Amino acid production from a sunflower wholemeal protein concentrate.
A study was undertaken to investigate the influence of protein concentration and the addition of different doses of endopeptidase (Alcalase) and exopeptidase (Flavourzyme) on the sequential enzymatic hydrolysis of a protein concentrate obtained from defatted sunflower wholemeal. The results show that the greatest degree of hydrolysis (37.8%) is achieved by hydrolyzing an aqueous substrate with a 5% protein concentrate, and using a 0.02 g Alcalase/g of protein concentrate of the substrate. The aminograms performed reveal that the free amino acid found in the highest proportion in the hydrolysate was aspartic acid, which accounted for over 50% of the free amino acids present, regardless of the substrate concentration and the enzyme dosage used. Finally, the hydrolysate obtained from a substrate containing a 5% protein concentrate and a 0.02 g Alcalase/g of protein concentrate displayed characteristics that indicate its suitability for use as a vegetable-origin plant growth regulator.
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Departamento de Química Agrícola y Edafología, Facultad de Ciencias, Universidad de Córdoba, Campus de Rabanales, Edificio Marie Curie, Ctra. Nacional IV-a, km. 336, 14014 Córdoba, Spain.
Benítez
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González
J L
JL
D000596:Amino Acids / Q000096:biosynthesis*; D002850:Chromatography, Gel; D006368:Helianthus / Q000378:metabolism*; D006868:Hydrolysis; D010940:Plant Proteins / Q000378:metabolism*; D013381:Subtilisins / Q000378:metabolism
D000596:Amino Acids; D010940:Plant Proteins; D013381:Subtilisins