title: A chromogenic substrate culture plate for early identification of Vibrio vulnificus and isolation of other marine Vibrios.
abstract: Vibrio vulnificus infection can result in necrotizing fasciitis and sepsis, which have short latentcy periods and high mortality rates. Thus, an easy and quick detection method is needed to improve the outcome. To distinguish V. vulnificus from other pathogens that cause necrotizing fasciitis, we developed a selective isolation culture agar plate (Chromochecker Vibrio Agar-1; CVA-1) for use in environmental monitoring and in the clinical setting. One hundred four strains of V. vulnificus, already identified biochemically, showed typical colony form and color when grown on CVA-1. Thirty-six of 51 marine bacteria samples suspected to be V. vulnificus on CVA-1 were subsequently identified as V. vulnificus by a biochemical identification system. Of 8 bacteria known to cause necrotizing fasciitis, only V. vulnificus grew on CVA-1. In addition, growth on CVA-1 allowed ready differentiation of Vibrio species. CVA-1 can be used to distinguish pathogenic Vibrios according to colony form and chromatic differences.
authors.affiliation: Department of Clinical Laboratory Medicine, Saga University Hospital, Saga, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Nakashima
authors.forename: Megumi
authors.initials: M
authors.lastname: Oho
authors.forename: Kouji
authors.initials: K
authors.lastname: Kusaba
authors.forename: Zenzo
authors.initials: Z
authors.lastname: Nagasawa
authors.forename: Osamu
authors.initials: O
authors.lastname: Komatsu
authors.forename: Isao
authors.initials: I
authors.lastname: Manome
authors.forename: Kazukuni
authors.initials: K
authors.lastname: Araki
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Oishi
authors.forename: Mikio
authors.initials: M
authors.lastname: Nakashima
mesh_terms: D000368:Aged; D000818:Animals; D002863:Chromogenic Compounds; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003470:Culture Media / Q000592:standards*; D006801:Humans; D008297:Male; D010125:Ostreidae / Q000382:microbiology; D012623:Seawater / Q000382:microbiology*; D013997:Time Factors; D014733:Vibrio / Q000145:classification / Q000302:isolation & purification*; D014735:Vibrio Infections / Q000175:diagnosis*; D041261:Vibrio vulnificus / Q000302:isolation & purification*
chemical_list: D002863:Chromogenic Compounds; D003470:Culture Media