Gene expression profiles and functional characterization of human immortalized endometriotic epithelial and stromal cells.
OBJECTIVE
To determine whether immortalized endometriotic cells can be used as potential models to unravel pathophysiology/pathogenesis of endometriosis in humans.


METHODS
In vitro study. Human immortalized endometriotic epithelial and stromal cells.


METHODS
College of Veterinary Medicine and Biomedical Sciences, Texas A&M University.


METHODS
Not applicable.


METHODS
None.


METHODS
Gene expression pattern and prostaglandin E(2) production and activity of matrix metalloproteinase 2 and matrix metalloproteinase 9.


RESULTS
Several genes associated with estrogen and progesterone biosynthesis and signaling, cell cycle regulation, extracellular matrix degradation, angiogenesis, cell growth and survival, cytokine production, and prostaglandin E(2) biosynthesis, transport, and signaling were expressed in human immortalized endometriotic cells. These endometriotic epithelial and stromal cells exhibited migrating and invading potential, produced a high quantity of prostaglandin E(2), and showed higher matrix metalloproteinase 2 and matrix metalloproteinase 9 activity compared to normal endometrial, epithelial, and stromal cells.


CONCLUSIONS
Human immortalized endometriotic epithelial and stromal cells could be used as ideal models to study the molecular and cellular aspects of endometriosis in humans.
Banu
Sakhila K
SK
Department of Integrative Biosciences, Reproductive Endocrinology and Cell Signaling Laboratory, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas 77843, USA.
Lee
JeHoon
J
Starzinski-Powitz
Anna
A
Arosh
Joe A
JA
D018797:Cell Cycle Proteins / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism
D018797:Cell Cycle Proteins; D016207:Cytokines; D004967:Estrogens; D011374:Progesterone; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9