Production and characterization of a monoclonal antibody against GPR40 (FFAR1; free fatty acid receptor 1).
GPR40 is G protein-coupled receptor whose endogenous ligands have recently been identified as free fatty acids (FFAs), and it has been implicated to play an important role in FFA-mediated enhancement of glucose-stimulated insulin release. We have developed a monoclonal antibody against the extracellular domain of GPR40. Specificity of the antibody was demonstrated by immunoprecipitation and cell surface staining using GPR40-transfected cells. GPR40 immunoreactivity was highly abundant in mouse pancreatic beta-cells and splenocytes, THP-1 cells, and human peripheral blood mononuclear cells. The anti-GPR40 monoclonal antibody should prove valuable for further studying the function of this nutrient sensing receptor.
Hirasawa
Akira
A
Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-shimo-adachi-cho, Sakyo-ku, Kyoto 606-8501, Japan. akira_h@pharm.kyoto-u.ac.jp
Itsubo
Chisato
C
Sadakane
Keiko
K
Hara
Takafumi
T
Shinagawa
Shingo
S
Koga
Hanako
H
Nose
Hiroshi
H
Koshimizu
Taka-aki
TA
Tsujimoto
Gozoh
G
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology*; D000918:Antibody Specificity; D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000032:analysis / Q000276:immunology*; D014162:Transfection
D000911:Antibodies, Monoclonal; C473034:FFAR1 protein, human; C473036:Ffar1 protein, mouse; D010446:Peptide Fragments; D043562:Receptors, G-Protein-Coupled