Extracts of dietary plants are efficient modulators of nuclear factor kappa B.
Convincing evidence suggest that a plant-based diet is associated with a reduced risk of several chronic diseases, but the mechanisms for this association is not fully elucidated. The transcription factor nuclear factor kappa B (NF-kappa B) plays a critical role in cellular stress-, immune- and inflammatory responses. Also, NF-kappa B is identified as a promising therapeutic target both in cancer and chronic inflammation. We used monocytes stably transfected with a NF-kappa B-luciferase reporter construct in a screening of plant extracts for NF-kappa B modulators. Our aim was to identify dietary components which could induce basal NF-kappa B activity to produce a preconditioning effect, or inhibit induction of disease related NF-kappa B activity. When screening 34 dietary plants for their ability to induce basal NF-kappa B activity or inhibit lipopolysaccharide induced NF-kappa B activity we observed that 23 dietary plant extracts induced basal NF-kappa B activity, while 15 extracts attenuate induced NF-kappa B activation. These results indicate that dietary plants contain compounds that efficiently modulate NF-kappa B activity. We suggest dietary modulation of NF-kappa B may contribute to the observed beneficial effects of dietary plants on the risk of chronic diseases.
Paur
I
I
Department of Nutrition, Faculty of Medicine, University of Oslo, PO Box 1046, 0316 Oslo, Norway.
Austenaa
L M
LM
Blomhoff
R
R
D002460:Cell Line; D004247:DNA / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008156:Luciferases / Q000235:genetics; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D016328:NF-kappa B / Q000187:drug effects*; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000494:pharmacology; D010945:Plants, Edible / Q000737:chemistry*; D012015:Reference Standards; D051996:Transcription Factor RelA / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection; D020298:U937 Cells
D008070:Lipopolysaccharides; D016328:NF-kappa B; D010936:Plant Extracts; D051996:Transcription Factor RelA; D004247:DNA; D008156:Luciferases