Effect of the incorporation of 2'-deoxy-8-(hydroxyl)adenosine on the stability of quadruplexes formed by modified human telomeric DNA.
Differential scanning calorimetry (DSC) and circular dichroism (CD) techniques were used to investigate the physico-chemical properties of the quadruplexes formed by the two different truncations of human telomeric sequence d(TAGGGT) and d(AGGGT), where the adenines were substituted by 2'-deoxy-8-(hydroxyl)adenosine (A --> A OH). CD spectra show that the modified sequences are able to form parallel-stranded quadruplex structure. Analysis of the thermodynamic parameters reveals that the introduction of the modified adenine affects in different way the thermal stability of the [d(TAGGGT)]4 and [d(AGGGT)]4 quadruplexes.
Petraccone
Luigi
L
Dipartimento di Chimica, Università degli Studi di Napoli Federico II, Napoli, Italy.
Duro
Ida
I
Erra
Eva
E
Randazzo
Antonio
A
Virno
Ada
A
Giancola
Concetta
C
D000241:Adenosine / Q000378:metabolism*; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D003839:Deoxyadenosines / Q000378:metabolism*; D054856:G-Quadruplexes; D006801:Humans; D009691:Nucleic Acid Denaturation; D016615:Telomere / Q000378:metabolism*; D013816:Thermodynamics
C436623:8-hydroxy-2'-deoxyadenosine; D003839:Deoxyadenosines; D004247:DNA; D000241:Adenosine