Evaluation of the broth microdilution method using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride for rapidly growing mycobacteria susceptibility testing.
As the incidence of nontuberculous mycobacterial infection has been increasing recently in Korea, the importance of drug susceptibility test for clinical isolates of mycobacteria has become larger. In this study we determined the antimicrobial susceptibility patterns of clinical isolates of M. fortuitum and M. abscessus in Korea, and evaluated the efficacy of a modified broth microdilution method using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride (STC), in terms of its ability to provide accurate and easy-to-read minimal inhibitory concentration (MIC) endpoints for the susceptibility testing of rapidly growing mycobacteria. Most isolates of M. fortuitum and M. abscessus in Korea are susceptible or intermediately susceptible to amikacin, cefoxitin, ciprofloxacin, and clarithromycin. Many isolates of M. fortuitum are susceptible to doxycycline, sulfamethoxazole, and imipenem, while many M. abscessus isolates are resistant to these drugs. In the present study, the modified broth microdilution method using STC was found to be reliable, easy to read, and inexpensive for M. fortuitum and M. abscessus susceptibility testing. The modified colorimetric MIC testing method using STC was proven to be a useful surrogate for RGM antibiotic susceptibility testing.
Lee
Sun Min
SM
Department of Laboratory Medicine, School of Medicine, Pusan National University, Busan, Korea.
Kim
Jeong man
Jm
Jeong
Joseph
J
Park
Young Kil
YK
Bai
Gill-Han
GH
Lee
Eun Yup
EY
Lee
Min Ki
MK
Chang
Chulhun L
CL
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002440:Cefoxitin / Q000494:pharmacology; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002939:Ciprofloxacin / Q000494:pharmacology; D017291:Clarithromycin / Q000494:pharmacology; D003124:Colorimetry / Q000379:methods; D024881:Drug Resistance, Bacterial; D007723:Korea; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000378:metabolism*; D019907:Mycobacterium fortuitum / Q000378:metabolism*; D013778:Tetrazolium Salts / Q000494:pharmacology*
C494145:2,3-diphenyl-5-thienyl-(2)-tetrazolium; D000900:Anti-Bacterial Agents; D013778:Tetrazolium Salts; D002939:Ciprofloxacin; D002440:Cefoxitin; D017291:Clarithromycin