title: Determination of phenylalanine tRNA recognition sites by phenylalanyl-tRNA synthetase from hyperthermophilic archaeon, Aeropyrum pernix K1.
abstract: Phenylalanine tRNA identity has been determined in the bacteria and the eukaryote system, but remains unknown for the archaea system. To investigate the molecular recognition mechanism of phenylalanine tRNA by phenylalanyl-tRNA synthetase from hyperthermophilic and aerobic archaeon, Aeropyrum pernix K1, various mutant transcripts of phenylalanine tRNA prepared by an in vitro transcription system were examined by overexpressed A. pernix phenylalanyl tRNA synthetase. The results indicated that anticodon nucleotides G34, A35 and A36, discriminator base A73 and G20 in the variable pocket were base-specifically recognized by A. pernix phenylalanyl-tRNA synthetase.
authors.affiliation: Graduate School of Science and Technology, Yamagata University, Yamagata 990-8560, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Tsuchiya
authors.forename: Manami
authors.initials: M
authors.lastname: Kimura
authors.forename: Tsunemi
authors.initials: T
authors.lastname: Hasegawa
mesh_terms: D041522:Aeropyrum / Q000201:enzymology*; D000926:Anticodon / Q000737:chemistry; D019843:Archaeal Proteins / Q000378:metabolism*; D010652:Phenylalanine-tRNA Ligase / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000737:chemistry* / Q000378:metabolism; D046249:Transfer RNA Aminoacylation
chemical_list: D000926:Anticodon; D019843:Archaeal Proteins; D012360:RNA, Transfer, Phe; D010652:Phenylalanine-tRNA Ligase