Platelet gel stimulates proliferation of human dermal fibroblasts in vitro.
BACKGROUND
Platelet gel is a source of many soluble proteins that can modulate tissue regeneration. Dermal fibroblasts play an important role in tissue remodeling and wound healing.


OBJECTIVE
The study was performed to investigate the influence of platelet gel and its active ingredients on the proliferation of human dermal fibroblasts in vitro.


METHODS
A fibroblast culture was established from a normal human skin biopsy. Platelet gel was prepared from platelet-rich plasma (PRP) following the addition of calcified thrombin solution. Cell proliferation was measured by MTT assay.


RESULTS
We showed that platelet gel, PRP, and thrombin stimulate the proliferation of dermal fibroblasts, and that stimulation by PRP is dose dependent.


CONCLUSIONS
Platelet gel can be used to treat chronic skin defects.
Krasna
M
M
Department for Blood Supply, Blood Transfusion Center of Slovenia, Slajmerjeva 6, 1000 Ljubljana, Slovenia. metka.krasna@ztm.si
Domanović
D
D
Tomsic
A
A
Svajger
U
U
Jeras
M
M
D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D005782:Gels; D006801:Humans; D053657:Platelet-Rich Plasma; D013917:Thrombin / Q000494:pharmacology*
D005782:Gels; D013917:Thrombin