Differential two-signal picosecond-pulse coherent anti-Stokes Raman scattering imaging microscopy by using a dual-mode optical parametric oscillator.
We propose and demonstrate a novel differential two-signal technique of coherent anti-Stokes Raman scattering (CARS) imaging microscopy using a picosecond (ps) optical parametric oscillator (OPO). By adjusting a Lyot filter inside the cavity, we operated the OPO oscillating in two stable modes separated by a few nanometers. The CARS images generated by the two modes are separated by a spectrograph behind the microscope setup, and their differential image is directly obtained by balanced lock-in detection. The feasibility of the technique is experimentally verified by imaging micrometer-sized polystyrene beads immersed in water.
Yoo
Yong Shim
YS
Division of Physical Metrology, Korea Research Institute of Standards and Science, Doryong-dong 1, Yuseong-gu, Daejeon, 305-600, Korea. ysyoo@kriss.re.kr
Lee
Dong-Hoon
DH
Cho
Hyuck
H
D000465:Algorithms; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods; D055095:Optics and Photonics; D009991:Oscillometry / Q000295:instrumentation* / Q000379:methods; D011137:Polystyrenes / Q000737:chemistry; D012542:Scattering, Radiation; D013053:Spectrophotometry / Q000379:methods; D013059:Spectrum Analysis, Raman; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods
D011137:Polystyrenes