title: PEGylated lipoplexes: preparation protocols affecting DNA condensation and cell transfection efficiency.
abstract: The inclusion of poly(ethylene glycol) monolaurate in liposomes formulated with dimyristoyl-sn-glycero-3-phosphocholine and certain cationic gemini surfactants improves their capability of condensing DNA into a psi phase and transfecting it into cells. Both the condensation, observed by circular dichroism, and the transfection efficiency are strongly effected by the protocol of inclusion of the polymer in the formulations. The highest transfection efficiency is observed in correspondence of the highest extent of DNA condensation.
authors.affiliation: Istituto di Metodologie Chimiche del CNR and Dipartimento di Chimica, Università degli Studi di Roma La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Bombelli
authors.forename: Francesca
authors.initials: F
authors.lastname: Faggioli
authors.forename: Paola
authors.initials: P
authors.lastname: Luciani
authors.forename: Giovanna
authors.initials: G
authors.lastname: Mancini
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Sacco
mesh_terms: D000818:Animals; D019556:COS Cells; D002412:Cations; D002522:Chlorocebus aethiops; D002942:Circular Dichroism; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008081:Liposomes; D011092:Polyethylene Glycols; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D014162:Transfection
chemical_list: D002412:Cations; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; C526823:poly(ethylene glycol) monolaurate; D049452:Green Fluorescent Proteins; D011092:Polyethylene Glycols; D004247:DNA; D004134:Dimyristoylphosphatidylcholine