Glutamine enhances glucose-induced mesangial cell proliferation.
The proliferation of mesangial cells (MC) in the presence of glutamine (0-20 mM) was determined in both low (5 mM) and high (25 mM) glucose-containing medium. Glutamine in a high glucose (HG) environment increased cell proliferation in a dose-dependent manner. Inhibition of glutamine:fructose 6-phosphate amidotransferase (GFAT) and of phosphodiesterase significantly reduced glutamine-induced proliferation. Supraphysiologic levels of glutamine increase MC proliferation in a HG milieu via GFAT and cAMP-dependent pathways, suggesting that glutamine could pose a risk for diabetic nephropathy.
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes 1524, 05508-900 São Paulo, SP, Brazil. lagranha@hotmail.com
Claudia J
CJ
Lagranha
Sonia Q
SQ
Doi
Tania C
TC
Pithon-Curi
Rui
R
Curi
Donald F
DF
Sellitti
D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005947:Glucose / Q000494:pharmacology*; D005973:Glutamine / Q000494:pharmacology*; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / Q000037:antagonists & inhibitors; D006801:Humans; D050527:Mesangial Cells / Q000166:cytology* / Q000187:drug effects*; D010727:Phosphoric Diester Hydrolases / Q000187:drug effects
D005973:Glutamine; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing); D010727:Phosphoric Diester Hydrolases; D005947:Glucose