title: Mutations in DIIS5 and the DIIS4-S5 linker of Drosophila melanogaster sodium channel define binding domains for pyrethroids and DDT.
abstract: Mutations in the DIIS4-S5 linker and DIIS5 have identified hotspots of pyrethroid and DDT interaction with the Drosophila para sodium channel. Wild-type and mutant channels were expressed in Xenopus oocytes and subjected to voltage-clamp analysis. Substitutions L914I, M918T, L925I, T929I and C933A decreased deltamethrin potency, M918T, L925I and T929I decreased permethrin potency and T929I, L925I and I936V decreased fenfluthrin potency. DDT potency was unaffected by M918T, but abolished by T929I and reduced by L925I, L932F and I936V, suggesting that DIIS5 contains at least part of the DDT binding domain. The data support a computer model of pyrethroid and DDT binding.
authors.affiliation: School of Biology, The University of Nottingham, University Park, Nottingham, UK. peter.usherwood@nottingham.ac.uk
authors.forename: P N R
authors.initials: PN
authors.lastname: Usherwood
authors.forename: T G E
authors.initials: TG
authors.lastname: Davies
authors.forename: I R
authors.initials: IR
authors.lastname: Mellor
authors.forename: A O
authors.initials: AO
authors.lastname: O'Reilly
authors.forename: F
authors.initials: F
authors.lastname: Peng
authors.forename: H
authors.initials: H
authors.lastname: Vais
authors.forename: B P S
authors.initials: BP
authors.lastname: Khambay
authors.forename: L M
authors.initials: LM
authors.lastname: Field
authors.forename: M S
authors.initials: MS
authors.lastname: Williamson
mesh_terms: D000818:Animals; D003634:DDT / Q000737:chemistry / Q000494:pharmacology*; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D004594:Electrophysiology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009154:Mutation / Q000235:genetics; D018408:Patch-Clamp Techniques; D011485:Protein Binding; D011722:Pyrethrins / Q000737:chemistry / Q000494:pharmacology*; D016415:Sequence Alignment; D015222:Sodium Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D011722:Pyrethrins; D015222:Sodium Channels; D003634:DDT