Dendritic cells and toll-like receptors 2 and 4 in the ileum of Crohn's disease patients.
We investigated myeloid-dendritic cell (DC) marker and Toll-like receptor (TLR)-2 and 4 distributions in ileal samples from Crohn's disease (CD) patients (n = 14) and controls (n = 13). In controls, no TLR-2+ cells were observed, and higher numbers of TLR-4+ and DC-SIGN+ cells (P < 0.01) were detected in ileal samples when compared versus colonic tissues. In non-inflamed CD ileum, TLR-4+ and DC-SGN+ cells were depleted from superficial areas of the villus, and a significant CD1a+ cell infiltration (P < 0.01) was observed when compared to ileal controls and non-inflamed colonic CD samples. In inflamed CD ileum, DC-SIGN+, CD1a+, TLR-4+and few TLR-4+DC-SIGN+ cells were detected as well as CD83 depletion. No correlation between TLR-2 and DC markers was detected in CD samples. A unique distribution of myeloid-DC markers characterized the CD ileum. Also, the presence of significant amounts of ileal CD1a+ cells may provide a relevant DC-mediated mechanism for antigen recognition in the pathogenesis of CD.
Silva
Manuel A
MA
Intestinal Disease Research Programme, Department of Pathology and Molecular Medicine, McMaster University, Health Science Centre 3N5C, 1200 Main St. West, Hamilton, ON, Canada, L8N 3Z5. msilvamu@hotmail.com
Quera
Rodrigo
R
Valenzuela
Jorge
J
Salim
Sa'ad Y
SY
Söderholm
Johan D
JD
Perdue
Mary H
MH
D000328:Adult; D000704:Analysis of Variance; D003424:Crohn Disease / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D007082:Ileum / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008297:Male; D051195:Toll-Like Receptor 2 / Q000276:immunology*; D051197:Toll-Like Receptor 4 / Q000276:immunology*
D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4