A novel method for low load friction testing on living cells.
A low load tribology technique for studying the effects of friction on living cells was developed. Results show a direct relationship between the coefficient of friction (COF) and the extent of cell damage. The COF, mu, for a glass pin on an intact layer of human corneal epithelial cells is determined to be on the order of mu = 0.05 +/- 0.02 (n = 16). The correlations between applied normal load and extent of cell damage, as well as between number of reciprocation cycles and cell damage, are reported. It is also found that cell damage can occur when a loading force as low as 0.5 mN is applied, although the cells appear to be intact.
Cobb
Jessica A
JA
J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, P.O. Box 116131, Gainesville, FL, USA.
Dunn
Alison C
AC
Kwon
Jiwoon
J
Sarntinoranont
Malisa
M
Sawyer
W Gregory
WG
Tran-Son-Tay
Roger
R
D002460:Cell Line; D002470:Cell Survival; D004847:Epithelial Cells / Q000502:physiology*; D019573:Epithelium, Corneal / Q000502:physiology*; D017276:Friction; D006801:Humans; D008857:Microscopy, Interference; D013314:Stress, Mechanical