Ischemic preconditioning protects the dog kidney from ischemia-reperfusion injury.
OBJECTIVE
To evaluate the effects of unilateral ischemic insult and ischemic preconditioning (IPC) on renal histology in a canine model.


METHODS
30 dogs were randomized into 4 groups. In group A (5 male controls) and group B (5 female controls), ischemia was induced by clamping both left renal arteries for 40 min. Dogs in group C (10 male cases) or group D (10 female cases) underwent 5 min of arterial clamping and 10 min of declamping prior to the final 40-min ischemia induction. Renal biopsy was prepared 48 h later and microscopically examined.


RESULTS
The control groups (A and B) developed 40% frank necrosis, 60% moderate injury, and there was no intact renal tissue in this group with no difference between sexes. The IPC groups (C and D) revealed 55% moderate injury and 45% normal pathology; however, there was no frank necrosis among them. Better IPC protection in the female group was not statistically significant.


CONCLUSIONS
An IPC schedule of 5-min ischemia and 10-min reperfusion improves ischemia-reperfusion injury from subsequent prolonged ischemia in a canine model.
Salehipour
Mehdi
M
Division of Urology, Department of Surgery, Shiraz University of Medical Sciences, Shiraz, Iran. E-Mail salehipour@sums.ac.ir
Khezri
Abdolaziz
A
Monabbati
Ahmad
A
Jalaeian
Hamed
H
Kroup
Mohsen
M
Azizi
Valiallah
V
Tanideh
Nader
N
D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry; D019194:Ischemic Preconditioning / Q000379:methods*; D007668:Kidney / Q000098:blood supply; D008297:Male; D011336:Probability; D011897:Random Allocation; D012016:Reference Values; D015427:Reperfusion Injury / Q000473:pathology* / Q000517:prevention & control*; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric