Analysis of metformin dosage formulations by capillary electrophoresis at nano scale detection.
An inexpensive, rapid and reproducible capillary electrophoretic method has been developed and validated for the determination of metformin in pharmaceutical preparations. The method was developed utilizing a fused silica capillary (60 cm x 50 microm I.D.), phosphate buffer (50 mM, 3.0 pH)-acetonitrile (95:5, v/v) as background electrolyte (BGE), 20 kV applied voltage with UV detection at 254 nm and at a working temperature of 23 +/- 1 degrees C. Linearity was observed in the concentration range from 100 ng/L to 5 microg/L, with a correlation coefficient (R2) of 0.9998. The limits of detection and quantification achieved were 60 and 100 ng/mL, respectively. The recovery of metformin from pharmaceutical preparations was 99.1%. These validation parameters demonstrate the precision of the method and its suitability for the determination of metformin in pharmaceutical tablet formulations.
Ali
Imran
I
Department of Chemistry, Jamia Millia Islamia (A Central University), Jamia Nagar, New Delhi, India. drimran_ali@yahoo.com
Aboul-Enein
Hassan Y
HY
Gupta
Vinod K
VK
D019075:Electrophoresis, Capillary / Q000191:economics / Q000295:instrumentation / Q000379:methods; D016014:Linear Models; D008687:Metformin / Q000032:analysis*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions / Q000737:chemistry; D013607:Tablets
D004364:Pharmaceutical Preparations; D012996:Solutions; D013607:Tablets; D008687:Metformin