The ultrastructural research of liver in experimental obstructive jaundice and effect of honey.
BACKGROUND
To examine the effects of honey on oxidative stress and apoptosis in experimental obstructive jaundice model.


METHODS
Thirty rats were divided into 3 groups: group I, sham-operated; group II, ligation and division of the common bile duct (BDL); group III, BDL followed by oral supplementation of honey 10 g/kg/d. Liver samples were examined under light microscope and transmission electron microscope. Hepatocyte apoptosis was quantitated using the terminal deoxy-nucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay. Plasma and blood malondialdehyde (MDA) and glutation activities were measured for determining the oxidative stress.


RESULTS
The liver levels of MDA and GSH were significantly different between the honey and BDL groups (P = .006 and .001, respectively). However, there was no significant difference between the plasma MDA and GSH levels of these groups (P > .05). In group III, significant reductions in the size of enlarged hepatocytes and the edema were demonstrated. The dilatation of the bile canaliculi dramatically turned to original dimention. By TUNEL assay, it was shown that administration of honey decreased the number of apoptotic cells.


CONCLUSIONS
In the present study, we found that honey diminished the negative effects of BDL on the hepatic ultrastructure. We conclude that this effect might be due to its antioxidant and anti-inflammatory activities.
Kilicoglu
Bulent
B
Ankara Training and Research Hospital, 4th General Surgery Department, Ankara, Turkey.
Gencay
Cem
C
Kismet
Kemal
K
Serin Kilicoglu
Sibel
S
Erguder
Imge
I
Erel
Serap
S
Sunay
Asli Elif
AE
Erdemli
Esra
E
Durak
Ilker
I
Akkus
Mehmet Ali
MA
D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D004195:Disease Models, Animal; D022781:Hepatocytes / Q000166:cytology; D006722:Honey; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D041781:Jaundice, Obstructive / Q000473:pathology / Q000503:physiopathology; D008099:Liver / Q000473:pathology / Q000648:ultrastructure*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000502:physiology*; D011336:Probability; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012680:Sensitivity and Specificity
D008315:Malondialdehyde