Cytotoxicity of Mycoplasma mycoides subsp. mycoides small colony type to bovine epithelial cells.
The cytotoxicities of various strains of Mycoplasma mycoides subsp. mycoides small colony type (SC), the agent of contagious bovine pleuropneumonia (CBPP), were measured in vitro using embryonic calf nasal epithelial (ECaNEp) cells. Strains isolated from acute cases of CBPP induced high cytotoxicity in the presence of glycerol, concomitant with the release of large amounts of toxic H2O2 that were found to be translocated into the cytoplasms of the host cells by close contact of the Mycoplasma strains with the host cells. Currently used vaccine strains also showed high cytotoxicity and high H2O2 release, indicating that they are attenuated in another virulence attribute. Strains isolated from recent European outbreaks of CBPP with mild clinical signs, which are characterized by a defect in the glycerol uptake system, released small amounts of H2O2 and showed low cytotoxicity to ECaNEp cells. M. mycoides subsp. mycoides SC strain PG1 released large amounts of H2O2 but was only slightly cytotoxic. PG1 was found to have a reduced capacity to bind to ECaNEp cells and was unable to translocate H2O2 into the bovine cells, in contrast to virulent strains that release large amounts of H2O2. Thus, an efficient translocation of H2O2 into host cells is a prerequisite for the cytotoxic effect and requires an intact adhesion mechanism to ensure a close contact between mycoplasmas and host cells.
Bischof
Daniela F
DF
Institute of Veterinary Bacteriology, Vetsuisse Faculty, Universität Bern, Länggass-Strasse 122, Postfach, CH-3001 Bern, Switzerland.
Janis
Carole
C
Vilei
Edy M
EM
Bertoni
Giuseppe
G
Frey
Joachim
J
D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D004847:Epithelial Cells / Q000382:microbiology* / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005990:Glycerol / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006861:Hydrogen Peroxide; D008969:Molecular Sequence Data; D009176:Mycoplasma mycoides / Q000145:classification / Q000378:metabolism* / Q000472:pathogenicity; D018384:Oxidative Stress; D014774:Virulence
D001426:Bacterial Proteins; D006861:Hydrogen Peroxide; D005993:Glycerolphosphate Dehydrogenase; C036140:L-alpha-glycerol-phosphate oxidase; D005990:Glycerol