title: [Biotransformation of mogroside III by human intestinal bacteria].
abstract: OBJECTIVE
  To investigate the biotransformation of mogroside III, one of the main chemical constituent in the fruits of Momordica grosvenori Swingle, by the crude enzymes of human intestinal bacteria, and determine the structure of biotransformation products and provide scientific basis for absorption evaluation of primary mogroside III in human intestine.
  
  
  METHODS
  The mogroside III was incubated with crude enzymes of human intestinal bacteria under the anaerobic environment and 37 degrees C condition to transform mogroside III. The biotransformation products were isolated and purified by silica gel column chromatography, and structurally determined by infra-red (IR) and nuclear magnetic resonance (NMR) spectrometry and mass spectroscopy (MS) techniques.
  
  
  RESULTS
  Mogroside III was converted to mogroside II(A1) and mogrol by successive deglycosylation at C-3 of the glucosyl group and C-24 of the gentiobiosyl group.
  
  
  CONCLUSIONS
  The human intestinal bacteria showed potent ability to transform mogroside III to release secondary glycoside mogroside II(A1) and aglycone mogrol.
authors: Department of Natural Medicines, Peking University School of Pharmaceutical Sciences, Beijing 100083, China. xwyang@bjmu.edu.cn
authors: Xiu Wei
authors: XW
authors: Yang
authors: Jian Ye
authors: JY
authors: Zhang
authors: Wei
authors: W
authors: Xu
mesh_terms: D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001711:Biotransformation / Q000187:drug effects*; D015169:Colony Count, Microbial; D005638:Fruit / Q000737:chemistry; D006027:Glycosides / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D029775:Momordica / Q000737:chemistry*; D014315:Triterpenes / Q000378:metabolism*
chemical_list: D006027:Glycosides; D014315:Triterpenes