Inhibitors of osteoclast differentiation from Cephalotaxus koreana.
Three new flavonoid glycosides ( 1- 3), 11-hydroxyhainanolidol ( 4), and a new dibenzylbutyrolactone lignan glycoside ( 5) were isolated from the aerial parts of Cephalotaxus koreana Nakai, along with 19 known flavonoids. The structures of the new compounds were elucidated using spectroscopic evidence, primarily NMR and MS. Twenty-four compounds were isolated, and among these isoscutellarein 5-O-beta-D-glucopyranoside ( 3), apigenin ( 6), kaempferol 3-O-alpha-L-rhamnopyranosyl(1'''-->6'')-beta-D-glucopyranoside ( 7), tamarixetin 3-O-alpha-L-rhamnopyranosyl(1'''-->6'')-beta-D-glucopyranoside ( 8), quercetin 3-O-[6''-O-acetyl]-beta-D-glucopyranoside ( 9), and quercetin 3-O-alpha-L-rhamnopyranoside ( 10) showed significant inhibitory activities against osteoclast differentiation at concentrations of 0.1 and 1.0 microg/mL.
Yoon
Kee Dong
KD
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea.
Jeong
Doc Gyun
DG
Hwang
Yun Ha
YH
Ryu
Jei Man
JM
Kim
Jinwoong
J
D002454:Cell Differentiation / Q000187:drug effects*; D032483:Cephalotaxus / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006027:Glycosides; D007723:Korea; D015394:Molecular Structure; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry*
C527021:6-methylaromadendrin 3-O-beta-glucopyranoside; D005419:Flavonoids; D005960:Glucosides; D006027:Glycosides; C527023:apigenin 5-O-rhamnopyranosyl(1''''2'')(6''-O-acetyl)glucopyranoside; C527022:isoscutellarein 5-O-beta-glucopyranoside