Polyethylenimine-DNA solid particles for gene delivery.
Polyethylenimine (PEI), a cationic polymer, was used to develop a non-viral vector for gene delivery. A simple, reproducible process is described with which to condense plasmid DNA with PEI. When prepared at the optimum charge ratio of 6.3 ( +/- ; PEI:DNA, 5:1 w/w), PEI-DNA complexes were 30-60 nm in diameter and excluded intercalating dyes from the plasmid DNA. The particles were stable for more than one month at 4 degrees C with respect to size and transfection activity. PEI-condensed DNA transfected a broad range of murine and human tumor cell lines (B16, Lewis Lung, SK-OV-3 and LS180) in vitro in the presence of fetal calf serum. Intraperitoneal administration of PEI-condensed DNA resulted in significant gene expression in a human ovarian cancer peritoneal xenograft model.
Chen
Tao
T
Faculty of Life Sciences, Northwestern Polytechnical University, Shaanxi Xi'an, People's Republic of China. taochen@libang.com.cn
Wang
Zhao
Z
Wang
Rutao
R
Lu
Tingli
T
Wang
Weijiao
W
D000818:Animals; D045744:Cell Line, Tumor; D004247:DNA / Q000008:administration & dosage*; D004587:Electrophoresis, Agar Gel; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D009369:Neoplasms / Q000628:therapy; D011094:Polyethyleneimine / Q000008:administration & dosage*; D014162:Transfection
D011094:Polyethyleneimine; D004247:DNA