title: Environmental dynamics of Bacillus amyloliquefaciens CCMI 1051 antifungal activity under different nitrogen patterns.
abstract: OBJECTIVE
  The aim of this study was to evaluate the influence of environmental conditions on the antifungal activity of the Bacillus sp. CCMI 1053 cultures.
  
  
  RESULTS
  The electrospray ionization mass spectra (ESI-MS) analysis was used to detect the active peptides produced by Bacillus amyloliquefaciens CCMI 1051 cultures in a glucose-containing medium to which four different nitrogen sources were added. The cultures produced different patterns of Bacillus sporulation and distinct antifungal activity of the cell-free culture broths.
  
  
  CONCLUSIONS
  The highest sporulation obtained corresponds to higher antifungal activity when it is formed after 3 days of microbial growth. The antifungal activity against Trichoderma harzianum CCMI 783 is more influenced by the concentration on the nitrogen source than the culture time of incubation. The association of nitrogen concentration and the time of incubation is particularly relevant in the expression of the antifungal activity.
  
  
  CONCLUSIONS
  The present findings allow the reduction of the use of chemical pesticides and to limit some plant diseases. The association of the nitrogen source and the time of incubation is a novelty, which would improve the production of secondary metabolites. Both economical and environmental benefits arise from the study.
authors.affiliation: Universidade de Evora, Departamento de Química, Evora, Portugal.
authors.forename: A T
authors.initials: AT
authors.lastname: Caldeira
authors.forename: S S
authors.initials: SS
authors.lastname: Feio
authors.forename: J M S
authors.initials: JM
authors.lastname: Arteiro
authors.forename: A V
authors.initials: AV
authors.lastname: Coelho
authors.forename: J C
authors.initials: JC
authors.lastname: Roseiro
mesh_terms: D000935:Antifungal Agents / Q000096:biosynthesis / Q000494:pharmacology*; D001407:Bacillus / Q000378:metabolism* / Q000502:physiology; D001431:Bacteriological Techniques; D019149:Bioreactors / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D009584:Nitrogen / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013171:Spores, Bacterial; D014242:Trichoderma / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D009584:Nitrogen