Construction of a small-molecule-integrated semisynthetic split intein for in vivo protein ligation.
A new split intein-based protein ligation tool that is synthetically accessible and can be used for protein semisynthesis on the cell surface and potentially inside cells has been constructed.
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
Tomomi
T
Ando
Shinya
S
Tsukiji
Tsutomu
T
Tanaka
Teruyuki
T
Nagamune
D047668:Inteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019154:Protein Splicing; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D011506:Proteins