title: Monitoring of Eco RI-catalyzed cleavage reaction of fluorescent-labeled heterochiral DNA.
abstract: We have found the unusual reactivity of a heterochiral oligodeoxynucleotide toward restriction endonuclease Eco RI. To conduct the kinetic analysis of the reaction, fluorescent-labeled single-stranded oligodeoxynucleotide molecular beacons were designed and synthesized. The beacons showed a remarkable fluorescence response by addition of Eco RI. The results promise that the beacon could be an effective tool for the kinetic analysis of Eco RI-catalyzed cleavage reaction of the heterochiral oligodeoxynucleotide.
authors.affiliation: Osaka university of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. urata@gly.oups.ac.jp
authors.forename: Hidehito
authors.initials: H
authors.lastname: Urata
authors.forename: Chihiro
authors.initials: C
authors.lastname: Tamaki
authors.forename: Miki
authors.initials: M
authors.lastname: Matsuno
authors.forename: Shun-Ichi
authors.initials: S
authors.lastname: Wada
authors.forename: Masao
authors.initials: M
authors.lastname: Akagi
mesh_terms: D002384:Catalysis; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI / Q000032:analysis* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D004247:DNA; D015246:Deoxyribonuclease EcoRI