Molecular typing of clinical isolates of Stenotrophomonas maltophilia by pulsed-field gel electrophoresis and random primer PCR fingerprinting.
During a 1-year study period, 96 strains of Stenotrophomonas maltophilia were isolated from different specimens of 59 patients admitted to a 1500 bed tertiary care university hospital. The strains were identified by commercially available API system and genotyped by pulsed-field gel electrophoresis (PFGE) and random primer PCR fingerprinting. The wide variation of the molecular profiles of the investigated S. maltophilia isolates shown by PFGE as well as PCR typing suggests that there was no outbreak situation with a particular S. maltophilia strain.
Schaumann
Reiner
R
Institute for Medical Microbiology and Epidemiology of Infectious Diseases, University of Leipzig, Liebigstr. 24, D-04103 Leipzig, Germany. reiner.schaumann@medizin.uni-leipzig.de
Laurin
Franziska
F
Rodloff
Arne C
AC
D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D016172:DNA Fingerprinting; D017931:DNA Primers; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D005858:Germany / Q000453:epidemiology; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology*; D006785:Hospitals, University; D006801:Humans; D016133:Polymerase Chain Reaction; D020615:Stenotrophomonas maltophilia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D017931:DNA Primers