A pilot study of matrix metalloproteinases on the model of daunorubicin-induced cardiomyopathy in rabbits.
Matrix metalloproteinases (MMPs), activated by oxidative stress, play a key role during cardiac remodeling. In the present study we aimed to assess the role of MMPs in experimental cardiomyopathy induced by repeated 10-week administration of daunorubicin (3 mg/kg i.v.) to rabbits. In the daunorubicin group, the plasma cardiac troponin T levels (cTnT - a marker of myocardial necrosis) were significantly increased (p<0.05), commencing with the 8th administration compared with the controls. The amount of collagen (an estimate of fibrosis) was also significantly higher in the daunorubicin group (13.39 +/- 0.97 mg/g wet weight) compared to the control group (10.03 +/- 0.65 mg/g wet weight). In both groups, the LV MMP-activity was observed only in the gelatine substrate in the 70 kDa region (MMP-2), while no MMPs activities were detectable either in the casein or collagen containing zymograms. At the end of the experiment, the MMP-2 activity was slightly up-regulated (by 16 %) compared with the controls.
Department of Physiology, Charles University in Prague, Faculty of Medicine, University Hospital in Hradec Králové, Czech Republic. potacovaa@lfhk.cuni.cz
Anna
A
Potácová
Michaela
M
Adamcová
Martin
M
Sterba
Olga
O
Popelová
Tomás
T
Simůnek
Yvona
Y
Mazurová
Ivana
I
Guncová
Vladimír
V
Gersl
D000818:Animals; D009202:Cardiomyopathies / Q000139:chemically induced / Q000201:enzymology* / Q000378:metabolism / Q000503:physiopathology; D003094:Collagen / Q000378:metabolism; D003630:Daunorubicin / Q000633:toxicity*; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D011817:Rabbits; D020107:Troponin T / Q000378:metabolism; D020257:Ventricular Remodeling
D020107:Troponin T; D003094:Collagen; D020782:Matrix Metalloproteinases; D003630:Daunorubicin