Expression of a lipid transfer protein in Escherichia coli and its phosphorylation by a membrane-bound calcium-dependent protein kinase.
Ha-AP10 is a basic antifungal peptide from sunflower seeds (Helianthus annuus antifungal peptide of 10 kDa) belonging to the family of plant lipid transfer proteins. We report here its expression in E. coli [Glutathione S-transferase (GST) system] and its phosphorylation by endogenous membrane-bound calcium-dependent protein kinases.
Martin
M L
ML
Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Casilla de Correo 1245, Mar del Plata, Argentina. marimart@mdp.edu.ar
Vidal
E Espinosa
EE
de la Canal
L
L
D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000378:metabolism; D052179:Antigens, Plant / Q000096:biosynthesis; D002352:Carrier Proteins / Q000096:biosynthesis*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis*; D011494:Protein Kinases / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D000935:Antifungal Agents; D052179:Antigens, Plant; D002352:Carrier Proteins; D010940:Plant Proteins; D011494:Protein Kinases; D011993:Recombinant Fusion Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C500581:Ha-AP10 protein, Helianthus annuus; C511920:lipid transfer proteins, plant