[Inhibitory effect of LRRC4 on the mobility and invasion of glioblastomas through the SDF-1alpha/CXCR4 axis].
OBJECTIVE
To explore the effect of LRRC4 on the mobility and invasion of glioblastomas U251 cells through the SDF-1alpha/CXCR4 axis.


METHODS
RT-PCR, transfilter cell invasion assay, adhesion assay, scraping test, scrape loading, and dye transfer assay were used to determine the effect of LRRC4 on U251 cells.


RESULTS
SDF-1 alpha could increase the invasion in U251 which expressed CXCR4. The reintroduction of LRRC4 in U251 cells could inhibit the expression of CXCR4. LRRC4 also inhibited the adhesion ability of U251 to ECV304 as well as the mobility and invasion ability in vitro, which was mediated by the SDF-1alpha/CXCR4 axis. Furthermore, LRRC4 could greatly enhance the gap junctional intercellular communication of U251 cells.


CONCLUSIONS
The reintroduction of LRRC4 in U251 cells can inhibit the expression of CXCR4 and the SDF-1alpha/CXCR4 axis-mediated cell invasion in vitro.
Chen
Qiong
Q
Cancer Research Institute, Central South University, Changsha 410078, China.
Wu
Ming-hua
MH
Zhou
Yan-hong
YH
Tang
Yun-Lian
YL
Huang
Chen
C
Li
Xiao-ling
XL
Li
Gui-yuan
GY
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D054377:Chemokine CXCL12 / Q000378:metabolism*; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D009361:Neoplasm Invasiveness; D009419:Nerve Tissue Proteins / Q000235:genetics*; D019718:Receptors, CXCR4 / Q000378:metabolism*
C515255:CXCL12 protein, human; C527276:CXCR4 protein, human; D054377:Chemokine CXCL12; C503331:LRRC4 protein, human; D009419:Nerve Tissue Proteins; D019718:Receptors, CXCR4