[Study of HPLC-DAD fingerprint on complex traditional Chinese medicine proprietary preparation-Baoji pills].
OBJECTIVE
Based on 'Back-tracking' method, identification and quality evaluation of complex traditional Chinese medicine (TCM) preparation of Baoji pills (BJP) were carried out by HPLC fingerprint analysis.


METHODS
HPLC-DAD fingerprint of BJP was conducted with Zorbax SB-C18 column and non-linear elution with the mobile phase consisted of acetonitrile-0.5% glacial acetic acid at column temperature 30 degrees C and detective wavelengths of 250 nm and 283 nm. From the established chromatographic pattern of BJP, track backward to the corresponding crude herbal drugs in the formula, attribution ofmost peaks in the BJP fingerprint can be disclosed.


RESULTS
The BJP HPLC fingerprint consisted of 44 peaks among which 35 peaks were assigned by parallel comparison with the fingerprint of the 10 corresponding crude drugs in the formula such as pueraria, pummelo peel, and magnolia bark, etc. and 22 peaks we reidentified by comparison with the chemical reference substances.


CONCLUSIONS
The established HPLC fingerprint represents the whole character of BJP, which enhanced the specialty for control and assessment of the product quality. It exemplified much more effective for quality control than selecting any marker for qualitative or quantitative testing target. And the Back-tracking' experimental method extended the study mentality for complex formula TCM products chromatographic fingerprinting analysis.
Wang
Xiang-hong
XH
Zhuhai Chromap Institute of Herbal Medicine Research, Zhuhai 519085, China.
Xie
Pei-shan
PS
Tian
Run-tao
RT
Huang
Xiao-dan
XD
Zheng
Rong-bo
RB
Qin
Chun-mei
CM
Yu
Qiong-xi
QX
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031202:Chrysanthemum / Q000737:chemistry; D002957:Citrus / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D031566:Magnolia / Q000737:chemistry; D008516:Medicine, Chinese Traditional; D010946:Plants, Medicinal / Q000737:chemistry*; D029908:Pueraria / Q000737:chemistry; D015203:Reproducibility of Results
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C532976:baoji