title: [Nimesulide spectrophotometric determination in the visible region].
abstract: Nimesulide is a nonsteroidal antiinflammatory (NSAID) drug whose mechanism of action is characterized by selective inhibition of cyclooxygenase-2. Nimesulide have analgesic and antipyretic properties.
  
  
  METHODS
  For nimesulide analysis we developed a spectrophotometric method based on reduction of the nitro group of nimesulide by zinc and hydrochloric acid followed by diazotization and coupling with thymol (5-methyl-2-(1-methylethyl) phenol) in ammonia medium to form a stable chromophore which absorbs at 476 nm. There were established the practical working conditions. The developed method was validated.
  
  
  RESULTS
  The method showed a good linearity in the range of 5 - 40 microg / sample (the correlation coefficient r = 0.9990). The detection limit (LD) was 0.99 microg/sample and the quantification limit (LQ) was 3.32 microg/sample. There were established the system precision (RSD = 1.70%), the precision (RSD < 2.2%) and the accuracy (recovery in the range 97.66 - 102.69% with a mean recovery of 99.81%) of the method.
  
  
  CONCLUSIONS
  A new method for nimesulide spectrophotometric quantification in visible has been developed.
authors.affiliation: Facultatea de Farmacie, Disciplina de Chimie analitică, Universitatea de Medicină i Farmacie "Gr.T. Popa" Iaşi.
authors.forename: Andreia Claudia
authors.initials: AC
authors.lastname: Perju
authors.forename: Mariana
authors.initials: M
authors.lastname: Mândrescu
authors.forename: A F
authors.initials: AF
authors.lastname: Spac
authors.forename: V
authors.initials: V
authors.lastname: Dorneanu
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000493:pharmacokinetics; D016861:Cyclooxygenase Inhibitors / Q000032:analysis* / Q000493:pharmacokinetics; D006801:Humans; D013053:Spectrophotometry / Q000379:methods*; D013449:Sulfonamides / Q000032:analysis* / Q000493:pharmacokinetics
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016861:Cyclooxygenase Inhibitors; D013449:Sulfonamides; C012655:nimesulide