title: [Research on thymopentin-loaded N-trimethyl chitosan nanoparticles administered through mouth].
abstract: OBJECTIVE
  To prepare a peroral thymopentin-loaded N-trimethyl chitosan chloride-nanoparticle (Tp5-TMC-NP) ,and observe the pharmacodynamic action when the Tp5-TMC-NP is taken by way of the mouth.
  
  
  METHODS
  N-trimethyl chitosan chloride was first synthesized, and then Tp5-TMC-NP was prepared with the formulation technology optimized by the Central Composite Design. The influence of Tp5-TMC-NP on the ratio of CD4+/CD8+ of T-lymphocytes were determined by flow cytometer.
  
  
  RESULTS
  The regular global Tp5-TMC-NP prepared with the optimized formulation craft had the mean diameter of 110.6 nm and got the entrapment efficiency of 78.8%. The ratio of lymphocyte CD4+/CD8+ of Wistar rat administered with Tp5-TMC-NP perfusing stomach had 2.59 times higher than that with Tp5.
  
  
  CONCLUSIONS
  Taken orally the Tp5-TMC-NP has much higher efficiency than Tp5.
authors.affiliation: Key Laboratory of Drug Targeting, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
authors.forename: Shi-wei
authors.initials: SW
authors.lastname: Tang
authors.forename: Xiao-jia
authors.initials: XJ
authors.lastname: Yuan
authors.forename: Zhi-rong
authors.initials: ZR
authors.lastname: Zhang
authors.forename: Qing-guo
authors.initials: QG
authors.lastname: Song
authors.forename: Qin
authors.initials: Q
authors.lastname: He
mesh_terms: D000284:Administration, Oral; D000818:Animals; D016516:CD4-CD8 Ratio; D048271:Chitosan / Q000008:administration & dosage*; D004337:Drug Carriers / Q000008:administration & dosage*; D053758:Nanoparticles / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D016305:Thymopentin / Q000008:administration & dosage* / Q000493:pharmacokinetics
chemical_list: D004337:Drug Carriers; C118071:N-trimethyl chitosan chloride; D048271:Chitosan; D016305:Thymopentin