title: Streptomyces radiopugnans sp. nov., a radiation-resistant actinomycete isolated from radiation-polluted soil in China.
abstract: The taxonomic position of an actinomycete isolated from radiation-polluted soil collected in Xinjiang Province, north-west China, was determined by using a polyphasic approach. The isolate, designated strain R97T, had chemical and morphological properties characteristic of streptomycetes. An almost-complete 16S rRNA gene sequence of the isolate was generated and compared with corresponding sequences of representative streptomycetes. The 16S rRNA data not only supported the classification of the strain in the genus Streptomyces but also showed that it represented a distinct phyletic line that was most closely, albeit loosely, associated with three other thermotolerant organisms, namely Streptomyces macrosporus NBRC 14748T, Streptomyces megasporus NBRC 14749T and Streptomyces thermolineatus NBRC 14750T. Strain R97T could be distinguished from these organisms based on a range of phenotypic properties. It is proposed that R97T (=CGMCC 4.3519T=DSM 41901T) be classified as the type strain of a novel species in the genus Streptomyces, Streptomyces radiopugnans sp. nov. The organism was shown to be resistant to 60Co gamma radiation at a dose of 15 kGy.
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang Uigur Autonomous Region, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Mao
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang Uigur Autonomous Region, PR China.
authors.forename: Qiyong
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang Uigur Autonomous Region, PR China.
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang Uigur Autonomous Region, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang Uigur Autonomous Region, PR China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Zhiheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang Uigur Autonomous Region, PR China.
authors.forename: Yuhu
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Division of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Goodfellow
mesh_terms: D015373:Bacterial Typing Techniques; D002681:China; D003037:Cobalt Radioisotopes; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005720:Gamma Rays; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011836:Radiation Tolerance; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012990:Soil Pollutants, Radioactive; D013045:Species Specificity; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology / Q000528:radiation effects*
chemical_list: D003037:Cobalt Radioisotopes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012990:Soil Pollutants, Radioactive