title: Mimotopes identify conformational B-cell epitopes on the two major house dust mite allergens Der p 1 and Der p 2.
abstract: House dust mite allergy occurs in 10-20% of the population. Improvement of the present immunotherapy requires detailed knowledge about the structure of the allergens. Mimotopes selected from phage peptide libraries imitate the conformational epitopes of a natural allergen. The aim of our study was to generate epitope mimics for the two major allergens of house dust mite. When the monoclonal anti-Der p 1 and anti-Der p 2 antibodies were used for biopannings, mimotopes were selected which bound also specific IgE from human allergic patients' sera. The conformational matching of these mimotopes on the 3D structure of the natural allergens determined discontinuous epitopes in both cases, representing conformational B-cell epitopes relevant for binding of human IgE. Therefore, these mimotopes are potential candidates for the directed induction of blocking antibodies and epitope-specific immunotherapy of mite allergy.
authors.affiliation: Department of Pathophysiology, Center of Physiology, Pathophysiology and Immunology, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Szalai
authors.forename: Jan
authors.initials: J
authors.lastname: Fuhrmann
authors.forename: Tea
authors.initials: T
authors.lastname: Pavkov
authors.forename: Michael
authors.initials: M
authors.lastname: Scheidl
authors.forename: Julia
authors.initials: J
authors.lastname: Wallmann
authors.forename: Kira H
authors.initials: KH
authors.lastname: Brämswig
authors.forename: Susanne
authors.initials: S
authors.lastname: Vrtala
authors.forename: Otto
authors.initials: O
authors.lastname: Scheiner
authors.forename: Walter
authors.initials: W
authors.lastname: Keller
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Saint-Remy
authors.forename: Dirk
authors.initials: D
authors.lastname: Neumann
authors.forename: Isabella
authors.initials: I
authors.lastname: Pali-Schöll
authors.forename: Erika
authors.initials: E
authors.lastname: Jensen-Jarolim
mesh_terms: D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D060829:Arthropod Proteins; D003546:Cysteine Endopeptidases; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000628:therapy; D007073:Immunoglobulin E / Q000378:metabolism; D007167:Immunotherapy; D018716:Molecular Mimicry; D019151:Peptide Library; D011487:Protein Conformation
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C451163:Dermatophagoides pteronyssinus antigen p 2; D018985:Epitopes, B-Lymphocyte; D019151:Peptide Library; D007073:Immunoglobulin E; D003546:Cysteine Endopeptidases; C451162:Dermatophagoides pteronyssinus antigen p 1