title: Inhibitory effect of eupatilin and jaceosidin isolated from Artemisia princeps in IgE-induced hypersensitivity.
abstract: To understand the antiallergic effect of Artemisia princeps (AP), which has been found to show inhibitory activity against degranulation and a passive cutaneous anaphylaxis (PCA) reaction, eupatilin and jaceosidin, as the active components, were isolated by degranulation-inhibitory activity-guided fractionation, with their antiallergic activity investigated. These isolated components potently inhibited the release of beta-hexosaminidase from RBL-2H3 cells induced by the IgE-antigen complex, with IC(50) values of 3.4 and 4.5muM, respectively. Eupatilin and jaceosidin potently inhibited the PCA reaction and scratching behaviors induced by IgE- antigen complex and compound 48/80, respectively. Orally administered jaceosidin more potently inhibited the PCA reaction than that of eupatilin, although the PCA reaction-inhibitory activity of intraperitoneally administered jaceosidin was nearly the same as that of eupatilin. Eupatilin and jaceosidin inhibited the gene expressions of TNF-alpha and IL-4 in RBL-2H3 cells stimulated by IgE-antigen complex. Eupatilin and jaceosidin inhibited the activation of NF-kB. Based on these findings, eupatilin and jaceosidin may be useful for protection from the PCA and itching reactions, which are IgE-mediated representative skin allergic diseases.
authors.affiliation: College of Pharmacy, Kyung-Hee University, 1, Hoegi, Dongdaemun-ku, Seoul 130-701, South Korea.
authors.forename: Seung Hoon
authors.initials: SH
authors.lastname: Lee
authors.forename: Eun-Ah
authors.initials: EA
authors.lastname: Bae
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Park
authors.forename: Yong-Wook
authors.initials: YW
authors.lastname: Shin
authors.forename: Nam-In
authors.initials: NI
authors.lastname: Baek
authors.forename: Eun-Joo
authors.initials: EJ
authors.lastname: Han
authors.forename: Hae-Gon
authors.initials: HG
authors.lastname: Chung
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
mesh_terms: D000818:Animals; D019306:Artemisia / Q000737:chemistry*; D016207:Cytokines / Q000096:biosynthesis; D005419:Flavonoids / Q000494:pharmacology*; D006967:Hypersensitivity / Q000188:drug therapy*; D007073:Immunoglobulin E / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000378:metabolism; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D016207:Cytokines; D005419:Flavonoids; D016328:NF-kappa B; C477508:jaceosidin; D007073:Immunoglobulin E; C045325:eupatilin; D001619:beta-N-Acetylhexosaminidases