Diesel exhaust particle-exposed human bronchial epithelial cells induce dendritic cell maturation and polarization via thymic stromal lymphopoietin.
Human exposure to air pollutants, including ambient particulate matter, has been proposed as a mechanism for the rise in allergic disorders. Diesel exhaust particles, a major component of ambient particulate matter, induce sensitization to neoallergens, but the mechanisms by which sensitization occur remain unclear. We show that diesel exhaust particles upregulate thymic stromal lymphopoietin in human bronchial epithelial cells in an oxidant-dependent manner. Thymic stromal lymphopoietin induced by diesel exhaust particles was associated with maturation of myeloid dendritic cells, which was blocked by anti-thymic stromal lymphopoietin antibodies or silencing epithelial cell-derived thymic stromal lymphopoietin. Dendritic cells exposed to diesel exhaust particle-treated human bronchial epithelial cells induced Th2 polarization in a thymic stromal lymphopoietin-dependent manner. These findings provide new insight into the mechanisms by which diesel exhaust particles modify human lung mucosal immunity.
Department of Medicine, Division of Pulmonary and Critical Care Medicine, New York University School of Medicine, 550 1st Avenue, Room NB7N24, New York, NY, USA.
Bertram
B
Bleck
Doris B
DB
Tse
Maria A
MA
Curotto de Lafaille
Feijie
F
Zhang
Joan
J
Reibman
D000111:Acetylcysteine / Q000494:pharmacology; D000393:Air Pollutants / Q000494:pharmacology*; D000906:Antibodies / Q000494:pharmacology; D001980:Bronchi / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects* / Q000276:immunology; D002461:Cell Line, Transformed; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D018928:Immunity, Mucosal / Q000187:drug effects / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D010316:Particle Size; D034741:RNA, Small Interfering / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D001335:Vehicle Emissions; D000094632:Thymic Stromal Lymphopoietin
D000393:Air Pollutants; D000906:Antibodies; D016207:Cytokines; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; D001335:Vehicle Emissions; D013755:Tetradecanoylphorbol Acetate; D000111:Acetylcysteine; D000094632:Thymic Stromal Lymphopoietin