title: [Study on a novel vascular stent material (titanium oxide, Ti-O) coated with albumin and heparin: is it hemocompatible with fibrinogen].
abstract: The functional hemocompatibility between fibrinogen (FIG) and a novel vascular stent material (Ti-O film fixed with albumin and heparin) was investigated as follows: (1) Preparing the new biologic material (Ti-O) film; (2) Coating albumin and heparin on the Ti-O film; (3) Testing platelets (PL) adsorption; (4) Determining FIG adhesion number by use of enzyme linked immunoassay (ELISA); (5) Implanting the films from the test group of Ti-O film and from the comparison group of stainless steel (SS) film into the left and right femoral arteries respectively in 4 dogs. It was proved that albumin and heparin were fixed on Ti-O film. After 6 months, the femoral arteries of the dogs were resected. In the test group of Ti-O film coated with albumin and heparin, few PL adhered to the coat, their form did not change, and no thrombus was found by scanning electron microscopy; the result was better than that of plain Ti-O film, and was much better than that of SS film. Ti-O maintained normal transformation condition of FIG, and no C terminal of gamma chain in FIG was revealed. As it is known whether the hemocompatibility of a biomaterial is good depends upon its adsorption of FIG, and Ti-O has excellent reaction on albumin and heparin by chemical processes. In this study, the Ti-O film coated with albumin and heparin further reduced the absorption of FIG and PL when compared against the plain Ti-O film. So the Ti-O film coated with albumin and heparin has the insistent and permanent anticoagulant character.
authors.affiliation: Department of Cardiology, Nanjing Drum Tower Hospital Affiliated to School of Medicine Nanjing University, Nanjing 210008, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Song
authors.forename: Xi
authors.initials: X
authors.lastname: Wu
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
authors.forename: Biao
authors.initials: B
authors.lastname: Xu
authors.forename: Fengjuan
authors.initials: F
authors.lastname: Jing
authors.forename: Junying
authors.initials: J
authors.lastname: Chen
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Leng
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.forename: Ansha
authors.initials: A
authors.lastname: Zhao
mesh_terms: D000418:Albumins / Q000737:chemistry*; D000818:Animals; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D004285:Dogs; D005340:Fibrinogen / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods; D011474:Prosthesis Design; D015607:Stents; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000418:Albumins; D020099:Coated Materials, Biocompatible; C009495:titanium dioxide; D005340:Fibrinogen; D006493:Heparin; D014025:Titanium