title: Evaluation of the changes of immune cells during lipopolysaccharide-induced mastitis in rats.
abstract: The aim of this study was to evaluate in rats, changes in peripheral blood immune cells and mammary tissue after an intramammary infusion of lipopolysaccharide (LPS). The results of the study showed that infusion of LPS induced a rapid migration of neutrophils (PMNs) from the blood to mammary alveoli, increased the activity of myeloperoxidase (MPO) and the concentration of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and interleukin-6 (IL-6) in mammary tissues, decreased the activity of myeloperoxidase in serum and reduced the CD4+/CD8+ ratio. This is the first report of changes in peripheral blood immune cells and mammary tissue in rat mastitis.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Miao
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Zhu
authors.forename: Bei-Bei
authors.initials: BB
authors.lastname: Gu
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Wang
authors.forename: Si-Xiang
authors.initials: SX
authors.lastname: Zou
authors.forename: Yue-E
authors.initials: YE
authors.lastname: Deng
mesh_terms: D000818:Animals; D016516:CD4-CD8 Ratio; D005260:Female; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity*; D008321:Mammary Glands, Animal / Q000378:metabolism* / Q000473:pathology; D008413:Mastitis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D020556:Neutrophil Infiltration / Q000187:drug effects*; D009504:Neutrophils / Q000378:metabolism* / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D009195:Peroxidase