Crystallization and preliminary crystallographic analysis of the Tob-hCaf1 complex.
The Tob/BTG family is a group of antiproliferative proteins that contain two highly homologous regions named Box A and Box B. These proteins all associate with CCR4-associated factor 1 (Caf1), which belongs to the ribonuclease D family of deadenylases. The antiproliferative region of human Tob (residues 1-138) and intact hCaf1 were co-expressed in Escherichia coli, purified and successfully cocrystallized. The crystal belongs to the tetragonal space group I422, with unit-cell parameters a = b = 150.9, c = 113.9 A, and is estimated to contain one heterodimer per asymmetric unit. The crystal diffracted to around 2.6 A resolution.
Nishida
Kinya
K
Department of Structural Biology, Graduate School of Pharmaceutical Science, Hokkaido University, N12, W6, Kita-ku, Sapporo 060-0812, Japan.
Horiuchi
Masataka
M
Noda
Nobuo N
NN
Takahasi
Kiyohiro
K
Iwasaki
Norimasa
N
Minami
Akio
A
Inagaki
Fuyuhiko
F
D003460:Crystallization; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014961:X-Ray Diffraction
C489780:CNOT8 protein, human; D047908:Intracellular Signaling Peptides and Proteins; C098669:TOB1 protein, human; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins