title: pH-dependent fluorescence of uncharged benzothiazole-based dyes binding to DNA.
abstract: Uncharged benzothiazole-based dyes were synthesized, and their fluorescence properties were examined. These fluorescent dyes showed an intense fluorescence in aqueous solution in the presence of DNA. In addition, the fluorescence intensities were pH dependent, while those of the positively charged dyes were nearly independent of pH. The pH-dependent photophysical behavior suggests that interaction of protonated dyes with DNA results in high intensities of fluorescent emission.
authors.affiliation: The Frontier Research System, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama, 351-0198, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Ikeda
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
mesh_terms: D000818:Animals; D052160:Benzothiazoles / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D001665:Binding Sites; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D004563:Electrochemistry; D005399:Fishes; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D015394:Molecular Structure; D018462:Photobiology; D013050:Spectrometry, Fluorescence
chemical_list: D052160:Benzothiazoles; D005456:Fluorescent Dyes; D004247:DNA