Peroxisome proliferation in Hansenula polymorpha requires Dnm1p which mediates fission but not de novo formation.
We show that the dynamin-like proteins Dnm1p and Vps1p are not required for re-introduction of peroxisomes in Hansenula polymorpha pex3 cells upon complementation with PEX3-GFP. Instead, Dnm1p, but not Vps1p, plays a crucial role in organelle proliferation via fission. In H. polymorpha DNM1 deletion cells (dnm1) a single peroxisome is present that forms long extensions, which protrude into developing buds and divide during cytokinesis. Budding pex11.dnm1 double deletion cells lack these peroxisomal extensions, suggesting that the peroxisomal membrane protein Pex11p is required for their formation. Life cell imaging revealed that fluorescent Dnm1p-GFP spots fluctuate between peroxisomes and mitochondria. On the other hand Pex11p is present over the entire organelle surface, but concentrates during fission at the basis of the organelle extension in dnm1 cells. Our data indicate that peroxisome fission is the major pathway for peroxisome multiplication in H. polymorpha.
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Haren, The Netherlands.
Shirisha
S
Nagotu
Ruchi
R
Saraya
Marleen
M
Otzen
Marten
M
Veenhuis
Ida J
IJ
van der Klei
D034281:Dynamins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D008565:Membrane Proteins / Q000502:physiology; D008928:Mitochondria / Q000737:chemistry; D020675:Peroxisomes / Q000737:chemistry / Q000648:ultrastructure*; D010843:Pichia / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure*
D005656:Fungal Proteins; D008565:Membrane Proteins; D034281:Dynamins