Radioiodination of mouse anti-III beta-tubulin antibodies and their evaluation with respect to their use as diagnostic agents for peripheral neuropathies.
The monoclonal antibody TU-20 and its scFv fragment were radiolabeled with 125 I in order to develop new imaging agents against the specific neuronal marker III beta-tubulin. The reaction via chloramine-T using thiosulfate as a stopping reductant was determined as the most convenient way for radioiodination. The preserved immunological properties of radioiodinated species were estimated by ELISA, electrophoresis, and immunohistochemistry with autoradiography. Biodistribution studies revealed a different behavior of radioiodinated TU-20 and its scFv.
Svecová
Helena
H
Nuclear Physics Institute of the ASCR, v.v.i., CZ-250 68 Rez near Prague, Czech Republic. svecova@ujf.cas.cz
Kleinová
Veronika
V
Seifert
Daniel
D
Chaloupková
Hana
H
Bäurle
Jörg
J
Kranda
Karel
K
Král
Vlastimil
V
Fiser
Miroslav
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology / Q000493:pharmacokinetics; D001345:Autoradiography; D018796:Immunoconjugates / Q000737:chemistry / Q000276:immunology / Q000493:pharmacokinetics; D007128:Immunoglobulin Fragments / Q000737:chemistry / Q000276:immunology; D007150:Immunohistochemistry; D007457:Iodine Radioisotopes / Q000737:chemistry*; D007553:Isotope Labeling; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010523:Peripheral Nervous System Diseases / Q000000981:diagnostic imaging* / Q000378:metabolism; D011877:Radionuclide Imaging; D014018:Tissue Distribution; D014404:Tubulin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D018796:Immunoconjugates; D007128:Immunoglobulin Fragments; D007457:Iodine Radioisotopes; D014404:Tubulin; C462941:beta3 tubulin, mouse