Is phospholipid-saturated alkyl column a convenient replacement for immobilized-artificial-membrane?
Three phosphatidylcholine (PC)-saturated C(8)/C(18) stationary phases prepared using biologically representative membrane lipids (purchasable L-alpha-PC and pure 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine) have been developed to compare with IAM (immobilized-artificial-membrane) and C(8)/C(18) columns. These PC-coated stationary phases were found to be stable and reproducible for retention experiments. The retention characteristics of nucleobases on these coated phases deviate significantly from those on the IAM counterpart, but surprisingly similar to those of the underlying C(8)/C(18) columns. An inter-phase model has been proposed and explored for interpretation of the results.
Luo
Hai-Bin
HB
School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510080, China.
Zheng
Chuanqi
C
Cheng
Yuen-Kit
YK
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008567:Membranes, Artificial; D010743:Phospholipids / Q000737:chemistry*; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
D008567:Membranes, Artificial; D010743:Phospholipids