Serogrouping of non-interpretable Neisseria meningitidis carriage strains, using rapid diagnostic tests.
Large-scale Neisseria meningitidis (Nm) carriage studies in Africa are hampered by the lack of easy-to-perform and reliable methods for serogrouping strains that are largely polyagglutinable or autoagglutinable isolates using the conventional agglutination method. We tested the recently developed duplex rapid diagnostic tests (RDT1 Nm A and Y/W135, RDT2 Nm C and Y) for the serogrouping of 55 non-interpretable carriage strains. Thirteen (23.6%) could be serogrouped, of which nine were serogroup W135. Rapid diagnostic tests are a useful and efficient tool for the identification and serogrouping of Nm for carriage studies.
Hamidou
Amina Amadou
AA
Centre de Recherche Médicale et Sanitaire (CERMES), YN034, BP 10887, Niamey, Niger. amina@cermes.org
Djibo
Saacou
S
Mamane
Ali Elhadj
AE
Moussa
Amadou
A
Chanteau
Suzanne
S
D000349:Africa; D000372:Agglutination Tests; D002353:Carrier State / Q000382:microbiology; D006801:Humans; D008585:Meningitis, Meningococcal / Q000276:immunology / Q000382:microbiology*; D009345:Neisseria meningitidis / Q000145:classification* / Q000276:immunology; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012703:Serotyping / Q000379:methods*
D011933:Reagent Kits, Diagnostic