In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography.
We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in opaque tissues. We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time.
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th Street, Charlestown, Massachusetts 02129, USA. cvinegoni@mgh.harvard.edu
Claudio
C
Vinegoni
Chrysoula
C
Pitsouli
Daniel
D
Razansky
Norbert
N
Perrimon
Vasilis
V
Ntziachristos
D000818:Animals; D004331:Drosophila melanogaster / Q000166:cytology*; D007089:Image Enhancement / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008029:Lighting / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D011679:Pupa / Q000166:cytology; D041622:Tomography, Optical / Q000379:methods*