Interaction of a new fluorescent probe with DNA and its use in determination of DNA.
In this paper we reported a metal complex 1-Zn (2,5-di-[2-(3,5-bis(2-pyridylmethyl)amine-4-hydroxy-phenyl)-ethylene]-pyrazine-Zn) as a fluorescent probe sensing DNA. The result of the competitive experiment of the probe with ethidium bromide (EB) to bind DNA, absorption spectral change and polarization change in the presence and absence of DNA revealed that interaction between the probe and DNA was via intercalation. Ionic strength experiment showed the existence of electrostatic interaction as well. Scatchard plots also confirmed the combined binding modes. The fluorescence enhancement of the probe was ascribed to highly hydrophobic environment when it bound the macromolecules such as DNA, RNA or denatured DNA. The binding constant between the probe and DNA was estimated as 3.13 x 10(7) mol(-1) L. The emission intensity increase was proportional to the concentration of DNA. Based on this, the probe was used to determine the concentration of calf thymus DNA (ct-DNA). The corresponding linear response ranged from 2.50 x 10(-7) to 4.75 x 10(-6) mol L(-1), and detection limit was 1.93 x 10(-8) mol L(-1) for ct-DNA.
Wu
Fang-Ying
FY
Department of Chemistry, Nanchang University, Nanchang 330031, China. fywu@ncu.edu.cn
Xie
Fei-Yan
FY
Wu
Yu-Mei
YM
Hong
Jong-In
JI
D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D004996:Ethidium / Q000737:chemistry / Q000378:metabolism; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
C528474:2,5-di-(2-(3,5-bis(2-pyridylmethyl)amine-4-hydroxy-phenyl)-ethylene)-pyrazine-Zn; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium