title: Regulation of cAMP on the first mitotic cell cycle of mouse embryos.
abstract: Mitosis promoting factor (MPF) plays a central role during the first mitosis of mouse embryo. We demonstrated that MPF activity increased when one-cell stage mouse embryo initiated G2/M transition following the decrease of cyclic adenosine 3', 5'-monophosphate (cAMP) and cAMP-dependent protein kinase (PKA) activity. When cAMP and PKA activity increases again, MPF activity decreases and mouse embryo starts metaphase-anaphase transition. In the downstream of cAMP/PKA, there are some effectors such as polo-like kinase 1 (Plk1), Cdc25, Mos (mitogen-activated protein kinase kinase kinase), MEK (mitogen-activated protein kinase kinase), mitogen-activated protein kinase (MAPK), Wee1, anaphase-promoting complex (APC), and phosphoprotein phosphatase that are involved in the regulation of MPF activity. Here, we demonstrated that following activation of MPF, MAPK activity was steady, whereas Plk1 activity fluctuated during the first cell cycle. Plk1 activity was the highest at metaphase and decreased at metaphase-anaphase transition. Further, we established a mathematical model using Gepasi algorithm and the simulation was in agreement with the experimental data. Above all the evidences, we suggested that cAMP and PKA might be the upstream factors which were included in the regulation of the first cell cycle development of mouse embryo.
authors.affiliation: Department of Biochemical and Molecular Biology, China Medical University, Shenyang, Liaoning, China.
authors.forename: Aiming
authors.initials: A
authors.lastname: Yu
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Bi
authors.forename: Bingqi
authors.initials: B
authors.lastname: Sun
authors.forename: Wenhui
authors.initials: W
authors.lastname: Su
authors.forename: Yifu
authors.initials: Y
authors.lastname: Guan
authors.forename: Runqing
authors.initials: R
authors.lastname: Mu
authors.forename: Changsheng
authors.initials: C
authors.lastname: Miao
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Bingzhi
authors.initials: B
authors.lastname: Yu
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D000090204:Mesothelin; D051379:Mice; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D008938:Mitosis / Q000502:physiology*; D011247:Pregnancy; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D000097869:Polo-Like Kinase 1
chemical_list: D018797:Cell Cycle Proteins; D000242:Cyclic AMP; D000090204:Mesothelin; D020929:Mitogen-Activated Protein Kinase Kinases; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1; C000715528:Msln protein, mouse; C102333:histone H1 kinase