Quantitation of B. Pseudomallei in clinical samples.
We undertook a prospective study to quantitate Burkholderia pseudomallei in blood, urine, respiratory secretions, and pus [corrected] obtained from 414 patients with melioidosis. The median was count 1.1, 1.5 x 10(4), 1.1 x 10(5), and 1.1 x 10(7) CFU/mL in these sample types, respectively. This provides important insights into the likely feasibility of future studies such as expression microarray analysis using clinical material.
Wuthiekanun
Vanaporn
V
Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Limmathurotsakul
Direk
D
Wongsuvan
Gumphol
G
Chierakul
Wirongrong
W
Teerawattanasook
Nittaya
N
Teparrukkul
Prapit
P
Day
Nicholas P
NP
Peacock
Sharon J
SJ
D000293:Adolescent; D000328:Adult; D001431:Bacteriological Techniques; D016957:Burkholderia pseudomallei / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D008554:Melioidosis / Q000097:blood / Q000382:microbiology* / Q000652:urine; D013183:Sputum / Q000382:microbiology; D013492:Suppuration / Q000382:microbiology