title: Increased expression of hLRH-1 in human gastric cancer and its implication in tumorigenesis.
abstract: Altered signaling pathways or deregulated transcription factors represent an important category of molecular events leading to aberrant gene regulation in gastric cancer, among which the role of WNT/beta-catenin pathway remains unclear. LRH-1 is a critical transcription factor in controlling cell proliferation via crosstalk with the beta-catenin signaling pathway. In order to gain a knowledge of the expression of hLRH-1v1 and hLRH-1 in gastric cancer, a Q-PCR analysis was carried out. Our results showed that in about 50 and 47.6% of 42 tested patients with gastric cancer, the mRNA expression of hLRH-1v1 and hLRH-1 was significantly upregulated, as compared with self-paired normal control, respectively. Besides, overexpression of hLRH-1 was shown to promote the proliferation of gastric adenocarcinoma cell SGC-7901 via induction of cyclin E1. Taken together, our present study demonstrated for the first time the increased expression of hLRH-1v1 and hLRH-1 in human gastric cancer, an alteration which may implicate in tumorigenesis.
authors.affiliation: Research Center for Molecular Medicine, Institute of Laboratory Medicine, Fuzhou Dongfang Hospital, Fuzhou, Fujian 350025, PR China. shuiliangwang@yahoo.com.cn
authors.forename: Shui-Liang
authors.initials: SL
authors.lastname: Wang
authors.forename: De-Zhu
authors.initials: DZ
authors.lastname: Zheng
authors.forename: Feng-Hua
authors.initials: FH
authors.lastname: Lan
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Deng
authors.forename: Jian
authors.initials: J
authors.lastname: Zeng
authors.forename: Cheng-Jin
authors.initials: CJ
authors.lastname: Li
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Zhong-Yong
authors.initials: ZY
authors.lastname: Zhu
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D019927:Cyclin E / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D015854:Up-Regulation; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D019927:Cyclin E; D004268:DNA-Binding Proteins; C082631:NR5A2 protein, human; D020033:Protein Isoforms; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D051176:beta Catenin; D019938:Cyclin D1