Tumor specific ultrasound enhanced gene transfer in vivo with novel liposomal bubbles.
Bubble liposomes (liposomes which entrap an ultrasound imaging gas) may constitute a unique system for delivering various molecules efficiently into mammalian cells in vitro. In this study, Bubble liposomes were compared with cationic lipid (CL)-DNA complexes as potential gene delivery carriers into tumor in vivo. The delivery of genes by Bubble liposomes depended on the intensity of the applied ultrasound. Transfection efficiency plateaued at 0.7 W/cm(2) ultrasound intensity. Bubble liposomes efficiently transferred genes into cultured cells even when the cells were exposed to ultrasound for only 1 s. In addition, Bubble liposomes could introduce the luciferase gene more effectively than CL-DNA complexes into mouse ascites tumor cells and solid tumor tissue. We conclude that the combination of Bubble liposomes and ultrasound is a minimally-invasive and tumor specific gene transfer method in vivo.
Department of Biopharmaceutics, School of Pharmaceutical Sciences, Teikyo University, 1091-1 Suwarashi, Sagamiko, Sagamihara, Kanagawa 229-0195, Japan.
Ryo
R
Suzuki
Tomoko
T
Takizawa
Yoichi
Y
Negishi
Naoki
N
Utoguchi
Kaori
K
Sawamura
Kumiko
K
Tanaka
Eisuke
E
Namai
Yusuke
Y
Oda
Yasuhiro
Y
Matsumura
Kazuo
K
Maruyama
D000818:Animals; D019556:COS Cells; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D004247:DNA / Q000008:administration & dosage / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects; D005466:Fluorocarbons / Q000008:administration & dosage / Q000378:metabolism*; D018390:Gene Targeting / Q000379:methods; D015316:Genetic Therapy / Q000379:methods*; D006461:Hemolysis / Q000187:drug effects; D008081:Liposomes / Q000008:administration & dosage / Q000378:metabolism*; D008297:Male; D051379:Mice; D012510:Sarcoma 180 / Q000378:metabolism*; D014162:Transfection / Q000379:methods*; D014465:Ultrasonics
D005466:Fluorocarbons; D008081:Liposomes; D004247:DNA; C042852:perflutren