title: Microarray-based approach for genome-wide survey of nucleotide polymorphisms.
abstract: DNA microarrays can be used to detect polymorphic loci in addition to identifying genes or regions that are absent within a genome. A survey such as this offers greater insight into the level of diversification within a species or population, which is useful in organisms that have near-identical genomic content but differ in phenotype. The identification of such variable loci can then lead to the characterization of genes linked to unique biological attributes. Here, we describe a competitive hybridization assay using DNA microarrays as a comparative genomics tool to identify nucleotide polymorphisms among closely related strains of Chlamydia trachomatis.
authors.forename: Brian W
authors.initials: BW
authors.lastname: Brunelle
authors.forename: Tracy L
authors.initials: TL
authors.lastname: Nicholson
mesh_terms: D002692:Chlamydia trachomatis / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D006367:HeLa Cells; D006801:Humans; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide
chemical_list: D004269:DNA, Bacterial