Proteomic investigation of the aggregation phenomenon in Lactobacillus crispatus.
Aggregation process affects the ability of Lactobacillus crispatus, a probiotic, to survive into the gastro-intestinal environment and to adhere to the intestinal mucosa. To elucidate mechanisms underlying this process, a comparative proteomic study was carried out on a wild type strain M247 and its spontaneous isogenic mutant Mu5, which had lost the aggregative phenotype. Results highlighted an overall lower amount of enzymes involved in carbohydrate transport and metabolism in strain M247 compared to strain Mu5, suggesting a reduction in the general growth rate, probably caused by nutrient limitation in cell aggregates, coherently with the phenotypic traits of the strains. Moreover, the up-regulation of a putative elongation factor Tu in the wild type M247 strain could suggest a role of this particular protein in the adhesion mechanism of L. crispatus.
Siciliano
Rosa A
RA
Centro di Spettrometria di Massa Proteomica e Biomolecolare, Istituto di Scienze dell'Alimentazione del CNR, Avellino, Italy. rsiciliano@isa.cnr.it
Cacace
Giuseppina
G
Mazzeo
Maria F
MF
Morelli
Lorenzo
L
Elli
Marina
M
Rossi
Mauro
M
Malorni
Antonio
A
D001426:Bacterial Proteins / Q000145:classification / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D007778:Lactobacillus / Q000166:cytology / Q000378:metabolism*; D050296:Microbial Viability; D040901:Proteomics; D053719:Tandem Mass Spectrometry
D001426:Bacterial Proteins