Spectroscopic studies on a novel intramolecular hydrogen bond within the (6-4) photoproduct.
The (6-4) photoproduct, which is one of major UV-induced lesions formed between adjacent pyrimidine bases in DNA, was characterized by using (15)N NMR, fluorescence emission, and UV/VIS absorption at various pH values. From these experiments, we identified a novel intramolecular hydrogen bond between the hydroxyl group at the C5 position of the 5' component and the N3 of the 3' pyrimidone ring within the (6-4) photoproduct.
Yamamoto
Junpei
J
Division of Chemistry, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan. yamagen@chembio.chem.es.osaka-u.ac.jp
Tanaka
Yoshiyuki
Y
Hitomi
Kenichi
K
Getzoff
Elizabeth D
ED
Iwai
Shigenori
S
D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D019906:Nuclear Magnetic Resonance, Biomolecular; D011740:Pyrimidine Dimers / Q000737:chemistry*; D011741:Pyrimidine Nucleosides / Q000737:chemistry
C528345:5-methylpyrimidine-2-one 2'-deoxyribonucleoside; D011740:Pyrimidine Dimers; D011741:Pyrimidine Nucleosides; C058406:pyrimidine-pyrimidone dimer