FT-raman spectra of the border of infiltrating ductal carcinoma lesions.
OBJECTIVE
The characterization of the FT-Raman spectra of the borders of lesions is of great importance in guiding the surgeon during surgical intervention.


BACKGROUND
The main goals of this study were to investigate spectra of the borders of lesions of samples of infiltrating ductal carcinoma (IDC) and to determine the characteristics of these spectra.


METHODS
A total of 93 spectra were collected from five samples of healthy tissues and from 13 samples of IDC breast tissues using FT-Raman spectroscopy. Cluster analysis was used to separate the spectra into different groups. The results obtained from the statistical analysis were confirmed by a histopathological analysis.


RESULTS
The results showed that 17 out of the 67 spectra collected from the IDC samples demonstrated wide variety. The only significant difference between the peaks of the spectra of normal tissues and those of lesion borders is a peak at 538 cm(1) . This peak is related to disulfide bridges in cysteine, and it seems to be the main factor for the FT-Raman determination of the boundaries between healthy and pathological tissue.


CONCLUSIONS
These results serve as a foundation for future studies and application of Raman spectroscopy for optical diagnosis to guide biopsy and surgical intervention.
de Miranda Marzullo
Ana Carolina
AC
Laboratório de Espectroscopia Vibracional Biomédica, Instituto de Pesquisa e Desenvolvimento-IP&D, Universidade do Vale do Paraíba-UniVap, São José dos Campos, São Paulo, Brazil.
Neto
Osmar Pinto
OP
Bitar
Renata Andrade
RA
da Silva Martinho
Herculano
H
Martin
Airton Abrahão
AA
D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry* / Q000473:pathology*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D053844:Lasers, Solid-State; D013059:Spectrum Analysis, Raman