Crystallization and preliminary x-ray studies of the unliganded wild-type bovine thrombin.
Wild type of bovine thrombin has been crystallized in a ligand-free form by the hanging drop vapor diffusion method with polyethylene glycol 4000 and 2-propanol. The crystals belong to space group P4 3 2 12 with unit cell parameters of a = b = 87.7 A, c = 195.9 A. X-ray diffraction data were collected to 2.8 A resolution.
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, 061-0293, Japan.
Daisuke
D
Iyaguchi
Min
M
Yao
Nobuhisa
N
Watanabe
Isao
I
Tanaka
Eiko
E
Toyota
D000818:Animals; D002417:Cattle; D003460:Crystallization; D018360:Crystallography, X-Ray; D008024:Ligands; D016296:Mutagenesis; D012964:Sodium; D013917:Thrombin / Q000819:agonists / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics
D008024:Ligands; D012964:Sodium; D013917:Thrombin