Effect of pH on the antimicrobial activity and oxidative stability of oil-in-water emulsions containing caffeic acid.
Antioxidant properties in food are dependent on various parameters. These include the pH value and interactions with food components, including proteins or metal ions. Food components affect antioxidant stability and also influence the properties of microorganisms and their viability. This paper describes an investigation of the effect of pH on the antioxidant and antibacterial properties of caffeic acid in different media. The pH values studied, using an oil-in-water emulsion as model system, were 3, 5 (with and without phosphate buffer), and 9. Effects of mixtures of caffeic acid, bovine serum albumin (BSA), and Fe (III) on oxidative deterioration in the emulsion samples were studied. The results show that the antioxidant activity of caffeic acid was increased by the presence of BSA. This effect was pH dependent and was affected by the presence of iron ions. Antibacterial properties were also pH dependent. The minimum concentration of caffeic acid required to inhibit some microorganisms in the pH range of 5 to 7 was determined. A concentration of 0.4% (w/w) caffeic acid was enough to inhibit the growth of some of the studied microorganisms in the pH range of 5 to 7. However, near-neutral pH concentrations higher than 0.4% were needed to inhibit some microorganisms, including Listeria monocytogenes, E. coli, and Staphylococcus aureus, in the medium.
Almajano
M P
MP
Food Engineering and Biotechnology Dept., College of Agricultural Engineering of Barcelona, The Technical Univ. of Catalonia, Av. Canal Olímpic, s/n, 08860 Castelldefels, Barcelona, Spain.
Carbó
R
R
Delgado
M E
ME
Gordon
M H
MH
D000704:Analysis of Variance; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002109:Caffeic Acids / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004355:Drug Stability; D004655:Emulsions; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005079:Excipients / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry; D005519:Food Preservation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D010084:Oxidation-Reduction; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013499:Surface Properties; D013501:Surface-Active Agents
D000900:Anti-Bacterial Agents; D002109:Caffeic Acids; D004655:Emulsions; D005079:Excipients; D005290:Ferric Compounds; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine; C040048:caffeic acid