title: CD44 and PTGS2 methylation are independent prognostic markers for biochemical recurrence among prostate cancer patients with clinically localized disease.
abstract: Up to 30% of men with clinically localized disease who receive radical prostatectomy develop a biochemical recurrence. Gene methylation in tumor tissue may distinguish men with aggressive cancer. This study evaluated methylation of GSTP1, RARb2, CD44 and PTGS2 with biochemical recurrence among 60 patients who underwent radical prostatectomy using logistic regression and Kaplan Meier time to event analysis. Methylation of GSTP1 and RARbeta2 was not associated with recurrence, however, CD44 and PTGS2 methylation were significant predictors. In multivariate models adjusting for Gleason grade, the methylation profile of CD44 and PTGS2 combined was an independent predictor of biochemical recurrence (associated with 9-fold increased risk). In addition, Kaplan Meier analysis showed CD44 and PTGS2 methylation was associated with shorter time to recurrence. CD44 and PTGS2 methylation may predict biochemical recurrence in prostate cancer patients undergoing radical prostatectomy and if validated in larger studies, may identify patients with aggressive cancer.
authors.affiliation: Genetics Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA. kw114v@nih.gov
authors.forename: Karen
authors.initials: K
authors.lastname: Woodson
authors.forename: Keith J
authors.initials: KJ
authors.lastname: O'Reilly
authors.forename: David E
authors.initials: DE
authors.lastname: Ward
authors.forename: Jack
authors.initials: J
authors.lastname: Walter
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Hanson
authors.forename: Elyse L
authors.initials: EL
authors.lastname: Walk
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Tangrea
mesh_terms: D000704:Analysis of Variance; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D016014:Linear Models; D008297:Male; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis* / Q000378:metabolism; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D018960:Hyaluronan Receptors; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human