The study of retinal ganglion cell apoptosis induced by different intensities of microwave irradiation.
OBJECTIVE
To observe the influence on the retinal ganglion cells induced by 2,450-MHz microwave. The radiant intensities were 10, 30 and 60 mW x cm(-2).


METHODS
Retinal ganglion cells were cultured in vitro and divided into 4 groups, of which 3 were exposed to different intensities of microwaves and 1 was the control group. The morphological variation of cells was observed by invert microscope. The survival rate was assessed by trypan blue. Annexin V-PI 2-color flow cytometry was used to detect the percentage of earlier apoptosis cells after radiation.


RESULTS
The changes in the morphology of all cells were observed after radiation. The cell survival rates were reduced and the earlier apoptosis increased with growing microwave intensity.


CONCLUSIONS
The 2,450-MHz microwave will cause damage to retinal ganglion cells in a dose-dependent manner.
Zhou
Xin-Rong
XR
Department of Ophthalmology, Harbin Medical University, Second Affiliated Hospital, Harbin, China.
Yuan
Hui-Ping
HP
Qu
Wei
W
Ma
Chun-Yang
CY
Li
Hong-Yi
HY
Wang
Ya
Y
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D066298:In Vitro Techniques; D051379:Mice; D008853:Microscopy; D008872:Microwaves / Q000009:adverse effects*; D011833:Radiation Injuries, Experimental / Q000473:pathology*; D012165:Retinal Ganglion Cells / Q000473:pathology / Q000528:radiation effects*