title: Imaging Drosophila gene activation and polymerase pausing in vivo.
abstract: Since the early 1960s, imaging studies of Drosophila sp. polytene chromosomes have provided unique views of gene transcription in vivo. The dramatic changes in chromatin structure that accompany gene activation can be visualized as chromosome puffs. Now, live-cell imaging techniques coupled with protein-DNA crosslinking assays on a genome-wide scale allow more detailed mechanistic questions to be addressed and are prompting the re-evaluation of models of transcription regulation in both Drosophila and mammals.
authors.affiliation: Molecular Biology and Genetics, 416 Biotechnology Building, Cornell University, Ithaca, New York 14853, USA. jtl10@cornell.edu
authors.forename: John T
authors.initials: JT
authors.lastname: Lis
mesh_terms: D000818:Animals; D004330:Drosophila / Q000166:cytology / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D029721:Drosophila Proteins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012319:RNA Polymerase II / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics; D015533:Transcriptional Activation
chemical_list: D029721:Drosophila Proteins; D012319:RNA Polymerase II