title: Distribution of ochratoxin A in plasma and tissues of rats fed a naturally contaminated diet amended with micronized wheat fibres: effectiveness of mycotoxin sequestering activity.
abstract: The effectiveness of micronized wheat fibres (MWF) alone or in association with yeast cell walls (YCW) as active adsorbents to decrease, in vivo, the levels of ochratoxin A (OTA) was checked in a total of 48 rats, equitably distributed into four groups: (1) control; (2) OTA naturally contaminated diet (2.2 microg/g); (3) OTA naturally contaminated diet (2.2 microg/g) amended with MWF (2%); (4) OTA naturally contaminated diet (2 microg/g) amended with MWF (1.8%) in association with YCW (0.2%). A 4 week experimental period corresponding to a daily intake in the range of 132.2-146.1 microg OTA/kg bw decreased the rat body weight gains, as compared to the controls. The adsorbents did not significantly alleviate the growth depression caused by the contaminated diet. However, a significant protective effect of MWF was observed in terms of OTA concentration in plasma (40.5% decrease), kidney (28.1% decrease) and liver (38.8% decrease). Mixing this sorbent with the YCW did not significantly improve its protective activity against OTA. The faecal OTA concentrations were higher for the MWF and MWF+YCW treated animals, as compared to the positive control (group II). Taken together, these results suggest that MWF are a promising tool to counteract the toxic effects of OTA naturally contaminated diets.
authors: Unité de Biochimie de la Nutrition, Faculté d'Ingénierie Biologique, Agronomique et Environnementale, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
authors: N
authors: N
authors: Aoudia
authors: E K
authors: EK
authors: Tangni
authors: Y
authors: Y
authors: Larondelle
mesh_terms: D000818:Animals; D001835:Body Weight; D002846:Chromatography, Affinity; D004032:Diet; D005243:Feces; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009793:Ochratoxins / Q000097:blood / Q000493:pharmacokinetics*; D009929:Organ Size; D051381:Rats; D014018:Tissue Distribution; D014908:Triticum
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A