Coordinated action of N-CAM, N-cadherin, EphA4, and ephrinB2 translates genetic prepatterns into structure during somitogenesis in chick.
During gastrulation in vertebrates, mesenchymal cells at the anterior end of the presomitic mesoderm (PSM) periodically compact, transiently epithelialize and detach from the posterior PSM to form somites. In the prevailing clock-and-wavefront model of somitogenesis, periodic gene expression, particularly of Notch and Wnt, interacts with an FGF8-based thresholding mechanism to determine cell fates. However, this model does not explain how cell determination and subsequent differentiation translates into somite morphology. In this paper, we use computer simulations of chick somitogenesis to show that experimentally-observed temporal and spatial patterns of adhesive N-CAM and N-cadherin and repulsive EphA4-ephrinB2 pairs suffice to reproduce the complex dynamic morphological changes of somitogenesis in wild-type and N-cadherin (-/-) chick, including intersomitic separation, boundary-shape evolution and sorting of misdifferentiated cells across compartment boundaries. Since different models of determination yield the same, experimentally-observed, distribution of adhesion and repulsion molecules, the patterning is independent of the details of this mechanism.
Glazier
James A
JA
Biocomplexity Institute and Department of Physics, 727 East Third Street, Indiana University, Bloomington, Indiana 47405, USA.
Zhang
Ying
Y
Swat
Maciej
M
Zaitlen
Benjamin
B
Schnell
Santiago
S
D000818:Animals; D019521:Body Patterning / Q000235:genetics; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion; D002642:Chick Embryo / Q000196:embryology* / Q000378:metabolism; D003198:Computer Simulation; D036388:Ephrin-B2 / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D008954:Models, Biological; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism; D036122:Receptor, EphA4 / Q000378:metabolism; D019170:Somites / Q000196:embryology* / Q000378:metabolism
D015820:Cadherins; D036388:Ephrin-B2; D019006:Neural Cell Adhesion Molecules; D036122:Receptor, EphA4