A novel nucleotide receptor in Xenopus activates the cAMP second messenger pathway.
We describe a Xenopus P2Y receptor that shares only weak homology with members of the mammalian P2Y family, being most similar to human P2Y(11). When activated by nucleotide analogs, it stimulates both calcium and cAMP mobilization pathways, a feature unique, among mammalian P2Y receptors, to P2Y(11). Activity can be blocked by compounds known to act as antagonists of mammalian P2Y(11). Genomic synteny between Xenopus and mammals suggests that the novel gene is a true ortholog of P2Y(11). Xenopus P2Y(11) is transcribed during embryonic development, beginning at gastrulation, and is enriched in the developing nervous system.
Devader
Christelle
C
Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Drew
Christian M
CM
Geach
Timothy J
TJ
Tabler
Jacqui
J
Townsend-Nicholson
Andrea
A
Dale
Leslie
L
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020013:Calcium Signaling; D000242:Cyclic AMP / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008322:Mammals / Q000235:genetics; D008969:Molecular Sequence Data; D018048:Receptors, Purinergic P2 / Q000235:genetics* / Q000378:metabolism*; D015290:Second Messenger Systems; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics* / Q000378:metabolism*
D017931:DNA Primers; C109734:P2RY11 protein, human; D018048:Receptors, Purinergic P2; D029867:Xenopus Proteins; D000242:Cyclic AMP