title: Structural characterization of supercoiled DNA containing a minisatellite repeat that has polypurine/polypyrimidine stretch.
abstract: The pUC19 derivative pAL79 contains six tandem repeats of a 30-bp unit sequence. Supercoiled pAL79 molecules show aberrant electrophoretic mobility under acidic conditions, and microscopic analysis suggests pAL79 molecules conform to tight plectonemes when deposited on a substrate under acidic conditions. This may mean that a short inserted DNA sequence affects the global topology of plasmid DNA.
authors.affiliation: Department of Biological Science, School of Science, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, Sakai 599-8531 Japan. mkato@b.s.osakafu-u.ac.jp
authors.forename: Mikio
authors.initials: M
authors.lastname: Kato
authors.forename: Takeshi
authors.initials: T
authors.lastname: Haku
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamauchi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sugiyama
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Nagayama
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Shimizu
mesh_terms: D004278:DNA, Superhelical / Q000737:chemistry* / Q000648:ultrastructure; D015180:Electrophoresis, Gel, Two-Dimensional; D018598:Minisatellite Repeats; D010957:Plasmids / Q000737:chemistry; D011687:Purines / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry
chemical_list: D004278:DNA, Superhelical; D011687:Purines; D011743:Pyrimidines