Importance of size-to-charge ratio in construction of stable and uniform nanoscale RNA/dendrimer complexes.
Formation of RNA/dendrimer complexes between various RNA molecules and PAMAM dendrimers was studied using atomic force microscopy. Our results demonstrate that effective construction of stable nanoscale and uniform RNA/dendrimer complexes depends critically on the size of the RNA molecule, the dendrimer generation and the charge ratio between the dendrimer and the RNA. Larger RNA molecules, higher generations of dendrimers and larger dendrimer-to-RNA charge ratios lead to the formation of stable, uniform nanoscale RNA/dendrimer complexes. These findings provide new insights in developing dendrimer systems for RNA delivery.
Shen
Xin-Cheng
XC
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, P.R. China.
Zhou
Jiehua
J
Liu
Xiaoxuan
X
Wu
Jiangyu
J
Qu
Fanqi
F
Zhang
Zhi-Ling
ZL
Pang
Dai-Wen
DW
Quéléver
Gilles
G
Zhang
Cheng-Cai
CC
Peng
Ling
L
D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D036103:Nanotechnology; D010316:Particle Size; D011073:Polyamines / Q000737:chemistry; D012313:RNA / Q000737:chemistry* / Q000648:ultrastructure; D020871:RNA Stability; D034741:RNA, Small Interfering / Q000737:chemistry / Q000648:ultrastructure
D050091:Dendrimers; C104700:PAMAM Starburst; D011073:Polyamines; D034741:RNA, Small Interfering; D012313:RNA