The assessment of T-cell apoptosis in synovial fluid.
T-cell apoptosis is central to the resolution of chronic inflammation. Inhibition of this process of programmed cell death contributes to disease persistence in conditions such as rheumatoid arthritis. An understanding of T-cell apoptosis and its regulation is clearly important for understanding the pathophysiology of inflammatory disease. This chapter describes a number of apoptosis assays that can be used to measure T-cell apoptosis in synovial fluid. The choice of assay depends, in part, on the phase of apoptosis under investigation and this review puts this into context by introducing these phases and their regulation.
Raza
Karim
K
MRC Centre for Immune Regulation, Institute for Biomedical Research Building, University of Birmingham, UK.
Scheel-Toellner
Dagmar
D
Lord
Janet M
JM
Akbar
Arne N
AN
Buckley
Christopher D
CD
Salmon
Mike
M
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001681:Biological Assay / Q000379:methods*; D017252:CD3 Complex / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D053938:DNA Fragmentation; D006801:Humans; D020287:In Situ Nick-End Labeling; D007249:Inflammation / Q000276:immunology; D008928:Mitochondria / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D013582:Synovial Fluid / Q000166:cytology* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology*
D017252:CD3 Complex; D010718:Phosphatidylserines; D011956:Receptors, Cell Surface; D004247:DNA; D053148:Caspase 3