title: Intermediate frequency magnetic fields do not have mutagenic, co-mutagenic or gene conversion potentials in microbial genotoxicity tests.
abstract: We used bacterial mutation and yeast genotoxicity tests to evaluate the effects of intermediate frequency (IF; 2 kHz, 20 kHz and 60 kHz) magnetic fields (MFs) on mutagenicity, co-mutagenicity and gene conversion. We constructed a Helmholtz type exposure system that generated vertical and sinusoidal IF MFs, such as 0.91 mT at 2 kHz, 1.1 mT at 20 kHz and 0.11 mT at 60 kHz. Mutagenicity, co-mutagenicity and gene conversion assays were performed for each of the three MF exposure conditions. Mutagenicity testing was performed in four strains of Salmonella typhimurium (TA98, TA100, TA1535 and TA1537) and two strains of Escherichia coli (WP2 uvrA and WP2 uvrA/pKM101) to cover a wide spectrum of point mutations. For co-mutagenicity tests, we used four sensitive test strains (TA98, TA100, WP2 uvrA and WP2 uvrA/pKM101) with five chemical mutagens (t-butyl hydroperoxide (BH, a hydroxyl free radical precursor), 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide (AF2) and N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG, DNA reactive reagents), benz[a]pyrene (BaP) and 2-aminoanthracene (2AA, DNA reactive promutagens). Gene conversion testing was performed in the yeast test strain, Saccharomyces cerevisiae XD83. We also examined the effects on the repair process of DNA damage by UV irradiation. No statistically significant effects were observed between exposed and control groups in any of the genotoxicity tests, indicating that the IF MFs (0.91 mT at 2 kHz, 1.1 mT at 20 kHz or 0.11 mT at 60 kHz) do not have mutagenic or co-mutagenic potentials for the chemical mutagens tested under these experimental conditions. Our findings also indicate that these IF MFs do not induce gene conversion or affect the repair process of DNA damage in eukaryotic cells.
authors.affiliation: EMF Environment Sector, Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry, Abiko-City, Chiba, Japan. nakasono@criepi.denken.or.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nakasono
authors.forename: Masateru
authors.initials: M
authors.lastname: Ikehata
authors.forename: Minori
authors.initials: M
authors.lastname: Dateki
authors.forename: Sachiko
authors.initials: S
authors.lastname: Yoshie
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Shigemitsu
authors.forename: Tadashi
authors.initials: T
authors.lastname: Negishi
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000528:radiation effects; D005785:Gene Conversion / Q000235:genetics / Q000528:radiation effects*; D008280:Magnetics; D009152:Mutagenicity Tests / Q000379:methods; D009154:Mutation / Q000528:radiation effects*; D017354:Point Mutation / Q000187:drug effects; D012486:Salmonella typhimurium / Q000235:genetics / Q000528:radiation effects; D015003:Yeasts / Q000235:genetics / Q000528:radiation effects