title: Grass carp transforming growth factor-beta 1 (TGF-beta 1): molecular cloning, tissue distribution and immunobiological activity in teleost peripheral blood lymphocytes.
abstract: Transforming growth factor-beta 1 (TGF-beta 1) is a potent regulatory cytokine with pleiotropic effects on the immune system. To examine the role of TGF-beta 1 in fish immunity, the full-length cDNA of grass carp TGF-beta 1 was isolated from grass carp spleen. The open reading frame of grass carp TGF-beta1, 1134 bp in length, encodes a 377 amino acid protein. Tissue distribution study by RT-PCR showed TGF-beta 1 mRNA was predominantly expressed in the thymus, head kidney and spleen in grass carp tissues. Moreover, the time-course effect of TGF-beta 1 on peripheral blood lymphocyte proliferation in response to mitogens was evaluated in grass carp. Interestingly, TGF-beta1 induced PBL proliferation while it significantly blocked phytohemagglutinin- or lipopolysaccharide-stimulated PBL proliferation, and TGF-beta 1 mimicked the stimulatory effects of lipopolysaccharide on grass carp MHC I mRNA expression. These results, for the first time, strongly suggest that TGF-beta 1 plays a functional role in lymphocyte proliferation in fish.
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, People's Republic of China.
authors.forename: Mu
authors.initials: M
authors.lastname: Yang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000097:blood / Q000378:metabolism*; D049109:Cell Proliferation; D003001:Cloning, Molecular; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D010835:Phytohemagglutinins / Q000494:pharmacology; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D015395:Histocompatibility Antigens Class I; D008070:Lipopolysaccharides; D008934:Mitogens; D010835:Phytohemagglutinins; D053773:Transforming Growth Factor beta1