title: Mature oocytes derived from purified mouse fetal germ cells.
abstract: BACKGROUND
  Mouse fetal germ cells have been successfully purified from fetal gonads. However, there are no published reports describing a procedure for deriving mature oocytes from isolated fetal germ cells. The purpose of this present study is to explore whether purified fetal germ cells are able to differentiate into mature oocytes through an in vivo grafting procedure.
  
  
  RESULTS
  First, intact 11.5 and 12.5 days post-coitum (dpc) female gonads with or without the attached mesonephros and the reaggregated female gonad cells were transplanted into the recipient mice. The results demonstrate both the gonad accompanied by mesonephroi and the innate gonad structure are not absolutely required for 11.5 dpc and 12.5 dpc oogonia to generate mature oocytes. Next, oogonia were purified from female gonads, aggregated with different ovarian somatic cells and transplanted into the recipient mice. Purified 12.5 dpc oogonia were able to generate mature oocytes by aggregating with 12.5 dpc ovarian somatic cells, but not with 16.5 dpc or 0 days postpartum ovarian somatic cells. We also tested 12.5 dpc male germ cells but they were unable to undergo oogenesis.
  
  
  CONCLUSIONS
  Our study demonstrates that mature oocytes can be derived from purified fetal germ cells through an aggregation and transplantation procedure. It also suggests that the synchronized interactions between oogonia and gonadal somatic cells are important to ensure normal folliculogenesis.
authors.affiliation: Laboratory of Stem Cell and Regeneration Biology, College of Life Sciences, Peking University, Beijing 100871, People's Rebublic of China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Qing
authors.forename: Haisong
authors.initials: H
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Xin
authors.initials: X
authors.lastname: Ye
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.forename: Donghui
authors.initials: D
authors.lastname: Zhang
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Song
authors.forename: Weifeng
authors.initials: W
authors.lastname: Yang
authors.forename: Mingxiao
authors.initials: M
authors.lastname: Ding
authors.forename: Hongkui
authors.initials: H
authors.lastname: Deng
mesh_terms: D000818:Animals; D002449:Cell Aggregation; D002454:Cell Differentiation; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D008297:Male; D008650:Mesonephros / Q000637:transplantation; D051379:Mice / Q000196:embryology*; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000502:physiology*; D009867:Oogonia / Q000166:cytology / Q000637:transplantation; D010053:Ovary / Q000196:embryology* / Q000637:transplantation; D016042:Transplantation, Heterotopic