Two novel biomarkers in developing wood frogs.
Two novel biomarkers (whole-body thyroid hormone and triglyceride concentrations) were developed for use in wood frog tadpoles. The development of these biomarkers was based on previous work done in fish and lizards and incorporated triglyceride and thyroid hormone extraction into 1 homogenization step with 95% ethanol as the solvent. This method is useful for reducing sample-to-sample variability because the same sample can be used for each extraction and assay.
Gupta
Niti
N
University of Saskatchewan, Saskatoon, Saskatchewan, Canada. NJG792@mail.usask.ca
Hitchings
Amanda
A
Weber
Lynn
L
Wickstrom
Mark
M
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004784:Environmental Monitoring; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D011898:Ranidae / Q000196:embryology / Q000378:metabolism*; D013963:Thyroid Hormones / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism*; D014873:Water Pollutants / Q000633:toxicity*
D015415:Biomarkers; D013963:Thyroid Hormones; D014280:Triglycerides; D014873:Water Pollutants