Analysis of the stress effects of endocrine disrupting chemicals (EDcs) on Escherichia coli.
In this study, three of the representative EDCs, 17beta-estradiol, bisphenol A, and styrene, were employed to find their mode of toxic actions in E. coli. To accomplish this, four different stress response genes, recA, katG, fabA, and grpE genes, were used as a representative for DNA, oxidative, membrane, or protein damage, respectively. The expression levels of these four genes were quantified using a real-time RT-PCR after challenge with three different EDCs individually. Bisphenol A and styrene caused high-level expression of recA and katG genes, respectively, whereas 17beta-estradiol made no significant changes in expression of any of those genes. These results lead to the classification of the mode of toxic actions of EDCs on E. coli.
College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
Yeon Seok
YS
Kim
Jiho
J
Min
Han Na
HN
Hongz
Ji Hyun
JH
Park
Kyeong Seo
KS
Park
Man Bock
MB
Gu
D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001559:Benzhydryl Compounds; D002374:Catalase / Q000096:biosynthesis; D004926:Escherichia coli / Q000737:chemistry / Q000187:drug effects*; D029968:Escherichia coli Proteins / Q000096:biosynthesis; D004958:Estradiol / Q000633:toxicity*; D054889:Fatty Acid Synthase, Type II; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D006836:Hydro-Lyases / Q000096:biosynthesis; D010636:Phenols / Q000633:toxicity*; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011938:Rec A Recombinases / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020058:Styrene / Q000633:toxicity*; D000098826:Bisphenol A Compounds
D000900:Anti-Bacterial Agents; D001559:Benzhydryl Compounds; D002374:Catalase; D029968:Escherichia coli Proteins; D004958:Estradiol; D054889:Fatty Acid Synthase, Type II; D006360:Heat-Shock Proteins; D006836:Hydro-Lyases; D010636:Phenols; D012329:RNA, Bacterial; D012333:RNA, Messenger; D011938:Rec A Recombinases; D020058:Styrene; C489400:GrpE protein, E coli; C051253:katG protein, E coli; C047118:3-hydroxyacyl-(acyl-carrier-protein) dehydratase; C006780:bisphenol A; D000098826:Bisphenol A Compounds