title: [A technique for investigating the component of cantharidin blisters by flow cytometry].
abstract: OBJECTIVE
  Cantharidimide cause blister. The effect of blister on immunoregulation was investigated.
  
  
  METHODS
  Cantharidimide was placed on the skin, 48h later, the blister was analyzed by flow cytometry.
  
  
  RESULTS
  The blister contained 1 x 10(6) - 1 x 10(7) cells per ml, most of which were neutrophils, macrophages, dendritic cells (DC), and IL-12 secreted by Thl cells.
  
  
  CONCLUSIONS
  There are high concent of DC in the blister, which is differential and induce the secretion of Th1, the activation of T cell. The blister modulate the biological response of patients and is helpful for treatment with infective disease.
authors.affiliation: Department of Infectious Diseases, Communicable Diseases Center of PLA, The 458th Hospital of PLA, Guangzhou 510602, China. kyzouxj@163.com
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Zou
authors.forename: Hui-ping
authors.initials: HP
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Cheng
authors.forename: Li
authors.initials: L
authors.lastname: Shi
authors.forename: Ping
authors.initials: P
authors.lastname: Zu
authors.forename: Yi-jun
authors.initials: YJ
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D001768:Blister / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D001826:Body Fluids / Q000166:cytology / Q000276:immunology; D002193:Cantharidin / Q000506:poisoning*; D001517:Coleoptera / Q000737:chemistry; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D018664:Interleukin-12 / Q000096:biosynthesis; D007509:Irritants / Q000506:poisoning; D008297:Male; D008419:Materia Medica / Q000737:chemistry*; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
chemical_list: D015703:Antigens, CD; D007509:Irritants; D008419:Materia Medica; D018664:Interleukin-12; D002193:Cantharidin