title: Up to 151 days of continuous animal perfusion with trivial heparin infusion by the application of a long-term durable antithrombogenic coating to a combination of a seal-less centrifugal pump and a diffusion membrane oxygenator.
abstract: We developed a new coating material (Toyobo-National Cardiovascular Center coating) for medical devices that delivers high antithrombogenicity and long-term durability. We applied this coating to an extracorporeal membrane oxygenation (ECMO) system, including the circuit tube, cannulae, a seal-less centrifugal pump, and a diffusion membrane oxygenator, to realize prolonged cardiopulmonary support with trivial anticoagulant infusion. The oxygenator consisted of a hollow-fiber membrane made of polymethylpentene, which allows the transfer of gas by diffusion through the membrane. The centrifugal pump was free of seals and had a pivot bearing. We performed a venoarterial bypass in a goat using this ECMO system, and the system was driven for 151 days with trivial anticoagulant infusion. Plasma leakage from the oxygenator did not occur and sufficient gas-exchange performance was well maintained. In the oxygenator, thrombus formation was present around the top and the distributor of the inlet portion and was very slight in the outlet portion. In the centrifugal blood pump, there was some wear in the female pivot region and quite small amounts of thrombus formation on the edge of the shroud; the pivot wear seemed to be the cause of the hemolysis observed after 20 weeks of perfusion and which resulted in the termination of the perfusion. However, no significant amounts of thrombus were observed in other parts of the system. This ECMO system showed potential for long-term cardiopulmonary support with minimal use of systemic anticoagulants.
authors: Department of Artificial Organs, National Cardiovascular Center, Research Institute, 5-7-1 Fujishirodai, Suita 565-8565, Japan. nishinak@ri.ncvc.go.jp
authors: Tomohiro
authors: T
authors: Nishinaka
authors: Eisuke
authors: E
authors: Tatsumi
authors: Nobumasa
authors: N
authors: Katagiri
authors: Hiroyuki
authors: H
authors: Ohnishi
authors: Toshihide
authors: T
authors: Mizuno
authors: Kyoko
authors: K
authors: Shioya
authors: Tomonori
authors: T
authors: Tsukiya
authors: Akihiko
authors: A
authors: Homma
authors: Susumu
authors: S
authors: Kashiwabara
authors: Hidenori
authors: H
authors: Tanaka
authors: Masaki
authors: M
authors: Sato
authors: Yoshiyuki
authors: Y
authors: Taenaka
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000008:administration & dosage*; D002498:Centrifugation; D020099:Coated Materials, Biocompatible; D004195:Disease Models, Animal; D004867:Equipment Design; D015199:Extracorporeal Membrane Oxygenation / Q000295:instrumentation*; D006041:Goats; D006493:Heparin / Q000008:administration & dosage*; D010477:Perfusion / Q000295:instrumentation*; D013997:Time Factors
chemical_list: D000925:Anticoagulants; D020099:Coated Materials, Biocompatible; D006493:Heparin