Searching study for the suitable ferrocenylnaphthalene diimide derivative in the electrochemical telomerase assay.
Interaction of ferrocenylnaphthalene diimide 1 with the tetraplex oligonucleotide, AGGG(TTAGGG)3, which is a part of human telomere sequence, was studied in 0.1 M AcONa-AcOH (pH 5.5) containing 0.1 M NaCl coupled with 22-meric single and double stranded oligonucleotides using spectrophotometric titration experiment to search for the more suitable tetraplex DNA-binding ligand to achieve the electrochemical telomerase assay. CD spectra and polyacrylamide gel electrophoresis revealed that this tetraplex oligonucleotide kept to the single conformational structure of basket type G-quadruplex under these conditions. Scatchard analysis showed that 1 can bind to the tetraplex oligonucleotide with the binding constant of 10(5) M(-1) order, which was the highest value among the other oligonucleotides. Binding number of 1 for these oligonucleotides were ca. 2, 11, and 3 for tetraplex, double stranded, and single stranded oligonucleotides, respectively. These values were reasonable when considering with the binding mode of 1 as a threading intercalator.
Sato
Shinobu
S
Department of Bioscience and Bioinformatics, Faculty of Computer Science and Systems Engineering, 680-4 Kawazu, Iizuka-shi, Fukuoka, 820-8502, Japan.
Ohtsuka
Keiichi
K
Takenaka
Shigeori
S
D001483:Base Sequence; D004563:Electrochemistry; D005296:Ferrous Compounds / Q000737:chemistry*; D054856:G-Quadruplexes; D006801:Humans; D007094:Imides / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D019098:Telomerase / Q000032:analysis*; D016615:Telomere / Q000737:chemistry*
D005296:Ferrous Compounds; D007094:Imides; D009841:Oligonucleotides; C406363:ferrocenylnaphthalene diimide; D019098:Telomerase