title: A microsensor for the detection of a single pathogenic bacterium using magnetotactic bacteria-based bio-carriers: simulations and preliminary experiments.
abstract: The proposed Magnetotactic Bacteria (MTB) based bio-carrier has the potential to greatly improve pathogenic bacteria detection time, specificity, and sensitivity. Microbeads are attached to the MTB and are modified with a coating of an antibody or phage that is specific to the target pathogenic bacteria. Using magnetic fields, the modified MTB are swept through a solution and the target bacteria present become attached to the microbeads (due to the coating). Then, the MTB are brought to the detection region and the number of pathogenic bacteria is determined. The high swimming speed and controllability of the MTB make this method ideal for the fast detection of small concentrations of specific bacteria. This paper focuses on an impedimetric detection system that will be used to identify if a target bacterium is attached to the microbead. The proposed detection system measures changes in electrical impedance as objects (MTB, microbeads, and pathogenic bacteria) pass through a set of microelectrodes embedded in a microfluidic device. FEM simulation is used to acquire the optimized parameters for the design of such a system. Specifically, factors such as electrode/detection channel geometry, object size and position, which have direct effects on the detection sensitivity for a single bacterium or microparticle, are investigated. Polymer microbeads and the MTB system with an E. coli bacterium are considered to investigate their impedance variations. Furthermore, preliminary experimental data using a microfabricated microfluidic device connected to an impedance analyzer are presented.
authors.affiliation: NanoRobotics Laboratory, Department of Computer Engineering, Ecole Polytechnique de Montréal, Canada. rdenomme@engmail.uwaterloo.ca
authors.forename: Ryan C
authors.initials: RC
authors.lastname: Denomme
authors.forename: Zhao
authors.initials: Z
authors.lastname: Lu
authors.forename: Sylvain
authors.initials: S
authors.lastname: Martel
mesh_terms: D000907:Antibodies, Bacterial / Q000737:chemistry; D001424:Bacterial Infections / Q000175:diagnosis / Q000382:microbiology*; D001435:Bacteriophages / Q000737:chemistry; D003198:Computer Simulation; D017097:Electric Impedance; D004926:Escherichia coli / Q000276:immunology / Q000502:physiology / Q000821:virology; D008280:Magnetics; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008863:Microspheres; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial