Detection of terminal mismatches on DNA duplexes in homogeneous assays or with immobilized probes.
We recently reported the design of new fluorescent oligo-2'-deoxyribonucleotides (FODNs) for the detection of terminal mismatches on DNA duplexes in homogeneous assays. We now report the validation of this method in homogeneous assays with other sequences and the feasibility of the detection of terminal mismatches with immobilized FODNs. In all cases studied, the mismatched duplexes were more fluorescent than the perfect ones and results confirmed that the discrimination factor is sequence-dependent.
Chassignol
Marcel
M
Centre de Biophysique Moléculaire, UPR 4301 CNRS affiliated with the University of Orléans and with INSERM, rue Charles Sadron, 45071 Orléans, France.
Aubert
Yves
Y
Roig
Victoria
V
Asseline
Ulysse
U
D020137:Base Pair Mismatch; D004252:DNA Mutational Analysis / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*
D015342:DNA Probes; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes