title: Expression of recombinant proteins in Pichia pastoris.
abstract: Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression. These elements include vectors, host strains, heterologous gene integration into the genome, secretion factors, and the glycosylation profile. In particular, we discuss and evaluate the recent progress in optimizing the fermentation process to improve the yield and stability of expressed proteins. Optimization can be achieved by controlling the medium composition, pH, temperature, and dissolved oxygen, as well as by methanol induction and feed mode.
authors.affiliation: Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, Shanghai, 200233, People's Republic of China.
authors.forename: Pingzuo
authors.initials: P
authors.lastname: Li
authors.forename: Anukanth
authors.initials: A
authors.lastname: Anumanthan
authors.forename: Xiu-Gong
authors.initials: XG
authors.lastname: Gao
authors.forename: Kuppusamy
authors.initials: K
authors.lastname: Ilangovan
authors.forename: Vincent V
authors.initials: VV
authors.lastname: Suzara
authors.forename: Nejat
authors.initials: N
authors.lastname: Düzgüneş
authors.forename: V
authors.initials: V
authors.lastname: Renugopalakrishnan
mesh_terms: D005285:Fermentation; D005822:Genetic Vectors; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000378:metabolism; D008957:Models, Genetic; D010100:Oxygen / Q000378:metabolism; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D013696:Temperature
chemical_list: D011994:Recombinant Proteins; D010100:Oxygen; D000432:Methanol