Complete 1H and 13C NMR assignments of three new polyhydroxylated sterols from the South China Sea gorgonian Subergorgia suberosa.
Three new polyhydroxylated sterols, 3beta,6alpha,11,20beta,24-pentahydroxy- 9,11-seco-5alpha-24-ethylcholest-7,28-diene-9-one (1), 3-(1',2'-ethandiol)-24- methylcholest-8(9),22E-diene-3beta,5alpha,6alpha,7alpha,11alpha-pentaol (2), 24-methylcholest-7,22 E-diene-3beta,5alpha,6beta,25-tetraol (3) together with five known sterols, were isolated from the EtOH/CH2Cl2 extract of the South China Sea gorgonian Subergorgia suberosa. The complete assignments of the 1H and 13C NMR chemical shifts for these new compounds were achieved by means of 1D and 2D NMR techniques, including HSQC, HMBC, 1H--1H COSY, and NOESY spectra.
Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, The Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301 Guangdong, China. shuhuaqi2001@yahoo.com
Shu-Hua
SH
Qi
Si
S
Zhang
Yi-Fei
YF
Wang
Ming-Yi
MY
Li
D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D002247:Carbon Isotopes; D002681:China; D009682:Magnetic Resonance Spectroscopy / Q000379:methods* / Q000592:standards*; D008968:Molecular Conformation; D011522:Protons; D012015:Reference Standards; D012680:Sensitivity and Specificity; D012855:Sitosterols / Q000737:chemistry* / Q000302:isolation & purification; D013045:Species Specificity; D013237:Stereoisomerism; D013261:Sterols / Q000737:chemistry* / Q000302:isolation & purification
C526249:24-methylcholest-7,22 E-diene-3beta,5alpha,6beta,25-tetraol; C526248:3-(1',2'-ethandiol)-24-methylcholest-8(9),22E-diene-3beta,5alpha,6alpha,7alpha,11alpha-pentaol; C526247:3beta,6alpha,11,20beta,24-pentahydroxy- 9,11-seco-5alpha-24-ethylcholest-7,28-diene-9-one; D002247:Carbon Isotopes; D011522:Protons; D012855:Sitosterols; D013261:Sterols