title: Effects of ototopical antihistamine on otitis media in an allergic rat.
abstract: OBJECTIVE
  A reliable model of allergy has been created in the Brown Norway rat. In this model, allergen presentation to the middle ear causes functional disruption of the eustachian tube, predisposing to the development of otitis media with effusion. The purpose of this study was to study the effects of ototopical antihistamine on lipopolysaccharide (LPS)-induced otitis media in the allergic rat model.
  
  
  METHODS
  Prospective animal-based research study.
  
  
  METHODS
  Fifteen (n = 15) rats were made allergic via sensitization to ovalbumin (OVA) by subcutaneous injection and randomized into three groups: saline (SAL) + LPS, olopatadine (OLO) + LPS, and azelastine (AZE) + LPS. Allergic rats were transtympanically injected with OVA 24 hours prior to challenge, creating a subclinical inflammatory response in which there is no visible middle ear effusion. Thirty-five microliters of test substance (SAL + LPS, OLO + LPS, or AZE + LPS) were injected into the middle ear at 0, 2, and 4 hours. Effusion was collected at 2, 4, and 6 hours. Statistical analysis was performed on effusion volume and albumin concentration.
  
  
  RESULTS
  Significant increase in effusion volume with respect to time was noted for the SAL + LPS group, whereas the AZE + LPS group demonstrated a decrease. Intergroup comparison revealed a significant decrease in effusion volume at hour 6, with both AZE and OLO less than LPS alone. A significant decrease in albumin concentration over time was noted in the AZE group. Intergroup comparison revealed a significant difference inalbumin concentration at hour 6, with OLO significantly lower than LPS.
  
  
  CONCLUSIONS
  Ototopical antihistamines are effective in reducing effusion volume and albumin concentration in LPS-induced otitis media in an allergic rat model. This finding supports the hypothesis that ototopic antihistamines may prove effective in treating patients with allergy-induced eustachian tube dysfunction.
authors.affiliation: Department of Surgery, Section of Otolaryngology-Head and Neck Surgery, Walter Reed Army Medical Center, Washington, DC 20307, USA. Jeffrey.cutler@hotmail.com
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Cutler
authors.forename: Robert F
authors.initials: RF
authors.lastname: Labadie
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005512:Food Hypersensitivity / Q000517:prevention & control; D006634:Histamine H1 Antagonists / Q000494:pharmacology* / Q000627:therapeutic use*; D006967:Hypersensitivity / Q000150:complications* / Q000188:drug therapy*; D010034:Otitis Media with Effusion / Q000150:complications* / Q000188:drug therapy*; D011446:Prospective Studies; D051381:Rats
chemical_list: D006634:Histamine H1 Antagonists