Marked correlations in protein expression identified by proteomic analysis of human spermatozoa.
The present work was started to explore whether a correlation could be detected among proteomic expression, protamine content and DNA integrity in human sperm cells. Towards this goal, we extracted the proteins present in the sperm cells from 47 sperm samples from infertile patients and from ten semen donors, analysed each sample by 2-D gel electrophoresis, and quantified the expression of 101 spots identified by MALDI-TOF analysis. Additionally, the protamine content and DNA integrity were also determined. Several interesting proteins such as transcription factors, prohibitin, heat shock and proteasome proteins have been identified. We have found that the expression of an important number of proteins (58 different 2-D spots) is correlated in independent sperm samples at high statistical significance (p<0.001 and r>0.5). Additionally, eight proteins have also been found to correlate with DNA integrity and seven with protamine content (p<0.05). To our knowledge, this is the first report describing the correlation between proteomics, DNA integrity and protamine content. It also sheds new light into the fundamental aspects of the human sperm and points to new potential proteins involved in male infertility.
de Mateo
Sara
S
Human Genetics Research Group, IDIBAPS, Faculty of Medicine, University of Barcelona, Barcelona, Spain.
Martínez-Heredia
Juan
J
Estanyol
Josep Maria
JM
Domínguez-Fandos
David
D
Domíguez-Fandos
David
D
Vidal-Taboada
José Manuel
JM
Ballescà
José Luis
JL
Oliva
Rafael
R
D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression; D006801:Humans; D020287:In Situ Nick-End Labeling; D007248:Infertility, Male / Q000235:genetics / Q000378:metabolism*; D008297:Male; D000091402:Prohibitins; D011479:Protamines / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013094:Spermatozoa / Q000737:chemistry* / Q000378:metabolism
C082073:PRM1 protein, human; D000091402:Prohibitins; D011479:Protamines; D011506:Proteins; D020543:Proteome; D012097:Repressor Proteins; C068621:protamine 2