Spectrophotometric method for quantitative determination of total anthocyanins and quality characteristics of roselle (Hibiscus sabdariffa).
A simple, rapid and cost-saving method for the determination of total anthocyanins in roselle has been developed. The method was based on pH-differential spectrophotometry. The calibration curve of the major anthocyanin in roselle, delphinidin 3-sambubioside (Dp-3-sam), was constructed by using methyl orange and their correlation factor. The reliability of this developed method was comparable to the direct method using standard Dp-3-sam and the HPLC method. Quality characteristics of roselle produced in Thailand were also reported. Its physical quality met the required specifications. The overall chemical quality was herein surveyed for the first time and it was found to be the important parameter corresponded to the commercial grading of roselle. Total contents of anthocyanins and phenolics were proportional to the antiradical capacity.
Sukwattanasinit
Tasamaporn
T
Faculty of Pharmacy, Silpakorn University, Nakhon-pathom, Thailand.
Burana-Osot
Jankana
J
Sotanaphun
Uthai
U
D000872:Anthocyanins / Q000737:chemistry*; D031584:Hibiscus / Q000737:chemistry*; D013053:Spectrophotometry
D000872:Anthocyanins