Multivalent binding of small guest molecules and proteins to molecular printboards inside microchannels.
Beta-Cyclodextrin (beta-CD) monolayers have been immobilized in microchannels. The host-guest interactions on the beta-CD monolayers inside the channels were comparable to the interactions on beta-CD monolayers on planar surfaces, and a divalent fluorescent guest attached with a comparable binding strength. Proteins were attached to these monolayers inside microchannels in a selective manner by employing a strategy that uses streptavidin and orthogonal linker molecules. The design of the chip, which involved a large channel that splits into four smaller channels, allowed the channels to be addressed separately and led to the selective immobilization of antibodies. Experiments with labeled antibodies showed the selective immobilization of these antibodies in the separate channels.
Ludden
Manon J W
MJ
Molecular Nanofabrication group MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
Ling
Xing Yi
XY
Gang
Tian
T
Bula
Wojciech P
WP
Gardeniers
Han J G E
HJ
Reinhoudt
David N
DN
Huskens
Jurriaan
J
D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry; D014867:Water / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry
D011506:Proteins; D012235:Rhodamines; D047392:beta-Cyclodextrins; D014867:Water