DNA methylation patterns of the CDH1, RARB, and SFN genes in choroid plexus tumors.
Genetic and epigenetic alterations in choroid plexus tumors, a rare neuroepithelial neoplasm most frequently detected in children, are poorly characterized. Epigenetic silencing associated with aberrant CpG island methylation is one mechanism leading to the loss of tumor suppressor functions in cancer cells. Using methylation-specific polymerase chain reaction, the methylation patterns of the genes CDH1 (E-cadherin), RARB (retinoic acid receptor, beta), and SFN (stratifin; 14-3-3sigma) were retrospectively investigated in eight choroid plexus tumors (five papillomas, two atypical papillomas, and one carcinoma), as well as in two normal cortexes obtained after autopsy from male individuals aged 6 months and 64 years. Among the six pediatric tumors, the mean age at diagnosis was 1.8 years old (range, 0.2-6) and the two adult tumors were detected in a 66-year-old man and a 45-year-old woman. A high frequency of hypermethylation was detected in CDH1 and SFN genes in tumoral and normal cortex tissues. Tumor-specific RARB hypermethylation was observed in four papillomas. Further studies are required to evaluate the role of aberrant methylation in choroid plexus tumor progression.
Department of General Biology, Biological Sciences Center, State University of Londrina-UEL, Londrina, PR, Brazil.
Roberta
R
Losi-Guembarovski
Hellen
H
Kuasne
Alda L
AL
Guembarovski
Cláudia A
CA
Rainho
Ilce M S
IM
Cólus
D048948:14-3-3 Proteins; D000368:Aged; D015703:Antigens, CD; D014408:Biomarkers, Tumor / Q000235:genetics*; D015820:Cadherins / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D002831:Choroid Plexus / Q000378:metabolism; D016545:Choroid Plexus Neoplasms / Q000235:genetics*; D019175:DNA Methylation; D005092:Exonucleases / Q000235:genetics*; D005095:Exoribonucleases; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D010212:Papilloma / Q000235:genetics; D018168:Receptors, Retinoic Acid / Q000235:genetics*; D012189:Retrospective Studies
D048948:14-3-3 Proteins; D015703:Antigens, CD; D014408:Biomarkers, Tumor; C513384:CDH1 protein, human; D015820:Cadherins; D009363:Neoplasm Proteins; D018168:Receptors, Retinoic Acid; C082925:retinoic acid receptor beta; D005092:Exonucleases; D005095:Exoribonucleases; C413442:SFN protein, human