title: Sexual development in Cryptococcus neoformans requires CLP1, a target of the homeodomain transcription factors Sxi1alpha and Sxi2a.
abstract: Sexual development in the human fungal pathogen Cryptococcus neoformans is a multistep process that results in the formation of spores, the likely infectious particles. A critical step in this developmental process is the transition from bud-form growth to filamentous growth. This transition is controlled by the homeodomain transcription factors Sxi1alpha and Sxi2a, whose targets are largely unknown. Here we describe the discovery of a gene, CLP1, that is regulated by Sxi1alpha and Sxi2a and is essential for sexual development. In vitro binding studies also show that the CLP1 promoter is bound directly by Sxi1alpha and Sxi2a. The deletion of CLP1 leads to a block in sexual development after cell fusion but before filament formation, and cells without CLP1 are unable to grow vegetatively after cell fusion. Our findings lead to a model in which CLP1 is a downstream target of the Sxi proteins that functions to promote growth after mating and to establish the filamentous state, a critical step in the production of spores.
authors.affiliation: University of Wisconsin, Madison, 587 Medical Science Center, 1300 University Avenue, Madison, WI 53706, USA.
authors.forename: Joanne L
authors.initials: JL
authors.lastname: Ekena
authors.forename: Brynne C
authors.initials: BC
authors.lastname: Stanton
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Schiebe-Owens
authors.forename: Christina M
authors.initials: CM
authors.lastname: Hull
mesh_terms: D015152:Blotting, Northern; D015139:Blotting, Southern; D003455:Cryptococcus neoformans / Q000254:growth & development* / Q000472:pathogenicity; D004171:Diploidy; D024202:Electrophoretic Mobility Shift Assay; D048690:Fruiting Bodies, Fungal; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D049770:Genes, Mating Type, Fungal; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D011401:Promoter Regions, Genetic / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D018398:Homeodomain Proteins; D014157:Transcription Factors