title: [Preliminary analyses for influence of mutant PrPs with different number of octapeptide].
abstract: OBJECTIVE
  The present study was conducted to understand the effects of PrP in different octapeptide repeats on proliferation of HeLa cells.
  
  
  RESULTS
  Mutant PrPs with octapeptide repeat insertion were transiently expressed in HeLa cells and their results of MTT assay showed stronger cytotoxic effect on the proliferation of cells than wild-type PrP. Annexin V/PI assay also demonstrated that the expression of mutant PrPs was much easier to induce apoptosis than wild-type in HeLa cells. The percentage of both early and late stage apoptosis in mutant groups were significantly higher than that of wild type.
  
  
  CONCLUSIONS
  These data suggest that the expression of mutant PrPs associated with familial CJD is much easier to induce apoptosis in cultured cells than expression of wild type PrP.
authors.affiliation: Department of Prion Disease, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Han
authors.forename: Yan-Zhen
authors.initials: YZ
authors.lastname: Wan
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.forename: Lan
authors.initials: L
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Wang
authors.forename: Yin-Xia
authors.initials: YX
authors.lastname: Huang
authors.forename: Chen-Fang
authors.initials: CF
authors.lastname: Dong
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Jiang
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Dong
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D015153:Blotting, Western; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics / Q000502:physiology; D003124:Colorimetry; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D009842:Oligopeptides / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D000072002:Prion Proteins; D011328:Prions / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D014162:Transfection
chemical_list: D009842:Oligopeptides; C512656:PRNP protein, human; D000072002:Prion Proteins; D011328:Prions