DNA digestion to deoxyribonucleoside: a simplified one-step procedure.
We present a simple and inexpensive 'one-step' protocol for the hydrolysis of DNA to deoxyribonucleosides. Unlike the older DNA hydrolysis protocol which is cumbersome and labor intensive, this new protocol is ideal for high-throughput assays and is suitable automation. Using this protocol we were able to hydrolyze several hundred samples within an 8-hour period. The new protocol is fully compatible with LC-MS/MS and gives similar recoveries for all five major deoxyribonucleosides when compared to the older protocol.
Quinlivan
Eoin P
EP
Biomedical Mass Spectrometry Laboratory, General Clinical Research Center, University of Florida, Gainesville, FL 32611, USA. epq@ufl.edu
Gregory
Jesse F
JF
D004247:DNA / Q000378:metabolism*; D003853:Deoxyribonucleosides / Q000096:biosynthesis*; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D004722:Endoribonucleases / Q000378:metabolism*; D006868:Hydrolysis
D003853:Deoxyribonucleosides; D004247:DNA; D004706:Endodeoxyribonucleases; D004722:Endoribonucleases; C021835:Serratia marcescens nuclease