title: Dual role of melanoma cell adhesion molecule (MCAM)/CD146 in lymphocyte endothelium interaction: MCAM/CD146 promotes rolling via microvilli induction in lymphocyte and is an endothelial adhesion receptor.
abstract: The melanoma cell adhesion molecule (MCAM)/CD146 is expressed as two isoforms differing by their cytoplasmic domain (MCAM long (MCAM-l) and MCAM short (MCAM-s)). MCAM being expressed by endothelial cells and activated T cells, we analyzed its involvement in lymphocyte trafficking. The NK cell line NKL1 was transfected by MCAM isoforms and submitted to adhesion on both the endothelial cell monolayer and recombinant molecules under shear stress. MCAM-l transfection reduced rolling velocity and increased NKL1 adhesion on the endothelial cell monolayer and VCAM-1. Scanning electron microscopy revealed that MCAM-l induced microvilli formation and extension. In contrast, MCAM short or mock transfection had no effect on adhesion of NKL1 cells and microvilli formation. As shown by mutagenesis, serine 32 of the MCAM-l cytoplasmic tail, belonging to a putative protein kinase C phosphorylation site, was necessary for MCAM-l-actin cytoskeleton interaction and microvilli induction. Accordingly, chelerythrine chloride, a protein kinase C inhibitor, abolished MCAM-l-induced microvilli and rolling of MCAM-l-transfected NKL1 cells. Inhibition of adhesion under shear stress by anti-MCAM Abs suggested that both lymphoid MCAM-l and endothelial MCAM were also directly involved in lymphocyte endothelium interaction. MCAM-l-transfected NKL1 and activated CD4 T cells adhered to rMCAM under shear stress whereas anti-MCAM Ab treatment inhibited this process. Taken together, these data establish that MCAM is involved in the initial steps of lymphocyte endothelium interaction. By promoting the rolling on the inflammation marker VCAM-1 via microvilli induction and displaying adhesion receptor activity involving possible homophilic MCAM-l-MCAM-l interactions, MCAM might be involved in the recruitment of activated T cells to inflammation sites.
authors.affiliation: Université Pierre et Marie Curie-Paris 6, Unité Mixte de Recherche 7622, Centre National de la Recherche Scientifique (CNRS), Paris, France.
authors.forename: Borhane
authors.initials: B
authors.lastname: Guezguez
authors.forename: Pascale
authors.initials: P
authors.lastname: Vigneron
authors.forename: Nathalie
authors.initials: N
authors.lastname: Lamerant
authors.forename: Claudine
authors.initials: C
authors.lastname: Kieda
authors.forename: Thierry
authors.initials: T
authors.lastname: Jaffredo
authors.forename: Dominique
authors.initials: D
authors.lastname: Dunon
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000276:immunology*; D051929:CD146 Antigen / Q000235:genetics / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D002448:Cell Adhesion / Q000235:genetics / Q000276:immunology; D002450:Cell Communication / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D002645:Chickens; D003599:Cytoskeleton / Q000276:immunology / Q000648:ultrastructure; D042783:Endothelial Cells / Q000276:immunology* / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000276:immunology / Q000473:pathology; D007694:Killer Cells, Natural / Q000276:immunology* / Q000648:ultrastructure; D036904:Leukocyte Rolling / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000276:immunology / Q000648:ultrastructure; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology; D033081:Shear Strength; D019010:Vascular Cell Adhesion Molecule-1 / Q000276:immunology
chemical_list: D030161:Avian Proteins; D051929:CD146 Antigen; C497902:MCAM protein, human; D020033:Protein Isoforms; D019010:Vascular Cell Adhesion Molecule-1; C086935:gicerin protein, Gallus gallus