Caspase-2 cleaves DNA fragmentation factor (DFF45)/inhibitor of caspase-activated DNase (ICAD).
To investigate the signal transduction pathway of caspase-2, cell permeable Tat-reverse-caspase-2 was constructed, characterized and utilized for biochemical and cellular studies. It could induce the cell death as early as 2h, and caspase-2-specific VDVADase activity but not other caspase activities including DEVDase and IETDase. Interestingly, nuclear DNA fragmentation occurred and consistently DNA fragmentation factor (DFF45)/Inhibitor of caspase-activated DNase (ICAD) was cleaved inside the cell as well as in vitro, suggesting a role of caspase-2 in nuclear DNA fragmentation.
Dahal
Giri Raj
GR
Research Center for Proteineous Materials (RCPM), Department of Bio-materials Engineering, Chosun University, Gwangju 501-759, Republic of Korea.
Karki
Pratap
P
Thapa
Arjun
A
Shahnawaz
Mohammad
M
Shin
Song Yub
SY
Lee
Jung Sup
JS
Cho
Byungyun
B
Park
Il-Seon
IS
D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D053143:Caspase 2 / Q000378:metabolism*; D002470:Cell Survival / Q000502:physiology*; D004789:Enzyme Activation; D006367:HeLa Cells; D006801:Humans; D011506:Proteins / Q000378:metabolism*
D051017:Apoptosis Regulatory Proteins; D011506:Proteins; C109664:caspase-activated DNase inhibitor; D053143:Caspase 2