Production of cross-reactive peptide antibodies against viral capsid proteins of human enterovirus B to apply diagnostic reagent.
The coxsackievirus group B (CVB) of the genus Enterovirus and the species human enterovirus B is a nonenveloped virus containing a single-stranded positive-sense RNA genome. Coxsackievirus has icosahedral symmetry and four capsid proteins, VP1, VP2, VP3, and VP4. Specific antibodies against each viral protein are prerequisites for various studies. In this study, we developed seven peptide-derived antibodies directed against coxsackievirus VP1 (NO1-NO5), VP2 (B3), and VP3 (GL3). We developed a type-specific antibody (NO1) and broadly cross-reactive antibodies (NO3 and NO5) to VP1. Anti-VP2 and anti-VP3 antibodies (B3 and GL3, respectively) are also cross-reactive to human enterovirus B such as CVB and echoviruses. Their sensitivities and reactivities are likely to be better than those of the commercial VP1 monoclonal antibody (MAb). The dot-blot analysis also showed that NO5 against VP1 is able to detect less than 1 microg [2x10(6) plaque-forming unit (pfu) of CVB3] of viruses, suggesting that it could be used to develop a diagnostic kit that can directly detect human enterovirus B. The antibodies produced here may allow us to undertake several studies, such as those involving viral trafficking, expression kinetics, and the roles of viral proteins in infection, and the development of diagnostic kits.
Jeong
Soo-Young
SY
Department of Biotechnology, The Catholic University of Korea, Bucheon, Korea.
Ahn
Jeonghyun
J
Cho
Young-Joo
YJ
Kim
Yeun-Jung
YJ
Kim
Dae-Sun
DS
Jee
Youngmee
Y
Lee
Heuiran
H
Nam
Jae-Hwan
JH
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000235:genetics; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000737:chemistry* / Q000276:immunology*; D003384:Coxsackievirus Infections / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D003429:Cross Reactions; D029822:Enterovirus B, Human / Q000276:immunology / Q000302:isolation & purification; D006367:HeLa Cells; D006801:Humans; D010446:Peptide Fragments / Q000276:immunology*; D010455:Peptides / Q000276:immunology; D012367:RNA, Viral / Q000032:analysis
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D010446:Peptide Fragments; D010455:Peptides; D012367:RNA, Viral