Identification of iron-regulated cellular proteins, Fe3+-reducing and -chelating compounds, in the white-rot fungus Perenniporia medulla-panis.
In this paper, we present the responses of the white-rot fungus Perenniporia medulla-panis to iron availability with regard to alterations in growth, expression of cellular proteins, Fe3+-reducing activity, and Fe3+ chelators production. Iron supplementation stimulated fungal growth but did not result in a significant increase in biomass production. Catechol and hydroxamate derivatives were produced mainly under iron deficiency, and their productions were repressed under iron supplementation conditions. Perenniporia medulla-panis showed several cellular proteins in the range of 10-90 kDa. Some of them showed negative iron-regulation. Iron-supplemented medium also repressed both cell surface and extracellular Fe3+-reducing activities; however, the highest cell surface activity was detected at the initial growth phase, whereas extracellular activity increased throughout the incubation period. No significant production of chelators and extracellular Fe3+-reducing activity were observed within the initial growth phase, suggesting that the reduction of Fe3+ to Fe2+ is performed by ferrireductases.
Department of Biotechnology, Escola de Engenharia de Lorena, University of São Paulo - USP Lorena, Estrada Municipal do Campinho, s/no. - Cx Postal 116, 12602-810 Lorena/SP, Brazil. arantes@debiq.eel.usp.br
Valdeir
V
Arantes
Adriane M F
AM
Milagres
D038181:FMN Reductase / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry / Q000378:metabolism*; D005296:Ferrous Compounds / Q000737:chemistry / Q000378:metabolism; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000378:metabolism; D010084:Oxidation-Reduction; D011124:Polyporaceae / Q000201:enzymology* / Q000254:growth & development* / Q000378:metabolism
D005290:Ferric Compounds; D005296:Ferrous Compounds; D005656:Fungal Proteins; D007502:Iron Chelating Agents; D007501:Iron; D038181:FMN Reductase; C023903:ferric citrate iron reductase