Blastocyst-derived trophoblast stem cells from the rhesus monkey.
Although trophoblast stem cells can be obtained directly from blastocyst outgrowths in the mouse, this has never been described in primates. In human and non-human primates, trophoblast cells have been obtained from embryonic stem (ES) cells or embryoid bodies (EBs). The results reported here show for the first time that cells with the characteristics of trophoblast stem cells can be derived directly from rhesus monkey blastocyst outgrowths. The cells expressed trophoblast markers and were maintained for multiple passages in the absence of feeder layers or growth factors. The cells could be maintained as adherent, mononuclear cells by regular passaging, but they formed syncytial-like structures if maintained in culture for prolonged periods or if incubated in the presence of 17beta-estradiol. The cells also demonstrated invasive behavior similar to extravillous trophoblasts. The availability of these lines provides a useful experimental system for studying trophoblast differentiation and for developing novel intervention strategies to treat placental dysfunction.
Vandevoort
Catherine A
CA
California National Primate Research Center, Department of Obstetrics and Gynecology, School of Medicine, University of California, Davis CA 95616, USA.
Thirkill
Twanda L
TL
Douglas
Gordon C
GC
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology; D005786:Gene Expression Regulation; D015726:Giant Cells / Q000166:cytology; D007150:Immunohistochemistry; D008253:Macaca mulatta / Q000196:embryology*; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism
D015415:Biomarkers; D050814:Octamer Transcription Factor-3