title: An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose.
abstract: An ethanol-tolerant mutant, ET1, was isolated by an enrichment method from Escherichia coli JM109. Strains JM109 and ET1 were transformed with expression vector pZY507bc containing Zymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes, resulting in an ethanol-sensitive recombinant strain JMbc and an ethanol-tolerant recombinant strain, ET1bc. Alcohol dehydrogenase and pyruvate decarboxylase activities were 24 and 32% lower, respectively, in JMbc than in ET1bc. ET1bc fermented 10% (w/v) xylose to give 39.4 g ethanol/l (77%, theoretical yield), a 1.3-fold increase compared with the ethanol-sensitive strain JMbc.
authors.affiliation: College of Biological Sciences, China Agricultural University, Beijing 100094, P.R. China.
authors.forename: Zhanchao
authors.initials: Z
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Xu
authors.forename: Shuying
authors.initials: S
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Ping
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Lin
mesh_terms: D000222:Adaptation, Physiological; D000426:Alcohol Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D015870:Gene Expression; D011767:Pyruvate Decarboxylase / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014994:Xylose / Q000378:metabolism*; D017355:Zymomonas / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D000431:Ethanol; D014994:Xylose; D000426:Alcohol Dehydrogenase; D011767:Pyruvate Decarboxylase