Methylobacterium platani sp. nov., isolated from a leaf of the tree Platanus orientalis.
A novel bacterial strain, designated PMB02(T), was isolated from a leaf of the tree Platanus orientalis. Colonies grown on TYG agar plates were circular, pink-pigmented and slow-growing, being 0.2-1.5 mm in diameter after 3 days growth. The cells of strain PMB02(T) were Gram-negative, aerobic, motile rods that possessed oxidase and catalase activities and grew at 20-30 degrees C, pH 6-8 and in media containing less than 1 % NaCl. The major respiratory quinone was identified as Q-10. A phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain PMB02(T) was related to members of the genus Methylobacterium. A comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium aquaticum and Methylobacterium variabile, with which it showed sequence similarities of 97.7 and 97.4 %, respectively. The values for DNA-DNA hybridization between strain PMB02(T) and M. aquaticum CCM 7218(T) and M. variabile GR3(T) were less than 32 %. On the basis of the phenotypic characterization, the phylogenetic analysis and the DNA-DNA relatedness data, strain PMB02(T) is considered to represent a novel species of the genus Methylobacterium, for which the name Methylobacterium platani sp. nov. is proposed. The type strain is PMB02(T) (=KCTC 12901(T)=JCM 14648(T)).
Kang
Yoon-Suk
YS
Division of Environmental Science and Ecological Engineering, Korea University, Seoul, South Korea.
Kim
Juhyun
J
Division of Environmental Science and Ecological Engineering, Korea University, Seoul, South Korea.
Shin
Hyeon-Dong
HD
Division of Environmental Science and Ecological Engineering, Korea University, Seoul, South Korea.
Nam
Young-Do
YD
Biological Resource Center, KRIBB, Daejeon, South Korea.
Bae
Jin-Woo
JW
Biological Resource Center, KRIBB, Daejeon, South Korea.
Jeon
Che Ok
CO
Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, South Korea.
Park
Woojun
W
Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, South Korea.|Division of Environmental Science and Ecological Engineering, Korea University, Seoul, South Korea.
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D002374:Catalase / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D019684:Magnoliopsida / Q000382:microbiology*; D020580:Methylobacterium / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D018515:Plant Leaves / Q000382:microbiology*; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D010088:Oxidoreductases; D002374:Catalase