title: Effects of dietary lactosucrose (4G-beta-D-galactosylsucrose) on the IgE response in mice.
abstract: In this study, we examined the effects of dietary lactosucrose (LS, a non-digestible oligosaccharide) on the IgE response in mice immunized with ovalbumin (OVA)/alum. In addition to IgG1 and IgG2a responses, the anti-OVA IgE response in mice fed LS diets was dose-dependently suppressed, as compared with the control mice, while the serum total IgG levels were comparable. Moreover, dietary LS feeding inhibited antigen-specific IgE and IgG1 productions even after a second immunization. Regarding with cytokine production, when stimulated in vitro with OVA, splenocytes obtained from LS-fed mice produced a similar level of IFN-gamma, and lower levels of IL-4 and IL-5, as compared with the control mice. But IL-10 production by OVA-stimulated splenocytes was augmented in LS-fed mice, suggesting that IL-10 producing cells are responsible for the immunoregulatory effect of LS. Our findings indicate the further possibility that dietary LS supplementation can be used to prevent IgE-mediated allergic diseases.
authors.affiliation: Biomedical Institute, Research Center, Hayashibara Biochemical Laboratories, Inc. Okayama 702-8006, Japan. ytani@hayashibara.co.jp
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Taniguchi
authors.forename: Akiko
authors.initials: A
authors.lastname: Mizote
authors.forename: Keizo
authors.initials: K
authors.lastname: Kohno
authors.forename: Kanso
authors.initials: K
authors.lastname: Iwaki
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Oku
authors.forename: Hiroto
authors.initials: H
authors.lastname: Chaen
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Fukuda
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000485:Allergens / Q000276:immunology; D000534:Alum Compounds / Q000008:administration & dosage; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D006967:Hypersensitivity / Q000276:immunology / Q000517:prevention & control*; D007114:Immunization; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000097:blood; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007165:Immunosuppression Therapy; D051379:Mice; D010047:Ovalbumin / Q000276:immunology; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D014312:Trisaccharides / Q000008:administration & dosage*
chemical_list: D000276:Adjuvants, Immunologic; D000485:Allergens; D000534:Alum Compounds; D016207:Cytokines; D004040:Dietary Carbohydrates; D007074:Immunoglobulin G; D014312:Trisaccharides; C041524:aluminum sulfate; D007073:Immunoglobulin E; C081752:galactosucrose; D010047:Ovalbumin