Histological mapping of biochemical changes in solid tumors by FT-IR spectral imaging.
Fourier-transform infrared (FT-IR) spectral imaging was used for analyzing biochemical changes in tumor cells. Metabolic parameters of human lung A549/8 adenocarcinoma and U87 glioma cells were compared under stress conditions in culture along with tumor progression after cell implantation onto the chick embryo chorio-allantoic membrane. In cell culture, glucose consumption and lactic acid release were higher in U87 cells. A549/8 cells were less sensitive to oxidative stress as observed through changes in fatty acyl chains. In vivo biochemical mapping of highly (U87) vs. poorly (A549/8) angiogenic tumors provided results comparable to culture models. Therefore, FT-IR imaging allows detecting subtle chemical changes in tumors, which might be useful for diagnosis.
Petibois
Cyril
C
University of Bordeaux 2, Centre National de la Recherche Scientifique, UMR 5084, 146 Rue Léo Saignat, F33076 Bordeaux, France. cyril.petibois@u-bordeaux2.fr
Drogat
Benjamin
B
Bikfalvi
Andreas
A
Déléris
Gérard
G
Moenner
Michel
M
D015687:Cell Hypoxia / Q000187:drug effects; D045744:Cell Line, Tumor; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D005947:Glucose