title: Improvement of PCR for detection of Opisthorchis viverrini DNA in human stool samples.
abstract: Opisthorchis viverrini is an important food-borne trematode in Southeast Asia. The infection causes significant morbidity in terms of hepatobiliary diseases and cholangiocarcinoma. The aim of this study was to improve the sensitivity of the PCR-based diagnosis of O. viverrini infection. A new fecal DNA extraction protocol for the detection of O. viverrini DNA using cetyltrimethyl-ammoniumbromide to remove PCR inhibitor was used and compared with the commercial stool kit method. The sensitivity of the new test was 79.3%, compared with the 44.8% of the previous method (P < 0.01). PCR-positive tests identified several cases judged parasite negative by the parasitological method (28.6%), indicating the new test's advantage in the diagnosis of individuals with light infections.
authors.affiliation: Department of Parasitology, Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Kunyarat
authors.initials: K
authors.lastname: Duenngai
authors.forename: Paiboon
authors.initials: P
authors.lastname: Sithithaworn
authors.forename: Umesha Kanasinakatte
authors.initials: UK
authors.lastname: Rudrappa
authors.forename: Karunasagar
authors.initials: K
authors.lastname: Iddya
authors.forename: Thewarach
authors.initials: T
authors.lastname: Laha
authors.forename: Christen Rune
authors.initials: CR
authors.lastname: Stensvold
authors.forename: Hanne
authors.initials: H
authors.lastname: Strandgaard
authors.forename: Maria Vang
authors.initials: MV
authors.lastname: Johansen
mesh_terms: D000818:Animals; D001210:Asia, Southeastern; D005243:Feces / Q000469:parasitology*; D006801:Humans; D009889:Opisthorchiasis / Q000175:diagnosis*; D009891:Opisthorchis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity