Effect of mesalamine on healing in experimental colon anastomosis: a randomised experimental study.
OBJECTIVE
We aimed to investigate the effect of mesalamine on healing of experimental colon anastomosis model.


METHODS
Forty adult male Wistar albino rats were performed segmentary colonic resection and end-to-end anastomosis. Animals were randomly divided into four groups: group I, anastomosis group, received no treatment (GI, n=8); group II, anastomosis+oral mesalamine group (100mg/kg/day); group III, anastomosis+rectal mesalamine (2mL) group, (GIII, n=8); group IV, anastomosis+oral mesalamine+rectal mesalamine (GIV, n=8) group. A sham group (n=8) was constituted and was performed laparotomy. Bursting pressure, hydroxyproline levels and histopathological characteristics of the anastomosis were analyzed.


RESULTS
Although it was not statistically significant, there was an increase in the burst pressure of the mesalamine group. When hydroxyproline measurements were compared there were statistically significant difference between the non-treated colon and all groups. There were significant differences between GI and GIII-GIV, GII and GIV. The differences between group I and II and group II and III were not statistically significant. When we compared the median amount of the histopathological changes, we found significant difference between the anastomosis and the mesalamine groups (P<0.05). But when mesalamine groups were compared with each other we did not observe a significant difference.


CONCLUSIONS
Mesalamine had positive effects which were not statistically significant on bursting pressure and statistically different significant effects on hydroxyproline (HP) levels based on the way of administration and statistically significant positive effects on histopathologic anastomotic healing in experimental anastomosis model.
Aslan
Ahmet
A
Mustafa Kemal University, Faculty of Medicine, Department of General Surgery, Antakya-Hatay, Turkey. aslan@mku.edu.tr
Temiz
Muhyittin
M
Hakverdi
Sibel
S
Polat
Gurbuz
G
Tumer
Cemil
C
Temiz
Abdulkerim
A
Canbolant
Elif
E
D000714:Anastomosis, Surgical; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D003106:Colon / Q000601:surgery*; D006909:Hydroxyproline / Q000032:analysis; D008297:Male; D019804:Mesalamine / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013536:Suture Techniques; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D019804:Mesalamine; D006909:Hydroxyproline