Structural characterization of the O-chain polysaccharide of Aeromonas caviae ATCC 15468 lipopolysaccharide.
The O-chain polysaccharide produced by a mild acid degradation of Aeromonas caviae ATCC 15468 lipopolysaccharide was found to be composed of L-rhamnose, 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose and phosphoglycerol. Subsequent methylation and CE-ESIMS analyses and 1D/2D NMR ((1)H, (13)C and (31)P) spectroscopy showed that the O-chain polysaccharide is a high-molecular-mass acidic branched polymer of tetrasaccharide repeating units with a phosphoglycerol substituent having the following structure: [structure: see text] where Gro represents glycerol and P represents a phosphate group.
Wang
Zhan
Z
Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada.
Liu
Xin
X
Li
Jianjun
J
Altman
Eleonora
E
D000333:Aeromonas / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005690:Galactose; D005947:Glucose; D005994:Glycerophosphates; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D009844:Oligosaccharides; D012210:Rhamnose
D005994:Glycerophosphates; D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides; D005947:Glucose; D012210:Rhamnose; D005690:Galactose