c-Abl is required for the signaling transduction induced by L-selectin ligation.
Lymphocyte recruitment onto inflamed tissues requires cells tethering to and rolling on vascular surfaces under flow. L-selectin is constitutively expressed on leukocytes to mediate the leukocytes' initial capture and subsequent rolling along the vessel. Apart from its adhesive function, engagement of L-selectin also results in cell activation, which is related to the completed signaling transduction. Here we show that ligation of L-selectin with its mAb increases c-Abl kinase activity, and that the activated c-Abl kinase can be recruited to and phosphorylate the cytoplasmic domain of L-selectin. In addition, the activated c-Abl kinase can regulate Zap70 kinase by increasing the phosphorylation of the Y319 site of Zap70 kinase and connect with Zap70 kinase through its SH2 domain. These results indicate that c-Abl kinase plays an important role in accepting and transferring the upstream activation events induced by L-selectin ligation.
Chen
Cuixia
C
Institute of Genetics and Cytology, Northeast Normal University, Changchun, PR China.
Shang
Xin
X
Xu
Ting
T
Cui
Lingling
L
Luo
Jixian
J
Ba
Xueqing
X
Hao
Shui
S
Zeng
Xianlu
X
D015153:Blotting, Western; D006801:Humans; D047468:Immunoprecipitation; D019169:Jurkat Cells; D019041:L-Selectin / Q000378:metabolism*; D036904:Leukocyte Rolling / Q000502:physiology; D008213:Lymphocyte Activation / Q000276:immunology; D016315:Proto-Oncogene Proteins c-abl / Q000378:metabolism*; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000276:immunology*; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D014158:Transcription, Genetic; D051746:ZAP-70 Protein-Tyrosine Kinase
D019041:L-Selectin; D016186:Receptor, Macrophage Colony-Stimulating Factor; D016315:Proto-Oncogene Proteins c-abl; D051746:ZAP-70 Protein-Tyrosine Kinase; C497261:ZAP70 protein, human