Co-regulation by Notch and Fos is required for cell fate specification of intermediate precursors during C. elegans uterine development.
The Notch pathway is the key signal for many cell fate decisions in the nematode Caenorhabditis elegans including the uterine pi cell fate, crucial for a proper uterine-vulval connection and egg laying. Expression of the egl-13 SOX domain transcription factor is specifically upregulated upon induction of the pi lineage and not in response to other LIN-12/Notch-mediated decisions. We determined that dual regulation by LIN-12 and FOS-1 is required for egl-13 expression at specification and for complete rescue of egl-13 mutants. We found that fos-1 mutants exhibit uterine defects and fail to express pi markers. We show that FOS-1 is expressed at pi cell specification and can bind in vitro to egl-13 upstream regulatory sequence (URS) as a heterodimer with C. elegans Jun.
Oommen
Kavita S
KS
Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Newman
Anna P
AP
D000818:Animals; D001665:Binding Sites; D019521:Body Patterning / Q000235:genetics*; D017173:Caenorhabditis elegans / Q000196:embryology* / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism; D012734:Disorders of Sex Development / Q000196:embryology / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005260:Female; D005786:Gene Expression Regulation; D008565:Membrane Proteins / Q000502:physiology*; D008954:Models, Biological; D032390:Myoblasts, Smooth Muscle / Q000378:metabolism / Q000502:physiology*; D016759:Oncogene Protein p65(gag-jun) / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism / Q000502:physiology*; D051881:Receptor, Notch1 / Q000502:physiology*; D051880:Receptors, Notch; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014599:Uterus / Q000196:embryology* / Q000378:metabolism
D029742:Caenorhabditis elegans Proteins; D004268:DNA-Binding Proteins; C491546:EGL-13 protein, C elegans; C520083:FOS-1 protein, C elegans; C099355:LAG-1 protein, C elegans; C082460:Lin-12 protein, C elegans; D008565:Membrane Proteins; D016759:Oncogene Protein p65(gag-jun); D016760:Proto-Oncogene Proteins c-fos; D051881:Receptor, Notch1; D051880:Receptors, Notch; D014157:Transcription Factors