title: A novel point of care diagnostic device: impedimetric detection of a biomarker in whole blood.
abstract: There is an unmet medical need for a more reliable and earlier assessment of patients suspected of having a deep vein thrombosis. We describe a novel approach which is developing a highly reliable, accurate, portable and handheld prototype medical diagnostic device to improve radically the speed, accuracy and reliability with which DVT and related blood clotting conditions can be assessed. The device will measure whole blood concentration of D-dimer, a recognized biomarker of increased blood clotting activity, and through innovation in the development of a novel detection, measurement and reporting system, will offer the opportunity to use the test in the point of care setting. The device combines innovation in antibody bio-engineering for high specificity immunoassay-based diagnostics and nano/micro engineered impedimetric analysis electrodes incorporating a biocompatible polymer substrate with development of a disposable microfluidic manifold specifically enabling diagnostics at the point-of-first-contact.
authors.affiliation: Cenamps, The Centre of Excellence in Nano and Micro Photonic Systems, Fabriam Centre, Newcastle upon Tyne, NE28 9NZ, UK. amanda.mcmurray@cenamps.com
authors.forename: Amanda A
authors.initials: AA
authors.lastname: McMurray
authors.forename: Zulfiqur
authors.initials: Z
authors.lastname: Ali
authors.forename: Jan
authors.initials: J
authors.lastname: Kyselovik
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Mills
authors.forename: Nicole Jaffrezic
authors.initials: NJ
authors.lastname: Renault
authors.forename: Hunor
authors.initials: H
authors.lastname: Santha
authors.forename: Christof
authors.initials: C
authors.lastname: Strohhöfer
mesh_terms: D000918:Antibody Specificity; D001672:Biocompatible Materials / Q000737:chemistry; D015415:Biomarkers / Q000097:blood; D017097:Electric Impedance; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D019095:Point-of-Care Systems; D011108:Polymers / Q000737:chemistry; D020246:Venous Thrombosis / Q000097:blood* / Q000175:diagnosis
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D005338:Fibrin Fibrinogen Degradation Products; D011108:Polymers; C036309:fibrin fragment D