title: Identification of boron transporter genes likely to be responsible for tolerance to boron toxicity in wheat and barley.
abstract: Tolerance to boron (B) toxicity in cereals is known to be associated with reduced tissue accumulation of B. Genes from roots of B-tolerant cultivars of wheat and barley with high similarities to previously reported B efflux transporters from Arabidopsis and rice were cloned. Expression of these genes was strongly correlated with the ability of tolerant genotypes to lower the concentration of B in roots. The gene from barley located to chromosome 4. Backcross lines containing a B tolerance locus on chromosome 4 showed tolerance in proportion to the level of expression of the transporter gene, whereas those lacking the locus were sensitive to B and had very low levels of gene expression. The results are consistent with a widespread mechanism of tolerance to high B based on efflux of B from root cells.
authors.affiliation: School of Earth and Environmental Sciences, University of Adelaide, Adelaide 5005, Australia. robert.reid@adelaide.edu.au
authors.forename: Rob
authors.initials: R
authors.lastname: Reid
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D001483:Base Sequence; D001895:Boron / Q000378:metabolism* / Q000633:toxicity; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D032461:Chromosomes, Plant; D017931:DNA Primers; D001467:Hordeum / Q000187:drug effects* / Q000378:metabolism; D008969:Molecular Sequence Data; D014908:Triticum / Q000187:drug effects* / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D001895:Boron