Protein-protein interactions within type III secretion system-dependent pili of Rhizobium sp. strain NGR234.
Pili synthesized by the type III secretion system of Rhizobium species strain NGR234 are essential for protein secretion and thus for efficient symbiosis with many legumes. Isolation and partial purification of these pili showed that they are composed of at least three proteins, NopA, NopB, and NopX. Using biochemical assays, we show here that these proteins interact directly with one another.
Saad
Maged M
MM
LBMPS, Université de Genève, Sciences III, 30 quai Ernest-Ansermet, 1211 Genève 4, Switzerland.
Staehelin
Christian
C
Broughton
William J
WJ
Deakin
William J
WJ
D002850:Chromatography, Gel; D036781:Fimbriae Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000737:chemistry*; D008954:Models, Biological; D008970:Molecular Weight; D011485:Protein Binding; D012231:Rhizobium / Q000737:chemistry* / Q000378:metabolism*
D036781:Fimbriae Proteins