title: Immobilization of fibronectin onto a large area nanoimprinted polystyrene patterns.
abstract: A well known fibrous protein, fibronectin was successfully immobilized onto well-fabricated nanopatterns of polystyrene (PS) substrates. The nanopatterns with finely-defined physical dimensions were obtained by nanoimprinting lithography (NIL) using anodized aluminum oxide (AAO) molds of which the pore sizes were controlled in nanometer scale; 45 nm, 250 nm and 410 nm for the widths and 110 nm and 500 nm for the pitches. Discrete changes of the surface images before and after the protein-adsorption on the patterns were demonstrated by scanning electron microscope (SEM), and the effect of the patterned features on the immobilization has been investigated and discussed.
authors.affiliation: Department of Biomedical Engineering, College of Medicine, Korea University, Seoul 136-705, Korea. nalarichem.lee@yahoo.co.kr
authors.forename: Wonbae
authors.initials: W
authors.lastname: Lee
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Ko
authors.forename: Kyu Back
authors.initials: KB
authors.lastname: Lee
mesh_terms: D001665:Binding Sites; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003460:Crystallization / Q000379:methods*; D005353:Fibronectins / Q000737:chemistry* / Q000648:ultrastructure*; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry*; D011485:Protein Binding; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D011137:Polystyrenes