Glyoxal fixation: how it works and why it only occasionally needs antigen retrieval.
Over the past 13 years, glyoxal has become the leading alternative to formaldehyde as a histological fixative because of its low inhalation risk, faster reaction rate and selective control over crosslinking. The latter attribute is especially important, because most of the difficulties relating to use of formaldehyde-fixed specimens for immunohistochemistry stem from its aggressive crosslinking behavior. With suitable catalysts or other reaction accelerators, glyoxal forms 2-carbon adducts with nearly all end groups in proteins and carbohydrates, leaving most of them unimpaired for subsequent immunohistochemical demonstration. Only arginine is seriously impaired by the formation of imidazoles, which is the basis for the well known arginine blockade method using glyoxal. A special glyoxal-specific antigen retrieval method using high pH and high temperature effectively reverses the blockade and restores immunoreactivity. Other methods for antigen retrieval are rarely beneficial and in most cases damage the specimen. Special stains work well, except silver methods for Helicobacter pylori. Routine hematoxylin and eosin preparations exhibit clarity and cellular detail rarely seen with formaldehyde.
Dapson
R W
RW
Dapson & Dapson, LLC, Richland, MI, USA. dick@dapsons.com
D000939:Epitopes; D005404:Fixatives / Q000737:chemistry*; D006037:Glyoxal / Q000737:chemistry*; D006801:Humans; D007093:Imidazoles / Q000737:chemistry; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008872:Microwaves; D011487:Protein Conformation; D016707:Tissue Fixation
D000939:Epitopes; D005404:Fixatives; D007093:Imidazoles; D006037:Glyoxal; C029899:imidazole