Lack of association of single nucleotide polymorphism in LRCH1 with knee osteoarthritis susceptibility.
A genetic association of knee osteoarthritis (OA) and a C/T transition single nucleotide polymorphism (SNP) (rs912428) located in intron 1 of the LRCH1 gene has recently been reported in European Caucasians; however, the results are inconsistent. Our objective was to evaluate the association in different knee OA populations. Three case-control association studies were conducted in Han Chinese, Japanese, and Greek Caucasian populations. The LRCH1 SNP was genotyped in patients who had primary symptomatic knee OA with radiographic confirmation and in matched controls, and the association was examined. We performed a meta-analysis for the studies together with results of two previous papers using the DerSimonian-Laird procedure and calculated the power of the pooled studies by the software R. A total of 1,145 OA patients and 1,266 controls were genotyped. No significant difference was detected in genotype or allele frequencies between knee OA and control groups in the three populations (all P > 0.05). Association was not observed even after stratification by gender and Kellgren/Lawrence (K/L) scores. Meta-analysis also supported the lack of association between LRCH1 and knee OA. The strong heterogeneity between original and replication studies was detected in Caucasian populations. However, a tendency for the increase of TT genotype was observed in the European populations (OR = 1.46, P = 0.06). The powers for European and Asian replication studies were less than 0.8. Our results suggest that there is no association between LRCH1 and knee OA. However, lack of association should be concluded by further replication studies.
The Center of Diagnosis and Treatment for Joint Disease, Drum Tower Hospital Affiliated to Medical School of Nanjing University, Nanjing, 210008, Jiangsu, China. qingj@nju.edu.cn.
Qing
Q
Jiang
The Center of Diagnosis and Treatment for Joint Disease, Drum Tower Hospital Affiliated to Medical School of Nanjing University, Nanjing, 210008, Jiangsu, China.
Dongquan
D
Shi
Laboratory for Bone and Joint Diseases, SNP Research Center, RIKEN, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan.
Masahiro
M
Nakajima
The Center of Diagnosis and Treatment for Joint Disease, Drum Tower Hospital Affiliated to Medical School of Nanjing University, Nanjing, 210008, Jiangsu, China.
Jin
J
Dai
Department of Oncology, Drum Tower Hospital Affiliated to Medical School of Nanjing University, Nanjing, 210008, Jiangsu, China.
Jia
J
Wei
Department of Orthopaedics, Larissa, Greece and Institute of Biomedical Research and Technology (BIOMED), University of Thessalia, Medical School, Larissa, Greece.
Konstantinos N
KN
Malizos
The Center of Diagnosis and Treatment for Joint Disease, Drum Tower Hospital Affiliated to Medical School of Nanjing University, Nanjing, 210008, Jiangsu, China.
Jianghui
J
Qin
Laboratory for Bone and Joint Diseases, SNP Research Center, RIKEN, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan.
Yoshinari
Y
Miyamoto
Laboratory for Statistical Analysis, SNP Research Center, RIKEN, Tokyo, 108-8639, Japan.
Naoyuki
N
Kamatani
Department of Oncology, Drum Tower Hospital Affiliated to Medical School of Nanjing University, Nanjing, 210008, Jiangsu, China.
Baorui
B
Liu
Department of Biology, University of Thessalia, Medical School, Larissa, Greece.|Laboratory of Cytogenetics and Molecular Genetics, University Hospital of Larissa, University of Thessalia, Medical School, Larissa, Greece.
Aspasia
A
Tsezou
Laboratory for Mathematics, Premedical Course, National Defense Medical College, Saitama, 359-8513, Japan.
Takahiro
T
Nakamura
Laboratory for Bone and Joint Diseases, SNP Research Center, RIKEN, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan. sikegawa@ims.u-tokyo.ac.jp.
Shiro
S
Ikegawa
D000483:Alleles; D044466:Asian People; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006115:Greece; D006801:Humans; D008297:Male; D008840:Microfilament Proteins / Q000235:genetics*; D020370:Osteoarthritis, Knee / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide
C508643:LRCH1 protein, human; D008840:Microfilament Proteins