Functional grouping of osteoclast genes revealed through microarray analysis.
We describe for the first time functional clusters of genes that are modulated during the differentiation of osteoclasts. Pathway analysis was applied to gene array data generated from affymetrix chips hybridized to RNA isolated from RAW264.7 cells exposed to RANK-ligand (RANK-L) for 5 days. This analysis revealed major functional gene clusters that were either up- or down-regulated during osteoclastogenesis. Some of the genes within the clusters have known functions, while others do not. We discuss herein the relevance of these functional gene clusters and their modulation to biological processes underlying the formation, function, and fate of osteoclasts.
Yang
Guozhe
G
Mount Sinai Bone Program, Mount Sinai School of Medicine, New York, NY 10029, USA.
Zaidi
Mone
M
Zhang
Weijia
W
Zhu
Ling-Ling
LL
Li
Jianhua
J
Iqbal
Jameel
J
Varbanov
Alex
A
Gross
Gary
G
Phipps
Roger
R
Troen
Bruce R
BR
Sun
Li
L
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D051379:Mice; D005810:Multigene Family / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D010010:Osteoclasts / Q000166:cytology* / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*
D020543:Proteome