Evaluation of antimicrobial, antioxidant and wound-healing potentials of Holoptelea integrifolia.
The methanolic extracts of Holoptelea integrifolia (Roxb.) (Urticaceae) leaves (MLE) and stem bark (MSBE) were studied for the wound-healing potential. Since wound healing is severely hampered by microbial infection and reactive oxygen species (ROS), this study was undertaken to evaluate antimicrobial and antioxidant activity apart from wound-healing activity. The antimicrobial property of the Holoptelea was studied against the six bacterial and five fungal strains using the agar well diffusion method and minimum microbicidal concentration and minimum inhibitory concentration were determined for each strain, in which methanolic extract of stem bark (MSBE) has shown bigger zone of inhibition (11.3-20.4 mm) than methanolic extract of leaves (MLE) (9.6-14.9 mm). The anti-oxidant activity was evaluated by DPPH free radical scavenging activity using HPLC method. The IC(50) values obtained for MSBE (TPC: 78.53+/-1.26 mg/g) and MLE (TPC: 57.71+/-1.45 mg/g) were 37.66+/-0.48 and 50.36+/-0.59 microg/well, respectively. In excision wound model, more than 90% wound healing was recorded in treated groups by 14 days of post surgery, where as only 62.99% was observed in the control group. In incision model, higher breaking strengths and higher hydroxyproline content in treated groups suggested higher collagen re-deposition than the control group. Finally, histopathology studies conformed wound-healing activity of Holoptelea integrifolia.
Pharmacology Division, Indian Institute of Chemical Technology, Hyderabad 500007, India.
Boreddy
B
Srinivas Reddy
R
R
Kiran Kumar Reddy
V G M
VG
Naidu
K
K
Madhusudhana
Sachin B
SB
Agwane
Sistla
S
Ramakrishna
Prakash V
PV
Diwan
D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D004195:Disease Models, Animal; D005609:Free Radicals / Q000378:metabolism; D005658:Fungi / Q000187:drug effects; D020128:Inhibitory Concentration 50; D008297:Male; D008826:Microbial Sensitivity Tests; D024301:Plant Bark; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D027003:Urticaceae / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*
D000890:Anti-Infective Agents; D000975:Antioxidants; D005609:Free Radicals; D010936:Plant Extracts; D017382:Reactive Oxygen Species