Anti-inflammatory effects of recombinant arginine deiminase originating from Lactococcus lactis ssp. lactis ATCC 7962.
Arginine deiminase (ADI, E.C. 3.5.3.6), one of the arginine deprivation enzymes, exhibits anticarcinogenic activities. The present study investigated the anti-inflammatory activities of the purified recombinant ADI originating from Lactococcus lactis ssp. lactis ATCC7962 (LADI). LADI dose-dependently inhibited lipopolysaccharide (LPS)-induced upregulation of inducible nitric oxide synthase and the production of nitric oxide in RAW 264.7 murine macrophages. The induction of cyclooxygenase-2 expression and subsequent production of prostaglandin E2 by LPS was also attenuated by LADI treatment. Moreover, LADI inhibited the production of interleukin-6 in LPS-stimulated RAW 264.7 macrophages. These results indicate that LADI exerts anti-inflammatory effects, which may in part explain its chemopreventive potential.
Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea.
Jong-Eun
JE
Kim
Haeng Jeon
HJ
Hur
Ki Won
KW
Lee
Hyong Joo
HJ
Lee
D000975:Antioxidants / Q000494:pharmacology; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006867:Hydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013294:Lactococcus lactis / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000201:enzymology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
D000975:Antioxidants; D011994:Recombinant Proteins; D019001:Nitric Oxide Synthase; D051546:Cyclooxygenase 2; D006867:Hydrolases; C019723:arginine deiminase