[Study on technique of inducing microrhizome in Zingiber officinale].
OBJECTIVE
To study the effect of different factors on induction of microtubers in Zingiber officinale. These factors included NAA, PP333, 6-BA and sucrose.


METHODS
Orthogonal design and plant tissue culture technique were used.


CONCLUSIONS
Sucrose was the most important factor on the induction of microtubers, followed by PP333 and NAA. 6-BA was the factor which restrained the formation of microtubers. The optimal media to induce microtubers was MS + NAA 1.0 mg x L(-1) + PP333 0.2 mg x L(-1) + sucrose 8%.
Xue
Jian-ping
JP
College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China. xuejp2000@yahoo.com.cn
Huang
Yue-qin
YQ
Zhang
Ai-min
AM
D003470:Culture Media / Q000494:pharmacology; D020939:Zingiber officinale / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology; D010946:Plants, Medicinal / Q000254:growth & development*; D027343:Rhizome / Q000187:drug effects / Q000254:growth & development; D013395:Sucrose / Q000494:pharmacology; D046509:Tissue Culture Techniques / Q000379:methods
D003470:Culture Media; D010937:Plant Growth Regulators; D013395:Sucrose