title: Fluorescence quenching of the europium tetracycline hydrogen peroxide complex by copper (II) and other metal ions.
abstract: The europium-tetracycline complex [Eu(Tc)] is known to show only weak fluorescence with an emission maximum at 615 nm. On addition of hydrogen peroxide (HP), the strongly fluorescent [Eu(Tc)(HP)] complex is formed, which displays a 15-fold stronger luminescence intensity. This study describes the decrease in luminescence intensity of the [Eu(Tc)(HP)] complex in aqueous solution in the presence of Cu2+, Fe3+, Ag+, Al3+, Zn2+, Co2+, Ni2+, Mn2+, Ca2+, and Mg2+. Static and dynamic quenching can be induced by Cu2+, and these processes were quantified by means of their quenching constants. Stern-Volmer plots were also derived from lifetime imaging measurements accomplished by the rapid lifetime determination (RLD) technique based on microwell plate assays, and also by the time-correlated single photon counting (TCSPC) technique. According to those data, a time-resolved fluorescent method for copper determination can be proposed that is based on dynamic quenching of the [Eu(Tc)(HP)] complex by Cu2+ ions. The response to copper concentrations is linear up to 1.6 micromol L(-1), providing a detection limit of 0.2 micromol L(-1).
authors.affiliation: Department of Analytical Chemistry, University of Granada, Granada 18071, Spain.
authors.forename: Clara
authors.initials: C
authors.lastname: Cano-Raya
authors.forename: María D
authors.initials: MD
authors.lastname: Fernádez Ramos
authors.forename: Luís F
authors.initials: LF
authors.lastname: Capitán Vallvey
authors.forename: Otto S
authors.initials: OS
authors.lastname: Wolfbeis
authors.forename: Michael
authors.initials: M
authors.lastname: Schäferling