Enzymatic incorporation of 2-amino-6-vinylpurine derivative.
Modified nucleotides are incorporated by enzymatic reactions into a DNA oligonulceotide to provide additional functionalities, which has shown potential applications in a wide variety of fields. A large number of modified nucleotide triphosphates have been incorporated to DNA or RNA by polymerases. We have previously described that the sulfide derivatives (1) of 2-amino-6-vinyl purine nucleoside (2, AVP) are activated within duplex to form cross-link toward cytidine selectively at the target site. In this paper, we describe the preparation of the triphosphate derivative of 2'-deoxyribosyl-2-amino-6-(2-methylthioethyl) purine nucleoside (5, dAVP(S Me)-TP), and its enzymatic incorporation to DNA.
Imoto
Shuhei
S
Institute of Multidisciplinary Research for Advanced Materials Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai-city, Miyagi, Japan.
Ali
Md Monsur
MM
Li
Yingfu
Y
Sasaki
Shigeki
S
Nagatsugi
Fumi
F
D004247:DNA / Q000096:biosynthesis / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D011685:Purine Nucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*
C528377:2'-deoxyribosyl-2-amino-6-(2-methylthioethyl)purine nucleoside triphosphate; D011685:Purine Nucleotides; D004247:DNA; D004259:DNA-Directed DNA Polymerase