Parenteral long-acting amoxicillin reduces intestinal bacterial community diversity in piglets even 5 weeks after the administration.
We investigated the long-term effects of a single intramuscular administration of amoxicillin (15 mg kg(-1)) 1 day after birth, on piglet intestinal microbiota. Animals received no creep feed before weaning on day 28 of age. For the next 11 days, the piglets received a wheat-barley-based diet. Colon digesta samples were collected on day 39 and subjected to denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene fragments. DGGE fingerprint diversity indices differed between the group treated with amoxicillin and the untreated group (0.8+/-0.19 and 1.03+/-0.17, respectively, P=0.012). Reamplification and sequencing of two bands present in all samples revealed that a Roseburia faecalis-related population was strongly reduced in relative abundance (98% identity) in the treated group, while an enterobacterial population with 100% identity to Shigella spp., Escherichia coli and Salmonella enterica serovar Typhi was enriched. A band corresponding to Lactobacillus sobrius was present only in the control group. The protective effect of prophylactic antibiotic administration may be outweighed by the long-lasting disturbance of the gut ecosystem.
Research Unit for Nutritional Physiology Oskar Kellner, Research Institute for the Biology of Farm Animals, Dummerstorf, Germany.
Pawel
P
Janczyk
Robert
R
Pieper
Wolfgang Bernhard
WB
Souffrant
Diane
D
Bimczok
Hermann-Josef
HJ
Rothkötter
Hauke
H
Smidt
D000658:Amoxicillin / Q000008:administration & dosage / Q000494:pharmacology*; D000821:Animal Feed; D000818:Animals; D000833:Animals, Suckling; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D004269:DNA, Bacterial / Q000032:analysis; D017753:Ecosystem; D004591:Electrophoresis, Polyacrylamide Gel; D007273:Injections, Intramuscular; D007422:Intestines / Q000187:drug effects / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013552:Swine
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D000658:Amoxicillin