title: Early pregnancy diagnosis by serum progesterone and ultrasound in sheep carrying somatic cell nuclear transfer-derived pregnancies.
abstract: Early pregnancy diagnosis and monitoring play an important role following embryo transfer in sheep. The aims of the current study were to investigate (i) the pattern of serum progesterone profiles in sheep carrying somatic cell nuclear transfer (SCNT)-derived (clone) pregnancies, and (ii) the frequency of pregnancy loss during development following SCNT embryo transfer. Sheep SCNT embryos were made using standard nuclear transfer techniques. Day 7 embryos were surgically transferred to oestrus-synchronized recipients (n = 27). As a control, normal fertile ewes (n = 12) were bred by natural breeding. Serum was collected from all the ewes on the day of estrus (day 0 sample), 7 days post-estrus (day 7 sample) and 19 days post-estrus (day 19 sample) and every 10 days thereafter until lambing or pregnancy loss occurred. Serum progesterone (P4) was assessed using enzyme immunoassay. Pregnancy was confirmed by ultrasound scanning on day 35 of pregnancy followed by subsequent scanning every 10 days. In control ewes, pregnancy rate on day 35 was 83.3% (10/12), whereas in the ewes that received SCNT embryos, it was 22.2% (6/27; p < 0.05). The day 45 pregnancy rate in the control ewes was 83.3%, whereas in the SCNT embryo recipients it was 11.0% (p < 0.05). Hormone analysis revealed that SCNT embryo recipients exhibited a significantly lower P4 profiles at different time points in pregnancy compared to controls (p < 0.05). This study highlights the use of serum progesterone in combination with ultrasound for the investigation of embryo loss and crucial times during development of normal and SCNT embryos in sheep. Further, the serum P4 levels directly reflect the degree of placental development in these two groups.
authors.affiliation: Department of Biomedical Sciences, Ontario Veterinary College (OVC), University of Guelph, ON, Canada.
authors.forename: B
authors.initials: B
authors.lastname: Alexander
authors.forename: G
authors.initials: G
authors.lastname: Coppola
authors.forename: G F
authors.initials: GF
authors.lastname: Mastromonaco
authors.forename: E
authors.initials: E
authors.lastname: St John
authors.forename: E R
authors.initials: ER
authors.lastname: Reyes
authors.forename: D H
authors.initials: DH
authors.lastname: Betts
authors.forename: W A
authors.initials: WA
authors.lastname: King
mesh_terms: D000818:Animals; D004624:Embryo Transfer / Q000662:veterinary*; D005260:Female; D011237:Predictive Value of Tests; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000097:blood / Q000502:physiology*; D011374:Progesterone / Q000097:blood*; D012680:Sensitivity and Specificity; D012756:Sheep; D016216:Ultrasonography, Prenatal / Q000662:veterinary
chemical_list: D011374:Progesterone