Putative reaction mechanism of heterologously expressed octopine dehydrogenase from the great scallop, Pecten maximus (L).
cDNA for octopine dehydrogenase (ODH) from the adductor muscle of the great scallop, Pecten maximus, was cloned using 5'- and 3'-RACE. The cDNA comprises an ORF of 1197 nucleotides and the deduced amino acid sequence encodes a protein of 399 amino acids. ODH was heterologously expressed in Escherichia coli with a C-terminal penta His-tag. ODH-5His was purified to homogeneity using metal-chelate affinity chromatography and Sephadex G-100 gel filtration. Recombinant ODH had kinetic properties similar to those of wild-type ODH isolated from the scallop's adductor muscle. Site-directed mutagenesis was used to elucidate the involvement of several amino acid residues for the reaction catalyzed by ODH. Cys148, which is conserved in all opine dehydrogenases known to date, was converted to serine or alanine, showing that this residue is not intrinsically important for catalysis. His212, Arg324 and Asp329, which are also conserved in all known opine dehydrogenase sequences, were subjected to site-directed mutagenesis. Modification of these residues revealed their importance for the catalytic activity of the enzyme. Conversion of each of these residues to alanine resulted in strong increases in K(m) and decreases in k(cat) values for pyruvate and L-arginine, but had little effect on the K(m) and k(cat) values for NADH. Assuming a similar structure for ODH compared with the only available structure of a bacterial opine dehydrogenase, these three amino acids may function as a catalytic triad in ODH similar to that found in lactate dehydrogenase or malate dehydrogenase. The carboxyl group of pyruvate is then stabilized by Arg324. In addition to orienting the substrate, His212 will act as an acid-base catalyst by donating a proton to the carbonyl group of pyruvate. The acidity of this histidine is further increased by the proximity of Asp329.
Müller
Andre
A
Institut für Zoophysiologie, Heinrich-Heine-Universität, Düsseldorf, Germany. andre.mueller@uni-duesseldorf.de
Janssen
Frank
F
Grieshaber
Manfred K
MK
D000594:Amino Acid Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000737:chemistry / Q000378:metabolism; D015153:Blotting, Western; D020134:Catalytic Domain / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009154:Mutation; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D049910:Pecten / Q000201:enzymology* / Q000235:genetics; D011773:Pyruvates / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity
D018076:DNA, Complementary; D011773:Pyruvates; D011994:Recombinant Proteins; D009243:NAD; D001120:Arginine; D000594:Amino Acid Oxidoreductases; C021903:D-octopine dehydrogenase