Spirolide toxin profile of Adriatic Alexandrium ostenfeldii cultures and structure elucidation of 27-hydroxy-13,19-didesmethyl spirolide C.
This paper reports on the determination of the toxin profile of batch cultures of Alexandrium ostenfeldii established from water samples collected along the Emilia-Romagna coasts (Italy) in November 2003. Combined use of LC-MS/MS and 1D and 2D NMR techniques led us to identify 13,19-didesmethylspirolide C and 13-desmethylspirolide C as products of the alga and to highlight the occurrence of a new component, 27-hydroxy-13,19-didesmethylspirolide C, whose structure was fully elucidated by NMR. MS data for a number of minor spirolides are also reported.
Ciminiello
Patrizia
P
Dipartimento di Chimica delle Sostanze Naturali, Università di Napoli Federico II, via D. Montesano, 49, 80131, Napoli, Italy. ciminiel@unina.it
Dell'Aversano
Carmela
C
Fattorusso
Ernesto
E
Forino
Martino
M
Grauso
Laura
L
Tartaglione
Luciana
L
Guerrini
Franca
F
Pistocchi
Rossella
R
D000818:Animals; D004141:Dinoflagellida / Q000737:chemistry*; D007558:Italy; D008387:Marine Toxins / Q000737:chemistry / Q000493:pharmacokinetics*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D013141:Spiro Compounds / Q000737:chemistry / Q000493:pharmacokinetics*
C526834:27-hydroxy-13,19-didesmethyl spirolide C; D008387:Marine Toxins; D013141:Spiro Compounds; C429216:spirolide C