Anti-oxidant effect of ascorbic and dehydroascorbic acids in hippocampal slice culture.
Ascorbic acid (AA) and dehydroascorbic acid (DHA) have been shown to have protective effects as anti-oxidants in experimental neurological disorder models such as stroke, ischemia, and epileptic seizures. The present study was conducted to examine the protective effects of AA and DHA on kainic acid (KA) neurotoxicity using organotypic hippocampal slice cultures. After 12h KA treatment, significant delayed neuronal death was detected in the CA3, but not the CA1, region. Pretreatment with intermediate doses of AA and DHA significantly prevented cell death and inhibited reactive oxygen species (ROS) level, and mitochondrial dysfunction in the CA3 region. In contrast, pretreatment with low or high doses of AA or DHA was not effective. These data suggest that pretreatment with both AA and DHA has dose-dependent neuroprotective effects on KA-induced neuronal injury through inhibiting ROS generation and mitochondrial dysfunction.
Kim
Eun Jin
EJ
Department of Physiology, Brain Research Institute, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, C.P.O. Box 8044, Seoul 120-752, Republic of Korea.
Won
Ran
R
Sohn
Jin-Hun
JH
Chung
Myung-Ae
MA
Nam
Taick Sang
TS
Lee
Hye-Jung
HJ
Lee
Bae Hwan
BH
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D017209:Apoptosis / Q000187:drug effects; D001205:Ascorbic Acid / Q000008:administration & dosage*; D003683:Dehydroascorbic Acid / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*
D000975:Antioxidants; D017382:Reactive Oxygen Species; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid