Electrochemical detection of kinase-catalyzed thiophosphorylation using gold nanoparticles.
An electrochemical biosensor for kinase-catalyzed reactions is coupled with the thiophosphorylation of the substrate peptide using adenosine 5'-[gamma-thio] triphosphate (ATP-S) as the co-substrate.
Department of Chemistry, University of Western Ontario, 1151 Richmond Street, London, Ontario N6A 5B7, Canada.
Kagan
K
Kerman
Heinz-Bernhard
HB
Kraatz
D002384:Catalysis; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry* / Q000378:metabolism*; D013696:Temperature
D010455:Peptides; D013438:Sulfhydryl Compounds; D006046:Gold; D011493:Protein Kinase C