title: Preparation of hydroxyapatite-nanocrystal-coated stainless steel, and its cell interaction.
abstract: Calcined nanocrystals of hydroxyapatite (HAp) having spherical or rod-shaped morphologies were coated through covalent linkage on a type 316L stainless steel substrate, which was chemically modified by the graft polymerization of gamma-methacryloxypropyl triethoxysilane (MPTS) at 70-110 degrees C. The grafting of poly(MPTS) on the substrate was confirmed by X-ray photoelectron spectroscopy (XPS) and attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR). In order to coat the substrate with the HAp crystals through covalent linkage, the reactionbetween the alkoxysilyl groupsin the poly (MPTS) grafted on the substrate and the OH groups on the HAp crystals was conducted at 80 degrees C. The poly(MPTS)-grafted substrate was strongly coated with the HAp nanocrystals, although the HAp crystals adsorbed physically on the original substrate without poly(MPTS) grafting were removed by ultrasonic treatment. Human umbilical vein endothelial cells (HUVEC) adhered in larger numbers on the HAp-coated stainless steel substrate as compared with the original substrate after 24 h of initial incubation. The number of HUVEC adhered on the rod-shaped HAp-coated substrate was not significantly different from that on the spherical HAp-coated substrate under the present conditions.
authors.affiliation: Department of Bioengineering, Advanced Medical Engineering Center, National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okada
authors.forename: Miwa
authors.initials: M
authors.lastname: Masuda
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Tanaka
authors.forename: Kunio
authors.initials: K
authors.lastname: Miyatake
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kuroda
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Furuzono
mesh_terms: D002448:Cell Adhesion; D002450:Cell Communication; D002478:Cells, Cultured; D017886:Durapatite / Q000378:metabolism*; D006801:Humans; D008422:Materials Testing; D053758:Nanoparticles / Q000648:ultrastructure; D012821:Silanes / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013057:Spectrum Analysis; D013193:Stainless Steel
chemical_list: D012821:Silanes; D013193:Stainless Steel; D017886:Durapatite