title: A combination of octanoate and oleate promotes in vitro differentiation of porcine intramuscular adipocytes.
abstract: To understand the relationship between intramuscular adipogenesis in the pig and the supply fatty acids, we established a clonal porcine intramuscular preadipocyte (PIP) line from the marbling muscle tissue of female Duroc pig. Confluent PIP cells exhibited a fibroblastic appearance. Their adipogenic ability was investigated using confluent PIP cells after exchanging growth medium for adipogenic medium containing 50 ng/mL insulin, 0.25 microM dexamethasone, 2 mM octanoate, and 200 microM oleate. Appropriate concentrations of octanoate and oleate for the induction of adipogenesis were determined from the ability of cells to accumulate lipid and the toxicity of fatty acids. When cells were cultured in differentiation medium for 8 days, large numbers of lipid droplets were observed in differentiated PIP cells, and their cytosolic TG content increased in a time-dependent manner. While oleate only induced the expression of PPARgamma mRNA, but not that of C/EBPalpha, octanoate significantly induced the expression of both PPARgamma and C/EBPalpha mRNA. Octanoate and oleate accelerated the inducing effect of insulin and dexamethasone on the expression of aP2 mRNA. These results indicate that a combination of octanoate and oleate synergistically induced PIP adipogenesis, and that the stimulation of octanoate was essential to the trigger for the adipogenesis in PIP cells.
authors.affiliation: Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-amamiyamachi 1-1, Aoba-ku, Sendai, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Sanosaka
authors.forename: Takeshi
authors.initials: T
authors.lastname: Minashima
authors.forename: Keiichi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Kouichi
authors.initials: K
authors.lastname: Watanabe
authors.forename: Shyuichi
authors.initials: S
authors.lastname: Ohwada
authors.forename: Akihiko
authors.initials: A
authors.lastname: Hagino
authors.forename: Michael T
authors.initials: MT
authors.lastname: Rose
authors.forename: Takahiro
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Aso
mesh_terms: D052438:Adipocytes, White / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002210:Caprylates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D004338:Drug Combinations; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D018482:Muscle, Skeletal / Q000737:chemistry / Q000187:drug effects / Q000502:physiology; D019301:Oleic Acid / Q000494:pharmacology*; D034421:Sus scrofa
chemical_list: D015415:Biomarkers; D002210:Caprylates; D004338:Drug Combinations; D019301:Oleic Acid; C031492:octanoic acid