DNA ploidy and cell cycle analyses in the bone marrow cells of patients with megaloblastic anemia using laser scanning cytometry.
BACKGROUND
Megaloblastic anemias are characterized by several hematopoietic cells with dysplastic nuclear morphology. The analyses of DNA ploidy and cell cycle of these cells are important to understand the property of such diseases.


METHODS
As laser scanning cytometry (LSC) is a useful tool to evaluate the morphology of the cells fixed on the slide glass together with the quantitative analysis of the fluorescence information of each cell by rapid scanning of the specimens, the authors examined the DNA ploidy and cell cycle of six cases with megaloblastic anemia using LSC.


RESULTS
Giant neutrophilic series such as giant metamyelocytes and giant band cells were found to have extraordinarily higher DNA ploidy, while hypersegmented neutrophils represented the normal diploid pattern like normal neutrophils. As to megaloblasts, cell cycle analysis showed that the proportion of the cells in S phase was increased as compared with the case of normal erythroblasts.


CONCLUSIONS
The present study clearly demonstrates the abnormal aspects of the hematopoietic cells with megaloblastic anemia from the viewpoint of the DNA ploidy and cell cycle analyzed by the use of LSC.
Tsujioka
Takayuki
T
Department of Laboratory Medicine, Kawasaki Medical School, Okayama 701-0192, Japan.
Tochigi
Aki
A
Kishimoto
Mitsuyo
M
Kondo
Toshinori
T
Tasaka
Taizo
T
Wada
Hideho
H
Sugihara
Takashi
T
Yoshida
Yataro
Y
Tohyama
Kaoru
K
D000749:Anemia, Megaloblastic / Q000097:blood / Q000175:diagnosis* / Q000235:genetics*; D001854:Bone Marrow Cells / Q000473:pathology*; D002453:Cell Cycle / Q000235:genetics*; D002467:Cell Nucleus / Q000473:pathology; D002869:Chromosome Aberrations; D004247:DNA / Q000235:genetics; D015726:Giant Cells / Q000473:pathology; D042381:Granulocyte Precursor Cells / Q000473:pathology; D018922:HL-60 Cells; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006801:Humans; D047569:Laser Scanning Cytometry / Q000379:methods*; D008534:Megaloblasts / Q000473:pathology; D009504:Neutrophils / Q000473:pathology; D011003:Ploidies; D016196:S Phase / Q000235:genetics
D004247:DNA