Synthesis of 5'-amino-5'-deoxyguanosine-5'-N-phosphoramidate and its enzymatic incorporation at the 5'-termini of RNA molecules.
5'-amino-5'-deoxyguanosine-5'-N-phosphoramidate (GNHP) was synthesized in four steps from guanosine and was found to initiate T7 RNAP-promoted transcriptions to afford 5'-H2N-RNA that can be conjugated to activated esters.
Department of Chemistry, University Science Laboratories, South Road, Durham, UK DH1 3LE.
David
D
Williamson
Martin J
MJ
Cann
David R W
DR
Hodgson
D001710:Biotin / Q000737:chemistry; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012972:Sodium Hydroxide / Q000737:chemistry; D012996:Solutions; D014158:Transcription, Genetic / Q000235:genetics
C526213:5'-amino-5'-deoxyguanosine-5'-N-phosphoramidate; D012996:Solutions; D012972:Sodium Hydroxide; D012313:RNA; D001710:Biotin; D003849:Deoxyguanosine