title: Rotavirus infection induces the phosphorylation of eIF2alpha but prevents the formation of stress granules.
abstract: Early during the infection process, rotavirus causes the shutoff of cell protein synthesis, with the nonstructural viral protein NSP3 playing a vital role in the phenomenon. In this work, we have found that the translation initiation factor 2alpha (eIF2alpha) in infected cells becomes phosphorylated early after virus infection and remains in this state throughout the virus replication cycle, leading to a further inhibition of cell protein synthesis. Under these restrictive conditions, however, the viral proteins and some cellular proteins are efficiently translated. The phosphorylation of eIF2alpha was shown to depend on the synthesis of three viral proteins, VP2, NSP2, and NSP5, since in cells in which the expression of any of these three proteins was knocked down by RNA interference, the translation factor was not phosphorylated. The modification of this factor is, however, not needed for the replication of the virus, since mutant cells that produce a nonphosphorylatable eIF2alpha sustained virus replication as efficiently as wild-type cells. In uninfected cells, the phosphorylation of eIF2alpha induces the formation of stress granules, aggregates of stalled translation complexes that prevent the translation of mRNAs. In rotavirus-infected cells, even though eIF2alpha is phosphorylated these granules are not formed, suggesting that the virus prevents the assembly of these structures to allow the translation of its mRNAs. Under these conditions, some of the cellular proteins that form part of these structures were found to change their intracellular localization, with some of them having dramatic changes, like the poly(A) binding protein, which relocates from the cytoplasm to the nucleus in infected cells, a relocation that depends on the viral protein NSP3.
authors.affiliation: Instituto de Biotecnología, UNAM, Avenida Universidad 2001, Colonia Chamilpa, Cuernavaca, Morelos 62210, Mexico.
authors.forename: Hilda
authors.initials: H
authors.lastname: Montero
authors.forename: Margarito
authors.initials: M
authors.lastname: Rojas
authors.forename: Carlos F
authors.initials: CF
authors.lastname: Arias
authors.forename: Susana
authors.initials: S
authors.lastname: López
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000502:physiology; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000378:metabolism*; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism*; D020868:Gene Silencing; D008253:Macaca mulatta; D051379:Mice; D010766:Phosphorylation; D039102:Poly(A)-Binding Proteins / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000502:physiology; D012401:Rotavirus / Q000502:physiology*; D017361:Viral Nonstructural Proteins / Q000502:physiology
chemical_list: D036022:Capsid Proteins; D015852:Eukaryotic Initiation Factor-2; C082740:NSP3 protein, Rotavirus; D039102:Poly(A)-Binding Proteins; D016601:RNA-Binding Proteins; C442545:VP2 protein, Rotavirus; D017361:Viral Nonstructural Proteins; C079234:NS35 protein, rotavirus