Association of vitamin D receptor gene Taq I polymorphism with recurrent urolithiasis in children.
OBJECTIVE
Urolithiasis has a strong familial component. However, to date, no specific genetic abnormality has been identified. It has been reported that allelic variation in the vitamin D receptor (VDR) gene may affect calcium absorption and excretion. Urolithiasis is a multifactorial disease in which both genetic and environmental factors have an effect on onset and severity of disease. In the present study, the role of Taq I polymorphism of vitamin D receptor gene in urolithiasis was studied.


METHODS
Eighty children with calcium stone disease (40 with single episode of stone disease and 40 with recurrence) and 40 controls were enrolled. Polymorphic sites were amplified by polymerase chain reaction, digested with Taq I restriction enzymes and analyzed by gel electrophoresis. Allelic or genotypic frequencies were calculated and associations between them and the presence of hypercalciuria, family history and stone recurrence were evaluated.


RESULTS
Incidence of Taq I tt genotypes was significantly higher in patients with recurrent calcium-stone disease compared to the controls. In addition, the frequency of the 't' allele was higher in recurrent calcium-stone formers. Taq I t allele was found to be associated with increased risk of recurrence. No association between Taq I polymorphism and a positive family history was found in the present study. The frequency of hypercalciuria was higher in patients with the 'tt' genotype.


CONCLUSIONS
Taq I t allele of the VDR gene may be a risk factor for severe urolithiasis and recurrent stone disease.
Uludag University, Faculty of Medicine, Department of Urology, Bursa, Turkey.
Serkan
S
Seyhan
Ismet
I
Yavascaoglu
Hakan
H
Kilicarslan
Hasan S
HS
Dogan
Yakup
Y
Kordan
D000293:Adolescent; D002648:Child; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics*; D018167:Receptors, Calcitriol / Q000235:genetics*; D012008:Recurrence; D052878:Urolithiasis / Q000235:genetics*
D018167:Receptors, Calcitriol; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases