Recombinant Mycobacterium bovis BCG expressing the LipL32 antigen of Leptospira interrogans protects hamsters from challenge.
The bacillus Calmette--Guerin (BCG), a live attenuated Mycobacterium bovis strain is considered a promising candidate as a vector system for delivery of foreign antigens to the immune system. The gene coding for the Leptospira interrogans external membrane protein LipL32, a highly immunogenic antigen found in all pathogenic leptospira, was cloned into several mycobacterial vectors for expression in BCG. Hamsters immunized with recombinant BCG (rBCG) expressing LipL32 were protected against mortality (P<or=0.05) upon challenge with a lethal inoculum of L. interrogans serovar Copenhageni. Autopsy examination did not reveal macroscopic or histological evidence of disease in rBCG immunized hamsters that survived lethal challenge. The data presented here further enhance the status of LipL32 as a promising candidate antigen for use in the control of leptospirosis, when presented to the immune system by an appropriate delivery system.
Seixas
Fabiana Kömmling
FK
Centro de Biotecnologia, Universidade Federal de Pelotas, Campus Universitário, 96010-900 Pelotas - RS, Brazil.
da Silva
Everton Fagonde
EF
Hartwig
Daiane Drawanz
DD
Cerqueira
Gustavo Maia
GM
Amaral
Marta
M
Fagundes
Michel Quevedo
MQ
Dossa
Robson Grando
RG
Dellagostin
Odir Antônio
OA
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D006224:Cricetinae; D005260:Female; D007114:Immunization; D007921:Leptospira interrogans / Q000276:immunology*; D007922:Leptospirosis / Q000473:pathology / Q000517:prevention & control; D008074:Lipoproteins / Q000235:genetics / Q000276:immunology*; D008297:Male; D008647:Mesocricetus; D009163:Mycobacterium bovis / Q000235:genetics*; D011994:Recombinant Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; C406589:LipL32 protein, Leptospira; D008074:Lipoproteins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic