Centrifugal forces within usually-used magnitude elicited a transitory and reversible change in proliferation and gene expression of osteoblastic cells UMR-106.
Centrifugation is an important step in biochemical and molecular biological researches. But the effects of centrifugal stress on cells are still unclear. In this study, osteoblastic cells UMR-106 were subjected to a moderate centrifugal stress at 209 x g for 10 min. Then the cell proliferation and gene transcription after centrifugation were analyzed with flow cytometry and Real-time RT-PCR techniques, respectively. The result showed that the cell proliferation and mRNA expression of Runx2/Cbfa1, Collagen I and osteocalcin changed shortly after centrifugal loading, but recovered to pre-load levels within 24 h. A dose-response study of exposure cells to centrifugal force at 209, 253 and 301 x g showed that the centrifugal forces within usually-used range can rapidly influenced the mRNA expression of the osteoblast-specific genes, but no statistical differences were found among the three centrifugal magnitudes. And the fast regulation in the investigated genes was proved to be related to increased c-fos mRNA levels and subsequent activation of RTK and integrity of cytoskeleton construction. The result showed that the osteoblastic cells displayed a fast auto-regulation to usually-used centrifugal stress through multiple signal pathways.
Li
Juan
J
Department of Orthodontics, West China College of Stomatology, Sichuan University, 14#, 3rd Section, Renmin South Road, Chengdu 610041, P.R. China.
Jiang
Lingyong
L
Liao
Ga
G
Chen
Guoping
G
Liu
Ying
Y
Wang
Jun
J
Zheng
Yi
Y
Luo
Songjiao
S
Zhao
Zhihe
Z
D002460:Cell Line; D049109:Cell Proliferation; D002498:Centrifugation; D005786:Gene Expression Regulation; D016762:Genes, fos; D006801:Humans; D010006:Osteoblasts / Q000166:cytology*; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction; D013314:Stress, Mechanical; D014158:Transcription, Genetic
D012333:RNA, Messenger