[Simultaneous determination of residues of three fluoroquinolones in chicken using high performance liquid chromatography with post-column derivatization and terbium sensitized fluorescence].
A method of high performance liquid chromatography with post-column derivatization and terbium sensitized fluorescence for simultaneous determination of residues of ciprofloxacin, norfloxacin and enrofloxacin in chicken was established, based on the effect that Tb3+ can sensibilize fluorescence of fluoroquinolones. Under the optimal chromatographic condition, three fluoroquinolones were separated on a C18 column with 0.05 mol/L acetic acid/acetate buffer-acetonitrile (89:11, v/v) as the mobile phase, and at a flow rate of 0.8 mL/min; then were derivatized with 8 x 10(-5) mol/L Tb3+ at 40 degrees C, and at a flow rate of 0.5 mL/min; finally were detected using a fluorescence detector (lamda(ex) = 271 nm, lamda(em) = 545 nm). The recoveries of three fluoroquinolones ranged from 66.3%-88.0% at the added levels of 1.0, 10.0, 50.0, and 100.0 ng/g, and the relative standard deviations (RSDs) were less than 15.0%. The linear range for quantitative analysis was between 0.1 and 500 ng/mL. All the RSDs of the inter-day and intra-day analyses were less than 13.0%. The detection limits were 0.05 ng/g for ciprofloxacin and norfloxacin and 0.08 ng/g for enrofloxacin. The method is sensitive for the determination of multi-residues of fluoroquinolones in chicken.
Qi
Kezong
K
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China. jwccqkz@ahau.edu.cn
Zhu
Liangqiang
L
Sun
Guoren
G
Shi
Zuhao
Z
Peng
Kaisong
K
D000818:Animals; D002645:Chickens / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000032:analysis; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000032:analysis*; D009643:Norfloxacin / Q000032:analysis; D015203:Reproducibility of Results; D013725:Terbium / Q000737:chemistry*
D024841:Fluoroquinolones; D013725:Terbium; D000077422:Enrofloxacin; D002939:Ciprofloxacin; D009643:Norfloxacin