A novel cis-acting element required for DNA damage-inducible expression of yeast DIN7.
Din7 is a DNA damage-inducible mitochondrial nuclease that modulates the stability of mitochondrial DNA (mtDNA) in Saccharomyces cerevisiae. How DIN7 gene expression is regulated, however, has remained largely unclear. Using promoter sequence alignment, we found a highly conserved 19-bp sequence in the promoter regions of DIN7 and NTG1, which encodes an oxidative stress-inducible base-excision-repair enzyme. Deletion of the 19-bp sequence markedly reduced the hydroxyurea (HU)-enhanced DIN7 promoter activity. In addition, nuclear fractions prepared from HU-treated cells were used in in vitro band shift assays to reveal the presence of currently unidentified trans-acting factor(s) that preferentially bound to the 19-bp region. These results suggest that the 19-bp sequence is a novel cis-acting element that is required for the regulation of DIN7 expression in response to HU-induced DNA damage.
Yoshitani
Ayako
A
Chemical Genetics Laboratory, RIKEN Discovery Research Institute, Hirosawa 2-1, Wako-shi, Saitama 351-0198, Japan.
Yoshida
Minoru
M
Ling
Feng
F
D001483:Base Sequence; D017124:Conserved Sequence; D004249:DNA Damage / Q000235:genetics*; D004271:DNA, Fungal / Q000737:chemistry; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005090:Exodeoxyribonucleases / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D006918:Hydroxyurea / Q000494:pharmacology; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000235:genetics; D011401:Promoter Regions, Genetic; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism
D004271:DNA, Fungal; D004791:Enzyme Inhibitors; D029701:Saccharomyces cerevisiae Proteins; C105071:DIN7 protein, S cerevisiae; D005090:Exodeoxyribonucleases; D009699:N-Glycosyl Hydrolases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; C101825:NTG1 protein, S cerevisiae; D006918:Hydroxyurea