[Synergistic effects of acitretin and narrow-band ultraviolet-B in inducing retinoic acid receptor gamma mRNA expression in normal human keratinocytes].
OBJECTIVE
To investigate the changes in cell proliferation and retinoic acid receptor gamma (RARgamma) mRNA expression in normal human keratinocytes after acitretin treatment and/or narrow-band ultraviolet-B irradiation.


METHODS
Normal human keratinocytes were exposed to irradiation with 100 mJ/cm square NB-UVB and/or subsequent 12-hour incubation with 1x10(-6) mol/L acitretin, and the expression of RARgamma mRNA in the cells was examined using RT-PCR and real-time quantitative RT-PCR.


RESULTS
A 0.9- and a 2.3-fold increase in RARgamma mRNA expression was induced in the cells by exposure to 100 mJ/cm square NB-UVB and 10(-6) mol/L acitretin, respectively, and the expression was synergistically enhanced by 2.8-fold after their combined treatment.


CONCLUSIONS
Upregulated expression of RARgamma mRNA can be associated with keratinocyte growth inhibition after treatment with acitretin and NB-UVB irradiation.
Luo
Su-ju
SJ
Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. luosuju2005@163.com
Peng
Zhen-hui
ZH
Zheng
Yan
Y
Xu
Hao-xiang
HX
Zhou
Shao-na
SN
Li
Xiao-li
XL
Wang
Guo-rong
GR
D017255:Acitretin / Q000494:pharmacology*; D002478:Cells, Cultured; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000528:radiation effects*; D012333:RNA, Messenger / Q000378:metabolism; D018168:Receptors, Retinoic Acid / Q000378:metabolism*; D014466:Ultraviolet Rays; D000097768:Retinoic Acid Receptor gamma
D017255:Acitretin; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D000097768:Retinoic Acid Receptor gamma