title: Recent developments in the chemistry, analysis and control for the manufacture of therapeutic-grade synthetic oligonucleotides.
abstract: Significant developments in oligonucleotide chemistry, which occurred between 1980 and 2000, led to several oligonucleotide-based therapeutics entering clinical trials. Improvements in synthesis and purification methods, along with analytical techniques have facilitated the large-scale manufacture of high quality oligonucleotides to support these clinical trials.
authors.affiliation: Archemix Corporation, 300 Third Street, Cambridge, MA 02142, USA. Nanda.Sinha@comcast.net
authors.forename: Nanda D
authors.initials: ND
authors.lastname: Sinha
authors.forename: Dennis P
authors.initials: DP
authors.lastname: Michaud
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods / Q000639:trends; D004339:Drug Compounding / Q000379:methods / Q000639:trends; D015195:Drug Design; D006801:Humans; D008956:Models, Chemical; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000627:therapeutic use; D011083:Polycyclic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000627:therapeutic use
chemical_list: D009841:Oligonucleotides; D011083:Polycyclic Compounds