Expression of mast cell tryptases in Hodgkin and Reed-Sternberg (HRS) cells.
Tryptase is the most abundant protease in human mast cells, and is often used as a marker for the enumeration of mast cells in tissue. Here we report that tumour cells from Hodgkin lymphoma, the so called Hodgkin and Reed-Sternberg cells, can express tryptase. Hodgkin lymphoma cell lines expressed mRNA for both alpha- and beta-tryptase and also produced the protein, although at much lower concentrations than mast cells. However, the frequency of tryptase positive HRS-cells in situ was very low. This report demonstrates that tumour cells of lymphoid origin can express tryptase in vitro and in situ.
Carvalho
Ricardo F S
RF
Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
Mahshid
Yilmaz
Y
Claesson
Hans-Erik
HE
Glimelius
Ingrid
I
Fischer
Marie
M
Nilsson
Gunnar
G
D045744:Cell Line, Tumor; D006689:Hodgkin Disease / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008407:Mast Cells / Q000201:enzymology*; D016539:Reed-Sternberg Cells / Q000201:enzymology* / Q000473:pathology; D053802:Tryptases / Q000096:biosynthesis / Q000235:genetics*; D014407:Tumor Cells, Cultured
D007527:Isoenzymes; D053802:Tryptases