Identification and isolation of synovial dendritic cells.
In rheumatoid arthritis patients, three compartments need to be considered: peripheral blood, synovial fluid, and synovial tissue. Dendritic cells characterized from each compartment have different properties. The methods given are based on cell sorting for isolation of cells, and flow cytometry and immunohistochemical staining for analysis of cells in these compartments.
Pettit
Allison R
AR
Centre for Immunology and Cancer Research, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
Cavanagh
Lois
L
Boyce
Amanda
A
Padmanabha
Jagadish
J
Peng
Judy
J
Thomas
Ranjeny
R
D000818:Animals; D039521:CD11c Antigen / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D003713:Dendritic Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D053650:Interleukin-3 Receptor alpha Subunit / Q000378:metabolism; D016185:Receptors, Interleukin-3 / Q000378:metabolism; D013582:Synovial Fluid / Q000166:cytology* / Q000276:immunology
D039521:CD11c Antigen; C115022:IL3RA protein, human; D053650:Interleukin-3 Receptor alpha Subunit; D016185:Receptors, Interleukin-3