Preparation and metal ion-binding of 4-N-substituted cytosine pairs in DNA duplexes.
We report the synthesis and metal ion-binding properties of DNA duplexes containing 4-N-substituted cytosine base pairs. Thermal denaturation studies of these modified DNA duplexes in the presence of various metal ions revealed that the DNA duplexes containing 4-N-carboxymethylcytosine (1) base pair(s) bound Ag (I), Ni(II), and Cu(II) ions. Moreover, ESI-TOF MS analysis of the DNA duplexes containing 1-1 base pair(s) in the presence of Cu(II) ions was consistent with one 1-1 base pair region binding one equivalent of Cu(II) ions. These results indicate that the DNA duplexes containing 1-1 base pair(s) may be useful as a new metal ion-binding motif.
Sugiyama
Kumiko
K
Department of Material and Life Chemistry, Faculty of Engineering, Kanagawa University, 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama, Kanagawa 221-8686, Japan.
Kageyama
Yoshihiko
Y
Okamoto
Itaru
I
Ono
Akira
A
D020029:Base Pairing; D003300:Copper / Q000737:chemistry; D003596:Cytosine / Q000031:analogs & derivatives* / Q000737:chemistry; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D019216:Metals, Heavy / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D007477:Ions; D019216:Metals, Heavy; D009841:Oligonucleotides; D012834:Silver; D003300:Copper; D009532:Nickel; D003596:Cytosine; D004247:DNA