Evaluation of anti-inflammatory activity of alcoholic extract from Viola tricolor.
OBJECTIVE
Evaluation of anti-inflammatory actions of Viola tricolor aerial parts tincture.


METHODS
The anti-inflammatory activity of the tincture from Viola tricolor aerial parts was tested in acute inflammation induced with oil of turpentine (i.m. 0.6 ml/100 g b.w.) in male Wistar rats. The results were compared with those from a positive control group with experimental inflammation and with those of a group treated with diclofenac (30 mg/100 g b.w.). We tested the effects by measuring total leukocyte count and differential leukocyte count, a test of in vitro phagocytosis and by the evaluation of nitric oxide (NO) synthesis measuring the metabolites (nitrites and nitrates).


RESULTS
Viola tricolor extract (50 mg tincture/100 g b.w.) significantly reduced polymorphonuclear leukocytes and monocytes percentages and the activation of circulating phagocytes. Nitric oxide synthesis had a slight decrease.


CONCLUSIONS
Viola tricolor extract had anti-inflammatory effect on bone marrow acute phase response.
Toiu
Anca
A
Department of Botany, School of Pharmacy,"Iuliu Haţieganu" University of Medicine and Pharmacy Cluj-Napoca.
Pârvu
Alina Elena
AE
Oniga
Ilioara
I
Tămaş
M
M
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D004008:Diclofenac / Q000627:therapeutic use; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D007509:Irritants / Q000009:adverse effects; D007958:Leukocyte Count; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D032413:Viola
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007509:Irritants; D010936:Plant Extracts; D004008:Diclofenac; D009569:Nitric Oxide