title: Endothelium dysfunction caused by acute pressure distension of human saphenous vein used for myocardial revascularization.
abstract: OBJECTIVE
  To study morphofunctional alterations induced by brief pressure increases in human saphenous veins utilized in coronary artery bypass grafting.
  
  
  METHODS
  Saphenous veins of 20 patients undergoing coronary artery bypass grafting, were distributed into four experimental groups, control, 100 mmHg, 200 mmHg and 300 mmHg, and submitted to pressure distention over 15 seconds using Krebs solution. The evaluation included CD34 immunohistochemistry and an In vitro vascular reactivity study in organ chambers.
  
  
  RESULTS
  The main experimental findings were 1) From pressures of 200 mmHg there was a tendency to reduce the CD34 expression which became statistically significant at 300 mmHg; 2) There was no impairment of the contraction and relaxation as evidenced by in vitro vascular reactivity tests.
  
  
  CONCLUSIONS
  Although vascular reactivity impairment was not demonstrated in vitro, the CD34 expression, measured by immunohistochemistry, shows there is endothelium dysfunction at pressures of 300 mmHg.
authors.affiliation: Function Endothelial Laboratory of the Experimental Surgery Section in the Surgery and Anatomy, Department of the Medicine School in Ribeirão Preto, University ofSão Paulo.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Viaro
authors.forename: Carlos Gilberto
authors.initials: CG
authors.lastname: Carlotti
authors.forename: Alfredo José
authors.initials: AJ
authors.lastname: Rodrigues
authors.forename: Walter Vilella de Andrade
authors.initials: WV
authors.lastname: Vicente
authors.forename: Solange
authors.initials: S
authors.lastname: Bassetto
authors.forename: Graziela Saraiva
authors.initials: GS
authors.lastname: Reis
authors.forename: Lafaiete
authors.initials: L
authors.lastname: Alves
authors.forename: Paulo Roberto Barbosa
authors.initials: PR
authors.lastname: Evora
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000704:Analysis of Variance; D018952:Antigens, CD34 / Q000032:analysis* / Q000378:metabolism; D001794:Blood Pressure; D016022:Case-Control Studies; D001026:Coronary Artery Bypass / Q000379:methods / Q000592:standards; D003327:Coronary Disease / Q000601:surgery; D004730:Endothelium, Vascular / Q000187:drug effects / Q000503:physiopathology*; D005260:Female; D006801:Humans; D006874:Hydrostatic Pressure; D007150:Immunohistochemistry; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology; D012501:Saphenous Vein / Q000187:drug effects / Q000502:physiology* / Q000637:transplantation; D013314:Stress, Mechanical; D013718:Tensile Strength / Q000502:physiology; D020858:Tissue and Organ Harvesting; D014655:Vascular Resistance / Q000187:drug effects / Q000502:physiology*; D014661:Vasoconstriction / Q000187:drug effects / Q000502:physiology*; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D018952:Antigens, CD34; D014665:Vasodilator Agents; D000244:Adenosine Diphosphate