title: [Effects of vitamin E and selenium on the metabolism of free radicals in broilers].
abstract: Taking 200 healthy broilers at 14 d of age as test materials, the free radicals in their blood and tissues were detected by electron spin resonance (ESR) and biochemical methods, aimed to investigate the effects of vitamin E (V(E)) and selenium (Se) on the metabolism of different free radicals and their dynamic changes in the broilers. The results showed that the content of NO free radicals in broilers tissues decreased with increasing supplementing level of V(E), while high supplementation of Se tended to induce the production of NO free radicals. High supplementation of V(E) and Se in feeds improved the GSH-Px and SOD activities in broilers serum and liver significantly. With the extension of experimental period, the SOD activity in tissues decreased, while GSH-PX activity increased gradually, implying that the deficiency of V(E) and/or Se might induce the overproduction of O2*- and H2O2 free radicals. H2O2 free radicals might be produced largely at early stage of V(E) and Se deficiency and declined then, while the over-production of O2*- free radicals could maintain for a long time. The deficiency of V(E) and/or Se could improve the MDA content significantly, and Se deficiency had higher effects than V(E) deficiency. There were synergic effects in the metabolism of NO, O2 and H2O2 free radicals.
authors.affiliation: College of Biology and Agriculture, Shanghai Jiaotong University, Shanghai 201101, China. jxxu1962@sjtu.edu.cn
authors.forename: Jian-Xiong
authors.initials: JX
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Tian
authors.initials: T
authors.lastname: Wang
mesh_terms: D000818:Animals; D002645:Chickens / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D005609:Free Radicals / Q000097:blood / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000097:blood / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000097:blood / Q000378:metabolism; D018036:Selenium Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology*; D014815:Vitamins / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D005609:Free Radicals; D017382:Reactive Oxygen Species; D018036:Selenium Compounds; D014815:Vitamins; D014810:Vitamin E; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase