Superoxide dismutase-1 influences the timing and post-hypoxic stability of neonatal breathing.
Reactive oxygen species (ROS) likely play a role in the hypoxic ventilatory response. We determined whether hypoxic responses were influenced by alterations in cellular redox status induced by reductions in superoxide dismutase-1 (SOD-1) activity, a cytosolic anti-oxidant enzyme. Using whole-body, continuous-flow plethysmography, we compared ventilatory responses to moderate hypoxia (10% inspired O2) of Sod1+/+, +/- and -/- postnatal day 4 (P4) littermates. Sod1+/- neonates exhibited a consistently lower breathing frequency than their wild-type littermates, regardless of inspired O2 level. While SOD-1 deficiency had no effect on the magnitude of the ventilatory response during hypoxia, it did compromise stability of breathing in the post-hypoxic period. Our results suggest SOD-1 stimulates ventilation and helps stabilize breathing after a hypoxic perturbation.
Cummings
Kevin J
KJ
Department of Physiology and Biophysics, Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada T2N 4N1.
Kalf
Dan
D
Moore
Sherry
S
Miller
B Joan
BJ
Jirik
Frank R
FR
Wilson
Richard J A
RJ
D000818:Animals; D000831:Animals, Newborn; D005838:Genotype; D000860:Hypoxia / Q000503:physiopathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012119:Respiration / Q000235:genetics*; D013482:Superoxide Dismutase / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D000072105:Superoxide Dismutase-1
C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1