Screening of the osmotic pressure-inducible promoter regions from the whole genome of Escherichia coli by using a novel cloning method.
Promoters belong to the most important regulatory domains in genomic sequences. A novel cloning method is described to produce various DNA fragments with the average size about 100 bp, which differ by 1 bp, for screening of the osmotic pressure-inducible promoters from the genome of Escherichia coli. An osmotic pressure-inducible promoter sequence was found. A mutation in this promoter sequence, introduced by site-specific mutagenesis, abolished its activity under the high osmotic pressure conditions. The method can thus be used for finding particular promoter sequences in the E. coli genome.
Wang
Zhijun
Z
CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, P.R. China. zjwang@shmu.edu.cn
Jin
Li
L
Wegrzyn
Grzegorz
G
Wegrzyn
Alicja
A
D003001:Cloning, Molecular / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D016680:Genome, Bacterial; D008957:Models, Genetic; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009997:Osmotic Pressure; D011401:Promoter Regions, Genetic / Q000235:genetics*
D004269:DNA, Bacterial