Expression patterns of microRNAs 155 and 451 during normal human erythropoiesis.
To clarify the roles of microRNAs (miRNAs) in erythropoiesis, the expression of miR-155, miR-221, miR-223, and miR-451 were analyzed during the differentiation of purified normal human erythroid progenitors in a liquid culture system. Cells increased almost 500-fold in a number, and differentiated to benzidine-positive mature erythroblasts. Analyses of miRNA expression using the quantitative real-time polymerase chain reaction showed that the expression level of miR-155 decreased about 200-fold, and that the expression of miR-451 increased about 270-fold during 12 days of cultures. A moderate down-regulation of miR-221 and miR-223 was observed. MiR-451 was expressed in red blood cells about 10(4)-fold more than in granulocytes, obtained from normal human peripheral blood. These observations suggest that miR-155 and miR-451 are key molecules for normal erythroid differentiation, and that quantitative assays of the two miRNAs may be a relevant method for analyzing pathological erythropoiesis.
Masaki
Shizuka
S
Department of Health Sciences, Faculty of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812 8582, Japan.
Ohtsuka
Rie
R
Abe
Yasunobu
Y
Muta
Koichiro
K
Umemura
Tsukuru
T
D002454:Cell Differentiation; D002478:Cells, Cultured; D004912:Erythrocytes / Q000166:cytology* / Q000502:physiology*; D004920:Erythropoiesis / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics*
C524942:MIRN155 microRNA, human; D035683:MicroRNAs