title: Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster.
abstract: The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.
authors: Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02175, USA.
authors: Jian-Quan
authors: JQ
authors: Ni
authors: Michele
authors: M
authors: Markstein
authors: Richard
authors: R
authors: Binari
authors: Barret
authors: B
authors: Pfeiffer
authors: Lu-Ping
authors: LP
authors: Liu
authors: Christians
authors: C
authors: Villalta
authors: Matthew
authors: M
authors: Booker
authors: Lizabeth
authors: L
authors: Perkins
authors: Norbert
authors: N
authors: Perrimon
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D034622:RNA Interference
chemical_list: D029721:Drosophila Proteins