[Adhesion and inhibiting adhesion of Helicobacter pylori to SGC-7901 cell in vitro by four Lactobacilli from human].
OBJECTIVE
To investigate the adherence ability of four Lactobacilli to gastric epithelial cell SGC-7901 and the ability of inhibiting the adherence of Helicobacter pylori to the cells.


METHODS
The light microscope and scanning electron microscope were used to detect Lactobacilli that adhering to SGC-7901 cells after co-culturing for 2 hours. Using FITC-conjuncted antibody, the adhered Helicobacter pylori density was evaluated under fluorescence microscope after pretreatment with live or nonviable Lactobacilli.


RESULTS
Four Lactobacilli's live and nonviable stains could adhere to SGC-7901 tightly and obviously inhibit the adherence of Helicobacter pylori, but their adherence ability was not different, excluding their live stains adherence ability. And their inhibiting ability were different, of which the best was Lac15, the worst was Lac12.


CONCLUSIONS
Four Lactobacilli can inhibit the adherence of Helicobacter pylori to gastric epithelial cell SGC-7901 in vitro, and they should be further evaluated in vivo.
Pan
Suhua
S
Panyu Entry-Exit Inspection And Quarantine Bureau, Guangzhou 511400, China. pansh7512@163.com
Yu
Qian
Q
Bie
Mingjiang
M
Liu
Xiang
X
D000230:Adenocarcinoma / Q000382:microbiology / Q000473:pathology; D000898:Antibiosis / Q000502:physiology*; D001422:Bacterial Adhesion / Q000502:physiology*; D045744:Cell Line, Tumor; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D007778:Lactobacillus / Q000302:isolation & purification / Q000502:physiology*; D013274:Stomach Neoplasms / Q000382:microbiology* / Q000473:pathology