Metagenomic analysis of BTEX-contaminated forest soil microcosm.
A microcosmal experiment using a metagenomic technique was designed to assess the effect of BTEX (benzene, toluene, ethylbenzene, and xylenes) on an indigenous bacterial community in a Daejeon forest soil. A compositional shift of bacterial groups in an artificial BTEX-contaminated soil was examined by the 16S rDNA PCR-DGGE method. Phylogenetic analysis of 16S rDNAs in the dominant DGGE bands showed that the number of Actinobacteria and Bacillus populations increased. To confirm these observations, we performed PCR to amplify the 23S rDNA and 16S rDNA against the sample metagenome using Actinobacteria-targeting and Bacilli-specific primer sets, respectively. The result further confirmed that a bacterial community containing Actinobacteria and Bacillus was affected by BTEX.
Ji
Sang Chun
SC
Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Korea.
Kim
Dockyu
D
Yoon
Jung Hoon
JH
Lee
Choong Hwan
CH
D039903:Actinobacteria / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D001407:Bacillus / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D001554:Benzene / Q000494:pharmacology*; D001555:Benzene Derivatives / Q000494:pharmacology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000494:pharmacology*; D014050:Toluene / Q000494:pharmacology*; D014197:Trees / Q000382:microbiology*; D014992:Xylenes / Q000494:pharmacology*
D001555:Benzene Derivatives; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D014992:Xylenes; D014050:Toluene; D001554:Benzene; C004912:ethylbenzene