[Studies on the extraction of DNA using carboxyl-coated magnetic particles and quantification using fluorescent dye].
Based on the study of extraction of DNA using carboxyl-coated Fe3O4, here we provide a rapid, high efficient, safe and economical method. Using fluorescent dye-Sybr Green I, the quantification of double-strand DNA exhibits the advantages of high sensitivity,good repeatability and convenience.
Ma
Yongjie
Y
National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Shanghai Jiaotong University, Shanghai 200030, China.
Gu
Hongchen
H
Zheng
Weiming
W
D052160:Benzothiazoles; D002245:Carbon Dioxide / Q000737:chemistry*; D004247:DNA / Q000097:blood / Q000302:isolation & purification*; D003959:Diamines; D005290:Ferric Compounds / Q000737:chemistry; D005456:Fluorescent Dyes; D006801:Humans; D008280:Magnetics; D053758:Nanoparticles; D009930:Organic Chemicals; D010316:Particle Size; D011804:Quinolines; D013194:Staining and Labeling
D052160:Benzothiazoles; D003959:Diamines; D005290:Ferric Compounds; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; D002245:Carbon Dioxide; C041069:carboxyl radical; C098022:SYBR Green I; C000499:ferric oxide; D004247:DNA