Use of 120-nm deep channels for liquid chromatographic separations.
The present study reports on the exploration of the separation speed limits of RPLC chromatography in open-tubular channels. Applying the shear-driven chromatography principle in a 120-nm deep open channel, and using an improved detection set-up, the separation of three coumarin dyes was detectable 8mm downstream of the injection point. At this distance, separation efficiencies of N = 17,900 - 24,100 plates were obtained at a velocity of 10 mm s(-1), corresponding to a plate generation velocity of 21,100 to 28,300 plates per second for the most and least retained component, respectively.
Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium.
Veronika
V
Fekete
David
D
Clicq
Wim
W
De Malsche
Han
H
Gardeniers
Gert
G
Desmet
D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D008962:Models, Theoretical