title: Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets.
abstract: The response of rainbow trout (Oncorhynchus mykiss, Walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (APR). Proteomic analysis by two-dimensional electrophoresis (2D) concurrently with mass spectrometry was used to detect APR related proteins in rainbow trout serum following feeding with probiotics Aeromonas sobria GC2 and Bacillus sp. JB-1. Three candidate proteins increased following use of GC2, and were putatively identified as NADH dehydrogenase, dystrophin and mKIAA0350. Conversely, one of the proteins, which were induced following use of JB-1 was identified as transferrin.
authors.affiliation: School of Life Sciences, John Muir Building, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, Scotland, UK.
authors.forename: Jason
authors.initials: J
authors.lastname: Brunt
authors.forename: Rasmus
authors.initials: R
authors.lastname: Hansen
authors.forename: Derek J
authors.initials: DJ
authors.lastname: Jamieson
authors.forename: Brian
authors.initials: B
authors.lastname: Austin
mesh_terms: D000210:Acute-Phase Reaction / Q000097:blood* / Q000276:immunology; D000333:Aeromonas / Q000276:immunology; D000818:Animals; D017756:Aquaculture / Q000379:methods; D001407:Bacillus / Q000276:immunology; D016189:Dystrophin / Q000097:blood; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D005393:Fish Diseases / Q000097:blood* / Q000276:immunology / Q000517:prevention & control; D009245:NADH Dehydrogenase / Q000097:blood; D017686:Oncorhynchus mykiss / Q000097:blood* / Q000276:immunology / Q000378:metabolism; D019936:Probiotics / Q000494:pharmacology*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary; D014168:Transferrin / Q000378:metabolism
chemical_list: D016189:Dystrophin; D014168:Transferrin; D009245:NADH Dehydrogenase