Black rice (Oryza sativa L. var. japonica) hydrolyzed peptides induce expression of hyaluronan synthase 2 gene in HaCaT keratinocytes.
Black rice (Oryza sativa L. var. japonica) has been used in folk medicine in Asia. To understand the effects of black rice hydrolyzed peptides (BRP) from germinated black rice, we assessed the expression levels of about 20,000 transcripts in BRP-treated HaCaT keratinocytes using human 1A oligo microarray analysis. As a result, the BRP treatment showed a differential expression ratio of more than 2-fold: 745 were activated and 1,011 were repressed. One of the most interesting findings was a 2-fold increase in hyaluronan synthase 2 (HAS2) gene expression by BRP. Semiquantitative RT-PCR showed that BRP increased HAS2 mRNA in dose-dependent manners. ELISA showed that BRP effectively increased hyaluronan (HA) production in HaCaT keratinocytes.
Sim
Gwan Sub
GS
R&D Center Hanbul Cosmetics Co. Ltd., Chungbuk 369-834, Korea.
Lee
Dong-Hwan
DH
Kim
Jin-Hwa
JH
An
Sung-Kwan
SK
Choe
Tae-Boo
TB
Kwon
Tae-Jong
TJ
Pyo
Hyeong-Bae
HB
Lee
Bum-Chun
BC
D002478:Cells, Cultured; D020869:Gene Expression Profiling; D014453:Glucuronosyltransferase / Q000235:genetics*; D006801:Humans; D000076002:Hyaluronan Synthases; D006868:Hydrolysis; D015603:Keratinocytes / Q000201:enzymology*; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000737:chemistry*; D010940:Plant Proteins / Q000494:pharmacology*
D010940:Plant Proteins; D014453:Glucuronosyltransferase; C101754:HAS2 protein, human; D000076002:Hyaluronan Synthases