Lysophosphatidic acid-induced interleukin-1 beta expression is mediated through Gi/Rho and the generation of reactive oxygen species in macrophages.
Lysophosphatidic acid (LPA), a low-molecular-weight lysophospholipid enriched in platelets and mildly oxidized low-density lipoproteins, is known to regulate inflammation and atherosclerosis by binding to its cognate receptors. In this study, we reported that LPA upregulated interleukin-1 beta (IL-1 beta) expression in mouse J774A.1 macrophages. By using pharmacological inhibitors, it was suggested that G(i)/Rho activation and subsequent reactive oxygen species (ROS) production were involved in IL-1 beta induction. In addition, IL-1 beta induction by LPA was also observed in human primary macrophages. In summary, LPA is involved in the processes of inflammation by affecting macrophage behavior.
Institute of Zoology, National Taiwan University, Taipei, Taiwan.
Chi-Lun
CL
Chang
Mu-En
ME
Lin
Hsien-Yeh
HY
Hsu
Chao-Ling
CL
Yao
Shiaw-Min
SM
Hwang
Chien-Yuan
CY
Pan
Chi-Yen
CY
Hsu
Hsinyu
H
Lee
D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000502:physiology*; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism*; D008246:Lysophospholipids / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D017931:DNA Primers; D053583:Interleukin-1beta; D008246:Lysophospholipids; D017382:Reactive Oxygen Species; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; C032881:lysophosphatidic acid