Quantitative comparison between microfluidic and microtiter plate formats for cell-based assays.
In this paper, we compare a quantitative cell-based assay measuring the intracellular Ca2+ response to the agonist uridine 5'-triphosphate in Chinese hamster ovary cells, in both microfluidic and microtiter formats. The study demonstrates that, under appropriate hydrodynamic conditions, there is an excellent agreement between traditional well-plate assays and those obtained on-chip for both suspended immobilized cells and cultured adherent cells. We also demonstrate that the on-chip assay, using adherent cells, provides the possibility of faster screening protocols with the potential for resolving subcellular information about local Ca2+ flux.
Department of Electronics, University of Glasgow, Glasgow, G12 8LT, UK.
Huabing
H
Yin
Nicola
N
Pattrick
Xunli
X
Zhang
Norbert
N
Klauke
Hayley C
HC
Cordingley
Steven J
SJ
Haswell
Jonathan M
JM
Cooper
D000818:Animals; D016466:CHO Cells; D002118:Calcium / Q000032:analysis / Q000737:chemistry; D002413:Cations, Divalent / Q000032:analysis / Q000737:chemistry; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D006224:Cricetinae; D003412:Cricetulus; D008024:Ligands; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D015398:Signal Transduction; D014544:Uridine Triphosphate / Q000378:metabolism
D002413:Cations, Divalent; D008024:Ligands; D002118:Calcium; D014544:Uridine Triphosphate