title: Evaluation of bone healing with eggshell-derived bone graft substitutes in rat calvaria: a pilot study.
abstract: The purpose of this study was to investigate the potential effectiveness of a surface-modified natural calcium carbonate, hen eggshell (ES) as a bone graft substitute. The surface characteristics, cell viability on, and osteoconductivity of, particulated ES with and without hydrothermal treatment in phosphate solutions were evaluated. Hydrothermal treatment partially converted ES to calcium-deficient hydroxyapatite (HA) with surface microstructure. MTT assay indicated higher osteoblast viability on surface-modified ES compared with a commercially available bone substitute, anorganic bovine bone (Bio-Oss, BO) (p < 0.001). Histological and histomorphometric analysis showed significantly greater new bone formation and mineralized bone-to-graft contact of surface-modified ES, especially with hydrothermally treated ES, compared with BO in 5-mm diameter calvarial defects in rats at 4 and 8 weeks of healing (p < 0.01). Complete bony bridging was more frequently found with hydrothermally treated ES. The results of this pilot study indicate the potential efficacy of surface-modified particulated hen eggshell as an osteoconductive bone substitute in a rat calvarial defect model.
authors: Department of Periodontology, School of Dentistry, Kyungpook National University, 188-1 Samduk 2Ga, Jung-Gu, Daegu 700-412, Korea. jinwoo@mail.knu.ac.kr
authors: Jin-Woo
authors: JW
authors: Park
authors: Sang-Ryul
authors: SR
authors: Bae
authors: Jo-Young
authors: JY
authors: Suh
authors: Dong-Hee
authors: DH
authors: Lee
authors: Sang-Hyun
authors: SH
authors: Kim
authors: Hyungjun
authors: H
authors: Kim
authors: Chong-Soo
authors: CS
authors: Lee
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000737:chemistry*; D016025:Bone Transplantation; D002460:Cell Line; D002470:Cell Survival; D002645:Chickens; D004528:Egg Shell / Q000737:chemistry*; D006358:Hot Temperature; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000737:chemistry; D010865:Pilot Projects; D011208:Powders / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000033:anatomy & histology / Q000637:transplantation*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D023822:Tissue Engineering; D014945:Wound Healing; D014961:X-Ray Diffraction
chemical_list: C077540:Bio-Oss; D018786:Bone Substitutes; D008903:Minerals; D011208:Powders