Bicaudal-C recruits CCR4-NOT deadenylase to target mRNAs and regulates oogenesis, cytoskeletal organization, and its own expression.
Bicaudal-C (Bic-C) encodes an RNA-binding protein required maternally for patterning the Drosophila embryo. We identified a set of mRNAs that associate with Bic-C in ovarian ribonucleoprotein complexes. These mRNAs are enriched for mRNAs that function in oogenesis and in cytoskeletal regulation, and include Bic-C RNA itself. Bic-C binds specific segments of the Bic-C 5' untranslated region and negatively regulates its own expression by binding directly to NOT3/5, a component of the CCR4 core deadenylase complex, thereby promoting deadenylation. Bic-C overexpression induces premature cytoplasmic-streaming, a posterior-group phenotype, defects in Oskar and Kinesin heavy chain:betaGal localization as well as dorsal-appendage defects. These phenotypes are largely reciprocal to those of Bic-C mutants, and they affect cellular processes that Bic-C-associated mRNAs are known, or predicted, to regulate. We conclude that Bic-C regulates expression of specific germline mRNAs by controlling their poly(A)-tail length.
Chicoine
Jarred
J
Department of Biology and Developmental Biology Research Initiative, McGill University, 1205 Avenue Docteur Penfield, Montréal, Québec H3A 1B1, Canada.
Benoit
Perrine
P
mRNA Regulation and Development, Institut de Génétique Humaine, CNRS, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France.
Gamberi
Chiara
C
Department of Biology and Developmental Biology Research Initiative, McGill University, 1205 Avenue Docteur Penfield, Montréal, Québec H3A 1B1, Canada.
Paliouras
Miltiadis
M
Department of Biology and Developmental Biology Research Initiative, McGill University, 1205 Avenue Docteur Penfield, Montréal, Québec H3A 1B1, Canada.
Simonelig
Martine
M
mRNA Regulation and Development, Institut de Génétique Humaine, CNRS, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France.
Lasko
Paul
P
Department of Biology and Developmental Biology Research Initiative, McGill University, 1205 Avenue Docteur Penfield, Montréal, Québec H3A 1B1, Canada. Electronic address: paul.lasko@mcgill.ca.
D020121:5' Untranslated Regions; D000818:Animals; D001693:Biological Transport, Active; D019521:Body Patterning; D003593:Cytoplasm / Q000378:metabolism; D003599:Cytoskeleton / Q000502:physiology*; D004330:Drosophila / Q000196:embryology / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D005260:Female; D009154:Mutation; D009866:Oogenesis; D011061:Poly A / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000502:physiology*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D012260:Ribonucleases / Q000235:genetics / Q000502:physiology*
D020121:5' Untranslated Regions; C113767:BicC protein, Drosophila; D029721:Drosophila Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011061:Poly A; C501437:CCR4 protein, Drosophila; D012260:Ribonucleases