Identification of novel mitochondrial membrane protein (Cdf 3) from Arabidopsis thaliana and its functional analysis in a yeast system.
We screened the Arabidopsis cDNA library to identify functional suppressors of AtBI-1, a gene that suppresses cell death induced by Bax gene expression in yeast. Cdf 3 encodes a 118-amino-acid protein with a molecular mass of 25 kDa. This protein has two uncharacterized domains at amino acids residues 5-64 and 74-117. In the present study, CDF3 was found to induce growth defects in yeast and arrested yeast growth, although the cell-growth defect was somewhat less than that of Bax. Its localization in the inner mitochondria was essential for suppression of yeast-cell proliferation. The morphological abnormality of the intracellular network, which is a hallmark of AtBI-1, was attenuated by Cdf 3 expression.
Kim
Kyung-Min
KM
Department of Environmental Horticulture, Sangju National University, Sangfu 742-711, Korea. kkm@sangju.ac.kr
Jun
Do-Youn
DY
Kim
Sang-Kook
SK
Kim
Chang-Kil
CK
Kim
Byung Oh
BO
Kim
Young-Ho
YH
Park
Wan
W
Sohn
Jae-Keun
JK
Hirata
Aiko
A
Kawai-Yamada
Maki
M
Uchimiya
Hirofumi
H
Kim
Dai-Hee
DH
Sul
Ill-Whan
IW
D000595:Amino Acid Sequence; D017209:Apoptosis; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D015723:Gene Library; D017343:Genes, Plant; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D051336:Mitochondrial Membranes / Q000737:chemistry*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D051028:bcl-2-Associated X Protein / Q000235:genetics
C491862:ATBI-1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D008565:Membrane Proteins; D051028:bcl-2-Associated X Protein