title: CD8+ T cells are associated with severe gastritis in Helicobacter pylori-infected mice in the absence of CD4+ T cells.
abstract: Helicobacter pylori infection results in the development of chronic gastritis, and CD4+ T cells are a major component of the gastric cellular infiltrate. To examine whether CD4+ T cells are important in initiating and maintaining H. pylori-induced gastritis, mice deficient in CD4+ T cells (B6.BM1.GK 1.5 mice [GK 1.5 mice]) were infected with H. pylori. We found that as in normal mice, H. pylori-specific antibodies, mostly of the immunoglobulin M isotype, developed in GK 1.5 mice but were unable to cure H. pylori infection. Further, while the stomachs of H. pylori-infected GK 1.5 mice were more heavily infiltrated with CD8+ T cells and B cells, mice deficient in both CD4+ and CD8+ T cells developed mild inflammation comparable to the level observed for C57BL/6 mice. These observations suggest that CD4+ T cells may play an important role in regulating or suppressing gastric CD8+ T cells which, in the absence of CD4+ T cells, may mediate more-severe disease. These studies have revealed a potentially important role for CD8+ T cells in the gastric disease resulting from H. pylori infection.
authors.affiliation: Department of Microbiology and Immunology and The Australian Bacterial Pathogenesis Program, University of Melbourne, Melbourne, Victoria 3010, Australia.
authors.forename: Mai Ping
authors.initials: MP
authors.lastname: Tan
authors.forename: John
authors.initials: J
authors.lastname: Pedersen
authors.forename: Yifan
authors.initials: Y
authors.lastname: Zhan
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Lew
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Pearse
authors.forename: Odilia L C
authors.initials: OL
authors.lastname: Wijburg
authors.forename: Richard A
authors.initials: RA
authors.lastname: Strugnell
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001402:B-Lymphocytes / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D015169:Colony Count, Microbial; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D005753:Gastric Mucosa / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000276:immunology / Q000382:microbiology* / Q000473:pathology*; D016481:Helicobacter Infections / Q000276:immunology* / Q000382:microbiology / Q000473:pathology*; D016480:Helicobacter pylori / Q000276:immunology*; D007075:Immunoglobulin M / Q000097:blood; D016131:Lymphocyte Subsets / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
chemical_list: D000907:Antibodies, Bacterial; D007075:Immunoglobulin M