[Identification and clinical evaluation of lung cancer serum biomarker L-lactate dehydrogenase B].
OBJECTIVE
To identify potential biomarkers related with lung cancer metastasis.


METHODS
Conditional media proteins collected from a primary non-small cell lung cancer cell line (NSCLC) NCI-H226 and its brain metastatic subline H226Br were analyzed by SDS-PAGE and MALDI-TOF-MS. Twelve biomarkers were identified, of which LDHB was significantly up-regulated in H226Br cell and was further validated using ELISA in sera including 105 lung cancer samples, 41 pneumonia and pulmonary tuberculosis samples and 65 healthy samples.


RESULTS
The levels of LDHB were specifically elevated in NSCLC sera [A value 0.485 (0 - 1.415)] compared with pneumonia and pulmonary tuberculosis [A value 0.187 (0 - 0.609), P < 0.01] and healthy group [A value, 0.159 (0 - 0.524), P < 0.01] and were progressively increased with the clinical stage. At the cutoff point 0.260 (A value) on the ROC curve, the sensitivity, specificity and total accuracy of LDHB were 81%, 70% and 76% respectively.


CONCLUSIONS
These findings demonstrated that secretome could open up a possibility to identify novel biomarkers related with cancer occurrence and metastasis.
Chen
Yue
Y
Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, Beijing Normal University, Beijing 100875, China.
Zhang
Hao
H
Xu
An-jian
AJ
Liu
Ji-fu
JF
Li
Na
N
Wu
Shan-shan
SS
Huang
Ling-yun
LY
He
Da-cheng
DC
Xiao
Xue-yuan
XY
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood* / Q000378:metabolism; D008175:Lung Neoplasms / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D040901:Proteomics / Q000379:methods; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000378:metabolism / Q000473:pathology
D014408:Biomarkers, Tumor; D017077:Culture Media, Conditioned; D007770:L-Lactate Dehydrogenase