title: Membrane protein insertion and secretion in bacteria.
abstract: Export of secretory proteins across and insertion of membrane proteins into the cytoplasmic membrane of Escherichia coli and other bacteria is mediated by the enzyme complex translocase. The last decade has seen a major advance in the understanding of the mechanism of these processes. A large part of this progress can be attributed to the development of general and powerful methods to study the translocase activity in vitro. Here we describe a transcription-translation method used to analyze the insertion of membrane proteins into E. coli inner membrane vesicles and a rapid and quantitative fluorescent method to analyze the translocation of secretory proteins.
authors.affiliation: Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Materials Science Center Plus, University of Groningen, The Netherlands.
authors.forename: Jeanine
authors.initials: J
authors.lastname: de Keyzer
authors.forename: Martin
authors.initials: M
authors.lastname: van der Laan
authors.forename: Arnold J M
authors.initials: AJ
authors.lastname: Driessen
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008954:Models, Biological; D011499:Protein Processing, Post-Translational; D021381:Protein Transport
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins