Multipotency and growth characteristic of periosteum-derived progenitor cells for chondrogenic, osteogenic, and adipogenic differentiation.
The mesengenic multipotency of cryopreserved periosteum-derived progenitor cells (PDPCs) for chondrogenesis, osteogenesis and adipogenesis was investigated. Differentiation was verified using RT-PCR and histological analysis. For characterization, FACS analysis was performed with specific surface markers of mesenchymal stem cells (MSCs). Among PDPCs, unsorted periosteum-derived cells (PDCs) and dermal fibroblasts, the most distinct characteristics were found to be CD9, CD105, and CD166. In addition, these markers in PDPCs were continuously maintained until passage 15. We developed a rapid method for the isolation of PDPCs that can differentiate into mesodermal lineages and provide enough cells in a short period of time for allogeneic cell therapy.
Choi
Yong-Soo
YS
Department of Biological Engineering, Inha University, Incheon 402-751, Korea.
Noh
Sang-Eun
SE
Lim
Sang-Min
SM
Lee
Chang-Woo
CW
Kim
Chul-Soo
CS
Im
Moon-Whan
MW
Lee
Moon-Hee
MH
Kim
Dong-Il
DI
D050156:Adipogenesis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D015703:Antigens, CD / Q000032:analysis; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D000071063:Endoglin; D005326:Fetal Proteins / Q000032:analysis; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000032:analysis; D039902:Multipotent Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D010521:Periosteum / Q000166:cytology*; D011956:Receptors, Cell Surface / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D060245:Tetraspanin 29; D020410:Activated-Leukocyte Cell Adhesion Molecule
D015703:Antigens, CD; D015816:Cell Adhesion Molecules, Neuronal; D000071063:Endoglin; D005326:Fetal Proteins; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D060245:Tetraspanin 29; C105613:ALCAM protein, human; D020410:Activated-Leukocyte Cell Adhesion Molecule; C554869:CD9 protein, human; C064206:ENG protein, human