Application of the particle gel agglutination assay in the typing of single human leucocyte antigens.
We describe a simple and rapid particle gel agglutination assay (PaGIA) for typing of the human leucocyte antigens (HLA) HLA-A2, HLA-B7 and HLA-B27. Superparamagnetic streptavidin particles were coated with biotinylated monoclonal antibodies (MoAbs) to HLA-A2, HLA-B7 and HLA-B27. Anticoagulated whole blood samples from healthy blood donors (n = 118) with known HLA patterns were incubated with MoAb-coated particles, transferred into a standard ID-gel card, and subsequently centrifuged. Samples were evaluated macroscopically, with antigen-positive samples resulting in a visible agglutination reaction. A clear distinction could be made between all positive and negative samples tested. Fifty-seven samples were found to be positive for HLA-A2 (48%), 26 samples for HLA-B7 (22%) and 5 samples for HLA-B27 (4%).
Meyer
O
O
Charité-Universitätsmedizin Berlin, Institut für Transfusionsmedizin, Berlin, Germany.
Agaylan
A
A
Schönemann
C
C
Kiesewetter
H
H
Salama
A
A
D000372:Agglutination Tests; D000911:Antibodies, Monoclonal / Q000276:immunology; D052203:Ferrosoferric Oxide / Q000737:chemistry; D005782:Gels / Q000737:chemistry; D015789:HLA-A2 Antigen / Q000145:classification*; D015796:HLA-B27 Antigen / Q000145:classification*; D015793:HLA-B7 Antigen / Q000145:classification*; D006801:Humans; D008863:Microspheres; D019809:Streptavidin / Q000737:chemistry
D000911:Antibodies, Monoclonal; D005782:Gels; D015789:HLA-A2 Antigen; D015796:HLA-B27 Antigen; D015793:HLA-B7 Antigen; D019809:Streptavidin; D052203:Ferrosoferric Oxide