title: Electrochemical biosensors based on DNA strand exchange.
abstract: Electrochemical biosensors for the detection of specific DNA sequences have been described. The redox-modified DNA sequences were hybridized with the 3'-thiol-modified DNA sequences as capture DNA strand. The resulting duplexes were assembled on a gold electrode surface to make DNA chip. Upon addition of fully complementary DNA or mismatch-containing DNA, the time-dependent changes in redox signal were monitored. Mismatched DNA gave less than 20% change when compared with fully matched DNA under the same conditions. The present assay format is thus applicable in discrimination of mutated DNA from fully matched DNA.
authors.affiliation: Department of Materials Science and Chemistry, University of Hyogo, 2167 Shosha, Himeji 671-2201, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Watanabe
authors.forename: Jyun
authors.initials: J
authors.lastname: Yoshizumi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kumamoto
authors.forename: Mitsunobu
authors.initials: M
authors.lastname: Nakamura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Maruyama
authors.forename: Kazushige
authors.initials: K
authors.lastname: Yamana
mesh_terms: D000880:Anthraquinones / Q000737:chemistry; D020137:Base Pair Mismatch; D015374:Biosensing Techniques; D004252:DNA Mutational Analysis / Q000379:methods*; D004563:Electrochemistry; D015345:Oligonucleotide Probes / Q000737:chemistry; D010084:Oxidation-Reduction
chemical_list: D000880:Anthraquinones; D015345:Oligonucleotide Probes