Investigation of antioxidant vitamins (A, E, C) and lipid peroxidation levels in rats injected N-(1,3-benzothiazol-2-yl)-N-(4,5-dihydro-1H-imidazol-2-yl) amine.
The present study examined the influence of synthetic N-(1,3-benzothiazol-2-yl)-N-(4,5-dihydro-1H-imidazol-2-yl) amine (2-Amdz) on levels of vitamins A, E and C and malondialdehyde (MDA) in rats. A total of 30 rats, divided into two groups, were used in the study. The control group was given only a subcutaneous injection of 250 microL 75% ethanol, every other day. The other group of rats was administered a subcutaneous injection of 2-Amdz (25 mg kg-1, dissolved in 250 microL of 75% ethanol). Injections were continued for 16 days. After the application of 2-Amdz for 16 days, the serum levels of vitamins A, E and C and malondialdehyde (MDA) were determined by HPLC. The serum vitamin A, E, and C levels decreased significantly compared to controls (p<0.05) whereas serum MDA levels were higher than control levels (p<0.005). As a result, it can be suggested that 2-Amdz induced a severe stress and more importantly, increased the amount of free radicals and significantly decreased the levels of serum antioxidant vitamins.
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Department of Chemistry, College of Science, Firat University, Elazig 23119, Turkey. fkaratas1@hotmail.com
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D000588:Amines / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000097:blood*; D052160:Benzothiazoles / Q000494:pharmacology*; D007093:Imidazoles / Q000494:pharmacology*; D007279:Injections, Subcutaneous; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D014801:Vitamin A / Q000097:blood*; D014810:Vitamin E / Q000097:blood*
D000588:Amines; D000975:Antioxidants; D052160:Benzothiazoles; D007093:Imidazoles; C525039:N-(1,3-benzothiazol-2-yl)-N-(4,5-dihydro-1H-imidazol-2-yl)amine; D014801:Vitamin A; D014810:Vitamin E; D008315:Malondialdehyde; D001205:Ascorbic Acid