Influence of GSTM1 null and low repair XPC PAT+ on anti-B[a]PDE-DNA adduct in mononuclear white blood cells of subjects low exposed to PAHs through smoking and diet.
The influence of low-activity NER genotypes (XPC PAT-/+, XPA-A23G, XPD Asp312Asn, XPD Lys751Gln) and GSTM1 (active or null) was evaluated on anti-benzo[a]pyrene diol epoxide-(B[a]PDE)-DNA adduct formed in the lymphocyte plus monocyte fraction (LMF). The sample population consisted of 291 healthy subjects with low exposure to polycyclic aromatic hydrocarbons (PAHs) (B[a]P) through their smoking (n=126 smokers) or dietary habits (n=165 non-smokers with high (>or=52 times/year) consumption of charcoaled meat or pizza). The bulky anti-B[a]PDE-DNA adduct levels were detected by HPLC/fluorescence analysis and genotypes by PCR. Anti-B[a]PDE-DNA was present (>or=0.5 adducts/10(8) nucleotides) in 163 (56%) subjects (median (range) 0.77 (0.125-32.0) adducts/10(8) nucleotides), with smokers showing a significantly higher adduct level than non-smokers with high consumption of PAH-rich meals (P<0.01). Our exposed-sample population with unfavourable XPC PAT+/- or +/+ and GSTM1 null genotypes has the significantly highest adduct level (P<0.01). Taking into account tobacco smoke and diet as sources of exposure to B[a]P, low-activity XPC PAT+ shows a major role in smokers (P<0.05) and GSTM1 null in non-smokers with frequent consumption of PAH-rich meals (P<0.01). The modulation of anti-B[a]PDE-DNA adduct in the LMF by GSTM1 null and low-activity XPC PAT+ polymorphisms may be considered as potential genetic susceptibility factors that can modify individual responses to low PAH (B[a]P) genotoxic exposure, with the consequent risk of cancer in the general population.
Occupational Health Section, Department of Environmental Medicine and Public Health, University of Padova, Via Giustiniani 2, 35128 Padova, Italy. sofia.pavanello@unipd.it
Sofia
S
Pavanello
Alessandra
A
Pulliero
Erminio
E
Clonfero
D000217:Acyltransferases / Q000235:genetics*; D000328:Adult; D018736:DNA Adducts; D004268:DNA-Binding Proteins / Q000235:genetics*; D004032:Diet / Q000009:adverse effects*; D005260:Female; D020022:Genetic Predisposition to Disease; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D000071518:Liver X Receptors; D008214:Lymphocytes / Q000000981:diagnostic imaging; D008297:Male; D008875:Middle Aged; D057093:Orphan Nuclear Receptors; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D011110:Polymorphism, Genetic; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics; D012907:Smoking / Q000009:adverse effects*; D014463:Ultrasonography
D000217:Acyltransferases; D018736:DNA Adducts; D004268:DNA-Binding Proteins; D005982:Glutathione Transferase; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D011084:Polycyclic Aromatic Hydrocarbons; D018160:Receptors, Cytoplasmic and Nuclear; C086984:XPC protein, human; C105599:HHAT protein, human