Evaluating the quality of data from DNA microarray measurements.
Gene expression technology offers great potentials to generate new insights into human disease pathogenesis; however, the data quality remains a major obstacle for realizing its potentials. In the present study 60-mers oligonucleotide target immobilized on coated glass slides were utilized as a model system to investigate parameters, such as target concentration, retention, signal linearity, and fluorescence properties of fluorophores, which likely affect the quality of microarray results. An array calibration slide was used to calibrate an Axon GenePix 4000A scanner and ensure the dynamic range of the instrument. The work is a first step toward our goal of quantitative gene expression measurements.
Wang
Lili
L
Biotechnology Division, National institute of Standards and TEchnology, Gaithersburg, MD, USA.
Gaigalas
A K
AK
Satterfield
M B
MB
Salit
M
M
Zong
Y
Y
Noble
J
J
D002138:Calibration; D002232:Carbocyanines / Q000378:metabolism; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009841:Oligonucleotides; D013050:Spectrometry, Fluorescence
D002232:Carbocyanines; D009841:Oligonucleotides; C089187:cyanine dye 3; C085321:cyanine dye 5