The extent of periodontal disease and the IL-6 -174 genotype as determinants of serum IL-6 level.
OBJECTIVE
To study the extent of periodontal disease and the IL-6(-174) genotype as determinants of serum and mouthwash IL-6 concentration in subjects with moderate to severe periodontal disease.


METHODS
Fifty-two generally healthy subjects volunteered to participate. Probing pocket depth (PD) and periodontal attachment level (AL) were clinically examined and alveolar bone level (BL) was measured on orthopantomographs. IL-6 concentrations in mouthwash, collected by rinsing with 3 ml saline for 30 s and in serum, obtained by venipuncture, were measured using ELISA. IL-6(-174) polymorphism was studied using a polymerase chain reaction.


RESULTS
Eleven subjects carried the GG genotype, and 41 subjects, carried the CG/CC genotype. The mean (+/- SD) concentration of IL-6 in serum was 1.6 (+/- 1.5) pg/ml and, 2.8 (+/- 5.04) pg/ml in mouthwash. The serum concentration of IL-6 was higher in subjects with the GG genotype than with the CG/CC genotype. In regression analyses the percentages of sites with PD> or =6 mm, AL> or =6 mm and BL> or =8 mm, the IL-6(-174) genotype, body mass index and gender associated significantly with serum IL-6 concentration.


CONCLUSIONS
The extent of moderate to severe periodontal disease and the IL-6(-174) genotype contribute significantly to serum IL-6 concentration.
Raunio
Taina
T
The Dental Specialist Clinic of Oulu Municipal Health Center, Oulu, Finland.
Nixdorf
Marianne
M
Knuuttila
Matti
M
Karttunen
Riitta
R
Vainio
Olli
O
Tervonen
Tellervo
T
D000328:Adult; D004812:Epidemiologic Methods; D005260:Female; D005838:Genotype; D006801:Humans; D015850:Interleukin-6 / Q000097:blood* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009067:Mouthwashes / Q000032:analysis / Q000737:chemistry; D010510:Periodontal Diseases / Q000097:blood* / Q000000981:diagnostic imaging / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics*; D011859:Radiography; D012907:Smoking / Q000097:blood
D015850:Interleukin-6; D009067:Mouthwashes