Oligonucleotide array comparative genomic hybridization.
The array CGH technique (array comparative genome hybridization) has been developed to detect chromosomal copy number changes on a genome-wide and/or high-resolution scale. Here, we present validated protocols using in-house spotted oligonucleotide libraries for array CGH. This oligo array CGH platform yields reproducible results and is capable of detecting single copy gains, multicopy amplifications as well as homozygous and heterozygous deletions with high resolution. The protocol allows as little as 300 ng of input DNA, which makes the procedure valuable for small clinical samples and is also functional for DNA samples obtained from archival tissues.
Ijssel
Paul van den
Pv
Ylstra
Bauke
B
D045744:Cell Line, Tumor; D004247:DNA / Q000235:genetics; D023281:Genomics; D006801:Humans; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis
D004247:DNA