title: Influence of oxygen tension on CD133 phenotype in human glioma cell cultures.
abstract: Under standard culture conditions, tumor cells are exposed to 20% O(2), whereas the mean tumor oxygen levels within the tumor are much lower. We demonstrate, using low-passaged human tumor cell cultures established from glioma, that a reduction in the oxygen level in these cell cultures dramatically increases the percentage of CD133 expressing cells.
authors.affiliation: INSERM, U836, Université Joseph Fourier, CHU, Grenoble, F-38043, France.
authors.forename: Nadine
authors.initials: N
authors.lastname: Platet
authors.forename: Shi Yong
authors.initials: SY
authors.lastname: Liu
authors.forename: Michèle El
authors.initials: ME
authors.lastname: Atifi
authors.forename: Lisa
authors.initials: L
authors.lastname: Oliver
authors.forename: François M
authors.initials: FM
authors.lastname: Vallette
authors.forename: François
authors.initials: F
authors.lastname: Berger
authors.forename: Didier
authors.initials: D
authors.lastname: Wion
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005910:Glioma / Q000378:metabolism / Q000473:pathology; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D010100:Oxygen / Q000494:pharmacology*; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D010100:Oxygen