Diverse endophytic nitrogen-fixing bacteria isolated from wild rice Oryza rufipogon and description of Phytobacter diazotrophicus gen. nov. sp. nov.
Twenty-three nitrogen-fixing bacteria were isolated from surface-sterilized stems and roots of wild rice Oryza rufipogon. Four clusters were defined among these bacteria by SDS-PAGE protein patterns and further confirmed by IS-PCR finger-printing analysis. Phylogenetic analysis of 16S rRNA gene sequences showed that the representative strains LS 8 and LS 18 of cluster II formed a monophyletic group sharing 94.0-97.3% similarities with defined enterobacterial species within the genera Salmonella, Citrobacter, Pantoea, Klebsiella, and Enterobacter. DNA-DNA hybridization, physiological, biochemical tests, and cell morphology also revealed that these strains could be differentiated from the related enterobacterial species. Based upon these results, we propose Phytobacter diazotrophicus gen. nov., sp. nov. to the bacterial group represented by strains LS 8 and LS 18. The type strain is LS 8(T) (=DSM 17806(T) = LMG 23328(T) = CGMCC 1.5339(T)). The DNA G+C content of strain LS 8(T) is 58.6 +/- 0.5 mol%.
Zhang
Guo Xia
GX
Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Peng
Gui Xiang
GX
Wang
En Tao
ET
Yan
Hui
H
Yuan
Qing Hua
QH
Zhang
Wu
W
Lou
Xu
X
Wu
Hui
H
Tan
Zhi Yuan
ZY
D001483:Base Sequence; D016172:DNA Fingerprinting; D004591:Electrophoresis, Polyacrylamide Gel; D004755:Enterobacteriaceae / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D012275:Oryza / Q000382:microbiology*; D010088:Oxidoreductases / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; C029130:nitrogenase reductase