OligoPrep PVA support for oligonucleotide synthesis in columns on a scale up to 10 micromol.
OligoPrep is a macroporous polyvinylacetate (PVA) biodegradable support that has been designed for cost-effective automated synthesis of oligonucleotides using standard phosphoramidite chemistry. Originally developed for large-scale oligonucleotide synthesis in beds and reactors, we present here its utility for medium-scale work of 1-10 micromol in column syntheses on standard DNA synthesizers. We show how an increase in scale, and, therefore, yield, can be achieved without significant increase in reagent quantity. Additional deblock and oxidation cycles can provide high coupling yields, and the use of concentrated ammonia in aqueous methylamine (AMA) for oligonucleotide cleavage and deprotection results in excellent recovery.
Link Technologies Ltd., Strathclyde Business Park, Bellshill, Scotland.
Sheena
S
Aitken
Emma
E
Anderson
D000641:Ammonia; D002851:Chromatography, High Pressure Liquid; D008744:Methylamines; D008832:Microchemistry / Q000379:methods; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010084:Oxidation-Reduction; D011145:Polyvinyls
D008744:Methylamines; D009841:Oligonucleotides; D011145:Polyvinyls; C013215:polyvinyl acetate; D000641:Ammonia; C027451:methylamine