Characterization of dehydromonacolin-MV2 from Monascus purpureus mutant.
OBJECTIVE
Characterization of dehydromonacolin-MV2, a bioactive metabolite isolated from Monascus purpureus mutant (CFR 410-11).


RESULTS
Chloroform extract of rice, fermented with a hyperpigment-producing mutant of M. purpureus (CFR 410-11) was found to contain metabolites that inhibited the growth of Bacillus, Pseudomonas and Streptococcus in agar gel diffusion assays. The extract inhibited lipid peroxidation and scavenged 2,2-diphenyl-1-pycrylhydrazyl and hydroxyl radicals. The active compound purified by silica gel column chromatography was characterized by NMR. The carbon, proton and 2D HSQCT assignments identified dehydromonacolin-MV2 as the bioactive metabolite.


CONCLUSIONS
Dehydromonacolin-MV2 apparently originated in the mutant by hydroxylation and oxidation of monacolin-J, an intermediate of monacolin biosynthetic pathway.


CONCLUSIONS
Identification of the production of dehydromonacolin-MV2 by M. purpureus mutant (CFR 410-11) is new to literature. Bioactive properties of the compound suggested its pharmaceutical applications.
Dhale
M A
MA
Department of Food Microbiology, Central Food Technological Research Institute, Mysore-570 020, India.
Divakar
S
S
Umesh-Kumar
S
S
Vijayalakshmi
G
G
D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification*; D013058:Mass Spectrometry; D032741:Monascus / Q000737:chemistry* / Q000235:genetics; D009154:Mutation; D009172:Mycology; D009281:Naphthalenes / Q000032:analysis* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
D000900:Anti-Bacterial Agents; D009281:Naphthalenes; C527761:dehydromonacolin-MV2; C045376:monacolin J