title: Non-sequence specific RNA inhibition of HIV-1 replication.
abstract: In this study, we investigated an RNA (R-Psi-sgRNA) that suppresses HIV-1 replication. This RNA is expressed by a plasmid vector (pR-Psi-sgRNA-ter) that was constructed accidentally. To examine if this effect is caused by RNA interference, R-Psi-sgRNA was synthesized in vitro and treated with the Dicer enzyme, an important RNase III enzyme for RNA interference. The RNA was cleaved into fragments of approximately 20 nucleotides. We then performed an HIV-1 p24 assay with the RNA fragments to evaluate their effect on HIV-1 replication. HIV-1 replication was suppressed. We are now analyzing the sequences of the RNA fragments.
authors.affiliation: Department of Life and Environmental Science, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan.
authors.forename: Keijiro
authors.initials: K
authors.lastname: Kato
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Habu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takaku
mesh_terms: D019380:Anti-HIV Agents / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D015497:HIV-1 / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D034622:RNA Interference; D043244:Ribonuclease III / Q000378:metabolism; D014779:Virus Replication; D058727:RNA, Small Untranslated
chemical_list: D019380:Anti-HIV Agents; D043244:Ribonuclease III; D058727:RNA, Small Untranslated