Atg7-dependent autophagy promotes neuronal health, stress tolerance, and longevity but is dispensable for metamorphosis in Drosophila.
Autophagy, a cellular process of cytoplasmic degradation and recycling, is induced in Drosophila larval tissues during metamorphosis, potentially contributing to their destruction or reorganization. Unexpectedly, we find that flies lacking the core autophagy regulator Atg7 are viable, despite severe defects in autophagy. Although metamorphic cell death is perturbed in Atg7 mutants, the larval-adult midgut transition proceeds normally, with extended pupal development compensating for reduced autophagy. Atg7-/- adults are short-lived, hypersensitive to nutrient and oxidative stress, and accumulate ubiquitin-positive aggregates in degenerating neurons. Thus, normal levels of autophagy are crucial for stress survival and continuous cellular renewal, but not metamorphosis.
Juhász
Gábor
G
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Erdi
Balázs
B
Sass
Miklós
M
Neufeld
Thomas P
TP
D000818:Animals; D001343:Autophagy / Q000235:genetics* / Q000502:physiology*; D000071193:Autophagy-Related Protein 7; D004064:Digestive System / Q000166:cytology / Q000254:growth & development; D004330:Drosophila / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics* / Q000502:physiology*; D017353:Gene Deletion; D017344:Genes, Insect; D007814:Larva / Q000166:cytology / Q000254:growth & development; D008136:Longevity / Q000235:genetics / Q000502:physiology; D008297:Male; D008675:Metamorphosis, Biological; D009154:Mutation; D009410:Nerve Degeneration / Q000235:genetics; D009474:Neurons / Q000166:cytology / Q000502:physiology; D018384:Oxidative Stress; D013217:Starvation; D044764:Ubiquitin-Activating Enzymes / Q000235:genetics* / Q000502:physiology*
C530193:Atg7 protein, Drosophila; D029721:Drosophila Proteins; D000071193:Autophagy-Related Protein 7; D044764:Ubiquitin-Activating Enzymes