Proton-dependent transporter gene lndJ confers resistance to landomycin E in Streptomyces globisporus.
Sequence analysis of 2 kb BamHI-SmaI fragment of landomycin E (LaE) gene cluster in S. globisporus 1912 revealed one complete ORF marked as lndJ. Analysis of putative LndJ aminoacid sequence allowed us to suppose that it is proton-dependent antiporter which could be involved in resistance to LaE in the producing strain. Although disruption of lndJ had no significant influence on LaE production and resistance, it's overexpression in wild type and LaE overproducing strains led to qualitative changes in landomycins biosynthesis and increased resistance to LaE. These data support the hypothesis about involvement of lndJ gene in landomycins export.
Ostash
I
I
Department of Genetics and Biotechnology, Ivan Franko National University of L'viv, 79005 L'viv, Ukraine.
Ostash
B
B
Walker
S
S
Fedorenko
V
V
D000617:Aminoglycosides / Q000378:metabolism / Q000494:pharmacology*; D017920:Antiporters / Q000235:genetics / Q000502:physiology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000502:physiology; D013302:Streptomyces / Q000187:drug effects* / Q000235:genetics*
D000617:Aminoglycosides; D017920:Antiporters; C113593:landomycin E