Engineered cardiac tissues for in vitro assessment of contractile function and repair mechanisms.
For efficiently assessing the potential for grafted cells to repair infarcted myocardium, a simplified surrogate heart muscle system would offer numerous advantages. Using neonatal rat cardiac myocytes in a collagen matrix, we created thin cylindrical engineered cardiac tissues (ECTs) that exhibit essential aspects of physiologic cardiac muscle function. Furthermore, a novel cryo-injured ECT model of myocardial infarction offers the potential for the longitudinal study of mechanisms of cell-based cardiac repair in vitro.
Kim
Do Eun
DE
Department of Biomedical Engineering, Columbia University, 1210 Amsterdam Avenue, New York, NY 10027, USA. dk2194@columbia.edu
Lee
Eun Jung
EJ
Martens
Timothy P
TP
Kara
Rina
R
Chaudhry
Hina W
HW
Itescu
Silviu
S
Costa
Kevin D
KD
D000818:Animals; D000831:Animals, Newborn; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D006321:Heart / Q000502:physiology*; D009200:Myocardial Contraction / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*