In vivo functions of the gamma-butyrolactone autoregulator receptor in Streptomyces ambofaciens producing spiramycin.
A gene encoding a gamma-butyrolactone autoregulator receptor was cloned in to E. coli from Streptomyces ambofaciens producing spiramycin, a macrolide antibiotic used in both veterinary medicine and human medicine. A 714-bp intact receptor gene (saaR) was obtained by PCR and genomic Southern hybridization with the 100-bp PCR product as a probe. To clarify the in vivo function of saaR, a saaR-disrupted strain was constructed by means of homologous recombination, and phenotypes were compared with those of the wild-type strain. The number of saaR-disruptant spores was 4-fold less than that of the wild-type strain. In addition, saaR deletion from the S. ambofaciens chromosome resulted in complete loss of spiramycin production suggesting that saaR is a rare positive regulator, controlling both spiramycin biosynthesis and sporulation.
Choi
Sun-Uk
SU
Department of Food Science and Biotechnology, Kyungnam University, Masan, 631-701, Republic of Korea.
Kim
Mi-Kyung
MK
Ha
Heon-Su
HS
Hwang
Yong-Il
YI
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011963:Receptors, GABA-A / Q000235:genetics*; D011995:Recombination, Genetic; D015572:Spiramycin / Q000096:biosynthesis*; D013171:Spores, Bacterial / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D013997:Time Factors
C037481:4-butyrolactone receptor; D001426:Bacterial Proteins; D011963:Receptors, GABA-A; D015572:Spiramycin