Study of the genetic stability of measles virus CAM-70 vaccine strain after serial passages in chicken embryo fibroblasts primary cultures.
To evaluate the genetic stability of the CAM-70 measles vaccine strain we have performed 10 serial passages of the seed lot virus FMS-7 in chicken embryo fibroblasts primary cultures (CEF) under production conditions. The nucleotide sequences of the seed lot virus, the virus from a vaccine vial (third passage) and from the 10th passage were determined and compared with each other and with sequences from other sources. The full genome analysis of the CAM-70 vaccine still considers it as the most divergent among all vaccine strains. The nucleotide sequence analyses of viral genomes from the three CAM-70 passage levels have demonstrated that they are identical. This study shows that the measles CAM-70 vaccine virus is highly adapted to its cultivation conditions and that its genetic stability contributes, in part, to the safety profile of the vaccine.
Borges
Maria Beatriz
MB
Bio-Manguinhos, Fundação Oswaldo Cruz, Av. Brasil, 4365 - Manguinhos, Rio de Janeiro, RJ 21040-360, Brazil. borges@bio.fiocruz.br
Caride
Elena
E
Jabor
Alfredo V
AV
Malachias
José Marcus N
JM
Freire
Marcos S
MS
Homma
Akira
A
Galler
Ricardo
R
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002642:Chick Embryo; D005347:Fibroblasts; D016679:Genome, Viral; D006801:Humans; D008457:Measles / Q000821:virology; D008458:Measles Vaccine / Q000235:genetics*; D008459:Measles virus / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation; D016415:Sequence Alignment; D012692:Serial Passage
D008458:Measles Vaccine