A loop resonator for slice-selective in vivo EPR imaging in rats.
A loop resonator was developed for 300 MHz continuous-wave electron paramagnetic resonance (CW-EPR) spectroscopy and imaging in live rats. A single-turn loop (55 mm in diameter) was used to provide sufficient space for the rat body. Efficiency for generating a radiofrequency magnetic field of 38 microT/W(1/2) was achieved at the center of the loop. For the resonator itself, an unloaded quality factor of 430 was obtained. When a 350 g rat was placed in the resonator at the level of the lower abdomen, the quality factor decreased to 18. The sensitive volume in the loop was visualized with a bottle filled with an aqueous solution of the nitroxide spin probe 3-carbamoyl-2,2,5,5-tetramethyl-3-pyrrolin-1-yloxy (3-CP). The resonator was shown to enable EPR imaging in live rats. Imaging was performed for 3-CP that had been infused intravenously into the rat and its distribution was visualized within the lower abdomen.
Hirata
Hiroshi
H
Department of Electrical Engineering, Yamagata University, Yonezawa, Yamagata 992-8510, Japan. hhirata@yz.yamagata-u.ac.jp
He
Guanglong
G
Deng
Yuanmu
Y
Salikhov
Ildar
I
Petryakov
Sergey
S
Zweier
Jay L
JL
D000818:Animals; D003497:Cyclic N-Oxides; D017352:Echo-Planar Imaging / Q000295:instrumentation*; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted; D008297:Male; D019047:Phantoms, Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D013113:Spin Labels
D003497:Cyclic N-Oxides; D013113:Spin Labels; C047253:3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-1-yloxyl