Differential accumulation of mRNAs in drought-tolerant and susceptible common bean cultivars in response to water deficit.
The physiological response to drought was measured in two common bean varieties with contrastive susceptibility to drought stress. A subtractive cDNA library was constructed from the two cultivars, Phaseolus vulgaris'Pinto Villa' (tolerant) and 'Carioca' (susceptible). 18 cDNAs displayed protein-coding genes associated with drought, cold and oxidative stress, signal transduction, plant defense, chloroplast function and unknown function. A cDNA coding for an aquaporin (AQP) was selected for further analyses. The open reading frames (ORFs) of AQPs from 'Pinto Villa' and 'Carioca' were compared and despite their similarity, accumulated differentially in the plant organs, as demonstrated by Northern blot and in situ hybridization. A phylogenetic analysis of the deduced amino acid sequence with other AQPs suggested a tonoplast-located protein. Under drought conditions, the levels of AQP mRNA from the susceptible cultivar decreased to undetectable levels; by contrast, 'Pinto Villa' mRNA was present and restricted the phloem tissue. This would allow 'Pinto Villa' to maintain vascular tissue functions under drought stress.
Montalvo-Hernández
Lourdes
L
Biotecnología y Bioingeniería, CINVESTAV IPN, Avenue. IPN 2508, San Pedro Zacatenco, 07360 México, DF.
Piedra-Ibarra
Elías
E
Biotecnología y Bioingeniería, CINVESTAV IPN, Avenue. IPN 2508, San Pedro Zacatenco, 07360 México, DF.|Laboratorio de Fisiología Vegetal, UBIPRO FESI UNAM, Iztacala, Edomex.
Gómez-Silva
Lidia
L
Biotecnología y Bioingeniería, CINVESTAV IPN, Avenue. IPN 2508, San Pedro Zacatenco, 07360 México, DF.
Lira-Carmona
Rosalía
R
Centro Medico Nacional Siglo XXI, IMSS, Av. Cuauhtemoc 330, Mexico DF.
Acosta-Gallegos
Jorge A
JA
Programa Nacional de Frijol, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Celaya, Mexico, Centro Bajío, México.
Vazquez-Medrano
Josefina
J
Laboratorio de Fisiología Vegetal, UBIPRO FESI UNAM, Iztacala, Edomex.
Xoconostle-Cázares
Beatriz
B
Biotecnología y Bioingeniería, CINVESTAV IPN, Avenue. IPN 2508, San Pedro Zacatenco, 07360 México, DF.
Ruíz-Medrano
Roberto
R
Biotecnología y Bioingeniería, CINVESTAV IPN, Avenue. IPN 2508, San Pedro Zacatenco, 07360 México, DF.
D000595:Amino Acid Sequence; D004190:Disasters; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D027805:Phaseolus / Q000235:genetics* / Q000378:metabolism*; D010788:Photosynthesis; D010802:Phylogeny; D018515:Plant Leaves / Q000166:cytology / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism; D018547:Plant Stems / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism*; D014867:Water / Q000378:metabolism*
D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D014867:Water