title: Chiral separation of two pairs of enantiomers of tadalafil by high-performance liquid chromatography.
abstract: A high-performance liquid chromatographic method was developed for the chiral separation of a new selective phosphodiesterase 5 inhibitors, tadalafil and its three isomers. The chiral separation was performed on a Chiralpak AD column. The mobile phase was hexane-isopropyl alcohol (1:1, v/v). UV detection was at 220 nm. Baseline chiral separation for the four isomers was obtained within 30 min. Each of the resolutions of the two pairs enantiomers were more than 2.0. The limits of quantitation were 0.60, 0.90, 1.20, and 1.80 ng for (6R, 12aS), (6R, 12aR), (6S, 12aS), and (6S, 12aR) isomers, respectively. Relative standard deviation of the method was below 2% (n=5). The method is suitable in quality control.
authors.affiliation: College of Biological Science and Engineering, HeBei University of Science and Technology, Shijiazhuang 050018, China. wenhuigao@126.com
authors.forename: Wenhui
authors.initials: W
authors.lastname: Gao
authors.forename: Zhefeng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Li
authors.forename: Guijian
authors.initials: G
authors.lastname: Liang
mesh_terms: D002243:Carbolines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010726:Phosphodiesterase Inhibitors / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D000068581:Tadalafil
chemical_list: D002243:Carbolines; D010726:Phosphodiesterase Inhibitors; D000068581:Tadalafil