title: Gamma-herpesvirus kinase actively initiates a DNA damage response by inducing phosphorylation of H2AX to foster viral replication.
abstract: DNA virus infection can elicit the DNA damage response in host cells, including ATM kinase activation and H2AX phosphorylation. This is considered to be the host cell response to replicating viral DNA. In contrast, we show that during infection of macrophages murine gamma-herpesvirus 68 (gammaHV68) actively induces H2AX phosphorylation by expressing a viral kinase (orf36). GammaHV68-encoded orf36 kinase and its EBV homolog, BGLF4, induce H2AX phosphorylation independently of other viral genes. The process requires the kinase domain of Orf36 and is enhanced by ATM. Orf36 is important for gammaHV68 replication in infected animals, and orf36, H2AX, and ATM are all critical for efficient gammaHV68 replication in primary macrophages. Thus, activation of proximal components of the DNA damage signaling response is an active viral kinase-driven strategy required for efficient gamma-herpesvirus replication.
authors.affiliation: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: Vera L
authors.initials: VL
authors.lastname: Tarakanova
authors.forename: Van
authors.initials: V
authors.lastname: Leung-Pineda
authors.forename: Seungmin
authors.initials: S
authors.lastname: Hwang
authors.forename: Chiao-Wen
authors.initials: CW
authors.lastname: Yang
authors.forename: Katie
authors.initials: K
authors.lastname: Matatall
authors.forename: Mickael
authors.initials: M
authors.lastname: Basson
authors.forename: Ren
authors.initials: R
authors.lastname: Sun
authors.forename: Helen
authors.initials: H
authors.lastname: Piwnica-Worms
authors.forename: Barry P
authors.initials: BP
authors.lastname: Sleckman
authors.forename: Herbert W
authors.initials: HW
authors.lastname: Virgin
mesh_terms: D000818:Animals; D004249:DNA Damage; D018142:Gammaherpesvirinae / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D006566:Herpesviridae Infections / Q000503:physiopathology; D004854:Herpesvirus 4, Human / Q000502:physiology; D006657:Histones / Q000378:metabolism*; D006801:Humans; D016366:Open Reading Frames; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D010957:Plasmids; D011494:Protein Kinases / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication
chemical_list: C468783:H2AX protein, human; D006657:Histones; D010750:Phosphoproteins; D014764:Viral Proteins; D011494:Protein Kinases