Biodegradation of methyl parathion by Acinetobacter radioresistens USTB-04.
Biodegradation of methyl parathion (MP), a widely used organophosphorus pesticide, was investigated using a newly isolated bacterium strain Acinetobacter radioresistens USTB-04. MP at an initial concentration of 1200 mg/L could be totally biodegraded by A. radioresistens USTB-04 as the sole carbon source less than 4 d in the presence of phosphate and urea as phosphorus and nitrogen sources, respectively. Biodegradation of MP was also achieved using cell-free extract of A. radioresistens USTB-04. MP at an initial concentration of 130 mg/L was completely biodegraded in 2 h in the presence of cell-free extract with a protein concentration of 148.0 mg/L, which was increased with the increase of pH from 5.0 to 8.0. Contrary to published reports, no intermediate or final degradation metabolites of MP could be observed. Thus we suggest that the cleavage of C-C bond on the benzene ring other than P-O bond may be the biodegradation pathway of MP by A. radioresistens USTB-04.
Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, Beijing 100083, China.
Fang-yao
FY
Liu
Ming-zhang
MZ
Hong
Dan-mei
DM
Liu
Ya-wen
YW
Li
Pei-shun
PS
Shou
Hai
H
Yan
Guo-qing
GQ
Shi
D000150:Acinetobacter / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D008743:Methyl Parathion / Q000378:metabolism*; D011522:Protons; D013696:Temperature
D011522:Protons; D008743:Methyl Parathion