title: Loss of Hugl-1 expression associates with lymph node metastasis in endometrial cancer.
abstract: Mutation of neoplastic tumor suppressor genes, scribble, discs large, and lethal giant larvae (lgl), causes disruption of cell polarity and overproliferation of Drosophila epithelial cells and neuroblasts. Reduced expression of human homologue of lgl, Hugl-1, has been reported to be involved in development and progression of human colon cancer and malignant melanoma. To explore the association between Hugl-1 expression and clinical character in endometrial cancer, we examined the expression of Hugl-1 in primary endometrial cancer tissues. The expression of Hugl-1 mRNA in 86 primary endometrial cancer tissues was examined using semiquantitative reverse transcription polymerase chain reaction (RT-PCR). All samples were categorized into two groups: Hugl-1 positive and Hugl-1 negative. Clinical data of each group were analyzed by Fisher's exact probability test and survival rates of each group were compared by Kaplan-Meier method and Log-rank test. Loss of Hugl-1 expression had correlation with the higher incidence of lymph node metastasis, but not to the patient's age at onset, distant metastasis, clinical stage, lymph or venous vessel invasion, or histopathological grade of differentiation. The Hugl-1-positive group had poorer prognosis compared with the Hugl-1-negative group. These results indicate that loss of Hugl-1 expression in endometrial cancer may contribute to lymph node metastasis and it can be a factor of poor prognosis.
authors: Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-8655, Japan.
authors: Tetsushi
authors: T
authors: Tsuruga
authors: Shunsuke
authors: S
authors: Nakagawa
authors: Michiko
authors: M
authors: Watanabe
authors: Shin
authors: S
authors: Takizawa
authors: Yoko
authors: Y
authors: Matsumoto
authors: Kazunori
authors: K
authors: Nagasaka
authors: Kenbun
authors: K
authors: Sone
authors: Haruko
authors: H
authors: Hiraike
authors: Yuichiro
authors: Y
authors: Miyamoto
authors: Osamu
authors: O
authors: Hiraike
authors: Takeo
authors: T
authors: Minaguchi
authors: Katsutoshi
authors: K
authors: Oda
authors: Toshiharu
authors: T
authors: Yasugi
authors: Tetsu
authors: T
authors: Yano
authors: Yuji
authors: Y
authors: Taketani
mesh_terms: D003598:Cytoskeletal Proteins / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D011379:Prognosis
chemical_list: D003598:Cytoskeletal Proteins; C097906:LLGL1 protein, human