[The biological properties of VX-2 squamous cell carcinoma model in different sites of rabbit tongue and cervical lymph node metastasis].
OBJECTIVE
To make a VX-2 squamous cell carcinoma model in different sites of rabbit tongue and cervical lymph node metastasis and study its biological properties, in order to offer a research platform for multidisciplinary therapy of tongue carcinoma.


METHODS
Twenty New-Zealand white rabbits were divided into four groups, five in each group, which were implanted with VX-2 squamous cell carcinoma tissue pieces. Tumor growth, volume change, tumor-bearing rate, metastasis rate of cervical lymph node, site and quantity of lymph node metastasis and rabbits' survival period were observed and recorded. The data were analyzed by Runs test, independent-samples test and Chi-square test with SPSS11.5 software package.


RESULTS
Tumor-bearing rate was 75%, 80% in lateral border of tongue and 70% in median line of tongue. Metastasis rate of cervical lymph node was 70%.Tumors in the lateral border of tongue metastasized to the ipsilateral lymph nodes and that in the median line of the tongue metastasized to bilateral lymph nodes. Rabbits' average survival period were (33.25+/-6.20) days in lateral border of tongue and (39.00+/-3.74) days in the median line of tongue. Differences in each site were not statistically significant (P>0.05). Pathologically, tongue carcinoma and cervical lymph node metastasis were both poorly differentiated squamous cell carcinoma.


CONCLUSIONS
With modality of tissue pieces, model of VX-2 squamous cell carcinoma in different sites of rabbit tongue is made successfully. Tumor-bearing rate and metastasis rate of cervical lymph nodes were both higher, rabbits' survival periods were 4 to 6 weeks. Its biological properties are accordance with human tongue carcinoma. The tumor is growing rapidly and metastasizing easily. So it's an ideal animal model to study tongue carcinoma. Supported by Shanghai Leading Academic Discipline Project (Grant No.Y0203).
Shen
Yi
Y
Department of Oral and Maxillofacial Surgery, College of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
Sun
Jian
J
Zhou
Xiao-jian
XJ
Chen
Wan-tao
WT
Xu
Qiu-hua
QH
D000818:Animals; D002294:Carcinoma, Squamous Cell; D004195:Disease Models, Animal; D006801:Humans; D008198:Lymph Nodes; D008207:Lymphatic Metastasis; D009333:Neck; D011817:Rabbits; D014062:Tongue Neoplasms