p38MAP Kinase activity is required for human primary adipocyte differentiation.
Little is known about the role of p38MAPK in human adipocyte differentiation. Here we showed that p38MAPK activity increases during human preadipocytes differentiation. Pharmacological inhibition of p38MAPK during adipocyte differentiation of primary human preadipocytes markedly reduced triglycerides accumulation and adipocyte markers expression. Cell cycle arrest or proliferation was not affected by p38MAPK inhibition. Although induction of C/EBPbeta was not altered by the p38MAPK inhibitor, its phosphorylation on Threonine(188) was decreased as well as PPARgamma expression. These results indicate that p38MAPK plays a positive role in human adipogenesis through regulation of C/EBPbeta and PPARgamma factors.
Aouadi
M
M
INSERM, U 568, IFR50, F-06107, Nice, France. myriam.aouadi@umassmed.edu
Jager
J
J
Laurent
K
K
Gonzalez
T
T
Cormont
M
M
Binétruy
B
B
Le Marchand-Brustel
Y
Y
Tanti
J-F
JF
Bost
F
F
D017667:Adipocytes / Q000166:cytology* / Q000201:enzymology*; D050156:Adipogenesis / Q000502:physiology; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D047495:PPAR gamma / Q000378:metabolism; D010766:Phosphorylation; D015398:Signal Transduction; D013912:Threonine / Q000235:genetics / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D022782:CCAAT-Enhancer-Binding Protein-beta; D007093:Imidazoles; D047495:PPAR gamma; D013912:Threonine; D048051:p38 Mitogen-Activated Protein Kinases; C408604:2-(4-nitrophenyl)-4-(4-fluorophenyl)-5-(4-pyridinyl)-1H-imidazole