Assays of T-cell contact dependent monocyte-macrophage functions.
The production of cytokines and other inflammatory products in chronic/sterile inflammatory disorders such as rheumatoid arthritis might be induced in monocytes/macrophages by direct cellular contact with stimulated T-cells. Studies of cell-cell interactions are usually complicated by the simultaneous presence of at least two viable cell types. To obviate this problem, we developed strategies allowing only interactions between stimulated T-cell surface molecules and the monocytes/macrophages, and any products secreted into the medium or mRNA are necessarily of monocytic origin. This chapter aims at reviewing and describing the latter methods and strategies.
Burger
Danielle
D
Clinical Immunology Unit, University Hospital, Geneva, Switzerland.
Dayer
Jean-Michel
JM
D002469:Cell Separation / Q000379:methods*; D016207:Cytokines / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D008264:Macrophages / Q000166:cytology / Q000276:immunology*; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D016207:Cytokines; D012333:RNA, Messenger