title: Mycobacterium bovis BCG vaccination induces memory CD4+ T cells characterized by effector biomarker expression and anti-mycobacterial activity.
abstract: The effector mechanisms used by CD4+ T cells to control mycobacteria differ between humans and rodent models of TB and should be investigated in additional animal models. In these studies, the bovine model was used to characterize the mycobactericidal CD4+ T cell response induced by vaccination with the attenuated Mycobacterium bovis bacillus Calmette-Guérin (BCG). Antigenic stimulation of peripheral blood CD4+ T cells from BCG-vaccinated cattle enhanced expression of perforin and IFNgamma in cells expressing a CD45RA-CD45RO+CD62L+ cell surface phenotype, enhanced transcription of granulysin, IFNgamma, perforin, IL-4, IL-13, and IL-21, and enhanced anti-mycobacterial activity of CD4+ T cells against BCG-infected macrophages.
authors.affiliation: Department of Pediatrics and Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX 77555, United States.
authors.forename: Janice J
authors.initials: JJ
authors.lastname: Endsley
authors.forename: Alison
authors.initials: A
authors.lastname: Hogg
authors.forename: Lis J
authors.initials: LJ
authors.lastname: Shell
authors.forename: Martin
authors.initials: M
authors.lastname: McAulay
authors.forename: Tracey
authors.initials: T
authors.lastname: Coffey
authors.forename: Chris
authors.initials: C
authors.lastname: Howard
authors.forename: Charles F
authors.initials: CF
authors.lastname: Capinos Scherer
authors.forename: W Ray
authors.initials: WR
authors.lastname: Waters
authors.forename: Brian
authors.initials: B
authors.lastname: Nonnecke
authors.forename: D Mark
authors.initials: DM
authors.lastname: Estes
authors.forename: Bernardo
authors.initials: B
authors.lastname: Villarreal-Ramos
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000378:metabolism; D001500:BCG Vaccine / Q000008:administration & dosage / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D002417:Cattle; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000378:metabolism; D008264:Macrophages / Q000382:microbiology; D009163:Mycobacterium bovis / Q000276:immunology*; D054353:Perforin / Q000378:metabolism; D014376:Tuberculosis / Q000276:immunology / Q000517:prevention & control; D014380:Tuberculosis, Bovine / Q000276:immunology / Q000517:prevention & control*; D014611:Vaccination
chemical_list: D000890:Anti-Infective Agents; D001500:BCG Vaccine; D016207:Cytokines; D054353:Perforin; D007371:Interferon-gamma