title: Donor-specific antibodies by flow single antigen beads in pediatric living donor kidney transplants: single center experience.
abstract: Flow PRA and SAB are now routinely performed to identify HLA antibodies in the recipient sera. The presence of DSA is considered a risk factor for graft failure. However, this risk is not clearly defined. We reviewed all the pediatric recipients of living donor kidney transplants since Flow PRA and Flow SAB became routinely done at our institution. All children had negative CDC and Flow XMs. Comparison of clinical outcome was done between those with and without pretransplant DSA. Six children had positive DSA by Flow SAB. They received thymoglobulin, steroids, tacrolimus, and MMF. Five had anti-HLA class I antibodies and one anti-HLA class II. Only one child had an AR. Graft survival: 100% at last visit with GFR >70 mL/min/1.73 m(2). A control group without DSA was used for comparison (n = 44). They received our standard protocol: basiliximab, tacrolimus, MMF, and steroids. AR rate was 9%. Both groups had similar follow-up time, and patient and graft survival rates. In our small series, we saw excellent outcome despite the presence of DSA pretransplant. No unequivocal ideal to establish the ideal immunosuppressive regimen for this cohort of patients has been established and the long-term outcome of these recipients has not been delineated.
authors.affiliation: Pediatric Nephrology and Kidney Transplantation, Saint Barnabas Medical Center, Livingston, NJ 07039, USA. iroberti@sbhcs.com
authors.forename: Isabel
authors.initials: I
authors.lastname: Roberti
authors.forename: Shefali
authors.initials: S
authors.lastname: Vyas
authors.forename: Carol
authors.initials: C
authors.lastname: Pancoska
mesh_terms: D000293:Adolescent; D000906:Antibodies / Q000097:blood*; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry; D005500:Follow-Up Studies; D006084:Graft Rejection / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007223:Infant; D016030:Kidney Transplantation / Q000276:immunology*; D019520:Living Donors; D008297:Male; D008863:Microspheres; D011379:Prognosis; D012189:Retrospective Studies; D014184:Transplantation, Homologous
chemical_list: D000906:Antibodies; D006680:HLA Antigens