Subunit-selective role of the M3 transmembrane domain of the nicotinic acetylcholine receptor in channel gating.
The nicotinic acetylcholine receptor (AChR) can be either hetero-pentameric, composed of alpha and non-alpha subunits, or homo-pentameric, composed of alpha7 subunits. To explore the subunit-selective contributions of transmembrane domains to channel gating we analyzed single-channel activity of chimeric muscle AChRs. We exchanged M3 between alpha1 and epsilon or alpha7 subunits. The replacement of M3 in alpha1 by epsilonM3 significantly alters activation properties. Channel activity appears as bursts of openings whose durations are 20-fold longer than those of wild-type AChRs. In contrast, 7-fold briefer openings are observed in AChRs containing the reverse epsilon chimeric subunit. The duration of the open state decreases with the increase in the number of alpha1M3 segments, indicating additive contributions of M3 of all subunits to channel closing. Each alpha1M3 segment decreases the energy barrier of the closing process by approximately 0.8 kcal/mol. Partial chimeric subunits show that small stretches of the M3 segment contribute additively to the open duration. The replacement of alpha1 sequence by alpha7 in M3 leads to 3-fold briefer openings whereas in M1 it leads to 10-fold prolonged openings, revealing that the subunit-selective role is unique to each transmembrane segment.
Instituto de Investigaciones Bioquímicas, Universidad Nacional del Sur-CONICET, Camino La Carrindanga Km 7, B8000FWB, Bahía Blanca, Argentina.
María José
MJ
De Rosa
Jeremías
J
Corradi
Cecilia
C
Bouzat
D000595:Amino Acid Sequence; D015640:Ion Channel Gating / Q000502:physiology*; D008969:Molecular Sequence Data; D018408:Patch-Clamp Techniques; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D013816:Thermodynamics
D011978:Receptors, Nicotinic