Genetic distribution of 295 Bacillus cereus group members based on adk-screening in combination with MLST (Multilocus Sequence Typing) used for validating a primer targeting a chromosomal locus in B. anthracis.
The genetic distribution of 295 Bacillus cereus group members has been investigated by using a modified Multilocus Sequence Typing method (MLST). By comparing the nucleic acid sequence of the adk gene fragment, isolates of B. cereus group members most related to B. anthracis may be easily identified. The genetic distribution, with focus on the B. anthracis close neighbours, was used to evaluate a new primer set for specific identification of B. anthracis. This primer set, BA5510-1/2, targeted the putative B. anthracis specific gene BA5510. Real-time PCR using BA5510-1/2 amplified the target fragment from all B. anthracis strains tested and only two (of 289) non-B. anthracis strains analysed. This is one of the most thoroughly validated chromosomal B. anthracis markers for real-time PCR identification, in which the screened collection contained several very closely related B. anthracis strains.
Olsen
Jaran S
JS
Forsvarets forskningsinstitutt FFI, Norwegian Defence Research Establishment, P. O. Box 25, N-2027 Kjeller, Norway. Jaran-Strand.Olsen@ffi.no
Skogan
Gunnar
G
Fykse
Else Marie
EM
Rawlinson
Elizabeth L
EL
Tomaso
Herbert
H
Granum
Per Einar
PE
Blatny
Janet M
JM
D000263:Adenylate Kinase / Q000235:genetics* / Q000378:metabolism; D001408:Bacillus anthracis / Q000145:classification* / Q000235:genetics; D001409:Bacillus cereus / Q000145:classification* / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D002876:Chromosomes, Bacterial / Q000235:genetics; D017931:DNA Primers; D004269:DNA, Bacterial; D005819:Genetic Markers / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D004269:DNA, Bacterial; D005819:Genetic Markers; D000263:Adenylate Kinase