Oral administration of attenuated S. typhimurium carrying shRNA-expressing vectors as a cancer therapeutic.
RNAi has been successfully applied in genomic research, and it also holds considerable promise as a therapeutic approach to suppress disease-causing gene expression. Here, we show that attenuated S. typhimurium were capable of delivering shRNA-expressing vectors to mammalian cells and inducing RNAi in vitro and in vivo. Upon oral administration, S. typhimurium carrying shRNA-expressing vectors targeting bcl2 induced significant gene silencing in murine melanoma cells that led to a remarkably delayed tumor growth and prolonged survival in the mouse model. These results suggest that bacteria mediated RNAi may be a new potent approach to the treatment of cancers.
Yang
Nan
N
Institute of Genetics, Fudan University, Shanghai, PR China.
Zhu
Xiangying
X
Chen
Lishan
L
Li
Shenghua
S
Ren
Daming
D
D000284:Administration, Oral; D000818:Animals; D015536:Down-Regulation; D018014:Gene Transfer Techniques; D019254:Genes, bcl-2; D005822:Genetic Vectors; D051379:Mice; D009369:Neoplasms / Q000628:therapy*; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics*
D034741:RNA, Small Interfering