title: A comparative study of adaptive registration methods for two dimensional gel electrophoresis images.
abstract: Two-dimensional gel electrophoresis (2DGE) images play a major role in techniques for protein separation. The registration of 2DGE images is considered as one of key elements in protein identification. This paper proposes a single adaptive registration scheme for 2DGE images. Within the scheme, three registration methods based on the combined use of global linear affine and local nonlinear thin-plate spline (TPS), Demons, B-spline algorithms are explored and compared. The results show that the method using multi-resolution B-spline is preferred.
authors.affiliation: CREATIS-LRMN, CNRS, UMR 5220, Villeurbanne, F-69621, France. huamei.xin@creatis.insa-lyon.fr
authors.forename: Huamei
authors.initials: H
authors.lastname: Xin
authors.forename: Yuemin
authors.initials: Y
authors.lastname: Zhu
authors.forename: Longfei
authors.initials: L
authors.lastname: Wu
mesh_terms: D000465:Algorithms; D001185:Artificial Intelligence; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique