title: Potential use of blood bank platelet concentrates to accelerate wound healing of diabetic ulcers.
abstract: Many clinical trials have shown the effectiveness of platelet releasates on diabetic wound healing, but large volumes of blood must be aspirated from patients and a platelet separator is required. This study was undertaken to investigate the potential of blood bank platelet concentrate (BBPC) for accelerating diabetic wound healing. Platelet-derived growth factor-BB (PDGF-BB) contents in BBPC were determined by enzyme-linked immunosorbent assay in vitro, and the in vivo study involved comparing extents of wound healing in BBPC-treated and control groups using diabetic mouse wound models. In the in vitro study, 5.2 +/- 1.2 pg of PDGF-BB was found to be released by 1 million platelets in fresh BBPC, and adding thrombin to BBPC significantly increased the levels of PDGF-BB released. Our in vivo study in diabetic mice revealed that BBPC treatment greatly accelerated wound healing. Our results suggest that BBPC has potential to accelerate the healing of diabetic ulcers.
authors.affiliation: Department of Plastic Surgery, Korea University College of Medicine, Seoul, Korea. pshan@kumc.or.kr
authors.forename: Seung-Kyu
authors.initials: SK
authors.lastname: Han
authors.forename: Deok-Woo
authors.initials: DW
authors.lastname: Kim
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Jeong
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Hong
authors.forename: Hong-Suh
authors.initials: HS
authors.lastname: Woo
authors.forename: Woo-Kyung
authors.initials: WK
authors.lastname: Kim
authors.forename: Finn
authors.initials: F
authors.lastname: Gottrup
mesh_terms: D000818:Animals; D000077214:Becaplermin; D001771:Blood Banks; D001792:Blood Platelets / Q000378:metabolism*; D048909:Diabetes Complications / Q000628:therapy*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis; D012038:Regeneration; D013997:Time Factors; D014456:Ulcer / Q000628:therapy*; D014945:Wound Healing
chemical_list: D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin