title: Capillary electrophoresis with capacitively coupled contactless conductivity detection for low molecular weight organic acids in different samples.
abstract: CE with capacitively coupled contactless conductivity detection (CE-C(4)D) was explored and validated for the identification and quantification of organic acids in various types of samples. The analyses were performed under optimized conditions, using a buffer system composed of 20 mM MES-histidine (His), pH 6.0, 0.1 mM CTAB, 0.025% HP-beta-CD, and 10% methanol. The investigation included a study of the effects of buffer pH, concentration of CTAB, type and concentration of organic additives, on the migration behavior, resolution and selectivity of the organic acids. The intra- and interday RSDs (n = 6) obtained for migration time and peak area were typically in the range of 0.12-2% and 0.5-4%, respectively. Linearity, detection limits, and repeatability were evaluated. In order to evaluate the application potential of the developed method, real samples from different sources were analyzed. The results demonstrate that CE-C(4)D is a versatile tool for analyzing organic acids in beverages, Chinese herbal medicines (CHM) and plants as it allows for their detection, identification, and quantification.
authors.affiliation: Department of Chemistry, National University of Singapore, Republic of Singapore.
authors.forename: Wai Siang
authors.initials: WS
authors.lastname: Law
authors.forename: Jian Hong
authors.initials: JH
authors.lastname: Zhao
authors.forename: Peter C
authors.initials: PC
authors.lastname: Hauser
authors.forename: Sam Fong Yau
authors.initials: SF
authors.lastname: Li
mesh_terms: D000143:Acids / Q000032:analysis* / Q000737:chemistry*; D000838:Anions / Q000737:chemistry; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D009930:Organic Chemicals / Q000032:analysis* / Q000737:chemistry*
chemical_list: D000143:Acids; D000838:Anions; D002593:Cetrimonium Compounds; D009930:Organic Chemicals; D000077286:Cetrimonium