Proteomic analysis of pancreas derived from adult cloned pig.
The potential medical applications of animal cloning include xenotransplantation, but the complex molecular cascades that control porcine organ development are not fully understood. Still, it has become apparent that organs derived from cloned pigs may be suitable for transplantation into humans. In this study, we examined the pancreas of an adult cloned pig developed through somatic cell nuclear transfer (SCNT) using two-dimensional electrophoresis (2-DE) and Western blotting. Proteomic analysis revealed 69 differentially regulated proteins, including such apoptosis-related species as annexins, lamins, and heat shock proteins, which were unanimously upregulated in the SCNT sample. Among the downregulated proteins in SCNT pancreas were peroxiredoxins and catalase. Western blot results indicate that several antioxidant enzymes and the anti-apoptotic protein were downregulated in SCNT pancreas, whereas several caspases were upregulated. Together, these data suggest that the accumulation of reactive oxygen species (ROS) in the pancreas of an adult cloned pig leads to apoptosis.
Chae
Jung-Il
JI
Centre for Regenerative Medicine, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 111 Gwahangno, Yuseong-gu, Daejeon 305-806, Republic of Korea.
Cho
Young Keun
YK
Cho
Seong-Keun
SK
Kim
Jin-Hoi
JH
Han
Yong-Mahn
YM
Koo
Deog-Bon
DB
Lee
Kyung-Kwang
KK
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D019976:Cloning, Organism / Q000379:methods*; D020869:Gene Expression Profiling; D010179:Pancreas / Q000378:metabolism*; D020543:Proteome / Q000235:genetics*; D013552:Swine / Q000235:genetics*
D020543:Proteome