Measurement of glycine in human brain by triple refocusing 1H-MRS in vivo at 3.0T.
A new (1)H-MRS filtering strategy for selective measurement of glycine (Gly) in human brain in vivo at 3.0T is proposed. Investigation of multiple refocusing following a 90 degrees excitation pulse indicated that triple refocusing is most effective for suppression of the strongly coupled resonances of myo-inositol (mI) at the Gly 3.55-ppm resonance. The echo times of the triple refocusing were optimized, with numerical analysis of the filtering performance, as {TE(1), TE(2), TE(3)} = {67, 62, 69} ms. Compared with the 90 degrees -acquired mI signal the mI suppression ratios of the filter were 170 and 1000, in terms of peak amplitude and area, respectively, between 3.51 and 3.59 ppm. From LCModel analyses, using density-matrix calculated spectra as basis functions, the concentration of Gly in parieto-occipital cortex of healthy adults was estimated to be 0.5 +/- 0.1 mM (mean +/- SD, n = 6), with reference to creatine at 8 mM.
Choi
Changho
C
Department of Biomedical Engineering, University of Alberta, Edmonton, Alberta, Canada. Changho.Choi@UTSouthwestern.edu
Bhardwaj
Paramjit P
PP
Seres
Peter
P
Kalra
Sanjay
S
Tibbo
Philip G
PG
Coupland
Nicholas J
NJ
D000328:Adult; D001921:Brain / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism*; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007294:Inositol / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D019047:Phantoms, Imaging
D007294:Inositol; D005998:Glycine