Two-photon photostimulation and imaging of neural circuits.
We introduce an optical method to stimulate individual neurons in brain slices in any arbitrary spatiotemporal pattern, using two-photon uncaging of MNI-glutamate with beam multiplexing. This method has single-cell and three-dimensional precision. By sequentially stimulating up to a thousand potential presynaptic neurons, we generated detailed functional maps of inputs to a cell. We combined this approach with two-photon calcium imaging in an all-optical method to image and manipulate circuit activity.
Nikolenko
Volodymyr
V
Howard Hughes Medical Institute, Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, Box 2435, New York, New York 10027, USA. vn59@columbia.edu
Poskanzer
Kira E
KE
Yuste
Rafael
R
D000085:Acetates / Q000737:chemistry; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001931:Brain Mapping / Q000295:instrumentation / Q000379:methods*; D020013:Calcium Signaling / Q000187:drug effects; D003592:Cytophotometry / Q000295:instrumentation / Q000379:methods*; D004594:Electrophysiology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects; D005971:Glutamates / Q000378:metabolism; D005973:Glutamine / Q000494:pharmacology; D007211:Indoles / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D019579:Neocortex / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D009415:Nerve Net / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D015203:Reproducibility of Results; D013779:Tetrodotoxin / Q000494:pharmacology
C440256:4-methoxy-7-nitroindolinyl-glutamate; D000085:Acetates; D005971:Glutamates; D007211:Indoles; C405352:mag-indo-1 AM; D005973:Glutamine; C059587:Indo-1 pentaacetoxymethyl ester; D013779:Tetrodotoxin