Population structures of two genotypes of Vibrio vulnificus in oysters (Crassostrea virginica) and seawater.
Vibrio vulnificus biotype 1 strains can be classified into two genotypes based on the PCR analysis of variations in the virulence-correlated gene (vcg). Genotype has been correlated with human infection for 90% of isolates from human cases having the vcgC sequence type and 87% of environmental strains having the vcgE variant. In this study we examined the dynamics of V. vulnificus populations and the distribution of the two genotypes recovered from oysters and surrounding estuarine wasters. Analysis of 880 isolates recovered from oysters showed a disparity in the ratio of the two genotypes, with those of the vcgE (E) genotype accounting for 84.4% of the population. In contrast, 292 isolates recovered from the waters surrounding the oyster sites revealed an almost equal distribution of the two genotypes. The levels of vcgC (C genotype) strains from both sources increased as a percentage of the population as water temperatures increased, while no culturable V. vulnificus cells were recovered from December through February. Our results suggest that there is a selective advantage for strains of the E genotype within oysters while survival of the C genotype strains may be favored by increased water column temperatures. These data suggest that the low incidence of infections may be due to the comparatively rare consumption of an oyster that contains a greater number of V. vulnificus vcgC genotype strains than of vcgE genotype strains. Levels of the two genotypes as well as seasonal dynamics within both oyster tissue and the surrounding waters may aid in identifying risk factors associated with human infection.
Warner
Elizabeth
E
Department of Biology, University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
Oliver
James D
JD
D000818:Animals; D010125:Ostreidae / Q000382:microbiology*; D012621:Seasons; D012623:Seawater / Q000382:microbiology*; D014735:Vibrio Infections / Q000382:microbiology*; D041261:Vibrio vulnificus / Q000145:classification / Q000235:genetics* / Q000254:growth & development*; D037521:Virulence Factors / Q000235:genetics
D037521:Virulence Factors