Fast characterization of industrial soy protein isolates by direct analysis with matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
Industrial soy protein isolates (SPIs) due to differences in their processing conditions may differ both in composition and in degree of hydrolysis. As a result, they display different performance in food production and final food properties like consistency and taste. To address this issue, a fast, cheap, and simple method for screening and characterization is required. In this article, the successful analysis of soy protein isolates, a complex mixture of proteins with glycinin and beta-conglycinin as major components, by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is demonstrated. The preparation implements a fast extraction of the proteins from the raw SPI either under neutral or reducing conditions. The extracts are analyzed subsequently by MALDI-TOF-MS without further purification. Results of the two conditions are compared. Finally, different SPIs from different suppliers are analyzed and compared concerning their consistency. The method could be applied to other plant proteins and mixtures thereof. Since the composition and intactness of different subunits play important roles in functional properties of soy proteins, rapid methods for fingerprinting of different industrial soy protein sources will be valuable tools for successful product formulation.
Horneffer
Verena
V
Unilever Food and Health Research Institute, Unilever Research and Development Vlaardingen, The Netherlands. horneffer@mll.mu-luebeck.de
Foster
Tim J
TJ
Velikov
Krassimir P
KP
D052179:Antigens, Plant; D005916:Globulins / Q000032:analysis / Q000737:chemistry; D055314:Seed Storage Proteins; D012680:Sensitivity and Specificity; D030262:Soybean Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D052179:Antigens, Plant; D005916:Globulins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; C011474:glycinin