Silica nanoparticle assisted DNA assays for optical signal amplification of conjugated polymer based fluorescent sensors.
A cationic conjugated polymer provided over 110-fold signal amplification for silica nanoparticle based assays, which allow detection of target DNAs at a concentration of 10 pM with a standard fluorometer.
Wang
Yusong
Y
Department of Chemical and Biomolecular Engineering, 4 Engineering Drive 4, National University of Singapore, Singapore.
Liu
Bin
B
D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D055095:Optics and Photonics; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
D005456:Fluorescent Dyes; D011108:Polymers; D012822:Silicon Dioxide; D004247:DNA