Channel wall coating on a poly-(methyl methacrylate) CE microchip by thermal immobilization of a cellulose derivative for size-based protein separation.
We demonstrate channel wall coating using a cellulose derivative on a poly-(methyl methacrylate) (PMMA) CE microchip to eliminate EOF disturbing protein separation. The channel walls were modified by preconditioning with a solution containing the cellulose derivative and then thermally evaporating the solution to produce hydrophilic channel walls which prevent adsorption of analytes via a hydrophobic interaction. When the PMMA substrate was coated with the cellulose derivative hydroxypropylmethylcellulose (HPMC) 90SH, the water contact angle on the coated substrate was decreased (up to 15 degrees ) and EOF was significantly suppressed (up to 4.0 x 10(-6) cm2.V(-1)s(-1)). Three proteins (20.5, 68.0, and 114.6 kDa) were successfully separated on the 0.15% HPMC 90SH-coated channel walls with good reproducibility of migration time (RSD <1.75%) and high efficiency (theoretical plate number per meter: 2.62 x 10(5)).
Okada
Hiroki
H
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya, Japan. h051102d@mbox.nagoya-u.ac.jp
Kaji
Noritada
N
Tokeshi
Manabu
M
Baba
Yoshinobu
Y
D002482:Cellulose / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011506:Proteins / Q000302:isolation & purification*; D012996:Solutions; D013237:Stereoisomerism
D011506:Proteins; D012996:Solutions; D002482:Cellulose; D019904:Polymethyl Methacrylate