Two novel aminooligosaccharides isolated from the culture of Streptomyces coelicoflavus ZG0656 as potent inhibitors of alpha-amylase.
Two novel aminooligosaccharides were separated from the culture filtrate of Streptomyces coelicoflavus ZG0656. Their chemical structures were determined by electrospray ionization tandem mass spectrometry (ESI-MS/MS) and 2D nuclear magnetic resonance (NMR) spectroscopy. Because of their acarviosine core structures, the names acarviostatins II23 and II13 were given to the novel compounds. The two acarviostatins were both mixed noncompetitive inhibitors of porcine pancreatic alpha-amylase (PPA), with inhibition constants (K(i)) of 0.009 microM (acarviostatin II23) and 0.010 microM (acarviostatin II13). Therefore, acarviostatin II23 and acarviostatin II13 are, respectively, 231 and 208 times more potent than acarbose.
Department of Microbiology, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin 300071, People's Republic of China.
Peng
P
Geng
Gang
G
Bai
D000606:Amino Sugars / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D013302:Streptomyces / Q000737:chemistry*; D013552:Swine; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
D000606:Amino Sugars; D004791:Enzyme Inhibitors; D009844:Oligosaccharides; D000516:alpha-Amylases