Functional characterisation of the Schizosaccharomyces pombe homologue of the leukaemia-associated translocation breakpoint binding protein translin and its binding partner, TRAX.
Translin is a conserved protein which associates with the breakpoint junctions of chromosomal translocations linked with the development of some human cancers. It binds to both DNA and RNA and has been implicated in mRNA metabolism and regulation of genome stability. It has a binding partner, translin-associated protein X (TRAX), levels of which are regulated by the translin protein in higher eukaryotes. In this study we find that this regulatory function is conserved in the lower eukaryotes, suggesting that translin and TRAX have important functions which provide a selective advantage to both unicellular and multi-cellular eukaryotes, indicating that this function may not be tissue-specific in nature. However, to date, the biological importance of translin and TRAX remains unclear. Here we systematically investigate proposals that suggest translin and TRAX play roles in controlling mitotic cell proliferation, DNA damage responses, genome stability, meiotic/mitotic recombination and stability of GT-rich repeat sequences. We find no evidence for translin and/or TRAX primary function in these pathways, indicating that the conserved biochemical function of translin is not implicated in primary pathways for regulating genome stability and/or segregation.
North West Cancer Research Fund Institute, University of Wales Bangor, Bangor, Gwynedd, LL57 2UW, United Kingdom.
Alessa
A
Jaendling
Soshila
S
Ramayah
David W
DW
Pryce
Ramsay J
RJ
McFarlane
D001483:Base Sequence; D002352:Carrier Proteins / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D004271:DNA, Fungal / Q000378:metabolism; D005057:Eukaryotic Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008540:Meiosis / Q000187:drug effects; D053842:Microsatellite Instability / Q000187:drug effects; D018895:Microsatellite Repeats; D008938:Mitosis / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity; D050505:Mutant Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D016601:RNA-Binding Proteins / Q000378:metabolism*; D011995:Recombination, Genetic / Q000187:drug effects; D012492:Salts / Q000494:pharmacology; D012568:Schizosaccharomyces / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013827:Thiabendazole / Q000494:pharmacology
D002352:Carrier Proteins; D004271:DNA, Fungal; D009153:Mutagens; D050505:Mutant Proteins; D016601:RNA-Binding Proteins; D012492:Salts; D029702:Schizosaccharomyces pombe Proteins; C502801:TRAX protein, S pombe; C502800:tsn1 protein, S pombe; D046988:Proteasome Endopeptidase Complex; D013827:Thiabendazole