title: Clinical relevance of Helicobacter pylori sabA genotype in Japanese clinical isolates.
abstract: OBJECTIVE
  The clinical outcome of Helicobacter pylori infection is strongly affected by the bacterial genotype. The aim of this study was to assess the relationship between the status of H. pylori sialic acid-binding adhesin (sabA) and the severity of gastric inflammation and diseases.
  
  
  METHODS
  Clinical isolates from 108 patients with duodenal ulcer, gastric ulcer, gastric cancer, or chronic gastritis were analyzed by sequencing and PCR assay. The degree of neutrophil infiltration in the antral region was evaluated by histopathological examination. The extent of atrophic change was evaluated using serum pepsinogen levels and the pepsinogen I/II ratio.
  
  
  RESULTS
  Functional sabA was present in 88 (81%) of the 108 patients. Although there was no correlation between the sabA status and gastric diseases, severe neutrophil infiltration and atrophy were associated with functional sabA.
  
  
  CONCLUSIONS
  The results show a high prevalence of functional sabA in Japanese clinical isolates. The status of sabA may be an important virulence factor in inducing chronic gastric inflammation.
authors.affiliation: Division of Gastroenterology, The Institute for Adult Diseases, Asahi Life Foundation, Chiyoda-ku, Tokyo, Japan. ayanai-gi@umin.ac.jp
authors.forename: Ayako
authors.initials: A
authors.lastname: Yanai
authors.forename: Shin
authors.initials: S
authors.lastname: Maeda
authors.forename: Yoko
authors.initials: Y
authors.lastname: Hikiba
authors.forename: Wataru
authors.initials: W
authors.lastname: Shibata
authors.forename: Tomoya
authors.initials: T
authors.lastname: Ohmae
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hirata
authors.forename: Keiji
authors.initials: K
authors.lastname: Ogura
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Yoshida
authors.forename: Masao
authors.initials: M
authors.lastname: Omata
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D044466:Asian People; D001284:Atrophy; D001483:Base Sequence; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology; D005838:Genotype; D016481:Helicobacter Infections / Q000453:epidemiology; D016480:Helicobacter pylori / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D007249:Inflammation / Q000382:microbiology; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010435:Pepsinogens / Q000097:blood; D015995:Prevalence; D037521:Virulence Factors
chemical_list: D018829:Adhesins, Bacterial; D010435:Pepsinogens; C500808:SabA protein, Helicobacter pylori; D037521:Virulence Factors