A Bayesian reconstruction method for micro-rotation imaging in light microscopy.
The authors present a three-dimensional (3D) reconstruction algorithm and reconstruction-based deblurring method for light microscopy using a micro-rotation device. In contrast to conventional 3D optical imaging where the focal plane is shifted along the optical axis, micro-rotation imaging employs dielectric fields to rotate the object inside a fixed optical set-up. To address this entirely new 3D-imaging modality, the authors present a reconstruction algorithm based on Bayesian inversion theory and use the total variation function as a structure prior. The spectral properties of the reconstruction by simulations that illustrate the strengths and the weaknesses of the micro-rotation approach, compared with conventional 3D optical imaging, were studied. The reconstruction from real data sets shows that this method is promising for 3D reconstruction and offers itself as a deblurring method using a reconstruction-based procedure for removing out-of-focus light from the micro-rotation image series.
Laksameethanasan
Danai
D
Laboratory of Computational Engineering, Helsinki University of Technology, FI-02015 TKK, Finland. danai.laksameethanasan@tkk.fi
Brandt
Sami S
SS
Engelhardt
Peter
P
Renaud
Olivier
O
Shorte
Spencer L
SL
D000818:Animals; D001499:Bayes Theorem; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D009685:Nuclear Envelope / Q000648:ultrastructure; D012399:Rotation