The yeast acylglycerol acyltransferase LCA1 is a key component of Lands cycle for phosphatidylcholine turnover.
Cellular phospholipids undergo deacylation and reacylation through a process known as Lands cycle. In this report, we provide evidence demonstrating that yeast YOR175c, herein designated as LCA1, encodes a key component of the Lands cycle, the acyl-CoA: lysophosphatidylcholine acyltransferase (LPCAT). Deletion of LCA1 resulted in a drastic reduction in LPCAT activity, while over expression led to a several fold increase in enzyme activity. We further show that disruption of LCA1 caused an enhanced production of glycerophosphorylcholine, a product of phosphatidylcholine (PC) deacylation and that the lysophosphatidic acid acyltransferase SLC1 was not involved in this process. Identification of LCA1 provides an essential molecular tool for further study of Lands cycle in PC turnover.
Chen
Qilin
Q
Plant Biotechnology Institute, National Research Council Canada, 110 Gymnasium Place, Saskatoon, SK, Canada.
Kazachkov
Michael
M
Zheng
Zhifu
Z
Zou
Jitao
J
D008243:1-Acylglycerophosphocholine O-Acyltransferase / Q000235:genetics / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004791:Enzyme Inhibitors / Q000494:pharmacology; D017353:Gene Deletion; D007700:Kinetics; D010713:Phosphatidylcholines / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000378:metabolism; D054645:Protein Phosphatase 1; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013379:Substrate Specificity; D015032:Zinc / Q000494:pharmacology
D004791:Enzyme Inhibitors; C073060:GAC1 protein, S cerevisiae; C029271:O-deacetyl platelet activating factor; D010713:Phosphatidylcholines; D010972:Platelet Activating Factor; D029701:Saccharomyces cerevisiae Proteins; D008243:1-Acylglycerophosphocholine O-Acyltransferase; C525807:ALE1 protein, S cerevisiae; D054645:Protein Phosphatase 1; D015032:Zinc