Effects of emulsified octadecanic acids on gas production and cellulolysis by the rumen anaerobic fungus, Piromyces communis M014.
Responses of the rumen anaerobic fungus, Piromyces communis M014, to octadecanic long-chain fatty acids (LCFAs) were evaluated by measuring total and hydrogen gas productions, filter paper (FP) cellulose degradation and polysaccharidase enzyme activities. Octadecanic acids (stearic acid, C(18:0); oleic acid, C(18:1); linoleic acid, C(18:2) and linolenic acid, C(18:3)) were emulsified by ultrasonication under anaerobic conditions, and added to the medium at the level of 0.001%. When P. communis M014 was grown in culture with stearic and oleic acids, the cumulative gas production, FP cellulose digestion and enzyme activities were significantly (p<0.05) increased in the early incubation times relative to those for the control. However, the addition of linolenic acid inhibited all of the investigated parameters, including cellulose degradation, enzyme activities and gas production, up to 168h incubation. These results indicated that stearic and oleic acids tended to have stimulatory effects on fungal cellulolysis, whereas linolenic acid caused a significant (p<0.05) inhibitory effect on cellulolysis by the rumen fungus. The fungus, P. communis M014, can biohydrogenate C(18) unsaturated fatty acids to escape from their toxic effects. Therefore, in this study, the results indicated that the more highly the added C(18) LCFA to the fungal culture was unsaturated, the higher the inhibition of gas production and cellulase enzyme activity was.
Kim
Chang-H
CH
School of Animal Life and Environment Science, Hankyong National University, GRRC, Anseong 456-749, Republic of Korea.
Lee
Shin J
SJ
Ha
Jong K
JK
Kim
Wan Y
WY
Lee
Sung S
SS
D000641:Ammonia / Q000378:metabolism; D000693:Anaerobiosis; D000818:Animals; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism; D019787:Linoleic Acid / Q000494:pharmacology; D019301:Oleic Acid / Q000494:pharmacology; D020089:Piromyces / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology*; D013229:Stearic Acids / Q000494:pharmacology*; D014995:Xylosidases / Q000378:metabolism; D017962:alpha-Linolenic Acid / Q000494:pharmacology
D013229:Stearic Acids; D017962:alpha-Linolenic Acid; D019301:Oleic Acid; D019344:Lactic Acid; C031183:stearic acid; D000641:Ammonia; D006859:Hydrogen; D002482:Cellulose; D019787:Linoleic Acid; D014995:Xylosidases; D002480:Cellulase; C019826:carboxymethylcellulase