High specificity but contrasting biodiversity of Sphagnum-associated bacterial and plant communities in bog ecosystems independent of the geographical region.
Mosses represent ecological niches that harbor a hitherto largely uncharacterized microbial diversity. To investigate which factors affect the biodiversity of bryophyte-associated bacteria, we analyzed the bacterial communities associated with two moss species, which exhibit different ecological behaviors and importance in bog ecosystems, Sphagnum magellanicum and Sphagnum fallax, from six temperate and boreal bogs in Germany and Norway. Furthermore, their surrounding plant communities were studied. Molecular analysis of bacterial communities was determined by single-strand conformation polymorphism (SSCP) analysis using eubacterial and genus-specific primers for the dominant genera Burkholderia and Serratia as well as by sequence analysis of a Burkholderia 16S rRNA gene clone library. Plant communities were analyzed by monitoring the abundance and composition of bryophyte and vascular plant species, and by determining ecological indicator values. Interestingly, we found a high degree of host specificity for associated bacterial and plant communities of both Sphagnum species independent of the geographical region. Calculation of diversity indices on the basis of SSCP gels showed that the S. fallax-associated communities displayed a statistically significant higher degree of diversity than those associated with S. magellanicum. In contrast, analyses of plant communities of Sphagnum-specific habitats resulted in a higher diversity of S. magellanicum-specific habitats for all six sites. The higher content of nutrients in the S. fallax-associated ecosystems can explain higher diversity of microorganisms.
Institute of Environmental Biotechnology, University of Technology Graz, Graz, Austria.
Katja
K
Opelt
Christian
C
Berg
Susan
S
Schönmann
Leo
L
Eberl
Gabriele
G
Berg
D044822:Biodiversity; D019117:Burkholderia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D017753:Ecosystem; D005858:Germany; D008855:Microscopy, Electron, Scanning; D009664:Norway; D010802:Phylogeny; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D012705:Serratia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044003:Sphagnopsida / Q000382:microbiology* / Q000648:ultrastructure; D053833:Wetlands
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal