title: Short-step chemical synthesis of DNA by use of MMTrS group for protection of 5'-hydroxyl group.
abstract: 4-methoxytrithylthio (MMTrS) group was applied for the appropriately protected four canonical nucleosides. We prepared the phosphoroamidite units by use of these nucleosides and developed the synthesis of oligodeoxynucleotides without any acidic treatment. Moreover, the new DNA synthesis protocol was applied to an automated DNA synthesizer for the synthesis of longer oligodeoxynucleotides.
authors.affiliation: Department of life science, Tokyo Institute of Tecnology, Nagatsuta, Midoriku, Yokohama, 226-8501, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Shiraishi
authors.forename: Eri
authors.initials: E
authors.lastname: Utagawa
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohkubo
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
authors.forename: Kohji
authors.initials: K
authors.lastname: Seio
mesh_terms: D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D014320:Triphenylmethyl Compounds / Q000737:chemistry*
chemical_list: C528348:4-methoxytritylthio chloride; D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; D014320:Triphenylmethyl Compounds; C434331:phosphoramidite