[Establishment of a diagnostic model of serum protein fingerprint pattern for esophageal cancer screening in high incidence area and its clinical value].
OBJECTIVE
To analyze the alterations of serum proteomic pattern in esophageal squamous cell carcinoma (ESCC) by SELDI-TOF-MS, to establish a diagnostic model of ESCC screening in high incidence area and investigate its clinical value.


METHODS
SELDI-TOF-MS and CM10 proteinChip were used to detect the serum proteomic patterns of 36 cases of ESCC and 38 healthy control subjects in high incidence area. The data were analyzed and a diagnostic model was established by using support vector machine (SVM). The diagnostic model was evaluated by leave-one-out cross validation.


RESULTS
At the molecular weight range of 2000 to 20,000, 31 protein peaks were significantly different between ESCC and controls (P < 0.01). A diagnostic model consisting of 4 protein peaks could do the best in diagnosis of ESCC and controls. The accuracy was 85.1%, sensitivity was 86.1%, specificity was 84.2%, and positive value was 83.8%.


CONCLUSIONS
The diagnostic model formed by 4 protein peaks, established in this study, can well distinguish ESCC from healthy subjects. It provides a new approach for ESCC screening in high incidence area.
Wang
Shi-Jie
SJ
Department of Endoscopy, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China. Tim32@126.com
Zhang
Li-Wei
LW
Yu
Wei-Fang
WF
Yu
Jie-Kai
JK
Zheng
Shu
S
Li
Ying-Sai
YS
Er
Li-Mian
LM
Wen
Deng-Gui
DG
Gao
Jin-Hong
JH
D000328:Adult; D000368:Aged; D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D002681:China / Q000453:epidemiology; D004938:Esophageal Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D006801:Humans; D015994:Incidence; D008403:Mass Screening; D008875:Middle Aged; D010449:Peptide Mapping; D040081:Protein Array Analysis; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001798:Blood Proteins