Bottom-up genome assembly using the Bacillus subtilis genome vector.
We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.
Itaya
Mitsuhiro
M
Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo 194-8511, Japan. mita2001@sfc.keio.ac.jp
Fujita
Kyoko
K
Kuroki
Azusa
A
Tsuge
Kenji
K
D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011994:Recombinant Proteins