title: Yeast cell-surface expression of chitosanase from Paenibacillus fukuinensis.
abstract: To produce chitoorigosaccharides using chitosan, we attempted to construct Paenibacillus fukuinensis chitosanase-displaying yeast cells as a whole-cell biocatalyst through yeast cell-surface engineering. The localization of the chitosanase on the yeast cell surface was confirmed by immunofluorescence labeling of cells. The chitosanase activity of the constructed yeast was investigated by halo assay and the dinitrosalicylic acid method.
authors.affiliation: Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fukuda
authors.forename: Danya
authors.initials: D
authors.lastname: Isogawa
authors.forename: Madoka
authors.initials: M
authors.lastname: Takagi
authors.forename: Michiko
authors.initials: M
authors.lastname: Kato-Murai
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kimoto
authors.forename: Hideo
authors.initials: H
authors.lastname: Kusaoke
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ueda
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Suye
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D001709:Biotechnology / Q000379:methods*; D002384:Catalysis; D002462:Cell Membrane / Q000201:enzymology*; D003001:Cloning, Molecular; D005818:Genetic Engineering; D006026:Glycoside Hydrolases / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D009844:Oligosaccharides / Q000096:biosynthesis; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D009844:Oligosaccharides; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C019868:chitosanase