[Interleukin-8 expression in lung tissue during ischemia-reperfusion].
OBJECTIVE
Local cytokine production is a pathogenic factor in ischemia-reperfusion injury in early graft dysfunction. This study analyzed interleukin 8 (IL-8) messenger RNA (mRNA) expression in lung tissue and the association between IL-8 mRNA levels and interstitial lung changes in an experimental model of warm lung ischemia-reperfusion.


METHODS
We studied 16 New Zealand rabbits divided into 3 groups: control, ischemia (tissue taken from right lower lobe after 1, 2, or 3 hours of ischemia), and reperfusion (tissue taken from right upper and middle lobes after 1 hour of ischemia and 1, 2, or 3 hours of reperfusion). Expression of IL-8 mRNA was determined by reverse transcription and polymerase chain reaction. Interstitial infiltration by polymorphonuclear neutrophils was determined. The Mann-Whitney U-test was used for statistical comparisons, with P< .05 considered to indicate a significant result.


RESULTS
During ischemia, IL-8 mRNA levels were elevated at the end of hour 1 (P=.009) with respect to the control group, but not thereafter. Interstitial changes were minimal. IL-8 mRNA levels during reperfusion were similar to those observed during ischemia, with a slight increase at the end of hour 2. There were no significant differences between hours 1, 2, and 3. Polymorphonuclear neutrophil recruitment occurred at the beginning of reperfusion (P=.014), but no significant differences were observed at hours 2 or 3. Progressive thickening of alveolar septa and edema was documented.


CONCLUSIONS
Changes in IL-8 mRNA expression during ischemia precede interstitial infiltration by polymorphonuclear neutrophils during reperfusion, suggesting that the 2 processes are related. Quantification of IL-8 mRNA expression could facilitate early diagnosis of graft dysfunction.
Matilla
José María
JM
Unidad de Cirugía Torácica Experimental, Facultad de Medicina, Universidad de Valladolid, Valladolid, España. jmmatilla17@hotmail.com
García Yuste
Mariano
M
Sánchez Crespo
Mariano
M
Gayoso
Manuel J
MJ
Heras
Félix
F
Jiménez Prada
Marta
M
Alonso
Andrés
A
Ramos
Guillermo
G
D000818:Animals; D016209:Interleukin-8 / Q000032:analysis / Q000096:biosynthesis*; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D009504:Neutrophils; D011817:Rabbits; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology
D016209:Interleukin-8