Stereoselective determination of demethyl- and didemethyl-citalopram in rat plasma and brain tissue by liquid chromatography with fluorescence detection using precolumn derivatization.
A rapid and sensitive HPLC enantioselective method with fluorescence detection was developed to determine (-)-(R) and (+)-(S) enantiomers of the metabolites of citalopram, demethyl- and didemethyl-citalopram in plasma and brain tissue. This assay involves pre-column chiral derivatization with (-)-(R)-1-(1-naphthyl)ethyl isocyanate followed by separation on a normal-phase silica column. The developed liquid-liquid extraction procedure permits quantitative determination of analytes with recoveries ranged between 81 and 88% with intra- and inter-day relative standard deviations less than 10.5%. Linearity was obtained over the concentration range 5-1000 ng/mL and 100-10,000 ng/g for spiked drug-free plasma and brain tissue, respectively, with detection limits lower than 2.1 ng/mL and 42.8 ng/g.
Millán
S
S
Department of Analytical Chemistry, University of the Basque Country, Vitoria-Gasteiz, Spain.
Goicolea
M A
MA
Sánchez
A
A
Gómez-Caballero
A
A
Sampedro
M C
MC
Unceta
N
N
Barrio
R J
RJ
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015283:Citalopram / Q000097:blood / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D017367:Selective Serotonin Reuptake Inhibitors / Q000097:blood / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013237:Stereoisomerism
D015283:Citalopram; D017367:Selective Serotonin Reuptake Inhibitors