In vitro incorporation of LNA nucleotides.
An LNA modified nucleoside triphosphate 1 was synthesized in order to investigate its potential to act as substrate for DNA strand synthesis by polymerases. Primer extension assays for the incorporation experiments revealed that Phusion High Fidelity DNA polymerase is an efficient enzyme for incorporation of the LNA nucleotide and for extending strand to full length. It was also observed that pfu DNA polymerase could incorporate the LNA nucleotide but it failed to extend the strand to a full length product.
Veedu
Rakesh N
RN
Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Odense M, Denmark. rnv@chem.sdu.dk
Vester
Birte
B
Wengel
Jesper
J
D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D066298:In Vitro Techniques; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity
D017931:DNA Primers; D009841:Oligonucleotides; C477371:locked nucleic acid; D004247:DNA; D004259:DNA-Directed DNA Polymerase