A simple and cost effective method for the quantification of 8-hydroxy-2'-deoxyguanosine from urine using liquid chromatography tandem mass spectrometry.
A rapid and high-throughput method for the determination of urinary levels of the oxidative stress biomarker, 8-hydroxy-2'-deoxyguanosine (8-OH-dG), has been developed and validated using liquid chromatography combined with electrospray ionization tandem mass spectrometry (LC-MS/MS). The assay features a cheap and readily available non-isotopic internal standard, a single-step filtration sample preparation, and a total analysis time of 6 min including column re-equilibration. The method was validated based on linearity, accuracy (100-106%), precision (CV < 7%), sample preparation stability (< or =5%, 72 h). Intra-laboratory patient ranges were established comparing children and adults (n = 345).
Crow
Brian
B
Metametrix Clinical Laboratory, 3425 Corporate Way, Duluth, GA 30096, USA. bcrow@metametrix.com
Bishop
Michael
M
Kovalcik
Kasey
K
Norton
Dean
D
George
Joe
J
Bralley
J Alexander
JA
D000080242:8-Hydroxy-2'-Deoxyguanosine; D002853:Chromatography, Liquid / Q000379:methods*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000652:urine; D006801:Humans; D015394:Molecular Structure; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine