Synergistic protective effect of picroside II and NGF on PC12 cells against oxidative stress induced by H2O2.
Epidemiological studies suggest that nerve growth factor (NGF) is associated with a reduced risk of acute or chronic neuropathies. We studied the synergistic protective effect of picroside II and NGF against the oxidative stress in PC12 cells induced by hydrogen peroxide (H2O2). The fluorescent probe CDCFH was used to assess the intracellular reactive oxygen species (ROS) level, and MTT assay, morphological observation as well as LDH leakage test were conducted to measure cellular injury. The H2O2-induced cytotoxicity was significantly attenuated in the presence of picroside II (25 microg/ml ) and NGF (2 ng/ml). Cultures with this combined treatment possessed decreased level of ROS while increased cell survival, as compared to that of picroside II or NGF alone-treated cells. Accordingly, it was concluded that their synergistic protective activities against oxidative stress in vitro were demonstrated in various aspects, including reversing morphological changes, enhancing the ability of cell proliferation and ROS scavenging. Such action supports the therapeutic potential of picroside II and NGF in treating nervous disorders based on their synergistic effect.
State Key Laboratory of Bioreactor Engineering & School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China. liujian@ecust.edu.cn
Yan
Y
Cao
Jian-wen
JW
Liu
You-jun
YJ
Yu
Pei-yong
PY
Zheng
Xiao-dong
XD
Zhang
Ting
T
Li
Ming-chuan
MC
Guo
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002934:Cinnamates / Q000494:pharmacology*; D004357:Drug Synergism; D005960:Glucosides / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D057888:Iridoid Glucosides; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D020932:Nerve Growth Factor / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
D002934:Cinnamates; D005960:Glucosides; D057888:Iridoid Glucosides; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; C416837:picroside II; D020932:Nerve Growth Factor; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase