Matrix metalloproteinase-2, -9, and -12 gene polymorphisms in generalized aggressive periodontitis.
BACKGROUND
Matrix metalloproteinases (MMPs) are involved in periodontal tissue remodeling and degradation. Polymorphisms in the promoter region of the MMP-2 and -9 genes and in the coding region of the MMP-12 gene could affect transcription and the function of these enzymes. The aim of the present study was to determine the association between the aforementioned MMP polymorphisms and generalized aggressive periodontitis (GAgP).


METHODS
Genomic DNA was obtained from the peripheral blood of 92 subjects with GAgP and 157 periodontally healthy subjects. MMP-2 -735C/T, MMP-9 -1562C/T, and MMP-12 357Asn/Ser polymorphisms were genotyped by polymerase chain reaction and restriction fragment length polymorphism. Probing depth, clinical attachment loss, supragingival plaque accumulation, and bleeding on probing were recorded. The data were analyzed by chi(2), logistic regression, and Mann-Whitney U tests.


RESULTS
The genotype distributions, allele frequencies, and rare allele carriage of MMP-2 and MMP-12 genes were similar in GAgP and healthy subjects (P >0.05). T allele frequency and T allele carriage of the MMP-9 -1562 C/T polymorphism were significantly lower in the GAgP group than in the healthy group (P <0.05). In addition, logistic regression analysis revealed a protective effect for MMP-9 -1562 T allele carriers (odds ratio = 0.52; P = 0.04).


CONCLUSIONS
MMP-2 -735C/T and MMP-12 357Asn/Ser polymorphisms are not related to GAgP. Conversely, the MMP-9 -1562 gene T allele might be associated with a decreased risk for GAgP in the Turkish population.
Gürkan
Ali
A
Department of Periodontology, School of Dentistry, Ege University, Izmir, Turkey. gurkan@ege.edu.tr
Emingil
Gülnur
G
Saygan
Buket Han
BH
Atilla
Gül
G
Cinarcik
Serhat
S
Köse
Timur
T
Berdeli
Afig
A
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D016015:Logistic Models; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D020125:Mutation, Missense; D010518:Periodontitis / Q000201:enzymology* / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D018709:Statistics, Nonparametric
D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9