title: Genome and polypeptides characterization of Tellina virus 1 reveals a fifth genetic cluster in the Birnaviridae family.
abstract: We characterized tellina virus 1 (TV-1), a birnavirus isolated from the marine bivalve mollusk Tellina tenuis. Genome sequence analysis established that TV-1 is representative of a viral cluster distant from other birnaviruses. The maturation process of the polyprotein encoded by the genomic segment A was delineated with the identification of the N-termini of the viral protease VP4 and the ribonucleoprotein VP3, and the characterization of peptides deriving from the processing of pVP2, the VP2 capsid protein precursor. One of these peptides was shown to possess a membrane-disrupting domain. Like the blotched snakehead virus, the polyprotein exhibits a non-structural polypeptide (named [X]) located between pVP2 and VP4. Mutagenesis analysis allowed the identification in VP4 of a catalytic Ser-Lys dyad that does not possess the common Gly-X-Ser signature of the serine hydrolases. The genomic segment B encodes the viral RNA-dependent RNA-polymerase VP1 with the unique sequence motif arrangement identified in other birnavirus VP1s.
authors.affiliation: INRA, Unité de Virologie et Immunologie moléculaires UR892, Domaine de Vilvert, 78350 Jouy-en-Josas, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Nobiron
authors.forename: Marie
authors.initials: M
authors.lastname: Galloux
authors.forename: Celine
authors.initials: C
authors.lastname: Henry
authors.forename: Corinne
authors.initials: C
authors.lastname: Torhy
authors.forename: Pierre
authors.initials: P
authors.lastname: Boudinot
authors.forename: Nathalie
authors.initials: N
authors.lastname: Lejal
authors.forename: Bruno
authors.initials: B
authors.lastname: Da Costa
authors.forename: Bernard
authors.initials: B
authors.lastname: Delmas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019201:Aquabirnavirus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D018059:Birnaviridae / Q000145:classification* / Q000235:genetics; D049872:Bivalvia / Q000821:virology*; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D016679:Genome, Viral; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020815:Polyproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D010455:Peptides; D020815:Polyproteins; D014764:Viral Proteins; D012697:Serine Endopeptidases; C414992:VP4 protease, birnavirus