Correlating enzyme activity and cellular behavior.
A photoactivatable phosphorylation sensor used in conjunction with isoform specific inhibitors of protein kinase C enables the enzyme's activity to be monitored inside cells at specific time points during mitosis. PKCbeta was found to be active before, but not after, nuclear envelope breakdown.
Dore
Timothy M
TM
Department of Chemistry, University of Georgia, Athens, GA 30602-2556, USA. tdore@chem.uga.edu
D031541:Fluorescence Resonance Energy Transfer; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*
D011493:Protein Kinase C