Adhesion of staphylococcal and Caco-2 cells on diamond-like carbon polymer hybrid coating.
Staphylococci cause the majority of the nosocomial implant-related infections initiated by adhesion of planktonic bacteria to the implant surface. It was hypothesized that plasma accelerating filtered pulsed arc discharge method enables combination of the advantageous properties of diamond with the antisoiling properties of polymers. Diamond-like carbon polytetrafluoroethylene hybrid (DLC-PTFE-h) coating was produced. The adhesion of S. aureus ATCC 25923 (10(8) colony-forming units/mL) to surfaces diminished from 2.32%, 2.35%, and 2.57% of high quality DLC, titanium, and oxidized silicon, respectively, to 1.93% of DLC-PTFE-h. For S. epidermidis ATCC 35984 the corresponding figures were 3.90%, 3.32%, 3.47%, and 2.57%. Differences in bacterial adhesion between recombinant DLC-PTFE-h and other materials were statistically significant (p < 0.05). In contrast, human Caco-2 cells adhered as well to DLC-PTFE-h as to DLC, titanium, or silicon, which were all in the MTT test found to be cytocompatible. DLC-PTFE-h coating can be used to modify the surface properties of any surgical implants and is an unfavorable substrate for staphylococcal cells, but compatible with human Caco-2 cells. DLC-PTFE-h coating may help in the combat against Staphylococcus-related implant infections which usually require both antibiotics and surgical removal of the implant for cure.
Kinnari
Teemu J
TJ
Department of Clinical Microbiology, Fundación Jiménez Díaz-UTE, Avda. de Reyes Catolicos 2, ES-28040 Madrid, Spain. teemu.j.kinnari@helsinki.fi
Soininen
Antti
A
Esteban
Jaime
J
Zamora
Nieves
N
Alakoski
Esa
E
Kouri
Vesa-Petteri
VP
Lappalainen
Reijo
R
Konttinen
Yrjö T
YT
Gomez-Barrena
Enrique
E
Tiainen
Veli-Matti
VM
D001422:Bacterial Adhesion; D018938:Caco-2 Cells; D002244:Carbon / Q000378:metabolism*; D002448:Cell Adhesion; D002470:Cell Survival; D020099:Coated Materials, Biocompatible / Q000378:metabolism*; D018130:Diamond / Q000378:metabolism*; D006801:Humans; D011108:Polymers / Q000378:metabolism*; D012825:Silicon; D013211:Staphylococcus aureus / Q000166:cytology*
D020099:Coated Materials, Biocompatible; D011108:Polymers; D002244:Carbon; D018130:Diamond; D012825:Silicon