Citrus auraptene acts as an agonist for PPARs and enhances adiponectin production and MCP-1 reduction in 3T3-L1 adipocytes.
Citrus fruit compounds have many health-enhancing effects. In this study, using a luciferase ligand assay system, we showed that citrus auraptene activates peroxisome proliferator-activated receptor (PPAR)-alpha and PPARgamma. Auraptene induced up-regulation of adiponectin expression and increased the ratio of the amount of high-molecular-weight multimers of adiponectin to the total adiponectin. In contrast, auraptene suppressed monocyte chemoattractant protein (MCP)-1 expression in 3T3-L1 adipocytes. Experiments using PPARgamma antagonist demonstrated that these effects on regulation of adiponectin and MCP-1 expression were caused by PPARgamma activations. The results indicate that auraptene activates PPARgamma in adipocytes to control adipocytekines such as adiponectin and MCP-1 and suggest that the consumption of citrus fruits, which contain auraptene can lead to a partial prevention of lipid and glucose metabolism abnormalities.
Kuroyanagi
Kayo
K
Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
Kang
Min-Sook
MS
Goto
Tsuyoshi
T
Hirai
Shizuka
S
Ohyama
Kana
K
Kusudo
Tatsuya
T
Yu
Rina
R
Yano
Masamichi
M
Sasaki
Takao
T
Takahashi
Nobuyuki
N
Kawada
Teruo
T
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D018932:Chemokine CCL2 / Q000378:metabolism*; D002957:Citrus / Q000378:metabolism*; D003374:Coumarins / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D051379:Mice; D047492:Peroxisome Proliferator-Activated Receptors / Q000819:agonists*; D010936:Plant Extracts / Q000008:administration & dosage*
D018932:Chemokine CCL2; D003374:Coumarins; D047492:Peroxisome Proliferator-Activated Receptors; D010936:Plant Extracts; C105832:aurapten