Proteomic analysis to identify the role of LuxS/AI-2 mediated protein expression in Escherichia coli O157:H7.
Microorganisms employ autoinducer molecules to modulate various bacterial processes including virulence expression, biofilm development, and bioluminescence. The universal autoinducer molecule AI-2 is hypothesized to mediate cell signaling in Escherichia coli O157:H7. We investigated the role of AI-2 on the E. coli O157:H7 cellular proteins using a two-dimensional (2D) gel electrophoresis-based proteomic approach. The protein expression patterns between two experimental comparisons were studied namely, 1) a wild type E. coli O157:H7 and its isogenic luxS mutant, and 2) the luxS mutant and the luxS mutant supplemented with AI-2 molecules. Eleven proteins were differentially expressed between the wild type and the luxS mutant strain, whereas 18 proteins were differentially expressed in the luxS mutant strain when supplemented with AI-2. The tryptophan repressor binding protein (WrbA), phosphoglycerate mutase (GpmA), and a putative protein YbbN were found to be differentially expressed under both experimental comparisons. The FliC protein which is involved in flagellar synthesis and motility was up-regulated in the wild type strain but was not influenced by the addition of synthetic AI-2 molecules to the luxS mutant suggesting the involvement of signaling molecules other than AI-2 on flagellar synthesis and motility.
Soni
Kamlesh
K
Food Safety & Environmental Microbiology Program, Department of Poultry Science, Texas A&M University, College Station, Texas, 77843, USA.
Jesudhasan
Palmy
P
Cepeda
Martha
M
Williams
Brad
B
Hume
Michael
M
Russell
William K
WK
Jayaraman
Arul
A
Pillai
Suresh D
SD
D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology*; D013437:Carbon-Sulfur Lyases / Q000235:genetics* / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D019453:Escherichia coli O157 / Q000235:genetics / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000502:physiology*; D005407:Flagella / Q000235:genetics / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation; D040901:Proteomics; D015398:Signal Transduction; D015854:Up-Regulation; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria