[Cytotoxicity of chimera peptides incorporating sequences of cyclin kinases inhibitors].
The study is concerned with proapoptotic properties of chimera peptides which incorporate sequences of inhibitors of cyclin kinases p161NK4a and p21CIP/WAF1 as well as internalized sequences (Antp and tat). Sequences of the p16 type appeared to be more cytotoxic than the p21 one. Cytotoxic effect proved dependent on orientation with respect to the C or N terminal point of a polypeptide chain rather than on chimera sequence extent. Although p16 endogenous synthesis did not influence chimera peptide levels, apoptosis did not take place in certain cellular lines. Due to the rather unsophisticated nature of such synthesis, it might be used in designing individually-tailored chemotherapeutic drugs.
Kharchenko
V P
VP
Kulinich
V G
VG
Lunin
V G
VG
Filiasova
E I
EI
Shishkin
A M
AM
Sergeenko
O V
OV
Riazanova
E M
EM
Voronina
O L
OL
Bozhenko
V K
VK
D000595:Amino Acid Sequence; D051943:Antennapedia Homeodomain Protein / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002678:Chimera; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D015696:Gene Products, tat / Q000378:metabolism*; D019942:Genes, p16; D016158:Genes, p53; D006801:Humans; D019169:Jurkat Cells; D008969:Molecular Sequence Data; D010455:Peptides / Q000494:pharmacology
D051943:Antennapedia Homeodomain Protein; C493956:CDKN1A protein, human; D019941:Cyclin-Dependent Kinase Inhibitor p16; D050759:Cyclin-Dependent Kinase Inhibitor p21; D015696:Gene Products, tat; D010455:Peptides