title: Effects of gamma irradiation on mechanical behavior and calcification of glutaraldehyde-fixed bovine pericardium.
abstract: OBJECTIVE
  To evaluate the effect of gamma irradiation on glutaraldehyde-fixed bovine pericardium.
  
  
  METHODS
  Glutaraldehyde-fixed bovine pericardium was exposed to gamma radiation (doses from 0 to 10000 Gy). Six samples from each of nine groups were evaluated by optic microscopy, and shrinking and mechanical tests and the denaturation temperature was determined. Additionally, they were subcutaneously implanted in rats and after four months they were explanted and Ca2+ levels measured by atomic absorption spectroscopy.
  
  
  RESULTS
  The Ca2+ levels were (in microg/mg): control (0 Gy) - 194.45; 50 Gy - 154.64; 100 Gy - 169.37; 200 Gy - 163.64; 500 Gy - 199.89; 1000 Gy - 184.02; 2000 Gy - 198.95; 5000 Gy - 227.95; 10000 Gy - 362.62. Gamma irradiation caused a significant effect on the biomechanical properties of the tissue.
  
  
  CONCLUSIONS
  e-fixed bovine pericardium.
authors.affiliation: Instituto de Pesquisas Energéticas e Nucleares(IPEN) - Linked to the University of São Paulo, São Paulo, SP. jabaucia@hotmail.com
authors.forename: José Augusto
authors.initials: JA
authors.lastname: Baucia
authors.forename: Ricardo Mendes
authors.initials: RM
authors.lastname: Leal Neto
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Rogero
authors.forename: Nanci do
authors.initials: Nd
authors.lastname: Nascimento
authors.forename: Carmem Aparecida Cruz
authors.initials: CA
authors.lastname: Reyes
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis / Q000528:radiation effects*; D000818:Animals; D002114:Calcinosis / Q000517:prevention & control*; D002118:Calcium / Q000032:analysis; D002417:Cattle; D003094:Collagen; D004195:Disease Models, Animal; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005404:Fixatives / Q000494:pharmacology*; D005720:Gamma Rays / Q000627:therapeutic use*; D005976:Glutaral / Q000494:pharmacology*; D010496:Pericardium / Q000528:radiation effects*; D019736:Prostheses and Implants; D051381:Rats; D013314:Stress, Mechanical
chemical_list: D005404:Fixatives; D003094:Collagen; D000469:Alkaline Phosphatase; D002118:Calcium; D005976:Glutaral