Identification and differentiation of magnetically labeled mesenchymal stem cells in vivo in swines with myocardial infarction.
We aim to track mesenchymal stem cells (MSCs) after magnetically labeling and test the ability of these cells differentiate into cardiomyocytes in vivo. Therefore, 20 swines were divided into four groups, sham-operated group (n=3); acute myocardial infarction (AMI) transplanted with PBS (n=3); labeled MSCs (n=7) and unlabeled MSCs (n=7) group. 10(7) labeled or unlabeled cells were intracoronary delivered after MI (4.8+/-1.3 days), and serial cardiac MR (3.0T) imaging studies were performed at 0, 4 and 8 weeks after transplantation, then the results were confirmed by histological and western blot analysis. We demonstrated that labeled MSCs can be reliably detected and tracked in vivo using MR imaging. In particular, we provided the evidence of regeneration of labeled MSCs in vivo by histological examination and western blot analysis.
Chun-mei
CM
Qi
Gen-shan
GS
Ma
Nai-feng
NF
Liu
Cheng-xing
CX
Shen
Zhong
Z
Chen
Xiao-jun
XJ
Liu
Yao-peng
YP
Hu
Xiao-li
XL
Zhang
Ji-yuan
JY
Chen
Sheng-hong
SH
Ju
Yao-liang
YL
Tang
D000818:Animals; D031057:Arisaema; D002454:Cell Differentiation; D052203:Ferrosoferric Oxide; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008279:Magnetic Resonance Imaging; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D009203:Myocardial Infarction / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000473:pathology*; D013552:Swine
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D052203:Ferrosoferric Oxide