title: Bodipy-VAD-Fmk, a useful tool to study yeast peptide N-glycanase activity.
abstract: In this paper the development of a fluorescent activity based probe, Bodipy-VAD-Fmk, for visualization of yeast peptide N-glycanase is described. The activity based probe is used to assess the efficacy of known and new chitobiose-based electrophilic traps to bind yeast peptide N-glycanase.
authors.affiliation: Bioorganic Synthesis, Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
authors.forename: Martin D
authors.initials: MD
authors.lastname: Witte
authors.forename: Carlos V
authors.initials: CV
authors.lastname: Descals
authors.forename: Sebastiaan V P
authors.initials: SV
authors.lastname: de Lavoir
authors.forename: Bogdan I
authors.initials: BI
authors.lastname: Florea
authors.forename: Gijsbert A
authors.initials: GA
authors.lastname: van der Marel
authors.forename: Herman S
authors.initials: HS
authors.lastname: Overkleeft
mesh_terms: D000590:Amino Acid Chloromethyl Ketones / Q000737:chemistry / Q000378:metabolism*; D005453:Fluorescence; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D000590:Amino Acid Chloromethyl Ketones; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase