Optimization of sterilization of Escherichia coli in milk by surfactin and fengycin using a response surface method.
In this paper, the sensitivity of Escherichia coli to surfactin and fengycin was observed, and the optimization of the antimicrobial activity of surfactin and fengycin to E. coli in milk by a response surface methodology was studied. Results showed that E. coli had high sensitivity to these antibiotics, whose minimal inhibitory concentrations were 15.625 microg.mL(-1) and 31.25 microg.mL(-1), respectively. The optimization result indicated that E. coli could be sterilized by 5 orders of magnitude when the temperature was 5.5 degrees C, the action time was 15.8 h, and the concentration (surfactin/fengycin weight ratio 1:1) was 14.63 microg.mL(-1).
Huang
Xianqing
X
College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, People's Republic of China.
Wei
Zhanyong
Z
Zhao
Gaiming
G
Gao
Xiaoping
X
Yang
Shujing
S
Cui
Yanhong
Y
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D003080:Cold Temperature; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000187:drug effects*; D055666:Lipopeptides; D008074:Lipoproteins / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000382:microbiology*; D010456:Peptides, Cyclic / Q000494:pharmacology*; D013242:Sterilization / Q000379:methods*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D055666:Lipopeptides; D008074:Lipoproteins; D010456:Peptides, Cyclic; C049972:fengycin; C009365:surfactin peptide