[Cloning and sequence analysis of alpha satellite DNA in Arctic fox].
Genomic DNAs from Arctic fox were cleaved with restriction enzymes. The prominent ethidium-bromide stanined bands were cloned and sequenced after separation of DNA fragments by agarose gel electrophoresis. Forty-two satellite sequences were obtained by sequencing. Sequence analyses revealed that the satellite monomer in length was 737 bp, with an average G+C content of 51.9%. There were 91%-97% homology among the monomers, and the monomer consisted of three subrepeats of approximately 245 bp in length with 51%-54% homology. The satellite DNAs were specific for canidae, and had 74% homology to the dog centromere-associated nucleotide sequences. In order to distinguish from another satellite DNAs of canidae genome, the satellite was designated as alpha satellite DNA.
Yan
Shou-Qing
SQ
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China. ysq2004927@163.com
Zhu
Wan-Ju
WJ
Zhang
Xue-Mei
XM
Li
Bing
B
Sun
Jin-Hai
JH
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004276:DNA, Satellite / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005589:Foxes / Q000235:genetics*; D023281:Genomics; D008297:Male; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004276:DNA, Satellite; D004262:DNA Restriction Enzymes