Cloning and phylogenetic analysis of the chitinase gene from the facultative pathogen Paecilomyces lilacinus.
OBJECTIVE
To PCR-amplify the full-length genomic-encoding sequence for one chitinase from the facultative fungal pathogen Paecilomyces lilacinus, analyse the DNA and deduced amino acid sequences and compare the amino acid sequence with chitinases reported from mycopathogens, entomopathogens and nematopathogens.


RESULTS
The encoding gene (designated as PLC) was isolated using the degenerate PCR primers and the DNA-Walking method. The gene is 1458 bp in length and contains three putative introns. A number of sequence motifs that might play a role in its regulation and function had also been found. Alignment of the translation product (designated as Plc, molecular mass of 45.783 kDa and pI of 5.65) with homologous sequences from other species showed that Plc belongs to Class V chitinase within the glycosyl hydrolase family 18. The phylogenetic and molecular evolutionary analysis using mega (Molecular Evolutionary Genetics Analysis) indicated that these chitinases from mycopathogens, entomopathogens and nematopathogens, the majority of which belong to glycosyl hydrolase family 18, were clustered into two well-supported subgroups corresponding to ascomycetes fungal and nonfungal chitinases (bacteria, baculoviruses).


CONCLUSIONS
Our study showed that chitinases from mycoparasitic, entomopathogenic and nematophagous fungi are closely related to each other and reaffirmed the hypothesis that baculovirus chitinase is most likely to be of a bacterial origin - acquired by gene transfer. Bacterial and baculoviral chitinases in our study are potential pathogenicity factors; however, we still cannot ascribe any specific function to those chitinases from the fungi.


CONCLUSIONS
To our knowledge, this is the first report describing the chitinase gene and its translation product from Paecilomyces lilacinus, which constitutes the largest number of formulated biological nematicides reported so far, this is also the first study to analyse and resolve the phylogenetic and molecular evolutionary relationships among the chitinases produced by mycopathogens, entomopathogens and nematopathogens.
Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan Province, China.
L Q
LQ
Dong
J K
JK
Yang
K Q
KQ
Zhang
D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D002688:Chitinases / Q000145:classification / Q000235:genetics*; D003001:Cloning, Molecular; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D009348:Nematoda / Q000235:genetics; D010143:Paecilomyces / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D012988:Soil Microbiology
D002688:Chitinases