title: Local tissue reaction and differentiation of the prosthesis-specific antibody response following functional implantation of vascular grafts in pigs.
abstract: The study was aimed at investigating the local tissue reactions through a histological examination of beta-1-integrin expression and neointima formation, and humoral immune responses by detection of prosthesis-specific antibodies, after functional implantation of vascular prostheses. In three groups of pigs, the infrarenal aorta was replaced with segments of collagen-impregnated Dacron-prostheses: M-prosthesis--medium primary porosity, double velour layer, crimping; C-prosthesis--low primary porosity, no velour, crimping; T-prosthesis--high primary porosity, no velour, no crimping. For each prosthesis type, one series with four animals was used for examining the tissue reactions, and a second series with eight animals was used to study the antibody response, both until 116 days postoperative. In the first three weeks, the M-prosthesis caused the highest tissue reactions and the highest antipolyester antibodies, but the lowest anticollagen antibodies, whereas the T-prosthesis caused the highest anticollagen antibodies, but the least tissue response and antipolyester antibodies in the early phase. On day 116, the C-prosthesis caused the highest tissue reactions and highest polyester-specific antibodies. These results indicate a possible association between local inflammatory reactions and humoral immune responses, influenced by properties of vascular grafts.
authors.affiliation: Department of Surgery, Hospital Riesa/Grossenhain, Riesa, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Zippel
authors.forename: Lutz
authors.initials: L
authors.lastname: Wilhelm
authors.forename: Andreas
authors.initials: A
authors.lastname: Hoene
authors.forename: Uwe
authors.initials: U
authors.lastname: Walschus
authors.forename: Torsten
authors.initials: T
authors.lastname: Ueberrueck
authors.forename: Michael
authors.initials: M
authors.lastname: Schlosser
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D001011:Aorta / Q000276:immunology / Q000473:pathology; D001807:Blood Vessel Prosthesis / Q000009:adverse effects*; D002454:Cell Differentiation / Q000276:immunology; D003094:Collagen / Q000276:immunology; D005260:Female; D007249:Inflammation / Q000209:etiology / Q000276:immunology / Q000473:pathology; D019012:Integrin beta1 / Q000276:immunology*; D008422:Materials Testing / Q000379:methods; D011091:Polyesters / Q000009:adverse effects; D016062:Porosity; D013552:Swine; D013997:Time Factors; D017539:Tunica Intima / Q000276:immunology* / Q000473:pathology
chemical_list: D000906:Antibodies; D019012:Integrin beta1; D011091:Polyesters; D003094:Collagen