[Study on variation of actinoside C in leaves of Actinidia kolomikta with different growth periods by RP-HPLC].
OBJECTIVE
To determine actinoside C in the leaves of Actinidia kolomikta with different growth periods.


METHODS
The separation was performed at 25 degrees C on ZORBAX Extend C18 column (4.6 mm x 250 mm, 5 microm), using amixture of methanol and water (51:49) as a mobile phase. The flow rate was 1.2 mL x min(-1), and the wavelength for measurement was 267 nm.


RESULTS
The results showed that the contents of actinoside C in the leaves of A. kolomikta were variety in different growth periods. Actinoside C could reach its highest content in the middle ten days of June, then the content would decrease in the middle ten days of July slightly, it could reach their lowest content in the middle ten days of August.


CONCLUSIONS
The optimal collective date for A. kolomikta are in the middle ten days of June.
Chemistry College of Jilin University, Changchun 130012, China. jinyr@jlu.edu.cn
Yong-Ri
YR
Jin
Ming-Yu
MY
Gui
Juan
J
Lu
Xu-Wen
XW
Li
Ji-Qing
JQ
Xu
D029042:Actinidia / Q000737:chemistry* / Q000254:growth & development; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D047309:Flavones / Q000032:analysis* / Q000737:chemistry; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry* / Q000254:growth & development; D010946:Plants, Medicinal / Q000737:chemistry / Q000254:growth & development; D012621:Seasons
D047309:Flavones; D006027:Glycosides; C532968:actinoside C