A PCR-restriction fragment length polymorphism assay to genotype human metapneumovirus.
Human metapneumovirus (hMPV) genotypes A and B show epidemiological and probably clinical differences. This report describes a fast and simple PCR-restriction fragment length polymorphism (PCR-RFLP) assay, involving digestion of the fusion protein gene with Tsp509I, that allows lineages A1, A2, B1 and B2 to be distinguished. The assay should help in elucidating the epidemiology of hMPV, and possibly in predicting the severity of clinical infection.
Montes
M
M
Servicio de Microbiología, Hospital Donostia, Basque Country University, San Sebastián, Spain.
Vicente
D
D
Esnal
O
O
Cilla
G
G
Pérez-Trallero
E
E
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D002648:Child; D005838:Genotype; D006801:Humans; D029121:Metapneumovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017720:Molecular Epidemiology / Q000379:methods; D014760:Viral Fusion Proteins / Q000235:genetics
D014760:Viral Fusion Proteins