An enhanced miniaturized assay for antimicrobial prospecting.
Bioprospecting for novel antimicrobials increasingly relies on extremely small samples unsuitable for conventional bulk extraction and assay. We developed a microtitre plate assay for minimal amounts of test materials which is rapid, extremely sensitive, allows time-course analysis and reduces false negatives. Developed for the analyses of antimicrobial sensitivity and resistance, the technique is appropriate for assays where source materials are scarce.
Smith
Shannon M
SM
Department of Biological Sciences, Macquarie University, NSW, Australia. smsmith@bio.mq.edu.au
Beattie
Andrew J
AJ
Gillings
Michael R
MR
Holley
Marita P
MP
Stow
Adam J
AJ
Turnbull
Christine L
CL
Wilson
Peter D
PD
Briscoe
David A
DA
D000667:Ampicillin / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D020128:Inhibitory Concentration 50; D007313:Insecta / Q000737:chemistry; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010957:Plasmids; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013307:Streptomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D000667:Ampicillin; D013307:Streptomycin