Optimisation of wort volatile analysis by headspace solid-phase microextraction in combination with gas chromatography and mass spectrometry.
The aim of this study was to create a simple, solventless technique without derivatisation in order to analyze a broad range of volatiles in beer wort. A method was developed using headspace solid-phase microextraction coupled with gas chromatography and mass spectrometry. The procedure was optimised by selection of the appropriate fibre and optimisation of extraction temperature, extraction time, and salting-out. The detection limits were well below the actual wort concentrations of the selected volatiles, ranging from 12 ng/l for linalool to 0.53 microg/l for furfural. Moreover, the procedure showed a good linearity and was applied to the analysis of wort samples taken from a wort boiling process in an industrial brewery.
De Schutter
David P
DP
Centre for Malting and Brewing Science, Faculty of Bioscience Engineering, K.U. Leuven, Kasteelpark Arenberg 22, Box 2463, 3001 Heverlee, Belgium. david.deschutter@biw.kuleuven.be
Saison
Daan
D
Delvaux
Filip
F
Derdelinckx
Guy
G
Rock
Jean-Marie
JM
Neven
Hedwig
H
Delvaux
Freddy R
FR
D000447:Aldehydes / Q000032:analysis; D001515:Beer / Q000032:analysis*; D005421:Flavoring Agents / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*; D013696:Temperature; D014835:Volatilization
D000447:Aldehydes; D005421:Flavoring Agents