Identification of the flavoprotein of succinate dehydrogenase and aconitase as in vitro mitochondrial substrates of Fgr tyrosine kinase.
Overlooked until recently, mitochondrial protein phosphorylation is now emerging as a key post-translational mechanism in the regulation of mitochondrial functions. In particular, tyrosine phosphorylation represents a promising field to discover new mechanisms of bioenergetic regulation. Tyrosine kinases belonging to the Src kinase family have been observed in mitochondrial compartments, however their substrates are almost unknown. Here, we provide evidence that the flavoprotein of succinate dehydrogenase and aconitase are "in vitro" substrates of Fgr tyrosine kinase. Fgr phosphorylates flavoprotein of succinate dehydrogenase at Y535 and Y596 and aconitase at Y71, Y544 and Y665. The significance of these findings is discussed.
Salvi
Mauro
M
Department of Biological Chemistry, University of Padova, Viale G Colombo 3, 35121, Padova, Italy. mauro.salvi@unipd.it
Morrice
Nick A
NA
Brunati
Anna Maria
AM
Toninello
Antonio
A
D000154:Aconitate Hydratase / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D005420:Flavoproteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051381:Rats; D013385:Succinate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D019061:src-Family Kinases / Q000378:metabolism*
D005420:Flavoproteins; D011518:Proto-Oncogene Proteins; D013385:Succinate Dehydrogenase; C053102:proto-oncogene proteins c-fgr; D019061:src-Family Kinases; D000154:Aconitate Hydratase