Adipose differentiation of bone marrow-derived mesenchymal stem cells using Pluronic F-127 hydrogel in vitro.
Due to increasing clinical demand for adipose tissue, a suitable scaffold for engineering adipose tissue constructs is needed. In this study, we have developed a three-dimensional (3-D) culture system using bone marrow-derived mesenchymal stem cells (BM-MSC) and a Pluronic F-127 hydrogel scaffold as a step towards the in vitro tissue engineering of fat. BM-MSC were dispersed into a Pluronic F-127 hydrogel with or without type I collagen added. The adipogenic differentiation of the BM-MSC was assessed by cellular morphology and further confirmed by Oil Red O staining. The BM-MSC differentiated into adipocytes in Pluronic F-127 in the presence of adipogenic stimuli over a period of 2 weeks, with some differentiation present even in absence of such stimuli. The addition of type I collagen to the Pluronic F-127 caused the BM-MSC to aggregate into clumps, thereby generating an uneven adipogenic response, which was not desirable.
Vashi
Aditya V
AV
Bernard O'Brien Institute of Microsurgery, St. Vincent's Hospital, Fitzroy, Victoria 3065, Australia. aditya.vashi@svhm.org.au
Keramidaris
Efthimia
E
Abberton
Keren M
KM
Morrison
Wayne A
WA
Wilson
Jeremy L
JL
O'Connor
Andrea J
AJ
Cooper-White
Justin J
JJ
Thompson
Erik W
EW
D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D020442:Poloxamer / Q000494:pharmacology*; D051381:Rats
D020442:Poloxamer; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate