Evaluation of Escherichia coli host strain CB390 for simultaneous detection of somatic and F-specific coliphages.
Escherichia coli WG5, the strain recommended by the International Organization for Standardization (ISO) to detect somatic coliphages, was transformed to F(+) by introducing the plasmid Famp, which rendered it capable of simultaneously detecting both somatic and F-specific coliphages. Indeed, this strain, CB390, proved as effective in detecting similar numbers of phages as the sum of somatic and F-specific bacteriophages detected by the host strains recommended by both the ISO and the U.S. Environmental Protection Agency standardized methods.
Guzmán
Carolina
C
Department of Microbiology, Biology Faculty, University of Barcelona, 645 Diagonal Avenue, 08028 Barcelona, Spain.
Mocé-Llivina
Laura
L
Lucena
Francisco
F
Jofre
Juan
J
D003090:Coliphages / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification; D004926:Escherichia coli / Q000821:virology*; D010948:Viral Plaque Assay