Amentoflavone, a biflavonoid from Biophytum sensitivum augments lymphocyte proliferation, natural killer cell and antibody dependent cellular cytotoxicity through enhanced production of IL-2 and IFN-gamma and restrains serum sialic acid and gamma glutamyl transpeptidase production in tumor - bearing animals.
Modulation of immune response is highly relevant in tumor cell destruction. The present study is focused on the effect of amentoflavone, a biflavonoid from Biophytum sensitivum on cell-mediated immune responses in normal and tumor-bearing control animals. Tumor was induced in BALB/c mice by intraperitoneal injection of Ehrlich ascites carcinoma cells. Treatment of amentoflavone significantly enhanced natural killer cell activity in normal (42.8% cell lysis) and tumor bearing animals (48.2% cell lysis) on the fifth day, which was much earlier compared to tumor-bearing control animals (20.2% cell lysis on day 9). Antibody-dependent cellular cytotoxicity was also increased in amentoflavone -treated normal (41% cell lysis on day 9) and tumor bearing animals (43.8% cell lysis on day 9) compared to untreated tumor bearing control animals (maximum of 15.2% cell lysis on day 13). Amentoflavone administration could significantly enhance the mitogen-induced splenocyte, thymocyte, and bone marrow cell proliferation. Treatment of amentoflavone significantly elevated the production of interleukin-2 and interferon-gamma in normal and Ehrlich ascites carcinoma-bearing animals. Moreover amentoflavone treatment significantly reduced the elevated levels of serum sialic acid and serum gamma glutamyl transpeptidase activity in tumor bearing animals.
Guruvayoorappan
Chandrasekharan
C
Department of Immunology, Amala Cancer Research Center, Amala Nagar, Thrissur 680 555, Kerala State, India.
Kuttan
Girija
G
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects*; D044946:Biflavonoids / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019158:N-Acetylneuraminic Acid / Q000097:blood*; D005723:gamma-Glutamyltransferase / Q000096:biosynthesis / Q000097:blood*
D044946:Biflavonoids; D007376:Interleukin-2; D007371:Interferon-gamma; C011164:amentoflavone; D005723:gamma-Glutamyltransferase; D019158:N-Acetylneuraminic Acid