[Mycoplasma genitalium lipid-associated membrane proteins induce human monocytic cell express proinflammatory cytokines and apoptosis by activating nuclear factor kappaB].
Designed to investigate the potential pathogenicity of Mycoplasma genitalium (M. genitalium) and its molecular mechanisms responsible for the induction of proinflammatory cytokines gene expression in human monocytic cells (THP-1) stimulated by lipid-associated membrane proteins (LAMPs) prepared from M. genitalium. THP-1 cells were stimulated with LAMPs to analyze the production of proinflammatory cytokines and the expression of mRNA was detected by RT-PCR. Cell apoptosis was detected in THP-1 cells by Annexin V-propidium iodide staining. The activity of transcriptional factors, NF-kappaB, was examined in THP-1 cells treated with LAMPs by EMSA. The effects of pyrrolidine dithiocarbamate (PDTC), an inhibitor of NF-kappaB, on the production of proinflammatory cytokines, the expression of mRNA and apoptosis were also examined in THP-1 cells treated with LAMPs. M. genitalium LAMPs stimulate THP-1 cells to produce TNF-alpha, IL-1beta and IL-6 in dose- and time-dependent manner. The mRNA levels and cell apoptosis are also downregulated in response to LAMPs stimulation and inhibited by PDTC treatment. M. genitalium LAMPs are found to trigger NF-kappaB activation, a possible mechanism for the induction of mRNA expression and the cell apoptosis. This study demonstrated that M. genitalium may be an important etiological factor of certain disease due to the ability of LAMPs to stimulated the expression of mRNA and apoptosis, which is probably mediated through the activation of NF-kappaB.
Qiu
Hong
H
Department of Microbiology, Medical College, Nanhua University, Hengyang 421001, China. qiuh_1979@163.com
Wu
Yi-Mou
YM
You
Xiao-Xing
XX
Yu
Ming-Jun
MJ
Zeng
Yan-Hua
YH
D017209:Apoptosis / Q000187:drug effects*; D001426:Bacterial Proteins / Q000633:toxicity*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008055:Lipids; D008565:Membrane Proteins / Q000633:toxicity*; D009000:Monocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D045704:Mycoplasma genitalium / Q000472:pathogenicity*; D016328:NF-kappa B / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis
D001426:Bacterial Proteins; D016207:Cytokines; D008055:Lipids; D008565:Membrane Proteins; D016328:NF-kappa B; D012333:RNA, Messenger