[Proteomics analysis of fat body from silkworm (Bombyx mori)].
High resolution two-dimensional polyacrylamide gel electrophoresis, followed by computer-assisted analysis, was applied to investigate the fat body of silkworm, Bombyx mori. 722 spots were obtained by silver stain from 18 cm, pH 3-10 gel. Most of them were distributed in the area from 15 kD to 90 kD with pH 4 - 8. The matrix-assisted laser desorption ionization time of flight mass spectrometry were applied for identifying the major spots of the 2D map. A total of 41 spots were excised to identify by a combination of MALDI-TOF MS after digested with trypsin. The result showed among the 34 proteins identified, plenty of them were involved in metabolism and immunity. Additionally, Heat shock proteins, 30K proteins and Actin were also detected in the fat body of silkworm. The result will provide a useful tool for understanding the role of fat body in silkworm.
The Key Sericultural Laboratory of Agricultural Ministry, Southwest University, Chongqing 400716, China.
Yong
Y
Hou
Ping
P
Zhao
Hong-Li
HL
Liu
Yong
Y
Zou
Jian
J
Guan
Qing-You
QY
Xia
D000273:Adipose Tissue / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D012831:Bombyx / Q000737:chemistry* / Q000235:genetics*; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome