Assessment of 17alpha-ethinylestradiol effects and underlying mechanisms in a continuous, multigeneration exposure of the Chinese rare minnow (Gobiocypris rarus).
17alpha-Ethinylestradiol (EE(2)) is a synthetic estrogen used primarily in birth control pills and in hormone replacement therapy. Owing to its occurrence in surface waters at concentrations frequently greater than 1 ng/l and its projected future use, EE(2) is expected to pose a significant risk to aquatic organisms. This study was conducted to obtain long-term exposure data necessary for the establishment of water quality criteria and to investigate mechanisms associated with toxic effects. In a multigeneration experiment, Chinese rare minnows (Gobiocypris rarus) were constantly exposed to environmentally relevant concentrations of the synthetic estrogen EE(2). Mortality, deformities, reproductive parameters, plasma vitellogenin and histopathology were assessed. The results showed that, in the F(0) generation, all endpoints were significantly affected at concentrations higher than 0.2 ng/l EE(2). No F(1) phenotypic males developed to maturity at 0.2 ng/l and, when adult females of this exposure group were crossed with unexposed males, no F(2) fertile eggs were produced. Kidney histopathology and ultrastructure suggest anomalies possibly associated with increased vitellogenin accumulation. We concluded that the reproduction of the F(1) minnows was completely inhibited at the lowest concentration tested, 0.2 ng/l EE(2), a concentration frequently detected in surface waters. Growth effects may be related to increased energy requirements including the energy used in VTG synthesis. Reproductive effects are presumably associated with male feminization and the occurrence of testis-ova in males; however, ovarian degeneration observed in females may also have contributed to reproductive failure.
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Shuangqing Rd 18, Haidian District, Beijing, 100085, PR China.
Jinmiao
J
Zha
Liwei
L
Sun
Yiqi
Y
Zhou
Philip A
PA
Spear
Mei
M
Ma
Zijian
Z
Wang
D000014:Abnormalities, Drug-Induced; D000818:Animals; D003530:Cyprinidae; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000002:abnormalities / Q000187:drug effects; D004967:Estrogens / Q000633:toxicity*; D004997:Ethinyl Estradiol / Q000633:toxicity*; D005260:Female; D005262:Feminization / Q000139:chemically induced / Q000473:pathology; D022781:Hepatocytes / Q000187:drug effects / Q000648:ultrastructure; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008018:Life Cycle Stages / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008136:Longevity / Q000187:drug effects; D008297:Male; D010053:Ovary / Q000187:drug effects / Q000473:pathology; D012098:Reproduction / Q000187:drug effects*; D013737:Testis / Q000187:drug effects / Q000473:pathology; D018675:Toxicity Tests; D014819:Vitellogenins / Q000097:blood
D004967:Estrogens; D014819:Vitellogenins; D004997:Ethinyl Estradiol