[Effects of processing and extracting methods on active components in Radix Scutellariae by HPLC analysis].
OBJECTIVE
To compare and analyze the effects of the contents of the active components in Radix Scutellariae by different processing and extracting methods.


METHODS
The raw and processed Radix Scutellariae were used, and the concentration of baicalin, wogonoside, baicalein, wogonin were determined by HPLC after five extracting techniques. HPLC method was performed on methanol-acetonitrile-water-acetic acid (18: 25: 57: 0.2) as the mobile phase at 30 C; The chromatographic column was Agilent Zorbax Extend-C18 (4.6 mm x 250 mm, 5 microm); The flow rate was 0.8 mL min(-1), and the detection wavelength was 275 nm.


RESULTS
the ratios of baicalein and wogonin in raw material were 5.41%, 1.30% respectively by enzymatic extraction, which is higher than other extracting methods by raw material; The ratios of baicalin and wogonoside in raw material were 10.11% and 3.55% by ethanol of 60%, which is higher than other extracting methods; Different extracting methods have no evident effects on processed materials, enzymatic extraction is the best. The ratio of baicalin, wogonoside and baicalein, wogonin is 10.63%, 3.60% and 1.54%, 0.59%.


CONCLUSIONS
Different methods have evident extracting effects on the four active components with raw material, but have no evident effects with processed material. According to different active components most suitable extracting method should be adopted.
Wang
Hong-zhi
HZ
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Yu
Chun-hao
CH
Gao
Jun
J
Zhao
Guang-rong
GR
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044950:Flavanones / Q000032:analysis / Q000302:isolation & purification; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification; D006358:Hot Temperature; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D031364:Scutellaria baicalensis / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*
D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; C038044:baicalin; C006680:baicalein; C473995:wogonoside; C085514:wogonin