title: Positive Streptobacillus moniliformis PCR in guinea pigs likely due to Leptotrichia spp.
abstract: Streptobacillus moniliformis is a zoonotic bacterium. We obtained positive S. moniliformis PCR results in oral swab samples from guinea pigs from an experimental colony and the breeding colony of origin. Comparison of the DNA sequence of an amplicon with deposited 16S rDNA sequences revealed that Leptotrichia sp. can be the source of a false positive S. moniliformis PCR outcome.
authors.affiliation: Section of Laboratory Animal Microbiology, Diagnostic Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands. r.boot@rivm.nl
authors.forename: Ron
authors.initials: R
authors.lastname: Boot
authors.forename: Lia
authors.initials: L
authors.lastname: Van de Berg
authors.forename: Frans A G
authors.initials: FA
authors.lastname: Reubsaet
authors.forename: Maurice J
authors.initials: MJ
authors.lastname: Vlemminx
mesh_terms: D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005189:False Positive Reactions; D005674:Fusobacterium Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D006168:Guinea Pigs / Q000382:microbiology*; D045183:Leptotrichia / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards / Q000662:veterinary*; D012376:Rodent Diseases / Q000175:diagnosis* / Q000382:microbiology; D016415:Sequence Alignment / Q000662:veterinary; D013045:Species Specificity; D013288:Streptobacillus / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal