Implementation of electrochemiluminescence microanalysis in PCB technology.
We present an instrumental development to implement electrochemiluminescence (ECL) microanalysis using printed circuit board (PCB) technology. PCB gold macro-(10 mm2) and micro- (0.09 mm2) electrodes and two ECL microfluidic devices are designed, fabricated and tested via luminol ECL detection. Potential modulation is performed between 0.7 and 0 V vs. Ag/AgCl for luminol oxidation, thus giving rise to on/off ECL responses in the presence of hydrogen peroxide. Synchronous detection is adopted to allow weak ECL signal recovery at a very low signal-to-noise ratio (SNR). The detection limit obtained with the two ECL microfluidic devices is 50 nM and 100 nM H2O2 for macroelectrodes and microelectrodes, respectively.
Pittet
Patrick
P
INL Institut des Nanotechnologies de Lyon, 43 boulevard du 11 novembre 1918, Villeurbanne, F-69622, France. Patrick.Pittet@lenac.univ-lyon1.fr
Lu
Guo-Neng
GN
Galvan
Jean-Marc
JM
Ferrigno
Rosaria
R
Stephan
Khaled
K
Blum
Loïc J
LJ
Leca-Bouvier
Béatrice D
BD
D004563:Electrochemistry; D004581:Electronics; D006861:Hydrogen Peroxide / Q000737:chemistry; D049408:Luminescent Agents / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques; D012834:Silver / Q000737:chemistry; D018030:Silver Compounds / Q000737:chemistry
D049408:Luminescent Agents; D018030:Silver Compounds; D012834:Silver; D008165:Luminol; D006861:Hydrogen Peroxide; C037548:silver chloride