Neuroprotective effect of long-term MgSO4 administration after cerebral hypoxia-ischemia in newborn rats is related to the severity of brain damage.
Previous studies have shown contradictory results regarding magnesium-mediated neuroprotection in animal models of perinatal asphyxia. The aim of this study is to investigate the effects of MgSO(4) postasphyxial treatment on hypoxia-ischemia (HI)-induced brain injury in neonatal rats and the possibility that this effect is related to the severity of brain damage. Seven-day-old rats underwent unilateral carotid artery ligation followed by 1 or 2 hours of hypoxia (8% O(2)) and MgSO(4) administration. Adenosine triphosphate/phosphocreatine and glutamate/glutamine measurements and neuropathological evaluation of the hippocampus were used to assess the effects of HI and MgSO(4). HI caused time-dependent changes in energy stores, amino acid concentrations, and brain damage. Administration of MgSO(4) after 1 hour but not after 2 hours of hypoxia resulted in significant prevention of HI-induced brain injury. MgSO(4) administration results in a significant protection against moderate HI-induced brain damage, whereas it fails to offer a similar effect against severe brain damage.
Spandou
Evangelia
E
Department of Physiology and Pharmacology, Faculty of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece. spandou@med.auth.gr
Soubasi
Vassiliki
V
Papoutsopoulou
Stamatia
S
Augoustides-Savvopoulou
Persefoni
P
Loizidis
Theodoros
T
Pazaiti
Anastasia
A
Karkavelas
George
G
Guiba-Tziampiri
Olympia
O
D000255:Adenosine Triphosphate / Q000378:metabolism; D000367:Age Factors; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D005260:Female; D006624:Hippocampus / Q000473:pathology; D002534:Hypoxia, Brain / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D020925:Hypoxia-Ischemia, Brain / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008278:Magnesium Sulfate / Q000494:pharmacology*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010725:Phosphocreatine / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D000596:Amino Acids; D018696:Neuroprotective Agents; D010725:Phosphocreatine; D008278:Magnesium Sulfate; D000255:Adenosine Triphosphate