title: (13)C heteronuclear NMR studies of the interaction of cultured neurons and astrocytes and aluminum blockade of the preferential release of citrate from astrocytes.
abstract: Citrate has been identified as a major tricarboxylic acid (TCA) cycle constituent preferentially released by astrocytes. We undertook the present study to examine further the nature of metabolic compartmentation in central nervous system tissues using (13)C-labeled glucose and to provide new information on the influence of aluminum on the metabolic interaction between neurons and astrocytes. Metabolites released into the culture medium from astrocytes and neuron-astrocyte coculture, as well as the perchloric acid extracts of the cells were analyzed using 2D (1)H and (13)C NMR spectroscopy. Astrocytes released citrate into the culture medium and the released citrate was consumed by neurons in coculture. Citrate release by astrocytes was blocked in the presence of aluminum, with progressive accumulation of citrate within the cells. We propose citrate supply is a more efficient energy source than lactate for neurons to produce ATP, especially in the hypoglycemic state on account of it being a direct component of the TCA cycle. Astrocytes may be the cellular compartment for aluminum accumulation as a citrate complex in the brain.
authors.affiliation: University Graduate School of Medical Science, 86 Tottori, 683-8503, Japan. mesh@grape.med.tottori-u.ac.jp
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Meshitsuka
authors.forename: David A
authors.initials: DA
authors.lastname: Aremu
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D002247:Carbon Isotopes; D002417:Cattle; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D019343:Citric Acid / Q000378:metabolism*; D018920:Coculture Techniques; D003470:Culture Media / Q000737:chemistry; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010472:Perchlorates / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D002247:Carbon Isotopes; D003470:Culture Media; D010472:Perchlorates; D019343:Citric Acid; D000535:Aluminum