Haematopoietic antigen-presenting cells in the human thymic cortex: evidence for a role in selection and removal of apoptotic thymocytes.
Only a small proportion of thymocytes survive T cell selection in the thymus and leave the thymus as mature T cells. The vast majority of thymocytes undergo cell death during selection, either due to failure to undergo positive selection on self peptide-MHC presented by thymic antigen presenting cells (APC) or due to negative selection. In the murine thymus it has been shown that most thymocytes that fail selection undergo apoptosis in the thymic cortex and are removed by cortical macrophages. However, it is unknown how apoptotic thymocytes are cleared from the cortex of the human thymus. Here we report the identification of antigen-presenting cells of haematopoietic origin (hAPCs) by expression of dendritic cell (DC) specific C-type lectin DC-SIGN (CD209) in the cortex of the human thymus, and show that these cells exhibit features of both immature DCs and macrophages. The analysis of cellular markers, in particular the expression of the molecular chaperone HLA-DM, on cortical hAPCs further suggests that these hAPCs may participate in selection of thymocytes in the cortex. Using in situ terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), we demonstrated that these cortical hAPCs are surrounded by apoptotic, TUNEL(+) thymocytes in situ. Futhermore, in situ immuno-cryo-electron microscopy suggests that cortical hAPCs take up and remove apoptotic thymocytes. Thus, DC-SIGN(+) hAPCs in the human thymic cortex appear to function in thymocyte selection and removal of apoptotic thymocytes from the thymic cortex.
Paessens
L C
LC
Department of Molecular Cell Biology and Immunology, VU University Medical Centre, 1081BT, Amsterdam, The Netherlands.
Fluitsma
D M
DM
van Kooyk
Y
Y
D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000648:ultrastructure; D017209:Apoptosis / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002675:Child, Preschool; D003713:Dendritic Cells / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D020287:In Situ Nick-End Labeling / Q000379:methods; D007223:Infant; D037181:Lectins, C-Type / Q000378:metabolism; D008264:Macrophages / Q000276:immunology; D016253:Microscopy, Immunoelectron; D011956:Receptors, Cell Surface / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D013950:Thymus Gland / Q000276:immunology* / Q000648:ultrastructure; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin