Synthesis of peptide ribonucleic acid (PRNA)-DNA chimera and interaction with DNA and RNA.
Recently, we have demonstrated that effective control of the recognition behavior of peptide ribonucleic acid (PRNA) with complementary DNA is possible through the anti-to-synorientational change of pyrimidine nucleobase induced by borate ester formation. In this study, DNA-PRNA chimera was prepared by the solidphase synthesis. In the DNA-PRNA chimeras, both PRNA and DNA domains work as recognition sites for the complementary DNA/RNAs to form stable complex, while DNA-RNA hybrids formed in the DNA domains of DNA-PRNA chimera should be substrates to the hydrolysis by RNase H and PRNA moieties work as recognition control/switching devices and as inhibitor for the hydrolysis by exonucleases. Interaction of the DNA-PRNA chimera with DNA and RNA has been discussed.
Wada
Takehiko
T
Institute of Multidisciplinary Research for Advanced Materials,Tohoku University, 2-1-1, Katahira, Aobaku, Sendai 980-8577, Japan.
Maeda
Yoshiki
Y
Sawa
Nobuya
N
Sato
Hirofumi
H
Chon
Hyongi
H
Kanaya
Shigenori
S
Inoue
Yoshihisa
Y
D004247:DNA / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*
D020135:Peptide Nucleic Acids; D012313:RNA; D004247:DNA