Molecular detection of Ureaplasma urealyticum infection from clinical urogenital swabs.
A simple nucleic acid amplification test (NAAT) was developed for detection of Ureaplasma urealyticum infection based on the PCR amplification of the urease gene (UU1/UU2 Test). DNA was extracted from urogenital swabs and a 225-bp long DNA fragment was amplified by PCR. NAAT was compared to the commercial amplification kit for sexually transmitted disease reference assay. The sensitivity and specificity of the UU1/UU2 Test were determined to be 100 and 98.9%, respectively. The overall prevalence rate in this group of patients was found to be about 236 per 1000 (283 and 166 per 1000 in females and males, respectively). These data demonstrate that UU1/UU2 Test is suitable for effective epidemiological screening and/or diagnostic practice.
Department of Pharmacology, Faculty of Medicine, P.J. Safárik University, 040 66 Kosice, Slovakia.
E
E
Biros
J
J
Bodnár
I
I
Biros
E
E
Birosová
J
J
Mojzis
M
M
Hrivnák
L
L
Klimcáková
I
I
Findlay
A
A
Mirossay
L
L
Mirossay
D000293:Adolescent; D000328:Adult; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D052776:Female Urogenital Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D008297:Male; D052801:Male Urogenital Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D020539:Sequence Analysis, Protein; D018154:Slovakia / Q000453:epidemiology; D016869:Ureaplasma Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D016990:Ureaplasma urealyticum / Q000235:genetics / Q000302:isolation & purification*; D014510:Urease / Q000737:chemistry / Q000235:genetics
D004269:DNA, Bacterial; D014510:Urease