Ectopic expression of apple MbR7 gene induced enhanced resistance to transgenic Arabidopsis plant against a virulent pathogen.
A disease resistance related gene, MbR7, was identified in the wild apple species, Malus baccata. The MbR7 gene has a single open reading frame (ORF) of 3,288 nucleotides potentially encoding a 1,095-amino acid protein. Its deduced amino acid sequence resembles the N protein of tobacco and the NL27 gene of potato and has several motifs characteristic of a TIR-NBS-LRR R gene subclass. Ectopic expression of MbR7 in Arabidopsis enhanced the resistance against a virulent pathogen, Pseudomonas syringae pv. tomato DC3000. Microarray analysis confirmed the induction of defense-related gene expression in 35S::MbR7 heterologous Arabidopsis plants, indicating that the MbR7 gene likely activates a downstream resistance pathway without interaction with pathogens. Our results suggest that MbR7 can be a potential target gene in developing a new disease-resistant apple variety.
Lee
Soo-Yeon
SY
Department of Life Science, Ewha Womans University, Seoul 120-750, Korea.
Choi
Yeon-Ju
YJ
Ha
Young-Mie
YM
Lee
Dong-Hee
DH
D017360:Arabidopsis / Q000235:genetics* / Q000382:microbiology*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D020869:Gene Expression Profiling; D017343:Genes, Plant; D027845:Malus / Q000235:genetics* / Q000382:microbiology*; D020411:Oligonucleotide Array Sequence Analysis; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000472:pathogenicity*; D014774:Virulence
D017931:DNA Primers; D018744:DNA, Plant