title: Comparison of mouse and rabbit model for the assessment of strong PGM-containing oil-based adjuvants.
abstract: Peptidoglycan monomer (PGM) is an adjuvant active molecule with potential for use in human and veterinary vaccine. PGM's action is short-lived in mammals hence its effects might be limited. Novel PGM-containing oil-based formulations have been developed recently by incorporation of PGM into Montanide ISA720 and ISA206 adjuvants with the aim to prolong and improve immunostimulating activities of PGM. In the present work we studied the efficacy of such novel adjuvant formulations using two different antigens, ovalbumin and snake venom, respectively. Novel formulations were also tested in two experimental models, mice and rabbits. In rabbits the incorporation of PGM into oil-based adjuvants led to overall improvement of antigen-specific IgG response. However, in the mouse model, under experimental conditions used, it was not possible to distinguish differences in antigen-specific IgG response among several strong oil-based adjuvant formulations.
authors.affiliation: Research and Development Department, Institute of Immunology, Inc., Rockefellerova 10, HR-10000 Zagreb, Croatia. lhabjanec@imz.hr
authors.forename: Lidija
authors.initials: L
authors.lastname: Habjanec
authors.forename: Beata
authors.initials: B
authors.lastname: Halassy
authors.forename: Valerija
authors.initials: V
authors.lastname: Vdović
authors.forename: Maja Lang
authors.initials: ML
authors.lastname: Balija
authors.forename: Jelka
authors.initials: J
authors.lastname: Tomasić
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007114:Immunization / Q000379:methods*; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D008353:Mannitol / Q000031:analogs & derivatives* / Q000494:pharmacology; D051379:Mice; D008808:Mice, Inbred CBA; D023421:Models, Animal; D009829:Oleic Acids / Q000494:pharmacology*; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology*; D010457:Peptidoglycan / Q000494:pharmacology*; D011817:Rabbits; D012910:Snake Venoms / Q000276:immunology / Q000494:pharmacology*; D018709:Statistics, Nonparametric
chemical_list: D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D009829:Oleic Acids; D010457:Peptidoglycan; D012910:Snake Venoms; C477385:montanide ISA 51; D008353:Mannitol; D010047:Ovalbumin