title: yadBC of Yersinia pestis, a new virulence determinant for bubonic plague.
abstract: In all Yersinia pestis strains examined, the adhesin/invasin yadA gene is a pseudogene, yet Y. pestis is invasive for epithelial cells. To identify potential surface proteins that are structurally and functionally similar to YadA, we searched the Y. pestis genome for open reading frames with homology to yadA and found three: the bicistronic operon yadBC (YPO1387 and YPO1388 of Y. pestis CO92; y2786 and y2785 of Y. pestis KIM5), which encodes two putative surface proteins, and YPO0902, which lacks a signal sequence and likely is nonfunctional. In this study we characterized yadBC regulation and tested the importance of this operon for Y. pestis adherence, invasion, and virulence. We found that loss of yadBC caused a modest loss of invasiveness for epithelioid cells and a large decrease in virulence for bubonic plague but not for pneumonic plague in mice.
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536-0298, USA.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Forman
authors.forename: Christine R
authors.initials: CR
authors.lastname: Wulff
authors.forename: Tanya
authors.initials: T
authors.lastname: Myers-Morales
authors.forename: Clarissa
authors.initials: C
authors.lastname: Cowan
authors.forename: Robert D
authors.initials: RD
authors.lastname: Perry
authors.forename: Susan C
authors.initials: SC
authors.lastname: Straley
mesh_terms: D018829:Adhesins, Bacterial / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics; D002460:Cell Line; D005260:Female; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007928:Lethal Dose 50; D051379:Mice; D008810:Mice, Inbred C57BL; D010930:Plague / Q000382:microbiology*; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology*; D015010:Yersinia pestis / Q000235:genetics / Q000472:pathogenicity*
chemical_list: D018829:Adhesins, Bacterial; D037521:Virulence Factors; C526541:YadB protein, Yersinia pestis; C526542:YadC protein, Yersinia pestis