Direct neurite-osteoblastic cell communication, as demonstrated by use of an in vitro co-culture system.
Using an in vitro co-culture approach comprising cultured murine superior cervical ganglia and MC3T3-E1 osteoblast-like cells, we found that the addition of scorpion venom (SV) elicited neurite activation via intracellular Ca2+ mobilization and, after a lag period, osteoblastic Ca2+ mobilization. SV did not have any direct effect on the osteoblastic cells in the absence of neurites. The addition of an alpha1-adrenergic receptor (AR) antagonist, prazosin, dose-dependently prevented the osteoblastic activation that resulted as a consequence of neural activation by SV. These results demonstrate that osteoblastic activation occurred as a direct response to neuronal activation, which activation was mediated by alpha1-ARs in the osteoblastic cells.
Obata
Koji
K
Department of Pharmacology, School of Dentistry, Aichi-Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan.
Furuno
Tadahide
T
Nakanishi
Mamoru
M
Togari
Akifumi
A
D000322:Adrenergic Agonists / Q000494:pharmacology; D018674:Adrenergic Antagonists / Q000494:pharmacology; D000814:Aniline Compounds; D000818:Animals; D020013:Calcium Signaling / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D002460:Cell Line; D018920:Coculture Techniques / Q000379:methods*; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D016501:Neurites / Q000187:drug effects / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011941:Receptors, Adrenergic / Q000235:genetics / Q000378:metabolism; D018013:Receptors, Neuropeptide / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D012604:Scorpion Venoms / Q000494:pharmacology; D017783:Superior Cervical Ganglion / Q000166:cytology / Q000187:drug effects; D014966:Xanthenes
D000322:Adrenergic Agonists; D018674:Adrenergic Antagonists; D000814:Aniline Compounds; D009479:Neuropeptides; D012333:RNA, Messenger; D011941:Receptors, Adrenergic; D018013:Receptors, Neuropeptide; D012604:Scorpion Venoms; D014966:Xanthenes; C059715:Fluo-3