Transfection of human prostate cancer CA-HPV-10 cells with cytosolic sulfotransferase SULT1E1 affects estrogen signaling and gene transcription.
Human cytosolic sulfotransferase SULT1E1 catalyzes the sulfation of estrogens and estrogenic drugs in human reproductive tissues. Logically, this estrogen-preferring sulfotransferase isoform could play a regulatory role in estrogen signaling activities in human reproductive cells, including the prostate cells. This hypothesis was tested using DNA microarray and real-time reverse transcription-polymerase chain reaction methods in the present work. Potential changes in the transcriptional expression of selected signal transduction-related genes in human prostate cancer CA-HPV-10 cell line after SULT1E1 transfection were examined by DNA microarray methods. Notable changes were observed in the mRNA expression levels of TFRC, a cell membrane transferrin receptor gene, and TMEPAI, a gene encoding a steroid-dependent mRNA product. Expression of TFRC was down-regulated, whereas expression of TMEPAI was up-regulated by SULT1E1 transfection in CA-HPV-10 cells. Data from the current studies also showed that the estrogen-induced estrogen response element activation in CA-HPV-10 cells was repressed after the cells were transfected with SULT1E1. These results indicate that SULT1E1 may function as a transcriptional mediator in human prostate cancer CA-HPV-10 cells.
Kapoor
Ruchita
R
Department of Pharmaceutical Sciences, College of Pharmacy, North Dakota State University, Fargo, ND 58105, USA.
Sheng
Jonathan J
JJ
D015703:Antigens, CD / Q000235:genetics*; D045744:Cell Line, Tumor; D003600:Cytosol; D015536:Down-Regulation; D004958:Estradiol / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000235:genetics; D004967:Estrogens / Q000378:metabolism*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D011471:Prostatic Neoplasms; D012333:RNA, Messenger / Q000378:metabolism; D011990:Receptors, Transferrin / Q000235:genetics*; D020218:Response Elements; D020845:Selective Estrogen Receptor Modulators / Q000494:pharmacology; D015238:Sulfotransferases / Q000235:genetics*; D013629:Tamoxifen / Q000494:pharmacology; D014158:Transcription, Genetic; D014162:Transfection; D015854:Up-Regulation
D015703:Antigens, CD; C068126:CD71 antigen; D047628:Estrogen Receptor alpha; D004967:Estrogens; D008565:Membrane Proteins; C412868:PMEPA1 protein, human; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D020845:Selective Estrogen Receptor Modulators; D013629:Tamoxifen; D004958:Estradiol; D015238:Sulfotransferases; C020122:estrone sulfotransferase