No association between SNP309 promoter polymorphism in the MDM2 and cervical cancer in a study from northeastern Brazil.
BACKGROUND
A functional variant in the MDM2 promoter (a T/G substitution, SNP309) was found to be associated with increased expression of MDM2 gene products and at significantly earlier age onset of tumors in both hereditary Li-Fraumeni individuals and sporadic cancer patients. We tested the hypothesis that this functional variant was associated with either risk or early age diagnosis of cervical cancer in a Brazilian population.


METHODS
A primer-introduced restriction analysis PCR assay was used to genotype the MDM2 SNP309 of 72 cervical carcinoma patients and 100 healthy women.


RESULTS
No statistically significant association was observed between SNP309 and cervical cancer. We did not find allele or genotype frequency differences between the group of patients with cancer diagnosis at an early age (younger than 40 years old) and the group of older patients.


CONCLUSIONS
Our findings suggest that SNP309 MDM2 may not be a risk factor for cervical cancer.
Meissner
Rosely de Vasconcellos
Rde V
Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Norte, Lagoa Nova, CEP:59072-970 Natal, RN, Brasil. vmrosely@yahoo.com.br
Barbosa
Rodrigo Niskier Ferreira
RN
Fernandes
José Veríssimo
JV
Galvão
Tatiana Medeiros
TM
Galvão
Ana Flávia Oliveira
AF
Oliveira
Gustavo Henrique de Medeiros
GH
D000328:Adult; D017668:Age of Onset; D001938:Brazil; D002277:Carcinoma / Q000235:genetics*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2