Differential expression of papillomavirus L1 proteins encoded by authentic and codon modified L1 genes in methylcellulose-treated mouse keratinocytes.
Papillomaviruses (PVs) are double-stranded DNA viruses that infect keratinocytes in differentiating epithelia and induce hyperproliferative lesions. Here, we used methylcellulose to induce cell differentiation of primary mouse keratinocytes (KCs) in in vitro culture and assessed the expression of authentic and codon-modified version of L1 capsid genes from two PV types (HPV6b and BPV1). Based on the quantitative RT-PCR analysis, methylcellulose treatment did not influence the transcriptional expression of both authentic and codon-modified L1 genes in KCs. Western blot showed that methylcellulose significantly increased the levels of the L1 proteins expressed from two authentic L1 genes. Conversely, methylcellulose dramatically decreased L1 protein expression in KCs transfected with two codon-modified L1 expression constructs. These data suggest that L1 protein expression is associated with KC differentiation induced by methylcellulose treatment and regulated at the post-transcriptional level.
Wang
Xiao
X
Diamantina Institute for Cancer, Immunology & Metabolic Medicine, University of Queensland, Research Extension, Building 1, Princess Alexandra Hospital, Woolloongabba, Queensland 4102, Australia. xxxwang1@uq.edu.au
Li
Bo
B
Zhao
Kong-Nan
KN
D000818:Animals; D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D003062:Codon; D015967:Gene Expression Regulation, Viral; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology / Q000821:virology; D008747:Methylcellulose / Q000494:pharmacology*; D051379:Mice; D008856:Microscopy, Fluorescence; D027383:Papillomaviridae / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D036022:Capsid Proteins; D003062:Codon; C085217:L1 protein, Bovine papillomavirus; D008747:Methylcellulose