title: Birth of a healthy boy after fertilization of cryopreserved oocytes with cryopreserved testicular spermatozoa from a man with nonmosaic Klinefelter syndrome.
abstract: OBJECTIVE
  To report the first birth with frozen spermatozoa and frozen oocytes in a case of nonmosaic Klinefelter syndrome.
  
  
  METHODS
  Case report.
  
  
  METHODS
  Private IVF clinics and a university hospital.
  
  
  METHODS
  A 32-year-old woman and a 33-year-old man.
  
  
  METHODS
  Testicular sperm extraction and cryopreservation, oocyte recovery and cryopreservation, intracytoplasmic sperm injection.
  
  
  METHODS
  Assessment of fertilization and chromosomal analysis of newborn child.
  
  
  RESULTS
  Three oocytes were injected and one embryo was obtained, resulting in the birth of a normal (46,XY) boy.
  
  
  CONCLUSIONS
  Cryopreserved spermatozoa from a patient with nonmosaic Klinefelter syndrome can form normal embryos even with cryopreserved oocytes.
authors.affiliation: Center for Reproductive Medicine, European Hospital, Rome, Italy.
authors.forename: Ermanno
authors.initials: E
authors.lastname: Greco
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacobelli
authors.forename: Laura
authors.initials: L
authors.lastname: Rienzi
authors.forename: Giuseppe F Menchini
authors.initials: GF
authors.lastname: Fabris
authors.forename: Nicola
authors.initials: N
authors.lastname: Tesorio
authors.forename: Jan
authors.initials: J
authors.lastname: Tesarik
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007713:Klinefelter Syndrome / Q000150:complications* / Q000473:pathology; D050498:Live Birth; D008297:Male; D054315:Oocyte Retrieval; D009865:Oocytes; D010062:Ovulation Induction; D011247:Pregnancy; D012662:Semen Preservation; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa; D013737:Testis / Q000473:pathology*; D016896:Treatment Outcome