Homogeneous fluorescence assays for RNA diagnosis by pyrene-conjugated 2'-O-methyloligoribonucleotides.
We developed a bispyrene-conjugated 2'-O-methyloligoribonucleotide as an RNA-specific RNA-probe. The probe hybridized with the complementary RNA, greatly enhancing fluorescence and discriminating RNA from DNA. The assay was carried out in homogeneous aqueous media without removing the unbound probe from the detection solution. This homogeneous fluorescence assay also discriminated mismatch sequences in the target RNA. These pyrene probes could possess high potential to detect RNA in biological specimens simply.
Sakamoto
Takashi
T
Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsungsaki, Kyoto 606-8585, Japan.
Kobori
Akio
A
Shigezawa
Masako
M
Amitani
Yasutaka
Y
Higuchi
Maiko
M
Murakami
Akira
A
D003562:Cytidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D008745:Methylation; D009693:Nucleic Acid Hybridization; D009843:Oligoribonucleotides / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry; D015347:RNA Probes / Q000737:chemistry*; D018075:RNA, Complementary / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014529:Uridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
C527210:2'-O-pyrenylmethylcytidine; C527209:2'-O-pyrenylmethyluridine; D009843:Oligoribonucleotides; D011721:Pyrenes; D015347:RNA Probes; D018075:RNA, Complementary; D003562:Cytidine; C030984:pyrene; D014529:Uridine