title: [Simultaneous determination of dichlorvos, trichlorfon and naled in fruits and vegetables by liquid chromatography with tandem mass spectrometry].
abstract: A method for simultaneous determination of Dichlorvos (DDVP), Trichlorfon (DEP) and Naled (BRP) in fruits and vegetables by liquid chromatography with tandem mass spectrometry (LC/MS/MS) was developed. Pesticides were extracted with ethyl acetate together with phosphoric acid and anhydrous sodium sulfate, followed by an ENVI-Carb cartridge cleanup. Phosphoric acid prevented BRP from being converted to DDVP during extraction of pesticides from the sample. When the sample was dissolved in acetonitrile in a silanized glass vial, BRP and DEP remained intact. Mass spectral acquisition was performed with a TurbolonSpray (ESI) interface in the positive mode by applying multiple reaction monitoring. In LC separation, an ODS column was used with acetic acid-ammonium acetate-methanol as a mobile phase. Recoveries from 8 fruits and vegetables at the fortification level of 0.1 microg/g were 75.0-91.8% for BRP, 70.2-88.9% for DDVP, and 77.3-92.1% for DEP. The detection limits of BRP, DDVP and DEP were 1, 2 and 2 ng/g, respectively.
authors.affiliation: Nara Prefectural Institute for Hygiene and Environment, 57-6 Ohmori-cho, Nara-city, Nara 630-8131, Japan.
authors.forename: Sachiyo
authors.initials: S
authors.lastname: Ibuki
authors.forename: Katsushige
authors.initials: K
authors.lastname: Uranishi
authors.forename: Masakiyo
authors.initials: M
authors.lastname: Uno
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004006:Dichlorvos / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D007306:Insecticides / Q000032:analysis*; D009267:Naled / Q000032:analysis*; D010756:Phosphoric Acids / Q000494:pharmacology; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014236:Trichlorfon / Q000032:analysis*; D014675:Vegetables / Q000737:chemistry*
chemical_list: D007306:Insecticides; D010756:Phosphoric Acids; D004006:Dichlorvos; D014236:Trichlorfon; C030242:phosphoric acid; D009267:Naled