Photochemically induced RNA and DNA abasic sites.
Two phosphoramidite building blocks were synthesized that can easily be deprotected by UV light to reveal natural abasic sites in oligoribonucleotides as well as in oligodeoxyribonucleotides. Another building block which releases a 2'-O-methylated abasic site upon UV radiation is also described.
Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland.
Pascal A
PA
Küpfer
Christian J
CJ
Leumann
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D007700:Kinetics; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000528:radiation effects; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000528:radiation effects; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000528:radiation effects; D010777:Photochemistry; D012313:RNA / Q000737:chemistry* / Q000528:radiation effects*; D014466:Ultraviolet Rays
D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D012313:RNA; D004247:DNA