Desulfatiferula olefinivorans gen. nov., sp. nov., a long-chain n-alkene-degrading, sulfate-reducing bacterium.
A novel anaerobic, long-chain alkene-degrading, sulfate-reducing bacterium, strain LM2801T, was isolated from brackish sediment of a wastewater decantation facility of an oil refinery (Berre lagoon, France). Cells of strain LM2801T were Gram-negative, motile, slightly curved or vibrioid rods. Its optimum growth conditions were 30-36 degrees C, 6-10 g NaCl l(-1) and pH 7.5. Strain LM2801T incompletely oxidized long-chain alkenes (from C14 to C23) and fatty acids (C14 to C24). The DNA G+C content was 45.5 mol%. Sequence analyses of the 16S rRNA and dsrAB genes indicated that the strain was a member of the family Desulfobacteraceae within the Deltaproteobacteria. This novel isolate possesses phenotypic and phylogenetic traits that do not allow its classification as a member of any previously described genus. Therefore, strain LM2801T is described as a member of a new genus, Desulfatiferula gen. nov., of which Desulfatiferula olefinivorans sp. nov. is the type species. The type strain of Desulfatiferula olefinivorans is LM2801T (=DSM 18843T=JCM 14469T).
Cravo-Laureau
Cristiana
C
Equipe Environnement et Microbiologie, IPREM UMR 5254, IBEAS Université de Pau et des Pays de l'Adour, BP1155, 64013 Pau cedex, France.
Labat
Cindy
C
Laboratoire de Microbiologie et Biotechnologie des Environnements Chauds, IRD, UMR_D 180 IFR-BAIM, case 925, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, 13288 Marseille cedex 9, France.
Joulian
Catherine
C
Laboratoire de Microbiologie et Biotechnologie des Environnements Chauds, IRD, UMR_D 180 IFR-BAIM, case 925, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, 13288 Marseille cedex 9, France.
Matheron
Robert
R
Laboratoire d'Ecologie Microbienne, IMEP UMR 6116, Université Paul Cézanne, Faculté des Sciences et Techniques de Saint-Jérôme, 13397 Marseille cedex 20, France.
Hirschler-Réa
Agnès
A
Laboratoire de Microbiologie et Biotechnologie des Environnements Chauds, IRD, UMR_D 180 IFR-BAIM, case 925, Universités de Provence et de la Méditerranée, 163 avenue de Luminy, 13288 Marseille cedex 9, France.
D000475:Alkenes / Q000737:chemistry / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020564:Deltaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D010578:Petroleum; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D016999:Sulfur-Reducing Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D014865:Waste Disposal, Fluid / Q000379:methods*; D014871:Water Microbiology
D000475:Alkenes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010578:Petroleum; D012336:RNA, Ribosomal, 16S