Evidence for a new species of Leishmania associated with a focal disease outbreak in Ghana.
A previously unknown Leishmania spp., inferred by DNA sequence analysis of the small subunit ribosomal RNA gene and the internal transcribed spacer region (ITS1), was detected in tissue biopsies from patients living in the Eastern Ghanaian community of Taviefe. Restriction fragment length polymorphism analysis of the ITS1 amplicon supports the possibility of an uncharacterized Leishmania spp.
Villinski
Jeffrey T
JT
US Naval Medical Research Unit No. 3, Cairo, Egypt.
Klena
John D
JD
Abbassy
Magda
M
Hoel
David F
DF
Puplampu
Naiki
N
Mechta
Sarah
S
Boakye
Daniel
D
Raczniak
Gregory
G
D000818:Animals; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks; D005869:Ghana / Q000453:epidemiology; D006801:Humans; D007891:Leishmania / Q000145:classification* / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000453:epidemiology* / Q000469:parasitology*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D021901:DNA, Intergenic; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S