Fluorimetric study of interaction of merbromin with trypsin.
Interaction of merbromin with trypsin is of bovine origin has been studied by monitoring the absorption steady-state and time-resolved fluorescence spectral properties of the dye. Studies have been done in media of varying pH at different trypsin concentrations. It has been observed that trypsin brings about a quenching of fluorescence of the dye. The quenching is static in nature and the equilibrium constant of dye-trypsin interaction in the ground-state has been determined from quenching studies. Steady-state anisotropy of the dye increases in presence of trypsin in the medium. Values of micro-viscosity in the vicinity of the fluorophore in media containing trypsin have been determined from measurements of fluorescence anisotropy. Time-resolved fluorescence studies indicate the existence of two decaying states for the dye. The fractional contribution to the time-resolved decay changes with pH. The average lifetime, however, does not depend on the concentration of trypsin.
Banerjee
Debashis
D
Department of Chemistry, Durgapur Government College, West Bengal 713214, India.
Das
Parimal Kumar
PK
Singha
Haraprasad
H
Bagchi
Sanjib
S
D016880:Anisotropy; D008622:Merbromin / Q000737:chemistry*; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence; D014357:Trypsin / Q000737:chemistry* / Q000378:metabolism
D014357:Trypsin; D008622:Merbromin