title: Serum prolidase and IGF-1 as non-invasive markers of hepatic fibrosis during four different periods after bile-duct ligation in rats.
abstract: OBJECTIVE
  Our aim was to study the correlation of serum prolidase and insulin like growth factor-1 to liver collagen and assess their utility as markers of fibrosis during four different periods of hepatic injury and fibrosis after bile-duct ligation in rats.
  
  
  METHODS
  Forty-eight Wistar albino rats were included in the study and divided into six groups. Seven rats served as the control group (Control), while seven rats had a sham operation (Sham group). Thirty-four rats underwent bile-duct ligation. Bile-duct ligated (BDL) animals were sacrificed at the end of the first week (Group 1; n = 8), second week (Group 2; n = 8), third week (Group 3; n = 9), or fourth week (Group 4; n = 9) after BDL. Liver collagen, liver prolidase, and serum prolidase and IGF-I, were determined.
  
  
  RESULTS
  There was a positive correlation between liver collagen and serum prolidase (r(s): 0.843, P < 0.001) levels and a negative correlation among liver collagen and serum IGF-1 levels (r(s): -0.667, P < 0.001). The peak levels of liver collagen and serum prolidase were reached in the third week while the lowest levels of IGF-1 were found at the end of the third week.
  
  
  CONCLUSIONS
  Serum prolidase and IGF-1 either independently or in combination correlate with liver collagen content in hepatic fibrosis.
authors.affiliation: Division of Gastroenterology, Gümüşsuyu Military Hospital, Istanbul, Turkey. ortarcin@hotmail.com
authors.forename: Orhan
authors.initials: O
authors.lastname: Tarçin
authors.forename: Nursal
authors.initials: N
authors.lastname: Gedik
authors.forename: Berna
authors.initials: B
authors.lastname: Karakoyun
authors.forename: Veysel
authors.initials: V
authors.lastname: Tahan
authors.forename: Gagan
authors.initials: G
authors.lastname: Sood
authors.forename: Ciğdem
authors.initials: C
authors.lastname: Celikel
authors.forename: Nurdan
authors.initials: N
authors.lastname: Tözün
mesh_terms: D000818:Animals; D001652:Bile Ducts; D015415:Biomarkers / Q000097:blood; D004150:Dipeptidases / Q000097:blood*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D008026:Ligation; D008103:Liver Cirrhosis / Q000097:blood*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012044:Regression Analysis; D018709:Statistics, Nonparametric
chemical_list: D015415:Biomarkers; D007334:Insulin-Like Growth Factor I; D004150:Dipeptidases; C022449:proline dipeptidase