title: Particle size determination in aluminum hydroxide suspensions using near-infrared transmittance spectroscopy.
abstract: A new method for particle size determination in polystyrene and aluminum hydroxide suspensions using near-infrared transmittance spectroscopy is described. Mono-dispersed polystyrene particle size standards were used to establish the calibration model. The particle sizes used in the study are similar to the wavelength range of 700-1300 nm, where light scattering is wavelength dependent. The wavelength dependency of near-infrared (NIR) absorbance is found to be linear with the particle size when the analysis is based on the same spectrum starting point (the same absorbance at 700 nm). Partial least squares regression (PLSR) is applied to model this linear relationship. Compared to laser diffraction (LD) the NIR method has similar accuracy and precision in the measurement of particles with a uniform size. For a sample containing multiple sizes of particles, the mean size measured by the NIR method is shown to be weighted by the particle mass. The application of the model to aluminum hydroxide suspension shows that the NIR method is suitable for the detection of particle size changes during the production process and storage. The advantages of the NIR method are that no knowledge of the refractive index and the concentration of a sample are necessary and that the method is fast and easy to operate.
authors.affiliation: Biochemistry and Nutrition Group, BioCentrum-DTU, Søltofts Plads, Technical University of Denmark, Kgs. Lyngby, Denmark.
authors.forename: Xuxin
authors.initials: X
authors.lastname: Lai
authors.forename: Yiwu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Henrik
authors.initials: H
authors.lastname: Ipsen
authors.forename: Susanne
authors.initials: S
authors.lastname: Jacobsen
authors.forename: Jørgen N
authors.initials: JN
authors.lastname: Larsen
authors.forename: Henning
authors.initials: H
authors.lastname: Løwenstein
authors.forename: Ib
authors.initials: I
authors.lastname: Søndergaard
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry*; D000536:Aluminum Hydroxide / Q000737:chemistry*; D016014:Linear Models; D010316:Particle Size; D015203:Reproducibility of Results; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D000276:Adjuvants, Immunologic; D000536:Aluminum Hydroxide