Probing the redox activity of T-lymphocytes deposited at electrode surfaces with voltammetric methods.
BACKGROUND
Reactive oxygen species and redox signaling play an important role in the regulation of many vital biological processes. However, they are also tightly connected with many pathological conditions. The detection and evaluation of these signaling events are very often accompanied with great difficulties. In this article, we describe the development of a novel electrochemically-based technique for monitoring the cellular redox state.


RESULTS
T-cells were attached on the surface of a working electrode, which was modified with 2-palmitoylhydroquinone as a redox mediator. Using cyclic voltammetry, we were able to indirectly (via the redox mediator) monitor an electron transport from the cells towards the working electrode, which enabled us to evaluate the redox activity of the cells.


CONCLUSIONS
This new technique is rather simple and sensitive and may be used in the future as a valid diagnostic procedure in various branches of biomedical science.
Bogeski
Ivan
I
Department of Physiology, Saarland University, Homburg, Germany. ivan.bogeski@uks.eu
Mirceski
Valentin
V
Hoth
Markus
M
D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006801:Humans; D019169:Jurkat Cells; D010084:Oxidation-Reduction; D013601:T-Lymphocytes / Q000276:immunology*