Elastic image registration of 2-D gels for differential and repeatability studies.
One of the main applications of electrophoretic 2-D gels is the analysis of differential responses between different conditions. For this reason, specific spots are present in one of the images, but not in the other. In some other occasions, the same experiment is repeated between 2 and 12 times in order to increase statistical significance. In both situations, one of the major difficulties of these analysis is that 2-D gels are affected by spatial distortions due to run-time differences and dye-front deformations, resulting in images that are significantly dissimilar not only because of their content, but also because of their geometry. In this technical brief, we show how to use free, state-of-the-art image registration and fusion algorithms developed by us for solving the problem of comparing differential expression profiles, or computing an "average" image from a series of virtually identical gels.
Sorzano
Carlos O S
CO
Bioengineering Laboratory, University San Pablo-CEU, Campus Urb. Monteprincipe s/n, Boadilla del Monte, Madrid, Spain. coss.eps@ceu.es
Arganda-Carreras
Ignacio
I
Thévenaz
Philippe
P
Beloso
Ana
A
Morales
Gracia
G
Valdés
Israel
I
Pérez-García
Carmen
C
Castillo
Carmen
C
Garrido
Elisa
E
Unser
Michael
M
D000465:Algorithms; D000818:Animals; D004548:Elasticity; D015180:Electrophoresis, Gel, Two-Dimensional; D007090:Image Interpretation, Computer-Assisted; D007091:Image Processing, Computer-Assisted; D010363:Pattern Recognition, Automated; D020543:Proteome / Q000737:chemistry; D016958:Pseudomonas putida / Q000737:chemistry; D051381:Rats; D013382:Subtraction Technique
D020543:Proteome