The influence of repeated blood withdrawals before surgery on clinical outcome.
The aim of this study was to find the influence of blood withdrawals and diet iron on elective surgery. Male Wistar rats (n=24) were divided: 1. group (SLD) ate standard laboratory diet (SLD), 2. group (FE) an iron enriched diet (FE) with one blood withdrawal after 9 weeks. 3. group (SLD-w) SLD and 4. group (FE-w) ate the FE diet; with 9 withdrawals once a week. The rats were sacrificed 18 hour after partial hepatectomy (PH) in the 10th week. Liver DNA synthesis (3H-thymidin - kBq/mg DNA) was performed. Serum hepcidin (pg/ml), iron concentration, respiratory burst of polymorfonucleares (RB, spontaneous; stimulated, %), count of blood cells were determined. FE-w had a higher (2.36+/-0.36) liver DNA synthesis after PH vs. SLD (1.21+/-0.49). Higher hemoglobin in erythrocytes (pg) was in FE-w and SLD-w vs. FE and SLD. PMN count in SLD-w, FE-w increased vs. SLD, FE. Hepcidin after PH decreased in SLD (78.0), FE (68.0), FE-w (97.0), but increased in SLD-w (217). Serum iron increased in SLD-w. RB after PH increased in FE-w (4.5; 47.6) vs. SLD (1.15; 29.1), FE (3.20;17.8), SLD-w (3.30;13.7).


CONCLUSIONS
The iron diet with stimulation of haematopoesis by withdrawals improves an organism's condition expressed as better response to elective surgery and better PMN functions.
Radio-Isotope Laboratory and Vivarium, Charles University in Prague, Faculty of Medicine, University Hospital in Hradec Králové, Czech Republic. zivna@lfhk.cuni.cz
Helena
H
Zivná
Pavel
P
Zivný
Vladimír
V
Palicka
Eva
E
Simáková
Vít
V
Rehácek
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000097:blood; D001804:Blood Transfusion, Autologous; D006410:Hematopoiesis; D006498:Hepatectomy; D064451:Hepcidins; D007501:Iron / Q000378:metabolism; D019266:Iron, Dietary / Q000008:administration & dosage; D008099:Liver / Q000378:metabolism; D008297:Male; D009504:Neutrophils / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D016897:Respiratory Burst; D013514:Surgical Procedures, Operative
D023181:Antimicrobial Cationic Peptides; C578703:Hamp protein, rat; D064451:Hepcidins; D019266:Iron, Dietary; D007501:Iron