Exposure of mouse oocytes to 1,2-propanediol during slow freezing alters the proteome.
OBJECTIVE
To examine the effect of exposure to 1,2-propanediol (PrOH) on the mammalian oocyte proteome.


METHODS
Experimental study.


METHODS
Research laboratory.


METHODS
Four- to five-week-old F1 (C57BL/6 x CBA/Ca) female mice.


METHODS
Analysis by time-of-flight mass spectrometry.


METHODS
Protein profiles of denuded mouse metaphase II (MII) oocytes.


RESULTS
Metaphase II oocytes exposed to 1.5 M PrOH for 20 minutes with or without seeding (i.e., equilibration, dehydration with or without seeding) exhibited significantly altered protein profiles in comparison to controls (i.e., 37 degrees C for 20 minutes and room temperature for 20 min). In total, 11 protein-biomarkers displayed profiles of statistically significantly lower abundance, whereas 8 protein-biomarkers were observed to be statistically significantly up-regulated. Protein profiles of MII oocytes maintained at room temperature for 20 minutes were more similar to those that were incubated for 20 minutes at 37 degrees C.


CONCLUSIONS
Analysis of the mouse MII oocyte at different stages of the slow-freezing protocol revealed a negative impact on oocyte physiology after PrOH exposure. In contrast, temperature changes per se (during cooling to room temperature) have limited effect on the oocyte proteome.
Katz-Jaffe
Mandy G
MG
Colorado Center for Reproductive Medicine, Englewood, Colorado 80113, USA.
Larman
Mark G
MG
Sheehan
Courtney B
CB
Gardner
David K
DK
D000818:Animals; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000009:adverse effects*; D005260:Female; D005615:Freezing; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D019946:Propylene Glycol / Q000009:adverse effects*; D020543:Proteome / Q000187:drug effects*; D013997:Time Factors
D003451:Cryoprotective Agents; D020543:Proteome; D019946:Propylene Glycol