Control of the molecular degradation of hyaluronic acid hydrogels for tissue augmentation.
A novel protocol to control the molecular degradation of hyaluronic acid (HA) hydrogels was successfully developed for tissue augmentation applications. HA has a different conformational structure in water and organic solvent, and the carboxyl group of HA is known to be the recognition site of hyaluronidase and HA receptors. Based on these findings, HA was chemically modified by grafting adipic acid dihydrazide (ADH) to the carboxyl group of HA in the water to prepare HA-ADH(WATER) and in the mixed solvent of water and ethanol to prepare degradation-controlled HA-ADH(WATER/ETHANOL). Three kinds of HA hydrogels were prepared by the crosslinking of HA-ADH(WATER) or HA-ADH(WATER/ETHANOL) with bis(sulfosuccinimidyl) suberate, and by the crosslinking of HA-OH with divinyl sulfone (DVS). In vitro and in vivo degradation tests showed that HA-DVS hydrogels were degraded most rapidly, followed by HA-ADH(WATER) hydrogels and HA-ADH(WATER/ETHANOL) hydrogels. There was no adverse effect during and after in vivo degradation tests. All of the HA hydrogel samples appeared to be biocompatible, according to the histological analysis with hematoxylin-eosin and Alcian blue.
Oh
Eun Ju
EJ
Department of Material Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Kyungbuk 790-784, Korea.
Kang
Sun-Woong
SW
Kim
Byung-Soo
BS
Jiang
Ge
G
Cho
Il Hwan
IH
Hahn
Sei Kwang
SK
D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000378:metabolism*; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D020100:Hydrogels / Q000378:metabolism*; D007279:Injections, Subcutaneous; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008422:Materials Testing; D051379:Mice; D008813:Mice, Inbred ICR; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D020100:Hydrogels; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase