Design of recombinant antibody microarrays for cell surface membrane proteomics.
Generating proteomic maps of membrane proteins, common targets for therapeutic interventions and disease diagnostics, has turned out to be a major challenge. Antibody-based microarrays are among the novel rapidly evolving proteomic technologies that may enable global proteome analysis to be performed. Here, we have designed the first generation of a scaleable human recombinant scFv antibody microarray technology platform for cell surface membrane proteomics as well as glycomics targeting intact cells. The results showed that rapid and multiplexed profiling of the cell surface proteome (and glycome) could be performed in a highly specific and sensitive manner and that differential expression patterns due to external stimuli could be monitored.
Deptartment of Immunotechnology, BMC D13, Lund University, SE-221 84 Lund, Sweden.
Linda
L
Dexlin
Johan
J
Ingvarsson
Björn
B
Frendéus
Carl A K
CA
Borrebaeck
Christer
C
Wingren
D000906:Antibodies; D000954:Antigens, Surface / Q000032:analysis*; D004867:Equipment Design; D006801:Humans; D007128:Immunoglobulin Fragments; D008565:Membrane Proteins / Q000032:analysis; D040081:Protein Array Analysis; D040901:Proteomics / Q000379:methods*; D011994:Recombinant Proteins
D000906:Antibodies; D000954:Antigens, Surface; D007128:Immunoglobulin Fragments; D008565:Membrane Proteins; D011994:Recombinant Proteins; C055966:immunoglobulin Fv