title: Chlorogenic acid reduces the plasma glucose peak in the oral glucose tolerance test: effects on hepatic glucose release and glycaemia.
abstract: The effects of chlorogenic acid (CA) on hepatic glucose output, blood glucose levels and on glucose tolerance were analysed. Hepatic uptake of CA and its effects on hepatic catabolism of L-alanine and glucose-6-phosphatase (G-6-Pase) activity were also evaluated. CA (1 mM) inhibited about 40% of G-6-Pase activity (p < 0.05) in the microsomal fraction of hepatocytes, but no effect was observed on production of glucose from gluconeogenesis or on L-alanine catabolism, at various concentrations of CA (0.33, 0.5 and 1 mM), in liver perfusion experiments. Since there were indications of a lack of uptake of CA by the liver, it is possible that this compound did not reach sufficiently high intracellular levels to inhibit the target enzyme. Accordingly, intravenous administration of CA also failed to provoke a reduction in blood glucose levels. However, CA did promote a significant reduction (p < 0.05) in the plasma glucose peak at 10 and 15 min during the oral glucose tolerance test, probably by attenuating intestinal glucose absorption, suggesting a possible role for it as a glycaemic index lowering agent and highlighting it as a compound of interest for reducing the risk of developing type 2 diabetes.
authors.affiliation: Department of Physiological Sciences, State University of Londrina, Londrina, PR, Brazil.
authors.forename: Bruna Kempfer
authors.initials: BK
authors.lastname: Bassoli
authors.forename: Priscila
authors.initials: P
authors.lastname: Cassolla
authors.forename: Glaucia Regina
authors.initials: GR
authors.lastname: Borba-Murad
authors.forename: Jorgete
authors.initials: J
authors.lastname: Constantin
authors.forename: Clairce Luzia
authors.initials: CL
authors.lastname: Salgueiro-Pagadigorria
authors.forename: Roberto Barbosa
authors.initials: RB
authors.lastname: Bazotte
authors.forename: Rui Sérgio dos Santos Ferreira
authors.initials: RS
authors.lastname: da Silva
authors.forename: Helenir Medri
authors.initials: HM
authors.lastname: de Souza
mesh_terms: D000818:Animals; D019540:Area Under Curve; D001786:Blood Glucose / Q000187:drug effects*; D002726:Chlorogenic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D005947:Glucose / Q000096:biosynthesis; D005951:Glucose Tolerance Test; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D019344:Lactic Acid / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D019289:Pyruvic Acid / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014508:Urea / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D002726:Chlorogenic Acid; D019344:Lactic Acid; D019289:Pyruvic Acid; D014508:Urea; D005952:Glucose-6-Phosphatase; D005947:Glucose; D005990:Glycerol