Substrate specificity of Stenotrophomonas nitritireducens in the hydroxylation of unsaturated fatty acid.
An isolated bacterium that converted unsaturated fatty acids to hydroxy fatty acids was identified as Stenotrophomonas nitritireducens by API analysis, cellular fatty acids compositions, sequencing the full 16S ribosomal ribonucleic acid, and evaluating its nitrite reduction ability. S. nitritireducens has unique regio-specificity for C16 and C18 cis-9 unsaturated fatty acids. These fatty acids are converted to their 10-hydroxy fatty acids without detectable byproducts. Among the cis-9-unsaturated fatty acids, S. nitritireducens showed the highest specificity for linoleic acid. The cells converted 20 mM linoleic acid to 13.5 mM 10-hydroxy-12(Z)-octadecenoic acid at 30 degrees C and pH 7.5 with a yield of 67.5% (mol/mol).
Yu
In-Sik
IS
Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701, South Korea.
Yeom
Soo-Jin
SJ
Kim
Hye-Jung
HJ
Lee
Jung-Kul
JK
Kim
Yong-Hwi
YH
Oh
Deok-Kun
DK
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006900:Hydroxylation; D007723:Korea; D009573:Nitrites / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology; D020588:Stenotrophomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005231:Fatty Acids, Unsaturated; D009573:Nitrites; D012336:RNA, Ribosomal, 16S; D012722:Sewage