Clean-up method of forchlorfenuron in agricultural products for HPLC analysis.
A simplified method for the determination of forchlorfenuron in agricultural products by HPLC with UV detection was investigated. A chopped sample homogenate from agricultural products was extracted with acetone. The extract was filtrated and concentrated. The residues was loaded onto a Chem Elut column and extracted with ethyl acetate. The crude extract was purified on Oasis HLB and Bond Elut PSA mini-columns using a mixture of methanol and ethyl acetate. Forchlorfenuron was analyzed by HPLC with UV detection (263 nm). HPLC separation was performed on an ODS column with methanol-water as the mobile phase. Recoveries of forchlorfenuron from several agricultural products fortified at the level of 0.1 microg/g were in the range of 87.6-99.5%. The limit of detection (S/N=3) was 0.005 microg/g in the sample.
Kobayashi
Maki
M
Tokyo Metropolitan Institute of Public Health, 3-24-1 Hyakunincho, Shinjuku-ku, Tokyo 169-0073, Japan.
Takano
Ichiro
I
Tamura
Yasuhiro
Y
Tomizawa
Sanae
S
Tateishi
Yukinari
Y
Sakai
Naoko
N
Kamijo
Kyoko
K
Ibe
Akihiro
A
Nagayama
Toshihiro
T
D002623:Chemistry Techniques, Analytical / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D018556:Crops, Agricultural / Q000737:chemistry*; D010671:Phenylurea Compounds / Q000032:analysis*; D010937:Plant Growth Regulators / Q000032:analysis*; D011725:Pyridines / Q000032:analysis*
D010671:Phenylurea Compounds; D010937:Plant Growth Regulators; D011725:Pyridines; C116093:N-(2-chloro-4-pyridyl)-N'-phenylurea