Crystallization and preliminary crystallographic analysis of an octaketide-producing plant type III polyketide synthase.
Octaketide synthase (OKS) from Aloe arborescens is a plant-specific type III polyketide synthase that produces SEK4 and SEK4b from eight molecules of malonyl-CoA. Recombinant OKS expressed in Escherichia coli was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group I422, with unit-cell parameters a = b = 110.2, c = 281.4 A, alpha = beta = gamma = 90.0 degrees . Diffraction data were collected to 2.6 A resolution using synchrotron radiation at BL24XU of SPring-8.
Morita
Hiroyuki
H
Mitsubishi Kagaku Institute of Life Sciences (MITILS), 11 Minamiooya, Machida, Tokyo 194-8511, Japan.
Kondo
Shin
S
Kato
Ryohei
R
Wanibuchi
Kiyofumi
K
Noguchi
Hiroshi
H
Sugio
Shigetoshi
S
Abe
Ikuro
I
Kohno
Toshiyuki
T
D000504:Aloe / Q000201:enzymology; D003460:Crystallization; D018360:Crystallography, X-Ray; D048630:Polyketide Synthases / Q000737:chemistry*
D048630:Polyketide Synthases