title: [Effects of cinnamyl aldehyde on cell cycle and relafeol proteins expression in NIH3T3 cells].
abstract: OBJECTIVE
  To observe the effects of Cinnamyl aldehyde (CA) on NIH3T3 cell cycle and explore the possible mechanism further.
  
  
  METHODS
  Flowcytometry was used for observing cell cycle distribution. Expressions of proliferation cell nuclear antigen (PCNA) and Cyclin D1 protein in NIH3T3 cells were assessed by immunocytochemistry.
  
  
  RESULTS
  After culture with CA for 24 hours, the percentage of populations of S phase was enhanced by 3% (P < 0.05) and cell proliferation index (PrI, S + G2/M) was increased by 3.5% (P < 0.01) , but G2/M phase had no obvious changes. The expressions of Cyclin D1 and PCNA proteins were improved markly by CA compared with controlgroup (P < 0.01).
  
  
  CONCLUSIONS
  CA could promote more cells in G0/G1 phase into S phase, which may be related to the regulation of the expressions of PCNA and Cyclin D1.
authors.affiliation: Beijing Institute of Chinese Medicine, Beijing 100010, China.
authors.forename: Jing-xia
authors.initials: JX
authors.lastname: Zhao
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Xun
authors.initials: X
authors.lastname: Sheng
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Dai-ying
authors.initials: DY
authors.lastname: Liang
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D027424:Cinnamomum / Q000737:chemistry; D019938:Cyclin D1 / Q000378:metabolism*; D005434:Flow Cytometry; D007150:Immunohistochemistry; D051379:Mice; D041681:NIH 3T3 Cells; D010946:Plants, Medicinal / Q000737:chemistry; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D016196:S Phase / Q000187:drug effects
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D019938:Cyclin D1; D000171:Acrolein; C012843:cinnamaldehyde