Multipoint quantification of multimarker genes in peripheral blood and micrometastasis characteristic in peri-operative esophageal cancer patients.
This work proposed a method to assess the occult micrometastasis characteristic in peri-operative peripheral blood (PB) based on multipoint quantification of multimarker genes by real-time semiquantitative RT-PCR. The expression levels of SCC, CK19, CK20, CEA and survivin mRNA in PB samples collected before surgery (B-1), immediately after surgery (B0) and at the third day post-operatively (B+3) from 36 squamous esophageal cancer (EC) patients were detected. SCC and CK19 mRNA showed low positivity detection rates, while the rate for CEA and survivin mRNA panel was 58.3%, 83.3% and 72.3% at B-1, B0 and B+3, respectively. Opposite to the significant increase and slow decrease of CEA cells at stages of B-1 to B0 and B0 to B+3, respectively, survivin cells decreased significantly and increased quickly at the two stages. The follow-up with a period of 1.2 years showed that the patients with the B+3/B0 ratios of >or= 0.3 for CEA cells or/and >or= 10 for survivin cells exhibited significantly high possibility of developed metastasis, and the sensitivity to predict developed metastasis increased from 54.5% of CEA mRNA alone to 72.7% of CEA and survivin mRNA panel. These results suggested that CEA and survivin gene panel improved the sensitivity to predict recurrence and differentiate the micrometastatic process.
Liu
Zhian
Z
MOE Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093, China.
Jiang
Ming
M
Yan
Feng
F
Xu
Lin
L
Zhao
Jianhua
J
Ju
Huangxian
H
D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D005260:Female; D052138:Genes, Neoplasm; D006801:Humans; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000235:genetics*; D009364:Neoplasm Recurrence, Local; D019990:Perioperative Care; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D014408:Biomarkers, Tumor