title: [Separation and identification of chlorogenic acid and related impurities by high performance liquid chromatography-tandem mass spectrometry].
abstract: A method using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established for analysis and identification of chlorogenic acid along with its related impurities. A Gemini C18 HPLC column (4.6 mm x 150 mm,5 microm) was used with acetonitrile-water ( containing 0.1% formic acid) (8:92, v/v) as mobile phase. Eight related impurities of chlorogenic acid were identified and their structures were determined by using online HPLC-MS/MS and photodiode array detector (DAD). The method is quick, simple and can be used directly to identify the structure of unknown trace substances in the sample of chlorogenic acid.
authors.affiliation: West China Pharmaceutical School of Sichuan University, Chengdu 610041, China.
authors.forename: Chenxu
authors.initials: C
authors.lastname: Tian
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Xu
authors.forename: Liyun
authors.initials: L
authors.lastname: Liao
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Sha
authors.initials: S
authors.lastname: Zhou
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002726:Chlorogenic Acid / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014867:Water / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D014867:Water; D002726:Chlorogenic Acid; C032159:acetonitrile