An osmium-DNA interstrand complex: application to facile DNA methylation analysis.
Nucleic acids often acquire new functions by forming a variety of complexes with metal ions. Osmium, in an oxidized state, also reacts with C5-methylated pyrimidines. However, control of the sequence specificity of osmium complexation with DNA is still immature, and the value of the resulting complexes is unknown. We have designed a bipyridine-attached adenine derivative for sequence-specific osmium complexation. Sequence-specific osmium complexation was achieved by hybridization of a short DNA molecule containing this functional nucleotide to a target DNA sequence and resulted in the formation of a cross-linked structure. The interstrand cross-link clearly distinguished methylated cytosines from unmethylated cytosines and was used to quantify the degree of methylation at a specific cytosine in the genome.
Tanaka
Kazuo
K
Frontier Research System, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama 351-1098, Japan.
Tainaka
Kazuki
K
Umemoto
Tadashi
T
Nomura
Akiko
A
Okamoto
Akimitsu
A
D015082:2,2'-Dipyridyl / Q000737:chemistry; D000225:Adenine / Q000031:analogs & derivatives; D001483:Base Sequence; D003432:Cross-Linking Reagents / Q000737:chemistry; D003596:Cytosine / Q000031:analogs & derivatives; D004247:DNA / Q000737:chemistry*; D019175:DNA Methylation; D004591:Electrophoresis, Polyacrylamide Gel; D016678:Genome; D008745:Methylation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D009992:Osmium / Q000737:chemistry*; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D011743:Pyrimidines / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods*; D013696:Temperature
D003432:Cross-Linking Reagents; D009841:Oligonucleotides; D011743:Pyrimidines; D009992:Osmium; D015082:2,2'-Dipyridyl; D003596:Cytosine; D004247:DNA; D000225:Adenine