Molecularly imprinted polymer-based solid-phase extraction combined with molecularly imprinted polymer-based sensor for detection of uric acid.
The development of an easy-to-use, rapid, robust and inexpensive technique is required which can measure the basal concentration of uric acid (UA) lower than 1.0 x 10(-7)M ( approximately 0.017 mgL(-1)) in biological samples to attend the problem of hypouricemia. In the present work an artificial receptor for UA, silica gel-bonded molecularly imprinted polymer (MIP), was used as a sorbent for molecularly imprinted solid-phase extraction (MISPE) in column chromatography. The use of a sensor based on a MIP-modified hanging mercury drop electrode (HMDE), as reported from our laboratory, could estimate UA with detection limit as low as 0.024 mgL(-1) under the optimized conditions of differential pulse, cathodic stripping voltammetric (DPCSV) measurement. However, in the current investigation, with the use of the combination of MISPE followed by detection with a MIP-based HMDE sensor, the minimum detectable concentration could go down to 0.0008 mgL(-1) (RSD=0.63%, S/N=3). The same MIP receptor for both MISPE and the corresponding sensor was able to enhance the preconcentration of analyte substantially so as to attain the desired level of sensitivity; and that to without any interference (cross-reactivity) from other structurally related analogues including the major interferent like ascorbic acid prevalent in the aqueous environment of biological samples.
Prasad
Bhim Bali
BB
Analytical Division, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India. prasadbb_2000@yahoo.com
Sharma
Piyush Sindhu
PS
Lakshmi
Dhana
D
D006801:Humans; D054802:Molecular Imprinting; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D014527:Uric Acid / Q000032:analysis / Q000097:blood* / Q000737:chemistry
D011108:Polymers; D014527:Uric Acid; D058428:Silica Gel; D012822:Silicon Dioxide