title: Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA-microgel bioassays.
abstract: We demonstrate that DNA oligonucleotides covalently coupled to colloidal microgel can be manipulated by T4 DNA ligase for DNA ligation and by Phi29 DNA polymerase for rolling circle amplification (RCA). We also show that the long single-stranded RCA product can generate intensive fluorescence upon hybridization with complementary fluorescent DNA probe. We believe DNA-microgel conjugates can be explored for the development of DNA based bioassays and biosensors.
authors.affiliation: Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main St. W., Hamilton, ON, L8N 3Z5, Canada.
authors.forename: Md Monsur
authors.initials: MM
authors.lastname: Ali
authors.forename: Shunxing
authors.initials: S
authors.lastname: Su
authors.forename: Carlos D M
authors.initials: CD
authors.lastname: Filipe
authors.forename: Robert
authors.initials: R
authors.lastname: Pelton
authors.forename: Yingfu
authors.initials: Y
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D001681:Biological Assay / Q000379:methods; D015374:Biosensing Techniques / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D011088:DNA Ligases / Q000737:chemistry* / Q000378:metabolism; D015342:DNA Probes / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism; D005782:Gels / Q000737:chemistry*; D008969:Molecular Sequence Data
chemical_list: D015342:DNA Probes; D005782:Gels; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D011088:DNA Ligases