title: [Study on the biological function of vascular endothelial cells in the hypertrophic scar].
abstract: OBJECTIVE
  To explore the biological function of vascular endothelial cells from hypertrophic scar, and to analyze the relationship between them.
  
  
  METHODS
  The samples from human hypertrophic scar and normal skin tissue were harvested for histological examination. Then vascular endothelial cells were purified and isolated from the samples, and the level of transforming growth factor (TGF) beta1, platelet derived growth factor (PDGF), endothelin1 (ET)-1, fibroblast growth factor (FGF)2 and vascular endothelial growth factor (VEGF) were determined in a single cell with ELISA.
  
  
  RESULTS
  Few capillary vessels were observed in normal skin under microscope, while an increased number of them were present in hypertrophic scar, with slender, tortuous in morphology and even occluded. The diameter of blood capillary in hypertrophic scar was tiny under electron microscope, and the exfoliation of endothelial cells was observed. The levels of TGF-beta1, PDGF, ET-1, bFGF and VEGF from vascular endothelial cells from hypertrophic scar were 60 +/- 8, 30 +/- 4, 0.12 +/- 0.03, 52 +/- 5, 18.1 +/- 1.2 microg/cell, respectively, which were obviously lower than those in normal skin (P < 0.05).
  
  
  CONCLUSIONS
  The biological function of vascular endothelial cells was attenuated in the hypertrophic scar, which mightbe the result of the production of large amounts of collagen in the scar tissue, as well as hypoxia.
authors.affiliation: Department of Burns, Ruijin Hospital, Shanghai Jiao Tong University, Shanghai 200025, PR China.
authors.forename: Xi-Qiao
authors.initials: XQ
authors.lastname: Wang
authors.forename: Shu-Liang
authors.initials: SL
authors.lastname: Lu
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Mao
authors.forename: Ying-Kai
authors.initials: YK
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000378:metabolism* / Q000473:pathology*; D003094:Collagen / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism*; D019332:Endothelin-1 / Q000378:metabolism; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006801:Humans; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D012867:Skin / Q000098:blood supply*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing; D055815:Young Adult
chemical_list: D019332:Endothelin-1; D010982:Platelet-Derived Growth Factor; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D003094:Collagen