[Expression of K18, Ser-33 and Ser-52 phosphorylated K18 in HBV infected human liver disease and its significance].
OBJECTIVE
To investigate the expression of K18, Ser-33 and Ser-52 phosphorylated K18 in HBV infected human liver disease and its significance.


METHODS
The expression and localization of K18 and Ser-33, Ser-52 phosphorylated K18 in healthy liver tissue, in liver tissues of patients with post-HBV infection cirrhosis and severe chronic hepatitis were detected by histochemistry.


RESULTS
K18, Ser-33 and Ser-52 phosphorylated K18 were expressed in normal liver cells, in liver tissues of cirrhosis patients and severe chronic hepatitis cases. The expression of K18 in the liver cells from the 3 different sources had no significant difference in levels. Ser-33 and Ser-52 phosphorylated K18 were expressed in normal liver cells, in liver tissues of cirrhosis patients chronicity HBV hepatitis and severe chronic hepatitis cases. Ser-33 and Ser-52 located around cytoplasmic membrane, diffused into cytoplasm and expressed at a higher levels in cirrhosis and severe chronic hepatitis.


CONCLUSIONS
The expression levels of Ser-33 and Ser-52 phosphorylated K18 increased along with the progression of HBV infected human liver disease. The phosphorylation of K18 could be a marker of progression of HBV infected human liver disease.
Shi
Ying
Y
Department of Infectious Diseases, Beijing Youan Hospital, Capital University of Medical Sciences, Beijing, China.
Li
Juan
J
Liang
Lian-Chun
LC
Wu
Ya-Song
YS
Zhou
Yu-Sen
YS
Chen
Xin-Yue
XY
Chen
De-Xi
DX
Wu
Hao
H
D006509:Hepatitis B / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D053538:Keratin-18 / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000378:metabolism / Q000473:pathology / Q000821:virology; D008107:Liver Diseases / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D010766:Phosphorylation; D012694:Serine / Q000378:metabolism
D053538:Keratin-18; D012694:Serine