In vitro inhibition of sole aquabirnavirus by Senegalese sole Mx.
Senegalese sole, Solea senegalensis, is a flat fish of growing interest in European aquaculture. In its culture viral infections are constant threats, thus understanding antiviral defences is a key factor for a successful industry. Mx proteins are IFN-induced proteins widespread in eukaryotes; however, their antiviral activity is unclear and the results variable among species. Therefore assessment of the putative Mx antiviral activity in each species is of interest. Our group has recently cloned the Senegalese sole Mx (SsMx) cDNA and in this study its antiviral activity was assessed by infecting CHSE-214 cells expressing recombinant SsMx, with sole aquabirnavirus. The antiviral activity against this pathogen was demonstrated by reduction in induced cytopathic effects, reduction in virus yield and decrease in viral transcripts. These findings contribute to our understanding of fish antiviral mechanisms and open the possibility of using this protein as a tool for fighting viral infections in aquaculture.
Fernández-Trujillo
M Alejandra
MA
Department of Genetics, Faculty of Sciences, University of Málaga, Campus de Teatinos, Málaga, Spain.
García-Rosado
Esther
E
Alonso
M Carmen
MC
Borrego
Juan J
JJ
Alvarez
M Carmen
MC
Béjar
Julia
J
D000818:Animals; D019201:Aquabirnavirus / Q000276:immunology*; D018175:Birnaviridae Infections / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D002460:Cell Line; D002470:Cell Survival; D005393:Fish Diseases / Q000276:immunology* / Q000473:pathology; D005412:Flatfishes / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation / Q000276:immunology*; D015967:Gene Expression Regulation, Viral / Q000276:immunology; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000096:biosynthesis; D064547:Myxovirus Resistance Proteins; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D012474:Salmon; D013997:Time Factors; D014162:Transfection / Q000662:veterinary; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics
D064547:Myxovirus Resistance Proteins; D012367:RNA, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins; D019204:GTP-Binding Proteins