Gene expression profiling related to the enhanced erythropoiesis in mouse bone marrow cells.
Peroxiredoxin II knockout (Prdx II(-/-)) mice had a spontaneous phenotype of hemolytic anemia. In this study, we found that Ter-119(+)CD71(+) cells increased in Prdx II(-/-) mice bone marrow (BM) at 8 weeks of age. We examined the differential expression profiles to bone marrow cells (BMCs) between Prdx II(+/+) and Prdx II(-/-) mice using a cDNA microarray. We identified the 136 candidates were differentially expressed a greater twofold increase or decrease than EPO receptor. In this study, we focused on the up-regulated NBPs during erythropoietic differentiation. According to cDNA microarray results, six NBPs except zfp-127 were up-regulated during erythropoiesis in Prdx II(-/-) mice. Among the six candidates, eIF3-p44, hnRNPH1, G3bp, and Zfpm-1 were dramatically increased at day 7 of the in vitro erythropoietic differentiation of human CD34(+) cells. However, DJ-1 and Rbm3 were slightly increased only at day 12. Our results suggest that up-regulated NBPs might be involved during erythropoietic differentiation.
Yang
Hee-Young
HY
Department of Molecular Medicine, Chonnam National University, Gwangju, South Korea.
Jeong
Dong Kee
DK
Kim
Seok-Ho
SH
Chung
Kyoung-Jin
KJ
Cho
Eun-Jin
EJ
Jin
Cheng Hao
CH
Yang
Ung
U
Lee
Sang Ryeul
SR
Lee
Dong-Seok
DS
Lee
Tae-Hoon
TH
D000743:Anemia, Hemolytic / Q000235:genetics; D000818:Animals; D018952:Antigens, CD34; D001854:Bone Marrow Cells / Q000502:physiology; D004920:Erythropoiesis / Q000235:genetics*; D020869:Gene Expression Profiling; D006412:Hematopoietic Stem Cells; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D054464:Peroxiredoxins / Q000235:genetics; D017467:Receptors, Erythropoietin; D015854:Up-Regulation
D018952:Antigens, CD34; D017467:Receptors, Erythropoietin; C086065:PRDX2 protein, human; D054464:Peroxiredoxins