Piezoelectric immunosensor for the direct and rapid detection of Francisella tularensis.
A novel immunosensing device based on a piezoelectric sensor for direct detection of the biological warfare agent Francisella tularensis was developed. This sensor includes mouse polyclonal antibody immobilized in a layer of protein A covalently linked to the gold electrode of the sensor. The immunosensor is able to detect F. tularensis with the limit of detection 10(5) CFU/mL with a typical measuring cycle > 5 min. The sensor was successfully evaluated for rapid detection of F. tularensis spikes in drinking water and milk; no deterioration of sensitivity in comparison with buffer solutions was observed. The proposed concept of a rapid measurement of microbial agents seems to be promising for evaluation of samples after short pre-cultivation enrichment.
Pohanka
M
M
Center of Biological Defense, 561 66 Tĕchonín, Czechia.
Skládal
P
P
D000818:Animals; D000907:Antibodies, Bacterial / Q000737:chemistry*; D054045:Biological Warfare Agents; D015374:Biosensing Techniques / Q000379:methods*; D005260:Female; D005604:Francisella tularensis / Q000302:isolation & purification*; D051379:Mice; D008807:Mice, Inbred BALB C; D014406:Tularemia / Q000382:microbiology*
D000907:Antibodies, Bacterial; D054045:Biological Warfare Agents