title: Supramolecular complex formation by beta-cyclodextrin and ferrocenylnaphthalene diimide-intercalated double stranded DNA and improved electrochemical gene detection.
abstract: Ferrocenylnaphthalene diimide 1 can bind to double stranded DNA (dsDNA) by the threading intercalation mode and the resulting complex was stabilized further by beta- cyclodextrin (CD) by forming a supramolecular complex. These complex formation processes were studied by spectroscopic, viscometric, and electrochemical means in the absence or presence of beta-CD. Quantitative analysis by quartz crystal microbalance (QCM) and electrochemical experiments strongly suggested a 2:1 binding stoichiometry for beta-CD to 1 threading-intercalated to the dsDNA-immobilized electrode. Owing to this supramolecular complex formation, electrochemical DNA detection based on 1 was improved considerably.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Sato
authors.forename: Takahiko
authors.initials: T
authors.lastname: Nojima
authors.forename: Michinori
authors.initials: M
authors.lastname: Waki
authors.forename: Shigeori
authors.initials: S
authors.lastname: Takenaka
mesh_terms: D004247:DNA / Q000032:analysis* / Q000378:metabolism; D015342:DNA Probes; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005296:Ferrous Compounds; D007094:Imides; D007364:Intercalating Agents; D046911:Macromolecular Substances; D047392:beta-Cyclodextrins
chemical_list: D015342:DNA Probes; D005296:Ferrous Compounds; D007094:Imides; D007364:Intercalating Agents; D046911:Macromolecular Substances; D047392:beta-Cyclodextrins; C406363:ferrocenylnaphthalene diimide; D004247:DNA; C031215:betadex