Quantification of in vivo tumor invasion and vascularization by computerized image analysis.
The matrix-inserted surface transplantation model is an in vivo assay used to analyse the kinetics of tumor-vessel interactions during different stages of skin carcinoma progression. This system allows the study of host-tumor interface, i.e. penetration of tumor cells into normal host tissue as well as infiltration of normal host cells into the tumor. In the present study, image analysis algorithms for processing and quantifying the extent of such migratory and tissue remodeling events are presented. The proposed method is non-parametric and its originality lies in its particularity to take into account the specific geometry of tumor-host interface. This methodology is validated by evaluating the contribution of matrix metalloproteases (MMPs) in skin carcinoma invasion and vascularization through pharmacological and genetic approaches.
Blacher
S
S
Laboratory of Tumor and Development Biology, Centre de Recherche en Cancérologie Expérimentale, Groupe Interdisciplinaire de Génoprotéomique Apliquée, University of Liège, Tour de Pathologie, CHU (B23), Sart-Tilman, Liege, Belgium. silvia.blacher@ulg.ac.be
Jost
M
M
Melen-Lamalle
L
L
Lund
L R
LR
Romer
J
J
Foidart
J M
JM
Noël
A
A
D000465:Algorithms; D020533:Angiogenesis Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D004151:Dipeptides / Q000494:pharmacology / Q000627:therapeutic use; D007700:Kinetics; D053505:Matrix Metalloproteinases, Secreted / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015233:Models, Statistical; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic / Q000201:enzymology / Q000473:pathology* / Q000517:prevention & control; D011480:Protease Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted; D012878:Skin Neoplasms / Q000098:blood supply* / Q000201:enzymology / Q000473:pathology* / Q000517:prevention & control; D017154:Stromal Cells / Q000473:pathology
D020533:Angiogenesis Inhibitors; D004151:Dipeptides; C078131:N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide; D011480:Protease Inhibitors; D053505:Matrix Metalloproteinases, Secreted; C108362:matrix metalloproteinase 19