Neoplastic transformation and induction of H+,K+ -adenosine triphosphatase by N-methyl-N'-nitro-N-nitrosoguanidine in the gastric epithelial RGM-1 cell line.
N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induces gastric cancer in animal models. We established an MNNG-induced mutant of the rat murine RGM-1 gastric epithelial cell line, which we named RGK-1, that could be used as an in vitro model of gastric cancer. This cell line showed signs of neoplasia and transformation, in that it lost contact inhibition and formed tumors in nude mice. The mutant cells also expressed parietal cell-specific H(+),K(+)-adenosine triphosphatase (H(+),K(+)-ATPase), which parent RGM-1 did not. The results suggested that parent RGM-1 cells were gastric progenitor cells. This mutant RGK-1 cell line will contribute to future investigation on gastric carcinogenesis and to the development of other pathophysiologic fields.
Shimokawa
Osamu
O
Division of Gastroenterology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Ten-nohdai, Tsukuba, Ibaraki, Japan.
Matsui
Hirofumi
H
Nagano
Yumiko
Y
Kaneko
Tsuyoshi
T
Shibahara
Takeshi
T
Nakahara
Akira
A
Hyodo
Ichinosuke
I
Yanaka
Akinori
A
Majima
Hideyuki J
HJ
Nakamura
Yukio
Y
Matsuzaki
Yasushi
Y
D000818:Animals; D002273:Carcinogens / Q000378:metabolism; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004847:Epithelial Cells / Q000502:physiology* / Q000648:ultrastructure; D005753:Gastric Mucosa / Q000166:cytology*; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism*; D008769:Methylnitronitrosoguanidine / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D002273:Carcinogens; D008769:Methylnitronitrosoguanidine; D017506:H(+)-K(+)-Exchanging ATPase