title: Hypoxia regulation of facilitated glucose transporter-1 and glucose transporter-3 in mouse chondrocytes mediated by HIF-1alpha.
abstract: OBJECTIVE
  Chondrocytes reside in a hypoxic environment; they utilize glucose as the main energy source. Facilitated glucose transport represents the first rate-limiting step in glucose metabolism.
  
  
  RESULTS
  Here we demonstrated that hypoxia induced an elevated expression level of GLUT-1 and -3 in chondrocytes. Also elevated was hypoxia inducible factor-1alpha (HIF-1alpha) protein level. On the contrary, deletion of HIF-1alpha led to complete loss of the hypoxia-induced gene expression of GLUT-1 and 3.
  
  
  CONCLUSIONS
  These results suggested that chondrocytes had the capacity to detect and respond to low oxygen availability with changes in expression of oxygen-regulated genes, and that hypoxia regulation of facilitated GLUT-1 and -3 was mediated by HIF-1alpha.
authors.affiliation: Shanghai Institute of Traumatology & Orthopaedics and Department of Orthopaedics, Ruijin Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200025, China.
authors.forename: Bu-Fang
authors.initials: BF
authors.lastname: Ren
authors.forename: Lian-Fu
authors.initials: LF
authors.lastname: Deng
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D053674:Aggrecans / Q000378:metabolism; D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003035:Cobalt / Q000494:pharmacology; D024043:Collagen Type II / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D051274:Glucose Transporter Type 3 / Q000378:metabolism*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D053674:Aggrecans; D024043:Collagen Type II; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; C497443:Hif1a protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; C495678:Slc2a1 protein, mouse; C495689:Slc2a3 protein, mouse; D003035:Cobalt; C018021:cobaltous chloride; D005947:Glucose; D010100:Oxygen