title: Recipes for antimicrobial wine marinades against Bacillus cereus, Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella enterica.
abstract: We have evaluated bactericidal activities against Bacillus cereus, Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella enterica of several antimicrobial wine recipes, each consisting of red or white wine extracts of oregano leaves with added garlic juice and oregano oil. Dose-response plots were used to determine the percentage of the recipes that resulted in a 50% decrease in colony-forming units (CFU) at 60 min (BA(50)). Studies designed to optimize antibacterial activities of the recipes demonstrated that several combinations of the naturally occurring plant-derived ingredients rapidly inactivated the above mentioned 4 foodborne pathogens. We also showed that (a) incubation temperature affected activities in the following order: 37 degrees C > 21 degrees C > 4 degrees C; (b) varying the initial bacterial concentrations from 10(3) to 10(4) to 10(5) CFU/well did not significantly affect BA(50) values; (c) storage of 3 marinades up to 2 mo did not change their effectiveness against Salmonella enterica; and (d) polyphenolic compounds isolated by chromatography from red wine exhibited exceptional activity at nanogram levels against 2 strains of Bacillus cereus. These observations suggest that antimicrobial wine formulations have the potential to improve the microbiological safety of foods.
authors.affiliation: Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, Albany, CA 94710, USA. mfried@pw.usda.gov
authors.forename: Mendel
authors.initials: M
authors.lastname: Friedman
authors.forename: P R
authors.initials: PR
authors.lastname: Henika
authors.forename: C E
authors.initials: CE
authors.lastname: Levin
authors.forename: R E
authors.initials: RE
authors.lastname: Mandrell
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001409:Bacillus cereus / Q000187:drug effects* / Q000254:growth & development; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004305:Dose-Response Relationship, Drug; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D031341:Origanum / Q000737:chemistry; D019779:Salmonella enterica / Q000187:drug effects* / Q000254:growth & development; D013696:Temperature; D013997:Time Factors; D014920:Wine
chemical_list: D000900:Anti-Bacterial Agents