Elevated mature macrophage expression of human ABHD2 gene in vulnerable plaque.
We previously reported that the mouse alpha/beta hydrolase domain containing 2 (Abhd2) was expressed in smooth muscle cells (SMCs) which suppressed their migration and inhibited the development of intimal hyperplasia by cuff placement; however, the role of ABHD2 in human remains to be elucidated. In this study, we examined ABHD2 expression in the human coronary atherosclerotic lesions of the patients with unstable angina (UA) and stable angina (SA). Our results showed that the ABHD2 was expressed in atherosclerotic lesions, and that the ABHD2 expression was significantly higher in the patients with UA than with SA. Immunohistochemistry analysis revealed abundant expression of ABHD2 in macrophages, but low expression in SMCs of atherosclerotic lesions. Using human vascular primary culture cell lines, we also demonstrated that the expression of ABHD2 was significantly higher in macrophages than in SMCs, and that the expression of ABHD2 significantly increased proportionally with differentiation from monocyte into macrophage.
Miyata
Keishi
K
Department of Developmental Genetics, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.
Nakayama
Masafumi
M
Mizuta
Shoko
S
Hokimoto
Seiji
S
Sugamura
Koichi
K
Oshima
Shuichi
S
Oike
Yuichi
Y
Sugiyama
Seigo
S
Ogawa
Hisao
H
Yamamura
Ken-ichi
K
D002478:Cells, Cultured; D003324:Coronary Artery Disease / Q000378:metabolism*; D003331:Coronary Vessels / Q000378:metabolism*; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D015854:Up-Regulation
D006867:Hydrolases; C525115:ABHD2 protein, human