An antidiuretic peptide (Tenmo-ADFb) with kinin-like diuretic activity on Malpighian tubules of the house cricket, Acheta domesticus (L.).
Acheta domesticus is reported to have an antidiuretic hormone that reduces Malpighian tubule secretion. Identified peptides known to work in this way (Tenmo-ADFa and ADFb, and Manse-CAP(2b)) were tested as candidates for the unidentified hormone, along with their second messenger, cyclic GMP. Only Tenmo-ADFb was active, but was diuretic, as was 8-bromo cyclic GMP. The activity of Tenmo-ADFb is comparable to that of the cricket kinin neuropeptide, Achdo-KII, but it is much less potent. Its activity was unaffected by deleting either the six N-terminal residues or the C-terminal phenylalanine. At high concentrations, tubule secretion is doubled by Tenmo-ADFb and Achdo-KII, but their actions are non-additive, suggesting they have a similar mode of action. Both stimulate a non-selective KCl and NaCl diuresis, which is consistent with the opening of a transepithelial Cl(-) conductance. In support of this, the diuretic response to Tenmo-ADFb and Achdo-KII is prevented by a ten-fold reduction in bathing fluid chloride concentration, and both peptides cause the lumen-positive transepithelial voltage to collapse. The Cl(-) conductance pathway appears likely to be transcellular, because the Cl(-) channel blocker DPC reduces both basal and peptide-stimulated rates of secretion. The effects of 8-bromo cyclic GMP on transepithelial voltage and composition of the secreted fluid are markedly different from those of Tenmo-ADFb. This is the first report of the antidiuretic factor Tenmo-ADFb stimulating tubule secretion. Although the actions of Tenmo-ADFb are indistinguishable from those of Achdo-KII, it is unlikely to act at a kinin receptor, because the core sequence (residues 7-12) lacks the Phe and Trp residues that are critical for kinin activity.
Coast
Geoffrey M
GM
School of Biological and Chemical Sciences, Birkbeck, University of London, Malet Street, London WC1E 7HX, UK. g.coast@bbk.ac.uk
Nachman
Ronald J
RJ
Schooley
David A
DA
D000595:Amino Acid Sequence; D000818:Animals; D050034:Antidiuretic Agents / Q000494:pharmacology*; D001826:Body Fluids / Q000187:drug effects / Q000378:metabolism; D002412:Cations; D018118:Chloride Channels / Q000037:antagonists & inhibitors; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology; D004232:Diuretics / Q000494:pharmacology*; D004357:Drug Synergism; D004594:Electrophysiology; D004848:Epithelium / Q000187:drug effects; D005260:Female; D041981:Gastrointestinal Tract / Q000187:drug effects; D006135:Gryllidae / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007705:Kinins / Q000494:pharmacology*; D008317:Malpighian Tubules / Q000187:drug effects*; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013997:Time Factors
D050034:Antidiuretic Agents; D002412:Cations; D018118:Chloride Channels; D004232:Diuretics; D007705:Kinins; D010455:Peptides; C016276:8-bromocyclic GMP; D006152:Cyclic GMP