Tanshinone IIA downregulates the CD40 expression and decreases MMP-2 activity on atherosclerosis induced by high fatty diet in rabbit.
Tanshinone IIA (Tan IIA) is a member of the major lipophilic components abstracted from the root of Salvia miltiorrhiza Bunge and has the capacity of anti-atherosclerosis. To investigate the potential mechanism, we established an animal model by giving high fatty diet to rabbits and Tan IIA was given in different dose. Then, superoxide dismutase (SOD) activity and the malondialdehyde (MDA) level in serum were detected using spectrophotometry; cluster of differentiation 40 (CD40) expression of cellular membrane fraction of aortas and matrix metalloproteinase-2 (MMP-2) activity of total protein extract of aortas were detected by Western Blotting and Zymography, respectively. Compared with the control group, the level of MDA, the expression of CD40 and the MMP-2 activity were increased while the SOD activity was decreased significantly in model group. After Tan IIA administration, the SOD activity was significantly increased while the level of MDA was decreased; both the expression of CD40 and the activity of MMP-2 were decreased. It is suggested that Tan IIA not only inhibits the oxidation but also suppresses the inflammation in atherosclerotic lesion. Our data suggest that not only anti-oxidation but also anti-inflammation by decrease the expression of CD40 and MMP-2 activity maybe the potential mechanisms by which Tan IIA anti-atherosclerosis.
Fang
Zhi-yuan
ZY
Department of Pharmacology, Xi'an Jiaotong University, School of Medicine, Xi'an, Shaanxi, PR China.
Lin
Rong
R
Yuan
Bing-xiang
BX
Yang
Guang-de
GD
Liu
Yu
Y
Zhang
Hui
H
D045784:Abietanes; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D050197:Atherosclerosis / Q000188:drug therapy* / Q000503:physiopathology; D019013:CD40 Antigens / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004041:Dietary Fats; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000187:drug effects / Q000378:metabolism*; D010616:Phenanthrenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D011817:Rabbits; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism
D045784:Abietanes; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D019013:CD40 Antigens; D004041:Dietary Fats; D010616:Phenanthrenes; C021751:tanshinone; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D020778:Matrix Metalloproteinase 2