title: [Effects of lead exposure on expression of mGluR5 in mRNA and protein levels of the cultured hippocampal neurons].
abstract: OBJECTIVE
  To study the effects on the expression of mGluR5 mRNA and protein levels in primarily cultured hippocampal neurons after lead exposure.
  
  
  METHODS
  Primary embryonic rat hippocampal neuronal culture was prepared. On the 3(rd) day of incubation, lead chloride solution was added into medium to produce four different lead exposure levels: 0, 1 x 10(-8) mol/L, 1 x 10(-6) mol/L, 1 x 10(-4) mol/L Pb(2+). After 10 days of incubation, the neurons were collected to measure the alteration of mGluR5 mRNA expression by real-time fluorescent quantity PCR and the expression of mGluR5 in protein level by Western blot.
  
  
  RESULTS
  The studies revealed that mGluR5 mRNA expression was down-regulated after lead exposure in a dose-dependent manner. The mGluR5 mRNA expression of the lower lead-exposed neurons (Pb(2+) 10(-8) mol/L), the medium lead-exposed neurons(Pb(2+) 10(-6) mol/L), the higher lead-exposed neurons(Pb(2+) 10(-4) mol/L) were 0.724, 0.421, 0.321 times less than that of the controls, respectively. The Western blot demonstrated that mGluR5 expression in protein level should be decreased after lead exposure.
  
  
  CONCLUSIONS
  The expression of mGluR5 in mRNA and protein levels should be down-regulated after lead exposure at different lead levels in a dose-dependent manner.
authors.affiliation: Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Institute for Pediatric Research, Shanghai Key Laboratory of Children's Environmental Health, Shanghai 200092, China.
authors.forename: Han-fang
authors.initials: HF
authors.lastname: Xie
authors.forename: Chong-huai
authors.initials: CH
authors.lastname: Yan
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: Yu
authors.initials: Y
authors.lastname: Gao
authors.forename: Xiao-gang
authors.initials: XG
authors.lastname: Yu
authors.forename: Jun
authors.initials: J
authors.lastname: Pan
authors.forename: Xiao-dan
authors.initials: XD
authors.lastname: Yu
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Shen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005260:Female; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007854:Lead / Q000633:toxicity*; D020263:Lead Poisoning, Nervous System / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C578937:Grm5 protein, rat; D012333:RNA, Messenger; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate; D007854:Lead