[Preliminary analyses for influence of mutant PrPs with different number of octapeptide].
OBJECTIVE
The present study was conducted to understand the effects of PrP in different octapeptide repeats on proliferation of HeLa cells.


RESULTS
Mutant PrPs with octapeptide repeat insertion were transiently expressed in HeLa cells and their results of MTT assay showed stronger cytotoxic effect on the proliferation of cells than wild-type PrP. Annexin V/PI assay also demonstrated that the expression of mutant PrPs was much easier to induce apoptosis than wild-type in HeLa cells. The percentage of both early and late stage apoptosis in mutant groups were significantly higher than that of wild type.


CONCLUSIONS
These data suggest that the expression of mutant PrPs associated with familial CJD is much easier to induce apoptosis in cultured cells than expression of wild type PrP.
Han
Lu
L
Department of Prion Disease, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Wan
Yan-Zhen
YZ
Han
Jun
J
Chen
Lan
L
Sun
Li
L
Wang
Xiao-Fan
XF
Huang
Yin-Xia
YX
Dong
Chen-Fang
CF
Jiang
Hui-Ying
HY
Dong
Xiao-Ping
XP
D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D015153:Blotting, Western; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics / Q000502:physiology; D003124:Colorimetry; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D009842:Oligopeptides / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D000072002:Prion Proteins; D011328:Prions / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D014162:Transfection
D009842:Oligopeptides; C512656:PRNP protein, human; D000072002:Prion Proteins; D011328:Prions