Basolateral budding of Marburg virus: VP40 retargets viral glycoprotein GP to the basolateral surface.
Virus budding from the basolateral domain of infected polarized cells could be one of the mechanisms underlying the quick systemic infection induced by Marburg virus (MARV). We found that MARV buds from the basolateral pole of hepatocytes and bile epithelial cells in infected guinea pigs, which leads to the release of infectious virus into the vascular system. Basolateral budding might be orchestrated by the basolaterally located MARV matrix protein VP40, which induces a partial relocalization of MARV glycoprotein from the apical to the basolateral plasma membrane. This redistribution is a prerequisite for budding of infectious virions from the basolateral domain.
Kolesnikova
Larissa
L
Institute for Virology, Philipps-Universitat Marburg, Marburg, Germany.
Ryabchikova
Elena
E
Shestopalov
Alexander
A
Becker
Stephan
S
D000818:Animals; D002460:Cell Line / Q000821:virology; D004195:Disease Models, Animal; D004285:Dogs; D006023:Glycoproteins / Q000235:genetics / Q000502:physiology*; D006168:Guinea Pigs; D007668:Kidney; D008379:Marburg Virus Disease / Q000821:virology*; D029024:Marburgvirus / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D009698:Nucleoproteins / Q000235:genetics / Q000502:physiology; D010957:Plasmids; D014162:Transfection; D014763:Viral Matrix Proteins / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*
D006023:Glycoproteins; D009698:Nucleoproteins; C080981:VP40 protein, virus; D014763:Viral Matrix Proteins; D014764:Viral Proteins