Silver staining DNA in polyacrylamide gels.
This protocol describes a simple silver staining method used to visualize DNA fragments and other organic molecules with unsurpassed detail following traditional polyacrylamide gel electrophoresis (PAGE). Sensitivity rivals radioisotopic methods and DNA in the picogram range can be reliably detected. The described protocol is fast (approximately 1 h) and is implemented using readily available chemicals and materials. To achieve the sensitivity and visual clarity expected, quality reagents and clean handling are important. The updated protocol described here is based on the widely used method of Bassam et al. (1991), but provides improved image contrast and less risk of staining artefacts.
Bassam
Brant J
BJ
Corbett Life Science, Brisbane Technology Park, 42 McKechnie Drive, Eight Mile Plains, Queensland 4113, Australia.
Gresshoff
Peter M
PM
D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005557:Formaldehyde / Q000737:chemistry; D016622:Silver Staining / Q000379:methods*
D005557:Formaldehyde; D004247:DNA