Characterization of the mid-foregut transcriptome identifies genes regulated during lung bud induction.
To identify genes expressed during initiation of lung organogenesis, we generated transcriptional profiles of the prospective lung region of the mouse foregut (mid-foregut) microdissected from embryos at three developmental stages between embryonic day 8.5 (E8.5) and E9.5. This period spans from lung specification of foregut cells to the emergence of the primary lung buds. We identified a number of known and novel genes that are temporally regulated as the lung bud forms. Genes that regulate transcription, including DNA binding factors, co-factors, and chromatin remodeling genes, are the main functional groups that change during lung bud formation. Members of key developmental transcription and growth factor families, not previously described to participate in lung organogenesis, are expressed in the mid-foregut during lung bud induction. These studies also show early expression in the mid-foregut of genes that participate in later stages of lung development. This characterization of the mid-foregut transcriptome provides new insights into molecular events leading to lung organogenesis.
Millien
Guetchyn
G
Department of Medicine, Boston University School of Medicine, Evans Biomedical Research Center, Boston, MA 02118, USA.
Beane
Jennifer
J
Lenburg
Marc
M
Tsao
Po-Nien
PN
Lu
Jining
J
Spira
Avrum
A
Ramirez
Maria I
MI
D000818:Animals; D004064:Digestive System / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D004622:Embryo, Mammalian; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008168:Lung / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D038081:Organogenesis; D011247:Pregnancy