title: Costimulatory effects of complement receptor type 3 and Fc receptor for IgG (FcgammaR) on superoxide production and signal transduction in bovine neutrophils.
abstract: The present study evaluated the costimulatory effects of complement receptor type 3 (CR3) and Fc receptor for IgG (FcgammaR) on superoxide production and intracellular signal transduction in bovine neutrophils. Stimulation with opsonized zymosan (OPZ) and heat-aggregated bovine IgG (Agg-IgG) resulted in much greater superoxide production and chemiluminescent (CL) responses in normal neutrophils compared with those stimulated with OPZ or Agg-IgG only. Superoxide production and CL response were closely associated with the stimulant-induced rise of the intracellular calcium ([Ca2+]i) concentration, amount of tyrosine phosphorylated 100 kDa protein, and activation of p38 mitogen-activated protein kinase (p38 MAPK). No costimulatory effect was found for these receptors on superoxide production in CR3-deficient neutrophils. Costimulation of CR3 and FcgammaR on bovine neutrophils leads to enhancement of superoxide production and their signaling pathways and appears to be associated with enhancement of neutrophil functions.
authors.affiliation: Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan. nagahata@rakuno.ac.jp
authors.forename: Hajime
authors.initials: H
authors.lastname: Nagahata
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Higuchi
authors.forename: Osamu
authors.initials: O
authors.lastname: Inanami
authors.forename: Mikinori
authors.initials: M
authors.lastname: Kuwabara
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D011951:Receptors, Complement / Q000378:metabolism*; D017452:Receptors, IgG / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism*; D015054:Zymosan / Q000494:pharmacology
chemical_list: D011951:Receptors, Complement; D017452:Receptors, IgG; D013481:Superoxides; D015054:Zymosan