Construction of a small-molecule-integrated semisynthetic split intein for in vivo protein ligation.
A new split intein-based protein ligation tool that is synthetically accessible and can be used for protein semisynthesis on the cell surface and potentially inside cells has been constructed.
Ando
Tomomi
T
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
Tsukiji
Shinya
S
Tanaka
Tsutomu
T
Nagamune
Teruyuki
T
D047668:Inteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019154:Protein Splicing; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D011506:Proteins