Cloning of a putative hypersensitive induced reaction gene from wheat infected by stripe rust fungus.
The hypersensitive response (HR) is one of the most efficient forms of plant defense against biotrophic pathogens and results in localized cell death and the formation of necrotic lesions. In this study, a novel putative hypersensitive induced reaction (HIR) gene from wheat leaves infected by incompatible stripe rust pathogen CY23, designated as Ta-hir1, was identified by using rapid amplification of cDNA ends (RACE). Ta-hir1 encodes 284 amino acids, with a predicted molecular mass of 31.31 KDa. A phylogenetic analysis showed that Ta-hir1 was highly homologous to Hv-hir1 from barley at both cDNA and deduced amino-acid levels. Amino-acid sequence analysis of the wheat HIR protein indicated the presence of the SPFH (Stomatins, Prohibitins, Flotillins and HflK/C) protein domain typical for stomatins which served as a negative regulator of univalent cation permeability, especially for potassium. The expression profile of the Ta-hir1 transcript detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time polymerase chain reaction (real time-PCR), respectively, showed that the highest expression occurred 48 h post inoculation (hpi), which is consistent with our previous histopathology observations during the stripe rust fungus-wheat incompatible reaction.
Yu
Xiu-Mei
XM
College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A and F University, Yangling, Shaanxi, PR China.
Yu
Xiu-Dao
XD
Qu
Zhi-Peng
ZP
Huang
Xin-Jie
XJ
Guo
Jun
J
Han
Qing-Mei
QM
Zhao
Jie
J
Huang
Li-Li
LL
Kang
Zhen-Sheng
ZS
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005658:Fungi / Q000472:pathogenicity; D015870:Gene Expression; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D014908:Triticum / Q000235:genetics* / Q000382:microbiology
D001425:Bacterial Outer Membrane Proteins; D018076:DNA, Complementary; C075462:harpin protein, Erwinia amylovora