Longitudinal study of meningococcal carrier rates in teenagers.
To gain actual information concerning the oropharyngeal carriage of Neisseria meningitidis among teenagers aged 15-18 years in Germany especially in a region with increased incidence of meningococal-related diseases prompted the study. Each teenager was swabbed three times with an interval of 2 months between the examinations. The 901 recovered N. meningitidis strains were characterized using serological (serogrouping, serotyping/serosubtyping) and molecular methods (PCR, PFGE) each. The results of the study demonstrate an overall average carrier rate of 18.8% for the three collection periods. There were, however, significant differences between the carrier rates within a given school and of different towns and counties. Of all isolates, 60.6% were not serogroupable. Serogroup B dominated (12.3%), followed by serogroup Y (9.0%) and serogroup C (3.6%). After PCR-based serogrouping of not serogroupable strains the percentages for serogroups enhanced to 18.8% for B, 10.8% for Y and 4.1% for C. Serotyping led to 305 different phenotypes with the most common being 29E:NT:P1.2,5 followed by Y:14:NST. In the 6 study towns the number of different N. meningitidis clones (PFGE types) isolated, varied between 30 and 87. In Wenden, where a prolonged outbreak had taken place, serogroup C (14.8%) was predominant. Only in this town C:2a isolates were found, all belonging to the ST-11/ET-37 complex and 12/13 matched identically to the ET-15 clone. Of the colonized teenagers, 26.7% were carriers over at least 23 weeks, 22.6% with the same strain, 36.0% were carrier for at least 15 weeks. Over all three collection periods 36.7% of the adolescents acquired a new strain. The highest acquisition rate was related to PFGE type 12.
Glitza
I C
IC
Abt. Hygiene und Med. Mikrobiologie, Hygiene-Institut, Universität Heidelberg, Im Neuenheimer Feld 346 (Dekanat), D-69120 Heidelberg, Germany.
Ehrhard
I
I
Müller-Pebody
B
B
Reintjes
R
R
Breuer
T
T
Ammon
A
A
Sonntag
H-G
HG
D000293:Adolescent; D002353:Carrier State / Q000453:epidemiology* / Q000382:microbiology; D005843:Geography; D005858:Germany / Q000453:epidemiology; D006801:Humans; D008137:Longitudinal Studies; D008589:Meningococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D009345:Neisseria meningitidis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009960:Oropharynx / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012574:Schools; D012703:Serotyping; D011795:Surveys and Questionnaires