Molecular method for Bartonella species identification in clinical and environmental samples.
A new, efficient molecular method for detection of Bartonella, based on the 16S-23S rRNA intergenic spacer and 16S rRNA amplification by multiplex PCR combined with reverse line blotting, was designed. This assay could simultaneously detect 20 different known species and other Bartonella species not described previously.
García-Esteban
Coral
C
Laboratorio de Espiroquetas y Patógenos Especiales, Servicio de Bacteriología, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain.
Gil
Horacio
H
Rodríguez-Vargas
Manuela
M
Gerrikagoitia
Xeider
X
Barandika
Jesse
J
Escudero
Raquel
R
Jado
Isabel
I
García-Amil
Cristina
C
Barral
Marta
M
García-Pérez
Ana L
AL
Bhide
Mangesh
M
Anda
Pedro
P
D001431:Bacteriological Techniques / Q000379:methods*; D001473:Bartonella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001474:Bartonella Infections / Q000175:diagnosis*; D017931:DNA Primers / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D021903:DNA, Ribosomal Spacer; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S