Inhibitory properties of nucleotides with difluoromethylenephosphonic acid as a phosphate mimic versus calf spleen purine nucleoside phosphorylase and effect of these analogues on the viability of human blood lymphocytes.
Several cyclic and acyclic 6-keto purine nucleotides with difluoromethylenephosphonic acid as phosphate mimic are proved to be potent inhibitors of mammalian purine nucleoside phosphorylase (PNP). Antiproliferative activity of these analogues on the growth of human blood lymphocytes was tested by MTT assay. Compared to inhibitory effects on the growth of human blood T-lymphocytes isolated from healthy donors, all analogues significantly slow down proliferation of T-lymphocytes isolated from patients with autoimmune thyroid disease--Hashimoto's thyroiditis.
Glavas-Obrovac
Ljubica
L
Clinical Hospital Osijek and School of Medicine, University of J. J. Strossmayer in Osijek, Osijek, Croatia.
Suver
Mirjana
M
Hikishima
Sadao
S
Yokomatsu
Tsutomu
T
Bzowska
Agnieszka
A
D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015195:Drug Design; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D050031:Hashimoto Disease / Q000188:drug therapy / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects; D011685:Purine Nucleotides / Q000737:chemistry* / Q000494:pharmacology*; D011683:Purine-Nucleoside Phosphorylase / Q000037:antagonists & inhibitors*; D013154:Spleen / Q000201:enzymology
D004791:Enzyme Inhibitors; D011685:Purine Nucleotides; D011683:Purine-Nucleoside Phosphorylase