Osteoarthritis models after anterior cruciate ligament resection and medial meniscectomy in rats. A histological and immunohistochemical study.
OBJECTIVE
To compare the amount of degeneration based on the time spent, using 2 different methods of surgically induced osteoarthritis (OA) that frequently used in treating OA.


METHODS
We undertook this research in Ondokuz Mayis University, Surgical Research Center between April 2006 and July 2006. In this study, 55 rats were used, 7 as the control group, and 12 in each of 4 groups. We compared the amount of OA formed by the medial meniscectomy (MMx) and anterior cruciate ligament transection (ACLT) at 8 and 16 weeks according to the Modified Mankin Score and histologically and immunohistochemically due to their response to Matrix metalloproteinase 13 expression (MMP13).


RESULTS
We observed the highest degeneration in the MMx model at 8 weeks, and this situation continued until 16 weeks. However, the degeneration in the ACLT model was lower at 8 weeks compared with the MMx group, however, it reached the same amount as the MMX group at 16 weeks.


CONCLUSIONS
The OA model formed by the ACLT method was better than the MMx model when degeneration and time were taken into consideration and should be used when researching drugs on an experimental basis in OA.
Department of Orthopaedics, Faculty of Medicine, University of Ondokuz Mayis, Samsun, Turkey.
Ahmet
A
Piskin
Mustafa Y
MY
Gulbahar
Yilmaz
Y
Tomak
Birol
B
Gulman
Murat
M
Hokelek
Servet
S
Kerimoglu
Bulent
B
Koksal
Taner
T
Alic
Yonca B
YB
Kabak
D000818:Animals; D016118:Anterior Cruciate Ligament / Q000601:surgery*; D004195:Disease Models, Animal; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D008592:Menisci, Tibial / Q000601:surgery*; D010003:Osteoarthritis / Q000201:enzymology / Q000209:etiology* / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D053509:Matrix Metalloproteinase 13