title: Steroidal glycosides from the rhizomes of Ruscus hypophyllum.
abstract: Seven steroidal glycosides, along with one known glycoside, were isolated from the rhizomes of Ruscus hypophyllum (Liliaceae). Comprehensive spectroscopic analysis, including 2D NMR spectroscopy, and the results of acid hydrolysis allowed the chemical structures of the compounds to be assigned as (23S,25R)-23-hydroxyspirost-5-en-3beta-yl O-alpha-l-rhamnopyranosyl-(1-->4)-beta-d-glucopyranoside (1), 1beta-hydroxyspirosta-5,25(27)-dien-3beta-yl O-alpha-l-rhamnopyranosyl-(1-->4)-beta-d-glucopyranoside (2), (22S)-16beta,22-dihydroxycholest-5-en-3beta-yl O-alpha-l-rhamnopyranosyl-(1-->4)-beta-d-glucopyranoside (3), (22S)-16beta-[(beta-d-glucopyranosyl)oxy]-22-hydroxycholest-5-en-3beta-yl O-alpha-l-rhamnopyranosyl-(1-->4)-beta-d-glucopyranoside (4), (22S)-16beta-[(beta-d-glucopyranosyl)oxy]-22-hydroxycholest-5-en-3beta-yl beta-d-glucopyranoside (5), (22S)-16beta-[(beta-d-glucopyranosyl)oxy]-3beta,22-dihydroxycholest-5-en-1beta-yl O-alpha-l-rhamnopyranosyl-(1-->2)-(3,4-di-O-acetyl-beta-d-xylopyranoside) (6), and (22S)-16beta-[(beta-d-glucopyranosyl)oxy]-3beta,22-dihydroxycholest-5-en-1beta-yl O-alpha-l-rhamnopyranosyl-(1-->2)-O-[beta-d-xylopyranosyl-(1-->3)]-beta-d-xylopyranoside (7), respectively. This is the first isolation of a series of cholestane glycosides from a Ruscus species.
authors.affiliation: Tokyo University of Pharmacy and Life Sciences, School of Pharmacy, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. mimakiy@ps.toyaku.ac.jp
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Mimaki
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Aoki
authors.forename: Maki
authors.initials: M
authors.lastname: Jitsuno
authors.forename: Ceyda Sibel
authors.initials: CS
authors.lastname: Kiliç
authors.forename: Maksut
authors.initials: M
authors.lastname: Coşkun
mesh_terms: D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification; D019668:Liliaceae / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods / Q000592:standards; D015394:Molecular Structure; D012015:Reference Standards; D027343:Rhizome / Q000737:chemistry*; D013045:Species Specificity; D013256:Steroids / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D006027:Glycosides; D013256:Steroids