[Dot enzyme-linked immunosorbent assay for detection of serum antibody to Blastocystis hominis in humans].
Serum and stool samples were collected from 322 undergraduate students in medical school. Using stool in vitro cultivation as golden standard, 178 cases were found Blastocystis hominis positive and 144 were negative. Dot-ELISA was used to examine the serum samples with a sensitivity of 92.1% (164/178) and specificity of 97.1% (141/144). This revealed that dot-ELISA can be used for antibody detection against Blastocystis hominis.
Gannan Medical College, Ganzhou 341000, China.
Shui-lian
SL
Su
Yi-ming
YM
Yan
Hua
H
Liao
Gui-feng
GF
Chen
Rui-qi
RQ
Zhang
Qiong-jun
QJ
Xie
Xiao
X
Le
Ya-qiong
YQ
Hu
Xue-ying
XY
Zeng
Hai-ying
HY
Lan
Rui-lian
RL
Xie
Zhen
Z
Huang
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D016776:Blastocystis Infections / Q000097:blood / Q000175:diagnosis / Q000469:parasitology*; D016845:Blastocystis hominis / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000469:parasitology; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000913:Antibodies, Protozoan