Inhibition of human coronavirus 229E infection in human epithelial lung cells (L132) by chloroquine: involvement of p38 MAPK and ERK.
The antiviral effects of chloroquine (CQ) on human coronavirus 229E (HCoV-229E) infection of human fetal lung cell line, L132 are reported. CQ significantly decreased the viral replication at concentrations lower than in clinical usage. We demonstrated that CQ affects the activation of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK). Furthermore, p38 MAPK inhibitor, SB203580, inhibits CPE induced by HCoV-229E infection and viral replication. Our findings suggest that CQ affects the activation of MAPKs, involved in the replication of HCoV-229E.
Kono
Masakazu
M
Department of Molecular Virology, Chiba University School of Medicine, 1-8-1 Inohana, Chiba 260-8670, Japan.
Tatsumi
Koichiro
K
Imai
Alberto M
AM
Saito
Kengo
K
Kuriyama
Takayuki
T
Shirasawa
Hiroshi
H
D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D002738:Chloroquine / Q000494:pharmacology*; D028941:Coronavirus 229E, Human / Q000187:drug effects*; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004847:Epithelial Cells / Q000821:virology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D008168:Lung / Q000166:cytology / Q000821:virology*; D011725:Pyridines / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014779:Virus Replication / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors*
D000998:Antiviral Agents; D007093:Imidazoles; D011725:Pyridines; D002738:Chloroquine; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580