title: Optimization of beta-carotene production by Rhodotorula glutinis using high hydrostatic pressure and response surface methodology.
abstract: Rhodotorula glutinis RG6 was treated by high hydrostatic pressure (HHP) of 300 MPa for 15 min for improving its ability of beta-carotene production. After the treatments of 5 repeated cycles, the mutant strain RG6p was obtained, beta-carotene production of which reached 10.01 mg/L, increased by 57.89% compared with 6.34 mg/L from parent strain RG6. To optimize the medium for beta-carotene fermentation by mutant RG6p, a response surface methodology (RSM) approach was used in conjunction with a factorial design and a central composite design, and the maximum yield of beta-carotene (13.43 mg/L), an increase of 34.17% compared to the control, was obtained at a pH 6.7 with an optimum medium (40 mL/250 mL) of yeast extract (4.23 g/L), glucose (12.11 g/L), inoculum (30 mL/L), tomato extract (2.5 mL/L), peanut oil (0.5 mL/L), and (NH(4))(2)SO(4) (5 g/L).
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural Univ., Beijing 100083, P.R. China.
authors.forename: S-L
authors.initials: SL
authors.lastname: Wang
authors.forename: J-S
authors.initials: JS
authors.lastname: Sun
authors.forename: B-Z
authors.initials: BZ
authors.lastname: Han
authors.forename: X-Z
authors.initials: XZ
authors.lastname: Wu
mesh_terms: D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006874:Hydrostatic Pressure; D007218:Industrial Microbiology / Q000379:methods*; D009154:Mutation; D000074241:Peanut Oil; D010938:Plant Oils / Q000378:metabolism; D012248:Rhodotorula / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D019207:beta Carotene / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D000074241:Peanut Oil; D010938:Plant Oils; D019207:beta Carotene; D005947:Glucose