The diacylglycerol forming pathways differ among floral organs of Petunia hybrida.
The origin of diacylglycerol, a substrate for membrane lipid biosynthesis, is not fully understood. Here, we report that Petunia hybrida floral organs contain large amounts of diacylglycerol. Our data suggest that in stamens and pistils diacylglycerol is supplied both from phosphatidylcholine by non-specific phospholipase C activity and de novo via the Kennedy pathway and phosphatidic acid phosphatase, whereas in petals the two-step pathway catalyzed by phospholipase D and phosphatidic acid phosphatase predominates. Therefore, the pathways that supply diacylglycerol differ among floral reproductive organs, although large amounts of diacylglycerol are commonly accumulated in these organs.
Nakamura
Yuki
Y
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B-65 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
Ohta
Hiroyuki
H
D000123:Acetyltransferases / Q000378:metabolism; D004075:Diglycerides / Q000096:biosynthesis*; D005227:Fatty Acids / Q000378:metabolism; D035264:Flowers / Q000378:metabolism*; D032306:Petunia / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism
C011439:1,2-diacylglycerol; D004075:Diglycerides; D005227:Fatty Acids; D010743:Phospholipids; D000123:Acetyltransferases