title: Molecular characterization of the first polyomavirus from a New World primate: squirrel monkey polyomavirus.
abstract: DNA samples from a variety of New World monkeys were screened by using a broad-spectrum PCR targeting the VP1 gene of polyomaviruses. This resulted in the characterization of the first polyomavirus from a New World primate. This virus naturally infects squirrel monkeys (Saimiri sp.) and is provisionally named squirrel monkey polyomavirus (SquiPyV). The complete genome of SquiPyV is 5,075 bp in length, and encodes the small T and large T antigens and the three structural proteins VP1, VP2 and VP3. Interestingly, the late region also encodes a putative agnoprotein, a feature that it shares with other polyomaviruses from humans, baboons and African green monkeys. Comparison with other polyomaviruses revealed limited sequence similarity to any other polyomavirus, and phylogenetic analysis of the VP1 gene confirmed its uniqueness.
authors: Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
authors: Ernst J
authors: EJ
authors: Verschoor
authors: Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
authors: Marlous J
authors: MJ
authors: Groenewoud
authors: Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
authors: Zahra
authors: Z
authors: Fagrouch
authors: Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
authors: Aruna
authors: A
authors: Kewalapat
authors: Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
authors: Sabine
authors: S
authors: van Gessel
authors: Department of Pathobiology, Pathology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors: Marja J L
authors: MJL
authors: Kik
authors: Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
authors: Jonathan L
authors: JL
authors: Heeney
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005784:Gene Amplification; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D011120:Polyomavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012453:Saimiri / Q000821:virology*
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral