Physical mapping in large genomes: accelerating anchoring of BAC contigs to genetic maps through in silico analysis.
Anchored physical maps represent essential frameworks for map-based cloning, comparative genomics studies, and genome sequencing projects. High throughput anchoring can be achieved by polymerase chain reaction (PCR) screening of bacterial artificial chromosome (BAC) library pools with molecular markers. However, for large genomes such as wheat, the development of high dimension pools and the number of reactions that need to be performed can be extremely large making the screening laborious and costly. To improve the cost efficiency of anchoring in such large genomes, we have developed a new software named Elephant (electronic physical map anchoring tool) that combines BAC contig information generated by FingerPrinted Contig with results of BAC library pools screening to identify BAC addresses with a minimal amount of PCR reactions. Elephant was evaluated during the construction of a physical map of chromosome 3B of hexaploid wheat. Results show that a one dimensional pool screening can be sufficient to anchor a BAC contig while reducing the number of PCR by 384-fold thereby demonstrating that Elephant is an efficient and cost-effective tool to support physical mapping in large genomes.
Paux
Etienne
E
INRA, UMR 1095, ASP, 234, avenue du Brézet, 63100, Clermont-Ferrand, France.
Legeai
Fabrice
F
Guilhot
Nicolas
N
Adam-Blondon
Anne-Françoise
AF
Alaux
Michaël
M
Salse
Jérôme
J
Sourdille
Pierre
P
Leroy
Philippe
P
Feuillet
Catherine
C
D002875:Chromosomes / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D019295:Computational Biology / Q000379:methods*; D020451:Contig Mapping / Q000379:methods*; D016678:Genome / Q000235:genetics*; D018598:Minisatellite Repeats / Q000235:genetics; D015203:Reproducibility of Results; D012984:Software