SK-PC-B70M from Pulsatilla koreana improves scopolamine-induced impairments of memory consolidation and spatial working memory.
Previous studies have shown that hederacolchiside-E from Pulsatilla koreana has neuroprotective effects and cognition-enhancing effects. Subsequently, in the current study, we demonstrate that oral administrations of oleanolic-glycoside saponins enriched fraction from P. koreana, designated as SK-PC-B70M, improve impairments in memory consolidation and spatial working memory by systemic injection of scopolamine, a muscarinic cholinergic receptor antagonist. In a step-through avoidance task, when the rats stepped through a dark chamber in a shuttle box, an electric shock was given and then SK-PC-B70M was administered 30 min later. Twenty-four hours later, the rats were placed in an illuminated chamber. The rats with SK-PC-B70M treatments showed longer response latencies than rats with only scopolamine. Spatial working memory was measured with a trial-unique matching-to-place task in a water maze which assessed memory for place information over varying lengths of delays. Three delay lengths were used: 1 min, 5 min, and 3 h. In comparison with the control rats, the rats with scopolamine treatments took significantly longer to find the platform in the second trial with 1- and 5-min delays. The rats with both scopolamine and SK-PC-B70M had significantly less search error compared with the rats with scopolamine only. These findings indicate that SK-PC-B70M has effects on reversing impairments of memory consolidation and working memory impairments induced by scopolamine.
Han
Chang-Kyun
CK
Life Science R&D Center, SK Chemicals, Suwon, Gyeonggi-Do, South Korea.
Park
Yang Hae
YH
Jin
Da-Qing
DQ
Hwang
Yoo Kyeong
YK
Oh
Ki-Bong
KB
Han
Jung-Soo
JS
D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D001522:Behavior, Animal / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008569:Memory Disorders / Q000139:chemically induced / Q000188:drug therapy*; D008570:Memory, Short-Term / Q000187:drug effects*; D008517:Phytotherapy / Q000379:methods*; D028321:Plant Preparations / Q000008:administration & dosage*; D031946:Pulsatilla / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects; D012503:Saponins / Q000627:therapeutic use; D012601:Scopolamine
D028321:Plant Preparations; C527801:SK-PC-B70M; D012503:Saponins; D012601:Scopolamine