title: Preliminary toxicity study of dichloromethane extract of Kielmeyera coriacea stems in mice and rats.
abstract: Kielmeyera coriacea Mart. (Clusiaceae), known as "Pau Santo" or "Saco de Boi" in the central Brazilian plateau region, is used to treat several tropical diseases. The present study evaluated the toxic effects of dichloromethane (DcM) extract of Kielmeyera coriacea stems, administered to rodents. In the acute toxicity tests, mice receiving doses of this extract by the oral and intraperitoneal routes, showed reversible effects, with LD50 values of 1503.0 and 538.8 mg/kg, respectively. In the repeated-dose oral (90 days) toxicity tests, male and female Wistar rats were treated by gavage with different doses of DcM extract (5, 25 or 125 mg/kg). In biochemical and haematological evaluations, the results varied widely in respect to dose and sex, with no linear profile, and did not show clinical correlations. In the histopathological examinations, the groups exhibited some changes, but there were no significant differences between the groups compared to the controls. In conclusion, these investigations appeared to indicate the safety of acute and repeated oral administration of the DcM extract of Kielmeyera coriacea stems, which can therefore be continuously used with safety.
authors.affiliation: Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá, Maringá, Paraná, Brazil. simoniobici@yahoo.com.br
authors.forename: Simoni
authors.initials: S
authors.lastname: Obici
authors.forename: Fernanda Jacques
authors.initials: FJ
authors.lastname: Otobone
authors.forename: Vânia Ramos
authors.initials: VR
authors.lastname: da Silva Sela
authors.forename: Kelly
authors.initials: K
authors.lastname: Ishida
authors.forename: José Carlos
authors.initials: JC
authors.lastname: da Silva
authors.forename: Celso Vataru
authors.initials: CV
authors.lastname: Nakamura
authors.forename: Diógenes Aparício
authors.initials: DA
authors.lastname: Garcia Cortez
authors.forename: Elisabeth Aparecida
authors.initials: EA
authors.lastname: Audi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001938:Brazil; D028084:Clusiaceae / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D005260:Female; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D008297:Male; D008519:Medicine, Traditional; D008752:Methylene Chloride / Q000737:chemistry; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage / Q000633:toxicity*; D018547:Plant Stems; D051381:Rats; D017208:Rats, Wistar; D012737:Sex Factors; D023382:Toxicity Tests, Acute
chemical_list: D010936:Plant Extracts; D008752:Methylene Chloride