Spirosoma rigui sp. nov., isolated from fresh water.
A Gram-negative, yellow-pigmented bacterium capable of gliding motility, designated strain WPCB118(T), was isolated from fresh water collected from the Woopo wetland (Republic of Korea). Cells were rod-shaped and sometimes filamentous. The major fatty acids were iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c (45.6 %), C(16 : 1)omega5c (18.5 %), iso-C(15 : 0) (9.5 %) and C(16 : 0) (8.8 %). The predominant menaquinone and polar lipid were MK-7 and phosphatidylethanolamine, respectively. The DNA G+C content was 53.3 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WPCB118(T) had an evolutionary lineage within the radiation encompassing the members of the family 'Flexibacteraceae', its closest neighbour being Spirosoma linguale LMG 10896(T) (93.7 % gene sequence similarity). Data from this polyphasic study indicated that strain WPCB118(T) could not be assigned to any recognized species. Strain WPCB118(T) represents a novel species of the genus Spirosoma, for which the name Spirosoma rigui sp. nov. is proposed. The type strain is WPCB118(T) (=KCTC 12531(T)=NBRC 101117(T)).
Baik
Keun Sik
KS
Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon 540-742, Republic of Korea.
Kim
Mi Sun
MS
Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon 540-742, Republic of Korea.
Park
Seong Chan
SC
Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon 540-742, Republic of Korea.
Lee
Dong Wan
DW
Department of Science Education, Cheju National University, Jeju 690-756, Republic of Korea.
Lee
Soon Dong
SD
Department of Science Education, Cheju National University, Jeju 690-756, Republic of Korea.
Ka
Jong-Ok
JO
Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea.
Choi
Sang Ki
SK
Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon 540-742, Republic of Korea.
Seong
Chi Nam
CN
Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon 540-742, Republic of Korea.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D007723:Korea; D008124:Locomotion / Q000502:physiology; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D024482:Vitamin K 2 / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2