Characterization and applications of reversed-phase column selectivity based on the hydrophobic-subtraction model.
A total of 371 reversed-phase columns have now been characterized in terms of selectivity, based on five solute-column interactions (the hydrophobic-subtraction model). The present study illustrates the use of these data for interpreting peak-tailing and column stability. New insights are also provided concerning column selectivity as a function of ligand and silica type, and the selection of columns for orthogonal separations is re-examined. Some suggestions for the quality control of reversed-phase columns during manufacture are offered.
University of Wisconsin-River Falls, River Falls, WI 54022-5001, USA. daniel.marchand@uwrf.edu
D H
DH
Marchand
L R
LR
Snyder
J W
JW
Dolan
D002853:Chromatography, Liquid / Q000379:methods*; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D008956:Models, Chemical; D012680:Sensitivity and Specificity