title: Translation factor IF2 at the interface of transposition and replication by the PriA-PriC pathway.
abstract: Bacteriophage Mu DNA synthesis is initiated during transposition by replication restart proteins PriA, DnaT and either PriB or PriC. The PriA-PriC pathway requires PriA's helicase activity and other host factors that promote the orderly transition from transpososome to replisome on the Mu DNA template. The host factor MRFalpha-PR, which removes obstacles to PriA binding and promotes the PriA-PriC pathway, was identified to be the translation initiation factor IF2. Purified isoform IF2-2, which is truncated at the N-terminal end, had full MRFalpha-PR activity whereas full-length IF2-1 was inactive. IF2-2 was bound to the Mu DNA template specifically at the step for prereplisome assembly. Prior steps in the orderly transition from transpososome were essential to promote efficient IF2-2 binding. Moreover, PriA helicase activity was subsequently needed to displace IF2-2, remodelling the template to permit replisome assembly. IF2's role in the transition mechanism as well as its function as G protein and translation factor suggest its potential to regulate DNA synthesis by this pathway.
authors.affiliation: Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Rm. 331 Basic Science Bldg., 3900 Reservoir Road NW, Washington, DC 20057-1455, USA.
authors.forename: Stella H
authors.initials: SH
authors.lastname: North
authors.forename: Sandy E
authors.initials: SE
authors.lastname: Kirtland
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakai
mesh_terms: D010583:Bacteriophage mu / Q000235:genetics / Q000378:metabolism; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication; D004591:Electrophoresis, Polyacrylamide Gel; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D039881:Prokaryotic Initiation Factor-2 / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D053719:Tandem Mass Spectrometry
chemical_list: D029968:Escherichia coli Proteins; D039881:Prokaryotic Initiation Factor-2; C069995:PriC protein, E coli; C506743:priA protein, E coli; D004265:DNA Helicases