Identification of DNA basepairing via tunnel-current decay.
We propose a new approach for reading the sequence of a DNA molecule passing between electrodes on a nanopore, using hydrogen bond-mediated tunneling signals. The base-electrode interaction is modeled using a nucleo-base-functionalized STM probe that is pulled away from a nucleoside monolayer. Watson-Crick recognition results in slow decay of the tunnel current, which is uniquely characteristic of the basepair in over half the reads. Thirteen independent reads would yield the desired 99.99% accuracy.
He
Jin
J
Biodesign Institute, Department of Chemistry and Biochemistry, Department of Physics, Arizona State University, Tempe, AZ 85287, USA.
Lin
Lisha
L
Zhang
Peiming
P
Lindsay
Stuart
S
D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D003841:Deoxycytidine / Q000737:chemistry; D016252:Microscopy, Scanning Tunneling / Q000379:methods; D008958:Models, Molecular; D013936:Thymidine / Q000737:chemistry
D003841:Deoxycytidine; D004247:DNA; D013936:Thymidine