Differentially expressed genes under cold acclimation in Physcomitrella patens.
Cold acclimation improves freezing tolerance in plants. In higher plants, many advances have been made toward identifying the signaling and regulatory pathways that direct the low-temperature stress response; however, similar insights have not yet been gained for simple nonvascular plants, such as bryophytes. To elucidate the pathways that regulate cold acclimation in bryophytes, we used two PCR-based differential screening techniques, cDNA amplified fragment length polymorphism (cDNA-AFLP) and suppression subtractive hybridization (SSH), to isolate 510 ESTs that are differentially expressed during cold acclimation in Physcomitrella patens. We used realtime RT-PCR to further analyze expression of 29 of these transcripts during cold acclimation. Our results show that cold acclimation in the bryophyte Physcomitrella patens is not only largely similar to higher plants but also displays distinct differences, suggests significant alteration during the evolution of land plants.
College of Life Science, Capital Normal University, Beijing 100037, China.
Ming-Ming
MM
Sun
Lin-Hui
LH
Li
Hua
H
Xie
Rong-Cai
RC
Ma
Yi-Kun
YK
He
D000064:Acclimatization / Q000235:genetics* / Q000502:physiology*; D054458:Amplified Fragment Length Polymorphism Analysis; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D019068:Bryopsida / Q000235:genetics* / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology; D003081:Cold Climate; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005615:Freezing; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013256:Steroids / Q000096:biosynthesis
D017931:DNA Primers; D018744:DNA, Plant; D013256:Steroids