Biocatalytic reductions: from lab curiosity to "first choice".
Enzyme-catalyzed reductions have been studied for decades and have been introduced in more than 10 industrial processes for production of various chiral alcohols, alpha-hydroxy acids and alpha-amino acids. The earlier hurdle of expensive cofactors was taken by the development of highly efficient cofactor regeneration methods. In addition, the accessible number of suitable dehydrogenases and therefore the versatility of this technology is constantly increasing and currently expanding beyond asymmetric production of alcohols and amino acids. Access to a large set of enzymes for highly selective C=C reductions and reductive amination of ketones for production of chiral secondary amines and the development of improved D-selective amino acid dehydrogenases will fuel the next wave of industrial bioreduction processes.
De Wildeman
Stefaan M A
SM
DSM Pharmaceuticals Products, Advanced Synthesis, Catalysis and Development, PO Box 18, 6160 MD Geleen, The Netherlands.
Sonke
Theo
T
Schoemaker
Hans E
HE
May
Oliver
O
D000438:Alcohols / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry; D002384:Catalysis; D003067:Coenzymes / Q000737:chemistry; D007651:Keto Acids / Q000737:chemistry; D007659:Ketones / Q000737:chemistry; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry*
D000438:Alcohols; D000596:Amino Acids; D003067:Coenzymes; D007651:Keto Acids; D007659:Ketones; D010088:Oxidoreductases