title: [Effect of chitosan on the level of ROS in HepG2 cell induced by cadmium chloride].
abstract: OBJECTIVE
  To study the effect of chitosan on ROS levels in HepG2 cell induced by cadmium chloride.
  
  
  METHODS
  The experiments were divided into 6 groups: normal control group, cadmium chloride group (20micromol/L), chitosan group (0.50mg/ml) and three combined treatment group (cadmium chloride 20micromol/L and chitosan 0.02mg/ml, 0.10mg/ml and 0.50mg/ml). The HepG2 cells were treated by chitosan and cadmium chloride simultaneously for 4h. The fluorescence of 2', 7'-dichlorofluorescin (DCF) was measured by floweytometry as a mean of estimating the formation of reactive oxygen species (ROS).
  
  
  RESULTS
  In the comparison with normal control, the DCF intensity of chitosan group only exhibited somewhat decrease (P > 0.05) and significantly increased in cadmium chloride group (20micromol/L) (P < 0.01). The DCF intensity decreased in all three combined groups were more higher than those of cadmium chloride group, and when the concentration of chitosan improved from 0.01mg/ml to 0.50mg/ml, the DCF intensity decreased from 275.593 to 174.597 and the DCF intensity significant decrease was observed at concentration of 0.10mg/ml and 0.50mg/ml chitosan.
  
  
  CONCLUSIONS
  It was suggested that chitosan could antagonize the increase of ROS in HepG2 cell induced by cadmium chloride.
authors.affiliation: MOE Key Laboratory of Environment and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xie
authors.forename: Jiachun
authors.initials: J
authors.lastname: Wang
authors.forename: Guanyu
authors.initials: G
authors.lastname: Fan
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D019256:Cadmium Chloride / Q000037:antagonists & inhibitors / Q000633:toxicity*; D048271:Chitosan / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D056945:Hep G2 Cells; D006801:Humans; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D048271:Chitosan; D019256:Cadmium Chloride