title: Production of feruloyl esterases and xylanases by Talaromyces stipitatus and Humicola grisea var. thermoidea on industrial food processing by-products.
abstract: Feruloyl esterase (FAE) and xylanase activities were detected in culture supernatants from Humicola grisea var. thermoidea and Talaromyces stipitatus grown on brewers' spent grain (BSG) and wheat bran (WB), two agro-industrial by-products. Maximum activities were detected from cultures of H. grisea grown at 150 rpm, with 16.9 U/ml and 9.1 U/ml of xylanase activity on BSG and WB, respectively. Maximum FAE activity was 0.47 U/ml and 0.33 U/ml on BSG and WB, respectively. Analysis of residual cell wall material after microbial growth shows the preferential solubilisation of arabinoxylan and cellulose, two main polysaccharides present in BSG and WB. The production of low-cost cell-wall-deconstructing enzymes on agro-industrial by-products could lead to the production of low-cost enzymes for use in the valorisation of food processing wastes.
authors.affiliation: Sustainability of the Food Chain Exploitation Platform, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom.
authors.forename: G
authors.initials: G
authors.lastname: Mandalari
authors.forename: G
authors.initials: G
authors.lastname: Bisignano
authors.forename: R B
authors.initials: RB
authors.lastname: Lo Curto
authors.forename: K W
authors.initials: KW
authors.lastname: Waldron
authors.forename: C B
authors.initials: CB
authors.lastname: Faulds
mesh_terms: D001203:Ascomycota / Q000201:enzymology* / Q000254:growth & development; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D002473:Cell Wall / Q000378:metabolism; D002523:Edible Grain / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D005525:Food-Processing Industry; D007220:Industrial Waste; D032901:Talaromyces / Q000201:enzymology* / Q000254:growth & development
chemical_list: D007220:Industrial Waste; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D043364:Endo-1,4-beta Xylanases