title: Induced pluripotent stem cell lines derived from human somatic cells.
abstract: Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations (for example, mutation through viral integration) are eliminated.
authors.affiliation: Genome Center of Wisconsin, Madison, WI 53706-1580, USA. jyu@primate.wisc.edu
authors.forename: Junying
authors.initials: J
authors.lastname: Yu
authors.forename: Maxim A
authors.initials: MA
authors.lastname: Vodyanik
authors.forename: Kim
authors.initials: K
authors.lastname: Smuga-Otto
authors.forename: Jessica
authors.initials: J
authors.lastname: Antosiewicz-Bourget
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Frane
authors.forename: Shulan
authors.initials: S
authors.lastname: Tian
authors.forename: Jeff
authors.initials: J
authors.lastname: Nie
authors.forename: Gudrun A
authors.initials: GA
authors.lastname: Jonsdottir
authors.forename: Victor
authors.initials: V
authors.lastname: Ruotti
authors.forename: Ron
authors.initials: R
authors.lastname: Stewart
authors.forename: Igor I
authors.initials: II
authors.lastname: Slukvin
authors.forename: James A
authors.initials: JA
authors.lastname: Thomson
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D048430:Cell Shape; D065150:Cellular Reprogramming; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D053595:Embryonic Stem Cells / Q000166:cytology; D005333:Fetus; D005347:Fibroblasts / Q000166:cytology*; D024002:HMGB Proteins / Q000235:genetics / Q000502:physiology; D018398:Homeodomain Proteins / Q000235:genetics / Q000502:physiology; D006801:Humans; D007231:Infant, Newborn; D007621:Karyotyping; D051379:Mice; D016513:Mice, SCID; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D055748:SOXB1 Transcription Factors; D033581:Stem Cell Transplantation; D013724:Teratoma / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology; D014161:Transduction, Genetic; D019076:Transgenes
chemical_list: D004268:DNA-Binding Proteins; D024002:HMGB Proteins; D018398:Homeodomain Proteins; C000715187:Lin28A protein, human; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; D016601:RNA-Binding Proteins; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; D014157:Transcription Factors