[Influence of artificial pneumoperitoneal media on colon carcinoma cell proliferation in vitro].
OBJECTIVE
To evaluate the influence of different pneumoperitoneal media on colon carcinoma LS-174T cell proliferation in vitro.


METHODS
The artificial pneumoperitoneum was established. The proliferation of LS-174T cells was detected by MTT assay and soft agar clone formation assay. Expression of HIF-1alpha and VEGF was examined by immunohistochemistry. Apoptosis of LS-174T cells was analyzed by AO/EB double fluorescein stain and flow cytometry.


RESULTS
The growth speed and proliferating capacity of LS-174T cells in CO(2) pneumoperitoneum group[A:0.37 +/- 0.02,formation (32.8 +/- 3.6)%] were significantly higher than those in control group [A:0.33 +/- 0.01,formation (28.4 +/- 2.3)%] and He group [A:0.30 +/- 0.01,formation (23.5 +/- 2.7)%], meanwhile the He group was the lowest (P<0.01). Positive expression of HIF-1alpha and VEGF in CO(2) and He artificial pneumoperitoneum up-regulated significantly as compared to control group(P<0.01), meanwhile the above expression was higher in CO(2) group (P<0.01). The G(0 )/G(1) ratio in CO(2) group was the lowest as compared to control group and He group (P<0.01), and G(0 )/G(1) ratio in He group was higher than that of control group(P<0.01). Aapoptosis rate in He group was the highest as compared with the other two groups(P<0.01).


CONCLUSIONS
CO(2) pneumoperitoneum has stronger effect on the proliferation of colon carcinoma cell LS-174T as compared to He pneumoperitoneum in vitro.
Tian
Yong-Gang
YG
Department of ICU, The Second Affiliated Hospital, Harbin Medical University, Harbin, China.
Tang
Yin-Hua
YH
Xu
Jun
J
Dai
Wen-Jie
WJ
Liu
Su
S
Zhao
Jin-Peng
JP
Ma
Yong
Y
Jiang
Hong-Chi
HC
D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D005434:Flow Cytometry; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D011028:Pneumoperitoneum, Artificial / Q000379:methods*
D051795:Hypoxia-Inducible Factor 1, alpha Subunit