Retinoic acid promotes mouse splenic B cell surface IgG expression and maturation stimulated by CD40 and IL-4.
Retinoic acid (RA) increases antibody production in vivo but its role in B-cell activation is unclear. In a model of purified mouse splenic B cells stimulated by CD40 coreceptor (as a surrogate of T cell co-stimulation), IL-4, a principal Th-2 cytokine, and ligation of the B-cell antigen receptor, CD40 engagement or IL-4 alone induced B-cell activation indicated by increased Ig gamma1 germline transcripts, cell proliferation, and surface (s)IgG1 expression, while triple stimulation with the combination of anti-CD40/IL-4/anti-mu synergized to heighten B-cell activation. Although RA was growth inhibitory for anti-CD40-activated B cells, RA increased the proportion of B cells that had more differentiated phenotypes, such as expression of higher level of activation-induced deaminase, Blimp-1, CD138/syndecan-1 and sIgG1. Overall, RA can promote B-cell maturation at the population level by increasing the number of sIgG1 and CD138 expressing cells, which may be related to the potentiation of humoral immunity in vivo.
Chen
Qiuyan
Q
Department of Nutritional Sciences, The Pennsylvania State University, 126-S Henderson Building, University Park, PA 16802, USA.
Ross
A Catharine
AC
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D019013:CD40 Antigens; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation; D017578:Immunoglobulin Class Switching; D007074:Immunoglobulin G / Q000235:genetics / Q000378:metabolism*; D015847:Interleukin-4; D008297:Male; D051379:Mice; D009700:Nucleoside Deaminases / Q000235:genetics / Q000378:metabolism; D000074462:Positive Regulatory Domain I-Binding Factor 1; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000276:immunology*; D053668:Syndecan-1 / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014212:Tretinoin / Q000276:immunology*
D019013:CD40 Antigens; D007074:Immunoglobulin G; C513258:Prdm1 protein, mouse; D012097:Repressor Proteins; D053668:Syndecan-1; D014157:Transcription Factors; D015847:Interleukin-4; D014212:Tretinoin; D000074462:Positive Regulatory Domain I-Binding Factor 1; D009700:Nucleoside Deaminases