title: Plasticity of epithelial cells derived from human normal and ADPKD kidneys in primary cultures.
abstract: Autosomal dominant polycystic kidney disease (ADPKD) is characterized by cyst formation initiated by dedifferentiation and proliferation of renal tubular epithelial cells. Renal tubular epithelial cells (RTC, derived from normal kidney tissue) in primary cultures exhibit both homogeneous expression of gamma-glutamyl transferase and low molecular weight cytokeratin, two different markers for proximal and distal renal epithelial cells, respectively. RTC in cultures also abnormally express the dedifferentiation markers vimentin and PAX-2, which are proteins normally expressed in epithelial cells lining cysts in ADPKD kidneys but not tubular cells in normal kidneys. In contrast, different cultures of cystic epithelial cells (CEC, derived from the cysts walls of polycystic kidneys) display variable expression of cytokeratin, gamma-glutamyl transferase, and PAX-2, but a constant level of vimentin. Importantly, RTC and CEC exhibit the capacity to convert to their respective original structures by forming tubules and cysts, respectively, when cultured in a three-dimensional gel matrix, whereas HK-2, LLC-PK1, and MDCK renal epithelial cell lines form cell aggregates or cysts. Our study demonstrates that the marker expression of the various epithelial cell types is not highly stable in primary cultures. Their modulation is different in cells originating from normal and ADPKD kidneys and in cells cultured in monolayer and three-dimensions. These results indicate the plasticity of epithelial cells that display a mixed epithelial/dedifferentiated/mesenchymal phenotype during their expansion in culture. However, RTC and CEC morphogenic epithelial properties in three-dimensional cultures are similar to those in vivo. Thus, this model is useful for studying the mechanisms leading to tubulogenesis and cystogenesis.
authors.affiliation: Department of Pediatrics, Section of Nephrology, The University of Oklahoma Health Sciences Center, 940 N.E.13th Street, 2B2309, Oklahoma City, OK 73104, USA. Gerard-elberg@ouhsc.edu
authors.forename: Gerard
authors.initials: G
authors.lastname: Elberg
authors.forename: Suresh
authors.initials: S
authors.lastname: Guruswamy
authors.forename: Charlotte J
authors.initials: CJ
authors.lastname: Logan
authors.forename: Lijuan
authors.initials: L
authors.lastname: Chen
authors.forename: Martin A
authors.initials: MA
authors.lastname: Turman
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D002923:Cilia / Q000378:metabolism / Q000648:ultrastructure; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology* / Q000378:metabolism; D016891:Polycystic Kidney, Autosomal Dominant / Q000473:pathology*; D015203:Reproducibility of Results; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: D015415:Biomarkers; D005723:gamma-Glutamyltransferase