title: Canine cancer screening via ultraviolet absorbance and fluorescence spectroscopy of serum proteins.
abstract: A cost-effective optical cancer screening and monitoring technique was demonstrated in a pilot study of canine serum samples and was patented for commercialization. Compared to conventional blood chemistry analysis methods, more accurate estimations of the concentrations of albumin, globulins, and hemoglobin in serum were obtained by fitting the near UV absorbance and photoluminescence spectra of diluted serum as a linear combination of component reference spectra. Tracking these serum proteins over the course of treatment helped to monitor patient immune response to carcinoma and therapy. For cancer screening, 70% of dogs with clinical presentation of cancer displayed suppressed serum hemoglobin levels (below 20 mg/dL) in combination with atypical serum protein compositions, that is, albumin levels outside of a safe range (from 4 to 8 g/dL) and globulin levels above or below a more normal range (from 1.7 to 3.7 g/dL). Of the dogs that met these criteria, only 20% were given a false positive label by this cancer screening test.
authors.affiliation: Luna Innovations Incorporated, 3157 State Street, Blacksburg, Virginia 24060, USA.
authors.forename: Bryan D
authors.initials: BD
authors.lastname: Dickerson
authors.forename: Brian L
authors.initials: BL
authors.lastname: Geist
authors.forename: William B
authors.initials: WB
authors.lastname: Spillman
authors.forename: John L
authors.initials: JL
authors.lastname: Robertson
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods; D001798:Blood Proteins / Q000032:analysis*; D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D004285:Dogs; D009363:Neoplasm Proteins / Q000097:blood*; D009369:Neoplasms / Q000097:blood* / Q000175:diagnosis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins; D009363:Neoplasm Proteins