Creating a ribonuclease T-tat that preferentially recognizes and hydrolyzes HIV-1 TAR RNA in vitro and in vivo.
A ribonuclease, RNase T-tat, specifically designed to hydrolyze the TAR RNA of HIV-1 virus has been engineered. The protein was made by domain swapping the TAT peptide at the loop 3 position of ribonuclease T1. The RNase T-tat maintains a guanine-specific RNA hydrolytic activity, and characteristically displayed a specific affinity for the TAR RNA of HIV-1. In the in vitro and in vivo assays, the RNase T-tat preferentially inhibited the expression of TAR-bearing mRNA through cis-TAR targeting, suggesting that RNase T-tat may be potentially useful for the disruption of the initial stage of the transcription process of HIV-1 virus.
Dow-Tien
Chen
C
Institute of Genome Sciences, National Yang-Ming University, Taipei, Taiwan, ROC.
Yuan-Jhih
Tsai
T
Alan
Lin
L
D019380:Anti-HIV Agents / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D002460:Cell Line; D015696:Gene Products, tat / Q000737:chemistry / Q000235:genetics; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D006868:Hydrolysis; D008958:Models, Molecular; D015202:Protein Engineering; D021381:Protein Transport; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism; D006163:Ribonuclease T1 / Q000737:chemistry / Q000235:genetics; D012260:Ribonucleases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D019380:Anti-HIV Agents; D015696:Gene Products, tat; D012367:RNA, Viral; D012260:Ribonucleases; C526986:ribonuclease T-tat; D006163:Ribonuclease T1