title: Improved biological characteristics of poly(L-lactic acid) electrospun membrane by incorporation of multiwalled carbon nanotubes/hydroxyapatite nanoparticles.
abstract: Significant effort has been devoted to fabricating various biomaterials to satisfy specific clinical requirements. In this study, we developed a new type of guided tissue regeneration (GTR) membrane by electrospinning a suspension consisting of poly( l-lactic acid), multiwalled carbon nanotubes, and hydroxyapatite (PLLA/MWNTs/HA). MWNTs/HA nanoparticles were uniformly dispersed in the membranes, and the degradation characteristics were far improved. Cytologic research revealed that the PLLA/MWNTs/HA membrane enhanced the adhesion and proliferation of periodontal ligament cells (PDLCs) by 30% and inhibited the adhesion and proliferation of gingival epithelial cells by 30% also, compared with the control group. After PDLCs were seeded into the PLLA/MWNTs/HA membrane, cell/membrane composites were implanted into the leg muscle pouches of immunodeficient mice. Histologic examinations showed that PDLCs attached on the membranes functioned well in vivo. This new type of membrane shows excellent dual biological functions and satisfied the requirement of the GTR technique successfully in spite of a monolayer structure. Compared with other GTR membranes on sale or in research, the membrane can simplify the manufacturing process, reduce the fabrication cost, and avoid possible mistakes in clinical application. Moreover, it does not need to be taken out after surgery. PLLA/MWNTs/HA membranes have shown great potential for GTR and tissue engineering.
authors.affiliation: The Key Laboratory of Beijing City on Preparation and Processing of Novel Polymer, Beijing University of Chemistry and Technology, Beijing 100029, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Mei
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Zhong
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Yang
authors.forename: Xiangying
authors.initials: X
authors.lastname: Ouyang
authors.forename: Shen
authors.initials: S
authors.lastname: Zhang
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Hu
authors.forename: Qi
authors.initials: Q
authors.lastname: Ma
authors.forename: Jigui
authors.initials: J
authors.lastname: Lu
authors.forename: Seungkon
authors.initials: S
authors.lastname: Ryu
authors.forename: Xuliang
authors.initials: X
authors.lastname: Deng
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D017886:Durapatite / Q000138:chemical synthesis* / Q000378:metabolism; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods; D006801:Humans; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000378:metabolism; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism; D011091:Polyesters; D011108:Polymers / Q000138:chemical synthesis* / Q000378:metabolism
chemical_list: D037742:Nanotubes, Carbon; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D017886:Durapatite