title: Diabetes insipidus and, partially, low anxiety-related behaviour are linked to a SNP-associated vasopressin deficit in LAB mice.
abstract: Following secretion from the posterior pituitary, the neuropeptide vasopressin (AVP) stimulates the kidney to retain water, and when released centrally it can contribute to anxiety- and depression-like behaviours. We hypothesized that CD1 mice bred for low trait anxiety (LAB) suffer from a deficit in AVP. Both osmotically stimulated peripheral secretion and intra-paraventricular nucleus (PVN) release of AVP were found decreased in LAB animals compared with normal anxiety (NAB) or high anxiety (HAB) controls. Consequently, in addition to their extreme non-anxiety, LAB mice showed signs of central diabetes insipidus (cDI), including increased fluid intake and reduced urine osmolality, as well as a pathological increase in plasma osmolality upon water deprivation. These cDI symptoms were attenuated by administration of a selective AVP V2 receptor agonist. A single nucleotide polymorphism (SNP) in exon 1 (C(+40)T) of the Avp gene of LAB animals causes an amino acid substitution in the signal peptide of the AVP precursor, and is likely to impair processing and trafficking of the precursor, as suggested by reduced axonal transport of AVP from the hypothalamic PVN, finally contributing to cDI symptoms and low trait anxiety. In an F2 panel, this SNP co-segregated with fluid intake and showed a partial contribution to low anxiety-related behaviour, indicated by its co-segregation with time spent on the open arms of the elevated plus-maze in a subset of F2 mice. Thus, the SNP-associated deficit in plasma and central AVP contributes to signs of cDI and, at least partially, to low trait anxiety, both features being typical of LAB animals.
authors.affiliation: Max Planck Institute of Psychiatry, 80804 Munich, Germany. kessler@mpipsykl.mpg.de
authors.forename: Melanie S
authors.initials: MS
authors.lastname: Kessler
authors.forename: Chris
authors.initials: C
authors.lastname: Murgatroyd
authors.forename: Mirjam
authors.initials: M
authors.lastname: Bunck
authors.forename: Ludwig
authors.initials: L
authors.lastname: Czibere
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Frank
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Jacob
authors.forename: Charlotte
authors.initials: C
authors.lastname: Horvath
authors.forename: Patrik
authors.initials: P
authors.lastname: Muigg
authors.forename: Florian
authors.initials: F
authors.lastname: Holsboer
authors.forename: Nicolas
authors.initials: N
authors.lastname: Singewald
authors.forename: Dietmar
authors.initials: D
authors.lastname: Spengler
authors.forename: Rainer
authors.initials: R
authors.lastname: Landgraf
mesh_terms: D000818:Animals; D001007:Anxiety / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D001127:Arginine Vasopressin / Q000172:deficiency*; D001522:Behavior, Animal; D003894:Deamino Arginine Vasopressin / Q000627:therapeutic use; D003919:Diabetes Insipidus / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004326:Drinking / Q000235:genetics; D005091:Exons; D005106:Exploratory Behavior / Q000502:physiology; D006490:Hemostatics / Q000627:therapeutic use; D008297:Male; D018782:Maze Learning / Q000502:physiology; D051379:Mice; D009994:Osmolar Concentration; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D010949:Plasma / Q000502:physiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011863:Radioimmunoassay / Q000379:methods; D014556:Urine / Q000502:physiology; D014868:Water Deprivation / Q000502:physiology
chemical_list: D006490:Hemostatics; D001127:Arginine Vasopressin; D003894:Deamino Arginine Vasopressin