title: Altered inflammatory responses in TLR5-deficient mice infected with Legionella pneumophila.
abstract: Legionella pneumophila (Lp), an important cause of morbidity and mortality from pneumonia, infects alveolar macrophages (AMs) and is recognized by several TLRs as well as Birc1e (NAIP5) and IL-1 converting enzyme-protease activating factor. We examined the role of TLR5 during the murine response to aerosolized Lp infection. At 4 h after infection, Tlr5(-/-) mice had lower numbers of polymorphonuclear neutrophils (PMNs) in their broncho-alveolar lavage fluid in comparison to wild-type (WT) mice. At 24 and 72 h, the PMN recruitment was similar. WT mice infected with a flagellin-deficient strain (LpFlaA-) also showed an impaired early PMN response at 4 h compared with those infected with the WT strain. There was no consistent difference in bacterial counts at any of the time points when comparing the Tlr5(-/-) and WT mice. However, at 6 days after infection, the Tlr5(-/-) mice had increased leukocytic infiltrates in the alveolar and peribronchial interstitial spaces that were consistent with organizing pneumonia. We also examined the role of TLR5 during macrophage infection. In contrast to bone marrow-derived macrophages, AMs secreted TNF-alpha after stimulation with purified flagellin. In addition, WT, but not Tlr5(-/-), AMs produced TNF-alpha after stimulation with Lp. Live LpFlaA- did not induce TNF-alpha secretion in AM. These results suggested that AMs recognize Lp flagellin and that a majority of the Lp-induced TNF-alpha response is TLR5-mediated. Thus, TLR5 mediates recognition of Lp in AMs and performs a distinct role during the in vivo pulmonary immune response through regulation of early PMN recruitment and subsequent later development of pneumonia.
authors.affiliation: Department of Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA. thawn@u.washington.edu
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Hawn
authors.forename: William R
authors.initials: WR
authors.lastname: Berrington
authors.forename: Ian A
authors.initials: IA
authors.lastname: Smith
authors.forename: Satoshi
authors.initials: S
authors.lastname: Uematsu
authors.forename: Shizuo
authors.initials: S
authors.lastname: Akira
authors.forename: Alan
authors.initials: A
authors.lastname: Aderem
authors.forename: Kelly D
authors.initials: KD
authors.lastname: Smith
authors.forename: Shawn J
authors.initials: SJ
authors.lastname: Skerrett
mesh_terms: D000818:Animals; D001980:Bronchi / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D005408:Flagellin / Q000235:genetics / Q000276:immunology*; D007249:Inflammation / Q000235:genetics / Q000276:immunology / Q000473:pathology; D016952:Legionella pneumophila / Q000235:genetics / Q000276:immunology*; D007877:Legionnaires' Disease / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D051616:Neuronal Apoptosis-Inhibitory Protein / Q000235:genetics / Q000276:immunology; D018410:Pneumonia, Bacterial / Q000235:genetics / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D011650:Pulmonary Alveoli / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D013997:Time Factors; D051198:Toll-Like Receptor 5 / Q000235:genetics / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: C496806:Naip5 protein, mouse; D051616:Neuronal Apoptosis-Inhibitory Protein; D051198:Toll-Like Receptor 5; D014409:Tumor Necrosis Factor-alpha; D005408:Flagellin