Tyrosinase is not detected in human catecholaminergic neurons by immunohistochemistry and Western blot analysis.
Catecholaminergic neurons of the primate substantia nigra (SN) pars compacta (SNc) and the locus coeruleus contain neuromelanin (NM) granules as characteristic structures underlying the pigmentation of these brain areas. Due to a phylogenetic appearance NM granules are absent in the rodent brain, but gradually become present in primates until they reach a maximal expression in humans. Although a possible mechanism of pigment formation may be autoxidation of the NM precursors dopamine or noradrenalin, several groups have suggested an enzymatic formation of NM mediated by tyrosinase or a related enzyme. Since tyrosinase mRNA is suggested to be expressed in the SN of mice and humans, we reinvestigated the expression of tyrosinase in the human SNc and the locus coeruleus at the protein level by immunohistochemistry and Western blot analysis, but could not detect tyrosinase in these brain regions.
Tribl
F
F
Laboratory of Clinical Neurochemistry, Clinic and Policlinic for Psychiatry and Psychotherapy, Bayerische Julius-Maximilians-Universität Würzburg, Würzburg, Germany. florian.tribl@rub.de
Arzberger
T
T
Riederer
P
P
Gerlach
M
M
D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004298:Dopamine / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007124:Immunoenzyme Techniques; D008125:Locus Coeruleus / Q000473:pathology*; D008543:Melanins / Q000378:metabolism*; D008546:Melanoma, Experimental; D051379:Mice; D014442:Monophenol Monooxygenase / Q000235:genetics*; D009474:Neurons / Q000473:pathology*; D009638:Norepinephrine / Q000378:metabolism*; D010084:Oxidation-Reduction; D013378:Substantia Nigra / Q000473:pathology*
D008543:Melanins; C014121:neuromelanin; D014442:Monophenol Monooxygenase; D004298:Dopamine; D009638:Norepinephrine