title: In vivo tracing of neural tracts in the intact and injured spinal cord of marmosets by diffusion tensor tractography.
abstract: In spinal cord injury, axonal disruption results in motor and sensory function impairment. The evaluation of axonal fibers is essential to assess the severity of injury and efficacy of any treatment protocol, but conventional methods such as tracer injection in brain parenchyma are highly invasive and require histological evaluation, precluding clinical applications. Previous advances in magnetic resonance imaging technology have led to the development of diffusion tensor tractography (DTT) as a potential modality to perform in vivo tracing of axonal fibers. The properties and clinical applications of DTT in the brain have been reported, but technical difficulties have limited DTT studies of the spinal cord. In this study, we report the effective use of DTT to visualize both intact and surgically disrupted spinal long tracts in adult common marmosets. To verify the feasibility of spinal cord DTT, we first performed DTT of postmortem marmosets. DTT clearly illustrated spinal projections such as the corticospinal tract and afferent fibers in control animals, and depicted the severed long tracts in the injured animals. Histology of the spinal cords in both control and injured groups were consistent with DTT findings, verifying the accuracy of DTT. We also conducted DTT in live marmosets and demonstrated that DTT can be performed in live animals to reveal in vivo nerve fiber tracing images, providing an essential tool to evaluate axonal conditions in the injured spinal cord. Taken together, these findings demonstrate the feasibility of applying DTT to preclinical and clinical studies of spinal cord injury.
authors.affiliation: Department of Orthopaedic Surgery, Keio University School of Medicine, Shinjuku, Tokyo 160-8582, Japan.
authors.forename: Kanehiro
authors.initials: K
authors.lastname: Fujiyoshi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamada
authors.forename: Masaya
authors.initials: M
authors.lastname: Nakamura
authors.forename: Junichi
authors.initials: J
authors.lastname: Yamane
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Katoh
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kitamura
authors.forename: Kenji
authors.initials: K
authors.lastname: Kawai
authors.forename: Seiji
authors.initials: S
authors.lastname: Okada
authors.forename: Suketaka
authors.initials: S
authors.lastname: Momoshima
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Toyama
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
mesh_terms: D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism; D002144:Callithrix; D038524:Diffusion Magnetic Resonance Imaging; D004195:Disease Models, Animal; D005260:Female; D007839:Functional Laterality; D008279:Magnetic Resonance Imaging; D011712:Pyramidal Tracts / Q000473:pathology; D013116:Spinal Cord / Q000473:pathology*; D013119:Spinal Cord Injuries / Q000473:pathology*
chemical_list: D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2