title: Proteomic investigation of the aggregation phenomenon in Lactobacillus crispatus.
abstract: Aggregation process affects the ability of Lactobacillus crispatus, a probiotic, to survive into the gastro-intestinal environment and to adhere to the intestinal mucosa. To elucidate mechanisms underlying this process, a comparative proteomic study was carried out on a wild type strain M247 and its spontaneous isogenic mutant Mu5, which had lost the aggregative phenotype. Results highlighted an overall lower amount of enzymes involved in carbohydrate transport and metabolism in strain M247 compared to strain Mu5, suggesting a reduction in the general growth rate, probably caused by nutrient limitation in cell aggregates, coherently with the phenotypic traits of the strains. Moreover, the up-regulation of a putative elongation factor Tu in the wild type M247 strain could suggest a role of this particular protein in the adhesion mechanism of L. crispatus.
authors: Centro di Spettrometria di Massa Proteomica e Biomolecolare, Istituto di Scienze dell'Alimentazione del CNR, Avellino, Italy. rsiciliano@isa.cnr.it
authors: Rosa A
authors: RA
authors: Siciliano
authors: Giuseppina
authors: G
authors: Cacace
authors: Maria F
authors: MF
authors: Mazzeo
authors: Lorenzo
authors: L
authors: Morelli
authors: Marina
authors: M
authors: Elli
authors: Mauro
authors: M
authors: Rossi
authors: Antonio
authors: A
authors: Malorni
mesh_terms: D001426:Bacterial Proteins / Q000145:classification / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D007778:Lactobacillus / Q000166:cytology / Q000378:metabolism*; D050296:Microbial Viability; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins