Reshuffling of the Bacillus subtilis 168 genome by multifold inversion.
The genome of Bacillus subtilis 168 was modified to yield a genome vector for the cloning of DNA several Mb in size. Unlike contemporary plasmid-based vectors, this 4.2 Mb genome vector requires specific in vivo handling protocols because of its large size. Inversion mutagenesis, a method to modify local genome structure without gain or loss of genes, was applied intensively to the B. subtilis genome; this technique made possible both exchange and translocation of designated regions of the genome. This method not only reshuffles the genome of B. subtilis, but can provide insight into the biologic principles underlying genome plasticity.
Kuroki
Azusa
A
Graduate School of Media and Governance, Keio University, 5322 Endo, Fujisawa-shi, Kanagawa, 252-8520, Japan.
Toda
Tsutomu
T
Matsui
Kuniko
K
Uotsu-Tomita
Rie
R
Tomita
Masaru
M
Itaya
Mitsuhiro
M
D001412:Bacillus subtilis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D007446:Chromosome Inversion; D032621:DNA Shuffling; D024881:Drug Resistance, Bacterial; D016680:Genome, Bacterial; D009154:Mutation; D009355:Neomycin / Q000494:pharmacology
D009355:Neomycin