Amentoflavone, a biflavonoid from Biophytum sensitivum augments lymphocyte proliferation, natural killer cell and antibody dependent cellular cytotoxicity through enhanced production of IL-2 and IFN-gamma and restrains serum sialic acid and gamma glutamyl transpeptidase production in tumor - bearing animals.
Modulation of immune response is highly relevant in tumor cell destruction. The present study is focused on the effect of amentoflavone, a biflavonoid from Biophytum sensitivum on cell-mediated immune responses in normal and tumor-bearing control animals. Tumor was induced in BALB/c mice by intraperitoneal injection of Ehrlich ascites carcinoma cells. Treatment of amentoflavone significantly enhanced natural killer cell activity in normal (42.8% cell lysis) and tumor bearing animals (48.2% cell lysis) on the fifth day, which was much earlier compared to tumor-bearing control animals (20.2% cell lysis on day 9). Antibody-dependent cellular cytotoxicity was also increased in amentoflavone -treated normal (41% cell lysis on day 9) and tumor bearing animals (43.8% cell lysis on day 9) compared to untreated tumor bearing control animals (maximum of 15.2% cell lysis on day 13). Amentoflavone administration could significantly enhance the mitogen-induced splenocyte, thymocyte, and bone marrow cell proliferation. Treatment of amentoflavone significantly elevated the production of interleukin-2 and interferon-gamma in normal and Ehrlich ascites carcinoma-bearing animals. Moreover amentoflavone treatment significantly reduced the elevated levels of serum sialic acid and serum gamma glutamyl transpeptidase activity in tumor bearing animals.
Department of Immunology, Amala Cancer Research Center, Amala Nagar, Thrissur 680 555, Kerala State, India.
Chandrasekharan
C
Guruvayoorappan
Girija
G
Kuttan
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects*; D044946:Biflavonoids / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019158:N-Acetylneuraminic Acid / Q000097:blood*; D005723:gamma-Glutamyltransferase / Q000096:biosynthesis / Q000097:blood*
D044946:Biflavonoids; D007376:Interleukin-2; D007371:Interferon-gamma; C011164:amentoflavone; D005723:gamma-Glutamyltransferase; D019158:N-Acetylneuraminic Acid