Positive Streptobacillus moniliformis PCR in guinea pigs likely due to Leptotrichia spp.
Streptobacillus moniliformis is a zoonotic bacterium. We obtained positive S. moniliformis PCR results in oral swab samples from guinea pigs from an experimental colony and the breeding colony of origin. Comparison of the DNA sequence of an amplicon with deposited 16S rDNA sequences revealed that Leptotrichia sp. can be the source of a false positive S. moniliformis PCR outcome.
Boot
Ron
R
Section of Laboratory Animal Microbiology, Diagnostic Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands. r.boot@rivm.nl
Van de Berg
Lia
L
Reubsaet
Frans A G
FA
Vlemminx
Maurice J
MJ
D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005189:False Positive Reactions; D005674:Fusobacterium Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D006168:Guinea Pigs / Q000382:microbiology*; D045183:Leptotrichia / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards / Q000662:veterinary*; D012376:Rodent Diseases / Q000175:diagnosis* / Q000382:microbiology; D016415:Sequence Alignment / Q000662:veterinary; D013045:Species Specificity; D013288:Streptobacillus / Q000302:isolation & purification*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal