title: Nuclear magnetic resonance structural studies of membrane proteins in micelles and bilayers.
abstract: Nuclear magnetic resonance (NMR) spectroscopy enables determination of membrane protein structures in lipid environments, such as micelles and bilayers. This chapter outlines the steps for membrane-protein structure determination using solution NMR with micelle samples, and solid-state NMR with oriented lipid-bilayer samples. The methods for protein expression and purification, sample preparation, and NMR experiments are described and illustrated with examples from gamma and CHIF, two membrane proteins that function as regulatory subunits of the Na+- and K+-ATPase.
authors.affiliation: The Burnham Institute, La Jolla, CA, USA.
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Gong
authors.forename: Carla M
authors.initials: CM
authors.lastname: Franzin
authors.forename: Khang
authors.initials: K
authors.lastname: Thai
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.forename: Francesca M
authors.initials: FM
authors.lastname: Marassi
mesh_terms: D000818:Animals; D015870:Gene Expression; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D008823:Micelles; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D000254:Sodium-Potassium-Exchanging ATPase / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins; D008823:Micelles; D011994:Recombinant Proteins; C422209:corticosteroid hormone-induced factor; D000254:Sodium-Potassium-Exchanging ATPase