Development and evaluation of serological assays for detection of Hantaanvirus-specific antibodies in human sera using yeast-expressed nucleocapsid protein.
Indirect and capture enzyme-linked immunosorbent assays (ELISAs) for detection of Hantaan virus (HTNV)-specific immunoglobulins G (IgG) and M (IgM) in human serum samples were developed on the basis of recombinant yeast-expressed nucleocapsid (N) protein of HTNV. The sensitivities and specificities of the indirect and capture ELISAs were evaluated by comparing the reactivity of sera from patients with hemorrhagic fever with renal syndrome (HFRS) from China with that of a commercial IgG/IgM kit. The sensitivity of the indirect IgG and IgM ELISA tests was both 100% and the specificity of the indirect IgM and IgG ELISA test was 98% and 99%, respectively. The sensitivity and specificity of the capture IgM ELISA was 100% and 97%, respectively. The novel assays were found to detect HTNV-specific antibodies in acute phase sera from suspected HFRS patients in China. The results indicate that these novel ELISAs are suitable for the diagnosis of HTNV and for sero-epidemiological studies.
Petraityte
Rasa
R
Institute of Biotechnology, VA Graiciūno 8, LT-02241, Vilnius, Lithuania.
Yang
Hua
H
Hunjan
Rashpal
R
Razanskiene
Ausra
A
Dhanilall
Pravesh
P
Ulrich
Rainer G
RG
Sasnauskas
Kestutis
K
Jin
Li
L
D000914:Antibodies, Viral / Q000097:blood*; D036022:Capsid Proteins / Q000235:genetics; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D018063:Hantaan virus / Q000276:immunology / Q000302:isolation & purification*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000175:diagnosis*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D011994:Recombinant Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012680:Sensitivity and Specificity; D014758:Viral Core Proteins / Q000235:genetics
D000914:Antibodies, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011994:Recombinant Proteins; D014758:Viral Core Proteins; C111782:nucleocapsid protein, Hantaan virus