Rapid characterization of polyalcohols by silylation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
A matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry method for rapidly enumerating hydroxyl groups in analytes is described, and applied to some common polyalcohols (erythritol, mannitol and xylitol). Polyalcohols were derivatized with trimethylsilylimidazole (TMSI) either separately or as mixtures, and were analyzed, without chromatographic separation or purification. The mass spectra revealed consecutive peaks that are separated by 72 m/z units as a consequence of displacement of one hydroxyl hydrogen atom by one TMS group. The number of observed peaks was used to confirm the number of hydroxyl groups in each analyte.
Adeuya
Anthony
A
U.S. Department of Agriculture, Agricultural Research Service-NCAUR, Bioproducts and Biocatalysis Research Unit, 1815 North University Street, Peoria, IL 61604, USA. anthony.adeuya@ars.usda.gov
Price
Neil
N
D004896:Erythritol / Q000737:chemistry; D008353:Mannitol / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013402:Sugar Alcohols / Q000737:chemistry*; D014297:Trimethylsilyl Compounds / Q000737:chemistry*; D014993:Xylitol / Q000737:chemistry
D013402:Sugar Alcohols; D014297:Trimethylsilyl Compounds; D008353:Mannitol; D004896:Erythritol; D014993:Xylitol