Biosensor for total cholesterol estimation using N-(2-aminoethyl)-3-aminopropyltrimethoxysilane self-assembled monolayer.
Cholesterol oxidase (ChOx), cholesterol esterase (ChEt), and horseradish peroxidase (HRP) have been co-immobilized covalently on a self-assembled monolayer (SAM) of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (AEAPTS) deposited on an indium-tin-oxide (ITO) glass surface. These enzyme-modified (ChOx-ChEt-HRP/AEAPTS/ITO) biosensing electrodes have been used to estimate cholesteryl oleate from 10 to 500 mg dL(-1). The sensitivity, Km value, and shelf-life of these ChEt-ChOx-HRP/AEAPTS/ITO biosensing electrodes have been found to be 124 nA mg(-1) dL, 95.098 mg dL(-1) (1.46 mmol L(-1)), and ten weeks, respectively. The ChEt-ChOx-HRP/AEAPTS/ITO bio-electrodes have been used to estimate total cholesterol in serum samples.
Arya
Sunil K
SK
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr K.S. Krishnan Marg, New Delhi, 110012, India.
Datta
Monika
M
Singh
S P
SP
Malhotra
Bansi D
BD
D015374:Biosensing Techniques / Q000295:instrumentation; D002784:Cholesterol / Q000097:blood*; D004800:Enzymes, Immobilized / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure; D011437:Propylamines / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*; D012826:Silicone Elastomers; D013499:Surface Properties
D004800:Enzymes, Immobilized; D011437:Propylamines; D012821:Silanes; D012826:Silicone Elastomers; C006194:aminoethyl-aminopropyl-trimethoxysilane; D002784:Cholesterol