title: A high resolution structure of the putative hinge region in M2 channel-lining segments of the nicotinic acetylcholine receptor.
abstract: Transmembrane peptide helices play key roles in signal transduction across cell membranes, yet little is known about their high-resolution structure or the role membrane composition plays in their association, structure, dynamics and ultimately their performance. Using magic angle spinning (MAS) homonuclear dipolar recoupling experiments, the backbone structure at positions L10, L11, and A12 of the M2 ion channel peptide was determined in two lipid systems. Their measurements are in agreement with M2 forming transmembrane helices, but the torsion angles vary considerably from common alpha-helical values. These measurements show remarkable agreement with a previous computational model of M2 peptides forming a pore domain in which their helices are kinked near the central leucine, L11 [R. Sankararamakrishnan, C. Adcock, M.S.P. Sansom, The pore domain of the nicotinic acetylcholine receptor: Molecular modeling, pore dimensions, and electrostatics, Biophys. J. 71 (1996) 1659-1671]. The generation of high resolution data for transmembrane helices is of critical importance in refining structures for membrane protein and developing models of helix packing interactions.
authors.affiliation: Department of Biochemistry and Molecular Biology and McKnight Brain Institute, University of Florida, Gainesville, FL 32611, USA. jrlong@mbi.ufl.edu
authors.forename: Joanna R
authors.initials: JR
authors.lastname: Long
authors.forename: Frank D
authors.initials: FD
authors.lastname: Mills
authors.forename: Frank
authors.initials: F
authors.lastname: Raucci
mesh_terms: D003198:Computer Simulation; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011978:Receptors, Nicotinic / Q000737:chemistry*
chemical_list: D010455:Peptides; D011978:Receptors, Nicotinic