A new assay for promoter analysis in Chlamydomonas reveals roles for heat shock elements and the TATA box in HSP70A promoter-mediated activation of transgene expression.
The aim of this work was to identify cis-regulatory sequences within the Chlamydomonas HSP70A promoter that mediate its stimulatory effect on the expression of downstream promoters. For this, we deleted/mutated the HSP70A promoter and, using a new assay, quantified its stimulatory effect. Our results indicate that the effect is mediated largely by heat shock elements and the TATA box.
Lodha
Mukesh
M
Institute of Biology II, Plant Biochemistry, University of Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany.
Schulz-Raffelt
Miriam
M
Schroda
Michael
M
D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D018869:Heat-Shock Response; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion; D016385:TATA Box; D014158:Transcription, Genetic; D019076:Transgenes / Q000502:physiology*
D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger