title: Sequencing and assembly of a full-length infectious clone of grapevine virus B and its infectivity on herbaceous plants.
abstract: Grapevine virus B (GVB) has been found associated with corky bark-diseased vines. Although the sequence of a 7.6-kb cDNA clone from a GVB isolate from Italy has been described, striking differences in sequences between GVB isolates prompted us to construct an additional full-length GVB clone from the isolate 94/971 and to determine its complete sequence. The cDNA of GVB 94/971 shared a nucleotide sequence identity of only 77% with the GVB isolate from Italy. The cDNA of GVB 94/971 was infectious on Nicotiana plants as demonstrated by symptoms and by means of Northern blot, Western blot and electron microscopic analyses.
authors.affiliation: The S. Tolkowsky Laboratory, Department of Virology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Moskovitz
authors.forename: D E
authors.initials: DE
authors.lastname: Goszczynski
authors.forename: L
authors.initials: L
authors.lastname: Bir
authors.forename: A
authors.initials: A
authors.lastname: Fingstein
authors.forename: H
authors.initials: H
authors.lastname: Czosnek
authors.forename: M
authors.initials: M
authors.lastname: Mawassi
mesh_terms: D018076:DNA, Complementary / Q000235:genetics; D052677:Flexiviridae / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D016679:Genome, Viral; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014026:Nicotiana / Q000821:virology
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral