[Effects of exogenous human chorionic gonadotropin on nude mice bearing endometrial carcinoma].
OBJECTIVE
To observe the effects of exogenous human chorionic gonadotropin (hCG) on nude mice bearing transplanted endometrial carcinoma.


METHODS
Human endometrial carcinoma xenograft was transplanted in nude mice, and the effects of hCG injection on the tumor growth was evaluated according to tumorigenesis and xenograft weights. The expression of Ki-67 in the tumor was determined by immunohistochemistry, and HE staining was performed for morphological observation and measurement of the necrosis area in the tumor. The effect of hCG on fibrosis in the tumor was evaluated with Masson staining.


RESULTS
Compared to normal saline-treated tumor-bearing mice, the mice with hCG treatment showed increased tumor weight. HE staining for tumors in HCG-treatment group visualized tumor cell arrangement in glandular structure with smaller necrosis area, and Masson staining identified thick and compact collagen fibers as compared with the thin and loosely arranged fibers in saline-treated group. No significant difference was found in the Ki-67 expression in the tumors between the two groups.


CONCLUSIONS
Exogenous hCG can promote the differentiation of the endometrial carcinoma cells in vivo.
Zheng
Jun-Sheng
JS
Department of Gynecology and Obstetrics, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China. js-zheng@tom.com
Huang
Min
M
Lin
Chen-Li
CL
Xie
You-Ke
YK
Wang
Ming-Hua
MH
Zhong
Xue-Yun
XY
D000818:Animals; D045744:Cell Line, Tumor; D006063:Chorionic Gonadotropin / Q000627:therapeutic use*; D004195:Disease Models, Animal; D016889:Endometrial Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011897:Random Allocation; D023041:Xenograft Model Antitumor Assays
D006063:Chorionic Gonadotropin; D019394:Ki-67 Antigen