The effect of temperature increase on the expression of manganese superoxide dismutase in tissues of common carp Cyprinus carpio.
The increase of environmental temperature at physiological range can cause oxidative stress in exotherms, which in most cases leads to activation of the antioxidant enzyme, manganese-1 superoxide dismutase (Mn-SOD). This work is aimed to evaluate the changes in Mn-SOD enzyme activity and mRNA levels in the common carp, Cyprinus carpio, during transition from lower (15 degrees C) to higher (25 and 30 degrees C) temperatures. In liver, 25 degrees C exposure elicited little effect, but at 30 degrees C there was a significant increase in both Mn-SOD enzyme activity and mRNA levels. In brain enzyme activity was maximal at 25 degrees C and surprisingly, this increased activity was accompanied by a decrease in mRNA levels. This work suggests that the activity of Mn-SOD in carp is regulated by environmental conditions through transcriptional, translational and post-translational mechanisms, the particular mechanism used being dependent upon the tissue type.
Lushchak
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VI
Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine. lushchak@pu.if.ua
Murray
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P
Cossins
A R
AR
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002347:Carps / Q000254:growth & development / Q000378:metabolism*; D002374:Catalase / Q000096:biosynthesis; D004790:Enzyme Induction; D008099:Liver / Q000201:enzymology*; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000096:biosynthesis; D013482:Superoxide Dismutase / Q000096:biosynthesis*; D013696:Temperature
D012333:RNA, Messenger; D002374:Catalase; D013482:Superoxide Dismutase