title: Electrical detection of fast reaction kinetics in nanochannels with an induced flow.
abstract: Nanofluidic channels can be used to enhance surface binding reactions, since the target molecules are closely confined to the surfaces that are coated with specific binding partners. Moreover, diffusion-limited binding can be significantly enhanced if the molecules are steered into the nanochannels via either pressure-driven or electrokinetic flow. By monitoring the nanochannel impedance, which is sensitive to surface binding, low analyte concentrations have been detected electrically in nanofluidic channels within response times of 1-2 h. This represents a approximately 54 fold reduction in the response time using convective flow compared to diffusion-limited binding. At high-flow velocities, the presented method of reaction kinetics enhancement is potentially limited by force-induced dissociations of the receptor-ligand bonds. Optimization of this scheme could be useful for label-free, electrical detection of biomolecule binding reactions within nanochannels on a chip.
authors.affiliation: Department of Electrical Engineering and Computer Science, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Reto B
authors.initials: RB
authors.lastname: Schoch
authors.forename: Lih Feng
authors.initials: LF
authors.lastname: Cheow
authors.forename: Jongyoon
authors.initials: J
authors.lastname: Han
mesh_terms: D001710:Biotin; D004563:Electrochemistry / Q000379:methods; D007700:Kinetics; D008958:Models, Molecular; D043942:Nanotubes / Q000737:chemistry*; D011092:Polyethylene Glycols; D019809:Streptavidin / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011092:Polyethylene Glycols; D001710:Biotin; D019809:Streptavidin