A phospholipase A1 platelet activator from the wasp venom of Vespa magnifica (Smith).
Wasp is an important venomous animal that can induce human fatalities. Aortic thrombosis and cerebral infarction are major clinical symptoms after massive wasp stings but the reason leading to the envenomation manifestation is still not known. In this paper, a toxin protein is purified and characterized by Sephadex G-75 gel filtration, CM-Sephadex C-25 cationic exchange and fast protein liquid chromatography (FPLC) from the venom of the wasp, Vespa magnifica (Smith). This protein, named magnifin, contains phospholipase-like activity and induces platelet aggregation. The cDNA encoding magnifin is cloned from the venom sac cDNA library of the wasp. The predicted protein was deduced from the cDNA with a sequence composed of 337 amino acid residues. Magnifin is very similar to other phospholipase A(1) (PLA(1)), especially to other wasp allergen PLA(1). Magnifin can activate platelet aggregation and induce thrombosis in vivo. The current results proved that PLA(1) in wasp venom could be contributable to aortic thrombosis after massive wasp stings.
Yang
Hailong
H
Biotoxin Units of Key Laboratories of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China.
Xu
Xueqing
X
Ma
Dongying
D
Zhang
Keyun
K
Lai
Ren
R
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D054466:Phospholipases A1 / Q000737:chemistry / Q000633:toxicity*; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits; D014862:Wasp Venoms / Q000737:chemistry / Q000633:toxicity*; D014863:Wasps
D018076:DNA, Complementary; D014862:Wasp Venoms; D054466:Phospholipases A1