title: Endometrial regenerative cells: a novel stem cell population.
abstract: Angiogenesis is a critical component of the proliferative endometrial phase of the menstrual cycle. Thus, we hypothesized that a stem cell-like population exist and can be isolated from menstrual blood. Mononuclear cells collected from the menstrual blood contained a subpopulation of adherent cells which could be maintained in tissue culture for >68 doublings and retained expression of the markers CD9, CD29, CD41a, CD44, CD59, CD73, CD90 and CD105, without karyotypic abnormalities. Proliferative rate of the cells was significantly higher than control umbilical cord derived mesenchymal stem cells, with doubling occurring every 19.4 hours. These cells, which we termed "Endometrial Regenerative Cells" (ERC) were capable of differentiating into 9 lineages: cardiomyocytic, respiratory epithelial, neurocytic, myocytic, endothelial, pancreatic, hepatic, adipocytic, and osteogenic. Additionally, ERC produced MMP3, MMP10, GM-CSF, angiopoietin-2 and PDGF-BB at 10-100,000 fold higher levels than two control cord blood derived mesenchymal stem cell lines. Given the ease of extraction and pluripotency of this cell population, we propose ERC as a novel alternative to current stem cells sources.
authors.affiliation: Bio-Communications Research Institute, Wichita, USA. mxl@brightspot.org
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Meng
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Ichim
authors.forename: Jie
authors.initials: J
authors.lastname: Zhong
authors.forename: Andrea
authors.initials: A
authors.lastname: Rogers
authors.forename: Zhenglian
authors.initials: Z
authors.lastname: Yin
authors.forename: James
authors.initials: J
authors.lastname: Jackson
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Ge
authors.forename: Vladimir
authors.initials: V
authors.lastname: Bogin
authors.forename: Kyle W
authors.initials: KW
authors.lastname: Chan
authors.forename: Bernard
authors.initials: B
authors.lastname: Thébaud
authors.forename: Neil H
authors.initials: NH
authors.lastname: Riordan
mesh_terms: D001769:Blood / Q000378:metabolism; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D004717:Endometrium / Q000166:cytology*; D005260:Female; D006801:Humans; D007621:Karyotyping; D008597:Menstrual Cycle; D018919:Neovascularization, Physiologic; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology*