title: Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.).
abstract: Peroxidase (POD) and superoxide dismutase (SOD) enzyme activities were analyzed in non-regenerative transformed embryogenic lines of alfalfa (Medicago sativa L.) carrying wound-inducible oryzacystatin I (OC-I), wound-inducible oryzacystatin I antisense (OC-Ias), or hygromycin phosphotransferase (hpt) genes. All of the transformed lines analyzed had elevated levels of all POD isoforms. Three POD isoforms with pI values of approximately 4.5, 4.8, and 8.4, and one additional pair of isoforms with a pI value of approximately 8.8 were separated from tissue extracts of all transgenic lines. Isoelectrofocusing patterns revealed the induction of one isoform of SOD with a pI of about 5.6 in all transgenic lines compared with non-transformed embryogenic tissue. These results indicate that the process of transformation may disrupt redox homeostasis in alfalfa tissues.
authors.affiliation: Center for Multidisciplinary Studies, University of Belgrade, Kneza Viseslava 1a, 11030 Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Platisa
authors.forename: Sonja
authors.initials: S
authors.lastname: Veljović-Jovanović
authors.forename: Biljana
authors.initials: B
authors.lastname: Kukavica
authors.forename: Branka
authors.initials: B
authors.lastname: Vinterhalter
authors.forename: Ann
authors.initials: A
authors.lastname: Smigocki
authors.forename: Slavica
authors.initials: S
authors.lastname: Ninković
mesh_terms: D018533:Biomass; D004351:Drug Resistance; D047108:Embryonic Development; D004790:Enzyme Induction; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000378:metabolism; D000455:Medicago sativa / Q000196:embryology* / Q000201:enzymology* / Q000235:genetics; D010544:Peroxidases / Q000096:biosynthesis*; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D011748:Pyrogallol / Q000378:metabolism; D016372:RNA, Antisense; D012984:Software; D013379:Substrate Specificity; D013482:Superoxide Dismutase / Q000096:biosynthesis*; D014020:Tissue Extracts; D014170:Transformation, Genetic
chemical_list: D007527:Isoenzymes; D010940:Plant Proteins; D016372:RNA, Antisense; D014020:Tissue Extracts; D011748:Pyrogallol; D010544:Peroxidases; D013482:Superoxide Dismutase