Rapid determination of acetaminophen and p-aminophenol in pharmaceutical formulations using miniaturized capillary electrophoresis with amperometric detection.
Capability of fast analysis of a novel miniaturized capillary electrophoresis with carbon disk electrode amperometric detection (mini-CE-AD) system was demonstrated by determining acetaminophen and p-aminophenol in dosage forms. Factors influencing the separation and detection processes were examined and optimized. Under the optimum conditions, the end-capillary 300 microm carbon disc electrode amperometric detector offered favorable signal-to-noise characteristics at a relatively low potential (+600 mV versus Ag/AgCl) for detecting acetaminophen and p-aminophenol. Two analytes can been separated within 150 s in a 8.5 cm length capillary at a separation voltage of 2000V using a Na2B4O7-KH2PO4 running buffer (pH 7.2). Acetaminophen and p-aminophenol could be detected down to the 1.4 x 10(-6)-5.9 x 10(-7) molL(-1) level with linearity up to the 1.0 x 10(-3) molL(-1) level examined. The inter-day repeatability for analytes in peak current (R.S.D.< or =2.3%) and migration times (R.S.D.< or =1.3%) were excellent. The proposed mini-CE-AD system should find a wide range of analytical applications in pharmaceutical formulations as an alternative to conventional CE and mu-CE.
Chu
Qingcui
Q
Department of Chemistry, East China Normal University, 3663 North Zhongshan Road, Shanghai 200062, PR China.
Jiang
Lianmei
L
Tian
Xiuhui
X
Ye
Jiannong
J
D000082:Acetaminophen / Q000032:analysis*; D000627:Aminophenols / Q000032:analysis*; D018712:Analgesics, Non-Narcotic / Q000032:analysis; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D008904:Miniaturization; D015394:Molecular Structure; D009153:Mutagens / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D013997:Time Factors
D000627:Aminophenols; D018712:Analgesics, Non-Narcotic; D009153:Mutagens; D004364:Pharmaceutical Preparations; D000082:Acetaminophen; C026729:4-aminophenol