Thermodynamic evidence of non-muscle myosin II-lipid-membrane interaction.
A unique feature of protein networks in living cells is that they can generate their own force. Proteins such as non-muscle myosin II are an integral part of the cytoskeleton and have the capacity to convert the energy of ATP hydrolysis into directional movement. Non-muscle myosin II can move actin filaments against each other, and depending on the orientation of the filaments and the way in which they are linked together, it can produce contraction, bending, extension, and stiffening. Our measurements with differential scanning calorimetry showed that non-muscle myosin II inserts into negatively charged phospholipid membranes. Using lipid vesicles made of DMPG/DMPC at a molar ratio of 1:1 at 10mg/ml in the presence of different non-muscle myosin II concentrations showed a variation of the main phase transition of the lipid vesicle at around 23 degrees C. With increasing concentrations of non-muscle myosin II the thermotropic properties of the lipid vesicle changed, which is indicative of protein-lipid interaction/insertion. We hypothesize that myosin tail binds to acidic phospholipids through an electrostatic interaction using the basic side groups of positive residues; the flexible, amphipathic helix then may partially penetrate into the bilayer to form an anchor. Using the stopped-flow method, we determined the binding affinity of non-muscle myosin II when anchored to lipid vesicles with actin, which was similar to a pure actin-non-muscle myosin II system. Insertion of myosin tail into the hydrophobic region of lipid membranes, a model known as the lever arm mechanism, might explain how its interaction with actin generates cellular movement.
Schewkunow
Vitali
V
Friedrich-Alexander-University of Erlangen-Nuremberg, Center for Medical Physics and Technology, Biophysics Group, Henkestrasse 91, Erlangen 91052, Germany.
Sharma
Karan P
KP
Diez
Gerold
G
Klemm
Anna H
AH
Sharma
Pal C
PC
Goldmann
Wolfgang H
WH
D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008954:Models, Biological; D008956:Models, Chemical; D015233:Models, Statistical; D020409:Molecular Motor Proteins / Q000737:chemistry*; D009038:Motion; D018482:Muscle, Skeletal / Q000737:chemistry*; D024462:Myosin Type II / Q000737:chemistry*; D013816:Thermodynamics
D008051:Lipid Bilayers; D008081:Liposomes; D020409:Molecular Motor Proteins; D024462:Myosin Type II