title: Adjuvant effects of bacillus Calmette-Guerin DNA or CpG-oligonucleotide in the immune response to Taenia solium cysticercosis vaccine in porcine.
abstract: The immune stimulation properties of CpG-oligonucleotides (CpG-ODN) containing a central unmethylated CpG motif could be useful for vaccination against parasite infection. However, the high cost of synthetic CpG-ODN has limited its use in veterinary vaccines. In this study, we investigated whether genomic DNA derived from Mycobacterium bovis bacillus Calmette-Guerin (BCG-DNA) could be used as an effective adjuvant to enhance the immunogenicity and the protective capacity of recombinant cC1 antigen (rcC1) against pig cysticercosis. Pigs were vaccinated with rcC1 plus CpG-containing DNA adjuvants (BCG-DNA or CpG-ODN) or rcC1 alone. Immunization with rcC1 alone induced a Th1-biased response, whereas coadministration of rcC1 with BCG-DNA or CpG-ODN increased levels of IgG2, IFN-gamma, percentage of CD8+ and specific proliferation of peripheral blood mononuclear cells. Four weeks after the last immunization, pigs were infected with Taenia solium eggs. A high level of protection (81%) was induced by rcC1 immunization that was not significantly increased by the CpG-containing DNA. These data indicate that coadministration of rcC1 plus BCG-DNA or CpG-ODN significantly enhanced Th1 response but did not improve the level of the protection induced.
authors.affiliation: Department of Medical Genetics, The Second Military Medical University, Shanghai, China.
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Guo
authors.forename: D
authors.initials: D
authors.lastname: Wu
authors.forename: K-Y
authors.initials: KY
authors.lastname: Wang
authors.forename: S-H
authors.initials: SH
authors.lastname: Sun
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology; D001500:BCG Vaccine / Q000008:administration & dosage / Q000276:immunology* / Q000494:pharmacology; D003551:Cysticercosis / Q000517:prevention & control / Q000662:veterinary*; D007114:Immunization / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood; D008213:Lymphocyte Activation; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage / Q000276:immunology* / Q000494:pharmacology; D013552:Swine / Q000469:parasitology; D013553:Swine Diseases / Q000517:prevention & control*; D041201:Taenia solium / Q000276:immunology*; D018417:Th1 Cells / Q000187:drug effects; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D001500:BCG Vaccine; C408982:CPG-oligonucleotide; D007074:Immunoglobulin G; D009838:Oligodeoxyribonucleotides; D019444:Vaccines, DNA