An Arabidopsis thaliana high-affinity molybdate transporter required for efficient uptake of molybdate from soil.
Molybdenum (Mo) is a trace element essential for living organisms, however no molybdate transporter has been identified in eukaryotes. Here, we report the identification of a molybdate transporter, MOT1, from Arabidopsis thaliana. MOT1 is expressed in both roots and shoots, and the MOT1 protein is localized, in part, to plasma membranes and to vesicles. MOT1 is required for efficient uptake and translocation of molybdate and for normal growth under conditions of limited molybdate supply. Kinetics studies in yeast revealed that the K(m) value of MOT1 for molybdate is approximately 20 nM. Furthermore, Mo uptake by MOT1 in yeast was not affected by coexistent sulfate, and MOT1 did not complement a sulfate transporter-deficient yeast mutant strain. These data confirmed that MOT1 is specific for molybdate and that the high affinity of MOT1 allows plants to obtain scarce Mo from soil.
Tomatsu
Hajime
H
Biotechnology Research Center and Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Takano
Junpei
J
Takahashi
Hideki
H
Watanabe-Takahashi
Akiko
A
Shibagaki
Nakako
N
Fujiwara
Toru
T
D027321:Anion Transport Proteins / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D004269:DNA, Bacterial / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D008982:Molybdenum / Q000378:metabolism*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D012987:Soil
D027321:Anion Transport Proteins; D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; C525371:MOT1 protein, Arabidopsis; D012987:Soil; C036483:T-DNA; C044659:molybdate; D008982:Molybdenum