Beta-catenin defines head versus tail identity during planarian regeneration and homeostasis.
After amputation, freshwater planarians properly regenerate a head or tail from the resulting anterior or posterior wound. The mechanisms that differentiate anterior from posterior and direct the replacement of the appropriate missing body parts are unknown. We found that in the planarian Schmidtea mediterranea, RNA interference (RNAi) of beta-catenin or dishevelled causes the inappropriate regeneration of a head instead of a tail at posterior amputations. Conversely, RNAi of the beta-catenin antagonist adenomatous polyposis coli results in the regeneration of a tail at anterior wounds. In addition, the silencing of beta-catenin is sufficient to transform the tail of uncut adult animals into a head. We suggest that beta-catenin functions as a molecular switch to specify and maintain anteroposterior identity during regeneration and homeostasis in planarians.
Gurley
Kyle A
KA
Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, 401 MREB, 20N 1900E, Salt Lake City, UT 84132, USA. sanchez@neuro.utah.edu
Rink
Jochen C
JC
Sánchez Alvarado
Alejandro
A
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D025601:Adenomatous Polyposis Coli Protein / Q000737:chemistry / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D019521:Body Patterning; D000072261:Dishevelled Proteins; D020869:Gene Expression Profiling; D017491:Genes, APC; D006257:Head; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D006706:Homeostasis; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D010932:Planarians / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference; D012038:Regeneration; D015398:Signal Transduction; D013623:Tail; D051176:beta Catenin / Q000737:chemistry / Q000235:genetics / Q000502:physiology*
D048868:Adaptor Proteins, Signal Transducing; D025601:Adenomatous Polyposis Coli Protein; D000072261:Dishevelled Proteins; D015801:Helminth Proteins; D010750:Phosphoproteins; D051176:beta Catenin