[Development and characterization of monoclonal antibodies against VP1 protein of AsiaI type foot-and-mouth virus].
OBJECTIVE
To prepare the monoclonal antibodies against VP1 protein of type AsiaI foot-and mouth disease virus (FMDV) and identify the characterization.


METHODS
Three cell of hybridization that strains secreted monoclonal antibody(mAb) against type AsiaI FMDV were produced by fusing mouse myeloma cells (Sp2/0) with spleen cells from BALB/c immunized with the purified recombinant VP1 protein.


RESULTS
The three hybridized cell lines reacted with cattle type AsiaI FMDV, the titres of ascetic fluids of the three mAbs ranged from 1:10(5) to 1:10(6) indirect ELISA showed. Among the three mAbs,1B8 belonged to IgG1, 5E1 and 5E2 pertained to IgG2a. Besides, the three strain antibodies stably excreted antibodies and reacted with type AsiaI FMDV.


CONCLUSIONS
VP1 protein of FMDV could replace FMDV can be use to replace FMDV to prepare mAbs.
Lin
Tong
T
Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Chang
Hui-Yun
HY
DU
Hui-Fen
HF
Shao
Jun-Jun
JJ
DU
Jun-Zheng
JZ
Cong
Guo-Zheng
GZ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D000918:Antibody Specificity; D036022:Capsid Proteins / Q000276:immunology*; D002460:Cell Line; D002875:Chromosomes / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D029544:Foot-and-Mouth Disease Virus / Q000737:chemistry / Q000276:immunology; D006825:Hybridomas / Q000166:cytology / Q000378:metabolism; D050440:Immunoglobulin Subunits / Q000032:analysis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; D050440:Immunoglobulin Subunits; C442501:VP1 protein, Foot-and-mouth disease virus