title: Establishment, characterization, and viral susceptibility of two cell lines derived from goldfish Carassius auratus muscle and swim bladder.
abstract: Goldfish Carassius auratus are common aquarium fish and have a significant economic and research value, having considerable worth to fisheries as a baitfish and the ability to adapt to a range of habitats. Two cell lines were established from goldfish muscle and swim bladder tissue, in order to create a biological monitoring tool for viral diseases. Cell lines were optimally maintained at 30 degrees C in Leibovitz-15 medium supplemented with 20% fetal bovine serum. Propagation of goldfish cells was serum dependent, with a low plating efficiency (>16%). Karyotyping analysis indicated that both cell lines remained diploid, with a mean chromosomal count of 104. Results of viral challenge assays revealed that both cell lines shared similar patterns of viral susceptibility and production to infectious hematopoietic necrosis virus, infectious pancreatic necrosis virus, snakehead rhabdovirus, and spring viremia carp virus. Both cell lines demonstrated a higher sensitivity and significantly larger viral production than control brown bullhead cells for channel catfish virus. These newly established cell lines will be used as a diagnostic tool for viral diseases in this fish species and also for the isolation and study of goldfish viruses in the future.
authors.affiliation: Kewalo Marine Laboratory, Pacific Biosciences Research Center, University of Hawaii at Manoa, 41 Ahui Street, Honolulu, Hawaii 96813, USA.
authors.forename: Luc
authors.initials: L
authors.lastname: Rougée
authors.forename: Gary K
authors.initials: GK
authors.lastname: Ostrander
authors.forename: Robert H
authors.initials: RH
authors.lastname: Richmond
authors.forename: Yuanan
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000400:Air Sacs / Q000166:cytology / Q000821:virology; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002875:Chromosomes; D015925:Cryopreservation / Q000662:veterinary; D004198:Disease Susceptibility / Q000662:veterinary* / Q000821:virology; D005393:Fish Diseases / Q000276:immunology / Q000821:virology*; D006054:Goldfish / Q000821:virology*; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D009132:Muscles / Q000166:cytology / Q000821:virology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D013696:Temperature; D013997:Time Factors; D014777:Virus Diseases / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D014780:Viruses / Q000472:pathogenicity*
chemical_list: D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S