[Research on isolation and purification of total iridoid glycosides from herbal Lamiophlomis rotata].
OBJECTIVE
Enriching the hemostatic active part total iridoid glycosides from the aqueous extract of herbal Lamiophlomis rotata.


METHODS
First derivative spectrophotometry was used as the detection method and the content of total iridoid glycosides was used as tracking target. The abilities of absorption and anti-absorption of ten different types of macroreticular resin were compared, after being used to absorb the iridoid glycosides from aqueous extract of herbal L. rotata. The best purification process was optimized.


RESULTS
With the optimized purification process, the content of total iridoid glycosides was 67.53%.


CONCLUSIONS
XDA-1 macroreticular resin has good ability of absorption and anti-absorption for iridoid glycosides and 50% ethanol was the best solvent for anti-absorption. The macroreticular resin could be reused.
Li
Mao-xing
MX
Department of Pharmacy, Lanzhou General Hospital of PLA, Lanzhou 730050, China. lm730530xing@yahoo.com.cn
Jia
Zheng-ping
ZP
Hu
Zhi-de
ZD
Zhang
Ru-xue
RX
Ren
Xiao-ling
XL
Wang
Juan
J
Hu
Jing
J
D006027:Glycosides / Q000302:isolation & purification*; D039823:Iridoids / Q000302:isolation & purification*; D019686:Lamiaceae / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012117:Resins, Synthetic; D013678:Technology, Pharmaceutical / Q000379:methods
D006027:Glycosides; D039823:Iridoids; D012117:Resins, Synthetic