Characterization and application of three novel monoclonal antibodies against human 4-1BB: distinct epitopes of human 4-1BB on lung tumor cells and immune cells.
4-1BB, a member of the tumor necrosis factor receptor (TNFR) superfamily, is a costimulatory receptor that is primarily expressed on activated T cells and professional antigen-presenting cells. In this study, the expression pattern of 4-1BB on immunology cells and tumor cells was explored by flow cytometry using newly generated three anti-4-1BB monoclonal antibodies (mAbs; 6F9, 7D6, and 1G11), which bind to distinct 4-1BB epitopes. Compared with the available 4-1BB mAb 4B4-1 that recognized 4-1BB on activated T cells and monocytes, the novel mAbs also could recognize 4-1BB on some cancer cell lines, particularly on lung cancer cell lines such as SPC-A-1, H446, H460, and H1299 by flow cytometry analysis, western blot, and RT-PCR. Immunohistochemistry staining showed the 4-1BB was expressed on lung tumor tissue (33/35) but not on normal lung tissue (3/3). It was determined that 4-1BB was strictly expressed on lung cancer cells, which may provide information on the 4-1BB signal in tumor immunology mechanism.
Zhang
G-B
GB
Clinical Immunology Laboratory, Suzhou University No. 1 Affiliated Hospital, Suzhou, China. smbxuegz@public1.sz.js.cn
Dong
Q-M
QM
Hou
J-Q
JQ
Ge
Y
Y
Ju
S-G
SG
Lu
B-F
BF
Zhang
X-G
XG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000378:metabolism*; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology; D015153:Blotting, Western; D002277:Carcinoma / Q000276:immunology*; D045744:Cell Line, Tumor; D000939:Epitopes / Q000032:analysis / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000032:analysis / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007962:Leukocytes / Q000145:classification / Q000276:immunology*; D008175:Lung Neoplasms / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9 / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000939:Epitopes; D018984:Epitopes, T-Lymphocyte; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9