Effect of human bone marrow stromal cells and dermal fibroblasts on collagen synthesis and epithelization.
In a previous in vitro study, the authors reported that bone marrow stromal cells (BSCs) have better wound-healing activities than fibroblasts. The purpose of this study was to evaluate the effect of BSCs and fibroblasts on wound-healing activity in vivo. Cultured human BSCs and dermal fibroblasts taken from the same patients were tested to compare collagen synthesis and epithelization in a rat wound model. No-cell-treated animals were used as controls. The BSC group showed the highest collagen level, followed by the fibroblast group, and then the no-cell group (P < 0.05). In addition, the best epithelization was observed in the BSC group. These results demonstrate that BSCs better stimulate wound healing than fibroblasts in vivo and in vitro.
Lee
Chi-Ho
CH
Department of Plastic Surgery, Kangwon National University College of Medicine, Choonchun Korea.
Han
Seung-Kyu
SK
Choi
Won-Il
WI
Kim
Woo-Kyung
WK
D000328:Adult; D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D003094:Collagen / Q000096:biosynthesis*; D020405:Dermis / Q000166:cytology* / Q000378:metabolism*; D004848:Epithelium / Q000254:growth & development*; D005347:Fibroblasts / Q000378:metabolism* / Q000637:transplantation*; D006801:Humans; D008297:Male; D008875:Middle Aged; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000637:transplantation*
D003094:Collagen