In vitro propagation of the microsporidian pathogen Brachiola algerae and studies of its chromosome and ribosomal DNA organization in the context of the complete genome sequencing project.
Brachiola algerae has a broad host spectrum from human to mosquitoes. The successful infection of two mosquito cell lines (Mos55: embryonic cells and Sua 4.0: hemocyte-like cells) and a human cell line (HFF) highlights the efficient adaptive capacity of this microsporidian pathogen. The molecular karyotype of this microsporidian species was determined in the context of the B. algerae genome sequencing project, showing that its haploid genome consists of 30 chromosomal-sized DNAs ranging from 160 to 2240 kbp giving an estimated genome size of 23 Mbp. A contig of 12,269 bp including the DNA sequence of the B. algerae ribosomal transcription unit has been built from initial genomic sequences and the secondary structure of the large subunit rRNA constructed. The data obtained indicate that B. algerae should be an excellent parasitic model to understand genome evolution in relation to infectious capacity.
Belkorchia
Abdel
A
Laboratoire de Biologie des Protistes, UMR CNRS 6023, Université Blaise Pascal, 24 avenue des Landais, Campus des Cézeaux, 63177 Aubière Cedex, France.
Biderre
Corinne
C
Militon
Cécile
C
Polonais
Valérie
V
Wincker
Patrick
P
Jubin
Claire
C
Delbac
Frédéric
F
Peyretaillade
Eric
E
Peyret
Pierre
P
D000818:Animals; D000852:Anopheles / Q000166:cytology / Q000469:parasitology; D000913:Antibodies, Protozoan / Q000378:metabolism; D016817:Apansporoblastina / Q000235:genetics* / Q000254:growth & development*; D001483:Base Sequence; D002460:Cell Line; D002875:Chromosomes / Q000235:genetics*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry; D023061:Gene Order; D018503:Genome, Protozoan / Q000235:genetics*; D006434:Hemocytes / Q000166:cytology / Q000469:parasitology; D006801:Humans; D051379:Mice; D016881:Microsporidiosis / Q000469:parasitology; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D054683:Ribosome Subunits, Large, Eukaryotic / Q000737:chemistry
D000913:Antibodies, Protozoan; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer