title: Differential gene expression in nickel(II)-treated normal rat kidney cells.
abstract: Nickel(II) compounds are carcinogenic metals which induce genotoxicity and oxidative stress through the generation of reactive oxygen species. In search of new molecular pathways toward understanding the molecular mechanism of nickel(II)-induced carcinogensis, we performed mRNA differential display analysis using total RNA extracted from nickel(II) acetate-treated normal rat kidney cells (NRK-52E). Cells were exposed for 2 months to 160 and 240 microM nickel(II) concentrations. cDNAs corresponding to mRNAs for which expression levels were altered by nickel(II) were isolated, sequenced, and followed by a GenBank Blast homology search. Specificity of differential expression of cDNAs was determined by RT-PCR. Two of them (SH3BGRL3 and FHIT) were down-regulated and one (metallothionein) was up-regulated by nickel(II) treatment. The expression of these mRNAs were nickel(II) concentration-dependent. Overall, although the fundamental questions related to function of these genes in nickel(II)-mediated carcinogenicity are not answered, our study suggests that they can be interesting candidates for studies of molecular mechanisms of nickel(II) carcinogenesis.
authors.affiliation: Department of Biochemistry, College of Medicine, Soonchunhyang University, Cheon-An 330-090, Korea. L1037624@sch.ac.kr
authors.forename: Sang-Han
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000085:Acetates / Q000633:toxicity*; D017766:Acid Anhydride Hydrolases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000166:cytology; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009942:Organometallic Compounds / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013997:Time Factors; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D000085:Acetates; D017766:Acid Anhydride Hydrolases; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D008668:Metallothionein; D009363:Neoplasm Proteins; D009942:Organometallic Compounds; D012333:RNA, Messenger; D000099170:Bis(5'-adenosyl)triphosphatase; C119536:nickel acetate