Multiplex-PCR assay for identification of Klebsiella pneumoniae isolates carrying the cps loci for K1 and K2 capsule biosynthesis.
Multiplex-PCR assay for identification of Klebsiella pneumoniae isolates carrying gene clusters for biosynthesis of capsular polysaccharide (CPS) types K1 and K2 was developed. Genes wzc and orf10 of the cps cluster were applied as K1 and K2 specific markers respectively. The assay specificity was confirmed using 147 isolates of Klebsiella spp. including 77 K-antigen reference strains. The multiplex-PCR assay was found simple and cost-effective tool for identification of K. pneumoniae clinical isolates of K1 and K2 geno-serotypes.
Department of Bacteriology, National Institute of Hygiene, Warsaw, Poland. rgierczynski@pzh.gov.pl
Rafał
R
Gierczyński
Marek
M
Jagielski
Waldemar
W
Rastawicki
Stanisław
S
Kałuzewski
D000942:Antigens, Bacterial / Q000096:biosynthesis*; D016667:Bacterial Capsules; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*
D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial; C036962:capsular polysaccharide K1; C068924:capsular polysaccharide K2