Trypanosoma cruzi: antiprotozoal activity of parthenolide obtained from Tanacetum parthenium (L.) Schultz Bip. (Asteraceae, Compositae) against epimastigote and amastigote forms.
This study reports the activity of crude extracts, fractions and parthenolide (pure compound) obtained from Tanacetum parthenium against two forms of the parasite Trypanosoma cruzi. Feverfew is a traditional herbal medicine that has been used for the treatment of migraine, fever and arthritis. Activity against epimastigote forms was observed for crude extracts, fractions and parthenolide, and a progressive increase in the antitrypanosomal effect was observed in the course of the purification process. The pure compound showed IC50/96h and IC90/96h of 0.5 microg/ml and 1.25 microg/ml, respectively. The cytotoxic effect of parthenolide in LLMCK2 cells was 3.2 microg/ml (CC50/96h) and the selectivity index was 6.4. No hemolysis was detected for the pure compound. The internalization index of T. cruzi in LLMCK2 cells was reduced almost 51% at the concentration of 2 microg/ml of parthenolide, and 96.6% at 4 microg/ml. Scanning and transmission electron microscopy permitted observation of morphological modifications and ultrastructural alterations.
Izumi
Erika
E
Programa de Pós-graduação em Microbiologia, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445, Km 380, CEP 86051-990, Campus Universitário, Londrina, Paraná, Brazil.
Morello
Luis Gustavo
LG
Ueda-Nakamura
Tânia
T
Yamada-Ogatta
Sueli Fumie
SF
Filho
Benedito Prado Dias
BP
Cortez
Diógenes Aparício Garcia
DA
Ferreira
Izabel Cristina Piloto
IC
Morgado-Díaz
José Andrés
JA
Nakamura
Celso Vataru
CV
D000818:Animals; D000981:Antiprotozoal Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D020128:Inhibitory Concentration 50; D008253:Macaca mulatta; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010936:Plant Extracts / Q000494:pharmacology* / Q000633:toxicity; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D020903:Tanacetum parthenium / Q000737:chemistry*; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure
D000981:Antiprotozoal Agents; D010936:Plant Extracts; D012717:Sesquiterpenes; C002669:parthenolide