Spectral diffusion of the lowest exciton component in the core complex from Rhodopseudomonas palustris studied by single-molecule spectroscopy.
We have recorded fluorescence-excitation spectra from individual RC-LH1 complexes from Rhodopseudomonas palustris. The spectra feature a few broad bands accompanied by a sharp line at the low-energy side of the spectrum which is ascribed to the lowest exciton state of the BChl a assembly. Recording several fluorescence-excitation spectra from the same individual complex in rapid succession reveals that the linewidth of the lowest exciton transition is determined by spectral diffusion which increases for higher excitation energies.
Richter
Martin F
MF
Experimental Physics IV and Bayreuth Institute of Macromolecular Research (BIMF), University of Bayreuth, 95440, Bayreuth, Germany.
Baier
Jürgen
J
Southall
June
J
Cogdell
Richard J
RJ
Oellerich
Silke
S
Köhler
Jürgen
J
D003080:Cold Temperature; D012241:Rhodopseudomonas / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*