Selective isolation of bacterial DNA from human clinical specimens.
We evaluated two DNA preparation strategies (MolYsis, Molzym GmbH & Co. KG, Bremen, Germany) and Pureprove, SIRS-Lab GmbH, Jena, Germany) to selectively extract bacterial DNA from human clinical samples. By testing 16 oral samples we found that human DNA could be largely eliminated while detectable levels of bacterial DNA were obtained with all samples. Both approaches hold great potential for microbial diagnostic systems.
Horz
Hans-Peter
HP
Division of Oral Microbiology and Immunology, RWTH Aachen University Hospital, Germany. jhorz@ukaachen.de
Scheer
Sebastian
S
Huenger
Frank
F
Vianna
Morgana E
ME
Conrads
Georg
G
D000328:Adult; D001419:Bacteria / Q000302:isolation & purification; D016866:Bacteroidaceae Infections / Q000382:microbiology; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D003731:Dental Caries / Q000382:microbiology*; D006801:Humans; D010518:Periodontitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic; D013290:Streptococcal Infections / Q000382:microbiology; D013295:Streptococcus mutans / Q000235:genetics / Q000302:isolation & purification
D017931:DNA Primers; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic; D004247:DNA