title: Znt7 (Slc30a7)-deficient mice display reduced body zinc status and body fat accumulation.
abstract: In vitro studies have demonstrated that ZNT7 is involved in transporting the cytoplasmic zinc into the Golgi apparatus of the cell for zinc storage or to be incorporated into newly synthesized zinc-requiring enzymes/proteins. To evaluate the physiological role of ZNT7, we created a mouse model of Znt7 deficiency by a gene-trap approach. Znt7-deficient mice were zinc-deficient based on their low zinc content in serum, liver, bone, kidney, and small intestine. In embryonic fibroblasts isolated from Znt7-deficient mice, cellular zinc was approximately 50% that of wild-type controls. Znt7-deficient mice also displayed some classic manifestations of dietary zinc deficiency, such as reduced food intake and poor body weight gain. However, the mutant mice did not show any sign of hair abnormality and dermatitis that are commonly associated with dietary zinc deficiency. A radioactive feeding study suggested that Znt7-deficient mice had reduced zinc absorption in the gut resulting in decreased zinc accumulations in other organs in the body. The poor growth found in Znt7-deficient mice could not be corrected by feeding the mutant mice with a diet containing 6-fold higher zinc (180 mg/kg) than the suggested adequate intake amount (30 mg/kg). Furthermore, the reduced body weight gain of the mutant mice was largely due to the decrease in body fat accumulation. We conclude that ZNT7 has essential functions in dietary zinc absorption and in regulation of body adiposity.
authors.affiliation: United States Department of Agriculture/Agriculture Research Service/Western Human Nutrition Research Center, Rowe Program in Genetics, University of California, Davis, California 95616, USA. liping.huang@ars.usda.gov
authors.forename: Liping
authors.initials: L
authors.lastname: Huang
authors.forename: Yan Yiu
authors.initials: YY
authors.lastname: Yu
authors.forename: Catherine P
authors.initials: CP
authors.lastname: Kirschke
authors.forename: Erik R
authors.initials: ER
authors.lastname: Gertz
authors.forename: Kent K C
authors.initials: KK
authors.lastname: Lloyd
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism* / Q000473:pathology; D050154:Adiposity / Q000187:drug effects / Q000235:genetics; D000327:Adsorption / Q000187:drug effects; D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000235:genetics; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism* / Q000473:pathology; D003872:Dermatitis / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D019587:Dietary Supplements; D004435:Eating / Q000235:genetics; D006056:Golgi Apparatus / Q000378:metabolism* / Q000473:pathology; D006197:Hair / Q000002:abnormalities / Q000378:metabolism; D017136:Ion Transport / Q000187:drug effects / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D009928:Organ Specificity / Q000235:genetics; D015032:Zinc / Q000172:deficiency / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D027682:Cation Transport Proteins; C472293:ZnT7 protein, mouse; D015032:Zinc