Structural genomics of protein phosphatases.
The New York SGX Research Center for Structural Genomics (NYSGXRC) of the NIGMS Protein Structure Initiative (PSI) has applied its high-throughput X-ray crystallographic structure determination platform to systematic studies of all human protein phosphatases and protein phosphatases from biomedically-relevant pathogens. To date, the NYSGXRC has determined structures of 21 distinct protein phosphatases: 14 from human, 2 from mouse, 2 from the pathogen Toxoplasma gondii, 1 from Trypanosoma brucei, the parasite responsible for African sleeping sickness, and 2 from the principal mosquito vector of malaria in Africa, Anopheles gambiae. These structures provide insights into both normal and pathophysiologic processes, including transcriptional regulation, regulation of major signaling pathways, neural development, and type 1 diabetes. In conjunction with the contributions of other international structural genomics consortia, these efforts promise to provide an unprecedented database and materials repository for structure-guided experimental and computational discovery of inhibitors for all classes of protein phosphatases.
Albert Einstein College of Medicine, Bronx, NY, USA. almo@aecom.yu.edu
Steven C
SC
Almo
Jeffrey B
JB
Bonanno
J Michael
JM
Sauder
Spencer
S
Emtage
Teresa P
TP
Dilorenzo
Vladimir
V
Malashkevich
Steven R
SR
Wasserman
S
S
Swaminathan
Subramaniam
S
Eswaramoorthy
Rakhi
R
Agarwal
Desigan
D
Kumaran
Mahendra
M
Madegowda
Sugadev
S
Ragumani
Yury
Y
Patskovsky
Johnjeff
J
Alvarado
Udupi A
UA
Ramagopal
Joana
J
Faber-Barata
Mark R
MR
Chance
Andrej
A
Sali
Andras
A
Fiser
Zhong-yin
ZY
Zhang
David S
DS
Lawrence
Stephen K
SK
Burley
D000818:Animals; D018360:Crystallography, X-Ray; D023281:Genomics; D006801:Humans; D005810:Multigene Family; D010749:Phosphoprotein Phosphatases / Q000737:chemistry* / Q000145:classification / Q000235:genetics* / Q000502:physiology; D017422:Sequence Analysis, DNA
D010749:Phosphoprotein Phosphatases