title: Detection of human papillomavirus type 31-neutralizing antibodies from naturally infected patients by an assay based on intracellular assembly of luciferase-expressing pseudovirions.
abstract: The aim of this study was to develop a highly sensitive human papillomavirus type 31 (HPV31) neutralization assay based on the production of pseudovirions carrying luciferase. Neutralizing antibodies against HPV31 were investigated in a set of HPV31 monoclonal antibodies and in women with evidence of HPV31 infection. Neutralizing antibodies were detected in 78% of subjects with a positive enzyme-linked immunosorbent assay.
authors.affiliation: INSERM U618, Tours, France.
authors.forename: Maxime J J
authors.initials: MJ
authors.lastname: Fleury
authors.forename: Antoine
authors.initials: A
authors.lastname: Touzé
authors.forename: Silvia
authors.initials: S
authors.lastname: de Sanjosé
authors.forename: F Xavier
authors.initials: FX
authors.lastname: Bosch
authors.forename: Joellen
authors.initials: J
authors.lastname: Klaustermeiyer
authors.forename: Pierre
authors.initials: P
authors.lastname: Coursaget
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007313:Insecta; D008156:Luciferases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D009500:Neutralization Tests; D027383:Papillomaviridae / Q000276:immunology* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D014771:Virion / Q000201:enzymology* / Q000235:genetics; D019065:Virus Assembly / Q000502:physiology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D008156:Luciferases