Transgenic overexpression of ADAM12 suppresses muscle regeneration and aggravates dystrophy in aged mdx mice.
Muscular dystrophies are characterized by insufficient restoration and gradual replacement of the skeletal muscle by fat and connective tissue. ADAM12 has previously been shown to alleviate the pathology of young dystrophin-deficient mdx mice, a model for Duchenne muscular dystrophy. The observed effect of ADAM12 was suggested to be mediated via a membrane-stabilizing up-regulation of utrophin, alpha7B integrin, and dystroglycans. Ectopic ADAM12 expression in normal mouse skeletal muscle also improved regeneration after freeze injury, presumably by the same mechanism. Hence, it was suggested that ADAM12 could be a candidate for nonreplacement gene therapy of Duchenne muscular dystrophy. We therefore evaluated the long-term effect of ADAM12 overexpression in muscle. Surprisingly, we observed loss of skeletal muscle and accelerated fibrosis and adipogenesis in 1-year-old mdx mice transgenically overexpressing ADAM12 (ADAM12(+)/mdx mice), even though their utrophin levels were mildly elevated compared with age-matched controls. Thus, membrane stabilization was not sufficient to provide protection during prolonged disease. Consequently, we reinvestigated skeletal muscle regeneration in ADAM12 transgenic mice (ADAM12(+)) after a knife cut lesion and observed that the regeneration process was significantly impaired. ADAM12 seemed to inhibit the satellite cell response and delay myoblast differentiation. These results discourage long-term therapeutic use of ADAM12. They also point to impaired regeneration as a possible factor in development of muscular dystrophy.
Jørgensen
Louise Helskov
LH
Department of Clinical Pathology, Odense University Hospital, Odense C, Denmark.
Jensen
Charlotte Harken
CH
Wewer
Ulla M
UM
Schrøder
Henrik Daa
HD
D051722:ADAM Proteins / Q000096:biosynthesis* / Q000235:genetics; D000072199:ADAM12 Protein; D000375:Aging / Q000473:pathology*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation; D016189:Dystrophin / Q000235:genetics; D005355:Fibrosis; D039001:Integrin alpha Chains / Q000378:metabolism; D051379:Mice; D018101:Mice, Inbred mdx; D008822:Mice, Transgenic; D009137:Muscular Dystrophy, Animal / Q000378:metabolism* / Q000473:pathology; D020388:Muscular Dystrophy, Duchenne / Q000378:metabolism* / Q000473:pathology; D032446:Myoblasts / Q000502:physiology; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000502:physiology; D049411:Utrophin / Q000378:metabolism
D015703:Antigens, CD; D016189:Dystrophin; D039001:Integrin alpha Chains; D049411:Utrophin; C085725:integrin alpha7; D051722:ADAM Proteins; D000072199:ADAM12 Protein; C096115:Adam12 protein, mouse