Interacting proteins Rtt109 and Vps75 affect the efficiency of non-homologous end-joining in Saccharomyces cerevisiae.
One of the key pathways for DNA double-stranded break (DSB) repair is the non-homologous end-joining (NHEJ) pathway, which directly re-ligates two broken ends of DNA. Using a plasmid repair assay screen, we identified that the deletion strain for RTT109 had a reduced efficiency for NHEJ in yeast. This deletion strain also had a reduced efficiency to repair induced chromosomal DSBs in vivo. Tandem-affinity purification of Rtt109 recovered Vps75 as a physical interacting protein. Deletion of VPS75 was also shown to have an effect on the efficiency of NHEJ in both the plasmid repair and the chromosomal repair assays. In addition, deletion mutants for both RTT109 and VPS75 showed hypersensitivity to different DNA damaging agents. Our genetic interaction analysis supports a role for RTT109 in DNA damage repair. We propose that one function of the Rtt109-Vps75 interacting protein pair is to affect the efficiency of NHEJ in yeast. Vps75 but not Rtt109 also seem to have an effect on the efficiency of DSB repair using homologous recombination.
Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ont., Canada K1S 5B6.
Matthew
M
Jessulat
Md
M
Alamgir
Hamid
H
Salsali
Jack
J
Greenblatt
Jianhua
J
Xu
Ashkan
A
Golshani
D002875:Chromosomes / Q000648:ultrastructure; D004249:DNA Damage; D004260:DNA Repair; D019281:Dimerization; D017353:Gene Deletion; D051548:Histone Acetyltransferases / Q000235:genetics* / Q000502:physiology*; D008957:Models, Genetic; D018832:Molecular Chaperones / Q000235:genetics / Q000502:physiology*; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000502:physiology*
D018832:Molecular Chaperones; D029701:Saccharomyces cerevisiae Proteins; C525548:Vps75 protein, S cerevisiae; D051548:Histone Acetyltransferases; C517120:Rtt109 protein, S cerevisiae