Hidden VIM-1 metallo-beta-lactamase phenotypes among Acinetobacter baumannii clinical isolates.
A total of 87 Acinetobacter baumannii nonrepetitive consecutive clinical isolates were tested for the presence of metallo-beta-lactamases (MBLs). Results of phenotypic assays (MBL Etest, imipenem/imipenem-EDTA combined-disk test, and imipenem/EDTA double-disk synergy test) were negative in all cases, but molecular testing revealed the presence of two bla(VIM-1)-carrying isolates. One isolate had bla(VIM-1) preceded by a weak P1 promoter, and both had inactivated P2 promoters and reduced bla(VIM-1) expression, partially justifying the results revealing hidden MBL phenotypes.
Ikonomidis
Alexandros
A
Department of Microbiology, Medical School, University of Thessaly, Larissa, Greece.
Ntokou
Eleni
E
Maniatis
Antonios N
AN
Tsakris
Athanassios
A
Pournaras
Spyros
S
D000151:Acinetobacter Infections / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000379:methods; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics; D047090:beta-Lactams / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D047090:beta-Lactams; C417264:VIM-1 metallo-beta-lactamase; D001618:beta-Lactamases