[Effect on induction of differentiation of Tca8113 cells affected by abscisic acid in vitro].
OBJECTIVE
To approach the effects on induction of differentiation of Tca8113 cells affected by abscisic acid.


METHODS
The changes of surface differentiation markers, cell configuration, restrain of cell growth and the expression of Caspase-3 mRNA were examined by using inverted-phase contrast microscope, immunohistochemistry (IHC) and in situ hybridization in vitro. The dependablity between the surface differentiation markers and Caspase-3 mRNA was analysed.


RESULTS
The restraint of cell growth in ABA groups was higher than that of the control group (P<0.05). There was a trend that the tumor cell had transformed the normal cell. Furthermore, the time-dosage dependent relationship existed in the inhibition rate of tumor cells. The results showed that the expressions of Involucrin protein, retinoic acid receptor beta (RARbeta) and Caspase-3 mRNA in experimental group had been higher than that of control group. There was a significance between the different concentration experimental groups at 24 h (P<0.05). Moreover, the positive correlation existed among the Involucrin, RARbeta and Caspase-3 mRNA at the time of 12 hour and 24 hour (P<0.05).


CONCLUSIONS
The possible mechanism is that abscisic acid acted on the tumor cell and raised the level of RARbeta gene through combining the correlative receptors so that increased the expression of Involucrin protein and promoted the activity of Caspase-3 and resulted in apoptosis of tumor cell.
Zhao
Hong-wei
HW
Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu, China.
Li
Long-jiang
LJ
Pan
Jian
J
Han
Bo
B
Wen
Yu-ming
YM
D000040:Abscisic Acid; D017209:Apoptosis; D002454:Cell Differentiation; D002455:Cell Division; D049109:Cell Proliferation; D066298:In Vitro Techniques; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid
D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; C082925:retinoic acid receptor beta; D000040:Abscisic Acid