The rapid identification of Clostridium perfringens as the possible aetiology of a diarrhoeal outbreak using PCR.
A gastroenteritis outbreak occurred in a military camp where a laboratory and epidemiological investigation was carried out. The early onset of symptoms indicated probable food contamination with Clostridium perfringens. Stool samples collected from affected patients were tested within 4 h via real-time polymerase chain reaction (PCR) for the presence of the C. perfringens plc gene. Ten out of the 12 stool samples were positive. Confirmation of the molecular test results was carried out by enumeration of C. perfringens in stool by culture and shown to be in excess of 106 spores/g stool. The isolates obtained from culture were further analysed by PCR for the presence of the chromosomal enterotoxin (cpe) gene. Based on the clinical symptoms, epidemiological and laboratory investigations, C. perfringens was implicated as the aetiological agent. The ability to conduct real-time PCR analysis greatly shortens the time to diagnosis and allows for preventive and control measures to be effected quickly.
Loh
J P
JP
Defence Medical and Environmental Research Institute, DSO National Laboratories, Singapore. jimmyloh@dso.org.sg
Liu
Y C
YC
Chew
S W
SW
Ong
E S
ES
Fam
J M
JM
Ng
Y Y
YY
Taylor
M B
MB
Ooi
E E
EE
D003015:Clostridium Infections / Q000453:epidemiology* / Q000382:microbiology; D003016:Clostridium perfringens / Q000145:classification* / Q000302:isolation & purification; D003967:Diarrhea / Q000382:microbiology*; D004196:Disease Outbreaks; D004768:Enterotoxins / Q000032:analysis; D005243:Feces / Q000382:microbiology; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012846:Singapore / Q000453:epidemiology
D004768:Enterotoxins; C035598:enterotoxin, Clostridium