Codeine accumulation and elimination in larvae, pupae, and imago of the blowfly Lucilia sericata and effects on its development.
The aim of this study was to evaluate the reliability of insect larvae as samples for toxicological investigations. For this purpose, larvae of Lucilia sericata were reared on samples of minced pig liver treated with different concentrations of codeine: therapeutic, toxic, and potentially lethal doses. Codeine was detected in all tested larvae, confirming the reliability of these specimens for qualitative toxicology analysis. Furthermore, concentrations measured in larvae were correlated with levels in liver tissue. These observations bring new elements regarding the potential use of opiates concentrations in larvae for estimation of drug levels in human tissues. Morphine and norcodeine, two codeine metabolites, have been also detected at different concentrations depending on the concentration of codeine in pig liver and depending on the substance itself. The effects of codeine on the development of L. sericata were also investigated. Results showed that a 29-h interval bias on the evaluation of the larval stage duration calculated from the larvae weight has to be considered if codeine was present in the larvae substrate. Similarly, a 21-h interval bias on the total duration of development, from egg to imago, has to be considered if codeine was present in the larvae substrate.
Kharbouche
Hicham
H
Institut Universitaire de Médecine Légale, Centre Hospitalier Universitaire Vaudois and University of Lausanne, rue du Bugnon 21, CH-1005 Lausanne, Switzerland. Hicham.Kharbouche@chuv.ch
Augsburger
Marc
M
Cherix
Daniel
D
Sporkert
Frank
F
Giroud
Christian
C
Wyss
Claude
C
Champod
Christophe
C
Mangin
Patrice
P
D000818:Animals; D003061:Codeine / Q000031:analogs & derivatives / Q000032:analysis* / Q000633:toxicity; D004175:Diptera / Q000737:chemistry*; D053593:Forensic Toxicology; D008401:Gas Chromatography-Mass Spectrometry; D007814:Larva / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D009020:Morphine / Q000032:analysis; D009294:Narcotics / Q000032:analysis* / Q000633:toxicity; D011679:Pupa / Q000737:chemistry; D013552:Swine
D009294:Narcotics; C010414:norcodeine; D009020:Morphine; D003061:Codeine