Validation and uncertainty analysis of a multi-residue method for  pesticides in grapes using ethyl acetate extraction and liquid  chromatography-tandem mass spectrometry.
A method was validated for the multi-residue analysis of 82 pesticides in grapes at <or=25 ng/g level. Berry samples (10 g) mixed with sodium sulphate (10 g) were extracted with ethyl acetate (10 mL); cleaned by dispersive solid phase extraction and the results were obtained by liquid chromatography-tandem mass spectrometry. Reduction in sample size and proportion of ethyl acetate for extraction did not affect accuracy or precision of analysis when compared to the reported methods and was also statistically similar to the QuEChERS technique. The method was rugged (HorRat < 0.5) with <20% measurement uncertainties. Limit of quantification was <10 ng/g with recoveries 70-120% for most pesticides. The method offers cheaper and safer alternative to typical multi-residue analysis methods for grape.
Banerjee
Kaushik
K
National Research Centre for Grapes, P.O. Manjri Farm, Pune 412 307, India. kbgrape@yahoo.com
Oulkar
Dasharath P
DP
Dasgupta
Soma
S
Patil
Shubhangi B
SB
Patil
Sangram H
SH
Savant
Rahul
R
Adsule
Pandurang G
PG
D000085:Acetates / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D010575:Pesticides / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D027843:Vitis / Q000737:chemistry*
D000085:Acetates; D010575:Pesticides; C007650:ethyl acetate