title: [Epidemiological and virological aspects Rotavirus diarrhoea in Abidjan, Côte d'Ivoire (1997-2000)].
abstract: Viruses, mainly rotaviruses are aetiological agents in more than 80% of the cases of acute diarrhoea in children. In order to determine the epidemiological characteristics and genotypes of human rotaviruses involved in gastroenteritis in diarrheic children aged from 0 to 5 years old in Abidjan, 642 specimens of stools were collected between 1997 and 2000 in the urban health centres and University Teaching Hospitals in Abidjan. The antigenic detection of rotaviruses carried out by ELISA test was followed by the antigenic (VP6 sub-groups) and molecular characterization: polyacrylamide gel electrophoresis and genetic typing. The general prevalence of Rotavirus diarrhoea was 27.9%. Among the children who were found positive, those whose age ranged from 0 to 11 months old accounted for 45.8% against 41.3% and 12.9% for those whose age ranged from 1 to 2 and 3 to 5 years old respectively proving thus the precocity of rotavirus infection. From an electrophoretypical and antigenic point of view 74.5% of 141 extracts of RNA had a "long" profile and belonged to the VP6 II sub-group against 24.8% of "short" profile belonging to sub-group I. The electrophoretypes with short profile were identified in majority in infants whose age ranged from 0 to 2 years old. Out of the P genotypes identified, the P [8] genotype (59.6%) was predominant followed by the P [6] genotype (26.2%), P [4] (2.8%) and one mosaic genotype P[6,8] which represented 11.4%. These results will need to be completed by the determination of VP7 genotypes in order to provide interesting information on rotaviruses before the introduction of anti-Rotavirus vaccines in the country.
authors.affiliation: Département des virus ẃpidémiques, Institut Pasteur de Côte d'Ivoire, BP 490 Abidjan 01, Côte d'Ivoire. akouamc@yahoo.fr
authors.forename: C
authors.initials: C
authors.lastname: Akoua-Koffi
authors.forename: V
authors.initials: V
authors.lastname: Akran
authors.forename: I
authors.initials: I
authors.lastname: Peenze
authors.forename: V
authors.initials: V
authors.lastname: Adjogoua
authors.forename: M C
authors.initials: MC
authors.lastname: de Beer
authors.forename: A D
authors.initials: AD
authors.lastname: Steele
authors.forename: M
authors.initials: M
authors.lastname: Dosso
authors.forename: A
authors.initials: A
authors.lastname: Ehouman
mesh_terms: D000367:Age Factors; D000956:Antigens, Viral / Q000032:analysis; D036022:Capsid Proteins / Q000032:analysis; D002675:Child, Preschool; D007560:Cote d'Ivoire / Q000453:epidemiology; D003968:Diarrhea, Infantile / Q000453:epidemiology / Q000821:virology*; D004591:Electrophoresis, Polyacrylamide Gel; D016021:Epidemiologic Studies; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology; D005838:Genotype; D006801:Humans; D007223:Infant; D015995:Prevalence; D016601:RNA-Binding Proteins / Q000032:analysis; D012401:Rotavirus / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology*; D017361:Viral Nonstructural Proteins / Q000032:analysis
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D016601:RNA-Binding Proteins; C060339:VP4 protein, Rotavirus; C061332:VP6 protein, Rotavirus; D017361:Viral Nonstructural Proteins; C079234:NS35 protein, rotavirus