Adhesion of Pseudomonas putida NCIB 9816-4 to a naphthalene-contaminated soil.
The effect of a soil contaminant on the initial adhesion to the soil of a contaminant-degrading soil microorganism in the exponential phase was investigated using naphthalene as the soil contaminant and Pseudomonas putida strain NCIB 9816-4 as the naphthalene-degrading bacteria. P. putida strain DK-1, which is not capable of degrading naphthalene, was used as a control. P. putida NCIB 9816-4 in the exponential phase showed the more adhesion to the soil than that in the stationary phase. In contrast, P. putida DK-1 showed the increased adhesion to the soil when it was in the stationary phase. P. putida NCIB 9816-4 in the exponential phase showed the preferred adhesion to the naphthalene-contaminated soil, whereas the adhesion of P. putida DK-1 was not affected by naphthalene. From the data of surface hydrophobicities of the cells and the soil, the microbial adhesion, especially the initial adhesion to the naphthalene-contaminated soil, takes place through the hydrophobic interaction. We suspect that the surface hydrophobicity of P. putida NCIB 9816-4 in the exponential phase might be increased during the uptake of naphthalene, which caused the preferred adhesion to the naphthalene-contaminated soil.
Hwang
Geelsu
G
Department of Chemical Engineering, Yonsei University, Seoul, South Korea.
Ban
Young-Min
YM
Lee
Chang-Ha
CH
Chung
Chan-Hwa
CH
Ahn
Ik-Sung
IS
D001422:Bacterial Adhesion / Q000502:physiology*; D057927:Hydrophobic and Hydrophilic Interactions; D046529:Microscopy, Electron, Transmission; D009281:Naphthalenes / Q000378:metabolism*; D016958:Pseudomonas putida / Q000302:isolation & purification* / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000302:isolation & purification*
D009281:Naphthalenes; D012989:Soil Pollutants; C031721:naphthalene