Serum prolidase and IGF-1 as non-invasive markers of hepatic fibrosis during four different periods after bile-duct ligation in rats.
OBJECTIVE
Our aim was to study the correlation of serum prolidase and insulin like growth factor-1 to liver collagen and assess their utility as markers of fibrosis during four different periods of hepatic injury and fibrosis after bile-duct ligation in rats.


METHODS
Forty-eight Wistar albino rats were included in the study and divided into six groups. Seven rats served as the control group (Control), while seven rats had a sham operation (Sham group). Thirty-four rats underwent bile-duct ligation. Bile-duct ligated (BDL) animals were sacrificed at the end of the first week (Group 1; n = 8), second week (Group 2; n = 8), third week (Group 3; n = 9), or fourth week (Group 4; n = 9) after BDL. Liver collagen, liver prolidase, and serum prolidase and IGF-I, were determined.


RESULTS
There was a positive correlation between liver collagen and serum prolidase (r(s): 0.843, P < 0.001) levels and a negative correlation among liver collagen and serum IGF-1 levels (r(s): -0.667, P < 0.001). The peak levels of liver collagen and serum prolidase were reached in the third week while the lowest levels of IGF-1 were found at the end of the third week.


CONCLUSIONS
Serum prolidase and IGF-1 either independently or in combination correlate with liver collagen content in hepatic fibrosis.
Tarçin
Orhan
O
Division of Gastroenterology, Gümüşsuyu Military Hospital, Istanbul, Turkey. ortarcin@hotmail.com
Gedik
Nursal
N
Karakoyun
Berna
B
Tahan
Veysel
V
Sood
Gagan
G
Celikel
Ciğdem
C
Tözün
Nurdan
N
D000818:Animals; D001652:Bile Ducts; D015415:Biomarkers / Q000097:blood; D004150:Dipeptidases / Q000097:blood*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D008026:Ligation; D008103:Liver Cirrhosis / Q000097:blood*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012044:Regression Analysis; D018709:Statistics, Nonparametric
D015415:Biomarkers; D007334:Insulin-Like Growth Factor I; D004150:Dipeptidases; C022449:proline dipeptidase