title: Melatonin reduces lipid and protein oxidative damage in synaptosomes due to aluminium.
abstract: Prolonged exposure to excessive aluminium (Al) concentrations is involved in the ethiopathology of certain dementias and neurological disorders. Melatonin is a well-known antioxidant that efficiently reduces lipid peroxidation due to oxidative stress. Herein, we investigated in synaptosomal membranes the effect of melatonin in preventing Al promotion of lipid and protein oxidation when the metal was combined with FeCl(3) and ascorbic acid. Lipid peroxidation was estimated by quantifying malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) concentrations in the membrane suspension and protein carbonyls were measured in the synaptosomes as an index of oxidative damage. Under our experimental conditions, the addition of Al (0.0001-1mmol/L) enhanced MDA+4-HDA formation in the synaptosomes. In addition, Al (1mmol/L) raised protein carbonyl contents. Melatonin reduced, in a concentration-dependent manner, lipid and protein oxidation due to Al, FeCl(3) and ascorbic acid in the synaptosomal membranes. These results show that melatonin confers protection against Al-induced oxidative damage in synaptosomes and suggest that this indoleamine may be considered as a neuroprotective agent in Al toxicity because of its antioxidant activity.
authors.affiliation: Department of Pharmacology and Physiology, University of Zaragoza, Spain.
authors.forename: Carlos David
authors.initials: CD
authors.lastname: Albendea
authors.forename: Eva María
authors.initials: EM
authors.lastname: Gómez-Trullén
authors.forename: Lorena
authors.initials: L
authors.lastname: Fuentes-Broto
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Miana-Mena
authors.forename: Sergio
authors.initials: S
authors.lastname: Millán-Plano
authors.forename: Marcos César
authors.initials: MC
authors.lastname: Reyes-Gonzales
authors.forename: Enrique
authors.initials: E
authors.lastname: Martínez-Ballarín
authors.forename: Joaquín J
authors.initials: JJ
authors.lastname: García
mesh_terms: D000535:Aluminum / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015227:Lipid Peroxidation; D008055:Lipids / Q000737:chemistry*; D008550:Melatonin / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013574:Synaptosomes / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology
chemical_list: D000975:Antioxidants; D008055:Lipids; D018696:Neuroprotective Agents; D011506:Proteins; D000535:Aluminum; D008550:Melatonin