A patient with hyperglycaemia and normal HbA Ic due to impaired glycation.
A diabetic Caucasian woman presented with discrepantly low HbA Ic values compared with her glycaemia. High-performance liquid chromatography (HPLC) analysis disclosed 80% HbA and 20% HbI Philadelphia (I6alpha2 lys --> glut). The calculated glycosylation gap from the fructosamine level was 1.2%. The haemoglobin alpha/beta glycation ratios, as measured by electron spray ionisation mass spectroscopy (ESI-MS), for the patient and her three children also carrying the mutation were decreased by values of 0.56 and 0.51, 0.50 and 0.49, respectively (reference value 0.66).
Department of Internal Medicine, Deventer Hospital, Deventer, the Netherlands. m.j.m.diekman@dz.nl
M J M
MJ
Diekman
H J M
HJ
Salden
J H
JH
DeVries
D000368:Aged; D002851:Chromatography, High Pressure Liquid; D005260:Female; D019270:Fructosamine; D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced; D006031:Glycosylation; D006801:Humans; D006943:Hyperglycemia / Q000503:physiopathology*; D013058:Mass Spectrometry
D019270:Fructosamine; D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced