Synthesis of photoactive analogues of a cystine knot trypsin inhibitor protein.
We describe the preparation of a recently described photoactive amino acid analogue (photoMethionine) by two novel synthetic routes, one of which is flexible and enantiospecific, and the site-specific chemical incorporation of photoMethionine into a defined and functionally active protein using a combination of solid-phase peptide synthesis and modern chemical ligation methodology. Site-specific labeling of proteins with this amino acid analogue through chemical synthesis provides valuable probes for photoaffinity cross-linking studies.
Durek
Thomas
T
Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois 60637, USA.
Zhang
Junliang
J
He
Chuan
C
Kent
Stephen B H
SB
D002853:Chromatography, Liquid; D047168:Cystine Knot Motifs; D021241:Spectrometry, Mass, Electrospray Ionization; D014361:Trypsin Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry
D014361:Trypsin Inhibitors