RNA analysis with a novel fluorescent oligonucleotide.
We have designed and synthesized a novel fluorescent oligonucleotide to analyze functions of RNAs. This probe worked as a green fluorophore only when making a stable double-strand with DNA or RNA. The fluorescence enhancement is rapid, large and target specific. Therefore, this probe has an ideal photophysical property as a fluorescent probe. We applied a fluorescent oligonucleotide to living cells to confirm the condition for introducing the probe. This novel probe and analyzing method will be a very useful tool for understanding RNA functions.
Kubota
Takeshi
T
Frontier Reserarch System, RIKEN, 21 Hirosawa, Wako, Saitama 3510198, Japan.
Ikeda
Shuji
S
Okamoto
Akimitsu
A
D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D015345:Oligonucleotide Probes / Q000737:chemistry*; D012313:RNA / Q000032:analysis*; D013696:Temperature
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D012313:RNA