[Effect of artemisinin on ischemia/reperfusion injury of isolated rat myocardium].
OBJECTIVE
To observe the effect of artemisinin on the ischemia/reperfusion injury of the iisolated rat myocardium and to preliminarily study the possible mechanism.


METHODS
Fifty Wistar rats were randomly divided into 5 groups: a control group, an ischemia/reperfusion (I/R) group, and 3 artemisinin (AS) groups (10, 100, 1000 micromol x L(-1)), 10 rats in each group. Ischemia/reperfusion injury of the isolated rat myocardium was induced by a Langendorff system. The electrocardiogram, the cardiac functional parameters, coronary flow, and the activities of LDH (lactate dehydrogenase), CPK (creatine phosphokinase), SOD (superoxide dis-mutase) and the level of malondiadehyde (MDA) in myocardial tissue, and the myocardial ultrastructures were investigated.


RESULTS
AS (10,100 micromol x L(-1)) could significantly improve the index of the myocardial function (+/- dp/dt(max), LVSP) after the ischemia/reperfusion, increase the coronary flow, decrease the leakage of LDH and CPK, and increase the SOD activity and decrease the MDA level in cardiac tissues, and alleviate the myocardial ultrastructure injury. But, AS (1000 micromo x L(-1)) did not have the above effects.


CONCLUSIONS
AS (10, 100 micromol x L(-1)) alleviate the myocardial ischemia/reperfusion injury in rats. The mechanism may be related to its functions of antioxidation and scavenging free radicals.
Sun
Li-hong
LH
Department of Pathophysiology, Harbin Medical University, Harbin 150086, China.
Li
Hong-zhu
HZ
Han
Li-ping
LP
Jiang
Chun-ming
CM
Zhao
Ya-jun
YJ
Gao
Xiu-xiang
XX
Tian
Ye
Y
Xu
Chang-qing
CQ
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D019306:Artemisia / Q000737:chemistry; D037621:Artemisinins / Q000302:isolation & purification / Q000494:pharmacology*; D003326:Coronary Circulation / Q000187:drug effects; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006321:Heart / Q000503:physiopathology; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D009206:Myocardium / Q000473:pathology*; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D037621:Artemisinins; D016166:Free Radical Scavengers; C031327:artemisinin