DNA damage during glycation of lysine by methylglyoxal: assessment of vitamins in preventing damage.
Amino acids react with methylglyoxal to form advanced glycation end products. This reaction is known to produce free radicals. In this study, cleavage to plasmid DNA was induced by the glycation of lysine with methylglyoxal in the presence of iron(III). This system was found to produce superoxide as well as hydroxyl radicals. The abilities of various vitamins to prevent damage to plasmid DNA were evaluated. Pyridoxal-5-phosphate showed maximum protection, while pyridoxamine showed no protection. The protective abilities could be directly correlated to inhibition of production of hydroxyl and superoxide radicals. Pyridoxal-5-phosphate exhibited low radical scavenging ability as evaluated by its TEAC, but showed maximum protection probably by interfering in free radical production. Pyridoxamine did not inhibit free radical production. Thiamine and thiamine pyrophosphate, both showed protective effects albeit to different extents. Tetrahydrofolic acid showed better antioxidant activity than folic acid but was found to damage DNA by itself probably by superoxide generation.
Suji
G
G
Department of Life Sciences, University of Mumbai, Santacruz (E), Mumbai, India.
Sivakami
S
S
D000596:Amino Acids / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects*; D016166:Free Radical Scavengers / Q000494:pharmacology; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006031:Glycosylation; D007501:Iron / Q000378:metabolism; D008239:Lysine / Q000378:metabolism*; D010957:Plasmids; D011732:Pyridoxal Phosphate / Q000494:pharmacology; D011765:Pyruvaldehyde / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D014815:Vitamins / Q000494:pharmacology*
D000596:Amino Acids; D016166:Free Radical Scavengers; D017127:Glycation End Products, Advanced; D014815:Vitamins; D013481:Superoxides; D011732:Pyridoxal Phosphate; D011765:Pyruvaldehyde; D007501:Iron; D008239:Lysine