title: [Detection of invasive aspergillosis by PCR and real-time PCR--benefits and drawbacks].
abstract: PCR detection of fungal pathogens in clinical samples has been discussed in journals for more than two decades. However, its use for diagnosing invasive aspergillosis is still controversial, despite the fact that molecular methods are routinely used in various fields of modern microbiology. These are e. g. genotyping of bacterial strains resistant to antibiotics, molecular epidemiology or routine detection of viral infections in clinical material. PCR methods have made the diagnostic applications faster, simpler and more accurate. This review deals with issues related to molecular methods for diagnosing invasive fungal infections and the main factors limiting their use in everyday clinical practice.
authors.affiliation: Laboratory for Molecular and Cell Biology, Department of Internal Medicine and Hematooncology, Masaryk University School of Medicine, Brno, Czech Republic. mlengerova@fnbrno.cz
authors.forename: Martina
authors.initials: M
authors.lastname: Lengerová
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Rácil
authors.forename: Kristýna
authors.initials: K
authors.lastname: Hrncírová
authors.forename: Pavlína
authors.initials: P
authors.lastname: Volfová
authors.forename: Jana
authors.initials: J
authors.lastname: Lochmanová
authors.forename: Dana
authors.initials: D
authors.lastname: Dvoráková
authors.forename: Jirí
authors.initials: J
authors.lastname: Mayer
mesh_terms: D001228:Aspergillosis / Q000175:diagnosis*; D001230:Aspergillus / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000032:analysis*; D006801:Humans; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D004271:DNA, Fungal