title: Quantifying small numbers of antibodies with a 'near-universal' protein-DNA chimera.
abstract: We present general means to greatly increase the sensitivity of antibody-based assays. Augmentation relies on a 'tadpole' protein-DNA chimera whose protein moiety binds most classes of mammalian antibodies but not avian immunoglobulin Y (IgY). We used this tadpole in affinity capture assays followed by real-time PCR to quantify numerous molecules, including prostate-specific antigen (PSA) in human serum, with great sensitivity and accuracy.
authors.affiliation: Center for Quantitative Genome Function and Molecular Sciences Institute, 2168 Shattuck Avenue, Berkeley, California 94704, USA.
authors.forename: Ian
authors.initials: I
authors.lastname: Burbulis
authors.forename: Kumiko
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Richard
authors.initials: R
authors.lastname: Yu
authors.forename: Orna
authors.initials: O
authors.lastname: Resnekov
authors.forename: Roger
authors.initials: R
authors.lastname: Brent
mesh_terms: D004247:DNA / Q000276:immunology; D004268:DNA-Binding Proteins / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008832:Microchemistry / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D004247:DNA; D017430:Prostate-Specific Antigen