Tandem MS can distinguish hyaluronic acid from N-acetylheparosan.
Isobaric oligosaccharides enzymatically prepared from hyaluronic acid (HA) and N-acetylheparosan (NAH), were distinguished using tandem mass spectrometry. The only difference between the two series of oligosaccharides was the linkage pattern (in HA 1-->3 and in NAH 1-->4) between glucuronic acid and N-acetylglucosamine residues. Tandem mass spectrometry afforded spectra in which glycosidic cleavage fragment ions were observed for both HA and NAH oligosaccharides. Cross-ring cleavage ions 0,2An and 0,2An-h (n is even number) were observed only in GlcNAc residues of NAH oligosaccharides. One exception was an 0,2A2 ion fragment observed for the disaccharide from HA. These cross-ring cleavage fragment ions are useful to definitively distinguish HA and NAH oligosaccharides.
Zhang
Zhenqing
Z
Department of Chemistry, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
Xie
Jin
J
Liu
Jian
J
Linhardt
Robert J
RJ
D000942:Antigens, Bacterial / Q000737:chemistry; D000954:Antigens, Surface / Q000737:chemistry; D006025:Glycosaminoglycans / Q000032:analysis*; D006820:Hyaluronic Acid / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D006025:Glycosaminoglycans; C030659:K antigens; C527794:N-acetylheparosan; D006820:Hyaluronic Acid