title: Chemical composition, antimicrobial and antioxidant activities of the essential oil of Tibetan herbal medicine Dracocephalum heterophyllum Benth.
abstract: The essential oil of Tibetan medicine Dracocephalum heterophyllum Benth was obtained by hydrodistillation with a 0.7% (v/w) yield. The chemical composition of the essential oil was analyzed by gas chromatography-mass spectral (GC-MS). Eighty-three compounds, constituting about 89.83% of the total oil, were identified. The main compound in the oil were Cineole (14.89%), trans-nerolido (7.10%), 1-m-ethyl-2-(1-methylethyl)-benzene (4.42%), Germacrene-D (4.84%), Decahydro-1,1,4,7-tetramethyl-4aH-cycloprop[e]azulen-4a-ol (4.94%), p-menth-1-en-4-ol,acetate (4.34%), 4-methyl-1-(1-methylethyl)-3-cyclohexen-1-ol (4.10%). The antimicrobial activity of the oil was evaluated against nine bacterial, one yeast, and three fungi. The antimicrobial test result showed that the essential oil strongly inhibited the growth of test microorganisms studied. The maximal inhibition zones and MIC values for bacterial, yeast and fungi strain were in the range of 18-25 mm and 0.039-0.156 mg mL(-1); *20 mm, and 0.156 mg mL(-1); 8-24 mm and 0.313-2.5 mg mL(-1); respectively. The antioxidant activity of the oil was determined by the malonyldialdehyde (MDA) test, measuring the MDA concentration in mouse liver cell microsomal after induced lipid peroxidation using FeSO(4) and ascorbic acid, The inhibition of lipid peroxidation was 59.3% with a concentration of 0.5 mg mL(-1). Result presented here may suggest that the essential oil of D. heterophyllum posses antimicrobial and antioxidant properties, and therefore, they can be one of new medicinal resources for antimicrobial agent and/or used as a natural preservative ingredient in food and cosmetics and pharmaceuticals industry.
authors.affiliation: School of Life Science, Lanzhou University, Lanzhou, China.
authors.forename: Chunjiang
authors.initials: C
authors.lastname: Zhang
authors.forename: Hongyu
authors.initials: H
authors.lastname: Li
authors.forename: Tian
authors.initials: T
authors.lastname: Yun
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Fu
authors.forename: Chunmei
authors.initials: C
authors.lastname: Liu
authors.forename: Bu
authors.initials: B
authors.lastname: Gong
authors.forename: Benjia
authors.initials: B
authors.lastname: Neng
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D029001:Herbal Medicine; D019686:Lamiaceae / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology; D008517:Phytotherapy; D010938:Plant Oils / Q000737:chemistry* / Q000494:pharmacology*; D018609:Tibet
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D009822:Oils, Volatile; D010938:Plant Oils