Identification of epithelial gaps in human small and large intestine by confocal endomicroscopy.
OBJECTIVE
Confocal endomicroscopy is an emerging technology that poses the endoscopist with challenges for identifying epithelial structures in the human intestine. We have shown previously that the murine intestinal epithelium is punctuated by gaps caused by cell shedding. The goals of this study were to determine if confocal endomicroscopy could resolve the presence of human epithelial gaps and whether a proinflammatory cytokine could increase cell shedding.


METHODS
Intestinal mucosa was imaged after staining with acriflavine. Confocal endomicroscopy of 17 patients yielded 6277 images from the human terminal ileum and rectum. Results were validated by parallel studies of anesthetized mice (wild-type and Math1(DeltaIntestine)) using rigid confocal probe microscopy, 2-photon/confocal microscopy, and scanning electron microscopy.


RESULTS
Human terminal ileal and rectal epithelium revealed unstained areas with the diameter of an individual epithelial cell, with 2 distinct morphologies. One had a "target" appearance, shown by mouse studies to be goblet cells. The other morphology had no nucleus and was observed by rigid confocal probe microscopy and scanning electron microscopy in the villi of Math1(DeltaIntestine) mice, which lack goblet cells. In the mouse, tumor necrosis factor alpha (0.33 microg/g intraperitoneally) increases cell shedding by 27-fold and caused loss of barrier function across 20% of resultant gaps.


CONCLUSIONS
Confocal endomicroscopy can distinguish between epithelial discontinuities (gaps) and goblet cells in human intestine. Results suggest that the sealing of epithelial gaps must be considered as a component of the intestinal barrier and has potential implications for intestinal barrier dysfunction in human disease.
Kiesslich
Ralf
R
I Medical Clinic, University of Mainz, Mainz, Germany.
Goetz
Martin
M
Angus
Elizabeth M
EM
Hu
Qiuping
Q
Guan
Yanfang
Y
Potten
Chris
C
Allen
Terry
T
Neurath
Markus F
MF
Shroyer
Noah F
NF
Montrose
Marshall H
MH
Watson
Alastair J M
AJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D003113:Colonoscopy; D004847:Epithelial Cells / Q000473:pathology*; D005260:Female; D006801:Humans; D007420:Intestine, Large / Q000473:pathology*; D007421:Intestine, Small / Q000473:pathology*; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal; D008875:Middle Aged; D014409:Tumor Necrosis Factor-alpha
D014409:Tumor Necrosis Factor-alpha