Immunohistochemical detection of porcine teschovirus antigen in the formalin-fixed paraffin-embedded specimens from pigs experimentally infected with porcine teschovirus.
Porcine teschovirus (PTV) antigens were detected by a streptavidin-biotin complex method in formalin-fixed paraffin-embedded tissues of 3-week-old pigs that had been inoculated intravenously with PTV Talfan strain. PTV antigens were detected in cytoplasm of nerve cells, glial cells and endothelial cells in the cerebellar nuclei, the grey matter of the midbrain, pons and medulla oblongata and the ventral horn of the spinal cord and of ganglion cells in the spinal ganglion corresponding to those lesions characterized as non-suppurative encephalomyelitis and ganglionitis. The results of this study suggest that nerve cells of the brain stem and spinal cord and ganglion cells of the spinal ganglion permit PTV replication and represent the main target cell population of PTV. This is the first study to demonstrate PTV antigen by immunohistochemistry in formalin-fixed paraffin-embedded tissue specimens from pigs infected with PTV.
Yamada
M
M
National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan. oomae@affrc.go.jp
Kaku
Y
Y
Nakamura
K
K
Yoshii
M
M
Yamamoto
Y
Y
Miyazaki
A
A
Tsunemitsu
H
H
Narita
M
M
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D005557:Formaldehyde; D007150:Immunohistochemistry / Q000662:veterinary; D010232:Paraffin; D010850:Picornaviridae Infections / Q000473:pathology / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000473:pathology*; D052700:Teschovirus / Q000276:immunology*
D000956:Antigens, Viral; D005557:Formaldehyde; D010232:Paraffin