[Determination of tenuifolin in Tianwang Buxinwan and Guipiwan by HPLC].
OBJECTIVE
A quantitative method was developed for the determination of tenuifolin in Tianwang Buxinwan and Guipiwan by HPLC.


METHODS
The samples were separated by Alltima C18 column (4.6 mm x 250 mm, 5 microm) using methanol--0.05% phosphoric acid (65:35) as a mobile phase, flow rate was 1.0 mL x min(-1) and wavelength was set at 202 nm.


RESULTS
Tenuifolin was detected in both Chinese preparations. The number of theoretical plates calculated by tenuifolin peak was 2 500. The regression equation of tenuifolin was Y = 5.239 x 10(6) X-6.247 x 10(5) (r = 0.9994) and the liner range was 10-500 g x mL(-1). The average recovery of tenuifolin was 97.5% (RSD less than 3.0%). The LOD of tenuifolin was 5.50 g x mL(-1).


CONCLUSIONS
The method is sensitive, rapid and accurate.
Dong
Xiao-bing
XB
Institute of Chinese Medical Sciences of Macau University, Macau, China. dxbaa@ust.hk
Li
Jun
J
Jiang
Yong
Y
Zheng
Ying
Y
Dong
T X Tina
TX
Tu
Peng-fei
PF
Tsim
W K Karl
WK
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D029745:Codonopsis / Q000737:chemistry; D045786:Diterpenes, Kaurane / Q000032:analysis; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D031801:Polygala / Q000737:chemistry; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry; D012503:Saponins / Q000032:analysis; D014315:Triterpenes / Q000032:analysis
D045786:Diterpenes, Kaurane; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C532372:guipiwan; D012503:Saponins; C532370:tenuifolin diterpene; C532371:tianwang buxinwan; D014315:Triterpenes; C407311:tenuifolic saponin