Development and characterization of choline and L-glutamate biosensor integrated on silicon microprobes for in-vivo monitoring.
A silicon microprobe to measure in real-time choline and L-glutamate in brain tissue is described. The biosensors are prepared by coating integrated platinum thin-film electrodes with an enzymatic membrane by electrochemically aided deposition. This method allows a very local and reproducible immobilization of the enzymes and the achievement of high sensitivities as required for in vivo recordings. Moreover, several closely-spaced electrode sites on the same microprobe can be coated with different enzyme membranes without any cross-contamination.
Frey
Olivier
O
Sensors, Actuators and Microsystems Laboratory, Institute of Microtechnology, University of Neuchâtel, 2000 Neuchâtel, Switzerland. olivier.frey@unine.ch
van der Wal
Peter
P
de Rooij
Nico
N
Koudelka-Hep
Milena
M
D000429:Alcohol Oxidoreductases / Q000737:chemistry; D000594:Amino Acid Oxidoreductases / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002794:Choline / Q000032:analysis*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D018698:Glutamic Acid / Q000032:analysis*; D008839:Microelectrodes; D008904:Miniaturization; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012825:Silicon / Q000737:chemistry*; D018511:Systems Integration
D018698:Glutamic Acid; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D000594:Amino Acid Oxidoreductases; C023061:L-glutamate oxidase; D002794:Choline; D012825:Silicon