[Study on the characteristics of tissue expression of hormone sensitive lipase and triacylglycerol hydrolase in pigs].
The specific expression of TGH and HSL genes in different tissues of Bamei pig was investigated by RT-PCR and Western blot in this study. The result of RT-PCR showed that the expression of HSL could be detected in all these seven tissues examined, and which was higher expressed in fat, lower in heart, liver, lung, spleen and kidney. Expression of TGH gene could also be detected in seven tissues, and higher in liver and fat, lower in heart and kidney and lowest in spleen and lung. The result of Western blot showed that, HSL gene was highest expressed in epiploica fat and subcutaneous fat, higher in other tissues, but couldn' t be detected in kidney. Expression of TGH was detected in epiploica fat, subcutaneous fat, liver, lung and spleen, and highest in fat and liver, but it hadn't be found in heart and kidney. These results suggested that both HSL and TGH could be regulated by post-transcriptional, and their function was involved in different tissues.
Wang
Qi
Q
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Zhang
Li-Hong
LH
Zhang
Li-Jie
LJ
Yang
Gong-She
GS
D000818:Animals; D005786:Gene Expression Regulation; D008049:Lipase / Q000235:genetics* / Q000378:metabolism; D008297:Male; D002787:Sterol Esterase / Q000235:genetics* / Q000378:metabolism; D013552:Swine; D014018:Tissue Distribution
D002787:Sterol Esterase; D008049:Lipase