Identification of the domains required for the localization of Prp19p to lipid droplets or the nucleus.
Prp19p is a protein found in the nucleus, cytosol or lipid droplets depending on the cell type. Prp19p participates in pre-mRNA splicing, in neuronal/astroglial cell fate decisions or in adipocyte lipid droplet biogenesis. In this study, the motifs of Prp19p that are necessary for its localization to lipid droplets or the nucleus in 3T3-L1 adipocytes are investigated using a series of truncated mutants of Prp19p that were fused to EGFP and transiently introduced into differentiated 3T3-L1 adipocytes. Immunofluorescence microscopy revealed that a domain of amino acids 167-250 is necessary for the recruitment of Prp19p to lipid droplets and that a domain of amino acids 1-166 is necessary for the recruitment of Prp19p to a nucleus.
Cho
Si Young
SY
R & D Center, AmorePacific Corporation, 314-1 Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-729, Republic of Korea.
Park
Pil Joon
PJ
Lee
Jeong Ho
JH
Kim
Jung Joo
JJ
Lee
Tae Ryong
TR
D041721:3T3-L1 Cells; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D050356:Lipid Metabolism / Q000502:physiology*; D051379:Mice; D034521:Nuclear Matrix-Associated Proteins / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000502:physiology; D000072260:RNA Splicing Factors; D012996:Solutions; D013329:Structure-Activity Relationship
D034521:Nuclear Matrix-Associated Proteins; C518638:Prpf19 protein, mouse; D000072260:RNA Splicing Factors; D012996:Solutions