Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes.
Reverse micellar systems of CTAB/isooctane/hexanol/butanol and AOT/isooctane are used for the extraction and primary purification of bromelain from crude aqueous extract of pineapple wastes (core, peel, crown and extended stem). The effect of forward as well as back extraction process parameters on the extraction efficiency, activity recovery and purification fold is studied in detail for the pineapple core extract. The optimized conditions for the extraction from core resulted in forward and back extraction efficiencies of 45% and 62%, respectively, using reverse micellar system of cationic surfactant CTAB. A fairly good activity recovery (106%) and purification (5.2-fold) of bromelain is obtained under these conditions. Reverse micellar extraction from peel, extended stem and crown using CTAB system resulted in purification folds of 2.1, 3.5, and 1.7, respectively. Extraction from extended stem using anionic surfactant AOT in isooctane did not yield good results under the operating conditions employed.
Umesh Hebbar
H
H
Department of Food Engineering, Central Food Technological Research Institute, Mysore 570 020, India.
Sumana
B
B
Raghavarao
K S M S
KS
D031230:Ananas / Q000187:drug effects / Q000201:enzymology*; D001963:Bromelains / Q000302:isolation & purification*; D001965:Bromides / Q000494:pharmacology; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D045424:Complex Mixtures; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008823:Micelles; D018547:Plant Stems / Q000187:drug effects / Q000201:enzymology; D017680:Potassium Compounds / Q000494:pharmacology; D012037:Refuse Disposal; D012965:Sodium Chloride / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology
D001965:Bromides; D002593:Cetrimonium Compounds; D045424:Complex Mixtures; D008823:Micelles; D017680:Potassium Compounds; D013501:Surface-Active Agents; D012965:Sodium Chloride; D001963:Bromelains; C091760:stem bromelain; C039004:potassium bromide; D000077286:Cetrimonium