Identification and characterization of cyclin X which activates transcriptional activities of c-Myc.
Cyclins are eukaryotic proteins that play an active role in controlling cell division cycles and regulate cyclin dependent kinases (CDKs). In this study, we identified and characterized a new member of the human cyclin family. Its cDNA sequence contains an open reading frame of 864 bp, encoding a protein of 287 amino acids. This putative cyclin, designated cyclin X, contains a typical cyclin box. Cyclin X shows the highest sequence similarity in cyclin box to cyclin B3 and the similarity between these two proteins also extends to the whole molecule. The expression pattern of cyclin X showed that it was highly expressed in testis. Cyclin X protein was located in nucleus of the H1299 cells and Overexpression of cyclin X activated the transcriptional activities of c-Myc. These results indicate that cyclin X is a novel member of the human cyclin family and positive regulator of c-Myc-mediated gene transcription.
Institute of Genetics, School of Life Science, Fudan University, Shanghai, 200433, People's Republic of China.
Xuanyang
X
Li
Xiang
X
Wang
Guoyuan
G
Liu
Rui
R
Li
Long
L
Yu
D000595:Amino Acid Sequence; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D019926:Cyclin B / Q000235:genetics; D016213:Cyclins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation; D016259:Genes, myc; D006801:Humans; D008969:Molecular Sequence Data; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D016415:Sequence Alignment; D046888:Tissue Array Analysis; D015533:Transcriptional Activation
D019926:Cyclin B; D016213:Cyclins; D016271:Proto-Oncogene Proteins c-myc; C503694:CCNB3 protein, human; C489427:MYC protein, human; C543319:CCNY protein, human