Photochemical properties of novel fluorescent probes for detection of DNA.
A variety of fluorescence-labeled DNA oligomers have been developed for gene analysis. We have studied on the development of a new functional fluorescent DNA probe for decrease in false positive signals. Thiazole orange derivatives were designed for the fluorescent label of DNA oligomers. The DNA oligomer containing a thiazole orange dye exhibited fluorescence with a higher intensity on hybridization with a complementary RNA, compared with that from the oligomer alone.
Ikeda
Shuji
S
The Frontier Research System, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama 351-0198, Japan.
Okamoto
Akimitsu
A
D052160:Benzothiazoles / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D010777:Photochemistry; D011804:Quinolines / Q000138:chemical synthesis / Q000737:chemistry*; D017422:Sequence Analysis, DNA; D013050:Spectrometry, Fluorescence
D052160:Benzothiazoles; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011804:Quinolines; C051000:thiazole orange