title: Ginsenoside Rd production from the major ginsenoside Rb(1) by beta-glucosidase from Thermus caldophilus.
abstract: Under optimum conditions (pH 5, 75 degrees C, and 0.2 U purified enzyme ml(-1)), 4 mg ginsenoside Rd was produced from 5 mg reagent-grade ginsenoside Rb(1) in 5 ml after 30 min by beta-glucosidase from Thermus caldophilus GK24. Using a ginseng root extract containing 1 mg ginsenoside Rb(1) ml(-1) and 3.2 mg additional ginsenosides ml(-1), 1.23 mg ginsenoside Rd ml(-1) was produced after 18 h; the concentrations of ginsenosides Rb(1), Rb(2), and Rc used for ginsenoside Rd production were 0.77, 0.17, and 0.19 mg ml(-1), respectively.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, South Korea.
authors.forename: Ju-Wan
authors.initials: JW
authors.lastname: Son
authors.forename: Hye-Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D036145:Ginsenosides / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D005894:Panax / Q000737:chemistry; D010936:Plant Extracts / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D013696:Temperature; D013824:Thermus / Q000201:enzymology*; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D036145:Ginsenosides; D010936:Plant Extracts; D001617:beta-Glucosidase; C049863:ginsenoside Rd