Peak separation by adventitious or added water in normal-phase chiral HPLC.
Trace amounts of water in eluents for normal-phase chiral HPLC can affect peak retention time, tailing, and resolution. Adventitious water can cause irreproducible analyses. Deliberate addition of water to the eluent can improve peak resolution and save analysis time and solvent needs.
Hintermann
Lukas
L
Institut für Organische Chemie, RWTH Aachen, Landoltweg 1, D-52074 Aachen, Germany. lukas.hintermann@oc.rwth-aachen.de