Jonquetella anthropi gen. nov., sp. nov., the first member of the candidate phylum 'Synergistetes' isolated from man.
Six clinical isolates of a hitherto unknown, strictly anaerobic, Gram-negative rod showing fastidious growth were subjected to a polyphasic taxonomic study, including phenotypic, genomic and phylogenetic feature analyses. 16S rRNA gene sequenced-based phylogeny revealed that the novel strains represent a homogeneous group distant from any recognized species in the candidate phylum 'Synergistetes'. The novel isolates were most closely related to species of the genus Dethiosulfovibrio, with 88.2-88.7 % 16S rRNA gene sequence similarity. Large-scale chromosome structure and DNA G+C content also differentiated the novel strains from members of the genus Dethiosulfovibrio. The novel strains were asaccharolytic. Major metabolic end products in trypticase/glucose/yeast extract broth were acetic, lactic, succinic and isovaleric acids and the major cellular fatty acids iso-C(15 : 0) and C(16 : 0). Based on the data presented here, a new genus, Jonquetella gen. nov., is proposed with one novel species, Jonquetella anthropi sp. nov. J. anthropi is the first characterized species of the candidate phylum 'Synergistetes' that includes human isolates. The G+C content of the DNA of the type strain of J. anthropi ADV 126(T) (=AIP 136.05(T)=CIP 109408(T)=CCUG 53819(T)) is 59.4 mol%.
Université Montpellier 1, EA 3755, Faculté de Pharmacie, Laboratoire de Bactériologie-Virologie, 15, Avenue Charles Flahault, BP 14491, 34093 Montpellier Cedex 5, France.
Estelle
E
Jumas-Bilak
Institut Pasteur, Centre National de Référence des Bactéries Anaérobies et du Botulisme, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
Jean-Philippe
JP
Carlier
Centre Hospitalier Universitaire de Montpellier, Hôpital Arnaud de Villeneuve, Laboratoire de Bactériologie, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
Hélène
H
Jean-Pierre
R. M. Alden Research Laboratory, Santa Monica Blvd, Santa Monica, CA 90404, USA.
Diane
D
Citron
National Microbiology Laboratory - Public Health Agency of Canada, 1015 Arlington St., Suite H5040, Winnipeg, MB R3E 3R2, Canada.
Kathryn
K
Bernard
Centre Hospitalier Universitaire de Montpellier, Hôpital Arnaud de Villeneuve, Laboratoire de Bactériologie, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
Audrey
A
Damay
Centre Hospitalier Universitaire de Montpellier, Hôpital Arnaud de Villeneuve, Laboratoire de Bactériologie, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
Bernard
B
Gay
Université Montpellier 1, EA 3755, Faculté de Pharmacie, Laboratoire de Bactériologie-Virologie, 15, Avenue Charles Flahault, BP 14491, 34093 Montpellier Cedex 5, France.
Corinne
C
Teyssier
Centre Hospitalier Universitaire de Montpellier, Hôpital Arnaud de Villeneuve, Laboratoire de Bactériologie, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
Josiane
J
Campos
Centre Hospitalier Universitaire de Montpellier, Hôpital Arnaud de Villeneuve, Laboratoire de Bactériologie, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.|Université Montpellier 1, EA 3755, Faculté de Pharmacie, Laboratoire de Bactériologie-Virologie, 15, Avenue Charles Flahault, BP 14491, 34093 Montpellier Cedex 5, France.
Hélène
H
Marchandin
D019342:Acetic Acid / Q000378:metabolism; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000145:classification* / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D045782:Hemiterpenes; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010421:Pentanoic Acids / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D019802:Succinic Acid / Q000378:metabolism
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D045782:Hemiterpenes; D010421:Pentanoic Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C008216:isovaleric acid; D019344:Lactic Acid; D019802:Succinic Acid; D019342:Acetic Acid