Haeamtang induces apoptosis of colon cancer HT-29 cells through activation of caspase-3.
The effect of Haeamtang (HAT) on the colon cancer HT-29 cells was investigated in this study. A water extract of HAT significantly decreased the number of HT-29 cells in a dose-and time-dependent manner as determined by a MTT assay. Flow cytometry results revealed a dose- and time-dependent increase of dead cells in HT-29 cells treated with HAT extract. The anticancer activity of the H AT extract is attributed to apoptosis induced in HT-29 cells, which was demonstrated by increased caspase-3 activity and poly-ADP-ribose polymerase fragmentation. A selective caspase inhibitor, z-VAD-fmk, inhibited the HAT-induced cell death. Taken together, these results demonstrate that HAT extract induces apoptosis in HT-29 cells.
Moon
Phil-Dong
PD
Cancer Preventive Material Development Research Center, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul, 130-701, Republic of Korea.
Koo
Hyun-Na
HN
Jeong
Hyun-Ja
HJ
Na
Ho-Jeong
HJ
Kim
Su-Jin
SJ
Hwang
Gab-Soo
GS
Han
Na-Ra
NR
Hong
Seung-Heon
SH
Kim
Hyung-Min
HM
Um
Jae-Young
JY
D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism*; D004789:Enzyme Activation; D005434:Flow Cytometry; D019073:HT29 Cells; D006801:Humans; D008519:Medicine, Traditional; D010936:Plant Extracts
C526215:Haeamtang; D010936:Plant Extracts; D053148:Caspase 3