Probing the mutation spectrum in E. coli.
The mutation spectrum, together with mutation frequency, is decisive for the size and nature of genetic diversity. We constructed plasmid-encoded probes for specific detection of each and all of the six base substitutions. Using the set of the probes, we analyzed the mutation spectrumon the plasmids caused by different types of mutagens and mutator enzymes/alleles.
Suzuki
Tsuyoshi
T
Gaduate School of Engineering, Chiba Univeristy, 1-33, Yayoi-cho, Inage-ku, Chiba City, Chiba 263-8522, Japan.
Suzuki
Keiichi
K
Tashiro
Yohei
Y
Saito
Kyoichi
K
Umeno
Daisuke
D
D004252:DNA Mutational Analysis / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D016296:Mutagenesis; D010957:Plasmids / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics
D001618:beta-Lactamases