Identification of putative Arabidopsis DEMETER target genes by GeneChip analysis.
The Arabidopsis DEMETER (DME) DNA glycosylase is required for the maternal allele expression of imprinted Polycomb group (MEDEA and FIS2) and transcription factor (FWA) genes in the endosperm. Expression of DME in the central cell, not in pollen or stamen, establishes gene imprinting by hypomethylating maternal alleles. However, little is known about other genes regulated by DME. To identify putative DME target genes, we generated CaMV:DME plants which ectopically express DME in pollen and stamens. Comparison of mRNA profiles revealed 94 genes induced by ectopic DME expression in both stamen and pollen. Gene ontology analysis identified three molecular functions enriched in the DME-inducible RNA list: DNA or RNA binding, kinase activity, and transcription factor activity. Semi-quantitative RT-PCR verified the candidate genes identified by GeneChip analysis. The putative target genes identified in this study will provide insights into the regulatory mechanism of DME DNA glycosylase and the functions of DNA demethylation.
Ohr
Hyonhwa
H
Department of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
Bui
Anhthu Q
AQ
Le
Brandon H
BH
Fischer
Robert L
RL
Choi
Yeonhee
Y
D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D018390:Gene Targeting / Q000379:methods*; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015534:Trans-Activators / Q000235:genetics*
D029681:Arabidopsis Proteins; D015534:Trans-Activators; C463261:DEMETER protein, Arabidopsis; D009699:N-Glycosyl Hydrolases