Optimal design and validation of antiviral siRNA for targeting HIV-1.
We propose rational designing of antiviral short-interfering RNA (siRNA) targeting highly divergent HIV-1. In this study, conserved regions within HIV-1 genomes were identified through an exhaustive computational analysis, and the functionality of siRNAs targeting the highest possible conserved regions was validated. We present several promising antiviral siRNA candidates that effectively inhibited multiple subtypes of HIV-1 by targeting the best conserved regions in pandemic HIV-1 group M strains.
Naito
Yuki
Y
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. y-naito@RNAi.jp
Nohtomi
Kyoko
K
Onogi
Toshinari
T
Uenishi
Rie
R
Ui-Tei
Kumiko
K
Saigo
Kaoru
K
Takebe
Yutaka
Y
D001483:Base Sequence; D017124:Conserved Sequence; D030541:Databases, Genetic; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics
D034741:RNA, Small Interfering; D012367:RNA, Viral