Repair mechanism of DNA-protein cross-link damage in Escherichia coli.
DNA-protein cross-link (DPC) damage is produced by varieties of genotoxic agents such as ionizing radiation, UV light, aldehydes, and some chemotherapeutic compounds. DPCs would be harmful to cells since proteins immobilized on DNA strands could hamper DNA transactions in cells. However, it has not been clarified how DPCs are repaired in cells. In the present study, we prepared DNA substrates containing defined DPCs and tested them for repair enzymes. We also examined the sensitivity of Escherichia coli to DPC-inducing agents.
Nakano
Toshiaki
T
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
Morishita
Soh
S
Terato
Hiroaki
H
Pack
Seung Pil
SP
Makino
Keisuke
K
Ide
Hiroshi
H
D003432:Cross-Linking Reagents / Q000633:toxicity; D018736:DNA Adducts / Q000737:chemistry* / Q000378:metabolism; D004249:DNA Damage; D045647:DNA Glycosylases / Q000378:metabolism; D004260:DNA Repair; D045643:DNA Repair Enzymes / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005557:Formaldehyde / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D011684:Purine Nucleosides / Q000737:chemistry*
D003432:Cross-Linking Reagents; D018736:DNA Adducts; D011684:Purine Nucleosides; C468766:oxanine; D005557:Formaldehyde; D045647:DNA Glycosylases; C473634:NEIL1 protein, human; D045643:DNA Repair Enzymes