Wash-free in-situ self-desalting and peptide enrichment by block copolymer analyzed with MALDI-TOFMS.
A novel technique of simultaneous peptide enrichment and wash-free in-situ self-desalting for MALDI analysis is reported, where a newly synthesized block copolymer with a microphase-separated configuration is applied to embed salts with its hydrophilic domain of poly(ethylene oxide) and concentrate peptides with its hydrophobic domain of polysulfone.
Zhang
Ying
Y
Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, PR China.
Fang
Jianhua
J
Kuang
Yongbo
Y
Guo
Xiaoxia
X
Lu
Haojie
H
Yang
Pengyuan
P
D001639:Bicarbonates / Q000737:chemistry; D002793:Cholic Acids / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013450:Sulfones / Q000737:chemistry*
D001639:Bicarbonates; D002793:Cholic Acids; D009211:Myoglobin; D010455:Peptides; D013450:Sulfones; C406278:poly(ethylene oxide)-polysulfone block copolymer; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate