title: A luminescent sensor for tyrosine phosphorylation.
abstract: We have developed a luminogenic probe for tyrosine phosphorylation based on a short peptide sequence containing an iminodiacetate moiety near the site of phosphorylation. In response to kinase activity, the probe provides a strong luminescence enhancement, resulting from the increased ability of the probe to bind and sensitize Tb3+ and Eu3+ ions upon phosphorylation.
authors: Department of Chemistry, Columbia University, New York, New York 10027, USA.
authors: Matthew S
authors: MS
authors: Tremblay
authors: Minhee
authors: M
authors: Lee
authors: Dalibor
authors: D
authors: Sames
mesh_terms: D000595:Amino Acid Sequence; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008674:Metals, Rare Earth; D015394:Molecular Structure; D010766:Phosphorylation; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000378:metabolism; D013725:Terbium / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry*; D019061:src-Family Kinases / Q000378:metabolism
chemical_list: D028581:Lanthanoid Series Elements; D008674:Metals, Rare Earth; D013725:Terbium; D014443:Tyrosine; D019061:src-Family Kinases; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1