[Effect of oral carcinoma-associated fibroblasts on extracellular signal-regulated kinases pathway in the lingual carcinoma cell line].
OBJECTIVE
To investigate the effects of oral carcinoma-associated fibroblasts (CAFs) on extracellular signal-regulated kinases (ERK) pathway in a lingual carcinoma cell line.


METHODS
The lingual carcinoma cell line, Tca8113 was stimulated by conditioned medium from oral CAFs, or cocultured with oral CAFs for definite time. Total ERK and pERK in Tca8113 lysate were detected by Western blotting, and the ratio between pERK and ERK were calculated.


RESULTS
Both stimulation by conditioned medium and coculture induced prompt phosphorylation of ERK, and increased the ratio between pERK and ERK.


CONCLUSIONS
Oral CAFs can activate ERK pathway of carcinoma cells.
Lin
Jing-wen
JW
Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Chen
Qian-ming
QM
Li
Sheng-fu
SF
Fu
Chun-hua
CH
Lin
Mei
M
Long
Dan
D
Zhou
Hong-mei
HM
D002460:Cell Line; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D048049:Extracellular Signal-Regulated MAP Kinases; D005347:Fibroblasts; D006801:Humans; D009062:Mouth Neoplasms; D010766:Phosphorylation; D014062:Tongue Neoplasms
D048049:Extracellular Signal-Regulated MAP Kinases