Superparamagnetic gamma-Fe(2)O(3) nanoparticles with tailored functionality for protein separation.
Polymer coated superparamagnetic gamma-Fe(2)O(3) nanoparticles were derivatized with a synthetic double-stranded RNA [poly(IC)], a known allosteric activator of the latent (2-5)A synthetase, to separate a single 35 kDa protein from a crude extract which cross reacted with antibodies raised against the sponge enzyme.
Shukoor
Mohammed Ibrahim
MI
Institut für Anorganische Chemie und Analytische Chemie, Universität Mainz, Duesbergweg 10-14, D-55099, Mainz, Germany.
Natalio
Filipe
F
Tahir
Muhammad Nawaz
MN
Ksenofontov
Vadim
V
Therese
Helen Annal
HA
Theato
Patrick
P
Schröder
Heinz C
HC
Müller
Werner E G
WE
Tremel
Wolfgang
W
D015088:2',5'-Oligoadenylate Synthetase / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D001665:Binding Sites; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D018189:Immunomagnetic Separation; D008024:Ligands; D008280:Magnetics; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*; D011161:Porifera / Q000201:enzymology; D013499:Surface Properties
D005290:Ferric Compounds; D008024:Ligands; D010455:Peptides; C000499:ferric oxide; D012321:DNA-Directed RNA Polymerases; D015088:2',5'-Oligoadenylate Synthetase