title: [Relationship of human leucocyte antigen G, E with intrahepatic cholestasis of pregnancy].
abstract: OBJECTIVE
  To investigate the relationship between human leucocyte antigen (HLA)-G, E and intrahepatic cholestasis of pregnancy (ICP).
  
  
  METHODS
  In situ hybridization and immunohistochemistry methods were adopted in this study to detect the expression of HLA-G and HLA-E mRNA and protein in the placentae of three groups, namely normal control group, ICP group with dexamethasone (DEX) treatment, and ICP group without DEX treatment.
  
  
  RESULTS
  No significant differences in respect to HLA-G, HLA-E mRNA expression on extrovillous cytotrophoblasts (EVCT ) of the placentae were found between the normal control group, the ICP group with DEX treatment and the ICP group without DEX treatment. However, the protein expression of HLA-G and HLA-E on the placental EVCT of ICP group without DEX treatment was significantly lower than that of the normal control group and the ICP group with DEX treatment.
  
  
  CONCLUSIONS
  The decreased EVCT expression of HLA-G, HLA-E protein may cause the disturbance of the maternal-fetal immune tolerance of ICP patient and play a role in the pathogenesis of ICP. DEX can upregulate the EVCT expression of HLA-G, HLA-E protein; this may be one of the underlying mechanisms for its use in the treatment of ICP.
authors.affiliation: Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China. pengbin-a111@163.com
authors.forename: Bing
authors.initials: B
authors.lastname: Peng
authors.forename: Shu-yun
authors.initials: SY
authors.lastname: Liu
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Wang
authors.forename: He
authors.initials: H
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Hu
mesh_terms: D016022:Case-Control Studies; D002780:Cholestasis, Intrahepatic / Q000188:drug therapy / Q000235:genetics* / Q000276:immunology; D003907:Dexamethasone; D005260:Female; D006680:HLA Antigens / Q000235:genetics* / Q000276:immunology; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology; D006801:Humans; D010920:Placenta / Q000276:immunology / Q000378:metabolism; D011247:Pregnancy; D011248:Pregnancy Complications / Q000235:genetics* / Q000276:immunology; D000097625:HLA-E Antigens
chemical_list: D003907:Dexamethasone; D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D000097625:HLA-E Antigens