Brandisianins A-F, isoflavonoids isolated from Millettia brandisiana in a screening program for death-receptor expression enhancement activity.
In a screening study for natural products with tumor-selective apoptosis-inducing properties, six new isoflavonoids (1- 6), named brandisianins A-F, respectively, have been isolated from a MeOH extract of the dried leaves of Millettia brandisiana, together with five known compounds. The structures of the new compounds were elucidated by spectroscopic data interpretation. Among these compounds, brandisianin D (4) exhibited death-receptor 5 expression enhancement activity in a luciferase assay based in DLD-1/ SacI cells. The results suggest that brandisianin D (4) might overcome TRAIL-resistance by an increase in DR5 expression.
Kikuchi
Hiroyuki
H
Graduate School of Pharmaceutical Sciences, Chiba University, Chiba, Japan.
Ohtsuki
Takashi
T
Koyano
Takashi
T
Kowithayakorn
Thaworn
T
Sakai
Toshiyuki
T
Ishibashi
Masami
M
D006801:Humans; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008156:Luciferases / Q000378:metabolism; D033641:Millettia / Q000737:chemistry*; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000032:analysis / Q000378:metabolism*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000187:drug effects; D013785:Thailand
D007529:Isoflavones; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D053221:TNF-Related Apoptosis-Inducing Ligand; D008156:Luciferases