The expression of endothelin receptor B in melanoma cells A375 and Sk-mel-1 and the proliferative effects of endothelin 3 on A375 cells.
In order to investigate the expression of endothelin receptor B (ETR-B) in human malignant melanoma (MM) cells A375 and SK-mel-1 and the proliferative effects of endothelin 3 (ET3) on A375 cells, RT-PCR was applied to detect the expression of ETR-B gene in human MM cells A375 and SK-mel-1. MTT method was used to evaluate the growth enhancing effects of ET3 on A375 cell line in vitro. The results showed that ETR-B gene was expressed in both MM A375 and SK-mel-1 cells. ET3 had stronger ability to enhance the proliferation of A375 cells in vitro in a concentration-dependent manner. It was suggested that ET3/ETR-B might play an important proliferative role in MM.
Department of Dermatology, Union Hospital Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. linnengxing@hotmail.com
Nengxing
N
Lin
Changzheng
C
Huang
Jin
J
Tian
Juan
J
Tao
Jin
J
Zhang
Lingyun
L
Yang
Yan
Y
Li
Yeqiang
Y
Liu
Siyuan
S
Chen
Guanxin
G
Shen
Jiawen
J
Li
Chunsen
C
Wang
Yating
Y
Tu
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D019334:Endothelin-3 / Q000494:pharmacology*; D006801:Humans; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology*; D044023:Receptor, Endothelin B / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D019334:Endothelin-3; D044023:Receptor, Endothelin B