Hyaluronate-covered nanoparticles for the therapeutic targeting of cartilage.
Hyaluronic acid (HA) has a high affinity for the CD44 receptor present at the surface of articular cells, particularly of chondrocytes. HA-covered polylactide nanoparticles containing bioactive compounds such as HA and chondroitin sulfate (CS) were thus prepared in order to achieve a controlled delivery targeted to cartilage cells after injection near articular alterations/erosions. Such nanoparticles (diameter = 700 nm) were prepared by double emulsion/solvent evaporation, using amphiphilic derivatives of HA, as stabilizer of the secondary emulsion. These nanoparticles were incubated with articular cells, and several tests were carried out. First, they proved that the nanospheres provoked no decrease in cell viability, even after 72 h of contact. Second, a confocal microscopy analysis on fluorescent HA-covered particles showed that they were captured by articular cells, while with those covered with poly(vinyl alcohol), the uptake was far lower. Third, a scattering electron microscopy analysis proved that the HA-coated nanoparticles were localized in the cell intracytoplasmic area.
Laboratoire de Chimie Physique Macromoléculaire, UMR CNRS-INPL 7568, BP 20451, 54001 Nancy Cedex, France.
Hamed
H
Laroui
Laurent
L
Grossin
Michèle
M
Léonard
Jean-François
JF
Stoltz
Pierre
P
Gillet
Patrick
P
Netter
Edith
E
Dellacherie
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects; D002417:Cattle; D016503:Drug Delivery Systems / Q000379:methods; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000737:chemistry*; D008297:Male; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D006820:Hyaluronic Acid