Putative palytoxin and its new analogue, ovatoxin-a, in Ostreopsis ovata collected along the Ligurian coasts during the 2006 toxic outbreak.
In this article we report on the liquid chromatography tandem mass spectrometry (LC-MS) investigation of plankton samples collected in the summer of 2006 along the Ligurian coasts, coinciding with a massive bloom of the tropical microalga Ostreopsis ovata. LC-MS analyses indicated the occurrence of putative palytoxin along with a much more abundant palytoxin-like compound never reported so far, which we named ovatoxin-a. On the basis of molecular formula, fragmentation pattern, and chromatographic behavior, the structure of ovatoxin-a appeared to be strictly related to that of palytoxin. We report also on the analysis of cultured O. ovata, which was necessary to unequivocally demonstrate that putative palytoxin and ovatoxin-a contained in field samples were actually produced by O. ovata itself.
Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli Federico II, Napoli, Italy.
Patrizia
P
Ciminiello
Carmela
C
Dell'Aversano
Ernesto
E
Fattorusso
Martino
M
Forino
Luciana
L
Tartaglione
Claudio
C
Grillo
Nunzia
N
Melchiorre
D000178:Acrylamides / Q000032:analysis / Q000737:chemistry*; D000818:Animals; D002853:Chromatography, Liquid; D003064:Cnidarian Venoms / Q000032:analysis / Q000737:chemistry*; D004141:Dinoflagellida / Q000737:chemistry*; D056890:Eukaryota / Q000737:chemistry* / Q000378:metabolism; D007558:Italy; D008522:Mediterranean Sea; D010839:Phytoplankton / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D017550:Spectroscopy, Fourier Transform Infrared; D053719:Tandem Mass Spectrometry; D014873:Water Pollutants / Q000032:analysis / Q000737:chemistry*; D000095702:Polyether Toxins
D000178:Acrylamides; D003064:Cnidarian Venoms; D014873:Water Pollutants; C010272:palytoxin; D000095702:Polyether Toxins