[Acid stress increases the activity of superoxide dismutase and catalase in the yeast Saccharomyces cerevisiae].
The effect of acetic acid on survival and activity of superoxide dismutase and catalase in different Saccharomyces cerevisiae strains has been studied. The cell survival decreases with an increase of acetic acid concentration in all strains studied. YBB14 (deltapdr12) and YAK120 (deltawar1) strains are more sensitive to acetate than their parental strain W303-1A. It can be assumed that ABC-transporter Pdr12p and its transcriptional regulator War1p play an important role in the transportation of acetic acid from the cell. Incubation of the yeast with 150-200 mM CH3COOH causes the increase of superoxide dismutase and catalase activities in the wild strain. At the same time, the enzyme activities rise with the increase of time exposure. However, superoxide dismutase and catalase deficit does not decrease yeast survival under conditions used in this study.
Abrat
O B
OB
Semchyshyn
H M
HM
Lushchak
V I
VI
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D019342:Acetic Acid / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D018384:Oxidative Stress / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; C472673:Pdr12 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D002374:Catalase; D013482:Superoxide Dismutase; D019342:Acetic Acid