title: Postmortem biochemical behavior of giant squid (Dosidicus gigas) mantle muscle stored in ice and its relation with quality parameters.
abstract: Several freshness and spoilage indicators were monitored to characterize the postmortem biochemistry of giant squid (Dosidicus gigas) mantle muscle. Squid samples were obtained directly from the sea and kept at 0 degrees C during a 15-d storage period. Data at zero time were obtained from cryogenically frozen samples at time of capture. The adenosine 5'-triphosphate (ATP) degradation followed a different pattern as compared with that from fish species. ATP was almost completely depleted at 24-h postcatch from 6.54 to <1 micromol/g, while at the same time Hx was the predominant catabolite with a concentration of 4 mumol/g, reaching 6.85 micromol/g at day 15. K-value data followed a logarithmic pattern with time instead of a linear one, with no change after day 3, thus reducing its suitability as a freshness index. The coefficient Hx/AMP seems to be an adequate alternative for this purpose due to its constant increment with time. The high NH4Cl content in mantle muscle (461.3 +/- 24.5 mg of NH4(+)/100 g) derived from its physiological importance for the species compromises the use of the distillation step of the TVB-N analysis commonly used as a spoilage index. This fact explains why the initially high value of TVB-N detected in mantle muscle (243.7 mg N/100 g) did not correlate with the initial low TMA-N content (1.5 +/- 0.1 mg/100 g of muscle). The results suggested that under the experimental conditions the shelf life of squid exceeds 15 d.
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. P.O. Box 1735. C.P. 83000, Hermosillo, Sonora, México.
authors.forename: E
authors.initials: E
authors.lastname: Márquez-Ríos
authors.forename: E F
authors.initials: EF
authors.lastname: Morán-Palacio
authors.forename: M E
authors.initials: ME
authors.lastname: Lugo-Sánchez
authors.forename: V M
authors.initials: VM
authors.lastname: Ocano-Higuera
authors.forename: R
authors.initials: R
authors.lastname: Pacheco-Aguilar
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D003258:Consumer Behavior; D003257:Consumer Product Safety; D015925:Cryopreservation / Q000379:methods*; D049832:Decapodiformes / Q000737:chemistry* / Q000378:metabolism; D005519:Food Preservation / Q000379:methods*; D006801:Humans; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism; D011180:Postmortem Changes; D012758:Shellfish / Q000032:analysis / Q000592:standards*; D013045:Species Specificity; D013997:Time Factors
chemical_list: D000255:Adenosine Triphosphate