title: A novel lectin isolated from the hemolymph of the marine hair crab Erimacrus isenbeckii.
abstract: A lectin that induces hemagglutination activity in mouse and rabbit erythrocytes has been purified from the hemolymph of the marine hair crab Erimacrus isenbeckii. The results of SDS-PAGE, gel-filtration, affinity and anion-exchange chromatography indicate that this lectin, designated EIL (E. isenbeckii lectin), was successfully purified as a single protein, and comprises a mixture of a major (90%) dimeric and a minor (10%) oligomeric protein with a molecular mass of 116 kDa, with covalent linking between two subunits of 62 and 54 kDa. The activity was maximal at pH 5.6-8.0 and at temperatures below 50 degrees C. The N-terminal amino acid sequences were determined, and these differed greatly from those of other reported lectins from invertebrates, vertebrates, or plants. EIL binds with high specificities to both the O-acetylsialic acid and mannose that are present in bacterial pathogens, which suggests that EIL can act as a defense protein against infection in this crab.
authors.affiliation: Physical Pharmacy Laboratory, College of Pharmacy, Chung-Ang University, 221 Huksuk-Dong, Dongjak-Ku, Seoul, Korea.
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Na
authors.forename: Yu Jeong
authors.initials: YJ
authors.lastname: Kim
authors.forename: Byung Tae
authors.initials: BT
authors.lastname: Park
authors.forename: Byung Wook
authors.initials: BW
authors.lastname: Jung
authors.forename: Kwang Woo
authors.initials: KW
authors.lastname: Hwang
authors.forename: HaHyung
authors.initials: H
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003386:Brachyura; D004591:Electrophoresis, Polyacrylamide Gel; D006384:Hemagglutination / Q000187:drug effects; D006458:Hemolymph / Q000737:chemistry*; D037102:Lectins / Q000302:isolation & purification*; D008358:Mannose / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012260:Ribonucleases / Q000037:antagonists & inhibitors
chemical_list: D037102:Lectins; D012260:Ribonucleases; C029600:ribonuclease B; D008358:Mannose