[Apoptosis-related gene expression of human villous trophoblasts exposed to 50 Hz magnetic field].
OBJECTIVE
To study apoptosis-related gene expression of human villous trophoblasts exposed to 50 Hz magnetic field and to investigate the possible mechanism of human reproductive health effects caused by 50 Hz magnetic field.


METHODS
Cultured human villous trophoblasts were exposed to 50 Hz magnetic field at 0.4 mT for 6, 48, 72 hours. Gene expressions of Bcl-2, Bax, Caspase-3, p53 and Fas were analyzed using real-time reverse transcription polymerase chain reaction (RT-PCR) assay.


RESULTS
Within 72 hours, the average fold change for each gene was near 1.00, and there was no significant difference on expression pattern in each gene between exposure and control groups (P > 0.05).


CONCLUSIONS
0.4 mT 50 Hz magnetic field does not affect the apoptosis-related gene expression of human villous trophoblasts in vitro.
Tan
Qiu
Q
Bioelectromagnetics Laboratory, Zhejiang University, Hangzhou 310058, China.
Sun
Wen-jun
WJ
Pan
Yong-miao
YM
Sun
Hui-lan
HL
Hu
Gen-lin
GL
Jiang
Huai
H
D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D060526:Magnetic Fields / Q000009:adverse effects*; D011247:Pregnancy; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D014327:Trophoblasts / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism
C494826:BAX protein, human; C491901:FAS protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D019014:fas Receptor; C505407:CASP3 protein, human; D053148:Caspase 3