Association of MIF-173 gene polymorphism with inflammatory bowel disease in Chinese Han population.
OBJECTIVE
To study whether macrophage migration inhibitory factor (MIF)-173 gene polymorphism correlates with inflammatory bowel disease (IBD) in Chinese Han population.


METHODS
MIF-173 single nucleotide polymorphism (SNP) was genotyped by tetra-primer amplification refractory mutation system (ARMS) and restriction fragment length polymorphisms (RFLP)-PCR in 142 healthy subjects and 98 patients with inflammatory bowel disease (IBD).


RESULTS
There were no discrepancies between the results obtained by tetra-primer ARMS and RFLP-PCR. The frequency of MIF-173 CC genotype was significantly higher in patients with ulcerative colitis (UC) 15.5% than in healthy individuals 5.6% (chi(2)=6.066, P=0.018, OR=3.067 and 95% CI=1.257-7.482). There was a trend towards a higher frequency of CC genotype among CD patients compared with healthy controls, however this did not attain the statistical significance (P=0.245).


CONCLUSIONS
MIF-173 CC genotype may be associated with susceptibility to UC.
Department of Gastroenterology, Zhejiang Provincial People's Hospital, Hangzhou 310014, China. feibaoying6924@tom.com
Bao-Ying
BY
Fei
Huo-Xiang
HX
Lv
Jiang-Min
JM
Yang
Zai-Yuan
ZY
Ye
D000328:Adult; D044466:Asian People; D002681:China; D003093:Colitis, Ulcerative / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000235:genetics*; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
D008263:Macrophage Migration-Inhibitory Factors; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human