Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source.
We developed a novel scheme for two-photon fluorescence bioimaging. We generated supercontinuum (SC) light at wavelengths of 600 to 1200 nm with 774-nm light pulses from a compact turn-key semiconductor laser picosecond light pulse source that we developed. The supercontinuum light was sliced at around 1030- and 920-nm wavelengths and was amplified to kW-peak-power level using laboratory-made low-nonlinear-effects optical fiber amplifiers. We successfully demonstrated two-photon fluorescence bioimaging of mouse brain neurons containing green fluorescent protein (GFP).
Yokoyama
Hiroyuki
H
Tohoku University, New Industry Creation Hatchery Center (NICHe), 6-6-10 Aramaki-Aoba, Aoba-ku, Sendai 980-8579, Japan. yoko@niche.tohoku.ac.jp
Tsubokawa
Hiroshi
H
Guo
Hengchang
H
Shikata
Jun-ichi
J
Sato
Ki-ichi
K
Takashima
Keijiro
K
Kashiwagi
Kaori
K
Saito
Naoaki
N
Taniguchi
Hirokazu
H
Ito
Hiromasa
H
D000818:Animals; D001921:Brain / Q000166:cytology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007834:Lasers; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D009474:Neurons / Q000166:cytology*; D012666:Semiconductors