PLAC1, a trophoblast-specific cell surface protein, is expressed in a range of human tumors and elicits spontaneous antibody responses.
Identification of genes that are upregulated in tumors, and whose normal expression excludes adult somatic tissues but includes germline and/or embryonic tissues, has resulted in a rich variety of cancer antigens that are attractive targets for cancer vaccine and other therapeutic approaches. In the present study, we extended this approach to include genes strongly and restrictively expressed in the placenta by mining publicly available SAGE and EST databases. We identified a number of genes with high expression in placenta and different cancer types but with relatively restricted expression in normal tissues. The gene with the most distinctive expression pattern was found to be PLAC1, which encodes a putative cell surface protein that is highly expressed in placenta, testis, cancer cell lines and lung tumors. Hence we have designated it CT92. We found by ELISA that PLAC1 is immunogenic in a subset of cancer patients and healthy women. Its physical and expression characteristics render it a potential target for both active and passive cancer immunotherapeutic strategies.
Silva
Wilson A
WA
Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Gnjatic
Sacha
S
Ritter
Erika
E
Chua
Ramon
R
Cohen
Tzeela
T
Hsu
Melinda
M
Jungbluth
Achim A
AA
Altorki
Nasser K
NK
Chen
Yao-Tseng
YT
Old
Lloyd J
LJ
Simpson
Andrew J G
AJ
Caballero
Otavia L
OL
D000912:Antibodies, Neoplasm / Q000097:blood*; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D011257:Pregnancy Proteins / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000378:metabolism
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; C415583:PLAC1 protein, human; D011257:Pregnancy Proteins