Polymerisation of a DNA strand using oligo-DNA template with modified bases, sugars and phosphates.
We have attempted to synthesise a complimentary strand using oligo-DNA with modified bases, sugars and phosphates as a template strand by polymerase reaction. Analogues bearing C5-substituted uracil, those with amide linkage [-CH2C=ONH-] in place of phospho-diester linkage and those bearing 2'-O, 4'-C-bridged sugar were used. Primer extension reactions were carried out to synthesise complimentary DNA strands. The reactions depended on the thermostable DNA polymerase used, the type of modification or the number of the modified position on the template strand.
Nagashima
Jun-Ichi
J
Graduate School of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan.
Minezaki
Satoshi
S
Obika
Satoshi
S
Imanishi
Takeshi
T
Kuwahara
Masayasu
M
Sawai
Hiroaki
H
D000577:Amides / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry; D004247:DNA / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D013698:Templates, Genetic; D014498:Uracil / Q000031:analogs & derivatives
D000577:Amides; D002241:Carbohydrates; D009838:Oligodeoxyribonucleotides; D010710:Phosphates; D014498:Uracil; D004247:DNA; D004259:DNA-Directed DNA Polymerase