[Effects of kaixinsan on behavior and expression of p-CREB in hippocampus of chronic stress rats].
OBJECTIVE
To study the effects of kaixinsan on behavior and expression of p-CREB in hippocampus of the chronic stress rats.


METHODS
The male Wistar rats which gained the similar scores by Open-field test were selected, then the depression model rat was produced by separation and chronic unpredictable mild stress. Open-field test was performed to detect the behavior of rats and immunohistochemistry was used to observe the changes of p-CREB expression in hippocampus.


RESULTS
On the 22nd day, the body weight and sacchar-intake of the depression model rats were all lower than those of the normal rats and the body weight and sacchar-intake of the rats treated by drugs were higher compared with model rats (P < 0.01). The depressive behavior in kaixinsan 4 g x kg(-1) group was significantly improved compared with the model group, the crossing scores and rearing scores were all increased (P < 0.01) and the expression of p-CREB in CA1, CA3 and DG in hippocampus was higher than that in the model group (P < 0.05 and P < 0.01).


CONCLUSIONS
Kaixinsan might improve depressive behavior by increasing expression of p-CREB in CA1, CA3 and DG in hippocampus of the chronic stress rats.
Wang
Jin-liang
JL
Dept. of Clinical Pharmacology, General Hospital of PLA, Beijing 100853, China.
Liu
Ping
P
Tu
Hai-hua
HH
Wang
Zhong-xiao
ZX
Chen
Gui-yun
GY
D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology; D001522:Behavior, Animal / Q000187:drug effects*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D003863:Depression / Q000209:etiology / Q000503:physiopathology; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000150:complications
D000928:Antidepressive Agents; D017362:Cyclic AMP Response Element-Binding Protein; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal