Telomere DNA conformation change induced aggregation of gold nanoparticles as detected by plasmon resonance light scattering technique.
It has been reported that adsorption of uncoiled DNA (u-DNA) on the surface of gold nanoparticles (Au-NPs) can prevent the nanoparticle suspensions from aggregation even if in salt medium. Herein we report that quadruplex DNA (q-DNA), which is formed from uncoiled telomere DNA, via intramolecular hydrogen bonds in the presence of potassium ion, cannot keep Au-NPs stable, and the q-DNA/Au-NPs coexisting suspensions display aggregation tendency, giving plasmon resonance light scattering (PRLS) signals of Au-NPs. Mechanism investigations through a single point energy calculation on u- and q-structures of telomere DNA showed that q-DNA, compared with u-DNA, has a much higher surface negative charge density, symmetrical charge distribution and well self-structural stabilization, could not be adsorbed on the surface of Au-NPs.
Huang
Cheng Zhi
CZ
College of Pharmaceutical Sciences, CQKL-LRTA, Southwest University, Chongqing 400715, China. chengzhi@swu.edu.cn
Liao
Qie Gen
QG
Gan
Li Hua
LH
Guo
Feng Ling
FL
Li
Yuan Fang
YF
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D006046:Gold / Q000737:chemistry*; D008027:Light; D053768:Metal Nanoparticles; D009690:Nucleic Acid Conformation; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D020349:Surface Plasmon Resonance / Q000379:methods*; D016615:Telomere
D015342:DNA Probes; D006046:Gold; D004247:DNA