Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure.
The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80 degrees C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.
Baert
Leen
L
Ghent University, Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation, Coupure links 653, 9000 Ghent, Belgium.
Wobus
Christiane E
CE
Van Coillie
Els
E
Thackray
Larissa B
LB
Debevere
Johan
J
Uyttendaele
Mieke
M
D000818:Animals; D017250:Caliciviridae Infections / Q000821:virology; D005759:Gastroenteritis / Q000821:virology; D006358:Hot Temperature; D051379:Mice; D029322:Norovirus / Q000235:genetics* / Q000254:growth & development; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013696:Temperature; D014162:Transfection / Q000379:methods*; D010948:Viral Plaque Assay / Q000379:methods*
D012367:RNA, Viral