title: A novel cryptic peptide derived from the rat neuropeptide FF precursor reverses antinociception and conditioned place preference induced by morphine.
abstract: Neuropeptide FF (NPFF) precursors from different species contain at least three known neuropeptides, i.e. FF (FLFQPQRF-NH(2)), AF (AGEGLSSPFWSLAAPQR-NH(2)) and SF (SLAAPQRF-NH(2)). We demonstrate that the rat NPFF precursor contains another bioactive sequence, NAWGPWSKEQLSPQA, spanning between positions 85 and 99. Synthetic NPFF precursor (85-99) (10 and 20 nmol, i.c.v.) blocked the expression of conditioned place preference induced by morphine (5 mg/kg, s.c.). This peptide alone (10 and 20 nmol, i.c.v.) had no influence on the baseline latency of a nociceptive reaction but reversed the antinociceptive activity of morphine (5 mg/kg, s.c.) in the tail-immersion test in rats. These data suggest the existence of a novel bioactive cryptic peptide within an already known NPFF precursor.
authors.affiliation: Faculty of Chemistry and Regional Laboratory, Jagiellonian University, Ingardena 3, Krakow, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Dylag
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Pachuta
authors.forename: Hana
authors.initials: H
authors.lastname: Raoof
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kotlinska
authors.forename: Jerzy
authors.initials: J
authors.lastname: Silberring
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003216:Conditioning, Operant / Q000187:drug effects*; D008297:Male; D008969:Molecular Sequence Data; D009020:Morphine / Q000494:pharmacology*; D009619:Nociceptors / Q000187:drug effects / Q000503:physiopathology; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017386:Sequence Homology, Amino Acid
chemical_list: D009842:Oligopeptides; D010455:Peptides; D009020:Morphine; C053651:phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide