title: Applications of mesenchymal stem cells labeled with Tat peptide conjugated quantum dots to cell tracking in mouse body.
abstract: Fluorescent quantum dots have great potential in cellular labeling and tracking. Here, PEG encapsulated CdSe/ZnS quantum dots have been conjugated with Tat peptide, and introduced into living mesenchymal stem cells. The Tat peptide conjugated quantum dots in mesenchymal stem cells were assessed by fluorescent microscopy, laser confocal microscope and. flow cytometry. The result shows that Tat peptide conjugated quantum dots could enter mesenchymal stem cells efficiently. The Tat-quantum dots labeled stem cells were further injected into the tail veins of NOD/SCID beta2 M null mice, and the tissue distribution of these labeled cells in nude mice were examined with fluorescence microscope. The result shows that characteristic fluorescence of quantum dots was observed primarily in the liver, the lung and the spleen, with little or no quantum dots accumulation in the brain, the heart, or the kidney.
authors: Micro-Nano Technologies Research Center and State Key Lab of CBSC, Hunan University, Changsha 410082, China.
authors: Yun
authors: Y
authors: Lei
authors: Haiyang
authors: H
authors: Tang
authors: Lide
authors: L
authors: Yao
authors: Richeng
authors: R
authors: Yu
authors: Meifu
authors: M
authors: Feng
authors: Bingsuo
authors: B
authors: Zou
mesh_terms: D000818:Animals; D015696:Gene Products, tat / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D045663:Quantum Dots
chemical_list: D015696:Gene Products, tat