SPG/IND-induced septic shock in a LPS-low responder strain, C3H/HeJ mice.
The administration of beta-glucan (sonifilan; SPG) in combination with a non-steroidal anti-inflammatory drug, indomethacin (IND), induced lethal septic shock in mice. To demonstrate the influence of bacterial lipopolysaccharide (LPS) in this lethal toxicity, LPS non-responder C3H/HeJ mice were used to compare features of sepsis and physicochemical parameters in the present study. The administration of SPG and IND induced the death of C3H/HeJ mice, lowering rectal temperature, reducing body weight, increasing serum tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) levels, shortening the gastrointestinal tract, and increasing the GOT/GPT level. Microbial translocation to various organs was also significantly increased. These results strongly suggested that LPS-non-responding strain also induced septic shock in this experimental model, and other pathogen-associated molecular patterns (PAMPs) may significantly contribute to the septic shock.
Saito
Maki
M
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Nameda
Sachiko
S
Miura
Noriko N
NN
Adachi
Yoshiyuki
Y
Ohno
Naohito
N
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001831:Body Temperature; D001835:Body Weight; D041981:Gastrointestinal Tract / Q000473:pathology; D007213:Indomethacin / Q000633:toxicity*; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D012772:Shock, Septic / Q000139:chemically induced*; D012566:Sizofiran / Q000633:toxicity*; D016019:Survival Analysis; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D012566:Sizofiran; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D007213:Indomethacin