Foxb1-driven Cre expression in somites and the neuroepithelium of diencephalon, brainstem, and spinal cord.
We have knocked-in Cre-IRES-EGFP in the Foxb1 locus by homologous recombination in embryonic stem cells. We removed the PGK-neo cassette (which was flanked by FRT sequences) by crossing with the FLPeR deleter mouse. The Foxb1(Cre) line showed Cre recombinase activity as well as EGFP fluorescence reproducing Foxb1 expression accurately. By crossing Foxb1(Cre) mice with the ROSA26R and Z/AP mouse reporter lines we have been able to trace the lineage of Foxb1-expressing cells. Early transient expression of Foxb1 in the paraxial mesoderm translates into labeling of the somites. In the central nervous system (CNS), the Foxb1 lineage includes the thalamus and mammillary body (hypothalamus), brainstem, and the ventral spinal cord and floor plate.
Department of Genes and Behavior, Max Planck Institute of Biophysical Chemistry, Brain Development Group, D-37077 Göttingen, Germany.
Tianyu
T
Zhao
Xunlei
X
Zhou
Nora
N
Szabó
Michael
M
Leitges
Gonzalo
G
Alvarez-Bolado
D000818:Animals; D001933:Brain Stem / Q000196:embryology / Q000378:metabolism; D002490:Central Nervous System / Q000196:embryology* / Q000378:metabolism; D004027:Diencephalon / Q000196:embryology / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000378:metabolism; D051858:Forkhead Transcription Factors / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D019426:Integrases / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D016254:Mutagenesis, Insertional / Q000379:methods*; D009474:Neurons / Q000378:metabolism; D011995:Recombination, Genetic; D019170:Somites / Q000196:embryology / Q000378:metabolism; D013116:Spinal Cord / Q000196:embryology / Q000378:metabolism
D051858:Forkhead Transcription Factors; C491332:Foxb1 protein, mouse; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases