Neural complex-specific expression of xylosyl N-glycan in Ciona intestinalis.
We herein report N-glycosylation profiles of the individual tissues derived from the ascidian Ciona intestinalis. Multidimensional HPLC mapping revealed that the C. intestinalis expresses high-mannose-type oligosaccharides as major N-glycans, along with paucimannose-type and complex-type oligosaccharides, in a tissue-specific manner. Notably, the trimannosyl core carrying beta1,2-xylose and alpha1,3-fucose residues was identified as a principal N-glycan in the neural complex. As far as we know, this is the first description of xylosyl N-glycan expressed in deuterostome. Furthermore, we found that this xylosyl N-glycan is exclusively displayed on a membrane-associated protein so far described as a putative protein whose gene expression is specific for the neural complex. These data suggested that the xylosyl N-glycan is associated with some neural functions of C. intestinalis.
Yagi
Hirokazu
H
Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan.
Nakagawa
Masashi
M
Takahashi
Noriko
N
Kondo
Sachiko
S
Matsubara
Mamoru
M
Kato
Koichi
K
D000595:Amino Acid Sequence; D000818:Animals; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002938:Ciona intestinalis / Q000196:embryology / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification; D006031:Glycosylation; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009420:Nervous System / Q000196:embryology / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
D006023:Glycoproteins; D011134:Polysaccharides; D014994:Xylose