Nucleotide sequence and phylogenetic analysis of a new potexvirus: Malva mosaic virus.
A filamentous virus isolated from Malva neglecta Wallr. (common mallow) and propagated in Chenopodium quinoa was grown, cloned and the complete nucleotide sequence was determined (GenBank accession # DQ660333). The genomic RNA is 6858 nt in length and contains five major open reading frames (ORFs). The genomic organization is similar to members and the viral encoded proteins shared homology with the group of the Potexvirus genus in the Flexiviridae family. Phylogenetic analysis revealed a close relationship with narcissus mosaic virus (NMV), scallion virus X (ScaVX) and, to a lesser extent, to Alstroemeria virus X (AlsVX) and pepino mosaic virus (PepMV). A novel putative pseudoknot structure is predicted in the 3'-UTR of a subgroup of potexviruses, including this newly described virus. The consensus GAAAA sequence is detected at the 5'-end of the genomic RNA and experimental data strongly suggest that this motif could be a distinctive hallmark of this genus. The name Malva mosaic virus is proposed.
Côté
Fabien
F
Centre de recherche en infectiologie, Université Laval (Pavillon CHUL), 2705 boul. Laurier, Québec (QC), Canada, G1V 4G2.
Paré
Christine
C
Majeau
Nathalie
N
Bolduc
Marilène
M
Leblanc
Eric
E
Bergeron
Michel G
MG
Bernardy
Michael G
MG
Leclerc
Denis
D
D001483:Base Sequence; D017124:Conserved Sequence; D028501:Malva / Q000821:virology*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D017863:Potexvirus / Q000145:classification* / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics
D012367:RNA, Viral