Expression and significance of endothelin 1 in spiral ganglion cells of guinea pig.
OBJECTIVE
Endothelin 1 has many biological activities including actions in the nervous system. This study aimed to investigate the expression of the endothelin 1 in spiral ganglion cells of guinea pig and its significance in the auditory transmission.


METHODS
Healthy guinea pigs were sacrificed and cardiac perfused with saline followed by 4% paraformaldehyde. Temporal bones were removed and fixed, decalcified in 10% EDTA, embedded in paraffin block and serially sectioned in 5 microm thick slice. Rabbit anti-endothelin 1 polyclonal antibody was used as primary antibody to examine the expression of endothelin 1 in the spiral ganglion by immunohistochemistry.


RESULTS
Endothelin 1 expression was detected in spiral ganglion cells from the basal turn to the apical turn of the cochlea.


CONCLUSIONS
The endothelin 1 presents in spiral ganglions cells of the guinea pig and might play a role in the auditory transmission.
Xu
Dingyuan
D
Department of Otorhinolaryngology, West China Hospital of Sichuan University, Chengdu 610041, China.
Tang
Yuedi
Y
Liu
Shixi
S
Liu
Jin
J
D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D019332:Endothelin-1 / Q000378:metabolism*; D006168:Guinea Pigs; D007150:Immunohistochemistry; D013136:Spiral Ganglion / Q000166:cytology* / Q000378:metabolism*
D019332:Endothelin-1