title: [Effect of human oviductal embryotrophic factors on gene expression of mouse preimplantation embryos].
abstract: OBJECTIVE
  To investigate the effect of embryotrophic factors (ETF) from human oviductal cells on gene expression of mouse early developmental embryos and discuss the role of fallopian tube in early development of embryos.
  
  
  METHODS
  ETF was isolated from conditioned medium of human oviductal cell line by sequential liquid chromatographic systems. Mouse embryos were treated by ETF in vitro. Using differential display RT-PCR, the gene expression of embryos treated by ETF was compared with embryos without ETF treatment. The differentially expressed genes were separated, re-amplified, cloned and sequenced.
  
  
  RESULTS
  Gene expression profiles of embryos with ETF treatment was different from embryos without this treatment. Eight differentially expressed genes were cloned and sequenced. These genes functioned in RNA degradation, synthesis, splicing, protein trafficking, cellular differentiation and embryo development.
  
  
  CONCLUSIONS
  Embryotrophic factors from human oviductal cells affect gene expression of early developmental embryos. The human oviductal cells play wide roles in early developmental stages of embryos.
authors.affiliation: Department of Obstetrics and Gynecology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. yqyao@126.com
authors.forename: Yuan-Qing
authors.initials: YQ
authors.lastname: Yao
authors.forename: Kai-Fai
authors.initials: KF
authors.lastname: Lee
authors.forename: Jia-Seng
authors.initials: JS
authors.lastname: Xu
authors.forename: Pak-Chung
authors.initials: PC
authors.lastname: Ho
authors.forename: Shu-Biu
authors.initials: SB
authors.lastname: Yeung
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics; D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000187:drug effects* / Q000235:genetics; D005187:Fallopian Tubes / Q000166:cytology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006023:Glycoproteins / Q000235:genetics; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017077:Culture Media, Conditioned; D006023:Glycoproteins; D006133:Growth Substances; D019883:Alkyl and Aryl Transferases; C075607:Rab geranylgeranyltransferase