title: Binding effect of the antitumor active dirhodium (II) compounds to plasmid DNA.
abstract: Binding effect of the antitumor dirhodium (II) compounds to the plasmid pUC19 DNA has been studied under different molar ratio of Rh (II) compounds to base pair of pUC19 DNA (Rf) and time. The electrophoresis results indicate that Rh binding affect the DNA conformation and therefore, Rh-DNA migrated in the different position comparing with native DNA. At high value of Rf ICP-MS (Inductively Coupled Plasma Mass Spectrometry) measurement confirmed that 46% of Rh binds to DNA. In vitro result shows that DNA synthesis was inhibited by the Rh binding.
authors.affiliation: Department of Ecological Engineering, Toyohashi University of Technology, Tempaku-cho, Toyohashi, Aichi, 441-8580, Japan.
authors.forename: Md Masudur
authors.initials: MM
authors.lastname: Rahman
authors.forename: Hachiro
authors.initials: H
authors.lastname: Yasuda
authors.forename: Kazunori
authors.initials: K
authors.lastname: Takashima
authors.forename: Akira
authors.initials: A
authors.lastname: Mizuno
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D009942:Organometallic Compounds / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry*; D012238:Rhodium / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D009942:Organometallic Compounds; D004247:DNA; D012238:Rhodium