[Metabolic remodeling and the alteration of related receptors expressions in failing heart].
OBJECTIVE
To explore the metabolic remodeling and its correlative mechanisms in human failing heart by contrasting the content of substrate, the activity of correlative enzyme and the mRNA and protein expression of beta(3)-adrenoceptor and peroxisome proliferator-activate receptor alpha (PPARalpha).


METHODS
Cardo ac tossie samples were talem from 20 patients with heart failure of valvular heart disease and undergoing valvuloplasty operation as well as 6 subjects being killed in accidents (as control). The levels of free fat acid (FFA) and the activity of succinate dehydrogenase (SDH), Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase of the myocardial tissue were measured. Protein and mRNA expression of beta(3)-adrenergic receptor and peroxisome proliferator-activated receptor alpha (PPARalpha) in myocardial tissue were analyzed using reverse transcriptase-polymerase chain reaction and Western-blot respectively.


RESULTS
In the failing hearts, the contents of FA and expression of beta 3 adrenoceptor mRNA and protein were obviously higher than those in the control group, the activity of SDH, Na(+)-K(+)-ATPase, Ca(2+)-Mg(2+)-ATPase and expression of PPARalpha mRNA and protein were on the contrary.


CONCLUSIONS
The human failing myocardium is characterized by metabolic remodeling and it maybe concerned with the upregulation of beta3 adrenoceptor and downregulation of PPARalpha.
Tian
Ying
Y
Department of Cardiology, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China.
Zhu
Shan-Jun
SJ
Wang
Jiang
J
Li
Zhen-Kui
ZK
Song
Rong
R
Nie
Ling
L
D000328:Adult; D015153:Blotting, Western; D005230:Fatty Acids, Nonesterified / Q000096:biosynthesis; D005260:Female; D015870:Gene Expression; D006333:Heart Failure / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D019918:Heart Valve Prosthesis Implantation; D006801:Humans; D008297:Male; D047492:Peroxisome Proliferator-Activated Receptors / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D022702:Receptors, Adrenergic, beta-3 / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020257:Ventricular Remodeling
D005230:Fatty Acids, Nonesterified; D047492:Peroxisome Proliferator-Activated Receptors; D012333:RNA, Messenger; D022702:Receptors, Adrenergic, beta-3