Expression of glucagon-like peptide-1 in the taste buds of rat circumvallate papillae.
Glucagon-like peptide-1 (GLP-1), a 30-amino-acid peptide hormone, is a typical peptide of the brain-gut axis and can affect the metabolism of various tissues and organs. GLP-1 is secreted by intestinal L cells in response to nutrient ingestion. Some studies have shown that taste signaling elements were co-expressed in enteroendocrine cells of the small intestine, and in particular by L-cells. The present study was performed to explore the protein and mRNA expression of GLP-1 in the taste buds of rat circumvallate papillae and to try to determine the significance of its secretion. GLP-1 immunoreactivity was observed in spindle-shaped taste bud cells, with positive cells displaying a characteristic distribution of reaction product that was confined to the cytosol. Reverse transcription polymerase chain reaction (RT-PCR) assay showed that GLP-1 mRNA was expressed in circumvallate papillae. The expression of GLP-1 suggests that it may play an important role in the taste stimulation of nutrients and gut hormone secretion.
Department of Endocrinology, The First Clinical Hospital of Harbin Medical University, Harbin 150001, China.
Xiao-Hong
XH
Feng
Xiao-Min
XM
Liu
Li-Hong
LH
Zhou
Jing
J
Wang
Guo-Dong
GD
Liu
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015870:Gene Expression; D020869:Gene Expression Profiling; D052216:Glucagon-Like Peptide 1 / Q000032:analysis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013650:Taste Buds / Q000378:metabolism*
D000906:Antibodies; D052216:Glucagon-Like Peptide 1