Simultaneous determination of active ingredients in ethnomedicine Gaultheria leucocarpa var. yunnanensis and its medicinal preparation by capillary electrophoresis with electrochemical detection.
A simple and rapid capillary electrophoresis (CE) with electrcochemical detection (ED) method has been established for the simultaneous determination of seven active ingredients in the stems and roots of Gaultheria leucocarpa var. yunnanensis and its medicinal preparation, including (+)-catechin, rutin, gentisic acid, vallinic acid, salicylic acid, quercetin, and protocatechuic acid. The effects of working potential, pH, and concentration of running buffer, separation voltage, and injection time on CE-ED are systematically investigated. Under the optimum conditions, the seven analytes could be completely separated within 23 min in a borax running buffer (pH 8.7). A good linear relationship is obtained over three orders of magnitude with detection limits (signal-to-noise ratio=3) ranging from 5x10(-8) g/mL to 3x10(-7) g/mL for the analytes. The proposed method is successfully used in the analysis of real samples after a relatively simple extraction procedure, and the assay results are satisfactory.
Zeng
Yikun
Y
Department of Chemistry, East China Normal University, Shanghai 200062, China.
Tang
Zhuxing
Z
Wang
Qingjiang
Q
He
Pingang
P
Fang
Yuzhi
Y
D002392:Catechin / Q000032:analysis; D004563:Electrochemistry / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D031282:Gaultheria / Q000737:chemistry*; D005841:Gentisates / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D062385:Hydroxybenzoates / Q000032:analysis; D028321:Plant Preparations / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D011794:Quercetin / Q000032:analysis; D015203:Reproducibility of Results; D012431:Rutin / Q000032:analysis; D020156:Salicylic Acid / Q000032:analysis; D012680:Sensitivity and Specificity; D014641:Vanillic Acid / Q000031:analogs & derivatives / Q000032:analysis
C525112:1,3-dihydroxy-4-methoxybenzoic acid; D005841:Gentisates; D062385:Hydroxybenzoates; D028321:Plant Preparations; C009091:protocatechuic acid; D012431:Rutin; D002392:Catechin; D011794:Quercetin; D014641:Vanillic Acid; D020156:Salicylic Acid; C010925:2,5-dihydroxybenzoic acid