[Effects of volatie oil of Schizonepeta tenuifolia Briq herb and Saposhnikovia divaricata Schischke root on proinflammatory cytokine expression and regulation].
OBJECTIVE
To study the effects of volatie oil of Schizonepeta tenuifolia Briq herb and Saposhnikovia divaricata Schischke root (OSS) on proinflammatory cytokine expression and regulation in rats.


METHODS
OA and LPS were injected intravenously to rats to develop acute lung injury (ALI). The rats were treated with OSS (45.19 microL kg(-1)). The pathological sections of lung tissue were prepared and observed in acute lung injury rats. The expression of nuclear factor-kappa B p65 (NF-kappaB p65), intercellar adhesion molecule CD54, and NF-kappaB p65 mRNA were determined in lung cells.


RESULTS
volatie oil of Schizonepeta tenuifolia Briq herb and Saposhnikovia divaricata Schischke root significantly inhibited the expression of CD54, the activation of NF-kappaB p65, and the transcription of NF-kappaB p65 mRNA.


CONCLUSIONS
OSS can reduce the expression of CD54 and NF-kappaB p65 protein synthesis, which may be its anti-inflammatory molecular mechanisms.
Ge
Wei-hong
WH
Zhejiang Chinese Medical University, Hangzhou 310053, China. geweihong@sina.com
Guo
Jian-you
JY
Shen
Ying-jun
YJ
Chen
Min-li
ML
Shi
Sen-lin
SL
Han
Yu-huan
YH
Lin
Jie
J
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology; D019661:Apiaceae / Q000737:chemistry; D004338:Drug Combinations; D005786:Gene Expression Regulation / Q000187:drug effects; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D019686:Lamiaceae / Q000737:chemistry; D008070:Lipopolysaccharides; D008297:Male; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D019301:Oleic Acid; D010938:Plant Oils / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D051996:Transcription Factor RelA / Q000096:biosynthesis* / Q000235:genetics
D000893:Anti-Inflammatory Agents; D004338:Drug Combinations; D008070:Lipopolysaccharides; D009822:Oils, Volatile; D010938:Plant Oils; D012333:RNA, Messenger; D051996:Transcription Factor RelA; D018799:Intercellular Adhesion Molecule-1; D019301:Oleic Acid