Biophysical properties of quadruplexes containing two or three 8-bromodeoxyguanosine residues.
A physico-chemical characterization, based on NMR and CD spectroscopy, of quadruplexes formed by the oligonucleotide d(TGGGT), where two or three Gs are substituted by 8-bromo-2'-deoxyguanosine residues (dGBr), is reported. The oligonucleotidic sequences d(TGBr GBr GT), d(TGBr GGBr T), d(TGGBr GBr T), and d(TGBr GBr GBr T) have been synthesized. Only sequences d(TGBr GGBr T) and d(TGBr GBr GT) were able to fold into a well defined quadruplex structure, and their CD profiles and thermal stabilities turned out to be very different from those observed for the natural counterpart, indicating that the 8-Br-dG residues dramatically affect the structure of the quadruplex.
Petraccone
Luigi
L
Dipartimento di Chimica, Università degli Studi di Napoli Federico II, Napoli, Italy.
Duro
Ida
I
Randazzo
Antonio
A
Virno
Ada
A
Mayol
Luciano
L
Giancola
Concetta
C
D002942:Circular Dichroism; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry; D054856:G-Quadruplexes; D009682:Magnetic Resonance Spectroscopy; D009691:Nucleic Acid Denaturation; D011522:Protons; D013816:Thermodynamics
D011522:Protons; D003849:Deoxyguanosine