title: [Research on isolation and purification of total iridoid glycosides from herbal Lamiophlomis rotata].
abstract: OBJECTIVE
  Enriching the hemostatic active part total iridoid glycosides from the aqueous extract of herbal Lamiophlomis rotata.
  
  
  METHODS
  First derivative spectrophotometry was used as the detection method and the content of total iridoid glycosides was used as tracking target. The abilities of absorption and anti-absorption of ten different types of macroreticular resin were compared, after being used to absorb the iridoid glycosides from aqueous extract of herbal L. rotata. The best purification process was optimized.
  
  
  RESULTS
  With the optimized purification process, the content of total iridoid glycosides was 67.53%.
  
  
  CONCLUSIONS
  XDA-1 macroreticular resin has good ability of absorption and anti-absorption for iridoid glycosides and 50% ethanol was the best solvent for anti-absorption. The macroreticular resin could be reused.
authors.affiliation: Department of Pharmacy, Lanzhou General Hospital of PLA, Lanzhou 730050, China. lm730530xing@yahoo.com.cn
authors.forename: Mao-xing
authors.initials: MX
authors.lastname: Li
authors.forename: Zheng-ping
authors.initials: ZP
authors.lastname: Jia
authors.forename: Zhi-de
authors.initials: ZD
authors.lastname: Hu
authors.forename: Ru-xue
authors.initials: RX
authors.lastname: Zhang
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Ren
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
mesh_terms: D006027:Glycosides / Q000302:isolation & purification*; D039823:Iridoids / Q000302:isolation & purification*; D019686:Lamiaceae / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012117:Resins, Synthetic; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D006027:Glycosides; D039823:Iridoids; D012117:Resins, Synthetic