title: [Establishment, optimization and application of a screening method for immunoregulatory oligodeoxynucleotides].
abstract: OBJECTIVE
  To establish a set of reasonable and convenient experimental system to provide a screening method for the development of novel immunoregulatory oligodeoxynucleotides.
  
  
  METHODS
  The human PBMCs were stimulated by CpG ODN and/or immunoregulatory ODN. The cell proliferation and anti-viral activity of the supernatant induced by CpG ODN were examined by thymidine incorporation and anti-viral bioassay to evaluate the immunoregulatory activity of candidate ODN. The experimental conditions were also optimized.
  
  
  RESULTS
  A screening method on which A151, a positive immunoregulatory ODN, inhibited the proliferation and anti-viral activity of the CpG ODN-induced human PBMCs was successfully established.
  
  
  CONCLUSIONS
  The successful establishment of CpG ODN based screening method lays the foundations for further development of novel immunoregulatory oligodeoxynucleotides.
authors.affiliation: Department of Immunology, Basic Medical College of Jilin University, Changchun 130021, China.
authors.forename: Da-Li
authors.initials: DL
authors.lastname: Hu
authors.forename: Xue-Song
authors.initials: XS
authors.lastname: Zhang
authors.forename: Hua-Ying
authors.initials: HY
authors.lastname: Wang
authors.forename: Ran
authors.initials: R
authors.lastname: Sun
authors.forename: Ji-Ling
authors.initials: JL
authors.lastname: Ren
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Wang
authors.forename: Yong-Li
authors.initials: YL
authors.lastname: Yu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D049109:Cell Proliferation; D018899:CpG Islands / Q000235:genetics; D004306:Dose-Response Relationship, Immunologic; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000032:analysis* / Q000235:genetics / Q000276:immunology*; D013997:Time Factors; D018116:Vesiculovirus / Q000276:immunology
chemical_list: D009838:Oligodeoxyribonucleotides