title: [Multi-residue method for determination of veterinary drugs and feed additives in meats by HPLC].
abstract: A simple and rapid multi-residue method was developed for the determination of 28 kinds of veterinary drugs and feed additives (drugs) in muscle of cattle, pig and chicken. The drugs were extracted with acetonitrile-water (95:5) in a homogenizer and ultrasonic generator. The extracted solution was poured into an alumina column and the drugs were eluted with acetonitrile-water (90:10). The eluate was washed with n-hexane saturated with acetonitrile and then evaporated. The drugs were separated on a Inertsil ODS-3V column (4.6 mm i.d. x 250 mm) with a gradient system of 0.1% phosphoric acid-acetonitrile as the mobile phase, with monitoring at 280 and 340 nm. The recoveries of the 26 kinds of drugs were over 60% from the meats fortified at 0.1 microg/g, and the quantification limits of most drugs were 0.01 microg/g. This proposed method was found to be effective and suitable for the screening of the above drugs in meats.
authors.affiliation: Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo 060-0819, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Chonan
authors.forename: Toru
authors.initials: T
authors.lastname: Fujimoto
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Ueno
authors.forename: Teijiro
authors.initials: T
authors.lastname: Tazawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005503:Food Additives / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D013552:Swine; D019155:Veterinary Drugs / Q000032:analysis*
chemical_list: D005503:Food Additives; D019155:Veterinary Drugs