Separation of proteins by blue native electrophoresis.
Blue native gel electrophoresis is a native electrophoresis method that can be used for molecular weight determination for most soluble protein complexes as well as for most membrane proteins. Subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) can be used in a second dimension to resolve the complexes into their subunits. The method has been extensively used for the analysis of the respiratory chain complexes, for the determination of intermediates of mitochondrial protein import, and for the identification of the composition of the protein import machinery for mitochondria and chloroplasts. Here we describe the basic method and some applications in the research of mitochondrial protein import.
Randelj
Olga
O
Institut für Physiologische Chemie, Ruhr-Universität Bochum, Bochum, Germany.
Rassow
Joachim
J
Motz
Christian
C
D002352:Carrier Proteins / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008565:Membrane Proteins / Q000302:isolation & purification*; D024101:Mitochondrial Proteins / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism; D012995:Solubility
D002352:Carrier Proteins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins