Technical modification of unilateral lung transplantation in rats.
The increasing demand in transplantation research requires efficient and less expensive animal models in order to obtain reliable results that are reproducible in larger animal models and, ultimately, applied clinically. The model of unilateral left lung transplantation in rats has proven to be a useful alternative for those purposes. We demonstrate a technical modification of this model, which consists of the isolation and ligation of the contralateral (right) pulmonary artery, allowing blood circulation exclusively in the transplanted lung. This model is feasible and reproducible. However, the short survival time restricts the assessment of the transplanted lung to a maximum period of three hours.
Sánchez
Pablo Gerardo
PG
Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
Martins
Lucas Krieger
LK
Martins
Frederico Krieger
FK
Schimer
Ricardo
R
Cardoso
Paulo Francisco Guerreiro
PF
Andrade
Cristiano Feijó
CF
D000714:Anastomosis, Surgical / Q000295:instrumentation / Q000379:methods; D000818:Animals; D016040:Lung Transplantation / Q000295:instrumentation / Q000379:methods*; D023421:Models, Animal; D011651:Pulmonary Artery / Q000601:surgery*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D016019:Survival Analysis