title: Determination of procalcitonin concentration using the SphereLight 180 clinical auto-analyzer.
abstract: BACKGROUND
  Procalcitonin (PCT) is a biomarker for the diagnosis of sepsis and bacterial infection diseases.
  
  
  METHODS
  A new fully automated SphereLight PCT (SL-PCT) assay system for PCT concentration in human serum or plasma by using SphereLight 180 (SL180, Olympus Corp.) analyzer was developed. The SL-PCT assay is based on chemiluminescent enzyme immunoassay.
  
  
  RESULTS
  A linear dose response relationship was observed up to 200 ng/ml PCT concentration. The detection limit of PCT concentration was 0.06 ng/ml. Endogenous substances, anticoagulants, sodium fluoride and drugs did not interfere with assay results. There was a good correlation between the present method and the manual method in serum and plasma samples.
  
  
  CONCLUSIONS
  These results indicate that the SL-PCT assay showed good performance in terms of the linearity, detection limit and precision. Use of this PCT measurement may improve the detection of sepsis and infectious disease.
authors.affiliation: Osaka Research Laboratories, Wako Pure Chemical Industries Ltd, Amagasaki, Hyogo, Japan. h-yamada@wakousa.com
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamada
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Matsuda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ushio
authors.forename: Kenji
authors.initials: K
authors.lastname: Nakamura
authors.forename: Shinzo
authors.initials: S
authors.lastname: Kobatake
authors.forename: Shinji
authors.initials: S
authors.lastname: Satomura
authors.forename: Shuji
authors.initials: S
authors.lastname: Matsuura
mesh_terms: D002116:Calcitonin / Q000097:blood*; D015740:Calcitonin Gene-Related Peptide; D006801:Humans; D008027:Light; D013677:Medical Laboratory Science / Q000379:methods*; D011498:Protein Precursors / Q000097:blood*; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000097:blood* / Q000175:diagnosis*
chemical_list: C000606589:CALCA protein, human; D011498:Protein Precursors; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide