[Laboratory diagnosis and molecular characterization of highly pathogenic avian influenza virus (H5N1) in human in Hunan Province in 2005-2006].
To determine the etiologic agents of two atypical pneumonia human cases in Hunan Province in 2005-2006 and to study their pathogenic potential, the patients' respiratory tract samples and sera were collected. The respiratory tract samples were tested by real-time RT-PCR and RT-PCR methods, and the sera by hemagglutination-inhibition assay. Virus was isolated from case 2 and its genome was sequenced and analyzed. Results showed the H5 nucleic acid tests of two cases were positive. The H5-specific antibody titer of the convalescence serum of case 1 showed a 4-fold greater rise than that of the acute phase. And case 2's antibody titer of acute phase was negative. The two atypical pneumonia cases were confirmed as the avian influenza A (H5N1) infection cases. Viral strain A/Hunan/1/2006 was isolated from case 2. Phylogenetic and molecular analysis suggested that 8 gene segments of A/Hunan/1/2006 originated from avian viruses. And A/Hunan/1/2006 was similar with viruses isolated from avian in Hunan Province. The isolated virus did not recombine with human influenza viruses and no obvious variation was observed.
Huang
Yi-Wei
YW
Hunan Provincial Center for Disease Control and Prevention, Changsha 410005, China.
Li
Zi
Z
Zhang
Hong
H
Liu
Yun-Zhi
YZ
Wen
Le-Ying
LY
Li
Jun-Hua
JH
Gao
Li-Dong
LD
Zhao
Xin-Sheng
XS
Li
Zhong-Jie
ZJ
Chen
Bi-Yun
BY
Lan
Yu
Y
Zhou
Lei
L
Shu
Yue-Long
YL
D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D002648:Child; D002681:China; D005260:Female; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis / Q000821:virology*; D008297:Male; D010802:Phylogeny
D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus