Manganese-enhanced MRI detected the gray matter lesions in the late phase of mild hypoxic-ischemic injury in neonatal rat.
This study aims to use manganese-enhanced MRI (MEMRI) to investigate the progression and permanence of the gray matter injuries in a neonatal rat model by mild hypoxic-ischemia (H-I) insult. Histological analyses were performed using staining for Mn superoxide dismutase (Mn-SOD) and glutamine synthetase (GS), which are Mn-binding enzymes against oxidative stress and glutamate excitotoxicity in neurodegeneration, and the standard hematoxylin and eosin (H&E). The transient changes associated with gray matter injuries in T(2)-weighted image (T2WI) and diffusion weighted image (DWI) in acute phase were shown to be detectable using MEMRI in late phase by systemic Mn(2+) administration, correlating with the local cell death, GS and Mn-SOD increase. Therefore, MEMRI may be a potentially useful diagnostic paradigm for detecting the gray matter injuries that are otherwise undetectable using the current MRI techniques in late phase of mild H-I injury.
Yang
Jian
J
Laboratory of Biomedical Imaging and Signal Processing, The University of Hong Kong, Pokfulam, Hong Kong.
Wu
Ed X
EX
D000818:Animals; D000831:Animals, Newborn; D002712:Chlorides / Q000008:administration & dosage*; D005974:Glutamate-Ammonia Ligase / Q000032:analysis; D020925:Hypoxia-Ischemia, Brain / Q000175:diagnosis* / Q000201:enzymology / Q000473:pathology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D017895:Manganese Compounds / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000032:analysis
D002712:Chlorides; D017895:Manganese Compounds; D013482:Superoxide Dismutase; D005974:Glutamate-Ammonia Ligase; C025340:manganese chloride