Rapid validated method for the analysis of benzo[a]pyrene in vegetable oils by using solid-phase microextraction-gas chromatography-mass spectrometry.
A solid-phase microextraction method coupled with comprehensive gas chromatography and time-of-flight mass spectrometry for the determination of polycyclic aromatic hydrocarbons in vegetable oils has recently been reported. The present paper tested the possibility to use the solid-phase microextraction method coupled with one-dimensional gas chromatography-mass spectrometry of the only benzo[a]pyrene. Furthermore, an in-house validation for benzo[a]pyrene, used as a marker, as requested by the European regulation no. 208/2005, was carried out. Statistical tests were performed to elaborate the data. Linearity was satisfactory (r(2)=0.999), between about 0.5 and 15 microg/kg. Detection limit and quantification limit were 0.17 and 0.46 microg/kg, respectively. In-day and inter-day repeatability were less than 6% in both cases.
Purcaro
Giorgia
G
Department of Food Science, University of Udine, via Marangoni 97, 33100 Udine, Italy. giopurcaro@libero.it
Moret
Sabrina
S
Conte
Lanfranco S
LS
D001564:Benzo(a)pyrene / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010938:Plant Oils / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D010938:Plant Oils; D001564:Benzo(a)pyrene