title: Molecular cytogenetics of Salminus fish (Characiformes) based on 5S and 18S rRNA genes hybridization, fluorochrome staining and C-banding.
abstract: Karyotype, mapping of nucleolar and 5S rRNA genes and distribution of constitutive heterochromatin supposedly AT-rich were characterized on two isolate populations of Salminus brasiliensis, the biggest characid fish, and three population of Salminus hilarii. The diploid number 2n=50 and the karyotype formulae (10M+20SM+20ST/A) were the same to Salminus species studied. The position of 18S rDNA cluster identified by FISH coincide with chromomycin A3 labeling (CMA+) in the long arm telomeric portion of sixth pair. Subtle differences for the disposal of the 5S rRNA gene in the chromosome of the Salminus are presented. The distribution of the constitutive heterochromatins and DA/DAPI+ bands are described.
authors.affiliation: Departamento de Ciências Biológicas e da Saúde, Instituto Universitário do Araguaia, Universidade Federal de Mato Grosso, Rodovia MT 100, km 3.5, CEP 78698-000, Pontal do Araguaia MT, Brazil. issakar@pq.cnpq.br
authors.forename: Issakar Lima
authors.initials: IL
authors.lastname: Souza
authors.forename: Ludier Kesser
authors.initials: LK
authors.lastname: Santos-Silva
authors.forename: Paulo César
authors.initials: PC
authors.lastname: Venere
authors.forename: Orlando
authors.initials: O
authors.lastname: Moreira-Filho
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D002875:Chromosomes / Q000235:genetics; D020732:Cytogenetic Analysis; D005399:Fishes / Q000145:classification / Q000235:genetics*; D005456:Fluorescent Dyes / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D009693:Nucleic Acid Hybridization; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes; D012337:RNA, Ribosomal, 18S; D012341:RNA, Ribosomal, 5S