Effects of isoflurane exposure on oncogene and tumour suppressor gene expressions in vital organs of CBA/CA mice.
BACKGROUND
Isoflurane is a halogenated volatile anesthetic. It is generally believed that modern inhaled anesthetics are not themselves toxic but their intermediates might be. Whether isoflurane exposure results in any alteration in oncogene and tumour suppressor gene expression was investigated here.


METHODS
CBA/CA H-2(k) mice were anesthetized with isoflurane for 1 hour. The expression alterations of c-myc, Ha-ras and p53 genes were investigated in the vital organs 3, 24 and 48 hours later.


RESULTS
Expression patterns of the investigated genes differed both in magnitude and time course according to gender. Overexpressions were detected after 24 hours in both sexes, but in females, overexpression of all investigated genes remained high in all organs at 48 hours, except in the bone-marrow.


CONCLUSIONS
Considerable overexpression of two oncogenes and a tumour suppressor gene after isoflurane exposure were found.
Kádár
Balázs
B
Department of Anesthesiology and Intensive Therapy, Borsod County Hospital, 3501, Miskolc, Hungary. kadarbalazsdr@gmail.com
Gombos
Katalin
K
Szele
Eszter
E
Beregi
Attila
A
Varga
Zsuzsanna
Z
Sebestyén
Andor
A
Ember
István
I
D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000235:genetics*; D007530:Isoflurane / Q000494:pharmacology*; D051379:Mice; D015513:Oncogene Proteins / Q000235:genetics*; D009928:Organ Specificity; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D015513:Oncogene Proteins; D025521:Tumor Suppressor Proteins; D007530:Isoflurane