title: Development of HPTLC-UV absorption densitometry method for the analysis of alprazolam and sertraline in combination and its application in the evaluation of marketed preparations.
abstract: A new simple, sensitive, and reproducible high-performance thin-layer chromatography method for the estimation of alprazolam and sertraline in combination is developed using silica gel plates with fluorescent indicators. The system is equipped with an automated sample applicator, and the detection was performed at 254 nm by using UV absorption densitometry. The mobile phase consists of carbon tetrachloride, methanol, acetone, and ammonia in the ratio 12:3:5:0.1. The retention factor values for alprazolam and sertraline are found to be 0.52 and 0.70, respectively. The limit of detection of alprazolam and sertraline in the mixture of given proportion is observed to be 0.05 microg/mL and 2.5 microg/mL and the limit of quantitation is 0.2 microg/mL and 10 microg/mL, respectively. The method has shown good linearity in the range of 0.2 microg/mL to 0.65 pg/mL for alprazolam (R2 > 0.9953) and 10 pg/mL to 32.5 microg/mL for sertraline (R2 > 0.9942). The intra- and inter-assay (n=5) variations in the linear range are less than 4% for alprazolam and 6% for sertraline. Three pharmaceutical products containing this combination are analyzed to test the applicability of the new method. The percentage of alprazolam and sertraline in the tablets studied range from 97.7% to 102.82% and 96.5% to 99.9%, respectively.
authors.affiliation: Department of Pharmaceutical Analysis, Goa College of Pharmacy, Panaji, Goa, India. vrk10@hotmail.com
authors.forename: K
authors.initials: K
authors.lastname: Venkateswarlu
authors.forename: R K
authors.initials: RK
authors.lastname: Venisetty
authors.forename: N R
authors.initials: NR
authors.lastname: Yellu
authors.forename: S
authors.initials: S
authors.lastname: Keshetty
authors.forename: M G
authors.initials: MG
authors.lastname: Pai
mesh_terms: D000525:Alprazolam / Q000032:analysis*; D014151:Anti-Anxiety Agents / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods*; D012015:Reference Standards; D017367:Selective Serotonin Reuptake Inhibitors / Q000032:analysis*; D020280:Sertraline / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D000525:Alprazolam; D014151:Anti-Anxiety Agents; D017367:Selective Serotonin Reuptake Inhibitors; D020280:Sertraline