title: Cells tracking in a live zebrafish embryo.
abstract: We designed a set of procedures for achieving the tracking of cell nuclei and the identification of cell divisions in live zebrafish embryos using 3D+time images acquired by confocal laser scanning microscopy (CLSM). Our strategy includes image signal enhancement with feature preserving denoising algorithm, automated identification of the nuclei position, extraction of the optical flow from 3D images sequences and tracking of nuclei.
authors.affiliation: Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina and Dipartimento di Elettronica, Informatica e Sistemistica, Università di Bologna, Italy. camilo@dc.uba.ar
authors.forename: Camilo
authors.initials: C
authors.lastname: Melani
authors.forename: Matteo
authors.initials: M
authors.lastname: Campana
authors.forename: Benoit
authors.initials: B
authors.lastname: Lombardot
authors.forename: Barbara
authors.initials: B
authors.lastname: Rizzi
authors.forename: Federico
authors.initials: F
authors.lastname: Veronesi
authors.forename: Cecilia
authors.initials: C
authors.lastname: Zanella
authors.forename: Paul
authors.initials: P
authors.lastname: Bourgine
authors.forename: Karol
authors.initials: K
authors.lastname: Mikula
authors.forename: Nadine
authors.initials: N
authors.lastname: Peyriéras
authors.forename: Alessandro
authors.initials: A
authors.lastname: Sarti
mesh_terms: D000465:Algorithms; D000818:Animals; D001185:Artificial Intelligence; D004625:Embryo, Nonmammalian / Q000166:cytology*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015027:Zebrafish / Q000033:anatomy & histology* / Q000196:embryology*