title: [Determination of chyodeoxycholic acid in Bile Arisaema by HPLC-ELSD].
abstract: OBJECTIVE
  To develop an HPLC method for the determination of hyodeoxycholic acid in Bile Arisaema.
  
  
  METHODS
  The methanol extracts were separated on a Diamonsil ODS column eluted at 35 degrees C with a mixture of methanol-water-glacial acetic acid (75:25:0.01) at flow-rate 1.0 mL x min(-1). The drift-tube temperature of ELSD was 85 degrees C, and the flow rate of air was 3.0 L x min(-1).
  
  
  RESULTS
  Hyodeoxycholic acid in methanol extract was well separated, the relationship of logarithms of injection amount and peak area was linear (r = 0.9998) over the range of 2.4-24 microg. The average recovery was 97.1% (RSD 2.9%, n=5).
  
  
  CONCLUSIONS
  The method could be used for quality control of Bile Arisaema.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.forename: Yong-xin
authors.initials: YX
authors.lastname: Zhang
authors.forename: Hui-dong
authors.initials: HD
authors.lastname: Zhao
authors.forename: Qi-wei
authors.initials: QW
authors.lastname: Zhang
mesh_terms: D000818:Animals; D031057:Arisaema / Q000737:chemistry*; D001646:Bile / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003840:Deoxycholic Acid / Q000032:analysis*; D004338:Drug Combinations; D006358:Hot Temperature; D008027:Light; D008516:Medicine, Chinese Traditional / Q000592:standards; D035281:Plant Tubers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012542:Scattering, Radiation
chemical_list: D004338:Drug Combinations; D003840:Deoxycholic Acid; C010471:hyodeoxycholic acid