[The formation of stable aggrregates of human platelets induced by lectin from Solanum tuberosum].
It has been demonstrated for the first time that GlcNAc-specific lectin from Solanum tuberosum induces the formation of haptenic sugar-resistant intercellular contacts (HSR-contacts) in platelet aggregation and does not induce stable neutrophil and lymphocyte aggregation. The formation of HSR-contacts in platelets was significantly impaired by the inhibitors of cAMP phosphodiesterase (papaverine) and arachidonic acid methabolism (indomethacin, aristolochic acid, and MK-886) as well as by the sulfhydryl reagent N-ethylmaleimide. The results obtained indicate that STA can be used to study the mechanisms of stable platelet aggregation, to screen drugs with potential antithrombotic activity, and to develop new cell engineering techniques.
Gorudko
I V
IV
Loĭko
E N
EN
Cherenkevich
S N
SN
Timoshenko
A V
AV
D001792:Blood Platelets; D002450:Cell Communication; D004353:Drug Evaluation, Preclinical; D006801:Humans; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism*; D010208:Papaverine / Q000494:pharmacology; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D037121:Plant Lectins / Q000737:chemistry / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D011198:Solanum tuberosum / Q000737:chemistry*; D013927:Thrombosis / Q000188:drug therapy / Q000378:metabolism
D016859:Lipoxygenase Inhibitors; D010726:Phosphodiesterase Inhibitors; D037121:Plant Lectins; D010208:Papaverine