Rapid and reliable peptide de novo sequencing facilitated by microfluidic chip-based Edman degradation.
This paper expands the application of the newly developed highly sensitive microfluidic chip-based Edman degradation system. Comparison between the MS/MS spectra of a native peptide and its N-terminus truncated counterpart after carrying out one cycle of Edman degradation in a microfluidic chip can not only provide N-terminal residue information, but also facilitate the identification of different series of fragment ions. Manual peptide sequencing is more feasible and rapid using this method as demonstrated with three peptide examples including one neuropeptide. Furthermore, two cycles of Edman degradation allow the determination of the exact value of b 2 ion of the intact peptide, which can serve as an internal calibrant to increase the mass accuracy of the MS/MS spectrum.
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310027, China.
Wenzhang
W
Chen
Xuefeng
X
Yin
Yan
Y
Yin
D046210:Microfluidic Analytical Techniques; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D040081:Protein Array Analysis; D020539:Sequence Analysis, Protein; D021241:Spectrometry, Mass, Electrospray Ionization
D009479:Neuropeptides; D010455:Peptides