Molecular imaging of endogenous mRNA expression in a mouse tumor model by adenovirus harboring trans-splicing ribozyme.
We previously showed that a trans-splicing ribozyme reprograms tumor-related genes at the mRNA level, resulting in the expression of therapeutic genes and that this approach can be efficiently employed to target specific molecules. Here, we show that trans-splicing ribozyme technology can be applied in molecular imaging of specific RNA expression in living animals. We exemplify this concept successfully by imaging mouse cytoskeleton-associated protein 2 (mCKAP2) expression in intrahepatic tumor nodules using systemically delivered adenovirus harboring mCKAP2-specific trans-splicing ribozyme.
Hong
Seung-Hee
SH
Research Institute, National Cancer Center, Goyang 410-769, Republic of Korea.
Jeong
Jin-Sook
JS
Lee
Yoon-Jong
YJ
Jung
Haeng-Im
HI
Kim
Kyung Tae
KT
Kim
Yun-Hee
YH
Lee
Yeon-Su
YS
Lee
Seong-Wook
SW
Bae
Chang-Dae
CD
Park
Joobae
J
Kim
In-Hoo
IH
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D003952:Diagnostic Imaging; D004195:Disease Models, Animal; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics*; D016337:RNA, Catalytic / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D020040:Trans-Splicing / Q000235:genetics*
C494575:CKAP2 protein, mouse; D003598:Cytoskeletal Proteins; D016337:RNA, Catalytic; D012333:RNA, Messenger