[Expression of GATA-1 and GATA-2 in the bone marrow of patients with Monge's disease].
OBJECTIVE
To find out how GATA-1 and GATA-2 behave in the bone marrow of patients with Monge's disease.


METHODS
The levels of mRNA in mononuclear cells (MNC) and proteins of GATA-1 and GATA-2 in the bone marrow of patients with Monge's disease and controls were determined by RT-PCR and immune cytolysis chemical method.


RESULTS
(1) All patients and controls expressed GATA-1 mRNA (Monge's disease 1.033 +/- 0.146, Control 0.458 +/- 0.076) and GATA-2 mRNA (Monge's disease 0.451 +/- 0.073, Control 0.185 +/- 0.074). All patients expressed both GATA-1 (positive cell counts 77.3 +/- 33.3, positive score 135.4 +/- 75.4) and GATA-2 ( positive cell counts 29.4 +/- 11.4, positive score 48.4 +/- 19.7). All the controls expressed GATA-1 (positive cell counts 18.1 +/- 11.3, positive score 24.2 +/- 13.4) while 12 of 20 controls expressed GATA-2 ( positive cell counts 5.4 +/- 3.0, positive score 7.3 +/- 4.2). The expression of mRNA and proteins of GATA-1 and GATA-2 in Monge's disease were higher than in controls (P < 0.01). (2) There was a positive correlation between GATA-1 and Hb (P < 0.01), as did between mRNA and proteins of GATA-1 and GATA-2. (3) Both the proteins of GATA-1 and GATA-2 located only in the cytoplasm but not the nucleus.


CONCLUSIONS
Two of inherent genes, GATA-1 and GATA-2 which were expressed at higher levels in patients with Monge's disease than in controls might play significant roles in the pathogenesis of Monge's disease.
Li
Jian-ping
JP
Department of Hematology, Affiliated Hospital of Qinghai University, Xining, China.
Jia
Nai-yong
NY
Li
Zhan-quan
ZQ
Wang
Hong-xin
HX
Su
Juan
J
Li
Yu-li
YL
Li
Xue-mei
XM
D000328:Adult; D000532:Altitude Sickness / Q000378:metabolism*; D050982:GATA1 Transcription Factor / Q000378:metabolism*; D050989:GATA2 Transcription Factor / Q000378:metabolism*; D006801:Humans; D008297:Male; D011086:Polycythemia / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
D050982:GATA1 Transcription Factor; C494688:GATA1 protein, human; D050989:GATA2 Transcription Factor; D012333:RNA, Messenger