Antioxidant vitamins and adrenocorticotrophic hormone-induced hypertension in rats.
This study examined whether the anti-oxidants ascorbic acid, alpha- or gamma-tocopherol, could modify adrenocorticotrophic hormone (ACTH)-hypertension in Sprague-Dawley rats, a model associated with increased oxidative stress. Systolic blood pressure (SBP) was measured by the tail-cuff method. After four days of ascorbic acid (AA) (200 mg/kg/day drinking) or alpha-tocopherol (500 mg/kg/d i.p. or feed), rats were co-administered ACTH (0.2 mg/kg/day s.c.) or saline for 11 days (prevention studies). In reversal studies, ACTH/saline was administered for 15 days, and from day 9, alpha- or gamma-tocopherol (20 mg/kg/day) was added. ACTH increased SBP compared to saline (p < 0.05). AA or alpha-tocopherol failed to prevent and alpha- or gamma-tocopherol failed to reverse ACTH-induced hypertension. Thus, neither vitamin C (water soluble) nor E (lipid soluble) modified ACTH-induced hypertension in the rat.
Schyvens
Christopher G
CG
High Blood Pressure Research Unit, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
Andrews
Miles C
MC
Tam
Rachel
R
Mori
Trevor A
TA
Croft
Kevin D
KD
McKenzie
Katja U S
KU
Whitworth
Judith A
JA
Zhang
Yi
Y
D000324:Adrenocorticotropic Hormone / Q000009:adverse effects; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D004195:Disease Models, Animal; D006973:Hypertension / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013599:Systole / Q000187:drug effects; D024505:Tocopherols / Q000494:pharmacology*; D014815:Vitamins / Q000494:pharmacology*
D000975:Antioxidants; D014815:Vitamins; D000324:Adrenocorticotropic Hormone; D001205:Ascorbic Acid; D024505:Tocopherols