Transient receptor potential vanilloid type 2 (TRPV2) expression in normal urothelium and in urothelial carcinoma of human bladder: correlation with the pathologic stage.
OBJECTIVE
To evaluate the expression of transient receptor potential vanilloid type 2 (TRPV2) in normal human bladder and urothelial carcinoma (UC) tissues.


METHODS
Bladder specimens were obtained by transurethral resection or radical cystectomy. TRPV2 mRNA expression in normal human urothelial cells (NHUCs), UC cell lines, and formalin-fixed paraffin-embedded normal (n=6) and cancer bladder tissues (n=58) was evaluated by polymerase chain reaction (PCR) and quantitative real-time PCR (RT-PCR). TRPV2 protein expression was assessed by cytofluorimetric and confocal microscopy analyses in NHUCs and UC cells and by Western blotting and immunohistochemistry in normal and UC tissues.


RESULTS
Enhanced TRPV2 mRNA and protein expression was found in high-grade and -stage UC specimens and UC cell lines. Both the full-length TRPV2 (hTRPV2) and a short splice-variant (s-TRPV2) were detected in NHUC and normal bladder specimens, whereas a progressive decline of s-TRPV2 in pTa, pT1, and pT2 stages was observed, up to a complete loss in pT3 and pT4 UC specimens.


CONCLUSIONS
Normal human urothelial cells and bladder tissue specimens express TRPV2 at both the mRNA and protein levels. A progressive loss of s-TRPV2 accompanied by a marked increase of hTRPV2 expression was found in high-grade and -stage UC tissues.
Caprodossi
Sara
S
Department of Experimental Medicine and Public Health, University of Camerino, Camerino (MC), Italy.
Lucciarini
Roberta
R
Amantini
Consuelo
C
Nabissi
Massimo
M
Canesin
Giacomo
G
Ballarini
Patrizia
P
Di Spilimbergo
Adriana
A
Cardarelli
Marco Andrea
MA
Servi
Lucilla
L
Mammana
Gabriele
G
Santoni
Giorgio
G
D000704:Analysis of Variance; D014408:Biomarkers, Tumor / Q000032:analysis; D015153:Blotting, Western; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D016009:Chi-Square Distribution; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D009367:Neoplasm Staging; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050916:TRPV Cation Channels / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D019459:Urothelium / Q000378:metabolism* / Q000473:pathology*
D014408:Biomarkers, Tumor; D050916:TRPV Cation Channels; C400769:TRPV2 protein, human