Synthesis of naphthyridine-conjugated DNA probes and their application to SNPs typing.
We have developed diaminonaphthyridine-conjugated DNA probes (DANP-ODNs), which contain diaminonaphtyridine (DANP) derivatives at 5' end of oligodeoxynucleotides. We here report the synthesis and fluorescence properties of aeDNAP-ODN, ahDANP-ODN, and baeDANP-ODN which contains N-amino ethyl-2,7-diamino-1,8-naphtyridine, N-aminohexyl-2,7-diamino1,8-naphthyridine, and N,N'-bisaminoethyl-2,7-diamino-1,8-naphtyridine, respectively. The emission spectra of DANP-ODNs were measured in the presence of their complementary strand having single nucleotide alteration. The fluorescence intensity at 420 nm of baeDANP-ODN is 12-fold higher in the presence of Callele ODN than that in the presence of A-allele ODN.
Kobori
Akio
A
Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology, Gosyokaidocho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Mori
Takashi
T
Yamayoshi
Asako
A
Murakami
Akira
A
D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009287:Naphthyridines / Q000138:chemical synthesis / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D013050:Spectrometry, Fluorescence
D015342:DNA Probes; D005456:Fluorescent Dyes; D009287:Naphthyridines; D009838:Oligodeoxyribonucleotides