The anti-invasive effect of lucidenic acids isolated from a new Ganoderma lucidum strain.
Ganoderma lucidum is a well-known mushroom with various pharmacological effects that has been used for health and longevity purposes. The objective of this study was to investigate the anti-invasive effect of lucidenic acids isolated from a new G. lucidum strain (YK-02) against human hepatoma carcinoma (HepG(2)) cells. Triterpenoid components in the ethanol extract of G. lucidum (YK-02) were separated by means of a semi-preparative RP HPLC. Four major peaks were separated and crystallized from triterpenoids fraction, and were identified as lucidenic acids A, B, C, and N according to their spectroscopic values of (1)H NMR and MS. Treatment of the lucidenic acids (50 microM) in the presence of 200 nM phorbol 12-myristate 13-acetate (PMA) after 24 h of incubation all resulted in significant inhibitory effects on PMA-induced MMP-9 activity and invasion of HepG(2 )cells. The results indicate that the lucidenic acids isolated from G. lucidum (YK-02) are anti-invasive bioactive components on hepatoma cells.
Weng
Chia-Jui
CJ
Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan.
Chau
Chi-Fai
CF
Chen
Kuang-Dee
KD
Chen
Deng-Hai
DH
Yen
Gow-Chin
GC
D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D002793:Cholic Acids / Q000008:administration & dosage* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D032902:Ganoderma / Q000737:chemistry*; D006801:Humans; D008113:Liver Neoplasms; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D009361:Neoplasm Invasiveness / Q000517:prevention & control*; D013729:Terpenes / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014315:Triterpenes / Q000008:administration & dosage / Q000302:isolation & purification
D002793:Cholic Acids; D061965:Matrix Metalloproteinase Inhibitors; D013729:Terpenes; D014315:Triterpenes; C481150:lucidenic acid A; C527145:lucidenic acid B; C527146:lucidenic acid C; C437028:lucidenic acid N; D020780:Matrix Metalloproteinase 9; D013755:Tetradecanoylphorbol Acetate