title: In vitro differentiation of human mesenchymal stem cells on Ti6Al4V surfaces.
abstract: Long-term stability of arthroplasty prosthesis depends on the integration between the bone tissue and the implanted biomaterials, which requires the contribution of osteoblastic precursors and their continuous differentiation into the osteoblastic phenotype. Classically, these interactions are tested in vitro using mesenchymal stem cells (MSCs) isolated and ex vivo expanded from bone marrow aspirates. Human adipose tissue-derived stromal cells (AMSCs) may be a more convenient source of MSCs, according to their abundance and accessibility, but no data are available on their in vitro interactions with hard biomaterials. The aim of this work is to compare the osteogenic potential of human AMSCs and bone marrow-derived MSCs (BMMSCs) and to evaluate their response to Ti6Al4V alloy in terms of adhesion, proliferation and differentiation features, using the human osteosarcoma cell line SaOS-2 for comparison. The overall results showed that AMSCs have the same ability to produce bone matrix as BMMSCs and that Ti6Al4V surfaces exhibit an osteoinductive action on AMSCs, promoting their differentiation into functional osteoblasts and increasing bone formation. In conclusion, adipose tissue is a promising autologous source of osteoblastic cells with important clinical implications for bone tissue engineering.
authors.affiliation: Department of Internal Medicine, Medical School, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy.
authors.forename: Isabella
authors.initials: I
authors.lastname: Tognarini
authors.forename: Sabina
authors.initials: S
authors.lastname: Sorace
authors.forename: Roberto
authors.initials: R
authors.lastname: Zonefrati
authors.forename: Gianna
authors.initials: G
authors.lastname: Galli
authors.forename: Alessia
authors.initials: A
authors.lastname: Gozzini
authors.forename: Silvia
authors.initials: S
authors.lastname: Carbonell Sala
authors.forename: Giorgia D Zappoli
authors.initials: GD
authors.lastname: Thyrion
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Carossino
authors.forename: Annalisa
authors.initials: A
authors.lastname: Tanini
authors.forename: Carmelo
authors.initials: C
authors.lastname: Mavilia
authors.forename: Chiara
authors.initials: C
authors.lastname: Azzari
authors.forename: Fausto
authors.initials: F
authors.lastname: Sbaiz
authors.forename: Alessandro
authors.initials: A
authors.lastname: Facchini
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Capanna
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Brandi
mesh_terms: D000199:Actins / Q000378:metabolism; D000368:Aged; D000497:Alloys; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000166:cytology; D002448:Cell Adhesion; D002454:Cell Differentiation; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008875:Middle Aged; D010006:Osteoblasts / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D013499:Surface Properties; D014025:Titanium
chemical_list: D000199:Actins; D000497:Alloys; D015415:Biomarkers; D012333:RNA, Messenger; C031462:titanium alloy (TiAl6V4); D014025:Titanium