title: Separation of murine neutrophils and macrophages by thermoresponsive magnetic nanoparticles.
abstract: Magnetic particles have been used widely in both biotechnological and medical fields, including for immunoassay, enzyme immobilization, drug transport, and immunological diagnosis. Especially particles with bioactive molecules such as antibodies and streptavidin are very useful tools for cell separation. Here we report affinity selection of neutrophils and macrophages from peritoneal inflammatory cells performed by thermoresponsive magnetic nanoparticles conjugated with macrophage-specific anti-F4/80 antibody. The magnetic nanoparticles, which are capped with thermoresponsive polymers, are aggregated by heating the particles over 30 degrees C and show their intrinsic magnetism. The neutrophils are concentrated approximately 90% by these magnetic nanoparticles without any activation, indicating that this novel cell separation method could fulfill a wide range of applications in analysis of the isolation of fragile cells such as neutrophils.
authors.affiliation: International Clinical Research Center, Research Institute, International Medical Center of Japan, Tokyo, Japan.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Hoshino
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Ohnishi
authors.forename: Masato
authors.initials: M
authors.lastname: Yasuhara
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D018189:Immunomagnetic Separation / Q000379:methods*; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry*; D009504:Neutrophils / Q000166:cytology*; D013696:Temperature