A novel biotinylated diazirinyl ceramide analogue for photoaffinity labeling.
A novel photoreactive ceramide analogue, which contains (3-trifluoromethyl)phenyldiazirinyl lipid and biotinylated sphingosine, was synthesized. The probe was recognized as an antigen by anti-ceramide antibody and as a substrate for sphingolipid ceramide N-deacylase. These results indicate that the probe may be useful as a photoaffinity-biotinylating agent in sphingolipid studies.
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Hokkaido, Japan. hasimoto@obihiro.ac.jp
Makoto
M
Hashimoto
Yasumaru
Y
Hatanaka
D001710:Biotin / Q000737:chemistry*; D002518:Ceramides / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D049449:Luminescence; D019920:Photoaffinity Labels
D002518:Ceramides; D019920:Photoaffinity Labels; D001710:Biotin