[Effects of laminar shear stress on the expression of IL-8 receptor CXCR1 in endothelial cells].
This study was conducted to elucidate the effects of different fluid shear stress on the expression of IL-8 receptors CXCR1 in endothelial cells. The HUVEC cell lines, EA. Hy926 cells, were cultured in vitro, exposed to 5.56, 10.02, and 15.27 dyn/cm2 laminar shear stress, respectively, and then the shear stress grous were each investigated at the time-points of 0h, 1h, 2h, 4h and 8h. Western blotting was used for detecting the expression of IL-8 receptor CXCR1 at the time-points. The results showed that, under 5.56 dyn/cm2 shear stress, the expression of CXCR1 increased with time significantly (P < 0.01). The maximum expression of CXCR1 appeared at 4 h and was 2.2 times that of the control. When exposed to 10.02 dyn/cm2, the expression of CXCR1 increased gradually with time and finally remained at a constant higher level. When exposed to 15.27 dyn/cm2, CXCR1 expression decreased significantly with time (P < 0.01). The minimum CXCR1 expression appeared at 8 h and was 62. 59 percent of that of the control. These results indicate that the expression of CXCR1 in endothelial cell is regulated by laminar shear stress.
Xue
Longjuan
L
Institute of Biomedical Engineering, West China Medical Center, Sichuan University, Chengdu 610041, China.
Yu
Chang
C
Liu
Xiaoheng
X
Lai
Yi
Y
Zeng
Ye
Y
Zhang
Yi
Y
D002460:Cell Line; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D023062:Receptors, Interleukin-8A / Q000235:genetics / Q000378:metabolism*; D033081:Shear Strength; D013314:Stress, Mechanical; D014471:Umbilical Veins / Q000166:cytology
D012333:RNA, Messenger; D023062:Receptors, Interleukin-8A