2'-O-hydroxyalkoxymethylribonucleosides and their incorporation into oligoribonucleotides.
A simple and efficient method for the preparation of pyrimidine 2'-O-hydroxyethoxymethylribonucleosides and 2'-O-hydroxypropoxymethylribonucleosides has been developed. These modified nucleosides were incorporated into oligoribonucleotides, which were shown to form stable RNA/RNA duplexes. The effect of 2' -O-modification in the antisense and sense strands of small interference RNA was evaluated in multi-drug resistant NIH 3T3 cells.
Mikhailov
Sergey N
SN
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Bobkov
Georgii V
GV
Brilliantov
Kyrill V
KV
Rozenski
Jef
J
Van Aerschot
Arthur
A
Herdewijn
Piet
P
Fisher
Michael H
MH
Juliano
Rudolph L
RL
D009843:Oligoribonucleotides / Q000737:chemistry*; D011743:Pyrimidines / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D012263:Ribonucleosides / Q000138:chemical synthesis* / Q000737:chemistry
D009843:Oligoribonucleotides; D011743:Pyrimidines; D012263:Ribonucleosides; D012313:RNA; C030986:pyrimidine