Functional analysis of phenolsulfonphthalein transport system in Long-Evans Cinnamon rats.
It has been reported that the transport function for organic anions on the kidney is maintained in multidrug resistance-associated protein 2 (Mrp2)-deficient rats. Different from Mrp2-deficient rats, Long-Evans Cinnamon (LEC) rats have impaired urinary excretion of Mrp2-substrate, phenolsulfonphthalein (PSP). PSP is transported by the potential-sensitive urate transport system in rat brush-border membranes. We analyzed the function of PSP transport system in LEC rats. Unlike Long-Evans Agouti (LEA) rats, the initial uptake of PSP and urate into the renal brush-border membrane vesicles of LEC rats were not significantly enhanced in the presence of positive intravesicular potential, suggesting that the potential-sensitive urate transport system is impaired in LEC rats. LEC rats should be useful for elucidating the potential-sensitive urate transport system in rats at the molecular level.
Itagaki
Shirou
S
Laboratory of Clinical Pharmaceutics and Therapeutics, Division of Pharmasciences, Faculty of Pharmaceutical Sciences, Hokkaido University, and Department of Pharmacy, Hokkaido University Hospital, Sapporo, Japan.
Chiba
Makoto
M
Kobayashi
Masaki
M
Sugawara
Mitsuru
M
Kobayashi
Michiya
M
Hirano
Takeshi
T
Iseki
Ken
K
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008871:Microvilli / Q000187:drug effects; D010637:Phenolsulfonphthalein / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D020332:Rats, Inbred LEC; D053835:Unilamellar Liposomes / Q000378:metabolism; D014527:Uric Acid / Q000378:metabolism; D010130:p-Aminohippuric Acid / Q000378:metabolism
D053835:Unilamellar Liposomes; D014527:Uric Acid; D010637:Phenolsulfonphthalein; D010130:p-Aminohippuric Acid