Determination of water-soluble polyphenolic compounds in commercial herbal teas from Lamiaceae: peppermint, melissa, and sage.
Chromatographic techniques (HPLC and HPTLC) were used for qualitative and quantitative determination of eriocitrin, luteolin 7-O-rutinoside, luteolin 7-O-beta-glucuronide, lithospermic acid, rosmarinic acid, and methyl rosmarinate together with other known compounds in commercial herbal teas from the Lamiaceae family: peppermint leaf (Menthae piperitae folium), melissa leaf (Melissae folium), and sage leaf (Salviae officinalis folium). Contents of analyzed compounds in infusions, the most popular forms, were established using a C18 column with acetonitrile-water-formic acid as a mobile phase. The HPLC method was validated for linearity, precision, and accuracy. Luteolin 7-O-beta-glucuronide and lithospermic acid were identified as new Mentha x piperita compounds. The investigated herbal teas delivered polyphenols in high amounts, up to 182.2 mg for the infusion of one peppermint tea bag.
Fecka
Izabela
I
Department of Pharmacognosy, Wrocław Medical University, Poland. izabela@farmgn.am.wroc.pl
Turek
Sebastian
S
D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D031334:Melissa / Q000737:chemistry*; D036142:Mentha piperita / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols; D027543:Salvia officinalis / Q000737:chemistry*; D012995:Solubility; D014867:Water
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D014867:Water