[Dot enzyme-linked immunosorbent assay for detection of serum antibody to Blastocystis hominis in humans].
Serum and stool samples were collected from 322 undergraduate students in medical school. Using stool in vitro cultivation as golden standard, 178 cases were found Blastocystis hominis positive and 144 were negative. Dot-ELISA was used to examine the serum samples with a sensitivity of 92.1% (164/178) and specificity of 97.1% (141/144). This revealed that dot-ELISA can be used for antibody detection against Blastocystis hominis.
Su
Shui-lian
SL
Gannan Medical College, Ganzhou 341000, China.
Yan
Yi-ming
YM
Liao
Hua
H
Chen
Gui-feng
GF
Zhang
Rui-qi
RQ
Xie
Qiong-jun
QJ
Le
Xiao
X
Hu
Ya-qiong
YQ
Zeng
Xue-ying
XY
Lan
Hai-ying
HY
Xie
Rui-lian
RL
Huang
Zhen
Z
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D016776:Blastocystis Infections / Q000097:blood / Q000175:diagnosis / Q000469:parasitology*; D016845:Blastocystis hominis / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000469:parasitology; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000913:Antibodies, Protozoan