Evaluation of immunochromatographic assay for serodiagnosis of Brucella canis.
Canine brucellosis is a contagious disease with venereal and oral modes of transmission that produces late abortion in females, epididymides and prostates in males. Diagnosis is difficult because of unstable serum antibody titers that vary from individual to individual as well as between different methods used for their detection. The objective of this work was to evaluate the clinical utility of the immunochromatographic assay (ICA) for serodiagnosis of dogs suspected of having brucellosis, and results were compared with those obtained for hemoculture (HC) and the rapid screening agglutination with 2-mercaptoethanol (2-ME RSAT). The all experimentally infected dogs were positive in ICA, HC and 2-ME RSAT from 5 weeks, 7 weeks, and 3 weeks after infection, respectively. Also, among dogs selected from 10 different breed kennels occurred brucellosis, 24.8%, 39.5% and 39.1% of dogs tested were detected as positive with HC, 2-ME RSAT and ICA, respectively. The kappa value between 2-ME RSAT and ICA was 0.89. The results of this study showed that sensitivity and specificity of the ICA are comparable with those obtained using conventional serological and bacteriological test for brucellosis. In conclusion, the ICA kit provides a handy and accurate tool for the rapid serodiagnosis of canine brucellosis.
Kim
Jong Wan
JW
National Veterinary Research and Quarantine Service, Anyang, Korea.
Lee
Young Ju
YJ
Han
Moo Yeol
MY
Bae
Dong Hwa
DH
Jung
Suk Chan
SC
Oh
Jin Sik
JS
Ha
Gun Woo
GW
Cho
Byung Ki
BK
D000818:Animals; D041002:Brucella canis / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D002845:Chromatography / Q000379:methods*; D004283:Dog Diseases / Q000175:diagnosis* / Q000382:microbiology*; D004285:Dogs; D007118:Immunoassay / Q000379:methods*; D012698:Serologic Tests