Enzymatic activities in brains of diabetic rats treated with vanadyl sulphate and sodium tungstate.
The hypothesis of the present study was that diabetes mellitus might affect brain metabolism. Streptozotocin (STZ)-induced diabetic rats, treated with vanadyl sulphate (V) and sodium tungstate (T) were employed to observe the aspartate aminotransferase (AST), alanine aminotransferase (ALT) and creatine kinase (CK) activities in brain homogenates. Significant increases in AST, ALT and CK activities were found in diabetic brain homogenates against controls, suggesting increments of transamination in brain and/or increases in cell membrane permeability to these enzymes. The increase in brain CK possibly expresses alterations in energy production. The decrease in CK activity caused by V and T treatment in diabetic rats suggests that both agents tend to normalize energy consumption. It is also possible that V and T-induced hypoglycemic effects cause metabolic alterations in brain.
Lemberg
A
A
Cátedra de Fisiopatología, Facultad de Farmacia y Bioquímica, UBA, Junín 956, 6th floor, 1113 Buenos Aires, República Argentina.
Fernández
M A
MA
Ouviña
G
G
Rodríguez
R R
RR
Peredo
H A
HA
Susemihl
C
C
Villarreal
I
I
Filinger
E J
EJ
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D003402:Creatine Kinase / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism*; D004734:Energy Metabolism / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017973:Tungsten Compounds / Q000494:pharmacology*; D017968:Vanadium Compounds / Q000494:pharmacology*
D007004:Hypoglycemic Agents; D017973:Tungsten Compounds; D017968:Vanadium Compounds; C025399:sodium tungstate(VI); C034028:vanadyl sulfate; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003402:Creatine Kinase