Global transcriptome analysis of Borrelia burgdorferi during association with human neuroglial cells.
As adherence and entry of a pathogen into a host cell are key components to an infection, identifying the molecular mechanisms responsible for cellular association will provide a better understanding of a microbe's pathogenesis. We previously established an in vitro model for Borrelia burgdorferi infection of human neuroglial cells. To expand on our earlier study, we performed B. burgdorferi whole-genome expression analysis following a 20-hour infection of human neuroglial cells to identify borrelial genes that were differentially regulated during host-cell association compared with cultured Borrelia in cell-free medium. This study identifies several regulated genes, the products of which may be important mediators of cellular pathogenesis.
Livengood
Jill A
JA
National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Division of Vector-Borne Infectious Diseases, Bacterial Diseases Branch, Centers for Disease Control and Prevention, Fort Collins, Colorado 80521, USA.
Schmit
Virginia L
VL
Gilmore
Robert D
RD
D025065:Borrelia burgdorferi / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D005810:Multigene Family; D009457:Neuroglia / Q000166:cytology / Q000382:microbiology*; D016366:Open Reading Frames