[Using ultra performance liquid chromatography].
An analytical method based on ultra performance liquid chromatography has been developed for the simultaneous determination of 15 hormones (triamcinolone, hydrocortisone, prednisone, cortisone, 6a-methylprednisolone, betamethasone, dexamethasone, prednisolone acetate, hydrocortisone acetate, estriol, beta-estradiol, estrone, diethylstilbestrol, testosterone and progesterone) in cosmetics. Various cosmetic samples were extracted with methanol under ultrasonication, and then cleaned up by a Oasis HLB solid phase extraction cartridge. The 15 hormones were separated and detected within 6 min on a Waters ACQUITY UPLC BEH C18 column (1.7 microm, 2.1 mm x 50 mm) by gradient elution with acetonitrile and water system. In the range of 1-25 ng, the calibration curves of 15 hormones showed good linearity with the correlation coefficients larger than 0.9995. The mean recoveries at the three spiked levels (2, 10, 20 mg/kg) were 88.2%-102.4% with the relative standard deviations of 1.6%-7.4%.
Ma
Qiang
Q
Institute of Industrial Product Inspection, Chinese Academy of Inspection and Quarantine, Beijing 100025, China. maqiang1129@yahoo.com.cn
Wang
Chao
C
Wang
Xing
X
Bai
Hua
H
Chen
Wei
W
Wu
Ting
T
Zhou
Xin
X
Yu
Wenlian
W
D001623:Betamethasone / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D003358:Cosmetics / Q000032:analysis*; D003907:Dexamethasone / Q000032:analysis; D004054:Diethylstilbestrol / Q000032:analysis; D004964:Estriol / Q000032:analysis; D004970:Estrone / Q000032:analysis; D006728:Hormones / Q000032:analysis*; D006854:Hydrocortisone / Q000031:analogs & derivatives / Q000032:analysis; D008775:Methylprednisolone / Q000032:analysis; D011239:Prednisolone / Q000031:analogs & derivatives / Q000032:analysis; D011374:Progesterone / Q000032:analysis; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D013739:Testosterone / Q000032:analysis
D003358:Cosmetics; D006728:Hormones; D004970:Estrone; C021650:hydrocortisone acetate; D013739:Testosterone; D011374:Progesterone; D004054:Diethylstilbestrol; D003907:Dexamethasone; C009935:prednisolone acetate; D001623:Betamethasone; D011239:Prednisolone; D004964:Estriol; D006854:Hydrocortisone; D008775:Methylprednisolone