Assessment of protein-incorporated arginine methylation in biological specimens by CZE UV-detection.
Protein arginine methyltransferases methylate post-translationally arginine residues in proteins to synthesize monomethylarginine (MMA), asymmetric dimethylarginine (ADMA), or symmetric dimethylarginine. Protein arginine methylation is involved in the regulation of signal transduction, RNA export, and cell proliferation. Moreover, upon proteolysis, arginines are released into the cytosol in which they exert important biological effects. Both MMA and ADMA are inhibitors of nitric oxide synthase and especially elevated levels of ADMA are associated with endothelial dysfunction and cardiovascular disease. Quantification of these analytes is commonly performed by HPLC after sample cleanup and derivatization. We propose a CE method in which these steps have been avoided and the procedure for sample preparation has been simplified. After acidic hydrolysis of proteins, samples were dried, resuspended in water, and directly injected in CE. A baseline separation of analytes was reached in a 60 cm x 75 microm id uncoated silica capillary, by using a Tris-phosphate run buffer at pH 2.15. This method allows an accurate assessment of protein arginine methylation degree in different biological samples such as whole blood, plasma, red blood cells, cultured cells, and tissue. Moreover, its good sensitivity permits to evaluate the methylation of a single protein type after the opportune purification steps. A method applicability concerns both clinical laboratories, where the evaluation of blood protein from numerous samples could be rapidly performed, and research laboratories where the factors affecting the arginine protein methylation degree could be easily studied.
Zinellu
Angelo
A
Department of Biomedical Sciences, Chair of Clinical Biochemistry, University of Sassari, Sassari, Italy. angelozinellu@libero.it
Sotgia
Salvatore
S
Scanu
Bastianina
B
Formato
Marilena
M
Deiana
Luca
L
Carru
Ciriaco
C
D001120:Arginine / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry*; D001798:Blood Proteins / Q000032:analysis / Q000737:chemistry; D002478:Cells, Cultured / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D004730:Endothelium, Vascular / Q000737:chemistry; D005240:Feasibility Studies; D006801:Humans; D008745:Methylation; D011499:Protein Processing, Post-Translational; D011484:Protein-Arginine N-Methyltransferases / Q000737:chemistry*; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D013056:Spectrophotometry, Ultraviolet; D014471:Umbilical Veins / Q000737:chemistry
D001798:Blood Proteins; D011506:Proteins; C024917:symmetric dimethylarginine; C018524:N,N-dimethylarginine; D001120:Arginine; D011484:Protein-Arginine N-Methyltransferases