Bioproduction of D-psicose from allitol with Enterobacter aerogenes IK7: a new frontier in rare ketose production.
D-psicose, a new alternative sweetener, was produced from allitol by microbial oxidation of the newly isolated strain Enterobacter aerogenes IK7. Cells grown in tryptic soy broth medium (TSB) supplemented with D-mannitol at 37 degrees C were found to have the best oxidation potential. The cells, owing to broad substrate specificity, oxidized various polyols (tetritol, pentitol, and hexitol) to corresponding rare ketoses. By a resting cell reaction, 10% of allitol was completely transformed to the product D-psicose, which thus becomes economically feasible for the mass production of D-psicose. Finally, the product was crystallized and confirmed to be D-psicose by analytical methods.
Gullapalli
Pushpakiran
P
Rare Sugar Research Center and Faculty of Agriculture, Kagawa Univeristy, Kita-gun, Kagawa, Japan.
Takata
Goro
G
Poonperm
Wayoon
W
Rao
Devendar
D
Morimoto
Kenji
K
Akimitsu
Kazuya
K
Tajima
Shigeyuki
S
Izumori
Ken
K
D021902:Enterobacter aerogenes / Q000378:metabolism*; D005632:Fructose / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D013237:Stereoisomerism; D013402:Sugar Alcohols / Q000378:metabolism*; D013549:Sweetening Agents / Q000737:chemistry / Q000378:metabolism*
D013402:Sugar Alcohols; D013549:Sweetening Agents; C003243:psicose; D005632:Fructose; C527823:allitol