title: Short tandem repeat typing technologies used in human identity testing.
abstract: Short tandem repeat (STR) typing methods are widely used today for human identity testing applications including forensic DNA analysis. Following multiplex PCR amplification, DNA samples containing the length-variant STR alleles are typically separated by capillary electrophoresis and genotyped by comparison to an allelic ladder supplied with a commercial kit. This article offers a brief perspective on the technologies and issues involved in STR typing.
authors.affiliation: Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8311, USA. john.butler@nist.gov
authors.forename: John M
authors.initials: JM
authors.lastname: Butler
mesh_terms: D001709:Biotechnology / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D053803:Forensic Genetics / Q000379:methods*; D018895:Microsatellite Repeats / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*