Further stabilization of the double stranded DNA complex by naphthalene diimide derivative having 2,2-dipicolylamine-zinc complex.
Designed naphthalene diimide derivative having 2,2-dipicolylamine (Dpa), NDI-Dpa, could form a Zn(2+) complex and bind to calf thymus DNA with 10-times higher stabilization than that of NDI-Dpa. The obtained SDS-driven dissociation rate constant was 1.0 s(-1), which is smaller than that of NDI-Dpa. These results suggested that NDI-Dpa-Zn can bind to double stranded DNA through threading intercalation and its complex was stabilized by the interaction of Dpa-Zn parts of NDI-Dpa-Zn and phosphodiester parts of dsDNA.
Watanabe
Sadayoshi
S
Department of Applied Chemistry, Faculty of Engineering, Kyushu Institute of Technology, 1-1 Sensui-cyo, Tobata-ku, Kitakyushu-shi, Fukuoka 804-8550, Japan.
Ohtsuka
Keiichi
K
Takenaka
Shigeori
S
D004247:DNA / Q000032:analysis / Q000737:chemistry*; D007094:Imides / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry*; D007700:Kinetics; D009942:Organometallic Compounds / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*
D007094:Imides; D007364:Intercalating Agents; D009942:Organometallic Compounds; D011725:Pyridines; D004247:DNA; C427203:calf thymus DNA