Evaluation of 5'-MGB hybridization probes for detection of Bordetella pertussis and Bordetella parapertussis using different real-time PCR instruments.
Rapid and accurate tests capable of distinguishing Bordetella pertussis from milder Bordetella parapertussis infection can aid in treating patients. We evaluated a novel real-time polymerase chain reaction (PCR) method that allows rapid and accurate diagnosis and distinction between B. pertussis and B. parapertussis infections. The method is based on a fluorescent 5'-minor groove-binding molecule hybridization probe that can clearly distinguish between B. pertussis and B. parapertussis by post-PCR melt curve analysis. The reagents worked equally well in several different real-time PCR instruments. The Bordetella detection reagents are combined with internal control components to account for PCR inhibition without compromising assay sensitivity.
Afonina
Irina
I
Nanogen Inc., Bothell, WA 98021, USA. iafonina@nanogen.com
Metcalf
Mark
M
Mills
Alan
A
Mahoney
Walt
W
D001885:Bordetella Infections / Q000175:diagnosis; D042483:Bordetella parapertussis / Q000235:genetics / Q000302:isolation & purification*; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D003937:Diagnosis, Differential; D006801:Humans; D015345:Oligonucleotide Probes / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D044366:Transition Temperature; D014917:Whooping Cough / Q000175:diagnosis
D004269:DNA, Bacterial; D015345:Oligonucleotide Probes