title: In vivo dynamic light scattering imaging of blood coagulation.
abstract: Physiological blood coagulation/clotting is an essential biological process that is initiated by vessel injury and includes a cascade of enzymatic reactions finalized by fibrin polymerization and clot formation. We utilize dynamic light scattering (DLS) imaging to monitor in vivo red cell mobility as an indicator of blood coagulation. In the course of the experiments, blood flow is arrested using mechanical occlusion, and then laser injury is applied. We demonstrate that the combination of laser injury with DLS imaging on occluded blood vessels (i.e., under static conditions) is suitable to detect even subtle changes of plasma viscosity in the circulatory system, which reflects the process of clot development. This approach is noninvasive and has a relatively simple and easy-to-use technical design. Thus, the proposed methodology provides a promising tool for investigating blood clotting within the vasculature.
authors.affiliation: Weizmann Institute of Science, Department of Veterinary Resources, Rehovot 76100, Israel.
authors.forename: Vyacheslav
authors.initials: V
authors.lastname: Kalchenko
authors.forename: Alexander
authors.initials: A
authors.lastname: Brill
authors.forename: Michael
authors.initials: M
authors.lastname: Bayewitch
authors.forename: Ilya
authors.initials: I
authors.lastname: Fine
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zharov
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Galanzha
authors.forename: Valery
authors.initials: V
authors.lastname: Tuchin
authors.forename: Alon
authors.initials: A
authors.lastname: Harmelin
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000502:physiology*; D001780:Blood Coagulation Tests / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007834:Lasers; D051379:Mice; D012031:Refractometry / Q000379:methods*; D012542:Scattering, Radiation