title: TOF-SIMS and principal component analysis characterization of the multilayer surface grafting of small molecules: antibacterial furanones.
abstract: Furanone compounds (fimbrolides) have attracted interest as antibacterial compounds for use in human health care, for instance, as an antibacterial coating for medical devices to combat device-centered infections. To ensure effectiveness for extended periods of time, they must be immobilized covalently onto a device surface; in this study, this was done via azide/nitrene chemistry and photochemical coupling. However, the detection and quantification of surface-immobilized small molecules such as furanones presents a considerable analytical challenge, yet is necessary for optimization of coatings and reliable interpretation of biological responses. We have utilized the surface sensitivity and chemical specificity of time-of-flight secondary ion mass spectrometry (TOF-SIMS) to characterize each step of the grafting sequence. On account of the complexity of the data, principal component analysis (PCA) was used to interpret and compare spectra. The results demonstrate the utility of TOF-SIMS with PCA for the detection of the surface-grafted small molecules azidoaniline and a brominated furanone; imaging of the bromine ion peaks also enabled assessment of grafting uniformity. Thus, successful multilayer coating and furanone grafting was observed, and substantial and uniform coverage of furanone molecules on the surface. Even multiple grafting steps involving, in the present case, two low molecular weight compounds can readily be disentangled by PCA. The utility of TOF-SIMS analysis with PCA is particularly well illustrated in the present case by the grafting of the furanone molecules, which did not yield a singular unique peak in the positive ion mass spectra, whereas the collective spectral changes elucidated by PCA provided unambiguous verification of successful grafting of this low molecular weight compound.
authors: Ian Wark Research Institute, University of South Australia, Mawson Lakes, SA 5095, Australia. sameer.al-bataineh@mat.ethz.ch
authors: Sameer A
authors: SA
authors: Al-Bataineh
authors: Marek
authors: M
authors: Jasieniak
authors: Leanne G
authors: LG
authors: Britcher
authors: Hans J
authors: HJ
authors: Griesser
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001386:Azides / Q000737:chemistry; D005663:Furans / Q000737:chemistry*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D025341:Principal Component Analysis
chemical_list: D000900:Anti-Bacterial Agents; D001386:Azides; D005663:Furans; D007202:Indicators and Reagents