title: Enhanced tolerance of transgenic potato plants overexpressing nucleoside diphosphate kinase 2 against multiple environmental stresses.
abstract: In plants, nucleoside diphosphate kinase 2 (NDPK2) is known to regulate the expression of antioxidant genes. In this study, we developed transgenic potato plants (Solanum tuberosum L. cv. Atlantic) expressing Arabidopsis NDPK2 (AtNDPK2) gene in cytosols under the control of an oxidative stress-inducible SWPA2 promoter (referred to as SN plants) or enhanced CaMV 35S promoter (EN plants) and evaluated their tolerance to various environmental stress, including methyl viologen (MV)-mediated oxidative stress, high temperature, and salt stress. When 250 muM MV was sprayed to whole plants, plants expressing NDPK2 showed significantly an enhanced tolerance compared to non-transgenic (NT) plants. SN plants and EN plants showed 51% and 32% less visible damage than NT plants, respectively. Transcript level of AtNDPK2 gene and NDPK2 activity in SN plants following MV treatment well reflected the plant phenotype. Ascorbate peroxidase (APX) activity was also increased in MV-treated SN plants. In addition, SN plants showed enhanced tolerance to high temperature at 42 degrees C. The photosynthetic activity of SN plants after treatment of high temperature was decreased by about 10% compared to the plants grown at 25 degrees C, whereas that of NT plants declined by 30%. When treated with 80 mM NaCl onto the plantlets, both SN plants and EN plants also showed a significant reduced damage in root growth. These results indicate that overexpression of NDPK2 under the stress-inducible SWPA2 promoter might efficiently regulate the oxidative stress derived from various environmental stresses.
authors.affiliation: Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon, Korea.
authors.forename: Li
authors.initials: L
authors.lastname: Tang
authors.forename: Myoung Duck
authors.initials: MD
authors.lastname: Kim
authors.forename: Kyoung-Sil
authors.initials: KS
authors.lastname: Yang
authors.forename: Suk-Yoon
authors.initials: SY
authors.lastname: Kwon
authors.forename: Sun-Hyung
authors.initials: SH
authors.lastname: Kim
authors.forename: Jin-Seog
authors.initials: JS
authors.lastname: Kim
authors.forename: Dae-Jin
authors.initials: DJ
authors.lastname: Yun
authors.forename: Sang-Soo
authors.initials: SS
authors.lastname: Kwak
authors.forename: Haeng-Soon
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D017360:Arabidopsis / Q000201:enzymology; D060387:Ascorbate Peroxidases; D018506:Gene Expression Regulation, Plant; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D010269:Paraquat / Q000494:pharmacology; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012965:Sodium Chloride / Q000494:pharmacology*; D011198:Solanum tuberosum / Q000235:genetics* / Q000378:metabolism; D013696:Temperature
chemical_list: D012333:RNA, Messenger; D018749:RNA, Plant; D012965:Sodium Chloride; D010544:Peroxidases; D060387:Ascorbate Peroxidases; C121988:nucleoside diphosphate kinase 2; D009701:Nucleoside-Diphosphate Kinase; D010269:Paraquat