Characterization of CD34+ cells from canine umbilical cord blood, bone marrow leukocytes, and peripheral blood by flow cytometric analysis.
Characterization of CD34+ cells in canine bone marrow, umbilical cord blood, and peripheral blood was performed by flow cytometric analysis. The ratio of CD34+CD45hi cells, which are absent in human blood, was high in the CD34+ cell fraction, but 98% of these was suggested B-cells. The remaining CD34+CD45lo cells may comprise canine hematopoietic progenitor cells, and these cells accounted for 0.23 +/- 0.07% of the fraction in cord blood, 0.30 +/- 0.07% in bone marrow, and 0.02 +/- 0.01% in peripheral blood.
Laboratory of Therigenology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fukjsawa, Kanagawa 252-8510, Japan. tsumagar@brs.nihon-n.ac.jp
Shigehisa
S
Tsumagari
Isao
I
Otani
Koichi
K
Tanemura
Shinichi
S
Namba
Tadatoshi
T
Ohtaki
Hiroshi
H
Kamata
Kiichi
K
Kanayama
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000378:metabolism*; D004285:Dogs; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry / Q000662:veterinary*; D007962:Leukocytes / Q000378:metabolism*; D014470:Umbilical Cord
D018952:Antigens, CD34