title: Teratogenic effects, acute and sub chronic toxicity of the leaf aqueous extract of Ocimum suave Wild (Lamiaceae) in rats.
abstract: The aqueous extract from the leaves of Ocimum suave was evaluated for acute and sub chronic toxicity and teratogenic effects. Swiss mice were administered single oral doses of 2000, 4000, 6000 and 8000 mg/kg and monitored for death and body weight gained for 7 days (acute toxicity). In sub-acute toxicity, experimental rats, received daily doses of 250, 500 and 1000 mg/kg for 42 consecutive days and the toxic effects were assessed using biochemical and haematological data and histology of vital organs. In a teratogenic study, 1-day pregnant rats were administered orally 500 and 1000 mg/kg of extract daily for 21 consecutive days and 14th day corpora lutea and foetal implants and litter size at birth were noted. Reproductive performance of F(1) generation rats was studied by crossing them at maturity and recording the number, birth weight and physical presentation of the young offspring. Acute intake of extract up to 8000 mg/kg did not produce mortality or significant changes in general behaviour. Sub chronic treatment did not show any change in body and organ weights, feeding habits or behaviour between the control and the treated groups of both sexes. Haematological analysis and blood biochemistry revealed no toxicity effects of the extract. No gross abnormalities or histological changes were observed. Teratogenic and fertility studies did not reveal any toxic manifestations or foetal abnormalities. The leaf aqueous extract of Ocimum suave is non toxic in acute and sub chronic intake.
authors.affiliation: Department of Animal Biology and Physiology, Faculty of Science, p.o. box 812, University of Yaoundé I, Cameroon. pvernyuy@yahoo.com
authors.forename: Paul V
authors.initials: PV
authors.lastname: Tan
authors.forename: Christophe
authors.initials: C
authors.lastname: Mezui
authors.forename: Georges
authors.initials: G
authors.lastname: Enow-Orock
authors.forename: Njifutie
authors.initials: N
authors.lastname: Njikam
authors.forename: Theophlie
authors.initials: T
authors.lastname: Dimo
authors.forename: Pauline
authors.initials: P
authors.lastname: Bitolog
mesh_terms: D000014:Abnormalities, Drug-Induced / Q000209:etiology*; D000284:Administration, Oral; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005247:Feeding Behavior / Q000187:drug effects; D005260:Female; D008098:Litter Size / Q000187:drug effects; D008297:Male; D051379:Mice; D031339:Ocimum / Q000737:chemistry*; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage / Q000633:toxicity*; D018515:Plant Leaves; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012098:Reproduction / Q000187:drug effects*; D018675:Toxicity Tests
chemical_list: D010936:Plant Extracts