title: Structural properties of the human acidic ribosomal P proteins forming the P1-P2 heterocomplex.
abstract: The ribosome has a morphologically distinct structural feature called the stalk, recognized as a vital element for its function. The ribosomal P proteins constitute the main part of the eukaryotic ribosomal stalk, forming a pentameric structure P0-(P1-P2)(2). The group of P1/P2 proteins in eukaryotes is very diverse, and in spite of functional and structural similarities they do not fully complement one another, probably constituting an adaptive feature of the ribosome from a particular species to diverse environmental conditions. The functional differences among the P1/P2 proteins were analysed in vivo several times; however, a thorough molecular characterization was only done for the yeast P1/P2 proteins. Here, we report a biophysical analysis of the human P1 and P2 proteins, applying mass spectrometry, CD and fluorescence spectroscopy, cross-linking and size exclusion chromatography. The human P1/P2 proteins form stable heterodimer, as it is the case for P1/P2 from yeast. However, unlike the yeast complex P1A-P2B, the human P1-P2 dimer showed a three-state transition mechanism, suggesting that an intermediate species may exist in solution.
authors.affiliation: Department of Molecular Biology, Maria Curie-Skłodowska University, Akademicka 19, Lublin, Poland.
authors.forename: Przemysław
authors.initials: P
authors.lastname: Grela
authors.forename: Justyna
authors.initials: J
authors.lastname: Sawa-Makarska
authors.forename: Yuliya
authors.initials: Y
authors.lastname: Gordiyenko
authors.forename: Carol V
authors.initials: CV
authors.lastname: Robinson
authors.forename: Nikodem
authors.initials: N
authors.lastname: Grankowski
authors.forename: Marek
authors.initials: M
authors.lastname: Tchórzewski
mesh_terms: D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D013058:Mass Spectrometry; D011487:Protein Conformation; D012269:Ribosomal Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D012269:Ribosomal Proteins