[The oral immune efficacy of recombinant lactobacillus casei expressing CSFV E290 peptide and it elicited specific CTL response].
The gene encoding classical swine fever virus (CSFV) T cell epitope E290 peptide was synthesized by PCR, cloned into the expression vector pPG-VP2 and named pPG-VP2-E290. The recombinant plasmid was electrotransformed into Lactobacillus casei 393 generating pPG-VP2-E290/L. casei 393. Specific anti-CSFV E290 peptide immunoglobulin G (IgG) antibody was detected by indirect ELISA in the serum of BALB/c mice and rabbits immunized with recombinant strain by oral administration. The CTL of E290 was analyzed with lymphocytes taken from the immunized mice, and the immunized rabbits were attacked with CSFV to validate the protective function of E290 antibody induced.


RESULTS
The recombinant expression system constructed with L. casei 393 in this study show a good immunization property and could elicit the mice and rabbits to produce high anti-E290 antibody levels. Furthermore, E290 peptide antibody could elicit specific CTL response, and restrain attack of CSFV to rabbits.
Xu
Yi-Gang
YG
Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Cui
Li-Chun
LC
Ge
Jun-Wei
JW
Zhao
Li-Li
LL
Li
Yi-Jing
YJ
D000284:Administration, Oral; D000818:Animals; D006691:Classical Swine Fever / Q000517:prevention & control; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D005260:Female; D007114:Immunization / Q000379:methods; D007780:Lacticaseibacillus casei / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015202:Protein Engineering; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013552:Swine; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D018984:Epitopes, T-Lymphocyte; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines