title: Efficient gene transfer in CLL by mRNA electroporation.
abstract: Chronic lymphocytic leukemia (CLL) consists of at least two major prognostic subgroups, characterized by different cellular and molecular markers. This observation sparked studies on the function and clinical importance of these markers. In order to address their function adequately, an efficient and reliable method for gene transfer is needed. In this study, we compared efficiency and utility of different gene transfer techniques in CLL. Lenti-, retro- and adenoviral transduction did not yield appreciable numbers of marker gene enhanced green fluorescent protein (EGFP) positive CLL cells, despite various prestimulation protocols. Efficient transgene expression was observed after nucleofection of CLL cells with plasmid DNA, at the expense of low survival rates. After optimization, electroporation of in vitro transcribed mRNA yielded up to 90% EGFP+CLL cells without affecting survival. Transgene expression remained detectable for at least 2 weeks after electroporation. Furthermore, we could demonstrate overexpression of ZAP70 and of a ZAP70-EGFP fusion protein after electroporation with ZAP70 or ZAP70-EGFP mRNA. We conclude that mRNA electroporation is a novel and straightforward method for highly efficient gene transfer in CLL. The application of this technique should facilitate functional studies on CLL cells, as well as clinical research.
authors.affiliation: Ghent University Hospital, Ghent, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Van Bockstaele
authors.forename: V
authors.initials: V
authors.lastname: Pede
authors.forename: E
authors.initials: E
authors.lastname: Naessens
authors.forename: S
authors.initials: S
authors.lastname: Van Coppernolle
authors.forename: V
authors.initials: V
authors.lastname: Van Tendeloo
authors.forename: B
authors.initials: B
authors.lastname: Verhasselt
authors.forename: J
authors.initials: J
authors.lastname: Philippé
mesh_terms: D015415:Biomarkers; D002470:Cell Survival; D002478:Cells, Cultured; D018274:Electroporation / Q000379:methods* / Q000592:standards; D015870:Gene Expression; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology*; D012333:RNA, Messenger / Q000008:administration & dosage*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D051746:ZAP-70 Protein-Tyrosine Kinase / Q000235:genetics
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D051746:ZAP-70 Protein-Tyrosine Kinase