title: The phototoxicity of aged human retinal melanosomes.
abstract: The purpose of this study was to determine whether an age-related increase in photoreactivity of human retinal melanosomes (MS) can cause phototoxicity to retinal pigment epithelium (RPE) cells. MS were isolated post mortem from young (20-30 years, young human melanosomes [YHMs]) and old (60-90 years, old human melanosomes [OHMs]) human eyes and from young bovine eyes (bovine melanosomes [BMs]). Confluent cultured ARPE-19 cells were fed equivalent numbers of OHMs or BMs and accumulated similar amounts of melanin as determined by electron paramagnetic resonance assay. Cells with and without MS were either maintained in the dark or exposed to blue light for up to 96 h and assessed for alterations in cell morphology, cell viability and lysosomal integrity. Incubation of cells in dark in the presence of internalized MS or irradiation of cells with blue light in the absence or presence of BMs did not significantly affect cell viability. However, exposures to blue light in the presence of OHMs resulted in abnormal cell morphology, up to approximately 75% decrease in mitochondrial activity, loss of lysosomal pH and cell death. OHMs contained significantly less melanin than YHMs, supporting the hypothesis that melanin undergoes degradation during RPE aging. Our results demonstrate that aged MS can be phototoxic to human RPE cells and support a contributing role of MS in RPE aging and in the pathogenesis of age-related macular degeneration.
authors: School of Optometry and Vision Sciences, Cardiff University, Cardiff, UK.
authors: Bartosz
authors: B
authors: Rózanowski
authors: Joyceline
authors: J
authors: Cuenco
authors: Sallyanne
authors: S
authors: Davies
authors: Farukh A
authors: FA
authors: Shamsi
authors: Andrzej
authors: A
authors: Zadło
authors: Pierrette
authors: P
authors: Dayhaw-Barker
authors: Małgorzata
authors: M
authors: Rózanowska
authors: Tadeusz
authors: T
authors: Sarna
authors: Michael E
authors: ME
authors: Boulton
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D008247:Lysosomes / Q000378:metabolism; D020460:Melanosomes / Q000378:metabolism / Q000528:radiation effects*; D008875:Middle Aged; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*
chemical_list: D005609:Free Radicals