title: Influence of factor IX on overall plasma coagulability and fibrinolytic potential as measured by global assay: monitoring in haemophilia B.
abstract: We sought to determine the influence of factor IX (FIX) deficiency upon overall coagulative and fibrinolytic capacities in plasma using the clot formation and lysis (CloFAL) assay, and to investigate the role of this global assay as an adjunctive monitoring tool in haemophilia B. CloFAL assay parameters were measured in vitro in platelet-poor plasma in relation to FIX activity and antigen (FIX:Ag), and were determined ex vivo among FIX-deficient patients (n = 41) in comparison to healthy individuals (n = 48). Supplementation of FIX-deficient plasma with FIX in vitro demonstrated a non-linear concentration dependence of FIX upon overall plasma coagulability. Ex vivo, coagulability was significantly decreased in FIX-deficient vs. healthy subjects among adults [median coagulation index (CI): 4% vs. 104% respectively; P < 0.001] and children (median CI: 9% vs. 63%; P < 0.001). Fibrinolytic capacity was increased in adult FIX-deficient vs. healthy subjects (median fibrinolytic index: 216% vs. 125%, respectively, P < 0.001), and was supported by a trend in shortened euglobulin lysis time (ELT). Severe haemophilia B patients showed heterogeneity in aberrant CloFAL assay waveforms, influenced partly by FIX:Ag levels. Patients with relatively preserved FIX:Ag (i.e. dysfunctional FIX) exhibited a shorter time to maximal amplitude in clot formation than those with type I deficiency. During patient treatment monitoring, markedly hypocoagulable CloFAL assay waveforms normalized following 100% correction with infused FIX. The CloFAL global assay detects FIX deficiency, demonstrates differences in coagulability between dysfunctional FIX and type I deficiency, and appears useful as an adjunctive test to routine FIX measurement in monitoring haemophilia B treatment.
authors.affiliation: Center for Cancer and Blood Disorders, Department of Pediatrics, University of Colorado at Denver and Health Sciences Center/The Children's Hospital, Aurora, CO, USA. neil.goldenberg@uchsc.edu
authors.forename: N A
authors.initials: NA
authors.lastname: Goldenberg
authors.forename: C
authors.initials: C
authors.lastname: Bombardier
authors.forename: W E
authors.initials: WE
authors.lastname: Hathaway
authors.forename: K
authors.initials: K
authors.lastname: McFarland
authors.forename: L
authors.initials: L
authors.lastname: Jacobson
authors.forename: M J
authors.initials: MJ
authors.lastname: Manco-Johnson
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001777:Blood Coagulation; D001780:Blood Coagulation Tests; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005164:Factor IX / Q000502:physiology*; D005342:Fibrinolysis; D002836:Hemophilia B / Q000097:blood / Q000145:classification* / Q000175:diagnosis*; D006801:Humans; D007223:Infant; D008875:Middle Aged; D012720:Severity of Illness Index
chemical_list: D005164:Factor IX