title: Biochips: non-conventional strategies for biosensing elements immobilization.
abstract: The present article draws a general picture of non-conventional methods for biomolecules immobilization. The technologies presented are based either on original solid supports or on innovative immobilization processes. Polydimethylsiloxane elastomer will be presented as a popular immobilization support within the biochip developer community. Electro-addressing of biomolecules at the surface of conducting biochips will appear to be an interesting alternative to immobilization processes based on surface functionalization. Finally, bead-assisted biomolecules immobilization will be presented as an open field of research for biochip developments.
authors.affiliation: Laboratoire de Genie Enzymatique et Biomoleculaire, UMR 5246 ICBMS, CNRS-Universite Claude Bernard Lyon 1, Bat CPE, 43, bd du 11 novembre 1918, 69622 Villeurbanne, Cedex, France. christophe.marquette@univlyon1.fr
authors.forename: Christophe A
authors.initials: CA
authors.lastname: Marquette
authors.forename: Benjamin P
authors.initials: BP
authors.lastname: Corgier
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Heyries
authors.forename: Loic J
authors.initials: LJ
authors.lastname: Blum
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D004867:Equipment Design; D005821:Genetic Techniques; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D010100:Oxygen / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D012828:Silicones / Q000737:chemistry
chemical_list: D004129:Dimethylpolysiloxanes; D008051:Lipid Bilayers; D008055:Lipids; D011108:Polymers; D012828:Silicones; C013830:baysilon; D006861:Hydrogen Peroxide; D010100:Oxygen