Controllable self-assembly from fibrinogen-gold (fibrinogen-Au) and thrombin-silver (thrombin-Ag) nanoparticle interaction.
Fibrinogen conjugated gold nanoparticles (fibrinogen-Au) and thrombin conjugated silver nanoparticles (thrombin-Ag) were synthesized by heating (90 degrees C) the proteins (50 microg protein/ml) with 1mM AgNO(3) or AuCl(3). The resultant particles were harvested and examined by flow cytometry, scanning electron microscopy (SEM), transmission emission microscopy (TEM), optical microscopy and dynamic light scattering. SEM and TEM images revealed that the fibrinogen-Au and thrombin-Ag particles interacted. The emergent bio-nanoconjugate population could be controlled by addition of thrombin-Ag. The method may be exploited in parametrizing coagulation factors and other clinically important protein-protein interactions.
Roy
Shibsekhar
S
Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700 019, India.
Dasgupta
Anjan Kumar
AK
D004583:Electrons; D005337:Fibrin / Q000737:chemistry / Q000378:metabolism; D005340:Fibrinogen / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure*; D005434:Flow Cytometry; D006046:Gold / Q000737:chemistry*; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D011485:Protein Binding; D012834:Silver / Q000737:chemistry*; D013917:Thrombin / Q000737:chemistry / Q000648:ultrastructure*
D012834:Silver; D006046:Gold; D005337:Fibrin; D005340:Fibrinogen; D013917:Thrombin