The K/BxN mouse model of inflammatory arthritis: theory and practice.
Mice expressing the KRN T cell receptor transgene and the MHC class II molecule A(g7) (K/BxN mice) develop severe inflammatory arthritis, and serum from these mice causes similar arthritis in a wide range of mouse strains, owing to pathogenic autoantibodies to glucose-6-phosphate isomerase (GPI). This model has been useful for the investigation of the development of autoimmunity (K/BxN transgenic mice) and particularly of the mechanisms by which anti-GPI autoantibodies induce joint-specific imflammation (serum transfer model). In this chaper, after a summary of findings from this model system, we describe detailed methods for the maintenance of a K/BxN colony, crossing of the relevant TCR and MHC genes to other strain backgrounds, evaluation of KRN transgenic T cells, measurement of anti-GPI antibodies, induction of arthritis by serum transfer, and clinical and histological evaluation of arthritis.
Monach
Paul
P
Section of Immunology and Immunogenetics, Joslin Diabetes Center, Boston, MA, USA.
Hattori
Kimie
K
Huang
Haochu
H
Hyatt
Elzbieta
E
Morse
Jody
J
Nguyen
Linh
L
Ortiz-Lopez
Adriana
A
Wu
Hsin-Jung
HJ
Mathis
Diane
D
Benoist
Christophe
C
D000818:Animals; D000843:Ankle Joint / Q000473:pathology; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D005802:Genes, MHC Class II; D005838:Genotype; D005956:Glucose-6-Phosphate Isomerase / Q000276:immunology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology
D001323:Autoantibodies; D011948:Receptors, Antigen, T-Cell; D005956:Glucose-6-Phosphate Isomerase