Steady state protein levels in Geobacter metallireducens grown with iron (III) citrate or nitrate as terminal electron acceptor.
Geobacter species predominate in aquatic sediments and submerged soils where organic carbon sources are oxidized with the reduction of Fe(III). The natural occurrence of Geobacter in some waste sites suggests this microorganism could be useful for bioremediation if growth and metabolic activity can be regulated. 2-DE was used to monitor the steady state protein levels of Geobacter metallireducens grown with either Fe(III) citrate or nitrate to elucidate metabolic differences in response to different terminal electron acceptors present in natural environments populated by Geobacter. Forty-six protein spots varied significantly in abundance (p<0.05) between the two growth conditions; proteins were identified by tryptic peptide mass and peptide sequence determined by MS/MS. Enzymes involved in pyruvate metabolism and the tricarboxylic acid (TCA) cycle were more abundant in cells grown with Fe(III) citrate, while proteins associated with nitrate metabolism and sensing cellular redox status along with several proteins of unknown function were more abundant in cells grown with nitrate. These results indicate a higher level of flux through the TCA cycle in the presence of Fe(III) compared to nitrate. The oxidative stress response observed in previous studies of Geobacter sulfurreducens grown with Fe(III) citrate was not seen in G. metallireducens.
Ahrendt
Angela J
AJ
Argonne National Laboratory, Biosciences Division, Argonne, IL, USA.
Tollaksen
Sandra L
SL
Lindberg
Carl
C
Zhu
Wenhong
W
Yates
John R
JR
Nevin
Kelly P
KP
Babnigg
György
G
Lovley
Derek R
DR
Giometti
Carol S
CS
D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004583:Electrons; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005290:Ferric Compounds / Q000378:metabolism / Q000494:pharmacology*; D045302:Geobacter / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D009566:Nitrates / Q000378:metabolism / Q000494:pharmacology*; D010084:Oxidation-Reduction; D040901:Proteomics; D053719:Tandem Mass Spectrometry / Q000379:methods
D001426:Bacterial Proteins; D005290:Ferric Compounds; D009566:Nitrates; C025314:ferric citrate; C025302:ferric nitrate