title: Elevated serum levels of human matrix metalloproteinase-9 (MMP-9) during the induction of peripheral blood stem cell mobilization by granulocyte colony-stimulating factor (G-CSF).
abstract: To investigate the role of matrix metalloproteinases (MMPs) in the mobilization of peripheral blood stem cells stimulated by granulocyte colony-stimulating factor (G-CSF), we analyzed MMP serum levels in 11 healthy donors and 9 patients who had hematological malignancies or germ cell tumors. A dose of 5-10 microg/kg per day of G-CSF (lenograstim) was administered for 4-8 days to each subject. The serum levels of MMP-2, and MMP-9; interleukin-3, -6, -8, and -10; stem cell factor; interferon-gamma; and tumor necrosis factor-alpha were measured both before and during G-CSF administration. MMP-9 was found to be increased in both the cancer patients and the healthy donor group. In contrast, the levels of each of the other factors tested were unchanged. No significant positive correlation was observed between the MMP-9 levels and the number of CD34+ cells. Hence, we found no significant role for MMPs during the mobilization of peripheral blood stem cells stimulated by G-CSF.
authors: Division of Oncology and Hematology, Department of Internal Medicine, Jikei University School of Medicine, 19-18 Nishishinbashi 3-chome, Minato-ku, Tokyo 105-8461, Japan. takexsaito@aol.com
authors: Takeshi
authors: T
authors: Saito
authors: Noriko
authors: N
authors: Usui
authors: Osamu
authors: O
authors: Asai
authors: Nobuaki
authors: N
authors: Dobashi
authors: Shingo
authors: S
authors: Yano
authors: Hiroshi
authors: H
authors: Osawa
authors: Yutaka
authors: Y
authors: Takei
authors: Shinobu
authors: S
authors: Takahara
authors: Yoji
authors: Y
authors: Ogasawara
authors: Hiroko
authors: H
authors: Otsubo
authors: Yuko
authors: Y
authors: Yamaguchi
authors: Jiro
authors: J
authors: Minami
authors: Yasutaka
authors: Y
authors: Hoshi
authors: Motoyuki
authors: M
authors: Kataoka
authors: Keisuke
authors: K
authors: Aiba
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D007378:Interleukins / Q000097:blood; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D008875:Middle Aged; D019089:Stem Cell Factor / Q000097:blood / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D018952:Antigens, CD34; D007378:Interleukins; D019089:Stem Cell Factor; D014409:Tumor Necrosis Factor-alpha; D016179:Granulocyte Colony-Stimulating Factor; D007371:Interferon-gamma; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9