Comparison between chicken and rabbit antibody based particle enhanced cystatin C reagents for immunoturbidimetry.
We have compared three commercial particle enhanced cystatin C reagents. One of the reagents utilizes chicken antibodies and the other two reagents are rabbit antibody based. We show that the chicken antibody based reagent yields a higher delta absorbance when reacting with the antigen. IgY coupled to latex particles show a strong scatter response even at high antigen concentrations in contrast to the steep decline in scatter previously reported for IgY antibodies in solution. The reagent also showed a low CV for duplicate samples. Laying hens thus seems as an interesting source of antibodies for particle-enhanced immunoassays.
Hansson
Lars-Olof
LO
Department of Medical Sciences, University Hospital, Uppsala, Sweden.
Flodin
Mats
M
Nilsen
Tom
T
Caldwell
Karin
K
Fromell
Karin
K
Sunde
Kathrin
K
Larsson
Anders
A
D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D002645:Chickens / Q000276:immunology; D055316:Cystatin C; D015891:Cystatins / Q000097:blood* / Q000276:immunology; D007118:Immunoassay; D007136:Immunoglobulins / Q000737:chemistry / Q000276:immunology*; D007202:Indicators and Reagents; D008027:Light; D008863:Microspheres; D009391:Nephelometry and Turbidimetry; D011817:Rabbits; D012542:Scattering, Radiation
D000906:Antibodies; D000941:Antigens; D055316:Cystatin C; D015891:Cystatins; C016512:IgY; D007136:Immunoglobulins; D007202:Indicators and Reagents