Method to determine enantiomeric excess of glucose by nonchiral high-performance liquid chromatography using circular dichroism detection.
Using nonchiral high-performance liquid chromatography (HPLC) coupled with a circular dichroism (CD) detector, we developed a new method to determine the enantiomeric excess (e.e.) of glucose derivatized with chromophores (p-bromobenzoate). This method is suitable for microscale analysis of glycoside and saccharide. As an application of this method, the enantiomeric excess of the component glucose of a glucan was assayed.
Song
Shuang
S
Shenyang Pharmaceutical University, Shenyang 110016, China.
Sun
Lu
L
Yuan
Lei
L
Sun
Tiemin
T
Zhao
Yun
Y
Zuo
Wenjian
W
Cong
Yue
Y
Li
Xian
X
Wang
Jinhui
J
D001970:Bromobenzoates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002942:Circular Dichroism / Q000379:methods*; D005936:Glucans / Q000737:chemistry; D005947:Glucose / Q000031:analogs & derivatives / Q000032:analysis*; D008968:Molecular Conformation; D015203:Reproducibility of Results; D013237:Stereoisomerism
D001970:Bromobenzoates; D005936:Glucans; D005947:Glucose