title: Up-regulation of intelectin in sheep after infection with Teladorsagia circumcincta.
abstract: A novel intelectin molecule designated sheep intelectin 2 (sITLN2) was detected in sheep abomasal mucosa. The full sequence shared 76-83% homology with other mammalian intelectins. Intelectins are mucus-associated proteins that have been shown to be up-regulated in gastrointestinal nematode infections in rodents and in human asthma. Expression of sheep abomasal ITLN2 mRNA was significantly up-regulated on day 10 post-challenge of worm-free sheep with Teladorsagia circumcincta and at day 2 in previously infected, immune sheep. Increased expression of ITLN protein following challenge was confirmed by Western blot and was immunolocalised to the mucous neck cells of the abomasal mucosa. Infection with T. circumcincta was also associated with increased levels of abomasal transcripts encoding sheep mast cell protease-1, ovine galectin-14 and IL4, which collectively suggested a Th2 type response. Intelectin may play an important role in the mucosal response to gastrointestinal nematode infections in ruminants.
authors.affiliation: Division of Veterinary Clinical Sciences, University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK. anne.french@ed.ac.uk
authors.forename: Anne T
authors.initials: AT
authors.lastname: French
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Knight
authors.forename: W David
authors.initials: WD
authors.lastname: Smith
authors.forename: Jeremy K
authors.initials: JK
authors.lastname: Brown
authors.forename: Nicola M
authors.initials: NM
authors.lastname: Craig
authors.forename: Judith A
authors.initials: JA
authors.lastname: Pate
authors.forename: Hugh R P
authors.initials: HR
authors.lastname: Miller
authors.forename: Alan D
authors.initials: AD
authors.lastname: Pemberton
mesh_terms: D000018:Abomasum / Q000276:immunology* / Q000469:parasitology; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western / Q000379:methods; D053818:Chymases / Q000235:genetics / Q000378:metabolism; D005260:Female; D037161:Galectins / Q000235:genetics / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism / Q000469:parasitology; D006790:Host-Parasite Interactions; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D007411:Intestinal Diseases, Parasitic / Q000276:immunology*; D008969:Molecular Sequence Data; D009349:Nematode Infections / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*; D015854:Up-Regulation
chemical_list: D037161:Galectins; D012333:RNA, Messenger; D015847:Interleukin-4; D053818:Chymases