[Effect of calcitonin-gene-related peptide on MMP-9 production in human bronchial epithelial cells].
OBJECTIVE
To examine the expression of matrix metalloproteinase-9 (MMP-9) in human bronchial epithelial cells treated with calcitonin-gene-related peptide (CGRP).


METHODS
RT-PCR and gelatin zymography were performed to examine the dynamic expression and activity of MMP-9 in human bronchial epithelial cells at different doses (10(-10), 10(-9), 10(-8), 10(-7), and 10(-6)mol/L) and different time points (6,12,18,24,36, and 48h) after the stimulation of CGRP.


RESULTS
The unstimulated human bronchial epithelial cells only secreted a small amount of MMP-9. After the CGRP stimulation, the expression of MMP-9 presented in a concentration-dependent (10(-10), 10(-9), 10(-8), 10(-7), and 10(-6) mol/L) and time-dependent (6,12,18,24,36, and 48 h) manners (P<0.01) in human bronchial epithelial cells. The effect of CGRP could be diminished by H-7 and W-7, an antagonist of protein kinase C (PKC) and calmodulin (CaM) (P<0.05).


CONCLUSIONS
CGRP can stimulate the secretion and expression of MMP-9 in human bronchial epithelial cells, and the signal transduction is partly via the PKC and CaM pathway.
Guan
Cha-xiang
CX
Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410078,China. guanchaxiang@xysm.net
Liu
Yong-ping
YP
Cui
Yan-ru
YR
Yu
Fang
F
Sun
Guo-ying
GY
Liu
Hui-jun
HJ
D001980:Bronchi / Q000166:cytology; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002147:Calmodulin / Q000378:metabolism; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D015398:Signal Transduction
D002147:Calmodulin; D011493:Protein Kinase C; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9; D015740:Calcitonin Gene-Related Peptide