HLA-B*1301 as a biomarker for genetic susceptibility to hypersensitivity dermatitis induced by trichloroethylene among workers in China.
BACKGROUND
Trichloroethylene (TCE) is used extensively as an industrial solvent and has been recognized as one of the major environmental pollutants. To date, > 200 cases of TCE-induced hypersensitivity dermatitis among exposed workers have been reported worldwide, and TCE exposure has become one of the critical occupational health issues in Asia.


OBJECTIVE
The study aimed to identify genetic susceptible biomarkers associated with the TCE-induced hypersensitivity dermatitis in genes located in the human leukocyte antigen (HLA) region.


METHODS
From 1998 to 2006, 121 cases with TCE-induced hypersensitivity dermatitis and 142 tolerant controls were recruited into the population-based case-control study. We determined HLA alleles B, DRB1, DQA1, and DQB1, by sequence-based typing. p-Values were corrected for comparisons of multiple HLA alleles. In addition, we compared and analyzed the structure character of amino acid residues of HLA molecules found in participants.


RESULTS
We obtained complete genotyping data of 113 cases and 142 controls. The allele HLA-B*1301 was present in 83 (73.5%) of 113 patients compared with 13 (9.2%) of 142 tolerant workers (odds ratio = 27.5; 95% confidence interval, 13.5-55.7; corrected p = 1.48 x 10(-21)). In addition, the HLA-B*44 alleles were present in 6.2% (7/113) of patients, but were absent in TCE-tolerant workers. Residue 95 shared by HLA-B*1301 and HLA-B*44 molecules formed a different pocket F than other residues.


CONCLUSIONS
The allele HLA-B*1301 is strongly associated with TCE-induced hypersensitivity dermatitis among exposed workers and might be used as a biomarker to predict high risk individuals to TCE.
Li
Haishan
H
Key Laboratory, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China.
Dai
Yufei
Y
Huang
Hanlin
H
Li
Laiyu
L
Leng
Shuguang
S
Cheng
Juan
J
Niu
Yong
Y
Duan
Huawei
H
Liu
Qingjun
Q
Zhang
Xing
X
Huang
Xianqing
X
Xie
Jinxin
J
Feng
Zhiming
Z
Wang
Juncai
J
He
Jiaxi
J
Zheng
Yuxin
Y
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002681:China; D003872:Dermatitis / Q000209:etiology / Q000235:genetics*; D004342:Drug Hypersensitivity / Q000209:etiology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D015235:HLA-B Antigens / Q000737:chemistry / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D008958:Models, Molecular; D016273:Occupational Exposure; D011110:Polymorphism, Genetic; D014241:Trichloroethylene / Q000009:adverse effects*
D015235:HLA-B Antigens; D014241:Trichloroethylene