In vitro cytotoxicity of single-walled carbon nanotube/biodegradable polymer nanocomposites.
Injectable nanocomposites made of biodegradable poly(propylene fumarate) and the crosslinking agent propylene fumarate-diacrylate as well as each of three forms of single-walled carbon nanotubes (SWNTs) were evaluated for their in vitro cytotoxicity. Unreacted components, crosslinked networks, and degradation products of the nanocomposites were investigated for their effects on cell viability using a fibroblast cell line in vitro. The results did not reveal any in vitro cytotoxicity for purified SWNTs, SWNTs functionalized with 4-tert-butylphenylene, and ultra-short SWNTs at 1- 100 microg/mL concentrations. Moreover, nearly 100% cell viability was observed on all crosslinked nanocomposites and cell attachment on their surfaces was comparable with that on tissue culture polystyrene. The degradation products of the nanocomposites displayed a dose-dependent adverse effect on cells, which was partially due to increased osmolarity by the conditions of accelerated degradation and could be overcome at diluted concentrations. These results demonstrate that all three tested nanocomposites have favorable cytocompatibility for potential use as scaffolds for bone tissue engineering applications.
Department of Bioengineering, Rice University, MS-142, Houston, Texas 77251-1892, USA.
Xinfeng
X
Shi
Balaji
B
Sitharaman
Quynh P
QP
Pham
Patrick P
PP
Spicer
Jared L
JL
Hudson
Lon J
LJ
Wilson
James M
JM
Tour
Robert M
RM
Raphael
Antonios G
AG
Mikos
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects*; D005453:Fluorescence; D018613:Microscopy, Confocal; D053761:Nanocomposites; D037742:Nanotubes, Carbon; D011108:Polymers / Q000494:pharmacology*; D051381:Rats
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D037742:Nanotubes, Carbon; D011108:Polymers