Secoaggregatalactone-A from Lindera aggregata induces apoptosis in human hepatoma hep G2 cells.
A new secobutanolide, secoaggregatalactone A ( 1) was isolated from the leaves of Lindera aggregata. Results obtained from the cytotoxicity assay revealed that secoaggregatalactone A exhibited a noticeable cytotoxicity (EC (50) = 6.61 microg/mL; 22.1 microM) against the human hepatoma cell line (Hep G2 cell line). According to morphological observations, flow cytometric analysis, and DNA fragmentation analysis, it was proven that the cytotoxicity of secoaggregatalactone A on human cells was due to apoptosis. Moreover, based on the results from the protein expression assay and confocal laser scanning microscope observations, it is assumed that secoaggregatalactone A induced apoptosis through the mitochondria pathway by way of cleavage of Bit to release cytochrome C and activate caspases-9 and -3, and then degradation of PARP.
Lin
Chien-Tsong
CT
Department of Forestry, National Chung-Hsing University, Taichung, Taiwan.
Chu
Fang-Hua
FH
Chang
Shang-Tzen
ST
Chueh
Pin-Ju
PJ
Su
Yu-Chang
YC
Wu
King-Tsuen
KT
Wang
Sheng-Yang
SY
D015107:4-Butyrolactone / Q000031:analogs & derivatives; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004952:Esters / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D005434:Flow Cytometry; D006801:Humans; D027443:Lindera; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves
D000972:Antineoplastic Agents, Phytogenic; D004952:Esters; D010936:Plant Extracts; C526970:secoaggregatalactone A; D015107:4-Butyrolactone