[Development and forensic application of a pentaplex X-STR loci typing system].
A fluorescent multiplex PCR that simultaneously amplifies five X-chromosomal short tandem repeat (X-STR) loci (DXS6803, DXS981, DXS6809, DXS6789 and DXS7132)was set up to study their polymorphic nature and to determine its use in kinship tests for forensic cases. PCR products were analyzed using capillary electrophoresis and ABI prism 3100 Genetic Analyzer with GeneMapper ID 3.1 Analysis Software. The pentaplex system gave satisfactory results as its sensitivity, reproducibility and unambiguous genotyping. About 20 ng DNA was routinely used, although 0.25 ng DNA was sufficient for allele typing. The results demonstrate that the multiplex system of the five X-STR loci provides a fast technology for forensic identification and paternity testing. The X-STR pentaplex system can complement the analysis of AS-STR and Y-STR efficiently, especially in complex cases of kinship testing.
Liu
Qiu-Ling
QL
Faculty of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510089, China. liuql@mail.sysu.edu.cn
Lv
De-Jian
DJ
Sun
Hong-Yu
HY
Lu
Hui-Ling
HL
Wu
Xiang-Lin
XL
Wu
Xin-Yao
XY
D000483:Alleles; D041321:Chromosomes, Human, X / Q000235:genetics*; D005260:Female; D005453:Fluorescence; D053803:Forensic Genetics / Q000379:methods*; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D035781:Siblings