Enhancement of gene silencing potency and nuclease stability by chemically modified duplex RNA.
In this study, we describe a development of chemically modified dsRNAs with improved biological properties. These dsRNAs possess an enhanced RNAi activity and a dramatically increased stability in cell cultured medium (containing 10% serum) in comparison with widely used 21nt siRNA. The chemically modified dsRNAs manifested a high longterm gene suppression after one week post-transfection, whereas 21nt siRNA showed a high RNAi activity only after 48 h cell transfection.
Kubo
Takanori
T
On-site Sensing and Diagnosis Research Laboratory, Advanced Industrial Sicence and Technology, 807-1, Shukumachi, Tosu 841-0052, Japan. kubo-jori@aist.go.jp
Zhelev
Zhivko
Z
Bakalova
Rumiana
R
Ohba
Hideki
H
D000588:Amines / Q000737:chemistry; D017930:Genes, Reporter; D034622:RNA Interference; D020871:RNA Stability; D012330:RNA, Double-Stranded / Q000737:chemistry* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000737:chemistry / Q000378:metabolism; D043244:Ribonuclease III / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism
D000588:Amines; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D012260:Ribonucleases; D043244:Ribonuclease III