[Anti-oxidative responses of Agrocybe aegerita mycelia to cadmium stress].
This paper studied the effects of different concentration Cd on the anti-oxidative enzyme activities and glutathione content in Agrocybe aegerita cultivated in liquid medium. The results indicated that at low concentrations of Cd, the test enzyme activities increased with increasing Cd concentration, being the maximum at 0.1 mmol x L(-1) for CAT, at 0.2 mmol x L(-1) for POD, GR and LOX, and at 0.4 mmol x L(-1) for SOD. At 1.6 mmol x L(-1) of Cd, the activities of POD, CAT and SOD were inhibited markedly. 0.4-1.6 mmol x L(-1) of Cd resulted in an increase of glutathione content, but glutathione disulfide content was less affected. The ascorbate acid content and APX activity were too low to be detectable. The PAGE analysis revealed that 0.1-0.8 mmol x L(-1) of Cd induced the additional isozyme bands of POD, EST and LOX, and increased the intensity of the constitutive isozymes of CAT and SOD. 1.6 mmol x L(-1) of Cd decreased the intensity of the isozymes of POD, CAT and SOD significantly.
Wang
Song-Hua
SH
College of Life Science, Anhui Sciences and Technology University, Bengbu 233100, Anhui, China. shwang70@yahoo.com.cn
Zhang
Hua
H
Fu
Man-Qin
MQ
Chen
Qing-Yu
QY
Zhou
Zheng-Yi
ZY
Lu
Xiao-Min
XM
D000363:Agaricales / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D025282:Mycelium / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D002104:Cadmium; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione