title: Reassessment of the phylogenetic relationships of Thiomonas cuprina.
abstract: The published sequence of the 16S rRNA gene of Thiomonas cuprina strain Hö5 (=DSM 5495T) (GenBank accession no. U67162) was found to be erroneous. The 16S rRNA genes from the type strain held by the DSMZ since 1990 (DSM 5495T=NBRC 102145T) and strain Hö5 maintained frozen in the Universität Regensburg for 23 years (=NBRC 102094) were sequenced and found to be identical, but to show no significant similarity to the U67162 sequence. This also casts some doubt on the previously published 5S and 23S rRNA gene sequences (GenBank accession nos U67171 and X75567). The correct 16S rRNA gene sequence showed 99.8% identity to those from Thiomonas delicata NBRC 14566T and 'Thiomonas arsenivorans' DSM 16361. The properties of these three species are re-evaluated, and emended descriptions are provided for the genus Thiomonas and the species Thiomonas cuprina.
authors.affiliation: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
authors.forename: Donovan P
authors.initials: DP
authors.lastname: Kelly
authors.affiliation: NITE Biological Research Center, National Institute of Technology and Evaluation, 2-5-8 Kazasakamatari, Kisarazu-shi, Chiba, 292-0818 Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Uchino
authors.affiliation: Lehrstuhl für Mikrobiologie und Archaeenzentrum, Universität Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Huber
authors.affiliation: Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Amils
authors.affiliation: Department of Microbiology, King's College London, Dental Institute, Floor 28 Guy's Tower, Guy's Campus, London SE1 9RT, UK.
authors.forename: Ann P
authors.initials: AP
authors.lastname: Wood
mesh_terms: D020562:Betaproteobacteria / Q000145:classification* / Q000235:genetics* / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S