Binding interaction of [Re(H2O)3(CO)3]+ with the DNA fragment d(CpGpG).
Insights into the interaction of the [Re(H2O)3(CO)3]+ complex (1) with the DNA fragment d(CpGpG) have been obtained by one- (1D) and two-dimensional (2D) NMR spectroscopy. The H8 resonances of the single major [Re(H2O)d(CpGpG)(CO)3]- adduct (2) exhibit pH-independent chemical shift changes attributable to metal N7 binding. The structure of this adduct has been characterized by molecular modeling studies based on 1D and 2D NMR data. In solution, 2 shows the presence of two N7-coordinated guanine moieties in a head-to-head (HH) orientation as evidenced by G2H8/G3H8 cross-peaks in the 1H-1H NOESY NMR spectrum. The presence of the 5'-bridging phosphodiester appears to stabilize the HH1 L conformer, as was previously described for related Pt and Rh complexes.
Zobi
Fabio
F
Institute of Inorganic Chemistry, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland. fzobi@aci.unizh.ch
Blacque
Olivier
O
Sigel
Roland K O
RK
Alberto
Roger
R
D001483:Base Sequence; D002254:Carbonates / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D015394:Molecular Structure; D012211:Rhenium / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D002254:Carbonates; D014867:Water; D012211:Rhenium; D004247:DNA