Involvement of Fyn tyrosine kinase in actin stress fiber formation in fibroblasts.
Lysophosphatidic acid (LPA) and sphingosylphosphorylcholine (SPC) activated Fyn tyrosine kinase and induced stress fiber formation, which was blocked by pharmacological inhibition of Fyn, gene silencing of Fyn, or dominant negative Fyn. Overexpressed constitutively active Fyn localized at both ends of F-actin bundles and triggered stress fiber formation, only the latter of which was abolished by Rho-kinase (ROCK) inhibition. SPC, but not LPA, induced filopodia-like protrusion formation, which was not mediated by Fyn and ROCK. Thus, Fyn appears to act downstream of LPA and SPC to specifically stimulate stress fiber formation mediated by ROCK in fibroblasts.
Xu
Dan
D
Department of Molecular Physiology and Medical Bioregulation, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube 755-8505, Japan. jotan1995@yahoo.co.jp
Kishi
Hiroko
H
Kawamichi
Hozumi
H
Kajiya
Katsuko
K
Takada
Yuichi
Y
Kobayashi
Sei
S
D000199:Actins / Q000378:metabolism*; D000818:Animals; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008246:Lysophospholipids / Q000494:pharmacology; D051379:Mice; D041681:NIH 3T3 Cells; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000494:pharmacology; D051076:Proto-Oncogene Proteins c-fyn / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013110:Sphingosine / Q000031:analogs & derivatives / Q000494:pharmacology; D022502:Stress Fibers / Q000187:drug effects / Q000378:metabolism*; D014162:Transfection; D054460:rho-Associated Kinases / Q000378:metabolism
D000199:Actins; D008246:Lysophospholipids; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; C005356:sphingosine phosphorylcholine; D010767:Phosphorylcholine; C494957:FYN protein, human; D051076:Proto-Oncogene Proteins c-fyn; D054460:rho-Associated Kinases; D013110:Sphingosine; C032881:lysophosphatidic acid