Refining the sensory and motor ratunculus of the rat upper extremity using fMRI and direct nerve stimulation.
It is well understood that the different regions of the body have cortical representations in proportion to the degree of innervation. Our current understanding of the rat upper extremity has been enhanced using functional MRI (fMRI), but these studies are often limited to the rat forepaw. The purpose of this study is to describe a new technique that allows us to refine the sensory and motor representations in the cerebral cortex by surgically implanting electrodes on the major nerves of the rat upper extremity and providing direct electrical nerve stimulation while acquiring fMRI images. This technique was used to stimulate the ulnar, median, radial, and musculocutaneous nerves in the rat upper extremity using four different stimulation sequences that varied in frequency (5 Hz vs. 10 Hz) and current (0.5 mA vs. 1.0 mA). A distinct pattern of cortical activation was found for each nerve. The higher stimulation current resulted in a dramatic increase in the level of cortical activation. The higher stimulation frequency resulted in both increases and attenuation of cortical activation in different regions of the brain, depending on which nerve was stimulated.
Cho
Younghoon R
YR
Department of Plastic Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA.
Pawela
Christopher P
CP
Li
Rupeng
R
Kao
Dennis
D
Schulte
Marie L
ML
Runquist
Matthew L
ML
Yan
Ji-Geng
JG
Matloub
Hani S
HS
Jaradeh
Safwan S
SS
Hudetz
Anthony G
AG
Hyde
James S
JS
D000818:Animals; D004558:Electric Stimulation; D004566:Electrodes; D005552:Forelimb / Q000294:innervation*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008991:Monitoring, Physiologic; D051381:Rats