The importance of using multiple techniques for detection of platelet antibodies.
In red cell immunology, it has long been known that no one technique will detect all clinically significant antibodies. The same appears to be true for platelet immunology, and we highlight this fact by showing four examples of anti-human platelet antigen-1a that were not detected by the monoclonal antibody-specific immobilization of platelet antigen test, the most commonly used technique. Each antibody was found in a case of fetomaternal alloimmune thrombocytopenia in which the fetus or neonate was severely affected.
Smith
G A
GA
Platelet Immunology Reference Laboratory, National Health Service Blood and Transplant, Cambridge, UK.
Ranasinghe
E
E
Ouwehand
W H
WH
D016824:Antigens, Human Platelet / Q000097:blood / Q000276:immunology*; D000094902:Blood Banking / Q000379:methods; D001792:Blood Platelets / Q000276:immunology*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D039661:Integrin beta3; D007518:Isoantibodies / Q000009:adverse effects / Q000032:analysis* / Q000276:immunology; D017713:Platelet Transfusion; D011247:Pregnancy; D054098:Thrombocytopenia, Neonatal Alloimmune / Q000097:blood / Q000175:diagnosis*
D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D039661:Integrin beta3; D007518:Isoantibodies