title: Malignant adenomyoepithelial tumor of the breast: multi-immunolabeling technique and detailed immunophenotypic study.
abstract: Biphasic ductal and myoepithelial lesions of the breast are uncommon. A majority of these rare lesions behave in a benign fashion. Malignancy is rare, and preferentially involves the epithelial component. Malignancy in both epithelial and myoepithelial components is even rarer. We report such a case, employing the use of a range of cytokeratins and myoepithelial markers to delineate the extent of each component. In addition, we apply the relatively novel technique of multi-immunolabeling combined with Adobe Photoshop imaging to highlight the different components of the neoplasm on the same tissue section. These ancillary tests can provide much needed information about the contribution of epithelial or myoepithelial components to malignant tumors, hence providing the gateway to further study into their histogenesis and natural history.
authors: Department of Pathology, National University of Singapore, Singapore. patngame@nus.edu.sg
authors: Min En
authors: ME
authors: Nga
authors: Kiat Hon
authors: KH
authors: Lim
authors: Ern-Yu
authors: EY
authors: Tan
authors: Patrick
authors: P
authors: Chan
authors: Soo-Yong
authors: SY
authors: Tan
authors: Norman
authors: N
authors: Walford
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis; D001940:Breast / Q000737:chemistry / Q000276:immunology; D001943:Breast Neoplasms / Q000737:chemistry / Q000473:pathology*; D004847:Epithelial Cells / Q000737:chemistry; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007633:Keratins / Q000032:analysis; D008875:Middle Aged; D009208:Myoepithelioma / Q000737:chemistry / Q000473:pathology; D018193:Neoplasms, Complex and Mixed / Q000737:chemistry / Q000473:pathology*
chemical_list: D000951:Antigens, Neoplasm; D007633:Keratins