Modeling amino acid metabolism in mammalian cells-toward the development of a model library.
Amino acids are necessary to mammalian cell cultures both for protein synthesis and as an energy source. In this study, we present an unstructured mathematical model describing (i) cell growth and death kinetics and (ii) metabolism of glucose and 19 amino acids for HEK-293 and CHO IFN-gamma cell cultures. The proposed mathematical framework is in good agreement with experimental data for both cell lines. It accommodates the inclusion of expressions for other cellular activities, such as the production of recombinant viral vectors or proteins, and can be used as the basis for the development of a model library for mammalian cell cultures.
Kontoravdi
Cleo
C
Centre for Process Systems Engineering, Department of Chemical Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
Wong
Danny
D
Lam
Carolyn
C
Lee
Yih Yean
YY
Yap
Miranda G S
MG
Pistikopoulos
Efstratios N
EN
Mantalaris
Athanasios
A
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D016208:Databases, Factual; D005947:Glucose / Q000378:metabolism; D008962:Models, Theoretical
D000596:Amino Acids; D005947:Glucose