Protein expression diversity amongst serovars of Salmonella enterica.
Salmonella enterica is one of the most extensively studied bacterial species in terms of physiology, genetics, cell culture and development. As a very diverse group, the serovars of S. enterica display a spectrum of host specificities ranging from a broad host range to strictly host-adapted variants. This study utilized a classic proteomic approach combining 2D gel electrophoresis and mass spectrometry for the comparative analysis of the proteomes of serovars Typhimurium, Enteritidis, Choleraesuis, Pullorum and Dublin. The comparative analysis revealed species-specific protein factors with no significant change in expression amongst all isolates, as well as proteins with fluctuating expression levels between serovars and strains. Examples include an isoform of SodA specific for serovar Typhimurium, the third isoform of the lysine arginine ornithine (LAO)-binding amino acid transporter specific for serovar Pullorum, and the enzyme GabD found to be unique to serovar Choleraesuis. Overall the study demonstrated the importance of using multiple isolates when characterizing the expression patterns of bacteria in order to account for the intrinsic diversity of a bacterial population and revealed several factors with potential roles in host adaptation and pathogenicity of the serovars of S. enterica.
Encheva
V
V
Molecular Identification Services Unit, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5EQ, UK.
Wait
R
R
Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College, ARC Building, 1 Aspenlea Road, London W6 8LH, UK.
Begum
S
S
Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College, ARC Building, 1 Aspenlea Road, London W6 8LH, UK.
Gharbia
S E
SE
Applied and Functional Genomics Unit, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5EQ, UK.
Shah
H N
HN
Molecular Identification Services Unit, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5EQ, UK.
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D013058:Mass Spectrometry; D040901:Proteomics; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D012703:Serotyping; D013045:Species Specificity
D001426:Bacterial Proteins