Effective two-color SNP typing based on photoligation.
We report a method for the rapid differentiation of nucleic acid sequences with 5-carboxyvinyl-2'-deoxyuridine that allows the simultaneous observation of two colors and facilitates the SNP typing of a heterozygous sample.
Ami
Takehiro
T
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
Fujimoto
Kenzo
K
D020137:Base Pair Mismatch; D002232:Carbocyanines / Q000737:chemistry; D004252:DNA Mutational Analysis / Q000379:methods*; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes / Q000737:chemistry; D010777:Photochemistry; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide
C520201:5-carboxyvinyl-2'-deoxyuridine; D002232:Carbocyanines; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; C089187:cyanine dye 3; C085321:cyanine dye 5; D003857:Deoxyuridine