Involvement of extracellular matrix and integrin-like proteins on conidial adhesion and appressorium differentiation in Magnaporthe oryzae.
Conidial adhesion and appressorium formation of Magnaporthe oryzae on the rice surface are important early events in the infection process. As an initiative step to understand the mechanisms underlying these cellular processes at a biochemical level, the effect of a human fibronectin antibody (HFA) and RGD peptides on conidial adhesion and appressorium formation was evaluated. HFA inhibited conidial adhesion and appressorium formation in a dosage-dependent manner. RGD peptides also inhibited these cellular events. Conidial adhesion and appressorium formation inhibited by RGD peptides were restored by chemicals involved in the cyclic AMP-dependent signaling pathway. These results suggest that extracellular matrix proteins might be involved in conidial adhesion and appressorium formation through integrin-like receptor mediation and modulation of cAMP-dependent signaling in the cells.
Bae
Cheol-Yong
CY
Department of Agricultural Biotechnology, Center for Fungal Genetic Resources, Seoul National University, Seoul 151-921, Korea.
Kim
Soonok
S
Choi
Woo Bong
WB
Lee
Yong-Hwan
YH
D000906:Antibodies / Q000494:pharmacology; D002448:Cell Adhesion; D005109:Extracellular Matrix / Q000378:metabolism*; D005353:Fibronectins / Q000494:pharmacology; D005656:Fungal Proteins / Q000378:metabolism*; D056229:Fungal Structures / Q000502:physiology*; D016023:Integrins / Q000502:physiology*; D020082:Magnaporthe / Q000737:chemistry* / Q000166:cytology*; D009842:Oligopeptides / Q000494:pharmacology; D015398:Signal Transduction; D013172:Spores, Fungal / Q000502:physiology*; D013499:Surface Properties; D013997:Time Factors
D000906:Antibodies; D005353:Fibronectins; D005656:Fungal Proteins; D016023:Integrins; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid