Development of a specific fluorescent probe for 8-oxoguanosine.
8-Oxoguanosine (8-oxoG) is derived from the oxidation of guanosine (dG) and is known to induce transversion mutations (G:C to T:A) in DNA. We have already reported the fluorescent probe "8-oxoG-clamp", which shows a selective fluorescence quenching phenomenon toward 8-oxoG. In this study, attachment of an additional fluorophore to 8-oxoG-clamp was investigated as a strategy for the detection of 8-oxoG by a fluorescence color change attributed to the 8-oxoG-clamp.
Ono
Sayaka
S
Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Li
Zhichun
Z
Koga
Yohei
Y
Tsujimoto
Akira
A
Nakagawa
Osamu
O
Sasaki
Shigeki
S
D020029:Base Pairing; D002219:Carbamates / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006151:Guanosine / Q000031:analogs & derivatives* / Q000032:analysis; D011744:Pyrimidinones / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D002219:Carbamates; C522074:Cbz-8-oxoG-clamp; D005456:Fluorescent Dyes; D011744:Pyrimidinones; D006151:Guanosine; C046215:8-hydroxyguanosine