Characterization of typo-, regio-, and stereo-selectivities of babaco latex lipase in aqueous and organic media.
The unripe fruit of babaco (Vasconcellea x heilbornii; syn. Carica pentagona) contains a latex, similar to that in Carica papaya, which exhibits lipolytic activity. Herein, the regioselectivity, stereoselectivity and typoselectivity in both hydrolysis and acyltransfer reactions of babaco latex lipases were studied and compared to those of Carica papaya latex. In hydrolysis, both biocatalysts are 1,3-regioselective with ratios for 1,2-2,3-diacylglycerols/1,3-diacylglycerol of 6.5 and 21 for babaco and papaya, respectively. In contrast, papaya latex had a slight sn-3 stereopreference. Babaco latex displayed a higher activity on triacylglycerols with short chain and unsaturated fatty acids.
Cambon
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UMR IATE, Laboratoire de lipotechnie, CIRAD, TA 40/16, 73 rue JF Breton, 34398 Montpellier cedex 5, France.
Rodriguez
J A
JA
Pina
M
M
Arondel
V
V
Carriere
F
F
Turon
F
F
Ruales
J
J
Villeneuve
P
P
D029441:Carica / Q000201:enzymology; D031241:Caricaceae / Q000201:enzymology*; D002849:Chromatography, Gas; D004075:Diglycerides / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D007840:Latex / Q000378:metabolism*; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism
D004075:Diglycerides; D007840:Latex; D010940:Plant Proteins; D008049:Lipase