title: Microsystems technology and biosensing.
abstract: This review addresses the recent developments in miniaturized microsystems or lab-on-a-chip devices for biosensing of different biomolecules: DNA, proteins, small molecules, and cells, especially at the single-molecule and single-cell level. In order to sense these biomolecules with sensitivity we have fabricated chip devices with respect to the biomolecule to be analyzed. The details of the fabrication are also dealt with in this review. We mainly developed microarray and microfluidic chip devices for DNA, protein, and cell analyses. In addition, we have introduced the porous anodic alumina layer chip with nanometer scale and gold nanoparticles for label-free sensing of DNA and protein interactions. We also describe the use of microarray and microfluidic chip devices for cell-based assays and single-cell analysis in drug discovery research.
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi City, Ishikawa, 923-1292, Japan.
authors.forename: Ramachandra Rao
authors.initials: RR
authors.lastname: Sathuluri
authors.forename: Shohei
authors.initials: S
authors.lastname: Yamamura
authors.forename: Eiichi
authors.initials: E
authors.lastname: Tamiya
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004247:DNA / Q000032:analysis; D004566:Electrodes; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D016062:Porosity; D011506:Proteins / Q000032:analysis
chemical_list: D011506:Proteins; D004247:DNA; D000537:Aluminum Oxide