Beta7-integrin-independent enhancement of mucosal and systemic anti-HIV antibody responses following combined mucosal and systemic gene delivery.
Vaccination strategies that can block or limit heterosexual human immunodeficiency virus (HIV) transmissions to local and systemic tissues are the goal of much research effort. Herein, in a mouse model, we aimed to determine whether the enhancement of antibody responses through mucosal and systemic immunizations, previously observed with protein-based vaccines, applies to immunizations with DNA- or RNA-based vectors. Intranasal (i.n.) followed by intramuscular (i.m.) immunizations (i.n./i.m.) with polylactide-coglycolide (PLG)-DNA microparticles encoding HIV-gag (PLG-DNA-gag) significantly enhanced serum antibody responses, compared with i.m., i.n. or i.m. followed by i.n. (i.m./i.n.) immunizations. Moreover, while i.n./i.m., i.n. or i.m./i.n. immunizations with PLG-DNA-gag resulted in genital tract antibody responses, i.m. immunizations alone failed to do so. Importantly, beta7-deficient mice developed local and systemic antibody responses following i.n./i.m. immunization, or immunization via any other route, similar to those of wild-type mice. To compare the DNA with an RNA delivery system, immunizations were performed with VEE/SIN-gag replicon particles, composed of Venezuelan equine encephalitis virus (VEE) replicon RNA and Sindbis surface structure (SIN). i.n./i.m., compared with any other immunizations, i.n./i.m. immunization with VEE/SIN-gag resulted in enhanced genital tract but not serum antibody responses. These data show for the first time that mucosal followed by systemic immunizations with gene delivery systems enhance B-cell responses independent of the mucosal homing receptors alpha4beta7 and alphaEbeta7.
Goodsell
Amanda
A
Novartis Vaccines and Diagnostics, Inc., Emeryville, CA 94608, USA.
Zhou
Fengmin
F
Gupta
Soumi
S
Singh
Manmohan
M
Malyala
Padma
P
Kazzaz
Jina
J
Greer
Catherine
C
Legg
Harold
H
Tang
Tony
T
Zur Megede
January
J
Srivastava
Ranjana
R
Barnett
Susan W
SW
Donnelly
John J
JJ
Luciw
Paul A
PA
Polo
John
J
O'Hagan
Derek T
DT
Vajdy
Michael
M
D000281:Administration, Intranasal; D000818:Animals; D004666:Encephalitis Virus, Venezuelan Equine / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D018014:Gene Transfer Techniques; D015483:HIV Antibodies / Q000096:biosynthesis*; D015497:HIV-1 / Q000276:immunology*; D018928:Immunity, Mucosal; D007114:Immunization / Q000379:methods; D007273:Injections, Intramuscular; D039641:Integrin beta Chains / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011091:Polyesters; D012367:RNA, Viral / Q000276:immunology; D012093:Replicon / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology; D014621:Vagina / Q000276:immunology
D015483:HIV Antibodies; D039641:Integrin beta Chains; D011091:Polyesters; D012367:RNA, Viral; D019444:Vaccines, DNA; C091368:integrin beta7; C033616:poly(lactide)