title: Characterisation of Mycoplasma capricolum P60 surface lipoprotein and its evaluation in a recombinant ELISA.
abstract: This paper reports the identification and characterisation of a 60kDa surface protein antigen (P60) of Mycoplasma capricolum subspecies capricolum (Mcc), and describes its diagnostic application. Genomic localization and presence in P60 of conserved functional domains suggested a structural and functional relationship with the immunodominant antigen P48 of Mycoplasma agalactiae, a basic membrane protein. A rP60-ELISA was developed, and it resulted in a high specificity for Mcc infections after evaluation with 125 goat sera. The comparison with an existent ELISA based on whole Mcc cell lysates showed that the two assays have comparable sensitivities, but the rP60-ELISA has the significant advantage of a greater specificity. Results indicate that P60 is a potential marker of Mcc infection, especially useful in areas where the presence of M. capricolum subspecies capripenumoniae is also reported.
authors.affiliation: Dipartimento di Patologia e Clinica Veterinaria, Sezione di Malattie Infettive, Università degli Studi di Sassari, Sassari, Italy. alberti@uniss.it
authors.forename: Alberto
authors.initials: A
authors.lastname: Alberti
authors.forename: Patrizia
authors.initials: P
authors.lastname: Robino
authors.forename: Bernardo
authors.initials: B
authors.lastname: Chessa
authors.forename: Sergio
authors.initials: S
authors.lastname: Rosati
authors.forename: Maria Filippa
authors.initials: MF
authors.lastname: Addis
authors.forename: Pascale
authors.initials: P
authors.lastname: Mercier
authors.forename: Alessandro
authors.initials: A
authors.lastname: Mannelli
authors.forename: Tiziana
authors.initials: T
authors.lastname: Cubeddu
authors.forename: Margherita
authors.initials: M
authors.lastname: Profiti
authors.forename: Ennio
authors.initials: E
authors.lastname: Bandino
authors.forename: Richard
authors.initials: R
authors.lastname: Thiery
authors.forename: Marco
authors.initials: M
authors.lastname: Pittau
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D001483:Base Sequence; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D015511:Goat Diseases / Q000175:diagnosis*; D006041:Goats; D008074:Lipoproteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D045644:Mycoplasma agalactiae / Q000276:immunology; D045623:Mycoplasma capricolum / Q000235:genetics / Q000276:immunology*; D011002:Pleuropneumonia, Contagious / Q000175:diagnosis*; D017354:Point Mutation; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D008074:Lipoproteins; C116063:P60 protein, bacteria; D011993:Recombinant Fusion Proteins