Upregulation of discoidin domain receptor 2 in nasopharyngeal carcinoma.
BACKGROUND
Nasopharyngeal carcinoma (NPC) is associated with Epstein-Barr virus (EBV) and has high metastatic potential. Discoidin domain receptors (DDR1, DDR2) are receptor-type tyrosine kinases activated by collagen. Their ability to induce expression of matrix metalloproteinase is related with tumor invasion. Therefore, we aim to investigate DDRs gene expression and its regulation in NPC.


RESULTS
By use of real-time quantitative polymerase chain reaction (Q-PCR), DDR2 gene expression but not DDR1 was significantly higher in primary and metastatic NPC. DDR2 was predominantly distributed in NPC tumor cells rather than in infiltrating lymphocytes. EBV Z-transactivator (Zta) transfection may distinctly elevate DDR2 level. Furthermore, data from reporter assay indicate that Zta could transactivate DDR2 promoter activity, suggesting the possible upregulation mechanism.


CONCLUSIONS
DDR2 was differentially upregulated in NPC and modulated by EBV Zta protein. DDR2 may play a role in NPC invasion and serve as a diagnostic and therapeutic target.
Chua
Huey-Huey
HH
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Number 1, Jen-Ai Road, Section 1, Taipei 10051, Taiwan, Republic of China. chtsai@ha.mc.ntu.edu.tw
Yeh
Te-Huei
TH
Wang
Ying-Piao
YP
Huang
Yu-Tzu
YT
Sheen
Tzung-Shiahn
TS
Lo
You-Chang
YC
Chou
Ya-Ching
YC
Tsai
Ching-Hwa
CH
D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism; D000070818:Discoidin Domain Receptors; D004847:Epithelial Cells / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000378:metabolism; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D011975:Receptors, Mitogen / Q000235:genetics* / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D015854:Up-Regulation / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
C063676:BZLF1 protein, Herpesvirus 4, Human; D011975:Receptors, Mitogen; D015534:Trans-Activators; D014764:Viral Proteins; D000070818:Discoidin Domain Receptors; D020794:Receptor Protein-Tyrosine Kinases