title: Hyperproduction of sebaceous cis-6-hexadecenoic acid by esterase-reduced mutant of Rhodococcus sp. strain.
abstract: cis-6-Hexadecenoic acid is a major component of human sebaceous lipids that is involved in skin self-sterilization and atopic dermatitis amelioration. It can be prepared by hydrolysis of isopropyl cis-6-hexadecenoate produced by resting cells of Rhodococcus sp. strain KSM-MT66. To devise an economical industrial-scale process for the production of this rare fatty acid, we optimized the conditions for growing rhodococcal cells. Mg(2+) and Fe(2+) ions are essential for the efficient production of isopropyl cis-6-hexadecenoate. To further increase the production of isopropyl cis-6-hexadecenoate, we created a mutant strain (T64) with reduced esterase activity by random mutagenesis using UV irradiation of MT66. Under an optimized condition, the mutant T64 produced more than 60 g l(-1) isopropyl cis-6-hexadecenoate in a 4-d cultivation, corresponding to about 52 g l(-1)cis-6-hexadecenoate.
authors.affiliation: Tochigi Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi 321-3497, Japan. araki.hiroyuki@kao.co.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Araki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hagihara
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Takigawa
authors.forename: Nobuharu
authors.initials: N
authors.lastname: Kotani
authors.forename: Yukiharu
authors.initials: Y
authors.lastname: Tsujino
authors.forename: Kenzo
authors.initials: K
authors.lastname: Koike
authors.forename: Shuji
authors.initials: S
authors.lastname: Kawai
authors.forename: Katsuya
authors.initials: K
authors.lastname: Ozaki
authors.forename: Susumu
authors.initials: S
authors.lastname: Ito
mesh_terms: D004950:Esterases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010169:Palmitic Acids / Q000378:metabolism*; D012240:Rhodococcus / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D013045:Species Specificity; D015854:Up-Regulation
chemical_list: D010169:Palmitic Acids; C093821:hexadecenoic acid; D004950:Esterases