HLA-A, -B, -DR haplotype frequencies from DNA typing data of 26,266 Chinese bone marrow donors.
HLA phenotypes of 26,266 Chinese individuals who were recruited as potential hematopoietic stem cell donors by the Shanghai Red Cross Marrow Donor Registry, part of the China Marrow Donor Program, were determined for HLA-A, -B, and -DRB1 alleles at low to intermediate resolution using DNA-based typing methods. The large sample size of the study allowed accurate calculation of the Chinese HLA haplotype frequencies. The observed alleles correspond to 19 HLA-A, 44 -B, and 13 -DR split antigens. The serologic equivalents of HLA-A36, -A80, -B78, and -DR18 alleles were not observed. A total of 2,241 distinct HLA-A, -B, -DRB1 haplotypes were identified. Three-locus haplotype frequency was estimated using the maximum likelihood method. The lowest haplotype frequency that can be reliably estimated at a 95% confidence level was 0.000057. Using this cutoff value, 1,220 haplotypes (54%) were statistically reliable and their cumulative haplotype frequency was 0.9730. The cumulative haplotype frequency of the remaining 1,021 haplotypes (46%) was 0.0270. A regression equation of p = 0.192 log N - 0.576 was derived to estimate the probability (p) of finding an HLA-A, -B, -DR split antigens-matched donor in a pool of N Chinese donors.
Du
Ke-Ming
KM
Shanghai Blood Center, Shanghai, China. dkeming@hotmail.com
Ji
Yun
Y
Xie
Jun-Hua
JH
Fu
Min
M
Sun
Ying
Y
Jin
Ye
Y
Sun
Ji-Li
JL
Yang
Jian-Hao
JH
Zhang
Zheng
Z
Mao
Zhen
Z
Liu
Da-Zhuang
DZ
Qian
Kai-Cheng
KC
Zhao
Tong-Mao
TM
D044466:Asian People / Q000235:genetics; D001853:Bone Marrow / Q000276:immunology*; D002681:China; D016172:DNA Fingerprinting; D005787:Gene Frequency; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D012042:Registries
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006684:HLA-DR Antigens