The use of the Vibrio harveyi luminescence mutagenicity assay as a rapid test for preliminary assessment of mutagenic pollution of marine sediments.
Mutagenic pollution of the natural environment is currently one of the most serious environmental problems. It includes the pollution of marine sediments. Therefore, rapid detection of the presence of mutagens is an important issue. Recently, we have developed a novel microbiological assay for rapid assessment of mutagenicity of samples from the natural environment. This assay is based on bioluminescence of a mutant Vibrio harveyi strain, and was shown to be useful in testing samples of marine water and plant tissues. Here we demonstrate the usefulness of this assay in preliminary assessment of mutagenic pollution of marine sediments. Mutagenicity of environmental samples taken from the Baltic Sea, is documented and compared here with a commercially available standard sediment sample (IAEA 383), which contains known amounts of mutagenic compounds. The whole procedure, from obtaining a sample in the laboratory to getting final results, is very short (less than 4 h).
Podgórska
Beata
B
Institute of Oceanology, Polish Academy of Sciences, Sopot, Poland.
Pazdro
Ksenia
K
Wegrzyn
Grzegorz
G
D004785:Environmental Pollutants / Q000032:analysis / Q000633:toxicity*; D019015:Geologic Sediments / Q000737:chemistry*; D049449:Luminescence; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000032:analysis / Q000633:toxicity*; D012623:Seawater / Q000737:chemistry*; D014733:Vibrio / Q000187:drug effects* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
D004785:Environmental Pollutants; D009153:Mutagens; D014874:Water Pollutants, Chemical