title: Acquisition of different carbapenem resistance mechanisms by an epidemic clonal lineage of Pseudomonas aeruginosa.
abstract: Multidrug-resistant isolates of a clonal lineage of Pseudomonas aeruginosa producing the VIM-2 metallo-beta-lactamase (MBL), involved in a large outbreak in an Italian hospital, were compared with MBL-negative strains that had caused outbreaks in two French hospitals. Although the isolates had different carbapenem MICs, the VIM-2-producing isolates from Italy carried identical, or very similar, allelic forms of the oprD gene, harboured a common class 1 integron, belonged to the same multilocus sequence type (ST111), and showed macrorestriction profiles that were related to those of the MBL-negative French strains. These results support the concept of independent acquisition of resistance determinants by members of a widespread clonal lineage of P. aeruginosa.
authors.affiliation: Dipartimento di Scienze Biomediche, Sezione di Microbiologia, Università di Trieste, Trieste, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Edalucci
authors.forename: R
authors.initials: R
authors.lastname: Spinelli
authors.forename: L
authors.initials: L
authors.lastname: Dolzani
authors.forename: M Letizia
authors.initials: ML
authors.lastname: Riccio
authors.forename: V
authors.initials: V
authors.lastname: Dubois
authors.forename: E Angelo
authors.initials: EA
authors.lastname: Tonin
authors.forename: G M
authors.initials: GM
authors.lastname: Rossolini
authors.forename: C
authors.initials: C
authors.lastname: Lagatolla
mesh_terms: D015780:Carbapenems / Q000494:pharmacology*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005602:France / Q000453:epidemiology; D006801:Humans; D032023:Integrons; D007558:Italy / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D018272:Porins / Q000235:genetics; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000032:analysis
chemical_list: D015780:Carbapenems; D004269:DNA, Bacterial; D018272:Porins; C077157:OprD protein, Pseudomonas aeruginosa; C425108:beta-lactamase bla(vim-2); D001618:beta-Lactamases