[Establishment and optimization of detection of the hepatitis B immune complexes].
OBJECTIVE
To establish and optimize methods to detect the immune complexes (IC) of hepatitis B virus directly.


METHODS
A C1q solid phase ELISA, mouse anti-HBs MAb solid phase ELISA and the complement consumption assay were established to detect the IC and these methods were optimized.


RESULTS
All the three methods were highly sensitive, specific and reproducible. The C1q used for coating tended to lose its activity easily at room temperature. Although strict requirements are needed for the raw and processed materials for complement consumption assay and the process of manipulation is complex, it can quantitatively detect IC. Comparing to the C1q solid ELISA and complement consumption assay, the mouse anti-HBs MAb solid phase ELISA has its own merits: convenience and stability.


CONCLUSIONS
Mouse anti-HBs MAb solid phase ELISA is the best way to detect IC directly.
Xu
Li-Feng
LF
National Vaccine and Serum Institute, Beijing, China.
Wang
Juan
J
Xu
Jing
J
Shi
Nan
N
Wu
Gang
G
D015922:Complement C1q / Q000032:analysis*; D003168:Complement Fixation Tests / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006509:Hepatitis B / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results
D015922:Complement C1q