Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.).
Peroxidase (POD) and superoxide dismutase (SOD) enzyme activities were analyzed in non-regenerative transformed embryogenic lines of alfalfa (Medicago sativa L.) carrying wound-inducible oryzacystatin I (OC-I), wound-inducible oryzacystatin I antisense (OC-Ias), or hygromycin phosphotransferase (hpt) genes. All of the transformed lines analyzed had elevated levels of all POD isoforms. Three POD isoforms with pI values of approximately 4.5, 4.8, and 8.4, and one additional pair of isoforms with a pI value of approximately 8.8 were separated from tissue extracts of all transgenic lines. Isoelectrofocusing patterns revealed the induction of one isoform of SOD with a pI of about 5.6 in all transgenic lines compared with non-transformed embryogenic tissue. These results indicate that the process of transformation may disrupt redox homeostasis in alfalfa tissues.
Platisa
Jelena
J
Center for Multidisciplinary Studies, University of Belgrade, Kneza Viseslava 1a, 11030 Belgrade, Serbia.
Veljović-Jovanović
Sonja
S
Kukavica
Biljana
B
Vinterhalter
Branka
B
Smigocki
Ann
A
Ninković
Slavica
S
D018533:Biomass; D004351:Drug Resistance; D047108:Embryonic Development; D004790:Enzyme Induction; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000378:metabolism; D000455:Medicago sativa / Q000196:embryology* / Q000201:enzymology* / Q000235:genetics; D010544:Peroxidases / Q000096:biosynthesis*; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D011748:Pyrogallol / Q000378:metabolism; D016372:RNA, Antisense; D012984:Software; D013379:Substrate Specificity; D013482:Superoxide Dismutase / Q000096:biosynthesis*; D014020:Tissue Extracts; D014170:Transformation, Genetic
D007527:Isoenzymes; D010940:Plant Proteins; D016372:RNA, Antisense; D014020:Tissue Extracts; D011748:Pyrogallol; D010544:Peroxidases; D013482:Superoxide Dismutase