title: The vitronectin gene in rainbow trout: cloning, expression and phylogenetic analysis.
abstract: Vitronectin is a major cell adhesion glycoprotein that is found in plasma and the extracellular matrix. Vitronectin consists of an N-terminal somatomedin B domain and two hemopexin-like domains and controls functions including cell adhesion, migration, haemostasis and immune defence. In order to study the molecular evolution of the complement lytic pathway regulation, we have cloned and characterized the vitronectin gene from rainbow trout (Oncorhynchus mykiss). The deduced amino acid sequence of trout vitronectin exhibits 45%, 46%, 47% and 63% identity with human, chicken, Xenopus and zebrafish orthologs, respectively. The domain architecture of the trout vitronectin, consisting of a somatomedin B domain and two hemopexin-like domains, resembles that of mammalian vitronectins. Analysis of partial genomic clones shows that trout vitronectin gene exhibits the same exon-intron organization profile as the human ortholog gene. The trout vitronectin gene is probably present as a single copy in the trout genome, showing a differential expression pattern among tissues investigated.
authors.affiliation: Department of Biology, School of Medicine, University of Patras, Rion 26500, Patras, Greece.
authors.forename: Dimitra J
authors.initials: DJ
authors.lastname: Marioli
authors.forename: Ioannis K
authors.initials: IK
authors.lastname: Zarkadis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D023061:Gene Order; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D019096:Vitronectin / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D019096:Vitronectin