title: [Expression of tumor necrosis factor related apoptosis inducing ligand and its receptor in thymus during early post-burn stage in rat with severe burns].
abstract: OBJECTIVE
  To investigate the effect of tumor necrosis factor related apoptosis inducing ligand (TRAIL) and its receptor on apoptosis in thymus during early post-burn stage in rat with severe burns.
  
  
  METHODS
  Fifty Wistar rats were randomly divided into sham scald group (SS, n = 10) and burn group (n = 40). The apoptosis in thymus in rats was detected with annexin V/FITC-PI double staining at 4, 12, 24, 48 post-burn hours (PBH). The expression of TRAIL death receptor DR5, DR4 and its decoy receptor DcR1, DcR2 in thymus were detected by RT-PCR and Western blot at above time-points.
  
  
  RESULTS
  Compared with that in SS group (6.7 +/- 0.8)%, the apoptosis in the thymus in burn group started to increase at 4 PBH [(17.1 +/- 0.4)%], peaked at 12 PBH [(25.2 +/- 1.1)%], and it was still evidently higher than that in SS group at 48 PBH (P < 0.05). There was no obvious difference in the apoptosis rate in rats in burn group among all the time-points. The expression of DR5 in burn group at each time-points was significantly higher than those in SS group, while that of DcR2 shown an opposite tendency (P < 0.05). The expression of DR4, DcR1 was similar in both groups.
  
  
  CONCLUSIONS
  The marked increase in apoptosis rate in rat thymus at early post-burn stage, and the significant change in the expression of DR5 and DcR2 show that TRAIL pathway may participate in apoptosis.
authors.affiliation: Department of Burns and Plastic Surgery, Medical School Hospital of Qingdao University, Qingdao 266003, PR China.
authors.forename: Xiang-Feng
authors.initials: XF
authors.lastname: Leng
authors.forename: De-Chang
authors.initials: DC
authors.lastname: Wang
authors.forename: Qiu
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Feng
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Hu
authors.initials: H
authors.lastname: Xiao
authors.forename: Ran
authors.initials: R
authors.lastname: Huo
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Xue
mesh_terms: D000818:Animals; D017209:Apoptosis; D002056:Burns / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics / Q000378:metabolism*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics / Q000378:metabolism*; D013950:Thymus Gland / Q000378:metabolism*
chemical_list: D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D053221:TNF-Related Apoptosis-Inducing Ligand