In vitro- and in vivo-induced transgene expression in human embryonic stem cells and derivatives.
The use of human embryonic stem cells (hESCs) as a research and therapeutic tool will be facilitated by conditional gene expression. Here, we report drug-induced transgene expression, both in vitro and in vivo, from a tet-on hESC line with >95% purity. Using green fluorescent protein as an indicator, we demonstrated that the tet-on system allowed a tight control of the gene expression in both undifferentiated hESCs and differentiated cells of the three germ layers. More importantly, after the cells were transplanted into animals, the gene expression remained to be regulated by an orally administered drug. These results provide a technical basis for regulation of gene expression in hESCs and derivatives in vitro and in vivo.
Xia
Xiaofeng
X
WiCell Research Institute, Madison, Wisconsin, USA.
Ayala
Melvin
M
Thiede
Benjamin R
BR
Zhang
Su-Chun
SC
D000284:Administration, Oral; D000818:Animals; D016380:Brain Tissue Transplantation; D002454:Cell Differentiation; D002460:Cell Line; D004318:Doxycycline / Q000008:administration & dosage / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000637:transplantation; D015870:Gene Expression / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000096:biosynthesis; D051379:Mice; D016513:Mice, SCID; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics; D013724:Teratoma / Q000209:etiology; D013752:Tetracycline / Q000494:pharmacology; D014162:Transfection
D007328:Insulin; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D013752:Tetracycline; D004318:Doxycycline