title: Fast and slow myosins as markers of muscle injury.
abstract: OBJECTIVE
  The diagnosis of muscular lesions suffered by athletes is usually made by clinical criteria combined with imaging of the lesion (ultrasonography and/or magnetic resonance) and blood tests to detect the presence of non-specific muscle markers. This study was undertaken to evaluate injury to fast and slow-twitch fibres using specific muscle markers for these fibres.
  
  
  METHODS
  Blood samples were obtained from 51 non-sports people and 38 sportsmen with skeletal muscle injury. Western blood analysis was performed to determine fast and slow myosin and creatine kinase (CK) levels. Skeletal muscle damage was diagnosed by physical examination, ultrasonography and magnetic resonance and biochemical markers.
  
  
  RESULTS
  The imaging tests were found to be excellent for detecting and confirming grade II and III lesions. However, grade I lesions were often unconfirmed by these techniques. Grade I lesions have higher levels of fast myosin than slow myosin with a very small increase in CK levels. Grade II and III lesions have high values of both fast and slow myosin.
  
  
  CONCLUSIONS
  The evaluation of fast and slow myosin in the blood 48 h after the lesion occurs is a useful aid for the detection of type I lesions in particular, since fast myosin is an exclusive skeletal muscle marker. The correct diagnosis of grade I lesions can prevent progression of the injury in athletes undergoing continual training sessions and competitions, thus aiding sports physicians in their decision making.
authors: University of Barcelona, Barcelona, Spain.
authors: M
authors: M
authors: Guerrero
authors: M
authors: M
authors: Guiu-Comadevall
authors: J A
authors: JA
authors: Cadefau
authors: J
authors: J
authors: Parra
authors: R
authors: R
authors: Balius
authors: A
authors: A
authors: Estruch
authors: G
authors: G
authors: Rodas
authors: J L
authors: JL
authors: Bedini
authors: R
authors: R
authors: Cussó
mesh_terms: D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D001265:Athletic Injuries / Q000000981:diagnostic imaging / Q000517:prevention & control*; D015415:Biomarkers / Q000097:blood; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D018656:Muscle Fibers, Fast-Twitch / Q000000981:diagnostic imaging / Q000378:metabolism*; D018657:Muscle Fibers, Slow-Twitch / Q000000981:diagnostic imaging / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000000981:diagnostic imaging / Q000293:injuries*; D009218:Myosins / Q000097:blood*; D014463:Ultrasonography
chemical_list: D015415:Biomarkers; D009218:Myosins