title: Gene expression in the rectus abdominus muscle of patients with and without pelvic organ prolapse.
abstract: OBJECTIVE
  The objective of the study was to compare gene expression in a group of actin and myosin-related proteins in the rectus muscle of 15 patients with pelvic organ prolapse and 13 controls.
  
  
  METHODS
  Six genes previously identified by microarray GeneChip analysis were examined using real-time quantitative reverse transcriptase-polymerase chain reaction analysis, including 2 genes showing differential expression in pubococcygeus muscle. Samples and controls were run in triplicate in multiplexed wells, and levels of gene expression were analyzed using the comparative critical threshold method.
  
  
  RESULTS
  One gene, MYH3, was 3.2 times overexpressed in patients with prolapse (P = .032), but no significant differences in expression were seen for the other genes examined. An age-matched subset of 9 patients and controls showed that MYH3 gene expression was no longer significantly different (P = .058).
  
  
  CONCLUSIONS
  Differential messenger ribonucleic acid levels of actin and myosin-related genes in patients with pelvic organ prolapse and controls may be limited to skeletal muscle from the pelvic floor.
authors.affiliation: Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Obstetrics and Gynecology, The Ohio State University Medical Center, Columbus, OH, USA.
authors.forename: Andrew F
authors.initials: AF
authors.lastname: Hundley
authors.forename: Lingwen
authors.initials: L
authors.lastname: Yuan
authors.forename: Anthony G
authors.initials: AG
authors.lastname: Visco
mesh_terms: D000199:Actins / Q000235:genetics*; D016022:Case-Control Studies; D003598:Cytoskeletal Proteins / Q000235:genetics; D017931:DNA Primers; D005260:Female; D005786:Gene Expression Regulation; D020022:Genetic Predisposition to Disease; D006801:Humans; D008875:Middle Aged; D009218:Myosins / Q000235:genetics*; D012313:RNA / Q000032:analysis; D017568:Rectus Abdominis / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014596:Uterine Prolapse / Q000235:genetics*; D044465:White People / Q000235:genetics
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; D017931:DNA Primers; C510594:MYH3 polypeptide, human; D012313:RNA; D009218:Myosins