title: Thermus thermophilus tmRNA and trans-translation.
abstract: In trans-translation, ribosome switches the template for protein synthesis from an mRNA to tmRNA. The molecular mechanism of trans-translation remains mysterious. In order to clarify how tmRNA move through the ribosome, we developed in vitro systems to monitor trans-translation as well as translation, which are composed of ribosome, elongation factors, tmRNA and SmpB all from Thermus thermophilus. In these systems, the early steps of trans-translation including trans-transfer and the resume codon decoding could be monitored. Using these systems, the function of ribosomal protein S1 which has been suggested to be involved in trans-translation was investigated.
authors.affiliation: Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 0368561, Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Takada
authors.forename: Chie
authors.initials: C
authors.lastname: Takemoto
authors.forename: Masahito
authors.initials: M
authors.lastname: Kawazoe
authors.forename: Mikako
authors.initials: M
authors.lastname: Shirouzu
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Akira
authors.initials: A
authors.lastname: Muto
authors.forename: Hyouta
authors.initials: H
authors.lastname: Himeno
mesh_terms: D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*; D016963:Thermus thermophilus / Q000235:genetics* / Q000378:metabolism
chemical_list: D012329:RNA, Bacterial; D012269:Ribosomal Proteins; C015494:ribosomal protein S1; C096222:tmRNA