Shewanella haliotis sp. nov., isolated from the gut microflora of abalone, Haliotis discus hannai.
A motile, rod-shaped, pink-orange pigmented bacterium, designated strain DW01(T), was isolated from the gut microflora of abalone collected from the South Sea (Republic of Korea). Cells were Gram-negative, facultatively anaerobic, catalase- and oxidase-positive. The major fatty acids were iso-C(15 : 0) (17.7 %), C(16 : 0) (13.4 %), iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c (12.5 %) and C(17 : 1)omega8c (10.7 %). The DNA G+C content was 53.7 mol%. A phylogenetic tree based on the 16S rRNA gene sequences showed that strain DW01(T) forms a lineage of the genus Shewanella and is closely related to Shewanella algae ATCC 51192(T) (98.3 % sequence similarity) and to other members of the genus Shewanella (91.0-94.9 %). The phenotypic characteristics and DNA-DNA hybridization relatedness data indicate that strain DW01(T) should be distinguished from S. algae ATCC 51192(T). On the basis of the data presented in this study, strain DW01(T) represents a novel species, for which the name Shewanella haliotis sp. nov. is proposed. The type strain is DW01(T) (=KCTC 12896(T)=JCM 14758(T)).
Kim
Duwoon
D
Division of Food Science and Aqualife Medicine, Chonnam National University, Yeosu 550-749, Republic of Korea.
Baik
Keun Sik
KS
Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon 540-742, Republic of Korea.
Kim
Mi Sun
MS
Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon 540-742, Republic of Korea.
Jung
Bok-Mi
BM
Division of Food Science and Aqualife Medicine, Chonnam National University, Yeosu 550-749, Republic of Korea.
Shin
Tai-Sun
TS
Division of Food Science and Aqualife Medicine, Chonnam National University, Yeosu 550-749, Republic of Korea.
Chung
Gyu-Hwa
GH
Department of Biotechnology, Chonnam National University, Yeosu 550-749, Republic of Korea.
Rhee
Moon Soo
MS
Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-600, Republic of Korea.
Seong
Chi Nam
CN
Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon 540-742, Republic of Korea.
D000332:Aerobiosis / Q000502:physiology; D000693:Anaerobiosis / Q000502:physiology; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041981:Gastrointestinal Tract / Q000382:microbiology; D049851:Gastropoda / Q000382:microbiology*; D020459:Genes, rRNA; D007723:Korea; D008124:Locomotion / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D020592:Shewanella / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; D002374:Catalase