title: SUMOylation of pontin chromatin-remodeling complex reveals a signal integration code in prostate cancer cells.
abstract: Posttranslational modification by small ubiquitin-like modifier (SUMO) controls diverse cellular functions of transcription factors and coregulators and participates in various cellular processes including signal transduction and transcriptional regulation. Here, we report that pontin, a component of chromatin-remodeling complexes, is SUMO-modified, and that SUMOylation of pontin is an active control mechanism for the transcriptional regulation of pontin on androgen-receptor target genes in prostate cancer cells. Biochemical purification of pontin-containing complexes revealed the presence of the Ubc9 SUMO-conjugating enzyme that underlies its function as an activator. Intriguingly, 5alpha-dihydroxytestosterone treatments significantly increased the SUMOylation of pontin, and SUMOylated pontin showed further activation of a subset of nuclear receptor-dependent transcription and led to an increase in proliferation and growth of prostate cancer cells. These data clearly define a functional model and provide a link between SUMO modification and prostate cancer progression.
authors: Department of Biological Sciences, Research Center for Functional Cellulomics and School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, South Korea.
authors: Jung Hwa
authors: JH
authors: Kim
authors: Ji Min
authors: JM
authors: Lee
authors: Hye Jin
authors: HJ
authors: Nam
authors: Hee June
authors: HJ
authors: Choi
authors: Jung Woo
authors: JW
authors: Yang
authors: Jason S
authors: JS
authors: Lee
authors: Mi Hyang
authors: MH
authors: Kim
authors: Su-Il
authors: SI
authors: Kim
authors: Chin Ha
authors: CH
authors: Chung
authors: Keun Il
authors: KI
authors: Kim
authors: Sung Hee
authors: SH
authors: Baek
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D002352:Carrier Proteins / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D002843:Chromatin / Q000737:chemistry*; D004265:DNA Helicases / Q000737:chemistry*; D006801:Humans; D006915:Hydroxytestosterones / Q000494:pharmacology; D008239:Lysine / Q000737:chemistry; D008297:Male; D008954:Models, Biological; D011471:Prostatic Neoplasms / Q000378:metabolism*; D025842:SUMO-1 Protein; D015398:Signal Transduction; D025841:Small Ubiquitin-Related Modifier Proteins / Q000737:chemistry*; D014158:Transcription, Genetic; D044763:Ubiquitin-Conjugating Enzymes / Q000378:metabolism; D000098843:Ubiquitin-Conjugating Enzyme UBC9
chemical_list: D002352:Carrier Proteins; D002843:Chromatin; D006915:Hydroxytestosterones; D025842:SUMO-1 Protein; C495501:SUMO1 protein, human; D025841:Small Ubiquitin-Related Modifier Proteins; D044763:Ubiquitin-Conjugating Enzymes; D000074183:ATPases Associated with Diverse Cellular Activities; D004265:DNA Helicases; C486549:RUVBL1 protein, human; D000098843:Ubiquitin-Conjugating Enzyme UBC9; D008239:Lysine