title: The presence of the TAR RNA structure alters the programmed -1 ribosomal frameshift efficiency of the human immunodeficiency virus type 1 (HIV-1) by modifying the rate of translation initiation.
abstract: HIV-1 uses a programmed -1 ribosomal frameshift to synthesize the precursor of its enzymes, Gag-Pol. The frameshift efficiency that is critical for the virus replication, is controlled by an interaction between the ribosome and a specific structure on the viral mRNA, the frameshift stimulatory signal. The rate of cap-dependent translation initiation is known to be altered by the TAR RNA structure, present at the 5' and 3' end of all HIV-1 mRNAs. Depending upon its concentration, TAR activates or inhibits the double-stranded RNA-dependent protein kinase (PKR). We investigated here whether changes in translation initiation caused by TAR affect HIV-1 frameshift efficiency. CD4+ T cells and 293T cells were transfected with a dual-luciferase construct where the firefly luciferase expression depends upon the HIV-1 frameshift. Translation initiation was altered by adding TAR in cis or trans of the reporter mRNA. We show that HIV-1 frameshift efficiency correlates negatively with changes in the rate of translation initiation caused by TAR and mediated by PKR. A model is presented where changes in the rate of initiation affect the probability of frameshifting by altering the distance between elongating ribosomes on the mRNA, which influences the frequency of encounter between these ribosomes and the frameshift stimulatory signal.
authors.affiliation: Département de Biochimie, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Karine
authors.initials: K
authors.lastname: Gendron
authors.forename: Johanie
authors.initials: J
authors.lastname: Charbonneau
authors.forename: Dominic
authors.initials: D
authors.lastname: Dulude
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Heveker
authors.forename: Gerardo
authors.initials: G
authors.lastname: Ferbeyre
authors.forename: Léa
authors.initials: L
authors.lastname: Brakier-Gingras
mesh_terms: D002460:Cell Line; D018965:Frameshifting, Ribosomal / Q000187:drug effects; D015967:Gene Expression Regulation, Viral; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D019169:Jurkat Cells; D008957:Models, Genetic; D010442:Peptide Chain Initiation, Translational / Q000235:genetics; D012315:RNA Caps / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry; D012367:RNA, Viral / Q000737:chemistry; D019892:eIF-2 Kinase / Q000037:antagonists & inhibitors
chemical_list: D012315:RNA Caps; D012333:RNA, Messenger; D012367:RNA, Viral; D019892:eIF-2 Kinase