Cholic acid functionalized star poly(DL-lactide) for promoting cell adhesion and proliferation.
Cholic acid functionalized star poly(DL-lactide) was synthesized through the ring-opening polymerization of DL-lactide initiated by cholic acid. The properties and cell behaviour of the cholic acid functionalized star poly(DL-lactide) were investigated as compared with linear poly(DL-lactide)s with different molecular weights and a star poly(DL-lactide) initiated by glycerol. In comparison to linear poly(DL-lactide)s, the cholic acid functionalized star poly(DL-lactide) had better wettability and slightly higher surface energy. The cell adhesion and proliferation on different materials were evaluated using two types of cells, 3T3 mouse fibroblasts and ECV304 human endothelial cells. Compared with the linear poly(DL-lactide)s, the cholic acid functionalized star poly(DL-lactide) showed obviously improved property for cell adhesion. The cell proliferation on the cholic acid functionalized star poly(DL-lactide) was also enhanced. The improvement in cell proliferation was not so significant as compared with the improvement in cell adhesion. This modification strategy provides an effective and simple way to promote cell attachment and growth in tissue engineering.
Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of China.
Hui-Li
HL
Fu
Tao
T
Zou
Si-Xue
SX
Cheng
Xian-Zheng
XZ
Zhang
Ren-Xi
RX
Zhuo
D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D040761:Biomimetic Materials / Q000737:chemistry; D002448:Cell Adhesion; D049109:Cell Proliferation; D019826:Cholic Acid / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D051379:Mice; D015394:Molecular Structure; D008970:Molecular Weight; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry; D017155:Wettability
D001672:Biocompatible Materials; D011091:Polyesters; C033616:poly(lactide); D019826:Cholic Acid