Proteomic analysis of RNA-binding proteins in dry seeds of rice after fractionation by ssDNA affinity column chromatography.
In proteomic analysis, one of the major limitations is the detection of low-abundance proteins. To detect low-abundance RNA-binding proteins in mature dry seeds of rice, fractionation by single stranded DNA (ssDNA) affinity column chromatography was carried out before analysis by two-dimensional gel electrophoresis (2-DE). Proteomic analysis of the ssDNA-binding fraction revealed the existence of three types of RNA-binding proteins, including a K homology (KH) domain containing protein, a putative RNA-binding protein and a glycine-rich RNA-binding protein, in mature seeds. In addition, decreases in the putative RNA-binding protein and glycine-rich RNA-binding protein after absorbing water in seeds appear to be associated with seed germination.
Masaki
Shunpei
S
Department of Plant Production United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, 183-8509, Tokyo, Japan.
Yamada
Tetsuya
T
Hirasawa
Tadashi
T
Todaka
Daisuke
D
Kanekatsu
Motoki
M
D005591:Chemical Fractionation; D002846:Chromatography, Affinity; D004277:DNA, Single-Stranded; D015180:Electrophoresis, Gel, Two-Dimensional; D012275:Oryza / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D040901:Proteomics; D016601:RNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014867:Water / Q000378:metabolism
D004277:DNA, Single-Stranded; D010940:Plant Proteins; D016601:RNA-Binding Proteins; D014867:Water