A simple and rapid HPLC method for the detection of glyburide in plasma original research communication (analytical).
BACKGROUND
Although a variety of HPLC methods have been previously described for the quantification of glyburide, attempts to implement them clinically have been unsuccessful. Some are time consuming, expensive, or not directly applicable to human plasma. Others are outdated, as the necessary materials are no longer available.


OBJECTIVE
To describe a simple, rapid, and sensitive HPLC method for the analysis of human plasma and perfusate.


METHODS
Samples were extracted by liquid-liquid extraction with chloroform at neutral pH. Glyburide was detected at 254 nm, with a total run time of 7 min per sample.


RESULTS
Standard curves of 50 to 400 ng/mL of glyburide were linear (r(2)=0.998). Inter-day and intra-day sample coefficient of variations were 8% and 4%, respectively. Recoveries ranged from 71 to 75% in human plasma samples for the 20-400 ng/mL concentration range.


CONCLUSIONS
Clinically, this method can be applied in the therapeutic drug monitoring of glyburide.
University of Toronto, Faculty of Medicine, Department of Pharmacology, Toronto, Ontario, Canada.
Christelle
C
Gedeon
Bhushan
B
Kapur
Katarina
K
Aleksa
Gideon
G
Koren
D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016640:Diabetes, Gestational / Q000097:blood; D016903:Drug Monitoring / Q000379:methods; D005260:Female; D005905:Glyburide / Q000097:blood*; D006801:Humans; D007004:Hypoglycemic Agents / Q000097:blood*; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D053719:Tandem Mass Spectrometry
D007004:Hypoglycemic Agents; D005905:Glyburide