The histone deacetylase inhibitor ITF2357 selectively targets cells bearing mutated JAK2(V617F).
We investigated the activity of ITF2357, a novel histone deacetylase inhibitor (HDACi) with antitumor activity, on cells carrying the JAK2(V617F) mutation obtained from polycythemia vera (PV) and essential thrombocythemia (ET) patients as well as the HEL cell line. The clonogenic activity of JAK2(V617F) mutated cells was inhibited by low concentrations of ITF2357 (IC(50) 0.001-0.01 microM), 100- to 250-fold lower than required to inhibit growth of normal or tumor cells lacking this mutation. Under these conditions, ITF2357 allowed a seven fold increase in the outgrowth of unmutated over mutated colonies. By western blotting we showed that in HEL cells, ITF2357 led to the disappearance of total and phosphorylated JAK2(V617F) as well as pSTAT5 and pSTAT3, but it did not affect the wild-type JAK2 or STAT proteins in the control K562 cell line. By real-time PCR, we showed that, upon exposure to ITF2357, JAK2(V617F) mRNA was not modified in granulocytes from PV patients while the expression of the PRV-1 gene, a known target of JAK2, was rapidly downmodulated. Altogether, the data presented suggest that ITF2357 inhibits proliferation of cells bearing the JAK2(V617F) mutation through a specific downmodulation of the JAK2(V617F) protein and inhibition of its downstream signaling.
Guerini
V
V
Hematology Unit, Ospedali Riuniti, Bergamo, Italy.
Barbui
V
V
Spinelli
O
O
Salvi
A
A
Dellacasa
C
C
Carobbio
A
A
Introna
M
M
Barbui
T
T
Golay
J
J
Rambaldi
A
A
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D016503:Drug Delivery Systems; D056572:Histone Deacetylase Inhibitors; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D053614:Janus Kinase 2 / Q000032:analysis / Q000235:genetics*; D020125:Mutation, Missense; D011087:Polycythemia Vera / Q000188:drug therapy* / Q000473:pathology; D050791:STAT Transcription Factors / Q000032:analysis; D013920:Thrombocythemia, Essential / Q000188:drug therapy* / Q000473:pathology; D014407:Tumor Cells, Cultured
D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D050791:STAT Transcription Factors; D053614:Janus Kinase 2; C502418:givinostat hydrochloride