title: In situ proteolysis for protein crystallization and structure determination.
abstract: We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
authors.affiliation: Structural Genomics Consortium, University of Toronto, 100 College Street, Toronto, Ontario M5G 1L5, Canada.
authors.forename: Aiping
authors.initials: A
authors.lastname: Dong
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Xu
authors.forename: Aled M
authors.initials: AM
authors.lastname: Edwards
authors.forename: Changsoo
authors.initials: C
authors.lastname: Chang
authors.forename: Maksymilian
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authors.lastname: Chruszcz
authors.forename: Marianne
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authors.lastname: Cuff
authors.forename: Marcin
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authors.lastname: Cymborowski
authors.forename: Rosa
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authors.lastname: Di Leo
authors.forename: Olga
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authors.lastname: Egorova
authors.forename: Elena
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authors.lastname: Evdokimova
authors.forename: Ekaterina
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authors.lastname: Filippova
authors.forename: Jun
authors.initials: J
authors.lastname: Gu
authors.forename: Jennifer
authors.initials: J
authors.lastname: Guthrie
authors.forename: Alexandr
authors.initials: A
authors.lastname: Ignatchenko
authors.forename: Andrzej
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authors.lastname: Joachimiak
authors.forename: Natalie
authors.initials: N
authors.lastname: Klostermann
authors.forename: Youngchang
authors.initials: Y
authors.lastname: Kim
authors.forename: Yuri
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authors.lastname: Korniyenko
authors.forename: Wladek
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authors.lastname: Minor
authors.forename: Qiuni
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authors.lastname: Que
authors.forename: Alexei
authors.initials: A
authors.lastname: Savchenko
authors.forename: Tatiana
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authors.lastname: Skarina
authors.forename: Kemin
authors.initials: K
authors.lastname: Tan
authors.forename: Alexander
authors.initials: A
authors.lastname: Yakunin
authors.forename: Adelinda
authors.initials: A
authors.lastname: Yee
authors.forename: Veronica
authors.initials: V
authors.lastname: Yim
authors.forename: Rongguang
authors.initials: R
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Zheng
authors.forename: Masato
authors.initials: M
authors.lastname: Akutsu
authors.forename: Cheryl
authors.initials: C
authors.lastname: Arrowsmith
authors.forename: George V
authors.initials: GV
authors.lastname: Avvakumov
authors.forename: Alexey
authors.initials: A
authors.lastname: Bochkarev
authors.forename: Lars-Göran
authors.initials: LG
authors.lastname: Dahlgren
authors.forename: Sirano
authors.initials: S
authors.lastname: Dhe-Paganon
authors.forename: Slav
authors.initials: S
authors.lastname: Dimov
authors.forename: Ludmila
authors.initials: L
authors.lastname: Dombrovski
authors.forename: Patrick
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authors.lastname: Finerty
authors.forename: Susanne
authors.initials: S
authors.lastname: Flodin
authors.forename: Alex
authors.initials: A
authors.lastname: Flores
authors.forename: Susanne
authors.initials: S
authors.lastname: Gräslund
authors.forename: Martin
authors.initials: M
authors.lastname: Hammerström
authors.forename: Maria Dolores
authors.initials: MD
authors.lastname: Herman
authors.forename: Bum-Soo
authors.initials: BS
authors.lastname: Hong
authors.forename: Raymond
authors.initials: R
authors.lastname: Hui
authors.forename: Ida
authors.initials: I
authors.lastname: Johansson
authors.forename: Yongson
authors.initials: Y
authors.lastname: Liu
authors.forename: Martina
authors.initials: M
authors.lastname: Nilsson
authors.forename: Lyudmila
authors.initials: L
authors.lastname: Nedyalkova
authors.forename: Pär
authors.initials: P
authors.lastname: Nordlund
authors.forename: Tomas
authors.initials: T
authors.lastname: Nyman
authors.forename: Jinrong
authors.initials: J
authors.lastname: Min
authors.forename: Hui
authors.initials: H
authors.lastname: Ouyang
authors.forename: Hee-won
authors.initials: HW
authors.lastname: Park
authors.forename: Chao
authors.initials: C
authors.lastname: Qi
authors.forename: Wael
authors.initials: W
authors.lastname: Rabeh
authors.forename: Limin
authors.initials: L
authors.lastname: Shen
authors.forename: Yang
authors.initials: Y
authors.lastname: Shen
authors.forename: Deepthi
authors.initials: D
authors.lastname: Sukumard
authors.forename: Wolfram
authors.initials: W
authors.lastname: Tempel
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Tong
authors.forename: Lionel
authors.initials: L
authors.lastname: Tresagues
authors.forename: Masoud
authors.initials: M
authors.lastname: Vedadi
authors.forename: John R
authors.initials: JR
authors.lastname: Walker
authors.forename: Johan
authors.initials: J
authors.lastname: Weigelt
authors.forename: Martin
authors.initials: M
authors.lastname: Welin
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
authors.forename: Ting
authors.initials: T
authors.lastname: Xiao
authors.forename: Hong
authors.initials: H
authors.lastname: Zeng
authors.forename: Haizhong
authors.initials: H
authors.lastname: Zhu
mesh_terms: D003460:Crystallization / Q000379:methods*; D003461:Crystallography / Q000379:methods*; D010447:Peptide Hydrolases / Q000737:chemistry*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure*
chemical_list: D011506:Proteins; D010447:Peptide Hydrolases