Inhibition of airway allergic disease by co-administration of flagellin with allergen.
Bacterial flagellin, which activates Toll-like receptor 5 and cytosolic pattern recognition receptor Ipaf, has a strong immunomodulatory activity. In the present study, we examined whether intranasal co-administration of flagellin with allergen could modulate established airway hyperresponsiveness and Th2 response using an ovalbumin (OVA)-sensitized mouse model. Balb/c mice sensitized with OVA were treated with OVA-flagellin (FlaB) mixture three times at 1-week intervals. Seven days after the final OVA-FlaB administration, the mice were challenged with OVA inhalation, and airway responses and OVA-specific immune responses were evaluated. The OVA-FlaB treatment significantly suppressed OVA-induced airway hyperresponsiveness, airway eosinophilic inflammation, and OVA-specific Th2 cytokine productions in splenocytes. These results indicate that flagellin co-administered with allergen can modulate airway inflammatory response through inhibition of Th2 responses, and flagellin can be considered as a component for allergen-specific immunotherapy.
Lee
Shee Eun
SE
Clinical Vaccine R&D Center, Chonnam National University, Gwangju, South Korea. selee@chonnam.ac.kr
Koh
Youngil I
YI
Kim
Mi-Kwang
MK
Kim
Young Ran
YR
Kim
Soo Young
SY
Nam
Jong Hee
JH
Choi
Yoo Duk
YD
Bae
Soo Jang
SJ
Ko
Young Jong
YJ
Ryu
Hwa-Ja
HJ
Koh
Jeong Tae
JT
Choy
Hyon E
HE
Rhee
Joon Haeng
JH
D000485:Allergens / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001985:Bronchial Provocation Tests; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D016207:Cytokines / Q000378:metabolism; D004804:Eosinophils / Q000166:cytology; D005260:Female; D005408:Flagellin / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology*; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007249:Inflammation / Q000175:diagnosis / Q000628:therapy; D008214:Lymphocytes / Q000166:cytology; D016676:Macrophages, Alveolar / Q000166:cytology; D016210:Methacholine Chloride / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000166:cytology; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology; D012123:Pulmonary Ventilation / Q000187:drug effects; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D012130:Respiratory Hypersensitivity / Q000139:chemically induced / Q000503:physiopathology / Q000628:therapy*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D051198:Toll-Like Receptor 5 / Q000819:agonists
D000485:Allergens; D016207:Cytokines; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D051198:Toll-Like Receptor 5; D016210:Methacholine Chloride; D005408:Flagellin; C416532:flaB flagellin; D010047:Ovalbumin