[Correlation between the function of monocytes/macrophages and disease progression in people living with HIV/AIDS in several provinces in China].
OBJECTIVE
To investigate the functional states of peripheral blood monocytes/macrophages and their relation with the disease progression in people living with HIV/AIDS (PWHA).


METHODS
Peripheral blood samples were collected from 57 PWHA who didn't receive antivirus treatment before and 32 healthy controls in Jilin, Liaoning, and Henan provinces. Flow cytometry was used to detect the expression of the early activation molecule CD69 in the CD14(+) cells. The plasma HIV RNA level was determined using real-time PCR.


RESULTS
The CD69 expression rate in the CD14(+) T cells of the PWHA group was 27% +/- 4%, significantly higher than that of the controls (P < 0.01). The CD69 expression rate of the AIDS group was 39% +/- 3%, significantly higher than those of the HIV group and long-term nonprogressors (LTNP) group (26% +/- 3% and 20% +/- 4% respectively, both P < 0.05), that of the HIV groups being significantly higher than that of the LTNP group too (P < 0.05). The side scatter value of the CD14(+) T cells of the PWHA group was 76 +/- 16, significantly higher than that of the control group (50 +/- 12, P < 0.05). The monocyte/macrophage CD69 expression rate was significantly negatively correlated with the absolute value of CD4(+) T cells (r = -0.872, P < 0.01), and not correlated with the value of CD8(+) T cells (P > 0.05). The monocyte/macrophage CD69 expression rate was significantly positively correlated with the HIV-1 RNA viral load (r = 0.697, P < 0.01).


CONCLUSIONS
The activation and the phagocytosis function of the monocytes/macrophages of the PWHA in China are higher than those of the healthy control. They are positively correlated with the disease progression.
Key Laboratory of Immunology of AIDS, Ministry of Health, First Hospital, China Medical University, Shenyang 110001, China.
Li-ping
LP
Zhou
Hong
H
Shang
Zi-ning
ZN
Zhang
Ya-nan
YN
Wang
Ge-fei
GF
Li
Wan-ying
WY
Shi
Hai-bo
HB
Ding
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood* / Q000276:immunology / Q000821:virology; D000328:Adult; D015703:Antigens, CD / Q000097:blood; D000945:Antigens, Differentiation, T-Lymphocyte / Q000097:blood; D015704:CD4 Antigens / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D002681:China; D005260:Female; D005434:Flow Cytometry; D015658:HIV Infections / Q000097:blood* / Q000276:immunology / Q000821:virology; D006801:Humans; D037181:Lectins, C-Type; D018950:Lipopolysaccharide Receptors / Q000097:blood; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D012367:RNA, Viral / Q000097:blood; D019562:Viral Load; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D037181:Lectins, C-Type; D018950:Lipopolysaccharide Receptors; D012367:RNA, Viral; D000099192:CD69 Antigens