The role of Bach2 in nucleic acid-triggered antiviral innate immune responses.
Bach2, a bZIP transcription factor originally identified as interacting with the small Maf family of bZIP proteins, has been shown to play important roles in oxidative stress-mediated cell death. Here, we examine the role of Bach2 in cell death during double-stranded (ds)RNA- and dsDNA-triggered antiviral innate immune responses. Bach2 expression was induced in HeLa cells upon dsRNA/dsDNA treatment and the suppression of Bach2 expression by siRNA treatment alleviated cell death triggered by dsRNA and dsDNA. Unexpectedly, DNA microarray analysis revealed that siRNA-mediated suppression of Bach2 resulted in the attenuated activation of genes involved in the antiviral innate immune response after dsRNA treatment. Our study thus demonstrates a novel role for Bach2 as a key regulator of nucleic acid-triggered antiviral responses in human cells.
Hong
Sun Woo
SW
Department of Chemistry and BK School of Molecular Science, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
Kim
Soyoun
S
Lee
Dong-Ki
DK
D017209:Apoptosis / Q000235:genetics; D050976:Basic-Leucine Zipper Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D004279:DNA, Viral / Q000276:immunology* / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011070:Poly I-C / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000494:pharmacology; D012367:RNA, Viral / Q000276:immunology* / Q000494:pharmacology; D014780:Viruses / Q000276:immunology*
C491123:BACH2 protein, human; D050976:Basic-Leucine Zipper Transcription Factors; D004279:DNA, Viral; D034741:RNA, Small Interfering; D012367:RNA, Viral; D011070:Poly I-C