Colorimetric detection of multidrug-resistant or extensively drug-resistant tuberculosis by use of malachite green indicator dye.
The malachite green microtube (MGMT) susceptibility assay was performed directly on sputum specimens (n = 80) and indirectly on Mycobacterium tuberculosis clinical isolates (n = 60). The technique is based on the malachite green dye, which changes color in response to M. tuberculosis growth. The MGMT assay is simple and rapid and does not require expensive instruments.
Farnia
Parissa
P
Mycobacteriology Research Centre, National Research Institute of Tuberculosis and Lung Disease, Shaheed Bahonar Ave., Darabad, Tehran 19556, PO Box 19575/154, Iran. pfarnia@hotmail.com
Masjedi
Mohammad Reza
MR
Mohammadi
Foroozan
F
Tabarsei
Payam
P
Farnia
Poopak
P
Mohammadzadeh
Ali Reza
AR
Baghei
Parvaneh
P
Varahram
Mohammad
M
Hoffner
Sven
S
Velayati
Ali Akbar
AA
D000995:Antitubercular Agents / Q000494:pharmacology; D003124:Colorimetry / Q000379:methods*; D054908:Extensively Drug-Resistant Tuberculosis / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D012394:Rosaniline Dyes / Q000378:metabolism; D013183:Sputum / Q000382:microbiology; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis* / Q000382:microbiology
D000995:Antitubercular Agents; D012394:Rosaniline Dyes; C005095:malachite green