Molecular diversity of Brazilian strains of porcine circovirus type 2 (PCV-2).
Porcine circovirus 2 (PCV-2) is associated with a broad range of syndromes. In this study, eight pig tissue samples from two Brazilian states were analyzed using six PCR primer pairs amplifying a 1705-bp fragment of the PCV-2 genome. The NJ distance-based method was used for the phylogenetic analysis with the eight field strains herein, 15 GenBank sequences and using PCV-1 as an out-group. This yielded two major clusters (A and B) for this viral species, with the Brazilian strains segregating with European and Asian sequences. Nucleotide identity was 99.7 to 100% among the sequences. This information can be used in further studies of pathogenesis related to PCV-2 in Brazil.
de Castro
Alessandra Marnie Martins Gomes
AM
Faculdade de Jaguariúna, Curso de Medicina Veterinária, Rodovia Adhemar de Barros, Km 127, 13.820-000, Jaguariúna, São Paulo, Pista Sul, Brazil. alessandra@faj.br
Cortez
Adriana
A
Heinemann
Marcos Bryan
MB
Brandão
Paulo Eduardo
PE
Richtzenhain
Leonardo José
LJ
D000818:Animals; D001938:Brazil / Q000453:epidemiology; D018173:Circoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000145:classification* / Q000235:genetics*; D014644:Genetic Variation; D013552:Swine