title: Isolation of various Arcobacter species from domestic geese (Anser anser).
abstract: In this study, the prevalence and distribution of various Arcobacter spp. were investigated in samples taken from the cloacae of healthy domestic geese raised in Turkey. A membrane filtration technique with a non-selective blood agar was employed after enrichment in Arcobacter enrichment broth (AEB) to isolate a wide range of Arcobacter spp. In addition, the isolates were characterized phenotypically and identified at species level using a multiplex-PCR assay. A total of 90 cloacal swab samples taken from geese, collected on three farms (18, 25, 47 samples, respectively), were examined. Of the samples examined, 16 (18%) were found positive for Arcobacter. One Arcobacter species was isolated from each bird. Of the 16 Arcobacter isolates, 7 (44%), 7 (44%) and 2 (12.5%) were identified by m-PCR as A. cryaerophilus, A. skirrowii and A. butzleri, respectively. The present study indicates that domestic geese can harbour a variety of Arcobacter spp. in their cloacae. The presence of Arcobacter in geese may be of significance as reservoirs in their dissemination. Detailed research is needed for better understanding of the epidemiology and zoonotic potential of this emerging pathogen.
authors.affiliation: Department of Food Engineering, Faculty of Engineering, Izmir Institute of Technology, Urla, 35430 Izmir, Turkey. halilatabay@iyte.edu.tr
authors.forename: H Ibrahim
authors.initials: HI
authors.lastname: Atabay
authors.forename: Ahmet
authors.initials: A
authors.lastname: Unver
authors.forename: Mitat
authors.initials: M
authors.lastname: Sahin
authors.forename: Salih
authors.initials: S
authors.lastname: Otlu
authors.forename: Mehmet
authors.initials: M
authors.lastname: Elmali
authors.forename: Hilmi
authors.initials: H
authors.lastname: Yaman
mesh_terms: D000818:Animals; D020640:Arcobacter / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification*; D002988:Cloaca / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004197:Disease Reservoirs / Q000382:microbiology / Q000662:veterinary; D005777:Geese; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011201:Poultry Diseases / Q000175:diagnosis / Q000382:microbiology*; D015995:Prevalence; D013045:Species Specificity; D014421:Turkey / Q000453:epidemiology
chemical_list: D004269:DNA, Bacterial