Biological control of strawberry gray mold caused by Botrytis cinerea using Bacillus licheniformis N1 formulation.
Bacillus licheniformis N1 is a biological control agent to control gray mold diseases caused by Botrytis cinerea. Various formulations of B. licheniformis N1 were generated and evaluated for the activity to control strawberry gray mold. The wettable powder type formulation N1E was selected in pot experiments with remarkable disease control activity on both strawberry leaves and flowers. The N1E formulation contained 400 g of corn starch, 50 ml of olive oil, and 50 g of sucrose per a liter of bacterial fermentation culture. Optimum dilution of N1E to appropriately control the strawberry gray mold appeared to be 100-fold dilution in plastic house artificial infection experiments. The significant reduction of symptom development in the senescent leaves was apparent by the treatment of N1E at 100-fold dilution when N1E was applied before Bo. cinerea inoculation, but not after the inoculation. Both artificial infection experiments in a plastic house and natural infection experiments in the farm plastic house under production conditions revealed that the disease severity of gray mold on strawberry leaves and flowers was significantly reduced by N1E treatment. The disease control value of N1E on strawberry leaves was 81% under production conditions, as compared with the 61.5% conferred by a chemical fungicide, iprodione. This study suggests that our previously generated formulation of B. licheniformis N1 will be effective to control strawberry gray mold by its preventive activity.
Kim
Ju Hyun
JH
Division of Applied Biology, College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea.
Lee
Soo Hee
SH
Kim
Choul Sung
CS
Lim
Eun Kyung
EK
Choi
Ki Hyuck
KH
Kong
Hyun Gi
HG
Kim
Dae Wook
DW
Lee
Seon-Woo
SW
Moon
Byung Ju
BJ
D001407:Bacillus / Q000254:growth & development*; D020171:Botrytis / Q000254:growth & development*; D035264:Flowers / Q000382:microbiology; D031985:Fragaria / Q000382:microbiology; D005638:Fruit / Q000382:microbiology; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology