Cytoplasmic calcium increases in response to changes in the gravity vector in hypocotyls and petioles of Arabidopsis seedlings.
Plants respond to a large variety of environmental signals, including changes in the gravity vector (gravistimulation). In Arabidopsis (Arabidopsis thaliana) seedlings, gravistimulation is known to increase the cytoplasmic free calcium concentration ([Ca(2+)](c)). However, organs responsible for the [Ca(2+)](c) increase and the underlying cellular/molecular mechanisms remain to be solved. In this study, using Arabidopsis seedlings expressing apoaequorin, a Ca(2+)-sensitive luminescent protein in combination with an ultrasensitive photon counting camera, we clarified the organs where [Ca(2+)](c) increases in response to gravistimulation and characterized the physiological and pharmacological properties of the [Ca(2+)](c) increase. When the seedlings were gravistimulated by turning 180 degrees, they showed a transient biphasic [Ca(2+)](c) increase in their hypocotyls and petioles. The second peak of the [Ca(2+)](c) increase depended on the angle but not the speed of rotation, whereas the initial peak showed diametrically opposite characters. This suggests that the second [Ca(2+)](c) increase is specific for changes in the gravity vector. The potential mechanosensitive Ca(2+)-permeable channel (MSCC) inhibitors Gd(3+) and La(3+), the Ca(2+) chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA), and the endomembrane Ca(2+)-permeable channel inhibitor ruthenium red suppressed the second [Ca(2+)](c) increase, suggesting that it arises from Ca(2+) influx via putative MSCCs in the plasma membrane and Ca(2+) release from intracellular Ca(2+) stores. Moreover, the second [Ca(2+)](c) increase was attenuated by actin-disrupting drugs cytochalasin B and latrunculin B but not by microtubule-disrupting drugs oryzalin and nocodazole, implying that actin filaments are partially involved in the hypothetical activation of Ca(2+)-permeable channels. These results suggest that the second [Ca(2+)](c) increase via MSCCs is a gravity response in the hypocotyl and petiole of Arabidopsis seedlings.
Toyota
Masatsugu
M
Department of Physiology, Nagoya University Graduate School of Medicine, Showa-ku, Nagoya, Aichi 466-8550, Japan.
Furuichi
Takuya
T
Tatsumi
Hitoshi
H
Sokabe
Masahiro
M
D017360:Arabidopsis / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D006112:Gravitation; D018515:Plant Leaves / Q000166:cytology* / Q000378:metabolism; D036226:Seedlings / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors
D002118:Calcium