The repeated immobilization stress increases IL-1beta immunoreactivities in only neuron, but not astrocyte or microglia in hippocampal CA1 region, striatum and paraventricular nucleus.
The effect of repeated immobilization stress (RIS) on the expression of interleukin-1beta (IL-1beta) and types of cells that express IL-1beta in hippocampal CA1 region, striatum and paraventricular nucleus (PVN) were investigated in ICR mice. The RIS was induced daily for 2h for 4 consecutive days. In the immunohistochemical study, RIS increased IL-1beta immunoreactivities (IR) in the hippocampal CA1 region and striatum and PVN. The RIS also increased glial fibrillary acidic protein (GFAP) IR and complement receptor type 3 (OX-42) IR in the hippocampal CA1 regions and striatum but not PVN. In confocal immunofluorescence study, the IL-1beta IR increased by RIS were colocalized with only NeuN, but not GFAP or OX-42 in the hippocampal CA1 region, striatum and PVN. Our results indicate that RIS increases IL-1beta IR on neuron, but not astrocyte or microglia in the hippocampal CA1 region, striatum and PVN, suggesting that the IL-1beta IR on neuron may play an important role during RIS. In addition, GFAP and OX-42 increased by RIS may be involved indirectly in playing another role in the hippocampal CA1 region and striatum during RIS.
Department of Pharmacology, College of Medicine, and Institute of Natural Medicine, Hallym University, 1 Okcheondong, Chuncheon, Gangwon-Do 200-702, South Korea.
Min-Soo
MS
Kwon
Young-Jun
YJ
Seo
Jin-Koo
JK
Lee
Han-Kyu
HK
Lee
Jun-Sub
JS
Jung
Jeong-Eun
JE
Jang
Soo-Hyun
SH
Park
Hong-Won
HW
Suh
D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D039481:CD11b Antigen / Q000032:analysis / Q000378:metabolism; D002908:Chronic Disease; D003342:Corpus Striatum / Q000378:metabolism / Q000503:physiopathology; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism / Q000503:physiopathology; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D017628:Microglia / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism / Q000503:physiopathology; D016548:Prosencephalon / Q000378:metabolism* / Q000503:physiopathology; D012149:Restraint, Physical / Q000523:psychology; D013315:Stress, Psychological / Q000378:metabolism* / Q000503:physiopathology; D015854:Up-Regulation / Q000502:physiology*
D015415:Biomarkers; D039481:CD11b Antigen; D005904:Glial Fibrillary Acidic Protein; C509295:ITGAM protein, human; D053583:Interleukin-1beta