Estrogen regulation of the male reproductive tract in the frog, Rana esculenta: a role in Fra-1 activation in peritubular myoid cells and in sperm release.
Endogenous and environmental estrogens have been proved to affect male reproduction in vertebrates. Both positive and negative effects in the regulation of the reproductive tract have been described. Since it is well known that amphibians represent a useful model to study several aspects concerning reproductive activity, we have taken advantage of the frog, Rana esculenta, to study the involvement of estrogens in sperm release. We show here that pituitary hormones increased the number of peritubular myoid cells (PMCs) expressing Fra-1 and induced testicular morphological changes related to sperm release. The estrogen antagonist ICI182-780 counteracted the hypophysis driven effects. In vivo and in vitro experiments demonstrated that 17beta-Estradiol acted directly on the testis to switch-on Fra-1 in PMCs. Furthermore, impairment of estrogen activity significantly reduced sperm release mainly affecting the detachment of spermatozoa from Sertoli cells (spermiation). Therefore, estrogens can be considered a new entry in the list of substances involved in spermiation.
Cobellis
Gilda
G
II Università di Napoli, Dipartimento di Medicina Sperimentale, Via Costrantinopoli 16, 80138 Napoli, Italia.
Cacciola
Giovanna
G
Chioccarelli
Teresa
T
Izzo
Gaia
G
Meccariello
Rosaria
R
Pierantoni
Riccardo
R
Fasano
Silvia
S
D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D005837:Genitalia, Male / Q000187:drug effects* / Q000254:growth & development; D008297:Male; D010903:Pituitary Gland, Anterior / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D010907:Pituitary Hormones / Q000502:physiology; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism / Q000502:physiology*; D011893:Rana esculenta / Q000378:metabolism / Q000502:physiology*; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism*; D014020:Tissue Extracts / Q000494:pharmacology; D000099169:Fos-Related Antigen 1
D004958:Estradiol; D010907:Pituitary Hormones; D016760:Proto-Oncogene Proteins c-fos; D014020:Tissue Extracts; D000099169:Fos-Related Antigen 1