Evaluation of quantitative methods for the determination of polyphenols in algal extracts.
Marine brown algae such as Ascophyllum nodosum and Fucus vesiculosus accumulate polyphenols composed of phloroglucinol units. These compounds are of ecological importance and, due to their antioxidative activity, of pharmacological value as well. In this study four methods for the quantitative determination of phlorotannins are compared: spectrophotometric determinations using Folin-Ciocalteu's phenol reagent or 2,4-dimethoxybenzaldehyde (DMBA), quantitative (1)H NMR spectroscopy (qHNMR), and gravimetrical measurements. On the basis of the relative standard deviation and the F-test, the determination using Folin-Ciocalteu's phenol reagent and qHNMR proved to be the most reliable and precise methods.
Parys
Sabine
S
Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, D-53115, Bonn, Germany.
Rosenbaum
Anne
A
Kehraus
Stefan
S
Reher
Gerrit
G
Glombitza
Karl-Werner
KW
König
Gabriele M
GM
D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D044646:Ascophyllum / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005602:France; D044645:Fucus / Q000737:chemistry*; D008386:Marine Biology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010636:Phenols / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D059808:Polyphenols; D013634:Tannins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013634:Tannins