title: A GluR1-cGKII interaction regulates AMPA receptor trafficking.
abstract: Trafficking of AMPA receptors (AMPARs) is regulated by specific interactions of the subunit intracellular C-terminal domains (CTDs) with other proteins, but the mechanisms involved in this process are still unclear. We have found that the GluR1 CTD binds to cGMP-dependent protein kinase II (cGKII) adjacent to the kinase catalytic site. Binding of GluR1 is increased when cGKII is activated by cGMP. cGKII and GluR1 form a complex in the brain, and cGKII in this complex phosphorylates GluR1 at S845, a site also phosphorylated by PKA. Activation of cGKII by cGMP increases the surface expression of AMPARs at extrasynaptic sites. Inhibition of cGKII activity blocks the surface increase of GluR1 during chemLTP and reduces LTP in the hippocampal slice. This work identifies a pathway, downstream from the NMDA receptor (NMDAR) and nitric oxide (NO), which stimulates GluR1 accumulation in the plasma membrane and plays an important role in synaptic plasticity.
authors.affiliation: Program in Neuroscience and Physiology, New York University School of Medicine, New York, NY 10016, USA.
authors.forename: Yafell
authors.initials: Y
authors.lastname: Serulle
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.forename: Ipe
authors.initials: I
authors.lastname: Ninan
authors.forename: Daniela
authors.initials: D
authors.lastname: Puzzo
authors.forename: Maria
authors.initials: M
authors.lastname: McCarthy
authors.forename: Latika
authors.initials: L
authors.lastname: Khatri
authors.forename: Ottavio
authors.initials: O
authors.lastname: Arancio
authors.forename: Edward B
authors.initials: EB
authors.lastname: Ziff
mesh_terms: D000818:Animals; D020134:Catalytic Domain; D002460:Cell Line; D002478:Cells, Cultured; D062967:Cyclic GMP-Dependent Protein Kinase Type II; D017869:Cyclic GMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006624:Hippocampus / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009473:Neuronal Plasticity / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009924:Organ Culture Techniques; D010766:Phosphorylation; D011485:Protein Binding / Q000502:physiology; D021381:Protein Transport / Q000187:drug effects / Q000502:physiology; D051381:Rats; D018091:Receptors, AMPA / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D062967:Cyclic GMP-Dependent Protein Kinase Type II; D017869:Cyclic GMP-Dependent Protein Kinases; D004791:Enzyme Inhibitors; D018091:Receptors, AMPA; C569729:PRKG2 protein, human; C569730:Prkg2 protein, mouse; C569732:Prkg2 protein, rat