A highly selective fluorescent probe for thiol bioimaging.
A new fluorescent turn-on probe (3) for the selective sensing and bioimaging of thiols is reported. In aqueous buffer solutions at physiological pH, thiols cleave the 2,4-dinitrobenzenesulfonyl group to release the red-emissive donor-acceptor fluorophore (4). The probe displays excellent immunity to interference from nitrogen and oxygen nucleophiles and the imaging of thiols in living cells is demonstrated.
Bouffard
Jean
J
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Kim
Youngmi
Y
Swager
Timothy M
TM
Weissleder
Ralph
R
Hilderbrand
Scott A
SA
D003124:Colorimetry / Q000379:methods; D003952:Diagnostic Imaging / Q000379:methods*; D005456:Fluorescent Dyes; D015394:Molecular Structure; D009578:Nitrobenzenes / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000032:analysis*
D005456:Fluorescent Dyes; D009578:Nitrobenzenes; D013438:Sulfhydryl Compounds; D009584:Nitrogen; D010100:Oxygen