A rapid CE-potential gradient detection method for determination of quinolones.
A rapid CE coupled with potential gradient detection (PGD) for the separation and detection of four quinolones, namely, enoxacin, ofloxacin (OFL), fleroxacin, and pazufloxacin, was described. Separation was performed in a fused-silica capillary (75 microm x 8.5 cm) using a buffer of 30 mM Tris and 4 mM phosphoric acid at pH 8.9. Under the separation voltage of 3 kV, the quinolones were separated within 2.8 min with good linearity (r(2) >or= 0.985). The method was successfully applied in determining OFL in a pharmaceutical formulation. Also, a liquid-liquid extraction (LLE) method was developed and coupled to CE-PGD in determining quinolones that spiked in milk samples. With dichloromethane and hexane for enrichment and purification, the LLE recoveries of the four quinolones were in the range of 77-106%. The detection limits of the quinolones with LLE-CE-PGD were from 23 to 65 ng/mL. The proposed CE-PGD method was validated with an HPLC method, and the results indicated consistency between the two methods.
Fan
Yongxia
Y
College of Chemistry, Beijing Normal University, Beijing, PR China.
Gan
Xiao
X
Li
Shuo
S
Qin
Weidong
W
D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D015365:Enoxacin / Q000032:analysis; D016576:Fleroxacin / Q000032:analysis; D024841:Fluoroquinolones / Q000032:analysis; D008722:Methods; D008892:Milk / Q000737:chemistry; D015242:Ofloxacin / Q000032:analysis; D010078:Oxazines / Q000032:analysis; D015363:Quinolones / Q000032:analysis*
D024841:Fluoroquinolones; D010078:Oxazines; D015363:Quinolones; D015365:Enoxacin; C078052:pazufloxacin; D015242:Ofloxacin; D016576:Fleroxacin