Application of reverse micelle extraction process for amylase recovery using response surface methodology.
The effect of different process variables of reverse micelle extraction process like pH, addition of surfactant (AOT) concentration and potassium chloride (KCl) concentration on amylase recovery has been studied and analysed. Solid-state fermentation was used for the production of amylase enzyme. Response surface methodology (RSM) using central composite rotatable design (CCRD) was employed to analyse and optimize the enzyme extraction process. The regression analysis indicates that the effect of AOT concentration, and KCl concentration were significant, whereas the effect of pH was non-significant on enzyme recovery. For the maximum recovery of enzyme, the optimum operating condition for pH, AOT concentration (M) and KCl concentration were 10.43, 0.05 and 1.00, respectively. Under these optimal conditions, the enzyme recovery was 83.16%.
Bera
Manav B
MB
Biotechnology Research Laboratory, Department of Food Engineering and Technology, Sant Longowal Institute of Engineering and Technology, Longowal, 148106, Punjab, India.
Panesar
Parmjit S
PS
Panesar
Reeba
R
Singh
Bahadur
B
D000681:Amylases / Q000737:chemistry* / Q000302:isolation & purification*; D003102:Colloids / Q000737:chemistry* / Q000302:isolation & purification*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D003198:Computer Simulation; D008823:Micelles; D008956:Models, Chemical
D003102:Colloids; D008823:Micelles; D000681:Amylases