Photochemical synthesis of R-shaped DNA via 5-cyanovinyldeoxyuridine.
We now report template-directed photoligation using ODN containing alpha(C)U and beta(C)U at both terminal ends. By using this method, we performed template-directed photoligation in two positions at the middle of DNA and synthesized R-shaped DNA as novel structure. The isolated R-shaped DNA was characterized by MALDI-TOF MS and enzymatic digestion.
Ogino
Masayuki
M
The School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
Fujimoto
Kenzo
K
D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000528:radiation effects; D010777:Photochemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013698:Templates, Genetic
C509569:5-cyanovinyldeoxyuridine; D009838:Oligodeoxyribonucleotides; D004247:DNA; D003857:Deoxyuridine