Methermicoccus shengliensis gen. nov., sp. nov., a thermophilic, methylotrophic methanogen isolated from oil-production water, and proposal of Methermicoccaceae fam. nov.
A thermophilic, methylotrophic methanogen, strain ZC-1(T), was isolated from the Shengli oilfield, China. Cells of strain ZC-1(T) were motile cocci, 0.7-1.0 microm in diameter and always occurred in clusters of two to four cells. Lysis-susceptibility experiments and analysis of transmission electron micrographs of strain ZC-1(T) suggested the presence of a proteinaceous cell wall. Strain ZC-1(T) used methanol, methylamine and trimethylamine as substrates for methanogenesis. Optimal growth, with a doubling time of around 5 h, occurred at pH 6.0-6.5, 65 degrees C, 0.3-0.5 M NaCl and 0.05-0.20 M MgCl(2). The DNA G+C content of this organism was 56 mol%. Analysis of 16S rRNA gene sequence and the inferred amino acid sequence of the mcrA gene of strain ZC-1(T) indicated that it is related specifically to members of the family Methanosaetaceae (90.6 and 76.6 % sequence similarity, respectively). However, strain ZC-1(T) failed to grow with acetate as substrate for methanogenesis, which is a special characteristic of the family Methanosaetaceae. Based on these phenotypic and phylogenic characteristics, strain ZC-1(T) is proposed to represent a novel genus and species, for which the name Methermicoccus shengliensis gen. nov., sp. nov. is proposed. The type strain is ZC-1(T) (=CGMCC 1.5056(T)=DSM 18856(T)). Methermicoccaceae fam. nov. is also proposed.
Cheng
Lei
L
Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
Qiu
Tian-Lei
TL
Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
Yin
Xiao-Bo
XB
Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
Wu
Xiao-Lei
XL
Center for Environmental Biotechnology, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, PR China.
Hu
Guo-Quan
GQ
Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
Deng
Yu
Y
Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
Zhang
Hui
H
Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
D019342:Acetic Acid / Q000378:metabolism; D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001482:Base Composition; D002681:China; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D015636:Magnesium Chloride / Q000378:metabolism; D008697:Methane / Q000096:biosynthesis*; D000432:Methanol / Q000378:metabolism*; D019640:Methanosarcinales / Q000235:genetics; D008744:Methylamines / Q000378:metabolism; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D010578:Petroleum; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D014871:Water Microbiology
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D008744:Methylamines; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D015636:Magnesium Chloride; D012965:Sodium Chloride; C027451:methylamine; C023336:trimethylamine; D008697:Methane; D019342:Acetic Acid; D000432:Methanol