title: Altered emotionality, spatial memory and cholinergic function in caveolin-1 knock-out mice.
abstract: Neurological phenotypes associated with loss of caveolin 1 (cav-1) (the defining structural protein in caveolar vesicles, which regulate signal transduction and cholesterol trafficking in cells) in mice have been reported recently. In brain, cav-1 is highly expressed in neurons and glia. We investigated emotional and cognitive behavioural domains in mice deficient in cav-1 (CavKO mice). CavKO mice were more anxious and spent more time in self-directed grooming behaviour than wild-type (wt) mice. In a spatial/working memory task, CavKO mice failed to recognize the object displacement, thus showing a spatial memory impairment. CavKO mice showed higher locomotor activity than wt mice, thus suggesting reduced inhibitory function by CNS cholinergic systems. Behavioural response to the cholinergic muscarinic antagonist, scopolamine (2 mg/Kg), was decreased in CavKO mice. Few behavioural sex differences emerged in mice; whereas the sex differences were generally attenuated or even reverted in the null genotype. Our data confirm a distinct behavioural phenotype in CavKO mice and indicate a selective alteration in central cholinergic function.
authors: Department Cell Biology and Neuroscience, Sect of Behavioural Neuroscience, Istituto Superiore di Sanità, Viale Regina Elena 299, Roma, Italy.
authors: Laura
authors: L
authors: Gioiosa
authors: Carla
authors: C
authors: Raggi
authors: Laura
authors: L
authors: Ricceri
authors: Jean-François
authors: JF
authors: Jasmin
authors: Philippe G
authors: PG
authors: Frank
authors: Franco
authors: F
authors: Capozza
authors: Michael P
authors: MP
authors: Lisanti
authors: Enrico
authors: E
authors: Alleva
authors: Massimo
authors: M
authors: Sargiacomo
authors: Giovanni
authors: G
authors: Laviola
mesh_terms: D000109:Acetylcholine / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects / Q000235:genetics; D051242:Caveolin 1 / Q000172:deficiency*; D002540:Cerebral Cortex / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004644:Emotions / Q000502:physiology*; D005106:Exploratory Behavior / Q000187:drug effects; D005260:Female; D008297:Male; D008569:Memory Disorders / Q000235:genetics*; D008570:Memory, Short-Term / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018727:Muscarinic Antagonists / Q000494:pharmacology; D012601:Scopolamine / Q000494:pharmacology; D012737:Sex Factors
chemical_list: D051242:Caveolin 1; D018727:Muscarinic Antagonists; D012601:Scopolamine; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine