title: Sequence-specific Methyltransferase-Induced Labelling (SMILing) of plasmid DNA for studying cell transfection.
abstract: Plasmid DNA (pUC19 and pBR322) was sequence-specifically, covalently labelled with Cy3 fluorophores using a newly synthesised N-adenosylaziridine cofactor and the DNA methyltransferase M.TaqI. The fluorescently labelled plasmids were used for transfection of mammalian cells and their intracellular distribution was visualised by epifluorescence and confocal fluorescence microscopy. Although these prokaryotic plasmids do not contain nuclear import sequences, translocation into the nuclei was observed.
authors.affiliation: Laboratoire de Chimie des Interactions Moléculaires, Collège de France, Paris, France.
authors.forename: Falk H-G
authors.initials: FH
authors.lastname: Schmidt
authors.forename: Michael
authors.initials: M
authors.lastname: Hüben
authors.forename: Basar
authors.initials: B
authors.lastname: Gider
authors.forename: François
authors.initials: F
authors.lastname: Renault
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Teulade-Fichou
authors.forename: Elmar
authors.initials: E
authors.lastname: Weinhold
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D001483:Base Sequence; D002232:Carbocyanines; D002478:Cells, Cultured; D015254:DNA Modification Methylases; D006801:Humans; D015336:Molecular Probe Techniques; D010957:Plasmids; D019914:Taq Polymerase; D014162:Transfection
chemical_list: D002232:Carbocyanines; C089187:cyanine dye 3; D015254:DNA Modification Methylases; D019914:Taq Polymerase