Annexin A2 phosphorylation mediates cell scattering and branching morphogenesis via cofilin Activation.
Dynamic remodeling of the actin cytoskeleton is required for cell spreading, motility, and migration and can be regulated by tyrosine kinase activity. Phosphotyrosine proteomic screening revealed phosphorylation of the lipid-, calcium-, and actin-binding protein annexin A2 (AnxA2) at Tyr23 as a major event preceding ts-v-Src kinase-induced cell scattering. Expression of the phospho-mimicking mutant Y23E-AnxA2 itself was sufficient to induce actin reorganization and cell scattering in MDCK cells. While Y23E-AnxA2, but not Y23A-AnxA2, enhanced Src- or hepatocyte growth factor (HGF)-induced cell scattering, short hairpin RNA-mediated knockdown of AnxA2 inhibited both v-Src- and HGF-induced cell scattering. Three-dimensional branching morphogenesis was induced in wild-type-AnxA2-expressing cells only in the presence of HGF, while Y23E-AnxA2 induced HGF-independent branching morphogenesis. Knockdown of AnxA2 prevented lumen formation during cystogenesis. The Y23E-AnxA2-induced scattering was associated with dephosphorylation/activation of the actin-severing protein cofilin. Likewise, inactive S3E-cofilin and constitutively active LIM kinase, a direct upstream kinase of cofilin, inhibited Y23E-AnxA2-induced scattering. Together, our studies indicate an essential role for AnxA2 phosphorylation in regulating cofilin-dependent actin cytoskeletal dynamics in the context of cell scattering and branching morphogenesis.
de Graauw
Marjo
M
Division of Toxicology, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratoria, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
Tijdens
Ine
I
Smeets
Mirjam B
MB
Hensbergen
Paul J
PJ
Deelder
André M
AM
van de Water
Bob
B
D000818:Animals; D017306:Annexin A2 / Q000378:metabolism*; D002460:Cell Line; D048430:Cell Shape; D051338:Cofilin 1 / Q000378:metabolism*; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology*; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D054461:Lim Kinases / Q000502:physiology; D010766:Phosphorylation
C472822:ANXA2 protein, human; D017306:Annexin A2; D051338:Cofilin 1; D017228:Hepatocyte Growth Factor; D054461:Lim Kinases