Genetic variation of papaya ringspot virus in Venezuela.
The genetic variation of papaya ringspot virus (PRSV) in Venezuela was estimated by single strand conformation and nucleotide sequence analyses of two genomic regions of twenty-six isolates. These analyses showed that mutation, virus movement, selection, mixed infections and recombination contributed to shape the genetic variation observed. Phylogenetic analysis indicated that Venezuelan isolates were within a clade composed of isolates from the Americas and Australia. The genetic diversity of these isolates was sufficiently large that it must be taken into account when designing control strategies such as transgenic resistance and cross-protection.
Fernández-Rodríguez
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T
Instituto Venezolano de Investigaciones Científicas (IVIC), Centro de Microbiología y Biología Celular, Laboratorio de Biotecnología y Virología Vegetal, Caracas, Venezuela.
Rubio
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L
Carballo
O
O
Marys
E
E
D029441:Carica / Q000821:virology*; D016000:Cluster Analysis; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014687:Venezuela
D012367:RNA, Viral