Prevalence of Y chromosome microdeletions in infertile men with severe oligozoospermia in Serbia.
OBJECTIVE
The aim of this study was to determine the prevalence and type of microdeletions of the Y chromosome of men with severe oligozoospermia-ICSI candidates in the Serbian population and to compare our findings with those from other parts of the world.


METHODS
In all patients spermiogram has been performed in order to determine the sperm concentration. Patients were subjected to detailed clinical, endocrinological and cytogenetic examinations. Microdeletion analysis was performed by polymerase chain reaction (PCR) on 203 patients with normal cytogenetic findings. The STS markers tested in each case were sY84, sY86 (AZFa); sY127, sY134 (AZFb); sY254, sY255 (AZFc).


RESULTS
at least one of the STS markers was deleted in 11 of the 203 cases (5.4%).


CONCLUSIONS
AZFc microdeletions were identified with a rather high prevalence in men with severe oligozoospermia ICSI candidates in Serbian population.
Ristanovic
M
M
Institute of Human Genetics, Medical School, University of Belgrade, Belgrade, Serbia. ristanmomo@beotel.yu
Bunjevacki
V
V
Tulic
C
C
Novakovic
I
I
Perovic
V
V
Lukovic
L J
LJ
Milasin
J
J
D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D006801:Humans; D007248:Infertility, Male / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D009845:Oligospermia / Q000453:epidemiology / Q000235:genetics*; D015022:Yugoslavia / Q000453:epidemiology