title: SNPbox: web-based high-throughput primer design with an eye for repetitive sequences.
abstract: In this omics era, researchers in biosciences need more than ever user-friendly, fast, and straightforward computational tools for high-throughput research. In this chapter, we present SNPbox and explain the general primer design strategy. We also show how the four modules, Exon, Single-Nucleotide Polymorphism (SNP), Saturation, and Exon + Saturation, exactly apply the primer design strategy, give clear guidance for users of the Web interface at http://www.SNPbox.org, and explain how pre-designed primers for Ensembl genes can be visualized and retrieved.
authors.affiliation: Department of Molecular Genetics, Flanders Interuniversity Institute for Biotechnology (VIB), University of Antwerp, Belgium.
authors.forename: Stefan
authors.initials: S
authors.lastname: Weckx
authors.forename: Peter
authors.initials: P
authors.lastname: De Rijk
authors.forename: Wim
authors.initials: W
authors.lastname: Glassee
authors.forename: Christine
authors.initials: C
authors.lastname: Van Broeckhoven
authors.forename: Jurgen
authors.initials: J
authors.lastname: Del-Favero
mesh_terms: D017931:DNA Primers / Q000737:chemistry; D005091:Exons; D020407:Internet; D020641:Polymorphism, Single Nucleotide; D012091:Repetitive Sequences, Nucleic Acid; D014584:User-Computer Interface
chemical_list: D017931:DNA Primers