[Genetic polymorphism analysis of 8 short tandem repeat loci on chromosome 2 in Chinese Han population in Shaanxi Province].
OBJECTIVE
To analyze the genetic polymorphism of 8 short tandem repeat (STR) loci on human chromosome 2 in Chinese Han population in Shaanxi Province.


METHODS
Blood samples anticoagulated with EDTA were collected from 176 unrelated Chinese Han individuals in Shaanxi Province. The DNA was extracted for PCR amplification of the relevant fragments, and the amplified products were analyzed using the ABI 3730 Genetic Analyzer.


RESULTS
On human chromosome 2, the loci D2S112, D2S162, D2S2330, D2S2216, D2S347, D2S259, D2S319 and D2S168 had 7, 11, 9, 8, 9, 9, 8 and 13 alleles, respectively, with 15, 33, 23, 18, 13, 12, 25 and 33 genotypes for the corresponding alleles. The genotype distribution of all the 8 loci met Hardy-Weinberg equilibrium. The heterozygosities for the 8 STR loci were 0.6985, 0.8274, 0.8042, 0.6816, 0.6541, 0.5213, 0.8432 and 0.8091, with polymorphic information content of 0.6911, 0.8199, 0.7891, 0.6809, 0.6388, 0.5187, 0.8372 and 0.8049, respectively.


CONCLUSIONS
The 8 loci on chromosome 2 have high heterozygosity and polymorphic information content in Chinese Han population, suggesting their value as useful genetic markers.
Ren
Feng-ling
FL
Department of Public Health, Key Laboratory of Environment and Genes Related to Diseases, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China. renfl@mail.xjtu.edu.cn
Guo
Xiong
X
Shi
Xiao-wei
XW
Lü
Ai-li
AL
Wang
Shi-jie
SJ
Zhang
Yin-gang
YG
D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D005828:Genetics, Population; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic