Visual snapshots of intracellular kinase activity at the onset of mitosis.
Visual snapshots of intracellular kinase activity can be acquired with exquisite temporal control by using a light-activatable (caged) sensor, thereby providing a means to interrogate enzymatic activity at any point during the cell-division cycle. Robust protein kinase activity transpires just prior to, but not immediately after, nuclear envelope breakdown (NEB). Furthermore, kinase activity is required for the progression from prophase into metaphase. Finally, the application of selective protein kinase C (PKC) inhibitors, in combination with the caged sensor, correlates the action of the PKC beta isoform with subsequent NEB.
Dai
Zhaohua
Z
Department of Biochemistry, The Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
Dulyaninova
Natalya G
NG
Kumar
Sanjai
S
Bresnick
Anne R
AR
Lawrence
David S
DS
D002460:Cell Line; D008938:Mitosis; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D047428:Protein Kinase Inhibitors; D011494:Protein Kinases