[Influence of repeated seizures and large dosage anti-epileptic drug on phosphorylated cAMP response element binding protein in rat's hippocampus and effect of Caoguo Zhimu Decoction].
OBJECTIVE
To study the influence of repeated seizures and anti-epileptic drug on phosphorylated cAMP response element binding protein (pCREB) in rat model of cognitive impairment, and the effect of Caoguo Zhimu Decoction (CZD) on it.


METHODS
On the basis of epileptic model induced by pentylenetetrazol (PTZ), cognitive impairment model was induced by kindling epilepsy with PTZ everyday, which were then di-vided into the model-1 group, the CZD-1 group, the nimodipine-1 group, and those by injecting large dosage phenytoin sodium were divided into the model-2 group, the CZD-2 group and the nimodipine-2 group. Changes of pCREB protein in rat's hippocampus were detected using immunohistochemistry and Western blotting assay.


RESULTS
The expression of pCREB was higher in the CZD-1 and nimodipine-1 group than in the model group, while it was significantly different in the CZD-2 and nimodipine-2 groups than in the model-2 group.


CONCLUSIONS
CZD could relieve the cognitive dysfunction in the epileptic model rats induced by everyday PTZ kindling or by dilantin through increasing the pCREB expression.
He
Juan
J
Beijing University of Chinese Medicine. hejuan64282@sina.com
Ma
Shi-Lei
SL
D000818:Animals; D003072:Cognition Disorders / Q000139:chemically induced / Q000517:prevention & control*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D004827:Epilepsy / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007696:Kindling, Neurologic / Q000187:drug effects; D008297:Male; D010433:Pentylenetetrazole; D010672:Phenytoin / Q000009:adverse effects; D010766:Phosphorylation; D008517:Phytotherapy; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D017362:Cyclic AMP Response Element-Binding Protein; D004365:Drugs, Chinese Herbal; C533633:caoguo zhimu; D010672:Phenytoin; D010433:Pentylenetetrazole