The stargazin C terminus encodes an intrinsic and transferable membrane sorting signal.
Activity-dependent plasticity of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptors is regulated by their auxiliary subunit, stargazin. Association with stargazin enhances alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor surface expression and modifies the receptor's biophysical properties. Fusing the cytoplasmic C terminus of stargazin to the C-terminal domains of either GluR1 or the gonadotropin-releasing hormone receptor permits efficient trafficking from the endoplasmic reticulum and sorting to the basolateral membrane without altering other properties of either receptor.
Bedoukian
Matthew A
MA
Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523-1617.
Whitesell
Jennifer D
JD
Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523-1617.
Peterson
Erik J
EJ
Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523-1617.
Clay
Colin M
CM
Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523-1617.
Partin
Kathryn M
KM
Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523-1617. Electronic address: kathy.partin@research.colostate.edu.
D000818:Animals; D015220:Calcium Channels / Q000737:chemistry*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D016764:Cell Polarity; D004285:Dogs; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006801:Humans; D021382:Protein Sorting Signals; D051381:Rats; D018091:Receptors, AMPA / Q000378:metabolism; D011967:Receptors, Gonadotropin / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D015220:Calcium Channels; D021382:Protein Sorting Signals; D018091:Receptors, AMPA; D011967:Receptors, Gonadotropin; D011993:Recombinant Fusion Proteins; C491911:Cacng2 protein, rat