[Preparation and identification of monoclonal antibodies against snake venom C-type lectin like protein Agkisacutacin].
OBJECTIVE
To prepare and identify the antibodies against snake venom C-type lectin like protein Agkisacutacin.


METHODS
A BALB/c mouse was immunized with Agkisacutacin and the cells were fused by standard hybridoma technique to prepare mAbs. The stability of the hybridomas secreting mAbs was detected by indirect ELISA. The nuclear type of the hybridomas was analyzed by fluorescent staining and the specificity of mAbs was detected by Western blot.


RESULTS
Three cell strains of hybridomas that could steadily secrete anti-Agkisacutacin mAb were obtained.


CONCLUSIONS
The successful preparation of anti-Agkisacutacin mAbs provides an important tool for studying the in vivo metabolism of new anti-thrombus drugs by detecting Agkisacutacin and investigating the mechanism of anti-thrombus of the drugs.
Su
Li-Jing
LJ
Lab of Cellular and Molecular Immunology, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
Xiao
Liang
L
Zhan
Tao
T
Chen
Lan
L
Dong
Yan-Qiu
YQ
Fang
Li
L
Cheng
Lian-Sheng
LS
Liu
Jing
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology* / Q000378:metabolism; D000918:Antibody Specificity; D003435:Crotalid Venoms / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D012910:Snake Venoms / Q000737:chemistry* / Q000276:immunology
D000911:Antibodies, Monoclonal; D003435:Crotalid Venoms; D012910:Snake Venoms; C401844:agkisacutacin