title: Expression of cell cycle regulator p16 is not affected by diabetes during oral oncogenesis.
abstract: BACKGROUND
  The tumor suppressor protein p16 plays a vital role in the regulation of the cell cycle. The expression of p16 was investigated in an experimental model of chemically induced carcinogenesis in normal and diabetic (type I) Sprague-Dawley rats.
  
  
  METHODS
  Tissue sections ranging from normal oral mucosa to moderately differentiated oral squamous cell carcinoma (OSCC) were studied immunohistochemically.
  
  
  RESULTS
  In normal rats p16 expression increased gradually during oral oncogenesis, but a significant increase was observed only in moderately differentiated OSCC (p=0.038). On the contrary, in diabetic rats the detected gradual increase was significant in hyperplasia, dysplasia, early invasion and well-differentiated OSCC (p<0.001). Nevertheless, there was no significant difference in p16 expression during oral oncogenesis between normal and diabetic animals.
  
  
  CONCLUSIONS
  It seems that the expression of cell cycle regulator p16 is not affected by diabetes in the studied animal model of oral oncogenesis.
authors.affiliation: Department of Oral and Maxillofacial Surgery, University of Athens Medical School, Athens 11521, Greece. lvairakt@med.uoa.gr
authors.forename: Eleftherios
authors.initials: E
authors.lastname: Vairaktaris
authors.forename: Lambros
authors.initials: L
authors.lastname: Goutzanis
authors.forename: Emeka
authors.initials: E
authors.lastname: Nkenke
authors.forename: Sofia
authors.initials: S
authors.lastname: Spyridonidou
authors.forename: Stavros
authors.initials: S
authors.lastname: Vassiliou
authors.forename: Spyridoula
authors.initials: S
authors.lastname: Derka
authors.forename: Antonis
authors.initials: A
authors.lastname: Vylliotis
authors.forename: Christos
authors.initials: C
authors.lastname: Yapijakis
authors.forename: Andreas
authors.initials: A
authors.lastname: Lazaris
authors.forename: Efstratios
authors.initials: E
authors.lastname: Patsouris
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D003920:Diabetes Mellitus / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology*; D009361:Neoplasm Invasiveness; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16