[Predication of secondary structures and epitopes of fusion protein pp150/MDBP].
The secondary structures of fusion protein pp150/MDBP, including alpha-helix, beta-sheet, turn regions, were analyzed by Garnier-Robson's and Chou-Fasman's methods; the antigenic epitopes of B cells were analysed by using hydrophilicity plot. The results showed that the fusion protein pp150/MDBP might have less alpha-helix, but be rich in beta-sheet and turn regions. The epitopes recognized by B cells may be at 7-56 amino acid residues or adjacent to 137-192 amino acid residues.
Guo
Dadong
D
Key Laboratory for Biotech-drugs, Ministry of Health Shandong Medicinal Biotechnology Centre, Ji'nan 250062, China.
Gao
Xueqin
X
Han
Jinxiang
J
D000595:Amino Acid Sequence; D001665:Binding Sites; D003587:Cytomegalovirus / Q000737:chemistry*; D000939:Epitopes; D006801:Humans; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000737:chemistry* / Q000276:immunology; D017433:Protein Structure, Secondary; D014760:Viral Fusion Proteins / Q000737:chemistry* / Q000276:immunology; D014763:Viral Matrix Proteins / Q000737:chemistry* / Q000276:immunology
D000939:Epitopes; D010750:Phosphoproteins; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins; C056367:pp150 protein, Cytomegalovirus