title: Sensitive and rapid detection of Aeromonas caviae in stool samples by loop-mediated isothermal amplification.
abstract: In this study, a loop-mediated isothermal amplification assay targeting the 16S-23S intergenic spacer regions (internal transcribed spacer) of Aeromonas caviae was developed. Eighteen reference strains and 109 clinical samples were analyzed. The results showed this detection technique is more reliable and convenient compared with common polymerase chain reaction and biochemical culture methods.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Science, Nankai University, Tianjin 300071, China.
authors.forename: Xiao Na
authors.initials: XN
authors.lastname: Wei
authors.forename: Ze Jun
authors.initials: ZJ
authors.lastname: Zheng
authors.forename: Li Huan
authors.initials: LH
authors.lastname: Zhang
authors.forename: Fen
authors.initials: F
authors.lastname: Qu
authors.forename: Xitai
authors.initials: X
authors.lastname: Huang
mesh_terms: D000333:Aeromonas / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D021901:DNA, Intergenic