A simple and sensitive liquid chromatography-tandem mass spectrometry assay for the quantification of ertapenem in microdialysate.
A new liquid chromatography assay with isocratic elution and tandem mass spectrometry detection (LC-MS/MS) using an electrospray ionization interface in the multiple reaction monitoring mode was developed and validated for ertapenem determination in microdialysate samples. Linearity was demonstrated between 10ngmL(-1) (lower limit of quantification, LLoQ) and 160ngmL(-1). The precision (CV%) and accuracy (bias%) in microdialysates at the LLoQ were respectively 2.2% and 17.3% within-day and 10.6% and 2.7% between-days. Ertapenem was stable for 1 month at -20 degrees C and -80 degrees C but unstable at +4 degrees C. This new LC-MS/MS assay is simple, rapid and more sensitive than previously described assays.
INSERM, ERI 23, 40 Avenue du Recteur Pineau, Poitiers, France.
Sandrine
S
Lefeuvre
Nicolas
N
Venisse
Sandrine
S
Marchand
Mickaël
M
Bachelet
William
W
Couet
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D000077727:Ertapenem; D017551:Microdialysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D047090:beta-Lactams / Q000032:analysis*
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D000077727:Ertapenem