Characterization of a novel Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene.
SH3 domain binding protein 5 like (xSH3BP5L) gene encodes a protein that is a new found member of SH3 domain binding protein family which has been implicated at multiple levels of biological functions. Here, we have characterized Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene in the development of Xenopus laevis. Transcripts of xSH3BP5L were detected at all stages of development and in numerous adult tissues. Whole-mount in situ hybridization demonstrated that xSH3BP5L is expressed at the animal pole from stage-2 onward. Interestingly, translational inhibition of xSH3BP5L using antisense morpholino oligonucleotides (MOs) and overexpression of xSH3BP5L in Xenopus embryos resulted in failed or delayed blastopore closure. Taken together, these data suggested that xSH3BP5L is required for normal embryogenesis of blastopore closure in X. laevis.
Hu
Zhong-Guo
ZG
School of Life Science, and Core Facility of Gene Engineered Mouse, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Sichuan 610041, PR China.
Chen
Ye
Y
Zhou
Qin
Q
Lv
Xiao-Yan
XY
Zhang
Zheng
Z
Wang
Yi-Dong
YD
Xiao
Yan
Y
Guo
Hong
H
Liu
Yu-Hang
YH
Tan
Rui-Zhi
RZ
Li
Qin-Wei
QW
Bian
Guo-Hui
GH
Wei
Yu-Quan
YQ
D000375:Aging / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D008840:Microfilament Proteins / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D014018:Tissue Distribution; D014982:Xenopus laevis / Q000502:physiology*
D008840:Microfilament Proteins; C119201:ponsin