Heat-resistant DNA tile arrays constructed by template-directed photoligation through 5-carboxyvinyl-2'-deoxyuridine.
Template-directed DNA photoligation has been applied to a method to construct heat-resistant two-dimensional (2D) DNA arrays that can work as scaffolds in bottom-up assembly of functional biomolecules and nano-electronic components. DNA double-crossover AB-staggered (DXAB) tiles were covalently connected by enzyme-free template-directed photoligation, which enables a specific ligation reaction in an extremely tight space and under buffer conditions where no enzymes work efficiently. DNA nanostructures created by self-assembly of the DXAB tiles before and after photoligation have been visualized by high-resolution, tapping mode atomic force microscopy in buffer. The improvement of the heat tolerance of 2D DNA arrays was confirmed by heating and visualizing the DNA nanostructures. The heat-resistant DNA arrays may expand the potential of DNA as functional materials in biotechnology and nanotechnology.
Tagawa
Miho
M
Department of Life Sciences and Institute of Physics, Graduate School of Arts and Sciences, The University of Tokyo, Japan.
Shohda
Koh-ichiroh
K
Fujimoto
Kenzo
K
Sugawara
Tadashi
T
Suyama
Akira
A
D020029:Base Pairing; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects* / Q000648:ultrastructure; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry; D006358:Hot Temperature; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology; D010777:Photochemistry; D014466:Ultraviolet Rays
C520201:5-carboxyvinyl-2'-deoxyuridine; D004247:DNA; D003857:Deoxyuridine