[RNA interference inhibits the expression of survivin and induces the apoptosis of PC-3 cells].
OBJECTIVE
To investigate the inhibitory effect of RNA interference (RNAi) on the expression of survivin mRNA and its inducibility of the apoptosis of PC-3 cells.


METHODS
siRNA expression vectors were designed and constructed to be directed at survivin and transfected into PC-3 cells by Lipofectamine in 4 groups: plasmid A, plasmid B, negative sequence and control E. RT-PCR, Western blot and flow cytometry were used to detect the expression of survivin mRNA and the apoptosis of PC-3 cells.


RESULTS
The expression rates of survivin protein were 18.94% +/- 0.63%, 16.35 +/- 0.23%, 46.41% +/- 0.76% and 46.20 +/- 1.47%, those of survivin mRNA were 27.94% +/- 1.43%, 24.51% +/- 1.37%, 49.46% +/- 0.71% and 48.49% +/- 1.32%, and the apoptosis rates of PC-3 cells were 12.80% +/- 1.33%, 16.48% +/- 1.00%, 3.03% +/- 0.62% and 2.96% +/- 0.41% respectively in different groups.


CONCLUSIONS
RNAi can effectively inhibit the expression of survivin mRNA and induce the apoptosis of PC-3 cells.
Wang
Yi-bing
YB
Department of Urology, the Fourth Hospital Affiliated to China Medical University, Shenyang, Liaoning 110032, China.
Liu
Yi-li
YL
Wang
Ping
P
Fu
Xiao-liang
XL
Sun
Zhi-xi
ZX
D017209:Apoptosis; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008055:Lipids / Q000737:chemistry; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin; D014162:Transfection / Q000379:methods
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008055:Lipids; C086724:Lipofectamine; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000077022:Survivin