Controlling the charge and organization of anionic lipid bilayers: effect of monovalent and divalent ions.
It is shown that the organization of lipid bilayers containing phosphatidic acid (PA) and phosphatidlycholine (PC) can be controlled by altering the monovalent and divalent ion concentrations. At high pH and/or calcium concentration, 1:1 Ca(2+)-PA(2-) complexes form; these complexes demix, and PA-rich and PC-rich regions are observable with epifluorescence microscopy. The results are compared with predictions from electrostatic theory. It is noted that the complex formation correlates in a roughly linear fashion with the monovalent/divalent ion ratio, a parameter that cells adjust.
Lamberson
Emily R
ER
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Cambrea
Lee R
LR
Hovis
Jennifer S
JS
D000838:Anions; D002118:Calcium / Q000737:chemistry; D002412:Cations; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers; D008856:Microscopy, Fluorescence; D010712:Phosphatidic Acids / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry
D000838:Anions; D002412:Cations; D008051:Lipid Bilayers; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D002118:Calcium