title: Production of fructooligosaccharides by crude enzyme preparations of beta-fructofuranosidase from Aureobasidium pullulans.
abstract: Fructooligosaccharides (FOS) were produced from sucrose by using crude enzyme preparations of beta-fructofuranosidases (FFases) obtained from sucrose-cultured cells of Aureobasidium pullulans DSM 2404. When the preparation mainly consisted of FFase I, that has high transfructosylating activity, the FOS yield was 62%. When the reaction was carried out with additional commercial glucose isomerase (GI) at an activity ratio of FFase and GI of 1:2, the maximum FOS yield reached 69%. This value was higher than those obtained previously using other Aureobasidium spp. (53-59%).
authors.affiliation: Department of Advanced Bioresources Science, Graduate School of Science and Technology, Chiba University, Matsudo-City, Chiba, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Yoshikawa
authors.forename: Seigo
authors.initials: S
authors.lastname: Amachi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Shinoyama
authors.forename: Takaaki
authors.initials: T
authors.lastname: Fujii
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000378:metabolism*; D001203:Ascomycota / Q000201:enzymology* / Q000254:growth & development; D009844:Oligosaccharides / Q000096:biosynthesis*; D043324:beta-Fructofuranosidase / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; C116580:fructooligosaccharide; D043324:beta-Fructofuranosidase; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase