title: Bone marrow microvessel density in chronic myeloproliferative disorders: a study of 115 patients with clinicopathological and molecular correlations.
abstract: Philadelphia-negative chronic myeloproliferative disorders (CMD) include polycythemia vera (PV), essential thrombocythemia (ET) and primary myelofibrosis (PMF). Angiogenesis is critical in the pathogenesis of PMF. We studied angiogenesis in 115 patients with CMD (23 PV, 24 ET, 46 PMF, 12 post-PV and 10 post-ET myelofibrosis) by assessment of microvessel density (MVD) in bone marrow (BM). Kruskall-Wallis analysis of variance showed that patients with PMF had significantly higher values of MVD than those with PV (P < 0.001), ET (P < 0.001) and controls (P < 0.001). Mann-Whitney U-test demonstrated that patients with PMF at the prefibrotic stage had significantly higher MVD values than those with ET (P = 0.02). Patients with post-PV myelofibrosis showed significantly higher MVD values than those with PV (P < 0.001), as did patients with post-ET myelofibrosis compared with ET (P < 0.001). In patients with CMD, the multivariate generalized linear regression model showed that the JAK2 (V617F) mutational burden (P = 0.01), serum lactate dehydrogenase level (P = 0.003), and anaemia (P < 0.001) independently correlated with MVD. In summary, this study indicates that assessment of BM angiogenesis, as measured by MVD, may be a useful additional tool in the histopathological definition of CMD.
authors.affiliation: Department of Surgical Pathology, University of Pavia Medical School, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Boveri
authors.forename: Francesco
authors.initials: F
authors.lastname: Passamonti
authors.forename: Elisa
authors.initials: E
authors.lastname: Rumi
authors.forename: Daniela
authors.initials: D
authors.lastname: Pietra
authors.forename: Chiara
authors.initials: C
authors.lastname: Elena
authors.forename: Luca
authors.initials: L
authors.lastname: Arcaini
authors.forename: Cristiana
authors.initials: C
authors.lastname: Pascutto
authors.forename: Alessandro
authors.initials: A
authors.lastname: Castello
authors.forename: Mario
authors.initials: M
authors.lastname: Cazzola
authors.forename: Umberto
authors.initials: U
authors.lastname: Magrini
authors.forename: Mario
authors.initials: M
authors.lastname: Lazzarino
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000740:Anemia / Q000209:etiology; D001853:Bone Marrow / Q000098:blood supply*; D002908:Chronic Disease; D005260:Female; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009196:Myeloproliferative Disorders / Q000150:complications / Q000235:genetics / Q000473:pathology*; D009389:Neovascularization, Pathologic / Q000473:pathology*; D011087:Polycythemia Vera / Q000235:genetics / Q000473:pathology; D055728:Primary Myelofibrosis / Q000235:genetics / Q000473:pathology; D013920:Thrombocythemia, Essential / Q000235:genetics / Q000473:pathology
chemical_list: D007770:L-Lactate Dehydrogenase; C507924:JAK2 protein, human; D053614:Janus Kinase 2