Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets.
The response of rainbow trout (Oncorhynchus mykiss, Walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (APR). Proteomic analysis by two-dimensional electrophoresis (2D) concurrently with mass spectrometry was used to detect APR related proteins in rainbow trout serum following feeding with probiotics Aeromonas sobria GC2 and Bacillus sp. JB-1. Three candidate proteins increased following use of GC2, and were putatively identified as NADH dehydrogenase, dystrophin and mKIAA0350. Conversely, one of the proteins, which were induced following use of JB-1 was identified as transferrin.
Brunt
Jason
J
School of Life Sciences, John Muir Building, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, Scotland, UK.
Hansen
Rasmus
R
Jamieson
Derek J
DJ
Austin
Brian
B
D000210:Acute-Phase Reaction / Q000097:blood* / Q000276:immunology; D000333:Aeromonas / Q000276:immunology; D000818:Animals; D017756:Aquaculture / Q000379:methods; D001407:Bacillus / Q000276:immunology; D016189:Dystrophin / Q000097:blood; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D005393:Fish Diseases / Q000097:blood* / Q000276:immunology / Q000517:prevention & control; D009245:NADH Dehydrogenase / Q000097:blood; D017686:Oncorhynchus mykiss / Q000097:blood* / Q000276:immunology / Q000378:metabolism; D019936:Probiotics / Q000494:pharmacology*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary; D014168:Transferrin / Q000378:metabolism
D016189:Dystrophin; D014168:Transferrin; D009245:NADH Dehydrogenase