title: Detection of functional ligand-binding events using synchrotron x-ray scattering.
abstract: Small-molecule ligands that change the structure of a protein are likely to affect its function, whereas those causing no structural change are less likely to be functional. Wide-angle x-ray scattering (WAXS) can be easily carried out on proteins and small molecules in solution in the absence of chemical tags or derivatives. The authors demonstrate that WAXS is a sensitive probe of ligand binding to proteins in solution and can distinguish between nonfunctional and productive binding. Furthermore, similar ligand-binding modes translate into similar scattering patterns. This approach has high potential as a novel, generic, low-throughput assay for functional ligand binding.
authors.affiliation: Biosciences Division, Argonne National Laboratory, Argonne, Illinois 60439,USA. drodi@anl.gov
authors.forename: Diane J
authors.initials: DJ
authors.lastname: Rodi
authors.forename: Suneeta
authors.initials: S
authors.lastname: Mandava
authors.forename: David B
authors.initials: DB
authors.lastname: Gore
authors.forename: Lee
authors.initials: L
authors.lastname: Makowski
authors.forename: Robert F
authors.initials: RF
authors.lastname: Fischetti
mesh_terms: D008024:Ligands; D012542:Scattering, Radiation; D017356:Synchrotrons
chemical_list: D008024:Ligands