title: Diagnostic cellular organization features extracted from autofluorescence images.
abstract: Depth-resolved NADH autofluorescence images are shown to differentiate between normal and precancerous engineered tissues. An inverse power law behavior of the power spectral density (PSD) of these images is observed, indicating a self-affine organization of mitochondrial NADH at length scales 1-10 microm. Power exponents of the PSD functions vary significantly with tissue depth and precancerous state, giving insight into the morphological changes associated with precancerous lesions and providing substantial potential for noninvasive clinical diagnosis of squamous epithelial lesions and tumors.
authors.affiliation: Biomedical Engineering Department, Tufts University, Medford, Massachusetts 02155, USA. Jonathan.Levitt@tufts.edu
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Levitt
authors.forename: Martin
authors.initials: M
authors.lastname: Hunter
authors.forename: Claudia
authors.initials: C
authors.lastname: Mujat
authors.forename: Margaret
authors.initials: M
authors.lastname: McLaughlin-Drubin
authors.forename: Karl
authors.initials: K
authors.lastname: Münger
authors.forename: Irene
authors.initials: I
authors.lastname: Georgakoudi
mesh_terms: D017709:Fractals; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D021962:Membrane Microdomains / Q000473:pathology; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D008928:Mitochondria / Q000473:pathology*; D009243:NAD / Q000737:chemistry*; D009369:Neoplasms / Q000473:pathology*; D018307:Neoplasms, Squamous Cell / Q000473:pathology*; D055095:Optics and Photonics; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods
chemical_list: D009243:NAD