title: Experimental infection of dogs with a novel strain of canine coronavirus causing systemic disease and lymphopenia.
abstract: A pantropic canine coronavirus (CCoV) strain (CB/05) has been recently associated to a fatal outbreak of systemic disease in young dogs. We report the clinical, virological and serological findings in dogs experimentally infected with strain CB/05. The dogs, three 2.5-month-old and two 6-month-old pups, were successfully infected, shedding viral RNA with their faeces for the entire observation period (21 days) and displaying systemic clinical signs resembling those observed during the course of natural infection. Leucopenia (acute lymphopenia) occurred in all infected dogs, with values dropping below 60% of the initial counts. Considering the severity of the CB/05-induced disease, two of the youngest pups were euthanized for ethical reasons at days 8-9 postinfection, whereas the other pups underwent a slow but progressive improvement of their clinical status with complete recovery. At postmortem examination, remarkable lesions were observed in the internal organs of the euthanized pups, that tested positive for CCoV by real-time RT-PCR and virus isolation on cell cultures. All pups seroconverted for CCoV, as shown by the high optical density values and antibody titres detected by ELISA and virusneutralisation tests, respectively. The present study confirms that strain CB/05 is highly pathogenic for dogs, being able to induce a severe disease (and in some cases the death) even in experimental conditions.
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima km 3, 70010 Valenzano, Bari, Italy. n.decaro@veterinaria.uniba.it
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
authors.forename: Marco
authors.initials: M
authors.lastname: Campolo
authors.forename: Alessio
authors.initials: A
authors.lastname: Lorusso
authors.forename: Costantina
authors.initials: C
authors.lastname: Desario
authors.forename: Viviana
authors.initials: V
authors.lastname: Mari
authors.forename: Maria Loredana
authors.initials: ML
authors.lastname: Colaianni
authors.forename: Gabriella
authors.initials: G
authors.lastname: Elia
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood*; D018352:Coronavirus Infections / Q000097:blood / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D017939:Coronavirus, Canine / Q000276:immunology / Q000472:pathogenicity*; D004283:Dog Diseases / Q000097:blood / Q000401:mortality / Q000473:pathology / Q000821:virology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005243:Feces / Q000821:virology; D005260:Female; D007970:Leukopenia / Q000097:blood / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D009500:Neutralization Tests / Q000379:methods / Q000662:veterinary; D009928:Organ Specificity; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012720:Severity of Illness Index
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral