title: Platelet factor-4 is an indicator of blood count recovery in acute myeloid leukemia patients in complete remission.
abstract: To investigate whether serum biomarkers can be used to indicate the responsiveness of acute myeloid leukemia to remission induction chemotherapy, we performed MALDI-TOF protein profile analysis of patient sera. The resulting spectra revealed a protein (or peptide) peak at m/z 7764 that varied in intensity; its intensity was much higher in samples from patients in complete remission than in those from patients with resistant disease or in samples taken prior to treatment (at the time of diagnosis). Using fractionation, trypsin digestion, MS/MS, and protein molecular weight analyses, we identified the m/z 7764 protein as platelet factor-4 (PF4). This identification was confirmed by a magnetic bead-based MALDI immunoassay. Statistical comparison of PF4 levels and platelet counts in patient sera revealed a significant positive correlation between the two variables. This study demonstrates that PF4 protein levels are a good indicator for the recovery of blood count in the complete remission of acute myeloid leukemia. The linear positive correlation curve indicates that blood count recovery of platelets to >100,000/mm(3) is equivalent to a serum PF4 recovery level of >2.492 microg/ml.
authors.affiliation: Proteome Analysis Team, Korea Basic Science Institute, Daejeon 305-333, Korea.
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Ho-Jun
authors.initials: HJ
authors.lastname: Song
authors.forename: Hoi-Jeong
authors.initials: HJ
authors.lastname: Lim
authors.forename: Myung-Geun
authors.initials: MG
authors.lastname: Shin
authors.forename: Jae Seong
authors.initials: JS
authors.lastname: Kim
authors.forename: Hyeoung-Joon
authors.initials: HJ
authors.lastname: Kim
authors.forename: Baik Yoon
authors.initials: BY
authors.lastname: Kim
authors.forename: Seung-won
authors.initials: SW
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D001772:Blood Cell Count; D005591:Chemical Fractionation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007118:Immunoassay; D015470:Leukemia, Myeloid, Acute / Q000097:blood* / Q000188:drug therapy*; D008280:Magnetics; D008297:Male; D008863:Microspheres; D008875:Middle Aged; D008969:Molecular Sequence Data; D010976:Platelet Count; D010978:Platelet Factor 4 / Q000097:blood* / Q000737:chemistry; D012044:Regression Analysis; D012074:Remission Induction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010978:Platelet Factor 4