title: Mitochondrial genome deletion aids in the identification of false- and true-negative prostate needle core biopsy specimens.
abstract: We report the usefulness of a 3.4-kb mitochondrial genome deletion (3.4 mtdelta) for molecular definition of benign, malignant, and proximal to malignant (PTM) prostate needle biopsy specimens. The 3.4 mtdelta was identified through long-extension polymerase chain reaction (PCR) analysis of frozen prostate cancer samples. A quantitative PCR assay was developed to measure the levels of the 3.4 mtdelta in clinical samples. For normalization, amplifications of a nuclear target and total mitochondrial DNA were included. Cycle threshold data from these targets were used to calculate a score for each biopsy sample. In a pilot study of 38 benign, 29 malignant, and 41 PTM biopsy specimens, the difference between benign and malignant core biopsy specimens was well differentiated (P & .0001), with PTM indistinguishable from malignant samples (P = .833). Results of a larger study were identical. In comparison with histopathologic examination for benign and malignant samples, the sensitivity and specificity were 80% and 71%, respectively, and the area under a receiver operating characteristic (ROC) curve was 0.83 for the deletion. In a blinded external validation study, the sensitivity and specificity were 83% and 79%, and the area under the ROC curve was 0.87. The 3.4 mtdelta may be useful in defining malignant, benign, and PTM prostate tissues.
authors: Genesis Genomics, Thunder Bay, Canada.
authors: Jennifer
authors: J
authors: Maki
authors: Kerry
authors: K
authors: Robinson
authors: Brian
authors: B
authors: Reguly
authors: Jude
authors: J
authors: Alexander
authors: Roy
authors: R
authors: Wittock
authors: Andrea
authors: A
authors: Aguirre
authors: Eleftherios P
authors: EP
authors: Diamandis
authors: Nicholas
authors: N
authors: Escott
authors: Anthony
authors: A
authors: Skehan
authors: Owen
authors: O
authors: Prowse
authors: Robert E
authors: RE
authors: Thayer
authors: M Kent
authors: MK
authors: Froberg
authors: Michael J
authors: MJ
authors: Wilson
authors: Samantha
authors: S
authors: Maragh
authors: John P
authors: JP
authors: Jakupciak
authors: Paul D
authors: PD
authors: Wagner
authors: Sudhir
authors: S
authors: Srivastava
authors: Gabriel D
authors: GD
authors: Dakubo
authors: Ryan L
authors: RL
authors: Parr
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics; D001707:Biopsy, Needle / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D005188:False Negative Reactions; D017353:Gene Deletion; D054629:Genome, Mitochondrial; D006801:Humans; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000473:pathology; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000235:genetics; D012372:ROC Curve
chemical_list: D014408:Biomarkers, Tumor; D004272:DNA, Mitochondrial; D004273:DNA, Neoplasm