Detection of human papillomavirus type 31-neutralizing antibodies from naturally infected patients by an assay based on intracellular assembly of luciferase-expressing pseudovirions.
The aim of this study was to develop a highly sensitive human papillomavirus type 31 (HPV31) neutralization assay based on the production of pseudovirions carrying luciferase. Neutralizing antibodies against HPV31 were investigated in a set of HPV31 monoclonal antibodies and in women with evidence of HPV31 infection. Neutralizing antibodies were detected in 78% of subjects with a positive enzyme-linked immunosorbent assay.
Fleury
Maxime J J
MJ
INSERM U618, Tours, France.
Touzé
Antoine
A
de Sanjosé
Silvia
S
Bosch
F Xavier
FX
Klaustermeiyer
Joellen
J
Coursaget
Pierre
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007313:Insecta; D008156:Luciferases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D009500:Neutralization Tests; D027383:Papillomaviridae / Q000276:immunology* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D014771:Virion / Q000201:enzymology* / Q000235:genetics; D019065:Virus Assembly / Q000502:physiology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D008156:Luciferases