Electrophysiological recordings from the neuromuscular junction of C. elegans.
Electrophysiology provides a quantifiable measure of synaptic activity useful in the functional analysis of synaptic proteins. Recent advances in the application of this technique to C. elegans provides a means of coupling genetics to electrophysiological analysis, providing new insights into the molecular mechanisms regulating neurotransmission. Here we describe a dissection technique that exposes the neuromuscular junctions of C. elegans for electrophysiological analysis. This technique can be adapted to record from virtually any excitable cell in the worm.
Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA. jer@uic.edu
Janet E
JE
Richmond
D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D004594:Electrophysiology / Q000379:methods*; D009469:Neuromuscular Junction / Q000502:physiology*