Identification of Nocardia farcinica by a PCR primer amplifying a specific DNA band for the bacterium.
A PCR primer specific to Nocardia farcinica was prepared based on sequence information of random amplified polymorphic DNA (RAPD) analysis. The PCR primer amplifies N. farcinica species only; no amplification was observed in 25 other Nocardia strains that we tested. Specificity of the primer for N. farcinica was also confirmed using other fungal and bacterial strains that are frequently isolated from clinical samples such as sputa and broncho alveolar lavage (VAL).
Hasegawa
Taichi
T
Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
Gonoi
Tohru
T
Ito
Junji
J
Kogure
Takahisa
T
Yazawa
Katsukiyo
K
Mikami
Yuzuru
Y
D000818:Animals; D001769:Blood / Q000382:microbiology; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D051379:Mice; D009615:Nocardia / Q000235:genetics* / Q000302:isolation & purification*; D009617:Nocardia Infections / Q000175:diagnosis* / Q000382:microbiology*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D013183:Sputum / Q000382:microbiology
D017931:DNA Primers; D004269:DNA, Bacterial