[Nimesulide spectrophotometric determination in the visible region].
Nimesulide is a nonsteroidal antiinflammatory (NSAID) drug whose mechanism of action is characterized by selective inhibition of cyclooxygenase-2. Nimesulide have analgesic and antipyretic properties.


METHODS
For nimesulide analysis we developed a spectrophotometric method based on reduction of the nitro group of nimesulide by zinc and hydrochloric acid followed by diazotization and coupling with thymol (5-methyl-2-(1-methylethyl) phenol) in ammonia medium to form a stable chromophore which absorbs at 476 nm. There were established the practical working conditions. The developed method was validated.


RESULTS
The method showed a good linearity in the range of 5 - 40 microg / sample (the correlation coefficient r = 0.9990). The detection limit (LD) was 0.99 microg/sample and the quantification limit (LQ) was 3.32 microg/sample. There were established the system precision (RSD = 1.70%), the precision (RSD < 2.2%) and the accuracy (recovery in the range 97.66 - 102.69% with a mean recovery of 99.81%) of the method.


CONCLUSIONS
A new method for nimesulide spectrophotometric quantification in visible has been developed.
Perju
Andreia Claudia
AC
Facultatea de Farmacie, Disciplina de Chimie analitică, Universitatea de Medicină i Farmacie "Gr.T. Popa" Iaşi.
Mândrescu
Mariana
M
Spac
A F
AF
Dorneanu
V
V
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000493:pharmacokinetics; D016861:Cyclooxygenase Inhibitors / Q000032:analysis* / Q000493:pharmacokinetics; D006801:Humans; D013053:Spectrophotometry / Q000379:methods*; D013449:Sulfonamides / Q000032:analysis* / Q000493:pharmacokinetics
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016861:Cyclooxygenase Inhibitors; D013449:Sulfonamides; C012655:nimesulide