Creation of intercellular bonds by anchoring protein ligands to membranes using the diphtheria toxin T domain.
We describe the creation of cell adhesion mediated by cell surface engineering. The Flt3-ligand was fused to a membrane anchor made of the diphtheria toxin translocation domain. The fusion protein was attached to the surface of a cell by an acid pulse. Contact with another cell expressing the receptor Flt3 lead to its activation. This activity involved direct cell-cell contact. A mean force of 20 nN was needed to separate functionalized cells after 5 min of contact. Overall, we showed that it is possible to promote specific cell-cell adhesion by attaching protein ligands at the surface of cells.
Perier
Aurélie
A
Commissariat à l'Energie Atomique, DSV, iBiTecS, SIMOPRO, CEA-Saclay, F-91191 Gif sur Yvette, France. aurelieperier@hotmail.com
Gourier
Christine
C
Pichard
Sylvain
S
Husson
Julien
J
Lajeunesse
Evelyne
E
Babon
Aurélie
A
Menez
André
A
Gillet
Daniel
D
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D004167:Diphtheria Toxin / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding
D004167:Diphtheria Toxin; D008024:Ligands; D008565:Membrane Proteins; C084881:flt3 ligand protein