title: Differential in vivo potential of endothelial progenitor cells from human umbilical cord blood and adult peripheral blood to form functional long-lasting vessels.
abstract: Tissue engineering requires formation of a de novo stable vascular network. Because of their ability to proliferate, differentiate into endothelial cells, and form new vessels, blood-derived endothelial progenitor cells (EPCs) are attractive source of cells for use in engineering blood vessels. However, the durability and function of EPC-derived vessels implanted in vivo are unclear. To this end, we directly compared formation and functions of tissue-engineered blood vessels generated by peripheral blood- and umbilical cord blood-derived EPCs in a model of in vivo vasculogenesis. We found that adult peripheral blood EPCs form blood vessels that are unstable and regress within 3 weeks. In contrast, umbilical cord blood EPCs form normal-functioning blood vessels that last for more than 4 months. These vessels exhibit normal blood flow, perm-selectivity to macromolecules, and induction of leukocyte-endothelial interactions in response to cytokine activation similar to normal vessels. Thus, umbilical cord blood EPCs hold great therapeutic potential, and their use should be pursued for vascular engineering.
authors.affiliation: Edwin L. Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
authors.forename: Patrick
authors.initials: P
authors.lastname: Au
authors.forename: Laurence M
authors.initials: LM
authors.lastname: Daheron
authors.forename: Dan G
authors.initials: DG
authors.lastname: Duda
authors.forename: Kenneth S
authors.initials: KS
authors.lastname: Cohen
authors.forename: James A
authors.initials: JA
authors.lastname: Tyrrell
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Lanning
authors.forename: Dai
authors.initials: D
authors.lastname: Fukumura
authors.forename: David T
authors.initials: DT
authors.lastname: Scadden
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Jain
mesh_terms: D000328:Adult; D001808:Blood Vessels / Q000166:cytology*; D002460:Cell Line; D002463:Cell Membrane Permeability; D049109:Cell Proliferation; D002469:Cell Separation; D042783:Endothelial Cells / Q000166:cytology*; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D013997:Time Factors