[Immunolocalization of the antioxidant protein TPx of Echinococcus granulosus].
OBJECTIVE
To study the localization of the antioxidant protein thioredoxin peroxidase (TPx) of Echinococcus granulosus (EgTPx) in the protoscoleces (PSC) of parasite.


METHODS
The protoscoleces of E.granulosus were aspirated and pooled from sheep liver hydatid cysts. After digested by pepsin, the sedimented protoscoleces were used for paraffin sections. The localization of the antioxidant protein TPx of EgTPx was determined by using(polyclonal antibody against rEgTPx) and indirect immunofluorescence staining.


RESULTS
Indirect immunofluorescence staining analysis indicated that the rEgTPx was mainly distributed in tegument, subtegument and calcareous corpuscle cells of the protoscolex.


CONCLUSIONS
The wide distribution and large sites of EgTPx in the parasite hare been clearly determined, which will help further investigation into the biological functions and application of TPx protein.
Hou
Qiu-Lian
QL
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
Wang
Hui
H
Zhang
Zhuang-Zhi
ZZ
Cao
Wen-Yao
WY
Zhang
Fu-Chun
FC
Zhang
Wen-Bao
WB
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D048209:Echinococcus granulosus / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D054464:Peroxiredoxins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*
D000975:Antioxidants; D007106:Immune Sera; D054464:Peroxiredoxins