Gene expression profiling during Forskolin induced differentiation of BeWo cells by differential display RT-PCR.
The differentiation of cytotrophoblasts into syncytiotrophoblasts in the placenta has been employed as a model to investigate stage specific expression as well as regulation of genes during this process. While the cytotrophoblasts are highly invasive and proliferative with relatively less capacity to synthesize pregnancy related proteins, the multinucleated syncytiotrophoblasts are non-proliferative and non-invasive. However, syncytiotrophoblasts are the site of synthesis of a variety of protein, peptide and steroid hormones as well as several growth factors. Both the freshly isolated cytotrophoblasts from human placenta as well as the BeWo cell, a choriocarcinoma cell line model which retain several characteristic of cytotrophoblasts has been employed by us to study regulation of differentiation. In the present study, we have employed the differential display RT-PCR analysis (DD-RT-PCR) to evaluate gene expression changes during Forskolin induced in vitro differentiation of BeWo cells. We have identified several genes which are differentially expressed during differentiation and the differential expression of 10 transcripts was confirmed by Northern blot analysis. Based on the identity of the transcripts an attempt has been made to relate the known function of the gene products, to changes observed during differentiation. Of the several transcripts, one of the transcripts, namely Secretory Leukocyte Protease Inhibitor (SLPI) which is known to have multiple functions was found to increase 15-fold in the syntiotrophoblast.
Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
P S
PS
Neelima
A J
AJ
Rao
D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics*; D045744:Cell Line, Tumor; D002822:Choriocarcinoma / Q000235:genetics / Q000473:pathology; D005576:Colforsin / Q000494:pharmacology*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014594:Uterine Neoplasms / Q000235:genetics / Q000473:pathology
D012333:RNA, Messenger; D005576:Colforsin