Diphenyl diselenide supplementation delays the development of N-nitroso-N-methylurea-induced mammary tumors.
The effect of dietary diphenyl diselenide (1 ppm) on N-nitroso-N-methylurea (NMU)-induced mammary carcinogenesis was examined in female Wistar rats. Beginning at 5 weeks of age, the animals were fed with either control or diphenyl-diselenide-supplied diets until the end of the study (210 days). At 50 days of age, mammary tumor was induced by the administration of three doses of NMU (50 mg/kg body wt, intraperitoneally) once a week for 3 weeks. In experimental trials, latency to tumor onset was extended in rats fed with diet supplemented with diphenyl diselenide (P < 0.05). The incidence and frequency of tumors were significantly small in animals supplemented with diphenyl diselenide. However, the multiplicity of tumors was not altered by dietary diphenyl diselenide. Diphenyl diselenide supplementation also restored superoxide dismutase (SOD) activity and vitamin C levels altered in the NMU group (P < 0.05). Our results suggest that diphenyl diselenide can be considered a chemopreventive agent, even when supplemented at a relatively low concentration.
de Vargas Barbosa
Nilda Berenice
NB
Centro de Ciências da Saúde, Universidade Federal de Santa Maria-UNIPAMPA, Rua Domingos de Almeida, São Miguel, Uruguaiana, RS, Brazil. nvbarbosa@yahoo.com.br
Nogueira
Cristina Wayne
CW
Guecheva
Temenouga N
TN
Bellinaso
Maria de Lourdes
Mde L
Rocha
João Batista Teixeira
JB
D000818:Animals; D016588:Anticarcinogenic Agents; D001205:Ascorbic Acid / Q000378:metabolism; D001555:Benzene Derivatives / Q000494:pharmacology*; D015415:Biomarkers; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D020552:Comet Assay; D004032:Diet; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D015674:Mammary Neoplasms, Animal / Q000139:chemically induced* / Q000517:prevention & control*; D008770:Methylnitrosourea / Q000633:toxicity*; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D009929:Organ Size / Q000187:drug effects; D016566:Organoselenium Compounds / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D016588:Anticarcinogenic Agents; D001555:Benzene Derivatives; D015415:Biomarkers; D002273:Carcinogens; D009363:Neoplasm Proteins; D016566:Organoselenium Compounds; C061132:diphenyldiselenide; D008770:Methylnitrosourea; D013482:Superoxide Dismutase; D001205:Ascorbic Acid