Association between single-nucleotide polymorphisms in hormone metabolism and DNA repair genes and epithelial ovarian cancer: results from two Australian studies and an additional validation set.
Although some high-risk ovarian cancer genes have been identified, it is likely that common low penetrance alleles exist that confer some increase in ovarian cancer risk. We have genotyped nine putative functional single-nucleotide polymorphisms (SNP) in genes involved in steroid hormone synthesis (SRD5A2, CYP19A1, HSB17B1, and HSD17B4) and DNA repair (XRCC2, XRCC3, BRCA2, and RAD52) using two Australian ovarian cancer case-control studies, comprising a total of 1,466 cases and 1,821 controls of Caucasian origin. Genotype frequencies in cases and controls were compared using logistic regression. The only SNP we found to be associated with ovarian cancer risk in both of these two studies was SRD5A2 V89L (rs523349), which showed a significant trend of increasing risk per rare allele (P = 0.00002). We then genotyped another SNP in this gene (rs632148; r(2) = 0.945 with V89L) in an attempt to validate this finding in an independent set of 1,479 cases and 2,452 controls from United Kingdom, United States, and Denmark. There was no association between rs632148 and ovarian cancer risk in the validation samples, and overall, there was no significant heterogeneity between the results of the five studies. Further analyses of SNPs in this gene are therefore warranted to determine whether SRD5A2 plays a role in ovarian cancer predisposition.
Beesley
Jonathan
J
Queensland Institute of Medical Research, Herston, Queensland, Australia.
Jordan
Susan J
SJ
Spurdle
Amanda B
AB
Song
Honglin
H
Ramus
Susan J
SJ
Kjaer
Suzanne Kruger
SK
Hogdall
Estrid
E
DiCioccio
Richard A
RA
McGuire
Valerie
V
Whittemore
Alice S
AS
Gayther
Simon A
SA
Pharoah
Paul D P
PD
Webb
Penelope M
PM
Chenevix-Trench
Georgia
G
D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000235:genetics; D001315:Australia; D016022:Case-Control Studies; D004260:DNA Repair / Q000235:genetics*; D045643:DNA Repair Enzymes / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D012739:Gonadal Steroid Hormones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics* / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results; D012307:Risk Factors
D012739:Gonadal Steroid Hormones; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase; D045643:DNA Repair Enzymes