[A new chemical component of Pueraria lobata (Willd.) Ohwi].
Pueraria lobata (Willd.) Ohwi. was extracted for two times with 70% ethanol and the 70% ethanol-extracts was condensed. Various column chromatography with AB-8 macroreticular resin, Toyopearl HW-40, pharmadex LH-20, and silica gel were employed for the isolation and purification of the 70% ethanol-extracts from Pueraria lobata (Willd.) Ohwi. Five compounds were isolated and their structures were identified by physiochemical properties and spectral analysis (UV, IR, MS, 1H NMR, 13C NMR, HMQC, HMBC, etc.): (4R)-3-[ 2-hydroxy-4-methoxyphenyl]-4-(4-beta-D-glucopyranosyloxybenzyl) but-2-en-4-olide (1), 4', 8-dimethoxyl-7-O-beta-D-glucopyranosyl isoflavone (2), eicosanoic acid (3), hexadecanoic acid (4), tetracosanoid acid-2,3-dihydroxypropyl ester (5). Compound 1 was a new compound, and compounds 2, 3, 4 were isolated from this plant for the first time.
School of Pharmacy, Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China. wangyzlb@126.com
Yan-Zhi
YZ
Wang
Wei-Sheng
WS
Feng
Ren-Bing
RB
Shi
Bin
B
Liu
D004537:Eicosanoic Acids / Q000737:chemistry / Q000302:isolation & purification; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D019308:Palmitic Acid / Q000737:chemistry / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D029908:Pueraria / Q000737:chemistry*
D004537:Eicosanoic Acids; D005960:Glucosides; C542305:puerarinoid D; D019308:Palmitic Acid