Compressive force stimulates the expression of osteogenesis-related transcription factors in ROS 17/2.8 cells.
OBJECTIVE
To determine how compressive force affects the expression of osteogenesis-related transcription factors in osteoblasts.


METHODS
Cells of ROS 17/2.8, a typical osteoblastic cell line, were cultured with or without continuous compressive force (0.5-2.0 g/cm(2)). Expression of mRNA encoding the osteogenesis-related transcription factors Runx2, Osterix, Msx2, Dlx5 and AJ18 was measured using real-time polymerase chain reaction. Protein expression of these transcription factors was determined by Western blotting.


RESULTS
A compressive force of 1.0 g/cm(2) significantly increased mRNA and protein expression of Runx2, Osterix, Msx2 and Dlx5, which are critical for osteoblast differentiation. In contrast, mRNA and protein expression of AJ18, which downregulates osteoblast differentiation, were decreased with 1.0 g/cm(2) of compressive force.


CONCLUSIONS
A compressive force of 1.0 g/cm(2), which was considered optimal for bone formation under the present experimental conditions, stimulates osteoblastic differentiation via the modulation of osteogenesis-related transcription factors.
Yanagisawa
Momoko
M
Department of Orthodontics, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo, Japan.
Suzuki
Naoto
N
Mitsui
Narihiro
N
Koyama
Yuki
Y
Otsuka
Kichibee
K
Shimizu
Noriyoshi
N
D015153:Blotting, Western / Q000379:methods; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis / Q000235:genetics; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D010006:Osteoblasts / Q000378:metabolism*; D010012:Osteogenesis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000076182:Sp7 Transcription Factor; D013314:Stress, Mechanical; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics*
D050659:Core Binding Factor Alpha 1 Subunit; C100596:DLX5 protein, human; D018398:Homeodomain Proteins; C084119:MSX2 protein; D012333:RNA, Messenger; D000076182:Sp7 Transcription Factor; C478163:SP7 protein, human; D014157:Transcription Factors