A facile method for the detection of RNA-polypeptide interactions by MALDI-TOF mass spectrometry.
The development of soft ionization methods for mass spectrometry has made possible the analysis of large and relatively unstable biological samples. However, the analysis of RNA-polypeptide interactions has been hampered by the low detection sensitivity to RNA and the resulting RNA-polypeptide complexes. In this study, we attempted the detection of RNA-polypeptide complexes by MALDI-TOF MS, focusing on the disappearance of the peptide signal upon binding to the RNA. It was demonstrated that specifically RNA-binding peptides could be identified from a mixture of peptides, possibly enabling high throughput screening of RNA-binding polypeptides.
Sugaya
Maki
M
Department of Materials and Life Science, Faculty of Science and Technology, Seikei University, Musashino, Tokyo 180-8633, Japan.
Harada
Kazuo
K
Katoh
Akira
A
D000595:Amino Acid Sequence; D001120:Arginine / Q000032:analysis; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000032:analysis / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D012367:RNA, Viral / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010455:Peptides; D012367:RNA, Viral; D012313:RNA; D001120:Arginine