An enhanced miniaturized assay for antimicrobial prospecting.
Bioprospecting for novel antimicrobials increasingly relies on extremely small samples unsuitable for conventional bulk extraction and assay. We developed a microtitre plate assay for minimal amounts of test materials which is rapid, extremely sensitive, allows time-course analysis and reduces false negatives. Developed for the analyses of antimicrobial sensitivity and resistance, the technique is appropriate for assays where source materials are scarce.
Department of Biological Sciences, Macquarie University, NSW, Australia. smsmith@bio.mq.edu.au
Shannon M
SM
Smith
Andrew J
AJ
Beattie
Michael R
MR
Gillings
Marita P
MP
Holley
Adam J
AJ
Stow
Christine L
CL
Turnbull
Peter D
PD
Wilson
David A
DA
Briscoe
D000667:Ampicillin / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D020128:Inhibitory Concentration 50; D007313:Insecta / Q000737:chemistry; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010957:Plasmids; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013307:Streptomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D000667:Ampicillin; D013307:Streptomycin