title: Evaluation of microfluidics reactor technology on the kinetics of virus inactivation.
abstract: Mammalian cell lines constitute an important part in the manufacture of therapeutic proteins. However, their susceptibility to virus contamination is a potential risk to patient safety and productivity, and has led to the development of a repertoire of virus inactivation techniques. From a process development viewpoint, the challenge is to demonstrate the required log reduction in virus content without a significant loss in product titer or quality. The balance between the two is dictated by the kinetics of virus inactivation and protein degradation, both of which are critically affected by process parameters. In this study we describe a commercially available microchannel reactor (MCR) and demonstrate how it can be used to evaluate the impact of temperature on the kinetics of virus inactivation and protein product degradation. Virus spiking experiments are reported using Xenotropic Murine Leukemia Virus and REOvirus, into buffers in the absence and presence of a therapeutic protein currently under development at Lilly. The results demonstrate that the MCR is an ideal platform for evaluation of fast reactive systems and reactions that are particularly sensitive to small changes to process conditions. These conditions include heat inactivation of a virus in a mammalian cell culture process stream used in the manufacture of therapeutic proteins and antibodies.
authors.affiliation: Bioprocess Research and Development, Lilly Research Labs, Eli Lilly & Co, Indianapolis, Indiana, USA.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Bailey
authors.forename: Dayue
authors.initials: D
authors.lastname: Chen
authors.forename: Warren R
authors.initials: WR
authors.lastname: Emery
authors.forename: Peter K
authors.initials: PK
authors.lastname: Lambooy
authors.forename: Juliana
authors.initials: J
authors.lastname: Nolting
authors.forename: Michelle T
authors.initials: MT
authors.lastname: Quertinmont
authors.forename: Parviz A
authors.initials: PA
authors.lastname: Shamlou
mesh_terms: D019149:Bioreactors / Q000821:virology*; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006361:Heating / Q000295:instrumentation* / Q000379:methods; D007700:Kinetics; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D013242:Sterilization / Q000295:instrumentation* / Q000379:methods; D038301:Virus Inactivation