[Surface enhanced Raman spectroscopic study on the gold-labeled protein self-assembled surface].
The human IgG molecules were labeled with 13 nm gold nanoparticles and the complex of the gold-labeled human IgG molecules was immobilized on a silicon surface modified by 3-aminopropyltriethoxysilane and glutaraldehyde. The method increases not only the tightness but also the surface coverage for immobilization of the complex and retains protein configuration well on the silicon surface. The self-assembled complex surface was observed by AFM. The complex aggregated on the silicon surface and the "island" monolayer of the complex was obtained. Meanwhile the SERS spectrum of the complex self-assembled "island" monolayer on silicon surface was presented. In the present paper, the gold labeled human IgG molecules were self-assembled on the silicon surface, SERS spectra of protein were obtained and as SERS active substrates were provided for the study of the protein molecules.
Key Laboratory of Excited State Processes, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China.
Ke-Fu
KF
Chao
You-Lin
YL
Zhang
Xiang-Gui
XG
Kong
Li-Yun
LY
Feng
Bing
B
Li
Qing-Hui
QH
Zeng
Kai
K
Song
Ya-Juan
YJ
Sun
D006046:Gold / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
D007074:Immunoglobulin G; D006046:Gold