Cell microarray for functional exploration of genomes.
As more genomes are sequenced, challenge of rapidly unraveling the functions of genes was faced. To that end, cell microarrays have been recently described that permit transfection of thousands of nucleic acids in parallel and enable the analysis of phenotypic consequences of such perturbations. As many parameters can influence the efficiency of transfection and consequently protein expression or extinction, some important features in manufacturing cell microarrays for functional exploration of genomes were described.
Castel
David
D
CEA, DSV, DRR, Functional Genomics Department, Evry, France.
Debily
Marie-Anne
MA
Pitaval
Amandine
A
Gidrol
Xavier
X
D000818:Animals; D002478:Cells, Cultured; D020868:Gene Silencing; D016678:Genome; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D009696:Nucleic Acids / Q000378:metabolism; D046888:Tissue Array Analysis / Q000379:methods*; D014162:Transfection
D009696:Nucleic Acids; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins