title: Polyurethane from biosource as a new material for fabrication of microfluidic devices by rapid prototyping.
abstract: This paper presents the use of elastomeric polyurethane (PU), derived from castor oil (CO) biosource, as a new material for fabrication of microfluidic devices by rapid prototyping. Including the irreversible sealing step, PU microchips were fabricated in less than 1h by casting PU resin directly on the positive high-relief molds fabricated by standard photolithography and nickel electrodeposition. Physical characterization of microchannels was performed by scanning electron microscopy (SEM) and profilometry. Polymer surface was characterized using contact angle measurements and the results showed that the hydrophilicity of the PU surface increases after oxygen plasma treatment. The polymer surface demonstrated the capability of generating an electroosmotic flow (EOF) of 2.6 x 10(-4)cm(2)V(-1)s(-1) at pH 7 in the cathode direction, which was characterized by current monitoring method at different pH values. The compatibility of PU with a wide range of solvents and electrolytes was tested by determining its degree of swelling over a 24h period of contact. The performance of microfluidic systems fabricated using this new material was evaluated by fabricating miniaturized capillary electrophoresis systems. Epinephrine and l-DOPA, as model analytes, were separated in aqueous solutions and detected with end-channel amperometric detection.
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil.
authors.forename: Evandro
authors.initials: E
authors.lastname: Piccin
authors.forename: Wendell Karlos Tomazelli
authors.initials: WK
authors.lastname: Coltro
authors.forename: José Alberto
authors.initials: JA
authors.lastname: Fracassi da Silva
authors.forename: Salvador Claro
authors.initials: SC
authors.lastname: Neto
authors.forename: Luiz Henrique
authors.initials: LH
authors.lastname: Mazo
authors.forename: Emanuel
authors.initials: E
authors.lastname: Carrilho
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods; D046209:Electrophoresis, Microchip / Q000379:methods*; D004837:Epinephrine / Q000032:analysis / Q000737:chemistry; D007980:Levodopa / Q000032:analysis / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D044085:Microfluidics / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D011140:Polyurethanes / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D011140:Polyurethanes; D007980:Levodopa; D004837:Epinephrine