Supramolecular systems-based HPLC for chiral separation of beta-adrenergics and beta-adrenolytics in drug discovery schemes.
Increasing amount of data considering polymorphism, splice variants and various affinity states of beta-adrenoceptors has resulted in a new range of opportunities for enantiopure beta-adrenergic and beta-adrenolytic drug discovery and continuous development of reliable high-throughput screening procedures enabling tissue specific pharmacological evaluation of these drugs. Design and fast pharmacological profiling of single enantiomeric molecules combining beta-adrenoceptor affinity with other pharmacophores is also still challenging ability. As the use of chiral stationary phases in HPLC has particularly benefited from results of supramolecular chemistry, this review summarises recent achievements provided by this technique in deciphering of enatiorecognition phenomena affecting pharmacological selectivity of beta-adrenergics and beta-adrenolytics and modifying the efficiency of currently proposed beta-adrenoceptor-targeted therapies. Detailed characteristic of chiral separation performance of these drugs in the range of available supramolecular HPLC systems has also been presented.
Department of Chemistry, Jamia Millia Islamia, Jamia Nagar, New Delhi-110025, India.
Imran
I
Ali
Afzal
A
Hussain
Hassan Y
HY
Aboul-Enein
Grzegorz
G
Bazylak
D000318:Adrenergic beta-Agonists / Q000737:chemistry* / Q000302:isolation & purification; D000319:Adrenergic beta-Antagonists / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000737:chemistry / Q000302:isolation & purification; D004304:Dosage Forms; D006020:Glycopeptides / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D011134:Polysaccharides / Q000737:chemistry; D012997:Solvents; D013237:Stereoisomerism; D013696:Temperature
D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D003505:Cyclodextrins; D004304:Dosage Forms; D006020:Glycopeptides; D007202:Indicators and Reagents; D011134:Polysaccharides; D012997:Solvents