Non-enzymatic covalent protein labeling using a reactive tag.
We describe herein a new method for covalent labeling of proteins using a complementary recognition pair of peptide tag and synthetic molecular probe. The rapid and specific covalent labeling of a tag-fused protein was achieved by the reaction on the tag site with the probe through their selective molecular recognition. The advantages of this method involve the facile functional modification and the high labeling specificity of the tag-fused protein, which are demonstrated in the labeling experiments in various conditions even inside cells.
Nonaka
Hiroshi
H
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto, 615-8510, Japan.
Tsukiji
Shinya
S
Ojida
Akio
A
Hamachi
Itaru
I
D004591:Electrophoresis, Polyacrylamide Gel; D029968:Escherichia coli Proteins / Q000737:chemistry*; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000737:chemistry; D015335:Molecular Probes; D012680:Sensitivity and Specificity
D029968:Escherichia coli Proteins; D015335:Molecular Probes; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins