Self-assembly of proximity probes for flexible and modular proximity ligation assays.
Proximity ligation assay (PLA) is a recently developed strategy for protein analysis in which antibody-based detection of a target protein via a DNA ligation reaction of oligonucleotides linked to the antibodies results in the formation of an amplifiable DNA strand suitable for analysis. Here we describe a faster and more cost-effective strategy to construct the antibody-based proximity ligation probes used in PLA that is based on the noncovalent interaction of biotinylated oligonucleotides with streptavidin followed by the interaction of this complex with biotinylated antibodies.
Darmanis
Spyros
S
Department of Genetics and Pathology, Uppsala University, Sweden.
Kähler
Anna
A
Spångberg
Lena
L
Kamali-Moghaddam
Masood
M
Landegren
Ulf
U
Schallmeiner
Edith
E
D000906:Antibodies / Q000378:metabolism*; D011088:DNA Ligases / Q000378:metabolism*; D015342:DNA Probes / Q000235:genetics*; D007118:Immunoassay / Q000379:methods*; D015336:Molecular Probe Techniques; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D012680:Sensitivity and Specificity
D000906:Antibodies; D015342:DNA Probes; D011506:Proteins; D011088:DNA Ligases