Cyclin-dependent kinase 5 is required for control of neuroblast migration in the postnatal subventricular zone.
At the lateral wall of the lateral ventricles in the adult rodent brain, neuroblasts form an extensive network of elongated cell aggregates called chains in the subventricular zone and migrate toward the olfactory bulb. The molecular mechanisms regulating this migration of neuroblasts are essentially unknown. Here, we report a novel role for cyclin-dependent kinase 5 (Cdk5), a neuronal protein kinase, in this process. Using in vitro and in vivo conditional knock-out experiments, we found that Cdk5 deletion impaired the chain formation, speed, directionality, and leading process extension of the neuroblasts in a cell-autonomous manner. These findings suggest that Cdk5 plays an important role in neuroblast migration in the postnatal subventricular zone.
Hirota
Yuki
Y
Department of Developmental and Regenerative Biology, Institute of Molecular Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan.
Ohshima
Toshio
T
Kaneko
Naoko
N
Ikeda
Makiko
M
Iwasato
Takuji
T
Kulkarni
Ashok B
AB
Mikoshiba
Katsuhiko
K
Okano
Hideyuki
H
Sawamoto
Kazunobu
K
D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D018920:Coculture Techniques; D051360:Cyclin-Dependent Kinase 5 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D006801:Humans; D020547:Lateral Ventricles / Q000166:cytology / Q000201:enzymology / Q000254:growth & development*; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D009474:Neurons / Q000166:cytology / Q000201:enzymology*; D013234:Stem Cells / Q000201:enzymology / Q000502:physiology*
D051360:Cyclin-Dependent Kinase 5