title: Can we build synthetic, multicellular systems by controlling developmental signaling in space and time?
abstract: Using biological machinery to make new, functional molecules is an exciting area in chemical biology. Complex molecules containing both 'natural' and 'unnatural' components are made by processes ranging from enzymatic catalysis to the combination of molecular biology with chemical tools. Here, we discuss applying this approach to the next level of biological complexity -- building synthetic, functional biotic systems by manipulating biological machinery responsible for development of multicellular organisms. We describe recent advances enabling this approach, including first, recent developmental biology progress unraveling the pathways and molecules involved in development and pattern formation; second, emergence of microfluidic tools for delivering stimuli to a developing organism with exceptional control in space and time; third, the development of molecular and synthetic biology toolsets for redesigning or de novo engineering of signaling networks; and fourth, biological systems that are especially amendable to this approach.
authors.affiliation: Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637, USA. r-ismagilov@uchicago.edu
authors.forename: Rustem F
authors.initials: RF
authors.lastname: Ismagilov
authors.forename: Michel M
authors.initials: MM
authors.lastname: Maharbiz
mesh_terms: D015509:Developmental Biology / Q000379:methods*; D015202:Protein Engineering; D015398:Signal Transduction