Evaluation of the immunomodulatory activities of royal jelly components in vitro.
In this work the effect of different components isolated from royal jelly (RJ) was studied using an in vitro rat T-cell proliferation assay. We found that lower concentrations of MEL 174 (final water extract of RJ) and MEL 147 (3-10-dihydroxydecanoic acid) stimulated T-cell proliferation, triggered by concanavalin A (Con-A) and the process was followed by an increase in the production of interleukin-2 (IL-2). Higher concentrations of MEL 174, MEL 247 (dry powder of RJ) and MEL 138 (trans-10-hydroxydec-2-enoic acid) inhibited T-cell proliferation. The inhibition of T-cell proliferation in the presence of MEL 174 was followed by a decrease in IL-2 production, which was partly abrogated by exogenous IL-2, a decrease in nitric oxide (NO) production and increased apoptosis. In conclusion, our results showed the complexity of biological activity of RJ and suggest that its water extract possesses the most potent immunomodulatory activity in vitro.
Gasic
Sonja
S
Institute of Medical Research, MMA, Belgrade, Crnotravska, Belgrade, Serbia.
Vucevic
Dragana
D
Vasilijic
Sasa
S
Antunovic
Mirjana
M
Chinou
Ioanna
I
Colic
Miodrag
M
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003208:Concanavalin A; D005227:Fatty Acids / Q000737:chemistry* / Q000494:pharmacology*; D007155:Immunologic Factors; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000032:analysis / Q000378:metabolism; D008297:Male; D000432:Methanol; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000096:biosynthesis; D051381:Rats; D012997:Solvents; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects; D000099219:Royal Jelly
D003208:Concanavalin A; D005227:Fatty Acids; D007155:Immunologic Factors; D007376:Interleukin-2; D000432:Methanol; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide; D012997:Solvents; D000099219:Royal Jelly