[Intrasplenic tumor model of nude mice in the anti- metastasis roles of NGX6 gene against colon cancer].
OBJECTIVE
To establish a liver metastasis model of nude mice in colon cancer so as to determine the function of NGX6.


METHODS
The cells of Group HT-29, pcDNA3.1(+)/HT-29, and pcDNA3.1(+)/NGX6/HT-29 were implanted into the spleen of nude mice, respectively. Everyday we measured the weight of the nude mice and observed their ingestion, movement and mental status. The nude mice were killed after 45 days, and the effect of NGX6 on the malignant behavior of HT-29 was assessed by this experiment.


RESULTS
In contrast to the other two groups, the metastasis in the liver and xenograft tumor in the spleen of pcDNA3.1(+)/NGX6/HT-29 group was significantly reduced (P<0.01).


CONCLUSIONS
The metastasis of HT-29 colon cancer cell line was significantly inhibited by NGX6 gene. This model of liver metastasis in the nude mice is a proper model to determine the anti-metastasis mechanism of NGX6 gene.
Xiao
Zhi-ming
ZM
Department of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Shen
Shou-rong
SR
Lian
Ping
P
Wang
Xiao-yan
XY
Liu
Fen
F
D000818:Animals; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology*; D004195:Disease Models, Animal; D015972:Gene Expression Regulation, Neoplastic; D019073:HT29 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000556:secondary*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D013160:Splenic Neoplasms / Q000473:pathology; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D008565:Membrane Proteins; C477325:TMEM8B protein, human; D025521:Tumor Suppressor Proteins