[Measurement of glutamine concentration in Zelanian rabbits' skeletal muscle by non-invasive magnetic resonance spectroscopy].
OBJECTIVE
To study the feasibility of non-invasive magnetic resonance spectroscopy (MRS) in measuring glutamine (GLN) level in Zelanian rabbits' skeletal muscle.


METHODS
Non-invasive MRS was used to get the data of peak height ratio of GLN + glutamic acid (GLx) at 3.8 ppm and creatine (Cr) at 3.0 ppm, peak area ratio of GLx at 3.8 ppm and Cr at 3.0 ppm. High performance liquid chromatography (HPLC) was used to examine the actual GLx levels of muscle from 22 Zelanian rabbits. The feasibility of MRS was then evaluated by HPLC method.


RESULTS
The ratio of peak height and peak area of GLx and Cr by means of MRS were 0.162 +/- 0.045 and 0.092 +/- 0.065, respectively. The average concentration of GLx in skeletal muscle by means of HPLC was (4.19 +/- 2.50) micromol/g. The ratio of GLx and plasma Cr level by means of HPLC was 4.576 -/+ 0.599. The ratio of peak height and peak area of GLx and Cr by means of MRS were correlated significantly with the ratio of concentration of GLx in skeletal muscle and plasma Cr by means of HPLC (r = 0.7, P = 0.001; r = 0.6, P = 0.001).


CONCLUSIONS
Non-invasive MRS is feasible to measure GLN level in skeletal muscle of rabbit.
Department of General Surgery, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Yue-ping
YP
Fan
Jian-chun
JC
Yu
Fan
F
Zhang
Gui-zhen
GZ
He
Feng
F
Feng
Xiao-zhen
XZ
Li
Zheng-yu
ZY
Jin
D000818:Animals; D005240:Feasibility Studies; D005973:Glutamine / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D018482:Muscle, Skeletal / Q000378:metabolism*; D011817:Rabbits
D005973:Glutamine