Bactericidal effect of TiO2 on the selected Vibrio parahaemolyticus and optimization using response surface methodology.
In this work, the bactericidal effect of TiO2 on selected typical food pathogenic bacteria, Vibrio parahaemolyticus was studied. V parahaemolyticus is an important pathogen of humans and aqua-cultured animals. We established the response surface methodology (Box-Behnken Design) to investigate the effect of principal parameters on the cell sterilization such as TiO2 concentration, UV illumination time, temperature, and pH. The sterilization rate reached maximum value at the TiO2 concentration of 1.0 mg/ml. During irradiation under the time of 30 min with UV light with the 1g-TiO2/l, the sterilization rate was greater than 85%, and 99% or more cell lost their viability with 3 hours of irradiation. Sterilization rate of the cell increased with decrease in the pH and temperature.
Department of Environmental Engineering, Chonnam National University, Gwangju 500-757, Korea.
Tae-Young
TY
Kim
Kyung-Hee
KH
Park
Seon-Gyun
SG
Rho
Seung-Jai
SJ
Kim
Sung-Young
SY
Cho
D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D020650:Combinatorial Chemistry Techniques; D003198:Computer Simulation; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D008954:Models, Biological; D013242:Sterilization / Q000379:methods*; D013499:Surface Properties; D014025:Titanium / Q000008:administration & dosage* / Q000737:chemistry; D014466:Ultraviolet Rays; D014736:Vibrio parahaemolyticus / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects*
D000900:Anti-Bacterial Agents; C009495:titanium dioxide; D014025:Titanium