Transposons as tools for enhancer trap screens in vertebrates.
DNA transposons are efficient tools in transgenesis and have therefore become popular in the analysis of the regulatory genome in vertebrates via enhancer trap screens. Here, I discuss recent progress in this field of research, with a focus on the application of one of these transposons, namely the medaka fish derived Tol2, to enhancer trapping in zebrafish, and how this approach compares with others that have a similar objective.
Korzh
Vladimir
V
Institute of Molecular and Cell Biology, Biopolis Dr, Proteos, 138673, Singapore. vlad@imcb.a-star.edu.sg
D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D011401:Promoter Regions, Genetic; D014714:Vertebrates / Q000235:genetics*; D015027:Zebrafish
D004251:DNA Transposable Elements