SNPs typing based on the formation of fluorescent signaling DNA aptamers which bind to bile acids.
A simple and rapid method for the detection of single nucleotide polymorphisms (SNPs) is essential for the development of personalized medicine because SNPs correlate with some diseases and side effects of some drugs. Here we report a new method for the fluorescent detection of single nucleotide mutations that is based on the formation of cholic-acid binding DNA aptamers which form fully matched three-way junctions.
Kato
Teru
T
School of Bionics, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan. kato@bs.teu.ac.jp
Kamoto
Saori
S
Hyuga
Masumi
M
Karube
Isao
I
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics; D019826:Cholic Acid / Q000737:chemistry*; D065731:Cytochrome P-450 CYP2C19; D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006899:Mixed Function Oxygenases / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
D052157:Aptamers, Nucleotide; D015342:DNA Probes; D005456:Fluorescent Dyes; D006899:Mixed Function Oxygenases; D001189:Aryl Hydrocarbon Hydroxylases; C045793:CYP2C19 protein, human; D065731:Cytochrome P-450 CYP2C19; D019826:Cholic Acid