DNA polymerase recognition of 2'-deoxy-2'-fluoroarabinonucleoside 5'-triphosphates (2'F-araNTPs).
We examined the ability of 2'-deoxy-2'-fluroarabinonucleoside 5'-triphosphates (2'F-araNTPs) to serve as substrates of various DNA polymerases. In addition, we also examined the ability of these polymerases to accept DNA-FANA (2'-deoxy-2'-fluoroarabinonucleic acids) chimeras as template strands while synthesizing a DNA or FANA-DNA complementary strand. We provide preliminary data demonstrating that 2'F-araNTPs are indeed substrates of several DNA polymerases, and that FANA-DNA chimeric templates are generally well recognized by these polymerase enzymes.
Peng
Chang G
CG
McGill University, Montreal, Quebec, Canada.
Damha
Masad J
MJ
D001088:Arabinonucleotides / Q000737:chemistry* / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D052156:SELEX Aptamer Technique; D013379:Substrate Specificity
D001088:Arabinonucleotides; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase