[Non-specific adherence of Enterococcus faecalis strains isolated from healthy voluntaries and infection of urinary tract].
Enterococcus faecalis is a component of human and animal gastrointestinal microflora. However, the adhesion is considered to be the key step in the pathogenesis of enterococcal infections and the first step of biofilm formation. We aimed to compare and evaluate adherence of strains considered to be commensal flora (isolated from healthy volunteers) and strains isolated as a pathogen from medical samples in Gdańsk Region The additional aim of this study was to analyze influence of subinhibitory concentration of gentamycin and cAD1 pheromone. Comparison involved 20 strains isolated from healthy voluntaries, 23 strains isolated as etiological agent of urinary tract infection and 16 HLAR strains from other infections. Adherence ability was tasted by turbidymetric method at 550 nm, as o reduction of bacterial inoculum after incubation with hydroksyapatite. Results showed significant difference between commensal and virulent strains as well as between none-inducted and inducted by pheromone. In contrast there was no difference between inducted and non-inducted virulent strains as well as between inducted virulent and inducted not virulent strains. The result shows ability to differentiate this group by non-specific adherence.
Jarzembowski
Tomasz
T
Zakład Mikrobiologii Lekarskiej Katedra Mikrobiologii Akademii Medycznej w Gdańsku.
Dabrowska-Szponar
Maria
M
D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D001426:Bacterial Proteins / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry; D013293:Enterococcus faecalis / Q000187:drug effects / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D005243:Feces / Q000382:microbiology*; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D009842:Oligopeptides / Q000494:pharmacology; D013045:Species Specificity; D014552:Urinary Tract Infections / Q000382:microbiology*; D037521:Virulence Factors / Q000032:analysis / Q000502:physiology
D001426:Bacterial Proteins; D005839:Gentamicins; D009842:Oligopeptides; D037521:Virulence Factors; D017886:Durapatite; C043691:cAD1 bacterial sex hormone