Immunophenotypic peculiarities of mobilized stem (CD34+) cells in blood from patients with severe spinal cord injury.
Immunophenotype of mobilized stem blood cells (CD34+) was studied in 29 patients with late post-traumatic spinal lesions. The CD34+ cells demonstrated different levels of expression of CD45, CD38, monomorphic determinants HLA-DR and gp130 epitopes. Most patients presented with a CD34+ cell fraction with no or low expression of common leukocytic antigen CD45. Only 2 patients had greater than 15 percent of HLA-DR-CD38- cells in the CD34+ fraction. A common transducer molecule of interleukin-6 family cytokines gp130 was expressed on stem (CD34+) cells in all the cases, 26 percent of the patients had an activated gp130 phenotype, i.e. a combination of C7+ and A1- epitopes.
Tupitsyn
N N
NN
State N.N.Blokhin Russian Cancer Research Center Affiliated to the Russian Academy of Medical Sciences, Moscow, Russian Federation. cannt@aha.ru
Yaryghin
V N
VN
Bryukhovetskiy
A S
AS
Grivtsova
L Yu
LY
Mentkevich
G L
GL
Dolgopolov
I S
IS
Zaitsev
A Yu
AY
Davydov
M I
MI
D051997:ADP-ribosyl Cyclase 1 / Q000276:immunology; D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology*; D050822:Cytokine Receptor gp130 / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005260:Female; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D013119:Spinal Cord Injuries / Q000097:blood* / Q000276:immunology* / Q000473:pathology; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism
D018952:Antigens, CD34; D000939:Epitopes; D006684:HLA-DR Antigens; D050822:Cytokine Receptor gp130; D051997:ADP-ribosyl Cyclase 1