Immunohistochemical analysis of hepatic ganglioside distribution following a partial hepatectomy and exposure to different hyperbaric oxygen treatments.
Ganglioside GM3(Neu5Ac) expression is highly increased in liver 54h following 15% partial hepatectomy in pre-operatively oxygenated rats. GM3(Neu5Gc), GM2, GalNAc-GM1b and gangliosides of the neolacto-series are less affected. GM3(Neu5Ac) is a potent inhibitor of epidermal growth factor signaling. Since GM3(Neu5Ac) growth inhibitory effect depends on its cellular localization, the aim of this study was to detect ganglioside cellular localization during liver regeneration. The experiment was performed using the same rat model which previously showed increased ganglioside expression and more efficient liver regeneration. Frozen sections of liver were analyzed using confocal microscopy after labeling for binding of five ganglioside-specific antibodies, with or without hepatocyte membrane permeabilization. Ganglioside precursors, ceramide (Cer), monohexaosylceramide and lactosylceramide (LacCer) were determined by high-performance thin-layer chromatography. Apoptosis was assessed by fluorescein-dUTP end-labeling of fragmented DNA. Liver of pre-operative oxygenated rats showed high perinuclear labeling of GM3(Neu5Ac) which was absent in post-operative oxygenated and control animals. In the same group, Cer content was lower, monohexaosylceramide and LacCer were absent, and content of apoptotic cells was significantly the lowest, compared to other groups examined (F=20.36, p=0.0001). These findings indicate that ganglioside GM3(Neu5Ac) may be involved in mediation of beneficial effects of pre-operatively oxygenation during the liver regeneration.
Department of Pathophysiology, Split University Hospital, Soltanska 2, 21000 Split, Croatia.
T
T
Ticinović-Kurir
V
V
Cikes-Culić
T
T
Zemunik
I
I
Grković
J
J
Terzić
M
M
Padovan
N M
NM
Petri
A
A
Markotić
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002518:Ceramides / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005732:Gangliosides / Q000032:analysis*; D006498:Hepatectomy / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D008115:Liver Regeneration / Q000187:drug effects; D018613:Microscopy, Confocal; D010100:Oxygen / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D002518:Ceramides; D005732:Gangliosides; D010100:Oxygen