title: Mutagenesis of the phosphate-binding pocket of KDPG aldolase enhances selectivity for hydrophobic substrates.
abstract: Narrow substrate specificities often limit the use of enzymes in biocatalysis. To further the development of Escherichia coli 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase as a biocatalyst, the molecular determinants of substrate specificity were probed by mutagenesis. Our data demonstrate that S184 is located in the substrate-binding pocket and interacts with the phosphate moiety of KDPG, providing biochemical support for the binding model proposed on the basis of crystallographic data. An analysis of the substrate selectivity of the mutant enzymes indicates that alterations to the phosphate-binding site of KDPG aldolase changes the substrate selectivity. We report mutations that enhance catalysis of aldol cleavage of substrates lacking a phosphate moiety and demonstrate that electrophile reactivity correlates with the hydrophobicity of the substituted side chain. These mutations improve the selectivity for unnatural substrates as compared to KDPG by up to 2000-fold. Furthermore, the S184L KDPG aldolase mutant improves the catalytic efficiency for the synthesis of a precursor for nikkomycin by 40-fold, making it a useful biocatalyst for the preparation of fine chemicals.
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor 48109, USA.
authors.forename: Manoj
authors.initials: M
authors.lastname: Cheriyan
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Toone
authors.forename: Carol A
authors.initials: CA
authors.lastname: Fierke
mesh_terms: D000446:Aldehyde-Lyases / Q000737:chemistry* / Q000378:metabolism; D000617:Aminoglycosides / Q000737:chemistry; D001665:Binding Sites; D002384:Catalysis; D004926:Escherichia coli / Q000378:metabolism; D006836:Hydro-Lyases / Q000737:chemistry; D007700:Kinetics; D008956:Models, Chemical; D008958:Models, Molecular; D008968:Molecular Conformation; D016296:Mutagenesis; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D010710:Phosphates / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D013379:Substrate Specificity
chemical_list: D000617:Aminoglycosides; D010710:Phosphates; C011952:nikkomycin; D000446:Aldehyde-Lyases; C100131:phospho-2-keto-3-deoxy-gluconate aldolase; D006836:Hydro-Lyases; C022362:phosphogluconate dehydratase