Continuous production of lactosucrose by immobilized Sterigmatomyces elviae mutant.
In this study, in order to develop a continuous production process of lactosucrose in a packed-bed reactor, Sterigmatomyces elviae ATCC 18894 was selected and mutated. The mutant strain of S. elviae showed 54.3% higher lactosucrose production than the wild type. Reaction conditions such as temperature, pH, substrate concentration and flow rate were also optimized. Under optimized reaction conditions (50 degrees C, pH 6.0, 25% sucrose and 25% lactose as substrate, flow rate 1.2 ml/min), the maximum concentration of lactosucrose (192 g/l) was obtained. In a packed-bed reactor, continuous production of lactosucrose was performed using S. elviae mutant immobilized in calcium alginate, and about 180 g/l of lactosucrose production was achieved for 48 days.
Lee
Jong Ho
JH
Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Korea.
Lim
Jung Soo
JS
Park
Chulhwan
C
Kang
Seong Woo
SW
Shin
Hyun Yong
HY
Park
Seung Won
SW
Kim
Seung Wook
SW
D000464:Alginates / Q000378:metabolism; D001487:Basidiomycota / Q000235:genetics / Q000378:metabolism*; D019149:Bioreactors; D002118:Calcium / Q000378:metabolism; D018914:Cells, Immobilized / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D013696:Temperature; D014312:Trisaccharides / Q000096:biosynthesis* / Q000737:chemistry
D000464:Alginates; D014312:Trisaccharides; C081752:galactosucrose; D002118:Calcium