Relationship between plasma apolipoproteinB concentrations, apolipoproteinB/apolipoproeinA-I and HDL subclasses distribution.
BACKGROUND
To analyze the relationship between plasma apoB100 concentrations, apoB100/apoA-I ratio and the alteration of HDL subclasses distribution.


METHODS
The apoA-I contents of plasma HDL subclasses were quantitated by 2-dimensional gel electrophoresis coupled with immunodetection in 506 subjects.


RESULTS
The subjects in the highest tertile of apoB100 groups had significantly higher small-sized prebeta(1)-HDL, however lower large-sized HDL(2a), HDL(2b) than the subjects in the lowest tertile of apoB100 group. Furthermore, a significant down in the HDL(2b)/prebeta(1)-HDL values (from 6.8 to 1.6) with a rise in apoB100/apoA-I ratio (from 0.4 to 1.4) were observed. Compared to subjects with apoB100/apoA-I ratio<0.9, the subjects with apoB100/apoA-I ratio > or =0.9 had significantly higher small-sized prebeta(1)-HDL whereas lower HDL(3a), HDL(3b) and large-sized HDL(2a), HDL(2b.) Pearson correlation revealed that concentrations of apoB100 were positively correlated with prebeta(1)-HDL but negatively correlated with HDL(2a) and HDL(2b), and in multivariate analysis, all HDL subclasses were independently associated with the apoB100/apoA-I ratio.


CONCLUSIONS
The apoB100 concentrations, especially apoB100/apoA-I ratio could reflect sensitively the alteration of HDL subclasses distribution. And HDL subclasses distribution characteristics of hyperlipidemic subjects appeared in the subjects with apoB100/apoA-I ratio > or =0.9, which indicated the efficiency of RCT was weakened and the maturation of HDL was blocked.
Apolipoprotein Research Unit, Department of Biochemistry and Molecular Biology, West China Medical School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, PR China.
Li
L
Tian
Xinwei
X
Wu
Mingde
M
Fu
Yang
Y
Qin
Yanhua
Y
Xu
Lianqun
L
Jia
D016632:Apolipoprotein A-I / Q000097:blood*; D001055:Apolipoproteins B / Q000097:blood*; D008076:Cholesterol, HDL / Q000097:blood* / Q000145:classification*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D014280:Triglycerides / Q000097:blood
D016632:Apolipoprotein A-I; D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D014280:Triglycerides