Comparison of mouse and rabbit model for the assessment of strong PGM-containing oil-based adjuvants.
Peptidoglycan monomer (PGM) is an adjuvant active molecule with potential for use in human and veterinary vaccine. PGM's action is short-lived in mammals hence its effects might be limited. Novel PGM-containing oil-based formulations have been developed recently by incorporation of PGM into Montanide ISA720 and ISA206 adjuvants with the aim to prolong and improve immunostimulating activities of PGM. In the present work we studied the efficacy of such novel adjuvant formulations using two different antigens, ovalbumin and snake venom, respectively. Novel formulations were also tested in two experimental models, mice and rabbits. In rabbits the incorporation of PGM into oil-based adjuvants led to overall improvement of antigen-specific IgG response. However, in the mouse model, under experimental conditions used, it was not possible to distinguish differences in antigen-specific IgG response among several strong oil-based adjuvant formulations.
Habjanec
Lidija
L
Research and Development Department, Institute of Immunology, Inc., Rockefellerova 10, HR-10000 Zagreb, Croatia. lhabjanec@imz.hr
Halassy
Beata
B
Vdović
Valerija
V
Balija
Maja Lang
ML
Tomasić
Jelka
J
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007114:Immunization / Q000379:methods*; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D008353:Mannitol / Q000031:analogs & derivatives* / Q000494:pharmacology; D051379:Mice; D008808:Mice, Inbred CBA; D023421:Models, Animal; D009829:Oleic Acids / Q000494:pharmacology*; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology*; D010457:Peptidoglycan / Q000494:pharmacology*; D011817:Rabbits; D012910:Snake Venoms / Q000276:immunology / Q000494:pharmacology*; D018709:Statistics, Nonparametric
D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D009829:Oleic Acids; D010457:Peptidoglycan; D012910:Snake Venoms; C477385:montanide ISA 51; D008353:Mannitol; D010047:Ovalbumin