Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA-microgel bioassays.
We demonstrate that DNA oligonucleotides covalently coupled to colloidal microgel can be manipulated by T4 DNA ligase for DNA ligation and by Phi29 DNA polymerase for rolling circle amplification (RCA). We also show that the long single-stranded RCA product can generate intensive fluorescence upon hybridization with complementary fluorescent DNA probe. We believe DNA-microgel conjugates can be explored for the development of DNA based bioassays and biosensors.
Ali
Md Monsur
MM
Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main St. W., Hamilton, ON, L8N 3Z5, Canada.
Su
Shunxing
S
Filipe
Carlos D M
CD
Pelton
Robert
R
Li
Yingfu
Y
D001483:Base Sequence; D001681:Biological Assay / Q000379:methods; D015374:Biosensing Techniques / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D011088:DNA Ligases / Q000737:chemistry* / Q000378:metabolism; D015342:DNA Probes / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism; D005782:Gels / Q000737:chemistry*; D008969:Molecular Sequence Data
D015342:DNA Probes; D005782:Gels; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D011088:DNA Ligases