Analysis of fibroblast growth factor influence on growth and developmental potential of rat foetuses in the in vitro culture model.
The fibroblast growth factor's (FGF) influence on the growth and differentiation of 8- and 9- day-old rat foetus has been studied, whereas foetuses were grown in an in vitro culture model. Proliferation was analysed by the expression of proliferating cell nuclear antigen (PCNA). It was established that the usage of FGF in the first period of the culture lowers the growth no matter the foetus age at the moment of culturing and no matter whether it is a medium with or without a serum. If FGF is applied in the second culture period, it also lowers the growth, however younger foetuses in the in vitro culture model are more sensible to FGF negative influence. When FGF was applied in a lower concentration the growth of whole foetuses was improved in the in vitro culture model, which shows that the FGF influence on growth depends on the concentration. Stereological analyses have been done and showed that, in the in vitro culture model, FGF has no influence on proliferating cartilage tissue, but it stimulates the survival of nervel tissue cells. It has been shown that the quantitative research of growth processes in cultivated foetuses can precisely be done by combining classic methods of measuring whole foetus diameters and analysing the expression of proliferating antigen.
Marić
Svjetlana
S
School o Medicine, University of Osijek, Osijek, Croatia. svjetlanamaric@yahoo.com
Bulić-Jakus
Floriana
F
Belovari
Tatjana
T
Krcmar
Stjepan
S
D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation / Q000502:physiology*; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D005346:Fibroblast Growth Factors / Q000502:physiology*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344
D015415:Biomarkers; D018809:Proliferating Cell Nuclear Antigen; D005346:Fibroblast Growth Factors