Association of alpha-adducin Gly460Trp polymorphism with coronary artery disease in a Korean population.
OBJECTIVE
Coronary artery disease is caused by multiple genetic and environmental factors. The disease is also closely associated with cardiovascular conditions such as hypertension. In order to investigate any possible role of hypertension candidate genes in the disease development and progression, we examined the association of the polymorphisms of 31 hypertension candidate genes with coronary artery disease.


METHODS
Genetic polymorphisms of 31 hypertension candidate genes were initially screened by resequencing DNA samples from 24 unrelated individuals in a Korean population. Association analysis was performed using 1284 unrelated Korean men, including 749 coronary artery disease subjects and 535 normal healthy controls.


RESULTS
We identified a total of 409 single nucleotide polymorphisms including 40 nonsynonymous single nucleotide polymorphisms, 32 insertions/deletions and four microsatellites. Among 40 nonsynonymous single nucleotide polymorphisms, 29 were examined for an association with coronary artery disease. A significant association with coronary artery disease was observed in a polymorphism of the ADD1 gene (Gly460Trp; +29017G/T) (odds ratio 0.71-0.81; P = 0.01-0.04). The same polymorphism was also associated with the number of arteries with significant coronary artery stenosis in the coronary artery disease patients (P = 0.01) as well as the increase in systolic blood pressure (P = 0.02).


CONCLUSIONS
The ADD1 Gly460Trp polymorphism is significantly associated with an increased risk of coronary artery disease as well as blood pressure, indicating that ADD1 plays a role in the pathogenesis of coronary artery disease as well as hypertension.
Cha
Seung-Hun
SH
National Genome Research Institute, National Institute of Health, Seoul, Seoul, Korea.
Kim
Hung-Tae
HT
Jang
Yangsoo
Y
Park
Sungha
S
Kim
Jae-Jung
JJ
Song
Min Young
MY
Park
Jin-Hyoung
JH
Ryu
Ha-Jung
HJ
Park
Hyun-Young
HY
Yoon
Sung-Joo Kim
SJ
Kimm
Kuchan
K
Lee
Jong-Keuk
JK
Oh
Bermseok
B
D000328:Adult; D019943:Amino Acid Substitution; D002148:Calmodulin-Binding Proteins / Q000235:genetics*; D016022:Case-Control Studies; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics; D003324:Coronary Artery Disease / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000209:etiology / Q000235:genetics; D007723:Korea; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D000099291:Adducins
D002148:Calmodulin-Binding Proteins; D000099291:Adducins