title: Entamoeba histolytica: cyst-like structures in vitro induction.
abstract: The cyst of Entamoeba histolytica is responsible for amebiasis infection. However, no axenic in vitro system exists that promotes mass encystation for studying this process of this human-infecting parasite. Cyst-like structures of E. histolytica obtained in this work were induced using TYI-S-33 media in combination with enterobacterias Escherichia coli and Enterococcus faecalis conditioned media, high CO2 tension and histamine. Cyst-like structures showed the same characteristics of a typical E. histolytica cyst: aggregation, resistance to 0.15% sarcosyl for 10 min, high signal of fluorescence under UV light when stained with 10% calcofluor M2r and the surface topology showed a wrinkled wall. In addition these structures are multinucleated with condensed chromatin attached to nuclear membrane, contain big vacuoles and ribonucleoproteic helices in the cytoplasm and also present a thin cell wall. Last all characteristics are all the same as a typical of E. histolytica cyst.
authors: Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Av. Pedro de Alba y Manuel L. Barragán S/N Ciudad Universitaria, C.P. 66451, A.P. 67-F, San Nicolás de los Garza, Nuevo León, Mexico.
authors: M P
authors: MP
authors: Barrón-González
authors: L
authors: L
authors: Villarreal-Treviño
authors: D
authors: D
authors: Reséndez-Pérez
authors: B D
authors: BD
authors: Mata-Cárdenas
authors: M R
authors: MR
authors: Morales-Vallarta
mesh_terms: D000818:Animals; D003470:Culture Media; D004748:Entamoeba histolytica / Q000502:physiology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence
chemical_list: D003470:Culture Media