Raceway cultivation of Spirulina platensis using underground water.
The semi-outdoor cultivation of Spirulina platensis was attempted using an underground-water-based medium. Occurrence of contaminant organisms such as Chlorella sp. and Chlamydomonas sp. was not found from a microscopic observation and bacteria were not detected from denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rDNA during the cultivation, owing to pH control and the high quality of the underground water. The mean productivity was high at 10.5 g/m2/d with a range of 4.2-12.3 g/m2/d despite the unfavorable weather conditions of the rainy season. The cultivated S. platensis included a normal protein content of 58.9%. Consequently, the underground water improved the biomass productivity and the biomass quality because of an abundant supplementation of natural minerals and through a contaminant-free culture.
Kim
Choong-Jae
CJ
Environmental Biotechnology Research Center, KRIBB, Daejeon 305-806, Korea.
Jung
Yun-Ho
YH
Ko
So-Ra
SR
Kim
Hong-Ik
HI
Park
Yong-Ha
YH
Oh
Hee-Mock
HM
D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis; D001431:Bacteriological Techniques; D018533:Biomass; D002241:Carbohydrates / Q000032:analysis; D003470:Culture Media / Q000737:chemistry*; D005618:Fresh Water; D008055:Lipids / Q000032:analysis; D008903:Minerals / Q000032:analysis; D053545:Spirulina / Q000737:chemistry / Q000254:growth & development*
D000596:Amino Acids; D001426:Bacterial Proteins; D002241:Carbohydrates; D003470:Culture Media; D008055:Lipids; D008903:Minerals