Establishment and characterization of dual-species biofilms formed from a 3,5-dinitrobenzoic-degrading strain and bacteria with high biofilm-forming capabilities.
In this study, the possibility of establishing a dual-species biofilm from a bacterium with a high biofilm-forming capability and a 3,5-dinitrobenzoic acid (3,5-DNBA)-degrading bacterium, Comamonas testosteroni A3, was investigated. Our results showed that the combinations of strain A3 with each of five strains with a high biofilm-forming capability (Pseudomonas sp. M8, Pseudomonas putida M9, Bacillus cereus M19, Pseudomonas plecoglossicida M21 and Aeromonas hydrophila M22) presented different levels of enhancement regarding biofilm-forming capability. Among these culture combinations, the 24-h dual-species biofilms established by C. testosteroni A3 with P. putida M9 and A. hydrophila M22 showed the strongest resistance to 3,5-DNBA shock loading, as demonstrated by six successive replacements with DMM2 synthetic wastewater. The degradation rates of 3,5-DNBA by these two culture combinations reached 63.3-91.6% and 70.7-89.4%, respectively, within 6 h of every replacement. Using the gfp-tagged strain M22 and confocal laser scanning microscopy, the immobilization of A3 cells in the dual-species biofilm was confirmed. We thus demonstrated that, during wastewater treatment processes, it is possible to immobilize degrader bacteria with bacteria with a high biofilm-forming capability and to enable them to develop into the mixed microbial flora. This may be a simple and economical method that represents a novel strategy for effective bioaugmentation.
College of Life Sciences, Nanjing Agricultural University, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing, China.
Mengying
M
Li
Lu
L
Peng
Zhang
Z
Ji
Jingliang
J
Xu
Shunpeng
S
Li
D016980:Aeromonas hydrophila; D001409:Bacillus cereus / Q000502:physiology; D001419:Bacteria / Q000302:isolation & purification / Q000378:metabolism*; D018407:Bacterial Physiological Phenomena; D001673:Biodegradation, Environmental; D018441:Biofilms / Q000254:growth & development*; D020604:Comamonas testosteroni / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000032:analysis; D009579:Nitrobenzoates / Q000378:metabolism*; D011549:Pseudomonas / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000032:analysis; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D009579:Nitrobenzoates; D011993:Recombinant Fusion Proteins; D014874:Water Pollutants, Chemical; D049452:Green Fluorescent Proteins; C026017:3,5-dinitrobenzoic acid