title: Molecular identification of Cryptosporidium spp. from fecal samples of felines, canines and bovines in the state of São Paulo, Brazil.
abstract: The objective of this study was to obtain information of epidemiological nature through genotypic characterization of Cryptosporidium isolates from dogs, cats and bovines from the state of São Paulo, Brazil. The extraction of DNA from oocysts was carried out and polymerase chain reaction was accomplished using specific primers to 18S rRNA gene. The amplicons were directed sequenced. Seven cat samples, nine dog samples and nine bovine samples were analysed. From the seven cat samples the genotypic analyses revealed Cryptosporidium felis in all. These were the first genotypic characterization of Cryptosporidium from domestic felines in Brazil. In nine sequenced samples from dogs, genotypic identities compatible with Cryptosporidium canis were revealed in all samples. The genotypic analyses in bovines revealed Cryptosporidium parvum in eight samples and Cryptosporidium bovis in another sample, the last one being a non-zoonotic species, not related to clinical symptoms and described for the first time in Brazil.
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Cidade Universitária, CEP 05508-270, São Paulo, SP, Brazil.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Thomaz
authors.forename: Marcelo V
authors.initials: MV
authors.lastname: Meireles
authors.forename: Rodrigo M
authors.initials: RM
authors.lastname: Soares
authors.forename: Hilda F J
authors.initials: HF
authors.lastname: Pena
authors.forename: Solange M
authors.initials: SM
authors.lastname: Gennari
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002371:Cat Diseases / Q000453:epidemiology / Q000469:parasitology; D002415:Cats; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016785:Cryptosporidium parvum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology; D004285:Dogs; D005243:Feces / Q000469:parasitology*; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D016415:Sequence Alignment; D013045:Species Specificity
chemical_list: D016054:DNA, Protozoan