title: [The methylation locus and frequency pattern on p16 INK4a gene promoter CpG in epidermis of patients with psoriasis].
abstract: OBJECTIVE
  To investigate the CpG methylation locus and frequency pattern on p16 INK4a gene promoter in epidermis of p16 INK4a methylated patients with psoriasis vulgaris.
  
  
  METHODS
  The DNA specimens were obtained from epidermal lesion of 50 plaque psoriatic patients. Methylation specific PCR and DNA sequencing were used to detect the frequency and locus of methylation in p16 INK4a gene promoter region.
  
  
  RESULTS
  Approximately 50% CpG was methylated in p16 INK4a methylated patients, methylation was found in specifical locus of p16 INK4a gene promoter.
  
  
  CONCLUSIONS
  The distinct methylation pattern is showed on the p16 INK4a gene promoter region in patients with psoriasis.
authors: Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, Jiangsu, 210042 PR China.
authors: Min
authors: M
authors: Chen
authors: Pan-gen
authors: PG
authors: Cui
authors: Xu
authors: X
authors: Yao
authors: Yuan-hua
authors: YH
authors: Cao
authors: Juan-qin
authors: JQ
authors: Gong
authors: An-sheng
authors: AS
authors: Li
authors: Zhi-qiang
authors: ZQ
authors: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D018899:CpG Islands / Q000235:genetics*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation / Q000235:genetics*; D004817:Epidermis / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011565:Psoriasis / Q000235:genetics*; D016415:Sequence Alignment; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics; D055815:Young Adult
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D025803:Tumor Suppressor Protein p14ARF