Near real-time detection of Cryptosporidium parvum oocyst by IgM-functionalized piezoelectric-excited millimeter-sized cantilever biosensor.
Piezoelectric-excited millimeter-sized cantilever (PEMC) biosensors were fabricated and functionalized with immunoglobulin M (IgM) for the detection of Cryptosporidium parvum oocyst in a flow configuration at 1 mL/min. The detection of 100, 1000, and 10,000 oocysts/mL was achieved with a positive sensor response in less than 1 min. Bovine serum albumin (BSA) was used as a blocking agent in each experiment and was shown to eliminate non-specific binding. The sensor's resonance frequency response correlates with C. parvum oocyst concentration logarithmically. The oocyst attachment rate was found to increase by an order of magnitude in increasing concentration from 100 to 10,000 oocysts/mL. The significance of these results is that IgM-functionalized PEMC sensors are highly selective and sensitive to C. parvum oocyst and therefore, have the potential to accurately identify and quantify C. parvum oocyst in drinking water.
Pharmaceutical Development, GlaxoSmithKline, PA 19406, USA.
Gossett A
GA
Campbell
Raj
R
Mutharasan
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003199:Computer Systems; D003458:Cryptosporidium / Q000302:isolation & purification*; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007075:Immunoglobulin M / Q000737:chemistry / Q000276:immunology*; D008904:Miniaturization; D033661:Oocysts / Q000276:immunology*; D014159:Transducers; D014873:Water Pollutants / Q000032:analysis*
D007075:Immunoglobulin M; D014873:Water Pollutants