Rapid detection of important human pathogenic Phleboviruses.
BACKGROUND
Rapid diagnostics are not available for several human pathogens in the genus Phlebovirus of the Bunyaviridae.


OBJECTIVE
To develop RT-PCR assays for Sandfly Fever Sicilian virus (SFSV), Sandfly Fever Naples virus (SFNV), Toscana virus (TOSV) and Rift Valley Fever virus (RVFV).


METHODS
RNA standards were generated and used to test the performance of the assays.


RESULTS
A detection limit of 10-100 RNA molecules was determined for the SFSV, TOSV and RVFV assays. The sensitivity of the SFNV assay was not determined. The TOSV and the RVFV assays detected recent isolates from Spain and Africa, respectively.


CONCLUSIONS
The assays should help to improve surveillance of pathogenic Phleboviruses.
Weidmann
Manfred
M
Institute of Virology, University of Göttingen, Kreuzbergring 57, 37075 Göttingen, Germany. mweidma@gwdg.de
Sanchez-Seco
M Paz
MP
Sall
Amadou A
AA
Ly
Peinda Ogo
PO
Thiongane
Yaya
Y
Lô
Modou M
MM
Schley
Heike
H
Hufert
Frank T
FT
D000818:Animals; D002044:Bunyaviridae Infections / Q000821:virology*; D002522:Chlorocebus aethiops; D006801:Humans; D016856:Phlebovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012296:Rift Valley fever virus / Q000235:genetics / Q000302:isolation & purification; D029301:Sandfly fever Naples virus / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013045:Species Specificity; D019914:Taq Polymerase / Q000378:metabolism; D013997:Time Factors; D014709:Vero Cells
D019914:Taq Polymerase