Assimilation of homotaurine-nitrogen by Burkholderia sp. and excretion of sulfopropanoate.
Homotaurine (3-aminopropanesulfonate), free or derivatized, is in widespread pharmaceutical and laboratory use. Studies with enrichment cultures indicated that the compound is degradable as a sole source of carbon or as a sole source of nitrogen for bacterial growth. A pure culture of Burkholderia sp. was isolated which assimilated the amino group from homotaurine in a glucose-salts medium, and which released an organosulfonate, 3-sulfopropanoate, into the medium stoichiometrically. The deamination involved an inducible 2-oxoglutarate-dependent aminotransferase to yield glutamate, and 3-sulfopropanal. Release of the amino group was attributed to the measured NADP-coupled glutamate dehydrogenase.
Mayer
Jutta
J
Department of Biology University of Konstanz, Konstanz, Germany.
Denger
Karin
K
Kaspar
Katrin
K
Hollemeyer
Klaus
K
Smits
Theo H M
TH
Huhn
Thomas
T
Cook
Alasdair M
AM
D019117:Burkholderia / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D018086:Glutamate Dehydrogenase (NADP+) / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism; D008954:Models, Biological; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism*; D011422:Propionates / Q000378:metabolism*; D013654:Taurine / Q000031:analogs & derivatives* / Q000378:metabolism; D000637:Transaminases / Q000378:metabolism
C527580:3-sulfopropanoate; D003470:Culture Media; D007656:Ketoglutaric Acids; D011422:Propionates; D013654:Taurine; D018698:Glutamic Acid; C001355:tramiprosate; D018086:Glutamate Dehydrogenase (NADP+); D000637:Transaminases; D009584:Nitrogen