Homo-dimerization of RyR1 C-terminus via charged residues in random coils or in an alpha-helix.
To investigate the mechanism by which the C-terminus (4,938-5,037) of the ryanodine receptor 1 (RyR1) homo-tetramerizes, forming a functional Ca(2+)-release channel, the structural requirements for the tetramerization were studied using site-directed mutagenesis. Alanine-substitutions at five charged residues, E4976, H5003, D5026, E5033 and D5034, significantly decreased the formation of homo-dimers (reduced by >50%). Interaction between the C-terminus and cytoplasmic loop I (4,821-4,835) required two positively charged residues, H4832 and K4835. Based on the predicted protein secondary structures, all seven charged residues are located in random coils. Paired alanine-substitutions at six negatively charged residues (E4942A/D4953A, D4945A/E4952A and E4948A/ E4955A) of the alpha-helix (4,940-4,956) in the C-terminus increased homo-dimerization. Therefore, the homo-tetramerization of RyR1 may be mediated by intra- and/or inter-monomer electrostatic interactions among the C-terminal charged residues in random coils or in an alpha-helix.
Department of Physiology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea. EHUI@catholic.ac.kr
Eun Hui
EH
Lee
Paul D
PD
Allen
D000595:Amino Acid Sequence; D000818:Animals; D019281:Dimerization; D066298:In Vitro Techniques; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D019837:Ryanodine Receptor Calcium Release Channel / Q000737:chemistry* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D055672:Static Electricity
D011993:Recombinant Fusion Proteins; D019837:Ryanodine Receptor Calcium Release Channel