Analysis of CoQ10 in rat serum by ultra-performance liquid chromatography mass spectrometry after oral administration.
A UPLC-MS method for determining Coenzyme Q(10) (CoQ(10)) levels in rat serum was developed. CoQ(10) was quantitatively extracted into 2-propanol using a fast extraction procedure. The separation of CoQ(10) was performed on a Waters Acquity UPLCtrade mark BEH C(18) column (1.7 microm, 1.0 mm x 50 mm) with the mobile phase containing acetonitrile, 2-propanol, and formic acid (90:10:0.1) over 5 min. The sensitivity of this method allows for the quantitation of 50 ng/mL CoQ(10) in serum (S/N=10). The linearity of this method was found to be from 50 to 20,000 ng/mL. The precision was less than 10% (intra- and inter-day), and the average extraction recovery was between 90 and 105%. This procedure provides a precise, sensitive and direct assay method for the determination of CoQ(10) in rat serum after oral administration. This method could be applied to further pharmacokinetic studies of CoQ(10).
Department of Pharmaceutics, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
Lie
L
Li
Deepthi
D
Pabbisetty
Paulo
P
Carvalho
Mitchell A
MA
Avery
Bonnie A
BA
Avery
D019840:2-Propanol / Q000737:chemistry; D000085:Acetates / Q000737:chemistry; D019342:Acetic Acid / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000284:Administration, Oral; D064753:Ammonium Hydroxide; D000818:Animals; D019540:Area Under Curve; D002853:Chromatography, Liquid / Q000379:methods*; D003067:Coenzymes / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics; D005561:Formates / Q000737:chemistry; D006878:Hydroxides / Q000737:chemistry; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D008657:Metabolic Clearance Rate; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics / Q000592:standards
D000085:Acetates; D000097:Acetonitriles; D003067:Coenzymes; D005561:Formates; D006878:Hydroxides; D012997:Solvents; C030544:formic acid; D014451:Ubiquinone; D064753:Ammonium Hydroxide; C024989:coenzyme Q10; C030571:ubiquinone 9; D019840:2-Propanol; D019342:Acetic Acid; C018824:ammonium acetate; C032159:acetonitrile