title: Affinity of dinucleotide cap analogues for human decapping scavenger (hDcpS).
abstract: Eukaryotic cells utilize scavenger decapping enzymes to degrade cap structure following 3'-5' mRNA decay. Human DcpS recently has been described as a highly specific hydrolase (a member of the HIT family) that catalyses the cleavage of m(7)GpppG and short capped oligoribonucleotides. We have demonstrated here that cap-1 (m(7)GpppGm) is a preferred substrate among several investigated dinucleotide cap analogues m(7)Gp(n)N (n = 3-5, N is a purine or pyrimidine base) and m(7)GMP is always one of the reaction product. Cap analogues containing pyrimidine base instead of guanine or diphosphate chain are resistant to hydrolysis catalyzed by human scavenger. Contrary to the other enzymes of HIT family, hDcpS activity is not stimulated by Mg(2+).
authors: Department of Biophysics, Institute of Experimental Physics, Warsaw University, 02-089 Warsaw, Poland.
authors: Zbigniew M
authors: ZM
authors: Darzynkiewicz
authors: Elzbieta
authors: E
authors: Bojarska
authors: Janusz
authors: J
authors: Stepinski
authors: Jacek
authors: J
authors: Jemielity
authors: Marzena
authors: M
authors: Jankowska-Anyszka
authors: Richard E
authors: RE
authors: Davis
authors: Edward
authors: E
authors: Darzynkiewicz
mesh_terms: D015226:Dinucleoside Phosphates / Q000737:chemistry*; D004722:Endoribonucleases / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D012314:RNA Cap Analogs / Q000737:chemistry*; D020871:RNA Stability
chemical_list: D015226:Dinucleoside Phosphates; D012314:RNA Cap Analogs; C062229:7-methyl-diguanosine triphosphate; D004722:Endoribonucleases; C485332:DcpS protein, human