Photoinduced crosslinking of double-helical DNA by psoralen covalently linked to a triple helix-forming oligonucleotide under near-physiological conditions.
Stable triplexes have been generated under near-physiological conditions by the introduction of the C and T base analogues 3-methyl-2-aminopyridine-2'-deoxyriboside and 5-(3-aminoprop-2-ynyl)-'-deoxyuridine into psoralen-conjugated triplex-forming oligonucleotides. After irradiation with UV light at 365 nm, photo-induced cross-linking of the TFO to double-helical DNA was observed by UV-melting analysis and fluorescence measurements.
Li
Hong
H
School of Chemistry, University of Southampton, Highfield, Southampton, United Kingdom.
Broughton-Head
Victoria J
VJ
Fox
Keith R
KR
Brown
Tom
T
D001483:Base Sequence; D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects; D015195:Drug Design; D011564:Furocoumarins / Q000737:chemistry* / Q000528:radiation effects; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry* / Q000528:radiation effects; D010777:Photochemistry; D017319:Photosensitizing Agents / Q000737:chemistry / Q000528:radiation effects; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D011564:Furocoumarins; D009841:Oligonucleotides; D017319:Photosensitizing Agents; D004247:DNA