title: Isolation and identification of antiplatelet aggregatory principles from the leaves of Piper lolot.
abstract: The methanolic extract of Piper lolot, having shown potent inhibitory activity on platelet aggregation induced by arachidonic acid (AA) and platelet activating factor (PAF), was subjected to activity-guided isolation to yield twelve new amide alkaloids, piperlotine A-L (1-12), along with twenty-nine known compounds. Their structures were elucidated on the basis of spectroscopic analysis. The isolated compounds were tested for their inhibitory activity on the rabbit platelet aggregation. The compounds piperlotine A (1), piperlotine C (3), piperlotine D (4), piperlotine E (5), 3-phenyl-1-(2,4,6-trihydroxyphenyl)propan-1-one (21), 3-(4-methoxyphenyl)-1-(2,4,6-trihydroxyphenyl)propan-1-one (22), 1-trans-cinnamoylpyrrolidine (24), sarmentine (26), pellitorine (27), methyl 3-phenylpropionate (32), and (10S)-10-hydroxypheophorbide a methyl ester (40) showed potent antiplatelet aggregation activity.
authors.affiliation: Department of Chemistry, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chia-Ying
authors.initials: CY
authors.lastname: Li
authors.forename: Wei-Jern
authors.initials: WJ
authors.lastname: Tsai
authors.forename: Amooru Gangaiah
authors.initials: AG
authors.lastname: Damu
authors.forename: E-Jian
authors.initials: EJ
authors.lastname: Lee
authors.forename: Tian-Shung
authors.initials: TS
authors.lastname: Wu
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Dung
authors.forename: Tran Dinh
authors.initials: TD
authors.lastname: Thang
authors.forename: Le
authors.initials: L
authors.lastname: Thanh
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D031701:Piper / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D011817:Rabbits
chemical_list: D000470:Alkaloids; D010975:Platelet Aggregation Inhibitors