title: [Immunolocalization of the antioxidant protein TPx of Echinococcus granulosus].
abstract: OBJECTIVE
  To study the localization of the antioxidant protein thioredoxin peroxidase (TPx) of Echinococcus granulosus (EgTPx) in the protoscoleces (PSC) of parasite.
  
  
  METHODS
  The protoscoleces of E.granulosus were aspirated and pooled from sheep liver hydatid cysts. After digested by pepsin, the sedimented protoscoleces were used for paraffin sections. The localization of the antioxidant protein TPx of EgTPx was determined by using(polyclonal antibody against rEgTPx) and indirect immunofluorescence staining.
  
  
  RESULTS
  Indirect immunofluorescence staining analysis indicated that the rEgTPx was mainly distributed in tegument, subtegument and calcareous corpuscle cells of the protoscolex.
  
  
  CONCLUSIONS
  The wide distribution and large sites of EgTPx in the parasite hare been clearly determined, which will help further investigation into the biological functions and application of TPx protein.
authors.affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
authors.forename: Qiu-Lian
authors.initials: QL
authors.lastname: Hou
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Zhuang-Zhi
authors.initials: ZZ
authors.lastname: Zhang
authors.forename: Wen-Yao
authors.initials: WY
authors.lastname: Cao
authors.forename: Fu-Chun
authors.initials: FC
authors.lastname: Zhang
authors.forename: Wen-Bao
authors.initials: WB
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D048209:Echinococcus granulosus / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D054464:Peroxiredoxins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D007106:Immune Sera; D054464:Peroxiredoxins