Sequence-selective extraction of single-stranded DNA using DNA-functionalized reverse micelles.
We report here sequence-specific liquid/liquid extraction of single-stranded DNA using reverse micelles (water-in-oil microemulsions), in which hybridization between a DNA-surfactant and a target DNA having a complementary sequence allows selective transport of the target DNA to an organic phase from a mixture of DNA oligonucleotides.
Maruyama
Tatsuo
T
Department of Applied Chemistry, Graduate School of Engineering and Center for Future Chemistry, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan. tmarutcm@mbox.nc.kyushu-u.ac.jp
Hosogi
Takuya
T
Goto
Masahiro
M
D004277:DNA, Single-Stranded / Q000737:chemistry / Q000302:isolation & purification*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008823:Micelles; D013501:Surface-Active Agents / Q000737:chemistry*
D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D008823:Micelles; D013501:Surface-Active Agents; D016650:Fluorescein-5-isothiocyanate