Maximum diameter of the rod-shaped specimen for transmission electron microtomography without the "missing wedge".
In our recent study, the complete rotation of a rod-shaped specimen during transmission electron microscopy (TEM) has been successfully carried out, yielding a truly quantitative three-dimensional (3D) structure of a ZrO(2)/polymer nano-composite. This result allows the further development of transmission electron microtomography (TEMT) for materials science. The diameter of the rod-shaped specimen was about 150 nm, which may not be statistically large enough to evaluate structural parameters, e.g., volume fraction of Zr nano-particles. Thus, it is preferable to image rods with larger diameters in 3D. In this study, several rod-shaped specimens whose diameters ranged from 150 to 530 nm were subjected to the "distortion-free TEMT". The maximum diameters, l, observable under 200 and 300 kV-TEMTs were, respectively, 460-470 and 600-670 nm (corresponding the maximum relative diameters, l/lambda (lambda: mean free path), were ca. 2.2 and 2.7-3.0).
Department of Macromolecular Science and Engineering, Graduate School of Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Kyoto 606-8585, Japan.
Mitsuro
M
Kato
Noboru
N
Kawase
Takeshi
T
Kaneko
Shoichi
S
Toh
Syo
S
Matsumura
Hiroshi
H
Jinnai