title: Exposure of mouse oocytes to 1,2-propanediol during slow freezing alters the proteome.
abstract: OBJECTIVE
  To examine the effect of exposure to 1,2-propanediol (PrOH) on the mammalian oocyte proteome.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  Research laboratory.
  
  
  METHODS
  Four- to five-week-old F1 (C57BL/6 x CBA/Ca) female mice.
  
  
  METHODS
  Analysis by time-of-flight mass spectrometry.
  
  
  METHODS
  Protein profiles of denuded mouse metaphase II (MII) oocytes.
  
  
  RESULTS
  Metaphase II oocytes exposed to 1.5 M PrOH for 20 minutes with or without seeding (i.e., equilibration, dehydration with or without seeding) exhibited significantly altered protein profiles in comparison to controls (i.e., 37 degrees C for 20 minutes and room temperature for 20 min). In total, 11 protein-biomarkers displayed profiles of statistically significantly lower abundance, whereas 8 protein-biomarkers were observed to be statistically significantly up-regulated. Protein profiles of MII oocytes maintained at room temperature for 20 minutes were more similar to those that were incubated for 20 minutes at 37 degrees C.
  
  
  CONCLUSIONS
  Analysis of the mouse MII oocyte at different stages of the slow-freezing protocol revealed a negative impact on oocyte physiology after PrOH exposure. In contrast, temperature changes per se (during cooling to room temperature) have limited effect on the oocyte proteome.
authors.affiliation: Colorado Center for Reproductive Medicine, Englewood, Colorado 80113, USA.
authors.forename: Mandy G
authors.initials: MG
authors.lastname: Katz-Jaffe
authors.forename: Mark G
authors.initials: MG
authors.lastname: Larman
authors.forename: Courtney B
authors.initials: CB
authors.lastname: Sheehan
authors.forename: David K
authors.initials: DK
authors.lastname: Gardner
mesh_terms: D000818:Animals; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000009:adverse effects*; D005260:Female; D005615:Freezing; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D019946:Propylene Glycol / Q000009:adverse effects*; D020543:Proteome / Q000187:drug effects*; D013997:Time Factors
chemical_list: D003451:Cryoprotective Agents; D020543:Proteome; D019946:Propylene Glycol