Recognition of longer duplex DNA by cooperative strand invasion.
Peptide nucleic acid is a synthetic DNA mimic in which the sugar-phosphate backbone has been replaced by a peptide backbone. A remarkable feature of PNA is its ability to recognize sequences within duplex DNA by strand invasion. We have previously demonstrated that a PNA targeting six bases within duplex DNA cooperatively binds to 12 base-pair site by strand invasion. We here report an successful extension of the target site size to 18 base pairs without the expense of specificity.
Sugiyama
Toru
T
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan. csugi@mail.ecc.u-tokyo.ac.jp
Imamura
Yasutada
Y
Kurihara
Masaaki
M
Kittaka
Atsushi
A
D020137:Base Pair Mismatch; D004247:DNA / Q000737:chemistry*; D024202:Electrophoretic Mobility Shift Assay; D020135:Peptide Nucleic Acids / Q000737:chemistry*
D020135:Peptide Nucleic Acids; C051802:triplex DNA; D004247:DNA