High performance liquid chromatographic method for the determination and pharmacokinetic studies of oxyresveratrol and resveratrol in rat plasma after oral administration of Smilax china extract.
A sensitive and simple HPLC method has been developed and validated for the determination of oxyresveratrol (trans-2,4,3',5'-tetrahydroxystilbene, OXY) and resveratrol (trans-3,5,4'-trihydroxystilbene, RES) in rat plasma. The plasma samples were extracted with ethyl acetate and analyzed using HPLC on an Aglient Zorbax SB-C(18) column (250 x 4.6 mm, 5 microm) at a wavelength 320 nm, with a linear gradient of (A) acetonitrile and (B) 0.5% aqueous acetic acid (v/v), at a flow rate of 1.0 mL/min. The method was linear over the range of 0.1265-25.3 microg/mL for OXY and 0.117-23.4 microg/mL for RES. The extraction recovery for OXY, RES and internal standard ranged from 71.1 to 88.3%. The intra- and inter-day precisions were better than 10%, and the accuracy ranged from 89 to 108%. The validated method was used to study the pharmacokinetic profiles of OXY and RES in rat plasma after oral administration of Smilax china root extract.
Huang
Huilian
H
College of Traditional Chinese Material Medica, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
Zhang
Jinqiang
J
Chen
Guangtong
G
Lu
Zhiqiang
Z
Wang
Xiaoming
X
Sha
Na
N
Shao
Bo
B
Li
Ping
P
Guo
De-an
DA
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D015394:Molecular Structure; D010936:Plant Extracts / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D000077185:Resveratrol; D032281:Smilax / Q000737:chemistry*; D013267:Stilbenes / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics
D010936:Plant Extracts; D013267:Stilbenes; C034912:puag-haad; D000077185:Resveratrol