Improvement in rabbit corneal cell suspension viability after freezing with Gingko Biloba extract.
We investigated whether the addition of Gingko Biloba extract (EGb 761) to rabbit corneal epithelial medium before cell freezing improved cell viability after freezing then thawing. After removal of corneas, they were treated with enzymes and the corneal epithelium was prepared as a single cell suspension in freezing media with or without EGb 761. After freezing for two weeks then thawing, a higher cell viability was found in the cornea cell suspensions which had been frozen pretreated with EGb 761 in the media. The improvement with corneal cell viability with EGb 761 pretreatment is postulated to be based on the antioxidant capacity of the plant extract.
Aktan
Murad
M
Selcuk University, Meram Medical Faculty, Histology-Embryology Department, Konya, Turkey. muradaktan@gmail.com
Okka
Berrin
B
Okka
Mehmet
M
Duman
Selcuk
S
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology*; D019573:Epithelium, Corneal / Q000166:cytology* / Q000187:drug effects; D020441:Ginkgo biloba; D066298:In Vitro Techniques; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D011817:Rabbits; D000096983:Ginkgo Extract
D003451:Cryoprotective Agents; D010936:Plant Extracts; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract