title: In vivo growth inhibition of sarcoma 180 by piperlonguminine, an alkaloid amide from the Piper species.
abstract: Many authors have already emphasized that phytochemicals from spices have biological applications. Piperlonguminine is a known alkaloid amide from peppers, including Piper divaricatum. The aim of this study was to investigate the in vitro and in vivo antitumor effects of piperlonguminine in experimental models. In order to evaluate the toxicological aspects related to piperlonguminine treatment, hematological, biochemical, histopathological and morphological analyses of treated animals were performed. Piperlonguminine did not show any significant in vitro cytotoxic effect at experimental exposure levels, but showed an in vivo antitumor effect. After 7 days of treatment, the inhibition rates were 38.71% and 40.68% at doses of 25 mg kg(-1) and 50 mg kg(-1), respectively. The histopathological analysis suggests that the liver and kidney were only weakly affected by piperlonguminine treatment. Neither the enzymatic activity of transaminases (AST and ALT) nor the urea levels were significantly altered. In the hematological analysis, all parameters analysed remained constant after piperlonguminine treatment. In conclusion, these data reinforce the anticancer potential of spice components.
authors.affiliation: Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Caixa Postal 3157, 60430-270 Fortaleza, Ceará, Brazil.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Bezerra
authors.forename: Cláudia
authors.initials: C
authors.lastname: Pessoa
authors.forename: Manoel Odorico de
authors.initials: MO
authors.lastname: Moraes
authors.forename: Nylane M N de
authors.initials: NM
authors.lastname: Alencar
authors.forename: Rodney O
authors.initials: RO
authors.lastname: Mesquita
authors.forename: Michael W
authors.initials: MW
authors.lastname: Lima
authors.forename: Ana Paula N N
authors.initials: AP
authors.lastname: Alves
authors.forename: Otília Deusdênia L
authors.initials: OD
authors.lastname: Pessoa
authors.forename: João Henrique
authors.initials: JH
authors.lastname: Chaves
authors.forename: Edilberto R
authors.initials: ER
authors.lastname: Silveira
authors.forename: Letícia V
authors.initials: LV
authors.lastname: Costa-Lotufo
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use*; D001772:Blood Cell Count; D001835:Body Weight / Q000187:drug effects; D045744:Cell Line, Tumor; D004148:Dioxolanes / Q000627:therapeutic use*; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D009929:Organ Size / Q000187:drug effects; D031701:Piper / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000473:pathology; D010881:Piperidones
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004148:Dioxolanes; C498077:piperlongumine; D010881:Piperidones