title: Good manufacturing practice-compliant expansion of marrow-derived stem and progenitor cells for cell therapy.
abstract: Ex vivo expansion is being used to increase the number of stem and progenitor cells for autologous cell therapy. Initiation of pivotal clinical trials testing the efficacy of these cells for tissue repair has been hampered by the challenge of assuring safe and high-quality cell production. A strategy is described here for clinical-scale expansion of bone marrow (BM)-derived stem cells within a mixed cell population in a completely closed process from cell collection through postculture processing using sterile connectable devices. Human BM mononuclear cells (BMMNC) were isolated, cultured for 12 days, and washed postharvest using either standard open procedures in laminar flow hoods or using automated closed systems. Conditions for these studies were similar to long-term BM cultures in which hematopoietic and stromal components are cultured together. Expansion of marrow-derived stem and progenitor cells was then assessed. Cell yield, number of colony forming units (CFU), phenotype, stability, and multilineage differentiation capacity were compared from the single pass perfusion bioreactor and standard flask cultures. Purification of BMMNC using a closed Ficoll gradient process led to depletion of 98% erythrocytes and 87% granulocytes, compared to 100% and 70%, respectively, for manual processing. After closed system culture, mesenchymal progenitors, measured as CD105+CD166+CD14-CD45- and fibroblastic CFU, expanded 317- and 364-fold, respectively, while CD34+ hematopoietic progenitors were depleted 10-fold compared to starting BMMNC. Cultured cells exhibited multilineage differentiation by displaying adipogenic, osteogenic, and endothelial characteristics in vitro. No significant difference was observed between manual and bioreactor cultures. Automated culture and washing of the cell product resulted in 181 x 10(6) total cells that were viable and contained fibroblastic CFU for at least 24 h of storage. A combination of closed, automated technologies enabled production of good manufacturing practice (GMP)-compliant cell therapeutics, ready for use within a clinical setting, with minimal risk of microbial contamination.
authors.affiliation: Institut für Laboratoriums- und Transfusionsmedizin, Herz- und Diabeteszentrum Nordrhein-Westfalen, Universitätsklinik der Ruhr-Universität Bochum, 32545 Bad Oeynhausen, Germany. gastens@gmx.de
authors.forename: Martin H
authors.initials: MH
authors.lastname: Gastens
authors.forename: Kristin
authors.initials: K
authors.lastname: Goltry
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Prohaska
authors.forename: Diethelm
authors.initials: D
authors.lastname: Tschöpe
authors.forename: Bernd
authors.initials: B
authors.lastname: Stratmann
authors.forename: Dirk
authors.initials: D
authors.lastname: Lammers
authors.forename: Stanley
authors.initials: S
authors.lastname: Kirana
authors.forename: Christian
authors.initials: C
authors.lastname: Götting
authors.forename: Knut
authors.initials: K
authors.lastname: Kleesiek
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D018929:Cell Culture Techniques / Q000295:instrumentation; D019070:Cell Lineage; D064987:Cell- and Tissue-Based Therapy / Q000295:instrumentation / Q000379:methods / Q000592:standards; D003114:Colony-Forming Units Assay; D019983:Guideline Adherence; D006801:Humans; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*