Novel analysis for single nucleotide polymorphism using cationic comb-type copolymers.
We have focused on the DNA strand exchange reaction (SER) as a format of a single-base mismatch detection. We employed a partially double-stranded (PDS) DNA probe having a single-stranded (ss) portion. We could rapidly recognize a single-base mismatch by the SER rates between the PDS probes and target ss DNAs. In the presence of cationic comb-type copolymers (PLL-g-Dex) and betaine, the PDS probes could clearly discriminate between A:T matched and A:G mismatched targets. Furthermore, the PDS probes could discriminate among homo- and hetero-type alleles.
Shimada
Naohiko
N
Institute for Materials Chemistry and Engineering, Kyushu University, Motooka 744, Nishi-ku, Fukuoka 819-8581, Japan and.
Muraki
Kentaro
K
Anai
Taro
T
Kano
Arihiro
A
Maruyama
Atsushi
A
D020137:Base Pair Mismatch; D004252:DNA Mutational Analysis / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D011107:Polylysine / Q000031:analogs & derivatives* / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide
D015342:DNA Probes; D003911:Dextrans; C113090:poly(lysine)-graft-dextran; D011107:Polylysine