Epithelial: lamina propria lymphocyte interactions promote epithelial cell differentiation.
OBJECTIVE
Intestinal lymphoepithelial interactions occur in the epithelium and the subepithelial space. We asked whether normal, Crohn's disease (CD), or ulcerative colitis (UC) lamina propria lymphocytes (LPL) could promote intestinal epithelial cell (IEC) growth and differentiation.


METHODS
T84 cells were cocultured with isolated LPL. IECs were then lysed and subjected to measurement of intestinal alkaline phosphatase (IAP) activity; Western blot analysis for MAPK and Akt activation; and real-time polymerase chain reaction to assess caudal-related homeoprotein 2 (CDX2) messenger RNA (mRNA) levels. Tissue sections were immunostained for evidence of mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) activation, CDX2, and IAP; and CDX2 mRNA expression was assessed in human colonic biopsy specimens.


RESULTS
IAP activity was increased in T84 cells cocultured for 8 days with normal LPL (P < .05) and even greater with CD LPL (P < .001). Crypt IECs in active CD mucosa expressed IAP ex vivo. Phospho-MAPK (extracellular signal-regulated kinase 1/2, p38, and c-Jun-N-terminal kinase) and phospho-Akt were seen as early as 30 minutes after coculture. MAPK activation was greatest in T84 cells cocultured with CD LPL. There was a specific increase in Phospho-p38 MAPK and Phospho-Akt staining in the nuclei of crypt IECs in active vs inactive CD, normal mucosa, and UC mucosa. CDX2 mRNA expression was increased in CD LPL cocultured T84 cells, which did not correlate with CDX2 protein localization ex vivo.


CONCLUSIONS
There is cross talk between LPL and IECs, which leads to IEC differentiation. The differentiation is accelerated in CD mucosa.
Dahan
Stephanie
S
Immunology Institute, Mount Sinai School of Medicine, New York, New York, USA.
Roda
Giulia
G
Pinn
David
D
Roth-Walter
Franziska
F
Kamalu
Okebugwu
O
Martin
Andrea P
AP
Mayer
Lloyd
L
D000469:Alkaline Phosphatase; D000951:Antigens, Neoplasm / Q000378:metabolism; D000071616:CDX2 Transcription Factor; D002450:Cell Communication / Q000502:physiology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000166:cytology*; D058851:GPI-Linked Proteins; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000378:metabolism / Q000473:pathology*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology*; D008214:Lymphocytes / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism
D000951:Antigens, Neoplasm; D000071616:CDX2 Transcription Factor; C491270:CDX2 protein, human; D058851:GPI-Linked Proteins; D018398:Homeodomain Proteins; D012333:RNA, Messenger; C063088:ALPI protein, human; D000469:Alkaline Phosphatase