Novel Brucella strain (BO1) associated with a prosthetic breast implant infection.
We report the microbiological, biochemical, and molecular characterization of an unusual Brucella strain (BO1) isolated from a breast implant wound in a 71-year-old woman with clinical symptoms consistent with brucellosis. Initial phenotypic analysis, including biochemical and antimicrobial susceptibility testing, cellular fatty acid analysis, and molecular analysis based on DNA-DNA reassociation and the presence of multiple copies of IS711 element suggested that the isolate was a Brucella-like organism, but species determination using microbiological algorithms was unsuccessful. Furthermore, molecular data based on 16S rRNA gene sequencing and multilocus sequence analysis demonstrated that BO1 was an unusual Brucella strain and not closely related to any currently described Brucella species. However, comparison with equivalent sequences in Ochrobactrum spp. confirms that the isolate is much more closely related to Brucella than to Ochrobactrum spp., and thus the isolate likely represents an atypical and novel strain within the genus Brucella.
De
Barun K
BK
Centers for Disease Control and Prevention, Mail Stop G34, 1600 Clifton Rd., Atlanta, GA 30333, USA. bkd1@cdc.gov
Stauffer
Larry
L
Koylass
Mark S
MS
Sharp
Susan E
SE
Gee
Jay E
JE
Helsel
Leta O
LO
Steigerwalt
Arnold G
AG
Vega
Robert
R
Clark
Thomas A
TA
Daneshvar
Maryam I
MI
Wilkins
Patricia P
PP
Whatmore
Adrian M
AM
D000368:Aged; D015373:Bacterial Typing Techniques; D018427:Breast Implants / Q000382:microbiology*; D002002:Brucella / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002006:Brucellosis / Q000382:microbiology* / Q000503:physiopathology; D016000:Cluster Analysis; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D042041:Ochrobactrum / Q000235:genetics; D010802:Phylogeny; D016459:Prosthesis-Related Infections / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D005227:Fatty Acids