Anti-obesity and anti-tumor pro-apoptotic peptides are sufficient to cause release of cytochrome c from vesicles.
Peptides that target tissue for an apoptotic death have potential as therapeutics in a variety of disease conditions. The class of peptides described herein enters the cell through a specific receptor-mediated interaction. Once inside the cell, the peptide migrates toward the mitochondria, where the membrane barrier is disrupted. These experiments demonstrate that upon treatment with these short peptides large unilamellar vesicles are not lysed, a graded mode of leakage is observed and the transient pores formed by these peptides are large enough to release entrapped cytochrome c from the vesicles.
Sandoval
Cristina M
CM
Department of Chemistry and Biochemistry, University of San Diego, 5998 Alcala Park, San Diego, CA 92110, USA.
Salzameda
Bridget
B
Reyes
Kristine
K
Williams
Taylor
T
Hohman
Valerie S
VS
Plesniak
Leigh A
LA
D000595:Amino Acid Sequence; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D002850:Chromatography, Gel; D045304:Cytochromes c / Q000378:metabolism*; D008823:Micelles; D008969:Molecular Sequence Data; D009765:Obesity / Q000201:enzymology; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology
D000970:Antineoplastic Agents; D008823:Micelles; D010455:Peptides; D045304:Cytochromes c