[Inhibition effects of constituents of Radix Salviae Miltiorrhizae on proliferation and procollagen transcription of dermal fibroblasts in systemic sclerosis].
OBJECTIVE
To study the effects of tanshinone IIA, danshensu, lithospermate, and protocatechualdehyde on proliferation and procollagen mRNA synthesis of dermal fibroblasts from patients with systemic sclerosis (SSc).


METHODS
Dermal fibroblasts were obtained from 5 patients with SSc, 2 males and 3 females, and sex and age-matched 5 patients undergoing plastic surgery, cultured, and then co-cultured with tanshinone IIA, danshensu, lithospermate, and protocatechualdehyde respectively. MTT test was used to study the effects of the four constituents on fibroblasts cell proliferation. RT-PCR was used to detect the level of procollagen mRNA expression in the control fibroblasts and in the SSc fibroblasts before and after the addition of the four constituents.


RESULTS
The 4 constituents all suppressed the proliferation of fibroblasts from SSc patients, with tanshinone IIA being the most effective. The 4 constituents remarkably inhibited the mRNA expression of type I and III procollagen of the fibroblasts with tanshinone II A showing the most inhibition effect that reduced the expressions of procollagen I mRNA by 47% and procollagen III mRNA by 45%.


CONCLUSIONS
Of the four constituents, tanshinone IIA is the most suppressive effect on the cell proliferation and type I and type III procollagen synthesis of SSc fibroblasts, demonstrating that it has a more antifibrotic effect.
Lü
Xiao-yan
XY
Department of Dermatology, West China Hospital, Sichuan University, Chengdu 610041, China.
Li
Ming
M
Weng
Meng-wu
MW
D045784:Abietanes; D001547:Benzaldehydes / Q000494:pharmacology; D002396:Catechols / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics; D024061:Collagen Type III / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007773:Lactates / Q000494:pharmacology; D008297:Male; D010616:Phenanthrenes / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011347:Procollagen / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027561:Salvia miltiorrhiza; D012595:Scleroderma, Systemic / Q000235:genetics / Q000473:pathology*; D014158:Transcription, Genetic / Q000187:drug effects
D045784:Abietanes; D001547:Benzaldehydes; D002396:Catechols; D024042:Collagen Type I; D024061:Collagen Type III; D004365:Drugs, Chinese Herbal; D007773:Lactates; D010616:Phenanthrenes; D010936:Plant Extracts; D011347:Procollagen; D012333:RNA, Messenger; C021751:tanshinone; C061091:lithospermate B; C035055:3,4-dihydroxyphenyllactic acid; C005581:protocatechualdehyde