Antinociceptive effect of CNS peroxynitrite scavenger in a mouse model of orofacial pain.
Peroxynitrite (ONOO(-)) and species derived from it can oxidize and nitrate lipids, proteins and DNA leading to changes in signaling molecules. The present study was carried out to elucidate possible effects of CNS peroxynitrite in a mouse model of orofacial pain. Mice that received facial carrageenan injection + intracerebroventricular (i.c.v.) injection of the peroxynitrite scavenger [5,10,15,20-Tetrakis (4-sulfonatophenyl) porphyrinato iron (III), chloride] (FeTPPS) showed significantly fewer face wash strokes upon probing the inflamed area of the face with a von Frey hair at 6 h after injection, compared to mice that received facial carrageenan alone, or facial carrageenan injection + i.c.v. injection of saline. Mice that received i.c.v. injection of FeTPPS without facial carrageenan injection showed no significant difference in response to von Frey hair stimulation, compared to mice that received i.c.v. injection of saline without facial carrageenan injection. These results indicate an anti-nociceptive effect of the peroxynitrite scavenger FeTPPS in carrageenan induced facial pain but no effect on normal tactile sensation and point to an important role of CNS peroxynitrite in nociception.
Yeo
Jin-Fei
JF
Department of Oral and Maxillofacial Surgery, National University of Singapore, Singapore, 119260, Singapore.
Ling
Su-Fung
SF
Tang
Ning
N
Ong
Wei-Yi
WY
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D002351:Carrageenan / Q000009:adverse effects / Q000037:antagonists & inhibitors; D004195:Disease Models, Animal; D005157:Facial Pain / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D016166:Free Radical Scavengers / Q000494:pharmacology* / Q000627:therapeutic use; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000503:physiopathology; D018836:Inflammation Mediators / Q000009:adverse effects / Q000037:antagonists & inhibitors; D008297:Male; D008465:Mechanoreceptors / Q000187:drug effects / Q000502:physiology; D008665:Metalloporphyrins / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000378:metabolism; D009619:Nociceptors / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D010147:Pain Measurement / Q000187:drug effects; D017288:Pain Threshold / Q000187:drug effects / Q000502:physiology; D030421:Peroxynitrous Acid / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010812:Physical Stimulation; D014110:Touch / Q000187:drug effects / Q000502:physiology; D016896:Treatment Outcome; D014276:Trigeminal Nerve / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology
C466420:5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrinato iron(III) chloride; D016166:Free Radical Scavengers; D018836:Inflammation Mediators; D008665:Metalloporphyrins; D030421:Peroxynitrous Acid; D009569:Nitric Oxide; D002351:Carrageenan