title: [Determination of tenuifolin in Tianwang Buxinwan and Guipiwan by HPLC].
abstract: OBJECTIVE
  A quantitative method was developed for the determination of tenuifolin in Tianwang Buxinwan and Guipiwan by HPLC.
  
  
  METHODS
  The samples were separated by Alltima C18 column (4.6 mm x 250 mm, 5 microm) using methanol--0.05% phosphoric acid (65:35) as a mobile phase, flow rate was 1.0 mL x min(-1) and wavelength was set at 202 nm.
  
  
  RESULTS
  Tenuifolin was detected in both Chinese preparations. The number of theoretical plates calculated by tenuifolin peak was 2 500. The regression equation of tenuifolin was Y = 5.239 x 10(6) X-6.247 x 10(5) (r = 0.9994) and the liner range was 10-500 g x mL(-1). The average recovery of tenuifolin was 97.5% (RSD less than 3.0%). The LOD of tenuifolin was 5.50 g x mL(-1).
  
  
  CONCLUSIONS
  The method is sensitive, rapid and accurate.
authors.affiliation: Institute of Chinese Medical Sciences of Macau University, Macau, China. dxbaa@ust.hk
authors.forename: Xiao-bing
authors.initials: XB
authors.lastname: Dong
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.forename: T X Tina
authors.initials: TX
authors.lastname: Dong
authors.forename: Peng-fei
authors.initials: PF
authors.lastname: Tu
authors.forename: W K Karl
authors.initials: WK
authors.lastname: Tsim
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D029745:Codonopsis / Q000737:chemistry; D045786:Diterpenes, Kaurane / Q000032:analysis; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D031801:Polygala / Q000737:chemistry; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry; D012503:Saponins / Q000032:analysis; D014315:Triterpenes / Q000032:analysis
chemical_list: D045786:Diterpenes, Kaurane; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C532372:guipiwan; D012503:Saponins; C532370:tenuifolin diterpene; C532371:tianwang buxinwan; D014315:Triterpenes; C407311:tenuifolic saponin