Rapid detection of ESBL-producing Klebsiella pneumoniae in blood cultures by fluorescent in-situ hybridization.
Multi-resistant Enterobacteriaceae pose a serious threat of hospital acquired infections and their rapid identification is important for better clinical outcome. This study describes the rapid identification of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae of the sulphydryl variable-type by fluorescent in-situ hybridization. The method which rapidly identifies the target genes within 1 h could be a potentially rapid bacterial diagnostic tool.
Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia.
Selvi
S
Palasubramaniam
Sekaran
S
Muniandy
Parasakthi
P
Navaratnam
D001769:Blood / Q000382:microbiology*; D003470:Culture Media; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007710:Klebsiella Infections / Q000175:diagnosis / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D015345:Oligonucleotide Probes; D018410:Pneumonia, Bacterial / Q000175:diagnosis / Q000382:microbiology; D012680:Sensitivity and Specificity; D013997:Time Factors; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
D003470:Culture Media; D015345:Oligonucleotide Probes; C117193:beta-lactamase SHV-5; D001618:beta-Lactamases