title: Myocardium at the base of the aorta and pulmonary trunk is prefigured in the outflow tract of the heart and in subdomains of the second heart field.
abstract: Outflow tract myocardium in the mouse heart is derived from the anterior heart field, a subdomain of the second heart field. We have recently characterized a transgene (y96-Myf5-nlacZ-16), which is expressed in the inferior wall of the outflow tract and then predominantly in myocardium at the base of the pulmonary trunk. Transgene A17-Myf5-nlacZ-T55 is expressed in the developing heart in a complementary pattern to y96-Myf5-nlacZ-16, in the superior wall of the outflow tract at E10.5 and in myocardium at the base of the aorta at E14.5. At E9.5, the two transgenes are transcribed in different subdomains of the anterior heart field. A clonal analysis of cardiomyocytes in the outflow tract, at E10.5 and E14.5, provides insight into the behaviour of myocardial cells and their progenitors. At E14.5, most clones are located at the base of either the pulmonary trunk or the aorta, indicating that these derive from distinct myocardial domains. At E10.5, clones are observed in subdomains of the outflow tract. The distribution of small clones indicates proliferative differences, whereas regionalization of large clones, that derive from an early myocardial progenitor cell, reflect coherent cell growth in the heart field as well as in the myocardium. Our results suggest that myocardial differences at the base of the great arteries are prefigured in distinct progenitor cell populations in the anterior heart field, with important implications for understanding the etiology of congenital heart defects affecting the arterial pole of the heart.
authors.affiliation: Department of Developmental Biology, URA 2578 CNRS, Pasteur Institute, 25 rue du Dr Roux, 75724 Paris, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Bajolle
authors.forename: Stéphane
authors.initials: S
authors.lastname: Zaffran
authors.forename: Sigolène M
authors.initials: SM
authors.lastname: Meilhac
authors.forename: Mathieu
authors.initials: M
authors.lastname: Dandonneau
authors.forename: Ted
authors.initials: T
authors.lastname: Chang
authors.forename: Robert G
authors.initials: RG
authors.lastname: Kelly
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Buckingham
mesh_terms: D000818:Animals; D001011:Aorta / Q000196:embryology; D006321:Heart / Q000196:embryology*; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D008822:Mice, Transgenic; D009206:Myocardium / Q000166:cytology*; D051787:Myogenic Regulatory Factor 5 / Q000235:genetics; D013234:Stem Cells / Q000166:cytology
chemical_list: C497418:Myf5 protein, mouse; D051787:Myogenic Regulatory Factor 5