Reporter gene imaging using radiographic contrast from nonradioactive iodide sequestered by the sodium-iodide symporter.
The hypothesis that the human sodium-iodide symporter, NIS, can be used to detect NIS expression using standard radiological techniques was tested using adenoviral transduced NIS expression in human tumor xenografts grown in mice and in a naive dog prostate. Nonradioactive iodide was administered systemically to animals that 1-3 days previously had received a local injection of a replication-competent adenovirus expressing NIS under the control of the CMV promoter. The distribution of radiopacity was assessed in mouse tumors using micro-CT and a clinical X-ray machine and in the prostate of an anesthetized dog using a clinical spiral CT. Iodide sequestration and NIS expression were measured using X-ray spectrochemical analysis and fluorescence microscopy, respectively. Radiographic contrast due to NIS gene expression that was observed indicates the technique has potential for use in preclinical rodent tumor studies but probably lacks sensitivity for human use.
Department of Radiation Oncology, Henry Ford Health System, Detroit, MI 48202, USA. sbrown1@hfhs.org
Stephen L
SL
Brown
Svend O
SO
Freytag
Kenneth N
KN
Barton
Michael J
MJ
Flynn
Donald J
DJ
Peck
Aleksandar F
AF
Dragovic
Ryan
R
Jin
Yener N
YN
Yeni
David P
DP
Fyhrie
Clifford M
CM
Les
Guopei
G
Zhu
Andrew
A
Kolozsvary
Wayne C
WC
Pitchford
S David
SD
Nathanson
Joseph D
JD
Fenstermacher
Jae Ho
JH
Kim
D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000032:analysis* / Q000737:chemistry; D004285:Dogs; D017930:Genes, Reporter; D006801:Humans; D007455:Iodine / Q000032:analysis* / Q000737:chemistry; D008297:Male; D051379:Mice; D027981:Symporters / Q000235:genetics* / Q000378:metabolism; D014057:Tomography, X-Ray Computed; D000099209:Sodium-Iodide Symporters
D003287:Contrast Media; D007455:Iodine; D027981:Symporters; D000099209:Sodium-Iodide Symporters