Detection of antibodies to egg drop syndrome virus in chicken serum using a field-based immunofiltration (flow-through) test.
A simple, user-friendly, and rapid method to detect the presence of antibodies to egg drop syndrome 76 (EDS) virus in chicken sera based on an immunofiltration (flow-through) test was developed. Purified EDS virus antigen was coated onto nitrocellulose membranes housed in a plastic module with layers of absorbent filter pads underneath. Following addition of serum to be tested and washing, monoclonal antibodies or polyclonal serum to chicken immunoglobulin G (IgG) was used as a bridge antibody to mediate binding between EDS virus-specific IgG and protein A gold conjugate. The appearance of a pink dot indicated the presence of antibodies to EDS virus in the sample tested. The results could be obtained within 5-10 min. The developed immunofiltration test could detect antibodies in the sera of experimentally vaccinated chickens from 2 wk postvaccination. With field sera samples, this test was positive in samples having hemagglutination inhibition titers of 8 and above. This test has the potential to be used as a field-based kit to assess seroconversion in EDS-vaccinated flocks.
Raj
G Dhinakar
GD
Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India. dhinakarraj@yahoo.com
Thiagarajan
V
V
Nachimuthu
K
K
D000257:Adenoviridae Infections / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D046808:Atadenovirus / Q000276:immunology*; D002645:Chickens / Q000097:blood / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D007118:Immunoassay / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases / Q000097:blood / Q000276:immunology* / Q000821:virology; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral