Follicular shuttling of marginal zone B cells facilitates antigen transport.
The splenic marginal zone is a site of blood flow, and the specialized B cell population that inhabits this compartment has been linked to the capture and follicular delivery of blood-borne antigens. However, the mechanism of this antigen transport has remained unknown. Here we show that marginal zone B cells were not confined to the marginal zone but continuously shuttled between the marginal zone and follicular areas, such that many of the cells visited a follicle every few hours. Migration to the follicle required the chemokine receptor CXCR5, whereas return to the marginal zone was promoted by the sphingosine 1-phosphate receptors S1P1 and S1P3. Treatment with an S1P1 antagonist caused displacement of marginal zone B cells from the marginal zone. Marginal zone-follicle shuttling of marginal zone B cells provides an efficient mechanism for systemic antigen capture and delivery to follicular dendritic cells.
Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California, San Francisco, California 94143, USA.
Guy
G
Cinamon
Marcus A
MA
Zachariah
Olivia M
OM
Lam
Frank W
FW
Foss
Jason G
JG
Cyster
D000818:Animals; D000941:Antigens / Q000097:blood / Q000378:metabolism*; D001402:B-Lymphocytes / Q000502:physiology*; D001692:Biological Transport; D020566:Dendritic Cells, Follicular / Q000276:immunology; D000068876:Fingolimod Hydrochloride; D051379:Mice; D018345:Mice, Knockout; D011409:Propylene Glycols / Q000494:pharmacology; D054380:Receptors, CXCR5 / Q000235:genetics / Q000502:physiology; D049349:Receptors, Lysosphingolipid / Q000037:antagonists & inhibitors / Q000502:physiology; D013110:Sphingosine / Q000031:analogs & derivatives / Q000494:pharmacology; D000081025:Sphingosine-1-Phosphate Receptors; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000941:Antigens; D011409:Propylene Glycols; D054380:Receptors, CXCR5; D049349:Receptors, Lysosphingolipid; C501285:S1pr3 protein, mouse; D000081025:Sphingosine-1-Phosphate Receptors; D000068876:Fingolimod Hydrochloride; D013110:Sphingosine