[Spontaneous response and mechanism to ischemia in diabetic mouse].
OBJECTIVE
To investigate the spontaneous response and its mechanism of type 2 diabetes mouse to surgical induced hind limb ischemia.


METHODS
Forty mice were randomly divided into two groups, one was fed with normal chow, and another was fed with high fat diet. Twelve weeks later, mice were surgical induced to hind limb ischemia. Blood flow was monitored. Four weeks later, mice were sacrificed and muscle tissues were harvested for histological detection, Western Blot, ELISA and RT-PCR examination respectively.


RESULTS
Spontaneous restoration of blood flow was significantly slow down in type 2 diabetic mice with lower level of vascular density in ischemic muscles. VEGF signaling pathway was impaired although the VEGF levels were increased in diabetes.


CONCLUSIONS
The spontaneous response to ischemia was attenuated with an abnormal reaction of VEGF signaling pathway in the setting of type 2 diabetes mellitus.
Li
Yong-jun
YJ
Department of Vascular Surgery, Peking Union Medical College Hospital, and Chinese Academy of Medical Sciences, Beijing 100730, China. yongj_93@hotmail.com
Lin
Qing
Q
Liu
Chang-wei
CW
Guan
Heng
H
Liu
Xia-lin
XL
D000818:Animals; D015153:Blotting, Western; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000503:physiopathology*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D006614:Hindlimb / Q000098:blood supply / Q000378:metabolism; D007511:Ischemia / Q000209:etiology / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D011897:Random Allocation; D040262:Receptors, Vascular Endothelial Growth Factor / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D042461:Vascular Endothelial Growth Factor A; D040262:Receptors, Vascular Endothelial Growth Factor