title: Electric impedance assisted micropipette aspiration.
abstract: Micropipette aspiration is a technique to selectively isolate cells from cell cultures using suction pressure. Cells can selectively be isolated one by one from neighboring cells into the micropipette. This paper presents a novel micropipette aspiration system assisted by an impedance measurement system. Furthermore, a method to reduce the adhesion force at a single cell level for a gentler detachment of the cell from a cultivation surface and surrounding cell connections is proposed.
authors.affiliation: Institute of Automation and Control, Tampere University of Technology, 33101 Tampere, Finland.
authors.forename: Katrin
authors.initials: K
authors.lastname: Kovanen
authors.forename: Mikko
authors.initials: M
authors.lastname: Lukkari
authors.forename: Sami
authors.initials: S
authors.lastname: Purmonen
authors.forename: Timo
authors.initials: T
authors.lastname: Ylikomi
authors.forename: Jouko
authors.initials: J
authors.lastname: Viitanen
authors.forename: Pasi J
authors.initials: PJ
authors.lastname: Kallio
mesh_terms: D002468:Cell Physiological Phenomena; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured / Q000166:cytology* / Q000502:physiology*; D017097:Electric Impedance; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D008846:Micromanipulation / Q000379:methods*; D013396:Suction / Q000379:methods*