Photochemical transition of 5-methylcytosine to thymine by DNA photoligation.
We report that deamination coupled with 5-carboxyvinyl-2'-deoxyuridine-mediated photoligation causes the heat-induced transition of 5-methylcytosine to thymine with high selectivity and efficiency without any side reaction. The difference of deamination rate between 5-methylcytosine and cytosine is useful for evaluation of DNA methylation status and the study of gene expression and stability.
Matsumura
Takashi
T
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
Yoshimura
Yoshinaga
Y
Fujimoto
Kenzo
K
D044503:5-Methylcytosine / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000528:radiation effects; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000528:radiation effects; D010777:Photochemistry; D013941:Thymine / Q000737:chemistry*
D009838:Oligodeoxyribonucleotides; D044503:5-Methylcytosine; D004247:DNA; D013941:Thymine