Two threonine residues required for role of AfsKav in controlling morphogenesis and avermectin production in Streptomyces avermitilis.
AfsKav is a eukaryotic-type serine/threonine protein kinase, required for sporulation and avermectin production in Streptomyces avermitilis. In terms of their ability to complement SJW4001 (DeltaafsK-av), afsK-av mutants T165A and T168A were not functional, whereas mutants T165D and T168D retained their ability, indicating that Thr-165 and Thr-168 are the phosphorylation sites required for the role of AfsKav. Expression of the S-adenosylmethione synthetase gene promoted avermectin production in the wild-type S. avermitilis, yet not in the mutant harboring T168D or T165D, demonstrating that tandem phosphorylation on Thr-165 and Thr-168 in AfsKav is the mechanism modulating avermectin production in response to S-adenosylmethione accumulation in S. avermitilis.
Department of Biological Science, Institute of Bioscience and Biotechnology, Myongji University, Yongin 449-728, Korea.
Arishma
A
Rajkarnikar
Hyung-Jin
HJ
Kwon
Yeon-Woo
YW
Ryu
Joo-Won
JW
Suh
D015964:Gene Expression Regulation, Bacterial; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000378:metabolism; D009024:Morphogenesis / Q000502:physiology*; D009154:Mutation / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012436:S-Adenosylmethionine / Q000378:metabolism; D013302:Streptomyces / Q000166:cytology / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D013912:Threonine / Q000502:physiology*
D013912:Threonine; D007559:Ivermectin; C019264:avermectin; D012436:S-Adenosylmethionine; D017346:Protein Serine-Threonine Kinases