title: Regenerative potential of embryonic renal multipotent progenitors in acute renal failure.
abstract: Bone marrow-and adult kidney-derived stem/progenitor cells hold promise in the development of therapies for renal failure. Here is reported the identification and characterization of renal multipotent progenitors in human embryonic kidneys that share CD24 and CD133 surface expression with adult renal progenitors and have the capacity for self-renewal and multilineage differentiation. It was found that these CD24+CD133+ cells constitute the early primordial nephron but progressively disappear during nephron development until they become selectively localized to the urinary pole of Bowman's capsule. When isolated and injected into SCID mice with acute renal failure from glycerol-induced rhabdomyolysis, these cells regenerated different portions of the nephron, reduced tissue necrosis and fibrosis, and significantly improved renal function. No tumorigenic potential was observed. It is concluded that CD24+CD133+ cells represent a subset of multipotent embryonic progenitors that persist in human kidneys from early stages of nephrogenesis. The ability of these cells to repair renal damage, together with their apparent lack of tumorigenicity, suggests their potential in the treatment of renal failure.
authors.affiliation: Excellence Center for Research, Transfer and High Education for the Development of DE NOVO THERAPIES, University of Florence, Florence, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Lazzeri
authors.forename: Clara
authors.initials: C
authors.lastname: Crescioli
authors.forename: Elisa
authors.initials: E
authors.lastname: Ronconi
authors.forename: Benedetta
authors.initials: B
authors.lastname: Mazzinghi
authors.forename: Costanza
authors.initials: C
authors.lastname: Sagrinati
authors.forename: Giuseppe Stefano
authors.initials: GS
authors.lastname: Netti
authors.forename: Maria Lucia
authors.initials: ML
authors.lastname: Angelotti
authors.forename: Eliana
authors.initials: E
authors.lastname: Parente
authors.forename: Lara
authors.initials: L
authors.lastname: Ballerini
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Cosmi
authors.forename: Laura
authors.initials: L
authors.lastname: Maggi
authors.forename: Loreto
authors.initials: L
authors.lastname: Gesualdo
authors.forename: Mario
authors.initials: M
authors.lastname: Rotondi
authors.forename: Francesco
authors.initials: F
authors.lastname: Annunziato
authors.forename: Enrico
authors.initials: E
authors.lastname: Maggi
authors.forename: Laura
authors.initials: L
authors.lastname: Lasagni
authors.forename: Mario
authors.initials: M
authors.lastname: Serio
authors.forename: Sergio
authors.initials: S
authors.lastname: Romagnani
authors.forename: Gabriella Barbara
authors.initials: GB
authors.lastname: Vannelli
authors.forename: Paola
authors.initials: P
authors.lastname: Romagnani
mesh_terms: D000071916:AC133 Antigen; D000208:Acute Disease; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D051927:CD24 Antigen / Q000096:biosynthesis; D004622:Embryo, Mammalian / Q000166:cytology*; D006023:Glycoproteins / Q000096:biosynthesis; D006801:Humans; D007684:Kidney Tubules / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D018613:Microscopy, Confocal; D009399:Nephrons / Q000473:pathology; D010455:Peptides; D012038:Regeneration; D051437:Renal Insufficiency / Q000378:metabolism / Q000473:pathology*; D012206:Rhabdomyolysis / Q000473:pathology / Q000628:therapy; D013234:Stem Cells / Q000166:cytology*
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D051927:CD24 Antigen; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse