title: Clinical alterations and mRNA levels of IL-4 and IL-5 in bronchoalveolar cells of horses with transient pulmonary eosinophilia.
abstract: The aim of this study was to assess clinical signs and altered pulmonary cell expression of cytokines related to eosinophil kinetics in horses with pulmonary eosinophilia. Pulmonary eosinophilia was detected by bronchoalveolar lavage (BAL) in a group of standardbreds in training. Horses had detailed clinical examination, bronchoscopy, endobronchial biopsy and BAL on three occasions at approximately 6 month intervals. During the second sampling period BAL eosinophils were significantly elevated (p>0.010), with five horses having from 5% to 37% eosinophils in BAL. Neither detailed clinical examination parameters nor gene expression of IL-4 and IL-5 mRNA (real-time-PCR) were associated with BAL eosinophilia. Pulmonary eosinophilia abated without treatment apart from deworming. It appears that pronounced lung eosinophilia in horses can be transient, abate without specific treatment, and in this instance, lack correlation to upregulation of expression of either IL-4 or IL-5.
authors.affiliation: Section of Equine Internal Medicine, Department of Clinical Sciences, Box 7054, 750 07 Uppsala, Sweden. Miia.Riihimaki@kv.slu.se
authors.forename: M
authors.initials: M
authors.lastname: Riihimäki
authors.forename: I
authors.initials: I
authors.lastname: Lilliehöök
authors.forename: A
authors.initials: A
authors.lastname: Raine
authors.forename: M
authors.initials: M
authors.lastname: Berg
authors.forename: J
authors.initials: J
authors.lastname: Pringle
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D004804:Eosinophils / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006734:Horse Diseases / Q000378:metabolism*; D006736:Horses; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism*; D015848:Interleukin-5 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009504:Neutrophils / Q000378:metabolism; D011657:Pulmonary Eosinophilia / Q000378:metabolism / Q000662:veterinary*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D015848:Interleukin-5; D012333:RNA, Messenger; D015847:Interleukin-4