title: Neoplastic transformation and induction of H+,K+ -adenosine triphosphatase by N-methyl-N'-nitro-N-nitrosoguanidine in the gastric epithelial RGM-1 cell line.
abstract: N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induces gastric cancer in animal models. We established an MNNG-induced mutant of the rat murine RGM-1 gastric epithelial cell line, which we named RGK-1, that could be used as an in vitro model of gastric cancer. This cell line showed signs of neoplasia and transformation, in that it lost contact inhibition and formed tumors in nude mice. The mutant cells also expressed parietal cell-specific H(+),K(+)-adenosine triphosphatase (H(+),K(+)-ATPase), which parent RGM-1 did not. The results suggested that parent RGM-1 cells were gastric progenitor cells. This mutant RGK-1 cell line will contribute to future investigation on gastric carcinogenesis and to the development of other pathophysiologic fields.
authors.affiliation: Division of Gastroenterology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Ten-nohdai, Tsukuba, Ibaraki, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Shimokawa
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Matsui
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Nagano
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kaneko
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shibahara
authors.forename: Akira
authors.initials: A
authors.lastname: Nakahara
authors.forename: Ichinosuke
authors.initials: I
authors.lastname: Hyodo
authors.forename: Akinori
authors.initials: A
authors.lastname: Yanaka
authors.forename: Hideyuki J
authors.initials: HJ
authors.lastname: Majima
authors.forename: Yukio
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Matsuzaki
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000378:metabolism; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004847:Epithelial Cells / Q000502:physiology* / Q000648:ultrastructure; D005753:Gastric Mucosa / Q000166:cytology*; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism*; D008769:Methylnitronitrosoguanidine / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002273:Carcinogens; D008769:Methylnitronitrosoguanidine; D017506:H(+)-K(+)-Exchanging ATPase