Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae.
The conditional ero1-1 mutant, deficient in the ER-localized PDI oxidase Ero1p, is blocked in disulfide bond formation under restrictive conditions, such as high temperature, lack of oxygen, or high concentrations of membrane-permeant thiols. Previous studies of the physiological consequences of the ero1-1 mutation were carried out in a leu2 mutant. The ero1-1 leu2 strain does not grow in standard synthetic complete medium at 30 degrees C, a defect that can be remedied by increasing the L-leucine concentration in the medium or by transforming the ero1-1 leu2 strain with the LEU2 wild-type allele. In addition, the LEU2 gene can partially complement the growth impairment at 37 degrees C of the ero1-1 leu2 mutant. The leucine transporter Bap2p exhibits a dramatic decrease in stability in an ero1-1 strain, which may account for the pronounced leucine demand observed in the ero1-1 leu2 mutant.
López-Mirabal
H Reynaldo
HR
Carlsberg Laboratory, Copenhagen Valby, Denmark.
Winther
Jakob R
JR
Kielland-Brandt
Morten C
MC
D050539:3-Isopropylmalate Dehydrogenase; D026905:Amino Acid Transport Systems / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D009154:Mutation; D050862:Oxidoreductases Acting on Sulfur Group Donors; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
D026905:Amino Acid Transport Systems; C097094:BAP2 protein, S cerevisiae; D004268:DNA-Binding Proteins; D006023:Glycoproteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D050539:3-Isopropylmalate Dehydrogenase; C524993:LEU2 protein, S cerevisiae; D050862:Oxidoreductases Acting on Sulfur Group Donors; C113186:ERO1 protein, S cerevisiae; D007930:Leucine