Cytoplasmic proteome reference map for a glutamic acid-producing Corynebacterium glutamicum ATCC 14067.
We constructed a cytoplasmic proteome reference map for a glutamic acid producing Corynebacterium glutamicum ATCC 14067 by 2-DE and protein identification by MALDI-TOF-MS and PMF using genome database of the type strain ATCC 13032. The map allowed us to identify 166 protein spots representing 139 different proteins. A considerable strain difference was observed in the proteomic images between strains ATCC 14067 and ATCC 13032 grown under the glutamic acid production conditions, suggesting the importance of strain-specific reference map for proteomic analysis.
Laboratory of Microbial Physiology, Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Hokkaido, Japan.
Liyuan
L
Li
Masaru
M
Wada
Atsushi
A
Yokota
D001426:Bacterial Proteins / Q000032:analysis*; D048230:Corynebacterium glutamicum / Q000737:chemistry* / Q000378:metabolism; D003593:Cytoplasm / Q000737:chemistry; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D018698:Glutamic Acid / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D020543:Proteome; D018698:Glutamic Acid