Expression of interleukin-17 in lung and peripheral blood of asthmatic rats and the influence of dexamethasone.
The expression of interleukin-17 (IL-17) in lung and peripheral blood of asthmatic rats and the influence of dexamethasone, and the role of IL-17 in the pathogenesis of asthma were investigated. Thirty Sprague-Dawley (SD) adult rats were randomly divided into three groups (n=10 in each group): normal group, asthmatic group, and dexamethasone-interfered group. Rat asthmatic model was established by intraperitoneal (i.p.) injection of 10% ovalbumin (OVA) and challenge with 1% OVA via inhalation. Rats in dexamethasone-interfered group were pretreated with dexamethasone (2 mg/kg, i.p.) 30 min before each challenge. The expression of IL-17 protein in serum and bronchoalveolar lavage fluid (BALF) was detected by ELISA. The expression of IL-17 mRNA in peripheral blood mononuclear cells (PBMC) and BALF cells was semi-quantitatively detected by RT-PCR. The expression of IL-17 protein in serum and BALF of asthmatic rats was significantly elevated as compared with normal rats and dexamethasone-interfered rats (P<0.01), and there was significant difference between normal rats and dexamethasone-interfered rats (P<0.05). The expression of IL-17 mRNA in PBMC and BALF cells of asthmatic rats was markedly increased as compared with normal rats and dexamethasone-interfered rats (P<0.01), and significant difference was found between normal rats and dexamethasone-interfered rats (P<0.05). It was concluded that the expression of IL-17 was increased significantly in asthmatic rats and could be inhibited partly by dexamethasone, suggesting that IL-17 might play an important role in the pathogenesis of asthma as an inflammation regulation factor.
Xiong
Weining
W
Department of Respiratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. xiongweining@tjh.tjmu.edu.cn
Zeng
Daxiong
D
Xu
Yongjian
Y
Fang
Huijuan
H
Cao
Yong
Y
Song
Qingfeng
Q
Cao
Chao
C
D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D003907:Dexamethasone / Q000627:therapeutic use*; D020381:Interleukin-17 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D010047:Ovalbumin; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
C515934:Il17a protein, rat; D020381:Interleukin-17; D012333:RNA, Messenger; D003907:Dexamethasone; D010047:Ovalbumin