[Genome cloning and phylogenetic analysis of human bocavirus capsid gene].
The full-length genome of one human bocavirus (HBoV) and the VP1 sequences of nine HBoV were amplified from patients' samples by PCR, cloned into pGEM-T vector separately, and sequenced. In this study, the one full length gemome and nine VP1 sequences of HBoV were aligened with 14 sequences of Parvoviruses which were canonical exemplars in Parvovirinae. Phylogenetic analysis showed that HBoV capsid sequences positioned closely to B19 parvovirus, although they positioned far in phylogenetic tree based on full length genome. Many similarities were found between HBoV and B19 in capsid by alignment on secondary structural elements. Because both B19 and HBoV are the only Parvoviruses that infect mankind, so study on HBoV may be used for reference to B19 which had been studied for about 30 years. By analysis of mutational sites, HBoV capsid protein showed a highly conserved secondary structural elements, but highly active in VP1-U, leading end of VP2 and insertions between the strands of the betaG-H. This cued that HBoV inclined to immune evasion and infectant adaptive faculty.
Qi
Zheng-Yu
ZY
Institute for Biochemistry and Nutrition, Guizhou University, Guiyang 550025, China.
Qu
Xiao-Wang
XW
Liu
Wen-Pei
WP
Xie
Zhi-Ping
ZP
Gao
Han-Chun
HC
Zheng
Li-Shu
LS
Kuang
Zhi-Zhou
ZZ
Yu
Jian-Ping
JP
Duan
Zhao-Jun
ZJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D052660:Bocavirus / Q000145:classification / Q000235:genetics*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction
D036022:Capsid Proteins