Development of genomic microsatellite markers in Coffea canephora and their transferability to other coffee species.
Of the 103 accepted Coffea species, 70% are threatened with extinction but only a few of them have been studied. A set of 40 polymorphic microsatellite markers was developed using a GA/GT-enriched Coffea canephora genomic library. Amplification of these markers was tested in accessions of C. heterocalyx (a Critically Endangered species) and C. pseudozanguebariae (a Vulnerable species) belonging to different African geographical clades. All microsatellites were polymorphic in C. canephora, with a mean allele number per polymorphic locus of more than 3 (at least 9 genotypes were tested). Observed and expected heterozygosities calculated for C. canephora and C. pseudozanguebariae varied from 0.10 to 0.91 and from 0.20 to 0.77, respectively. In total, 38 primer pairs (95%) were amplified in C. heterocalyx and C. pseudozanguebariae, indicating their high level of transferability across the genus Coffea. This large marker set will be useful for more extensive genetic studies of threatened Coffea species.
Poncet
Valérie
V
IRD, UMR DIA-PC, 911 avenue Agropolis, BP 64501, 34394 Montpellier cedex 5, France. poncet@mpl.ird.fr
Dufour
Magali
M
Hamon
Perla
P
Hamon
Serge
S
de Kochko
Alexandre
A
Leroy
Thierry
T
D000483:Alleles; D040503:Coffea / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D018745:Genome, Plant; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
D005819:Genetic Markers