Electrophysiological recordings from the neuromuscular junction of C. elegans.
Electrophysiology provides a quantifiable measure of synaptic activity useful in the functional analysis of synaptic proteins. Recent advances in the application of this technique to C. elegans provides a means of coupling genetics to electrophysiological analysis, providing new insights into the molecular mechanisms regulating neurotransmission. Here we describe a dissection technique that exposes the neuromuscular junctions of C. elegans for electrophysiological analysis. This technique can be adapted to record from virtually any excitable cell in the worm.
Richmond
Janet E
JE
Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA. jer@uic.edu
D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D004594:Electrophysiology / Q000379:methods*; D009469:Neuromuscular Junction / Q000502:physiology*