title: LBP-1b, LBP-9, and LBP-32/MGR detected in syncytiotrophoblasts from first-trimester human placental tissue and their transcriptional regulation.
abstract: LBP-1b, LBP-9, and LBP-32/MGR (LBP proteins) are transcriptional factors that regulate the expression of the P450 side-chain cleavage enzyme (P450scc) in the human placenta. Placenta contains at least two types of trophoblasts: cytotrophoblasts and syncytiotrophoblasts. P450scc has been detected in syncytiotrophoblasts through all stages of pregnancy. The expression of LBP proteins in different placental stages is unknown. We isolated total RNA from cytotrophoblasts and syncytiotrophoblasts of both first-trimester and full-term human placenta. The mRNA expressions of LBP proteins were detected only in the syncytiotrophoblasts from both first-trimester and full-term placenta. To determine the regulation among LBP proteins, we isolated the 5'-flanking region of one of the LBP proteins (LBP-32/MGR). After determining the transcriptional initiation site by a primer extension assay, we isolated and tested the activity of different lengths of the LBP-32/MGR promoter. A core promoter region for LBP-32/MGR extending from -639 to -184 bp was used to determine the interaction among LBP proteins. We cotransfected LBP-1b and LBP-9 with the LBP-32/MGR promoter and found that both stimulated LBP-32/MGR promoter activity. Our data suggested that an interaction exists among these transcriptional factors at the transcriptional level and provided us with information in our basic understanding of P450scc regulation.
authors.affiliation: Department of Head and Neck Surgery, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030, USA.
authors.forename: Ying C
authors.initials: YC
authors.lastname: Henderson
authors.forename: Mitchell J
authors.initials: MJ
authors.lastname: Frederick
authors.forename: Mary T
authors.initials: MT
authors.lastname: Wang
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Hollier
authors.forename: Gary L
authors.initials: GL
authors.lastname: Clayman
mesh_terms: D001483:Base Sequence; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008954:Models, Biological; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000235:genetics* / Q000378:metabolism; D011263:Pregnancy Trimester, Third / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000502:physiology; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D015533:Transcriptional Activation; D014327:Trophoblasts / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D004268:DNA-Binding Proteins; C518352:GRHL1 protein, human; D012097:Repressor Proteins; C428128:TFCP2L1 protein, human; D014157:Transcription Factors; C085968:UBP1 protein, human; D002786:Cholesterol Side-Chain Cleavage Enzyme