title: The N-terminal domain of 2',3'-cyclic nucleotide 3'-phosphodiesterase harbors a GTP/ATP binding site.
abstract: The interaction between 2',3'-cyclic nucleotide 3'-phosphodiesterase and guanine/adenine nucleotides was investigated. The binding of purine nucleotides to 2',3'-cyclic nucleotide 3'-phosphodiesterase was revealed by both direct and indirect methods. In fact, surface plasmon resonance experiments, triphosphatase activity measurements, and fluorescence experiments revealed that 2',3'-cyclic nucleotide 3'-phosphodiesterase binds purine nucleotide triphosphates with an affinity higher than that displayed for diphosphates; on the contrary, the affinity for both purine monophosphates and pyrimidine nucleotides was negligible. An interpretation of biological experimental data was achieved by a building of 2',3'-cyclic nucleotide 3'-phosphodiesterase N-terminal molecular model. The structural elements responsible for nucleotide binding were identified and potential complexes between the N-terminal domain of CNP-ase and nucleotide were analyzed by docking simulations. Therefore, our findings suggest new functional and structural property of the N-terminal domain of CNPase.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università di Salerno, Via Ponte Don Melillo, Fisciano (SA) 84084, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Stingo
authors.forename: Mariorosario
authors.initials: M
authors.lastname: Masullo
authors.forename: Eugenia
authors.initials: E
authors.lastname: Polverini
authors.forename: Chiara
authors.initials: C
authors.lastname: Laezza
authors.forename: Immacolata
authors.initials: I
authors.lastname: Ruggiero
authors.forename: Rosaria
authors.initials: R
authors.lastname: Arcone
authors.forename: Enrica
authors.initials: E
authors.lastname: Ruozi
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Dal Piaz
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Malfitano
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: D'Ursi
authors.forename: Maurizio
authors.initials: M
authors.lastname: Bifulco
mesh_terms: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001665:Binding Sites / Q000502:physiology; D003198:Computer Simulation; D008958:Models, Molecular; D011485:Protein Binding / Q000502:physiology; D011685:Purine Nucleotides / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D013329:Structure-Activity Relationship; D013379:Substrate Specificity / Q000502:physiology
chemical_list: D011685:Purine Nucleotides; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases