Primary molecular features of self-incompatible and self-compatible F(1) seedling from apricot (Prunus armeniaca L.) Katy x Xinshiji.
Expression of the S-RNase genes in the self-compatible (SC) apricot (Prunus armeniaca L.) cultivar Katy, the self-incompatible (SI) cultivar Xinshiji and their F(1) seedling was examined in this study. Three S-genotypes, S(9)Sc (Sc, self-compatibility S-gene absent from the style), S(8)S(9), and S(8)S(10), were obtained. Seedlings with S-RNase that migrated as a single band in gel electrophoresis were SC, despite high transcript abundance, and those with S-RNase that migrated as two bands were SI with high transcript abundance or SC with low transcript expression. S(8)-RNase was induced in SI cultivars only 24 h after self-pollination, indicating post-transcriptional regulation of S(8)-RNase in SI apricots. A Proteomic study showed that 35 protein spots were synthesized differently between SC and SI pistils. Fifteen of the 35 protein spots were identified; nine proteins, including receptor protein kinase-like protein, reversibly glycosylated polypeptide-2, and isoflavone reductase-like protein, were detected only in the SC pistils; while nine proteins, including actin 7, a putative serine/threonine kinase, and S-RNase, were detected only in the SI pistils. A mitochondrial NAD-dependent malate dehydrogenase and a probable elongation factor G were up-regulated, while heat shock cognate 70 was down-regulated in the SC pistils compared to those in the SI pistils. The results suggest that the proteins responsible for self-compatibility and self-incompatibility may be different.
Feng
J R
JR
Horticultural Department, Shihezi University, Shihezi, Xinjiang 832003, China. fengjr102@126.com
Chen
X S
XS
Yuan
Z H
ZH
Zhang
L J
LJ
Ci
Z J
ZJ
Liu
X L
XL
Zhang
C Y
CY
D004586:Electrophoresis; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D010940:Plant Proteins / Q000032:analysis*; D040901:Proteomics; D027861:Prunus; D012260:Ribonucleases / Q000235:genetics*; D036226:Seedlings / Q000201:enzymology*
D010940:Plant Proteins; D012260:Ribonucleases; C022542:ribonuclease S