An impedimetric sensor for monitoring the growth of Staphylococcus epidermidis.
There is a need for accurate, reliable methods of detecting bacteria for a range of applications. One organism that is commonly found in urinary catheter infections is Staphylococcus epidermidis. Current methods to determine the presence of an infection require the removal of catheters. An alternative approach may be the use of in vivo sensing for bacterial/biofilm detection. This work investigates electrical impedance spectroscopy to detect the growth of Staphylococcus epidermidis RP62A on gold electrodes fabricated on a flexible substrate. Impedance spectra measured during biofilm formation on the electrode surface showed an increase in charge transfer resistance (RCT) with time.
Oliver
Louise M
LM
Northern Ireland BioEngineering Centre, University of Ulster, N. Ireland, UK. l.oliver@ulster.ac.uk
Dunlop
P S M
PS
Byrne
J A
JA
Blair
I S
IS
Boyle
M
M
McGuigan
K G
KG
McAdams
E T
ET
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D049109:Cell Proliferation; D015169:Colony Count, Microbial / Q000295:instrumentation / Q000379:methods*; D017097:Electric Impedance; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*; D013212:Staphylococcus epidermidis / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology*