Development of high-sensitive DNA probe by using perylene.
Modified oligodeoxyribonucleotides (ODNs) involving two perylene moieties are synthesized. By using this ODN, one-base deletion can easily be distinguished with high sensitivity. In addition, emission color of the solution greatly changed so that the detection was possible even by naked eyes.
Kashida
Hiromu
H
Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Takatsu
Tomohiko
T
Asanuma
Hiroyuki
H
D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D010569:Perylene / Q000737:chemistry*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D013050:Spectrometry, Fluorescence
D015342:DNA Probes; D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D010569:Perylene