title: Crystallization and preliminary crystallographic analysis of an octaketide-producing plant type III polyketide synthase.
abstract: Octaketide synthase (OKS) from Aloe arborescens is a plant-specific type III polyketide synthase that produces SEK4 and SEK4b from eight molecules of malonyl-CoA. Recombinant OKS expressed in Escherichia coli was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group I422, with unit-cell parameters a = b = 110.2, c = 281.4 A, alpha = beta = gamma = 90.0 degrees . Diffraction data were collected to 2.6 A resolution using synchrotron radiation at BL24XU of SPring-8.
authors.affiliation: Mitsubishi Kagaku Institute of Life Sciences (MITILS), 11 Minamiooya, Machida, Tokyo 194-8511, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Morita
authors.forename: Shin
authors.initials: S
authors.lastname: Kondo
authors.forename: Ryohei
authors.initials: R
authors.lastname: Kato
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Wanibuchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Noguchi
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Sugio
authors.forename: Ikuro
authors.initials: I
authors.lastname: Abe
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kohno
mesh_terms: D000504:Aloe / Q000201:enzymology; D003460:Crystallization; D018360:Crystallography, X-Ray; D048630:Polyketide Synthases / Q000737:chemistry*
chemical_list: D048630:Polyketide Synthases