title: Rapid determination of chloramphenicol residues in aquaculture tissues by immunochromatographic assay.
abstract: An immunochromatographic assay was developed to detect chloramphenicol (CAP) residues in aquaculture tissues. The limit of detection (LOD) was 10 ng g(-1) for detecting CAP spiked in the aquaculture tissues. The results were confirmed by liquid chromatography tandem mass spectrometry (LC/MS/MS) and indicated that there was a good agreement between the two methods. The linear regression equation was y = 1.19x + 0.539 with R(2) = 0.978. The assay time for test was less than 5 min and the method is suitable for rapid testing on-site.
authors.affiliation: WuXi KangBaiEr Biotechnology Corporation, WuXi, JiangSu, P.R. China. keli81@hotmail.com
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.forename: Liqiang
authors.initials: L
authors.lastname: Liu
authors.forename: ChuanLai
authors.initials: C
authors.lastname: Xu
authors.forename: XiaoGang
authors.initials: X
authors.lastname: Chu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D000906:Antibodies / Q000276:immunology; D017756:Aquaculture; D002478:Cells, Cultured; D002701:Chloramphenicol / Q000032:analysis*; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods*; D003429:Cross Reactions / Q000276:immunology; D005260:Female; D005399:Fishes; D007118:Immunoassay / Q000379:methods*; D016014:Linear Models; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D000906:Antibodies; D002701:Chloramphenicol