title: Three-dimensional cell and tissue patterning in a strained fibrin gel system.
abstract: Techniques developed for the in vitro reproduction of three-dimensional (3D) biomimetic tissue will be valuable for investigating changes in cell function in tissues and for fabricating cell/matrix composites for applications in tissue engineering techniques. In this study, we show that the simple application of a continuous strain to a fibrin gel facilitates the development of fibril alignment and bundle-like structures in the fibrin gel in the direction of the applied strain. Myoblasts cultured in this gel also exhibited well-aligned cell patterning in a direction parallel to the direction of the strain. Interestingly, the direction of cell proliferation was identical to that of cell alignment. Finally, the oriented cells formed linear groups that were aligned parallel to the direction of the strain and replicated the native skeletal muscle cell patterning. In addition, vein endothelial cells formed a linear, aligned vessel-like structure in this system. Thus, the system enables the in vitro reproduction of 3D aligned cell sets replicating biological tissue patterns.
authors.affiliation: Department of Oromaxillofacial Regeneration, Osaka University, Suita, Japan. tmatsu@dent.osaka-u.ac.jp
authors.forename: Takuya
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Sasaki
authors.forename: Eben
authors.initials: E
authors.lastname: Alsberg
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Egusa
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yatani
authors.forename: Taiji
authors.initials: T
authors.lastname: Sohmura
mesh_terms: D005337:Fibrin; D005782:Gels; D006801:Humans; D019344:Lactic Acid; D018625:Microscopy, Atomic Force; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013718:Tensile Strength
chemical_list: D005782:Gels; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005337:Fibrin