Differential optical spectroscopy for absorption characterization of mono & two-layered scattering media.
Diffuse reflectance techniques are commonly used to characterize the optical properties of various biological tissues in the visible and NIR spectral ranges. Nevertheless a sharp determination of local chromophores concentration in turbid media is usually difficult to obtain because of the nonlinear dependence of the reflected light intensity as a function of scattering and absorption coefficients. The proposed technique of this paper allows to determine absorbent compounds concentration ratios in a turbid medium from three reflectance spectra, measured for source-detector distances inferior to 1 cm. This method is validated in the visible range in case of typical biological absorption and reduced scattering coefficients. Two-layered scattering media are also investigated.
Billet
Cyril
C
CREATIS-LRMN, CNRS UMR 5220, Université de Lyon, France. Cyril.Billet@univ-lyon1.fr
Sablong
Raphael
R
D000042:Absorption; D000818:Animals; D007281:Ink; D008027:Light; D008892:Milk / Q000737:chemistry; D055095:Optics and Photonics; D012542:Scattering, Radiation; D013057:Spectrum Analysis / Q000379:methods