title: Repair mechanism of DNA-protein cross-link damage in Escherichia coli.
abstract: DNA-protein cross-link (DPC) damage is produced by varieties of genotoxic agents such as ionizing radiation, UV light, aldehydes, and some chemotherapeutic compounds. DPCs would be harmful to cells since proteins immobilized on DNA strands could hamper DNA transactions in cells. However, it has not been clarified how DPCs are repaired in cells. In the present study, we prepared DNA substrates containing defined DPCs and tested them for repair enzymes. We also examined the sensitivity of Escherichia coli to DPC-inducing agents.
authors.affiliation: Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nakano
authors.forename: Soh
authors.initials: S
authors.lastname: Morishita
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Terato
authors.forename: Seung Pil
authors.initials: SP
authors.lastname: Pack
authors.forename: Keisuke
authors.initials: K
authors.lastname: Makino
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ide
mesh_terms: D003432:Cross-Linking Reagents / Q000633:toxicity; D018736:DNA Adducts / Q000737:chemistry* / Q000378:metabolism; D004249:DNA Damage; D045647:DNA Glycosylases / Q000378:metabolism; D004260:DNA Repair; D045643:DNA Repair Enzymes / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005557:Formaldehyde / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D011684:Purine Nucleosides / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D018736:DNA Adducts; D011684:Purine Nucleosides; C468766:oxanine; D005557:Formaldehyde; D045647:DNA Glycosylases; C473634:NEIL1 protein, human; D045643:DNA Repair Enzymes