[Processing method and HPLC fingerprint of charred radix Scutellariae].
OBJECTIVE
To investigate the process and HPLC fingerprint of Charred Radix Scutellariae, and lay a foundation of Charred Radix Scutellariae quanlity control mode.


METHODS
Select HPLC-UV fingerprint. Chromatogram condition: Hypersil C18 column (5.0 mm x 200 mm, 5 microm), mixtures of methanol, 0.4% phosphoric acid and acetonitrile as mobile phase in a gradient mode. Flow rate: 1.0 mL x min; Detection wavelength was set at 277 nm.


RESULTS
There were no evident differences among Charred Radix Scutellariae that were normatively manufactured and processed.


CONCLUSIONS
This test proves the process is feasible, this method recurs well and can be used to provide scieitific basis for the normative process and quanlity control mode of Charred Radix Scutellariae.
Yang
Yun
Y
Henan College of Traditional Chinese Medicine, Henan, Zhengzhou 450008, China. yyun@china.com.cn
Wen
Yong-ju
YJ
Wang
Jun-min
JM
Tian
Run-tao
RT
D002606:Charcoal / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D044950:Flavanones / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D006358:Hot Temperature; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D031364:Scutellaria baicalensis / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*
D044950:Flavanones; D005419:Flavonoids; D002606:Charcoal; C038044:baicalin; C006680:baicalein