title: Study on the kinetics of keto-enol tautomerism of p-hydroxyphenylpyruvic acid using capillary electrophoresis.
abstract: The kinetics of keto-enol tautomerism of p-hydroxyphenylpyruvic acid (pHPP) as a model of alpha-carbonyl compounds in aqueous solution at room temperature (25 degrees C) was first investigated by capillary electrophoresis with UV detection at 200 nm. The two tautomers could be separated and detected within 3 min. Since the ketonization of enolic pHPP varied with the buffer composition and buffer pH, the kinetics of pHPP was studied under different conditions, and relevant distributing fractions of enolic pHPP, ketonization rate constants and half-life were determined. In addition, beta-CD played an important part in the separation of the two tautomers, thus, the interaction between pHPP and beta-CD was also investigated by electrochemical techniques.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Fuzhou, Fujian 350002, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Yuwu
authors.initials: Y
authors.lastname: Chi
authors.forename: Jinming
authors.initials: J
authors.lastname: Lin
authors.forename: Lishuang
authors.initials: L
authors.lastname: Yu
authors.forename: Liangjun
authors.initials: L
authors.lastname: Xu
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D007536:Isomerism; D007700:Kinetics; D010667:Phenylpyruvic Acids / Q000737:chemistry*; D015203:Reproducibility of Results; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D010667:Phenylpyruvic Acids; D047392:beta-Cyclodextrins; C010590:4-hydroxyphenylpyruvic acid; C031215:betadex