Transcriptome analysis of NF-kappaB- and phosphatidylinositol 3-kinase-regulated genes in human cytomegalovirus-infected monocytes.
Human cytomegalovirus induces a proinflammatory monocyte following infection, and we have evidence that NF-kappaB and phosphatidylinositol 3-kinase [PI(3)K] are key mediators in this early activation. To begin to address how these signaling pathways are responsible for the rapid activation of infected monocytes, we examined the role that these pathways played in the transcriptome of infected monocytes. Global transcriptional profiling using cDNA microarrays revealed that a significant number of genes, including inflammatory genes, were regulated in an NF-kappaB- and/or PI(3)K-dependent manner, identifying the NF-kappaB and PI(3)K pathways as key cellular control points in the conversion of monocytes to an activated proinflammatory state following HCMV infection.
Chan
Gary
G
Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130-3932, USA.
Bivins-Smith
Elizabeth R
ER
Smith
M Shane
MS
Yurochko
Andrew D
AD
D002460:Cell Line; D003587:Cytomegalovirus / Q000276:immunology*; D020869:Gene Expression Profiling; D006801:Humans; D009000:Monocytes / Q000821:virology*; D016328:NF-kappa B / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology*
D016328:NF-kappa B; D019869:Phosphatidylinositol 3-Kinases