Involvement of Escherichia coli K1 ibeT in bacterial adhesion that is associated with the entry into human brain microvascular endothelial cells.
IbeT is a downstream gene of the invasion determinant ibeA in the chromosome of a clinical isolate of Escherichia coli K1 strain RS218 (serotype 018:K1:H7). Both ibeT and ibeA are in the same operon. Our previous mutagenesis and complementation studies suggested that ibeT may coordinately contribute to E. coli K1 invasion with ibeA. An isogenic in-frame deletion mutant of ibeT has been made by chromosomal gene replacement with a recombinant suicide vector carrying a fragment with an ibeT internal deletion. The characteristics of the mutant in meningitic E. coli infection were examined in vitro [cell culture of human brain microvascular endothelial cells (HBMEC)] and in vivo (infant rat model of E. coli meningitis) in comparison with the parent strain. The ibeT deletion mutant was significantly less adhesive and invasive than its parent strain E. coli E44 in vitro, and the adhesion- and invasion-deficient phenotypes of the mutant can be complemented by the ibeT gene. Recombinant IbeT protein is able to block E. coli E44 invasion of HBMEC. Furthermore, the ibeT deletion mutant is less capable of colonizing intestine and less virulent in bacterial translocation across the blood-brain barrier (BBB) than its parent E. coli E44 in vivo. These data suggest that ibeT-mediated E. coli K1 adhesion is associated with the bacterial invasion process.
Saban Research Institute, Childrens Hospital Los Angeles, Los Angeles, CA, 90027, USA.
Yanming
Y
Zou
Lina
L
He
Feng
F
Chi
Ambrose
A
Jong
Sheng-He
SH
Huang
D000818:Animals; D001422:Bacterial Adhesion; D001812:Blood-Brain Barrier / Q000382:microbiology; D001921:Brain / Q000382:microbiology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005816:Genetic Complementation Test; D006801:Humans; D020814:Meningitis, Escherichia coli / Q000382:microbiology; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D037521:Virulence Factors