title: The A5 gene of alcelaphine herpesvirus 1 encodes a constitutively active G-protein-coupled receptor that is non-essential for the induction of malignant catarrhal fever in rabbits.
abstract: Many gammaherpesviruses encode G-protein-coupled receptors (GPCRs). Several in vivo studies have revealed that gammaherpesvirus GPCRs are important for viral replication and for virus-induced pathogenesis. The gammaherpesvirus alcelaphine herpesvirus 1 (AlHV-1) is carried asymptomatically by wildebeest, but causes malignant catarrhal fever (MCF) following cross-species transmission to a variety of susceptible species. The A5 ORF of the AlHV-1 genome encodes a putative GPCR. In the present study, we investigated whether A5 encodes a functional GPCR and addressed its role in viral replication and in the pathogenesis of MCF. In silico analysis supported the hypothesis that A5 could encode a functional GPCR as its expression product contained several hallmark features of GPCRs. Expression of A5 as tagged proteins in various cell lines revealed that A5 localizes in cell membranes, including the plasma membrane. Using [35S]GTPgammaS and reporter gene assays, we found that A5 is able to constitutively couple to alpha i-type G-proteins in transfected cells, and that this interaction is able to inhibit forskolin-triggered cAMP response element-binding protein (CREB) activation. Finally, using an AlHV-1 BAC clone, we produced a strain deleted for A5 and a revertant strain. Interestingly, the strain deleted for A5 replicated comparably to the wild-type parental strain and induced MCF in rabbits that was indistinguishable from that of the parental strain. The present study is the first to investigate the role of an individual gene of AlHV-1 in MCF pathogenesis.
authors.affiliation: Immunology-Vaccinology (B43b), Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Boudry
authors.affiliation: Immunology-Vaccinology (B43b), Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium.
authors.forename: N
authors.initials: N
authors.lastname: Markine-Goriaynoff
authors.affiliation: Immunology-Vaccinology (B43b), Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Delforge
authors.affiliation: Institute of Interdisciplinary Research in Human and Molecular Biology (IRIBHM), Free University of Brussels, Erasme, 808 Route de Lennik, B-1070 Brussels, Belgium.
authors.forename: J-Y
authors.initials: JY
authors.lastname: Springael
authors.affiliation: Department of Pathology (B23), Faculty of Medicine, University of Liège, B-4000 Liège, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: de Leval
authors.affiliation: Animal facility (B23), University of Liège, B-4000 Liège, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Drion
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Penicuik, Midlothian EH26 0PZ, UK.
authors.forename: G
authors.initials: G
authors.lastname: Russell
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Penicuik, Midlothian EH26 0PZ, UK.
authors.forename: D M
authors.initials: DM
authors.lastname: Haig
authors.affiliation: Immunology-Vaccinology (B43b), Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium.
authors.forename: A F
authors.initials: AF
authors.lastname: Vanderplasschen
authors.affiliation: Immunology-Vaccinology (B43b), Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Dewals
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D044385:GTP-Binding Protein alpha Subunits / Q000378:metabolism; D018142:Gammaherpesvirinae / Q000472:pathogenicity / Q000502:physiology*; D005814:Genes, Viral / Q000502:physiology*; D008304:Malignant Catarrh / Q000378:metabolism* / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011817:Rabbits; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D014774:Virulence; D014779:Virus Replication
chemical_list: D044385:GTP-Binding Protein alpha Subunits; D043562:Receptors, G-Protein-Coupled