Structural characterization of supercoiled DNA containing a minisatellite repeat that has polypurine/polypyrimidine stretch.
The pUC19 derivative pAL79 contains six tandem repeats of a 30-bp unit sequence. Supercoiled pAL79 molecules show aberrant electrophoretic mobility under acidic conditions, and microscopic analysis suggests pAL79 molecules conform to tight plectonemes when deposited on a substrate under acidic conditions. This may mean that a short inserted DNA sequence affects the global topology of plasmid DNA.
Kato
Mikio
M
Department of Biological Science, School of Science, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, Sakai 599-8531 Japan. mkato@b.s.osakafu-u.ac.jp
Haku
Takeshi
T
Yamauchi
Takeshi
T
Sugiyama
Shigeru
S
Nagayama
Kuniaki
K
Shimizu
Nobuyoshi
N
D004278:DNA, Superhelical / Q000737:chemistry* / Q000648:ultrastructure; D015180:Electrophoresis, Gel, Two-Dimensional; D018598:Minisatellite Repeats; D010957:Plasmids / Q000737:chemistry; D011687:Purines / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry
D004278:DNA, Superhelical; D011687:Purines; D011743:Pyrimidines