Cooperative regulation of p73 promoter by Yin Yang 1 and E2F1.
The transcriptional factor Yin Yang 1 (YY1) plays multifunctional role in various biological processes. Here we report that the silencing of YY1 using siRNA expression vectors in U2OS cells led to a significant decrease in transcriptional activity of p73, which is a member of p53 family. Consistently, overexpression of YY1 could enhance the transcriptional activity of p73 in a dose-dependent manner. Moreover, synergistic cooperation between YY1 and E2F1, through a mechanism involving a physical interaction, was observed in the regulation of p73 promoter. In conclusion, our results provide new insights into the function of YY1, and might clarify the complex regulation mechanism by YY1 network in diverse biological processed, such as development, differentiation and cancer biology.
Wu
Shourong
S
Department of Chemistry and Biotechnology, Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Murai
Saomi
S
Kataoka
Kazunori
K
Miyagishi
Makoto
M
D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics*; D050687:E2F1 Transcription Factor / Q000378:metabolism*; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D015533:Transcriptional Activation; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D050986:YY1 Transcription Factor / Q000037:antagonists & inhibitors / Q000378:metabolism*
D004268:DNA-Binding Proteins; D050687:E2F1 Transcription Factor; D009687:Nuclear Proteins; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins; D050986:YY1 Transcription Factor