AFM study of a new carrier based on PLA and salen copolymers for gene therapy.
The aim of this study was to synthesize novel biodegradable charged polymers to be used in DNA complexation for genetic delivery in different diseases. A new copolymer of PLA and complexed Schiff bases was synthesized in a several steps. This copolymer will be used as a nanocarrier. Also, AFM comparative studies in tapping mode were performed; on cationic copolymer and on PLA-Schiff base copolymer, on non-oriented and oriented film and on the DNA-cationic complex. The results indicated a difference in the topology and on phase picture of AFM film with or without cationic charge.
Nadeau
Véronique
V
Laboratoire de Nanotechnologie Pharmaceutique, Faculté de Pharmacie, Université de Montréal, (Québec), Canada.
Hildgen
Patrice
P
D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry; D005029:Ethylenediamines / Q000737:chemistry*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D019344:Lactic Acid / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008954:Models, Biological; D011091:Polyesters; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D012545:Schiff Bases / Q000138:chemical synthesis
D004337:Drug Carriers; D005029:Ethylenediamines; D011091:Polyesters; D011108:Polymers; D012545:Schiff Bases; D019344:Lactic Acid; C033616:poly(lactide); C011452:disalicylaldehyde ethylenediamine