Methylobacterium platani sp. nov., isolated from a leaf of the tree Platanus orientalis.
A novel bacterial strain, designated PMB02(T), was isolated from a leaf of the tree Platanus orientalis. Colonies grown on TYG agar plates were circular, pink-pigmented and slow-growing, being 0.2-1.5 mm in diameter after 3 days growth. The cells of strain PMB02(T) were Gram-negative, aerobic, motile rods that possessed oxidase and catalase activities and grew at 20-30 degrees C, pH 6-8 and in media containing less than 1 % NaCl. The major respiratory quinone was identified as Q-10. A phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain PMB02(T) was related to members of the genus Methylobacterium. A comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium aquaticum and Methylobacterium variabile, with which it showed sequence similarities of 97.7 and 97.4 %, respectively. The values for DNA-DNA hybridization between strain PMB02(T) and M. aquaticum CCM 7218(T) and M. variabile GR3(T) were less than 32 %. On the basis of the phenotypic characterization, the phylogenetic analysis and the DNA-DNA relatedness data, strain PMB02(T) is considered to represent a novel species of the genus Methylobacterium, for which the name Methylobacterium platani sp. nov. is proposed. The type strain is PMB02(T) (=KCTC 12901(T)=JCM 14648(T)).
Division of Environmental Science and Ecological Engineering, Korea University, Seoul, South Korea.
Yoon-Suk
YS
Kang
Division of Environmental Science and Ecological Engineering, Korea University, Seoul, South Korea.
Juhyun
J
Kim
Division of Environmental Science and Ecological Engineering, Korea University, Seoul, South Korea.
Hyeon-Dong
HD
Shin
Biological Resource Center, KRIBB, Daejeon, South Korea.
Young-Do
YD
Nam
Biological Resource Center, KRIBB, Daejeon, South Korea.
Jin-Woo
JW
Bae
Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, South Korea.
Che Ok
CO
Jeon
Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, South Korea.|Division of Environmental Science and Ecological Engineering, Korea University, Seoul, South Korea.
Woojun
W
Park
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D002374:Catalase / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D019684:Magnoliopsida / Q000382:microbiology*; D020580:Methylobacterium / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D018515:Plant Leaves / Q000382:microbiology*; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D010088:Oxidoreductases; D002374:Catalase