Design, simulation, and optimization of a miniaturized device for size-fractioned DNA extraction.
Fraction collection following electrophoresis has many applications in biology analysis. These assays typically need to identify specific fractions in the separated sample for further processing and require extraction of one or a group of fragments. Unfortunately, the conventional methods involve cumbersome procedures and are not amenable to integration, automation, and extraction of microscale samples. In this work, we present a scheme of chip-based electroelution for the extraction of DNA fragments, which allows the isolation and collection of target DNA fragments in a single device. Both theoretical analysis and experimental results have shown that there are some cross-contaminations from adjacent bands and a little loss of DNA recovery during target DNA extraction with the prototype miniaturized device. In order to improve the purity and yield of the extracted DNA fragment, an optimal channel configuration for DNA recovery miniaturized device was proposed. The simulations and the experimental results are in good agreement and confirm that with the optimized device design a high-efficiency DNA extraction is achieved.
Li
Gang
G
Nanotechnology Laboratory, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, PR China. gang_li@mail.sim.ac.cn
Ran
Rui
R
Zhao
Jian-Long
JL
Xu
Yuan-Sen
YS
D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D004867:Equipment Design; D008904:Miniaturization
D004247:DNA