Ginsenoside Rd production from the major ginsenoside Rb(1) by beta-glucosidase from Thermus caldophilus.
Under optimum conditions (pH 5, 75 degrees C, and 0.2 U purified enzyme ml(-1)), 4 mg ginsenoside Rd was produced from 5 mg reagent-grade ginsenoside Rb(1) in 5 ml after 30 min by beta-glucosidase from Thermus caldophilus GK24. Using a ginseng root extract containing 1 mg ginsenoside Rb(1) ml(-1) and 3.2 mg additional ginsenosides ml(-1), 1.23 mg ginsenoside Rd ml(-1) was produced after 18 h; the concentrations of ginsenosides Rb(1), Rb(2), and Rc used for ginsenoside Rd production were 0.77, 0.17, and 0.19 mg ml(-1), respectively.
Son
Ju-Wan
JW
Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, South Korea.
Kim
Hye-Jung
HJ
Oh
Deok-Kun
DK
D001426:Bacterial Proteins / Q000378:metabolism*; D036145:Ginsenosides / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D005894:Panax / Q000737:chemistry; D010936:Plant Extracts / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D013696:Temperature; D013824:Thermus / Q000201:enzymology*; D001617:beta-Glucosidase / Q000378:metabolism*
D001426:Bacterial Proteins; D036145:Ginsenosides; D010936:Plant Extracts; D001617:beta-Glucosidase; C049863:ginsenoside Rd