[Construction of tissue engineered gingiva with gingival epithelia and acellular dermal matrix].
OBJECTIVE
To culture human gingival epithelia in vitro, and to construct the tissue engineered gingiva with the acellular dermal matrix (ADM).


METHODS
Human gingival epithelia were isolated from the gingival tissue, and the cells were cultured and seeded onto the surface of ADM. After 7 days of submerged incubation, an air-liquid interface culture was performed for 7, 14, and 21 days. The complex constructed above was taken for histological examination.


RESULTS
Human gingival epithelia could proliferate well on the surface of ADM, and form multilayer structure. But the superficial epithelium was partially keratinized.


CONCLUSIONS
Tissue engineered gingiva may be constructed with human gingival epithelia and ADM in vitro.
Fang
Chang-yun
CY
Department of Stomatology, Xiangya Hospital, Changsha 410008, China. cyfangdental@mail.csu.edu.cn.
Xie
Xiao-li
XL
Liu
Bin-jie
BJ
Su
Zheng
Z
Li
Hui-li
HL
Shi
Xiao-ming
XM
Li
Ming
M
Pang
Na
N
Zhong
Wei-ying
WY
D062088:Acellular Dermis; D002478:Cells, Cultured; D003238:Connective Tissue; D004847:Epithelial Cells / Q000166:cytology*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods*