Expression analysis and tissue distribution of two 14-3-3 proteins in silkworm (Bombyx mori).
14-3-3 proteins, which have been identified in a wide variety of eukaryotes, are highly conserved acidic proteins. In this study, we identified two genes in silkworm that encode 14-3-3 proteins (Bm14-3-3zeta and Bm14-3-3epsilon). Category of two 14-3-3 proteins was identified according to phylogenetic analysis. Bm14-3-3zeta shared 90% identity with that in Drosophila, while Bm14-3-3epsilon shared 86% identity with that in Drosophila. According to Western blot and real time PCR analysis, the Bm14-3-3zeta expression levels are higher than Bm14-3-3epsilon in seven tissues and in four silkworm developmental stages examined. Bm14-3-3zeta was expressed during every stage of silkworm and in every tissue of the fifth instar larvae that was examined, but Bm14-3-3epsilon expression was not detected in eggs or heads of the fifth instar larvae. Both 14-3-3 proteins were highly expressed in silk glands. These results suggest that Bm14-3-3zeta expression is universal and continuous, while Bm14-3-3epsilon expression is tissue and stage-specific. Based on tissue expression patterns and the known functions of 14-3-3 proteins, it may be that both 14-3-3 proteins are involved in the regulation of gene expression in silkworm silk glands.
Kong
Lingyin
L
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China.
Lv
Zhengbing
Z
Chen
Jian
J
Nie
Zuoming
Z
Wang
Dan
D
Shen
Hongdan
H
Wang
Xuedong
X
Wu
Xiangfu
X
Zhang
Yaozhou
Y
D048948:14-3-3 Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D048948:14-3-3 Proteins; D017931:DNA Primers; D018076:DNA, Complementary