Thermus thermophilus tmRNA and trans-translation.
In trans-translation, ribosome switches the template for protein synthesis from an mRNA to tmRNA. The molecular mechanism of trans-translation remains mysterious. In order to clarify how tmRNA move through the ribosome, we developed in vitro systems to monitor trans-translation as well as translation, which are composed of ribosome, elongation factors, tmRNA and SmpB all from Thermus thermophilus. In these systems, the early steps of trans-translation including trans-transfer and the resume codon decoding could be monitored. Using these systems, the function of ribosomal protein S1 which has been suggested to be involved in trans-translation was investigated.
Takada
Kazuma
K
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 0368561, Japan.
Takemoto
Chie
C
Kawazoe
Masahito
M
Shirouzu
Mikako
M
Yokoyama
Shigeyuki
S
Muto
Akira
A
Himeno
Hyouta
H
D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*; D016963:Thermus thermophilus / Q000235:genetics* / Q000378:metabolism
D012329:RNA, Bacterial; D012269:Ribosomal Proteins; C015494:ribosomal protein S1; C096222:tmRNA