title: Isolation and characterization of a new facultatively autotrophic hydrogen-oxidizing Betaproteobacterium, Hydrogenophaga sp. AH-24.
abstract: A hydrogen-oxidizing bacterium strain AH-24 was isolated, which was classified in the genus Hydrogenophaga, based on the 16S rRNA gene sequence. The isolate possessed a typical yellow pigment of Hydrogenophaga species. Its closest relative was Hydrogenophaga pseudoflava, but the assimilation profile of sugar compounds resembled that of no species of Hydrogenophaga. The optimum temperature and pH for autotrophic growth were, respectively, 33-35 degrees C and 7.0. Most hydrogenase activity (benzyl viologen reducing activity) was localized in the membrane fraction (MF), but NAD(P)-reducing hydrogenase activity was detected in neither the membrane nor the soluble fractions. Cytochromes b561 and c551 were present in MF; both were reduced when hydrogen was supplied to the oxidized MF, suggesting involvement in respiratory H2 oxidation as electron carriers. Cytochrome b561 was inferred to function as the redox partner of the membrane-bound hydrogenase.
authors.affiliation: Department of Bioresource Science, School of Agriculture, Ibaraki University, Inashiki-gun, Ibaraki, Japan.
authors.forename: Ki-Seok
authors.initials: KS
authors.lastname: Yoon
authors.forename: Natsuki
authors.initials: N
authors.lastname: Tsukada
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Sakai
authors.forename: Masaharu
authors.initials: M
authors.lastname: Ishii
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Igarashi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Nishihara
mesh_terms: D002462:Cell Membrane / Q000737:chemistry; D042621:Comamonadaceae / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D003580:Cytochromes / Q000032:analysis / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006859:Hydrogen / Q000378:metabolism*; D006864:Hydrogenase / Q000032:analysis / Q000378:metabolism; D000070116:Lotus; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013057:Spectrum Analysis
chemical_list: D003580:Cytochromes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D006859:Hydrogen; D006864:Hydrogenase