How do TTX and AP5 affect the post-recovery neuronal network activity synchronization?
A lot of methods were created in last decade for the spatio-temporal analysis of multi-electrode array (MEA) neuronal data sets. The greater part of these methods does not consider the network as a whole but performs an analysis channel by channel. In this paper we illustrate how a very simple approach that considers the total network activity, is able to show interesting neuronal network features. In particular we perform two different analyses: a connectivity examination studying networks at different days in vitro and an analysis of the long period effects of the administration of two common neuro-active drugs, i.e. TTX and AP5. Our analysis is performed considering burst topology, i.e. cataloguing network bursts as Global (if they involve more than the 25% of the MEA channels) or Local (if less that 25%). This division allows, in the first analysis, to understand the network connectivity (increasing from div 1 to 6) and decreasing till reaching a plateau (from div 6 to 10). The second analysis highlights a substantial difference between the long period effects of TTX and AP5. While TTX induces a massive Global activity explosion, sign of a prolonged inhibitory synapse depression, AP5 shows only a modest Local activity increase, mark of the low effect of NMDA receptors on a mature neuronal network without inputs.
Esposti
Federico
F
Politecnico di Milano technical University, Milan, Italy. federico.esposti@polimi.it
Signorini
Maria Gabriella
MG
Lamanna
Jacopo
J
Gullo
Francesca
F
Wanke
Enzo
E
D015763:2-Amino-5-phosphonovalerate / Q000008:administration & dosage*; D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000222:Adaptation, Physiological / Q000187:drug effects / Q000502:physiology; D000779:Anesthetics, Local / Q000008:administration & dosage; D000818:Animals; D001683:Biological Clocks / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000502:physiology*; D003198:Computer Simulation; D003344:Cortical Synchronization / Q000379:methods; D018691:Excitatory Amino Acid Antagonists / Q000008:administration & dosage; D008959:Models, Neurological; D009415:Nerve Net / Q000187:drug effects / Q000502:physiology*; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D013779:Tetrodotoxin / Q000008:administration & dosage*
D000779:Anesthetics, Local; D018691:Excitatory Amino Acid Antagonists; D013779:Tetrodotoxin; D015763:2-Amino-5-phosphonovalerate