title: Pi-Pi complexation of bupivacaine and analogues with aromatic receptors: implications for overdose remediation.
abstract: The interaction of the important but often overdosed local anesthetic bupivacaine, its structural analogs 2,6-dimethylaniline, and N-methyl-2,6-dimethylacetanilide, and cocaine, with several electron deficient aromatic moieties were studied primarily by proton NMR and UV-visible spectroscopy. In solution, the anesthetic, its analogs and cocaine are electron donors and form pi-pi charge transfer complexes with strong aromatic acceptors, as monitored by the upfield changes induced in the NMR chemical shifts (delta) and red-shifted UV-vis wavelength (lamda max) absorbance of the acceptors. The equilibrium binding constant, K, was determined from the 1H NMR charge transfer induced chemical shift changes and used to calculate the free energy (deltaG) for complex formation of three acceptor-donor pairs. HPLC results indicate that the concentrations of free bupivacaine, its analogs and of cocaine are reduced from solution via binding to aromatic-functionalized silica.
authors.affiliation: Department of Chemistry, Clarkson University, Potsdam, NY, USA.
authors.forename: Evon
authors.initials: E
authors.lastname: Powell
authors.forename: Y H
authors.initials: YH
authors.lastname: Lee
authors.forename: Richard
authors.initials: R
authors.lastname: Partch
authors.forename: Donn
authors.initials: D
authors.lastname: Dennis
authors.forename: Timothy
authors.initials: T
authors.lastname: Morey
authors.forename: Manoj
authors.initials: M
authors.lastname: Varshney
mesh_terms: D000818:Animals; D002045:Bupivacaine / Q000009:adverse effects* / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry / Q000627:therapeutic use; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D003042:Cocaine / Q000009:adverse effects* / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D062787:Drug Overdose; D006801:Humans; D006841:Hydrocarbons, Aromatic / Q000737:chemistry; D016060:Sorption Detoxification / Q000379:methods*
chemical_list: D002614:Chelating Agents; D004337:Drug Carriers; D006841:Hydrocarbons, Aromatic; D003042:Cocaine; D002045:Bupivacaine