Solid support post-conjugation of amino acids and a phenanthroline derivative to a central position in peptide nucleic acids.
A solid phase synthesis strategy for post-conjugation of amino acids and a phenanthroline derivative to peptide nucleic acids is described. The peptide nucleic acids, synthesized by 9-fluorenylmethyloxycarbonyl chemistry on TentaGel S Rink Amide resin, have an internally placed unit carrying an amino linker with 4-methyltrityl protection. Methyltrityl removal by mild acidic conditions and conjugation of amino acids or a phenanthroline derivative, via an amide or urea linker, was performed on-resin after completion of the chain assembly. This solid phase methodology resulted in excellent purities of the crude conjugates.
Sandbrink
Jessica
J
Department of Biosciences and Nutrition, Karolinska Institutet, NOVUM, S-14157 Huddinge, Sweden.
Murtola
Merita
M
Strömberg
Roger
R
D000596:Amino Acids / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D010618:Phenanthrolines / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry
D000596:Amino Acids; D020135:Peptide Nucleic Acids; D010618:Phenanthrolines; D011137:Polystyrenes; C101297:Tentagel resin