title: Study of a monolithic silica capillary column coated with poly(octadecyl methacrylate) for the reversed-phase liquid chromatographic separation of some polar and non-polar compounds.
abstract: The performance of a monolithic silica capillary column coated with poly(octadecyl methacrylate) (ODM column) for the reversed-phase liquid chromatographic separation of some polar and non-polar compounds was studied, and the results were compared to those obtained by using a monolithic silica capillary column modified with octadecylsilyl-(N,N-diethylamino)silane (ODS column). Benzene and naphthalene derivatives, polycyclic aromatic hydrocarbons (PAHs), steroids, alkyl phthalates, and tocopherol homologues were used as test samples. In general, compounds with aromatic character, rigid and planar structures, and lower length-to-breadth ratios (more compacted structures) seem to have more preference for the polymer coated stationary phase (ODM). Compounds with acidic character have also a higher retention on ODM columns because of the presence of ester groups in the stationary phase. The polymer coated column allowed the separation of some PAHs, alkyl phthalates, steroids, and of beta- and gamma-tocopherol isomers which cannot be separated under the same conditions on ODS columns, while keeping similar column efficiency. These results allowed to suggest ODM columns as a good alternative to conventional ODS columns for reversed-phase liquid chromatography.
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology, Goisho-Kaido-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. oscarnubu@yahoo.es
authors.forename: Oscar
authors.initials: O
authors.lastname: Núñez
authors.forename: Tohru
authors.initials: T
authors.lastname: Ikegami
authors.forename: Kosuke
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Nobuo
authors.initials: N
authors.lastname: Tanaka
mesh_terms: D001555:Benzene Derivatives / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000379:methods*; D004785:Environmental Pollutants / Q000302:isolation & purification; D009281:Naphthalenes / Q000302:isolation & purification; D009930:Organic Chemicals / Q000737:chemistry / Q000302:isolation & purification*; D010795:Phthalic Acids / Q000302:isolation & purification; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000302:isolation & purification; D011109:Polymethacrylic Acids / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013256:Steroids / Q000302:isolation & purification; D024505:Tocopherols / Q000737:chemistry / Q000302:isolation & purification; D014992:Xylenes / Q000302:isolation & purification
chemical_list: D001555:Benzene Derivatives; D004785:Environmental Pollutants; D009281:Naphthalenes; D009930:Organic Chemicals; D010795:Phthalic Acids; D011084:Polycyclic Aromatic Hydrocarbons; D011109:Polymethacrylic Acids; D013256:Steroids; D014992:Xylenes; C527028:poly(octadecyl methacrylate); C031721:naphthalene; D012822:Silicon Dioxide; C016834:3,5-xylenol; D024505:Tocopherols