[Effect of human oviductal embryotrophic factors on gene expression of mouse preimplantation embryos].
OBJECTIVE
To investigate the effect of embryotrophic factors (ETF) from human oviductal cells on gene expression of mouse early developmental embryos and discuss the role of fallopian tube in early development of embryos.


METHODS
ETF was isolated from conditioned medium of human oviductal cell line by sequential liquid chromatographic systems. Mouse embryos were treated by ETF in vitro. Using differential display RT-PCR, the gene expression of embryos treated by ETF was compared with embryos without ETF treatment. The differentially expressed genes were separated, re-amplified, cloned and sequenced.


RESULTS
Gene expression profiles of embryos with ETF treatment was different from embryos without this treatment. Eight differentially expressed genes were cloned and sequenced. These genes functioned in RNA degradation, synthesis, splicing, protein trafficking, cellular differentiation and embryo development.


CONCLUSIONS
Embryotrophic factors from human oviductal cells affect gene expression of early developmental embryos. The human oviductal cells play wide roles in early developmental stages of embryos.
Yao
Yuan-Qing
YQ
Department of Obstetrics and Gynecology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. yqyao@126.com
Lee
Kai-Fai
KF
Xu
Jia-Seng
JS
Ho
Pak-Chung
PC
Yeung
Shu-Biu
SB
D019883:Alkyl and Aryl Transferases / Q000235:genetics; D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000187:drug effects* / Q000235:genetics; D005187:Fallopian Tubes / Q000166:cytology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006023:Glycoproteins / Q000235:genetics; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017077:Culture Media, Conditioned; D006023:Glycoproteins; D006133:Growth Substances; D019883:Alkyl and Aryl Transferases; C075607:Rab geranylgeranyltransferase