title: Fibrils of different collagen types containing immobilised proteoglycans (PGs) as coatings: characterisation and influence on osteoblast behaviour.
abstract: Collagen, the main organic component of bone, is used as a coating on titanium implants and as a scaffold material in bone tissue engineering. Surface modifications of titanium which promote osteoblast adhesion, proliferation and synthesis of collagen by osteoblasts are desirable. One biomimetic approach is the coating of titanium with collagen in fibrillar form. Other organic components of bone may be bound to fibrils and exert additional effects. In this study, the collagen types I-III were compared regarding their ability to bind the proteoglycans decorin and biglycan, which are found in bone. More collagen was bound to collagen II fibrils than to those of types I and III. Therefore, titanium surfaces were coated with fibrils of collagen type II containing biglycan or decorin or neither to investigate the effect of the proteoglycans on human primary osteoblast behaviour. In addition, the growth factor TGF-beta1 was adsorbed onto surfaces coated with fibrils of collagen type II containing biglycan or decorin or neither to investigate the influence of decorin and biglycan on the effect of TGF-beta1 on osteoblasts. Fibril-bound biglycan and decorin influence primary osteoblast behaviour by themselves. The presence of substrate-bound biglycan or decorin influences the effect of TGF-beta1. These results may be important when designing collagen-based coatings or scaffolds for tissue engineering, including those loaded with growth factors.
authors.affiliation: Max Bergmann Center of Biomaterials, Institute of Material Science, Technische Universität Dresden, Budapester Strasse 27, 01069 Dresden, Germany. Timothy.Douglas@mailbox.tu-dresden.de
authors.forename: T
authors.initials: T
authors.lastname: Douglas
authors.forename: U
authors.initials: U
authors.lastname: Hempel
authors.forename: C
authors.initials: C
authors.lastname: Mietrach
authors.forename: S
authors.initials: S
authors.lastname: Heinemann
authors.forename: D
authors.initials: D
authors.lastname: Scharnweber
authors.forename: H
authors.initials: H
authors.lastname: Worch
mesh_terms: D000368:Aged; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D005260:Female; D024022:Fibrillar Collagens / Q000096:biosynthesis / Q000737:chemistry*; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D010316:Particle Size; D011347:Procollagen / Q000096:biosynthesis / Q000737:chemistry*; D011509:Proteoglycans / Q000737:chemistry*; D051381:Rats; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D024022:Fibrillar Collagens; D011347:Procollagen; D011509:Proteoglycans; D014025:Titanium