title: [Observation of pollen tube germination of Platycodon grandiflorum by fluorescence microscopy].
abstract: OBJECTIVE
  To determine the self-compatibility of Platycodon grandiflorum and the location of microspore germination on stigma.
  
  
  METHODS
  The microspore germination of self-pollination, self-plant-pollination and cross-pollination and the pollination microspore germination in and outside of stigma were observed with fluorescence microscopy.
  
  
  RESULTS
  Most pollens of self-pollination, self-plant-pollination and cross-pollination can germinate on the stigma, and after 24 hours, pollen tube entered into the ovary successfully. Pollinated on the outer-surface of stigma, microspores could not germinate, but on the inner-surface of the stigma when it dehisced most microspores can germinate.
  
  
  CONCLUSIONS
  The compatibility of self-plant-pollination of Platycodon grandiflorum is high. The microspore germination loci is on the inner-surface of the dehisced stigma.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Chinese Peking Union Medical College, Beijing 100094, China.
authors.forename: Jian-he
authors.initials: JH
authors.lastname: Wei
authors.forename: Feng-jie
authors.initials: FJ
authors.lastname: Ma
authors.forename: Lu-qi
authors.initials: LQ
authors.lastname: Huang
authors.forename: Cheng-min
authors.initials: CM
authors.lastname: Yang
authors.forename: Zhi-tu
authors.initials: ZT
authors.lastname: Sun
authors.forename: Qi-ting
authors.initials: QT
authors.lastname: Dong
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Cai
mesh_terms: D035264:Flowers / Q000254:growth & development; D008856:Microscopy, Fluorescence / Q000379:methods; D010946:Plants, Medicinal / Q000254:growth & development*; D031238:Platycodon / Q000254:growth & development*; D053205:Pollen Tube / Q000254:growth & development*; D054817:Pollination / Q000502:physiology