title: [Correlation between load of polyomavirus and hemorrhagic cystitis].
abstract: OBJECTIVE
  To study the correlation between polyoma virus load and hemorrhagic cystitis after allogeneic stem cells transplantation for prevention of hemorrhagic cystitis.
  
  
  METHODS
  Blood and urine specimens were collected from 40 healthy persons, 40 patient with stem cells transplantation and 20 cases complicated with hemorrhagic cystitis for determination of VP1 gene of polyomaviruses BK virus (BKV)/Jamestown Canyon virus (JCV) and simian virus 40 (SV40) by polymerase chain reaction (PCR) and EvaGreen stain fluorescence quantitative assay.
  
  
  RESULTS
  In the peripheral blood, all genes of BKV/JCV and SV40 were negative, while BKV gene in urine and blood from healthy persons and patient with stem cells transplantation was 15% (6/40) and 100% (40/40), respectively. The gene of JCV was positive in 10% (4/40) and 12% (5/40), the gene of SV40 was negative.
  
  
  CONCLUSIONS
  Genes of BKV and JCV was detectable in urine specimens of healthy persons and there was a correlation between the load of polyomavirus and incidence of hemorrhagic cystitis.
authors.affiliation: Beijing Dao-Pei Hospital, Beijing, China. cr.21@163.com
authors.forename: Chun-Rong
authors.initials: CR
authors.lastname: Tong
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Teng
authors.forename: Hong-Xing
authors.initials: HX
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Cai
authors.forename: Si-Kun
authors.initials: SK
authors.lastname: Ma
authors.forename: Cheng-Liang
authors.initials: CL
authors.lastname: Zhen
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
authors.forename: Dao-Pei
authors.initials: DP
authors.lastname: Lu
mesh_terms: D036022:Capsid Proteins / Q000235:genetics; D003556:Cystitis / Q000175:diagnosis* / Q000209:etiology / Q000821:virology; D004279:DNA, Viral / Q000097:blood / Q000235:genetics / Q000652:urine; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D006470:Hemorrhage / Q000175:diagnosis* / Q000209:etiology / Q000821:virology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011120:Polyomavirus / Q000235:genetics / Q000254:growth & development*; D027601:Polyomavirus Infections / Q000150:complications / Q000821:virology*; D019562:Viral Load
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; C062481:VP1 protein, polyomavirus