DNA binding and biological studies of some novel water-soluble polymer-copper(II)-phenanthroline complexes.
Some novel water-soluble polymer-copper(II)-phenanthroline complex samples, [Cu(phen)2(BPEI)]Cl(2).4H2O (phen=1,10-phenanthroline, BPEI=branched polyethyleneimine), with different degrees of copper complex content in the polymer chain have been prepared by ligand substitution method in water-ethanol medium and characterized by infrared, UV-visible, EPR spectral and elemental analysis methods. The binding of these complex samples with DNA has been investigated by electronic absorption spectroscopy, emission spectroscopy and gel retardation assay. Electrostatic interactions between DNA molecule and polymer-copper(II) complex molecule containing many high positive charges have been observed. Besides these ionic interactions, van der Waals interactions, hydrogen bonding and other partial intercalation binding modes may also exist in this system. The polymer-copper(II) complex with higher degree of copper complex content was screened for its antimicrobial activity and antitumor activity.
Kumar
Rajendran Senthil
RS
Department of Chemistry, Bharathidasan University, Palkalaiperur, Tiruchirappalli 620 024, Tamil Nadu, India.
Arunachalam
Sankaralingam
S
Periasamy
Vaiyapuri Subbarayan
VS
Preethy
Christo Paul
CP
Riyasdeen
Anvarbatcha
A
Akbarsha
Mohammad Abdulkader
MA
D000042:Absorption; D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D006690:Bisbenzimidazole; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000378:metabolism*; D015195:Drug Design; D004587:Electrophoresis, Agar Gel; D005658:Fungi / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D008024:Ligands; D010618:Phenanthrolines / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D014867:Water / Q000737:chemistry*
D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D008024:Ligands; D010618:Phenanthrolines; D011108:Polymers; D014867:Water; D003300:Copper; D004247:DNA; D006690:Bisbenzimidazole