title: Tissue factor expression on monocytes induced by anti-phospholipid antibodies as a strong risk factor for thromboembolic complications in SLE patients.
abstract: Our aim was to clarify the role of anti-phospholipid antibodies in the pathogenesis of monocyte tissue factor (TF) expression and thromboembolic complications (TE) in patients with SLE. We examined cell surface expression of TF on monocytes in 93 SLE patients. Monocyte TF expression was significantly higher in SLE patients who had TE than in other SLE patients, and confirmed that the high expression of monocyte TF was a strong risk factor for TE. Furthermore, the presence of anti-cardiolipin/beta2-glycoprotein I antibodies (anti-CL/beta2-GPI) was strongly associated with the high expression of monocyte TF. We therefore studied the in vitro effect of IgG anti-CL/beta2-GPI on lipopolysaccharide (LPS)-induced expression of TF on monocytes in healthy peripheral blood and found that purified IgG containing anti-CL/beta2-GPI significantly enhanced LPS-induced monocyte TF expression. These results suggest that anti-CL/beta2-GPI cause persistently high TF expression on monocyte, which may contribute to the risk of thromboembolic events in SLE patients.
authors.affiliation: Laboratory for Clinical Investigation, Osaka University Hospital, Suita, Osaka 565-0871, Japan. nojima@hp-lab.med.osaka-u.ac.jp
authors.forename: Junzo
authors.initials: J
authors.lastname: Nojima
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Masuda
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Iwatani
authors.forename: Etsuji
authors.initials: E
authors.lastname: Suehisa
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Futsukaichi
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Kuratsune
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Toru
authors.initials: T
authors.lastname: Takano
authors.forename: Yoh
authors.initials: Y
authors.lastname: Hidaka
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Kanakura
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D017152:Antibodies, Antiphospholipid / Q000276:immunology / Q000494:pharmacology*; D002648:Child; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000150:complications* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D012307:Risk Factors; D013923:Thromboembolism / Q000209:etiology* / Q000276:immunology; D013925:Thromboplastin / Q000276:immunology / Q000378:metabolism*
chemical_list: D017152:Antibodies, Antiphospholipid; D013925:Thromboplastin