[Effects of tetrandrine on Ang II-induced cardiomyocyte hypertrophy and p-ERK1/2 expression].
OBJECTIVE
To observe effects of tetrandrine (Tet) on angiotensin II (Ang II)-induced cardiomyocyte hypertrophy and the activity and expression of phosphorylated ERK1/2 (p-ERK1/2).


METHODS
In the primary culture of neonatal rat cardiomyocytes, as indexes of cardiomyocyte hypertrophy, pulsation rate was measured under phase contrast microscope. Cell size was determined by cell morphology analytical system. The total protein was determined by coomassie brilliant blue and protein synthesis rate was measured by [3H]-Leucine incorporation. ERK activity was measured by immuno-precipitation. The expression of p-ERK1/2 was assessed using Western blot.


RESULTS
Tet can decrease Ang II-induced elevations of the pulsation rate, cell size, total protein and protein synthesis rate; inhibit the activity and expression of p-ERK1/2.


CONCLUSIONS
The anti-hypertrophic effect of Tet on Ang II-induced cardiomyocyte hypertrophy was associated with inhibition of ERK1/2 signaling pathway.
Department of Emergency Medicine, Tongji Hospital of Huazhong University of Science and Technology, Wuhan 430030, China. zdx9599@sina.com
Dai-Xing
DX
Zhou
Guang-Tian
GT
Yang
Xue-Xin
XX
He
Qiang
Q
Liu
D000804:Angiotensin II / Q000633:toxicity; D000818:Animals; D000831:Animals, Newborn; D044182:Benzylisoquinolines / Q000302:isolation & purification / Q000494:pharmacology*; D015153:Blotting, Western; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology; D006984:Hypertrophy; D047468:Immunoprecipitation; D008858:Microscopy, Phase-Contrast; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010766:Phosphorylation / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D014176:Protein Biosynthesis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D031609:Stephania tetrandra / Q000737:chemistry
D044182:Benzylisoquinolines; D004365:Drugs, Chinese Herbal; D000804:Angiotensin II; C009438:tetrandrine; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3