Determination of procalcitonin concentration using the SphereLight 180 clinical auto-analyzer.
BACKGROUND
Procalcitonin (PCT) is a biomarker for the diagnosis of sepsis and bacterial infection diseases.


METHODS
A new fully automated SphereLight PCT (SL-PCT) assay system for PCT concentration in human serum or plasma by using SphereLight 180 (SL180, Olympus Corp.) analyzer was developed. The SL-PCT assay is based on chemiluminescent enzyme immunoassay.


RESULTS
A linear dose response relationship was observed up to 200 ng/ml PCT concentration. The detection limit of PCT concentration was 0.06 ng/ml. Endogenous substances, anticoagulants, sodium fluoride and drugs did not interfere with assay results. There was a good correlation between the present method and the manual method in serum and plasma samples.


CONCLUSIONS
These results indicate that the SL-PCT assay showed good performance in terms of the linearity, detection limit and precision. Use of this PCT measurement may improve the detection of sepsis and infectious disease.
Yamada
Hiroyuki
H
Osaka Research Laboratories, Wako Pure Chemical Industries Ltd, Amagasaki, Hyogo, Japan. h-yamada@wakousa.com
Matsuda
Shinjiro
S
Ushio
Yoshihiro
Y
Nakamura
Kenji
K
Kobatake
Shinzo
S
Satomura
Shinji
S
Matsuura
Shuji
S
D002116:Calcitonin / Q000097:blood*; D015740:Calcitonin Gene-Related Peptide; D006801:Humans; D008027:Light; D013677:Medical Laboratory Science / Q000379:methods*; D011498:Protein Precursors / Q000097:blood*; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000097:blood* / Q000175:diagnosis*
C000606589:CALCA protein, human; D011498:Protein Precursors; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide