hF-LANa, a human homologue of Derlin family, regulating the expression of cancer-related genes promotes NIH3T3 cell transformation.
hF-LANa, a member of Derlin family, is a putative proto-oncogene and has a direct role in oncogenic transformation. hF-LANa over-expressed NIH3T3 cells grow 20% quicker than parent cells. Especially, hF-LANa over-expression promotes anchorage-independent growth of NIH3T3 cells in soft agar and tumorigenesis in nude mice. Analysis by cDNA microarray finds 252 up-regulated genes and 354 down-regulated genes in hF-LANa over-expressed NIH3T3 cells including 19 oncogenes/proto-oncogenes and 13 tumor suppressor genes/putative tumor suppressors. These experiments show the oncogenic transformation role of hF-LANa both in vitro and in vivo, and highlight the genes that might be related to hF-LANa's oncogenic effects.
Hu
Yihong
Y
Laboratory of Molecular and Cellular Oncology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031, China.
Ying
Hao
H
Xu
Yonghua
Y
D000818:Animals; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000473:pathology; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D041681:NIH 3T3 Cells; D009374:Neoplasms, Experimental / Q000235:genetics / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D000090063:Proto-Oncogene Mas; D016160:Retinoblastoma Protein / Q000235:genetics; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation / Q000235:genetics; D051155:Wnt1 Protein / Q000235:genetics
C513305:DERL2 protein, human; C000715470:MAS1 protein, human; D008565:Membrane Proteins; D000090063:Proto-Oncogene Mas; D016160:Retinoblastoma Protein; D051155:Wnt1 Protein