title: Characterization of protein-protein interactions: application to the understanding of peroxisome biogenesis.
abstract: With the approaching completion of the Pichia pastoris genome, a greater emphasis will have to be placed on the proteome and the protein-protein interactions between its constituents. This chapter discusses methods that have been used for the study of such interactions among both soluble and membrane-associated proteins in peroxisome biogenesis. The procedures are equally applicable to other cellular processes.
authors.affiliation: Section of Molecular Biology, University of California San Diego, La Jolla, CA, USA.
authors.forename: Sebastien
authors.initials: S
authors.lastname: Leon
authors.forename: Ivet
authors.initials: I
authors.lastname: Suriapranata
authors.forename: Mingda
authors.initials: M
authors.lastname: Yan
authors.forename: Naganand
authors.initials: N
authors.lastname: Rayapuram
authors.forename: Amar
authors.initials: A
authors.lastname: Patel
authors.forename: Suresh
authors.initials: S
authors.lastname: Subramani
mesh_terms: D003432:Cross-Linking Reagents / Q000494:pharmacology; D005656:Fungal Proteins / Q000378:metabolism*; D047468:Immunoprecipitation; D020675:Peroxisomes / Q000187:drug effects / Q000378:metabolism*; D010843:Pichia / Q000187:drug effects / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects
chemical_list: D003432:Cross-Linking Reagents; D005656:Fungal Proteins