Characterization of the structural gene encoding nisin F, a new lantibiotic produced by a Lactococcus lactis subsp. lactis isolate from freshwater catfish (Clarias gariepinus).
Lactococcus lactis F10, isolated from freshwater catfish, produces a bacteriocin (BacF) active against Staphylococcus aureus, Staphylococcus carnosus, Lactobacillus curvatus, Lactobacillus plantarum, and Lactobacillus reuteri. The operon encoding BacF is located on a plasmid. Sequencing of the structural gene revealed no homology to other nisin genes. Nisin F is described.
Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland 7602, Stellenbosch, South Africa.
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D000595:Amino Acid Sequence; D000818:Animals; D001430:Bacteriocins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D002397:Catfishes / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009561:Nisin / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013210:Staphylococcus / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
D001430:Bacteriocins; D009561:Nisin