Induction and preliminary characterization of a novel halophage SNJ1 from lysogenic Natrinema sp. F5.
Halophage SNJ1 was induced with mitomycin C from Natrinema sp. strain F5. The phage produces plaques on Natrinema sp. strain J7 only. The phage has a head of about 67 nm in diameter and a tail of 570 nm in length and belongs morphologically to the family Siphoviridae. The phage is strongly salt dependent; NaCl concentration affects the integrity of SNJ1, phage adsorption, and plaque formation. The optimal NaCl concentration for phage adsorption and plaque formation is 30% and 25%, respectively.
Mei
Yunjun
Y
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Chen
Jin
J
Sun
Dongchang
D
Chen
Dong
D
Yang
Yang
Y
Shen
Ping
P
Chen
Xiangdong
X
D023641:Archaeal Viruses / Q000145:classification / Q000502:physiology / Q000648:ultrastructure; D006216:Halobacteriaceae / Q000821:virology*; D008242:Lysogeny / Q000502:physiology*; D016685:Mitomycin / Q000494:pharmacology; D017906:Siphoviridae / Q000145:classification / Q000502:physiology / Q000648:ultrastructure; D012965:Sodium Chloride / Q000494:pharmacology; D010948:Viral Plaque Assay; D014775:Virus Activation / Q000502:physiology*
D012965:Sodium Chloride; D016685:Mitomycin