title: Novel mass tags for single nucleotide polymorphism detection.
abstract: A new method suitable for single nucleotide polymorphism detection and other applications based on oligonucleotide probe extension has been developed. The method is based on mass spectrometry and utilizes a single surface for affinity purification of extended probes and matrix-independent desorption/ionization of the cleavable labels. A new family of sulfur-linked laser-cleavable trityl labels with vastly improved flying abilities is implemented in this study. Corresponding reagents compatible with automated oligonucleotide synthesis are presented. Utility of this method for SNP genotyping is demonstrated.
authors.affiliation: Shemyakin-Ovchinikov Institute of Bioorganic Chemistry, RAS, Miklukho-Maklaya 16/10, 117997, Moscow, Russia.
authors.forename: Klara R
authors.initials: KR
authors.lastname: Birikh
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Korshun
authors.forename: Pablo L
authors.initials: PL
authors.lastname: Bernad
authors.forename: Andrei D
authors.initials: AD
authors.lastname: Malakhov
authors.forename: Natalie
authors.initials: N
authors.lastname: Milner
authors.forename: Safraz
authors.initials: S
authors.lastname: Khan
authors.forename: Edwin M
authors.initials: EM
authors.lastname: Southern
authors.forename: Mikhail S
authors.initials: MS
authors.lastname: Shchepinov
mesh_terms: D004252:DNA Mutational Analysis / Q000379:methods*; D017931:DNA Primers / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D005838:Genotype; D006046:Gold / Q000737:chemistry; D013058:Mass Spectrometry; D008957:Models, Genetic; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009696:Nucleic Acids / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013499:Surface Properties
chemical_list: D017931:DNA Primers; D009696:Nucleic Acids; D006046:Gold