Amycolatopsis regifaucium sp. nov., a novel actinomycete that produces kigamicins.
The taxonomic position of seven soil actinomycetes provisionally assigned to the genus Amycolatopsis was established in a polyphasic study. The isolates, which had identical 16S rRNA gene sequences, had closest similarity to the type strain of Amycolatopsis orientalis. A representative isolate, strain GY080T, had chemotaxonomic properties that were typical of the genus Amycolatopsis and could be distinguished from the type strain of A. orientalis using DNA-DNA relatedness data. All of the isolates shared a phenotypic profile that distinguished them from representatives of phylogenetically closely related species. Amplified rDNA restriction analysis showed that the isolates formed a homogeneous group that was distinctly separate from single-membered groups consisting of representative Amycolatopsis type strains, including that of A. orientalis. Based on the combined genotypic and phenotypic evidence, it is proposed that the seven isolates be classified as representatives of a novel species for which the name Amycolatopsis regifaucium sp. nov. is proposed. The type strain is GY080T (=DSM 45072T=NCIMB 14277T).
Tan
Geok Yuan Annie
GYA
School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
Robinson
Stuart
S
Microbial Screening Technologies, Building A, 28-54 Percival Road, Smithfield, New South Wales 2164, Australia.
Lacey
Ernest
E
Microbial Screening Technologies, Building A, 28-54 Percival Road, Smithfield, New South Wales 2164, Australia.
Brown
Roselyn
R
School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
Kim
Wonyong
W
School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
Goodfellow
Michael
M
School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001315:Australia; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000378:metabolism*; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010080:Oxazoles / Q000378:metabolism*; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010080:Oxazoles; D012336:RNA, Ribosomal, 16S; D004317:Doxorubicin