Temperature effects on Korean entomopathogenic nematodes, Steinernema glaseri and S. longicaudum, and their symbiotic bacteria.
We investigated the temperature effects on the virulence, development, reproduction, and motility of two Korean isolates of entomopathogenic nematodes, Steinernema glaseri Dongrae strain and S. longicaudum Nonsan strain. In addition, we studied the growth and virulence of their respective symbiotic bacterium, Xenorhabdus poinarii for S. glaseri and Xenorhabdus sp. for S. longicaudum, in an insect host at different temperatures. Insects infected with the nematode-bacterium complex or the symbiotic bacterium was placed at 13 degrees C, 18 degrees C, 24 degrees C, 30 degrees C, or 35 degrees C in the dark and the various parameters were monitored. Both nematode species caused mortality at all temperatures tested, with higher mortalities occurring at temperatures between 24 degrees C and 30 degrees C. However, S. longicaudum was better adapted to cold temperatures and caused higher mortality at 18 degrees C than S. glaseri. Both nematode species developed to adult at all temperatures, but no progeny production occurred at 13 degrees C or 35 degrees C. For S. glaseri, nematode progeny production was best at inocula levels above 20 infective juveniles/host at 24 degrees C and 30 degrees C, but for S. longicaudum, progeny production was generally better at 24 degrees C. Steinernema glaseri showed the greatest motility at 30 degrees C, whereas S. longicaudum showed good motility at 24 degrees C and 30 degrees C. Both bacterial species grew at all tested temperatures, but Xenorhabdus sp. was more virulent at low temperatures (13 degrees C and 18 degrees C) than X poinarii.
Division of Applied Life Science, Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea.
Thi Dao
TD
Hang
Ho Yul
HY
Choo
Dong Woon
DW
Lee
Sang Myeong
SM
Lee
Harry K
HK
Kaya
Chung Gyoo
CG
Park
D000818:Animals; D001709:Biotechnology; D007723:Korea; D007814:Larva / Q000254:growth & development / Q000382:microbiology / Q000469:parasitology; D009036:Moths / Q000254:growth & development / Q000382:microbiology / Q000469:parasitology*; D009068:Movement; D010572:Pest Control, Biological; D017168:Rhabditida / Q000254:growth & development / Q000382:microbiology* / Q000472:pathogenicity / Q000502:physiology*; D013559:Symbiosis; D013696:Temperature; D014774:Virulence; D020593:Xenorhabdus / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*