High-resolution MEA platform for in-vitro electrogenic cell networks imaging.
A platform based on an active-pixel-sensor electrode array (APS-MEA) for high-resolution imaging of in-vitro electrogenic cell cultures is presented, characterized and validated under culture conditions. The system enables full frame acquisition at 8 kHz from 4096 microelectrodes integrated with separations of 21 microm and zoomed area acquisition with temporal resolutions down to 8 micros. This bi-modal acquisition feature opens new perspectives in particular for neuronal activity analysis and for the correlation of micro-scale and macro-scale behaviors. The low-noise performances of the integrated amplifier (11 microVRMS) combined with a hardware implementation reflecting image-/video-concepts enable high-resolution acquisitions with real-time processing capabilities adapted to the handling of the large amount of acquired data.
Imfeld
K
K
Electronics and Signal Processing Laboratory, Institute of Microtechnology University of Neuchâtel, Rue A.-L. Breguet 2, CH-2000 Neuchâtel, Switzerland. Kilian.Imfeld@unine.ch
Garenne
A
A
Neukom
S
S
Maccione
A
A
Martinoia
S
S
Koudelka-Hep
M
M
Berdondini
L
L
D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D003952:Diagnostic Imaging / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation* / Q000379:methods; D008839:Microelectrodes; D009415:Nerve Net / Q000502:physiology*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*