Resonance light scattering spectroscopy of beta-cyclodextrin-sodium dodecylsulfate-protein ternary system and its analytical applications.
A simple, highly sensitive and dye-less assay for proteins was reported using a resonance light-scattering (RLS) technique based on the enhanced RLS intensity of beta-cyclodextrin (beta-CD)-sodium dodecylsulfate (SDS)-protein system. Under the optimum conditions, the enhanced RLS intensity is in proportion to the concentration of proteins in the range of 0.01 to 2.3 microg ml(-1) for bovine serum albumin (BSA), 0.01 to 2.0 microg ml(-1) for human serum albumin (HSA), 0.015 to 5.0 microg ml(-1) for gamma-globulin (gamma-G), 0.02 to 3.5 microg ml(-1) for egg albumin (EA), 0.02 to 4.0 microg ml(-1) for pepsin (Pep), and 0.02 to 3.6 microg ml(-1) for alpha-chymotrypsin (Chy). Their detection limits (S/N = 3) are 1.1, 1.6, 2.4, 6.7, 5.4 and 4.2 ng ml(-1), respectively. Synthetic samples and human serum samples were determined satisfactorily, and the results were in reasonable agreement with those obtained by a documented spectrophotometric (Bradford) method.
Chen
Zhanguang
Z
Department of Chemistry, Shantou University, Shantou, P.R. China. lqlu@stu.edu.cn
Liu
Jinbin
J
Chen
Xiaoming
X
Zhang
Taiyu
T
Han
Yali
Y
D000818:Animals; D002021:Buffers; D002138:Calibration; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D018625:Microscopy, Atomic Force; D009994:Osmolar Concentration; D011506:Proteins / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods*; D013501:Surface-Active Agents / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000032:analysis*
D002021:Buffers; D011506:Proteins; D013501:Surface-Active Agents; D047392:beta-Cyclodextrins; D012967:Sodium Dodecyl Sulfate