Persistent antibody and T cell responses induced by HIV-1 DNA vaccine delivered by electroporation.
Intramuscular needle injection of HIV-1 DNA vaccines typically elicits weak immune responses in immunized individuals. To improve such responses, the immunogenicity of a vaccine consisting of electroporated DNA followed by intramuscular protein boost was evaluated in rabbits and macaques. In macaques, electroporation of low dose DNA encoding HIV-1 env followed by gp120 protein elicited Th1 cytokines and functional CTL that persisted for over 1 year. In both macaques and rabbits, robust anti-envelope antibodies, elicited by electroporated DNA, were augmented by gp120 protein and such responses neutralized sensitive SHIV isolates. These findings highlight efficient priming of immune responses by electroporated DNA that in conjunction with protein boost may give rise to long-term immunity in immunized hosts.
Cristillo
Anthony D
AD
Advanced BioScience Laboratories, Inc., Department of Cell Biology, 5510 Nicholson Lane, Kensington, MD 20895, USA.
Weiss
Deborah
D
Hudacik
Lauren
L
Restrepo
Susana
S
Galmin
Lindsey
L
Suschak
John
J
Draghia-Akli
Ruxandra
R
Markham
Phillip
P
Pal
Ranajit
R
D016915:AIDS Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000276:immunology; D018274:Electroporation / Q000379:methods*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D008252:Macaca fascicularis; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection / Q000379:methods; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology*
D016915:AIDS Vaccines; D000906:Antibodies; D019444:Vaccines, DNA