title: Single lymphocyte analysis with a microwell array chip.
abstract: Following genomics and proteomics, cytomics, a novel method of looking at life, has emerged for analyzing large populations of cells on a single-cell basis with multiple parameters in a quantitative manner. We have developed a highly integrated live-cell microarray system for analyzing the cellular responses of individual cells using a microwell array chip that has 234,000 microwells each of which is just large enough to fit a single cell. Compared with flow cytometry and microscope-based methods, our system can analyze the history of the cellular responses of a large number of cells. We have successfully applied the system to analyze human antigen-specific B-cells and produced human monoclonal antibodies (MoAb) against hepatitis B virus surface antigen. We have also constructed a mouse system to assess hepatitis B virus-neutralization activity and have demonstrated the neutralization activity of our antibodies. Our technology should expand the horizons of cell analysis as well as enable generation of human MoAb for antibody-based therapeutics and diagnosis for infectious diseases such as hepatitis viruses.
authors.affiliation: Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Tokimitsu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kishi
authors.forename: Sachiko
authors.initials: S
authors.lastname: Kondo
authors.forename: Ritsu
authors.initials: R
authors.lastname: Honda
authors.forename: Kazuto
authors.initials: K
authors.lastname: Tajiri
authors.forename: Kazumi
authors.initials: K
authors.lastname: Motoki
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Ozawa
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kadowaki
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Obata
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fujiki
authors.forename: Chise
authors.initials: C
authors.lastname: Tateno
authors.forename: Hideki
authors.initials: H
authors.lastname: Takaishi
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Chayama
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Yoshizato
authors.forename: Eiichi
authors.initials: E
authors.lastname: Tamiya
authors.forename: Toshiro
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Muraguchi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods; D006509:Hepatitis B / Q000276:immunology / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000097:blood / Q000276:immunology; D017325:Hepatitis B Vaccines / Q000097:blood / Q000276:immunology; D019694:Hepatitis B, Chronic / Q000276:immunology; D022781:Hepatocytes / Q000276:immunology / Q000821:virology; D006801:Humans; D008213:Lymphocyte Activation; D051379:Mice; D016513:Mice, SCID; D008822:Mice, Transgenic; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines