title: Multipotency and growth characteristic of periosteum-derived progenitor cells for chondrogenic, osteogenic, and adipogenic differentiation.
abstract: The mesengenic multipotency of cryopreserved periosteum-derived progenitor cells (PDPCs) for chondrogenesis, osteogenesis and adipogenesis was investigated. Differentiation was verified using RT-PCR and histological analysis. For characterization, FACS analysis was performed with specific surface markers of mesenchymal stem cells (MSCs). Among PDPCs, unsorted periosteum-derived cells (PDCs) and dermal fibroblasts, the most distinct characteristics were found to be CD9, CD105, and CD166. In addition, these markers in PDPCs were continuously maintained until passage 15. We developed a rapid method for the isolation of PDPCs that can differentiate into mesodermal lineages and provide enough cells in a short period of time for allogeneic cell therapy.
authors.affiliation: Department of Biological Engineering, Inha University, Incheon 402-751, Korea.
authors.forename: Yong-Soo
authors.initials: YS
authors.lastname: Choi
authors.forename: Sang-Eun
authors.initials: SE
authors.lastname: Noh
authors.forename: Sang-Min
authors.initials: SM
authors.lastname: Lim
authors.forename: Chang-Woo
authors.initials: CW
authors.lastname: Lee
authors.forename: Chul-Soo
authors.initials: CS
authors.lastname: Kim
authors.forename: Moon-Whan
authors.initials: MW
authors.lastname: Im
authors.forename: Moon-Hee
authors.initials: MH
authors.lastname: Lee
authors.forename: Dong-Il
authors.initials: DI
authors.lastname: Kim
mesh_terms: D050156:Adipogenesis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D015703:Antigens, CD / Q000032:analysis; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D000071063:Endoglin; D005326:Fetal Proteins / Q000032:analysis; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000032:analysis; D039902:Multipotent Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D010521:Periosteum / Q000166:cytology*; D011956:Receptors, Cell Surface / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D060245:Tetraspanin 29; D020410:Activated-Leukocyte Cell Adhesion Molecule
chemical_list: D015703:Antigens, CD; D015816:Cell Adhesion Molecules, Neuronal; D000071063:Endoglin; D005326:Fetal Proteins; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D060245:Tetraspanin 29; C105613:ALCAM protein, human; D020410:Activated-Leukocyte Cell Adhesion Molecule; C554869:CD9 protein, human; C064206:ENG protein, human