D-HPLC analysis of the entire FLT3 gene in MLL rearranged and hyperdiploid acute lymphoblastic leukemia.
MLL rearranged and hyperdiploid acute lymphoblastic leukemia (ALL) are characterized by high-level FLT3 expression and constitutive FLT3 activation. As known activating FLT3 mutations are often absent in these patients, we screened the entire FLT3 coding sequence in MLL rearranged and hyperdiploid ALL cases for yet unidentified additional genetic alterations using denaturing D-HPLC. Both in MLL rearranged and hyperdiploid ALL we found that a small minority of samples, 7% and 10% respectively, carried genetic alterations. Although some of these alterations may induce FLT3 activation, the majority of these patients carry wild-type FLT3 genes.
Stam
Ronald W
RW
Erasmus MC/Sophia Children's Hospital, Department of Pediatric Oncology/Hematology, Room Sp 2456, Dr. Molewaterplein 60, P.O. Box 2060, 3000 CB Rotterdam, The Netherlands.
den Boer
Monique L
ML
Schneider
Pauline
P
Meier
Marrit
M
Beverloo
H Berna
HB
Pieters
Rob
R
D002851:Chromatography, High Pressure Liquid; D004252:DNA Mutational Analysis; D015321:Gene Rearrangement; D006801:Humans; D051788:Myeloid-Lymphoid Leukemia Protein / Q000235:genetics*; D011003:Ploidies; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000453:epidemiology / Q000235:genetics*; D051941:fms-Like Tyrosine Kinase 3 / Q000235:genetics*
D051788:Myeloid-Lymphoid Leukemia Protein; D051941:fms-Like Tyrosine Kinase 3