In vitro evaluation of platelet concentrates during storage: Platelet counts and markers of platelet destruction.
BACKGROUND
Differences in platelet counts are observed by use of automated haematology analyzers making interlaboratory comparison difficult.


METHODS
Twenty-eight single-donor platelet concentrates (PCs) were collected. Platelet concentration and markers of platelet destruction were investigated during storage for 11/12 days.


RESULTS
Increasing impedance-immunoplatelet ratio was observed during storage, correlating to platelet fragments, large platelets, platelet density and cell-lysis. High variability was observed for optical-immunoplatelet ratio.


CONCLUSIONS
Immunoplatelet count or correction factor calculated by impedance-immunoplatelet ratio should be used to confirm that platelet unit meets platelet count requirements or to compare results from clinical trials. Optical platelet count is not recommended.
Blood Bank, Haukeland University Hospital, N-5021 Bergen, Norway. torunn.oveland.apelseth@helse-bergen.no
Torunn O
TO
Apelseth
Tor
T
Hervig
D001792:Blood Platelets / Q000166:cytology*; D017097:Electric Impedance; D006801:Humans; D010976:Platelet Count / Q000379:methods*; D011309:Preservation, Biological; D013997:Time Factors