title: Influences of adhesion area and biological sample size on the estimation of Young's modulus and Poisson's ratio assessed by micropipette aspiration technique.
abstract: The micropipette aspiration experiment remains a widely used micromanipulation technique for quantifying the mechanical properties of biological samples. Our study extends previous results by investigating the influence of sample size and adhesion area on the mechanical response of compressible thin biological samples. We thus defined a nonlinear relationship between aspirated length, Young's modulus, Poisson's ratio and sample thickness which allowed us to develop an original experimental protocol for simultaneous quantification of the Poisson's ratio and Young's modulus of adherent samples. We first validated our method by characterizing mechanical properties of polyacrylamide gels with tunable stiffness. We then considered application of these results to the quantification of cell elasticity, focusing on the influence of cell adhesion area onto the measured apparent cell stiffness.
authors.affiliation: Laboratoire TIMC-IMAG, Dynacell, Centre National de la Recherche Scientifique, UMR 5525, IN3S, 38706 La Tronche Cedex, France. Thomas.Boudou@imag.fr
authors.forename: T
authors.initials: T
authors.lastname: Boudou
authors.forename: J
authors.initials: J
authors.lastname: Ohayon
authors.forename: C
authors.initials: C
authors.lastname: Picart
authors.forename: P
authors.initials: P
authors.lastname: Tracqui
mesh_terms: D000268:Adhesiveness; D016880:Anisotropy; D016477:Artifacts; D002448:Cell Adhesion / Q000502:physiology*; D002468:Cell Physiological Phenomena; D003198:Computer Simulation; D003238:Connective Tissue / Q000502:physiology*; D004548:Elasticity; D008954:Models, Biological; D010812:Physical Stimulation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013314:Stress, Mechanical; D013396:Suction / Q000379:methods*