Antioxidant effect of 5-amino salicylic acid on copper-mediated LDL oxidation.
The antioxidant effect of 5-Aminosalicylic acid (5-ASA) on copper-mediated LDL oxidation was followed either by the emitted chemiluminiscence (CL) or by UV-vis spectroscopy. 5-ASA addition extends the lag phase in a concentration-dependent manner without changes in the rate of the process in the autoaccelerated phase. The antioxidant behavior of 5-ASA was very similar to that of Trolox, a very efficient water soluble antioxidant. The copper-binding capacity of 5-ASA was evaluated by UV-visible spectroscopy. The addition of copper to a 5-ASA solution increases the absorbance at 332 nm and generates a new band at 298 nm. These changes in the UV-vis spectra indicate formation of a complex between 5-ASA and copper. However, LDL protection by 5-ASA is unrelated to its copper chelating capacity.
Departamento de Farmacia, Facultad de Química, Pontificia Universidad Católica de Chile, Santiago, Chile.
Camilo
C
López-Alarcón
Hernán
H
Speisky
Eduardo
E
Lissi
D000636:Aminosalicylic Acids / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D003300:Copper / Q000737:chemistry* / Q000633:toxicity; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008163:Luminescent Measurements; D010084:Oxidation-Reduction / Q000187:drug effects; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D000636:Aminosalicylic Acids; D000975:Antioxidants; D008077:Lipoproteins, LDL; D003300:Copper