title: Primary and secondary infections of Macaca fascicularis monkeys with Asian and American genotypes of dengue virus 2.
abstract: The goal of this study was to compare the immune response and the protection capacity induced by the dengue virus 2 (DENV-2) American and Asian genotypes in Macaca fascicularis monkeys. Animals were infected with American or Asian DENV-2 strains and challenged 1 year later with a DENV-2 Asian genotype strain. The viremia and monkey antibody levels were similar for the different strains after primary and secondary infection; however, the functionality of the antibody response was different. A limited viral replication was demonstrated after the secondary infection in all the monkeys. No virus was isolated in tissue culture, while reverse transcription-PCR showed a late positive reaction in four of five challenged monkeys. The immunoglobulin M response pattern and the detection of antibodies to specific proteins by Western blotting supported the protection data. Despite the demonstration of the protective effect after homologous challenge, a strong anamnestic antibody response was observed.
authors: Department of Virology, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía, km 6 1/2, P.O. Box 601, Marianao 13, Havana, Cuba.
authors: Lidice
authors: L
authors: Bernardo
authors: Alienys
authors: A
authors: Izquierdo
authors: Irina
authors: I
authors: Prado
authors: Delfina
authors: D
authors: Rosario
authors: Mayling
authors: M
authors: Alvarez
authors: Emidalys
authors: E
authors: Santana
authors: Jorge
authors: J
authors: Castro
authors: Rafael
authors: R
authors: Martínez
authors: Rosmari
authors: R
authors: Rodríguez
authors: Luis
authors: L
authors: Morier
authors: Gerardo
authors: G
authors: Guillén
authors: María G
authors: MG
authors: Guzmán
mesh_terms: D000330:Aedes; D000569:Americas; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001208:Asia; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003715:Dengue / Q000276:immunology* / Q000503:physiopathology / Q000821:virology; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D005838:Genotype; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D008252:Macaca fascicularis; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019595:Severe Dengue / Q000276:immunology* / Q000503:physiopathology / Q000821:virology; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M