Proteome analysis of Persian sturgeon (Acipenser persicus) ova.
The Persian sturgeon ova are a key material both for inevitable artificial propagation and for caviar production. In this study, the proteome profile of Persian sturgeon ova was analyzed using 2-DE and MALDI-TOF/TOF in order to determine its protein composition. Out of 192 spots analyzed with MALDI-TOF/TOF, 107 spots corresponding to 73 different proteins were identified. The identified proteins were classified into 11 groups with regard to their main known function involving cell structure (24.65%), translation and transcription (12.32%), metabolism and energy production (12.32%), protein synthesis (9.60%), membrane protein receptors or cell signaling (8.21%), cell defense (5.47%), transport (5.47%), cell division (8.21%), vitellogenin (2.73%), unclassified (6.84%) and unknown function (4.10%). The results of this study provide a valuable resource for molecular analysis of normal and abnormal conditions affecting female reproduction. Moreover, it may help to better understand factors affecting caviar quality during refrigerated storage.
Keyvanshokooh
Saeed
S
Department of Fisheries, College of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran.
Vaziri
Behrouz
B
D000818:Animals; D004527:Egg Proteins / Q000737:chemistry / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism; D004798:Enzymes / Q000737:chemistry / Q000302:isolation & purification; D005260:Female; D005399:Fishes / Q000235:genetics*; D010060:Ovulation; D010063:Ovum / Q000502:physiology; D010449:Peptide Mapping; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D020543:Proteome / Q000235:genetics*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin; D014819:Vitellogenins / Q000737:chemistry / Q000302:isolation & purification
D004527:Egg Proteins; D004798:Enzymes; D011506:Proteins; D020543:Proteome; D014819:Vitellogenins; D014357:Trypsin