Analysis of molecular inversion probe performance for allele copy number determination.
We have developed a new protocol for using molecular inversion probes to accurately and specifically measure allele copy number. The new protocol provides for significant improvements, including the reduction of input DNA (from 2 mug) by more than 25-fold (to 75 ng total genomic DNA), higher overall precision resulting in one order of magnitude lower false positive rate, and greater dynamic range with accurate absolute copy number up to 60 copies.
Wang
Yuker
Y
Affymetrix Inc, Shoreline Blvd, South San Francisco, CA 94080, USA. Yuker_Wang@affymetrix.com
Moorhead
Martin
M
Karlin-Neumann
George
G
Wang
Nicholas J
NJ
Ireland
James
J
Lin
Steven
S
Chen
Chunnuan
C
Heiser
Laura M
LM
Chin
Koei
K
Esserman
Laura
L
Gray
Joe W
JW
Spellman
Paul T
PT
Faham
Malek
M
D000483:Alleles; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D007446:Chromosome Inversion; D005189:False Positive Reactions; D006801:Humans; D019656:Loss of Heterozygosity; D015335:Molecular Probes; D016133:Polymerase Chain Reaction; D012372:ROC Curve
D015335:Molecular Probes