Halobacillus faecis sp. nov., a spore-forming bacterium isolated from a mangrove area on Ishigaki Island, Japan.
A Gram-positive, spore-forming, rod-shaped halophilic bacterial strain, IGA7-4T, was isolated from a mangrove area on Ishigaki Island (Japan), and was characterized taxonomically using a polyphasic approach. Strain IGA7-4T was strictly aerobic and non-motile and formed central endospores. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain IGA7-4T is affiliated to the genus Halobacillus, and exhibits sequence similarities of 99.6-98.0% to the type strains of Halobacillus species. Levels of DNA-DNA relatedness between strain IGA7-4T and the type strains of Halobacillus species were 9.5-46.6%. The DNA G+C content of strain IGA7-4T was 46.5 mol%. The cell-wall peptidoglycan type (Orn-Asp), major cellular fatty acids (anteiso-C15:0, anteiso-C17:0, iso-C15:0 and iso-C16:0) and quinone type (MK-7) of the isolate support its affiliation to the genus Halobacillus. On the basis of phylogenetic analysis, phenotypic characteristics and chemotaxonomic data, the isolate represents a novel species of the genus Halobacillus, for which the name Halobacillus faecis sp. nov. is proposed. The type strain is IGA7-4T (=MBIC08268T=IAM 15427T=KCTC 13121T).
An
Sun-Young
SY
Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, 1-1-1 Bunkyo-Ku, Tokyo 113-0032, Japan.
Kanoh
Kaneo
K
Marine Biotechnology Co. Ltd, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
Kasai
Hiroaki
H
Marine Biotechnology Co. Ltd, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
Goto
Keiichi
K
Microbiological and Analytical Group, Food Research Laboratories, Mitsui Norin Co. Ltd, 223-1 Miyahara, Fujieda, Shizuoka 426-0133, Japan.
Yokota
Akira
A
Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, 1-1-1 Bunkyo-Ku, Tokyo 113-0032, Japan.
D001406:Bacillaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D015373:Bacterial Typing Techniques; D004275:DNA, Ribosomal; D019015:Geologic Sediments / Q000382:microbiology*; D007564:Japan; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D043982:Rhizophoraceae / Q000254:growth & development*; D045483:Rivers / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013171:Spores, Bacterial / Q000502:physiology
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride