Isolation and characterization of antioxidation enzymes from cells of zedoary (Curcuma zedoaria Roscoe) cultured in a 5-l bioreactor.
In this study, a cell suspension culture system for zedoary (Curcuma zedoaria Roscoe) was developed, using 50 g/l of fresh weight inoculum in a batch culture. The highest cell biomass obtained from a 5-l bioreactor equipped with three impellers after 14 days of culture was utilized to extract secondary metabolites (essential oil and curcumin) and determine the activities of antioxidant enzymes (peroxidase, superoxide dismutase, and catalase). For essential oil and curcumin, zedoary extracts were recovered via a variety of methods: steam distillation, volatile solvents, and Soxhlet. After 14 days of culture using volatile solvents, the optimal yield of essential oil (1.78%) was obtained when using petroleum ether at 40 degrees C in 6 h of extraction, and the best curcumin yield (9.69%) was obtained at 60 degrees C in 6 h via extraction with 90% ethanol. The activities of antioxidant enzymes from zedoary cells were also assessed. The specific activities of peroxidase, superoxide-dismutase, and catalase reached maximum values of 0.63 U/mg of protein, 16.60 U/mg of protein, and 19.59 U/mg of protein after 14 days of culture, respectively.
College of Sciences and Centre for Resource-Environment and Biotechnology, Hue University, Hue, Vietnam.
Nguyen-Hoang
NH
Loc
Doan-Thi-Hong
DT
Diem
Doan-Huu-Nhat
DH
Binh
Dao-Thi
DT
Huong
Tae-Geum
TG
Kim
Moon-Sik
MS
Yang
D000975:Antioxidants / Q000302:isolation & purification*; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology; D002374:Catalase / Q000302:isolation & purification; D002478:Cells, Cultured; D030024:Curcuma / Q000201:enzymology* / Q000254:growth & development; D004798:Enzymes / Q000302:isolation & purification*; D009195:Peroxidase / Q000302:isolation & purification; D010938:Plant Oils / Q000302:isolation & purification; D013482:Superoxide Dismutase / Q000302:isolation & purification
D000975:Antioxidants; D004798:Enzymes; D010938:Plant Oils; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase