[Protective effect of amlodipine on the cytotoxicity induced by contrast media in human kidney cells].
OBJECTIVE
To explore the protective effect of amlodipine on the cytotoxicity induced by contrast media (meglumine diatrizoate) in human kidney cells (HKC).


METHODS
An HKC line was used. The experiment was divided into 4 groups: a model group (diatrizoate 111g/L), a prevention group (diatrizoate 111g/L+amlodipine 10(-5)mol/L), an amlodipine control group (amlodipine 10(-5)mol/L), and a culture medium control group (simple none blood serum DMEM-F12 medium). Cytotoxicity induced by meglumine diatrizoate was analysed by methyl thiazolyl tetrazolium (MTT) test, lactate dehydrogenase (LDH) assay, Hochest33258 fluorescence stained cytospins, and flow cytometric DNA analysis. The protein expression of Bax was determined by Western blot, and caspase-3 activity was examined by fluorometric method.


RESULTS
In the prevention group, the cell viability increased significantly (P<0.05), LDH levels decreased (P<0.05), and the apoptosis was lower than that of the model group (P<0.05) .Bax protein expression and caspase 3 activity decreased (P<0.05).


CONCLUSIONS
Amlodipine can inhibit the HKC apoptosis and protect the renal tubule cell from injury induced by meglumine diatrizoate.
Zhou
Xiao-rong
XR
Department of Geriatrics, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Duan
Shao-bin
SB
Peng
You-ming
YM
Liu
Fu-you
FY
Ye
Yun
Y
Liu
Rui-hong
RH
Li
Gui-yuan
GY
D017311:Amlodipine / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D003287:Contrast Media / Q000633:toxicity*; D003974:Diatrizoate Meglumine / Q000633:toxicity; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology* / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology
D003287:Contrast Media; D020011:Protective Agents; D017311:Amlodipine; D003974:Diatrizoate Meglumine; C505407:CASP3 protein, human; D053148:Caspase 3