[Potential use of the herbicide quizalofop-p-ethyl for eicosapentaenoic acid overproduction by the diatom Nitzschia laevis].
The diatom Nitzschia laevis is a good alternative source of eicosapentaenoic acid (EPA). Besides strategies for high cell density culture, EPA productivity may be further improved by herbicides. The effect of the herbicide quizalofop-p-ethyl on the growth and EPA production was studied in this paper. As the solvent of the herbicide, DMSO was proved to inhibit the growth and EPA production of N. laevis. The concentration of DMSO in the medium should not exceed 0.2%. Quizalofop-p-ethyl could cause morphology damage to the N. laevis cells. With the increasing concentration of quizalofop-p-ethyl from 0 mmol/L to 0.4 mmol/L, the dry cell weight production decreased, while at the same time, the lipid content of the dry cell mass increased. When treated with 0.1 mmol/L quizalofop-p-ethyl, the EPA content increased from 3.00% to 3.58% (of dry cell weight, DW), and the proportion of EPA (20:5) in total fatty acids (TFA) increased from 25.15% to 32.88% . These results indicated that the herbicide quizalofop-p-ethyl could stimulate the accumulation of EPA; therefore it might be useful for selecting algae colonies that overproduce EPA.
Cao
Xiao-Hong
XH
Tuuyin Key Laboratory of Food Nutrition and Safety, College of Food Science and Biotechnology, Tianoin University of Science and Technology, Tianjin 300457, China. caoxh@tust.edu.cn
Li
Song-Yao
SY
Wang
Chun-Ling
CL
Lu
Mei-Fang
MF
D003470:Culture Media; D046508:Culture Techniques; D017377:Diatoms / Q000254:growth & development / Q000378:metabolism*; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis*; D006540:Herbicides / Q000494:pharmacology; D011422:Propionates / Q000494:pharmacology*; D011810:Quinoxalines / Q000494:pharmacology*
D003470:Culture Media; D006540:Herbicides; D011422:Propionates; D011810:Quinoxalines; C064835:quizalofop-ethyl; D015118:Eicosapentaenoic Acid