title: Effects of specific antisense oligonucleotide inhibition of Fas expression on T cell apoptosis induced by Fas ligand.
abstract: To investigate the effect of specific antisense oligodeoxynucleotide (ASODN) inhibition of Fas expression on T cell apoptosis induced by hepatocarcinoma cells. Fas receptor (Fas) and Fas ligand (FasL) expressed by the hepatocarcinoma cell line HepG2.2.15 and Jurkat T cells were detected by flow cytometry (FCM) and the ability of FasL-inducing T cell apoptosis was tested by co-culture assay in vitro with HepG2.2.15 cells and Jurkat T cells. The Jurkat cells were transfected with Fas-ASODN using lipofectin, and the effects of Fas-ASODN on Fas mRNA level, Fas expression on T cells surface, and apoptosis were investigated by RT-PCR, FCM and co-culture assay, respectively. It was found that HepG2.2.15 cells expressing functional FasL could induce the apoptosis of Jurkat cells as demonstrated by co-culture assays. After the Jurkat cells were transfected with Fas ASODN, the level of Fas mRNA, the expression rate of Fas and the apoptotic rate induced by hepatocarcinoma cells were all decreased. As a conclusion, it is evident that hepatocarcinoma cells expressing FasL can induce apoptosis in Fas-expressing T cells, indicating that transfection of Fas ASODN can partially convert the immune inhibitory condition induced by hepatocarcinoma cells.
authors.affiliation: Institute of Immunology, Shandong University School of Medicine, Jinan, 250012, People's Republic of China. haitingmao@yahoo.com
authors.forename: Haiting
authors.initials: H
authors.lastname: Mao
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Jiao
authors.initials: J
authors.lastname: Zhang
authors.forename: Hongtao
authors.initials: H
authors.lastname: Gu
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Xianbin
authors.initials: X
authors.lastname: Zhou
authors.forename: Haipeng
authors.initials: H
authors.lastname: Yin
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Fengxiang
authors.initials: F
authors.lastname: Xie
authors.forename: Guosheng
authors.initials: G
authors.lastname: Jiang
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D053222:Fas Ligand Protein / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000502:physiology*; D014162:Transfection; D019014:fas Receptor / Q000037:antagonists & inhibitors* / Q000235:genetics
chemical_list: D053222:Fas Ligand Protein; D016376:Oligonucleotides, Antisense; D019014:fas Receptor