title: Effect of dietary epigallocatechin-3-gallate on cytochrome P450 2E1-dependent alcoholic liver damage: enhancement of fatty acid oxidation.
abstract: This study was designed to determine whether dietary epigallocatechin-3-gallate (EGCG), the most abundant catechin polyphenol in green tea, can protect the liver from cytochrome P450 2E1 (CYP2E1)-dependent alcoholic liver damage. Compared with an ethanol group, when EGCG was present in the ethanol diet, the formation of a fatty liver was significantly reduced and the serum aspartate transaminase (AST) and alanine transaminase (ALT) levels were much lower. Ethanol treatment significantly elevated hepatic CYP2E1 expression while simultaneously reducing hepatic phospho-acetyl CoA carboxylase (p-ACC) and carnitine palmitoyl-transferase 1 (CPT-1) levels. While EGCG markedly reversed the effect of ethanol on hepatic p-ACC and CPT-1 levels, it had no effect on the ethanol-induced elevation in CYP2E1 expression. EGCG prevents ethanol-induced hepatotoxicity and inhibits the development of a fatty liver. These effects were associated with improvements in p-ACC and CPT-1 levels. The use of EGCG might be useful in treating patients with an alcoholic fatty liver.
authors: Amorepacific Corporation R&D Center, Yongin-si, Gyeonggi-do, Republic of Korea.
authors: Jun-Won
authors: JW
authors: Yun
authors: Young-Kyung
authors: YK
authors: Kim
authors: Byoung-Seok
authors: BS
authors: Lee
authors: Chae-Wook
authors: CW
authors: Kim
authors: Jin-Sook
authors: JS
authors: Hyun
authors: Joo-Hyun
authors: JH
authors: Baik
authors: Jung-Ju
authors: JJ
authors: Kim
authors: Bae-Hwan
authors: BH
authors: Kim
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D004789:Enzyme Activation; D005235:Fatty Liver, Alcoholic / Q000188:drug therapy* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D002392:Catechin; C045651:epigallocatechin gallate; D019392:Cytochrome P-450 CYP2E1; D002334:Carnitine O-Palmitoyltransferase; D001219:Aspartate Aminotransferases; D000103:Acetyl-CoA Carboxylase