title: Mathematical model of outer hair cell regulation including ion transport and cell motility.
abstract: To understand the regulatory processes within the cochlea, and outer hair cells (OHCs) in particular, we have developed a mathematical model of OHC regulation that takes into account their known electrical properties, and includes fast and slow somatic motility. We model how cytosolic Ca(2+) is involved in regulation of (i) the OHC membrane potential, (ii) the operating point of OHC mechano-electrical transduction (MET) channels via slow motility; (iii) basolateral wall K(+) permeability via Ca(2+)-sensitive K(+) channels; and (iv) cytosolic concentrations of Ca(2+) itself, via Ca(2+)-ATPase-mediated sequestration within the OHCs and Ca(2+)-induced Ca(2+)-release (CICR) from the same intracellular Ca(2+) storage organelles. To account for some aspects of the cochlea's transient response to experimental perturbations, we have included a putative intracellular second-messenger cascade based on cytosolic Ca(2+). Overall, the OHC basolateral permeability determines the resting membrane potential of the OHCs and their standing current, which influences the endocochlear potential, and also affects the AC receptor potential that drives the prestin-mediated somatic electromotility and active cochlear gain. The model we have developed provides a physiologically-plausible and internally-consistent explanation for the time-courses of the cochlear changes we have observed during a number of different experimental perturbations, including a slow oscillatory behaviour presumed due to oscillations in cytosolic Ca(2+) concentration. We also show how the known ionic mechanisms within OHCs act to regulate membrane potential and hair bundle angle over a very wide range of strial current and intracochlear hydrostatic pressure. Not included in the model are osmotic effects, the nonlinear aspects of prestin's electromotility, the intracellular role of Cl(-) in modifying this motility, nor adaptation of MET at the apex of OHCs. Only one Ca(2+) sequestration compartment has been included in this implementation of the model, with the two types of basolateral Ca(2+) cisternae combined into a single compartment. Despite these limitations, the model as presented offers insights into the regulation of OHC membrane potential and MET at the hair cell apex, and is our first step in understanding in a quantitative way the integrated function of the molecular components of ion transport and motility in these cells.
authors.affiliation: The Auditory Laboratory, Discipline of Physiology, The University of Western Australia, Crawley, Australia. gregory.obeirne@canterbury.ac.nz
authors.forename: Greg A
authors.initials: GA
authors.lastname: O'Beirne
authors.forename: Robert B
authors.initials: RB
authors.lastname: Patuzzi
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002465:Cell Movement; D025461:Feedback, Physiological; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000378:metabolism*; D006874:Hydrostatic Pressure; D017136:Ion Transport; D008564:Membrane Potentials; D008954:Models, Biological; D011188:Potassium / Q000378:metabolism; D024681:Potassium Channels, Calcium-Activated / Q000378:metabolism; D015203:Reproducibility of Results; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D024681:Potassium Channels, Calcium-Activated; D000252:Calcium-Transporting ATPases; D011188:Potassium; D002118:Calcium