title: [Protective effect of thrombin precondition on astrocytes insulted by oxygen-glucose deprivation].
abstract: OBJECTIVE
  To explore the effect of thrombin precondition (TPC) on the rat cerebral astrocytes(As) cultured in oxygen-glucose deprivation (OGD).
  
  
  METHODS
  Astrocytes were pretreated with thrombin (TB) at various concentrations (0.005 approximately 5.000 kU/L), and then insulted by OGD. The cell damage and viability were evaluated by the lactate dehydrogenase (LDH) effusion rate and the 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) conversion method. Detection of apoptotic cells was determined by the flow cytometry technique. The glutamate uptake of astrocytes was studied with [3H]-glutamate incorporation.
  
  
  RESULTS
  OGD increased the LDH, decreased the cell viability, increased the number of apoptotic astrocytes, and decreased the glutamate uptake (P<0.01). While preconditioned with thrombin at the same condition, the LDH decreased, the cell viability increased, the percentage of apoptotic cells decreased, and the glutamate uptake increased (P<0.05). The maximum protective effect of thrombin was observed at 0.1 kU/L.
  
  
  CONCLUSIONS
  Low concentration of thrombin precondition (TPC) can protect the astrocytes from oxygen-glucose deprived injury, and attenuate its apoptosis in a dose-dependent manner.
authors.affiliation: Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410078.
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Luo
authors.forename: Li-xiang
authors.initials: LX
authors.lastname: Wu
authors.forename: Fa-yi
authors.initials: FY
authors.lastname: Liu
authors.forename: Bo-sheng
authors.initials: BS
authors.lastname: Huang
authors.forename: Fang
authors.initials: F
authors.lastname: He
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism*; D015687:Cell Hypoxia / Q000187:drug effects; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013917:Thrombin / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D018696:Neuroprotective Agents; D018698:Glutamic Acid; D013917:Thrombin; D005947:Glucose; D010100:Oxygen