Bottom-up genome assembly using the Bacillus subtilis genome vector.
We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.
Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo 194-8511, Japan. mita2001@sfc.keio.ac.jp
Mitsuhiro
M
Itaya
Kyoko
K
Fujita
Azusa
A
Kuroki
Kenji
K
Tsuge
D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011994:Recombinant Proteins