Structural genomics of protein phosphatases.
The New York SGX Research Center for Structural Genomics (NYSGXRC) of the NIGMS Protein Structure Initiative (PSI) has applied its high-throughput X-ray crystallographic structure determination platform to systematic studies of all human protein phosphatases and protein phosphatases from biomedically-relevant pathogens. To date, the NYSGXRC has determined structures of 21 distinct protein phosphatases: 14 from human, 2 from mouse, 2 from the pathogen Toxoplasma gondii, 1 from Trypanosoma brucei, the parasite responsible for African sleeping sickness, and 2 from the principal mosquito vector of malaria in Africa, Anopheles gambiae. These structures provide insights into both normal and pathophysiologic processes, including transcriptional regulation, regulation of major signaling pathways, neural development, and type 1 diabetes. In conjunction with the contributions of other international structural genomics consortia, these efforts promise to provide an unprecedented database and materials repository for structure-guided experimental and computational discovery of inhibitors for all classes of protein phosphatases.
Almo
Steven C
SC
Albert Einstein College of Medicine, Bronx, NY, USA. almo@aecom.yu.edu
Bonanno
Jeffrey B
JB
Sauder
J Michael
JM
Emtage
Spencer
S
Dilorenzo
Teresa P
TP
Malashkevich
Vladimir
V
Wasserman
Steven R
SR
Swaminathan
S
S
Eswaramoorthy
Subramaniam
S
Agarwal
Rakhi
R
Kumaran
Desigan
D
Madegowda
Mahendra
M
Ragumani
Sugadev
S
Patskovsky
Yury
Y
Alvarado
Johnjeff
J
Ramagopal
Udupi A
UA
Faber-Barata
Joana
J
Chance
Mark R
MR
Sali
Andrej
A
Fiser
Andras
A
Zhang
Zhong-yin
ZY
Lawrence
David S
DS
Burley
Stephen K
SK
D000818:Animals; D018360:Crystallography, X-Ray; D023281:Genomics; D006801:Humans; D005810:Multigene Family; D010749:Phosphoprotein Phosphatases / Q000737:chemistry* / Q000145:classification / Q000235:genetics* / Q000502:physiology; D017422:Sequence Analysis, DNA
D010749:Phosphoprotein Phosphatases