Lipopolysaccharide induces adipose differentiation-related protein expression and lipid accumulation in the liver through inhibition of fatty acid oxidation in mice.
BACKGROUND
In the present study, we examined the effect of lipopolysaccharide (LPS) on liver histopathology with special reference to lipid metabolism in mice.


METHODS
Mice were injected with LPS intraperitoneally, and its effect on the liver was investigated pathologically and biochemically.


RESULTS
Oil-red O staining and adipose differentiation-related protein (ADRP) immunohistochemistry demonstrated that injection of LPS transiently induced lipid accumulation and ADRP expression in hepatocytes, especially around the portal vein. Microscopic observation revealed that lipid accumulation started 12 h after LPS injection. Time-course studies showed that LPS rapidly, within 2 h, decreased hepatic expression of nuclear hormone receptors, including peroxisome proliferator-activated receptor (PPAR) alpha. LPS inhibited the expression of PPARalpha-target genes involved in fatty acid oxidation in the liver such as those coding for enoyl-CoA hydratase, acyl-CoA dehydrogenase, and carnitine palmitoyl transferase-1, whereas LPS also suppressed the expression of genes related to fatty acid synthesis such as those for fatty acid synthase, stearoyl-CoA desaturase, and acetyl-CoA carboxylase alpha.


CONCLUSIONS
LPS induces transient lipid accumulation and expression of ADRP in the liver through inhibition of fatty acid oxidation by downregulation of the PPARalpha-related transcriptional mechanism.
Ohhira
Masumi
M
Department of General Medicine, Asahikawa Medical College, 2-1 Midorigaoka-higashi, Asahikawa 078-8510, Japan.
Motomura
Wataru
W
Fukuda
Mitsuko
M
Yoshizaki
Takayuki
T
Takahashi
Nobuhiko
N
Tanno
Satoshi
S
Wakamiya
Nobutaka
N
Kohgo
Yutaka
Y
Kumei
Shima
S
Okumura
Toshikatsu
T
D000818:Animals; D050356:Lipid Metabolism; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000473:pathology*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D010084:Oxidation-Reduction; D047493:PPAR alpha / Q000096:biosynthesis; D000070780:Perilipin-2; D015854:Up-Regulation
D008070:Lipopolysaccharides; D008565:Membrane Proteins; D047493:PPAR alpha; D000070780:Perilipin-2; C000602983:Plin2 protein, mouse