title: Proteome and transcriptome analysis of cell death induced by 5-fluoro-2'-deoxyuridine.
abstract: 5-fluoro-2'-deoxyuridine (FUdR) inhibits thymidylate synthase. We have been investigated the molecular mechanisms of cell death in mouse mammary tumor FM3A cells, F28-7 strain and its mutant F28-7-A strain, after treated with FUdR. Previously, we have been reported that F28-7 strain induced DNA cleavage into chromosomal sized fragments and subsequently develop necrosis, but F28-7-A strain induced DNA cleavage into oligonucleosomal sized fragments and subsequently develop apoptosis after treated with FUdR. To understand the molecular mechanisms of regulate of two differential cell death necrosis and apoptosis, we identify cell death regulator by using proteome and transcriptome analysis. When compared with the proteome of F28-7 and F28-7-A strain after treated with FUdR, it was found that 5 proteins were up-regulated and 11 proteins were down-regulated in F28-7-A strain. Furthermore, transcriptome analysis shows that 94 genes were up-regulated and 164 genes were downregulated in F28-7-A strain. Identified proteins and genes were involved in various cellular processes such as cell cycle regulation, apoptosis, proliferation, and differentiation. Our results suggested that numerous features indicated the coordinated regulation of molecular networks from various aspects of necrosis or apoptosis at the proteome and transcriptome levels.
authors.affiliation: Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Sato
authors.forename: Eriko
authors.initials: E
authors.lastname: Miyazaki
authors.forename: Akito
authors.initials: A
authors.lastname: Satake
authors.forename: Akiko
authors.initials: A
authors.lastname: Hiramoto
authors.forename: Osamu
authors.initials: O
authors.lastname: Hiraoka
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Miyake
authors.forename: Hye-Sook
authors.initials: HS
authors.lastname: Kim
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Wataya
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D005467:Floxuridine / Q000494:pharmacology*; D020869:Gene Expression Profiling; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D051379:Mice; D009336:Necrosis; D040901:Proteomics
chemical_list: D000964:Antimetabolites, Antineoplastic; D005467:Floxuridine