Assessment of lipopolysaccharide-binding activity of Bifidobacterium and its relationship with cell surface hydrophobicity, autoaggregation, and inhibition of interleukin-8 production.
This study was performed to screen probiotic bifidobacteria for their ability to bind and neutralize lipopolysaccharides (LPS) from Escherichia coli and to verify the relationship between LPS-binding ability, cell surface hydrophobicity (CSH), and inhibition of LPS-induced interleukin-8 (IL-8) secretion by HT-29 cells of the various bifidobacterial strains. Ninety bifidobacteria isolates from human feces were assessed for their ability to bind fluorescein isothiocyanate (FITC)-labeled LPS from E. coli. Isolates showing 30-60% binding were designated LPS-high binding (LPS-H) and those with less than 15% binding were designated LPS-low binding (LPS-L). The CSH, autoaggregation (AA), and inhibition of LPS-induced IL-8 release from HT-29 cells of the LPS-H and LPS-L groups were evaluated. Five bifidobacteria strains showed high levels of LPS binding, CSH, AA, and inhibition of IL-8 release. However, statistically significant correlations between LPS binding, CSH, AA, and reduction of IL-8 release were not found. Although we could isolate bifidobacteria with high LPS-binding ability, CSH, AA, and inhibition of IL-8 release, each characteristic should be considered as strain dependent. Bifidobacteria with high LPS binding and inhibition of IL-8 release may be good agents for preventing inflammation by neutralizing Gram-negative endotoxins and improving intestinal health.
Park
Myeong Soo
MS
Research Institute, BIFIDO Co., Ltd., Hongchun 250-804, Korea.
Kim
Min Jeong
MJ
Ji
Geun Eog
GE
D001644:Bifidobacterium / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology*; D004926:Escherichia coli / Q000737:chemistry / Q000145:classification; D005243:Feces / Q000382:microbiology; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D016209:Interleukin-8 / Q000037:antagonists & inhibitors*; D008070:Lipopolysaccharides / Q000378:metabolism*; D008565:Membrane Proteins / Q000502:physiology; D019936:Probiotics / Q000494:pharmacology; D012703:Serotyping; D013499:Surface Properties
D005456:Fluorescent Dyes; D016209:Interleukin-8; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D016650:Fluorescein-5-isothiocyanate