Continuous counter current extraction, isolation and determination of solanesol in Nicotiana tobacum L. by non-aqueous reversed phase high performance liquid chromatography.
A method of continuous counter current extraction in a large-scale production of solanesol from tobacco leaves was developed. The crude extract containing 15-20% solanesol was subjected to a series of steps, viz., saponification, solvent recrystallization and column chromatography. The pure material was characterized by FT-IR, ESI-MS, 1H NMR and 13C NMR spectrometry. The analysis was carried out by a simple and rapid non-aqueous reversed-phase high performance liquid chromatographic method on a Hypersil BDS C18 column (250 mm x 4.6mm, particle size 5 microm) with isopropyl alcohol-methanol (60:40, v/v) as mobile phase and detection at 215 nm. The product purity was between 95 and 98% (w/w) as determined by HPLC.
Rao
R Nageswara
RN
Analytical Chemistry Division, Indian Institute of Chemical Technology, Hyderabad 500 007, India. rnrao55@yahoo.com
Talluri
M V N Kumar
MV
Krishna
T S V N Murali
TS
Ravindranath
K
K
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013729:Terpenes / Q000032:analysis / Q000302:isolation & purification*; D014026:Nicotiana / Q000737:chemistry*
D013729:Terpenes; C017719:solanesol