Alterations in glutathione reductase, superoxide dismutase, and lipid peroxidation of tadpoles (Xenopus laevis) exposed to Bonny Light crude oil and its fractions.
We determined the effects of sub-lethal levels of Bonny Light crude oil (WC) and its water soluble (WSF) and insoluble (WIF) fractions on malondialdehyde (MDA) and stress enzymes in tadpoles (Xenopus laevis) following two and 4 weeks exposure at different concentrations. We observed that the treatment of tadpoles with WC, WSF, or WIF decreased the weight of tadpoles, increased MDA levels, and increased superoxide dismutase (SOD) and glutathione reductase (GR) at lower concentrations of exposure and decreased the enzymes at higher doses of exposure. We found that the WC had a lesser negative effect on the parameters of tadpoles compared to the WSF. Longer exposure of tadpoles to WC, WSF, or WIF even at lower concentrations resulted in a negative effect on MDA, SOD, and GR activities The study shows that sub-lethal contaminations with WC, WSF, or WIF induces membrane lipid peroxidation and reduce the ability of tadpoles to produce SOD and GR which may have metabolic costs.
Eriyamremu
G E
GE
Department of Biochemistry, University of Benin, PMB 1154, Benin City, Nigeria. georgeeriyamremu@yahoo.com
Osagie
V E
VE
Omoregie
S E
SE
Omofoma
C O
CO
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005980:Glutathione Reductase / Q000378:metabolism*; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D019216:Metals, Heavy / Q000737:chemistry; D010578:Petroleum / Q000032:analysis / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism*; D014867:Water / Q000737:chemistry; D015430:Weight Gain / Q000187:drug effects; D014982:Xenopus laevis / Q000254:growth & development / Q000378:metabolism*
D019216:Metals, Heavy; D010578:Petroleum; D014867:Water; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005980:Glutathione Reductase