title: [The changes in blood flow in sciatic nerve after electrical injury in rabbit].
abstract: OBJECTIVE
  To study the changes in sciatic nerve blood flow and the expression of collagen type I after electric injury of rabbit nerve with different voltages.
  
  
  METHODS
  Thirty-six healty rabbits were randomized into 3 groups before receiving injury with electricity in voltages, i.e. 50 v, 75 v, and 100 v groups. The changes in blood flow of sciatic nerve were observed with Laser Doppler Flowmeter immediately after injury and 1, 4, 8 weeks after injury. The changes in the expression of collagen type I was observed by immunohistochemical method, and the positive expression rate was calculated.
  
  
  RESULTS
  The sciatic nerve blood flow increased in all groups immediately after electric injury. In the 75 v and 100v groups, the nerve blood flow [(53 +/- 3 ), (48 +/- 5) PU] was obviously lower than that of normal value [(62 +/- 4) PU, P < 0.05]. There was little collagen type I deposition in 50 v group, while brown collagenous fibers in epineurium and perineurium were observed in 75 v and 100v groups 4 and 8 weeks after injury. The expression of collagen type I in all groups were obviously higher than that of normal value, and that in 75v and 100 v groups were higher than that in 50 v group at bachl time-point (P < 0.01).
  
  
  CONCLUSIONS
  The restoration of sciatic nerve blood flow is postponed following by the injury with increase of the electrical voltage. The collagen deposition after electrical injury may be one of the reasons for nerve blood flow decrease.
authors.affiliation: Department of Burns and Plastic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, PR China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Xue-Yong
authors.initials: XY
authors.lastname: Li
authors.forename: Yue-Jun
authors.initials: YJ
authors.lastname: Li
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Fu
authors.forename: Wang-Zhou
authors.initials: WZ
authors.lastname: Li
authors.forename: Jin-Qing
authors.initials: JQ
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Xiao-Xing
authors.initials: XX
authors.lastname: Lv
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000096:biosynthesis; D004556:Electric Injuries / Q000097:blood* / Q000503:physiopathology*; D009416:Nerve Regeneration; D011817:Rabbits; D011897:Random Allocation; D012584:Sciatic Nerve / Q000098:blood supply* / Q000293:injuries*
chemical_list: D024042:Collagen Type I