title: The effect of n-acetylcysteine and deferoxamine on exercise-induced oxidative damage in striatum and hippocampus of mice.
abstract: The aim of this study was to analyze the effects of intense exercise on brain redox status, associated with antioxidant supplementation of N-acetylcysteine (NAC), deferoxamine (DFX) or a combination of both. Seventy-two C57BL-6 adult male mice were randomly assigned to 8 groups: control, NAC, DFX, NAC plus DFX, exercise, exercise with NAC, exercise with DFX, and exercise with NAC plus DFX. They were given antioxidant supplementation, exercise training on a treadmill for 12 weeks, and sacrificed 48 h after the last exercise session. Training significantly increased (P < 0.05) soleus citrate synthase (CS) activity when compared to control. Blood lactate levels classified the exercise as intense. Exercise significantly increased (P < 0.05) oxidation of biomolecules and superoxide dismutase activity in striatum and hippocampus. Training significantly increased (P < 0.05) catalase activity in striatum. NAC and DFX supplementation significantly protected (P < 0.05) against oxidative damage. These results indicate intense exercise as oxidant and NAC and DFX as antioxidant to the hippocampus and the striatum.
authors.affiliation: Laboratory of Exercise Physiology, PPGCS, Universidade do Extremo Sul Catarinense, Criciuma, SC 88806-000, Brazil.
authors.forename: Aderbal S
authors.initials: AS
authors.lastname: Aguiar
authors.forename: Talita
authors.initials: T
authors.lastname: Tuon
authors.forename: Fernanda S
authors.initials: FS
authors.lastname: Soares
authors.forename: Luís Gustavo C
authors.initials: LG
authors.lastname: da Rocha
authors.forename: Paulo César
authors.initials: PC
authors.lastname: Silveira
authors.forename: Ricardo A
authors.initials: RA
authors.lastname: Pinho
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D002374:Catalase / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects* / Q000201:enzymology; D003676:Deferoxamine / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000201:enzymology; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects*; D010805:Physical Conditioning, Animal; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D002374:Catalase; D013482:Superoxide Dismutase; D003676:Deferoxamine; D000111:Acetylcysteine