pH sensing in living cells using fluorescent microspheres.
Intracellular pH in living cells is measured in real time at the single cell level using fluorescently covalently loaded microspheres as efficient carrier systems and stable sensors. The use of these sensors immobilized covalently onto polymeric particles allows analysis of intracellular pH flux over long period of time and eliminates the disadvantages such as dilution within the cell, elimination via leakage or compartmentalization.
Bradley
Mark
M
Chemical Biology Section, School of Chemistry, University of Edinburgh, Joseph Black Building, West Mains Road, EH9 3JJ Edinburgh, United Kingdom.
Alexander
Lois
L
Duncan
Karen
K
Chennaoui
Mourad
M
Jones
Anita C
AC
Sánchez-Martín
Rosario M
RM
D002460:Cell Line; D005434:Flow Cytometry / Q000379:methods*; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence / Q000379:methods*; D008863:Microspheres; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D019793:Fluorescein