Estimating the distribution of the G(2) phase duration from flow cytometric histograms.
A mathematical model, based on branching processes, is proposed to interpret BrdUrd DNA FCM-derived data. Our main interest is in determining the distribution of the G(2) phase duration. Two different model classes involving different assumptions on the distribution of the G(2) phase duration are considered. Different assumptions of the G(2) phase duration result in very similar distributions of the S phase duration and the estimated means and standard deviations of the G(2) phase duration are all in the same range.
Larsson
Sara
S
Centre for Mathematical Sciences, Division of Mathematical Statistics, Lund University, 221 00, Lund, Sweden. sara@maths.lth.se
Ryden
Tobias
T
Holst
Ulla
U
Oredsson
Stina
S
Johansson
Maria
M
D000465:Algorithms; D001973:Bromodeoxyuridine / Q000032:analysis / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology; D045744:Cell Line, Tumor; D003198:Computer Simulation; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D005434:Flow Cytometry / Q000379:methods*; D016195:G2 Phase / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D016013:Likelihood Functions; D008954:Models, Biological; D016196:S Phase / Q000502:physiology; D016008:Statistical Distributions; D013269:Stochastic Processes
D004247:DNA; D001973:Bromodeoxyuridine