Studies on DNA dynamics using 2-N-tert-butylaminoxylpurines.
2-N-tert-Butylaminoxylpurines (1) and (2) were synthesized from 2'-deoxy-6-chloropurine derivative by lithiation strategy. Effects of motion of 1 and 2 on EPR spectra were studied by EPR measurement in sucrose solution at various temperatures. The single stranded and duplexed 15-mers containing 1 showed the clear difference in the EPR spectra to indicate 1 has the potential to accurately study the dynamics of purine residues.
Aso
Mariko
M
Graduate School of Pharmaceutical Sciences, Kyushu University, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Mirc
John Walter
JW
Kurita
Manami
M
Koga
Noboru
N
Suemune
Hiroshi
H
D004247:DNA / Q000737:chemistry*; D003839:Deoxyadenosines / Q000138:chemical synthesis / Q000737:chemistry*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D013696:Temperature
C528357:2-N-tert-butyl-2-oxyl-2'-deoxyguanosine; C528356:2-N-tert-butylaminoxyl-2'-deoxyadenosine; D003839:Deoxyadenosines; D004247:DNA; D003849:Deoxyguanosine