Monitoring of Eco RI-catalyzed cleavage reaction of fluorescent-labeled heterochiral DNA.
We have found the unusual reactivity of a heterochiral oligodeoxynucleotide toward restriction endonuclease Eco RI. To conduct the kinetic analysis of the reaction, fluorescent-labeled single-stranded oligodeoxynucleotide molecular beacons were designed and synthesized. The beacons showed a remarkable fluorescence response by addition of Eco RI. The results promise that the beacon could be an effective tool for the kinetic analysis of Eco RI-catalyzed cleavage reaction of the heterochiral oligodeoxynucleotide.
Urata
Hidehito
H
Osaka university of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. urata@gly.oups.ac.jp
Tamaki
Chihiro
C
Matsuno
Miki
M
Wada
Shun-Ichi
S
Akagi
Masao
M
D002384:Catalysis; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI / Q000032:analysis* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*
D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D004247:DNA; D015246:Deoxyribonuclease EcoRI