title: Linear growth in relation to the circulating concentrations of insulin-like growth factor I, parathyroid hormone, and 25-hydroxy vitamin D in children with nutritional rickets before and after treatment: endocrine adaptation to vitamin D deficiency.
abstract: The objective of the study was to determine the degree of linear growth retardation of patients with vitamin D deficiency rickets at presentation and the magnitude of catch-up growth in relation to their calcium (Ca) homeostasis and hormones affecting it before and after treatment. This prospective study recorded the anthropometric data and measured the circulating 25-hydroxy vitamin D (25-OH-D), insulin-like growth factor I (IGF-I), parathyroid hormone, Ca, phosphate, and alkaline phosphatase concentrations in 46 infants and children with nutritional (vitamin D deficiency) rickets before and 6 months or more after treatment with one intramuscular injection of vitamin D3 megadose (300000 IU). Forty normal age- and sex-matched children were included as controls for the auxological data. At presentation, patients' mean age = 13.1 +/- 1.1 months, length standard deviation scores (LSDS) = -1.5 +/- 0.2, and body mass index = 16.3 +/- 0.85. They were significantly shorter and had markedly lower growth velocity standard deviation scores (GVSDS) compared with normal controls (LSDS = 0.25 +/- 0.18 and 0.31 +/- 0.22, respectively). Six months after treatment, the LSDS increased significantly in patients to -0.45 +/- 0.13, with a significantly increased GVSDS (2.76 +/- 0.45) and body mass index (16.9 +/- 0.65). They were still shorter but with significantly higher GVSDS compared with normal controls. Serum Ca and phosphate concentrations increased from 2.07 +/- 0.25 and 1.23 +/- 0.24 mmol/L, respectively, before treatment to 2.44 +/- 0.2 and 1.94 +/- 0.2 mmol/L, respectively, after treatment. Serum alkaline phosphatase and parathyroid hormone concentrations decreased from 1183 +/- 219 U/L and 294 +/- 87 pg/mL, respectively, before treatment to 334 +/- 75 U/L and 35.2 +/- 15.2 pg/mL, respectively, after treatment. The 25-OH-D level increased from 4.5 +/- 0.56 ng/mL before treatment to 44.5 +/- 3.7 ng/mL after treatment. Circulating concentrations of IGF-I increased significantly after treatment (52.2 +/- 18.9 ng/mL) vs before treatment (26.6 +/- 12.8 ng/mL). The 25-OH-D concentrations were correlated significantly with the IGF-I levels before and after treatment (r = 0.603 and r = 0.59, respectively; P < .001). The GVSDS after treatment was correlated with the increase of IGF-I and 25-OH-D levels (r = 0.325 and r= 0.314, respectively; P < .01). These data denote that the accelerated linear growth after treatment of nutritional vitamin D deficiency is mediated through activation of the growth hormone/IGF-I system and suggests an important role of vitamin D as a link between the proliferating cartilage cells of the growth plate and growth hormone/IGF-I secretion. Three different sequential stages of vitamin D deficiency can be recognized according to the clinical/radiological, biochemical, and hormonal data of patients at presentation.
authors: Division of Pediatric Endocrinology, Hamad Medical Centre, P.O. box 3050, Doha, Qatar. atsoliman@yahoo.com
authors: Ashraf T
authors: AT
authors: Soliman
authors: Fauzia
authors: F
authors: Al Khalaf
authors: Noura
authors: N
authors: Alhemaidi
authors: Maryam
authors: M
authors: Al Ali
authors: Mahmoud
authors: M
authors: Al Zyoud
authors: Khaled
authors: K
authors: Yakoot
mesh_terms: D000293:Adolescent; D001827:Body Height; D015992:Body Mass Index; D002118:Calcium / Q000097:blood; D002648:Child; D002675:Child, Preschool; D006128:Growth / Q000502:physiology; D006801:Humans; D006887:Hydroxycholecalciferols / Q000097:blood*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000097:blood*; D018579:Patient Selection; D010710:Phosphates / Q000097:blood; D011780:Qatar; D012279:Rickets / Q000097:blood*; D014808:Vitamin D Deficiency / Q000097:blood*
chemical_list: D006887:Hydroxycholecalciferols; D010281:Parathyroid Hormone; D010710:Phosphates; D007334:Insulin-Like Growth Factor I; D002118:Calcium