title: Diplogonoporiasis in Japan: genetic analyses of five clinical isolates.
abstract: Infection of the whale tapeworm Diplogonoporus balaenopterae (Diphyllobothriidae) is occasionally found in humans, especially among Japanese. In the present study, we analysed the nucleotide sequences of the 18S rDNA, ITS1 and cox1 genes of the immature and mature proglottids of Diplogonoporus species recovered from five Japanese patients. The nucleotide sequences of 18S rDNA, ITS1 and cox1 showed little, if any, intraspecific divergence. Phylogenetic analyses of several diphyllobothriid species revealed a close relationship of Diplogonoporus isolates with the cetacean tapeworm Diphyllobothrium stemmacephalum. The results suggest that the genus Diphyllobothrium is paraphyletic and raise a question regarding the validity of the genus Diplogonoporus.
authors.affiliation: Department of Medical Zoology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. arizonon@koto.kpu-m.ac.jp
authors.forename: Naoki
authors.initials: N
authors.lastname: Arizono
authors.forename: Soji
authors.initials: S
authors.lastname: Fukumoto
authors.forename: Sayuri
authors.initials: S
authors.lastname: Tademoto
authors.forename: Minoru
authors.initials: M
authors.lastname: Yamada
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Uchikawa
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tegoshi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kuramochi
mesh_terms: D000818:Animals; D002589:Cestoda / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002590:Cestode Infections / Q000469:parasitology*; D051545:Cyclooxygenase 1 / Q000235:genetics; D018509:DNA, Helminth / Q000032:analysis / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics; D005260:Female; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S; D051545:Cyclooxygenase 1