Methods to study the interactions of the dynein light chains and intermediate chains.
The cargo-binding domain of the cytoplasmic dynein complex consists of an intermediate chain, a light-intermediate chain, and three families of light chains. These five subunits form the base of the dynein complex. Variations in the composition and interactions of these subunits play an important role for selecting a particular cargo and regulating dynein function. By using several complementary binding methods, we have investigated the protein-protein interaction in the cargo-binding domain of the cytoplasmic dynein.
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Kevin W-H
KW
Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA, USA.
Pfister
K Kevin
KK
D001665:Binding Sites; D001671:Biochemistry / Q000379:methods*; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism; D029721:Drosophila Proteins / Q000737:chemistry* / Q000378:metabolism; D004398:Dyneins / Q000737:chemistry; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D008869:Microtubule-Associated Proteins / Q000737:chemistry; D008870:Microtubules / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D025941:Protein Interaction Mapping
D002352:Carrier Proteins; D029721:Drosophila Proteins; D008869:Microtubule-Associated Proteins; D005982:Glutathione Transferase; D004398:Dyneins