The inhibition of dentifrice containing the lotus leaf-derived inhibitor on periodontitis-related bacteria in vitro.
OBJECTIVE
To search for a substance in lotus leaf that could efficiently be used in toothpaste for preventing periodontal disease.


METHODS
Dried lotus leaves were hydrated and fractionated using 40% butanol and 40% ethanol extraction. The extracts and the residue were evaluated by antimicrobial sensitivity tests of broth micro-dilution methods on 96-microwell plates for inhibition of five species of oral bacteria: Actinobacillus actinomycetemcomitans Y4, Actinomyces viscosus 19246, Porphyromonas gingivalis 33277, Fusobacterium nucleatum 25586, and Actinomyces naeslundii wvl 45. The most active antimicrobial extract was then ready to use in toothpaste. A water extract of the toothpaste was prepared and its inhibitory activity tested. The potency of the lotus leaf extracts was compared to inhibition observed by three commercially available toothpastes.


RESULTS
The butanol-extracted material was separated into four fractions on silica gel. Fraction #3, the fraction containing the most potent inhibitor, was formulated into toothpaste. The toothpaste containing the extract showed an inhibition effect on suspicious peridontitis-causing bacteria.


CONCLUSIONS
An extract from the lotus leaf is effective in inhibiting peridontitis-causing bacteria. The toothpaste containing the lotus leaf-derived inhibitor could be useful in preventing periodontal disease.
Li
Ming-Yu
MY
Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University, China. lmy1691@online.sh.cn
Xu
Zhu-Ting
ZT
D000890:Anti-Infective Agents / Q000627:therapeutic use; D003802:Dentifrices / Q000737:chemistry* / Q000627:therapeutic use; D000070116:Lotus; D010518:Periodontitis / Q000188:drug therapy* / Q000382:microbiology; D008517:Phytotherapy / Q000379:methods*; D018515:Plant Leaves; D012680:Sensitivity and Specificity
D000890:Anti-Infective Agents; D003802:Dentifrices