title: pH-controlled construction of chitosan/alginate multilayer film: characterization and application for antibody immobilization.
abstract: In this work, a chitosan/alginate multilayer film was constructed via layer-by-layer self-assembly and studied by in situ surface plasmon resonance monitoring and contact angle measurements. The results demonstrate that the surface composition of the self-assembled multilayer film can be simply tailored through pH control during the assembly process. The biological property of the assembled film was further characterized via antigen-antibody interactions, showing that the loading capacity of the antibody on the multilayered film and the binding activity of the antigen to the immobilized antibody could be also well-tuned by pH control. This work can provide more scientific insight in the interaction between protein and polymer matrix and render a novel simple approach to build high-performance biointerfaces through pH control for potential applications of highly sensitive immunosensors.
authors.affiliation: School of Chemical & Biomedical Engineering, Center for Advanced Bionanosystems, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457.
authors.forename: Weiyong
authors.initials: W
authors.lastname: Yuan
authors.forename: Hua
authors.initials: H
authors.lastname: Dong
authors.forename: Chang Ming
authors.initials: CM
authors.lastname: Li
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Cui
authors.forename: Ling
authors.initials: L
authors.lastname: Yu
authors.forename: Zhisong
authors.initials: Z
authors.lastname: Lu
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D000936:Antigen-Antibody Complex; D048271:Chitosan / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D018625:Microscopy, Atomic Force; D020349:Surface Plasmon Resonance
chemical_list: D000464:Alginates; D000906:Antibodies; D000936:Antigen-Antibody Complex; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D048271:Chitosan