title: Multivalent binding of small guest molecules and proteins to molecular printboards inside microchannels.
abstract: Beta-Cyclodextrin (beta-CD) monolayers have been immobilized in microchannels. The host-guest interactions on the beta-CD monolayers inside the channels were comparable to the interactions on beta-CD monolayers on planar surfaces, and a divalent fluorescent guest attached with a comparable binding strength. Proteins were attached to these monolayers inside microchannels in a selective manner by employing a strategy that uses streptavidin and orthogonal linker molecules. The design of the chip, which involved a large channel that splits into four smaller channels, allowed the channels to be addressed separately and led to the selective immobilization of antibodies. Experiments with labeled antibodies showed the selective immobilization of these antibodies in the separate channels.
authors.affiliation: Molecular Nanofabrication group MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
authors.forename: Manon J W
authors.initials: MJ
authors.lastname: Ludden
authors.forename: Xing Yi
authors.initials: XY
authors.lastname: Ling
authors.forename: Tian
authors.initials: T
authors.lastname: Gang
authors.forename: Wojciech P
authors.initials: WP
authors.lastname: Bula
authors.forename: Han J G E
authors.initials: HJ
authors.lastname: Gardeniers
authors.forename: David N
authors.initials: DN
authors.lastname: Reinhoudt
authors.forename: Jurriaan
authors.initials: J
authors.lastname: Huskens
mesh_terms: D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry; D014867:Water / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D011506:Proteins; D012235:Rhodamines; D047392:beta-Cyclodextrins; D014867:Water