title: Effects of maternal administration of vitamins C and E on ethanol neurobehavioral teratogenicity in the guinea pig.
abstract: Consumption of ethanol during human pregnancy can produce a wide spectrum of teratogenic effects, including neurobehavioral dysfunction. This study, in the guinea pig, tested the hypothesis that chronic maternal administration of antioxidant vitamins C plus E, together with ethanol, mitigates ethanol neurobehavioral teratogenicity. Pregnant guinea pigs received one of the following four chronic oral regimens: ethanol and vitamins C plus E; ethanol and vitamin vehicle; isocaloric-sucrose/pair-feeding and vitamins C plus E; or isocaloric-sucrose/pair-feeding and vehicle. Vitamins C (250 mg) plus E (100mg) or vehicle were given daily, and ethanol (4 g/kg maternal body weight/day) (E) or isocaloric-sucrose/pair-feeding was given for 5 consecutive days followed by 2 days of no treatment each week throughout gestation. One neonate from selected litters was studied on postnatal day (PD) 0. Neurobehavioral function was determined by measuring task acquisition and task retention using an 8-day moving-platform version of the Morris water-maze task, starting on PD 45. Thereafter, in vivo electrophysiologic assessment of changes in hippocampal synaptic plasticity was conducted. There was an ethanol-induced decrease in neonatal brain weight compared with sucrose. The vitamins C plus E regimen protected hippocampal weight relative to brain weight in ethanol offspring, and mitigated the ethanol-induced deficit in the task-retention component of the water-maze task. However, in the sucrose group, this Vit regimen produced deficits in both task acquisition and task retention. The vitamins C plus E regimen did not mitigate the ethanol-induced impairment of hippocampal long-term potentiation. These results indicate that maternal administration of this high-dose vitamins C plus E regimen throughout gestation has limited efficacy and potential adverse effects as a therapeutic intervention for E neurobehavioral teratogenicity.
authors.affiliation: Department of Pharmacology & Toxicology, Queen's University, Kingston, Ontario K7L 3N6, Canada.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Nash
authors.forename: Ferda
authors.initials: F
authors.lastname: Ibram
authors.forename: Hans C
authors.initials: HC
authors.lastname: Dringenberg
authors.forename: James N
authors.initials: JN
authors.lastname: Reynolds
authors.forename: James F
authors.initials: JF
authors.lastname: Brien
mesh_terms: D020268:Alcohol-Induced Disorders, Nervous System / Q000196:embryology / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control* / Q000523:psychology; D000818:Animals; D000831:Animals, Newborn; D001205:Ascorbic Acid / Q000009:adverse effects / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects*; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D006168:Guinea Pigs; D006624:Hippocampus / Q000196:embryology / Q000473:pathology / Q000503:physiopathology; D018782:Maze Learning / Q000187:drug effects; D008568:Memory / Q000187:drug effects; D009473:Neuronal Plasticity / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D009435:Synaptic Transmission / Q000187:drug effects; D013723:Teratogens / Q000633:toxicity*; D014810:Vitamin E / Q000009:adverse effects / Q000494:pharmacology*; D014815:Vitamins / Q000009:adverse effects / Q000494:pharmacology*
chemical_list: D013723:Teratogens; D014815:Vitamins; D014810:Vitamin E; D000431:Ethanol; D001205:Ascorbic Acid