title: Lipid mapping in human dystrophic muscle by cluster-time-of-flight secondary ion mass spectrometry imaging.
abstract: Human striated muscle samples, from male control and Duchenne muscular dystrophy-affected children, were subjected to cluster-time-of-flight secondary ion mass spectrometry (cluster-ToF-SIMS) imaging using a 25 keV Bi(3)(+) liquid metal ion gun under static SIMS conditions. Spectra and ion density maps, or secondary ion images, were acquired in both positive and negative ion mode over several areas of 500 x 500 microm(2) (image resolution, 256 x 256 pixels). Characteristic distributions of various lipids were observed. Vitamin E and phosphatidylinositols were found to concentrate within the cells, whereas intact phosphocholines accumulated over the most damaged areas of the dystrophic muscles, together with cholesterol and sphingomyelin species. Fatty acyl chain composition varied depending on the region, allowing estimation of the local damage extent.
authors.affiliation: Laboratoire de Spectrométrie de Masse, Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2301, Gif sur Yvette Cedex, France.
authors.forename: Nora
authors.initials: N
authors.lastname: Tahallah
authors.forename: Alain
authors.initials: A
authors.lastname: Brunelle
authors.forename: Sabine
authors.initials: S
authors.lastname: De La Porte
authors.forename: Olivier
authors.initials: O
authors.lastname: Laprévote
mesh_terms: D000293:Adolescent; D000818:Animals; D002648:Child; D004195:Disease Models, Animal; D006801:Humans; D008055:Lipids / Q000032:analysis / Q000737:chemistry*; D008297:Male; D051379:Mice; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology*; D020388:Muscular Dystrophy, Duchenne / Q000378:metabolism* / Q000473:pathology; D018629:Spectrometry, Mass, Secondary Ion
chemical_list: D008055:Lipids