[Changes of physiological and biochemical characteristics of rat erythrocytes after blood loss].
In experiments of Wistar male rats, changes are studied of erythrocyte hematological, biochemical (activitities of transport ATPases), and rheological properties (capability for aggregation and deformability) 7 days after bloodletting of 12-15 % of the total blood mass. It has been shown that alongside with an elevation of erythrocyte volume and of the number of immature cells - reticulocytes, there was a statistically significant increase of Na,K-ATPase and Ca-ATPase activities in the whole erythrocytes and their membrane preparations - ghosts, the increment of activity in the case of Na,K-ATPase being essentially higher in the whole cells. This indicates the appearance of an enzyme activator inside the erythrocytes. There are also revealed a decrease of firmness of erythrocyte aggregates, a deceleration of spontaneous aggregation, and an increase of index of erythrocyte deformability. The conclusion is made that changes of erythrocyte rheological properties are interconnected with changes of the Na,K-ATPase activity and are directed to optimization of blood circulation in large vessels and capillary network.
Maslova
M N
MN
Kazennov
A M
AM
Katiukhin
L N
LN
Novozhilov
A V
AV
Skverchinskaia
E A
EA
Tavrovskaia
T V
TV
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D004903:Erythrocyte Aggregation / Q000502:physiology*; D004906:Erythrocyte Count; D004910:Erythrocyte Membrane / Q000201:enzymology / Q000502:physiology; D004912:Erythrocytes / Q000166:cytology / Q000201:enzymology / Q000502:physiology; D006470:Hemorrhage / Q000097:blood*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase