Further studies in the developmental hyperserotonemia model (DHS) of autism: social, behavioral and peptide changes.
Prior research has reliably found high blood (hyperserotonemia) - but low brain - serotonin levels in autistic individuals. At early stages of development, high levels of serotonin in the blood may enter the brain of a developing fetus, causing a loss of serotonin terminals through negative feedback and thus disrupting subsequent serotonergic function. The current study extends earlier findings in a developmental hyperserotonemia (DHS) model of autism in Sprague-Dawley rats by treating 8 dams of developing rat pups with a serotonergic agonist, 5-methoxytryptamine (5-MT; 1 mg/kg) during development (from gestational day 12 to post-natal day 20; PND 20). DHS pups exhibited post-injection seizures, which were non-existent in saline-treated pups (p<0.05). Behavioral results in infancy indicated that DHS pups spent less time with the dam during the active phase on PNDs 15-17 (p<0.05) and experienced decreased maternal bonding in a return to dam task on PND 17 (p<0.05). On subsequent tests, DHS animals exhibited greater gnawing reactions to a novel stimulus (p<0.05), less behavioral inhibition (p<0.05), and had fewer olfactory-based social interactions (p<0.05) and greater non-olfactory mounting (p<0.05). However, there were no changes in anxiogenic behavior using the elevated plus maze (p>0.05). Post mortem analyses revealed that DHS animals had a loss of oxytocin (OT)-containing cells in the paraventricular nucleus in the hypothalamus (PVN; p<0.05) as well as an increase in calcitonin-gene related peptide (CGRP; p<0.05, one tailed) processes in the central nucleus of the amygdala (CeA) on PND 198. These results may correspond to hypothalamic and amygdalar changes in the human condition and suggest that the hyperserotonemia model of autism may be a valid model which produces many of the social, behavioral, and peptide changes inherent to autism.
McNamara
Ilan M
IM
State University of New York at Stony Brook, New York 11794-2500, USA. imcnamar@ic.sunysb.edu
Borella
Alice W
AW
Bialowas
Lynn A
LA
Whitaker-Azmitia
Patricia M
PM
D000679:Amygdala / Q000378:metabolism / Q000503:physiopathology; D000818:Animals; D000831:Animals, Newborn; D001008:Anxiety Disorders / Q000378:metabolism / Q000503:physiopathology; D001321:Autistic Disorder / Q000097:blood* / Q000209:etiology / Q000503:physiopathology; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000254:growth & development / Q000378:metabolism* / Q000503:physiopathology; D001928:Brain Diseases, Metabolic / Q000097:blood* / Q000139:chemically induced / Q000503:physiopathology; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D004195:Disease Models, Animal; D005106:Exploratory Behavior / Q000187:drug effects / Q000502:physiology; D007150:Immunohistochemistry; D008425:Maternal Behavior / Q000502:physiology; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D009479:Neuropeptides / Q000378:metabolism*; D010121:Oxytocin / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000097:blood*; D017366:Serotonin Receptor Agonists / Q000633:toxicity; D012920:Social Behavior Disorders / Q000097:blood* / Q000139:chemically induced / Q000503:physiopathology
D009479:Neuropeptides; D017366:Serotonin Receptor Agonists; D012701:Serotonin; D010121:Oxytocin; D015740:Calcitonin Gene-Related Peptide