title: Identification of a neuron-specific human gene, KIAA1110, that is a guanine nucleotide exchange factor for ARF1.
abstract: To identify neuron-specific genes, we performed gene expression profiling, cDNA microarray and in silico ESTs (expressed sequence tags) analyses. We identified a human neuron-specific gene, KIAA1110 (homologue of rat synArfGEF (Po)), that is a member of the guanine nucleotide exchange factor (GEF) for the ADP-ribosylation factor (ARF). RT-PCR analysis showed that the KIAA1110 gene was expressed specifically in the brain among adult human tissues, whereas no apparent expression was observed in immature neural tissues/cells, such as fetal brain, glioma tissues/cells, and neural stem/precursor cells (NSPCs). The KIAA1110 protein was shown to be expressed in mature neurons but not in undifferentiated NSPCs. Immunohistochemical analysis also showed that KIAA1110 was expressed in neurons of the human adult cerebral cortex. Furthermore, the pull-down assay revealed that KIAA1110 has a GEF activity toward ARF1 that regulates transport along the secretion pathway. These results suggest that KIAA1110 is expressed specifically in mature neurons and may play an important role in the secretion pathway as a GEF for ARF1.
authors.affiliation: Neuroimmunology Research Group, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
authors.forename: Yujiro
authors.initials: Y
authors.lastname: Hattori
authors.forename: Shigeki
authors.initials: S
authors.lastname: Ohta
authors.forename: Kenji
authors.initials: K
authors.lastname: Hamada
authors.forename: Hisafumi
authors.initials: H
authors.lastname: Yamada-Okabe
authors.forename: Yonehiro
authors.initials: Y
authors.lastname: Kanemura
authors.forename: Yumi
authors.initials: Y
authors.lastname: Matsuzaki
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawakami
authors.forename: Masahiro
authors.initials: M
authors.lastname: Toda
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism*; D020662:Guanine Nucleotide Exchange Factors / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D009474:Neurons / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D014018:Tissue Distribution
chemical_list: D020662:Guanine Nucleotide Exchange Factors; C525068:IQSEC3 protein, human; D020823:ADP-Ribosylation Factor 1