title: The expression of myoglobin and ROR2 protein in Dupuytren's disease.
abstract: BACKGROUND
  Dupuytren's disease (DD) is a hand disease inherited as an autosomal dominant trait with variable penetrance, especially among populations of northern European ancestry. The etiology and pathophysiology of DD are not clear. The purpose of this study was to examine the gene expression profiles of palmar fascia of DD and healthy patients using microarray analysis to highlight the genes that might contribute to the pathogenesis of DD.
  
  
  METHODS
  Dupuytren contracture samples were taken from excised mature cords of DD patients during aponeurectomies. Control samples were collected from healthy hand trauma patients. Microarray analysis was performed with the Affymetrix HGU133A genome array (Affymetrix, Santa Clara, CA). Expression changes of selected proteins were confirmed at the protein level with Western and dot blotting or by immunohistochemistry.
  
  
  RESULTS
  At least an 8-fold change in gene expression was found with 127 genes, including a 90-fold down-regulation of myoglobin and a 14-fold up-regulation of tyrosine kinase-like orphan receptor 2 (= ROR2) from absent to present during the disease. The changes in myoglobin and ROR2 expression were confirmed at the protein level.
  
  
  CONCLUSIONS
  In this study, we showed for the first time the connection of ROR2 in Dupuytren's disease. ROR2 and myoglobin may play an important role in the pathophysiology of this disease.
authors.affiliation: Department of Surgery, Oulu University, Oulu, Finland. minna.forsman@ppshp.fi
authors.forename: Minna
authors.initials: M
authors.lastname: Forsman
authors.forename: Virve
authors.initials: V
authors.lastname: Pääkkönen
authors.forename: Leo
authors.initials: L
authors.lastname: Tjäderhane
authors.forename: Jussi
authors.initials: J
authors.lastname: Vuoristo
authors.forename: Leena
authors.initials: L
authors.lastname: Kallioinen
authors.forename: Tuula
authors.initials: T
authors.lastname: Salo
authors.forename: Matti
authors.initials: M
authors.lastname: Kallioinen
authors.forename: Jorma
authors.initials: J
authors.lastname: Ryhänen
mesh_terms: D000328:Adult; D000368:Aged; D026481:Blotting, Far-Western; D004387:Dupuytren Contracture / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D020022:Genetic Predisposition to Disease; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009211:Myoglobin / Q000096:biosynthesis*; D057050:Receptor Tyrosine Kinase-like Orphan Receptors; D011956:Receptors, Cell Surface / Q000096:biosynthesis*
chemical_list: D009211:Myoglobin; D011956:Receptors, Cell Surface; C078717:ROR2 protein, human; D057050:Receptor Tyrosine Kinase-like Orphan Receptors