Hemangiopoietin supports animal survival and accelerates hematopoietic recovery of chemotherapy-suppressed mice.
OBJECTIVE
To determine if recombinant human hemangiopoietin (HAPO), a novel growth factor for primitive cells of hematopoietic and endothelial cell lineages, accelerates hematopoietic reconstitution after high-dose chemotherapy in vivo in mice.


METHODS
Male Balb/c mice after treatment of 5-fluorouracil were subcutaneously injected with HAPO or its dilution for consecutive 10 d. Their survival and body weight together with peripheral blood were routinely tested. At day 7 and 14, the numbers of bone marrow (BM) cells as well as colony-forming units (CFU) after in vitro colony culture were counted. The peripheral blood CFU and the percentage of CD34+ CD117+ cells in BM were analyzed. Transwell chamber was used for cell migratory assay.


RESULTS
HAPO at different doses significantly increased the survival rate and body weight, with an optimal effect in the HAPO 10 microg/d group. The number of BM cells and the percentage of CD34+ CD117+ cells were also increased after HAPO administration. The number of granulocyte/macrophage CFU and granulocyte, erythroid, macrophage and megakaryocyte CFU in BM after HAPO treatment was greater than that from the HAPO dilution group. More circulating CFU could be observed after injection of HAPO. In addition, this novel cytokine had a chemotactic effect on the hematopoietic stem/progenitor cells.


CONCLUSIONS
HAPO improves animal survival and accelerates hematopoietic reconstitution of mice after high-dose chemotherapy.
Xu
Zi Liang
ZL
State Key Laboratory of Experimental Hematology, Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.
Zhou
Bin
B
Cong
Xiu Li
XL
Liu
Yong Jun
YJ
Xu
Bin
B
Li
Yan Han
YH
Gu
Jie
J
Han
Zhong Chao
ZC
D000818:Animals; D018952:Antigens, CD34 / Q000096:biosynthesis; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001835:Body Weight; D001854:Bone Marrow Cells / Q000378:metabolism; D002465:Cell Movement; D049109:Cell Proliferation; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011509:Proteoglycans / Q000502:physiology*; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis; D013234:Stem Cells; D000099158:Proteoglycan 4
D018952:Antigens, CD34; D000970:Antineoplastic Agents; D005472:Fluorouracil; D011509:Proteoglycans; D019009:Proto-Oncogene Proteins c-kit; C419197:PRG4 protein, human; D000099158:Proteoglycan 4