Different apolipoproteins impact nanolipoprotein particle formation.
Spontaneous interaction of purified apolipoproteins and phospholipids results in formation of lipoprotein particles with nanometer-sized dimensions; we refer to these assemblies as nanolipoprotein particles or NLPs. These bilayer constructs can serve as suitable mimetics of biological membranes and are fully soluble in aqueous environments. We made NLPs from dimyristoylphospatidylcholine (DMPC) in combination with each of four different apolipoproteins: apoA-I, Delta-apoA-I fragment, apoE4 fragment, and apolipophorin III (apoLp-III) from the silk moth B. mori. Predominately discoidal in shape, these particles have diameters between 10 and 20 nm, share uniform heights between 4.5 and 5 nm, and can be produced in yields ranging between 40 and 60%. The particular lipoprotein, the lipid to lipoprotein ratio, and the assembly parameters determine the size and homogeneity of nanolipoprotein particles and indicate that apoA-I NLP preparations are smaller than the larger apoE422K and apoLp-III NLP preparations.
Chromy
Brett A
BA
Chemistry, Materials, and Life Sciences, Lawrence Livermore National Laboratory, Livermore, California 94551, USA.
Arroyo
Erin
E
Blanchette
Craig D
CD
Bench
Graham
G
Benner
Henry
H
Cappuccio
Jenny A
JA
Coleman
Matthew A
MA
Henderson
Paul T
PT
Hinz
Angie K
AK
Kuhn
Edward A
EA
Pesavento
Joseph B
JB
Segelke
Brent W
BW
Sulchek
Todd A
TA
Tarasow
Ted
T
Walsworth
Vicki L
VL
Hoeprich
Paul D
PD
D000818:Animals; D016632:Apolipoprotein A-I / Q000737:chemistry / Q000378:metabolism; D053327:Apolipoprotein E4 / Q000737:chemistry / Q000378:metabolism; D001053:Apolipoproteins / Q000737:chemistry* / Q000378:metabolism; D002845:Chromatography; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008074:Lipoproteins / Q000737:chemistry* / Q000378:metabolism; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D009036:Moths / Q000737:chemistry* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010743:Phospholipids / Q000737:chemistry* / Q000378:metabolism
D016632:Apolipoprotein A-I; D053327:Apolipoprotein E4; D001053:Apolipoproteins; D008074:Lipoproteins; D010743:Phospholipids; C042470:apolipophorin III; D004134:Dimyristoylphosphatidylcholine