title: Low-density lipoprotein (LDL)-antioxidant lignans from Myristica fragrans seeds.
abstract: Six diarylbutane lignans 1-5 and one aryltetralin lignan 6 were isolated from the methanol (95%) extracts of Myristica fragrans seeds and then 7-methyl ether diarylbutane lignan 4 has proven to be new a compound. Their compounds 1-7 were evaluated for LDL-antioxidant activity to identify the most potent LDL-antioxidant 3 with an IC50 value of 2.6 microM in TBARS assay. Due to its potency, compound 3 was tested for complementary in vitro investigations, such as lag time (140 min at 1.0 microM), relative electrophoretic mobility (REM) of ox-LDL (inhibition of 80% at 20 microM and 72% at 10 microM), and fragmentation of apoB-100 (inhibition of 93% at 20 microM) on copper-mediated LDL oxidation. In macrophage-mediated LDL oxidation, the TBARS formation was also inhibited by compound 3.
authors.affiliation: Division of Applied Life Science (BK21 Program), EB-NCRC, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Hyun Sook
authors.initials: HS
authors.lastname: Kwon
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Hyung Jae
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Min Suk
authors.initials: MS
authors.lastname: Yang
authors.forename: Ki Hun
authors.initials: KH
authors.lastname: Park
authors.forename: Tae-Sook
authors.initials: TS
authors.lastname: Jeong
authors.forename: Woo Song
authors.initials: WS
authors.lastname: Lee
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D053299:Apolipoprotein B-100 / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor; D003300:Copper / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000737:chemistry* / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D026323:Myristica / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry; D017392:Thiobarbituric Acid Reactive Substances / Q000737:chemistry / Q000378:metabolism
chemical_list: D000975:Antioxidants; D053299:Apolipoprotein B-100; D017705:Lignans; D008077:Lipoproteins, LDL; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D003300:Copper