[Modulation of Jianjining Recipe on differential protein expression in rats with experimental autoimmune myasthenia gravis].
OBJECTIVE
To study the modulation of Jianjining Recipe (JJNR), a traditional Chinese compound herbal medicine for invigorating spleen and kidney on differential protein expression in spleen of rats with experimental autoimmune myasthenia gravis (EAMG).


METHODS
EAMG rats were randomly divided into four groups: untreated group, JJNR-treated group, Qiangji Jianli capsule (QJJLC, a traditional Chinese compound herbal medicine)-treated group and prednisolone acetate (PA)-treated group. After therapeutic intervention with the above drugs for four consecutive weeks, the level of differential protein expression was analyzed by two-dimensional electrophoresis and matrix assisted laser desorption/ionization time-of-flight mass spectrometry.


RESULTS
Twelve differential proteins were identified by comparing EAMG rats and normal rats. The levels of allograft inflammatory factor-1, peroxiredoxin I and actin-related protein 2/3 complex subunit 5 were significantly regulated by JJNR (P<0.01). These proteins were closely associated with immune response and cell movement.


CONCLUSIONS
The results suggest that there are differential protein expressions between EAMG rats and normal rats. Furthermore, as a Chinese medicine prescription with effect of invigorating spleen and kidney, JJNR can effectively regulate the levels of some EAMG-related protein expression.
Liu
Ping
P
Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. liuping23@sina.com
Ding
Xu-feng
XF
Zhang
Yi-yi
YY
Qiao
Jian
J
D051376:Actin-Related Protein 2-3 Complex / Q000032:analysis; D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D008840:Microfilament Proteins; D020720:Myasthenia Gravis, Autoimmune, Experimental / Q000188:drug therapy* / Q000378:metabolism; D054464:Peroxiredoxins / Q000032:analysis*; D011897:Random Allocation; D051381:Rats; D011917:Rats, Inbred Lew
D051376:Actin-Related Protein 2-3 Complex; C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D004365:Drugs, Chinese Herbal; D008840:Microfilament Proteins; D054464:Peroxiredoxins