Integrated microfluidic cell culture and lysis on a chip.
We present an integrated microfluidic cell culture and lysis platform for automated cell analysis that improves on systems which require multiple reagents and manual procedures. Through the combination of previous technologies developed in our lab (namely, on-chip cell culture and electrochemical cell lysis) we have designed, fabricated, and characterized an integrated microfluidic platform capable of culturing HeLa, MCF-7, Jurkat, and CHO-K1 cells for up to five days and subsequently lysing the cells without the need to add lysing reagents. On-demand lysis was accomplished by local hydroxide ion generation within microfluidic chambers, releasing both proteinacious (GFP) and genetic (Hoescht-stained DNA) material. Sample proteins exposed to the electrochemical lysis conditions were immunodetectable (p53) and their enzymatic activity (HRP) was investigated.
Nevill
J Tanner
JT
Biomolecular Nanotechnology Center, Berkeley Sensor & Actuator Center, Department of Bioengineering, University of California, UCSF/UCB Joint Graduate Group in Bioengineering, Berkeley, CA, USA.
Cooper
Ryan
R
Dueck
Megan
M
Breslauer
David N
DN
Lee
Luke P
LP
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004563:Electrochemistry; D006801:Humans; D006878:Hydroxides / Q000737:chemistry / Q000378:metabolism; D007118:Immunoassay; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D016159:Tumor Suppressor Protein p53 / Q000276:immunology
D006878:Hydroxides; D016159:Tumor Suppressor Protein p53; C031356:hydroxide ion