Fragment responsible for translocation in the N-terminal domain of human topoisomerase I.
The N-terminal domain is a fragment that binds proteins and anchors topoisomerase I in the nucleolus. As a separate polypeptide, it translocates from the nucleolus to nucleoplasm upon camptothecin treatment. In this paper, we show that the translocation depends on the short fragment of the domain (residues from 1 to 67). We also present a list of proteins that specifically bind to the fragment responsible for translocation.
Girstun
Agnieszka
A
Institute of Biochemistry, Faculty of Biology, University of Warsaw, ul. Miecznikowa 1, 02-096 Warszawa, Poland.
Kowalska-Loth
Barbara
B
Czubaty
Alicja
A
Klocek
Magdalena
M
Staroń
Krzysztof
K
D000595:Amino Acid Sequence; D004264:DNA Topoisomerases, Type I / Q000737:chemistry* / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport
D010446:Peptide Fragments; D004264:DNA Topoisomerases, Type I