[Effect of platelet rich plasma on vascularization of tissue-engineered bone].
OBJECTIVE
To study the effects of platelet rich plasma (PRP) on vascularization of tissue-engineered bone.


METHODS
Bone marrow stromal cell (BMSC) were isolated from iliac bone of dogs. PRP was obtained from the same dog and demineralized bone matrix (DBM) was prepared from homologous bone. Twelve dogs were divided into three groups and the back of each dog was divided into four areas. The DBM- BMSC- PRP was implanted in the area A and B; the DBM-BMSC without PRP was implanted in the area C and D. The implants in the areas A and C were wrapped using myo-fascia with blood vessel of latissimus dorsi. The implants in the area B and D were wrapped using superficial fascia of the back without blood vessel. The implants were taken out 4, 8 and 12 weeks later for examination.


RESULTS
The degree of calcification and blood vessel formation of the implants was A > B > C > D.


CONCLUSIONS
Both PRP and vessels of latissimus dorsi muscle could promote calcification and vascularization in tissue-engineered bone, when used separately or in combination.
Li
Ning-Yi
NY
Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Medical College, Qingdao University, Qingdao Shandong 266003, China. ningyili342@163.com
Chen
Li-Qiang
LQ
Chen
Tao
T
Jin
Xiao-Ming
XM
Yuan
Rong-Tao
RT
D000818:Animals; D001854:Bone Marrow Cells; D018786:Bone Substitutes; D002478:Cells, Cultured; D004285:Dogs; D018919:Neovascularization, Physiologic; D053657:Platelet-Rich Plasma; D017154:Stromal Cells; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D018786:Bone Substitutes