title: Polyethylene glycol (PEG)-mediated transient gene expression in a red alga, Cyanidioschyzon merolae 10D.
abstract: DNA introduction into cells is an essential technique for molecular genetic analysis. Here, we show that DNA is easily introduced into cells of the unicellular red alga Cyanidioschyzon merolae by a polyethylene glycol (PEG)-mediated protocol. In this study, the beta-tubulin gene of C. merolae was cloned on a plasmid and a hemagglutinin (HA) tag then added at the C-terminus. This plasmid was then introduced into C. merolae cells by a PEG-mediated transformation protocol. At 24 h after PEG-mediated transformation, intracellular localization of the tagged protein was detected by anti-HA immunocytochemistry, indicating the utility of this transient expression system for molecular genetic analyses.
authors.affiliation: Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-0032 Japan.
authors.forename: Mio
authors.initials: M
authors.lastname: Ohnuma
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Takayuki
authors.initials: T
authors.lastname: Inouye
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Sekine
authors.forename: Kan
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011092:Polyethylene Glycols; D000461:Rhodophyta / Q000235:genetics*; D014162:Transfection / Q000379:methods*
chemical_list: D011092:Polyethylene Glycols; D004247:DNA