[Effect of phytohormones on plant regeneration in callus culture of Iris ensata Thunb].
The effect of phytohormones on plant regeneration in callus culture of Iris ensata Thunb. was studied. 2,4-Dichlorophenoxyacetic acid (2,4-D) proved to be the most convenient auxin for the induction and subculturing of morphogenic callus. Biogenic calluses were induced from isolated embryos at the wax-ripeness stage in MS medium supplemented with 2,4-D (1.0-2.0 mg/l) and kinetin (0.2-0.5 mg/l). Changes in the medium hormonal composition favor the development of adventitious structures. The setting of vegetative bugs took place in the presence of 6-benzylaminopurine, while the development of shoots and root primordia was observed after 2,4-D replacement with indoleacetic acid (2.0 mg/l). The root initiation in regenerating plants required a hormone-free medium. The properties of seedling growth from native regenerating I. ensata were studied. Analysis of the shoot structure demonstrated that in vitro cultured plants are at the juvenile stage.
Boltenkov
E V
EV
Mironova
L N
LN
Zarembo
E V
EV
D031323:Iris Plant / Q000166:cytology / Q000502:physiology*; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology*; D018517:Plant Roots / Q000166:cytology / Q000502:physiology*; D012038:Regeneration / Q000187:drug effects* / Q000502:physiology; D036226:Seedlings / Q000166:cytology / Q000502:physiology*
D010937:Plant Growth Regulators