Transposon mediated transgenesis in a marine invertebrate chordate: Ciona intestinalis.
Achievement of transposon mediated germline transgenesis in a basal chordate, Ciona intestinalis, is discussed. A Tc1/mariner superfamily transposon, Minos, has excision and transposition activities in Ciona. Minos enables the creation of stable transgenic lines, enhancer detection, and insertional mutagenesis.
Sasakura
Yasunori
Y
Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, 415-0025, Japan. sasakura@kurofune.shimoda.tsukuba.ac.jp
Oogai
Yuichi
Y
Matsuoka
Terumi
T
Satoh
Nori
N
Awazu
Satoko
S
D000818:Animals; D002938:Ciona intestinalis / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D018014:Gene Transfer Techniques; D016678:Genome; D016254:Mutagenesis, Insertional
D004251:DNA Transposable Elements