title: Most MCF7 and SK-OV3 cells were deprived of their stem nature by Hoechst 33342.
abstract: Using clonal and population analysis, we found that the MCF7 and SK-OV3 were composed mainly of cancer stem cells. Together with SP analysis, we found that both SP and NSP cells of MCF7 and SK-OV3 contained different proportions of cancer stem cells. In addition, we discovered that MCF7 SP cells were able to generate non-SP cells, and conversely non-SP cells generated SP cells; Moreover, we showed that Hoechst 33342 and FACS were harmful to the clonogenicity of MCF7 and SK-OV3 cells.
authors.affiliation: Department of Immunology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Pan Jia Yuan Nan Li, Chao Yang District, Beijing 100021, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhong
authors.forename: Chunxia
authors.initials: C
authors.lastname: Zhou
authors.forename: Wenbo
authors.initials: W
authors.lastname: Ma
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wang
authors.forename: Sujuan
authors.initials: S
authors.lastname: Guo
authors.forename: Xiaosan
authors.initials: X
authors.lastname: Su
authors.forename: Shuren
authors.initials: S
authors.lastname: Zhang
mesh_terms: D001562:Benzimidazoles / Q000494:pharmacology*; D045744:Cell Line, Tumor; D019070:Cell Lineage; D002469:Cell Separation; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006801:Humans; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000187:drug effects*
chemical_list: D001562:Benzimidazoles; D005456:Fluorescent Dyes; C017807:bisbenzimide ethoxide trihydrochloride