Determination of specific growth rate by measurement of specific rate of ribosome synthesis in growing and nongrowing cultures of Acinetobacter calcoaceticus.
RT-RiboSyn measures the specific rate of ribosome synthesis in distinct microbial populations by measuring the generation rate of precursor 16S rRNA relative to that of mature 16S rRNA when precursor 16S rRNA processing is inhibited. Good agreement was demonstrated between specific rate of ribosome synthesis and specific growth rate of Acinetobacter calcoaceticus.
Cutter
Matthew R
MR
Department of Civil and Environmental Engineering, University of South Florida, 4202 East Fowler Ave., ENB118, Tampa, FL 33620-5350, USA.
Stroot
Peter G
PG
D016954:Acinetobacter calcoaceticus / Q000254:growth & development* / Q000378:metabolism*; D003470:Culture Media; D008967:Molecular Biology / Q000295:instrumentation / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012270:Ribosomes / Q000378:metabolism*; D013053:Spectrophotometry / Q000379:methods
D003470:Culture Media; D012336:RNA, Ribosomal, 16S