Quantification of fenitrothion and its main metabolites in poplar leaves by isotope dilution gas chromatography-mass spectrometry coupled with solid-phase microextraction.
A sensitive and specific method for determining fenitrothion and its main metabolites, 3-methyl-4-nitrophenol and fenitrooxon, in poplar leaves using deuterated isotopes as the internal standard is described. The analytes and the labeled isotopes were extracted from leaves by solid-phase microextraction and subsequently analysed by gas chromatography coupled with mass spectrometry. The method had a chromatographic run time of 17.0 min and good linearity over the range 0.01-10 mg kg(-1). The detection limits ranged between 2.5 and 0.6 microg kg(-1). The isotopic dilution technique allowed improving significantly the repetitivity even using different fibers with the same coating (RSD<5.1%). The method was applied successfully to study the persistence of fenitrothion in forestal matrices in a poplar forest after cannon spray application of the insecticide.
Department of Analytical Chemistry, Faculty of Pharmacy, University of the Basque Country, 01006 Vitoria-Gasteiz, Spain.
Alicia
A
Sánchez
Silvia
S
Millán
M Carmen
MC
Sampedro
Nora
N
Unceta
Esther
E
Rodríguez
M Aranzazu
MA
Goicolea
Ramón J
RJ
Barrio
D005278:Fenitrothion / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007202:Indicators and Reagents; D010575:Pesticides / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D014197:Trees
D007202:Indicators and Reagents; D010575:Pesticides; D005278:Fenitrothion