[Research on thymopentin-loaded N-trimethyl chitosan nanoparticles administered through mouth].
OBJECTIVE
To prepare a peroral thymopentin-loaded N-trimethyl chitosan chloride-nanoparticle (Tp5-TMC-NP) ,and observe the pharmacodynamic action when the Tp5-TMC-NP is taken by way of the mouth.


METHODS
N-trimethyl chitosan chloride was first synthesized, and then Tp5-TMC-NP was prepared with the formulation technology optimized by the Central Composite Design. The influence of Tp5-TMC-NP on the ratio of CD4+/CD8+ of T-lymphocytes were determined by flow cytometer.


RESULTS
The regular global Tp5-TMC-NP prepared with the optimized formulation craft had the mean diameter of 110.6 nm and got the entrapment efficiency of 78.8%. The ratio of lymphocyte CD4+/CD8+ of Wistar rat administered with Tp5-TMC-NP perfusing stomach had 2.59 times higher than that with Tp5.


CONCLUSIONS
Taken orally the Tp5-TMC-NP has much higher efficiency than Tp5.
Tang
Shi-wei
SW
Key Laboratory of Drug Targeting, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Yuan
Xiao-jia
XJ
Zhang
Zhi-rong
ZR
Song
Qing-guo
QG
He
Qin
Q
D000284:Administration, Oral; D000818:Animals; D016516:CD4-CD8 Ratio; D048271:Chitosan / Q000008:administration & dosage*; D004337:Drug Carriers / Q000008:administration & dosage*; D053758:Nanoparticles / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D016305:Thymopentin / Q000008:administration & dosage* / Q000493:pharmacokinetics
D004337:Drug Carriers; C118071:N-trimethyl chitosan chloride; D048271:Chitosan; D016305:Thymopentin