A highly selective fluorescent probe for thiol bioimaging.
A new fluorescent turn-on probe (3) for the selective sensing and bioimaging of thiols is reported. In aqueous buffer solutions at physiological pH, thiols cleave the 2,4-dinitrobenzenesulfonyl group to release the red-emissive donor-acceptor fluorophore (4). The probe displays excellent immunity to interference from nitrogen and oxygen nucleophiles and the imaging of thiols in living cells is demonstrated.
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Jean
J
Bouffard
Youngmi
Y
Kim
Timothy M
TM
Swager
Ralph
R
Weissleder
Scott A
SA
Hilderbrand
D003124:Colorimetry / Q000379:methods; D003952:Diagnostic Imaging / Q000379:methods*; D005456:Fluorescent Dyes; D015394:Molecular Structure; D009578:Nitrobenzenes / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000032:analysis*
D005456:Fluorescent Dyes; D009578:Nitrobenzenes; D013438:Sulfhydryl Compounds; D009584:Nitrogen; D010100:Oxygen