[Multi-residue method for determination of veterinary drugs and feed additives in meats by HPLC].
A simple and rapid multi-residue method was developed for the determination of 28 kinds of veterinary drugs and feed additives (drugs) in muscle of cattle, pig and chicken. The drugs were extracted with acetonitrile-water (95:5) in a homogenizer and ultrasonic generator. The extracted solution was poured into an alumina column and the drugs were eluted with acetonitrile-water (90:10). The eluate was washed with n-hexane saturated with acetonitrile and then evaporated. The drugs were separated on a Inertsil ODS-3V column (4.6 mm i.d. x 250 mm) with a gradient system of 0.1% phosphoric acid-acetonitrile as the mobile phase, with monitoring at 280 and 340 nm. The recoveries of the 26 kinds of drugs were over 60% from the meats fortified at 0.1 microg/g, and the quantification limits of most drugs were 0.01 microg/g. This proposed method was found to be effective and suitable for the screening of the above drugs in meats.
Chonan
Takao
T
Hokkaido Institute of Public Health, Kita-19, Nishi-12, Kita-ku, Sapporo 060-0819, Japan.
Fujimoto
Toru
T
Ueno
Ken-Ichi
K
Tazawa
Teijiro
T
Ogawa
Hiroshi
H
D000818:Animals; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005503:Food Additives / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D013552:Swine; D019155:Veterinary Drugs / Q000032:analysis*
D005503:Food Additives; D019155:Veterinary Drugs