Noninvasive assessment of the role of cyclooxygenases in cardiovascular health: a detailed HPLC/MS/MS method.
A robust method for the routine quantitation of a selected group of urinary eicosanoid metabolites of interest to cardiovascular research in human and mouse is described and discussed. Included are the addition of stable isotope-labeled internal standards, solid phase extraction, and quantitation by liquid chromatography/tandem mass spectrometry using selected reaction monitoring (SRM) techniques.
Song
Wen-Liang
WL
Institute for Translational Medicine and Therapeutics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Lawson
John A
JA
Wang
Miao
M
Zou
Helen
H
FitzGerald
Garret A
GA
D000818:Animals; D015415:Biomarkers / Q000378:metabolism / Q000652:urine; D002318:Cardiovascular Diseases / Q000201:enzymology / Q000652:urine; D002319:Cardiovascular System / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015777:Eicosanoids / Q000378:metabolism* / Q000592:standards / Q000652:urine; D006801:Humans; D051379:Mice; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000379:methods
D015415:Biomarkers; D015777:Eicosanoids; D011451:Prostaglandin-Endoperoxide Synthases