[Study on phytoremediation of phenanthrene-contaminated soil with alfalfa (Medicago sativa L.)].
Pot experiment was used to investigate phytoremediation of phenanthrene-contaminated soil with alfalfa (Medicago sativa L.). Results indicated that phenanthrene had inhibitive effect on alfalfa growth, and higher phenanthrene concentration seriously prevent alfalfa growth. When the concentration was 445.22 mg/kg, the shoot and root biomasses were only 57.31% and 31.20% of control respectively. Alfalfa significantly promoted phenanthrene degradation in the soil. After 60 days, 85.68%-91.40% and 75.25%-86.61% of spiked phenanthrene disappeared from the rhizosphere and non-rhizosphere soils respectively. And the average removal ratio of phenanthrene in rhizosphere soils was 6.33% higher than that in non-rhizoshpere soils. The residual concentration of phenanthrene in the rhizosphere was lower than that in the non-rhizosphere but the dehydrogenase activity was on the contrary. With phenanthrene concentration increase the removal ratio and dehydrogenase activity decreased. A positive correlation was observed between the soil dehydrogenase activity and the removal ratio of phenanthrene in both the rhizosphere and non-rhizosphere soils. Therefore the presence of alfalfa roots was effective in promoting the phytoremediation of phenanthrene.
Fan
Shu-xiu
SX
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
Li
Pei-jun
PJ
Gong
Zong-qiang
ZQ
He
Na
N
Zhang
Li-hong
LH
Ren
Wan-xia
WX
Verkhozina
V A
VA
D001673:Biodegradation, Environmental; D018533:Biomass; D004784:Environmental Monitoring; D052918:Environmental Restoration and Remediation / Q000379:methods; D000455:Medicago sativa / Q000254:growth & development / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D010616:Phenanthrenes / Q000032:analysis / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
D010616:Phenanthrenes; D012989:Soil Pollutants; D010088:Oxidoreductases