title: HPV status and regional metastasis in the prognosis of oral and oropharyngeal cancer.
abstract: Prognostic factors are important for treatment decisions as they help adapt the therapy on a case-to-case basis. Nodal status, number of positive nodes, and presence of extracapsular spread are considered to be the important prognostic factors in head and neck cancer. Some studies suggest that human papillomavirus (HPV) status also influences the outcome of the treatment. This influence can be explained by the variation in tendency to develop regional metastases and by variation in the type of neck node involvement. The study objectives were to compare patients with HPV positive and HPV-negative tumors for survival and prevalence and type of regional metastasis, to identify prognostic factors and to test whether HPV presence is an independent factor of survival. The study included 81 patients treated by surgery including neck dissection for oral or oropharyngeal squamous cell cancer. A computerized medical report was completed for each patient. Analysis of the tumor specimen for the HPV DNA presence was done on paraffin-fixed tissue. HPV DNA detection and typing were performed by PCR with GP5+/GP6+BIO primers and reverse line blot hybridization. Overall, 64% (52/81) of tumors were HPV positive with 80% in the tonsillar site. HPV-positive patients had significantly better both overall (73 vs. 35%) (P=0.0112) and disease-specific (79 vs. 45%) (P=0.0015) survival rates than HPV-negative patients. No significant differences were found in the pN classification, in the number of positive nodes and the presence of extracapsular spread in the involved nodes between HPV positive and HPV-negative tumors. Multivariate analysis showed that significant prognostic factors of survival were the presence of HPV in the tumor, extracapsular spread and tumor size. HPV was the most significant prognostic factor in the studied group of patients with oropharyngeal tumors (HR=0.27, 95%CI 0.12-0.61) and possibly should be considered in treatment decisions.
authors: Department of Otolaryngology Head and Neck Surgery, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
authors: Jan
authors: J
authors: Klozar
authors: Vit
authors: V
authors: Kratochvil
authors: Martina
authors: M
authors: Salakova
authors: Jana
authors: J
authors: Smahelova
authors: Eva
authors: E
authors: Vesela
authors: Eva
authors: E
authors: Hamsikova
authors: Jan
authors: J
authors: Betka
authors: Ruth
authors: R
authors: Tachezy
mesh_terms: D052685:Alphapapillomavirus / Q000235:genetics / Q000302:isolation & purification*; D018153:Czech Republic / Q000453:epidemiology; D004279:DNA, Viral / Q000737:chemistry; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000401:mortality* / Q000473:pathology / Q000821:virology*; D009959:Oropharyngeal Neoplasms / Q000401:mortality* / Q000473:pathology / Q000821:virology*; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012307:Risk Factors; D016019:Survival Analysis
chemical_list: D004279:DNA, Viral