title: [Construction of tissue engineered gingiva with gingival epithelia and acellular dermal matrix].
abstract: OBJECTIVE
  To culture human gingival epithelia in vitro, and to construct the tissue engineered gingiva with the acellular dermal matrix (ADM).
  
  
  METHODS
  Human gingival epithelia were isolated from the gingival tissue, and the cells were cultured and seeded onto the surface of ADM. After 7 days of submerged incubation, an air-liquid interface culture was performed for 7, 14, and 21 days. The complex constructed above was taken for histological examination.
  
  
  RESULTS
  Human gingival epithelia could proliferate well on the surface of ADM, and form multilayer structure. But the superficial epithelium was partially keratinized.
  
  
  CONCLUSIONS
  Tissue engineered gingiva may be constructed with human gingival epithelia and ADM in vitro.
authors.affiliation: Department of Stomatology, Xiangya Hospital, Changsha 410008, China. cyfangdental@mail.csu.edu.cn.
authors.forename: Chang-yun
authors.initials: CY
authors.lastname: Fang
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Xie
authors.forename: Bin-jie
authors.initials: BJ
authors.lastname: Liu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Su
authors.forename: Hui-li
authors.initials: HL
authors.lastname: Li
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Shi
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Pang
authors.forename: Wei-ying
authors.initials: WY
authors.lastname: Zhong
mesh_terms: D062088:Acellular Dermis; D002478:Cells, Cultured; D003238:Connective Tissue; D004847:Epithelial Cells / Q000166:cytology*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods*