Rapid diagnosis of human brucellosis by quantitative real-time PCR: a case report of brucellar spondylitis.
Blood samples and a bone biopsy specimen from one patient diagnosed with spondylodiscitis of unknown etiology were analyzed by quantitative real-time PCR to detect Brucella melitensis. The high sensitivity and specificity of this assay allowed the diagnosis of brucellar spondylitis within 24 h, a result that we were unable to obtain through the use of conventional methods.
Navarro-Martínez
Amalia
A
Internal Medicine Department, University General Hospital, c/ Hermanos Falcó s/n, 02006, Albacete, Spain.
Navarro
Elena
E
Castaño
Maria Jesús
MJ
Solera
Javier
J
D000369:Aged, 80 and over; D001769:Blood / Q000382:microbiology; D001853:Bone Marrow / Q000382:microbiology; D017347:Brucella melitensis / Q000235:genetics / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis*; D005260:Female; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D013166:Spondylitis / Q000382:microbiology*