The Hypernodulating nts mutation induces jasmonate synthetic pathway in soybean leaves.
Symbiotic nitrogen fixation with nitrogen-fixing bacteria in the root nodules is a distinctly beneficial metabolic process in legume plants. Legumes control the nodule number and nodulation zone through a systemic negative regulatory system between shoot and root. Mutation in the soybean NTS gene encoding GmNARK, a CLAVATA1-like serine/threonine receptor-like kinase, causes excessive nodule development called hypernodulation. To examine the effect of nts mutation on the gene expression profile in the leaves, suppression subtractive hybridization was performed with the trifoliate leaves of nts mutant 'SS2-2' and the wild-type (WT) parent Sinpaldalkong2, and 75 EST clones that were highly expressed in the leaves of the SS2-2 mutant were identified. Interestingly, the expression of jasmonate (JA)-responsive genes such as vspA, vspB, and Lox2 were upregulated, whereas that of a salicylate-responsive gene PR1a was suppressed in the SS2-2 mutant. In addition, the level of JA was about two-fold higher in the leaves of the SS2-2 mutant than in those of the WT under natural growth conditions. Moreover, the JA-responsive gene expression persists in the leaves of SS2-2 mutant without rhizobia infection in the roots. Taken together, our results suggest that the nts mutation increases JA synthesis in mature leaves and consequently leads to constitutive expression of JA-responsive genes which is irrelevant to hypernodulation in the root.
Seo
Hak Soo
HS
Department of Plant Science and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea.
Li
Jinjie
J
Lee
Sun-Young
SY
Yu
Jae-Woong
JW
Kim
Kil-Hyun
KH
Lee
Suk-Ha
SH
Lee
In-Jung
IJ
Paek
Nam-Chon
NC
D000085:Acetates / Q000494:pharmacology; D002999:Clone Cells; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D015723:Gene Library; D017343:Genes, Plant; D009154:Mutation / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D054883:Oxylipins / Q000378:metabolism* / Q000494:pharmacology; D010641:Phenotype; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D053204:Root Nodules, Plant / Q000187:drug effects / Q000378:metabolism*; D013025:Glycine max / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid; C072239:methyl jasmonate