title: [Analysis of beta-lactams-resistance genes in Pseudomonas aeruginosa in burn ward].
abstract: OBJECTIVE
  To investigate the resistance genes and antibiotic resistance patterns against beta-lactams in Pseudomonas aeruginosa prevalent in burn ward.
  
  
  METHODS
  K-B method was performed to test bacterial resistance patterns against 9 species of beta-lactams in Pseudomonas aeruginosa isolated from wounds and dressings of the patient in burn wards. Seven species of resistance genes against beta-lactams were detected with PCR. Tazobactam-inhibited piperacillin resistance test was performed to study whether the above strains produce extended spectrum beta-lactams.
  
  
  RESULTS
  All 12 strains of bacteria with resistance genes detected were resistant to penicillin and cephalosporins (100%), among them 11 were resistant to all antibiotics. Tazobactam-inhibited piperacillin resistance test demonstrated that all strains with resistance genes were ESBLs.
  
  
  CONCLUSIONS
  High incidence of beta-lactams resistance genes is found in Pseudomonas aeruginosa isolated from burn ward, and they have close relationship with the occurrence of multiple drug-resistance.
authors.affiliation: Department of Microbiology, Centre for Disease Control and Prevention, Ningbo 315010, PR China. songqf@nbcdc.org.cn
authors.forename: Qi-Fa
authors.initials: QF
authors.lastname: Song
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.forename: Hui
authors.initials: H
authors.lastname: Lin
authors.forename: Jing-Ye
authors.initials: JY
authors.lastname: Xu
authors.forename: Chun-Guang
authors.initials: CG
authors.lastname: Jin
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Li
mesh_terms: D002053:Burn Units; D002056:Burns / Q000382:microbiology*; D005798:Genes, Bacterial; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D018440:beta-Lactam Resistance / Q000235:genetics*