title: Interactions with RNA/DNA of proteins involved in the regulation of transcription, translation and telomere elongation.
abstract: Interactions with DNA and RNA of three different proteins involved in the regulation of (1) transcription, (2) translation, and (3) telomere elongation were examined by NMR. In the first case, the combination of structural determination, dynamical analysis on the basis of relaxation data and identification of interactive surface for wild and phosphorylation-mimicking mutant proteins has given the insight on the increase of DNA-binding affinity through phosphorylation of the protein. In the second case, the arrangement of two tandem domains interacting with RNA has been determined with residual dipolar couplings and paramagnetic relaxation enhancement, which has given the idea on how the two tandem domains recognize the target RNA. In the third case, simultaneous binding of the other two tandem domains to both DNA and RNA has been analyzed with chemical shift perturbation analysis. The result has suggested that the protein composed of two tandem domains can recruit telomerase to telomere DNA.
authors.affiliation: Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Ohyama
authors.forename: Ayako
authors.initials: A
authors.lastname: Furukawa
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Miyoshi
authors.forename: Yuusuke
authors.initials: Y
authors.lastname: Takada
authors.forename: Shouta
authors.initials: S
authors.lastname: Ohgara
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hiratsuka
authors.forename: Takao
authors.initials: T
authors.lastname: Imai
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nakagama
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagata
authors.forename: Masato
authors.initials: M
authors.lastname: Katahira
mesh_terms: D004247:DNA / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry*; D005786:Gene Expression Regulation; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D012313:RNA / Q000737:chemistry; D016601:RNA-Binding Proteins / Q000737:chemistry*; D016615:Telomere / Q000737:chemistry; D014157:Transcription Factors / Q000737:chemistry*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; C077926:GT-1 transcription factor; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D016601:RNA-Binding Proteins; D014157:Transcription Factors; D012313:RNA; D004247:DNA