Short tandem repeat typing technologies used in human identity testing.
Short tandem repeat (STR) typing methods are widely used today for human identity testing applications including forensic DNA analysis. Following multiplex PCR amplification, DNA samples containing the length-variant STR alleles are typically separated by capillary electrophoresis and genotyped by comparison to an allelic ladder supplied with a commercial kit. This article offers a brief perspective on the technologies and issues involved in STR typing.
Butler
John M
JM
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8311, USA. john.butler@nist.gov
D001709:Biotechnology / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D053803:Forensic Genetics / Q000379:methods*; D018895:Microsatellite Repeats / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*