[Are leukemic patient bone marrow mesenchymal stem cells malignant?].
The study was aimed to explore whether there are leukemic characteristics in the bone marrow mesenchymal stem cells (BMMSC) from leukemic patients as compared with normal controls. The mesenchymal stem cells from bone marrow of normal volunteers and patients with APL and CML were isolated, then cultured and proliferated in vitro. The morphology, growth curve and cell surface markers of two different sources mesenchymal stem cells were investigated for detecting whether the bone marrow mesenchymal stem cells derived from leukemia patients have the specific abnormal fusion gene of leukemia cells through fluorescent in situ hybridization. The results indicated that there was no significant difference between the mesenchymal stem cells derived from different subjects, the bone marrow mesenchymal stem cells derived from leukemia patients did not have the clonal malignant fusion gene as seen in the leukemia cells. Taken altogether, mesenchymal stem cells derived from leukemia patients had no biological differences as compared with those from normal volunteers, and no malignant clonal abnormality was found. It is concluded that mesenchymal stem cells derived from leukemia patients as an alternative vehicle may be used for assistant of autologous hematopoietic stem cell transplantation or cell therapy and gene therapy.
Xie
Zheng-Jun
ZJ
Department of Hematology, Kunming General Hospital, Chengdu Military Area, Kunming 650032, China.
Hu
Deng-Ming
DM
Wang-San-Bin
Yin
Bo
B
Zheng
Wei-Yang
WY
Xu
Bing
B
Xu
Xiao-Lan
XL
Lin
Rong
R
Feng
Ru
R
Zhou
Shu-Yun
SY
D001854:Bone Marrow Cells / Q000166:cytology; D002478:Cells, Cultured; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000473:pathology; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics / Q000473:pathology*; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D015514:Oncogene Proteins, Fusion / Q000235:genetics*
D015514:Oncogene Proteins, Fusion; C406060:abl-bcr fusion protein, human; C093476:promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein; D016044:Fusion Proteins, bcr-abl