title: Diagnostic multiplex PCR assay for the identification of the Liverpool, Midlands 1 and Manchester CF epidemic strains of Pseudomonas aeruginosa.
abstract: Individual PCR amplification tests have been developed for three UK CF epidemic strains, the Liverpool epidemic strain (LES), Midlands 1 and the Manchester epidemic strain (MES). We report a simple diagnostic multiplex PCR test that can be used to screen for all three of these strains. To evaluate the test, we screened collections of LES, MES and Midlands 1 isolates, along with various CF and non-CF non-epidemic Pseudomonas aeruginosa strains. The test was 100% sensitive and 100% specific in the identification of these UK CF epidemic strains.
authors.affiliation: Division of Medical Microbiology, University of Liverpool, Daulby Street, Liverpool L69 3GA, United Kingdom.
authors.forename: Joanne L
authors.initials: JL
authors.lastname: Fothergill
authors.forename: Anna L
authors.initials: AL
authors.lastname: Upton
authors.forename: Tyrone L
authors.initials: TL
authors.lastname: Pitt
authors.forename: C Anthony
authors.initials: CA
authors.lastname: Hart
authors.forename: Craig
authors.initials: C
authors.lastname: Winstanley
mesh_terms: D015897:Comorbidity; D003550:Cystic Fibrosis / Q000453:epidemiology*; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011552:Pseudomonas Infections / Q000453:epidemiology*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000302:isolation & purification; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers