Evaluation of the one-step multiplex real-time reverse transcription-PCR ProFlu-1 assay for detection of influenza A and influenza B viruses and respiratory syncytial viruses in children.
We evaluated the one-step multiplex real-time reverse transcription-PCR ProFlu-1 assay for the detection of influenza A and influenza B viruses and respiratory syncytial viruses from 353 pediatric nasopharyngeal aspirates. As assessed by comparison with the results of immunofluorescence testing and cell culture, the specificity and the sensitivity of the ProFlu-1 assay ranged from 97% to 100%. In addition, the ProFlu-1 assay amplified 9% of samples not detected by conventional methods.
Legoff
Jérôme
J
Laboratoire de Microbiologie, Hôpital Européen Georges Pompidou, and Université Paris Descartes, Paris, France. jerome.le-goff@sls.aphp.fr.
Kara
Rachid
R
Moulin
Florence
F
Si-Mohamed
Ali
A
Krivine
Anne
A
Bélec
Laurent
L
Lebon
Pierre
P
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification*; D009981:Influenza B virus / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis*; D010614:Pharynx / Q000821:virology; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis*; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014776:Virus Cultivation / Q000379:methods