Unambiguous structural elucidation of base-modified purine nucleosides using NMR.
A general and unambiguous approach has been developed for structural elucidation of modified purine nucleosides using NMR spectroscopy. Systematic assignment of proton and carbon signals of modified nucleosides was firmly established by COSY and the anomerism of the glycosidic linkage of synthetic nucleosides clearly elucidated by NOESY experiments. Characteristic properties of 15N-isotopic labelling at specific positions of nucleosides were also employed for structural studies. The reported approach is applicable to other modified nucleosides and nucleotides, as well as nucleobases.
Department of Chemistry, Open University, Walton Hall, Milton Keynes MK7 6AA, UK.
Raman
R
Narukulla
David E G
DE
Shuker
Vasudevan
V
Ramesh
Yao-Zhong
YZ
Xu
D002241:Carbohydrates / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009690:Nucleic Acid Conformation; D011522:Protons; D011684:Purine Nucleosides / Q000737:chemistry*; D011685:Purine Nucleotides / Q000737:chemistry*
D002241:Carbohydrates; D011522:Protons; D011684:Purine Nucleosides; D011685:Purine Nucleotides