Role of galK and galM in galactose metabolism by Streptococcus thermophilus.
Streptococcus thermophilus is unable to metabolize the galactose moiety of lactose. In this paper, we show that a transformant of S. thermophilus SMQ-301 expressing Streptococcus salivarius galK and galM was able to grow on galactose and expelled at least twofold less galactose into the medium during growth on lactose.
Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, Quebec, Canada G1K 7P4.
Katy
K
Vaillancourt
Nathalie
N
Bédard
Christian
C
Bart
Mélanie
M
Tessier
Gilles
G
Robitaille
Nathalie
N
Turgeon
Michel
M
Frenette
Sylvain
S
Moineau
Christian
C
Vadeboncoeur
D015152:Blotting, Northern; D015153:Blotting, Western; D002238:Carbohydrate Epimerases / Q000235:genetics* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D017931:DNA Primers / Q000235:genetics; D005686:Galactokinase / Q000235:genetics* / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D048249:Streptococcus thermophilus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014169:Transformation, Bacterial
D003470:Culture Media; D017931:DNA Primers; D005686:Galactokinase; D002238:Carbohydrate Epimerases; C459183:galactose mutarotase; D005690:Galactose