[Analysis of beta-lactams-resistance genes in Pseudomonas aeruginosa in burn ward].
OBJECTIVE
To investigate the resistance genes and antibiotic resistance patterns against beta-lactams in Pseudomonas aeruginosa prevalent in burn ward.


METHODS
K-B method was performed to test bacterial resistance patterns against 9 species of beta-lactams in Pseudomonas aeruginosa isolated from wounds and dressings of the patient in burn wards. Seven species of resistance genes against beta-lactams were detected with PCR. Tazobactam-inhibited piperacillin resistance test was performed to study whether the above strains produce extended spectrum beta-lactams.


RESULTS
All 12 strains of bacteria with resistance genes detected were resistant to penicillin and cephalosporins (100%), among them 11 were resistant to all antibiotics. Tazobactam-inhibited piperacillin resistance test demonstrated that all strains with resistance genes were ESBLs.


CONCLUSIONS
High incidence of beta-lactams resistance genes is found in Pseudomonas aeruginosa isolated from burn ward, and they have close relationship with the occurrence of multiple drug-resistance.
Song
Qi-Fa
QF
Department of Microbiology, Centre for Disease Control and Prevention, Ningbo 315010, PR China. songqf@nbcdc.org.cn
Zheng
Jian
J
Lin
Hui
H
Xu
Jing-Ye
JY
Jin
Chun-Guang
CG
Li
Guo-Jun
GJ
D002053:Burn Units; D002056:Burns / Q000382:microbiology*; D005798:Genes, Bacterial; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D018440:beta-Lactam Resistance / Q000235:genetics*