[Development and identification of the multiple B cell epitope antigens of Schistosoma japonicum].
OBJECTIVE
To develop multiple B cell epitope antigens of Schistosoma japonicum and evaluate their antigenicity.


METHODS
Bioinformatics software BioSun was used to predict B cell epitopes from Sj22.6, Sj14-3-3 and Sj26. The predicted epitopes P2, P6 and P7 were ligated to construct P2-P6-P7 and P6-P2-P7 multiepitope in random order, a 6 amino acid linker inserted between epitopes. Recombinant plasmids containing the two multiepitopes identified by enzyme digestion and sequencing were transformed into E. coli BL21. The expressed recombinant fusion proteins of E. coli BL21 induced with IPTG were purified with Ni2+ chelating HiTrap HP column. Their antigenicity was evaluated with Western-blotting.


RESULTS
The two multiple B cell epitopes P2-P6-P7 and P6-P2-P7 were successfully cloned into pET-32c(+) plasmid and fusion proteins were expressed. SDS-PAGE showed a single band and both of the recombinant fusion proteins were with Mr 20 400. The two proteins reacted with the sera of schistosomiasis patients but not with that of healthy people.


CONCLUSIONS
Two multiple B cell epitope antigens were developed with potential diagnosis value.
Zhang
Hui
H
Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China.
Zhu
Yin-chang
YC
Si
Jin
J
Zhao
Song
S
Wang
Xiao-ting
XT
Yin
Xu-ren
XR
Cao
Li-min
LM
Cao
Guo-qun
GQ
Hua
Wan-quan
WQ
Xu
Ming
M
Liang
You-sheng
YS
D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D006801:Humans; D011994:Recombinant Proteins / Q000276:immunology* / Q000302:isolation & purification; D012549:Schistosoma japonicum / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D012554:Schistosomiasis japonica / Q000097:blood / Q000469:parasitology
D000947:Antigens, Helminth; D018985:Epitopes, B-Lymphocyte; D011994:Recombinant Proteins