Expression of matrix metalloproteinases-2 and -9 and RECK during alveolar bone regeneration in rat.
MMPs are endopeptidases that play a pivotal role in ECM turnover. RECK is a single membrane-anchored MMP-regulator. Here, we evaluated the temporal and spatial expression of MMP-2, MMP-9, and RECK during alveolar bone regeneration. The maxillary central incisor of Wistar rats was extracted and the animals were killed at 1, 3, 7, 10, 14, 21, 28, and 42 days post-operatively (n = 3/period). The hemimaxillae were collected, demineralized and embedded in paraffin. Immunohistochemical analysis was performed by the immunoperoxidase technique with polyclonal antibodies. On day 1, polymorphonuclear cells in the blood clot presented mild immunolabeling for MMPs. During bone remodeling, osteoblasts facing new bone showed positive staining for gelatinases and RECK in all experimental periods. MMPs were also found in the connective tissue and endothelial cells. Our results show for the first time that inactive and/or active forms of MMP-2, MMP-9 and RECK are differentially expressed by osteogenic and connective cells during several events of alveolar bone regeneration. This may be important for the replacement of the blood clot by connective tissue, and in the formation, maturation and remodeling of new bone.
Accorsi-Mendonça
Thais
T
Department of Endodontics, Bauru Dental School, University of Sao Paulo, Bauru, SP, Brazil.
Paiva
Katiúcia Batista da Silva
KB
Zambuzzi
Willian Fernando
WF
Cestari
Tânia Mary
TM
Lara
Vanessa Soares
VS
Sogayar
Mari Cleide
MC
Taga
Rumio
R
Granjeiro
José Mauro
JM
D000818:Animals; D001861:Bone Regeneration; D058851:GPI-Linked Proteins; D007124:Immunoenzyme Techniques; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000378:metabolism*; D008437:Maxilla; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D025521:Tumor Suppressor Proteins
D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; C529735:Reck protein, rat; D025521:Tumor Suppressor Proteins; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; D020780:Matrix Metalloproteinase 9