Evaluation of immunochromatography and commercial enzyme-linked immunosorbent assay for rapid detection of norovirus antigen in stool samples.
The efficiency of immunochromatography and commercial enzyme-linked immunosorbent assay (ELISA) kit (Denka Seiken Co. Ltd., Tokyo, Japan) were evaluated for rapid detection of norovirus (NoV) from stool specimens. A total of 503 stool specimens collected from infants and young children who suffered from acute gastroenteritis were tested for NoV by the NoV-immunochromatography kit, Denka ELISA kit, and by a monoplex RT-PCR method. The NoV-immunochromatography revealed 78.9% sensitivity, 96.4% specificity, and 92.4% efficiency with the monoplex RT-PCR method. The Denka ELISA kit had a sensitivity of 90.4%, specificity of 96.4%, and an efficiency level of 95%. The findings indicate that the newly developed NoV-immunochromatography kit provides the specificity equal to that of the Denka ELISA kit, even through the sensitivity of detection was lower. However, the advantage of the NoV-immunochromatography kit is less time consuming and simpler. The data show that both the Denka ELISA and the NoV-immunochromatography kits may be used as an alternative method for screening of NoV in stool samples.
Khamrin
Pattara
P
Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Nguyen
Tuan Anh
TA
Phan
Tung Gia
TG
Satou
Kenji
K
Masuoka
Yuichi
Y
Okitsu
Shoko
S
Maneekarn
Niwat
N
Nishio
Osamu
O
Ushijima
Hiroshi
H
D000956:Antigens, Viral / Q000302:isolation & purification; D017250:Caliciviridae Infections / Q000175:diagnosis* / Q000821:virology; D002648:Child; D002845:Chromatography / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005243:Feces / Q000821:virology*; D005759:Gastroenteritis / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D029322:Norovirus / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D000956:Antigens, Viral