title: Targeted migration and differentiation of engrafted neural precursor cells in amyloid beta-treated hippocampus in rats.
abstract: OBJECTIVE
  To observe the migration and differentiation of the neural precursor cells (NPCs) that derived from murine embryonic stem cells (ESCs) when they were transplanted into amyloid beta (A beta)-treated rat hippocampus.
  
  
  METHODS
  MESPU35, a murine ESC cell line that express the enhanced green fluorescent protein (EGFP), was induced differentiation into nestin-positive NPCs by modified serum-free methods. The A beta plaques and the differentiation of the grafted cells were observed by immunofluorescent staining.
  
  
  RESULTS
  Comparing 16 weeks with 4 weeks post-transplantation, the migration distance increased about 5 times; the rate of migratory NPCs differentiating into glial fibrillary acidic protein (GFAP)-positive cells kept rising from (30.41+/-1.45) % to (49.25+/-1.23) %, and the rate of NPCs differentiating into neurofilament 200 (NF200) positive cells increased from (16.68+/-0.95) % to (27.94+/-1.21) %. Meanwhile, the GFAP-positive cells targeting to the ipsilateral side of A beta plaques increased from 60.2% to 81.3%, while the NF200-positive cells increased from 61.3% to 84.1%. The migration distance had significant positive linear correlations to the neuronal differentiation rate (r = 0.991) and to the astrocytic differentiation rate (r = 0.953).
  
  
  CONCLUSIONS
  Engrafted NPCs migrate targetedly to the A beta injection site and differentiate into neurons and astrocytes.
authors.affiliation: Department of Physiology, Department of Neurobiology, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.forename: Hai-Wei
authors.initials: HW
authors.lastname: Xu
authors.forename: Xiao-Tang
authors.initials: XT
authors.lastname: Fan
authors.forename: Zhi-Fang
authors.initials: ZF
authors.lastname: Li
authors.forename: Da-Bing
authors.initials: DB
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Guang-Ji
authors.initials: GJ
authors.lastname: Zhou
mesh_terms: D016229:Amyloid beta-Peptides / Q000008:administration & dosage / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D002465:Cell Movement; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology* / Q000502:physiology; D007276:Injections, Intraventricular; D008297:Male; D009474:Neurons / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D051381:Rats; D017208:Rats, Wistar; D033581:Stem Cell Transplantation
chemical_list: D016229:Amyloid beta-Peptides; D005904:Glial Fibrillary Acidic Protein; D049452:Green Fluorescent Proteins