title: Germline hypermethylation of the APC promoter is not a frequent cause of familial adenomatous polyposis in APC/MUTYH mutation negative families.
abstract: Familial adenomatous polyposis (FAP) is an autosomal dominant syndrome predisposing to colorectal cancer and affects 1 in 5-10,000 births. Inheritance of a mutant allele of the adenomatous polyposis coli (APC) gene is the cause of approximately 80% of FAP and 20-30% of an attenuated form of FAP (AFAP), whereas mutations in MUTYH account for a small proportion of the remaining cases. However, the genetic cause of FAP/AFAP in a significant number of families is not known, and cancer risk for individual members of these families cannot be assessed. There is, therefore, an acute need to identify the underlying genetic cause responsible for FAP/AFAP in APC/MUTYH mutation negative families. Hypermethylation of CpG islands in the promoter of tumor suppressor genes can result in gene silencing, has been shown to be functionally equivalent to genetic mutations and can be inherited. Moreover, APC promoter hypermethylation is observed in approximately 20% of sporadic colorectal tumors and correlates with the loss of gene expression. In our study, we used bisulfite treatment and direct sequencing of 2 regulatory regions of APC containing a total of 25 CpG dinucleotides, to investigate the possible role of germline hypermethylation of the APC promoter in FAP and AFAP families that were negative for APC and MUTYH mutations. Analysis of 21 FAP and 39 AFAP families did not identify signs of abnormal promoter methylation, indicating that this form of epigenetic silencing is not a common cause of FAP/AFAP. These results substantially contribute to clarify the potential role of germline epimutations as a cause of inherited predisposition to cancer.
authors.affiliation: Molecular Oncology Group, Molecular Biology and Biochemistry Research Center (CIBBIM), Nanomedicine Research Program, Vall d'Hebron Hospital Research Institute, Barcelona, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Romero-Giménez
authors.forename: Higinio
authors.initials: H
authors.lastname: Dopeso
authors.forename: Ignacio
authors.initials: I
authors.lastname: Blanco
authors.forename: Angel
authors.initials: A
authors.lastname: Guerra-Moreno
authors.forename: Sara
authors.initials: S
authors.lastname: Gonzalez
authors.forename: Stefanie
authors.initials: S
authors.lastname: Vogt
authors.forename: Stefan
authors.initials: S
authors.lastname: Aretz
authors.forename: Simo
authors.initials: S
authors.lastname: Schwartz
authors.forename: Gabriel
authors.initials: G
authors.lastname: Capella
authors.forename: Diego
authors.initials: D
authors.lastname: Arango
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000235:genetics*; D001483:Base Sequence; D018899:CpG Islands; D045647:DNA Glycosylases / Q000235:genetics*; D019175:DNA Methylation; D017931:DNA Primers; D017491:Genes, APC; D018095:Germ-Line Mutation; D006801:Humans; D011401:Promoter Regions, Genetic
chemical_list: D017931:DNA Primers; D045647:DNA Glycosylases; C061738:mutY adenine glycosylase