title: Resistive exercise blunts LPS-stimulated TNF-alpha and Il-1 beta.
abstract: To examine the influence of acute resistive exercise and "hormone status" on cytokine profile, 35 postmenopausal women (72 +/- 6.2 yr) underwent a moderate-high-intensity resistive exercise bout or rested. There were 4 groups: no hormone replacement (NHR, n = 9), hormone replacement (HRT, n = 12), selective estrogen receptor modulator (SER, n = 7), or resting control (no hormone replacement, CON, n = 7). NHR, HRT, and SER exercised (3 sets, 10 exercises @ 80 % 1RM). Blood was collected pre-exercise (PR), postexercise (PO), and two hours (2H) postexercise (same times for CON). Blood was diluted 1 : 10 in culture medium and incubated (37 degrees C, 5 % CO2, 24 h) with lipopolysaccharide (LPS, 25 microg . ml (-1)). Serum and supernatant from LPS-stimulated blood were analyzed for IL-6, IL-1 beta, and TNF-alpha using ELISA. Resistive exercise increased PO serum IL-6, and PO LPS-stimulated IL-6 and IL-1 beta in the exercise groups (HRT, NHR, SER collapsed; EX, n = 28). LPS-stimulated IL-1 beta remained elevated at 2H in EX and was significantly higher than PR in CON at 2H. Expressed per monocyte, EX had significantly lower IL-1 beta and TNF-alpha LPS-stimulated production at PO and 2H compared to CON, indicating an exercise-induced blunting of an apparent diurnal response on cytokine production. In postmenopausal women, acute resistive exercise increased circulating IL-6, but reversed an apparent diurnal increase in LPS-stimulated IL-1 beta and TNF-alpha production with no influence of hormone replacement or raloxifene.
authors.affiliation: Department of Kinesiology, Texas Christian University, Fort Worth, TX 76123, USA. m.phillips@tcu.edu
authors.forename: M D
authors.initials: MD
authors.lastname: Phillips
authors.forename: M G
authors.initials: MG
authors.lastname: Flynn
authors.forename: B K
authors.initials: BK
authors.lastname: McFarlin
authors.forename: L K
authors.initials: LK
authors.lastname: Stewart
authors.forename: K L
authors.initials: KL
authors.lastname: Timmerman
authors.forename: H
authors.initials: H
authors.lastname: Ji
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D015850:Interleukin-6 / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D008070:Lipopolysaccharides / Q000097:blood / Q000494:pharmacology*; D017698:Postmenopause; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D014481:United States; D014891:Weight Lifting / Q000502:physiology*
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha