title: Aberrant expression of histo-blood group A type 3 antigens in vascular endothelial cells in inflammatory sites.
abstract: Histo-blood group ABH antigens are widely distributed in human tissues. The epitopes of ABH antigens are carried by at least four different peripheral core isotypes of internal carbohydrate backbones (type 1-4). Each type of ABH antigen is expressed tissue specifically, and aberrant expression of ABH antigens is often observed during oncogenesis. We immunohistochemically examined the expression of A type 3 antigens in wounded and diseased skin tissues (A and AB blood groups). In uninjured skin, the expression of A type 3 antigens was restricted to the eccrine sweat gland. In addition to the sweat glands, A type 3 antigens were found in vascular endothelial cells of the wound sites. The extent of A type 3 antigens expression related to postinfliction intervals. A significantly higher expression rate of A type 3 antigens in endothelial cells was also observed in diseased skin, suggesting that inflammation might induce A type 3 antigen expression in endothelial cells. Double-color immunofluorescence staining of the specimens showed that von Willebrand factor (vWF) was a core-protein of A type 3 determinants aberrantly expressed in endothelial cells in inflamed tissues, suggesting that aberrant expression of A type 3 antigens is involved in stabilization of vWF in inflammation.
authors.affiliation: Department of Forensic Medicine, Wakayama Medical University, Wakayama, Japan.
authors.forename: Mizuho
authors.initials: M
authors.lastname: Nosaka
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ishida
authors.forename: Aki
authors.initials: A
authors.lastname: Tanaka
authors.forename: Takahito
authors.initials: T
authors.lastname: Hayashi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Miyashita
authors.forename: Chikako
authors.initials: C
authors.lastname: Kaminaka
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Eisenmenger
authors.forename: Fukumi
authors.initials: F
authors.lastname: Furukawa
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kimura
mesh_terms: D000017:ABO Blood-Group System / Q000096:biosynthesis*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D042783:Endothelial Cells / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000378:metabolism; D008875:Middle Aged; D012867:Skin / Q000098:blood supply* / Q000293:injuries; D012871:Skin Diseases / Q000378:metabolism*; D014841:von Willebrand Factor / Q000096:biosynthesis
chemical_list: D000017:ABO Blood-Group System; D014841:von Willebrand Factor