An antennal circadian clock and circadian rhythms in peripheral pheromone reception in the moth Spodoptera littoralis.
Circadian rhythms are observed in mating behaviors in moths: females emit sex pheromones and males are attracted by these pheromones in rhythmic fashions. In the moth Spodoptera littoralis, we demonstrated the occurrence of a circadian oscillator in the antenna, the peripheral olfactory organ. We identified different clock genes, period (per), cryptochrome1 (cry1) and cryptochrome2 (cry2), in this organ. Using quantitative real-time PCR (qPCR), we found that their corresponding transcripts cycled circadianly in the antenna as well as in the brain. Electroantennogram (EAG) recordings over 24 h demonstrated for the first time a circadian rhythm in antennal responses of a moth to sex pheromone. qPCR showed that out of one pheromone-binding protein (PBP), one olfactory receptor (OR), and one odorant-degrading enzyme (ODE), all putatively involved in the pheromone reception, only the ODE transcript presented a circadian rhythm that may be related to rhythms in olfactory signal resolution. Peripheral or central circadian clock control of olfaction is then discussed in light of recent data.
Merlin
Christine
C
UMR 1272 INRA-UPMC-AgroParisTech "Physiologie de l'Insecte: Signalisation et Communication," INRA Centre de Versailles, Versailles cedex, France.
Lucas
Philippe
P
Rochat
Didier
D
François
Marie-Christine
MC
Maïbèche-Coisne
Martine
M
Jacquin-Joly
Emmanuelle
E
D000595:Amino Acid Sequence; D000818:Animals; D001683:Biological Clocks; D002940:Circadian Rhythm / Q000502:physiology*; D017931:DNA Primers; D003624:Darkness; D004563:Electrochemistry; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics; D007313:Insecta / Q000145:classification / Q000235:genetics; D008027:Light; D008297:Male; D051379:Mice / Q000145:classification / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D043563:Receptors, Pheromone / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D012903:Smell / Q000502:physiology; D018411:Spodoptera / Q000145:classification / Q000235:genetics / Q000502:physiology*
D017931:DNA Primers; D019476:Insect Proteins; D043563:Receptors, Pheromone