Improved method for the PCR-based gene disruption in Saccharomyces cerevisiae.
The PCR-based gene disruption strategy originally devised by Baudin et al. is widely used for gene targeting in Saccharomyces cerevisiae. An advantage of this strategy is its simplicity in making targeting constructs. The efficiencies of the targeted disruption are highly variable from locus to locus, however, and often very low. In this report, a method for improving the gene deletion efficiency is described.
Koyama
Hiroshi
H
Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo, Tokyo, Japan.
Sumiya
Eriko
E
Ito
Takahiro
T
Sekimizu
Kazuhisa
K
D018390:Gene Targeting / Q000379:methods; D016254:Mutagenesis, Insertional / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics*