The ectonucleotidases alkaline phosphatase and nucleoside triphosphate diphosphohydrolase 2 are associated with subsets of progenitor cell populations in the mouse embryonic, postnatal and adult neurogenic zones.
Subventricular zone (SVZ)-derived adult neurospheres express two ectonucleotidases, nucleoside triphosphate diphosphohydrolase 2 (NTPDase2) and tissue non-specific alkaline phosphatase (TNAP). Agonists of the nucleotide receptors P2Y(1) and P2Y(2) as well as adenosine augment growth factor-mediated progenitor cell proliferation. NTPDase2 converts ATP and UTP to ADP and UDP, respectively, which are all P2Y receptor agonists. TNAP hydrolyzes nucleoside triphosphates and diphosphates and produces the P1 receptor agonist adenosine. In the SVZ, NTPDase2 is specifically expressed by type B cells. In order to further scrutinize the association of key molecules of the purinergic signaling pathway with neurogenic regions, we analyzed the expression of TNAP at the lateral ventricles of the adult and developing mouse brain. In the adult brain, TNAP was expressed by type B, type A and at least subsets of type C cells of the SVZ and throughout the rostral migratory stream. Almost 100% of the proliferating, Ki-67-positive cells of the adult SVZ stained for TNAP, supporting the notion of a ubiquitous association of TNAP with SVZ progenitors. In contrast, NTPDase2-positive progenitors of the dentate gyrus were TNAP-negative. Essentially all cells of the telencephalic vesicle at embryonic day (E) 14 revealed TNAP activity, including doublecortin-positive neuroblasts. During further embryonic development, enhanced TNAP activity became restricted to cells of the ventricular and SVZ. In contrast to TNAP, NTPDase2 was first expressed in the SVZ perinatally, in association with TNAP-positive SVZ border cells. During later development, NTPDase2-positive cells disappeared from the ventricular surface and began to form sheaths around clusters of subventricular doublecortin-positive cells, apparently transforming into type B cells. Our results identify TNAP and NTPDase2 as novel markers for subsets of progenitors in the adult and developing mouse brain. They further support the notion that signaling via extracellular nucleotides and nucleosides contributes to embryonic and adult neurogenesis.
Langer
D
D
Institute of Cell Biology and Neuroscience, Biocenter, J.W. Goethe-University, Max-von Laue-Str. 9, 60438 Frankfurt am Main, Germany.
Ikehara
Y
Y
Takebayashi
H
H
Hawkes
R
R
Zimmermann
H
H
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D000089622:Doublecortin Domain Proteins; D004622:Embryo, Mammalian; D027341:Excitatory Amino Acid Transporter 1; D005260:Female; D018507:Gene Expression Regulation, Developmental; D019394:Ki-67 Antigen / Q000378:metabolism; D020547:Lateral Ventricles / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism; D043583:Nucleoside-Triphosphatase / Q000378:metabolism*; D011247:Pregnancy; D013234:Stem Cells / Q000502:physiology*
D000089622:Doublecortin Domain Proteins; D027341:Excitatory Amino Acid Transporter 1; D019394:Ki-67 Antigen; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; C493393:Slc1a3 protein, mouse; C071871:zebrin II; D000469:Alkaline Phosphatase; D000251:Adenosine Triphosphatases; C027201:ectoATPase; D043583:Nucleoside-Triphosphatase