Molecular assembly of Zn porphyrin complexes using synthetic light-harvesting model polypeptides.
Synthetic single alpha-helix hydrophobic polypeptides, which have similar amino acid sequences to the hydrophobic core in the native light-harvesting 1-beta polypeptide from Rhodobacter sphaeroides, formed Zn porphyrin complexes on a gold electrode, as well as in n-octyl-beta-glucoside micelles: this process is dependent on the structure of the pigments and the polypeptides. Interestingly, an enhanced photoelectric current was observed when Zn mesoporphyrin monomer complexed with the synthetic light-harvesting model polypeptide in an alpha-helical configuration was assembled with a defined orientation onto the electrode. Analog of these light-harvesting model complexes are also useful in providing insights into the effect of polypeptide structure on the formation of light-harvesting complexes on and off electrodes.
Ochiai
Tsuyoshi
T
Materials Science and Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya, 466-8555, Japan.
Asaoka
Takahide
T
Kato
Tomoya
T
Osaka
Shinichiro
S
Dewa
Takehisa
T
Yamashita
Keiji
K
Gardiner
Alastair T
AT
Hashimoto
Hideki
H
Nango
Mamoru
M
D000595:Amino Acid Sequence; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry*; D008665:Metalloporphyrins / Q000737:chemistry*; D008969:Molecular Sequence Data; D012242:Rhodobacter sphaeroides / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D013057:Spectrum Analysis / Q000379:methods
D045342:Light-Harvesting Protein Complexes; D008665:Metalloporphyrins; C017802:zinc hematoporphyrin; D006046:Gold