Maximal exercise test is a useful method for physical capacity and oxygen consumption determination in streptozotocin-diabetic rats.
BACKGROUND
The aim of the present study was to investigate the relationship between speed during maximum exercise test (ET) and oxygen consumption (VO2) in control and STZ-diabetic rats, in order to provide a useful method to determine exercise capacity and prescription in researches involving STZ-diabetic rats.


METHODS
Male Wistar rats were divided into two groups: control (CG, n = 10) and diabetic (DG, n = 8). The animals were submitted to ET on treadmill with simultaneous gas analysis through open respirometry system. ET and VO2 were assessed 60 days after diabetes induction (STZ, 50 mg/Kg).


RESULTS
VO2 maximum was reduced in STZ-diabetic rats (72.5 +/- 1 mL/Kg/min-1) compared to CG rats (81.1 +/- 1 mL/Kg/min-1). There were positive correlations between ET speed and VO2 (r = 0.87 for CG and r = 0.8 for DG), as well as between ET speed and VO2 reserve (r = 0.77 for CG and r = 0.7 for DG). Positive correlations were also obtained between measured VO2 and VO2 predicted values (r = 0.81 for CG and r = 0.75 for DG) by linear regression equations to CG (VO2 = 1.54 * ET speed + 52.34) and DG (VO2 = 1.16 * ET speed + 51.99). Moreover, we observed that 60% of ET speed corresponded to 72 and 75% of VO2 reserve for CG and DG, respectively. The maximum ET speed was also correlated with VO2 maximum for both groups (CG: r = 0.7 and DG: r = 0.7).


CONCLUSIONS
These results suggest that: a) VO2 and VO2 reserve can be estimated using linear regression equations obtained from correlations with ET speed for each studied group; b) exercise training can be prescribed based on ET in control and diabetic-STZ rats; c) physical capacity can be determined by ET. Therefore, ET, which involves a relatively simple methodology and low cost, can be used as an indicator of cardio-respiratory capacity in future studies that investigate the physiological effect of acute or chronic exercise in control and STZ-diabetic male rats.
Rodrigues
Bruno
B
Hypertension Unit, Heart Institute (InCor), Medical School, University of Sao Paulo, Sao Paulo-Sao Paulo, Brazil. bruno.rodrigues@incor.usp.br
Figueroa
Diego M
DM
Mostarda
Cristiano T
CT
Heeren
Marcelo V
MV
Irigoyen
Maria-Cláudia
MC
De Angelis
Kátia
K
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D008297:Male; D010101:Oxygen Consumption; D010805:Physical Conditioning, Animal; D010809:Physical Fitness; D051381:Rats; D017208:Rats, Wistar; D012044:Regression Analysis; D015203:Reproducibility of Results