Identification in the mould Hypocrea jecorina of a gene encoding an NADP(+): d-xylose dehydrogenase.
A gene coding for an NADP(+)-dependent d-xylose dehydrogenase was identified in the mould Hypocrea jecorina (Trichoderma reesei). It was cloned from cDNA, the active enzyme was expressed in yeast and a histidine-tagged enzyme was purified and characterized. The enzyme had highest activity with d-xylose and significantly smaller activities with other aldose sugars. The enzyme is specific for NADP(+). The K(m) values for d-xylose and NADP(+) are 43 mM and 250 microM, respectively. The role of this enzyme in H. jecorina is unclear because in this organism d-xylose is predominantly catabolized through a path with xylitol and d-xylulose as intermediates and the mould is unable to grow on d-xylonic acid.
Berghäll
Suvi
S
Hilditch
Satu
S
Penttilä
Merja
M
Richard
Peter
P
D000449:Aldehyde Reductase / Q000235:genetics* / Q000378:metabolism*; D018076:DNA, Complementary / Q000302:isolation & purification; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D035901:Hypocrea / Q000201:enzymology* / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013379:Substrate Specificity; D014994:Xylose / Q000378:metabolism
D018076:DNA, Complementary; D005656:Fungal Proteins; D011994:Recombinant Proteins; D014994:Xylose; D000449:Aldehyde Reductase; C023142:polyol dehydrogenase (NADP)