[Effects of shock lymph inducing the mesentery micro-lymphatic endothelial cells apoptosis of rats].
The model of serious hemorrhagic shock was established in the condition of asepsis and mesentery lymph was taken out. As control, normal mesentery lymph fluid, normal portal vein blood and shock portal vein blood of rats were taken out. The primary mesentery micro-lymphatic endothelial cells (MMLECs) of passages 3 were treated by different treatment factors, respectively. The cells proliferation by shock lymph and normal lymph of different final concentrations was measured using MTT method. The cell cycle arrest was analyzed by flow cytometry, and the electrophoresis analyze on DNA of cell nucleus was observed. The expressions of relative genes of apoptosis such as fas, fasL, bcl-2 and bax of MMLECs were detected by RT-PCR. The results showed that the MMLECs proliferation was decreased when the concentration of shock lymph increased to some extent and showed statisticly significant difference compared with normal lymph group. The cells cocultured with shocking lymph fluid at 4% final concentration showed that apoptosis rate of MMLECs was (35.4 +/- 1.6)%, and G0-G1 cell population was higher and the proportion of S+G2-M cell population was lower than that of other groups, and the DNA ladder was observed in electrophoresis of cell nucleus DNA at same time. And the expressions level of fas, fasL and bax mRNA were higher and bcl-2 mRNA was lower in shock lymph group than that of control group. The results demonstrated that shock lymph could reduce the cells proliferation, interfere with cell cycle, and accelerate high expression of apoptosis accelerative genes. As a result, shock lymph could induce the MMLECs apoptosis.
Niu
Chun Yu
CY
Department of Pathophysiololgy, Hebei North University, Zhangfiakou 075029, China. ncylxf@126.com
Zhao
Zi Gang
ZG
Chen
Rui Hua
RH
Li
Ji Cheng
JC
Zhang
Jing
J
Liu
Yan Kai
YK
Zhang
Yu Ping
YP
D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D002453:Cell Cycle; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D015870:Gene Expression; D008196:Lymph / Q000166:cytology / Q000378:metabolism; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012771:Shock, Hemorrhagic / Q000503:physiopathology*; D051028:bcl-2-Associated X Protein / Q000235:genetics
C494828:Bax protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein