Identification of novel viral interleukin-10 isoforms of human cytomegalovirus AD169.
Two products of human cytomegalovirus (HCMV) UL111a gene have been previously identified to resemble human IL-10 (hIL-10). These viral IL-10s (vIL-10s) are able to induce signal transduction events and biological activities in a variety of cells. In this study, five novel vIL-10 transcripts were identified from HCMV AD169 infected MRC-5 cells. Some vIL-10 isoforms were post-translationally glycosylated, depending on the existence of a predicted N-linked glycosylation site. Similar to hIL-10, four of the vIL-10 isoforms apparently formed putative dimers. Among the different vIL-10 isoforms, vIL-10A significantly induced the phosphorylation of transcription factor STAT3 in THP-1 cells. All identified vIL-10 isoforms were able to form complexes with hIL-10, and enhanced hIL-10-induced STAT3 phosphorylation in different degrees. Identification of diverse forms of vIL-10 suggests that HCMV has developed a sophisticated mechanism to interfere with hIL-10 signaling pathway.
Lin
Yi-Ling
YL
Department of Oral Pathology, University of Kentucky College of Dentistry, 800 Rose Street, Lexington, KY 40536, USA.
Chang
Pei-Ching
PC
Wang
Yixiang
Y
Li
Mengtao
M
D000595:Amino Acid Sequence; D015153:Blotting, Western; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics / Q000502:physiology*; D019281:Dimerization; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011485:Protein Binding; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D050796:STAT3 Transcription Factor / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology
C446339:CMV IL-10 protein, Cytomegalovirus; D020033:Protein Isoforms; D012333:RNA, Messenger; D012367:RNA, Viral; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D014764:Viral Proteins