Fur regulates expression of the Salmonella pathogenicity island 1 type III secretion system through HilD.
The invasion of intestinal epithelial cells by Salmonella enterica serovar Typhimurium is mediated by a type III secretion system (T3SS) encoded on Salmonella pathogenicity island 1 (SPI1). Expression of the SPI1 T3SS is tightly regulated by the combined action of HilC, HilD, and RtsA, three AraC family members that can independently activate hilA, which encodes the direct regulator of the SPI1 structural genes. Expression of hilC, hilD, and rtsA is controlled by a number of regulators that respond to a variety of environmental signals. In this work, we show that one such signal is iron mediated by Fur (ferric uptake regulator). Fur activates hilA transcription in a HilD-dependent manner. Fur regulation of HilD does not appear to be simply at the transcriptional or translational level but rather requires the presence of the HilD protein. Fur activation of SPI1 is not mediated through the Fur-regulated small RNAs RfrA and RfrB, which are the Salmonella ortholog and paralog of RyhB that control expression of sodB. Fur regulation of HilD is also not mediated through the known SPI1 repressor HilE or the CsrABC system. Although understanding the direct mechanism of Fur action on HilD requires further analysis, this work is an important step toward elucidating how various global regulatory systems control SPI1.
Ellermeier
Jeremy R
JR
Department of Microbiology, University of Illinois, B103 Chemical and Life Sciences Laboratory, 601 S. Goodwin Ave., Urbana, IL 61801, USA.
Slauch
James M
JM
D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000502:physiology*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D017930:Genes, Reporter; D008954:Models, Biological; D012097:Repressor Proteins / Q000502:physiology*; D012486:Salmonella typhimurium / Q000502:physiology*; D015534:Trans-Activators / Q000096:biosynthesis; D014157:Transcription Factors / Q000037:antagonists & inhibitors
D001426:Bacterial Proteins; C102236:HilA protein, Salmonella; C119660:HilD protein, Salmonella typhimurium; D012097:Repressor Proteins; C419942:Spi1 protein, Salmonella; D015534:Trans-Activators; D014157:Transcription Factors; C068477:ferric uptake regulating proteins, bacterial