Gait analysis in a murine model of collagen-induced arthritis.
Murine collagen-induced arthritis (CIA) has become a valuable animal model for elucidating pathogenic mechanisms and evaluating therapeutic effects for rheumatoid arthritis. Recent advances in digital imaging and computer technology have enabled gait analysis to develop into a powerful tool for objectively detecting functional deficits in human and animal models. The present study explored the use of non-invasive video-capture gait analysis in the evaluation of a murine CIA model. CIA was induced in 45 female DBA/1LacJ mice (8 to 10 weeks old) by immunization with lyophilized bovine articular type II collagen. Gait parameters were determined by ventral plane videography and were correlated to traditional arthritis clinical scores. Our results showed that increases in clinical scores that measure the severity of CIA corresponded to changes in multiple gait parameters that reflect both morphologic (increases in paw area) and functional (increase in stride frequency, decrease in stride length, hind-limb paw placement angle, as well as stride, stance, and braking times) deficits. Our work indicated that the non-invasive video-capture device may be used as a simple and objective data acquisition system for quantifying gait disturbances in CIA mice for the investigation of mechanisms and the evaluation of therapeutic agents.
Bayer HealthCare Pharmaceuticals, 800 Dwight Way, Berkeley, CA 94701, USA. jonvincel@yahoo.com
Jon
J
Vincelette
Yifan
Y
Xu
Le-Ning
LN
Zhang
Caralee J
CJ
Schaefer
Ronald
R
Vergona
Mark E
ME
Sullivan
Thomas G
TG
Hampton
Yi-Xin Jim
YX
Wang
D000818:Animals; D001169:Arthritis, Experimental / Q000150:complications / Q000503:physiopathology*; D001172:Arthritis, Rheumatoid / Q000503:physiopathology; D001696:Biomechanical Phenomena; D005260:Female; D005684:Gait / Q000502:physiology*; D006801:Humans; D007794:Lameness, Animal / Q000209:etiology / Q000503:physiopathology*; D051379:Mice; D008811:Mice, Inbred DBA; D013997:Time Factors; D014741:Video Recording