title: Inhibition of airway allergic disease by co-administration of flagellin with allergen.
abstract: Bacterial flagellin, which activates Toll-like receptor 5 and cytosolic pattern recognition receptor Ipaf, has a strong immunomodulatory activity. In the present study, we examined whether intranasal co-administration of flagellin with allergen could modulate established airway hyperresponsiveness and Th2 response using an ovalbumin (OVA)-sensitized mouse model. Balb/c mice sensitized with OVA were treated with OVA-flagellin (FlaB) mixture three times at 1-week intervals. Seven days after the final OVA-FlaB administration, the mice were challenged with OVA inhalation, and airway responses and OVA-specific immune responses were evaluated. The OVA-FlaB treatment significantly suppressed OVA-induced airway hyperresponsiveness, airway eosinophilic inflammation, and OVA-specific Th2 cytokine productions in splenocytes. These results indicate that flagellin co-administered with allergen can modulate airway inflammatory response through inhibition of Th2 responses, and flagellin can be considered as a component for allergen-specific immunotherapy.
authors.affiliation: Clinical Vaccine R&D Center, Chonnam National University, Gwangju, South Korea. selee@chonnam.ac.kr
authors.forename: Shee Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Youngil I
authors.initials: YI
authors.lastname: Koh
authors.forename: Mi-Kwang
authors.initials: MK
authors.lastname: Kim
authors.forename: Young Ran
authors.initials: YR
authors.lastname: Kim
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Jong Hee
authors.initials: JH
authors.lastname: Nam
authors.forename: Yoo Duk
authors.initials: YD
authors.lastname: Choi
authors.forename: Soo Jang
authors.initials: SJ
authors.lastname: Bae
authors.forename: Young Jong
authors.initials: YJ
authors.lastname: Ko
authors.forename: Hwa-Ja
authors.initials: HJ
authors.lastname: Ryu
authors.forename: Jeong Tae
authors.initials: JT
authors.lastname: Koh
authors.forename: Hyon E
authors.initials: HE
authors.lastname: Choy
authors.forename: Joon Haeng
authors.initials: JH
authors.lastname: Rhee
mesh_terms: D000485:Allergens / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001985:Bronchial Provocation Tests; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D016207:Cytokines / Q000378:metabolism; D004804:Eosinophils / Q000166:cytology; D005260:Female; D005408:Flagellin / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology*; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007249:Inflammation / Q000175:diagnosis / Q000628:therapy; D008214:Lymphocytes / Q000166:cytology; D016676:Macrophages, Alveolar / Q000166:cytology; D016210:Methacholine Chloride / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000166:cytology; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology; D012123:Pulmonary Ventilation / Q000187:drug effects; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D012130:Respiratory Hypersensitivity / Q000139:chemically induced / Q000503:physiopathology / Q000628:therapy*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D051198:Toll-Like Receptor 5 / Q000819:agonists
chemical_list: D000485:Allergens; D016207:Cytokines; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D051198:Toll-Like Receptor 5; D016210:Methacholine Chloride; D005408:Flagellin; C416532:flaB flagellin; D010047:Ovalbumin