Labedella gwakjiensis gen. nov., sp. nov., a novel actinomycete of the family Microbacteriaceae.
A Gram-positive, non-motile, non-mycelium-forming, rod-shaped actinomycete, designated KSW2-17T, was isolated from dried seaweed collected from beach sand along the coast of Jeju, Republic of Korea. The organism had ornithine as the diagnostic cell-wall diamino acid, MK-10 and MK-11 as the major menaquinones, and phosphatidylglycerol and diphosphatidylglycerol as polar lipids. The fatty acid profile included predominantly iso- and anteiso-branched acids and a minor amount of tuberculostearic acid (10-methyl C18:0). The DNA G+C content was 68.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the seaweed isolate formed a distinct clade within the radiation of the family Microbacteriaceae and had highest sequence similarity (96.1-96.3%) to members of the genera Cryobacterium, Frigoribacterium and Rathayibacter. On the basis of phenotypic and genotypic evidence, strain KSW2-17T is considered to represent a novel species of a new genus, for which the name Labedella gwakjiensis gen. nov., sp. nov. is proposed. The type strain is KSW2-17T (=JCM 14008T=KCTC 19176T).
Lee
Soon Dong
SD
Department of Science Education, Cheju National University, Jeju 690-756, Republic of Korea.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D007723:Korea; D008969:Molecular Sequence Data; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012624:Seaweed / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S