Neuroendocrine secretory protein 55 (NESP55) in the spinal cord of rat: an immunocytochemical study.
The immunohistochemical expression of a novel chromogranin-like protein, neuroendocrine secretory protein 55 (NESP55), in the rat spinal cord was investigated. NESP55-immunoreactive cells were detected in the ventral horn, intermediate laminae, and deep dorsal horn, comprising motoneurons, autonomic neurons, and interneurons throughout all spinal segments. Within laminae I-II of the dorsal horn, one or two NESP55-positive cells were often seen. Nerve fibers also contained NESP55 immunoreactivity (IR) and were particularly prominent in the ventral horn. No nerve terminals/varicosities appeared to contain NESP55 in any spinal lamina. Double-staining experiments revealed that a high proportion of the NESP55-positive neurons were cholinergic. Moreover, NESP55-IR in the motoneurons was evenly distributed in the whole cytoplasm with a finely granular appearance. In contrast, the fluorescent material in the preganglionic neurons was concentrated in the perinuclear region and largely overlapped with the trans-Golgi network marker TGN38. Our data provide detailed morphological information on the distribution of NESP55-IR in the rat spinal cord. Also, the differential intracellular expression of NESP55-IR in the spinal motoneurons and autonomic neurons suggests that NESP55 may be processed into different secretory granules and may be involved in both constitutive and regulated pathways in these neurons.
Li
Yongling
Y
Department of Anatomy and Cell Biology, Institute of Biomedicine, Göteborg University, SE-405 30 Göteborg, Sweden. yongling.li@anatcell.gu.se
Fischer-Colbrie
Reiner
R
Dahlström
Annica
A
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D001341:Autonomic Nervous System / Q000166:cytology / Q000378:metabolism; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D002864:Chromogranins; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000378:metabolism*; D007150:Immunohistochemistry; D007395:Interneurons / Q000166:cytology / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009489:Neurosecretion / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D022142:Secretory Vesicles / Q000378:metabolism / Q000648:ultrastructure; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*
D002864:Chromogranins; D008562:Membrane Glycoproteins; C513045:Tgoln2 protein, rat; C498643:Gnas protein, rat; D019205:GTP-Binding Protein alpha Subunits, Gs; D000109:Acetylcholine