High-density fermentation of microalga Chlorella protothecoides in bioreactor for microbio-diesel production.
Agal-fermentation-based microbio-diesel production was realized through high-cell-density fermentation of Chlorella protothecoides and efficient transesterification process. Cell density achieved was 16.8 g l(-1) in 184 h and 51.2 g l(-1) in 167 h in a 5-l bioreactor by performing preliminary and improved fed-batch culture strategy, respectively. The lipid content was 57.8, 55.2, and 50.3% of cell dry weight from batch, primary, and improved fed-batch culture in 5-l bioreactor. Transesterification was catalyzed by immobilized lipase, and the conversion rate reached up to 98%. The properties of biodiesel from Chlorella were comparable to conventional diesel fuel and comply with US standard for Biodiesel. In a word, the approach including high-density fermentation of Chlorella and enzymatic transesterification process were set up and proved to be a promising alternative for biodiesel production.
Xiong
Wei
W
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, People's Republic of China.
Li
Xiufeng
X
Xiang
Jinyi
J
Wu
Qingyu
Q
D018533:Biomass; D019149:Bioreactors; D002708:Chlorella / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D005285:Fermentation; D005742:Gasoline; D008049:Lipase / Q000378:metabolism; D008055:Lipids / Q000032:analysis; D009821:Oils / Q000378:metabolism*; D013997:Time Factors; D014481:United States
D004800:Enzymes, Immobilized; D005227:Fatty Acids; D005742:Gasoline; D008055:Lipids; D009821:Oils; D008049:Lipase