In vitro activity of kaempferol isolated from the Impatiens balsamina alone and in combination with erythromycin or clindamycin against Propionibacterium acnes.
The in vitro antibacterial activity against antibiotic-resistant Propionibacterium acnes of kaempferol isolated from the Impatiens balsamina alone and in combination with erythromycin or clindamycin antibiotics was investigated. The antibiotic combination effect against antibiotic-resistant P. acnes was studied by checkerboard test. Kaempferol and quercetin demonstrated antibacterial activities against P. acnes. Minimum inhibitory concentrations (MICs) for both compounds were < or =32 ug/ml and < or =64 ug/ml for clindamycin-sensitive and-resistant P. acnes, respectively. The four combination formulations (kaempferol and either erythromycin or clindamycin; quercetin and either erythromycin or clindamycin) exhibited a synergic inhibition of P. acnes growth. The combination of kaempferol with quercetin showed an indifferent effect. The combination of clindamycin with kaempferol or quercetin showed a greater synergic effect than that of erythromycin with kaempferol or quercetin. Thus, these combinations demonstrated the potential to treat acne.
Lim
Young-Hee
YH
Department of Clinical Laboratory Science, College of Health Sciences, Korea University, Seoul, Republic of Korea. yhlim@korea.ac.kr
Kim
In-Hwan
IH
Seo
Jung-Ju
JJ
D000667:Ampicillin / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D002981:Clindamycin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D004357:Drug Synergism; D004917:Erythromycin / Q000494:pharmacology*; D029069:Impatiens / Q000737:chemistry*; D044949:Kaempferols / Q000302:isolation & purification* / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011425:Propionibacterium acnes / Q000187:drug effects* / Q000254:growth & development; D013752:Tetracycline / Q000494:pharmacology
D044949:Kaempferols; D002981:Clindamycin; D004917:Erythromycin; D002701:Chloramphenicol; C006552:kaempferol; D000667:Ampicillin; D013752:Tetracycline