Cells tracking in a live zebrafish embryo.
We designed a set of procedures for achieving the tracking of cell nuclei and the identification of cell divisions in live zebrafish embryos using 3D+time images acquired by confocal laser scanning microscopy (CLSM). Our strategy includes image signal enhancement with feature preserving denoising algorithm, automated identification of the nuclei position, extraction of the optical flow from 3D images sequences and tracking of nuclei.
Melani
Camilo
C
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina and Dipartimento di Elettronica, Informatica e Sistemistica, Università di Bologna, Italy. camilo@dc.uba.ar
Campana
Matteo
M
Lombardot
Benoit
B
Rizzi
Barbara
B
Veronesi
Federico
F
Zanella
Cecilia
C
Bourgine
Paul
P
Mikula
Karol
K
Peyriéras
Nadine
N
Sarti
Alessandro
A
D000465:Algorithms; D000818:Animals; D001185:Artificial Intelligence; D004625:Embryo, Nonmammalian / Q000166:cytology*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015027:Zebrafish / Q000033:anatomy & histology* / Q000196:embryology*