Vulgarenol, a sesquiterpene isolated from Magnolia grandiflora, induces nitric oxide synthases II and III overexpression in guinea pig hearts.
Vulgarenol, a sesquiterpene isolated from Magnolia grandiflora flower petals, decreased coronary vascular resistance in the Langendorff isolated and perfused heart model, when compared to the control group [(15.2 x 10(7) +/- 1.0 x 10(7)) dyn s cm(-5) vs. (36.8 x 10(7) +/- 1.2 x 10(7)) dyn s cm(-5)]. Our data suggest that this coronary vasodilator effect probably involved inducible and endothelial nitric oxide synthase overexpression (6.8 and 4.2 times over control, respectively), which correlated with increases in nitric oxide release [(223 +/- 9) pmol mL(-1) vs. (61 +/- 11) pmol mL(-1)] and in cyclic guanosine monophosphate production [(142 +/- 8) pmol mg(-1) of tissue vs. (44 +/- 10) pmol mg(-1) of tissue], as compared to control values. This effect was antagonized by 3 microm gadolinium(III) chloride, 100 microM N-nitro-L-arginine methyl ester, and 10 microM 1H-[1,2,4]oxadiazolo[4,2-a]quinoxalin-1-one. Hence, the vulgarenol-elicited coronary vasodilator effect could be mediated by the nitric oxide-soluble guanylyl cyclase pathway.
del Valle-Mondragón
Leonardo
L
Departamento de Farmacología, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano #1, Sección XVI, 14080, Tlalpan, DF México. leonardodvm65@hotmail.com
Tenorio-López
Fermin Alejandro
FA
Zarco-Olvera
Gabriela
G
Pastelín-Hernández
Gustavo
G
D000818:Animals; D006152:Cyclic GMP / Q000378:metabolism; D035264:Flowers / Q000737:chemistry; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects; D031566:Magnolia / Q000737:chemistry*; D009206:Myocardium / Q000201:enzymology*; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology*
C526553:3,4,9-trihydroxy-9-methyl-3a,5,5a,9,9a,9b-hexahydronaphtho(1,2-b)furan-2,6(3H,4H)-dione; D012717:Sesquiterpenes; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D006152:Cyclic GMP