Biocompatibility and gelation of chitosan-glycerol phosphate hydrogels.
The aim of this study was to evaluate the cytotoxicity and gelation of thermosensitive chitosan-beta-glycerol phosphate (GP) solutions, which undergo sol-gel transition around body temperature. Chitosan 0.5-2% (w/v) mixed with GP 5-20% (w/v) solutions all gel at 37 degrees C and possess pH around the physiological range. High GP and chitosan concentrations result in faster gelation time. Extracts of all chitosan concentrations mixed with or without 5% (w/v) GP and 2% (w/v) chitosan combined with 10% (w/v) GP demonstrated up to 34% increase in proliferation rate of goat bone marrow derived mesenchymal stem cells when compared with control medium. Extracts from all other chitosan-GP combinations resulted in reduced cell proliferation relative to control medium. Increasing GP content in the gel resulted in a linear increase in the osmolality of the extracts in contact with the gels. The results of this study indicate that chitosan-GP is a biocompatible hydrogel, extracts of which can stimulate mesenchymal stem cell proliferation at certain concentrations. This material is therefore a promising vehicle for cell encapsulation and injectable tissue-engineering applications.
School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, United Kingdom.
Raheleh
R
Ahmadi
Joost D
JD
de Bruijn
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000494:pharmacology*; D003470:Culture Media; D005994:Glycerophosphates / Q000494:pharmacology*; D006041:Goats; D020100:Hydrogels / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D009994:Osmolar Concentration; D012996:Solutions; D013997:Time Factors
D001672:Biocompatible Materials; D003470:Culture Media; D005994:Glycerophosphates; D020100:Hydrogels; D012996:Solutions; D048271:Chitosan; C031463:beta-glycerophosphoric acid