title: Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration.
abstract: We made a three-dimensional (3-D) nanofibrous fibroin scaffold (NFS) with high porosity (94%) and examined its feasibility in bone regeneration. Under scanning electron microscopy, MC3T3-E1 preosteoblasts on the scaffold showed more spread on the first day after seeding compared with a 2-D scaffold. MTT assay showed significantly increased proliferation in 3-D NFS compared with 2-D NFS 7 days after seeding (P < 0.05). Western immunoblotting for activated paxillin, FAK, AKT, C-Src, and ERK1/2 antibodies showed signals from the extracellular matrix were significantly increased in 3-D NFS. Newly developed 3-D electrospun NFS may be a good candidate for use in bone regeneration.
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Chang Seok
authors.initials: CS
authors.lastname: Ki
authors.forename: Sook Young
authors.initials: SY
authors.lastname: Park
authors.forename: Hyun Jeong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Hong-Moon
authors.initials: HM
authors.lastname: Jung
authors.forename: Kyung Mi
authors.initials: KM
authors.lastname: Woo
authors.forename: Jung Weon
authors.initials: JW
authors.lastname: Lee
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Park
mesh_terms: D001672:Biocompatible Materials; D001861:Bone Regeneration; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D005349:Fibroins / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D010012:Osteogenesis; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D005349:Fibroins