title: Clonal characterization of sporadic cribriform-morular variant of papillary thyroid carcinoma by laser microdissection-based APC mutation analysis.
abstract: Cribriform-morular variant (C-MV) of papillary thyroid carcinoma (PTC) is a rare and unusual neoplasm composed of multiple histologic components, including cribriform, papillary, solid, tall columnar, and morular patterns. Analyses of gross C-MV of PTC lesions has linked adenomatous polyposis coli (APC) mutations to its pathogenesis; however, the extent of involvement of mutations in the development of individual components is unclear. We report on bidirectional sequencing of the mutation cluster region (codons 1032-1565) of the APC gene in individually laser-microdissected components of a previously unreported C-MV of PTC. A silent Thr1493Thr gene variant was found in all tumoral components, whereas a 5-base-pair frameshift deletion at codon 1309 was identified only in the morules. Neither variant was observed in matched normal thyroid tissue. These results show the histologic components of C-MV of PTC to have some common mutational background, although additional somatic mutations may be involved in the development of morular structures.
authors.affiliation: Oncology Research Institute, Centre for Life Sciences, National University of Singapore, Singapore.
authors.forename: Manish Mani
authors.initials: MM
authors.lastname: Subramaniam
authors.forename: Thomas Choudary
authors.initials: TC
authors.lastname: Putti
authors.forename: Diyanah
authors.initials: D
authors.lastname: Anuar
authors.forename: Pei Yi
authors.initials: PY
authors.lastname: Chong
authors.forename: Nilesh
authors.initials: N
authors.lastname: Shah
authors.forename: Manuel
authors.initials: M
authors.lastname: Salto-Tellez
authors.forename: Richie
authors.initials: R
authors.lastname: Soong
mesh_terms: D000231:Adenocarcinoma, Papillary / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D000328:Adult; D001483:Base Sequence; D002999:Clone Cells; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D017491:Genes, APC; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D008969:Molecular Sequence Data; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery
chemical_list: D004273:DNA, Neoplasm