Neurophysiological methods in C. elegans: an introduction.
The simple and well-defined structure of the C. elegans nervous system has made it an attractive model for studying the neural and genetic basis of behavior. However, the wider use physiological methods for monitoring neural activity in vivo or determining the effects of specific ion channels on neuronal function has been a relatively recent development. This chapter presents a compendium of protocols and technical reports on the current state of the art in C. elegans electrophysiology and neuroimaging. These include methods for calcium imaging in intact animals, in situ electrical recording from neurons and muscle cells, and in vitro recording from cultured neurons and oocytes.
Schafer
William R
WR
Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093-0634, USA.
D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D005456:Fluorescent Dyes; D009482:Neurophysiology / Q000379:methods*
D005456:Fluorescent Dyes