Effect of poly(L-lysine)-g-dextran copolymers on DNA hybridization.
We have focused on design of biomaterials that are capable of engineering nucleic acids folding. In this study, we explored the effect of poly(L-lysine)-g-dextran, PLL-g-Dex, copolymer on hybridization kinetics of DNA. The hybridization kinetics was studied by using a fluorescence resonance energy transfer (FRET) assay in the absence and presence of the copolymer. The copolymer at nano molar concentration significantly accelerated the hybridization rate over 200-fold under physiologically relevant ionic conditions.
Wu
Longliang
L
Institute for Materials Chemistry and Engineering, Kyushu University, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan.
Shimada
Naohiko
N
Kano
Arihiro
A
Maruyama
Atsushi
A
D004247:DNA / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D009693:Nucleic Acid Hybridization; D011107:Polylysine / Q000031:analogs & derivatives* / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry
D003911:Dextrans; C113090:poly(lysine)-graft-dextran; D011107:Polylysine; D012965:Sodium Chloride; D004247:DNA