An effective method for detection and analysis of DNA damage induced by heavy-ion beams.
We have developed an efficient system to detect and analyze DNA mutations induced by heavy-ion beams in Arabiopsis thaliana. In this system, a stable transgenic Arabidopsis line that constitutively expresses a yellow fluorescent protein (YFP) by a single-copy gene at a genomic locus was constructed and irradiated with heavy-ion beams. The YFP gene is a target of mutagenesis, and its loss of function or expression can easily be detected by the disappearance of YFP signals in planta under microscopy. With this system, a (12)C(6+)-induced mutant with single deletion and multiple base changes was isolated.
Kazama
Yusuke
Y
RIKEN Nishina Center, Saitama 351-0198, Japan.
Saito
Hiroyuki
H
Fujiwara
Makoto
M
Matsuyama
Tomoki
T
Hayashi
Yoriko
Y
Ryuto
Hiromichi
H
Fukunishi
Nobuhisa
N
Abe
Tomoko
T
D017360:Arabidopsis / Q000235:genetics* / Q000528:radiation effects*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004252:DNA Mutational Analysis / Q000379:methods*; D017930:Genes, Reporter; D020450:Heavy Ions; D008164:Luminescent Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009154:Mutation; D030821:Plants, Genetically Modified / Q000235:genetics / Q000528:radiation effects
D001426:Bacterial Proteins; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria; D004247:DNA