title: Characterization of teleost phagocyte NADPH oxidase: molecular cloning and expression analysis of carp (Cyprinus carpio) phagocyte NADPH oxidase.
abstract: We previously demonstrated that some fish have unique response in the form of reactive oxygen species (ROS) production. In the present study, we cloned and sequenced the full-length cDNAs for carp (Cyprinus carpio) phagocyte NADPH oxidase components: gp91phox, p22phox, p47phox, p67phox and p40phox. These amino acid sequences were compared with other teleost and mammalian homologues, to elucidate the features of ROS production of fish neutrophils. The phylogeny analysis clearly demonstrates that the radiation of phagocyte oxidase components took place in the common ancestor of teleosts and mammals. Thereafter, the overall structure and expression pattern of phagocyte oxidase have been highly conserved in two different strains. However the amino acid identity of p67phox and p47phox was relatively lower than the amino acid identities of other components. Moreover, a synteny analysis supports the hypothesis that there was strong selective pressure in the p67phox and p47phox genes. Thus, it is likely that the higher divergence of p67phox and p47phox are responsible for the difference of ROS responses between different species of teleosts.
authors.affiliation: Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252-8510, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Mayumi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takeda
authors.forename: Masataka
authors.initials: M
authors.lastname: Hoshiko
authors.forename: Ken
authors.initials: K
authors.lastname: Serada
authors.forename: Michihiro
authors.initials: M
authors.lastname: Murata
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Moritomo
authors.forename: Fumio
authors.initials: F
authors.lastname: Takizawa
authors.forename: Isao
authors.initials: I
authors.lastname: Kobayashi
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Araki
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Hideki
authors.initials: H
authors.lastname: Sumimoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D007962:Leukocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism*; D010586:Phagocytes / Q000201:enzymology*; D010802:Phylogeny
chemical_list: D019255:NADPH Oxidases