In situ proteolysis for protein crystallization and structure determination.
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
Dong
Aiping
A
Structural Genomics Consortium, University of Toronto, 100 College Street, Toronto, Ontario M5G 1L5, Canada.
Xu
Xiaohui
X
Edwards
Aled M
AM
Chang
Changsoo
C
Chruszcz
Maksymilian
M
Cuff
Marianne
M
Cymborowski
Marcin
M
Di Leo
Rosa
R
Egorova
Olga
O
Evdokimova
Elena
E
Filippova
Ekaterina
E
Gu
Jun
J
Guthrie
Jennifer
J
Ignatchenko
Alexandr
A
Joachimiak
Andrzej
A
Klostermann
Natalie
N
Kim
Youngchang
Y
Korniyenko
Yuri
Y
Minor
Wladek
W
Que
Qiuni
Q
Savchenko
Alexei
A
Skarina
Tatiana
T
Tan
Kemin
K
Yakunin
Alexander
A
Yee
Adelinda
A
Yim
Veronica
V
Zhang
Rongguang
R
Zheng
Hong
H
Akutsu
Masato
M
Arrowsmith
Cheryl
C
Avvakumov
George V
GV
Bochkarev
Alexey
A
Dahlgren
Lars-Göran
LG
Dhe-Paganon
Sirano
S
Dimov
Slav
S
Dombrovski
Ludmila
L
Finerty
Patrick
P
Flodin
Susanne
S
Flores
Alex
A
Gräslund
Susanne
S
Hammerström
Martin
M
Herman
Maria Dolores
MD
Hong
Bum-Soo
BS
Hui
Raymond
R
Johansson
Ida
I
Liu
Yongson
Y
Nilsson
Martina
M
Nedyalkova
Lyudmila
L
Nordlund
Pär
P
Nyman
Tomas
T
Min
Jinrong
J
Ouyang
Hui
H
Park
Hee-won
HW
Qi
Chao
C
Rabeh
Wael
W
Shen
Limin
L
Shen
Yang
Y
Sukumard
Deepthi
D
Tempel
Wolfram
W
Tong
Yufeng
Y
Tresagues
Lionel
L
Vedadi
Masoud
M
Walker
John R
JR
Weigelt
Johan
J
Welin
Martin
M
Wu
Hong
H
Xiao
Ting
T
Zeng
Hong
H
Zhu
Haizhong
H
D003460:Crystallization / Q000379:methods*; D003461:Crystallography / Q000379:methods*; D010447:Peptide Hydrolases / Q000737:chemistry*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure*
D011506:Proteins; D010447:Peptide Hydrolases