[Relationship between alpha- and beta-isoform of corticosteroid receptors and corticosteroid resistant allergic rhinitis].
OBJECTIVE
To clarify the relationship between the expression of alpha- and beta-isoform of corticosteroid receptors (CS) in peripheral blood mononuclear cell (PBMC) and response to corticosteroid in patients with allergic rhinitis (AR).


METHODS
Semi-quantitative RT-PCR was used to detect the expression of CS-alpha, beta in PBMC in patients with AR and to observe the different responses to corticosteroid in controls. Immunocytochemical assay was used to detect the expression of protein of CS-alpha and CS-beta.


RESULTS
1) The expression of CS-alpha mRNA was detected in the sensitive group and the resistant group of patients with AR and the controls with CS-alpha/GAPDH mRNA (x +/- s) 1.15 +/- 0.75, 1.63 +/- 0.78, 1.27 +/- 0.51 respectively. 2) The expression of CS-beta mRNA in PBMC in the resistant group of patients with AR was significantly higher than that in the sensitive group and the controls (P < 0.05), with CS-beta/GAPDH mRNA 1.42 +/- 0.73, 0.82 +/- 0.59, 0.80 +/- 0.68 respectively. 3) The number of CS-beta-positive PBMC in the resistant group was significantly higher than that in the sensitive group and the controls (P < 0.01), with the number of CS-beta-positive PBMC 28.8% +/- 9. 9%, 5.9% +/- 3.2%, 5.5% +/- 6.8% respectively.


CONCLUSIONS
It is shown that the excessive expression of CS-beta may serve as a novel predictor of corticosteroid resistance in patients with AR.
Luo
Hong
H
Department of Otorhinolaryngology, Affiliated Xiangfan Hospital, Tongji Medical College, Huazhong University of Science and Technology, The Central Hospital of Xiangfan City, Xiangfan 441021, China. lh07103999370@163.com
Yan
Neng-bing
NB
Zeng
Ping-fan
PF
Liang
Jin-jun
JJ
Wu
Guo-hui
GH
Ke
Sai-xiong
SX
Wang
Peng-ju
PJ
Wang
Jin-yun
JY
D000293:Adolescent; D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D004351:Drug Resistance; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D020033:Protein Isoforms / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011987:Receptors, Steroid / Q000378:metabolism*; D012221:Rhinitis, Allergic, Perennial / Q000188:drug therapy / Q000378:metabolism*; D055815:Young Adult
D000305:Adrenal Cortex Hormones; D020033:Protein Isoforms; D012333:RNA, Messenger; D011987:Receptors, Steroid