Genotoxicity evaluation of the insecticide endosulfan in the wetland macrophyte Bidens laevis L.
The frequency of micronuclei (MN) and chromosome aberrations in anaphase-telophase (CAAT) was determined in root tips of the wetland macrophyte Bidens laevis exposed to environmentally relevant concentrations of endosulfan (0.01, 0.02, 0.5 and 5microg/L) for 48h. MN frequency varied from 0 in negative controls and plants exposed to 0.01microg/L endosulfan to 0-3 in plants exposed to 5microg/L. Moreover, a significant concentration-dependent increase of CAAT was observed. The higher proportion of laggards and vagrand chromosomes observed at 5microg/L would indicate that endosulfan interacts with the spindle interrupting normal chromosome migration. Endosulfan resulted genotoxic to B. laevis, a species of potential value for bioassays and in situ monitoring of environmental contamination by pesticides.
Pérez
Débora J
DJ
Laboratorio de Genética, Estación Experimental Agropecuaria Balcarce INTA - Facultad de Ciencias Agrarias - UNMdP, CC 276, 7620 Balcarce, Argentina.
Menone
Mirta L
ML
Camadro
Elsa L
EL
Moreno
Víctor J
VJ
D001118:Argentina; D031176:Bidens / Q000187:drug effects* / Q000235:genetics; D004249:DNA Damage; D004726:Endosulfan / Q000633:toxicity*; D004784:Environmental Monitoring / Q000379:methods; D004785:Environmental Pollutants / Q000633:toxicity*; D017343:Genes, Plant / Q000187:drug effects*; D007306:Insecticides / Q000633:toxicity*; D015162:Micronucleus Tests; D009152:Mutagenicity Tests / Q000379:methods; D053833:Wetlands
D004785:Environmental Pollutants; D007306:Insecticides; D004726:Endosulfan