[Comparative proteome analysis of human papillomavirus-infected cervical specimens and the difference between the high- and low-risk genotypes of human papillomavirus].
OBJECTIVE
To perform an comparative proteome analysis of human papillomavirus-infected cervical specimens and to investigate different expressions between high- and low-risk genotypes.


METHODS
The cervical specimens were divided into two groups (cervical intraepithelial neoplasia group and condyloma acuminatum group) according to their genotypes. Using comparative proteome technology, high-risk human papillomavirus-infected cervical intraepithelial neoplasia, low-risk human papillomavirus-infected condyloma acuminatum, and normal cervical intraepithelial tissue were compared. The differential expression protein spots were identified by mass spectrometry.


RESULTS
Totally 26 differential spots were selected and analyzed, and 22 peptide mass fingerprints (PMF) maps were obtained by MALDI-TOF-MS. Eighteen proteins were preliminarily identified after searching the NCBInr database. The function information of these 18 proteins mainly involved cell metabolism, signal transduction, cell secretion, cell cytoskeleton construction, cell proliferation, and apoptosis.


CONCLUSIONS
The proteomic expressions after the cervical infection of high- or low-risk genotype of human papillomavirus are obviously different.
Qi
Shu-zhen
SZ
Department of Venereology, Institute of Dermatology, CAMS and PUMC, Nanjing 210042, China.
Zhang
Guo-cheng
GC
Zhang
Jin-ping
JP
Zeng
Xue-si
XS
Cao
Yuan-hua
YH
Zhong
Ming-ying
MY
Tao
Xiao-hua
XH
Liu
Tong-yun
TY
Wang
Qian-qiu
QQ
Yang
Rong-ge
RG
D002584:Cervix Uteri / Q000378:metabolism; D003218:Condylomata Acuminata / Q000378:metabolism* / Q000821:virology; D005260:Female; D005838:Genotype; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics* / Q000472:pathogenicity; D030361:Papillomavirus Infections / Q000378:metabolism* / Q000821:virology; D020543:Proteome / Q000378:metabolism*; D012306:Risk; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D002577:Uterine Cervical Diseases / Q000378:metabolism* / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000378:metabolism* / Q000821:virology
D020543:Proteome