Radiolabeling procedure, quality control and stability of 99mTc-labeled chondroitin sulfate: a new approach of targeting osteoarthritis.
Chondroitin sulfate (CS) is an endogenous component of cartilage which could determine osteoarthritis after radiolabeling. Radiolabeling was performed by (99m)TcO(4)(-)/tin method. We found that pH is a limiting factor. At pH 6.2 in 0.05 M Na acetate buffer 32.8% colloid was formed, at pH 5.0 in 0.5M Na acetate, in our methodology only 4.7% colloid. The tracer was highly stable. We conclude that (99m)TcCS could be a promising imaging agent of osteoarthritis due to simple radiolabeling and high stability.
Sobal
Grazyna
G
Department of Nuclear Medicine, Medical University of Vienna, A-1090 Vienna, Währinger Gürtel 18-20, Austria. Grazyna.Sobal@meduniwien.ac.at
Menzel
Ernst Johannes
EJ
Sinzinger
Helmut
H
D002809:Chondroitin Sulfates / Q000737:chemistry*; D002850:Chromatography, Gel; D004355:Drug Stability; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D020370:Osteoarthritis, Knee / Q000000981:diagnostic imaging*; D011786:Quality Control; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000737:chemistry*; D013667:Technetium / Q000737:chemistry*
D019275:Radiopharmaceuticals; D013667:Technetium; D002809:Chondroitin Sulfates