title: [Effects of EPA, DHA on the secretion of NO, expression of iNOS mRNA and DNA-binding activity of NFkappaB in human monocyte].
abstract: OBJECTIVE
  To study the effect of n-3 polyunsaturated fatty acids (EPA, DHA) on the production of NO, expression of iNOS mRNA and DNA-binding activity of NFkappaB in human monocyte.
  
  
  METHODS
  RT-PCR and enzyme reduction method were used to measure the expression of iNOS mRNA and production of NO respectively. The DNA-binding activity of NFkappaB was assessed by EMSA.
  
  
  RESULTS
  EPA, DHA can decrease the production of NO, expression of iNOS mRNA and DNA-binding activity of NFkappaB between the dosage of 10microg/ml and 20microg/ml in human peripheral blood monocyte in vitro.
  
  
  CONCLUSIONS
  EPA, DHA have inhibitory effects on NO production, iNOS mRNA expression and DNA-binding activity of NFkappaB in monocyte. It indicates EPA, DHA suppress the production of inflammatory mediators via the NFkappaB signal transduction pathways in human monocyte, accordingly suppress inflammatory responses.
authors.affiliation: Faculty of Medical Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Xia
authors.forename: Xiang
authors.initials: X
authors.lastname: Feng
authors.forename: Yuming
authors.initials: Y
authors.lastname: Chen
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Su
mesh_terms: D004247:DNA / Q000378:metabolism; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D006801:Humans; D009000:Monocytes / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D016328:NF-kappa B; D012333:RNA, Messenger; D004281:Docosahexaenoic Acids; D009569:Nitric Oxide; D004247:DNA; D015118:Eicosapentaenoic Acid; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II