Human mulipotential mesenchymal/stromal stem cells are derived from a discrete subpopulation of STRO-1bright/CD34 /CD45(-)/glycophorin-A-bone marrow cells.
Magnetic and flow cytometry-based methods were used to characterize clonogenic stromal cells in human bone marrow. STRO-1(bright) stromal cells were found to lack expression of CD34, CD45 and glycophorin-A markers associated with hematopoietic progenitor cells. These studies support the view that these are two distinct stem cell compartments in adult bone marrow.
Zannettino
Andrew C W
AC
Multiple Myeloma Research Laboratory, Division of Haematology, Institute of Medical and Veterinary Science, Hanson Institute and University of Adelaide, Australia.
Paton
Sharon
S
Kortesidis
Angela
A
Khor
Fiona
F
Itescu
Silviu
S
Gronthos
Stan
S
D018952:Antigens, CD34; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D001853:Bone Marrow; D005434:Flow Cytometry; D006021:Glycophorins; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D018189:Immunomagnetic Separation; D017493:Leukocyte Common Antigens; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D039902:Multipotent Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology
D018952:Antigens, CD34; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D006021:Glycophorins; C525591:STRO-1 antigen, human; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human