[Hemocompatibility of bovine pericardium with additional sodium bisulfite treatment].
OBJECTIVE
To evaluate the hemocompatibility of glutaraldehyde (GA)-tanned bovine pericardium additionally treated by sodium bisulfite (SOB) solution.


METHODS
The hemocompatibility of GA-tanned bovine pericardium treated by SOB solution is evaluated by using dynamic clotting time test, blood platelet adhension test, D-dimeride determination, and complement activation test. The GA-tanned bovine pericardium was used as control.


RESULTS
The curve of absorbance-clotting time of two kinds of bovine pericardium was similar in dynamic clotting time test. There was no significant difference between SOB-treated and control groups in blood platelet adhension test. The D-dimeride contents of all bioprostheses were at normal level, and the D-dimeride content of GA-tanned bovine pericardium treated by SOB solution was significantly lower than that of control group (P < 0.05). In complement activation test, the level of complement C3a in SOB-treated group was significantly lower than that in control group (P < 0.05).


CONCLUSIONS
GA-tanned bovine pericardium treated by SOB solution meets the demands of cardiac interstitial implanted materials in hemocompatibility.
Han
Mao-sheng
MS
Research Center for Cardiovascular Regeneration Medicine of the Ministry of Health, Cardiovascular Institute and Fuwai Hospital, CAMS and PUMC, Beijing 100037, China.
Zhou
Jian-ye
JY
Hu
Sheng-shou
SS
Wang
Sheng
S
Jiang
Hong
H
D000818:Animals; D001672:Biocompatible Materials; D001705:Bioprosthesis; D001777:Blood Coagulation; D002417:Cattle; D015926:Complement C3a / Q000032:analysis; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism; D005976:Glutaral / Q000494:pharmacology; D008422:Materials Testing; D010496:Pericardium / Q000187:drug effects* / Q000378:metabolism; D010973:Platelet Adhesiveness; D013447:Sulfites / Q000494:pharmacology*
D001672:Biocompatible Materials; D005338:Fibrin Fibrinogen Degradation Products; D013447:Sulfites; C036309:fibrin fragment D; D015926:Complement C3a; D005976:Glutaral; C009279:sodium bisulfite