SAXS measurement of aggregate of DNA modified gold nanoparticles.
DNA-modified gold nanoparticles exhibit a unique aggregation behaviour that they form aggregation with fully complementary DNA but do not with the terminal-mismatched DNA at certain concentration of added salts. We studied the aggregation structure of the nanoparticles by small angle X-ray scattering (SAXS). The results indicated that the gap between surfaces of gold nanoparticles is nearly two times as long as DNA in B-form. This suggests that the ends of completely double-stranded DNA (dsDNA) conjugated on gold nanoparticles contact or slightly interdigitate with each other in the aggregation structure.
Yamakoshi
Shizuto
S
Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8561, Japan. sizuto@molle.k.u-tokyo.ac.jp
Sakai
Yasuhiro
Y
Shinohara
Yuya
Y
Amemiya
Yoshiyuki
Y
Kanayama
Naoki
N
Takarada
Tohru
T
Maeda
Mizuo
M
Ito
Kohzo
K
D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
D006046:Gold; D004247:DNA