Role of galK and galM in galactose metabolism by Streptococcus thermophilus.
Streptococcus thermophilus is unable to metabolize the galactose moiety of lactose. In this paper, we show that a transformant of S. thermophilus SMQ-301 expressing Streptococcus salivarius galK and galM was able to grow on galactose and expelled at least twofold less galactose into the medium during growth on lactose.
Vaillancourt
Katy
K
Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, Quebec, Canada G1K 7P4.
Bédard
Nathalie
N
Bart
Christian
C
Tessier
Mélanie
M
Robitaille
Gilles
G
Turgeon
Nathalie
N
Frenette
Michel
M
Moineau
Sylvain
S
Vadeboncoeur
Christian
C
D015152:Blotting, Northern; D015153:Blotting, Western; D002238:Carbohydrate Epimerases / Q000235:genetics* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D017931:DNA Primers / Q000235:genetics; D005686:Galactokinase / Q000235:genetics* / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D048249:Streptococcus thermophilus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014169:Transformation, Bacterial
D003470:Culture Media; D017931:DNA Primers; D005686:Galactokinase; D002238:Carbohydrate Epimerases; C459183:galactose mutarotase; D005690:Galactose