The BaeSR two-component regulatory system mediates resistance to condensed tannins in Escherichia coli.
The gene expression profiles of Escherichia coli strains grown anaerobically with or without Acacia mearnsii (black wattle) extract were compared to identify tannin resistance strategies. The cell envelope stress protein gene spy and the multidrug transporter-encoding operon mdtABCD, both under the control of the BaeSR two-component regulatory system, were significantly up-regulated in the presence of tannins. BaeSR mutants were more tannin sensitive than their wild-type counterparts.
Zoetendal
Erwin G
EG
Department of Animal Sciences and Division of Nutritional Sciences, Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
Smith
Alexandra H
AH
Sundset
Monica A
MA
Mackie
Roderick I
RI
D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D020411:Oligonucleotide Array Sequence Analysis; D009876:Operon; D033903:Periplasmic Proteins / Q000235:genetics / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013634:Tannins / Q000494:pharmacology*
D029968:Escherichia coli Proteins; D033903:Periplasmic Proteins; C104888:Spy protein, E coli; D013634:Tannins