Identification of differentially expressed genes associated with cotton fiber development in a chromosomal substitution line (CS-B22sh).
One of the impediments in the genetic improvement of cotton fiber is the paucity of information about genes associated with fiber development. Availability of chromosome arm substitution line CS-B22sh (chromosome 22 short arm substitution from 3-79 (Gossypium barbadense) into a TM-1 (Gossypium hirsutum) background) provides a novel opportunity to study fiber-associated genes because previous studies revealed this line was associated with some superior fiber quality traits compared to TM-1. We used an integrated approach of suppression subtractive hybridization (SSH), microarray, and real-time reverse transcription-polymerase chain reaction (RT-PCR) technologies to identify the potential genes associated with fiber development. Utilizing mRNAs from 15 days post-anthesis (dpa) fibers, we constructed a SSH cDNA library with chromosome substitution line CS-B22sh as the tester and TM-1 as the driver. The SSH cDNA library was screened using microarrays. Microarray analysis showed that 36 genes were differentially expressed in CS-B22sh 15-dpa fiber compared to TM-1 as confirmed by real time RT-PCR. These genes include two beta-tubulins, an actin, a putative kinesin light chain, a cellulose synthase, glycosyl hydrolase family 3 protein, pyruvate decarboxylase, glycoside hydrolase family 5, GDP-mannose pyrophosphorylase, dynamin-like protein, annexin, and a number of genes involved in signal transduction, and protein, nucleic acid, and lipid metabolisms. To our knowledge, this is the first report on identification of differentially expressed fiber-associated genes in a cotton chromosomal substitution line.
Department of Natural Resources and Environmental Sciences, Alabama A&M University, Normal, AL 35762, USA.
Zhengdao
Z
Wu
Khairy M
KM
Soliman
James J
JJ
Bolton
Sukumar
S
Saha
Johnie N
JN
Jenkins
D001692:Biological Transport / Q000235:genetics; D050260:Carbohydrate Metabolism / Q000235:genetics; D032461:Chromosomes, Plant; D032561:Cotton Fiber; D003598:Cytoskeletal Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D015723:Gene Library; D017343:Genes, Plant; D005964:Glucosyltransferases / Q000235:genetics; D003368:Gossypium / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D003598:Cytoskeletal Proteins; D005964:Glucosyltransferases; C478648:cellulose synthase