Positive correlation between chymase-like angiotensin II-forming activity in mononuclear cells and serum cholesterol level.
OBJECTIVE
The local renin-angiotensin system is important in cardiovascular diseases. The present study examined the association between angiotensin (Ang) II-forming activity in fractionated peripheral leukocytes and atherosclerotic risks such as blood pressure, smoking, age and serum cholesterol level, and used a new analytical approach for the measurement of chymase-like activity in peripheral blood to assess the relationship between the chymase-like activities in leukocytes and atherosclerotic risks.


METHODS
Peripheral blood samples were obtained from normal and high blood pressure patients in the presence or absence of ischemic heart disease. Mononuclear cell or polymorphonuclear cell fraction of leukocyte was isolated by centrifugation with either Lymphoprep or Polymorphprep, respectively. Chymase-like, angiotensin converting enzyme, and cathepsin G-dependent Ang II-forming activities in the homogenates of mononuclear cell or polymorphonuclear cell fraction were measured using Ang I as a substrate.


RESULTS
The chymase-like Ang II-forming activity in the mononuclear cell fraction slightly or significantly increased in non-smoker patients with high blood pressure (systolic and diastolic blood pressure, p = 0.11; mean blood pressure, p < 0.05). Chymase-like Ang II-forming activity in the mononuclear cell fraction positively correlated with serum total cholesterol (p < 0.05) level.


CONCLUSIONS
Our data indicates that chymase in mononuclear cells from peripheral blood is activated by high blood pressure or hypercholesterolemia.
Murakami
Kinshiro
K
Department of Cardiology, Fukuoka University Faculty of Medicine, Fukuoka.
Uehara
Yoshinari
Y
Abe
Satomi
S
Inoue
Yukiko
Y
Ideishi
Munehito
M
Saku
Keijiro
K
Urata
Hidenori
H
D000804:Angiotensin II / Q000096:biosynthesis*; D001161:Arteriosclerosis / Q000097:blood*; D002784:Cholesterol / Q000097:blood*; D053818:Chymases / Q000378:metabolism*; D006801:Humans; D006973:Hypertension / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D008875:Middle Aged; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects
D000804:Angiotensin II; D002784:Cholesterol; D053818:Chymases