In vitro and in vivo effects of Ranunculus peltatus subsp. baudotii methanol extract on models of eicosanoid production and contact dermatitis.
Ranunculus (Crowfoot) species are numerous and they are all reputed to be counter-irritants and are used in several topical conditions. In order to study the pharmacological mechanisms of action underlying this popular use, a methanol extract of Ranunculus peltatus was tested in vitro in various assays involving eicosanoid and human elastase release by intact cells as well as in vivo, with models of delayed-type hypersensitivity (DTH) contact dermatitis. The extract proved to be a selective inhibitor of the cyclooxygenase-1 pathway, producing the total inhibition of 12-(S)-HHTrE release at 200 microg/mL, while leaving both 5-lipoxygenase and 12-lipoxygenase activities unaffected at the same dose. The n-hexane, chloroform and ethyl acetate fractions of the crude methanol extract inhibited LTB(4) release by intact rat peritoneal neutrophils, but more polar fractions were inactive and did not increase the 5-LOX activity as seen previously for extracts of other Ranunculus species. In the in vivo models, the methanol extract reduced the dinitrofluorobenzene (DNFB)-induced oedema by 40%, but failed to inhibit the oedema brought on by oxazolone. The results agree with the age-old assertion that Water Crowfoot species can be used as a topical antiinflammatory remedy without the prominent irritant action that accompanies the application of non-aquatic Ranunculus species.
Departament de Farmacologia, Facultat de Farmàcia, Universitat de València, Av. Vicent Andrés Estellés, s/n. 46100 Burjassot, València, Spain. jose.prieto@pharmacy.ac.uk
J M
JM
Prieto
M C
MC
Recio
R M
RM
Giner
G R
GR
Schinella
S
S
Máñez
J L
JL
Ríos
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D051545:Cyclooxygenase 1 / Q000187:drug effects / Q000378:metabolism; D017449:Dermatitis, Allergic Contact / Q000188:drug therapy*; D015777:Eicosanoids / Q000096:biosynthesis*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004798:Enzymes / Q000032:analysis / Q000187:drug effects; D005260:Female; D006801:Humans; D000432:Methanol / Q000737:chemistry; D051379:Mice; D023421:Models, Animal; D009504:Neutrophils / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D031947:Ranunculus / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D015777:Eicosanoids; D004791:Enzyme Inhibitors; D004798:Enzymes; D010936:Plant Extracts; D051545:Cyclooxygenase 1; D000432:Methanol