title: Porous beta-tricalcium phosphate/collagen composites prepared in an alkaline condition.
abstract: Bone substitute materials with natural bone-like structure are considered to be favorable for bone regeneration. In this work, porous beta-tricalcium phosphate (beta-TCP)/collagen composite consisting of bone-like microstructural units was prepared using nanosized beta-TCP particles and alkaline-disassembled collagen. The resulting composite showed a good interconnecting porous structure with approximately 90% porosity and 100 approximately 300 microm pore size. The pore walls were dense, and the combination status of collagen and nanosized beta-TCP particles demonstrated that nanosized beta-TCP particles tightly connected collagen microfibrils as a bone-like microstructural unit. MTT and alkaline phosphatase (ALP) assays showed that the porous composite had enhanced effects on cellular proliferation and activity of osteoblast compared with a control of pure collagen. It is suggested that the adoption of nanosized beta-TCP particles is a main contribution to the formation of the composite with a bone-like microstructural unit, and the unique microstructure could be a main role for the composite to have the positive influence on osteoblast cell proliferation.
authors: Department of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors: Chao
authors: C
authors: Zou
authors: Wenjian
authors: W
authors: Weng
authors: Kui
authors: K
authors: Cheng
authors: Piyi
authors: P
authors: Du
authors: Ge
authors: G
authors: Shen
authors: Gaorong
authors: G
authors: Han
authors: Binggang
authors: B
authors: Guan
authors: Weiqi
authors: W
authors: Yan
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000704:Analysis of Variance; D018786:Bone Substitutes / Q000138:chemical synthesis*; D002130:Calcium Phosphates / Q000138:chemical synthesis*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D016062:Porosity; D013499:Surface Properties; D023822:Tissue Engineering; D014961:X-Ray Diffraction
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D003094:Collagen; D000469:Alkaline Phosphatase