Gordonia lacunae sp. nov., isolated from an estuary.
An actinomycete, strain BS2T, was isolated from a sand sample collected from an estuary in Plettenberg Bay, Western Cape Province, South Africa. Based on 16S rRNA gene sequence similarities and chemotaxonomy, strain BS2T was identified as a member of the genus Gordonia. It exhibited weak antibiosis against Mycobacterium aurum A+ and Mycobacterium smegmatis LR222. Phenotypic and phylogenetic results allowed for the differentiation of strain BS2T from other species within the genus Gordonia. DNA-DNA hybridization further differentiated strain BS2T from its nearest phylogenetic neighbour, Gordonia terrae NRRL B-16283T (57.5+/-4.4% DNA relatedness). Strain BS2T is therefore a novel species within the genus Gordonia, for which the name Gordonia lacunae is proposed, with the type strain being BS2T (=DSM 45085T=NRRL B-24551T).
le Roes
Marilize
M
Department of Molecular and Cell Biology, University of Cape Town, Private Bag 1, Rondebosch 7701, Cape Town, South Africa.
Goodwin
Candice M
CM
Meyers
Paul R
PR
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000898:Antibiosis; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000187:drug effects / Q000254:growth & development; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012822:Silicon Dioxide; D012988:Soil Microbiology; D013019:South Africa
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012822:Silicon Dioxide