title: PCR-based specific detection of Ralstonia solanacearum by amplification of cytochrome c1 signal peptide sequences.
abstract: A polymerase chain reaction (PCR)-based method was developed to detect the DNA of Ralstonia solanacearum, the causal agent of bacterial wilt in various crop plants. One pair of primers (RALSF and RALSR), designed using cytochrome c1 signal peptide sequences specific to R. solanacearum, produced a PCR product of 932 bp from 13 isolates of R. solanacearum from several countries. The primer specificity was then tested using DNA from 21 isolates of Ralstonia, Pseudomonas, Burkholderia, Xanthomonas, and Fusarium oxysporum f. sp. dianthi. The specificity of the cytochrome c1 signal peptide sequences in R. solanacearum was further confirmed by a DNA-dot blot analysis. Moreover, the primer pair was able to detect the pathogen in artificially inoculated soil and tomato plants. Therefore, the present results indicate that the primer pair can be effectively used for the detection of R. solanacearum in soil and host plants.
authors: National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Korea.
authors: Man Jung
authors: MJ
authors: Kang
authors: Mi Hee
authors: MH
authors: Lee
authors: Jae Kyung
authors: JK
authors: Shim
authors: Sang Tae
authors: ST
authors: Seo
authors: Rosemary
authors: R
authors: Shrestha
authors: Min Seok
authors: MS
authors: Cho
authors: Jang Ho
authors: JH
authors: Hahn
authors: Dong Suk
authors: DS
authors: Park
mesh_terms: D003575:Cytochromes c1 / Q000235:genetics*; D018551:Solanum lycopersicum / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D021382:Protein Sorting Signals / Q000235:genetics*; D043368:Ralstonia solanacearum / Q000235:genetics / Q000302:isolation & purification*; D012988:Soil Microbiology
chemical_list: D021382:Protein Sorting Signals; D003575:Cytochromes c1