QTL detection on porcine chromosome 12 for fatty-acid composition and association analyses of the fatty acid synthase, gastric inhibitory polypeptide and acetyl-coenzyme A carboxylase alpha genes.
Refinement of previous QTL on porcine chromosome 12 for fatty-acid composition and a candidate gene association analysis were conducted using an Iberian x Landrace cross. The concentrations of ten fatty acids were assayed in backfat tissue from which four metabolic ratios were calculated for 403 F2 animals. Linkage analysis identified two significant QTL. The first QTL was associated with the average chain length ratio and the percentages of myristic, palmitic and gadoleic acids. The second QTL was associated with percentages of palmitoleic, stearic and vaccenic acids. Based upon its position on SSC12, fatty acid synthase was tested as a candidate gene for the first QTL and no significant effects were found. Similarly, gastric inhibitory polypeptide (GIP) and acetyl-coenzyme A carboxylase alpha (ACACA) were tested as candidate genes for the second QTL using three SNPs in GIP and 15 synonymous SNPs in ACACA cDNA sequences. Two missense SNPs in GIP showed significant effects with palmitoleic and stearic fatty-acid concentration. Highly significant associations were found for two SNPs in ACACA with stearic, palmitoleic and vaccenic fatty-acid concentrations. These associations could be due to linkage disequilibrium with the causal mutations.
Departamento de Mejora Genética Animal, INIA, 28040 Madrid, Spain.
G
G
Muñoz
E
E
Alves
A
A
Fernández
C
C
Ovilo
C
C
Barragán
J
J
Estellé
R
R
Quintanilla
J M
JM
Folch
L
L
Silió
M C
MC
Rodríguez
A I
AI
Fernández
D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D000818:Animals; D033481:Chromosomes, Mammalian; D054890:Fatty Acid Synthase, Type I / Q000235:genetics*; D005227:Fatty Acids / Q000235:genetics* / Q000378:metabolism; D005749:Gastric Inhibitory Polypeptide / Q000235:genetics*; D008040:Genetic Linkage; D020161:Physical Chromosome Mapping; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D013552:Swine / Q000235:genetics* / Q000378:metabolism
D005227:Fatty Acids; D005749:Gastric Inhibitory Polypeptide; D054890:Fatty Acid Synthase, Type I; D000103:Acetyl-CoA Carboxylase