Activation of chloride secretion via proteinase-activated receptor 2 in a human eccrine sweat gland cell line--NCL-SG3.
Proteinase-activated receptor 2 (PAR-2) has been shown to elicit secretion in a variety of secretory epithelial cells by the transepithelial movement of chloride ions across the apical membrane. However, it is not known whether these receptors are present and/or functional in the secretory epithelial cells of the human eccrine sweat gland. To investigate this possibility mRNA analysis, Ca2+ microspectrofluorimetry and the short circuit current (Isc) technique were used to quantify electrolyte transport in a cell line (NCL-SG3) derived from human eccrine sweat gland secretory epithelia. The results provided molecular and functional evidence of the presence of PAR-2 receptors in the NCL-SG3 cells and show that these receptors can activate transepithelial Cl- secretion possibly via Ca2+-activated Cl- channels.
Bovell
Douglas L
DL
Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow, UK.
Santic
Radmila
R
Kofler
Barbara
B
Hermann
Anton
A
Wilson
Darius
D
Corbett
Alistair
A
Lang
Roland
R
D000584:Amiloride / Q000494:pharmacology; D002034:Bumetanide / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002712:Chlorides / Q000378:metabolism*; D004439:Eccrine Glands / Q000166:cytology; D004594:Electrophysiology; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D017136:Ion Transport; D009842:Oligopeptides / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D044464:Receptor, PAR-2 / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D026941:Sodium Channel Blockers / Q000494:pharmacology; D049994:Sodium Potassium Chloride Symporter Inhibitors / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D002712:Chlorides; D009842:Oligopeptides; D012333:RNA, Messenger; D044464:Receptor, PAR-2; D026941:Sodium Channel Blockers; D049994:Sodium Potassium Chloride Symporter Inhibitors; C515807:seryl-leucyl-isoleucyl-glycyl-lysyl-valinamide; D002034:Bumetanide; D000584:Amiloride; D014357:Trypsin; D002118:Calcium