title: Structural characterizations of lipids A by MS/MS of doubly charged ions on a hybrid linear ion trap/orbitrap mass spectrometer.
abstract: Here, a new 'one pot' and fast approach is described, based on electrospray ionization (ESI) of negative ions by using a hybrid linear ion trap/orbitrap mass spectrometer (LTQ/orbitrap) for MS and MS/MS analysis. By this method the distribution of the primary and secondary acyl residues of the intact lipid A is inferred by analysis of the ESI spectra measured in positive and negative mode. The analysis of these data allows an unequivocal assignment of the fatty acid distribution. This methodology was successfully tested on two different lipid A with known structures, deriving from the Agrobacterium tumefaciens and Escherichia coli lipopolysaccharides (LPS).
authors.affiliation: Dipartimento di Chimica Organica e Biochimica, Universitá di Napoli Federico II, via Cynthia 4, 80126 Napoli, Italy.
authors.forename: Alba
authors.initials: A
authors.lastname: Silipo
authors.forename: Cristina
authors.initials: C
authors.lastname: De Castro
authors.forename: Rosa
authors.initials: R
authors.lastname: Lanzetta
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Parrilli
authors.forename: Giorgio
authors.initials: G
authors.lastname: Vago
authors.forename: Luisa
authors.initials: L
authors.lastname: Sturiale
authors.forename: Angela
authors.initials: A
authors.lastname: Messina
authors.forename: Domenico
authors.initials: D
authors.lastname: Garozzo
mesh_terms: D016960:Agrobacterium tumefaciens / Q000737:chemistry*; D000838:Anions / Q000032:analysis; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000032:analysis*; D008070:Lipopolysaccharides / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D000838:Anions; D029968:Escherichia coli Proteins; D008070:Lipopolysaccharides