Applications of a microplate reader in yeast physiology research.
Microplate readers have been useful assistants of researchers for several decades. This work is focused on the applications of a simple absorbance microplate reader in yeast physiology research, and its advantages and limitations in comparison with alternative methods are discussed. The two main procedures involved are measuring growth curves and monitoring the pH changes of medium using two different pH indicators. We suggest mathematical formulas for converting absorbance data into pH values. With a microplate reader as many as 96 samples can be simultaneously analyzed, while medium consumption is minimized to 100 microL per sample. The results can be observed in 24-48 h (for growth curves) or in 1-3 h (for pH changes) with minimal hands-on time required.
Maresová
Lydie
L
Department of Membrane Transport, Institute of Physiology, Academy of Sciences of the Czech Republic, 142-20 Prague 4, Czech Republic. lydie@biomed.cas.cz
Sychrová
Hana
H
D000143:Acids / Q000378:metabolism; D001961:Bromcresol Green / Q000378:metabolism; D001962:Bromcresol Purple / Q000378:metabolism; D002138:Calibration; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D010783:Photometry / Q000295:instrumentation* / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000254:growth & development / Q000502:physiology*
D000143:Acids; D003470:Culture Media; D001962:Bromcresol Purple; D001961:Bromcresol Green