title: 2DE identification of proteins exhibiting turnover and phosphorylation dynamics during sea urchin egg activation.
abstract: The animal egg is a unique quiescent cell, prepackaged with maternal mRNAs and proteins that have functions in early development. Rapid, transient signaling at fertilization alters egg physiology, resulting in Ca(2+) release from the endoplasmic reticulum (ER) and cytoplasmic alkalinization. These events trigger the zygote developmental program through initiation of DNA synthesis and entry into mitosis. Post-translational modifications of maternal proteins are responsible for the spatio-temporal regulation that orchestrates egg activation. We used functional proteomics to identify the candidate maternal proteins involved in egg activation and early development. As the first step of this analysis, we present the data on the baseline maternal proteome, in particular, on proteins exhibiting changes in abundance and in phosphorylation state upon egg activation. We identify 94 proteins that were stable, reproducibly displayed a shift in isoelectric point, or changed in relative abundance at specific times after activation. The identities of these proteins were determined by quadrupole time-of-flight tandem mass spectrometry. The set of the most dynamic proteins appear to be enriched in intermediary metabolism proteins, cytoskeletal proteins, gamete associated proteins and proteins that have Ca(2+) mediated activities.
authors: Department of MCD Biology and Marine Science Institute, University of California, Santa Barbara, CA 93106-9610, USA. roux@stanford.edu
authors: Michelle M
authors: MM
authors: Roux
authors: Monte J
authors: MJ
authors: Radeke
authors: Manisha
authors: M
authors: Goel
authors: Arcady
authors: A
authors: Mushegian
authors: Kathy R
authors: KR
authors: Foltz
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005306:Fertilization / Q000235:genetics / Q000502:physiology; D016678:Genome; D007526:Isoelectric Point; D008297:Male; D008954:Models, Biological; D010063:Ovum / Q000166:cytology / Q000502:physiology*; D010750:Phosphoproteins / Q000032:analysis* / Q000378:metabolism; D010766:Phosphorylation; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics; D015203:Reproducibility of Results; D012617:Sea Urchins / Q000235:genetics / Q000502:physiology*; D013084:Sperm-Ovum Interactions / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology; D053719:Tandem Mass Spectrometry
chemical_list: D010750:Phosphoproteins; D020543:Proteome