Mechanisms contributing to tonic release at the cone photoreceptor ribbon synapse.
Time-resolved capacitance measurements in combination with fluorescence measurements of internal calcium suggested three kinetic components of release in acutely isolated cone photoreceptors of the tiger salamander. A 45-fF releasable pool, corresponding to about 1,000 vesicles, was identified. This pool could be depleted with a time constant of a few hundred milliseconds and its recovery from depletion was quite rapid (tau approximately 1 s). The fusion of vesicles in this pool was blocked by low-millimolar EGTA. Endocytosis was sufficiently slow that it is likely that refilling of the releasable pool occurred from preformed vesicles. A second, slower component of release (tau(depletion) approximately 3 s) was identified that was approximately twice the size of the releasable pool. This pool may serve as a first reserve pool that replenishes the releasable pool. Computer simulations indicate that the properties of the releasable and first reserve pools are sufficient to maintain synaptic signaling for several seconds in the face of near-maximal stimulations and in the absence of other sources of vesicles. Along with lower rates of depletion, additional mechanisms, such as replenishment from distal reserve pools and the fast recycling of vesicles, may further contribute to the maintenance of graded, tonic release from cone photoreceptors.
Innocenti
Barbara
B
The W. M. Keck Center for the Neurobiology of Learning and Memory, University of Texas Medical School at Houston, 6431 Fannin Street, Houston, TX 77025, USA. ruth.heidelberger@uth.tmc.edu
Heidelberger
Ruth
R
D000557:Ambystoma; D000818:Animals; D020013:Calcium Signaling / Q000502:physiology; D003198:Computer Simulation; D004558:Electric Stimulation; D004705:Endocytosis / Q000502:physiology; D005089:Exocytosis / Q000502:physiology; D018698:Glutamic Acid / Q000378:metabolism*; D008561:Membrane Fusion / Q000502:physiology; D018408:Patch-Clamp Techniques; D011930:Reaction Time / Q000502:physiology; D012160:Retina / Q000502:physiology* / Q000648:ultrastructure; D017949:Retinal Cone Photoreceptor Cells / Q000502:physiology* / Q000648:ultrastructure; D013569:Synapses / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology*; D013572:Synaptic Vesicles / Q000378:metabolism*; D013997:Time Factors; D014785:Vision, Ocular / Q000502:physiology
D018698:Glutamic Acid