[Separation of 1,4-benzodiazepine derivates by micellar elektrokinetic capillary chromatography].
Our aim was to develop a capillary electrophoresis method for the separation of 1,4-benzodiazepine derivates. Benzodiazepines are hydrophobic substances, neutral from electrophoretic point of view, consequently the best method for their separation proved to be micellar elektrokinetic capillary chromatography (MECC). A fast and reliable method has been developed, for the 8 of the most frequently used benzodiazepine derivates, using a separation buffer composed of sodium tetraborate 25 mM (pH 9.3), SDS (50 mM) and methanol (at least 12%) as an organic modifier. Beside the separation we also studied the analytical condition for the separation (i.e. the effects of buffer concentration, modifier concentration and buffer pH on the separation).
Hancu
Gabriel
G
Universiatatea de Medicină si Farmacie, Orvosi es Gyógyszerészeti Egyetem, Gyógyszerészeti Kémiai Tanszék, 540139 Târgu Mureş (Marosvásárhely), Gh. Marinescu u. 38, Románia. g_hancu@zappmobile.ro
Gyéresi
Arpád
A
Gáspár
Attila
A
D001569:Benzodiazepines / Q000737:chemistry / Q000302:isolation & purification*; D002021:Buffers; D053801:Capillary Electrochromatography / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008823:Micelles; D008958:Models, Molecular; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002021:Buffers; D007202:Indicators and Reagents; D008823:Micelles; D001569:Benzodiazepines; C468549:Bz-423