Detection of HHV-8 and HPV in laryngeal carcinoma.
OBJECTIVE
In this study we aimed to investigate the presence of human herpesvirus 8 (HHV-8) and human papillomavirus (HPV) in laryngeal carcinoma.


METHODS
Fifty patients operated on because of laryngeal carcinoma were included in the study. Forty-seven had squamous cell carcinoma (SCC) whereas three had verrucous carcinoma. Fresh tumoral tissues, or tumoral tissues obtained from archival paraffin-embedded blocks, were examined. HHV-8 DNA and HPV DNA were detected using polymerase chain reaction (PCR) and viral genotypes of HPV were determined via the hybrid capture method. The presence of HHV-8 DNA and HPV DNA were also investigated in normal appearing laryngeal tissue collected from 50 cadavers at autopsy.


RESULTS
HPV DNA was detected in seven patients (7/50; 14%) (5 out of 47 patients with SCC (5/47; 10.6%) and two out of three patients with verrucous carcinoma). HHV-8 DNA was detected in five patients and they all had SCC (5/47; 10.6%). One case had both HHV-8 and HPV DNA. None of the control samples from cadavers harbored HHV-8 DNA, or HPV DNA. There was a statistically significant correlation between HHV-8 DNA and HPV DNA positivity and laryngeal SCC (Fisher exact test; p=0.023 for each). No statistically significant correlations were found between the presence of HHV-8 and/or HPV and age, gender, tumor stage, differentiation, the site of the tumor, smoking and alcohol use.


CONCLUSIONS
The findings of the present study suggest that beside HPV, HHV-8 might have a role in laryngeal carcinogenesis. Further investigations are necessary to clarify the exact role of these viruses in laryngeal carcinoma.
Güvenç
Melih Güven
MG
Istanbul University, Cerrahpaşa Medical Faculty, Department of Otorhinolaryngology, Turkey. guvencmg@yahoo.com
Midilli
Kenan
K
Ozdoğan
Ahmet
A
Inci
Ender
E
Tahamiler
Rauf
R
Enver
Ozgün
O
Sirin
Gözde
G
Ergin
Sevgi
S
Kuşkucu
Mert
M
Divanoğlu
Emel Ozergil
EO
Yilmaz
Gülden
G
Altas
Kemal
K
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D018289:Carcinoma, Verrucous / Q000473:pathology / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005838:Genotype; D019288:Herpesvirus 8, Human / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology / Q000821:virology*; D007830:Larynx / Q000473:pathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction
D004279:DNA, Viral