title: Development of a convenient in vitro fertilization method using interspecific hybrids between Oryzias latipes and Oryzias curvinotus.
abstract: Medaka is a small Asian freshwater teleost and has been an excellent model for fertilization studies for more than 50 years. Therefore, experimental procedures for in vitro fertilization (IVF) and cryopreservation of sperm are well established. In contrast, since the eggs or early embryos can not be cryopreserved, many females are killed to obtain unfertilized eggs for IVF. Recent progress in genomics is establishing medaka as a new model animal in functional genomics, and numerous mutant and transgenic strains have been established and stored as frozen sperm. Accumulated preserved resources require a simple and reliable recovery method for IVF. In this paper, we describe a method for obtaining a large number of unfertilized eggs without killing females, using sterile interspecific hybrids between Oryzias latipes and O. curvinotus. However, there is no report about the normality of offspring that were obtained by IVF using unfertilized eggs spawned in mating with the sterile hybrid male. In this paper, we have confirmed the reliability of the method regarding the influences on the next generation and also assessed conditions for efficient collection of unfertilized eggs. The method would be useful not only for fertilization studies but also for keeping transgenics and mutants, including a mutant library for a reverse genetic approach.
authors.affiliation: Radiation Biology Center, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan. y-kamei@house.rbc.kyoto-u.ac.jp
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kamei
authors.forename: Junji
authors.initials: J
authors.lastname: Itou
authors.forename: Shoji
authors.initials: S
authors.lastname: Oda
authors.forename: Mami
authors.initials: M
authors.lastname: Masui
authors.forename: Jin-Hyeong
authors.initials: JH
authors.lastname: Kim
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yuba
authors.forename: Masato
authors.initials: M
authors.lastname: Kinoshita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mitani
authors.forename: Takeshi
authors.initials: T
authors.lastname: Todo
mesh_terms: D000818:Animals; D015925:Cryopreservation; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006824:Hybridization, Genetic; D008297:Male; D009990:Oryzias / Q000235:genetics* / Q000502:physiology; D010063:Ovum; D012662:Semen Preservation / Q000379:methods