title: [Selection of the microorganism for dioscin hydrolyze].
abstract: OBJECTIVE
  To select the microorganism which can hydrolyze dioscin to diosgenin.
  
  
  METHODS
  The microorganism were selected from the surface of rhizome, rhizosphere soil, the inside of the leaves and rhizome of Dioscorea zingiberensis C. H. Wright. Diosgenin was identified by thin-layer chromatography and HPLC.
  
  
  RESULTS
  The microorganism which could hydrolyze dioscin from the experiment were identified as Aspergillus sp and Alternaria sp. Characteristics of enzymes production and fermentation technology of Aspergillus No. 1 were also studied primarily.
  
  
  CONCLUSIONS
  The Aspergillus strain No. 1 can secret enzyme to hydrolyze dioscin into diosgenin effectively.
authors: College of Life Science, Nanjing Normal University, Nanjing 210046, China.
authors: Yu-ting
authors: YT
authors: Zhao
authors: Zeng-lai
authors: ZL
authors: Xu
authors: Chuan-chao
authors: CC
authors: Dai
authors: Xing-li
authors: XL
authors: Tang
authors: Bing
authors: B
authors: Xia
authors: Qiong
authors: Q
authors: Wang
mesh_terms: D000528:Alternaria / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D001230:Aspergillus / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D027742:Dioscorea / Q000382:microbiology*; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000378:metabolism; D006868:Hydrolysis; D010946:Plants, Medicinal / Q000382:microbiology*; D027343:Rhizome / Q000382:microbiology
chemical_list: C019357:dioscin; D004144:Diosgenin