[Observation on gametophyte development of Cibotium barometz].
OBJECTIVE
To observe the spores germinating process of Cibotium barometz, and understand the growth principle provided for experience for indoor culturing and further research.


METHODS
The spores of C. barometz were cultured both in inorganic medium and in the soil from original habitat, and the whole process of spores germination and the development of gametophytic were observed under microscope.


RESULTS
The spores germinated about 1-2 weeks after being sowed, and the type of germination belonged to Vittaria-type. The prothallial plates formed in 25 days after being sowed, while hairs developed after the formation of the prothallial plate. The gametophyte formed about 40 days after being sowed. But the type of mature prothalli was cordate. The antheridia formed in 60 days after inoculation, while the archegonia developed in 10 days after the formation of antheridia.


CONCLUSIONS
Soil based indoor culturing of C. barometz spores is practical and can be used for cultivation of C. barometz.
Deng
Hong-Ping
HP
College of Life Science, Southwest University, Chongqing 400715, China. Denghp@swu.edu.cn
Liu
Guang-Hua
GH
Wu
Lian
L
Liao
Xiao-Min
XM
Chen
Shui-Mu
SM
Cui
Ya-Qiong
YQ
D003470:Culture Media / Q000494:pharmacology; D029624:Ferns / Q000166:cytology / Q000502:physiology*; D010946:Plants, Medicinal / Q000033:anatomy & histology / Q000166:cytology / Q000502:physiology*; D012987:Soil; D013170:Spores / Q000166:cytology / Q000187:drug effects / Q000254:growth & development
D003470:Culture Media; D012987:Soil