Effect of a single AGE modification on the structure and chaperone activity of human alphaB-crystallin.
During aging, human lens proteins undergo several post-translational modifications, one of which is glycation. This process leads to the formation of advanced glycation end products (AGEs) which accumulate with time possibly leading to the formation of cataract. alphaB-Crystallin, a predominant protein in the lens, is a member of the small heat shock proteins (sHSPs) which are a ubiquitous class of molecular chaperones that interact with partially denatured proteins to prevent aggregation. This chaperone function is considered to be vital for the maintenance of lens transparency and in the prevention of cataract. In the present study, we introduced an analog of the advanced glycation end product, OP-lysine, at the 90th position of a mutated human alphaB-crystallin (K90C) by covalent modification of the cysteine residue with N-(2-bromoethyl)-3-oxidopyridinium hydrobromide. The AGE-modified K90C-alphaB-crystallin is termed as K90C-OP. We compared the structural and functional properties of K90C-OP with the original K90C mutant, with K90C chemically modified back to a lysine analog (K90C-AE), and with wild-type human alphaB-crystallin. Modified K90C-OP showed decreased intrinsic tryptophan fluorescence and bis-ANS binding without significant alterations in either the secondary, tertiary, or quaternary structure. K90C-OP, however, exhibited a reduced efficiency in the chaperoning ability with alcohol dehydrogenase, insulin, and citrate synthase as substrates compared to the other alpha-crystallin proteins. Therefore, introduction of a single AGE near the chaperone site of human alphaB-crystallin can alter the chaperoning ability of the protein with only minor changes in the local environment of the protein.
Bhattacharyya
Jaya
J
Mason Eye Institute, University of Missouri, 404 Portland Street, Columbia, Missouri 65201, USA.
Shipova
Ekaterina V
EV
Santhoshkumar
Puttur
P
Sharma
K Krishna
KK
Ortwerth
Beryl J
BJ
D002942:Circular Dichroism; D003545:Cysteine / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D005453:Fluorescence; D017127:Glycation End Products, Advanced / Q000737:chemistry*; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives / Q000737:chemistry; D018832:Molecular Chaperones / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D011485:Protein Binding; D011726:Pyridinium Compounds / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D053719:Tandem Mass Spectrometry; D014364:Tryptophan / Q000737:chemistry; D038203:alpha-Crystallin B Chain / Q000737:chemistry* / Q000378:metabolism
C484321:2-amino-6-(3-oxidopyridinium-1-yl)hexanoic acid; D017127:Glycation End Products, Advanced; D018832:Molecular Chaperones; D011726:Pyridinium Compounds; D038203:alpha-Crystallin B Chain; D014364:Tryptophan; D008239:Lysine; D003545:Cysteine