title: Induction of carbonic anhydrase IX by hypoxia and chemical disruption of oxygen sensing in rat fibroblasts and cardiomyocytes.
abstract: CA IX is an active transmembrane carbonic anhydrase isoform functionally implicated in cell adhesion and pH control. Human CA IX is strongly induced by hypoxia and frequently associated with various tumors. In this study, we investigated the expression of the rat CA IX in response to chronic hypoxia and to treatment with chemical compounds that disrupt oxygen sensing, including dimethyloxalylglycine, dimethylester succinate, diazoxide, and tempol. We brought the evidence that expression of CA IX is regulated by hypoxia and hypoxia-mimicking compounds in immortalized Rat2 fibroblasts and BP6 rat fibrosarcoma cells in a cell-type-specific manner. We also demonstrated, for the first time, that CA IX is expressed in hypoxic primary rat cardiomyocytes and in immortalized H9c2 cardiomyocytes exposed to physiological or chemical hypoxia and that CA IX expression is increased in hypoxic rat tissues in vivo. Our findings suggest that CA IX expression is not limited to cancer but may be also induced in other pathological situations associated with ischemia or metabolic disturbances leading to activation of the HIF pathway. These data support the view that rats can represent useful model for studies of CA IX as a component of endogenous protection mechanisms associated with hypoxia or perturbed oxygen sensing.
authors: Institute of Virology, Centre of Excellence in Cardiovascular Research, Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava, Slovak Republic.
authors: Tereza
authors: T
authors: Holotnakova
authors: Attila
authors: A
authors: Ziegelhoffer
authors: Anna
authors: A
authors: Ohradanova
authors: Alzbeta
authors: A
authors: Hulikova
authors: Marie
authors: M
authors: Novakova
authors: Juraj
authors: J
authors: Kopacek
authors: Jaromir
authors: J
authors: Pastorek
authors: Silvia
authors: S
authors: Pastorekova
mesh_terms: D000600:Amino Acids, Dicarboxylic / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases / Q000235:genetics / Q000378:metabolism*; D015687:Cell Hypoxia / Q000502:physiology*; D002460:Cell Line; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003497:Cyclic N-Oxides / Q000494:pharmacology; D003981:Diazoxide / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D008297:Male; D023421:Models, Animal; D008954:Models, Biological; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013113:Spin Labels
chemical_list: D000600:Amino Acids, Dicarboxylic; D000975:Antioxidants; D003497:Cyclic N-Oxides; C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D013113:Spin Labels; C526285:Ca9 protein, rat; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases; D003981:Diazoxide; D010100:Oxygen; C001803:tempol; C040947:oxalylglycine