Short-step chemical synthesis of DNA by use of MMTrS group for protection of 5'-hydroxyl group.
4-methoxytrithylthio (MMTrS) group was applied for the appropriately protected four canonical nucleosides. We prepared the phosphoroamidite units by use of these nucleosides and developed the synthesis of oligodeoxynucleotides without any acidic treatment. Moreover, the new DNA synthesis protocol was applied to an automated DNA synthesizer for the synthesis of longer oligodeoxynucleotides.
Shiraishi
Miyuki
M
Department of life science, Tokyo Institute of Tecnology, Nagatsuta, Midoriku, Yokohama, 226-8501, Japan.
Utagawa
Eri
E
Ohkubo
Akihiro
A
Sekine
Mitsuo
M
Seio
Kohji
K
D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D014320:Triphenylmethyl Compounds / Q000737:chemistry*
C528348:4-methoxytritylthio chloride; D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; D014320:Triphenylmethyl Compounds; C434331:phosphoramidite