title: [Determination of three alkaloids in total alkaloids exfracts from Lotus plumule by RP-HPLC].
abstract: OBJECTIVE
  To develop a RP-HPLC method for determination of three alkaloids in total alkaloids exfracted from Lotus plumule.
  
  
  METHODS
  A Hypersil C18 column was used with the mobile phase of methanol-phosphate (73:27, pH 9.0) at the detection wavelength of 230 and 286 nm. The flow rate was 1 mL min(-1).
  
  
  RESULTS
  The linear range was 20-700 microg mL(-1), the recovery of three alkaloids were 97.9%, 98.4% and 98.1%. The RSD of intra-day and inter-day was 0. 24% -4. 83%. Different groups of samples were determined by this method. The result showed that the extraction method with methanol was prior to the extraction method with alcohol.
  
  
  CONCLUSIONS
  The method is simple, rapid and suitable for the determination of three alkaloids in total alkaloids exfracted from L. plumule.
authors.affiliation: Department of Pharmacology, Tongji Medical College, Huangzhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Li-bo
authors.initials: LB
authors.lastname: Zhao
authors.forename: You
authors.initials: Y
authors.lastname: Jin
authors.forename: Jia-ling
authors.initials: JL
authors.lastname: Wang
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D044182:Benzylisoquinolines / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D007546:Isoquinolines / Q000032:analysis*; D000070116:Lotus / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry
chemical_list: D000470:Alkaloids; D044182:Benzylisoquinolines; D004365:Drugs, Chinese Herbal; D007546:Isoquinolines; D010636:Phenols; C499779:isoliensinine; C057222:neferine; C080095:liensinine