Detection and kinetics of mucosal pathogenic bacteria binding with polysaccharides.
The detection and kinetics of mucosal pathogenic bacteria binding on polysaccharide ligands were studied using a surface plasmon resonance biosensor. The kinetic model applied curve-fitting to the experimental surface plasmon resonance sensorgrams to evaluate the binding interactions. The kinetic parameters for the mucosal pathogenic bacteria (Pseudomonas aeruginosa, Pseudomonasfluorescens, Serratia marcescens) with the alginate ligand were determined from a kinetic model. In addition, the binding interactions of the mucosal pathogenic bacteria with polysaccharide binding pairs (Pseudomonas aeruginosa/alginate, Streptococcus pneumoniae/pneumococcal polysaccharide, Staphylococcus aureus/pectin) were also compared with their kinetic parameters. The rate constants of association for Pseudomonas aeruginosa with the alginate ligand were higher than those for Pseudomonas fluorescens. Serratia marcescens had no detectable interaction with the alginate ligand. The adhesion affinity of Pseudomonas aeruginosa with alginate was higher than that for the other binding pairs. The binding affinities of the pathogenic bacteria with their own polysaccharide were higher than that of Staphylococcus aureus with pectin. Measuring the contact angle was found to be a feasible method for detecting binding interactions between analytes and ligands.
Center for Functional Nano Fine Chemicals (Post BK21 Program), Chonnam National University, Gwangiu 500-757, Korea.
Kyong-Hwan
KH
Chung
Jung-Soon
JS
Park
Hyun-Soo
HS
Hwang
Jin-Chul
JC
Kim
Ki-Young
KY
Lee
D000464:Alginates / Q000737:chemistry / Q000378:metabolism; D001419:Bacteria / Q000472:pathogenicity*; D001422:Bacterial Adhesion / Q000502:physiology*; D003198:Computer Simulation; D020723:Glucuronic Acid / Q000737:chemistry / Q000378:metabolism; D006603:Hexuronic Acids / Q000737:chemistry / Q000378:metabolism; D007413:Intestinal Mucosa / Q000502:physiology*; D007700:Kinetics; D008024:Ligands; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D011551:Pseudomonas fluorescens / Q000502:physiology; D020349:Surface Plasmon Resonance / Q000379:methods; D014783:Viscosity
D000464:Alginates; D006603:Hexuronic Acids; D008024:Ligands; D011134:Polysaccharides; D020723:Glucuronic Acid