title: Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure.
abstract: The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80 degrees C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.
authors.affiliation: Ghent University, Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation, Coupure links 653, 9000 Ghent, Belgium.
authors.forename: Leen
authors.initials: L
authors.lastname: Baert
authors.forename: Christiane E
authors.initials: CE
authors.lastname: Wobus
authors.forename: Els
authors.initials: E
authors.lastname: Van Coillie
authors.forename: Larissa B
authors.initials: LB
authors.lastname: Thackray
authors.forename: Johan
authors.initials: J
authors.lastname: Debevere
authors.forename: Mieke
authors.initials: M
authors.lastname: Uyttendaele
mesh_terms: D000818:Animals; D017250:Caliciviridae Infections / Q000821:virology; D005759:Gastroenteritis / Q000821:virology; D006358:Hot Temperature; D051379:Mice; D029322:Norovirus / Q000235:genetics* / Q000254:growth & development; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013696:Temperature; D014162:Transfection / Q000379:methods*; D010948:Viral Plaque Assay / Q000379:methods*
chemical_list: D012367:RNA, Viral