Comment on "Wide-field coherent anti-Stokes Raman scattering microscopy with non-phase-matching illumination".
We comment on a Letter by Toytman et al. [Opt. Lett.32, 1941 (2007)] in which a novel setup for wide-field imaging in coherent anti-Stokes Raman scattering (CARS) microscopy is demonstrated. There the authors state that our phase-matching implementation of a wide-field CARS system [Appl. Phys. Lett.84, 816 (2004); New J. Phys.8, 36 (2006)] suffers from a strong background from the bulk medium. However, our results show quite the contrary, i.e., that our setup provides a very good signal contrast, due to an almost vanishing background level from the bulk solvent.
Division for Biomedical Physics, Innsbruck Medical University, Austria.
Christoph
C
Heinrich
Stefan
S
Bernet
Monika
M
Ritsch-Marte