title: Retinoids accelerate B lineage lymphoid differentiation.
abstract: Retinoids are known to have potent effects on hemopoietic stem cell integrity, and our objective was to learn whether they influence cells destined to replenish the immune system. Total CD19+ B lineage cells increased substantially in the marrow and spleens of all-trans retinoic acid (ATRA)-treated C57BL6 mice, while lymphoid progenitors were reduced. All B lymphoid progenitors were targets of ATRA in culture and overall cell yields declined without reductions in proliferation. Remarkably, ATRA shortened the time required for primitive progenitors to generate CD19+ cells. PCR analysis and a panel of retinoid acid receptor (RAR)/retinoid X receptor agonist treatments suggested that RARalpha mediates these responses. The transcription factors EBF1 and Pax-5 were elevated during treatment and ATRA had similar effects on human B cell differentiation. That is, it inhibited the expansion of human progenitor cells and accelerated their differentiation to B lineage cells. There may be previously unsuspected side effects of ATRA therapy, and the new findings suggest retinoids can normally contribute to the lymphopoietic environment in bone marrow.
authors: Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
authors: Xinrong
authors: X
authors: Chen
authors: Brandt L
authors: BL
authors: Esplin
authors: Karla P
authors: KP
authors: Garrett
authors: Robert S
authors: RS
authors: Welner
authors: Carol F
authors: CF
authors: Webb
authors: Paul W
authors: PW
authors: Kincade
mesh_terms: D000818:Animals; D018941:Antigens, CD19 / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D002454:Cell Differentiation; D019070:Cell Lineage; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018655:Lymphocyte Count; D038041:Lymphopoiesis; D051379:Mice; D008810:Mice, Inbred C57BL; D051757:PAX5 Transcription Factor / Q000235:genetics / Q000378:metabolism; D054448:Precursor Cells, B-Lymphoid / Q000187:drug effects*; D047488:Retinoid X Receptors / Q000235:genetics / Q000378:metabolism; D012176:Retinoids / Q000494:pharmacology / Q000502:physiology; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology* / Q000502:physiology
chemical_list: D018941:Antigens, CD19; D004268:DNA-Binding Proteins; C489921:Ebf1 protein, mouse; D051757:PAX5 Transcription Factor; C497282:Pax5 protein, mouse; D047488:Retinoid X Receptors; D012176:Retinoids; D015534:Trans-Activators; D014212:Tretinoin