title: [Comparative study between 2 conjugates for the diagnosis of rabies in Cuba by direct immunofluorescence].
abstract: A comparison was made between the conjugate of national production made by "Carlos J. Finlay" Enterprise of Biological Products for diagnosing rabies by direct immunofluorescence and the viral antinucleocapsid conjugate manufactured by BIORAD that is commercialized at the international level. 150 samples of brain from different animal species were studied at the Rabies Reference Laboratory of "Pedro Kouri" Institute of Tropical Medicine from 2000 to 2002. On comparing both conjugates, there were obtained sensibility, specificity and concordance values of 100%, 94.3% and 98%, respectively. The discordant results were analyzed by the polymerase chain reaction and the biological test in mice.
authors.affiliation: Instituto de Medicina Tropical Pedro Kourí, Ciudad de La Habana, Cuba. maribas@ipk.sld.cu
authors.forename: María de los A Ribas
authors.initials: Mde L
authors.lastname: Antúnez
authors.forename: Gretel
authors.initials: G
authors.lastname: Acosta Renom
authors.forename: Yahisel
authors.initials: Y
authors.lastname: Tejero Suárez
authors.forename: Martínez
authors.initials: M
authors.lastname: Deneb García
authors.forename: Carina
authors.initials: C
authors.lastname: Rodríguez Valdez
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis*; D001921:Brain / Q000821:virology*; D002415:Cats; D002417:Cattle; D002685:Chiroptera; D003462:Cuba; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary*; D008988:Herpestidae; D051379:Mice; D019590:Nucleocapsid Proteins / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011818:Rabies / Q000175:diagnosis / Q000662:veterinary*; D011820:Rabies virus / Q000276:immunology / Q000302:isolation & purification*; D012377:Rodentia; D012680:Sensitivity and Specificity; D012756:Sheep; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins