title: Biflavonoids isolated from Selaginella tamariscina regulate the expression of matrix metalloproteinase in human skin fibroblasts.
abstract: The methanol extract from Selaginella tamariscina significantly inhibited UV irradiation induced activity of matrix metalloproteinase-1 (MMP-1) in primary fibroblasts from human skin. Using the technique of bioassay-directed chromatographic separation, five biflavonoids were isolated from the ethyl acetate soluble fraction of S. tamariscina. Here, we investigated the effect of these five biflavonoids on the regulation of MMP-1 and -2 in UV irradiated cultured dermal fibroblasts from human neonatal foreskins. Among these biflavonoids, sumaflavone and amentoflavone showed significant MMP-1 inhibitory activity in primary human dermal fibroblasts after UV irradiation. The IC(50) values of sumaflavone, amentoflavone and retinoic acid, which was used as a positive control, were 0.78, 1.8, and 10microM, respectively.
authors.affiliation: R&D Center, Amore-Pacific Corporation, 314-1, Bora-dong, Kiheung-gu, Yongin-si, Kyounggi-do 449-729, South Korea.
authors.forename: Chan-Woo
authors.initials: CW
authors.lastname: Lee
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Choi
authors.forename: Han-Sung
authors.initials: HS
authors.lastname: Kim
authors.forename: Duck-Hee
authors.initials: DH
authors.lastname: Kim
authors.forename: Ih-Seop
authors.initials: IS
authors.lastname: Chang
authors.forename: Hyun Teak
authors.initials: HT
authors.lastname: Moon
authors.forename: Song-Yi
authors.initials: SY
authors.lastname: Lee
authors.forename: Won Keun
authors.initials: WK
authors.lastname: Oh
authors.forename: Eun-Rhan
authors.initials: ER
authors.lastname: Woo
mesh_terms: D000375:Aging / Q000187:drug effects / Q000528:radiation effects; D044946:Biflavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism / Q000528:radiation effects; D015394:Molecular Structure; D032503:Selaginellaceae / Q000737:chemistry*; D012867:Skin / Q000166:cytology / Q000528:radiation effects
chemical_list: D044946:Biflavonoids; D020781:Matrix Metalloproteinase 1