title: Usefulness of antibodies to deamidated gliadin peptides in celiac disease diagnosis and follow-up.
abstract: The prevalence of the recently described deamidated gliadin peptide antibodies was compared with that of the routinely used antigliadin, antiendomysial, and tissue transglutaminase antibodies in the sera of 128 untreated celiac patients and 134 controls. Sensitivity and specificity for celiac disease were 83.6 and 90.3% for IgA and 84.4 and 98.5% for IgG antibodies to deamidated gliadin peptides. The new test displayed higher diagnostic accuracy than antigliadin antibodies and, although less sensitive than antiendomysial and tissue transglutaminase antibodies, showed significantly higher specificity than tissue transglutaminase antibodies (P < 0.001). Persistence of peptide antibodies after gluten withdrawal was an expression of low compliance with the diet and of the lack of improvement of the intestinal mucosa. The combined use of tissue transglutaminase and deamidated gliadin peptide antibodies seems to be a very useful tool for celiac disease diagnosis. Moreover, antibodies to deamidated gliadin peptides can be helpful in disease follow-up.
authors.affiliation: Department of Gastroenterology and Internal Medicine, S. Orsola-Malpighi Hospital, University of Bologna, Via Massarenti, 9, Bologna 40138, Italy. uvolta@aosp.bo.it
authors.forename: Umberto
authors.initials: U
authors.lastname: Volta
authors.forename: Alessandro
authors.initials: A
authors.lastname: Granito
authors.forename: Erica
authors.initials: E
authors.lastname: Fiorini
authors.forename: Claudia
authors.initials: C
authors.lastname: Parisi
authors.forename: Maria
authors.initials: M
authors.lastname: Piscaglia
authors.forename: Georgios
authors.initials: G
authors.lastname: Pappas
authors.forename: Paolo
authors.initials: P
authors.lastname: Muratori
authors.forename: Francesco B
authors.initials: FB
authors.lastname: Bianchi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002446:Celiac Disease / Q000175:diagnosis* / Q000276:immunology; D016009:Chi-Square Distribution; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005903:Gliadin / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D005903:Gliadin