Analysis of the expression of coxsackievirus and adenovirus receptor in five colon cancer cell lines.
OBJECTIVE
To investigate the expression of coxsackievirus and adenovirus receptor (CAR) and adenovirus-mediated reporter gene transfer in five human colon cancer cell lines.


METHODS
Expression of CAR-specific mRNA and protein was analyzed by reverse transcriptase polymerase chain reaction and Western blotting, respectively. Adenovirus-based gene delivery was evaluated by infection of cells with adenoviral vector carrying the green fluorescent protein (GFP) gene.


RESULTS
All the colon cancer cell lines examined (HT29, LS180, SW480, SW948 and SW1116) expressed CAR full-length mRNA and an alternatively-spliced variant that lacks the transmembrane coding exon. All cell lines were detected as CAR-positive by Western blot analysis. Further, all cells we examined were efficiently infected with adenoviral vector-GFP.


CONCLUSIONS
The data indicated that the five colon cancer cell lines tested expressed adenovirus primary receptor and could be efficiently infected by adenoviral vectors. Therefore, these cell lines will be useful for adenovirus-based gene transfer and research.
Abdolazimi
Yassan
Y
Department of Medical Genetics, Medical Sciences/University of Tehran, PO Box 14155-6447, Tehran, Iran.
Mojarrad
Majid
M
Pedram
Mehrdad
M
Modarressi
Mohammad Hossein
MH
D000256:Adenoviridae / Q000235:genetics; D017398:Alternative Splicing; D000818:Animals; D016466:CHO Cells; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism*; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D006224:Cricetinae; D003412:Cricetulus; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D019073:HT29 Cells; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D012333:RNA, Messenger; D011991:Receptors, Virus; D049452:Green Fluorescent Proteins