The essential fatty acid myristate causes severe growth retardation in Hpelo disruptants of the yeast Hansenula polymorpha.
Myristate (C14:0) is an essential multi-functional fatty acid in a variety of organisms. We found that C14:0, but not other fatty acids, causes severe growth retardation in Hpelo1Delta and Hpelo2Delta mutants of the yeast Hansenula polymorpha, defective in elongation of very long-chain fatty acids. While transcription of HpELO1 and HpELO2 is transiently increased by C14:0, this was not found to be responsible for the growth retardation. Transcription of HpFAS1 and HpFAS2 encoding fatty acid synthase is repressed by C14:0, but this repression was also not found to be responsible for growth retardation. A screen for suppressors that resulted in restored growth of the Hpelo1Delta disruptant on media containing C14:0 identified two types of suppressors. One exhibited a defect in C14:0 uptake while the other did not. Molecular genetic and genomic analysis of these suppressor mutations is anticipated to shed new light on the processes of fatty acid transport and the crucial role of C14:0 in the growth of eukaryotic organisms.
Prasitchoke
Phatthanon
P
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Kaneko
Yoshinobu
Y
Bamba
Takeshi
T
Fukusaki
Ei-ichiro
E
Kobayashi
Akio
A
Harashima
Satoshi
S
D053898:Biosynthetic Pathways; D003470:Culture Media / Q000737:chemistry; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D019814:Myristic Acid / Q000378:metabolism*; D010843:Pichia / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D013489:Suppression, Genetic
D003470:Culture Media; D005227:Fatty Acids; D005656:Fungal Proteins; D019814:Myristic Acid; D064429:Fatty Acid Synthases