The Arabidopsis RNA-binding protein FCA requires a lysine-specific demethylase 1 homolog to downregulate FLC.
A repressor of the transition to flowering in Arabidopsis is the MADS box protein FLOWERING LOCUS C (FLC). FCA, an RNA-binding protein, and FY, a homolog of the yeast RNA 3' processing factor Pfs2p, downregulate FLC expression and therefore promote flowering. FCA/FY physically interact and alter polyadenylation/3' processing to negatively autoregulate FCA. Here, we show that FCA requires FLOWERING LOCUS D (FLD), a homolog of the human lysine-specific demethylase 1 (LSD1) for FLC downregulation. FCA also partially depends on DICER-LIKE 3, involved in chromatin silencing. fca mutations increased levels of unspliced sense FLC transcript, altered processing of antisense FLC transcripts, and increased H3K4 dimethylation in the central region of FLC. These data support a close association of FCA and FLD in mediating H3K4 demethylation and thus transcriptional silencing of FLC and reveal roles for antisense RNA processing and DCL3 function in this regulation.
Liu
Fuquan
F
Department of Cell and Developmental Biology, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
Quesada
Victor
V
Crevillén
Pedro
P
Bäurle
Isabel
I
Swiezewski
Szymon
S
Dean
Caroline
C
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D047369:Chromatin Immunoprecipitation; D015536:Down-Regulation; D020868:Gene Silencing; D006655:Histone Deacetylases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D006657:Histones / Q000378:metabolism; D026161:MADS Domain Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D008745:Methylation; D008969:Molecular Sequence Data; D009154:Mutation; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D043244:Ribonuclease III / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic
D029681:Arabidopsis Proteins; C418902:At5g10140 protein, Arabidopsis; C106535:Flowering time control protein FCA, Arabidopsis; D006657:Histones; D026161:MADS Domain Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C519654:DCL3 protein, Arabidopsis; D043244:Ribonuclease III; C479923:flowering locus D protein, Arabidopsis; D006655:Histone Deacetylases