Genotyping of Rhesus SCNT pluripotent stem cell lines.
Somatic cell nuclear transfer (SCNT) into enucleated oocytes has emerged as a technique that can be used to derive mouse embryonic stem cell lines with defined genotypes. In this issue Byrne et al. report the derivation of two SCNT Rhesus macaca male stem cell lines designated CRES-1 and CRES-2. Molecular studies detailed in their paper provides supporting evidence that the chromosome complement of CRES-1 and CRES-2 was genetically identical to the male cell donor nucleus and that the mitochondrial DNA originated from different recipient oocytes. In this validation paper, we independently confirm that both stem cell lines were indeed derived by SCNT.
Cram
David S
DS
Monash Immunology and Stem Cell Laboratories, Monash University, Clayton 3800, Victoria, Australia. david.cram@med.monash.edu.au
Song
Bi
B
Trounson
Alan O
AO
D000818:Animals; D002460:Cell Line; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D006801:Humans; D008253:Macaca mulatta / Q000235:genetics*; D008297:Male; D053652:Nuclear Transfer Techniques; D039904:Pluripotent Stem Cells / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D015203:Reproducibility of Results
D005819:Genetic Markers