YY1 repressing peroxisome proliferator-activated receptor delta promoter.
Peroxisome proliferator-activated receptors delta (PPARdelta) is a nuclear hormone receptor belonging to the steroid receptor superfamily and is molecular targets for drugs to treat hypertriglyceridemia and type 2 diabetes. Yin Yang 1 (YY1) is a transcription factor that can repress or activate transcription of the genes with which it interacts. In this report, we show that YY1 specifically interacts with the PPARdelta promoter. Overexpression of YY1 in Hela and NIH 3T3 cells repressed the activity of the PPARdelta promoter, while the PPARdelta promoter activity was enhanced when YY1 was knocked down by siRNA YY1. We also show that YY1 in nuclear extracts was able to bind the PPARdelta promoter directly. These results suggest that YY1 might be a negative regulator of PPARdelta gene expression through its direct interaction with the PPARdelta promoter.
He
Cheng-Qiang
CQ
College of Life Science, Shandong Normal University, Shandong Province 250014, China.
Ding
Nai-Zheng
NZ
Fan
Wei
W
D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D002457:Cell Extracts; D002467:Cell Nucleus / Q000378:metabolism; D002522:Chlorocebus aethiops; D005260:Female; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D009154:Mutation / Q000235:genetics; D041681:NIH 3T3 Cells; D047494:PPAR delta / Q000235:genetics*; D011247:Pregnancy; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D012097:Repressor Proteins / Q000378:metabolism*; D014158:Transcription, Genetic; D014599:Uterus / Q000166:cytology / Q000378:metabolism; D050986:YY1 Transcription Factor / Q000378:metabolism*
D002457:Cell Extracts; D047494:PPAR delta; D012097:Repressor Proteins; D050986:YY1 Transcription Factor