title: Role of bacterial OmpA and host cytoskeleton in the invasion of human intestinal epithelial cells by Enterobacter sakazakii.
abstract: Enterobacter sakazakii is an emerging pathogen in neonates and infants. Interactions of E. sakazakii with intestinal epithelium could be vital in the pathogenesis of enteric infections and in its systemic dissemination. The present study investigated the interaction of E. sakazakii with human intestinal epithelial (INT407) cells and the role of bacterial outer membrane protein A (OmpA) and host cytoskeleton in these interactions. E. sakazakii invaded INT407 cells with moderate efficiency. An ompA mutant of E. sakazakii was significantly attenuated in its invasiveness, and complementation restored the invasive phenotype significantly. Drugs acting on host cell microfilaments (MF) and microtubules (MT) significantly inhibited bacterial invasion. Localization of both microfilaments (MF) and microtubules (MT) was observed in INT407 cells following E. sakazakii infection. The results suggest that E. sakazakii invasion of INT407 cells involves participation of both MF and MT and bacterial OmpA plays a critical role in invasion.
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Mohan Nair
authors.forename: Kumar
authors.initials: K
authors.lastname: Venkitanarayanan
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism*; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D044083:Cronobacter sakazakii / Q000378:metabolism / Q000472:pathogenicity*; D015638:Cytochalasin D / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology*; D005353:Fibronectins / Q000378:metabolism; D005838:Genotype; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects / Q000196:embryology / Q000378:metabolism / Q000382:microbiology*; D018613:Microscopy, Confocal; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D009154:Mutation; D015739:Nocodazole / Q000494:pharmacology; D010641:Phenotype; D050257:Tubulin Modulators / Q000494:pharmacology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D005353:Fibronectins; D050257:Tubulin Modulators; C078167:OMPA outer membrane proteins; D015638:Cytochalasin D; D015739:Nocodazole