title: A simple and economical method for the production of 13C,18O-labeled Fmoc-amino acids with high levels of enrichment: applications to isotope-edited IR studies of proteins.
abstract: Isotope-edited IR of proteins has generated considerable interest. Double labeling with 13C and 18O with high levels of isotopic enrichment is required for residue-specific resolution. Current methods for the preparation of doubly labeled amino acids give modest 18O enrichment, limiting the utility of the approach. We report a simple and economical method for preparing 13C,18O-doubly labeled N-(9-fluorenylmethoxycarbonyl)amino acids with high levels of enrichment for residues that do not require acid-labile side-chain protecting groups.
authors.affiliation: Department of Chemistry, State University of New York at Stony Brook, Stony Brook, New York 11794-3400, USA. james.mareck@stonybrook.edu
authors.forename: James
authors.initials: J
authors.lastname: Marecek
authors.forename: BenBen
authors.initials: B
authors.lastname: Song
authors.forename: Scott
authors.initials: S
authors.lastname: Brewer
authors.forename: Jenifer
authors.initials: J
authors.lastname: Belyea
authors.forename: R Brian
authors.initials: RB
authors.lastname: Dyer
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Raleigh
mesh_terms: D000596:Amino Acids / Q000138:chemical synthesis* / Q000737:chemistry*; D002247:Carbon Isotopes; D005449:Fluorenes / Q000138:chemical synthesis* / Q000737:chemistry*; D010103:Oxygen Isotopes; D011506:Proteins / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013055:Spectrophotometry, Infrared / Q000379:methods
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D010103:Oxygen Isotopes; D011506:Proteins