Functional analysis of CD8+ T cell responses to the onconeural self protein cdr2 in patients with paraneoplastic cerebellar degeneration.
Paraneoplastic cerebellar degeneration (PCD) is linked to an immune response against cerebellar degeneration related antigen 2 (cdr2) co-expressed in tumor and Purkinje neurons. Here, comprehensive immune-assessment assays were used to analyze CD8(+) T cells from 7 PCD patients, but no evidence was found of CD8(+) T cells specific for either of two previously described cdr2 epitopes (cdr2-1 and cdr2-2). In contrast, viral-specific CD8(+) T cells from healthy volunteers and PCD patients were measurable. These findings are inconsistent with an obligate role for cdr2-1- or cdr2-2-specific CD8(+) T cells in the pathogenesis of PCD.
Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, United States.
Erica L
EL
Carpenter
Barbara A
BA
Vance
Rachel S
RS
Klein
Alfredo
A
Voloschin
Josep
J
Dalmau
Robert H
RH
Vonderheide
D000328:Adult; D000368:Aged; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D015789:HLA-A2 Antigen / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation; D052118:Lysosomal-Associated Membrane Protein 1 / Q000032:analysis; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000276:immunology* / Q000378:metabolism; D020362:Paraneoplastic Cerebellar Degeneration / Q000209:etiology / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
C095184:CDR2 protein, human; D015789:HLA-A2 Antigen; D052118:Lysosomal-Associated Membrane Protein 1; D009419:Nerve Tissue Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma