Biosensing based on two-photon fluorescence measurements through optical fibers.
We have performed two-photon fluorescence detection in a new scheme in which femtosecond laser pulses were delivered thorugh an optical fiber for nonlinear excitation and the emitted fluorescence was collected through the same fiber. Single-mode fibers were determined to give higher detection efficiency than multimode fibers, consistent with theoretical considerations. The utility of fiber-optic sensing based on two-photon fluorescence detection was proved by an experiment that measured the uptake of a targeted drug delivery agent into cultured cancer cells.
Ye
Jing Yong
JY
Myaing
Mon Thiri
MT
Norris
Theodore B
TB
Thomas
Thommey
T
Baker
James
J