title: Intestinal STC-1 cells respond to five basic taste stimuli.
abstract: STC-1 cells have been established as an enteroendocrine cell line from the small intestine. By monitoring the level of intracellular Ca using a calcium-imaging technique, cellular responses of intestinal STC-1 cells to compounds of five basic tastants were investigated. Although this cell line was known to respond to bitter compounds, we found that compounds of the other four basic tastants also stimulate STC-1 cells. When solutions containing glutamate, sucrose, HCl, or NaCl were applied, the intracellular Ca concentration in STC-1 cells significantly increased. We thus demonstrated that the gastrointestinal system can sense all five basic taste stimuli, and that STC-1 cells emerge as a new cell model for studying the molecular mechanism of signaling pathways of the five basic tastants.
authors.affiliation: Department of Bio-Science, Faculty of Bio-Science, Nagahama Institute of Bio-Science and Technology, Nagahama-shi, Shiga, Japan. o_saito@nagahama-i-bio.ac.jp
authors.forename: Osamu
authors.initials: O
authors.lastname: Saitoh
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hirano
authors.forename: Yui
authors.initials: Y
authors.lastname: Nishimura
mesh_terms: D000814:Aniline Compounds; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D019858:Enteroendocrine Cells / Q000187:drug effects / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D006851:Hydrochloric Acid / Q000378:metabolism / Q000494:pharmacology; D007202:Indicators and Reagents; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology; D008954:Models, Biological; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D012965:Sodium Chloride / Q000378:metabolism / Q000494:pharmacology; D013395:Sucrose / Q000378:metabolism / Q000494:pharmacology; D013649:Taste / Q000187:drug effects / Q000502:physiology*; D013650:Taste Buds / Q000502:physiology; D014966:Xanthenes
chemical_list: D000814:Aniline Compounds; C409648:Fluo 4; D007202:Indicators and Reagents; D014966:Xanthenes; D018698:Glutamic Acid; D012965:Sodium Chloride; D013395:Sucrose; D006851:Hydrochloric Acid; D002118:Calcium