title: Expression of a lipid transfer protein in Escherichia coli and its phosphorylation by a membrane-bound calcium-dependent protein kinase.
abstract: Ha-AP10 is a basic antifungal peptide from sunflower seeds (Helianthus annuus antifungal peptide of 10 kDa) belonging to the family of plant lipid transfer proteins. We report here its expression in E. coli [Glutathione S-transferase (GST) system] and its phosphorylation by endogenous membrane-bound calcium-dependent protein kinases.
authors.affiliation: Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Casilla de Correo 1245, Mar del Plata, Argentina. marimart@mdp.edu.ar
authors.forename: M L
authors.initials: ML
authors.lastname: Martin
authors.forename: E Espinosa
authors.initials: EE
authors.lastname: Vidal
authors.forename: L
authors.initials: L
authors.lastname: de la Canal
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000378:metabolism; D052179:Antigens, Plant / Q000096:biosynthesis; D002352:Carrier Proteins / Q000096:biosynthesis*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis*; D011494:Protein Kinases / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D017871:Calcium-Calmodulin-Dependent Protein Kinases
chemical_list: D000935:Antifungal Agents; D052179:Antigens, Plant; D002352:Carrier Proteins; D010940:Plant Proteins; D011494:Protein Kinases; D011993:Recombinant Fusion Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C500581:Ha-AP10 protein, Helianthus annuus; C511920:lipid transfer proteins, plant