Expression of recombinant proteins in Pichia pastoris.
Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression. These elements include vectors, host strains, heterologous gene integration into the genome, secretion factors, and the glycosylation profile. In particular, we discuss and evaluate the recent progress in optimizing the fermentation process to improve the yield and stability of expressed proteins. Optimization can be achieved by controlling the medium composition, pH, temperature, and dissolved oxygen, as well as by methanol induction and feed mode.
Li
Pingzuo
P
Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, Shanghai, 200233, People's Republic of China.
Anumanthan
Anukanth
A
Gao
Xiu-Gong
XG
Ilangovan
Kuppusamy
K
Suzara
Vincent V
VV
Düzgüneş
Nejat
N
Renugopalakrishnan
V
V
D005285:Fermentation; D005822:Genetic Vectors; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000378:metabolism; D008957:Models, Genetic; D010100:Oxygen / Q000378:metabolism; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D013696:Temperature
D011994:Recombinant Proteins; D010100:Oxygen; D000432:Methanol