title: In vivo methods for the assessment of topical drug bioavailability.
abstract: This paper reviews some current methods for the in vivo assessment of local cutaneous bioavailability in humans after topical drug application. After an introduction discussing the importance of local drug bioavailability assessment and the limitations of model-based predictions, the focus turns to the relevance of experimental studies. The available techniques are then reviewed in detail, with particular emphasis on the tape stripping and microdialysis methodologies. Other less developed techniques, including the skin biopsy, suction blister, follicle removal and confocal Raman spectroscopy techniques are also described.
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva, Geneva 4, Switzerland.
authors.forename: Christophe
authors.initials: C
authors.lastname: Herkenne
authors.forename: Ingo
authors.initials: I
authors.lastname: Alberti
authors.forename: Aarti
authors.initials: A
authors.lastname: Naik
authors.forename: Yogeshvar N
authors.initials: YN
authors.lastname: Kalia
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Mathy
authors.forename: Véronique
authors.initials: V
authors.lastname: Préat
authors.forename: Richard H
authors.initials: RH
authors.lastname: Guy
mesh_terms: D000287:Administration, Topical; D000465:Algorithms; D001682:Biological Availability; D006801:Humans; D017551:Microdialysis; D018613:Microscopy, Confocal; D004364:Pharmaceutical Preparations / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism*; D010599:Pharmacokinetics; D012867:Skin / Q000378:metabolism; D012869:Skin Absorption
chemical_list: D004364:Pharmaceutical Preparations