title: Long-term clinical protection from falciparum malaria is strongly associated with IgG3 antibodies to merozoite surface protein 3.
abstract: BACKGROUND
  Surrogate markers of protective immunity to malaria in humans are needed to rationalize malaria vaccine discovery and development. In an effort to identify such markers, and thereby provide a clue to the complex equation malaria vaccine development is facing, we investigated the relationship between protection acquired through exposure in the field with naturally occurring immune responses (i.e., induced by the parasite) to molecules that are considered as valuable vaccine candidates.
  
  
  RESULTS
  We analyzed, under comparative conditions, the antibody responses of each of six isotypes to five leading malaria vaccine candidates in relation to protection acquired by exposure to natural challenges in 217 of the 247 inhabitants of the African village of Dielmo, Senegal (96 children and 121 older adolescents and adults). The status of susceptibility or resistance to malaria was determined by active case detection performed daily by medical doctors over 6 y from a unique follow-up study of this village. Of the 30 immune responses measured, only one, antibodies of the IgG3 isotype directed to merozoite surface protein 3 (MSP3), was strongly associated with clinical protection against malaria in all age groups, i.e., independently of age. This immunological parameter had a higher statistical significance than the sickle cell trait, the strongest factor of protection known against Plasmodium falciparum. A single determination of antibody was significantly associated with the clinical outcome over six consecutive years in children submitted to massive natural parasite challenges by mosquitoes (over three parasite inoculations per week). Finally, the target epitopes of these antibodies were found to be fully conserved.
  
  
  CONCLUSIONS
  Since anti-MSP3 IgG3 antibodies can naturally develop along with protection against P. falciparum infection in young children, our results provide the encouraging indication that these antibodies should be possible to elicit by vaccination early in life. Since these antibodies have been found to achieve parasite killing under in vitro and in vivo conditions, and since they can be readily elicited by immunisation in naïve volunteers, our immunoepidemiological findings support the further development of MSP3-based vaccine formulations.
authors.affiliation: Bio-Medical Parasitology Unit, Institut Pasteur, Paris, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Roussilhon
authors.forename: Claude
authors.initials: C
authors.lastname: Oeuvray
authors.forename: Christine
authors.initials: C
authors.lastname: Müller-Graf
authors.forename: Adama
authors.initials: A
authors.lastname: Tall
authors.forename: Christophe
authors.initials: C
authors.lastname: Rogier
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Trape
authors.forename: Michael
authors.initials: M
authors.lastname: Theisen
authors.forename: Aissatou
authors.initials: A
authors.lastname: Balde
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Pérignon
authors.forename: Pierre
authors.initials: P
authors.lastname: Druilhe
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D007223:Infant; D007231:Infant, Newborn; D017780:Malaria Vaccines / Q000235:genetics / Q000276:immunology; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000276:immunology*; D008297:Male; D052940:Merozoites / Q000276:immunology; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D012675:Senegal / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D016036:Seroepidemiologic Studies
chemical_list: D000953:Antigens, Protozoan; D000939:Epitopes; D007074:Immunoglobulin G; D017780:Malaria Vaccines; D015800:Protozoan Proteins; C089133:merozoite surface protein 3, Plasmodium