title: RNA analysis with a novel fluorescent oligonucleotide.
abstract: We have designed and synthesized a novel fluorescent oligonucleotide to analyze functions of RNAs. This probe worked as a green fluorophore only when making a stable double-strand with DNA or RNA. The fluorescence enhancement is rapid, large and target specific. Therefore, this probe has an ideal photophysical property as a fluorescent probe. We applied a fluorescent oligonucleotide to living cells to confirm the condition for introducing the probe. This novel probe and analyzing method will be a very useful tool for understanding RNA functions.
authors.affiliation: Frontier Reserarch System, RIKEN, 21 Hirosawa, Wako, Saitama 3510198, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kubota
authors.forename: Shuji
authors.initials: S
authors.lastname: Ikeda
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D015345:Oligonucleotide Probes / Q000737:chemistry*; D012313:RNA / Q000032:analysis*; D013696:Temperature
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D012313:RNA