[Production of monoclonal antibodies to Cymbidium mosaic virus and application in orchids virus detection].
Three hybridoma cell lines, 2C6, 5B7 and 12G9, secreting monoclonal antibodies (McAbs) against Cymbidium mosaic virus (CymMV) were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/ C immunized by the CymMV particles. The three McAbs could specifically react with CymMV. The titres of ascitic fluids of two McAbs are up to 10(-6) in I-ELISA. Isotypes and subclasses of the the three McAbs belong to IgG1. Isotypes of light strains of the three McAbs all belong to kappa. They were used in antigen-coated plate (ACP)-ELISA for CymMV detection, and ACP-ELISA could successfully detect 0.487 ng of purified CymMV or virus in plant sap diluted 1:10240. The presence of CymMV in field Orchids tissues was investigated with ACP-ELISA.
Meng
Chun-Mei
CM
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China. mcm1983@126.com
Wu
Jian-Xiang
JX
Xie
Li
L
Zheng
Jin-Kai
JK
Hong
Jian
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008807:Mice, Inbred BALB C; D029595:Orchidaceae / Q000821:virology*; D017863:Potexvirus / Q000276:immunology* / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral