title: Hypoxia and the presence of human vascular endothelial cells affect prostate cancer cell invasion and metabolism.
abstract: Tumor progression and metastasis are influenced by hypoxia, as well as by interactions between cancer cells and components of the stroma, such as endothelial cells. Here, we have used a magnetic resonance (MR)-compatible invasion assay to further understand the effects of hypoxia on human prostate cancer cell invasion and metabolism in the presence and absence of human umbilical vein endothelial cells (HUVECs). Additionally, we compared endogenous activities of selected proteases related to invasion in PC-3 cells and HUVECs, profiled gene expression of PC-3 cells by microarray, and evaluated cell proliferation of PC-3 cells and HUVECs by flow cytometry, under hypoxic and oxygenated conditions. The invasion of less-invasive DU-145 cells was not affected by either hypoxia or the presence of HUVECs. However, hypoxia significantly decreased the invasion of PC-3 cells. This hypoxia-induced decrease was attenuated by the presence of HUVECs, whereas under oxygenated conditions, HUVECs did not alter the invasion of PC-3 cells. Cell metabolism changed distinctly with hypoxia and invasion. The endogenous activity of selected extracellular proteases, although altered by hypoxia, did not fully explain the hypoxia-induced changes in invasion. Gene expression profiling indicated that hypoxia affects multiple cellular functions and pathways.
authors: Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, 720 Rutland Ave., Traylor Bldg. 217, Baltimore, MD 21205, USA.
authors: Ellen
authors: E
authors: Ackerstaff
authors: Dmitri
authors: D
authors: Artemov
authors: Robert J
authors: RJ
authors: Gillies
authors: Zaver M
authors: ZM
authors: Bhujwalla
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D002450:Cell Communication; D015687:Cell Hypoxia / Q000502:physiology; D045744:Cell Line, Tumor / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured / Q000378:metabolism; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000503:physiopathology*; D005109:Extracellular Matrix / Q000378:metabolism; D005782:Gels; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness / Q000503:physiopathology*; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D010100:Oxygen / Q000494:pharmacology; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics
chemical_list: D005782:Gels; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D010100:Oxygen