Effect of o,p'-DDD and Li+ on apoptotic DNA fragmentation in conventionally normal and tumour tissues of human adrenal cortex.
The actions of 1-(o-chlorophenyl)-1-(p-chlorophenyl)-2,2-dichloroethane (o,p'-DDD), potassium and lithium ions upon apoptotic processes in conventionally normal and tumour tissues of human adrenal cortex were studied. There was no effect of K+ on the apoptosis in tumour tissue. o,p'-DDD--the specific drug for conservative therapy of adrenocortical cancer--enhanced the apoptotic DNA fragmentation in all tested tissues. The conclusion was made that apoptosis may be involved in curative effect of o,p'-DDD in adrenal cortex. Lithium ions, which are used in clinic as antidepressant, inhibited the apoptosis in conventionally normal tissue and in most tumours. On the other hand, lithium enhanced the DNA fragmentation in the postoperative tissue of patients with Cushing disease. The possible mechanisms mediating lithium effects on the adrenal cortex are discussed.
Pushkarev
V M
VM
V. P. Komisarenko Institute of Endocrinology and Metabolism, Academy of Medical Sciences of Ukraine, Kyiv. endo@i.kiev.ua
Tronko
N D
ND
Kostyuchenko
N N
NN
Mikosha
A S
AS
D000302:Adrenal Cortex / Q000187:drug effects* / Q000473:pathology; D000306:Adrenal Cortex Neoplasms / Q000473:pathology*; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053938:DNA Fragmentation / Q000187:drug effects*; D006801:Humans; D018021:Lithium Chloride / Q000494:pharmacology*; D008939:Mitotane / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D018931:Antineoplastic Agents, Hormonal; D011189:Potassium Chloride; D008939:Mitotane; D018021:Lithium Chloride