title: Mapping quantitative trait loci affecting susceptibility to Marek's disease virus in a backcross population of layer chickens.
abstract: Marek's disease (MD), caused by the oncogenic MD avian herpes virus (MDV), is a major source of economic losses to the poultry industry. A reciprocal backcross (BC) population (total 2052 individuals) was generated by crossing two partially inbred commercial Leghorn layer lines known to differ in MDV resistance, measured as survival time after challenge with a (vv+) MDV. QTL affecting resistance were identified by selective DNA pooling using a panel of 198 microsatellite markers covering two-thirds of the chicken genome. Data for each BC were analyzed separately, and as a combined data set. Markers showing significant association with resistance generally appeared in blocks of two or three, separated by blocks of nonsignificant markers. Defined this way, 15 chromosomal regions (QTLR) affecting MDV resistance, distributed among 10 chromosomes (GGA 1, 2, 3, 4, 5, 7, 8, 9, 15, and Z), were identified. The identified QTLR include one gene and three QTL associated with resistance in previous studies of other lines, and three additional QTL associated with resistance in previous studies of the present lines. These QTL could be used in marker-assisted selection (MAS) programs for MDV resistance and as a platform for high-resolution mapping and positional cloning of the resistance genes.
authors: Department of Animal Science, Iowa State University, Ames 50011, USA. eliyahu100@gmail.com
authors: E M
authors: EM
authors: Heifetz
authors: J E
authors: JE
authors: Fulton
authors: N P
authors: NP
authors: O'Sullivan
authors: J A
authors: JA
authors: Arthur
authors: J
authors: J
authors: Wang
authors: J C M
authors: JC
authors: Dekkers
authors: M
authors: M
authors: Soller
mesh_terms: D000818:Animals; D002645:Chickens; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D016678:Genome; D007113:Immunity, Innate / Q000235:genetics; D008380:Marek Disease / Q000235:genetics*; D011201:Poultry Diseases; D040641:Quantitative Trait Loci
chemical_list: D005819:Genetic Markers