Phosphatidylcholine is required for the efficient formation of photosynthetic membrane and B800-850 light-harvesting complex in Rhodobacter sphaeroides.
No phosphatidylcholine (PC) was detected in the membrane of Rhodobacter sphaeroides pmtA mutant (PmtA1) lacking phosphatidylethanolamine (PE) N-methyltransferase, whereas PE in the mutant was increased up to the mole % comparable to the combined level of PE and PC of wild type. Neither the fatty acid composition nor the fluidity of membrane was altered by pmtA mutation. Consistently, aerobic and photoheterotrophic growth of PmtA1 were not different from wild type. However, PmtA1 showed an extended lag phase (15 h) after the growth transition from aerobic to photoheterotrophic conditions, indicating the PC requirement for the efficient formation of intracytoplasmic membrane (ICM). Interestingly, the B800-850 complex of PmtA1 was decreased more than twofold in comparison with wild type, whereas the level of the B875 complex comprising the fixed photosynthetic unit was not changed. Since puc expression is not affected by pmtA mutation, PC appears to be required for the proper formation of the B800-850 complex in the ICM of R. sphaeroides.
Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul 121-742, Korea.
Eui-Jin
EJ
Kim
Mi-Sun
MS
Kim
Jeong K
JK
Lee
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000502:physiology; D045342:Light-Harvesting Protein Complexes / Q000096:biosynthesis*; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000032:analysis; D008780:Methyltransferases / Q000502:physiology; D010713:Phosphatidylcholines / Q000502:physiology*; D010788:Photosynthesis; D012242:Rhodobacter sphaeroides / Q000254:growth & development / Q000378:metabolism*
C081521:B800-850 light-harvesting complex, bacteria; D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D008780:Methyltransferases; C435200:PmtA protein, bacteria