Isolation and structure determination of streptochlorin, an antiproliferative agent from a marine-derived Streptomyces sp. 04DH110.
An antiproliferative agent, streptochlorin, was isolated from the fermentation broth of a marine actinomycete isolated from marine sediment. Phylogenetic analysis of the 16S rRNA gene sequence indicated that the strain belongs to the genus Streptomyces. Bioactivity guided fractionation of the culture extract by solvent partitioning, ODS open flash chromatography, and reversed-phase HPLC gave a pure compound, streptochlorin. Its structure was elucidated by extensive 2D NMR and mass spectral analyses. Streptochlorin exhibited significant antiproliferative activity against human cultured cell lines.
Shin
Hee Jae
HJ
Marine Natural Products Laboratory, Korea Ocean Research & Development Institute, Ansan, P O. Box 29, Seoul 425-600, Korea. shinhj@kordi.re.kr
Jeong
Hyun Sun
HS
Lee
Hyi-Seung
HS
Park
Song-Kyu
SK
Kim
Hwan Mook
HM
Kwon
Ho Jeong
HJ
D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D002478:Cells, Cultured; D005591:Chemical Fractionation; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D006801:Humans; D007211:Indoles / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010080:Oxazoles / Q000302:isolation & purification / Q000494:pharmacology*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013302:Streptomyces / Q000737:chemistry* / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D000970:Antineoplastic Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007211:Indoles; D010080:Oxazoles; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C526070:streptochlorin