Multiple automated minibioreactor system for multifunctional screening in biotechnology.
The current techniques applied in biotechnology allow to obtain many types of molecules that must be tested on cell cultures (high throughput screening HTS). Although such tests are usually carried out automatically on mini or microwell plates, the procedures in the preindustrial stage are performed almost manually on higher volume recipients known as bioreactors. The growth conditions in both stages are completely different. The screening system presented in this work is based on the multiwell test plates philosophy, a disposable multiple minibioreactor that allows reproduction of industrial bioreactor culture conditions: aeration, stirring, temperature, O2, pH and visible range optical absorbance measurements. It is possible to reproduce the growth conditions for both suspended and adherent animal cell types using 1 to 10 ml vol. bioreactors. In the case of bacteria or yeast, it is not possible to achieve a high biomass concentration, due to the reduced head volume air supply.
Fontova
A
A
Electronic Engineering Dept., Technical University of Catalonia, Barcelona, Spain.
Soley
A
A
Gálvez
J
J
Sarró
E
E
Lecina
M
M
Rosell
J
J
Riu
P
P
Cairó
J
J
Gòdia
F
F
Bragós
R
R
D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D015195:Drug Design; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D012371:Robotics / Q000295:instrumentation* / Q000379:methods