Dietary administration of sodium alginate and kappa-carrageenan enhances the innate immune response of brown-marbled grouper Epinephelus fuscoguttatus and its resistance against Vibrio alginolyticus.
Brown-marbled grouper Epinephelus fuscoguttatus which had been fed diets containing sodium alginate and kappa (kappa)-carrageenan at 5, 10 and 20gkg(-1), respectively after 0, 2, 4, 6, 8 and 14 weeks were examined for survival, growth, innate cellular and humoral responses as compared to the fish that fed control non-supplemented diet. Survival was 100% for the fish that fed all diets after 14 weeks and no significant difference in growth was observed among seven diets. The fish that fed a diet containing sodium alginate at 10gkg(-1), and fed a diet containing kappa-carrageenan at 5gkg(-1) over 2-8 weeks showed significantly increased leucocyte count, respiratory burst, phagocytic activity and phagocytic index, The fish that fed a diet containing sodium alginate at 5gkg(-1), and fed a diet containing kappa-carrageenan at 5gkg(-1) over 2-8 weeks showed significantly increased alternative complement activity (ACH50) and lysozyme activity. In another experiment, E. fuscoguttatus, which had been fed control diet, and all diets containing sodium alginate, and kappa-carrageenan after 14 weeks were challenged with Vibrio alginolyticus at 5.0x10(9) colony-forming units (cfu) fish(-1) and then placed in seawater of 34 per thousand. The survival of fish that fed a diet containing sodium alginate at 10gkg(-1) and kappa-carrageenan at 5gkg(-1) was significantly higher than that of fish which fed the control diet over 96-168h. It was concluded that E. fuscoguttatus which fed a diet containing sodium alginate at 10gkg(-1) or less, or fed a diet containing kappa-carrageenan at 5gkg(-1) enhance the innate immunity, and increase the resistance from V. alginolyticus infection.
Cheng
Ann-Chang
AC
Department of Aquaculture, National Penghu University, Makong, Penghu 880, Taiwan, Republic of China.
Chen
Yu-Yuan
YY
Chen
Jiann-Chu
JC
D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D001492:Bass / Q000276:immunology*; D002351:Carrageenan / Q000494:pharmacology*; D003170:Complement Pathway, Alternative; D019587:Dietary Supplements; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology*; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D007113:Immunity, Innate / Q000276:immunology; D007958:Leukocyte Count / Q000662:veterinary; D010587:Phagocytosis; D016897:Respiratory Burst; D014735:Vibrio Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D044149:Vibrio alginolyticus / Q000276:immunology*
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D002351:Carrageenan