title: Heat-resistant DNA tile arrays constructed by template-directed photoligation through 5-carboxyvinyl-2'-deoxyuridine.
abstract: Template-directed DNA photoligation has been applied to a method to construct heat-resistant two-dimensional (2D) DNA arrays that can work as scaffolds in bottom-up assembly of functional biomolecules and nano-electronic components. DNA double-crossover AB-staggered (DXAB) tiles were covalently connected by enzyme-free template-directed photoligation, which enables a specific ligation reaction in an extremely tight space and under buffer conditions where no enzymes work efficiently. DNA nanostructures created by self-assembly of the DXAB tiles before and after photoligation have been visualized by high-resolution, tapping mode atomic force microscopy in buffer. The improvement of the heat tolerance of 2D DNA arrays was confirmed by heating and visualizing the DNA nanostructures. The heat-resistant DNA arrays may expand the potential of DNA as functional materials in biotechnology and nanotechnology.
authors.affiliation: Department of Life Sciences and Institute of Physics, Graduate School of Arts and Sciences, The University of Tokyo, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Tagawa
authors.forename: Koh-ichiroh
authors.initials: K
authors.lastname: Shohda
authors.forename: Kenzo
authors.initials: K
authors.lastname: Fujimoto
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sugawara
authors.forename: Akira
authors.initials: A
authors.lastname: Suyama
mesh_terms: D020029:Base Pairing; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects* / Q000648:ultrastructure; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry; D006358:Hot Temperature; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology; D010777:Photochemistry; D014466:Ultraviolet Rays
chemical_list: C520201:5-carboxyvinyl-2'-deoxyuridine; D004247:DNA; D003857:Deoxyuridine