[Effects of low dosage pyrene pollution on biochemical characters of earthworm (Eisenia fetida) in soil].
By the method of artificial soil pollution, an exposure experiment with different concentrations of pyrene (0, 60, 120, 240, 480, 960 microg x kg(-1)) was conducted to determine the cytochrome P450 and MDA contents and the glutathione-S-transferase (GST), superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities in earthworm gut after exposure for 1, 3, 7 and 14 days. The results indicated that within the range of test pyrene concentrations, all the biochemical indices tested differed in their sensitivity to pyrene toxicity, among which, P450 content and GST and SOD activities were most sensitive, followed by POD and CAT activities, while MDA content did not show any obvious response. Exposure duration had stronger effects than exposure dosage. In diagnosing the ecotoxicity of soil pollutant, it could be necessary to use a combined multi-time and multi-index diagnostic method to enhance the sensitivity and effectiveness of the indices adopted.
Zhang
Wei
W
Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China. Zhangweilm04@163.com
Song
Yu-Fang
YF
Sun
Tie-Heng
TH
Liu
Miao
M
Ackland
M L
ML
Galina
Belogolova
B
D000818:Animals; D002374:Catalase / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004064:Digestive System / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005982:Glutathione Transferase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009835:Oligochaeta / Q000187:drug effects / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D011721:Pyrenes / Q000633:toxicity*; D012989:Soil Pollutants / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
D011721:Pyrenes; D012989:Soil Pollutants; D008315:Malondialdehyde; D003577:Cytochrome P-450 Enzyme System; C030984:pyrene; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase