A cell line infectible by prion strains from different species.
It has been shown previously that ovine prion protein (PrP(C)) renders rabbit epithelial RK13 cells permissive to the multiplication of ovine prions, thus providing evidence that species barriers can be crossed in cultured cells through the expression of a relevant PrP(C). The present study significantly extended this observation by showing that mouse and bank vole prions can be propagated in RK13 cells that express the corresponding PrP(C). Importantly, the respective molecular patterns of abnormal PrP (PrP(res)) and, where examined, the neuropathological features of the infecting strains appeared to be maintained during the propagation in cell culture. These findings indicate that RK13 cells can be genetically engineered to replicate prion strains faithfully from different species. Such an approach may facilitate investigations of the molecular basis of strain identity and prion diversity.
Courageot
M-P
MP
Virologie et Immunologie Moléculaires, INRA, 78350 Jouy-en-Josas, France.
Daude
N
N
Virologie et Immunologie Moléculaires, INRA, 78350 Jouy-en-Josas, France.
Nonno
R
R
Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy.
Paquet
S
S
Virologie et Immunologie Moléculaires, INRA, 78350 Jouy-en-Josas, France.
Di Bari
M A
MA
Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy.
Le Dur
A
A
Virologie et Immunologie Moléculaires, INRA, 78350 Jouy-en-Josas, France.
Chapuis
J
J
Virologie et Immunologie Moléculaires, INRA, 78350 Jouy-en-Josas, France.
Hill
A F
AF
Department of Biochemistry and Molecular Biology, Bio21, Molecular Science and Biotechnology Institute, University of Melbourne, Victoria, Australia.
Agrimi
U
U
Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy.
Laude
H
H
Virologie et Immunologie Moléculaires, INRA, 78350 Jouy-en-Josas, France.
Vilette
D
D
Virologie et Immunologie Moléculaires, INRA, 78350 Jouy-en-Josas, France.
D000818:Animals; D003411:Arvicolinae; D002460:Cell Line; D007668:Kidney / Q000473:pathology; D051379:Mice; D011328:Prions / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D011817:Rabbits
D011328:Prions