title: [Use of diafiltration in the lavage of cultures of Leptospira interrogans canicola canicola for the production of vaccine vax-SPIRAL].
abstract: It was proposed to substitute the present technology of the lavage operation for a tangential microfiltration system at the lavage stages of the culture of Leptospira interrogans canicola canicola. It was proved that this system may be applied, since no cellular affectation was observed and high pollutant elimination levels were obtained.
authors.affiliation: Instituto Finlay, Ciudad de La Habana, Cuba.
authors.forename: Kenia Fernández
authors.initials: KF
authors.lastname: Enrique
authors.forename: José Daniel Benitez
authors.initials: JD
authors.lastname: Zamora
authors.forename: Raúl Boué
authors.initials: RB
authors.lastname: Gutiérrez
authors.forename: Florinda Hernández
authors.initials: FH
authors.lastname: Torres
authors.forename: Janet Lazo
authors.initials: JL
authors.lastname: Airado
authors.forename: Damián Rojo
authors.initials: DR
authors.lastname: Hernández
mesh_terms: D001428:Bacterial Vaccines / Q000191:economics / Q000276:immunology / Q000592:standards; D001431:Bacteriological Techniques; D003462:Cuba; D005374:Filtration; D006801:Humans; D041341:Leptospira interrogans serovar canicola / Q000254:growth & development* / Q000276:immunology*; D007922:Leptospirosis / Q000517:prevention & control*; D013997:Time Factors
chemical_list: D001428:Bacterial Vaccines