Is the use of fullerene in photodynamic therapy effective for atherosclerosis?
The purpose of this study was to evaluate Fullerene as a therapeutic photosensitizer in the treatment of atherosclerosis. An atherosclerotic experimental rabbit model was prepared by causing intimal injury to bilateral external iliac arteries using balloon expansion. In four atherosclerotic rabbits and one normal rabbit, polyethylene glycol-modified Fullerene (Fullerene-PEG) was infused into the left external iliac artery and illuminated by light emitting diode (LED), while the right external iliac artery was only illuminated by LED. Two weeks later, the histological findings for each iliac artery were evaluated quantitatively and comparisons were made among atherosclerotic Fullerene+LED artery (n = 4), atherosclerotic light artery (n = 4), normal Fullerene+LED artery (n = 1), and normal light artery (n = 1). An additional two atherosclerotic rabbits were studied by fluorescence microscopy, after Fullerene-PEG-Cy5 complex infusion into the left external iliac artery, for evaluation of Fullerene-PEG incorporated within the atherosclerotic lesions. The degree of atherosclerosis in the atherosclerotic Fullerene+LED artery was significantly (p < 0.05) more severe than that in the atherosclerotic LED artery. No pathological change was observed in normal Fullerene+LED and LED arteries. In addition, strong accumulation of Fullerene-PEG-Cy5 complex within the plaque of the left iliac artery of the two rabbits was demonstrated, in contrast to no accumulation in the right iliac artery. We conclude that infusion of a high concentration of Fullerene-PEG followed by photo-illumination resulted not in a suppression of atherosclerosis but in a progression of atherosclerosis in experimental rabbit models. However, this intervention showed no adverse effects on the normal iliac artery.
Department of Radiology, Shiga University of Medical Science, Tsukinowa-cho, Otsu, Shiga, 520-2192, Japan. r34nitta@belle.shiga-med.ac.jp
Norihisa
N
Nitta
Ayumi
A
Seko
Akinaga
A
Sonoda
Shinichi
S
Ohta
Toyohiko
T
Tanaka
Masashi
M
Takahashi
Kiyoshi
K
Murata
Shizuki
S
Takemura
Tsutomu
T
Sakamoto
Yasuhiko
Y
Tabata
D000818:Animals; D050197:Atherosclerosis / Q000188:drug therapy*; D004195:Disease Models, Animal; D037741:Fullerenes / Q000494:pharmacology*; D010778:Photochemotherapy / Q000379:methods*; D011092:Polyethylene Glycols / Q000494:pharmacology; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000378:metabolism
D037741:Fullerenes; D017382:Reactive Oxygen Species; D011092:Polyethylene Glycols