Variable number of tandem repeats and pulsed-field gel electrophoresis cluster analysis of enterohemorrhagic Escherichia coli serovar O157 strains.
Ninety-five enterohemorrhagic Escherichia coli serovar O157 strains, including 30 strains isolated from 13 intrafamily outbreaks and 14 strains isolated from 3 mass outbreaks, were studied by pulsed-field gel electrophoresis (PFGE) and variable number of tandem repeats (VNTR) typing, and the resulting data were subjected to cluster analysis. Cluster analysis of the VNTR typing data revealed that 57 (60.0%) of 95 strains, including all epidemiologically linked strains, formed clusters with at least 95% similarity. Cluster analysis of the PFGE patterns revealed that 67 (70.5%) of 95 strains, including all but 1 of the epidemiologically linked strains, formed clusters with 90% similarity. The number of epidemiologically unlinked strains forming clusters was significantly less by VNTR cluster analysis than by PFGE cluster analysis. The congruence value between PFGE and VNTR cluster analysis was low and did not show an obvious correlation. With two-step cluster analysis, the number of clustered epidemiologically unlinked strains by PFGE cluster analysis that were divided by subsequent VNTR cluster analysis was significantly higher than the number by VNTR cluster analysis that were divided by subsequent PFGE cluster analysis. These results indicate that VNTR cluster analysis is more efficient than PFGE cluster analysis as an epidemiological tool to trace the transmission of enterohemorrhagic E. coli O157.
Division of Bacteriology, Chiba Prefectural Institute of Public Health, 666-2, Chuo, Chiba City, Chiba 260-8715, Japan. e.ykym@ma.pref.chiba.lg.jp
Eiji
E
Yokoyama
Masako
M
Uchimura
D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000032:analysis*; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000635:transmission; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D005516:Food Microbiology; D005787:Gene Frequency; D006801:Humans; D007564:Japan; D018598:Minisatellite Repeats; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D022603:Shiga Toxins / Q000096:biosynthesis*; D020080:Tandem Repeat Sequences
D004269:DNA, Bacterial; D022603:Shiga Toxins