A rare case of lung coinfection by Streptomyces cinereoruber and Haemophilus influenzae in a patient with severe chronic obstructive pulmonary disease: characterization at species level using molecular techniques.
Streptomyces cinereoruber isolated from a patient with severe respiratory failure that could not be identified by culture was correctly identified at the species level by comparative sequence analysis of 16S rDNA and the hypervariable alpha-region of 16S rDNA. Restriction fragment length polymorphism, morphologic, and biochemical characteristics have confirmed their identity.
Manteca
Angel
A
Area de Microbiologia, Departamento de Biologia Funcional, Instituto Universitario de Biotecnologia de Asturias (IUBA), Universidad de Oviedo, 33006 Oviedo, Asturias, Spain. mantecaangel@uniovi.es
Pelaez
Ana Isabel
AI
del Mar Garcia-Suarez
Maria
M
Hidalgo
Elisa
E
del Busto
Benigno
B
Mendez
Francisco J
FJ
D000368:Aged; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006192:Haemophilus Infections / Q000150:complications* / Q000188:drug therapy; D006193:Haemophilus influenzae / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D011241:Prednisone / Q000627:therapeutic use; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013902:Radiography, Thoracic; D013302:Streptomyces / Q000235:genetics / Q000302:isolation & purification*; D013420:Sulfamethoxazole / Q000627:therapeutic use; D014057:Tomography, X-Ray Computed; D014295:Trimethoprim / Q000627:therapeutic use
D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D014295:Trimethoprim; D013420:Sulfamethoxazole; D011241:Prednisone