[Transcription of genes of membrane proteins of Brazilians isolates of Anaplasma marginale].
This work shows the transcription profile of membrane protein genes in three Brazilian isolates of Anaplasma marginale (Rio Grande do Norte, Pernambuco-Zona da Mata, and Pernambuco-Sertão). RNA was purified from cattle blood experimentally-infected with the three isolates of A. marginale. After reverse transcription, genes omp1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, and 14; opag1-3; virB3, 9, and 10; am097, 197, 254, 854, and 956 were amplified by PCR, with specific primers. Transcripts were detected for all genes, except omp2, 3 e opag3 in all isolates and for omp7 in one out of the three isolates analyzed. Absence of transcription for opag3 and omp7 diverge from the North American isolates of A. marginale. Reasons for such differences were discussed.
Ramos
Carlos A N
CA
Programa de Pós-Graduação em Ciência Animal, Universidade Federalde Mato Grosso do Sul, Cidade Universitária, Caixa Postal 549, CampoGrande, MS 79070-900.
Araújo
Flábio R
FR
Osório
Ana L A R
AL
Madruga
Cláudio R
CR
Rosinha
Grácia M S
GM
Soares
Cleber O
CO
Elisei
Carina
C
D042322:Anaplasma marginale / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001938:Brazil; D002417:Cattle / Q000382:microbiology; D014158:Transcription, Genetic
D001425:Bacterial Outer Membrane Proteins