title: Synthesis of peptide ribonucleic acid (PRNA)-DNA chimera and interaction with DNA and RNA.
abstract: Recently, we have demonstrated that effective control of the recognition behavior of peptide ribonucleic acid (PRNA) with complementary DNA is possible through the anti-to-synorientational change of pyrimidine nucleobase induced by borate ester formation. In this study, DNA-PRNA chimera was prepared by the solidphase synthesis. In the DNA-PRNA chimeras, both PRNA and DNA domains work as recognition sites for the complementary DNA/RNAs to form stable complex, while DNA-RNA hybrids formed in the DNA domains of DNA-PRNA chimera should be substrates to the hydrolysis by RNase H and PRNA moieties work as recognition control/switching devices and as inhibitor for the hydrolysis by exonucleases. Interaction of the DNA-PRNA chimera with DNA and RNA has been discussed.
authors.affiliation: Institute of Multidisciplinary Research for Advanced Materials,Tohoku University, 2-1-1, Katahira, Aobaku, Sendai 980-8577, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Wada
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Maeda
authors.forename: Nobuya
authors.initials: N
authors.lastname: Sawa
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sato
authors.forename: Hyongi
authors.initials: H
authors.lastname: Chon
authors.forename: Shigenori
authors.initials: S
authors.lastname: Kanaya
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Inoue
mesh_terms: D004247:DNA / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*
chemical_list: D020135:Peptide Nucleic Acids; D012313:RNA; D004247:DNA