title: Molecular crowding enhances native structure and stability of alpha/beta protein flavodoxin.
abstract: To investigate the consequences of macromolecular crowding on the behavior of a globular protein, we performed a combined experimental and computational study on the 148-residue single-domain alpha/beta protein, Desulfovibrio desulfuricans apoflavodoxin. In vitro thermal unfolding experiments, as well as assessment of native and denatured structures, were probed by using far-UV CD in the presence of various amounts of Ficoll 70, an inert spherical crowding agent. Ficoll 70 has a concentration-dependent effect on the thermal stability of apoflavodoxin (DeltaT(m) of 20 degrees C at 400 mg/ml; pH 7). As judged by CD, addition of Ficoll 70 causes an increase in the amount of secondary structure in the native-state ensemble (pH 7, 20 degrees C) but only minor effects on the denatured state. Theoretical calculations, based on an off-lattice model and hard-sphere particles, are in good agreement with the in vitro data. The simulations demonstrate that, in the presence of 25% volume occupancy of spheres, native flavodoxin is thermally stabilized, and the free energy landscape shifts to favor more compact structures in both native and denatured states. The difference contact map reveals that the native-state compaction originates in stronger interactions between the helices and the central beta-sheet, as well as by less fraying in the terminal helices. This study demonstrates that macromolecular crowding has structural effects on the folded ensemble of polypeptides.
authors.affiliation: Departments of Biochemistry and Cell Biology and Chemistry, Keck Center for Structural Computational Biology, Rice University, Houston, TX 77251, USA.
authors.forename: Loren
authors.initials: L
authors.lastname: Stagg
authors.forename: Shao-Qing
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Margaret S
authors.initials: MS
authors.lastname: Cheung
authors.forename: Pernilla
authors.initials: P
authors.lastname: Wittung-Stafshede
mesh_terms: D001059:Apoproteins / Q000737:chemistry* / Q000187:drug effects; D002021:Buffers; D002942:Circular Dichroism; D003198:Computer Simulation; D045285:Desulfovibrio desulfuricans / Q000737:chemistry; D005362:Ficoll / Q000494:pharmacology; D005418:Flavodoxin / Q000737:chemistry* / Q000187:drug effects; D008958:Models, Molecular; D015394:Molecular Structure; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry; D013816:Thermodynamics
chemical_list: D001059:Apoproteins; D002021:Buffers; D005418:Flavodoxin; D011994:Recombinant Proteins; C026932:apoflavodoxin; D005362:Ficoll