Secondary structure and distribution of fusogenic LV-peptides in lipid membranes.
LV-peptides were designed as membrane-spanning low-complexity model structures that mimic fusion protein transmembrane domains. These peptides harbor a hydrophobic core sequence that consists of helix-promoting and helix-destabilizing residues at different ratios. Previously, the fusogenicity of these peptides has been shown to increase with the conformational flexibility of their hydrophobic cores as determined in isotropic solution. Here, we examined the secondary structure, orientation, and distribution of LV-peptides in membranes. Our results reveal that the peptides are homogeneously distributed within the membranes of giant unilamellar liposomes and capable of fusing them. Increasing the valine content of the core up to the level of the beta-branched residue content of SNARE TMDs (approximately 50%) enhances fusogenicity while maintaining a largely alpha-helical structure in liposomal membranes. A further increase in valine content or introduction of a glycine/proline pair favors beta-sheet formation. In planar bilayers, the alpha-helices adopt oblique angles relative to the bilayer normal and the ratio of alpha-helix to beta-sheet responds more sensitively to valine content. We propose that the fusogenic conformation of LV-peptides is likely to correspond to a membrane-spanning alpha-helix. Beta-sheet formation in membranes may be considered a side-reaction whose extent reflects conformational flexibility of the core.
Ollesch
J
J
Lehrstuhl für Biophysik, Ruhr-Universität Bochum, Bochum, Germany.
Poschner
B C
BC
Nikolaus
J
J
Hofmann
M W
MW
Herrmann
A
A
Gerwert
K
K
Langosch
D
D
D000595:Amino Acid Sequence; D002499:Centrifugation, Density Gradient; D002942:Circular Dichroism; D004578:Electron Spin Resonance Spectroscopy; D008051:Lipid Bilayers / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012997:Solvents; D017550:Spectroscopy, Fourier Transform Infrared; D013395:Sucrose / Q000737:chemistry
D008051:Lipid Bilayers; D008055:Lipids; D008081:Liposomes; D010455:Peptides; D012997:Solvents; D013395:Sucrose