Macroporous gel particles as robust macroporous matrices for cell immobilization.
A new design of robust matrices for cell immobilization is described. Macroporous gels (MGs) with immobilized microbial cells were prepared at subzero temperatures and were formed inside a plastic core (so-called, protective housing). Due to the protective housing the macroporous gel particles with immobilized cells can be used in well-stirred bioreactors. High retained activity of yeast (77-92%) and Escherichia coli (50-91%) cells immobilized in MGs after drying and storage in the dried state was due to the high structural stability and heterogeneous porous structure of the MGs.
Plieva
Fatima M
FM
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden. fatima.plieva@biotek.lu.se
Oknianska
Alina
A
Degerman
Eva
E
Mattiasson
Bo
B
D001672:Biocompatible Materials / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D018914:Cells, Immobilized / Q000502:physiology*; D005109:Extracellular Matrix / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D008422:Materials Testing; D010316:Particle Size; D016062:Porosity
D001672:Biocompatible Materials; D005782:Gels