Photodynamic effect on melanoma cells investigated by atomic force microscopy.
Atomic force microscopy (AFM) is a modern experimental method for imaging of conducting or non-conducting samples. New trends in the application of scanning probe microscopy (SPM) give us the ability to scan live cells directly in their ingenuous surroundings or in air. Our apparatus was replenished with an inverse optical microscope, so we could observe the position of the scanning tip in every individual cell. The aim of the presented study is to picture the cell surface in air. A dry scanner in non-contact or tapping mode was used in the biological application of AFM. In our work the cell line G361 was used as a biological sample. We imaged the cell line before and after induction of a photodynamic effect (PDE) by irradiation of ZnTPPS4-loaded cells with a light dose of 15 J/cm(2). Individual cells before PDE induction had a smooth surface without protrusion on the entire surface. Cells after PDE induction did not have a smooth surface but their surface was rough with protrusion and in some places cleaved.
Department of Medical Biophysics, Faculty of Medicine, Palacky University in Olomouc, Hnevotínská 3, 775 15 Olomouc, Czech Republic. tomanko@tunw.upol.cz
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Tomankova
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Kolarova
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Vujtek
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Bajgar
D045744:Cell Line, Tumor; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D008545:Melanoma / Q000188:drug therapy* / Q000648:ultrastructure*; D008665:Metalloporphyrins / Q000627:therapeutic use*; D018625:Microscopy, Atomic Force / Q000379:methods*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000627:therapeutic use
D008665:Metalloporphyrins; D017319:Photosensitizing Agents; C474512:zinc-tetrakis(p-sulfonatophenyl)porphyrin