A new 16S rDNA-RFLP method for the discrimination of the accepted species of Arcobacter.
The genus Arcobacter that includes some species of clinical interest has been recently enlarged with the addition of several new species. The available molecular techniques for the characterization of Arcobacter spp. are unable to identify all the species included in this genus. We have developed a 16S rDNA-RFLP method able to separate the currently accepted 6 species of Arcobacter, including the 2 hybridization groups of Arcobacter cryaerophilus. The method based on the use of a pair of primers and of the endonuclease MseI has been validated using 12 reference strains (including the type strains) and 75 fresh isolates. All isolates tested produced species-specific RFLP patterns. This easy-to-perform method allows a fast and reliable recognition of the members of this complex genus.
Figueras
Maria José
MJ
Unitat de Microbiologia, Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Sant Llorenç 21, Reus, Spain. mariajose.figueras@urv.cat
Collado
Luis
L
Guarro
Josep
J
D000818:Animals; D020640:Arcobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D002417:Cattle; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D011817:Rabbits; D013045:Species Specificity
D017931:DNA Primers; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C055927:endodeoxyribonuclease MseI; D015252:Deoxyribonucleases, Type II Site-Specific