Arabidopsis P-glycoprotein19 participates in the inhibition of gravitropism by gravacin.
ATP-binding cassette (ABC) transporters have been implicated in a multitude of biological pathways. In plants, some ABC transporters are involved in the polar transport of the plant hormone auxin and the gravitropic response. We previously identified Gravacin as a potent inhibitor of gravitropism in Arabidopsis thaliana. We demonstrate that P-glycoprotein19 (PGP19) is a target for Gravacin and participates in its inhibition of gravitropism. Gravacin inhibited the auxin transport activity of PGP19 and PGP19-PIN complexes. Furthermore, we identified E1174 as an important residue for PGP19 activity and its ability to form active transport complexes with PIN1. Gravacin is an auxin transport inhibitor that inhibits PGPs, particularly PGP19, which can be used to further dissect the role of PGP19 without the inhibition of other auxin transporters, namely PIN proteins.
Rojas-Pierce
Marcela
M
Department of Botany and Plant Sciences, University of California, Riverside, Riverside, CA 92521, USA.
Titapiwatanakun
Boosaree
B
Sohn
Eun Ju
EJ
Fang
Fang
F
Larive
Cynthia K
CK
Blakeslee
Joshua
J
Cheng
Yan
Y
Cutler
Sean R
SR
Cuttler
Sean
S
Peer
Wendy A
WA
Murphy
Angus S
AS
Raikhel
Natasha V
NV
D018528:ATP-Binding Cassette Transporters / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D020346:Aquaporins / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D001667:Binding, Competitive / Q000502:physiology; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D005663:Furans / Q000378:metabolism / Q000494:pharmacology*; D018522:Gravitropism / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D018546:Hypocotyl / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D015394:Molecular Structure; D009154:Mutation; D010797:Phthalimides / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
D018528:ATP-Binding Cassette Transporters; D020346:Aquaporins; D029681:Arabidopsis Proteins; D005663:Furans; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; D010797:Phthalimides; D011994:Recombinant Proteins; C114215:ATPGP1 protein, Arabidopsis; C527265:Gravacin; C519873:PGP19 protein, Arabidopsis; C116363:PIN1 protein, Arabidopsis; C117013:AT5G57090 protein, Arabidopsis; C099402:delta-TIP protein, Arabidopsis; C002687:alpha-naphthylphthalamic acid