Oligonucleotide loading determines cellular uptake of DNA-modified gold nanoparticles.
The cellular internalization of oligonucleotide-modified nanoparticles is investigated. Uptake is dependent on the density of the oligonucleotide loading on the surface of the particles, where higher densities lead to greater uptake. Densely functionalized nanoparticles adsorb a large number of proteins on the nanoparticle surface. Nanoparticle uptake is greatest where a large number of proteins are associated with the particle.
Giljohann
David A
DA
Department of Chemistry and International Institute for Nanotechnology, Northwestern University, Evanston, IL 60208-3113, USA.
Seferos
Dwight S
DS
Patel
Pinal C
PC
Millstone
Jill E
JE
Rosi
Nathaniel L
NL
Mirkin
Chad A
CA
D001692:Biological Transport; D004247:DNA / Q000737:chemistry* / Q000493:pharmacokinetics; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000493:pharmacokinetics; D013499:Surface Properties
D009838:Oligodeoxyribonucleotides; D006046:Gold; D004247:DNA