[Establishment of human retinoblastoma model in human immune reconstruction non-obese diabetic-severe combine immunodeficient mice].
OBJECTIVE
To develop a novel immunotherapy model of retinoblastoma (RB) in human PBL non-obese diabetic-severe combined immunodeficient (NOD-SCID) mice, and to investigate its biological features.


METHODS
Twenty NOD-SCID mice were randomly divided into 4 groups: group A, subcutaneous injection of phosphate buffered saline; group B, intraperitoneal injection of human peripheral blood lymphocyte (hu-PBL) for immune reconstruction; group C, subcutaneous injection of SO-RB50 cells and group D, intraperitoneal injection of hu-PBL with subcutaneous injection of SO-RB50 cells. The physical status of NOD-SCID mice and the tumor growth were observed. Human IgG in mouse serum was assayed by ELISA. Human T lymphocytes in murine spleen and peripheral blood were evaluated by flow cytometry. Histopathological examination and immunohistochemical studies were also performed.


RESULTS
In groups C and D, the latent period of tumor growth was 12 - 19 days, and the taken rates of RB were 100%. Histological and immunohistochemical results showed that the tumor cells of the two groups retained the characteristics of human low differentiation RB. More interesting, some human lymphocytes stained by human LCA presented in transplanted tumors and murine spleens. Human IgG and T lymphocytes were detected in humanized groups (groups B and D).


CONCLUSIONS
The RB model in the human PBL NOD-SCID mice has been successfully established, which simulates the biological behavior of human spontaneous RB. This model may be useful for the studying of immune regression and immunotherapy of human RB.
Zhong
Xiu-feng
XF
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, the Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510060, China.
Ge
Jian
J
Peng
Fu-hua
FH
Zhang
Bo
B
Lin
Jian-xian
JX
Zhang
Wen-xin
WX
Li
Yong-ping
YP
D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D012175:Retinoblastoma; D016511:Severe Combined Immunodeficiency