High performance thin layer chromatographic analysis of neutral lipids in the urine of BALB/c mice infected with Echinostoma caproni.
Silica gel high performance thin layer chromatography-densitometry was used to determine the neutral lipid profile in the urine of BALB/c mice experimentally infected with adults of Echinostoma caproni. An approximate 30 worm burden per host induced certain changes in the neutral lipid profile of the mice at 2 to 10 weeks post-infection (PI) relative to the uninfected controls. Infection caused a significant increase (Student's t test, P < 0.05) in the methyl oleate fraction at 6 and 7 weeks PI relative to the uninfected controls. Neutral lipid profiles of urine may serve as an indicator of experimental infection of echinostomes in mice.
Vasta
James D
JD
Department of Chemistry, Lafayette College, Easton, PA, USA.
Fried
Bernard
B
Sherma
Joseph
J
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004449:Echinostoma / Q000472:pathogenicity*; D004451:Echinostomiasis / Q000469:parasitology* / Q000652:urine; D005260:Female; D008055:Lipids / Q000032:analysis* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D009829:Oleic Acids / Q000032:analysis; D014556:Urine / Q000737:chemistry*
D008055:Lipids; D009829:Oleic Acids; C005576:methyl oleate