title: Identification and isolation of synovial dendritic cells.
abstract: In rheumatoid arthritis patients, three compartments need to be considered: peripheral blood, synovial fluid, and synovial tissue. Dendritic cells characterized from each compartment have different properties. The methods given are based on cell sorting for isolation of cells, and flow cytometry and immunohistochemical staining for analysis of cells in these compartments.
authors.affiliation: Centre for Immunology and Cancer Research, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
authors.forename: Allison R
authors.initials: AR
authors.lastname: Pettit
authors.forename: Lois
authors.initials: L
authors.lastname: Cavanagh
authors.forename: Amanda
authors.initials: A
authors.lastname: Boyce
authors.forename: Jagadish
authors.initials: J
authors.lastname: Padmanabha
authors.forename: Judy
authors.initials: J
authors.lastname: Peng
authors.forename: Ranjeny
authors.initials: R
authors.lastname: Thomas
mesh_terms: D000818:Animals; D039521:CD11c Antigen / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D003713:Dendritic Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D053650:Interleukin-3 Receptor alpha Subunit / Q000378:metabolism; D016185:Receptors, Interleukin-3 / Q000378:metabolism; D013582:Synovial Fluid / Q000166:cytology* / Q000276:immunology
chemical_list: D039521:CD11c Antigen; C115022:IL3RA protein, human; D053650:Interleukin-3 Receptor alpha Subunit; D016185:Receptors, Interleukin-3