title: Interaction between RsgA and the ribosome.
abstract: RsgA is a unique GTP hydrolytic protein that is widely found in bacteria and plants, and is activated by the small subunit of the ribosome. Disruption of the gene for RsgA from the genome affects the growth of cells, the subunit association of the ribosome in cells and maturation of 16S ribosomal RNA. Here, we investigated the interaction between EscherichiacoliRsgA and the ribosome. Several antibiotics bound to the decoding center of the small subunit inhibited the ribosome-dependent GTPase activity of RsgA, suggesting that RsgA binds to the decoding center. Chemical footprinting was also performed to further investigate the interaction.
authors.affiliation: Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University.
authors.forename: Takatsugu
authors.initials: T
authors.lastname: Kimura
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Takagi
authors.forename: Hanawa-Suetsugu
authors.initials: HS
authors.lastname: Kyoko
authors.forename: Liliya
authors.initials: L
authors.lastname: Kalachnyuk
authors.forename: Akira
authors.initials: A
authors.lastname: Muto
authors.forename: Hyouta
authors.initials: H
authors.lastname: Himeno
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D029968:Escherichia coli Proteins / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D054680:Ribosome Subunits, Small, Bacterial / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D020558:GTP Phosphohydrolases; C467318:RsgA protein, E coli