title: Chitinase inhibitor allosamidin promotes chitinase production of Streptomyces generally.
abstract: Allosamidin is a family 18 chitinase inhibitor produced by Streptomyces. In its producing strain, Streptomyces sp. AJ9463, allosamidin promotes production of the family 18 chitinase originated from chi65 in a chitin medium through the two-component regulatory system encoded by chi65R and chi65S, which were present at the 5'-upstream region of chi65. In this study, we showed generality of the allosamidin's effect. Allosamidin enhanced production of the family 18 chitinases originated from chi65h of Streptomyces halstedii MF425, another allosamidin producer, chiC of Streptomyces coelicolor A3(2) and chiIII of Streptomyces griseus. All the three chitinase genes had high homology to chi65 and two genes homologous to chi65S and chi65R were present at their 5'-upstream regions. When allosamidin's effect was tested with six Streptomyces strains randomly isolated from soil, allosamidin enhanced chitinase production of all strains. All six strains possessed a set of three genes homologous to chi65, chi65S and chi65R. Analysis of 16S rDNA indicated that allosamidin-sensitive strains are distributed widely in Streptomyces. These observations suggested that allosamidin can affect the common regulatory system for production of a chitinase with a two-component regulatory system in Streptomyces.
authors.affiliation: Department of Applied Biological Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Suzuki
authors.forename: Eiyu
authors.initials: E
authors.lastname: Nakanishi
authors.forename: Keiko
authors.initials: K
authors.lastname: Furihata
authors.forename: Katsushiro
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsujibo
authors.forename: Takeshi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: Sueharu
authors.initials: S
authors.lastname: Horinouchi
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Nagasawa
authors.forename: Shohei
authors.initials: S
authors.lastname: Sakuda
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002688:Chitinases / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000235:genetics; D012988:Soil Microbiology; D013302:Streptomyces / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D014312:Trisaccharides / Q000494:pharmacology*
chemical_list: D004269:DNA, Bacterial; D004791:Enzyme Inhibitors; D014312:Trisaccharides; C054851:allosamidin; D002688:Chitinases; D000117:Acetylglucosamine