title: Development of high-throughput screening system by single-cell reaction using microchamber array chip.
abstract: To demonstrate the practical use of a novel high-throughput screening system by a single cell reaction for the rapid screening of combinatorially mutated beta-glucosidase 1 from Aspergillus oryzae, a yeast cell chip microchamber array in combination with yeast cell surface engineering was successfully developed.
authors.affiliation: Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui 910-8507, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fukuda
authors.forename: Michiko
authors.initials: M
authors.lastname: Kato-Murai
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Suye
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ueda
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D001617:beta-Glucosidase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D001617:beta-Glucosidase