Expression of extracellular matrix proteins in the vocal folds and bone marrow derived stromal cells of rats.
Vocal fold scarring remains a therapeutic challenge. Our research group has indicated that bone marrow-derived stromal cells (BSCs) may have therapeutic potential in restoration of injured vocal folds. However, it is still unclear how BSCs restore the viscoelasticity of vocal fold mucosa. Since a feature of vocal fold scarring is the disorganization of the extracellular matrix (ECM), it is important to understand how BSCs produce ECM. The present study aimed to clarify ECM gene expression in BSCs, and also examined the effects of hepatocyte growth factor (HGF) on this expression. BSCs obtained from the femurs of four Sprague-Dawley rats were cultured with or without HGF. The mRNA expression of ECM components (type I procollagen, decorin, Has2, CD44, MMP-1, and GAPDH) were examined in cultured BSCs and the vocal fold mucosa by the reverse transcription-polymerase chain reaction (RT-PCR). The mRNA expression of Has2 and MMP-1 was significantly stronger in BSCs than in the vocal folds (P < 0.05). Expression of Has2 in BSCs was significantly increased by the administration of HGF (P < 0.05). There was no significant difference in the gene expression of other ECM molecules between BSCs and vocal fold mucosa. Increased expression of Has2 and MMP-1 genes from BSCs may have a positive potential in the treatment of vocal fold scarring.
Ohno
Tsunehisa
T
Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Sakyo-ku, 606-8507 Kyoto, Japan. tohno@ent.kuhp.kyoto-u.ac.jp
Hirano
Shigeru
S
Kanemaru
Shin-ichi
S
Yamashita
Masaru
M
Umeda
Hiroo
H
Suehiro
Atsushi
A
Nakamura
Tatsuo
T
Ito
Juichi
J
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D004195:Disease Models, Animal; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D014453:Glucuronosyltransferase / Q000096:biosynthesis / Q000235:genetics; D000076002:Hyaluronan Synthases; D007818:Laryngeal Diseases / Q000235:genetics / Q000378:metabolism / Q000601:surgery; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033581:Stem Cell Transplantation / Q000379:methods*; D017154:Stromal Cells / Q000166:cytology* / Q000637:transplantation; D014827:Vocal Cords / Q000378:metabolism* / Q000601:surgery
D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D014453:Glucuronosyltransferase; C491097:Has2 protein, rat; D000076002:Hyaluronan Synthases; D020781:Matrix Metalloproteinase 1