title: RP-HPTLC densitometric determination and validation of vanillin and related phenolic compounds in accelerated solvent extract of Vanilla planifolia*.
abstract: A simple, fast and sensitive RP-HPTLC method is developed for simultaneous quantitative determination of vanillin and related phenolic compounds in ethanolic extracts of Vanilla planifolia pods. In addition to this, the applicability of accelerated solvent extraction (ASE) as an alternative to microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE) and Soxhlet extraction was also explored for the rapid extraction of phenolic compounds in vanilla pods. Good separation was achieved on aluminium plates precoated with silica gel RP-18 F(254S) in the mobile phase of methanol/water/isopropanol/acetic acid (30:65:2:3, by volume). The method showed good linearity, high precision and good recovery of compounds of interest. ASE showed good extraction efficiency in less time as compared to other techniques for all the phenolic compounds. The present method would be useful for analytical research and for routine analysis of vanilla extracts for their quality control.
authors.affiliation: Natural Plant Products Division, Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India.
authors.forename: Upendra Kumar
authors.initials: UK
authors.lastname: Sharma
authors.forename: Nandini
authors.initials: N
authors.lastname: Sharma
authors.forename: Ajai Prakash
authors.initials: AP
authors.lastname: Gupta
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.forename: Arun Kumar
authors.initials: AK
authors.lastname: Sinha
mesh_terms: D001547:Benzaldehydes / Q000032:analysis* / Q000737:chemistry*; D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry; D057927:Hydrophobic and Hydrophilic Interactions; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis* / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D011312:Pressure; D012997:Solvents; D031669:Vanilla / Q000737:chemistry*
chemical_list: D001547:Benzaldehydes; D010636:Phenols; D010936:Plant Extracts; D012997:Solvents; C100058:vanillin