Characterization of a fucoidan from Lessonia vadosa (Phaeophyta) and its anticoagulant and elicitor properties.
Blades of Lessonia vadosa (Phaeophyta) were extracted with 2% CaCl(2) solution, affording in 4.4% yield a polysaccharide which contained fucose and sulfate groups in the molar ratio 1.0:1.12. The high negative optical activity value ([alpha](D)(22)=-134.0 degrees ), FT-IR and NMR analysis suggest the presence of a fucoidan. (13)C NMR spectrum of the polysaccharide obtained by solvolytic desulfation of native fucoidan indicated the major presence of 1-->3 linked alpha-l-fucan. Depolymerization of the native fucoidan with H(2)O(2) in the presence of copper(II) acetate gave in 54.8% yield a fraction with 33.7% of sulfate content. The native fucoidan (MW 320,000) showed good anticoagulant activity whereas the radical depolymerized fraction (MW 32,000) presented a weak anticoagulant activity. These polysaccharides showed significant activation of phenylalanine-ammonia lyase (PAL), lipooxygenase (LOX) and glutathione-S-transferase (GST) defence enzyme activities in tobacco plants.
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Departamento de Ciencias del Ambiente, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago, Chile.
Matsuhiro
B
B
D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification; D002236:Carbohydrate Conformation; D004789:Enzyme Activation; D005982:Glutathione Transferase / Q000737:chemistry; D008084:Lipoxygenase / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D000459:Phaeophyceae / Q000737:chemistry*; D010650:Phenylalanine Ammonia-Lyase / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D017550:Spectroscopy, Fourier Transform Infrared; D014026:Nicotiana / Q000201:enzymology
D000925:Anticoagulants; D010940:Plant Proteins; D011134:Polysaccharides; C007789:fucoidan; D008084:Lipoxygenase; D005982:Glutathione Transferase; D010650:Phenylalanine Ammonia-Lyase