title: Usefulness of a quick decalcification of bone sections embedded in methyl methacrylate[corrected]: an improved method for immunohistochemistry.
abstract: Immunohistochemistry of undecalcified bone sections embedded in methyl methacrylate (MMA) is not commonly employed because of potential destruction of tissue antigenicity by highly exothermic polymerization. The aim of the present study was to describe a new technique in which a quick decalcification of bone sections embedded in MMA improves the results for immunohistochemistry. The quality of interleukin 1alpha (IL-1alpha) immunostaining according to the present method was better than the conventional one. Immunostaining for osteoprotegerin (OPG) and the receptor activator of NF-kappaB ligand (RANKL) in bone sections of chronic kidney disease patients with mineral bone disorders (CKD-MBD) was stronger than in controls (postmortem healthy subjects). The present study suggested that this method is easy, fast, and effective to perform both histomorphometry and immunohistochemistry in the same bone fragment, yielding new insights into pathophysiological aspects and therapeutic approaches in bone disease.
authors: Division of Nephrology, Universidade Federal de São Paulo, Rua Botucatu, 740 Vila Clementino, São Paulo, SP Brazil 04023-062. samirah@nefro.epm.br
authors: Samirah Abreu
authors: SA
authors: Gomes
authors: Luciene Machado
authors: LM
authors: dos Reis
authors: Ivone Braga
authors: IB
authors: de Oliveira
authors: Irene de Lourdes
authors: IL
authors: Noronha
authors: Vanda
authors: V
authors: Jorgetti
authors: Ita Pfeferman
authors: IP
authors: Heilberg
mesh_terms: D001847:Bone Diseases / Q000473:pathology; D001842:Bone and Bones / Q000166:cytology*; D003648:Decalcification Technique / Q000379:methods*; D006801:Humans; D007085:Ilium / Q000473:pathology; D007150:Immunohistochemistry / Q000379:methods*; D007676:Kidney Failure, Chronic / Q000473:pathology; D020366:Methylmethacrylate; D016610:Tissue Embedding / Q000379:methods
chemical_list: D020366:Methylmethacrylate