Rotation algorithm for determining cycle thresholds in Real-Time Polymerase Chain Reactions.
The Real Time-Polymerase Chain Reaction (RT-PCR) is a methodology that is widely used to amplify and quantify specific sections of nucleic acid strands. A key aspect of RT-PCR is to determine the reaction cycle that produces a positive PCR signal. This cycle is proportional to the relative concentration of nucleic acid and produces a linear curve when plotted vs. the log of the sample concentration. The cycle that produces a positive PCR signal is called the elbow or more precisely the Cycle Threshold (Ct) value. This paper describes a novel Rotation Algorithm that determines the Ct value and is less susceptible to signal interferences.
Kurnik
Ronald T
RT
Roche Molecular Systems, Pleasanton, CA 94588, USA. ronald.kurnik@roche.com
D000465:Algorithms; D019295:Computational Biology / Q000295:instrumentation / Q000379:methods*; D008433:Mathematics; D015233:Models, Statistical; D008962:Models, Theoretical; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods; D012815:Signal Processing, Computer-Assisted; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013997:Time Factors