title: Identification and manipulation of antigen specific T-cells with artificial antigen presenting cells.
abstract: T-cells specific for a particular antigen represent a small percentage of the overall T-cell population. Detecting the presence of antigen specific T-cells in patients, animal models or populations of cultured cells has presented a challenge to researchers. The T-cell capture method described here utilizes a truly artificial method of antigen presentation and requires only 50,000 cells for the detection of the major histomcompatibility complex (MHC) class II and antigen restricted T-cells. With this method, liposomes, prepared with readily available materials, are loaded with neutravidin "rafts" comprised of MHC/peptide complexes, anti-CD28, a costimulatory molecule, and anti-LFA-1, an adhesion molecule. These artificial APCs are easily manipulated to include any MHC, antibodies to cell surface markers and/or costimulatory signals of interest thereby enabling not only T-cell identification but also the manipulation of mechanisms of T-cell activation.
authors.affiliation: Department of Medicine, University of California San Diego, La Jolla, USA.
authors.forename: Eva
authors.initials: E
authors.lastname: Koffeman
authors.forename: Elissa
authors.initials: E
authors.lastname: Keogh
authors.forename: Mark
authors.initials: M
authors.lastname: Klein
authors.forename: Berent
authors.initials: B
authors.lastname: Prakken
authors.forename: Salvatore
authors.initials: S
authors.lastname: Albani
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods; D005802:Genes, MHC Class II; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000378:metabolism; D010455:Peptides / Q000276:immunology / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D000941:Antigens; D008055:Lipids; D008081:Liposomes; D010455:Peptides