title: [Cyclophosphamide-induced rat ovarian damage and expression of gonadotropin-releasing hormone receptor in the damaged ovaries].
abstract: OBJECTIVE
  To investigate ovarian follicular damage induced by chemotherapeutic agents and gonadotropin- releasing hormone receptor (GnRHR) expression in the damaged ovaries in rats.
  
  
  METHODS
  Two groups of adult SD rats were subjected to intraperitoneal injection of a single-dose cyclophosphamide and saline, respectively, and 8 weeks later, the ovaries were taken for observing the ovarian damages. The distribution of GnRHR was detected with immunohistochemistry, and RT-PCR was used to determine the expression of GnRHR mRNA in the rat ovaries.
  
  
  RESULTS
  Massive primordial follicular loss occurred in the ovaries of rats exposed to cyclophosphamide with also evident stromal ovarian blood vessel damages and focal fibrosis. Both the protein and mRNA expressions of GnRHR were detected in normal rat ovaries, but in rats exposed to cyclophosphamide, the expressions were significantly lowered in the ovaries (P<0.05).
  
  
  CONCLUSIONS
  Low-level GnRHR expressions in the ovaries of rats with cyclophosphamide exposure suggest microenvironment disturbances in the damaged rat ovaries in advanced stage of chemotherapy.
authors.affiliation: Department Obstetrics and Gynecology, Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China. jessicaluolu@163.com
authors.forename: Lu
authors.initials: L
authors.lastname: Luo
authors.forename: Dong-zi
authors.initials: DZ
authors.lastname: Yang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Ya-qin
authors.initials: YQ
authors.lastname: Mo
authors.forename: Qing-xue
authors.initials: QX
authors.lastname: Zhang
authors.forename: Cheng-yu
authors.initials: CY
authors.lastname: Zheng
mesh_terms: D000818:Animals; D003520:Cyclophosphamide / Q000009:adverse effects*; D005260:Female; D006801:Humans; D010053:Ovary / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011966:Receptors, LHRH / Q000378:metabolism*
chemical_list: D011966:Receptors, LHRH; D003520:Cyclophosphamide