Consequences of changes in BDNF levels on serotonin neurotransmission, 5-HT transporter expression and function: studies in adult mice hippocampus.
In vivo intracerebral microdialysis is an important neurochemical technique that has been applied extensively in genetic and pharmacological studies aimed at investigating the relationship between neurotransmitters. Among the main interests of microdialysis application is the infusion of drugs through the microdialysis probe (reverse dialysis) in awake, freely moving animals. As an example of the relevance of intracerebral microdialysis, this review will focus on our recent neurochemical results showing the impact of Brain-Derived Neurotrophic Factor (BDNF) on serotonergic neurotransmission in basal and stimulated conditions. Indeed, although the elevation of 5-HT outflow induced by chronic administration of selective serotonin reuptake inhibitors (SSRIs) causes an increase in BDNF protein levels and expression (mRNA) in the hippocampus of rodents, the reciprocal interaction has not been demonstrated yet. Thus, the neurochemical sight of this question will be addressed here by examining the consequences of either a constitutive decrease or increase in brain BDNF protein levels on hippocampal extracellular levels of 5-HT in conscious mice.
Deltheil
Thierry
T
Univ. Paris Sud, EA 3544, Fac. Pharmacie, Chatenay-Malabry cedex F-92296, France.
Guiard
Bruno P
BP
Guilloux
Jean-Philippe
JP
Nicolas
Lorelei
L
Deloménie
Claudine
C
Repérant
Christelle
C
Le Maitre
Erwan
E
Leroux-Nicollet
Isabelle
I
Benmansour
Saloua
S
Coudoré
François
F
David
Denis J
DJ
Gardier
Alain M
AM
D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology / Q000502:physiology*; D015283:Citalopram / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D051379:Mice; D017551:Microdialysis / Q000379:methods*; D017374:Paroxetine / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D012701:Serotonin / Q000378:metabolism*; D050486:Serotonin Plasma Membrane Transport Proteins / Q000235:genetics* / Q000502:physiology
D000928:Antidepressive Agents; D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D050486:Serotonin Plasma Membrane Transport Proteins; D015283:Citalopram; D012701:Serotonin; D017374:Paroxetine