title: O-glycan regulation of apoptosis and proliferation in colorectal cancer cell lines.
abstract: Cell growth pathways are mediated through protein-glycan interactions including O-glycosylation. Investigation of these growth pathways can be carried out using appropriate inhibitors to identify stage-specific events. We have adopted this approach to study a group of benzyl-O-N-acetyl-D-galactosamine analogues in human colorectal cancer cell lines. Exposure to O-glycan inhibitors resulted in the induction of apoptosis, a block in proliferation, accumulation of intracellular aryl-glycans and changes in related genes as detected by gene array. Colorectal cancer cell lines susceptible to the inhibitors showed growth arrest with all compounds. However, a differential action of each inhibitor was detected in the pattern of genes affected and in the structure of aryl-glycans formed.
authors.affiliation: Mucin Research Group, Clinical Science@South Bristol, Bristol BS2 8HW, U.K.
authors.forename: G
authors.initials: G
authors.lastname: Patsos
authors.forename: C
authors.initials: C
authors.lastname: Robbe-Masselot
authors.forename: A
authors.initials: A
authors.lastname: Klein
authors.forename: V
authors.initials: V
authors.lastname: Hebbe-Viton
authors.forename: R San
authors.initials: RS
authors.lastname: Martin
authors.forename: D
authors.initials: D
authors.lastname: Masselot
authors.forename: M
authors.initials: M
authors.lastname: Graessmann
authors.forename: C
authors.initials: C
authors.lastname: Paraskeva
authors.forename: T
authors.initials: T
authors.lastname: Gallagher
authors.forename: A
authors.initials: A
authors.lastname: Corfield
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D006031:Glycosylation; D006801:Humans; D011134:Polysaccharides / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D011134:Polysaccharides