title: Detection of base pairing of an oxidatively damaged guanine using colloidal stability change of DNA-linked polymer micelles.
abstract: A DNA-linked polymer micelle was prepared through a phase transition of an amphiphilic copolymer consisting of single-stranded DNA as the hydrophilic part and thermo-responsive poly(N-isopropylacryl-amide) as the hydrophobic part. Duplex formation on the surface of micelle decreased the colloidal stability, whereas a terminal single-base mismatch at the interface between the DNA shell and the disperse medium increased the colloidal stability. Based on this change, we successfully detected base pairing of 7,8-dihydro-8-oxo-2'-deoxyguanine with adenine. This method will be useful for discovering a Watson-Crick or non Watson-Crick base pair of an unknown oxidative-damaged base with each four canonical one.
authors.affiliation: Bioengineering Laboratory, Discovery Research Institute, RIKEN, Saitama 351-0198, Japan. ttkrd@riken.jp
authors.forename: Tohru
authors.initials: T
authors.lastname: Takarada
authors.forename: Emi
authors.initials: E
authors.lastname: Imaizumi
authors.forename: Katsuhito
authors.initials: K
authors.lastname: Kino
authors.forename: Keitaro
authors.initials: K
authors.lastname: Yoshimoto
authors.forename: Shinzi
authors.initials: S
authors.lastname: Ogasawara
authors.forename: Naoki
authors.initials: N
authors.lastname: Kanayama
authors.forename: Mizuo
authors.initials: M
authors.lastname: Maeda
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D000225:Adenine / Q000737:chemistry; D020029:Base Pairing; D003102:Colloids; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000737:chemistry; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry; D008823:Micelles; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D003102:Colloids; D004277:DNA, Single-Stranded; D008823:Micelles; D011108:Polymers; C052970:poly-N-isopropylacrylamide; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine; D000225:Adenine