Glycosylinositolphosphoceramides in Aspergillus fumigatus.
Fungal glycosylinositolphosphoceramides (GIPCs) are involved in cell growth and fungal-host interactions. In this study, six GIPCs from the mycelium of the human pathogen Aspergillus fumigatus were purified and characterized using Q-TOF mass spectrometry and 1H, 13C, and 31P NMR. All structures have the same inositolphosphoceramide moiety with the presence of a C(18:0)-phytosphingosine conjugated to a 2-hydroxylated saturated fatty acid (2-hydroxy-lignoceric acid). The carbohydrate moiety defines two types of GIPC. The first, a mannosylated zwitterionic glycosphingolipid contains a glucosamine residue linked in alpha1-2 to an inositol ring that has been described in only two other fungal pathogens. The second type of GIPC presents an alpha-Manp-(1-->3)-alpha-Manp-(1-->2)-IPC common core. A galactofuranose residue is found in four GIPC structures, mainly at the terminal position via a beta1-2 linkage. Interestingly, this galactofuranose residue could be substituted by a choline-phosphate group, as observed only in the GIPC of Acremonium sp., a plant pathogen.
Simenel
Catherine
C
Unité de Résonance Magnétique Nucléaire des Biomolécules, CNRS URA 2185 Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris cedex 15, France.
Coddeville
Bernadette
B
Delepierre
Muriel
M
Latgé
Jean-Paul
JP
Fontaine
Thierry
T
D001232:Aspergillus fumigatus / Q000737:chemistry* / Q000378:metabolism; D006028:Glycosphingolipids / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D006028:Glycosphingolipids; C025524:inositolphosphoceramides