Placental stem cell markers in pre-eclampsia.
OBJECTIVE
To investigate the placental CD34, CD44, and leukemia inhibitory factor (LIF) levels in normotensive and pre-eclamptic women.


METHODS
The study population consisted of 21 women with pre-eclampsia. Twenty normotensive pregnant women served as controls and were matched to pre-eclamptic patients by gestational age at delivery. Decidual samples obtained from the central part of the placenta were stored at -70 degrees C until analysis. CD44 and LIF were quantified in homogenates by enzyme-linked immunosorbent assay (ELISA), while CD34 was quantified by flow cytometry.


RESULTS
The pre-eclamptic and normotensive groups were well matched. There were no significant differences in age, parity, weight, and gestational age at birth between the groups (P>0.05). The mean placental levels of CD34 (6.55+/-2.48 vs 3.16+/-1.23), CD44 (385.24+/-178.85 vs 157.75+/-31.73, and LIF (140+/-51.11 vs 96.25+/-31.62) were significantly higher in pre-eclamptic compared with normotensive women, respectively (P<0.05).


CONCLUSIONS
Higher levels of CD34, CD44, and LIF were found in the placentas of pre-eclamptic compared with normotensive women.
Department of Obstetrics and Gynecology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey. abenian@superonline.com
Ali
A
Benian
Hafize
H
Uzun
Seval
S
Aydin
Mustafa
M
Albayrak
Seyfettin
S
Uludağ
Riza
R
Madazli
D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D007231:Infant, Newborn; D053605:Leukemia Inhibitory Factor / Q000378:metabolism*; D010920:Placenta / Q000166:cytology / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D013234:Stem Cells / Q000378:metabolism*
D018952:Antigens, CD34; D015415:Biomarkers; D018960:Hyaluronan Receptors; D053605:Leukemia Inhibitory Factor