[Efficient packaging retrovirus and construction of transgenic chicken technical platform].
Transgenic chicken and oviduct bioreactor are growing to be one of the hotspot of scientific study in the field of biology. The most successful method of producing transgenic chicken is pseudotyped retrovirus vector system, but no one has reported the production of transgenic chicken by retrovirus system recently in our country. In order to accelerate our study in this field, we introduced the relevant technical methods such as packaging retrovirus and vesicular stomatitis virus G glycoprotein (VSV-G) pseudotyped retrovirus, optimizing the conditions of packaging retrovirus, concentrating VSV-G pseudotyped retrovirus, helper virus assays, and microinjection of retrovirus. Furthermore, we successfully conducted in vivo study for detecting the marker gene EGFP of chicken embryo as well as in vitro study for detecting that gene of chicken embryo myoblast (CFM), thus we have provided an applied technical platform for studies of transgenic chicken in the future.
Man
Chaolai
C
Department of Biotechnology, Harbin Institute of Technology, Harbin 150001, China.
Zhang
Qing
Q
Chen
Yan
Y
Zhu
Dahai
D
D000818:Animals; D030801:Animals, Genetically Modified; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D017931:DNA Primers; D005822:Genetic Vectors / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics*; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics
D017931:DNA Primers