Basic aspects of absorption and fluorescence spectroscopy and resonance energy transfer methods.
Absorption and fluorescence spectroscopy are the heart of life science laboratory techniques. These two spectroscopic tools are sensitive, are easy to use, and can provide a wide range of information. The goal of this chapter is to familiarize the reader with some basic principles and applications of absorption and steady-state fluorescence spectroscopy. Selected applications are illustrated with examples.
Shanker
Natasha
N
Department of Chemistry, State University of New York at Binghamton, Binghamton, New York 13902, USA.
Bane
Susan L
SL
D000042:Absorption; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006835:Hydrazones / Q000737:chemistry; D012996:Solutions; D012997:Solvents; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014443:Tyrosine / Q000737:chemistry
D006835:Hydrazones; D012996:Solutions; D012997:Solvents; D014443:Tyrosine