title: Anti-thyroperoxidase antibodies from patients with Hashimoto's encephalopathy bind to cerebellar astrocytes.
abstract: A cohort of 10 Hashimoto's encephalopathy (HE) patients, 33 patients with unrelated neurological symptoms, 12 Hashimoto's thyroiditis patients and 4 healthy adult donors was studied to explore the neurological targets of anti-thyroperoxidase (TPO) autoantibodies (aAb) in HE. High levels of anti-TPO aAb were only detected in HE group's cerebrospinal fluids. In immunofluorescence assays on monkey brain cerebellum sections, both HE patients' sera and anti-TPO monoclonal antibodies (mAb) were able to bind cerebellar cells expressing glial fibrillary acid protein. Normal human astrocytes from primary cultures also reacted with anti-TPO mAb. Specific astrocyte binding of anti-TPO aAb suggests a role of these aAb in the HE pathogenesis.
authors.affiliation: Laboratoire d'Immunologie et d'Immunopathologie, UPRES-EA 2128, CHU Clémenceau, 14033 Caen cedex, France. blanchin-s@chu-caen.fr
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Blanchin
authors.forename: Christine
authors.initials: C
authors.lastname: Coffin
authors.forename: Fausto
authors.initials: F
authors.lastname: Viader
authors.forename: Jean
authors.initials: J
authors.lastname: Ruf
authors.forename: Pierre
authors.initials: P
authors.lastname: Carayon
authors.forename: Francette
authors.initials: F
authors.lastname: Potier
authors.forename: Estelle
authors.initials: E
authors.lastname: Portier
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Comby
authors.forename: Stéphane
authors.initials: S
authors.lastname: Allouche
authors.forename: Yann
authors.initials: Y
authors.lastname: Ollivier
authors.forename: Yves
authors.initials: Y
authors.lastname: Reznik
authors.forename: Jean Jacques
authors.initials: JJ
authors.lastname: Ballet
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D001253:Astrocytes / Q000378:metabolism*; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D002478:Cells, Cultured; D002531:Cerebellum / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005333:Fetus; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D050031:Hashimoto Disease / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007453:Iodide Peroxidase / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay; D013954:Thyroglobulin / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology*; D013961:Thyroid Gland / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
chemical_list: D001323:Autoantibodies; D005904:Glial Fibrillary Acidic Protein; D013954:Thyroglobulin; D007453:Iodide Peroxidase