High-performance liquid chromatography-electrospray mass spectrometry for the simultaneous determination of multiple active components in Sheng-Mai San, a prescription of traditional Chinese medicine.
An advanced and reliable HPLC-MS method was developed for the simultaneous quantification of eight active components (ginsenosides Rf, Rg(2), Rg(3), Rh(1) and Rh(2), gomisin A, methylophioponanone B and schizandrin) in Sheng-Mai San, a traditional Chinese medicine. The elution of multiple components was performed using a C(18) column with stepwise gradient elution. The detection of individual analytes was monitored by electrospray MS scanning from 300 to 1000 m/z in the positive ion mode, with the limits of detection of these components ranging from 0.06 to 1 microg/mL at a signal-to-noise ratio of > or =5. The intra- and inter-day accuracies ranged from 95.1 to 104.4%, and the overall precision was less than 9.3%. The recoveries of the analytes were > or =96.6%. The method was validated and found suitable for the determination of active components present in Sheng-Mai San preparation.
Chang
Yi-Wei
YW
Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli 350, Taiwan, Republic of China.
Yao
Hsien-Tsung
HT
Chien
Du-Shieng
DS
Yeh
Teng-Kuang
TK
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007529:Isoflavones / Q000032:analysis; D017705:Lignans / Q000032:analysis; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D012503:Saponins / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D017705:Lignans; D012503:Saponins; C009252:fructus schizandrae, radix ginseng, radix ophiopogonis drug combination