Diagnostic cellular organization features extracted from autofluorescence images.
Depth-resolved NADH autofluorescence images are shown to differentiate between normal and precancerous engineered tissues. An inverse power law behavior of the power spectral density (PSD) of these images is observed, indicating a self-affine organization of mitochondrial NADH at length scales 1-10 microm. Power exponents of the PSD functions vary significantly with tissue depth and precancerous state, giving insight into the morphological changes associated with precancerous lesions and providing substantial potential for noninvasive clinical diagnosis of squamous epithelial lesions and tumors.
Levitt
Jonathan M
JM
Biomedical Engineering Department, Tufts University, Medford, Massachusetts 02155, USA. Jonathan.Levitt@tufts.edu
Hunter
Martin
M
Mujat
Claudia
C
McLaughlin-Drubin
Margaret
M
Münger
Karl
K
Georgakoudi
Irene
I
D017709:Fractals; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D021962:Membrane Microdomains / Q000473:pathology; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D008928:Mitochondria / Q000473:pathology*; D009243:NAD / Q000737:chemistry*; D009369:Neoplasms / Q000473:pathology*; D018307:Neoplasms, Squamous Cell / Q000473:pathology*; D055095:Optics and Photonics; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods
D009243:NAD