title: In vitro studies on liposomal amphotericin B obtained by supercritical carbon dioxide-mediated process.
abstract: Nanotechnology in drug delivery is a rapidly expanding field. Nanosized liposomal preparations are already in use for efficient drug delivery with better therapeutic indices. Existing methods of liposome preparation are limited by problems of scale-up, difficulty in controlling size, and intercalation efficiency. Here we prepare amphotericin B-intercalated liposomes by a novel process where amphotericin B and purified phosphatidyl choline are solubilized in suitable solvent and precipitated in supercritical fluid carbon dioxide (known as a gas antisolvent technique), to obtain microsized particles that are subsequently introduced into a buffer solution. The morphology of liposomes was characterized through a phase-contrast microscope, and the particle size distribution studied by laser technique showed nanosize with a narrow range of size distribution (between 0.5 and 15 microm) and a higher intercalation efficiency. In vitro studies conducted using Aspergillus fumigatus (MTCC 870) strain proved to be efficient in the retardation of the growth of the organism.
authors.affiliation: Food Engineering Centre, Central Food Technological Research Institute, Mysore, India. udaya@cftri.res.in
authors.forename: Udaya Sankar
authors.initials: US
authors.lastname: Kadimi
authors.forename: Deepan Raja
authors.initials: DR
authors.lastname: Balasubramanian
authors.forename: Usha Rani
authors.initials: UR
authors.lastname: Ganni
authors.forename: Manohar
authors.initials: M
authors.lastname: Balaraman
authors.forename: Venkateswaran
authors.initials: V
authors.lastname: Govindarajulu
mesh_terms: D000666:Amphotericin B / Q000008:administration & dosage / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D002245:Carbon Dioxide / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D004058:Diffusion; D004337:Drug Carriers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D008081:Liposomes; D002245:Carbon Dioxide; D000666:Amphotericin B