Expression of glucagon-like peptide-1 in the taste buds of rat circumvallate papillae.
Glucagon-like peptide-1 (GLP-1), a 30-amino-acid peptide hormone, is a typical peptide of the brain-gut axis and can affect the metabolism of various tissues and organs. GLP-1 is secreted by intestinal L cells in response to nutrient ingestion. Some studies have shown that taste signaling elements were co-expressed in enteroendocrine cells of the small intestine, and in particular by L-cells. The present study was performed to explore the protein and mRNA expression of GLP-1 in the taste buds of rat circumvallate papillae and to try to determine the significance of its secretion. GLP-1 immunoreactivity was observed in spindle-shaped taste bud cells, with positive cells displaying a characteristic distribution of reaction product that was confined to the cytosol. Reverse transcription polymerase chain reaction (RT-PCR) assay showed that GLP-1 mRNA was expressed in circumvallate papillae. The expression of GLP-1 suggests that it may play an important role in the taste stimulation of nutrients and gut hormone secretion.
Feng
Xiao-Hong
XH
Department of Endocrinology, The First Clinical Hospital of Harbin Medical University, Harbin 150001, China.
Liu
Xiao-Min
XM
Zhou
Li-Hong
LH
Wang
Jing
J
Liu
Guo-Dong
GD
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015870:Gene Expression; D020869:Gene Expression Profiling; D052216:Glucagon-Like Peptide 1 / Q000032:analysis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013650:Taste Buds / Q000378:metabolism*
D000906:Antibodies; D052216:Glucagon-Like Peptide 1