title: Second harmonic generation imaging microscopy studies of osteogenesis imperfecta.
abstract: We have used quantitative second harmonic generation (SHG) imaging microscopy to investigate the collagen matrix organization in the oim mouse model for human osteogenesis imperfecta (OI). OI is a heritable disease in which the type I collagen fibrils are either abnormally organized or small, resulting in a clinical presentation of recurrent bone fractures and other pathologies related to collagen-comprised tissues. Exploiting the exquisite sensitivity of SHG to supramolecular assembly, we investigated whether this approach can be utilized to differentiate normal and oim tissues. By comparing SHG intensity, fibrillar morphology, polarization anisotropy, and signal directionality, we show that statistically different results are obtained for the wild type (WT) and disease states in bone, tendon, and skin. All these optical signatures are consistent with the collagen matrix in the oim tissues being more disordered, and these results are further consistent with the known weaker mechanical properties of the oim mouse. While the current work shows the ability of SHG to differentiate normal and diseased states in a mouse model, we suggest that our results provide a framework for using SHG as a clinical diagnostic tool for human OI. We further suggest that the SHG metrics described could be applied to other connective tissue disorders that are characterized by abnormal collagen assembly.
authors.affiliation: University of Connecticut Health Center, Department of Cell Biology, Center for Cellular Analysis and Modeling, Farmington, Connecticut 06030, USA.
authors.forename: Oleg
authors.initials: O
authors.lastname: Nadiarnykh
authors.forename: Sergey
authors.initials: S
authors.lastname: Plotnikov
authors.forename: William A
authors.initials: WA
authors.lastname: Mohler
authors.forename: Ivo
authors.initials: I
authors.lastname: Kalajzic
authors.forename: Deborah
authors.initials: D
authors.lastname: Redford-Badwal
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Campagnola
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000648:ultrastructure*; D005269:Femur / Q000648:ultrastructure*; D007089:Image Enhancement / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal / Q000379:methods*; D010013:Osteogenesis Imperfecta / Q000648:ultrastructure*
chemical_list: D024042:Collagen Type I