Using immunoprecipitation to study protein-protein interactions in the Hedgehog-signaling pathway.
The Hedgehog (Hh)-signaling pathway has been intensively studied in the past decade. Increasing evidence suggests that dynamic formation of protein complexes plays a critical role in the organization and regulation of Hh signaling. Immunoprecipitation (IP) is a powerful tool to study protein-protein interactions and has provided important insights into the regulation of Hh signal transduction. Here, we show how to use IP to study protein-protein interactions in the Drosophila Hh-signaling pathway.
Tong
Chao
C
Jiang
Jin
J
D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D015153:Blotting, Western; D002021:Buffers; D004331:Drosophila melanogaster / Q000166:cytology; D000939:Epitopes; D005822:Genetic Vectors / Q000235:genetics; D053823:Hedgehog Proteins / Q000378:metabolism*; D007074:Immunoglobulin G; D047468:Immunoprecipitation / Q000379:methods*; D008969:Molecular Sequence Data; D025941:Protein Interaction Mapping / Q000379:methods*; D015398:Signal Transduction
D000906:Antibodies; D002021:Buffers; D000939:Epitopes; D053823:Hedgehog Proteins; D007074:Immunoglobulin G