In vitro efficacy of ribavirin against canine distemper virus.
Despite vaccination, canine distemper virus (CDV) remains one of the important pathogen of dogs with worldwide distribution. Ribavirin (RIB) inhibits replication of measles virus (MV), a morbillivirus closely related to CDV, both in vitro and in vivo. In this report the antiviral activity of RIB against CDV in cell cultures was assessed. Quantitative real-time RT-PCR was used to measure viral RNA in VERO cells infected by CDV and to evaluate the inhibitory effects of RIB. RIB caused a dose- and time-dependent decrease in accumulation of CDV RNA when added after virus adsorption. RIB was highly effective in preventing CDV replication at low concentrations with 50% virus-inhibitory concentrations ranging from 0.02 to 0.05 mM. Such low values were comparable to values displayed by highly susceptible strains of MV. In addition, CDV was passaged sequentially in VERO cell monolayers in the presence of RIB to trigger viral extinction. The virus was no longer detected after three passages, suggesting that error catastrophe is one of the modes of action of RIB against CDV. These findings suggest RIB as a promising tool for the therapy of CD in dogs.
Elia
Gabriella
G
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, 70010 Valenzano, Bari, Italy. g.elia@veterinaria.uniba.it
Belloli
Chiara
C
Cirone
Francesco
F
Lucente
Maria Stella
MS
Caruso
Marta
M
Martella
Vito
V
Decaro
Nicola
N
Buonavoglia
Canio
C
Ormas
Paolo
P
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D004217:Distemper Virus, Canine / Q000187:drug effects*; D004285:Dogs; D020128:Inhibitory Concentration 50; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000037:antagonists & inhibitors; D012254:Ribavirin / Q000494:pharmacology*; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D012367:RNA, Viral; D012254:Ribavirin