Zinc metal solubilization by Gluconacetobacter diazotrophicus and induction of pleomorphic cells.
Gluconacetobacter diazotrophicus strain PAl5 exhibited a minimum inhibitory concentration value of 11 mM in an LGI medium amended with ZnCl2. When an LGI medium was amended with Zn metal, solubilization halos were observed in a plate assay, and further solubilization was confirmed in a broth assay. The maximum solubilization was recorded after 120 h with a 0.1% Zn metal amendment. During solubilization, the culture growth and pH of the broth were indirectly correlated. Using a Fourier Transform Infrared Spectroscopy analysis, one of the agents solubilizing the Zn metal was identified as gluconic acid. When the Zn-amended broth was observed under a bright field microscope, long involution cells were observed, and further analysis with Atomic Force Microscopy revealed highly deformed, pleomorphic, aggregate-like cells.
Department of Agricultural Chemistry, Chungbuk National University, Cheongju 361-763, Korea.
Venkatakrishnan Sivaraj
VS
Saravanan
Jabez
J
Osborne
Munusamy
M
Madhaiyan
Lazar
L
Mathew
Jongbae
J
Chung
Kisup
K
Ahn
Tongmin
T
Sa
D003470:Culture Media / Q000737:chemistry; D041821:Gluconacetobacter / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D012995:Solubility; D015032:Zinc / Q000737:chemistry*; D017967:Zinc Compounds / Q000378:metabolism*
D003470:Culture Media; D017967:Zinc Compounds; D015032:Zinc