Cryopreservation of shoot tips and meristems.
Shoot-tip meristem cryopreservation methodologies are reported for the complementary cryoprotective strategies of vitrification and equilibrium freezing using traditional controlled-rate freezing and chemical additive cryoprotection. Pregrowth, pretreatment, and cold acclimation approaches for the improvement of tolerance to liquid nitrogen are also presented. The chapter concludes by reporting an analytical protocol that profiles volatile hydrocarbon stress markers (for ethylene, hydroxyl radicals, and lipid peroxidation products) during cryopreservation. This method uses noninvasive headspace sampling and gas chromatography, and it is widely applicable across cryogenic systems.
Benson
Erica E
EE
DAMAR, Research Scientists, Conservation, Environmental Science and Biotechnology, Cupar, Fife, Scotland, UK.
Harding
Keith
K
Johnston
Jason W
JW
D000064:Acclimatization / Q000502:physiology; D002849:Chromatography, Gas; D003080:Cold Temperature; D015925:Cryopreservation; D005030:Ethylenes / Q000032:analysis / Q000378:metabolism; D017665:Hydroxyl Radical / Q000032:analysis / Q000378:metabolism; D015227:Lipid Peroxidation; D018519:Meristem; D018520:Plant Shoots
D005030:Ethylenes; D017665:Hydroxyl Radical; C036216:ethylene