[Construction of rabbit hemorrhagic disease virus replicons and its replication in RK-13 cells].
To provide an efficient and safe technology platform for studying the replication and pathogenesis mechanisms of RHDV, the interaction between the RHDV and its host cells, a replicon system of RHDV, was constructed based on the infectious cDNA clone of RHDV, in which VP60 gene encoding the capsid protein was deleted, but all the necessary protease coding regions and non-coding regions were retained. Results from RT-PCR, IFA and qRT-PCR confirmed that the replicon RNA could efficiently replicate in RK-13 cells. Besides, the results also suggested that the capsid protein which is the structural protein of RHDV is necessary for maintaining the viral infectivity.
Liu
Guang-Qing
GQ
Institute of Virology & Biotechnology, Zhejiang Academy of Agriculture Science, Hangzhou 310021, Zhejiang, China.
Ni
Zheng
Z
Yun
Tao
T
Yu
Bing
B
Zhu
Jin-Mei
JM
Hua
Jiong-Gang
JG
Chen
Jian-Ping
JP
D000818:Animals; D036022:Capsid Proteins / Q000502:physiology; D005455:Fluorescent Antibody Technique; D017929:Hemorrhagic Disease Virus, Rabbit / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis*; D011817:Rabbits; D012093:Replicon; D020133:Reverse Transcriptase Polymerase Chain Reaction
D036022:Capsid Proteins; D012367:RNA, Viral