Tandem mass spectrometry in physiology.
Tandem mass spectrometry coupled to liquid chromatography (LC-MS/MS) allows identification of proteins in a complex mixture without need for protein purification ("shotgun" proteomics). Recent progress in LC-MS/MS-based quantification, phosphoproteomic analysis, and targeted LC-MS/MS using multiple reaction monitoring (MRM) has made LC-MS/MS a powerful tool for the study of cell physiology.
Pisitkun
Trairak
T
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.
Hoffert
Jason D
JD
Yu
Ming-Jiun
MJ
Knepper
Mark A
MA
D000818:Animals; D002468:Cell Physiological Phenomena; D002853:Chromatography, Liquid; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis*; D010750:Phosphoproteins / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction; D018511:Systems Integration; D053719:Tandem Mass Spectrometry
D047908:Intracellular Signaling Peptides and Proteins; D010750:Phosphoproteins