title: Evaluation of matrix metalloproteinase 2 expression in cervical carcinogenesis using tissue array and integrated optical density for immunoreactivity.
abstract: To correlate matrix metalloproteinase-2 (MMP-2) expression with cervical carcinogenesis, the authors use reverse-transcription polymerase chain reaction to detect MMP-2 mRNA expression in 10 cervical squamous cell carcinoma (SCC), 10 high-grade cervical intraepithelial neoplasia (CIN), and 10 normal tissues. They further detect MMP-2 immunoreactivity of 24 tissue cores in each SCC, high-and low-grade CINs, and a healthy subgroup on a tissue array using integrated optical density (IOD) for number and intensity of stained cells in 345 x 345 pixels. They found the mRNA expression of MMP-2 to be higher in most SCCs (9/10 samples) and high-grade CINs (7/10 samples) but lower in normal tissues. The IOD of MMP-2 was significantly higher in high-grade CIN than in normal and low-grade CIN tissue cores (P < .001 for both) and significantly higher in SCC than in high-grade CIN tissue cores (P < .001). The results show that MMP-2 upregulation confers on tumor cells the ability to degrade the subepithelial basement membrane and subsequently invade the cervix.
authors.affiliation: Department of Obstetrics and Gynecology, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Yi-Torng
authors.initials: YT
authors.lastname: Tee
authors.forename: Chih-Ping
authors.initials: CP
authors.lastname: Han
authors.forename: Jiunn-Liang
authors.initials: JL
authors.lastname: Ko
authors.forename: Gin-Den
authors.initials: GD
authors.lastname: Chen
authors.forename: Shun-Fa
authors.initials: SF
authors.lastname: Yang
authors.forename: Shiuan-Chih
authors.initials: SC
authors.lastname: Chen
authors.forename: Horng-Jyh
authors.initials: HJ
authors.lastname: Tsai
authors.forename: Long-Yau
authors.initials: LY
authors.lastname: Lin
authors.forename: Po-Hui
authors.initials: PH
authors.lastname: Wang
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000201:enzymology*; D005260:Female; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000097:blood*; D046888:Tissue Array Analysis; D002583:Uterine Cervical Neoplasms / Q000201:enzymology*; D002578:Uterine Cervical Dysplasia / Q000201:enzymology*
chemical_list: D020778:Matrix Metalloproteinase 2