title: Misidentification of Bordetella bronchiseptica as Bordetella pertussis using a newly described real-time PCR targeting the pertactin gene.
abstract: Recently, a real-time PCR (RT-PCR) assay based on sequence from the gene for pertactin was proposed for identification of Bordetella pertussis. Here, it is reported that the B. pertussis pertactin gene sequence for the region that encompasses the RT-PCR probe and primers is nearly identical to that of many Bordetella bronchiseptica strains of human and avian origin. Additionally, it is demonstrated that such strains are erroneously identified as B. pertussis using the RT-PCR assay. These data suggest that the use of the assay without confirmatory testing may result in erroneous identification of a significant proportion of human isolates of B. bronchiseptica as B. pertussis.
authors.affiliation: Respiratory Diseases of Livestock Research Unit, USDA/Agricultural Research Service/National Animal Disease Center, Ames, IA 50010, USA.
authors.forename: Karen B
authors.initials: KB
authors.lastname: Register
authors.affiliation: Respiratory Diseases of Livestock Research Unit, USDA/Agricultural Research Service/National Animal Disease Center, Ames, IA 50010, USA.
authors.forename: Tracy L
authors.initials: TL
authors.lastname: Nicholson
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001885:Bordetella Infections / Q000175:diagnosis* / Q000382:microbiology; D016950:Bordetella bronchiseptica / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001886:Bordetella pertussis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003937:Diagnosis, Differential; D006801:Humans; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D010566:Virulence Factors, Bordetella / Q000235:genetics*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D010566:Virulence Factors, Bordetella; C068929:pertactin