Nutraceutical strategy in aging: targeting heat shock protein and inflammatory profile through understanding interleukin-6 polymorphism.
The aging process is paralleled by two- to fourfold increases in plasma/serum levels of inflammatory mediators, such as cytokines and acute-phase proteins. In this study we assessed the inflammatory profile and polymorphism of healthy elderly subjects and the influence of a nutraceutical supplement. Forty elderly, generally healthy subjects were recruited, divided into two matched groups, and given either a fermented papaya preparation 9 g/day by mouth or the same amount of placebo. Treatments were carried out in a cross-over manner with a 3-month supplementation period followed by a 6-week washout period between treatments. Ten healthy young subjects served as controls. Interleukin-6 (IL-6) promoter -174 G/C polymorphism genotype was determined together with blood levels for redox status, proinflammatory cytokines, high sensitivity C-reactive protein, and serum 70 kDa heat shock protein (Hsp70) concentrations. Tumor necrosis factor-alpha and IL-6 were higher in elderly subjects (P < 0.05 versus young controls). The concentration of Hsp70 inversely correlated with markers of inflammation in -174 G/C-negative subjects (r = 0.62, P < 0.05). Nutraceutical intervention normalized the inflammatory parameters (P < 0.05) with a rise of Hsp70 (P < 0.05). This suggests that healthy elderly individuals may have a proinflammatory profile playing as a downregulating factor for inducible Hsp70, particularly if -174 G/C-negative. A nutraceutical intervention seems able to beneficially modulate such a phenomenon.
Marotta
F
F
G.A.I.A Age-Management Foundation, Pavia, Italy. fmarchimede@libero.it
Koike
K
K
Lorenzetti
A
A
Naito
Y
Y
Fayet
F
F
Shimizu
H
H
Marandola
P
P
D000284:Administration, Oral; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood* / Q000187:drug effects / Q000235:genetics*; D002097:C-Reactive Protein / Q000032:analysis / Q000235:genetics; D029441:Carica / Q000737:chemistry; D018592:Cross-Over Studies; D018840:HSP70 Heat-Shock Proteins / Q000097:blood / Q000235:genetics; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood; D015850:Interleukin-6 / Q000097:blood* / Q000235:genetics*; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D028321:Plant Preparations / Q000008:administration & dosage* / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011897:Random Allocation; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000235:genetics
D018840:HSP70 Heat-Shock Proteins; C508600:IL6 protein, human; D018836:Inflammation Mediators; D015850:Interleukin-6; D028321:Plant Preparations; D014409:Tumor Necrosis Factor-alpha; D002097:C-Reactive Protein