Cohnella laeviribosi sp. nov., isolated from a volcanic pond.
A novel thermophilic and endospore-forming Gram-positive bacterium capable of assimilating and isomerizing l-ribose was isolated from a volcanic area in Likupang, Indonesia. The isolate, RI-39(T), was able to grow at high temperatures (37-60 degrees C); optimum growth was observed at pH 6.5 and 45 degrees C. The strain contained MK-7 (87 %) as the main respiratory quinone and had a DNA G+C content of 51 mol%. The major cellular fatty acids of the isolate were iso-C(16 : 0) and anteiso-C(15 : 0) and the predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysyl-phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate represents an evolutionary lineage that is distinct from those of other Cohnella species. Based on morphological, physiological and chemotaxonomic characteristics and 16S rRNA gene sequence comparisons, it is proposed that strain RI-39(T) represents a novel species, Cohnella laeviribosi sp. nov. The type strain is RI-39(T) (=KCTC 3987(T) =KCCM 10653P(T) =CCUG 52217(T)).
Cho
Eun-Ah
EA
Department of Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea.
Lee
Jung-Sook
JS
Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yusong-gu, Daejeon 305-806, Republic of Korea.
Lee
Keun Chul
KC
Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yusong-gu, Daejeon 305-806, Republic of Korea.
Jung
Heung-Chae
HC
Systems Microbiology Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yusong-gu, Daejeon 305-806, Republic of Korea.|GenoFocus Inc., Yusong, Daejeon 305-811, Republic of Korea.
Pan
Jae-Gu
JG
Systems Microbiology Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yusong-gu, Daejeon 305-806, Republic of Korea.|GenoFocus Inc., Yusong, Daejeon 305-811, Republic of Korea.
Pyun
Yu-Ryang
YR
Department of Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D016936:Gram-Positive Endospore-Forming Bacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007214:Indonesia; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012266:Ribose / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012266:Ribose