An enzymatic transglycosylation of purine bases.
An enzymatic transglycosylation of purine heterocyclic bases employing readily available natural nucleosides or sugar-modified nucleosides as donors of the pentofuranose fragment and recombinant nucleoside phosphorylases as biocatalysts has been investigated. An efficient enzymatic method is suggested for the synthesis of purine nucleosides containing diverse substituents at the C6 and C2 carbon atoms. The glycosylation of N(6)-benzoyladenine and N(2)-acetylguanine and its O(6)-derivatives is not accompanied by deacylation of bases.
Department of Pharmaceutical Chemistry, University of Kuopio, Kuopio, Finland.
Jarkko
J
Roivainen
Tatiana
T
Elizarova
Seppo
S
Lapinjoki
Igor A
IA
Mikhailopulo
Roman S
RS
Esipov
Anatoly I
AI
Miroshnikov
D006031:Glycosylation; D015394:Molecular Structure; D011684:Purine Nucleosides / Q000096:biosynthesis / Q000737:chemistry; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism*; D011687:Purines / Q000737:chemistry* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013816:Thermodynamics
D011684:Purine Nucleosides; D011687:Purines; D011994:Recombinant Proteins; D011683:Purine-Nucleoside Phosphorylase