Expression analysis of Mx protein and evaluation of its antiviral activity against sole aquabirnavirus in SAF-1 and TV-1 cell lines.
The transcription of Mx mRNA after poly I:C induction and sole aquabirnavirus infection has been analysed in SAF-1 and TV-1 cells (derived from gilt-head seabream and turbot, respectively). Both cell lines were stimulated with 10 microg ml(-1) poly I:C and Mx mRNA was analysed by a specific RT-PCR at several times post-induction. The results showed a high level of Mx expression from 12 to 120 h after induction in SAF-1 cells, whereas in TV-1 cells Mx mRNA was only detected at 12 and 24h. The treatment with different concentrations of poly I:C showed that TV-1 cells are less sensitive to this inductor than the SAF-1 cell line. The antiviral activity derived from poly I:C induction has been clearly demonstrated against sole aquabirnavirus on both cell lines. The inoculation of sole aquabirnavirus resulted in the Mx mRNA transcription at 48, 72, and 96 h post-infection (p.i.) in SAF-1 cells. On the contrary, inoculated TV-1 cells only showed a faint Mx mRNA band at 24 and 48 h p.i. This study has established different patterns of Mx expression in both cells under study as a consequence of the poly I:C induction and sole aquabirnavirus infection, and it shows that gilt-head seabream and turbot Mx inhibit sole aquabirnavirus replication.
García-Rosado
Esther
E
Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Campus Teatinos, 29071 Málaga, Spain.
Alonso
M Carmen
MC
Béjar
Julia
J
Manchado
Manuel
M
Cano
Irene
I
Borrego
Juan J
JJ
D000818:Animals; D019201:Aquabirnavirus / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D017756:Aquaculture; D018175:Birnaviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D002460:Cell Line; D005393:Fish Diseases / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D029941:Fish Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D005412:Flatfishes; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D064547:Myxovirus Resistance Proteins; D011070:Poly I-C / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D021541:Sea Bream; D014158:Transcription, Genetic; D014779:Virus Replication
D029941:Fish Proteins; D064547:Myxovirus Resistance Proteins; D012333:RNA, Messenger; D019204:GTP-Binding Proteins; D011070:Poly I-C