Effect of the heme oxygenase inducer hemin on blood haemostasis measured by high-frequency ultrasound.
1. Heme compounds, like hemin, a heme oxygenase-1 inducer, are used in the treatment of acute porphyria treatment. The side-effects of hemin on haemostasis have been reported. To address those effects, in the present study we used a sensitive, high-frequency ultrasound technique to record acoustic velocity and to investigate whole blood clotting in Wistar rats treated chronically with hemin (50 mg/kg per day). 2. The hemin-induced disturbances in haemostasis measured were comparable to the heparin reference treatment, with a significant decrease in clotting velocity in both groups compared with controls (e.g. the time to clot was 40 +/- 5, 53 +/- 13 and 10 +/- 2 min, respectively; P < 0.05). Precautions must be taken when using high doses of hemin or in the treatment of bleeding diseases. 3. Further investigations are required to explore the effects of hemin in thrombosis models, because it could be a promising 'old drug' for the treatment of venous thrombosis in patients.
Rochefort
Gaël Y
GY
LAB PART EA3852 IFR135, François Rabelais University, Faculty of Medicine, Tours, France.
Libgot
Rachel
R
Desbuards
Nicolas
N
Schlecht
Deborah
D
Halimi
Jean-Michel
JM
Ossant
Frederic
F
Eder
Veronique
V
Antier
Daniel
D
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D006418:Heme / Q000378:metabolism; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D006427:Hemin / Q000494:pharmacology*; D006487:Hemostasis / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014465:Ultrasonics; D020246:Venous Thrombosis
D000925:Anticoagulants; D006418:Heme; D006427:Hemin; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat