Expression of BCR-ABL1 oncogene relative to ABL1 gene changes overtime in chronic myeloid leukemia.
Using a quantitative single nucleotide polymorphism (SNP) assay we have investigated the changes in the expression of the BCR-ABL1 oncogene relative to the wild-type ABL1 and BCR alleles in cells from chronic myeloid leukemia (CML) patients not responding to therapy. The results show a progressive increase in the BCR-ABL1 oncogene expression at the expense of decreased expression of the ABL1 allele, not involved in the fusion. No relative changes in the expression of the two BCR alleles were found. These results demonstrate that allele-specific changes in gene expression, with selective, progressive silencing of the wild-type ABL1 allele in favor of the oncogenic BCR-ABL1 allele occur in CML patients with therapy-resistant disease.
Gupta
Manu
M
Molecular Medicine, Department of Medical Sciences, Uppsala University, Sweden.
Milani
Lili
L
Hermansson
Monica
M
Simonsson
Bengt
B
Markevärn
Berit
B
Syvänen
Ann Christine
AC
Barbany
Gisela
G
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D016044:Fusion Proteins, bcr-abl / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D016313:Genes, abl; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism*; D014407:Tumor Cells, Cultured
C513733:ABI1 protein, human; D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; D016044:Fusion Proteins, bcr-abl