Helicobacter pylori isolated from a patient with Ménétrier's disease increases hepatocyte growth factor mRNA expression in gastric fibroblasts: comparison with Helicobacter pylori isolated from other gastric diseases.
Ménétrier's disease has been reported to be associated with Helicobacter pylori infection. The aim of this study was to investigate the genetic characteristics of various virulence factors and cytokine expression profiles in Helicobacter pylori isolated from patients with Ménétrier's disease. The genotyping of virulence factors was accomplished by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Induction of various cytokines in MKN45 cells or gastric fibroblasts by Helicobacter pylori stimulus was measured by real-time reverse transcription-PCR. We found that the Helicobacter pylori strain isolated from a patient with Ménétrier's disease was different from other strains in the MseI-RFLP pattern of the ureC gene. Helicobacter pylori isolated from a patient with Ménétrier's disease showed the highest hepatocyte growth factor (HGF) and TNF-alpha mRNA expressions from gastric fibroblasts, and the highest TNF-alpha expression from MKN45 cells. The results in this study suggest that the difference in cytokine production, depending on the difference in bacteria components, plays an important role in the development of Ménétrier's disease.
Ishikawa
Takeshi
T
Department of Gastroenterology, Matsushita Memorial Hospital, 5-55 Sotojima-cho, Moriguchi City, Osaka 570-8540, Japan. ishikawa.takeshi002@jp.panasonic.com
Ando
Takashi
T
Obayashi
Hiroshi
H
Nakabe
Nami
N
Okita
Mika
M
Isozaki
Yutaka
Y
Nagao
Yasuyuki
Y
Oyamada
Hirokazu
H
Nakajima
Yoshihiro
Y
Kato
Haruki
H
Kokura
Satoshi
S
Naito
Yuji
Y
Yoshida
Norimasa
N
Yoshikawa
Toshikazu
T
D016207:Cytokines / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D005758:Gastritis, Hypertrophic / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D005838:Genotype; D016481:Helicobacter Infections / Q000150:complications* / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013272:Stomach Diseases / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014774:Virulence
D016207:Cytokines; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D017228:Hepatocyte Growth Factor