An EntD-like phosphopantetheinyl transferase gene from Photobacterium profundum SS9 complements pfa genes of Moritella marina strain MP-1 involved in biosynthesis of docosahexaenoic acid.
The EntD-like phosphopantetheinyl transferase (PPTase) gene, cloned from the eicosapentaenoic acid-producing bacterium Photobacterium profundum strain SS9, has an ORF of 690 bp encoding a 230-amino acid protein. When this PPTase gene was expressed in Escherichia coli with pfaA, pfaB, pfaC and pfaD derived from Moritella marina MP-1, which were four of five essential genes for biosynthesis of docosahexaenoic acid (DHA), the DHA production of the recombinant was 2% (w/w) of total fatty acids. This is the first report showing that the EntD-like PPTase is involved in producing n-3 polyunsaturated fatty acids.
Sugihara
Shinji
S
Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo, Japan.
Orikasa
Yoshitake
Y
Okuyama
Hidetoshi
H
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D034221:Moritella / Q000235:genetics* / Q000378:metabolism; D010776:Photobacterium / Q000201:enzymology / Q000235:genetics*; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; C082662:phosphopantetheinyl transferase; D004281:Docosahexaenoic Acids; D017855:Transferases (Other Substituted Phosphate Groups)