Hb Kenya among Luo adults and young children in malaria holoendemic Western Kenya: screened by high performance liquid chromatography and confirmed by polymerase chain reaction.
Hb Kenya, a fusion hemoglobin (Hb) resulting from a crossover between the (A)gamma- and beta-globin genes, is accompanied by an increased level of fetal Hb (Hb F) in adult life. This study describes the use of cation exchange high performance liquid chromatography (HPLC) in the identification of Hb Kenya and of a polymerase chain reaction (PCR) method for confirmatory diagnosis. Data came from 584 children and 406 adults who were screened for eligibility for malaria vaccine trials at Kombewa, Western Kenya. Sixteen subjects (13 children and three adults) had elevated Hb F (5.0-28.4%; normal <5.0%). Of these, 11 had an apparent markedly elevated Hb A(2) (9.2-22.9%) and were confirmed by gap-PCR as having the 22.7 kb deletion characteristic of Hb Kenya. Of the five cases with elevated Hb F but normal A(2), none had Hb Kenya. We propose that in this population, the finding by cation exchange HPLC of an elevated Hb F (>9.0%) and of an apparently increased Hb A(2) (>9.2%), may suggest the presence of Hb Kenya. However, given the inability of differentiating Hb Kenya from a truly elevated Hb A(2) by routine cation exchange HPLC, it is imperative to confirm the Hb Kenya mutation by gap-PCR.
Kifude
Carolyne M
CM
Walter Reed Project, Kenya Medical Research Institute, Kisumu, Kenya.
Polhemus
Mark E
ME
Heppner
D Gray
DG
Withers
Mark R
MR
Ogutu
Bernhards R
BR
Waitumbi
John N
JN
D000293:Adolescent; D000328:Adult; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D015331:Cohort Studies; D019353:Endemic Diseases; D005260:Female; D005319:Fetal Hemoglobin / Q000032:analysis*; D006443:Hemoglobin A2 / Q000032:analysis*; D006453:Hemoglobinopathies / Q000175:diagnosis*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D007223:Infant; D007630:Kenya / Q000453:epidemiology; D008288:Malaria / Q000453:epidemiology*; D008297:Male; D016133:Polymerase Chain Reaction
D006455:Hemoglobins, Abnormal; C040843:hemoglobin Kenya; C049102:hemoglobin A2'; D006443:Hemoglobin A2; D005319:Fetal Hemoglobin