title: Fresh-wet storage accelerates aortic homograft calcification.
abstract: The studies were focused on the influence of the fresh-wet storage procedure on aortic valve homograft (AVH) mineralization. Thirty-four aortic valves excised at autopsy were investigated. The cusps were divided into halves, the first one was used as a control while the second half was stored in Morgan, Morton, and Parker's Medium 199 supplemented with an antibiotic mixture at 4 degrees C for 28 days. The elemental compositions of the samples were determined by the energy dispersive X-ray fluorescence method. The Ca/P ratio and Ca concentration was used as markers of the mineralization development. It was found that the AVH mineralization was accelerated by the applied fresh-wet storage procedure. The aggravation of the AVH mineralization was correlated with the Ca content before storage especially in case of old donors. For donors older than 40 years an increase of Ca concentrations by approximately 40% was observed. To limit AVH mineralization due to fresh-wet storage, age of donors should not exceed approximately 40 years. Another method to reduce AVH mineralization relies on a modification of the medium commonly applied in the fresh-wet storage procedure, and research are ongoing.
authors.affiliation: Department of Cardiac and Vascular Diseases, Jagiellonian University, Medical College, Pradnicka 80, 31-202 Krakow, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Podolec
authors.forename: Rafal
authors.initials: R
authors.lastname: Drwiła
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Goncerz
authors.forename: Eugeniusz
authors.initials: E
authors.lastname: Rokita
authors.forename: Jerzy
authors.initials: J
authors.lastname: Sadowski
authors.forename: Wieslawa
authors.initials: W
authors.lastname: Tracz
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wróbel
mesh_terms: D000367:Age Factors; D001021:Aortic Valve / Q000737:chemistry / Q000473:pathology* / Q000637:transplantation*; D002114:Calcinosis / Q000473:pathology*; D002118:Calcium / Q000032:analysis; D006801:Humans; D009926:Organ Preservation / Q000379:methods*; D010758:Phosphorus / Q000032:analysis; D013048:Specimen Handling; D013052:Spectrometry, X-Ray Emission; D013997:Time Factors; D014019:Tissue Donors; D014184:Transplantation, Homologous
chemical_list: D010758:Phosphorus; D002118:Calcium