Non-sequence specific RNA inhibition of HIV-1 replication.
In this study, we investigated an RNA (R-Psi-sgRNA) that suppresses HIV-1 replication. This RNA is expressed by a plasmid vector (pR-Psi-sgRNA-ter) that was constructed accidentally. To examine if this effect is caused by RNA interference, R-Psi-sgRNA was synthesized in vitro and treated with the Dicer enzyme, an important RNase III enzyme for RNA interference. The RNA was cleaved into fragments of approximately 20 nucleotides. We then performed an HIV-1 p24 assay with the RNA fragments to evaluate their effect on HIV-1 replication. HIV-1 replication was suppressed. We are now analyzing the sequences of the RNA fragments.
Kato
Keijiro
K
Department of Life and Environmental Science, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan.
Habu
Yuichiro
Y
Takaku
Hiroshi
H
D019380:Anti-HIV Agents / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D015497:HIV-1 / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D034622:RNA Interference; D043244:Ribonuclease III / Q000378:metabolism; D014779:Virus Replication; D058727:RNA, Small Untranslated
D019380:Anti-HIV Agents; D043244:Ribonuclease III; D058727:RNA, Small Untranslated