title: The nucleoporin Nup358 associates with and regulates interphase microtubules.
abstract: The nucleoporin Nup358 resides on the cytoplasmic face of the interphase nuclear pore complex (NPC). During metaphase, its recruitment to kinetochores is important for correct microtubule-kinetochore attachment. Here, we report that a fraction of endogenous Nup358 interacts with interphase microtubules through its N-terminal region (BPN). Cells overexpressing the microtubule targeting domain of Nup358 displayed dramatic alteration in the microtubule organization including increased microtubule bundling and stability. Ectopic expression of BPN and full-length Nup358 exhibited significantly higher levels of acetylated microtubules that were resistant to nocodazole, a microtubule depolymerizing agent. Furthermore, RNAi mediated depletion of Nup358 affected polarized stabilization of microtubules during directed cell migration, confirming the in vivo role of Nup358 in regulating interphase microtubules.
authors: National Centre for Cell Science, Ganeshkhind, Pune 411 007, India. josephj@nccs.res.in
authors: Jomon
authors: J
authors: Joseph
authors: Mary
authors: M
authors: Dasso
mesh_terms: D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D019556:COS Cells; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D017931:DNA Primers; D007399:Interphase; D008870:Microtubules / Q000502:physiology*; D018832:Molecular Chaperones / Q000502:physiology*; D028861:Nuclear Pore Complex Proteins / Q000502:physiology*; D034622:RNA Interference
chemical_list: D017931:DNA Primers; D018832:Molecular Chaperones; D028861:Nuclear Pore Complex Proteins; C094493:ran-binding protein 2