[Protective effect of Huaxia shallot preparation on human umbilical vein endothelial cell injury induced by oxidized low density lipoprotein and its mechanism].
OBJECTIVE
To observe the protective effect of Huaxia shallot preparation on human umbilical vein endothelial cell (HUVEC) injury induced by oxidized low density lipoprotein (Ox-LDL) in vitro.


METHODS
Ox-LDL was prepared and identified, and HUVECs were cultured. After 2-hour intervention of different drugs and 24-hour following intervention of Ox-LDL, the number of HUVECs was observed by phase contrast optical microscope and the activity of the HUVECs was observed by methyl thiazolyl tetrazolium (MTT) technique. Superoxide dismutase (SOD) activity and nitric oxide (NO) content were assayed by respective kit. The protein expressions and mRNA levels of peroxisome proliferators activated receptor gamma(PPAR-gamma) and endothelial nitric oxide synthase (eNOS) were measured by western blot technique and reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
Ox-LDL could increase the apoptosis rate of the HUVECs and decrease the NO release as compared with the blank control group (P<0.05). These effects induced by Ox-LDL were all significantly inhibited by Huaxia shallot preparation. It could up-regulate the protein expressions and mRNA levels of PPAR-gamma and eNOS significantly (P<0.05). Huaxia shallot preparation could decrease the apoptosis rate of the HUVECs.


CONCLUSIONS
Ox-LDL may be involved in the initiation and progression of atherosclerosis by injuring the endothelial cells directly and may cause the endothelial dysfunction. Huaxia shallot preparation can protect against Ox-LDL induced endothelial cell injury by up-regulating the protein expressions and mRNA levels of PPAR-gamma and eNOS. It suggests that Huaxia shallot preparation may play a role in the prevention and treatment of cardiovascular disease.
Zhang
Jie-mei
JM
Unit of Cardiology, Institute of Integrative Medicine, Wuhan First Hospital, and Human Genome Research Centre, Life and Technology College, Huazhong University of Science and Technology, Wuhan, Hubei Province 430022, China. Zhangjiemei66@163.com
Guo
Jie
J
Tu
Xin
X
Shi
Zhao-hong
ZH
Hao
Jian-jun
JJ
Ke
Yu-he
YH
Guan
Jiang-feng
JF
He
Juan-juan
JJ
D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects / Q000473:pathology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D027781:Shallots / Q000737:chemistry*; D014471:Umbilical Veins / Q000166:cytology
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D008077:Lipoproteins, LDL; D047495:PPAR gamma; D012333:RNA, Messenger; C099252:oxidized low density lipoprotein; D052250:Nitric Oxide Synthase Type III