Increased expression and activity of group IIA and X secretory phospholipase A2 in peritumoral versus central colon carcinoma tissue.
Secretory phospholipase A2 (sPLA2) type IIA and X was analyzed in tumors from 22 patients with colon adenocarcinomas in order to determine the involvement and activity of sPLA2 in colon cancer. Evaluation of immunoreactive sPLA2 IIA by Western blotting showed a significantly higher level in the periphery of the tumors, compared to central tumor regions. Increased levels of sPLA2 IIA protein correlated with a two-fold increase in sPLA2 enzymatic activity in the peripheral regions compared to central regions. Nineteen out of 22 tumors showed high levels of sPLA2 IIA, whereas 7 out of the 22 tumors showed sPLA2 type X. These data demonstrate that both sPLA2 type IIA and X are present in human colon cancer and suggest a role for sPLA2 in colon cancer tumor immunology and tumorigenesis.
Tribler
Line
L
LiPlasome Pharma AIS, Technical University of Denmark, DK-2800 Lyngby, Denmark.
Jensen
Lotte T
LT
Jørgensen
Kent
K
Brünner
Nils
N
Gelb
Michael H
MH
Nielsen
Hans Jørgen
HJ
Jensen
Simon S
SS
D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D015153:Blotting, Western; D003106:Colon / Q000201:enzymology; D003110:Colonic Neoplasms / Q000097:blood / Q000201:enzymology* / Q000473:pathology / Q000601:surgery; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D054501:Group II Phospholipases A2 / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism*; D054509:Group X Phospholipases A2 / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism*; D006801:Humans; D008297:Male; D008321:Mammary Glands, Animal / Q000201:enzymology; D008875:Middle Aged; D051381:Rats; D017207:Rats, Sprague-Dawley
D054501:Group II Phospholipases A2; D054509:Group X Phospholipases A2