title: Reconstitution of glyphosate resistance from a split 5-enolpyruvyl shikimate-3-phosphate synthase gene in Escherichia coli and transgenic tobacco.
abstract: A highly N-phosphonomethylglycine (glyphosate)-resistant Pseudomonas fluorescens G2 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) was mapped to identify potential split sites using a transposon-based linker-scanning procedure. Intein-mediated protein complementation was used to reconstitute glyphosate resistance from the genetically divided G2 EPSPS gene in Escherichia coli strain ER2799 and transgenic tobacco.
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agriculture Sciences, Beijing 100081, China. linmin57@vip.163.com.
authors.forename: Bao-Qing
authors.initials: BQ
authors.lastname: Dun
authors.forename: Xu-Jing
authors.initials: XJ
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Zhong-Lin
authors.initials: ZL
authors.lastname: Zhao
authors.forename: Song-Na
authors.initials: SN
authors.lastname: Hou
authors.forename: Bao-Ming
authors.initials: BM
authors.lastname: Zhang
authors.forename: Gui-Ying
authors.initials: GY
authors.lastname: Li
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Evans
authors.forename: Ming-Qun
authors.initials: MQ
authors.lastname: Xu
authors.forename: Min
authors.initials: M
authors.lastname: Lin
mesh_terms: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase / Q000235:genetics* / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000378:metabolism; D006540:Herbicides / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000201:enzymology / Q000235:genetics; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D000097797:Glyphosate
chemical_list: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D005998:Glycine; D006540:Herbicides; D000097797:Glyphosate