title: T-loop formation by human telomeric G-quadruplex.
abstract: T-loops were a proposed telomere structure and have been speculated to protect telomeres and regulate telomerase. In the current study, we examined the structural and thermodynamic properties of the dimeric G-quadruplex of human telomeric DNA using chemical modifications and CD spectroscopy. We demonstrated that the dimeric G-quadruplex topology induced the T-loop formation in K(+) solution.
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sato
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Shinohara
authors.forename: Makoto
authors.initials: M
authors.lastname: Komiyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry; D019281:Dimerization; D054856:G-Quadruplexes; D006801:Humans; D008958:Models, Molecular; D016615:Telomere / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D004247:DNA