title: [Influence of artificial pneumoperitoneal media on colon carcinoma cell proliferation in vitro].
abstract: OBJECTIVE
  To evaluate the influence of different pneumoperitoneal media on colon carcinoma LS-174T cell proliferation in vitro.
  
  
  METHODS
  The artificial pneumoperitoneum was established. The proliferation of LS-174T cells was detected by MTT assay and soft agar clone formation assay. Expression of HIF-1alpha and VEGF was examined by immunohistochemistry. Apoptosis of LS-174T cells was analyzed by AO/EB double fluorescein stain and flow cytometry.
  
  
  RESULTS
  The growth speed and proliferating capacity of LS-174T cells in CO(2) pneumoperitoneum group[A:0.37 +/- 0.02,formation (32.8 +/- 3.6)%] were significantly higher than those in control group [A:0.33 +/- 0.01,formation (28.4 +/- 2.3)%] and He group [A:0.30 +/- 0.01,formation (23.5 +/- 2.7)%], meanwhile the He group was the lowest (P<0.01). Positive expression of HIF-1alpha and VEGF in CO(2) and He artificial pneumoperitoneum up-regulated significantly as compared to control group(P<0.01), meanwhile the above expression was higher in CO(2) group (P<0.01). The G(0 )/G(1) ratio in CO(2) group was the lowest as compared to control group and He group (P<0.01), and G(0 )/G(1) ratio in He group was higher than that of control group(P<0.01). Aapoptosis rate in He group was the highest as compared with the other two groups(P<0.01).
  
  
  CONCLUSIONS
  CO(2) pneumoperitoneum has stronger effect on the proliferation of colon carcinoma cell LS-174T as compared to He pneumoperitoneum in vitro.
authors.affiliation: Department of ICU, The Second Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Tian
authors.forename: Yin-Hua
authors.initials: YH
authors.lastname: Tang
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Dai
authors.forename: Su
authors.initials: S
authors.lastname: Liu
authors.forename: Jin-Peng
authors.initials: JP
authors.lastname: Zhao
authors.forename: Yong
authors.initials: Y
authors.lastname: Ma
authors.forename: Hong-Chi
authors.initials: HC
authors.lastname: Jiang
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D005434:Flow Cytometry; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D011028:Pneumoperitoneum, Artificial / Q000379:methods*
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit