Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Plants use light to optimize growth and development. The photoreceptor phytochrome A (phyA) mediates various far-red light-induced responses. We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
Lin
Rongcheng
R
Boyce Thompson Institute for Plant Research (BTI), Cornell University, Ithaca, NY 14853, USA.
Ding
Lei
L
Casola
Claudio
C
Ripoll
Daniel R
DR
Feschotte
Cédric
C
Wang
Haiyang
H
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008027:Light; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D010834:Phytochrome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051942:Phytochrome A / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D019895:Transposases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; C120919:FAR1 protein, Arabidopsis; C524723:FHL protein, Arabidopsis; C441269:FHY1 protein, Arabidopsis; C452847:FHY3 protein, Arabidopsis; D009687:Nuclear Proteins; C497973:PHYA protein, Arabidopsis; D051942:Phytochrome A; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D010834:Phytochrome; D019895:Transposases