Yeast antizyme mediates degradation of yeast ornithine decarboxylase by yeast but not by mammalian proteasome: new insights on yeast antizyme.
Mammalian antizyme (mAz) is a central element of a feedback circuit regulating cellular polyamines by accelerating ornithine decarboxylase (ODC) degradation and inhibiting polyamine uptake. Although yeast antizyme (yAz) stimulates the degradation of yeast ODC (yODC), we show here that it has only a minor effect on polyamine uptake by yeast cells. A segment of yODC that parallels the Az binding segment of mammalian ODC (mODC) is required for its binding to yAz. Although demonstrating minimal homology to mAz, our results suggest that yAz stimulates yODC degradation via a similar mechanism of action. We demonstrate that interaction with yAz provokes degradation of yODC by yeast but not by mammalian proteasomes. This differential recognition may serve as a tool for investigating proteasome functions.
Porat
Ziv
Z
Department of Molecular Genetics, The Weizmann Institute of Science, 1 Hertzel St., Rehovot 76100, Israel.
Landau
Guy
G
Bercovich
Zippi
Z
Krutauz
Dasha
D
Glickman
Michael
M
Kahana
Chaim
C
D002460:Cell Line; D006801:Humans; D009955:Ornithine Decarboxylase / Q000235:genetics / Q000378:metabolism*; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity
C497099:Oaz1 protein, S cerevisiae; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; C031017:ornithine decarboxylase antizyme; D046988:Proteasome Endopeptidase Complex; D009955:Ornithine Decarboxylase