Submicrometer axial resolution optical coherence tomography.
Optical coherence tomography (OCT) with unprecedented submicrometer axial resolution achieved by use of a photonic crystal fiber in combination with a compact sub-10-fs Ti:sapphire laser (Femtolasers Produktions) is demonstrated for what the authors believe is the first time. The emission spectrum ranges from 550 to 950 nm (lambda(c)=725 nm , P(out)=27 mW) , resulting in a free-space axial OCT resolution of ~0.75 mum , corresponding to ~0.5 mum in biological tissue. Submicrometer-resolution OCT is demonstrated in vitro on human colorectal adenocarcinoma cells HT-29. This novel light source has great potential for development of spectroscopic OCT because its spectrum covers the absorption bands of several biological chromophores.
Povazay
B
B
Bizheva
K
K
Unterhuber
A
A
Hermann
B
B
Sattmann
H
H
Fercher
A F
AF
Drexler
W
W
Apolonski
A
A
Wadsworth
W J
WJ
Knight
J C
JC
Russell
P St J
PS
Vetterlein
M
M
Scherzer
E
E