title: An efficient method to express GPI-anchor proteins in insect cells.
abstract: Glycosylphosphatidylinositols (GPIs) constitute a class of glycolipids that have various functions, the most basic being to attach proteins to the surface of eukaryotic cells. GPIs have to be taken into account, when expressing surface antigens from parasitic protozoa in heterologous systems. The synthesis of the GPI-anchors was previously reported to be drastically decreased to almost background level following baculovirus infection. Here we describe a new method to express GPI-anchor proteins in insect cells relying on using of a supplementary baculovirus construct that overexpresses the N-acetylglucosaminyl phosphatidylinositol de-N-acetylase, the enzyme catalyzing the second step in the GPI biosynthetic pathway.
authors.affiliation: Medizinisches Zentrum für Hygiene und Medizinische Mikrobiologie, Philipps-Universität Marburg, Hans-Meerwein-Str. 2, 35043 Marburg, Germany.
authors.forename: Hosam
authors.initials: H
authors.lastname: Shams-Eldin
authors.forename: Nahid
authors.initials: N
authors.lastname: Azzouz
authors.forename: Sebastian
authors.initials: S
authors.lastname: Niehus
authors.forename: Terry K
authors.initials: TK
authors.lastname: Smith
authors.forename: Ralph T
authors.initials: RT
authors.lastname: Schwarz
mesh_terms: D000581:Amidohydrolases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D005822:Genetic Vectors / Q000235:genetics; D017261:Glycosylphosphatidylinositols / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D018411:Spodoptera / Q000235:genetics / Q000378:metabolism*; D014162:Transfection / Q000379:methods
chemical_list: D017261:Glycosylphosphatidylinositols; D011994:Recombinant Proteins; D000581:Amidohydrolases; C087696:N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase