Molecular characterization reveals that YMR278w encoded protein is environmental stress response homologue of Saccharomyces cerevisiae PGM2.
The uncharacterized ORF YMR278w of Saccharomyces cerevisiae is a member of D-phosphohexomutase super family, annotated as phosphoribomutase. In order to evaluate its functional role, we cloned, over-expressed and purified YMR278w protein. The protein product of YMR278w exhibits phosphoglucomutase activity. S158T mutant derivative of YMR278w protein lost phosphoglucomutase activity. Purified YMR278w protein has higher K(m) for glucose-1-phosphate compared to other known phosphoglucomutases. Trehalose content was reduced in YMR278w disruptant as compared to the wild type strain. Based on the above results we suggest that YMR278w encodes phosphoglucomutase and not phosphoribomutase.
Tiwari
Anurag
A
Laboratory of Molecular Genetics, School of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
Bhat
Jayadeva Paike
JP
D000595:Amino Acid Sequence; D004789:Enzyme Activation; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010733:Phosphoglucomutase / Q000737:chemistry* / Q000378:metabolism*; D010770:Phosphotransferases / Q000737:chemistry* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D006360:Heat-Shock Proteins; D010770:Phosphotransferases; C036179:phosphoribomutase; D010733:Phosphoglucomutase