Black rice (Oryza sativa L. var. japonica) hydrolyzed peptides induce expression of hyaluronan synthase 2 gene in HaCaT keratinocytes.
Black rice (Oryza sativa L. var. japonica) has been used in folk medicine in Asia. To understand the effects of black rice hydrolyzed peptides (BRP) from germinated black rice, we assessed the expression levels of about 20,000 transcripts in BRP-treated HaCaT keratinocytes using human 1A oligo microarray analysis. As a result, the BRP treatment showed a differential expression ratio of more than 2-fold: 745 were activated and 1,011 were repressed. One of the most interesting findings was a 2-fold increase in hyaluronan synthase 2 (HAS2) gene expression by BRP. Semiquantitative RT-PCR showed that BRP increased HAS2 mRNA in dose-dependent manners. ELISA showed that BRP effectively increased hyaluronan (HA) production in HaCaT keratinocytes.
R&D Center Hanbul Cosmetics Co. Ltd., Chungbuk 369-834, Korea.
Gwan Sub
GS
Sim
Dong-Hwan
DH
Lee
Jin-Hwa
JH
Kim
Sung-Kwan
SK
An
Tae-Boo
TB
Choe
Tae-Jong
TJ
Kwon
Hyeong-Bae
HB
Pyo
Bum-Chun
BC
Lee
D002478:Cells, Cultured; D020869:Gene Expression Profiling; D014453:Glucuronosyltransferase / Q000235:genetics*; D006801:Humans; D000076002:Hyaluronan Synthases; D006868:Hydrolysis; D015603:Keratinocytes / Q000201:enzymology*; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000737:chemistry*; D010940:Plant Proteins / Q000494:pharmacology*
D010940:Plant Proteins; D014453:Glucuronosyltransferase; C101754:HAS2 protein, human; D000076002:Hyaluronan Synthases