Staged-injection procedure to prevent cement leakage during vertebroplasty: an in vitro study.
METHODS
Fibrin sealant (FS) combined with or without growth factor was used to improve the micro-architectural and biomechanical properties of vertebral body in osteoporotic ovine spine.


OBJECTIVE
To analyze the treatment effects of bovine bone morphogenetic protein (bBMP) combined with FS on osteopenic ovine vertebral architecture, bone mineral density, and biomechanics in vivo.


BACKGROUND
Vertebroplasty and kyphoplasty were used to treat spinal osteoporosis. They can increase strength of vertebrae physically. However, each has specific disadvantages. bBMP could rapidly increasing bone formation and suppressing bone resorption, but little is known about its effect on ovariectomized-induced osteoporosis.


METHODS
Six sheep underwent ovariectomy and were placed on a low-calcium diet. Twelve months later, according to Ladin square design, L4-L6 vertebrae in all sheep were randomly assigned to 3 treatment groups: A (30 mg bBMP/1.5 mL FS), B (30 mg bBMP) and C (1.5 mL FS). All materials were injected into the assigned vertebra transpedicularly. Animals were killed 3 months after injection, and bone mineral density (BMD), biomechanics, and histomorphometry were assessed. Analysis of variance was used to determine effects of bBMP/FS (alpha = 0.05).


RESULTS
The BMD in Group 1 was 17.1% and 14.7% higher than that in Group 2 and Group 3, respectively. The micro-CT reconstruction analysis showed that the density and connectivity of trabecular bone in bBMP/FS treated vertebrae were higher than those in control groups. The mechanical properties (yield stress, ultimate stress, energy absorption, bone modulus) of the vertebrae were also significantly higher. In this study, local bBMP/FS treatment showed a positive trend in improving BMD, histomorphometric parameters, and mechanical strength of osteoporotic vertebra. Slow release of bBMP using FS appeared to be an effective method of protein delivery.


CONCLUSIONS
The use of bBMP/FS in the treatment of vertebral osteoporosis in an attempt to enhance bone strength merits further study.
Wu
Zi-xiang
ZX
Institude of Orthopaedics, Xijing Hospital, the Fourth Military Medical University, Xi'an Shaanxi Province, PR China.
Wei
Lei
L
Hu
Yun-yu
YY
Wang
Hai-qiang
HQ
Wan
Shi-yong
SY
Wang
Jun
J
Han
Yisheng
Y
D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001846:Bone Development / Q000187:drug effects; D019485:Bone Morphogenetic Proteins / Q000627:therapeutic use*; D001862:Bone Resorption / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control; D004195:Disease Models, Animal; D004334:Drug Administration Schedule; D005260:Female; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D006801:Humans; D007267:Injections; D010012:Osteogenesis / Q000187:drug effects; D015663:Osteoporosis, Postmenopausal / Q000188:drug therapy* / Q000473:pathology / Q000601:surgery*; D034561:Sheep, Domestic; D013131:Spine / Q000187:drug effects / Q000473:pathology / Q000601:surgery; D013314:Stress, Mechanical; D013718:Tensile Strength; D016896:Treatment Outcome; D054854:Vertebroplasty / Q000379:methods*
D019485:Bone Morphogenetic Proteins; D015718:Fibrin Tissue Adhesive