Detection of high-risk papillomavirus DNA with commercial invader-technology-based analyte-specific reagents following automated extraction of DNA from cervical brushings in ThinPrep media.
We compared the performances of the Third Wave Technology Invader method and the Digene Hybrid Capture 2 assay to detect high-risk human papillomaviruses in 87 cervical brushing specimens submitted in Cytyc ThinPrep media. Two different methods for the extraction of DNA from squamous epithelial cells were also evaluated.
Schutzbank
Ted E
TE
CompuNet Clinical Laboratories, Moraine, OH, USA. ted.e.schutzbank@questdiagnostics.com
Jarvis
Charlene
C
Kahmann
Nicole
N
Lopez
Katherine
K
Weimer
Marlea
M
Yount
Aleta
A
D002584:Cervix Uteri / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000821:virology*; D011933:Reagent Kits, Diagnostic
D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic