Analysis of toxigenic Corynebacterium ulcerans strains revealing potential for false-negative real-time PCR results.
Diphtheria surveillance depends on the rapid and reliable recognition of the toxin gene in Corynebacterium diphtheriae. Real-time PCR is a rapid tool to confirm the presence of the diphtheria toxin gene (tox) in an isolate or specimen. We report that some toxigenic Corynebacterium ulcerans strains show atypical results in a real-time PCR for tox.
Cassiday
Pamela K
PK
National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop D-11, Atlanta, GA 30333, USA. PCassiday@cdc.gov
Pawloski
Lucia C
LC
Tiwari
Tejpratap
T
Sanden
Gary N
GN
Wilkins
Patricia P
PP
D003352:Corynebacterium / Q000235:genetics* / Q000302:isolation & purification; D003354:Corynebacterium Infections / Q000175:diagnosis / Q000382:microbiology*; D004167:Diphtheria Toxin / Q000032:analysis* / Q000235:genetics*; D005188:False Negative Reactions; D006801:Humans; D047468:Immunoprecipitation; D010614:Pharynx / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D017422:Sequence Analysis, DNA
D004167:Diphtheria Toxin