title: The impact of liquid-based oral cytology on the diagnosis of oral squamous dysplasia and carcinoma.
abstract: OBJECTIVE
  Even though diagnostic oral exfoliative cytology is a useful, economical and practical tool in the diagnosis of oral dysplasia and carcinoma, it is not yet extensively used. The results of conventional exfoliative and liquid-based diagnostic cytology in oral potentially malignant lesions (PML) are herein reported and compared with the histological diagnosis.
  
  
  METHODS
  Either conventional (89) or liquid-based (384) exfoliative cytology was used for the diagnosis of oral dysplasia/carcinoma in 473 subjects and the results were compared with scalpel biopsy histology. Cells were collected using a Cytobrush device for conventional smears and with a dermatological curette for the liquid-based cytology. The 'curette technique' also allowed for the collection of 'accidental' tissue fragments, utilized as microbiopsies.
  
  
  RESULTS
  Histological diagnosis was squamous carcinoma in 96 of 473 cases, high-grade dysplasia (oral intraepithelial neoplasia two to three) in 24 and other lesions in 353 cases. The smears in the conventional cytology group were inadequate in 12.4%, with an 85.7% sensitivity and a 95.9% specificity. There were 8.8% of inadequate specimens in the liquid-based cytology group; sensitivity was 95.1% and specificity was 99.0%.
  
  
  CONCLUSIONS
  Although conventional cytology is useful when diagnosing oral PML (better sensitivity and predictive positive value if compared with the cervical smear test with similar specificity) and can improve the accuracy of histological diagnosis, liquid-based cytology gives better results, as it not only enhances both sensitivity and specificity, but also provides material for further investigation (AgNORs, DNA, microbiopsies, etc.).
authors.affiliation: Pathology Section, Department of Biomedical Sciences and Human Oncology of University of Turin, Turin, Italy. roberto.navone@unito.it
authors.forename: R
authors.initials: R
authors.lastname: Navone
authors.forename: P
authors.initials: P
authors.lastname: Burlo
authors.forename: A
authors.initials: A
authors.lastname: Pich
authors.forename: M
authors.initials: M
authors.lastname: Pentenero
authors.forename: R
authors.initials: R
authors.lastname: Broccoletti
authors.forename: A
authors.initials: A
authors.lastname: Marsico
authors.forename: S
authors.initials: S
authors.lastname: Gandolfo
mesh_terms: D002277:Carcinoma; D002278:Carcinoma in Situ / Q000175:diagnosis; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis*; D003581:Cytodiagnosis / Q000379:methods*; D003584:Cytological Techniques; D006801:Humans; D009059:Mouth Diseases / Q000175:diagnosis*; D009062:Mouth Neoplasms / Q000175:diagnosis*; D012680:Sensitivity and Specificity