title: Synergistic enhancement of cellobiohydrolase performance on pretreated corn stover by addition of xylanase and esterase activities.
abstract: Significant increases in the depolymerization of corn stover cellulose by cellobiohydrolase I (Cel7A) from Trichoderma reesei were observed using small quantities of non-cellulolytic cell wall-degrading enzymes. Purified endoxylanase (XynA), ferulic acid esterase (FaeA), and acetyl xylan esterase (Axe1) all enhanced Cel7A performance on corn stover subjected to hot water pretreatment. In all cases, the addition of these activities improved the effectiveness of the enzymatic hydrolysis in terms of the quantity of cellulose converted per milligram of total protein. Improvement in cellobiose release by the addition of the non-cellulolytic enzymes ranged from a 13-84% increase over Cel7A alone. The most effective combinations included the addition of both XynA and Axe1, which synergistically enhance xylan conversions resulting in additional synergistic improvements in glucan conversion. Additionally, we note a direct relationship between enzymatic xylan removal in the presence of XynA and the enhancement of cellulose hydrolysis by Cel7A.
authors.affiliation: National Renewable Energy Laboratory, Chemical and BioSciences Center, 1617 Cole Boulevard, Golden, CO 80401, United States. michael_selig@nrel.gov
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Selig
authors.forename: Eric P
authors.initials: EP
authors.lastname: Knoshaug
authors.forename: William S
authors.initials: WS
authors.lastname: Adney
authors.forename: Michael E
authors.initials: ME
authors.lastname: Himmel
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Decker
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D002475:Cellobiose / Q000378:metabolism; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000302:isolation & purification / Q000378:metabolism*; D004950:Esterases / Q000302:isolation & purification / Q000378:metabolism*; D006868:Hydrolysis; D013696:Temperature; D014242:Trichoderma / Q000201:enzymology*; D014867:Water; D014990:Xylans / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism*
chemical_list: D014990:Xylans; D014867:Water; D002475:Cellobiose; D004950:Esterases; D043364:Endo-1,4-beta Xylanases; D043366:Cellulose 1,4-beta-Cellobiosidase