[Changes in electrophysical characteristics of Azospirillum brasilense Sp7 cells during their interaction with polyclonal antibodies].
Electrooptical characteristics of Azospirillum brasilense Sp7 cells during their specific interaction with polyclonal rabbit antibodies were studied. Dependence of optical density of cell suspension during electroorientation of cells from frequency of orienting field in interval 10, 100, 250, and 500 kHz was evaluated. Itwas shown that electrooptical (EO) characteristics of bacterial suspensions change during interaction of A. brasilense cells with antibodies, and maximal changes occur when frequency of oriented field amounts 100-250 kHz. During interaction of A. brasilense Sp7 with strain-specific polyclonal antibodies in the presence of Escherichia coli K-12 and Pseudomonas putida C-11 decrease of amplitude of analytic signal was observed but detection of A. brasilense Sp7 cells was possible. Possibility of detection of microorganisms by EO analysis during their interaction with antibodies was shown.
O I
OI
Guliĭ
V D
VD
Bunin
D
D
O'Neĭl
D
D
Ivnitskiĭ
L Iu
LIu
Matora
V V
VV
Ignatov
O V
OV
Ignatov
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000918:Antibody Specificity; D015806:Azospirillum brasilense / Q000302:isolation & purification / Q000502:physiology*; D015374:Biosensing Techniques / Q000379:methods*; D004560:Electricity; D004594:Electrophysiology; D055095:Optics and Photonics; D011817:Rabbits
D000907:Antibodies, Bacterial