In vitro production of llama (Lama glama) embryos by IVF and ICSI with fresh semen.
The interest for South American camelids has increased in the last years. The aim of the present research was to compare the in vitro production of Lama glama embryos using two techniques: in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). For IVF technique, we compared the effect of adding or not, heparin, penicillamine and hypotaurine as sperm capacitating agents. In the oocyte group subjected to ICSI, activation with or without, ionomycin and 6-dimethylaminopurine (6-DMAP) was assessed. Semen samples were obtained by electroejaculation and incubated at 38 degrees C in a 25% (v/v) collagenase solution. The cleavage and embryo development rates were compared between the different experimental groups. Only the number of cleaved oocytes was less when ICSI with no activation was used (p<0.05).
Conde
Pamela A
PA
Halitus Biotecnología, Halitus Instituto Medico, Capital Federal, Argentina. pamela.conde@halitus.com
Herrera
Carolina
C
Trasorras
Virginia L
VL
Giuliano
Susana M
SM
Director
Ariel
A
Miragaya
Marcelo H
MH
Chaves
María G
MG
Sarchi
María I
MI
Stivale
Diana
D
Quintans
Carlos
C
Agüero
Alicia
A
Rutter
Bruno
B
Pasqualini
Sergio
S
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001118:Argentina; D002161:Camelids, New World / Q000502:physiology*; D004542:Ejaculation; D004558:Electric Stimulation; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods* / Q000662:veterinary; D006493:Heparin / Q000494:pharmacology; D015759:Ionomycin / Q000494:pharmacology; D008297:Male; D054315:Oocyte Retrieval; D010396:Penicillamine / Q000494:pharmacology; D011247:Pregnancy; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D012661:Semen / Q000502:physiology*; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods* / Q000662:veterinary; D013654:Taurine / Q000031:analogs & derivatives / Q000494:pharmacology
D000975:Antioxidants; D047428:Protein Kinase Inhibitors; D013654:Taurine; D015759:Ionomycin; C003949:hypotaurine; C001020:N(6),N(6)-dimethyladenine; D006493:Heparin; D010396:Penicillamine; D000225:Adenine