Early effects triggered by Larrea divaricata Cav. on murine macrophages at apoptotic concentrations.
Decoction and infusion of Larrea divaricata were tested at apoptotic concentrations (1 and 4 mg/ml) on peritoneal murine macrophages. Consistent changes were observed after incubation with 4 mg/ml decoction. Phagocytosis of zymosan, lysosomal enzyme activity, nitric oxide production, TNF-alpha release, and expression of CD14, TLR4, and CR3 increased significantly. Decoction at 1 and 4 mg/ml increased the binding of LPS-FITC. Apoptosis triggered by L. divaricata decoction is consequence of cell activation. The effects are independent of nordihydroguaiaretic acid. This "activation and death" could be the mechanism of L. divaricata to exert the antituberculosis effect known in folk medicine.
Davicino
Roberto
R
Departamento de Bioquímica y Ciencias Biológicas, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis. Chacabuco y Pedernera, San Luis, Argentina.
Mattar
Aida
A
Casali
Yolanda
Y
Porporatto
Carina
C
Correa
Silvia G
SG
Micalizzi
Blas
B
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D005260:Female; D005434:Flow Cytometry; D066298:In Vitro Techniques; D032442:Larrea / Q000737:chemistry*; D008033:Limulus Test; D018950:Lipopolysaccharide Receptors / Q000187:drug effects; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000633:toxicity; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D009637:Masoprocol / Q000494:pharmacology; D051379:Mice; D009569:Nitric Oxide / Q000032:analysis / Q000096:biosynthesis; D010587:Phagocytosis / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D016897:Respiratory Burst / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000096:biosynthesis
D000975:Antioxidants; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D009637:Masoprocol; D000135:Acid Phosphatase