title: Expression of three distinct families of calcium-activated chloride channel genes in the mouse dorsal root ganglion.
abstract: Objective A calcium-activated chloride current (IClCa) has been observed in medium-sized sensory neurons of the dorsal root ganglion (DRG). Axotomy of the sciatic nerve induces a similar current in the majority of medium and large diameter neurons. Our aim is to identify the molecule(s) underlying this current. Methods Using conventional and quantitative RT-PCR, we examined the expression in DRG of members of three families of genes, which have been shown to have IClCa current inducing properties. Results We showed the detection of transcripts representing several members of these families, i.e. chloride channel calcium-activated (CLCA), Bestrophin and Tweety gene families in adult DRG, in the normal state and 3 d after sciatic nerve section, a model for peripheral nerve injury. Conclusion Our analysis revealed that that mBest1 and Tweety2 appear as the best candidates to play a role in the injury-induced IClCa in DRG neurons.
authors.affiliation: The French National Institute for Health and Medical Research, U.583, Montpellier, France. mjumaily@univ-montp2.fr
authors.forename: Mohammed
authors.initials: M
authors.lastname: Al-Jumaily
authors.forename: Alexei
authors.initials: A
authors.lastname: Kozlenkov
authors.forename: Ilana
authors.initials: I
authors.lastname: Mechaly
authors.forename: Agnes
authors.initials: A
authors.lastname: Fichard
authors.forename: Valerie
authors.initials: V
authors.lastname: Matha
authors.forename: Frederique
authors.initials: F
authors.lastname: Scamps
authors.forename: Jean
authors.initials: J
authors.lastname: Valmier
authors.forename: Patrick
authors.initials: P
authors.lastname: Carroll
mesh_terms: D000818:Animals; D019771:Axotomy; D018118:Chloride Channels / Q000096:biosynthesis* / Q000235:genetics*; D017931:DNA Primers; D005727:Ganglia, Spinal / Q000378:metabolism*; D015870:Gene Expression; D051379:Mice; D009475:Neurons, Afferent / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012584:Sciatic Nerve / Q000502:physiology
chemical_list: D018118:Chloride Channels; D017931:DNA Primers