Novel gel-based rapid test for non-instrumental detection of ochratoxin A in beer.
A rapid easy-to-use immunoassay was optimised for the non-instrumental detection of ochratoxin A (OTA) in beer. The analytical method involves preconcentration on the immunoaffinity layer inside a column followed by direct competitive ELISA detection in the same layer. The visual cut-off value, i.e. the lowest OTA concentration resulting in no colour development, was 0.2 microg L(-1). Assay validation was performed using samples spiked with OTA. Thirty-seven naturally contaminated samples were screened with the gel-based method developed and no false-negative results were obtained. The method described offers a simple, rapid and cost-effective screening tool, thus contributing to better health protection of consumers.
Goryacheva
I Y
IY
Department of Common and Inorganic Chemistry, Chemistry Faculty, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia. goryachevaiy@info.sgu.ru
Basova
E Y
EY
Van Peteghem
C
C
Eremin
S A
SA
Pussemier
L
L
Motte
J-C
JC
De Saeger
S
S
D001515:Beer / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination; D005782:Gels / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D009793:Ochratoxins / Q000032:analysis*; D013997:Time Factors
D005782:Gels; D009793:Ochratoxins; C025589:ochratoxin A