Quantifying small numbers of antibodies with a 'near-universal' protein-DNA chimera.
We present general means to greatly increase the sensitivity of antibody-based assays. Augmentation relies on a 'tadpole' protein-DNA chimera whose protein moiety binds most classes of mammalian antibodies but not avian immunoglobulin Y (IgY). We used this tadpole in affinity capture assays followed by real-time PCR to quantify numerous molecules, including prostate-specific antigen (PSA) in human serum, with great sensitivity and accuracy.
Burbulis
Ian
I
Center for Quantitative Genome Function and Molecular Sciences Institute, 2168 Shattuck Avenue, Berkeley, California 94704, USA.
Yamaguchi
Kumiko
K
Yu
Richard
R
Resnekov
Orna
O
Brent
Roger
R
D004247:DNA / Q000276:immunology; D004268:DNA-Binding Proteins / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008832:Microchemistry / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D004247:DNA; D017430:Prostate-Specific Antigen