Necdin mediates skeletal muscle regeneration by promoting myoblast survival and differentiation.
Regeneration of muscle fibers that are lost during pathological muscle degeneration or after injuries is sustained by the production of new myofibers. An important cell type involved in muscle regeneration is the satellite cell. Necdin is a protein expressed in satellite cell-derived myogenic precursors during perinatal growth. However, its function in myogenesis is not known. We compare transgenic mice that overexpress necdin in skeletal muscle with both wild-type and necdin null mice. After muscle injury the necdin null mice show a considerable defect in muscle healing, whereas mice that overexpress necdin show a substantial increase in myofiber regeneration. We also find that in muscle, necdin increases myogenin expression, accelerates differentiation, and counteracts myoblast apoptosis. Collectively, these data clarify the function and mechanism of necdin in skeletal muscle and show the importance of necdin in muscle regeneration.
Deponti
Daniela
D
Department of Histology and Medical Embryology, University of Roma-La Sapienza, 00161 Rome, Italy.
François
Stéphanie
S
Baesso
Silvia
S
Sciorati
Clara
C
Innocenzi
Anna
A
Broccoli
Vania
V
Muscatelli
Françoise
F
Meneveri
Raffaella
R
Clementi
Emilio
E
Cossu
Giulio
G
Brunelli
Silvia
S
D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D002459:Cell Fusion; D002470:Cell Survival; D002478:Cells, Cultured; D051379:Mice; D008822:Mice, Transgenic; D018485:Muscle Fibers, Skeletal / Q000166:cytology; D018482:Muscle, Skeletal / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D017570:MyoD Protein / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology*; D018008:Myogenin / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000378:metabolism*; D009687:Nuclear Proteins / Q000172:deficiency / Q000378:metabolism*; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology; D015533:Transcriptional Activation
D017570:MyoD Protein; D018008:Myogenin; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; C069766:necdin