Development of artificial restriction DNA cutter composed of Ce(Iv)/EDTA and PNA.
An artificial restriction enzyme, which we developed recently by combining Ce(IV)/EDTA and peptide nucleic acids, was used for PCR-free construction of a fusion protein. The fusion protein was successfully expressed in mammalian cells. This artificial DNA cutter can be also applied to site-selective scission of huge DNAs. Promising features of this novel tool were concretely evidenced.
Yamamoto
Yoji
Y
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Muguro-ku, Tokyo, Japan.
Uehara
Akihiko
A
Miura
Kazuyuki
K
Watanabe
Akira
A
Aburatani
Hiroyuki
H
Komiyama
Makoto
M
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D002563:Cerium / Q000737:chemistry*; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry*; D053837:DNA Cleavage; D004262:DNA Restriction Enzymes / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000235:genetics
D004279:DNA, Viral; D020135:Peptide Nucleic Acids; D011993:Recombinant Fusion Proteins; D002563:Cerium; D004247:DNA; D004492:Edetic Acid; D004262:DNA Restriction Enzymes