The DNA-repair Ku70 protein is located in the nucleus and tail of elongating spermatids in grasshoppers.
Fluorescence immunostaining for the phosphorylated H2AX histone (gammaH2AX) in the grasshopper Eyprepocnemis plorans has shown abundance of gammaH2AX in the nuclei of round and elongating spermatids, suggesting that DNA double-strand breaks (DSBs) occur regularly during spermiogenesis. Immunofluorescence patterns for Ku70, a DNA-repair protein participating in the non-homologous end-joining (NHEJ) pathway, showed that this protein is present in round and elongating spermatids, implying that the NHEJ DNA-repair pathway operates during chromatin compaction in spermiogenesis. In addition, during the final stages of spermiogenesis, the Ku70 protein concentrates on the region forming the sperm tail. Since Ku70 was also abundant in spermatid tails, it is reasonable to assume that Ku70 might play a novel function in sperm-tail formation. The analysis of Ku70 immunofluorescence patterns in 13 other grasshopper species also showed the presence of this protein in the nucleus and tail of elongating spermatids, indicating that this is a general characteristic in grasshoppers.
Departamento de Genética, Universidad de Granada, 18071 Granada, Spain.
Josefa
J
Cabrero
Rogelio J
RJ
Palomino-Morales
Juan Pedro M
JP
Camacho
D000818:Animals; D034961:Antigens, Nuclear / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000235:genetics*; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006110:Grasshoppers / Q000235:genetics* / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D015151:Immunoblotting; D000072200:Ku Autoantigen; D008297:Male; D013087:Spermatids / Q000502:physiology*; D013091:Spermatogenesis
D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D006657:Histones; D000072200:Ku Autoantigen