Immunization of macaques with formalin-inactivated human metapneumovirus induces hypersensitivity to hMPV infection.
Human metapneumovirus (hMPV), a member of the family Paramyxoviridae, is an important cause of acute respiratory tract disease. In the 1960s, vaccination with formalin-inactivated paramyxovirus preparations--respiratory syncytial virus (RSV) and measles virus (MV)--resulted in predisposition for enhanced disease upon natural infection. We have produced a formalin-inactivated hMPV preparation (FI-hMPV), which was used to immunize young cynomolgus macaques. Six days after challenge FI-hMPV-primed monkeys had developed eosinophilic bronchitis and bronchiolitis, indicative of a hypersensitivity response. This study indicates that formalin-inactivated hMPV vaccines have the same propensity to predispose for immune-mediated disease as inactivated RSV and MV vaccines.
de Swart
Rik L
RL
Department of Virology, Erasmus MC, P.O. Box 2040, 3000 CA Rotterdam, The Netherlands. r.deswart@erasmusmc.nl
van den Hoogen
Bernadette G
BG
Kuiken
Thijs
T
Herfst
Sander
S
van Amerongen
Geert
G
Yüksel
Selma
S
Sprong
Leo
L
Osterhaus
Albert D M E
AD
D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005557:Formaldehyde; D007074:Immunoglobulin G / Q000032:analysis / Q000302:isolation & purification; D007371:Interferon-gamma / Q000097:blood; D008168:Lung / Q000473:pathology / Q000821:virology; D008214:Lymphocytes / Q000187:drug effects; D008252:Macaca fascicularis; D008297:Male; D029121:Metapneumovirus / Q000276:immunology*; D018184:Paramyxoviridae Infections / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology*; D015164:Vaccines, Inactivated / Q000009:adverse effects; D014765:Viral Vaccines / Q000009:adverse effects*
D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000914:Antibodies, Viral; D016207:Cytokines; D007074:Immunoglobulin G; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D005557:Formaldehyde; C041524:aluminum sulfate; D007371:Interferon-gamma