title: Solid-state fermentation for enhanced production of laccase using indigenously isolated Ganoderma sp.
abstract: Laccase production by solid-state fermentation (SSF) using an indigenously isolated white rot basidiomycete Ganoderma sp. was studied. Among the various agricultural wastes tested, wheat bran was found to be the best substrate for laccase production. Solid-state fermentation parameters such as optimum substrate, initial moisture content, and inoculum size were optimized using the one-factor-at-a-time method. A maximum laccase yield of 2,400 U/g dry substrate (U/gds) was obtained using wheat bran as substrate with 70% initial moisture content at 25 degrees C and the seven agar plugs as the inoculum. Further enhancement in laccase production was achieved by supplementing the solid-state medium with additional carbon and nitrogen source such as starch and yeast extract. This medium was optimized by response surface methodology, and a fourfold increase in laccase activity (10,050 U/g dry substrate) was achieved. Thus, the indigenous isolate seems to be a potential laccase producer using SSF. The process also promises economic utilization and value addition of agro-residues.
authors.affiliation: Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai 400019, India.
authors.forename: Madhavi S
authors.initials: MS
authors.lastname: Revankar
authors.forename: Kiran M
authors.initials: KM
authors.lastname: Desai
authors.forename: S S
authors.initials: SS
authors.lastname: Lele
mesh_terms: D028023:Arecaceae; D001234:Aspergillus niger; D019149:Bioreactors; D005285:Fermentation; D032902:Ganoderma / Q000201:enzymology / Q000302:isolation & purification*; D007220:Industrial Waste; D042845:Laccase / Q000096:biosynthesis*; D020076:Pleurotus; D011124:Polyporaceae; D013379:Substrate Specificity
chemical_list: D007220:Industrial Waste; D042845:Laccase