Detection of sequence-specific protein-DNA interactions via surface enhanced resonance Raman scattering.
We developed a new strategy to detect protein-DNA binding through surface enhanced resonance Raman scattering (SERRS). Silver-plated DNA and gold nanoparticle assemblies were used to identify sequence-specific and concentration-dependent binding of a DNA cytosine-C5-methyltransferase and the eukaryotic transcriptional regulator, TATA binding protein. Proteins were identified through specific Raman-active labels, and affinities observed correlate well with those determined by other methods. Raman-active labeling and interchangeable DNA sequences create a platform for versatile investigation of multiprotein complexes.
Bonham
Andrew J
AJ
Department of Chemistry and Biochemistry, The University of California Santa Barbara, Santa Barbara, California 93106-9510, USA.
Braun
Gary
G
Pavel
Ioana
I
Moskovits
Martin
M
Reich
Norbert O
NO
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
D011506:Proteins; D004247:DNA