title: Designing Zn(II) and Cu(II) derivatives as probes for in vitro fluorescence imaging.
abstract: New M(II) bis(thiosemicarbazonato) complexes (M = Ni(II), Cu(II) and Zn(II)) featuring allyl groups at the exocyclic nitrogens have been synthesised. The complexes were characterised in solution by spectroscopic methods and their solid state structures determined by single crystal X-ray diffraction using synchrotron radiation. The Zn(II) complex was found to be intrinsically fluorescent and soluble in biocompatible media. The uptake of this Zn(II) complex in HeLa, MCF-7 and IGROV cancer cells was monitored by fluorescence microscopies (epi- and confocal fluorescence imaging). The radiolabelling to (64)Cu(II) bis(thiosemicarbazonato) complex was performed cleanly by transmetallation from the corresponding Zn(II) species using (64)Cu(OAc)(2).
authors.affiliation: Chemistry Research Laboratory, Universiy of Oxford, 12 Mansfield Road, Oxford, UKOX1 3TA. sofia.pascu@chem.ox.ac.uk
authors.forename: Sofia I
authors.initials: SI
authors.lastname: Pascu
authors.forename: Philip A
authors.initials: PA
authors.lastname: Waghorn
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Conry
authors.forename: Helen M
authors.initials: HM
authors.lastname: Betts
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Dilworth
authors.forename: Grant C
authors.initials: GC
authors.lastname: Churchill
authors.forename: Tzveta
authors.initials: T
authors.lastname: Pokrovska
authors.forename: Martin
authors.initials: M
authors.lastname: Christlieb
authors.forename: Franklin I
authors.initials: FI
authors.lastname: Aigbirhio
authors.forename: John E
authors.initials: JE
authors.lastname: Warren
mesh_terms: D045744:Cell Line, Tumor; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008856:Microscopy, Fluorescence / Q000379:methods*; D015335:Molecular Probes; D015032:Zinc / Q000737:chemistry*
chemical_list: D015335:Molecular Probes; D003300:Copper; D015032:Zinc