Preparation and properties of collagen/modified hyaluronic acid hydrogel for biomedical application.
Hydrogels composed of collagen and hyaluronic acid are types of crosslinked water-swellable polymers and possess vast potential for applications in the medical industry. Collagen (Co) is the major structural protein of connective tissues such as skin, tendon and cartilage. Hyaluronic acid (HA) is a non-immunogenic, non-adhesive glycosaminoglycan that has a high water absorption property and plays significant roles in several cellular processes. The purpose of this study is to prepare a collagen (Co)-modified hyaluronic acid (MHA) hydrogel and investigate its potential utility for biomedical products such as wound dressing materials. Collagen (Co, type I) was obtained from pig skin and mucopolysaccharide-HA was modified by a poly (ethylene glycol) diglycidyl ether (PEGDGE) crosslinker. Thermal stability, swelling behavior, and mechanical strength of Co-MHA hydrogel according to different mass ratios of Co and MHA in hydrogel networks were investigated. The physical properties of the hydrogel were measured by SEM, Differential Scanning Calorimetry (DSC), Thermal Gravity Analysis (TGA), and a Universal Testing Machine (UTM). The cell viability of Co-MHA hydrogel was also evaluated using an in vitro MTT assay.
Kim
Jae-Kyung
JK
Department of Chemical Engineering, Inha University, Incheon 402-751, Korea.
Lee
Jung-Soo
JS
Jung
Hyo-Jung
HJ
Cho
Jin-Hui
JH
Heo
Jee-In
JI
Chang
Yoon-Ho
YH
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage* / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D024042:Collagen Type I / Q000008:administration & dosage* / Q000737:chemistry* / Q000648:ultrastructure; D003460:Crystallization / Q000379:methods; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000737:chemistry*; D020100:Hydrogels / Q000008:administration & dosage / Q000737:chemistry; D046911:Macromolecular Substances / Q000008:administration & dosage / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D011817:Rabbits; D013499:Surface Properties
D001672:Biocompatible Materials; D024042:Collagen Type I; D020100:Hydrogels; D046911:Macromolecular Substances; D006820:Hyaluronic Acid