Mechanical alterations of rabbit Achilles' tendon after immobilization correlate with bone mineral density but not with magnetic resonance or ultrasound imaging.
OBJECTIVE
To assess the usefulness of magnetic resonance imaging (MRI), ultrasound (US) imaging, or bone mineral density (BMD) in predicting the mechanical properties of immobilized rabbit Achilles' tendons.


METHODS
Experimental study.


METHODS
Basic university laboratory.


METHODS
Twenty-eight rabbits.


METHODS
Twelve rabbits had 1 hindlimb casted for 4 weeks and 10 rabbits were casted for 8 weeks. Contralateral legs and 12 normal hindlimbs served as controls.


METHODS
Achilles' tendon dimensions on MRI and US, T1- and T2-signal intensities on MRI, classification of abnormalities on MRI and US; BMD of the calcaneus with dual-energy x-ray absorptiometry. Biomechanic measures consisted of peak load, stiffness, and stress. Imaging variables were correlated with biomechanic alterations.


RESULTS
Immobilized Achilles' tendons were weaker and showed decreased mechanical stress compared with their contralateral legs and controls (all P<.05). MRI and US revealed larger Achilles' tendons after immobilization. However, neither increased MRI nor US signal abnormality was found. BMD was lower in immobilized calcanei and larger in contralateral legs than controls. Only BMD correlated with both the decreased peak load (R2=.42, P<.05) and stress (R2=.54, P<.05) of immobilized Achilles' tendon.


CONCLUSIONS
This study established weakened mechanical properties of immobilized Achilles' tendons. BMD of the calcaneus, but not MRI and US, was predictive of the mechanical alterations in immobilized Achilles' tendons. BMD may be a useful biomarker to monitor disease and recovery in Achilles' tendons.
Trudel
Guy
G
Department of Medicine, Bone and Joint Research Laboratory, University of Ottawa, Ottawa, ON, Canada. gtrudel@ottawahospital.on.ca <gtrudel@ottawahospital.on.ca>
Koike
Yoichi
Y
Ramachandran
Nanthan
N
Doherty
Geoff
G
Dinh
Laurent
L
Lecompte
Martin
M
Uhthoff
Hans K
HK
D000125:Achilles Tendon / Q000000981:diagnostic imaging / Q000503:physiopathology*; D000818:Animals; D001696:Biomechanical Phenomena; D015519:Bone Density; D005260:Female; D007103:Immobilization / Q000009:adverse effects*; D008279:Magnetic Resonance Imaging; D011817:Rabbits; D014463:Ultrasonography