Downregulation of myc promoter-binding protein 1 (MBP-1) in growth-arrested malignant B cells.
Normal human B lymphocytes are sensitive to the growth-inhibitory action of TGF-beta1 whereas malignant B lymphoma cells are mostly resistant to TGF-beta1 effects. We have shown in our previous work that, TGF-beta1 treatment resulted in significant growth inhibition of the DoHH2 cell line. In the present study we showed that TGF-beta1-induced growth arrest was associated with notable downregulation of the myc-binding protein-1 (MBP-1). Moreover, our results indicated that c-Myc overexpression in TGF-beta1-arrested malignant B cells is mediated by binding of MBP-1, as a transcription repressor, to the (+118/+153) element of the promoter region of the myc gene.
Tvrdík
D
D
Institute of Pathology, Charles University in Prague, 1st Faculty of Medicine and General Teaching Hospital, Prague, Czech Republic. Daniel.Tvrdik@vfn.cz
Dundr
P
P
Povýsil
C
C
Prikryl
P
P
Melcáková
S
S
Planková
M
M
D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D011401:Promoter Regions, Genetic; D011485:Protein Binding / Q000187:drug effects; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
C088830:DENND4A protein, human; D015342:DNA Probes; D004268:DNA-Binding Proteins; D016271:Proto-Oncogene Proteins c-myc; D053773:Transforming Growth Factor beta1