The hermes transposon of Musca domestica is an efficient tool for the mutagenesis of Schizosaccharomyces pombe.
Currently, no transposon-based method for the mutagenesis of Schizosaccharomyces pombe exists. We have developed such a system based on the introduction of the hermes transposon from the housefly into S. pombe. This system efficiently disrupts open reading frames and allows the insertion sites to be readily identified.
Evertts
Adam G
AG
Section of Eukaryotic Transposable Elements, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Plymire
Christopher
C
Craig
Nancy L
NL
Levin
Henry L
HL
D000818:Animals; D001665:Binding Sites; D004251:DNA Transposable Elements; D006793:Houseflies / Q000235:genetics*; D016254:Mutagenesis, Insertional / Q000379:methods*; D016366:Open Reading Frames; D012568:Schizosaccharomyces / Q000235:genetics*
D004251:DNA Transposable Elements