title: [Diagnostic value of p16INK4a in squamous intraepithelial lesion in gynecologic cytology].
abstract: OBJECTIVE
  To study the diagnostic value of p16(INK4a) in squamous intraepithelial lesion in gynecologic cytology and its relationship with types of human papillomavirus (HPV).
  
  
  METHODS
  88 liquid-based gynecologic cytology cases with histologic correlation, including 20 cases of cervicitis, 18 cases of low-grade squamous intraepithelial lesion (LSIL), 34 cases of high-grade squamous intraepithelial lesion (HSIL) and 16 cases of squamous cell carcinoma (SCC), were enrolled into the study. Immunocytochemistry for p16(INK4a) protein and polymerase chain reaction-based HPV DNA testing (for HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66 and 68, as well as HPV types 6, 11, 42, 43 and 44) were performed.
  
  
  RESULTS
  The rate of expression of p16(INK4a) protein was 0, 27.8%, 100% and 100% in the cervicitis group, LSIL group, HSIL group and SCC group, respectively. The expression was significantly higher in the latter 3 groups than that in the cervicitis group (P < 0.01). Besides, the expression was significantly higher in cases associated with high-risk HPV genotypes (96.4%) than in cases associated with low-risk HPV genotypes (7.7%).
  
  
  CONCLUSIONS
  p16(INK4a) is a valuable biomarker for detection of HPV-related dysplastic squamous cells, with high sensitivity and specificity.
authors.affiliation: Department of Pathology, Guangdong Provincial People's Hospital, Guangzhou 510080, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Mei
authors.forename: Yan-hui
authors.initials: YH
authors.lastname: Liu
authors.forename: Heng-guo
authors.initials: HG
authors.lastname: Zhuang
authors.forename: Xin-lan
authors.initials: XL
authors.lastname: Luo
authors.forename: Ke-lin
authors.initials: KL
authors.lastname: Xiao
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000378:metabolism / Q000821:virology; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D003937:Diagnosis, Differential; D005260:Female; D005838:Genotype; D006801:Humans; D007150:Immunohistochemistry; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000378:metabolism / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000821:virology; D002575:Uterine Cervicitis / Q000175:diagnosis / Q000378:metabolism / Q000821:virology
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16