High performance thin layer chromatographic analysis of neutral lipids in the urine of BALB/c mice infected with Echinostoma caproni.
Silica gel high performance thin layer chromatography-densitometry was used to determine the neutral lipid profile in the urine of BALB/c mice experimentally infected with adults of Echinostoma caproni. An approximate 30 worm burden per host induced certain changes in the neutral lipid profile of the mice at 2 to 10 weeks post-infection (PI) relative to the uninfected controls. Infection caused a significant increase (Student's t test, P < 0.05) in the methyl oleate fraction at 6 and 7 weeks PI relative to the uninfected controls. Neutral lipid profiles of urine may serve as an indicator of experimental infection of echinostomes in mice.
Department of Chemistry, Lafayette College, Easton, PA, USA.
James D
JD
Vasta
Bernard
B
Fried
Joseph
J
Sherma
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004449:Echinostoma / Q000472:pathogenicity*; D004451:Echinostomiasis / Q000469:parasitology* / Q000652:urine; D005260:Female; D008055:Lipids / Q000032:analysis* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D009829:Oleic Acids / Q000032:analysis; D014556:Urine / Q000737:chemistry*
D008055:Lipids; D009829:Oleic Acids; C005576:methyl oleate