title: A new water-soluble near-neutral ratiometric fluorescent pH indicator.
abstract: Donor-pi-acceptor fluorene derivative 1c is a near-neutral pH indicator whose pKa of approximately 7.0 was determined by both absorption and fluorescence methods. 1c satisfies important criteria for a sensitive ratiomeric fluorescent pH indicator with a distinctive isoemissive point, good dispersion in cell cytosol, and low cytotoxicity. Furthermore, its 2PA cross section of 100 GM in its neutral form suggests its potential in two-photon fluorescence imaging applications.
authors.affiliation: Department of Chemistry and CREOL, College of Optics and Photonics, University of Central Florida, PO Box 162366, Orlando, Florida 32816-2366, USA.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yao
authors.forename: Katherine J
authors.initials: KJ
authors.lastname: Schafer-Hales
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Belfield
mesh_terms: D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D011874:Radiometry; D012995:Solubility; D014867:Water
chemical_list: D005456:Fluorescent Dyes; D014867:Water