title: Salmonella infection of afferent lymph dendritic cells.
abstract: The interactions of Salmonella enterica subspecies I serotype Abortusovis (S. Abortusovis) with ovine afferent lymph dendritic cells (ALDCs) were investigated for their ability to deliver Maedi visna virus (MVV) GAG p25 antigens to ALDCs purified from afferent lymph. Salmonellae were found to enter ALDC populations by a process of cell invasion, as confirmed by electron and confocal microscopy. This led to phenotypical changes in ALDC populations, as defined by CD1b and CD14 expression. No differences in the clearance kinetics of intracellular aroA-negative Salmonella from CD1b+ CD14lo and CD1b+ CD14(-) ALDC populations were noted over 72 h. ALDCs were also shown to present MVV GAG p25 expressed by aroA-negative S. Abortusovis to CD4+ T lymphocytes. Thus, the poor immune responses that Salmonella vaccines elicited in large animal models compared with mice are neither a result of an inability of Salmonella to infect large animal DCs nor an inability of these DCs to present delivered antigens. However, the low efficiency of infection of ALDC compared with macrophages or monocyte-derived DCs may account for the poor immune responses induced in large animal models.
authors.affiliation: Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 0ES, UK.
authors.forename: Simon S M
authors.initials: SS
authors.lastname: Chan
authors.forename: Pietro
authors.initials: P
authors.lastname: Mastroeni
authors.forename: Ian
authors.initials: I
authors.lastname: McConnell
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Blacklaws
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000818:Animals; D017951:Antigen Presentation; D015703:Antigens, CD / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure; D005260:Female; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000517:prevention & control; D011994:Recombinant Proteins / Q000276:immunology; D022562:Salmonella Vaccines / Q000276:immunology*; D019779:Salmonella enterica / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D012756:Sheep / Q000276:immunology*; D014436:Typhoid-Paratyphoid Vaccines / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*; D014790:Visna-maedi virus / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D015683:Gene Products, gag; D011994:Recombinant Proteins; D022562:Salmonella Vaccines; D014436:Typhoid-Paratyphoid Vaccines; D014765:Viral Vaccines; C087350:aroA Salmonella vaccine