Mass spectrometric detection of urinary oligosaccharides as markers of Schistosoma mansoni infection.
Current diagnosis of schistosomiasis is still not ideal. In the present study we evaluated a targeted affinity approach using mAb 114-4D12, reactive with a unique Schistosoma mansoni-specific glycan epitope, combined with matrix-assisted laser-desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry. For nine of 11 urine samples (1ml) from Egyptian individuals with different intensities of infection, a characteristic MALDI-TOF mass spectrum was observed, representing a series of fuco-oligosaccharides that are produced by schistosome eggs. The identification of these small molecule markers may lead to a new egg-load-related assay for light infections in schistosomiasis.
Robijn
Marjolein L M
ML
Department of Parasitology, Center for Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands.
Planken
Joost
J
Kornelis
Dieuwke
D
Hokke
Cornelis H
CH
Deelder
André M
AM
D000818:Animals; D015415:Biomarkers / Q000652:urine; D004534:Egypt; D006801:Humans; D009844:Oligosaccharides / Q000652:urine*; D012550:Schistosoma mansoni / Q000737:chemistry*; D012552:Schistosomiasis / Q000175:diagnosis* / Q000469:parasitology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D015415:Biomarkers; D009844:Oligosaccharides