Increased expression of intracystic matrix metalloproteinases in brain tumors: relationship to the pathogenesis of brain tumor-associated cysts and peritumoral edema.
Although several types of brain tumors are commonly associated with cyst formation, the pathogenesis of tumor-associated cysts (TAC) is unknown. We investigated the matrix metalloproteinase (MMP) expression of cyst fluids to elucidate the pathogenesis of TAC in brain tumors. We also examined the relationship between the severity of peritumoral edema and the expression of intracystic MMP. We collected 40 cyst fluid samples from 34 patients with TAC and studied the expression of MMP-2 and -9 in the cyst fluid using gelatin zymography. Radiological studies were used to estimate the severity of the peritumoral edema and to determine the presence of TAC. Although gelatin zymography of the cyst fluid showed high levels of MMPs, there was no correlation between the expression of MMPs in the cyst fluid and that in the tumor tissue. The level of MMP expression in the cyst fluid did not reflect the pathologic grade of the individual tumors. However, the total and activated MMP-9 levels were significantly associated with the severity of the peritumoral edema (p<0.05). These results suggest that MMPs may be partly involved in the pathogenesis of TAC and peritumoral edema in brain tumors.
Jung
Shin
S
Department of Neurosurgery, Chonnam National University, Hwasun Hospital and Medical School, Gwangju, South Korea. sjung@chonnam.ac.kr
Moon
Kyung-Sub
KS
Kim
Sung-Taek
ST
Ryu
Hyang-Hwa
HH
Lee
Young-Hwa
YH
Jeong
Young-Il
YI
Jung
Tae-Young
TY
Kim
In-Young
IY
Kim
Kyung-Keun
KK
Kang
Sam-Suk
SS
D001929:Brain Edema / Q000201:enzymology* / Q000209:etiology / Q000473:pathology*; D001932:Brain Neoplasms / Q000150:complications / Q000201:enzymology* / Q000473:pathology*; D020863:Central Nervous System Cysts / Q000201:enzymology* / Q000209:etiology / Q000473:pathology*; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000134:cerebrospinal fluid; D008279:Magnetic Resonance Imaging; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000134:cerebrospinal fluid; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000134:cerebrospinal fluid; D020782:Matrix Metalloproteinases / Q000096:biosynthesis* / Q000134:cerebrospinal fluid
D007527:Isoenzymes; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9