Expression and characterization of the integrase of bovine immunodeficiency virus.
In the pol gene of bovine immunodeficiency virus (BIV) there is a sequence, located between the reverse-transcriptase and integrase (IN)-encoding sequences, that is not found in HIV-1 pol gene and encodes a 74-residue polypeptide with homology to dUTPases. We have expressed two BIV IN versions that differ in their amino termini. The longer version, containing the 74-residue sequence, did not show any detectable 3'-end processing and strand transfer IN activities and performed only the IN-associated disintegration. Consequently, the shorter version, lacking the dUTPase-related residues, performed all three activities and is most likely similar to the viral enzyme. A comparison between BIV IN and the well-studied HIV-1 IN, with substrates that mimic the U5 LTR sequences of BIV, HIV-1 and another bovine lentivirus, Jembrana disease virus, revealed that the extra 3'-end sequence beyond the conserved "CA" is probably less important for IN activities than the sequence upstream to the "CA".
Avidan
Orna
O
Department of Cell and Developmental Biology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, 69978, Israel.
Hizi
Amnon
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D015684:Gene Products, pol / Q000737:chemistry / Q000235:genetics; D016325:HIV Long Terminal Repeat / Q000235:genetics; D015497:HIV-1 / Q000201:enzymology; D006801:Humans; D016087:Immunodeficiency Virus, Bovine / Q000201:enzymology* / Q000235:genetics; D019426:Integrases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids; D011755:Pyrophosphatases / Q000378:metabolism; D013379:Substrate Specificity; D020079:Terminal Repeat Sequences / Q000235:genetics
D015684:Gene Products, pol; D019426:Integrases; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase