Bacterial surface display of GFP(uv) on bacillus subtilis spores.
To analyze a cotG-based Bacillus subtilis spore display system directly, GFP(uv) was expressed on the surface of Bacillus subtilis spores. When GFP(uv) was fused to the C-terminal of the cotG structural gene and expressed, the existence of a CotG-GFP(uv) fusion protein on the B. subtilis spore was confirmed by flow cytometry confocal microscopic analysis. When the cotG anchoring motif was deleted, no fluorescence emission was observed under flow cytometry and confocal microscopic analysis from the purified spore, confirming the essential role of CotG as an anchoring motif. This GFP(uv) displaying spore might be used for another signaling application triggered by intracellular or extracellular stimuli.
Department of Chemical Engineering, Dong-A University, Busan 604-714, Korea.
June-Hyung
JH
Kim
Changhyun
C
Roh
Chang-Won
CW
Lee
Dohyun
D
Kyung
Soo-Keun
SK
Choi
Heung-Chae
HC
Jung
Jae-Gu
JG
Pan
Byung-Gee
BG
Kim
D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D013171:Spores, Bacterial / Q000235:genetics
D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; C091352:cotG protein, Bacillus subtilis; D049452:Green Fluorescent Proteins