title: Prospective exploration of biochemical tissue composition via imaging mass spectrometry guided by principal component analysis.
abstract: MALDI-based Imaging Mass Spectrometry (IMS) is an analytical technique that provides the opportunity to study the spatial distribution of biomolecules including proteins and peptides in organic tissue. IMS measures a large collection of mass spectra spread out over an organic tissue section and retains the absolute spatial location of these measurements for analysis and imaging. The classical approach to IMS imaging, producing univariate ion images, is not well suited as a first step in a prospective study where no a priori molecular target mass can be formulated. The main reasons for this are the size and the multivariate nature of IMS data. In this paper we describe the use of principal component analysis as a multivariate pre-analysis tool, to identify the major spatial and mass-related trends in the data and to guide further analysis downstream. First, a conceptual overview of principal component analysis for IMS is given. Then, we demonstrate the approach on an IMS data set collected from a transversal section of the spinal cord of a standard control rat.
authors.affiliation: Katholieke Universiteit Leuven, Department of Electrical Engineering (ESAT), SCD-SISTA (BIOI), Kasteelpark Arenberg 10, B-3001 Leuven (Heverlee), Belgium. raf.vandeplas@esat.kuleuven.be
authors.forename: Raf
authors.initials: R
authors.lastname: Van de Plas
authors.forename: Fabian
authors.initials: F
authors.lastname: Ojeda
authors.forename: Maarten
authors.initials: M
authors.lastname: Dewil
authors.forename: Ludo
authors.initials: L
authors.lastname: Van Den Bosch
authors.forename: Bart
authors.initials: B
authors.lastname: De Moor
authors.forename: Etienne
authors.initials: E
authors.lastname: Waelkens
mesh_terms: D000818:Animals; D019295:Computational Biology; D007091:Image Processing, Computer-Assisted / Q000706:statistics & numerical data*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D025341:Principal Component Analysis; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000706:statistics & numerical data*; D013116:Spinal Cord / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D009419:Nerve Tissue Proteins; D011506:Proteins