Identification of interleukin-1beta regulated genes in uterine smooth muscle cells.
We analyzed the response of uterine smooth muscle cells to interleukin-1beta (IL-1beta). We first showed that PHM1-31 myometrial cells, our cellular model, are contractile. To determine the molecular mechanisms of uterine smooth muscle cell activation by proinflammatory cytokines, we performed genechip expression array profiling studies of PHM1-31 cells in the absence and the presence of IL-1beta. In total, we identified 198 known genes whose mRNA levels are significantly modulated (> 2.0-fold change) following IL-1beta exposure. We confirmed the expression changes for selected genes by independent mRNA and protein analysis. The group of genes induced by IL-1beta includes transcription factors and inflammatory response genes such as nuclear factor of kappa light polypeptide gene enhancer in B-cells (NFkappaB), pentraxin-related gene (PTX3), and tumor necrosis factor alpha-induced protein 3/A20 (TNFAIP3/A20). We also found up-regulation of chemokines like C-X-C motif ligand 3 (CXCL3) and extracellular matrix remodeling signaling molecules like tenascin C (TNC). Our data suggest that IL-1beta elicits the rapid activation of a cellular network of genes particularly implicated in inflammatory response that may create a cellular environment favorable for myometrial cell contraction. Our results provide novel insights into the mechanisms of uterine smooth muscle cell regulation and possibly infection-induced preterm labor.
Chevillard
Grégory
G
Lady Davis Institute for Medical Research, McGill University, 3755 Cote Sainte-Catherine Road, Montreal, Quebec, H3T 1E2, Canada.
Derjuga
Anna
A
Devost
Dominic
D
Zingg
Hans H
HH
Blank
Volker
V
D015152:Blotting, Northern / Q000379:methods; D002460:Cell Line; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D053583:Interleukin-1beta / Q000494:pharmacology*; D009200:Myocardial Contraction; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000276:immunology*; D009215:Myometrium / Q000187:drug effects / Q000276:immunology*; D007752:Obstetric Labor, Premature / Q000276:immunology*; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D013268:Stimulation, Chemical
D053583:Interleukin-1beta