Escherichia coli can produce recombinant chitinase in the soil to control the pathogenesis by Fusarium oxysporum without colonization.
Fusarium wilt of cucumbers was effectively controlled by Escherichia coli expressing an endochitinase gene (chiA), and the rate was as effective (60.0%) as the wildtype strain S. proteamaculans 3095 (55.0%) where the gene was cloned. However, live cells of soil inoculated E. coli host harboring the chiA gene did not proliferate but declined 100-fold from 108 CFU during the first week and showed less than 10 cells after day 14, suggesting that E. coli was able to express and produce the chitinase enzyme to the soil even as the population was gradually decreasing. Because the majority of the strains was alive for only a short period of time and the Fusarium-affected seedlings showed symptoms of wilting within 7-10 days, it seems that the pathogen control was decided early after the introduction of the biocontrol agent, eliminating the survival of the antagonist. These results indicated that soil inoculated E. coli could sufficiently express and produce the recombinant protein to control the pathogen, and root or soil colonization of the antagonist might not be a significant factor in determining the efficacy of biological control.
Chung
Soohee
S
Department of Applied Microbiology, College of Natural Resources, Yeungnam University, Gyeongsan 712-749, Korea.
Kim
Sang-Dal
SD
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D002688:Chitinases / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D015169:Colony Count, Microbial; D018553:Cucumis sativus / Q000382:microbiology*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005670:Fusarium / Q000254:growth & development* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D036226:Seedlings / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012705:Serratia / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D012988:Soil Microbiology
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D002688:Chitinases