Effects of pH in rapid-equilibrium enzyme kinetics.
The effects of pH on the rates of enzyme-catalyzed reactions are very important because they yield information on the pKs of acidic groups in the enzymatic site and the various enzyme-substrate complexes. But many enzyme-catalyzed reactions produce or consume hydrogen ions in a way that cannot be explained with pKs. These pH effects extend over the whole pH range of interest. In investigating these effects, the rapid-equilibrium assumption is especially useful because a large number of chemical reactions have to be taken into account. In these calculations, all of the reactions up to the rate-determining reaction are treated with biochemical thermodynamics. Kinetic studies make it possible to determine the number of hydrogen ions consumed in the rate-determining reaction, a number that can be in the range of 0-8. It is shown that the experimental limiting velocity of the forward reaction V(fexp) is equal to 10(npH)V(f), where n is a negative integer and Vf varies with pH in the way determined by the pKs of the enzyme-substrate complex that reacts in the rate-determining reaction. A computer program for the initial reaction velocity makes it possible to investigate the rapid-equilibrium kinetics of enzymatic mechanisms that involve the consumption of hydrogen ions.
Alberty
Robert A
RA
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. alberty@mit.edu
D002384:Catalysis; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013997:Time Factors; D017186:Titrimetry
D004798:Enzymes