Arenibacter echinorum sp. nov., isolated from the sea urchin Strongylocentrotus intermedius.
Two marine, heterotrophic, aerobic, pigmented and gliding bacteria, isolated from the sea urchin Strongylocentrotus intermedius were subjected to a polyphasic taxonomy study. The 16S rRNA gene sequence analysis revealed that strains KMM 6032T and KMM 6047 formed a distinct branch within the genus Arenibacter, a member of the family Flavobacteriaceae. The level of sequence similarity between the novel isolates and members of the genus Arenibacter was 94.5-98.9%. The DNA G+C content was 39-40 mol%. The dominant fatty acids were iso-C15:1, iso-C15:0, C15:0, C15:1omega6c, iso-C15:0 3-OH, iso-C17:1omega9c, iso-C17:0 3-OH and summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1omega7c). The results of DNA-DNA hybridization experiments supported by phenotypic data indicated that the isolates represent a novel species within the genus Arenibacter, for which the name Arenibacter echinorum sp. nov. is proposed. The type strain is KMM 6032T (=KCTC 22013T=LMG 22574T).
Nedashkovskaya
Olga I
OI
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia.
Kim
Seung Bum
SB
Department of Microbiology, Chungnam National University, 220 Gung-dong, Yuseong, Daejeon 305-764, Republic of Korea.
Lysenko
Anatoly M
AM
Institute of Microbiology of the Russian Academy of Sciences, Pr. 60 let October 7/2, Moscow 117811, Russia.
Lee
Kang Hyun
KH
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Oun-dong, Yuseong, Daejeon 305-333, Republic of Korea.
Bae
Kyung Sook
KS
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Oun-dong, Yuseong, Daejeon 305-333, Republic of Korea.
Mikhailov
Valery V
VV
Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia.
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D047269:Strongylocentrotus / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S