title: Effect of UV irradiation on stabilized collagen: role of chromium(III).
abstract: Research on the effect of UV radiation on stabilized collagen is an area of potential interest owing to the fact that collagen is an important biomaterial finding immense use in various fields. In this present study, effect of UV irradiation on collagen stabilized using chromium(III) has been studied. The physical and optical properties affected by UV irradiation have been detailed. Viscosity measurements have shown that chromium(III) treated collagen has better stability against UV radiation than native collagen. Circular dichroic studies indicate that increase in concentration of chromium(III) does not affect the conformation of collagen however, the duration of irradiation has profound impact on the conformation of collagen. The fluorescence intensity of native collagen has been found to decrease more than that of chromium(III) treated collagen. The difference absorption spectra also shows that chromium(III) treatment brings about more stability to collagen against UV irradiation.
authors.affiliation: Chemical Lab, Central Leather Research Institute, Adyar, Chennai 20, India.
authors.forename: Nishtar Nishad
authors.initials: NN
authors.lastname: Fathima
authors.forename: Raju
authors.initials: R
authors.lastname: Suresh
authors.forename: Jonnalagadda Raghava
authors.initials: JR
authors.lastname: Rao
authors.forename: Balachandran Unni
authors.initials: BU
authors.lastname: Nair
authors.forename: Thirumalachari
authors.initials: T
authors.lastname: Ramasami
mesh_terms: D000818:Animals; D017608:Chromium Compounds / Q000494:pharmacology*; D002942:Circular Dichroism; D003094:Collagen / Q000187:drug effects / Q000528:radiation effects*; D008297:Male; D011487:Protein Conformation / Q000187:drug effects; D051381:Rats; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013431:Sulfates / Q000494:pharmacology*; D014466:Ultraviolet Rays; D014783:Viscosity
chemical_list: D017608:Chromium Compounds; D013431:Sulfates; C040874:chromium sulfate; D003094:Collagen