[Study on the ability of mammalian reovirus BYD1 to induce apoptosis and analysis of the structure of viral major membrane penetration protein involved in proapoptosis induction].
OBJECTIVE
To study a newly isolated domestic mammalian reovirus, BYD1, its ability to induce apoptosis analyze the three-dimensional structure of its major membrane penetration protein to predict its function in inducing apoptosis.


METHODS
HeLa cells infected with BYD1 reovirus were metered with flow cytometer (FCM) to quantify the ratio of apoptotic cells. The data were analyzed with Student's t-test to judge the ability of BYD1 strain to induce apoptosis. The primary sequence ranged from 582 to 675 per microliter protein of BYD1, T1L, T2J and T3D were aligned and compared. The three-dimensional comparative protein structure model of microliter protein was generated by homology-modeling pipeline SWISS MODEL was applied to annotate its secondary and tertiary structure.


RESULTS
BYD1 strain was verified with the ability to induce the apoptosis of HeLa cells. The 643-675 segment composing an alpha-helix showed major difference compared with prototype T2J.


CONCLUSIONS
The newly isolated reovirus BYD1 is an apoptosis inducing strain. The alpha-helix (residues 643 to 675) of microliter protein of BYD1 may play a key role to induce the proapoptotic activity of infected cells.
Duan
Hong-Yuan
HY
State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, China.
Zhang
Bao-Yuan
BY
Hu
Yi
Y
Song
Li-Hua
LH
Zhu
Hong
H
Duan
Qing
Q
D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D002454:Cell Differentiation; D005260:Female; D005434:Flow Cytometry; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D008958:Models, Molecular; D030102:Orthoreovirus, Mammalian / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D012087:Reoviridae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology; D054334:Viral Regulatory and Accessory Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*
D051017:Apoptosis Regulatory Proteins; D054334:Viral Regulatory and Accessory Proteins