Clonal characterization of sporadic cribriform-morular variant of papillary thyroid carcinoma by laser microdissection-based APC mutation analysis.
Cribriform-morular variant (C-MV) of papillary thyroid carcinoma (PTC) is a rare and unusual neoplasm composed of multiple histologic components, including cribriform, papillary, solid, tall columnar, and morular patterns. Analyses of gross C-MV of PTC lesions has linked adenomatous polyposis coli (APC) mutations to its pathogenesis; however, the extent of involvement of mutations in the development of individual components is unclear. We report on bidirectional sequencing of the mutation cluster region (codons 1032-1565) of the APC gene in individually laser-microdissected components of a previously unreported C-MV of PTC. A silent Thr1493Thr gene variant was found in all tumoral components, whereas a 5-base-pair frameshift deletion at codon 1309 was identified only in the morules. Neither variant was observed in matched normal thyroid tissue. These results show the histologic components of C-MV of PTC to have some common mutational background, although additional somatic mutations may be involved in the development of morular structures.
Subramaniam
Manish Mani
MM
Oncology Research Institute, Centre for Life Sciences, National University of Singapore, Singapore.
Putti
Thomas Choudary
TC
Anuar
Diyanah
D
Chong
Pei Yi
PY
Shah
Nilesh
N
Salto-Tellez
Manuel
M
Soong
Richie
R
D000231:Adenocarcinoma, Papillary / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D000328:Adult; D001483:Base Sequence; D002999:Clone Cells; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D017491:Genes, APC; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D008969:Molecular Sequence Data; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery
D004273:DNA, Neoplasm