Hypoxia regulation of facilitated glucose transporter-1 and glucose transporter-3 in mouse chondrocytes mediated by HIF-1alpha.
OBJECTIVE
Chondrocytes reside in a hypoxic environment; they utilize glucose as the main energy source. Facilitated glucose transport represents the first rate-limiting step in glucose metabolism.


RESULTS
Here we demonstrated that hypoxia induced an elevated expression level of GLUT-1 and -3 in chondrocytes. Also elevated was hypoxia inducible factor-1alpha (HIF-1alpha) protein level. On the contrary, deletion of HIF-1alpha led to complete loss of the hypoxia-induced gene expression of GLUT-1 and 3.


CONCLUSIONS
These results suggested that chondrocytes had the capacity to detect and respond to low oxygen availability with changes in expression of oxygen-regulated genes, and that hypoxia regulation of facilitated GLUT-1 and -3 was mediated by HIF-1alpha.
Ren
Bu-Fang
BF
Shanghai Institute of Traumatology & Orthopaedics and Department of Orthopaedics, Ruijin Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200025, China.
Deng
Lian-Fu
LF
Wang
Jun
J
Zhu
Ya-Ping
YP
Wei
Li
L
Zhou
Qi
Q
D053674:Aggrecans / Q000378:metabolism; D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003035:Cobalt / Q000494:pharmacology; D024043:Collagen Type II / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D051274:Glucose Transporter Type 3 / Q000378:metabolism*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism
D053674:Aggrecans; D024043:Collagen Type II; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; C497443:Hif1a protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; C495678:Slc2a1 protein, mouse; C495689:Slc2a3 protein, mouse; D003035:Cobalt; C018021:cobaltous chloride; D005947:Glucose; D010100:Oxygen