Endothelium dysfunction caused by acute pressure distension of human saphenous vein used for myocardial revascularization.
OBJECTIVE
To study morphofunctional alterations induced by brief pressure increases in human saphenous veins utilized in coronary artery bypass grafting.


METHODS
Saphenous veins of 20 patients undergoing coronary artery bypass grafting, were distributed into four experimental groups, control, 100 mmHg, 200 mmHg and 300 mmHg, and submitted to pressure distention over 15 seconds using Krebs solution. The evaluation included CD34 immunohistochemistry and an In vitro vascular reactivity study in organ chambers.


RESULTS
The main experimental findings were 1) From pressures of 200 mmHg there was a tendency to reduce the CD34 expression which became statistically significant at 300 mmHg; 2) There was no impairment of the contraction and relaxation as evidenced by in vitro vascular reactivity tests.


CONCLUSIONS
Although vascular reactivity impairment was not demonstrated in vitro, the CD34 expression, measured by immunohistochemistry, shows there is endothelium dysfunction at pressures of 300 mmHg.
Viaro
Fernanda
F
Function Endothelial Laboratory of the Experimental Surgery Section in the Surgery and Anatomy, Department of the Medicine School in Ribeirão Preto, University ofSão Paulo.
Carlotti
Carlos Gilberto
CG
Rodrigues
Alfredo José
AJ
Vicente
Walter Vilella de Andrade
WV
Bassetto
Solange
S
Reis
Graziela Saraiva
GS
Alves
Lafaiete
L
Evora
Paulo Roberto Barbosa
PR
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000704:Analysis of Variance; D018952:Antigens, CD34 / Q000032:analysis* / Q000378:metabolism; D001794:Blood Pressure; D016022:Case-Control Studies; D001026:Coronary Artery Bypass / Q000379:methods / Q000592:standards; D003327:Coronary Disease / Q000601:surgery; D004730:Endothelium, Vascular / Q000187:drug effects / Q000503:physiopathology*; D005260:Female; D006801:Humans; D006874:Hydrostatic Pressure; D007150:Immunohistochemistry; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology; D012501:Saphenous Vein / Q000187:drug effects / Q000502:physiology* / Q000637:transplantation; D013314:Stress, Mechanical; D013718:Tensile Strength / Q000502:physiology; D020858:Tissue and Organ Harvesting; D014655:Vascular Resistance / Q000187:drug effects / Q000502:physiology*; D014661:Vasoconstriction / Q000187:drug effects / Q000502:physiology*; D014665:Vasodilator Agents / Q000494:pharmacology
D018952:Antigens, CD34; D014665:Vasodilator Agents; D000244:Adenosine Diphosphate