Determination of dopamine in injections and urine by an enzyme-catalyzed fluorescence quenching method.
A new, sensitive method for determining dopamine has been established based on the principle of fluorescence quenching. A linear relationship is obtained between F(0)/F and the concentration of dopamine in the range of 0.80 -16.00 microg mL(-1). The detection limit was 0.23 microg mL(-1) and the relative stand deviation was 1.54%. The mechanism of the fluorescence quenching has been discussed. Effects of pH, foreign ions, and standing time on the determination of dopamine have been examined. This method is simple and can be used for the determination of dopamine in injections solution of dopamine. The results obtained by this method agreed with those obtained by the official method.
Wang
Huai You
HY
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, People's Republic of China. wanghuaiyou@sdnu.edu.cn
Feng
Xia Guang
XG
Zhang
Min
M
Zhao
Hu
H
D002138:Calibration; D002384:Catalysis; D004298:Dopamine / Q000737:chemistry / Q000652:urine*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D015394:Molecular Structure; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*
D007477:Ions; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D004298:Dopamine