title: Survival and fertility rate of cooled dromedary camel spermatozoa supplemented with catalase enzyme.
abstract: The present study was planned to study the effects of addition of different concentrations of catalase enzyme (0, 250, 500 and 1,000 IU/ml) to cooled dromedary camel semen extended with tris-yolk-fructose extender on semen quality during storage at 5 C for up to 5 days. Conception rates of she-camels artificially inseminated with whole fresh or extended cooled dromedary camel semen with or without 500 IU/ml catalase enzyme were also estimated. The results showed that addition of catalase enzyme at concentrations of 250 or 500 IU/ml to extended cooled dromedary camel semen significantly increased (P<0.01) the percentage of sperm motility and significantly decreased (P<0.01) the percentages of dead spermatozoa, sperm abnormalities and acrosomal damage. The highest (P<0.01) percentage of sperm motility was recorded with extended cooled dromedary camel semen supplemented with catalase enzyme at a concentration of 500 IU/ml, and the lowest (P<0.01) value was recorded with catalase enzyme at a concentration of 1000 IU/ml. On the other hand, the lowest (P<0.01) percentages of dead spermatozoa, sperm abnormalities and acrosomal damage of spermatozoa were recorded with extended cooled dromedary camel semen supplemented with 500 IU/ml, and the highest (P<0.01) values were recorded with catalase enzyme at a concentration of 1,000 IU/ml. Advancement of the storage time at 5 C significantly decreased (P<0.01) the percentage of sperm motility and significantly increased (P<0.01) the percentages of dead spermatozoa, sperm abnormalities and acrosomal damage of spermatozoa. Moreover, the conception rates of she-camels artificially inseminated with whole fresh, extended cooled dromedary camel semen free-catalase enzyme and extended cooled dromedary camel semen supplemented with catalase enzyme at a concentration of 500 IU/ml were 46.15, 22.22 and 37.50%, respectively. In conclusion, the results show that addition of catalase enzyme at a concentration of 500 IU/ml to semen extender can be used as an agent for prolongation of dromedary camel sperm cell survival during storage at 5 C.
authors: Department of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt. medan69@hotmail.com
authors: Mohamed Sabry
authors: MS
authors: Medan
authors: Gamal
authors: G
authors: Absy
authors: Alaa Elsayed
authors: AE
authors: Zeidan
authors: Medhat Hussein
authors: MH
authors: Khalil
authors: Hesham Hussein
authors: HH
authors: Khalifa
authors: Atef Mahrous
authors: AM
authors: Abdel-Salaam
authors: Tarek Mohammad
authors: TM
authors: Abdel-Khalek
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002162:Camelus; D002374:Catalase / Q000494:pharmacology*; D003080:Cold Temperature; D005260:Female; D005306:Fertilization / Q000187:drug effects*; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D012661:Semen / Q000166:cytology; D012662:Semen Preservation / Q000662:veterinary*; D013094:Spermatozoa / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D002374:Catalase