Interactions with RNA/DNA of proteins involved in the regulation of transcription, translation and telomere elongation.
Interactions with DNA and RNA of three different proteins involved in the regulation of (1) transcription, (2) translation, and (3) telomere elongation were examined by NMR. In the first case, the combination of structural determination, dynamical analysis on the basis of relaxation data and identification of interactive surface for wild and phosphorylation-mimicking mutant proteins has given the insight on the increase of DNA-binding affinity through phosphorylation of the protein. In the second case, the arrangement of two tandem domains interacting with RNA has been determined with residual dipolar couplings and paramagnetic relaxation enhancement, which has given the idea on how the two tandem domains recognize the target RNA. In the third case, simultaneous binding of the other two tandem domains to both DNA and RNA has been analyzed with chemical shift perturbation analysis. The result has suggested that the protein composed of two tandem domains can recruit telomerase to telomere DNA.
Ohyama
Takako
T
Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
Furukawa
Ayako
A
Miyoshi
Tatsuya
T
Takada
Yuusuke
Y
Ohgara
Shouta
S
Hiratsuka
Kazuyuki
K
Imai
Takao
T
Okano
Hideyuki
H
Nakagama
Hitoshi
H
Nagata
Takashi
T
Katahira
Masato
M
D004247:DNA / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry*; D005786:Gene Expression Regulation; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D012313:RNA / Q000737:chemistry; D016601:RNA-Binding Proteins / Q000737:chemistry*; D016615:Telomere / Q000737:chemistry; D014157:Transcription Factors / Q000737:chemistry*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; C077926:GT-1 transcription factor; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D016601:RNA-Binding Proteins; D014157:Transcription Factors; D012313:RNA; D004247:DNA