Specific reactivity of mild/severe Alzheimer's disease patient's sera to antibody against Abeta1-40 epitope 17-21.
OBJECTIVE
To detect the reactivity pattern of sera from patients with mild and severe Alzheimer's disease (AD) to specific antibodies targeting different epitopes in the primary structure of amyloid-beta (Abeta).


METHODS
Sera from patients diagnosed with mild or severe AD were used. The reactivity of sera to monoclonal antibodies recognizing 1-7, 5-10, 9-14 and 17-21 epitopes of Abeta1-40 at 36-42 degrees C was determined by an enzyme-linked immunosorbent assay. Proteinase K digestion of Abeta1-40 was investigated by dot blotting at 36 and 40 degrees C.


RESULTS
Sera of patients with AD displayed reactivity only with monoclonal antibody recognizing the epitope 17-21 (4G8). The reactivity of sera from patients with severe AD was less than that of sera from patients with mild AD at temperatures 36-41 degrees C, with no difference at 42 degrees C. Patients with severe AD displayed lesser digestion with proteinase K.


CONCLUSIONS
Sera derived from patients with AD could react with monoclonal antibodies directed to 17-21 sequences of Abeta1-40 in a temperature-dependent manner. The severity of AD is associated with greater Abeta1-40 aggregation and resistance to proteinase K. The present results may be of value in staging and following up of patients with AD.
Bolukbasi Hatip
F F
FF
Department of Pharmacology, Faculty of Medicine, Pamukkale University, Kinikli Campus, Denizli, Turkey.
Matsunaga
Y
Y
Yamada
T
T
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000276:immunology*; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D019286:Endopeptidase K / Q000737:chemistry; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology*
D016229:Amyloid beta-Peptides; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007106:Immune Sera; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); D019286:Endopeptidase K