Glycosylation of flavonoids with E. coli expressing glycosyltransferase from Xanthomonas campestris.
Glycosyltransferase family 1 (UGT) uses small chemicals including phenolics, antibiotics, and alkaloids as substrates to have an influence in biological activities. A glycosyltransferase (XcGT-2) from Xanthomonas campestris was cloned and consisted of a 1,257 bp open reading frame encoding a 45.5 kDa protein. In order to use this for the modification of phenolic compounds, XcGT-2 was expressed in Escherichia coli as a glutathione S-transferase fusion protein. With the E. coli transformant expressing XcGT-2, biotransformation of flavonoids was carried out. Flavonoids having a double bond between carbons 2 and 3, and hydroxyl groups at both C-3' and C-4', were glycosylated and the glycosylation position was determined to be at the hydroxyl group of C-3', using nuclear magnetic resonance spectroscopy. These results showed that XcGT-2 regiospecifically transferred a glucose molecule to the 3'-hydroxyl group of flavonoids containing both 3' and 4'-hydroxyl groups.
Kim
Ho Jeong
HJ
Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Korea.
Kim
Bong Gyu
BG
Kim
Jae Ah
JA
Park
Younghee
Y
Lee
Yoon Jung
YJ
Lim
Yoongho
Y
Ahn
Joong-Hoon
JH
D001709:Biotechnology / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism*; D047311:Luteolin / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D011794:Quercetin / Q000737:chemistry / Q000378:metabolism; D016959:Xanthomonas campestris / Q000201:enzymology* / Q000235:genetics
D005419:Flavonoids; D011794:Quercetin; D016695:Glycosyltransferases; D047311:Luteolin