Effectiveness of various Pseudomonas spp. and Burkholderia caryophylli containing ACC-deaminase for improving growth and yield of wheat (Triticum aestivum L.).
This study assessed the possible role of different traits in selected plant growth-promoting rhizobacteria (PGPR) for improving wheat growth and yield under natural conditions. Rhizobacteria exhibiting 1-aminocyclopropane-1-carboxylate (ACC)-deaminase activity were isolated and screened for their growth-promoting activity in wheat under axenic conditions. Five isolates belonging to Pseudomonas and one Burkholderia caryophylli isolate that showed promising performances under axenic conditions were selected and characterized for in vitro ACC-deaminase activity, chitinase activity, auxin production, P solubilization, and root colonization. These isolates were then used as inocula for wheat cultivated under natural conditions in pot and/or field trials. Significant increases in root elongation, root weight, tillers per pot, 1,000-grain weight, and grain and straw yields were observed in response to inoculation with PGPR in the pot trials. Inoculation with these PGPR was also effective under field conditions and increased the wheat growth and yield significantly. However, the efficacy of the strains was inconsistent under the axenic, pot, and field conditions. Pseudomonasfluorescens (ACC50), which exhibited a relatively high in vitro ACC-deaminase activity, chitinase activity, auxin production, and P solubilization and more intensive root colonization, was the most efficient isolate under the field conditions. Therefore, these results demonstrated that ACC-deaminase activity is an efficient parameter for the selection of promising PGPR under axenic conditions. However, additional traits of PGPR, including auxin production, chitinase activity, P solubilization, and root colonization, are also important for selecting PGPR as biofertilizers.
Institute of Soil & Environmental Sciences, University ofAgriculture, Faisalabad-38040, Pakistan.
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B
Shaharoona
G M
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Jamro
Z A
ZA
Zahir
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M
Arshad
K S
KS
Memon
D018533:Biomass; D019117:Burkholderia / Q000201:enzymology* / Q000302:isolation & purification / Q000502:physiology; D019755:Carbon-Carbon Lyases / Q000096:biosynthesis*; D002688:Chitinases / Q000096:biosynthesis; D002523:Edible Grain / Q000254:growth & development; D007210:Indoleacetic Acids / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D011549:Pseudomonas / Q000201:enzymology* / Q000302:isolation & purification / Q000502:physiology; D014908:Triticum / Q000254:growth & development* / Q000382:microbiology*
D007210:Indoleacetic Acids; D010710:Phosphates; D002688:Chitinases; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases