title: A serine involved in actin-dependent subcellular localization of a stress-induced tobacco BY-2 hydroxymethylglutaryl-CoA reductase isoform.
abstract: 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGR) is unique in the first part of the cytoplasmic isoprenoid pathway, as it contains a membrane domain that includes ER-specific retention motifs. When fused to GFP, this domain targets two tobacco BY-2 HMGR isoforms differentially. While the first isoform is ER-localized, a second stress-induced one forms globular structures connected by tubular structures. A serine positioned upstream of the ER retention motif seems to be implicated in this specific subcellular localization. Surprisingly, these structures are closely connected to F-actin, and their intactness is dependent upon the integrity of the filaments or the action of a calmodulin antagonist.
authors.affiliation: Institut de Biologie Moléculaire des Plantes CNRS-UPR 2357, Université Louis Pasteur, Département Isoprénoïdes, 28 Rue Goethe, F-67083 Strasbourg, France.
authors.forename: Rémy
authors.initials: R
authors.lastname: Merret
authors.forename: Jean- Roger
authors.initials: J
authors.lastname: Cirioni
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Bach
authors.forename: Andréa
authors.initials: A
authors.lastname: Hemmerlin
mesh_terms: D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D004721:Endoplasmic Reticulum / Q000201:enzymology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006903:Hydroxymethylglutaryl CoA Reductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000737:chemistry; D013347:Subcellular Fractions / Q000201:enzymology; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics
chemical_list: D000199:Actins; D018744:DNA, Plant; D007527:Isoenzymes; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D012694:Serine; D006903:Hydroxymethylglutaryl CoA Reductases