title: Impact of salinomycin on the intestinal microflora of broiler chickens.
abstract: BACKGROUND
  The ionophoric coccidiostat salinomycin is widely used in chicken feed. In the near future the use of ionophore coccidiostats may be banned as has been the case for other antimicrobial growth promoters. This study was conducted to examine the effect of salinomycin on Campylobacter jejuni infection and on the composition of the caecal microflora in broiler chickens.
  
  
  METHODS
  An experimental infection study was carried out in isolators and the intestinal microflora was analyzed using quantitative cultivation, denaturant gradient gel electrophoresis (DGGE), cloning and sequencing.
  
  
  RESULTS
  We found no effect of salinomycin on C. jejuni but salinomycin significantly affected the composition of the microflora. In addition, salinomycin significantly reduced the prevalence of Clostridium perfringens and we observed a significant increase (62%) in the mean body weight of salinomycin treated chickens compared to un-treated controls.
  
  
  CONCLUSIONS
  Termination of the use of ionophore coccidiostats will not affect food safety related to campylobacter, but will increase the risk of necrotic enteritis in the broilers.
authors.affiliation: National Veterinary Institute, Technical University of Denmark, Hangøvej 2, DK-8200 Aarhus N, Denmark. cjo@vet.dtu.dk
authors.forename: Charlotte H
authors.initials: CH
authors.lastname: Johansen
authors.forename: Lotte
authors.initials: L
authors.lastname: Bjerrum
authors.forename: Karl
authors.initials: K
authors.lastname: Pedersen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D002169:Campylobacter Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000187:drug effects*; D002645:Chickens / Q000382:microbiology*; D005260:Female; D041981:Gastrointestinal Tract / Q000187:drug effects* / Q000382:microbiology*; D008297:Male; D011201:Poultry Diseases / Q000188:drug therapy* / Q000382:microbiology; D011714:Pyrans / Q000494:pharmacology*; D000095662:Polyether Polyketides
chemical_list: D000900:Anti-Bacterial Agents; D011714:Pyrans; C010327:salinomycin; D000095662:Polyether Polyketides