In vitro cytotoxicity of dental alloys and cpTi obtained by casting.
BACKGROUND
This study aimed to compare the cytotoxicity of base-metal dental alloys and to evaluate if the casting method could influence their cytotoxicity.


METHODS
Disks of base-metal dental alloys were cast by two methods: plasma, under argon atmosphere, injected by vacuum-pressure; and oxygen-gas flame, injected by centrifugation, except Ti-6Al-4V and commercially pure titanium (cpTi), cast only by plasma. SCC9 cells were cultured in culture media D-MEM/Ham's F12 supplemented, at 37 degrees C in a humidified atmosphere of 5% carbon dioxide and 95% air, on the previously prepared disks. At subconfluence in wells without disks (control), cell number and viability were evaluated.


RESULTS
In plasma method, cpTi and Ti-6Al-4V were similar to control and presented higher number of cells than all other alloys, followed by Ni-Cr. In oxygen-gas flame method, all alloys presented fewer cells than control. Ni-Cr presented more cells than any other alloy, followed by Co-Cr-Mo-W which presented more cells than Ni-Cr-Ti, Co-Cr-Mo, and Ni-Cr-Be. There were no significant differences between casting methods related to cell number. Cell viability was not affected by either chemical composition or casting methods.


CONCLUSIONS
cpTi and Ti-6Al-4V were not cytotoxic while Ni-Cr-Be was the most cytotoxic among tested alloys. The casting method did not affect cytotoxicity of the alloys.
Faria
Adriana C L
AC
Department of Dental Materials and Prostheses, Dental School of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
Rosa
Adalberto L
AL
Rodrigues
Renata C S
RC
Ribeiro
Ricardo F
RF
D045744:Cell Line, Tumor; D002470:Cell Survival; D003343:Corrosion; D003722:Dental Alloys / Q000009:adverse effects*; D003735:Dental Casting Technique; D006801:Humans; D008422:Materials Testing / Q000379:methods
D003722:Dental Alloys