Physically enhanced coating of a titanium plasma-spray surface with human SAOS-2 osteoblasts and extracellular matrix.
Several studies suggest that the modification of a titanium surface could play an important role in bone tissue engineering. In this study we have followed a biomimetic strategy where ultrasonically or electromagnetically stimulated human SAOS-2 osteoblasts proliferated and built their extracellular matrix on a titanium plasma-spray surface. In comparison with control conditions, the ultrasonic stimulus (power, 149 mW; frequency, 1.5 MHz) and the electromagnetic stimulus (magnetic field, 2 mT; frequency, 75 Hz) increased the cell proliferation and the surface coating with type-I collagen. The physical stimuli aimed at obtaining a better modification of the rough titanium plasma-spray surface in terms of cell colonization and coating with bone matrix. The superficially modified biomaterial could be used, in clinical applications, as an implant for bone repair.
Fassina
Lorenzo
L
University of Pavia, Dipartimento di Informatica e Sistemistica, Centro di Ingegneria Tissutale (C.I.T.), via Ferrata 1, 27100 Pavia, Italy. lorenzo.fassina@unipv.it
Saino
Enrica
E
Visai
Livia
L
Magenes
Giovanni
G
D001861:Bone Regeneration; D018786:Bone Substitutes; D045744:Cell Line, Tumor; D024042:Collagen Type I / Q000378:metabolism; D004247:DNA / Q000032:analysis; D055590:Electromagnetic Phenomena; D005109:Extracellular Matrix; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014465:Ultrasonics
D018786:Bone Substitutes; D024042:Collagen Type I; D004247:DNA; D014025:Titanium