Development of 2'-o-methyloligoribonucleotide and peptide nucleic acid based artificial ribonucleases.
We have developed 2'-O-methyloligoribonucleotide and peptide nucleic acid (PNA) based artificial ribonucleases (OBAN's), which in presence of zinc or copper ions cleave a potential therapy target in leukemia, an M-BCR/ABL mRNA model. The OBAN's give turnover of substrate and are thus real enzymes. A copper ion based system even gives single site cleavage in the RNA-substrate.
Murtola
Merita
M
Department of Biosciences & Nutrition, Novum, Karolinska Institutet, S-14157 Huddinge, Sweden.
Strömberg
Roger
R
D003300:Copper / Q000737:chemistry; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D009843:Oligoribonucleotides / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012333:RNA, Messenger / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry
D009843:Oligoribonucleotides; D020135:Peptide Nucleic Acids; D012333:RNA, Messenger; D003300:Copper; D016044:Fusion Proteins, bcr-abl; D012260:Ribonucleases; D015032:Zinc