title: Gene expression profiling for the prediction of lymph node metastasis in patients with cervical cancer.
abstract: We investigated whether gene expression profiling of primary cervical tumor tissue could be used to predict lymph node (LN) metastasis and compared this with conventional magnetic resonance imaging. We obtained 43 primary cervical cancer samples (16 with LN metastasis and 27 without LN metastasis) for microarray analysis. A prediction model for LN metastasis from the training set was developed by support vector machine methods using a 10-fold cross-validation. The 'LN prediction model' derived from the signature of 156 distinctive genes (P < 0.01) had a prediction accuracy of 77%. Correlation between mRNA expressions measured by microarray and semiquantitative reverse transcription-polymerase chain reaction was ascertained in four (RBM8A, SDHB, SERPINB13, and gamma-interferon) out of 10 genes. Magnetic resonance imaging showed accuracy (69%) for the prediction of LN metastasis. These results suggest that gene expression profiling allows reliable prediction of LN metastasis in cervical cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Tae-Joong
authors.initials: TJ
authors.lastname: Kim
authors.forename: Jung-Joo
authors.initials: JJ
authors.lastname: Choi
authors.forename: Woo Young
authors.initials: WY
authors.lastname: Kim
authors.forename: Chel Hun
authors.initials: CH
authors.lastname: Choi
authors.forename: Jeong-Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Duk-Soo
authors.initials: DS
authors.lastname: Bae
authors.forename: Dae-Soon
authors.initials: DS
authors.lastname: Son
authors.forename: Jhingook
authors.initials: J
authors.lastname: Kim
authors.forename: Byung Kwan
authors.initials: BK
authors.lastname: Park
authors.forename: Geunghwan
authors.initials: G
authors.lastname: Ahn
authors.forename: Eun Yoon
authors.initials: EY
authors.lastname: Cho
authors.forename: Byoung-Gie
authors.initials: BG
authors.lastname: Kim
mesh_terms: D001706:Biopsy; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D008207:Lymphatic Metastasis; D008279:Magnetic Resonance Imaging; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011237:Predictive Value of Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*
chemical_list: D007371:Interferon-gamma