Alterations of pulmonary zinc homeostasis and cytokine production following traumatic brain injury in rats.
Previous studies have shown that labile zinc and inflammatory mediators participate in many pathophysiological processes. The present study investigated the effects of traumatic brain injury (TBI) on the levels of labile zinc and certain proinflammatory factors in rat lung. Male Wistar rats were randomly assigned to 7 groups as follows: normal group, group with sham operation, and TBI groups that were sacrificed respectively at 1, 6, 24, and 72 hr, and on day 7 post-injury. Pulmonary labile zinc, tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-8, and wet/dry weight ratio were measured at the specified time intervals. TBI caused a gradual increase of pulmonary labile zinc as demonstrated by fluorescence staining with Zinpyr-4 (ZP4). The levels of TNF-alpha and IL-8 and the lung wet/dry weight ratios were higher in the TBI groups compared to the normal and sham-operated groups (p <0.05). There were highly positive correlations between the intensity of ZP4 fluorescence and the pulmonary levels of TNF-alpha and IL-8. The results suggest that TBI induces rapid increases of labile zinc and inflammatory mediators in lung, which may participate in the pathogenesis of acute lung injury.
Zhu
Lin
L
Department of Neurosurgery, Jinling Hospital, 305 East Zhongshan Road, Nanjing, 210002, P.R. China.
Yan
Wei
W
Qi
Meng
M
Hu
Ze Lan
ZL
Lu
Ting Jia
TJ
Chen
Min
M
Zhou
Jin
J
Hang
Chun Hua
CH
Shi
Ji Xin
JX
D000818:Animals; D001930:Brain Injuries / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D018450:Disease Progression; D005456:Fluorescent Dyes / Q000378:metabolism; D006706:Homeostasis; D016209:Interleukin-8 / Q000378:metabolism; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation; D015032:Zinc / Q000378:metabolism*
D016207:Cytokines; D005456:Fluorescent Dyes; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D015032:Zinc