title: Decolorization of some azo dyes by immobilized Geotrichum sp. biomass in fluidized bed bioreactor.
abstract: Geotrichum sp. strain, which is able to decolorize azo dyes enzymatically, was used in this study for decolorization of synthetics solutions contaminated by toxic azo dyes orange G, trypan blue, azorubine, and methyl red. The biomass of Geotrichum sp. was immobilized in calcium alginate and polyacrylamide gels and used for the decolorization of tested azo dyes in fluidized bed bioreactor. The highest specific decolorization rate was obtained when the fungal biomass was entrapped in calcium alginate beads. Immobilized biomass in calcium alginate continuously decolorized azo dyes after eight repeated batch decolorization experiments without significant loss of activity whereas polyacrylamide immobilized biomass retained only 10% of its activity after 4 days of incubation. The effects of some physicochemical parameters such as temperature, pH, and dyes concentration on decolorization performance of isolated fungal strain were also investigated.
authors.affiliation: Laboratory of Enzyme Technology, College of Natural Science, Chonbuk National University, Chonju, 561-756, Republic of Korea.
authors.forename: Youssef
authors.initials: Y
authors.lastname: Zeroual
authors.forename: Beom Su
authors.initials: BS
authors.lastname: Kim
authors.forename: Myoung Won
authors.initials: MW
authors.lastname: Yang
authors.forename: Mohamed
authors.initials: M
authors.lastname: Blaghen
authors.forename: Kang Min
authors.initials: KM
authors.lastname: Lee
mesh_terms: D000464:Alginates / Q000737:chemistry; D001391:Azo Compounds / Q000737:chemistry* / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D018914:Cells, Immobilized / Q000378:metabolism; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism; D005848:Geotrichum / Q000166:cytology / Q000378:metabolism*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D013997:Time Factors
chemical_list: D000464:Alginates; D001391:Azo Compounds; D004396:Coloring Agents; D006603:Hexuronic Acids; D020723:Glucuronic Acid