In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography.
We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in opaque tissues. We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time.
Vinegoni
Claudio
C
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th Street, Charlestown, Massachusetts 02129, USA. cvinegoni@mgh.harvard.edu
Pitsouli
Chrysoula
C
Razansky
Daniel
D
Perrimon
Norbert
N
Ntziachristos
Vasilis
V
D000818:Animals; D004331:Drosophila melanogaster / Q000166:cytology*; D007089:Image Enhancement / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008029:Lighting / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D011679:Pupa / Q000166:cytology; D041622:Tomography, Optical / Q000379:methods*