title: RANTES gene promoter polymorphisms are associated with bronchial hyperresponsiveness in Korean children with asthma.
abstract: Regulated upon activation in normal T cells, expressed, and secreted (RANTES) protein is abundantly expressed during atopic asthma, suggesting that it is an important mediator of this disease. The aim of this study was to evaluate the possible role of RANTES promoter polymorphisms in children with asthma. We genotyped 271 children with atopic asthma, 55 children with nonatopic asthma, and 253 control children for allelic determinants at two polymorphic sites in the promoter region at positions -403G>A and -28C>G by restriction fragment length polymorphism methods. There was no significant difference in genotype and allele frequencies of the RANTES -403G>A and -28C>G polymorphisms when the atopic asthma, nonatopic asthma, and control groups were compared. However, atopic asthmatic patients who were homozygous GG for the RANTES -28C>G tended to have lower PC20 methacholine than those carrying the wild genotype. In addition, a significantly lower PC20 was demonstrated for the homozygous haplotype -403A/-28G in asthmatic children. The polymorphisms within the RANTES promoter may have a disease-modifying effect in Korean children with asthma.
authors.affiliation: Department of Pediatrics and Institute of Allergy, Yonsei University College of Medicine, Brain Korea 21 Project for Medical Sciences, Seoul, Korea.
authors.forename: Myung Hyun
authors.initials: MH
authors.lastname: Sohn
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Kyung-Won
authors.initials: KW
authors.lastname: Kim
authors.forename: Hye Mi
authors.initials: HM
authors.lastname: Jee
authors.forename: Hae-Sim
authors.initials: HS
authors.lastname: Park
authors.forename: Kyu-Earn
authors.initials: KE
authors.lastname: Kim
mesh_terms: D000293:Adolescent; D001249:Asthma / Q000175:diagnosis / Q000235:genetics* / Q000503:physiopathology; D016535:Bronchial Hyperreactivity / Q000175:diagnosis / Q000235:genetics* / Q000503:physiopathology; D001985:Bronchial Provocation Tests; D016085:Bronchoconstrictor Agents; D016022:Case-Control Studies; D018946:Chemokine CCL5 / Q000235:genetics*; D002648:Child; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006720:Homozygote; D006801:Humans; D007723:Korea; D008297:Male; D016210:Methacholine Chloride; D010641:Phenotype; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012307:Risk Factors
chemical_list: D016085:Bronchoconstrictor Agents; C483024:CCL5 protein, human; D018946:Chemokine CCL5; D016210:Methacholine Chloride