[Full-length cloning of a novel gene, ELF2C, related to familial acute myelogenous leukemia].
OBJECTIVE
To clone the full-length cDNA of a novel gene of familial acute myelogenous leukemia and to explain the molecular mechanism of the disease at the gene level.


METHODS
Based on a EST sequence (zywb4) from a subtractive cDNA library of specially or differentially expressed genes constructed in familial acute myelogenous leukemia, electronic cDNA cloning and SMART-rapid amplification of cDNA ends (SMART-RACE) were used to clone the full-length cDNA of a novel associated gene of familial acute myelogenous leukemia.


RESULTS
One sequence of 2257 bp was obtained, which obeyed Kozak rules, and contained an open reading frame (ORF) and a polyA tail. BLAST analysis showed that it may be a novel gene. The new sequence was submitted to GenBank with the accession number: DQ359746 and designated as ELF2C.


CONCLUSIONS
A full- length cDNA of a novel gene (ELF2C) related to familial acute myelogenous leukemia is obtained, which is helpful to further investigation of its function in familial acute myelogenous leukemia.
Wang
Cheng-Yi
CY
Fujian Institute of Hematology, Department of Hematology, Union Hospital, Fujian Medical University, Fuzhou 350001, China.
Wang
Shao-Yuan
SY
Lin
Xu
X
Zhang
Yi-Wen
YW
Li
Jing-Gang
JG
D002648:Child; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015723:Gene Library; D020022:Genetic Predisposition to Disease; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics*
D018076:DNA, Complementary; D014157:Transcription Factors; C101003:ELF2 protein, human