title: The aldehyde/alcohol dehydrogenase (AdhE) in relation to the ethanol formation in Thermoanaerobacter ethanolicus JW200.
abstract: A bifunctional aldehyde/alcohol dehydrogenase gene (adhE) from Thermoanaerobacter ethanolicus JW200 was identified and cloned. To unambiguously characterize the activity of AdhE, the recombinant protein was purified. The purified AdhE exhibited high enzymatic activity attributed to aldehyde dehydrogenase (11.0+/-0.3U/mg) and low alcohol dehydrogenase activity (2.6+/-0.2U/mg). Analysis of adhE homologous expression in T. ethanolicus showed that AdhE affected ethanol production.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Bioresources, Nanjing Normal University, Nanjing, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
authors.forename: Guogan
authors.initials: G
authors.lastname: Wu
authors.forename: Weilan
authors.initials: W
authors.lastname: Shao
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000444:Aldehyde Dehydrogenase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D000431:Ethanol / Q000378:metabolism*; D046968:Thermoanaerobacter / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase