Meganuclease and transposon mediated transgenesis in medaka.
From among a plethora of various gene delivery methods, the researcher must choose the right one according to availability for a given species and the precise application the transgenic animal is intended for. Here we review the progress in meganuclease and Sleeping Beauty transposon mediated transgenesis over recent years with a focus on medaka and zebrafish. We present a side-by-side comparison of these two approaches based on their biologic properties and provide interesting perspectives for future experiments and applications, which are different for the two techniques because of their distinct modes of action.
Pediatric Oncology, Dana-Farber Cancer Institute, Binney St, Boston, Massachusetts 02115, USA. Clemens_grabher@dfci.harvard.edu
Clemens
C
Grabher
Joachim
J
Wittbrodt
D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D018014:Gene Transfer Techniques; D009990:Oryzias / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
D004251:DNA Transposable Elements; D029701:Saccharomyces cerevisiae Proteins; C037725:SCEI protein, S cerevisiae; D015252:Deoxyribonucleases, Type II Site-Specific