title: Histocompatible chicken inbred lines: homogeneities in the major histocompatibility complex antigens of the GSP, GSN/1, PNP/DO and BM-C inbred lines assessed by hemagglutination, mixed lymphocyte reaction and skin transplantation.
abstract: Chicken inbred lines of the GSP, GSN/1, PNP/DO and BM-C have been established by selection of a specific allele at the B blood group locus (MHC B-G region) and other polymorphic loci through pedigree mating. To extend the potential of these inbred lines as experimental animals in Aves, we assessed the antigenic homogeneities of the MHC antigens by three immunological methods. Antigenic variations of red blood cells (RBCs) were surveyed in the inbred lines and a random-bred line (NG) derived from the Nagoya breed by using ten kinds of intact antisera produced in the inbred line of chickens against RBCs of a red junglefowl and hybrids. In the hemagglutination test, no individual variations were found within the inbred line at all, while all the ten antisera detected highly heterogeneous reactions in individuals of the NG. The reciprocal one-way mixed lymphocyte reactions gave constantly higher stimulation responses (P<0.01) between individual pairs from the inbred lines having different B alleles compared to pairs within the inbred line, while lower stimulation was observed between pairs of the GSP and GSN/1 inbred lines both having the B(21) allele. In reciprocal skin transplantation, the transplanted skingrafts within the inbred line and between individuals from the GSP and GSN/1 inbred lines survived more than 100 days, while all the skingrafts showed signs of rejection within 7 days among the inbred lines having different B alleles. The results obtained by the three practical methods coincidentally indicated that the individuals in the respective four inbred lines were histocompatible, and further, that the GSP and GSN/1 individuals were histocompatible.
authors: Laboratory of Animal Genetics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Japan.
authors: Marcos B
authors: MB
authors: Valdez
authors: Makoto
authors: M
authors: Mizutani
authors: Akira
authors: A
authors: Fujiwara
authors: Hajime
authors: H
authors: Yazawa
authors: Takahiro
authors: T
authors: Yamagata
authors: Kiyoshi
authors: K
authors: Shimada
authors: Takao
authors: T
authors: Namikawa
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics*; D005260:Female; D006386:Hemagglutination Tests; D006648:Histocompatibility; D006649:Histocompatibility Antigens / Q000235:genetics*; D007178:Inbreeding; D007959:Lymphocyte Culture Test, Mixed; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008297:Male; D016038:Skin Transplantation
chemical_list: D006649:Histocompatibility Antigens