Determination of enantiomeric excess of ethyl 3,5-dihydroxy-6-benzyloxy hexanoate by chiral reverse phase high performance liquid chromatography.
A simple and reliable chiral HPLC method was developed for the determination of enantiomeric excess of a chiral dihydroxy intermediate for the chemoenzymatic synthesis of side chain of statin drugs. After evaluating different columns and conditions, the four stereoisomers of ethyl 3,5-dihydroxy-6-benzyloxy hexanoate were well resolved by a simple gradient elution on OD-RH column, and the enantiomeric excess of the desired 3R,5S-enantiomer was accurately measured. This study provides a simple, rapid, accurate, and reliable method to assess the enantiomeric quality of such important intermediates.
Liu
Ji-Hua
JH
School of Life Science and Technology, China Pharmaceutical University, Nanjing, People's Republic of China.
Yu
Bo-Yang
BY
Chen
Yi-Jun
YJ
D002208:Caproates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015203:Reproducibility of Results; D013237:Stereoisomerism
D002208:Caproates