title: Mutational analysis on the S-domain of bacterial ribonuclease P ribozyme.
abstract: We have prepared a temporal engineering template for Escherichia coli RNase P RNA that has additional restriction sites on the S-domain region. We have prepared several deletion variants of S-domain region. The results showed the P12 and P13 domains are necessary for efficient enzymatic reactions and the absence of P14 and P17 domains are easily rescued by the presence of high concentration of magnesium ions.
authors.affiliation: Division of Bioscience and Biotechnology, Department of Ecological Engineering, Toyohashi Universitry of Technology, Tempakucho, Toyohashi, Aichi 441-8580, Japan.
authors.forename: Terumichi
authors.initials: T
authors.lastname: Tanaka
authors.forename: Yo
authors.initials: Y
authors.lastname: Kikuchi
mesh_terms: D001483:Base Sequence; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005818:Genetic Engineering; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D043262:Ribonuclease P / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D043262:Ribonuclease P; C470865:ribonuclease P, E coli