Different effects of nanophase and conventional hydroxyapatite thin films on attachment, proliferation and osteogenic differentiation of bone marrow derived mesenchymal stem cells.
It is suggested that nanophase hydroxyapatite (nHAP) might have advantages over conventional hydroxyapatite (cHAP) as a biomaterial for bone regeneration. To be a satisfactory candidate for bone tissue engineering, it is important to support the growth and differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). The purpose of this study is to determine whether nHAP as cell growth substrata could give better support for attachment, proliferation and osteogenic differentiation of BMSCs than cHAP.


METHODS
nHAP and cHAP films were prepared as the substrata for the cell growth. BMSCs obtained from rabbit were seeded on the films. Attachment, proliferation and osteogenic differentiation of BMSCs on the two kinds of films were evaluated.


RESULTS
Cell attachment ratio on nHAP films was significantly higher than that on cHAP films. Doubling time on nHAP films was significantly shorter than that on cHAP films. Amount of total proteins detected from cells cultured on nHAP films was significantly higher than that on cHAP films. However, alkaline phosphatase activity and osteocalcin content of the two groups showed no significant difference.


CONCLUSIONS
nHAP films favored cell attachment and proliferation but not osteogenic differentiation of BMSCs compared with cHAP films.
Zhou
Guo Shun
GS
Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou, Zhejiang Province, China. gfz@mail.huptt.zj.cn
Su
Zhong Yuan
ZY
Cai
Yu Rong
YR
Liu
Yu Kan
YK
Dai
Li Cheng
LC
Tang
Rui Kang
RK
Zhang
Ming
M
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D002448:Cell Adhesion; D002452:Cell Count; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D017886:Durapatite; D048091:Guided Tissue Regeneration / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D053758:Nanoparticles; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis; D010316:Particle Size; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D015675:Osteocalcin; D017886:Durapatite; D000469:Alkaline Phosphatase