Increased levels of apoptosis in gastrocnemius skeletal muscle in patients with peripheral arterial disease.
Intermittent claudication (IC) is the major clinical manifestation of peripheral arterial disease (PAD). Apoptosis has been linked to skeletal muscle pathophysiology in other chronic diseases such as congestive heart failure. This study tested the hypothesis that there would be increased levels of apoptosis in the skeletal muscle of patients with PAD compared with control individuals. In total, 26 individuals with PAD and 28 age-appropriate controls underwent studies of peak oxygen consumption (peak VO2) and a gastrocnemius muscle biopsy in the most symptomatic leg. Muscle biopsies were analyzed for apoptosis and caspase-3 activity. Patients with PAD had a reduced peak VO2 compared with controls. Apoptosis was increased in those with PAD compared with age-appropriate controls (3.83% +/- 2.6 vs 1.53% +/- 0.96; p < 0.001). In conclusion, PAD is associated with increased levels of apoptosis in the peripheral skeletal muscle. Further study is required to ascertain whether apoptosis plays a role in decreased functional capacity.
Mitchell
Robert G
RG
Division of Cardiology, Department of Medicine, Durham VA and Duke University Medical Center, Durham, NC 27710, USA.
Duscha
Brian D
BD
Robbins
Jennifer L
JL
Redfern
Shelley I
SI
Chung
Jayer
J
Bensimhon
Daniel R
DR
Kraus
William E
WE
Hiatt
William R
WR
Regensteiner
Judith G
JG
Annex
Brian H
BH
D000368:Aged; D017209:Apoptosis; D016022:Case-Control Studies; D053148:Caspase 3 / Q000378:metabolism; D004789:Enzyme Activation; D005080:Exercise Test; D005260:Female; D006801:Humans; D007383:Intermittent Claudication / Q000209:etiology* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D010101:Oxygen Consumption; D016491:Peripheral Vascular Diseases / Q000150:complications / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D014481:United States; D016138:Walking
D053148:Caspase 3