title: Nesfatin-1 influences the excitability of paraventricular nucleus neurones.
abstract: Nesfatin-1 is a newly-discovered satiety peptide found in several nuclei of the hypothalamus, including the paraventricular nucleus. To begin to understand the physiological mechanisms underlying these satiety-inducing actions, we examined the effects of nesfatin-1 on the excitability of neurones in the paraventricular nucleus. Whole-cell current-clamp recordings from rat paraventricular nucleus neurones showed nesfatin-1 to have either hyperpolarizing or depolarising effects on the majority of neurones tested. Both types of response were observed in neurones irrespective of classification based on electrophysiological fingerprint (magnocellular, neuroendocrine or pre-autonomic) or molecular phenotype (vasopressin, oxytocin, corticotrophin-releasing hormone, thyrotrophin-releasing hormone or vesicular glutamate transporter), determined using single cell reverse transcription-polymerase chain reaction. Consequently, we provide the first evidence that this peptide, which is produced in the paraventricular nucleus, has effects on the membrane potential of a large proportion of different subpopulations of neurones located in this nucleus, and therefore identify nesfatin-1 as a potentially important regulator of paraventricular nucleus output.
authors: Department of Physiology, Queen's University, Kingston, Ontario, Canada.
authors: C J
authors: CJ
authors: Price
authors: T D
authors: TD
authors: Hoyda
authors: W K
authors: WK
authors: Samson
authors: A V
authors: AV
authors: Ferguson
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D004268:DNA-Binding Proteins; D066298:In Vitro Techniques; D008297:Male; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D000080866:Nucleobindins; D010121:Oxytocin / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism; D014667:Vasopressins / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; C000630652:Nucb1 protein, rat; D000080866:Nucleobindins; D014667:Vasopressins; D010121:Oxytocin; D013973:Thyrotropin-Releasing Hormone; D003346:Corticotropin-Releasing Hormone