title: [Contribution of indicators of sensitized T lymphocytes in the diagnosis of IgE independent and IgE dependent cow's allergy in children].
abstract: BACKGROUND
  Food allergy is an abnormal response of the immunological system, especially of mucosal immunological system on antigens supplied per os. There are very complicated and still unexplained immunological mechanisms, which lead to hypersensitivity reaction. Most often food hypersensitivity is identified as the effect of atopy, which is connected with humoral response (specific IgE antibody). On the other hand cell immunological response are less investigated, however they can be very important, especially as a significant factor to initiate pathological allergic processes.
  
  
  OBJECTIVE
  To investigate, the usefulness of flow cytometry for estimation of specific sensitization of subpopulation of lymphocytes on food allergens in the allergy diagnosis.
  
  
  METHODS
  The investigations were performed on 60 children from 6 months to 5 years old: 20 children with CM A IgE dependent, 20 with CM A IgE independent and 20 healthy children. IgE total, sIgE, IgG, IgA, IgM, basic immunological panel, CD 23, CD25, CD26, CD30, CD69, PCNA were measured.
  
  
  RESULTS
  We noticed decrease of expression of CD4+CD30+ between I and II examination (p=0.029), between I and III (p=0.009); decrease of expression of CD8+CD26+ between I and III test (p=0.038); decrease of expression of CD19+CD23+ between I and II examination (p=0.012) in I type of hypersensitivity. We observed a decrease of expression of CD4+CD25+ between I and III examine (p=0.026) and decrease of expression of CD4+ CD26+ between I and III examination (p=0.036) in IV type of hypersensitivity. Expression of CD69 was decreased after diet in IgE dependent allergy. Values of expression of PCNA are similar in I and IV type of hypersensitivity in children with CM A. Decrease of expression of PCNA in II examination was observed in both cases. Reintroduced allergen caused increase of expression of PCNA in both types of allergy (p=0.048 and p=0.041).
  
  
  CONCLUSIONS
  Our recent research confirms changes of the expression of T lymphocytes activation markers. It is connected with in vivo stimulation to allergen or with allergen elimination. The study of expression of activation markers using flow cytometry in food allergy in children can be helpful in observation of the dynamic progress process, but it cannot be used as a single diagnosis test.
authors.affiliation: Zakład Immunologii Klinicznej, Instytut Matki i Dziecka, ul. Kasprzaka 11a, 01-211 Warszawa, Poland. immunolog@imid.med.pl
authors.forename: Maria
authors.initials: M
authors.lastname: Gołebiowska-Wawrzyniak
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Markiewicz
authors.forename: Agata
authors.initials: A
authors.lastname: Kozar
authors.forename: Marta
authors.initials: M
authors.lastname: Pojawa
authors.forename: Jolanta
authors.initials: J
authors.lastname: Wacławek
authors.forename: Krystyna
authors.initials: K
authors.lastname: Jastrzebska-Janas
authors.forename: Iwona
authors.initials: I
authors.lastname: Czerwińska-Kartowicz
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Rytwiński
authors.forename: Piotr
authors.initials: P
authors.lastname: Derentowicz
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Matuszewska
authors.forename: Anna
authors.initials: A
authors.lastname: Stasiak-Barmuta
authors.forename: Jolanta
authors.initials: J
authors.lastname: Wasilewska
authors.forename: Urszula
authors.initials: U
authors.lastname: Daniluk
authors.forename: Zbigniew M
authors.initials: ZM
authors.lastname: Wawrzyniak
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology*; D015415:Biomarkers; D016022:Case-Control Studies; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007223:Infant; D008297:Male; D008892:Milk / Q000009:adverse effects* / Q000276:immunology; D016269:Milk Hypersensitivity / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D007073:Immunoglobulin E