title: Creation of intercellular bonds by anchoring protein ligands to membranes using the diphtheria toxin T domain.
abstract: We describe the creation of cell adhesion mediated by cell surface engineering. The Flt3-ligand was fused to a membrane anchor made of the diphtheria toxin translocation domain. The fusion protein was attached to the surface of a cell by an acid pulse. Contact with another cell expressing the receptor Flt3 lead to its activation. This activity involved direct cell-cell contact. A mean force of 20 nN was needed to separate functionalized cells after 5 min of contact. Overall, we showed that it is possible to promote specific cell-cell adhesion by attaching protein ligands at the surface of cells.
authors.affiliation: Commissariat à l'Energie Atomique, DSV, iBiTecS, SIMOPRO, CEA-Saclay, F-91191 Gif sur Yvette, France. aurelieperier@hotmail.com
authors.forename: Aurélie
authors.initials: A
authors.lastname: Perier
authors.forename: Christine
authors.initials: C
authors.lastname: Gourier
authors.forename: Sylvain
authors.initials: S
authors.lastname: Pichard
authors.forename: Julien
authors.initials: J
authors.lastname: Husson
authors.forename: Evelyne
authors.initials: E
authors.lastname: Lajeunesse
authors.forename: Aurélie
authors.initials: A
authors.lastname: Babon
authors.forename: André
authors.initials: A
authors.lastname: Menez
authors.forename: Daniel
authors.initials: D
authors.lastname: Gillet
mesh_terms: D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D004167:Diphtheria Toxin / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding
chemical_list: D004167:Diphtheria Toxin; D008024:Ligands; D008565:Membrane Proteins; C084881:flt3 ligand protein