Expression of stress-response proteins upon whitefly-mediated inoculation of Tomato yellow leaf curl virus in susceptible and resistant tomato plants.
To better understand the nature of resistance of tomato to the whitefly (Bemisia tabaci, B biotype)-transmitted Tomato yellow leaf curl virus (TYLCV), whiteflies and TYLCV were considered as particular cases of biotic stresses and virus resistance as a particular case of successful response to these stresses. Two inbred tomato lines issued from the same breeding program that used Solanum habrochaites as a TYLCV resistance source, one susceptible and the other resistant, were used to compare the expression of key proteins involved at different stages of the plant response with stresses: mitogen-activated protein kinases (MAPKs), cellular heat shock proteins (HSPs, proteases), and pathogenesis-related (PR) proteins. The two biotic stresses-non-viruliferous whitefly feeding and virus infection with viruliferous insects--led to a slow decline in abundance of MAPKs, HSPs, and chloroplast protease FtsH (but not chloroplast protease ClpC), and induced the activities of the PR proteins, beta-1,3-glucanase, and peroxidase. This decline was less pronounced in virus-resistant than in virus-susceptible lines. Contrary to whitefly infestation and virus infection, inoculation with the fungus Sclerotinia sclerotiorum induced a rapid accumulation of the stress proteins studied, followed by a decline; the virus-susceptible and -resistant tomato lines behaved similarly in response to the fungus.
Gorovits
Rena
R
Institute of Plant Sciences and Genetics in Agriculture and the Otto Warburg Minerva Center for Agricultural Biotechnology, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
Akad
Fouad
F
Beery
Hila
H
Vidavsky
Favi
F
Mahadav
Assaf
A
Czosnek
Henryk
H
D000818:Animals; D002688:Chitinases / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005959:Glucosidases / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D006430:Hemiptera / Q000502:physiology* / Q000821:virology; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism* / Q000469:parasitology / Q000821:virology*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D010935:Plant Diseases / Q000821:virology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010942:Plant Viruses / Q000502:physiology*
D006360:Heat-Shock Proteins; D010940:Plant Proteins; D010544:Peroxidases; D048049:Extracellular Signal-Regulated MAP Kinases; D020929:Mitogen-Activated Protein Kinase Kinases; D005959:Glucosidases; D002688:Chitinases