title: Semi-automated microseeding of nanolitre crystallization experiments.
abstract: A simple semi-automated microseeding procedure for nanolitre crystallization experiments is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96-well crystallization plates, facilitating the incorporation of seeding into high-throughput crystallization pipelines. This basic microseeding procedure has been modified to include additive-screening and cross-seeding methods. Five examples in which these techniques have been used successfully are described.
authors: Oxford Protein Production Facility and Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, England.
authors: Thomas S
authors: TS
authors: Walter
authors: Erika J
authors: EJ
authors: Mancini
authors: Jan
authors: J
authors: Kadlec
authors: Stephen C
authors: SC
authors: Graham
authors: René
authors: R
authors: Assenberg
authors: Jingshan
authors: J
authors: Ren
authors: Sarah
authors: S
authors: Sainsbury
authors: Raymond J
authors: RJ
authors: Owens
authors: David I
authors: DI
authors: Stuart
authors: Jonathan M
authors: JM
authors: Grimes
authors: Karl
authors: K
authors: Harlos
mesh_terms: D001331:Automation; D001426:Bacterial Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray / Q000379:methods; D029968:Escherichia coli Proteins / Q000737:chemistry; D023281:Genomics; D008958:Models, Molecular; D008968:Molecular Conformation; D036103:Nanotechnology / Q000379:methods*; D040901:Proteomics; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D014764:Viral Proteins