title: Genomic response programs of Saccharomyces cerevisiae following protoplasting and regeneration.
abstract: Global transcription profiling during regeneration of Saccharomyces cerevisiae protoplasts was explored. DNA microarrays measured the expression of 6388 genes and wall removal resulted initially in over-expression of 861 genes that decayed later on, a behaviour expected from a transient stress response. Kinetics of expression divided the genes into 25 clusters. Transcription of the genes from clusters 14-25 was initially up-regulated, suggesting that the grouped genes permitted cell adaptation to the removal of the wall. Clustering of genes involved in "wall structure and biosynthesis" showed that most of them had initially low levels of expression that increased along the process. Analysis by use of the T-profiler showed that the group of "structural components of the wall" was up-regulated after two hours and remained as such during the process. These results evoke the likeness and difference with the process occurring in Candida albicans.
authors.affiliation: Departament de Microbiología i Ecología, Facultat de Farmacia, Universitat de València, Avgda. Vicent Andrés Estellés s/n, 46100 Burjassot, València, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Castillo
authors.forename: Ana Isabel
authors.initials: AI
authors.lastname: Martínez
authors.forename: Samuel
authors.initials: S
authors.lastname: Gelis
authors.forename: José
authors.initials: J
authors.lastname: Ruiz-Herrera
authors.forename: Eulogio
authors.initials: E
authors.lastname: Valentín
authors.forename: Rafael
authors.initials: R
authors.lastname: Sentandreu
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D015966:Gene Expression Regulation, Fungal; D023281:Genomics / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D011523:Protoplasts / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000502:physiology
chemical_list: D029701:Saccharomyces cerevisiae Proteins