Characterization of teleost phagocyte NADPH oxidase: molecular cloning and expression analysis of carp (Cyprinus carpio) phagocyte NADPH oxidase.
We previously demonstrated that some fish have unique response in the form of reactive oxygen species (ROS) production. In the present study, we cloned and sequenced the full-length cDNAs for carp (Cyprinus carpio) phagocyte NADPH oxidase components: gp91phox, p22phox, p47phox, p67phox and p40phox. These amino acid sequences were compared with other teleost and mammalian homologues, to elucidate the features of ROS production of fish neutrophils. The phylogeny analysis clearly demonstrates that the radiation of phagocyte oxidase components took place in the common ancestor of teleosts and mammals. Thereafter, the overall structure and expression pattern of phagocyte oxidase have been highly conserved in two different strains. However the amino acid identity of p67phox and p47phox was relatively lower than the amino acid identities of other components. Moreover, a synteny analysis supports the hypothesis that there was strong selective pressure in the p67phox and p47phox genes. Thus, it is likely that the higher divergence of p67phox and p47phox are responsible for the difference of ROS responses between different species of teleosts.
Mayumi
Masayuki
M
Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252-8510, Japan.
Takeda
Yoko
Y
Hoshiko
Masataka
M
Serada
Ken
K
Murata
Michihiro
M
Moritomo
Tadaaki
T
Takizawa
Fumio
F
Kobayashi
Isao
I
Araki
Kyosuke
K
Nakanishi
Teruyuki
T
Sumimoto
Hideki
H
D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D007962:Leukocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism*; D010586:Phagocytes / Q000201:enzymology*; D010802:Phylogeny
D019255:NADPH Oxidases