Synthesis of 2',5'-oligoadenylate analogs possessing a linker moiety in the place of the second adenosine and their ability to activate human RNase L.
This paper describes the synthesis of 2',5'-oligoadenylate analogs possessing a linker moiety in the place of the second adenosine and their ability to activate human RNase L. Thus, 2-5A analogs (2a-c & 3a-c) possessing -O(CH(2)CH(2))nO- moieties (n = 1 approximately 3) or aromatic ring moieties were prepared. The EC(50) value of the 2-5A analog (2a) incorporating ethylene glycol showed 700 nM in RNase L activation activity.
Nakamura
Takeshi
T
Faculty of Engineering, Gifu University, United Graduate School of Drug Discovery and Medicinal Information Sciences, Gifu University, Yanagido, Gifu 501-1193, Japan.
Ito
Yasutomo
Y
Mochizuki
Masahiko
M
Ueno
Yoshihito
Y
Kitade
Yukio
Y
D000227:Adenine Nucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D000241:Adenosine / Q000737:chemistry; D004722:Endoribonucleases / Q000378:metabolism*; D004789:Enzyme Activation; D005030:Ethylenes / Q000737:chemistry; D006801:Humans; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*
D000227:Adenine Nucleotides; D005030:Ethylenes; D009843:Oligoribonucleotides; C023505:2',5'-oligoadenylate; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease; D000241:Adenosine