Effect of surface properties of liposomes coated with a modified polyvinyl alcohol (PVA-R) on the interaction with macrophage cells.
The purpose of this study was to investigate the effect of a polymer coating using modified polyvinyl alcohol (PVA-R) on the interaction between liposomes and macrophage cells (J774 cells). The PVA-R-coated liposomes, which were labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocianin perchlorate (DiI) as a fluorescence reagent, were prepared with the conventional hydration method followed by extrusion and surface modification with PVA-R. The effects of polymer coating on the interaction with J774 cells were evaluated by using flow cytometry and confocal laser scanning microscopy (CLSM). When liposomes with or without PVA-R coating were incubated with J744 cells, the fluorescence emission intensity of DiI from J774 cells was significantly smaller than in the case of non-coated liposomes. This result indicates that decreased interaction of PVA-R-coated liposomes with macrophage cells could be well detected using flow cytometry and CLSM. These in vitro tests explained the differences in blood circulation of polymer-coated liposomes having different lipid formulations in rats.
Nakano
Koji
K
Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, Japan.
Tozuka
Yuichi
Y
Takeuchi
Hirofumi
H
D000818:Animals; D002232:Carbocyanines / Q000378:metabolism; D002478:Cells, Cultured; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000378:metabolism; D065347:Hypromellose Derivatives; D066298:In Vitro Techniques; D008081:Liposomes; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008747:Methylcellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D051379:Mice; D018613:Microscopy, Confocal; D011108:Polymers / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D013499:Surface Properties
D002232:Carbocyanines; D005456:Fluorescent Dyes; D008081:Liposomes; D011108:Polymers; D065347:Hypromellose Derivatives; C024286:3,3'-dioctadecylindocarbocyanine; D011142:Polyvinyl Alcohol; D008747:Methylcellulose