OPN/CD44v6 overexpression in laryngeal dysplasia and correlation with clinical outcome.
Laryngeal dysplasia is a common clinical concern. Despite major advancements, a significant number of patients with this condition progress to invasive squamous cell carcinoma. Osteopontin (OPN) is a secreted glycoprotein, whose expression is markedly elevated in several types of cancers. We explored OPN as a candidate biomarker for laryngeal dysplasia. To this aim, we examined OPN expression in 82 cases of dysplasia and in hyperplastic and normal tissue samples. OPN expression was elevated in all severe dysplasia samples, but not hyperplastic samples, with respect to matched normal mucosa. OPN expression levels correlated positively with degree of dysplasia (P=0.0094) and negatively with disease-free survival (P<0.0001). OPN expression was paralleled by cell surface reactivity for CD44v6, an OPN functional receptor. CD44v6 expression correlated negatively with disease-free survival, as well (P=0.0007). Taken as a whole, our finding identify OPN and CD44v6 as predictive markers of recurrence or aggressiveness in laryngeal intraepithelial neoplasia, and overall, point out an important signalling complex in the evolution of laryngeal dysplasia.
Staibano
S
S
Dipartimento di Scienze Biomorfologiche e Funzionali, Università di Napoli Federico II, Naples, Italy.
Merolla
F
F
Testa
D
D
Iovine
R
R
Mascolo
M
M
Guarino
V
V
Castellone
M D
MD
Di Benedetto
M
M
Galli
V
V
Motta
S
S
Melillo
R M
RM
De Rosa
G
G
Santoro
M
M
Celetti
A
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis*; D007150:Immunohistochemistry; D007818:Laryngeal Diseases / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D053495:Osteopontin / Q000032:analysis*; D010610:Pharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology; D011379:Prognosis
C112697:CD44v6 antigen; D006023:Glycoproteins; D018960:Hyaluronan Receptors; D053495:Osteopontin