Changes of endocrine function of adipose tissue in anorexia nervosa: comparison of circulating levels versus subcutaneous mRNA expression.
OBJECTIVE
To study the influence of chronic malnutrition in patients with anorexia nervosa on endocrine function of adipose tissue on both circulating and subcutaneous fat mRNA expression level.


METHODS
A total of 12 patients with anorexia nervosa and 18 normal weight age-matched women underwent anthropometric examination, single blood drawing and subcutaneous adipose tissue biopsy.


METHODS
Serum concentrations of high-sensitive CRP (hsCRP), leptin, soluble leptin receptor, adiponectin, resistin, interleukin-6 and insulin were measured by Luminex, enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) kits. Subcutaneous adipose tissue mRNA expression of the same adipokines, adiponectin receptors 1 and 2 and immunocompetent cells marker CD68 was measured by real-time polymerase chain reaction (PCR).


RESULTS
Decreased body fat content of patients with anorexia nervosa was accompanied by reduced hsCRP, leptin and increased adiponectin and soluble leptin receptor. Resistin, interleukin-6 and insulin levels did not differ from those of the control group. Fat mRNA adiponectin, leptin, interleukin-6 and CD68 expression was reduced, resistin mRNA expression was increased and adiponectin receptor 1 and 2 expression were unchanged as compared to the control group.


CONCLUSIONS
Local perturbations in resistin, adiponectin and interleukin-6 mRNA expression in subcutaneous adipose tissue are not reflected by its circulating levels. These changes could be involved in some local metabolic disturbances in subcutaneous adipose tissue of anorexia nervosa patients.
Dolezalova
Radka
R
3rd Department of Medicine, 1st Faculty of Medicine and General University Hospital, Prague, Czech Republic.
Lacinova
Zdena
Z
Dolinkova
Marketa
M
Kleiblova
Petra
P
Haluzikova
Denisa
D
Housa
Daniel
D
Papezova
Hana
H
Haluzik
Martin
M
D000222:Adaptation, Physiological; D052242:Adiponectin / Q000097:blood* / Q000235:genetics*; D000328:Adult; D000856:Anorexia Nervosa / Q000276:immunology / Q000378:metabolism*; D015703:Antigens, CD / Q000235:genetics; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics; D001786:Blood Glucose / Q000032:analysis; D002097:C-Reactive Protein / Q000032:analysis; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D006801:Humans; D007328:Insulin / Q000097:blood; D015850:Interleukin-6 / Q000235:genetics; D044342:Malnutrition / Q000276:immunology / Q000378:metabolism; D019899:Paracrine Communication; D012333:RNA, Messenger / Q000032:analysis*; D054411:Receptors, Leptin / Q000097:blood; D052243:Resistin / Q000097:blood / Q000235:genetics; D018709:Statistics, Nonparametric; D050151:Subcutaneous Fat / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D000097382:CD68 Molecule
D052242:Adiponectin; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D001786:Blood Glucose; D002097:C-Reactive Protein; D007328:Insulin; D015850:Interleukin-6; D012333:RNA, Messenger; D054411:Receptors, Leptin; D052243:Resistin; C067980:CD68 antigen, human; D000097382:CD68 Molecule