title: Role of N-terminal domain and accessory subunits in controlling deactivation-inactivation coupling of Kv4.2 channels.
abstract: We examined the relationship between deactivation and inactivation in Kv4.2 channels. In particular, we were interested in the role of a Kv4.2 N-terminal domain and accessory subunits in controlling macroscopic gating kinetics and asked if the effects of N-terminal deletion and accessory subunit coexpression conform to a kinetic coupling of deactivation and inactivation. We expressed Kv4.2 wild-type channels and N-terminal deletion mutants in the absence and presence of Kv channel interacting proteins (KChIPs) and dipeptidyl aminopeptidase-like proteins (DPPs) in human embryonic kidney 293 cells. Kv4.2-mediated A-type currents at positive and deactivation tail currents at negative membrane potentials were recorded under whole-cell voltage-clamp and analyzed by multi-exponential fitting. The observed changes in Kv4.2 macroscopic inactivation kinetics caused by N-terminal deletion, accessory subunit coexpression, or a combination of the two maneuvers were compared with respective changes in deactivation kinetics. Extensive correlation analyses indicated that modulatory effects on deactivation closely parallel respective effects on inactivation, including both onset and recovery kinetics. Searching for the structural determinants, which control deactivation and inactivation, we found that in a Kv4.2 Delta 2-10 N-terminal deletion mutant both the initial rapid phase of macroscopic inactivation and tail current deactivation were slowed. On the other hand, the intermediate and slow phase of A-type current decay, recovery from inactivation, and tail current decay kinetics were accelerated in Kv4.2 Delta 2-10 by KChIP2 and DPPX. Thus, a Kv4.2 N-terminal domain, which may control both inactivation and deactivation, is not necessary for active modulation of current kinetics by accessory subunits. Our results further suggest distinct mechanisms for Kv4.2 gating modulation by KChIPs and DPPs.
authors.affiliation: Zentrum für Molekulare Neurobiologie, Institut für Neurale Signalverarbeitung, Universitätsklinikum Hamburg-Eppendorf, 20246 Hamburg, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Barghaan
authors.forename: Magdalini
authors.initials: M
authors.lastname: Tozakidou
authors.forename: Heimo
authors.initials: H
authors.lastname: Ehmke
authors.forename: Robert
authors.initials: R
authors.lastname: Bähring
mesh_terms: D002460:Cell Line; D003198:Computer Simulation; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology*; D007668:Kidney / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D008954:Models, Biological; D008956:Models, Chemical; D011487:Protein Conformation; D021122:Protein Subunits; D051672:Shal Potassium Channels / Q000737:chemistry* / Q000502:physiology* / Q000648:ultrastructure; D013329:Structure-Activity Relationship
chemical_list: D021122:Protein Subunits; D051672:Shal Potassium Channels