title: Aging-related oxidative stress in healthy humans.
abstract: Oxidative stress has been reported to increase with aging; however, the scientific evidence is controversial. We therefore aimed to analyze the relationship between aging and some markers of oxidative stress. A cross-sectional and comparative study was carried out in a sample of 249 healthy subjects: (i) 25-29 years (n = 22); (ii) 30-39 years (24); (iii) 40-49 years (30); (iv) 50-59 years (48); (v) 60-69 years (60), and (vi) >or= 70 years (65). We measured lipoperoxides and total antioxidant status in plasma and superoxide dismutase and glutathione peroxidase activities in erythrocytes. There was an age-related increase in lipoperoxides, which was evident in the comparison of the group of 25-29 years (0.22 0.11 micromol/l) with the group of 60-69 years (0.38 +/- 0.18 micromol/l, p < 0.01) and >or= 70 years (0.42 +/- 0.19, p < 0.001). Conversely, the total antioxidant status showed an age-related decrease (25-29 years, 1.4 +/- 0.31 mmol/l vs 60-69 years, 1.1 +/- 0.21 and >or= 70 years, 1.1 +/- 0.22, p < 0.05 for each). In erythrocytes, glutathione peroxidase activity showed an age-related decrease (25-29 years, 7,966 +/- 1,813 UI/l vs 60-69 years, 6,193 +/- 2,235 and >or= 70 years, 6,547 +/- 2,307, p < 0.001 for each), whereas superoxide dismutase activity was similar in all age groups. Importantly, there was no age-related change in oxidative stress markers in subjects of < 60 years. These findings suggest that age of >or= 60 years may be associated with increased oxidative stress.
authors.affiliation: Gerontology and Research Unit, FES-Zaragoza, UNAM, Mexico City, Mexico. mendovic@servidor.unam.mx
authors.forename: Víctor Manuel
authors.initials: VM
authors.lastname: Mendoza-Núñez
authors.forename: Mirna
authors.initials: M
authors.lastname: Ruiz-Ramos
authors.forename: Martha A
authors.initials: MA
authors.lastname: Sánchez-Rodríguez
authors.forename: Raquel
authors.initials: R
authors.lastname: Retana-Ugalde
authors.forename: José Luis
authors.initials: JL
authors.lastname: Muñoz-Sánchez
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000375:Aging; D000975:Antioxidants / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006454:Hemoglobins; D006801:Humans; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances
chemical_list: D000975:Antioxidants; D006454:Hemoglobins; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase