[Effect of seleno-arginine on cellular immunological function in D-gal aging mice].
OBJECTIVE
To study the effect of seleno-argnine on cellular immunological function in D-gal aging mice.


METHODS
The mice were divided into normal group, model group, low seleno-argnine group(L-SeArg) and high seleno-argnine group (H-SeArg). D-galactose induced aging model was set up by s.c. injection for 42 days, i.g. method was used to add Seleno-argnine. Spleen index(SI) and thymus index (TI) were measured. The ability of Spleen cell multiplication and NK cell killing activity were determined by MTT colorimetry. The changes of CD40 and CD40L expression were observed by immunofluorescent assay.


RESULTS
Compared with the model mice, seleno-argnine increased spleen index and thynus index, enhanced the ability of spleen cell multiplication, enhanced the expression of CD40 and CD40L, and accelerated the NK cell killing activity. Between the two seleno-argnine groups, the effect of L-SeArg on cellular immunological function was more obvious in D-gal aging mice.The result were statistic ally significantly compared with the model mice(P<0.01).


CONCLUSIONS
D-gal injection can decrease the cellular immunological function of mice. Seleno-argnine can activate immune cells, enhance the cellular immunological function of mice, reduce the toxic effect of D-gal and delay the aging process.
Ma
Yan-Hong
YH
College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China. ma_yhhyy@126.com
Liu
An-Jun
AJ
Zhang
Guo-Rong
GR
Lang
Jing
J
D000375:Aging / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D001120:Arginine / Q000737:chemistry / Q000494:pharmacology*; D019013:CD40 Antigens / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D019587:Dietary Supplements; D005260:Female; D005455:Fluorescent Antibody Technique; D005690:Galactose / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000276:immunology; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D012643:Selenium / Q000737:chemistry / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology
D019013:CD40 Antigens; D001120:Arginine; D012643:Selenium; D005690:Galactose