title: Late-bolting transgenic Chinese cabbage obtained by RNA interference technique.
abstract: LEAFY (LFY) gene plays an important role in determining plant flowering mainly by controlling the timing of phase transition. Constitutive under-expression of LFY in Arabidopsis resulted in the formation of a late-flowering and highly branching phenotype. In this paper, an RNAi approach was used in down-regulated LFY gene expression to delay Chinese cabbage (Brassica rapa L. ssp. pekinensis) bolting and flowering. The results show that transgenic plant has a later transition to the reproductive phase, and the transgenic plants have more branches, more leaves, but a lower height. Results of RQ-RT-PCR analysis show that LFY gene expression was greatly reduced in transgenic plants. These results suggest that inhibiting LFY gene expression by RNA interference can delay bolting in a cold-sensitive long-day (LD) condition. Late flowering of Chinese cabbage can be used as a good genetic resource for the breeding late-bolting Chinese cabbage.
authors.affiliation: Biology Department, Tonghua Normal University, Tonghua, Jilin 134001, China.
authors.forename: Guang-Qing
authors.initials: GQ
authors.lastname: Xia
authors.forename: Jun-Yi
authors.initials: JY
authors.lastname: Zhu
authors.forename: Qi-Wei
authors.initials: QW
authors.lastname: He
authors.forename: Shuang-Yi
authors.initials: SY
authors.lastname: Zhao
authors.forename: Cui-Hua
authors.initials: CH
authors.lastname: Wang
mesh_terms: D015139:Blotting, Southern; D001937:Brassica / Q000235:genetics* / Q000254:growth & development*; D017343:Genes, Plant / Q000235:genetics; D008957:Models, Genetic; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development*; D034622:RNA Interference / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction