[Effect of LIPUS on the cellular behavior of human primary macrophages].
OBJECTIVE
To explore the effect of low intensity pulsed ultrasound (LIPUS) on the biological behavior of macrophages to provide some theoretical foundations for its clinical application.


METHODS
Human primary macrophages were obtained by using modified Ficoll-paque density gradient centrifugation combined with adherent method. The phagocytosis of macrophages was analyzed using fluorescein-labelled E.coli. The expression of the secreted proteins was determined by proteins array and the secretion of metalloproteinases (MMPs) was examined by gelatin zymography. The focal complexes and extracellular matrix metalloproteinase inducer (CD147) were detected by fluorescence microscopy and the phosphorylation of Src and ERKs was examined by Western blot.


RESULTS
The human primary macrophages were successfully isolated and cultured in vitro. LIPUS accelerated the macrophages to phagocytose E.coli, promoted the protein expression of CD147 and MMPs, increased the level of protein tyrosine phosphorylation, and induced the formation of focal complexes and the phosphorylation of Src and ERKs.


CONCLUSIONS
LIPUS may up-regulate the phagocytosis and secretion of human primary macrophages through the activation of Src and ERKs.
Li
Yi-Ping
YP
Department of Pathology, Basic Medical School, Southeast University, Nanjing 210009, China. lypzzm@yahoo.com.cn
Zhou
Shao-Xia
SX
Andreas
Schmelz
S
Max
G Bachem
GB
D000818:Animals; D051926:Basigin / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008264:Macrophages / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism; D010587:Phagocytosis; D010766:Phosphorylation; D014464:Ultrasonic Therapy; D014465:Ultrasonics
D016207:Cytokines; D051926:Basigin; D020782:Matrix Metalloproteinases