siRNA as a molecular tool for use in Aspergillus niger.
Gene silencing using siRNA has been examined in the industrially-important fungus, Aspergillus niger. Protoplasts of an A. niger strain containing a single genomic copy of the Escherichia coli uidA gene, encoding beta-glucuronidase (GUS), under control of the A. niger glaA promoter at the same genomic locus, were exposed to siRNA targeted against the uidA gene. Down-regulation of uidA mRNA and GUS activity by siRNA was observed in mycelia that developed from the protoplasts. The down-regulation was transient and was not carried over to conidiation. We concluded that gene silencing by siRNA provides a relatively quick method for analysis of gene function in A. niger.
Barnes
Sally E
SE
School of Biology, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.
Alcocer
Marcos J C
MJ
Archer
David B
DB
D001234:Aspergillus niger / Q000235:genetics*; D015536:Down-Regulation; D004926:Escherichia coli / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D005966:Glucuronidase / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011523:Protoplasts / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection; D014994:Xylose / Q000378:metabolism
D034741:RNA, Small Interfering; D008320:Maltose; D014994:Xylose; D005966:Glucuronidase