title: [Effect of human bone marrow mesenchymal stem cells on T lymphocyte killing K562 cells].
abstract: The objective of this study was to investigate the effect of human bone marrow mesenchymal stem cells (MSCs) on T lymphocyte killing K562 cells. MSCs were isolated from bone marrow and cultured, T cells were harvested by using nylon column method from peripheral blood. The T cells were co-cultured with MSCs, the phenotype expressions of T cell subsets were detected by flow cytometry. Killing effects of T cells (culture alone and co-culture with MSCs) on K562 cells were detected by LDH, expressions of IFN-gamma and IL-4 were detected by ELISA. The results showed that after T cells were co-cultured with MSCs for three days, the proportion of CD4+ and CD4+CD25+ T cells raised significantly (p<0.05) as compared with group of culture alone, but the proportion of CD8+ T cell were not significantly changed (p>0.05). In group of T cells co-cultured with MSCs, killing effects of T cells on K562 cells weakened, at the same time, expression of IFN-gamma decreased while expression of IL-4 increased. It is concluded that the MSCs weaken killing effects of T cells on K562 cells, which associates with increase of CD4+CD25+ T cell subsets and changes of IFN-gamma and IL-4 levels.
authors: Department of Hematology, General Hospital of PLA Lanzhou Military Area, Lanzhou 730050, China.
authors: Zhi-Gang
authors: ZG
authors: Si
authors: Hai
authors: H
authors: Bai
authors: Cun-Bang
authors: CB
authors: Wang
authors: Zhi-Wen
authors: ZW
authors: Xue
authors: Qian
authors: Q
authors: Wang
authors: Tao
authors: T
authors: Wu
authors: Xiao-Liang
authors: XL
authors: Yang
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology; D015704:CD4 Antigens / Q000276:immunology; D018920:Coculture Techniques; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D015847:Interleukin-4 / Q000378:metabolism; D020014:K562 Cells; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D015704:CD4 Antigens; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D015847:Interleukin-4; D007371:Interferon-gamma