One-step photochemical introduction of nanopatterned protein-binding functionalities to oligo(ethylene glycol)-terminated self-assembled monolayers.
Exposure of oligo(ethylene glycol) (OEG)-terminated self-assembled monolayers (SAMs) to UV light leads to the formation of aldehyde groups, leading to a simple one-step method for the introduction of reactive functional groups to protein-resistant surfaces. X-ray photoelectron spectroscopy has been used to demonstrate binding of amines to the modified surfaces, while surface plasmon resonance has shown that proteins are covalently bound. Modified OEG monolayers bind streptavidin at least as well as N-hydroxysuccinimidyl ester functionalized monolayers. Micrometer and nanometer-scale patterns are conveniently fabricated by exposing the monolayers using, respectively, a mask and a scanning near-field optical microscope.
Ducker
Robert E
RE
Department of Chemistry, University of Sheffield, Brook Hill, Sheffield S3 7HF, United Kingdom.
Janusz
Stefan
S
Sun
Shuqing
S
Leggett
Graham J
GJ
D000588:Amines / Q000737:chemistry; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010777:Photochemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D013057:Spectrum Analysis; D020349:Surface Plasmon Resonance
D000588:Amines; D011506:Proteins; D011092:Polyethylene Glycols