Assembly of a three-dimensional array of glycoconjugates by combinatorial biocatalysis in nonaqueous media.
Glycoconjugates can be artificially synthesized by combinatorial biocatalysis. An example is given in this paper describing the construction of glycoconjugates array by using glycosidase and lipase in nonaqueous media. This array was started from glucose, with three aryl alcohols as the aglycone moiety of glycosides and five acids or esters as acyl donors for combinatorial acylation of glycosides, affording a three-dimensional array containing about 30 members with diverse structures. The array would be more abundant if more aglycones and acyl donors with other structures were filled in. Indeed, diverse classes of carbohydrates besides glucose can also be employed for generating diverse glycoconjugates due to their different roles in numerous physiological responses. The composition and distribution of the demonstration glycoconjugates array was detected and evaluated by HPLC-MS with electrospray ionization. And also, the distribution of the artificial array can be adjusted by changing the molar ratio of the auxiliary materials.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, P.R. China. jianhexu@ecust.edu.cn
Hui-Lei
HL
Yu
Jian-He
JH
Xu
Yu-Xiao
YX
Wang
Wen-Ya
WY
Lu
Guo-Qiang
GQ
Lin
D000215:Acylation; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D006001:Glycoconjugates / Q000138:chemical synthesis* / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure
D006001:Glycoconjugates; D006027:Glycosides; D008049:Lipase; D006026:Glycoside Hydrolases