Catalytic features and eradication ability of antibody light-chain UA15-L against Helicobacter pylori.
We have successfully developed a catalytic antibody capable of degrading the active site of the urease of Helicobacter pylori and eradicating the bacterial infection in a mouse stomach. This monoclonal antibody UA15 was generated using a designed recombinant protein UreB, which contained the crucial region of the H. pylori urease beta-subunit active site, for immunization. The light chain of this antibody (UA15-L) by itself showed a proteolytic activity to substantially degrade both UreB and the intact urease. Oral administration of UA15-L also significantly reduced the number of H. pylori in a mouse stomach. This is the first example of a monoclonal catalytic antibody capable of functioning in vivo, and such an antibody may have a therapeutic utility in the future.
Hifumi
Emi
E
Research Center for Applied Medical Engineering, Oita University, Dan-noharu 700, Oita-shi, Oita 870-1192, Japan.
Morihara
Fumiko
F
Hatiuchi
Kenji
K
Okuda
Takuro
T
Nishizono
Akira
A
Uda
Taizo
T
D000595:Amino Acid Sequence; D000818:Animals; D017151:Antibodies, Catalytic / Q000235:genetics / Q000627:therapeutic use*; D001706:Biopsy; D016481:Helicobacter Infections / Q000276:immunology* / Q000473:pathology; D016480:Helicobacter pylori / Q000201:enzymology / Q000276:immunology*; D007074:Immunoglobulin G / Q000235:genetics / Q000627:therapeutic use; D007147:Immunoglobulin Light Chains / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use; D007145:Immunoglobulin kappa-Chains / Q000627:therapeutic use; D051379:Mice; D008958:Models, Molecular; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics; D013272:Stomach Diseases / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D014510:Urease / Q000737:chemistry / Q000235:genetics
D017151:Antibodies, Catalytic; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D012333:RNA, Messenger; D014510:Urease