title: Expression of matrix metalloproteinases-2 and -9 and RECK during alveolar bone regeneration in rat.
abstract: MMPs are endopeptidases that play a pivotal role in ECM turnover. RECK is a single membrane-anchored MMP-regulator. Here, we evaluated the temporal and spatial expression of MMP-2, MMP-9, and RECK during alveolar bone regeneration. The maxillary central incisor of Wistar rats was extracted and the animals were killed at 1, 3, 7, 10, 14, 21, 28, and 42 days post-operatively (n = 3/period). The hemimaxillae were collected, demineralized and embedded in paraffin. Immunohistochemical analysis was performed by the immunoperoxidase technique with polyclonal antibodies. On day 1, polymorphonuclear cells in the blood clot presented mild immunolabeling for MMPs. During bone remodeling, osteoblasts facing new bone showed positive staining for gelatinases and RECK in all experimental periods. MMPs were also found in the connective tissue and endothelial cells. Our results show for the first time that inactive and/or active forms of MMP-2, MMP-9 and RECK are differentially expressed by osteogenic and connective cells during several events of alveolar bone regeneration. This may be important for the replacement of the blood clot by connective tissue, and in the formation, maturation and remodeling of new bone.
authors.affiliation: Department of Endodontics, Bauru Dental School, University of Sao Paulo, Bauru, SP, Brazil.
authors.forename: Thais
authors.initials: T
authors.lastname: Accorsi-Mendonça
authors.forename: Katiúcia Batista da Silva
authors.initials: KB
authors.lastname: Paiva
authors.forename: Willian Fernando
authors.initials: WF
authors.lastname: Zambuzzi
authors.forename: Tânia Mary
authors.initials: TM
authors.lastname: Cestari
authors.forename: Vanessa Soares
authors.initials: VS
authors.lastname: Lara
authors.forename: Mari Cleide
authors.initials: MC
authors.lastname: Sogayar
authors.forename: Rumio
authors.initials: R
authors.lastname: Taga
authors.forename: José Mauro
authors.initials: JM
authors.lastname: Granjeiro
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D058851:GPI-Linked Proteins; D007124:Immunoenzyme Techniques; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000378:metabolism*; D008437:Maxilla; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D025521:Tumor Suppressor Proteins
chemical_list: D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; C529735:Reck protein, rat; D025521:Tumor Suppressor Proteins; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; D020780:Matrix Metalloproteinase 9