Dual-reporter phenotypic assay for human immunodeficiency viruses.
We have established a novel human immunodeficiency virus (HIV) tandem-reporter assay using HIV receptor-transduced NP-2 cells with long terminal repeat-controlled beta-galactosidase, inserted internal ribosome entry site, and secretary alkaline phosphatase genes. This assay allows users to detect replication of clinical isolates, indicating its useful application as an HIV phenotypic assay.
Kajiwara
Keiko
K
Laboratory of Virus Immunology, Institute for Virus Research, Kyoto University, 53 Kawaramachi, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Kodama
Eiichi
E
Sakagami
Yasuko
Y
Naito
Takeshi
T
Matsuoka
Masao
M
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D019380:Anti-HIV Agents / Q000494:pharmacology; D002460:Cell Line; D017930:Genes, Reporter; D006678:HIV / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D015658:HIV Infections / Q000175:diagnosis*; D016325:HIV Long Terminal Repeat / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D014773:Virology / Q000379:methods*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D019380:Anti-HIV Agents; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase