In vitro selection of a 5'-purine nucleotide transferase ribozyme.
Here we report a ribozyme that catalyzes the 5'-nucleotidyl transfer reaction forming the 2'-5' phosphodiester bond. This ribozyme was retrieved as a sole sequence in the pool enriched for the 5'-triphosphate-dependent activities in incorporating ATP-gammaS. The originally selected ribozyme consisting of 109-nucleotide was miniaturized to 45-nucleotide M4 ribozyme via mutation studies, and based on this mini-ribozyme a trans-acting system was constructed. Interestingly, M4 ribozyme promiscuously accepts a variety of purine nucleotides. This remarkable ability of M4 ribozyme would lead us to the development of a new tool for the 5'-modification of RNAs with unique chemical groups.
Kang
Taek Jin
TJ
Research Center for Advanced Science and Technology, The University of Tokyo, 153-8904 Tokyo, Japan.
Suga
Hiroaki
H
D001483:Base Sequence; D019020:Directed Molecular Evolution; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009713:Nucleotidyltransferases / Q000737:chemistry* / Q000378:metabolism; D011122:Polyphosphates / Q000378:metabolism; D011685:Purine Nucleotides / Q000378:metabolism*; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity
D011122:Polyphosphates; D011685:Purine Nucleotides; D016337:RNA, Catalytic; D009713:Nucleotidyltransferases