title: DNA repair deficiency and acetaldehyde-induced chromosomal alterations in CHO cells.
abstract: Induction of chromosomal aberrations (CAs) and sister chromatid exchanges (SCEs) by acetaldehyde (AA) was evaluated in parental and different DNA repair-deficient Chinese hamster ovary (CHO) cell lines to elucidate the mechanisms involved in the protection against AA-induced chromosome damage. Cell lines employed included the parental (AA8), nucleotide excision repair (UV4, UV5, UV61), base excision repair (EM9), homologous recombination repair (HRR) (irs1SF, 51D1)-deficient and Fanconi-like (KO40) ones. The ranking of different cell lines for sensitivity to induction of CAs by AA was 51D1 > irs1SF > KO40 > UV4 > V33-EM9-AA8 > UV61-UV5 in a descending order. Cells deficient in HRR were most sensitive followed by Fanconi anaemia like (KO40) suggesting these pathways, especially HRR is very important for the repair of AA-induced lesions. These observations also suggest that interstrand cross links are primary biologically relevant DNA lesions induced by AA for induction of CAs. Only marginal differences were found between the cell lines for induction of SCEs. The possible mechanisms involved in AA-induced chromosomal alterations are discussed.
authors.affiliation: Department of Agrobiology and Agrochemistry, University of Tuscia, Via S. C. De Lellis snc, I-01100 Viterbo, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Mechilli
authors.forename: Angelo
authors.initials: A
authors.lastname: Schinoppi
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kobos
authors.forename: Adayapalam T
authors.initials: AT
authors.lastname: Natarajan
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Palitti
mesh_terms: D000079:Acetaldehyde / Q000633:toxicity*; D000818:Animals; D016466:CHO Cells; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004260:DNA Repair; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D012854:Sister Chromatid Exchange
chemical_list: D009153:Mutagens; D000079:Acetaldehyde