title: JNK1 in hematopoietically derived cells contributes to diet-induced inflammation and insulin resistance without affecting obesity.
abstract: Obesity-induced insulin resistance is a major factor in the etiology of type 2 diabetes, and Jun kinases (JNKs) are key negative regulators of insulin sensitivity in the obese state. Activation of JNKs (mainly JNK1) in insulin target cells results in phosphorylation of insulin receptor substrates (IRSs) at serine and threonine residues that inhibit insulin signaling. JNK1 activation is also required for accumulation of visceral fat. Here we used reciprocal adoptive transfer experiments to determine whether JNK1 in myeloid cells, such as macrophages, also contributes to insulin resistance and central adiposity. Our results show that deletion of Jnk1 in the nonhematopoietic compartment protects mice from high-fat diet (HFD)-induced insulin resistance, in part through decreased adiposity. By contrast, Jnk1 removal from hematopoietic cells has no effect on adiposity but confers protection against HFD-induced insulin resistance by decreasing obesity-induced inflammation.
authors.affiliation: Department of Medicine, University of Fribourg, Chemin du Musée 5, CH-1700 Fribourg, Switzerland.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Solinas
authors.forename: Cristian
authors.initials: C
authors.lastname: Vilcu
authors.forename: Jaap G
authors.initials: JG
authors.lastname: Neels
authors.forename: Gautam K
authors.initials: GK
authors.lastname: Bandyopadhyay
authors.forename: Jun-Li
authors.initials: JL
authors.lastname: Luo
authors.forename: Willscott
authors.initials: W
authors.lastname: Naugler
authors.forename: Sergei
authors.initials: S
authors.lastname: Grivennikov
authors.forename: Anthony
authors.initials: A
authors.lastname: Wynshaw-Boris
authors.forename: Miriam
authors.initials: M
authors.lastname: Scadeng
authors.forename: Jerrold M
authors.initials: JM
authors.lastname: Olefsky
authors.forename: Michael
authors.initials: M
authors.lastname: Karin
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage; D004734:Energy Metabolism; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000201:enzymology / Q000378:metabolism*; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D007333:Insulin Resistance; D015850:Interleukin-6 / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008954:Models, Biological; D009765:Obesity / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology; D010168:Palmitates / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D004041:Dietary Fats; D015850:Interleukin-6; D010168:Palmitates; D014409:Tumor Necrosis Factor-alpha; D048031:JNK Mitogen-Activated Protein Kinases