Triplex-forming ability of modified oligonucleotides.
We present our studies on the ability of several different nucleotide analogs as triplex-forming oligonucleotides. The modifications tested include 4'-C-hydroxymethyl, LNA, 2'-amino-LNA and N2'-functionalized 2'-amino-LNA. Triplexes containing monomers of N2'-glycyl-functionalized 2'-amino-LNA are particularly stable.
Højland
Torben
T
Nucleic Acid Center, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark. tohoe00@student.sdu.dk
Babu
B Ravindra
BR
Bryld
Torsten
T
Wengel
Jesper
J
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D015497:HIV-1 / Q000235:genetics; D009711:Nucleotides / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics; D012266:Ribose / Q000031:analogs & derivatives* / Q000737:chemistry; D013938:Thymidine Monophosphate / Q000031:analogs & derivatives* / Q000737:chemistry
C434798:2'-O-4'-C-methylene-ribofuranosyl nucleotide; C520121:7-(2-cyanoethoxy(diisopropylamino)phosphinoxy)-1-(4,4'-dimethoxytrityloxymethyl)-5-(9'-fluorenylmethoxycarbonyl)-3-(thymin-1-yl)-2-oxa-5-azabicyclo(2.2.1)heptane; D009711:Nucleotides; D009841:Oligonucleotides; C051802:triplex DNA; D013938:Thymidine Monophosphate; D012266:Ribose; D004247:DNA