title: [Simultaneous determination of peimine, peiminine and zhebeinine in Fritillaria thunbergii from different habitat by HPLC-ELSD].
abstract: OBJECTIVE
  To determine peimine, peiminine and zhebeinine in Fritillaria thunbergii simultaneously by RP-HPLC-ELSD.
  
  
  METHODS
  The HPLC was carried out with a Waters Alliance, Model 2690, equipped with an Xterra RP18 column (3.9 mm x 150 mm, .5 microm), and evaporated light scattering detector. It was eluted gradiently with acetonitrile and 10 mmol x L(-1) NH4HCO3 (adjusted to pH 10. 10 by ammonia solution).
  
  
  RESULTS
  The average recovery rates (n=5) of peimine, peiminine and zhebeinine were 98.2% (RSD 1.7%), 98.2% (RSD 2.2%), 96.5% (RSD 1.3%) respectively.
  
  
  CONCLUSIONS
  The method is simple, sensitive and reliable. It can be used for quantitative determination of F. thunbergii.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xue
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
mesh_terms: D002595:Cevanes / Q000032:analysis*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D017753:Ecosystem; D031410:Fritillaria / Q000737:chemistry* / Q000254:growth & development; D008027:Light; D010946:Plants, Medicinal / Q000737:chemistry / Q000254:growth & development; D015203:Reproducibility of Results; D012542:Scattering, Radiation
chemical_list: D002595:Cevanes; C071593:zhebeinine; C014242:verticine; C047331:peiminine