Bacillus kribbensis sp. nov., isolated from a soil sample in Jeju, Korea.
A Gram-positive, endospore-forming bacterium, designated strain BT080(T), was isolated from a soil sample in Jeju, Korea. Cells of the isolate were strictly aerobic rods that were motile by means of peritrichous flagella. The strain grew optimally at 30-33 degrees C and pH 5.5-6.5. Chemotaxonomic data (major isoprenoid quinone, MK-7; DNA G+C content, 43.3 mol%; major fatty acids, anteiso-C(15 : 0), iso-C(14 : 0), iso-C(16 : 0) and iso-C(15 : 0)) supported the affiliation of the isolate to the genus Bacillus. Comparative 16S rRNA gene sequence analyses showed that strain BT080(T) formed a distinct phyletic line within the genus Bacillus. The levels of 16S rRNA gene sequence similarity with respect to related Bacillus species were below 96.4 %. On the basis of physiological, biochemical and phylogenetic properties, strain BT080(T) represents a novel species within the genus Bacillus, for which the name Bacillus kribbensis sp. nov. is proposed. The type strain is BT080(T) (=KCTC 13934(T)=DSM 17871(T)).
Lim
Jee-Min
JM
Functional Metabolomics Research Center, KRIBB, Daejeon 305-806, Korea.
Jeon
Che Ok
CO
Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
Lee
Jung Ro
JR
Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
Park
Dong-Jin
DJ
Functional Metabolomics Research Center, KRIBB, Daejeon 305-806, Korea.
Kim
Chang-Jin
CJ
Functional Metabolomics Research Center, KRIBB, Daejeon 305-806, Korea.
D000332:Aerobiosis / Q000502:physiology; D001407:Bacillus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005407:Flagella; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008124:Locomotion / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000166:cytology; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S