The repeated immobilization stress increases IL-1beta immunoreactivities in only neuron, but not astrocyte or microglia in hippocampal CA1 region, striatum and paraventricular nucleus.
The effect of repeated immobilization stress (RIS) on the expression of interleukin-1beta (IL-1beta) and types of cells that express IL-1beta in hippocampal CA1 region, striatum and paraventricular nucleus (PVN) were investigated in ICR mice. The RIS was induced daily for 2h for 4 consecutive days. In the immunohistochemical study, RIS increased IL-1beta immunoreactivities (IR) in the hippocampal CA1 region and striatum and PVN. The RIS also increased glial fibrillary acidic protein (GFAP) IR and complement receptor type 3 (OX-42) IR in the hippocampal CA1 regions and striatum but not PVN. In confocal immunofluorescence study, the IL-1beta IR increased by RIS were colocalized with only NeuN, but not GFAP or OX-42 in the hippocampal CA1 region, striatum and PVN. Our results indicate that RIS increases IL-1beta IR on neuron, but not astrocyte or microglia in the hippocampal CA1 region, striatum and PVN, suggesting that the IL-1beta IR on neuron may play an important role during RIS. In addition, GFAP and OX-42 increased by RIS may be involved indirectly in playing another role in the hippocampal CA1 region and striatum during RIS.
Kwon
Min-Soo
MS
Department of Pharmacology, College of Medicine, and Institute of Natural Medicine, Hallym University, 1 Okcheondong, Chuncheon, Gangwon-Do 200-702, South Korea.
Seo
Young-Jun
YJ
Lee
Jin-Koo
JK
Lee
Han-Kyu
HK
Jung
Jun-Sub
JS
Jang
Jeong-Eun
JE
Park
Soo-Hyun
SH
Suh
Hong-Won
HW
D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D039481:CD11b Antigen / Q000032:analysis / Q000378:metabolism; D002908:Chronic Disease; D003342:Corpus Striatum / Q000378:metabolism / Q000503:physiopathology; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism / Q000503:physiopathology; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D017628:Microglia / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism / Q000503:physiopathology; D016548:Prosencephalon / Q000378:metabolism* / Q000503:physiopathology; D012149:Restraint, Physical / Q000523:psychology; D013315:Stress, Psychological / Q000378:metabolism* / Q000503:physiopathology; D015854:Up-Regulation / Q000502:physiology*
D015415:Biomarkers; D039481:CD11b Antigen; D005904:Glial Fibrillary Acidic Protein; C509295:ITGAM protein, human; D053583:Interleukin-1beta