title: Prostanoid receptors in anagen human hair follicles.
abstract: Prostanoid pathway in hair follicle gained closer attention since trichogenic side-effects on hair growth has been observed concomitantly with prostaglandin F(2alpha) receptor (FP) agonist treatment of intraocular pressure. We thus investigated prostanoid receptor distribution in anagen hair follicle and different cell types from hair and skin. Using RT-PCR, Western blot and immunohistochemistry (IHC), we found that all receptors were present in hair follicle. This data shed new light on an underestimated complex network involved in hair growth control. Indeed most of these receptors showed a wide spectrum of expression in cultured cells and the whole hair follicle. Using IHC, we observed that expression of prostaglandin E(2) receptors (EP(2), EP(3), EP(4)), prostaglandin D(2) receptor (DP(2)), prostanoid thromboxane A(2) receptor (TP) and to a lesser extent EP(1) involved several hair follicle compartments. On the opposite, Prostaglandin I(2) receptor (IP) and DP(1) were more specifically expressed in hair cuticle layer and outer root sheath (ORS) basal layer, respectively. FP expression was essentially restricted to ORS companion layer and dermal papilla (DP). Although extracting a clear functional significance from this intricate network remains open challenge, FP labelling, i.e. could explain the biological effect of PGF(2alpha) on hair regrowth, by directly modulating DP function.
authors.affiliation: L'Oréal Recherche, Clichy, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Colombe
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Michelet
authors.forename: Bruno Alain
authors.initials: BA
authors.lastname: Bernard
mesh_terms: D001706:Biopsy; D002478:Cells, Cultured; D005260:Female; D018859:Hair Follicle / Q000254:growth & development* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008875:Middle Aged; D044006:Receptors, Epoprostenol / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism; D011982:Receptors, Prostaglandin / Q000378:metabolism*; D018078:Receptors, Prostaglandin E / Q000378:metabolism; D058307:Receptors, Prostaglandin E, EP2 Subtype; D058308:Receptors, Prostaglandin E, EP3 Subtype; D058309:Receptors, Prostaglandin E, EP4 Subtype; D044244:Receptors, Thromboxane A2, Prostaglandin H2 / Q000378:metabolism; D012535:Scalp / Q000473:pathology
chemical_list: C545644:PTGER2 protein, human; C545647:PTGER3 protein, human; C545650:PTGER4 protein, human; D044006:Receptors, Epoprostenol; D011971:Receptors, Immunologic; D011982:Receptors, Prostaglandin; D018078:Receptors, Prostaglandin E; D058307:Receptors, Prostaglandin E, EP2 Subtype; D058308:Receptors, Prostaglandin E, EP3 Subtype; D058309:Receptors, Prostaglandin E, EP4 Subtype; D044244:Receptors, Thromboxane A2, Prostaglandin H2; C036293:prostaglandin D2 receptor