Low-density lipoprotein protects Vibrio vulnificus-induced lethality through blocking lipopolysaccharide action.
Lipoprotein plays a role in the host defense against bacterial infection, and its serum level has been demonstrated to be an important prognosis factor of survival. We have previously demonstrated that LDL directly inactivates the hemolytic activity of Vibrio vulnificus cytolysin (VVC) in vitro. The object of this study was therefore to examine whether the LDL-mediated inactivation of VVC leads to protection against lethal infection of V. vulnificus in vivo, using wild and VVC-deficient V. vulnificus strains. Unexpectedly, we found that LDL protects mouse lethality induced by VVC-deficient as well as wild V. vulnificus strain. We also demonstrated that LDL blocks V. vulnificus LPS-induced lethality in mice. These results suggest that LDL preferentially act on endotoxin rather than exotoxin in the protection against V. vulnificus-induced mice lethality.
Park
Kwang Hyun
KH
College of Pharmacy, Medicinal Resources Research Institute, Wonkwang University, Iksan 570-749, Korea.
Kim
Jong Suk
JS
Lee
Young Rae
YR
Moon
Young Jae
YJ
Hur
Hyun
H
Choi
Yun Hee
YH
Kim
Cheon Hyeon
CH
Kim
Uh Hyun
UH
Song
Eun Kyung
EK
Yoo
Wan Hee
WH
Lee
Chang Seop
CS
Kim
Byeong Soo
BS
Lee
Sung Ho
SH
Ryu
Phil Youl
PY
Han
Myung Kwan
MK
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors*; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D054353:Perforin / Q000037:antagonists & inhibitors / Q000235:genetics; D014735:Vibrio Infections / Q000517:prevention & control; D041261:Vibrio vulnificus / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity*; D014774:Virulence / Q000187:drug effects / Q000235:genetics / Q000502:physiology
D008070:Lipopolysaccharides; D008077:Lipoproteins, LDL; D054353:Perforin