Dissecting protein structure and function using directed evolution.
To characterize the contributions of individual amino acids to the structure or function of a protein, researchers have adopted directed evolution approaches, which use iterated cycles of mutagenesis and selection or screening to search vast areas of sequence space for sets of mutations that provide insights into the protein of interest.
Yuen
Courtney M
CM
Howard Hughes Medical Institute and Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Liu
David R
DR
D019020:Directed Molecular Evolution / Q000379:methods / Q000639:trends*; D019143:Evolution, Molecular; D016297:Mutagenesis, Site-Directed / Q000379:methods / Q000639:trends*; D015202:Protein Engineering / Q000379:methods / Q000639:trends*; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D013329:Structure-Activity Relationship
D011506:Proteins