title: Oligonucleotide transformation for the study of mutagenic specificities of DNA lesions in yeast.
abstract: We developed a method for the analyzing mutagenic potential of DNA damage based on the oligonucleotide transformation technique in yeast. Using this assay we have analyzed mutagenic specificities of various DNA lesions. In the present study, we analyzed the mutagenic properties of 2-hydroxyadenine and 5-hydroxycytosine in yeast. Oligonucleotides containing 2-hydroxyadenine or 5-hydroxycytosine were used for the transformation. The oligonucleotides showed transforming activities similar to unmodified oligonucleotides. This indicates that no repair systems were working on them. The sequencing data of the transformants showed that 5-hydroxycytosine and 2-hydroxyadenine are read mainly as cytosine and adenine. We will also discuss the mechanism of oligonucleotide transformation and its application to the mutagenesis study.
authors.affiliation: Nihon Pharmaceutical University, 10281 Komuro, Ina, Saitama 362-0806, Japan. knegishi@nichiyaku.ac.jp
authors.forename: Kazuo
authors.initials: K
authors.lastname: Negishi
authors.forename: Daijiro
authors.initials: D
authors.lastname: Sekine
authors.forename: Takumi
authors.initials: T
authors.lastname: Morimitsu
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Suzuki
authors.forename: Yoji
authors.initials: Y
authors.lastname: Okugawa
authors.forename: Asako
authors.initials: A
authors.lastname: Kawakami
authors.forename: Chie
authors.initials: C
authors.lastname: Otsuka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Oyama
authors.forename: David
authors.initials: D
authors.lastname: Loakes
mesh_terms: D003596:Cytosine / Q000031:analogs & derivatives* / Q000737:chemistry; D004249:DNA Damage; D004252:DNA Mutational Analysis; D006147:Guanine / Q000737:chemistry*; D016296:Mutagenesis; D009841:Oligonucleotides / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D009841:Oligonucleotides; D006147:Guanine; C017400:5-hydroxycytosine; D003596:Cytosine; C008183:isoguanine