Preferential organ distribution of methylselenol source Se-methylselenocysteine relative to methylseleninic acid.
It has been proposed that Se-methylselenocysteine (MeSeCys) and methylseleninic acid (MSA(IV)) are efficiently transformed through the beta-lyase and reduction reactions, respectively, into methylselenol, the assumed biologically active selenometabolite responsive for the anti-carcinogenicity and anti-oxidant actions of selenium. The bioavailability and distribution of the two selenium sources in major organs/tissues were compared under exactly identical conditions. Namely, labeled selenium sources (76)Se-MeSeCys and (77)Se-MSA(IV), at a single oral dose of 10 microg Se/kg body weight each, were administered simultaneously to rats that had been depleted of natural abundance selenium with a single isotope (78)Se. The same dose of (82)Se-selenite was also administered as a reference selenium source. The distributions of the three labeled selenium isotopes were determined 3 h after the administration in 13 organs/tissues/blood. MeSeCys was taken up more efficiently by most organs, especially the pancreas and duodenum, than MSA(IV) and selenite, the latter two sources being taken up similarly to each other except for in the kidney, liver, and spleen, where the three labeled isotopes were detected at comparable concentrations. The labeled selenium in the liver supernatant was speciated by HPLC inductively coupled argon plasma-mass spectrometry (ICP-MS), and it was suggested that MeSeCys was delivered in its intact form to organs, and then transformed into methylselenol. In addition to the known properties of that MeSeCys is chemically more stable than MSA(IV) and is a naturally occurring edible product, and that MeSeCys produces methylselenol much more efficiently than a homologous selenoamino acid selenomethionine, the present study revealed that MeSeCys is more efficiently distributed than MSA(IV) in its intact form, and then produces methylselenol, suggesting that MeSeCys is the best methylselenol source in most organs/tissues.
Graduate School of Pharmaceutical Sciences, Chiba University, Chuo, Chiba 260-8675, Japan. ktsuzuki@p.chiba-u.ac.jp
Kazuo T
KT
Suzuki
Yoshiro
Y
Tsuji
Yuki
Y
Ohta
Noriyuki
N
Suzuki
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D008297:Male; D013058:Mass Spectrometry; D016566:Organoselenium Compounds / Q000493:pharmacokinetics*; D051381:Rats; D012015:Reference Standards; D017279:Selenocysteine / Q000031:analogs & derivatives; D014018:Tissue Distribution
D016566:Organoselenium Compounds; D017279:Selenocysteine; C008493:methylselenic acid; D003545:Cysteine; C002979:selenomethylselenocysteine