Induction of growth hormone release by dioscin from Dioscorea batatas DECNE.
In this study, dioscin was isolated from Dioscoreae Rhizoma (DR), which is the rhizome of Dioscorea batatas D(ECNE). that inhabits broad areas of Korea and Japan. To determine whether dioscin induced growth hormone (GH) release, we evaluated its induction effects on GH release both in vitro and in vivo. The 70% methanol extract of DR, and its n-hexane and n-BuOH fractions, induced rat GH (rGH) release in rat pituitary cells 10-fold, 8-fold, and 5- fold higher than the control (0.36 +/- 0.02 nM), respectively (p < 0.05 each). The dioscin-induced rGH release of the cells was concentration-dependent and its ED(50) was 1.14 x 10(-5) M. Within 90 minutes after intravenous administration of 10 microg/kg (p < 0.05 at t(max)), dioscin caused the greatest increase in rGH concentration (C(max)) in the rat plasma (34.16 +/- 14.10 ng/ml) (n = 4), which was twice as high as the control group (12.88 +/- 3.29 ng/ml) (n = 27).
Lee
Ho Young
HY
Department of Drug Research & Development, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
Jung
Dae Young
DY
Ha
Hyekyung
H
Son
Kun-Ho
KH
Jeon
Su-Jin
SJ
Kim
Chungsook
C
D000818:Animals; D002478:Cells, Cultured; D027742:Dioscorea / Q000737:chemistry*; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D013006:Growth Hormone / Q000378:metabolism*; D007723:Korea; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley; D018013:Receptors, Neuropeptide / Q000378:metabolism; D018017:Receptors, Pituitary Hormone-Regulating Hormone / Q000378:metabolism
D018013:Receptors, Neuropeptide; D018017:Receptors, Pituitary Hormone-Regulating Hormone; C019357:dioscin; D013006:Growth Hormone; C048250:somatotropin releasing hormone receptor; D004144:Diosgenin