title: Molecular epidemiology of rabies virus isolated from different sources during a bat-transmitted human outbreak occurring in Augusto Correa municipality, Brazilian Amazon.
abstract: We genetically characterize rabies virus (RABV) strains isolated from human cases, domestic and wild animals during a human outbreak of bat-transmitted rabies in Augusto Correa municipality, Pará state, Brazilian Amazon in 2005. Partial nucleotide sequences of the N gene (491 bp) were obtained for all strains, and phylogenetic analysis grouped these into two major clades (Pará and Central-Southeast) and identified them as bat-related viruses genotype I, Desmodus rotundus antigenic variant 3 (AgV3). A molecular clock was used to estimate the time of emergence for each RABV isolate. The molecular data from this study suggest the association of vampire bats with human and domestic animal cases reported in the outbreak, the circulation of at least two predominant lineages in the Pará state, and also a geographic association to lineages dispersion.
authors.affiliation: Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Belém, Brazil.
authors.forename: Taciana Fernandes Souza
authors.initials: TF
authors.lastname: Barbosa
authors.forename: Daniele Barbosa de Almeida
authors.initials: DB
authors.lastname: Medeiros
authors.forename: Elizabeth Salbe
authors.initials: ES
authors.lastname: Travassos da Rosa
authors.forename: Lívia Medeiros Neves
authors.initials: LM
authors.lastname: Casseb
authors.forename: Rita
authors.initials: R
authors.lastname: Medeiros
authors.forename: Armando de Souza
authors.initials: Ade S
authors.lastname: Pereira
authors.forename: Antonio Carlos Rosário
authors.initials: AC
authors.lastname: Vallinoto
authors.forename: Marcelo
authors.initials: M
authors.lastname: Vallinoto
authors.forename: Alberto Lopes
authors.initials: AL
authors.lastname: Begot
authors.forename: Reynaldo José da Silva
authors.initials: RJ
authors.lastname: Lima
authors.forename: Pedro Fernando da Costa
authors.initials: PF
authors.lastname: Vasconcelos
authors.forename: Márcio RobertoTeixeira
authors.initials: MR
authors.lastname: Nunes
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002685:Chiroptera / Q000821:virology*; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004196:Disease Outbreaks; D004199:Disease Vectors; D005260:Female; D005814:Genes, Viral; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D019590:Nucleocapsid Proteins / Q000235:genetics; D010802:Phylogeny; D011818:Rabies / Q000453:epidemiology* / Q000635:transmission / Q000821:virology*; D011820:Rabies virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D019590:Nucleocapsid Proteins; C099601:nucleocapsid protein, Rabies virus