title: pH sensing in living cells using fluorescent microspheres.
abstract: Intracellular pH in living cells is measured in real time at the single cell level using fluorescently covalently loaded microspheres as efficient carrier systems and stable sensors. The use of these sensors immobilized covalently onto polymeric particles allows analysis of intracellular pH flux over long period of time and eliminates the disadvantages such as dilution within the cell, elimination via leakage or compartmentalization.
authors.affiliation: Chemical Biology Section, School of Chemistry, University of Edinburgh, Joseph Black Building, West Mains Road, EH9 3JJ Edinburgh, United Kingdom.
authors.forename: Mark
authors.initials: M
authors.lastname: Bradley
authors.forename: Lois
authors.initials: L
authors.lastname: Alexander
authors.forename: Karen
authors.initials: K
authors.lastname: Duncan
authors.forename: Mourad
authors.initials: M
authors.lastname: Chennaoui
authors.forename: Anita C
authors.initials: AC
authors.lastname: Jones
authors.forename: Rosario M
authors.initials: RM
authors.lastname: Sánchez-Martín
mesh_terms: D002460:Cell Line; D005434:Flow Cytometry / Q000379:methods*; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence / Q000379:methods*; D008863:Microspheres; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D019793:Fluorescein