Comparison of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR assay to culture by use of BBL CHROMagar MRSA for detection of MRSA in nasal surveillance cultures from an at-risk community population.
We compared the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR assay to culture with BBL CHROMagar MRSA for nasal surveillance among 602 arrestees from the Baltimore City Jail. The sensitivity and specificity were 88.5% and 91.0%, respectively, and after secondary analysis using enrichment broth, they were 89.0% and 91.7%, respectively. Twenty-three of 42 false-positive PCR lysates contained methicillin-susceptible S. aureus.
Farley
Jason E
JE
Johns Hopkins University, School of Nursing, 600 N. Wolfe Street, Carnegie 346, Baltimore, MD 21287, USA. jfarley@son.jhmi.edu
Stamper
Paul D
PD
Ross
Tracy
T
Cai
Mian
M
Speser
Sharon
S
Carroll
Karen C
KC
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques / Q000379:methods*; D015142:Baltimore; D002353:Carrier State / Q000175:diagnosis* / Q000382:microbiology; D003470:Culture Media / Q000737:chemistry; D005189:False Positive Reactions; D006801:Humans; D008297:Male; D016106:Methicillin Resistance; D008875:Middle Aged; D009666:Nose / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011330:Prisons; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*
D003470:Culture Media