Polymers containing isonitrile functional groups as supports for the covalent fixation of biologically active molecules.
1) Isonitrile derivatives of synthetic polyamides, polyesters and polyalcohols, of polysaccharides such as cellulose, cross-linked dextran and agarose, linear dextran and of cross-linked and linear polyacrylamide were prepared. 2) Proteins and low-molecular-weight ligands were bound covalently to these materials by four-component reactions involving the -NC function on the polymer, amino or carboxyl groups deriving from the ligand, aldehyde and the complementary fourth component (-COOH or - NH2) being added to the aqueous reaction medium. 3) Supports of modified surface properties could be prepared by grafting of water-soluble macromolecules containing -NC groups onto preformed polymeric structures. 4) The isonitrile functional groups on polymers could be transformed into other types of functional groups by simple one-step reactions.
Goldstein
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Department of Biochemistry, The George S. Wise Center for Life Sciences, Tel Aviv University, Tel Aviv, Israel.
D000438:Alcohols / Q000737:chemistry; D001671:Biochemistry / Q000379:methods*; D002482:Cellulose / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry; D008024:Ligands; D008956:Models, Chemical; D009570:Nitriles / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012685:Sepharose / Q000737:chemistry; D013499:Surface Properties; D014357:Trypsin / Q000737:chemistry; D014867:Water / Q000737:chemistry
D000438:Alcohols; D003432:Cross-Linking Reagents; D003911:Dextrans; D008024:Ligands; D009570:Nitriles; D009757:Nylons; D011091:Polyesters; D011108:Polymers; D014867:Water; D002482:Cellulose; D012685:Sepharose; D014357:Trypsin