title: Mapping quantitative trait loci for canine hip dysplasia in German Shepherd dogs.
abstract: Canine hip dysplasia (CHD) is a common hereditary developmental disease of the coxofemoral joints. CHD is characterized by subluxation of the femoral head and deformation of the acetabulum leading to a painful osteoarthrosis. Analyses of mode of inheritance have shown the involvement of a major gene in expression of CHD in German Shepherd dogs. Thus, a whole genome scan for quantitative trait loci (QTL) was performed in German Shepherd dogs. For this purpose 11 paternal half-sib families, including a total of 459 purebred German Shepherd dogs with sires, dams, and offspring, were genotyped for 261 microsatellites. These markers were equidistantly distributed over all 38 autosomes and the X chromosome with an average marker distance of 11.7 cM. The mean observed heterozygosity of the marker set was 50%. The CHD status for the dogs was scored according to the official rules of the Fédération Cynologique Internationale. At the genome-wide level of significance at p < 0.05, QTL for CHD were located on nine different canine chromosomes: 1, 3, 4, 8, 9, 16, 19, 26, and 33. The minimal QTL regions containing the CHD genes spanned on average 5 Mb with a range between 1 and 8.2 Mb. Chromosome-wide level of significance at p < 0.05 was found for QTL on 19 chromosomes. Further analyses can now be performed to refine these map positions of QTL already identified in German Shepherd dogs.
authors.affiliation: Institute for Animal Breeding and Genetics, University of Veterinary Medicine, Bünteweg 17 p, 30559, Hannover, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Marschall
authors.forename: Ottmar
authors.initials: O
authors.lastname: Distl
mesh_terms: D000077:Acetabulum / Q000002:abnormalities; D000818:Animals; D002874:Chromosome Mapping; D004285:Dogs; D005260:Female; D005269:Femur / Q000002:abnormalities; D005838:Genotype; D006619:Hip Dysplasia, Canine / Q000235:genetics*; D008297:Male; D010641:Phenotype; D040641:Quantitative Trait Loci