title: Determination of long-acting release octreotide, an octapeptide analogue of somatostation, in human plasma by liquid chromatography/tandem mass spectrometry.
abstract: A sensitive and selective method for the determination of long-acting released octreotide in human plasma has been developed based on liquid chromatography/tandem mass spectrometry (LC/MS/MS). Octreotide and the internal standard, triptorelin, were precipitated from the matrix, washed with dichloromethane and subsequently separated by reversed-phase high-performance liquid chromatography (HPLC) employing a 1% formic acid/methanol gradient system. Detection was by electrospray ionization mass spectrometry in the positive ion mode using multiple-reaction monitoring. The assay was linear in the concentration range 0.0500-50.0 ng/mL with intra- and inter-day precision (as relative standard deviation) of <2.95% and <8.37%, respectively. The limit of detection was 0.0200 ng/mL. The method was applied to a pharmacokinetic study of long-acting released octreotide in healthy volunteers given an intramuscular injection containing 20 mg octreotide.
authors.affiliation: Research Center for Drug Metabolism, College of Life Science, Jilin University, Changchun, P.R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Jiang
authors.forename: Jiang
authors.initials: J
authors.lastname: Wang
authors.forename: Yingwu
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Du
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Zhang
authors.forename: J Paul
authors.initials: JP
authors.lastname: Fawcett
authors.forename: Jingkai
authors.initials: J
authors.lastname: Gu
mesh_terms: D018931:Antineoplastic Agents, Hormonal / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D003692:Delayed-Action Preparations; D006801:Humans; D007273:Injections, Intramuscular; D008297:Male; D015282:Octreotide / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D013004:Somatostatin / Q000031:analogs & derivatives*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D018931:Antineoplastic Agents, Hormonal; D003692:Delayed-Action Preparations; D013004:Somatostatin; D015282:Octreotide