Fate mapping using Cited1-CreERT2 mice demonstrates that the cap mesenchyme contains self-renewing progenitor cells and gives rise exclusively to nephronic epithelia.
Classic tissue recombination and in vitro lineage tracing studies suggest that condensed metanephric mesenchyme (MM) gives rise to nephronic epithelium of the adult kidney. However, these studies do not distinguish between cap mesenchyme and pre-tubular aggregates comprising the condensed MM, nor do they establish whether these cells have self-renewing capacity. To address these questions, we generated Cited1-CreER(T2) BAC transgenic mice, which express tamoxifen-regulated Cre recombinase exclusively in the cap mesenchyme. Fate mapping was performed by crossing these mice with the Rosa26R(LacZ) reporter line and evaluating the location and cellular characteristics of LacZ positive cells at different time points following tamoxifen injection. These studies confirmed expected results from previous in vitro analysis of MM cell fate, and provide in vivo evidence that the cap mesenchyme does not contribute to collecting duct epithelium in the adult. Furthermore, by exploiting the temporally regulated Cre recombinase, these studies show that nephronic epithelium arising at different stages of nephrogenesis has distinct spatial distribution in the adult kidney, and demonstrate for the first time that the cap mesenchyme includes a population of self-renewing epithelial progenitor cells.
Boyle
Scott
S
Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232-2372, USA.
Misfeldt
Andrew
A
Chandler
Kelly J
KJ
Deal
Karen K
KK
Southard-Smith
E Michelle
EM
Mortlock
Douglas P
DP
Baldwin
H Scott
HS
de Caestecker
Mark
M
D000818:Animals; D051017:Apoptosis Regulatory Proteins; D019070:Cell Lineage; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000196:embryology*; D008648:Mesoderm / Q000166:cytology*; D051379:Mice; D008822:Mice, Transgenic; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
D051017:Apoptosis Regulatory Proteins; C102208:Cited1 protein, mouse; D009687:Nuclear Proteins; D015534:Trans-Activators