Application of Diol column under adsorption and mixed partition-adsorption normal-phase liquid chromatography mode for the separation of purines and pyrimidines.
The diol-bonded phase (column LiChrospher 100 Diol) has been studied for the separation of some purines and pyrimidines under normal-phase liquid chromatography (NPLC) conditions. Retention time, column efficiency, and selectivity of column with diol-phase were compared to those of unmodified silica (column LiChrospher SI-60). It was established that under adsorption NPLC mode application of diol-phase can reduce the separation time and a little improve the column efficiency. Significant improvement of the column efficiency for polar solutes is observed if mixed partition-adsorption NPLC mode is used. The investigation has shown that application of diol-phase instead of bare silica is useful not only under adsorption, but also under mixed partition-adsorption mode if the system with specific selectivity is necessary.
Kazoka
H
H
Latvian Institute of Organic Synthesis, 21 Aizkraukles Street, LV-1006 Riga, Latvia. helena@osi.lv
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D011687:Purines / Q000737:chemistry / Q000302:isolation & purification*; D011743:Pyrimidines / Q000737:chemistry / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry
D011687:Purines; D011743:Pyrimidines; D012822:Silicon Dioxide