Identification and elimination of false-positives in an ELISA-based system for qualitative assessment of glycoconjugate binding using a selection of plant lectins.
Systematic optimization of a lectin-based enzyme-linked immunosorbent assay (ELISA) procedure using a panel of 21 biotinylated plant lectins and a glycoprotein with defined glycosylation (i) identified blocking as a limiting step in solid phase sugar binding analysis and (ii) found Synblock to be a better alternative to bovine serum albumin (BSA) as blocking agent.
Afrough
Babak
B
University of Westminster, London, UK.
Dwek
Miriam V
MV
Greenwell
Pamela
P
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005189:False Positive Reactions; D006001:Glycoconjugates / Q000032:analysis* / Q000378:metabolism*; D037121:Plant Lectins / Q000032:analysis* / Q000378:metabolism*; D011485:Protein Binding; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D006001:Glycoconjugates; D037121:Plant Lectins