Identification of two entomopathogenic bacteria from a nematode pathogenic to the Oriental beetle, Blitopertha orientalis.
A pathogenic nematode, Butlerius sp., was isolated from Oriental beetle, Blitopertha orientalis. The infective juveniles exhibited dose- as well as time-dependent entomopathogenicity on the larvae of B. orientalis. Two bacterial species, Providencia vermicola (KACC 91278) and Flavobacterium sp. (KACC 91279), were isolated from the infective juveniles and identified. P. vermicola outnumbered Flavobacterium sp. in the nematode host, in which the colony density of P. vermicola was found to be 21 times higher than that of Flavobacterium sp. However, when the two bacterial species were cocultured in culture media without the nematode host, they showed similar growth rates. Both bacteria induced significant entomopathogenicity against Spodoptera exigua larvae infesting economically important vegetable crops, where P. vermicola was more potent than Flavobacterium sp.
Department of Bioresource Sciences, College of Natural Sciences, Andong National University, Andong 760-749, Korea.
Young-Keun
YK
Yi
Hae Woong
HW
Park
Sony
S
Shrestha
Jiae
J
Seo
Yong Ook
YO
Kim
Chul Soo
CS
Shin
Yonggyun
Y
Kim
D000818:Animals; D001517:Coleoptera / Q000382:microbiology / Q000469:parasitology*; D005417:Flavobacterium / Q000302:isolation & purification* / Q000472:pathogenicity; D009348:Nematoda / Q000302:isolation & purification / Q000382:microbiology*; D011532:Providencia / Q000302:isolation & purification* / Q000472:pathogenicity; D018411:Spodoptera / Q000382:microbiology; D013559:Symbiosis