Increased expression of catalase in human hepatoma cells by the soy isoflavone, daidzein.
The reduced incidence of cancer that has been observed in Asian population traditionally consuming soy-based food has been linked to the antioxidant potential of soy isoflavones, in particular daidzein and genistein. The present study was undertaken in order to test the antioxidative potential of daidzein and to examine the effect of daidzein treatment on the expression of the antioxidant enzyme catalase in the human hepatoma cell lines Huh-7 and HepG2. Daidzein itself did not display radical scavenging activity but it significantly increased the activity of the antioxidant enzyme catalase. Huh-7 cells were much more susceptible to daidzein cytotoxicity than HepG2 cells and showed much lower basal activity in luciferase reporter gene assays with the 3.2 kb fragment of the human catalase promoter. However, treatment with daidzein at a non-toxic concentration resulted in a similar induction of promoter activity in both cell lines. Reporter gene studies with different promoter constructs in HepG2 cells restrict the potential localization of the main regulatory elements for basal and inducible activity of the catalase promoter to a region approximately 120 bp to 300 bp upstream of the start codon of the catalase gene. From our results, we conclude that in human hepatoma cells daidzein at a non-toxic concentration increases the activity of human catalase and induces the transcription of the catalase gene via interaction with the proximal part of the promoter.
Institute of Toxicology, University of Düsseldorf, Düsseldorf, Germany. kampkoet@uni-duesseldorf.de
Andreas
A
Kampkötter
Christian
C
Wiegand
Claudia
C
Timpel
Elke
E
Röhrdanz
Yvonni
Y
Chovolou
Regine
R
Kahl
Wim
W
Wätjen
D000975:Antioxidants / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002474:Cell-Free System; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D008113:Liver Neoplasms; D011401:Promoter Regions, Genetic; D013025:Glycine max; D014158:Transcription, Genetic
D000975:Antioxidants; D007529:Isoflavones; C004742:daidzein; D002374:Catalase