Posttranscriptional regulation of FLO11 upon amino acid starvation in Saccharomyces cerevisiae.
Various starvation conditions cause adhesive growth of haploid cells or pseudohyphae formation of diploid cells of Saccharomyces cerevisiae. For the genetic Sigma1278b background, these morphological changes depend on the expression of the gene encoding the cell wall glycoprotein Flo11p, which is increased during nutritional limitations. Deletion of the genes encoding the transcriptional coactivators Rsc1p or Gcn5p impairs FLO11 transcription, which consequently leads to a loss of both haploid invasive growth and diploid pseudohyphae development upon glucose and nitrogen limitation, respectively. In contrast, amino acid starvation induces FLO11-dependent adhesive growth of the rsc1Delta and gcn5Delta strains although FLO11 transcription remains very low. The double deletion strain rsc1Deltaflo11Delta, however, does not grow adhesively, suggesting that the adhesion of the rsc1Delta strain at amino acid starvation is still FLO11-dependent. The FLO11prom-lacZ-encoded beta-galactosidase activities of the rsc1Delta and gcn5Delta mutant strains increase manifold upon amino acid starvation. It is therefore concluded that low levels of FLO11 transcripts are essential and sufficient for derepression of FLO11 expression and adhesive growth during amino acid starvation. A posttranscriptional control is assumed to be behind this phenomenon that permits the increased FLO11 expression from low FLO11 transcript abundances.
Fischer
Claudia
C
Institute of Microbiology and Genetics, Georg-August-University, Göttingen, Germany.
Valerius
Oliver
O
Rupprecht
Heike
H
Dumkow
Marc
M
Krappmann
Sven
S
Braus
Gerhard H
GH
D000596:Amino Acids / Q000378:metabolism*; D019677:Artificial Gene Fusion; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D051548:Histone Acetyltransferases / Q000235:genetics; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism; D000099224:p300-CBP-Associated Factor
D000596:Amino Acids; D002868:Chromosomal Proteins, Non-Histone; D051548:Histone Acetyltransferases; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D001616:beta-Galactosidase; C110690:FLO11 protein, S cerevisiae; C501075:RSC1 protein, S cerevisiae; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor