[Effect of Rhizoma coptidis and Radix rehmanniae with different ratio on pharmacokinetics of berberine in rats].
OBJECTIVE
To study the effect of Rhizoma Coptidis and Radix Rehmanniae with the different ratio on the pharmacokinetics of berberine in rats.


METHODS
24 rats were grouped to 4 groups randomly. Decoction, in which the proportion of Rhizoma Coptidis to Radix Rehmanniae is 1:0, 1:1, 1:4, 1:8 differently, was intragastrically given to the 4 groups. HPLC was used to determine concentration of berberine in serum. We adopted DAS 2.0 to analysis pharmacokinetic parameters of berberine.


RESULTS
The concentration-time curves was all fitted to two-compartment model with a weight of 1/C2. Difference of 4groups in C(max), AUC(0-t), AUC(0-infinity), is significant (P <0.05).


CONCLUSIONS
Radix Rehmanniae of large dose can effectively enhance berberine's bioavailability in rats.
Wang
Xiao-qiang
XQ
Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Yang
Ming-wei
MW
Liu
Fang-fang
FF
Lu
Fu-er
FE
Xu
Li-jun
LJ
Zou
Xin
X
D000818:Animals; D019540:Area Under Curve; D001599:Berberine / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D001682:Biological Availability; D039323:Coptis / Q000737:chemistry*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics*; D008297:Male; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D032267:Rehmannia / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D001599:Berberine