Sequence specific interstrand photocrosslinking for effective SNP typing.
We describe a simple and inexpensive SNP typing method by using sequence specific interstrand photocrosslinking via p-carbamoylvinyl phenol nucleosides. Interstrand photocrosslinking showed a high degree of single nucleotide specificity as high as 10(3)-fold and more, and can be used in the diagnostic detection of DNA sequences.
Ami
Takehiro
T
School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa 923-1292, Japan.
Ito
Katsuya
K
Yoshimura
Yoshinaga
Y
Fujimoto
Kenzo
K
D001483:Base Sequence; D003432:Cross-Linking Reagents / Q000737:chemistry* / Q000528:radiation effects; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000737:chemistry / Q000528:radiation effects; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010636:Phenols / Q000737:chemistry / Q000528:radiation effects; D010777:Photochemistry; D020641:Polymorphism, Single Nucleotide / Q000528:radiation effects; D017422:Sequence Analysis, DNA / Q000379:methods*; D013379:Substrate Specificity; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D010636:Phenols; D004247:DNA