Biochemical analysis of inositol phosphate kinases.
Lipid-derived inositol phosphates (IPs) are a complex group of second messengers generated by the sequential phosphorylation of inositol 1,4,5-trisphosphate (IP(3)). Synthetic pathways leading from IP(3) to the formation of inositol tetrakisphosphate IP(4), inositol pentakisphosphate IP(5), inositol hexakisphosphate IP(6), and inositol pyrophosphates PP-IPs have been elucidated in eukaryotes from yeast to human. Studies have attributed a variety of cellular functions to IPs, highlighting the importance of understanding how the pathways for their synthesis are regulated. This chapter summarizes experimental techniques for the biochemical characterization of the key inositol phosphate kinases IPKs necessary for producing the diverse array of IP species.
Otto
James C
JC
Howard Hughes Medical Institute, Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, USA.
Mulugu
Sashidhar
S
Fridy
Peter C
PC
Chiou
Shean-Tai
ST
Armbruster
Blaine N
BN
Ribeiro
Anthony A
AA
York
John D
JD
D017360:Arabidopsis / Q000201:enzymology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D015544:Inositol 1,4,5-Trisphosphate / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007295:Inositol Phosphates / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010766:Phosphorylation; D010770:Phosphotransferases / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
D029681:Arabidopsis Proteins; D007295:Inositol Phosphates; D011994:Recombinant Proteins; D015544:Inositol 1,4,5-Trisphosphate; D010770:Phosphotransferases