[Influence of match-pair of huanglian-rougui on rat intestinal absorption of total huanglian alkali].
OBJECTIVE
To research the principle of match-pair of huanglian-rougui.


METHODS
A rat intestinal model in vitro had been applied to investigate the absorption and transport characteristics of total huanglian alkali across intestinal mucosa, as well as the influence of match-pair of huanglian-rougui on its intestinal absorption. The identification of alkalis in primitive solution, serosal solution and intestinal homogenate were analyzed by TLC.


RESULTS
The transport and uptake characteristic of total alkali in huanglian solution was similar to berberine. The uptake and transport of total alkali across intestinal mucosa was concentration-and time-dependent. Transport directions had strong effects on the transport of total alkali. The amount of total alkali transport from serosal side to mucosal side was much more than that from mucosal side to serosal side. After matching with rougui as some ration, the intestinal absorption of total alkali increased significantly comparing with huanglian alone, and there was an optimal ration among match-pair of huanglian-rougui.


CONCLUSIONS
Chemical reaction in vitro and intestinal absorption were the key factors of match-pair of huanglian-rougui.
Zhang
Yu-jie
YJ
School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China. zhy55227@126.com
Yang
Jie
J
Zou
Xiao-cui
XC
Liu
Li-mei
LM
Sun
Wan-jing
WJ
D000470:Alkaloids / Q000302:isolation & purification / Q000493:pharmacokinetics*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D027424:Cinnamomum / Q000737:chemistry; D039323:Coptis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D016555:Matched-Pair Analysis; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
D000470:Alkaloids; D004365:Drugs, Chinese Herbal