Diversity of the active methanotrophic community in acidic peatlands as assessed by mRNA and SIP-PLFA analyses.
The active methanotroph community was investigated for the first time in heather (Calluna)-covered moorlands and Sphagnum/Eriophorum-covered UK peatlands. Direct extraction of mRNA from these soils facilitated detection of expression of methane monooxygenase genes, which revealed that particulate methane monooxygenase and not soluble methane monooxygenase was probably responsible for CH(4) oxidation in situ, because only pmoA transcripts (encoding a subunit of particulate methane monooxygenase) were readily detectable. Differences in methanotroph community structures were observed between the Calluna-covered moorland and Sphagnum/Eriophorum-covered gully habitats. As with many other Sphagnum-covered peatlands, the Sphagnum/Eriophorum-covered gullies were dominated by Methylocystis. Methylocella and Methylocapsa-related species were also present. Methylobacter-related species were found as demonstrated by the use of a pmoA-based diagnostic microarray. In Calluna-covered moorlands, in addition to Methylocella and Methylocystis, a unique group of peat-associated type I methanotrophs (Gammaproteobacteria) and a group of uncultivated type II methanotrophs (Alphaproteobacteria) were also found. The pmoA sequences of the latter were only distantly related to Methylocapsa and also to the RA-14 group of methanotrophs, which are believed to be involved in oxidation of atmospheric concentrations of CH(4). Soil samples were also labelled with (13)CH(4), and subsequent analysis of the (13)C-labelled phospholipid fatty acids (PLFAs) showed that 16:1 omega 7, 18:1 omega 7 and 18:1 omega 9 were the major labelled PLFAs. The presence of (13)C-labelled 18:1 omega 9, which was not a major PLFA of any extant methanotrophs, indicated the presence of novel methanotrophs in this peatland.
Chen
Yin
Y
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
Dumont
Marc G
MG
McNamara
Niall P
NP
Chamberlain
Paul M
PM
Bodrossy
Levente
L
Stralis-Pavese
Nancy
N
Murrell
J Colin
JC
D020561:Alphaproteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D029795:Calluna / Q000254:growth & development; D002247:Carbon Isotopes / Q000378:metabolism; D017753:Ecosystem; D005227:Fatty Acids / Q000032:analysis; D014644:Genetic Variation; D006863:Hydrogen-Ion Concentration; D008697:Methane / Q000378:metabolism*; D008772:Methylococcaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D042083:Methylocystaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D020411:Oligonucleotide Array Sequence Analysis; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D020560:Proteobacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology; D044003:Sphagnopsida / Q000254:growth & development; D006113:United Kingdom
D002247:Carbon Isotopes; D005227:Fatty Acids; D010743:Phospholipids; D012333:RNA, Messenger; D012987:Soil; D010105:Oxygenases; C021555:methane monooxygenase; D008697:Methane