Cloning of a novel gene encoding beta-1,3-xylosidase from a marine bacterium, Vibrio sp. strain XY-214, and characterization of the gene product.
The beta-1,3-xylosidase gene (xloA) of Vibrio sp. strain XY-214 was cloned and expressed in Escherichia coli. The xloA gene consisted of a 1,608-bp nucleotide sequence encoding a protein of 535 amino acids with a predicted molecular weight of 60,835. The recombinant beta-1,3-xylosidase hydrolyzed beta-1,3-xylooligosaccharides to D-xylose as a final product.
Umemoto
Yoshiaki
Y
Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie 514-8507, Japan.
Onishi
Ryosuke
R
Araki
Toshiyoshi
T
D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000201:enzymology* / Q000235:genetics*; D043325:Xylan Endo-1,3-beta-Xylosidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism
D004269:DNA, Bacterial; D009844:Oligosaccharides; D011994:Recombinant Proteins; D014994:Xylose; D043325:Xylan Endo-1,3-beta-Xylosidase