SPR and ITC determination of the kinetics and the thermodynamics of bivalent versus monovalent sugar ligand-lectin interactions.
A kinetic study of the interaction of bivalent and monovalent sugar ligands with a lectin was undertaken with the aid of surface plasmon resonance (SPR) method. The study involved a series of bivalent alpha-D-mannopyranoside containing sugar ligands, with systematic variation in the distance between the sugar ligands. The detailed kinetic studies showed that bivalent ligands underwent a faster association (k(on)) and a slower dissociation (k(off)) of the ligand-lectin complexes, in comparison to the monovalent ligand-lectin complexes. The kinetic constants were complemented further by assessing the thermodynamic parameters with the aid of isothermal titration calorimetry (ITC). The initiation of cross-linking of ligand-lectin interactions emerge from the early stages of the complexation. The dynamic light scattering (DLS) and the transmission electron microscopy (TEM) techniques allowed judging the sizes and morphologies of the complex in the solution and solid states, respectively.
Murthy
Bandaru Narasimha
BN
Department of Organic Chemistry, Indian Institute of Science, Bangalore, 560 012, India.
Sinha
Sharmistha
S
Surolia
Avadhesha
A
Indi
Shantinath S
SS
Jayaraman
Narayanaswamy
N
D002151:Calorimetry; D002236:Carbohydrate Conformation / Q000528:radiation effects; D002241:Carbohydrates / Q000737:chemistry*; D007700:Kinetics; D037102:Lectins / Q000737:chemistry* / Q000648:ultrastructure; D008024:Ligands; D008027:Light; D046529:Microscopy, Electron, Transmission; D012542:Scattering, Radiation; D020349:Surface Plasmon Resonance; D013816:Thermodynamics
D002241:Carbohydrates; D037102:Lectins; D008024:Ligands