Single-gene deletions that restore mating competence to diploid yeast.
Using the Saccharomyces cerevisiae MATa/MATalpha ORF deletion collection, homozygous deletion strains were identified that undergo mating with MATa or MATalpha haploids. Seven homozygous deletions were identified that confer enhanced mating. Three of these, lacking CTF8, CTF18, and DCC1, mate at a low frequency with either MATa or MATalpha haploids. The products of these genes form a complex involved in sister chromatid cohesion. Each of these strains also exhibits increased chromosome loss rates, and mating likely occurs due to loss of one copy of chromosome III, which bears the MAT locus. Three other homozygous diploid deletion strains, ylr193cDelta/ylr193cDelta, yor305wDelta/yor305wDelta, and ypr170cDelta/ypr170cDelta, mate at very low frequencies with haploids of either or both mating types. However, an ist3Delta/ist3Delta strain mates only with MATa haploids. It is shown that IST3, previously linked to splicing, is required for efficient processing of the MATa1 message, particularly the first intron. As a result, the ist3Delta/ist3Delta strain expresses unbalanced ratios of Matalpha to Mata proteins and therefore mates with MATa haploids. Accordingly, mating in this diploid can be repressed by introduction of a MATa1 cDNA. In summary, this study underscores and elaborates upon predicted pathways by which mutations restore mating function to yeast diploids and identifies new mutants warranting further study.
Schmidlin
Tom
T
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
Kaeberlein
Matt
M
Kudlow
Brian A
BA
MacKay
Vivian
V
Lockshon
Daniel
D
Kennedy
Brian K
BK
D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000502:physiology; D015825:Chromosomes, Fungal / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D004171:Diploidy; D017353:Gene Deletion; D049770:Genes, Mating Type, Fungal / Q000235:genetics / Q000502:physiology; D024101:Mitochondrial Proteins / Q000235:genetics / Q000502:physiology; D017413:Ribonucleoprotein, U2 Small Nuclear / Q000235:genetics / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology; D012854:Sister Chromatid Exchange / Q000235:genetics / Q000502:physiology
C092693:CTF18 protein, S cerevisiae; D002868:Chromosomal Proteins, Non-Histone; C429768:Ctf8 protein, S cerevisiae; D004268:DNA-Binding Proteins; C429769:Dcc1 protein, S cerevisiae; C425663:IST3 protein, S cerevisiae; D024101:Mitochondrial Proteins; D017413:Ribonucleoprotein, U2 Small Nuclear; D029701:Saccharomyces cerevisiae Proteins; C512497:Ups1 protein, S cerevisiae