[Duration of viral RNA circulation in blood of patients with hepatitis A].
Duration of hepatitis A virus (HAV) RNA circulation in blood of patients with HA was assessed and compared with intensity of cytolytic syndrome. Detection of viral RNA was performed by RT-PCR method with specific primers to VP1/P2A region of HAV genome. 54 blood serum samples from 40 patients were prospectively studied on the presence of HAV RNA. The latterwas detected in 53.7% of serum samples. The greatest number of positive results of HAV RNA detection in blood of the patients with HA was obtained from 8th to 21st day of illness (77.4%). Prolonged viremia (42+/-9 days) was observed in more than 20% of the patients. The maximal time of HAV RNA daetection in blood serum amounted 74 days (period of follow-up). HAV RNA was present in almost all patients with AIAT activity higher than 500 U/l regardless of duration of illness.
Gorchakova
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OV
Esaulenko
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Mukomolov
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D000410:Alanine Transaminase / Q000097:blood; D017931:DNA Primers; D006506:Hepatitis A / Q000097:blood / Q000821:virology*; D017957:Hepatitis A Virus, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000097:blood / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors; D015678:Viral Structural Proteins / Q000235:genetics; D014766:Viremia
D017931:DNA Primers; D012367:RNA, Viral; C096539:VP1 protein, hepatitis A virus; D015678:Viral Structural Proteins; D000410:Alanine Transaminase