[Obtaining of isolated enterocytes from the small intestine of cattle fetuses under the control of biochemical indices of cell fractions].
The modification of chemical method for obtaining of isolated bovine fetal small intestine enterocytes is experimentally substantiated in the paper. The necessity of time reduction of incubation of small intestine in solution of chelating agents for obtaining the high-cleared fraction of absorptive cells with the low degree of their destruction is proved.
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D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D002469:Cell Separation; D020895:Enterocytes / Q000166:cytology / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D007421:Intestine, Small / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D013997:Time Factors