B cell lymphomas express CX3CR1 a non-B cell lineage adhesion molecule.
To study the differential expression of cell membrane-bound receptors and their potential role in growth and/or survival of the tumor cells, highly purified follicular lymphoma cells were analyzed, using gene expression analysis, and compared to non-malignant B cell populations. Filtering the genome for overexpressed genes coding for cell membrane-bound proteins/receptors resulted in a hit list of 27 identified genes. Among these, we have focused on the aberrant over expression of CX3CR1, in different types of B cell lymphoma, as compared to non-malignant B cells. We show that CX3CR1, which normally is not expressed on B cells, is expressed both at the mRNA and protein level in several subtypes of lymphoma. CX3CR1 has also shown to be involved in the homing to specific tissues that express the ligand, CX3CL1, in breast and prostate cancer and may thus be involved in dissemination of lymphoma.
Department of Immunotechnology, Lund University, CREATE Health, Lund University, BMC D13, 221 84, Lund, Sweden.
Ulrika
U
Andréasson
Sara
S
Ek
Hartmut
H
Merz
Richard
R
Rosenquist
Niels
N
Andersen
Mats
M
Jerkeman
Michael
M
Dictor
Carl A K
CA
Borrebaeck
D001402:B-Lymphocytes / Q000276:immunology*; D000073883:CX3C Chemokine Receptor 1; D002469:Cell Separation; D005434:Flow Cytometry; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008224:Lymphoma, Follicular / Q000235:genetics / Q000276:immunology*; D020522:Lymphoma, Mantle-Cell / Q000235:genetics / Q000276:immunology*; D020411:Oligonucleotide Array Sequence Analysis; D014066:Palatine Tonsil / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D019707:Receptors, Chemokine / Q000032:analysis* / Q000235:genetics
D000073883:CX3C Chemokine Receptor 1; C095235:CX3CR1 protein, human; D012333:RNA, Messenger; D019707:Receptors, Chemokine