[Protective effect of ischemic preconditioning against cold ischemia and reperfusion injury of rat small intestinal graft].
OBJECTIVE
To evaluate the protective effect of ischemic preconditioning against cold ischemia and reperfusion injury of rat intestinal graft following orthotopic transplantation.


METHODS
Eighty SD rats were randomly assigned into two groups with and without ischemic preconditioning, and each group was divided into 4 subgroups (n=10) according to the intestinal graft cold ischemia time of 3, 6, 12, and 18 h, respectively. Ischemic preconditioning model was established, and the small intestinal graft was preserved at 4 degrees celsius; in Ringer lactate solution for the corresponding time, followed by orthotopic transplantation of the graft. The graft samples were collected for histological examination 1 h after reperfusion, and nuclear factor-kappaB (NF-kappaB) expression in the epithelial cells was detected.


RESULTS
Ischemia preconditioning obviously relieved the histological ischemia/reperfusion injury, as shown by regular alignment of the small intestinal villi, alleviated muscular layer edema and decreased expression of NF-kappaB in the epithelia of the graft in groups with cold preservation.


CONCLUSIONS
Ischemic preconditioning can protect the intestinal graft from cold ischemia/reperfusion injury, and NF-kappaB is an important cytokine in ischemia preconditioning.
Zhao
Wei
W
Department of General Surgery, First Affiliated Hospital of School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
Che
Xiang-ming
XM
Fan
Lin
L
Wang
Shu-feng
SF
Wang
Guang-hui
GH
Zhang
Ru-yuan
RY
Zhang
Li
L
Zhang
Feng
F
D000818:Animals; D050377:Cold Ischemia; D007421:Intestine, Small / Q000473:pathology / Q000637:transplantation*; D019194:Ischemic Preconditioning; D016328:NF-kappa B / Q000378:metabolism; D009926:Organ Preservation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*
D016328:NF-kappa B