[Selection and inhibitory effect analysis of siRNAs specific to ORF2-4 of porcine reproductive and respiratory syndrome virus].
RNA interference (RNAi) is a powerful tool in gene function research. In order to investigate the role of GP2, GP3 and GP4 of porcine reproductive and respiratory syndrome virus (PRRSV) in the viral replication, small interference RNAs (siRNAs) directed to ORF2, ORF3 and ORF4 were designed and 12 short hairpin RNA (shRNA) expression vectors were constructed (designed as 21,22,23,24,31,32,33,34,41,42,43 and 44). Cells treated with shRNA expression vectors were infected by PRRSV. The effective shRNA expression vectors were selected by fluorescent quantatitive PCR (FQ-PCR). The virus titer of supernatant of the cells treated with effective shRNA expression vectors (23,24,31,34 and 41) were reduced by 184 to 4.65 folds compared with that of controls.
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Yun-Xia
YX
He
Rong-Hong
RH
Hua
Yan-Jun
YJ
Zhou
Tong-Qing
TQ
An
Hua-Ji
HJ
Qiu
Yun-Feng
YF
Wang
Guang-Zhi
GZ
Tong
D005822:Genetic Vectors; D016366:Open Reading Frames; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism*; D014161:Transduction, Genetic; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics*
D034741:RNA, Small Interfering; D012367:RNA, Viral; D014764:Viral Proteins