title: [Mesenchymal stem cells inhibit the expression of CD25 on phytohaemagglutinin-activated lymphocytes].
abstract: OBJECTIVE
  To investigate the regulatory effects of rat mesenchymal stem cell (MSC) on T lymphocyte proliferation by examining the early activated markers such as CD25 and CD69.
  
  
  METHODS
  MSC had been isolated and expanded in vitro. Then it was identified by cell morphology, membrane phenotype, and differentiation potential. Nylon wool column was applied to purify T-lymphocytes. MSCs and T-lymphocytes were cultured together and were stimulated by phytohaemagglutinin (PHA), and then the expressions of CD25 and CD69 were assessed. The levels of TGF-beta1 and IL-10 in the supernatants of MSC cultures were detected by using ELISA.
  
  
  RESULTS
  (1) The expression of CD25 is suppressed in a dose-dependent manner when the T-lymphocytes are co-cultured with 10,000 MSCs or more, while 100 MSCs have no detectable effect; (2) The suppression of CD25 can be lasted more than 96 hrs; (3) The down regulation of CD25 is mediated by some soluble factors; (4) The reduced expression of CD25 caused by MSC inhibition is not mediated by TGF-beta1 and IL-10.
  
  
  CONCLUSIONS
  MSCs have significant immune regulatory effects on PHA-stimulated T-lymphocyte culture. It might provide a remarkable immune suppression in organ-transplantation to achieve better outcome in the near future.
authors.affiliation: Department of General Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
authors.forename: Ying-bin
authors.initials: YB
authors.lastname: Liu
authors.forename: Hao-ran
authors.initials: HR
authors.lastname: Qian
authors.forename: De-fei
authors.initials: DF
authors.lastname: Hong
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Wang
authors.forename: Jiang-tao
authors.initials: JT
authors.lastname: Li
authors.forename: Xu-an
authors.initials: XA
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Kun
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: De-qin
authors.initials: DQ
authors.lastname: Chen
authors.forename: Wei-hong
authors.initials: WH
authors.lastname: Weng
authors.forename: Shu-you
authors.initials: SY
authors.lastname: Peng
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis; D002478:Cells, Cultured; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000737:chemistry; D005434:Flow Cytometry; D016753:Interleukin-10 / Q000032:analysis; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis*; D037181:Lectins, C-Type; D008213:Lymphocyte Activation / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D023461:Myeloid Progenitor Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000032:analysis; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017077:Culture Media, Conditioned; D016753:Interleukin-10; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D010835:Phytohemagglutinins; D053773:Transforming Growth Factor beta1; D000099192:CD69 Antigens