UVB-radiation-induced apoptosis in Jurkat cells: a coordinated fourier transform infrared spectroscopy-flow cytometry study.
We studied the induction of apoptosis in Jurkat cells by UVB radiation (wavelength 290-320 nm) at a dose of 310 mJ/ cm2. We combined Fourier transform infrared (FTIR) spectroscopy with flow cytometry to determine whether the combination of both techniques could provide new and improved information about cell modifications. To do this, we looked for correspondences and correlations between spectroscopy and flow cytometry data and found three highly probable spectroscopic markers of apoptosis. The behavior of the wave number shift of both the Amide I beta-sheet component and the area of the 1083 cm(-1) band reproduced, with a high correlation, the behavior of the early apoptotic cell population, while the behavior of the Amide I area showed a high correlation with the early plus late apoptotic cell population.
Pozzi
Deleana
D
Dipartimento di Medicina Sperimentale e Patologia, Università di Roma La Sapienza, Roma, Italy.
Grimaldi
Paola
P
Gaudenzi
Silvia
S
Di Giambattista
Lucia
L
Silvestri
Ida
I
Morrone
Stefania
S
Congiu Castellano
Agostina
A
D000577:Amides / Q000737:chemistry; D017209:Apoptosis / Q000528:radiation effects*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D019169:Jurkat Cells; D050356:Lipid Metabolism / Q000528:radiation effects; D009696:Nucleic Acids / Q000737:chemistry; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D014466:Ultraviolet Rays
D000577:Amides; D009696:Nucleic Acids; D011506:Proteins