[Cloning and sequence analysis of alpha satellite DNA in Arctic fox].
Genomic DNAs from Arctic fox were cleaved with restriction enzymes. The prominent ethidium-bromide stanined bands were cloned and sequenced after separation of DNA fragments by agarose gel electrophoresis. Forty-two satellite sequences were obtained by sequencing. Sequence analyses revealed that the satellite monomer in length was 737 bp, with an average G+C content of 51.9%. There were 91%-97% homology among the monomers, and the monomer consisted of three subrepeats of approximately 245 bp in length with 51%-54% homology. The satellite DNAs were specific for canidae, and had 74% homology to the dog centromere-associated nucleotide sequences. In order to distinguish from another satellite DNAs of canidae genome, the satellite was designated as alpha satellite DNA.
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China. ysq2004927@163.com
Shou-Qing
SQ
Yan
Wan-Ju
WJ
Zhu
Xue-Mei
XM
Zhang
Bing
B
Li
Jin-Hai
JH
Sun
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004276:DNA, Satellite / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005589:Foxes / Q000235:genetics*; D023281:Genomics; D008297:Male; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004276:DNA, Satellite; D004262:DNA Restriction Enzymes