title: Investigating the genetic circuitry of mastermind in Drosophila, a notch signal effector.
abstract: Notch signaling regulates multiple developmental processes and is implicated in various human diseases. Through use of the Notch transcriptional co-activator mastermind, we conducted a screen for Notch signal modifiers using the Exelixis collection of insertional mutations, which affects approximately 50% of the Drosophila genome, recovering 160 genes never before associated with Notch, extending the previous roster of genes that interact functionally with the Notch pathway and mastermind. As the molecular identity for most recovered genes is known, gene ontology (GO) analysis was applied, grouping genes according to functional classifications. We identify novel Notch-associated GO categories, uncover nodes of integration between Notch and other signaling pathways, and unveil groups of modifiers that suggest the existence of Notch-independent mastermind functions, including a conserved ability to regulate Wnt signaling.
authors: Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
authors: Mark W
authors: MW
authors: Kankel
authors: Gregory D
authors: GD
authors: Hurlbut
authors: Geeta
authors: G
authors: Upadhyay
authors: Vijay
authors: V
authors: Yajnik
authors: Barry
authors: B
authors: Yedvobnick
authors: Spyros
authors: S
authors: Artavanis-Tsakonas
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics*; D053263:Gene Regulatory Networks; D017344:Genes, Insect; D049750:Genome, Insect; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics*; D051880:Receptors, Notch / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics*; D051153:Wnt Proteins
chemical_list: D029721:Drosophila Proteins; D009687:Nuclear Proteins; D051880:Receptors, Notch; D051153:Wnt Proteins; C085316:mam protein, Drosophila