[Simultaneous determination of 5 active components in Fructus Cnidii by HPLC].
OBJECTIVE
To develop an RP-HPLC method for simultaneous determination of 5 constituents in Fructus Cnidii.


METHODS
Analysis was performed on an Alltech C18 (4.6 mm x 250 mm, 5 microm) column. The mobile phases were acetonitrile water and acetic acid with gradient elution. The flow rate was 1 mL x min(-1). The monitoring wavelength was 325 nm and 245 nm. The column temperature was 40 degrees C.


RESULTS
The linear response ranges were 1-20 microg x mL(-1) (r = 0.999 9) for xanthotoxin, 1-20 microg x mL(-1) (r = 0.999 9) for isopimpinellin, 11-20 microg x mL(-1) (r = 0.999 8) for bergapten, 100-1 200 microg x mL(-1) (r = 0.999 7) for imperatorin, 100-2 000 microg x mL(-1) (r = 0.999 9) for osthole. The average recoveries were all above 95%.


CONCLUSIONS
The method is simple, sensitive and accurate with good reproducibility.
Zhejiang Deqing No. 3 People Hospital, Deqing 313201, China.
De-Xin
DX
Weng
Tian-Ao
TA
Li
D000078223:5-Methoxypsoralen; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031048:Cnidium / Q000737:chemistry*; D003374:Coumarins / Q000032:analysis; D005638:Fruit / Q000737:chemistry; D011564:Furocoumarins / Q000032:analysis*; D008730:Methoxsalen / Q000031:analogs & derivatives / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D015203:Reproducibility of Results
D003374:Coumarins; D011564:Furocoumarins; C015304:isopimpinellin; D000078223:5-Methoxypsoralen; C031534:imperatorin; D008730:Methoxsalen; C046627:osthol