[Inhibition of advanced glycation by flavonoids. A nutritional implication for preventing diabetes complications?].
Advanced glycation of collagens contributes to development of micro- and macrovascular complications in diabetes. Since flavonoids are potent natural antioxidants, it was interesting to examine their effect on the formation of a cross-linking advanced glycation endproduct, pentosidine, in collagen incubated with glucose. Monomeric flavonoids (25 and 250 microM) markedly reduced pentosidine/hydroxyproline values in a concentration- and structure-dependent manner. Procyanidin oligomers from grape seed were more active than pine bark procyanidin oligomers. Oligomers are known to be cleaved into monomers in the gastric milieu and monomeric flavonoids to be absorbed and recovered at micromolar concentrations (with a long plasmatic half-life) in extracellular fluids, in contact with collagens. In conclusion, flavonoids are very potent inhibitors of pentosidine formation in collagens, active at micromolar concentrations; these concentrations might be achieved in plasma of diabetic patients after oral intake of flavonoids.
Urios
Paul
P
Département de Biochimie, Faculté de Médecine & Laboratoire de Pharmacologie, Faculté de Pharmacie, Université René Descartes, Paris, France. paul.urios@bch.aphp.fr
Grigorova-Borsos
Anne-Marie
AM
Peyroux
Jacques
J
Sternberg
Michel
M
D001120:Arginine / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors; D048909:Diabetes Complications / Q000517:prevention & control*; D019587:Dietary Supplements; D005419:Flavonoids / Q000737:chemistry / Q000627:therapeutic use*; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors*; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors
D005419:Flavonoids; D017127:Glycation End Products, Advanced; D001120:Arginine; C062187:pentosidine; D008239:Lysine