Rapid and highly sensitive coxsackievirus a indirect immunofluorescence assay typing kit for enterovirus serotyping.
We describe the development and evaluation of an indirect immunofluorescence assay (IFA) kit for rapid and sensitive detection of coxsackievirus A2, -4, -5, -6, and -10. This IFA kit was determined to have 95.9 to 100% sensitivity and 95.8 to 97.2% specificity. It also proved to be beneficial in reducing the number of enteroviruses that are untypeable in the clinical virology laboratory.
Lin
Tsuey-Li
TL
Center for Research and Diagnostic, Centers for Disease Control, Department of Health, Taiwan, 161 Kunyang Street, Nan-Kang, Taipei, Taiwan.
Li
Yi-Syue
YS
Huang
Chiao-Wei
CW
Hsu
Chiu-Chu
CC
Wu
Ho-Sheng
HS
Tseng
Tsan-Chang
TC
Yang
Chen-Fu
CF
D003384:Coxsackievirus Infections / Q000175:diagnosis* / Q000821:virology*; D004770:Enterovirus / Q000145:classification*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity