[Hemocompatibility of bovine pericardium with additional sodium bisulfite treatment].
OBJECTIVE
To evaluate the hemocompatibility of glutaraldehyde (GA)-tanned bovine pericardium additionally treated by sodium bisulfite (SOB) solution.


METHODS
The hemocompatibility of GA-tanned bovine pericardium treated by SOB solution is evaluated by using dynamic clotting time test, blood platelet adhension test, D-dimeride determination, and complement activation test. The GA-tanned bovine pericardium was used as control.


RESULTS
The curve of absorbance-clotting time of two kinds of bovine pericardium was similar in dynamic clotting time test. There was no significant difference between SOB-treated and control groups in blood platelet adhension test. The D-dimeride contents of all bioprostheses were at normal level, and the D-dimeride content of GA-tanned bovine pericardium treated by SOB solution was significantly lower than that of control group (P < 0.05). In complement activation test, the level of complement C3a in SOB-treated group was significantly lower than that in control group (P < 0.05).


CONCLUSIONS
GA-tanned bovine pericardium treated by SOB solution meets the demands of cardiac interstitial implanted materials in hemocompatibility.
Research Center for Cardiovascular Regeneration Medicine of the Ministry of Health, Cardiovascular Institute and Fuwai Hospital, CAMS and PUMC, Beijing 100037, China.
Mao-sheng
MS
Han
Jian-ye
JY
Zhou
Sheng-shou
SS
Hu
Sheng
S
Wang
Hong
H
Jiang
D000818:Animals; D001672:Biocompatible Materials; D001705:Bioprosthesis; D001777:Blood Coagulation; D002417:Cattle; D015926:Complement C3a / Q000032:analysis; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism; D005976:Glutaral / Q000494:pharmacology; D008422:Materials Testing; D010496:Pericardium / Q000187:drug effects* / Q000378:metabolism; D010973:Platelet Adhesiveness; D013447:Sulfites / Q000494:pharmacology*
D001672:Biocompatible Materials; D005338:Fibrin Fibrinogen Degradation Products; D013447:Sulfites; C036309:fibrin fragment D; D015926:Complement C3a; D005976:Glutaral; C009279:sodium bisulfite