Separation of nucleic acid precursors on an amphoteric surfactant modified monolith using combined eluent flow, pH and concentration gradient.
An RP monolithic column coated with an amphoteric carboxybetaine type surfactant has been used with a combined triple eluent concentration, pH and flow gradient ion chromatography technique for the simultaneous separation of up to 18 nucleotides, nucleosides and nucleobases. The separation of up to eight precursors on a 1 cm long monolithic microcolumn using the combined gradient approach is also shown. The method was applied to the separation of the above nucleic acid precursors in perchloric acid extracts of yeastolates samples.
Nesterenko
Ekaterina P
EP
National Centre for Sensor Research, School of Chemical Sciences, Dublin City University, Glasnevin, Dublin, Ireland.
Guivarc'h
Céline
C
Duffy
Cepta
C
Paull
Brett
B
D001622:Betaine / Q000031:analogs & derivatives / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D006863:Hydrogen-Ion Concentration; D009694:Nucleic Acid Precursors / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*; D013997:Time Factors; D015003:Yeasts / Q000737:chemistry
D009694:Nucleic Acid Precursors; D009711:Nucleotides; D013501:Surface-Active Agents; D001622:Betaine; D002118:Calcium