[Effect of branched-chain amino acid-rich parenteral nutrition on protein metabolism after partial hepatectomy in rats with liver cirrhosis].
OBJECTIVE
To observe the effect of parenteral nutrition (PN) with branched-chain amino acid supplementation on protein metabolism after partial hepatectomy in rats with liver cirrhosis.


METHODS
Eighteen rats with liver cirrhosis were randomly divided into pre-operation group (n=6), post-operation 8.5% Novamin PN group (n=6) and post-operation 10% Hepa PN group (n=6), with 6 normal rats severing as the normal control group. Five days after the operation, serum albumin (ALB), insulin-like growth factor I (IGF-1) and plasma amino acid spectrum were measured, and ALB mRNA level in the liver was assayed using RT-PCR.


RESULTS
Postoperative serum ALB was similar between 10% Hepa PN and 8.5% Novamine PN groups, but the rats in the latter group showed significantly increased serum IGF-1 level, Fischer ratio and hepatic ALB mRNA expression (P<0.05).


CONCLUSIONS
Administration of PN with branched-chain amino acid supplementation can ameliorate plasma amino acid spectrum and increase protein synthesis in rats with liver cirrhosis after partial hepatectomy.
Cao
Jie
J
Department of General Surgery, First People's Hospital of Guangzhou Affiliated to Guangzhou Medical College, Guangzhou 510180, China.
Luo
Shi-min
SM
Liang
Li-jian
LJ
Hu
Wen-jie
WJ
D000418:Albumins / Q000378:metabolism*; D000597:Amino Acids, Branched-Chain / Q000008:administration & dosage / Q000627:therapeutic use*; D000818:Animals; D006498:Hepatectomy; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000628:therapy*; D010288:Parenteral Nutrition; D051381:Rats; D017207:Rats, Sprague-Dawley
D000418:Albumins; D000597:Amino Acids, Branched-Chain; D007334:Insulin-Like Growth Factor I