A new mRNA splice variant coding for the human EP3-I receptor isoform.
The cellular localization of prostaglandin E2 receptors (EP) and their corresponding transcripts were investigated in human gastric and vascular tissues. A strong staining of the EP3 receptor on the gastric glands, mucous cells, media of the mammary and pulmonary arteries was observed by immunohistochemistry. We identified a new mRNA splice variant of the EP3 gene in human gastric fundic mucosa, mammary artery and pulmonary vessels. This EP3-Ic transcript contains exons 1, 2, 3, 5 and 6 of the EP3 gene and should be translated in the EP3-I isoform. In addition, the EP3-Ib, EP3-II, EP3-III, EP3-IV and EP3-e mRNAs were detected in these tissues.
Kotelevets
L
L
CHU X-Bichat, Paris F-75018, France.
Foudi
N
N
Louedec
L
L
Couvelard
A
A
Chastre
E
E
Norel
X
X
D001483:Base Sequence; D005260:Female; D005748:Gastric Fundus / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008323:Mammary Arteries / Q000378:metabolism; D008875:Middle Aged; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics*; D011651:Pulmonary Artery / Q000378:metabolism; D011667:Pulmonary Veins / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D018078:Receptors, Prostaglandin E / Q000235:genetics*; D058308:Receptors, Prostaglandin E, EP3 Subtype; D020133:Reverse Transcriptase Polymerase Chain Reaction
C545647:PTGER3 protein, human; D020033:Protein Isoforms; D012333:RNA, Messenger; D018078:Receptors, Prostaglandin E; D058308:Receptors, Prostaglandin E, EP3 Subtype