Immunogenicity of Helicobacter pylori urease B protein and DNA vaccines in a mouse model.
A vaccine could alleviate major morbidity and mortality associated with Helicobacter pylori infection. We immunized BALB/c mice with 3 doses of a protein or DNA vaccine based on H pylori urease B. Protein alone was immunogenic even after the first dose, whereas DNA did not elicit antibodies after 3 doses. DNA preceding protein (D-P-P) appeared to blunt the response to protein, whereas DNA following protein (P-D-D) shifted from a predominantly T helper 2 (Th2) profile to a balanced Th1:Th2 profile. These preliminary findings may have important implications for the development of an H pylori vaccine.
Bégué
Rodolfo E
RE
Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans, USA. rbegue@lsuhsc.edu
Cruz
Alma R
AR
Ramgoolam
Andres
A
Breslin
Mary B
MB
D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology / Q000627:therapeutic use*; D001428:Bacterial Vaccines / Q000276:immunology / Q000627:therapeutic use*; D004247:DNA / Q000276:immunology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016481:Helicobacter Infections / Q000517:prevention & control; D016480:Helicobacter pylori / Q000201:enzymology* / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D014510:Urease / Q000276:immunology*; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000276:immunology / Q000627:therapeutic use*
D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D004247:DNA; D014510:Urease