[Metabolism characteristics of Acinetobacter sp. IMS B-7005 grown on the mixture of C2,-C6-substrates].
It has been established that under the conditions of mixotrophic growth of Acinetobacter sp. B-7005 on the mixture of sodium acetate and glucose the activity of acetyl-KoA-synthetase and that of phosphoenolpyruvate synthetase were 10 times lower and indices of synthesis of microbial exopolysaccharide (EPS) ethapolan are almost 2 times lower than on the mixture of potassium acetate and glucose. It is supposed that the positive role of sodium cations may consist in their participation in the active transport of acetate to the cells of Acinetobacter sp. B-7005. An increase of ethapolan synthesis when the producer is grown on the mixture of energy-deficient (acetate + glucose) and energetically nonequivalent (ethanol + glucose) substrates is determined by the intensification of gluconeogenesis, that is evidenced by the functioning of two anaplerotic paths (glioxylate cycle and pyruvate-carboxylase reaction), as well as the increase of activity of phosphoenolpyruvate synthetase compared with cultivation of bacteria on the corresponding monosubstrates.
Pyroh
T P
TP
Korzh
Iu V
IuV
Vysiatets'ka
N V
NV
D000106:Acetate-CoA Ligase / Q000378:metabolism; D000150:Acinetobacter / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D017866:Phosphotransferases (Paired Acceptors) / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D019346:Sodium Acetate / Q000737:chemistry*; D013379:Substrate Specificity
D003470:Culture Media; D011135:Polysaccharides, Bacterial; D000431:Ethanol; D019346:Sodium Acetate; D017866:Phosphotransferases (Paired Acceptors); C022509:pyruvate, water dikinase; D000106:Acetate-CoA Ligase; D005947:Glucose