pH-controlled construction of chitosan/alginate multilayer film: characterization and application for antibody immobilization.
In this work, a chitosan/alginate multilayer film was constructed via layer-by-layer self-assembly and studied by in situ surface plasmon resonance monitoring and contact angle measurements. The results demonstrate that the surface composition of the self-assembled multilayer film can be simply tailored through pH control during the assembly process. The biological property of the assembled film was further characterized via antigen-antibody interactions, showing that the loading capacity of the antibody on the multilayered film and the binding activity of the antigen to the immobilized antibody could be also well-tuned by pH control. This work can provide more scientific insight in the interaction between protein and polymer matrix and render a novel simple approach to build high-performance biointerfaces through pH control for potential applications of highly sensitive immunosensors.
Yuan
Weiyong
W
School of Chemical & Biomedical Engineering, Center for Advanced Bionanosystems, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457.
Dong
Hua
H
Li
Chang Ming
CM
Cui
Xiaoqiang
X
Yu
Ling
L
Lu
Zhisong
Z
Zhou
Qin
Q
D000464:Alginates / Q000737:chemistry*; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D000936:Antigen-Antibody Complex; D048271:Chitosan / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D018625:Microscopy, Atomic Force; D020349:Surface Plasmon Resonance
D000464:Alginates; D000906:Antibodies; D000936:Antigen-Antibody Complex; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D048271:Chitosan