title: Eucaryotic diversity in a hypersaline microbial mat.
abstract: To determine the eucaryotic diversity of the hypersaline Guerrero Negro microbial mat, we amplified 18S rRNA genes from DNA extracted from this mat and constructed and analyzed clone libraries. The extent of eucaryotic diversity detected was remarkably low, only 15 species among 890 clones analyzed. Six eucaryotic kingdoms were represented, as well as a novel cluster of sequences. Nematode sequences dominated the clone libraries.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 90309-0347, USA.
authors.forename: Leah M
authors.initials: LM
authors.lastname: Feazel
authors.forename: John R
authors.initials: JR
authors.lastname: Spear
authors.forename: Alicia B
authors.initials: AB
authors.lastname: Berger
authors.forename: J Kirk
authors.initials: JK
authors.lastname: Harris
authors.forename: Daniel N
authors.initials: DN
authors.lastname: Frank
authors.forename: Ruth E
authors.initials: RE
authors.lastname: Ley
authors.forename: Norman R
authors.initials: NR
authors.lastname: Pace
mesh_terms: D044822:Biodiversity; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005057:Eukaryotic Cells / Q000145:classification*; D019015:Geologic Sediments; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal