Use of polymerase chain reaction for the identification of Leptospira sp. in urine of carriers.
The objectives of the present work were to detect Leptospira seropositive animals. The ELISA results report only IgG antibodies, which could be attributable to chronic infections or else, that they are healthy carriers. All polymerase chain reaction positive animals should be considered potential sources of infection.
Ruiz
Victor Manuel Banda
VM
CENID Microbiología-INIFAP, México.
Vega
Laura Elena Orozco
LE
Velázquez
Rosa Maria Urrutia
RM
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000032:analysis; D002353:Carrier State / Q000382:microbiology*; D002417:Cattle / Q000382:microbiology*; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007919:Leptospira / Q000235:genetics* / Q000302:isolation & purification*; D007921:Leptospira interrogans / Q000276:immunology; D007922:Leptospirosis / Q000175:diagnosis / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D008800:Mexico; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D014556:Urine / Q000382:microbiology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D007074:Immunoglobulin G