Specific detection of PCR product from Legionella pneumophila strain Philadelphia1 using zinc finger protein Sp2.
We have developed a novel method to detect PCR products from pathogen genome DNA by Zinc finger protein that can bind to double strand DNA (dsDNA) in sequence specific manner. In this study, we tried to detect Legionella pneumophila strain Philadelphia 1 using Zinc finger protein. We found the specific target DNA sequence for zinc finger protein Sp2 in L. pneumophila strain Philadelphia 1 genome DNA. Specific PCR product was successfully amplified from L. pneumophila strain Philadelphia 1 genome DNA and we used Zinc finger protein Sp2 to detect it. We succeeded in detecting the PCR products from L. pneumophila strain Philadelphia 1 genome DNA with Sp2.
Ikebukuro
Kazunori
K
Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture & Technology, 2-24-16 Naka-machi, Koganei, Tokyo 184-8588, Japan.
Kumagai
Takenori
T
Motoki
Hiroaki
H
Osawa
Yuko
Y
Matuo
Takafumi
T
Horiuchi
Michio
M
Sode
Koji
K
D001483:Base Sequence; D001665:Binding Sites; D016952:Legionella pneumophila / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051704:Sp2 Transcription Factor / Q000737:chemistry / Q000378:metabolism*; D016335:Zinc Fingers
D051704:Sp2 Transcription Factor