[Expression profile analysis of host HeLa cells invasived by Shigella flexneri 2a].
The changes of genes expression in HeLa cell during the invasion with Shigella species for 1h and 3h were analyzed by cDNA microarrays. The data showed that the expression levels of 752 genes were altered twice or greater as compared with the control 509 of them were up-regulated, and 306 were down-regulated. It was supposed that some signal pathways in HeLa cell were activated, then many genes were induced, and at last comprehensive cell responses were produced, so that HeLa cell could prevent against Shigella species infection. Two striking difference cDNA fragments TNFR 1B and ERBB2, which were up-regulated in the host epithelial cell during Shigella infection, analyzed expression by real time RT-PCR as determined by cDNA arrays. We suggested they play important roles in response to the invasive S. flexneri 2457T. Based on the results of gene expression alterations, the molecular pathogenic mechanism of Shigella species could be greatly and deeply understood, and the strategy for prevention against and treatment for shigellosis would be formed.
Huang
Liu-Yu
LY
Department of Microbiology, Third Military Medical University, Chongqing 400038, China. huangliuyuly@163.com
Shi
Zhao-Xing
ZX
Yuan
Jing
J
Hu
Fu-Quan
FQ
D000242:Cyclic AMP / Q000502:physiology; D020869:Gene Expression Profiling; D006367:HeLa Cells; D006801:Humans; D020935:MAP Kinase Signaling System; D020411:Oligonucleotide Array Sequence Analysis; D011493:Protein Kinase C / Q000502:physiology; D043562:Receptors, G-Protein-Coupled / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012763:Shigella flexneri / Q000145:classification / Q000472:pathogenicity*
D043562:Receptors, G-Protein-Coupled; D000242:Cyclic AMP; D011493:Protein Kinase C