title: PrPC interacts with tetraspanin-7 through bovine PrP154-182 containing alpha-helix 1.
abstract: The cellular prion protein (PrP(C)) is highly conserved in the evolution of mammals, and therefore, thought to have important cellular functions. Despite decades of intensive research, the physiological function of PrP(C) remains enigmatic. We carried out a yeast two-hybrid screen on a bovine brain cDNA expression library and identified the transmembrane protein tetraspanin-7 (CD231), as a PrP(C) interacting protein. We confirmed the interaction between PrP(C) and tetraspanin-7 by yeast two-hybrid assay, immunofluorescent co-localization, and immunocoprecipitation. Our mutational studies further demonstrated that PrP(C) specifically binds tetraspanin-7 through the region corresponding to bovine PrP(154-182) containing alpha-helix 1.
authors.affiliation: The State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Mingxiong
authors.initials: M
authors.lastname: Guo
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.forename: Yejian
authors.initials: Y
authors.lastname: Cui
authors.forename: Baiqun
authors.initials: B
authors.lastname: Pan
authors.forename: Ao
authors.initials: A
authors.lastname: Shen
authors.forename: Yuting
authors.initials: Y
authors.lastname: Sun
authors.forename: Yourong
authors.initials: Y
authors.lastname: Yi
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Gengfu
authors.initials: G
authors.lastname: Xiao
authors.forename: Guihong
authors.initials: G
authors.lastname: Sun
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D018076:DNA, Complementary / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D018621:PrPC Proteins / Q000737:chemistry* / Q000378:metabolism*; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D018076:DNA, Complementary; D008565:Membrane Proteins; D010446:Peptide Fragments; D018621:PrPC Proteins