Lipid bilayer microarray for parallel recording of transmembrane ion currents.
This paper describes a multiwell biochip for simultaneous parallel recording of ion current through transmembrane pores reconstituted in planar lipid bilayer arrays. Use of a thin poly(p-xylylene) (parylene) film having micrometer-sized apertures (phi=15-50 microm, t=20 microm) led to formation of highly stable bilayer lipid membranes (BLMs) for incorporation of transmembrane pores; thus, a large number of BLMs could be arrayed without any skillful technique. We optically confirmed the simultaneous formation of BLMs in a 5x5 matrix, and in our durability test, the BLM lasted more than 15 h. Simultaneous parallel recording of alamethicin and gramicidin transmembrane pores in multiple contiguous recording sites demonstrated the feasibility of high-throughput screening of transmembrane ion currents in artificial lipid bilayers.
Le Pioufle
Bruno
B
LIMMS, CNRS-IIS, and CIRMM, IIS, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505 Japan.
Suzuki
Hiroaki
H
Tabata
Kazuhito V
KV
Noji
Hiroyuki
H
Takeuchi
Shoji
S
D000408:Alamethicin / Q000494:pharmacology; D004553:Electric Conductivity; D004566:Electrodes; D006096:Gramicidin / Q000494:pharmacology; D015640:Ion Channel Gating / Q000187:drug effects; D007473:Ion Channels / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry*; D008567:Membranes, Artificial; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D055095:Optics and Photonics; D011108:Polymers / Q000737:chemistry; D014992:Xylenes / Q000737:chemistry
D007473:Ion Channels; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D011108:Polymers; D014992:Xylenes; D006096:Gramicidin; C011055:parylene; D000408:Alamethicin