title: Auditory brainstem neural activation patterns are altered in EphA4- and ephrin-B2-deficient mice.
abstract: Auditory processing requires proper formation of tonotopically ordered projections. We have evaluated the role of an Eph receptor tyrosine kinase and an ephrin ligand in the development of these frequency maps. We demonstrated expression of EphA4 and ephrin-B2 in auditory nuclei and found expression gradients along the frequency axis in neonates. We tested the roles of EphA4 and ephrin-B2 in development of auditory projections by evaluating whether mutations result in altered patterns of expression of the immediate early gene c-fos after exposure to pure tone stimuli. We evaluated two nuclei, the dorsal cochlear nucleus (DCN) and the medial nucleus of the trapezoid body (MNTB), which project in two distinct auditory pathways. The mean number of c-fos-positive neurons in EphA4(-/-) DCN after 8-kHz pure tone stimulation was 42% lower than in wild-type DCN. Along the dorsoventral, tonotopic axis of DCN, the mean position of c-fos-positive neurons was similar for mutant and wild-type mice, but the spread of these neurons along the tonotopic axis was 35% greater for ephrin-B2(lacZ/+) mice than for wild-type mice. We also examined these parameters in MNTB after exposure to 40-kHz pure tones. Both EphA4(-/-) and ephrin-B2(lacZ/+) mice had significantly fewer c-fos-positive cells than wild-type littermates. The labeled band of cells was narrower and laterally shifted in EphA4(-/-) mice compared with wild-type mice. These differences in cell number and distribution suggest that EphA4 and ephrin-B2 signaling influence auditory activation patterns.
authors.affiliation: Department of Neurobiology and Behavior, University of California Irvine, Irvine, California 92697, USA.
authors.forename: Ilona J
authors.initials: IJ
authors.lastname: Miko
authors.forename: Paul A
authors.initials: PA
authors.lastname: Nakamura
authors.forename: Mark
authors.initials: M
authors.lastname: Henkemeyer
authors.forename: Karina S
authors.initials: KS
authors.lastname: Cramer
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D001306:Auditory Pathways / Q000002:abnormalities* / Q000166:cytology / Q000378:metabolism; D001307:Auditory Perception / Q000235:genetics; D019521:Body Patterning / Q000235:genetics*; D001933:Brain Stem / Q000002:abnormalities* / Q000166:cytology / Q000378:metabolism; D002452:Cell Count; D017626:Cochlear Nucleus / Q000002:abnormalities / Q000166:cytology / Q000378:metabolism; D036388:Ephrin-B2 / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout; D009474:Neurons / Q000378:metabolism; D009847:Olivary Nucleus / Q000002:abnormalities / Q000166:cytology / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D036122:Receptor, EphA4 / Q000235:genetics*
chemical_list: D036388:Ephrin-B2; D016760:Proto-Oncogene Proteins c-fos; D036122:Receptor, EphA4