title: A new method of culturing Leydig cells and prospects of their practical use in andrology.
abstract: We present a new method of obtaining a xenoculture enriched with Leydig cells from neonatal porcine testes providing 97-99% viability of cells in the culture. Genital extra-testicular transplantation of the obtained xenoculture was applied, which minimized traumaticity of surgery and the number of postoperative complications and had undoubted advantages over the subcapsular cell culture depot.
authors.affiliation: Central Interclinical Department of Andrology, I. M. Sechenov Moscow Medical Academy.
authors.forename: A A
authors.initials: AA
authors.lastname: Artyukhin
authors.forename: E I
authors.initials: EI
authors.lastname: Zaraiskii
mesh_terms: D000328:Adult; D000818:Animals; D000831:Animals, Newborn; D018929:Cell Culture Techniques; D002470:Cell Survival; D017690:Cell Transplantation; D002478:Cells, Cultured; D006801:Humans; D007246:Infertility / Q000276:immunology / Q000628:therapy; D007985:Leydig Cells / Q000166:cytology*; D008297:Male; D010126:Ozone / Q000627:therapeutic use; D013085:Spermatic Cord / Q000166:cytology; D013552:Swine; D014183:Transplantation, Heterologous
chemical_list: D010126:Ozone