Novel applications of locked nucleic acids.
Locked Nucleic Acid (LNA) nucleoside triphosphates were prepared and their substrate properties for different polymerases during primer extension and PCR experiments investigated. Phusion High Fidelity DNA polymerase and 9( degrees )Nm(TM) DNA polymerase readily accept LNA nucleoside 5'-triphosphates as substrates in primer extension assays. However, in PCR assays, However, in PCR assays, DNA 9oN(m) polymerase proved to be the best for amplification employing the LNA-A nucleotide.
Veedu
Rakesh N
RN
Nucleic Acid Center, University of Southern Denmark, Campusvej 55, DK - 5230 Odense M, Denmark.
Vester
Birte
B
Wengel
Jesper
J
D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D013698:Templates, Genetic
D009841:Oligonucleotides; C477371:locked nucleic acid; D004247:DNA; D004259:DNA-Directed DNA Polymerase