[Monitoring of photodynamic therapy of port wine stain by fluorescence spectroscopy].
The blood drug level and the formation of photoproduct were monitored during photodynamic therapy (PDT) of port wine stain (PWS) by fluorescence spectroscopy. The irradiation was implemented by a 532 nm double-frequency Nd:YAG laser, and the collection of fluorescence spectra was completed with the use of spectrograph and ICCD. In the experiment for validation of the system, the fluorescence basis spectra of hematoporphyrin monomethyl ether (HMME)-sensitized mouse normal skin were constructed, and, by least-square fitting, HMME fluorescence (624 nm) could be discriminated from that of photoproduct (652 nm). The fitting of fluorescence spectra measured from PWS patients skin containing PSD-007 was performed with the same basis spectra as those from the mouse skin. The curves of blood drug level of different patients with significant variance, as well as those of formation and bleaching of photoproduct were obtained. Fluorescence spectroscopy monitoring system and fitting method presented here can provide technical means for rigorous quantitative PDT dosimetry method, and the results obtained here will make for the individual scheme of PDT.
Department of Laser Medicine, Chinese PLA General Hospital, Beijing 100853, China.
Lei
L
Wang
Ying
Y
Gu
Xiao-Song
XS
Li
Fan-Guang
FG
Liu
Chang-Qing
CQ
Yu
D000818:Animals; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008991:Monitoring, Physiologic / Q000379:methods*; D010778:Photochemotherapy; D019339:Port-Wine Stain / Q000628:therapy*; D012867:Skin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*