title: Evidence of brain mitochondrial activities after oxygen inhalation by 31P magnetic resonance spectroscopy at 3T.
abstract: The purpose of this study was to explore mitochondrial activities after oxygen inhalation by 31P magnetic resonance spectroscopy. Six healthy volunteers were studied using a GE 3T scanner. A spin-echo MRS sequence was utilized with a GE service coil. TR was 2000 msec and TE 35 msec with 128 scan averages. Before 31P scan, a 1H MRS pre-scan was performed to obtain shimming values. Exact two 31P scans were performed before and after oxygen inhalation through nasal cannula at a flow rate of 10 L/min. Data were processed offline using the SAGE/IDL software. Peak integral values of inorganic phosphor, phosphocreatine, and ATPs were measured. Peak integral values of inorganic phosphor were decreased from 1.44% to 16.62% (mean+/-SD, 8.2+/-4.60). Peak integral values of phosphocreatine were increased from 1.06% to 8.64% (4.54+/-2.74). Peak integral values of gammaATP were increased from 0.75% to 15.97% (5.94+/-6.10); alphaATP from 1.21% to 16.05% (7.04+/-6.14); betaATP from 1.01% to 7.12% (3.08+/-2.30). Brain mitochondrial activities were increased and more ATPs were produced after oxygen inhalation in healthy volunteers.
authors.affiliation: Department of Medical Imaging, Shantou University Medical College, China. rhwu@stu.edu.cn
authors.forename: Ren-Hua
authors.initials: RH
authors.lastname: Wu
authors.forename: Julien
authors.initials: J
authors.lastname: Poublanc
authors.forename: Danny
authors.initials: D
authors.lastname: Mandell
authors.forename: Garry
authors.initials: G
authors.lastname: Detzler
authors.forename: Adrian
authors.initials: A
authors.lastname: Crawley
authors.forename: Qing-Chun
authors.initials: QC
authors.lastname: Qiu
authors.forename: Karel
authors.initials: K
authors.lastname: terBrugge
authors.forename: David J
authors.initials: DJ
authors.lastname: Mikulis
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000280:Administration, Inhalation; D001921:Brain / Q000378:metabolism*; D004734:Energy Metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008928:Mitochondria / Q000378:metabolism*; D010100:Oxygen / Q000008:administration & dosage / Q000378:metabolism; D010101:Oxygen Consumption; D010725:Phosphocreatine / Q000031:analogs & derivatives / Q000378:metabolism; D010759:Phosphorus Isotopes
chemical_list: D010759:Phosphorus Isotopes; D010725:Phosphocreatine; C025403:phosphocreatinine; D000255:Adenosine Triphosphate; D010100:Oxygen