Hyperserotonaemia and reduced brain serotonin levels in NaS1 sulphate transporter null mice.
Sulphate (SO4) is conjugated to numerous endogenous compounds, including serotonin (5-HT). The NaS1 sulphate transporter is primarily expressed in the kidney, where it maintains blood SO4 concentrations. Previously, we generated NaS1 null (Nas1) mice, which have hyposulphataemia and decreased anxiety and locomotor activity. In this study, we investigated 5-HT and 5-hydroxyindole acetic acid (5-HIAA) concentrations, and 5-HT receptor mRNA levels. Nas1 mice exhibited a doubling in blood 5-HT concentration, but a 12% reduction in brain levels of 5-HT and 5-hydroxyindole acetic acid. Brain 5-HT1A and 5-HT1B receptor mRNA levels were increased by 50%, compared with wild-type mice. Our data indicate that decreased circulating SO4 concentrations modulate 5-HT neurotransmitter and receptor levels, in a manner consistent with the behavioural phenotypes of Nas1 mice.
Lee
Soohyun
S
School of Biomedical Sciences, University of Queensland, St Lucia, Australia.
Kesby
James P
JP
Muslim
Mohammed D
MD
Steane
Sarah E
SE
Eyles
Darryl W
DW
Dawson
Paul A
PA
Markovich
Daniel
D
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000503:physiopathology; D001923:Brain Chemistry / Q000235:genetics; D020739:Brain Diseases, Metabolic, Inborn / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation / Q000235:genetics; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000378:metabolism; D011985:Receptors, Serotonin / Q000235:genetics; D012701:Serotonin / Q000097:blood*; D000077003:Sodium Sulfate Cotransporter; D013457:Sulfur Compounds / Q000378:metabolism*; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics
D027682:Cation Transport Proteins; D012333:RNA, Messenger; D011985:Receptors, Serotonin; D000077003:Sodium Sulfate Cotransporter; D013457:Sulfur Compounds; D027981:Symporters; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid