Evaluation of a bench-top coagulation analyzer for measurement of prothrombin time, activated partial thromboplastin time, and fibrinogen concentrations in healthy dogs.
OBJECTIVE
To evaluate a bench-top coagulation analyzer for determination of prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen concentration in healthy dogs.


METHODS
55 healthy adult dogs.


METHODS
PT, APTT, and fibrinogen concentration were determined by use of the coagulation analyzer. Values were compared with results obtained independently by a conventional laboratory.


RESULTS
Correlations (with 95% confidence intervals) between the coagulation analyzer and conventional laboratory values were 0.760 (0.610 to 0.857), 0.700 (0.448 to 0.721), and 0.896 (0.878 to 0.918) for PT, APTT, and fibrinogen concentration, respectively. Using linear regression, comparison of data from the coagulation analyzer and the conventional laboratory provided equations relating the coagulation analyzer values with values from the conventional laboratory and suggested that APTT and fibrinogen values from the coagulation analyzer and conventional laboratory were approximately the same within expected random variation. Prothrombin time values for the coagulation analyzer were significantly offset from the PT values for the conventional laboratory but still were correlated reasonably well with the conventional laboratory values.


CONCLUSIONS
By use of the mechanical method of analysis, fibrinogen concentrations obtained with a bench-top coagulation analyzer correlated well with results for a conventional laboratory, indicating that the coagulation analyzer is a reliable instrument for determination of this coagulation variable. Coagulation analyzer results for PT and APTT correlated less strongly with those for the conventional laboratory, but they would still be considered clinically reliable.
Mineo
Heather K
HK
Advanced Critical Care and Internal Medicine, 3021 Edinger Avenue, Tustin, CA 92780, USA.
Garabed
Rebecca B
RB
D000818:Animals; D004285:Dogs / Q000097:blood* / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism*; D006262:Health; D010314:Partial Thromboplastin Time / Q000295:instrumentation / Q000662:veterinary*; D019095:Point-of-Care Systems; D011517:Prothrombin Time / Q000295:instrumentation / Q000662:veterinary*; D012016:Reference Values; D012680:Sensitivity and Specificity
D005340:Fibrinogen