[HPLC fingerprint of flavonoids of Kushen Tang and its correlation to Scutellaria baicalensis and Sophora flavescens].
OBJECTIVE
To establish the HPLC fingerprint of the flavonoids of Kushen Tang, and to study its correlation to Scutellaria baicalensis and Sophora flavescens.


METHODS
The fingerprints of the flavonoids of Kushen Tang were established by HPLC under two detective wavelengths 280 nm and 365 nm. The correlation existed in the common peaks of HPLC fingerprint of Kushen Tang and the Chinese meteria medicines which composed the prescription was explored by analyzing the HPLC fingerprints of different compatibilities. The chemical constituents which the common peaks stood for were analyzed by adding controls to the samples respectively.


RESULTS
Under the detective wavelength 280 nm, 19 common peaks were found, which came from S. baicalensis except that two peaks came from S. flavescens, and eight peaks were baicalin/trifolirhizin, wogonoside, luteolin, baicalein, oroxylin A, 5, 8-dihydroxy-6, 7-dimethoxyflavone, wogonin and formononetin/chrysin respectively. Under the detective wavelength 365 nm, 22 common peaks were found, which thirteen peaks came from S. baicalensis and six peaks came from S. flavescens. Among them, eleven peaks were baicalin, wogonoside, baicalein, oroxylin A, 5,8-dihydroxy-6, 7-dimethoxyflavone, wogonin, chrysin/7-methoxyflavone, 2', 4-dihydroxy-4', 6'-dimethoxychalcone, 4, 4'-dimethoxychalcone, xanthohumol and kuraridin respectively.


CONCLUSIONS
The common peaks of HPLC fingerprint of the flavonoids of Kushen Tang, which were obtained under the two detective wavelengths 280 nm and 365 nm, came from S. baicalensis and S. flavescens. It was more scientific and comprehensive that establishing the HPLC fingerprint of the flavonoids of Kushen Tang under the detective wavelength 365 nm.
Liu
Bin
B
School of Chinese Pharmacology, Beijing University of Chinese Medicine, Beijing 100102, China. liubinyn67@163.com
Shi
Ren-bing
RB
Zhu
Li-jun
LJ
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D044950:Flavanones / Q000032:analysis; D005419:Flavonoids / Q000032:analysis*; D005960:Glucosides / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D032267:Rehmannia / Q000737:chemistry; D031364:Scutellaria baicalensis / Q000737:chemistry*; D029909:Sophora / Q000737:chemistry*
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; C532369:kushen tang; C006680:baicalein; C473995:wogonoside; C085514:wogonin