title: Visual snapshots of intracellular kinase activity at the onset of mitosis.
abstract: Visual snapshots of intracellular kinase activity can be acquired with exquisite temporal control by using a light-activatable (caged) sensor, thereby providing a means to interrogate enzymatic activity at any point during the cell-division cycle. Robust protein kinase activity transpires just prior to, but not immediately after, nuclear envelope breakdown (NEB). Furthermore, kinase activity is required for the progression from prophase into metaphase. Finally, the application of selective protein kinase C (PKC) inhibitors, in combination with the caged sensor, correlates the action of the PKC beta isoform with subsequent NEB.
authors.affiliation: Department of Biochemistry, The Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
authors.forename: Zhaohua
authors.initials: Z
authors.lastname: Dai
authors.forename: Natalya G
authors.initials: NG
authors.lastname: Dulyaninova
authors.forename: Sanjai
authors.initials: S
authors.lastname: Kumar
authors.forename: Anne R
authors.initials: AR
authors.lastname: Bresnick
authors.forename: David S
authors.initials: DS
authors.lastname: Lawrence
mesh_terms: D002460:Cell Line; D008938:Mitosis; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D047428:Protein Kinase Inhibitors; D011494:Protein Kinases