Differential interleukin-6 messenger ribonucleic acid expression and its distribution pattern in eutopic and ectopic endometrium.
OBJECTIVE
To evaluate the concentration and distribution pattern of interleukin-6-producing cells in ectopic and eutopic endometrium.


METHODS
Collection of both tissues at the proliferative cycle phase.


METHODS
A university endocrinology and laparoscopy program.


METHODS
Twelve samples from six women were obtained at operative laparoscopy in the proliferative phase of each woman's cycle.


METHODS
Paired sampling of ectopic (n = 6) and eutopic endometrium (n = 6).


METHODS
Concentration of IL-6 messenger RNA (mRNA) was measured by real-time RT-PCR, and its distribution was determined by in situ RT-PCR.


RESULTS
In vitro RT-PCR analysis showed distinct bands at 234 bp and 115 bp for IL-6 mRNA in eutopic and ectopic endometrium and in placenta as positive control. Real-time RT-PCR indicated significantly higher expression of IL-6 in tissue with endometriosis than in tissue without. In all 12 samples subjected to in situ RT-PCR, positive signals of IL-6 mRNA were observed in cytoplasm of epithelial and stroma cells of ectopic and eutopic endometrium tissue.


CONCLUSIONS
The significant IL-6 mRNA increase in endometriosis compared with in eutopic endometrium may play a role in the development of endometriosis and may provide new directions for endometriosis research.
Salmassi
Ali
A
Department of Obstetrics and Gynecology, University Hospitals Schleswig-Holstein, Campus Kiel, Kiel, Germany. endo-office@email.uni-kiel.des
Acil
Yahya
Y
Schmutzler
Andreas Gerd
AG
Koch
Kerstin
K
Jonat
Walter
W
Mettler
Liselotte
L
D000328:Adult; D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution
D015850:Interleukin-6; D012333:RNA, Messenger