Langerhans cells are required for efficient presentation of topically applied hapten to T cells.
Dendritic cells (DC) play a pivotal role in the control of T cell immunity due to their ability to stimulate naive T cells and direct effector function. Murine and human DC are composed of a number of phenotypically, and probably developmentally, distinct subsets, which may play unique roles in the initiation and regulation of T cell responses. The skin is populated by at least two subsets of DC: Langerhans cells (LC), which form a contiguous network throughout the epidermis, and dermal DC. LC have classically been thought vital to initiate T cell responses to cutaneous Ags. However, recent data have highlighted the importance of dermal DC in cutaneous immunity, and the requirement for LC has become unclear. To define the relative roles of LC and dermal DC, we and others generated mouse models in which LC were specifically depleted in vivo. Unexpectedly, these studies yielded conflicting data as to the role of LC in cutaneous contact hypersensitivity (CHS). Extending our initial finding, we demonstrate that topical Ag is inefficiently transported to draining lymph nodes in the absence of LC, resulting in suboptimal priming of T cells and reduced CHS. However, dermal DC may also prime cutaneous T cell responses, suggesting redundancy between the two different skin DC subsets in this model.
Bennett
Clare L
CL
Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Noordegraaf
Madelon
M
Martina
Cerithsa A E
CA
Clausen
Björn E
BE
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D001693:Biological Transport, Active / Q000276:immunology; D003877:Dermatitis, Contact / Q000276:immunology*; D020405:Dermis / Q000276:immunology; D004817:Epidermis / Q000276:immunology; D006241:Haptens / Q000276:immunology*; D006801:Humans; D007801:Langerhans Cells / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D018448:Models, Immunological; D013601:T-Lymphocytes
D006241:Haptens