[Time-resolved fluoroimmunoassay for detecting hepatitis B virus surface antigen].
A convenient, highly sensitive and highly specific method using time-resolved fluoro immunoassay (TRFIA) for quantitative detection of hepatitis B virus is described. Using EU-DTTA as the tracer molecule, the assay showed a detection range of the standard curves of 0.2-300 ng/ml for HbsAg with a sensitivity of 0.05 ng/ml. The within-run coefficient of variations for standard samples were less than 10%, and the correlation coefficient between radioimmunoassay and TRFIA assay results reached 95% for the same sample, demonstrating the advantages of TRFIA for its wide detection range, high sensitivity and simple operation and its great potentials for clinical use.
Lin
De-Qiu
DQ
College of Life Sciences, South China Normal University, Guangzhou 510631, China.
Shen
Jian
J
D015200:Fluoroimmunoassay / Q000379:methods*; D006509:Hepatitis B / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006801:Humans; D012680:Sensitivity and Specificity
D006514:Hepatitis B Surface Antigens