Improved determination of 5-fluorouracil and its prodrug tegafur in pharmaceuticals by large-volume sample stacking in CE.
On-line determination of the anti-tumor drug 5-fluorouracil (5-FU) and its prodrug, tegafur (TF) was achieved for the first time by capillary electrophoresis with large-volume sample stacking (CE-LVSS). The optimal electrophoretic buffer consisted of 30 mM phosphate buffer at pH 8.0. Without the LVSS procedure, the limits of detection (LOD) were 600.5 ng/mL and 771.4 ng/mL for 5-FU and TF, respectively. With the LVSS procedure, the sensitivity was significantly improved by about two orders of magnitude (the LODs of 5-FU and TF were decreased to 7.9 ng/mL and 6.5 ng/mL, respectively). The %RSD was less than 5%. This method compared favorably with other reported techniques and was applied successfully to the quantitative analysis of anti-tumor drugs in commercial injection preparations. The results show that the method is simple, fast (less than 3 min), highly selective, and sensitive.
Yang
Yanmin
Y
College of Chemistry & Chemical Engineering, Hunan University, Changsha, China.
Liu
Qian
Q
Tao
Wenyan
W
Nie
Lihua
L
Yao
Shouzhuo
S
D019075:Electrophoresis, Capillary / Q000379:methods*; D005472:Fluorouracil / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D004364:Pharmaceutical Preparations; D011355:Prodrugs / Q000032:analysis* / Q000737:chemistry; D005641:Tegafur / Q000032:analysis* / Q000737:chemistry
D004364:Pharmaceutical Preparations; D011355:Prodrugs; D005641:Tegafur; D005472:Fluorouracil