Bodipy-VAD-Fmk, a useful tool to study yeast peptide N-glycanase activity.
In this paper the development of a fluorescent activity based probe, Bodipy-VAD-Fmk, for visualization of yeast peptide N-glycanase is described. The activity based probe is used to assess the efficacy of known and new chitobiose-based electrophilic traps to bind yeast peptide N-glycanase.
Witte
Martin D
MD
Bioorganic Synthesis, Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
Descals
Carlos V
CV
de Lavoir
Sebastiaan V P
SV
Florea
Bogdan I
BI
van der Marel
Gijsbert A
GA
Overkleeft
Herman S
HS
D000590:Amino Acid Chloromethyl Ketones / Q000737:chemistry / Q000378:metabolism*; D005453:Fluorescence; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D000590:Amino Acid Chloromethyl Ketones; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase