Identification and characterization of thymosin beta-4 in chicken macrophages using whole cell MALDI-TOF.
The aim of the study was to determine chicken monocyte- and granulocyte-associated peptides and proteins using "whole cell" matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and to characterize the peptides based on their abundance. The mass spectra showed a prominent peak at m/z 4963 in monocytes/macrophages but not in the granulocytes. Subsequent purification and characterization of the m/z 4963 peptide from an avian macrophage cell line HTC, revealed it to be thymosin beta-4 (Tbeta-4), an actin-modulating peptide. HTC cells when treated with bacterial lipopolysaccharide and peptidoglycan to determine the modulation of Tbeta-4 gene expression or its secretion, showed no changes.
Kannan
Lakshmi
L
Agricultural Research Service, USDA, Poultry Science Center, University of Arkansas, Fayetteville, AR 72701, USA.
Rath
Narayan C
NC
Liyanage
Rohana
R
Lay
Jackson O
JO
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D005786:Gene Expression Regulation; D006098:Granulocytes / Q000737:chemistry / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000502:physiology; D008264:Macrophages / Q000737:chemistry* / Q000502:physiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013947:Thymosin / Q000032:analysis* / Q000235:genetics*; D000099201:Thymosin beta(4)
D013947:Thymosin; D000099201:Thymosin beta(4)