Modified expression of cytoplasmic isocitrate dehydrogenase electrophoretic isoforms in seminal plasma of men with sertoli-cell-only syndrome and seminoma.
Two isoforms of human cytoplasmic isocitrate dehydrogenase (IDPc) of close molecular weights and different isoelectric points were identified in human seminal plasma (SP) by two-dimensional gel electrophoresis (2-DE) followed by mass spectrometry (MS). These two isoforms were detected in the normospermic men SP and their expressions were markedly altered in patients with testicular seminoma, the most frequent testicular germ cell cancer (TGCC): increase of the more acidic spot and decrease of the more basic one. Since oligospermia has been considered as a high risk pathological condition for developing a testicular cancer, the two IDPc isoforms were analyzed in SP of a group of secretory azoospermic patients. In this group the two spots displayed similar variations of expression to those observed in testicular seminoma. These results propose IDPc as a promising SP biomarker of testicular seminoma. Whether IDPc alteration in secretory azoospermia is predictive of testicular seminoma remains to be elucidated.
INSERM U.670, UFR de Médecine, Nice Cedex 2, France.
Mireille
M
Starita-Geribaldi
Michel
M
Samson
Jean-Marie
JM
Guigonis
Georges
G
Pointis
Patrick
P
Fenichel
D003593:Cytoplasm / Q000201:enzymology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D008297:Male; D012661:Semen / Q000201:enzymology*; D018239:Seminoma / Q000201:enzymology*; D054331:Sertoli Cell-Only Syndrome / Q000201:enzymology*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013736:Testicular Neoplasms / Q000201:enzymology*
D007521:Isocitrate Dehydrogenase