[Cloning and construction of nucleic acid vaccine of FABP gene cDNA from Echinococcus granulosus].
Specific primers were designed according to published nucleotide sequence of FABP (fatty acid binding protein) gene in the GenBank database. The kozak sequence (CCACC) was introduced at the upstream of initiator. The total RNA was extracted from protoscoleces of Echinococcus granulosus (Inner Mongol isolate). The FABP gene cDNA fragment was amplified by RT-PCR and cloned into pMD19-T vector for sequencing and analyzing. The cloned FABP gene cDNA was with 402bp. The ORF encoded 133 amino acids. The amplified cDNA fragment was subcloned into pCDNA3.1 (+) vector. The results showed that the nucleic acid vaccine candidate pcDNA-FABP-NM has been constructed.
Hao
Hui-fang
HF
College of Life Science, Inner Mongolia University, Huhehaot 010021, China.
Wang
Zhi-gang
ZG
Li
Zhi-wei
ZW
D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D048209:Echinococcus granulosus / Q000235:genetics* / Q000276:immunology; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000276:immunology; D015801:Helminth Proteins / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA; D019444:Vaccines, DNA / Q000235:genetics* / Q000276:immunology
D018076:DNA, Complementary; D050556:Fatty Acid-Binding Proteins; D015801:Helminth Proteins; D019444:Vaccines, DNA