[Effects on T cell proliferation by culture supernatant of fibroblast cells modified with mIDO gene].
OBJECTIVE
To investigate the effects on T cell proliferation by culture supernatant of fibroblast cells modified by mIDO gene.


METHODS
Eukaryotic expression plasmid pEGFP-Nl-mlDO was transfected into the mouse fibroblast cell lines by liposome. The concentration of tryptophan in pEGFP-Nl-mIDO fibroblast cell culture medium was tested. The effects on ConA-stimulated T cells, proliferation by pEGFP-Nl-mlDO fibroblast cell culture medium were analyzed by CFSE technique.


RESULTS
pEGFP-N1-mlDO was transfected into the fibroblasts cell lines successfully. Tryptophan in pEGFP-Nl-mIDO-transfected-fibroblast cell culture medium was undetectable, which showed pEGFP-Nl-mIDO-transfected-fibroblast cells produced active mIDO, resulting in the degradation of tryptophan in the medium. mIDO could inhibit proliferation of ConA-stimulated T cells, which was marked by CFSE.


CONCLUSIONS
T cell proliferation can be inhibited by the fibroblast cells modified by mIDO gene.
Luo
Lu
L
Department of Obstetrics and Gynecology, the Second Affiliated Hospital, Zhongshan University, Guangzhou 510120, China. jessicaluolu@163.com
Zeng
Yao-Ying
YY
Wang
Zhen
Z
Wang
Qing
Q
Zhao
Li
L
Yang
Dong-Zi
DZ
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D013388:Succinimides / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D014162:Transfection
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D003470:Culture Media; D005452:Fluoresceins; D005456:Fluorescent Dyes; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D013388:Succinimides; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins