Immunolocalization of a novel collectin CL-K1 in murine tissues.
We have recently identified a novel collectin, CL-K1, that may play a role in innate immunity as a member of the collectin family. In this study using mice, we investigated the tissue distribution of CL-K1 for better understanding of its pathophysiological relevance. Real-time PCR analyses demonstrated that CL-K1 mRNA was expressed in all tissues tested. Immunohistochemical analyses demonstrated that CL-K1 was expressed in proximal tubules of kidney, in mucosa of the gastrointestinal tract, and in bronchial glands of bronchioles similar to the localization of SP-A and SP-D in these pulmonary structures. Immunohistochemistry also showed that CL-K1 was highly expressed in hepatocytes around the central veins in liver, which suggests that murine CL-K1 may be mainly produced in the liver and secreted into the blood stream as is human CL-K1. CL-K1 was especially detected in vascular smooth muscle in several types of tissues. In addition, it was also expressed in intestinal Paneth cells, in mesangial cells of kidney, in pancreatic islet D cells, and in neurons of the brain. It is of interest that this profile of CL-K1 expression is unique among the collectins. Together these histological findings may be useful for understanding the biological function of this novel collectin.
Department of Microbiology and Immunochemistry, Asahikawa Medical College, 2-1-1-1Midorigaoka-Higashi, Asahikawa 078-8510, Japan. momu@asahikawa-med.ac.jp
Wataru
W
Motomura
Takayuki
T
Yoshizaki
Katsuki
K
Ohtani
Toshikatsu
T
Okumura
Mituko
M
Fukuda
Jun
J
Fukuzawa
Kenichiro
K
Mori
Seong-Jae
SJ
Jang
Naoki
N
Nomura
Itsuro
I
Yoshida
Yasuhiko
Y
Suzuki
Yutaka
Y
Kohgo
Nobutaka
N
Wakamiya
D000818:Animals; D000906:Antibodies; D016466:CHO Cells; D037222:Collectins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits
D000906:Antibodies; C518521:Colec11 protein, human; D037222:Collectins; D012333:RNA, Messenger