Amino acid pool composition of the basidiomycete Coprinus cinereus.
The leaf-litter fungus Coprinus cinereus maintains a pool of free amino acid in its mycelium. When the organism is grown under conditions of high nitrogen availability with 13.2 mmol.L-1 L-asparagine as the nitrogen source, the primary constituents of this pool are glutamine, alanine, and glutamic acid. Together these 3 amino acids comprise approximately 70% of the pool. Nitrogen deprivation reduces the size of the free amino acid pool by 75%, and neither a high concentration of ammonium nor a protein nitrogen source support a similar pool size as L-asparagine. Nitrogen deprivation also reduces the concentration of glutamine to the pool while increasing glutamate. Concomitant with this shift is a marked increase in mycelial ammonium.
Ulrich
Cynthia E
CE
Department of Biology, Southeast Missouri State University, Cape Girardeau, MO 63701, USA.
Gathman
Allen C
AC
Lilly
Walt W
WW
D000596:Amino Acids / Q000032:analysis*; D001216:Asparagine / Q000032:analysis; D003302:Coprinus / Q000737:chemistry* / Q000378:metabolism; D025282:Mycelium / Q000737:chemistry*; D009584:Nitrogen / Q000032:analysis
D000596:Amino Acids; D001216:Asparagine; D009584:Nitrogen