Genomic analysis of gene expression of Staphylococcus aureus.
The microarray has shown tremendous potential for investigating gene expression profiles and expression levels in comparative biology; exploring the regulation mechanisms of gene expression; and evaluating target gene for developing new chemotherapeutic agents, vaccine, and diagnostic methods. In this chapter, we provide a detailed protocol for scientists who wish to investigate gene expression profiles by performing a microarray analysis, including different methods of RNA purification, decontamination, cDNA synthesis, fragmentation, and biotin labeling for hybridization using Affymetrix Staphylococcus aureus chips.
Yu
Chuanxin
C
Department of Veterinary and Biomedical Sciences, University of Minnesota, St Paul, MN, USA.
Sun
Junsong
J
Zheng
Li
L
Ji
Yinduo
Y
D004269:DNA, Bacterial / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013211:Staphylococcus aureus / Q000235:genetics*
D004269:DNA, Bacterial; D018076:DNA, Complementary; D012329:RNA, Bacterial