[Start-up, formation and microbial community analysis of aerobic granules in SABR for treatment of organic wastewater containing aniline and chloroanilines].
The granulation of aerobic sludges for high-rate biodegradation of organic wastewater containing aniline and chloroanilines was investigated in a laboratory-scale sequencing airlift bioreactor ( SABR). Aerobic granules were observed in 15 days after start-up in SABR. After subsequent 83 days, SABR was operated sequentially in superficial air velocity of 2.4cm/s, COD loadings of 1.0-3.6kg/(m3 x d) and (chloro-) anilines loadings increased stepwise to 1kg/(m3 x d), a steady-state performance of aerobic granular SABR was achieved at last, as evidenced by high and stable COD and (chloro-) anilines removal efficiencies of above 90% and 99.9%, respectively. Mature granules with median size of 0.45-2.5mm, minimal settling velocity of 62. 1m/h, and SVI of 56mL/g were developed. Aerobic granular sludge displayed noteworthy SOUR, specific (chloro-) anilines degradation rates, PN content and PN/PS ratio in EPS extracts as 154mgDO/(gVSS x h), 0.18g/(gVSS x d), 28.0 +/- 1.9mg/gVSS and 6.5mg/mg respectively, indicating that they had high activity and ability to withstand high (chloro-) anilines loadings. Phylogenetic analysis of (chloro-) anilines-degrading aerobic granules indicated that beta-, gamma-Proteobacteria and Flavobacteria were dominant classes and the predominance bacteria were closely related to Pseudomonas sp. and Flavo-bacterium sp. Compared to chloroanilines-degrading aerobic granules, the population diversity was higher in the aniline and chloroaniline-degrading aerobic granules.
Zhu
Liang
L
Department of Environmental Engineering, Zhejiang University, Hangzhou 310029, China. felix79cn@zju.edu.cn
Xu
Xiang-yang
XY
Cao
Dan-feng
DF
Luo
Wei-guo
WG
Yang
Yan-ni
YN
D000332:Aerobiosis; D000814:Aniline Compounds / Q000737:chemistry / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D010802:Phylogeny; D012722:Sewage / Q000032:analysis* / Q000382:microbiology
D000814:Aniline Compounds; D012722:Sewage; C023650:aniline