title: Shuffling of pig interleukin-2 gene and its enhancing of immunity in mice to Pasteurella multocida vaccine.
abstract: In order to explore the safe and effective adjuvant for promotion of immunity of animals against infection, the experiment was carried out to shuffle Tibet pig IL-2 cDNA with other IL-2 cDNA from human, yak and mouse, and the effect of shuffled IL-2 (IL-2S) gene in vivo was investigated on immunity of mice to Pasteurella multocida. The IL-2S protein was found to remarkably promote the proliferation of pig lymphoblasts than the native pig IL-2 protein. Then the IL-2S gene was cloned into VR1020 eukaryotic plasmid (VRIL2S) and enwrapped with chitosan nanoparticles (CNP-VRIL2S). Twenty-one day old female Kunming mice were muscularly inoculated respectively with the CNP-VRIL2, CNP-VRIL2S and CNP-VR1020 along with Pasteurella multocida vaccine, and orally challenged with virulent Pasteurella multocida on 28 days post-vaccination. The blood was weekly collected to detect the change of IgG, IgA, IgM, specific antibody, IL-2, IL-4 and IL-6 by ELISA. The immunoglobulins, specific antibody and interleukins significantly increased in CNP-VRIL2S group compared with the control mice after vaccination and challenge, and 9 of 10 immunized mice survived challenge, while the all control mice manifested severe symptoms and lesions, and finally died of infection. These indicated that VRIL2S entrapped with CNP is a novel safe and effective adjuvant to boost the specific immunity and resistance of animal against infectious pathogen, which could facilitate the development of highly promising powerful adjuvant.
authors.affiliation: Key Laboratory for Bio-Resource and Eco-Environment of Ministry of Education, Bioengineering Research Center for Animal Disease Prevention and Control, Life college of Sichuan University, 610064, Chengdu, Sichuan, People's Republic China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Xie
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Jianlin
authors.initials: J
authors.lastname: Chen
authors.forename: Hua-Bing
authors.initials: HB
authors.lastname: Zhang
authors.forename: Ying-Yu
authors.initials: YY
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.forename: Zhong-Zhong
authors.initials: ZZ
authors.lastname: Zhao
authors.forename: Chi
authors.initials: C
authors.lastname: Cheng
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Hong-Ning
authors.initials: HN
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Gao
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D048271:Chitosan / Q000737:chemistry*; D007376:Interleukin-2 / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry; D010326:Pasteurella Infections / Q000276:immunology* / Q000517:prevention & control / Q000662:veterinary; D016979:Pasteurella multocida / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000276:immunology; D013552:Swine / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D007376:Interleukin-2; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D048271:Chitosan