The expression of myoglobin and ROR2 protein in Dupuytren's disease.
BACKGROUND
Dupuytren's disease (DD) is a hand disease inherited as an autosomal dominant trait with variable penetrance, especially among populations of northern European ancestry. The etiology and pathophysiology of DD are not clear. The purpose of this study was to examine the gene expression profiles of palmar fascia of DD and healthy patients using microarray analysis to highlight the genes that might contribute to the pathogenesis of DD.


METHODS
Dupuytren contracture samples were taken from excised mature cords of DD patients during aponeurectomies. Control samples were collected from healthy hand trauma patients. Microarray analysis was performed with the Affymetrix HGU133A genome array (Affymetrix, Santa Clara, CA). Expression changes of selected proteins were confirmed at the protein level with Western and dot blotting or by immunohistochemistry.


RESULTS
At least an 8-fold change in gene expression was found with 127 genes, including a 90-fold down-regulation of myoglobin and a 14-fold up-regulation of tyrosine kinase-like orphan receptor 2 (= ROR2) from absent to present during the disease. The changes in myoglobin and ROR2 expression were confirmed at the protein level.


CONCLUSIONS
In this study, we showed for the first time the connection of ROR2 in Dupuytren's disease. ROR2 and myoglobin may play an important role in the pathophysiology of this disease.
Forsman
Minna
M
Department of Surgery, Oulu University, Oulu, Finland. minna.forsman@ppshp.fi
Pääkkönen
Virve
V
Tjäderhane
Leo
L
Vuoristo
Jussi
J
Kallioinen
Leena
L
Salo
Tuula
T
Kallioinen
Matti
M
Ryhänen
Jorma
J
D000328:Adult; D000368:Aged; D026481:Blotting, Far-Western; D004387:Dupuytren Contracture / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D020022:Genetic Predisposition to Disease; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009211:Myoglobin / Q000096:biosynthesis*; D057050:Receptor Tyrosine Kinase-like Orphan Receptors; D011956:Receptors, Cell Surface / Q000096:biosynthesis*
D009211:Myoglobin; D011956:Receptors, Cell Surface; C078717:ROR2 protein, human; D057050:Receptor Tyrosine Kinase-like Orphan Receptors