title: Synthesis and cytotoxicity evaluation of 22,23-oxygenated stigmastane derivatives.
abstract: Starting from (22E)-3alpha,5alpha-cyclo-6beta-methoxystigmast-22-ene eighteen derivatives of (22S,23S)-22,23-oxidostigmastane, (22R,23R)-22,23-oxidostigmastane, and (22R,23R)-22,23-dihydroxystigmastane were synthesized and screened for cytotoxicity in human hepatoma Hep G2 cells and human breast carcinoma MCF-7 cells using MTT assay. Four compounds of this series exhibited high cytotoxicity in both cells; three compounds were selectively toxic in MCF-7 cells, one compound was toxic in Hep G2 cells, rather than in MCF-7 cells; four compounds at low concentrations increased MTT test values over the control.
authors.affiliation: V.N. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Alexander Yu
authors.initials: AY
authors.lastname: Misharin
authors.forename: Arif R
authors.initials: AR
authors.lastname: Mehtiev
authors.forename: Galina E
authors.initials: GE
authors.lastname: Morozevich
authors.forename: Yaroslav V
authors.initials: YV
authors.lastname: Tkachev
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Timofeev
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002783:Cholestenones / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity*; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D010100:Oxygen / Q000737:chemistry*; D011522:Protons; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
chemical_list: D002783:Cholestenones; D011522:Protons; D010100:Oxygen