title: Characterisation of biofilm formation by a Streptococcus suis meningitis isolate.
abstract: Biofilm formation by a strain of Streptococcus suis serotype 2 isolated from a case of meningitis in pigs was characterised. Using a polystyrene microtitre plate assay, S. suis 95-8242 produced a dense biofilm when glucose, fructose or sucrose was used as the carbohydrate source, whereas no biofilm formed in the presence of lactose. Polysaccharide production by the biofilm-forming strain was demonstrated by the Congo red agar assay. Transmission electron microscopy revealed that bacterial cells were surrounded by a thick layer of polycationic ferritin-labelled material. S. suis 95-8242 was more resistant to both penicillin G and ampicillin in biofilms than in planktonic cultures on the basis of minimal inhibitory and minimal bactericidal concentrations.
authors.affiliation: Groupe de Recherche en Ecologie Buccale, Université Laval, Quebec City, Quebec, Canada G1K 7P4. Daniel.Grenier@greb.ulaval.ca
authors.forename: Daniel
authors.initials: D
authors.lastname: Grenier
authors.forename: Louis
authors.initials: L
authors.lastname: Grignon
authors.forename: Marcelo
authors.initials: M
authors.lastname: Gottschalk
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D018441:Biofilms / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial; D007700:Kinetics; D016920:Meningitis, Bacterial / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D013290:Streptococcal Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D017011:Streptococcus suis / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media