Synthesis and evaluation of novel chromogenic peptidase substrates based on 9-(4'-aminophenyl)-10-methylacridinium salts as diagnostic tools in clinical bacteriology.
The synthesis and initial evaluation of novel chromogenic substrates with potential in the detection and differentiation of cultured bacterial colonies are described. The substrates were readily hydrolysed by specific aminopeptidase activity to release the chromogen, 9-(4'-aminophenyl)-10-methylacridinium salt, which provided a clear visual indication of the presence of the corresponding bacteria.
Anderson
Rosaleen J
RJ
Sunderland Pharmacy School, University of Sunderland, Sunderland SR1 3SD, UK. roz.anderson@sunderland.ac.uk
Groundwater
Paul W
PW
Huang
Yongxue
Y
James
Arthur L
AL
Orenga
Sylvain
S
Rigby
Annette
A
Roger-Dalbert
Céline
C
Perry
John D
JD
D000166:Acridines / Q000138:chemical synthesis / Q000737:chemistry*; D001424:Bacterial Infections / Q000175:diagnosis*; D010447:Peptide Hydrolases / Q000378:metabolism*; D013379:Substrate Specificity
D000166:Acridines; D010447:Peptide Hydrolases