Loss of actin does not affect export of newly synthesized proteins to the surface of Trypanosoma brucei.
Vesicle traffic to and from the surface is highly polarized in African trypanosomes. Actin is required for polarized endocytic traffic in bloodstream forms of African trypanosomes but its role in other pathways has remained equivocal. A combination of metabolic pulse chase labelling and surface biotinylation during the chase period along with the use of conditional RNA interference was employed to demonstrate that substantial loss of actin had no effect on the export of newly synthesized proteins to the surface of bloodstream and procyclic forms of Trypanosoma brucei. These results indicated that this trafficking pathway to the surface operates as normal even when actin levels are significantly lower than normal and endocytic activity is abolished. Taken together the data support the view that the secretory and endocytic pathways are not obligatorily coupled.
School of Biochemistry and Immunology, Trinity College Dublin, Ireland. denolan@tcd.ie
Derek P
DP
Nolan
Jose A
JA
Garcia-Salcedo
D000199:Actins / Q000235:genetics / Q000502:physiology*; D000818:Animals; D020868:Gene Silencing; D021381:Protein Transport; D015800:Protozoan Proteins / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D000199:Actins; D015800:Protozoan Proteins