Relationship between adenovirus DNA replication proteins and nucleolar proteins B23.1 and B23.2.
Adenovirus infection subverts nucleolar structure and function. B23 is a nucleolar protein present in two isoforms (B23.1 and B23.2) and both isoforms have been identified as stimulatory factors for adenovirus DNA replication. Here, it is demonstrated that the two isoforms of B23, B23.1 and B23.2, interact and co-localize differently with viral DNA replication proteins pTP and DBP in adenovirus-infected cells. Thus, the mechanism by which the two proteins stimulate viral DNA replication is likely to differ. These data also demonstrate the importance of testing both isoforms of B23 for interactions with viral proteins and nucleic acids.
Hindley
Clemence E
CE
Department of Cellular and Molecular Medicine, University Walk, University of Bristol, Bristol BS8 1TD, UK.
Davidson
Andrew D
AD
Department of Cellular and Molecular Medicine, University Walk, University of Bristol, Bristol BS8 1TD, UK.
Matthews
David A
DA
Department of Cellular and Molecular Medicine, University Walk, University of Bristol, Bristol BS8 1TD, UK.
D000256:Adenoviridae / Q000502:physiology*; D000257:Adenoviridae Infections / Q000378:metabolism* / Q000821:virology; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
D004279:DNA, Viral; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D014764:Viral Proteins; D004259:DNA-Directed DNA Polymerase