Application of micro-solid-phase extraction for the determination of persistent organic pollutants in tissue samples.
A novel, simple and efficient method for determining persistent organic pollutants (POPs) in tissue samples has been developed. This technique involves the use of simultaneous microwave-assisted digestion (MAD) and micro-solid-phase extraction (micro-SPE), in which the sorbent is held within a propylene membrane envelope, with gas chromatographic-mass spectrometric (GC-MS) analysis. The POPs studied included eleven organochlorine pesticides and five polychlorinated biphenyl congeners. Optimization of the MAD-micro-SPE parameters was performed. The relative standard deviations (RSDs) of the method ranged from 0.14 to 12.7%. Correlation coefficients up to 0.9999 were obtained across a concentration range of 1.25-50 ng g(-1). The method detection limits for POPs ranged from 0.002 to 0.009 ng g(-1). A preliminary study applying the MAD-micro-SPE procedure to human ovarian cancer tissue showed that it was capable of detecting the presence of a wide spectrum of different POPs in benign and malignant tumors.
Basheer
Chanbasha
C
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
Narasimhan
Kothandaraman
K
Yin
Meihui
M
Zhao
Changqing
C
Choolani
Mahesh
M
Lee
Hian Kee
HK
D002138:Calibration; D004785:Environmental Pollutants / Q000032:analysis*; D005260:Female; D006801:Humans; D008872:Microwaves; D009930:Organic Chemicals / Q000032:analysis*; D010051:Ovarian Neoplasms / Q000737:chemistry*; D010053:Ovary / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D012997:Solvents; D013696:Temperature; D013997:Time Factors
D004785:Environmental Pollutants; D009930:Organic Chemicals; D012997:Solvents