Flavobacterium ceti sp. nov., isolated from beaked whales (Ziphius cavirostris).
Three isolates of a Gram-negative, catalase- and oxidase-positive, rod-shaped bacterium, isolated from the lung and liver of two beaked whales, were characterized by phenotypic and molecular genetic methods. Based on cellular morphology and biochemical criteria, the isolates were tentatively assigned to the family Flavobacteriaceae, although they did not appear to correspond to any recognized species. Comparative 16S rRNA gene sequencing showed that the three new isolates shared 100% sequence similarity. The unknown bacterium was phylogenetically closely related to, but distinct from the type strains of Flavobacterium johnsoniae (93.7% sequence similarity), Flavobacterium frigidimaris (93.4%), Flavobacterium aquidurense (93.4%), Flavobacterium hibernum (93.4%) and Flavobacterium degerlachei (93.4%). The novel isolates were readily distinguished from these and other related Flavobacterium species by physiological and biochemical tests. On the basis of phenotypic and phylogenetic evidence, it is proposed that the unknown isolates from whales are classified as a novel species of the genus Flavobacterium, Flavobacterium ceti sp. nov. The type strain is 454-2T (=CECT 7184T=CCUG 52969T).
Vela
A I
AI
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
Fernandez
A
A
Departamento de Anatomía Patológica, Instituto Universitario de Sanidad Animal, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, 35016 Gran Canaria, Spain.
Sánchez-Porro
C
C
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain.
Sierra
E
E
Departamento de Anatomía Patológica, Instituto Universitario de Sanidad Animal, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, 35016 Gran Canaria, Spain.
Mendez
M
M
Departamento de Anatomía Patológica, Instituto Universitario de Sanidad Animal, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, 35016 Gran Canaria, Spain.
Arbelo
M
M
Departamento de Anatomía Patológica, Instituto Universitario de Sanidad Animal, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, 35016 Gran Canaria, Spain.
Ventosa
A
A
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain.
Domínguez
L
L
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
Fernández-Garayzábal
J F
JF
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
D000818:Animals; D015373:Bacterial Typing Techniques; D004275:DNA, Ribosomal / Q000032:analysis; D045826:Flavobacteriaceae Infections / Q000382:microbiology / Q000662:veterinary*; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D020459:Genes, rRNA; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014907:Whales / Q000382:microbiology*
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S