title: Pyrosequencing for discovery and analysis of DNA sequence variations.
abstract: Since the invention of pyrosequencing, more than 500 articles have been published describing different applications of this technology, most notably for DNA structure variation and microbial detection. Technological advances have been made to enhance the robustness and accuracy of this technique as well as to reduce the cost and increase the throughput. This review intends to cover recent advances in this technology and discuss its application for low and high-throughput DNA variation studies.
authors.affiliation: Stanford Genome Technology Center, Stanford University, 855 California Avenue, Palo Alto, CA 94304, USA. mostafa@stanford.edu
authors.forename: Mostafa
authors.initials: M
authors.lastname: Ronaghi
authors.forename: Shadi
authors.initials: S
authors.lastname: Shokralla
authors.forename: Baback
authors.initials: B
authors.lastname: Gharizadeh
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D014644:Genetic Variation; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods*; D013698:Templates, Genetic
chemical_list: D004247:DNA