title: Expression of an ovarian cancer anti-idiotype antibody (6B11VLVHCH3) in Chinese hamster ovary (CHO) cells with improved immunoactivity and stability over proteins expressed in prokaryotic cells.
abstract: The 6B11VLVHCH3 is an anti-idiotype antibody to ovarian cancer. It can mimic the ovarian cancer-associated antigen OC166-9. We have previously expressed it in Escherichia coli. We now express the 6B11VLVHCH3 in a Chinese hamster ovary (CHO) cell system. The 6B11VLVHhCH3 sequence was amplified from the prokaryotic vector and cloned into the eukaryotic expression vector pSecTag2. The construct was transfected into CHO cells. Then the transfected monoclonal CHO cells were sequentially adapted to growth in serum-free medium. Functional differences between the antibody produced in E. coli or CHO cells were assessed. The antibody produced by CHO cells was able to both mimic ovarian cancer antigen and retain FC region functionality. Importantly, the antigen mimicry activity of 6B11VLVHhCH3 produced by CHO cells is approximately 20 times higher than antibody expressed by E. coli. This method of expression provides a safer and higher quality antibody for clinical drug development.
authors.affiliation: Gynecological Oncology Center, Peking University People's Hospital, Beijing, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Shi
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Chang
authors.forename: Jie
authors.initials: J
authors.lastname: Feng
authors.forename: Yexia
authors.initials: Y
authors.lastname: Cheng
authors.forename: Hongyan
authors.initials: H
authors.lastname: Cheng
authors.forename: Huifang
authors.initials: H
authors.lastname: Guo
authors.forename: Xue
authors.initials: X
authors.lastname: Ye
authors.forename: Heng
authors.initials: H
authors.lastname: Cui
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism*; D000912:Antibodies, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D000951:Antigens, Neoplasm / Q000276:immunology* / Q000378:metabolism; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004926:Escherichia coli; D005260:Female; D008969:Molecular Sequence Data; D010051:Ovarian Neoplasms / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm