Generation of a Mig-6 conditional null allele.
Mitogen-inducible gene 6 (Mig-6) is a stress-induced gene that serves as a negative regulator of epidermal growth factor (EGF) signaling and acts as a tumor suppressor. Ablation of Mig-6 results in a significant percentage of embryo lethality as well as abnormalities in multiple tissues. To understand the physiological roles of Mig-6, a conditional null allele, Mig-6(f/f) was generated by introducing LoxP sites that flank exons 2 and 4. The Mig-6(f/f) allele was validated by generating recombined Mig-6(-/-) mice using the Zp3-Cre system. The conditional null allele was confirmed by assaying for Mig-6 gene expression in liver, lung, uterus, and skin. The recombined Mig-6(-/-) mice developed pathological changes, such as degenerative joint diseases and skin hyperplasia similar to the previously reported Mig-6 germline null allele. In addition, these mice also had enlarged uteri with endometrial hyperplasia. In summary, this Mig-6(f/f) mouse is a useful tool for the functional study of the Mig-6 gene in a tissue-specific fashion.
Jin
Nili
N
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
Gilbert
Jennifer L
JL
Broaddus
Russell R
RR
Demayo
Francesco J
FJ
Jeong
Jae-Wook
JW
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism*; D000483:Alleles; D000818:Animals; D048430:Cell Shape; D005818:Genetic Engineering / Q000379:methods*; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D018345:Mice, Knockout; D012867:Skin / Q000166:cytology / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; C475819:Errfi1 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins