Smart hydrogel particles: biomarker harvesting: one-step affinity purification, size exclusion, and protection against degradation.
Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and (c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis.
Luchini
Alessandra
A
CRO-IRCCS National Cancer Institute, Aviano, Italy.
Geho
David H
DH
Bishop
Barney
B
Tran
Duy
D
Xia
Cassandra
C
Dufour
Robert L
RL
Jones
Clinton D
CD
Espina
Virginia
V
Patanarut
Alexis
A
Zhou
Weidong
W
Ross
Mark M
MM
Tessitore
Alessandra
A
Petricoin
Emanuel F
EF
Liotta
Lance A
LA
D015415:Biomarkers; D002846:Chromatography, Affinity / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods*; D020100:Hydrogels; D006868:Hydrolysis
D015415:Biomarkers; D020100:Hydrogels