[Expression of B7-H1 on peripheral blood mononuclear cells from chronic hepatitis B patients].
OBJECTIVE
To investigate the expression of B7-H1 on peripheral blood mononuclear cells from patients with chronic HBV infection.


METHODS
Immunofluorescent staining and flow cytometry assay were used to measure the expression of B7-H1 on peripheral blood CD3high T cells, CD19high B cells and CD14high monocytes from chronic HBV infected patients.


RESULTS
No significant difference was observed in B7-H1 expression on T cells and B cells between chronic HBV infected patients (CHB) and health controls (HC). B7-H1-expressing CD14high cells were significantly increased in chronic HBV-infected patients (19.17-/+11.64)% as compared with healthy controls [(7.30-/+5.49)%, P<0.01]. A significant positive correlation was found between B7-H1 expression on CD14high monocytes and serum ALT levels.


CONCLUSIONS
There is no significant difference in B7-H1 expression on T cells and B cells between CHB patients and healthy subjects. B7-H1, which is up-regulated on monocytes from chronic HBV-infected patients, in positively correlated to serum ALT levels, and may play a role in the persistence of HBV infection.
Xie
Zhun-yi
ZY
Institute of Immunology of PLA, Third Military Medical University, Chongqing 400038, China. 3132xzy@mail.tmmu.com.cn
Chen
Yong-wen
YW
Fu
Xiao-lan
XL
Yang
Cheng-ying
CY
Wu
Yu-zhang
YZ
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology; D060890:B7-H1 Antigen; D016022:Case-Control Studies; D005260:Female; D019694:Hepatitis B, Chronic / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D055815:Young Adult
D015703:Antigens, CD; D060890:B7-H1 Antigen; C423236:CD274 protein, human