Micro-magnetic resonance imaging of avian embryos.
Chick embryos are useful models for probing developmental mechanisms including those involved in organogenesis. In addition to classic embryological manipulations, it is possible to test the function of molecules and genes while the embryo remains within the egg. Here we define conditions for imaging chick embryo anatomy and for visualising living quail embryos. We focus on the developing limb and describe how different tissues can be imaged using micro-magnetic resonance imaging and this information then synthesised, using a three-dimensional visualisation package, into detailed anatomy. We illustrate the potential for micro-magnetic resonance imaging to analyse phenotypic changes following chick limb manipulation. The work with the living quail embryos lays the foundations for using micro-magnetic resonance imaging as an experimental tool to follow the consequences of such manipulations over time.
Li
Xiaojing
X
Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee, UK.
Liu
Jia
J
Davey
Megan
M
Duce
Suzanne
S
Jaberi
Neema
N
Liu
Gang
G
Davidson
Gemma
G
Tenent
Seaneen
S
Mahood
Ruth
R
Brown
Phoebe
P
Cunningham
Craig
C
Bain
Andrew
A
Beattie
Kevin
K
McDonald
Laura
L
Schmidt
Katy
K
Towers
Matthew
M
Tickle
Cheryll
C
Chudek
Sandy
S
D019412:Anatomy, Cross-Sectional; D000818:Animals; D001717:Birds / Q000196:embryology*; D002642:Chick Embryo; D003287:Contrast Media; D005682:Gadolinium; D021621:Imaging, Three-Dimensional; D018878:Limb Buds / Q000033:anatomy & histology; D008279:Magnetic Resonance Imaging; D009132:Muscles / Q000196:embryology; D011784:Quail; D014921:Wings, Animal / Q000196:embryology
D003287:Contrast Media; D005682:Gadolinium