Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy.
As an alternative approach to conventional allergen-specific immunotherapy, transgenic rice seed expressing a major house dust mite (HDM) allergen, Der p 1, was developed as an edible vaccine. The C-terminal KDEL-tagged Der p 1 allergen specifically accumulated in seed endosperm tissue under the control of the endosperm-specific GluB1 promoter. Der p 1 reached a maximum concentration of 58 microg/grain and was deposited in the endoplasmic reticulum (ER)-derived protein body I (PB-I). Plant-derived Der p 1 was posttranslationally modified with high-mannose-type glycan structures. Glycosylated Der p 1 displayed reduced IgE binding capacity in comparison with its unglycosylated counterpart in vitro. Our results indicate that transgenic Der p 1 rice seeds are a safe, potential oral delivery vaccine for the treatment of HDM allergy.
Yang
Lijun
L
Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan.
Kajiura
Hiroyuki
H
Suzuki
Kazuya
K
Hirose
Sakiko
S
Fujiyama
Kazuhito
K
Takaiwa
Fumio
F
D000818:Animals; D039741:Antigens, Dermatophagoides / Q000235:genetics / Q000276:immunology* / Q000378:metabolism* / Q000627:therapeutic use; D060829:Arthropod Proteins; D003546:Cysteine Endopeptidases; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods; D039981:Pyroglyphidae / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism*; D022462:Vaccines, Edible / Q000276:immunology* / Q000378:metabolism* / Q000627:therapeutic use
D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D011994:Recombinant Proteins; D022462:Vaccines, Edible; D003546:Cysteine Endopeptidases; C451162:Dermatophagoides pteronyssinus antigen p 1