title: Evolutionary characterization of influenza virus A/duck/Hubei/W1/2004 (H9N2) isolated from central China.
abstract: Full-length eight gene segments of avian influenza virus A/duck/Hubei/W1/2004(H9N2) (Dk/Hub/W1/04) were amplified by RT-PCR and completely sequenced. Phylogenetic analysis revealed that Dk/Hub/W1/04 was derived from A/Duck/HongKong/Y280/97, not displaying direct evolutional relationship with A/Quail/HongKong/G1/97 or Hubei H5N1 viruses. Meanwhile, Dk/Hub/W1/04 was found highly related to recent three chicken isolates. The connecting peptide of HA and the deletion in NA stalk were consistent with three chicken isolates, and the number of potential glycosylation site on the HA and NA also was similar or identical to the three chicken isolates. These findings suggested that Dk/Hub/W1/04 is likely to transmit back to ducks from chickens.
authors.affiliation: Division of Animal Infectious Diseases, National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Xu
authors.forename: Gao-Yuan
authors.initials: GY
authors.lastname: Xu
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Zhou
authors.forename: Zheng-Jun
authors.initials: ZJ
authors.lastname: Yu
authors.forename: An-Ding
authors.initials: AD
authors.lastname: Zhang
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Song
authors.forename: Mei-Lin
authors.initials: ML
authors.lastname: Jin
authors.forename: Huan-Chun
authors.initials: HC
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001717:Birds / Q000821:virology; D002645:Chickens / Q000821:virology; D002681:China / Q000453:epidemiology; D004372:Ducks / Q000821:virology; D019143:Evolution, Molecular; D005814:Genes, Viral; D016679:Genome, Viral; D006031:Glycosylation; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000235:genetics*; D053127:Influenza A Virus, H9N2 Subtype / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005585:Influenza in Birds / Q000453:epidemiology* / Q000821:virology*; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D009439:Neuraminidase