Multiplex PCR that can distinguish between immunologically cross- reactive serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains.
We describe a highly sensitive and specific multiplex PCR, based on capsular loci and the species specific apxIV gene, that unequivocally differentiates serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains that are cross-reactive in conventional immunological tests.
Zhou
L
L
Department of Paediatrics, Imperial College London, St. Mary's Campus, London W2 1PG, United Kingdom.
Jones
S C P
SC
Angen
Ø
Ø
Bossé
J T
JT
Nash
J H E
JH
Frey
J
J
Zhou
R
R
Chen
H C
HC
Kroll
J S
JS
Rycroft
A N
AN
Langford
P R
PR
D000189:Actinobacillus Infections / Q000382:microbiology*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D016667:Bacterial Capsules / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D003429:Cross Reactions / Q000276:immunology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
C122177:ApxIVA protein, Actinobacillus pleuropneumoniae; D001426:Bacterial Proteins; D004269:DNA, Bacterial