Gene manipulation of adenovirus vector by artificial restriction DNA cutter.
Adenovirus vector is a useful tool for research in gene expression and regulation as well as gene therapy. However, the size of this vector is too large to construct it by a simple method. In this study, we successfully cleaved specifically at one target site of this large vector by artificial restriction DNA cutter (ARCUT). This result suggests that ARCUT will be the powerful tool for construction of large sized virus vectors.
Shigi
Narumi
N
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Otani
Kosuke
K
Komiyama
Makoto
M
D000256:Adenoviridae / Q000235:genetics*; D002563:Cerium / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry; D005822:Genetic Vectors / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*
D020135:Peptide Nucleic Acids; D002563:Cerium; D004492:Edetic Acid