Inhibition of low-density lipoprotein oxidation and oxidative burst in polymorphonuclear neutrophils by caffeic acid and hispidin derivatives isolated from sword brake fern (Pteris ensiformis Burm.).
Several antioxidant compounds have been previously identified from sword brake fern (Pteris ensiformis Burm.) by DPPH bleaching and Trolox equivalent antioxidant capacity (TEAC) analyses. Among the isolates, 7-O-caffeoylhydroxymaltol 3-O-beta-D-glucopyranoside and hispidin 4-O-beta- D-glucopyranoside [6-(3,4-dihydroxystyryl)-4-O-beta-D-glucopyranoside-2-pyrone] were two new compounds. The aim of this study is to elucidate the possible effect of the aqueous extract of sword brake fern (SBF) and these two compounds in preventing atherosclerosis. The results demonstrated that SBF and these two compounds strongly inhibited Cu2+-mediated low-density lipoprotein (LDL) oxidation measured by thiobarbituric acid-reactive substances assay (TBARS), conjugated diene production, and relative electrophoretic mobility. The commercial antioxidant dl-alpha-tocopherol showed lower antioxidant activity than these two compounds at the same molecular concentration. SBF and these two compounds also suppressed N-formylmethionyl-leucylphenylalanine (fMLP)-stimulated reactive oxygen species (ROS) production in human polymorphonuclear neutrophils (PMN). These findings indicate that sword brake fern may prevent atherosclerosis via inhibition of both LDL oxidation and ROS production.
Department of Biotechnology, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan.
Hsiu-An
HA
Wei
Tzi-Wei
TW
Lian
Yi-Chen
YC
Tu
Jing-Ting
JT
Hong
Mei-Chun
MC
Kou
Ming-Jiuan
MJ
Wu
D002109:Caffeic Acids / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000097:blood; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D032495:Pteris / Q000737:chemistry*; D011753:Pyrones / Q000302:isolation & purification / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000097:blood; D016897:Respiratory Burst / Q000187:drug effects*
D002109:Caffeic Acids; D008077:Lipoproteins, LDL; D011753:Pyrones; D017382:Reactive Oxygen Species; C007946:hispidin; C040048:caffeic acid