In vivo cerebral artery microangiography in rat and mouse using synchrotron radiation imaging system.
Microangiography with spatial resolution in the micrometer range was carried out to depict vascular responses of the cerebral artery and arterioles in rats and mice using a real-time imaging system and a third generation synchrotron radiation source at SPring-8. An X-ray direct-conversion type detector with 6 microm spatial resolution was developed for real-time biomedical imaging. The X-ray image is converted directly into an electrical signal in the photoconductive layer without image blurring. In synchrotron radiation radiography, a long source-to-object distance and a small source spot can produce high-resolution images. Microangiographic images were obtained without image blurring and were stored in a digital frame memory system with a 1024 x 1024-pixel, 10-bit format. In imaging experiments, vasoconstriction and vasodilatation of small cerebral arteries were visualized in response to hypercapnia, hemorrhagic hypotension, and vasoactive agents after iodine contrast agent injection into the carotid artery.
Umetani
Keiji
K
Japan Synchrotron Radiation Research Institute, Sayo-Gun, Hyogo, Japan. umetani@spring8.or.jp
Kidoguchi
Keiji
K
Morishita
Akitsugu
A
Oizumi
Ximena-Sayuri
XS
Tamaki
Masahiro
M
Yamashita
Haruo
H
Sakurai
Takashi
T
Kondoh
Takeshi
T
D000792:Angiography / Q000379:methods*; D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000000981:diagnostic imaging*; D002339:Carotid Arteries / Q000000981:diagnostic imaging; D002560:Cerebrovascular Circulation / Q000187:drug effects; D044962:Data Compression / Q000379:methods; D006470:Hemorrhage / Q000000981:diagnostic imaging; D006935:Hypercapnia / Q000000981:diagnostic imaging; D007022:Hypotension / Q000000981:diagnostic imaging; D051379:Mice; D051381:Rats; D017356:Synchrotrons; D014661:Vasoconstriction / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000494:pharmacology
D014662:Vasoconstrictor Agents