title: Monoclonal antibodies reactive with chicken interleukin-17.
abstract: Chicken interleukin-17 (chIL-17) gene was previously characterized through cloning from a chicken intestinal expressed sequence tag (EST) cDNA library. To further investigate the biological properties of chIL-17, six monoclonal antibodies (mAbs) against a bacterially expressed chIL-17 recombinant protein were produced and their binding specificities characterized. Antibodies which were initially selected on the basis of their specific binding reactivity with recombinant chIL-17 in ELISA were further characterized by Western blot analysis. Monoclonal antibodies specific for chIL-17 identified 20 and 21kDa protein bands in the culture supernatant and cell lysate of CU205 cells. These mAbs also recognized specific bands for chIL-17 in the cell lysate from conconavalin A (Con A)-activated, but not from normal splenic lymphocytes. Furthermore, these mAbs detected a 16kDa protein in the lysate of CU205 cells treated with tunicamycin and stained an intracellular protein in CU205 cells in flow cytometric analysis. Together, these results indicate that these new mAbs are specific for chIL-17 and will be a useful tool for structural and immunological studies of IL-17 in poultry.
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Jeongmi
authors.initials: J
authors.lastname: Yoo
authors.forename: Hong H
authors.initials: HH
authors.lastname: Chang
authors.forename: Yeong H
authors.initials: YH
authors.lastname: Bae
authors.forename: Chi-Nam
authors.initials: CN
authors.lastname: Seong
authors.forename: Nong-Hoon
authors.initials: NH
authors.lastname: Choe
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: Jong-Hyeon
authors.initials: JH
authors.lastname: Park
authors.forename: Wongi
authors.initials: W
authors.lastname: Min
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D002461:Cell Line, Transformed; D002645:Chickens / Q000235:genetics / Q000276:immunology*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D006825:Hybridomas / Q000276:immunology; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D020381:Interleukin-17; D011994:Recombinant Proteins; D004247:DNA