Tracking antigen specific CD4+ T-cells with soluble MHC molecules.
The advent of soluble MHC multimer technology has allowed for the flow-cytometric direct identification of specific-MHC restricted antigen-specific T cells in mixed cell populations and also enabled the direct phenotyping and cloning of these cells at the same time. To date, MHC multimers have been used in characterizing the adaptive T cell repertoire under infectious, cancerous, and autoimmune states and has increased our understanding of the dynamics of T-cell immunity. Recombinant MHC multimers have been produced where MHC-binding peptide antigens are either covalently or noncovalently bound to the MHC, with the latter having the advantage of the ability to use a single recombinant MHC to investigate multiple MHC-binding peptides and their interacting T cells. In this method we describe how to generate recombinant non-covalently bound peptide MHC-multimers in insect cells. MHC multimers are generated as tetravalent complexes using a streptavidin scaffold.
Gebe
John A
JA
Benaroya Research Institute, Seattle, WA, USA.
Kwok
William W
WW
D000818:Animals; D000941:Antigens / Q000276:immunology*; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D007313:Insecta; D008285:Major Histocompatibility Complex; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D010455:Peptides; D011994:Recombinant Proteins