title: Novel expression of kallikreins, kallikrein-related peptidases and kinin receptors in human pleural mesothelioma.
abstract: Malignant mesothelioma is an aggressive cancer of the pleura that is causally related to exposure to asbestos fibres. The kallikrein serine proteases [tissue (hK1) and plasma (hKB1) kallikreins, and kallikrein-related peptidases (KRP/hK2-15)] and the mitogenic kinin peptides may have a role in tumourigenesis. However, it is not known whether hK1, hKB1, KRP/hK proteins or kinin receptors are expressed in pleural mesotheliomas. The expression of hK1, hKB1, KRP/hK2, 5, 6, 7, 8 and 9, and kinin B(1) and B(2) receptors was assessed in archived selected normal tissue and mesothelioma tumour sections by immunoperoxidase and immunofluorescence labelling. hK1, hKB1 and kinin B(1) and B(2) receptors were expressed in malignant cells of the epithelioid and sarcomatoid components of biphasic mesothelioma tumour cells. The percentage of cells with cytoplasmic and nuclear labelling and the intensity of labelling were similar for hK1, hKB1 and the kinin receptors. KRP/hK2, 6, 8 and 9 were also expressed in the cytoplasm and nuclei of mesothelioma cells, whereas KRP/hK5 and hK7 showed predominantly cytoplasmic localisation. This is a first report, but further studies are required to determine whether these proteins have a functional role in the pathogenesis of mesothelioma and/or may be potential biomarkers for pleural mesothelioma.
authors.affiliation: Lung Institute of Western Australia, Centre for Asthma, Allergy and Respiratory Research, The University of Western Australia, Nedlands WA, Australia.
authors.forename: Jessica
authors.initials: J
authors.lastname: Chee
authors.forename: Jasmine
authors.initials: J
authors.lastname: Singh
authors.forename: Anupam
authors.initials: A
authors.lastname: Naran
authors.forename: Neil L
authors.initials: NL
authors.lastname: Misso
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Thompson
authors.forename: Kanti D
authors.initials: KD
authors.lastname: Bhoola
mesh_terms: D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007610:Kallikreins / Q000378:metabolism*; D007705:Kinins / Q000378:metabolism*; D008654:Mesothelioma / Q000201:enzymology / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D010997:Pleural Neoplasms / Q000201:enzymology / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D007705:Kinins; D011956:Receptors, Cell Surface; D010447:Peptide Hydrolases; D007610:Kallikreins