Proangiogenic potential of a collagen/bioactive glass substrate.
OBJECTIVE
Previous attempts to stimulate angiogenesis have focused on the delivery of growth factors and cytokines, genes encoding for specific angiogenic inductive proteins or transcription factors, or participating cells. While high concentrations of bioactive glasses have exhibited osteogenic potential, recent studies have demonstrated that low concentrations of particular bioactive glasses are angiogenic. We hypothesized that a well known bioactive glass (Bioglass 45S5) possesses proangiogenic potential over a limited range of concentrations.


METHODS
Varying amounts of Bioglass were loaded into absorbable collagen sponges. The proangiogenic potential of Bioglass was determined by examining the capacity of the soluble products to induce endothelial cell proliferation, tubule formation in a co-culture, and upregulate vascular endothelial growth factor (VEGF) production.


RESULTS
We determined a range of Bioglass concentrations which exhibit proangiogenic potential. Furthermore, we demonstrated that the proangiogenic capacity of this material is related to the soluble dissolution products of Bioglass and the subsequent production of cell-secreted angiogenic factors by stimulated cells.


CONCLUSIONS
These studies suggest that this bioactive glass possesses a robust proangiogenic potential, and this strategy may provide an alternative to recombinant inductive growth factors.
Department of Biomedical Engineering, University of California Davis, Davis, CA 95616, USA.
Ann
A
Leu
J Kent
JK
Leach
D043924:Angiogenesis Modulating Agents / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D018920:Coculture Techniques; D003094:Collagen; D042783:Endothelial Cells / Q000187:drug effects; D005898:Glass; D006801:Humans; D008870:Microtubules / Q000187:drug effects / Q000648:ultrastructure; D008934:Mitogens / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000254:growth & development; D018919:Neovascularization, Physiologic / Q000502:physiology*; D010006:Osteoblasts / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D054457:Tissue Scaffolds; D015854:Up-Regulation / Q000187:drug effects; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000235:genetics
D043924:Angiogenesis Modulating Agents; D008934:Mitogens; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D003094:Collagen