title: Isolation and characterization of the full coding sequence of a novel densovirus from the mosquito Culex pipiens pallens.
abstract: During an investigation of arboviruses in China, a novel densovirus (DNV) was isolated from the adult female Culex pipiens pallens. The virus, designated Culex pipiens pallens densovirus (CppDNV), caused cytopathic effect in C6/36 cells. The virus particles were icosahedral, non-enveloped and had a mean diameter of 24 nm. The complete coding region of CppDNV was found to be 3335 nt and it contained three open reading frames (ORFs). CppDNV shares 82-93 % identical nucleotides with isolates of the Aedes albopictus densovirus [isolates AalDNV-1, AalDNV-2 (C6/36 DNV) and AalDNV-3], Aedes aegypti densovirus (AaeDNV) and Haemagogus equines densovirus (HeDNV). The nucleotide sequence identity among CppDNV isolates exceeds 98 %. Phylogenetic trees based on non-structural (NS1 and NS2) and capsid (VP) genes show that CppDNV clustered with the species AaeDNV and represents a novel variant of this species within the genus Brevidensovirus.
authors: State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 Ying Xin Street, Beijing 100052, PR China.
authors: You-Gang
authors: YG
authors: Zhai
authors: State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 Ying Xin Street, Beijing 100052, PR China.
authors: Xin-Jun
authors: XJ
authors: Lv
authors: State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 Ying Xin Street, Beijing 100052, PR China.
authors: Xiao-Hong
authors: XH
authors: Sun
authors: State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 Ying Xin Street, Beijing 100052, PR China.
authors: Shi-Hong
authors: SH
authors: Fu
authors: Department of Arbovirus and Chlamydia, Yunnan Institute of Endemic Diseases Control and Prevention, 33 Wenhua Road, Dali City 67100, Yunnan Province, PR China.
authors: Zheng-da
authors: ZD
authors: Gong
authors: Department of Arbovirus and Chlamydia, Yunnan Institute of Endemic Diseases Control and Prevention, 33 Wenhua Road, Dali City 67100, Yunnan Province, PR China.
authors: Yun
authors: Y
authors: Fen
authors: Xinjiang Center for Disease Control and Prevention, 1 Jianquan Street, Urumqi City 830000, Xinjiang Uygur autonomous, PR China.
authors: Su-Xiang
authors: SX
authors: Tong
authors: Department of Virology, Guizhou Province Center for Disease Control and Prevention, 73 BaGeYan Road, GuiYang City 550004, Guizhou Province, PR China.
authors: Zhao-Xiao
authors: ZX
authors: Wang
authors: State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 Ying Xin Street, Beijing 100052, PR China.
authors: Qing
authors: Q
authors: Tang
authors: Department of Arbovirology, Institute for Animal Health, Pirbright, Woking, Surrey GU24 0NF, UK.
authors: Houssam
authors: H
authors: Attoui
authors: State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 Ying Xin Street, Beijing 100052, PR China.
authors: Guo-Dong
authors: GD
authors: Liang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002681:China; D017124:Conserved Sequence; D003465:Culex / Q000821:virology*; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D017990:Densovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D017931:DNA Primers; D004279:DNA, Viral