A two-strain mixture of rhizobacteria elicits induction of systemic resistance against Pseudomonas syringae and Cucumber mosaic virus coupled to promotion of plant growth on Arabidopsis thaliana.
We evaluated a commercial biopreparation of plant growth-promoting rhizobacteria (PGPR) strains Bacillus subtilis GB03 and B. amyloliquefaciens IN937a formulated with the carrier chitosan (BioYield) for its capacity to elicit growth promotion and induced systemic resistance against infection by Cucumber Mosaic Virus (CMV) and Pseudomonas syringae pv. tomato DC3000 in Arabidopsis thaliana. The biopreparation promoted plant growth of Arabidopsis hormonal mutants, which included auxin, gibberellic acid, ethylene, jasmonate, salicylic acid, and brassinosteroid insensitive lines as well as each wild-type. The biopreparation protected plants against CMV based on disease severity in wild-type plants. However, virus titre was not lower in control plants and those treated with biopreparation, suggesting that the biopreparation induced tolerance rather than resistance against CMV. Interestingly, the biopreparation induced resistance against CMV in NahG plants, as evidenced by both reduced disease severity and virus titer. The biopreparation also elicited induced resistance against P. syringae pv. tomato in the wild-type but not in NahG transgenic plants, which degrade endogenous salicylic acid, indicating the involvement of salicylic acid signaling. Our results indicate that some PGPR strains can elicit plant growth promotion by mechanisms that are different from known hormonal signaling pathways. In addition, the mechanism for elicitation of induced resistance by PGPR may be pathogen-dependent. Collectively, the two-Bacilli strain mixture can be utilized as a biological inoculant for both protection of plant against bacterial and viral pathogens and enhancement of plant growth.
Department of Entomology and Plant Pathology, Auburn University, Auburn, AL 36849, USA.
Choong-Min
CM
Ryu
John F
JF
Murphy
M S
MS
Reddy
Joseph W
JW
Kloepper
D017360:Arabidopsis / Q000254:growth & development* / Q000382:microbiology / Q000821:virology; D001407:Bacillus / Q000502:physiology*; D001412:Bacillus subtilis / Q000502:physiology*; D048271:Chitosan / Q000494:pharmacology; D017799:Cucumovirus / Q000472:pathogenicity*; D018517:Plant Roots / Q000382:microbiology*; D044224:Pseudomonas syringae / Q000472:pathogenicity*; D020156:Salicylic Acid / Q000494:pharmacology; D015398:Signal Transduction; D012988:Soil Microbiology
D048271:Chitosan; D020156:Salicylic Acid