Rapid determination of hazardous compounds in food based on a competitive fluorescence microsphere immunoassay.
Development of a microsphere-based competitive fluorescence immunoassay for the determination of hazardous low-molecular-weight compounds in food is described. In this method, antigens are covalently bound to carboxy-modified microspheres to compete monoclonal antibody with low-molecular-weight compounds in food samples; mouse IgG/fluorescein isothiocyanate conjugate is used as the fluorescent molecular probe. Thus, the hazardous low-molecular-weight compounds are quantified using a multiparameter flow cytometer. This method has been evaluated using clenbuterol as a model compound. It has a sensitivity of 0.01 ng/mL with dynamic range of 0.01-100 ng/mL, and the concentration of clenbuterol providing 50% inhibition (IC(50)) is 1.1 ng/mL. The main advantages of this method are its high efficiency, biocompatibility, and selectivity, as well as ultralow trace sample consumption and low cost.
Zou
Mingqiang
M
Chinese Academy of Inspection and Quarantine, 100025 Beijing, China. mingqiangz@sina.com
Gao
Haixia
H
Li
Jinfeng
J
Xu
Fang
F
Wang
Ling
L
Jiang
Jizhi
J
D001667:Binding, Competitive; D002976:Clenbuterol / Q000032:analysis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005453:Fluorescence; D005504:Food Analysis / Q000379:methods*; D005513:Food Inspection / Q000379:methods; D015386:Hazardous Substances / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D008863:Microspheres; D008970:Molecular Weight; D013997:Time Factors
D015386:Hazardous Substances; D002976:Clenbuterol