Expression profiling identifies novel Hh/Gli-regulated genes in developing zebrafish embryos.
The Hedgehog (Hh) signaling pathway plays critical instructional roles during embryonic development. Misregulation of Hh/Gli signaling is a major causative factor in human congenital disorders and in a variety of cancers. The zebrafish is a powerful genetic model for the study of Hh signaling during embryogenesis, as a large number of mutants that affect different components of the Hh/Gli signaling system have been identified. By performing global profiling of gene expression in different Hh/Gli gain- and loss-of-function scenarios we identified known (e.g., ptc1 and nkx2.2a) and novel Hh-regulated genes that are differentially expressed in embryos with altered Hh/Gli signaling function. By uncovering changes in tissue-specific gene expression, we revealed new embryological processes that are influenced by Hh signaling. We thus provide a comprehensive survey of Hh/Gli-regulated genes during embryogenesis and we identify new Hh-regulated genes that may be targets of misregulation during tumorigenesis.
Department of Biology, University of Massachusetts, Amherst, MA 01003-9297, USA.
Sadie A
SA
Bergeron
Luis A
LA
Milla
Rosario
R
Villegas
Meng-Chieh
MC
Shen
Shawn M
SM
Burgess
Miguel L
ML
Allende
Rolf O
RO
Karlstrom
Verónica
V
Palma
D000818:Animals; D004625:Embryo, Nonmammalian; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins / Q000235:genetics*; D009369:Neoplasms / Q000209:etiology / Q000235:genetics; D015513:Oncogene Proteins / Q000235:genetics*; D015398:Signal Transduction; D014018:Tissue Distribution; D015534:Trans-Activators / Q000235:genetics*; D015027:Zebrafish; D000071676:Zinc Finger Protein GLI1
D053823:Hedgehog Proteins; D015513:Oncogene Proteins; D015534:Trans-Activators; D000071676:Zinc Finger Protein GLI1