Hypotensive effect of UDP on intraocular pressure in rabbits.
Nucleotides can modify intraocular pressure (IOP). We have tested the ability of uridine-5'-diphosphate, UDP, for modulating IOP in New Zealand white rabbits. Uridine 5' diphosphate, UDP, reduced IOP by 82.9+/-2.6% compared to control. Dose-response analysis demonstrated a concentration dependent pattern which presented a pD(2) value of 7.57+/-1.45, equivalent to an EC(50) of 26.91 nM. Of all the tested P2 receptor antagonists, suramin, pyridoxalphosphate-6-azophenyl-2, 4-disulfonic acid (PPADS) and Reactive Blue 2 (RB-2), only the last two were able to reverse the action triggered by UDP. Altogether, UDP acting probably on P2Y(6) receptors present on the ciliary processes, can reduce intraocular pressure, indicating that this substance may be used for the treatment of ocular hypertension and glaucoma.
Markovskaya
Anna
A
Department of Pharmacology, Kazan State Medical University, Kazan, Russia.
Crooke
Almudena
A
Guzmán-Aranguez
Ana I
AI
Peral
Assumpta
A
Ziganshin
Airat U
AU
Pintor
Jesús
J
D000818:Animals; D002924:Ciliary Body / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005901:Glaucoma / Q000188:drug therapy; D007429:Intraocular Pressure / Q000187:drug effects*; D008297:Male; D009798:Ocular Hypertension / Q000188:drug therapy; D011817:Rabbits; D018048:Receptors, Purinergic P2 / Q000187:drug effects* / Q000378:metabolism; D014530:Uridine Diphosphate / Q000008:administration & dosage / Q000494:pharmacology*; D014544:Uridine Triphosphate / Q000494:pharmacology*
D018048:Receptors, Purinergic P2; C099845:purinoceptor P2Y6; D014530:Uridine Diphosphate; D014544:Uridine Triphosphate