title: Association between heat-shock protein 70 gene polymorphisms and DNA damage in peripheral blood lymphocytes among coke-oven workers.
abstract: Hsp70 has been shown to act as a chaperone and be associated with cytoprotection against DNA damage caused by environmental stresses. However, it is unknown whether genetic variation in HSP70 plays a role in stress tolerance and cytoprotection against DNA damage. We determined the frequencies of three polymorphisms, HSP70-1 G190C, HSP70-2 G1267A, and HSP70-hom T2437C from 251 steel-plant workers exposed to coke-oven emission and 130 controls. We estimated the association between the HSP70variants/haplotypes and the levels of DNA damage in their peripheral blood lymphocytes detected by single-cell gel electrophoresis assay. Our results showed that overall coke-oven workers had higher levels of the Olive tail moment (Olive TM) (1.27+/-1.12) than that of the controls (0.56+/-0.99, P<0.001). Coke-oven workers with the HSP70-1 C/C genotype had higher levels of Olive TM (2.19+/-0.65), compared with HSP70-1 G/C and G/G carriers (Olive TM=1.34+/-1.09 and 1.14+/-1.08, respectively, P=0.022 and 0.003, respectively). However, the HSP70-2 G1267A and HSP70-hom T2437C polymorphisms were not associated with the levels of Olive TM (P=0.929 and 0.795, respectively). Haplotype analysis showed that carriers of TCG/TCG haplotype pairs had the highest levels of Olive TM among both the exposed subjects (2.04+/-0.59) and the controls (0.81+/-0.59). Our results suggest that the individuals with the homozygous HSP70-1 C/C genotype among the coke-oven workers may be susceptible to DNA damage.
authors.affiliation: Institute of Occupational Medicine and Ministry of Education Key Lab for Environment and Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: J
authors.initials: J
authors.lastname: Yuan
authors.forename: J
authors.initials: J
authors.lastname: Sun
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Liang
authors.forename: Y
authors.initials: Y
authors.lastname: Bai
authors.forename: L
authors.initials: L
authors.lastname: Guo
authors.forename: H
authors.initials: H
authors.lastname: Tan
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Su
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: R M
authors.initials: RM
authors.lastname: Tanguay
authors.forename: T
authors.initials: T
authors.lastname: Wu
mesh_terms: D003077:Coke; D020552:Comet Assay; D004249:DNA Damage; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006239:Haplotypes; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D009784:Occupational Diseases / Q000209:etiology / Q000235:genetics*; D016273:Occupational Exposure / Q000009:adverse effects / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D011110:Polymorphism, Genetic
chemical_list: D003077:Coke; D018840:HSP70 Heat-Shock Proteins; D011084:Polycyclic Aromatic Hydrocarbons