Repeated batch production of epothilone B by immobilized Sorangium cellulosum.
Production of extracellular epothilone B, one of the potent anticancer agents, by free and immobilized Sorangium cellulosum was studied using the repeated batch culture process. The concentration of alginate used in immobilization was directly related to the mass transfer rate of nutrients, mechanical stability, and the epothilone B production yield. With the optimized 3% (w/v) calcium alginate carrier, a prolonged repeated batch culture was investigated for the 5 repeated batches for 24 days. The maximum productivity of epothilone B obtained from the alginate-immobilized cells was 5.03 mg/l/day, which is 3 times higher than that of free cells (1.68 mg/l/day).
Department of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Korea.
Woo Sang
WS
Park
Su Jeong
SJ
Park
Se Jong
SJ
Han
Jinwon
J
Lee
Dong-Shik
DS
Kim
Ji-Heung
JH
Kim
Byung Woo
BW
Kim
Jeewon
J
Lee
Sang Jun
SJ
Sim
D000464:Alginates / Q000737:chemistry / Q000648:ultrastructure; D000970:Antineoplastic Agents / Q000138:chemical synthesis*; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods; D018914:Cells, Immobilized / Q000378:metabolism*; D034261:Epothilones / Q000096:biosynthesis*; D009231:Myxococcales / Q000166:cytology* / Q000378:metabolism*; D013997:Time Factors
D000464:Alginates; D000970:Antineoplastic Agents; D034261:Epothilones; C093788:epothilone B