A BAC clone fingerprinting approach to the detection of human genome rearrangements.
We present a method, called fingerprint profiling (FPP), that uses restriction digest fingerprints of bacterial artificial chromosome clones to detect and classify rearrangements in the human genome. The approach uses alignment of experimental fingerprint patterns to in silico digests of the sequence assembly and is capable of detecting micro-deletions (1-5 kb) and balanced rearrangements. Our method has compelling potential for use as a whole-genome method for the identification and characterization of human genome rearrangements.
Krzywinski
Martin
M
BC Cancer Agency Genome Sciences Centre, West 7th Avenue, Vancouver, British Columbia, Canada V5Z 4S6. martink@bcgsc.ca
Bosdet
Ian
I
Mathewson
Carrie
C
Wye
Natasja
N
Brebner
Jay
J
Chiu
Readman
R
Corbett
Richard
R
Field
Matthew
M
Lee
Darlene
D
Pugh
Trevor
T
Volik
Stas
S
Siddiqui
Asim
A
Jones
Steven
S
Schein
Jacquie
J
Collins
Collin
C
Marra
Marco
M
D022202:Chromosomes, Artificial, Bacterial; D003198:Computer Simulation; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004262:DNA Restriction Enzymes; D015321:Gene Rearrangement / Q000235:genetics*; D015894:Genome, Human / Q000235:genetics*; D006801:Humans
D017931:DNA Primers; D004262:DNA Restriction Enzymes