Uniform and prolonged changes in blood oxidative stress after muscle-damaging exercise.
BACKGROUND
The effect of eccentric exercise on the time-course changes in several indices of muscle damage and blood oxidative stress was examined.


METHODS
Isometric torque, delayed-onset muscle soreness, creatine kinase, reduced glutathione (GSH), oxidized glutathione (GSSG), thiobarbituric-acid reactive substances (TBARS), protein carbonyls, catalase, uric acid, bilirubin and total antioxidant capacity (TAC) in blood were measured pre-, 24 h, 48 h and 72 h post-eccentric exercise of knee extensors in ten females.


RESULTS
The concentration of all oxidative stress indices changed significantly in a way indicating increased oxidative stress in the blood (GSH and GSH/GSSG, decreased, whereas GSSG, TBARS, protein carbonyls, catalase, uric acid, bilirubin and TAC increased) peaking, in all but TBARS, at 48 h and returning toward baseline afterwards.


CONCLUSIONS
We believe that muscle-damaging exercise should be viewed as a different challenge compared to non-muscle-damaging exercise with regard to its effects on blood oxidative stress.
Paschalis
Vassilis
V
Department of Physical Education and Sport Science, University of Thessaly, 42100, Trikala, Greece.
Nikolaidis
Michalis G
MG
Fatouros
Ioannis G
IG
Giakas
Giannis
G
Koutedakis
Yiannis
Y
Karatzaferi
Christina
C
Kouretas
Dimitris
D
Jamurtas
Athanasios Z
AZ
D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D001663:Bilirubin / Q000097:blood; D002374:Catalase / Q000378:metabolism; D004958:Estradiol / Q000097:blood; D015444:Exercise / Q000502:physiology*; D005260:Female; D005978:Glutathione / Q000097:blood; D006801:Humans; D009132:Muscles / Q000473:pathology / Q000503:physiopathology*; D018384:Oxidative Stress; D050050:Protein Carbonylation; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014527:Uric Acid / Q000097:blood
D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D014527:Uric Acid; D004958:Estradiol; D002374:Catalase; D005978:Glutathione; D001663:Bilirubin