Development and initial application of a hybridization-independent, DNA-encoded reaction discovery system compatible with organic solvents.
We have developed and applied an approach to reaction discovery that takes advantage of DNA encoding, DNA-programmed assembly of substrate pairs, in vitro selection, and PCR amplification, yet does not require reaction conditions that support DNA hybridization. This system allows the simultaneous evaluation of >200 potential bond-forming combinations of substrates in a single experiment and can be applied in a range of solvent and temperature conditions. In an initial application, we applied this system to explore Au(III)-mediated chemistry and uncovered a simple, mild method for the selective Markovnikov-type hydroarylation of vinyl arenes and trisubstituted olefins with indoles.
Rozenman
Mary M
MM
Howard Hughes Medical Institute and the Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Kanan
Matthew W
MW
Liu
David R
DR
D000143:Acids / Q000737:chemistry; D002384:Catalysis; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry; D015394:Molecular Structure; D020411:Oligonucleotide Array Sequence Analysis; D012997:Solvents / Q000737:chemistry*; D020058:Styrene / Q000737:chemistry*; D013696:Temperature
D000143:Acids; D015342:DNA Probes; D007211:Indoles; D012997:Solvents; D020058:Styrene; D004247:DNA