title: Single nucleotide polymorphism mapping array assay.
abstract: Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation present in the human genome, and millions of SNPs have been identified http://www.ncbi.nlm.nih.gov/SNP/). Because of their abundance, even spacing, and stability across the genome, SNPs have significant advantages over other genetic markers (such as restriction fragment length polymorphisms and microsatellite markers) as a basis for high-resolution whole genome allelotyping. SNP scoring is easily automated and high-density oligonucleotide arrays have recently been generated to support large-scale high throughput SNP analysis. High-density SNP allele arrays have improved significantly and it is now possible to genotype hundreds of thousands SNP markers using a single SNP array. In this chapter, we will provide a detailed experimental protocol of Affymetrix GeneChip SNP Mapping Array-based whole genome SNP genotyping assay.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhou
authors.forename: David T W
authors.initials: DT
authors.lastname: Wong
mesh_terms: D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D015894:Genome, Human; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: D017931:DNA Primers