Functional annotation of 19,841 Populus nigra full-length enriched cDNA clones.
BACKGROUND
Populus is one of favorable model plants because of its small genome. Structural genomics of Populus has reached a breakpoint as nucleotides of the entire genome have been determined. Reaching the post genome era, functional genomics of Populus is getting more important for well-comprehended plant science. Development of bioresorce serving functional genomics is making rapid progress. Huge efforts have achieved deposits of expressed sequence tags (ESTs) in various plant species consequently accelerating functional analysis of genes. ESTs from full-length cDNA clones are especially powerful for accurate molecular annotation. We promoted collection and annotation of the ESTs from Populus full-length enriched cDNA clones as part of functional genomics of tree species.


RESULTS
We have been collecting the full-length enriched cDNA of the female poplar (Populus nigra var. italica) for years. By sequencing P. nigra full-length (PnFL) cDNA libraries, we generated about 116,000 5'-end or 3'-end ESTs corresponding to 19,841 nonredundant PnFL clones. Population of PnFL cDNA clones represents 44% of the predicted genes in the Populus genome.


CONCLUSIONS
Our resource of P. nigra full-length enriched clones is expected to provide valuable tools to gain further insight into genome annotation and functional genomics in Populus.
Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute (FFPRI), 1 Matsunosato, Tsukuba, Ibaraki 305-8687 JAPAN. nanjo@affrc.go.jp
Tokihiko
T
Nanjo
Tetsuya
T
Sakurai
Yasushi
Y
Totoki
Atsushi
A
Toyoda
Mitsuru
M
Nishiguchi
Tomoyuki
T
Kado
Tomohiro
T
Igasaki
Norihiro
N
Futamura
Motoaki
M
Seki
Yoshiyuki
Y
Sakaki
Kazuo
K
Shinozaki
Kenji
K
Shinohara
D000465:Algorithms; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003001:Cloning, Molecular / Q000379:methods; D016000:Cluster Analysis; D018076:DNA, Complementary / Q000032:analysis* / Q000145:classification / Q000502:physiology*; D030541:Databases, Genetic; D015723:Gene Library; D017343:Genes, Plant / Q000502:physiology; D018745:Genome, Plant; D032107:Populus / Q000235:genetics*; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary