[Effects of salvianolic acid B on cerebral energy charge and activity of ATPase in mice with cerebral ischemia].
OBJECTIVE
To observe the effect of salvianolic acid B (SalB) on high energy phosphate and activity of ATPase of cerebral ischemia in mice, and to study the role of SalB on hydrocephalus further.


METHODS
NIH mice were divided into four groups randomly: Sham-operated group, cerebral ischemia group, SalB-treated group and Nimodipine (Nim)-collated group. In Sal B-treated group, mice were injected with SalB (22.5 mg x kg(-1)) in vena caudalis at 30 min before the experiment. In Nim-collated group, Nim (0.03 mg x kg(-1)) was injected into tail vein at the same time, while the mice in Sham-operated group and cerebral ischemia group were injected the same volume normal saline. The acute cerebral ischemia model was established by ligating bilateral common carotid arteries for 30 min in mice, then the mice were killed and the content of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), phosphocreatine (PCr) were observed, and the cerebral energy charge (EC) was computed. At the same time, activity of Na(+) -K(+) -ATPase and Ca2(+) -ATPase, content of water in brain tissue were measured.


RESULTS
Compared with cerebral ischemia group, EC and content of ATP, ADP, PCr in SalB-treated group heightened evidently (P < 0.01). Moreover, activity of Na(+)-K+ ATPase and Ca2+ ATPase in SalB-treated group had a remarkable increase (P < 0.01). But the content of water in brain tissue decreased markedly (P < 0.05).


CONCLUSIONS
The mechanism that SalB can relieve content of water in brain tissue of cerebral ischemia in mice, may be associated with improving the content of high-energy phosphoric acid compounds and enhancing the activity of ATPase.
Jiang
Yu-Feng
YF
Department of Pathology, Beijing University of Chinese Medicine, Beijing 100029, China. jiangyf66@yahoo.com.cn
Wang
Qiu-Hua
QH
Liu
Zhi-Qin
ZQ
Wang
Qian
Q
Cai
Da-Yong
DY
Liu
Shi-Jing
SJ
Zhang
Ying
Y
Huang
Qi-Fu
QF
D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002545:Brain Ischemia / Q000503:physiopathology*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects*; D008297:Male; D051379:Mice; D010725:Phosphocreatine / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D027561:Salvia miltiorrhiza / Q000737:chemistry; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014867:Water / Q000378:metabolism; D053630:Depsides
D000244:Adenosine Diphosphate; D000249:Adenosine Monophosphate; D000251:Adenosine Triphosphatases; D000255:Adenosine Triphosphate; D001572:Benzofurans; D000252:Calcium-Transporting ATPases; D010725:Phosphocreatine; D000254:Sodium-Potassium-Exchanging ATPase; D014867:Water; C076944:salvianolic acid B; D053630:Depsides