title: Differential interleukin-6 messenger ribonucleic acid expression and its distribution pattern in eutopic and ectopic endometrium.
abstract: OBJECTIVE
  To evaluate the concentration and distribution pattern of interleukin-6-producing cells in ectopic and eutopic endometrium.
  
  
  METHODS
  Collection of both tissues at the proliferative cycle phase.
  
  
  METHODS
  A university endocrinology and laparoscopy program.
  
  
  METHODS
  Twelve samples from six women were obtained at operative laparoscopy in the proliferative phase of each woman's cycle.
  
  
  METHODS
  Paired sampling of ectopic (n = 6) and eutopic endometrium (n = 6).
  
  
  METHODS
  Concentration of IL-6 messenger RNA (mRNA) was measured by real-time RT-PCR, and its distribution was determined by in situ RT-PCR.
  
  
  RESULTS
  In vitro RT-PCR analysis showed distinct bands at 234 bp and 115 bp for IL-6 mRNA in eutopic and ectopic endometrium and in placenta as positive control. Real-time RT-PCR indicated significantly higher expression of IL-6 in tissue with endometriosis than in tissue without. In all 12 samples subjected to in situ RT-PCR, positive signals of IL-6 mRNA were observed in cytoplasm of epithelial and stroma cells of ectopic and eutopic endometrium tissue.
  
  
  CONCLUSIONS
  The significant IL-6 mRNA increase in endometriosis compared with in eutopic endometrium may play a role in the development of endometriosis and may provide new directions for endometriosis research.
authors.affiliation: Department of Obstetrics and Gynecology, University Hospitals Schleswig-Holstein, Campus Kiel, Kiel, Germany. endo-office@email.uni-kiel.des
authors.forename: Ali
authors.initials: A
authors.lastname: Salmassi
authors.forename: Yahya
authors.initials: Y
authors.lastname: Acil
authors.forename: Andreas Gerd
authors.initials: AG
authors.lastname: Schmutzler
authors.forename: Kerstin
authors.initials: K
authors.lastname: Koch
authors.forename: Walter
authors.initials: W
authors.lastname: Jonat
authors.forename: Liselotte
authors.initials: L
authors.lastname: Mettler
mesh_terms: D000328:Adult; D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution
chemical_list: D015850:Interleukin-6; D012333:RNA, Messenger