Immunotherapy of prostate cancer in a murine model using a novel GnRH based vaccine candidate.
Previous studies with gonadotrophin releasing hormone (GnRH/LHRH) vaccines have shown the usefulness of immunization against this hormone in prostate cancer. To this end, we have generated a completely synthetic peptide modified at position 6 and attached to the 830-844 tetanic toxoid (TT) helper T cell sequence. Through this work we have demonstrated that the GnRHm1-TT molecule was highly immunogenic when it is formulated as an oil-based emulsion adjuvated with Montanide ISA 51. That results correlated directly with testosterone reduction and tumor growth inhibition of the Dunning R3327-H androgen responsive prostate tumor model in rats. GnRHm1-TT, proved to be safe and useful for future clinical trials.
Junco
Jesús A
JA
Department of Cancer, Center for Genetic Engineering and Biotechnology of Camaguey, CP 70100, Apdo 387, Camaguey, Cuba. jesus.junco@cigb.edu.cu
Peschke
Peter
P
Zuna
Ivan
I
Ehemann
Volker
V
Fuentes
Franklin
F
Bover
Eddy
E
Pimentel
Eulogio
E
Basulto
Roberto
R
Reyes
Osvaldo
O
Calzada
Lesvia
L
Castro
María D
MD
Arteaga
Niurka
N
López
Yovisleidis
Y
Garay
Hilda
H
Hernández
Héctor
H
Bringas
Ricardo
R
Guillén
Gerardo E
GE
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000906:Antibodies / Q000097:blood; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000276:immunology*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D007167:Immunotherapy; D008297:Male; D008353:Mannitol / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000276:immunology; D009829:Oleic Acids / Q000008:administration & dosage / Q000276:immunology; D010446:Peptide Fragments / Q000008:administration & dosage / Q000138:chemical synthesis / Q000276:immunology; D011471:Prostatic Neoplasms / Q000276:immunology / Q000401:mortality / Q000517:prevention & control / Q000628:therapy*; D051381:Rats; D013739:Testosterone / Q000097:blood; D013745:Tetanus Toxoid / Q000008:administration & dosage / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
D000276:Adjuvants, Immunologic; D000906:Antibodies; D019496:Cancer Vaccines; D009829:Oleic Acids; D010446:Peptide Fragments; D013745:Tetanus Toxoid; D014614:Vaccines, Synthetic; C477385:montanide ISA 51; D007987:Gonadotropin-Releasing Hormone; D008353:Mannitol; D013739:Testosterone