One-step immobilization of alkanethiol/glycolipid vesicles onto gold electrode: amperometric detection of Concanavalin A.
Functionalized vesicles composed of glycolipid and alkanethiol lipids have been immobilized onto gold surface through one-step self-assembly to construct an electrochemical biosensor for Concanavalin A (Con A) detection. Incorporation of alkanethiol lipid molecules into the vesicles allows for firm attachment of the vesicles onto a gold surface to form a sensing interface. At the same time, the introduction of alkanethiol lipid avoids cumbersome organic syntheses of sulfur-containing compound, making the biosensor greater applied prospect. Through the recognition of Con A by glycolipid which was immobilized on the surface of electrode, a decrease of electrochemical signal was observed. This decrease was restored when the electrode was immersed in a stronger binding solution such as glucose. The repeated usability of the novel sensor is excellent.
Guo
Caixin
C
Key Laboratory of Colloid and Interface Science, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Beijing, PR China.
Boullanger
Paul
P
Jiang
Long
L
Liu
Tao
T
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D003208:Concanavalin A / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D013438:Sulfhydryl Compounds; D003208:Concanavalin A; C013976:dodecylmercaptan; D006046:Gold; D000117:Acetylglucosamine