title: Mycobacterium avium subspecies paratuberculosis in Bison (Bison bison) from Northern Canada.
abstract: Nested polymerase chain reaction (PCR) using the Mycobacterium avium subspecies paratuberculosis (Map)-specific region, locus 251, was used as a screening tool for the detection of Map DNA in fecal samples from northern Canadian bison herds. Further characterization of positive samples (26/835) was performed because Map DNA was found without signs of disease. Strain typing, using PCR-Restriction endonucleas assay (REA), was limited to two samples but revealed that the samples corresponded to a cattle-related strain and a sheep-related strain. Sequencing of part of the IS1311 region from the two samples revealed a unique three base-pair region, which is only found within the northern Canadian bison isolates.
authors.affiliation: Department of Microbiology and Immunology, University of British Colombia, 6174 University Blvd., Vancouver, British Columbia V6T 1Z3, Canada.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Sibley
authors.forename: Murray R
authors.initials: MR
authors.lastname: Woodbury
authors.forename: Greg D
authors.initials: GD
authors.lastname: Appleyard
authors.forename: Brett
authors.initials: B
authors.lastname: Elkin
mesh_terms: D000818:Animals; D016164:Bison / Q000382:microbiology*; D002170:Canada / Q000453:epidemiology; D003247:Conservation of Natural Resources; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D003937:Diagnosis, Differential; D005243:Feces / Q000382:microbiology; D005260:Female; D008297:Male; D016927:Mycobacterium avium subsp. paratuberculosis / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000175:diagnosis* / Q000453:epidemiology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial