title: Effect of heparin on production of basic fibroblast growth factor and transforming growth factor-beta1 by human normal skin and hyperplastic scar fibroblasts.
abstract: Heparin affects both dermal fibroblast proliferation and collagen and may mediate these effects by altering the levels of basic fibroblast growth factor (bFGF) and transforming growth factor-beta1 (TGF-beta1) production as a wound healing modulator. The purpose of this study is to probe the effect of heparin on bFGF and TGF-beta1 production by human normal skin and hyperplastic scar fibroblasts. This research investigates the effect of heparin on bFGF and TGF-beta1 production by human normal skin and hyperplastic scar fibroblasts with exposure to 0, 100, 300, or 600 microg/ml heparin for 24, 48, 72, or 96 hours in a serum-free in vitro model. Levels of bFGF and TGF-beta1 in the supernatants were determined by enzyme-linked immunosorbant assay. All doses of heparin significantly stimulated production of bFGF by normal skin (393% to 1019% increase) and hyperplastic scar fibroblasts (405% to 899% increase) at all time points (P < .05). Heparin (300 and 600 microg/ml) also stimulated TGF-beta1 production by normal skin (26% to 83%) and hyperplastic scar fibroblasts (63% to 85%) with statistical significance (P < .05) at various time points. These effects of heparin on normal skin and hyperplastic scar fibroblasts may have implications for hyperplastic scar formation and wound healing in vivo.
authors.affiliation: Department of Burns and Plastic Surgery, The Second Affiliated Hospital of Shandong University, Shandong, China.
authors.forename: Shu-Qiang
authors.initials: SQ
authors.lastname: Fan
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Qin
authors.forename: Jing-Long
authors.initials: JL
authors.lastname: Cai
authors.forename: Gui-Ying
authors.initials: GY
authors.lastname: Zhu
authors.forename: Xu
authors.initials: X
authors.lastname: Bin
authors.forename: Hong-Shan
authors.initials: HS
authors.lastname: Yan
mesh_terms: D000328:Adult; D002921:Cicatrix / Q000188:drug therapy* / Q000517:prevention & control; D003094:Collagen / Q000187:drug effects; D005346:Fibroblast Growth Factors / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects*; D006493:Heparin / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D006965:Hyperplasia / Q000517:prevention & control*; D066298:In Vitro Techniques; D012867:Skin / Q000187:drug effects*; D053773:Transforming Growth Factor beta1 / Q000187:drug effects*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D053773:Transforming Growth Factor beta1; D005346:Fibroblast Growth Factors; D006493:Heparin; D003094:Collagen