Exopolysaccharide production and mycelial growth in an air-lift bioreactor using Fomitopsis pinicola.
For effective exopolysaccharide production and mycelial growth by a liquid culture of Fomitopsis pinicola in an air-lift bioreactor, the culture temperature, pH, carbon source, nitrogen source, and mineral source were initially investigated in a flask. The optimal temperature and pH for mycelial growth and exopolysaccharide production were 25degrees C and 6.0, respectively. Among the various carbon sources tested, glucose was found to be the most suitable carbon source. In particular, the maximum mycelial growth and exopolysaccharide production were achieved in 4% glucose. The best nitrogen sources were yeast extract and malt extract. The optimal concentrations of yeast extract and malt extract were 0.5 and 0.1%, respectively. K2HPO4 and MgSO4 x 7H2O were found to be the best mineral sources for mycelial growth and exopolysaccharide production. In order to investigate the effect of aeration on mycelial growth and exopolysaccharide production in an air-lift bioreactor, various aerations were tested for 8 days. The maximum mycelial growth and exopolysaccharide production were 7.9 g/l and 2.6 g/l, respectively, at 1.5 vvm of aeration. In addition, a batch culture in an air-lift bioreactor was carried out for 11 days under the optimal conditions. The maximum mycelial growth was 10.4 g/l, which was approximately 1.7-fold higher than that of basal medium. The exopolysaccharide production was increased with increased culture time. The maximum concentration of exopolysaccharide was 4.4 g/l, which was about 3.3-fold higher than that of basal medium. These results indicate that exopolysaccharide production increased in parallel with the growth of mycelium, and also show that product formation is associated with mycelial growth. The developed model in an air-lift bioreactor showed good agreement with experimental data and simulated results on mycelial growth and exopolysaccharide production in the culture of F pinicola.
Biotechnology Laboratory, B-K Company Ltd., Gunsan 573-879, Korea.
Dubok
D
Choi
Jeung-Moo
JM
Maeng
Ji-Lu
JL
Ding
Wol-Suk
WS
Cha
D000332:Aerobiosis; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008278:Magnesium Sulfate / Q000378:metabolism; D025282:Mycelium / Q000254:growth & development*; D009584:Nitrogen / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D020072:Polyporales / Q000254:growth & development / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis*; D017680:Potassium Compounds / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D010710:Phosphates; D011134:Polysaccharides; D017680:Potassium Compounds; D002244:Carbon; D008278:Magnesium Sulfate; C013216:potassium phosphate; D009584:Nitrogen