title: Impact of intense pulse light irradiation on BALB/c mouse skin-in vivo study on collagens, matrix metalloproteinases and vascular endothelial growth factor.
abstract: Our aim was to investigate the effects of intense pulsed light (IPL) on collagen expression in BALB/c mouse skin and confirm its relative molecular mechanisms. The dorsal skin of BALB/c mice was irradiated by IPL. Before treatment and from 1 day to 8 weeks (1 day, 3 days, 5 days, 1 week, 2 weeks, 3 weeks, 4 weeks, 6 weeks and 8 weeks) after treatment, the irradiated skin specimens were examined. The histology showed dermis thickening, accompanied with increased collagen and better organization. After IPL irradiation from 2 W up to 8 W, the staining of collagen types I and III in the IPL-treated groups was stronger than in the sham groups (P < 0.05), and the mRNA expression levels of the procollagen types I and III had also increased (P < 0.05). The up-regulation effects of IPL irradiation were time-dependent. The mRNA expression levels of matrix metalloproteinase (MMP)-1 and MMP-2 decreased progressively after IPL irradiation at 2 W up to 8 W (P < 0.05), and this down-regulation effect of IPL was also time-dependent. However, the mRNA expression of vascular endothelial growth factor (VEGF) had shown no obvious change by the end of the experiment (P > 0.05). Taking these factors together, we can conclude that IPL irradiation can not only enhance new collagen production, but also decrease collagen degradation in photo-rejuvenation mechanisms in mouse skin.
authors: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, PR China. daniluo2005@yahoo.com.cn
authors: Dan
authors: D
authors: Luo
authors: Yan
authors: Y
authors: Cao
authors: Di
authors: D
authors: Wu
authors: Yang
authors: Y
authors: Xu
authors: Bin
authors: B
authors: Chen
authors: Zhuyun
authors: Z
authors: Xue
mesh_terms: D000818:Animals; D003094:Collagen / Q000032:analysis*; D005260:Female; D008027:Light; D020781:Matrix Metalloproteinase 1 / Q000032:analysis; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D020782:Matrix Metalloproteinases / Q000032:analysis* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D011347:Procollagen / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012867:Skin / Q000737:chemistry / Q000528:radiation effects*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis* / Q000235:genetics
chemical_list: D011347:Procollagen; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D003094:Collagen; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1