[PDCD5 induces the apoptosis of human prostate cancer cells PC-3M-1E8].
OBJECTIVE
To investigate the apoptosis-promoting effect of PDCD5 on human prostate cancer cells PC-3M-1E8.


METHODS
PCI-neo and PCI-neo-PDCD5 were transfected into PC-3M-1E8 cells by Lipofectamine 2000, the viability of the cells was analyzed by MTT assay 16 hours after removal of the serum, and the apoptosis was determined by in situ end-labeling and electron microscopy.


RESULTS
The viability and growing speed of the transfected cells were significantly decreased and their apoptotic indexes significantly increased as compared with the control group (P < 0.001).


CONCLUSIONS
PDCD5 may significantly inhibit the in vitro growth and promote the apoptosis of human prostate cancer cells PC-3M-1E8.
Li
Shu-jun
SJ
Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongiiang 150081, China.
Yu
Jing
J
Zhao
Xue-fei
XF
Jiang
Ying
Y
Liu
Zi-jun
ZJ
Yu
Xiao-guang
XG
D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000502:physiology*; D045744:Cell Line, Tumor; D006801:Humans; D020287:In Situ Nick-End Labeling; D008055:Lipids / Q000737:chemistry; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics / Q000502:physiology*; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection / Q000379:methods
D051017:Apoptosis Regulatory Proteins; D008055:Lipids; C086724:Lipofectamine; D009363:Neoplasm Proteins; C117283:PDCD5 protein, human