Tailor-made fructooligosaccharides by a combination of substrate and genetic engineering.
The combination of sucrose analogues as novel substrates (substrate engineering) and highly active recombinant beta-fructofuranosidase from A. niger (genetic engineering) provides a new powerful tool for the efficient preparative synthesis of tailor-made saccharides of the important 1-kestose and 1-nystose type headed with different monosaccharides of interest. These novel compounds have been isolated. They did not display toxic effects or suppress cell growth in initial studies, making these new compounds potential candidates for prebiotics.
Zuccaro
Alga
A
Department of Microbiology, Technical University of Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany.
Götze
Sven
S
Kneip
Susanne
S
Dersch
Petra
P
Seibel
Jürgen
J
D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology; D005818:Genetic Engineering; D006801:Humans; D007700:Kinetics; D009005:Monosaccharides / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology; D009844:Oligosaccharides / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D014312:Trisaccharides / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology; D043324:beta-Fructofuranosidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D009005:Monosaccharides; D009844:Oligosaccharides; D011994:Recombinant Proteins; D014312:Trisaccharides; C116580:fructooligosaccharide; C072433:1-kestose; D043324:beta-Fructofuranosidase