Immunological properties of human embryonic stem cell-derived oligodendrocyte progenitor cells.
A major concern in the use of allotransplantation of human embryonic stem cell (hESC)-based therapies is the possibility of allogeneic rejection by the host's immune system. In this report, we determined the immunological properties of hESC-derived oligodendrocyte progenitor cells (OPC) that have the potential for clinical application for the treatment of patients with spinal cord injury. In vitro immunological studies suggest that hESC-derived OPCs are poor targets for both the innate and the adaptive human immune effector cells as well as resistant to lysis by anti-Neu5Gc antibodies. These results indicate that hESC-derived OPCs retain some of the unique immunological properties of the parental cell line from which they were differentiated.
Okamura
Ross M
RM
Geron Corporation, Menlo Park, California, USA. rokamura@geron.com
Lebkowski
Jane
J
Au
Melinda
M
Priest
Catherine A
CA
Denham
Jerrod
J
Majumdar
Anish S
AS
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis; D004268:DNA-Binding Proteins / Q000172:deficiency; D053595:Embryonic Stem Cells / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005333:Fetus; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D016513:Mice, SCID; D009836:Oligodendroglia / Q000276:immunology*; D012794:Sialic Acids / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism; D033581:Stem Cell Transplantation / Q000379:methods; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D016207:Cytokines; D004268:DNA-Binding Proteins; C078888:N-(O-acetyl)glycoloylneuraminic acid; C499308:Rag2 protein, mouse; D012794:Sialic Acids