title: Anti-chromatin antibodies drive in vivo antigen-specific activation and somatic hypermutation of rheumatoid factor B cells at extrafollicular sites.
abstract: A dominant type of spontaneous autoreactive B cell activation in murine lupus is the extrafollicular generation of plasmablasts. The factors governing such activation have been difficult to identify due to the stochastic onset and chronic nature of the response. Thus, the ability to induce a similar autoreactive B cell response with a known autoantigen in vivo would be a powerful tool in deciphering how autoimmune responses are initiated. We report here the establishment and characterization of a system to initiate autoreactive extrafollicular B cell responses, using IgG anti-chromatin antibodies, that closely mirrors the spontaneous response. We demonstrate that exogenously administered anti-chromatin antibody, presumably by forming immune complexes with released nuclear material, drives activation of rheumatoid factor B cells in AM14 Tg mice. Anti-chromatin elicits autoreactive B cell activation and development into antibody-forming cells at the T zone/red pulp border. Plasmablast generation occurs equally in BALB/c, MRL/+ and MRL/lpr mice, indicating that an autoimmune-prone genetic background is not required for the induced response. Importantly, infused IgG anti-chromatin induces somatic hypermutation in the absence of a GC response, thus proving the extrafollicular somatic hypermutation pathway. This system provides a window on the initiation of an autoantibody response and reveals authentic initiators of it.
authors.affiliation: Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520-8035, USA.
authors.forename: Robin A
authors.initials: RA
authors.lastname: Herlands
authors.forename: Jacqueline
authors.initials: J
authors.lastname: William
authors.forename: Uri
authors.initials: U
authors.lastname: Hershberg
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Shlomchik
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology*; D015551:Autoimmunity / Q000235:genetics / Q000276:immunology*; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D002843:Chromatin / Q000276:immunology; D004195:Disease Models, Animal; D020022:Genetic Predisposition to Disease; D007074:Immunoglobulin G / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000235:genetics / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D008211:Lymphocyte Cooperation; D051379:Mice; D008807:Mice, Inbred BALB C; D019463:Mice, Inbred MRL lpr / Q000276:immunology; D008822:Mice, Transgenic; D009707:Nucleosomes / Q000276:immunology; D009928:Organ Specificity; D010316:Particle Size; D012217:Rheumatoid Factor / Q000276:immunology*; D027041:Somatic Hypermutation, Immunoglobulin / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000276:immunology* / Q000648:ultrastructure; D051193:Toll-Like Receptors / Q000276:immunology
chemical_list: D000974:Antibodies, Antinuclear; D002843:Chromatin; D007074:Immunoglobulin G; D009707:Nucleosomes; D051193:Toll-Like Receptors; D012217:Rheumatoid Factor