Platelet factor-4 is an indicator of blood count recovery in acute myeloid leukemia patients in complete remission.
To investigate whether serum biomarkers can be used to indicate the responsiveness of acute myeloid leukemia to remission induction chemotherapy, we performed MALDI-TOF protein profile analysis of patient sera. The resulting spectra revealed a protein (or peptide) peak at m/z 7764 that varied in intensity; its intensity was much higher in samples from patients in complete remission than in those from patients with resistant disease or in samples taken prior to treatment (at the time of diagnosis). Using fractionation, trypsin digestion, MS/MS, and protein molecular weight analyses, we identified the m/z 7764 protein as platelet factor-4 (PF4). This identification was confirmed by a magnetic bead-based MALDI immunoassay. Statistical comparison of PF4 levels and platelet counts in patient sera revealed a significant positive correlation between the two variables. This study demonstrates that PF4 protein levels are a good indicator for the recovery of blood count in the complete remission of acute myeloid leukemia. The linear positive correlation curve indicates that blood count recovery of platelets to >100,000/mm(3) is equivalent to a serum PF4 recovery level of >2.492 microg/ml.
Kim
Jin Young
JY
Proteome Analysis Team, Korea Basic Science Institute, Daejeon 305-333, Korea.
Song
Ho-Jun
HJ
Lim
Hoi-Jeong
HJ
Shin
Myung-Geun
MG
Kim
Jae Seong
JS
Kim
Hyeoung-Joon
HJ
Kim
Baik Yoon
BY
Lee
Seung-won
SW
D000595:Amino Acid Sequence; D001772:Blood Cell Count; D005591:Chemical Fractionation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007118:Immunoassay; D015470:Leukemia, Myeloid, Acute / Q000097:blood* / Q000188:drug therapy*; D008280:Magnetics; D008297:Male; D008863:Microspheres; D008875:Middle Aged; D008969:Molecular Sequence Data; D010976:Platelet Count; D010978:Platelet Factor 4 / Q000097:blood* / Q000737:chemistry; D012044:Regression Analysis; D012074:Remission Induction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010978:Platelet Factor 4