Osteopontin expression of circulating T cells and plasma osteopontin levels are increased in relation to severity of heart failure.
BACKGROUND
T cells in peripheral blood reflect the systemic inflammatory response in patients with heart failure (HF). In a rat model of HF, osteopontin is dramatically increased in the left ventricular myocardium, so the association between osteopontin and HF was examined in the present study.


RESULTS
Peripheral blood was collected from 93 patients with heart disease and 38 controls. Left ventricular ejection fraction (LVEF) was calculated using a modified Simpson's rule. The 93 patients were classified into 3 classes according to the New York Heart Association (NYHA) functional classification. Osteopontin-expressing CD4+ T cells were quantified by flow cytometry. Plasma osteopontin levels (ng/ml) and the frequencies of osteopontin-expressing CD4+ T cells (%) were higher in patients with HF than in controls (800+/-554, 575+/-229, p=0.016 and 27.3+/-12.2, 16.7+/-10.0, p<0.001). Furthermore, the plasma osteopontin levels and the frequencies of osteopontin-expressing CD4+ T cells increased in proportion to the severity of the NYHA functional class. The frequencies of osteopontin-expressing CD4+ T cells were significantly correlated with LVEF (r=-0.336, p=0.0048) and log plasma brain natriuretic peptide levels (r=0.305, p=0.0025).


CONCLUSIONS
Osteopontin expression of circulating CD4+ T cells and plasma osteopontin levels reflect the severity of HF. Osteopontin could be a new target in the assessment of HF.
Soejima
Hirofumi
H
Department of Cardiovascular Medicine, Graduate School of Medical Sciences, Kumamoto University, Japan. yuuki@gpo.kumamoto-u.ac.jp
Irie
Atsushi
A
Fukunaga
Takashi
T
Oe
Yoko
Y
Kojima
Sunao
S
Kaikita
Koichi
K
Kawano
Hiroaki
H
Sugiyama
Seigo
S
Yoshimura
Michihiro
M
Kishikawa
Hideki
H
Nishimura
Yasuharu
Y
Ogawa
Hisao
H
D000368:Aged; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006333:Heart Failure / Q000097:blood* / Q000145:classification / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D053495:Osteopontin / Q000097:blood / Q000378:metabolism*; D011379:Prognosis; D012720:Severity of Illness Index; D013318:Stroke Volume / Q000502:physiology
D015415:Biomarkers; D053495:Osteopontin; D002097:C-Reactive Protein