Voxel-based analysis of R2* maps in the healthy human brain.
OBJECTIVE
To develop a voxel-based analysis of an R2* map of healthy human brain that is automatic, reproducible, and realizable in a single examination on a 3T MR imager. Such a tool could be useful to measure iron accumulation in neurodegenerative diseases.


METHODS
In all, 18 healthy subjects underwent MR imaging at a field strength of 3T: 1) six consecutive T2*-weighted gradient-echo volumes were acquired using a segmented echo-planar imaging sequence and 2) a conventional dual-echo turbo spin echo sequence was also applied to acquire T2-weighted images. Images were realigned and spatial correction was performed using a template brain dataset with SPM2. For each subject we performed a voxel-by-voxel nonlinear least-squares fitting of the data acquired at the six echo times to obtain a monoexponential signal decay curve. The reproducibility and sensitivity to age variation were assessed by voxel-based analysis.


RESULTS
The reproducibility tests in whole brain analysis showed little R2* variation. Furthermore, the statistical analysis, performed on each brain voxel, revealed a significant positive correlation between age and MR values located in regions where a slow and constant age-related iron deposition is known.


CONCLUSIONS
Our method, combining data acquisition and data processing, demonstrates the feasibility of voxel-based analysis on an R2* map and affords a high degree of sensitivity and good reproducibility while maintaining high spatial resolution.
Péran
Patrice
P
Department of Neuroradiology, IRCCS Foundation Santa Lucia, Italy. p.peran@hsantalucia.it
Hagberg
Gisela
G
Luccichenti
Giacomo
G
Cherubini
Andrea
A
Brainovich
Valentina
V
Celsis
Pierre
P
Caltagirone
Carlo
C
Sabatini
Umberto
U
D000328:Adult; D016477:Artifacts; D001931:Brain Mapping / Q000379:methods*; D005260:Female; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D016018:Least-Squares Analysis; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity