title: Decrease of hirudin degradation by deleting the KEX1 gene in recombinant Pichia pastoris.
abstract: Our previous study on recombinant hirudin production in Pichia pastoris demonstrated that, although the total productivity of hirudin was fairly high, its degradation was still severe, even if many engineering methods were applied to improve cell viability and reduce the release of intracellular proteinases. In this work, a pop-in/pop-out method, replacing the auxotrophic marker ARG4 gene with the resistant marker sh ble gene, was used to delete the KEX1 gene to reduce hirudin degradation in P. pastoris GS115Hir. Using this strategy, hirudin degradation was greatly decreased. At the same wet cell weight and cell viability, the percentage of intact hirudin Hir65 in total hirudin in strain GS115HirDeltakex1 was always kept as high as 90% in the initial stage of the methanol fermentation phase and above 62% even in the later stage of the methanol fermentation phase, whereas the percentage for the undeleted strain GS115Hir was only about 40% in the whole methanol fermentation phase. As a result, the intact hirudin Hir65 concentration could maximally reach 2.4 g/l in GS115HirDeltakex1 while it was only 1.1 g/l in GS115Hir.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Ni
authors.forename: Xiangshan
authors.initials: X
authors.lastname: Zhou
authors.forename: Xueqian
authors.initials: X
authors.lastname: Sun
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuanxing
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D002268:Carboxypeptidases / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics; D005285:Fermentation; D017353:Gene Deletion; D006629:Hirudins / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods; D016254:Mutagenesis, Insertional; D010843:Pichia / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D006629:Hirudins; D011994:Recombinant Proteins; D002268:Carboxypeptidases