title: Lineage-specific transition of histone signatures in the killer cell Ig-like receptor locus from hematopoietic progenitor to NK cells.
abstract: The clonal distribution and stable expression of killer cell Ig-like receptor (KIR) genes is epigenetically regulated. To assess the epigenetic changes that occur during hemopoietic development we examined DNA methylation and chromatin structure of the KIR locus in early hemopoietic progenitor cells and major lymphocyte lineages. In hemopoietic progenitor cells, KIR genes exhibited the major hallmarks of epigenetic repression, which are dense DNA methylation, inaccessibility of chromatin to Micrococcus nuclease digest, and a repressive histone signature, characterized by strong H3K9 dimethylation and reduced H4K8 acetylation. In contrast, KIR genes of NK cells showed active histone signatures characterized by absence of H3K9 dimethylation and presence of H4K8 acetylation. Histone modifications correlated well with the competence of different lymphocyte lineages to express KIR; whereas H4K8 acetylation was high in NK and CD8+ T cells, it was almost absent in CD4+ T cells and B cells and, in the latter case, replaced by H3K9 dimethylation. In KIR-competent lineages, active histone signatures were also observed in silent KIR genes and in this case found in combination with dense DNA methylation of the promoter and nearby regions. The study suggests a two-step model of epigenetic regulation in which lineage-specific acquisition of euchromatic histone marks is a prerequisite for subsequent gene-specific DNA demethylation and expression of KIR genes.
authors: Institute for Transplantation Diagnostics and Cell Therapeutics, Heinrich Heine University, Düsseldorf, Germany.
authors: Simeon
authors: S
authors: Santourlidis
authors: Nina
authors: N
authors: Graffmann
authors: Julia
authors: J
authors: Christ
authors: Markus
authors: M
authors: Uhrberg
mesh_terms: D019070:Cell Lineage / Q000235:genetics; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology*; D008836:Micrococcal Nuclease / Q000737:chemistry; D011401:Promoter Regions, Genetic; D054340:Receptors, KIR / Q000235:genetics*
chemical_list: D006657:Histones; D054340:Receptors, KIR; D008836:Micrococcal Nuclease