[Development of a rapid method for the detection of prostate-specific antigen by immunochromatography].
A single-step qualitative rapid test for the determination of prostate-specific antigen (PSA) in samples of human blood serum by immunochromatography using a complex of colloidal gold with monoclonal antibodies to PSA as the detection agent was developed. The determination limit for PSA in serum blood samples is 10 ng/ml; the analysis time, 15-25 min; the sensitivity of the method, 100%; and its specificity, 92.5%.
I A
IA
Liubavina
A A
AA
Zinchenko
Iu S
IuS
Lebedin
S V
SV
Chukanov
D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay; D008297:Male; D017430:Prostate-Specific Antigen / Q000097:blood*; D011471:Prostatic Neoplasms / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D006046:Gold; D017430:Prostate-Specific Antigen