Effects of residual fluorescence on time-resolved signals simulated with the finite element method in biological tissues.
A computational model based on finite element method is derived to examine how the simulated time-dependent signals are related to the presence of residual fluorescence in biological media surrounding a fluorescent object. We apply a subtraction technique on recorded data when imperfect uptake of fluorescing agent into the tumor is considered. We show the limits of the subtracting method for low target: background fluorescent absorption contrast by extracting the time to reach the half maximum and analyzing the maximum of the time-resolved signals versus target depth.
Kervella
Marine
M
Groupe ISAIP-ESAIP, 18 rue du 8 mai 1945, BP 80022, 49180 Saint Barthélémy d'Anjou cedex, France. mkervella@esaip.org
Humeau
Anne
A
L'Huillier
Jean-Pierre
JP
D000818:Animals; D003198:Computer Simulation; D003287:Contrast Media / Q000737:chemistry / Q000378:metabolism*; D020342:Finite Element Analysis; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008954:Models, Biological; D041622:Tomography, Optical / Q000379:methods*
D003287:Contrast Media; D005456:Fluorescent Dyes