Production of streptomycin from chitin using Streptomyces griseus in bioreactors of different configuration.
Streptomyces griseus was cultured in three different bioreactors in a medium containing chitin flakes. When a conventional bioreactor stirred by two sets of Rushton impellers and operated at high speed was used, the yield of streptomycin (3.1mg/l) was the highest observed and occurred at approximately 500 h. Cultivation of S. griseus in a bioreactor stirred at low speed by a U-shaped paddle resulted in a lower yield of streptomycin (1.8 mg/l) but this was achieved in a shorter period of time (400 h). Increasing the concentration of chitin from 5% to 10% w/v had no significant effect on either of these two parameters. The use of a novel vertical basket bioreactor in which the chitin flakes were contained within a wire mesh basket and were gently fluidised by air, enabled comparatively high yields of streptomycin (2.8 mg/l) in the relatively short time of 300 h.
Department of Chemical Engineering, Loughborough University, Loughborough, Leics LE11 3TU, UK.
Richard J L
RJ
Meanwell
Gilbert
G
Shama
D018533:Biomass; D019149:Bioreactors; D002245:Carbon Dioxide / Q000032:analysis; D002686:Chitin / Q000378:metabolism*; D003470:Culture Media; D004867:Equipment Design; D005612:Freeze Drying; D013305:Streptomyces griseus / Q000254:growth & development / Q000378:metabolism*; D013307:Streptomycin / Q000096:biosynthesis*
D003470:Culture Media; D002686:Chitin; D002245:Carbon Dioxide; D013307:Streptomycin