title: Enhancing effect of chlorinated organic solvents on histamine release and inflammatory mediator production.
abstract: We investigated the effect of several chlorinated organic solvents on antigen-induced histamine release and inflammatory mediator production. Non-purified rat peritoneal mast cells (NPMC) and rat basophilic leukemia (RBL-2H3) cells were sensitized with anti-dinitrophenol (DNP) monoclonal IgE antibody, and then stimulated with DNP-conjugated bovine serum albumin (DNP-BSA) and several chlorinated organic solvents. Trichloroethylene (TCE) and tetrachloroethylene (PCE) enhanced histamine release from antigen-stimulated NPMC and RBL-2H3 in a dose-dependent manner. In addition, TCE and PCE increased IL-4 and TNF-alpha production from antigen-stimulated RBL-2H3. In an in vivo study, we investigated the effect of TCE and PCE on passive cutaneous anaphylaxis (PCA) reaction. TCE and PCE enhanced PCA reaction markedly. These results suggest that TCE and PCE increase histamine release and inflammatory mediator production from antigen-stimulated mast cells via the modulation of immune responses. In addition, exposure to TCE and PCE may lead to the augmentation of allergic diseases.
authors.affiliation: Laboratory of Hygienics, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, Japan. mkt.s.lev5060@gmail.com
authors.forename: Makoto
authors.initials: M
authors.lastname: Seo
authors.forename: Koji
authors.initials: K
authors.lastname: Ikeda
authors.forename: Tetsunori
authors.initials: T
authors.lastname: Okamura
authors.forename: Kumiko
authors.initials: K
authors.lastname: Kida
authors.forename: Masahiko
authors.initials: M
authors.lastname: Satoh
authors.forename: Naoki
authors.initials: N
authors.lastname: Inagaki
authors.forename: Hiroichi
authors.initials: H
authors.lastname: Nagai
authors.forename: Hisamitsu
authors.initials: H
authors.lastname: Nagase
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D045744:Cell Line, Tumor; D004140:Dinitrophenols / Q000276:immunology / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006636:Histamine Release / Q000187:drug effects* / Q000276:immunology; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity*; D018836:Inflammation Mediators / Q000276:immunology*; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D012710:Serum Albumin, Bovine / Q000276:immunology / Q000494:pharmacology; D012997:Solvents / Q000633:toxicity*
chemical_list: D000911:Antibodies, Monoclonal; D004140:Dinitrophenols; D006843:Hydrocarbons, Chlorinated; D018836:Inflammation Mediators; D012997:Solvents; C034638:dinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine