Combination of HSCCC and Sephadex LH-20 methods An approach to isolation and purification of the main individual theaflavins from black tea.
In order to separate the main individual theaflavin monomers from black tea, high-speed countercurrent chromatography (HSCCC) and Sephadex LH-20 column chromatography were applied. The results showed that theaflavin (TF1), theaflavin-3-gallate (TF2A), theaflavin-3'-gallate (TF2B) and theaflavin-3,3'-digallate (TF3) can be obtained by HSCCC using a solvent system composed of n-hexane-ethyl acetate-methanol-water (1:3:1:6, v/v/v/v), but the TF1 was containing epicatechin-3-gallate (ECG). Similarly, Sephadex LH-20 can also effectively separate TF2A(B) and TF3, but epigallocatechin-3-gallate (EGCG) contaminated TF1, too. Combination of HSCCC and Sephadex LH-20, the preferably purified TF1, TF2A(B) and TF3 were obtained than single separation technique. In addition, ECG and EGCG were also suggested to be able to be comprehensively separated by combination of the two techniques.
Key Laboratory of Tea Biochemistry & Biotechnology, Ministry of Agriculture & Ministry of Education, Anhui Agricultural University, Hefei, Anhui 230036, China.
Changjun
C
Yang
Daxiang
D
Li
Xiaochun
X
Wan
D044946:Biflavonoids / Q000737:chemistry / Q000302:isolation & purification*; D002392:Catechin / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D013662:Tea / Q000737:chemistry*
D044946:Biflavonoids; D010936:Plant Extracts; D013662:Tea; C056068:theaflavin; C088983:theaflavine gallate; D005707:Gallic Acid; D002392:Catechin