A novel mimovirus vaccine containing survivin epitope with adjuvant IL-15 induces long-lasting cellular immunity and high antitumor efficiency.
Numerous evidences have indicated that CD8+ cytotoxic T lymphocytes (CTLs) played a significant role in protecting host against tumors, thus CTLs have been involved as potentially relevant candidate targets of cancer immunotherapy. Moreover it is crucial to fully elucidate antigen-specific CTL response, especially high-avidity and long-lasting CTL response in vivo. In the present study, we reported a novel tumor vaccine (mimovirus), a viron-size particulate which consisted of a cell-penetrating peptide of Tat(49-57), a CTL epitope peptide survivin(85-93) and a plasmid encoding murine interleukin-15 (IL-15). We demonstrated that this tumor vaccine could effectively mediate the expression of the gene and presentation of the tumor antigen derived peptide ex vivo. Furthermore, the tumor vaccine supported a robust memory CTL-mediated long-term immunity in vivo, which could effectively protect BALB/c mice against fatal CT26 tumor challenge and improve their survival. These findings suggest that the tumor vaccine may provide an alternative therapeutic strategy for cancer patients.
Institute of Immunology, Third Military Medical University, Ave. Gaotanyan 30th, District Shapingba, Chongqing 400038, People's Republic of China.
Zhao
Z
Yang
Li
L
Wang
Huiming
H
Wang
Xiaoyun
X
Shang
Wei
W
Niu
Jintao
J
Li
Yuzhang
Y
Wu
D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D000939:Epitopes; D005260:Female; D015696:Gene Products, tat / Q000276:immunology*; D007111:Immunity, Cellular; D051034:Inhibitor of Apoptosis Proteins; D019409:Interleukin-15 / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008869:Microtubule-Associated Proteins / Q000276:immunology*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000276:immunology*; D009368:Neoplasm Transplantation; D010446:Peptide Fragments / Q000276:immunology; D010957:Plasmids / Q000276:immunology; D000077022:Survivin; D022223:Vaccines, Subunit / Q000276:immunology
C107489:BIRC5 protein, human; D019496:Cancer Vaccines; D000939:Epitopes; D015696:Gene Products, tat; D051034:Inhibitor of Apoptosis Proteins; D019409:Interleukin-15; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D000077022:Survivin; D022223:Vaccines, Subunit