Rescue of Akabane virus (family Bunyaviridae) entirely from cloned cDNAs by using RNA polymerase I.
Reverse-genetic systems are often used to study different aspects of the viral life cycle. To date, three rescue systems have been developed for the family Bunyaviridae. These systems use T7 RNA polymerase, which is generally used in rescue systems for Mononegavirales. In the present study, we describe a rescue system for Akabane virus (family Bunyaviridae) that uses cDNAs and RNA polymerase I instead of T7 RNA polymerase. The utility of this system was demonstrated by the generation of a mutant with a deletion of the non-structural protein (NSs) on the S RNA segment. These results offer a new option for bunyavirus rescue.
Ogawa
Yohsuke
Y
National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.|Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Sugiura
Keita
K
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Kato
Kentaro
K
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Tohya
Yukinobu
Y
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Akashi
Hiroomi
H
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
D000818:Animals; D000833:Animals, Suckling; D002043:Bunyaviridae / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity; D002044:Bunyaviridae Infections / Q000821:virology*; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D012318:RNA Polymerase I / Q000235:genetics*; D016865:Reassortant Viruses / Q000254:growth & development* / Q000472:pathogenicity; D014779:Virus Replication
D018076:DNA, Complementary; D012318:RNA Polymerase I