In vitro evaluation of poly(caporlactone) grafted dextran (PGD) nanoparticles with cancer cell.
This study dealt with the preparation and characterization of coumarin-6 loaded poly(caprolactone) grafted dextran (PGD) nanoparticles (NPs) and evaluation of cellular uptake by using human gastric cancer cell line (SNU-638), in vitro. The potential application of these PGD NPs for sustained drug delivery was evaluated by the quantification and localization of the cellular uptake of fluorescent PGD NPs. Coumarin-6 loaded PGD NPs were prepared by a modified oil/water emulsion technique and characterized by various physico-chemical methods such as, laser light scattering for particle size and size distribution, atomic force microscopy (AFM), zeta-potential and spectrofluorometry to identify the release of fluorescent molecules from the NPs. SNU-638 was used to measure the cellular uptake of fluorescent PGD NPs. Confocal laser scanning microscopic images clearly showed the internalization of NPs by the SNU-638 cells. Cell viability was assessed by treating the SNU-638 cells with PGD NPs for 48 h. The results reveal, that these biodegradable polymeric NPs holds promise in biomedical field as a carrier.
Prabu
P
P
Department of Bionanosystem Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Chaudhari
Atul A
AA
Aryal
Santosh
S
Dharmaraj
N
N
Park
S Y
SY
Kim
W D
WD
Kim
H Y
HY
D001672:Biocompatible Materials / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival; D003374:Coumarins / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry*; D016503:Drug Delivery Systems; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000628:therapy; D013844:Thiazoles / Q000737:chemistry
D001672:Biocompatible Materials; D003374:Coumarins; D003911:Dextrans; D005456:Fluorescent Dyes; D011091:Polyesters; D013844:Thiazoles; C517282:coumarin 6; C016240:polycaprolactone