Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin.
Escherichia coli O157:H7 produces Stx1 and Stx2 causing severe diseases. Their B subunits (StxBs) are useful for a vaccine but exhibit low immunogenicity, especially Stx2B. Nasal vaccination with StxBs plus cholera toxin induces only serum anti-Stx1B antibodies in mice. However, nasal administration of a mutant of E. coli enterotoxin and His-tagged Stx2B induced serum antibodies neutralizing Stx2 in vitro or in vivo and mucosal IgA antibodies in lungs. As His-tagged Stx2B showed five or three polymers in gel filtration chromatography, His-tagged Stx2B forms smaller tertiary structure than the native one and is effective for preventing Stx2 toxemia as a nasal vaccine.
Tsuji
Takao
T
Department of Microbiology, Fujita Health University, School of Medicine, Toyoake, Aichi, Japan. ttsuji@fujita-hu.ac.jp
Shimizu
Takeshi
T
Sasaki
Keiko
K
Shimizu
Yoshiyasu
Y
Tsukamoto
Kentaro
K
Arimitsu
Hideyuki
H
Ochi
Sadayuki
S
Sugiyama
Satoshi
S
Taniguchi
Koki
K
Neri
Paola
P
Mori
Hiroshi
H
D000276:Adjuvants, Immunologic; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology; D000918:Antibody Specificity; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology*; D002850:Chromatography, Gel; D004768:Enterotoxins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology*; D005260:Female; D006639:Histidine; D007114:Immunization; D007115:Immunization Schedule; D007070:Immunoglobulin A / Q000032:analysis; D008168:Lung / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D009092:Mucous Membrane / Q000276:immunology; D009154:Mutation; D009500:Neutralization Tests; D011108:Polymers; D021122:Protein Subunits / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D022641:Shiga Toxin 2 / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D014115:Toxemia / Q000517:prevention & control*; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D004768:Enterotoxins; D007070:Immunoglobulin A; D011108:Polymers; D021122:Protein Subunits; D022641:Shiga Toxin 2; D022223:Vaccines, Subunit; D006639:Histidine; D002772:Cholera Toxin