[Research of real-time fluorescent PCR in the rapid differential detection of H5, H9, H7 subtype avian influenza inactivated vaccines].
Specific primers and TaqMan MGB probes were designed with Primer Express 2.0 software according to the conserved region of the H5, H9, H7 subtype AIV hemagglutinin gene to make research of real-time fluorescent one-step PCR in the differential detection of H5, H9, H7 subtype avian influenza inactivated vaccines. The result showed that the method was specific and reproducible. No cross-reaction was discovered with other avian disease vaccines. Real-time fluorescent PCR provided a specific, sensitive, rapid and convenient method for the subtype identification of avian influenza inactivated vaccines.
Key Laboratory of Veterinary Drug Innovation and Biosafety Evaluation, Ministry of Agriculture, China Institute of Veterinary Drug Control, Beijing 100081, China.
Jian-Feng
JF
Han
Yi-Bao
YB
Ning
Li
L
Song
Cheng-Huai
CH
Yang
D000818:Animals; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology; D053126:Influenza A Virus, H7N7 Subtype / Q000276:immunology; D053127:Influenza A Virus, H9N2 Subtype / Q000276:immunology; D009980:Influenza A virus / Q000145:classification* / Q000276:immunology*; D007252:Influenza Vaccines / Q000032:analysis* / Q000145:classification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D015164:Vaccines, Inactivated / Q000032:analysis
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated; C109963:hemagglutinin, avian influenza A virus