Preparation and in vitro evaluation of bFGF-loaded chitosan nanoparticles.
The objective of our study was to prepare and characterize basic fibroblast growth factor (bFGF)-loaded nanoparticles. Protein-loaded chitosan nanoparticles were obtained by ionotropic gelation process based on the interaction between chitosan and tripolyphosphate (TPP). The protein-loading capacity and encapsulation efficiency were 0.021% and 27.388%, respectively. The bFGF-loaded nanoparticles have a mean diameter of 424 nm, a narrow size distribution, spherical shape and positive surface charges. In vitro release showed that the extent of release was 68% at 24 hr. The protein integrity was investigated by SDS-PAGE analysis that confirmed protein integrity was not affected by the encapsulation procedure and release conditions.
Cetin
Meltem
M
Department of Pharmaceutical Technology, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey.
Aktas
Yesim
Y
Vural
Imran
I
Capan
Yilmaz
Y
Dogan
Lale A
LA
Duman
Memed
M
Dalkara
Turgay
T
D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D004355:Drug Stability; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000493:pharmacokinetics; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011122:Polyphosphates / Q000737:chemistry; D055672:Static Electricity; D013499:Surface Properties
D003692:Delayed-Action Preparations; D011122:Polyphosphates; D016222:Fibroblast Growth Factor 2; D048271:Chitosan; C005692:triphosphoric acid