title: Quantitative telomeric overhang determination using a double-strand specific nuclease.
abstract: Telomeres terminate in 3' overhangs that function in end protection and the formation of t-loops. Determining the steps and factors involved in overhang processing is compromised by the inability to easily and accurately determine overhang size in the presence of many kilobases of double-stranded telomeric DNA. We here describe the use of a double-strand specific nuclease (DSN) that entirely digests double-stranded DNA including telomeres, leaving the overhangs intact so that they can be measured.
authors: Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors: Yong
authors: Y
authors: Zhao
authors: Hirotoshi
authors: H
authors: Hoshiyama
authors: Jerry W
authors: JW
authors: Shay
authors: Woodring E
authors: WE
authors: Wright
mesh_terms: D015139:Blotting, Southern; D004247:DNA / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D016615:Telomere / Q000737:chemistry* / Q000378:metabolism
chemical_list: D004247:DNA; D004706:Endodeoxyribonucleases