Pancreatic islet immunoreactivity to the Reg protein INGAP.
The Reg-related protein family member INGAP (islet neogenesis-associated protein) is a pleiotropic factor enhancing islet neogenesis, neurite growth, beta-cell protection, and beta-cell function. Using an antibody to the N-termini of INGAP, we have identified that immunoreactivity to INGAP localized to the pancreatic endocrine cells in mouse. INGAP- and insulin-immunoreactive cells are mutually exclusive, with INGAP-immunoreactive cells being preserved after streptozotocin-mediated destruction of beta-cells. Glucagon- and INGAP-immunoreactive cells colocalize, although respective antigen expression occurs in different intracellular locations. These data suggest that INGAP-immunoreactive cells include alpha-cells; however, detection of single INGAP-immunoreactive/glucagon-negative cells indicates that this may not be exclusive. In addition to mouse, detection of islet endocrine cells that were INGAP immunoreactive/glucagon immunoreactive/insulin negative was also observed in islets from human, monkey, and rat. These findings reveal that INGAP and/or related group 3 Reg proteins have a conserved expression in the pancreatic islet.
Taylor-Fishwick
David A
DA
Department of Medicine,, Eastern Virginia Medical School, 855 W. Brambleton Ave., Norfolk, VA, USA. Taylord@evms.edu
Bowman
Angela
A
Korngiebel-Rosique
Maricarmen
M
Vinik
Aaron I
AI
D000818:Animals; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002460:Cell Line; D003429:Cross Reactions; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D050416:Glucagon-Secreting Cells / Q000378:metabolism; D000882:Haplorhini; D006801:Humans; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism*; D037181:Lectins, C-Type / Q000378:metabolism*; D008297:Male; D051379:Mice; D000073718:Pancreatitis-Associated Proteins; D021381:Protein Transport; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D013045:Species Specificity
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D007328:Insulin; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins; C000612141:REG3A protein, human; D011994:Recombinant Proteins; C490253:Reg3b protein, mouse; C000612144:Reg3b protein, rat; D005934:Glucagon