Establishment of canine RNA polymerase I-driven reverse genetics for influenza A virus: its application for H5N1 vaccine production.
In the event of a new influenza pandemic, vaccines whose antigenicities match those of circulating strains must be rapidly produced. Here, we established an alternative reverse genetics system for influenza virus using the canine polymerase I (PolI) promoter sequence that works efficiently in the Madin-Darby canine kidney cell line, a cell line approved for human vaccine production. Using this system, we were able to generate H5N1 vaccine seed viruses more efficiently than can be achieved with the current system that uses the human PolI promoter in African green monkey Vero cells, thus improving pandemic vaccine production.
Murakami
Shin
S
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Horimoto
Taisuke
T
Yamada
Shinya
S
Kakugawa
Satoshi
S
Goto
Hideo
H
Kawaoka
Yoshihiro
Y
D000818:Animals; D002460:Cell Line; D004285:Dogs; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics*; D009980:Influenza A virus / Q000235:genetics*; D007252:Influenza Vaccines / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012318:RNA Polymerase I / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis; D014764:Viral Proteins / Q000096:biosynthesis
D007252:Influenza Vaccines; D012367:RNA, Viral; D014764:Viral Proteins; D012318:RNA Polymerase I