title: Oligonucleotide loading determines cellular uptake of DNA-modified gold nanoparticles.
abstract: The cellular internalization of oligonucleotide-modified nanoparticles is investigated. Uptake is dependent on the density of the oligonucleotide loading on the surface of the particles, where higher densities lead to greater uptake. Densely functionalized nanoparticles adsorb a large number of proteins on the nanoparticle surface. Nanoparticle uptake is greatest where a large number of proteins are associated with the particle.
authors.affiliation: Department of Chemistry and International Institute for Nanotechnology, Northwestern University, Evanston, IL 60208-3113, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Giljohann
authors.forename: Dwight S
authors.initials: DS
authors.lastname: Seferos
authors.forename: Pinal C
authors.initials: PC
authors.lastname: Patel
authors.forename: Jill E
authors.initials: JE
authors.lastname: Millstone
authors.forename: Nathaniel L
authors.initials: NL
authors.lastname: Rosi
authors.forename: Chad A
authors.initials: CA
authors.lastname: Mirkin
mesh_terms: D001692:Biological Transport; D004247:DNA / Q000737:chemistry* / Q000493:pharmacokinetics; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000493:pharmacokinetics; D013499:Surface Properties
chemical_list: D009838:Oligodeoxyribonucleotides; D006046:Gold; D004247:DNA