Sprouty gene expression is regulated by nerve and FGF6 during regeneration of mouse muscles.
Sprouty (Spry) proteins were identified as negative regulators of fibroblast growth factor (FGF) signaling in vertebrates and invertebrates. Given the importance of the FGFs in myogenesis, we performed cardiotoxin injury-induced regeneration experiments on soleus muscles of both, adult control and FGF6 ( - / - ) mutant mice and analyzed the accumulation of Spry (1, 2 and 4) transcripts using semi-quantitative and real-time RT-PCR assays and in situ hybridization. We also analyzed the effects of muscle denervation on the accumulation of Spry transcripts. The three Spry genes begin to be expressed as early as the first stages of muscle regeneration and are characterized by distinct expression patterns. Moreover, Spry gene expression was highly and differentially up-regulated, precociously by the lack of FGF6, and belatedly by muscle denervation strongly suggesting that the transient rise of Spry mRNA accumulation was associated to muscle differentiation. Rescue experiments supported the idea of a specific relationship between FGF6 and Spry 2, both being known for their particular involvement in myogenesis.
Laziz
Iman
I
Equipe Biologie du Développement et de la Différenciation Neuromusculaire, Centre Universitaire des Saints-Pères, Université René Descartes, Paris, France.
Armand
Anne-Sophie
AS
Pariset
Claude
C
Lecolle
Sylvie
S
Della Gaspera
Bruno
B
Charbonnier
Frédéric
F
Chanoine
Christophe
C
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D051522:Fibroblast Growth Factor 6 / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009121:Muscle Denervation; D018482:Muscle, Skeletal / Q000294:innervation / Q000502:physiology*; D020033:Protein Isoforms / Q000096:biosynthesis; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012038:Regeneration
D048868:Adaptor Proteins, Signal Transducing; C486115:Fgf6 protein, mouse; D051522:Fibroblast Growth Factor 6; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D020033:Protein Isoforms; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D017346:Protein Serine-Threonine Kinases; C118389:Spry2 protein, mouse