title: Noninvasive assessment of the role of cyclooxygenases in cardiovascular health: a detailed HPLC/MS/MS method.
abstract: A robust method for the routine quantitation of a selected group of urinary eicosanoid metabolites of interest to cardiovascular research in human and mouse is described and discussed. Included are the addition of stable isotope-labeled internal standards, solid phase extraction, and quantitation by liquid chromatography/tandem mass spectrometry using selected reaction monitoring (SRM) techniques.
authors.affiliation: Institute for Translational Medicine and Therapeutics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors.forename: Wen-Liang
authors.initials: WL
authors.lastname: Song
authors.forename: John A
authors.initials: JA
authors.lastname: Lawson
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.forename: Helen
authors.initials: H
authors.lastname: Zou
authors.forename: Garret A
authors.initials: GA
authors.lastname: FitzGerald
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism / Q000652:urine; D002318:Cardiovascular Diseases / Q000201:enzymology / Q000652:urine; D002319:Cardiovascular System / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015777:Eicosanoids / Q000378:metabolism* / Q000592:standards / Q000652:urine; D006801:Humans; D051379:Mice; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D015415:Biomarkers; D015777:Eicosanoids; D011451:Prostaglandin-Endoperoxide Synthases