title: [Evaluation and clinical significance of HBV large protein (LHBs) in diagnosis of hepatitis B].
abstract: OBJECTIVE
  To explore the significance of HBV large protein (LHBs) in diagnosis of hepatitis B, we detected the LHBs, HBV DNA, PreS1 and other hepatitis B viral markers (HBV M) in the serum of patients infected with HBV.
  
  
  METHODS
  HBV DNA was quantitatively detected using RT-PCR, LHBs, PreS1 and HBV M were analyzed by ELISA in totally 385 serum samples.
  
  
  RESULTS
  There was a significant difference between the positive rate of LHBs (86.97%) and PreS1 (49.5%) in the 307 serum positive for HBV DNA (P less than 0.05). There was a correlation between the levels of LHBs and the logarithm of HBV DNA (r=0.935). In the serum specimens of patients negative for HBeAg, there was no significant difference between the positive rate of LHBs (76.92%) and the HBV DNA (67.95%), but the positive rate of PreS1 (45.73%) was lower than that of LHBs or HBV DNA.
  
  
  CONCLUSIONS
  There was a close correlation between the copies of HBV DNA and the levels of LHBs, both the positive rate and the coincidence rate of LBHs and HBV DNA were higher than those of PreS1. LHBs can reflect the replication status of HBV.
authors.affiliation: Clinical Laboratory Center of Zhejiang Provincial People's Hospital, Hangzhou, China.
authors.forename: Yong-Jian
authors.initials: YJ
authors.lastname: Chen
authors.forename: Yong-Lie
authors.initials: YL
authors.lastname: Zhou
authors.forename: Jun
authors.initials: J
authors.lastname: Xia
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Xia
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D005260:Female; D006509:Hepatitis B / Q000097:blood* / Q000175:diagnosis*; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011498:Protein Precursors / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014763:Viral Matrix Proteins / Q000097:blood; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D014763:Viral Matrix Proteins; C050806:presurface protein 1, hepatitis B surface antigen