[Sequence analysis of a novel HLA-A * 2459 allele].
This study was aimed to investigate the molecular genetics basis of a novel allele HLA-A * 2459 in Chinese population. DNA was extracted from whole blood by PEL-FREEZ DNA extraction kit. The amplification of HLA-A exons 2 - 4 of the proband was preformed by allele specific primer PCR and the amplified product was sequenced bidirectionally with primers. The sequencing results showed HLA-A alleles of the proband as A * 1101 and the novel allele. The sequences of the novel allele have been submitted to GenBank (DQ313255, DQ313256, DQ313257). After Blast HLA analysis, the novel allele showed only one nucleotide differences with HLA-A * 24020101 at nucleotide position 527 T to C in exon 3. This results in an amino acid changes from Val to Ala at codon 152. In conclusion, this allele is a novel one and has been officially named HLA-A * 2459 by the WHO Nomenclature Committee.
Zhang
Wei
W
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, China.
He
Jun-Jun
JJ
Lü
Qin-Feng
QF
Wang
Wei
W
Hang
Zhe-Dong
ZD
Zhu
Fa-Ming
FM
Yan
Li-Xing
LX
D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D001782:Blood Donors; D002681:China; D015234:HLA-A Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D015234:HLA-A Antigens