Electric impedance assisted micropipette aspiration.
Micropipette aspiration is a technique to selectively isolate cells from cell cultures using suction pressure. Cells can selectively be isolated one by one from neighboring cells into the micropipette. This paper presents a novel micropipette aspiration system assisted by an impedance measurement system. Furthermore, a method to reduce the adhesion force at a single cell level for a gentler detachment of the cell from a cultivation surface and surrounding cell connections is proposed.
Kovanen
Katrin
K
Institute of Automation and Control, Tampere University of Technology, 33101 Tampere, Finland.
Lukkari
Mikko
M
Purmonen
Sami
S
Ylikomi
Timo
T
Viitanen
Jouko
J
Kallio
Pasi J
PJ
D002468:Cell Physiological Phenomena; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured / Q000166:cytology* / Q000502:physiology*; D017097:Electric Impedance; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D008846:Micromanipulation / Q000379:methods*; D013396:Suction / Q000379:methods*