Production of D-phenylglycine-related amino acids by immobilized microbial cells.
Bacterial dihydropyrimidinase was shown to catalyze the hydrolytic cleavage of various 5-substituted hydantoins to the corresponding N-carbamyl-D-amino acids under alkaline conditions. Therefore, an enzymatic method for preparing the D-forms of phenylglycine-related amino acids was developed using immobilized bacterial cells with high enzyme activity. Alkalophilic bacteria were a good enzyme source for this process. The process is simple and economical for use in the production of various amino acids with the D-configuration.
Yamada
H
H
Department of Agricultural Chemistry, Kyoto University, Kyoto, Japan.
Shimizu
S
S
Shimada
H
H
Tani
Y
Y
Takahashi
S
S
Ohashi
T
T
D000180:Acrylic Resins / Q000737:chemistry; D000581:Amidohydrolases / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry; D001407:Bacillus / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology; D001671:Biochemistry / Q000379:methods*; D002384:Catalysis; D018914:Cells, Immobilized; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry; D006827:Hydantoins; D006868:Hydrolysis; D008956:Models, Chemical; D013379:Substrate Specificity; D014508:Urea / Q000031:analogs & derivatives / Q000737:chemistry; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000581:Amidohydrolases; D000596:Amino Acids; D005998:Glycine; D006827:Hydantoins; D014508:Urea; D000099166:Polyacrylamides; C011271:hydantoic acid; C008852:2-phenylglycine; C020047:dihydropyrimidinase