Assessment of gelatinase expression and activity in articular cartilage.
Two methods for the assessment of the expression of gelatinases A and B, MMP-2 and MMP-9, in articular cartilage are described. Immunohistochemical analysis of tissue sections provides information about the precise localization of the enzymes within the tissue, pinpointing the cells that synthesize the proteinases, and zymography of cell/ tissue conditioned culture media allows a semi-quantitative assessment of the gelatinases and their activation status.
Hembry
Rosalind M
RM
Atkinson
Susan J
SJ
Murphy
Gillian
G
D000818:Animals; D002358:Cartilage, Articular / Q000201:enzymology*; D017077:Culture Media, Conditioned; D019084:Fluorescent Antibody Technique, Indirect; D005629:Frozen Sections; D005780:Gelatin; D018093:Gelatinases / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D013552:Swine
D017077:Culture Media, Conditioned; D005780:Gelatin; D018093:Gelatinases; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9