[Expressions of fibronectin and bone morphogenetic protein-2, 4 during development of mouse tooth germ].
OBJECTIVE
The purpose of this study was to investigate the distribution and relationship of fibronectin (FN), bone morphogenetic protein (BMP) 2, 4 during development of mouse tooth germ.


METHODS
Rat embryonic first molars were collected from E14 (bud stage) and E18 (bell stage). The expressions of FN and BMP-2, 4 were analyzed with immunohistochemistry.


RESULTS
FN was located in the epithelium and dental mesenchyme on bud stage, but on bell stage, the FN was found at the region of differentiating odontoblasts and in the inner enamel epithelium, and also BMP-2, 4 were abundant mainly at the brisk region of differentiating odontoblasts.


CONCLUSIONS
The results demonstrate that BMP-2, 4 and FN play the important roles during bud stage and bell stage, and there may be synergy between BMP-2, 4 and FN in regulating differentiation and maturity of odontoblasts.
Zhang
Jian
J
Department of Oral and Maxillofacial Surgery, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Tian
Wei-dong
WD
Nie
Xin
X
Liu
Lei
L
Tang
Wei
W
Lin
Yun-feng
YF
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D055415:Bone Morphogenetic Protein 4 / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D051381:Rats; D017207:Rats, Sprague-Dawley; D014083:Tooth Germ / Q000196:embryology*
C525720:Bmp2 protein, rat; C525811:Bmp4 protein, rat; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D005353:Fibronectins