The value of combined use of survivin, cytokeratin 20 and mucin 7 mRNA for bladder cancer detection in voided urine.
OBJECTIVE
To evaluate the value of combined use of survivin, cytokeratin (CK) 20 and mucin (MUC) 7 mRNA in comparison with voided urine cytology in the detection of bladder cancer patients.


METHODS
One hundred and fifty three patients and 20 healthy volunteers were evaluated by RT-PCR for detecting survivin, CK-20 and MUC7 mRNA in voided urine before cystoscopy. The three markers and cytology were evaluated independently or in combinations.


RESULTS
The overall sensitivity and specificity were 90.4 and 94.7% for survivin, 82.6 and 97.4% for CK-20, 62.6 and 94.7% for MUC7 and 46.0 and 100% for voided urine cytology. Combined sensitivity of voided urine cytology with the three biomarkers together was higher than either combined sensitivity of voided urine cytology with one of the biomarkers or than that of the biomarker alone.


CONCLUSIONS
Combined use of the three markers can improve the sensitivity for detecting bladder cancer.
Pu
Xiao-Yong
XY
Department of Urology, Guangdong Provincial People's Hospital, Number 106, the 2nd Road of Zhongshan, Guangzhou, Guangdong 510080, China.
Wang
Zhi-Ping
ZP
Chen
Yi-Rong
YR
Wang
Xing-Huan
XH
Wu
Yi-Long
YL
Wang
Huai-Peng
HP
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D053554:Keratin-20 / Q000235:genetics*; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008875:Middle Aged; D009077:Mucins / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000652:urine*; D012471:Salivary Proteins and Peptides / Q000235:genetics*; D012680:Sensitivity and Specificity; D000077022:Survivin; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000652:urine; D014556:Urine / Q000166:cytology*
C107489:BIRC5 protein, human; D014408:Biomarkers, Tumor; D051034:Inhibitor of Apoptosis Proteins; D053554:Keratin-20; C083238:MUC7 protein, human; D008869:Microtubule-Associated Proteins; D009077:Mucins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012471:Salivary Proteins and Peptides; D000077022:Survivin