Effect of voluntary exercise on number and volume of cardiomyocytes and their mitochondria in the mouse left ventricle.
Voluntary exercise (VE) has a beneficial influence on the heart and mean lifespan. The present study evaluates structural adaptations of cardiomyocytes and their mitochondria due to VE by new, unbiased stereological methods. Female, 7-9-week-old mice were randomly assigned to a control (CG, n = 7) or VE group (EG, n = 7). EG animals were housed in cages with free access to a running wheel and had a mean running distance of 6.7 (1.8) km per day. After 4 weeks, the hearts of all mice were processed for light and electron microscopy. We estimated the number and volume of cardiomyocytes by the disector method and the number and volume of mitochondria by estimation of the Euler number. In comparison to CG, VE did not have an effect on the myocardial volume of the left ventricle (CG: 93 (10), EG: 103 (17) (mm(3))), the number of cardiomyocytes (CG: 2.81 (0.27), EG: 2.82 (0.43) (x10(6))) and their number-weighted mean volume. However, the composition of the cardiomyocytes changed due to VE. The total volume of mitochondria (CG: 21.8 (4.9), EG: 32.2 (4.3) (mm(3)), P < 0.01) and the total number (CG: 3.76 (0.44), EG: 7.02 (1.13) (x10(10)), P < 0.001) were significantly higher in EG than in CG. The mean number-weighted mitochondrial volume was smaller in EG than in CG (P < 0.05). In summary, VE does not alter ventricular volume nor cardiomyocyte volume or number but the oxidative capacity of cardiomyocytes by an increased mitochondrial number and total volume in the left ventricle. These structural changes may participate in the beneficial effects of VE.
Eisele
Judith Christina
JC
Department of Anatomy, University of Göttingen, Göttingen, Germany.
Schaefer
Inga-Marie
IM
Randel Nyengaard
Jens
J
Post
Heiner
H
Liebetanz
David
D
Brüel
Annemarie
A
Mühlfeld
Christian
C
D000818:Animals; D005260:Female; D006352:Heart Ventricles / Q000033:anatomy & histology / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D008929:Mitochondria, Heart / Q000502:physiology* / Q000648:ultrastructure; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D009929:Organ Size; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011897:Random Allocation; D012420:Running / Q000502:physiology; D013223:Statistics as Topic; D016276:Ventricular Function; D014836:Volition