title: Adenosine receptor-mediated adhesion of endothelial progenitors to cardiac microvascular endothelial cells.
abstract: Intracoronary delivery of endothelial progenitor cells (EPCs) is an emerging concept for the treatment of cardiovascular disease. Enhancement of EPC adhesion to vascular endothelium could improve cell retention within targeted organs. Because extracellular adenosine is elevated at sites of ischemia and stimulates neovascularization, we examined the potential role of adenosine in augmenting EPC retention to cardiac microvascular endothelium. Stimulation of adenosine receptors in murine embryonic EPCs (eEPCs) and cardiac endothelial cells (cECs) rapidly, within minutes, increased eEPC adhesion to cECs under static and flow conditions. Similarly, adhesion of human adult culture-expanded EPCs to human cECs was increased by stimulation of adenosine receptors. Furthermore, adenosine increased eEPC retention in isolated mouse hearts perfused with eEPCs. We determined that eEPCs and cECs preferentially express functional A1 and A2B adenosine receptor subtypes, respectively, and that both subtypes are involved in the regulation of eEPC adhesion to cECs. We documented that the interaction between P-selectin and its ligand (P-selectin glycoprotein ligand-1) plays a role in adenosine-dependent eEPC adhesion to cECs and that stimulation of adenosine receptors in cECs induces rapid cell surface expression of P-selectin. Our results suggest a role for adenosine in vasculogenesis and its potential use to stimulate engraftment in cell-based therapies.
authors.affiliation: Department of Medicine, Vanderbilt University, Nashville, Tenn, USA.
authors.forename: Sergey
authors.initials: S
authors.lastname: Ryzhov
authors.forename: Nataliya V
authors.initials: NV
authors.lastname: Solenkova
authors.forename: Anna E
authors.initials: AE
authors.lastname: Goldstein
authors.forename: Mathias
authors.initials: M
authors.lastname: Lamparter
authors.forename: Todd
authors.initials: T
authors.lastname: Fleenor
authors.forename: Pampee P
authors.initials: PP
authors.lastname: Young
authors.forename: James P
authors.initials: JP
authors.lastname: Greelish
authors.forename: John G
authors.initials: JG
authors.lastname: Byrne
authors.forename: Douglas E
authors.initials: DE
authors.lastname: Vaughan
authors.forename: Italo
authors.initials: I
authors.lastname: Biaggioni
authors.forename: Antonis K
authors.initials: AK
authors.lastname: Hatzopoulos
authors.forename: Igor
authors.initials: I
authors.lastname: Feoktistov
mesh_terms: D000241:Adenosine / Q000378:metabolism / Q000494:pharmacology*; D058907:Adenosine A1 Receptor Agonists; D058908:Adenosine A2 Receptor Agonists; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D017202:Myocardial Ischemia / Q000378:metabolism / Q000628:therapy; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology*; D019007:P-Selectin / Q000096:biosynthesis; D043682:Receptor, Adenosine A1 / Q000096:biosynthesis*; D043704:Receptor, Adenosine A2B / Q000096:biosynthesis*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D014665:Vasodilator Agents / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D058907:Adenosine A1 Receptor Agonists; D058908:Adenosine A2 Receptor Agonists; D008562:Membrane Glycoproteins; D019007:P-Selectin; C084882:P-selectin ligand protein; D043682:Receptor, Adenosine A1; D043704:Receptor, Adenosine A2B; D014665:Vasodilator Agents; D000241:Adenosine