A novel medium devoid of ruminant peptone for high yield growth of Mycoplasma ovipneumoniae.
Mycoplasma ovipneumoniae is considered an emerging veterinary pathogen causing pneumonia in sheep and goats worldwide. Currently it has not been possible to define a growth medium that yields the maximum growth of M. ovipneumoniae within a short incubation period. Growth yields of M. ovipneumoniae in Eaton's medium are variable and not as consistently high as those seen with other Mycoplasma spp. This study investigated the ability of different M. ovipneumoniae field strains to grow in various media formulations, where PPLO broth was replaced by a vegetable protein source, and comparisons were made in terms of strain viability in Eaton's medium. Studies were also conducted to determine the optimal carbohydrate source for use in the M. ovipneumoniae medium. Generally, it was found that different strains showed good growth in all media tested, with growth yields at 24h in TSB-1 medium higher than those observed with Eaton's medium. Growth yields reached 10(8) to 10(9)cfu ml(-1) within 24h for particular field strains, with all strains achieving this growth level within 48-72h.
Patel
Hiren
H
School of Life Sciences, Kingston University, Penrhyn Road, Kingston-Upon-Thames, Surrey KT1 2EE, UK.
Mackintosh
David
D
Ayling
Roger D
RD
Nicholas
Robin A J
RA
Fielder
Mark D
MD
D000818:Animals; D015169:Colony Count, Microbial / Q000379:methods / Q000662:veterinary*; D003470:Culture Media / Q000737:chemistry*; D015511:Goat Diseases / Q000175:diagnosis* / Q000382:microbiology; D006041:Goats; D007700:Kinetics; D045802:Mycoplasma ovipneumoniae / Q000254:growth & development* / Q000302:isolation & purification; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis* / Q000382:microbiology
D003470:Culture Media