title: [Gene screening in five Chinese families with hereditary spastic paraplegia with thin corpus callosum].
abstract: OBJECTIVE
  To screen all ten genes between D15S971 and D15S1012 in five Chinese families with hereditary spastic paraplegia with thin corpus callosum (HSP-TCC).
  
  
  METHODS
  DNA samples from 5 HSP-TCC families were screened for mutations in AK128197, MGC14798, HH114, MEIS2, MGC35118, SPRED1, AK128458, FLJ38426, RASGRP1 and AK093014 on chromosome 15q13-15 between microsatellites D15S971 and D15S1012 by polymerase chain reaction, direct sequencing and cosegreagation analysis.
  
  
  RESULTS
  No disease-causing mutations were found in the 10 genes, but 13 polymorphisms were identified in which two were novel.
  
  
  CONCLUSIONS
  This study did not support the ten genes between D15S971 and D15S1012 were the disease-causing genes of the 5 HSP-TCC families.
authors: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008 PR China.
authors: Guo-hua
authors: GH
authors: Zhao
authors: Peng
authors: P
authors: Guo
authors: Zhi-jun
authors: ZJ
authors: Ren
authors: Xiao-min
authors: XM
authors: Liu
authors: Lu
authors: L
authors: Shen
authors: Kun
authors: K
authors: Xia
authors: Bei-sha
authors: BS
authors: Tang
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics; D002884:Chromosomes, Human, Pair 15; D003337:Corpus Callosum / Q000473:pathology*; D005260:Female; D005808:Genes, Recessive; D006801:Humans; D008297:Male; D020336:Paraparesis, Spastic / Q000235:genetics; D015419:Spastic Paraplegia, Hereditary / Q000150:complications / Q000235:genetics*