title: Expression and significance of endothelin 1 in spiral ganglion cells of guinea pig.
abstract: OBJECTIVE
  Endothelin 1 has many biological activities including actions in the nervous system. This study aimed to investigate the expression of the endothelin 1 in spiral ganglion cells of guinea pig and its significance in the auditory transmission.
  
  
  METHODS
  Healthy guinea pigs were sacrificed and cardiac perfused with saline followed by 4% paraformaldehyde. Temporal bones were removed and fixed, decalcified in 10% EDTA, embedded in paraffin block and serially sectioned in 5 microm thick slice. Rabbit anti-endothelin 1 polyclonal antibody was used as primary antibody to examine the expression of endothelin 1 in the spiral ganglion by immunohistochemistry.
  
  
  RESULTS
  Endothelin 1 expression was detected in spiral ganglion cells from the basal turn to the apical turn of the cochlea.
  
  
  CONCLUSIONS
  The endothelin 1 presents in spiral ganglions cells of the guinea pig and might play a role in the auditory transmission.
authors.affiliation: Department of Otorhinolaryngology, West China Hospital of Sichuan University, Chengdu 610041, China.
authors.forename: Dingyuan
authors.initials: D
authors.lastname: Xu
authors.forename: Yuedi
authors.initials: Y
authors.lastname: Tang
authors.forename: Shixi
authors.initials: S
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D019332:Endothelin-1 / Q000378:metabolism*; D006168:Guinea Pigs; D007150:Immunohistochemistry; D013136:Spiral Ganglion / Q000166:cytology* / Q000378:metabolism*
chemical_list: D019332:Endothelin-1