Mapping cynomolgus monkey MHC class I district on chromosome 6p13 using pooled cDNAs.
The cynomolgus monkey (Macaca fascicularis) is a frequently used animal model for studying human diseases, especially immune related ones. For a better understanding of its major histocompatibility complex (MHC) class I district chromosome location, we selected seven cDNA clones as probes for fluorescence in situ hybridization (FISH) from a lymphocyte cell line cDNA library. Expressed sequence tags (ESTs) from these clones were assembled into three clusters and annotated Mafa-A and Mafa-B genes. Further bioinformatics analysis shows that they had multiple duplications spanning approximately 2.8 Mb on the rhesus macaque MHC class I district. Using the FISH technique, we mapped the seven pooled cDNA clones to the short arm of the cynomolgus monkey chromosome 6 on 6p13. To our knowledge, this is the first report of the location of cynomolgus monkey MHC class I district. Using pooled adjacent cDNAs as probes also allows affordable, specific genome region mapping research.
Liu
Q Y
QY
Beijing Institute of Genomics, Beijing, China.
Wang
X X
XX
Zhang
J Z
JZ
Chen
W H
WH
He
X W
XW
Lin
Y
Y
Wang
J F
JF
Zhu
Y
Y
Hu
S N
SN
Wang
X N
XN
D000818:Animals; D002871:Chromosome Banding / Q000379:methods*; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics* / Q000648:ultrastructure; D020451:Contig Mapping / Q000379:methods*; D015723:Gene Library; D005805:Genes, MHC Class I / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008252:Macaca fascicularis / Q000235:genetics*