Cloning and characterization of a PI-like MADS-box gene in Phalaenopsis orchid.
The highly evolved flowers of orchids have colorful sepals and fused columns that offer an opportunity to discover new genes involved in floral development in monocotyledon species. In this investigation, we cloned and characterized the homologous PISTALLATA-like (PI-like) gene PhPI15 (Phalaenopsis PI STILLATA # 15), from the Phalaenopsis hybrid cultivar. The protein sequence encoded by PhPI15 contains a typical PI-motif. Its sequence also formed a subclade with other monocot PI-type genes in phylogenetic analysis. Southern analysis showed that PhPI15 was present in the Phalaenopsis orchid genome as a single copy. Furthermore, it was expressed in all the whorls of the Phalaenopsis flower, while no expression was detected in vegetative organs. The flowers of transgenic tobacco plants ectopically expressing PhPI15 showed male-sterile phenotypes. Thus, as a Class-B MADS-box gene, PhPI15 specifies floral organ identity in orchids.
Institute of Genetics, Research Center of Gene Diversity and Designed Agriculture, Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Science, Fudan University, Shanghai 200433, China.
Bin
B
Guo
Saiyin
S
Hexige
Tian
T
Zhang
Jon K
JK
Pittman
Donghong
D
Chen
Feng
F
Ming
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D035264:Flowers / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005801:Genes, Homeobox; D017343:Genes, Plant; D017403:In Situ Hybridization; D026161:MADS Domain Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D029595:Orchidaceae / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics / Q000254:growth & development
D017931:DNA Primers; D018744:DNA, Plant; D026161:MADS Domain Proteins; D010940:Plant Proteins