[Investigation of the variation of Cry j 2 concentration in pollen among sugi (Cryptomeria japonica d. Don) trees using a newly established extraction method].
BACKGROUND
Selection of low-allergen trees is under consideration for the reduction of Sugi (Cryptomeria japonica) pollinosis. The extraction method of Cry j 2 was established and then the variation of Cry j 2 concentrations in pollen among Sugi trees was investigated.


METHODS
The extraction efficiency of Cry j 2 was compared among buffers with different pH, ionic species and NaCl concentration. The Cry j 2 concentration in the pollen of 140 trees was measured using the most efficient buffer.


RESULTS
NaHCO3 (0.125 M) + 0.5 M NaCl buffer showed the highest Cry j 2 extraction efficiency. The mean and standard deviation of Cry j 2 concentration among 140 trees was 404 +/- 189 microg/g pollen, which was equivalent to that of Cry j 1 (435 +/- 169 microg).


CONCLUSIONS
We revealed that the variation of Cry j 2 concentrations was as large as that of Cry j 1 in the pollen among trees using the newly developed extraction method.
Goto-Fukuda
Yoko
Y
Hokkaido Regional Breeding Office, Forest Tree Breeding Center, Forestry and Forest Products Research Institute, Japan. gomama@affrc.go.jp
Yasueda
Hiroshi
H
Saito
Akemi
A
Kondo
Teiji
T
D002021:Buffers; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D032485:Cryptomeria; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D010940:Plant Proteins / Q000302:isolation & purification*; D011058:Pollen / Q000737:chemistry*; D012965:Sodium Chloride
D002021:Buffers; C065291:Cry j II protein, Cryptomeria japonica; D010940:Plant Proteins; D012965:Sodium Chloride