title: Purification, crystallization and preliminary X-ray diffraction study of human ribosomal protein L10 core domain.
abstract: Eukaryotic ribosomal protein L10 is an essential component of the large ribosomal subunit, which organizes the architecture of the aminoacyl-tRNA binding site. The human L10 protein is also called the QM protein and consists of 214 amino-acid residues. For crystallization, the L10 core domain (L10CD, Phe34-Glu182) was recombinantly expressed in Escherichia coli and purified to homogeneity. A hexagonal crystal of L10CD was obtained by the sitting-drop vapour-diffusion method. The L10CD crystal diffracted to 2.5 A resolution and belongs to space group P3(1)21 or P3(2)21.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Nishimura
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kaminishi
authors.forename: Masahito
authors.initials: M
authors.lastname: Kawazoe
authors.forename: Mikako
authors.initials: M
authors.lastname: Shirouzu
authors.forename: Chie
authors.initials: C
authors.lastname: Takemoto
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Akiko
authors.initials: A
authors.lastname: Tanaka
authors.forename: Sumio
authors.initials: S
authors.lastname: Sugano
authors.forename: Takuya
authors.initials: T
authors.lastname: Yoshida
authors.forename: Tadayasu
authors.initials: T
authors.lastname: Ohkubo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kobayashi
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D000080882:Ribosomal Protein L10; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D025521:Tumor Suppressor Proteins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: C000630686:RPL10 protein, human; D012269:Ribosomal Proteins; D025521:Tumor Suppressor Proteins