title: Separation behavior of electron-beam curing derived, acrylate-based monoliths.
abstract: Electron beam (EB) curing-derived monolith materials were prepared from ethyl methacrylate (EMA), trimethylolpropane triacrylate (TMPTA), 2-propanol, 1-dodecanol, and toluene within the confines of 3 mmx100 mm id glass columns, applying a total dose of 22 kGy for curing. Monolithic columns were checked for their separation behavior for selected dansylated (DNS)-amino acids as well as for cyclophilin 18. Their separation performance was compared to that of a C18-modified silica-based rigid rod (Chromoliths). In the separation of dansylated amino acids, retention times were reduced on EB-derived columns, where the peak resolution was significantly better than on a Chromolith. This finding was attributed to a larger fraction of small pores (<2.15 nm) in the EB curing-derived monoliths. Finally, EB curing-derived monoliths have been used to separate cyclophilin 18 from crude cell lysis mixtures.
authors.affiliation: Leibniz-Institut für Oberflächenmodifizierung IOM, Leipzig, Germany.
authors.forename: Rajendar
authors.initials: R
authors.lastname: Bandari
authors.forename: Christian
authors.initials: C
authors.lastname: Elsner
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Knolle
authors.forename: Christa
authors.initials: C
authors.lastname: Kühnel
authors.forename: Ulrich
authors.initials: U
authors.lastname: Decker
authors.forename: Michael R
authors.initials: MR
authors.lastname: Buchmeiser