[Determination and analysis of toluene diisocyanate metabolites in mice using gas chromatography-mass spectrometry].
In the present research we used gas chromatography-mass spectrometry (G C-MS) to determine metabolites of toluene diisocyanate (TDI) in mice and deduce the pathway for toluene diisocyanate metabolism in the organism. Conditions for TDI chromatography: Supelco PTETM-5 chromatographic column (30 mm x 0.25 mm x 0.25 microm); initial column temperature: 90 degrees C, which was maintained for 30 min, then the temperature was increased at a rate of 40 degrees C x min(-1) to 280 degrees C, and maintained for 5.25 min; temperature for the vaporizing chamber: 250 degrees C; carrier gas: helium flowing at 1.0 microL x min(-1). Conditions for chromatography of TDI metabolites in the organism: 94% methyl, 4% ethenyl-bonded-phase fused-silica capillary column (30 + 2 m x 0.25 + 0.02 mm); initial column temperature: 30 degrees C, which was maintained for 5 min, after and then was increased at a rate of 80 degrees C x min(-1) to 280 degrees C, and maintained for 5 min; temperature for the vaporizing chamber: 250 degrees C; carrier gas: helium flowing at 1.0 microL x min(-1). Conditions for mass spectrometry: EI for ionization; 70 eV for ionization energy; 280 degrees C for connecting tube temperature; 35-350 micro for range of scanning; and 1.0 microL for sample size. The results showed that 2 ,4-toluene diisocyanate was metabolized into 2,4-diaminotoluene. Under the conditions selected for GC-MS, TDI metabolites in the organism can be isolated and identified.
Center of Research and Development of Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China.
Yu-Bin
YB
Ji
Chen-Feng
CF
Ji
Xiang
X
Zou
Hui-Xin
HX
Liu
D000818:Animals; D004785:Environmental Pollutants / Q000032:analysis* / Q000097:blood / Q000378:metabolism* / Q000652:urine; D005243:Feces / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014051:Toluene 2,4-Diisocyanate / Q000032:analysis* / Q000097:blood / Q000378:metabolism* / Q000652:urine
D004785:Environmental Pollutants; D014051:Toluene 2,4-Diisocyanate