title: Equilibrium binding of proteins to F-actin.
abstract: This chapter reviews some of the many available methods for measuring the binding of myosin and other proteins to actin. Binding to actin has special considerations because actin is a long lattice and the binding site of many of its binding partners consists of multiple actin protomers. The analysis of binding to a lattice cannot be done by standard methods such as a Scatchard plot. Rational methods of analysis are described.
authors.affiliation: Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University, Greenville, NC, USA.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Chalovich
mesh_terms: D000199:Actins / Q000737:chemistry* / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D001671:Biochemistry / Q000379:methods*; D006801:Humans; D007477:Ions; D007700:Kinetics; D008024:Ligands; D015879:Myosin Subfragments / Q000737:chemistry; D011485:Protein Binding; D013696:Temperature
chemical_list: D000199:Actins; D007477:Ions; D008024:Ligands; D015879:Myosin Subfragments; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases