Effect of heat damage in an autoclave on the reactive lysine contents of soy products and corn distillers dried grains with solubles. Use of the results to check on lysine damage in common qualities of these ingredients.
The suitability of the homoarginine reaction for determining the reactive lysine in soy products and corn distillers dried grain with solubles (DDGS) was tested. For this purpose, some batches were subjected to deliberate heat damage for up to 30 min in an autoclave with 135 degrees C hot steam, and the samples were analyzed for total lysine and reactive lysine. In addition, 84 samples of common soy and 80 samples of corn DDGS were tested for their content of total and reactive lysine, and the contents were compared with those of the autoclave tests. For soy products conclusive results were obtained. In the case of heat treatment, both total lysine and reactive lysine decrease, but the latter is clearly a more sensitive indicator of lysine damage. Most normal products are quite similar, with toasting-induced damage to reactive lysine of ca. 15% compared to untoasted beans. The cause of the constantly occurring residual lysine after guanidination and the poorer reaction balance in the case of damage were explained. For common DDGS samples, however, less favorable results were obtained. Reactive and total lysine decreased almost in parallel due to heat damage, showing a great gap between them. Results showed indeed that variation of total and reactive lysine in DDGS is high, proving that its production conditions are not yet optimal for a feed ingredient.
Feed Additives, Animal Nutrition Services, Evonik Degussa GmbH, Hanau, Germany. johannes.fontaine@evonik.com
Johannes
J
Fontaine
Ulrike
U
Zimmer
Paul J
PJ
Moughan
Shane M
SM
Rutherfurd
D000821:Animal Feed / Q000032:analysis; D002523:Edible Grain / Q000737:chemistry*; D019791:Guanidine / Q000737:chemistry; D006358:Hot Temperature; D008239:Lysine / Q000032:analysis* / Q000737:chemistry*; D011312:Pressure; D045730:Soy Foods / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*
D019791:Guanidine; D008239:Lysine