Antifungal cyclopeptolide from fungal saprophytic antagonist Ulocladium atrum.
The saprophytic fungus Ulocladium atrum Preuss is a promising biological control agent for Botrytis cinerea in greenhouse- and field-grown crops. However, despite its known potent antifungal activity, no antifungal substance has yet been reported. In an effort to characterize the antifungal substance from U atrum, we isolated an antibiotic peptide. Based on extensive spectroscopic analyses, its structure was established as a cyclopeptolide with a high portion of N-methylated amino acids, and its 1H and 13C chemical shifts were completely assigned based on extensive 1D and 2D NMR experiments. Compound 1 exhibited potent antifungal activity against the plant pathogenic fungus Botrytis cinerea and moderate activity against Alternaria alternate and Magnaporthe grisea.
Yun
Bong-Sik
BS
Functional Metabolomics Research Center, KRIBB, Daejeon 305-333, Korea.
Kwon
Eun Mi
EM
Kim
Jin-Cheol
JC
Yu
Seung Hun
SH
D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification*; D001203:Ascomycota / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000302:isolation & purification*
D000935:Antifungal Agents; D010456:Peptides, Cyclic; C088262:cyclopeptolide 1