Lipopolysaccharide induced expression of pentraxin 3 in human neutrophils and monocyte-derived macrophages.
Pentraxin 3 (PTX3) is a prototype protein of long pentraxin. PTX3 is produced by various cells, such as monocytes/macrophages (Mphis) in response to lipopolysaccharide (LPS) and proinflammatory signals. We performed immunoblotting, immunohistochemical staining, reverse transcriptase-polymerase chain reaction (RT-PCR), quantitative real-time PCR and enzyme-linked immunosorbent assay (ELISA) of PTX3 in human monocyte-derived Mphis and neutrophils. PTX3 expression was observed in the cytoplasm of both GM-CSF induced monocyte-derived Mphi (GM-Mphi) and M-CSF induced monocyte-derived Mphi (M-Mphi). PTX3 level in both Mphis was up-regulated at 24 h after LPS stimulation. Moreover, we confirmed PTX3 expression in freshly isolated neutrophils, and PTX3 level was distinctly up-regulated at 6 and 24 h after LPS stimulation. These findings suggested that PTX3 expression, not only in Mphis, but also in neutrophils, may reflect the role of PTX3 in inflammation. We believe that PTX3 can contribute as a diagnostic tool to evaluate inflammation at peripheral sites.
Division of Cellular and Molecular Pathology, Department of Cellular Function, Niigata University Graduate School of Medical and Dental Sciences, Asahimachi 1-757, Chuou-ku, Niigata 951-8510, Japan.
Masaru
M
Imamura
Takashi
T
Kawasaki
Alexander S
AS
Savchenko
Riuko
R
Ohashi
Shuying
S
Jiang
Kyoko
K
Miyamoto
Yukio
Y
Ito
Hiroko
H
Iwanari
Mina
M
Sagara
Toshiya
T
Tanaka
Takao
T
Hamakubo
Tatsuhiko
T
Kodama
Makoto
M
Uchiyama
Makoto
M
Naito
D000818:Animals; D002097:C-Reactive Protein / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D000683:Serum Amyloid P-Component / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D000099153:Pentraxins
D002097:C-Reactive Protein; D008070:Lipopolysaccharides; D000683:Serum Amyloid P-Component; D000099153:Pentraxins