title: Resistance of the Manila clam (Venerupis philippinarum) to infection with Mikrocytos mackini.
abstract: Manila clams (Venerupis philippinarum) challenged in laboratory trials via bath exposure proved to be resistant to infections with Mikrocytos mackini (protistan parasite of unknown taxonomic affiliation), while Pacific oysters (Crassostrea gigas) challenged simultaneously using identical conditions developed infections. Although M. mackini was detected by a nucleic acid pathogen specific (PCR) assay in 10-30% of the challenged V. philippinarum that were sampled soon after exposure (0-48 h, n=40), all of the subsequent V. philippinarum (n=62) sampled 9-17 weeks post-exposure tested negative for M. mackini by PCR assay. Prevalence of infection for the exposed C. gigas (n=100) during this same period ranged from 50% to 100% by PCR assay. Infection was confirmed in the oysters (58%, n=60) by a digoxigenin-labelled DNA probe designed to detect M. mackini by in situ hybridization, but M. mackini was not found in any of the exposed Manila clams (n=63) using this technique.
authors.affiliation: Fisheries and Oceans Canada, Pacific Biological Station, 3190 Hammond Bay Road, Nanaimo, BC, Canada V9T 6N7. meyerg@dfo-mpo.gc.ca
authors.forename: Gary R
authors.initials: GR
authors.lastname: Meyer
authors.forename: Susan M
authors.initials: SM
authors.lastname: Bower
authors.forename: Geoff
authors.initials: G
authors.lastname: Lowe
authors.forename: Sarah
authors.initials: S
authors.lastname: Davies
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000469:parasitology* / Q000502:physiology; D016054:DNA, Protozoan / Q000032:analysis; D004198:Disease Susceptibility / Q000469:parasitology*; D005057:Eukaryotic Cells / Q000502:physiology*; D007113:Immunity, Innate / Q000502:physiology; D010125:Ostreidae / Q000469:parasitology / Q000502:physiology; D011529:Protozoan Infections, Animal / Q000469:parasitology; D013997:Time Factors
chemical_list: D016054:DNA, Protozoan