Characterization of monomeric and oligomeric flavan-3-ols from barley and malt by liquid chromatography-ultraviolet detection-electrospray ionization mass spectrometry.
The pattern of the monomeric and oligomeric flavan-3-ols for 10 barley varieties and the corresponding malts were identified and quantified using high-performance liquid chromatography-ultraviolet detection-electrospray ion trap mass spectrometry. The Folin-Ciocalteau and the vanillin spectrophotometric assays were used for the assessment of the total polyphenol and total flavan-3-ol content, respectively, and the antioxidant activity was determined as the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and the ferricyanide reducing power. Catechin and prodelphinidin B3 were respectively the major monomeric and dimeric flavan-3-ols. Moreover, prodelphinidin B3 was shown to be the main contributor for the radical scavenging activity both for barley and malt.
Dvorakova
Marketa
M
Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology, Technicka 5, 166 28 Prague, Czech Republic. marketa.dvorakova@vscht.cz
Moreira
Manuela M
MM
Dostalek
Pavel
P
Skulilova
Zuzana
Z
Guido
Luís F
LF
Barros
Aquiles A
AA
D000872:Anthocyanins / Q000032:analysis; D000975:Antioxidants / Q000032:analysis; D002392:Catechin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D001467:Hordeum / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000872:Anthocyanins; D000975:Antioxidants; D005419:Flavonoids; C472180:prodelphinidin B-2 3'-O-gallate; C404987:flavan-3-ol; D002392:Catechin