DNA polymerase I is not required for replication of linear chromosomes in streptomyces.
Both polA (encoding DNA polymerase I; Pol I) and a paralog were deleted from Streptomyces strains. Despite the UV sensitivity and slow growth caused by the DeltapolA mutation, the double mutant was viable. Thus, in contrast to a previous postulate, Pol I and its paralog are not essential for replication of Streptomyces chromosomes.
Huang
Tzu-Wen
TW
Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan.
Chen
Carton W
CW
D002876:Chromosomes, Bacterial / Q000378:metabolism*; D004256:DNA Polymerase I / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000096:biosynthesis*; D017353:Gene Deletion; D050296:Microbial Viability; D016254:Mutagenesis, Insertional; D013302:Streptomyces / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D004256:DNA Polymerase I