title: Study of UVA irradiation on hemoglobin in the presence of NADH.
abstract: Reduction of ferric methemoglobin (metHb) to its ferrous form is observed by short-time ultraviolet A (UVA) irradiation of metHb together with nicotinamide adenine dinucleotide (NADH). And, severely structural destruction of metHb occurs when long-time UVA irradiation is exerted. However, neither reduction nor destruction can be observed in the absence of NADH under otherwise the same experimental conditions. Accordingly, the O2-binding ability of the protein increases by short-time UVA irradiation of metHb together with NADH, which corresponds with the reduction of metHb, while it decreases by long-time UVA irradiation, which corresponds with the structural destruction. Besides, it is found that the reduction reaction and the conformational destruction proceed more rapidly with higher concentrations of NADH.
authors: School of Life Science and Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University, Shanghai 200444, PR China.
authors: Xiaoqiang
authors: X
authors: Liang
authors: Guifang
authors: G
authors: Chen
authors: Xin
authors: X
authors: Zhang
authors: Shanli
authors: S
authors: Liu
authors: Genxi
authors: G
authors: Li
mesh_terms: D006454:Hemoglobins / Q000737:chemistry* / Q000528:radiation effects*; D006801:Humans; D009243:NAD / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry; D013053:Spectrophotometry; D014466:Ultraviolet Rays
chemical_list: D006454:Hemoglobins; D009243:NAD; D010100:Oxygen