Bacterial surface display of GFP(uv) on bacillus subtilis spores.
To analyze a cotG-based Bacillus subtilis spore display system directly, GFP(uv) was expressed on the surface of Bacillus subtilis spores. When GFP(uv) was fused to the C-terminal of the cotG structural gene and expressed, the existence of a CotG-GFP(uv) fusion protein on the B. subtilis spore was confirmed by flow cytometry confocal microscopic analysis. When the cotG anchoring motif was deleted, no fluorescence emission was observed under flow cytometry and confocal microscopic analysis from the purified spore, confirming the essential role of CotG as an anchoring motif. This GFP(uv) displaying spore might be used for another signaling application triggered by intracellular or extracellular stimuli.
Kim
June-Hyung
JH
Department of Chemical Engineering, Dong-A University, Busan 604-714, Korea.
Roh
Changhyun
C
Lee
Chang-Won
CW
Kyung
Dohyun
D
Choi
Soo-Keun
SK
Jung
Heung-Chae
HC
Pan
Jae-Gu
JG
Kim
Byung-Gee
BG
D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D013171:Spores, Bacterial / Q000235:genetics
D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; C091352:cotG protein, Bacillus subtilis; D049452:Green Fluorescent Proteins