title: Comparison of different DNA extraction and polymerase chain reaction methods to detect Leptospira spp. on field samples.
abstract: It was carried out a comparison on two reference Leptospira strains DNA between different extraction methods and two polymerase chain reaction protocol. The DNA was quantified and serial dilutions were tested by polymerase chain reaction. The results showed difference in terms of recovery and sensitivity between this methods.
authors.affiliation: Istituto Zooprofilatico Sperimentale della Sicilia A. Mirri, Palermo, Italy. mvitale@pa.izs.it
authors.forename: Maria
authors.initials: M
authors.lastname: Vitale
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Vitale
authors.forename: Stefano
authors.initials: S
authors.lastname: Reale
authors.forename: Gesualdo
authors.initials: G
authors.lastname: Vesco
authors.forename: Vittoria
authors.initials: V
authors.lastname: Currò
authors.forename: Santo
authors.initials: S
authors.lastname: Caracappa
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D007558:Italy; D007919:Leptospira / Q000235:genetics* / Q000302:isolation & purification; D041401:Leptospira interrogans serovar pomona / Q000235:genetics / Q000302:isolation & purification; D007922:Leptospirosis / Q000235:genetics / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial