Electron cryotomography.
Electron cryotomography is an emerging technique that allows the structures of unique biological objects such as individual macromolecules, viruses, and even small whole cells to be reconstructed in their near-native states in three dimensions (3-D) to an approximate 5-nm resolution. The required instrumentation, sample preparation and limitations, data collection, typical results, and future prospects are summarized briefly.
Murphy
Gavin E
GE
California Institute of Technology, Pasadena, CA, USA.
Jensen
Grant J
GJ
D001709:Biotechnology / Q000295:instrumentation / Q000379:methods / Q000639:trends; D020285:Cryoelectron Microscopy / Q000295:instrumentation / Q000379:methods* / Q000639:trends*; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods* / Q000639:trends*; D014054:Tomography / Q000295:instrumentation / Q000379:methods* / Q000639:trends*