A role for the inositol kinase Ipk1 in ciliary beating and length maintenance.
Cilia project from cells as membranous extensions, with microtubule structural cores assembling from basal bodies by intraflagellar transport (IFT). Here, we report a ciliary role for the inositol 1,3,4,5,6-pentakisphosphate 2-kinase (Ipk1) that generates inositol hexakisphosphate. In zebrafish embryos, reducing Ipk1 levels inhibited ciliary beating in Kupffer's vesicle and decreased ciliary length in the spinal canal, pronephric ducts, and Kupffer's vesicle. Electron microscopy showed that ciliary axonemal structures were not grossly altered. However, coincident knockdown of Ipk1 and IFT88 or IFT57 had synergistic perturbations. With GFP-Ipk1 enriched in centrosomes and basal bodies, we propose that Ipk1 plays a previously uncharacterized role in ciliary function.
Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232-8240, USA.
Bhaskarjyoti
B
Sarmah
Virginia P
VP
Winfrey
Gary E
GE
Olson
Bruce
B
Appel
Susan R
SR
Wente
D000818:Animals; D030801:Animals, Genetically Modified; D001692:Biological Transport; D019521:Body Patterning; D002460:Cell Line; D018385:Centrosome / Q000378:metabolism; D002923:Cilia / Q000201:enzymology* / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008870:Microtubules / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
D015800:Protozoan Proteins; D029961:Zebrafish Proteins; D017853:Phosphotransferases (Alcohol Group Acceptor); C503314:ippk protein, zebrafish