Structural basis for the coevolution of a viral RNA-protein complex.
The cocrystal structure of the PP7 bacteriophage coat protein in complex with its translational operator identifies a distinct mode of sequence-specific RNA recognition when compared to the well-characterized MS2 coat protein-RNA complex. The structure reveals the molecular basis of the PP7 coat protein's ability to selectively bind its cognate RNA, and it demonstrates that the conserved beta-sheet surface is a flexible architecture that can evolve to recognize diverse RNA hairpins.
Department of Anatomy, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, New York 10461, USA.
Jeffrey A
JA
Chao
Yury
Y
Patskovsky
Steven C
SC
Almo
Robert H
RH
Singer
D001435:Bacteriophages / Q000737:chemistry / Q000235:genetics; D001665:Binding Sites; D018360:Crystallography, X-Ray; D019143:Evolution, Molecular; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D012328:RNA Viruses / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D012367:RNA, Viral; D014764:Viral Proteins