Bacterial adhesion and osteoblast function on titanium with surface-grafted chitosan and immobilized RGD peptide.
Biomaterials-associated infections remain a source of serious complications in modern medicine. When a biomaterial is implanted in the body, the result of successful tissue integration or implant infection depends on the race for the surface between bacteria and tissue cells. One promising strategy to reduce the incidence of infection is the functionalization of the biomaterial surface to inhibit bacterial adhesion and encourage the growth of cells. In this in vitro study, the surface of titanium alloy substrates was first functionalized by covalently grafted chitosan (CS). The cell-adhesive Arg-Gly-Asp (RGD) peptide was then immobilized on the CS-grafted surface through covalent binding of peptide to the free NH(2) groups of CS. Both these functionalized surfaces showed a decrease in adhesion of Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis) compared with the pristine substrate. A significant increase in osteoblast cell attachment, proliferation, and alkaline phosphatase activity was observed on the surface with the immobilized Arg-Gly-Asp peptide. Thus, utilizing surface-grafted chitosan in conjunction with the cell-adhesive peptide to modify the metal surface provides a promising means for enhancing tissue integration of implants by reducing bacterial adhesion and promoting osteoblast functions.
Department of Chemical and Biomolecular Engineering, National University of Singapore, Kent Ridge, Singapore 119260.
Zhilong
Z
Shi
K G
KG
Neoh
E T
ET
Kang
Chyekhoon
C
Poh
W
W
Wang
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D002452:Cell Count; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D013057:Spectrum Analysis; D013211:Staphylococcus aureus / Q000166:cytology* / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000166:cytology* / Q000187:drug effects; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*
D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D048271:Chitosan; D014025:Titanium; D000469:Alkaline Phosphatase