Purification, crystallization and initial crystallographic characterization of brazil-nut allergen Ber e 2.
Peanut and tree-nut allergies have attracted considerable attention because of their frequency and their lifelong persistence. Brazil-nut (Bertholletia excelsa) allergies have been well documented and the 11S legumin-like seed storage protein Ber e 2 (excelsin) is one of the two known brazil-nut allergens. In this study, Ber e 2 was extracted from brazil-nut kernels and purified to high purity by crystalline precipitation and gel-filtration chromatography. Well diffracting single crystals were obtained using the hanging-drop vapour-diffusion method. A molecular-replacement structural solution has been obtained. Refinement of the structure is currently under way.
Guo
Feng
F
Department of Biology, Illinois Institute of Technology, Chicago, IL 60616, USA.
Jin
Tengchuan
T
Howard
Andrew
A
Zhang
Yu Zhu
YZ
D000485:Allergens / Q000737:chemistry* / Q000302:isolation & purification*; D052179:Antigens, Plant / Q000737:chemistry* / Q000302:isolation & purification*; D031383:Bertholletia / Q000737:chemistry*; D002850:Chromatography, Gel; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D012995:Solubility
D000485:Allergens; D052179:Antigens, Plant; C525128:Ber e 2 protein, Bertholletia excelsa