Intermolecular transfer of aminoacylated adenosine towards chemical aminoacylation of tRNA.
We synthesized two oligonucleotides: one is DNA conjugated with adenosine derivative at 5' terminus as a donor, another RNA phosphorothioated at 3' terminus as an acceptor. By hybridization of the two oligonuleotides, the adenosine derivative was transferred from the donor DNA to the acceptor RNA. In this study, O, O'-isopropylidene-protected adenosine derivative and aminoacylated adenosine were successfully transferred from the donor to acceptor RNA. By use of tRNA instead of the acceptor RNA, the chemical aminoacylation of tRNA by unnatural amino acid is expected.
Liu
Mingzhe
M
Nano Medical Engineering Laboratory, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan.
Jinmei
Hiroshi
H
Fukuzono
Koji
K
Abe
Hiroshi
H
Ito
Yoshihiro
Y
D000241:Adenosine / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D012343:RNA, Transfer / Q000737:chemistry*; D046249:Transfer RNA Aminoacylation
D009841:Oligonucleotides; D012313:RNA; D004247:DNA; D012343:RNA, Transfer; D000241:Adenosine