Predominant infection of CD150+ lymphocytes and dendritic cells during measles virus infection of macaques.
Measles virus (MV) is hypothesized to enter the host by infecting epithelial cells of the respiratory tract, followed by viremia mediated by infected monocytes. However, neither of these cell types express signaling lymphocyte activation molecule (CD150), which has been identified as the receptor for wild-type MV. We have infected rhesus and cynomolgus macaques with a recombinant MV strain expressing enhanced green fluorescent protein (EGFP); thus bringing together the optimal animal model for measles and a virus that can be detected with unprecedented sensitivity. Blood samples and broncho-alveolar lavages were collected every 3 d, and necropsies were performed upon euthanasia 9 or 15 d after infection. EGFP production by MV-infected cells was visualized macroscopically, in both living and sacrificed animals, and microscopically by confocal microscopy and FACS analysis. At the peak of viremia, EGFP fluorescence was detected in skin, respiratory and digestive tract, but most intensely in all lymphoid tissues. B- and T-lymphocytes expressing CD150 were the major target cells for MV infection. Highest percentages (up to 30%) of infected lymphocytes were detected in lymphoid tissues, and the virus preferentially targeted cells with a memory phenotype. Unexpectedly, circulating monocytes did not sustain productive MV infection. In peripheral tissues, large numbers of MV-infected CD11c+ MHC class-II+ myeloid dendritic cells were detected in conjunction with infected T-lymphocytes, suggesting transmission of MV between these cell types. Fluorescent imaging of MV infection in non-human primates demonstrated a crucial role for lymphocytes and dendritic cells in the pathogenesis of measles and measles-associated immunosuppression.
de Swart
Rik L
RL
Department of Virology, Erasmus MC, University Medical Center, Rotterdam, The Netherlands. r.deswart@erasmusmc.nl
Ludlow
Martin
M
de Witte
Lot
L
Yanagi
Yusuke
Y
van Amerongen
Geert
G
McQuaid
Stephen
S
Yüksel
Selma
S
Geijtenbeek
Teunis B H
TB
Duprex
W Paul
WP
Osterhaus
Albert D M E
AD
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D003713:Dendritic Cells / Q000378:metabolism / Q000821:virology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D049452:Green Fluorescent Proteins / Q000235:genetics; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000378:metabolism / Q000821:virology*; D008221:Lymphoid Tissue / Q000821:virology; D008251:Macaca; D008457:Measles / Q000378:metabolism / Q000821:virology*; D008459:Measles virus / Q000472:pathogenicity*; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009055:Mouth / Q000821:virology; D011956:Receptors, Cell Surface / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D012867:Skin / Q000821:virology
D015703:Antigens, CD; D011956:Receptors, Cell Surface; C000604059:SLAMF1 protein, human; D049452:Green Fluorescent Proteins; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1