Identification and evaluation of cycling yeast metabolites in two-dimensional comprehensive gas chromatography-time-of-flight-mass spectrometry data.
A yeast metabolome exhibiting oscillatory behavior was analyzed using comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC x GC-TOF-MS) and in-house developed data analysis software methodology, referred to as a signal ratio method (S(ratio) method). In this study, 44 identified unique metabolites were found to exhibit cycling, with a depth-of-modulation amplitude greater than three. After the initial locations are found using the S(ratio) software, and identified preliminarily using ChromaTOF software, the refined mass spectra and peak volumes were subsequently obtained using parallel factor analysis (PARAFAC). The peak volumes provided by PARAFAC deconvolution provide a measurement of the cycling depth-of-modulation amplitude that is more accurate than the initial S(ratio) information (which serves as a rapid screening procedure to find the cycling metabolites while excluding peaks that do not cycle). The S(ratio) reported is a rapid method to determine the depth-of-modulation while not constraining the search to specific cycling frequencies. The phase delay of the cycling metabolites ranged widely in relation to the oxygen consumption cycling pattern.
Mohler
Rachel E
RE
University of Washington, Department of Chemistry, Box 351700, Seattle, WA 98195, USA.
Tu
Benjamin P
BP
Dombek
Kenneth M
KM
Hoggard
Jamin C
JC
Young
Elton T
ET
Synovec
Robert E
RE
D001685:Biological Factors / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D025341:Principal Component Analysis; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
D001685:Biological Factors