title: Application of fluorescence in situ hybridization technique to detect simazine-degrading bacteria in soil samples.
abstract: We propose a new approach to evaluate the natural attenuation capacity of soil by using fluorescence in situ hybridization (FISH). A specific oligonucleotide probe AtzB1 was designed based on the sequence data of the atzB gene involved in the hydrolytic deamination of s-triazines; this gene, located in a multiple copy plasmid was detected by the optimized FISH protocol. Two agricultural soils (Lodi and Henares) with a history of simazine treatments, and two natural soils (Soto and Monza), without previous exposure to simazine, were studied. AtzB1 probe-target cells were found only in the agricultural soils and, in a greater percentage, in the Lodi soil, compared to the Henares one. Moreover, the greatest percentage of AtzB1 probe-target cells in Lodi was accompanied by a greater mineralization rate, compared to the Henares soil. The FISH method used in this study was suitable for the detection of simazine-degrading bacteria and could be a useful indicator of the potential of soil bioremediation.
authors: Complutense University, Avenida Puerta de Hierro s/n, 28040 Madrid, Spain. margamar@vet.ucm.es
authors: Margarita
authors: M
authors: Martín
authors: Alicia
authors: A
authors: Gibello
authors: Carmen
authors: C
authors: Lobo
authors: Mar
authors: M
authors: Nande
authors: Carlos
authors: C
authors: Garbi
authors: Carmen
authors: C
authors: Fajardo
authors: Anna
authors: A
authors: Barra-Caracciolo
authors: Paola
authors: P
authors: Grenni
authors: M José
authors: MJ
authors: Martínez-Iñigo
mesh_terms: D000383:Agriculture; D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D015342:DNA Probes; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction; D012839:Simazine / Q000378:metabolism*; D012988:Soil Microbiology
chemical_list: D015342:DNA Probes; D012839:Simazine