[Contents mensuration of total alkaloid in Uncaria rhynchophylla by acid dye colorimetry].
OBJECTIVE
To investigate the method of determination of total alkaloids Uncaria rhynchophylla.


METHODS
The Contents of total Alkaloid were determined by Acid dye Colorimetry.


RESULTS
Acid dye color conditions: pH3.6 buffer 5.0 ml, bromocresol green liquid 5.0 ml; chloroform extraction three times, each time was exeracted for 2 minutes, put it aside for at least 5 minutes for the determination of the best method. Rhynchophylline 6.018 microg - 108.324 microg in the linear range, Recoveriys rate was 97.19%, RSD was 1.34% (n = 6).


CONCLUSIONS
The method is simple, highly sensitive and reproducible.
Zeng
Chang-qing
CQ
Guangdong College of Pharmacy, Guangzhou 510006, China.
Luo
Bei-liang
BL
D000470:Alkaloids / Q000032:analysis* / Q000302:isolation & purification / Q000592:standards; D003124:Colorimetry / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012997:Solvents; D032070:Uncaria / Q000737:chemistry*
D000470:Alkaloids; D004396:Coloring Agents; D012997:Solvents