Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin.
HA3, a 70 kDa haemagglutinating protein, is a precursor form of HA3a and HA3b, the subcomponents of Clostridium botulinum type C 16S progenitor toxin. In this report, recombinant HA3 protein was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.6 A resolution and the crystal belonged to the hexagonal space group P6(3). Matthews coefficient and self-rotation function calculations indicate that there is probably one molecule of HA3 in the asymmetric unit. A search for heavy-atom derivatives has been undertaken.
Nakamura
Toshio
T
Department of Applied Biological Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
Tonozuka
Takashi
T
Kotani
Mao
M
Obata
Kanae
K
Oguma
Keiji
K
Nishikawa
Atsushi
A
D001905:Botulinum Toxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003014:Clostridium botulinum / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003460:Crystallization; D014961:X-Ray Diffraction
D001905:Botulinum Toxins; C096324:botulinum toxin type C