Cytostatic drugs differentially affect phenotypic features of porcine coronary artery smooth muscle cell populations.
We studied the effects of cytostatic drugs on porcine coronary artery spindle-shaped (S) and rhomboid (R) smooth muscle cell (SMC) biological activities related to intimal thickening (IT) formation. Imatinib, and to a lesser extent curcumin, decreased proliferation of S- and R-SMCs and migratory and urokinase activities of R-SMCs more efficiently compared with cyclosporine plus rapamycin. Imatinib increased the expression of alpha-smooth muscle actin in both SMC populations and that of smoothelin in S-SMCs. It decreased S100A4 expression in R-SMCs. By promoting SMC quiescence and differentiation imatinib and curcumin may represent valid candidates for restenosis preventive and therapeutic strategies.
Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, Rue Michel Servet 1, 1211 Geneva 4, Switzerland.
Marco
M
Prunotto
Marc
M
Bacchetta
Swaminathan
S
Jayaraman
Marco
M
Galloni
Guillaume
G
Van Eys
Giulio
G
Gabbiani
Marie-Luce
ML
Bochaton-Piallat
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003331:Coronary Vessels / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D054697:Cytostatic Agents / Q000494:pharmacology*; D015151:Immunoblotting; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D010641:Phenotype; D009418:S100 Proteins / Q000378:metabolism; D013552:Swine; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism
D015415:Biomarkers; D054697:Cytostatic Agents; D009418:S100 Proteins; D014568:Urokinase-Type Plasminogen Activator