title: Comparison of BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR versus the CHROMagar MRSA assay for screening patients for the presence of MRSA strains.
abstract: We compared the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) real-time PCR assay with the CHROMagar MRSA assay for the detection of MRSA in 286 nasal surveillance specimens. Compared with the CHROMagar MRSA assay, PCR had sensitivity, specificity, positive predictive value, and negative predictive values of 100%, 98.6%, 95.8%, and 100%, respectively. The mean PCR turnaround time was 14.5 h.
authors.affiliation: Department of Medicine, Hospital of Saint Raphael, 1450 Chapel Street, New Haven, Connecticut 06511, USA. JBoyce@srhs.org
authors.forename: John M
authors.initials: JM
authors.lastname: Boyce
authors.forename: Nancy L
authors.initials: NL
authors.lastname: Havill
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002353:Carrier State / Q000175:diagnosis / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry; D006784:Hospitals, Teaching; D006801:Humans; D016106:Methicillin Resistance; D009666:Nose / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D003470:Culture Media