title: Metabolism of reactive oxygen species is attenuated in leghemoglobin-deficient nodules of Lotus japonicus.
abstract: Leghemoglobins together with high rates of respiration are believed to be major sources of reactive oxygen species (ROS) in root nodules of leguminous plants. High capacities of antioxidative systems apparently protect this organ from oxidative damage. Using leghemoglobin-RNA interference (LbRNAi) lines of Lotus japonicus, we found that loss of leghemoglobin results in significantly lower H(2)O(2) levels in nodules. Transcript levels and catalytic activities of ascorbate-glutathione cycle enzymes involved in H(2)O(2) detoxification as well as concentrations of reduced ascorbate were also altered in LbRNAi nodules. Thus, symbiotic leghemoglobins contribute significantly to ROS generation in functional nodules.
authors.affiliation: Max-Planck-Institute of Molecular Plant Physiology, Golm, Germany.
authors.forename: Catrin
authors.initials: C
authors.lastname: Günther
authors.forename: Armin
authors.initials: A
authors.lastname: Schlereth
authors.forename: Michael
authors.initials: M
authors.lastname: Udvardi
authors.forename: Thomas
authors.initials: T
authors.lastname: Ott
mesh_terms: D060387:Ascorbate Peroxidases; D001205:Ascorbic Acid / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007874:Leghemoglobin / Q000037:antagonists & inhibitors*; D000070116:Lotus / Q000254:growth & development / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D053204:Root Nodules, Plant / Q000254:growth & development / Q000378:metabolism*
chemical_list: D007874:Leghemoglobin; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D005978:Glutathione; D001205:Ascorbic Acid