Induction of oxalate decarboxylase by oxalate in a newly isolated Pandoraea sp. OXJ-11 and its ability to protect against Sclerotinia sclerotiorum infection.
Pandoraea sp. OXJ-11 has been shown to produce an oxalate decarboxylase. The enzyme could be induced by increasing the oxalate in the medium. An increasing concentration of yeast extract was able to stimulate the cell growth but could not increase the specific oxalate decarboxylase activity. The oxalate decarboxylase was produced maximally at 25-35 degrees C and pH 4.0-9.0, favoring its potential application in protection of host plants from oxalate-producing phytopathogens. The influence of glucose on the induction of oxalate decarboxylase by oxalate was examined, and it was found that glucose inhibited the production of the oxalate decarboxylase. Resistance results showed that Pandoraea sp. OXJ-11 was capable of suppressing Sclerotinia sclerotiorum infection on detached leaflets of Brassica napus plants.
Jin
Zhao-Xia
ZX
Faculty of Environment and Life, Dalian University of Technology, Dalian 116000, Peoples Republic of China.
Wang
Changhai
C
Chen
Wenfu
W
Chen
Xiaoyi
X
Li
Xianzhen
X
D001203:Ascomycota / Q000254:growth & development* / Q000378:metabolism; D029688:Brassica napus / Q000382:microbiology; D042521:Burkholderiaceae / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism; D002262:Carboxy-Lyases / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010070:Oxalates / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D012988:Soil Microbiology; D013696:Temperature; D013997:Time Factors
D010070:Oxalates; D002262:Carboxy-Lyases; C021915:oxalate decarboxylase; D005947:Glucose