title: Superparamagnetic gamma-Fe(2)O(3) nanoparticles with tailored functionality for protein separation.
abstract: Polymer coated superparamagnetic gamma-Fe(2)O(3) nanoparticles were derivatized with a synthetic double-stranded RNA [poly(IC)], a known allosteric activator of the latent (2-5)A synthetase, to separate a single 35 kDa protein from a crude extract which cross reacted with antibodies raised against the sponge enzyme.
authors.affiliation: Institut für Anorganische Chemie und Analytische Chemie, Universität Mainz, Duesbergweg 10-14, D-55099, Mainz, Germany.
authors.forename: Mohammed Ibrahim
authors.initials: MI
authors.lastname: Shukoor
authors.forename: Filipe
authors.initials: F
authors.lastname: Natalio
authors.forename: Muhammad Nawaz
authors.initials: MN
authors.lastname: Tahir
authors.forename: Vadim
authors.initials: V
authors.lastname: Ksenofontov
authors.forename: Helen Annal
authors.initials: HA
authors.lastname: Therese
authors.forename: Patrick
authors.initials: P
authors.lastname: Theato
authors.forename: Heinz C
authors.initials: HC
authors.lastname: Schröder
authors.forename: Werner E G
authors.initials: WE
authors.lastname: Müller
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Tremel
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D001665:Binding Sites; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D018189:Immunomagnetic Separation; D008024:Ligands; D008280:Magnetics; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*; D011161:Porifera / Q000201:enzymology; D013499:Surface Properties
chemical_list: D005290:Ferric Compounds; D008024:Ligands; D010455:Peptides; C000499:ferric oxide; D012321:DNA-Directed RNA Polymerases; D015088:2',5'-Oligoadenylate Synthetase