Determination of water-soluble polyphenolic compounds in commercial herbal teas from Lamiaceae: peppermint, melissa, and sage.
Chromatographic techniques (HPLC and HPTLC) were used for qualitative and quantitative determination of eriocitrin, luteolin 7-O-rutinoside, luteolin 7-O-beta-glucuronide, lithospermic acid, rosmarinic acid, and methyl rosmarinate together with other known compounds in commercial herbal teas from the Lamiaceae family: peppermint leaf (Menthae piperitae folium), melissa leaf (Melissae folium), and sage leaf (Salviae officinalis folium). Contents of analyzed compounds in infusions, the most popular forms, were established using a C18 column with acetonitrile-water-formic acid as a mobile phase. The HPLC method was validated for linearity, precision, and accuracy. Luteolin 7-O-beta-glucuronide and lithospermic acid were identified as new Mentha x piperita compounds. The investigated herbal teas delivered polyphenols in high amounts, up to 182.2 mg for the infusion of one peppermint tea bag.
Department of Pharmacognosy, Wrocław Medical University, Poland. izabela@farmgn.am.wroc.pl
Izabela
I
Fecka
Sebastian
S
Turek
D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D031334:Melissa / Q000737:chemistry*; D036142:Mentha piperita / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols; D027543:Salvia officinalis / Q000737:chemistry*; D012995:Solubility; D014867:Water
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D014867:Water