On-line sample preconcentration in capillary electrophoresis. Fundamentals and applications.
On-line preconcentration is one of the aspects of analytical method development using capillary electrophoretic techniques. The choice of the sample matrix alone can significantly alter both method sensitivity and separation efficiency. The recent trend to detect samples in narrower separation vessels also necessitates the need to improve detection sensitivity. The desire to detect very low levels of analytes using limited amounts of sample from biological specimens and the high separation efficiency obtainable using very large injections compared to classical small size injections also adds to this list. Indeed, one of the rich areas of research in the capillary electrophoresis field is on on-line sample preconcentration. More than 400 published research articles gathered from the http://www.webofscience.com from the year 2000 described a form of on-line preconcentration in capillary electrophoresis. This review provides a comprehensive table listing the applications of on-line preconcentration in capillary electrophoresis.
University of California, Berkeley, School of Public Health, Berkeley, CA 94720, USA.
Steven L
SL
Simpson
Joselito P
JP
Quirino
Shigeru
S
Terabe
D000818:Animals; D001688:Biological Products / Q000032:analysis; D054750:Ecotoxicology / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods; D005504:Food Analysis / Q000379:methods; D053593:Forensic Toxicology / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009862:Online Systems; D004364:Pharmaceutical Preparations / Q000032:analysis
D001688:Biological Products; D004364:Pharmaceutical Preparations