Effect of zinc on cellular poly(ADP-ribosyl)ation capacity.
Poly(ADP-ribosyl)ation is a posttranslational protein modification, which is catalyzed by poly(ADP-ribose) polymerase-1 (PARP-1) and plays a role in DNA repair and maintenance of genomic stability. A decrease in cellular poly(ADP-ribosyl)ation has been implicated in the aging process. As PARP-1 is a zinc finger protein its decreased function might be related to age-related zinc deficiency. To test this hypothesis we assessed cellular poly(ADP-ribosyl)ation capacity in 29 donors from Greece, Italy and Poland as function of age and nutritional zinc status. Our results reveal a positive correlation between cellular poly(ADP-ribosyl)ation capacity and zinc status in human peripheral blood mononuclear cells (PBMC) (p<0.05). We could also confirm a decrease of PARP-1 activity with donor age, highlighting the role of poly(ADP-ribosyl)ation in the aging process. The results demonstrate that zinc supplementation in elderly people can increase the cellular poly(ADP-ribosyl)ation capacity of their PBMC. We speculate that this may help maintain integrity and stability of the genome more efficiently and thus contribute to an extension of healthspan.
Kunzmann
Andrea
A
Department of Biology, University of Konstanz, Konstanz, Germany.
Dedoussis
George
G
Jajte
Jolanta
J
Malavolta
Marco
M
Mocchegiani
Eugenio
E
Bürkle
Alexander
A
D000368:Aged; D000375:Aging / Q000378:metabolism*; D019587:Dietary Supplements; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D000071137:Poly (ADP-Ribose) Polymerase-1; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D014131:Trace Elements / Q000008:administration & dosage*; D015032:Zinc / Q000008:administration & dosage / Q000378:metabolism / Q000502:physiology*
D014131:Trace Elements; D011064:Poly Adenosine Diphosphate Ribose; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; D015032:Zinc