T-loop formation by human telomeric G-quadruplex.
T-loops were a proposed telomere structure and have been speculated to protect telomeres and regulate telomerase. In the current study, we examined the structural and thermodynamic properties of the dimeric G-quadruplex of human telomeric DNA using chemical modifications and CD spectroscopy. We demonstrated that the dimeric G-quadruplex topology induced the T-loop formation in K(+) solution.
Xu
Yan
Y
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Sato
Hiroyuki
H
Shinohara
Ken-Ichi
K
Komiyama
Makoto
M
Sugiyama
Hiroshi
H
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry; D019281:Dimerization; D054856:G-Quadruplexes; D006801:Humans; D008958:Models, Molecular; D016615:Telomere / Q000737:chemistry*; D013816:Thermodynamics
D004247:DNA