Detection of base pairing of an oxidatively damaged guanine using colloidal stability change of DNA-linked polymer micelles.
A DNA-linked polymer micelle was prepared through a phase transition of an amphiphilic copolymer consisting of single-stranded DNA as the hydrophilic part and thermo-responsive poly(N-isopropylacryl-amide) as the hydrophobic part. Duplex formation on the surface of micelle decreased the colloidal stability, whereas a terminal single-base mismatch at the interface between the DNA shell and the disperse medium increased the colloidal stability. Based on this change, we successfully detected base pairing of 7,8-dihydro-8-oxo-2'-deoxyguanine with adenine. This method will be useful for discovering a Watson-Crick or non Watson-Crick base pair of an unknown oxidative-damaged base with each four canonical one.
Takarada
Tohru
T
Bioengineering Laboratory, Discovery Research Institute, RIKEN, Saitama 351-0198, Japan. ttkrd@riken.jp
Imaizumi
Emi
E
Kino
Katsuhito
K
Yoshimoto
Keitaro
K
Ogasawara
Shinzi
S
Kanayama
Naoki
N
Maeda
Mizuo
M
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D000225:Adenine / Q000737:chemistry; D020029:Base Pairing; D003102:Colloids; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000737:chemistry; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry; D008823:Micelles; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*
D000178:Acrylamides; D000180:Acrylic Resins; D003102:Colloids; D004277:DNA, Single-Stranded; D008823:Micelles; D011108:Polymers; C052970:poly-N-isopropylacrylamide; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine; D000225:Adenine