title: Hidden VIM-1 metallo-beta-lactamase phenotypes among Acinetobacter baumannii clinical isolates.
abstract: A total of 87 Acinetobacter baumannii nonrepetitive consecutive clinical isolates were tested for the presence of metallo-beta-lactamases (MBLs). Results of phenotypic assays (MBL Etest, imipenem/imipenem-EDTA combined-disk test, and imipenem/EDTA double-disk synergy test) were negative in all cases, but molecular testing revealed the presence of two bla(VIM-1)-carrying isolates. One isolate had bla(VIM-1) preceded by a weak P1 promoter, and both had inactivated P2 promoters and reduced bla(VIM-1) expression, partially justifying the results revealing hidden MBL phenotypes.
authors.affiliation: Department of Microbiology, Medical School, University of Thessaly, Larissa, Greece.
authors.forename: Alexandros
authors.initials: A
authors.lastname: Ikonomidis
authors.forename: Eleni
authors.initials: E
authors.lastname: Ntokou
authors.forename: Antonios N
authors.initials: AN
authors.lastname: Maniatis
authors.forename: Athanassios
authors.initials: A
authors.lastname: Tsakris
authors.forename: Spyros
authors.initials: S
authors.lastname: Pournaras
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000379:methods; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D047090:beta-Lactams; C417264:VIM-1 metallo-beta-lactamase; D001618:beta-Lactamases