title: Rapid and highly sensitive coxsackievirus a indirect immunofluorescence assay typing kit for enterovirus serotyping.
abstract: We describe the development and evaluation of an indirect immunofluorescence assay (IFA) kit for rapid and sensitive detection of coxsackievirus A2, -4, -5, -6, and -10. This IFA kit was determined to have 95.9 to 100% sensitivity and 95.8 to 97.2% specificity. It also proved to be beneficial in reducing the number of enteroviruses that are untypeable in the clinical virology laboratory.
authors.affiliation: Center for Research and Diagnostic, Centers for Disease Control, Department of Health, Taiwan, 161 Kunyang Street, Nan-Kang, Taipei, Taiwan.
authors.forename: Tsuey-Li
authors.initials: TL
authors.lastname: Lin
authors.forename: Yi-Syue
authors.initials: YS
authors.lastname: Li
authors.forename: Chiao-Wei
authors.initials: CW
authors.lastname: Huang
authors.forename: Chiu-Chu
authors.initials: CC
authors.lastname: Hsu
authors.forename: Ho-Sheng
authors.initials: HS
authors.lastname: Wu
authors.forename: Tsan-Chang
authors.initials: TC
authors.lastname: Tseng
authors.forename: Chen-Fu
authors.initials: CF
authors.lastname: Yang
mesh_terms: D003384:Coxsackievirus Infections / Q000175:diagnosis* / Q000821:virology*; D004770:Enterovirus / Q000145:classification*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity