title: Analysis of molecular inversion probe performance for allele copy number determination.
abstract: We have developed a new protocol for using molecular inversion probes to accurately and specifically measure allele copy number. The new protocol provides for significant improvements, including the reduction of input DNA (from 2 mug) by more than 25-fold (to 75 ng total genomic DNA), higher overall precision resulting in one order of magnitude lower false positive rate, and greater dynamic range with accurate absolute copy number up to 60 copies.
authors.affiliation: Affymetrix Inc, Shoreline Blvd, South San Francisco, CA 94080, USA. Yuker_Wang@affymetrix.com
authors.forename: Yuker
authors.initials: Y
authors.lastname: Wang
authors.forename: Martin
authors.initials: M
authors.lastname: Moorhead
authors.forename: George
authors.initials: G
authors.lastname: Karlin-Neumann
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Wang
authors.forename: James
authors.initials: J
authors.lastname: Ireland
authors.forename: Steven
authors.initials: S
authors.lastname: Lin
authors.forename: Chunnuan
authors.initials: C
authors.lastname: Chen
authors.forename: Laura M
authors.initials: LM
authors.lastname: Heiser
authors.forename: Koei
authors.initials: K
authors.lastname: Chin
authors.forename: Laura
authors.initials: L
authors.lastname: Esserman
authors.forename: Joe W
authors.initials: JW
authors.lastname: Gray
authors.forename: Paul T
authors.initials: PT
authors.lastname: Spellman
authors.forename: Malek
authors.initials: M
authors.lastname: Faham
mesh_terms: D000483:Alleles; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D007446:Chromosome Inversion; D005189:False Positive Reactions; D006801:Humans; D019656:Loss of Heterozygosity; D015335:Molecular Probes; D016133:Polymerase Chain Reaction; D012372:ROC Curve
chemical_list: D015335:Molecular Probes