[Experimental study of immunostimulatory DNA sequence in conjunction with an allergen increasing allergic rhinitis in rats].
OBJECTIVE
To observe the suppressive effects of immunostimulatory DNA sequences (ISS) in conjunction with dermato phalloides farinae allergen (Df) on nasal cavity inflammation in rats of experimental allergic rhinitis (AR).


METHODS
Thirty-two rats were divided into 4 groups: group ISS(A), group ISS+ Df (B), group Df (C) and group normal saline (D). Rats in groups A,B and C were sensitized and challenged with Df. Blood samples were obtained every week for six weeks, Df specific IgE was measured by ELISA. The nasal mucosa were studied pathologically. The levels of serum interferon gamma (IFN-gamma), IL-12, IL-4 and IL-5 were determined by ELISA.


RESULTS
The serum level of sIgE in group B was significantly lower than that in group C in six weeks, but that in group A was not significantly different to that in group C. The mean levels of serum IFN-gamma and IL-12 in A, B group were significantly higher than in C group (P < 0.01). But IL-4 and IL-5 level was much lower (P < 0.01).


CONCLUSIONS
ISS+ Df have inhibited effectiveness on allergic nasal cavity inflammation in rats and its action time is 6 weeks.
Zhang
Shifu
S
Department of Otorhinolaryngology, the Affiliated Hospital of Guangdong Medical College, Zhanjiang, 524001, China. zhshifu@gdmc.edu.cn
Cheng
Lilan
L
Hou
Gan
G
Tan
Jiquan
J
D000276:Adjuvants, Immunologic; D000485:Allergens; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000276:immunology; D007114:Immunization; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D015848:Interleukin-5 / Q000097:blood; D008297:Male; D039981:Pyroglyphidae / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology / Q000628:therapy*
D000276:Adjuvants, Immunologic; D000485:Allergens; D015848:Interleukin-5; D015847:Interleukin-4; D007371:Interferon-gamma; D004247:DNA