Biotransformation of (+)-catechin into taxifolin by a two-step oxidation: primary stage of (+)-catechin metabolism by a novel (+)-catechin-degrading bacteria, Burkholderia sp. KTC-1, isolated from tropical peat.
Peat contains various persistent compounds derived from plant materials. We isolated a novel (+)-catechin-degrading bacterium, Burkholderia sp. KTC-1 (KTC-1), as an example of a bacterium capable of degrading persistent aromatic compounds present in tropical peat. This bacterium was isolated by an enrichment technique and grew on (+)-catechin as the sole carbon source under acidic conditions. The reaction of a crude enzyme extract and a structural study of its products showed that (+)-catechin is biotransformed into taxifolin during the preliminary stages of its metabolism by KTC-1. HPLC analysis showed that this transformation occurs in two oxidation steps: 4-hydroxylation and dehydrogenation. Furthermore, both (+)-catechin 4-hydroxylanase and leucocyanidin 4-dehydrogenase were localized in the cytosol of KTC-1. This is the first report on biotransformation of (+)-catechin into taxifolin via leucocyanidin by an aerobic bacterium. We suggest that tropical peat could become a unique resource for microorganisms that degrade natural aromatic compounds.
Matsuda
Motoki
M
Graduate School of Agriculture, Hokkaido University, N9W9, Kita-ku, Sapporo 060-8589, Japan.
Otsuka
Yuichiro
Y
Jin
Shigeki
S
Wasaki
Jun
J
Watanabe
Jun
J
Watanabe
Toshihiro
T
Osaki
Mitsuru
M
D001711:Biotransformation; D019117:Burkholderia / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D002392:Catechin / Q000378:metabolism*; D044948:Flavonols / Q000378:metabolism; D010084:Oxidation-Reduction; D011794:Quercetin / Q000031:analogs & derivatives* / Q000378:metabolism; D012988:Soil Microbiology; D013045:Species Specificity
D044948:Flavonols; D002392:Catechin; D011794:Quercetin; C003377:taxifolin