Effect of siRNA targeted against MKK4 on myostatin-induced downregulation of differentiation marker gene expression.
The c-Jun N-terminal kinase (JNK) pathway was reported to be involved in myostatin signaling and MKK4 was suggested as the only upstream kinase for myostatin-induced JNK activation, implying that MKK4 is a suitable target of RNA interference (RNAi) for blocking myostatin activity. The aim of this study was to evaluate the effect of small interfering RNA (siRNA) targeted against MKK4 on myostatin-induced downregulation of differentiation marker gene expression. Real-time quantitative PCR revealed that the level of MKK4 expression was efficiently reduced by MKK4-specific siRNA. Western blot assays showed that knockdown of MKK4 attenuated the myostatin-induced downregulation of MyoD and myogenin expression.
Huang
Zhiqing
Z
Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan 625014, PR China.
Zhang
Keying
K
Chen
Xiaoling
X
Meng
Jianghong
J
Chen
Daiwen
D
D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics*; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects*; D048670:MAP Kinase Kinase 4 / Q000037:antagonists & inhibitors*; D051379:Mice; D055435:Myostatin; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D000943:Antigens, Differentiation; C525912:Mstn protein, mouse; D055435:Myostatin; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D016212:Transforming Growth Factor beta; D048670:MAP Kinase Kinase 4; C482562:Map2k4 protein, mouse