[Quantification of Tannerella forsythensis in chronic periodontitis patients].
OBJECTIVE
To compare the number of the Tannerella forsythensis (T. forsythensis), total bacteria, and proportion of T. forsythensis in subgingival specimens in diseased sites of chronic periodontitis patients and in healthy sites of periodontally healthy subjects, and clarify the relationship between bacterial load and periodontal status.


METHODS
Subgingival plaque samples from 61 chronic periodontitis patients and 12 healthy controls (positive for T. forsythensis by conventional PCR) were analyzed with TaqMan real-time polymerase chain reaction assay for T. forsythensis and total bacteria. Quantification was performed with species-specific primer/probe, universal primer/ probe and serial dilution of plasmid standards.


RESULTS
Numbers of T. forsythensis and total bacteria(P<0.001) , the proportion of T. forsythensis in subgingival specimens (P<0.05) were significant higher in diseased sites of chronic periodontitis patients than in healthy sites of healthy subjects. In addition, a significant correlation was found between the number of bacteria and various probing depth (P<0.001). There was no significantly difference between the proportion of T. forsythensis in subgingival plaque and probing depth.


CONCLUSIONS
Number of T. forsythensis are closely associated with periodontal status, and demonstrate the broad potential of real-time polymerase chain reaction application on periodontology.
Yang
He
H
Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu, China.
Meng
Shu
S
Zhao
Lei
L
Xu
Yi
Y
Wu
Ya-fei
YF
Zhao
Huan
H
D000328:Adult; D001439:Bacteroides; D055113:Chronic Periodontitis; D003773:Dental Plaque; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis