Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis.
A cDNA library based on mRNA from adult worms of Echinococcus multilocularis was constructed. One cDNA clone, emY162, was isolated from this cDNA library. The putative protein from emY162 cDNA consists of 153 amino acids and has a predicted molecular weight of 17.0 kDa. The amino acid sequences of EMY162 are predicted to be a hydrophobic N-terminus conserving a secretory signal, and a hydrophobic C-terminus encoding a transmembrane domain or glycosyl-phosphatylinositol membrane anchor, and to have single fibronectin type III-like domain. In addition, it was shown that the emY162 gene (1738 bp) in the E. multilocularis genome DNA consists of three exons and two introns, and that emY162 is expressed in all four stages (protoscoleces, cultured metacestodes, immature adult worms and mature adult worms). Moreover, immunity to recombinant EMY162, which comprises the fibronectin type III-like domain on the EMY162 protein, was examined. Immune responses to the recombinant EMY162 were studied by using serum from dogs infected with E. multilocularis. Strong IgG immune responses were detected in Western blots.
Katoh
Yoshinobu
Y
Department of Biological Science, Center for Infectious Disease Prevention, Hokkaido Institute of Public Health, Sapporo, Hokkaido 060-0819, Japan. kyoshi@iph.pref.hokkaido.jp
Kouguchi
Hirokazu
H
Matsumoto
Jun
J
Goto
Akiko
A
Suzuki
Tomohiro
T
Oku
Yuzaburo
Y
Yagi
Kinpei
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D004285:Dogs; D048210:Echinococcus multilocularis / Q000235:genetics* / Q000378:metabolism; D015723:Gene Library; D015801:Helminth Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D015801:Helminth Proteins; D007106:Immune Sera; D007074:Immunoglobulin G; D011994:Recombinant Proteins