title: Ex-vivo NMR of unprocessed tissue in water: a simplified procedure for studying intracranial neoplasms.
abstract: Ex-vivo and in-vitro nuclear magnetic resonance (NMR) spectroscopy techniques have been used for studying chemical metabolites in surgically resected specimens of human neoplasms, and may provide complementary information to in-vivo whole-body magnetic-resonance spectroscopy (MRS). We describe an ex-vivo NMR in water method for measurement of water-soluble metabolites in unprocessed normal rat brain tissue and human intracranial neoplasms. The NMR spectra obtained using the method described here were comparable to those obtained using high-resolution magic-angle spinning (HRMAS) NMR methods, with good correlation in metabolite concentrations relative to creatine (r2 = 0.7635). Improved spectral resolution and baseline were noted compared to HRMAS, but macromolecule resonances were not detected. Ex-vivo NMR of unprocessed tissue in water is rapid and technically simple to perform, and has the potential to be used for direct assessment of intracranial neoplasms.
authors.affiliation: Department of Neuroradiology, National Neuroscience Institute, 11 Jalan Tan Tock Seng, Singapore, 308433, Singapore.
authors.forename: Maosheng
authors.initials: M
authors.lastname: Xu
authors.forename: Jieru
authors.initials: J
authors.lastname: Ye
authors.forename: Daiwen
authors.initials: D
authors.lastname: Yang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xu
authors.forename: Tseng Tsai
authors.initials: TT
authors.lastname: Yeo
authors.forename: Wai Hoe
authors.initials: WH
authors.lastname: Ng
authors.forename: C C
authors.initials: CC
authors.lastname: Lim
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D003404:Creatinine / Q000032:analysis / Q000378:metabolism*; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D009837:Oligodendroglioma / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014867:Water / Q000378:metabolism
chemical_list: D014867:Water; D003404:Creatinine