Screening of carbon dioxide-requiring extreme oligotrophs from soil.
We screened soil samples for CO(2)-requiring extreme oligotrophs similar to Rhodococcus erythropolis N9T-4, which can grow on a basal salt agar medium without an organic carbon source. From 387 soil samples, three isolates were obtained and identified as Streptomyces spp. by 16S rDNA analysis. The isolates required gaseous CO(2) for growth and grew on a basal salt medium solidified by silica gel. These results suggest that such CO(2)-requiring oligotrophs occur widely in nature.
Yoshida
Nobuyuki
N
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630-0192, Japan. yoshidan@bs.naist.jp
Ohhata
Naoko
N
Yoshino
Yumi
Y
Katsuragi
Tohoru
T
Tani
Yoshiki
Y
Takagi
Hiroshi
H
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002245:Carbon Dioxide / Q000378:metabolism*; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013302:Streptomyces / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002245:Carbon Dioxide