Properties of flaky affinity resin with co-continuous structure.
A new type of flaky affinity resin for capture of the target proteins was prepared to discuss its properties compared with those of a particulate affinity resin. The resin prepared had totally co-continuous structure (monolith) and was utilized in the shape of flake. The concentration of surface amino groups for immobilization of ligand was determined to be 22.3 micromol/ml. Immobilizations of ligand such as Sulfonamide, Ketoprofen, Captopril, or Methotrexate (MTX) on the affinity resin were quantitatively proceeded to afford fully covered (100%) affinity resins. Control of the immobilization rate of affinity resin using Sulfonamide or Ketoprofen was successfully achieved with the calculated immobilization rate. The flaked shape of affinity resin (100-400 microm) presumably simplified affinity experimental procedures and the affinity resin immobilizing Sulfonamide effectively captured one of the target proteins, CAII, without non-specifically bound proteins. The observed properties of the flaky affinity resin as well as ease in handling are really useful for capture of the target proteins of possible rare ligands.
Mori
Tomoko
T
Graduate School of Environmental Studies, Tohoku University, Aoba 6-6-20, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
Tanaka
Akito
A
Kubo
Takuya
T
Kaya
Kunimitsu
K
Sakamoto
Mari
M
Hosoya
Ken
K
D000588:Amines; D002846:Chromatography, Affinity / Q000379:methods*; D008024:Ligands; D008722:Methods; D011506:Proteins / Q000302:isolation & purification*; D012117:Resins, Synthetic / Q000737:chemistry*; D013499:Surface Properties
D000588:Amines; D008024:Ligands; D011506:Proteins; D012117:Resins, Synthetic