[Determination of chyodeoxycholic acid in Bile Arisaema by HPLC-ELSD].
OBJECTIVE
To develop an HPLC method for the determination of hyodeoxycholic acid in Bile Arisaema.


METHODS
The methanol extracts were separated on a Diamonsil ODS column eluted at 35 degrees C with a mixture of methanol-water-glacial acetic acid (75:25:0.01) at flow-rate 1.0 mL x min(-1). The drift-tube temperature of ELSD was 85 degrees C, and the flow rate of air was 3.0 L x min(-1).


RESULTS
Hyodeoxycholic acid in methanol extract was well separated, the relationship of logarithms of injection amount and peak area was linear (r = 0.9998) over the range of 2.4-24 microg. The average recovery was 97.1% (RSD 2.9%, n=5).


CONCLUSIONS
The method could be used for quality control of Bile Arisaema.
He
Yan
Y
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhang
Yong-xin
YX
Zhao
Hui-dong
HD
Zhang
Qi-wei
QW
D000818:Animals; D031057:Arisaema / Q000737:chemistry*; D001646:Bile / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003840:Deoxycholic Acid / Q000032:analysis*; D004338:Drug Combinations; D006358:Hot Temperature; D008027:Light; D008516:Medicine, Chinese Traditional / Q000592:standards; D035281:Plant Tubers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012542:Scattering, Radiation
D004338:Drug Combinations; D003840:Deoxycholic Acid; C010471:hyodeoxycholic acid