Inhibitory effect of cyclic terpenes (limonene, menthol, menthone and thymol) on Fusarium verticillioides MRC 826 growth and fumonisin B1 biosynthesis.
The minimum inhibitory concentration (MIC) of cyclic terpenes (limonene, menthol, menthone and thymol) against Fusarium verticillioides MRC 826 was assessed by using the semisolid agar antifungal susceptibility (SAAS) technique. Limonene, menthol, menthone and thymol were evaluated at final concentrations of 25, 50, 75, 150, 200, 250, 500 and 1000 microL/L of culture medium. Limonene and thymol showed the highest inhibitory effects on F. verticillioides development. Thus, the effects of monoterpenes on fumonisin B1 (FB1) biosynthesis were evaluated by using corn grain (Zea mays) as substrate. The monoterpenes were inserted on maize 1 day before inoculation with a conidial suspension of F. verticillioides to give final concentrations of 75 ppm. At this concentration, thymol was the most active inhibitor on FB1 biosynthesis.
IMBIV (CONICET) Cátedra de Química Orgánica, Facultad de Ciencias Exactas Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, Argentina.
J S
JS
Dambolena
A G
AG
López
M C
MC
Cánepa
M G
MG
Theumer
J A
JA
Zygadlo
H R
HR
Rubinstein
D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D053138:Cyclohexenes / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D037341:Fumonisins / Q000378:metabolism*; D005670:Fusarium / Q000187:drug effects* / Q000254:growth & development*; D000077222:Limonene; D008610:Menthol / Q000737:chemistry / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D013729:Terpenes / Q000737:chemistry / Q000494:pharmacology; D013943:Thymol / Q000737:chemistry / Q000494:pharmacology
D000935:Antifungal Agents; D053138:Cyclohexenes; D037341:Fumonisins; D013729:Terpenes; D008610:Menthol; D013943:Thymol; C056933:fumonisin B1; D000077222:Limonene; C019466:menthone