[Study of Raman spectra of single carcinoma of nasopharynx cell].
The Raman spectra from carcinoma of nasopharynx cell lines (CNE2) and normal airway epithelial cell lines (HBE) were investigated using a laser tweezers Raman spectroscopy (LTRS). The Raman scattering measurements were obtained from three different places in every single cell. Visual inspection of the spectra shows that the differences observed in spectra of the cancer cells and normal cells are obvious. The peak ratio I1 304/I1 336 is 1.05 for the normal cell and 1.22 for the cancer cell. Using a combination of principal component analysis (PCA) and discriminant function analysis (DFA), the authors are able to predict cancer cells, and normal cells and the DFA is better for single Raman spectrum. The sampling locations did not seriously affect the result of PCA and DFA. PCA and DFA also show that the uniformity of normal cells is better than that of cancer cells. The results indicate that the Raman spectra may offer the experimental basis for colorectal cancer diagnosis.
Yao
Hui-Lu
HL
Laboratory for Biophysics, Guangxi Academy of Sciences, Nanning 530003, China. yaohl@163.net
Zhu
Miao
M
Wang
Gui-Wen
GW
Peng
Li-Xin
LX
He
Bi-Juan
BJ
He
Min
M
Li
Yong-Qing
YQ
D002277:Carcinoma / Q000737:chemistry* / Q000175:diagnosis; D045744:Cell Line, Tumor; D002477:Cells / Q000737:chemistry / Q000166:cytology*; D019937:Diagnostic Techniques and Procedures; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000737:chemistry* / Q000175:diagnosis; D013059:Spectrum Analysis, Raman / Q000379:methods*