Hepatopancreatic multi-transcript expression patterns in the crayfish Cherax quadricarinatus during the moult cycle.
Alterations of hepatopancreatic multi-transcript expression patterns, related to induced moult cycle, were identified in male Cherax quadricarinatus through cDNA microarray hybridizations of hepatopancreatic transcript populations. Moult was induced by X-organ sinus gland extirpation or by repeated injections of 20-hydroxyecdysone. Manipulated males were sacrificed at premoult or early postmoult, and a reference population was sacrificed at intermoult. Differentially expressed genes among the four combinations of two induction methods and two moult stages were identified. Biologically interesting clusters revealing concurrently changing transcript expressions across treatments were selected, characterized by a general shift of expression throughout premoult and early postmoult vs. intermoult, or by different premoult vs. postmoult expressions. A number of genes were differentially expressed in 20-hydroxyecdysone-injected crayfish vs. X-organ sinus gland extirpated males.
Yudkovski
Y
Y
Israel Oceanographic and Limnological Research, Haifa, Israel.
Shechter
A
A
Chalifa-Caspi
V
V
Auslander
M
M
Ophir
R
R
Dauphin-Villemant
C
C
Waterman
M
M
Sagi
A
A
Tom
M
M
D000818:Animals; D003400:Astacoidea / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004441:Ecdysterone / Q000494:pharmacology; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D043143:Hepatopancreas / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D018978:Molting / Q000187:drug effects / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis
D017931:DNA Primers; D004441:Ecdysterone