PrP N-terminal domain triggers PrP(Sc)-like aggregation of Dpl.
Transmissible spongiform encephalopathies are fatal neurodegenerative disorders thought to be transmitted by self-perpetuating conformational conversion of a neuronal membrane glycoprotein (PrP(C), for "cellular prion protein") into an abnormal state (PrP(Sc), for "scrapie prion protein"). Doppel (Dpl) is a protein that shares significant biochemical and structural homology with PrP(C). In contrast to its homologue PrP(C), Dpl is unable to participate in prion disease progression or to achieve an abnormal PrP(Sc)-like state. We have constructed a chimeric mouse protein, composed of the N-terminal domain of PrP(C) (residues 23-125) and the C-terminal part of Dpl (residues 58-157). This chimeric protein displays PrP-like biochemical and structural features; when incubated in presence of NaCl, the alpha-helical monomer forms soluble beta-sheet-rich oligomers which acquire partial resistance to pepsin proteolysis in vitro, as do PrP oligomers. Moreover, the presence of aggregates akin to protofibrils is observed in soluble oligomeric species by electron microscopy.
Erlich
Paul
P
Laboratoire Adaptation et Pathogénie des Micro-organismes, Université Joseph Fourier, BP 170, 38042 Grenoble Cedex 9, France.
Cesbron
Jean-Yves
JY
Lemaire-Vieille
Catherine
C
Curt
Aurélie
A
Andrieu
Jean-Pierre
JP
Schoehn
Guy
G
Jamin
Marc
M
Gagnon
Jean
J
D000818:Animals; D058851:GPI-Linked Proteins; D051379:Mice; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018621:PrPC Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011328:Prions / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000737:chemistry; D012996:Solutions
D058851:GPI-Linked Proteins; D010446:Peptide Fragments; D018621:PrPC Proteins; D011328:Prions; C122036:Prnd protein, mouse; D011993:Recombinant Fusion Proteins; D012996:Solutions; D012965:Sodium Chloride