title: Photon scattering effects in optical mapping of propagation and arrhythmogenesis in the heart.
abstract: BACKGROUND
  Optical mapping is a widely used experimental tool providing high-resolution recordings of cardiac electrical activity. However, the technique is limited by signal distortion due to photon scattering in the tissue. Computational models of the fluorescence recording are capable of assessing these distortion effects, providing important insight to assist experimental data interpretation.
  
  
  METHODS
  We present results from a new panoramic optical mapping model, which is used to assess distortion in ventricular optical mapping signals during pacing and arrhythmogenesis arising from 3-dimensional photon scattering.
  
  
  CONCLUSIONS
  We demonstrate that accurate consideration of wavefront propagation within the complex ventricular structure, along with accurate representation of photon scattering in 3 dimensions, is essential to faithfully assess distortion effects arising during optical mapping. In this article, examined effects include (1) the specific morphology of the optical action potential upstroke during pacing and (2) the shift in the location of epicardial phase singularities obtained from fluorescent maps.
authors.affiliation: Computational Biology Group, University of Oxford Computing Laboratory, Oxford, UK.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Bishop
authors.forename: David J
authors.initials: DJ
authors.lastname: Gavaghan
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Trayanova
authors.forename: Blanca
authors.initials: B
authors.lastname: Rodriguez
mesh_terms: D000818:Animals; D001145:Arrhythmias, Cardiac / Q000175:diagnosis* / Q000503:physiopathology*; D018780:Body Surface Potential Mapping / Q000379:methods*; D003198:Computer Simulation; D006329:Heart Conduction System / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D008955:Models, Cardiovascular; D017785:Photons; D012542:Scattering, Radiation