Impact assessment of bisphenol A on lignin-modifying enzymes by basidiomycete Trametes versicolor.
The impact of different concentrations of bisphenol A (BPA) was evaluated on growth of the white-rot basidiomycete, Trametes versicolor, and on the expression of genes encoding lignin-modifying enzyme (LME) activities. Effective doses (EDs) were obtained from fungal growth rate to monitor LME activities and the expression levels of their encoding genes. The fungus showed mycelial growth at concentrations of up to 300 microg ml(-1) of BPA with an ED50 value of 185 microg ml(-1). The LME activities were stimulated by BPA concentrations up to 300 microg ml(-1). The lignin peroxidase (LIP) encoding gene may be sensitive to BPA stress.
Takamiya
Minako
M
Chemical Management Centre, National Institute of Technology and Evaluation, 2-49-10 Nishihara, Shibuya-ku, Tokyo 151-0066, Japan. takimiya-minako@nite.go.jp
Magan
Naresh
N
Warner
Philip J
PJ
D001559:Benzhydryl Compounds; D004785:Environmental Pollutants / Q000633:toxicity*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D042845:Laccase / Q000235:genetics / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism; D010636:Phenols / Q000633:toxicity*; D020072:Polyporales / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D012313:RNA / Q000378:metabolism; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004785:Environmental Pollutants; D042845:Laccase; D008031:Lignin; D010544:Peroxidases; D010636:Phenols; D012313:RNA; C042858:lignin peroxidase; C051129:manganese peroxidase; C006780:bisphenol A; D000098826:Bisphenol A Compounds