Characterization of monomeric and oligomeric flavan-3-ols from barley and malt by liquid chromatography-ultraviolet detection-electrospray ionization mass spectrometry.
The pattern of the monomeric and oligomeric flavan-3-ols for 10 barley varieties and the corresponding malts were identified and quantified using high-performance liquid chromatography-ultraviolet detection-electrospray ion trap mass spectrometry. The Folin-Ciocalteau and the vanillin spectrophotometric assays were used for the assessment of the total polyphenol and total flavan-3-ol content, respectively, and the antioxidant activity was determined as the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and the ferricyanide reducing power. Catechin and prodelphinidin B3 were respectively the major monomeric and dimeric flavan-3-ols. Moreover, prodelphinidin B3 was shown to be the main contributor for the radical scavenging activity both for barley and malt.
Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology, Technicka 5, 166 28 Prague, Czech Republic. marketa.dvorakova@vscht.cz
Marketa
M
Dvorakova
Manuela M
MM
Moreira
Pavel
P
Dostalek
Zuzana
Z
Skulilova
Luís F
LF
Guido
Aquiles A
AA
Barros
D000872:Anthocyanins / Q000032:analysis; D000975:Antioxidants / Q000032:analysis; D002392:Catechin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D001467:Hordeum / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000872:Anthocyanins; D000975:Antioxidants; D005419:Flavonoids; C472180:prodelphinidin B-2 3'-O-gallate; C404987:flavan-3-ol; D002392:Catechin