title: Lipopolysaccharide induces adipose differentiation-related protein expression and lipid accumulation in the liver through inhibition of fatty acid oxidation in mice.
abstract: BACKGROUND
  In the present study, we examined the effect of lipopolysaccharide (LPS) on liver histopathology with special reference to lipid metabolism in mice.
  
  
  METHODS
  Mice were injected with LPS intraperitoneally, and its effect on the liver was investigated pathologically and biochemically.
  
  
  RESULTS
  Oil-red O staining and adipose differentiation-related protein (ADRP) immunohistochemistry demonstrated that injection of LPS transiently induced lipid accumulation and ADRP expression in hepatocytes, especially around the portal vein. Microscopic observation revealed that lipid accumulation started 12 h after LPS injection. Time-course studies showed that LPS rapidly, within 2 h, decreased hepatic expression of nuclear hormone receptors, including peroxisome proliferator-activated receptor (PPAR) alpha. LPS inhibited the expression of PPARalpha-target genes involved in fatty acid oxidation in the liver such as those coding for enoyl-CoA hydratase, acyl-CoA dehydrogenase, and carnitine palmitoyl transferase-1, whereas LPS also suppressed the expression of genes related to fatty acid synthesis such as those for fatty acid synthase, stearoyl-CoA desaturase, and acetyl-CoA carboxylase alpha.
  
  
  CONCLUSIONS
  LPS induces transient lipid accumulation and expression of ADRP in the liver through inhibition of fatty acid oxidation by downregulation of the PPARalpha-related transcriptional mechanism.
authors: Department of General Medicine, Asahikawa Medical College, 2-1 Midorigaoka-higashi, Asahikawa 078-8510, Japan.
authors: Masumi
authors: M
authors: Ohhira
authors: Wataru
authors: W
authors: Motomura
authors: Mitsuko
authors: M
authors: Fukuda
authors: Takayuki
authors: T
authors: Yoshizaki
authors: Nobuhiko
authors: N
authors: Takahashi
authors: Satoshi
authors: S
authors: Tanno
authors: Nobutaka
authors: N
authors: Wakamiya
authors: Yutaka
authors: Y
authors: Kohgo
authors: Shima
authors: S
authors: Kumei
authors: Toshikatsu
authors: T
authors: Okumura
mesh_terms: D000818:Animals; D050356:Lipid Metabolism; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000473:pathology*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D010084:Oxidation-Reduction; D047493:PPAR alpha / Q000096:biosynthesis; D000070780:Perilipin-2; D015854:Up-Regulation
chemical_list: D008070:Lipopolysaccharides; D008565:Membrane Proteins; D047493:PPAR alpha; D000070780:Perilipin-2; C000602983:Plin2 protein, mouse