Statistical optimization of medium components for the production of biosurfactant by Bacillus licheniformis K51.
The nutritional medium requirement for biosurfactant production by Bacillus licheniformis K51 was optimized. The important medium components, identified by the initial screening method of Plackett-Burman, were H3PO4, CaCl2, H3BO3, and Na-EDTA. Box-Behnken response surface methodology was applied to further optimize biosurfactant production. The optimal concentrations for higher production of biosurfactants were (g/l): glucose, 1.1; NaNO3, 4.4; MgSO4 x 7H2O, 0.8; KCl, 0.4; CaCl2, 0.27; H3PO4, 1.0 ml/l; and trace elements (mg/l): H3BO3, 0.25; CuSO4, 0.6; MnSO4, 2.2; Na2MoO4, 0.5; ZnSO4, 6.0; FeSO4, 8.0; CoCl2, 1.0; and Na-EDTA, 30.0. Using this statistical optimization method, the relative biosurfactant yield as critical micelle dilution (CMD) was increased from 10x to 105x, which is ten times higher than the non-optimized rich medium.
Department of Microbiology and Biotechnology Centre, Maharaja Sayajirao University of Baroda, Vadodara 390002, Gujarat, India.
Sanket
S
Joshi
Sanjay
S
Yadav
Anuradha
A
Nerurkar
Anjana J
AJ
Desai
D001407:Bacillus / Q000378:metabolism*; D003470:Culture Media; D008823:Micelles; D013501:Surface-Active Agents / Q000378:metabolism*
D003470:Culture Media; D008823:Micelles; D013501:Surface-Active Agents