title: Possible requirement of intercellular adhesion molecule-1 for invasion of gingival epithelial cells by Treponema medium.
abstract: Oral treponemes are members of the spirochete family of bacteria associated with periodontal diseases. In the present study, we demonstrate that intercellular adhesion molecule-1 (ICAM-1) on human gingival epithelial cells (HGEC) contributed to the invasion of Treponema medium, a medium-sized oral Treponema, into those cells. The quantity of T. medium in HGEC was found to peak at 2 h after inoculation and then decreased gradually. Immunofluorescence microscopy findings showed that the bacteria were colocalized with ICAM-1 on HGEC. Furthermore, knockdown of ICAM-1 in HGEC resulted in inhibition of T. medium invasion by RNA interference, whereas that of Toll-like receptor 2 did not. These results suggest that ICAM-1 may be required for the invasion of T. medium into HGEC, and they indicate that the molecule plays a principal role in the primary stages of the development and progression of chronic periodontitis.
authors.affiliation: Department of Oral Microbiology, Asahi University School of Dentistry, 1851-1 Hozumi, Mizuho, Gifu 501-0296, Japan.
authors.forename: Riyoko
authors.initials: R
authors.lastname: Tamai
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Asai
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kawabata
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Akisaka
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Ogawa
mesh_terms: D002460:Cell Line; D003470:Culture Media; D004847:Epithelial Cells / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D005881:Gingiva / Q000382:microbiology / Q000473:pathology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D010518:Periodontitis / Q000382:microbiology; D034622:RNA Interference; D014208:Treponema / Q000472:pathogenicity* / Q000648:ultrastructure; D014211:Treponemal Infections / Q000503:physiopathology
chemical_list: D003470:Culture Media; D018799:Intercellular Adhesion Molecule-1