NC2 mobilizes TBP on core promoter TATA boxes.
The general transcription factors (GTFs) of eukaryotic RNA polymerase II, in a process facilitated by regulatory and accessory factors, target promoters through synergistic interactions with core elements. The specific binding of the TATA box-binding protein (TBP) to the TATA box has led to the assumption that GTFs recognize promoters directly, producing a preinitiation complex at a defined position. Using biochemical analysis as well as biophysical single-pair Förster resonance energy transfer, we now provide evidence that negative cofactor-2 (NC2) induces dynamic conformational changes in the TBP-DNA complex that allow it to escape and return to TATA-binding mode. This can lead to movement of TBP along the DNA away from TATA.
Schluesche
Peter
P
Department of Chemistry and Biochemistry and Center for Nanoscience (CeNS), Ludwig Maximilian University, Butenandtstr. 11, 81377 Munich, Germany.
Stelzer
Gertraud
G
Piaia
Elisa
E
Lamb
Don C
DC
Meisterernst
Michael
M
D004247:DNA / Q000737:chemistry; D024202:Electrophoretic Mobility Shift Assay; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D015394:Molecular Structure; D010750:Phosphoproteins / Q000737:chemistry / Q000502:physiology*; D011401:Promoter Regions, Genetic; D016385:TATA Box; D035181:TATA-Box Binding Protein / Q000737:chemistry / Q000502:physiology*; D014157:Transcription Factors / Q000737:chemistry / Q000502:physiology*
D010750:Phosphoproteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors; C075995:down-regulator of transcription 1; D004247:DNA