Aptamer biosensor for protein detection using gold nanoparticles.
Combining gold nanoparticles (GNPs) as fluorescence quencher and aptamer as probe, we have developed protein biosensors by using DNA-modified GNPs. We examined how the experimental design, such as the type of interaction between DNA strands and GNPs, temperature, and microenvironment of aptamer, influences the recognition ability of the biosensor. Under our experimental conditions, the recognition of protein by the complex of dye-labeled DNA hybridized with aptamer that is immobilized on GNPs (Ap-Im-GNPs) shows the best character in protein detection.
Wang
Wenjuan
W
Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Chen
Chunlai
C
Qian
Minxie
M
Zhao
Xin Sheng
XS
D052157:Aptamers, Nucleotide; D052158:Aptamers, Peptide; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013917:Thrombin / Q000032:analysis
D052157:Aptamers, Nucleotide; D052158:Aptamers, Peptide; D006046:Gold; D004247:DNA; D013917:Thrombin