[Early diagnosis of bladder cancer by special fluorescence spectrum: an in vitro experiment].
OBJECTIVE
To investigate the value of special fluorescence spectrum in the early diagnosis of bladder cancer.


METHODS
Hematoporphyrin monomethyl ether (HMME) of the concentrations of 10, 100, and 200 microg/ml, as photosensitizer, was added into the sample vessels of bladder cancer tissue to be incubated for 1 - 3 h. A detecting system consisting of solid state ultraviolet laser with the wavelength of 355 nm was used to measure the fluorescence spectrum.


RESULTS
The laser-induced auto-fluorescence (LIAF) of the bladder cancer tissue had characteristic double peaks at 445 - 490 nm. There was a remarkable characteristic peak, drug peak, located at 628.25 nm in the laser-induced drug-fluorescence (LIDF) of the bladder cancer tissue soaked by HMME.


CONCLUSIONS
Photosentisizer raise the sensitivity and specificity of fluorescence spectrum in diagnosis of bladder cancer. Stable, and with high strength and visible drug peak, the LIDF is may help improve the early diagnosis of bladder cancer.
Zou
Jun
J
Department of Urology, First People's Hospital of Guangzhou Affiliated to Guangzhou Medical College, Guangzhou 510180, China.
He
Hui-Chan
HC
Yao
Yong-Bang
YB
Dai
Qi-Shan
QS
Han
Zhao-Dong
ZD
Zhong
Wei-de
WD
D017323:Dihematoporphyrin Ether / Q000737:chemistry; D042241:Early Diagnosis; D006801:Humans; D066298:In Vitro Techniques; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D001749:Urinary Bladder Neoplasms / Q000737:chemistry / Q000175:diagnosis*
D017323:Dihematoporphyrin Ether