Production of monoclonal antibodies against Neisseria meningitidis using popliteal lymph nodes and in vivo/in vitro immunization: prevalence study of new monoclonal antibodies in greater São Paulo, Brazil.
A rapid and efficient method for preparing monoclonal antibody (MAb) serotypes using Neisseria meningitidis outer membrane were used in BALB/c mouse footpads for the immunization. The popliteal lymph nodes were isolated 19 days later for MAb-producing hybridomas, from which the MAbs against the 37 kDa protein were screened. Variations in class 2/3 (PorB) proteins form the basis for meningococcal serotyping. This is the first report on the preparation of MAbs against N. meningitidis that is specific to PorB protein using popliteal lymph nodes. The new monoclonal antibodies were specific for PorB outer membrane protein FL24(PL)Br, a new serotype 24 class 3 antigens of non-typeable (NT:NST) serogroup B strain, and FL14(PL)Br specific for the serotype 14, and reacted with the S3446 reference strain analyzed. A total of 12% of the case isolates reacted with one or more of the monoclonal antibodies. The high-affinity MAbs produced by hybridoma methodology provide a basis for further research on the pathogenesis and early diagnosis of meningococcus.
Belo
Elza F T
EF
Immunology Section, Adolfo Lutz Institute, São Paulo, Brazil.
Ferraz
Aline S
AS
Coutinho
Ligia M C C
LM
Oliveira
Ana P
AP
Carmo
Andréia M S
AM
Tunes
Claudia F
CF
Ferreira
Tatiane
T
Ito
Andre Y
AY
Machado
Marta S F
MS
De L Franco
Daniele
D
De Gaspari
Elizabeth N
EN
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000627:therapeutic use; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000627:therapeutic use; D016470:Bacteremia / Q000453:epidemiology / Q000276:immunology / Q000628:therapy; D001938:Brazil; D005260:Female; D006801:Humans; D007114:Immunization; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism*; D008589:Meningococcal Infections / Q000453:epidemiology / Q000276:immunology / Q000628:therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D009345:Neisseria meningitidis / Q000276:immunology*; D015995:Prevalence
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal