Functional analysis of the role of Fur in the virulence of Pseudomonas syringae pv. tabaci 11528: Fur controls expression of genes involved in quorum-sensing.
In most bacteria, Fur (ferric uptake regulator) is a crucial global regulator known to operate not only in the regulation of iron homeostasis but also in a variety of other cellular processes. In an effort to characterize the role of Fur in the virulence of plant pathogens, a fur homolog was isolated from Pseudomonas syringae pv. tabaci 11528. Phenotype assays showed that a fur deletion mutant (BL33) constitutively produced siderophore(s) and exhibited decreases in swarming motility as well as the synthesis of tabtoxin and N-acyl homoserine lactones. Consistent with the results of TLC, quantitative real-time RT-PCR of the QS associated genes psyR and psyI demonstrated that Fur up-regulates these genes at the transcriptional level. Finally, the effects of a fur mutation on plant virulence indicated that Fur-regulated traits are relevant to plant-pathogen interactions.
Cha
Ji Young
JY
Department of Microbiology, College of Natural Science, Pusan National University, Busan 609-735, Republic of Korea.
Lee
Jun Seung
JS
Oh
Jeong-Il
JI
Choi
Jang Won
JW
Baik
Hyung Suk
HS
D001426:Bacterial Proteins / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D044224:Pseudomonas syringae / Q000472:pathogenicity* / Q000502:physiology*; D053038:Quorum Sensing / Q000502:physiology*; D012097:Repressor Proteins / Q000378:metabolism*; D014026:Nicotiana / Q000382:microbiology* / Q000502:physiology*; D014774:Virulence
D001426:Bacterial Proteins; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial