title: Putative bioactive conformations of amide linked cyclic myelin basic protein peptide analogues associated with experimental autoimmune encephalomyelitis.
abstract: The solution models of cyclo(87-99) MBP87-99, cyclo(87-99) [Ala91,96] MBP87-99, and cyclo(87-99) [Arg91, Ala96] MBP87-99 have been determined through 2D NMR spectroscopy in DMSO-d6. Chemical shift analysis has been performed in an attempt to elucidate structural changes occurring upon substitution of native residues. NMR-derived geometrical constraints have been used in order to calculate high-resolution conformers of the above peptides. Conformational analysis of the three synthetic analogues show that the bioactivity, or the lack of it, may possibly be due to the distinct local structure observed and the subsequent differences in the overall topology and exposed area after binding with Major Histocompatibility Complex II (MHC II). It is believed that an overall larger solvent accessible area blocks the approach and binding of the T-cell receptor (TCR) on the altered peptide ligand (APL)-MHC complex, whereas more compact structures do not occlude weak interactions with an approaching TCR and can cause Experimental Autoimmune Encephalomyelitis (EAE) antagonism. A pharmacophore model based on the structural data has been generated.
authors.affiliation: Departments of Pharmacy and Chemistry, University of Patras, Patras, Greece.
authors.forename: Zinovia
authors.initials: Z
authors.lastname: Spyranti
authors.forename: Georgios A
authors.initials: GA
authors.lastname: Dalkas
authors.forename: Georgios A
authors.initials: GA
authors.lastname: Spyroulias
authors.forename: Efthimia D
authors.initials: ED
authors.lastname: Mantzourani
authors.forename: Thomas
authors.initials: T
authors.lastname: Mavromoustakos
authors.forename: Irene
authors.initials: I
authors.lastname: Friligou
authors.forename: John M
authors.initials: JM
authors.lastname: Matsoukas
authors.forename: Theodore V
authors.initials: TV
authors.lastname: Tselios
mesh_terms: D003500:Cyclization; D003903:Deuterium; D004121:Dimethyl Sulfoxide; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D004676:Myelin Basic Protein / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry*; D011487:Protein Conformation; D012996:Solutions
chemical_list: D004676:Myelin Basic Protein; D010456:Peptides, Cyclic; D012996:Solutions; D003903:Deuterium; D004121:Dimethyl Sulfoxide