Screening of a microvascular endothelial cDNA library identifies rabaptin 5 as a novel autoantigen in Alzheimer's disease.
The pathogenesis of Alzheimer's disease (AD) includes the participation of the immune system. To identify antigenic targets in AD, we screened a human microvascular endothelial cell cDNA library with sera from patients with AD, and we identified rabaptin 5 (RABPT5). We detected serum IgG specific to RABPT5 in 65% of patients with AD and in 35% of patients with systemic lupus erythematosus, but in no healthy controls. Our results demonstrated a massive redistribution of this protein in the cytoplasm of endothelial and neuronal cells in apoptosis. In conclusion, we identified RABPT5 as a novel autoantigen in AD.
Delunardo
Federica
F
Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Rome, Italy.
Margutti
Paola
P
Pontecorvo
Simona
S
Colasanti
Tania
T
Conti
Fabrizio
F
Riganò
Rachele
R
Profumo
Elisabetta
E
Siracusano
Alessandra
A
Capozzi
Antonella
A
Prencipe
Massimiliano
M
Sorice
Maurizio
M
Francia
Ada
A
Ortona
Elena
E
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000276:immunology*; D000595:Amino Acid Sequence; D001324:Autoantigens / Q000378:metabolism*; D016009:Chi-Square Distribution; D042783:Endothelial Cells / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015723:Gene Library; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D033921:Vesicular Transport Proteins / Q000276:immunology*
D001324:Autoantigens; C097227:RABEP1 protein, human; D033921:Vesicular Transport Proteins