Target metabolic profiling analysis of free amino acids in plasma as EOC/TBDMS derivatives by GC-SIM-MS.
Metabolic profiling analysis of free amino acids (AAs) in plasma (20 microL) was performed by gas chromatography-mass spectrometry in selected ion monitoring mode after ethoxycarbonyl/tert-butyldimethylsilyl derivatives. Characteristic fragment ions, including [M - 57](+) ions, permitted sensitive and selective detection of most of the AAs in the presence of co-extracted carboxylic acids, including free fatty acids, at much higher levels. The overall method was linear (r > or = 0.9991), reproducible (relative standard deviation = 2.3-8.8%) and accurate (relative error = -7.3-7.7%) with detection limits of 0.01-1.9 ng/mL. A total of 18 AAs, 15 protein AAs and three nonprotein AAs were quantitatively screened in a normal human plasma sample. This selective and simple method using a minimal sample volume was effective for the quantitation of plasma free AAs.
Metabolomic Analysis Laboratory, Institute for Neuroregeneration and Stem Cell Research, School of Medicine, Ajou University, Wonchon-dong, Yeongtong-gu, Suwon 443-721, South Korea.
Man-Jeong
MJ
Paik
Seung-Min
SM
Moon
Kyoung-Rae
KR
Kim
Sangdun
S
Choi
Young-Hwan
YH
Ahn
Gwang
G
Lee
D000596:Amino Acids / Q000097:blood* / Q000737:chemistry; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D009930:Organic Chemicals / Q000737:chemistry; D015203:Reproducibility of Results; D012821:Silanes / Q000737:chemistry
D000596:Amino Acids; D009930:Organic Chemicals; D012821:Silanes; C103256:tert-butyldimethylsilane