Application of a chimeric synthetic peptide in the development of a serologic method for the diagnosis of hepatitis G virus infection.
New putative antigenic peptides corresponding to the N- and C-terminal of the E2 envelope protein of GBV-C/HGV were synthesized using solid-phase chemistry. The antigens were obtained in linear and chimeric forms with the main aim of improving the sensitivity of the enzyme immunoassays. Furthermore, CD and FTIR have been used in conjunction to characterize their conformational changes showing that the chimeric peptide presents a more ordered secondary structure than its parent peptides.
Fernández-Vidal
M
M
Department of Peptide and Protein Chemistry, IIQAB-CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain.
Cubero
M D
MD
Ercilla
G
G
Gómara
M J
MJ
Haro
I
I
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D018178:Flaviviridae Infections / Q000175:diagnosis* / Q000821:virology; D029407:GB virus C / Q000276:immunology*; D006525:Hepatitis, Viral, Human / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology; D017433:Protein Structure, Secondary; D012372:ROC Curve; D012698:Serologic Tests / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D014759:Viral Envelope Proteins / Q000737:chemistry
D000939:Epitopes; D010455:Peptides; D014759:Viral Envelope Proteins