title: Candida rugosa lipase-catalysed kinetic resolution of 2-substituted-aryloxyacetic esters with dimethylsulfoxide and isopropanol as additives.
abstract: Candida rugosa lipase-catalysed hydrolysis of three different 2-substituted-aryloxyacetic esters was performed in aqueous buffer containing dimethyl sulphoxide and isopropanol from 0 to 80% v/v as additives, in order to obtain an enhancement of the enantioselectivity. For 2-(p-chlorophenoxy)acetic acid and 2-n-butyl-2-(p-chlorophenoxy)acetic acid ethyl esters, DMSO enhanced enzyme enantioselectivity more than IPA with an opposite enzymatic enantiopreference. The cosolvents moderately improved Candida rugosa lipase enantioselectivity for 2-phenyl-2-(p-chlorophenoxy)acetic acid ethyl ester.
authors: Department of Drug Sciences, University of Chieti G. d'Annunzio, via dei Vestini, Chieti, Italy.
authors: Alessandra
authors: A
authors: Ammazzalorso
authors: Rosa
authors: R
authors: Amoroso
authors: Giancarlo
authors: G
authors: Bettoni
authors: Barbara
authors: B
authors: De Filippis
authors: Marialuigia
authors: M
authors: Fantacuzzi
authors: Letizia
authors: L
authors: Giampietro
authors: Cristina
authors: C
authors: Maccallini
authors: Maria Luisa
authors: ML
authors: Tricca
mesh_terms: D019840:2-Propanol / Q000494:pharmacology*; D002175:Candida / Q000201:enzymology*; D002384:Catalysis / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004952:Esters / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D013237:Stereoisomerism
chemical_list: D004952:Esters; D008049:Lipase; D019840:2-Propanol; D004121:Dimethyl Sulfoxide