The histone demethylase, Jmjd1a, interacts with the myocardin factors to regulate SMC differentiation marker gene expression.
We and others have previously shown that the myocardin transcription factors play critical roles in the regulation of smooth muscle cell (SMC) differentiation marker gene expression. In a yeast 2-hybrid screen for proteins that interact with myocardin-related transcription factor-A (MRTF-A), we identified the histone 3 lysine 9 (H3K9)-specific demethylase, Jmjd1a. GST pull-down assays demonstrated that Jmjd1a bound all 3 myocardin family members, and further mapping studies showed that the jumonjiC domain of Jmjd1a was sufficient to mediate this interaction. Overexpression of Jmjd1a in multipotential 10T1/2 cells decreased global levels of di-methyl H3K9, stimulated the SM alpha-actin and SM22 promoters, and synergistically enhanced MRTF-A- and myocardin-dependent transactivation. Using chromatin immunoprecipitation assays, we also demonstrated that TGF-beta-mediated upregulation of SMC differentiation marker gene expression in 10T1/2 cells was associated with decreased H3K9 dimethylation at the CArG-containing regions of the SMC differentiation marker gene promoters. Importantly, knockdown of Jmjd1a in 10T1/2 cells and primary rat aortic SMCs by retroviral delivery of siRNA attenuated TGF-beta-induced upregulation of endogenous SM myosin heavy chain expression. These effects were concomitant with increased H3K9 dimethylation at the SMC differentiation marker gene promoters and with inhibition of MRTF-A-dependent transactivation of the SMC-specific transcription. These results suggest, for the first time, that SMC differentiation marker gene expression is regulated by H3K9 methylation and that the effects of the myocardin factors on SMC-specific transcription may involve the recruitment of Jmjd1a to the SMC-specific promoters.
Lockman
Kashelle
K
Department of Pathology, University of North Carolina, Chapel Hill, NC 27599-7525, USA.
Taylor
Joan M
JM
Mack
Christopher P
CP
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000502:physiology*; D006657:Histones / Q000378:metabolism; D056484:Jumonji Domain-Containing Histone Demethylases; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000201:enzymology; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000201:enzymology*; D009687:Nuclear Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism*; D051381:Rats; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D000099251:Myocardin
D015415:Biomarkers; D006657:Histones; D056484:Jumonji Domain-Containing Histone Demethylases; D009687:Nuclear Proteins; D010089:Oxidoreductases, N-Demethylating; D015534:Trans-Activators; D000099251:Myocardin; C511173:Mrtfa protein, mouse; C511045:Kdm3a protein, mouse