Novel mass tags for single nucleotide polymorphism detection.
A new method suitable for single nucleotide polymorphism detection and other applications based on oligonucleotide probe extension has been developed. The method is based on mass spectrometry and utilizes a single surface for affinity purification of extended probes and matrix-independent desorption/ionization of the cleavable labels. A new family of sulfur-linked laser-cleavable trityl labels with vastly improved flying abilities is implemented in this study. Corresponding reagents compatible with automated oligonucleotide synthesis are presented. Utility of this method for SNP genotyping is demonstrated.
Birikh
Klara R
KR
Shemyakin-Ovchinikov Institute of Bioorganic Chemistry, RAS, Miklukho-Maklaya 16/10, 117997, Moscow, Russia.
Korshun
Vladimir A
VA
Bernad
Pablo L
PL
Malakhov
Andrei D
AD
Milner
Natalie
N
Khan
Safraz
S
Southern
Edwin M
EM
Shchepinov
Mikhail S
MS
D004252:DNA Mutational Analysis / Q000379:methods*; D017931:DNA Primers / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D005838:Genotype; D006046:Gold / Q000737:chemistry; D013058:Mass Spectrometry; D008957:Models, Genetic; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009696:Nucleic Acids / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013499:Surface Properties
D017931:DNA Primers; D009696:Nucleic Acids; D006046:Gold