Birth of a healthy boy after fertilization of cryopreserved oocytes with cryopreserved testicular spermatozoa from a man with nonmosaic Klinefelter syndrome.
OBJECTIVE
To report the first birth with frozen spermatozoa and frozen oocytes in a case of nonmosaic Klinefelter syndrome.


METHODS
Case report.


METHODS
Private IVF clinics and a university hospital.


METHODS
A 32-year-old woman and a 33-year-old man.


METHODS
Testicular sperm extraction and cryopreservation, oocyte recovery and cryopreservation, intracytoplasmic sperm injection.


METHODS
Assessment of fertilization and chromosomal analysis of newborn child.


RESULTS
Three oocytes were injected and one embryo was obtained, resulting in the birth of a normal (46,XY) boy.


CONCLUSIONS
Cryopreserved spermatozoa from a patient with nonmosaic Klinefelter syndrome can form normal embryos even with cryopreserved oocytes.
Greco
Ermanno
E
Center for Reproductive Medicine, European Hospital, Rome, Italy.
Iacobelli
Marcello
M
Rienzi
Laura
L
Fabris
Giuseppe F Menchini
GF
Tesorio
Nicola
N
Tesarik
Jan
J
D000328:Adult; D053713:Azoospermia / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007713:Klinefelter Syndrome / Q000150:complications* / Q000473:pathology; D050498:Live Birth; D008297:Male; D054315:Oocyte Retrieval; D009865:Oocytes; D010062:Ovulation Induction; D011247:Pregnancy; D012662:Semen Preservation; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa; D013737:Testis / Q000473:pathology*; D016896:Treatment Outcome