title: Quantitation of B. Pseudomallei in clinical samples.
abstract: We undertook a prospective study to quantitate Burkholderia pseudomallei in blood, urine, respiratory secretions, and pus [corrected] obtained from 414 patients with melioidosis. The median was count 1.1, 1.5 x 10(4), 1.1 x 10(5), and 1.1 x 10(7) CFU/mL in these sample types, respectively. This provides important insights into the likely feasibility of future studies such as expression microarray analysis using clinical material.
authors.affiliation: Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Vanaporn
authors.initials: V
authors.lastname: Wuthiekanun
authors.forename: Direk
authors.initials: D
authors.lastname: Limmathurotsakul
authors.forename: Gumphol
authors.initials: G
authors.lastname: Wongsuvan
authors.forename: Wirongrong
authors.initials: W
authors.lastname: Chierakul
authors.forename: Nittaya
authors.initials: N
authors.lastname: Teerawattanasook
authors.forename: Prapit
authors.initials: P
authors.lastname: Teparrukkul
authors.forename: Nicholas P
authors.initials: NP
authors.lastname: Day
authors.forename: Sharon J
authors.initials: SJ
authors.lastname: Peacock
mesh_terms: D000293:Adolescent; D000328:Adult; D001431:Bacteriological Techniques; D016957:Burkholderia pseudomallei / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D008554:Melioidosis / Q000097:blood / Q000382:microbiology* / Q000652:urine; D013183:Sputum / Q000382:microbiology; D013492:Suppuration / Q000382:microbiology