[Study of neurocytes apoptosis in vitro culture rat induced by aluminum].
OBJECTIVE
To study the rat neurocytes apoptosis induced by aluminum.


METHODS
0-3 day rats neurons, neuroglia cells and co-cultured neurocytes were cultured, which were treated by different concentration of AlCl3 x 6H2O, the cells by light microscope, fluorescent staining and fluorescent analysis were observed.


RESULTS
(1) Under light microscope and fluorescent staining, Al could cause strong typical morphology apoptosis changes in neurons, which appeared more earlier and typical than those in neuroglia cells and co-cultured neurocytes. (2) The results of fluorescent analysis reviewed that the early stage, late stage and total apoptosis rate of neurons apoptosis could be associated with Al concentration. However, Al could not induce neuroglia cells and co-cultured neurocytes apoptosis at the low and mid doses of aluminum, the apoptosis rates in four groups demonstrated no significance, in contrast only significant difference between control group and high dose group.


CONCLUSIONS
It was suggested that Al could induce apoptosis of neurocytes in rat.
Niu
Qiao
Q
Department of Occupational Health, Shanxi Medical University, Taiyuan 030001, China.
Zhang
Qinli
Q
Niu
Piye
P
Shi
Yingtao
Y
D000535:Aluminum / Q000633:toxicity*; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000633:toxicity; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D002712:Chlorides / Q000633:toxicity; D018920:Coculture Techniques; D009457:Neuroglia / Q000166:cytology*; D009474:Neurons / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D017607:Aluminum Compounds; D002712:Chlorides; D000077410:Aluminum Chloride; D000535:Aluminum