[Adaptogenic effect of the vitamin D3 containing supplement "videchol" on glucose-6-phosphate dehydrogenase activity in erythrone of irradiated rats].
Two fast migrating, major, multiple molecular forms (MMF) of glucose-6-phosphate dehydrogenase [EC:1.1.1.49]: G-6-PDH-1 and G-6-PDH-2, and two minor forms: G-6-PDH-3 and G-6-PDH-4 were revealed in the electrophoregrams of both erythrocytes haemolisates as well in the homogenates of bone marrow cellular lines of rats at control conditions. Daily 1 cGy irradiation of rats up to a cumulative dose of 20 cGy led to a drop of G-6-PDH total activity and it caused a redistribution of the MMF of the enzyme in bone marrow cellular populations. However, G-6-PDH activity in erythrocytes exceeded the control means in all the experimental terms. The calculation of the local redistribution coefficient (l(G-6-FDH-i)) showed that these changes are mainly determined by the increase of the activity of the isoform G-6-PDH-3. Vitamin D3 administration to rats generated a correction of G-6-PDH activity in all studied cellular populations. Meanwhile, the MMF profiles were characterized by multidirectional rearrangements in the bone marrow erythroid and granulocyte-monocyte cells and in erythrocytes. The specificity of changes in the distribution of the MMF of G-6-PDH in the three studied cellular populations depends on the particularities of their energetic metabolism at irradiation conditions and on the modifying action of the natural adaptogen 1,25-dihydroxicholecalciferol.
Becerril Aragon
G A
GA
Starykovych
L S
LS
Kolodkin
A
A
Horot'
I V
IV
Velykyĭ
M M
MM
D000222:Adaptation, Physiological / Q000187:drug effects / Q000528:radiation effects; D000818:Animals; D002762:Cholecalciferol / Q000008:administration & dosage / Q000494:pharmacology*; D019587:Dietary Supplements; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D015672:Erythroid Precursor Cells / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D011829:Radiation Dosage; D051381:Rats; D014916:Whole-Body Irradiation
D002762:Cholecalciferol; D005954:Glucosephosphate Dehydrogenase