Synthesis and cytotoxicity evaluation of 22,23-oxygenated stigmastane derivatives.
Starting from (22E)-3alpha,5alpha-cyclo-6beta-methoxystigmast-22-ene eighteen derivatives of (22S,23S)-22,23-oxidostigmastane, (22R,23R)-22,23-oxidostigmastane, and (22R,23R)-22,23-dihydroxystigmastane were synthesized and screened for cytotoxicity in human hepatoma Hep G2 cells and human breast carcinoma MCF-7 cells using MTT assay. Four compounds of this series exhibited high cytotoxicity in both cells; three compounds were selectively toxic in MCF-7 cells, one compound was toxic in Hep G2 cells, rather than in MCF-7 cells; four compounds at low concentrations increased MTT test values over the control.
Misharin
Alexander Yu
AY
V.N. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia.
Mehtiev
Arif R
AR
Morozevich
Galina E
GE
Tkachev
Yaroslav V
YV
Timofeev
Vladimir P
VP
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002783:Cholestenones / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity*; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D010100:Oxygen / Q000737:chemistry*; D011522:Protons; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
D002783:Cholestenones; D011522:Protons; D010100:Oxygen