Influence of nutrients on enhancing laccase production by Botryosphaeria rhodina MAMB-05.
The physiological requirements needed to enhance the production of laccases by the ascomycete Botryosphaeria rhodina MAMB-05 in submerged cultivation were examined under non-induced and induced (veratryl alcohol, VA) conditions. Under non-induced conditions (-VA), the initial pH, C:N ratio, and inorganic N source did not influence laccase production, in contrast to Tween 80, soybean oil, and copper, which significantly increased laccase production, and proline and urea, which suppressed laccase formation. In addition, Tween 60 could serve as the sole carbon source for the production of these enzymes. Under VA-induced conditions of fungal growth, factors such as inoculum type, time-point of addition of inducer, initial pH, C:N ratio, and type of N source, influenced the production of laccases; however, unlike the non-induced conditions, proline and urea did not act as suppressors. Each of these physiological conditions exerted different effects on biomass production. The nutritional conditions examined for B. rhodina MAMB-05 are discussed in relation to their influence on fungal growth and laccase production.
Dekker
Robert F H
RF
Department of Biochemistry and Biotechnology-CCE, State University of Londrina, Londrina-PR, CEP 86051-990, Brazil. Robert.Dekker@uclm.es
Barbosa
Aneli M
AM
Giese
Ellen C
EC
Godoy
Saulo D S
SD
Covizzi
Luiz G
LG
D001203:Ascomycota / Q000201:enzymology* / Q000254:growth & development* / Q000378:metabolism; D001592:Benzyl Alcohols / Q000494:pharmacology; D018533:Biomass; D001709:Biotechnology; D002244:Carbon / Q000032:analysis; D003300:Copper / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry*; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D042845:Laccase / Q000096:biosynthesis* / Q000378:metabolism; D009584:Nitrogen / Q000032:analysis; D011136:Polysorbates / Q000494:pharmacology; D013024:Soybean Oil / Q000494:pharmacology
D001592:Benzyl Alcohols; D003470:Culture Media; D011136:Polysorbates; D002244:Carbon; D003300:Copper; D013024:Soybean Oil; D042845:Laccase; C042197:veratryl alcohol; D009584:Nitrogen