Detection of HIV-1 sequences in intestinal bacteria of HIV/AIDS patients.
OBJECTIVE
Bacterial DNA isolated from the intestinal tract of 11 American and 30 Slovak HIV/AIDS patients were analyzed by colony and dot blot hybridization assay for HIV-1 specific sequences. Secondly, PCR using primers specific for the HIV-1 gag, pol and env genes for detection of HIV-1 sequences in these DNA were performed.


RESULTS
Intestinal bacteria DNA of HIV/AIDS patients hybridized in colony and dot blot hybridization assay for HIV-1 specific sequences. PCR products synthesized on specific primers of HIV-1 gag (115 bp), env (142 bp), pol-env (1484) genes were found to be for more than 90% homologous to the corresponding sequence in HIV-1.


CONCLUSIONS
Intestinal bacteria of HIV/AIDS patients are bearing sequences for more than 90% identical with those of HIV-1.
Zajac
Vladimir
V
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovakia. vladimir.zajac@savba.sk
Stevurkova
Viola
V
Matelova
Lenka
L
Ujhazy
Eduard
E
D000163:Acquired Immunodeficiency Syndrome / Q000188:drug therapy / Q000821:virology; D044966:Anti-Retroviral Agents / Q000627:therapeutic use; D001483:Base Sequence; D016971:Citrobacter freundii / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D021902:Enterobacter aerogenes / Q000235:genetics; D004755:Enterobacteriaceae / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D015752:Genes, env / Q000235:genetics; D015750:Genes, gag / Q000235:genetics; D015751:Genes, pol / Q000235:genetics; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology; D015497:HIV-1 / Q000235:genetics*; D006716:Homosexuality; D006801:Humans; D007422:Intestines / Q000382:microbiology* / Q000821:virology; D008297:Male; D008969:Molecular Sequence Data; D011513:Proteus mirabilis / Q000235:genetics; D012367:RNA, Viral / Q000302:isolation & purification; D012016:Reference Values; D013210:Staphylococcus / Q000235:genetics
D044966:Anti-Retroviral Agents; D004269:DNA, Bacterial; D012367:RNA, Viral