In situ synthesis of peptide microarrays using ink-jet microdispensing.
The study of protein-protein and protein-DNA interactions is critical to understand biological processes. This article presents the methodology to create peptide microarrays in situ for the high-throughput screening of complex biomolecules. The in situ ink-jet peptide synthesis results in a conservation of costly reagent and amino acids, whereas providing a means to produce denser peptide arrays. A smaller amount of test sample is required to observe interaction when using these high-density peptide arrays.
Antohe
Bogdan V
BV
MicroFab Technologies, Inc., Plano, TX, USA.
Cooley
Patrick W
PW
D000818:Animals; D000906:Antibodies; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D020650:Combinatorial Chemistry Techniques / Q000295:instrumentation* / Q000379:methods*; D004745:Enkephalins / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008970:Molecular Weight; D040081:Protein Array Analysis / Q000379:methods*
D000906:Antibodies; D004745:Enkephalins