title: Strain difference of cadmium accumulation by liver slices of inbred Wistar-Imamichi and Fischer 344 rats.
abstract: Strain difference in the accumulation of cadmium (Cd) by liver slices was examined in inbred Cd-resistant Wistar-Imamichi (WI) and Cd-sensitive Fischer 344 (F344) rats. The accumulation of Cd by liver slices of WI rats was significantly lower than that of F344 rats, suggesting strain-related differences in the transport of Cd into the liver cells of these two rat strains. In addition, a similar strain difference was observed in the accumulation of zinc (Zn) by liver slices from WI and F344 rats. Cd accumulation by F344 liver slices decreased when Zn was added to the medium in combination with Cd. Furthermore, in F344 liver slices, Zn accumulation was significantly decreased when Cd was added to the medium. These results suggest that the accumulation of Cd by the liver is probably mediated, at least in part, by Zn transport systems. However, we found no strain difference in hepatic ZnT3 or ZIP3 transcript levels between WI and F344 rats. Further work is in progress to identify the transporter that causes the strain differences in hepatic Cd accumulation seen with WI and F344 rats.
authors.affiliation: Faculty of Education, Kumamoto University, 2-40-1, Kurokami, Kumamoto 860-8555, Japan. hshimada@gpo.kumamoto-u.ac.jp
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shimada
authors.forename: Akira
authors.initials: A
authors.lastname: Yasutake
authors.forename: Takaomi
authors.initials: T
authors.lastname: Hirashima
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Takamure
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kitano
authors.forename: Michael P
authors.initials: MP
authors.lastname: Waalkes
authors.forename: Yorishige
authors.initials: Y
authors.lastname: Imamura
mesh_terms: D000818:Animals; D002104:Cadmium / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D027682:Cation Transport Proteins / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology; D006360:Heat-Shock Proteins / Q000378:metabolism; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D017208:Rats, Wistar; D000071456:Sequestosome-1 Protein; D013045:Species Specificity; D015032:Zinc / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D027682:Cation Transport Proteins; D006360:Heat-Shock Proteins; D000071456:Sequestosome-1 Protein; C491079:Sqstm1 protein, rat; C503697:zinc transporter 3, rat; C017800:zinc-binding protein; D002104:Cadmium; D015032:Zinc