Increased expression of glycoprotein 340 in the ethmoid sinus mucosa of patients with chronic sinusitis.
OBJECTIVE
To investigate the expression and distribution of glycoprotein 340 (gp340), a secretory glycoprotein, in normal human sinus mucosa and inflammatory sinus mucosa and evaluate the possible effects of gp340 on the development of chronic sinusitis. Glycoprotein 340 was identified as a key element in the innate host defense mechanism on many mucosal surfaces and is directly involved in defense functions aimed at clearing gram-positive and gram-negative bacteria.


METHODS
Prospective study.


METHODS
Tertiary academic institution.


METHODS
Normal sinus mucosa was obtained from the ethmoid sinus mucosa of 8 patients with blowout fractures undergoing endoscopic reduction. Inflammatory sinus mucosa was taken from 25 patients with chronic polypoid sinusitis during endoscopic sinus surgery.


METHODS
Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemical analysis, and Western blotting were performed.


METHODS
The expression level and distributional pattern of gp340 in normal and inflammatory sinus mucosa were analyzed.


RESULTS
Transcripts of the gp340 gene were detected in all human sinus tissues analyzed by RT-PCR. Immunohistochemical analysis revealed that gp340 is mainly localized in submucosal gland of both normal and inflammatory sinus mucosa. Semiquantitative RT-PCR and Western blot analysis showed the increased expression levels of gp340 in the inflammatory sinus mucosa compared with the normal sinus mucosa.


CONCLUSIONS
These results suggest that gp340 may play a constitutive role in nasal defense and may be up-regulated in response to inflammation, participating in antimicrobial defense in chronic sinusitis.
Kim
Tae Hoon
TH
Department of Otorhinolaryngology-Head & Neck Surgery, College of Medicine, Korea University, 126-1 5 Ka Anam-Dong, SungBuk-Ku, Seoul, South Korea.
Lee
Sang Hag
SH
Lee
Heung Man
HM
Jung
Hak Hyun
HH
Lee
Seung Hoon
SH
Cho
Woo Sung
WS
Cinn
Young Gi
YG
Choe
Hwan
H
Kim
Moo Pil
MP
Yoo
Ik One
IO
Hwang
Ho Yeon
HY
D000328:Adult; D016022:Case-Control Studies; D002908:Chronic Disease; D005005:Ethmoid Sinus / Q000378:metabolism*; D005260:Female; D006801:Humans; D018928:Immunity, Mucosal / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D012852:Sinusitis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology
D012333:RNA, Messenger; D011971:Receptors, Immunologic; C116303:glycoprotein 340