title: The insecticidal crystal protein Cry2Ab10 from Bacillus thuringiensis: cloning, expression, and structure simulation.
abstract: The cry2Ab-type gene was cloned from Bacillus thuringiensis and designated as cry2Ab10. The recombinant Cry2Ab10 protein expressed in E. coli cells shows high toxicity against Plutella xylostella. The protein structure was constructed by homology modeling, and the receptor-binding sites were predicted by a molecular docking method.
authors.affiliation: Department of Bioengineering and Biotechnology, HuaQiao University, Key Laboratory of Industrial Biotechnology of Fujian Province Universities, Quanzhou, Fujian, China. lyhxm@hqu.edu.cn
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.forename: Guangwei
authors.initials: G
authors.lastname: Fang
authors.forename: Fuying
authors.initials: F
authors.lastname: Cai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000633:toxicity; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000633:toxicity; D003001:Cloning, Molecular; D004731:Endotoxins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000633:toxicity; D015870:Gene Expression; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000633:toxicity; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009036:Moths; D010572:Pest Control, Biological; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D016415:Sequence Alignment
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis