Analysis of monosaccharides by capillary electrophoresis with electrochemiluminescence detection.
A capillary electrophoresis with tris(2,2'-bipyridyl)ruthenium(II)-based electrogenerated chemiluminescence detection method was investigated for the determination of monosaccharides using 2-diethylaminoethanethiol as a derivatizing reagent. Xylose, rhamnose, glucose and glucosamine were selected for the demonstration of the method. Under optimal conditions, the calibration curve of glucose was linear over the range of 1.0 x 10(-4) to 1.0 x 10(-7) mol/L (R = 0.999) and the detection limit was 6.0 x 10(-8) mol/L (S/N = 3). The method was successfully applied for the assay of glucose in angelica.
Li
Ming
M
Department of Chemistry, Pohang University of Science and Technology, Pohang, Korea.
Lee
Sang Hak
SH
D000097:Acetonitriles; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000379:methods*; D015394:Molecular Structure; D009005:Monosaccharides / Q000032:analysis* / Q000737:chemistry
D000097:Acetonitriles; D002021:Buffers; D009005:Monosaccharides; C032159:acetonitrile