Evaluation for protein binding affinity of maghemite and magnetite nanoparticles.
We investigated the protein binding affinity of magnetite (Fe3O4) and maghemite (gamma-Fe2O3) nanoparticles with against non-characterized protein from human lung cancer A549 cell line on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The binding ability of maghemite was 400 ng/mg. According to the SDS-PAGE results, the protein binding affinity of maghemite nanoparticles is stronger than magnetite nanoparticles. These data suggest that a protein can be detected with maghemite nanoparticles.
Gyeonggi Pharmaceutical Research Center, Gyeonggi Bio-Center, Suwon 443-766, Korea.
Se Chan
SC
Kang
Yong Jun
YJ
Jo
Jong Phil
JP
Bak
Ki-Chul
KC
Kim
Young-Sung
YS
Kim
D002460:Cell Line; D016503:Drug Delivery Systems / Q000379:methods; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005296:Ferrous Compounds / Q000737:chemistry*; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry*; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000737:chemistry*; D011485:Protein Binding
D005296:Ferrous Compounds; D009363:Neoplasm Proteins; C034236:ferrous oxide; D052203:Ferrosoferric Oxide