Measurement of the sugar-binding specificity of lectins using multiplexed bead-based suspension arrays.
A multiplexed glyco-bead array method for determining the sugar-binding specificities of plant lectins was described by a bead-based flow cytometric assay. Glycopeptides with N- and O-glycans were immobilized on multiplexed beads, and the specificities of several kinds of sugar chains were measured in a single reaction. This strategy is easy, rapid, reproducible, and suitable for small samples, and allows the reliable elucidation of sugar-binding properties of lectins under identical conditions.
Yamamoto
Kazuo
K
Frontier Science Bioscience, University of Tokyo, Kashiwa Chiba, Japan.
Yasukawa
Fumiko
F
Ito
Seiichiro
S
D000818:Animals; D050260:Carbohydrate Metabolism; D002417:Cattle; D005434:Flow Cytometry; D006020:Glycopeptides / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D046228:Microarray Analysis / Q000379:methods*; D008863:Microspheres; D013379:Substrate Specificity; D013552:Swine
D006020:Glycopeptides; D037102:Lectins