title: Plant based insect repellent and insecticide treated bed nets to protect against malaria in areas of early evening biting vectors: double blind randomised placebo controlled clinical trial in the Bolivian Amazon.
abstract: OBJECTIVE
  To determine the effectiveness in reducing malaria of combining an insect repellent with insecticide treated bed nets compared with the nets alone in an area where vector mosquitoes feed in the early evening.
  
  
  METHODS
  A double blind, placebo controlled cluster-randomised clinical study.
  
  
  METHODS
  Rural villages and peri-urban districts in the Bolivian Amazon.
  
  
  METHODS
  4008 individuals in 860 households.
  
  
  METHODS
  All individuals slept under treated nets; one group also used a plant based insect repellent each evening, a second group used placebo.
  
  
  METHODS
  Episodes of Plasmodium falciparum or P vivax malaria confirmed by rapid diagnostic test or blood slide, respectively.
  
  
  RESULTS
  We analysed 15,174 person months at risk and found a highly significant 80% reduction in episodes of P vivax in the group that used treated nets and repellent (incidence rate ratio 0.20, 95% confidence interval 0.11 to 0.38, P<0.001). Numbers of P falciparum cases during the study were small and, after adjustment for age, an 82% protective effect was observed, although this was not significant (0.18, 0.02 to 1.40, P=0.10). Reported episodes of fever with any cause were reduced by 58% in the group that used repellent (0.42, 0.31 to 0.56, P<0.001).
  
  
  CONCLUSIONS
  Insect repellents can provide protection against malaria. In areas where vectors feed in the early evening, effectiveness of treated nets can be significantly increased by using repellent between dusk and bedtime. This has important implications in malaria vector control programmes outside Africa and shows that the combined use of treated nets and insect repellents, as advocated for most tourists travelling to high risk areas, is fully justified.
  
  
  BACKGROUND
  NCT 00144716.
authors.affiliation: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT. nigel.hill@lshtm.ac.uk
authors.forename: N
authors.initials: N
authors.lastname: Hill
authors.forename: A
authors.initials: A
authors.lastname: Lenglet
authors.forename: A M
authors.initials: AM
authors.lastname: Arnéz
authors.forename: I
authors.initials: I
authors.lastname: Carneiro
mesh_terms: D000818:Animals; D001512:Bedding and Linens; D001838:Bolivia; D004311:Double-Blind Method; D006801:Humans; D007302:Insect Repellents; D007303:Insect Vectors; D007306:Insecticides; D008288:Malaria / Q000517:prevention & control*; D009032:Mosquito Control / Q000379:methods*; D028321:Plant Preparations; D012423:Rural Health; D014504:Urban Health
chemical_list: D007302:Insect Repellents; D007306:Insecticides; D028321:Plant Preparations