Freeze-drying fungi using a shelf freeze-drier.
Lyophilization, the removal of water by freezing and then volatilization at low pressure and temperature, has been employed as a standard long-term preservation method for many filamentous fungi. The method outlined involves the use of standard shelf freeze-drying and skimmed milk as a suspending solution/lyoprotectant. This approach has been employed to freeze-dry the majority of the 50,000 fungal strains that have been successfully lyophilized at the Centraal bureau voor Schimmelcultures (CBS) culture collection (http://www.cbs.knaw.nl/).
Tan
C Shu-hui
CS
Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands.
van Ingen
Cor W
CW
Stalpers
Joost A
JA
D000818:Animals; D002417:Cattle; D003451:Cryoprotective Agents / Q000737:chemistry; D005612:Freeze Drying / Q000295:instrumentation* / Q000379:methods; D005658:Fungi; D008892:Milk / Q000737:chemistry
D003451:Cryoprotective Agents