Glove-derived foreign proteins induce allergen-specific IgE in a mouse model.
Currently, most medical gloves are produced with a low content of natural rubber latex (NRL) protein. However, they may be substituted by proteins of foreign origin to maintain specific properties of the material. The aim of this study was to investigate the allergenicity and immunogenicity of unexpected proteins (i.e., soy and casein) compared with NRL proteins in a murine model in BALB/c mice. All respective allergen sources (extracts from three brands of NRL gloves, soy, and casein) were able to induce significant allergen-specific IgE and IgG(1) responses. On average, the highest IgE induction occurred after immunization with NRL, followed by soy and casein. Certain individuals from each treatment group exhibited levels of specific IgE as high as due to NRL. To analyze further specific IgE responses on a single allergen level, we established a microarray based on recombinant allergens for allergen-specific murine IgE detection. Besides specific IgE against rHev b 3, -6, -7, -8, and -11, specific IgE against kappa-casein could be detected in mice immunized with NRL glove extract, indicating a sensitization potential of the contained foreign protein. The substitution of genuine latex proteins by proteins of foreign origin may lead to a shift and de novo increase in sensitization to the finished products.
Department of Dermatology, University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.
Marion
M
Busch
Claudia
C
Schröder
Jens-Malte
JM
Baron
Hagen
H
Ott
Thomas
T
Bruckner
Thomas L
TL
Diepgen
Vera
V
Mahler
D000485:Allergens / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D002364:Caseins / Q000276:immunology; D005260:Female; D005932:Gloves, Surgical / Q000009:adverse effects*; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D040081:Protein Array Analysis; D011506:Proteins / Q000276:immunology* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D030262:Soybean Proteins / Q000276:immunology
D000485:Allergens; D002364:Caseins; D011506:Proteins; D011994:Recombinant Proteins; D030262:Soybean Proteins; D007073:Immunoglobulin E