Evaluation of the biocompatibility of a new vascular prosthesis coating by detection of prosthesis-specific antibodies.
In recent experimental studies, we could demonstrate the occurrence of antibodies against the prosthesis matrix and coating following implantation of polyester-based vascular grafts. Therefore, this study aimed at evaluating the biocompatibility of a new absorbable polymer coating by detection of antibodies against the coating and the polyester matrix. Two polyester vascular prostheses coated either with the polymer (PP-prosthesis) or with gelatine (PG-prosthesis) were functionally implanted into sheep (n = 22 per group). Blood was drawn on days 1 (pre-OP) and 7, 14, 28, 56, 84, 140 (post-OP). Homogenates from both prostheses (PP-target or PG-target) or from an uncoated prosthesis (P-target) were used as assay targets in a particle-based immunoassay. The antibody binding against the P-target was significantly higher in the PP-group than in the PG-group on days 7-56, but not on days 84 and 140. Within both groups, no significant differences but a significant correlation between the binding against the P-target and the coated target was found. Therefore, the absorbable polymer did not induce a specific humoral immune response. In conclusion, the overall immunogenicity of the polymer-coated graft was comparable to the gelatine-coated graft. The detection of prosthesis-specific antibodies seems to be useful for in vivo biocompatibility testing.
Walschus
Uwe
U
Research Group of Predictive Diagnostics, Department of Medical Biochemistry and Molecular Biology, Ernst Moritz Arndt University Greifswald, Greifswalder Str. 11c, 17495 Karlsburg, Germany.
Goldmann
Helmut
H
Ueberrueck
Torsten
T
Hoene
Andreas
A
Wilhelm
Lutz
L
Schlosser
Michael
M
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation / Q000379:methods*; D020099:Coated Materials, Biocompatible; D005780:Gelatin / Q000737:chemistry; D007111:Immunity, Cellular; D007118:Immunoassay / Q000379:methods; D008863:Microspheres; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D019919:Prosthesis Implantation; D012756:Sheep; D013997:Time Factors
D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D011091:Polyesters; D011108:Polymers; D005780:Gelatin