Early detection of pregnancy and embryonic loss in dairy cattle by ELISA tests.
Over a 25-month period 8118 blood samples were assayed for the presence of the serum pregnancy specific-protein B (PSPB) and progesterone (P4) concentrations on three Hungarian large-scale dairy farms. Pregnancy (n = 4085) was checked by BioPRYN assay at 30-36 days post-insemination (PI). Samples from all cows that tested not pregnant and from cows with an optical density (OD) reading in the BioPRYN test that was between 0% and 30% above the cutoff OD value were tested for serum P4 concentration. According to serum P4 concentration, cows were assigned to three categories: high (>4 ng/ml), medium (2-4 ng/ml) and low (<2 ng/ml) serum progesterone. The authors predicted a presumed (low) or possible (medium) late embryonic loss (LEL) or maintenance of the pregnancy (high). A total of 710 LELs were detected (17.4%) and 31.8% of them were predicted because of a low OD value at 30-36 days after insemination. Lower PSPB serum level significantly refers for LEL (p < 0.0001). The prediction rate for the true embryonic loss was 31.8% when OD cutoff from 0% to + 30% of cutoff was examined while it was 62.5% when the threshold was OD cutoff of 0% to 10% of cutoff. The authors conclude that BioPRYN was useful for prediction of a part of LEL in dairy cows and serum P4 concentration in these cows related to the rate of LEL.
Gábor
G
G
Research Institute for Animal Breeding and Nutrition, Gesztenyes Str. 1, Herceghalom, Hungary. h12617gab@helka.iif.hu
Tóth
F
F
Ozsvári
L
L
Abonyi-Tóth
Zs
Z
Sasser
R G
RG
D000034:Abortion, Veterinary / Q000097:blood*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002417:Cattle / Q000097:blood / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D007315:Insemination, Artificial / Q000662:veterinary; D011237:Predictive Value of Tests; D011247:Pregnancy; D011258:Pregnancy Tests / Q000379:methods / Q000592:standards / Q000662:veterinary*; D011270:Pregnancy, Animal / Q000097:blood*; D011268:Pregnancy-Specific beta 1-Glycoproteins; D011374:Progesterone / Q000097:blood*
D015415:Biomarkers; D011268:Pregnancy-Specific beta 1-Glycoproteins; D011374:Progesterone