Isolation and characterization of two Korean mistletoe lectins.
Two isolectins (KML-IIU and the KML-IIL) were individually isolated from the previously reported Korean mistletoe lectin, KML-C, by using an immunoaffinity column. Molecular weights of the KML-IIU and the KML-IIL were 64 kDa and 60 kDa respectively. Both of the lectins were composed of heterogeneous A and B subunits linked with a disulfide bond, and showed the same carbohydrate-binding specificities for Gal and GalNAc. However, they are different not only in biophysical properties (glycosylation and amino acid compositions) but also bioactivities (cell killing and cytokine induction). The KML-IIL showed 17-145 times stronger in cytotoxicities to various human and mouse cancer cell lines than the KML-IIU. The KML-IIL also induced TNF-alpha secretion from mouse peritoneal macrophages 4.5 times better than the KML-IIU. The results demonstrated isolectins in Korean mistletoe were varied in bioactivities and the KML-IIL may be developed as an anti-cancer agent.
Kang
Tae Bong
TB
School of Food and Biosciences, Handong Global University, Pohang 791-940, Korea.
Song
Seong Kyu
SK
Yoon
Taek Joon
TJ
Yoo
Yung Choon
YC
Lee
Kwan Hee
KH
Her
Erk
E
Kim
Jong Bae
JB
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D006031:Glycosylation; D006801:Humans; D066298:In Vitro Techniques; D007723:Korea; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D014784:Mistletoe / Q000737:chemistry*; D008970:Molecular Weight; D028321:Plant Preparations / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D021122:Protein Subunits; D054788:Ribosome Inactivating Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000596:Amino Acids; D000972:Antineoplastic Agents, Phytogenic; D028321:Plant Preparations; D010940:Plant Proteins; D021122:Protein Subunits; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; D014409:Tumor Necrosis Factor-alpha; C488863:mistletoe lectin II; D054788:Ribosome Inactivating Proteins