Effect of anti-KDR antibody on the proliferation of hemangioma vascular endothelial cells in vitro.
The suppressive effect of anti-KDR antibody against VEGF on proliferation of hemangioma-derived vascular endothelial cells (HVECs) was investigated. HVECs from one case of hemangioma in proliferative phase were cultured. Both primary culture and sub-culture were conducted in M199 medium. The HVECs of passage 3 were divided into 4 groups based on the concentrations of anti-KDR antibody. Cell count was performed and inhibitory rate of HVECs was measured before and 9 days after interference. The results showed that the number of HVECs in the anti-KDR antibody-treated groups was significantly decreased and the inhibitory rate of HVECs by anti-KDR antibody (50, 10 and 2 microg/mL) was 84%, 63% and 39% respectively at 9th day after interference, with the difference being significant. In the control group, the number of HVECs was increased significantly. In was concluded that the anti-KDR antibody could suppress the activity of VEGF through blocking the KDR, indicating the potential clinical applications of anti-KDR antibody in the treatment of hemangioma.
Department of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. qzg599@126.com
Yiqing
Y
Li
Yin
Y
Xia
Bi
B
Jin
D000906:Antibodies / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004730:Endothelium, Vascular / Q000473:pathology*; D006391:Hemangioma / Q000473:pathology*; D006801:Humans; D014407:Tumor Cells, Cultured; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000276:immunology*
D000906:Antibodies; D040301:Vascular Endothelial Growth Factor Receptor-2