[Study on a novel vascular stent material (titanium oxide, Ti-O) coated with albumin and heparin: is it hemocompatible with fibrinogen].
The functional hemocompatibility between fibrinogen (FIG) and a novel vascular stent material (Ti-O film fixed with albumin and heparin) was investigated as follows: (1) Preparing the new biologic material (Ti-O) film; (2) Coating albumin and heparin on the Ti-O film; (3) Testing platelets (PL) adsorption; (4) Determining FIG adhesion number by use of enzyme linked immunoassay (ELISA); (5) Implanting the films from the test group of Ti-O film and from the comparison group of stainless steel (SS) film into the left and right femoral arteries respectively in 4 dogs. It was proved that albumin and heparin were fixed on Ti-O film. After 6 months, the femoral arteries of the dogs were resected. In the test group of Ti-O film coated with albumin and heparin, few PL adhered to the coat, their form did not change, and no thrombus was found by scanning electron microscopy; the result was better than that of plain Ti-O film, and was much better than that of SS film. Ti-O maintained normal transformation condition of FIG, and no C terminal of gamma chain in FIG was revealed. As it is known whether the hemocompatibility of a biomaterial is good depends upon its adsorption of FIG, and Ti-O has excellent reaction on albumin and heparin by chemical processes. In this study, the Ti-O film coated with albumin and heparin further reduced the absorption of FIG and PL when compared against the plain Ti-O film. So the Ti-O film coated with albumin and heparin has the insistent and permanent anticoagulant character.
Song
Jie
J
Department of Cardiology, Nanjing Drum Tower Hospital Affiliated to School of Medicine Nanjing University, Nanjing 210008, China.
Wu
Xi
X
Huang
Nan
N
Xu
Biao
B
Jing
Fengjuan
F
Chen
Junying
J
Leng
Yongxiang
Y
Yang
Ping
P
Wang
Jin
J
Sun
Hong
H
Zhao
Ansha
A
D000418:Albumins / Q000737:chemistry*; D000818:Animals; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D004285:Dogs; D005340:Fibrinogen / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods; D011474:Prosthesis Design; D015607:Stents; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D000418:Albumins; D020099:Coated Materials, Biocompatible; C009495:titanium dioxide; D005340:Fibrinogen; D006493:Heparin; D014025:Titanium