title: The role of dental pulp cells in resorption of deciduous teeth.
abstract: OBJECTIVE
  To address the question whether dental pulp cells of exfoliating human deciduous teeth have some roles for controlling or regulating the root resorption via secreting key molecules (OPG, RANKL, CSF-1, TGFbeta, MCP-1 and Cbfa-1) in osteoclastogenesis, we used a sensitive reverse transcriptase polymerase chain reaction (RT-PCR) method for detection of mRNA expressions for the cytokines listed.
  
  
  METHODS
  The dental pulps were retrieved from incisor and molar teeth in the late stage of shedding (n = 30) and from sound premolar teeth extracted for orthodontic reasons (control group; n = 30). The RT-PCR assays were used to identify targeted gene expression.
  
  
  RESULTS
  Of the cytokines examined, RANKL and CSF-1 expressions showed significantly higher occurrence in deciduous dental pulps than in permanent teeth pulpal tissues (P < .040).
  
  
  CONCLUSIONS
  The findings may suggest an interactive role for pulp tissue cells in the physiologic root resorption process. The cells of dental pulp may have some cytokine-producing cells which mediate monocyte-macrophage lineage to form osteo/odontoclasts, and the RANKL/RANK system might be involved in human deciduous teeth resorption.
authors.affiliation: Department of Pediatric Dentistry, Faculty of Dentistry, Selcuk University, Konya, Turkey. ysibel@msn.com
authors.forename: S
authors.initials: S
authors.lastname: Yildirim
authors.forename: M
authors.initials: M
authors.lastname: Yapar
authors.forename: U
authors.initials: U
authors.lastname: Sermet
authors.forename: K
authors.initials: K
authors.lastname: Sener
authors.forename: A
authors.initials: A
authors.lastname: Kubar
mesh_terms: D002648:Child; D017931:DNA Primers / Q000032:analysis; D003782:Dental Pulp / Q000166:cytology* / Q000000981:diagnostic imaging / Q000378:metabolism; D004812:Epidemiologic Methods; D005260:Female; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000032:analysis; D008297:Male; D053245:RANK Ligand / Q000032:analysis / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D011859:Radiography; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014080:Tooth Exfoliation / Q000000981:diagnostic imaging / Q000378:metabolism*; D014091:Tooth Resorption / Q000000981:diagnostic imaging / Q000378:metabolism*; D014094:Tooth, Deciduous / Q000000981:diagnostic imaging / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D053245:RANK Ligand; D012333:RNA, Messenger; C505688:TNFSF11 protein, human; D016173:Macrophage Colony-Stimulating Factor