Agnostic particle tracking for three-dimensional motion of cellular granules and membrane-tethered bead dynamics.
The ability to detect biological events at the single-molecule level provides unique biophysical insights. Back-focal-plane laser interferometry is a promising technique for nanoscale three-dimensional position measurements at rates far beyond the capability of standard video. We report an in situ calibration technique for back-focal-plane, low-power (nontrapping) laser interferometry. The technique does not rely on any a priori model or calibration knowledge, hence the name "agnostic". We apply the technique to track long-range (up to 100 microm) motion of a variety of particles, including magnetic beads, in three-dimensions with high spatiotemporal resolution ( approximately 2 nm, 100 micros). Our tracking of individual unlabeled vesicles revealed a previously unreported grouping of mean-squared displacement curves at short timescales (<10 ms). Also, tracking functionalized magnetic beads attached to a live cell membrane revealed an anchorage-dependent nonlinear response of the membrane. The software-based technique involves injecting small perturbations into the probe position by driving a precalibrated specimen-mounting stage while recording the quadrant photodetector signals. The perturbations and corresponding quadrant photodetector signals are analyzed to extract the calibration parameters. The technique is sufficiently fast and noninvasive that the calibration can be performed on-the-fly without interrupting or compromising high-bandwidth, long-range tracking of a particle.
Desai
Kalpit V
KV
Department of Biomedical Engineering, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. kvdesai@gmail.com
Bishop
T Gary
TG
Vicci
Leandra
L
O'Brien
E Timothy
ET
Taylor
Russell M
RM
Superfine
Richard
R
D000818:Animals; D001703:Biophysics / Q000295:instrumentation / Q000379:methods*; D002138:Calibration; D002462:Cell Membrane / Q000378:metabolism; D003584:Cytological Techniques; D004058:Diffusion; D004548:Elasticity; D004867:Equipment Design; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D007700:Kinetics; D007834:Lasers; D008566:Membranes / Q000378:metabolism*; D018715:Microscopy, Video; D008954:Models, Biological