title: Synthesis, duplex-forming ability and fluorescent property of OligoDNA bearing silylated fluorophore.
abstract: Novel pyrene derivative conjugated with dimethylsilyl moiety was prepared from bromopyrene. The compound was successfully converted to the corresponding phosphoramidite derivative and incorporated into oligoDNA. In uv-melting study, the resulted fluorescent oligoDNA can discriminate one-base mismatched complement only when the mismatched base is positioned around the middle of the duplex-forming region. On the other hand, the oligoDNA gives marked fluorescence upon binding with the full-matched complement. The intensity of the fluorescent signal of the oligoDNA in a duplex changes depending on the position of the mismatched base of the complement.
authors.affiliation: Graduate Scholl of Chemistry and Chemical Biology, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu City 376-8515, Japan. sinozuka@chem-bio.gunma-u.ac.jp
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozuka
authors.forename: Tohru
authors.initials: T
authors.lastname: Sekiguchi
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Ebara
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Moriguchi
mesh_terms: D020137:Base Pair Mismatch; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D011721:Pyrenes / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D011721:Pyrenes