Isolation and characterization of nonrhizobial plant growth promoting bacteria from nodules of Kudzu (Pueraria thunbergiana) and their effect on wheat seedling growth.
The leguminous vine Kudzu (Pueraria thunbergiana) is an introduction into the N. W. Himalayan region of India. Despite its value as a fodder and cover crop, little is known about the nature of the nodule microflora. In an attempt to study the nodule bacteria, we isolated and characterized three nonrhizobial plant growth promoting bacteria from surface sterilized nodules of Kudzu. Based on the sequencing of the 16 S r RNA gene, the isolates were designated as Bacillus thuringiensis KR-1, Enterobacter asburiae KR-3, and Serratia marcescens KR-4. Crystalline bodies were detected in the isolate KR-1, confirming its identity as B. thuringiensis. Under in vitro conditions, all three isolates were found to produce indole acetic acid. Other plant growth promotion attributes such as P solubilization, hydrogen cyanide production, and ammonia production varied among the isolates. All of the three isolates promoted growth and positively influenced nutrient uptake parameters of wheat seedlings.
Selvakumar
G
G
Vivekananda Institute of Hill Agriculture, Indian Council of Agricultural Research, Almora 263601, Uttarakhand, India. gselva74@rediffmail.com
Kundu
S
S
Gupta
Anand D
AD
Shouche
Yogesh S
YS
Gupta
Hari S
HS
D001413:Bacillus thuringiensis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004754:Enterobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D029908:Pueraria / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology*; D036226:Seedlings / Q000254:growth & development* / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012706:Serratia marcescens / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012988:Soil Microbiology; D014908:Triticum / Q000254:growth & development* / Q000382:microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S