title: Hair analysis to document a clinical case of TCDD over-exposure.
abstract: Dioxins and related compounds (furans) are persistent environmental contaminants that cause adverse biological effects. Their influence on humans is still unclear, except for accidental high-dose exposure. Chronic exposure to these compounds seems to be involved in cancer, endocrine disruption and neurobehavioral effects. For several years, a large concern about the potential health risks of dioxins is emerging in Europe and United States. The case of a 50-year-old man victim of an acute over-exposure to tetrachloro dibenzo-p-dioxin (TCDD or Seveso dioxin) is reported here with particular focus on hair investigations. The developed method involved the decontamination of the hair strand using picograde level methylene chloride, the homogenization of hair segments with scissors and their extraction in presence of 13C12-marked dioxin congeners under reflux of toluene using a Soxhlet, 8h at 130 degrees C. After reduction of the toluene fraction to 1 ml and addition of purification marker (37Cl4-2,3,7,8-TCDD), dioxins purification was achieved using three successive columns: silica, alumina/sodium sulfate and carbon/Celite columns. Finally, the toluene eluent was evaporated and the extract injected in the analytical system. After chromatographic separation, detection was achieved in single ion monitoring mode using a high resolution mass spectrometer operating in electron impact ionization mode (40 eV, minimal resolution of 10,000). The analysis of the first hair segment (0-6 cm) revealed the presence of 2,3,7,8-TCDD at 65 fg/mg when the distal one remained negative (LOQ=0.3 fg/mg). All other congeners (n=16) were in the range of those determined in the general population (0.62 and 0.89 fg/mg in the two hair segments, respectively). The extremely low dioxin levels generally found in hair specimens (low fg/mg range) lead us to analyze them using the very sensitive and specific gas chromatography-high resolution mass spectrometry apparatus. From the best of our knowledge, this is the first case of over-exposure to Seveso dioxin through hair analysis reported in the literature.
authors.affiliation: ChemTox, 3 rue Grüninger, F-67400 Illkirch Graffenstaden, France. vcirimele@labochemtox.com
authors.forename: V
authors.initials: V
authors.lastname: Cirimele
authors.forename: M
authors.initials: M
authors.lastname: Villain
authors.forename: G
authors.initials: G
authors.lastname: Salquèbre
authors.forename: C
authors.initials: C
authors.lastname: Staub
authors.forename: P
authors.initials: P
authors.lastname: Kintz
mesh_terms: D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000032:analysis* / Q000633:toxicity; D053593:Forensic Toxicology; D008401:Gas Chromatography-Mass Spectrometry; D006197:Hair / Q000737:chemistry*; D006801:Humans; D008297:Male; D008875:Middle Aged; D000072317:Polychlorinated Dibenzodioxins / Q000032:analysis* / Q000633:toxicity
chemical_list: D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins