title: Smart Immobilization of oligopeptides through electrochemical deposition onto surface.
abstract: A novel method for electrochemical molecular immobilization has been developed. Molecular immobilization on an electroconductive material surface is achieved by genetic and chemical introduction of a tag. The immobilization reaction is based on the remarkable phenomenon of neutral metal complex formation on a redox interface. For electrochemical immobilization, a metal coordinative peptide (EC tag) is introduced to the target molecule and is coordinated with a divalent metal ion. In the electrochemical immobilization process, the coordinated metal in the oligopeptide is reduced to the zero-valent metal state and is deposited on the electroconductive substrate. In the present study, we exploit our previous findings to carry out electrochemical peptide immobilization. This immobilization process can be modulated by an applied potential. Although the immobilized peptide is tightly attached the substrate, it can be removed by oxidation of deposited metal though application of an oxidation potential. The method can be employed for the immobilization of various molecules, e.g. proteins, peptides, and nano-materials, on electroconductive solid surfaces. The unique advantages of the present molecular immobilization method are the ease of application and the novel molecular modulations that are achievable.
authors.affiliation: Department of Biological Functions and Engineering, Kyushu Institute of Technology, Kitakyushu Science and Research Park, Fukuoka 808-0196, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sakamoto
authors.forename: Shinya
authors.initials: S
authors.lastname: Ikeno
authors.forename: Tamaki
authors.initials: T
authors.lastname: Kato
authors.forename: Norikazu
authors.initials: N
authors.lastname: Nishino
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Haruyama
mesh_terms: D004563:Electrochemistry / Q000379:methods*; D009842:Oligopeptides / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D009842:Oligopeptides