Differential Flo8p-dependent regulation of FLO1 and FLO11 for cell-cell and cell-substrate adherence of S. cerevisiae S288c.
Cell-cell and cell-surface adherence represents initial steps in forming multicellular aggregates or in establishing cell-surface interactions. The commonly used Saccharomyces cerevisiae laboratory strain S288c carries a flo8 mutation, and is only able to express the flocculin-encoding genes FLO1 and FLO11, when FLO8 is restored. We show here that the two flocculin genes exhibit differences in regulation to execute distinct functions under various environmental conditions. In contrast to the laboratory strain Sigma1278b, haploids of the S288c genetic background require FLO1 for cell-cell and cell-substrate adhesion, whereas FLO11 is required for pseudohyphae formation of diploids. In contrast to FLO11, FLO1 repression requires the Sin4p mediator tail component, but is independent of the repressor Sfl1p. FLO1 regulation also differs from FLO11, because it requires neither the KSS1 MAP kinase cascade nor the pathways which lead to the transcription factors Gcn4p or Msn1p. The protein kinase A pathway and the transcription factors Flo8p and Mss11p are the major regulators for FLO1 expression. Therefore, S. cerevisiae is prepared to simultaneously express two genes of its otherwise silenced FLO reservoir resulting in an appropriate cellular surface for different environments.
Fichtner
Lars
L
Institut für Mikrobiologie und Genetik, DFG Research Center for Molecular Physiology of the Brain (CMPB), Georg-August Universität Göttingen, Grisebachstr. 8, D-37077 Göttingen, Germany.
Schulze
Florian
F
Braus
Gerhard H
GH
D002448:Cell Adhesion; D015966:Gene Expression Regulation, Fungal; D037241:Mannose-Binding Lectins; D056892:Mediator Complex; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000096:biosynthesis*; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology*; D015534:Trans-Activators / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology
C087578:FLO1 protein, S cerevisiae; C110690:FLO11 protein, S cerevisiae; C100940:FLO8 protein, S cerevisaie; C109358:MSS11 protein, S cerevisiae; D037241:Mannose-Binding Lectins; D056892:Mediator Complex; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; C063404:SFL1 protein, S cerevisiae; C076878:SIN4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C075580:FUS3 protein, S cerevisiae; C541984:KSS1 protein, S cerevisiae; D020928:Mitogen-Activated Protein Kinases