[Immunoquantification of serum lipoprotein(a) in healthy Ivorian subject: a comparative evaluation of two methods].
We have determined the concentration of Lp(a) in an Ivory Coast population (n=102) using two immunochemical methods: Laurell's monodimensional electro-immunodiffusion (EID) and immunonephelometry (IN). Within-run and between-run precision was respectively 3.07% and 3.97% by IN and 1.52% and 4.48% by EID method. As regard the exactitude, the bias goals in two methods were 3.5% and 3.0% respectively with IN and EID. The two methods were correlated (r=0.84; p=0.006). Mean values of Lp(a) were significantly (p=0.0007) higher by IN than EID: 0.48+/-0.34 g/L versus 0.32+/-0.19 g/L. The Lp(a) distributions were non-Gaussian, skewed towards high values, with median value of 0.47 g/L and 0.32 g/L respectively for IN and EID methods. Therefore, we conclude that although both methods showed a satisfactory precision, and results were correlated, Lp(a) values were higher by INP. Furthermore, mean values of Lp(a) in presumed healthy Ivorian is higher than in Caucasians.
Laboratoire de biochimie, UFR de sciences pharmaceutiques et biologiques, Abidjan, Ivory Coast.
A
A
Edjeme-Ake
R
R
Garnotel
E
E
Anglés-Cano
D
D
Monnet
P
P
Gillery
D000293:Adolescent; D000328:Adult; D007560:Cote d'Ivoire; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D005779:Immunodiffusion; D017270:Lipoprotein(a) / Q000097:blood*; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D017270:Lipoprotein(a)