title: Characterisation of complex amphiphilic cyclodextrin mixtures by high-performance liquid chromatography and mass spectrometry.
abstract: It is established that amphiphilic beta-cyclodextrins chemically modified with alkyl chains on the secondary face exhibit self-organisation properties yielding stable nanospheres or nanoparticles. The ability of these promising colloidal drug carriers to encapsulate drugs being partly related to the internal structure of nanosystems, precise characterisation methods are required to control their synthesis procedure. The present work describes the development of complementary analytical methods based on reversed-phase high-performance liquid chromatography (RPLC) coupled to evaporative light-scattering detection (ELSD) and electrospray ionisation-mass spectrometry (ESI-MS) to characterize various beta-cyclodextrins enzymatically transesterified by vinyl-acyl fatty esters (the number of carbon atom in the acyl chain varying from 4 to 12). LC-ELSD has been used in a preliminary step to optimize the separation on a monolithic octadecylsiloxane-bonded silica stationary phase. A complex fingerprint was achieved for each mixture, revealing the presence of isomers unnoticed by the sole spectrometric (NMR and MS) techniques.
authors.affiliation: Institut de Chimie Organique et Analytique (ICOA), Université d'Orléans, UFR Sciences, UMR CNRS 6005, BP 6759, rue de Chartres, F-45067 Orléans cedex 2, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Kieken
authors.forename: Caroline
authors.initials: C
authors.lastname: West
authors.forename: Karim
authors.initials: K
authors.lastname: Keddadouche
authors.forename: Claire
authors.initials: C
authors.lastname: Elfakir
authors.forename: Luc
authors.initials: L
authors.lastname: Choisnard
authors.forename: A
authors.initials: A
authors.lastname: Gèze
authors.forename: Denis
authors.initials: D
authors.lastname: Wouessidjewe
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003505:Cyclodextrins / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D003505:Cyclodextrins