Competitive and product inhibition-based alpha-amylase activity analysis method.
OBJECTIVE
To propose a methodology for analyzing salivary alpha-amylase activity (sAMY) for a hand-held device that can be used easily and quickly for evaluating human psychological effects.


METHODS
An improved method for the analysis of sAMY is proposed using competitive and product inhibition in a dry-chemistry system with a reagent paper containing 2-chloro-4-nitrophenyl-4-O-beta-D-galactopyranosylmaltoside.


RESULTS
Not only the competitive (maltopentaose) inhibitor, but also the product (maltotriose) inhibitor significantly reduce the reaction speed of sAMY. There is an independent effect between the two inhibitors. The dynamic linear range of the analysis can be enhanced by 2.5 times larger by adding the competitive and product inhibitors simultaneously and by preparing their concentrations appropriately.


CONCLUSIONS
It is considered that the application of competitive and product inhibition can be effective from the viewpoint of enhancing analysis range and effectively reducing costs.
Graduate School of Science and Engineering for Research, University of Toyama, Toyama, Japan. yamag@eng.u-toyama.ac.jp
Masaki
M
Yamaguchi
Junichi
J
Wakasugi
Josaku
J
Sakakima
D002624:Chemistry, Clinical / Q000295:instrumentation / Q000379:methods*; D004789:Enzyme Activation; D008954:Models, Biological; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D014312:Trisaccharides / Q000378:metabolism; D000516:alpha-Amylases / Q000737:chemistry / Q000378:metabolism*
C411063:2-chloro-4-nitrophenyl-4-O-galactopyranosylmaltoside; D009844:Oligosaccharides; D014312:Trisaccharides; C019193:maltopentaose; C008317:maltotriose; D000516:alpha-Amylases