Arabidopsis MALE STERILITY1 encodes a PHD-type transcription factor and regulates pollen and tapetum development.
The Arabidopsis thaliana MALE STERILITY1 (MS1) gene encodes a nuclear protein with Leu zipper-like and PHD-finger motifs and is important for postmeiotic pollen development. Here, we examined MS1 function using both cell biological and molecular biological approaches. We introduced a fusion construct of MS1 and a transcriptional repression domain (MS1-SRDX) into wild-type Arabidopsis, and the transgenic plants showed a semisterile phenotype similar to that of ms1. Since the repression domain can convert various kinds of transcriptional activators to dominant repressors, this suggested that MS1 functioned as a transcriptional activator. The Leu zipper-like region and the PHD motif were required for the MS1 function. Phenotypic analysis of the ms1 mutant and the MS1-SRDX transgenic Arabidopsis indicated that MS1 was involved in formation of pollen exine and pollen cytosolic components as well as tapetum development. Next, we searched for MS1 downstream genes by analyzing publicly available microarray data and identified 95 genes affected by MS1. Using a transgenic ms1 plant showing dexamethasone-inducible recovery of fertility, we further examined whether these genes were immediately downstream of MS1. From these results, we discuss a role of MS1 in pollen and tapetum development and the conservation of MS1 function in flowering plants.
Ito
Takuya
T
Antibiotics Laboratory, RIKEN, Tsukuba 305-0074, Japan.
Nagata
Noriko
N
Yoshiba
Yoshu
Y
Ohme-Takagi
Masaru
M
Ma
Hong
H
Shinozaki
Kazuo
K
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000196:embryology* / Q000235:genetics / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D016350:Leucine Zippers; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D032306:Petunia / Q000187:drug effects / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D051479:Plant Infertility / Q000187:drug effects; D030821:Plants, Genetically Modified; D011058:Pollen / Q000187:drug effects / Q000196:embryology* / Q000235:genetics / Q000648:ultrastructure; D011401:Promoter Regions, Genetic / Q000235:genetics; D017434:Protein Structure, Tertiary; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism*
D029681:Arabidopsis Proteins; C461559:MS1 protein, Arabidopsis; D014157:Transcription Factors; D003907:Dexamethasone