An alternative approach for the synthesis of fluorogenic substrates of endo-beta-(1-->4)-xylanases and some applications.
Fluorogenic substrates of endo-beta-(1-->4)-xylanases (EXs), 4-methylumbelliferyl beta-glycosides of xylobiose and xylotriose were synthesized from fully acetylated oligosaccharides using the alpha-trichloroacetimidate procedure. A commercially available syrup containing xylose and xylo-oligosaccharides was used as the starting material. Both fluorogenic glycosides were found to be suitable substrates for EXs, particularly for sensitive detection of the enzymes in electrophoretic gels and their in situ localization on sections of fruiting bodies of some plants, such as tomato, potato and eggplant, all of the family Solanaceae.
Vrsanská
Mária
M
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538 Bratislava, Slovakia.
Nerinckx
Wim
W
Claeyssens
Marc
M
Biely
Peter
P
D004187:Disaccharides / Q000138:chemical synthesis; D004586:Electrophoresis; D043364:Endo-1,4-beta Xylanases / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D010944:Plants / Q000201:enzymology; D014312:Trisaccharides / Q000138:chemical synthesis; D014994:Xylose
D004187:Disaccharides; D005456:Fluorescent Dyes; D014312:Trisaccharides; C515044:xylotriose; D014994:Xylose; D043364:Endo-1,4-beta Xylanases; C004173:xylobiose