[Contribution of the genetic fingerprintings compared to grouping ABO/Rhesus technique in the expertise of filiation].
Paternity is based on biological analyzes that have drastically developed during the past 20 years. According to scientific developments, paternity testing was based on red blood groups studies, the analysis of red cell enzymes and plasma proteins polymorphisms, the typing of the HLA antigens, and the DNA polymorphism in its various forms. This study aims at comparing two analyses: red blood groups and DNA polymorphism. The performance of each test is analyzed in this report, based on a study of 142 cases. Indeed, the numbers of case of paternity exclusion are respectively 6 and 45 by the classic method and the genetic one. Thanks to studies based on the gene amplification of microsatellites, the efficiency of this reference technique has been proved, however, the classic one makes it possible in the cases of exclusion to lead to a certain decision without recourse to other systems. Of these facts, beyond the most efficient biological analysis, it is very important to think about paternity testing as a process in which biological tests are only one step.
Laboratoire de biologie moléculaire, Hôpital Ibn El Jazzar, Kairouan, Tunisia. souidenyousra@yahoo.fr
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Y
Souiden
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Chaieb
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M
Romdhani
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K
Mahdouani
D000017:ABO Blood-Group System / Q000235:genetics* / Q000302:isolation & purification; D001789:Blood Group Antigens / Q000235:genetics* / Q000302:isolation & purification; D002648:Child; D016172:DNA Fingerprinting / Q000379:methods*; D019075:Electrophoresis, Capillary; D005260:Female; D006680:HLA Antigens / Q000235:genetics*; D006801:Humans; D008297:Male; D009679:Nuclear Family; D010334:Paternity; D011110:Polymorphism, Genetic; D012204:Rh-Hr Blood-Group System / Q000235:genetics* / Q000302:isolation & purification
D000017:ABO Blood-Group System; D001789:Blood Group Antigens; D006680:HLA Antigens; D012204:Rh-Hr Blood-Group System