title: Effect of cevimeline on radiation-induced salivary gland dysfunction and AQP5 in submandibular gland in mice.
abstract: The aim of this study was to clarify the effects of the muscarinic receptor agonist, cevimeline, on saliva flow and expression of aquaporin5 (AQP5) in submandibular gland after X-ray irradiation. Using a previously established radiation-induced xerostomia model mouse, saliva flow from at 7 days before irradiation to at 28 days after irradiation was investigated in mice that were treated with cevimeline before or after irradiation. Radiation caused a significant decrease in saliva flow compared with nonirradiated salivary glands. Cevimeline post-treatment also caused a significant decrease in saliva flow. In contrast, cevimeline pre-treatment did not significantly decrease saliva flow. Expression of AQP5 fluorescent intensity and mRNA were also analyzed. Irradiation significantly decreased expression of AQP5 in submandibular gland. However, pre-treatment with cevimeline prevented this decrease in AQP5 expression. These data suggest that pretreatment with cevimeline prevents radiation-induced xerostomia and radiation-induced decrease in expression of AQP5 in submandibular gland.
authors.affiliation: Department of Oral Medicine, Oral and Maxillofacial Surgery, Tokyo Dental Collage, Ichikawa, Chiba, Japan.
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Takakura
authors.forename: Sachiko
authors.initials: S
authors.lastname: Takaki
authors.forename: Ienaka
authors.initials: I
authors.lastname: Takeda
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hanaue
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kizu
authors.forename: Morio
authors.initials: M
authors.lastname: Tonogi
authors.forename: Gen-yuki
authors.initials: GY
authors.lastname: Yamane
mesh_terms: D000818:Animals; D051402:Aquaporin 5 / Q000032:analysis / Q000187:drug effects* / Q000528:radiation effects; D004195:Disease Models, Animal; D005260:Female; D005455:Fluorescent Antibody Technique; D051379:Mice; D008813:Mice, Inbred ICR; D018721:Muscarinic Agonists / Q000627:therapeutic use*; D011812:Quinuclidines / Q000627:therapeutic use*; D011829:Radiation Dosage; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012463:Saliva / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D012636:Secretory Rate / Q000187:drug effects / Q000528:radiation effects; D013363:Submandibular Gland / Q000528:radiation effects*; D013364:Submandibular Gland Diseases / Q000188:drug therapy* / Q000209:etiology; D013876:Thiophenes / Q000627:therapeutic use*; D013997:Time Factors; D014965:X-Rays; D014987:Xerostomia / Q000188:drug therapy* / Q000209:etiology
chemical_list: C496038:Aqp5 protein, mouse; D051402:Aquaporin 5; D018721:Muscarinic Agonists; D011812:Quinuclidines; D013876:Thiophenes; C059240:cevimeline