title: [Identification and clinical evaluation of lung cancer serum biomarker L-lactate dehydrogenase B].
abstract: OBJECTIVE
  To identify potential biomarkers related with lung cancer metastasis.
  
  
  METHODS
  Conditional media proteins collected from a primary non-small cell lung cancer cell line (NSCLC) NCI-H226 and its brain metastatic subline H226Br were analyzed by SDS-PAGE and MALDI-TOF-MS. Twelve biomarkers were identified, of which LDHB was significantly up-regulated in H226Br cell and was further validated using ELISA in sera including 105 lung cancer samples, 41 pneumonia and pulmonary tuberculosis samples and 65 healthy samples.
  
  
  RESULTS
  The levels of LDHB were specifically elevated in NSCLC sera [A value 0.485 (0 - 1.415)] compared with pneumonia and pulmonary tuberculosis [A value 0.187 (0 - 0.609), P < 0.01] and healthy group [A value, 0.159 (0 - 0.524), P < 0.01] and were progressively increased with the clinical stage. At the cutoff point 0.260 (A value) on the ROC curve, the sensitivity, specificity and total accuracy of LDHB were 81%, 70% and 76% respectively.
  
  
  CONCLUSIONS
  These findings demonstrated that secretome could open up a possibility to identify novel biomarkers related with cancer occurrence and metastasis.
authors.affiliation: Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, Beijing Normal University, Beijing 100875, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: An-jian
authors.initials: AJ
authors.lastname: Xu
authors.forename: Ji-fu
authors.initials: JF
authors.lastname: Liu
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Wu
authors.forename: Ling-yun
authors.initials: LY
authors.lastname: Huang
authors.forename: Da-cheng
authors.initials: DC
authors.lastname: He
authors.forename: Xue-yuan
authors.initials: XY
authors.lastname: Xiao
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood* / Q000378:metabolism; D008175:Lung Neoplasms / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D040901:Proteomics / Q000379:methods; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000378:metabolism / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D017077:Culture Media, Conditioned; D007770:L-Lactate Dehydrogenase