A new water-soluble near-neutral ratiometric fluorescent pH indicator.
Donor-pi-acceptor fluorene derivative 1c is a near-neutral pH indicator whose pKa of approximately 7.0 was determined by both absorption and fluorescence methods. 1c satisfies important criteria for a sensitive ratiomeric fluorescent pH indicator with a distinctive isoemissive point, good dispersion in cell cytosol, and low cytotoxicity. Furthermore, its 2PA cross section of 100 GM in its neutral form suggests its potential in two-photon fluorescence imaging applications.
Yao
Sheng
S
Department of Chemistry and CREOL, College of Optics and Photonics, University of Central Florida, PO Box 162366, Orlando, Florida 32816-2366, USA.
Schafer-Hales
Katherine J
KJ
Belfield
Kevin D
KD
D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D011874:Radiometry; D012995:Solubility; D014867:Water
D005456:Fluorescent Dyes; D014867:Water