Hybridization analysis using oligonucleotide probe arrays.
This chapter describes methodology for the labeling, hybridization, and detection of amplicon target DNA to arrays of oligonucleotide probes attached to plastic substrates. A systematic approach to target discrimination based on both hybridization and wash stringency is provided.
Matson
Robert S
RS
Beckman Coulter, Inc., Fullerton, CA, USA.
Rampal
Jang B
JB
D052160:Benzothiazoles; D001710:Biotin / Q000378:metabolism; D003550:Cystic Fibrosis / Q000235:genetics; D003959:Diamines; D004586:Electrophoresis; D006801:Humans; D009154:Mutation / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000592:standards; D015345:Oligonucleotide Probes / Q000378:metabolism*; D009930:Organic Chemicals / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011804:Quinolines; D013194:Staining and Labeling
D052160:Benzothiazoles; D003959:Diamines; D015345:Oligonucleotide Probes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D001710:Biotin