[Experimental study on effect of Bailing Cpsule on dendritic cells in mice].
OBJECTIVE
Comparing with the classic immuno-supressors, to probe in the in vitro effect of Cordyceps Sinensis (CS) on the differentiation, maturation and function of dentritic cells (DCs), and further to explore its mechanism.


METHODS
Mice myeloid DCs were cultured respectively with extraction of Bailing Capsule (CSE), a Chinese medical preparation made of CS, Rapamycin and Tacrolimus, and the effect of various drugs on phenotype of DCs was analyzed with flow cytometer. Then, using as the stimulator, the DCs cultured with different drugs were mixed and cultured with heterogenous lymphocytes for observing the stimulating capacity of DCs on cell proliferation.


RESULTS
CSE showed no in vitro effect on phenotype markers and co-stimulation molecules of DCs, the difference between CSE and Tacrolimus was insignificant, while Rapamycin could reduce the two parameters. CSE showed a marked suppressive effect on DCs in stimulating leucocyte proliferation in a dose-dependent manner.


CONCLUSIONS
CSE could affect the stimulating capacity of DCs on cell proliferation, which is probably by means of inhibiting the function of antigen presentating cells to block the presentation of extrinsic signal, and make the low immune response condition, thus to obtain the effect of immunosuppression.
Ma
Lin-lin
LL
Beijing Chaoyang Hospital Affiliated to Capital University of Medical Sciences, Beijing. linlinma@hotmail.com
Yang
Xiao-yong
XY
Gao
Ju-zhong
JZ
D000818:Animals; D002214:Capsules; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D032661:Cordyceps / Q000737:chemistry; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D002214:Capsules; D004365:Drugs, Chinese Herbal; D007166:Immunosuppressive Agents; C533614:bailing capsules