Angiostrongylus cantonensis: induction of urokinase-type PA and degradation of type IV collagen in rat lung granulomatous fibrosis.
Granuloma formation and subsequent fibrosis around Angiostrongylus cantonensis larvae in the lungs were induced experimentally in Sprague-Dawley strain rats. Casein zymogram analysis demonstrated that urokinase-type plasminogen activator (uPA) activity was increased during lung inflammation and fibrosis. Granulomatous fibrosis, type IV collagen degradation and activation of uPA occur simultaneously. Furthermore, the present study demonstrated that collagen avidly binds uPA. Immunohistochemical observations showed localization of uPA within the infiltrating leucocytes. We propose that uPA may participate in A. cantonensis-induced granulomatous fibrosis.
Lan
Kuang-Ping
KP
Department of Laboratory, CiShan Hospital, CiShan Chen, Kaohsiung County 842, Taiwan.
Lai
Shih-Chan
SC
D017185:Angiostrongylus cantonensis / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D002364:Caseins / Q000378:metabolism; D024141:Collagen Type IV / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D006099:Granuloma / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008168:Lung / Q000201:enzymology / Q000469:parasitology / Q000473:pathology; D008297:Male; D011658:Pulmonary Fibrosis / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013047:Specific Pathogen-Free Organisms; D017206:Strongylida Infections / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis*
D002364:Caseins; D024141:Collagen Type IV; D014568:Urokinase-Type Plasminogen Activator