Inhibition of interleukin-2 production by myricetin in mouse EL-4 T cells.
Myricetin is a naturally occurring flavonoid that is commonly found in tea, berries, fruits, vegetables, and medicinal herbs. This study examined the effects of myricetin on the production of interlukin-2 (IL-2), a potent T cell growth factor. Treatment with myricetin significantly inhibited the secretion of the IL-2 protein from mouse EL-4 T cells activated with phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io) in a dose-dependent manner. Flow cytometric analysis showed that myricetin suppressed the intracellular production of the IL-2 protein. Furthermore, the effects of myricetin on mRNA expression were analyzed by reverse transcription-polymerase chain reaction and it showed that myricetin reduced the expression of IL-2 mRNA induced by PMA plus Io. This suggests that myricetin has potential immunosuppressive effects by inhibiting the production of IL-2.
Cho
Young-Chang
YC
College of Pharmacy and Drug Development Research Institute, Chonnam National University, Gwangju 500-757, Korea.
Yoon
Goo
G
Lee
Kwang Youl
KY
Choi
Hyun Jin
HJ
Kang
Bok Yun
BY
D000818:Animals; D002478:Cells, Cultured; D005419:Flavonoids / Q000494:pharmacology*; D007376:Interleukin-2 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D016328:NF-kappa B / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D005419:Flavonoids; D007376:Interleukin-2; D016328:NF-kappa B; D012333:RNA, Messenger; C040015:myricetin; D013755:Tetradecanoylphorbol Acetate