Human mulipotential mesenchymal/stromal stem cells are derived from a discrete subpopulation of STRO-1bright/CD34 /CD45(-)/glycophorin-A-bone marrow cells.
Magnetic and flow cytometry-based methods were used to characterize clonogenic stromal cells in human bone marrow. STRO-1(bright) stromal cells were found to lack expression of CD34, CD45 and glycophorin-A markers associated with hematopoietic progenitor cells. These studies support the view that these are two distinct stem cell compartments in adult bone marrow.
Multiple Myeloma Research Laboratory, Division of Haematology, Institute of Medical and Veterinary Science, Hanson Institute and University of Adelaide, Australia.
Andrew C W
AC
Zannettino
Sharon
S
Paton
Angela
A
Kortesidis
Fiona
F
Khor
Silviu
S
Itescu
Stan
S
Gronthos
D018952:Antigens, CD34; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D001853:Bone Marrow; D005434:Flow Cytometry; D006021:Glycophorins; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D018189:Immunomagnetic Separation; D017493:Leukocyte Common Antigens; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D039902:Multipotent Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology
D018952:Antigens, CD34; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D006021:Glycophorins; C525591:STRO-1 antigen, human; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human