[Development of amplification technology-based methods for tick-borne encephalitis virus detection and genotyping].
The development of methods for tick-borne encephalitis (TBE) virus genotyping, such as determination of nucleotide sequences, analysis of restriction fragment length polymorphism, polymerase chain reaction using genotype-specific probes with hybridization-fluorescence detection, is under consideration. The capacities and application area of varions typing techniques, such as TBE detection and genotyping in the field, autoptic, and cultural materials; study of circulating genotypes in different natural foci and in various viral vectors and carriers, are described. Each technique was tested using a large number of strains isolated from ill humans and ticks in different regions of the country and employed to investigate abundant materials.
Karan'
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Braslavskaia
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Miazin
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D000818:Animals; D001096:Arachnid Vectors / Q000821:virology; D001483:Base Sequence; D004197:Disease Reservoirs / Q000821:virology; D004669:Encephalitis Viruses, Tick-Borne / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004675:Encephalitis, Tick-Borne / Q000175:diagnosis / Q000517:prevention & control; D005453:Fluorescence; D005814:Genes, Viral / Q000235:genetics; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D013987:Ticks / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics
D015345:Oligonucleotide Probes; D014759:Viral Envelope Proteins; C065696:glycoprotein E, Flavivirus