title: Detection of HHV-8 and HPV in laryngeal carcinoma.
abstract: OBJECTIVE
  In this study we aimed to investigate the presence of human herpesvirus 8 (HHV-8) and human papillomavirus (HPV) in laryngeal carcinoma.
  
  
  METHODS
  Fifty patients operated on because of laryngeal carcinoma were included in the study. Forty-seven had squamous cell carcinoma (SCC) whereas three had verrucous carcinoma. Fresh tumoral tissues, or tumoral tissues obtained from archival paraffin-embedded blocks, were examined. HHV-8 DNA and HPV DNA were detected using polymerase chain reaction (PCR) and viral genotypes of HPV were determined via the hybrid capture method. The presence of HHV-8 DNA and HPV DNA were also investigated in normal appearing laryngeal tissue collected from 50 cadavers at autopsy.
  
  
  RESULTS
  HPV DNA was detected in seven patients (7/50; 14%) (5 out of 47 patients with SCC (5/47; 10.6%) and two out of three patients with verrucous carcinoma). HHV-8 DNA was detected in five patients and they all had SCC (5/47; 10.6%). One case had both HHV-8 and HPV DNA. None of the control samples from cadavers harbored HHV-8 DNA, or HPV DNA. There was a statistically significant correlation between HHV-8 DNA and HPV DNA positivity and laryngeal SCC (Fisher exact test; p=0.023 for each). No statistically significant correlations were found between the presence of HHV-8 and/or HPV and age, gender, tumor stage, differentiation, the site of the tumor, smoking and alcohol use.
  
  
  CONCLUSIONS
  The findings of the present study suggest that beside HPV, HHV-8 might have a role in laryngeal carcinogenesis. Further investigations are necessary to clarify the exact role of these viruses in laryngeal carcinoma.
authors.affiliation: Istanbul University, Cerrahpaşa Medical Faculty, Department of Otorhinolaryngology, Turkey. guvencmg@yahoo.com
authors.forename: Melih Güven
authors.initials: MG
authors.lastname: Güvenç
authors.forename: Kenan
authors.initials: K
authors.lastname: Midilli
authors.forename: Ahmet
authors.initials: A
authors.lastname: Ozdoğan
authors.forename: Ender
authors.initials: E
authors.lastname: Inci
authors.forename: Rauf
authors.initials: R
authors.lastname: Tahamiler
authors.forename: Ozgün
authors.initials: O
authors.lastname: Enver
authors.forename: Gözde
authors.initials: G
authors.lastname: Sirin
authors.forename: Sevgi
authors.initials: S
authors.lastname: Ergin
authors.forename: Mert
authors.initials: M
authors.lastname: Kuşkucu
authors.forename: Emel Ozergil
authors.initials: EO
authors.lastname: Divanoğlu
authors.forename: Gülden
authors.initials: G
authors.lastname: Yilmaz
authors.forename: Kemal
authors.initials: K
authors.lastname: Altas
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D018289:Carcinoma, Verrucous / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005838:Genotype; D019288:Herpesvirus 8, Human / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology / Q000821:virology*; D007830:Larynx / Q000473:pathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral