[Comparative study between 2 conjugates for the diagnosis of rabies in Cuba by direct immunofluorescence].
A comparison was made between the conjugate of national production made by "Carlos J. Finlay" Enterprise of Biological Products for diagnosing rabies by direct immunofluorescence and the viral antinucleocapsid conjugate manufactured by BIORAD that is commercialized at the international level. 150 samples of brain from different animal species were studied at the Rabies Reference Laboratory of "Pedro Kouri" Institute of Tropical Medicine from 2000 to 2002. On comparing both conjugates, there were obtained sensibility, specificity and concordance values of 100%, 94.3% and 98%, respectively. The discordant results were analyzed by the polymerase chain reaction and the biological test in mice.
Antúnez
María de los A Ribas
Mde L
Instituto de Medicina Tropical Pedro Kourí, Ciudad de La Habana, Cuba. maribas@ipk.sld.cu
Acosta Renom
Gretel
G
Tejero Suárez
Yahisel
Y
Deneb García
Martínez
M
Rodríguez Valdez
Carina
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis*; D001921:Brain / Q000821:virology*; D002415:Cats; D002417:Cattle; D002685:Chiroptera; D003462:Cuba; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary*; D008988:Herpestidae; D051379:Mice; D019590:Nucleocapsid Proteins / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011818:Rabies / Q000175:diagnosis / Q000662:veterinary*; D011820:Rabies virus / Q000276:immunology / Q000302:isolation & purification*; D012377:Rodentia; D012680:Sensitivity and Specificity; D012756:Sheep; D013552:Swine
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins