Modeling morphogenesis and oncogenesis in three-dimensional breast epithelial cultures.
Three-dimensional (3D) epithelial culture systems recreate the cardinal features of glandular epithelium in vivo and represent a valuable tool for modeling breast cancer initiation and progression in a structurally appropriate context. 3D models have emerged as a powerful method to interrogate the biological activities of cancer genes and oncogenic pathways, and recent studies have poignantly illustrated their utility in dissecting the emerging role of tensional force in regulating epithelial tissue homeostasis. We review how 3D models are being used to investigate fundamental cellular and biophysical mechanisms associated with breast cancer progression that have not been readily amenable to traditional genetic or biochemical analysis.
Department of Surgery and Center for Bioengineering and Tissue Regeneration, University of California, San Francisco, CA, USA. Christy.Hebner@gilead.com
Christy
C
Hebner
Valerie M
VM
Weaver
Jayanta
J
Debnath
D001940:Breast / Q000473:pathology*; D001943:Breast Neoplasms / Q000209:etiology / Q000473:pathology*; D018929:Cell Culture Techniques; D002471:Cell Transformation, Neoplastic; D004847:Epithelial Cells / Q000473:pathology / Q000502:physiology; D005260:Female; D006801:Humans; D008954:Models, Biological; D009024:Morphogenesis; D009857:Oncogenes / Q000502:physiology*