Synthesis and in vivo evaluation of 99m Tc-Transferrin conjugate for detection of inflamed site.
Transferrin is one of acute phase reactants in inflamed lesion. Expression of transferrin receptor is increased in activated macrophage during inflammation process. Conjugates of target ligand with novel water-soluble chitosan for fast excretion of background radioactivity have been shown to be selectively taken up via the target molecules. In this study, radiolabeled 99m Tc-Transferrin conjugate was synthesized and evaluated its efficacy in vivo as a targeted agent for the rapid detection of inflamed lesion that expresses relatively high level of transferrin receptors. Transferrin was conjugated with HYNIC-chitosan and radiolabeled with 99m Tc. The biodistribution and scintigraphic images with the 99m Tc-HYNIC-chitosan-Transferrin conjugate (99m Tc-Tfc) were studied in a murine infection model in which the infection was induced with Escherichia coli (2 x 10(6) colonies). The %ID/g was as follows: 1.612 +/- 0.098, 2.473 +/- 0.202 and 2.617 +/- 0.646% at 30, 120 min and 6 h after injection, respectively. Gamma camera imaging rapidly visualized the infection/inflammation lesion, with the lesion-to-background ratio improving with time up to 5.68 +/- 0.48. 99m Tc-Tfc scintigraphy allows rapid and good imaging of an inflamed lesion.
Kim
Eun-Mi
EM
Department of Nuclear Medicine, Research Institute of Clinical Medicine, Institute for Medical Sciences, Chonbuk National University Medical School and Hospital, Jeonju, Chonbuk, South Korea.
Jeong
Hwan-Jeong
HJ
Kim
Se-Lim
SL
Lee
Chang-Moon
CM
Kim
Dong Wook
DW
Lim
Seok Tae
ST
Sohn
Myung Hee
MH
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D005260:Female; D007249:Inflammation / Q000000981:diagnostic imaging*; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D015609:Organotechnetium Compounds / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D011877:Radionuclide Imaging; D014018:Tissue Distribution; D014168:Transferrin / Q000138:chemical synthesis* / Q000493:pharmacokinetics
D015609:Organotechnetium Compounds; D014168:Transferrin