Production of isopropanol by metabolically engineered Escherichia coli.
A genetically engineered strain of Escherichia coli JM109 harboring the isopropanol-producing pathway consisting of five genes encoding four enzymes, thiolase, coenzyme A (CoA) transferase, acetoacetate decarboxylase from Clostridium acetobutylicum ATCC 824, and primary-secondary alcohol dehydrogenase from C. beijerinckii NRRL B593, produced up to 227 mM of isopropanol from glucose under aerobic fed-batch culture conditions. Acetate production by the engineered strain was approximately one sixth that produced by a control E. coli strain bearing an expression vector without the clostridial genes. These results demonstrate a functional isopropanol-producing pathway in E. coli and consequently carbon flux from acetyl-CoA directed to isopropanol instead of acetate. This is the first report on isopropanol production by genetically engineered microorganism under aerobic culture conditions.
Jojima
Toru
T
Research Institute of Innovative Technology for the Earth , Kyoto, Japan.
Inui
Masayuki
M
Yukawa
Hideaki
H
D019840:2-Propanol / Q000032:analysis / Q000378:metabolism*; D019342:Acetic Acid / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D019149:Bioreactors; D053898:Biosynthetic Pathways; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D015256:Coenzyme A-Transferases / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D007218:Industrial Microbiology
D001426:Bacterial Proteins; D000426:Alcohol Dehydrogenase; D015256:Coenzyme A-Transferases; D002262:Carboxy-Lyases; C023743:acetoacetate decarboxylase; D019840:2-Propanol; D019342:Acetic Acid