Plasmodium falciparum: sequence analysis of the gene encoding the C-terminus region of the merozoite surface protein-1, a potential malaria vaccine antigen, in Iranian clinical isolates.
C-terminal region of merozoite surface protein-1 of Plasmodium falciparum (PfMSP-1) isolated from different parts of the world revealed sequence variability, however no data exist on sequence heterogeneity of this region from Iran. To address this question, DNA encoding the carboxyl (C)-terminal region of PfMSP-1 was amplified in 144 Iranian P. falciparum clinical isolates, using allele type-specific primers. In this study both MAD20 (88.2%) and K1 (7.6%) types were detected. Sequence analysis of 33 and 92 fragments corresponding to pfmsp-1(42) and pfmsp-1(19) revealed eight (15MAD1-15MAD7 and 15KCH) and five [A1 (E/TSR/L), A2 (Q/KNG/F), A3 (E/KNG/F), A4 (E/TSG/L), and A5 (Q/KNG/L)] distinct haplotypes, respectively. E/TSG/L variant type was the predominant haplotype, and reported only from Thailand and India, but E/KNG/L is widespread in Africa, Asia, and Latin America; but not found among Iranian isolates. In summary, result of this study indicates limited antigenic diversity, and thus support the potential utility of the C-terminal region of PfMSP-1 in designing polyvalent vaccine constructs.
Malaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Pasteur Avenue, PO Box 1316943551, Tehran, Iran.
Akram Abouie
AA
Mehrizi
Sedigheh
S
Zakeri
Ali-Hatef
AH
Salmanian
Mohammad Hossein
MH
Sanati
Navid Dinparast
ND
Djadid
D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000737:chemistry*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D007223:Infant; D007492:Iran; D017780:Malaria Vaccines / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D016778:Malaria, Falciparum / Q000469:parasitology; D008297:Male; D020066:Merozoite Surface Protein 1 / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D008875:Middle Aged; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016415:Sequence Alignment
D016054:DNA, Protozoan; D017780:Malaria Vaccines; D020066:Merozoite Surface Protein 1