title: Chromosome variability of human multipotent mesenchymal stromal cells.
abstract: We elaborated a method of preparing cytogenetic preparations of cultured multipotent mesenchymal stromal cells from the adipose tissue. It was found that karyotypic changes (monosomy, translocations) appear in some samples during culturing. Clones with changed karyotype were detected in 11-14-passage cultures from 2 of 7 individuals. The percent of aberrant cells in cultures from different individuals varied from 1.5 to 5.95 per 100 cells, which attested to karyotype instability. These data substantiate the need for cytogenetic control of cells before their transplantation into donor organism and further investigation of chromosome variability in stem cells.
authors.affiliation: I. M. Setchenov Moscow Medical Academy.
authors.forename: N P
authors.initials: NP
authors.lastname: Bochkov
authors.forename: E S
authors.initials: ES
authors.lastname: Voronina
authors.forename: N V
authors.initials: NV
authors.lastname: Kosyakova
authors.forename: T
authors.initials: T
authors.lastname: Liehr
authors.forename: A A
authors.initials: AA
authors.lastname: Rzhaninova
authors.forename: L D
authors.initials: LD
authors.lastname: Katosova
authors.forename: V I
authors.initials: VI
authors.lastname: Platonova
authors.forename: D V
authors.initials: DV
authors.lastname: Gol'dshtein
mesh_terms: D000328:Adult; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002877:Chromosomes, Human; D020732:Cytogenetic Analysis; D006801:Humans; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology