EBV-1 and HCMV in aggressive periodontitis in Brazilian patients.
The purpose of the present investigation was to compare the presence of Epstein-Barr virus type 1 (EBV-1) and of Human Cytomegalovirus (HCMV) in crevicular fluid samples from deep and shallow periodontal pocket sites of Brazilian patients with aggressive periodontitis. A total of 30 systemically healthy patients with aggressive periodontitis participated in the study. Paper points were inserted into 2 gingivitis sites (<3 mm) and into 2 periodontitis sites (>5 mm) in each patient. PCR assay was used to identify genomic copies of HCMV and EBV-1. Twenty-three patients (77%) were positive for EBV-1, while only 2 patients (6%) were positive for HCMV. The McNemar test revealed a positive association between EBV-1 and periodontal lesions (p=0.043). Thirty-four (57%) out of 60 periodontitis sites were positive for EBV-1, whereas 18 (30%) gingivitis sites were positive (p=0.01). Only two sites (6.7%) were positive for HCMV. No positive association was found between HCMV and periodontitis or gingivitis (p=0.479). The elevated occurrence of EBV-1 DNA in periodontal pockets of patients with aggressive periodontitis supports a possible periodontopathic role of this virus.
Department of Oral Surgery and Pathology, School of Dentistry, Federal University of Minas Gerais, Brazil.
Soraia Almeida
SA
Watanabe
Jeane de Fátima
Jde F
Correia-Silva
Martinho Campolina Rebello
MC
Horta
José Eustáquio da
JE
Costa
Ricardo Santiago
RS
Gomez
D000293:Adolescent; D000328:Adult; D001938:Brazil; D016009:Chi-Square Distribution; D003587:Cytomegalovirus / Q000302:isolation & purification / Q000472:pathogenicity*; D003586:Cytomegalovirus Infections; D004279:DNA, Viral / Q000302:isolation & purification; D020031:Epstein-Barr Virus Infections; D005260:Female; D005881:Gingiva / Q000821:virology; D005891:Gingivitis / Q000821:virology; D004854:Herpesvirus 4, Human / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000821:virology; D010518:Periodontitis / Q000473:pathology / Q000821:virology*; D012720:Severity of Illness Index
D004279:DNA, Viral