Role of calcium and vesicle-docking proteins in remobilising dormant neuromuscular junctions in desert frogs.
Despite prolonged immobility the desert frog, Cyclorana alboguttata, suffers little impairment in muscle function. To determine compensatory mechanisms at neuromuscular junctions, transmitter release was examined along primary terminals in C. alboguttata iliofibularis muscle. Using extracellular recording we found the amplitudes of evoked endplate currents were significantly smaller in dormant frogs. In active frogs we identified two negatively sloping proximal-distal gradients of transmitter frequency and quantal content; a shallow proximal-distal gradient with low probability of transmitter release (<0.2) and a second much steeper proximal-distal gradient for quantal content with high probability release sites (>0.6). During aestivation, only a shallow gradient was identified. The high probability release sites in control frogs were inhibited during aestivation by a mechanism that could be reversed by (1) increasing the extracellular calcium concentration, and (2) increasing the frequency of stimulation. This suggests that transmitter vesicles are available during aestivation but not released. We quantified expression of messenger RNA transcripts coding for the transmitter vesicle-docking proteins synaptotagmin 1, syntaxin 1B and UNC-13. All three were rare transcripts maintained at control values during aestivation. Neuromuscular remobilisation after dormancy in C. alboguttata is more likely a product of rapidly reversible physiologic mechanisms than reorganisations of the neuromuscular transcriptome.
Lavidis
Nickolas A
NA
School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia.
Hudson
Nicholas J
NJ
Choy
Peng T
PT
Lehnert
Sigrid A
SA
Franklin
Craig E
CE
D000222:Adaptation, Physiological; D000818:Animals; D001001:Anura / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D003889:Desert Climate; D004956:Estivation / Q000502:physiology*; D005260:Female; D008297:Male; D016555:Matched-Pair Analysis; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009045:Motor Endplate / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D050479:Neurotransmitter Transport Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D050863:Synaptotagmin I / Q000235:genetics / Q000378:metabolism; D050827:Syntaxin 1 / Q000235:genetics / Q000378:metabolism; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050479:Neurotransmitter Transport Proteins; D012333:RNA, Messenger; D050863:Synaptotagmin I; D050827:Syntaxin 1; D033921:Vesicular Transport Proteins; D002118:Calcium