Modulation of Congo-red-induced aberrations in the yeast Saccharomyces cerevisiae by the general stress response protein Hsp12p.
Previous studies have shown that in Saccharomyces cerevisiae HSP12, which codes for the small cell wall heat shock protein Hsp12p, was induced upon exposure to cell-wall-damaging agents such as Congo red. Here, we demonstrate that Hsp12p decreases the interaction between Congo red and chitin. A Deltahsp12 mutant strain displayed decreased viability, increased aggregation and sedimentation, as well as an altered morphology when grown in the presence of Congo red dye. The presence of Hsp12p was also necessary for the Congo-red-mediated invasion of agar plates.
Karreman
Robert J
RJ
Department of Molecular and Cell Biology, University of Cape Town, Private Bag 7725, Rondebosch, South Africa.
Lindsey
George G
GG
D002473:Cell Wall / Q000378:metabolism*; D002686:Chitin / Q000378:metabolism*; D003224:Congo Red / Q000494:pharmacology*; D015966:Gene Expression Regulation, Fungal; D006360:Heat-Shock Proteins / Q000378:metabolism / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*
C112591:HSP12 protein, S cerevisiae; D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; D002686:Chitin; D003224:Congo Red