Duplication and segregation of the actin (MreB) cytoskeleton during the prokaryotic cell cycle.
The bacterial actin homolog MreB exists as a single-copy helical cytoskeletal structure that extends between the two poles of rod-shaped bacteria. In this study, we show that equipartition of the MreB cytoskeleton into daughter cells is accomplished by division and segregation of the helical MreB array into two equivalent structures located in opposite halves of the predivisional cell. This process ensures that each daughter cell inherits one copy of the MreB cytoskeleton. The process is triggered by the membrane association of the FtsZ cell division protein. The cytoskeletal division and segregation events occur before and independently of cytokinesis and involve specialized MreB structures that appear to be intermediates in this process.
Vats
Purva
P
Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030, USA.
Rothfield
Lawrence
L
D002453:Cell Cycle; D002455:Cell Division; D003599:Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure; D004926:Escherichia coli / Q000166:cytology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D011487:Protein Conformation
D029968:Escherichia coli Proteins; C078473:MreB protein, E coli