A rapid apoptosis assay measuring relative acridine orange fluorescence in zebrafish embryos.
The ability to easily analyze apoptosis is important in studies of molecular cell biology and to evaluate the relative toxicity of different treatments or environments. This is particularly the case when substances such as morpholino oligonucleotides are injected into embryos, as such treatments can cause widespread, complex patterns of apoptosis. Zebrafish embryos are well suited for cell biological and environmental toxicity analyses, but the need remains for a simple method that can analyze levels of apoptosis in a statistically significant number of embryos. Here we present a "group fluorescence" method for rapid, large-scale analysis of relative levels of apoptosis based on densitometric techniques.
Tucker
Ben
B
ARC Special Research Center for the Molecular Genetics of Development and Discipline of Genetics, School of Molecular and Biomedical Science, The University of Adelaide, Adelaide, Australia. ben.tucker@adelaide.edu.au
Lardelli
Michael
M
D000165:Acridine Orange / Q000032:analysis / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D003720:Densitometry / Q000379:methods / Q000662:veterinary*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000502:physiology*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage; D013194:Staining and Labeling / Q000379:methods*; D015027:Zebrafish / Q000502:physiology*
D005456:Fluorescent Dyes; D016376:Oligonucleotides, Antisense; D000165:Acridine Orange