Lateral segregation of anionic phospholipids in model membranes induced by cytochrome P450 2B1: bi-directional coupling between CYP2B1 and anionic phospholipid.
The lateral segregation of anionic phospholipids phosphatidic acid (PA), phosphatidylinositol (PI), and phosphatidylserine (PS) was detected after addition of cytochrome P450 2B1 (CYP2B1). The tendency of lipid clustering was highly dependent on the type of anionic phospholipids examined. PA was the most highly clustered while PI and PS clustered to a lesser degree. Moreover, liposomes containing anionic phospholipids form anionic phospholipid-rich microdomains in the presence of CYP2B1. Anionic phospholipids (mostly notably PA) also increased the ability of CYP2B1 to bind to lipid monolayers. In addition to the ability of CYP2B1 to modulate the physical properties of the membrane, the membrane itself can have reciprocal effects on the activity and conformation of CYP2B1. The catalytic activity of CYP2B1 increased as a function of anionic phospholipid concentration and in the presence of 10 mol% PA, the activity increased by 85%. These results suggest a bi-directional coupling between the CYP2B1 and anionic phospholipids.
Kim
Keon-Hee
KH
School of Biological Sciences and Technology and Hormone Research Center, Chonnam National University, Gwangju 500-757, Republic of Korea.
Kim
Dong-Hyun
DH
Jang
Hyun-Hee
HH
Kim
Mihee
M
Kim
Dae-Hwan
DH
Kim
Joon-Sik
JS
Kim
Jeong-Il
JI
Chae
Ho Zoon
HZ
Ahn
Taeho
T
Yun
Chul-Ho
CH
D000838:Anions; D001665:Binding Sites; D019362:Cytochrome P-450 CYP2B1 / Q000737:chemistry* / Q000648:ultrastructure; D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D008565:Membrane Proteins / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D011485:Protein Binding
D000838:Anions; D008051:Lipid Bilayers; D008565:Membrane Proteins; D010743:Phospholipids; D019362:Cytochrome P-450 CYP2B1