Genotype of bovine sterol regulatory element binding protein-1 (SREBP-1) is associated with fatty acid composition in Japanese Black cattle.
To investigate genetic factors that affect fatty acid composition in beef carcass, we previously investigated genetic profiles of stearoyl-CoA desaturase (SCD) and their effect on fatty acid composition in fat tissue of cattle. It has been known that sterol regulatory element binding protein (SREBP) is a transcription factor that regulates gene expression levels of SCD and other genes relevant to lipid and fatty acid metabolism in tissue. Therefore, we determined the full-length sequence of bovine SREBP-1 cDNA and then surveyed polymorphisms in whole exons and introns in the bovine genome. Large 84-bp insertion (long type: L) and deletion (short type: S) were found in intron 5 of bovine SREBP-1 in Japanese Black cattle, although there was no notable mutation in exon regions. The associations between the SREBP-1 genotypes and fatty acid compositions/fat melting points were analyzed by using genomic DNA with carcass trait information from 606 Japanese Black cattle. The S type contributed to 1.3% higher monounsaturated fatty acid (MUFA) proportion and 1.6 degrees C lower melting point in intramuscular fat. Genotyping of bovine SREBP-1 is considered to reflect a genetic variation which is associated with physiologic characteristics of fat tissue in Japanese black cattle.
Laboratory of Animal Breeding & Genetics, Graduate School of Agricultural Science, Kobe University, Nada, Kobe, 657-8501, Japan.
Shogo
S
Hoashi
Nobuhisa
N
Ashida
Hideki
H
Ohsaki
Takeshi
T
Utsugi
Shinji
S
Sasazaki
Masaaki
M
Taniguchi
Kenji
K
Oyama
Fumio
F
Mukai
Hideyuki
H
Mannen
D000273:Adipose Tissue / Q000033:anatomy & histology / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D017124:Conserved Sequence; D017931:DNA Primers; D005227:Fatty Acids / Q000032:analysis*; D005838:Genotype; D006801:Humans; D007564:Japan; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics*
D017931:DNA Primers; D005227:Fatty Acids; D051780:Sterol Regulatory Element Binding Protein 1