[Polymorphism of human cytomegalovirus UL134 gene in low-passage clinical isolates].
OBJECTIVE
Human cytomegalovirus (HCMV) displays genetic polymorphisms. Nineteen open reading frames (ORFs, UL133-UL151) found in the Toledo strain of HCMV and other low-passage clinical isolates may be essential for viral infection. This study aimed to analyze the polymorphism of HCMV UL134 gene in clinical isolates and explore the relationship between the polymorphism and HCMV infection.


METHODS
PCR was performed to amplify entire UL134 region in 32 clinical isolates, which had been proven as HCMV-DNA positive by FQ-PCR. PCR products were sequenced.


RESULTS
All of the 32 isolates were amplified and sequenced successfully. HCMV UL134 gene was highly conserved in the clinical isolates. UL134 ORF and its predicted protein in the clinical strains displayed 96.4%-98.3% nucleotide identity and 92.7%-94.9% amino acid identity respectively compared to those in the Toledo strain. A new posttranslational modification site, sulfationcamp (SUL) site, was found in UL134 protein of all of the clinical isolates except 35j.


CONCLUSIONS
HCMV UL134 gene in clinical isolates was highly conserved. No substantial relation was found between UL134 gene and HCMV infectious diseases.
Ma
Yan-Ping
YP
Polymorphism of human cytomegalovirus UL134 gene in low-passage clinical isolates.
Ruan
Qiang
Q
He
Rong
R
Qi
Ying
Y
Sun
Zheng-Rong
ZR
Ji
Yao-Hua
YH
Huang
Yu-Jing
YJ
Liu
Qing
Q
Chen
Shu-Rong
SR
Wang
Ji-Dong
JD
D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000821:virology*; D005814:Genes, Viral; D006801:Humans; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins