Serum protein patterns in newly diagnosed type 2 diabetes mellitus--influence of diabetic environment and family history of diabetes.
BACKGROUND
Individuals with normal glucose tolerance (NGT) and type 2 diabetes mellitus (T2DM) represent heterogeneous groups with differences in beta-cell function and genetic background. The aim of the present study was to compare serum protein profiles of NGT and T2DM individuals and determine the influence of the genetic background versus diabetic environment on differentially displayed proteins.


METHODS
Surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) was used to compare serum protein profiles of NGT persons and T2DM patients. All participants were from the Stockholm Diabetes Prevention Program (SDPP) cohort. They were selected to have high or low beta-cell function (HOMA-beta) and family history of type 2 diabetes (FHD) or not.


RESULTS
Eight proteins were found to be elevated and five lowered (p<0.05) in serum of T2DM patients. In a second comparison, the NGT and T2DM groups were divided into persons with FHD and low HOMA-beta and those without FHD and high HOMA-beta. Three proteins were rediscovered and interpreted to be different due to genetic background. Two of these were identified as apolipoprotein C3 (apoC3) and albumin. Ten proteins were interpreted to be not related to FHD, and one of these was identified as transthyretin.


CONCLUSIONS
Using the SELDI-technique, serum protein profiles of NGT and T2DM persons with differences in beta-cell function and FHD were compared. The diabetic environment had a major influence on most of these proteins, while FHD was an important factor for apoC3 and albumin.
Sundsten
Tea
T
Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden. Tea.Sundsten@mcb.uu.se
Ostenson
Claes-Göran
CG
Bergsten
Peter
P
D000328:Adult; D053305:Apolipoprotein C-III / Q000097:blood; D001798:Blood Proteins / Q000737:chemistry*; D015331:Cohort Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D004777:Environment; D005192:Family Health; D005951:Glucose Tolerance Test; D006801:Humans; D050417:Insulin-Secreting Cells / Q000502:physiology; D008297:Male; D008875:Middle Aged; D011228:Prealbumin / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D053305:Apolipoprotein C-III; D001798:Blood Proteins; D011228:Prealbumin; D012709:Serum Albumin