Acceleration of bone formation with BMP2 in frame-reinforced carbonate apatite-collagen sponge scaffolds.
The development is expected of scaffold biomaterials that feature a shape-maintaining property in addition to high porosity and large pores that cells can easily invade. To develop a new biodegradable scaffold biomaterial reinforced with a frame, synthesized carbonate apatite (CO3Ap) was mixed with neutralized collagen gel, and the CO3Ap-collagen mixtures were lyophilized into sponges in a porous hydroxyapatite (HAp) frame ring. X-ray diffraction and Fourier transform infrared spectroscopy (FT-IR) analyses together with chemical analysis indicated that the synthesized CO3Ap had a crystalline nature and a chemical composition similar to that of bone. Scanning electron microscope (SEM) observation showed that the CO3Ap-collagen sponge had a sui pore size for cell invasion. In proliferation and differentiation experiments with osteoblasts, alkaline phosphatase and osteopontin activity were clearly detected. When these sponge-frame complexes with bone morphogenic protein (rh-BMP2) were implanted beneath the periosteum cranii of rats, significant new bone was created at the surface of the periosteum cranii after 4 weeks of implantation. These reinforced CO3Ap-collagen sponges with rh-BMP2 are expected to be used as hard tissue scaffold biomaterials for the therapeutic purpose of the rapid cure of bone defects.
Hirata
Isao
I
Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
Nomura
Yuji
Y
Ito
Manabu
M
Shimazu
Atsushi
A
Okazaki
Masayuki
M
D020341:Absorbable Implants; D000818:Animals; D001031:Apatites / Q000737:chemistry*; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002254:Carbonates / Q000737:chemistry*; D002417:Cattle; D018929:Cell Culture Techniques; D003094:Collagen / Q000737:chemistry*; D005782:Gels; D006801:Humans; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D016062:Porosity; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014961:X-Ray Diffraction
D001031:Apatites; C525718:BMP2 protein, human; C525719:Bmp2 protein, mouse; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D002254:Carbonates; D005782:Gels; D016212:Transforming Growth Factor beta; D003094:Collagen