Decomposition of myceliar matrix and extraction of chitosan from Gongronella butleri USDB 0201 and Absidia coerulea ATCC 14076.
Free chitosan, 2 g/100g mycelia from Gongronella butleri and 6.5 g/100g mycelia from Absidia coerulea were isolated by 1M NaOH at 45 degrees C for 13 h and 0.35 M acetic acid at 95 degrees C for 5 h. Both myceliar matrixes did not break down under these conditions. However, myceliar matrix could be decomposed by treatment with 11 M NaOH at 45 degrees C for 13 h and 0.35 M acetic acid at 95 degrees C for 5 h and then extracted the total chitosan, 8-9 g/100g mycelia from both fungi. According to these results, G. butleri has higher amount of complexed chitosan and A. coerulea has higher amount in free chitosan.
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Faculty of Chemistry, Materials and Bioengineering and HRC, Kansai University, Suita, Osaka 564-8680, Japan.
Stevens
Willem F
WF
Montet
Didier
D
Tokura
Seiichi
S
Tamura
Hiroshi
H
D000468:Alkalies; D048271:Chitosan / Q000737:chemistry* / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D009090:Mucorales / Q000737:chemistry* / Q000145:classification / Q000166:cytology*; D012972:Sodium Hydroxide; D012995:Solubility; D013055:Spectrophotometry, Infrared
D000468:Alkalies; D012972:Sodium Hydroxide; D048271:Chitosan