Quantitative analysis of auxin-regulated proteins from basal part of leaf sheaths in rice by two-dimensional difference gel electrophoresis.
To identify the effects of auxin on rice root formation, proteins induced by exogenous addition of auxin to rice seedlings were analyzed by a proteomic approach. Root formation by rice seedlings was promoted by 0.45microM 2,4-dichlorophenoxyacetic acid (2,4-D) and repressed by 60microM p-chlorophenoxyisobutyric acid (PCIB). Proteins extracted from the basal part of leaf sheaths of rice seedlings treated with 2,4-D or PCIB for 48h were labeled with Cy3 and Cy5, and separated by two-dimensional polyacrylamide gel electrophoresis. Out of nine proteins up-regulated by 2,4-D and down-regulated by PCIB, five proteins showing significant difference in abundance were used for expression analysis at the transcript abundance level. Transcript abundance of the mitochondrial complex I subunit slightly increased with 2,4-D treatment and were repressed by PCIB. The transcript abundance of EF-1beta', myosin heavy chain and mitochondrial [Mn]SOD increased with 2,4-D treatment but did not decrease with PCIB. The transcript abundance of aldehyde dehydrogenase was not effected by 2,4-D or PCIB. These results indicate that mitochondrial complex I subunit is part of the downstream signal cascade of PCIB, whereas myosin heavy chain, mitochondrial [Mn]SOD and EF-1beta' are involved in the 2,4-D signal cascade but are probably upstream of PCIB.
Shi
Fang
F
National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba 305-8518, Japan.
Takasaki
Hironori
H
Komatsu
Setsuko
S
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D002995:Clofibric Acid / Q000494:pharmacology; D042967:Electron Transport Complex I / Q000187:drug effects / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D007210:Indoleacetic Acids / Q000494:pharmacology*; D009218:Myosins / Q000187:drug effects / Q000235:genetics; D012275:Oryza / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D020648:Peptide Elongation Factor 1 / Q000187:drug effects / Q000235:genetics; D018515:Plant Leaves / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D040901:Proteomics; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D012680:Sensitivity and Specificity; D013482:Superoxide Dismutase / Q000187:drug effects / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
D007210:Indoleacetic Acids; D020648:Peptide Elongation Factor 1; D010940:Plant Proteins; D015084:2,4-Dichlorophenoxyacetic Acid; D002995:Clofibric Acid; D013482:Superoxide Dismutase; D009218:Myosins; D042967:Electron Transport Complex I