[Effect of TCM treatment for purging fire, removing toxic substance, relieving stagnation and eliminating phlegm on cytoplasmic free calcium and intercellular adhesion molecule-1 in rats with intracerebral hemorrhage].
OBJECTIVE
To study the effects and possible mechanisms of TCM treatment for purging fire, removing toxic substance, relieving stagnation and eliminating phlegm on cytoplasmic free calcium ([Ca2+]i) and intercellular adhesion molecule-1 (ICAM-1) in rats with intracerebral hemorrhage (ICH).


METHODS
Adult SD rats were randomly divided into six groups: the normal group, the operated group, the model group, the Naoqing Decoction (NQD) low- and high-dose groups, and the Qingkailing Injection (QKL) group. ICH model was induced by injecting collagenase into right caudate nucleus, level of [Ca2+]i was determined by fluorescent tracing assay, and the expression of ICAM-1 was determined using immunohistochemical technique.


RESULTS
High- and low-dose of NQD and QKL could significantly antagonize the collagenase injection induced increase of [Ca2+]i (P < 0.01), inhibit the expression of ICAM-1 and shorten its sustainable time.


CONCLUSIONS
NQD plays a role in protecting neuron by inhibiting calcium overload, regulating cytokines network, and inhibiting inflammatory reaction through postponing the release of cytokines and inflammatory mediators.
Xianyu
Kai-pu
KP
Department of Traditional Chinese Medicine, Wuhan Chinese and Western Hospital, Wuhan. hbxykp@21cn.com
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002543:Cerebral Hemorrhage / Q000188:drug therapy* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008297:Male; D008516:Medicine, Chinese Traditional / Q000379:methods*; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D018799:Intercellular Adhesion Molecule-1; D002118:Calcium