Neutralization of hemorrhagic snake venom metalloproteinase HR1a from Protobothrops flavoviridis by human monoclonal antibody.
Human monoclonal antibodies (HuMAbs) against HR1a from Protobothrops (formerly Trimeresurus) flavoviridis venom were obtained by the fusion of SP2/0-Ag14 myeloma cells and spleen cells from KM mice immunized with purified HR1a. The ability of HuMAbs to neutralize the HR1a was determined by in vitro neutralization assay and by neutralization of the hemorrhagic activity. The initial screening of over 300 hybridoma fusion wells resulted in the establishment of 80 HR1a-reactive hybridomas. Of the reactive clones, HuMAb HR1a-7 and HR1a-18 neutralized both proteolytic and hemorrhagic activity of HR1a. Mapping of epitope recognized by the reactive clones was performed by using an ELISA that measured antibody binding to overlapping peptides (15 amino acid peptide offset frameshifted by three residues) covering the metalloproteinase domain sequence of HR1a. HuMAbs HR1a-7 and HR1a-18 neutralized HR1a by reacting with peptides of EQQRYLNNFRFIELV and IVNTLNETYRYL. The three-dimensional structure of HR1a based on a homology modeling predicted that these two epitopes are surface exposed.
Morine
Nobuya
N
Department of Biological Sciences, Okinawa Prefectural Institute of Health and Environment, 2085 Ozato, Nanjo-city, Okinawa 901-1202, Japan. morinenb@pref.okinawa.lg.jp
Matsuda
Seiko
S
Terada
Koki
K
Eto
Akira
A
Ishida
Isao
I
Oku
Hirosuke
H
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D018604:Epitope Mapping; D006801:Humans; D006825:Hybridomas; D008297:Male; D045726:Metalloproteases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D011487:Protein Conformation; D014757:Viper Venoms / Q000037:antagonists & inhibitors / Q000201:enzymology* / Q000276:immunology; D017819:Viperidae
D000911:Antibodies, Monoclonal; D014757:Viper Venoms; D045726:Metalloproteases