Purification and characterization of arylmalonate decarboxylase from Achromobacter sp. KU1311.
We have isolated, purified and characterized arylmalonate decarboxylase (AMDase; EC 4.1.1.76). This is an unique enzyme that gives optically pure arylpropionates from the corresponding arylmalonates. Recently, we have screened similar enzyme producers from soil samples and succeeded in isolating Achromobacter sp. KU1311. The gene encoding the enzyme was cloned and sequenced. The AMDase gene consists of 720 nucleotides, which specifies a 240 amino acid protein with a relative molecular mass of 24,735. This enzyme was purified and its characteristics were compared with those of the hitherto known enzyme from Alcaligenes bronchisepticus KU1201.
Miyamoto
Kenji
K
Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
Yatake
Yoshito
Y
Tamura
Keisuke
K
Terao
Yosuke
Y
Ohta
Hiromichi
H
D042402:Achromobacter / Q000145:classification* / Q000201:enzymology*; D002262:Carboxy-Lyases / Q000737:chemistry* / Q000302:isolation & purification*; D004789:Enzyme Activation; D004795:Enzyme Stability; D013045:Species Specificity
D002262:Carboxy-Lyases; C071852:malonate decarboxylase