title: Influence of vehicle on kinetics of exogenous progesterone administered either by subcutaneous and intramuscular routes to sheep.
abstract: This study aimed to determine the best vehicle and administration route for progesterone administration in sheep. In a first replicate, single intramuscular doses of 25mg progesterone were administered to ewes previously ovariectomized, either in propylene glycol (group IM-PG, n=6) or olive oil (group IM-OO, n=5). In a second replicate, the same solutions of progesterone were administered subcutaneously to the same ewes (groups SC-PG, n=6, and SC-OO, n=5). In the present study, the best pharmacokinetic results of a single dose of 25mg of progesterone were obtained, both using PG and OO as vehicles, by the subcutaneous route. Thus, progesterone remained in plasma for a longer time after subcutaneous administration in PG than in OO (t(1/2beta): 60.65+/-13.07 vs. 27.51+/-3.59 h; P<0.05); the mean residence time being higher in SC-PG than in SC-OO group (88.99+/-18.36 vs. 41.04+/-5.31h; P<0.05). However, both vehicles allowed maintained plasma levels 0.5 ng/ml for at least 30 h, so any of these treatments may be efficiently used for administration of exogenous progesterone.
authors.affiliation: Departamento de Reproducción Animal, INIA, Av. Puerta de Hierro, s/n, Madrid 28040, Spain. ignacio.contreras@ucv.ve
authors.forename: I
authors.initials: I
authors.lastname: Contreras-Solis
authors.forename: A
authors.initials: A
authors.lastname: Gomez-Brunet
authors.forename: T
authors.initials: T
authors.lastname: Encinas
authors.forename: A
authors.initials: A
authors.lastname: Gonzalez-Bulnes
authors.forename: J
authors.initials: J
authors.lastname: Santiago-Moreno
authors.forename: A
authors.initials: A
authors.lastname: Lopez-Sebastian
mesh_terms: D000818:Animals; D019540:Area Under Curve; D002626:Chemistry, Pharmaceutical; D005260:Female; D006207:Half-Life; D007273:Injections, Intramuscular; D007279:Injections, Subcutaneous; D014677:Pharmaceutical Vehicles / Q000008:administration & dosage / Q000493:pharmacokinetics; D011374:Progesterone / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D012756:Sheep / Q000378:metabolism*
chemical_list: D014677:Pharmaceutical Vehicles; D011374:Progesterone