Tailor-made fructooligosaccharides by a combination of substrate and genetic engineering.
The combination of sucrose analogues as novel substrates (substrate engineering) and highly active recombinant beta-fructofuranosidase from A. niger (genetic engineering) provides a new powerful tool for the efficient preparative synthesis of tailor-made saccharides of the important 1-kestose and 1-nystose type headed with different monosaccharides of interest. These novel compounds have been isolated. They did not display toxic effects or suppress cell growth in initial studies, making these new compounds potential candidates for prebiotics.
Department of Microbiology, Technical University of Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany.
Alga
A
Zuccaro
Sven
S
Götze
Susanne
S
Kneip
Petra
P
Dersch
Jürgen
J
Seibel
D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology; D005818:Genetic Engineering; D006801:Humans; D007700:Kinetics; D009005:Monosaccharides / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology; D009844:Oligosaccharides / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D014312:Trisaccharides / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology; D043324:beta-Fructofuranosidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D009005:Monosaccharides; D009844:Oligosaccharides; D011994:Recombinant Proteins; D014312:Trisaccharides; C116580:fructooligosaccharide; C072433:1-kestose; D043324:beta-Fructofuranosidase