Nisin-triggered activity of Lys44, the secreted endolysin from Oenococcus oeni phage fOg44.
The intrinsic resistance of Oenococcus oeni cells to the secreted endolysin from oenophage fOg44 (Lys44) was investigated. Experiments with several antimicrobials support the hypothesis that the full activity of Lys44 requires sudden ion-nonspecific dissipation of the proton motive force, an event undertaken by the fOg44 holin in the phage infection context.
Nascimento
João Gil
JG
Instituto de Ciência Aplicada e Tecnologia da Faculdade de Ciências da Universidade de Lisboa, 1749-016 Lisboa, Portugal.
Guerreiro-Pereira
Maria Carolina
MC
Costa
Sérgio Fernandes
SF
São-José
Carlos
C
Santos
Mário Almeida
MA
D001435:Bacteriophages / Q000472:pathogenicity / Q000502:physiology*; D002473:Cell Wall / Q000502:physiology / Q000821:virology; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D007113:Immunity, Innate; D007777:Lactobacillaceae / Q000502:physiology / Q000821:virology*; D009561:Nisin / Q000378:metabolism*; D014764:Viral Proteins / Q000502:physiology*
D004279:DNA, Viral; D014764:Viral Proteins; D009561:Nisin; D004247:DNA