Anti-inflammatory effects of moxifloxacin and human beta-defensin 2 association in human lung epithelial cell line (A549) stimulated with lipopolysaccharide.
Epithelia in the human airways, from the nasal aperture to the alveoli, are covered in a protective film of fluid containing a number of antimicrobial proteins. Defensins are single-chain, strongly cationic peptides and are one of the most extensively studied classes of antimicrobial peptides. Moxifloxacin (MXF) is a fluoroquinolone that acts against both Gram positive and Gram negative bacteria. In this study, we evaluated the effects of HBD2, MXF and the association MXF/HBD2 on some cytokines and on the ICAM-1 expression in LPS-stimulated A549 cells. Our results suggest that by lowering the epithelial cell-derived IL-1beta, IL-6, IL-8 and ICAM-1 expression, the MXF/HBD2 association interferes with the multifunctional cytokine network evolving during inflammatory processes of the respiratory tract; this anti-inflammatory potential could be of great value in the treatment of inflammatory disorders.
Donnarumma
Giovanna
G
Faculty of Medicine and Surgery, Second University of Naples, Department of Experimental Medicine, Microbiology and Clinical Microbiology, Via Costantinopoli 16, 80138 Naples, Italy.
Paoletti
Iole
I
Buommino
Elisabetta
E
Iovene
Maria Rosaria
MR
Tudisco
Laura
L
Cozza
Valentina
V
Tufano
Maria Antonietta
MA
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001372:Aza Compounds / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D024841:Fluoroquinolones; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism / Q000494:pharmacology; D008168:Lung / Q000166:cytology; D000077266:Moxifloxacin; D009504:Neutrophils / Q000187:drug effects; D011804:Quinolines / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D023083:beta-Defensins / Q000494:pharmacology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001372:Aza Compounds; C508606:CXCL8 protein, human; D016207:Cytokines; C106641:DEFB4A protein, human; D024841:Fluoroquinolones; C508600:IL6 protein, human; D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D011804:Quinolines; D014409:Tumor Necrosis Factor-alpha; D023083:beta-Defensins; D018799:Intercellular Adhesion Molecule-1; D000077266:Moxifloxacin