Synthesis of the 3'-thio-nucleosides and subsequent automated synthesis of oligodeoxynucleotides containing a 3'-S-phosphorothiolate linkage.
Oligodeoxynucleotides containing 3'-S-phosphorothiolate (3'-PS) linkages have become useful tools for probing enzyme-catalyzed cleavage processes in DNA. This protocol describes the synthesis of the phosphorothioamidite monomers derived from thymidine and 2'-deoxycytidine, and their application to a fully automated procedure for synthesising oligodeoxynucleotides containing 3'-PS linkages. The synthesis of the 5'-protected-3'-amidites is achievable in 2 weeks with the DNA synthesis and purification taking another 1 week.
Gaynor
James W
JW
Department of Chemistry, University of Liverpool, Liverpool, L69 7ZD, UK. J.W.Gaynor@liverpool.ac.uk
Bentley
Joanne
J
Cosstick
Richard
R
D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis*; D013872:Thionucleosides / Q000138:chemical synthesis*
D009841:Oligonucleotides; D013872:Thionucleosides