Effect of aging on fatty streak formation in a diet-induced mouse model of atherosclerosis.
Age is considered to be a major risk factor for atherosclerosis, but it is unclear whether age has a direct effect on susceptibility to atherosclerosis. Wild-type mice develop fatty streak lesions in the aortic root only when fed a cholate-containing high fat/cholesterol diet. To investigate the influence of age on fatty streak formation, young (10 weeks) and old (53 weeks) female C57BL/6 mice were fed an atherogenic diet containing 15% fat, 1.25% cholesterol and 0.5% sodium cholate for 12 weeks. Atherosclerotic lesions at the aortic root were measured after cryosections were stained with oil red O. Results showed that old mice developed a comparable size of aortic lesions with young counterparts (5,600 +/- 2,480 vs. 6,457 +/- 1,537 microm2/section; p = 0.77), although old mice had significantly higher plasma cholesterol levels than young mice on the atherogenic diet (p < 0.05). Plasma levels of soluble vascular cell adhesion molecule 1 were significantly higher in old mice than in young mice on both chow and Western diets (p < 0.005). These data indicate that age has no direct effect on atherosclerosis susceptibility although it is accompanied by elevations in plasma cholesterol and vascular cell adhesion molecule 1 levels in C57BL/6 mice. Thus, increased cardiovascular events with age are probably related to a progressive increase in plaque size rather than to an increase in atherosclerosis susceptibility.
Department of Radiology, University of Virginia, Charlottesville, Va 22908, USA.
Yuhua
Y
Li
Timothy R
TR
Gilbert
Alan H
AH
Matsumoto
Weibin
W
Shi
D000375:Aging / Q000097:blood / Q000378:metabolism; D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D001021:Aortic Valve / Q000473:pathology; D050197:Atherosclerosis / Q000097:blood / Q000150:complications* / Q000209:etiology* / Q000473:pathology; D002784:Cholesterol / Q000097:blood; D004036:Diet, Atherogenic; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D005260:Female; D008055:Lipids / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood / Q000378:metabolism
D008055:Lipids; D019010:Vascular Cell Adhesion Molecule-1; D002784:Cholesterol