title: Importance of size-to-charge ratio in construction of stable and uniform nanoscale RNA/dendrimer complexes.
abstract: Formation of RNA/dendrimer complexes between various RNA molecules and PAMAM dendrimers was studied using atomic force microscopy. Our results demonstrate that effective construction of stable nanoscale and uniform RNA/dendrimer complexes depends critically on the size of the RNA molecule, the dendrimer generation and the charge ratio between the dendrimer and the RNA. Larger RNA molecules, higher generations of dendrimers and larger dendrimer-to-RNA charge ratios lead to the formation of stable, uniform nanoscale RNA/dendrimer complexes. These findings provide new insights in developing dendrimer systems for RNA delivery.
authors.affiliation: College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, P.R. China.
authors.forename: Xin-Cheng
authors.initials: XC
authors.lastname: Shen
authors.forename: Jiehua
authors.initials: J
authors.lastname: Zhou
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Liu
authors.forename: Jiangyu
authors.initials: J
authors.lastname: Wu
authors.forename: Fanqi
authors.initials: F
authors.lastname: Qu
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Zhang
authors.forename: Dai-Wen
authors.initials: DW
authors.lastname: Pang
authors.forename: Gilles
authors.initials: G
authors.lastname: Quéléver
authors.forename: Cheng-Cai
authors.initials: CC
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Peng
mesh_terms: D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D036103:Nanotechnology; D010316:Particle Size; D011073:Polyamines / Q000737:chemistry; D012313:RNA / Q000737:chemistry* / Q000648:ultrastructure; D020871:RNA Stability; D034741:RNA, Small Interfering / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D050091:Dendrimers; C104700:PAMAM Starburst; D011073:Polyamines; D034741:RNA, Small Interfering; D012313:RNA