Do secondary compounds inhibit microbial- and insect-mediated leaf breakdown in a tropical rainforest stream, Costa Rica?
We examined the hypothesis that high concentrations of secondary compounds in leaf litter of some tropical riparian tree species decrease leaf breakdown by inhibiting microbial and insect colonization. We measured leaf breakdown rates, chemical changes, bacterial, fungal, and insect biomass on litterbags of eight species of common riparian trees incubated in a lowland stream in Costa Rica. The eight species spanned a wide range of litter quality due to varying concentrations of nutrients, structural and secondary compounds. Leaf breakdown rates were fast, ranging from 0.198 d(-1 )(Trema integerrima) to 0.011 d(-1) (Zygia longifolia). Processing of individual chemical constituents was also rapid: cellulose was processed threefold faster and hemicellulose was processed fourfold faster compared to similar studies in temperate streams. Leaf toughness (r = -0.86, P = 0.01) and cellulose (r = -0.78, P = 0.02) were the physicochemical parameters most strongly correlated with breakdown rate. Contrary to our initial hypothesis, secondary compounds were rapidly leached (threefold faster than in temperate studies), with all species losing all secondary compounds within the first week of incubation. Cellulose was more important than secondary compounds in inhibiting breakdown. Levels of fungal and bacterial biomass were strongly correlated with breakdown rate (fungi r = 0.64, P = 0.05; bacteria r = 0.93, P < 0.001) and changes in structural compounds (lignin r = -0.55, P = 0.01). Collector-gatherers were the dominant functional group of insects colonizing litterbags, in contrast to temperate studies where insect shredders dominate. Insect biomass was negatively correlated with breakdown rate (r = -0.70, P = 0.02), suggesting that insects did not play an important role in breakdown. Despite a wide range of initial concentrations of secondary compounds among the eight species used, we found that secondary compounds were rapidly leached and were less important than structural compounds in determining breakdown rates.
Ardón
Marcelo
M
Institute of Ecology, University of Georgia, Athens, GA 30602, USA. mla5@duke.edu
Pringle
Catherine M
CM
D000818:Animals; D018533:Biomass; D003364:Costa Rica; D004602:Elements; D007313:Insecta; D008031:Lignin / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry* / Q000382:microbiology / Q000469:parasitology; D045483:Rivers / Q000382:microbiology / Q000469:parasitology; D013634:Tannins / Q000032:analysis*
D004602:Elements; D010636:Phenols; D013634:Tannins; D008031:Lignin