title: ephrinB1 signals from the cell surface to the nucleus by recruitment of STAT3.
abstract: The Eph (erythropoietin-producing hepatoma) family of receptor tyrosine kinases and their membrane-bound ligands, the ephrins, have been implicated in regulating cell adhesion and migration during development by mediating cell-to-cell signaling events. The transmembrane ephrinB (Eph receptor interactor B) protein is a bidirectional signaling molecule that sends a forward signal through the activation of its cognate receptor tyrosine kinase, residing on another cell. A reverse signal can be transduced into the ephrinB-expressing cell via tyrosine phosphorylation of its conserved C-terminal cytoplasmic domain. Although some insight has been gained regarding how ephrinB may send signals affecting cytoskeletal components, little is known about how ephrinB1 reverse signaling affects transcriptional processes. Here we report that signal transducer and activator of transcription 3 (STAT3) can interact with ephrinB1 in a phosphorylation-dependent manner that leads to enhanced activation of STAT3 transcriptional activity. This activity depends on the tyrosine kinase Jak2, and two tyrosines within the intracellular domain of ephrinB1 are critical for the association with STAT3 and its activation. The recruitment of STAT3 to ephrinB1, and its resulting Jak2-dependent activation and transcription of reporter targets, reveals a signaling pathway from ephrinB1 to the nucleus.
authors.affiliation: Laboratory of Cell and Developmental Signaling and Mouse Cancer Genetics Program, National Cancer Institute, Frederick, MD 21702, USA.
authors.forename: Yong-Sik
authors.initials: YS
authors.lastname: Bong
authors.forename: Hyun-Shik
authors.initials: HS
authors.lastname: Lee
authors.forename: Laura
authors.initials: L
authors.lastname: Carim-Todd
authors.forename: Kathleen
authors.initials: K
authors.lastname: Mood
authors.forename: Tagvor G
authors.initials: TG
authors.lastname: Nishanian
authors.forename: Lino
authors.initials: L
authors.lastname: Tessarollo
authors.forename: Ira O
authors.initials: IO
authors.lastname: Daar
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D004247:DNA / Q000378:metabolism; D036387:Ephrin-B1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014158:Transcription, Genetic / Q000235:genetics; D014982:Xenopus laevis
chemical_list: D036387:Ephrin-B1; D050796:STAT3 Transcription Factor; D019000:Phosphotyrosine; D004247:DNA; D053614:Janus Kinase 2