title: Webtag: a new web tool providing tags/anchors for RT-PCR experiments with prokaryotes.
abstract: BACKGROUND
  Webtag is a tool providing oligonucleotide sequences (usually called tags or anchors) that are absent from a specified genome. These tags/anchors can be appended to gene specific primers for reverse transcriptase polymerase chain reaction experiments, circumventing genomic DNA contamination.
  
  
  RESULTS
  The use of a relational database, in conjunction with a series of scripts written in PHP and Perl, allows the user to rapidly obtain tags that are: 1) suitable for a specific organism, and 2) compatible with other oligonucleotides to be used in the experimental procedures.
  
  
  CONCLUSIONS
  This new web tool allows scientists to easily and rapidly obtain suitable tags for RT-PCR experiments, and is available at http://www.egs.uu.se/software/webtag/.
authors.affiliation: Department of Photochemistry and Molecular Science, The Angström Laboratories, Uppsala University, Box 523, SE-75120, Uppsala, Sweden. fernando.lopespinto@fotomol.uu.se
authors.forename: Fernando Lopes
authors.initials: FL
authors.lastname: Pinto
authors.forename: Håkan
authors.initials: H
authors.lastname: Svensson
authors.forename: Peter
authors.initials: P
authors.lastname: Lindblad
mesh_terms: D000465:Algorithms; D001483:Base Sequence; D030541:Databases, Genetic; D009841:Oligonucleotides / Q000235:genetics; D011387:Prokaryotic Cells / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D016324:Sequence Tagged Sites; D012984:Software
chemical_list: D009841:Oligonucleotides