[Influence of PGLA degradable products on main physiologic function].
The biological safety of degradable products is a key point in biological evaluation of biodegradable materials. This paper deals with an investigation of the influence of PGLA degradable products on physiologic function of liver and kidney by detecting the "before-after change" of some biochemical indexes with the use of self-control method. PGLA materials of different sizes were implanted into the subcutaneous tissue of Wistar rats and New Zealand rabbits respectively. Also for the purpose of group comparison, only surgical operation was performed on other animal at the same site (control group). The concentrations of urea nitrogen, uric creatinine, blood urea nitrogen (BUN), carbamide (Cr) and serum glutamio pyruvic transaminase (GPT) were measured before and after implantation (from 2 w to 10 w). The results showed the values of urea nitrogen and uric creatinine were significantly increased at 2-3 weeks after implantation (P < 0.01). After 2 weeks of implantation, the concentration of GPT was slightly decreased (P < 0.05). The BUN and Cr were remarkably increased (P < 0.01). But at the 4th week, all the values returned to normal and kept stable thenceforth. All the biochemical indexes in the control animal exhibited no abnormal change after operation. These results indicated: (1) There are no permanent harmful effects of PGLA degradable products on the function of liver and kidney; (2) By means of self-control and measurements of some biochemical indexes in the blood or urea, the method is suitable for evaluating the biological safety of degradable products; (3) The method has the advantages for evaluating the bioaccept ability of degradable products during the life period, it is objective and sensitive, the number of animal can be decreased, the process of dynamic change in vivo can be observed directly, and the effect of degradable products on physical function can be evaluated. Therefore, the method can provide a new approach for biological evaluation of biodegradable materials.
Sun
Jiao
J
No. 9 People's Hospital, Medical College of Shanghai Jiaotong University, Shanghai Biomaterial Research and Test Center, Shanghai 200023, China. jiaosun59@yahoo.com
Guo
Shangchun
S
D020341:Absorbable Implants / Q000009:adverse effects*; D000818:Animals; D001673:Biodegradation, Environmental; D005260:Female; D006016:Glycolates / Q000009:adverse effects* / Q000737:chemistry; D020007:Implants, Experimental; D007668:Kidney / Q000502:physiology*; D019344:Lactic Acid; D008099:Liver / Q000502:physiology*; D008297:Male; D008422:Materials Testing; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
D006016:Glycolates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid