Targeted gene disruption for the analysis of virulence of Staphylococcus aureus.
In recent years, molecular genetic approaches to the study of the disease pathogenesis of Staphylococcus aureus have resulted in many new biological insights. I describe methods used for targeted disruption of staphylococcal genes leading to loss of gene function, important for studies of staphylococcal proteins and their role in virulence.
Fitzgerald
J Ross
JR
Centre for Infectious Diseases, Chancellor's Building, New Royal Infirmary, University of Edinburgh, Edinburgh, Scotland, UK.
D000483:Alleles; D003001:Cloning, Molecular; D024881:Drug Resistance, Bacterial / Q000235:genetics; D017353:Gene Deletion; D018390:Gene Targeting; D005798:Genes, Bacterial; D016296:Mutagenesis; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D013204:Staphylococcus Phages / Q000235:genetics; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity*; D013696:Temperature; D014170:Transformation, Genetic; D014774:Virulence / Q000235:genetics