Allergen challenge decreases mRNA expression of regulatory cytokines in whole blood of high-IgE beagles.
The kinetics of cytokine expression was evaluated in whole blood from high-IgE beagles previously sensitized to house dust mites (HDM) and known to develop clinical signs compatible with atopic dermatitis (AD) upon allergen exposure. Six high-IgE beagles were environmentally challenged daily for 3 h on three consecutive days with a HDM solution. Clinical signs were evaluated before, during, and after the conclusion of the challenge (days 0, 2, 4 and 17) and expression analyses of Th2 (IL-4 and IL-13) and regulatory (IL-10 and TGF-beta) cytokine mRNA were undertaken on blood samples at each time point using real-time polymerase chain reaction. Multiple comparison used to detect significant differences in clinical scores and expression levels of cytokine mRNA revealed that the clinical scores on days 2 and 4 were higher than those on days 0 and 17 (P < 0.05) but no temporal differences in the expression levels of IL-4 and IL-13 mRNA. Expression of TGF-beta mRNA was, however, significantly lower on day 4 (P < 0.05) and the expression of IL-10 mRNA on days 4 and 17 was significantly lower than those on days 0 and 2 (P < 0.05). The results indicate that allergen challenge decreases mRNA expression of regulatory cytokines in whole blood without enhanced mRNA expression of Th2 cytokines and suggest aberrant regulatory T-cell function in the immunopathogenesis of AD in high-IgE beagles.
Maeda
Sadatoshi
S
Department of Veterinary Internal Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
Tsuchida
Hiromi
H
Marsella
Rosanna
R
D000485:Allergens / Q000276:immunology*; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis*; D017931:DNA Primers; D003876:Dermatitis, Atopic / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000097:blood / Q000276:immunology*; D004285:Dogs; D005260:Female; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D016753:Interleukin-10 / Q000096:biosynthesis; D018793:Interleukin-13 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008297:Male; D016133:Polymerase Chain Reaction; D039981:Pyroglyphidae / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D012882:Skin Tests / Q000662:veterinary; D016212:Transforming Growth Factor beta / Q000096:biosynthesis
D000485:Allergens; D016207:Cytokines; D017931:DNA Primers; D018793:Interleukin-13; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D015847:Interleukin-4; D007073:Immunoglobulin E