Quantitative determination of paraquat in meconium by sodium borohydride-nickel chloride chemical reduction and gas chromatography/mass spectrometry (GC/MS).
The objective of this study was to develop a procedure for the GC/MS assay of paraquat in meconium as a biomarker of fetal exposure to paraquat. The method involved a sodium borohydride-nickel chloride reduction procedure, liquid-liquid extraction of the perhydrogenated product, concentration, and GC/MS assay. The method demonstrated good overall recovery (102.56%) with %CV (inter-assay) of less than 13%, and a limit of detection of 0.0156microg/g. Analysis of meconium samples from a study population in the Philippines (n=70) showed a 2.8% prevalence of fetal exposure to paraquat.
Department of Pediatrics, School of Medicine, Wayne State University, 421 East Canfield St., Detroit, MI 48201, USA.
Norberto C
NC
Posecion
Enrique M
EM
Ostrea
Dawn M
DM
Bielawski
D001894:Borohydrides / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006540:Herbicides / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn; D008470:Meconium / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D010269:Paraquat / Q000032:analysis*; D012680:Sensitivity and Specificity
D001894:Borohydrides; D006540:Herbicides; C022838:nickel chloride; D009532:Nickel; C025364:sodium borohydride; D010269:Paraquat