Exploring nociceptive response by BOLD fMRI in alpha-chloralose anesthetized rats.
The technique of blood oxygenation level dependent (BOLD) functional magnetic resonance imaging (fMRI) was used to provide a spatial-temporal mapping of nociceptive activation in brain. We contrived to obtain an illustration of the pain related regions by injecting formalin at the hindpaw using a 4.7 T MR system in alpha-chloralose anesthetized rats. In order to obtain the pain response, we avoided any invasive surgery on animals to purify the signal of nociception. The dynamic data were analyzed by mapping correlation coefficient and the time activity curves were calculated by atlas-based region of interest selection. The BOLD signals showed obvious difference in anterior cingulated cortex, somatosensory cortex, medial thalamus, and striatum after stimulation. The results not only show the global somatotopic organization of noxious stimulation on hindpaw in rats, but also provided invaluable information for neuroscience research.
Shih
Yen-Yu
YY
Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan. f93548021@ntu.edu.tw
Chen
You-Yin
YY
Chen
Jin-May
JM
Lung
Yun
Y
Chen
Jun-Ying
JY
Chang
Chen
C
Chen
Jyh-Cheng
JC
Jaw
Fu-Shan
FS
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000503:physiopathology*; D001931:Brain Mapping / Q000379:methods; D002698:Chloralose / Q000008:administration & dosage*; D005073:Evoked Potentials, Somatosensory; D005557:Formaldehyde; D006993:Hypnotics and Sedatives / Q000008:administration & dosage; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D009619:Nociceptors / Q000187:drug effects; D010101:Oxygen Consumption; D010146:Pain / Q000139:chemically induced* / Q000503:physiopathology*; D010147:Pain Measurement / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
D006993:Hypnotics and Sedatives; D005557:Formaldehyde; D002698:Chloralose