GPR26: a marker for primary glioblastoma?
Glioblastomas are highly malignant brain tumours; they have been described as one of the most deadly human cancers. Two conceptual classifications of the condition exist: primary (de novo), which does not exhibit prior disease and secondary glioblastoma, which develops from a pre-existing glioma. This study investigates whether GPR26 is differentially transcribed in glioblastoma tissue from patients of different ages, in order to define a candidate genetic marker. The transcriptional profile of GPR26 was compared in nine samples: seven glioblastoma tissues and two normal brain tissues using PCR. Despite GPR26 being present in the glioblastoma tissues, it was not transcribed in any of the four cell lines tested. GPR26 transcription ratios were compared between normal and cancerous samples, also age categories <50 and >60 years were compared. Results suggested differential transcription of GPR26, which is significantly less transcribed in tissues from older patients, implied by a p-value of 0.03. This study has identified GPR26 to be a genetic indicator of primary glioblastoma, suggesting that it could be a suppressor of primary glioblastoma development.
Carter
Alan N
AN
Brain Tumour North West, Faculty of Science, University of Central Lancashire, Preston, UK. aashervington@uclan.ac.uk
Cole
Clare L
CL
Playle
Adam G
AG
Ramsay
Emma J
EJ
Shervington
Amal A
AA
D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D018628:Gene Dosage; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; C489011:GPR26 protein, human; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled