Healing properties of malabaricone B and malabaricone C, against indomethacin-induced gastric ulceration and mechanism of action.
The healing activity of malabaricone B and malabaricone C, the major antioxidant constituents of the spice Myristica malabarica against the indomethacin-induced gastric ulceration in mice has been studied. The histological indices revealed maximum ulceration on the 3rd day after indomethacin administration, which was effectively healed by malabaricone B, malabaricone C (each 10 mg/kg body weight/day) and omeprazole (3 mg/kg body weight/day) for 3 days. Compared to the untreated ulcerated mice, treatment with malabaricone B, malabaricone C and omeprazole reduced the ulcer indices by 60.3% (P<0.01), 88.4% and 86.1% respectively (P<0.001). All the test samples accelerated ulcer healing than observed in natural recovery even after 7 days. Stomach ulceration reduced the total antioxidant status of plasma by 41% (P<0.05), which was significantly increased by malabaricone B (36%, P<0.01), malabaricone C (61%, P<0.001) and omeprazole (53%, P<0.001). Compared to the ulcerated untreated mice, those treated with malabaricone B reduced the levels of thiobarbituric acid reactive substances and protein carbonyls by 17% and approximately 34% respectively (P<0.05), while malabaricone C and omeprazole reduced the parameters almost equally (approximately 30%, P<0.01, and approximately 40%, P<0.01 respectively). Likewise, all the test samples reduced the oxidation of protein and non-protein thiols significantly (P<0.05). The antioxidant activity of the test samples could partly account their healing capacities. However, the differential potency of them was explainable by considering their relative abilities to modulate mucin secretion, PGE(2) synthesis and expression of EGF receptor and COX isoforms, malabaricone C being most effective in controlling all these factors.
Banerjee
Debashish
D
Department of Biochemistry, Dr. B.C. Roy Post Graduate Institute of Basic Medical Sciences & IPGME&R, 244B, Acharya Jagadish Chandra Bose Road, Kolkata - 700 020, India.
Bauri
Ajay K
AK
Guha
Ranjit K
RK
Bandyopadhyay
Sandip K
SK
Chattopadhyay
Subrata
S
D000818:Animals; D000897:Anti-Ulcer Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity; D051545:Cyclooxygenase 1 / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D066246:ErbB Receptors / Q000378:metabolism; D005638:Fruit; D005752:Gastric Mucins / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D007213:Indomethacin; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D026324:Myristicaceae / Q000737:chemistry; D009853:Omeprazole / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D010503:Periodic Acid-Schiff Reaction; D010936:Plant Extracts / Q000494:pharmacology; D050050:Protein Carbonylation / Q000187:drug effects; D012118:Resorcinols / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity; D013270:Stomach / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
D000897:Anti-Ulcer Agents; D000975:Antioxidants; D005752:Gastric Mucins; D008565:Membrane Proteins; D010936:Plant Extracts; D012118:Resorcinols; C071788:malabaricone B; C071787:malabaricone C; D006003:Glycogen; C077623:Ptgs2 protein, mouse; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496512:Ptgs1 protein, mouse; D066246:ErbB Receptors; D015232:Dinoprostone; D009853:Omeprazole; D007213:Indomethacin