title: Effect of a Bowman-Birk proteinase inhibitor from Phaseolus coccineus on Hypothenemus hampei gut proteinases in vitro.
abstract: The coffee berry borer, Hypothenemus hampei (Ferrari), is an important devastating coffee pest worldwide. Both trypsin and chymotrypsin enzyme activities from H. hampei larval midgut can be inactivated by proteinaceous enzyme-inhibitors. A serine proteinase inhibitor belonging to the Bowman-Birk class was purified from a wild accession of Phaseolus coccineus L. seeds. The inhibitor (PcBBI1) is a cysteine-rich protein that is heat-stable at alkaline pH. MALDI-TOF/MS analysis showed that PcBBI1 occurs in seeds as a monomer (8689 Da) or dimer (17,378 Da). Using in vitro inhibition assays, it was found that PcBBI1 has a high inhibitory activity against H. hampei trypsin-like enzymes, bovine pancreatic chymotrypsin, and trypsin. According to this, PcBBI1 could be a promising tool to make genetically modified coffee with resistance to coffee berry borer.
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, PqEB, W5 Norte Final, Brasília - DF, 70770-900, Brazil.
authors.forename: Railene
authors.initials: R
authors.lastname: de Azevedo Pereira
authors.forename: Arnubio
authors.initials: A
authors.lastname: Valencia-Jiménez
authors.forename: Cláudio Picanço
authors.initials: CP
authors.lastname: Magalhães
authors.forename: Maura Vianna
authors.initials: MV
authors.lastname: Prates
authors.forename: Jorge Alex Taquita
authors.initials: JA
authors.lastname: Melo
authors.forename: Liziane Maria
authors.initials: LM
authors.lastname: de Lima
authors.forename: Maurício Pereira
authors.initials: MP
authors.lastname: de Sales
authors.forename: Erich Yukio
authors.initials: EY
authors.lastname: Tempel Nakasu
authors.forename: Maria Cristina Mattar
authors.initials: MC
authors.lastname: da Silva
authors.forename: Maria Fátima
authors.initials: MF
authors.lastname: Grossi-de-Sá
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001517:Coleoptera / Q000201:enzymology*; D007814:Larva / Q000201:enzymology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism*; D027805:Phaseolus / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D015842:Serine Proteinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D015842:Serine Proteinase Inhibitors; D010447:Peptide Hydrolases