[Multicolor FISH analysis of rDNA and telomere on spin-ach].
In this study, multicolor FISH analysis on metaphase chromosomes of spinach with biotin-labeled 25S rDNA, DIG-labeled telomere sequences and biotin-labeled and DIG-labeled 5S rDNA was performed. There were six 25S rDNA loci, which were located on the satellites of the third, the fifth and the sixth chromosomes, four 5S rDNA loci, which were located on the long arms of the third and the fifth chromosomes. The telomere loci were located on the end of the sixth chromosome and also on both the end and centromeric regions of other chromosomes. This study is an important complement to both traditional karyotype analysis and FISH karyotype analysis in spinach.
Lan
Tian-Ying
TY
College of Life Sciences, Nankai University, Tianjin 300071, China. lan_ty1982@yahoo.com.cn
Liu
Bo
B
Dong
Feng-Ping
FP
Chen
Rui-Yang
RY
Li
Xiu-Lan
XL
Chen
Cheng-Bin
CB
D032461:Chromosomes, Plant; D018744:DNA, Plant / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007621:Karyotyping; D012335:RNA, Ribosomal / Q000032:analysis / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000032:analysis / Q000235:genetics; D018724:Spinacia oleracea / Q000235:genetics*; D016615:Telomere / Q000737:chemistry / Q000235:genetics*
D018744:DNA, Plant; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S