title: [Study of HPLC-DAD fingerprint on complex traditional Chinese medicine proprietary preparation-Baoji pills].
abstract: OBJECTIVE
  Based on 'Back-tracking' method, identification and quality evaluation of complex traditional Chinese medicine (TCM) preparation of Baoji pills (BJP) were carried out by HPLC fingerprint analysis.
  
  
  METHODS
  HPLC-DAD fingerprint of BJP was conducted with Zorbax SB-C18 column and non-linear elution with the mobile phase consisted of acetonitrile-0.5% glacial acetic acid at column temperature 30 degrees C and detective wavelengths of 250 nm and 283 nm. From the established chromatographic pattern of BJP, track backward to the corresponding crude herbal drugs in the formula, attribution ofmost peaks in the BJP fingerprint can be disclosed.
  
  
  RESULTS
  The BJP HPLC fingerprint consisted of 44 peaks among which 35 peaks were assigned by parallel comparison with the fingerprint of the 10 corresponding crude drugs in the formula such as pueraria, pummelo peel, and magnolia bark, etc. and 22 peaks we reidentified by comparison with the chemical reference substances.
  
  
  CONCLUSIONS
  The established HPLC fingerprint represents the whole character of BJP, which enhanced the specialty for control and assessment of the product quality. It exemplified much more effective for quality control than selecting any marker for qualitative or quantitative testing target. And the Back-tracking' experimental method extended the study mentality for complex formula TCM products chromatographic fingerprinting analysis.
authors.affiliation: Zhuhai Chromap Institute of Herbal Medicine Research, Zhuhai 519085, China.
authors.forename: Xiang-hong
authors.initials: XH
authors.lastname: Wang
authors.forename: Pei-shan
authors.initials: PS
authors.lastname: Xie
authors.forename: Run-tao
authors.initials: RT
authors.lastname: Tian
authors.forename: Xiao-dan
authors.initials: XD
authors.lastname: Huang
authors.forename: Rong-bo
authors.initials: RB
authors.lastname: Zheng
authors.forename: Chun-mei
authors.initials: CM
authors.lastname: Qin
authors.forename: Qiong-xi
authors.initials: QX
authors.lastname: Yu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031202:Chrysanthemum / Q000737:chemistry; D002957:Citrus / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D031566:Magnolia / Q000737:chemistry; D008516:Medicine, Chinese Traditional; D010946:Plants, Medicinal / Q000737:chemistry*; D029908:Pueraria / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C532976:baoji