Several novel methods for immobilization of enzymes, microbial cells and organelles.
Two novel methods--"photo-crosslinkable resin prepolymer method" and "urethane prepolymer method"--have been developed in our laboratory. These methods have the following advantages : 1) Prepolymers of desired properties, such as optional chain length, hydrophilicity or hydrophobicity, and ionic character etc., can be used for entrapment of biocatalysts : (2) preparation of gel-entrapped biocatalysts can be easily achieved under very mild conditions. Photo-crosslinked gels are conveniently obtained by several minutes illumination with near-UV light, of a mixture of liquid prepolymers having photo-sensitive functional groups, an appropriate sensitizer and the solution or suspension of biocatalyst. Formation of polyurethane gels is completed by only mixing water-miscible urethane prepolymers with the aqueous solution or suspension of biocatalyst. The biocatalysts entrapped by these methods are useful for a variety of purposes.
Fukui
S
S
Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606, Japan.
Sonomoto
K
K
Itoh
N
N
Tanaka
A
A
D001671:Biochemistry / Q000379:methods / Q000639:trends*; D002384:Catalysis; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004800:Enzymes, Immobilized / Q000737:chemistry*; D007477:Ions; D008027:Light; D008956:Models, Chemical; D015388:Organelles / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D014466:Ultraviolet Rays; D014520:Urethane / Q000737:chemistry; D014867:Water / Q000737:chemistry
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D007477:Ions; D011108:Polymers; D014867:Water; D014520:Urethane