title: Juvenile hormone analogs do not affect directly the activity of the ecdysteroid receptor complex in insect culture cell lines.
abstract: During insect development, ecdysteroids and juvenile hormones (JHs) interact to regulate larval growth, metamorphosis and reproduction but the molecular mechanisms by which both hormones influence each other's activity remain unknown. Because of their ease of use and straightforward genetic manipulation, insect cell lines often have been used to clarify the actions and interactions of hormones at the molecular level. Here we report on the use of two insect culture cell lines, Drosophila melanogaster S2 and Bombyx mori Bm5 cells, to investigate two molecular processes in which ecdysteroids and JH have been shown to interact: (1) direct modulation of the activity of the ecdysteroid receptor transcription complex and (2) interference at the level of induction of the primary gene E75. Our data do not support JH analogs (JHAs) acting through the above processes: 'antagonism' of ecdysteroid receptor activity by JHAs correlated with cytotoxicity and induction of E75 expression by JHAs was not demonstrated. However, we confirm previous studies in which it was observed that methoprene can partially reverse the growth inhibition by 20E in S2 cells (but not Bm5 cells). Therefore, the molecular mechanism by which both hormones influence each other's activity to regulate cell growth in S2 cells remains unknown.
authors: Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, Belgium. thomas.soin@ugent.be
authors: Thomas
authors: T
authors: Soin
authors: Luc
authors: L
authors: Swevers
authors: Hadi
authors: H
authors: Mosallanejad
authors: Rodica
authors: R
authors: Efrose
authors: Vassiliki
authors: V
authors: Labropoulou
authors: Kostas
authors: K
authors: Iatrou
authors: Guy
authors: G
authors: Smagghe
mesh_terms: D000818:Animals; D012831:Bombyx / Q000166:cytology* / Q000187:drug effects*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D007306:Insecticides / Q000494:pharmacology; D007605:Juvenile Hormones / Q000819:agonists* / Q000037:antagonists & inhibitors*; D008726:Methoprene / Q000494:pharmacology; D048448:Phenylcarbamates / Q000494:pharmacology; D020033:Protein Isoforms; D011987:Receptors, Steroid / Q000737:chemistry / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D007306:Insecticides; D007605:Juvenile Hormones; D048448:Phenylcarbamates; D020033:Protein Isoforms; D011987:Receptors, Steroid; D014157:Transcription Factors; C066030:USP protein, Drosophila; C034808:ecdysteroid receptor; D008726:Methoprene; C052034:fenoxycarb