Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source.
We present a time-gated, optically sectioned, hyperspectral fluorescence lifetime imaging (FLIM) microscope incorporating a tunable supercontinuum excitation source extending into the UV. The system is capable of resolving the excitation spectrum, emission spectrum, and fluorescence decays in an optically sectioned image.
Chemical Biology Centre, Photonics Group, Department of Physics, Imperial College London, UK. dylan.owen00@imperial.ac.uk
Dylan M
DM
Owen
Egidijus
E
Auksorius
Hugh B
HB
Manning
Clifford B
CB
Talbot
Pieter A A
PA
de Beule
Christopher
C
Dunsby
Mark A A
MA
Neil
Paul M W
PM
French
D000818:Animals; D003094:Collagen / Q000737:chemistry / Q000648:ultrastructure; D031405:Convallaria / Q000166:cytology; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D008860:Microscopy, Ultraviolet / Q000295:instrumentation / Q000379:methods; D051381:Rats; D013623:Tail / Q000166:cytology
D003094:Collagen