Pyrene-functionalized 2'-amino-alpha-L-LNA as potential diagnostic probes.
Oligodeoxyribonucleotides (ONs) containing two incorporations of 2'-N-(pyren-1-yl)acetyl-2'-amino-alpha-L-LNA monomer Y are sensitive probes for detection of single nucleotide polymorphisms (SNP) in DNA. In addition, the ability of ONs containing pyrene-functionalized 2'-amino-alpha-L-LNA monomers (W-Z) to stabilize duplexes with an abasic site is demonstrated.
Kumar
T Santhosh
TS
Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, 5230 Odense M, Denmark. skt@chem.sdu.dk
Wengel
Jesper
J
Hrdlicka
Patrick J
PJ
D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D009841:Oligonucleotides / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D013938:Thymidine Monophosphate / Q000031:analogs & derivatives* / Q000737:chemistry
C522825:2'-N-(pyren-1-yl)acetyl-2'-amino-alpha-L-LNA; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D013938:Thymidine Monophosphate