[Study of target pegylated recombinant mutant human granulocyte colony stimulating factor].
Recombinant mutant human granulocyte colony stimulating factor (rmhG-CSF) was pegylated, purified and characterized. rhG-CSF was mutated in position 1,3,4,5,17, and cysteine was added in C-terminal. rmhG-CSF was pegylated by PEG-Mal 20000 and separated by ion-exchange chromatography, gel filtration chromatography. Analysis of SDS-PAGE showed thar the purity of the separated PEG-rmhG-CSF was greater than 95%. and in intro and in vivo bioactivity study showed that target modified PEG-rmhG-CSF kept full bioactivity which was better than traditional pegylation method, and longer half-life was proved in mice.
Institute of Infectious Diseases, First Affiliated Hospital of Medical College, Zhejiang University, Hang Zhou 310003, China.
Yan-Shan
YS
Huang
Rong
R
Jin
Xiao-Ni
XN
Liu
Chang-Mei
CM
Wang
Ya-Wen
YW
Rong
Zhi
Z
Chen
D000595:Amino Acid Sequence; D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000096:biosynthesis / Q000235:genetics*; D011092:Polyethylene Glycols / Q000737:chemistry*; D021382:Protein Sorting Signals; D011994:Recombinant Proteins
D050505:Mutant Proteins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D011092:Polyethylene Glycols