[Establishment of Caco-2 cell monolayer model and standard operation procedure for assessing intestinal absorption of chemical components of traditional Chinese medicine].
OBJECTIVE
To establish Caco-2 (a human colon adenocarcinoma cell line) cell monolayer model and the standard operation procedure for studying and assessing intestinal absorption of chemical components of traditional Chinese medicine.


METHODS
Caco-2 cell monolayer model was established and evaluated by morphology feature using scanning electron microscope, inverted microscope and transepithelial electrical resistance (TEER) assay. Additionally, the model was further tested for the activity of alkaline phosphatase and the apparent permeability (Papp) of standard compounds, i.e. propranolol and atenolol, which were the control substances for high and poor transcellular transport marker, respectively.


RESULTS
The integrality of cell monolayer, cell differentiation (reflected by expression of alkaline phosphatase and cell monolayer morphology), and the Papp value of standard compounds in the established Caco-2 cell model were satisfactory. All parameters tested were in good agreement with those reported in the literature.


CONCLUSIONS
The established Caco-2 cell model can be used to study the intestinal absorption of orally administrated chemical components of traditional Chinese medicine and their absorption mechanism.
Yang
Xiu-wei
XW
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China. xwyang@bjmu.edu.cn
Yang
Xiao-da
XD
Wang
Ying
Y
Ma
Lian
L
Zhang
Yue
Y
Yang
Xiao-gai
XG
Wang
Kui
K
D001692:Biological Transport / Q000502:physiology; D018938:Caco-2 Cells; D002463:Cell Membrane Permeability; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000493:pharmacokinetics*; D005419:Flavonoids / Q000493:pharmacokinetics; D006801:Humans; D007408:Intestinal Absorption; D008954:Models, Biological
D004365:Drugs, Chinese Herbal; D005419:Flavonoids