Optimization of tannase production by Aureobasidium pullulans DBS66.
Tannase production by Aureobasidium pullulans DBS66 was optimized. The organism produced maximum tannase in the presence of 1% tannic acid after 36 h. Maximum gallic acid accumulation was observed within 36 h and tannic acid in the fermented broth was completely degraded after 42 h of growth. Glucose had a stimulatory effect on tannase synthesis at 0.1% (w/v) concentration. The organism showed maximum tannase production with (NH4)2HPO4 as nitrogen source. Shaking speed of 120 rpm and 50-ml broth volume have been found to be suitable for maximum tannase production.
Department of Microbiology, Vidyasagar University, Midnapore-721102, West Bengal, India. debu33@yahoo.com
Debdulal
D
Banerjee
Bikas R
BR
Pati
D001203:Ascomycota / Q000201:enzymology* / Q000254:growth & development; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D005707:Gallic Acid / Q000378:metabolism; D013634:Tannins / Q000378:metabolism
D013634:Tannins; D005707:Gallic Acid; D002265:Carboxylic Ester Hydrolases; C022711:tannase