Cytogenetic characterization of Rhamdia quelen (Siluriformes, Heptapteridae) from the Bodoquena Plateau, Mato Grosso do Sul, Brazil.
We made a cytogenetic study of the fish Rhamdia quelen collected from the Bodoquena Plateau, an isolated national park region in Mato Grosso do Sul State, Brazil. The diploid number was 2n = 58, with 36 metacentric + 16 submetacentric + 6 subtelocentric chromosomes. We found one to three B chromosomes, which were metacentric and submetacentric and of medium size, showing both intra- and interindividual variation. The nucleolus organizer region (NOR) was located in the terminal region of the short arm of submetacentric pair 20. Staining with CMA3 fluorochrome revealed the NOR location, while there was no evidence of fluorescent staining with DAPI. C banding revealed heterochromatin mainly in the terminal regions of the chromosome arms, including the NOR pair. In addition, metacentric pair 2 showed three heterochromatic blocks in the terminal portions and in the pericentromeric region. The B chromosomes appeared euchromatic. The CB + CMA3 staining combination demonstrated only one chromosome pair with fluorescence, probably the NOR-bearing one, while CB + DAPI gave various fluorescent signals, including metacentric pair 2, indicating that these heterochromatic regions are AT-rich in this population of R. quelen. The R. quelen population in this isolated region of Brazil is chromosomally distinct from that of other populations that have been studied.
Moraes
V P O
VP
Departamento de Biologia Geral, Universidade Estadual de Londrina, CCB, Londrina, PR, Brasil.
Cereali
S S
SS
Froehlich
O
O
Dias
A L
AL
D000818:Animals; D001399:Azure Stains; D001938:Brazil; D002397:Catfishes / Q000235:genetics*; D002871:Chromosome Banding; D002875:Chromosomes / Q000378:metabolism; D020732:Cytogenetic Analysis; D005260:Female; D007621:Karyotyping; D008297:Male; D008677:Metaphase; D009697:Nucleolus Organizer Region
D001399:Azure Stains