Drosophila UTX is a histone H3 Lys27 demethylase that colocalizes with the elongating form of RNA polymerase II.
Histone H3 methylation at Lys27 (H3K27 methylation) is a hallmark of silent chromatin, while H3K4 methylation is associated with active chromatin regions. Here we report that a Drosophila JmjC family member, dUTX, specifically demethylates di- and trimethylated but not monomethylated H3K27. dUTX localization on chromatin correlates with the elongating form of RNA polymerase II (Pol II), and dUTX can associate with Pol II. Furthermore, heat shock induction results in the recruitment of dUTX to the hsp70 gene, like that of several other Pol II elongation factors. Our data indicate that dUTX is intimately associated with actively transcribed genes and may provide a paradigm for how H3K27 demethylation is required for the activation of preinitiated Pol II on transcriptionally poised genes.
Smith
Edwin R
ER
Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA.
Lee
Min Gyu
MG
Winter
Benjamin
B
Droz
Nathan M
NM
Eissenberg
Joel C
JC
Shiekhattar
Ramin
R
Shilatifard
Ali
A
D000818:Animals; D002843:Chromatin / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006657:Histones / Q000378:metabolism; D008297:Male; D008745:Methylation; D009687:Nuclear Proteins / Q000378:metabolism*; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism*; D014158:Transcription, Genetic
D002843:Chromatin; D029721:Drosophila Proteins; D018840:HSP70 Heat-Shock Proteins; D006657:Histones; D009687:Nuclear Proteins; D010089:Oxidoreductases, N-Demethylating; C527283:UTX protein, Drosophila; D012319:RNA Polymerase II