Effect of alpha-fetoprotein on the count of bone marrow and splenic stromal precursor cells and proliferation of their cultural descendants.
The efficiency of cloning of stromal precursor cell increased more than 2-fold in splenic cultures and more than 3-fold in bone marrow cultures 24 h after injection of Profetal preparation to mice in vivo. The number of nucleated cells did not change in the bone marrow and slightly increased in the spleen. Addition of Profetal in vitro 2-fold decreased the efficiency of stromal precursor cells colony formation in mouse splenic cultures and dose-dependently decreased this process in bone marrow cultures derived from these animals, the maximum (5-fold) inhibitory effect was observed in a dose of 50 microg/ml. Addition of Profetal to cultured human bone marrow fibroblasts did not change the content of stromal fibroblasts in cultures. These data indicate the possibility of indirect effect of alpha-fetoprotein on the number of stromal precursor cell in hemopoietic and lymphoid organs.
Gorskaya
Yu F
YF
Laboratory of Cellular Immunity, N. F. Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow.
Lebedinskaya
O V
OV
Nesterenko
V G
VG
Chailakhyan
R Kh
RKh
Gerasimov
Yu V
YV
Latsinik
N V
NV
Kuralesova
A I
AI
Genkina
E N
EN
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002452:Cell Count; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology; D000509:alpha-Fetoproteins / Q000494:pharmacology*
D000509:alpha-Fetoproteins