Yeast cell-surface expression of chitosanase from Paenibacillus fukuinensis.
To produce chitoorigosaccharides using chitosan, we attempted to construct Paenibacillus fukuinensis chitosanase-displaying yeast cells as a whole-cell biocatalyst through yeast cell-surface engineering. The localization of the chitosanase on the yeast cell surface was confirmed by immunofluorescence labeling of cells. The chitosanase activity of the constructed yeast was investigated by halo assay and the dinitrosalicylic acid method.
Fukuda
Takeshi
T
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Japan.
Isogawa
Danya
D
Takagi
Madoka
M
Kato-Murai
Michiko
M
Kimoto
Hisashi
H
Kusaoke
Hideo
H
Ueda
Mitsuyoshi
M
Suye
Shin-Ichiro
S
D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D001709:Biotechnology / Q000379:methods*; D002384:Catalysis; D002462:Cell Membrane / Q000201:enzymology*; D003001:Cloning, Molecular; D005818:Genetic Engineering; D006026:Glycoside Hydrolases / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D009844:Oligosaccharides / Q000096:biosynthesis; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D001426:Bacterial Proteins; D009844:Oligosaccharides; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C019868:chitosanase