Rapid changes in amino acid and polyamine metabolism during copper-induced cell death of human gingival fibroblast.
There are very few studies on the interaction between dental alloys and oral tissues. The effect of direct contact with copper (Cu) on the cellular function of human gingival fibroblast (HGF) derived from the periodontal tissues was investigated. When HGF cells were inoculated onto a Cu plate, the viability of HGF cells immediately declined. This was accompanied by vacuolization and chromatin condensation near the nuclear membrane. The intracellular concentration of spermidine and spermine declined, whereas that of putrescine slightly increased. Amino acid analysis of the medium revealed that glutamine was consumed at the greatest rate, amounting to more than half of the total amino acid consumption. Contact with the Cu plate resulted in the complete elimination of glutamine utilization and a simultaneous increase in the production of most amino acids, possibly due to enhanced proteolysis. This was accompanied by a time-dependent increase in the consumption of cystine, possibly due to oxidative reactions, and the enhanced production of glycine and glutamic acid. These data suggest that the contact with the Cu plate induced non-apoptotic cell death in HGF cells, which was tightly coupled with a rapid dysfunction of amino acid and polyamine metabolism.
Sakagami
Hiroshi
H
Division of Pharmacology, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Saitama 350-0283, Japan. sakagami@dent.meikai.ac.jp
Yamazaki
Takashi
T
Onuki
Hiroyuki
H
Yamazaki
Atsushi
A
Hibino
Yasushi
Y
Hashimoto
Ken
K
Kanda
Yumiko
Y
Kunii
Shiro
S
Yokote
Yoshiko
Y
Nakajima
Hiroshi
H
Shimada
Jun
J
D000596:Amino Acids / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D003300:Copper / Q000494:pharmacology*; D005881:Gingiva / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D011073:Polyamines / Q000378:metabolism*; D013997:Time Factors
D000596:Amino Acids; D011073:Polyamines; D003300:Copper