A chromogenic substrate culture plate for early identification of Vibrio vulnificus and isolation of other marine Vibrios.
Vibrio vulnificus infection can result in necrotizing fasciitis and sepsis, which have short latentcy periods and high mortality rates. Thus, an easy and quick detection method is needed to improve the outcome. To distinguish V. vulnificus from other pathogens that cause necrotizing fasciitis, we developed a selective isolation culture agar plate (Chromochecker Vibrio Agar-1; CVA-1) for use in environmental monitoring and in the clinical setting. One hundred four strains of V. vulnificus, already identified biochemically, showed typical colony form and color when grown on CVA-1. Thirty-six of 51 marine bacteria samples suspected to be V. vulnificus on CVA-1 were subsequently identified as V. vulnificus by a biochemical identification system. Of 8 bacteria known to cause necrotizing fasciitis, only V. vulnificus grew on CVA-1. In addition, growth on CVA-1 allowed ready differentiation of Vibrio species. CVA-1 can be used to distinguish pathogenic Vibrios according to colony form and chromatic differences.
Nakashima
Yukari
Y
Department of Clinical Laboratory Medicine, Saga University Hospital, Saga, Japan.
Oho
Megumi
M
Kusaba
Kouji
K
Nagasawa
Zenzo
Z
Komatsu
Osamu
O
Manome
Isao
I
Araki
Kazukuni
K
Oishi
Hirotaka
H
Nakashima
Mikio
M
D000368:Aged; D000818:Animals; D002863:Chromogenic Compounds; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003470:Culture Media / Q000592:standards*; D006801:Humans; D008297:Male; D010125:Ostreidae / Q000382:microbiology; D012623:Seawater / Q000382:microbiology*; D013997:Time Factors; D014733:Vibrio / Q000145:classification / Q000302:isolation & purification*; D014735:Vibrio Infections / Q000175:diagnosis*; D041261:Vibrio vulnificus / Q000302:isolation & purification*
D002863:Chromogenic Compounds; D003470:Culture Media