Toxoplasma gondii: detection of MIC10 antigen in sera of experimentally infected mice.
We developed a sandwich ELISA for the detection of circulating Toxoplasma gondii MIC10 antigens. In T. gondii culture supernatant, MIC10 was detected in a growth dependent manner. Mice were infected with a lethal dose of either a virulent RH strain, an avirulent Beverley strain or a sub-lethal dose of a PLK strain of T. gondii. MIC10 appeared 2 days after infection and increased gradually in the sera of RH-infected mice. A detectable but significantly lower amount of MIC10 was observed in the sera of mice infected intraperitoneally with Beverley tachyzoites. In contrast, the MIC10 antigen in mice sera following oral infection with Beverley cysts was below detectable levels during the course of the experiment. In sera of PLK-infected mice, MIC10 was predominantly observed between late acute and early chronic phase. Our data show that the kinetics of circulating MIC10 differs depending on the strain and route of infection.
Dautu
George
G
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, 080-8555 Japan.
Ueno
Akio
A
Miranda
Aracelis
A
Mwanyumba
Sophie
S
Munyaka
Biscah
B
Carmen
Gabriella
G
Kariya
Tatsuya
T
Omata
Yoshitaka
Y
Saito
Atsushi
A
Xuan
Xuenan
X
Igarashi
Makoto
M
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis / Q000276:immunology; D000953:Antigens, Protozoan / Q000097:blood* / Q000235:genetics; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D020224:Expressed Sequence Tags / Q000737:chemistry; D005260:Female; D020869:Gene Expression Profiling; D007700:Kinetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins / Q000097:blood / Q000235:genetics; D011817:Rabbits; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D014124:Toxoplasmosis, Animal / Q000175:diagnosis / Q000276:immunology* / Q000469:parasitology; D014774:Virulence
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins