title: Novel production method and in-vitro cell compatibility of porous Ti-6Al-4V alloy disk for hard tissue engineering.
abstract: Porous metals are attractive due to its unique physical, mechanical, and new bone tissue ingrowth properties. In the present study, the production of highly porous Ti-6Al-4V parts by powder metallurgical technology and subsequently it's uses in in vitro bone tissue engineering is described. A space-holder method using carbamide with different particle size to produce parts with porosities between 35 and 70% were applied. The compressive strength and Young's modulus of porous Ti-6Al-4V were determined. Results indicated that stress and Young's modulus decrease with increasing porosity and pore size. The porous parts are characterized by scanning electron microscopy. Furthermore, study was to investigate the effects of three different porosities of porous Ti-6Al-4V (35, 50, and 70%) on proliferation, differentiation, and cell-matrix interaction of mouse osteoblast-like cells, MC-3T3. Results showed that the cell proliferation was significantly (p < 0.05) higher on 70% porous Ti-6Al-4V. However, synthesis of different types of extra cellular matrix proteins was also more abundant on 70% porous Ti-6Al-4V than 35 and 50% porous Ti-6Al-4V disk except some specific proteins. An increase in alkaline phosphate activity was significantly (p < 0.05) higher on 70 and 50% porous Ti-6Al-4V disk after 12 days of MC-3T3 cells incubation. Above all, results indicated that porosity (nearly 70%) of porous Ti-6Al-4V topography affects proliferation and differentiation of osteoblast-like MC-3T3 cells. The results showed that this novel process is a promise to fabricate porous biomaterials for bone implants.
authors.affiliation: Department of Bionanosystem Engineering, Chonbuk National University, Chonju 561-756, Republic of Korea.
authors.forename: Shanta Raj
authors.initials: SR
authors.lastname: Bhattarai
authors.forename: Khalil Abdel-Razek
authors.initials: KA
authors.lastname: Khalil
authors.forename: Montasser
authors.initials: M
authors.lastname: Dewidar
authors.forename: Pyoung Han
authors.initials: PH
authors.lastname: Hwang
authors.forename: Ho Keun
authors.initials: HK
authors.lastname: Yi
authors.forename: Hak Yong
authors.initials: HY
authors.lastname: Kim
mesh_terms: D016475:3T3 Cells; D000497:Alloys / Q000737:chemistry / Q000627:therapeutic use*; D000535:Aluminum; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000592:standards; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D006244:Hardness; D008422:Materials Testing; D019563:Mechanics; D008722:Methods; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D014025:Titanium
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D000535:Aluminum; D014025:Titanium