CT60 A/G marker of the 3'-UTR of the CTLA4 gene and liver transplant.
The CTLA4 molecule is a homolog for CD28, and both molecules and their common ligands (CD80 and CD86) constitute the B7/CD28-CTLA4 co-stimulatory pathway for T cells activation. The CTLA4-ligand interaction has an inhibitory effect on T cell activation and might contribute to peripheral tolerance. A recently described CT60 A/G polymorphism affects the production of soluble CTLA4 and is strongly associated with immune-mediated diseases and with allogenic stem cell transplantation outcome. Thus, we examined this marker on liver transplant outcome by a PCR-RFLP method. The CT60 G allele was significantly associated with acute rejection (Pc=0.038; OR=1.49; AR vs. NAR). Patients who lacked this allele had the lowest risk of acute rejection development. Allograft survival data did not show statistical differences between genotypes. In conclusion, CT60 A/G dimorphism within the 3'-UTR of CTLA4 gene, which encodes for reduced sCTLA4 production, influence acute rejection development in liver transplantation.
Immunology Service, University Hospital Virgen de la Arrixaca, Murcia 30120, Spain. manuel.muro@carm.es
Manuel
M
Muro
Gloria
G
Rojas
Carmen
C
Botella
Manuel
M
Miras
José A
JA
Campillo
Alfredo
A
Minguela
Francisco
F
Sánchez-Bueno
Juan
J
Bermejo
Pablo
P
Ramírez
María R
MR
Alvarez-López
D020413:3' Untranslated Regions; D000328:Adult; D015703:Antigens, CD / Q000235:genetics*; D000943:Antigens, Differentiation / Q000235:genetics*; D060908:CTLA-4 Antigen; D005260:Female; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology; D006085:Graft Survival; D006801:Humans; D007166:Immunosuppressive Agents / Q000378:metabolism; D053208:Kaplan-Meier Estimate; D016031:Liver Transplantation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
D020413:3' Untranslated Regions; D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D007166:Immunosuppressive Agents