The level of matrix metalloproteinase-2 in donor plasma strongly correlates with post-transfusion recovery of stored platelet concentrates.
OBJECTIVE
A simple test accurately predicting post-transfusion recovery of stored platelet concentrates (PCs) is needed.


METHODS
Matrix metalloproteinase-2 (MMP-2) was determined in fresh-frozen plasma of normal donors using enzyme-linked immunosorbent assay (ELISA)-based assay. Post-transfusion recovery of human PCs was determined in rabbits with an inhibited reticuloendothelial system by flow cytometry.


RESULTS
We demonstrated a strong positive (r = 0.91) and highly significant (P = 0.0015) correlation between the concentration of MMP-2 in plasma of normal donors and post-transfusion recovery of PCs stored for 5 days at 22 degrees C under standard blood banking conditions. A higher level of donor plasma MMP-2 is associated with improved recovery of transfused platelets.


CONCLUSIONS
These observations suggest that pre-transfusion MMP-2 assay of donor's plasma may be a predictor of post-transfusion recovery of PCs. However, the usefulness of this assay should be elucidated in clinical transfusion settings.
Mutlu
A
A
Division of Transfusion Medicine, Department of Laboratory Medicine, The Keenan Research Centre in the Li Ka Shing Knowledge Institute of St Michael's Hospital, Toronto, ON, Canada.
Allen
D J
DJ
Freedman
J
J
Leytin
V
V
D000818:Animals; D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000097:blood*; D010949:Plasma / Q000201:enzymology; D010976:Platelet Count; D017713:Platelet Transfusion / Q000592:standards*; D011237:Predictive Value of Tests; D011817:Rabbits
D020778:Matrix Metalloproteinase 2