Osteoblast-like cell proliferation on tape-cast and sintered tricalcium phosphate sheets.
The influence of sintering temperature on the in vitro proliferation of osteoblast-like cells to sintered tricalcium phosphate (TCP) sheets prepared by tape-casting was investigated. Green sheets of tape-cast beta-TCP were sintered for 2h in a furnace at atmospheric pressure at five different sintering temperatures: 900, 1000, 1100, 1150 and 1200 degrees C. The number of osteoblast-like (MC3T3-E1) cells deposited onto TCP sheets was counted after cell cultivation for 1week and was found to have increased with increasing sintering temperature. The TCP sheets sintered at 900 degrees C exhibited a significantly lower cell number than TCP sheets sintered at 1000, 1100, 1150 and 1200 degrees C. In the attenuated total reflection infrared spectra, the peaks around 900-1150cm(-1), corresponding to the P-O vibration mode of the phosphate group, gradually decreased and shifted to lower wavenumbers with increasing sintering temperature. Meanwhile, the zeta potential of TCP sintered at 900 degrees C showed a highly negative charge when compared with the other groups. This would suggest that the higher solubility of the TCP sheets sintered at 900 degrees C exerted the higher negative charge obtained from zeta potential measurement. Within the limitations of this study, it was indicated that osteoblast-like cell proliferation increased with increasing sintering temperature. The biological stability of the sintered TCP sheet surface was considered to have affected cell proliferation.
Tanimoto
Yasuhiro
Y
Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho Nishi, Matsudo, Chiba 271-8587, Japan. tanimoto.yasuhiro@nihon-u.ac.jp
Shibata
Yo
Y
Kataoka
Yu
Y
Miyazaki
Takashi
T
Nishiyama
Norihiro
N
D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry; D049109:Cell Proliferation; D006358:Hot Temperature; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D013055:Spectrophotometry, Infrared; D014961:X-Ray Diffraction
D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate