authors.lastname: Jiménez Prada
authors.forename: Andrés
authors.initials: A
authors.lastname: Alonso
authors.forename: Guillermo
authors.initials: G
authors.lastname: Ramos
mesh_terms: D000818:Animals; D016209:Interleukin-8 / Q000032:analysis / Q000096:biosynthesis*; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D009504:Neutrophils; D011817:Rabbits; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology
chemical_list: D016209:Interleukin-8