title: Development of HPLC and NACE methods for the simultaneous determination of benzoic and sorbic acids in sour snap beans containing oil.
abstract: The practical methods were developed for the simultaneous determination of benzoic acid (BA) and sorbic acid (SA) in sour snap bean samples containing oil. BA and SA in the samples were extracted by ultrasonication with water, followed by cleanup procedures with precipitation for removing the potential proteins and with petroleum ether liquid-liquid extraction for removing the edible oil contained in the samples. The HPLC method was developed using Supelco C18 (250 mm x 4.6 mm id, 5 microm) as column, MeOH-20 mM NH(4)Ac (25:75 v/v) at 1.0 mL/min as the mobile phase and 230 nm as the detection wavelength. The optimal NACE method was established with a running buffer of 20.0 mM NH(4)Ac in 95% MeOH (pH* 10.6), and an applied voltage of -30 kV over a capillary of 50 microm id x 48.5 cm (40 cm to the detector window), which gave a baseline separation of BA and SA, and as well as of the blank matrix within ca. 10 min. Both HPLC and NACE methods gave the relatively lower limits of quantification at about 0.01-0.02 and 0.04-0.05 mg/kg, respectively, whereas the overall recoveries were larger than 85.0%. The proposed methods have been successfully applied to measure 15 real sour bean samples and the content profile of BA and SA in sour bean samples was obtained and evaluated.
authors.affiliation: Chemistry Department, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou, P R China.
authors.forename: Po
authors.initials: P
authors.lastname: Han
authors.forename: Zhenmin
authors.initials: Z
authors.lastname: Jia
authors.forename: Mian
authors.initials: M
authors.lastname: Liu
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Li
authors.forename: Hongxia
authors.initials: H
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.forename: Xie
authors.initials: X
authors.lastname: Wang
authors.forename: Fuguo
authors.initials: F
authors.lastname: Ban
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
mesh_terms: D019817:Benzoic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D007887:Fabaceae / Q000737:chemistry*; D008722:Methods; D010938:Plant Oils; D013011:Sorbic Acid / Q000032:analysis*
chemical_list: D010938:Plant Oils; D019817:Benzoic Acid; D013011:Sorbic Acid