[Construction and immunogenicity study of DNA vaccine expressing human H5N1 influenza virus hemagglutinin].
Based on the first isolated human H5N1 influenza virus strain A/Anhui/1/2005 in China, HA and HA1 genes were amplified and cloned into the eukaryotic expression vector pStar. The recombinant plasmids pStar-HA and pStar-HA1 were transfected into COS7 cells. Western blot and IFA showed that the two recombinant DNA plasmids were successfully expressed in eukaryotic cells. BALB/c mice were immunized with the plasmids DNA by intramuscular injection. Anti-HA specific antibody in peripheral blood of immunized mice was tested by ELISA. The results showed that the recombinant plasmids successfully induced the anti-HA humoral immune response, and there was no significant difference between HA and HA1 as immunogen. This work provides basis for future development of novel avian flu vaccine.
Huan
Li-Fang
LF
National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China. lifanghuan99@hotmail.com
Yao
Li-Hong
LH
Chen
Ai-Jun
AJ
Cheng
Cong-Sheng
CS
Jia
Run-Qing
RQ
Tian
Qing
Q
Bo
Hong
H
Guo
Jian-Qiang
JQ
Wang
Min
M
Shu
Yue-Long
YL
Zhang
Zhi-Qing
ZQ
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D019556:COS Cells; D002522:Chlorocebus aethiops; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000276:immunology*
D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007074:Immunoglobulin G; D007252:Influenza Vaccines; D019444:Vaccines, DNA