title: Gene expression profile related to prognosis of acute myeloid leukemia.
abstract: Acute myeloid leukemia (AML) is a heterogeneous group of diseases with respect to biology and clinical course. Through genome-wide scanning, we can have an improvement of the diagnosis and assay system of AML. Microarray was performed for the identification of acute myeloid leukemia prognosis. We divided patients into two groups (good prognosis group, GPG and poor prognosis group, PPG) based on differences in the individual reactions to treatment. Gene expression profiles were analyzed using microarray. Among genes up-regulated at least two-fold and down-regulated at least 0.5-fold in HL-60, we chose three up-regulated genes (PPP2CA, ME3, and CCDN2) and three down-regulated genes (GLO1, ANXA2, and BMI1) and confirmed the expression of these six genes by RT-PCR. We created a leukemia-specific subclass microarray, based on the gene expression profiles. Clinical samples from the bone marrow of four patients were hybridized on this microarray. Among the genes selected by the microarray technology, NB4, silenced TRIB3 and overexpressed XRN2 were not differentiated in spite of treatment with ATRA. This indicates that XRN2 and TRIB3 play an important role in cell differentiation. These data provided an expression profile for the diagnosis and prognosis of AML patients and identified candidate genes that might allow the prognosis of AML through the relative comparison of the expression level of genes between GPG and PPG.
authors.affiliation: Biological Science, Sookmyung Women's University, Seoul 140-742, Korea.
authors.forename: Min Ha
authors.initials: MH
authors.lastname: Park
authors.forename: Sun A
authors.initials: SA
authors.lastname: Cho
authors.forename: Kyung Hyun
authors.initials: KH
authors.lastname: Yoo
authors.forename: Moon Hee
authors.initials: MH
authors.lastname: Yang
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Ahn
authors.forename: Hyo Soo
authors.initials: HS
authors.lastname: Lee
authors.forename: Kyoung-Eun
authors.initials: KE
authors.lastname: Lee
authors.forename: Yeung-Chul
authors.initials: YC
authors.lastname: Mun
authors.forename: Dae Ho
authors.initials: DH
authors.lastname: Cho
authors.forename: Chu Myong
authors.initials: CM
authors.lastname: Seong
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Park
mesh_terms: D017306:Annexin A2 / Q000235:genetics; D045744:Cell Line, Tumor; D015536:Down-Regulation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D018922:HL-60 Cells; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D054648:Protein Phosphatase 2 / Q000235:genetics; D015854:Up-Regulation
chemical_list: C472822:ANXA2 protein, human; D017306:Annexin A2; C517189:PPP2CA protein, human; D054648:Protein Phosphatase 2