In vitro analysis of the TAT protein transduction domain as a drug delivery vehicle in protozoan parasites.
The present study was undertaken to analyse the capability of HIV-1 derived TAT protein transduction domain (PTD) fused with Green Fluorescent Protein (TAT-GFP) as a delivery vehicle into a range of protozoan parasites. Successful transduction of native purified TAT-GFP was observed by fluorescent microscopy in Cryptosporidium parvum, Giardia duodenalis, and Neospora caninum. The ability to transduce peptides and other cargo into protozoan parasites, will greatly assist in the delivery of future peptide-based drugs and target validation peptides.
Chin Lee
Stephanie Hui
SH
Division of Health Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia.
Jefferies
Ryan
R
Watt
Paul
P
Hopkins
Richard
R
Sotzik
Frank
F
Reid
Simon
S
Armson
Anthony
A
Boxell
Annika
A
Ryan
Una
U
D000818:Animals; D002415:Cats; D016785:Cryptosporidium parvum / Q000378:metabolism*; D016503:Drug Delivery Systems / Q000379:methods*; D005872:Giardia / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000502:physiology; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008856:Microscopy, Fluorescence; D018688:Neospora / Q000378:metabolism*; D014122:Toxoplasma / Q000378:metabolism*; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000502:physiology*
D054322:tat Gene Products, Human Immunodeficiency Virus; D049452:Green Fluorescent Proteins