[Matrix metalloproteinases inhibitor doxycycline inhibits TGF-beta 1-induced RPE cell migration].
OBJECTIVE
To investigate the effects of matrix metalloproteinases (MMP) inhibitor (Doxycycline) on retinal pigment epithelial (RPE) cell migration induced by transforming growth factor beta 1 (TGF-beta 1).


METHODS
The third to sixth passage human RPE cells cultures were treated with TGF-beta 1 at different concentrations (0.01, 0.10, 1.00, 10.00 microg/L), and the conditioned media were collected after 36 h of TGF-beta 1 exposure. Gelatinase activities in conditioned media were analyzed by zymography. Migration assay was performed in a Boyden chamber with addition of different concentrations of Doxycycline. The number of migrated cells was counted under light microscopy.


RESULTS
RPE cells migration were stimulated by TGF-beta 1 significantly, the number of migrated RPE cells increased about 27% compared with the control (P < 0.01). In the presence of Doxycycline, the RPE cells migration induced by TGF-beta 1 was inhibited and the number of migrated cells was reduced to 50% - 70% compared with control (P < 0.01). TGF-beta 1 also stimulated the secretion of MMP-2 in dose-dependent manner.


CONCLUSIONS
This study indicates that Doxycycline can inhibit migration of RPE cells stimulated by TGF-beta 1. RPE migration induced by TGF-beta 1 may act partially through the stimulation of MMP production.
Zeng
Ai-ping
AP
Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. aiping_zeng@163.com
Zeng
Shui-qing
SQ
Li
Peng-cheng
PC
Cheng
Yang
Y
D002455:Cell Division; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D004318:Doxycycline / Q000494:pharmacology*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000187:drug effects*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
D053773:Transforming Growth Factor beta1; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D004318:Doxycycline