The role of hypothalamic peptide gene expression in alcohol self-administration behavior.
Self-administration of ethanol and food share many common features and Richter hypothesized that an increase in ethanol consumption would decrease feeding to balance the excess calories contained in the ethanol. Previously, we have shown that individual alcohol consumption correlates with neurotransmitter gene expression, especially in the prefrontal cortex. To test the hypothesis of Richter, we measured hypothalamic gene expression of receptors or neuropeptides of known relevance for the regulation of food intake using qPCR and correlated this to individual ethanol consumption in Wistar rats. For validation, gene expression was first correlated with body weight. We found a correlation of dynorphin, somatostatin, melanocortin-4 receptor and serotonin 5-HT(2C) with body weight and trends to correlation for CART, thus confirming the established role of the hypothalamus in the regulation of weight. For ethanol consumption, correlations were found for CRH receptors 1 and 2 and vasopressin while strong trends were observed for galanin receptor 1, orexin receptor 1, MCH and adrenoceptor alpha(1B). Therefore, alcohol consumption does seem to involve several hypothalamic systems which also mediate feeding responses and suggests that the hypothalamus, together with the prefrontal cortex, may determine the 'stopping point' of an individual.
Pickering
Chris
C
Uppsala University, Department of Neuroscience, Division of Pharmacology, BMC, Box 593, SE-75124 Uppsala, Sweden. Chris.Pickering@neuro.uu.se
Avesson
Lotta
L
Liljequist
Sture
S
Lindblom
Jonas
J
Schiöth
Helgi B
HB
D000428:Alcohol Drinking / Q000235:genetics* / Q000378:metabolism / Q000523:psychology; D000818:Animals; D001127:Arginine Vasopressin / Q000235:genetics; D001483:Base Sequence; D001835:Body Weight / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004435:Eating / Q000235:genetics; D015870:Gene Expression; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D009479:Neuropeptides / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018019:Receptors, Corticotropin-Releasing Hormone / Q000235:genetics; D012646:Self Administration
D017931:DNA Primers; D009479:Neuropeptides; D012333:RNA, Messenger; D018019:Receptors, Corticotropin-Releasing Hormone; D001127:Arginine Vasopressin