title: Comparison of ADVIA Centaur and Pharmacia UniCAP tests in the diagnosis of food allergy in children with atopic dermatitis.
abstract: In a study comprising 63 children diagnosed with atopic dermatitis, the results of the ADVIA Centaur system was compared with the results obtained with the Pharmacia UniCAP100 system, which has been widely considered as a reference method for seric specific IgE (sIgE) measurements. The individual immunization against the most common food allergens [egg (f1), cow milk (f2), cod (f3), wheat (f4), peanut (f13) and soy bean (f14)] was determined by in vitro serum IgE testing and skin prick test (SPT). The comparison of the sIgE titers revealed a good concordance between the Centaur and the UniCAP tests for f1, f3, and f13 (94 %, 91 %, and 96 % respectively). However, the concordance was lower for f2, f4, and f14 (76 %, 77 %, and 77 % respectively) because of discrepancies between the two techniques. When compared with SPT and clinical diagnosis, on the 40 discordant cases found between the Centaur and the UniCAP, the Centaur showed concordance with the patients food reaction and SPT in 34/40 cases, and UniCAP in only 6/40 cases. Accordingly, the Centaur test displayed a statistically significantly better performance on specificity and concordance with SPT for f2, f4, and f14 (concordance/specificity = 70%/71%, 76%/75% and 90%/88% respectively), than the CAP test (49%/54%, 51%/52% and 67%/65% respectively).
authors.affiliation: Laboratory of Immunology, CHU Pellegrin, Amélie Raba Léon, Bordeaux, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Contin-Bordes
authors.forename: Anita
authors.initials: A
authors.lastname: Petersen
authors.forename: Isabelle
authors.initials: I
authors.lastname: Chahine
authors.forename: Franck
authors.initials: F
authors.lastname: Boralevi
authors.forename: Hikmat
authors.initials: H
authors.lastname: Chahine
authors.forename: Alain
authors.initials: A
authors.lastname: Taïeb
authors.forename: Anne
authors.initials: A
authors.lastname: Sarrat
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Moreau
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Taupin
mesh_terms: D000293:Adolescent; D000485:Allergens / Q000276:immunology; D000818:Animals; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000175:diagnosis*; D005260:Female; D005512:Food Hypersensitivity / Q000175:diagnosis*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D007073:Immunoglobulin E / Q000097:blood; D007223:Infant; D008163:Luminescent Measurements / Q000295:instrumentation; D008297:Male; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D012882:Skin Tests
chemical_list: D000485:Allergens; D007073:Immunoglobulin E