Construction of target molecule sensing system using aptameric enzyme subunit based on PQQGDH activity.
We previously reported a novel target sensing element named aptameric enzyme subunit (AES). AES is composed of an aptamer binding to the target molecule and an aptamer affecting the enzymatic activity. In our previous work, we have succeeded in adenosine or DNA sensing by measuring the thrombin activity change resulting from adenosine or DNA binding to the AES. In this work, we have screened the pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) aptamers which affected the PQQGDH activity and have constructed an AES using the PQQGDH aptamer. As a model system, we connected the PQQGDH aptamer and an adenosine aptamer to make AES, and detected adenosine by measuring the PQQGDH activity change resulting from adenosine binding to the AES.
Ikebukuro
Kazunori
K
Department of Biotechnology & Life Science, Tokyo University of Agriculture & Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, Japan. ikebu@cc.tuat.ac.jp
Morita
Yo
Y
Yoshida
Wataru
W
Sode
Koji
K
D000241:Adenosine / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D005948:Glucose Dehydrogenases / Q000378:metabolism*; D021122:Protein Subunits / Q000378:metabolism
D052157:Aptamers, Nucleotide; D021122:Protein Subunits; D005948:Glucose Dehydrogenases; C033955:glucose dehydrogenase (pyrroloquinoline-quinone); D000241:Adenosine