title: Spectroscopic studies on a novel intramolecular hydrogen bond within the (6-4) photoproduct.
abstract: The (6-4) photoproduct, which is one of major UV-induced lesions formed between adjacent pyrimidine bases in DNA, was characterized by using (15)N NMR, fluorescence emission, and UV/VIS absorption at various pH values. From these experiments, we identified a novel intramolecular hydrogen bond between the hydroxyl group at the C5 position of the 5' component and the N3 of the 3' pyrimidone ring within the (6-4) photoproduct.
authors.affiliation: Division of Chemistry, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan. yamagen@chembio.chem.es.osaka-u.ac.jp
authors.forename: Junpei
authors.initials: J
authors.lastname: Yamamoto
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Kenichi
authors.initials: K
authors.lastname: Hitomi
authors.forename: Elizabeth D
authors.initials: ED
authors.lastname: Getzoff
authors.forename: Shigenori
authors.initials: S
authors.lastname: Iwai
mesh_terms: D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D019906:Nuclear Magnetic Resonance, Biomolecular; D011740:Pyrimidine Dimers / Q000737:chemistry*; D011741:Pyrimidine Nucleosides / Q000737:chemistry
chemical_list: C528345:5-methylpyrimidine-2-one 2'-deoxyribonucleoside; D011740:Pyrimidine Dimers; D011741:Pyrimidine Nucleosides; C058406:pyrimidine-pyrimidone dimer