[Biochemical and photometric studies of modification of collagen structure under UV radiation].
The influence of UV irradiation (270-380 nm) on the biochemical, fluorescence and colorimetric properties of collagen was studied. The long-term UV irradiation (120 h) was accompanied by the increase of structural stability of collagen to specific and nonspecific proteolytic enzymes, by formation of new additional fluorophore-containing molecules, by the increase in quantity of carbonyl groups in the collagen and by strong changing of the distribution pattern of alkaline hydrolysis products during gel-chromatography. The coordinates of colour of the collagen films are also changed. These changes of collagen suggest that UV irradiation induces photomodification and photooxidation processes in collagen.
G A
GA
Rebrova
V K
VK
Vasilevskiĭ
L B
LB
Rebrov
L A
LA
Osipova
V A
VA
Bykov
D002850:Chromatography, Gel; D003094:Collagen / Q000528:radiation effects*; D017364:Collagenases / Q000737:chemistry; D008854:Microscopy, Electron; D010084:Oxidation-Reduction; D010434:Pepsin A / Q000737:chemistry; D014466:Ultraviolet Rays
D003094:Collagen; D010434:Pepsin A; D017364:Collagenases