Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration.
We made a three-dimensional (3-D) nanofibrous fibroin scaffold (NFS) with high porosity (94%) and examined its feasibility in bone regeneration. Under scanning electron microscopy, MC3T3-E1 preosteoblasts on the scaffold showed more spread on the first day after seeding compared with a 2-D scaffold. MTT assay showed significantly increased proliferation in 3-D NFS compared with 2-D NFS 7 days after seeding (P < 0.05). Western immunoblotting for activated paxillin, FAK, AKT, C-Src, and ERK1/2 antibodies showed signals from the extracellular matrix were significantly increased in 3-D NFS. Newly developed 3-D electrospun NFS may be a good candidate for use in bone regeneration.
Ki
Chang Seok
CS
Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul, Republic of Korea.
Park
Sook Young
SY
Kim
Hyun Jeong
HJ
Jung
Hong-Moon
HM
Woo
Kyung Mi
KM
Lee
Jung Weon
JW
Park
Young Hwan
YH
D001672:Biocompatible Materials; D001861:Bone Regeneration; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D005349:Fibroins / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D010012:Osteogenesis; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D005349:Fibroins