Identification of the gene imds-60 in mouse epididymis.
Sperm maturation, including the acquisition of motility and the full ability to fertilize oocyte, occurs during its transit through the dynamic environment of the epididymis. However, the roles of many genes involved in the process of sperm maturation still remain to be found. Based on an expressed sequence tag named imds-60, which was first found in uterus but is highly expressed in epididymis, the full-length cDNA sequence of imds-60 with a complete open reading frame was obtained in mouse epididymis by GenBank searching, polymerase chain reaction-based procedures, and 5'- and 3'-rapid amplification of cDNA ends. This protein was predicted to have an N-terminal signal peptide and a C-terminal DNase I-like domain with nine transmembrane motifs in the middle part of the protein. Northern blot analysis showed that the mRNA of imds-60 was highly expressed in epididymis but at a rather lower level in uterus, seminal vesicle gland, and stomach. Further study revealed that the mRNA of imds-60 is only expressed in corpus and cauda regions of epididymis, not in caput. It is regulated partially by androgen and peaked in male mice aged from 3 weeks to adult. The imds-60 protein might play an important role in cell communication during sperm maturation.
Zhou
Xuan
X
Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031,China.
Wang
Jian
J
Liu
Qiang
Q
Shen
Jia
J
Zheng
Min
M
Zhang
Yonglian
Y
D000818:Animals; D002450:Cell Communication / Q000502:physiology*; D003851:Deoxyribonucleases / Q000235:genetics* / Q000378:metabolism*; D004822:Epididymis / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013091:Spermatogenesis / Q000502:physiology*
C526188:Imds-60 protein, mouse; D008565:Membrane Proteins; D003851:Deoxyribonucleases