Nickel resistance determinants in bradyrhizobium strains from nodules of the endemic New Caledonia legume Serianthes calycina.
Bradyrhizobium strains, isolated in New Caledonia from nodules of the endemic legume Serianthes calycina growing in nickel-rich soils, were able to grow in the presence of 15 mM NiCl2. The genomes of these strains harbored two Ni resistance determinants, the cnr and nre operons. By constructing a cnrA mutant, we demonstrated that the cnr operon determines the high nickel resistance in Bradyrhizobium strains.
Chaintreuil
Clémence
C
LSTM, Campus International de Baillarguet, TA A-82/J, 34398 Montpellier Cedex 5, France. clemence.chaintreuil@mpl.ird.fr
Rigault
Frédéric
F
Moulin
Lionel
L
Jaffré
Tanguy
T
Fardoux
Joël
J
Giraud
Eric
E
Dreyfus
Bernard
B
Bailly
Xavier
X
D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000633:toxicity*; D001426:Bacterial Proteins / Q000235:genetics; D020369:Bradyrhizobium / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D007887:Fabaceae / Q000382:microbiology; D008969:Molecular Sequence Data; D016296:Mutagenesis; D050505:Mutant Proteins / Q000235:genetics; D009510:New Caledonia; D009532:Nickel / Q000378:metabolism / Q000633:toxicity*; D009876:Operon; D017422:Sequence Analysis, DNA
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D050505:Mutant Proteins; C022838:nickel chloride; D009532:Nickel