Stem cells and combinatorial science.
Stem cell-based technologies have the potential to help cure a number of cell degenerative diseases. Combinatorial and high throughput screening techniques could provide tools to control and manipulate the self-renewal and differentiation of stem cells. This review chronicles historic and recent progress in the stem cell field involving both pluripotent and multipotent cells, and it highlights relevant cellular signal transduction pathways. This review further describes screens using libraries of soluble, small-molecule ligands, and arrays of molecules immobilized onto surfaces while proposing future trends in similar studies. It is hoped that by reviewing both the stem cell and the relevant high throughput screening literature, this paper can act as a resource to the combinatorial science community.
Fang
Yue Qin
YQ
Division of Chemistry and Biological Chemistry, Nanyang Technological University, 1 Nanyang Walk, Block 5, Level 3, Singapore.
Wong
Wan Qing
WQ
Yap
Yan Wen
YW
Orner
Brendan P
BP
D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology; D008024:Ligands; D046228:Microarray Analysis / Q000379:methods; D010641:Phenotype; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology; D012038:Regeneration / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*
D008024:Ligands