title: Surface activity, lipid profiles and their implications in cervical cancer.
abstract: BACKGROUND
  The profiles of lipids in normal and cancerous tissues may differ revealing information about cancer development and progression. Lipids being surface active, changes in lipid profiles can manifest as altered surface activity profiles. Langmuir monolayers offer a convenient model for evaluating surface activity of biological membranes.
  
  
  OBJECTIVE
  The aims of this study were to quantify phospholipids and their effects on surface activity of normal and cancerous human cervical tissues as well as to evaluate the role of phosphatidylcholine (PC) and sphingomyelin (SM) in cervical cancer using Langmuir monolayers.
  
  
  METHODS
  Lipid quantification was done using thin layer chromatography and phosphorus assay. Surface activity was evaluated using Langmuir monolayers. Monolayers were formed on the surface of deionized water by spreading tissue organic phase corresponding to 1 mg of tissue and studying their surface pressure-area isotherms at body temperature. The PC and SM contents of cancerous human cervical tissues were higher than those of the normal human cervical tissues. Role of PC and SM were evaluated by adding varying amounts of these lipids to normal cervical pooled organic phase.
  
  
  METHODS
  Student's t-test (p < 0.05) and one-way analysis of variance (ANOVA) was used.
  
  
  RESULTS
  Our results reveals that the phosphatidylglycerol level in cancerous cervical tissue was nearly five folds higher than that in normal cervical tissue. Also PC and sphingomyelin SM were found to be the major phospholipid components in cancerous and normal cervical tissues respectively. The addition of either 1.5 microg DPPC or 0.5 microg SM /mg of tissue to the normal organic phase changed its surface activity profile to that of the cancerous tissues. Statistically significant surface activity parameters showed that PC and SM have remarkable roles in shifting the normal cervical lipophilic surface activity towards that of cancerous lipophilic component.
  
  
  CONCLUSIONS
  The Langmuir monolayer technique was sensitive to detect changes in tensiometric profiles of cervical cancers and these could be modulated by alterations in phosphatidylcholine and sphingomyelin levels. Therapeutic strategies may be designed to modulate these tensiometric profiles and lipid constituents of cancerous tissues.
authors.affiliation: School of Biosciences and Bioengineering, Indian Institute of Technology, Mumbai-400076, India.
authors.forename: A
authors.initials: A
authors.lastname: Preetha
authors.forename: R
authors.initials: R
authors.lastname: Banerjee
authors.forename: Nagraj
authors.initials: N
authors.lastname: Huilgol
mesh_terms: D000731:Androstanes / Q000032:analysis; D002584:Cervix Uteri / Q000737:chemistry; D002855:Chromatography, Thin Layer; D005260:Female; D006801:Humans; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D013109:Sphingomyelins / Q000032:analysis; D013499:Surface Properties; D002583:Uterine Cervical Neoplasms / Q000737:chemistry*
chemical_list: C415601:3-oxo-androstan-17-yl-(2'',2'',6'',6''-tetramethyl-N-oxyl)piperidyl butan-1',4'-dioate; D000731:Androstanes; D010713:Phosphatidylcholines; D010743:Phospholipids; D013109:Sphingomyelins