[Effect of schistosome ova on Trinitrobenzenesulfonic acid induced colitis in mice].
OBJECTIVE
To investigate the effects of intraperitoneal injected schistosome ova on TNBS-induced colitis and on the intestinal TLR4 expression in mice.


METHODS
40 BALB/c mice were randomized into 3 groups: normal control group (10 mice), TNBS group (20 mice) in which mice were exposed to trinitrobenzesulfonic acid (TNBS) and were induced with colitis, and the schistosome ova group (10 mice) in which mice were intraperitoneal injected with freeze-killed schistosome ova and later exposed to TNBS. The following variables were observed: mortality, pathological appearance of the colon, histological scoring of the specimen, serum TNF-alpha level, and intestinal TLR4 expression detected by RT-PCR and Immunohistochemistry.


RESULTS
Mortality of schistosome ova group was lower than that of the TNBS group (20% vs 70%, P < 0.05). Inflammation of the mice colon in the schistosome ova group was less severe than that of the TNBS group (1.4 +/- 0.5 vs 4.2 +/- 0.6, P < 0.01, Ameho criteria scoring). TLR4 expression of colon was up-regulated in mice of TNBS group and down-regulated in schistosome ova group which was still higher than that of normal controls (0.762 +/- 0.054 vs 0.325 +/- 0.029 vs 0.237 +/- 0.021, P < 0.01).


CONCLUSIONS
Intraperitoneal injected schistosome ova can obviously reduce TNBS-induced colitis in mice, which may be attributed to down-regulated TLR4 expression in colon.
Jiang
Jie
J
Department of Gastroenterology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Xue
Ru-yi
RY
Zhang
Shun-cai
SC
Zhou
Jun
J
Zhou
Kang
K
D000818:Animals; D003106:Colon / Q000378:metabolism / Q000469:parasitology / Q000473:pathology; D007150:Immunohistochemistry; D015212:Inflammatory Bowel Diseases / Q000139:chemically induced / Q000469:parasitology* / Q000517:prevention & control; D007274:Injections, Intraperitoneal; D007413:Intestinal Mucosa / Q000378:metabolism / Q000469:parasitology* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010063:Ovum / Q000502:physiology*; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012549:Schistosoma japonicum; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis / Q000235:genetics; D014302:Trinitrobenzenesulfonic Acid
D051197:Toll-Like Receptor 4; D014302:Trinitrobenzenesulfonic Acid