Origin of highly pathogenic H5N1 avian influenza virus in China and genetic characterization of donor and recipient viruses.
Genetic analysis of all eight genes of two Nanchang avian influenza viruses, A/Duck/Nanchang/1681/92 (H3N8-1681) and A/Duck/Nanchang/1904/92 (H7N1-1904), isolated from Jiangxi province, China, in 1992, showed that six internal genes of H3N8-1681 virus and five internal (except NS gene) genes of H7N1-1904 virus were closely similar to A/Goose/Guangdong/1/96 (H5N1) virus, the first highly pathogenic avian influenza (HPAI) virus of subtype H5N1 isolated in Asia. The neuraminidase (NA) gene of Gs/Gd/1/96 had the highest genetic similarity with A/Duck/Hokkaido/55/96 (H1N1-55) virus. The haemagglutinin (HA) gene of Gs/Gd/1/96 virus might have originated as a result of mutation of H5 HA gene from A/Swan/Hokkaido/51/96 (H5N3-51)-like viruses. The PA gene of H5N3-51 virus had the highest similarity with Gs/Gd/1/96. This study explains the origin of first Asian HPAI H5N1 virus in Guangdong by the reassortment of Nanchang (close to Guangdong) and Hokkaido (Japan) (H1N1-55 and H5N3-51) viruses. Genetic characteristics of donor and recipient viruses were also studied.
Mukhtar
Muhammad Mahmood
MM
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, P.R. China.
Rasool
Sahibzada T
ST
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, P.R. China.
Song
Degui
D
College of Life Sciences, Guangxi Normal University, Guilin 541004, P.R. China.
Zhu
Chengliang
C
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, P.R. China.
Hao
Qian
Q
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, P.R. China.
Zhu
Ying
Y
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, P.R. China.
Wu
Jianguo
J
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, P.R. China.
D000818:Animals; D002681:China; D004372:Ducks; D019143:Evolution, Molecular; D005777:Geese; D005814:Genes, Viral; D006389:Hemagglutinins, Viral / Q000235:genetics; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics; D053123:Influenza A Virus, H3N8 Subtype / Q000235:genetics; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005585:Influenza in Birds / Q000821:virology*; D009154:Mutation; D010802:Phylogeny; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D016865:Reassortant Viruses / Q000235:genetics* / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
D006389:Hemagglutinins, Viral; C061290:PA protein, influenza viruses; D014764:Viral Proteins; C458881:hemagglutinin fusogenic peptide, influenza virus; D012324:RNA-Dependent RNA Polymerase