A non-invasive, in vivo technique for monitoring vascular status of glioblastoma during angiogenesis.
The growth of solid tumors dependent on the process of angiogenesis in which growth factors secreted by tumor and stromal cells promote endothelial cell proliferation, migration, and maturation. This process generates a tumor-specific vascular supply and enables small or dormant tumors to grow rapidly with exponential increases in tumor volume. Determination of tumor oxygenation at the microvascular level will provide important insight into tumor growth, angiogenesis, necrosis, and therapeutic response, and will facilitate to develop protocols for studying tumor behavior. A non-invasive multi-modality approach based on near infrared spectroscopy (NIRS) technique, namely: Steady State Diffuse Optical Spectroscopy (SSDOS) along with Magnetic Resonance Imaging (MRI) is applied for monitoring the concentration of oxyhemoglobin, deoxyhemoglobin and water within tumor region and for studying the vascular status of tumor and the patho-physiological changes that occur during angiogenesis. Since, the growth of solid tumors depends on the formation of new blood vessels, an association between intramural microvessel density (MVD) and tumor oxygenation is also investigated. The relative decrease in oxygenation value with tumor growth indicates that though blood vessels infiltrate and proliferate the tumor region, a hypoxic trend is clearly present.
Saxena
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Department of Radiology, Keck School of Medicine and Children's Hospital Los Angeles, University of Southern California, Los Angeles, California 90027, USA. VSaxena@usc.edu
Gonzalez-Gomez
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Laug
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D000465:Algorithms; D000818:Animals; D001932:Brain Neoplasms / Q000098:blood supply* / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D005909:Glioblastoma / Q000098:blood supply* / Q000378:metabolism; D006801:Humans; D008279:Magnetic Resonance Imaging; D051379:Mice; D008819:Mice, Nude; D008962:Models, Theoretical; D009389:Neovascularization, Pathologic / Q000175:diagnosis*; D055095:Optics and Photonics; D010108:Oxyhemoglobins / Q000032:analysis; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D014867:Water / Q000032:analysis
D010108:Oxyhemoglobins; D014867:Water