[Study on the variation of lymphocytes and cytokines in patients with echinococcosis granulosus].
OBJECTIVE
To investigate the variation of lymphocytes and cytokines in patients with cystic echinococcosis (CE).


METHODS
80 CE patients who were diagnosed for the first time (60 of Han and 20 of Uygur nationality), and 37 patients who were to accept the second surgical operation(24 Han and 13 Uygur nationality) were included in the study. The peripheral lymphocytes of patients before operation were analyzed by flow cytometry (FCM) to detect T and B lymphocytes, NK cells bearing surface markers, as well as Thl cytokine IFN-gamma and Th2 cytokine IL-4 in the cytoplasm of lymphocytes. 179 healthy persons served as control.


RESULTS
In the group of Han patients who were diagnosed for the first time, the percentage of total T cells(CD3+) was lower than the control (P<0.05), while among patients accepting the second operation, the ratio of total T cells showed no difference to the control. For the helper T cells (CD3+/CD4+), NK cells (CD3+/CD16,56+) and B cells (CD3+/CD19+), their ratio were significantly lower in both groups than the control (P<0.01), but their cytotoxic T cells (CD3+/CD8+) were higher than the control. In Uygur patients diagnosed for the first time, B cell ratio was lower than that in control (P<0.05). The NK cell level in both groups of patients was lower than control (P<0.01 and P<0.05 respectively). The level of ThO and Th1 showed no statistical difference among the three groups (P>0.05). The Th2 level was significantly higher in the first diagnosed patients than control (P<0.01), but no statistical difference between the group with the second operation and the other two groups.


CONCLUSIONS
The immune status of echinococcosis patients is inhibited and the level of Th1 and Th2 shows difference in the first and second operation groups.
Key Laboratory of Xinjiang Hydatid Institute, Urumqi 830054, China.
Yan
Y
Zhang
Hao
H
Wen
Ren-yong
RY
Lin
Xiao-mei
XM
Lu
Xue
X
Zhang
Xiao-hui
XH
Feng
Jin-ming
JM
Zhao
Jing-ping
JP
Zhang
D000818:Animals; D018941:Antigens, CD19 / Q000097:blood; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism; D017252:CD3 Complex / Q000097:blood; D015704:CD4 Antigens / Q000097:blood; D016827:CD8 Antigens / Q000097:blood; D002681:China; D016207:Cytokines / Q000378:metabolism*; D004443:Echinococcosis / Q000097:blood / Q000378:metabolism / Q000469:parasitology*; D048209:Echinococcus granulosus / Q000502:physiology*; D005434:Flow Cytometry; D006790:Host-Parasite Interactions; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D007694:Killer Cells, Natural / Q000166:cytology / Q000378:metabolism; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism
D018941:Antigens, CD19; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D016207:Cytokines; D015847:Interleukin-4; D007371:Interferon-gamma