Actinomycetemcomitin: a new bacteriocin produced by Aggregatibacter (Actinobacillus) actinomycetemcomitans.
Aggregatibacter (Actinobacillus) actinomycetemcomitans P(7-20) strain isolated from a periodontally diseased patient has produced a bacteriocin (named as actinomycetemcomitin) that is active against Peptostreptococcus anaerobius ATCC 27337. Actinomycetemcomitin was produced during exponential and stationary growth phases, and its amount decreased until it disappeared during the decline growth phase. It was purified by ammonium sulphate precipitation (30-60% saturation), and further by FPLC (mono-Q ionic exchange and Phenyl Superose hydrophobic interaction) and HPLC (C-18 reversed-phase). This bacteriocin loses its activity after incubation at a pH below 7.0 or above 8.0, following heating for 30 min at 45 degrees C, and after treatment with proteolytic enzymes such as trypsin, alpha-chymotrypsin, and papain. Actinomycetemcomitin has a molecular mass of 20.3 KDa and it represents a new bacteriocin from A. actinomycetemcomitans.
Lima
Francisca Lúcia
FL
Instituto de Microbiologia Prof. Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
de Carvalho
Maria Auxiliadora Roque
MA
Apolônio
Ana Carolina Morais
AC
Bemquerer
Marcelo Porto
MP
Santoro
Marcelo Matos
MM
Oliveira
Jamil Silvano
JS
Alviano
Celuta Sales
CS
Farias
Luiz de Macêdo
Lde M
D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D000645:Ammonium Sulfate; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D005590:Fractional Precipitation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism; D010462:Peptostreptococcus / Q000187:drug effects; D010518:Periodontitis / Q000382:microbiology; D016415:Sequence Alignment; D013696:Temperature
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010447:Peptide Hydrolases; D000645:Ammonium Sulfate