Sensing of nucleic acid sequences using unmodified nucleic acid as a probe.
We present a strategy to generate a light-up fluorophore-aptamer pair. The strategy was based on a modification of a conventional DNA-staining dye to suppress its affinity to the original targets, and subsequent re-selection of aptamers that would bind to the modified dye. Along this line, we prepared an environmental polarity-sensitive Hoechst derivative with low affinity to the usual AT-rich dsDNA targets. DNA aptamers, in vitro selected from a random pool, worked as a trigger to enhance the fluorescence of an otherwise nonfluorescent Hoechst derivative.
Narita
Atsushi
A
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Sando
Shinsuke
S
Aoyama
Yasuhiro
Y
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D006690:Bisbenzimidazole / Q000031:analogs & derivatives*; D004247:DNA / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D015342:DNA Probes; D005456:Fluorescent Dyes; D004247:DNA; D006690:Bisbenzimidazole