title: Impaired nerve regeneration in reeler mice after peripheral nerve injury.
abstract: Reelin, an extracellular matrix protein, plays an important role in the regulation of neuronal migration and cortical lamination in the developing brain. Little is known, however, about the role of this protein in axonal regeneration. We have previously shown that Reelin is secreted by Schwann cells in the peripheral nerve compartment during postnatal development and that it is up-regulated following nerve injury in adult mice. In this work, we generated mice deficient in Reelin (reeler) that express yellow fluorescent protein (YFP) in a subset of neurons and examined the axonal regeneration following nerve crush. We found that axonal regeneration was significantly altered compared with wild-type mice. By contrast, retrograde tracing with Fluorogold dye after sciatic nerve crush was unaffected in these mutants, being comparable with normal axonal transport observed in wild-type. These results indicate that the absence of Reelin impairs axonal regeneration following injury and support a role for this protein in the process of peripheral nerve regeneration.
authors: Dipartimento di Scienze Neurologiche e della Visione, Università di Verona, Strada Le Grazie n.8, 37134 Verona, Italy.
authors: Erika
authors: E
authors: Lorenzetto
authors: Roger
authors: R
authors: Panteri
authors: Ramona
authors: R
authors: Marino
authors: Flavio
authors: F
authors: Keller
authors: Mario
authors: M
authors: Buffelli
mesh_terms: D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000172:deficiency / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000172:deficiency / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology*; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000378:metabolism*; D010523:Peripheral Nervous System Diseases / Q000235:genetics* / Q000503:physiopathology*; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000172:deficiency / Q000378:metabolism*; D013266:Stilbamidines / Q000378:metabolism
chemical_list: C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D008164:Luminescent Proteins; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; D013266:Stilbamidines; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases