Screen-printed microfluidic device for electrochemical immunoassay.
In this paper, a new microfluidic array device has been fabricated with screen printing technology. In contrast to traditional microfabrication processes, our method is simple, inexpensive and also suitable for mass production. The device is used for sandwich-type electrochemical immunoassay, in which probes are covalently attached to the electrode surface via electropolymerized polypyrrole propylic acid (PPA) film. This novel microfluidic system enables the whole array preparation and detection processes, including the probe immobilization, sample injection, enzyme incubation and electrochemical detection, to be conducted in the sealed microchannels. For a demonstration, mouse IgG is selected as the target analyte and its detection is realized by sandwich ELISA with goat anti-mouse IgG, rat anti-mouse IgG (conjugated to alkaline phosphatase) and p-aminophenyl phosphate (PAPP) as the primary antibody, second antibody, and enzyme substrate, respectively. A detection limit of 10 ng mL(-1) (67 pM) is achieved with a dynamic range of 100 ng mL(-1)-10 microg mL(-1). In addition, anti-goat IgG is also immobilized as an alternative probe to test mouse IgG in the solution, in order to demonstrate the multiplexing capability as well as the specificity of the device. As expected, the electrochemical responses are much lower than that using anti-mouse IgG as the probe, indicating good selectivity of the immunoassay device. These results indicate a great promise toward the development of miniaturized, low-cost protein biochips for clinical, forensics, environmental, and pharmaceutical applications.
Dong
Hua
H
Center for Advanced Bionanosystems, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457.
Li
Chang-Ming
CM
Zhang
Yi-Fan
YF
Cao
Xiao-Dong
XD
Gan
Ye
Y
D000818:Animals; D002138:Calibration; D002244:Carbon / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D011422:Propionates / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D012680:Sensitivity and Specificity
D007074:Immunoglobulin G; D011422:Propionates; D011758:Pyrroles; C518858:polypyrrolepropylic acid; D002244:Carbon