Production of monoclonal antibody against Listeria monocytogenes and its application to immunochromatography strip test.
An immunochromatography (ICG) strip test based on a monoclonal antibody for the rapid detection of L. monocytogenes in meat and processed-meat samples was developed in this study. A monoclonal antibody (MAb) specific to L. monocytogenes was produced from cloned hybridoma cells (FKLM-3B12-37) and used to develop an ICG strip test. The antibody showed a stronger binding to L. monocytogenes than other Listeria species, and a weak cross-reaction to S. aureus based on an ELISA. The detection limit of the ICG strip test was 10(5) cell/ml. In total, 116 meat and processed-meat samples were collected and analyzed using both the ICG strip test and a PCR. The ICG strip test and PCR indicated L. monocytogenes contamination in 34 and 27 meat samples, respectively. The 7 meat samples not identified as L. monocytogenes positive by the PCR were also tested using an API kit and found to be contaminated by Listeria species. In conclusion, the ICG strip test results agreed well with those obtained using the PCR and API kit. Thus, the developed ICG has potential use as a primary screening tool for L. monocytogenes in various foods and agricultural products, generating results within 20 min without complicated steps.
Shim
Won-Bo
WB
Division of Applied Life Science (Brain Korea 21 Program), Graduate School of Gyeongsang National University, Chinju 660-701, Korea.
Choi
Jin-Gil
JG
Kim
Ji-Young
JY
Yang
Zheng-You
ZY
Lee
Kyu-Ho
KH
Kim
Min-Gon
MG
Ha
Sang-Do
SD
Kim
Keun-Sung
KS
Kim
Kwang-Yup
KY
Kim
Cheol-Ho
CH
Ha
Kwang-Soo
KS
Eremin
Sergei A
SA
Chung
Duck-Hwa
DH
D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002845:Chromatography / Q000379:methods*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D017979:Gold Colloid / Q000378:metabolism; D007118:Immunoassay / Q000379:methods*; D008089:Listeria monocytogenes / Q000276:immunology*; D015203:Reproducibility of Results; D013211:Staphylococcus aureus / Q000276:immunology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D017979:Gold Colloid