title: [PDCD5 induces the apoptosis of human prostate cancer cells PC-3M-1E8].
abstract: OBJECTIVE
  To investigate the apoptosis-promoting effect of PDCD5 on human prostate cancer cells PC-3M-1E8.
  
  
  METHODS
  PCI-neo and PCI-neo-PDCD5 were transfected into PC-3M-1E8 cells by Lipofectamine 2000, the viability of the cells was analyzed by MTT assay 16 hours after removal of the serum, and the apoptosis was determined by in situ end-labeling and electron microscopy.
  
  
  RESULTS
  The viability and growing speed of the transfected cells were significantly decreased and their apoptotic indexes significantly increased as compared with the control group (P < 0.001).
  
  
  CONCLUSIONS
  PDCD5 may significantly inhibit the in vitro growth and promote the apoptosis of human prostate cancer cells PC-3M-1E8.
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongiiang 150081, China.
authors: Shu-jun
authors: SJ
authors: Li
authors: Jing
authors: J
authors: Yu
authors: Xue-fei
authors: XF
authors: Zhao
authors: Ying
authors: Y
authors: Jiang
authors: Zi-jun
authors: ZJ
authors: Liu
authors: Xiao-guang
authors: XG
authors: Yu
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000502:physiology*; D045744:Cell Line, Tumor; D006801:Humans; D020287:In Situ Nick-End Labeling; D008055:Lipids / Q000737:chemistry; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics / Q000502:physiology*; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection / Q000379:methods
chemical_list: D051017:Apoptosis Regulatory Proteins; D008055:Lipids; C086724:Lipofectamine; D009363:Neoplasm Proteins; C117283:PDCD5 protein, human