title: Distinct motifs of neuropeptide Y receptors differentially regulate trafficking and desensitization.
abstract: Activated human neuropeptide Y Y(1) receptors rapidly desensitize and internalize through clathrin-coated pits and recycle from early and recycling endosomes, unlike Y(2) receptors that neither internalize nor desensitize. To identify motifs implicated in Y(1) receptor desensitization and trafficking, mutants with varying C-terminal truncations or a substituted Y(2) C-terminus were constructed. Point mutations of key putative residues were made in a C-terminal conserved motif [phi-H-(S/T)-(E/D)-V-(S/T)-X-T] that we have identified and in the second intracellular i2 loop. Receptors were analyzed by functional assays, spectrofluorimetric measurements on living cells, flow cytometry, confocal imaging and bioluminescence resonance energy transfer assays for beta-arrestin activation and adaptor protein (AP-2) complex recruitment. Inhibitory GTP-binding protein-dependent signaling of Y(1) receptors to adenylyl cyclase and desensitization was unaffected by C-terminal truncations or mutations, while C-terminal deletion mutants of 42 and 61 amino acids no longer internalized. Substitutions of Thr357, Asp358, Ser360 and Thr362 by Ala in the C-terminus abolished both internalization and beta-arrestin activation but not desensitization. A Pro145 substitution by His in an i2 consensus motif reported to mediate phosphorylation-independent recruitment of beta-arrestins affected neither desensitization, internalization or recycling kinetics of activated Y(1) receptors nor beta-arrestin activation. Interestingly, combining Pro145 substitution by His and C-terminal substitutions significantly attenuates Y(1) desensitization. In the Y(2) receptor, replacement of His155 with Pro at this position in the i2 loop motif promotes agonist-mediated desensitization, beta-arrestin activation, internalization and recycling. Overall, our results indicate that beta-arrestin-mediated desensitization and internalization of Y(1) and Y(2) receptors are differentially regulated by the C-terminal motif and the i2 loop consensus motif.
authors: Institut Gilbert-Laustriat, UMR 7175, CNRS/Université Louis Pasteur, Strasbourg I, France.
authors: Moussa
authors: M
authors: Ouedraogo
authors: Sandra
authors: S
authors: Lecat
authors: Moulay Driss
authors: MD
authors: Rochdi
authors: Muriel
authors: M
authors: Hachet-Haas
authors: Hans
authors: H
authors: Matthes
authors: Hervé
authors: H
authors: Gicquiaux
authors: Sophie
authors: S
authors: Verrier
authors: Mireille
authors: M
authors: Gaire
authors: Nicole
authors: N
authors: Glasser
authors: Yves
authors: Y
authors: Mély
authors: Kenneth
authors: K
authors: Takeda
authors: Michel
authors: M
authors: Bouvier
authors: Jean-Luc
authors: JL
authors: Galzi
authors: Bernard
authors: B
authors: Bucher
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D019390:Arrestins / Q000378:metabolism; D001693:Biological Transport, Active; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D017476:Receptors, Neuropeptide Y / Q000819:agonists / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D000071557:beta-Arrestins
chemical_list: D019390:Arrestins; D017476:Receptors, Neuropeptide Y; D011993:Recombinant Fusion Proteins; D000071557:beta-Arrestins; C485184:enhanced green fluorescent protein; C097212:neuropeptide Y-Y1 receptor; C095889:neuropeptide Y2 receptor; D049452:Green Fluorescent Proteins; D000242:Cyclic AMP; D000262:Adenylyl Cyclases