Evidence for the presence of stem cell-like progenitor cells in human adult pancreas.
The origin of cells replacing ageing beta-cells in adult life is unknown. This study assessed the expression of classic stem cell markers: Oct4, Sox2 and CD34 in islet-enriched fractions versus exocrine cell-enriched fractions from 25 adult human pancreases following human islet isolation. Expression of Oct4, Sox2 and CD34 mRNAs was found in all cell samples, with no significant differences between endocrine and exocrine cell fractions. Immunohistochemical staining for Oct4, Sox2, CD133, CD34, CK19, insulin and nestin on human pancreas sections showed that the majority of Oct4(+ve) cells were found in the walls of small ducts. Similar localisations were observed for Sox2(+ve) cells. The majority of Sox2(+ve) cells were found to co-express Oct4 proteins, but not vice versa. Cells positive for Oct4 and Sox2 appeared to be a unique cell population in the adult human pancreases without co-expression for CK19, CD34, CD133, insulin and nestin proteins. The numbers of Oct4(+ve) and Sox2(+ve) cells varied among donors and were approximately 1-200 and 1-30 per 100 000 pancreatic cells respectively.
Zhao
Min
M
Department of Diabetes, Endocrinology and Internal Medicine, King's College London School of Medicine, Bessemer Road, London SE5 9PJ, UK.
Amiel
Stephanie A
SA
Christie
Michael R
MR
Muiesan
Paolo
P
Srinivasan
Parthi
P
Littlejohn
Wendy
W
Rela
Mohamed
M
Arno
Matthew
M
Heaton
Nigel
N
Huang
Guo Cai
GC
D000071916:AC133 Antigen; D000328:Adult; D000375:Aging / Q000378:metabolism; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis / Q000235:genetics; D015415:Biomarkers / Q000032:analysis; D005260:Female; D006023:Glycoproteins / Q000032:analysis; D024002:HMGB Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D050814:Octamer Transcription Factor-3 / Q000032:analysis / Q000235:genetics; D010179:Pancreas / Q000737:chemistry / Q000166:cytology*; D010183:Pancreatic Ducts / Q000737:chemistry / Q000166:cytology; D010455:Peptides / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055748:SOXB1 Transcription Factors; D013194:Staining and Labeling; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology*; D014019:Tissue Donors; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D015415:Biomarkers; D006023:Glycoproteins; D024002:HMGB Proteins; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; C000606023:PROM1 protein, human; D010455:Peptides; D012333:RNA, Messenger; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D014157:Transcription Factors