Phonophoresis and the absorption of dexamethasone in the presence of an occlusive dressing.
BACKGROUND
Phonophoresis is purported to represent a method to apply topical medications through the skin to treat soft tissue injuries and inflammatory conditions. Few data are available to demonstrate the clinical effectiveness of the treatment.


OBJECTIVE
To determine the effect of ultrasound on the transcutaneous absorption of dexamethasone when occluded with a dressing.


METHODS
Crossover design.


METHODS
University general clinical research center.


METHODS
Ten healthy subjects (age = 29.2 +/- 8.8 years; height = 170.0 +/- 3.9 cm; mass = 67.5 +/- 18.4 kg).


METHODS
Two grams of 0.33% dexamethasone cream were applied to a 10-cm (2) area on the anterior forearm. The drug was applied to the skin and occluded with a dressing for 30 minutes before the ultrasound and sham ultrasound treatments. The treatments were applied over the drug and occlusive dressing. Ultrasound treatments were delivered at an intensity of 1.0 W/cm (2) (50% pulsed) at an output frequency of 3 MHz for 5 minutes and compared with sham ultrasound treatments that were delivered at an intensity of 0.0 W/cm (2) (50% pulsed) at an output frequency of 3 MHz for 5 minutes. All subjects received both the ultrasound and sham treatments, and the order in which subjects received the treatments was counterbalanced.


METHODS
Serum samples were drawn before treatment and immediately posttreatment and at 2, 4, 6, 8, and 10 hours posttreatment. Using high-performance liquid chromatography, we analyzed serum to determine dexamethasone concentrations.


RESULTS
A 2-way repeated-measures analysis of variance (condition x time) revealed a significant main effect for ultrasound treatment ( P = .047). The rate of appearance and the total concentration of dexamethasone in the serum were greater in subjects after phonophoresis than after sham ultrasound. The sham group had only trace amounts of dexamethasone in the serum, indicating that drug absorption was negligible without the ultrasound energy. The effect size of the phonophoresis condition fell within a 95% confidence interval after the baseline measurement.


CONCLUSIONS
We found that a phonophoretic effect occurred with dexamethasone when its application saturated the skin.
Athletic Training & Sports Medicine, University of Virginia, 210 Emmet Street South, PO Box 400407, Charlottesville, VA 22904-4269, USA. Saf8u@virginia.edu
Susan
S
Saliba
Dilaawar J
DJ
Mistry
David H
DH
Perrin
Joe
J
Gieck
Arthur
A
Weltman
D000279:Administration, Cutaneous; D000328:Adult; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D018592:Cross-Over Studies; D003907:Dexamethasone / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008297:Male; D009779:Occlusive Dressings; D015612:Phonophoresis; D012869:Skin Absorption; D016896:Treatment Outcome
D000893:Anti-Inflammatory Agents; D003907:Dexamethasone