A mini-library of TBA analogues containing 3'-3' and 5'-5' inversion of polarity sites.
Several researches have been devoted to structure-activity relationship and to post-SELEX modifications of the thrombin binding aptamer (TBA), one of the first aptamers discovered by the SELEX methodology. However, no studies on TBA dealing with the effects of introduction of inversion of polarity sites have been reported yet. In this frame, we have undertaken the synthesis and the study of a mini-library composed of several TBA analogues containing a 3'-3' or a 5'-5' inversion of polarity site at different positions into the sequence. Particularly, in this article, we present preliminary results about their structural and biological properties.
Esposito
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Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli Federico II, Napoli, Italy.
Galeone
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Mayol
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Randazzo
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Virgilio
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Virno
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D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D002942:Circular Dichroism; D015723:Gene Library; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D052156:SELEX Aptamer Technique; D013816:Thermodynamics; D013917:Thrombin / Q000037:antagonists & inhibitors*
D052157:Aptamers, Nucleotide; C081676:thrombin aptamer; D013917:Thrombin