[An study on screening the gene clusters associated with pulmonary embolism-deep venous thrombosis by oligo microarray].
OBJECTIVE
To find out the gene clusters associated with pulmonary embolism-deep venous thrombosis (PE-DVT) and elucidate the molecular genetic mechanism of PE.


METHODS
Peripheral venous blood samples were collected from 9 PE patients and 33 normal controls. The total RNA was extracted and purified. Nine PE cRNA probes labeled with cyanine 3 were constructed, and one standard cRNA probe labeled with cyanine 5 was synthesized based on the total RNA mixture of the controls. Hybridization with Agilent Whole Human Genome Oligo Microarray was performed. Eleven of the genes screened were randomly selected to be amplified by fluorescence quantitative PCR (FQ-PCR).


RESULTS
434 differential expression genes were screened from the 9 microarrays, including 36 gene transcripts. Associated with blood coagulation, 20 with immune or inflammatory response, 29 with metabolism, 26 with cell differentiation and apoptosis, 25 with cell growth/maintenance, 22 with cell-cell signaling or signal transduction, 14 with cytoskeleton or motility, 15 with ion channel or ion transport, 14 with transcription, and 6 with DNA/RNA binding. These 11 of these genes were randomly selected to be amplified by FQ-PCR. The differential expression of the 11 selected genes was consistent with the microarray.


CONCLUSIONS
The differential expression of a lot of genes in the body may play a role in the process of initiation and development of PE-DVT.
Department of Respiratory Medicine, Shanghai Pneumology Hospital, Shanghai 200433, China.
Ke-bin
KB
Cheng
Le-min
LM
Wang
Heng-jun
HJ
Gao
Ying
Y
Hu
D000328:Adult; D000368:Aged; D002232:Carbocyanines / Q000737:chemistry; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D011655:Pulmonary Embolism / Q000235:genetics*; D020246:Venous Thrombosis / Q000235:genetics*
D002232:Carbocyanines; C085321:cyanine dye 5