Monoclonal antibody proteomics: discovery and prevalidation of chronic obstructive pulmonary disease biomarkers in a single step.
We define mAb proteomics as the global generation of disease specific antibodies that permit mass screening of biomarkers. An integrated, high-throughput, disease-specific mAb-based biomarker discovery platform has been developed. The approach readily provided new biomarker leads with the focus on large-scale discovery and production of mAb-based, disease-specific clinical assay candidates. The outcome of the biomarker discovery process was a highly specific and sensitive assay, applicable for testing of clinical validation paradigms, like response to treatment or correlation with other clinical parameters. In contrast to MS-based or systems biology-based strategies, our process produced prevalidated clinical assays as the outcome of the discovery process. By re-engineering the biomarker discovery paradigm, the encouraging results presented in this paper clearly demonstrate the efficiency of the mAb proteomics approach, and set the grounds for the next steps of studies, namely, the hunt for candidate biomarkers that respond to drug treatment.
Department of Pulmonology, University of Debrecen, Debrecen, Hungary.
Eszter
E
Csanky
Petra
P
Olivova
Eva
E
Rajnavolgyi
William
W
Hempel
Nadege
N
Tardieu
Elesne Toth
ET
Katalin
Anne
A
Jullien
Carole
C
Malderez-Bloes
Mariana
M
Kuras
Manuel X
MX
Duval
Laszlo
L
Nagy
Beata
B
Scholtz
William
W
Hancock
Barry
B
Karger
András
A
Guttman
Laszlo
L
Takacs
D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification*; D000970:Antineoplastic Agents; D015415:Biomarkers / Q000032:analysis; D001798:Blood Proteins; D002851:Chromatography, High Pressure Liquid; D019295:Computational Biology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods* / Q000639:trends; D040081:Protein Array Analysis; D029424:Pulmonary Disease, Chronic Obstructive / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D015415:Biomarkers; D001798:Blood Proteins