A dose-rate effect in single-particle electron microscopy.
A low beam intensity, low electron dose imaging method has been developed for single-particle electron cryo-microscopy (cryo-EM). Experiments indicate that the new technique can reduce beam-induced specimen movement and secondary radiolytic effects, such as "bubbling". The improvement in image quality, especially for multiple-exposure data collection, will help single-particle cryo-EM to reach higher resolution.
Chen
James Z
JZ
Howard Hughes Medical Institute, Rosenstiel Center-MS029, Brandeis University, 415 South Street, Waltham, MA 02545-9110, USA.
Sachse
Carsten
C
Xu
Chen
C
Mielke
Thorsten
T
Spahn
Christian M T
CM
Grigorieff
Nikolaus
N
D020285:Cryoelectron Microscopy / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D014027:Tobacco Mosaic Virus / Q000648:ultrastructure