Transgene expression facilitated by the v-src splice acceptor can impair replication kinetics and lead to genomic instability of Rous sarcoma virus-based vectors.
Rous sarcoma virus (RSV) can be used for the simple generation of high-titer replication-competent retroviral (RCR) vectors. Retroviruses undergo frequent genomic recombination, however, and vectors with reduced replication kinetics are rapidly overgrown by mutant forms. Vector design is hence critical to vector efficacy. In this study, two different designs of RSV-based RCR vectors were evaluated. Vectors in which transgene expression was facilitated by the v-src splice acceptor were revealed to have greatly reduced replication kinetics and genomic stability in comparison to vectors in which transgene expression was mediated by an internal ribosome entry site in the 3' untranslated region.
Portsmouth
Daniel
D
Research Institute of Virology and Biomedicine, University of Veterinary Medicine, Vienna, Austria.
Deitermann
Daria
D
Salmons
Brian
B
Günzburg
Walter H
WH
Renner
Matthias
M
D005822:Genetic Vectors; D042822:Genomic Instability; D022821:RNA Splice Sites; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D052736:Rous sarcoma virus / Q000235:genetics*; D019076:Transgenes
D022821:RNA Splice Sites; D011994:Recombinant Proteins