An E. coli expression system optimized for DELLA proteins.
The DELLA proteins are involved in regulation of plant growth in response to phytohormonal signals such as GA, ethylene, and auxin. They have become one of most challenging and active area of research due to their fundamental roles in plant biology. Here, we describe the first successful expression of the N-terminal domains of DELLA proteins of Arabidopsis thaliana and Malus domestica in Escherichia coli system which will be used to produce monoclonal antibodies for profiling protein micro-arrays. Optimizations of the cloning, expression, and purification using specific tags have been discussed.
Sun
Xiaolin
X
The Horticulture and Food Research Institute of New Zealand Ltd., Private Bag 11 030, Palmerston North, New Zealand. xsun@hortresearch.co.nz
Frearson
Nicky
N
Kirk
Christopher
C
Jones
William T
WT
Harvey
Dawn
D
Rakonjac
Jasna
J
Foster
Toshi
T
Al-Samarrai
Taha
T
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005822:Genetic Vectors; D005875:Gibberellins / Q000378:metabolism*; D027845:Malus / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification
D005875:Gibberellins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins