title: Size-exclusion chromatography based on silica-diol for the analysis of the proinsulin fusion protein.
abstract: Size-exclusion chromatography based on silica-diol sorbent was employed to analyze the recombinant proinsulin fusion protein obtained during the process of refolding and the following ion-exchange purification. The assay was qualified as a control method estimating its accuracy, precision, linearity, limit of detection, limits of quantitation, specificity, and robustness. The results show the reliability for the intended use.
authors.affiliation: Experimental Biotechnological Plant, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of Russian Academy of Sciences, Mikloukho-Maklaya Street, 16/10, Moscow 117997, Russian Federation. gusarovavd@list.ru
authors.forename: V
authors.initials: V
authors.lastname: Gusarova
authors.forename: T
authors.initials: T
authors.lastname: Vorobjeva
authors.forename: D
authors.initials: D
authors.lastname: Gusarov
authors.forename: V
authors.initials: V
authors.lastname: Lasman
authors.forename: D
authors.initials: D
authors.lastname: Bayramashvili
mesh_terms: D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D011384:Proinsulin / Q000032:analysis*; D011993:Recombinant Fusion Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D011993:Recombinant Fusion Proteins; D011384:Proinsulin