Fascin expression in human embryonic, fetal, and normal adult tissue.
This study investigates the distribution of fascin in human embryonic, fetal, and normal adult tissues. Tissue microarray technology was used to perform immunohistochemical experiments on human embryos and fetuses at 4-22 weeks of gestation and adult specimens. Fascin was widely expressed in the nervous system. At 4 weeks of gestation, fascin was present in the neural tube. At 8-12 weeks of gestation, homogenous gene expression was seen in cells of the cerebellum and gastrointestinal tract. In later developmental stages and in adults, Purkinje cells of the cerebellum and glandular epithelium of the gastrointestinal tract showed no expression. Fascin was expressed in the cortex and medulla of the adrenal gland at 8-12 weeks of gestation, whereas immunoreactivity decreased from the zona glomerulosa through the zona reticularis and was essentially negative in the adrenal medulla of adults. Significant expression of fascin was seen throughout development in neurons, follicular dendritic cells of lymphoid tissue, basal layer cells of stratified squamous epithelia, mesenchyme, and vascular endothelial cells. Simple columnar epithelia of the biliary duct, colon, ovary, pancreas, and stomach were all negative for fascin expression. These results show that expression of fascin is time specific and highly tissue specific. Parallels between fascin expression in embryogenesis and carcinogenesis are discussed.
Zhang
Fa-Ren
FR
Department of Biochemistry and Molecular Biology, Medical College of Shantou University, 22 Xinling Road, Shantou City 515041, Guangdong Province, China.
Tao
Li-Hua
LH
Shen
Zhong-Ying
ZY
Lv
Zhuo
Z
Xu
Li-Yan
LY
Li
En-Min
EM
D000293:Adolescent; D000328:Adult; D002319:Cardiovascular System / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis*; D004622:Embryo, Mammalian / Q000378:metabolism*; D004703:Endocrine System / Q000378:metabolism; D005333:Fetus / Q000378:metabolism*; D041981:Gastrointestinal Tract / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007231:Infant, Newborn; D008840:Microfilament Proteins / Q000096:biosynthesis*; D008875:Middle Aged; D009420:Nervous System / Q000378:metabolism; D009928:Organ Specificity; D046888:Tissue Array Analysis
D002352:Carrier Proteins; D008840:Microfilament Proteins; C020324:fascin