[Effects of interleukin-1beta on nitric oxide production and ATP synthesis in rat surplus hepatocytes after partial hepatectomy].
OBJECTIVE
To detect the nitric oxide (NO) production and energy metabolism of the interleukin (IL)-1beta-treated residual hepatocytes from rats after partial hepatectomy.


METHODS
Forty rats were equally divided into partial hepatectomies (PH) group and control group. In the control group the rats were otherwise matched and underwent sham surgeries. The residual hepatocytes were separated by the collagenase perfusion method. The hepatocytes were cultured with cytokines such as IL-1beta. The production of NO in the two groups were measured with Griess reagent method, the production of inducible nitric oxide synthase (iNOS) protein detected with Western blot, the content of the nucleotide in the hepatocytes detected with high-performance liquid chromatography, and the content of the ketone body in the hepatocytes of the two groups determined with the enzymatic method. Afterwards the ketone body ratio (acetoacetate/beta-hydroxy butyrate, KBR) was calculated.


RESULTS
The production of NO in the PH group was twice as much as that in the Sham group. IL-1beta decreased the content of ATP and the KBR in the hepatocytes of both groups, and the decrease magni tude in the PH group was significantly larger than that in the Sham group. After the injection of L-arginine, the production of NO in the hepatocytes in the PH group increased, and the level of ATP and KBR decreased. N(G)-methyl-L-arginine (L-NMMA), the inhibitor of NO synthase, inhibited the production of NO and reversed the decrease of ATP and KBR.


CONCLUSIONS
After partial hepatectomy, increased NO production in the hepatocytes after the treatment of interleukin-1beta may disturb the function of mitochondria by inhibiting the synthesis of ATP.
Department of General Surgery, Fourth Affiliated Hospital of China Medical University, Shenyang 110032, China. tuwei77@hotmail.com
Wei
W
Tu
Song
S
Hu
Man
M
Zhao
Wen-zhi
WZ
Qu
Zuo-fu
ZF
Yu
Wen-yu
WY
Sun
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002478:Cells, Cultured; D006498:Hepatectomy; D022781:Hepatocytes / Q000378:metabolism*; D053583:Interleukin-1beta / Q000494:pharmacology; D007657:Ketone Bodies / Q000096:biosynthesis; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D051381:Rats; D019323:omega-N-Methylarginine / Q000494:pharmacology
D053583:Interleukin-1beta; D007657:Ketone Bodies; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D000255:Adenosine Triphosphate; D001120:Arginine; D019001:Nitric Oxide Synthase