title: The Suv39h-HP1 histone methylation pathway is dispensable for enrichment and protection of cohesin at centromeres in mammalian cells.
abstract: Sister chromatids are physically connected by cohesin complexes. This sister chromatid cohesion is essential for the biorientation of chromosomes on the mitotic and meiotic spindle. In many species, cohesion between chromosome arms is partly dissolved in prophase of mitosis, whereas cohesion is protected at centromeres until the onset of anaphase. In vertebrates, the protein Sgo1, protein phosphatase 2A, and several other proteins are required for protection of centromeric cohesin in early mitosis. In fission yeast, the recruitment of heterochromatin protein Swi6/HP1 to centromeres by the histone-methyltransferase Clr4/Suv39h is required for enrichment of cohesin at centromeres already in interphase. We have tested if the Suv39h-HP1 histone methylation pathway is also required for enrichment and mitotic protection of cohesin at centromeres in mammalian cells. We show that cohesin and HP1 proteins partially colocalize at mitotic centromeres but that cohesin localization is not detectably altered in mouse embryonic fibroblasts that lack Suv39h genes and in which HP1 proteins can, therefore, not be properly enriched in pericentric heterochromatin. Our data indicate that the Suv39h-HP1 pathway is not essential for enrichment and mitotic protection of cohesin at centromeres in mammalian cells.
authors: Research Institute of Molecular Pathology (IMP), Dr. Bohr-Gasse 7, 1030 Vienna, Austria.
authors: Birgit
authors: B
authors: Koch
authors: Stephanie
authors: S
authors: Kueng
authors: Christine
authors: C
authors: Ruckenbauer
authors: Kerstin S
authors: KS
authors: Wendt
authors: Jan-Michael
authors: JM
authors: Peters
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002503:Centromere / Q000648:ultrastructure; D047369:Chromatin Immunoprecipitation; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D008540:Meiosis; D008745:Methylation; D008780:Methyltransferases / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008856:Microscopy, Fluorescence; D008938:Mitosis; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D012568:Schizosaccharomyces; D008941:Spindle Apparatus; D000097722:Cohesins
chemical_list: D018797:Cell Cycle Proteins; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D008780:Methyltransferases; D012097:Repressor Proteins; D000097722:Cohesins; C498700:Suv39h1 protein, mouse