ATP synthesis by decarboxylation phosphorylation.
Adenosine triphosphate (ATP) is used as a general energy source by all living cells. The free energy released by hydrolyzing its terminal phosphoric acid anhydride bond to yield ADP and phosphate is utilized to drive various energy-consuming reactions. The ubiquitous F(1)F(0) ATP synthase produces the majority of ATP by converting the energy stored in a transmembrane electrochemical gradient of H(+) or Na(+) into mechanical rotation. While the mechanism of ATP synthesis by the ATP synthase itself is universal, diverse biological reactions are used by different cells to energize the membrane. Oxidative phosphorylation in mitochondria or aerobic bacteria and photophosphorylation in plants are well-known processes. Less familiar are fermentation reactions performed by anaerobic bacteria, wherein the free energy of the decarboxylation of certain metabolites is converted into an electrochemical gradient of Na(+) ions across the membrane (decarboxylation phosphorylation). This chapter will focus on the latter mechanism, presenting an updated survey on the Na(+)-translocating decarboxylases from various organisms. In the second part, we provide a detailed description of the F(1)F(0) ATP synthases with special emphasis on the Na(+)-translocating variant of these enzymes.
Dimroth
Peter
P
Institute of Microbiology, ETH Zürich, Wolfgang-Pauli-Strasse 10, 8093, Zürich, Switzerland. dimroth@micro.biol.ethz.ch
von Ballmoos
Christoph
C
D000255:Adenosine Triphosphate / Q000737:chemistry* / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism; D018407:Bacterial Physiological Phenomena; D001692:Biological Transport; D002951:Citrates / Q000737:chemistry; D003653:Decarboxylation; D004563:Electrochemistry / Q000379:methods; D005285:Fermentation; D007477:Ions; D008928:Mitochondria / Q000378:metabolism; D008954:Models, Biological; D008958:Models, Molecular; D010085:Oxidative Phosphorylation; D010766:Phosphorylation; D012964:Sodium / Q000737:chemistry
D002951:Citrates; D007477:Ions; D000255:Adenosine Triphosphate; D012964:Sodium