title: Chemiluminescent probe for the in vitro detection of protease activity.
abstract: A strategy involving the use of a self-immolative linker has been investigated for the chemiluminescent sensing of proteases. The reactive linker enabled the release of a 1,2-dioxetane light precursor. As a proof of principle, caspase-3, a key peptidase involved in apoptosis has been targeted. An in vitro assay has been carried out and proved the decomposition of the linker and the selectivity for caspase-3.
authors.affiliation: Equipe de Chimie Bio-Organique, Université de Rouen, INSA de Rouen, CNRS UMR 6014, COBRA, IRCOF, 1 rue Lucien Tesnière, Mont-Saint-Aignan Cedex, France
authors.forename: Jean-Alexandre
authors.initials: JA
authors.lastname: Richard
authors.forename: Ludovic
authors.initials: L
authors.lastname: Jean
authors.forename: Anthony
authors.initials: A
authors.lastname: Romieu
authors.forename: Marc
authors.initials: M
authors.lastname: Massonneau
authors.forename: Pauline
authors.initials: P
authors.lastname: Noack-Fraissignes
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Renard
mesh_terms: D003432:Cross-Linking Reagents / Q000737:chemistry; D010450:Endopeptidases / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D062385:Hydroxybenzoates / Q000737:chemistry; D015394:Molecular Structure
chemical_list: D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D062385:Hydroxybenzoates; D010450:Endopeptidases; C017616:phenolic acid