Intrapleural low-molecular-weight urokinase or tissue plasminogen activator versus single-chain urokinase in tetracycline-induced pleural loculation in rabbits.
The authors compared the ability of a single dose of the proenzyme single-chain urokinase (scuPA), low-molecular-weight urokinase, tissue plasminogen activator (tPA), or a mutant site-inactive scuPA to resolve intrapleural loculations at 72 to 96 hours after tetracycline-induced pleural injury in rabbits. Both scuPA and tPA reversed loculations at 96 hours after injury P < or = .001, whereas low-molecular-weight urokinase and the scuPA mutant were ineffective. scuPA and tPA generated inhibitor complexes, induced fibrinolytic activity, and quenched plasminogen activator-1 activity in pleural fluids. The authors conclude that scuPA reverses loculations as effectively as tPA at clinically applied intrapleural doses, whereas low-molecular-weight urokinase was ineffective.
Idell
Steven
S
Texas Lung Injury Institute, The University of Texas Health Science Center at Tyler, Tyler, Texas 75708, USA. steven.idell@uthct.edu
Azghani
Ali
A
Chen
Shande
S
Koenig
Kathy
K
Mazar
Andrew
A
Kodandapani
Lalitha
L
Bdeir
Khalil
K
Cines
Douglas
D
Kulikovskaya
Irina
I
Allen
Timothy
T
D000818:Animals; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D005343:Fibrinolytic Agents / Q000008:administration & dosage*; D010994:Pleura / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D010996:Pleural Effusion / Q000517:prevention & control; D010998:Pleurisy / Q000517:prevention & control; D018700:Pleurodesis; D011817:Rabbits; D013752:Tetracycline / Q000633:toxicity*; D000267:Tissue Adhesions / Q000517:prevention & control; D010959:Tissue Plasminogen Activator / Q000008:administration & dosage*; D014568:Urokinase-Type Plasminogen Activator / Q000008:administration & dosage*
D005338:Fibrin Fibrinogen Degradation Products; D005343:Fibrinolytic Agents; C036309:fibrin fragment D; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator; D013752:Tetracycline