Genetic and epidemiological characterization of Middle Point orbivirus, a novel virus isolated from sentinel cattle in northern Australia.
Middle Point orbivirus (MPOV) was isolated in 1998 from a healthy cow pastured at Beatrice Hill farm, Middle Point (formerly Coastal Plains Research Station), 50 km east of Darwin in Australia's Northern Territory. The isolate could not be identified by using conventional serological tests, and electron microscopy indicated that it belongs to the family Reoviridae, genus Orbivirus. Genetic sequencing of segments 2 and 3 revealed that this virus is related to Yunnan orbivirus, an orbivirus known only from China and not previously associated with a vertebrate host. A real-time RT-PCR test was developed to study the epidemiology of this virus in the field. Over 150 previously unidentified viruses isolated from cattle between 1994 and 2006 were positively identified as isolates of MPOV. Serology was used to demonstrate the development of antibody responses to MPOV in cattle from multiple locations across the Northern Territory.
Cowled
Chris
C
CSIRO Livestock Industries, Australian Animal Health Laboratory, East Geelong, Victoria 3220, Australia.
Melville
Lorna
L
Northern Territory Department of Primary Industries, Fisheries and Mines, Berrimah Veterinary Laboratories, Berrimah, Northern Territory 0801, Australia.
Weir
Richard
R
Northern Territory Department of Primary Industries, Fisheries and Mines, Berrimah Veterinary Laboratories, Berrimah, Northern Territory 0801, Australia.
Walsh
Susan
S
Northern Territory Department of Primary Industries, Fisheries and Mines, Berrimah Veterinary Laboratories, Berrimah, Northern Territory 0801, Australia.
Hyatt
Alex
A
CSIRO Livestock Industries, Australian Animal Health Laboratory, East Geelong, Victoria 3220, Australia.
Van Driel
Rosey
R
CSIRO Livestock Industries, Australian Animal Health Laboratory, East Geelong, Victoria 3220, Australia.
Davis
Steven
S
Northern Territory Department of Primary Industries, Fisheries and Mines, Berrimah Veterinary Laboratories, Berrimah, Northern Territory 0801, Australia.
Gubala
Aneta
A
CSIRO Livestock Industries, Australian Animal Health Laboratory, East Geelong, Victoria 3220, Australia.
Boyle
David
D
CSIRO Livestock Industries, Australian Animal Health Laboratory, East Geelong, Victoria 3220, Australia.
D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001315:Australia / Q000453:epidemiology; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology*; D002460:Cell Line; D005260:Female; D005814:Genes, Viral; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D018163:Orbivirus / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D010802:Phylogeny; D012088:Reoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D016415:Sequence Alignment; D016036:Seroepidemiologic Studies
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000914:Antibodies, Viral; D036022:Capsid Proteins