Dendritic cell populations in colon and mesenteric lymph nodes of patients with Crohn's disease.
Dendritic cells (DCs) are key cells in innate and adaptive immune responses that determine the pathophysiology of Crohn's disease. Intestinal DCs migrate from the mucosa into mesenteric lymph nodes (MLNs). A number of different markers are described to define the DC populations. In this study we have identified the phenotype and localization of intestinal and MLN DCs in patients with Crohn's disease and non-IBD patients based on these markers. We used immunohistochemistry to demonstrate that all markers (S-100, CD83, DC-SIGN, BDCA1-4, and CD1a) showed a different staining pattern varying from localization in T-cell areas of lymph follicles around blood vessels or single cells in the lamina propria and in the MLN in the medullary cords and in the subcapsular sinuses around blood vessels and in the T-cell areas. In conclusion, all different DC markers give variable staining patterns so there is no marker for the DC.
Centre for Experimental and Molecular Medicine, Academic Medical Centre, Amsterdam, The Netherlands. m.i.verstege@amc.uva.nl
Marleen I
MI
Verstege
Fiebo J W
FJ
ten Kate
Susanne M
SM
Reinartz
Cornelis M
CM
van Drunen
Frederik J M
FJ
Slors
Willem A
WA
Bemelman
Florry A
FA
Vyth-Dreese
Anje A
AA
te Velde
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000378:metabolism; D018949:Antigens, CD1 / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D003106:Colon / Q000378:metabolism / Q000473:pathology*; D003424:Crohn Disease / Q000378:metabolism / Q000473:pathology*; D003713:Dendritic Cells / Q000378:metabolism / Q000473:pathology*; D006023:Glycoproteins; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology*; D037181:Lectins, C-Type / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D008643:Mesentery; D008875:Middle Aged; D011956:Receptors, Cell Surface / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism; D018180:Thrombomodulin; D000097802:CD83 Antigen; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D015703:Antigens, CD; D018949:Antigens, CD1; D000954:Antigens, Surface; D015415:Biomarkers; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D007136:Immunoglobulins; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D009418:S100 Proteins; D018180:Thrombomodulin; D000097802:CD83 Antigen; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C437944:CD1C protein, human; C419393:CD1a antigen; C546931:THBD protein, human