RANTES gene promoter polymorphisms are associated with bronchial hyperresponsiveness in Korean children with asthma.
Regulated upon activation in normal T cells, expressed, and secreted (RANTES) protein is abundantly expressed during atopic asthma, suggesting that it is an important mediator of this disease. The aim of this study was to evaluate the possible role of RANTES promoter polymorphisms in children with asthma. We genotyped 271 children with atopic asthma, 55 children with nonatopic asthma, and 253 control children for allelic determinants at two polymorphic sites in the promoter region at positions -403G>A and -28C>G by restriction fragment length polymorphism methods. There was no significant difference in genotype and allele frequencies of the RANTES -403G>A and -28C>G polymorphisms when the atopic asthma, nonatopic asthma, and control groups were compared. However, atopic asthmatic patients who were homozygous GG for the RANTES -28C>G tended to have lower PC20 methacholine than those carrying the wild genotype. In addition, a significantly lower PC20 was demonstrated for the homozygous haplotype -403A/-28G in asthmatic children. The polymorphisms within the RANTES promoter may have a disease-modifying effect in Korean children with asthma.
Sohn
Myung Hyun
MH
Department of Pediatrics and Institute of Allergy, Yonsei University College of Medicine, Brain Korea 21 Project for Medical Sciences, Seoul, Korea.
Kim
Seung-Hyun
SH
Kim
Kyung-Won
KW
Jee
Hye Mi
HM
Park
Hae-Sim
HS
Kim
Kyu-Earn
KE
D000293:Adolescent; D001249:Asthma / Q000175:diagnosis / Q000235:genetics* / Q000503:physiopathology; D016535:Bronchial Hyperreactivity / Q000175:diagnosis / Q000235:genetics* / Q000503:physiopathology; D001985:Bronchial Provocation Tests; D016085:Bronchoconstrictor Agents; D016022:Case-Control Studies; D018946:Chemokine CCL5 / Q000235:genetics*; D002648:Child; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006720:Homozygote; D006801:Humans; D007723:Korea; D008297:Male; D016210:Methacholine Chloride; D010641:Phenotype; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012307:Risk Factors
D016085:Bronchoconstrictor Agents; C483024:CCL5 protein, human; D018946:Chemokine CCL5; D016210:Methacholine Chloride