Cohnella laeviribosi sp. nov., isolated from a volcanic pond.
A novel thermophilic and endospore-forming Gram-positive bacterium capable of assimilating and isomerizing l-ribose was isolated from a volcanic area in Likupang, Indonesia. The isolate, RI-39(T), was able to grow at high temperatures (37-60 degrees C); optimum growth was observed at pH 6.5 and 45 degrees C. The strain contained MK-7 (87 %) as the main respiratory quinone and had a DNA G+C content of 51 mol%. The major cellular fatty acids of the isolate were iso-C(16 : 0) and anteiso-C(15 : 0) and the predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysyl-phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate represents an evolutionary lineage that is distinct from those of other Cohnella species. Based on morphological, physiological and chemotaxonomic characteristics and 16S rRNA gene sequence comparisons, it is proposed that strain RI-39(T) represents a novel species, Cohnella laeviribosi sp. nov. The type strain is RI-39(T) (=KCTC 3987(T) =KCCM 10653P(T) =CCUG 52217(T)).
Department of Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea.
Eun-Ah
EA
Cho
Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yusong-gu, Daejeon 305-806, Republic of Korea.
Jung-Sook
JS
Lee
Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yusong-gu, Daejeon 305-806, Republic of Korea.
Keun Chul
KC
Lee
Systems Microbiology Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yusong-gu, Daejeon 305-806, Republic of Korea.|GenoFocus Inc., Yusong, Daejeon 305-811, Republic of Korea.
Heung-Chae
HC
Jung
Systems Microbiology Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yusong-gu, Daejeon 305-806, Republic of Korea.|GenoFocus Inc., Yusong, Daejeon 305-811, Republic of Korea.
Jae-Gu
JG
Pan
Department of Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea.
Yu-Ryang
YR
Pyun
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D016936:Gram-Positive Endospore-Forming Bacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007214:Indonesia; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012266:Ribose / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012266:Ribose