title: Influence of the sporulation temperature on the impact of the nutrients inosine and l-alanine on Bacillus cereus spore germination.
abstract: The effect of sporulation temperature on Bacillus cereus spore germination triggered by the nutrient germinants inosine and l-alanine was investigated. The germination (expressed as % A(600nm) fall) of heat-activated spores of B. cereus strain ATCC14579 produced at 20 and 37 degrees C, and of psychrotrophic strains LM9 and D15 produced at 15 and 37 degrees C was followed in a germination buffer containing inosine at concentrations between 0.01 and 10mmoll(-1), or l-alanine between 1 and 100mmoll(-1). Spores wet-heat resistance at 90 degrees C was also determined. Spores of the three strains produced at the lowest temperatures generally showed a higher germination capacity in response to both inosine and in l-alanine than those produced at 37 degrees C, and were also more susceptible to heat. Low sporulation temperature is confirmed as a detrimental factor to B. cereus spore wet-heat resistance, and conversely gave spores with a sensitivity to the nutrient germinants inosine and l-alanine higher than that of spores formed close to the maximal sporulation temperature.
authors.affiliation: UMR 408 Sécurité et Qualité des Produits d'Origine Végétale, INRA, Université d'Avignon, Site Agroparc, F-84000 Avignon, Cedex, France.
authors.forename: Rabia
authors.initials: R
authors.lastname: Gounina-Allouane
authors.forename: Véronique
authors.initials: V
authors.lastname: Broussolle
authors.forename: Frédéric
authors.initials: F
authors.lastname: Carlin
mesh_terms: D000409:Alanine / Q000494:pharmacology*; D001409:Bacillus cereus / Q000502:physiology*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004305:Dose-Response Relationship, Drug; D005516:Food Microbiology; D007288:Inosine / Q000494:pharmacology*; D013171:Spores, Bacterial / Q000187:drug effects* / Q000254:growth & development; D013696:Temperature; D013997:Time Factors
chemical_list: D007288:Inosine; D000409:Alanine