Enhanced tyrosine hydroxylase expression in PC12 cells co-cultured with feline mesenchymal stem cells.
Mesenchymal stem cells (MSCs) secrete a variety of neuroregulatory molecules, such as nerve growth factor, brain-derived neurotrophic factor, and glial cell-derived neurotrophic factor, which upregulate tyrosine hydroxylase (TH) gene expression in PC12 cells. Enhancing TH gene expression is a critical step for treatment of Parkinson's disease (PD). The objective of this study was to assess the effects of co-culturing PC12 cells with MSCs from feline bone marrow on TH protein expression. We divided the study into three groups: an MSC group, a PC12 cell group, and the combined MSC + PC12 cell group (the co-culture group). All cells were cultured in DMEM-HG medium supplemented with 10% fetal bovine serum for three days. Thereafter, the cells were examined using western blot analysis and immunocytochemistry. In western blots, the co-culture group demonstrated a stronger signal at 60 kDa than the PC12 cell group (p < 0.001). TH was not expressed in the MSC group, either in western blot or immunocytochemistry. Thus, the MSCs of feline bone marrow can up-regulate TH expression in PC12 cells. This implies a new role for MSCs in the neurodegenerative disease process.
Jin
Guang-Zhen
GZ
Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Korea.
Yin
Xi-Jun
XJ
Yu
Xian-Feng
XF
Cho
Su-Jin
SJ
Lee
Hyo-Sang
HS
Lee
Hyo-Jong
HJ
Kong
Il-Keun
IK
D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D015153:Blotting, Western; D002415:Cats / Q000502:physiology*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D015971:Gene Expression Regulation, Enzymologic; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000378:metabolism; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008858:Microscopy, Phase-Contrast; D016716:PC12 Cells / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D000954:Antigens, Surface; D014446:Tyrosine 3-Monooxygenase; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)