title: The Drosophila NURF remodelling and the ATAC histone acetylase complexes functionally interact and are required for global chromosome organization.
abstract: Drosophila Gcn5 is the catalytic subunit of the SAGA and ATAC histone acetylase complexes. Here, we show that mutations in Gcn5 and the ATAC component Ada2a induce a decondensation of the male X chromosome, similar to that induced by mutations in the Iswi and Nurf301 subunits of the NURF nucleosome remodelling complex. Genetic studies as well as transcript profiling analysis indicate that ATAC and NURF regulate overlapping sets of target genes during development. In addition, we find that Ada2a chromosome binding and histone H4-Lys12 acetylation are compromised in Iswi and Nurf301 mutants. Our results strongly suggest that NURF is required for ATAC to access the chromatin and to regulate global chromosome organization.
authors: Department of Developmental Biology/CNRS URA 2578, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France.
authors: Clément
authors: C
authors: Carré
authors: Anita
authors: A
authors: Ciurciu
authors: Orbán
authors: O
authors: Komonyi
authors: Caroline
authors: C
authors: Jacquier
authors: Delphine
authors: D
authors: Fagegaltier
authors: Josette
authors: J
authors: Pidoux
authors: Hervé
authors: H
authors: Tricoire
authors: Laszlo
authors: L
authors: Tora
authors: Imre M
authors: IM
authors: Boros
authors: Christophe
authors: C
authors: Antoniewski
mesh_terms: D000107:Acetylation; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D017344:Genes, Insect; D051548:Histone Acetyltransferases / Q000378:metabolism*; D007814:Larva; D008297:Male; D046912:Multiprotein Complexes / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D021122:Protein Subunits / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014960:X Chromosome / Q000378:metabolism*; D000099224:p300-CBP-Associated Factor
chemical_list: D000251:Adenosine Triphosphatases; D029721:Drosophila Proteins; D051548:Histone Acetyltransferases; D046912:Multiprotein Complexes; D021122:Protein Subunits; D014157:Transcription Factors; C086468:ISWI protein; C470557:GCN5 protein, Drosophila; D000099224:p300-CBP-Associated Factor