[Construction of novel recombinant strain harboring glycerol dehydratase reactivating factor capable of producing 1,3-propanediol].
The dhaB gene encoding glycerol dehydratase and dhaG dhaF gene encoding glycerol dehydratase reactivating factor from Citrobacterfreundii were amplified by PCR. The temperature control expression vector pHsh harboring yqhD, dhaB, dhaG and dhaF gene was transformed into E. coli JM109 to yield the recombinant strain E. coli JM109 (pHsh-dhaB-dhaG-dhaF-yqhD). The results from SDS-PAGE analysis show that the recombinant product was consistent with the molecular weight predicted from gene sequence. The fermentation result show that the yield of 1,3-propanediol was increased by 28% compared with E. coli JM109(pHsh-dhaB-yqhD).
School of Biotechnology, Southern Yangtze University, The Key Laboratory of Industrial Biotechnology Ministry of Education, Wuxi 214036, China.
Xiao-Mei
XM
Zhang
Jian
J
Zhuge
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D016971:Citrobacter freundii / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D006836:Hydro-Lyases / Q000096:biosynthesis / Q000235:genetics*; D011409:Propylene Glycols / Q000378:metabolism*; D014169:Transformation, Bacterial / Q000235:genetics*
D001426:Bacterial Proteins; D011409:Propylene Glycols; C041787:1,3-propanediol; D006836:Hydro-Lyases; C120061:glycerol dehydratase reactivating factor, Klebsiella pneumoniae; C020671:glycerol dehydratase