Biosynthesis of bile acids in a variety of marine bacterial taxa.
Several marine bacterial strains, which were isolated from seawater off the island Dokdo, Korea, were screened to find new bioactive compounds such as antibiotics. Among them, Donghaeana dokdonensis strain DSW-6 was found to produce antibacterial agents, and the agents were then purified and analyzed by LC-MS/MS and 1D- and 2D-NMR spectrometries. The bioactive compounds were successfully identified as cholic acid and glycine-conjugated glycocholic acid, the 7alpha-dehydroxylated derivatives (deoxycholic acid and glycodeoxycholic acid) of which were also detected in relatively small amounts. Other masine isolates, taxonomically different from DSW-6, were also able to produce the compounds in a quite different production ratio from DSW-6. As far as we are aware of, these bile acids are produced by specific members of the genus Streptomyces and Myroides, and thought to be general secondary metabolites produced by a variety of bacterial taxa that are widely distributed in the sea.
Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Korea.
Dockyu
D
Kim
Jong Suk
JS
Lee
Jiyoung
J
Kim
So-Jung
SJ
Kang
Jung-Hoon
JH
Yoon
Won Gon
WG
Kim
Choong Hwan
CH
Lee
D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*; D001709:Biotechnology; D019826:Cholic Acid / Q000096:biosynthesis / Q000737:chemistry; D002793:Cholic Acids / Q000096:biosynthesis* / Q000737:chemistry; D041964:Cytophagaceae / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D003840:Deoxycholic Acid / Q000096:biosynthesis / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D006000:Glycocholic Acid / Q000096:biosynthesis / Q000737:chemistry; D006002:Glycodeoxycholic Acid / Q000096:biosynthesis / Q000737:chemistry; D007723:Korea; D012240:Rhodococcus / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D012623:Seawater / Q000382:microbiology*
D002793:Cholic Acids; D003840:Deoxycholic Acid; D006002:Glycodeoxycholic Acid; D019826:Cholic Acid; D006000:Glycocholic Acid