title: Detection of structural changes of RNA aptamer containing GGA repeats under the ionic condition using the microchip electrophoresis.
abstract: We performed in vitro selection against bovine prion protein (bPrP) using RNA pools with thirty-nucleotides randomized regions. Among the obtained RNA aptamers, RNA aptamer wt2 possessed four GGA triplets. It is known that RNA and DNA oligomers containing GGA repeat sequences show a G-quadruplex structure in the presence of K+ or Na+ ions using the NMR and crystallographic studies. Recently we have been studying RNA aptamer-protein, -peptide and -RNA interactions using microchip electrophoresis (ME). In this study, we used this system to detect structural changes of RNA aptamer wt2 in the presence of K+ and Na+ ions, and detected the dimer formation with simple operation.
authors.affiliation: Age Dimension Research Center, AIST, Tsukuba, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Nishikawa
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Murakami
authors.forename: Ken
authors.initials: K
authors.lastname: Noda
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nishikawa
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002417:Cattle; D019281:Dimerization; D046209:Electrophoresis, Microchip; D054856:G-Quadruplexes; D011188:Potassium / Q000737:chemistry; D011328:Prions / Q000737:chemistry; D012091:Repetitive Sequences, Nucleic Acid; D052156:SELEX Aptamer Technique; D012964:Sodium / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D011328:Prions; D012964:Sodium; D011188:Potassium