title: [Effects of membrane structure on apolipoprotein A-I binding to lipid].
abstract: Interaction of apolipoproteins with lipid surfaces plays crucial roles in lipoprotein metabolism and cholesterol homeostasis. In this study, to understand the detailed mechanism by which apolipoprotein (apo) A-I associates with plasma membrane and lipoprotein particles, we investigated the effects of lipid composition and surface curvature on the lipid-apoA-I interactions. ApoA-I binding to lipid particles depends on membrane structure. Fluorescence and (13)C-NMR measurements revealed that apoA-I recognizes headgroup separation (hydrated space) between phospholipid molecules and displaces water molecules from the surface when it binds. At the surface monolayers of lipoprotein particles, interpenetration of core triglyceride molecules with phospholipid molecules appears to occur to alter the surface structure. ApoA-I binding to lipid membrane induces an increase in alpha-helical structure. This alpha-helix formation generates exothermic heat and promotes apoA-I binding to lipid. However, binding of apoA-I to small emulsions exhibited endothermic heat although apoA-I increases alpha-helical content. Taken together, these observations suggest that the presence of core triglyceride modifies the highly curved emulsion surface and thereby the thermodynamics of apoA-I binding in a manner that compensates for the exothermic heat generated by alpha-helix formation.
authors.affiliation: Department of Biophysical Chemistry, Kobe Pharmaceutical University, Motoyama-kitamachi, Kobe, Japan. masatnk@kobepharma-u.ac.jp
authors.forename: Masafumi
authors.initials: M
authors.lastname: Tanaka
mesh_terms: D016632:Apolipoprotein A-I / Q000737:chemistry* / Q000378:metabolism*; D004655:Emulsions; D008563:Membrane Lipids / Q000737:chemistry* / Q000378:metabolism*; D010316:Particle Size; D011485:Protein Binding; D017433:Protein Structure, Secondary; D013816:Thermodynamics
chemical_list: D016632:Apolipoprotein A-I; D004655:Emulsions; D008563:Membrane Lipids