title: Thermometric MIP sensor for fructosyl valine.
abstract: Interactions of molecularly imprinted polymers containing phenyl boronic acid residues with fructosyl valine, fructose and pinacol, respectively are analysed in aqueous solution (pH 11.4) by using a flow calorimeter. The reversible formation of (two) cyclic boronic acid diesters per fructosyl molecule generates a 40-fold higher exothermic signal as compared to the control polymer. Whereas binding of pinacol to either the MIP or the control polymer generates a very small endothermic signal reflecting a negligible contribution of the esterification to the overall process. An "apparent imprinting factor" of 41 is found which exceeds the respective value of batch binding procedures by a factor of 30. Furthermore, the MIP sensor was used to characterise the crossreactivity. The influence of shape selective molecular recognition is discussed.
authors.affiliation: Analytical Biochemistry, Potsdam University, Potsdam-Golm, Germany.
authors.forename: Rajagopal
authors.initials: R
authors.lastname: Rajkumar
authors.forename: Martin
authors.initials: M
authors.lastname: Katterle
authors.forename: Axel
authors.initials: A
authors.lastname: Warsinke
authors.forename: Helmuth
authors.initials: H
authors.lastname: Möhwald
authors.forename: Frieder W
authors.initials: FW
authors.lastname: Scheller
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002151:Calorimetry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties; D013817:Thermography / Q000295:instrumentation*; D014633:Valine / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D011108:Polymers; C045420:fructosylvaline; D014633:Valine