Lipase-catalyzed selective synthesis of monolauroyl maltose using continuous stirred tank reactor.
Monolauroyl maltose was synthesized by an immobilized lipase that catalyzed condensation of maltose and lauric acid in acetone using a batch reactor or a continuous stirred tank reactor. Mono- and di-lauroyl maltoses were identified by FT-IR, (1)H NMR, (13)C NMR and MS. Monolauroyl maltose was selectively synthesized in a continuous stirred tank reactor and no diester was detected. The highest concentration of monolauroyl maltose at 28 mmol/l was obtained in 250 ml acetone when maltose was added at 4 g/d and the molar ratio of lauric acid to maltose was fixed at 4:1 at a flow rate of 0.15 ml/min for both influx and effluent without supplement of fresh molecular sieve.
Liu
Qiaoyu
Q
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China.
Jia
Chengsheng
C
Kim
Jin M
JM
Jiang
Pingping
P
Zhang
Xiaoming
X
Feng
Biao
B
Xu
Shiying
S
D000096:Acetone / Q000378:metabolism; D019149:Bioreactors; D002175:Candida / Q000201:enzymology*; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D007700:Kinetics; D007848:Laurates / Q000378:metabolism*; D007850:Lauric Acids / Q000378:metabolism; D008049:Lipase / Q000378:metabolism*; D008320:Maltose / Q000096:biosynthesis* / Q000302:isolation & purification
D004800:Enzymes, Immobilized; D007848:Laurates; D007850:Lauric Acids; C030358:lauric acid; D000096:Acetone; D008320:Maltose; D008049:Lipase