Differentiation and Ig-allele switch in cell line WEHI-231.
Because of its susceptibility to apoptosis upon Ag receptor cross-linking and lack of IgD expression, cells of the mouse cell line WEHI-231 have been classified as immature B cells. In this study we show that early freezings of the WEHI-231 line express IgD but not CD93, which classifies the cells as more similar to mature B cells. Another, later line obviously has differentiated in culture and has all the hallmarks of activated B cells. But despite activation-induced cytidine deaminase expression, there is no switch in isotype; instead we found switching from one mu allele to the other. As a consequence of these findings, we now view the apoptosis studies in the WEHI-231 line to reflect properties of mature and activated B lymphocytes, respectively.
Spillmann
Freia J X
FJ
Department of Microbiology and Immunology, University of California, San Francisco, CA 94143-0414, USA.
Beck-Engeser
Gabriele
G
Wabl
Matthias
M
D000483:Alleles; D000818:Animals; D000941:Antigens / Q000276:immunology; D000943:Antigens, Differentiation / Q000276:immunology / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics / Q000276:immunology*; D001402:B-Lymphocytes / Q000201:enzymology / Q000276:immunology*; D002460:Cell Line; D002462:Cell Membrane / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D003564:Cytidine Deaminase / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D004789:Enzyme Activation / Q000235:genetics / Q000276:immunology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000276:immunology; D018858:Germinal Center / Q000201:enzymology / Q000276:immunology; D006801:Humans; D007072:Immunoglobulin D / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D020522:Lymphoma, Mantle-Cell / Q000201:enzymology / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011947:Receptors, Antigen, B-Cell / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D027041:Somatic Hypermutation, Immunoglobulin / Q000235:genetics / Q000276:immunology*; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
D000941:Antigens; D000943:Antigens, Differentiation; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D003564:Cytidine Deaminase