Comparatine analysis of cytophenotypes of cells of mesenchymal lineage isolated from human tissues.
The expression of cytoplasmic and surface proteins in cultured human skin fibroblasts, human umbilical cord cells obtained after normal delivery on gestation week 38-40, and mesenchymal bone marrow stem cells was compared by the methods of immunocytochemistry and flow cytofluorometry. Bone marrow mesenchymal stem cells expressed a great variety of marker proteins typical of stem and progenitor cells and did not express proteins typical of differentiated cells. Fibroblast-like umbilical cord cells expressed markers of both stem cells and differentiated cells. Fibroblasts of dermal origin were characterized by intensive expression of proteins typical of differentiated cells.
Suzdal'tseva
Yu G
YG
Russian State Medical University, Moscow.
Burunova
V V
VV
Petrakova
N V
NV
Vakhrushev
I V
IV
Yarygin
K N
KN
Yarygin
V N
VN
D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D019070:Cell Lineage; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology*; D020543:Proteome / Q000096:biosynthesis; D012867:Skin / Q000166:cytology; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology*
D020543:Proteome