Colonic healing after portal ischemia and reperfusion: an experimental study with oxidative stress biomarkers.
This experimental study aimed to evaluate colon healing after portal ischemia followed by reperfusion. Seventy male Wistar rats randomly distributed in four groups were used: Group 1, colonic anastomosis (n = 20); Group 2, portal ischemia-reperfusion (n = 20); Group 3, colonic anastomosis and portal ischemia-reperfusion (n = 20); and Group 4, control (n = 10). In the postoperative period, these rats were re-allocated into subgroups and lipid peroxidation and protein oxidation plasma levels were evaluated on days 1 and 5 by thiobarbituric acid reactive substances (TBARS) and slot-blotting assays, respectively. A segment of the right colon was also removed for collagen analysis. Both malondialdehyde (MDA) and protein carbonyl levels (oxidative markers of lipids and proteins) presented a significant increase after reperfusion in Group 3 on days 1 (P < 0.002) and 5 (P < 0.0001). In this same group, an extensive inflammatory process showing decreased fibroplasia was observed, with deficiency in collagen deposition on both sides of the anastomosis edges. Taken together, these results indicate that portal congestion followed by reperfusion induces an oxidative stress, which impaired the mechanism of colon anastomotic healing.
Rolim
Marcia Farias
MF
Experimental Surgical Center of the Department of Surgery, Faculty of Medicine, Federal University of Rio de Janeiro, and FAPERJ, Rio de Janeiro, Brazil.
Riger
Cristiano J
CJ
Eleutherio
Elis C A
EC
Colão
Cristiane da Fonseca
Cda F
Pereira
Gerson Cotta
GC
Schanaider
Alberto
A
D000714:Anastomosis, Surgical; D000818:Animals; D002244:Carbon / Q000737:chemistry; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D003106:Colon / Q000473:pathology*; D007150:Immunohistochemistry; D015227:Lipid Peroxidation; D008055:Lipids / Q000737:chemistry; D008297:Male; D008315:Malondialdehyde / Q000737:chemistry; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury; D017392:Thiobarbituric Acid Reactive Substances
D008055:Lipids; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D002244:Carbon; D003094:Collagen; D010100:Oxygen