Paclitaxel-octreotide conjugates inhibit growth of human non-small cell lung cancer cells in vitro.
OBJECTIVE
To evaluate the effects of paclitaxel-octreotide conjugates on the growth of cultured non-small cell lung cancer cells.


METHODS
RT-PCR was performed to detect mRNA for the subtypes of the human somatostatin receptor (SSTR) using specific primers. MTT-based cytotoxicity assay was used to evaluate the cell viability after treatment with paclitaxel and the conjugates. Cell cycle perturbations were determined using a Fluorescence-Activated Cell Sorter.


RESULTS
Non-small cell lung cancer A549 and Calu-6 cells expressed mRNA for SSTR2 and SSTR5. Paclitaxel and the conjugates effectively inhibited the growth of A549 and Calu-6 cells in a concentration- and time-dependent manner. In SSTR-negative fibroblasts, the conjugates were less cytotoxic than paclitaxel. The conjugates and paclitaxel could induce the increase of G(2)/M phase ratio in A549 cells.


CONCLUSIONS
The paclitaxel-octreotide conjugates can be used as selective-targeted chemotherapeutic agents for treating non-small cell lung cancer.
Sun
M L
ML
Department of Oncology, Qilu Hospital, Shandong University, Jinan, 250012, China.
Wei
J M
JM
Wang
X W
XW
Li
L
L
Wang
P
P
Li
M
M
Yi
C H
CH
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D017931:DNA Primers; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D008175:Lung Neoplasms / Q000188:drug therapy*; D015282:Octreotide / Q000008:administration & dosage*; D017239:Paclitaxel / Q000008:administration & dosage*; D012333:RNA, Messenger / Q000032:analysis; D017481:Receptors, Somatostatin / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000970:Antineoplastic Agents; D017931:DNA Primers; D012333:RNA, Messenger; D017481:Receptors, Somatostatin; D017239:Paclitaxel; D015282:Octreotide