Characterization of a late gene, ORF67 from Bombyx mori nucleopolyhedrovirus.
Open reading frame 67 of Bombyx mori nucleopolyhedrovirus (BmORF67) is a homologue of Autographa californica multiple NPV ORF81. The gene is conserved among all baculoviruses and is thus considered a baculovirus core gene. The transcript of BmORF67 was detected at 18-72 h post-infection (p.i.). Polyclonal antiserum raised to a His-BmORF67 fusion protein recognized BmORF67 in infected cell lysates from 24 to 72 h p.i., suggesting that BmORF67 is a late gene. BmORF67 was not detected either in budded viruses or occlusion-derived virus. Immunofluoresence analysis showed that the protein located in the cytoplasm and interacted with host protein actin A3. In conclusion, BmORF67 is a late protein localized in the cytoplasm of infected cells that interacts with host protein.
Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, PR China.
Hui-qing
HQ
Chen
Ke-ping
KP
Chen
Qin
Q
Yao
Zhong-jian
ZJ
Guo
Ling-ling
LL
Wang
D000199:Actins / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D012831:Bombyx / Q000166:cytology / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D000199:Actins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins