Wireless voltammetry recording in unanesthetised behaving rats.
In vivo voltammetry is a valuable technique for rapid measurement of dopamine in the brain of freely behaving rats. Using a conventional voltammetry system, however, behavioural freedom is restricted by cables connecting the head assembly to the measurement system. To overcome these difficulties, we developed a wireless voltammetry system utilizing radio waves. This system consisted of a potentiostat and transmitter system that was mounted on the back of the rat, and a receiver and analysis system. A single-step pulse (100-250 mV) was applied at 4 Hz after an activation pulse to a carbon fibre recording electrode (diameter: 7 microm). Measurement of dopamine (detection limit: 2.7 x 10(-7)M) was demonstrated in vitro. In vivo experiment was performed at least 1 week after the recording electrode was implanted in the rat striatum. Administration of 2-phenylethylamine to rats increased dopamine signal current, which was consistent with the result in the microdialysis measurement. During a resident-intruder test, dopamine signal current in a resident rat increased upon introduction of an intruder rat. These results show that the present wireless system is useful for a long-term measurement of dopamine in behaving rats.
Kagohashi
Maki
M
Department of Physiology, Juntendo University School of Medicine, Hongo 2-1-1, Tokyo 113-0033, Japan.
Nakazato
Taizo
T
Yoshimi
Kenji
K
Moizumi
Shunjiro
S
Hattori
Nobutaka
N
Kitazawa
Shigeru
S
D000758:Anesthesia / Q000379:methods; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D001923:Brain Chemistry / Q000187:drug effects / Q000502:physiology*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000032:analysis* / Q000378:metabolism; D004558:Electric Stimulation; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004567:Electrodes, Implanted / Q000592:standards; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D045604:Extracellular Fluid / Q000737:chemistry / Q000378:metabolism; D008297:Male; D017551:Microdialysis / Q000295:instrumentation / Q000379:methods; D009448:Neurochemistry / Q000295:instrumentation / Q000379:methods*; D010627:Phenethylamines / Q000494:pharmacology; D011619:Psychotropic Drugs / Q000494:pharmacology; D051381:Rats; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D012919:Social Behavior; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D013686:Telemetry / Q000295:instrumentation*; D013997:Time Factors; D014851:Wakefulness / Q000502:physiology
D010627:Phenethylamines; D011619:Psychotropic Drugs; C029261:phenethylamine; D004298:Dopamine