[Phospho-FACS: a powerful tool for exploring intracellular transduction cascades].
BACKGROUND
FACS (fluorescence-activated cell sorting), or flow cytometry, was developed in 1971 by Leonard Herzenberg's team at Stanford University. Under continuous development, this technology enables single-cell multiparametric analysis and sorting, based on physical properties of cells and/or their relative expression levels of specific glycoproteic epitopes and metabolites.


BACKGROUND
Recently, the use of fluorescent antibodies specific for phosphorylated epitopes - or "phospho-epitopes" - within proteins of interest has further extended the range of FACS analyses. This new application, dubbed "phospho-FACS", has quickly become a tool of choice for delineating intracellular phosphorylation cascades.


CONCLUSIONS
In both basic research and clinical research, the application of phospho-FACS to cellular subsets from blood or the periphery, whether frequent or rare, enables the discovery of pathological biomarkers and therapeutic innovation.


CONCLUSIONS
Thanks to its rapid implementation and its ability to generate single-cell data, the phospho-FACS technique features numerous advantages compared to preexisting analytical methods for intracellular phosphorylation cascades.
Gernez
Y
Y
Département de Génétique, Université de Stanford, Stanford, Californie, Etats-Unis d'Amérique.
Herzenberg
L A
LA
Herzenberg
L A
LA
Tirouvanziam
R
R
D000906:Antibodies / Q000032:analysis; D002470:Cell Survival; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D010750:Phosphoproteins / Q000276:immunology; D010766:Phosphorylation; D015398:Signal Transduction
D000906:Antibodies; D000939:Epitopes; D010750:Phosphoproteins