Phenotypes and genotypes of campylobacter strains isolated after cleaning and disinfection in poultry slaughterhouses.
Campylobacter is responsible for human bacterial enteritis and poultry meat is recognised as a primary source of infection. In slaughterhouses, cleaning and disinfection procedures are performed daily, and it has been suggested that disinfectant molecules might select for antibiotic resistant strains if shared targets or combined resistance mechanisms were involved. The aim of the study was to investigate if cleaning and disinfection procedures in poultry slaughterhouses select for antibiotic resistance in Campylobacter jejuni and C. coli and to determine the genotypes of isolates collected after cleaning and disinfection. Nine sampling visits were made to four French slaughterhouses. Samples were collected from transport crates and equipment surfaces, before and after cleaning and disinfection. Minimal inhibitory concentrations of the recovered C. jejuni and C. coli isolates to six antibiotics and two disinfectants were measured. The C. jejuni isolates collected from equipment surfaces after cleaning and disinfection were subjected to PCR-RFLP typing. Twenty-five C. jejuni isolates and 1 C. coli were recovered from equipment surfaces after cleaning and disinfection during five visits to three different slaughterhouses. Those isolates did not show an increased resistance to the tested antibiotics compared to isolates collected before cleaning and disinfection. Only one or two genotypes were recovered after cleaning and disinfection during single visits to each slaughterhouse. This observation suggests that such genotypes may be particularly adapted to survive cleaning and disinfection stress. Understanding the survival mechanisms of Campylobacter should facilitate the implementation of better-targeted strategies and reduce the public health burden associated with Campylobacter infection.
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Agence française de sécurité sanitaire des aliments (AFSSA), Laboratoire d'études et de recherches sur les médicaments vétérinaires et les désinfectants. BP 90203 La Haute Marche, 35302 Fougères Cedex, France.
Soumet
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Sanders
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D000003:Abattoirs; D000222:Adaptation, Physiological; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002167:Campylobacter / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000188:drug therapy / Q000635:transmission / Q000662:veterinary*; D017000:Campylobacter coli / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D015169:Colony Count, Microbial / Q000662:veterinary; D003257:Consumer Product Safety; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004866:Equipment Contamination / Q000517:prevention & control; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control; D005516:Food Microbiology; D005602:France / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D006920:Hygiene; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D011200:Poultry; D011201:Poultry Diseases / Q000188:drug therapy* / Q000635:transmission; D015995:Prevalence; D011634:Public Health; D013045:Species Specificity
D000890:Anti-Infective Agents