Interaction proteome analysis of Xanthomonas Hrp proteins.
Because of the importance of the type III protein-secretion system in bacteria-plant interaction, its function in bacterial pathogenesis of plants has been intensively studied. To identify bacterial proteins interacting with Xanthomonas hrp gene products that are involved in pathogenicity, we performed the glutathione-bead binding analysis of Xanthomonas lysates containing GST-tagged Hrp proteins. Analysis of glutathione-bead bound proteins by 1-DE and MALDI-TOF has demonstrated that Avr proteins, RecA, and several components of the type III secretion system interact with HrpB protein. This proteomic approach could provide a powerful tool in finding interaction partners of Hrp proteins whose roles in host-pathogen interaction need further studies.
Proteome Research Laboratory, KRIBB, Daejeon 305-333, Korea.
Mi
M
Jang
Byoung Chul
BC
Park
Do Hee
DH
Lee
Kwang-Hee
KH
Bae
Sayeon
S
Cho
Hyun Seok
HS
Park
Baek Rak
BR
Lee
Sung Goo
SG
Parki
D001426:Bacterial Proteins / Q000032:analysis*; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014974:Xanthomonas / Q000737:chemistry* / Q000235:genetics* / Q000472:pathogenicity
D001426:Bacterial Proteins; D020543:Proteome