title: Membrane-bound conformation of peptaibols with methyl-deuterated alpha-amino isobutyric acids by 2H magic angle spinning solid-state NMR spectroscopy.
abstract: We present the use of 2H magic-angle spinning (MAS) NMR on methyl-deuterated alpha-amino isobutyric acid (Aib) as a new method to obtain fast and accurate structural constraints on peptaibols in membrane-bound environments. Using nonoriented vesicle-reconstituted samples we avoid the delicate preparation of oriented samples, and the use of MAS ensures high sensitivity and thereby very fast acquisition of experimental spectra. Furthermore, given the high content ( approximately 40%) of Aib in peptaibols and the fact that the amino acid Aib may be synthesized from cheap starting materials, even in the case of 2H, 13C, or 15N labeling, this method is ideally suited for studies of the membrane-bound conformation of peptaibols.
authors.affiliation: Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, University of Aarhus, Langelandsgade 140, DK-8000 Aarhus C, Denmark.
authors.forename: Kresten
authors.initials: K
authors.lastname: Bertelsen
authors.forename: Jan M
authors.initials: JM
authors.lastname: Pedersen
authors.forename: Brian S
authors.initials: BS
authors.lastname: Rasmussen
authors.forename: Troels
authors.initials: T
authors.lastname: Skrydstrup
authors.forename: Niels Chr
authors.initials: NC
authors.lastname: Nielsen
authors.forename: Thomas
authors.initials: T
authors.lastname: Vosegaard
mesh_terms: D000621:Aminoisobutyric Acids / Q000737:chemistry*; D003903:Deuterium / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008958:Models, Molecular; D008968:Molecular Conformation; D054848:Peptaibols / Q000737:chemistry*
chemical_list: D000621:Aminoisobutyric Acids; D054848:Peptaibols; D003903:Deuterium