title: Distribution of enterotoxin genes among carriage- and infection-associated isolates of Staphylococcus aureus.
abstract: OBJECTIVE
  To compare the distribution of genes encoding classical and newly described enterotoxins among Staphylococcus aureus, associated with carriage and infection.
  
  
  RESULTS
  Forty-five nasal isolates from carriers and 42 clinical isolates were included. The genes sea to see and seg to sei as well as sem, sen, seo and seu were tested using multiplex and conventional PCR. The most frequently found toxin genes were egc-related genes, in particular the combination seg and sei (n = 55, 63.1%), followed by sen and seu (n = 54, 62.1%), sem (n = 51, 58.6%) and seo (n = 48, 55.2%). Significant differences were found for seg and sei combination (33 of the nasal vs 22 of the infection isolates, P = 0.048) as well as for the genes sem (P = 0.004), sen (P = 0.029) and seo (P = 0.032). Regarding the classical toxin genes no significant differences between the two groups of isolates were found.
  
  
  CONCLUSIONS
  Significant differences between infection and carriage strains were found only for the egc-related genes, which were more common in the nasal isolates.
  
  
  CONCLUSIONS
  The egc-related enterotoxin genes seem to be more prevalent in carriage- than in infection-associated S. aureus isolates. The possible contribution of egc-related genes in determining the potential for nasal carriage requires further investigation.
authors.affiliation: National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria. nashev@hotmail.com
authors.forename: D
authors.initials: D
authors.lastname: Nashev
authors.forename: K
authors.initials: K
authors.lastname: Toshkova
authors.forename: L
authors.initials: L
authors.lastname: Bizeva
authors.forename: O
authors.initials: O
authors.lastname: Akineden
authors.forename: C
authors.initials: C
authors.lastname: Lämmler
authors.forename: M
authors.initials: M
authors.lastname: Zschöck
mesh_terms: D002353:Carrier State / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D009666:Nose / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D004768:Enterotoxins