TGF-beta1 and high levels of glucose do not increase insulin cell proportions in the avian embryonic pancreas.
TGF-beta1 is thought to decrease the proportion of embryonic pancreatic beta-cells with respect to alpha-cells, whereas glucose is thought to enhance beta-cell proportions in rats. However, chick pancreatic cells may respond in a dissimilar way to glucose. Thus, the effect of TGF-beta1 on the proportion of beta-cells in embryonic chick dorsal pancreatic buds (DPBs) in vitro was tested with short-term exposure to high levels of glucose. Five-day-old chick DPBs were cultured on growth factor-reduced Matrigel, which contains reduced levels of growth factors including TGF-beta1, and a variety of culture media with and without high levels of glucose. TGF-beta1 reduced the proportion of beta-cells, as expected. A similar decrease in the proportion of beta-cells occurred in the presence of high levels of glucose.
Marais
A
A
Embryonic Differentiation and Development Research Programme, School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, Johannesburg, South Africa. anita.marais@gmail.com
Goven-Shiba
P T
PT
Kramer
B
B
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000378:metabolism; D010179:Pancreas / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D051381:Rats; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
D007328:Insulin; D053773:Transforming Growth Factor beta1; D005947:Glucose