Conjugation of bioactive groups to poly(lactic acid) and poly[(lactic acid)-co-(glycolic acid)] films.
A novel PLA-based polymer containing reactive pendent ketone or hydroxyl groups was synthesized by the copolymerization of L-lactide with epsilon-caprolactone-based monomers. The polymer was activated with NPC, resulting in an amine-reactive polymer which was then cast into thin polymeric films, either alone or as part of a blend with PLGA, before immersion into a solution of the cell adhesion peptide GRGDS in PBS buffer allowed for conjugation of GRGDS to the film surfaces. Subsequent 3T3 fibroblast cell adhesion studies demonstrated an increase in cellular adhesion and spreading over films cast from unmodified PLGA. Hence the new polymer can be used to obtain covalent linkage of amine-containing molecules to polymer surfaces.
Prime
Emma L
EL
Polymer Science Group, Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, VIC 3010, Australia.
Cooper-White
Justin J
JJ
Qiao
Greg G
GG
D016475:3T3 Cells; D000588:Amines; D000595:Amino Acid Sequence; D000818:Animals; D000873:Anthracenes; D002448:Cell Adhesion; D005347:Fibroblasts / Q000166:cytology; D005453:Fluorescence; D005561:Formates / Q000737:chemistry; D019344:Lactic Acid / Q000138:chemical synthesis / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011091:Polyesters; D011100:Polyglycolic Acid / Q000138:chemical synthesis / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011522:Protons
D000588:Amines; D000873:Anthracenes; D005561:Formates; D010455:Peptides; D011091:Polyesters; D011108:Polymers; D011522:Protons; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); C034020:anthracene