Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli.
ZnuA is the periplasmic Zn(2+)-binding protein associated with the high-affinity ATP-binding cassette ZnuABC transporter from Escherichia coli. Although several structures of ZnuA and its homologs have been determined, details regarding metal ion stoichiometry, affinity, and specificity as well as the mechanism of metal uptake and transfer remain unclear. The crystal structures of E. coli ZnuA (Eco-ZnuA) in the apo, Zn(2+)-bound, and Co(2+)-bound forms have been determined. ZnZnuA binds at least two metal ions. The first, observed previously in other structures, is coordinated tetrahedrally by Glu59, His60, His143, and His207. Replacement of Zn(2+) with Co(2+) results in almost identical coordination geometry at this site. The second metal binding site involves His224 and several yet to be identified residues from the His-rich loop that is unique to Zn(2+) periplasmic metal binding receptors. Electron paramagnetic resonance and X-ray absorption spectroscopic data on CoZnuA provide additional insight into possible residues involved in this second site. The second site is also detected by metal analysis and circular dichroism (CD) titrations. Eco-ZnuA binds Zn(2+) (estimated K (d) < 20 nM), Co(2+), Ni(2+), Cu(2+), Cu(+), and Cd(2+), but not Mn(2+). Finally, conformational changes upon metal binding observed in the crystal structures together with fluorescence and CD data indicate that only Zn(2+) substantially stabilizes ZnuA and might facilitate recognition of ZnuB and subsequent metal transfer.
Yatsunyk
Liliya A
LA
Department of Biochemistry, Northwestern University, Evanston, IL, 60208, USA. amyr@northwestern.edu.
Easton
J Allen
JA
Kim
Lydia R
LR
Sugarbaker
Stacy A
SA
Bennett
Brian
B
Breece
Robert M
RM
Vorontsov
Ivan I
II
Tierney
David L
DL
Crowder
Michael W
MW
Rosenzweig
Amy C
AC
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry* / Q000378:metabolism*; D000042:Absorption; D000817:Anilino Naphthalenesulfonates / Q000737:chemistry; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D002942:Circular Dichroism; D003035:Cobalt / Q000378:metabolism; D018360:Crystallography, X-Ray; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism*; D005453:Fluorescence; D008670:Metals / Q000378:metabolism*; D008958:Models, Molecular; D019897:Periplasm / Q000378:metabolism*; D017433:Protein Structure, Secondary; D013056:Spectrophotometry, Ultraviolet; D013379:Substrate Specificity; D015032:Zinc / Q000378:metabolism
C515594:8-anilino-1-naphthalenesulfonic acid; D018528:ATP-Binding Cassette Transporters; D000817:Anilino Naphthalenesulfonates; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D008670:Metals; C488663:ZnuA protein, E coli; C017800:zinc-binding protein; D003035:Cobalt; D015032:Zinc