A new monoterpene glycoside from the roots of Paeonia lactiflora increases the differentiation of osteoblastic MC3T3-E1 cells.
A new monoterpene glycoside, 6'-O-beta-D-glucopyranosylalbiflorin (1), and four known compounds; albiflorin (2), 6'-O-benzoylalbiflorin (3), paeoniflorin (4) and benzoyl paeoniflorin (5), were isolated from the methanolic extract of the roots of Paeonia lactiflora Pall.. Their chemical structures were completely elucidated using a combination of 2D NMR techniques (COSY, HMQC and HMBC) and HRESI-MS analyses. To investigate the bioactivities of these compounds, their effects on the differentiation of osteoblastic MC3T3-E1 cells were tested. Compound 1 (0.01-10 microM) significantly increased the alkaline phosphatase activity and nodules mineralization of MC3T3-E1 cells compared to those of the control (P<0.05). These results suggest that newly isolated compound 1 has a direct stimulatory effect on bone formation in vitro and may contribute to the prevention for osteoporosis.
Institute of Natural Products Chemistry, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Nghiado, Caugiay Hanoi, Vietnam.
Pham Hai
PH
Yen
Phan Van
PV
Kiem
Nguyen Xuan
NX
Nhiem
Nguyen Huu
NH
Tung
Tran Hong
TH
Quang
Chau Van
CV
Minh
Jung Woo
JW
Kim
Eun Mi
EM
Choi
Young Ho
YH
Kim
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001565:Benzoates / Q000494:pharmacology; D050071:Bone Density Conservation Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000494:pharmacology; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure; D039821:Monoterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects* / Q000201:enzymology; D029596:Paeonia / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots; D021241:Spectrometry, Mass, Electrospray Ionization; D014744:Vietnam
C468433:6'-O-benzoylalbiflorin; D001565:Benzoates; D050071:Bone Density Conservation Agents; D001952:Bridged-Ring Compounds; D005960:Glucosides; D006027:Glycosides; D039821:Monoterpenes; D010936:Plant Extracts; C015423:peoniflorin; C047591:benzoylpaeoniflorin; C014959:albiflorin; D000469:Alkaline Phosphatase