title: [Synergistic effects of acitretin and narrow-band ultraviolet-B in inducing retinoic acid receptor gamma mRNA expression in normal human keratinocytes].
abstract: OBJECTIVE
  To investigate the changes in cell proliferation and retinoic acid receptor gamma (RARgamma) mRNA expression in normal human keratinocytes after acitretin treatment and/or narrow-band ultraviolet-B irradiation.
  
  
  METHODS
  Normal human keratinocytes were exposed to irradiation with 100 mJ/cm square NB-UVB and/or subsequent 12-hour incubation with 1x10(-6) mol/L acitretin, and the expression of RARgamma mRNA in the cells was examined using RT-PCR and real-time quantitative RT-PCR.
  
  
  RESULTS
  A 0.9- and a 2.3-fold increase in RARgamma mRNA expression was induced in the cells by exposure to 100 mJ/cm square NB-UVB and 10(-6) mol/L acitretin, respectively, and the expression was synergistically enhanced by 2.8-fold after their combined treatment.
  
  
  CONCLUSIONS
  Upregulated expression of RARgamma mRNA can be associated with keratinocyte growth inhibition after treatment with acitretin and NB-UVB irradiation.
authors.affiliation: Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. luosuju2005@163.com
authors.forename: Su-ju
authors.initials: SJ
authors.lastname: Luo
authors.forename: Zhen-hui
authors.initials: ZH
authors.lastname: Peng
authors.forename: Yan
authors.initials: Y
authors.lastname: Zheng
authors.forename: Hao-xiang
authors.initials: HX
authors.lastname: Xu
authors.forename: Shao-na
authors.initials: SN
authors.lastname: Zhou
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Li
authors.forename: Guo-rong
authors.initials: GR
authors.lastname: Wang
mesh_terms: D017255:Acitretin / Q000494:pharmacology*; D002478:Cells, Cultured; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000528:radiation effects*; D012333:RNA, Messenger / Q000378:metabolism; D018168:Receptors, Retinoic Acid / Q000378:metabolism*; D014466:Ultraviolet Rays; D000097768:Retinoic Acid Receptor gamma
chemical_list: D017255:Acitretin; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D000097768:Retinoic Acid Receptor gamma