title: Field application of the combination of neutralizing test and virus isolation, so-called spot test, to detect herds including cattle persistently infected with bovine viral diarrhea virus.
abstract: To detect herds including cattle persistently infected (PI) with bovine viral diarrhea virus (BVDV), application of the combination of neutralizing antibody detection and virus isolation, so-called spot test, were performed on sera of 3 calves selected from each of 26 farms. Nine farms were judged as positive because 64 or more antibody titers were detected from 2 or more calves or BVDV was isolated from one or more calves. PI cattle were detected from 8 of the 9 farms. The positive judgment on one farm was obtained only when the indicator virus used on the neutralizing test was genotypically identical with the isolate from the farm. These results suggest that the spot test can be effective in detecting herds with PI cattle and that the accuracy may be influenced by the genotypes of the indicator viruses.
authors.affiliation: Iwate Prefecture Central Livestock Hygiene Service Center, Japan. yoshihisa-seki@pref.iwate.jp
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Seki
authors.forename: Yukio M
authors.initials: YM
authors.lastname: Seimiya
authors.forename: Masato
authors.initials: M
authors.lastname: Motokawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyazaki
authors.forename: Masaru
authors.initials: M
authors.lastname: Konno
authors.forename: Gakuji
authors.initials: G
authors.lastname: Yaegashi
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D002353:Carrier State / Q000662:veterinary; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics / Q000302:isolation & purification*; D005838:Genotype; D009500:Neutralization Tests / Q000662:veterinary*; D010802:Phylogeny
chemical_list: D000914:Antibodies, Viral