Adipogenesis of bovine perimuscular preadipocytes.
In this study, non-transformed progeny adipofibroblasts, derived from mature adipocyte dedifferentiation, was used as a novel in vitro model to study adipogenic gene expression in cattle. Adipofibroblasts from dedifferentiated mature perimuscular fat (PMF) tissue were cultured with differentiation stimulants until the cells exhibited morphological differentiation. Treated cells were harvested from day 2 to 16 for RNA extraction, whereas control cells were cultured without addition of stimulants. Results from time course gene expression assays by quantitative real-time PCR revealed that peroxisome proliferator-activated receptor gamma (PPAR-gamma), sterol regulatory element binding protein 1 (SREBP-1) and their six down-stream genes were co-expressed at day 2 post-differentiation induction. When compared to other adipogenesis culture systems, the adipogenic gene expression of bovine PMF adipofibroblasts culture was different, especially to the rodent model. Collectively, these results demonstrated PPAR-gamma and SREBP-1 cooperatively play a key role to regulate the re-differentiation of bovine adipofibroblasts, during early conversion stages in vitro.
Taniguchi
Masaaki
M
Department of Agricultural, Food and Nutritional Science, University of Alberta, 410 Ag-For Centre, Edmonton, Alta., Canada T6G 2P5.
Guan
Le Luo
LL
Zhang
Bing
B
Dodson
Michael V
MV
Okine
Erasmus
E
Moore
Stephen S
SS
D017667:Adipocytes / Q000166:cytology* / Q000502:physiology*; D050156:Adipogenesis / Q000502:physiology*; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D032342:Muscle Cells / Q000166:cytology* / Q000502:physiology*
D036341:Intercellular Signaling Peptides and Proteins