title: Alu elements mediate MYB gene tandem duplication in human T-ALL.
abstract: Recent studies have demonstrated that the MYB oncogene is frequently duplicated in human T cell acute lymphoblastic leukemia (T-ALL). We find that the human MYB locus is flanked by 257-bp Alu repeats and that the duplication is mediated somatically by homologous recombination between the flanking Alu elements on sister chromatids. Nested long-range PCR analysis indicated a low frequency of homologous recombination leading to MYB tandem duplication in the peripheral blood mononuclear cells of approximately 50% of healthy individuals, none of whom had a MYB duplication in the germline. We conclude that Alu-mediated MYB tandem duplication occurs at low frequency during normal thymocyte development and is clonally selected during the molecular pathogenesis of human T-ALL.
authors: Department of Pediatric Oncology, Belfer Foundation Institute for Innovative Cancer Science, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
authors: Jennifer
authors: J
authors: O'Neil
authors: Joelle
authors: J
authors: Tchinda
authors: Alejandro
authors: A
authors: Gutierrez
authors: Lisa
authors: L
authors: Moreau
authors: Richard S
authors: RS
authors: Maser
authors: Kwok-Kin
authors: KK
authors: Wong
authors: Wei
authors: W
authors: Li
authors: Keith
authors: K
authors: McKenna
authors: X Shirley
authors: XS
authors: Liu
authors: Bin
authors: B
authors: Feng
authors: Donna
authors: D
authors: Neuberg
authors: Lewis
authors: L
authors: Silverman
authors: Daniel J
authors: DJ
authors: DeAngelo
authors: Jeffery L
authors: JL
authors: Kutok
authors: Rodney
authors: R
authors: Rothstein
authors: Ronald A
authors: RA
authors: DePinho
authors: Lynda
authors: L
authors: Chin
authors: Charles
authors: C
authors: Lee
authors: A Thomas
authors: AT
authors: Look
mesh_terms: D020087:Alu Elements / Q000235:genetics*; D001483:Base Sequence; D020440:Gene Duplication; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics*; D008954:Models, Biological; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics*; D011995:Recombination, Genetic
chemical_list: D020598:Proto-Oncogene Proteins c-myb