title: Lack of replication of genetic associations with human longevity.
abstract: The exceptional longevity of centenarians is due in part to inherited genetic factors, as deduced from data that show that first degree relatives of centenarians live longer and have reduced overall mortality. In recent years, a number of groups have performed genetic association studies on long-living individuals (LLI) and young controls to identify alleles that are either positively or negatively selected in the centenarian population as consequence of a demographic pressure. Many of the reported studies have shown genetic loci associated with longevity. Of these, with the exception of APOE, none have been convincingly reproduced. We validated our populations by typing the APOE locus. In addition, we used 749 American Caucasian LLI, organized in two independent tiers and 355 American Caucasian controls in the attempt to replicate previously published findings. We tested Klotho (KL)-VS variant (rs952706), Cholesteryl Ester Transfer Protein (CETP) I405V (rs5882), Paraoxonase 1 (PON1) Q192R (rs662), Apolipoprotein C-III (APOC3) -641C/A (rs2542052), Microsomal Transfer Protein (MTP) -493G/T (rs2866164) and apolipoprotein E (APOE) epsilon2 and epsilon4 isoforms, (rs7412 and rs429358) haplotypes respectively. Our results show that, at present, except for APOE, none of the selected genes show association with longevity if carefully tested in a large cohort of LLI and their controls, pointing to the need of larger populations for case-control studies in extreme longevity.
authors.affiliation: Genetic Department, IRCCS Multimedica, via Milanese 300, Sesto S. Giovanni (Milan), 20099, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Novelli
authors.forename: Chiara
authors.initials: C
authors.lastname: Viviani Anselmi
authors.forename: Roberta
authors.initials: R
authors.lastname: Roncarati
authors.forename: Guia
authors.initials: G
authors.lastname: Guffanti
authors.forename: Alberto
authors.initials: A
authors.lastname: Malovini
authors.forename: Giulio
authors.initials: G
authors.lastname: Piluso
authors.forename: Annibale Alessandro
authors.initials: AA
authors.lastname: Puca
mesh_terms: D000293:Adolescent; D000328:Adult; D000369:Aged, 80 and over; D001057:Apolipoproteins E / Q000235:genetics; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008136:Longevity / Q000235:genetics*; D008297:Male; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results; D018401:Sample Size; D014481:United States; D044465:White People / Q000235:genetics
chemical_list: D001057:Apolipoproteins E