Effect of a Bowman-Birk proteinase inhibitor from Phaseolus coccineus on Hypothenemus hampei gut proteinases in vitro.
The coffee berry borer, Hypothenemus hampei (Ferrari), is an important devastating coffee pest worldwide. Both trypsin and chymotrypsin enzyme activities from H. hampei larval midgut can be inactivated by proteinaceous enzyme-inhibitors. A serine proteinase inhibitor belonging to the Bowman-Birk class was purified from a wild accession of Phaseolus coccineus L. seeds. The inhibitor (PcBBI1) is a cysteine-rich protein that is heat-stable at alkaline pH. MALDI-TOF/MS analysis showed that PcBBI1 occurs in seeds as a monomer (8689 Da) or dimer (17,378 Da). Using in vitro inhibition assays, it was found that PcBBI1 has a high inhibitory activity against H. hampei trypsin-like enzymes, bovine pancreatic chymotrypsin, and trypsin. According to this, PcBBI1 could be a promising tool to make genetically modified coffee with resistance to coffee berry borer.
de Azevedo Pereira
Railene
R
Embrapa Recursos Genéticos e Biotecnologia, PqEB, W5 Norte Final, Brasília - DF, 70770-900, Brazil.
Valencia-Jiménez
Arnubio
A
Magalhães
Cláudio Picanço
CP
Prates
Maura Vianna
MV
Melo
Jorge Alex Taquita
JA
de Lima
Liziane Maria
LM
de Sales
Maurício Pereira
MP
Tempel Nakasu
Erich Yukio
EY
da Silva
Maria Cristina Mattar
MC
Grossi-de-Sá
Maria Fátima
MF
D000595:Amino Acid Sequence; D000818:Animals; D001517:Coleoptera / Q000201:enzymology*; D007814:Larva / Q000201:enzymology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism*; D027805:Phaseolus / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D015842:Serine Proteinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*
D015842:Serine Proteinase Inhibitors; D010447:Peptide Hydrolases