title: Variation in cytostatic constituents of a sponge-derived Gymnascella dankaliensis by manipulating the carbon source.
abstract: The Halichondria sponge-derived fungus, Gymnacella dankaliensis, was cultured in two different media conditions. A modified malt extract medium containing soluble starch instead of glucose resulted in two extremely unusual steroids, dankasterones A (2) and B (3), while four additional unusual steroids, gymnasterones A (4), B (5), C (6), and D (7), were isolated from the original malt extract medium. Their stereostructures have been established on the basis of spectroscopic analyses along with X-ray crystal structure analyses, modified Mosher's method, CD exciton method, and a chemical transformation. All the steroids except for 4 exhibited significant growth inhibition against the murine P388 cancer cell line. Dankasterone A (2) also exhibited potent growth inhibition against human cancer cell lines.
authors.affiliation: Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. amagata@chemistry.ucsc.edu
authors.forename: Taro
authors.initials: T
authors.lastname: Amagata
authors.forename: Makoto
authors.initials: M
authors.lastname: Tanaka
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamada
authors.forename: Mitsunobu
authors.initials: M
authors.lastname: Doi
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Minoura
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Ohishi
authors.forename: Takao
authors.initials: T
authors.lastname: Yamori
authors.forename: Atsushi
authors.initials: A
authors.lastname: Numata
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D002244:Carbon / Q000737:chemistry; D018360:Crystallography, X-Ray; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007564:Japan; D007941:Leukemia P388; D051379:Mice; D008968:Molecular Conformation; D015394:Molecular Structure; D011161:Porifera / Q000382:microbiology*; D013256:Steroids / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D013256:Steroids; D002244:Carbon