Biflavonoids isolated from Selaginella tamariscina regulate the expression of matrix metalloproteinase in human skin fibroblasts.
The methanol extract from Selaginella tamariscina significantly inhibited UV irradiation induced activity of matrix metalloproteinase-1 (MMP-1) in primary fibroblasts from human skin. Using the technique of bioassay-directed chromatographic separation, five biflavonoids were isolated from the ethyl acetate soluble fraction of S. tamariscina. Here, we investigated the effect of these five biflavonoids on the regulation of MMP-1 and -2 in UV irradiated cultured dermal fibroblasts from human neonatal foreskins. Among these biflavonoids, sumaflavone and amentoflavone showed significant MMP-1 inhibitory activity in primary human dermal fibroblasts after UV irradiation. The IC(50) values of sumaflavone, amentoflavone and retinoic acid, which was used as a positive control, were 0.78, 1.8, and 10microM, respectively.
Lee
Chan-Woo
CW
R&D Center, Amore-Pacific Corporation, 314-1, Bora-dong, Kiheung-gu, Yongin-si, Kyounggi-do 449-729, South Korea.
Choi
Hyun-Jung
HJ
Kim
Han-Sung
HS
Kim
Duck-Hee
DH
Chang
Ih-Seop
IS
Moon
Hyun Teak
HT
Lee
Song-Yi
SY
Oh
Won Keun
WK
Woo
Eun-Rhan
ER
D000375:Aging / Q000187:drug effects / Q000528:radiation effects; D044946:Biflavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism / Q000528:radiation effects; D015394:Molecular Structure; D032503:Selaginellaceae / Q000737:chemistry*; D012867:Skin / Q000166:cytology / Q000528:radiation effects
D044946:Biflavonoids; D020781:Matrix Metalloproteinase 1