[Effects of short-term continuous shear stress on cells growth and recovery of Laminaria japonica gametophytic cells in photobioreactor].
Laminaria japonica gametophytic cells were cultivated in a photobioreactor under continuous shear stress (0-1000 r/min) in 60 hours and the following static cultivation within 23.5 days. The content of chlorophyll a reached the maximum value of 2.36 mg/L at the end of continuous shear stress when the agitation speed was 90 r/min, while the chlorophyll a (chl a) concentration decreased quickly and nitrogen and phosphorus were released under high shear force (270-1000 r/min). The cell injury ratio at 1000r/min was as 18 times as that of the control. During the recovery course, gametophytic cells showed themselves distinct recovery capability at all agitation speeds. Furthermore, the content of chl a is a more exact index as biomass than dry cells weight (DCW). Besides cell injury ratio, the liberation of phosphorus demonstrates the cells injury.
Center of Cell Culture and Bioprocess Engineering, Shanghai Jiaotong University, Shanghai 200240, China.
Na
N
Huang
Si-Ye
SY
Chen
Han-Shi
HS
Qi
D019149:Bioreactors; D018929:Cell Culture Techniques; D005718:Gametogenesis / Q000502:physiology*; D005854:Germ Cells / Q000502:physiology*; D007795:Laminaria / Q000254:growth & development* / Q000528:radiation effects; D008027:Light; D033081:Shear Strength; D013314:Stress, Mechanical