Reactive oxygen species mediate sevoflurane- and desflurane-induced preconditioning in isolated human right atria in vitro.
BACKGROUND
We examined the role of reactive oxygen species (ROS) in sevoflurane- and desflurane-induced preconditioning on isolated human right atrial myocardium.


METHODS
We recorded isometric contraction of human right atrial trabeculae suspended in an oxygenated Tyrode's solution (34 degrees C, stimulation frequency 1 Hz). In all groups, a 30-min hypoxic period was followed by 60 min of reoxygenation. Ten minutes before hypoxia reoxygenation, muscles were exposed to 5 min of sevoflurane 2% or desflurane 6%. In separate groups, the sevoflurane 2% (Sevo + N-(2-mercaptopropionyl)-glycine [MPG]) or desflurane 6% (Des + MPG) was administered in the presence of 0.1 mM MPG, a ROS scavenger. The effect of 0.1 mM MPG alone was tested. Recovery of force after a 60-min reoxygenation period was compared between groups (mean +/- sd).


RESULTS
Preconditioning with sevoflurane 2% (85% +/- 4% of baseline) or desflurane 6% (86% +/- 7% of baseline) enhanced the recovery of the force of myocardial contraction after 60 min reoxygenation compared with the control group (53% +/- 11% of baseline, P < 0.001). This effect was abolished in the presence of MPG (56% +/- 12% of baseline for Sevo + MPG, 48% +/- 13% of baseline for Des + MPG). The effect of MPG alone on the recovery of force was not different from the control group (57% +/- 7% of baseline versus 53% +/- 11%; P = NS).


CONCLUSIONS
In vitro, sevoflurane and desflurane preconditioned human myocardium against hypoxia through a ROS-dependent mechanism.
Hanouz
Jean-Luc
JL
Department of Anesthesiology, CHU Caen, France. hanouz-jl@chu-caen.fr
Zhu
Lan
L
Lemoine
Sandrine
S
Durand
Charline
C
Lepage
Olivier
O
Massetti
Massimo
M
Khayat
André
A
Plaud
Benoît
B
Gérard
Jean-Louis
JL
D000328:Adult; D000368:Aged; D020517:Atrial Appendage / Q000187:drug effects / Q000502:physiology; D016280:Atrial Function, Right / Q000187:drug effects / Q000502:physiology*; D000077335:Desflurane; D006801:Humans; D066298:In Vitro Techniques; D019157:Ischemic Preconditioning, Myocardial / Q000379:methods*; D007530:Isoflurane / Q000031:analogs & derivatives* / Q000494:pharmacology; D008738:Methyl Ethers / Q000494:pharmacology*; D008875:Middle Aged; D017382:Reactive Oxygen Species / Q000378:metabolism*; D000077149:Sevoflurane
D008738:Methyl Ethers; D017382:Reactive Oxygen Species; D000077149:Sevoflurane; D000077335:Desflurane; D007530:Isoflurane