The murine pan T cell marker CD96 is an adhesion receptor for CD155 and nectin-1.
The CD155 ligand CD96 is an immunoglobulin-like protein tentatively allocated to the repertoire of human NK receptors. We report here that the CD96/CD155-interaction is preserved between man and mouse although both receptors are only moderately conserved in amino acid sequence. Moreover, murine CD96 (mCD96) binds to nectin-1, a receptor related to CD155. Applying newly generated monoclonal antibodies specifically recognizing mCD96, an expression profile is revealed resembling closely that of human CD96 (hCD96) on cells of hematopoietic origin. A panel of anti-mCD96 but also recently established anti-mCD155 antibodies effectively prevents formation of CD96/CD155-complexes. This was exploited to demonstrate that the only available receptor for mCD96 present on thymocytes is mCD155. Moreover, T cell adhesion to insect cells expressing mCD155 is blocked by these antibodies depending on the T cell subtype. These results suggest a function of the CD96/CD155-adhesion system in T cell biology.
Seth
S
S
Institute of Immunology, Hannover Medical School, Carl-Neuberg Str. 1, 30625 Hannover, Germany.
Maier
M K
MK
Qiu
Q
Q
Ravens
I
I
Kremmer
E
E
Förster
R
R
Bernhardt
G
G
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D000075983:Nectins; D019038:Platelet Glycoprotein GPIb-IX Complex; D051381:Rats; D011991:Receptors, Virus / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
D015703:Antigens, CD; C116131:CD96 antigen; D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C000619010:NECTIN1 protein, human; C000619015:Nectin1 protein, mouse; D000075983:Nectins; D019038:Platelet Glycoprotein GPIb-IX Complex; D011991:Receptors, Virus; C094990:adhesion receptor; C066100:poliovirus receptor