Aberrant expression of histo-blood group A type 3 antigens in vascular endothelial cells in inflammatory sites.
Histo-blood group ABH antigens are widely distributed in human tissues. The epitopes of ABH antigens are carried by at least four different peripheral core isotypes of internal carbohydrate backbones (type 1-4). Each type of ABH antigen is expressed tissue specifically, and aberrant expression of ABH antigens is often observed during oncogenesis. We immunohistochemically examined the expression of A type 3 antigens in wounded and diseased skin tissues (A and AB blood groups). In uninjured skin, the expression of A type 3 antigens was restricted to the eccrine sweat gland. In addition to the sweat glands, A type 3 antigens were found in vascular endothelial cells of the wound sites. The extent of A type 3 antigens expression related to postinfliction intervals. A significantly higher expression rate of A type 3 antigens in endothelial cells was also observed in diseased skin, suggesting that inflammation might induce A type 3 antigen expression in endothelial cells. Double-color immunofluorescence staining of the specimens showed that von Willebrand factor (vWF) was a core-protein of A type 3 determinants aberrantly expressed in endothelial cells in inflamed tissues, suggesting that aberrant expression of A type 3 antigens is involved in stabilization of vWF in inflammation.
Nosaka
Mizuho
M
Department of Forensic Medicine, Wakayama Medical University, Wakayama, Japan.
Ishida
Yuko
Y
Tanaka
Aki
A
Hayashi
Takahito
T
Miyashita
Tomoko
T
Kaminaka
Chikako
C
Eisenmenger
Wolfgang
W
Furukawa
Fukumi
F
Kimura
Akihiko
A
D000017:ABO Blood-Group System / Q000096:biosynthesis*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D042783:Endothelial Cells / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000378:metabolism; D008875:Middle Aged; D012867:Skin / Q000098:blood supply* / Q000293:injuries; D012871:Skin Diseases / Q000378:metabolism*; D014841:von Willebrand Factor / Q000096:biosynthesis
D000017:ABO Blood-Group System; D014841:von Willebrand Factor