[Clone of HPV58 E6 gene and analysis of HLA-DQB1 03 restricted T-cell epitopes].
OBJECTIVE
To clone HPV58 E6 gene and analyze the HLA-DQB1 03-restricted T cell epitopes on E6 protein.


METHODS
Total genome DNA was isolated from a cervical cancer sample. The HPV58 E6 gene was amplified by PCR and inserted into pGEM-T Easy vector. The recombinant plasmid was identified by restriction endonuclease analysis and sequencing. HLA-DQB1 03-restricted T cell epitopes on E6 protein were predicted and analyzed by the position-specific scoring matrix, support vector machine theory and prediction algorithm for proteasomal cleavages.


RESULTS
A HPV58 E6 gene was successfully cloned and submitted to GenBank (EF060239). Epitope 47(FADLRIAYRDGNPFA) and Epitope 102(RCIICQRPLCPQEKK) were theoretic HLA-DQB1 03-restricted T cell epitopes on E6 protein.


CONCLUSIONS
These two epitopes could serve as candidates for screening and identification of the vaccine against HPV58 infection in the further study.
Chen
Ling
L
Department of Dermatology, Institute of Battle Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Shen
Zhu
Z
Wu
Jin-Jin
JJ
Zhang
Ju
J
Zhou
Yang
Y
Fan
Xue-Li
XL
D000328:Adult; D052685:Alphapapillomavirus / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D001483:Base Sequence; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D018984:Epitopes, T-Lymphocyte / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*; D005260:Female; D006683:HLA-DQ Antigens / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D053918:Papillomavirus Vaccines / Q000032:analysis / Q000276:immunology; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D016415:Sequence Alignment; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
D018984:Epitopes, T-Lymphocyte; D006683:HLA-DQ Antigens; D053918:Papillomavirus Vaccines; D014764:Viral Proteins; D046988:Proteasome Endopeptidase Complex