[Nitrification performance and microbial community analysis in carbon membrane-aerated biofilm reactor].
A carbon membrane-aerated biofilm reactor was developed to treat nitrogenous inorganic wastewater. Influent NH; -N concentrations and HRT were changed to investigate nitrification performance of reactor,oxygen utilization and NH4+ -N's removal loading. Biofilm's surface characteristics and dominant bacteria of nitrifier were analyzed. The results show that under the conditions of intra-membrane pressure of 0.017 MPa, influent NH4+ -N of 50 mg/L and HRT of 8 h NH4+ -N removal efficiency reaches 96% and effluent average nitrite is 17 mg/L, which benefits short-cut nitrification to a certain extent. The bacteria within biofilm consume all oxygen supplied through carbon membrane. The maximum specific removal rate of NH4+ -N is 9.7 g/(m2 x d), which is limited by the amount of bacteria grown onto carbon membrane's surface. Fluorescent in situ hybridization analysis indicates that within the biofilm Nitrosomonas and Nitrosospira are main ammonia-oxidizing bacteria and occupy about 19% and 21% of the total bacteria number, respectively. The Nitrobacter are not observed and Nitrospira are dominant nitrite-oxidizing bacteria, the fraction of which is 20% of total bacteria.
Liu
Hui-jun
HJ
School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China. lhj1130@sina.com
Yang
Feng-lin
FL
Zhang
Han-min
HM
Hu
Shao-wei
SW
Sun
Cui
C
D000641:Ammonia / Q000737:chemistry; D018441:Biofilms; D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000737:chemistry*; D009573:Nitrites / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry*; D043369:Nitrosomonadaceae / Q000145:classification / Q000378:metabolism / Q000502:physiology*; D009606:Nitrosomonas / Q000378:metabolism / Q000502:physiology; D011157:Population Dynamics; D014865:Waste Disposal, Fluid / Q000379:methods
D009573:Nitrites; D002244:Carbon; D000641:Ammonia; D009584:Nitrogen