title: Effect of prolonged ketamine exposure on cardiovascular physiology in pregnant and infant rhesus monkeys (Macaca mulatta).
abstract: Physiologic measurements in nonhuman primates usually are collected from animals that are chemically or physically restrained. Both types of restraint may affect the parameters measured, and those effects can vary with age. Heart rate, respiratory rate, oxygen saturation, expired CO2, blood pressure, temperature, blood glucose, hematocrit, and venous blood gasses were measured in rhesus monkeys that were either infused intravenously with ketamine for 24 h or were cage-housed and physically restrained for sample collection. The subjects were pregnant monkeys at gestational day 120 to 123, infants 5 to 6 d old, and infants 35 to 37 d old. Heart rate and blood pressure were lower in ketamine-treated monkeys than physically restrained monkeys. Heart rate was higher in infants than adults, whereas blood pressure was lower in infants. Respiratory rate was higher in infants than adults and higher in physically restrained infants than ketamine-sedated infants but was not affected by ketamine in pregnant adults. Hematocrit was decreased in older infants. In summary, both physical restraint and ketamine sedation altered several physiologic parameters in pregnant and infant rhesus macaques. Investigators should consider these effects when designing experiments and evaluating experimental outcomes in monkeys.
authors.affiliation: The Bionetics Corporation, National Center for Toxicological Research /FDA, Jefferson, AR, USA. chotchki@wanprc.org
authors.forename: Charlotte E
authors.initials: CE
authors.lastname: Hotchkiss
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.forename: William
authors.initials: W
authors.lastname: Slikker
mesh_terms: D000778:Anesthetics, Dissociative / Q000494:pharmacology*; D000818:Animals; D000830:Animals, Laboratory; D000831:Animals, Newborn / Q000502:physiology*; D001784:Blood Gas Analysis; D001786:Blood Glucose / Q000187:drug effects; D001794:Blood Pressure / Q000187:drug effects; D002320:Cardiovascular Physiological Phenomena / Q000187:drug effects*; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D006400:Hematocrit; D006799:Housing, Animal; D007649:Ketamine / Q000494:pharmacology*; D008253:Macaca mulatta / Q000502:physiology*; D008297:Male; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D015203:Reproducibility of Results; D015656:Respiratory Mechanics / Q000187:drug effects; D012149:Restraint, Physical / Q000502:physiology / Q000662:veterinary; D013997:Time Factors
chemical_list: D000778:Anesthetics, Dissociative; D001786:Blood Glucose; D007649:Ketamine