title: Yeast ribosomal stalk heterogeneity in vivo shown by two-photon FCS and molecular brightness analysis.
abstract: The stalk of Saccharomyces cerevisiae ribosomes contains, on average, five distinct proteins, namely P0 and four acidic proteins, P1alpha, P1beta, P2alpha, and P2beta. Each ribosome contains only one copy of P0, but the distribution of the acidic proteins among the ribosome population in vivo has not been determined. Using two-photon fluorescence correlation spectroscopy and scanning FCS, on cells expressing EGFP-tagged P0, P1, and P2 proteins, we show, with brightness analysis, that individual yeast ribosomes in vivo are compositionally heterogeneous in regard to P1alpha, P1beta, P2alpha, and P2beta. These results are relevant to the hypothesis, based on in vitro studies, that the overall cellular pattern of expressed proteins can be determined by the distribution of the stalk proteins among the ribosome population.
authors: Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, 28049 Madrid, Spain.
authors: Alberto
authors: A
authors: García-Marcos
authors: Susana A
authors: SA
authors: Sánchez
authors: Pilar
authors: P
authors: Parada
authors: John
authors: J
authors: Eid
authors: David M
authors: DM
authors: Jameson
authors: Miguel
authors: M
authors: Remacha
authors: Enrico
authors: E
authors: Gratton
authors: Juan P G
authors: JP
authors: Ballesta
mesh_terms: D008856:Microscopy, Fluorescence / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D012270:Ribosomes / Q000737:chemistry* / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*