Measurement of lipid forces by X-ray diffraction and osmotic stress.
Lipid suspensions in aqueous solutions most often form multilamellar vesicles of uniformly spaced bilayers. Interlamellar spacing is determined by the balance of attractive van der Waals (charge fluctuation) and repulsive forces. This balance of forces, as well as membrane elasticity, can be probed by applied osmotic stress. We describe how osmotic stress can be imposed on multilamellar lipid samples to study lipid interactions.
Petrache
Horia I
HI
Department of Physics, Indiana University, Indianapolis, USA.
Harries
Daniel
D
Parsegian
V Adrian
VA
D004548:Elasticity; D008051:Lipid Bilayers / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D009997:Osmotic Pressure; D014961:X-Ray Diffraction
D008051:Lipid Bilayers; D008055:Lipids