[Determination of macrozamin in Rhizoma Heterosmilacis Japonicae by RP-HPLC].
OBJECTIVE
To develop a RP-HPLC method for the determination of the content of macrozamin in Rhizoma Heterosmilacis Japonicae.


METHODS
A Century C18 AQ column (4.6 mm x 250 mm, 5 microm) was used with the mobile phase consisted of water (4:96). The flow rate was 1.0 mL x min(-1). The detection wavelength was set at 215 nm, and the column temperature was 35 degrees C.


RESULTS
The calibration curve was linear (r = 0.999 8) in the range of 19.12 - 382.4 microg x mL(-1) for macrozamin, the average recovery of the method was 99.5%, with RSD 2.1% (n = 9).


CONCLUSIONS
This method can be used for the quality study of Rhizoma Heterosmilacis Japonicae.
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Lei
L
Qiao
Xiao-Hui
XH
Chen
Kai-Shun
KS
Bi
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019668:Liliaceae / Q000737:chemistry*; D008746:Methylazoxymethanol Acetate / Q000031:analogs & derivatives* / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry*
D008746:Methylazoxymethanol Acetate; C008305:macrozamin