Microarray analysis of bisphenol A-induced changes in gene expression in human oral epithelial cells.
Bisphenol A (BPA) is a common ingredient in dental materials. However, its potential adverse effects on the oral cavity are unknown. The purpose of this study is to identify the genes responding to BPA in a human oral epithelial cell line using DNA microarray. Of the 10,368 genes examined, changes in mRNA levels were detected in seven genes: five were up-regulated and two were down-regulated. The expression levels of the calcium channel, voltage-dependent, L-type, alpha 1C subunit (CACNA1C), cell death activator CIDE-3 (CIDE-3), haptoglobin-related protein (HPR), importin 4 (IPO4), and POU domain, class 2 and transcription factor 3 (POU2F3) were significantly up-regulated in the cells exposed to 100 mM BPA. The spermatogenesis-associated, serine-rich 2 (SPATS2) and HSPC049 protein (HSPC049) were significantly down-regulated. The detailed knowledge of the changes in gene expression obtained using microarray technology will provide a basis for further elucidating the molecular mechanisms of the toxic effects of BPA in the oral cavity.
Seki
Keisuke
K
General Practice Residency, Nihon University School of Dentistry Dental Hospital, Tokyo 101-8310, Japan.
Koshi
Ryosuke
R
Sugano
Naoyuki
N
Masutani
Shigeyuki
S
Yoshinuma
Naoto
N
Ito
Koichi
K
D001559:Benzhydryl Compounds; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects* / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D009061:Mouth Mucosa / Q000187:drug effects / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010636:Phenols / Q000008:administration & dosage*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D006360:Heat-Shock Proteins; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds