Influence of nucleoside analogue treatment on telomere length and TRF2 amount in human HL60 cells.
Long-term treatment with 3'-azido-2',3'-dideoxy-guanosine (AZddG) results in reproducible telomere shortening in cultured human HL60 cells. TRF2 protein has been implicated in the protection of chromosome ends. It binds to double-strand repeats and may have an indirect role in protecting the G-rich overhang by recruiting other telomere-binding proteins to the G-tail or by mediating the formation of the telomeric t-loop. Western blot analysis demonstrated no change or a slight increase, of the TRF2 protein level in HL60 cells with AZddG-induced telomere shortening. The effects of nucleoside analogues on TRF2 suggest that it is not telomere length per se, but rather the presence or absence of a protective telomere state, which determines whether senescence ensues.
Liu
Xiaohong
X
Biotechnology Research Center, Teikyo University of Science and Technology and Biosciences, Uenohara, Yamanashi, Japan.
Liu
Jingping
J
Ogawara
Tsukasa
T
Kurashina
Yuichi
Y
Yashi
Ryosuke
R
Saneyoshi
Mineo
M
Yamaguchi
Toyofumi
T
D015224:Dideoxynucleosides / Q000737:chemistry / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D016615:Telomere / Q000187:drug effects* / Q000378:metabolism; D035341:Telomeric Repeat Binding Protein 2 / Q000378:metabolism*
D015224:Dideoxynucleosides; D009687:Nuclear Proteins; C504753:TERF2 protein, human; D035341:Telomeric Repeat Binding Protein 2; C051073:3'-azido-2',3'-dideoxyguanosine