title: Composition and distribution of carrabiose moieties in hybrid kappa-/iota-carrageenans using carrageenases.
abstract: Hybrid kappa/iota-carrageeans extracted from Gigartina skottsbergii, Chondracanthus chamissoï, and Chondrus crispus were incubated with Pseudoalteromonas carrageenovora kappa-carrageenase and Alteromonas fortis iota-carrageenase. The degradation products as well as the resistant fraction were fully characterized by chromatography, NMR, and mass spectrometry. The low percentage of degradation observed after treatment by the iota-carrageenase suggests that long segments of iota-carrabiose or block of iota-carrageenan are low in abundance. The degradation products of the kappa-carrageenase allow discriminating three modes of kappa-carrabiose distribution: blocks of kappa-carrabiose, kappa-carrabiose rich fraction containing iota-carrabiose units probably randomly distributed, and iota-carrabiose rich fraction which corresponds to the resistant fraction. The proportions of each fraction were related to the botanical origin as well as the place of growth of the seaweed.
authors: Université Pierre et Marie Curie, Paris VI-CNRS, Végétaux Marins et Biomolécules, UMR 7139, Station Biologique, F-29680 Roscoff, France.
authors: Marion
authors: M
authors: Guibet
authors: Patrick
authors: P
authors: Boulenguer
authors: Jacques
authors: J
authors: Mazoyer
authors: Nelly
authors: N
authors: Kervarec
authors: Aristotelis
authors: A
authors: Antonopoulos
authors: Michel
authors: M
authors: Lafosse
authors: William
authors: W
authors: Helbert
mesh_terms: D002351:Carrageenan / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004798:Enzymes / Q000737:chemistry*
chemical_list: D004798:Enzymes; C080061:carrabiose; D002351:Carrageenan