title: Screening of the osmotic pressure-inducible promoter regions from the whole genome of Escherichia coli by using a novel cloning method.
abstract: Promoters belong to the most important regulatory domains in genomic sequences. A novel cloning method is described to produce various DNA fragments with the average size about 100 bp, which differ by 1 bp, for screening of the osmotic pressure-inducible promoters from the genome of Escherichia coli. An osmotic pressure-inducible promoter sequence was found. A mutation in this promoter sequence, introduced by site-specific mutagenesis, abolished its activity under the high osmotic pressure conditions. The method can thus be used for finding particular promoter sequences in the E. coli genome.
authors.affiliation: CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, P.R. China. zjwang@shmu.edu.cn
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Jin
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Wegrzyn
authors.forename: Alicja
authors.initials: A
authors.lastname: Wegrzyn
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D016680:Genome, Bacterial; D008957:Models, Genetic; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009997:Osmotic Pressure; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial