[Isolation and characterization of lectin from the surface of Grifola frondosa (FR.) S.F. Gray mycelium].
For the first time, from the surface of the dikaryotic mycelium of the xylotrophic basidiomycete Grifola frondosa 0917 a lectin has been isolated with a molecular mass of 68 +/- 1 kDa, consisting of two subunits of 33-34 kDa each. The lectin is a hydrophilic glycoprotein with the protein : glycan ratio of 3 : 1. It exhibits high affinity to native rabbit erythrocytes and to human erythrocytes of the 0 blood group, but not to trypsin-treated ones. The hemagglutination (HA) caused by lectin was not blocked by any of the 25 tested mono-, di-, and amino sugars; it was also not blocked by some of glyco derivatives. Only 13.9 microg/ml of the homogeneous preparation of a polysaccharide, a linear D-rhamnan with the structure of the repetitive component --> 2)-beta-D-Rhap-(1 --> 3)-alpha-D-Rhap-(1 --> 3)-alpha-D-Rhap-(1 --> 2)-alpha-D-Rhap-(1 --> 2)-alpha-D-Rhap-(1 --> blocked hemagglutination completely. The analysis of the amino acid composition of the lectin showed the greatest percentage of amino acids with positively charged R groups, arginine, lysine, and histidine, as well as the complete absence of sulfur-containing amino acids, cysteine, and methionine. D-glucose and D-glucosamine were detected in the carbohydrate part.
Stepanova
L V
LV
Nikitina
V E
VE
Boĭko
A S
AS
D000596:Amino Acids / Q000032:analysis; D000606:Amino Sugars / Q000494:pharmacology; D000818:Animals; D002240:Carbohydrate Sequence; D003837:Deoxy Sugars / Q000494:pharmacology; D004187:Disaccharides / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects*; D048691:Grifola / Q000737:chemistry*; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008351:Mannans / Q000494:pharmacology; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000494:pharmacology; D011817:Rabbits
D000596:Amino Acids; D000606:Amino Sugars; D003837:Deoxy Sugars; D004187:Disaccharides; D037102:Lectins; D008351:Mannans; D009005:Monosaccharides; C065836:rhamnan