[Extraction of mitochondrial DNA of Tilletia indica Mitra and application of the ATP6 gene on fungal phylogenetic analysis].
Tilletia indica Mitra, an important pest around the world which is the causative agent of Karnal Bunt of wheat, was very close to Tilletia walkeri phylogenetically and morphologically. 69.6 ng mitochondrial DNA (mtDNA) of Tilletia indica obtained from 6.4 mg total DNA by the method of CsCl/bisbenzimide density gradient ultracentrifugation can be used for electrophoresis, cloning, enzyme restriction analysis and PCR amplification. The ATP6 gene sequence was cloned from the fragment of mtDNA and sequenced for analysis of phylogenetic tree with the other related sequences in GenBank by the software PAUP to reveal the phylogenetic relationships within the Tilletia species.
Department of Food and Bioengineering, Jiangsu University, Zhenjiang 212013, China. zhouyeqin@163.com
Ye-Qin
YQ
Zhou
Jian-Ping
JP
Yi
Guo-Liang
GL
Zhou
Ying
Y
Dong
D001487:Basidiomycota / Q000145:classification* / Q000235:genetics*; D004271:DNA, Fungal / Q000302:isolation & purification*; D004272:DNA, Mitochondrial / Q000302:isolation & purification*; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000382:microbiology
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D025261:Mitochondrial Proton-Translocating ATPases