title: Multimers and adiponectin gene 276G>T polymorphism in the Japanese population residing in rural areas.
abstract: BACKGROUND
  Although it has been shown that high-molecular weight adiponectin is an active form, few studies have attempted to clarify the relationship between high molecular weight adiponectin and markers linked with cardiovascular diseases in the general population.
  
  
  METHODS
  We screened 236 Japanese study participants recruited from the general population, residing in one large and four small islands. In addition to serum lipids and lipoproteins, serum total adiponectin and each multimer were measured. The genotype single-nucleotide polymorphism 276G>T was detected in real-time PCR with LightCycler hybridization probes, using fluorescent-labeled nucleotides.
  
  
  RESULTS
  Multiple linear regression analysis showed that high-molecular weight adiponectin, as well as total adiponectin, were significantly correlated with body weight, body mass index, high-density lipoprotein cholesterol and triglycerides. Total adiponectin and high-molecular weight adiponectin concentrations were not significantly different between GG and TX (GT and TT) genotypes of 276G>T polymorphism in the adiponectin gene. Interestingly, no differences were observed for participants from the large island between GG and TX genotypes with regard to both total adiponectin and high-molecular weight adiponectin, whereas significant differences were observed for those from the small islands.
  
  
  CONCLUSIONS
  Our results show that total adiponectin and high-molecular weight adiponectin are associated with similar factors in the general population. Furthermore, different effects of 276G>T for participants from small and large islands suggest that regional background due to geographic barriers may control the effects of 276G>T on adiponectin concentrations.
authors.affiliation: Department of Island and Community Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Noboru
authors.initials: N
authors.lastname: Takamura
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Aoyagi
authors.forename: Hironori
authors.initials: H
authors.lastname: Yamasaki
authors.forename: Norio
authors.initials: N
authors.lastname: Abiru
authors.forename: Mio
authors.initials: M
authors.lastname: Nakazato
authors.forename: Shimeru
authors.initials: S
authors.lastname: Kamihira
authors.forename: Takahiro
authors.initials: T
authors.lastname: Maeda
mesh_terms: D052242:Adiponectin / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005838:Genotype; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012424:Rural Population
chemical_list: D052242:Adiponectin