title: Effect of reversed heme orientation on circular dichroism and cooperative oxygen binding of human adult hemoglobin.
abstract: We found that recombinant human adult hemoglobin (rHb A) expressed in Escherichia coli showed heterogeneity of components with the intensity of a positive CD band at 260 nm and that it could be resolved into three components (SP-1, SP-2, and SP-3) by SP-Sepharose column chromatography. 1H NMR revealed that SP-1 is identical with native Hb A, while SP-2 and SP-3 largely contain the reversed heme isomer in both the alpha and beta subunits, with contents of approximately 50 and >80% in SP-2 and SP-3, respectively. Rotation of the heme 180 degrees about the 5,15-meso axis (reversed heme) causes an interexchange of the methyl groups at positions 2 and 7 with the vinyl groups at positions 8 and 3, respectively. To examine the effect of the modification of the heme-protein contact on the structure and function of Hb A, we compared the 1H NMR, CD, and oxygen binding properties of the three components with those of native Hb A. Native Hb A exhibits a distinct positive CD band in both the near-UV and Soret regions, but rHb A with reversed heme exhibits a very weak positive CD band at 260 nm and a prominent negative CD band in the Soret region. Cooperativity, as measured by Hill's n value, decreased from 3.18 (SP-1) to 2.94 (SP-2) to 2.63 (SP-3) with an increase in the reversed heme orientation. The effect of an allosteric effector, inositol hexaphosphate (IHP), on the oxygen binding properties was also reduced in rHb A with reversed heme. These results indicate that changes in the heme-globin contact exert a discernible influence on CD spectra and cooperative oxygen binding.
authors: Research Center for Micro-Nano Technology, Hosei University, Tokyo 184-0003, Japan. masako.nagai.gv@ k.hosei.ac.jp
authors: Masako
authors: M
authors: Nagai
authors: Yukifumi
authors: Y
authors: Nagai
authors: Yayoi
authors: Y
authors: Aki
authors: Kiyohiro
authors: K
authors: Imai
authors: Yoshinao
authors: Y
authors: Wada
authors: Shigenori
authors: S
authors: Nagatomo
authors: Yasuhiko
authors: Y
authors: Yamamoto
mesh_terms: D000328:Adult; D002942:Circular Dichroism; D006418:Heme / Q000737:chemistry* / Q000378:metabolism*; D006441:Hemoglobin A / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008968:Molecular Conformation; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D010100:Oxygen / Q000378:metabolism*; D021122:Protein Subunits; D011522:Protons; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012685:Sepharose
chemical_list: D021122:Protein Subunits; D011522:Protons; D011994:Recombinant Proteins; D006418:Heme; D012685:Sepharose; D006441:Hemoglobin A; D010100:Oxygen