Antiproliferative activity of various Uncaria tomentosa preparations on HL-60 promyelocytic leukemia cells.
The woody Amazonian vine Uncaria tomentosa (cat's claw) has been recently more and more popular all over the world as an immunomodulatory, antiinflammatory and anti-cancer remedy. This study investigates anti-proliferative potency of several cat's claw preparations with different quantitative and qualitative alkaloid contents on HL-60 acute promyelocytic human cells by applying trypan blue exclusion and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction assay (MTT). By standardization and statistical comparison of the obtained results pteropodine and isomitraphylline are indicated to be most suitable for standardization of medical cat's claw preparations.
Pilarski
Radosław
R
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, PL 61-704 Poznań, Poland.
Poczekaj-Kostrzewska
Magdalena
M
Ciesiołka
Danuta
D
Szyfter
Krzysztof
K
Gulewicz
Krzysztof
K
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D020948:Cat's Claw; D004396:Coloring Agents; D004354:Drug Screening Assays, Antitumor; D018922:HL-60 Cells; D006801:Humans; D026121:Indole Alkaloids / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology; D000078183:Oxindoles; D028321:Plant Preparations / Q000494:pharmacology*; D013141:Spiro Compounds / Q000494:pharmacology; D013778:Tetrazolium Salts; D013844:Thiazoles; D014343:Trypan Blue
D000972:Antineoplastic Agents, Phytogenic; D004396:Coloring Agents; D026121:Indole Alkaloids; D007211:Indoles; D000078183:Oxindoles; D028321:Plant Preparations; D013141:Spiro Compounds; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue; D014343:Trypan Blue; C431785:uncarine C; C509329:isomitraphylline