Cytostatic drugs differentially affect phenotypic features of porcine coronary artery smooth muscle cell populations.
We studied the effects of cytostatic drugs on porcine coronary artery spindle-shaped (S) and rhomboid (R) smooth muscle cell (SMC) biological activities related to intimal thickening (IT) formation. Imatinib, and to a lesser extent curcumin, decreased proliferation of S- and R-SMCs and migratory and urokinase activities of R-SMCs more efficiently compared with cyclosporine plus rapamycin. Imatinib increased the expression of alpha-smooth muscle actin in both SMC populations and that of smoothelin in S-SMCs. It decreased S100A4 expression in R-SMCs. By promoting SMC quiescence and differentiation imatinib and curcumin may represent valid candidates for restenosis preventive and therapeutic strategies.
Prunotto
Marco
M
Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, Rue Michel Servet 1, 1211 Geneva 4, Switzerland.
Bacchetta
Marc
M
Jayaraman
Swaminathan
S
Galloni
Marco
M
Van Eys
Guillaume
G
Gabbiani
Giulio
G
Bochaton-Piallat
Marie-Luce
ML
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003331:Coronary Vessels / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D054697:Cytostatic Agents / Q000494:pharmacology*; D015151:Immunoblotting; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D010641:Phenotype; D009418:S100 Proteins / Q000378:metabolism; D013552:Swine; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism
D015415:Biomarkers; D054697:Cytostatic Agents; D009418:S100 Proteins; D014568:Urokinase-Type Plasminogen Activator