Optimization of beta-carotene production by Rhodotorula glutinis using high hydrostatic pressure and response surface methodology.
Rhodotorula glutinis RG6 was treated by high hydrostatic pressure (HHP) of 300 MPa for 15 min for improving its ability of beta-carotene production. After the treatments of 5 repeated cycles, the mutant strain RG6p was obtained, beta-carotene production of which reached 10.01 mg/L, increased by 57.89% compared with 6.34 mg/L from parent strain RG6. To optimize the medium for beta-carotene fermentation by mutant RG6p, a response surface methodology (RSM) approach was used in conjunction with a factorial design and a central composite design, and the maximum yield of beta-carotene (13.43 mg/L), an increase of 34.17% compared to the control, was obtained at a pH 6.7 with an optimum medium (40 mL/250 mL) of yeast extract (4.23 g/L), glucose (12.11 g/L), inoculum (30 mL/L), tomato extract (2.5 mL/L), peanut oil (0.5 mL/L), and (NH(4))(2)SO(4) (5 g/L).
Wang
S-L
SL
College of Food Science and Nutritional Engineering, China Agricultural Univ., Beijing 100083, P.R. China.
Sun
J-S
JS
Han
B-Z
BZ
Wu
X-Z
XZ
D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006874:Hydrostatic Pressure; D007218:Industrial Microbiology / Q000379:methods*; D009154:Mutation; D000074241:Peanut Oil; D010938:Plant Oils / Q000378:metabolism; D012248:Rhodotorula / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D019207:beta Carotene / Q000096:biosynthesis*
D003470:Culture Media; D000074241:Peanut Oil; D010938:Plant Oils; D019207:beta Carotene; D005947:Glucose