Measuring secretion in chromaffin cells using electrophysiological and electrochemical methods.
Our present understanding of exocytosis of catecholamines has benefited tremendously from the arrival of single-cell electrochemical methods (amperometry and voltammetry), electrophysiological techniques (whole-cell and patch capacitance) and from the combination of both techniques (patch amperometry). In this brief review, we will outline the strengths and limitations of amperometric and electrophysiological methods and highlight the major contribution obtained with the use of these techniques in chromaffin cells.
Borges
R
R
Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain. rborges@ull.es
Camacho
M
M
Gillis
K D
KD
D000818:Animals; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000378:metabolism*; D002837:Chromaffin Granules / Q000502:physiology; D004594:Electrophysiology; D005089:Exocytosis; D006801:Humans; D018408:Patch-Clamp Techniques