title: Molecular mechanism of molt-inhibiting hormone (MIH) induced suppression of ecdysteroidogenesis in the Y-organ of mud crab: Scylla serrata.
abstract: The present study was focused on the regulation of ecdysteroidogenesis in the Y-organ of Scylla serrata during molting cycle. A strong expression of molt-inhibiting hormone (MIH) and phosphorylation of ERK was predominantly observed in the postmolt and intermolt stages of Y-organs, whereas protein kinase C, steroidogenic acute regulatory protein (StAR) and cytochrome P450(scc) activity were exclusively seen in the premolt stages. Interestingly, inhibition of ERK phosphorylation by PD98059 in the early postmolt (A), middle postmolt (B) and intermolt (C) stages resulted in the prominent expression of PKC and StAR in the postmolt stages. This result indicates that phosphorylation of ERK is required for suppression of ecdysteroid biosynthesis with the involvement of protein kinase C, and StAR protein.
authors.affiliation: Department of Zoology, University of Madras, Life Sciences Building, Guindy Campus, Chennai 600 025, Tamil Nadu, India.
authors.forename: L
authors.initials: L
authors.lastname: Imayavaramban
authors.forename: D
authors.initials: D
authors.lastname: Dhayaparan
authors.forename: Halagowder
authors.initials: H
authors.lastname: Devaraj
mesh_terms: D000818:Animals; D001055:Apolipoproteins B / Q000378:metabolism; D003386:Brachyura / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D002784:Cholesterol / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D004702:Endocrine Glands / Q000502:physiology*; D005419:Flavonoids / Q000494:pharmacology; D007447:Invertebrate Hormones / Q000502:physiology*; D020935:MAP Kinase Signaling System; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D008954:Models, Biological; D018978:Molting / Q000502:physiology; D009479:Neuropeptides / Q000502:physiology*; D010750:Phosphoproteins / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D001055:Apolipoproteins B; D005419:Flavonoids; D007447:Invertebrate Hormones; D009479:Neuropeptides; D010750:Phosphoproteins; D012333:RNA, Messenger; C012445:molt-inhibiting hormone; D000098682:Steroidogenic Acute Regulatory Protein; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol; D011493:Protein Kinase C; D020928:Mitogen-Activated Protein Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one