Design of dual-labeled oligonucleotide probes for SNPs genotyping.
Our effort in designing base-discriminating fluorescence nucleosides (BDF), leads us to develop dual-labeled oligonucleotide probe in which the BDF nucleoside, (Py)U/(2-Ant)U act as the donor separated by a defined base pair distance from the acceptor, fluorescein, attached to 5'-end of the probe. Thus, a longer wavelength emission from acceptor might allow the probe to be used for SNP typing in chip based detection technology or in cell.
Saito
Yoshio
Y
Department of Marerials Chemistry and Engineering, School of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan. saito@mech.ce.nihon-u.ac.jp
Bag
Subhendu Sekhar
SS
Kodate
Satoshi
S
Suzuka
Isamu
I
D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D005838:Genotype; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide
C520208:5-(3-(1-pyrenecarboxamido)propynyl)-2'-deoxyuridine; C528343:5-(3-(2-anthracenecarboxamido)propynyl)-2'-deoxyuridine; D005452:Fluoresceins; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; C045132:4-carboxyfluorescein; D003857:Deoxyuridine