Oxidative stress-induced leaky sarcoplasmic reticulum underlying acute heart failure in severe burn trauma.
Burn trauma causes cardiac dysfunction. However, much of the underlying cellular and molecular mechanisms remain elusive. In the present study, we demonstrate the roles of excessive sarcoplasmic reticulum (SR) Ca(2+) leakage and oxidative stress in burn-associated acute heart failure. In cardiomyocytes from failing rat hearts 12 h after full-thickness cutaneous burn of about 40% of the total body surface area, we found that Ca(2+) transients and contractility were impaired, but the triggering L-type Ca(2+) channel current density was unaltered, giving rise to a significantly reduced gain of excitation-contraction coupling. This deficiency in SR Ca(2+) release was accompanied by a reduction in Ca(2+) content in the SR. Surprisingly, the frequency of spontaneous Ca(2+) sparks was increased by 1.4-fold; Ca(2+) tolerance test (10 mM extracellular Ca(2+)) further showed 2.0- and 1.5-fold more frequent Ca(2+) waves and Ca(2+) sparks, respectively. Myofilament sensitivity to Ca(2+), however, seemed to be unaffected. These results suggest hyperactivity of the ryanodine receptor (RyR) Ca(2+) release channel and a leaky SR in burn. Importantly, pretreatment with antioxidant vitamins C and E seemed to prevent burn-induced RyR hypersensitivity and SR leakage and thereby normalize Ca(2+) transients and contractility. Concomitantly, the in vivo cardiac functions were also more tolerant of traumatic burn. Collectively, our findings suggest that SR leakage due to oxidative stress is likely a major candidate mechanism underlying burn-associated acute heart failure. Antioxidant therapy in burn trauma provides cardioprotection, at least in part, by protecting RyR's from oxidative stress-induced hypersensitivity.
Deng
Jianxin
J
Department of Pathophysiology, Southern Medical University, Guangzhou 510515, China.
Wang
Gang
G
Huang
Qiaobing
Q
Yan
Yuan
Y
Li
Kaitao
K
Tan
Wenchang
W
Jin
Chunhua
C
Wang
Yanru
Y
Liu
Jie
J
D000200:Action Potentials; D000208:Acute Disease; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D002056:Burns / Q000150:complications*; D002118:Calcium / Q000378:metabolism*; D005260:Female; D006333:Heart Failure / Q000209:etiology* / Q000378:metabolism / Q000517:prevention & control; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
D000975:Antioxidants; D019837:Ryanodine Receptor Calcium Release Channel; D002118:Calcium