title: Blunted metabolic response to fasting in obese mice.
abstract: The aim of the study was to evaluate metabolic changes in response to fasting in normal and obese mice. C57BL6 and obese (diet-induced obesity (DIO) and ob/ob) mice were used in this study. They were fasted for 24 h and re-fed for 24 h. Body weight was monitored before, after fasting and during re-feeding (2 and 24 h after re-feeding). Food intake was measured 2 and 24 h after re-feeding began. Blood samples were taken before and after 24 h fasting. As metabolic parameters, blood glucose, plasma insulin, ghrelin levels and oxygen consumption were measured. Blood glucose and plasma insulin levels in DIO and ob/ob mice were higher than normal mice, and plasma ghrelin levels were lower in DIO and ob/ob mice. There was reduced body weight loss in DIO mice than in normal mice for 24 h fasting. When they were re-fed, DIO and ob/ob mice consumed less food intake than normal mice. Twenty-four hours food deprivation induced significantly smaller plasma ghrelin elevation in these obese mice. Fasting-induced decrease in oxygen consumption was significantly smaller in DIO and ob/ob mice than normal mice. This data show that obese mice may have decreased sensitivity to fasting-induced increase in circulating ghrelin and their oxygen consumption exhibited a blunted response to fasting.
authors.affiliation: Department of Internal Medicine, Kobe Seaside Hospital, 1-1-28 Isobe-dori Chuo-ku, Kobe, Hyogo, 651-0084, Japan. uenouf3@yahoo.co.jp
authors.forename: Naohiko
authors.initials: N
authors.lastname: Ueno
authors.forename: Akihiro
authors.initials: A
authors.lastname: Asakawa
authors.forename: Akio
authors.initials: A
authors.lastname: Inui
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000502:physiology; D004435:Eating / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology; D005215:Fasting / Q000378:metabolism*; D054439:Ghrelin / Q000097:blood*; D007328:Insulin / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology
chemical_list: D001786:Blood Glucose; D054439:Ghrelin; D007328:Insulin