title: Exploratory analysis of Fas gene polymorphisms in pediatric osteosarcoma patients.
abstract: Defective apoptosis signaling by the Fas pathway has carcinogenic implications. We analyzed 123 pediatric patients with osteosarcoma for Fas single nucleotide polymorphisms: 2 of the promoter region (-1377 G>A and -670 A>G) and 2 of the coding region (exon 3 18272 A>G and exon 7 22628 C>T). As a comparison group, we used 510 adults without a history of cancer. We found an increased risk of osteosarcoma associated with the heterozygous genotype Fas exon 3 AG (genotype frequency 19.5% in cases vs. 12.0% in controls, P=0.028; adjusted odds ratio=1.6, 95% confidence interval=0.9-2.7], and this association was more pronounced in non-Hispanic whites (20.6% in cases vs. 10.1% in controls, P=0.014; adjusted odds ratio=2.3, 95% confidence interval=1.2-4.6). Additionally, the frequency of the variant allele (exon 3 G) was significantly higher in cases than in controls for both the entire group and non-Hispanic whites (P=0.046 and P=0.030, respectively). We found no significant association between osteosarcoma risk and the other Fas polymorphisms. This study suggests an association between the Fas exon 3 A>G polymorphism and osteosarcoma risk; however, further study is needed with pediatric controls and a larger sample size.
authors: Department of Pediatrics, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA.
authors: Nadezhda V
authors: NV
authors: Koshkina
authors: Eugenie S
authors: ES
authors: Kleinerman
authors: Guojun
authors: G
authors: Li
authors: Chong C
authors: CC
authors: Zhao
authors: Qingyi
authors: Q
authors: Wei
authors: Erich M
authors: EM
authors: Sturgis
mesh_terms: D000328:Adult; D001859:Bone Neoplasms / Q000235:genetics*; D002648:Child; D005091:Exons; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012016:Reference Values; D019014:fas Receptor / Q000235:genetics*
chemical_list: C491901:FAS protein, human; D019014:fas Receptor