title: Recognition of longer duplex DNA by cooperative strand invasion.
abstract: Peptide nucleic acid is a synthetic DNA mimic in which the sugar-phosphate backbone has been replaced by a peptide backbone. A remarkable feature of PNA is its ability to recognize sequences within duplex DNA by strand invasion. We have previously demonstrated that a PNA targeting six bases within duplex DNA cooperatively binds to 12 base-pair site by strand invasion. We here report an successful extension of the target site size to 18 base pairs without the expense of specificity.
authors.affiliation: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan. csugi@mail.ecc.u-tokyo.ac.jp
authors.forename: Toru
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Yasutada
authors.initials: Y
authors.lastname: Imamura
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kurihara
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kittaka
mesh_terms: D020137:Base Pair Mismatch; D004247:DNA / Q000737:chemistry*; D024202:Electrophoretic Mobility Shift Assay; D020135:Peptide Nucleic Acids / Q000737:chemistry*
chemical_list: D020135:Peptide Nucleic Acids; C051802:triplex DNA; D004247:DNA