title: Evidence for a new species of Leishmania associated with a focal disease outbreak in Ghana.
abstract: A previously unknown Leishmania spp., inferred by DNA sequence analysis of the small subunit ribosomal RNA gene and the internal transcribed spacer region (ITS1), was detected in tissue biopsies from patients living in the Eastern Ghanaian community of Taviefe. Restriction fragment length polymorphism analysis of the ITS1 amplicon supports the possibility of an uncharacterized Leishmania spp.
authors.affiliation: US Naval Medical Research Unit No. 3, Cairo, Egypt.
authors.forename: Jeffrey T
authors.initials: JT
authors.lastname: Villinski
authors.forename: John D
authors.initials: JD
authors.lastname: Klena
authors.forename: Magda
authors.initials: M
authors.lastname: Abbassy
authors.forename: David F
authors.initials: DF
authors.lastname: Hoel
authors.forename: Naiki
authors.initials: N
authors.lastname: Puplampu
authors.forename: Sarah
authors.initials: S
authors.lastname: Mechta
authors.forename: Daniel
authors.initials: D
authors.lastname: Boakye
authors.forename: Gregory
authors.initials: G
authors.lastname: Raczniak
mesh_terms: D000818:Animals; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks; D005869:Ghana / Q000453:epidemiology; D006801:Humans; D007891:Leishmania / Q000145:classification* / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000453:epidemiology* / Q000469:parasitology*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D021901:DNA, Intergenic; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S