title: Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061.
abstract: The four overlapping cosmids from the rubradirin producer, Streptomyces achromogenes var rubradiris NRRL 3061, have 58 ORFs within a 105.6 kb fragment. These ORFs harbored essential genes responsible for the formation and attachment of four distinct moieties, along with the genes associated with regulatory, resistance, and transport functions. The PKS (rubA) and glycosyltransferase (rubG2) genes were disrupted in order to demonstrate a complete elimination of rubradirin production. The rubradirin biosynthetic pathway was proposed based on the putative functions of the gene products, the functional identification of sugar genes, and the mutant strains.
authors: Department of Pharmaceutical Engineering, Inje University, Kimhae, Kyung-nam, Republic of Korea.
authors: Chun-Gyu
authors: CG
authors: Kim
authors: Janardan
authors: J
authors: Lamichhane
authors: Kwang-Il
authors: KI
authors: Song
authors: Van D
authors: VD
authors: Nguyen
authors: Dong-Hee
authors: DH
authors: Kim
authors: Tae-Sung
authors: TS
authors: Jeong
authors: Sung-Ho
authors: SH
authors: Kang
authors: Kyoung-Wook
authors: KW
authors: Kim
authors: Jyoti
authors: J
authors: Maharjan
authors: Young-Soo
authors: YS
authors: Hong
authors: Jae Seon
authors: JS
authors: Kang
authors: Jin-Cheol
authors: JC
authors: Yoo
authors: Jung-Joon
authors: JJ
authors: Lee
authors: Tae-Jin
authors: TJ
authors: Oh
authors: Kwangkyoung
authors: K
authors: Liou
authors: Jae Kyung
authors: JK
authors: Sohng
mesh_terms: D062365:Aminobenzoates / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001483:Base Sequence; D003360:Cosmids; D004269:DNA, Bacterial / Q000032:analysis; D005285:Fermentation; D020868:Gene Silencing; D006027:Glycosides / Q000096:biosynthesis; D062385:Hydroxybenzoates; D008969:Molecular Sequence Data; D005810:Multigene Family; D009285:Naphthoquinones / Q000378:metabolism; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
chemical_list: D062365:Aminobenzoates; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D006027:Glycosides; D062385:Hydroxybenzoates; D009285:Naphthoquinones; C024513:rubranitrose; C031509:3-amino-5-hydroxybenzoic acid; C002857:rubradirin