[The study in lasting effectivity on conditionally replicating adenovirus shRNA mediating survivin gene silencing in colon carcinoma cell line HT-29].
OBJECTIVE
To investigate the lasting effect of conditionally replicating adenovirus shRNA on survivin gene silencing in colon carcinoma cell lineHT-29.


METHODS
We transfected the colon cancer HT-29 cell with Ad-delE1b55KD-shRNA/Survivin-EGFP (control shRNA vector was the replication defective adenovirus called as Ad-shRNA/Survivin-EGFP). The expressions of EGFP, survivin mRNA and protein in HT-29 were detected at 1st day, 7th day, 14th day and 28th day after cell transfection.


RESULTS
The expression of EGFP in HT-29 was high at 7th day after cell transfection but survivin mRNA and protein expressions inhibited in each experiment group, among which Ad-delE1b55KD-shRNA/Survivin-EGFP group showed the most high EGFP expression; at 14th day, the expression of EGFP went down but the survivin mRNA and protein expressions upregulated obviously in replication defective adenovirus group and liposome vector group, however EGPF expression in HT-29 cell was still high and the survivin mRNA and protein expressions were still inhibited in Ad-delE1b55KD-shRNA/Survivin-EGFP group; at 28th day after cell HT-29 transfected, the expression of EGPF and the expression inhibition of survivin mRNA and protein disappeared in Ad-shRNA/Survivin-EGFP and liposome groups, but it was not in Ad-delE1b55KD-shRNA/Survivin-EGFP group (P < 0.05).


CONCLUSIONS
Conditionally replicating adenovirus shRNA can mediate the survivin gene silencing with long-lasting effects on colon carcinoma cell lines HT-29.
Wang
Chun-yi
CY
Department of General Surgery, the First Affiliated Hospital, Chongqing Medical University, Chongqing, China.
Fu
Zhong-xue
ZX
D000256:Adenoviridae / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D019073:HT29 Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D000077022:Survivin; D014162:Transfection
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D034741:RNA, Small Interfering; D000077022:Survivin; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins