title: Lipid analysis of normal dermis and hypertrophic scars.
abstract: Hypertrophic scars (HS) are a consequence of abnormal wound healing. We examined fatty acids that are contained within, and participate in, every reaction through the membrane; then, we analyzed the percentage composition of the fatty acids in deepithelialized normal dermis (ND) and HS. In vivo HS samples were obtained from six patients undergoing surgical excision, and ND samples from five patients undergoing skin grafting surgery for excess. In vitro, cultured fibroblasts from HS and ND were also analyzed. The percentage composition of fatty acids extracted from all the samples was analyzed. In vivo, arachidonic acid (20:4) was significantly more abundant in HS than in ND, in the phospholipids from both whole tissue and cell membranes. In vitro, there were no significant differences among ND, HS, and 10% fetal calf serum. The results suggest that HS formation does not necessarily involve simple excess of 20:4; however, there are considerable differences in the percentage composition of 20:4 between ND and HS. Arachidonic acid probably participates in the formation and maintenance of HS, whereas in vitro cultured fibroblasts are affected largely by fetal calf serum.
authors.affiliation: Department of Plastic Surgery [corrected], Kobe University Graduate School of Medicine, Kobe, Japan. tadnomura-tky@umin.ac.jp
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nomura
authors.forename: Hiroto
authors.initials: H
authors.lastname: Terashi
authors.forename: Makoto
authors.initials: M
authors.lastname: Omori
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sakurai
authors.forename: Takeru
authors.initials: T
authors.lastname: Sunagawa
authors.forename: Masumi
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Shinya
authors.initials: S
authors.lastname: Tahara
mesh_terms: D016718:Arachidonic Acid / Q000032:analysis; D017439:Cicatrix, Hypertrophic / Q000378:metabolism*; D020405:Dermis / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D010743:Phospholipids / Q000032:analysis
chemical_list: D005227:Fatty Acids; D010743:Phospholipids; D016718:Arachidonic Acid