title: Ce(IV)-catalyzed site-selective DNA hydrolysis using tris- and tetrakismethylenephosphonate-modified oligonucleotides.
abstract: To improve the recruitment of the catalytically active Ce(IV) ion to the DNA cleavage site in the ARCUT (Artificial Restriction DNA Cutter) system, two phosphonate ligands, nitrilotris(methylenephosphonic acid) (NTP) and N,N,N',N'-ethylenediaminetetra-(methylenephosphonic acid) (EDTP), have been linked to the 5'- or 3'-end of oligonucleotides complementary to the target DNA. Compared to their counterparts bearing a terminal monophosphate, these new modified oligonucleotides exhibit a modest to significant increase in the cleavage activity, depending on the target.
authors.affiliation: RCAST, The University of Tokyo, 4-6-1, Komaba, Meguro-Ku, Tokyo 153-8904, Japan. tuanlo@utu.fi
authors.forename: Tuomas
authors.initials: T
authors.lastname: Lönnberg
authors.forename: Yuta
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Makoto
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D002384:Catalysis; D002563:Cerium / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005029:Ethylenediamines / Q000737:chemistry*; D006868:Hydrolysis; D009841:Oligonucleotides / Q000737:chemistry*; D063065:Organophosphonates / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry*
chemical_list: D005029:Ethylenediamines; D009841:Oligonucleotides; D063065:Organophosphonates; D009943:Organophosphorus Compounds; C418473:ethylenediaminetetrakis(methylphosphonic) acid; C041182:(nitrilotris(methylene))triphosphonic acid; D002563:Cerium; D004247:DNA