title: Short communication: extraction of beta-casein from goat milk.
abstract: Peptides derived from milk beta-casein have potential biological activities, such as antihypertensive and immunostimulating properties. These biological properties increase the demand for the production of specific bioactive peptides. beta-Casein can be isolated directly from renneted skim milk, based on the preferential solubilization of beta-casein at low temperature. This study was conducted to compare the recovery and purity of beta-casein extracted from goat and cow milks. Rennet casein was prepared from both milks, heat treated, and dispersed in demineralized water at various temperatures. beta-Casein recovery in the soluble phase increased with decreasing incubation temperature. Concentration of beta-casein was 43% higher in goat milk than in cow milk, which had a direct effect on beta-casein recovery. Furthermore, beta-casein was extracted more efficiently from goat rennet casein. As a result, the extraction yield of beta-casein was 53% higher in goat milk than in cow milk. The purity of beta-casein extracted from both milks reached approximately 90% after incubation at 0 degrees C.
authors.affiliation: Food Research and Development Centre, Agriculture, and Agri-Food Canada, Saint-Hyacinthe, Quebec, Canada J2S 8E3.
authors.forename: S
authors.initials: S
authors.lastname: Lamothe
authors.forename: G
authors.initials: G
authors.lastname: Robitaille
authors.forename: D
authors.initials: D
authors.lastname: St-Gelais
authors.forename: M
authors.initials: M
authors.lastname: Britten
mesh_terms: D000818:Animals; D002364:Caseins / Q000032:analysis / Q000302:isolation & purification*; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D005260:Female; D006041:Goats; D008892:Milk / Q000737:chemistry*; D012995:Solubility; D013045:Species Specificity; D013696:Temperature
chemical_list: D002364:Caseins