Triplex formation using oligonucleotide clamps carrying 8-aminopurines.
The synthesis and properties of triplex-forming DNA clamps carrying 8-aminopurines are described. The stability of triple helices is enhanced by replacing purine bases with 8-aminopurine residues. These enhanced binding properties are used for the specific capture of polypyrimidine RNA/DNA sequences of interest.
Aviñó
Anna
A
Instituto de Biologia Molecular de Barcelona, C.S.I.C., Barcelona, Spain.
Grimau
Marta G
MG
Alvira
Margarita
M
Eritja
Ramon
R
Gargallo
Raimundo
R
Orozco
Modesto
M
González
Carlos
C
D017796:Caulimovirus / Q000235:genetics; D004247:DNA / Q000737:chemistry; D004279:DNA, Viral / Q000235:genetics; D015195:Drug Design; D004355:Drug Stability; D006860:Hydrogen Bonding; D008087:Listeria / Q000235:genetics; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D011401:Promoter Regions, Genetic; D011685:Purine Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D012329:RNA, Bacterial / Q000235:genetics
D004279:DNA, Viral; D015335:Molecular Probes; D009841:Oligonucleotides; D011685:Purine Nucleotides; D012329:RNA, Bacterial; D004247:DNA