title: Polyethylene glycol (PEG)-carboxylate-CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103.
abstract: Sphingopyxis macrogoltabida strain 103 possesses polyethylene-glycol (PEG)-inducible pegBCDAE operon encoding the genes relevant to PEG degradation. PEG is converted to PEG-carboxylate by PegA (PEG dehydrogenase) and PegC (PEG-aldehyde dehydrogenase). In this study, the recombinant PegE (homologous to acyl-CoA synthetases) was characterized. PegE was an acyl-CoA synthetase active for PEG-carboxylate and fatty acids. Judging from the nature of this kind of protein (located on the cytoplasmic membrane as a translocator), PegE might be responsible for the translocation of PEG-carboxylate from the periplasm into the cytoplasm or for the detoxification of strong acidity of the substrate.
authors.affiliation: Research Institute for Bioresources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama 710-0046, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Tani
authors.forename: Peechapack
authors.initials: P
authors.lastname: Somyoonsap
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Minami
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Kimbara
authors.forename: Fusako
authors.initials: F
authors.lastname: Kawai
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003066:Coenzyme A Ligases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D009876:Operon; D011092:Polyethylene Glycols / Q000378:metabolism*; D042301:Sphingomonadaceae / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D005227:Fatty Acids; D011092:Polyethylene Glycols; D003066:Coenzyme A Ligases