The effects of nucleoside analogues on promoter methylation of selected tumor suppressor genes in MCF-7 and MDA-MB-231 breast cancer cell lines.
The effects of 2-chloro-2'-deoxyadenosine, 9-beta-D-arabinofuranosyl-2-fluoroadenine, and 5-aza-2'-deoxycytidine on promoter methylation of the selected tumor suppressor genes (i.e., ERalpha, BRCA1, RARbeta2, E-cadherin, PTEN, and APC) were estimated using methylation-sensitive restriction analysis. The studies were carried out in hormone-responsive, low-invasive cell line MCF-7 and hormone-insensitive, highly invasive cell line MDA-MB-231. The results demonstrate an implication of the tested adenosine analogues and 5-aza-dCyd in regulation of DNA methylation process. Moreover, the effects of nucleoside analogues on PTEN promoter methylation suggest distinct mechanism of regulation of the epigenetic DNA modification in low-invasive compared to highly invasive breast cancer cells.
Department of Biomedical Chemistry, Medical University of Lodz, Lodz, Poland. bkrawczyk@zdn.am.lodz.pl
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Krawczyk
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Rudnicka
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Fabianowska-Majewska
D000970:Antineoplastic Agents / Q000494:pharmacology; D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D017338:Cladribine / Q000494:pharmacology; D019175:DNA Methylation / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000077209:Decitabine; D005260:Female; D017491:Genes, APC / Q000187:drug effects; D019398:Genes, BRCA1 / Q000187:drug effects; D016147:Genes, Tumor Suppressor / Q000187:drug effects*; D006801:Humans; D009705:Nucleosides / Q000737:chemistry / Q000494:pharmacology*; D051059:PTEN Phosphohydrolase / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D018168:Receptors, Retinoic Acid / Q000235:genetics; D014740:Vidarabine / Q000031:analogs & derivatives / Q000494:pharmacology
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D009705:Nucleosides; D018168:Receptors, Retinoic Acid; C082925:retinoic acid receptor beta; D017338:Cladribine; D000077209:Decitabine; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; D014740:Vidarabine; D001374:Azacitidine; C024352:fludarabine