title: [Preprocessing of collagenous sources with the aim of extracting minimally denatured collagen].
abstract: Biomedical applications of collagen are conditioned by its use in a state very close to the native state, as it concerns conformation and reactivity. Minimal denaturation implies that both the collagen structure and the side amino acid groups are kept intact. The bulk of alterations take place in phases preceding the extraction itself.
  
  
  METHODS
  The authors have established an optimal protocol for preprocessing of young bovine hides, i.e. to obtain a maximum solubilisation yield when a standard proteases-based solubilisation technique was applied.
  
  
  RESULTS
  The sequence: unhairing--loosing--cleaning--labilisation of corium tissue was taken in account. The effect of the treating composition upon the deamination degree of the extracted collagen was determined through a binary gradient HPLC technique, on a ProPac WCX-10 analytical column. The optimal range of loosing--cleaning recipe components was found to be between 18 ... 27% dithiotreitol (DDT), 12 ... 20% N-ethyl-acetamide (NEM) and 30 ... 53% iodide-acetamide (IDAM).
authors.affiliation: Facultatea de Textile - Pielărie, Catedra de Technologia chimică a produselor din piele, Universitatea Tehnică "Gh. Asachi" Iaşi.
authors.forename: S S
authors.initials: SS
authors.lastname: Maier
authors.forename: Vasilica
authors.initials: V
authors.lastname: Maier
authors.forename: Ingrid
authors.initials: I
authors.lastname: Bucişcanu
authors.forename: Melinda
authors.initials: M
authors.lastname: Pruneanu
mesh_terms: D000465:Algorithms; D000818:Animals; D002417:Cattle; D003094:Collagen / Q000096:biosynthesis*; D003641:Deamination; D010447:Peptide Hydrolases / Q000096:biosynthesis; D012867:Skin
chemical_list: D003094:Collagen; D010447:Peptide Hydrolases