Analysis of CoQ10 in rat serum by ultra-performance liquid chromatography mass spectrometry after oral administration.
A UPLC-MS method for determining Coenzyme Q(10) (CoQ(10)) levels in rat serum was developed. CoQ(10) was quantitatively extracted into 2-propanol using a fast extraction procedure. The separation of CoQ(10) was performed on a Waters Acquity UPLCtrade mark BEH C(18) column (1.7 microm, 1.0 mm x 50 mm) with the mobile phase containing acetonitrile, 2-propanol, and formic acid (90:10:0.1) over 5 min. The sensitivity of this method allows for the quantitation of 50 ng/mL CoQ(10) in serum (S/N=10). The linearity of this method was found to be from 50 to 20,000 ng/mL. The precision was less than 10% (intra- and inter-day), and the average extraction recovery was between 90 and 105%. This procedure provides a precise, sensitive and direct assay method for the determination of CoQ(10) in rat serum after oral administration. This method could be applied to further pharmacokinetic studies of CoQ(10).
Li
Lie
L
Department of Pharmaceutics, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
Pabbisetty
Deepthi
D
Carvalho
Paulo
P
Avery
Mitchell A
MA
Avery
Bonnie A
BA
D019840:2-Propanol / Q000737:chemistry; D000085:Acetates / Q000737:chemistry; D019342:Acetic Acid / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000284:Administration, Oral; D064753:Ammonium Hydroxide; D000818:Animals; D019540:Area Under Curve; D002853:Chromatography, Liquid / Q000379:methods*; D003067:Coenzymes / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics; D005561:Formates / Q000737:chemistry; D006878:Hydroxides / Q000737:chemistry; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D008657:Metabolic Clearance Rate; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics / Q000592:standards
D000085:Acetates; D000097:Acetonitriles; D003067:Coenzymes; D005561:Formates; D006878:Hydroxides; D012997:Solvents; C030544:formic acid; D014451:Ubiquinone; D064753:Ammonium Hydroxide; C024989:coenzyme Q10; C030571:ubiquinone 9; D019840:2-Propanol; D019342:Acetic Acid; C018824:ammonium acetate; C032159:acetonitrile