Tryptophan supplementation and pH adjustment for optimizing the sporulation of Coniothyrium minitans.
To determine the effect of tryptophan and pH on sporulation of Coniothyrium minitans, the fungus was cultivated using a two-stage, agar plate method in which addition of tryptophan and pH were controlled at the sporulation stage. The spore yield was enhanced by 4 times with 0.1 g tryptophan/l addition after 72 h. The optimal pH values were 4 for mycelia growth and 5.8-6 for sporulation. Mycelia grown at pH 6 had a higher productivity of spore production than did those grown at pH 4.
Shi
Junling
J
College of Food Science and Engineering, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China. sjlshi2004@yahoo.com.cn
Li
Yin
Y
Zheng
Yi
Y
Zhu
Yi
Y
Zhang
Xiaoping
X
Du
Guocheng
G
Chen
Jian
J
D001203:Ascomycota / Q000187:drug effects / Q000254:growth & development*; D006863:Hydrogen-Ion Concentration; D013172:Spores, Fungal / Q000187:drug effects / Q000254:growth & development; D014364:Tryptophan / Q000494:pharmacology*
D014364:Tryptophan