Single lymphocyte analysis with a microwell array chip.
Following genomics and proteomics, cytomics, a novel method of looking at life, has emerged for analyzing large populations of cells on a single-cell basis with multiple parameters in a quantitative manner. We have developed a highly integrated live-cell microarray system for analyzing the cellular responses of individual cells using a microwell array chip that has 234,000 microwells each of which is just large enough to fit a single cell. Compared with flow cytometry and microscope-based methods, our system can analyze the history of the cellular responses of a large number of cells. We have successfully applied the system to analyze human antigen-specific B-cells and produced human monoclonal antibodies (MoAb) against hepatitis B virus surface antigen. We have also constructed a mouse system to assess hepatitis B virus-neutralization activity and have demonstrated the neutralization activity of our antibodies. Our technology should expand the horizons of cell analysis as well as enable generation of human MoAb for antibody-based therapeutics and diagnosis for infectious diseases such as hepatitis viruses.
Tokimitsu
Yoshiharu
Y
Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
Kishi
Hiroyuki
H
Kondo
Sachiko
S
Honda
Ritsu
R
Tajiri
Kazuto
K
Motoki
Kazumi
K
Ozawa
Tatsuhiko
T
Kadowaki
Shinichi
S
Obata
Tsutomu
T
Fujiki
Satoshi
S
Tateno
Chise
C
Takaishi
Hideki
H
Chayama
Kazuaki
K
Yoshizato
Katsutoshi
K
Tamiya
Eiichi
E
Sugiyama
Toshiro
T
Muraguchi
Atsushi
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods; D006509:Hepatitis B / Q000276:immunology / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000097:blood / Q000276:immunology; D017325:Hepatitis B Vaccines / Q000097:blood / Q000276:immunology; D019694:Hepatitis B, Chronic / Q000276:immunology; D022781:Hepatocytes / Q000276:immunology / Q000821:virology; D006801:Humans; D008213:Lymphocyte Activation; D051379:Mice; D016513:Mice, SCID; D008822:Mice, Transgenic; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*
D000911:Antibodies, Monoclonal; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines