title: Kinetic analysis of the effects of translation enhancers in translation initiation.
abstract: Translation initiation is the most important step within a series of protein biosynthesis processes because the incorporation of ribosomes to a mRNA mainly determines efficiencies of translation. In bacteria, translation enhancers located on the 5' upstream of the Shine-Dargalno (SD) sequence on mRNAs are known to accelerate the efficiency of protein biosynthesis. To investigate the role of translation enhancers in translation initiation, we analyzed binding kinetics of a 30S ribosomal subunit to a mRNA immobilized on a 27 MHz quartz-crystal microbalance (QCM). The association constant (Ka) was rather low for the mRNA including a translation enhancer sequence compared with that for the mRNA without translation enhancers. These kinetic parameters suggest that translation enhancers destabilize the ribosome-mRNA complex on an SD sequence to move on the next step of decoding its mRNA.
authors.affiliation: Department of Biomolecular Engineering, Frontier Collaborative Research Center, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Shuntaro
authors.initials: S
authors.lastname: Takahashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Furusawa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Takuya
authors.initials: T
authors.lastname: Ueda
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Okahata
mesh_terms: D020121:5' Untranslated Regions / Q000737:chemistry*; D001483:Base Sequence; D007700:Kinetics; D008969:Molecular Sequence Data; D010442:Peptide Chain Initiation, Translational; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D038621:Regulatory Sequences, Ribonucleic Acid; D054680:Ribosome Subunits, Small, Bacterial / Q000378:metabolism*
chemical_list: D020121:5' Untranslated Regions; D012333:RNA, Messenger; D038621:Regulatory Sequences, Ribonucleic Acid