Estrogen-like activities in Vitex species from China determined by a cell based proliferation assay.
Ethanolic extracts of four Chinese medicinally used Vitex species were selected and tested for their estrogen-like activities, using an ERalpha-positive MCF-7 cell based proliferation assay (E-screen assay) and cell cycle analysis (flow cytometry). Vitex negundo displayed the highest estrogenic-like activity, and could be useful in hormone replacement therapy (HRT).
Hu
Y
Y
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai, China.
Zhang
Q Y
QY
Hou
T T
TT
Xin
H L
HL
Zheng
H C
HC
Rahman
K
K
Qin
L P
LP
D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002606:Charcoal; D002681:China; D003911:Dextrans; D004968:Estrogens, Non-Steroidal; D005434:Flow Cytometry; D006801:Humans; D007202:Indicators and Reagents; D010936:Plant Extracts / Q000494:pharmacology; D044967:Serum / Q000737:chemistry; D020950:Vitex / Q000737:chemistry*
D003911:Dextrans; D004968:Estrogens, Non-Steroidal; D007202:Indicators and Reagents; D010936:Plant Extracts; D002606:Charcoal