Electrochemical biosensors based on DNA strand exchange.
Electrochemical biosensors for the detection of specific DNA sequences have been described. The redox-modified DNA sequences were hybridized with the 3'-thiol-modified DNA sequences as capture DNA strand. The resulting duplexes were assembled on a gold electrode surface to make DNA chip. Upon addition of fully complementary DNA or mismatch-containing DNA, the time-dependent changes in redox signal were monitored. Mismatched DNA gave less than 20% change when compared with fully matched DNA under the same conditions. The present assay format is thus applicable in discrimination of mutated DNA from fully matched DNA.
Watanabe
Mariko
M
Department of Materials Science and Chemistry, University of Hyogo, 2167 Shosha, Himeji 671-2201, Japan.
Yoshizumi
Jyun
J
Kumamoto
Satoshi
S
Nakamura
Mitsunobu
M
Maruyama
Atsushi
A
Yamana
Kazushige
K
D000880:Anthraquinones / Q000737:chemistry; D020137:Base Pair Mismatch; D015374:Biosensing Techniques; D004252:DNA Mutational Analysis / Q000379:methods*; D004563:Electrochemistry; D015345:Oligonucleotide Probes / Q000737:chemistry; D010084:Oxidation-Reduction
D000880:Anthraquinones; D015345:Oligonucleotide Probes