A comparative study of mechanisms of surfactant inhibition.
Pulmonary surfactant spreads to the hydrated air-lung interface and reduces the surface tension to a very small value. This function fails in acute respiratory distress syndrome (ARDS) and the surface tension stays high. Dysfunction has been attributed to competition for the air-lung interface between plasma proteins and surfactant or, alternatively, to ARDS-specific alterations of the molecular profile of surfactant. Here, we compared the two mechanisms in vitro, to assess their potential role in causing respiratory distress. Albumin and fibrinogen exposure at or above blood level concentrations served as the models for testing competitive adsorption. An elevated level of cholesterol was chosen as a known adverse change in the molecular profile of surfactant in ARDS. Bovine lipid extract surfactant (BLES) was spread from a small bolus of a concentrated suspension (27 mg/ml) to the air-water interface in a captive bubble surfactometer (CBS) and the bubble volume was cyclically reduced and increased to assess surface activity of the spread material. Concentrations of inhibitors and the concentration and spreading method of pulmonary surfactant were chosen in an attempt to reproduce the exposure of surfactant to inhibitors in the lung. Under these conditions, neither serum albumin nor fibrinogen was persistently inhibitory and normal near-zero minimum surface tension values were obtained after a small number of cycles. In contrast, inhibition by an increased level of cholesterol persisted even after extensive cycling. These results suggest that in ARDS, competitive adsorption may not sufficiently explain high surface tension, and that disruption of the surfactant film needs to be given causal consideration.
Department of Cell Biology and Anatomy, The University of Calgary, Health Science Centre 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1.
Lasantha
L
Gunasekara
W Michael
WM
Schoel
Samuel
S
Schürch
Matthias W
MW
Amrein
D001798:Blood Proteins / Q000502:physiology; D020285:Cryoelectron Microscopy; D011663:Pulmonary Surfactants / Q000037:antagonists & inhibitors*
D001798:Blood Proteins; D011663:Pulmonary Surfactants