title: Method to determine enantiomeric excess of glucose by nonchiral high-performance liquid chromatography using circular dichroism detection.
abstract: Using nonchiral high-performance liquid chromatography (HPLC) coupled with a circular dichroism (CD) detector, we developed a new method to determine the enantiomeric excess (e.e.) of glucose derivatized with chromophores (p-bromobenzoate). This method is suitable for microscale analysis of glycoside and saccharide. As an application of this method, the enantiomeric excess of the component glucose of a glucan was assayed.
authors: Shenyang Pharmaceutical University, Shenyang 110016, China.
authors: Shuang
authors: S
authors: Song
authors: Lu
authors: L
authors: Sun
authors: Lei
authors: L
authors: Yuan
authors: Tiemin
authors: T
authors: Sun
authors: Yun
authors: Y
authors: Zhao
authors: Wenjian
authors: W
authors: Zuo
authors: Yue
authors: Y
authors: Cong
authors: Xian
authors: X
authors: Li
authors: Jinhui
authors: J
authors: Wang
mesh_terms: D001970:Bromobenzoates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002942:Circular Dichroism / Q000379:methods*; D005936:Glucans / Q000737:chemistry; D005947:Glucose / Q000031:analogs & derivatives / Q000032:analysis*; D008968:Molecular Conformation; D015203:Reproducibility of Results; D013237:Stereoisomerism
chemical_list: D001970:Bromobenzoates; D005936:Glucans; D005947:Glucose