title: Listeriosis in a cockatiel (Nymphicus hollandicus).
abstract: Listeriosis was diagnosed in a 4-yr-old female cockatiel (Nymphicus hollandicus) that died after exhibiting clinical signs that included a fluffed-up appearance, weakness, and loss of weight of several days duration. Grossly, the bird was moderately emaciated, and the liver and spleen were enlarged. Microscopically, there was mild-to-moderate inflammation associated with rod-shaped, gram-positive bacteria in the liver, spleen, kidneys, adrenal glands, bone marrow, and esophagus. Listeria monocytogenes was isolated from the liver, trachea, and intestine. The isolate was identified as type 1 by agglutination with specific antisera, and it further identified as belonging to serovar group 1/2a, 3a by multiplex polymerase chain reaction assay. Listeria monocytogenes also was detected in affected tissues by immunohistochemistry.
authors.affiliation: California Animal Health and Food Safety Laboratory System, Fresno Branch, University of California, Davis, 2789 South Orange Avenue, Fresno, CA 93725, USA. hlshivaprasad@ucdavis.edu
authors.forename: H L
authors.initials: HL
authors.lastname: Shivaprasad
authors.forename: R
authors.initials: R
authors.lastname: Kokka
authors.forename: R L
authors.initials: RL
authors.lastname: Walker
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000382:microbiology*; D001853:Bone Marrow / Q000382:microbiology / Q000473:pathology; D046410:Cockatoos / Q000382:microbiology*; D005260:Female; D008088:Listeriosis / Q000175:diagnosis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D008099:Liver / Q000382:microbiology / Q000473:pathology