Heparin-conjugated star-shaped PLA for improved biocompatibility.
Star-shaped PLA-heparin (sPLA-Hep) was prepared by coupling heparin to the star-shaped PLA (sPLA) reaction using carbonyldiimidazole (CDI) chemistry. Hydroxyl groups of sPLA were activated by CDI for the reaction with the remained amino-terminal groups of heparin. The surface heparin content of sPLA-Hep was measured to be 1.43 microg/cm(2). sPLA-Hep-coated surface has shown higher hydrophilicity than control surface. The clotting time of sPLA-Hep measured by activated partial thromboplastin time (APTT) was significantly prolonged when compared with sPLA and linear PLA-Hep. sPLA-Hep surface demonstrated lower protein adsorption and platelet adhesion than control sPLA surface. In addition, fibroblast culture on the sPLA-Hep surface showed the enhanced cell spreading area compared with the sPLA surface. Obtained results suggest that the incorporation of heparin to sPLA is effective in curtailing the surface induced-thrombosis and in manipulating the cell interaction. sPLA-Hep could be applied as blood/tissue compatible biodegradable materials for implantable medical devices and tissue engineering.
Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Korea.
Dong Hyun
DH
Go
Yoon Ki
YK
Joung
Sun Young
SY
Park
Yong Doo
YD
Park
Ki Dong
KD
Park
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D002465:Cell Movement; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D005455:Fluorescent Antibody Technique; D006493:Heparin / Q000032:analysis / Q000138:chemical synthesis / Q000378:metabolism*; D006801:Humans; D019344:Lactic Acid / Q000138:chemical synthesis / Q000378:metabolism*; D008422:Materials Testing; D051379:Mice; D010314:Partial Thromboplastin Time; D010973:Platelet Adhesiveness; D011091:Polyesters; D011108:Polymers / Q000138:chemical synthesis / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013552:Swine
D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D011506:Proteins; D019344:Lactic Acid; C033616:poly(lactide); D006493:Heparin