Rapid determination of chloramphenicol residues in aquaculture tissues by immunochromatographic assay.
An immunochromatographic assay was developed to detect chloramphenicol (CAP) residues in aquaculture tissues. The limit of detection (LOD) was 10 ng g(-1) for detecting CAP spiked in the aquaculture tissues. The results were confirmed by liquid chromatography tandem mass spectrometry (LC/MS/MS) and indicated that there was a good agreement between the two methods. The linear regression equation was y = 1.19x + 0.539 with R(2) = 0.978. The assay time for test was less than 5 min and the method is suitable for rapid testing on-site.
Li
Ke
K
WuXi KangBaiEr Biotechnology Corporation, WuXi, JiangSu, P.R. China. keli81@hotmail.com
Liu
Liqiang
L
Xu
ChuanLai
C
Chu
XiaoGang
X
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D000906:Antibodies / Q000276:immunology; D017756:Aquaculture; D002478:Cells, Cultured; D002701:Chloramphenicol / Q000032:analysis*; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods*; D003429:Cross Reactions / Q000276:immunology; D005260:Female; D005399:Fishes; D007118:Immunoassay / Q000379:methods*; D016014:Linear Models; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry; D013997:Time Factors
D000900:Anti-Bacterial Agents; D000906:Antibodies; D002701:Chloramphenicol