Regulation of flowering time by the protein arginine methyltransferase AtPRMT10.
In plants, histone H3 lysine methyltransferases are important in gene silencing and developmental regulation; however, the roles of histone H4 methylation in plant development remain unclear. Recent studies found a type II histone arginine methyltransferase, AtPRTM5, which is involved in promoting growth and flowering. Here, we purified a dimerized plant-specific histone H4 methyltransferase, plant histone arginine methyltransferase 10 (PHRMT10), from cauliflower. Arabidopsis thaliana protein arginine methyltransferase 10 (AtPRMT10)--the Arabidopsis homologue of PHRMT10--was shown to be a type I PRMT, which preferentially asymmetrically methylated histone H4R3 in vitro. Genetic disruption of AtPRMT10 resulted in late flowering by upregulating FLOWERING LOCUS C (FLC) transcript levels. In addition, we show that AtPRMT10 functions genetically separate from AtPRMT5, but that each acts to fine-tune expression of FLC. This work adds an extra layer of complexity to flowering-time regulation and also sheds light on the importance of asymmetric arginine methylation in plant development.
Niu
Lifang
L
State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.
Lu
Falong
F
Pei
Yanxi
Y
Liu
Chunyan
C
Cao
Xiaofeng
X
D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001937:Brassica / Q000201:enzymology / Q000235:genetics; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D035264:Flowers / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005816:Genetic Complementation Test; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000378:metabolism; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011496:Protein Methyltransferases; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
D029681:Arabidopsis Proteins; D010940:Plant Proteins; D000076983:Histone Methyltransferases; D008780:Methyltransferases; C529430:PRMT10 protein, Arabidopsis; D011496:Protein Methyltransferases; D011484:Protein-Arginine N-Methyltransferases; D011495:Histone-Lysine N-Methyltransferase