title: Molecular characterization of BjussuSP-I, a new thrombin-like enzyme with procoagulant and kallikrein-like activity isolated from Bothrops jararacussu snake venom.
abstract: A thrombin-like enzyme, named BjussuSP-I, isolated from Bothrops jararacussu snake venom, is an acidic single-chain glycoprotein with M(r)=61,000, pI approximately 3.8 and 6% sugar. BjussuSP-I shows high proteolytic activity upon synthetic substrates, such as S-2238 and S-2288. It also shows procoagulant and kallikrein-like activity, but is unable to act on platelets and plasmin. These activities are inhibited by specific inhibitors of this class of enzymes. The complete cDNA sequence of BjussuSP-I with 696bp encodes open reading frames of 232 amino acid residues, which conserve the common domains of thrombin-like serine proteases. BjussuSP-I shows a high structural homology with other thrombin-like enzymes from snake venoms where common amino acid residues are identified as those corresponding to the catalytic site and subsites S1, S2 and S3 already reported. In this study, we also demonstrated the importance of N-linked glycans to improve thrombin-like activity of BjussuSP-I toxin.
authors.affiliation: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto da Universidade de São Paulo, FCFRP-USP, Ribeirão Preto, SP, Brazil.
authors.forename: Carolina D
authors.initials: CD
authors.lastname: Sant'Ana
authors.forename: Carolina P
authors.initials: CP
authors.lastname: Bernardes
authors.forename: Luiz Fernando M
authors.initials: LF
authors.lastname: Izidoro
authors.forename: Maurício V
authors.initials: MV
authors.lastname: Mazzi
authors.forename: Sandro G
authors.initials: SG
authors.lastname: Soares
authors.forename: André L
authors.initials: AL
authors.lastname: Fuly
authors.forename: Russolina B
authors.initials: RB
authors.lastname: Zingali
authors.forename: Angelo J
authors.initials: AJ
authors.lastname: Magro
authors.forename: Antonio S K
authors.initials: AS
authors.lastname: Braz
authors.forename: Marcos R M
authors.initials: MR
authors.lastname: Fontes
authors.forename: Rodrigo G
authors.initials: RG
authors.lastname: Stábeli
authors.forename: Suely V
authors.initials: SV
authors.lastname: Sampaio
authors.forename: Andreimar M
authors.initials: AM
authors.lastname: Soares
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001779:Blood Coagulation Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D017837:Bothrops; D003001:Cloning, Molecular; D003435:Crotalid Venoms / Q000201:enzymology*; D018076:DNA, Complementary / Q000378:metabolism; D007610:Kallikreins / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D013917:Thrombin / Q000737:chemistry; D013918:Thrombin Time
chemical_list: D001779:Blood Coagulation Factors; D003435:Crotalid Venoms; D018076:DNA, Complementary; D007610:Kallikreins; D012697:Serine Endopeptidases; D013917:Thrombin