title: Production and characterization of a monoclonal antibody against GPR40 (FFAR1; free fatty acid receptor 1).
abstract: GPR40 is G protein-coupled receptor whose endogenous ligands have recently been identified as free fatty acids (FFAs), and it has been implicated to play an important role in FFA-mediated enhancement of glucose-stimulated insulin release. We have developed a monoclonal antibody against the extracellular domain of GPR40. Specificity of the antibody was demonstrated by immunoprecipitation and cell surface staining using GPR40-transfected cells. GPR40 immunoreactivity was highly abundant in mouse pancreatic beta-cells and splenocytes, THP-1 cells, and human peripheral blood mononuclear cells. The anti-GPR40 monoclonal antibody should prove valuable for further studying the function of this nutrient sensing receptor.
authors.affiliation: Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-shimo-adachi-cho, Sakyo-ku, Kyoto 606-8501, Japan. akira_h@pharm.kyoto-u.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Hirasawa
authors.forename: Chisato
authors.initials: C
authors.lastname: Itsubo
authors.forename: Keiko
authors.initials: K
authors.lastname: Sadakane
authors.forename: Takafumi
authors.initials: T
authors.lastname: Hara
authors.forename: Shingo
authors.initials: S
authors.lastname: Shinagawa
authors.forename: Hanako
authors.initials: H
authors.lastname: Koga
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nose
authors.forename: Taka-aki
authors.initials: TA
authors.lastname: Koshimizu
authors.forename: Gozoh
authors.initials: G
authors.lastname: Tsujimoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology*; D000918:Antibody Specificity; D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000032:analysis / Q000276:immunology*; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; C473034:FFAR1 protein, human; C473036:Ffar1 protein, mouse; D010446:Peptide Fragments; D043562:Receptors, G-Protein-Coupled