[The effects of nm23-H1 gene cell transfection on abilities of invasion, adhesion and chemotherapeutic sensitivity of bladder cancer T-24 cell line].
OBJECTIVE
To investigate the transfection and expression of nm23-H1 gene in human bladder cancer cell line T-24, and to find out whether nm23-H1 could affect the metastases ability and the chemotherapeutic sensitivity of T-24 cell line.


METHODS
By electroporation way, the pCMV-Bam-neo-nm23-H1 plasmid was transferred into bladder cancer line T-24, and the nm23-H1 vector-producer cell line T-24/nm23-H1 was isolated with G418 selection. The expression of nm23-H1 gene q,as detected by immunohistochemistry. The T-24 invasion, adherence and migration abilities between transfected and non-transfected cell lines were detected by transwell-room and wash techniques. The difference or change to the sensitivity of chemotherapeutic drug before and after cell transfected was evaluated by MTT.


RESULTS
The stable expression of nm23-H1 was obtained by pCMV-Bam-Neo system with G418. The expression of nucleoside diphosphate kinase-A (NDPKA) was observed for significant difference between transfected and non-transfected T-24 cell lines; The invasive, adherence and migration abilities of transfected T-24 cells were decreased more than those of non-transfected T-24 (P < 0. 05); The chemo-sensitivity of transfected T-24 line cells to cis-diamminedichloroplatinum (CDDP) increased significantly.


CONCLUSIONS
nm23-H1 gene can inhibit the metastasis of T-24 cell line and increase the chemo-sensitivity of T-24 cell line to CDDP significantly.
Cui
Shu
S
Department of Urology, the Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China.
Tang
Tie-long
TL
Li
Xiang
X
Li
Hong
H
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D005822:Genetic Vectors; D006801:Humans; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics*; D009362:Neoplasm Metastasis; D010957:Plasmids; D014162:Transfection; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy / Q000235:genetics*
D054778:NM23 Nucleoside Diphosphate Kinases; C517938:NME1 protein, human; D002945:Cisplatin