title: Optimal design and validation of antiviral siRNA for targeting HIV-1.
abstract: We propose rational designing of antiviral short-interfering RNA (siRNA) targeting highly divergent HIV-1. In this study, conserved regions within HIV-1 genomes were identified through an exhaustive computational analysis, and the functionality of siRNAs targeting the highest possible conserved regions was validated. We present several promising antiviral siRNA candidates that effectively inhibited multiple subtypes of HIV-1 by targeting the best conserved regions in pandemic HIV-1 group M strains.
authors.affiliation: Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. y-naito@RNAi.jp
authors.forename: Yuki
authors.initials: Y
authors.lastname: Naito
authors.forename: Kyoko
authors.initials: K
authors.lastname: Nohtomi
authors.forename: Toshinari
authors.initials: T
authors.lastname: Onogi
authors.forename: Rie
authors.initials: R
authors.lastname: Uenishi
authors.forename: Kumiko
authors.initials: K
authors.lastname: Ui-Tei
authors.forename: Kaoru
authors.initials: K
authors.lastname: Saigo
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Takebe
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence; D030541:Databases, Genetic; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering; D012367:RNA, Viral