title: Developmental validation of reduced-size STR Miniplex primer sets.
abstract: This paper describes a developmental validation study of three Miniplex sets covering 12 of the 13 CODIS loci. As these new sets will be used for the analysis of degraded and low level DNA, the validation studies were performed using 100-125 pg of DNA, the lowest input level at which peak balance, peak intensity, and allele consistency were stable. To demonstrate the applicability of the Miniplex sets to forensic casework, these validation studies were completed in accordance with the Scientific Working Group on DNA Analysis Methods (SWGDAM). A range of tests were performed including studies of concordance with standard multiplex kits, sensitivity and reproducibility, and PCR amplification conditions. Additionally, studies of mixtures, nonhuman and environmentally degraded DNA, and simulated forensic samples were performed. Our results demonstrate that Miniplex STR amplification procedures are a robust and sensitive tool for the analysis of degraded DNA.
authors: International Forensic Research Institute, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA.
authors: Kerry L
authors: KL
authors: Opel
authors: Denise T
authors: DT
authors: Chung
authors: Jirí
authors: J
authors: Drábek
authors: John M
authors: JM
authors: Butler
authors: Bruce R
authors: BR
authors: McCord
mesh_terms: D001801:Blood Stains; D004247:DNA / Q000032:analysis*; D053836:DNA Degradation, Necrotic; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D020080:Tandem Repeat Sequences
chemical_list: D017931:DNA Primers; D004247:DNA