Enzyme-linked immunosorbent assay for nonylphenol using antibody-bound microfluid filters in vertical fluidic operation.
We developed an enzyme-linked immunosorbent assay for an endocrine disrupter, nonylphenol, using a microreactor composed of two reaction vessels stacked vertically through a microfluid filter. The filters constructed by deep X-ray lithography possessed 2100 through-bores (phi40 x 200 microm) in polymethylmethacrylate sheets (phi3 mm), which are appropriate for biochemical reactions. Through the optimization of the immunoassay, nonylphenol was quantitatively detected at the range of 0.1-10 ng/ml.
Matsui
Katsuhiro
K
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo 671-2280, Japan.
Kawaji
Isao
I
Utsumi
Yuichi
Y
Ukita
Yoshiaki
Y
Asano
Toshifumi
T
Takeo
Masahiro
M
Kato
Dai-Ichiro
D
Negoro
Seiji
S
D000936:Antigen-Antibody Complex / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D010636:Phenols / Q000032:analysis* / Q000276:immunology; D014462:Ultrafiltration / Q000295:instrumentation* / Q000379:methods
D000936:Antigen-Antibody Complex; D004785:Environmental Pollutants; D010636:Phenols; C025256:nonylphenol