Binding of Leptospira to extracellular matrix proteins.
BACKGROUND
Leptospirosis is a zoonotic disease of global importance. Pathogenesis caused by this infectious disease remains unclear. Attachment of pathogenic leptospires to host tissues is a crucial initial step to establish the infection.


OBJECTIVE
Study the binding of the spirochete to three types of extracellular matrix (ECM), collagen type IV, fibronectin, and laminin, which are major components of target organs.


METHODS
ELISA-based experiments were performed to determine binding of pathogenic (serovar icterohaemorrhagie) and non-pathogenic (serovar Patoc) serovars, to purified ECM.


RESULTS
Both pathogenic and non-pathogenic serovars bound to all three types of ECM in the dose-dependent manner and the binding to fibronectin is higher than to collagen and laminin (p < 0.005).


CONCLUSIONS
Pathogenic leptospires can bind to various types of ECM and the binding of leptospires to fibronectin was higher than to collagen and laminin. However, this capability may not be the only mechanism that makes leptospires virulent since non-pathogenic leptospire can bind the ECM as well.
Chirathaworn
Chintana
C
Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. chintana_chula@yahoo.com
Patarakul
Kanittha
K
Saksit
Vuttisak
V
Poovorawan
Yong
Y
D003094:Collagen; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D006801:Humans; D007797:Laminin; D007921:Leptospira interrogans; D007922:Leptospirosis / Q000382:microbiology* / Q000503:physiopathology / Q000635:transmission; D010865:Pilot Projects; D013144:Spirochaetales; D017741:Survivors
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D003094:Collagen