A faster and more accurate assay for intracellular replication of Legionella pneumophila in amoebae hosts.
A faster, more sensitive, and more accurate method to study intracellular replication of Legionella pneumophila in amoeboid hosts is described. This assay relies on an automated plate-reader to examine the intracellular growth and release of bacteria in real-time. Our experiments using this method have already revealed new insights into the kinetics of intracellular replication of L. pneumophila in Acanthamoeba castellanii.
Coil
David A
DA
Laboratory of Bacteriology, Rega Institute for Medical Research, Katholieke Universiteit, Leuven, Minderbroedersstraat 10, Leuven, Belgium.
Anné
Jozef
J
Lammertyn
Elke
E
D016841:Amoebida / Q000382:microbiology*; D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D016952:Legionella pneumophila / Q000235:genetics / Q000502:physiology*; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods