Size-exclusion chromatography based on silica-diol for the analysis of the proinsulin fusion protein.
Size-exclusion chromatography based on silica-diol sorbent was employed to analyze the recombinant proinsulin fusion protein obtained during the process of refolding and the following ion-exchange purification. The assay was qualified as a control method estimating its accuracy, precision, linearity, limit of detection, limits of quantitation, specificity, and robustness. The results show the reliability for the intended use.
Gusarova
V
V
Experimental Biotechnological Plant, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of Russian Academy of Sciences, Mikloukho-Maklaya Street, 16/10, Moscow 117997, Russian Federation. gusarovavd@list.ru
Vorobjeva
T
T
Gusarov
D
D
Lasman
V
V
Bayramashvili
D
D
D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D011384:Proinsulin / Q000032:analysis*; D011993:Recombinant Fusion Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D011993:Recombinant Fusion Proteins; D011384:Proinsulin