Enzyme-purification and catalytic transformations in a microstructured PASSflow reactor using a new tyrosine-based Ni-NTA linker system attached to a polyvinylpyrrolidinone-based matrix.
The synthesis of a Ni-nitrilotriacetic acid (Ni-NTA) attached via a new tyrosine-based linker matrix on monolithic crosslinked poly(vinyl benzyl chloride)/poly(vinylpyrrolidinone) is described. This matrix is incorporated inside a microstructured PASSflow reactor which was used for automatic purification and immobilisation of His(6)-tagged proteins. These could be used as stable and highly active biocatalysts for the synthesis of (R)-benzoin (6), (R)-2-hydroxy-1-phenylpropan-1-one (7) and 6-O-acetyl-D-glucal (17) in a flow-through mode.
Dräger
Gerald
G
Institut für Organische Chemie and Zentrum für Biomolekulare Wirkstoffchemie (BMWZ), Leibniz Universität Hannover, Schneiderberg 1b, Hannover, Germany. draeger@oci.uni-hannover.de
Kiss
Csilla
C
Kunz
Ulrich
U
Kirschning
Andreas
A
D000446:Aldehyde-Lyases / Q000302:isolation & purification*; D001573:Benzoin / Q000737:chemistry / Q000378:metabolism; D019149:Bioreactors; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D004952:Esters / Q000378:metabolism; D006868:Hydrolysis; D008239:Lysine / Q000378:metabolism; D009532:Nickel / Q000378:metabolism*; D009571:Nitrilotriacetic Acid / Q000378:metabolism*; D011205:Povidone / Q000378:metabolism*; D013237:Stereoisomerism; D014443:Tyrosine / Q000378:metabolism*
D004800:Enzymes, Immobilized; D004952:Esters; D014443:Tyrosine; D009532:Nickel; D000446:Aldehyde-Lyases; C059416:benzaldehyde lyase; D011205:Povidone; D008239:Lysine; D009571:Nitrilotriacetic Acid; D001573:Benzoin