title: Coupling X-ray and optical tomography systems for in vivo examination of small animals.
abstract: For the purpose of the co-registration of fluorescence optical signal and X-rays measurements, a multimodality tomographer has been developed in our laboratory. Such a system brings the possibility to get, on the same bench, in vivo both anatomical and functional information. Moreover, the information on the morphology of the animal can be used as a regularization factor in order to get the reconstructions of the biodistribution of fluorochromes more accurate and to reduce the computation time. A study on homogeneous phantoms was conducted to evaluate the feasibility, to test the linearity and the reproducibility, and to fix the parameters for the co-registration. More cumbersome phantoms (sacrificed mice) have then been considered and the test experiments were reproduced. Finally, results of a study conducted in vivo on mice bearing tumors in the lungs, tagged with different types of optical probes, are presented.
authors.affiliation: CEA-LETI MINATEC, 17 rue des Martyrs F-38054 Grenoble Cedex 9, France. anabela.dasilva@cea.fr
authors.forename: Anabela
authors.initials: A
authors.lastname: da Silva
authors.forename: Thomas
authors.initials: T
authors.lastname: Bordy
authors.forename: Mathieu
authors.initials: M
authors.lastname: Debourdeau
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Dinten
authors.forename: Philippe
authors.initials: P
authors.lastname: Peltié
authors.forename: Philippe
authors.initials: P
authors.lastname: Rizo
mesh_terms: D000818:Animals; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041622:Tomography, Optical / Q000295:instrumentation* / Q000662:veterinary*; D014057:Tomography, X-Ray Computed / Q000295:instrumentation* / Q000662:veterinary*; D051598:Whole Body Imaging / Q000295:instrumentation* / Q000662:veterinary*