[Determination of citrinin in red kojic by HPCE].
OBJECTIVE
To establish an instant determination method of citrinin in red kojic by high performance capillary electrophorphores for the first time.


METHODS
Red kojic was extracted with the mixtrue of Toluene and ethyl acetate (70:30). Separation was carried out in an uncoated fused silica capillary (50 microm x 45.0 cm). Meanwhile, a running voltage 15 kV, 20 mmol L(-1) borax buffer with 10.0 mmol L(-1) sodium deoxycholate (pH 9.3) and a UV detector at 212 nm were adopted.


RESULTS
Regression equation of citrinin was Y=9434 + 16781X (r =0.990), The lower limit of quantification (S/N > or =3) was 0.5 mg mL(-1). The assay coefficients of variation ranged from 98.8% to 101.1%. The intra and inter recovery ranged from 0.83 to 1.54% and from 1.86 to 5.09%. Twenty samples were determined with the method.


CONCLUSIONS
The method is proved to be simple, rapid and accurate, and it can be used to determine citrinin in red kojic.
Chen
Jun
J
State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burns, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. chenjpro@mail.tmmu.com.cn
Li
Zhang-wan
ZW
Qi
Wei
W
Liu
Zhong-rong
ZR
Li
Bo-gang
BG
D002953:Citrinin / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D032741:Monascus / Q000737:chemistry*; D015203:Reproducibility of Results
D002953:Citrinin