The role of nickel accumulation and epithelial cell proliferation in orthodontic treatment-induced gingival overgrowth.
The aim of this study was to investigate the role of nickel in orthodontic treatment-induced gingival hyperplasia. The nickel concentration in gingival tissues with and without overgrowth, histopathology of gingival overgrowth, and epithelial cell proliferation response to different nickel concentrations were analysed. Ten patients receiving orthodontic therapy (eight females and two males, mean age 15.4 years) were included in the study. Hyperplastic and healthy gingiva samples were collected from the same patients. The amount of nickel in the gingival tissue samples was analysed using the atomic absorption spectrometry technique. The tissues removed from hyperplastic areas during gingivectomy were also used for histological analysis. To analyse the effect of nickel on epithelial cell proliferation, four different nickel concentrations (0.5, 2, 5, and 10 microg) were incubated with keratinocyte cells for 11 days. Mann-Whitney U-test, analysis of variance, and Tukey's test were used in the statistical analyses. The results did not show any difference in nickel concentration between the study and control gingiva tissue samples, but histological analysis demonstrated an increase in epithelial thickness and a significant increase (P = 0.031, 0.02, 0.02) in epithelial cell proliferation in response to low-dose nickel concentrations, with a toxic response to a higher dose. In the limitations of this study, it is plausible that the effect of a continuing low-dose nickel release to epithelium is the initiating factor of gingival overgrowth induced by orthodontic treatment.
Gursoy
Ulvi Kahraman
UK
Department of Oral and Maxillofacial Surgery, Helsinki University Institute of Dentistry, Helsinki University Central Hospital, Finland. ulvi.gursoy@helsinki.fi
Sokucu
Oral
O
Uitto
Veli-Jukka
VJ
Aydin
Ahmet
A
Demirer
Serhat
S
Toker
Hulya
H
Erdem
Onur
O
Sayal
Ahmet
A
D000293:Adolescent; D000328:Adult; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003722:Dental Alloys; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D005260:Female; D005881:Gingiva / Q000187:drug effects / Q000473:pathology*; D005885:Gingival Hyperplasia / Q000473:pathology; D019214:Gingival Overgrowth / Q000473:pathology*; D005890:Gingivectomy; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000473:pathology; D008297:Male; D009532:Nickel / Q000009:adverse effects / Q000032:analysis*; D009967:Orthodontic Appliances / Q000009:adverse effects*; D016910:Orthodontic Brackets; D009969:Orthodontic Wires; D013054:Spectrophotometry, Atomic; D013193:Stainless Steel; D014025:Titanium
D003722:Dental Alloys; C040654:titanium nickelide; D013193:Stainless Steel; D009532:Nickel; D014025:Titanium