title: The effect of a Glu370Asp mutation in glutaryl-CoA dehydrogenase on proton transfer to the dienolate intermediate.
abstract: We have determined steady-state rate constants and net rate constants for the chemical steps in the catalytic pathway catalyzed by the E370D mutant of glutaryl-CoA dehydrogenase and compared them with those of the wild-type dehydrogenase. We sought rationales for changes in these rate constants in the structure of the mutant cocrystallized with the alternate substrate, 4-nitrobutyric acid. Substitution of aspartate for E370, the catalytic base, results in a 24% decrease in the rate constant for proton abstraction at C-2 of 3-thiaglutaryl-CoA as the distance between C-2 of the ligand and the closest carboxyl oxygen at residue 370 increases from 2.9 A to 3.1 A. The net rate constant for flavin reduction due to hydride transfer from C-3 of the natural substrate, which includes proton abstraction at C-2, to N5 of the flavin decreases by 81% due to the mutation, although the distance increases only by 0.7 A. The intensities of charge-transfer bands associated with the enolate of 3-thiaglutaryl-CoA, the reductive half-reaction (reduced flavin with oxidized form of substrate), and the dienolate following decarboxylation are considerably diminished. Structural investigation suggests that the increased distance and the change in angle of the S-C1(=O)-C2 plane of the substrate with the isoalloxazine substantially alter rates of the reductive and oxidative half-reactions. This change in active site geometry also changes the position of protonation of the four carbon dienolate intermediate to produce kinetically favorable product, vinylacetyl-CoA, which is further isomerized to the thermodynamically stable normal product, crotonyl-CoA.
authors.affiliation: Department of Pediatrics, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado 80045-0511, USA.
authors.forename: K Sudhindra
authors.initials: KS
authors.lastname: Rao
authors.forename: Zhuji
authors.initials: Z
authors.lastname: Fu
authors.forename: Mark
authors.initials: M
authors.lastname: Albro
authors.forename: Beena
authors.initials: B
authors.lastname: Narayanan
authors.forename: Saritha
authors.initials: S
authors.lastname: Baddam
authors.forename: Hyun-Joo K
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jung-Ja P
authors.initials: JJ
authors.lastname: Kim
authors.forename: Frank E
authors.initials: FE
authors.lastname: Frerman
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D019943:Amino Acid Substitution; D001224:Aspartic Acid / Q000235:genetics / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003461:Crystallography; D018698:Glutamic Acid / Q000235:genetics / Q000378:metabolism; D050770:Glutaryl-CoA Dehydrogenase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D013058:Mass Spectrometry; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D011522:Protons; D013379:Substrate Specificity
chemical_list: D000214:Acyl Coenzyme A; D011522:Protons; D001224:Aspartic Acid; C015901:glutaryl-coenzyme A; D018698:Glutamic Acid; D050770:Glutaryl-CoA Dehydrogenase