title: Differential effects of human chorionic gonadotropin and decidualization on insulin-like growth factors-I and -II in human endometrial stromal cells.
abstract: OBJECTIVE
  To investigate insulin-like growth factors (IGF)-I and -II in human endometrial stromal cells during decidualization in vitro and under the influence of hCG.
  
  
  METHODS
  In vitro experiment.
  
  
  METHODS
  Research laboratory at a medical university center.
  
  
  METHODS
  Premenopausal women undergoing hysterectomy for benign reasons.
  
  
  METHODS
  Endometrial stromal cells from hysterectomy specimens were isolated and incubated with 17beta-E(2), P, and recombinant hCG.
  
  
  METHODS
  Insulin-like growth factor-I and -II messenger RNA and protein were measured by semiquantitative real-time reverse transcriptase polymerase chain reaction and ELISA.
  
  
  RESULTS
  During decidualization in vitro there was a significant increase of IGF-I level between day 0 and 3, followed by a decrease from day 6 to 9. Insulin-like growth factor-II level, however, was significantly reduced during all days of decidualization compared with day 0. In decidualized endometrial stromal cells, hCG decreased IGF-I in a dose-dependent manner without influencing IGF-II.
  
  
  CONCLUSIONS
  Endometrial IGF-I and -II are differentially regulated during decidualization in vitro, suggesting different roles during the differentiation of endometrial stromal cells. Human chorionic gonadotropin as one of the first hormonal signals of the embryo selectively reduces IGF-I and may therefore contribute to the modulation of endometrial receptivity and differentiation during the process of early implantation.
authors.affiliation: Department of Obstetrics and Gynecology, University of Greifswald, Greifswald, Germany.
authors.forename: Herbert
authors.initials: H
authors.lastname: Fluhr
authors.forename: Sabine
authors.initials: S
authors.lastname: Carli
authors.forename: Margarita
authors.initials: M
authors.lastname: Deperschmidt
authors.forename: Diethelm
authors.initials: D
authors.lastname: Wallwiener
authors.forename: Marek
authors.initials: M
authors.lastname: Zygmunt
authors.forename: Peter
authors.initials: P
authors.lastname: Licht
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000235:genetics / Q000378:metabolism*; D003656:Decidua / Q000166:cytology* / Q000502:physiology*; D004717:Endometrium / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics* / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D006063:Chorionic Gonadotropin; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II