Development and validation of high-throughput liquid chromatography-tandem mass spectrometric method for simultaneous quantification of loratadine and desloratadine in human plasma.
As a continuation of effort to improve our high flow on-line bioanalytical approach for high-throughput quantification of drugs and metabolites in plasma by high-throughput liquid chromatography tandem mass spectrometry (HTLC-MS/MS), we have developed a simple, sensitive and reliable method for simultaneous quantification of loratadine and desloratadine in human plasma. We have performed on-line coupling of extraction with Cyclone P 50 mm x 0.5 mm 50 microm HTLC column and chromatographic separation is performed with Zorbax XDB C18 50 mm x 2.1 mm 5 microm, followed by quantification with mass detector. The method is validated and showed good performances in terms of linearity, sensitivity, precision, accuracy and stability. A marked improvement in sample throughput efficiency is realized with this method and the proposed method will be useful for pharmacokinetic and/or bioequivalence studies.
Srinubabu
G
G
Clinical Research and Biosciences (I) Pvt. Ltd., Tarnaka, Hyderabad 500017, India. srinubabuau6@gmail.com
Patel
Rajaram S
RS
Shedbalkar
Vinay P
VP
Rao
Allam Appa
AA
Rao
M Narasimha
MN
Bandaru
Veera Venkata Ratnam
VV
D002853:Chromatography, Liquid / Q000379:methods*; D039563:Histamine H1 Antagonists, Non-Sedating / Q000097:blood*; D006801:Humans; D017336:Loratadine / Q000031:analogs & derivatives* / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D039563:Histamine H1 Antagonists, Non-Sedating; D017336:Loratadine; C121345:desloratadine