title: [Analysis of sperm chromosome in patients with chromosome balanced translocation by fluorescence in-situ hybridization].
abstract: OBJECTIVE
  To investigate the chromosome segregation patterns in patients with chromosome balanced translocation in spermatogenesis and to find out different proportions of abnormal sperm generated by different patterns.
  
  
  METHODS
  Triple color fluorescence in-situ hybridization (FISH) was used to determine the correlative chromosome constitution of spermatozoa from 4 patients with chromosome anomalies, Case 1 with 46, XY, t(9;11)(q22;q21), Case 2 with 46, XY, t(11;22) (q23;q11), Case 3 with 45, XY, t(13q;15q) and Case 4 with 45, XY, t(13q;14q). The numbers of spermatozoa generated by different chromosome segregation patterns were counted by FISH, and normal sperm from the males with normal chromosome was used as control.
  
  
  RESULTS
  The rates of abnormal sperm in the 4 cases were 50.86%, 58.33%, 13.00% and 22.82% respectively, evidently higher than their corresponding controls (0.85%, 1.63%, 1.60% and 1.37%).
  
  
  CONCLUSIONS
  To detect sperm chromosome in patients with chromosome balanced translocation by FISH can provide valuable information for predicting the risk of chromosome anomalies in the embryo preimplantation genetic diagnosis.
authors.affiliation: IVF Center of Shenzhen Zhongshan Urological Hospital, Shenzhen, Guangdong 518001, China. dearivf@sina.com
authors.forename: Yong
authors.initials: Y
authors.lastname: Zeng
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Hu
authors.forename: Qi
authors.initials: Q
authors.lastname: Lin
authors.forename: Cheng
authors.initials: C
authors.lastname: Song
mesh_terms: D000328:Adult; D025063:Chromosome Disorders / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D008297:Male; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*; D014178:Translocation, Genetic