Solid-phase extraction of ochratoxin A from wine based on a binding hexapeptide prepared by combinatorial synthesis.
In this paper we describe the preparation of a hexapeptide library by combinatorial synthesis and the identification of a peptide with sequence Ser-Asn-Leu-His-Pro-Lys, which showed good affinity (K(eq)=3.4 x 10(4) M(-1)) towards the mycotoxin ochratoxin A (OTA). An immunoaffinity-like stationary phase supporting such a hexapeptide was used to develop a solid-phase extraction method for the quantification of OTA in wine samples at concentration levels down to 0.10 microg l(-1). Several different wine samples fortified with OTA at 2 and 4 microg l(-1) levels showed recovery of 94.7% and 98.4% at 2.0 and 4.0 microg l(-1), respectively, without any effect on the extraction efficiency of the matrix. The efficacy of this approach was successfully tested by comparison with an immunoaffinity extraction performed on commercial cartridges.
Giraudi
G
G
Dipartimento di Chimica Analitica, Università di Torino, Turin, Italy.
Ferrero
Valentina E V
VE
Anfossi
L
L
Baggiani
C
C
Giovannoli
C
C
Tozzi
C
C
D000595:Amino Acid Sequence; D020650:Combinatorial Chemistry Techniques; D009793:Ochratoxins / Q000302:isolation & purification*; D009842:Oligopeptides / Q000737:chemistry; D019151:Peptide Library; D052616:Solid Phase Extraction / Q000379:methods*; D014920:Wine / Q000032:analysis*
D009793:Ochratoxins; D009842:Oligopeptides; D019151:Peptide Library; C025589:ochratoxin A