Ostreolysin enhances fruiting initiation in the oyster mushroom (Pleurotus ostreatus).
Fruiting initiation in mushrooms can be triggered by a variety of environmental and biochemical stimuli, including substances of natural or synthetic origin. In this work ostreolysin, a cytolytic protein specifically expressed during the formation of primordia and fruit bodies of Pleurotus ostreatus, was applied to nutrient media inoculated with mycelium of P. ostreatus, and its effects on mycelial growth and fructification of the mushroom studied. The addition of ostreolysin slightly inhibited the growth of mycelium, but strongly induced the formation of primordia, which appeared 10 d earlier than in control plates supplemented with bovine serum albumin or with the dissolving buffer alone. Moreover, ostreolysin stimulated the subsequent development of primordia into fruit bodies. However, direct involvement of this protein in the sporulation of the mushroom is unlikely, as it was also detected in large amounts in the non-sporulating strain of P. ostreatus.
Medical centre for molecular biology, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia.
Sabina
S
Berne
Jure
J
Pohleven
Iztok
I
Vidic
Katja
K
Rebolj
Franc
F
Pohleven
Tom
T
Turk
Peter
P
Macek
Anton
A
Sonnenberg
Kristina
K
Sepcić
D000818:Animals; D002417:Cattle; D003470:Culture Media; D004912:Erythrocytes / Q000502:physiology; D048690:Fruiting Bodies, Fungal / Q000187:drug effects* / Q000254:growth & development*; D005656:Fungal Proteins / Q000378:metabolism / Q000494:pharmacology; D006460:Hemolysin Proteins / Q000378:metabolism / Q000494:pharmacology*; D006461:Hemolysis; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development; D020076:Pleurotus / Q000187:drug effects* / Q000254:growth & development*
D003470:Culture Media; D005656:Fungal Proteins; D006460:Hemolysin Proteins; C461280:ostreolysin