Evaluation of in vitro virulence characteristics of the genus Pandoraea in lung epithelial cells.
Pandoraea species are emerging opportunistic pathogens capable of causing chronic lung infections in cystic fibrosis patients. This study examined the interactions of 17 Pandoraea isolates from the five identified species (Pandoraea apista, Pandoraea norimbergensis, Pandoraea pulmonicula, Pandoraea sputorum and Pandoraea pnomenusa) plus two Pandoraea genomospecies isolates with lung epithelial cells and their ability to form biofilms in vitro. Only three isolates showed an ability to invade A549 lung epithelial cells, and only one isolate was able to form biofilms. In contrast, all isolates triggered a pronounced pro-inflammatory response, with elevation of both interleukin (IL)-6 (two- to 19-fold) and IL-8 (10- to 50-fold) above that observed for a control strain of Escherichia coli. This property is likely to be a major factor in the pathogenesis of the genus.
Caraher
Emma
E
Department of Applied Science and National Institute of Cellular Biotechnology, Institute of Technology, Tallaght, Dublin 24, Ireland.
Collins
Jonathan
J
Department of Clinical Microbiology, Trinity College, Dublin, Ireland.|Adelaide, Meath and National Children's Hospital, Tallaght, Dublin 24, Ireland.
Herbert
Gillian
G
Department of Applied Science and National Institute of Cellular Biotechnology, Institute of Technology, Tallaght, Dublin 24, Ireland.
Murphy
Philip G
PG
Department of Clinical Microbiology, Trinity College, Dublin, Ireland.|Adelaide, Meath and National Children's Hospital, Tallaght, Dublin 24, Ireland.
Gallagher
Charles G
CG
St Vincent's University Hospital, Dublin 4, Ireland.
Crowe
Mary J
MJ
St Vincent's University Hospital, Dublin 4, Ireland.
Callaghan
Máire
M
Department of Applied Science and National Institute of Cellular Biotechnology, Institute of Technology, Tallaght, Dublin 24, Ireland.
McClean
Siobhán
S
Department of Applied Science and National Institute of Cellular Biotechnology, Institute of Technology, Tallaght, Dublin 24, Ireland.
D020562:Betaproteobacteria / Q000187:drug effects / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology; D018441:Biofilms / Q000254:growth & development*; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology*; D008168:Lung / Q000473:pathology*; D014774:Virulence / Q000235:genetics*