Supramolecular complex formation by beta-cyclodextrin and ferrocenylnaphthalene diimide-intercalated double stranded DNA and improved electrochemical gene detection.
Ferrocenylnaphthalene diimide 1 can bind to double stranded DNA (dsDNA) by the threading intercalation mode and the resulting complex was stabilized further by beta- cyclodextrin (CD) by forming a supramolecular complex. These complex formation processes were studied by spectroscopic, viscometric, and electrochemical means in the absence or presence of beta-CD. Quantitative analysis by quartz crystal microbalance (QCM) and electrochemical experiments strongly suggested a 2:1 binding stoichiometry for beta-CD to 1 threading-intercalated to the dsDNA-immobilized electrode. Owing to this supramolecular complex formation, electrochemical DNA detection based on 1 was improved considerably.
Sato
Shinobu
S
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka 812-8581, Japan.
Nojima
Takahiko
T
Waki
Michinori
M
Takenaka
Shigeori
S
D004247:DNA / Q000032:analysis* / Q000378:metabolism; D015342:DNA Probes; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005296:Ferrous Compounds; D007094:Imides; D007364:Intercalating Agents; D046911:Macromolecular Substances; D047392:beta-Cyclodextrins
D015342:DNA Probes; D005296:Ferrous Compounds; D007094:Imides; D007364:Intercalating Agents; D046911:Macromolecular Substances; D047392:beta-Cyclodextrins; C406363:ferrocenylnaphthalene diimide; D004247:DNA; C031215:betadex