title: Development of a sensor prepared by entrapment of MIP particles in electrosynthesised polymer films for electrochemical detection of ephedrine.
abstract: A voltammetric sensor for (-)-ephedrine has been prepared by a novel approach based on immobilisation of an imprinted polymer for ephedrine (MIPE) in an electrosynthesised polypyrrole (PPY) film. Composite films were grown potentiostatically at 1.0 V vs. Pt (QRE) on a glassy carbon electrode using an unconventional "upside-down" (UD) geometry for the three-electrode cell. As a consequence, a high MIP loading was obtained, as revealed by SEM. The sensor response was evaluated, after overoxidation of PPY matrix, by cyclic voltammetry after pre-concentration in a buffered solution of analyte in 0.5-3 mM concentration range. An ephedrine peak at approximately 0.9 V increasing with concentration and saturating at high concentrations was evident. PPY-modified electrode showed a response, which was distinctly lower than the MIP response for the same concentration of the template. The effect of potential interferences including compounds usually found in human fluids (ascorbic acid, uric acid, urea, glucose, sorbitol, glycine, dopamine) was examined.
authors.affiliation: Laboratorio di Chimica Analitica, Dipartimento di Scienza dei Materiali, Università del Salento, Via Arnesano, Lecce, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Mazzotta
authors.forename: R A
authors.initials: RA
authors.lastname: Picca
authors.forename: C
authors.initials: C
authors.lastname: Malitesta
authors.forename: S A
authors.initials: SA
authors.lastname: Piletsky
authors.forename: E V
authors.initials: EV
authors.lastname: Piletska
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation*; D004595:Electroplating / Q000379:methods; D004809:Ephedrine / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008567:Membranes, Artificial; D008863:Microspheres; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D008567:Membranes, Artificial; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D004809:Ephedrine