Antitumor effect of soluble beta-1,3-glucan from Agrobacterium sp. R259 KCTC 1019.
Beta-1,3-glucans enhance immune reactions such as antitumor, antibacterial, antiviral, anticoagulatory, and wound healing activities. beta-1,3-Glucans have various functions depending on the molecular weight, degree of branching, conformation, water solubility, and intermolecular association. The molecular weight of the soluble glucan was about 15,000 as determined by a high-performance size exclusion chromatography. From the infrared (IR) and 13C NMR analytical data, the purified soluble glucan was found to exclusively consist of beta-D-glucopyranose with 1,3 linkage. We tested the immunestimulating activities of the soluble beta-1,3-glucan extracted from Agrobacterium sp. R259 KCTC 1019 and confirmed the following activities. IFN-gamma and each cytokines were induced in the spleens and thymus of mice treated with soluble beta-1,3-glucan. Adjuvant effect was observed on antibody production. Nitric oxide was synthesized in monocytic cell lines treated with beta-1,3-glucan. The cytotoxic and antitumor effects were observed on various cancer cell lines and ICR mice. These results strongly suggested that this soluble beta-1,3-glucan could be a good candidate for an immune-modulating agent.
Shim
Jung-Hyun
JH
Department of Bioscience and Biotechnology, Konkuk University, Seoul 143- 701, Korea.
Sung
Ki-Joong
KJ
Cho
Min-Chul
MC
Choi
Won-A
WA
Yang
Young
Y
Lim
Jong-Seok
JS
Yoon
Do-Young
DY
D000818:Animals; D000970:Antineoplastic Agents / Q000276:immunology / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004195:Disease Models, Animal; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008813:Mice, Inbred ICR; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D012231:Rhizobium / Q000737:chemistry*; D012995:Solubility; D014867:Water; D047071:beta-Glucans / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*
D000970:Antineoplastic Agents; D047071:beta-Glucans; D014867:Water; C033363:beta-1,3-glucan