[Inhibitory effect of Chinese herbal medicine Xin-Kang oral liquid on replication of CVB3 RNA in Coxsackievirus B3 infected mice].
OBJECTIVE
To investigate the CVB3 RNA concentration in myocardial tissues and inhibitory effect of Chinese herbal medicine Xin-Kang oral liquid on viral RNA replication in Coxsackievirus B3 infected myocardium.


METHODS
The total RNA was extracted from the infected murine myocardium, the reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect enterovirus RNA concentration in infected mice with Coxsackievirus B3 gene band. We used "Gel works system" to scan the electrophoresis image to detect CVB3 gene band.


RESULTS
The mean concentration of myocardial CVB3 RNA of Xin-Kang oral liquid treated groups was markedly lower than that of virus control group (P>0.01), but the CVB3 RNA in myocardial tissues has not been destroyed by Xin-Kang oral liquid feed in different phase.


CONCLUSIONS
Chinese herbal medicine Xin-Kang oral liquid could inhibit CVB3 RNA replication in myocardial tissue, but it did not destruct the virus.
Wan
Su-Jun
SJ
Guang An Men Hospital, The Chinese Academy of Traditional Chinese Medicine, Beijing, China. wansj@163.com
Yang
Fan
F
Huang
Xia-Zhen
XZ
D000818:Animals; D003384:Coxsackievirus Infections / Q000188:drug therapy*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D029822:Enterovirus B, Human / Q000187:drug effects* / Q000235:genetics; D051379:Mice; D009205:Myocarditis / Q000188:drug therapy / Q000821:virology; D008517:Phytotherapy; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016896:Treatment Outcome; D014779:Virus Replication / Q000187:drug effects* / Q000235:genetics
D004365:Drugs, Chinese Herbal; D012367:RNA, Viral; C534050:xin-kang