title: Implementation of electrochemiluminescence microanalysis in PCB technology.
abstract: We present an instrumental development to implement electrochemiluminescence (ECL) microanalysis using printed circuit board (PCB) technology. PCB gold macro-(10 mm2) and micro- (0.09 mm2) electrodes and two ECL microfluidic devices are designed, fabricated and tested via luminol ECL detection. Potential modulation is performed between 0.7 and 0 V vs. Ag/AgCl for luminol oxidation, thus giving rise to on/off ECL responses in the presence of hydrogen peroxide. Synchronous detection is adopted to allow weak ECL signal recovery at a very low signal-to-noise ratio (SNR). The detection limit obtained with the two ECL microfluidic devices is 50 nM and 100 nM H2O2 for macroelectrodes and microelectrodes, respectively.
authors.affiliation: INL Institut des Nanotechnologies de Lyon, 43 boulevard du 11 novembre 1918, Villeurbanne, F-69622, France. Patrick.Pittet@lenac.univ-lyon1.fr
authors.forename: Patrick
authors.initials: P
authors.lastname: Pittet
authors.forename: Guo-Neng
authors.initials: GN
authors.lastname: Lu
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Galvan
authors.forename: Rosaria
authors.initials: R
authors.lastname: Ferrigno
authors.forename: Khaled
authors.initials: K
authors.lastname: Stephan
authors.forename: Loïc J
authors.initials: LJ
authors.lastname: Blum
authors.forename: Béatrice D
authors.initials: BD
authors.lastname: Leca-Bouvier
mesh_terms: D004563:Electrochemistry; D004581:Electronics; D006861:Hydrogen Peroxide / Q000737:chemistry; D049408:Luminescent Agents / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques; D012834:Silver / Q000737:chemistry; D018030:Silver Compounds / Q000737:chemistry
chemical_list: D049408:Luminescent Agents; D018030:Silver Compounds; D012834:Silver; D008165:Luminol; D006861:Hydrogen Peroxide; C037548:silver chloride