Kinetic analysis of the effects of translation enhancers in translation initiation.
Translation initiation is the most important step within a series of protein biosynthesis processes because the incorporation of ribosomes to a mRNA mainly determines efficiencies of translation. In bacteria, translation enhancers located on the 5' upstream of the Shine-Dargalno (SD) sequence on mRNAs are known to accelerate the efficiency of protein biosynthesis. To investigate the role of translation enhancers in translation initiation, we analyzed binding kinetics of a 30S ribosomal subunit to a mRNA immobilized on a 27 MHz quartz-crystal microbalance (QCM). The association constant (Ka) was rather low for the mRNA including a translation enhancer sequence compared with that for the mRNA without translation enhancers. These kinetic parameters suggest that translation enhancers destabilize the ribosome-mRNA complex on an SD sequence to move on the next step of decoding its mRNA.
Takahashi
Shuntaro
S
Department of Biomolecular Engineering, Frontier Collaborative Research Center, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Furusawa
Hiroyuki
H
Shimizu
Yoshihiro
Y
Ueda
Takuya
T
Okahata
Yoshio
Y
D020121:5' Untranslated Regions / Q000737:chemistry*; D001483:Base Sequence; D007700:Kinetics; D008969:Molecular Sequence Data; D010442:Peptide Chain Initiation, Translational; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D038621:Regulatory Sequences, Ribonucleic Acid; D054680:Ribosome Subunits, Small, Bacterial / Q000378:metabolism*
D020121:5' Untranslated Regions; D012333:RNA, Messenger; D038621:Regulatory Sequences, Ribonucleic Acid