title: Analysis of pain management drugs, specifically fentanyl, in hair: application to forensic specimens.
abstract: This article discusses the immunoassay screening of pain management drugs, and the mass spectrometric confirmation of fentanyl in human hair. Hair specimens were screened for fentanyl, opiates (including oxycodone), tramadol, propoxyphene, carisoprodol, methadone, and benzodiazepines and any positive results were confirmed using gas chromatography or liquid chromatography with mass spectral detection. The specific focus of the work was the determination of fentanyl in hair, since autopsy specimens were also available for comparison with hair concentrations. Using two-dimensional gas chromatography with electron impact mass spectrometric detection, fentanyl was confirmed in four of nine hair specimens collected at autopsy. The accuracy of the assay at 10 pg/mg was 95.17% and the inter-day and intra-day precision was 5.04 and 13.24%, respectively (n=5). The assay was linear over the range 5-200 pg/mg with a correlation of r(2)>0.99. The equation of the calibration curve forced through the origin was y=0.0053x and the limit of quantitation of the assay was 5 pg/mg. The fentanyl concentrations detected were 12, 17, 490, and 1930 pg/mg and the results were compared with toxicology from routine post-mortem analysis. The screening of pain management drugs in hair is useful in cases where other matrices may not be available, and in routine testing of hair for abused drugs.
authors.affiliation: Immunalysis Corporation, 829 Towne Center Drive, Pomona, CA 91767, USA. cmoore@immunalysis.com
authors.forename: C
authors.initials: C
authors.lastname: Moore
authors.forename: L
authors.initials: L
authors.lastname: Marinetti
authors.forename: C
authors.initials: C
authors.lastname: Coulter
authors.forename: K
authors.initials: K
authors.lastname: Crompton
mesh_terms: D000701:Analgesics, Opioid / Q000032:analysis*; D001569:Benzodiazepines / Q000032:analysis; D002328:Carisoprodol / Q000032:analysis; D011431:Dextropropoxyphene / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005283:Fentanyl / Q000032:analysis*; D053593:Forensic Toxicology; D008401:Gas Chromatography-Mass Spectrometry; D006197:Hair / Q000737:chemistry*; D006801:Humans; D008691:Methadone / Q000032:analysis; D010098:Oxycodone / Q000032:analysis; D015813:Substance Abuse Detection; D014147:Tramadol / Q000032:analysis
chemical_list: D000701:Analgesics, Opioid; D001569:Benzodiazepines; D002328:Carisoprodol; D014147:Tramadol; D010098:Oxycodone; D011431:Dextropropoxyphene; D008691:Methadone; D005283:Fentanyl