[Establishment, optimization and application of a screening method for immunoregulatory oligodeoxynucleotides].
OBJECTIVE
To establish a set of reasonable and convenient experimental system to provide a screening method for the development of novel immunoregulatory oligodeoxynucleotides.


METHODS
The human PBMCs were stimulated by CpG ODN and/or immunoregulatory ODN. The cell proliferation and anti-viral activity of the supernatant induced by CpG ODN were examined by thymidine incorporation and anti-viral bioassay to evaluate the immunoregulatory activity of candidate ODN. The experimental conditions were also optimized.


RESULTS
A screening method on which A151, a positive immunoregulatory ODN, inhibited the proliferation and anti-viral activity of the CpG ODN-induced human PBMCs was successfully established.


CONCLUSIONS
The successful establishment of CpG ODN based screening method lays the foundations for further development of novel immunoregulatory oligodeoxynucleotides.
Hu
Da-Li
DL
Department of Immunology, Basic Medical College of Jilin University, Changchun 130021, China.
Zhang
Xue-Song
XS
Wang
Hua-Ying
HY
Sun
Ran
R
Ren
Ji-Ling
JL
Wang
Li-Ying
LY
Yu
Yong-Li
YL
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D049109:Cell Proliferation; D018899:CpG Islands / Q000235:genetics; D004306:Dose-Response Relationship, Immunologic; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000032:analysis* / Q000235:genetics / Q000276:immunology*; D013997:Time Factors; D018116:Vesiculovirus / Q000276:immunology
D009838:Oligodeoxyribonucleotides