Delivery of dsRNA with lactic acid bacteria for RNA interference.
RNA interference (RNAi) mechanism is promising for gene-targeted therapy. In this study, Lactobacillus casei were engineered to produce long double-stranded RNA (dsRNA) or short hairpin RNA (shRNA) for RNAi-delivery to the intestine. We prepared two kinds of L. casei; one produces shRNA which works in mammalian cells, and the other produces long dsRNA which works in nematode. We confirmed the expression of shRNA and dsRNA in the transformed L. casei by Northern blotting and RT-PCR analyses.
Kuwahara
Ai
A
Department of Bioscience and Biotechnology, Okayama-University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
Arita
Masashi
M
Sisido
Masahiko
M
Ohtsuki
Takashi
T
D000284:Administration, Oral; D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism; D005818:Genetic Engineering; D005822:Genetic Vectors; D007780:Lacticaseibacillus casei / Q000235:genetics*; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000008:administration & dosage / Q000096:biosynthesis*; D022661:RNA, Untranslated / Q000096:biosynthesis / Q000737:chemistry
D012330:RNA, Double-Stranded; D022661:RNA, Untranslated