Identification of boron transporter genes likely to be responsible for tolerance to boron toxicity in wheat and barley.
Tolerance to boron (B) toxicity in cereals is known to be associated with reduced tissue accumulation of B. Genes from roots of B-tolerant cultivars of wheat and barley with high similarities to previously reported B efflux transporters from Arabidopsis and rice were cloned. Expression of these genes was strongly correlated with the ability of tolerant genotypes to lower the concentration of B in roots. The gene from barley located to chromosome 4. Backcross lines containing a B tolerance locus on chromosome 4 showed tolerance in proportion to the level of expression of the transporter gene, whereas those lacking the locus were sensitive to B and had very low levels of gene expression. The results are consistent with a widespread mechanism of tolerance to high B based on efflux of B from root cells.
Reid
Rob
R
School of Earth and Environmental Sciences, University of Adelaide, Adelaide 5005, Australia. robert.reid@adelaide.edu.au
D000222:Adaptation, Physiological / Q000235:genetics*; D001483:Base Sequence; D001895:Boron / Q000378:metabolism* / Q000633:toxicity; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D032461:Chromosomes, Plant; D017931:DNA Primers; D001467:Hordeum / Q000187:drug effects* / Q000378:metabolism; D008969:Molecular Sequence Data; D014908:Triticum / Q000187:drug effects* / Q000378:metabolism
D002352:Carrier Proteins; D017931:DNA Primers; D001895:Boron