[Exploration on relationship between TCM syndrome type and expression of human leucocyte antigen locus-DRB1 in patients with chronic hepatitis B].
OBJECTIVE
To study the relationship between TCM syndrome type and expression of human leucocyte antigen-DRB1 (HLA-DRB1) in patients with chronic hepatitis B.


METHODS
Using PCR method to amplify the related segments of DNA extracted from peripheral leucocytes by the routine methods, and gene array analysis was performed to detect the expression of HLA-DRB1.


RESULTS
HLA-DRB1 * 13 was expressed in healthy person in the control group, but was not expressed in chronic hepatitis B patients, showing significant difference between the two groups. In the patients with five different syndrome types, i.e. the dampness blocking middle-jiao type (A), the Gan-stagnancy with Pi-deficiency type (B), the blood stasis blocking collaterals type (C), the Gan-Shen yin-deficiency type (D) and the Pi-Shen yang-deficiency type (E), the former three belonged to the excessive syndrome and the latter two were deficient syndrome. Most of the CHB patients were differentiated as excessive syndrome.


CONCLUSIONS
Difference of HLA-DR expression exists between chronic hepatitis B patients and healthy persons, the action of the difference is remained for further confirmation. HLA-DR expression in patients with different syndrome types, excessive or deficient, might be different, too.
Wang
Rong-bing
RB
Department of Integrative Traditional Chinese and Western Medicine, Ditan Hospital, Beijing. n-y@tom.com
Zhou
Gui-qin
GQ
Jiang
Yu-yong
YY
D000328:Adult; D000483:Alleles; D003937:Diagnosis, Differential; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D019694:Hepatitis B, Chronic / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008297:Male; D008516:Medicine, Chinese Traditional; D013577:Syndrome; D055815:Young Adult
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains