Partial sequencing and expression of genes involved in glucose metabolism in adipose tissues and skeletal muscle of healthy cats.
Impaired insulin sensitivity is increasingly recognised in cats, but sequences of genes involved in insulin-signalling are largely undetermined in this species. In this study, extended feline mRNA sequences were determined for the adiponectin, glucose transporter-1 (GLUT1), GLUT4, peroxisome proliferative activated receptor-gamma1 (PPARgamma1), PPARgamma2, plasminogen activator inhibitor-1 (PAI-1), monocyte chemoattractant protein-1 (MCP-1) and insulin receptor genes. Conserved dog-specific primers identified from human-dog mRNA alignments were used to amplify feline cDNA in the polymerase chain reaction (PCR). The feline sequences determined by this method were used to design feline-specific primers suitable for real-time PCR for quantification of gene expression in insulin sensitive tissues of healthy cats. Partial sequences of feline mRNAs had 86-95% identity with dog and human genes. Expression of adiponectin, GLUT1, GLUT4, PPARgamma1, PPARgamma2, PAI-1 and insulin receptor mRNA was detected and quantified in subcutaneous and visceral fat and skeletal muscle, whereas MCP-1 mRNA was detected in adipose tissue but not in skeletal muscle. Further characterisation of genes related to glucose metabolism in cats will provide additional insights into insulin-signalling mechanisms in this species.
Zini
Eric
E
Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zürich, Winterthurerstr. 260, 8057 Zürich, Switzerland. ezini@vetclinics.uzh.ch
Linscheid
Philippe
P
Franchini
Marco
M
Kaufmann
Karin
K
Monnais
Edouard
E
Kutter
Annette P
AP
Ackermann
Mathias
M
Lutz
Thomas A
TA
Reusch
Claudia E
CE
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D002415:Cats / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism / Q000235:genetics*; D005260:Female; D015870:Gene Expression / Q000235:genetics; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000235:genetics*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D047495:PPAR gamma / Q000235:genetics; D011972:Receptor, Insulin / Q000235:genetics; D013045:Species Specificity
D001786:Blood Glucose; D051246:Glucose Transport Proteins, Facilitative; D007328:Insulin; D047495:PPAR gamma; D011972:Receptor, Insulin