[Establishment of a colloid gold-immunochromatography assay for detection of type Asia I foot-and-mouth disease virus].
OBJECTIVE
To establish a colloid gold-immunochromatography assay (GICA) for detecting type Asia I foot-and-mouth disease virus (FMDV).


METHODS
Colloidal gold was obtained by reducing the gold chloride with sodium citrate and then it was coupled with the purified anti-FMDV type Asia I antibody.The purified anti-FMDV type Asia I antibody and the goat anti-Guinea pig IgG were wrapped onto the nitrocellulose membrane as test line (T line) and control line (C line). The GICA strip was assembled with the purified antibody labelled with colloidal gold and the nitrocellulose containing antibody. The sensitivity, specificity and stability of GICA strip were evaluated in the diagnosis of FMD viral antigen from clinical samples.


RESULTS
The strip was highly sensitive to FMDV type Asia I(0.116 mg/L) and it had the same result for positive specimens tested thrice. Cross tests proved no cross reaction was found with other serotype FMDV and Swine vesicular disease (SVD) antigen.The corresponding rate of GICA with RIHA was 96.87% for detecting the field sample. The stably test found the strip could be stored for 12 months.


CONCLUSIONS
The GICA strip is a simple and rapid immunochromatographic test strip for the detection of FMDV type Asia I with high sensitivity and specificity.
Jiang
Tao
T
Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of Chinese Academy of Agrcultrual Sciences, Lanzhou 730046, China.
Liang
Zhong
Z
Chen
Juan
J
He
Ji-Jun
JJ
Lu
Lü
L
Ma
Wei-Min
WM
Liu
Zai-Xin
ZX
Liu
Xiang-Tao
XT
D000818:Animals; D002845:Chromatography / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005536:Foot-and-Mouth Disease / Q000175:diagnosis; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology / Q000302:isolation & purification*; D017979:Gold Colloid; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis / Q000302:isolation & purification; D011934:Reagent Strips / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D017979:Gold Colloid; D007074:Immunoglobulin G; D011934:Reagent Strips