A more efficient enzyme-linked immunosorbent assay for measurement of alpha-synuclein in cerebrospinal fluid.
We describe a modification of a previously described assay for the quantification of alpha-synuclein in naive cerebrospinal fluid, which allows for a more efficient quantification of alpha-synuclein. Detection limit of the assay is 3.8 ng/ml and the assay is linear until 300 ng/ml. Inter-assay and intra-assay coefficients of variation are below 15% in a wide range of concentrations. Mean recovery of the assay is 94%. The 95% upper limit of the reference range (p95) in a group of neurological controls above the age of 45 years is 62 ng/ml. This assay can be routinely applied for quantification of alpha-synuclein in cerebrospinal fluid, but not in serum, and this may serve as a possible biomarker for alpha-synucleinopathies such as Parkinson's disease and multiple system atrophy.
van Geel
Wieneke J A
WJ
Laboratory of Pediatrics and Neurology, Radboud University Nijmegen Medical Centre, The Netherlands.
Abdo
W Farid
WF
Melis
René
R
Williams
Sonja
S
Bloem
Bastiaan R
BR
Verbeek
Marcel M
MM
D000368:Aged; D001927:Brain Diseases / Q000134:cerebrospinal fluid; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012189:Retrospective Studies; D013129:Spinal Puncture / Q000379:methods; D051844:alpha-Synuclein / Q000134:cerebrospinal fluid*
D051844:alpha-Synuclein