[The changes and significance of microsatellite loss of heterozygosity in laryngeal premalignant lesions and squamous cell carcinoma].
OBJECTIVE
To evaluate the character and significance of the loss of heterozygosity (LOH) on chromosomes 3P, 9P and 17P in laryngeal premalignant and malignant lesions.


METHODS
Allelic deletions analysis was performed with 6 polymorphic markers (D3S1234, D9S171, D9S1748, D9S162, INFA and p53) using polymerase chain reaction-single sequence length polymorphism-silver staining in 49 laryngeal premalignant and malignant lesions.


RESULTS
Allelic loss was seen in 3.70% of the six markers in patients with simple hyperplasias (HP), 10.81% with low grade dysplasia (LGD), 26.03% with high grade dysplasia (HGD) and 38.67% with laryngeal squamous cell carcinoma (LSCC). Significant differences was found between allelic loss and clinical pathological grades (X2 = 17.686, P < 0.01). The rate of LOH increased remarkably with the lesions progressed from HP to LSCC. D9S171showed the highest incidence of LOH (35.00%) on the six markers.


CONCLUSIONS
Microsatellite DNA LOHs occur in early stage of laryngeal carcinogenesis. Microsatellite markers may be useful in the early diagnosis for laryngeal premalignant lesions.
Department of Otorhinolaryngology-Head and Neck Surgery, the Affiliated Province Hospital, Anhui Medical University, Hefei, 230001, China.
Tao
T
Guo
Jingwu
J
Sun
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D009367:Neoplasm Staging; D011230:Precancerous Conditions / Q000235:genetics* / Q000473:pathology