title: Secretory IgAs from human milk with affinity to mammalian DNA are capable of hydrolyzing ribosomal RNA.
abstract: It was found that milk of clinical healthy women contains sIgA possessing high affinity for the mammalian thymus DNA and DNA-hydrolyzing activity (sIgA-abzymes). Here we present data that such sIgA-abzymes, purified by sequential chromatography on DEAE-fractogel, heparin-sepharose, DNA-cellulose and followed by gel-filtration, are also able to hydrolyse total RNA from E. coli better than plasmid DNA. Besides, such sIgA-abzymes effectively cleaved 18S and 28S ribosomal RNA isolated from human A549 cells. It is noteworthy that the nuclease activity of sIgA-abzymes was significantly inhibited by ATP, while dATP had no effect on it. A potential role of the ribonuclease activity of sIgA-abzymes present in human milk is discussed.
authors.affiliation: Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv. kit@cellbiol.lviv.ua
authors.forename: Y Y
authors.initials: YY
authors.lastname: Kit
authors.forename: E V
authors.initials: EV
authors.lastname: Kuligina
authors.forename: V A
authors.initials: VA
authors.lastname: Richter
authors.forename: R S
authors.initials: RS
authors.lastname: Stoika
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017151:Antibodies, Catalytic / Q000302:isolation & purification / Q000494:pharmacology*; D000915:Antibody Affinity; D002384:Catalysis; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000302:isolation & purification / Q000494:pharmacology*; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D006801:Humans; D006868:Hydrolysis; D007071:Immunoglobulin A, Secretory / Q000302:isolation & purification / Q000494:pharmacology*; D008895:Milk, Human / Q000737:chemistry* / Q000276:immunology; D012329:RNA, Bacterial / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*
chemical_list: D017151:Antibodies, Catalytic; D007071:Immunoglobulin A, Secretory; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D000255:Adenosine Triphosphate; D004247:DNA; C427203:calf thymus DNA; D004720:Endonucleases