title: Selective regulation of mutant K-ras mRNA expression by photo-cross-linking antisense oligonucleotide.
abstract: It has been reported that point mutations in genes are responsible for various cancers and the selective regulation of the gene expression is an important issue to develop new types of anticancer drugs. In this report, we report new types of antisense molecules that photo-cross-link to target mRNA having a point mutation site in a sequence specific manner. We designed and synthesized photo-reactive antisense oligonucleotides containing a 2'-O-psoralen-conjugated adenosine (2'-Ps-oligo). They contain a psoralen via an ethoxymethylene linker (2'-Ps-eom), a propoxymethylene linker (2'-Ps-pom) and a buthoxymethylene linker (2'-Ps-bom), respectively. We evaluated the photo-cross-linking efficiency and the sequence specificity toward complementary RNA. 2'-Ps-eom showed the highest photo-cross-linking efficiency among 2'-Ps-oligos.
authors.affiliation: Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto606-8585, Japan.
authors.forename: Maiko
authors.initials: M
authors.lastname: Higuchi
authors.forename: Asako
authors.initials: A
authors.lastname: Yamayoshi
authors.forename: Akio
authors.initials: A
authors.lastname: Kobori
authors.forename: Akira
authors.initials: A
authors.lastname: Murakami
mesh_terms: D000241:Adenosine / Q000737:chemistry; D011564:Furocoumarins / Q000138:chemical synthesis / Q000737:chemistry / Q000528:radiation effects; D005786:Gene Expression Regulation; D011905:Genes, ras; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000528:radiation effects*; D017354:Point Mutation; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D011564:Furocoumarins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D000241:Adenosine