title: Synthesis of pyrene labeled RNA for fluorescence measurements.
abstract: The fluorophores 1-ethynylpyrene and 1-(p-ethynyl-phenylethynyl)-pyrene were attached to RNA through a Sonogashira cross-coupling with 5-iodocytidine either in solution through phosphoamidite synthesis or via on-column conjugation during solid-phase oligonucleotide synthesis. Six probes with the sequence 5'-CUU UUC UUU CUU-3' were derivatized with both fluorophores, whereby the position of the modified cytidine was varied. Fluorescence measurements showed sensitivity of the pyrene group to its environment in the single strands and corresponding duplexes.
authors: Institut für Organische Chemie und Chemische Biologie (OCCB), Johann Wolfgang Goethe-Universität, Max-von-Laue Strasse 7, D-60438 Frankfurt am Main, Germany.
authors: Taewoo
authors: T
authors: Kwon
authors: Nelly
authors: N
authors: Piton
authors: Christian
authors: C
authors: Grünewald
authors: Joachim W
authors: JW
authors: Engels
mesh_terms: D005453:Fluorescence; D009841:Oligonucleotides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D015347:RNA Probes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: C527050:1-(p-ethynyl-phenylethynyl)-pyrene; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D011721:Pyrenes; D015347:RNA Probes; C434331:phosphoramidite; C042881:1-ethynylpyrene; D012313:RNA