title: Vertebrate conserved non coding DNA regions have a high persistence length and a short persistence time.
abstract: BACKGROUND
  The comparison of complete genomes has revealed surprisingly large numbers of conserved non-protein-coding (CNC) DNA regions. However, the biological function of CNC remains elusive. CNC differ in two aspects from conserved protein-coding regions. They are not conserved across phylum boundaries, and they do not contain readily detectable sub-domains. Here we characterize the persistence length and time of CNC and conserved protein-coding regions in the vertebrate and insect lineages.
  
  
  RESULTS
  The persistence length is the length of a genome region over which a certain level of sequence identity is consistently maintained. The persistence time is the evolutionary period during which a conserved region evolves under the same selective constraints. Our main findings are: (i) Insect genomes contain 1.60 times less conserved information than vertebrates; (ii) Vertebrate CNC have a higher persistence length than conserved coding regions or insect CNC; (iii) CNC have shorter persistence times as compared to conserved coding regions in both lineages.
  
  
  CONCLUSIONS
  Higher persistence length of vertebrate CNC indicates that the conserved information in vertebrates and insects is organized in functional elements of different lengths. These findings might be related to the higher morphological complexity of vertebrates and give clues about the structure of active CNC elements. Shorter persistence time might explain the previously puzzling observations of highly conserved CNC within each phylum, and of a lack of conservation between phyla. It suggests that CNC divergence might be a key factor in vertebrate evolution. Further evolutionary studies will help to relate individual CNC to specific developmental processes.
authors.affiliation: Computational Cancer Genomics Group, Swiss Institute of Bioinformatics, Lausanne, Switzerland. Dorota.Retelska@isrec.ch
authors.forename: Dorota
authors.initials: D
authors.lastname: Retelska
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Beaudoing
authors.forename: Cédric
authors.initials: C
authors.lastname: Notredame
authors.forename: C Victor
authors.initials: CV
authors.lastname: Jongeneel
authors.forename: Philipp
authors.initials: P
authors.lastname: Bucher
mesh_terms: D000818:Animals; D017124:Conserved Sequence; D021901:DNA, Intergenic / Q000235:genetics*; D004330:Drosophila / Q000235:genetics; D019143:Evolution, Molecular; D016678:Genome / Q000235:genetics*; D049750:Genome, Insect / Q000235:genetics; D006801:Humans; D013997:Time Factors; D014714:Vertebrates / Q000235:genetics*
chemical_list: D021901:DNA, Intergenic