Osteopontin is an argentophilic protein in the bone matrix and in cells of kidney convoluted tubules.
Nucleolar organising regions (NOR) are part of the nucleolus, containing argyrophilic proteins (nucleoclin/C23, nucleophosmin/B23). They are identified by silver staining at low pH. The method also reveals osteocyte canaliculi and cement lines and granules in the cytoplasm of kidney cells in locations that mimic osteopontin distribution. Human bone and kidney sections, benign and lymphomatous pleural effusions were processed for silver staining to identify AgNOR. Sections were processed in parallel for immunohistochemistry with an antibody direct against osteopontin. In pleural effusions, AgNORs were found increased in the nuclei of lymphoma cells. In bone, Ag staining identified AgNOR in cell nuclei, as well as in osteocyte canaliculi, cement and resting lines. In the distal convoluted tubules of the kidney, silver deposits were also observed in cytoplasmic granules on the apical side of the cells. Immunolocalization of osteopontin closely matched with all these locations in bone and kidney. NOR proteins and osteopontin are proteins containing aspartic acid rich repeats that can bind Ag. Staining protocols using silver nitrate at low pH can identify these proteins on histological sections. AgNOR is a useful histochemical method to identify osteopontin in bone sections.
Inserm, U 922 - LHEA, faculté de medicine, 1, rue haute de reculée, 49045 Angers cedex, France.
F
F
Pascaretti-Grizon
C
C
Gaudin-Audrain
Y
Y
Gallois
N
N
Retaillaud-Gaborit
M-F
MF
Baslé
D
D
Chappard
D034961:Antigens, Nuclear / Q000378:metabolism; D005270:Femur Head / Q000378:metabolism*; D006620:Hip Fractures / Q000378:metabolism; D006622:Hip Prosthesis; D006801:Humans; D007150:Immunohistochemistry; D007672:Kidney Cortex / Q000378:metabolism*; D007684:Kidney Tubules / Q000378:metabolism*; D008223:Lymphoma / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D010003:Osteoarthritis / Q000378:metabolism; D053495:Osteopontin / Q000378:metabolism*; D010996:Pleural Effusion / Q000378:metabolism
D034961:Antigens, Nuclear; D009687:Nuclear Proteins; C051963:nucleolar organizer region associated proteins; D053495:Osteopontin