title: Interactions between loop 5 and beta-strand beta6' are involved in alpha7 nicotinic acetylcholine receptors channel gating.
abstract: Binding of agonists to nicotinic acetylcholine receptors (nAChR) is coupled to channel opening through local rearrangements of different domains of the protein. Recent structural data suggest that two of these regions could be the loop 5 (L5) and the beta-strand beta6', both forming the inner part of the N-terminal domain. Amino acids in these domains were mutated in alpha7 nAChRs, and expression levels and functional responses of mutant receptors were measured. Mutations located at the putative apex of L5, Asp97 and Glu98, and also at Phe100, gave receptors with smaller currents, showing qualitative differences with respect to muscle nAChRs. In contrast, mutations in the beta-strand beta6' (at Phe124 and Lys125) showed increased functional responses. Mutations affected equally the responses to acetylcholine and dimethylphenylpiperazinium, except in Phe100 where the latter was sevenfold less effective than in wild-type. Currents in mutants decayed with almost the same kinetics, ruling out large effects on desensitization. Analysis of double mutants demonstrated a functional coupling among the three electrically charged amino acids Asp97, Glu98, and Lys125, and also between Phe100 and Phe124. The results are compatible with the involvement of functional interactions between L5 and beta-strand beta6' during nAChR activation.
authors.affiliation: Instituto de Neurociencias de Alicante, Universidad Miguel Hernández-CSIC, Sant Joan d'Alacant, Alicante, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Criado
authors.forename: José
authors.initials: J
authors.lastname: Mulet
authors.forename: Mar
authors.initials: M
authors.lastname: Castillo
authors.forename: Marcos
authors.initials: M
authors.lastname: Aldea
authors.forename: Salvador
authors.initials: S
authors.lastname: Sala
authors.forename: Francisco
authors.initials: F
authors.lastname: Sala
mesh_terms: D000409:Alanine / Q000235:genetics; D019943:Amino Acid Substitution; D000818:Animals; D002038:Bungarotoxins / Q000493:pharmacokinetics; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects / Q000235:genetics; D009154:Mutation / Q000502:physiology; D018722:Nicotinic Agonists / Q000493:pharmacokinetics / Q000494:pharmacology; D009865:Oocytes; D011485:Protein Binding / Q000187:drug effects; D017434:Protein Structure, Tertiary / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011978:Receptors, Nicotinic / Q000737:chemistry* / Q000502:physiology*; D064569:alpha7 Nicotinic Acetylcholine Receptor
chemical_list: D002038:Bungarotoxins; D018722:Nicotinic Agonists; D012333:RNA, Messenger; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor; D000409:Alanine