Self-assembling cyclodextrin based hydrogels for the sustained delivery of hydrophobic drugs.
This study aims to investigate the rheological properties of self-assembling gels containing cyclodextrins with potential application as injectable matrix for the sustained delivery of poorly soluble drugs. The ability of these gels to entrap two hydrophobic molecules, benzophenone (BZ) and tamoxifen (TM), and to allow their in vitro sustained release was evaluated. In view of their future pharmaceutical use, gels were sterilized by high hydrostatic pressures (HHP) and tested for their biocompatibility. The gels formed instantaneously at room temperature, by mixing the aqueous solutions of two polymers: a beta-cyclodextrin polymer (pbetaCD) and a hydrophobically modified dextran by grafting alkyl side chains (MD). MD-pbetaCD gels presented a viscoelastic behavior under low shear, characterized by constant values of the loss modulus G'' and the storage modulus G'. The most stable gels were obtained for a total polymer concentration C(p) of 6.6% and 7.5% (w/w), and a polymer ratio MD/pbetaCD of 50/50 and 33/67 (w/w). BZ and TM were successfully incorporated into MD-pbetaCD gels with loading efficiencies as high as 90%. In vitro, TM and BZ were released gradually from the gel matrix with less than 25% and 75% release, respectively, after 6 days incubation. HHP treatment did not modify the rheological characteristics of MD-pbetaCD gels. Moreover, the low toxicity of these gels after intramuscular administration in rabbits makes them promising injectable devices for local delivery of drugs.
UMR CNRS 8612, Faculté de Pharmacie, Université Paris XI, Châtenay-Malabry, France.
S
S
Daoud-Mahammed
J L
JL
Grossiord
T
T
Bergua
C
C
Amiel
P
P
Couvreur
R
R
Gref
D000818:Animals; D001577:Benzophenones / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000378:metabolism; D001696:Biomechanical Phenomena; D003911:Dextrans / Q000737:chemistry; D016503:Drug Delivery Systems; D020100:Hydrogels / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D006874:Hydrostatic Pressure; D008422:Materials Testing; D046529:Microscopy, Electron, Transmission; D011506:Proteins / Q000494:pharmacology; D011817:Rabbits; D012212:Rheology; D012492:Salts / Q000494:pharmacology; D012867:Skin / Q000166:cytology; D013629:Tamoxifen / Q000494:pharmacology*; D047392:beta-Cyclodextrins / Q000737:chemistry*
D001577:Benzophenones; D001672:Biocompatible Materials; D003911:Dextrans; D020100:Hydrogels; D011506:Proteins; D012492:Salts; D047392:beta-Cyclodextrins; D013629:Tamoxifen; C047723:benzophenone; C031215:betadex