title: Combined scanning optical coherence and two-photon-excited fluorescence microscopy.
abstract: We demonstrate simultaneous imaging by optical coherence microscopy (OCM) and two-photon-excited (TPE) fluorescence microscopy. A mode-locked Ti:sapphire laser is focused and scanned in three dimensions through a fixed sample, generating both backscattered light and fluorescence light, which are independently detected. Both imaging modes provide rapid en-face imaging with submicrometer resolution. High-power delivery into the sample yields an OCM sensitivity in excess of 130 dB at 100-kHz pixel rates. Simultaneous imaging of cell nuclei with OCM and TPE is demonstrated in live drosophila embryos.
authors: E
authors: E
authors: Beaurepaire
authors: L
authors: L
authors: Moreaux
authors: F
authors: F
authors: Amblard
authors: J
authors: J
authors: Mertz