Gain of 5p15.33 is associated with progression of bladder cancer.
OBJECTIVE
To search for a biological marker to distinguish low-risk from high-risk bladder cancer indicating disease progression.


METHODS
The whole genome-wide copy numbers were screened in 18 patients with bladder cancer using array comparative genomic hybridization (CGH) consisting of 4,030 bacterial artificial chromosome clones.


RESULTS
Gain of 5p15.33, including TPPP (tubulin polymerization-promoting protein)and ZDHHC11 (zinc finger DHHC domain-containing protein 11) genes, was detected in 5 of 9 (55.6%) high-grade bladder cancers and no (0%; n = 9) low-grade bladder cancer. To confirm the preliminary data, 5p15.33 gain was studied by fluorescence in situhybridization (FISH) in 100 patients, and the results were compared with biological characteristics. In FISH analysis, gain of 5p15.33 was significantly correlated with higher histological grade (p < 0.0001) and advanced pathological stage (p = 0.0284). Tumors with a gain of 5p15.33 had a significantly higher progression-free survival rate than those without (p = 0.0006, log-rank test). Multivariate analysis revealed that gain of 5p15.33 was a predictor for disease progression in bladder cancer (hazard ratio: 1.887, 95% confidence interval: 1.215-2.968, p = 0.0050).


CONCLUSIONS
These data suggest that gain of 5p15.33 (TPPP and ZDHHC11) may become a potential biomarker identifying high-risk patients with disease progression in bladder cancer.
Yamamoto
Yoshiaki
Y
Department of Urology, Yamaguchi University School of Medicine, Ube, Japan.
Chochi
Yasuyo
Y
Matsuyama
Hideyasu
H
Eguchi
Satoshi
S
Kawauchi
Shigeto
S
Furuya
Tomoko
T
Oga
Atsunori
A
Kang
Jason J
JJ
Naito
Katsusuke
K
Sasaki
Kohsuke
K
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D022202:Chromosomes, Artificial, Bacterial; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics*; D018450:Disease Progression; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000235:genetics; D018570:Risk Assessment; D016019:Survival Analysis; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology*
D009419:Nerve Tissue Proteins; C118136:TPPP protein, human