title: 2,3,7,8-TCDD exposure, endothelial dysfunction and impaired microvascular reactivity.
abstract: Vascular function was examined in subjects with long-term high level of serum 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD) during their follow-up visits. Their earlier mean peak TCDD level at the time of exposure in 1965-1968 was estimated in the range of 3300-74 000 pg/g lipids. Ten former pesticide production workers heavily exposed to TCDD (age 57 +/- 2 years, TCDD about 170 pg/g lipids) were examined in 2001. Extended group of 15 TCDD-exposed men (age 59 +/- 3 years, TCDD about 130 pg/g lipids) underwent the same examination in 2004. Findings were compared with a control group of 14 healthy men (age 54 +/- 2 years). Skin microvascular reactivity (MVR) was measured by laser Doppler perfusion monitoring in the forearm during post-occlusive reactive hyperemia (PORH) and thermal hyperemia (TH). Several parameters of MVR in men exposed to TCDD were significantly impaired, compared with the control group and further progression of the impairment of MVR has been observed between years 2001 and 2004. Serum concentration of E-selectin and inhibitor of tissue plasminogen activator 1 (PAI-1) was significantly higher in exposed subjects (56.0 +/- 18.4 ng/mL versus 40.0 +/- 12.0 ng/mL, P = 0.022 and 90.9 +/- 33.3 ng/mL versus 45.0 +/- 18.0, P = 0.002, respectively). In addition, PORH in the forearm was significantly negatively associated with SOD activity (r = -0.77, P = 0.009) as well as the velocity of perfusion increase during TH (r = -0.68, P = 0.03) and TH% (r = -0.78, P = 0.008). Our data document the presence of endothelial dysfunction in TCDD-exposed men.
authors.affiliation: Department of Occupational Medicine, 1st Medical Faculty, Charles University and General University Hospital, Na Bojisti 1, Prague, Czech Republic. daniela.pelclova@lf1.cuni.cz
authors.forename: Daniela
authors.initials: D
authors.lastname: Pelclová
authors.forename: Martin
authors.initials: M
authors.lastname: Prázny
authors.forename: Jan
authors.initials: J
authors.lastname: Skrha
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Fenclová
authors.forename: Marta
authors.initials: M
authors.lastname: Kalousová
authors.forename: Pavel
authors.initials: P
authors.lastname: Urban
authors.forename: Tomás
authors.initials: T
authors.lastname: Navrátil
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Senholdová
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Smerhovsky
mesh_terms: D000368:Aged; D001783:Blood Flow Velocity / Q000187:drug effects; D002318:Cardiovascular Diseases / Q000097:blood / Q000139:chemically induced* / Q000503:physiopathology; D019040:E-Selectin / Q000097:blood; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000503:physiopathology; D005500:Follow-Up Studies; D005542:Forearm / Q000098:blood supply*; D006801:Humans; D006940:Hyperemia / Q000503:physiopathology; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D017078:Laser-Doppler Flowmetry; D008055:Lipids / Q000097:blood; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D009784:Occupational Diseases / Q000097:blood / Q000139:chemically induced* / Q000503:physiopathology; D016273:Occupational Exposure; D010575:Pesticides / Q000097:blood / Q000633:toxicity*; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D000072317:Polychlorinated Dibenzodioxins / Q000097:blood / Q000633:toxicity*; D012039:Regional Blood Flow / Q000187:drug effects; D012867:Skin / Q000098:blood supply*; D013482:Superoxide Dismutase / Q000097:blood; D013997:Time Factors
chemical_list: D019040:E-Selectin; D008055:Lipids; D010575:Pesticides; D017395:Plasminogen Activator Inhibitor 1; D000072317:Polychlorinated Dibenzodioxins; C051321:SERPINE1 protein, human; D018799:Intercellular Adhesion Molecule-1; D013482:Superoxide Dismutase