Dynamics of proteins: light scattering study of dilute and dense colloidal suspensions of eye lens homogenates.
We report a dynamic light scattering study on protein suspensions of bovine lens homogenates at conditions (pH and ionic strength) similar to the physiological ones. Light scattering data were collected at two temperatures, 20 and 37 degrees C, over a wide range of concentrations from the very dilute limit up to the dense regime approaching the physiological lens concentration. A comparison with experimental data from intact bovine lenses was advanced, revealing differences between dispersions and lenses at similar concentrations. In the dilute regime, two scattering entities were detected and identified with the long-time self-diffusion modes of alpha-crystallins and their aggregates, which naturally exist in lens nucleus. Upon increasing protein concentration, significant changes in time correlation function were observed starting at approximately 75 mg ml(-1), where a new mode originating from collective diffusive motions becomes visible. Self-diffusion coefficients are temperature insensitive, whereas the collective diffusion coefficient depends strongly on temperature revealing a reduction of the net repulsive interparticle forces with decreasing temperature. While there are no rigorous theoretical approaches on particle diffusion properties for multicomponent, nonideal hard sphere polydispersed systems, as the suspensions studied here, a discussion of the volume fraction dependence of the long-time self-diffusion coefficient in the context of existing theoretical approaches was undertaken. This study is purported to provide some insight into the complex light scattering pattern of intact lenses and the interactions between the constituent proteins that are responsible for lens transparency. This would lead to understand basic mechanisms of specific protein interactions that lead to lens opacification (cataract) under pathological conditions.
Foundation for Research and Technology Hellas-Institute of Chemical Engineering and High Temperature Chemical Processes (FORTH/ICE-HT), Rio-Patras, Greece.
A
A
Giannopoulou
A J
AJ
Aletras
N
N
Pharmakakis
G N
GN
Papatheodorou
S N
SN
Yannopoulos
D000818:Animals; D002417:Cattle; D003102:Colloids / Q000737:chemistry*; D005136:Eye Proteins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007908:Lens, Crystalline / Q000737:chemistry*; D008027:Light; D008956:Models, Chemical; D009994:Osmolar Concentration; D012542:Scattering, Radiation; D013535:Suspensions / Q000737:chemistry*; D013997:Time Factors
D003102:Colloids; D005136:Eye Proteins; D013535:Suspensions