[Characteristic analysis on the toxin of microcystis in life water source of Zhengzhou City].
OBJECTIVE
To analyze the toxic characteristic of microcystis in a life water source.


METHODS
Microcystis in Huayuankou conservation pool and Xiliu lake were isolated by 96-microbiological assay and supreme dilution methods. Phycocyanin intergenic spacer region (PC-IGS) and microcystin synthetase gene B (McyB) were detected by whole-cell PCR method, And toxicities of microcystis were identified by solid pole of extraction (SPE) and high performance liquid chromatography (HPLC) method.


RESULTS
The microcystis of the water source were cyanobacteria which could contain PC-IGS and McyB expressing positively, Comparition with reported meyB of Microcystis aeruginosa in Genbank, the homology of gene sequence was 99%. The main toxic isomers of the three microcystis were MC-LR, whose proportions of the whole toxin were 97.9%, 98.6% and 99.3%, respectively.


CONCLUSIONS
The microcystis of the main life water source maybe poisonous and the main isomer maybe MC-LR.
Yang
Songqin
S
Department of Environmental Toxicology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Ba
Yue
Y
Zhang
Huizhen
H
Cheng
Xuemin
X
D001426:Bacterial Proteins / Q000235:genetics; D002681:China; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis / Q000302:isolation & purification*; D046931:Microcystis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010453:Peptide Synthases / Q000235:genetics; D010798:Phycocyanin / Q000235:genetics; D014871:Water Microbiology; D014873:Water Pollutants / Q000032:analysis*; D014881:Water Supply / Q000032:analysis*
D001426:Bacterial Proteins; D008387:Marine Toxins; D052998:Microcystins; D014873:Water Pollutants; D010798:Phycocyanin; D010453:Peptide Synthases; C432283:microcystin synthetase A; C057862:cyanoginosin LR