Collection of oocytes through transvaginal ovum pick-up for in vitro embryo production in Nanyang Yellow cattle.
The objectives of this study were to use ultrasound-guided transvaginal follicular aspiration to develop a technique for preservation of Nanyang Yellow cattle germ plasm. In Nanyang Yellow heifers, aspiration twice per week yielded a higher mean numbers of aspirated follicles, recovered oocytes and viable oocytes per session than once-weekly aspiration. Intriguingly, twice-weekly aspiration from Holstein heifers yielded a higher mean numbers of aspirated follicles, recovered oocytes and viable oocytes per session than in Nanyang Yellow heifers. Moreover, the oocyte recovery rate was significantly higher in Holstein heifers than in Nanyang Yellow heifers. Importantly, similar rates of embryos undergoing cleavage division were observed in Nanyang Yellow and Holstein, as was the number of viable day 8 blastocysts. These observations demonstrate that twice-weekly aspiration harvested the similar proportions of viable oocytes from Nanyang Yellow and Holstein follicles, and that twice-weekly aspiration yields a higher number of viable oocytes than once-weekly aspiration in Nanyang Yellow heifers. The results also suggest that the combination of ultrasound-guided transvaginal follicular aspiration and in vitro production is an effective and practical strategy for establishment of a germ plasm preservation programme for Nanyang Yellow cattle.
Li
F
F
Development Biology Research Center, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, No. 227 Chongqi South Road, Shanghai, China.
Chen
X
X
Pi
W
W
Liu
C
C
Shi
Z
Z
D000818:Animals; D002417:Cattle / Q000196:embryology / Q000502:physiology*; D003247:Conservation of Natural Resources; D005260:Female; D018587:Oocyte Donation / Q000379:methods / Q000662:veterinary*; D009865:Oocytes / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology* / Q000000981:diagnostic imaging / Q000502:physiology*; D010063:Ovum / Q000166:cytology / Q000502:physiology; D011247:Pregnancy; D013396:Suction; D013997:Time Factors; D014463:Ultrasonography; D014621:Vagina