Isolated spermatogonia protrude active pseudopodia in vitro.
When dispersed spermatogenic cells obtained by enzymatic digestion from prepuberal mice, adult male mice, nonazoospermic men and normospermic men were observed live using Normarski optics, it was found that, respectively, 47.4%, 1.4%, 5.1%, and 2.4% of them protruded active pseudopodia. These cells were 8 to 10 mum in diameter, had a high N/C ratio, and had one to two prominent nucleoli that were close to a distinct nuclear membrane. They showed low alkaline phosphatase activities and homogeneous nuclear immunoreactive patterns using gamma-H2AX, which suggests that they were spermatogonia.
Saint Mother Obstetrics and Gynecology Clinic and Institute for ART, Fukuoka, Japan. incho@stmother.com
Atsushi
A
Tanaka
Motoi
M
Nagayoshi
Shoichiro
S
Awata
Norio
N
Himeno
Izumi
I
Tanaka
Hiroshi
H
Kusunoki
D000328:Adult; D000818:Animals; D053713:Azoospermia / Q000473:pathology; D006801:Humans; D008297:Male; D051379:Mice; D011554:Pseudopodia / Q000648:ultrastructure*; D013093:Spermatogonia / Q000648:ultrastructure*