Fast and direct quantification of adrenal steroids by tandem mass spectrometry in serum and dried blood spots.
We present a fast and reproducible method for steroid analysis (corticosterone, deoxycorticosterone, progesterone, 17alpha-hydroxyprogesterone, 11-deoxycortisol, 21-deoxycortisol, androstenedione, testosterone, dihydrotestosterone and cortisol) in small volumes of serum and in dried blood spot samples by LC-MS/MS. No derivatisation was needed. LC separation was achieved by using an Atlantis C18 column and water-methanol-formic acid gradient as a mobile phase and a flow rate of 250 microL/min over a run time of 6 min. Steroids were measured in MRM mode with electrospray interface (positive ion mode). Validation showed excellent precision, sensitivity, recovery and linearity with coefficients of determination r2>0.992.
Janzen
Nils
N
Screening-Laboratory Hannover, c/o Box 91 10 09, Hannover D-30430, Germany. n.janzen@metabscreen.de
Sander
Stefanie
S
Terhardt
Michael
M
Peter
Michael
M
Sander
Johannes
J
D000311:Adrenal Glands / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D013256:Steroids / Q000032:analysis / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D013256:Steroids