Growth and genetic responses of Salmonella Typhimurium to pH-shifts in an anaerobic continuous culture.
Salmonella infection of chickens that leads to potential human foodborne salmonellosis continues to be a concern. Changes in the pH of poultry gastrointestinal tract could influence Salmonella growth and virulence response. In the current study, growth responses of a chicken isolate Salmonella enterica serovar Typhimurium (ST) to three incremental pH-shifts (6.17-7.35) in continuous cultures (CC) were evaluated. The expression of rpoS and hilA was determined by real time-polymerase chain reaction (RT-PCR) as well. Increases in pH resulted in higher cell protein concentrations, glucose disappearance, and glucose and ATP yields. Although with some inconsistency between the two trials, the data indicated that the ammonia release into media was favored by low pH. The pH shifts did not significantly affect acetate biosynthesis. No consistent trends of pH influence on propionate and butyrate production could be detected. In all three pH shifts, relative expression of hilA was dominant at 0h which represented CC steady state. In pH shift 7.35-6.86 (Trial 1), the relative expression of rpoS at time 0 and 1h were over five-fold higher than after 3 and 6h of growth. Overall, the results suggest that ST physiology is altered by changes in pH, which could be determinant factors for ST survival in the poultry gastrointestinal ecosystems.
Dunkley
K D
KD
Department of Poultry Science, Texas A&M University, College Station, TX 77843-2472, USA.
Callaway
T R
TR
Chalova
V I
VI
Anderson
R C
RC
Kundinger
M M
MM
Dunkley
C S
CS
Nisbet
D J
DJ
Ricke
S C
SC
D000693:Anaerobiosis; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002645:Chickens / Q000382:microbiology*; D005227:Fatty Acids / Q000096:biosynthesis; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D012478:Salmonella Food Poisoning; D012486:Salmonella typhimurium / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D005227:Fatty Acids; C102236:HilA protein, Salmonella; D012808:Sigma Factor; D015534:Trans-Activators; C069567:sigma factor KatF protein, Bacteria