title: A 2-step cooking method of searing and hot water pasteurization to maximize the safety of refrigerated, vacuum packaged, chicken breast meat.
abstract: Americans consume almost 40 kg per capita of chicken each year. Increasing consumption of chicken surpassed pork in 1982 and beef in 1992. The objectives of this study were to examine the effectiveness of a novel, 2-step cooking method of grilling, slicing, vacuum packaging, and hot water pasteurization to inhibit the growth of Listeria monocytogenes in chicken breast meat. Because this study required the use of pilot plant scale pasteurization equipment, Listeria innocua M1, a nonpathogen with slightly greater heat resistance than L. monocytogenes, was used as a surrogate. We first examined the lethal effects of grilling on a boneless skinless chicken breast to mimic cross-contaminated, surface-inoculated Listeria. Searing produced a mean reduction of 2.5 log CFU/g of Listeria and a moisture loss of only 7% (w/w). A 2nd experiment studied the lethal effect of pasteurization of the sliced seared chicken breast. L. innocua M1 inoculated between the slices mimicked contamination in deep muscle. Pasteurization in a 71 degrees C bath (final internal temperature of 66 degrees C) gave an additional 2.3 log CFU/g reduction. L. innocua M1 did not show significant regrowth during a wk of refrigerated storage. The combined 2-step cooking method of searing and pasteurization gave a combined 4.8 log reduction in LI M1. In parallel tests a non-Listeria indicator, Corynebacterium glutamicum, inoculated between sliced, seared chicken, showed a 3 log reduction after pasteurization for 10 min in a 71 degrees C bath compared to 2.3 log reduction of Listeria. Corynebacterium regrowth occurred much faster than did L. innocua M1.
authors.affiliation: Dept. of Food Science, Univ. of Arkansas, Fayetteville, AR 72701, USA.
authors.forename: D K
authors.initials: DK
authors.lastname: Enns
authors.forename: P G
authors.initials: PG
authors.lastname: Crandall
authors.forename: C A
authors.initials: CA
authors.lastname: O'Bryan
authors.forename: C L
authors.initials: CL
authors.lastname: Griffis
authors.forename: E M
authors.initials: EM
authors.lastname: Martin
mesh_terms: D000818:Animals; D002645:Chickens / Q000382:microbiology*; D015169:Colony Count, Microbial; D003296:Cooking / Q000379:methods*; D048230:Corynebacterium glutamicum / Q000254:growth & development; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D018857:Food Packaging / Q000379:methods*; D006358:Hot Temperature; D008087:Listeria / Q000254:growth & development; D008089:Listeria monocytogenes / Q000254:growth & development; D012034:Refrigeration; D013696:Temperature; D014618:Vacuum; D014867:Water
chemical_list: D014867:Water