Molecular cytogenetics of Salminus fish (Characiformes) based on 5S and 18S rRNA genes hybridization, fluorochrome staining and C-banding.
Karyotype, mapping of nucleolar and 5S rRNA genes and distribution of constitutive heterochromatin supposedly AT-rich were characterized on two isolate populations of Salminus brasiliensis, the biggest characid fish, and three population of Salminus hilarii. The diploid number 2n=50 and the karyotype formulae (10M+20SM+20ST/A) were the same to Salminus species studied. The position of 18S rDNA cluster identified by FISH coincide with chromomycin A3 labeling (CMA+) in the long arm telomeric portion of sixth pair. Subtle differences for the disposal of the 5S rRNA gene in the chromosome of the Salminus are presented. The distribution of the constitutive heterochromatins and DA/DAPI+ bands are described.
Souza
Issakar Lima
IL
Departamento de Ciências Biológicas e da Saúde, Instituto Universitário do Araguaia, Universidade Federal de Mato Grosso, Rodovia MT 100, km 3.5, CEP 78698-000, Pontal do Araguaia MT, Brazil. issakar@pq.cnpq.br
Santos-Silva
Ludier Kesser
LK
Venere
Paulo César
PC
Moreira-Filho
Orlando
O
D000818:Animals; D002871:Chromosome Banding; D002875:Chromosomes / Q000235:genetics; D020732:Cytogenetic Analysis; D005399:Fishes / Q000145:classification / Q000235:genetics*; D005456:Fluorescent Dyes / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D009693:Nucleic Acid Hybridization; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D013194:Staining and Labeling
D005456:Fluorescent Dyes; D012337:RNA, Ribosomal, 18S; D012341:RNA, Ribosomal, 5S