title: The 20 years of PROSITE.
abstract: PROSITE consists of documentation entries describing protein domains, families and functional sites, as well as associated patterns and profiles to identify them. It is complemented by ProRule, a collection of rules based on profiles and patterns, which increases the discriminatory power of profiles and patterns by providing additional information about functionally and/or structurally critical amino acids. In this article, we describe the implementation of a new method to assign a status to pattern matches, the new PROSITE web page and a new approach to improve the specificity and sensitivity of PROSITE methods. The latest version of PROSITE (release 20.19 of 11 September 2007) contains 1319 patterns, 745 profiles and 764 ProRules. Over the past 2 years, about 200 domains have been added, and now 53% of UniProtKB/Swiss-Prot entries (release 54.2 of 11 September 2007) have a PROSITE match. PROSITE is available on the web at: http://www.expasy.org/prosite/.
authors.affiliation: Swiss Institute of Bioinformatics (SIB), Centre Medical Universitaire, Structural Biology and Bioinformatics Department, University of Geneva, 1 rue Michel Servet, CH-1211 Geneva, Switzerland.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Hulo
authors.forename: Amos
authors.initials: A
authors.lastname: Bairoch
authors.forename: Virginie
authors.initials: V
authors.lastname: Bulliard
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Cerutti
authors.forename: Béatrice A
authors.initials: BA
authors.lastname: Cuche
authors.forename: Edouard
authors.initials: E
authors.lastname: de Castro
authors.forename: Corinne
authors.initials: C
authors.lastname: Lachaize
authors.forename: Petra S
authors.initials: PS
authors.lastname: Langendijk-Genevaux
authors.forename: Christian J A
authors.initials: CJ
authors.lastname: Sigrist
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry / Q000145:classification; D030562:Databases, Protein / Q000266:history; D049673:History, 20th Century; D049674:History, 21st Century; D020407:Internet; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000145:classification*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D012984:Software; D014584:User-Computer Interface
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D011506:Proteins