title: [Epidemiological investigation of an outbreak of acute gastroenteritis caused by human calicivirus in Lulong County].
abstract: OBJECTIVE
  To survey an outbreak of acute gastroenteritis in Lulong County and analyze the cause of the disease.
  
  
  METHODS
  Epidemiological methods were applied to investigate an outbreak of acute gastroenteritis occurred in June 2000 in Lulong County. Stool specimens were collected from diarrhea patients and were tested for human calicivirus by ELISA and RT-PCR. The products of RT-PCR were cloned and sequenced, then phylogenetic analysis was carried out.
  
  
  RESULTS
  In total, 736 farmers were surveyed, among them 134 had acute gastroenteritis, the attack rate was 18.20%, and one elderly patient died. The age of patients was from 1 to 77 years and the incidence of the disease among young people was higher with a peak in June 25 through 30. Six stool specimens were tested for caliciviruses by ELISA and 3 were positives, one of them was confirmed by RT-PCR and belonged to norovirus genotype GI/2. No other pathogens were detected.
  
  
  CONCLUSIONS
  Human calicivirus was confirmed to be the cause of the outbreak of acute gastroenteritis.
authors.affiliation: Lulong Center of Disease Control and Prevention, Hebei Province, Lulong, China.
authors.forename: Jin-Yu
authors.initials: JY
authors.lastname: Tang
authors.forename: Hua-Ping
authors.initials: HP
authors.lastname: Xie
authors.forename: Hai-Kuan
authors.initials: HK
authors.lastname: Hu
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Duan
authors.forename: Zhao-Yin
authors.initials: ZY
authors.lastname: Fang
mesh_terms: D000208:Acute Disease; D002139:Caliciviridae / Q000235:genetics / Q000302:isolation & purification; D017250:Caliciviridae Infections / Q000453:epidemiology*; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012367:RNA, Viral