Application of biselective refocusing soft pulses to the simplification of heteronuclear correlation spectra.
The biselective spin echo technique allows the signals of coupled proton pairs to be extracted from crowded liquid state proton NMR spectra. Its use as a preparation sequence in heteronuclear chemical shift correlation experiments requires the removal of the heteronuclear coupling interaction during the biselective echo time. The discrimination between coupled and uncoupled protons signals is achieved by double quantum filtration, which delivers antiphase magnetization states. The latter are not directly compatible with the design of an HSQC-like pulse sequence. The conversion of antiphase to in-phase magnetization states by a second biselective echo sequence solves this problem. The optimization of spin echo delays is also discussed. Lastly, the article presents modified HSQC and HMBC pulses sequences in which information is obtained solely for the biselectively selected proton pairs. A peracetylated trisaccharide was used as a test molecule.
Centre National de la Recherche Scientifique, Institut de Chimie Moléculaire de Reims, REIMS Cedex 2, France. jm.nuzillard@univ-reims.fr
Jean-Marc
JM
Nuzillard
D016477:Artifacts; D002934:Cinnamates / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008962:Models, Theoretical; D008968:Molecular Conformation; D014312:Trisaccharides / Q000737:chemistry*
D002934:Cinnamates; D014312:Trisaccharides; C029010:cinnamic acid; C005190:melezitose