A soluble and active form of Wnt-3a protein is involved in myogenic differentiation after cholesterol depletion.
Cholesterol is one of the major lipids of plasma membranes. Recently, we have shown that cholesterol depletion by methyl-beta-cyclodextrin (M beta CD) induces the activation of the Wnt/beta-catenin pathway and enhances myogenic differentiation. Here, we show that M beta CD-conditioned media accelerates myogenesis in a similar way as M beta CD does, suggesting that the effects induced by M beta CD could be caused by soluble factors present in the culture medium. Soluble Wnt-3 protein is significantly enhanced in M beta CD-conditioned medium. Wnt-3a-enriched media induces myogenesis as much as M beta CD does, whereas Wnt-5a-enriched media inhibits. We suggest that Wnt-3a is involved in the myogenic induction observed after cholesterol depletion.
Laboratório de Diferenciação Muscular e Citoesqueleto, Departamento de Histologia e Embriologia, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21949-590, Brazil.
Débora M
DM
Portilho
Eliane R
ER
Martins
Manoel L
ML
Costa
Cláudia S
CS
Mermelstein
D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002459:Cell Fusion; D002478:Cells, Cultured; D002642:Chick Embryo; D002784:Cholesterol / Q000172:deficiency*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008970:Molecular Weight; D032342:Muscle Cells / Q000166:cytology* / Q000187:drug effects; D024510:Muscle Development / Q000187:drug effects; D051381:Rats; D012995:Solubility / Q000187:drug effects; D051153:Wnt Proteins / Q000378:metabolism*; D060508:Wnt3 Protein; D060509:Wnt3A Protein; D047392:beta-Cyclodextrins / Q000494:pharmacology; D000099274:Frizzled-Related Proteins
D015820:Cadherins; D002784:Cholesterol; D017077:Culture Media, Conditioned; D006023:Glycoproteins; D047908:Intracellular Signaling Peptides and Proteins; D051153:Wnt Proteins; D060508:Wnt3 Protein; D060509:Wnt3A Protein; D047392:beta-Cyclodextrins; D000099274:Frizzled-Related Proteins; C555507:WNT3 protein, human; C555510:WNT3A protein, human; C031215:betadex