title: Optimization of culture conditions for production of cis-epoxysuccinic acid hydrolase using response surface methodology.
abstract: Response surface methodology, which allows for rapid identification of important factors and optimization of them to enhance enzyme production, was employed here to optimize culture conditions for the production of cis-epoxysuccinic acid hydrolase from Bordetella sp. strain 1-3. In the first step, a Plackett-Burman design was used to evaluate the effects of nine variables (yeast extract, cis-epoxysuccinic acid, KH(2)PO(4), K(2)HPO(4).3H(2)O, MgSO(4).7H(2)O, trace minerals solution, culture volume, initial pH and incubation time) on the enzyme production. Yeast extract, cis-epoxysuccinic acid and KH(2)PO(4) had significant influences on cis-epoxysuccinic acid hydrolase production and their concentrations were further optimized using central composite design and response surface analysis. A combination of adjusting the concentration of yeast extract to 7.8 g/l, cis-epoxysuccinic acid to 9.8 g/l, and KH(2)PO(4) to 1.12 g/l would favor maximum cis-epoxysuccinic acid hydrolase production. An enhancement of cis-epoxysuccinic acid hydrolase production from 5.6 U/ml to 9.27 U/ml was gained after optimization.
authors: College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors: Xia
authors: X
authors: Li
authors: Tongcheng
authors: T
authors: Xu
authors: Xiaohang
authors: X
authors: Ma
authors: Kangping
authors: K
authors: Guo
authors: Lei
authors: L
authors: Kai
authors: Yuhua
authors: Y
authors: Zhao
authors: Xiaoming
authors: X
authors: Jia
authors: Yin
authors: Y
authors: Ma
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D001884:Bordetella / Q000201:enzymology*; D004851:Epoxide Hydrolases / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism*; D005285:Fermentation; D007700:Kinetics; D008322:Mammals; D019802:Succinic Acid / Q000378:metabolism*; D013499:Surface Properties; D013644:Tartrates / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004852:Epoxy Compounds; D013644:Tartrates; D019802:Succinic Acid; D004851:Epoxide Hydrolases; C029768:tartaric acid