Analysis of the cerebral transcriptome in mice subjected to traumatic brain injury: importance of IL-6.
Traumatic brain injury is one of the leading causes of incapacity and death among young people. Injury to the brain elicits a potent inflammatory response, comprising recruitment of inflammatory cells, reactive astrogliosis and activation of brain macrophages. Under the influence of presumably several cytokines and growth factors, a cascade of events is activated that result ultimately in increased oxidative stress and tissue damage, but also in activation of counterregulatory factors and tissue regeneration. The complexity of this response is being unraveled by high-throughput methodologies such as microarrays. The combination of these modern techniques with the comparison of normal and genetically modified mice boosts the significance of the results obtained. With this approach, we have demonstrated that a cytokine such as interleukin-6 is one of the key players in the response of the brain to injury.
Quintana
Albert
A
Institute of Neurosciences and Department of Cellular Biology, Physiology and Immunology, Animal Physiology Unit, Faculty of Sciences, Autonomous University of Barcelona, Bellaterra, Spain.
Giralt
Mercedes
M
Molinero
Amalia
A
Campbell
Iain L
IL
Penkowa
Milena
M
Hidalgo
Juan
J
D000818:Animals; D001253:Astrocytes / Q000276:immunology; D001930:Brain Injuries / Q000150:complications* / Q000276:immunology* / Q000503:physiopathology; D002540:Cerebral Cortex / Q000378:metabolism / Q000503:physiopathology; D004660:Encephalitis / Q000235:genetics* / Q000276:immunology* / Q000503:physiopathology; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology*; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D005911:Gliosis / Q000235:genetics / Q000276:immunology / Q000503:physiopathology; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D005904:Glial Fibrillary Acidic Protein; D015850:Interleukin-6; D012333:RNA, Messenger