Time course of peripheral oxidative stress as consequence of global ischaemic brain injury in rats.
Free radicals play an important role in the pathogenesis of brain injury. This study evaluates the potential relationship between ischaemia/reperfusion (I/R)-induced brain injury, peripheral oxidative stress (lymphocyte DNA damage), plasma antioxidant potential and uric acid levels. We observed that 15 min of ischaemia were sufficient to significantly increase lymphocyte DNA damage that remained elevated at the end of early (3 h) reperfusion and at later (72 h) reperfusion time; this parameter was not significantly increased, when compared to preoperated levels. In parallel, antioxidant potential was elevated after 15 min of ischaemia, remained high at early (3 h) reperfusion and decreased again with longer (72 h) reperfusion. A close association between the plasma antioxidant status and the uric acid content has been confirmed by findings that changes in TRAP values positively correlate with uric acid concentration in rat plasma after ischaemic injury. Moreover, results of in vitro experiments with extra uric acid addition to control plasma have shown that uric acid contributes to a greater part of TRAP values. These results indicate a similar time course of brain I/R-associated oxidative stress and peripheral antioxidant defence status and/or oxidative stress in animal experiments.
Jessenius Faculty of Medicine, Department of Medical Biochemistry, Comenius University, Malá hora 4, 036 01, Martin, Slovakia. sivonova@jfmed.uniba.sk
Monika
M
Sivonová
Peter
P
Kaplán
Zdenka
Z
Duracková
Dusan
D
Dobrota
Anna
A
Drgová
Zuzana
Z
Tatarková
Martina
M
Pavlíková
Erika
E
Halasová
Jan
J
Lehotský
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001930:Brain Injuries / Q000150:complications* / Q000473:pathology; D004249:DNA Damage / Q000502:physiology; D005609:Free Radicals / Q000097:blood / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications* / Q000473:pathology; D013997:Time Factors; D014527:Uric Acid / Q000097:blood
D000975:Antioxidants; D005609:Free Radicals; D014527:Uric Acid