title: Biochemical composition and immunological comparison of select pecan [Carya illinoinensis (Wangenh.) K. Koch] cultivars.
abstract: On an edible portion basis, pecan moisture, protein, lipid, total soluble sugars, and ash contents ranged from 2.1% to 6.4%, 6.0% to 11.3%, 65.9% to 78.0%, 3.3% to 5.3%, and 1.2% to 1.8%, respectively. With the exception of a high tannin (2.7%) Texas seedling, pecan tannin content was in a narrow range (0.6-1.85%). Unsaturated fatty acids (>90%) dominated pecan lipid composition with oleic (52.52-74.09%) and linoleic (17.69-37.52%) acids as the predominant unsaturated fatty acids. Location significantly influenced pecan biochemical composition. Pecan lipid content was negatively correlated with protein (r = -0.663) and total sugar (r = -0.625). Among the samples tested using SDS-PAGE a common pattern, with minor differences, in subunit polypeptide profiles was revealed. Rabbit polyclonal antibody-based immunoblotting experiments (Western blot) also illustrated the similarity in polypeptide profiles with respect to immunoreactivity. All tested cultivars registered similar immunoreactivity when their protein extracts (each at 1 mg/mL) were assessed using inhibition ELISAs (mean +/- standard deviation = 0.89 +/- 0.20; n = 27) with the USDA "Desirable" cultivar as the reference standard (immunoreactivity designated as 1.0).
authors.affiliation: Department of Nutrition, Food, and Exercise Sciences, College of Human Sciences, Florida State University, Tallahassee, Florida 32306, USA.
authors.forename: Mahesh
authors.initials: M
authors.lastname: Venkatachalam
authors.forename: Harshal H
authors.initials: HH
authors.lastname: Kshirsagar
authors.forename: Navindra P
authors.initials: NP
authors.lastname: Seeram
authors.forename: David
authors.initials: D
authors.lastname: Heber
authors.forename: Tommy E
authors.initials: TE
authors.lastname: Thompson
authors.forename: Kenneth H
authors.initials: KH
authors.lastname: Roux
authors.forename: Shridhar K
authors.initials: SK
authors.lastname: Sathe
mesh_terms: D000906:Antibodies / Q000276:immunology; D002241:Carbohydrates / Q000032:analysis; D031325:Carya / Q000737:chemistry* / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D005504:Food Analysis; D009584:Nitrogen / Q000032:analysis; D009754:Nuts / Q000737:chemistry*; D010940:Plant Proteins / Q000032:analysis; D013045:Species Specificity; D013634:Tannins / Q000032:analysis
chemical_list: D000906:Antibodies; D002241:Carbohydrates; D005231:Fatty Acids, Unsaturated; D010940:Plant Proteins; D013634:Tannins; D009584:Nitrogen