Lepidopteran insect cell line isolation from insect tissue.
This chapter describes procedures for initiating new cell lines from lepidopteran larval tissues. The internal morphology is described along with methods for treating excised tissues and the primary cultures. Advice on culture medium and the tissues that will provide the best chance for new cell lines is discussed.
Lynn
Dwight E
DE
USDA/ARS Insect Biocontrol Laboratory, Beltsville, MD, USA.
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D007814:Larva / Q000033:anatomy & histology / Q000166:cytology; D007915:Lepidoptera / Q000033:anatomy & histology / Q000166:cytology*