Expression profiles and pathway analysis in HEK 293 T cells overexpressing HIV-1 Tat and nucleocapsid using cDNA microarray.
Human immunodeficiency virus type 1 (HIV-1) infections are responsible for a substantial number of deaths annually and represent a significant threat to public health. According to the latest study, the Tat (Transactivator of transcription) protein is essential in transcription and replication of viral genes, and is among the early expression genes involved in the life cycle of HIV. The virion NC (nucleocapsid) plays an important role in early mRNA expression and contributes to the rapid viral replication that occurs during HIV-1 infection. Therefore, we attempted to elucidate the relationship between the Tat protein and nucleocapsid protein. In a comparison of two independently prepared and hybridized samples, flag NC overexpressed HEK 293T cells and pTat overexpressed HEK 293T cells, and hybridization showed the differences in expression in each case. Among the microarray results confirmed with real-time reverse transcriptase assay, twelve genes were identified to be involved according to their gene expression profiles. Of approximately 8,208 human genes that were analyzed, we monitored candidate genes that might have been related to NC and Tat genes from gene expression profiles. Additionally, the pathways could be viewed and analyzed through the use of PathwayStudio software. The pathways from the gene list were built and paths were found among the molecules/cell objects/processes by the curation method.
Department of Biological Science, Sookmyung Women's University Seoul 140-742, Korea.
Seong-Eun
SE
Park
Min Joo
MJ
Lee
Moon Hee
MH
Yang
Ka Young
KY
Ahn
Soo In
SI
Jang
Young Ju
YJ
Suh
Heejoon
H
Myung
Ji Chang
JC
You
Jong Hoon
JH
Park
D000465:Algorithms; D002460:Cell Line; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D015756:Genes, tat; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008954:Models, Biological; D019590:Nucleocapsid Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014162:Transfection
D019590:Nucleocapsid Proteins