title: Programmed cell death 4 (PDCD4) is an important functional target of the microRNA miR-21 in breast cancer cells.
abstract: MicroRNAs are emerging as important regulators of cancer-related processes. The miR-21 microRNA is overexpressed in a wide variety of cancers and has been causally linked to cellular proliferation, apoptosis, and migration. Inhibition of mir-21 in MCF-7 breast cancer cells causes reduced cell growth. Using array expression analysis of MCF-7 cells depleted of miR-21, we have identified mRNA targets of mir-21 and have shown a link between miR-21 and the p53 tumor suppressor protein. We furthermore found that the tumor suppressor protein Programmed Cell Death 4 (PDCD4) is regulated by miR-21 and demonstrated that PDCD4 is a functionally important target for miR-21 in breast cancer cells.
authors.affiliation: Biotech Research and Innovation Centre, Bioinformatics Centre, University of Copenhagen, DK-2200 N Copenhagen, Denmark.
authors.forename: Lisa B
authors.initials: LB
authors.lastname: Frankel
authors.forename: Nanna R
authors.initials: NR
authors.lastname: Christoffersen
authors.forename: Anders
authors.initials: A
authors.lastname: Jacobsen
authors.forename: Morten
authors.initials: M
authors.lastname: Lindow
authors.forename: Anders
authors.initials: A
authors.lastname: Krogh
authors.forename: Anders H
authors.initials: AH
authors.lastname: Lund
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D002455:Cell Division; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007700:Kinetics; D035683:MicroRNAs / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D051017:Apoptosis Regulatory Proteins; D035683:MicroRNAs; C114487:PDCD4 protein, human; D012334:RNA, Neoplasm; D016601:RNA-Binding Proteins