[Studies on the HPLC fingerprint spectrum of flavonoids in Gynostemma pentaphyllum].
OBJECTIVE
To establish the HPLC fingerprint spectrum of flavonoids from Gynostemma pentaphyllum.


METHODS
The HPLC conditions with TURNER Kromasil C18 column (250 x 4. 6 mm, 5 microm) was used, the mobile phase consisted of 0.4% phosphoric acid-methanol-acetonitrile with gradient elution at flow rate of 0.5 ml/min and the detection wavelength was set at 360nm.


RESULTS
Ten common characteristic peaks were taken as fingerprint peaks the presision and the retest are in accordance with the requirement.


CONCLUSIONS
The method is stable, reliable, precision and provides a scientific basis for the quality standard of Gynostemma pentaphyllum.
Lu
Jin-qing
JQ
Hubei College of Traditional Chinese Medicine, Wuhan 430061, China. ljq59169@sohu.com
Chen
Li
L
Xiao
Bo
B
Wan
Wei
W
Yu
Fan
F
Tian
Yao-ping
YP
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D029774:Gynostemma / Q000737:chemistry*; D000432:Methanol; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D000432:Methanol