title: Isolation and characterization of some mycelia inhabiting Tuber ascomata.
abstract: Tuber spp. are ectomycorrhizal ascomycetes that produce subterranean ascomata known as truffles. Truffles can be regarded as complex microhabitats hosting bacteria and yeasts. In this paper we show that guest filamentous fungi are also associated to truffle ascomata, regardless of the Tuber spp., and report the morpho-molecular characterization of seven truffle-hosted mycelia isolated from healthy and intact Tuber ascomata. Some of these isolates were shown to be related to the fungal endophytes of plants. Interestingly, the truffle-hosted mycelia grew stuck to the hyphal wall of their partner when co-cultivated with the Tuber borchii mycelium, but not when co-cultivated with the test species Agaricus macrosporus. The present data suggest that guest filamentous fungi can be added to the list of truffle-interacting microorganisms.
authors.affiliation: Department of Environmental Sciences, University of L'Aquila-Italy, Via Vetoio Loc. Coppito, I-67010 L'Aquila, Italy. pacioni@univaq.it
authors.forename: Giovanni
authors.initials: G
authors.lastname: Pacioni
authors.forename: Marco
authors.initials: M
authors.lastname: Leonardi
authors.forename: Pierpaolo
authors.initials: P
authors.lastname: Aimola
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Ragnelli
authors.forename: Andrea
authors.initials: A
authors.lastname: Rubini
authors.forename: Francesco
authors.initials: F
authors.lastname: Paolocci
mesh_terms: D001203:Ascomycota / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure; D001487:Basidiomycota / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure; D003470:Culture Media; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D025282:Mycelium / Q000254:growth & development* / Q000648:ultrastructure; D017422:Sequence Analysis, DNA
chemical_list: D003470:Culture Media; D004271:DNA, Fungal