Single nucleotide polymorphisms of the aromatase gene (CYP19A1), HER2/neu status, and prognosis in breast cancer patients.
OBJECTIVE
Estrogen exposure is involved in both breast cancer susceptibility and the prognosis in patients with breast cancer. Aromatase is involved in the production of estrogens, and altered expression of it might be associated with the prognosis. The aim of this study was to examine the effect of single nucleotide polymorphisms (SNPs) in the aromatase gene, CYP19A1, on the prognosis, and in relation to tumor and patient characteristics in a cohort of breast cancer patients.


METHODS
The cohort analyzed in this study consisted of 1,257 patients with invasive primary breast cancer. Polymorphisms rs10046, rs4646 and rs700519 were genotyped within this group.


RESULTS
The variant genotypes of rs10046 and rs4646 were associated with a lower percentage of HER2-positive tumors. There was no association of rs700519 and rs4646 with disease-free survival (DFS) or overall survival (OS). The variant genotype of rs10046 was significantly associated with a better 5-year DFS (hazards ratio 0.51; 95% CI, 0.32 to 0.81; P=0.004) adjusted for age, nodal status, tumor size grading, and hormone receptor status. This effect appeared to be determined in the subgroup of premenopausal patients.


CONCLUSIONS
SNPs rs10046 and rs4646 may influence the HER2 status of breast cancer tumors, and rs10046 genotypes are associated with an altered DFS. Genotypes of aromatase polymorphisms may influence the prognosis for breast cancer patients not only by affecting the extent of estrogen exposure but also through an alteration in tumor characteristics.
Fasching
Peter Andreas
PA
University Breast Center for Franconia, Department of Gynecology and Obstetrics, Erlangen University Hospital, Erlangen, Germany. peter.fasching@gyn.med.uni-erlangen.de
Loehberg
Christian R
CR
Strissel
Pamela L
PL
Lux
Michael P
MP
Bani
Mayada R
MR
Schrauder
Michael
M
Geiler
Sonja
S
Ringleff
Kerstin
K
Oeser
Sonja
S
Weihbrecht
Sebastian
S
Schulz-Wendtland
Rüdiger
R
Hartmann
Arndt
A
Beckmann
Matthias W
MW
Strick
Reiner
R
D020413:3' Untranslated Regions / Q000235:genetics; D001141:Aromatase / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000556:secondary; D018275:Carcinoma, Lobular / Q000235:genetics / Q000556:secondary; D015331:Cohort Studies; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate
D020413:3' Untranslated Regions; D001141:Aromatase; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases