title: Targeted folic acid-PEG nanoparticles for noninvasive imaging of folate receptor by MRI.
abstract: The surface of superparamagnetic iron oxide nanoparticles (SPIO) with different molecular weight of poly(ethylene glycol) (PEG) and folic acid (FA) were synthesized. The SPIO-PEG-FA nanoparticles are well-dispersed and have good stability in various pH solutions. The lack of hysterestis and remanence at ambient temperatures is characteristic of superparamagnetic materials for SPIO-PEG-FA. The uptake by macrophage for SPIO-PEG-FA is lower than that of Feridex I.V. even at higher concentration. Internalization of SPIO-PEG-FA in targeted cells (KB cells) was observed by flow-cytometric analysis and in vitro MR imaging. The intensity change of positive KB cell tumor (-20 to 25%) is significantly lower than that of negative HT-1080 cell tumor from precontrast to postcontrast images of the tumor by in vivo MR imaging. These preliminary results demonstrated that SPIO-PEG-FA have the ability to target folate receptor.
authors: Faculty of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors: Ting-Jung
authors: TJ
authors: Chen
authors: Tsan-Hwang
authors: TH
authors: Cheng
authors: Yu-Chin
authors: YC
authors: Hung
authors: Kuei-Tang
authors: KT
authors: Lin
authors: Gin-Chung
authors: GC
authors: Liu
authors: Yun-Ming
authors: YM
authors: Wang
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry*; D003287:Contrast Media / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D005434:Flow Cytometry; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D007624:KB Cells; D008264:Macrophages / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D051379:Mice; D016513:Mice, SCID; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry*; D011956:Receptors, Cell Surface / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D023041:Xenograft Model Antitumor Assays
chemical_list: D002352:Carrier Proteins; D003287:Contrast Media; D005290:Ferric Compounds; D058973:Folate Receptors, GPI-Anchored; D011956:Receptors, Cell Surface; C000499:ferric oxide; D011092:Polyethylene Glycols; D005492:Folic Acid