title: Microsatellite-centromere mapping in large yellow croaker (Pseudosciaena crocea) using gynogenetic diploid families.
abstract: The large yellow croaker (Pseudosciaena crocea) is an economically important marine fish in China. Inheritance of 22 heterozygous microsatellite loci was examined in normal crossed diploid families and meio-gynogenetic families in P. crocea. Two gynogenetic families were produced via inhibition of the second polar body in eggs fertilized with UV-irradiated sperm. The ratio of gynogenesis was proven to be 100% and 96.9% in the two families, respectively. Of the 22 examined loci, 4 showed a segregation distortion in both control and gynogenetic families. Microsatellite-centromere (M-C) map distances were examined using 18 loci with normal Mendelian segregation. Estimated recombination rates ranged between 0 and 1.0 under the assumption of complete interference. High recombinant frequencies between heterozygous markers and the centromere were found in large yellow croaker, as in other teleosts. The average recombination frequency was 0.586. Ten loci showed high M-C recombination with frequency greater than 0.67. M-C distances provide useful information for gene mapping in large yellow croaker.
authors.affiliation: The Key Laboratory of Science and Technology for Aquaculture and Food Safety of Fujian Province University, Fisheries College, Jimei University, Xiamen, China.
authors.forename: Yiyun
authors.initials: Y
authors.lastname: Li
authors.forename: Mingyi
authors.initials: M
authors.lastname: Cai
authors.forename: ZhiYong
authors.initials: Z
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Xiande
authors.initials: X
authors.lastname: Liu
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Ning
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods; D002503:Centromere / Q000235:genetics*; D002681:China; D002874:Chromosome Mapping / Q000379:methods*; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005838:Genotype; D040582:Inheritance Patterns / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D010473:Perciformes / Q000235:genetics*
chemical_list: D017931:DNA Primers