Interaction of reactive oxygen and nitrogen species with albumin- and methemoglobin-bound dinitrosyl-iron complexes.
Destructive effect of superoxide anions O2- derived from KO(2) or xanthine-xanthine oxidase system on dinitrosyl-iron complexes bound with bovine albumin or methemoglobin (DNIC-BSA or DNIC-MetHb) was demonstrated. The sensitivity of DNIC-BSA synthesized by the addition of DNIC with cysteine, thiosulfate or phosphate (DNIC-BSA-1, DNIC-BSA-2 or DNIC-BSA-3, respectively) to destructive action of O2- decreased in row: DNIC-BSA-1>DNIC-BSA-3>DNIC-BSA-2. The estimated rate constant for the reaction between O2- and DNIC-BSA-3 was equal to approximately 10(7)M(-1)s(-1). However, hydrogen peroxide and tert-butyl hydrogenperoxide (t-BOOH) did not induce any noticeable degradation of DNIC-BSA-3 even when used at concentrations exceeding by one order of magnitude those of the complex. As to their action on DNIC-MetHb both hydrogen peroxide and t-BOOH-induced rapid degradation of the complex. Both agents could induce the process due to the effect of alkylperoxyl or protein-derived free radicals formed at the interaction of the agents with ferri-heme groups of MetHb. Peroxynitrite (ONOO(-)) could also initiate protein-bound DNIC degradation more efficiently in the reaction with DNIC-BSA-3. Higher resistance of DNIC-MetHb to peroxynitrite was most probably due to the protective action of heme groups on ONOO(-). However, the analysis allows to suggest that the interaction of protein-bound DNICs with O2- is the only factor responsible for the degradation of the complexes in cells and tissues.
Shumaev
Konstantin B
KB
Russian Cardiological Research-and-Production Complex, Moscow, Russian Federation.
Gubkin
Andrey A
AA
Serezhenkov
Vladimir A
VA
Lobysheva
Irina I
II
Kosmachevskaya
Olga V
OV
Ruuge
Enno K
EK
Lankin
Vadim Z
VZ
Topunov
Alexey F
AF
Vanin
Anatoly F
AF
D000818:Animals; D002417:Cattle; D006861:Hydrogen Peroxide / Q000737:chemistry; D007501:Iron / Q000737:chemistry*; D008706:Methemoglobin / Q000737:chemistry*; D009589:Nitrogen Oxides / Q000737:chemistry*; D030421:Peroxynitrous Acid / Q000737:chemistry; D026361:Reactive Nitrogen Species / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013997:Time Factors; D020122:tert-Butylhydroperoxide / Q000737:chemistry
D009589:Nitrogen Oxides; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D030421:Peroxynitrous Acid; D012710:Serum Albumin, Bovine; C073661:dinitrosyl iron complex; D008706:Methemoglobin; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D007501:Iron