title: Novel gel-based rapid test for non-instrumental detection of ochratoxin A in beer.
abstract: A rapid easy-to-use immunoassay was optimised for the non-instrumental detection of ochratoxin A (OTA) in beer. The analytical method involves preconcentration on the immunoaffinity layer inside a column followed by direct competitive ELISA detection in the same layer. The visual cut-off value, i.e. the lowest OTA concentration resulting in no colour development, was 0.2 microg L(-1). Assay validation was performed using samples spiked with OTA. Thirty-seven naturally contaminated samples were screened with the gel-based method developed and no false-negative results were obtained. The method described offers a simple, rapid and cost-effective screening tool, thus contributing to better health protection of consumers.
authors.affiliation: Department of Common and Inorganic Chemistry, Chemistry Faculty, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia. goryachevaiy@info.sgu.ru
authors.forename: I Y
authors.initials: IY
authors.lastname: Goryacheva
authors.forename: E Y
authors.initials: EY
authors.lastname: Basova
authors.forename: C
authors.initials: C
authors.lastname: Van Peteghem
authors.forename: S A
authors.initials: SA
authors.lastname: Eremin
authors.forename: L
authors.initials: L
authors.lastname: Pussemier
authors.forename: J-C
authors.initials: JC
authors.lastname: Motte
authors.forename: S
authors.initials: S
authors.lastname: De Saeger
mesh_terms: D001515:Beer / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination; D005782:Gels / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D009793:Ochratoxins / Q000032:analysis*; D013997:Time Factors
chemical_list: D005782:Gels; D009793:Ochratoxins; C025589:ochratoxin A