[Effects of EPA, DHA on the secretion of NO, expression of iNOS mRNA and DNA-binding activity of NFkappaB in human monocyte].
OBJECTIVE
To study the effect of n-3 polyunsaturated fatty acids (EPA, DHA) on the production of NO, expression of iNOS mRNA and DNA-binding activity of NFkappaB in human monocyte.


METHODS
RT-PCR and enzyme reduction method were used to measure the expression of iNOS mRNA and production of NO respectively. The DNA-binding activity of NFkappaB was assessed by EMSA.


RESULTS
EPA, DHA can decrease the production of NO, expression of iNOS mRNA and DNA-binding activity of NFkappaB between the dosage of 10microg/ml and 20microg/ml in human peripheral blood monocyte in vitro.


CONCLUSIONS
EPA, DHA have inhibitory effects on NO production, iNOS mRNA expression and DNA-binding activity of NFkappaB in monocyte. It indicates EPA, DHA suppress the production of inflammatory mediators via the NFkappaB signal transduction pathways in human monocyte, accordingly suppress inflammatory responses.
Xia
Yanping
Y
Faculty of Medical Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
Feng
Xiang
X
Chen
Yuming
Y
Su
Yixiang
Y
D004247:DNA / Q000378:metabolism; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D006801:Humans; D009000:Monocytes / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
D016328:NF-kappa B; D012333:RNA, Messenger; D004281:Docosahexaenoic Acids; D009569:Nitric Oxide; D004247:DNA; D015118:Eicosapentaenoic Acid; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II