title: A recombinant triblock protein polymer with dispersant and binding properties for digital printing.
abstract: A structured triblock protein was designed to explore the potential of engineered peptides to function as high-performance ink dispersants and binders. The protein consists of three functional elements, including a pigment binding domain, a hydrophilic linker, and a printing surface binding domain. To construct such a chimeric protein, a carbon black binding peptide, FHENWPS, and a cellulose binding peptide, THKTSTQRLLAA, were identified from phage display libraries through biopanning, based on their strong and specific binding affinities to carbon black and cellulose. They were used as carbon black and cellulose binding domains, respectively, in a recombinant triblock protein. A linker sequence, PTPTPTPTPTPTPTPTPTPTPTP, was adapted from endoglucanase A of the bacterium Cellulomonas fimi, as a small, rigid, and hydrophilic interdomain linker. When incorporated into the triblock structure between the carbon black and cellulose binding sequences, the linker sufficiently isolates these two elements and allows dual binding activity. The structured triblock protein was shown to disperse carbon black particles and attach it to paper surfaces. Thus, the utility of structured proteins having useful dispersant and binding properties for digital printing inks was demonstrated.
authors.affiliation: DuPont Central Research and Development, Experimental Station, Wilmington, DE 19880-0402, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Qi
authors.forename: John P
authors.initials: JP
authors.lastname: O'Brien
authors.forename: Jianjun
authors.initials: J
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D002482:Cellulose / Q000737:chemistry; D007281:Ink; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011327:Printing; D011994:Recombinant Proteins / Q000737:chemistry*
chemical_list: D010455:Peptides; D011108:Polymers; D011994:Recombinant Proteins; C527837:TPB 101 protein; C527836:TPB 201 protein; D002482:Cellulose