Microbial community profiling in cis- and trans-dichloroethene enrichment systems using denaturing gradient gel electrophoresis.
The effective and accurate assessment of the total microbial community diversity is one of the primary challenges in modem microbial ecology, especially for the detection and characterization of unculturable populations and populations with a low abundance. Accordingly, this study was undertaken to investigate the diversity of the microbial community during the biodegradation of cis- and trans-dichloroethenes in soil and wastewater enrichment cultures. Community profiling using PCR targeting the 16S rRNA gene and denaturing gradient gel electrophoresis (PCR-DGGE) revealed an alteration in the bacterial community profiles with time. Exposure to cis- and trans-dichloroethenes led to the disappearance of certain genospecies that were initially observed in the untreated samples. A cluster analysis of the bacterial DGGE community profiles at various sampling times during the degradation process indicated that the community profile became stable after day 10 of the enrichment. DNA sequencing and phylogenetic analysis of selected DGGE bands revealed that the genera Acinetobacter, Pseudomonas, Bacillus, Comamonas, and Arthrobacter, plus several other important uncultured bacterial phylotypes, dominated the enrichment cultures. Thus, the identified dominant phylotypes may play an important role in the degradation of cis- and trans-dichloroethenes.
Discipline of Microbiology, Faculty of Science and Agriculture, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban 4000, South Africa. adeolaniran@yahoo.com
Ademola O
AO
Olaniran
William H L
WH
Stafford
Don A
DA
Cowan
Dorsamy
D
Pillay
Balakrishna
B
Pillay
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D044822:Biodiversity; D016000:Cluster Analysis; D004000:Dichloroethylenes / Q000378:metabolism*; D004586:Electrophoresis; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S; D012988:Soil Microbiology; D014871:Water Microbiology
D004000:Dichloroethylenes; D012336:RNA, Ribosomal, 16S