Retinal cell types are differentially affected in sheep with scrapie.
Transmissible spongiform encephalopathies (TSEs) are a group of fatal neurodegenerative diseases characterized microscopically by spongiform lesions (vacuolation) in the neuropil, neuronal loss, and gliosis. Accumulation of the abnormal form of the prion protein (PrP(Sc)) has been demonstrated in the retina of natural and non-natural TSE-affected hosts, with or without evidence of microscopically detectable retinal pathology. This study was conducted to investigate the effect of PrP(Sc) accumulation on retinal neurons in a natural host lacking overt microscopical evidence of retinal degeneration by comparing the distribution of retinal cell type-specific markers in control and scrapie-affected sheep. In retinas with PrP(Sc)-immunoreactivity, there was disruption of the normal immunoreactivity patterns of the alpha isoform of protein kinase C (PKCalpha) and vesicular glutamate transporter 1 (VGLUT1), markers of retinal bipolar cells. Altered immunoreactivity was also observed for microtubule-associated protein 2 (MAP2), a marker of a subset of retinal ganglion cells, and glutamine synthetase (GS), a marker of Müller glia. These results demonstrate alterations of immunoreactivity patterns for proteins associated with specific cell types in retinas with PrP(Sc) accumulation, despite an absence of microscopical evidence of retinal degeneration.
Smith
J D
JD
Department of Biomedical Sciences, Iowa State University, Ames, Iowa 50010, USA.
Greenlee
J J
JJ
Hamir
A N
AN
West Greenlee
M H
MH
D000818:Animals; D005974:Glutamate-Ammonia Ligase / Q000096:biosynthesis; D007150:Immunohistochemistry; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis; D018620:PrPSc Proteins / Q000032:analysis / Q000378:metabolism*; D051571:Protein Kinase C-alpha / Q000096:biosynthesis; D051245:Retinal Bipolar Cells / Q000378:metabolism* / Q000473:pathology; D012165:Retinal Ganglion Cells / Q000378:metabolism* / Q000473:pathology; D012608:Scrapie / Q000378:metabolism / Q000503:physiopathology*; D012756:Sheep; D050598:Vesicular Glutamate Transport Protein 1 / Q000096:biosynthesis
D008869:Microtubule-Associated Proteins; D018620:PrPSc Proteins; D050598:Vesicular Glutamate Transport Protein 1; D051571:Protein Kinase C-alpha; D005974:Glutamate-Ammonia Ligase