New developments in the synthesis of oligonucleotide-peptide conjugates.
The stability of oligodeoxynucleotides to trifluoroacetic acid is studied. Pyrimidine oligonucleotides were stable in the conditions used for the removal of t-butyl groups. Oligonucleotide-3'-peptide conjugates carrying pyrimidine oligonucleotides are prepared stepwise using peptide-supports and Fmoc, t-butyl strategy. Using this strategy we have prepared an oligonucleotide-peptide conjugate containing as peptide the leucine-rich fragment of FOS, a transcription factor involved in many important cellular processes. This conjugate has a long peptide sequence with a large number of trifunctional amino acids.
Dipartamento de Química Orgánica, Universidad de Santiago de Compostela, Santiago de Compostela, Spain.
Cecilia
C
Portela
José Luis
JL
Mascareñas
Fernando
F
Albericio
Stefania
S
Mazzini
Clara
C
Caminal
Roger
R
Ramos
Sandra M
SM
Ocampo
Ramon
R
Eritja
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D016350:Leucine Zippers / Q000235:genetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D016760:Proto-Oncogene Proteins c-fos / Q000737:chemistry / Q000235:genetics; D014269:Trifluoroacetic Acid
D009838:Oligodeoxyribonucleotides; D020135:Peptide Nucleic Acids; D016760:Proto-Oncogene Proteins c-fos; D014269:Trifluoroacetic Acid