Increased individual chromosomal radiosensitivity of human lymphocytes as a parameter of cancer risk.
OBJECTIVE
Evaluation of chromosomal radiosensitivity of healthy individuals and determination those with the increased susceptibility to radiogenic cancer.


METHODS
Cytogenetic examination of radiation induced injuries in lymphocytes of healthy individuals (n=103) was carried out on the basis of G(2)-assay. Test system of peripheral blood lymphocytes with metaphase analysis was used.


RESULTS
On the basis of the obtained "stage-effect" and "dose-effect" calibrating curves the scheme of cytogenetic examinations of healthy individuals was developed. Analysis of cytogenetic parameters induced by G(2) irradiation at 1.5 Gy dose revealed their high interindividual variability. The highest differences were registered for chromatid type aberrations (CV=42.1%) with the chromatid break predominance in the spectrum (CV=37.5%). Statistical analysis of the distributions of the obtained individual cytogenetic parameters indicated 12% individuals with increased chromosomal radiosensitivity.


CONCLUSIONS
Cytogenetic evaluation of individual chromosomal radiosensitivity based on G(2)-assay has its perspectives in the formation of groups with increased risk of radiogenic cancer developing and its primary prophylactics among healthy population.
Dyomina
E A
EA
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine.
Ryabchenko
N M
NM
D002453:Cell Cycle / Q000528:radiation effects; D002478:Cells, Cultured; D002875:Chromosomes / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D009369:Neoplasms / Q000235:genetics*; D011836:Radiation Tolerance; D012307:Risk Factors