A novel ferritin gene, SferH-5, reveals heterogeneity of the 26.5-kDa subunit of soybean (Glycine max) seed ferritin.
A novel ferritin cDNA, SferH-5, has been cloned from 7-day-old soybean seedlings. Putative SferH-5 has 96% identity with SferH-1 reported previously. All the five amino acid variants distributed in the mature region are not involved in highly conserved residues associated with ferroxidase activity center. We speculate that SferH-5 encodes a novel 26.5-kDa subunit of soybean seed ferritin, which is designated H-5 in this study. Recombinant H-5 was able to assemble, together with co-expressed H-2, as a functional soybean seed ferritin-like complex, H-5/H-2. Our data reveal the potential heterogeneity of the 26.5-kDa subunit of soybean seed ferritin.
Dong
Xiangbai
X
The State Key Laboratory of Pharmaceutical, Biotechnology and Department of Biochemistry, College of Life Sciences, Nanjing University, Nanjing 210093, PR China.
Sun
Qiming
Q
Wei
Dongping
D
Li
Jiahuang
J
Li
Jie
J
Tang
Bo
B
Jia
Qi
Q
Hu
Wenjun
W
Zhao
Yongjuan
Y
Hua
Zi-Chun
ZC
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005293:Ferritins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D014644:Genetic Variation; D005822:Genetic Vectors; D007501:Iron / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000196:embryology / Q000235:genetics*; D016415:Sequence Alignment; D013025:Glycine max / Q000235:genetics*; D014158:Transcription, Genetic
D010940:Plant Proteins; D021122:Protein Subunits; D011994:Recombinant Proteins; D005293:Ferritins; D007501:Iron