Identification of strain-specific DNA of Actinobacillus actinomycetemcomitans by representational difference analysis.
A genomic subtraction method, the representational difference analysis (RDA), was tested for its use in identifying strain-specific DNA in Actinobacillus actinomycetemcomitans. Subtraction of strain D7S with strain HK1651 yielded D7S-specific 2.3-kilobase (kb) islet-A and 5.3-kb islet-B. Islet-A contains a 1.5-kb region that is homologous to a region found in the A. actinomycetemcomitans plasmid pVT745. Islet-B contains a 2.1-kb homolog of vgr, a component of a DNA repeat element rhs. The distribution of these islets among A. actinomycetemcomitans strains was further examined by polymerase chain reaction. Islet-A was found in nine serotype a and two serotype b strains but was missing from 34 strains. Islet-B was found in one serotype a strain, four serotype d strains and two serotype e strains, but was missing from 34 strains.
Mena
J
J
Division of Primary Oral Health Care, School of Dentistry, University of Southern California, Los Angeles, CA 90089, USA.
Chen
C
C
D016976:Aggregatibacter actinomycetemcomitans / Q000145:classification / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics; D044404:Genomic Islands / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D012703:Serotyping; D013045:Species Specificity
D001426:Bacterial Proteins; D004269:DNA, Bacterial