title: Comparative proteome analysis of neural retinas from type 2 diabetic rats by two-dimensional electrophoresis.
abstract: OBJECTIVE
  The purpose of this study was to isolate, identify, and analyze diabetes-related protein changes that occur in neural retinas in vivo.
  
  
  METHODS
  Total proteins were extracted from neural retinas of normal and 8-weeks diabetic Sprague-Dawley (SD) rats and separated by two-dimensional gel electrophoresis (2-DE). Some protein spots exhibiting statistically significant variations (p < 0.05) were selected randomly and identified by mass spectrometry (MS or MS/MS). The protein alphaA-crystallin was chosen as a target for specific immunodetection using Western blot to corroborate the variation found by 2-DE.
  
  
  RESULTS
  Twenty protein spots identified included alphaA-crystallin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), glutamine (Gln) synthetase, and so forth. Western blotting analyses confirmed that alphaA-crystallin protein expression was upregulated in diabetic retina.
  
  
  CONCLUSIONS
  In this study, we isolate, identify, and analyze diabetes-related protein changes that occur in neural retinas in vivo. Further investigation of candidate proteins may identify novel pharmacological targets for diabetic retinopathy.
authors.affiliation: Department of Ophthalmology, First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Ya-Dong
authors.initials: YD
authors.lastname: Wang
authors.forename: Jin-Dao
authors.initials: JD
authors.lastname: Wu
authors.forename: Zhong-Li
authors.initials: ZL
authors.lastname: Jiang
authors.forename: Yu-bang
authors.initials: YB
authors.lastname: Wang
authors.forename: Xue-Hao
authors.initials: XH
authors.lastname: Wang
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Ming-Qing
authors.initials: MQ
authors.lastname: Tong
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000503:physiopathology; D004041:Dietary Fats / Q000008:administration & dosage; D015180:Electrophoresis, Gel, Two-Dimensional; D005136:Eye Proteins / Q000378:metabolism; D007328:Insulin / Q000097:blood; D008297:Male; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood; D038202:alpha-Crystallin A Chain / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D004041:Dietary Fats; D005136:Eye Proteins; D007328:Insulin; D014280:Triglycerides; D038202:alpha-Crystallin A Chain; D002784:Cholesterol