An optimized immunohistochemical method for detection of phosphorylated mitogen-activated protein kinases.
Study of the in vivo spatio-temporal localization of modified proteins is likely to become a major focus of proteomics research in the near future. In this study we optimized and tested an immunohistochemical procedure for detecting unstable phosphorylated mitogen-activated protein (MAP) kinases. Using our method, phosphorylated MAP kinases can be sensitively and reproducibly localized in the developing white matter of murine spinal cord on embryonic day 15. Our method is simple and effective, and so may be useful in future proteomics research.
Department of Psychiatry, National Defense Medical College, Tokorozawa, Saitama, Japan.
Toshiya
T
Teraishi
Kenji
K
Miura
Junko
J
Imaki
D000818:Animals; D005629:Frozen Sections; D005865:Gestational Age; D022781:Hepatocytes / Q000201:enzymology; D007150:Immunohistochemistry; D051379:Mice; D008813:Mice, Inbred ICR; D019950:Mitogen-Activated Protein Kinase 1 / Q000032:analysis; D048052:Mitogen-Activated Protein Kinase 3 / Q000032:analysis; D048055:Mitogen-Activated Protein Kinase 8 / Q000032:analysis; D048056:Mitogen-Activated Protein Kinase 9 / Q000032:analysis; D020928:Mitogen-Activated Protein Kinases / Q000032:analysis*; D016612:Paraffin Embedding; D010766:Phosphorylation; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D013116:Spinal Cord / Q000196:embryology / Q000201:enzymology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000032:analysis
D048056:Mitogen-Activated Protein Kinase 9; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D048055:Mitogen-Activated Protein Kinase 8; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases