Exogenous application of a lipid transfer protein-jasmonic acid complex induces protection of grapevine towards infection by Botrytis cinerea.
Type I plant lipid transfer proteins (LTPs) are small, basic, cystein-rich proteins involved in plant defense mechanisms. Five type I LTPs isoforms, named VvLTP1, 2, 3, 4 and 5 (Vitis vinifera lipid transfer proteins 1-5) were purified to homogeneity from the culture media of 41B grapevine cell suspension. The full sequence of isoforms 1, 3, 4 and 5 could be determined from mass spectrometry measurements of the enzymatically hydrolyzed proteins and from available VvLTP sequences. Phylogenetic analysis revealed that these proteins form two subgroups, one with isoforms 1 and 4, and the second one with isoforms 3 and 5. The ability of the three most abundant ones (VvLTP1, 4 and 3) to interact with jasmonic acid (JA) was tested by fluorometric studies, showing that VvLTP4 was the most efficient to interact with this oxylipin. Exogenous application of the VvLTP4-JA complex on grapevine plantlets induced a high level (80.3+/-10.05%) of tolerance towards Botrytis cinerea, as compared with control plants (18.65+/-12.13%); whereas plants treated with JA or VvLTP4 alone exhibited a lower protection level (31.04+/-9.72% and 45.52+/-7.51% of protection, respectively). The results are discussed in the context of grapevine defense mechanisms.
Girault
T
T
Biochimie et Biologie Moléculaire Végétales, UMR CNRS-Université de Poitiers 6161, 86022 Poitiers, France.
François
J
J
Rogniaux
H
H
Pascal
S
S
Delrot
S
S
Coutos-Thévenot
P
P
Gomès
E
E
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D020171:Botrytis / Q000254:growth & development*; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005470:Fluorometry; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D011485:Protein Binding; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D027843:Vitis / Q000187:drug effects* / Q000235:genetics / Q000382:microbiology
D002352:Carrier Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D020033:Protein Isoforms; C030363:lipid transfer protein; C011006:jasmonic acid