Synthesis of glutathione C60 derivative and its protective effect on hydrogen peroxide-induced apoptosis in rat pheochromocytoma cells.
Oxidized glutathione C(60) derivative has been synthesized and characterized in our research. As a novel derivative of C(60), the glutathione C(60) derivative is soluble in dimethylsulfoxide, dimethylformamide and dimethylacetamide. Rat pheochromocytoma (PC12) cells are treated with hydrogen peroxide and underwent cytotoxicity; apoptotic death is determined by MTT assay, flow cytometry analysis, PI/Hoechst 33342 staining and glutathione assay. The results suggest that glutathione C(60) derivative has the potential to prevent oxidative stress-induced cell death without evident toxicity.
Hu
Zhen
Z
Department of Chemistry, Huazhong University of Science and Technology, Wuhan 430074, China.
Liu
Shenghong
S
Wei
Yen
Y
Tong
Etang
E
Cao
Fei
F
Guan
Wenchao
W
D000818:Animals; D000975:Antioxidants / Q000138:chemical synthesis / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D001562:Benzimidazoles / Q000737:chemistry; D001681:Biological Assay; D003603:Cytotoxins / Q000037:antagonists & inhibitors / Q000633:toxicity; D019281:Dimerization; D005434:Flow Cytometry; D037741:Fullerenes / Q000737:chemistry / Q000494:pharmacology*; D005978:Glutathione / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity; D015394:Molecular Structure; D009410:Nerve Degeneration / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018696:Neuroprotective Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D016716:PC12 Cells; D051381:Rats; D013778:Tetrazolium Salts / Q000737:chemistry
D000975:Antioxidants; D001562:Benzimidazoles; D003603:Cytotoxins; D037741:Fullerenes; D018696:Neuroprotective Agents; D013778:Tetrazolium Salts; C526952:glutathione C(60)-fullerene complex; D006861:Hydrogen Peroxide; D005978:Glutathione; C069837:fullerene C60; C017807:bisbenzimide ethoxide trihydrochloride