Effect of two different bioabsorbable collagen membranes on guided bone regeneration: a comparative histomorphometric study in the dog mandible.
BACKGROUND
This study compared bone regeneration following guided bone regeneration with two bioabsorbable collagen membranes in saddle-type bone defects in dog mandibles.


METHODS
Three standardized defects were created, filled with bone chips and deproteinized bovine bone mineral (DBBM), and covered by three different methods: control = no membrane; test 1 = collagen membrane; and test 2 = cross-linked collagen membrane (CCM). Each side of the mandible was allocated to one of two healing periods (8 or 16 weeks). The histomorphometric analysis assessed the percentage of bone, soft tissue, and DBBM in the regenerate; the absolute area in square millimeters of the bone regenerate; and the distance in millimeters from the bottom of the defect to the highest point of the regenerate.


RESULTS
In the 8-week healing group, two dehiscences occurred with CCM. After 8 weeks, all treatment modalities showed no significant differences in the percentage of bone regenerate. After 16 weeks, the percentage of bone had increased for all treatment modalities without significant differences. For all groups, the defect fill height increased between weeks 8 and 16. The CCM group showed a statistically significant (P = 0.0202) increase over time and the highest value of all treatment modalities after 16 weeks of healing,


CONCLUSIONS
The CCM showed a limited beneficial effect on bone regeneration in membrane-protected defects in dog mandibles when healing was uneventful. The observed premature membrane exposures resulted in severely compromised amounts of bone regenerate. This increased complication rate with CCM requires a more detailed preclinical and clinical examination before any clinical recommendations can be made.
Bornstein
Michael M
MM
Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, Bern, Switzerland. michael.bornstein@zmk.unibe.ch
Bosshardt
Dieter
D
Buser
Daniel
D
D020341:Absorbable Implants; D016301:Alveolar Bone Loss / Q000601:surgery*; D000818:Animals; D001861:Bone Regeneration; D018786:Bone Substitutes; D002417:Cattle; D003094:Collagen; D003432:Cross-Linking Reagents / Q000009:adverse effects; D004285:Dogs; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D008567:Membranes, Artificial; D011446:Prospective Studies; D011897:Random Allocation; D013529:Surgical Wound Dehiscence / Q000209:etiology; D013552:Swine
D018786:Bone Substitutes; D003432:Cross-Linking Reagents; D008567:Membranes, Artificial; D003094:Collagen