Chryseobacterium hominis sp. nov., to accommodate clinical isolates biochemically similar to CDC groups II-h and II-c.
A collection of eight clinical strains from Belgian hospitals and three clinical strains of the CCUG collection were characterized biochemically as being similar to CDC groups II-h and II-c; the latter differs from group II-h only by positivity for sucrose acidification. These 11 strains were found to cluster according to 16S rRNA gene sequence similarity at a level of >or=99.5%, and on the basis of their tDNA-PCR profile. Based on 16S rRNA gene sequence analysis, this collection of strains was related most closely to Chryseobacterium hispanicum (97.2%), but they differed from the type strain of this species by the following phenotypic characteristics: growth at 37 degrees C, negativity for xylose acidification, positivity for acetate assimilation-alkalinization on Simmons' agar base and absence of flexirubin pigments, and by their tDNA-PCR profile. Strain NF802T showed only 57.8% DNA-DNA relatedness to the type strain of C. hispanicum. Fatty acid composition did not enable differentiation from C. hispanicum. The DNA G+C content of strain NF802T is 36.5 mol%. The name Chryseobacterium hominis sp. nov. is proposed for this taxon, with type strain NF802T (=CCUG 52711T=CIP 109415T).
Vaneechoutte
Mario
M
Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium.
Kämpfer
Peter
P
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Germany.
De Baere
Thierry
T
Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium.
Avesani
Véronique
V
Microbiology Unit, Faculty of Medicine, University of Louvain, Brussels, Belgium.
Janssens
Michèle
M
Microbiology Unit, Faculty of Medicine, University of Louvain, Brussels, Belgium.
Wauters
Georges
G
Microbiology Unit, Faculty of Medicine, University of Louvain, Brussels, Belgium.
D015373:Bacterial Typing Techniques; D002487:Centers for Disease Control and Prevention, U.S.; D045247:Chryseobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D045826:Flavobacteriaceae Infections / Q000382:microbiology*; D020459:Genes, rRNA; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA; D014481:United States
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012343:RNA, Transfer