title: Functional analysis of the role of Fur in the virulence of Pseudomonas syringae pv. tabaci 11528: Fur controls expression of genes involved in quorum-sensing.
abstract: In most bacteria, Fur (ferric uptake regulator) is a crucial global regulator known to operate not only in the regulation of iron homeostasis but also in a variety of other cellular processes. In an effort to characterize the role of Fur in the virulence of plant pathogens, a fur homolog was isolated from Pseudomonas syringae pv. tabaci 11528. Phenotype assays showed that a fur deletion mutant (BL33) constitutively produced siderophore(s) and exhibited decreases in swarming motility as well as the synthesis of tabtoxin and N-acyl homoserine lactones. Consistent with the results of TLC, quantitative real-time RT-PCR of the QS associated genes psyR and psyI demonstrated that Fur up-regulates these genes at the transcriptional level. Finally, the effects of a fur mutation on plant virulence indicated that Fur-regulated traits are relevant to plant-pathogen interactions.
authors.affiliation: Department of Microbiology, College of Natural Science, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Cha
authors.forename: Jun Seung
authors.initials: JS
authors.lastname: Lee
authors.forename: Jeong-Il
authors.initials: JI
authors.lastname: Oh
authors.forename: Jang Won
authors.initials: JW
authors.lastname: Choi
authors.forename: Hyung Suk
authors.initials: HS
authors.lastname: Baik
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D044224:Pseudomonas syringae / Q000472:pathogenicity* / Q000502:physiology*; D053038:Quorum Sensing / Q000502:physiology*; D012097:Repressor Proteins / Q000378:metabolism*; D014026:Nicotiana / Q000382:microbiology* / Q000502:physiology*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial