title: Combinatorial synthesis of peptide arrays onto a microchip.
abstract: Arrays promise to advance biology through parallel screening for binding partners. We show the combinatorial in situ synthesis of 40,000 peptide spots per square centimeter on a microchip. Our variant Merrifield synthesis immobilizes activated amino acids as monomers within particles, which are successively attracted by electric fields generated on each pixel electrode of the chip. With all different amino acids addressed, particles are melted at once to initiate coupling. Repetitive coupling cycles should allow for the translation of whole proteomes into arrays of overlapping peptides that could be used for proteome research and antibody profiling.
authors: German Cancer Research Center, INF 580, 69120 Heidelberg, Germany.
authors: Mario
authors: M
authors: Beyer
authors: Alexander
authors: A
authors: Nesterov
authors: Ines
authors: I
authors: Block
authors: Kai
authors: K
authors: König
authors: Thomas
authors: T
authors: Felgenhauer
authors: Simon
authors: S
authors: Fernandez
authors: Klaus
authors: K
authors: Leibe
authors: Gloria
authors: G
authors: Torralba
authors: Michael
authors: M
authors: Hausmann
authors: Ulrich
authors: U
authors: Trunk
authors: Volker
authors: V
authors: Lindenstruth
authors: F Ralf
authors: FR
authors: Bischoff
authors: Volker
authors: V
authors: Stadler
authors: Frank
authors: F
authors: Breitling
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D020650:Combinatorial Chemistry Techniques; D004560:Electricity; D010455:Peptides / Q000138:chemical synthesis*; D040081:Protein Array Analysis / Q000379:methods
chemical_list: D000596:Amino Acids; D010455:Peptides