[Identification and expression of non-coding RNAs NC28 and NC119 in human tumors].
OBJECTIVE
To explore and identify the non-coding RNAs related to tumors.


METHODS
We used RT-PCR and Northern blot to analyze non-coding RNAs in tumor tissues and cell lines.


RESULTS
Two predicted non-coding RNAs were confirmed to be expressed in cancer tissues and cell lines by RT-PCR and DNA sequencing. We detected the expression of two non-coding RNA transcripts by Northern blot. The length of NC28 was about 1800 nt, and that of NC119 was about 1200nt.


CONCLUSIONS
NC28 and NC119 have a tumor-associated expression pattern. The non-coding RNAs may play a role in the development of tumors.
Sun
Zhen-yuan
ZY
Department of Liver Surgery, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Yuan
Yan-hua
YH
Liu
Chang-ning
CN
Zhao
Yi
Y
Huang
Jie-fu
JF
Sang
Xin-ting
XT
Mao
Yi-lei
YL
Lu
Xin
X
Wang
Yu
Y
Chang
Yong-sheng
YS
Zhao
Hai-tao
HT
D045744:Cell Line, Tumor; D006801:Humans; D009369:Neoplasms / Q000378:metabolism*; D022661:RNA, Untranslated / Q000096:biosynthesis*
D022661:RNA, Untranslated