title: Vitreous levels of stromal cell-derived factor 1 and vascular endothelial growth factor in patients with retinopathy of prematurity.
abstract: OBJECTIVE
  To understand better the role of vascular endothelial growth factor (VEGF) and stromal cell-derived factor 1alpha (SDF-1alpha) in the pathogenesis of retinopathy of prematurity (ROP).
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  The study group consisted of 22 eyes with stage 4 ROP (12 vascularly active, 10 vascularly inactive), from which vitreous samples were obtained. Vitreous samples from 5 eyes undergoing surgery for congenital cataract were used as controls.
  
  
  METHODS
  The vitreous samples were analyzed for concentrations of total protein, VEGF, and SDF-1alpha. Vascular activity was graded at the time of surgery and was defined as the presence of plus disease, neovascularization growing onto the vitreous at the ridge or tractional detachment area, or combined effusive and tractional retinal detachment.
  
  
  METHODS
  Vitreous concentrations of VEGF and SDF-1alpha were compared among vascularly active stage 4 ROP eyes, vascularly inactive stage 4 ROP eyes, and control eyes.
  
  
  RESULTS
  Vitreous concentrations of VEGF and SDF-1alpha were elevated in eyes with ROP compared with control eyes. The median VEGF level was 59 pg/ml (range, 38-135 pg/ml) in the control group, 316 pg/ml (range, 105-665 pg/ml) in the vascularly inactive ROP group, and 3454 pg/ml (range, 774-8882 pg/ml) in the vascularly active ROP group. The median SDF-1alpha level was 327 pg/ml (range, 299-393 pg/ml) in the control group, 609 pg/ml (range, 515-1116 pg/ml) in the vascularly inactive ROP group, and 1029 pg/ml (range, 807-3015 pg/ml) in the vascularly active ROP group. The differences in both vitreous VEGF and SDF-1alpha concentrations between the three groups were statistically significant (P<0.001 and P<0.001, respectively). The eyes graded as vascularly active at the time of surgery demonstrated the highest vitreous levels of both VEGF and SDF-1alpha, and these levels were statistically significant when compared with vascularly inactive eyes (P<0.001 and P = 0.001, respectively) and control eyes (P = 0.001 and P = 0.001, respectively).
  
  
  CONCLUSIONS
  These findings confirm the increase in vitreous VEGF and SDF-1alpha levels in eyes with vascularly active stage 4 ROP. Anti-VEGF treatment may be of benefit in some eyes that develop ROP.
authors.affiliation: Associated Retinal Consultants, William Beaumont Hospital, Royal Oak, Michigan 48073, USA.
authors.forename: Kenan
authors.initials: K
authors.lastname: Sonmez
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Drenser
authors.forename: Antonio
authors.initials: A
authors.lastname: Capone
authors.forename: Michael T
authors.initials: MT
authors.lastname: Trese
mesh_terms: D002386:Cataract / Q000151:congenital; D054377:Chemokine CXCL12 / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005865:Gestational Age; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D011446:Prospective Studies; D012178:Retinopathy of Prematurity / Q000145:classification / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D014822:Vitreous Body / Q000378:metabolism*
chemical_list: D054377:Chemokine CXCL12; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A