title: B-cell chronic lymphocytic leukaemia cells show specific changes in membrane protein expression during different stages of cell cycle.
abstract: The proliferating component in chronic lymphocytic leukaemia (CLL) is usually small (<1%) and restricted to a specific micro-environmental niche. To characterize the proliferating component, CLL cells from bone marrow or lymph nodes of 23 patients were assessed for expression of up to 66 surface antigens in combination with nuclear Ki-67/MCM6. Ki-67 expression was associated with step-wise increases in CD23/CD95/CD86/CD39/CD27 and decreases in CD24/CD69/CXCR4/CXCR5. Ki-67+ cells showed increased CD38 expression, but with considerable inter-patient variability: in some cases Ki-67 expression was only detectable in CD38- CLL cells. The results suggest continuous re-entry into the cell cycle as no distinct stem cell pool was detectable.
authors.affiliation: Haematological Malignancy Diagnostic Service, Leeds Teaching Hospitals, Leeds, UK.
authors.forename: Fiona
authors.initials: F
authors.lastname: Bennett
authors.forename: Andy
authors.initials: A
authors.lastname: Rawstron
authors.forename: Marieth
authors.initials: M
authors.lastname: Plummer
authors.forename: Ruth
authors.initials: R
authors.lastname: de Tute
authors.forename: Paul
authors.initials: P
authors.lastname: Moreton
authors.forename: Andrew
authors.initials: A
authors.lastname: Jack
authors.forename: Peter
authors.initials: P
authors.lastname: Hillmen
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology*; D049109:Cell Proliferation; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D019394:Ki-67 Antigen / Q000378:metabolism; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism* / Q000473:pathology; D008565:Membrane Proteins / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D019394:Ki-67 Antigen; D008565:Membrane Proteins; D019707:Receptors, Chemokine