Improvement of PCR for detection of Opisthorchis viverrini DNA in human stool samples.
Opisthorchis viverrini is an important food-borne trematode in Southeast Asia. The infection causes significant morbidity in terms of hepatobiliary diseases and cholangiocarcinoma. The aim of this study was to improve the sensitivity of the PCR-based diagnosis of O. viverrini infection. A new fecal DNA extraction protocol for the detection of O. viverrini DNA using cetyltrimethyl-ammoniumbromide to remove PCR inhibitor was used and compared with the commercial stool kit method. The sensitivity of the new test was 79.3%, compared with the 44.8% of the previous method (P < 0.01). PCR-positive tests identified several cases judged parasite negative by the parasitological method (28.6%), indicating the new test's advantage in the diagnosis of individuals with light infections.
Duenngai
Kunyarat
K
Department of Parasitology, Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Sithithaworn
Paiboon
P
Rudrappa
Umesha Kanasinakatte
UK
Iddya
Karunasagar
K
Laha
Thewarach
T
Stensvold
Christen Rune
CR
Strandgaard
Hanne
H
Johansen
Maria Vang
MV
D000818:Animals; D001210:Asia, Southeastern; D005243:Feces / Q000469:parasitology*; D006801:Humans; D009889:Opisthorchiasis / Q000175:diagnosis*; D009891:Opisthorchis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity