[Effects of cinnamyl aldehyde on cell cycle and relafeol proteins expression in NIH3T3 cells].
OBJECTIVE
To observe the effects of Cinnamyl aldehyde (CA) on NIH3T3 cell cycle and explore the possible mechanism further.


METHODS
Flowcytometry was used for observing cell cycle distribution. Expressions of proliferation cell nuclear antigen (PCNA) and Cyclin D1 protein in NIH3T3 cells were assessed by immunocytochemistry.


RESULTS
After culture with CA for 24 hours, the percentage of populations of S phase was enhanced by 3% (P < 0.05) and cell proliferation index (PrI, S + G2/M) was increased by 3.5% (P < 0.01) , but G2/M phase had no obvious changes. The expressions of Cyclin D1 and PCNA proteins were improved markly by CA compared with controlgroup (P < 0.01).


CONCLUSIONS
CA could promote more cells in G0/G1 phase into S phase, which may be related to the regulation of the expressions of PCNA and Cyclin D1.
Zhao
Jing-xia
JX
Beijing Institute of Chinese Medicine, Beijing 100010, China.
Li
Ping
P
Sheng
Xun
X
Liu
Xin
X
Liang
Dai-ying
DY
D000171:Acrolein / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D027424:Cinnamomum / Q000737:chemistry; D019938:Cyclin D1 / Q000378:metabolism*; D005434:Flow Cytometry; D007150:Immunohistochemistry; D051379:Mice; D041681:NIH 3T3 Cells; D010946:Plants, Medicinal / Q000737:chemistry; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D016196:S Phase / Q000187:drug effects
D018809:Proliferating Cell Nuclear Antigen; D019938:Cyclin D1; D000171:Acrolein; C012843:cinnamaldehyde