Follicular shuttling of marginal zone B cells facilitates antigen transport.
The splenic marginal zone is a site of blood flow, and the specialized B cell population that inhabits this compartment has been linked to the capture and follicular delivery of blood-borne antigens. However, the mechanism of this antigen transport has remained unknown. Here we show that marginal zone B cells were not confined to the marginal zone but continuously shuttled between the marginal zone and follicular areas, such that many of the cells visited a follicle every few hours. Migration to the follicle required the chemokine receptor CXCR5, whereas return to the marginal zone was promoted by the sphingosine 1-phosphate receptors S1P1 and S1P3. Treatment with an S1P1 antagonist caused displacement of marginal zone B cells from the marginal zone. Marginal zone-follicle shuttling of marginal zone B cells provides an efficient mechanism for systemic antigen capture and delivery to follicular dendritic cells.
Cinamon
Guy
G
Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California, San Francisco, California 94143, USA.
Zachariah
Marcus A
MA
Lam
Olivia M
OM
Foss
Frank W
FW
Cyster
Jason G
JG
D000818:Animals; D000941:Antigens / Q000097:blood / Q000378:metabolism*; D001402:B-Lymphocytes / Q000502:physiology*; D001692:Biological Transport; D020566:Dendritic Cells, Follicular / Q000276:immunology; D000068876:Fingolimod Hydrochloride; D051379:Mice; D018345:Mice, Knockout; D011409:Propylene Glycols / Q000494:pharmacology; D054380:Receptors, CXCR5 / Q000235:genetics / Q000502:physiology; D049349:Receptors, Lysosphingolipid / Q000037:antagonists & inhibitors / Q000502:physiology; D013110:Sphingosine / Q000031:analogs & derivatives / Q000494:pharmacology; D000081025:Sphingosine-1-Phosphate Receptors; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000941:Antigens; D011409:Propylene Glycols; D054380:Receptors, CXCR5; D049349:Receptors, Lysosphingolipid; C501285:S1pr3 protein, mouse; D000081025:Sphingosine-1-Phosphate Receptors; D000068876:Fingolimod Hydrochloride; D013110:Sphingosine