An integrated physiological and genetic approach to the dynamics of FtsZ targeting and organisation in a moss, Physcomitrella patens.
Plant FtsZ (filamentous temperature-sensitive Z) proteins are regarded as descendants of prokaryotic cell division proteins. We could show previously that four FtsZ isoforms of the moss Physcomitrella patens assemble into, and interact in, distinct structures inside the chloroplasts and in the cytosol. Their organisation and localisation patterns indicate an involvement in chloroplast and cell division and in the maintenance of chloroplast shape and integrity. The cellular processes of chloroplast division and maintenance of chloroplast shape were disturbed either by application of the beta-lactam antibiotic ampicillin or by a mutation that presumably affects signal transduction of the plant hormone cytokinin. When cells of these plants were analysed microscopically, there was no indication that cytosolic functions of FtsZ proteins were affected. Furthermore, FtsZ proteins continued to build three-dimensional plastoskeleton networks, even in considerably enlarged or malformed chloroplasts. On the other hand, macrochloroplast formation promoted the localisation of FtsZ proteins in filaments that emanate from the plastids and, therefore, most likely represent stromules. Annular FtsZ structures that are regarded as essential components of the division apparatus were absent from macrochloroplasts of ampicillin-treated cells. Thus, the distribution of FtsZ proteins after inhibition of chloroplast division further strengthens our hypothesis on the functions of distinct isoforms. In addition, the results provide further insight into the regulation of protein targeting and dynamics of plastoskeletal elements.
Suppanz
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Plant Biotechnology, Faculty of Biology, University of Freiburg, Freiburg, Federal Republic of Germany.
Sarnighausen
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Reski
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D000667:Ampicillin / Q000494:pharmacology; D029681:Arabidopsis Proteins; D019068:Bryopsida / Q000166:cytology / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D018087:Plastids / Q000187:drug effects / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D011523:Protoplasts / Q000187:drug effects / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects
D029681:Arabidopsis Proteins; C095113:FTSZ protein, Arabidopsis; D010940:Plant Proteins; D020033:Protein Isoforms; D000667:Ampicillin