Separation of plant pathogens from different hosts and tissues by capillary electromigration techniques.
In this contribution capillary isoelectric focusing and capillary zone electrophoresis were applied for the separation and detection of different plant pathogens including Pseudomonas syringae pv. syringae, P. syringae pv. lachrymans, Pseudomonas savastanoi pv. fraxinus, P. savastanoi pv. olea, Agrobacterium tumefaciens, A vitis, Xanthomonas arboricola pv. juglandis, X. campestris pv. zinniae, and Curtobacterium sp.. The UV detection and sensitive fluorescence detection of the native phytopathogens or those dynamically modified by the nonionogenic fluorescent tenside based on pyrenebutanoate were used. The isoelectric points of the labeled phytopathogens were found comparable with the pI of the native compounds. No influence of the hosts on pIs of the strains of the genus Pseudomonas was observed. The identification of plant pathogens by gas chromatographic analysis of fatty acid methyl esters was compared with results of capillary isoelectric focusing. Capillary electromigration was successfully applied for the separation of microbes directly from plant tissue suspensions.
Horká
Marie
M
Institute of Analytical Chemistry, v. v. i., Academy of Sciences of the Czech Republic, Veverí 97, 602 00 Brno, Czech Republic. horka@iach.cz
Horký
Jaroslav
J
Matousková
Hana
H
Slais
Karel
K
D016960:Agrobacterium tumefaciens; D001419:Bacteria / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D059828:Plant Cells; D010935:Plant Diseases / Q000382:microbiology*; D010944:Plants / Q000382:microbiology; D011549:Pseudomonas; D014974:Xanthomonas