[Expression of beta-Catenin Gene in CML and its relationship with bcr/abl].
This study was aimed to quantitatively detect the expression level of beta-catenin and bcr/abl in different phases of chronic myeloid leukemia (CML) and to analyze their potential relationship and significance in the progression of CML. First, the total RNA isolated from BMMNC of patients with CML and donors was reversely transcribed into cDNA. The real-time quantitative PCR method was used to analyze the expression level of beta-catenin and bcr/abl. The expression level of beta-catenin and bcr/abl in different phases of CML was compared and the correlation was analyzed between the two genes. The results showed that the beta-catenin gene in BMMNC of blast crisis of CML patients was expressed significantly higher than that in chronic phase (p < 0.001) and accelerated phase (p = 0.016) of CML patients and in normal donors (p = 0.004). The expression of bcr/abl in blast crisis of CML was statistically higher than that in chronic phase of CML (p = 0.001). The expression levels of beta-catenin and bcr/abl were correlated with each other in CML patients (r = 0.620, p < 0.001). It is concluded that the beta-catenin gene in blast crisis of CML patients express higher than that in chronic phase and accelerated phase of CML, and its expression level is correlated with the level of bcr/abl expression. The increased expression of beta-catenin may be account partly for the blast crisis of CML.
Li
Zeng-Jun
ZJ
State Key Laboratory of Experimental Hematology, Institute of Hematology, Blood Disease Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin 300020, China.
Qiu
Lu-Gui
LG
Li
Xin
X
Mai
Yu-Jie
YJ
Wang
Guo-Rong
GR
Yu
Zhen
Z
Wang
Ya-Fei
YF
Li
Chang-Hong
CH
Li
Qian
Q
D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000378:metabolism*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured; D055815:Young Adult; D051176:beta Catenin / Q000378:metabolism*
D051176:beta Catenin; D016044:Fusion Proteins, bcr-abl