[Simultaneous determination of geniposide, baicalin and berberine hydrochloride in Angong Niuhuang pill by RP-HPLC].
OBJECTIVE
A new method for the simultaneous quantitative determination of geniposide, baicalin and berberine hydrochloride in Angong Niuhuang pill using reversed phase high performance liquid chromatographic method had been developed.


METHODS
The optimum chromatographic conditions were as follows: Agilent Zorbax SB - C18 column (4.6 mm 250 mm, 5 m), acetonitrile-H2O (containing 6 mmol L(-1) KH2PO4, pH 4.6) gradient elution; as a detection wavelength of 343 nm.


RESULTS
The calibration curves of geniposide, baicalin and berberine were linear at the ranges of 4.50-110.00, 5.00-153.00, 6.40-191.00 mg L(-1), respectively. The limits of detection of the method were 0.77 ng for geniposide, 1.53 ng for baicalin and 1.43 ng for berberine hydrochloride. The recoveries of the method were 104.44% (RSD 1.79% ) for geniposide, 96.98% (RSD 1.76%) for baicalin, 101.08% (RSD 3.1%) for berberine hydrochloride.


CONCLUSIONS
This method had been successfully applied to determine the content of geniposide, baicalin and berberine hydrochloride in Angong Niuhuang pill.
Han
Feng-mei
FM
Hubei Province Key Laboratory of Biotechnology of Traditional Chinese Medicine, Hubei University, Wuhan 430062, China.
Li
Yu
Y
Li
Lu-jun
LJ
Chen
Yong
Y
D001599:Berberine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D005419:Flavonoids / Q000032:analysis*; D039823:Iridoids / Q000032:analysis*; D008419:Materia Medica / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011714:Pyrans / Q000032:analysis*
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D039823:Iridoids; D008419:Materia Medica; D011714:Pyrans; D001599:Berberine; C007835:geniposide; C038044:baicalin