title: Regulation of rtt107 recruitment to stalled DNA replication forks by the cullin rtt101 and the rtt109 acetyltransferase.
abstract: RTT107 (ESC4, YHR154W) encodes a BRCA1 C-terminal domain protein that is important for recovery from DNA damage during S phase. Rtt107 is a substrate of the checkpoint kinase Mec1, and it forms complexes with DNA repair enzymes, including the nuclease subunit Slx4, but the role of Rtt107 in the DNA damage response remains unclear. We find that Rtt107 interacts with chromatin when cells are treated with compounds that cause replication forks to arrest. This damage-dependent chromatin binding requires the acetyltransferase Rtt109, but it does not require acetylation of the known Rtt109 target, histone H3-K56. Chromatin binding of Rtt107 also requires the cullin Rtt101, which seems to play a direct role in Rtt107 recruitment, because the two proteins are found in complex with each other. Finally, we provide evidence that Rtt107 is bound at or near stalled replication forks in vivo. Together, these results indicate that Rtt109, Rtt101, and Rtt107, which genetic evidence suggests are functionally related, form a DNA damage response pathway that recruits Rtt107 complexes to damaged or stalled replication forks.
authors.affiliation: Department of Biochemistry and Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.
authors.forename: Tania M
authors.initials: TM
authors.lastname: Roberts
authors.forename: Iram Waris
authors.initials: IW
authors.lastname: Zaidi
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Vaisica
authors.forename: Matthias
authors.initials: M
authors.lastname: Peter
authors.forename: Grant W
authors.initials: GW
authors.lastname: Brown
mesh_terms: D000107:Acetylation / Q000187:drug effects; D002843:Chromatin / Q000378:metabolism; D044842:Cullin Proteins / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects; D017353:Gene Deletion; D051548:Histone Acetyltransferases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008954:Models, Biological; D009687:Nuclear Proteins / Q000378:metabolism*; D010641:Phenotype; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D018741:Replication Origin; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013489:Suppression, Genetic / Q000187:drug effects; D013696:Temperature
chemical_list: D002843:Chromatin; D044842:Cullin Proteins; D006657:Histones; D009687:Nuclear Proteins; C476451:RTT101 protein, S cerevisiae; C501265:RTT107 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D008741:Methyl Methanesulfonate; D051548:Histone Acetyltransferases; C517120:Rtt109 protein, S cerevisiae; D008239:Lysine