Ydj1 but not Sis1 stabilizes Hsp70 protein under prolonged stress in vitro.
Yeast cytosol has two important co-chaperons; Ydj1 and Sis1. Genetic experiments showed that Ydj1 is not essential for viability; however, cells lacking it grow very poorly at 30 degrees C or unable to grow at extreme temperatures. On the other hand, Sis1 is an essential protein and apparently plays a functional role at assembly or disassembly of protein complexes. Stability experiments revealed that only Ydj1-protected Hsp70 proteins can hydrolyze ATP under prolonged stress.
Tutar
Lütfi
L
Chemistry and Biochemistry Department, Faculty of Science, Cumhuriyet University, Sivas 58140, Turkey. ytutar@cumhuriyet.edu.tr
Tutar
Yusuf
Y
D000251:Adenosine Triphosphatases / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004795:Enzyme Stability; D050956:HSP40 Heat-Shock Proteins / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008156:Luciferases / Q000378:metabolism; D018384:Oxidative Stress; D011485:Protein Binding; D017510:Protein Folding; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013696:Temperature
D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C070320:SIS1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C070319:YDJ1 protein, S cerevisiae; D008156:Luciferases; D000251:Adenosine Triphosphatases