Spectrofluorimetric study and detection of ketoconazole in the presence of beta-cyclodextrin.
The formation of a complex between ketoconazole and beta-cyclodextrin was followed by spectrofluorimetry. The inclusion of ketoconazole in beta-cyclodextrin cavity enhanced the native fluorescence of the drug. The stoichiometry of the complex was 1:1 beta-cyclodextrin to ketoconazole and the stability constant of the complex (log K(f)) was determined to be 4.3+/-0.01 at pH=7.9 and 3.7+/-0.04 at pH=2.6. A sensitive spectrofluorimetric method for the detection of ketoconazole is presented. At optimized experimental conditions, a linear relationship between the fluorescence intensity of the solution and concentration of ketoconazole is observed in the range of 0.01-10 microg ml(-1) (5 x 10(-8) M-1.88 x 10(-5) M). The method was applied to the detection of ketoconazole in pharmaceutical products and the results were satisfactory in comparison to the official method (relative error=2.8% and standard deviation=0.06 for tablets of ketoconazole). The recovery of ketoconazole from a blood serum sample, determined by the proposed method, was 97.1+/-2.4%.
Jalali
F
F
Department of Chemistry, Faculty of Science, Razi University, Kermanshah, Iran. fahimehjalali@yahoo.com
Afshoon
A
A
D000935:Antifungal Agents / Q000737:chemistry*; D002273:Carcinogens / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007654:Ketoconazole / Q000737:chemistry*; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000032:analysis; D013050:Spectrometry, Fluorescence; D047392:beta-Cyclodextrins / Q000737:chemistry* / Q000378:metabolism
D000935:Antifungal Agents; D002273:Carcinogens; D004364:Pharmaceutical Preparations; D047392:beta-Cyclodextrins; C031215:betadex; D007654:Ketoconazole