Veillonella denticariosi sp. nov., isolated from human carious dentine.
Selective culture of human carious dentine for Veillonella strains resulted in the isolation of two strains of a Gram-negative, coccus-shaped bacterium that has not been described previously. Comparative 16S rRNA and dnaK gene sequence analysis indicated that the two strains were homogeneous and comprised a distinct lineage within the genus Veillonella, phylogenetically most closely related to Veillonella rodentium. This was supported by DNA-DNA hybridization, which showed clearly that the two strains were similar and distinct from other Veillonella species, and the production of major cellular fatty acids (C(13 : 0) and C(17 : 1)omega8), which is consistent with other members of the genus Veillonella. Based on these observations, strains RBV81 and RBV106(T) represent a novel species, for which the name Veillonella denticariosi sp. nov. is proposed, with the type strain RBV106(T) (=CIP 109448(T) =CCUG 54362(T) =DSM 19009(T)).
Byun
Roy
R
Institute of Dental Research, Westmead Centre for Oral Health and Westmead Millennium Institute, Westmead Hospital, Wentworthville, NSW 2145, Australia.
Carlier
Jean-Philippe
JP
Centre National de Référence des Bactéries Anaérobies et du Botulisme, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
Jacques
Nicholas A
NA
Institute of Dental Research, Westmead Centre for Oral Health and Westmead Millennium Institute, Westmead Hospital, Wentworthville, NSW 2145, Australia.
Marchandin
Helene
H
Centre Hospitalier Universitaire de Montpellier et EA 3755, Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 371 avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
Hunter
Neil
N
Institute of Dental Research, Westmead Centre for Oral Health and Westmead Millennium Institute, Westmead Hospital, Wentworthville, NSW 2145, Australia.
D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003731:Dental Caries / Q000382:microbiology*; D003804:Dentin / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D006801:Humans; D046529:Microscopy, Electron, Transmission; D018832:Molecular Chaperones / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014678:Veillonella / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D018832:Molecular Chaperones; D012336:RNA, Ribosomal, 16S