cDNA cloning and characterization of temperature-acclimation-associated light meromyosins from grass carp fast skeletal muscle.
The three types of cDNA clones, previously defined as the 10 degrees C, intermediate and 30 degrees C-types [Tao, Y., Kobayashi, M., Liang, C.S., Okamoto, T., Watabe, S., 2004. Temperature-dependent expression patterns of grass carp fast skeletal myosin heavy chain genes. Comp. Biochem. Physiol. B 139, 649-656], were determined for their 5'-regions which encoded at least the C-terminal half of myosin rod, light meromyosin (LMM), in fast skeletal muscles of grass carp Ctenopharyngodon idella. The deduced amino acid sequence identity was 91.1% between the 10 degrees C and 30 degrees C-types and 91.4% between the 10 degrees C and intermediate-types, whereas a high sequence identity of 97.8% was found between the intermediate and 30 degrees C-types. These three grass carp LMMs all had a characteristic seven-residue (heptad) repeat (a, b, c, d, e, f, g)(n), where positions a and d were normally occupied by hydrophobic residues, and positions b, c and f by charged residues. However, the ratios of hydrophobic residues to the total were higher for the intermediate- and 30 degrees C- than 10 degrees C-type LMM, suggesting that the former both types may form more stable coiled-coils of alpha-helices than the latter type. These differences in the primary structures of LMM isoforms might be partially implicated in differences in the thermostabilities and gel-forming profiles of myosins from grass carp in different seasons reported previously [Tao, Y., Kobayashi, M., Fukushima, H., Watabe, S., 2005. Changes in enzymatic and structural properties of grass carp fast skeletal myosin induced by the laboratory-conditioned thermal acclimation and seasonal acclimatization. Fish. Sci. 71, 195-204; Tao, Y., Kobayashi, M., Fukushima, H., Watabe, S., 2007. Changes in rheological properties of grass carp fast skeletal myosin induced by seasonal acclimatization. Fish. Sci. 73, 189-196].
College of Food Science and Technology, Shanghai Fisheries University, Shanghai 200090, China.
Sun-Yong
SY
Wang
Yan
Y
Tao
Chun-Shi
CS
Liang
Hideto
H
Fukushima
Shugo
S
Watabe
D000064:Acclimatization / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D057927:Hydrophobic and Hydrophilic Interactions; D008969:Molecular Sequence Data; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D015879:Myosin Subfragments / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics; D017433:Protein Structure, Secondary; D012621:Seasons; D017386:Sequence Homology, Amino Acid; D013696:Temperature
D018076:DNA, Complementary; D015879:Myosin Subfragments; D020033:Protein Isoforms