title: Conformational change induced by metal-ion-binding to DNA containing the artificial 1,2,4-triazole nucleoside.
abstract: A conformational switch can be induced upon the addition of transition-metal ions to oligonucleotides that contain a row of successive artificial nucleobases flanked by complementary sequences of natural nucleobases, provided that the artificial bases cannot undergo self-pairing via hydrogen bonding but only via the formation of metal-ion-mediated base pairs. Such oligonucleotides adopt a hairpin structure in the absence of transition-metal ions, yet they show a preference for the formation of a regular double helix if the appropriate metal ions are present. We report here our experimental data on the structure of the oligonucleotide d(A7X3T7) (A=adenine, T=thymine, X=1,2,4-triazole) in the absence and presence of silver(I). This study comprising temperature-dependent UV spectroscopy, CD spectroscopy, MALDI-TOF measurements, fluorescence spectroscopy, and dynamic light scattering opens up a new approach to the generation of a large variety of metal-ion sensors with the possibility of fine-tuning their sensing capabilities, depending on the artificial nucleoside that is used.
authors.affiliation: Department of Chemistry, University of Dortmund, Otto-Hahn-Strasse 6, 44227 Dortmund, Germany.
authors.forename: Dominik
authors.initials: D
authors.lastname: Böhme
authors.forename: Nicole
authors.initials: N
authors.lastname: Düpre
authors.forename: Dominik A
authors.initials: DA
authors.lastname: Megger
authors.forename: Jens
authors.initials: J
authors.lastname: Müller
mesh_terms: D000225:Adenine / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013696:Temperature; D013941:Thymine / Q000737:chemistry; D014230:Triazoles / Q000737:chemistry*
chemical_list: D009705:Nucleosides; D009841:Oligonucleotides; D014230:Triazoles; D012834:Silver; D004247:DNA; D000225:Adenine; D013941:Thymine