Glycopolydiacetylene nanoparticles as a chromatic biosensor to detect Shiga-like toxin producing Escherichia coli O157:H7.
Shiga toxin-producing Escherichia coli organisms (STEC) were detected by Gal-alpha1,4-Gal glycopolydiacetylene (GPDA) nanoparticles through the selective binding between Shiga toxin and GPDA nanoparticles. The binding produced a colorimetric change in the absorption wavelength of the GPDA nanoparticles. This method provides a highly selective, rapid, sensitive, and quantitative approach for the detection of STEC.
Nagy
Jon O
JO
ATERIS Technologies, Bozeman, MT 59718, USA. wang.892@osu.edu
Zhang
Yalong
Y
Yi
Wen
W
Liu
Xianwei
X
Motari
Edwin
E
Song
Jing Catherine
JC
Lejeune
Jeffrey T
JT
Wang
Peng George
PG
D015374:Biosensing Techniques; D019453:Escherichia coli O157 / Q000302:isolation & purification* / Q000378:metabolism; D053758:Nanoparticles; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; D022603:Shiga Toxins / Q000096:biosynthesis*
D000078789:Polyacetylene Polymer; D011108:Polymers; D022603:Shiga Toxins; D053279:Polyynes; C082361:polydiacetylene