Crystallization and preliminary X-ray diffraction analysis of maize aldose reductase.
Maize aldose reductase (AR) is a member of the aldo-keto reductase superfamily. In contrast to human AR, maize AR seems to prefer the conversion of sorbitol into glucose. The apoenzyme was crystallized in space group P2(1)2(1)2(1), with unit-cell parameters a = 47.2, b = 54.5, c = 100.6 A and one molecule in the asymmetric unit. Synchrotron X-ray diffraction data were collected and a final resolution limit of 2.0 A was obtained after data reduction. Phasing was carried out by an automated molecular-replacement procedure and structural refinement is currently in progress. The refined structure is expected to shed light on the functional/enzymatic mechanism and the unusual activities of maize AR.
Kiyota
Eduardo
E
Laboratório de Biologia Estrutural, Instituto de Química, Universidade Estadual de Campinas, CP 6154, 13083-970 Campinas-SP, Brazil.
de Sousa
Sylvia Morais
SM
Dos Santos
Marcelo Leite
ML
da Costa Lima
Aline
A
Menossi
Marcelo
M
Yunes
José Andrés
JA
Aparicio
Ricardo
R
D000449:Aldehyde Reductase / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D003313:Zea mays / Q000201:enzymology*
D000449:Aldehyde Reductase