Feasibility of direct oxygenation of primary-cultured rat hepatocytes using polyethylene glycol-decorated liposome-encapsulated hemoglobin (LEH).
We tested the short-term efficacy of liposome-encapsulated hemoglobin (LEH) in cultured rat hepatocytes. Supplementation with LEH (20% of the hemoglobin concentration of blood) did not lower albumin production in static culture, and completely reversed the cell death and deterioration in albumin production caused by an oxygen shortage in 2D flat-plate perfusion bioreactors.
Naruto
Hirosuke
H
Institute of Industrial Science (IIS), University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
Huang
Hongyun
H
Nishikawa
Masaki
M
Kojima
Nobuhiko
N
Mizuno
Atsushi
A
Ohta
Katsuji
K
Sakai
Yasuyuki
Y
D000818:Animals; D002214:Capsules; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D005240:Feasibility Studies; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry*; D008297:Male; D010100:Oxygen / Q000008:administration & dosage* / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D002214:Capsules; D020099:Coated Materials, Biocompatible; D006454:Hemoglobins; D008081:Liposomes; D011092:Polyethylene Glycols; D010100:Oxygen