title: [Expression of K18, Ser-33 and Ser-52 phosphorylated K18 in HBV infected human liver disease and its significance].
abstract: OBJECTIVE
  To investigate the expression of K18, Ser-33 and Ser-52 phosphorylated K18 in HBV infected human liver disease and its significance.
  
  
  METHODS
  The expression and localization of K18 and Ser-33, Ser-52 phosphorylated K18 in healthy liver tissue, in liver tissues of patients with post-HBV infection cirrhosis and severe chronic hepatitis were detected by histochemistry.
  
  
  RESULTS
  K18, Ser-33 and Ser-52 phosphorylated K18 were expressed in normal liver cells, in liver tissues of cirrhosis patients and severe chronic hepatitis cases. The expression of K18 in the liver cells from the 3 different sources had no significant difference in levels. Ser-33 and Ser-52 phosphorylated K18 were expressed in normal liver cells, in liver tissues of cirrhosis patients chronicity HBV hepatitis and severe chronic hepatitis cases. Ser-33 and Ser-52 located around cytoplasmic membrane, diffused into cytoplasm and expressed at a higher levels in cirrhosis and severe chronic hepatitis.
  
  
  CONCLUSIONS
  The expression levels of Ser-33 and Ser-52 phosphorylated K18 increased along with the progression of HBV infected human liver disease. The phosphorylation of K18 could be a marker of progression of HBV infected human liver disease.
authors.affiliation: Department of Infectious Diseases, Beijing Youan Hospital, Capital University of Medical Sciences, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shi
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Lian-Chun
authors.initials: LC
authors.lastname: Liang
authors.forename: Ya-Song
authors.initials: YS
authors.lastname: Wu
authors.forename: Yu-Sen
authors.initials: YS
authors.lastname: Zhou
authors.forename: Xin-Yue
authors.initials: XY
authors.lastname: Chen
authors.forename: De-Xi
authors.initials: DX
authors.lastname: Chen
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
mesh_terms: D006509:Hepatitis B / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D053538:Keratin-18 / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000378:metabolism / Q000473:pathology / Q000821:virology; D008107:Liver Diseases / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D010766:Phosphorylation; D012694:Serine / Q000378:metabolism
chemical_list: D053538:Keratin-18; D012694:Serine