Enumeration of haemagglutinin-specific CD8+ T cells after influenza vaccination using MHC class I peptide tetramers.
With emergence of MHC class I tetramers loaded with CD8+ T-cell viral epitopes, it is possible to study virus-specific CD8 cells in humans during infection and after vaccination. MHC class I tetramers was used to detect the frequency of haemagglutinin (HA)-specific T cells in 26 healthy influenza-vaccinated humans. Peripheral blood was collected before, and 7, 14 and 28 days after vaccination. Four-colour flow cytometry was used for monitoring of vaccine induced T-cell response. In 15 donors, two- to fivefold increase in frequency of HA-specific T cells was observed 7 days after vaccination. In addition, in 12 of these donors, this increase was accompanied with fourfold increase of H1N1 antibody titre. The increase in frequency of HA-specific CD8+/IFN-gamma+ cells was low and peaked 28 days after vaccination in three of the six donors tested. Frequencies of HA-specific CD8+ T cells and antibody titre returned to prevaccination values 1 year after vaccination. Subunit influenza vaccines have the ability to induce HA-specific CD8+ cells. As the immune response to this vaccine decreased significantly after 1 year, our results confirm the importance of annual immunization for adequate protection.
Institute of Immunology, Department of Research and Development, Cellular Immunology Unit, Zagreb, Croatia.
E
E
Kosor Krnic
A
A
Gagro
V
V
Drazenovic
I
I
Kuzman
T
T
Jeren
E
E
Cecuk-Jelicic
V
V
Kerhin-Brkljacic
I
I
Gjenero-Margan
B
B
Kaic
S
S
Rakusic
A
A
Sabioncello
A
A
Markotic
S
S
Rabatic
G
G
Mlinaric-Galinovic
D
D
Dekaris
D000328:Adult; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000821:virology*; D015234:HLA-A Antigens / Q000276:immunology*; D015789:HLA-A2 Antigen; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D018655:Lymphocyte Count; D008875:Middle Aged; D009439:Neuraminidase / Q000008:administration & dosage / Q000276:immunology; D010455:Peptides / Q000276:immunology*; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology
D015234:HLA-A Antigens; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D010455:Peptides; D022223:Vaccines, Subunit; D009439:Neuraminidase