Fluorescent serum and urinary advanced glycoxidation end-products in non- diabetic subjects.
BACKGROUND
Advanced glycoxidation end-products (AGEs) are involved in age-related conditions and diabetic complications. Diet intake contributes to their circulating concentrations.


OBJECTIVE
To measure serum and urinary AGEs in non-diabetic volunteers and relate their concentration to body composition, blood chemistry and dietary ingestion.


METHODS
We studied 41 adult men (31 middle-aged adults and 10 elderly). A nutritional assessment including a dietary recall designed for detection of AGE ingestion (specifically carboxymethyl-lysine(CML)), and anthropometric measurements were performed. Also serum lipoproteins, insulin, glucose, leptin and C reactive protein (CRP). AGEs were measured in serum and urine samples using size exclusion chromatography and flow injection assay (FIA); the technical procedures were first employed in 11 heterogeneous diabetics, as positive controls for this methodology.


RESULTS
Serum and urinary chromatograms indicated that areas under the curve were not different in younger compared with elderly adults. AGEs did not correlate with dietary intake, body composition, nor metabolic parameters, however they correlated significantly with renal function and CRP concentration.


CONCLUSIONS
In these non-diabetic volunteers, with low CML intake, serum and urinary concentration of AGEs were not related to dietary intake. AGEs were related to renal function and CRP, but not to body composition, lipoproteins, insulin and glucose.
Maza
M P de la
MP
Institute of Nutrition and Food Technology (INTA), University of Chile, Chile. mpmaza@inta.cl
Bravo
A
A
Leiva
L
L
Gattás
V
V
Petermann
M
M
Garrido
F
F
Bunout
D
D
Hirsch
S
S
Barrera
G
G
Fernández
M
M
D000328:Adult; D000368:Aged; D001823:Body Composition; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D005453:Fluorescence; D005947:Glucose / Q000032:analysis; D017127:Glycation End Products, Advanced / Q000008:administration & dosage / Q000097:blood* / Q000652:urine*; D006801:Humans; D008074:Lipoproteins / Q000097:blood; D008239:Lysine / Q000008:administration & dosage / Q000031:analogs & derivatives; D008297:Male; D008875:Middle Aged; D013050:Spectrometry, Fluorescence
D017127:Glycation End Products, Advanced; D008074:Lipoproteins; C048496:N(6)-carboxymethyllysine; D005947:Glucose; D008239:Lysine