title: Structural and physicochemical features on the metal-mediated base pairs.
abstract: The chemical structure of the mercury-mediated T-T pair (T-Hg(I)I-T) was determined with (15)N NMR spectroscopy. In order to determine the chemical structure of the T-Hg(I)I-T pair, (15)N-(15)N J-coupling across a metal center (2JNN) was employed. Notably, this is the first observation of (2)J(NN) in a biological macromolecule (DNA duplex). This pairing mode was found to be a irregular metal ion-binding mode for DNA and RNA molecules, in which imino proton-metal exchange processes are included. Accordingly, (2)J(NN) is highly important for the determination of the chemical structures of metal-mediated base pairs.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-8578, Japan. tanaka@mail.pharm.tohoku.ac.jp
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Shuji
authors.initials: S
authors.lastname: Oda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Kaichiro
authors.initials: K
authors.lastname: Haruta
authors.forename: Takuya
authors.initials: T
authors.lastname: Kawamura
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kondo
authors.forename: Tomomi
authors.initials: T
authors.lastname: Uchiyama
authors.forename: Miura
authors.initials: M
authors.lastname: Takashi
authors.forename: Hideo
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Torigoe
authors.forename: Chojiro
authors.initials: C
authors.lastname: Kojima
authors.forename: Akira
authors.initials: A
authors.lastname: Ono
mesh_terms: D020029:Base Pairing; D004247:DNA / Q000737:chemistry; D008628:Mercury / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D013941:Thymine / Q000737:chemistry*
chemical_list: D004247:DNA; D008628:Mercury; D013941:Thymine