title: Electrochemically controllable conjugation of proteins on surfaces.
abstract: The rational design of surfaces for immobilization of proteins is essential to a variety of biological and medical applications ranging from molecular diagnostics to advanced platforms for fundamental studies of molecular and cell biology. We have developed an advanced electrochemically based approach for site-selective and reaction-controlled immobilization of proteins on surfaces. When a molecular monolayer of 4-nitrothiophenol on gold electrode surfaces is reduced electrochemically in a selective fashion at its nitro groups, to afford amino groups by potentiometric scans, the amine can be employed to orchestrate the immobilization of proteins to the surface. This protein immobilization strategy could allow one to fabricate intricate protein structures on surfaces for addressing fundamental and applied problems in biology and medicine.
authors.affiliation: California NanoSystems Institute and Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA 90095-1569, USA. p.m.mendes@bham.ac.uk
authors.forename: Paula M
authors.initials: PM
authors.lastname: Mendes
authors.forename: Karen L
authors.initials: KL
authors.lastname: Christman
authors.forename: Puru
authors.initials: P
authors.lastname: Parthasarathy
authors.forename: Eric
authors.initials: E
authors.lastname: Schopf
authors.forename: Jianyong
authors.initials: J
authors.lastname: Ouyang
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Jon A
authors.initials: JA
authors.lastname: Preece
authors.forename: Heather D
authors.initials: HD
authors.lastname: Maynard
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: J Fraser
authors.initials: JF
authors.lastname: Stoddart
mesh_terms: D004563:Electrochemistry; D011506:Proteins / Q000737:chemistry*; D013057:Spectrum Analysis; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D011506:Proteins