Inhibition of beta-amyloid precursor protein gene in SK-N-SH cells by piperlonguminine/dihydropiperlonguminine components separated from Chinese herbal medicine Futokadsura stem.
Alzheimer disease (AD) is a progressive neurodegenerative disease characterized by progressive cognitive and memory decline. Amyloid precursor protein (APP) is a transmembrane protein, it has been known to play an important role in AD pathogenesis. Previous studies have shown that a Chinese herb Futokadsura stem can selectively inhibit the expression of amyloid precursor protein (APP) gene. We want to find the effective components in Futokadsura stem which have the inhibitory effect. Futokadsura stem was separated and purified with chemical methods, and then different separation components were added on SK-N-SH cells in different concentrations. Using MTT methods, we detected proliferation activity of SK-N-SH cells which were treated with different separation components of Futokadsura stem. Using RT-PCR, Western blot methods, we detected APP gene expression in SK-N-SH cells after they are treated with different Futokadsura stem separation components. We found that piperlonguminine/dihydropiperlonguminine components (1:0.8) separated from Futokadsura stem acetic ether extracts could selectively inhibit the expression of APP gene in SK-N-SH cells in mRNA and protein levels. This inhibition effect is concentration-dependent. Under experimental concentrations, the components did not affect the proliferation activity of SK-N-SH cells. These data suggest that piperlonguminine/dihydropiperlonguminine components are the effective components in Futokadsura stem which can inhibit the expression of APP gene.
Xia
Wen
W
Department of Cerebrovascular Disease, Qilu Hospital, Shandong University, Jinan 250012, Shandong, People's Republic of China.
Zeng
Ji-Ping
JP
Chen
Lian-Bi
LB
Jiang
An-Li
AL
Xiang
Lan
L
Xu
Jun
J
Cui
Xing
X
Han
En-Ji
EJ
D000544:Alzheimer Disease; D016564:Amyloid beta-Protein Precursor / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004148:Dioxolanes / Q000737:chemistry / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D009447:Neuroblastoma; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D031701:Piper / Q000737:chemistry*; D018514:Plant Structures / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D010881:Piperidones
D016564:Amyloid beta-Protein Precursor; D004148:Dioxolanes; D004365:Drugs, Chinese Herbal; D006573:Heterocyclic Compounds, 1-Ring; C498077:piperlongumine; D010881:Piperidones