title: Clean-up method of forchlorfenuron in agricultural products for HPLC analysis.
abstract: A simplified method for the determination of forchlorfenuron in agricultural products by HPLC with UV detection was investigated. A chopped sample homogenate from agricultural products was extracted with acetone. The extract was filtrated and concentrated. The residues was loaded onto a Chem Elut column and extracted with ethyl acetate. The crude extract was purified on Oasis HLB and Bond Elut PSA mini-columns using a mixture of methanol and ethyl acetate. Forchlorfenuron was analyzed by HPLC with UV detection (263 nm). HPLC separation was performed on an ODS column with methanol-water as the mobile phase. Recoveries of forchlorfenuron from several agricultural products fortified at the level of 0.1 microg/g were in the range of 87.6-99.5%. The limit of detection (S/N=3) was 0.005 microg/g in the sample.
authors.affiliation: Tokyo Metropolitan Institute of Public Health, 3-24-1 Hyakunincho, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takano
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tamura
authors.forename: Sanae
authors.initials: S
authors.lastname: Tomizawa
authors.forename: Yukinari
authors.initials: Y
authors.lastname: Tateishi
authors.forename: Naoko
authors.initials: N
authors.lastname: Sakai
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kamijo
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ibe
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nagayama
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D018556:Crops, Agricultural / Q000737:chemistry*; D010671:Phenylurea Compounds / Q000032:analysis*; D010937:Plant Growth Regulators / Q000032:analysis*; D011725:Pyridines / Q000032:analysis*
chemical_list: D010671:Phenylurea Compounds; D010937:Plant Growth Regulators; D011725:Pyridines; C116093:N-(2-chloro-4-pyridyl)-N'-phenylurea