O-glycan regulation of apoptosis and proliferation in colorectal cancer cell lines.
Cell growth pathways are mediated through protein-glycan interactions including O-glycosylation. Investigation of these growth pathways can be carried out using appropriate inhibitors to identify stage-specific events. We have adopted this approach to study a group of benzyl-O-N-acetyl-D-galactosamine analogues in human colorectal cancer cell lines. Exposure to O-glycan inhibitors resulted in the induction of apoptosis, a block in proliferation, accumulation of intracellular aryl-glycans and changes in related genes as detected by gene array. Colorectal cancer cell lines susceptible to the inhibitors showed growth arrest with all compounds. However, a differential action of each inhibitor was detected in the pattern of genes affected and in the structure of aryl-glycans formed.
Patsos
G
G
Mucin Research Group, Clinical Science@South Bristol, Bristol BS2 8HW, U.K.
Robbe-Masselot
C
C
Klein
A
A
Hebbe-Viton
V
V
Martin
R San
RS
Masselot
D
D
Graessmann
M
M
Paraskeva
C
C
Gallagher
T
T
Corfield
A
A
D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D006031:Glycosylation; D006801:Humans; D011134:Polysaccharides / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction
D011134:Polysaccharides