Designing Zn(II) and Cu(II) derivatives as probes for in vitro fluorescence imaging.
New M(II) bis(thiosemicarbazonato) complexes (M = Ni(II), Cu(II) and Zn(II)) featuring allyl groups at the exocyclic nitrogens have been synthesised. The complexes were characterised in solution by spectroscopic methods and their solid state structures determined by single crystal X-ray diffraction using synchrotron radiation. The Zn(II) complex was found to be intrinsically fluorescent and soluble in biocompatible media. The uptake of this Zn(II) complex in HeLa, MCF-7 and IGROV cancer cells was monitored by fluorescence microscopies (epi- and confocal fluorescence imaging). The radiolabelling to (64)Cu(II) bis(thiosemicarbazonato) complex was performed cleanly by transmetallation from the corresponding Zn(II) species using (64)Cu(OAc)(2).
Pascu
Sofia I
SI
Chemistry Research Laboratory, Universiy of Oxford, 12 Mansfield Road, Oxford, UKOX1 3TA. sofia.pascu@chem.ox.ac.uk
Waghorn
Philip A
PA
Conry
Timothy D
TD
Betts
Helen M
HM
Dilworth
Jonathan R
JR
Churchill
Grant C
GC
Pokrovska
Tzveta
T
Christlieb
Martin
M
Aigbirhio
Franklin I
FI
Warren
John E
JE
D045744:Cell Line, Tumor; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008856:Microscopy, Fluorescence / Q000379:methods*; D015335:Molecular Probes; D015032:Zinc / Q000737:chemistry*
D015335:Molecular Probes; D003300:Copper; D015032:Zinc