title: Antitumor activity of cytokine-induced killer cells against human lung cancer.
abstract: Lung cancer is the leading cause of cancer-related death among men and women in the world. Despite the aggressive treatment with surgery, radiation and chemotherapy, the long term survival for lung cancer patients remains low. In this study, the anti-tumor activity of cytokine-induced killer (CIK) cells against human lung cancer was evaluated in vitro and in vivo. Although CD3(+)CD56(+) CIK cells were rare in fresh human peripheral blood mononuclear cells, they could expand more than 1000-fold on day 14 in the presence of anti-CD3 antibody plus IL-2. At an effector-target cell ratio of 30:1, CIK cells destroyed 98% of NCI-H460 human lung cancer cells, which was determined by the (51)Cr-release assay. In addition, CIK cells at doses of 3 and 30 million cells per mouse inhibited 57% and 77% of NCI-H460 tumor growth in nude mouse xenograft assay, respectively. This study suggests that CD3(+)CD56(+) CIK cells may be used as an adoptive immunotherapy for patients with lung cancer.
authors.affiliation: Bioevaluation center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang, Chungbuk 363-883, South Korea.
authors.forename: Hwan Mook
authors.initials: HM
authors.lastname: Kim
authors.forename: Jaeseung
authors.initials: J
authors.lastname: Lim
authors.forename: Song-Kyu
authors.initials: SK
authors.lastname: Park
authors.forename: Jong Soon
authors.initials: JS
authors.lastname: Kang
authors.forename: Kiho
authors.initials: K
authors.lastname: Lee
authors.forename: Chang Woo
authors.initials: CW
authors.lastname: Lee
authors.forename: Ki Hoon
authors.initials: KH
authors.lastname: Lee
authors.forename: Mi-Jung
authors.initials: MJ
authors.lastname: Yun
authors.forename: Kyu-Hwan
authors.initials: KH
authors.lastname: Yang
authors.forename: Gyoonhee
authors.initials: G
authors.lastname: Han
authors.forename: Soon Woo
authors.initials: SW
authors.lastname: Kwon
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Kim
authors.forename: Sang-Bae
authors.initials: SB
authors.lastname: Han
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000032:analysis; D019002:CD56 Antigen / Q000032:analysis; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D007376:Interleukin-2 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000276:immunology*; D008175:Lung Neoplasms / Q000276:immunology / Q000628:therapy*; D051379:Mice
chemical_list: D017252:CD3 Complex; D019002:CD56 Antigen; D007376:Interleukin-2