In vitro differentiation of human mesenchymal stem cells on Ti6Al4V surfaces.
Long-term stability of arthroplasty prosthesis depends on the integration between the bone tissue and the implanted biomaterials, which requires the contribution of osteoblastic precursors and their continuous differentiation into the osteoblastic phenotype. Classically, these interactions are tested in vitro using mesenchymal stem cells (MSCs) isolated and ex vivo expanded from bone marrow aspirates. Human adipose tissue-derived stromal cells (AMSCs) may be a more convenient source of MSCs, according to their abundance and accessibility, but no data are available on their in vitro interactions with hard biomaterials. The aim of this work is to compare the osteogenic potential of human AMSCs and bone marrow-derived MSCs (BMMSCs) and to evaluate their response to Ti6Al4V alloy in terms of adhesion, proliferation and differentiation features, using the human osteosarcoma cell line SaOS-2 for comparison. The overall results showed that AMSCs have the same ability to produce bone matrix as BMMSCs and that Ti6Al4V surfaces exhibit an osteoinductive action on AMSCs, promoting their differentiation into functional osteoblasts and increasing bone formation. In conclusion, adipose tissue is a promising autologous source of osteoblastic cells with important clinical implications for bone tissue engineering.
Tognarini
Isabella
I
Department of Internal Medicine, Medical School, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy.
Sorace
Sabina
S
Zonefrati
Roberto
R
Galli
Gianna
G
Gozzini
Alessia
A
Carbonell Sala
Silvia
S
Thyrion
Giorgia D Zappoli
GD
Carossino
Anna Maria
AM
Tanini
Annalisa
A
Mavilia
Carmelo
C
Azzari
Chiara
C
Sbaiz
Fausto
F
Facchini
Alessandro
A
Capanna
Rodolfo
R
Brandi
Maria Luisa
ML
D000199:Actins / Q000378:metabolism; D000368:Aged; D000497:Alloys; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000166:cytology; D002448:Cell Adhesion; D002454:Cell Differentiation; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008875:Middle Aged; D010006:Osteoblasts / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D013499:Surface Properties; D014025:Titanium
D000199:Actins; D000497:Alloys; D015415:Biomarkers; D012333:RNA, Messenger; C031462:titanium alloy (TiAl6V4); D014025:Titanium