[Proteomic analysis on portal vein tumor thrombus-associated proteins for hepatocellular carcinoma].
OBJECTIVE
A comparative proteomic approach was used to analyze proteins relevant to portal vein tumor thrombus forming.


METHODS
proteins extracted from five pairs of matched primary tumor/tumor thrombus samples in the same patient were separated by two-dimensional gel electrophoresis (2-DE). Selected proteins exhibiting statistically significant alternations were identified by mass spectrometry. Western blotting was further performed to examine the expression of the candidate proteins.


RESULTS
There were 20 significant proteins were identified in total, Among the 20 spots, 12 proteins were up-regulated proteins in primary tumor tissue, including Galectin-1, HMGBI, peroxiredoxin 1, Cyclophilin B, PCNA. whereas 8 were up-regulated proteins in tumor thrombus samples, including Annexin V, Triosephosphate Isomerase. Western blotting Confirmed the difference of Annexin V on protein level.


CONCLUSIONS
There are many proteins associated with the formation of PVTT in HCC. The overexpression of Annexin V may serve as a biomarker for early detection and therapeutic targets to HCC with PVTT.
Guo
Wei-Xing
WX
Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University, Shanghai 200438, China.
Man
Xiao-Bo
XB
Yuan
Hai-Xin
HX
Shi
Jie
J
Xue
Jie
J
Wu
Meng-Chao
MC
Cheng
Shu-Qun
SQ
D000328:Adult; D017304:Annexin A5 / Q000032:analysis; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D021983:Cyclophilins / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D037483:Galectin 1 / Q000032:analysis; D024002:HMGB Proteins / Q000032:analysis; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000378:metabolism; D019696:Peptidylprolyl Isomerase / Q000032:analysis; D054464:Peroxiredoxins / Q000032:analysis; D011169:Portal Vein / Q000378:metabolism* / Q000473:pathology; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D040901:Proteomics / Q000379:methods*
D017304:Annexin A5; D037483:Galectin 1; D024002:HMGB Proteins; D018809:Proliferating Cell Nuclear Antigen; C081786:cyclophilin B; C515940:PRDX1 protein, human; D054464:Peroxiredoxins; D021983:Cyclophilins; D019696:Peptidylprolyl Isomerase