[In vivo study on influence of a discrete nano-hydroxyapatite on leukemia P388 tissue in BALB/C mice].
OBJECTIVE
To study the influence of a discrete nano-hydroxyapatite crystal (nano-HAp) on lymphatic leukemia P388 behavior by in vivo techniques.


METHODS
A nano-HAp was prepared by a neutralization reaction of 0.1 mol calcium hydroxide suspension and 0.06 mol phosphoric acid solutions at room temperature over pH7. The various doses of the nano-HAp only and the nano-HAp mixture with cyclophosphamide (CY) were injected into mice inoculated with solid tumor lymphatic leukemia P388 and dispersed into PRMI 1640 media harvested the leukemia P388 cells. Sixty P388 BALB/C mice were randomly grouped; 36 of them were used as nano-HAp treated groups and 24 mice as the control groups. The leukemia growth in the mice was examined morphologically, histopathologically and under a transmission electron microscope (TEM).


RESULTS
The nano-HAp was identified as a hydroxyapatite by an X-ray diffractometry (XRD) and a Fourier transform infrared spectroscopy (FTIR). The morphology and sizes were observed under a TEM. The tissue growth inhibition ratio (weight%) of solid lymphatic leukemia P388 bearing mice treated with nano-HAp at doses 35 mg/kg, 53 mg/kg and nano-HAp (53 mg/kg) combined with CY (35 mg/kg) in 3 consecutive days via intraperitineal injections were 14.95%, 32.67% and 60.45% respectively. Apoptosis of P388 cell cocultured with nano-HAp was confirmed by TEM.


CONCLUSIONS
The tissue growth restriction of solid tumor lymphatic leukemia P388 was greater after an injection of nano-HAp only or nano-HAp mixed with CY than that obtained after injection with physiological saline solution as a control (P < 0.01), and the tissue growth restriction of solid tumor after an injection of nano-HAp combined with CY was greater than that obtained after nano-HAp or CY injection only (P < 0.01).
Li
Ge
G
Department of Pediatrics, Sichuan Provincial People's Hospital, Chengdu 610072, China. ligejp2002@yahoo.com.cn
Huang
Jian-ming
JM
Aoki
Hideki
H
Li
Yan
Y
Zhang
Rong
R
Deng
Bi-fang
BF
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000737:chemistry; D045744:Cell Line, Tumor / Q000187:drug effects / Q000473:pathology; D017886:Durapatite / Q000494:pharmacology*; D005260:Female; D007941:Leukemia P388 / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D014961:X-Ray Diffraction / Q000379:methods; D014965:X-Rays
D001672:Biocompatible Materials; D017886:Durapatite; D002126:Calcium Hydroxide