Multiple regulatory genes in the salinomycin biosynthetic gene cluster of Streptomyces albus CCM 4719.
A DNA fragment containing part of the salinomycin biosynthetic gene cluster from industrial strain Streptomyces albus CCM 4719 was cloned. Sequence analysis of the 25.809-kbp fragment revealed the presence of 8 open reading frames (ORFs), including two large ORFs encoding three modular sets of oligoketide synthase, followed by three genes (salRI, salRII, salRIII) encoding transcriptional regulators. The first two regulators, SalRI and SalRII, belonged to the novel LAL family of large transcriptional regulators. SalRIII was highly similar to the NysRIV, AmphRIV, and FscRI transcriptional regulators from the oligoene macrolides nystatin, amphotericin, and R008/candicidin clusters, respectively.
Institute of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovakia.
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R
Knirschová
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Nováková
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L
Fecková
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J
Timko
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J
Turna
J
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Bistáková
J
J
Kormanec
D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015723:Gene Library; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011714:Pyrans / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D000095662:Polyether Polyketides
D004269:DNA, Bacterial; D048630:Polyketide Synthases; D011714:Pyrans; C010327:salinomycin; D000095662:Polyether Polyketides