title: Novel cell seeding system into a porous scaffold using a modified low-pressure method to enhance cell seeding efficiency and bone formation.
abstract: The efficient seeding of cells into porous scaffolds is important in bone tissue engineering techniques. To enhance efficiency, we modified the previously reported cell seeding techniques using low-pressure conditions. In this study, the effects of low pressure on bone marrow-derived stromal cells (BMSCs) of rats and the usefulness of the modified technique were assessed. There was no significant difference found in the proliferative and osteogenic capabilities among various low-pressure (50-760 mmHg, 1-10 min) conditions. To analyze the efficacies of the cell seeding techniques, BMSCs suspended in the plasma of rats were seeded into porous beta-tricalcium phosphate (beta-TCP) blocks by the following three procedures: 1) spontaneous penetration of cell suspension under atmospheric pressure (SP); 2) spontaneous penetration and subsequent low pressure treatment (SPSL), the conventional technique; and 3) spontaneous penetration under low pressure conditions (SPUL), the modified technique. Subsequently, these BMSCs/beta-TCP composites were used for the analysis of cell seeding efficiency or in vivo bone formation capability. Both the number of BMSCs seeded into beta-TCP blocks and the amount of bone formation of the SPUL group were significantly higher than those of the other groups. The SPUL method with a simple technique permits high cell seeding efficiency and is useful for bone tissue engineering using BMSCs and porous scaffolds.
authors.affiliation: Section of Orthopaedic and Spinal Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo 113-8519, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Torigoe
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sotome
authors.forename: Akio
authors.initials: A
authors.lastname: Tsuchiya
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Yoshii
authors.forename: Makoto
authors.initials: M
authors.lastname: Takahashi
authors.forename: Shigenori
authors.initials: S
authors.lastname: Kawabata
authors.forename: Kenichi
authors.initials: K
authors.lastname: Shinomiya
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D001842:Bone and Bones / Q000166:cytology / Q000502:physiology; D002130:Calcium Phosphates / Q000737:chemistry / Q000378:metabolism; D018929:Cell Culture Techniques / Q000295:instrumentation; D002478:Cells, Cultured; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D051381:Rats; D011916:Rats, Inbred F344; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate