[Analysis of sperm chromosome in patients with chromosome balanced translocation by fluorescence in-situ hybridization].
OBJECTIVE
To investigate the chromosome segregation patterns in patients with chromosome balanced translocation in spermatogenesis and to find out different proportions of abnormal sperm generated by different patterns.


METHODS
Triple color fluorescence in-situ hybridization (FISH) was used to determine the correlative chromosome constitution of spermatozoa from 4 patients with chromosome anomalies, Case 1 with 46, XY, t(9;11)(q22;q21), Case 2 with 46, XY, t(11;22) (q23;q11), Case 3 with 45, XY, t(13q;15q) and Case 4 with 45, XY, t(13q;14q). The numbers of spermatozoa generated by different chromosome segregation patterns were counted by FISH, and normal sperm from the males with normal chromosome was used as control.


RESULTS
The rates of abnormal sperm in the 4 cases were 50.86%, 58.33%, 13.00% and 22.82% respectively, evidently higher than their corresponding controls (0.85%, 1.63%, 1.60% and 1.37%).


CONCLUSIONS
To detect sperm chromosome in patients with chromosome balanced translocation by FISH can provide valuable information for predicting the risk of chromosome anomalies in the embryo preimplantation genetic diagnosis.
Zeng
Yong
Y
IVF Center of Shenzhen Zhongshan Urological Hospital, Shenzhen, Guangdong 518001, China. dearivf@sina.com
Hu
Xiao-Dong
XD
Lin
Qi
Q
Song
Cheng
C
D000328:Adult; D025063:Chromosome Disorders / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D008297:Male; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*; D014178:Translocation, Genetic