Fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea.
To elucidate fungal diversity in methane hydrate-bearing deep-sea marine sediments in the South China Sea, internal transcribed spacer (ITS) regions of rRNA genes from five different sediment DNA samples were amplified and phylogenetically analyzed. Total five ITS libraries were constructed and 413 clones selected randomly were grouped into 24 restriction patterns by Amplified Ribosomal DNA Restriction Analysis (ARDRA). ITS sequences of 44 representative clones were determined and compared with the GenBank database using gapped-BLAST. The phylogenetic analysis showed that the ITS sequences (71-97% similarity) were similar to those of Phoma, Lodderomyces, Malassezia, Cryptococcus, Cylindrocarpon, Hortaea, Pichia, Aspergillus and Candida. The remaining sequences were not associated to any known fungi or fungal sequences in the public database. The results suggested that methane hydrate-bearing deep-sea marine sediments harbor diverse fungi. This is the first report on fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea.
State Key Laboratory for Biocontrol and Department of Biochemistry, College of Life Sciences, Zhongshan (Sun Yatsen) University, Guangzhou, People's Republic of China.
Xintian
X
Lai
Lixiang
L
Cao
Hongming
H
Tan
Shu
S
Fang
Yali
Y
Huang
Shining
S
Zhou
D002681:China; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D017753:Ecosystem; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D008697:Methane / Q000737:chemistry*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D021903:DNA, Ribosomal Spacer; D008697:Methane