Knockdown of human MCM10 exhibits delayed and incomplete chromosome replication.
In model organisms, MCM10 is required for forming the pre-initiation complex for initiation of chromosome replication and is involved in the elongation step. To investigate the role of MCM10 in human chromosome replication, we used small interfering RNA (siRNA) in MCM10-knockdown experiments and found that knockdown accumulated S and G2 phase cells. The chromosome replication of MCM10-knockdown cells was slowed during early and mid S phases, although Cdc45, Polalpha, and PCNA proteins were loaded onto the chromatin, and was aberrant during late S phase. Our results indicate that MCM10 is essential for the efficient elongation step of chromosome replication.
Park
Jong Hoon
JH
Department of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
Bang
Sung Woong
SW
Jeon
Yesu
Y
Kang
Sukhyun
S
Hwang
Deog Su
DS
D018797:Cell Cycle Proteins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D002877:Chromosomes, Human / Q000235:genetics*; D004256:DNA Polymerase I / Q000032:analysis / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects / Q000235:genetics*; D016195:G2 Phase / Q000187:drug effects / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D064110:Minichromosome Maintenance Proteins; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology; D016196:S Phase / Q000187:drug effects / Q000235:genetics
C113571:CDC45 protein, human; D018797:Cell Cycle Proteins; D002843:Chromatin; C489613:MCM10 protein, human; D018809:Proliferating Cell Nuclear Antigen; D034741:RNA, Small Interfering; D004256:DNA Polymerase I; D064110:Minichromosome Maintenance Proteins