[Effect of cytomegalovirus infection on expression of matrix metalloproteinase-2 in human endothelial cells].
OBJECTIVE
To investigate the effect of cytomegalovirus infection on expression of matrix metalloproteinase-2 in human endothelial cells.


METHODS
Human umbilical vein endothelial cells were cultured in vitro. Cells between 3-6 passages were infected with cytomegalovirus for different time. Expression of matrix metalloproteinase-2 mRNA was analyzed by reverse transcription-polymerase chain reaction, matrix metalloproteinase-2 activity was detected by gel zymography.


RESULTS
Expression of matrix metalloproteinase-2 mRNA and its activity 6 hours after infection was almost equal to control, and was greatly enhanced 12 and 24 hours after infection.


CONCLUSIONS
Cytomegalovirus infection up-regulates expression of matrix metalloproteinase-2 in human endothelial cells. It might be one of the mechanisms that cytomegalovirus is involved in atherosclerosis.
Wang
Rui-Jin
RJ
Department of Neurology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, China.
Tuo
Hou-Zhen
HZ
Wang
De-Xin
DX
Wang
Jia-Wei
JW
Feng
Zi-Jing
ZJ
D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000502:physiology*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D015971:Gene Expression Regulation, Enzymologic; D054884:Host-Pathogen Interactions; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014471:Umbilical Veins / Q000166:cytology
D012333:RNA, Messenger; D020778:Matrix Metalloproteinase 2