Trichinella spiralis induces de novo expression of group IVC phospholipase A2 in the intestinal epithelium.
Phospholipase A2 (PLA2) enzymes play a central role in the initiation, propagation and resolution of inflammation. Here, we describe de novo expression of group IVC PLA2 (PLA2g4c) within the intestinal epithelium of Trichinella spiralis parasitised mice. This mouse mast cell protease-1 sensitive, calcium-independent PLA2 is not detectable in the jejunal epithelium of uninfected mice but becomes highly expressed within the epithelial compartment within days of nematode establishment. We propose that epithelial PLA2g4c accounts for the increased lysophospholipase activity observed during intestinal nematodiasis and that it plays a major role in the inflammatory response to nematodes.
Brown
Jeremy K
JK
Division of Veterinary Clinical Studies, The University of Edinburgh, Easter Bush Veterinary Centre, Midlothian, EH25 9RG, United Kingdom.
Knight
Pamela A
PA
Thornton
Elisabeth M
EM
Pate
Judith A
JA
Coonrod
Scott
S
Miller
Hugh R P
HR
Pemberton
Alan D
AD
D000818:Animals; D053818:Chymases / Q000378:metabolism; D015870:Gene Expression; D054513:Group IV Phospholipases A2 / Q000235:genetics* / Q000378:metabolism; D007249:Inflammation; D007411:Intestinal Diseases, Parasitic / Q000201:enzymology*; D007413:Intestinal Mucosa / Q000201:enzymology*; D007583:Jejunum; D051379:Mice; D008807:Mice, Inbred BALB C; D017160:Trichinella spiralis / Q000502:physiology*; D014235:Trichinellosis / Q000201:enzymology*
D054513:Group IV Phospholipases A2; D053818:Chymases; C509230:Mcpt1 protein, mouse