title: Electron cryotomography.
abstract: Electron cryotomography is an emerging technique that allows the structures of unique biological objects such as individual macromolecules, viruses, and even small whole cells to be reconstructed in their near-native states in three dimensions (3-D) to an approximate 5-nm resolution. The required instrumentation, sample preparation and limitations, data collection, typical results, and future prospects are summarized briefly.
authors.affiliation: California Institute of Technology, Pasadena, CA, USA.
authors.forename: Gavin E
authors.initials: GE
authors.lastname: Murphy
authors.forename: Grant J
authors.initials: GJ
authors.lastname: Jensen
mesh_terms: D001709:Biotechnology / Q000295:instrumentation / Q000379:methods / Q000639:trends; D020285:Cryoelectron Microscopy / Q000295:instrumentation / Q000379:methods* / Q000639:trends*; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods* / Q000639:trends*; D014054:Tomography / Q000295:instrumentation / Q000379:methods* / Q000639:trends*