Bovine endothelial cells interact with fully-luteinized, but not luteinizing, granulosa cells in the mRNA expression of endothelin-1 system in response to prostaglandin F(2alpha).
The corpus luteum (CL) undergoes regression by prostaglandin (PG)F(2alpha) from uterus and endothelin-1 (ET-1) plays an important role during luteolysis as a local mediator of PGF(2alpha) in the cow. Endothelial cells (EC) and luteal cells are main cell types making up the CL and their interactions are vital for CL function. We aimed to examine the relevance of interactions between EC and luteal cells on stimulation of genes which involved ET-1 synthesis by PGF(2alpha). We further focused the impact of maturity of luteal cells on the stimulation of the genes. To make a microenvironment which resembles the CL, we used bovine aortic endothelial cells (BAEC) and luteinizing or fully-luteinized granulosa cells (GC) and evaluated the effect of PGF(2alpha) on the expression for mRNA of ET-1 system by using real-time RT-PCR. PGF(2alpha) stimulated the expression of preproET-1 and endothelin converting enzyme-1 mRNA only in the co-cultures of BAEC with fully-luteinized GC, but not with luteinizing GC. The data suggest that interactions between BAEC and fully-luteinized GC enhance the capability of BAEC to produce ET-1 in response to PGF(2alpha). This mechanism may contribute to the local induction of luteolytic action of PGF(2alpha) which is dependent on the age/maturation of the CL.
Shirasuna
K
K
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
Watanabe
S
S
Yamamoto
D
D
Hayashi
M
M
Nagai
K
K
Miyamoto
A
A
D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism; D002417:Cattle; D018920:Coculture Techniques / Q000662:veterinary; D003338:Corpus Luteum / Q000502:physiology; D015237:Dinoprost / Q000494:pharmacology*; D042783:Endothelial Cells / Q000502:physiology*; D019332:Endothelin-1 / Q000235:genetics / Q000378:metabolism*; D000072396:Endothelin-Converting Enzymes; D005260:Female; D015870:Gene Expression; D006107:Granulosa Cells / Q000187:drug effects / Q000502:physiology; D008184:Luteal Cells / Q000187:drug effects / Q000502:physiology*; D003341:Luteolysis / Q000502:physiology; D008666:Metalloendopeptidases / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D019332:Endothelin-1; D012333:RNA, Messenger; D015237:Dinoprost; D016282:Aspartic Acid Endopeptidases; D008666:Metalloendopeptidases; D000072396:Endothelin-Converting Enzymes