[Observation of pollen tube germination of Platycodon grandiflorum by fluorescence microscopy].
OBJECTIVE
To determine the self-compatibility of Platycodon grandiflorum and the location of microspore germination on stigma.


METHODS
The microspore germination of self-pollination, self-plant-pollination and cross-pollination and the pollination microspore germination in and outside of stigma were observed with fluorescence microscopy.


RESULTS
Most pollens of self-pollination, self-plant-pollination and cross-pollination can germinate on the stigma, and after 24 hours, pollen tube entered into the ovary successfully. Pollinated on the outer-surface of stigma, microspores could not germinate, but on the inner-surface of the stigma when it dehisced most microspores can germinate.


CONCLUSIONS
The compatibility of self-plant-pollination of Platycodon grandiflorum is high. The microspore germination loci is on the inner-surface of the dehisced stigma.
Wei
Jian-he
JH
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Chinese Peking Union Medical College, Beijing 100094, China.
Ma
Feng-jie
FJ
Huang
Lu-qi
LQ
Yang
Cheng-min
CM
Sun
Zhi-tu
ZT
Dong
Qi-ting
QT
Cai
Ai-min
AM
D035264:Flowers / Q000254:growth & development; D008856:Microscopy, Fluorescence / Q000379:methods; D010946:Plants, Medicinal / Q000254:growth & development*; D031238:Platycodon / Q000254:growth & development*; D053205:Pollen Tube / Q000254:growth & development*; D054817:Pollination / Q000502:physiology