Role of modulator recognition factor 2 in adipogenesis and leptin expression in 3T3-L1 cells.
The complex network of adipogenic transcription factors regulates adipocyte differentiation, obesity, and insulin resistance. Modulator recognition factor (Mrf) 2 knockout mice exhibit defects in fat accumulation and are protected from diet-induced obesity, suggesting that Mrf2 deficiency affects adipogenesis. Here, we report that the gene expressions of the 2 isoforms of the transcription factors Mrf2, Mrf2alpha, and Mrf2beta, were induced upon adipogenesis in 3T3-L1 cells. Mrf2 mRNA expression was sensitive to stimulation by insulin, dexamethasone, and TNF-alpha in 3T3-L1 preadipocytes and differentiated adipocytes. Down-regulation of Mrf2alpha and Mrf2beta gene expressions induced by small interfering RNAs increased the mRNA expression of leptin. These results indicate that Mrf2 can be a potential regulator of adipocyte differentiation and a potential repressor of leptin.
Dong
Jie
J
Department of Cardiovascular Medicine, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
Ishimori
Naoki
N
Paigen
Beverly
B
Tsutsui
Hiroyuki
H
Fujii
Satoshi
S
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D050156:Adipogenesis / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation; D004268:DNA-Binding Proteins / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D020738:Leptin / Q000378:metabolism*; D051379:Mice; D014157:Transcription Factors / Q000378:metabolism*
C503806:Arid5b protein, mouse; D004268:DNA-Binding Proteins; D020738:Leptin; D014157:Transcription Factors