Detection and quantification of soy allergens in food: study of two commercial enzyme-linked immunosorbent assays.
The objective of this study was to evaluate the efficacy of 2 commercially available soy enzyme-linked immunosorbent assays (ELISA) and use them in detecting soy proteins in selected food commodities. Both ELISA kits exhibited high sensitivity. The determined limits of detection (LOD) (approximately 2 and <1 microg/mL for Tepnel Biosystems and Elisa Systems kits, respectively) were lower than those claimed by the manufacturer. Quantification range for both kits was, however, narrower and in a lower concentration range than defined by the kit providers. Our examination revealed a positive cross-reactivity with chickpea proteins and matrix interferences for both kits. The immunoreactivity of soy proteins, when tested by the Tepnel Biosystems kit, was partially reduced by papain and bromelain hydrolysis; it was significantly decreased by protein glycation (>47%). Nondenatured and nonheated soy protein isolate (SPI) samples were also significantly less antigenic than the treated ones.
L'Hocine
Lamia
L
Food Research and Development Centre, Agriculture and Agri-Food Canada, Casavant Blvd. West, St Hyacinthe, QC, J2S 8E3, Canada.
Boye
Joyce Irene
JI
Munyana
Christella
C
D000485:Allergens / Q000032:analysis*; D002138:Calibration / Q000592:standards*; D002623:Chemistry Techniques, Analytical; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards*; D005504:Food Analysis; D005512:Food Hypersensitivity / Q000175:diagnosis*; D006801:Humans; D006868:Hydrolysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D030262:Soybean Proteins / Q000276:immunology*; D013025:Glycine max / Q000737:chemistry / Q000276:immunology
D000485:Allergens; D030262:Soybean Proteins