[Simultaneous determination of nine beta2-agonists residues in urines by ultra performance liquid chromatography-electrospray tandem mass spectrometry].
OBJECTIVE
To study the determination method of nine beta2-agonists residues in urine.


METHODS
Urine samples were duconjugated with beta-glucuronidase/arylsulfatase enzyme in acetate buffer and deproteinized by perchloric acid, and then adjusted pH4.0. Sample concentration and purification were performed by Oasis HLB, Oasis MCX. The separation was performed on a Waters ACQUITY UPLCTM BEH C18 column (100mm x 2.1mm i.d., 1.7microm) with gradient elution using methanol and water (containing 0.1% formic acid) at a flow rate of 0.3ml/min.


RESULTS
The limits of detection (LOD) of the method were from 0.002 to 0.025ng/ml and the limits of quantification (LOQ) ranged from 0.007 to 0.08ng/ml. Average recoveries for nine beta2-agonists at the spiking levels of 0.1, 0.5 and 2ng/ml ranged from 77.4% to 101.7% with relative standard deviations between 3.4% and 18% .


CONCLUSIONS
The method can be used to determine the residues of nine beta2-agonists in urines.
Jia
Xiaofei
X
Chemistry Department of Tianjin University, Tianjin 300072, China.
Qu
Jianqiang
J
Meng
Juan
J
Shao
Bing
B
D000318:Adrenergic beta-Agonists / Q000652:urine*; D000420:Albuterol / Q000652:urine; D006801:Humans; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013726:Terbutaline / Q000031:analogs & derivatives / Q000652:urine
D000318:Adrenergic beta-Agonists; C010809:tulobuterol; D013726:Terbutaline; D000420:Albuterol