Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source.
We present a time-gated, optically sectioned, hyperspectral fluorescence lifetime imaging (FLIM) microscope incorporating a tunable supercontinuum excitation source extending into the UV. The system is capable of resolving the excitation spectrum, emission spectrum, and fluorescence decays in an optically sectioned image.
Owen
Dylan M
DM
Chemical Biology Centre, Photonics Group, Department of Physics, Imperial College London, UK. dylan.owen00@imperial.ac.uk
Auksorius
Egidijus
E
Manning
Hugh B
HB
Talbot
Clifford B
CB
de Beule
Pieter A A
PA
Dunsby
Christopher
C
Neil
Mark A A
MA
French
Paul M W
PM
D000818:Animals; D003094:Collagen / Q000737:chemistry / Q000648:ultrastructure; D031405:Convallaria / Q000166:cytology; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D008860:Microscopy, Ultraviolet / Q000295:instrumentation / Q000379:methods; D051381:Rats; D013623:Tail / Q000166:cytology
D003094:Collagen