Functional characterization of Pneumocystis carinii brl1 by transspecies complementation analysis.
Pneumocystis jirovecii is a fungus which causes severe opportunistic infections in immunocompromised humans. The brl1 gene of P. carinii infecting rats was identified and characterized by using bioinformatics in conjunction with functional complementation in Saccharomyces cerevisiae and Schizosaccharomyces pombe. The ectopic expression of this gene rescues null alleles of essential nuclear membrane proteins of the Brr6/Brl1 family in both yeasts.
Lo Presti
Libera
L
Institute of Microbiology, Centre Hospitalier Universitaire Vaudois and University of Lausanne, 1011 Lausanne, Switzerland.
Cockell
Moira
M
Cerutti
Lorenzo
L
Simanis
Viesturs
V
Hauser
Philippe M
PM
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology; D015966:Gene Expression Regulation, Fungal; D005816:Genetic Complementation Test; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000502:physiology*; D045363:Pneumocystis carinii / Q000378:metabolism*; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000502:physiology*; D012568:Schizosaccharomyces / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
C435210:Brr6 protein, S cerevisiae; D008565:Membrane Proteins; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins