Spinal stenosis: assessment of motor function, VEGF expression and angiogenesis in an experimental model in the rat.
Reduction of blood flow in compressed nerve roots is considered as one important mechanism of induction of neurogenic intermittent claudication in lumbar spinal canal stenosis. Vascular endothelial growth factor (VEGF) is a potent stimulator of angiogenesis, and is increased in expression in hypoxic conditions. The objective of this study was to examine if cauda equina compression affects motor function and induces expression of VEGF and angiogenesis. The cauda equina was compressed by placing a piece of silicone rubber into the L5 epidural space. Walking duration was examined by rota-rod testing. The compressed parts of the cauda equina and L5 dorsal root ganglion (DRG) were removed at 3, 7, 14, or 28 days after surgery, and processed for immunohistochemistry for VEGF and Factor VIII (marker for vascular endothelial cells). Numbers of VEGF-immunoreactive (IR) cells and vascular density were examined. Walking duration was decreased after induction of cauda equina compression. The number of VEGF-IR cells in the cauda equina and DRG was significantly increased at 3, 14, and 28 days after cauda equina compression, compared with sham-operated rats (P < 0.05). Vascular density in the cauda equina was not increased at any of the time points examined. Cauda equina compression decreased walking duration, and induced VEGF expression in nerve roots and DRG.
Watanabe
Kazuyuki
K
Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima City, 960-1295, Japan. kazu-w@fmu.ac.jp
Konno
Shin-Ichi
S
Sekiguchi
Miho
M
Kikuchi
Shin-Ichi
S
D000818:Animals; D002420:Cauda Equina / Q000473:pathology; D002452:Cell Count; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000473:pathology; D005169:Factor VIII / Q000276:immunology / Q000378:metabolism; D005727:Ganglia, Spinal / Q000473:pathology; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D009389:Neovascularization, Pathologic; D051381:Rats; D017207:Rats, Sprague-Dawley; D013130:Spinal Stenosis / Q000503:physiopathology*; D013131:Spine / Q000098:blood supply*; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000276:immunology / Q000378:metabolism*; D016138:Walking
D042461:Vascular Endothelial Growth Factor A; D005169:Factor VIII