title: Canine model of ischemic stroke with permanent middle cerebral artery occlusion: clinical and histopathological findings.
abstract: The aim of the present study was to assess the clinical and histopathological findings in a canine model of ischemic stroke. Cerebral ischemic stroke was induced by middle cerebral artery occlusion in four healthy beagle dogs using silicone plugs. They showed neurological signs of forebrain dysfunction such as reduced responsiveness, head turning, circling, postural reaction deficits, perceptual deficits, and hemianopsia. These signs gradually regressed within 4 weeks without therapy. On magnetic resonance imaging, T2 hyperintensity and T1 hypointensity were found in the cerebral cortex and basal ganglia. These lesions were well-defined and sharply demarcated from adjacent brain parenchyma with a homogenous appearance. No abnormalities of the cerebrospinal fluid were observed. At necropsy, atrophic and necrotic lesions were observed in the cerebral cortex. The cerebral cortex, basal ganglia, and thalamus were partially unstained with triphenyl- tetrazolium chloride. Histopathologically, typical features of infarction were identified in cortical and thalamic lesions. This study demonstrates that our canine model resembles the conditions of real stroke patients.
authors.affiliation: Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea.
authors.forename: Byeong-Teck
authors.initials: BT
authors.lastname: Kang
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Lee
authors.forename: Dong-In
authors.initials: DI
authors.lastname: Jung
authors.forename: Chul
authors.initials: C
authors.lastname: Park
authors.forename: Su-Hyun
authors.initials: SH
authors.lastname: Gu
authors.forename: Hyo-Won
authors.initials: HW
authors.lastname: Jeon
authors.forename: Dong-Pyo
authors.initials: DP
authors.lastname: Jang
authors.forename: Chae-Young
authors.initials: CY
authors.lastname: Lim
authors.forename: Fu-Shi
authors.initials: FS
authors.lastname: Quan
authors.forename: Young-Bo
authors.initials: YB
authors.lastname: Kim
authors.forename: Zang-Hee
authors.initials: ZH
authors.lastname: Cho
authors.forename: Eung-Je
authors.initials: EJ
authors.lastname: Woo
authors.forename: Hee-Myung
authors.initials: HM
authors.lastname: Park
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002544:Cerebral Infarction / Q000209:etiology* / Q000473:pathology*; D002555:Cerebrospinal Fluid / Q000737:chemistry / Q000166:cytology; D004195:Disease Models, Animal; D004285:Dogs; D020244:Infarction, Middle Cerebral Artery / Q000150:complications* / Q000473:pathology*; D008279:Magnetic Resonance Imaging; D008297:Male