Peptidoglycan recognition protein-S (PGRP-S) is upregulated by NF-kappaB.
Cerebral ischemia triggers inflammation and apoptosis, and the transcription factor NF-kappaB is a key regulator of both events. Here, we report on the induction of the peptidoglycan recognition protein-S (PGRP-S) in a mouse model of cerebral ischemia. Upregulation was reduced if the NF-kappaB subunit RelA was conditionally deleted in the brain. Regulation of PGRP-S transcription by RelA was confirmed in vitro. Cotransfection of a RelA expression plasmid stimulated the expression of a PGRP-S luciferase fusion gene. Mutation of two NF-kappaB response elements in the PGRP-S promoter disrupted stimulation by RelA. To investigate the function of PGRP-S in cerebral ischemia, we subjected PGRP-S(-/-) mice to cerebral ischemia. However, there was no difference in the infarct size in PGRP-S-deficient mice compared to controls. In summary, the data show that PGRP-S is induced in cerebral ischemia by RelA, but its role in ischemia is unclear.
Lang
Ming-Fei
MF
Institute of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, 69120 Heidelberg, Germany. ming-fei.lang@pharma.uni-heidelberg.de
Schneider
Armin
A
Krüger
Carola
C
Schmid
Roland
R
Dziarski
Roman
R
Schwaninger
Markus
M
D000704:Analysis of Variance; D000818:Animals; D002545:Brain Ischemia / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D009154:Mutation; D016716:PC12 Cells; D051381:Rats; D051996:Transcription Factor RelA / Q000502:physiology*; D014162:Transfection; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology*
D002352:Carrier Proteins; C498144:Rela protein, mouse; D051996:Transcription Factor RelA; C118897:peptidoglycan recognition protein