En face imaging of single cell layers by differential phase-contrast optical coherence microscopy.
Optical coherence microscopy (OCM) is capable of imaging the backscattering potential of a sample with high transversal and axial resolution. We report on a combination of OCM with a differential phase-contrast technique that permits imaging of the subwavelength optical path differences that occur between a narrow beam probing a sample and its surrounding. This technique allows small transversal refractive-index variations close to a selected interface to be seen. We report on the method and present first images of a test sample and a single cell layer. The cells act as phase objects; imaging the phase properties improves the contrast compared with that of intensity images.
Sticker
Markus
M
Pircher
Michael
M
Götzinger
Erich
E
Sattmann
Harald
H
Fercher
Adolf F
AF
Hitzenberger
Christoph K
CK