Polyamines stabilize left-handed Z-DNA: using X-ray crystallographic analysis, we have found a new type of polyamine (PA) that stabilizes left-handed Z-DNA.
There are many great reports of polyamine stabilization of the Z-DNA by bridge conformation between neighboring, symmetry-related Z-DNA in the packing of crystals. However, polyamine binding to the minor groove of Z-DNA and stabilizing the Z-DNA structure has been rarely reported. We proved that the synthesized polyamines bind to the minor groove of Z-DNA and stabilize the conformation under various conditions, by X-ray crystallographic study. These polyamines consist of a polyamine nano wire structure. The modes of the polyamine interaction were changed under different conditions. It is the first example that the crystals consisted of metal free structure. This finding provides a basis for clarifying B-Z transition mechanics.
Ohishi
Hirofumi
H
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Odoko
Mamiko
M
Grzeskowiak
Kazimierz
K
Hiyama
Yoichi
Y
Tsukamoto
Koji
K
Maezaki
Naoyoshi
N
Ishida
Toshimasa
T
Tanaka
Tetsuaki
T
Okabe
Nobuo
N
Fukuyama
Keiichi
K
Zhou
Da-Yang
DY
Nakatani
Kazuhiko
K
D003198:Computer Simulation; D018360:Crystallography, X-Ray; D043542:DNA, Z-Form / Q000737:chemistry* / Q000648:ultrastructure*; D007536:Isomerism; D008956:Models, Chemical; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D011073:Polyamines / Q000737:chemistry*
D043542:DNA, Z-Form; D011073:Polyamines