title: Sample preparation.
abstract: A panorama of sample preparation methods has been composed from 481 references, with a highlight of some promising methods fast developed during recent years and a somewhat brief introduction on most of the well-developed methods. All the samples were commonly referred to molecular composition, being extendable to particles including cells but not to organs, tissues and larger bodies. Some criteria to evaluate or validate a sample preparation method were proposed for reference. Strategy for integration of several methods to prepare complicated protein samples for proteomic studies was illustrated and discussed.
authors.affiliation: Beijing National Laboratory of Molecular Science, Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China. chenyi@iccas.ac.cn
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhenpeng
authors.initials: Z
authors.lastname: Guo
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.forename: Changgui
authors.initials: C
authors.lastname: Qiu
mesh_terms: D000327:Adsorption; D005591:Chemical Fractionation / Q000379:methods*; D002849:Chromatography, Gas / Q000379:methods; D025924:Chromatography, Supercritical Fluid / Q000379:methods; D004586:Electrophoresis / Q000379:methods; D005374:Filtration; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids; D007525:Isoelectric Focusing / Q000379:methods; D017551:Microdialysis; D008904:Miniaturization; D011312:Pressure; D052617:Solid Phase Microextraction / Q000379:methods; D013048:Specimen Handling / Q000379:methods*; D054928:Validation Studies as Topic
chemical_list: D052578:Ionic Liquids