title: Chronic alcohol abuse and endosseous implants: linkage of in vitro osteoblast dysfunction to titanium osseointegration rate.
abstract: Chronic alcohol consumption is associated with pathological effects on bone, and it is correlated with the increasing risk of osteoporosis and fractures. The negative effects of alcohol intake also influence bone repair processes and the osseointegration of implants. The aim of the present in vitro study was to investigate the proliferation and synthetic activity of osteoblasts isolated from the trabecular bone of rats previously exposed to 7-week intermittent exposure to ethanol vapour (EE-OB), and sham-aged rats (SA-OB), when cultured on standard commercially pure Ti (cpTi). Osteoblast proliferation (WST-1), alkaline phosphatase (ALP), osteocalcin (OC), collagen type I (CICP), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and transforming growth factor-beta1 (TGF-beta1) were measured at 1, 7, and 14 days of culture. Our results showed a decrease in the cell viability and synthetic activity of osteoblasts exposed to ethanol when cultured on cpTi. Moreover, the release of local regulatory factors from osteoblasts was imbalanced: TGF-beta1 production was reduced and TNF-alpha and IL-6 were up-regulated. These in vitro data suggest that alcohol abuse affects bone repair and decreases the ability to form bone around standard cpTi. Innovative surfaces and adjuvant therapies could be useful when implants are required in alcoholics.
authors.affiliation: Laboratory of Experimental Surgery, Research Institute Codivilla-Putti, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136 Bologna, Italy. paola.torricelli@ior.it
authors.forename: Paola
authors.initials: P
authors.lastname: Torricelli
authors.forename: Milena
authors.initials: M
authors.lastname: Fini
authors.forename: Gianluca
authors.initials: G
authors.lastname: Giavaresi
authors.forename: Lia
authors.initials: L
authors.lastname: Rimondini
authors.forename: Matilde
authors.initials: M
authors.lastname: Tschon
authors.forename: Roberto
authors.initials: R
authors.lastname: Rimondini
authors.forename: Antonio
authors.initials: A
authors.lastname: Carrassi
authors.forename: Roberto
authors.initials: R
authors.lastname: Giardino
mesh_terms: D000437:Alcoholism / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D000431:Ethanol / Q000633:toxicity; D008297:Male; D016348:Osseointegration / Q000187:drug effects*; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014835:Volatilization
chemical_list: D000431:Ethanol; D014025:Titanium