RNA interference targeting tNOX attenuates cell migration via a mechanism that involves membrane association of Rac.
tNOX, a tumor-associated NADH oxidase, is a growth-related protein present in transformed cells. In this study, we employed RNA interference (RNAi)-mediated down-regulation of tNOX protein expression to explore the role of tNOX in regulating cell growth in human cervical adenocarcinoma (HeLa) cells. In this first reported use of RNAi to decrease tNOX expression, we found that HeLa cell growth was significantly inhibited by shRNA-knockdown of tNOX. Furthermore, cell migration and membrane association of Rac were decreased concomitantly with the reduction in tNOX protein expression. These results indicate that shRNA targeting of tNOX inhibits the growth of cervical cancer cells, and reduces cell migration via a decrease in the membrane association of Rac. We propose that tNOX is a potential upstream mediator of Rho activation that plays a role in regulating cell proliferation, migration, and invasion.
Liu
Shan-Chi
SC
Graduate Institute of Biomedical Sciences, National Chung Hsing University, Taichung 40227, Taiwan, Republic of China.
Yang
Jaw-Ji
JJ
Shao
Kuo-Ning
KN
Chueh
Pin Ju
PJ
D002462:Cell Membrane / Q000378:metabolism*; D002465:Cell Movement; D020868:Gene Silencing; D018390:Gene Targeting / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference
D009247:NADH, NADPH Oxidoreductases; C431909:tumor-associated NADH oxidase