[Analysis of signal peptides of the secreted proteins in Ralstonia solanacearum GMI1000].
The 3,440 open reading frames (ORFs) of Ralstonia solanacearum GMI1000 were used for predicting signal peptides by comprehensive analyses with SignalP 3.0, TMHMM 2.0, TargetP 1.01, LipoP 1.0 and PSORTb, and screening based on L value. Total 186 signal peptides with conserved amino acid residues are found and among them, 134 are secretary types, 22 are RR-motif types and 30 are lipoprotein signal peptides. The length distribution of signal peptide and its domains were analyzed systemically. No type pilin-like signal peptides and bacteriocins and pheronones signal peptides are found in the database of deduced proteins encoded by the genome of R. solanacearum GMI1000.
Huang
Jun-Li
JL
College of Biological Engineering, Chongqing University, Chongqing 400030, China. jlh7603@yahoo.com.cn
Wu
Jin-Zhong
JZ
Xiao
Chong-Gang
CG
Li
Chang-Jun
CJ
Wang
Gui-Xue
GX
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D011237:Predictive Value of Tests; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology*; D043368:Ralstonia solanacearum / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D020539:Sequence Analysis, Protein
D001426:Bacterial Proteins; D021382:Protein Sorting Signals