Characteristics of non-typhoidal Salmonella isolates from human and broiler-chickens in southwestern Seoul, Korea.
Non-typhoidal Salmonella (NTS) is an important commensal microorganism. The purpose of this study was to determine the epidemiological relation between NTS isolates from livestock and NTS isolates from human by analyzing antimicrobial susceptibilities and performing molecular typing. We determined the serotypes of 36 human clinical isolates and 64 livestock isolates, performed antimicrobial susceptibility testing against 8 antibiotics, and determined the molecular types of isolated NTS spp. by pulsed field gel electrophoresis (PFGE). In human isolates, S. enteritidis was the most common serotype (17 isolates; 47.2%) and S. typhimurium the second most (8 isolates; 22.2%). In livestock isolates, S. typhimurium was the most common serotype (15 isolates; 23.44%), and S. enteritidis was the second most (14 isolates; 21.88%). Ampicillin and tetracycline resistance were 50% (32/64 isolates) each among broiler-chicken NTS isolates. No human or livestock NTS isolates showed resistance to ciprofloxacin, TMP-SMX, or ceftriaxone. However, 19.4% (7/36) and 46.8% (30/64) of the human and livestock NTS isolates were resistant to nalidixic acid (MIC > or = 16 mg/mL), respectively. The presence of the three identical PFGE molecular types from human and broiler-chicken NTS isolates suggests the possibility of transmission from livestock to humans.
Cheong
Hee Jin
HJ
Department of Internal Medicine, Korea University Medical College, Seoul, Korea.
Lee
Yeon Joo
YJ
Hwang
In Sook
IS
Kee
Sae Yoon
SY
Cheong
Hye Won
HW
Song
Joon Young
JY
Kim
Jun Man
JM
Park
Yong Ho
YH
Jung
Ji-Hun
JH
Kim
Woo Joo
WJ
D000328:Adult; D000818:Animals; D002645:Chickens; D016000:Cluster Analysis; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D007723:Korea; D008297:Male; D009268:Nalidixic Acid / Q000494:pharmacology; D012480:Salmonella Infections / Q000453:epidemiology / Q000378:metabolism / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000453:epidemiology / Q000378:metabolism / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000378:metabolism; D012486:Salmonella typhimurium / Q000378:metabolism*; D012703:Serotyping
D009268:Nalidixic Acid