title: CD437 induces apoptosis in ovarian adenocarcinoma cells via ER stress signaling.
abstract: A synthetic retinoid, CD437, has been shown to exert potent anti-tumor activity against various types of cancer cell lines, regardless of their sensitivities to natural retinoids. We herein demonstrate that CD437 induces endoplasmic reticulum (ER) stress, including the up-regulation of CHOP, BIP and GADD34 mRNA through ER stress transducer (PERK and IRE1alpha) activation in an ovarian adenocarcinoma cell line, SKOV3. It was also shown that CD437 induced the CHOP and GADD34 expressions in another four ovarian adenocarcinoma cell lines, indicating that CD437 functions as an ER stress inducer in these cell lines. Moreover, the siRNA-mediated knockdown of inducible CHOP expression prevented the cytotoxic effect of CD437. These results suggest that ER stress plays an important role in the mechanism by which CD437 induces apoptosis in ovarian adenocarcinoma cells.
authors: Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, Nishi-cho 86, Yonago 683-8504, Japan.
authors: Yumi
authors: Y
authors: Watanabe
authors: Hiroyuki
authors: H
authors: Tsuchiya
authors: Tomohiko
authors: T
authors: Sakabe
authors: Saori
authors: S
authors: Matsuoka
authors: Yuji
authors: Y
authors: Akechi
authors: Yuka
authors: Y
authors: Fujimoto
authors: Keitaro
authors: K
authors: Yamane
authors: Remina
authors: R
authors: Ikeda
authors: Ren
authors: R
authors: Nishio
authors: Kei
authors: K
authors: Terabayashi
authors: Kyoko
authors: K
authors: Ishii
authors: Kazue
authors: K
authors: Gonda
authors: Yoshiaki
authors: Y
authors: Matsumi
authors: An Afida
authors: AA
authors: Ashla
authors: Hideharu
authors: H
authors: Okamoto
authors: Kazuko
authors: K
authors: Takubo
authors: Akiko
authors: A
authors: Tomita
authors: Yoshiko
authors: Y
authors: Hoshikawa
authors: Akihiro
authors: A
authors: Kurimasa
authors: Hiroaki
authors: H
authors: Itamochi
authors: Tasuku
authors: T
authors: Harada
authors: Naoki
authors: N
authors: Terakawa
authors: Goshi
authors: G
authors: Shiota
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D012176:Retinoids / Q000008:administration & dosage*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: C099555:CD 437; D012176:Retinoids