Prolactin stimulates the internalization of Staphylococcus aureus and modulates the expression of inflammatory response genes in bovine mammary epithelial cells.
The incidence of mastitis in dairy cattle is highest at the drying off period and parturition, which are characterized by high levels of the lactogenic hormone prolactin (PRL). One of the most frequently isolated contagious pathogens causing mastitis is Staphylococcus aureus. However, the role of PRL on S. aureus infection in mammary epithelium has not been studied. In this work we evaluated the effect of bovine PRL (bPRL) on S. aureus internalization in a primary culture of bovine mammary epithelial cells (bMEC) and on the expression of cytokine and innate immune response genes. Our data show that 5ng/mL bPRL enhances approximately 3-fold the internalization of S. aureus (ATCC 27543) into bMEC. By RT-PCR analysis, we showed that bPRL is able to up-regulate the expression of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta) and inducible nitric oxide synthase (iNOS) mRNAs. However, bPRL together with S. aureus did not modify the expression of TNF-alpha and iNOS mRNAs, while it down-regulated the expression of beta-defensin and IL-1beta mRNAs, as well as nitric oxide production, suggesting that infection and bPRL together can inhibit elements of the host immune response. To our knowledge, this is the first report that shows a role of bPRL during the internalization of S. aureus into bMEC.
Gutiérrez-Barroso
Angelina
A
Centro Multidisciplinario de Estudios en Biotecnología-Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Apdo. Postal 53, Administración Chapultepec, C.P. 58262, Morelia, Michoacán, México.
Anaya-López
José L
JL
Lara-Zárate
Leticia
L
Loeza-Lara
Pedro D
PD
López-Meza
Joel E
JE
Ochoa-Zarzosa
Alejandra
A
D000818:Animals; D002417:Cattle; D004847:Epithelial Cells / Q000187:drug effects / Q000382:microbiology; D005260:Female; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology; D008414:Mastitis, Bovine / Q000276:immunology / Q000382:microbiology*; D052247:Nitric Oxide Synthase Type II / Q000276:immunology / Q000378:metabolism; D011388:Prolactin / Q000276:immunology* / Q000494:pharmacology; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000235:genetics / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology; D023083:beta-Defensins / Q000235:genetics / Q000276:immunology
D053583:Interleukin-1beta; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D023083:beta-Defensins; D011388:Prolactin; D052247:Nitric Oxide Synthase Type II