title: [Ocular onchocerciasis: a key role for Wolbachia].
abstract: Onchocerciasis is caused by the parasitic worm Onchocerca volvulus, which releases millions of offspring (microfilariae). Microfilariae migrate through the skin and can enter the anterior or posterior regions of the eye. While alive, the microfilariae appear to cause little or no inflammation, being in the anterior chamber. However, when they die, either by natural attrition or after chemotherapy, the host response to degenerating worms can result in ocular inflammation (keratitis, uveitis, chorioretinitis, neuritis of the optic nerve) that causes progressive loss of vision and ultimately leads to blindness. With the use of a mouse model of corneal inflammation to study the pathogenesis of ocular onchocerciasis by injecting worm extracts directly into the corneal stroma, it was found that worms treated with the antibiotic doxycycline, which destroys Wolbachia, induced lower corneal stromal thickness and stromal haze (indicators of corneal oedema and opacity) and neutrophil infiltration compared with both untreated worms and worms that do not harbour Wolbachia. These data indicate that endosymbiotic Wolbachia bacteria in filarial parasites have a key role in the pathogenesis of river blindness. Worms recovered from patients treated for 6 weeks with doxycycline contained fewer Wolbachia bacteria and had abnormal embryogenesis, indicating a role for Wolbachia in the survival or fecundity of the worms. Antibiotic treatment may also reduce the severity of the inflammatory response in the cornea.
authors.affiliation: Augenärztliche Gemeinschaftspraxis, Brückenweg 1, 42929, Wermelskirchen. gkluxen@t-online.de
authors.forename: G
authors.initials: G
authors.lastname: Kluxen
authors.forename: A
authors.initials: A
authors.lastname: Hörauf
mesh_terms: D000818:Animals; D015715:Corneal Edema / Q000276:immunology / Q000382:microbiology; D003318:Corneal Opacity / Q000276:immunology / Q000382:microbiology; D004195:Disease Models, Animal; D004318:Doxycycline / Q000494:pharmacology; D006790:Host-Parasite Interactions; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology; D051379:Mice; D008842:Microfilariae / Q000276:immunology / Q000382:microbiology*; D009504:Neutrophils / Q000276:immunology; D017181:Onchocerca volvulus / Q000276:immunology / Q000382:microbiology*; D015827:Onchocerciasis, Ocular / Q000276:immunology / Q000382:microbiology*; D012288:Rickettsiaceae Infections / Q000276:immunology / Q000382:microbiology*; D013559:Symbiosis; D014774:Virulence / Q000187:drug effects / Q000276:immunology; D020577:Wolbachia / Q000187:drug effects / Q000276:immunology / Q000472:pathogenicity*
chemical_list: D004318:Doxycycline