title: Expression, purification and crystallization of human 5-lipoxygenase-activating protein with leukotriene-biosynthesis inhibitors.
abstract: The nuclear membrane protein 5-lipoxygenase-activating protein (FLAP) plays an essential role in leukotriene synthesis. Recombinant full-length human FLAP with a C-terminal hexahistidine tag has been expressed and purified from the cytoplasmic membrane of Escherichia coli. Diffraction-quality crystals of FLAP in complex with leukotriene-synthesis inhibitor MK-591 and with an iodinated analogue of MK-591 have been grown using the sitting-drop vapor-diffusion method. The crystals exhibit tetragonal symmetry (P42(1)2) and diffracted to a resolution limit of 4 A.
authors: Department of Medicinal Chemistry, Merck Research Laboratories, Rahway, NJ 07065, USA. shihua_xu@merck.com
authors: Shihua
authors: S
authors: Xu
authors: Brian M
authors: BM
authors: McKeever
authors: Douglas
authors: D
authors: Wisniewski
authors: Douglas K
authors: DK
authors: Miller
authors: Robert H
authors: RH
authors: Spencer
authors: Lin
authors: L
authors: Chu
authors: Feroze
authors: F
authors: Ujjainwalla
authors: Ting Ting
authors: TT
authors: Yamin
authors: Jilly F
authors: JF
authors: Evans
authors: Joseph W
authors: JW
authors: Becker
authors: Andrew D
authors: AD
authors: Ferguson
mesh_terms: D058927:5-Lipoxygenase-Activating Proteins; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D006801:Humans; D015289:Leukotrienes / Q000096:biosynthesis*; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015394:Molecular Structure
chemical_list: D058927:5-Lipoxygenase-Activating Proteins; C548592:ALOX5AP protein, human; D002352:Carrier Proteins; D015289:Leukotrienes; D008565:Membrane Proteins