title: Complete nucleotide sequence of a putative new cytorhabdovirus infecting lettuce.
abstract: The full-length nucleotide sequence of the genomic RNA of a new cytorhabdovirus infecting lettuce was determined. Six open reading frames were found in the antigenomic sequence of the 12,926-nt negative-sense viral RNA genome. The genomic organisation was similar to that of lettuce necrotic yellows virus (LNYV), the type member of the genus Cytorhabdovirus: 3'-N-P-3-M-G-L-5', where N is the capsid protein gene, P the putative phosphoprotein gene, 3 a gene coding for a putative protein of unknown function, M the putative matrix protein gene, G the glycoprotein gene, and L the putative polymerase gene. Amino acid sequence comparison with the corresponding sequences of other rhabdoviruses revealed the closest relationship to LNYV, with identities ranging from 41% for the matrix proteins and 65% for the L polymerase proteins. These results indicate that this virus may be a member of a new cytorhabdovirus species, for which the name Lettuce yellow mottle virus (LYMoV) is proposed.
authors.affiliation: RLP Agroscience, AlPlanta - Institute for Plant Research, Neustadt an der Weinstrasse, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Heim
authors.forename: H
authors.initials: H
authors.lastname: Lot
authors.forename: B
authors.initials: B
authors.lastname: Delecolle
authors.forename: A
authors.initials: A
authors.lastname: Bassler
authors.forename: G
authors.initials: G
authors.lastname: Krczal
authors.forename: T
authors.initials: T
authors.lastname: Wetzel
mesh_terms: D001483:Base Sequence; D005814:Genes, Viral; D016679:Genome, Viral; D018545:Lactuca / Q000821:virology*; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics; D010942:Plant Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D012209:Rhabdoviridae / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014763:Viral Matrix Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014763:Viral Matrix Proteins; D014764:Viral Proteins