[Experimental gene therapy of keloid in vivo using recombinant adenovirus carrying Fas gene].
OBJECTIVE
To observe the gene therapeutic effects of two kinds of prepared recombinant adenovirus coding for Fas gene on keloid in nude mouse model.


METHODS
Keloid tissue was transplanted into athymic nude mice subcutaneously to establish the animal model of keloid. Forty-five days after the model had been established, the recombinant adenovirus vectors carrying Fas gene [Ad-Fas (B) or Ad-Fas (T)] or empty vector were injected into the keloid tissues. One day after that, anti-Fas monoclonal antibody (FasMcab) was injected in the keloid tissues. And 7 days after, the animals were sacrificed and changes of the keloid tissues were observed generally and by pathological and electronic microscopic examinations.


RESULTS
The keloid tissues shrunk slightly in the animals injected adenovirus only. The keloid tissues diminished significantly in the animals after injection of Ad-Fas (T) or Ad-Fas (B) followed by FasMcab. Destroyed structure of keloid tissue was found with HE stain. Obvious apoptosis was testified with the electronic microscope.


CONCLUSIONS
Gene therapy of keloid in vivo with recombinant adenovirus carrying Fas gene is effective, and it maybe a potential therapy for keloid.
Lu
Feng
F
Department of Plastic Surgery, Nanfang Hospital of Nanfang Medical University, Guangzhou 510515, China.
Gao
Jian-Hua
JH
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D007627:Keloid / Q000473:pathology / Q000628:therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D014162:Transfection; D019014:fas Receptor / Q000235:genetics*
D019014:fas Receptor