title: Probing the mutation spectrum in E. coli.
abstract: The mutation spectrum, together with mutation frequency, is decisive for the size and nature of genetic diversity. We constructed plasmid-encoded probes for specific detection of each and all of the six base substitutions. Using the set of the probes, we analyzed the mutation spectrumon the plasmids caused by different types of mutagens and mutator enzymes/alleles.
authors.affiliation: Gaduate School of Engineering, Chiba Univeristy, 1-33, Yayoi-cho, Inage-ku, Chiba City, Chiba 263-8522, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Keiichi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Yohei
authors.initials: Y
authors.lastname: Tashiro
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Saito
authors.forename: Daisuke
authors.initials: D
authors.lastname: Umeno
mesh_terms: D004252:DNA Mutational Analysis / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D016296:Mutagenesis; D010957:Plasmids / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001618:beta-Lactamases