Drosophila gypsy insulator and yellow enhancers regulate activity of yellow promoter through the same regulatory element.
There is ample evidence that the enhancers of a promoterless yellow locus in one homologous chromosome can activate the yellow promoter in the other chromosome where the enhancers are inactive or deleted, which is indicative of a high specificity of the enhancer-promoter interaction in yellow. In this paper, we have found that the yellow sequence from -100 to -69 is essential for stimulation of the heterologous eve (TATA-containing) and white (TATA-less) promoters by the yellow enhancers from a distance. However, the presence of this sequence is not required when the yellow enhancers are directly fused to the heterologous promoters or are activated by the yeast GAL4 activator. Unexpectedly, the same promoter proximal region defines previously described promoter-specific, long-distance repression of the yellow promoter by the gypsy insulator on the mod(mdg4) ( u1 ) background. These finding suggest that proteins bound to the -100 to -69 sequence are essential for communication between the yellow promoter and upstream regulatory elements.
Melnikova
Larisa
L
Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., Moscow 119334, Russia.
Kostuchenko
Margarita
M
Silicheva
Margarita
M
Georgiev
Pavel
P
D000818:Animals; D003433:Crosses, Genetic; D004331:Drosophila melanogaster; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D019476:Insect Proteins / Q000378:metabolism; D008957:Models, Genetic; D008958:Models, Molecular; D009154:Mutation; D010858:Pigmentation; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D018626:Retroelements; D014157:Transcription Factors / Q000235:genetics*; D019076:Transgenes
D019476:Insect Proteins; D011994:Recombinant Proteins; D018626:Retroelements; D014157:Transcription Factors; C110987:mdg4 protein (gypsy)