Separation and determination of flavonoids in Ixeridium gracile by capillary electrophoresis.
A simple and rapid capillary electrophoresis method has been developed for the quantitative analysis of three active compounds: (3R)-7,2'-dihydroxy-3',4'-dimethoxy-isoflavan, kaempferol, and quercetin in Ixeridium gracile. The buffer solution used in this method is 20 mmol/L borate at pH 9.5. The effects of pH value, borate concentration, and applied voltage on migration behavior are systematically investigated. Regression equations reveal good linear relationships (correlation coefficients: 0.9986, 0.9997, and 0.9999) between the peak area of each compound and its concentration. The relative standard deviations of the migration time and peak area are less 1.12% and 3.11% (intraday), and 1.43% and 3.52% (interday), respectively, under the optimized separation conditions. The contents of the three flavonoids in I. gracile are successfully determined within 7.8 min, with satisfactory repeatability and recovery.
Zhang
Yu
Y
Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, P.R. China.
Zhao
Liang
L
Shi
Yan-Ping
YP
D019659:Asteraceae / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification*; D038861:Medicine, Tibetan Traditional; D015203:Reproducibility of Results
D005419:Flavonoids