title: PYP-1, inorganic pyrophosphatase, is required for larval development and intestinal function in C. elegans.
abstract: Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate (PPi) into phosphate (Pi), which provides a thermodynamic driving force for important biosynthetic reactions. The nematode Caenorhabditis elegans gene C47E12.4 encodes a PPase (PYP-1) which shows 54% amino acid identity with human PPase. PYP-1 exhibits specific enzyme activity and is mainly expressed in the intestinal and nervous system. A null mutant of pyp-1 reveals a developmental arrest at early larval stages and exhibits gross defects in intestinal morphology and function. The larval arrest phenotype was successfully rescued by reintroduction of the pyp-1 gene, suggesting that PYP-1 is required for larval development and intestinal function in C. elegans.
authors.affiliation: Cell Dynamics Research Center, Department of Life Science, GIST, Gwangju 500-712, Republic of Korea.
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Ko
authors.forename: Wonhae
authors.initials: W
authors.lastname: Lee
authors.forename: Jae-Ran
authors.initials: JR
authors.lastname: Yu
authors.forename: Joohong
authors.initials: J
authors.lastname: Ahnn
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D017124:Conserved Sequence; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D043564:Inorganic Pyrophosphatase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007422:Intestines / Q000201:enzymology* / Q000254:growth & development*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D016415:Sequence Alignment
chemical_list: D043564:Inorganic Pyrophosphatase