title: Significance of urinary excretion of 8-hydroxy-2'-deoxyguanosine in healthy subjects and liver disease patients.
abstract: OBJECTIVE
  Although the importance of reactive oxygen species (ROS) in the pathogenesis of various diseases is stressed, clinical significance of the markers reflecting DNA oxidation such as 8-hydroxy-2'-deoxyguanosine (8-OHdG) remains to be clarified.
  
  
  METHODS
  To examine clinical usefulness of 8-OHdG in healthy individuals in comparison with liver disease patients, urinary excretion of 8-OHdG was measured in 336 healthy individuals and 110 patients with liver disease.
  
  
  RESULTS
  In healthy persons, the 8-OHdG excretion was increased in an age-dependent manner. It was positively correlated with cigarettes smoked a day and negatively correlated with body mass index (BMI) (P < 0.05, each). Age, smoking and BMI were independent predictors of urinary 8-OHdG excretion (P < 0.01, P < 0.01 and P < 0.05, respectively). In liver disease, the excretion of 8-OHdG was not changed, as compared with healthy individuals. However, the liver disease patients under the age of 40 had higher values of 8-OHdG than healthy persons. In addition, the urinary excretion of 8-OHdG was higher in patients with hepatitis C virus (HCV) infection than those with hepatitis B virus (HBV) infection.
  
  
  CONCLUSIONS
  The results of the present study suggest that measurement of urinary 8-OHdG excretion is useful in assessing DNA oxidation caused by aging, smoking, body composition and liver disease.
authors.affiliation: Division of Molecular and Genetic Medicine, Department of Gene and Regenerative Medicine, Graduate School of Medicine, Tottori University, Yonago 683-8504, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Ichiba
authors.forename: Sadako
authors.initials: S
authors.lastname: Yamada
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ishii
authors.forename: Kazue
authors.initials: K
authors.lastname: Gonda
authors.forename: Rie
authors.initials: R
authors.lastname: Murai
authors.forename: Takashi
authors.initials: T
authors.lastname: Shimomura
authors.forename: Toshiya
authors.initials: T
authors.lastname: Saeki
authors.forename: Takamasa
authors.initials: T
authors.lastname: Kanbe
authors.forename: Yoshitada
authors.initials: Y
authors.lastname: Tanabe
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsuchiya
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Hoshikawa
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kurimasa
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Kishimoto
authors.forename: Hironaka
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Goshi
authors.initials: G
authors.lastname: Shiota
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D015992:Body Mass Index; D004247:DNA / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000652:urine*; D008297:Male; D008875:Middle Aged; D010100:Oxygen / Q000378:metabolism; D017382:Reactive Oxygen Species; D012016:Reference Values; D015203:Reproducibility of Results; D012907:Smoking
chemical_list: D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine; D010100:Oxygen