Canine model of ischemic stroke with permanent middle cerebral artery occlusion: clinical and histopathological findings.
The aim of the present study was to assess the clinical and histopathological findings in a canine model of ischemic stroke. Cerebral ischemic stroke was induced by middle cerebral artery occlusion in four healthy beagle dogs using silicone plugs. They showed neurological signs of forebrain dysfunction such as reduced responsiveness, head turning, circling, postural reaction deficits, perceptual deficits, and hemianopsia. These signs gradually regressed within 4 weeks without therapy. On magnetic resonance imaging, T2 hyperintensity and T1 hypointensity were found in the cerebral cortex and basal ganglia. These lesions were well-defined and sharply demarcated from adjacent brain parenchyma with a homogenous appearance. No abnormalities of the cerebrospinal fluid were observed. At necropsy, atrophic and necrotic lesions were observed in the cerebral cortex. The cerebral cortex, basal ganglia, and thalamus were partially unstained with triphenyl- tetrazolium chloride. Histopathologically, typical features of infarction were identified in cortical and thalamic lesions. This study demonstrates that our canine model resembles the conditions of real stroke patients.
Kang
Byeong-Teck
BT
Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea.
Lee
Jong-Hwan
JH
Jung
Dong-In
DI
Park
Chul
C
Gu
Su-Hyun
SH
Jeon
Hyo-Won
HW
Jang
Dong-Pyo
DP
Lim
Chae-Young
CY
Quan
Fu-Shi
FS
Kim
Young-Bo
YB
Cho
Zang-Hee
ZH
Woo
Eung-Je
EJ
Park
Hee-Myung
HM
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002544:Cerebral Infarction / Q000209:etiology* / Q000473:pathology*; D002555:Cerebrospinal Fluid / Q000737:chemistry / Q000166:cytology; D004195:Disease Models, Animal; D004285:Dogs; D020244:Infarction, Middle Cerebral Artery / Q000150:complications* / Q000473:pathology*; D008279:Magnetic Resonance Imaging; D008297:Male