A rare case of lung coinfection by Streptomyces cinereoruber and Haemophilus influenzae in a patient with severe chronic obstructive pulmonary disease: characterization at species level using molecular techniques.
Streptomyces cinereoruber isolated from a patient with severe respiratory failure that could not be identified by culture was correctly identified at the species level by comparative sequence analysis of 16S rDNA and the hypervariable alpha-region of 16S rDNA. Restriction fragment length polymorphism, morphologic, and biochemical characteristics have confirmed their identity.
Area de Microbiologia, Departamento de Biologia Funcional, Instituto Universitario de Biotecnologia de Asturias (IUBA), Universidad de Oviedo, 33006 Oviedo, Asturias, Spain. mantecaangel@uniovi.es
Angel
A
Manteca
Ana Isabel
AI
Pelaez
Maria
M
del Mar Garcia-Suarez
Elisa
E
Hidalgo
Benigno
B
del Busto
Francisco J
FJ
Mendez
D000368:Aged; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006192:Haemophilus Infections / Q000150:complications* / Q000188:drug therapy; D006193:Haemophilus influenzae / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D011241:Prednisone / Q000627:therapeutic use; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013902:Radiography, Thoracic; D013302:Streptomyces / Q000235:genetics / Q000302:isolation & purification*; D013420:Sulfamethoxazole / Q000627:therapeutic use; D014057:Tomography, X-Ray Computed; D014295:Trimethoprim / Q000627:therapeutic use
D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D014295:Trimethoprim; D013420:Sulfamethoxazole; D011241:Prednisone