Identification of human cytomegalovirus UL84 virus- and cell-encoded binding partners by using proteomics analysis.
Human cytomegalovirus (HCMV) UL84 is a phosphoprotein that shuttles from the nucleus to the cytoplasm and is required for oriLyt-dependent DNA replication and viral growth. UL84 was previously shown to interact with IE2 (IE86) in infected cells, and this interaction down-regulates IE2-mediated transcriptional activation in transient assays. UL84 and IE2 were also shown to cooperatively activate a promoter within HCMV oriLyt. UL84 alone can interact with an RNA stem-loop within oriLyt and is bound to this structure within the virion. In an effort to investigate the binding partners for UL84 in infected cells, we pulled down UL84 from protein lysates prepared from HCMV-infected human fibroblasts by using a UL84-specific antibody and resolved the immunoprecipitated protein complexes by two-dimensional gel electrophoresis. We subsequently identified individual proteins by matrix-assisted laser desorption ionization-tandem time of flight analysis. This analysis revealed that UL84 interacts with viral proteins UL44, pp65, and IE2. In addition, a number of cell-encoded proteins were identified, including ubiquitin-conjugating enzyme E2, casein kinase II (CKII), and the multifunctional protein p32. We also confirmed the interaction between UL84 and IE2 as well as the interaction of UL84 with importin alpha. UL44, pp65, and CKII interactions were confirmed to occur in infected and cotransfected cells by coimmunoprecipitation assays followed by Western blotting. Ubiquitination of UL84 occurred in the presence and absence of the proteasome activity inhibitor MG132 in infected cells. The identification of UL84 binding partners is a significant step toward the understanding of the function of this significant replication protein.
Gao
Yang
Y
University of Nevada-Reno, Department of Microbiology, Howard Bldg. 210, Reno, NV 89557, USA.
Colletti
Kelly
K
Pari
Gregory S
GS
D047390:Casein Kinase II / Q000302:isolation & purification / Q000378:metabolism; D002460:Cell Line; D003587:Cytomegalovirus / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005347:Fibroblasts / Q000737:chemistry / Q000821:virology; D006801:Humans; D017874:Immediate-Early Proteins / Q000302:isolation & purification / Q000378:metabolism; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D020543:Proteome / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015534:Trans-Activators / Q000302:isolation & purification / Q000378:metabolism; D044763:Ubiquitin-Conjugating Enzymes / Q000302:isolation & purification / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000302:isolation & purification / Q000378:metabolism; D014764:Viral Proteins / Q000302:isolation & purification / Q000378:metabolism*
D004268:DNA-Binding Proteins; C072217:ICP36 protein, Cytomegalovirus; C069186:IE2 protein, Cytomegalovirus; D017874:Immediate-Early Proteins; D010750:Phosphoproteins; D020543:Proteome; D015534:Trans-Activators; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C065418:cytomegalovirus matrix protein 65kDa; C072218:UL84 protein, Cytomegalovirus; D044763:Ubiquitin-Conjugating Enzymes; D047390:Casein Kinase II