In vivo effects of chronic contamination with 137 cesium on testicular and adrenal steroidogenesis.
More than 20 years after Chernobyl nuclear power plant explosion, radionuclides are still mainly bound to the organic soil layers. The radiation exposure is dominated by the external exposure to gamma-radiation following the decay of (137)Cs and by soil-to-plant-to-human transfer of (137)Cs into the food chain. Because of this persistence of contamination with (137)Cs, questions regarding public health for people living in contaminated areas were raised. We investigated the biological effects of chronic exposure to (137)Cs on testicular and adrenal steroidogenesis metabolisms in rat. Animals were exposed to radionuclide in their drinking water for 9 months at a dose of 6,500 Bq/l (610 Bq/kg/day). Cesium contamination decreases the level of circulating 17beta-estradiol, and increases corticosterone level. In testis, several nuclear receptors messenger expression is disrupted; levels of mRNA encoding Liver X receptor alpha (LXRalpha) and LXRbeta are increased, whereas farnesoid X receptor mRNA presents a lower level. Adrenal metabolism presents a paradoxical decrease in cyp11a1 gene expression. In conclusion, our results show for the first time molecular and hormonal modifications in testicular and adrenal steroidogenic metabolism, induced by chronic contamination with low doses of (137)Cs.
Grignard
Elise
E
Radiological Protection and Human Health Division, Radiobiology and Epidemiology Department, Laboratory of Experimental Toxicology, Institute for Radiological Protection and Nuclear Safety, Fontenay-aux-Roses Cedex, France. elise.grignard@irsn.fr
Guéguen
Yann
Y
Grison
Stéphane
S
Lobaccaro
Jean-Marc A
JM
Gourmelon
Patrick
P
Souidi
Maâmar
M
D000311:Adrenal Glands / Q000378:metabolism* / Q000528:radiation effects*; D000818:Animals; D002588:Cesium Radioisotopes / Q000633:toxicity*; D017931:DNA Primers; D006304:Health Status; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013256:Steroids / Q000096:biosynthesis* / Q000097:blood; D013737:Testis / Q000378:metabolism* / Q000528:radiation effects*
D002588:Cesium Radioisotopes; D017931:DNA Primers; D012333:RNA, Messenger; D013256:Steroids