Limitation of usage of PicoGreen dye in quantitative assays of double-stranded DNA in the presence of intercalating compounds.
PicoGreen is a very sensitive fluorescent dye for quantitative assays of double-stranded DNA (dsDNA) in solution and is used in several analytical protocols in which sensitive and precise DNA detection is needed, also for examination of drug-DNA interactions. The data shown in this paper indicate that compounds intercalating to DNA influence the applicability of PicoGreen dye for quantitative measurements of dsDNA, and for this reason PicoGreen dye is not suitable for examination of drug-DNA interactions, especially interstrand DNA crosslinks.
Koba
Marcin
M
Department of Pharmaceutical Technology and Biochemistry, Gdańsk University of Technology, Gdańsk, Poland. kobamar@wp.pl
Szostek
Alicja
A
Konopa
Jerzy
J
D004247:DNA / Q000032:analysis*; D005470:Fluorometry; D018922:HL-60 Cells; D006801:Humans; D007364:Intercalating Agents / Q000737:chemistry*; D009930:Organic Chemicals / Q000737:chemistry; D012680:Sensitivity and Specificity
D007364:Intercalating Agents; D009930:Organic Chemicals; C099476:PicoGreen; D004247:DNA