title: Development of stable DNA microarray platforms suitable for quantitative analysis.
abstract: DNA microarray has become one of the most indispensable tools for genome analysis, medical diagnosis and molecular biological studies. In this study, to make DNA microarray more reliable and efficient, we employed additional silanization modification on aminoalkoxysilane-treated surface. In addition, oxanine was used as a linker of DNA probe, which does not require any chemical activation step in probe immobilization. These strategies were practically convenient in DNA microarray fabrication, and the newly developed system showed enhanced stability for the immobilized DNA probes that could be useful for quantitative analysis.
authors.affiliation: Institute of Advanced Energy, Kyoto University, Gokaso, Uji 611-0011, Japan.
authors.forename: Nagendra Kumar
authors.initials: NK
authors.lastname: Kamisetty
authors.forename: Seung Pil
authors.initials: SP
authors.lastname: Pack
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Nonogawa
authors.forename: Kamakshaiah Charyulu
authors.initials: KC
authors.lastname: Devarayapalli
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Kazunari
authors.initials: K
authors.lastname: Yamada
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kodaki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Makino
mesh_terms: D015342:DNA Probes / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011684:Purine Nucleosides / Q000737:chemistry; D012821:Silanes / Q000737:chemistry
chemical_list: D015342:DNA Probes; D011684:Purine Nucleosides; D012821:Silanes; C468766:oxanine