[Studies on cutaneous permeation in vitro of Kushen recipe gel].
OBJECTIVE
To establish a suitable dosage form for a traditional anti-anaphylaxis Chinese medicine of Kushen recipe, and investigate the effect of cutaneous permeation in vitro of the recipe.


METHODS
Techniques of extracting with ethanol and purifying with absorbent resin to obtain alkaloids from Kushen recipe were adopted, while volatile oil was extracted by steam distillation. The extraction was made to gel. The skin from SD rats' abdomen was used as permeability barriers. Then effects of permeation of the aqueous extraction, the purifying extraction and the gel were compared by Valia-Chien and Franz diffusion cell method. HPLC was utilized to quantitate the alkaloids in permeating liquid.


RESULTS
In view of the permeation cumulation quantity, the permeation velocity and the lag time of the four kinds of alkaloids, the effect of permeation of purifying extraction was better than the aqueous extraction, and the purifying extraction gel surpassed both the aqueous extraction and the purifying extraction.


CONCLUSIONS
It was certified that the purifying extraction gel had improved the effect of cutaneous permeation of alkaloids, and it is the befitting dosage form for Kushen recipe to treat anaphylaxis disease in skin.
Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Cheng
C
Peng
Jin-Hong
JH
Hu
Quan-Gang
QG
Zhu
Ji-Yong
JY
Liu
Zhuo
Z
Qin
Jing
J
Wang
D000279:Administration, Cutaneous; D000470:Alkaloids / Q000008:administration & dosage / Q000302:isolation & purification / Q000493:pharmacokinetics*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005260:Female; D005782:Gels; D066298:In Vitro Techniques; D011807:Quinolizines / Q000008:administration & dosage / Q000302:isolation & purification / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D000093842:Matrines
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D005782:Gels; D011807:Quinolizines; C046246:oxysophocarpine; C035933:sophocarpine; C037573:oxymatrine; C494416:kushen; D000093842:Matrines