Effect of maternal obesity on diabetes development in adult rat offspring.
This study aimed to evaluate whether maternal obesity leads to the onset of diabetes in adult Wistar rats offspring. MSG solution neonatally administration induced obesity in rats (F(1)MSG group, n=30); and saline solution was also administrated to control rats (F(1)CON group, n=13). In 3rd month of age, both control and MSG groups were mated for offspring (generation F(2)), named as F(2)CON, n=28 and F(2)MSG groups, n=15; and so both generations were studied until 7th month of life. Lee Index was measured for experimental obesity validation from 5th to 7th month. Glycemia was weekly determined during pregnancy and monthly from 3rd to 7th month. In the end of experimental period all rats were submitted to oral glucose tolerance test (OGTT), with estimation of total area under the curve (AUC); and insulin tolerance test (ITT). Rats were then anesthetized and killed. Data were statistically analyzed with significance level of p<0.05. Lee Index has confirmed obesity in all MSG rats. Glycemic levels comparisons between generations showed significant maternal interference in control and MSG groups. OGTT analysis showed higher glycemia in obese rats (F(1)MSG) and their offspring (F(2)MSG) as compared to their respective controls; and MSG groups increased AUC from OGTT. As regards ITT, F(2)MSG showed higher glycemia at 30 and 120 min, suggesting a delay of insulin action decreasing. Although glucose intolerance and insulin resistance clinical conditions represent as a factors for type 2 Diabetes mellitus development, this experimental model proposal was not efficient to induce type 2 Diabetes mellitus, but for obesity developing, glucose intolerance and insulin resistance in successive generations of rats.
de Campos
Kleber Eduardo
KE
Laboratory of Experimental Research of Gynecology and Obstetrics, Department of Gynecology and Obstetrics, School of Medicine of Botucatu--São Paulo State University (Unesp)--São Paulo State, CEP 18618-000, Brazil.
Sinzato
Yuri Karen
YK
Pimenta
Walkyria de Paula
Wde P
Rudge
Marilza Vieira Cunha
MV
Damasceno
Débora Cristina
DC
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000209:etiology* / Q000503:physiopathology; D005260:Female; D005951:Glucose Tolerance Test; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D009765:Obesity / Q000139:chemically induced / Q000150:complications* / Q000503:physiopathology; D011247:Pregnancy; D011248:Pregnancy Complications; D051381:Rats; D017208:Rats, Wistar; D012970:Sodium Glutamate / Q000633:toxicity; D013997:Time Factors
D001786:Blood Glucose; D012970:Sodium Glutamate