Microemulsion electrokinetic chromatography for the separation of arctiin and arctigenin in Fructus Arctii and its herbal preparations.
A microemulsion electrokinetic chromatography method was used to separate arctiin and arctigenin in Fructus Arctii and its herbal preparations. The separation of arctiin and arctigenin was performed using a 1-butanol-SDS-ethyl acetate-water microemulsion in 10mM sodium tetraborate buffer. The analytes were baseline-resolved within 4 min. In the concentration range 5-500 microg/mL, the calibration curves reveal linear relationships between the peak area for each analyte and its concentration (correlation coefficients: 0.9993 for arctiin and 0.9998 for arctigenin). The method was applied to the analysis of arctiin and arctigenin in herbal preparations, and the recoveries were 98.7-103.1% for arctiin and 97.6-103.2% for arctigenin, respectively.
Lü
Wenjuan
W
Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
Chen
Yonglei
Y
Zhang
Yuxia
Y
Ding
Xiuping
X
Chen
Hongli
H
Liu
Mancang
M
D031170:Arctium / Q000737:chemistry; D001881:Borates; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D004655:Emulsions / Q000737:chemistry; D005663:Furans / Q000302:isolation & purification*; D005960:Glucosides / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D017705:Lignans / Q000302:isolation & purification*; D015203:Reproducibility of Results
D001881:Borates; D004365:Drugs, Chinese Herbal; D004655:Emulsions; D005663:Furans; D005960:Glucosides; D017705:Lignans; C010634:sodium borate; C077992:arctiin; C071942:arctigenin