Sensitive and rapid detection of Aeromonas caviae in stool samples by loop-mediated isothermal amplification.
In this study, a loop-mediated isothermal amplification assay targeting the 16S-23S intergenic spacer regions (internal transcribed spacer) of Aeromonas caviae was developed. Eighteen reference strains and 109 clinical samples were analyzed. The results showed this detection technique is more reliable and convenient compared with common polymerase chain reaction and biochemical culture methods.
Wei
Xiao Na
XN
Department of Biochemistry and Molecular Biology, College of Life Science, Nankai University, Tianjin 300071, China.
Zheng
Ze Jun
ZJ
Zhang
Li Huan
LH
Qu
Fen
F
Huang
Xitai
X
D000333:Aeromonas / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D021901:DNA, Intergenic