Transposon mediated transgenesis in a marine invertebrate chordate: Ciona intestinalis.
Achievement of transposon mediated germline transgenesis in a basal chordate, Ciona intestinalis, is discussed. A Tc1/mariner superfamily transposon, Minos, has excision and transposition activities in Ciona. Minos enables the creation of stable transgenic lines, enhancer detection, and insertional mutagenesis.
Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, 415-0025, Japan. sasakura@kurofune.shimoda.tsukuba.ac.jp
Yasunori
Y
Sasakura
Yuichi
Y
Oogai
Terumi
T
Matsuoka
Nori
N
Satoh
Satoko
S
Awazu
D000818:Animals; D002938:Ciona intestinalis / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D018014:Gene Transfer Techniques; D016678:Genome; D016254:Mutagenesis, Insertional
D004251:DNA Transposable Elements