Natural antibody--complement dependent neutralization of bovine herpesvirus 4 by human serum.
In contrast to most gammaherpesviruses, Bovine herpesvirus 4 (BoHV-4) has a broad range of host species both in vitro and in vivo. Several in vitro studies demonstrated that some human cell lines are sensitive or even permissive to BoHV-4. These observations led to the hypothesis that cross-species transmission of BoHV-4 could lead to human infections. In the present study, we investigate the sensitivity of BoHV-4 to neutralization by naïve human sera in order to determine if humans exhibit innate anti-viral activities against this virus. Our results demonstrate that human sera from naïve individuals, in contrast to the sera of naïve subjects from various animal species, neutralize BoHV-4 efficiently. A series of complementary experiments were performed to unravel the mechanism(s) of this neutralization. The data obtained in this study demonstrates that human serum neutralizes BoHV-4 in a complement dependent manner activated by natural antibodies raised against the Galalpha1-3Galbeta1-4GlcNAc-R epitope expressed by bovine cells.
Machiels
Bénédicte
B
Immunology-Vaccinology (B43b), Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Bvd de Colonster 20, B-4000 Liège, Belgium.
Gillet
Laurent
L
Nascimento Brito
Sieberth Do
SD
Drion
Pierre
P
Delforge
Cédric
C
Nizet
Yannick
Y
Gianello
Pierre
P
Bona
Christophe
C
Costes
Bérénice
B
Markine-Goriaynoff
Nicolas
N
Vanderplasschen
Alain
A
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D002417:Cattle; D002460:Cell Line; D002522:Chlorocebus aethiops; D003167:Complement Activation; D003171:Complement Pathway, Classical / Q000276:immunology*; D026021:Herpesvirus 4, Bovine / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D007113:Immunity, Innate; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D009500:Neutralization Tests; D014312:Trisaccharides / Q000276:immunology; D014709:Vero Cells
D000914:Antibodies, Viral; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014312:Trisaccharides; C076569:alpha-galactosyl epitope