title: Alternatively spliced forms of the P180 ribosome receptor differ in their ability to induce the proliferation of rough endoplasmic reticulum.
abstract: Expression of the canine 180-kDa ribosome receptor p180 in yeast induces the synthesis of RER, and increases the mRNAs of secretory pathway proteins, and protein secretion. To assess whether p180 is a master regulator of cell secretion in mammalian cells, we stably expressed red fluorescent forms of the human p180 variants p180DeltaR (no tandem repeats), p180R (26 repeats), and full-length p180FR (54 repeats) containing different lengths of the tandem repeat ribosome-binding domain in rat pancreatic RINm5F islet beta-cells. All three fluorescent p180 variants localized exclusively to the RER. Cells transfected with p180R were filled with ribosome-studded karmellae, whereas p180DeltaR and p180FR transfectants contained only increased amounts of mostly smooth ER. Unlike in yeast, over-expression of p180R failed to increase the secretory pathway proteins calnexin, SEC61beta, and calreticulin, or ribosome biogenesis. The data suggest that alternative splicing of the p180 tandem repeat domain is a means of regulating the ribosome-binding activity of p180, and potentially the secretory activity of the cell. However, p180 is not a master regulator of mammalian cell secretion as it does not concomitantly trigger the synthesis of protein machinery required to enhance protein synthesis and cell secretion.
authors: Department of Molecular Medicine & Pathology, School of Medical Sciences, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand.
authors: Ji-Zhong
authors: JZ
authors: Bai
authors: Euphemia
authors: E
authors: Leung
authors: Hilary
authors: H
authors: Holloway
authors: Geoffrey W
authors: GW
authors: Krissansen
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002460:Cell Line; D018870:Endoplasmic Reticulum, Rough / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D006801:Humans; D050417:Insulin-Secreting Cells / Q000378:metabolism; D021381:Protein Transport / Q000235:genetics; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D022142:Secretory Vesicles / Q000378:metabolism; D020080:Tandem Repeat Sequences; D002352:Carrier Proteins
chemical_list: D049452:Green Fluorescent Proteins; D018160:Receptors, Cytoplasmic and Nuclear; C065526:ribosome receptor; C000602809:RRBP1 protein, human; D002352:Carrier Proteins