title: Endothelium specific matrilysin (MMP-7) expression in human cancers.
abstract: Over-expression of matrilysin (MMP-7) is predominantly associated with epithelial (pre)malignant cells. In the present study MMP-7 expression is also found in endothelial cells in various human cancer types. Endothelial MMP-7 was associated with CD34 and/or CD105 expression. These immunohistochemical data were confirmed by RT-PCR on VEGF-stimulated endothelial cells. In addition, MMP-7 was also identified in sprouting endothelial cells in vitro. The potential clinical relevance of endothelial MMP-7 was assessed for cervical cancer patients by evaluating the association with overall survival. In contrast to MMP-7 in malignant epithelial cells, MMP-7 expression in endothelial cells showed a significant association with poor survival (LR 5.12, P=0.02, n=30). Our data suggest that MMP-7 is involved in tumor angiogenesis, thereby contributing to malignant growth and hence associated with decreased survival.
authors.affiliation: Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands. c.f.m.sier@lumc.nl
authors.forename: Cornelis F M
authors.initials: CF
authors.lastname: Sier
authors.forename: Lukas J A C
authors.initials: LJ
authors.lastname: Hawinkels
authors.forename: Henry J M A A
authors.initials: HJ
authors.lastname: Zijlmans
authors.forename: Kim
authors.initials: K
authors.lastname: Zuidwijk
authors.forename: Eveline S M
authors.initials: ES
authors.lastname: de Jonge-Muller
authors.forename: Valérie
authors.initials: V
authors.lastname: Ferreira
authors.forename: Roeland
authors.initials: R
authors.lastname: Hanemaaijer
authors.forename: Adri A
authors.initials: AA
authors.lastname: Mulder-Stapel
authors.forename: Gemma G
authors.initials: GG
authors.lastname: Kenter
authors.forename: Hein W
authors.initials: HW
authors.lastname: Verspaget
authors.forename: Arko
authors.initials: A
authors.lastname: Gorter
mesh_terms: D002277:Carcinoma / Q000201:enzymology*; D045744:Cell Line, Tumor; D004727:Endothelium / Q000201:enzymology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020783:Matrix Metalloproteinase 7 / Q000096:biosynthesis*; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic; D018874:Spheroids, Cellular / Q000473:pathology; D013997:Time Factors; D014018:Tissue Distribution; D014471:Umbilical Veins / Q000166:cytology; D002583:Uterine Cervical Neoplasms / Q000201:enzymology*
chemical_list: D020783:Matrix Metalloproteinase 7