title: RPEL motifs link the serum response factor cofactor MAL but not myocardin to Rho signaling via actin binding.
abstract: Myocardin (MC) family proteins are transcriptional coactivators for serum response factor (SRF). Each family member possesses a conserved N-terminal region containing three RPEL motifs (the "RPEL domain"). MAL/MKL1/myocardin-related transcription factor A is cytoplasmic, accumulating in the nucleus upon activation of Rho GTPase signaling, which alters interactions between G-actin and the RPEL domain. We demonstrate that MC, which is nuclear, does not shuttle through the cytoplasm and that the contrasting nucleocytoplasmic shuttling properties of MAL and MC are defined by their RPEL domains. We show that the MAL RPEL domain binds actin more avidly than that of MC and that the RPEL motif itself is an actin-binding element. RPEL1 and RPEL2 of MC bind actin weakly compared with those of MAL, while RPEL3 is of comparable and low affinity in the two proteins. Actin binding by all three motifs is required for MAL regulation. The differing behaviors of MAL and MC are specified by the RPEL1-RPEL2 unit, while RPEL3 can be exchanged between them. We propose that differential actin occupancy of multiple RPEL motifs regulates nucleocytoplasmic transport and activity of MAL.
authors.affiliation: Transcription Laboratory, Cancer Research UK London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3PX, United Kingdom. richard.treisman@cancer.org.uk
authors.forename: Sebastian
authors.initials: S
authors.lastname: Guettler
authors.forename: Maria K
authors.initials: MK
authors.lastname: Vartiainen
authors.forename: Francesc
authors.initials: F
authors.lastname: Miralles
authors.forename: Banafshe
authors.initials: B
authors.lastname: Larijani
authors.forename: Richard
authors.initials: R
authors.lastname: Treisman
mesh_terms: D000199:Actins / Q000378:metabolism*; D021581:Active Transport, Cell Nucleus; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D017124:Conserved Sequence; D003593:Cytoplasm / Q000378:metabolism; D005786:Gene Expression Regulation; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009185:Myelin Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D062806:Myelin and Lymphocyte-Associated Proteolipid Proteins; D041681:NIH 3T3 Cells; D009687:Nuclear Proteins / Q000378:metabolism; D010802:Phylogeny; D011485:Protein Binding; D011510:Proteolipids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D026362:Serum Response Factor / Q000378:metabolism*; D015398:Signal Transduction; D015534:Trans-Activators / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000378:metabolism*; D000099251:Myocardin
chemical_list: D000199:Actins; D026901:Membrane Transport Proteins; D009185:Myelin Proteins; D062806:Myelin and Lymphocyte-Associated Proteolipid Proteins; D009687:Nuclear Proteins; D011510:Proteolipids; D026362:Serum Response Factor; D015534:Trans-Activators; D020741:rho GTP-Binding Proteins; D000099251:Myocardin; C569027:Mal protein, mouse