Carbohydrate accumulation and utilization by oocytes of Rhodnius prolixus.
The processes of accumulation and mobilization of carbohydrate stores in eggs of Rhodnius prolixus were analyzed. During oogenesis, the total amounts of glycogen, glucose, and trehalose increased with an accumulation of proteins, especially when oocytes grew from 1.0 to 1.5 mm in length. At 2.0 mm length, when oocytes were ready for oviposition, nutrient reserves did not increase appreciably and trehalose content decreased. Mating did not affect the final content of carbohydrates or proteins in oocytes of mated and virgin females. A trehalase activity was detected in follicles containing vitellogenic oocytes, 1.0 and 1.5 mm length, in both mated and virgin females. This activity was extremely low in chorionated, 2.0-mm oocytes. After oviposition, glycogen content decreased in fertilized eggs, but not in unfertilized ones, and some was present in newly hatched nymphs. Glucose content remained constant in unfertilized eggs, but increased in fertilized ones, while total protein amount was constant in both groups after egg laying.
Instituto de Bioquímica Médica, Universidade Federal do Rio Janeiro, Rio de Janeiro, Brazil.
Rachel
R
Santos
Ana C
AC
Mariano
Rafael
R
Rosas-Oliveira
Bernardo
B
Pascarelli
Ednildo A
EA
Machado
José R
JR
Meyer-Fernandes
Katia C
KC
Gondim
D000818:Animals; D050260:Carbohydrate Metabolism / Q000502:physiology*; D004527:Egg Proteins / Q000032:analysis; D004625:Embryo, Nonmammalian / Q000737:chemistry; D005260:Female; D005947:Glucose / Q000032:analysis; D006003:Glycogen / Q000032:analysis; D008297:Male; D009865:Oocytes / Q000737:chemistry / Q000378:metabolism*; D009866:Oogenesis / Q000502:physiology*; D006080:Ovarian Follicle / Q000737:chemistry; D010058:Oviposition / Q000502:physiology; D012239:Rhodnius / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors; D014198:Trehalase / Q000032:analysis; D014199:Trehalose / Q000032:analysis; D015053:Zygote / Q000737:chemistry / Q000378:metabolism*
D004527:Egg Proteins; D006003:Glycogen; D014199:Trehalose; D014198:Trehalase; D005947:Glucose