Characterization of protein-protein interactions: application to the understanding of peroxisome biogenesis.
With the approaching completion of the Pichia pastoris genome, a greater emphasis will have to be placed on the proteome and the protein-protein interactions between its constituents. This chapter discusses methods that have been used for the study of such interactions among both soluble and membrane-associated proteins in peroxisome biogenesis. The procedures are equally applicable to other cellular processes.
Leon
Sebastien
S
Section of Molecular Biology, University of California San Diego, La Jolla, CA, USA.
Suriapranata
Ivet
I
Yan
Mingda
M
Rayapuram
Naganand
N
Patel
Amar
A
Subramani
Suresh
S
D003432:Cross-Linking Reagents / Q000494:pharmacology; D005656:Fungal Proteins / Q000378:metabolism*; D047468:Immunoprecipitation; D020675:Peroxisomes / Q000187:drug effects / Q000378:metabolism*; D010843:Pichia / Q000187:drug effects / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects
D003432:Cross-Linking Reagents; D005656:Fungal Proteins