title: Phylogenetic analysis of vibrios and related species by means of atpA gene sequences.
abstract: We investigated the use of atpA gene sequences as alternative phylogenetic and identification markers for vibrios. A fragment of 1322 bp (corresponding to approximately 88% of the coding region) was analysed in 151 strains of vibrios. The relationships observed were in agreement with the phylogeny inferred from 16S rRNA gene sequence analysis. For instance, the Vibrio cholerae, Vibrio halioticoli, Vibrio harveyi and Vibrio splendidus species groups appeared in the atpA gene phylogenetic analyses, suggesting that these groups may be considered as separate genera within the current Vibrio genus. Overall, atpA gene sequences appeared to be more discriminatory for species differentiation than 16S rRNA gene sequences. 16S rRNA gene sequence similarities above 97% corresponded to atpA gene sequences similarities above 80%. The intraspecies variation in the atpA gene sequence was about 99% sequence similarity. The results showed clearly that atpA gene sequences are a suitable alternative for the identification and phylogenetic study of vibrios.
authors.affiliation: Department of Genetics, Federal University of Rio de Janeiro, Brazil.|Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
authors.forename: Cristiane C
authors.initials: CC
authors.lastname: Thompson
authors.affiliation: Department of Genetics, Federal University of Rio de Janeiro, Brazil.
authors.forename: Fabiano L
authors.initials: FL
authors.lastname: Thompson
authors.affiliation: Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
authors.forename: Ana Carolina P
authors.initials: ACP
authors.lastname: Vicente
authors.affiliation: Laboratory of Microbiology and BCCM/LMG Bacteria Collection, Ghent University, Belgium.
authors.forename: Jean
authors.initials: J
authors.lastname: Swings
mesh_terms: D025243:Bacterial Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014733:Vibrio / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D014737:Vibrionaceae / Q000145:classification* / Q000201:enzymology / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D025243:Bacterial Proton-Translocating ATPases