Preliminary toxicity study of dichloromethane extract of Kielmeyera coriacea stems in mice and rats.
Kielmeyera coriacea Mart. (Clusiaceae), known as "Pau Santo" or "Saco de Boi" in the central Brazilian plateau region, is used to treat several tropical diseases. The present study evaluated the toxic effects of dichloromethane (DcM) extract of Kielmeyera coriacea stems, administered to rodents. In the acute toxicity tests, mice receiving doses of this extract by the oral and intraperitoneal routes, showed reversible effects, with LD50 values of 1503.0 and 538.8 mg/kg, respectively. In the repeated-dose oral (90 days) toxicity tests, male and female Wistar rats were treated by gavage with different doses of DcM extract (5, 25 or 125 mg/kg). In biochemical and haematological evaluations, the results varied widely in respect to dose and sex, with no linear profile, and did not show clinical correlations. In the histopathological examinations, the groups exhibited some changes, but there were no significant differences between the groups compared to the controls. In conclusion, these investigations appeared to indicate the safety of acute and repeated oral administration of the DcM extract of Kielmeyera coriacea stems, which can therefore be continuously used with safety.
Obici
Simoni
S
Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá, Maringá, Paraná, Brazil. simoniobici@yahoo.com.br
Otobone
Fernanda Jacques
FJ
da Silva Sela
Vânia Ramos
VR
Ishida
Kelly
K
da Silva
José Carlos
JC
Nakamura
Celso Vataru
CV
Garcia Cortez
Diógenes Aparício
DA
Audi
Elisabeth Aparecida
EA
D000284:Administration, Oral; D000818:Animals; D001938:Brazil; D028084:Clusiaceae / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D005260:Female; D007274:Injections, Intraperitoneal; D007928:Lethal Dose 50; D008297:Male; D008519:Medicine, Traditional; D008752:Methylene Chloride / Q000737:chemistry; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage / Q000633:toxicity*; D018547:Plant Stems; D051381:Rats; D017208:Rats, Wistar; D012737:Sex Factors; D023382:Toxicity Tests, Acute
D010936:Plant Extracts; D008752:Methylene Chloride