Effects of verapamil and gadolinium on caffeine-induced contractures and calcium fluxes in frog slow skeletal muscle fibers.
In this work, we tested whether L-type Ca(2+ )channels are involved in the increase of caffeine-evoked tension in frog slow muscle fibers. Simultaneous net Ca(2+) fluxes and changes in muscle tension were measured in the presence of caffeine. Isometric tension was recorded by a mechanoelectrical transducer, and net fluxes of Ca(2+) were measured noninvasively using ion-selective vibrating microelectrodes. We show that the timing of changes in net fluxes and muscle tension coincided, suggesting interdependence of the two processes. The effects of Ca(2+)channel blockers (verapamil and gadolinium) were explored using 6 mM: caffeine; both significantly reduced the action of caffeine on tension and on calcium fluxes. Both caffeine-evoked Ca(2+) leak and muscle tension were reduced by 75% in the presence of 100 microM: GdCl(3), which also caused a 92% inhibition of net Ca(2+) fluxes in the steady-state condition. Application of 10 microM: verapamil to the bath led to 30% and 52% reductions in the Ca(2+)leak caused by the presence of caffeine for the peak and steady-state values of net Ca(2+) fluxes, respectively. Verapamil (10 microM): caused a 30% reduction in the maximum values of caffeine-evoked muscle tension. Gd(3+)was a more potent inhibitor than verapamil. In conclusion, L-type Ca(2+) channels appear to play the initial role of trigger in the rather complex mechanism of slow fiber contraction, the latter process being mediated by both positive Ca(2+)-induced Ca(2+ )release and negative (Ca(2+) removal from cytosol) feedback loops.
Shabala
Lana
L
School of Agricultural Science, University of Tasmania, Hobart, Tasmania 7001, Australia. L.Shabala@utas.edu.au
Sánchez-Pastor
Enrique
E
Trujillo
Xóchitl
X
Shabala
Sergey
S
Muñiz
Jesús
J
Huerta
Miguel
M
D000818:Animals; D001001:Anura; D002110:Caffeine; D002118:Calcium / Q000378:metabolism*; D003286:Contracture / Q000139:chemically induced / Q000503:physiopathology / Q000517:prevention & control*; D005682:Gadolinium / Q000494:pharmacology*; D017136:Ion Transport / Q000187:drug effects; D008954:Models, Biological; D018657:Muscle Fibers, Slow-Twitch / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014700:Verapamil / Q000494:pharmacology*
D002110:Caffeine; D005682:Gadolinium; D014700:Verapamil; D002118:Calcium