title: Selective one-step extraction of Arabidopsis thaliana seed oleosins using organic solvents.
abstract: Oleosins are hydrophobic proteins from oleaginous seeds, surrounding and stabilizing oil bodies. They are known to display interesting interfacial properties. Specific sera were raised against four different A. thaliana oleosins and used in dot-blot assays for oleosin quantification. These assays were used to set up extraction of oleosins from A. thaliana seeds. One mixture of chloroform/methanol gave optimal oleosin extraction. Extracted proteins represented 9% of seed proteins and were identified by immunoblot and proteomic analyses. Oleosins accounted for 79% of the extracted proteins. This simple one-step procedure allows selective extraction and concentration of oleosins from seeds without tedious oil body purification. Oleosin extract was indeed used to demonstrate the presence of the rare oleosin S5 in mature seeds. Moreover, this method will be useful to investigate the potential use of oleosins as emulsifier and to question their possible allergenicity.
authors.affiliation: UMR 206 Chimie Biologique, AgroParisTech, INRA, F-78850 Thiverval-Grignon, France. andrea@grignon.inra.fr
authors.forename: Sabine
authors.initials: S
authors.lastname: D'Andréa
authors.forename: Pascale
authors.initials: P
authors.lastname: Jolivet
authors.forename: Céline
authors.initials: C
authors.lastname: Boulard
authors.forename: Colette
authors.initials: C
authors.lastname: Larré
authors.forename: Marine
authors.initials: M
authors.lastname: Froissard
authors.forename: Thierry
authors.initials: T
authors.lastname: Chardot
mesh_terms: D017360:Arabidopsis / Q000737:chemistry*; D029681:Arabidopsis Proteins / Q000032:analysis* / Q000737:chemistry*; D002725:Chloroform / Q000737:chemistry; D015151:Immunoblotting; D000432:Methanol / Q000737:chemistry; D010938:Plant Oils / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D012995:Solubility
chemical_list: D029681:Arabidopsis Proteins; D010938:Plant Oils; C079353:oleosin protein, Arabidopsis; D002725:Chloroform; D000432:Methanol