Fluorescence resonance energy transfer in ferritin labeled with multiple fluorescent dyes.
We simultaneously labeled ferritin with two Alexa Fluor fluorophores (AF350 and AF430). When both fluorophores label the same ferritin subunit, fluorescence resonance energy transfer (FRET) takes place from the excited AF350 to the acceptor AF430. By varying the number and the ratio of labeling fluorophores, we can modulate FRET such that the ferritin particles can exhibit multiple colors under UV illumination. Labeling of the ferritin shell does not affect the properties of the metallic core.
Departamento de Química Inorgánica, Facutad de Ciencias, Universidad de Granada, 18071, Granada, Spain.
Belén
B
Fernández
Natividad
N
Gálvez
Purificación
P
Sánchez
Rafael
R
Cuesta
Ruperto
R
Bermejo
José M
JM
Domínguez-Vera
D000818:Animals; D005293:Ferritins / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006736:Horses
D005456:Fluorescent Dyes; D005293:Ferritins