title: Single-molecule detection with a two-photon fluorescence microscope with fast-scanning capabilities and polarization sensitivity.
abstract: We describe a laser-scanning two-photon fluorescence microscope that is capable of observing single molecules with excellent temporal resolution and three-dimensional spatial resolution. To demonstrate the capabilities of the instrument we present single-molecule fluorescence data obtained in several different scanning modes. In addition, a polarization-sensitive detection scheme can provide detailed three-dimensional information about the orientations of molecules in any of these scanning modes.
authors: R A
authors: RA
authors: Farrer
authors: M J
authors: MJ
authors: Previte
authors: C E
authors: CE
authors: Olson
authors: L A
authors: LA
authors: Peyser
authors: J T
authors: JT
authors: Fourkas
authors: P T
authors: PT
authors: So