Evaluation of the ultrasensitive human immunodeficiency virus type 1 (HIV-1) p24 antigen assay performed on dried blood spots for diagnosis of HIV-1 infection in infants.
The diagnostic accuracy of the modified p24 antigen assay performed on pediatric dried blood spots was evaluated. Samples analyzed within 6 weeks of collection yielded no false-positive results (specificity, 100%) and few false-negative results (sensitivity, 96.5% to 98.3%). Laboratory services with limited resources should assess this option for routine infant diagnosis.
Wits Pediatric HIV Clinics, Wits Health Consortium, University of the Witwatersrand, Johannesburg, South Africa.
Janet C
JC
Patton
Ashraf H
AH
Coovadia
Tammy M
TM
Meyers
Gayle G
GG
Sherman
D001769:Blood / Q000821:virology*; D016655:HIV Core Protein p24 / Q000097:blood*; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007223:Infant; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods
D016655:HIV Core Protein p24; C514799:p24 protein, Human Immunodeficiency Virus Type 1