In vitro evaluation of platelet concentrates during storage: Platelet counts and markers of platelet destruction.
BACKGROUND
Differences in platelet counts are observed by use of automated haematology analyzers making interlaboratory comparison difficult.


METHODS
Twenty-eight single-donor platelet concentrates (PCs) were collected. Platelet concentration and markers of platelet destruction were investigated during storage for 11/12 days.


RESULTS
Increasing impedance-immunoplatelet ratio was observed during storage, correlating to platelet fragments, large platelets, platelet density and cell-lysis. High variability was observed for optical-immunoplatelet ratio.


CONCLUSIONS
Immunoplatelet count or correction factor calculated by impedance-immunoplatelet ratio should be used to confirm that platelet unit meets platelet count requirements or to compare results from clinical trials. Optical platelet count is not recommended.
Apelseth
Torunn O
TO
Blood Bank, Haukeland University Hospital, N-5021 Bergen, Norway. torunn.oveland.apelseth@helse-bergen.no
Hervig
Tor
T
D001792:Blood Platelets / Q000166:cytology*; D017097:Electric Impedance; D006801:Humans; D010976:Platelet Count / Q000379:methods*; D011309:Preservation, Biological; D013997:Time Factors