title: Compositional study on rice bran oil after lipase-catalyzed glycerolysis and solvent fractionations.
abstract: Rice bran oil (RBO) was modified through lipase-catalyzed glycerolysis. After 48 h reaction, the reactant (RBO-G, solved in hexane) containing 0.14 mg/mL of MAG, 0.19 mg/mL of DAG, and 0.93 mg/mL of TAG was obtained. Extending the reaction to 72 h resulted in 0.37 mg/mL of DAG with concomitant reduction in TAG (0.68 mg/mL). Two solvent fractionation methods, independent and sequential fractionation, were performed with acetone and hexane at 0, -8, -14, or -35 degrees C. The fraction with most unsaturated fatty acids (Sigma UFA) was liquid fraction from independent fractionation at -35 degrees C (-35 In) from hexane, showing 88.3%Sigma UFA content. Nevertheless, when yield (wt%) was considered, the highest amount of UFA was obtained from 0 In (liquid fraction from independent fractionation at 0 degrees C) with hexane, resulting in 82.3%Sigma UFA with 97.9 wt% recovery. Normal-phase HPLC was conducted for the compositional study of RBO-G. Overall, solid fractions from sequential fractionation at 0 degrees C (0 SeSo) and independent fractionation at -35 degrees C (-35 InSo) with hexane contained the high concentration of total MAG and DAG, ranging from 0.94 to 1.35 (mg/mL).
authors.affiliation: Department of Food Science Technology, The University of Georgia, Athens, GA 30602, USA.
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.forename: F
authors.initials: F
authors.lastname: Yu
authors.forename: P L
authors.initials: PL
authors.lastname: Vu
authors.forename: M S
authors.initials: MS
authors.lastname: Choi
authors.forename: C C
authors.initials: CC
authors.lastname: Akoh
authors.forename: K T
authors.initials: KT
authors.lastname: Lee
mesh_terms: D005591:Chemical Fractionation; D002623:Chemistry Techniques, Analytical; D004075:Diglycerides / Q000032:analysis / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism*; D050178:Monoglycerides / Q000032:analysis / Q000378:metabolism*; D010938:Plant Oils / Q000737:chemistry* / Q000378:metabolism*; D000073879:Rice Bran Oil; D012997:Solvents; D014280:Triglycerides / Q000032:analysis / Q000378:metabolism*
chemical_list: D004075:Diglycerides; D050178:Monoglycerides; D010938:Plant Oils; D012997:Solvents; D014280:Triglycerides; D008049:Lipase; D000073879:Rice Bran Oil