[Relationship of human leucocyte antigen G, E with intrahepatic cholestasis of pregnancy].
OBJECTIVE
To investigate the relationship between human leucocyte antigen (HLA)-G, E and intrahepatic cholestasis of pregnancy (ICP).


METHODS
In situ hybridization and immunohistochemistry methods were adopted in this study to detect the expression of HLA-G and HLA-E mRNA and protein in the placentae of three groups, namely normal control group, ICP group with dexamethasone (DEX) treatment, and ICP group without DEX treatment.


RESULTS
No significant differences in respect to HLA-G, HLA-E mRNA expression on extrovillous cytotrophoblasts (EVCT ) of the placentae were found between the normal control group, the ICP group with DEX treatment and the ICP group without DEX treatment. However, the protein expression of HLA-G and HLA-E on the placental EVCT of ICP group without DEX treatment was significantly lower than that of the normal control group and the ICP group with DEX treatment.


CONCLUSIONS
The decreased EVCT expression of HLA-G, HLA-E protein may cause the disturbance of the maternal-fetal immune tolerance of ICP patient and play a role in the pathogenesis of ICP. DEX can upregulate the EVCT expression of HLA-G, HLA-E protein; this may be one of the underlying mechanisms for its use in the treatment of ICP.
Peng
Bing
B
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China. pengbin-a111@163.com
Liu
Shu-yun
SY
Wang
Xiao-dong
XD
Wang
He
H
Zhang
Li
L
Hu
Min
M
D016022:Case-Control Studies; D002780:Cholestasis, Intrahepatic / Q000188:drug therapy / Q000235:genetics* / Q000276:immunology; D003907:Dexamethasone; D005260:Female; D006680:HLA Antigens / Q000235:genetics* / Q000276:immunology; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology; D006801:Humans; D010920:Placenta / Q000276:immunology / Q000378:metabolism; D011247:Pregnancy; D011248:Pregnancy Complications / Q000235:genetics* / Q000276:immunology; D000097625:HLA-E Antigens
D003907:Dexamethasone; D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D000097625:HLA-E Antigens