Rebuilding host-pathogen interaction from the ground up: in vitro reconstitution of the inflammasome.
The inflammasome is a macromolecular complex responsible for the proteolytic processing and activation of the secreted cytokine IL-1beta. It is assembled and activated in response to upstream intracellular sensors of microbial components and cell injury. Now, Faustin et al. describe an in vitro cell-free reconstitution of the inflammasome, opening up a new avenue to better understand this innate immune pathway of microbe sensing.
Duncan
Joseph A
JA
Department of Medicine, Division of Infectious Diseases, University of North Carolina at Chapel Hill, UNC-CH, Chapel Hill, NC 27599-7295, USA. joseph_duncan@med.unc.edu
Ting
Jenny Pan-Yun
JP
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D004789:Enzyme Activation; D006790:Host-Parasite Interactions; D006801:Humans; D007113:Immunity, Innate / Q000502:physiology*; D053583:Interleukin-1beta / Q000276:immunology*; D046911:Macromolecular Substances / Q000378:metabolism*; D000070576:NLR Proteins; D015398:Signal Transduction / Q000502:physiology
D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D053583:Interleukin-1beta; D046911:Macromolecular Substances; D000070576:NLR Proteins; C427903:NLRP1 protein, human