Microbial manipulation of the rat dam changes bacterial colonization and alters properties of the gut in her offspring.
The impact of an altered bacterial colonization on gut development has not been thoroughly studied, despite the increased risk of certain diseases with a disturbed microbiota after birth. This study was conducted to determine the effect of microbial manipulation, i.e., antibiotic treatment or Escherichia coli exposure, of the dam on bacterial colonization and gut development in the offspring. Pregnant rats were administered either broad-spectrum antibiotics 3 days before parturition or live nonpathogenic E. coli Culture Collection of University of Göteborg, Sweden type strain (CCUG 29300(T)) 1 wk before parturition and up to 14 days of lactation in the drinking water. Cecal bacterial levels, gut growth, intestinal permeability, digestive enzyme levels, and intestinal inflammation were studied in 2-wk-old rats. Pups from dams that were antibiotic-treated had higher densities of Enterobacteriaceae, which correlated with a decreased stomach growth and function, lower pancreatic protein levels, higher intestinal permeability, and increased plasma levels of the acute phase protein, haptoglobin, compared with pups from untreated mothers. Exposure of pregnant/lactating mothers to E. coli CCUG 29300(T), also resulting in increased Enterobacteriaceae levels, gave in the offspring similar results on the stomach and an increased small intestinal growth compared with the control pups. Furthermore, E. coli pups showed increased mucosal disaccharidase activities, increased liver, spleen, and adrenal weights, as well as increased plasma concentrations of haptoglobin. These findings indicate that disturbing the normal bacterial colonization after birth, by increasing the densities of cecal Enterobacteriaceae, appears to have lasting effects on the postnatal microflora, which affects gut growth and function.
Fåk
Frida
F
Department of Cell and Organism Biology, Lund Univ., Helgonavägen 3B, SE-223 62 Lund, Sweden. Frida.Fak@cob.lu.se
Ahrné
Siv
S
Molin
Göran
G
Jeppsson
Bengt
B
Weström
Björn
B
D000818:Animals; D000831:Animals, Newborn; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002432:Cecum / Q000187:drug effects / Q000382:microbiology; D004186:Disaccharidases / Q000378:metabolism; D004755:Enterobacteriaceae / Q000187:drug effects / Q000254:growth & development*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005260:Female; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D006242:Haptoglobins / Q000378:metabolism; D007421:Intestine, Small / Q000187:drug effects / Q000382:microbiology; D007774:Lactation; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development*; D008099:Liver / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology; D010539:Permeability; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000187:drug effects; D013270:Stomach / Q000187:drug effects / Q000382:microbiology; D013950:Thymus Gland / Q000187:drug effects; D013997:Time Factors
D000900:Anti-Bacterial Agents; D006242:Haptoglobins; D004186:Disaccharidases