Hepatoprotective and Antioxidant Effects of Ethanol Extract from Phellinus merrillii on carbon tetrachloride-induced liver damage.
In the present study, we investigated the hepatoprotective and antioxidant capacities of ethanol extract of Phellinus merrillii (PM) on carbon tetrachloride-induced hepatotoxicity. In high-performance liquid chromatography (HPLC) analysis, the finger print chromatogram of PM was established. Both hispolon and PM showed a similar peak at the retention time of 6 min. This implied that PM did contain the active ingredient of hispolon. Treatment with PM (0.5, 1 and 2 g/kg) prior to the administration of carbon tetrachloride (1.5 ml/kg in olive oil, 20%) significantly prevented the increased serum alanine aminotransferase (s-GOT) and serum aspartate aminotransferase (s-GPT) in a dose-dependent manner. We also found that the incidences of ballooning degeneration, necrosis and portal triaditis were lowered in the group pretreated with PM. Carbon tetrachloride induces up-regulation of antioxidant enzymes, including superoxide dismutase (SOD) (86.6%), catalase (58.8%) and glutathione peroxidase (GPx)(64.7%) in the liver. Pretreatment with PM significantly reduced the all these antioxidant enzyme activities. Therefore, we verified that ethanol extract of PM has the hepatoprotective and antioxidant capacities on rats.
Chang
Heng-Yuan
HY
Institute of Chinese Pharmaceutical Sciences, College of Pharmacy, China Medical University, 91, Hsueh-Shih Road, Taichung 404, Taiwan.
Peng
Wen-Huang
WH
Sheu
Ming-Jyh
MJ
Huang
Guan-Jhong
GJ
Tseng
Mu-Chuan
MC
Lai
Ming-Tsung
MT
Ho
Yu-Ling
YL
Chang
Yuan Shiun
YS
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D002252:Carbon Tetrachloride Poisoning / Q000201:enzymology / Q000503:physiopathology / Q000517:prevention & control*; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000503:physiopathology / Q000517:prevention & control*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry*; D008111:Liver Function Tests; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D000431:Ethanol