title: Targeted gene disruption for the analysis of virulence of Staphylococcus aureus.
abstract: In recent years, molecular genetic approaches to the study of the disease pathogenesis of Staphylococcus aureus have resulted in many new biological insights. I describe methods used for targeted disruption of staphylococcal genes leading to loss of gene function, important for studies of staphylococcal proteins and their role in virulence.
authors.affiliation: Centre for Infectious Diseases, Chancellor's Building, New Royal Infirmary, University of Edinburgh, Edinburgh, Scotland, UK.
authors.forename: J Ross
authors.initials: JR
authors.lastname: Fitzgerald
mesh_terms: D000483:Alleles; D003001:Cloning, Molecular; D024881:Drug Resistance, Bacterial / Q000235:genetics; D017353:Gene Deletion; D018390:Gene Targeting; D005798:Genes, Bacterial; D016296:Mutagenesis; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D013204:Staphylococcus Phages / Q000235:genetics; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity*; D013696:Temperature; D014170:Transformation, Genetic; D014774:Virulence / Q000235:genetics