Saccharomyces cerevisiae Rad16 mediates ultraviolet-dependent histone H3 acetylation required for efficient global genome nucleotide-excision repair.
In yeast, global genome nucleotide-excision repair (GG-NER) requires a protein complex containing Rad7 and Rad16. Rad16 is a member of the switch/sucrose nonfermentable superfamily, and it is presumed that chromatin remodelling is its primary function during repair. We show that RAD16 is required for ultraviolet-dependent hyperacetylation of histone H3 (Lys 9 and Lys 14) at the MFA2 promoter and throughout the genome. The yeast repressor complex Ssn6-Tup1 represses many genes including MFA2. TUP1 deletion results in constitutive hyperacetylation of histone H3, nucleosome disruption and derepression of gene transcription in Tup1-regulated genes. GG-NER in the MFA2 promoter proceeds more rapidly in tup1Delta alpha-cells compared with wild type, even when transcription is inhibited. We show that elevated histone H3 acetylation levels in the MFA2 promoter in tup1Delta alpha-cells result in Rad7- and Rad16-independent GG-NER, and that Rad16 mediates the ultraviolet-induced acetylation of histone H3, necessary for efficient GG-NER.
Teng
Yumin
Y
Department of Pathology, School of Medicine, Cardiff University, Heath Park, Cardiff, UK.
Liu
Hairong
H
Gill
Hefin W
HW
Yu
Yachuan
Y
Waters
Raymond
R
Reed
Simon H
SH
D000107:Acetylation / Q000528:radiation effects; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D004260:DNA Repair / Q000528:radiation effects*; D004268:DNA-Binding Proteins / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000528:radiation effects; D005800:Genes, Fungal; D016681:Genome, Fungal / Q000235:genetics* / Q000528:radiation effects; D006657:Histones / Q000378:metabolism*; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D010675:Pheromones; D011740:Pyrimidine Dimers / Q000528:radiation effects; D012097:Repressor Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics* / Q000528:radiation effects; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic / Q000528:radiation effects; D014466:Ultraviolet Rays
D004268:DNA-Binding Proteins; D006657:Histones; D008074:Lipoproteins; C095063:MFA2 protein, S cerevisiae; D009687:Nuclear Proteins; D010675:Pheromones; D011740:Pyrimidine Dimers; C063915:RAD7 protein, S cerevisiae; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; C066502:TUP1 protein, S cerevisiae; D000251:Adenosine Triphosphatases; C075467:RAD16 protein, S cerevisiae