Significance of urinary excretion of 8-hydroxy-2'-deoxyguanosine in healthy subjects and liver disease patients.
OBJECTIVE
Although the importance of reactive oxygen species (ROS) in the pathogenesis of various diseases is stressed, clinical significance of the markers reflecting DNA oxidation such as 8-hydroxy-2'-deoxyguanosine (8-OHdG) remains to be clarified.


METHODS
To examine clinical usefulness of 8-OHdG in healthy individuals in comparison with liver disease patients, urinary excretion of 8-OHdG was measured in 336 healthy individuals and 110 patients with liver disease.


RESULTS
In healthy persons, the 8-OHdG excretion was increased in an age-dependent manner. It was positively correlated with cigarettes smoked a day and negatively correlated with body mass index (BMI) (P < 0.05, each). Age, smoking and BMI were independent predictors of urinary 8-OHdG excretion (P < 0.01, P < 0.01 and P < 0.05, respectively). In liver disease, the excretion of 8-OHdG was not changed, as compared with healthy individuals. However, the liver disease patients under the age of 40 had higher values of 8-OHdG than healthy persons. In addition, the urinary excretion of 8-OHdG was higher in patients with hepatitis C virus (HCV) infection than those with hepatitis B virus (HBV) infection.


CONCLUSIONS
The results of the present study suggest that measurement of urinary 8-OHdG excretion is useful in assessing DNA oxidation caused by aging, smoking, body composition and liver disease.
Ichiba
Miho
M
Division of Molecular and Genetic Medicine, Department of Gene and Regenerative Medicine, Graduate School of Medicine, Tottori University, Yonago 683-8504, Japan.
Yamada
Sadako
S
Ishii
Kyoko
K
Gonda
Kazue
K
Murai
Rie
R
Shimomura
Takashi
T
Saeki
Toshiya
T
Kanbe
Takamasa
T
Tanabe
Yoshitada
Y
Yoshida
Yoko
Y
Tsuchiya
Hiroyuki
H
Hoshikawa
Yoshiko
Y
Kurimasa
Akihiro
A
Kishimoto
Yukihiro
Y
Kawasaki
Hironaka
H
Shiota
Goshi
G
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D015992:Body Mass Index; D004247:DNA / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000652:urine*; D008297:Male; D008875:Middle Aged; D010100:Oxygen / Q000378:metabolism; D017382:Reactive Oxygen Species; D012016:Reference Values; D015203:Reproducibility of Results; D012907:Smoking
D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine; D010100:Oxygen