Human Y5 RNA specializes a Ro ribonucleoprotein for 5S ribosomal RNA quality control.
Humans express four distinct non-protein-coding Y RNAs (ncRNAs). To investigate Y RNA functional diversification, we exploited an RNA-based affinity purification method to isolate ribonucleoproteins (RNPs) assembled on individual human Y RNAs. Silver staining and mass spectrometry revealed that the Ro and La proteins assemble with all Y RNAs, while additional proteins associate with specific Y RNAs. Unexpectedly, Y5 RNA uniquely copurified ribosomal protein L5 and its binding partner 5S RNA. These findings reveal a contribution of Y5 to 5S surveillance and suggest that interactions between Ro-Y5 and L5-5S RNPs establish 5S RNA as a target of quality control.
Department of Molecular and Cell Biology, University of California at Berkely, Berkeley, California 94720, USA. jh2721@columbia.edu
J Robert
JR
Hogg
Kathleen
K
Collins
D000345:Affinity Labels; D001324:Autoantigens / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D004268:DNA-Binding Proteins; D014644:Genetic Variation; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism; D000072260:RNA Splicing Factors; D012341:RNA, Ribosomal, 5S / Q000235:genetics* / Q000378:metabolism*; D020733:RNA, Small Cytoplasmic / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism*; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012097:Repressor Proteins; D012261:Ribonucleoproteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D000099207:SS-A Antigen
D000345:Affinity Labels; D001324:Autoantigens; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D000072260:RNA Splicing Factors; D012341:RNA, Ribosomal, 5S; D020733:RNA, Small Cytoplasmic; D022661:RNA, Untranslated; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D012261:Ribonucleoproteins; D012269:Ribosomal Proteins; C110985:IFIT5 protein, human; C116006:RO60 protein, human; D000099207:SS-A Antigen; C410595:poly-U binding splicing factor 60KDa; C029742:ribosomal protein L5