Differential ionisation of natural antioxidant polyenes in electrospray and nanospray mass spectrometry.
Carotenoids are natural products with high economic relevance for the pharmaceutical industries and are a common subject for biochemical research. Reported here is a comparative study of the ionisation of carotenoids by electrospray mass spectrometry (ESI-MS) and nanospray mass spectrometry (nanoESI-MS). The results demonstrate that, along with solvent choice, the influence of the different ionisation processes of ESI and nanoESI are fundamental in determining how ionisation is achieved and which ions (molecular ion or protonated molecule) are observed in MS. The increased understanding afforded by this study will help in the development of unequivocal microanalytical methods for carotenoids and related antioxidant polyenes.
Guaratini
Thais
T
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av Professor Lineu Prestes, 748 CP 20780, CEP 05508900, São Paulo-SP, Brazil.
Gates
Paul J
PJ
Pinto
Ernani
E
Colepicolo
Pio
P
Lopes
Norberto P
NP
D000975:Antioxidants / Q000032:analysis*; D001685:Biological Factors / Q000032:analysis*; D044085:Microfluidics / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D011090:Polyenes / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000975:Antioxidants; D001685:Biological Factors; D011090:Polyenes