[Determination of three alkaloids in total alkaloids exfracts from Lotus plumule by RP-HPLC].
OBJECTIVE
To develop a RP-HPLC method for determination of three alkaloids in total alkaloids exfracted from Lotus plumule.


METHODS
A Hypersil C18 column was used with the mobile phase of methanol-phosphate (73:27, pH 9.0) at the detection wavelength of 230 and 286 nm. The flow rate was 1 mL min(-1).


RESULTS
The linear range was 20-700 microg mL(-1), the recovery of three alkaloids were 97.9%, 98.4% and 98.1%. The RSD of intra-day and inter-day was 0. 24% -4. 83%. Different groups of samples were determined by this method. The result showed that the extraction method with methanol was prior to the extraction method with alcohol.


CONCLUSIONS
The method is simple, rapid and suitable for the determination of three alkaloids in total alkaloids exfracted from L. plumule.
Zhao
Li-bo
LB
Department of Pharmacology, Tongji Medical College, Huangzhong University of Science and Technology, Wuhan 430030, China.
Jin
You
Y
Wang
Jia-ling
JL
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D044182:Benzylisoquinolines / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D007546:Isoquinolines / Q000032:analysis*; D000070116:Lotus / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry
D000470:Alkaloids; D044182:Benzylisoquinolines; D004365:Drugs, Chinese Herbal; D007546:Isoquinolines; D010636:Phenols; C499779:isoliensinine; C057222:neferine; C080095:liensinine