title: Cell microarray for functional exploration of genomes.
abstract: As more genomes are sequenced, challenge of rapidly unraveling the functions of genes was faced. To that end, cell microarrays have been recently described that permit transfection of thousands of nucleic acids in parallel and enable the analysis of phenotypic consequences of such perturbations. As many parameters can influence the efficiency of transfection and consequently protein expression or extinction, some important features in manufacturing cell microarrays for functional exploration of genomes were described.
authors.affiliation: CEA, DSV, DRR, Functional Genomics Department, Evry, France.
authors.forename: David
authors.initials: D
authors.lastname: Castel
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Debily
authors.forename: Amandine
authors.initials: A
authors.lastname: Pitaval
authors.forename: Xavier
authors.initials: X
authors.lastname: Gidrol
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D020868:Gene Silencing; D016678:Genome; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D009696:Nucleic Acids / Q000378:metabolism; D046888:Tissue Array Analysis / Q000379:methods*; D014162:Transfection
chemical_list: D009696:Nucleic Acids; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins