AtHAP3b plays a crucial role in the regulation of flowering time in Arabidopsis during osmotic stress.
The HAP complex has been found in many eukaryotic organisms. HAP recognizes the CCAAT box present in the promoters of 30% of all eukaryotic genes. The HAP complex consists of three subunits--HAP2, HAP3 and HAP5. In this paper, we report the biological function of the AtHAP3b gene that encodes one of the HAP3 subunits in Arabidopsis. Compared with wild-type plants, hap3b-1 and hap3b-2 mutants exhibited a delayed flowering time under long-day photoperiod conditions. Moreover, the transcription levels of FT were substantially lower in the mutants than in the wild-type plants. These results imply that AtHAP3b may function in the control of flowering time by regulating the expression of FT in Arabidopsis. In a subsequent study, AtHAP3b was found to be induced by osmotic stress. Under osmotic stress conditions, the hap3b-1 and hap3b-2 mutants flowered considerably later than the wild-type plants. These results suggest that the AtHAP3b gene plays more important roles in the control of lowering under osmotic stress in Arabidopsis.
Chen
Nai-Zhi
NZ
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Zhang
Xiu-Qing
XQ
Wei
Peng-Cheng
PC
Chen
Qi-Jun
QJ
Ren
Fei
F
Chen
Jia
J
Wang
Xue-Chen
XC
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D023081:CCAAT-Binding Factor / Q000235:genetics / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D035264:Flowers / Q000254:growth & development / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005816:Genetic Complementation Test; D016254:Mutagenesis, Insertional; D009997:Osmotic Pressure; D010641:Phenotype; D017440:Photoperiod; D030821:Plants, Genetically Modified
D029681:Arabidopsis Proteins; D023081:CCAAT-Binding Factor; D017931:DNA Primers; D004269:DNA, Bacterial; D018744:DNA, Plant; C525376:HAP3b protein, Arabidopsis; C036483:T-DNA