Gene expression profile in monocyte during in vitro mineral fiber degradation.
A human monocytes cell line, U-937, incubated in the presence of filtered medium from Escherichia coli culture (FS) has been previously reported to degrade man made mineral fiber and it has been indicated as a good paradigm of in vivo fiber biopersistence evaluation (manuscript accepted for publication). In the present paper, a study is reported aimed to define the molecular modification occurring in the U-937 monocytes during in vitro fiber degradation. The induction of gene expression was investigated in U-937 exposed to rock wool fibers (HDN) in the presence of FS by transcriptome analysis using 20 K DNA microarrays and quantitative RT-PCR. The over-expression of genes related to mobility and cellular adhesion, oxidative stress, immune system stimulation, enzymes, and ions transport protein systems were identified. Among them NCF1 gene, the gene encoding a subunit of NADPH oxidase, over-expression was detected. As the product of this gene allows the formation of superoxide anion that could lead to oxidative stress, HDN fibers were exposed to hydrogen peroxide. Fiber degradation similar to those observed upon incubation with U-937 in the presence of FS was obtained thus suggesting that reactive oxygen species production may be responsible for fiber degradation by U-937 monocytes.
Dika Nguea
Hermine
H
Laboratoire de Bactériologie Virologie, Faculté de Médecine, Nancy-University, 9 Avenue de la Forêt de Haye BP 184, 54505, Vandoeuvre-lès-Nancy Cedex, France.
de Reydellet
Aymon
A
Lehuédé
Patrice
P
De Meringo
Alain
A
Le Faou
Alain
A
Marcocci
Lucia
L
Rihn
Bertrand H
BH
D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology*; D004359:Drug Therapy, Combination; D004926:Escherichia coli / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D019016:Mineral Fibers / Q000633:toxicity*; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D020298:U937 Cells
D017077:Culture Media, Conditioned; D019016:Mineral Fibers; D017382:Reactive Oxygen Species; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1