Molecular biomarkers of Dreissena polymorpha for evaluation of renaturation success of a formerly sewage polluted stream.
The renaturation success of an urban stream, formally used for discharge of treated sewage waters was investigated by active biomonitoring with Dreissena polymorpha based on molecular biomarkers and compared to a semi-natural stream and laboratory controls. Response to pollution charges were analyzed by reverse transcriptase-PCR of heat-shock protein (hsp70), P-glycoprotein (P-gp), catalase (CAT) and pi class glutathione S-transferase (piGST). Hsp70 transcription was similarly induced at both sites, indicating protein damage. At the semi-natural stream CAT and P-gp were induced, indicating oxidative stress and increased discharge of pollutants, which correlated to high amounts of aluminum at this site. piGST was induced at one sampling date at the renaturated stream only, but identification of the causing pollutant was not achieved. Results confirm regeneration of the formerly sewage polluted stream, because induction of the tested biomarkers was either at or below the levels of the semi-natural stream.
Contardo-Jara
Valeska
V
Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Department of Inland Fisheries, Biochemical Regulation, Müggelseedamm 301, 12587 Berlin, Germany. contardo@igb-berlin.de
Wiegand
Claudia
C
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D000818:Animals; D001673:Biodegradation, Environmental; D015415:Biomarkers; D002374:Catalase / Q000235:genetics; D049875:Dreissena / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods*; D005858:Germany; D005982:Glutathione Transferase / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D018384:Oxidative Stress; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045483:Rivers; D012722:Sewage; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D012722:Sewage; D014874:Water Pollutants, Chemical; D002374:Catalase; D005982:Glutathione Transferase