Increasing genomic information in bivalves through new EST collections in four species: development of new genetic markers for environmental studies and genome evolution.
The generation of EST information is an essential step in the genomic characterisation of species. In the context of the European Network Marine Genomics, a common goal was to significantly increase the amount of ESTs in commercial marine mollusk species and more specifically in the less studied but ecologically and commercially important groups, such as mussel and clam genera. Normalized cDNA libraries were constructed for four different relevant bivalves species (Crassostrea gigas, Mytilus edulis, Ruditapes decussatus and Bathymodiolus azoricus), using numerous tissues and physiological conditions. In this paper, we present the analysis of the 13,013 expressed sequence tags (ESTs) generated. Each EST library was independently assembled and 1300-3000 unique sequences were identified in each species. For the different species, functional categories could be assigned to only about 16 to 27% of ESTs using the GO annotation tool. All sequences have been incorporated into a publicly available database and form the basis for subsequent microarray design, SNP detection and polymorphism analysis, and the placement of novel markers on genetic linkage maps.
UMR CNRS 7144 UPMC Evolution et Génétique des Populations Marines, Station Biologique, Université Européenne de Bretagne, BP 74, Place Georges Teissier, 29682, Roscoff, France. atanguy@sb-roscoff.fr
Arnaud
A
Tanguy
Nicolas
N
Bierne
Carlos
C
Saavedra
Benjamin
B
Pina
Evelyne
E
Bachère
Michael
M
Kube
Eric
E
Bazin
François
F
Bonhomme
Pierre
P
Boudry
Viviane
V
Boulo
Isabelle
I
Boutet
Leonor
L
Cancela
Carole
C
Dossat
Pascal
P
Favrel
Arnaud
A
Huvet
Sergio
S
Jarque
Didier
D
Jollivet
Sven
S
Klages
Sylvie
S
Lapègue
Ricardo
R
Leite
Jeanne
J
Moal
Dario
D
Moraga
Richard
R
Reinhardt
Jean-François
JF
Samain
Eleftherios
E
Zouros
Adelino
A
Canario
D000818:Animals; D049872:Bivalvia / Q000235:genetics* / Q000502:physiology; D004777:Environment; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D015723:Gene Library; D005819:Genetic Markers; D016678:Genome; D023281:Genomics; D018895:Microsatellite Repeats; D020641:Polymorphism, Single Nucleotide; D020080:Tandem Repeat Sequences
D005819:Genetic Markers