Visualization of shell matrix proteins in hemocytes and tissues of the Eastern oyster, Crassostrea virginica.
The tissues of the oyster were examined for the presence of shell matrix proteins (SMPs) using a combination of Western, proteomic, and epi-fluorescent microscopy techniques. SMP, including 48 and 55 kDa phosphoproteins, was detected in the epithelial cells of mantle, gill, heart, and adductor muscle and linings of arteries and veins. The 48 kDa SMP circulates continuously within the hemolymph, and is present in the immune system hemocytes. It appears to be secreted from hemocytes on induction of shell repair. We suggest that the 48 and 55 kDa proteins are multifunctional and bridge the process of soft tissue repair and shell formation by mediating cellular activities during immune response as well as interacting with the mineral phase during deposition.
Department of Biological Sciences, Clemson University, Clemson, South Carolina, USA. MBJ@clemson.edu
Mary B
MB
Johnstone
Steve
S
Ellis
Andrew S
AS
Mount
D000818:Animals; D049896:Crassostrea / Q000166:cytology*; D006352:Heart Ventricles / Q000166:cytology; D006434:Hemocytes / Q000166:cytology*; D006651:Histocytochemistry; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D013022:South Carolina
D008565:Membrane Proteins