title: DNA polymerase I is not required for replication of linear chromosomes in streptomyces.
abstract: Both polA (encoding DNA polymerase I; Pol I) and a paralog were deleted from Streptomyces strains. Despite the UV sensitivity and slow growth caused by the DeltapolA mutation, the double mutant was viable. Thus, in contrast to a previous postulate, Pol I and its paralog are not essential for replication of Streptomyces chromosomes.
authors.affiliation: Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan.
authors.forename: Tzu-Wen
authors.initials: TW
authors.lastname: Huang
authors.forename: Carton W
authors.initials: CW
authors.lastname: Chen
mesh_terms: D002876:Chromosomes, Bacterial / Q000378:metabolism*; D004256:DNA Polymerase I / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000096:biosynthesis*; D017353:Gene Deletion; D050296:Microbial Viability; D016254:Mutagenesis, Insertional; D013302:Streptomyces / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D004256:DNA Polymerase I