Detection of unusual rotavirus genotypes G8P[8] and G12P[6] in South Korea.
Five hundred four fecal specimens, collected between 2004 and 2006 from young children with acute diarrhea, were screened for rotavirus by ELISA with VP6-specific antibody. Of these samples, 394 (78.2%) were confirmed as group A rotavirus and they underwent G- and P typing using a combination of ELISA, RT-PCR, and sequence analysis methods. The dominant circulating G serotype was G1 (35.6%) followed by G3 (26.4%), G4 (14.7%), and G2 (11.9%). There was a low prevalence of G9 (1.0%) and of unusual G type rotavirus, in particular, G12 (0.5%) and G8 (0.3%). Of the P genotype rotavirus in circulation, P[8] (53.0%) was most common followed by P[6] (15.5%), P[4] (15.2%), and P[9] (2.3%). Determination of G- and P type combinations revealed that G1P[8] strains were most prevalent (25.4%), amid G3P[8] (16.8%), G2P[4] (6.3%), and G4P[6] (6.1%) strains. Unusual or rare combinations such as G2P[6], G2P[8], G3P[4], G2P[9], G1P[9], G3P[9], G12P[6], G1P[4], G3P[6], and G8P[8] were also found. Owing to the recent emergence of G8 and G12 rotavirus, the findings from this study are important since they provide new information concerning the local and global spread of rotavirus genotypes.
Le
Van Phan
VP
Department of Microbiology, Chung-Ang University College of Medicine, Seoul, South Korea.
Kim
Ji-Yeon
JY
Cho
Sung-Lim
SL
Nam
Sun-Woo
SW
Lim
Inseok
I
Lee
Hee-Joong
HJ
Kim
Kijeong
K
Chung
Sang-In
SI
Song
Wonkeun
W
Lee
Kyu Man
KM
Rhee
Moon-Soo
MS
Lee
Jung-Sook
JS
Kim
Wonyong
W
D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D036022:Capsid Proteins / Q000276:immunology*; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology; D005838:Genotype; D006801:Humans; D007223:Infant; D007723:Korea / Q000453:epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology
D000956:Antigens, Viral; D036022:Capsid Proteins; C061332:VP6 protein, Rotavirus