Nano-particle labelling of nucleic acids for enhanced detection by inductively-coupled plasma mass spectrometry (ICP-MS).
Oligonucleotides containing a biotin functionality were successfully labelled with a streptavidin nanogold conjugate and subsequently separated and analysed by high performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS).
Kerr
Samantha Louise
SL
Department of Chemistry, Loughborough University, Loughborough, Leicestershire, UK LE11 3TU.
Sharp
Barry
B
D001710:Biotin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000032:analysis / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry*; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*
D009841:Oligonucleotides; D001710:Biotin; D006046:Gold; D004247:DNA; D019809:Streptavidin