The ensemble of hetero-proteins in inorganic nanochannels.
The assembly and proper alignment of two heterofluorescent proteins (sGFP and DsRed) in the mesoporous channels of ethanol-treated FSM6.2 (a folded-sheet mesoporous material with a pore diameter of 6.2 nm) was confirmed using a fluorescence resonance energy transfer (FRET) technique. The sGFP-DsRed-FSM6.2 conjugate showed a large decrease in the emission of donor (sGFP) fluorescence, indicating that the conjugate functions as an energy transfer system through the combination of the two heteroproteins, due to the successful encapsulation of the sGFP-DsRed pairs in the mesopores. Fluorescence spectral analysis demonstrated that the proteins were highly dispersed and homogeneously encapsulated in the mesopores of FSM6.2, even at high concentration, although they spontaneously aggregated and showed a red shift in solution at the concentration corresponding to that in the conjugate. Furthermore, an increase in the amount of sGFP and DsRed adsorbed to the pores of FSM6.2 led to a decrease in the distance between these proteins, resulting in enhancement of FRET efficiency.
Research Center for Compact Chemical Process, National Institute of Advanced Industrial Science and Technology, Nigatake 4-2-1, Miyagino-ku, Sendai 983-8551, Japan. matsuura-shunichi@aist.go.jp
Shun-ichi
S
Matsuura
Tetsuji
T
Itoh
Ryo
R
Ishii
Kengo
K
Sakaguchi
Tatsuo
T
Tsunoda
Takaaki
T
Hanaoka
Fujio
F
Mizukami
D031541:Fluorescence Resonance Energy Transfer; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D007287:Inorganic Chemicals / Q000737:chemistry*; D008164:Luminescent Proteins / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D016062:Porosity; D017640:Silicates / Q000737:chemistry; D000097573:Red Fluorescent Protein
D049452:Green Fluorescent Proteins; D007287:Inorganic Chemicals; D008164:Luminescent Proteins; D017640:Silicates; D000097573:Red Fluorescent Protein