Supramolecular architectures of streptavidin on biotinylated self-assembled monolayers. Tracking biomolecular reorganization after bioconjugation.
In this work we have studied the supramolecular bioconjugation of streptavidin (SAv) on biotinylated self-assembled monolayers. By using the quartz crystal microbalance technique with dissipation we were able to follow in real time the biomolecular reorganization within the film. The overall process could be described as an early stage involving a significant increase in surface coverage followed by another stage where the SAv layer slowly reached the asymptotic coverage. Finally, a reorganization process takes place in the bioconjugated film. These results on the kinetics of biomolecular reorganization can be described in terms of the Lifshitz-Slyozov law. These are the first experimental results demonstrating the complexity and the different time scales involved on the bioconjugation of SAv at solid-liquid interfaces. We consider that these findings could have strong implications on the molecular design of biosensing platforms.
Azzaroni
Omar
O
Max-Planck-Institut für Polymerforschung Ackermannweg 10, 55128 Mainz, Germany. azzaroni@mpip-mainz.mpg.de
Mir
Monica
M
Knoll
Wolfgang
W
D001710:Biotin / Q000737:chemistry*; D007700:Kinetics; D019809:Streptavidin / Q000737:chemistry*; D020349:Surface Plasmon Resonance
D001710:Biotin; D019809:Streptavidin