A highly conserved protein of unknown function is required by Sinorhizobium meliloti for symbiosis and environmental stress protection.
We report here the first characterization of the Sinorhizobium meliloti open reading frame SMc01113. The SMc01113 protein is a member of a highly conserved protein family, universal among bacteria. We demonstrate that the SMc01113 gene is absolutely required for S. meliloti symbiosis with alfalfa and also for the protection of the bacterium from a wide range of environmental stresses.
Davies
Bryan W
BW
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Walker
Graham C
GC
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017124:Conserved Sequence; D004777:Environment; D015964:Gene Expression Regulation, Bacterial; D018869:Heat-Shock Response; D000455:Medicago sativa / Q000254:growth & development / Q000382:microbiology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D013559:Symbiosis
D001426:Bacterial Proteins