Placenta-derived, cellular messenger RNA expression in the maternal blood of preeclamptic women.
OBJECTIVE
To perform gene expression profiling and real-time quantitative reverse-transcription polymerase chain reaction (PCR) analysis to identify biomarkers of preeclampsia in cellular messenger RNA (mRNA) from maternal blood.


METHODS
We performed a microarray analysis with five maternal blood samples from women with preeclampsia and five matched control subjects. Up-regulated gene expression was further analyzed through reverse-transcription PCR analysis with 28 consecutive blood samples from women affected with preeclampsia and 29 controls.


RESULTS
Both pregnancy-specific beta1 glycoprotein and trophoblast glycoprotein were selected based on microarray analysis. Reverse-transcription PCR analysis detected significantly increased mRNA concentrations among women in the preeclampsia group. When stratified according to mild or severe preeclampsia, 19.2-fold and 41.8-fold increases in pregnancy-specific beta1 glycoprotein and 8.3-fold and 10.6-fold increases in trophoblast glycoprotein were observed, respectively. Among women with hemolysis, elevated liver enzymes, and low platelet count syndrome, 51.6-fold and 13.1-fold increases in pregnancy-specific beta1 glycoprotein and trophoblast glycoprotein were observed, respectively. In the preeclampsia group, pregnancy-specific beta1 glycoprotein correlated with severity of proteinuria (P<.001) and systolic blood pressure (P=.01).


CONCLUSIONS
The mRNA expression of pregnancy-specific beta1 glycoprotein and trophoblast glycoprotein is up-regulated in cells circulating within blood from women with preeclampsia, and pregnancy-specific beta1 glycoprotein expression is positively correlated with the clinical severity of preeclampsia.


METHODS
II.
Okazaki
Shiho
S
Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
Sekizawa
Akihiko
A
Purwosunu
Yuditiya
Y
Farina
Antonio
A
Wibowo
Noroyono
N
Okai
Takashi
T
D000293:Adolescent; D000328:Adult; D015415:Biomarkers; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010920:Placenta / Q000378:metabolism; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation
D015415:Biomarkers; D008562:Membrane Glycoproteins; D011268:Pregnancy-Specific beta 1-Glycoproteins; D012333:RNA, Messenger; C063709:trophoblastic glycoprotein 5T4, human