Predicting cell-penetrating peptides.
Possibility to predict short peptide sequences capable to penetrate the plasma membrane opens new opportunities for developing peptide based intracellular delivery vectors, called cell-penetrating peptides (CPPs). Predictions of CPPs, however are often based on trial and error and may not always lead to new potent sequences. In this review we discuss different problems associated with CPP prediction. Additionally, the used methods of CPP prediction are compared. Also, a few suggestions are made for designing new CPP sequences and improvement of predictions.
Hansen
Mats
M
Department of Neurochemistry, Stockholm University 10691, Stockholm, Sweden. mats@neurochem.su.se
Kilk
Kalle
K
Langel
Ulo
U
D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D016503:Drug Delivery Systems / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics
D002352:Carrier Proteins; D010455:Peptides