[Antiviral action of some antioxidants/antihypoxants and their combinations with remantadine against human influenza A(H3N2) virus studied in in vitro models].
The possible antiviral activity of preparations with antioxidant and/or antihypoxant properties was studied on two in vitro models of influenza infection: (i) in cultures of chorio-allantoic membranes of chicken embryos and (ii) in MDCK cells. Preparations under study were hypoxene, reduced glutathione, dihydroquercetin, trolox, coenzyme Q10, and the enzymatic preparation of superoxide-dismutase (recsod). Preparations possessing combined antioxidant/antihypoxic and detoxicating properties (reduced glutathione and hypoxene) produced a significant antiviral effect and enhanced the antiviral effect of rimantadine. The antiviral effect of these preparations was manifested by a decrease in the production of viral particles and, to a more pronounced degree, by the inhibition of cytopathogenic action of virus on cultured cells, which was revealed in the tests for the activity of respiratory enzymes. In contrast to the compounds containing thio or sulfo groups, the antioxidants of "direct action" (free radical scavengers) - coenzyme Q 10, trolox, quercetin and the enzymatic preparation recsod did not show any pronounced protective effect and in some cases even enhanced the production of viral particles and decreased the antiviral action of rimantadine.
Eropkin
M Iu
MIu
Gudkova
T M
TM
Konovalova
N I
NI
Shchekanova
S M
SM
Iaglovskaia
I B
IB
Eropkina
E M
EM
Kiselev
O I
OI
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000821:virology; D004285:Dogs; D004336:Drug Antagonism; D004357:Drug Synergism; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D053122:Influenza A Virus, H3N2 Subtype / Q000187:drug effects*; D010647:Phenyl Ethers / Q000494:pharmacology; D012299:Rimantadine / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014771:Virion / Q000187:drug effects; D014773:Virology / Q000379:methods
D000975:Antioxidants; D000998:Antiviral Agents; D016166:Free Radical Scavengers; D010647:Phenyl Ethers; D012299:Rimantadine; C085806:olifen; D005978:Glutathione