title: The isolation of Corynebacterium coyleae from clinical samples: clinical and microbiological data.
abstract: Fourteen Corynebacterium coyleae isolates were recovered from 12 in-patients during a 5-years period. In six patients, the isolates were considered as clinically significant, three definite (sepsis), two probable (sepsis and soft tissue infection), and one possible (post-transfusional bacteremia). In the remaining 6 patients (all neonatal bacteremias), there was not enough data for considering the isolates as clinical significant. API Coryne identified all isolates as C. jeikeium, while Biolog GP2 correctly identified 7 out of the 14 isolates. Definitive identification was achieved in all isolates by the sequencing of a fragment of 724 to 1423 pb of 16S rDNA. Successive isolations from two patients presented identical random amplified polymorphic DNA (RAPD) profiles. All of the isolates were in-vitro-sensitive to beta-lactams, gentamicin, rifampin, tetracycline, vancomycin, linezolid, and resistant to clindamycin. Resistance to erythromycin occurred in 83.3% of isolates, all of them presenting phenotype cMLS and harboring the gene ermX.
authors.affiliation: Department of Clinical Microbiology, Complejo Asistencial de León, Sacyl, Altos de Nava s/n, 24071 León, Spain. ifnatal@ono.com
authors.forename: M I
authors.initials: MI
authors.lastname: Fernández-Natal
authors.forename: J A
authors.initials: JA
authors.lastname: Sáez-Nieto
authors.forename: R
authors.initials: R
authors.lastname: Fernández-Roblas
authors.forename: M
authors.initials: M
authors.lastname: Asencio
authors.forename: S
authors.initials: S
authors.lastname: Valdezate
authors.forename: S
authors.initials: S
authors.lastname: Lapeña
authors.forename: R H
authors.initials: RH
authors.lastname: Rodríguez-Pollán
authors.forename: J M
authors.initials: JM
authors.lastname: Guerra
authors.forename: J
authors.initials: J
authors.lastname: Blanco
authors.forename: F
authors.initials: F
authors.lastname: Cachón
authors.forename: F
authors.initials: F
authors.lastname: Soriano
mesh_terms: D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D003352:Corynebacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D003354:Corynebacterium Infections / Q000382:microbiology* / Q000503:physiopathology*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008826:Microbial Sensitivity Tests; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D018805:Sepsis / Q000382:microbiology; D017422:Sequence Analysis, DNA; D018461:Soft Tissue Infections / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S