title: Nisin-triggered activity of Lys44, the secreted endolysin from Oenococcus oeni phage fOg44.
abstract: The intrinsic resistance of Oenococcus oeni cells to the secreted endolysin from oenophage fOg44 (Lys44) was investigated. Experiments with several antimicrobials support the hypothesis that the full activity of Lys44 requires sudden ion-nonspecific dissipation of the proton motive force, an event undertaken by the fOg44 holin in the phage infection context.
authors.affiliation: Instituto de Ciência Aplicada e Tecnologia da Faculdade de Ciências da Universidade de Lisboa, 1749-016 Lisboa, Portugal.
authors.forename: João Gil
authors.initials: JG
authors.lastname: Nascimento
authors.forename: Maria Carolina
authors.initials: MC
authors.lastname: Guerreiro-Pereira
authors.forename: Sérgio Fernandes
authors.initials: SF
authors.lastname: Costa
authors.forename: Carlos
authors.initials: C
authors.lastname: São-José
authors.forename: Mário Almeida
authors.initials: MA
authors.lastname: Santos
mesh_terms: D001435:Bacteriophages / Q000472:pathogenicity / Q000502:physiology*; D002473:Cell Wall / Q000502:physiology / Q000821:virology; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D007113:Immunity, Innate; D007777:Lactobacillaceae / Q000502:physiology / Q000821:virology*; D009561:Nisin / Q000378:metabolism*; D014764:Viral Proteins / Q000502:physiology*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D009561:Nisin; D004247:DNA