title: Changes of biomarkers with oral exposure to benzo(a)pyrene, phenanthrene and pyrene in rats.
abstract: Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants present in air and food. Among PAHs, benzo(a)pyrene(BaP), phenanthrene (PH) and pyrene (PY) are considered to be important for their toxicity or abundance. To investigate the changes of biomarkers after PAH exposure, rats were treated with BaP (150 microg/kg) alone or with PH (4,300 microg/kg) and PY (2,700 microg/kg) (BPP group) by oral gavage once per day for 30 days. 7-ethoxyresorufin-O-deethylase activity in liver microsomal fraction was increased in only BaP groups. The highest concentration (34.5 ng/g) of BaP, was found in muscle of rats treated with BaP alone at 20 days of treatment; it was 23.6 ng/g in BPP treated rats at 30 days of treatment. The highest PH concentration was 47.1 ng/g in muscle and 118.8 ng/g in fat, and for PY it was 29.7 ng/g in muscle and 219.9 ng/g in fat, in BPP groups. In urine, 114-161 ng/ml 3-OH-PH was found, while PH was 41-69 ng/ml during treatment. 201-263 ng/ml 1-OH-PY was found, while PH was 9-17 ng/ml in urine. The level of PY, PH and their metabolites in urine was rapidly decreased after withdrawal of treatment. This study suggest that 1-OH-PY in urine is a sensitive biomarker for PAHs; it was the most highly detected marker among the three PAHs and their metabolites evaluated during the exposure period and for 14 days after withdrawal.
authors.affiliation: National Veterinary Research and Quarantine Service, Anyang 430-824, Korea.
authors.forename: Hwan Goo
authors.initials: HG
authors.lastname: Kang
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Jeong
authors.forename: Myung Haing
authors.initials: MH
authors.lastname: Cho
authors.forename: Joon Hyoung
authors.initials: JH
authors.lastname: Cho
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry / Q000187:drug effects; D000818:Animals; D001564:Benzo(a)pyrene / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*; D015415:Biomarkers / Q000378:metabolism / Q000652:urine; D001774:Blood Chemical Analysis; D001835:Body Weight / Q000187:drug effects; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004785:Environmental Pollutants / Q000097:blood / Q000378:metabolism / Q000633:toxicity* / Q000652:urine; D005260:Female; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010616:Phenanthrenes / Q000097:blood / Q000378:metabolism / Q000633:toxicity* / Q000652:urine; D011721:Pyrenes / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D015415:Biomarkers; D004785:Environmental Pollutants; D010616:Phenanthrenes; D011721:Pyrenes; D001564:Benzo(a)pyrene; C031181:phenanthrene; C030984:pyrene; D019363:Cytochrome P-450 CYP1A1