title: Solution structure of the region 51-160 of human KIN17 reveals an atypical winged helix domain.
abstract: Human KIN17 is a 45-kDa eukaryotic DNA- and RNA-binding protein that plays an important role in nuclear metabolism and in particular in the general response to genotoxics. Its amino acids sequence contains a zinc finger motif (residues 28-50) within a 30-kDa N-terminal region conserved from yeast to human, and a 15-kDa C-terminal tandem of SH3-like subdomains (residues 268-393) only found in higher eukaryotes. Here we report the solution structure of the region 51-160 of human KIN17. We show that this fragment folds into a three-alpha-helix bundle packed against a three-stranded beta-sheet. It belongs to the winged helix (WH) family. Structural comparison with analogous WH domains reveals that KIN17 WH module presents an additional and highly conserved 3(10)-helix. Moreover, KIN17 WH helix H3 is not positively charged as in classical DNA-binding WH domains. Thus, human KIN17 region 51-160 might rather be involved in protein-protein interaction through its conserved surface centered on the 3(10)-helix.
authors.affiliation: Equipe de Chimie Organique et Biologie Structurale, IFRMP 23, CNRS UMR 6014, Université de Rouen, 76821 Mont-Saint-Aignan, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Carlier
authors.forename: Joël
authors.initials: J
authors.lastname: Couprie
authors.forename: Albane
authors.initials: A
authors.lastname: le Maire
authors.forename: Laure
authors.initials: L
authors.lastname: Guilhaudis
authors.forename: Isabelle
authors.initials: I
authors.lastname: Milazzo-Segalas
authors.forename: Marie
authors.initials: M
authors.lastname: Courçon
authors.forename: Mireille
authors.initials: M
authors.lastname: Moutiez
authors.forename: Muriel
authors.initials: M
authors.lastname: Gondry
authors.forename: Daniel
authors.initials: D
authors.lastname: Davoust
authors.forename: Bernard
authors.initials: B
authors.lastname: Gilquin
authors.forename: Sophie
authors.initials: S
authors.lastname: Zinn-Justin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification; D005822:Genetic Vectors; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins; D016415:Sequence Alignment; D051540:Winged-Helix Transcription Factors / Q000737:chemistry; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; C489487:KIN protein, human; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D051540:Winged-Helix Transcription Factors