Chitotriosidase and YKL-40 in normal and pre-eclamptic pregnancies.
OBJECTIVE
To compare macrophage activation in normal and pre-eclamptic pregnancies by determining YKL-40 concentration and chitotriosidase activity in maternal and cord serum.


METHODS
In this prospective case-control study samples of maternal peripheral blood and umbilical venous blood were collected from 28 pre-eclamptic and 24 normotensive pregnant women and their newborns. YKL-40 concentration and chitotriosidase activity were determined by enzyme-linked immunoassay and fluorometry, respectively.


RESULTS
Chitotriosidase activity in maternal and cord serum and YKL-40 concentration in cord serum were significantly higher in pre-eclamptic pregnancies (P<0.001), but there was no significant difference in maternal serum levels of YKL-40 between the case and control groups (P>0.05). There was a significant positive correlation between diastolic blood pressure and (1) chitotriosidase activity in both maternal and cord serum and (2) cord serum concentration of YKL-40 (r=0.61, r=0.84, and r=0.58, respectively).


CONCLUSIONS
This study may be the first to demonstrate maternal and fetal macrophage activation in pre-eclampsia.
Madazli
Riza
R
Department of Obstetrics and Gynecology, Cerrahpasa Medical Faculty, Istanbul, Turkey. madazli@superonline.com
Kucur
Mine
M
Gezer
Altay
A
Isman
Ferruh
F
Bulut
Berk
B
D054392:Adipokines; D000328:Adult; D015415:Biomarkers / Q000097:blood; D001794:Blood Pressure / Q000502:physiology; D016022:Case-Control Studies; D000071451:Chitinase-3-Like Protein 1; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D006023:Glycoproteins / Q000097:blood*; D006596:Hexosaminidases / Q000097:blood*; D006801:Humans; D007231:Infant, Newborn; D037102:Lectins; D008262:Macrophage Activation / Q000502:physiology*; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy
D054392:Adipokines; D015415:Biomarkers; C084533:CHI3L1 protein, human; D000071451:Chitinase-3-Like Protein 1; D006023:Glycoproteins; D037102:Lectins; D006596:Hexosaminidases; C086400:chitotriosidase