A novel mimovirus vaccine containing survivin epitope with adjuvant IL-15 induces long-lasting cellular immunity and high antitumor efficiency.
Numerous evidences have indicated that CD8+ cytotoxic T lymphocytes (CTLs) played a significant role in protecting host against tumors, thus CTLs have been involved as potentially relevant candidate targets of cancer immunotherapy. Moreover it is crucial to fully elucidate antigen-specific CTL response, especially high-avidity and long-lasting CTL response in vivo. In the present study, we reported a novel tumor vaccine (mimovirus), a viron-size particulate which consisted of a cell-penetrating peptide of Tat(49-57), a CTL epitope peptide survivin(85-93) and a plasmid encoding murine interleukin-15 (IL-15). We demonstrated that this tumor vaccine could effectively mediate the expression of the gene and presentation of the tumor antigen derived peptide ex vivo. Furthermore, the tumor vaccine supported a robust memory CTL-mediated long-term immunity in vivo, which could effectively protect BALB/c mice against fatal CT26 tumor challenge and improve their survival. These findings suggest that the tumor vaccine may provide an alternative therapeutic strategy for cancer patients.
Yang
Zhao
Z
Institute of Immunology, Third Military Medical University, Ave. Gaotanyan 30th, District Shapingba, Chongqing 400038, People's Republic of China.
Wang
Li
L
Wang
Huiming
H
Shang
Xiaoyun
X
Niu
Wei
W
Li
Jintao
J
Wu
Yuzhang
Y
D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D000939:Epitopes; D005260:Female; D015696:Gene Products, tat / Q000276:immunology*; D007111:Immunity, Cellular; D051034:Inhibitor of Apoptosis Proteins; D019409:Interleukin-15 / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008869:Microtubule-Associated Proteins / Q000276:immunology*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000276:immunology*; D009368:Neoplasm Transplantation; D010446:Peptide Fragments / Q000276:immunology; D010957:Plasmids / Q000276:immunology; D000077022:Survivin; D022223:Vaccines, Subunit / Q000276:immunology
C107489:BIRC5 protein, human; D019496:Cancer Vaccines; D000939:Epitopes; D015696:Gene Products, tat; D051034:Inhibitor of Apoptosis Proteins; D019409:Interleukin-15; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D000077022:Survivin; D022223:Vaccines, Subunit