[The development and in vitro experiment study of a bio-type root canal filling sealer using calcium phosphate cement].
OBJECTIVE
The purpose of this study is to develop a novel root canal filling sealers based on calcium phosphate cement (CPC), and to evaluate its physical-chemical properties and in vitro antibacterial activity on the predominant bacteria infecting root canal.


METHODS
The fluidity and the setting time of the sealer were tested according to ISO 6876:2001(E) standards. The crystal size of the final product was determined. Its opacification with different composition were measured. The in vitro antibacterial property of the sealer was tested according to the Antimicrobial Susceptibility Testing of Anaerobes recommended by National Committee for Clinical Laboratory Standards (NCCLs). The involved bacteria included Actinomyces naeslundii(A. naeslundii), Peptostreptococcus anaerobius (P. anaerobius), Porphyromonas gingivalis (P. gingivalis), Porphyromonas endodpntalis (P. endodpntalis) and Fusobacterium nucleatum (F. nucleatum). Twenty single-rooted human extracted teeth were selected to evaluate the sealing ability using dye microleakage technology. Dye penetration was measured and the results were statistically analyzed using SPSS12.0 software package.


RESULTS
The new root canal filling sealer was primarily composed of hydroxyapatite in 279 nm after setting. Its liquidity was suitable, the operating time was over 30 minutes, and the controlled setting time was (1.0+/-0.5) hours. The opacification was acceptable. MIC<or=0.156-0.312. The dye penetration was significant different between two filling types (P<0.01).


CONCLUSIONS
The new root canal filling sealer is an ideal root canal filling sealer. Supported by Science and Technology Development Fund from the Science and Technology Bureau of Xiamen City (Grant No.2002-60-5).
Chen
Zuo-liang
ZL
Stomatological Hospital of Xiamen, Department of Stomatology, Fujian Medical University, Xiamen 361003, Fujian Province, China.
Wei
Wei
W
Feng
Zhu-de
ZD
Liu
Xue-qing
XQ
Chen
Xiao-ling
XL
Huang
Wen-xia
WX
D002130:Calcium Phosphates; D003738:Dental Cements; D003763:Dental Leakage; D003786:Dental Pulp Cavity; D005899:Glass Ionomer Cements; D006801:Humans; D066298:In Vitro Techniques; D016966:Porphyromonas gingivalis; D012387:Root Canal Filling Materials; D012389:Root Canal Obturation; D012390:Root Canal Therapy
D002130:Calcium Phosphates; D003738:Dental Cements; D005899:Glass Ionomer Cements; D012387:Root Canal Filling Materials; C020243:calcium phosphate