[Isolation of surface antigen of hepatites B virus].
The scheme of immunoaffinity isolation of surface antigen of hepatitis B virus is developed. Selection of monoclonal antibodies was carried out and conditions for synthesis of immunoaffinity matrix were optimized; specificity of binding of HBs-antigen with a sorbent is improved; conditions of washing of columns and elution of HBs-antigen are chosen. The yield of purified HBs-antigen obtained according to the developed scheme exceeded 90% and it allows to use such HBsAg for immunization of animals and application in immunoanalysis.
Nikolaenko
I V
IV
Goncharenko
V S
VS
Shimko
N N
NN
Galkin
A Iu
AIu
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D006510:Hepatitis B Antibodies / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000276:immunology / Q000302:isolation & purification*; D017325:Hepatitis B Vaccines / Q000276:immunology; D006515:Hepatitis B virus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines