title: Helicobacter pylori isolated from a patient with Ménétrier's disease increases hepatocyte growth factor mRNA expression in gastric fibroblasts: comparison with Helicobacter pylori isolated from other gastric diseases.
abstract: Ménétrier's disease has been reported to be associated with Helicobacter pylori infection. The aim of this study was to investigate the genetic characteristics of various virulence factors and cytokine expression profiles in Helicobacter pylori isolated from patients with Ménétrier's disease. The genotyping of virulence factors was accomplished by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Induction of various cytokines in MKN45 cells or gastric fibroblasts by Helicobacter pylori stimulus was measured by real-time reverse transcription-PCR. We found that the Helicobacter pylori strain isolated from a patient with Ménétrier's disease was different from other strains in the MseI-RFLP pattern of the ureC gene. Helicobacter pylori isolated from a patient with Ménétrier's disease showed the highest hepatocyte growth factor (HGF) and TNF-alpha mRNA expressions from gastric fibroblasts, and the highest TNF-alpha expression from MKN45 cells. The results in this study suggest that the difference in cytokine production, depending on the difference in bacteria components, plays an important role in the development of Ménétrier's disease.
authors.affiliation: Department of Gastroenterology, Matsushita Memorial Hospital, 5-55 Sotojima-cho, Moriguchi City, Osaka 570-8540, Japan. ishikawa.takeshi002@jp.panasonic.com
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Ando
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Obayashi
authors.forename: Nami
authors.initials: N
authors.lastname: Nakabe
authors.forename: Mika
authors.initials: M
authors.lastname: Okita
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Isozaki
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Nagao
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Oyamada
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Haruki
authors.initials: H
authors.lastname: Kato
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kokura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Naito
authors.forename: Norimasa
authors.initials: N
authors.lastname: Yoshida
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Yoshikawa
mesh_terms: D016207:Cytokines / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D005758:Gastritis, Hypertrophic / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D005838:Genotype; D016481:Helicobacter Infections / Q000150:complications* / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013272:Stomach Diseases / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014774:Virulence
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D017228:Hepatocyte Growth Factor