Insertional mutagenesis strategies in zebrafish.
We review here some recent developments in the field of insertional mutagenesis in zebrafish. We highlight the advantages and limitations of the rich body of retroviral methodologies, and we focus on the mechanisms and concepts of new transposon-based mutagenesis approaches under development, including prospects for conditional 'gene trapping' and 'gene breaking' approaches.
Sivasubbu
Sridhar
S
Institute of Genomics and Integrative Biology, Council for Scientific and Industrial Research, Mall Road, Delhi 110007, India.
Balciunas
Darius
D
Amsterdam
Adam
A
Ekker
Stephen C
SC
D000818:Animals; D004251:DNA Transposable Elements; D053595:Embryonic Stem Cells / Q000378:metabolism; D016254:Mutagenesis, Insertional / Q000379:methods*; D012190:Retroviridae; D015027:Zebrafish / Q000235:genetics* / Q000821:virology
D004251:DNA Transposable Elements