Effects of gamma irradiation on mechanical behavior and calcification of glutaraldehyde-fixed bovine pericardium.
OBJECTIVE
To evaluate the effect of gamma irradiation on glutaraldehyde-fixed bovine pericardium.


METHODS
Glutaraldehyde-fixed bovine pericardium was exposed to gamma radiation (doses from 0 to 10000 Gy). Six samples from each of nine groups were evaluated by optic microscopy, and shrinking and mechanical tests and the denaturation temperature was determined. Additionally, they were subcutaneously implanted in rats and after four months they were explanted and Ca2+ levels measured by atomic absorption spectroscopy.


RESULTS
The Ca2+ levels were (in microg/mg): control (0 Gy) - 194.45; 50 Gy - 154.64; 100 Gy - 169.37; 200 Gy - 163.64; 500 Gy - 199.89; 1000 Gy - 184.02; 2000 Gy - 198.95; 5000 Gy - 227.95; 10000 Gy - 362.62. Gamma irradiation caused a significant effect on the biomechanical properties of the tissue.


CONCLUSIONS
e-fixed bovine pericardium.
Baucia
José Augusto
JA
Instituto de Pesquisas Energéticas e Nucleares(IPEN) - Linked to the University of São Paulo, São Paulo, SP. jabaucia@hotmail.com
Leal Neto
Ricardo Mendes
RM
Rogero
José Roberto
JR
Nascimento
Nanci do
Nd
Reyes
Carmem Aparecida Cruz
CA
D000469:Alkaline Phosphatase / Q000032:analysis / Q000528:radiation effects*; D000818:Animals; D002114:Calcinosis / Q000517:prevention & control*; D002118:Calcium / Q000032:analysis; D002417:Cattle; D003094:Collagen; D004195:Disease Models, Animal; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005404:Fixatives / Q000494:pharmacology*; D005720:Gamma Rays / Q000627:therapeutic use*; D005976:Glutaral / Q000494:pharmacology*; D010496:Pericardium / Q000528:radiation effects*; D019736:Prostheses and Implants; D051381:Rats; D013314:Stress, Mechanical
D005404:Fixatives; D003094:Collagen; D000469:Alkaline Phosphatase; D002118:Calcium; D005976:Glutaral