Ferutinin stability in human plasma and interaction with human serum albumin.
Ferutinin is a potent phytoestrogen extracted from plants of the genus Ferula. The biological activity of this sesquiterpene is associated with the esterification of p-hydroxybenzoic acid with the daucane alcohol, jaeschkeanadiol. A HPLC method was developed to investigate the stability of ferutinin in acidic and basic solutions (pH 1.5 and 9.0, respectively), in buffer (pH 7.4) as well as in serial dilutions of albumin and in human plasma. The degradation of ferutinin was relatively slow at physiological pH 7.4 compared with low or high pH. Ferutinin was fully stable in human plasma as well as in albumin solution and the stability increased with albumin concentration. The binding of ferutinin to albumin was investigated by fluorescence spectroscopy. Ferutinin decreased the fluorescence of HSA and that of the only tryptophan residue located in domain IIA. As a result of the interaction between ferutinin and albumin, the binding of bilirubin decreased. The stability of ferutinin in plasma is attributable to ferutinin-albumin binding.
Holy Spirit University of Kaslik, Faculty of Sciences and Computer Engineering, B.P. 446 Jounieh, Lebanon. helenegreige@usek.edu.lb
H
H
Greige-Gerges
Y
Y
Diab
J
J
Farah
J
J
Magdalou
C
C
Haddad
N
N
Ouaini
D001565:Benzoates / Q000737:chemistry* / Q000378:metabolism; D001663:Bilirubin / Q000378:metabolism; D001665:Binding Sites; D001643:Bridged Bicyclo Compounds / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003508:Cycloheptanes / Q000737:chemistry* / Q000378:metabolism; D004355:Drug Stability; D005453:Fluorescence; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D012717:Sesquiterpenes / Q000737:chemistry* / Q000378:metabolism
D001565:Benzoates; D001643:Bridged Bicyclo Compounds; D003508:Cycloheptanes; D012709:Serum Albumin; D012717:Sesquiterpenes; C111068:4-oxy-6-(4-oxybezoyloxy)dauc-8,9-en; D001663:Bilirubin