Production of recombinant HIV-1/HBV virus-like particles in Nicotiana tabacum and Arabidopsis thaliana plants for a bivalent plant-based vaccine.
Human immunodeficiency virus (HIV-1) and hepatitis B virus (HBV) spread via similar transmission pathways, and infection by HBV occurs in up to 32% of HIV-1 cases. Here, we describe the successful expression of novel recombinant HIV-1/HBV virus-like particles (VLPs) in Nicotiana tabacum and Arabidopsis thaliana. The production levels and quality of the recombinant VLPs were comparable in the two plants, showing that parameters intrinsic to the recombinant proteins determined their assembly into VLPs. These heterologous VLPs can be used in a bivalent anti-HIV-1/-HBV vaccine, administrated via ingestion of transgenic plants.
Greco
Raffaella
R
Department of Biology, "Cascina Rosa" Botanical Garden, University of Milan, via Celoria 26, 20133 Milan, Italy.
Michel
Marie
M
Guetard
Denise
D
Cervantes-Gonzalez
Minerva
M
Pelucchi
Nilla
N
Wain-Hobson
Simon
S
Sala
Francesco
F
Sala
Monica
M
D016915:AIDS Vaccines / Q000276:immunology; D017360:Arabidopsis / Q000235:genetics / Q000821:virology*; D045744:Cell Line, Tumor; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D017325:Hepatitis B Vaccines / Q000276:immunology; D006515:Hepatitis B virus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D054298:Human Immunodeficiency Virus Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D006801:Humans; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014026:Nicotiana / Q000235:genetics / Q000821:virology*; D019076:Transgenes; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
D016915:AIDS Vaccines; D017325:Hepatitis B Vaccines; D054298:Human Immunodeficiency Virus Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins