Liposome destabilization induced by synthetic lipopeptides corresponding to envelope and non-structural domains of GBV-C/HGV virus. Conformational requirements for leakage.
Liposomes have been used primarily as a model system for studying biological membranes. Numerous chemical, biochemical and biophysical methods have been used to elucidate the various aspects of the interaction between proteins or peptides and phospholipids. Having in mind the potential use of synthetic lipopeptides as antiviral therapies and aiming for a better understanding of the molecular interaction of the GBV-C/HGV with liposomes as model membranes, epitopes of GBV-C/HGV located at the E2 (99-118) and NS3(440-460) regions were selected. Peptides were modified at the N-terminus with acyl chains of different length (C(14) and C(16)) yielding the corresponding myristoil and palmytoil lipopeptides. The main aim of the present study was to get insight into the membrane-interacting properties of the above-described synthetic lipopeptides and to study their inhibition of the capacity of perturbing model membranes of fusion peptide of HIV-1 using fluorescence spectroscopy. In an attempt to establish a relationship between peptide membrane activity and structure, we use Circular Dichroism (CD) and Fourier-Transform Infrared Spectroscopy (FTIR).
Fernández-Vidal
Mónica
M
Department of Peptide and Protein Chemistry, IIQAB-CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain.
Rojo
Núria
N
Herrera
Elena
E
Gómara
María José
MJ
Haro
Isabel
I
D000818:Animals; D019380:Anti-HIV Agents / Q000737:chemistry* / Q000494:pharmacology; D002213:Capsid / Q000737:chemistry; D002942:Circular Dichroism; D029407:GB virus C / Q000737:chemistry*; D015700:HIV Envelope Protein gp41 / Q000037:antagonists & inhibitors* / Q000737:chemistry; D006461:Hemolysis; D008081:Liposomes / Q000737:chemistry; D010446:Peptide Fragments / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000494:pharmacology; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000494:pharmacology
D019380:Anti-HIV Agents; D015700:HIV Envelope Protein gp41; D008081:Liposomes; D010446:Peptide Fragments; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; C107619:glycoprotein E2, GB virus C; C514787:gp41 protein, Human immunodeficiency virus 1