Genetic analysis of phosphoprotein and matrix protein of rabies viruses isolated in Brazil.
To investigate the genetic characteristics of phosphoprotein (P) and matrix protein (M) genes of variable rabies virus (RV) prevalent in Brazil, the authors genetically characterized the P and M genes from 30 Brazilian RV field isolates. Phylogenetic analysis based on the P and M genes revealed the presence of six RV variants that consisted primarily of three insectivorous bats, the vampire bat, dog and fox in Brazil. Specific amino acid substitutions corresponding to these phylogenetic lineages were observed, with Asp(42) and Glu(62) in the P protein found to be characteristic of Brazilian chiroptera- and carnivora-related RVs, respectively. Amino acid sequence motifs predicted to associate with a viral function in the P and M proteins were conserved among Brazilian RV variants.
Nihon University Veterinary Research Center, 1866 kameino, Fukisawa, Kanagawa 252-8510, Japan.
Yuki
Y
Kobayashi
Hiromi
H
Okuda
Kana
K
Nakamura
Go
G
Sato
Takuya
T
Itou
Adolorata A B
AA
Carvalho
Marlon V
MV
Silva
Carla S
CS
Mota
Fumio H
FH
Ito
Takeo
T
Sakai
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001938:Brazil; D014644:Genetic Variation; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics*; D010802:Phylogeny; D011820:Rabies virus / Q000235:genetics* / Q000302:isolation & purification; D014763:Viral Matrix Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
D018832:Molecular Chaperones; C103961:P phosphoprotein, Rabies virus; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; D015678:Viral Structural Proteins