title: Relative influence of the adeno-associated virus (AAV) type 2 p5 element for recombinant AAV vector site-specific integration.
abstract: The p5 promoter region of the adeno-associated virus type 2 (AAV-2) rep gene has been described as essential for Rep-mediated site-specific integration (RMSSI) of plasmid sequences in human chromosome 19. We report here that insertion of a full-length or minimal p5 element between the viral inverted terminal repeats does not significantly increase RMSSI of a recombinant AAV (rAAV) vector after infection of growth-arrested or proliferating human cells. This result suggests that the p5 element may not improve RMSSI of rAAV vectors in vivo.
authors: INSERM U758, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, F-69007 Lyon, France.
authors: Mickaël
authors: M
authors: Guilbaud
authors: Gilliane
authors: G
authors: Chadeuf
authors: Fabio
authors: F
authors: Avolio
authors: Achille
authors: A
authors: François
authors: Philippe
authors: P
authors: Moullier
authors: Alessandra
authors: A
authors: Recchia
authors: Anna
authors: A
authors: Salvetti
mesh_terms: D001483:Base Sequence; D002888:Chromosomes, Human, Pair 19; D017931:DNA Primers; D000229:Dependovirus / Q000235:genetics*; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D010957:Plasmids; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D016662:Virus Integration
chemical_list: D017931:DNA Primers