[Study on the effect of iodine excess on cell apoptosis in rat thyroid cells (FRTL)].
OBJECTIVE
To explore the mechanism of iodine over-treatment to FRTL cell on the cell apoptosis through observing the cell apoptosis related gene.


METHODS
The cells were treated with potassium iodide (0, 5, 10, 50 and 100mmol/L). After treatment 24 hours, the cells were collected and analyzed by flow cytometry. The mRNA levels of fas, fasL, Bcl-2 and Bax of 50mmol/L and 100mmol/L groups were determined with real-time PCR.


RESULTS
Compared with the normal control group, the rates of cell apoptosis of 10, 50 and 100mmol/L iodine groups increased significantly. Compared with the normal control group, the mRNA levels of fasL and Bax in 10 and 50mmol/L iodine groups increased significantly while the level of Bcl-2 decreased significantly. There was no significant difference fas mRNA levels between the normal control group and 10mmol/L iodine group, but the fas mRNA level in 50mmol/L iodine group increased significantly.


CONCLUSIONS
The excessive iodine treatment increased the apoptosis of FRTL cells which might be related to gene expressions of fas, fasL, Bcl-2 and Bax.
Xu
Jian
J
Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China. anniexu73@126.com
Liu
Xiaoli
X
Yang
Xuefeng
X
Li
Lanying
L
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D053222:Fas Ligand Protein / Q000235:genetics / Q000378:metabolism; D007452:Iodates / Q000494:pharmacology; D007455:Iodine / Q000494:pharmacology*; D017680:Potassium Compounds / Q000494:pharmacology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013961:Thyroid Gland / Q000166:cytology*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
C494828:Bax protein, rat; D053222:Fas Ligand Protein; C505662:Faslg protein, rat; D007452:Iodates; D017680:Potassium Compounds; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D007455:Iodine; C039693:potassium iodate