title: Cloning and expression of rat GFAP cDNA in Escherichia coli.
abstract: A cDNA fragment encoding GFAP was amplified by reverse transcription PCR from total mRNA isolated from primary culture of rat astrocytes and cloned for expression in Escherichia coli using pET-28a vector. High level of GFAP expression was confirmed by SDS-PAGE, while immunochemical identity was verified by immunoblotting. The constructed producer strain is a cheap source of GFAP and can be used for diagnostic purposes.
authors.affiliation: Laboratory of Immunochemistry, Department of Biological Psychiatry, V. P. Serbskii Center of Social and Forensic Psychiatry, Moscow.
authors.forename: V P
authors.initials: VP
authors.lastname: Chekhonin
authors.forename: K A
authors.initials: KA
authors.lastname: Pavlov
authors.forename: A N
authors.initials: AN
authors.lastname: Shkoporov
authors.forename: B A
authors.initials: BA
authors.lastname: Yefimov
authors.forename: O I
authors.initials: OI
authors.lastname: Gurina
authors.forename: T B
authors.initials: TB
authors.lastname: Dmitrieva
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D018076:DNA, Complementary; D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger; D011994:Recombinant Proteins