title: [Solubilization of components of outer membrane of Leptospira interrogans serovar mozdok by sodium desoxycholate and triton X-100 and evaluation of their immunogenic capacity and homologous and heterologous protection induced in hamsters].
abstract: Two detergents (triton X-100 and sodium desoxycholate) were evaluated as solubilizing agents of the components of the outer membrane of Leptospira Pomona serovar mozdok. It was obtained a greater yielding of proteins on applying the treatment with triton X-100. The variants formulated starting from the extracts obtained showed a high immunogenicity and capacity of homologous protection against the challenge in hamster.
authors.affiliation: Laboratorio de Bioquímica, Instituto Finlay. yofernandez@finlay.edu.cu
authors.forename: Yovania Fernández
authors.initials: YF
authors.lastname: Rivera
authors.forename: Yadira Pino
authors.initials: YP
authors.lastname: Navarro
authors.forename: Yisabel Aranguren
authors.initials: YA
authors.lastname: Mazorra
authors.forename: Andrés González
authors.initials: AG
authors.lastname: Rodríguez
authors.forename: María de los Angeles
authors.initials: Mde L
authors.lastname: Padrón
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Valdés
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D006224:Cricetinae; D003840:Deoxycholic Acid / Q000494:pharmacology*; D004195:Disease Models, Animal; D007921:Leptospira interrogans / Q000276:immunology* / Q000472:pathogenicity; D041341:Leptospira interrogans serovar canicola / Q000276:immunology; D007922:Leptospirosis / Q000276:immunology / Q000517:prevention & control*; D007928:Lethal Dose 50; D017830:Octoxynol / Q000494:pharmacology*
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D003840:Deoxycholic Acid; D017830:Octoxynol