title: Production of surfactant and detergent-stable, halophilic, and alkalitolerant alpha-amylase by a moderately halophilic Bacillus sp. Strain TSCVKK.
abstract: A moderately halophilic alkalitolerant Bacillus sp. Strain TSCVKK, with an ability to produce extracellular halophilic, alkalitolerant, surfactant, and detergent-stable alpha-amylase was isolated from soil samples obtained from a salt-manufacturing industry in Chennai. The culture conditions for higher amylase production were optimized with respect to NaCl, substrate, pH, and temperature. Maximum amylase production of 592 mU/ml was achieved in the medium at 48 h with 10% NaCl, 1% dextrin, 0.4% yeast extract, 0.2% tryptone, and 0.2% CaCl(2) at pH 8.0 at 30 degrees C. The enzyme activity in the culture supernatant was highest with 10% NaCl at pH 7.5 and 55 degrees C. The amylase that was partially purified by acetone precipitation was highly stable in various surfactants and detergents. Glucose, maltose, and maltooligosaccharides were the main end products of starch hydrolysis indicating that it is an alpha-amylase.
authors.affiliation: Biochemistry Laboratory, Department of Chemistry, Indian Institute of Technology Madras, Chennai, 600 036, India.
authors.forename: Kondepudi Kanthi
authors.initials: KK
authors.lastname: Kiran
authors.forename: T S
authors.initials: TS
authors.lastname: Chandra
mesh_terms: D000096:Acetone / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D005591:Chemical Fractionation; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003902:Detergents / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007194:India; D008320:Maltose / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013213:Starch / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors; D000516:alpha-Amylases / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D003902:Detergents; D004791:Enzyme Inhibitors; D009844:Oligosaccharides; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013501:Surface-Active Agents; C021705:maltooligosaccharides; D000096:Acetone; D008320:Maltose; D013213:Starch; D000516:alpha-Amylases; D005947:Glucose