Elucidating global epidemiology of Burkholderia multivorans in cases of cystic fibrosis by multilocus sequence typing.
Burkholderia multivorans is a prominent B. cepacia complex (BCC) species causing infection in people with cystic fibrosis. Despite infection control measures being introduced to reduce the spread of BCC there is a continued emergence of infections by B. multivorans. Our objective was to analyze a global collection of B. multivorans isolates, comparing those from environmental and clinical sources with those from reported outbreaks. Multilocus sequence typing (MLST) was performed on 107 B. multivorans isolates to provide a detailed analysis of the global population biology of this species. MLST resolved 64 B. multivorans sequence types. Twelve of these were globally distributed and associated with human infection; two of these (ST-21 and ST-375) were also composed of environmental isolates. These global lineages included strains previously linked to large outbreaks (e.g., French epidemic clone ST-16). Though few environmental isolates of B. multivorans were available for analysis, of six strains identified, three were identical to strains recovered from cystic fibrosis (CF) infection. Although the ability of B. multivorans to cause CF outbreaks is known, our report here concerning the existence of globally distributed B. multivorans CF strains is a new observation for this emerging B. cepacia complex pathogen and suggests that certain strain types may be better adapted to human infection than others. Common transmission-associated risk factors were not obviously linked to the globally distributed strains; however, the overlap in strains recovered from water environments, industrial products, and human infection suggests that environmental sources may be an important reservoir for infection with B. multivorans.
Baldwin
Adam
A
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom. adam.baldwin@warwick.ac.uk
Mahenthiralingam
Eshwar
E
Drevinek
Pavel
P
Pope
Chris
C
Waine
David J
DJ
Henry
Deborah A
DA
Speert
David P
DP
Carter
Phil
P
Vandamme
Peter
P
LiPuma
John J
JJ
Dowson
Chris G
CG
D015373:Bacterial Typing Techniques / Q000379:methods; D019121:Burkholderia Infections / Q000453:epidemiology* / Q000382:microbiology*; D042602:Burkholderia cepacia complex / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D003550:Cystic Fibrosis / Q000150:complications*; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D004783:Environmental Microbiology; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D004269:DNA, Bacterial