Desulfotignum toluenicum sp. nov., a novel toluene-degrading, sulphate-reducing bacterium isolated from an oil-reservoir model column.
A Gram-negative, sulphate-reducing bacterium (strain H3(T)) was isolated from an oil-reservoir model column. The new isolate was able to oxidize toluene coupled to hydrogen sulphide production. For growth, the optimum salt concentration was 1.5 % (w/v), the optimum pH was 7.2 and the optimum temperature was 34 degrees C. The cells were straight to slightly curved rods, 0.6-1.0 microm in diameter and 1.4-2.5 microm in length. The predominant fatty acids were C(16 : 0), C(16 : 1)omega7c and C(17 : 0) cyclo, and the cells also contained dimethylacetals. Cloning and sequencing of a 1505 bp long fragment of the 16S rRNA gene showed that strain H3(T) is a member of the Deltaproteobacteria and is related closely to Desulfotignum balticum DSM 7044(T). The G+C content of the DNA was 52.0 mol% and the DNA-DNA similarity to D. balticum DSM 7044(T) was 56.1 %. Based on differences in DNA sequence and the unique property of toluene degradation, it is proposed that strain H3(T) should be designated a member of a novel species within the genus Desulfotignum, for which the name Desulfotignum toluenicum sp. nov. is proposed. The type strain is H3(T) (=DSM 18732(T)=ATCC BAA-1460(T)).
Ommedal
Hege
H
University of Bergen, Department of Biology, Jahnebakken 5, N-5020 Bergen, Norway.|University of Bergen, Centre for Integrated Petroleum Research (CIPR), Allégaten 41, N-5007 Bergen, Norway.
Torsvik
Terje
T
University of Bergen, Centre for Integrated Petroleum Research (CIPR), Allégaten 41, N-5007 Bergen, Norway.
D000080:Acetals / Q000032:analysis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020564:Deltaproteobacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D006862:Hydrogen Sulfide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010578:Petroleum / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism*; D013696:Temperature; D014050:Toluene / Q000378:metabolism*
D000080:Acetals; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D014050:Toluene; D012965:Sodium Chloride; D006862:Hydrogen Sulfide; C059033:1,1-dimethoxyethane