Cdc2p controls the forkhead transcription factor Fkh2p by phosphorylation during sexual differentiation in fission yeast.
In most eukaryotes, cyclin-dependent kinases (Cdks) play a central role in control of cell-cycle progression. Cdks are inactivated from the end of mitosis to the start of the next cell cycle as well as during sexual differentiation. The forkhead-type transcription factor Fkh2p is required for the periodic expression of many genes and for efficient mating in the fission yeast Schizosaccharomyces pombe. However, the mechanism responsible for coordination of cell-cycle progression with sexual differentiation is still unknown. We now show that Fkh2p is phosphorylated by Cdc2p (Cdk1) and that phosphorylation of Fkh2p on T314 or S462 by this Cdk blocks mating in S. pombe by preventing the induction of ste11+ transcription, which is required for the onset of sexual development. We propose that functional interaction between Cdks and forkhead transcription factors may link the mitotic cell cycle and sexual differentiation.
Shimada
Midori
M
Department of Biochemistry and Cell Biology, Graduate School of Medicine, Nagoya City University, Mizuho-ku, Nagoya, Japan.
Yamada-Namikawa
Chisato
C
Murakami-Tonami
Yuko
Y
Yoshida
Takashi
T
Nakanishi
Makoto
M
Urano
Takeshi
T
Murakami
Hiroshi
H
D000595:Amino Acid Sequence; D016203:CDC2 Protein Kinase / Q000502:physiology*; D002453:Cell Cycle / Q000235:genetics; D003227:Conjugation, Genetic; D049770:Genes, Mating Type, Fungal / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism* / Q000502:physiology*; D012733:Sex Differentiation / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism*
C500536:Fkh2 protein, S pombe; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C071596:ste11 protein, S pombe; D016203:CDC2 Protein Kinase; C469447:cdc2 protein, S pombe