title: Mouse mammary tumor virus-like gene sequences in breast cancer samples of Mexican women.
abstract: BACKGROUND
  Previous reports related the presence of mouse mammary tumor virus (MMTV)-like gene sequences to human breast carcinoma. The aim of this study was to determine whether MMTV-like env gene sequences are present in breast cancer samples of Mexican women and in breast and lung cancer cell lines.
  
  
  METHODS
  Using specific primers for MMTV, we tested 3 breast cancer cell lines, 4 non-small lung cancer cell lines and 119 breast cancer samples from Mexican women.
  
  
  RESULTS
  MMTV-like gene sequences were amplified in the lung cancer cell INER-51, but not in the MCF-7 cell line that has been used as a positive control in other reports and in 5 of 119 (4.2%) breast cancer biopsy tissues. Furthermore, the identity of sequences of PCR products from INER-51 and a breast cancer-positive sample are 98 and 99% when compared with the env region of MMTV (GenBank accession No. AY161347).
  
  
  CONCLUSIONS
  These results indicate that MMTV-like gene sequences are present in the Mexican population.
authors.affiliation: Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), Ciudad Universitaria, San Nicolás de los Garza, NL, México.
authors.forename: P
authors.initials: P
authors.lastname: Zapata-Benavides
authors.forename: S
authors.initials: S
authors.lastname: Saavedra-Alonso
authors.forename: D
authors.initials: D
authors.lastname: Zamora-Avila
authors.forename: C
authors.initials: C
authors.lastname: Vargas-Rodarte
authors.forename: R
authors.initials: R
authors.lastname: Barrera-Rodríguez
authors.forename: J
authors.initials: J
authors.lastname: Salinas-Silva
authors.forename: C
authors.initials: C
authors.lastname: Rodríguez-Padilla
authors.forename: R
authors.initials: R
authors.lastname: Tamez-Guerra
authors.forename: L
authors.initials: L
authors.lastname: Trejo-Avila
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology / Q000821:virology*; D002460:Cell Line; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D015752:Genes, env; D006801:Humans; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics* / Q000302:isolation & purification*; D008800:Mexico; D008969:Molecular Sequence Data
chemical_list: D017931:DNA Primers; D004273:DNA, Neoplasm