[Expressions of c-mpl proteins on CD34+ bone marrow cells and platelets of the patients with polycythemia vera].
The objective of this study was to investigate the expressions of TPO receptor (c-mpl) proteins on CD34 positive bone marrow cells (CD34+ BMCs), platelets and the expression of c-mpl mRNA in bone marrow cells of the patients with polycythemia vera (PV). The expressions of c-mpl proteins on CD34+ bone marrow hematopoietic cells of 13 PV patients and 15 normal controls were assessed by bicolor flow cytometry and the expressions of c-mpl proteins on platelets of 15 PV patients and 15 normal controls were assessed by monocolor flow cytometry, and the expressions of c-mpl mRNA in bone marrow hematopoietic cells (BMHCs) were assessed by RT-PCR. The results showed that no difference was found between the expression of c-mpl proteins on CD34+ BMHCs of PV patients (0.99% +/- 0.14%) and that of normal controls (0.92% +/- 0.12%) (p > 0.05). There was no difference too between the expression of c-mpl protein on platelets in PV patients (20.33% +/- 4.84%) and that in normal controls (23.50% +/- 3.64%) (p > 0.05). No difference between the expression of c-mpl mRNA in BMHCs of PV patients and that in normal controls was seen. In conclusion, the expressions of c-mpl proteins on CD34+ BMHCs, platelets and c-mpl mRNA in BMHCs of PV patients were not obviously abnormal.
Bai
Jie
J
Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences Peking Union Medical College, 300020 Tianjin, China.
Shao
Zong-Hong
ZH
Shi
Jun
J
Jia
Hai-Rong
HR
Sun
Juan
J
D018952:Antigens, CD34 / Q000032:analysis; D001792:Blood Platelets / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology; D006412:Hematopoietic Stem Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D011087:Polycythemia Vera / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D053628:Receptors, Thrombopoietin / Q000378:metabolism*
D018952:Antigens, CD34; D012333:RNA, Messenger; D053628:Receptors, Thrombopoietin; C082218:MPL protein, human