title: Characterization of Nocardiopsis beta-1,3-glucanase with additional carbohydrate-binding domains.
abstract: beta-1,3-Glucanase F (BglF) from alkaliphilic Nocardiopsis sp. F96 is a single domain enzyme composed of only a catalytic domain. Chimeric BglFs with some carbohydrate-binding domains were constructed and characterized. By connecting the C-terminal additional domain of beta-1,3-glucanase H from Bacillus circulans IAM1165 and the chitin-binding domain of chitinase J from alkaliphilic Bacillus sp. J813, binding ability and hydrolyzing activity toward insoluble beta-1,3-glucans were both improved.
authors.affiliation: Department of Bioengineering, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Koizumi
authors.forename: Yuya
authors.initials: Y
authors.lastname: Isoda
authors.forename: Kiyoe
authors.initials: K
authors.lastname: Maeda
authors.forename: Sumiko
authors.initials: S
authors.lastname: Masuda
authors.forename: Gunter
authors.initials: G
authors.lastname: Fibriansah
authors.forename: Takashi
authors.initials: T
authors.lastname: Kumasaka
authors.forename: Rie
authors.initials: R
authors.lastname: Yatsunami
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Fukui
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nakamura
mesh_terms: D000192:Actinomycetales / Q000201:enzymology*; D001407:Bacillus / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D043326:Glucan 1,3-beta-Glucosidase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011134:Polysaccharides; D011993:Recombinant Fusion Proteins; D043326:Glucan 1,3-beta-Glucosidase