Inverse polarization transfer for detecting in vivo 13C magnetization transfer effect of specific enzyme reactions in 1H spectra.
The wide chemical shift dispersion and long T(1) of (13)C have allowed determination of in vivo magnetization transfer effects caused by aspartate aminotransferase and lactate dehydrogenase reactions using (13)C magnetic resonance spectroscopy. In this report, we demonstrate that these effects can be observed in the proton spectra by transferring the equilibrium magnetization of (13)C via the one-bond scalar coupling between (13)C and (1)H using an inverse insensitive nuclei enhanced by polarization transfer-based heteronuclear polarization transfer method. This inverse method allows a combination of the advantages of the long (13)C T(1) for maximum magnetization transfer and the high sensitivity of proton detection. The feasibility of this in vivo inverse polarization transfer approach was evaluated for detecting the (13)C magnetization transfer effect of aspartate aminotransferase and lactate dehydrogenase reactions from a 72.5-microl voxel in the rat brain at 11.7 T.
Molecular Imaging Branch, National Institute of Mental Health, Bethesda, MD 20892-1527, USA.
Su
S
Xu
Jehoon
J
Yang
Jun
J
Shen
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002247:Carbon Isotopes; D006859:Hydrogen; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012815:Signal Processing, Computer-Assisted
D002247:Carbon Isotopes; D006859:Hydrogen; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases