Isolation and characterization of l-rhamnose-binding lectin, which binds to microsporidian Glugea plecoglossi, from ayu (Plecoglossus altivelis) eggs.
A rhamnose-binding lectin, named SFL, was isolated from the eggs of ayu (sweet fish, Plecoglossus altivelis) by affinity and ion-exchange chromatographies. SFL revealed 287 amino acid residues with 3 tandemly repeated domains, and contained 8 half-Cys residues in each domain. The lectin was shown to have a highly specific binding affinity to globotriaosylceramide (Gb3) by frontal affinity chromatography using 100 oligosaccharides. SFL was localized in several tissues and serum of both male and female ayu, such as gill, liver, ovary, testis, intestine, stomach, brain, kidney and serum. The lectin agglutinated the spores of the microsporidian Glugea plecoglossi, which is a pathogen of ayu. Although SFL bound to glycoproteins and glycolipids of G. plecoglossi spores, Gb3 could not be detected in either of them. The results suggest that SFL could interact with various glycoconjugates of pathogens to play a role in the adhesion of microorganisms invading in the body.
Watanabe
Yasuharu
Y
Department of Biomolecular Science, Graduate School of Life Sciences, Tohoku University, Sendai 981-8555, Japan.
Shiina
Nobuyuki
N
Shinozaki
Fuminori
F
Yokoyama
Hiroshi
H
Kominami
Junko
J
Nakamura-Tsuruta
Sachiko
S
Hirabayashi
Jun
J
Sugahara
Kazuhiro
K
Kamiya
Hisao
H
Matsubara
Hiroki
H
Ogawa
Tomohisa
T
Muramoto
Koji
K
D000595:Amino Acid Sequence; D000818:Animals; D029941:Fish Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D007113:Immunity, Innate; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D016814:Microsporidia / Q000378:metabolism*; D008969:Molecular Sequence Data; D036441:Osmeriformes / Q000276:immunology* / Q000469:parasitology; D010063:Ovum; D010802:Phylogeny; D012210:Rhamnose / Q000378:metabolism*; D033761:Spores, Protozoan / Q000378:metabolism
D029941:Fish Proteins; D037102:Lectins; D012210:Rhamnose