Multidimensional chromatography: validation and efficient fishing for biomarkers and fractions containing them using the VisualCockpit software package.
2-D native LC yields thousands of fractions especially when applied to sera of different origin. Checking reproducibility of repeated separation of the same serum or searching for biomarker candidates and fractions containing them requires finding, selection, and comparison of interesting data subsets out of huge data volumes. An innovative software package is applied that markedly enhances simplicity, velocity, and reliability of (i) check of reproducibility of the separation method and (ii) analysis of proteomes pertaining to different disease states.
Cumme
Gerhard A
GA
Institute of Biochemistry I, Medical Faculty, Friedrich Schiller University, Jena, Germany.
Kreusch
Stefan
S
Nagel
Matthias
M
Rhode
Heidrun
H
D015415:Biomarkers / Q000032:analysis* / Q000097:blood; D005591:Chemical Fractionation; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid / Q000379:methods; D019295:Computational Biology; D006801:Humans; D020543:Proteome / Q000032:analysis / Q000737:chemistry; D044967:Serum / Q000737:chemistry; D012984:Software; D013056:Spectrophotometry, Ultraviolet
D015415:Biomarkers; D020543:Proteome