Effects of electro-acupuncture on CNTF expression in spared dorsal root ganglion and the associated spinal lamina II and nucleus dorsalis following adjacent dorsal root ganglionectomies in cats.
It is well known that plasticity occurs in deafferented spinal cord, and that electro-acupuncture (EA) could promote functional restoration. The underlying mechanism is, however, unknown. Ciliary neurotrophic factor (CNTF) plays a crucial role in neurite outgrowth and neuronal survival both in vivo and in vitro, and its expression might explain some of the mechanism. In this study, we investigated the effects of EA on CNTF expression in the spared L(6) dorsal root ganglion (DRG), and spinal lamina II at spinal segments L(3) and L(6) as well as nucleus dorsalis (ND) of L(3) spinal segment following removal of L(1)-L(5) and L(7)-S(2) (DRG) in the cat. After ganglionectomies, the total and small-to-medium-sized numbers of immunoreactive neurons decreased at 3 dpo, and returned to the sham-operated level as early as 7 dpo. After EA, immunoreactive neurons in L(6) DRG noticeably increased at 7 dpo, compared with the non-acupunctured group. Notable increase in the large neurons was seen at 14 dpo, while their numbers in L(3) and L(6) spinal cord segments significantly declined at 3 dpo. Those in L(3) segment did not reach the sham-operated level until 14 dpo, but their numbers in L(6) segment returned to the sham-operated level as early as 7 dpo. CNTF immunopositive neurons in the ND of L(3) segment returned to the sham-operated level at 14 dpo. After EA, their number significantly increased as early as 7 dpo in lamina II of L(6) segment, and as late as 14 dpo in ND of L(3) segment. Western blot analysis showed CNTF changes corresponding to those shown in immunohistochemical staining. It is concluded that CNTF expression was involved in the EA promoted plastic changes in L(6) DRG and the associated deafferented spinal lamina and ND.
Zhou
Hao-Li
HL
Institute of Neurological Disease, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhang
Lian-Shuang
LS
Kang
Yan
Y
Zhang
Wei
W
Wang
Ting-Hua
TH
D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D002415:Cats; D002452:Cell Count; D020934:Ciliary Neurotrophic Factor / Q000096:biosynthesis*; D003714:Denervation; D015671:Electroacupuncture; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism* / Q000502:physiology*; D015171:Ganglionectomy; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*
D020934:Ciliary Neurotrophic Factor