Investigating freshwater periphyton community response to uranium with phospholipid fatty acid and denaturing gradient gel electrophoresis analyses.
Periphyton communities can be used as monitors of ecosystem health and as indicators of contamination in lotic systems. Measures of biomass, community structure, and genetic diversity were used to investigate impacts of uranium (U) exposure on periphyton. Laboratory exposures of periphyton in river water amended with 238U were performed for 5 days, followed by 2 days of U depuration in unamended river water. Productivity as measured by biomass was not affected by concentrations up to 100 microg238U L(-1). Phospholipid fatty acid (PLFA) profiles and denaturing gradient gel electrophoresis (DGGE) banding patterns revealed no changes in community or genetic structure related to U exposure. We suggest that the periphyton community as a whole was not significantly impacted by exposures of 238U up to a concentration of 100 microgL(-1). These findings have significance for the assessment and prediction of U impacts on aquatic ecosystems.
Small
Jack A
JA
Pacific Northwest National Laboratory, Biotechnology and Ecotoxicology Group, P.O. Box 999, Mail Stop K2-21, 902 Battelle Boulevard, Richland, WA 99354, USA. Jack.Small@pnl.gov
Bunn
Amoret
A
McKinstry
Craig
C
Peacock
Aaron
A
Miracle
Ann L
AL
D018441:Biofilms; D018533:Biomass; D017753:Ecosystem; D004586:Electrophoresis; D005227:Fatty Acids / Q000378:metabolism*; D005618:Fresh Water; D019015:Geologic Sediments; D015233:Models, Statistical; D010743:Phospholipids / Q000032:analysis* / Q000737:chemistry; D045483:Rivers; D013997:Time Factors; D014501:Uranium / Q000032:analysis*; D014867:Water / Q000737:chemistry; D014872:Water Movements
D005227:Fatty Acids; D010743:Phospholipids; D014867:Water; D014501:Uranium