title: Production and secretion of a recombinant Vibrio parahaemolyticus chitinase by Escherichia coli and its purification from the culture medium.
abstract: An open reading frame encoding the chitinase gene and its signal sequence was cloned from the Vibrio parahaemolyticus KN1699 genome. An expression plasmid containing the gene was introduced into Escherichia coli cells, and recombinant chitinase (Pa-rChi) was produced and secreted into the culture medium with the aid of the signal peptide. Pa-rChi was purified and its substrate specificity was determined.
authors.affiliation: Department of Biological Chemistry, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-8510, Japan.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Kadokura
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: Kaori
authors.initials: K
authors.lastname: Saito
authors.forename: Takanori
authors.initials: T
authors.lastname: Ikegami
authors.forename: Takako
authors.initials: T
authors.lastname: Hirano
authors.forename: Wataru
authors.initials: W
authors.lastname: Hakamata
authors.forename: Tadatake
authors.initials: T
authors.lastname: Oku
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Nishio
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D002688:Chitinases / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D003001:Cloning, Molecular; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D016680:Genome, Bacterial; D016366:Open Reading Frames / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification* / Q000378:metabolism; D014170:Transformation, Genetic; D014736:Vibrio parahaemolyticus / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D002688:Chitinases