Hyperproduction of sebaceous cis-6-hexadecenoic acid by esterase-reduced mutant of Rhodococcus sp. strain.
cis-6-Hexadecenoic acid is a major component of human sebaceous lipids that is involved in skin self-sterilization and atopic dermatitis amelioration. It can be prepared by hydrolysis of isopropyl cis-6-hexadecenoate produced by resting cells of Rhodococcus sp. strain KSM-MT66. To devise an economical industrial-scale process for the production of this rare fatty acid, we optimized the conditions for growing rhodococcal cells. Mg(2+) and Fe(2+) ions are essential for the efficient production of isopropyl cis-6-hexadecenoate. To further increase the production of isopropyl cis-6-hexadecenoate, we created a mutant strain (T64) with reduced esterase activity by random mutagenesis using UV irradiation of MT66. Under an optimized condition, the mutant T64 produced more than 60 g l(-1) isopropyl cis-6-hexadecenoate in a 4-d cultivation, corresponding to about 52 g l(-1)cis-6-hexadecenoate.
Araki
Hiroyuki
H
Tochigi Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi 321-3497, Japan. araki.hiroyuki@kao.co.jp
Hagihara
Hiroshi
H
Takigawa
Hirofumi
H
Kotani
Nobuharu
N
Tsujino
Yukiharu
Y
Koike
Kenzo
K
Kawai
Shuji
S
Ozaki
Katsuya
K
Ito
Susumu
S
D004950:Esterases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010169:Palmitic Acids / Q000378:metabolism*; D012240:Rhodococcus / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D013045:Species Specificity; D015854:Up-Regulation
D010169:Palmitic Acids; C093821:hexadecenoic acid; D004950:Esterases