title: Separation and identification of plasma short-chain acylcarnitine isomers by HPLC/MS/MS for the differential diagnosis of fatty acid oxidation defects and organic acidemias.
abstract: This paper reports a new, high-performance liquid chromatography/tandem mass spectrometry method for the separation and identification of human plasma short-chain acylcarnitine isomers. This simple, rapid procedure involves the use of a single sample previously shown to contain elevated acylcarnitine concentrations by flow injection analysis, and can separate two C4, three C5, two C5:1 and four C5-OH acylcarnitine isomers, thus permitting the differential diagnosis of certain fatty acid oxidation defects and organic acidemias.
authors.affiliation: Centro de Diagnóstico de Enfermedades Moleculares, Departamento de Biología Molecular, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors.forename: Isaac
authors.initials: I
authors.lastname: Ferrer
authors.forename: Pedro
authors.initials: P
authors.lastname: Ruiz-Sala
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Vicente
authors.forename: Begoña
authors.initials: B
authors.lastname: Merinero
authors.forename: Celia
authors.initials: C
authors.lastname: Pérez-Cerdá
authors.forename: Magdalena
authors.initials: M
authors.lastname: Ugarte
mesh_terms: D002331:Carnitine / Q000031:analogs & derivatives* / Q000097:blood / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D007536:Isomerism; D008297:Male; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C116917:acylcarnitine; D002331:Carnitine