Protective effects of a freeze-dried extract of vegetables and fruits on the hydroxyl radical-mediated oxidative damage of DNA and decrease of erythrocytes deformability.
The protective effects of a freeze-dried extracts of vegetables and fruits (BauYuan; BY) on the hydroxyl radical-mediated DNA strand breakages and the structural integrity of human red blood cells (RBCs) were investigated. First, the supercoiled plasmid (pEGFP-C1) DNA was subjected to oxidation damage by an ascorbate-fortified Fenton reaction and the protective effects were analyzed by agarose gel electrophoresis. In the absence of BY extracts, exposure of the high-throughput .OH-generating system (Fe2+ concentration >1.0 microM) caused a complete fragmentation of DNA. Supplementation of BY extract (1 mg/mL) to the plasmid DNA prior to the exposure could prevent it significantly. In contrast, as the plasmid exposed to a low-grade .OH-generating system (Fe2+<0.1 microM), the BY extract (1 mg/mL) provided an almost complete protection. Next, the cell deformabilities were measured to assess the protection effects of various BY extracts on human erythrocytes exposed to the oxidative insults. We found that both the aqueous extract and the organic solvent-derived extracts could strongly protect human RBCs from the reactive oxygen species (ROS)-mediated decrease in the deformability indices. The results implicated that the BY extracts could effectively protect the cell membrane integrity via scavenging ROS which enabling RBCs to maintain a balance of water content and surface area to prevent the drop of cell deformability.
Wang
Hsiao-Ning
HN
Graduate Institute of Medical Biotechnology, Chang-Gung University, Taoyuan, Taiwan.
Liu
Tsan-Zon
TZ
Chen
Ya-Lei
YL
Shiuan
David
D
D000818:Animals; D004247:DNA / Q000737:chemistry; D004249:DNA Damage; D004907:Erythrocyte Deformability; D004912:Erythrocytes / Q000378:metabolism; D005609:Free Radicals; D005612:Freeze Drying; D005638:Fruit; D006801:Humans; D017665:Hydroxyl Radical; D007501:Iron / Q000737:chemistry; D051379:Mice; D041681:NIH 3T3 Cells; D009994:Osmolar Concentration; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D014675:Vegetables
D005609:Free Radicals; D017665:Hydroxyl Radical; D004247:DNA; D007501:Iron; D010100:Oxygen