Effect of the 1BL.1RS translocation on the wheat endosperm, as revealed by proteomic analysis.
The introduction of the 1RS chromosome of rye into wheat made wheat more resistant to several pathogens. Today, this resistance has been overcome but the 1BL.1RS translocation remains interesting because of the improved yield and despite the lower rheological properties it produces. Nothing has been reported yet on the impact of rye chromatin introgression on the grain proteome of wheat. The comparison of the 2-DE profiles of 16 doubled haploid lines, with or without the 1BL.1RS translocation, revealed quantitative and qualitative proteic variations in prolamins and other endosperm proteins. Eight spots were found specifically in lines having the 1BL.1RS translocation; 16 other spots disappeared from the same lines. Twelve spots, present in both genotypes, met the criteria for up- or down-regulated spots. In translocated genotypes, a highly overexpressed spot, identified as a gamma-gliadin with nine cysteine residues, suggests that the lack of LMW-GS induced by 1BL.1RS is counterbalanced by an overexpression of a relatively similar prolamin. Moreover, a spot that was absent from 1BL.1RS genotypes was identified as a dimeric alpha-amylase inhibitor. It was considered to be a valuable candidate to explain the sticky dough associated with translocated cultivars.
Gobaa
Samy
S
Swiss Federal Institute of Technology Zurich (ETHZ), Institute of Plant Sciences, Universitätsrasse 2, Switzerland. samy.gobaa@ipw.agrl.ethz.ch
Bancel
Emmanuelle
E
Kleijer
Geert
G
Stamp
Peter
P
Branlard
Gérard
G
D032461:Chromosomes, Plant / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D024861:Genetic Enhancement; D005903:Gliadin / Q000032:analysis; D005983:Glutens / Q000032:analysis; D008970:Molecular Weight; D010940:Plant Proteins / Q000032:analysis*; D030821:Plants, Genetically Modified; D055315:Prolamins; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D012434:Secale / Q000235:genetics; D012639:Seeds / Q000737:chemistry / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014178:Translocation, Genetic; D014908:Triticum / Q000737:chemistry* / Q000235:genetics
D010940:Plant Proteins; D055315:Prolamins; D020543:Proteome; C060586:WDAI-3 protein, Triticum aestivum; D005983:Glutens; D005903:Gliadin; C037302:glutenin