[Effects of short-term continuous shear stress on cells growth and recovery of Laminaria japonica gametophytic cells in photobioreactor].
Laminaria japonica gametophytic cells were cultivated in a photobioreactor under continuous shear stress (0-1000 r/min) in 60 hours and the following static cultivation within 23.5 days. The content of chlorophyll a reached the maximum value of 2.36 mg/L at the end of continuous shear stress when the agitation speed was 90 r/min, while the chlorophyll a (chl a) concentration decreased quickly and nitrogen and phosphorus were released under high shear force (270-1000 r/min). The cell injury ratio at 1000r/min was as 18 times as that of the control. During the recovery course, gametophytic cells showed themselves distinct recovery capability at all agitation speeds. Furthermore, the content of chl a is a more exact index as biomass than dry cells weight (DCW). Besides cell injury ratio, the liberation of phosphorus demonstrates the cells injury.
Huang
Na
N
Center of Cell Culture and Bioprocess Engineering, Shanghai Jiaotong University, Shanghai 200240, China.
Chen
Si-Ye
SY
Qi
Han-Shi
HS
D019149:Bioreactors; D018929:Cell Culture Techniques; D005718:Gametogenesis / Q000502:physiology*; D005854:Germ Cells / Q000502:physiology*; D007795:Laminaria / Q000254:growth & development* / Q000528:radiation effects; D008027:Light; D033081:Shear Strength; D013314:Stress, Mechanical