title: Revisiting TRPC1 and TRPC6 mechanosensitivity.
abstract: This article addresses whether TRPC1 or TRPC6 is an essential component of a mammalian stretch-activated mechano-sensitive Ca(2+) permeable cation channel (MscCa). We have transiently expressed TRPC1 and TRPC6 in African green monkey kidney (COS) or Chinese hamster ovary (CHO) cells and monitored the activity of the stretch-activated channels using a fast pressure clamp system. Although both TRPC1 and TRPC6 are highly expressed at the protein level, the amplitude of the mechano-sensitive current is not significantly altered by overexpression of these subunits. In conclusion, although several TRPC channel members, including TRPC1 and TRPC6, have been recently proposed to form MscCa in vertebrate cells, the functional expression of these TRPC subunits in heterologous systems remains problematic.
authors.affiliation: Single Molecule Biophysics, SUNY, 301 Cary Hall, Buffalo, NY 14214, USA.
authors.forename: Philip
authors.initials: P
authors.lastname: Gottlieb
authors.forename: Joost
authors.initials: J
authors.lastname: Folgering
authors.forename: Rosario
authors.initials: R
authors.lastname: Maroto
authors.forename: Albert
authors.initials: A
authors.lastname: Raso
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Wood
authors.forename: Alex
authors.initials: A
authors.lastname: Kurosky
authors.forename: Charles
authors.initials: C
authors.lastname: Bowman
authors.forename: Delphine
authors.initials: D
authors.lastname: Bichet
authors.forename: Amanda
authors.initials: A
authors.lastname: Patel
authors.forename: Frederick
authors.initials: F
authors.lastname: Sachs
authors.forename: Boris
authors.initials: B
authors.lastname: Martinac
authors.forename: Owen P
authors.initials: OP
authors.lastname: Hamill
authors.forename: Eric
authors.initials: E
authors.lastname: Honoré
mesh_terms: D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D008465:Mechanoreceptors / Q000502:physiology*; D018613:Microscopy, Confocal; D018408:Patch-Clamp Techniques; D010957:Plasmids; D050052:TRPC Cation Channels / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D000074287:TRPC6 Cation Channel; D014162:Transfection
chemical_list: D050052:TRPC Cation Channels; D000074287:TRPC6 Cation Channel; C117032:TRPC6 protein, human; C096351:transient receptor potential cation channel, subfamily C, member 1