Bone regeneration by BMP-2 enhanced adipose stem cells loading on alginate gel.
Adipose stem cells (ASCs) have the potential to differentiate into a variety of cell lineages both in vitro and in vivo. In this study, ASCs were harvested from normal Sprague-Dawley (SD) rats and transfected by BMP-2 gene before they were loaded on alginate. The ability of bone regeneration was determined in rat critical-size cranial defects. An 8-mm diameter defect was created in the calvarias of 36 rats; these rats were divided into three groups. In experimental group, the defects were filled with alginate gel combined with BMP-2 transfected ASCs; in negative control group, the defects were filled with alginate gel mixed with normal ASCs; in blank controls, the defects were filled with cell-free alginate gel. Four rats of each group were killed and the cranial defect sites were observed at 4, 8 and 16 weeks after surgery. There was complete repair of cranial defects in experimental group using the alginate gel loading BMP-2 transfected ASC, but only partial repair in negative controls and in the blank control. The engineering approach combining BMP-2 enhanced ASCs with alginate gel can therefore stimulate bone regeneration and repair for the large size bone defects.
Lin
Yunfeng
Y
Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Tang
Wei
W
Wu
Ling
L
Jing
Wei
W
Li
Xiaoyu
X
Wu
Yao
Y
Liu
Lei
L
Long
Jie
J
Tian
Weidong
W
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation*; D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis / Q000235:genetics*; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D005782:Gels; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012886:Skull / Q000293:injuries; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics*
D000464:Alginates; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D005782:Gels; D016212:Transforming Growth Factor beta