Synthesis of novel spirostanic saponins and their cytotoxic activity.
This study was carried out to assess the cytotoxicity of several new synthetic steroidal saponins against the human myeloid leukemia cell lines (HL-60 and U937) and against human melanoma cells (SK-MEL-1). Several diosgenyl glycosides analyzed showed strong cell growth inhibition which was associated with alterations in cell cycle progression and induction of apoptosis. Studies of cytochrome c release and caspase-9 activation suggest a main role of the intrinsic pathway of apoptosis in the mechanism of cytotoxicity caused by this kind of compounds.
Hernández
Juan C
JC
Instituto de Productos Naturales y Agrobiología-C.S.I.C., Antonio González, Av. Astrofísico F. Sánchez 3, 38206 La Laguna, Tenerife, Spain.
León
Francisco
F
Brouard
Ignacio
I
Torres
Fernando
F
Rubio
Sara
S
Quintana
José
J
Estévez
Francisco
F
Bermejo
Jaime
J
D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D053453:Caspase 9; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D045304:Cytochromes c; D004354:Drug Screening Assays, Antitumor; D018922:HL-60 Cells; D006801:Humans; D008545:Melanoma; D012503:Saponins / Q000138:chemical synthesis / Q000494:pharmacology*; D020298:U937 Cells
D000970:Antineoplastic Agents; D012503:Saponins; D045304:Cytochromes c; D053453:Caspase 9