Position dependence of hemiray morphogenesis during tail fin regeneration in Danio rerio.
The fins of actinopterygian can regenerate following amputation. Classical papers have shown that the ray, a structural unit of these fins, might regenerate independent of this appendage. Each fin ray is formed by two apposed contralateral hemirays. A hemiray may autonomously regenerate and segmentate in a position-independent manner. This is observed when heterotopically grafted into an interray space, after amputation following extirpation of the contralateral hemiray or when simply ablated. During this process, a proliferating hemiblastema is formed, as shown by bromodeoxyuridine incorporation, from which the complete structure will regenerate. This hemiblastema shows a patterning of gene expression domain similar to half ray blastema. Interactions between contralateral hemiblastema have been studied by recombinant rays composed of hemirays from different origins on the proximo-distal or dorso-ventral axis of the caudal fin. Dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocianine perchlorate labeling of grafted tissues was used as tissular marker. Our results suggest both that there are contralateral interactions between hemiblastema of each ray, and that hemiblastema may vary its morphogenesis, always differentiating as their host region. These non-autonomous, position-dependent interactions control coordinated bifurcations, segment joints and ray length independently. A morphological study of the developing and regenerating fin of another long fin mutant zebrafish suggests that contralateral hemiblastema interactions are perturbed in this mutant.
Murciano
C
C
Department of Cell Biology, Genetics and Physiology, Faculty of Science, University of Málaga, 29071-Málaga, Spain.
Pérez-Claros
J
J
Smith
A
A
Avaron
F
F
Fernández
T D
TD
Durán
I
I
Ruiz-Sánchez
J
J
García
F
F
Becerra
J
J
Akimenko
M-A
MA
Marí-Beffa
M
M
D000825:Animal Structures / Q000166:cytology / Q000254:growth & development*; D000818:Animals; D019521:Body Patterning; D001973:Bromodeoxyuridine / Q000378:metabolism; D049109:Cell Proliferation; D018507:Gene Expression Regulation, Developmental; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D012038:Regeneration; D013623:Tail / Q000033:anatomy & histology / Q000254:growth & development*; D015027:Zebrafish / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development*; D029961:Zebrafish Proteins / Q000235:genetics
D029961:Zebrafish Proteins; D001973:Bromodeoxyuridine