Selective fluorescence detection of 8-oxoguanosine with 8-oxoG-clamp.
8-oxoguanosine, which is derived from the oxidation of guanosine (dG), is known to induce transversion mutations (G:C-->T:A) in DNA. The compounds with a small molecular weight for recognizing 8-oxoG were designed on the basis of the structure of the G-clamp, which is reported to have selective affinity toward guanosine. The G-clamp derivatives with the additional binding units toward 8-oxoG were effectively synthesized and named "8-oxoG-clamps." The 8-oxoG-clamp completely discriminated 8-oxoG from other nucleosides by fluorescence quenching.
Nakagawa
Osamu
O
Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Ono
Sayaka
S
Tsujimoto
Akira
A
Li
Zhichun
Z
Sasaki
Shigeki
S
D002219:Carbamates / Q000032:analysis* / Q000737:chemistry; D005453:Fluorescence; D006151:Guanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011744:Pyrimidinones / Q000032:analysis* / Q000737:chemistry; D017186:Titrimetry
D002219:Carbamates; C522074:Cbz-8-oxoG-clamp; D011744:Pyrimidinones; D006151:Guanosine; C046215:8-hydroxyguanosine