title: Connective tissue progenitor cell growth characteristics on textured substrates.
abstract: Growth characteristics of human connective tissue progenitor (CTP) cells were investigated on smooth and textured substrates, which were produced using MEMS (microelectromechanical systems) fabrication technology. Human bone marrow derived cells were cultured for 9 days under conditions promoting osteoblastic differentiation on polydimethylsiloxane (PDMS) substrates comprising smooth (non-patterned) surfaces (SMOOTH), 4 different cylindrical post micro-textures (POSTS) that were 7-10 microm high and 5, 10, 20, and 40 microm diameter, respectively, and channel micro-textures (CHANNELS) with curved cross-sections that were 11 microm high, 45 microm wide, and separated by 5 microm wide ridges. Standard glass-tissue culture surfaces were used as controls. Micro-textures resulted in the modification of CTP morphology, attachment, migration, and proliferation characteristics. Specifically, cells on POSTS exhibited more contoured morphology with closely packed cytoskeletal actin microfilaments compared to the more random orientation in cells grown on SMOOTH. CTP colonies on 10 gm-diameter POSTS exhibited higher cell number than any other POSTS, and a significant increase in cell number (442%) compared to colonies on SMOOTH (71%). On CHANNELS, colonies tended to be denser (229%) than on POSTS (up to 140% on 10 microm POSTS), and significantly more so compared to those on SMOOTH (104%).
authors.affiliation: Department of Chemical and Biomedical Engineering, Cleveland State University, Ohio, USA.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Mata
authors.forename: Cynthia
authors.initials: C
authors.lastname: Boehm
authors.forename: Aaron J
authors.initials: AJ
authors.lastname: Fleischman
authors.forename: George F
authors.initials: GF
authors.lastname: Muschler
authors.forename: Shuvo
authors.initials: S
authors.lastname: Roy
mesh_terms: D000818:Animals; D018786:Bone Substitutes; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods; D009757:Nylons / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D010316:Particle Size; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D018786:Bone Substitutes; D004129:Dimethylpolysiloxanes; D009757:Nylons; C085707:poly(dimethylsiloxane)-polyamide copolymer