title: Electro-acupuncture of Tsusanli and Shangchuhsu regulates gastric activity possibly through mediation of the vagus-solotary complex.
abstract: OBJECTIVE
  Acupuncture has been reportedly used to treat gastrointestinal diseases, however, its precise mechanism remains unknown.
  
  
  METHODS
  In our study, the effects and mechanism of electro-acupuncture (EA) at Tsusanli (ST 36), Shangchuhsu (ST 37) on regulation of gastric activity were observed.
  
  
  RESULTS
  EA at Tsusanli showed that gastric electric change was the most obvious, with significantly higher frequency and wave amplitude compared with that of the Shangchuhsu group and other groups. EA at Shangchuhsu demonstrated that the change of gastric electric level was much higher than that of the non-acupoint group and control group. After bilateral vagotomy, Tsusanlis was electro-acupunctured, the changes of electro-gastric graph (EGG) weren't significant with the control group. The frequency of electro-physiological activity in nucleus of solitary tract (NTS) and dorsal motor nucleus of the vagus nerve (DMV) in the Tsusanli group was markedly increased compared with that in the other group. Fos and GFAP expression in NTS and DMV in the Tsusanli group was significantly higher than that in other groups and control group. The results have indicated that EA at Tsusanli and Shangchuhsu cannot only regulate gastric activity, but also activate neurons and astrocytes in NTS and DMV. The effects on regulation and activation of EA at Tsusanli were very obvious.
  
  
  CONCLUSIONS
  Our study suggests that this electroacupuncture regulation of gastric activity may partially depend upon integrated nerve pathway and related central neurons and astrocytes in the vagus-solitary complex.
authors.affiliation: Department of Gastroenterology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China. Jingjie@fmmu.edu.cn
authors.forename: Jing-Jie
authors.initials: JJ
authors.lastname: Wang
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Qin
authors.forename: Liang-Wei
authors.initials: LW
authors.lastname: Chen
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Qiu
authors.forename: Li
authors.initials: L
authors.lastname: Duan
authors.forename: Rong
authors.initials: R
authors.lastname: Cao
authors.forename: Zhi-Ren
authors.initials: ZR
authors.lastname: Rao
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Huang
mesh_terms: D015670:Acupuncture Therapy / Q000379:methods*; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D015671:Electroacupuncture / Q000379:methods*; D004594:Electrophysiology; D005753:Gastric Mucosa / Q000378:metabolism; D005767:Gastrointestinal Diseases / Q000628:therapy*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods; D009046:Motor Neurons / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome; D014630:Vagus Nerve / Q000473:pathology*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D016760:Proto-Oncogene Proteins c-fos