Enzyme-catalyzed signal amplification for electrochemical DNA detection with a PNA-modified electrode.
The signal amplification technique of peptide nucleic acid (PNA)-based electrochemical DNA sensor was developed in a label-free and one-step method utilizing enzymatic catalysis. Electrochemical detection of DNA hybridization on a PNA-modified electrode is based on the change of surface charge caused by the hybridization of negatively charged DNA molecules. The negatively charged mediator, ferrocenedicarboxylic acid, cannot diffuse to the DNA hybridized electrode surface due to the charge repulsion with the hybridized DNA molecule while it can easily approach the neutral PNA-modified electrode surface without the hybridization. By employing glucose oxidase catalysis on this PNA-based electrochemical system, the oxidized mediator could be immediately reduced leading to greatly increased electrochemical signals. Using the enzymatic strategy, we successfully demonstrated its clinical utility by detecting one of the mutation sequences of the breast cancer susceptibility gene BRCA1 at a sample concentration lower than 10(-9) M. Furthermore, a single base-mismatched sample could be also discriminated from a perfectly matched sample.
Won
Byoung Yeon
BY
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Yoon
Hyun C
HC
Park
Hyun Gyu
HG
D000818:Animals; D015374:Biosensing Techniques; D002384:Catalysis; D004247:DNA / Q000032:analysis*; D004563:Electrochemistry; D004566:Electrodes; D005949:Glucose Oxidase / Q000378:metabolism; D020135:Peptide Nucleic Acids
D020135:Peptide Nucleic Acids; D004247:DNA; D005949:Glucose Oxidase