Quantitative expression of lysophosphatidic acid receptor 3 gene in porcine endometrium during the periimplantation period and estrous cycle.
Lysophosphatidic acid (LPA) belongs to the group of lipid messengers, which act via lysophosphatidic acid receptor 3 coupled to G-proteins. The participation of LPA3 in reproductive biology was revealed in mice and has not been studied in gilts. The present study was performed to evaluate the gene expression of LPA3 by a quantitative real-time PCR technique in the endometrium during different stages of pregnancy (days 6-30) and corresponding days of the estrous cycle (days 2-20) as well as in periimplantation period in pigs with surgically detached uterine horns. Based on the most conserved segments of human and rodent LPA3 we obtained a product containing 619bp (GenBank: EF137953), which exhibited high homology with human and rodents sequences. The highest transcript level was noted on days 10-12 of gestation in comparison to remaining periods and during pregnancy on days: 6-7, 8-9, 10-12 and 13-14 in comparison with the corresponding days of the estrous cycle. Higher mRNA level was noted in the horn containing embryos compared to the contralateral horn, where embryos did not develop. The results imply the important role of receptor LPA3 during early pregnancy.
Kamińska
Katarzyna
K
Institute of Animal Reproduction and Food Research of Polish Academy of Science, Tuwima 10, 10-747 Olsztyn, Poland.
Wasielak
Marta
M
Bogacka
Iwona
I
Blitek
Michał
M
Bogacki
Marek
M
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism*; D004971:Estrus; D005260:Female; D006801:Humans; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D049368:Receptors, Lysophosphatidic Acid / Q000737:chemistry / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D049368:Receptors, Lysophosphatidic Acid