Production of anti-epithelial antibodies and acantholysis by vaccination with an anti-idiotypic antibody, mimicking desmoglein 1.
Theoretically, the immunization of experimental animals with an anti-idiotype antibody may elicit antibodies that recognize epitopes like the original idiotype; this is archived via internal images. Using this strategy, we attempted to produce anti-epithelial antibodies in Balb/c mice immunized with a pemphigus anti-idiotypic determinant. First, when an anti-idiotype antibody was produced in rabbits by immunization with pemphigus immunoglobulin G (IgG), the anti-idiotypic activity was tested successfully. The anti-idiotype IgG was digested with pepsin and purified by gel filtration chromatography to obtain F(ab')(2) fragments, which were used to immunize Balb/c mice. A control group was immunized with normal IgG. The experimental animals immunized with anti-idiotype F(ab')(2) fragments developed anti-epithelial antibodies in the following two months. The elicited antibodies had anti-desmoglein 1 specificity. Additionally, the skin biopsies of these animals exhibited antibody deposition along intercellular spaces of epidermis, and 25% of them developed blisters. Sera and skin biopsies of control Balb/c mice group were negative. In conclusion, the immunization with pemphigus anti-idiotype antibody may elicit anti-epithelial antibodies via internal images. This experimental approach can be used to understand the pathogenic mechanisms of pemphigus.
Avalos-Díaz
Esperanza
E
Department of Immunology, Centro de Biología Experimental, Universidad Autónoma de Zacatecas, México.
López-Robles
Eréndira
E
Alvarado-Flores
Ernesto
E
Herrera-Esparza
Rafael
R
D000051:Acantholysis / Q000276:immunology* / Q000473:pathology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology / Q000378:metabolism*; D000918:Antibody Specificity / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology / Q000378:metabolism; D051183:Desmoglein 1 / Q000276:immunology*; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000187:drug effects* / Q000276:immunology* / Q000473:pathology; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D014611:Vaccination / Q000379:methods; D014612:Vaccines / Q000276:immunology / Q000494:pharmacology*
D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies; D051183:Desmoglein 1; D007140:Immunoglobulin Fab Fragments; D014612:Vaccines