title: Multicentric Castleman disease is associated with polyfunctional effector memory HHV-8-specific CD8+ T cells.
abstract: Multicentric Castleman disease (MCD) is a devastating human herpesvirus 8 (HHV-8)-related lymphoproliferative disorder that occurs in immunocompromised persons. To determine the role of immune responses in MCD, we studied the frequency, antigenic repertoire, differentiation, and functional profile of HHV-8-specific CD8(+) T cells in MCD patients and in human immunodeficiency virus-coinfected asymptomatic HHV-8 carriers (AC). Screening CD8(+) T-cell responses with ELISpot interferon-gamma (IFN-gamma) assays using 56 peptides on 6 latent and lytic HHV-8 proteins showed that MCD and AC patients had responses of similar magnitude and antigenic repertoire and identified a new 10-mer human leukocyte antigen B7 CD8 epitope in K15. Intracellular IFN-gamma staining showed significantly more CD45RA(-)CCR7(-)CD27(-) CD8(+)IFN-gamma(+) cells (late phenotype) and significantly fewer CCR7(-)CD27(+)CD45RA(-) cells (early and intermediate phenotype) in MCD than in AC patients. This phenotypic shift was not found for Epstein-Barr virus-specific CD8(+) T cells tested as controls. HHV-8 viral loads were negatively correlated with early and intermediate effector memory cells. HHV-8-specific T cells were polyfunctional (secretion of IFN-gamma, tumor necrosis factor-alpha, macrophage inflammatory protein-1beta, and/or CD107a) in both MCD and AC patients. In conclusion, MCD is not associated with a lack of HHV-8-specific CD8(+) T cells or limitation of their functional profile. Their differentiation increases with HHV-8 viral load. These results offer new insight into the pathophysiology of MCD.
authors.affiliation: Laboratoire d'Immunologie Cellulaire, Hôpital Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Institut National de la Santé et de la Recherche Médicale, Université Pierre et Marie Curie Paris 6, Paris, France.
authors.forename: Amélie
authors.initials: A
authors.lastname: Guihot
authors.forename: Eric
authors.initials: E
authors.lastname: Oksenhendler
authors.forename: Lionel
authors.initials: L
authors.lastname: Galicier
authors.forename: Anne-Geneviève
authors.initials: AG
authors.lastname: Marcelin
authors.forename: Laura
authors.initials: L
authors.lastname: Papagno
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Bedin
authors.forename: Félix
authors.initials: F
authors.lastname: Agbalika
authors.forename: Nicolas
authors.initials: N
authors.lastname: Dupin
authors.forename: Jacques
authors.initials: J
authors.lastname: Cadranel
authors.forename: Brigitte
authors.initials: B
authors.lastname: Autran
authors.forename: Guislaine
authors.initials: G
authors.lastname: Carcelain
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000956:Antigens, Viral / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005871:Castleman Disease / Q000276:immunology* / Q000473:pathology; D002454:Cell Differentiation / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005260:Female; D006566:Herpesviridae Infections / Q000276:immunology* / Q000473:pathology; D019288:Herpesvirus 8, Human / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010641:Phenotype
chemical_list: D000956:Antigens, Viral; D000939:Epitopes