Electrical measurements of bilayer membranes formed by Langmuir-Blodgett deposition on single-crystal silicon.
Bilayer membranes on solid supports are used for fundamental studies of biophysical properties and for the development of biosensors and other devices. Here we report on electrically addressable bilayer membranes formed by Langmuir-Blodgett (LB)-based deposition on single-crystal silicon. The incorporation of a polymer cushion ensures high lipid mobility in both the lower and upper leaflet, allowing the potential for combined investigations of electrical, structural, and dynamic characteristics of membrane-associated proteins. Impedance spectroscopy is used to demonstrate that the lipid bilayers are robust and reproducible with an impedance of about 10(4) Omega cm2 and a capacitance of about 0.8 microF cm(-2). The ability to characterize ion channels is demonstrated using the model system gramicidin. These results demonstrate that artificial bilayers formed by LB deposition have many unique advantages for electrical measurements of membranes and their components.
Nikolov
Vesselin
V
Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA.
Lin
Janice
J
Merzlyakov
Mikhail
M
Hristova
Kalina
K
Searson
Peter C
PC
D004560:Electricity; D008051:Lipid Bilayers; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
D008051:Lipid Bilayers; D012825:Silicon