Fluoroquinolone antibiotics having the potential to interfere with fluorescence-based diagnosis.
Fluorescence, both intrinsic and exogenously induced, is being used for diagnosis of abnormal tissue. Excitation wavelengths used by these methods range from 320 to 450 nm. The presence of absorbing or fluorescing drugs is rarely taken into account by practitioners of fluorescence diagnosis and has the potential to yield false-positive or false-negative results. Our aim is to quantify this potential by (1) comparing the quantum yield of fluoroquinolone antibiotics to those of known tissue fluorophores and (2) taking into account drug concentrations in the tissue during treatment. Quantum yields are determined relative to a working standard of Rhodamine 6G in ethanol. The working standard was calibrated against a fluorescein standard. We concentrated our initial efforts on (1) the fluoroquinolone antibiotics, ciprofloxacin, norfloxacin and ofloxacin and (2) the intrinsic tissue fluorophores, NADH, FAD and protoporphyrin IX. When ciprofloxacin, norfloxacin and ofloxacin were excited at wavelengths 310-390 nm, emission occurred from 350 to 650 nm with quantum yields ranging from 0.03 to 0.3. Quantum yields for intrinsic fluorophores excited at their peak absorption wavelengths were 0.02 (NADH, 340 nm), 0.035 (FAD, 450 nm) and 0.087 (protoporphyrin IX, 408 nm). A review of the literature shows that these fluoroquinolones have a large volume of distribution and can be found in high concentrations in almost every organ during a treatment regimen. The product of the drug tissue concentration and quantum yield, which we term the fluorescence effective concentration, is such that it is likely these fluoroquinolones will interfere during fluorescence diagnosis techniques.
Matchette
L Stephanie
LS
FDA/CDRH, Silver Spring, MD, USA.
Agrawal
Anant
A
Pfefer
T Joshua
TJ
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000493:pharmacokinetics; D024841:Fluoroquinolones / Q000737:chemistry* / Q000493:pharmacokinetics; D006801:Humans; D009243:NAD / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D009243:NAD