title: Microparticle-enhanced cultivation of filamentous microorganisms: increased chloroperoxidase formation by Caldariomyces fumago as an example.
abstract: Microparticle-enhanced cultivation (MPEC) was applied as a novel method for improved biomass and product formation during cultivation of filamentous microorganisms. Exemplarily, chloroperoxidase (CPO) formation by Caldariomyces fumago was analyzed in the presence and absence of microparticles of different size. Particles of approximately 500 microm in diameter had no effect on growth morphology or productivity of CPO formation by C. fumago. In contrast particles of < or =42 microm in diameter led to the dispersion of the C. fumago mycelia up to the level of single hyphae. Under these conditions the maximum specific productivity of CPO formation was enhanced about fivefold and an accumulated CPO activity in the culture supernatant of more than 1,000 U mL(-1) was achieved after 10-12 days of cultivation. In addition, the novel cultivation method also showed a positive effect on growth characteristics of other filamentous microorganisms proven by the stimulation of single hyphae/cell formation.
authors.affiliation: DECHEMA e.V, Karl-Winnacker-Institut, Biochemical Engineering Group, Theodor-Heuss-Allee 25, 60486 Frankfurt, Germany.
authors.forename: Bjoern-Arne
authors.initials: BA
authors.lastname: Kaup
authors.forename: Kristina
authors.initials: K
authors.lastname: Ehrich
authors.forename: Michael
authors.initials: M
authors.lastname: Pescheck
authors.forename: Jens
authors.initials: J
authors.lastname: Schrader
mesh_terms: D001203:Ascomycota / Q000201:enzymology* / Q000254:growth & development*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D002711:Chloride Peroxidase / Q000378:metabolism*; D008863:Microspheres
chemical_list: D002711:Chloride Peroxidase