Amine-guanidine switch: a promising approach to improve DNA binding and antiproliferative activities.
A series of polyaromatic guanidino derivatives was synthesized and evaluated for growth inhibitory properties in several human carcinoma cell lines. The properties of these guanidino compounds were compared to those of their corresponding synthetic amino precursors. The size of the polyaromatic ring system as well as the length of the tether attached to the ring had a direct impact on the observed antiproliferative profiles, compound 14 having the broadest spectrum of activity. As both series intercalate DNA, guanidine derivatives showed a remarkable affinity for DNA and the guanidinium group appeared to be essential, yet not sufficient for caspase-3/7 activation. Compound 14 also showed significant in vivo activity against breast cancer cell xenografts in NOG/SCID mice. These results suggest that the electronic nature of chain tethering an intercalator not only influences the DNA-binding process but also controls the antitumoral activity of the whole compound.
Ohara
Keiichiro
K
Institut des Biomolécules Max Mousseron, UMR 5247 CNRS-UM I-UM II, Université de Montpellier II, France.
Smietana
Michael
M
Restouin
Audrey
A
Mollard
Séverine
S
Borg
Jean-Paul
JP
Collette
Yves
Y
Vasseur
Jean-Jacques
JJ
D000588:Amines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D045744:Cell Line, Tumor; D004247:DNA / Q000378:metabolism*; D004354:Drug Screening Assays, Antitumor; D004789:Enzyme Activation; D006146:Guanidines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007364:Intercalating Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D013329:Structure-Activity Relationship; D044366:Transition Temperature; D014183:Transplantation, Heterologous
D000588:Amines; D000970:Antineoplastic Agents; D006146:Guanidines; D007364:Intercalating Agents; D004247:DNA; D053148:Caspase 3; D053179:Caspase 7