Dynamic changes in parameters of redox balance after mild heat stress in aged laying hens (Gallus gallus domesticus).
In order to evaluate the metabolic responses of laying hens induced by high temperature at later laying stage, nine 60-wk-old laying hens (Gallus gallus domesticus) were employed in the present study. The hens were exposed to 32 degrees C for 21 d and blood samples were obtained before and at 1, 7, 14 and 21 d of heat exposure. The reactive oxygen species (ROS) formed in blood during heat exposure were estimated by the ex vivo spin-trapping method. Body temperature and plasma concentrations of glucose, urate, creatine kinase (CK), triiodothyronine (T(3)), thyroxine (T(4)), corticosterone (CORT), thiobarbituric acid reacting substances (TBARS), ferric/reducing antioxidant power (FRAP) and superoxide dismutase (SOD) activity were measured. Plasma levels of glucose, CK and CORT were not significantly influenced by heat exposure at any time point. The circulating concentrations of T(3) were decreased while plasma T(4) levels changed in the opposite way. The formation of ROS was significantly augmented by heat exposure in laying hens though the body temperature was not significantly altered. The enhanced enzymatic and non-enzymatic antioxidant systems acted in concert to alleviate the heat stress evoked oxidative damage.
Lin
H
H
Department of Animal Science, Shandong Agricultural University, Shandong 271018, PR China.
De Vos
D
D
Decuypere
E
E
Buyse
J
J
D000208:Acute Disease; D000367:Age Factors; D000375:Aging / Q000097:blood / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D001831:Body Temperature; D002645:Chickens; D002908:Chronic Disease; D003345:Corticosterone / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D005260:Female; D018882:Heat Stress Disorders / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D010058:Oviposition; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013974:Thyroxine / Q000097:blood; D013997:Time Factors; D014284:Triiodothyronine / Q000097:blood; D014527:Uric Acid / Q000097:blood
D000975:Antioxidants; D015415:Biomarkers; D001786:Blood Glucose; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D014284:Triiodothyronine; D014527:Uric Acid; D013482:Superoxide Dismutase; D003402:Creatine Kinase; D013974:Thyroxine; D003345:Corticosterone