title: [Construction of NF-kappaB siRNA expression vector].
abstract: OBJECTIVE
  To construct a NF-kappaB siRNA expression vector and to detect the specific silencing effect of the siRNA on the expression of NF-kappaB protein.
  
  
  METHODS
  pcDNA3.1/CT-GFP-TOPO recombinant eukaryotic expression vector and pSilencer 1.0-U6-siRNA-NF-kappaB recombinant vector were constructed respectively. These 2 recombination plasmids were co-transfected into COS-7 cells, and the NF-kappaB silence induced by RNAi was detected by Western blot and the inverted fluorescence microscope.
  
  
  RESULTS
  The levels of NF-kappaB protein in COS-7 cells could be silenced effectively and specifically by pSilencer 1.0-U6-siRNA- NF-kappa recombinant vector. The expression of NF-kappaB protein was reduced gradually with the increase of pSilencer 1.0-U6-siRNA- NF-kappaB recombinant vector,which could be detected by Western blot under the inverted fluorescence microscope.
  
  
  CONCLUSIONS
  NF-kappaB siRNA expression vector is constructed successfully.
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Yunusi
authors.initials: Y
authors.lastname: Kurexi
authors.forename: Guo-hong
authors.initials: GH
authors.lastname: Huang
authors.forename: Mei
authors.initials: M
authors.lastname: Long
authors.forename: Jie
authors.initials: J
authors.lastname: He
authors.forename: Maimaitiaili
authors.initials: M
authors.lastname: Nasier
authors.forename: Yan-ping
authors.initials: YP
authors.lastname: Xia
authors.forename: Wufuer
authors.initials: W
authors.lastname: Hamulati
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D005822:Genetic Vectors; D016328:NF-kappa B / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering; D014162:Transfection
chemical_list: D016328:NF-kappa B; D034741:RNA, Small Interfering