Formation of N-ethylmaleimide (NEM)-glutathione conjugate and N-ethylmaleamic acid revealed by mass spectral characterization of intracellular and extracellular microbial metabolites of NEM.
The extracellular and intracellular metabolites formed upon exposure of activated sludge microorganisms to a sublethal concentration of N-ethylmaleimide were monitored by liquid chromatography with ion trap mass spectrometry. The metabolite N-ethylsuccinimido-S-glutathione (m/z 433) was converted rapidly to N-(2-oxoethyl)-2,2-(propionylamino)propanamide (m/z 187) and N-ethylmaleamic acid (m/z 144).
Mojica
Elmer-Rico E
ER
Department of Chemistry, Natural Sciences Complex, University at Buffalo, The State University of New York, Buffalo, NY 14260-3000, USA.
Kim
Sungpyo
S
Aga
Diana S
DS
D001711:Biotransformation; D002853:Chromatography, Liquid; D003470:Culture Media / Q000737:chemistry*; D003600:Cytosol / Q000737:chemistry*; D005033:Ethylmaleimide / Q000378:metabolism*; D008298:Maleates / Q000378:metabolism*; D008301:Maleimides / Q000378:metabolism*; D013058:Mass Spectrometry; D012722:Sewage / Q000382:microbiology
D003470:Culture Media; D008298:Maleates; D008301:Maleimides; C526533:N-ethylmaleamic acid; D012722:Sewage; C044107:glutathione maleimide; D005033:Ethylmaleimide