title: Rapid control of protein level in the apicomplexan Toxoplasma gondii.
abstract: Analysis of gene function in apicomplexan parasites is limited by the absence of reverse genetic tools that allow easy and rapid modulation of protein levels. The fusion of a ligand-controlled destabilization domain (ddFKBP) to a protein of interest enables rapid and reversible protein stabilization in T. gondii. This allows an efficient functional analysis of proteins that have a dual role during host cell invasion and/or intracellular growth of the parasite.
authors.affiliation: Hygieneinstitut, Department of Parasitology, University Hospital Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany.
authors.forename: Angelika
authors.initials: A
authors.lastname: Herm-Götz
authors.forename: Carolina
authors.initials: C
authors.lastname: Agop-Nersesian
authors.forename: Sylvia
authors.initials: S
authors.lastname: Münter
authors.forename: Joshua S
authors.initials: JS
authors.lastname: Grimley
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Wandless
authors.forename: Friedrich
authors.initials: F
authors.lastname: Frischknecht
authors.forename: Markus
authors.initials: M
authors.lastname: Meissner
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology*; D020868:Gene Silencing; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D015800:Protozoan Proteins / Q000235:genetics*; D014122:Toxoplasma / Q000235:genetics*
chemical_list: D015800:Protozoan Proteins