[Study on correlation between aquaporin 3, 4 gene expression in gastric mucosa and severity of Pi-Wei damp-heat syndrome in patients with chronic superficial gastritis].
OBJECTIVE
To explore the relationship between aquaporin 3,4 (AQP3, AQP4) gene expression in gastric mucosa and severity of Pi-Wei damp-heat syndrome (PWDHS) in patients with chronic superficial gastritis (CSG).


METHODS
Gastric mucosa taken from the upper part of gastric corpus was collected under gastroscope and preserved in liquid nitrogen. The gene expression of AQP3 and AQP4 was determined quantitatively by fluorescent PCR.


RESULTS
The gene expression of AQP3 and AQP4 in patients with PWDHS of moderate and severe degree was higher than that in those of mild degree and in healthy persons respectively (P <0.05 and P <0.01); and the gene expression of AQP3 in patients with PWDHS of severe degree was higher than that in those of moderate degree (P<0.05).


CONCLUSIONS
The gene expression of AQP3 and AQP4 in gastric mucosa was correlative with the severity of PWDHS in patients with chronic superficial gastritis, the severer the syndrome, the higher the gene expression.
Mei
Wu-xuan
WX
Department of Gastroenterology, Xianning College, Hubei. meifeil@sina.com
Lao
Shao-xian
SX
Zhou
Zheng
Z
D000328:Adult; D051400:Aquaporin 3 / Q000235:genetics*; D051401:Aquaporin 4 / Q000235:genetics*; D002908:Chronic Disease; D003937:Diagnosis, Differential; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism* / Q000473:pathology; D005756:Gastritis / Q000175:diagnosis / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D008297:Male; D008516:Medicine, Chinese Traditional; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013577:Syndrome
D051401:Aquaporin 4; D051400:Aquaporin 3