Comparative effects of copper, iron, vanadium and titanium on low density lipoprotein oxidation in vitro.
Oxidation of low density lipoprotein (LDL) has been strongly implicated in the phathogenesis of atherosclerosis. The use of oxidants in dietary food stuff may lead to the production of oxidized LDL and may increase both the development and the progression of atherosclerosis. The present work investigated the effects of some elements including: copper (Cu), iron (Fe), vanadium (V) and titanium (Ti) on in vitro LDL oxidation quantitatively.



The first LDL fraction was isolated from fresh plasma by single vertical discontinuous density gradient ultracentrifugation. The formation of conjugated dienes and thiobarbituric acid reactive substances and increase in electrophoretic mobility of LDL were monitored as markers of the oxidation of LDL.



It was demonstrated that Cu, Fe, V and Ti exhibited strong oxidant activity in this respect (P<0.001). Oxidation of LDL in the presence of Cu was more and appeared to be in this order Cu>Fe>V>Ti.



Cu, Fe, V and Ti are redox-active transition metals that may cause oxidative damage to lipids, proteins and DNA molecules. We suggest that these elements may also influence the oxidation of LDL in vivo, which could increase both the development and progression of atherosclerosis.
Dept. of Clinical Biochemistry, School ofPharmacy, Isfahan University of Medical Sciences, Isfahan, Iran.
Mohsen
M
Ani
Dept. of Clinical Biochemistry, School ofPharmacy, Isfahan University of Medical Sciences, Isfahan, Iran.
Ali Asghar
AA
Moshtaghie
Dept. of Clinical Biochemistry, School of Medicine, Lorestan University of Medical Sciences, Khoramabad, Iran.
Hassan
H
Ahmadvand
D019327:Copper Sulfate / Q000494:pharmacology*; D005290:Ferric Compounds / Q000494:pharmacology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D014025:Titanium / Q000494:pharmacology*; D017968:Vanadium Compounds / Q000494:pharmacology*
D005290:Ferric Compounds; D008077:Lipoproteins, LDL; D017968:Vanadium Compounds; C024823:ferric sulfate; C066075:vanadium pentoxide; D014025:Titanium; D019327:Copper Sulfate