Effect of panaxatriol on hematogenesis and granulocyte-macrophage colony stimulating factor in radiation injured mice.
OBJECTIVE
To assess the recovery effect of panaxatriol (PT) on myeloid hemopoiesis in radiation injured mice, and analyze the underlying mechanism.


METHODS
This study was carried out in the Animal Center of Shandong University, Jinan, China, during March to September 2006. Forty-five inbred albino mice were separated randomly into 3 groups: control group, radiation group, and radiation + PT group (200 mg/kg/d, 3 weeks). Peripheral blood cells were detected by globuli meter, CD34+ cells in bone marrow were detected by flow cytometry, and the protein expression of granulocyte-macrophage colony stimulating factor (GM-CSF) was detected by immunocytochemistry.


RESULTS
The numbers of peripheral blood cells and bone marrow CD34+ cells, and the expression of GM-CSF in the radiation group were lower than in the control group. After treatment with PT, the numbers of peripheral blood cells and CD34+ cells, and the expression of GM-CSF increased significantly.


CONCLUSIONS
Panaxatriol can relieve myelosuppression induced by radiation injury. The abilities of regulating the expression of hemopoietic growth factor GM-CSF and promoting the maturation of bone marrow cells may be responsible for some of these beneficial effects.
Liu
Fen Y
FY
Department of Traditional Chinese Medicine, Qilu Hospital of Shandong University, Jinan, Shandong, China. yuliufenye@126.com
Wang
Jun N
JN
Yu
Shu D
SD
Wang
Bo
B
Zhang
Ji D
JD
D000818:Animals; D001772:Blood Cell Count; D001854:Bone Marrow Cells / Q000378:metabolism; D036145:Ginsenosides / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D006410:Hematopoiesis / Q000187:drug effects*; D006454:Hemoglobins / Q000378:metabolism; D051379:Mice; D011832:Radiation Injuries / Q000097:blood*; D014315:Triterpenes / Q000494:pharmacology*
D036145:Ginsenosides; D006454:Hemoglobins; D014315:Triterpenes; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; C004563:panaxatriol