t(6;14)(p22;q32): a new recurrent IGH@ translocation involving ID4 in B-cell precursor acute lymphoblastic leukemia (BCP-ALL).
Translocations involving the immunoglobulin heavy chain locus (IGH@) at chromosome band 14q32 are common in mature B-cell neoplasms, but are rare in B-cell precursor acute lymphoblastic leukemia (BCP-ALL). Here, we report the translocation, t(6;14)(p22;q32), involving IGH@ as a novel recurrent translocation in 13 BCP-ALL patients. Fluorescence in situ hybridization and long-distance inverse polymerase chain reaction (PCR) identified ID4 as the partner gene. Breakpoints were scattered over a 19kb region centromeric of ID4. Quantitative real-time PCR showed up-regulation of ID4 mRNA. All patients had deletions of CDKN2A and PAX5 located on the short arm of chromosome 9, frequently as a result of an isochromosome, i(9)(q10) (9/13, 69%). This study defines a new subgroup of BCP-ALL characterized by ID4 over-expression and CDKN2A and PAX5 deletions. Preliminary survival data suggest that this subgroup may be associated with a good response to therapy.
Russell
Lisa J
LJ
Leukaemia Research Cytogenetics Group, Cancer Sciences Division, University of Southampton, Southampton General Hospital, Southampton, United Kingdom.
Akasaka
Takashi
T
Majid
Aneela
A
Sugimoto
Kei-Ji
KJ
Loraine Karran
E
E
Nagel
Inga
I
Harder
Lana
L
Claviez
Alexander
A
Gesk
Stefan
S
Moorman
Anthony V
AV
Ross
Fiona
F
Mazzullo
Helen
H
Strefford
Jonathan C
JC
Siebert
Reiner
R
Dyer
Martin J S
MJ
Harrison
Christine J
CJ
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D002648:Child; D002883:Chromosomes, Human, Pair 14; D002896:Chromosomes, Human, Pair 6; D002899:Chromosomes, Human, Pair 9; D005260:Female; D017353:Gene Deletion; D019942:Genes, p16; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D051796:Inhibitor of Differentiation Proteins / Q000235:genetics*; D015448:Leukemia, B-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D051757:PAX5 Transcription Factor / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic
C490128:ID4 protein, human; D007143:Immunoglobulin Heavy Chains; D051796:Inhibitor of Differentiation Proteins; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human