House dust mite induces expression of intercellular adhesion molecule-1 in EoL-1 human eosinophilic leukemic cells.
The house dust mite (HDM) is considered to be the most common indoor allergen associated with bronchial asthma. In this study, we investigated whether crude extract of the HDM Dermatophagoides farinae could activate human eosinophilic leukemic cells (EoL-1) to induce upregulation of cell-surface adhesion molecules. When EoL-1 cells were incubated with D. farinae extract, expression of intercellular adhesion molecule-1 (ICAM-1) significantly increased on the cell surfaces compared to cells incubated with medium alone. In contrast, surface expression of CD11b and CD49d in EoL-1 cells was not affected by D. farinae extract. In addition, pretreatment of cells with NF-kappaB inhibitor (MG-132) or JNK inhibitor (SP600125) significantly inhibited ICAM-1 expression promoted by HDM extract. However, neither p38 MAP kinase inhibitor nor MEK inhibitor prevented HDM-induced ICAM-1 expression in EoL-1 cells. These results suggest that crude extract of D. farinae induces ICAM-1 expression in EoL-1 cells through signaling pathways involving both NF-kappaB and JNK.
Kwon
Byoung Chul
BC
Department of Pediatrics and Institute of Allergy, Biomolecule Secretion Research Center, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
Sohn
Myung Hyun
MH
Kim
Kyung Won
KW
Kim
Eun Soo
ES
Kim
Kyu-Earn
KE
Shin
Myeong Heon
MH
D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D039481:CD11b Antigen / Q000096:biosynthesis; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D004804:Eosinophils / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods; D005786:Gene Expression Regulation; D006801:Humans; D039441:Integrin alpha4 / Q000096:biosynthesis; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007938:Leukemia / Q000378:metabolism*; D007976:Leupeptins / Q000494:pharmacology; D048055:Mitogen-Activated Protein Kinase 8 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D039981:Pyroglyphidae; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D000873:Anthracenes; D039481:CD11b Antigen; D007976:Leupeptins; D016328:NF-kappa B; D018799:Intercellular Adhesion Molecule-1; D039441:Integrin alpha4; C432165:pyrazolanthrone; D048055:Mitogen-Activated Protein Kinase 8; D048051:p38 Mitogen-Activated Protein Kinases; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde