Biochemical composition and immunological comparison of select pecan [Carya illinoinensis (Wangenh.) K. Koch] cultivars.
On an edible portion basis, pecan moisture, protein, lipid, total soluble sugars, and ash contents ranged from 2.1% to 6.4%, 6.0% to 11.3%, 65.9% to 78.0%, 3.3% to 5.3%, and 1.2% to 1.8%, respectively. With the exception of a high tannin (2.7%) Texas seedling, pecan tannin content was in a narrow range (0.6-1.85%). Unsaturated fatty acids (>90%) dominated pecan lipid composition with oleic (52.52-74.09%) and linoleic (17.69-37.52%) acids as the predominant unsaturated fatty acids. Location significantly influenced pecan biochemical composition. Pecan lipid content was negatively correlated with protein (r = -0.663) and total sugar (r = -0.625). Among the samples tested using SDS-PAGE a common pattern, with minor differences, in subunit polypeptide profiles was revealed. Rabbit polyclonal antibody-based immunoblotting experiments (Western blot) also illustrated the similarity in polypeptide profiles with respect to immunoreactivity. All tested cultivars registered similar immunoreactivity when their protein extracts (each at 1 mg/mL) were assessed using inhibition ELISAs (mean +/- standard deviation = 0.89 +/- 0.20; n = 27) with the USDA "Desirable" cultivar as the reference standard (immunoreactivity designated as 1.0).
Venkatachalam
Mahesh
M
Department of Nutrition, Food, and Exercise Sciences, College of Human Sciences, Florida State University, Tallahassee, Florida 32306, USA.
Kshirsagar
Harshal H
HH
Seeram
Navindra P
NP
Heber
David
D
Thompson
Tommy E
TE
Roux
Kenneth H
KH
Sathe
Shridhar K
SK
D000906:Antibodies / Q000276:immunology; D002241:Carbohydrates / Q000032:analysis; D031325:Carya / Q000737:chemistry* / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D005504:Food Analysis; D009584:Nitrogen / Q000032:analysis; D009754:Nuts / Q000737:chemistry*; D010940:Plant Proteins / Q000032:analysis; D013045:Species Specificity; D013634:Tannins / Q000032:analysis
D000906:Antibodies; D002241:Carbohydrates; D005231:Fatty Acids, Unsaturated; D010940:Plant Proteins; D013634:Tannins; D009584:Nitrogen