title: Anti-tumor activity of arginine deiminase via arginine deprivation in retinoblastoma.
abstract: In spite of recent advances in the treatment of retinoblastoma, chemotherapy is still challenging in high-stage intraocular retinoblastoma or metastatic retinoblastoma. Here, we investigated whether arginine deprivation via arginine deiminase (ADI) could be a new anti-tumor therapy in retinoblastoma cells. Expression of argininosuccinate synthetase (ASS) was detected in human retinoblastoma tissues. Even with a high expression of ASS, ADI effectively inhibited the proliferation of retinoblastoma cells and induced retinoblastoma cell death in a dose-dependent manner. These results indicate that arginine deprivation via ADI could be another treatment option for retinoblastoma due to low ASS activity in retinoblastoma cells.
authors.affiliation: Department of Ophthalmology, College of Medicine, Seoul National University, Seoul 110-744, Korea.
authors.forename: Jeong Hun
authors.initials: JH
authors.lastname: Kim
authors.forename: Jin Hyoung
authors.initials: JH
authors.lastname: Kim
authors.forename: Young Suk
authors.initials: YS
authors.lastname: Yu
authors.forename: Dong Hun
authors.initials: DH
authors.lastname: Kim
authors.forename: Bon-Hong
authors.initials: BH
authors.lastname: Min
authors.forename: Kyu-Won
authors.initials: KW
authors.lastname: Kim
mesh_terms: D000230:Adenocarcinoma; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001120:Arginine / Q000378:metabolism*; D001124:Argininosuccinate Synthase / Q000235:genetics / Q000378:metabolism; D001943:Breast Neoplasms; D002455:Cell Division; D045744:Cell Line, Tumor; D002470:Cell Survival; D015353:Eye Enucleation; D005134:Eye Neoplasms / Q000201:enzymology / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D006867:Hydrolases / Q000627:therapeutic use*; D011994:Recombinant Proteins / Q000627:therapeutic use; D012175:Retinoblastoma / Q000201:enzymology / Q000473:pathology / Q000601:surgery
chemical_list: D000970:Antineoplastic Agents; D011994:Recombinant Proteins; D001120:Arginine; D006867:Hydrolases; C019723:arginine deiminase; D001124:Argininosuccinate Synthase