Cloth-based hybridization array system for expanded identification of the animal species origin of derived materials in feeds.
A cloth-based hybridization array system (CHAS) previously developed for the detection of animal species for which prohibited materials have been specified (cattle, sheep, goat, elk, and deer) has been expanded to include the detection of animal species for which there are no prohibitions (pig and horse) in Canadian and American animal feeds. Animal species were identified by amplification of mitochondrial DNA sequences by PCR and subsequent hybridization of the amplicons with an array of species-specific oligonucleotide capture probes immobilized on a polyester cloth support, followed by an immunoenzymatic assay of the bound PCR products. The CHAS permitted sensitive and specific detection of meat meals from different animal species blended in a grain-based feed and should provide a useful adjunct to microscopic examination for the identification of prohibited materials in animal feeds.
Murphy
Johanna
J
Ottawa Laboratory (Carling), Canadian Food Inspection Agency, Ottawa, Ontario, Canada K1A 0C6.
Armour
Jennifer
J
Blais
Burton W
BW
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle; D004272:DNA, Mitochondrial / Q000302:isolation & purification*; D003670:Deer; D005506:Food Contamination / Q000032:analysis*; D006041:Goats; D006736:Horses; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012756:Sheep; D013045:Species Specificity; D013552:Swine
D004272:DNA, Mitochondrial