Combining array-based approaches for the identification of candidate tumor suppressor loci in mature lymphoid neoplasms.
Tumor suppressor gene (TSG) inactivation by chromosomal deletions and/or mutations is a well-characterized genetic alteration in lymphomas. Array-based technologies have greatly increased the detection and characterization of chromosomal imbalances and regions with loss of heterozygosity (LOH), leading to the identification of a number of novel candidate TSG loci. In addition, microarray platforms for studying DNA methylation and histone modifications enable identifying epigenetic changes affecting gene expression of TSG. Combining these microarray technologies with gene expression profiling is a promising strategy to discover novel TSG in regions targeted by genetic or epigenetic alterations. In this review we present an outline of methodological aspects of the various microarray technologies, and discuss their potentials and restrictions. Furthermore, we survey research findings derived from these high-throughput techniques, which are allowing a deeper insight into the mechanisms of lymphomagenesis.
Institute of Human Genetics, University Hospital Schleswig-Holstein, Campus Kiel, Christian-Albrechts University, Kiel, Germany.
Inga
I
Nieländer
Stefanie
S
Bug
Julia
J
Richter
Maciej
M
Giefing
José Ignacio
JI
Martín-Subero
Reiner
R
Siebert
D002872:Chromosome Deletion; D002874:Chromosome Mapping; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity; D008223:Lymphoma / Q000145:classification / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis