title: Expression of E-cadherin, beta-catenin and Ki-67 antigen and their reciprocal relationships in mammary adenocarcinomas in bitches.
abstract: In progression of tumours, resulting from, i.e., release of cells from the parental tumour and development of metastases, expression of cell adhesion molecules (CAM) plays a significant role. CAM, including E-cadherin and the linked to it beta-catenin, determine the extent of adhesion between normal and neoplastically altered cells. Moreover, the unbound form of beta-catenin in a cell nucleus may affect the rate of cell proliferation This study aimed at demonstrating intensity and localisation of E-cadherin and beta-catenin expression as related to expression of the proliferation-associated antigen, Ki-67 in mammary adenocarcinomas of bitches. The study was performed on 35 cases of the above mentioned tumours. On paraffin sections immunohistochemical reactions were performed using monoclonal antibodies directed against E-cadherin, beta-catenin and Ki-67 antigen. In the studies a membranous expression of E-cadherin, a cytoplasmic-nuclear expression of beta-catenin and nuclear expression of Ki-67 antigen were demonstrated. Statistical calculations using Spearman's test demonstrated a pronounced positive correlation between expression of beta-catenin and Ki-67 antigen and absence of correlation between expression of E-cadherin and Ki-67 antigen. No correlation could be detected between expression intensities of E-cadherin and beta-catenin.
authors.affiliation: Chair of Pathomorphology, Pathophysiology, Microbiology and Forensic Veterinary Science, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland. martin75@ozi.ar.wroc.pl
authors.forename: Marcin
authors.initials: M
authors.lastname: Nowak
authors.forename: Janusz A
authors.initials: JA
authors.lastname: Madej
authors.forename: Piotr
authors.initials: P
authors.lastname: Dziegiel
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D015820:Cadherins / Q000096:biosynthesis*; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D015674:Mammary Neoplasms, Animal / Q000378:metabolism* / Q000473:pathology; D051176:beta Catenin / Q000096:biosynthesis*
chemical_list: D015820:Cadherins; D019394:Ki-67 Antigen; D051176:beta Catenin