title: Chemical synthesis of a very long RNA oligomer, a 110mer precursor-miRNA candidate, with 2-cyanoethoxymethyl (CEM) as the 2'-O-protecting group.
abstract: A long RNA oligomer, a 110mer with the sequence of a precursor-miRNA candidate, has been chemically synthesized in a single synthesizer run by means of standard automated phosphoramidite chemistry. The synthetic method involved the use of 2-cyanoethoxymethyl (CEM), a 2'-hydroxyl protecting group recently developed in our laboratory. We confirmed the identity of the synthetic 110mer by MALDI-TOF mass spectrometry, as well as HPLC, electrophoretic methods, RNase-digestion experiments, and its in vitro gene-silencing activity. The chemical synthesis of RNA oligomers of more than 100 nucleotides, which has until now been extremely difficult, can be practically realized by the CEM method.
authors.affiliation: Discovery Research Laboratories, Nippon Shinyaku Co. Ltd., 3-14-1 Sakura, Tsukuba City, Ibaraki 305-0003, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Masuda
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Shiba
authors.forename: Naoki
authors.initials: N
authors.lastname: Watanabe
authors.forename: Kazuchika
authors.initials: K
authors.lastname: Takagaki
authors.forename: Kouichi
authors.initials: K
authors.lastname: Ishiyama
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Ohgi
authors.forename: Junichi
authors.initials: J
authors.lastname: Yano
mesh_terms: D001671:Biochemistry / Q000379:methods; D005019:Ethyl Ethers / Q000737:chemistry*; D008738:Methyl Ethers / Q000737:chemistry*; D035683:MicroRNAs / Q000138:chemical synthesis* / Q000737:chemistry; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D034622:RNA Interference; D012322:RNA Precursors / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: C522071:2-cyanoethyl chloromethyl ether; D005019:Ethyl Ethers; D008738:Methyl Ethers; D035683:MicroRNAs; D009843:Oligoribonucleotides; D012322:RNA Precursors