Effects of recombinant human PLD2 on proliferation and apoptosis of HL-60 cells.
In the present study, we investigated the role of recombinant human phospholipase D2 (rhPLD2) on proliferation and apoptosis in human leukemia HL-60 cells which induced by camptothecin. Our research demonstrated that various concentrations of rhPLD2 inhibit the growth of HL-60 cells in a dose-dependent manner, and rhPLD2 plus camptothecin can produce a synergistic effect on growth inhibition of HL-60 cells in vitro. So, we conclude that rhPLD2 alone cannot induce apoptosis in HL-60 cells, but it can potentiate the apoptosis of HL-60 cells induced by camptothecin. Similarly, we show that both rhPLD2 and standard PLD were able to enhance camptothecin-induced apoptosis of HL-60 cells.
Zhu
Ling
L
Immunology Department and Center of Neuroscience, Fujian Medical University, No. 88 Jiaotong Road, Fuzhou, Fujian 350004, China. zhling62@pub6.fz.fj.cn
Wang
Ya-Xiong
YX
Yu
Chuan-Xing
CX
D017209:Apoptosis / Q000187:drug effects*; D002166:Camptothecin / Q000494:pharmacology; D002453:Cell Cycle; D049109:Cell Proliferation / Q000187:drug effects*; D048430:Cell Shape; D053938:DNA Fragmentation; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D010739:Phospholipase D / Q000235:genetics / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; C105606:phospholipase D2; D010739:Phospholipase D; D002166:Camptothecin