Imaging Drosophila gene activation and polymerase pausing in vivo.
Since the early 1960s, imaging studies of Drosophila sp. polytene chromosomes have provided unique views of gene transcription in vivo. The dramatic changes in chromatin structure that accompany gene activation can be visualized as chromosome puffs. Now, live-cell imaging techniques coupled with protein-DNA crosslinking assays on a genome-wide scale allow more detailed mechanistic questions to be addressed and are prompting the re-evaluation of models of transcription regulation in both Drosophila and mammals.
Lis
John T
JT
Molecular Biology and Genetics, 416 Biotechnology Building, Cornell University, Ithaca, New York 14853, USA. jtl10@cornell.edu
D000818:Animals; D004330:Drosophila / Q000166:cytology / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D029721:Drosophila Proteins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012319:RNA Polymerase II / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics; D015533:Transcriptional Activation
D029721:Drosophila Proteins; D012319:RNA Polymerase II