Jasmonates and its mimics differentially elicit systemic defence responses in Nicotiana attenuata.
Coronalon (6-ethyl indanoyl isoleucine), a synthetic jasmonate mimic, is known to regulate levels of transcripts and secondary metabolites that are commonly elicited by methyl jasmonate (MeJA) in a variety of plants. The ability of coronalon and its derivative (In-L-Ile-Me) to elicit MeJA-activated transcriptional and defence responses [nicotine and trypsin proteinase inhibitors (TPIs)] was compared in treated and systemic untreated tissues of wild-type (WT) and NaLOX3-silenced Nicotiana attenuata plants which are unable to activate either local or systemic defence responses. Coronalon and its derivative significantly regulated 71% and 86% of genes up-regulated by MeJA and 53% and 66% of the genes down-regulated by MeJA in the treated leaves, but only 3% and 7% of all regulated genes in untreated, but phylotactically connected, leaves of WT plants. Consistent with their ability to elicit transcriptional responses in treated tissues, coronalon and In-L-Ile-Me increased nicotine and TPIs when applied to the tissues in which these metabolites are produced, namely roots and leaves. However, treating roots elicited TPI activity in leaves in both WT and NaLOX3-silenced plants, suggesting that mimics can be transported apoplastically from roots to leaves in the xylem. This response was lower in NaLOX3-silenced plants, suggesting that the ability of coronalon and In-L-Ile-Me to elicit TPI responses in leaves after root treatments requires intact jasmonic acid (JA) signalling. Treating leaves did not elicit detectable changes in endogenous JA levels but did decrease free salicylic acid contents. It is concluded that coronalon and In-L-Ile-Me elicit jasmonate responses in treated tissues and could be valuable tools for dissecting local and systemic jasmonate signalling networks in plants.
Pluskota
Wioletta E
WE
Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Str. 8, D-07745 Jena, Germany.
Qu
Nan
N
Maitrejean
Mathias
M
Boland
Wilhelm
W
Baldwin
Ian T
IT
D000085:Acetates / Q000494:pharmacology*; D003517:Cyclopentanes / Q000378:metabolism / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D020868:Gene Silencing; D054884:Host-Pathogen Interactions / Q000187:drug effects*; D007189:Indans / Q000494:pharmacology; D007532:Isoleucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D054883:Oxylipins / Q000378:metabolism / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism; D014026:Nicotiana / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
D000085:Acetates; D003517:Cyclopentanes; D007189:Indans; C528224:N-((1-oxoindan-4-yl)carbonyl)isoleucine methyl ester; D054883:Oxylipins; D010937:Plant Growth Regulators; C484935:coronalon; D007532:Isoleucine; C011006:jasmonic acid; C072239:methyl jasmonate; D020156:Salicylic Acid