PEGylated lipoplexes: preparation protocols affecting DNA condensation and cell transfection efficiency.
The inclusion of poly(ethylene glycol) monolaurate in liposomes formulated with dimyristoyl-sn-glycero-3-phosphocholine and certain cationic gemini surfactants improves their capability of condensing DNA into a psi phase and transfecting it into cells. Both the condensation, observed by circular dichroism, and the transfection efficiency are strongly effected by the protocol of inclusion of the polymer in the formulations. The highest transfection efficiency is observed in correspondence of the highest extent of DNA condensation.
Bombelli
Cecilia
C
Istituto di Metodologie Chimiche del CNR and Dipartimento di Chimica, Università degli Studi di Roma La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy.
Faggioli
Francesca
F
Luciani
Paola
P
Mancini
Giovanna
G
Sacco
Maria Grazia
MG
D000818:Animals; D019556:COS Cells; D002412:Cations; D002522:Chlorocebus aethiops; D002942:Circular Dichroism; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008081:Liposomes; D011092:Polyethylene Glycols; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D014162:Transfection
D002412:Cations; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; C526823:poly(ethylene glycol) monolaurate; D049452:Green Fluorescent Proteins; D011092:Polyethylene Glycols; D004247:DNA; D004134:Dimyristoylphosphatidylcholine