title: The stem cell marker prominin-1/CD133 on membrane particles in human cerebrospinal fluid offers novel approaches for studying central nervous system disease.
abstract: Cerebrospinal fluid (CSF) is routinely used for diagnosing and monitoring neurological diseases. The CSF proteins used so far for diagnostic purposes (except for those associated with whole cells) are soluble. Here, we show that human CSF contains specific membrane particles that carry prominin-1/CD133, a neural stem cell marker implicated in brain tumors, notably glioblastoma. Differential and equilibrium centrifugation and detergent solubility analyses showed that these membrane particles were similar in physical properties and microdomain organization to small membrane vesicles previously shown to be released from neural stem cells in the mouse embryo. The levels of membrane particle-associated prominin-1/CD133 declined during childhood and remained constant thereafter, with a remarkably narrow range in healthy adults. Glioblastoma patients showed elevated levels of membrane particle-associated prominin-1/CD133, which decreased dramatically in the final stage of the disease. Hence, analysis of CSF for membrane particles carrying the somatic stem cell marker prominin-1/CD133 offers a novel approach for studying human central nervous system disease.
authors: Tissue Engineering Laboratories, Biotechnology Center (BIOTEC), Technische Universität Dresden, Tatzberg 47-51, 01307 Dresden, Germany. hagen.huttner@uk-erlangen.de
authors: Hagen B
authors: HB
authors: Huttner
authors: Peggy
authors: P
authors: Janich
authors: Martin
authors: M
authors: Köhrmann
authors: József
authors: J
authors: Jászai
authors: Florian
authors: F
authors: Siebzehnrubl
authors: Ingmar
authors: I
authors: Blümcke
authors: Meinolf
authors: M
authors: Suttorp
authors: Manfred
authors: M
authors: Gahr
authors: Daniela
authors: D
authors: Kuhnt
authors: Christopher
authors: C
authors: Nimsky
authors: Dietmar
authors: D
authors: Krex
authors: Gabriele
authors: G
authors: Schackert
authors: Kai
authors: K
authors: Löwenbrück
authors: Heinz
authors: H
authors: Reichmann
authors: Eric
authors: E
authors: Jüttler
authors: Werner
authors: W
authors: Hacke
authors: Peter D
authors: PD
authors: Schellinger
authors: Stefan
authors: S
authors: Schwab
authors: Michaela
authors: M
authors: Wilsch-Bräuninger
authors: Anne-Marie
authors: AM
authors: Marzesco
authors: Denis
authors: D
authors: Corbeil
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000134:cerebrospinal fluid*; D015415:Biomarkers / Q000378:metabolism; D018938:Caco-2 Cells; D002493:Central Nervous System Diseases / Q000134:cerebrospinal fluid*; D005909:Glioblastoma / Q000134:cerebrospinal fluid; D006023:Glycoproteins / Q000134:cerebrospinal fluid*; D006801:Humans; D010455:Peptides / Q000134:cerebrospinal fluid*; D012015:Reference Standards; D013234:Stem Cells / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D015415:Biomarkers; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse