title: Production of chitinolytic enzymes by a strain (BM17) of Paenibacillus pabuli isolated from crab shells samples collected in the east sector of central Tyrrhenian Sea.
abstract: Nineteen bacterial isolates were grown in shaken cultures in media containing chitin as carbon source and different additional nitrogen sources such as yeast nitrogen base (YNB), yeast extract (YE), corn steep liquor (CSL) and ammonium sulfate. Strain BM17 showed the highest activity (200 U/l) in medium containing Chitin (1%) and YNB (0.5%). Molecular analysis of the 16S rRNA gene showed that strain BM17 belongs to the species Paenibacillus pabuli (99.72% homology). The enzyme activity started after 12-24 h; exponential enzyme production was recorded from the 24th h and lasted till the 96th h of incubation when activity peaked to decrease thereafter. Medium optimisation was carried out by Response Surface Methodology (RSM) considering the effects of chitin, corn steep liquor and yeast extract. BM17 chitinolytic activity was induced by chitin but the increase of its concentration did not have significant effects on the enzyme activity. By contrast, the nitrogen source, particularly YE, strongly affected the enzyme production.
authors: Istituto del Agua, University of Granada, Granada, Spain.
authors: Belen
authors: B
authors: Juarez-Jimenez
authors: Belen
authors: B
authors: Rodelas
authors: M Victoria
authors: MV
authors: Martinez-Toledo
authors: Jesus
authors: J
authors: Gonzalez-Lopez
authors: Silvia
authors: S
authors: Crognale
authors: Anna M
authors: AM
authors: Gallo
authors: Chiara
authors: C
authors: Pesciaroli
authors: Massimiliano
authors: M
authors: Fenice
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D003386:Brachyura / Q000033:anatomy & histology* / Q000382:microbiology*; D002686:Chitin / Q000378:metabolism*; D008522:Mediterranean Sea; D050296:Microbial Viability; D010802:Phylogeny; D013499:Surface Properties
chemical_list: D002686:Chitin