title: Top-down mass spectrometry on low-resolution instruments: characterization of phosphopantetheinylated carrier domains in polyketide and non-ribosomal biosynthetic pathways.
abstract: Mass spectrometry (MS) is an important tool for studying non-ribosomal peptide, polyketide, and fatty acid biosynthesis. Here we describe a new approach using multi-stage tandem MS on a common ion trap instrument to obtain high-resolution measurements of the masses of substrates and intermediates bound to phosphopantetheinylated (holo) carrier proteins. In particular, we report the chemical formulas of 12 diagnostic MS(3) fragments of the phosphopantetheine moiety ejected from holo carrier proteins during MS(2). We demonstrate our method by observing the formation of holo-AcpC, a putative acyl carrier protein from Streptococcus agalactiae.
authors.affiliation: Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA 92093-0636, USA.
authors.forename: Dario
authors.initials: D
authors.lastname: Meluzzi
authors.forename: Wei Hao
authors.initials: WH
authors.lastname: Zheng
authors.forename: Mary
authors.initials: M
authors.lastname: Hensler
authors.forename: Victor
authors.initials: V
authors.lastname: Nizet
authors.forename: Pieter C
authors.initials: PC
authors.lastname: Dorrestein
mesh_terms: D000213:Acyl Carrier Protein / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry; D018942:Macrolides / Q000737:chemistry*; D013058:Mass Spectrometry / Q000295:instrumentation; D008958:Models, Molecular; D015394:Molecular Structure; D010204:Pantetheine / Q000031:analogs & derivatives* / Q000737:chemistry; D010453:Peptide Synthases / Q000737:chemistry*; D015398:Signal Transduction; D013292:Streptococcus agalactiae / Q000737:chemistry / Q000201:enzymology; D017855:Transferases (Other Substituted Phosphate Groups) / Q000737:chemistry
chemical_list: D000213:Acyl Carrier Protein; D001426:Bacterial Proteins; D018942:Macrolides; C082662:phosphopantetheinyl transferase; D010204:Pantetheine; D017855:Transferases (Other Substituted Phosphate Groups); D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase; C003129:4'-phosphopantetheine