Reporter gene imaging using radiographic contrast from nonradioactive iodide sequestered by the sodium-iodide symporter.
The hypothesis that the human sodium-iodide symporter, NIS, can be used to detect NIS expression using standard radiological techniques was tested using adenoviral transduced NIS expression in human tumor xenografts grown in mice and in a naive dog prostate. Nonradioactive iodide was administered systemically to animals that 1-3 days previously had received a local injection of a replication-competent adenovirus expressing NIS under the control of the CMV promoter. The distribution of radiopacity was assessed in mouse tumors using micro-CT and a clinical X-ray machine and in the prostate of an anesthetized dog using a clinical spiral CT. Iodide sequestration and NIS expression were measured using X-ray spectrochemical analysis and fluorescence microscopy, respectively. Radiographic contrast due to NIS gene expression that was observed indicates the technique has potential for use in preclinical rodent tumor studies but probably lacks sensitivity for human use.
Brown
Stephen L
SL
Department of Radiation Oncology, Henry Ford Health System, Detroit, MI 48202, USA. sbrown1@hfhs.org
Freytag
Svend O
SO
Barton
Kenneth N
KN
Flynn
Michael J
MJ
Peck
Donald J
DJ
Dragovic
Aleksandar F
AF
Jin
Ryan
R
Yeni
Yener N
YN
Fyhrie
David P
DP
Les
Clifford M
CM
Zhu
Guopei
G
Kolozsvary
Andrew
A
Pitchford
Wayne C
WC
Nathanson
S David
SD
Fenstermacher
Joseph D
JD
Kim
Jae Ho
JH
D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000032:analysis* / Q000737:chemistry; D004285:Dogs; D017930:Genes, Reporter; D006801:Humans; D007455:Iodine / Q000032:analysis* / Q000737:chemistry; D008297:Male; D051379:Mice; D027981:Symporters / Q000235:genetics* / Q000378:metabolism; D014057:Tomography, X-Ray Computed; D000099209:Sodium-Iodide Symporters
D003287:Contrast Media; D007455:Iodine; D027981:Symporters; D000099209:Sodium-Iodide Symporters