Use of 120-nm deep channels for liquid chromatographic separations.
The present study reports on the exploration of the separation speed limits of RPLC chromatography in open-tubular channels. Applying the shear-driven chromatography principle in a 120-nm deep open channel, and using an improved detection set-up, the separation of three coumarin dyes was detectable 8mm downstream of the injection point. At this distance, separation efficiencies of N = 17,900 - 24,100 plates were obtained at a velocity of 10 mm s(-1), corresponding to a plate generation velocity of 21,100 to 28,300 plates per second for the most and least retained component, respectively.
Fekete
Veronika
V
Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium.
Clicq
David
D
De Malsche
Wim
W
Gardeniers
Han
H
Desmet
Gert
G
D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D008962:Models, Theoretical