In vitro anticholinesterase activity of various alkaloids.
In the current study, a number of alkaloids including retamine, cytisine, and sparteine (quinolizidine-type), yohimbine and vincamine (indole-type), scopolamine and atropine (tropane-type), colchicine (tropolone-type), allantoin (imidazolidine-type), trigonelline (pyridine-type) as well as octopamine, synephrine, and capsaicin (exocyclic amine-type) were tested in vitro for their inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) at 1 mg/ml concentration by the Ellman method using an ELISA microplate reader. Among the alkaloids tested, only capsaicin exerted a remarkable inhibitory effect towards both AChE and BChE [(62.7 +/- 0.79)% and (75.3 +/- 0.98)%, respectively]. While the rest of the alkaloids did not show any significant inhibition against AChE, three of the alkaloids, namely retamine, sparteine, and yohimbine, exerted a noteworthy anti-BChE effect as compared to galanthamine, the reference drug.
Orhan
Ilkay
I
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey. iorhan@gazi.edu.tr
Naz
Qamar
Q
Kartal
Murat
M
Tosun
Fatma
F
Sener
Bilge
B
Choudhary
M Iqbal
MI
D000110:Acetylcholinesterase / Q000187:drug effects / Q000378:metabolism; D000470:Alkaloids / Q000145:classification / Q000494:pharmacology*; D002091:Butyrylcholinesterase / Q000187:drug effects / Q000378:metabolism; D002211:Capsaicin / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D007700:Kinetics; D009655:Octopamine / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013578:Synephrine / Q000494:pharmacology
D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D009655:Octopamine; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D013578:Synephrine; D002211:Capsaicin