title: Analysis of metformin dosage formulations by capillary electrophoresis at nano scale detection.
abstract: An inexpensive, rapid and reproducible capillary electrophoretic method has been developed and validated for the determination of metformin in pharmaceutical preparations. The method was developed utilizing a fused silica capillary (60 cm x 50 microm I.D.), phosphate buffer (50 mM, 3.0 pH)-acetonitrile (95:5, v/v) as background electrolyte (BGE), 20 kV applied voltage with UV detection at 254 nm and at a working temperature of 23 +/- 1 degrees C. Linearity was observed in the concentration range from 100 ng/L to 5 microg/L, with a correlation coefficient (R2) of 0.9998. The limits of detection and quantification achieved were 60 and 100 ng/mL, respectively. The recovery of metformin from pharmaceutical preparations was 99.1%. These validation parameters demonstrate the precision of the method and its suitability for the determination of metformin in pharmaceutical tablet formulations.
authors.affiliation: Department of Chemistry, Jamia Millia Islamia (A Central University), Jamia Nagar, New Delhi, India. drimran_ali@yahoo.com
authors.forename: Imran
authors.initials: I
authors.lastname: Ali
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Aboul-Enein
authors.forename: Vinod K
authors.initials: VK
authors.lastname: Gupta
mesh_terms: D019075:Electrophoresis, Capillary / Q000191:economics / Q000295:instrumentation / Q000379:methods; D016014:Linear Models; D008687:Metformin / Q000032:analysis*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions / Q000737:chemistry; D013607:Tablets
chemical_list: D004364:Pharmaceutical Preparations; D012996:Solutions; D013607:Tablets; D008687:Metformin