[Genetic polymorphism of FSH b subunit gene and correlation with reproductive traits in Beijing Black Pig].
FSH beta subunit gene was regarded as a candidate gene for reproductive traits of Beijing Black Pig in this study. The polymorphism of two loci FSHbeta-1 and FSHbeta-2 was detected by electrophoretic method and PCR-RFLP with restriction endonuclease Hae III. Sequencing results showed that a 273 bp sequence, which was a retrotransponsons including a RNA Polymerase III inter promoter, was inserted between the 134th and 135th nucleotide of the PCR product in FSHbeta-1, and the mutation (C-->T) was revealed at the 173th nucleotide of the PCR product in FSHbeta-2. Both alleles (A and B) of both loci were found in the population that showed low polymorphism. Chi-square test indicated that the two polymorphism sites fitted Hardy-Weinberg equilibrium (P>0.05). The effects of polymorphism of FSH b subunit gene on total born number (TNB), number born alive (NBA), and birth weight (WB) were analyzed. For FSHbeta-1 locus, pigs of the first parity with genotype AA had 0.96 and 1.85 TNB more than those with genotypes AB and BB. The pigs of the first parity with genotypes AA and AB had 0.95 and 1.69 NBA more than those with genotype BB, respectively. For FSHbeta-2 locus, pigs of the first parity with genotype AA had1.57 and 2.15 TNB more than those with genotypes AB and BB. The pigs of the first parity with genotypes AA and AB had 1.00 and 0.94 NBA more than those with genotype BB, respectively. The pigs of multiparous with genotype AA had 0.25 kg of WB more than those with genotype BB. The results of combined genotype effects indicated that A allele of FSHbeta-1 locus in all the population and FSHbeta-2 locus in pigs of the first parity had the positive effective on TNB, NBA, and WB.
Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100094, China. luorenzhuoma@163.com
Zhuoma
Z
Luoreng
Li-Xian
LX
Wang
Shi-Duo
SD
Sun
D000818:Animals; D001483:Base Sequence; D001724:Birth Weight; D004262:DNA Restriction Enzymes / Q000378:metabolism; D037201:Follicle Stimulating Hormone, beta Subunit / Q000235:genetics* / Q000378:metabolism; D005787:Gene Frequency; D005838:Genotype; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012098:Reproduction / Q000235:genetics*; D016415:Sequence Alignment; D013552:Swine / Q000235:genetics* / Q000502:physiology*
D037201:Follicle Stimulating Hormone, beta Subunit; D004262:DNA Restriction Enzymes