Gene expression profile related to prognosis of acute myeloid leukemia.
Acute myeloid leukemia (AML) is a heterogeneous group of diseases with respect to biology and clinical course. Through genome-wide scanning, we can have an improvement of the diagnosis and assay system of AML. Microarray was performed for the identification of acute myeloid leukemia prognosis. We divided patients into two groups (good prognosis group, GPG and poor prognosis group, PPG) based on differences in the individual reactions to treatment. Gene expression profiles were analyzed using microarray. Among genes up-regulated at least two-fold and down-regulated at least 0.5-fold in HL-60, we chose three up-regulated genes (PPP2CA, ME3, and CCDN2) and three down-regulated genes (GLO1, ANXA2, and BMI1) and confirmed the expression of these six genes by RT-PCR. We created a leukemia-specific subclass microarray, based on the gene expression profiles. Clinical samples from the bone marrow of four patients were hybridized on this microarray. Among the genes selected by the microarray technology, NB4, silenced TRIB3 and overexpressed XRN2 were not differentiated in spite of treatment with ATRA. This indicates that XRN2 and TRIB3 play an important role in cell differentiation. These data provided an expression profile for the diagnosis and prognosis of AML patients and identified candidate genes that might allow the prognosis of AML through the relative comparison of the expression level of genes between GPG and PPG.
Park
Min Ha
MH
Biological Science, Sookmyung Women's University, Seoul 140-742, Korea.
Cho
Sun A
SA
Yoo
Kyung Hyun
KH
Yang
Moon Hee
MH
Ahn
Ji Young
JY
Lee
Hyo Soo
HS
Lee
Kyoung-Eun
KE
Mun
Yeung-Chul
YC
Cho
Dae Ho
DH
Seong
Chu Myong
CM
Park
Jong Hoon
JH
D017306:Annexin A2 / Q000235:genetics; D045744:Cell Line, Tumor; D015536:Down-Regulation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D018922:HL-60 Cells; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D054648:Protein Phosphatase 2 / Q000235:genetics; D015854:Up-Regulation
C472822:ANXA2 protein, human; D017306:Annexin A2; C517189:PPP2CA protein, human; D054648:Protein Phosphatase 2