Direct monitoring pressure overload predicts cardiac hypertrophy in mice.
Pressure overload (POL) is a classical model for studying cardiac hypertrophy, but there has been no direct measure of hemodynamics in a conscious ambulatory mouse model of POL. We used abdominal aortic constriction to produce POL and radiotelemetry to measure the blood pressure and heart rate for three weeks. The cardiac size correlated with the systolic pressure in the last week is better than other hemodynamic parameters. Cardiac fibrosis was more correlated to the cardiac size than to the systolic pressure. The expression of the cardiac genes that are typically associated with cardiac hypertrophy was correlated with both cardiac size and systolic pressure. In conclusion, the systolic pressure is the major determinant of cardiac hypertrophy in the murine POL model. In contrast, cardiac fibrosis shows the influence of other factors besides systolic pressure. The combination of the POL model with continuous direct measurements of hemodynamics represents a significant technological advance and will lead to an extended usefulness of POL methodologically.
Duan
Sheng Zhong
SZ
Department of Molecular and Integrative Physiology, University of Michigan Medical School, 7744 Med. Sci. II, 1150 W. Med. Ctr. Dr., Ann Arbor, MI 48109-0622, USA.
Ivashchenko
Christine Y
CY
Whitesall
Steven E
SE
D'Alecy
Louis G
LG
Mortensen
Richard M
RM
D000818:Animals; D001794:Blood Pressure; D001795:Blood Pressure Determination / Q000295:instrumentation / Q000379:methods; D006332:Cardiomegaly / Q000209:etiology* / Q000235:genetics / Q000503:physiopathology; D005355:Fibrosis / Q000209:etiology / Q000235:genetics / Q000503:physiopathology; D005786:Gene Expression Regulation / Q000502:physiology; D006321:Heart / Q000503:physiopathology; D006339:Heart Rate / Q000502:physiology; D006973:Hypertension / Q000150:complications* / Q000175:diagnosis* / Q000503:physiopathology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D023421:Models, Animal; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology; D011237:Predictive Value of Tests; D011846:Radio Waves; D013599:Systole / Q000502:physiology; D013686:Telemetry / Q000379:methods