A mycobacterial smc null mutant is proficient in DNA repair and long-term survival.
SMC (structural maintenance of chromosomes) proteins play fundamental roles in various aspects of chromosome organization and dynamics, including repair of DNA damage. Mutant strains of Mycobacterium smegmatis and Mycobacterium tuberculosis defective in SMC were constructed. Surprisingly, inactivation of smc did not result in recognizable phenotypes in hallmark assays characteristic for the function of these genes. This is in contrast to data for smc null mutants in other species.
Güthlein
Carolin
C
Institut für Medizinische Mikrobiologie, Universität Zürich, Gloriastrasse 30/32, 8006 Zurich, Switzerland. cguethl@immv.uzh.ch
Wanner
Roger M
RM
Sander
Peter
P
Böttger
Erik C
EC
Springer
Burkhard
B
D001426:Bacterial Proteins / Q000235:genetics*; D015139:Blotting, Southern; D018797:Cell Cycle Proteins / Q000235:genetics*; D002470:Cell Survival; D002876:Chromosomes, Bacterial / Q000235:genetics / Q000648:ultrastructure; D017124:Conserved Sequence; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics*; D017353:Gene Deletion; D009154:Mutation; D009161:Mycobacterium / Q000235:genetics*; D020102:Mycobacterium smegmatis / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics*
D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; C118650:SMC protein, Bacteria