Detection of methicillin resistance in Staphylococcus aureus isolates using two-step triplex PCR and conventional methods.
A two-step triplex PCR assay targeting the mecA, femA, and nuc genes was developed for the detection of methicillin resistance genes harbored by some Staphylococcus aureus isolates and for the simultaneous identification of such isolates at the species level. The triplex PCR revealed the presence of the femA and nuc genes in all the S. aureus isolates examined (n=105). Forty-four clinical isolates were mecA positive and no foodborne isolates were mecA positive. The PCR results had a 98 or 99% correlation with the results of PBP2a latex agglutination tests or oxacillin susceptibility tests, respectively.
Cho
Joon-Il
JI
Department of Food Science and Technology, Chung-Ang University, Anseong 456-756, Korea.
Jung
Hye-Jin
HJ
Kim
Young-Joon
YJ
Park
Sung-Hee
SH
Ha
Sang-Do
SD
Kim
Keun-Sung
KS
D001426:Bacterial Proteins / Q000235:genetics; D004720:Endonucleases / Q000235:genetics; D016106:Methicillin Resistance / Q000235:genetics*; D008836:Micrococcal Nuclease / Q000235:genetics; D046915:Penicillin-Binding Proteins; D016133:Polymerase Chain Reaction / Q000379:methods*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics
D001426:Bacterial Proteins; C096686:FemA protein, Bacteria; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus; C074002:nuc protein, staphylococcus; D004720:Endonucleases; D008836:Micrococcal Nuclease