title: Self-assembly of proximity probes for flexible and modular proximity ligation assays.
abstract: Proximity ligation assay (PLA) is a recently developed strategy for protein analysis in which antibody-based detection of a target protein via a DNA ligation reaction of oligonucleotides linked to the antibodies results in the formation of an amplifiable DNA strand suitable for analysis. Here we describe a faster and more cost-effective strategy to construct the antibody-based proximity ligation probes used in PLA that is based on the noncovalent interaction of biotinylated oligonucleotides with streptavidin followed by the interaction of this complex with biotinylated antibodies.
authors.affiliation: Department of Genetics and Pathology, Uppsala University, Sweden.
authors.forename: Spyros
authors.initials: S
authors.lastname: Darmanis
authors.forename: Anna
authors.initials: A
authors.lastname: Kähler
authors.forename: Lena
authors.initials: L
authors.lastname: Spångberg
authors.forename: Masood
authors.initials: M
authors.lastname: Kamali-Moghaddam
authors.forename: Ulf
authors.initials: U
authors.lastname: Landegren
authors.forename: Edith
authors.initials: E
authors.lastname: Schallmeiner
mesh_terms: D000906:Antibodies / Q000378:metabolism*; D011088:DNA Ligases / Q000378:metabolism*; D015342:DNA Probes / Q000235:genetics*; D007118:Immunoassay / Q000379:methods*; D015336:Molecular Probe Techniques; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D015342:DNA Probes; D011506:Proteins; D011088:DNA Ligases