Construction of in situ oligonucleotide arrays on plastic.
The concept of DNA arrays was first introduced in the early 1980s, by Sir Edwin Southern. Since then, many research institutions and biotechnology companies have investigated the potential use of arrays in fields ranging from genetic diagnostics to forensics investigations. A 64-channel automated chemical delivery system, known as the Southern Array Maker, which synthesizes oligonucleotides directly onto an aminated polypropylene substrate has been constructed. Many different arrays have been synthesized for the purpose of detecting single point mutations, which might be either indicators of, or directly responsible for, many different types of genetic diseases and cancers. These include cystic fibrosis, H-ras, K-ras, and other mutations. In addition to the synthesized arrays, we are also looking into various alternative methods of producing both high-and low-density DNA arrays. This chapter is intended to demonstrate the synthesis of oligoarrays by in situ method using standard phosphoramidite chemistry. Phosphoramidate linkage to the aminated polypropylene is quite stable under oligo cleavage and deprotection conditions. Oligonucleotide density is approx 3 pmole or 10(1)2 molecules/mm(2).
Rampal
Jang B
JB
Beckman Coulter, Inc., Brea, CA, USA.
Coassin
Peter J
PJ
Matson
Robert S
RS
D000588:Amines; D003550:Cystic Fibrosis / Q000235:genetics; D015342:DNA Probes / Q000378:metabolism; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009841:Oligonucleotides / Q000378:metabolism; D010969:Plastics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011126:Polypropylenes; D012984:Software
D000588:Amines; D015342:DNA Probes; D009841:Oligonucleotides; D010969:Plastics; D011126:Polypropylenes