Microarray analysis of thyroid stimulating hormone, insulin-like growth factor-1, and insulin-induced gene expression in FRTL-5 thyroid cells.
To determine which genes are regulated by thyroid stimulating hormone (thyrotropin, TSH), insulin and insulin-like growth factor-1 (IGF-1) in the rat thyroid, we used the microarray technology and observed the changes in gene expression. The expressions of genes for bone morphogenetic protein 6, the glucagon receptor, and cyclin D1 were increased by both TSH and IGF-1; for cytochrome P450, 2c37, the expression was decreased by both. Genes for cholecystokinin, glucuronidase, beta, demethyl-Q 7, and cytochrome c oxidase, subunit VIIIa, were up-regulated; the genes for ribosomal protein L37 and ribosomal protein L4 were down-regulated by TSH and insulin. However, there was no gene observed to be regulated by all three: TSH, IGF-1, and insulin molecules studied. These findings suggest that TSH, IGF-1, and insulin stimulate different signal pathways, which can interact with one another to regulate the proliferation of thyrocytes, and thereby provide additional influence on the process of cellular proliferation.
Lee
You Jin
YJ
Department of Internal Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.
Park
Do Joon
DJ
Shin
Chan Soo
CS
Park
Kyong Soo
KS
Kim
Seong Yeon
SY
Lee
Hong Kyu
HK
Park
Young Joo
YJ
Cho
Bo Youn
BY
D000818:Animals; D055418:Bone Morphogenetic Protein 6; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis; D045744:Cell Line, Tumor; D019938:Cyclin D1 / Q000096:biosynthesis; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007328:Insulin / Q000096:biosynthesis* / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis*; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D018027:Receptors, Glucagon / Q000096:biosynthesis; D013961:Thyroid Gland / Q000378:metabolism*; D013972:Thyrotropin / Q000096:biosynthesis* / Q000378:metabolism; D013997:Time Factors
C525818:Bmp6 protein, rat; D055418:Bone Morphogenetic Protein 6; D019485:Bone Morphogenetic Proteins; D007328:Insulin; D018027:Receptors, Glucagon; D019938:Cyclin D1; D007334:Insulin-Like Growth Factor I; D013972:Thyrotropin