Involvement of Annexin A2 in p53 induced apoptosis in lung cancer.
Tumor suppressor p53 plays important roles in cell cycle regulation, apoptosis and DNA repair in different cell types including lung cancer. There are different p53 apoptotic pathways in high and low metastatic ability lung cancer cells. However, the exactly mechanism in the pathway is still unclear. Here we found that Annexin A2, a Ca2+ -dependent phospholipid-binding protein, is involved in p53-mediated apoptosis. First, by using mRNA differential display technique, down-regulated Annexin A2 expression was found in all cell lines transfected of Ad-p53 (adenoviral expression construct encoding wild type p53 gene) especially in highly metastatic Anip973 lung cancer cells. Then, decreased expression of Annexin A2 was further confirmed by Northern blot and Western blot analysis. At last, knock down of Annexin A2 by siRNA inhibited cellular proliferation in BE1 cell line with highly metastatic ability. Taken together, our results suggested that Annexin A2 may play roles in p53 induced apoptosis and it is also involved in regulation of cell proliferation.
Huang
Yun
Y
Laboratory of Medical Genetics, Harbin Medical University, No. 194, Xuefu road, Nangang district, Harbin, Heilongjing Province 150081, PR China.
Jin
Yan
Y
Yan
Cheng-Hui
CH
Yu
Yang
Y
Bai
Jing
J
Chen
Feng
F
Zhao
Yu-Zhen
YZ
Fu
Song-Bin
SB
D000256:Adenoviridae; D000257:Adenoviridae Infections; D017306:Annexin A2 / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D009362:Neoplasm Metastasis; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
C472822:ANXA2 protein, human; D017306:Annexin A2; D016159:Tumor Suppressor Protein p53