Inhibitory effect of eupatilin and jaceosidin isolated from Artemisia princeps in IgE-induced hypersensitivity.
To understand the antiallergic effect of Artemisia princeps (AP), which has been found to show inhibitory activity against degranulation and a passive cutaneous anaphylaxis (PCA) reaction, eupatilin and jaceosidin, as the active components, were isolated by degranulation-inhibitory activity-guided fractionation, with their antiallergic activity investigated. These isolated components potently inhibited the release of beta-hexosaminidase from RBL-2H3 cells induced by the IgE-antigen complex, with IC(50) values of 3.4 and 4.5muM, respectively. Eupatilin and jaceosidin potently inhibited the PCA reaction and scratching behaviors induced by IgE- antigen complex and compound 48/80, respectively. Orally administered jaceosidin more potently inhibited the PCA reaction than that of eupatilin, although the PCA reaction-inhibitory activity of intraperitoneally administered jaceosidin was nearly the same as that of eupatilin. Eupatilin and jaceosidin inhibited the gene expressions of TNF-alpha and IL-4 in RBL-2H3 cells stimulated by IgE-antigen complex. Eupatilin and jaceosidin inhibited the activation of NF-kB. Based on these findings, eupatilin and jaceosidin may be useful for protection from the PCA and itching reactions, which are IgE-mediated representative skin allergic diseases.
Lee
Seung Hoon
SH
College of Pharmacy, Kyung-Hee University, 1, Hoegi, Dongdaemun-ku, Seoul 130-701, South Korea.
Bae
Eun-Ah
EA
Park
Eun-Kyung
EK
Shin
Yong-Wook
YW
Baek
Nam-In
NI
Han
Eun-Joo
EJ
Chung
Hae-Gon
HG
Kim
Dong-Hyun
DH
D000818:Animals; D019306:Artemisia / Q000737:chemistry*; D016207:Cytokines / Q000096:biosynthesis; D005419:Flavonoids / Q000494:pharmacology*; D006967:Hypersensitivity / Q000188:drug therapy*; D007073:Immunoglobulin E / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000378:metabolism; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D016207:Cytokines; D005419:Flavonoids; D016328:NF-kappa B; C477508:jaceosidin; D007073:Immunoglobulin E; C045325:eupatilin; D001619:beta-N-Acetylhexosaminidases