title: Point mutation assay by affinity capillary electrophoresis using poly(ethylene glycol)-oligodeoxyribonucleotide block copolymers.
abstract: An affinity capillary electrophoresis was developed for detecting a point mutation of single-stranded DNA (ssDNA). Poly(ethylene glycol)-oligodeoxyribonucleo-tide block copolymers (PEG-b-ODN) were prepared to use as a novel affinity ligand. Optimization of the analytical conditions, such as the salt concentration of running buffer, the capillary temperature and the base number of the copolymer, gave two distinct peaks for wild-type (WT) and point-mutated ssDNA (MT) on the electropherogram, allowing for the facile discrimination of the single-base difference.
authors.affiliation: RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Kimura
authors.forename: Tohru
authors.initials: T
authors.lastname: Takarada
authors.forename: Naoki
authors.initials: N
authors.lastname: Kanayama
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shibata
authors.forename: Mizuo
authors.initials: M
authors.lastname: Maeda
mesh_terms: D004252:DNA Mutational Analysis / Q000379:methods*; D004277:DNA, Single-Stranded / Q000737:chemistry; D019075:Electrophoresis, Capillary; D008024:Ligands; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D017354:Point Mutation; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D004277:DNA, Single-Stranded; D008024:Ligands; D009838:Oligodeoxyribonucleotides; D011092:Polyethylene Glycols