title: Detection of Dialister pneumosintes in the subgingival biofilm of subjects with periodontal disease.
abstract: Dialister pneumosintes has been indicated as a potentially new periodontopathic species. This study evaluated the prevalence of this microorganism in saliva and subgingival biofilm from subjects with different periodontal conditions. Subgingival biofilm and saliva samples from 48 subjects with periodontal health (PH) and 116 patients with chronic periodontitis (CP) were obtained. DNA was extracted from the samples and the presence of D. pneumosintes was determined by PCR. Differences in clinical parameters and frequency of D. pneumosintes between groups were sought by Mann-Whitney, Chi-square and Fisher's exact tests. Overall, D. pneumosintes was detected in 47.8% of the biofilm samples, but only in 3% of saliva samples. CP patients presented a significantly greater mean prevalence of this species in sites with periodontal health and periodontal infection (43.5+/-7.4% and 62.1+/-6.4%, respectively) than PH subjects (29.4+/-7.9%) (Mann-Whitney; p<0.01). Moreover, significant associations between the prevalence of D. pneumosintes and pocket depth (p=0.001), attachment loss (p=0.001) and bleeding on probing (GLM, p=0.014) were observed after adjusting for age and gender. These findings corroborate the association of D. pneumosintes with periodontitis.
authors.affiliation: Dental School, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Cíntia Tereza Lima
authors.initials: CT
authors.lastname: Ferraro
authors.forename: Camila
authors.initials: C
authors.lastname: Gornic
authors.forename: Adriana Sampaio
authors.initials: AS
authors.lastname: Barbosa
authors.forename: Rafael J Marques
authors.initials: RJ
authors.lastname: Peixoto
authors.forename: Ana Paula Vieira
authors.initials: AP
authors.lastname: Colombo
mesh_terms: D000328:Adult; D018441:Biofilms / Q000254:growth & development*; D004269:DNA, Bacterial / Q000032:analysis; D005881:Gingiva / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D010510:Periodontal Diseases / Q000382:microbiology*; D010514:Periodontal Pocket / Q000382:microbiology; D016133:Polymerase Chain Reaction; D045854:Veillonellaceae / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D004269:DNA, Bacterial