title: Feasibility of direct oxygenation of primary-cultured rat hepatocytes using polyethylene glycol-decorated liposome-encapsulated hemoglobin (LEH).
abstract: We tested the short-term efficacy of liposome-encapsulated hemoglobin (LEH) in cultured rat hepatocytes. Supplementation with LEH (20% of the hemoglobin concentration of blood) did not lower albumin production in static culture, and completely reversed the cell death and deterioration in albumin production caused by an oxygen shortage in 2D flat-plate perfusion bioreactors.
authors.affiliation: Institute of Industrial Science (IIS), University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
authors.forename: Hirosuke
authors.initials: H
authors.lastname: Naruto
authors.forename: Hongyun
authors.initials: H
authors.lastname: Huang
authors.forename: Masaki
authors.initials: M
authors.lastname: Nishikawa
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Kojima
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mizuno
authors.forename: Katsuji
authors.initials: K
authors.lastname: Ohta
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Sakai
mesh_terms: D000818:Animals; D002214:Capsules; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D005240:Feasibility Studies; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry*; D008297:Male; D010100:Oxygen / Q000008:administration & dosage* / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002214:Capsules; D020099:Coated Materials, Biocompatible; D006454:Hemoglobins; D008081:Liposomes; D011092:Polyethylene Glycols; D010100:Oxygen