title: Effect of a laser irradiation on the vascularisation of safety and X-ray radiated bone.
abstract: Thermal preconditioning induces a cytoprotective effect and promotes tissue recovering. Laser is an appropriated method to generate a controlled and reproducible heating. Bone healing, a crucial challenge in medicine, is affected by X-ray radiation which induces a chronic antiangiogenic effect. So, this study aims to investigate the role of laser preconditioning on the vascularisation of bone after X-ray radiation. An optical bone chamber allowed the study of the vascularization process. The vascular density (VD) was determined using image processing. A longitudinal study was performed on 20 rabbits divided in four groups: #1: control group (n=5); #2: laser irradiation alone (diode laser 810nm, fluence= 48J/cm2) (n=5). #3: X-ray radiation (18.75Gy) alone (n=5), #4: laser preconditioning 24 hours before a X-ray radiation (n=5). VD remained stable during 12-week follow up for groups #1 and #2. X-ray radiation lead to an important decrease of the superficial bone vascularization in group #3. The decrease of the vascularization was limited in group #4 highlighting a different evolution between group #3 and #4. Those results were confirmed by histological analysis. Our preliminary findings show that laser preconditioning preserves vascularization in X-ray radiated bone site, outlining a novel approach for the bone healing in which the vascular supply has been injured.
authors.affiliation: INSERM French National Institute of Health and Medical Research, Pavillon Vancostenobel, Lille University Hospital CH&U, 59037 Lille Cedex, France. desmons@lille.inserm.fr
authors.forename: Sophie
authors.initials: S
authors.lastname: Desmons
authors.forename: Caroline
authors.initials: C
authors.lastname: Delfosse
authors.forename: Philippe
authors.initials: P
authors.lastname: Rochon
authors.forename: Bruno
authors.initials: B
authors.lastname: Buys
authors.forename: Guillaume
authors.initials: G
authors.lastname: Penel
authors.forename: Serge
authors.initials: S
authors.lastname: Mordon
mesh_terms: D000818:Animals; D004307:Dose-Response Relationship, Radiation; D007834:Lasers; D018919:Neovascularization, Physiologic / Q000502:physiology* / Q000528:radiation effects*; D011817:Rabbits; D011829:Radiation Dosage; D012886:Skull / Q000098:blood supply* / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D014965:X-Rays