title: Halorubrum ejinorense sp. nov., isolated from Lake Ejinor, Inner Mongolia, China.
abstract: A novel halophilic archaeon, strain EJ-32T, was isolated from water from Lake Ejinor in Inner Mongolia, China. The taxonomy of strain EJ-32T was studied by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities, strain EJ-32T was shown to be phylogenetically related to Halorubrum coriense (97.9%), Halorubrum trapanicum (97.9%), Halorubrum sodomense (97.8%), Halorubrum tebenquichense (97.8%), Halorubrum xinjiangense (97.6%), Halorubrum terrestre (97.4%), Halorubrum distributum (97.1%) and Halorubrum saccharovorum (96.4%). Strain EJ-32T was found to be neutrophilic, non-motile and Gram-negative. It grew in medium containing saturation concentrations of NaCl and did not require magnesium for optimal growth. The G+C content of the DNA is 64.0 mol%. Values for DNA-DNA hybridization with respect to phylogenetically related Halorubrum species were <or=49%, indicating that EJ-32T constitutes a different genospecies. The data show that strain EJ-32T represents a novel species of the genus Halorubrum, for which the name Halorubrum ejinorense sp. nov. is proposed. The type strain is EJ-32T (=CECT 7194T=CGMCC 1.6782T=JCM 14265T).
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
authors.forename: A M
authors.initials: AM
authors.lastname: Castillo
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Gutiérrez
authors.affiliation: Noda Institute for Scientific Research, 399 Noda, Noda-shi, Chiba-ken 278-0037, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kamekura
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Biotechnology, University of the Western Cape, Bellville 7535, Cape Town, South Africa.
authors.forename: D A
authors.initials: DA
authors.lastname: Cowan
authors.affiliation: Genencor International BV, Archimedesweg 30, 2333 CN Leiden, The Netherlands.
authors.forename: B E
authors.initials: BE
authors.lastname: Jones
authors.affiliation: Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, UK.
authors.forename: W D
authors.initials: WD
authors.lastname: Grant
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Ventosa
mesh_terms: D002681:China; D019641:DNA, Archaeal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D005838:Genotype; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride