Phylogenetic analysis of betanodaviruses isolated from cultured fish in Korea.
Since the publication of the first report on fish nodaviruses in Korea in 1998, fish nodaviruses have caused widespread epizootic events among various fish species in Korea. However, the genotypes of fish nodaviruses in Korea have not yet been determined due to a lack of information about their nucleotide sequences. In this study, we isolated 5 fish nodaviruses from 4 fish species cultured in 4 different regions in Korea: rock bream Oplegnathus fasciatus, Japanese flounder Paralichthys olivaceus, sevenband grouper Epinephelus septemfasciatus, and grey mullet Mugil cophalus. The full open-reading frame (ORF) encoding the coat protein (1017 nt) was sequenced from each of the 5 fish nodaviruses and the nucleotide sequences were phylogenetically analyzed. Results showed that even though their sequences were not identical, all 5 Korean isolates were clustered in the RGNNV genotype. This is the first report on the phylogenetic analysis of fish nodaviruses from cultured fish in Korea.
Cha
Seung Ju
SJ
Pathology Division, National Fisheries Research & Development Institute, Kijang, Pusan 619-902, South Korea.
Do
Jeong Wan
JW
Lee
Nam Sil
NS
An
Eun Jeong
EJ
Kim
Yi Cheong
YC
Kim
Jin Woo
JW
Park
Jeong Woo
JW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000473:pathology; D036022:Capsid Proteins / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D005393:Fish Diseases / Q000821:virology*; D005398:Fisheries; D005412:Flatfishes / Q000821:virology; D007723:Korea; D008969:Molecular Sequence Data; D030381:Nodaviridae / Q000145:classification* / Q000302:isolation & purification; D010473:Perciformes / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012327:RNA Virus Infections / Q000662:veterinary* / Q000821:virology; D016415:Sequence Alignment; D023701:Smegmamorpha / Q000821:virology
D036022:Capsid Proteins; D017931:DNA Primers