Galactosyl transferases in mycobacterial cell wall synthesis.
Two galactosyl transferases can apparently account for the full biosynthesis of the cell wall galactan of mycobacteria. Evidence is presented based on enzymatic incubations with purified natural and synthetic galactofuranose (Galf) acceptors that the recombinant galactofuranosyl transferase, GlfT1, from Mycobacterium smegmatis, the Mycobacterium tuberculosis Rv3782 ortholog known to be involved in the initial steps of galactan formation, harbors dual beta-(1-->4) and beta-(1-->5) Galf transferase activities and that the product of the enzyme, decaprenyl-P-P-GlcNAc-Rha-Galf-Galf, serves as a direct substrate for full polymerization catalyzed by another bifunctional Galf transferase, GlfT2, the Rv3808c enzyme.
Belánová
Martina
M
Department of Biochemistry, Comenius University, Faculty of Natural Sciences, Bratislava, Slovakia SK-842 15.
Dianisková
Petronela
P
Brennan
Patrick J
PJ
Completo
Gladys C
GC
Rose
Natisha L
NL
Lowary
Todd L
TL
Mikusová
Katarína
K
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism*; D005685:Galactans / Q000737:chemistry / Q000378:metabolism*; D005700:Galactosyltransferases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D020102:Mycobacterium smegmatis / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D005685:Galactans; D011994:Recombinant Proteins; D005700:Galactosyltransferases