title: Effect of lifelong coenzyme Q10 supplementation on age-related oxidative stress and mitochondrial function in liver and skeletal muscle of rats fed on a polyunsaturated fatty acid (PUFA)-rich diet.
abstract: This study investigates aging-related changes in lipid peroxidation and functionality in liver and skeletal-muscle mitochondria in rats fed a diet rich in polyunsaturated fatty acids (PUFA), depending on supplementation or not with coenzyme Q(10) (CoQ(10)). Two groups of rats were fed for 24 months on a PUFA-rich diet, differing in supplementation or not with CoQ(10). At 6 and 24 months mitochondria were analyzed for fatty acid profile; hydroperoxides; alpha-tocopherol; CoQ(9;) CoQ(10;) cytochromes b, c+c(1), and a+a(3) contents; cytochrome c oxidase activity; and catalase activity in cytosol. Results of this study showed for the supplemented group an age-associated decrease in the peroxidizability index, an increase in catalase activity in skeletal muscle, and modulation of the aging-related changes in different mitochondrial electron-transport-chain components in skeletal muscle. These findings provide mechanisms to explain the effect of CoQ(10) in extending the life span of animals fed a PUFA-rich diet.
authors.affiliation: Department of Physiology, University of Granada, Granada, Spain. jjoh@ugr.es
authors.forename: Julio J
authors.initials: JJ
authors.lastname: Ochoa
authors.forename: José L
authors.initials: JL
authors.lastname: Quiles
authors.forename: Magdalena
authors.initials: M
authors.lastname: López-Frías
authors.forename: Jesús R
authors.initials: JR
authors.lastname: Huertas
authors.forename: José
authors.initials: J
authors.lastname: Mataix
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D002374:Catalase / Q000378:metabolism; D003067:Coenzymes / Q000008:administration & dosage / Q000494:pharmacology; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage; D003576:Electron Transport Complex IV / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage*; D015227:Lipid Peroxidation; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D024502:alpha-Tocopherol / Q000378:metabolism
chemical_list: D003067:Coenzymes; D004042:Dietary Fats, Unsaturated; D005231:Fatty Acids, Unsaturated; D014451:Ubiquinone; D002374:Catalase; D003576:Electron Transport Complex IV; C024989:coenzyme Q10; D024502:alpha-Tocopherol