Immunoproteomics of extracellular proteins of Chinese virulent strains of Streptococcus suis type 2.
Streptococcus suis type 2 (SS2) is a porcine zoonotic pathogen with worldwide distribution, and lacking suitable vaccine and virulent maker were bottleneck to control this infection. An immunoproteomic assay was used to identify antigenic proteins from the total extracellular proteins of the virulent Chinese SS2 strain ZY05719. The convalescent serum of a specific pathogen free (SPF) mini-pig recognized nine protein spots on PVDF membrane. Antigenic proteins on a duplicate gel, as well as those with a similar placement of extracellular proteins from another virulent strain (HA9801) and an avirulent strain (T15) on 2-D gels, were excised and identified by MALDI-TOF-MS. PMF of the protein spots were performed using the MASCOT server. Two proteins were found in all three strains. Comparative proteomic analysis between the two virulent strains and the avirulent strain revealed nine differential proteins, eight of which were successfully identified. Genes for six of the differentially expressed proteins were found in both virulent strains, and of those were present in the avirulent stain.
Zhang
Wei
W
Key Laboratory of Animal Disease Diagnostic & Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China.
Lu
Cheng Ping
CP
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D002681:China; D015180:Electrophoresis, Gel, Two-Dimensional; D005798:Genes, Bacterial; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017011:Streptococcus suis / Q000235:genetics / Q000472:pathogenicity*; D013552:Swine; D013556:Swine, Miniature / Q000382:microbiology*; D014774:Virulence
D001426:Bacterial Proteins; D021382:Protein Sorting Signals