[Relationship between alpha- and beta-isoform of corticosteroid receptors and corticosteroid resistant allergic rhinitis].
OBJECTIVE
To clarify the relationship between the expression of alpha- and beta-isoform of corticosteroid receptors (CS) in peripheral blood mononuclear cell (PBMC) and response to corticosteroid in patients with allergic rhinitis (AR).


METHODS
Semi-quantitative RT-PCR was used to detect the expression of CS-alpha, beta in PBMC in patients with AR and to observe the different responses to corticosteroid in controls. Immunocytochemical assay was used to detect the expression of protein of CS-alpha and CS-beta.


RESULTS
1) The expression of CS-alpha mRNA was detected in the sensitive group and the resistant group of patients with AR and the controls with CS-alpha/GAPDH mRNA (x +/- s) 1.15 +/- 0.75, 1.63 +/- 0.78, 1.27 +/- 0.51 respectively. 2) The expression of CS-beta mRNA in PBMC in the resistant group of patients with AR was significantly higher than that in the sensitive group and the controls (P < 0.05), with CS-beta/GAPDH mRNA 1.42 +/- 0.73, 0.82 +/- 0.59, 0.80 +/- 0.68 respectively. 3) The number of CS-beta-positive PBMC in the resistant group was significantly higher than that in the sensitive group and the controls (P < 0.01), with the number of CS-beta-positive PBMC 28.8% +/- 9. 9%, 5.9% +/- 3.2%, 5.5% +/- 6.8% respectively.


CONCLUSIONS
It is shown that the excessive expression of CS-beta may serve as a novel predictor of corticosteroid resistance in patients with AR.
Department of Otorhinolaryngology, Affiliated Xiangfan Hospital, Tongji Medical College, Huazhong University of Science and Technology, The Central Hospital of Xiangfan City, Xiangfan 441021, China. lh07103999370@163.com
Hong
H
Luo
Neng-bing
NB
Yan
Ping-fan
PF
Zeng
Jin-jun
JJ
Liang
Guo-hui
GH
Wu
Sai-xiong
SX
Ke
Peng-ju
PJ
Wang
Jin-yun
JY
Wang
D000293:Adolescent; D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D004351:Drug Resistance; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D020033:Protein Isoforms / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011987:Receptors, Steroid / Q000378:metabolism*; D012221:Rhinitis, Allergic, Perennial / Q000188:drug therapy / Q000378:metabolism*; D055815:Young Adult
D000305:Adrenal Cortex Hormones; D020033:Protein Isoforms; D012333:RNA, Messenger; D011987:Receptors, Steroid