[Pathogenicity and genomic sequence comparison of a chicken infectious anemia virus field isolate].
A field strain C14 of chicken infectious anemia virus (CAV) was isolated from a 14 day-old broiler flock with growth runting syndromes. Antibody reactions to inactivated vaccines to avian influenza viruses (AIV) were suppressed in SPF chickens inoculated with C14 strain CAV at 1 day-old. Also C14 strain CAV and reticuloendotheliosis Virus demonstrated a synergism in immunosuppression when chickens were infected with both virus. The viral genomic DNA was amplified by PCR in 3 overlapped fragments and PCR products were cloned into T-vector plasmid for sequencing. The sequencing results indicated that the total genome of C14 strain CAV was 2298bp, it contained 3 overlapped ORF and 1 non-coding regulation fragment. Its whole genome had 97.2% - 99.2% of homogeneity to other several published CAV reference strains. Sequence data indicated that there are many motifs in the non-coding area of about 400bp as the binding sites for transcriptional factors. All these motifs were very conservative. There were some mutations in 3 genes VP1, VP2 and VP3. Relatively, VP1 was less conservative than VP2 and VP3. Among different strains, mutations in these 3 genes were not correlated.
Li
Yan-Peng
YP
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai' an 271000, China. liyanpeng79@163.com
Cui
Zhi-Zhong
ZZ
D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D017727:Chicken anemia virus / Q000235:genetics* / Q000472:pathogenicity*; D002645:Chickens / Q000821:virology*; D016679:Genome, Viral; D017422:Sequence Analysis, DNA
D036022:Capsid Proteins; C442548:VP1 protein, Chicken anemia virus; C442521:VP2 protein, Chicken anemia virus; C091717:VP3 protein, Chicken anemia virus