title: Marker profiling of normal keratinocytes identifies the stroma from squamous cell carcinoma of the oral cavity as a modulatory microenvironment in co-culture.
abstract: OBJECTIVE
  The microenvironment established by stromal cells may or may not influence phenotypic aspects of epithelial cells and may be relevant for tumor and stem cell biology. We address this issue for keratinocytes using tumor-derived stromal cells in a co-culture system.
  
  
  METHODS
  We isolated stromal cells from human squamous cell carcinoma tissue and studied their effect on phenotypic characteristics of normal human interfollicular keratinocytes in vitro.
  
  
  RESULTS
  Stromal fibroblasts significantly influence immuno- and lectin cytochemical properties of co-cultured normal keratinocytes. Expression of keratins 8 and 19, the nucleolar protein nucleostemin, parameters related to adhesion/growth-regulatory galectins and the epithelial-mesenchymal transition were altered. This biological activity of tumor-derived stromal cells, which did not require cell contact, appeared to be stable, because it was maintained during passaging of keratinocytes in the absence of cancer cells.
  
  
  CONCLUSIONS
  Tumor-derived stromal fibroblasts acquire distinct properties to shape a microenvironment conducive to altering the phenotypic characteristics of normal epithelial cells in vitro.
authors.affiliation: First Faculty of Medicine, Institute of Anatomy, Charles University in Prague, Prague, Czech Republic.
authors.forename: Lukas
authors.initials: L
authors.lastname: Lacina
authors.forename: Barbora
authors.initials: B
authors.lastname: Dvoránkova
authors.forename: Karel
authors.initials: K
authors.lastname: Smetana
authors.forename: Martin
authors.initials: M
authors.lastname: Chovanec
authors.forename: Jan
authors.initials: J
authors.lastname: Plzák
authors.forename: Ruth
authors.initials: R
authors.lastname: Tachezy
authors.forename: Linda
authors.initials: L
authors.lastname: Kideryová
authors.forename: L
authors.initials: L
authors.lastname: Kucerová
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Cada
authors.forename: Jan
authors.initials: J
authors.lastname: Boucek
authors.forename: R
authors.initials: R
authors.lastname: Kodet
authors.forename: S
authors.initials: S
authors.lastname: André
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Gabius
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018920:Coculture Techniques; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D007633:Keratins / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009368:Neoplasm Transplantation; D010641:Phenotype; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D015415:Biomarkers; D017931:DNA Primers; D005819:Genetic Markers; D007633:Keratins