Hevea latex lectin binding protein in C-serum as an anti-latex coagulating factor and its role in a proposed new model for latex coagulation.
A distinct protein specifically recognized by its strong interaction with Hevea latex lectin (HLL) was detected in the aqueous C-serum fraction of centrifuged fresh latex. This C-serum lectin binding protein (CS-HLLBP) exhibited strong inhibition of HLL-induced hemagglutination. The CS-HLLBP was purified to homogeneity by a protocol that included ammonium sulfate fractionation, size exclusion and ion exchange chromatography. The purified CS-HLLBP had a specific HI titer of 0.23microg ml(-1). Its M(r)s analyzed by SDS-PAGE was ca. 40kDa and that by gel filtration was ca. 204kDa. It has a pI value of 4.7, an optimum activity between pH 6 and10 and was heat stable up to 50 degrees C. The HI activity of CS-HLLBP was abolished upon treatment with chitinase. The CS-HLLBP inhibited HLL-induced rubber particle aggregation in a dose dependent manner. A highly positive correlation between CS-HLLBP activity and rubber yield per tapping was found. The correlations for fresh latex (r=0.98, P<0.01) and dry rubber (r=0.95, P<0.01) were both highly significant. This indicated that the CS-HLLBP might be used as a reliable marker for the mass screening of young seedlings to identify and select clones with potential to be superior producers of rubber. A latex anti-coagulating role of the CS-HLLBP is proposed. The findings described in this 3 paper series have been used to propose a new model of rubber latex coagulation that logically describes roles for the newly characterized latex lectin and the two lectin binding proteins.
Wititsuwannakul
Rapepun
R
Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. rapepun.w@psu.ac.th
Pasitkul
Piyaporn
P
Jewtragoon
Pattavuth
P
Wititsuwannakul
Dhirayos
D
D028482:Hevea / Q000737:chemistry / Q000502:physiology*; D007840:Latex / Q000737:chemistry / Q000378:metabolism*; D020315:Latex Hypersensitivity / Q000378:metabolism*; D008954:Models, Biological; D037121:Plant Lectins / Q000737:chemistry* / Q000502:physiology*; D010940:Plant Proteins / Q000737:chemistry / Q000502:physiology*; D011485:Protein Binding; D012680:Sensitivity and Specificity; D012995:Solubility; D014461:Ultracentrifugation
D007840:Latex; D037121:Plant Lectins; D010940:Plant Proteins