title: Prognostic value of LISCH7 mRNA in plasma and tumor of colon cancer patients.
abstract: OBJECTIVE
  LISCH7 is a gene potentially regulated by p53 that is up-regulated in metastasis development. Our hypothesis was that the expression of LISCH7 in primary colorectal tumors determined certain characteristics of the tumors, as well as their behavior, and that its identification in plasma could serve as a prognostic marker.
  
  
  METHODS
  We tested this hypothesis in a series of 115 tumors and normal tissues and in 83 plasmas from patients with sporadic colorectal carcinomas, as well as in 20 healthy control plasmas in which the expression levels of the gene were measured by real-time PCR. The expression data were contrasted with clinicopathologic variables.
  
  
  RESULTS
  Although LISCH7 expression was not detected in any control plasma samples, it was positive in 25 (30.1%) plasmas from patients (P = 0.002). LISCH7 mRNA in plasma was significantly associated with the pathologic stage (P = 0.019), with lymph node metastasis (P = 0.008) and with vascular invasion (P = 0.005). Expression was not detected in any normal tissues but was detected in 80 tumor tissues, with a clear association found with vascular invasion (P = 0.027). Moreover, we show that LISCH7 was specifically expressed by the epithelial tumor cells. The adjusted overall survival study showed independent prognostic values for LISCH7 expression levels in tumor tissues (hazard ratio, 3.45; 95% confidence interval, 1.19-9.98).
  
  
  CONCLUSIONS
  Our results suggest that LISCH7 is a good tumor marker whose expression levels could be considered as a poor prognosis factor in human colon cancer. Furthermore, plasma is suggested as a feasible source of nucleic acids for subsequent noninvasive prognostic studies.
authors.affiliation: Department of Medical Oncology, Hospital Universitario Puerta de Hierro, Madrid, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: García
authors.forename: Cristina
authors.initials: C
authors.lastname: Peña
authors.forename: Vanesa
authors.initials: V
authors.lastname: García
authors.forename: Gemma
authors.initials: G
authors.lastname: Domínguez
authors.forename: Concepción
authors.initials: C
authors.lastname: Muñoz
authors.forename: Javier
authors.initials: J
authors.lastname: Silva
authors.forename: Isabel
authors.initials: I
authors.lastname: Millán
authors.forename: Raquel
authors.initials: R
authors.lastname: Diaz
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Lorenzo
authors.forename: Rufo
authors.initials: R
authors.lastname: Rodriguez
authors.forename: Félix
authors.initials: F
authors.lastname: Bonilla
mesh_terms: D000368:Aged; D003110:Colonic Neoplasms / Q000097:blood* / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism*; D011973:Receptors, LDL / Q000097:blood* / Q000378:metabolism*; D018110:Receptors, Lipoprotein / Q000096:biosynthesis* / Q000235:genetics*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics*; D016896:Treatment Outcome; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: C525854:LSR protein, human; D012333:RNA, Messenger; D011973:Receptors, LDL; D018110:Receptors, Lipoprotein; C495901:TP53 protein, human; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; C085992:lipolysis-stimulated receptor