Selective oxidation of methylcytosine in single base pair mismatches by osmium complex.
We have reported a novel method for methylcytosine (M)-positive epigenotyping, in which an osmium ion site-specifically binds to M in a single strand or a bulge structure of DNA on oxidation of an M C5-C6 double bond. Herein, we have examined the osmium oxidation reactivity of M that formed a mismatched base pair with various bases, and observed its unique reactivity.
Nomura
Akiko
A
Frontier Research System, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Tainaka
Kazuki
K
Okamoto
Akimitsu
A
D044503:5-Methylcytosine / Q000032:analysis* / Q000737:chemistry; D020137:Base Pair Mismatch; D004591:Electrophoresis, Polyacrylamide Gel; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D009992:Osmium / Q000737:chemistry*; D010084:Oxidation-Reduction
D009838:Oligodeoxyribonucleotides; D009992:Osmium; D044503:5-Methylcytosine