Enrichment of Yersinia pestis from blood cultures enables rapid antimicrobial susceptibility determination by flow cytometry.
Mortality from plague is high if not treated with the proper antibiotics within 18-24 hours after onset of symptoms. The process of antibiotic susceptibility determination of Yersinia pestis isolated from blood samples may extend from 4 to more than 7 days, since the in vitro growth is very slow. To accelerate this process, we developed an enrichment protocol as well as a non-standard yet reliable method for rapid antibiotic susceptibility analysis of Y. pestis from blood cultures using flow cytometry technology. This rapid method is applicable to blood cultures containing low levels of Y. pestis.
Steinberger-Levy
Ida
I
Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona.
Zahavy
Eran
E
Cohen
Sara
S
Flashner
Yehuda
Y
Mamroud
Emanuelle
E
Aftalion
Moshe
M
Gur
David
D
Ber
Raphael
R
D016470:Bacteremia / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology; D001431:Bacteriological Techniques; D001769:Blood / Q000382:microbiology; D015169:Colony Count, Microbial; D005434:Flow Cytometry; D006801:Humans; D008826:Microbial Sensitivity Tests; D010930:Plague / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D015010:Yersinia pestis / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification*