Isolation and structure determination of streptochlorin, an antiproliferative agent from a marine-derived Streptomyces sp. 04DH110.
An antiproliferative agent, streptochlorin, was isolated from the fermentation broth of a marine actinomycete isolated from marine sediment. Phylogenetic analysis of the 16S rRNA gene sequence indicated that the strain belongs to the genus Streptomyces. Bioactivity guided fractionation of the culture extract by solvent partitioning, ODS open flash chromatography, and reversed-phase HPLC gave a pure compound, streptochlorin. Its structure was elucidated by extensive 2D NMR and mass spectral analyses. Streptochlorin exhibited significant antiproliferative activity against human cultured cell lines.
Marine Natural Products Laboratory, Korea Ocean Research & Development Institute, Ansan, P O. Box 29, Seoul 425-600, Korea. shinhj@kordi.re.kr
Hee Jae
HJ
Shin
Hyun Sun
HS
Jeong
Hyi-Seung
HS
Lee
Song-Kyu
SK
Park
Hwan Mook
HM
Kim
Ho Jeong
HJ
Kwon
D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D002478:Cells, Cultured; D005591:Chemical Fractionation; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D006801:Humans; D007211:Indoles / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010080:Oxazoles / Q000302:isolation & purification / Q000494:pharmacology*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013302:Streptomyces / Q000737:chemistry* / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D000970:Antineoplastic Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007211:Indoles; D010080:Oxazoles; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C526070:streptochlorin