Germline mutagenesis mediated by Sleeping Beauty transposon system in mice.
Following the descovery of its transposition activity in mammalian culture systems, the Sleeping Beauty (SB) transposon has since been applied to achieve germline mutagenesis in mice. Initially, the transposition efficiency was found to be low in cultured systems, but its activity in germ cells was unexpectedly high. This difference in transposition efficiency was found to be largely dependent on chromosomal status of the host genomic DNA and transposon vector design. The SB transposon system has been found to be suitable for comprehensive mutagenesis in mice. Therefore, it is an effective tool as a forward genetics screen for tagged insertional mutagenesis in mice.
Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Yamadaoka, Suita, Osaka 565-0871, Japan. takeda@mr-envi.med.osaka-u.ac.jp
Junji
J
Takeda
Vincent W
VW
Keng
Kyoji
K
Horie
D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics*; D005822:Genetic Vectors; D005854:Germ Cells / Q000378:metabolism*; D006570:Heterochromatin / Q000378:metabolism; D051379:Mice; D016296:Mutagenesis; D019895:Transposases / Q000378:metabolism*
D004251:DNA Transposable Elements; D006570:Heterochromatin; D019895:Transposases