[Construction of NF-kappaB siRNA expression vector].
OBJECTIVE
To construct a NF-kappaB siRNA expression vector and to detect the specific silencing effect of the siRNA on the expression of NF-kappaB protein.


METHODS
pcDNA3.1/CT-GFP-TOPO recombinant eukaryotic expression vector and pSilencer 1.0-U6-siRNA-NF-kappaB recombinant vector were constructed respectively. These 2 recombination plasmids were co-transfected into COS-7 cells, and the NF-kappaB silence induced by RNAi was detected by Western blot and the inverted fluorescence microscope.


RESULTS
The levels of NF-kappaB protein in COS-7 cells could be silenced effectively and specifically by pSilencer 1.0-U6-siRNA- NF-kappa recombinant vector. The expression of NF-kappaB protein was reduced gradually with the increase of pSilencer 1.0-U6-siRNA- NF-kappaB recombinant vector,which could be detected by Western blot under the inverted fluorescence microscope.


CONCLUSIONS
NF-kappaB siRNA expression vector is constructed successfully.
Kurexi
Yunusi
Y
Department of Biochemistry and Molecular Biology, Xinjiang Medical University, Urumqi 830054, China.
Huang
Guo-hong
GH
Long
Mei
M
He
Jie
J
Nasier
Maimaitiaili
M
Xia
Yan-ping
YP
Hamulati
Wufuer
W
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D005822:Genetic Vectors; D016328:NF-kappa B / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering; D014162:Transfection
D016328:NF-kappa B; D034741:RNA, Small Interfering