Lysophosphatidic acid-induced interleukin-1 beta expression is mediated through Gi/Rho and the generation of reactive oxygen species in macrophages.
Lysophosphatidic acid (LPA), a low-molecular-weight lysophospholipid enriched in platelets and mildly oxidized low-density lipoproteins, is known to regulate inflammation and atherosclerosis by binding to its cognate receptors. In this study, we reported that LPA upregulated interleukin-1 beta (IL-1 beta) expression in mouse J774A.1 macrophages. By using pharmacological inhibitors, it was suggested that G(i)/Rho activation and subsequent reactive oxygen species (ROS) production were involved in IL-1 beta induction. In addition, IL-1 beta induction by LPA was also observed in human primary macrophages. In summary, LPA is involved in the processes of inflammation by affecting macrophage behavior.
Chang
Chi-Lun
CL
Institute of Zoology, National Taiwan University, Taipei, Taiwan.
Lin
Mu-En
ME
Hsu
Hsien-Yeh
HY
Yao
Chao-Ling
CL
Hwang
Shiaw-Min
SM
Pan
Chien-Yuan
CY
Hsu
Chi-Yen
CY
Lee
Hsinyu
H
D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000502:physiology*; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism*; D008246:Lysophospholipids / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D017931:DNA Primers; D053583:Interleukin-1beta; D008246:Lysophospholipids; D017382:Reactive Oxygen Species; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; C032881:lysophosphatidic acid