title: Molecularly imprinted polymer-based solid-phase extraction combined with molecularly imprinted polymer-based sensor for detection of uric acid.
abstract: The development of an easy-to-use, rapid, robust and inexpensive technique is required which can measure the basal concentration of uric acid (UA) lower than 1.0 x 10(-7)M ( approximately 0.017 mgL(-1)) in biological samples to attend the problem of hypouricemia. In the present work an artificial receptor for UA, silica gel-bonded molecularly imprinted polymer (MIP), was used as a sorbent for molecularly imprinted solid-phase extraction (MISPE) in column chromatography. The use of a sensor based on a MIP-modified hanging mercury drop electrode (HMDE), as reported from our laboratory, could estimate UA with detection limit as low as 0.024 mgL(-1) under the optimized conditions of differential pulse, cathodic stripping voltammetric (DPCSV) measurement. However, in the current investigation, with the use of the combination of MISPE followed by detection with a MIP-based HMDE sensor, the minimum detectable concentration could go down to 0.0008 mgL(-1) (RSD=0.63%, S/N=3). The same MIP receptor for both MISPE and the corresponding sensor was able to enhance the preconcentration of analyte substantially so as to attain the desired level of sensitivity; and that to without any interference (cross-reactivity) from other structurally related analogues including the major interferent like ascorbic acid prevalent in the aqueous environment of biological samples.
authors.affiliation: Analytical Division, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India. prasadbb_2000@yahoo.com
authors.forename: Bhim Bali
authors.initials: BB
authors.lastname: Prasad
authors.forename: Piyush Sindhu
authors.initials: PS
authors.lastname: Sharma
authors.forename: Dhana
authors.initials: D
authors.lastname: Lakshmi
mesh_terms: D006801:Humans; D054802:Molecular Imprinting; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D014527:Uric Acid / Q000032:analysis / Q000097:blood* / Q000737:chemistry
chemical_list: D011108:Polymers; D014527:Uric Acid; D058428:Silica Gel; D012822:Silicon Dioxide