Enhancing polychlorinated biphenyl dechlorination in fresh water sediment with biostimulation and bioaugmentation.
Polychlorinated biphenyls (PCBs) are toxic compounds ubiquitously distributed in ecosystems. Microbial attenuation of these contaminants is a potential means of remediation. Two promising microbial PCB remediation technologies, biostimulation and bioaugmentation, were investigated in different sediments. Biostimulation experiments in which electron donor was supplied (H2 via elemental iron, Fe(0)) resulted in only a marginal improvement in the dechlorination of amended 2,3,4,5-tetrachlorobiphenyl (2,3,4,5-CB), likely because of an inadequate population of indigenous H2-utilizing dechlorinators. Extensive dechlorination was observed, however, after bioaugmenting microcosms with a PCB-dechlorinating enrichment culture. Dechlorination of 2,3,4,5-CB began prior to the 20th day of incubation and proceeded to 2-chlorobiphenyl. This extensive dechlorination activity was maintained in both sediments over 70d at 10 and 25 degrees C. This research demonstrates that although past studies of biostimulation were promising, a great deal must be known about the PCB-dechlorinating organisms present before successful biostimulation is expected. Bioaugmentation, however, appears to be a promising PCB remediation strategy and should be further pursued.
Winchell
Lloyd J
LJ
The Department of Civil Engineering, University of Minnesota, 122 Civil Engineering Building, 500 Pillsbury Drive S.E., Minneapolis, MN 55455-0220, United States.
Novak
Paige J
PJ
D002713:Chlorine / Q000378:metabolism*; D005618:Fresh Water; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D014874:Water Pollutants, Chemical; D002713:Chlorine; D011078:Polychlorinated Biphenyls