[Association between single nucleotide polymorphism of ADFP gene and carcass traits and intramuscular fat content in chicken].
In order to investigate the effect of ADFP gene on the carcass traits and intramuscular fat content of Da-heng high-quality chicken, we analyzed the coding sequence of the ADFP gene by single-strand conformation polymorphism (SSCP) and DNA sequencing in five pure lines and three crossbreds. We found three single nucleotide polymorphism sites, including A/T at base position 4,079 (locus A), C/T at base position 4,843 (locus B) and A/G at base position 7,070 (locus C). GLM analysis for the effect of genotypes on carcass traits and intramuscular fat content demonstrated a significant effect on leg muscle percentage, abdomen fat weight, abdominal fat percentage and intra-muscular fat content in locus A (P < 0.05), a significant association with living body weight and carcass weight for locus B (P < 0.05) and a significant association with breast muscle weight and intramuscular fat content (P < 0.05), and a very significant association with breast muscle percentage for locus C (P < 0.01). It was concluded that ADFP gene may be a major gene or linked to such a gene that affects the fat traits of birds. Polymorphic loci A and C could be used as molecular markers for abdominal fat weight, abdominal fat percentage and intramuscular fat content in birds in future studies.
College of Animal Science, Sichuan Agricultural University, Ya'an 625014, China. zhaoxiaoling04@163.com
Xiao-Ling
XL
Zhao
Yi-Ping
YP
Liu
Liang
L
Li
Xiao-Song
XS
Jiang
Hua-Rui
HR
DU
Qing
Q
Zhu
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism; D002645:Chickens / Q000033:anatomy & histology* / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006720:Homozygote; D008297:Male; D008565:Membrane Proteins; D009132:Muscles; D010455:Peptides / Q000235:genetics*; D000070780:Perilipin-2; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D012984:Software
D015415:Biomarkers; D008565:Membrane Proteins; D010455:Peptides; D000070780:Perilipin-2