title: Age-related decrease in axonal transport measured by MR imaging in vivo.
abstract: Axonal transport is a crucial process for neuronal homeostasis and cell functions. In vitro studies have indicated transport rates decrease with age. Disruption of axonal transport has been implicated in age-associated neurodegenerative disorders. We hypothesized that aged rats would show decreased transport in the brain, which could be measured using in vivo manganese-enhanced MR imaging (Mn-MRI) and parametric estimation. Serial T1-weighted images were obtained at pre- and post-administration of MnCl(2) in rats scanned longitudinally (n=4) and in a separate aged group (n=3). Subtraction analysis was performed for group-wise statistical comparison on a pixel-by-pixel basis. Change in intensity over time was plotted for the olfactory bulb and anterior and posterior olfactory tract. Bulk transport of material was estimated over an initial 72 h. Tracer kinetic estimation of time-intensity data, based on a mass transport model, used intensity change in the bulb as input function for subsequent changes in the tract. Time to the peak of Mn(2+) flow was estimated for both anterior and posterior tracts. Results indicated age-related decreases in axonal transport rate and bulk transport of material in the olfactory tract of living rat brains. Longitudinally scanned, mid-age group was decreased by 58% and the aged group by 71% of young rate (neuronal transport=4.07+/-1.24 mm/h, 1.72+/-0.89 mm/h, and 1.16+/-0.18 mm/h for young, mid-age, and aged, respectively). Neuronal transport rate decreases correlated with increased age. The use of kinetic analysis combined with dynamic manganese enhanced MR imaging provides a unique opportunity to study this important neuronal process.
authors.affiliation: Washington National Regional Primate Center, Washington, USA.
authors.forename: Donna J
authors.initials: DJ
authors.lastname: Cross
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Flexman
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Anzai
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Maravilla
authors.forename: Satoshi
authors.initials: S
authors.lastname: Minoshima
mesh_terms: D000375:Aging / Q000502:physiology*; D000465:Algorithms; D000818:Animals; D001370:Axonal Transport / Q000502:physiology*; D001921:Brain / Q000254:growth & development / Q000502:physiology; D001923:Brain Chemistry; D007091:Image Processing, Computer-Assisted; D007700:Kinetics; D008279:Magnetic Resonance Imaging; D008297:Male; D008345:Manganese / Q000378:metabolism; D009833:Olfactory Pathways / Q000378:metabolism / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008345:Manganese