Proteome analysis of Persian sturgeon (Acipenser persicus) ova.
The Persian sturgeon ova are a key material both for inevitable artificial propagation and for caviar production. In this study, the proteome profile of Persian sturgeon ova was analyzed using 2-DE and MALDI-TOF/TOF in order to determine its protein composition. Out of 192 spots analyzed with MALDI-TOF/TOF, 107 spots corresponding to 73 different proteins were identified. The identified proteins were classified into 11 groups with regard to their main known function involving cell structure (24.65%), translation and transcription (12.32%), metabolism and energy production (12.32%), protein synthesis (9.60%), membrane protein receptors or cell signaling (8.21%), cell defense (5.47%), transport (5.47%), cell division (8.21%), vitellogenin (2.73%), unclassified (6.84%) and unknown function (4.10%). The results of this study provide a valuable resource for molecular analysis of normal and abnormal conditions affecting female reproduction. Moreover, it may help to better understand factors affecting caviar quality during refrigerated storage.
Department of Fisheries, College of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran.
Saeed
S
Keyvanshokooh
Behrouz
B
Vaziri
D000818:Animals; D004527:Egg Proteins / Q000737:chemistry / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism; D004798:Enzymes / Q000737:chemistry / Q000302:isolation & purification; D005260:Female; D005399:Fishes / Q000235:genetics*; D010060:Ovulation; D010063:Ovum / Q000502:physiology; D010449:Peptide Mapping; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D020543:Proteome / Q000235:genetics*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin; D014819:Vitellogenins / Q000737:chemistry / Q000302:isolation & purification
D004527:Egg Proteins; D004798:Enzymes; D011506:Proteins; D020543:Proteome; D014819:Vitellogenins; D014357:Trypsin