Optimization of antibacterial activity by Gold-Thread (Coptidis Rhizoma Franch) against Streptococcus mutans using evolutionary operation-factorial design technique.
This study was conducted to find the optimum extraction condition of Gold-Thread for antibacterial activity against Streptococcus mutans using The evolutionary operation-factorial design technique. Higher antibacterial activity was achieved in a higher extraction temperature (R2 = -0.79) and in a longer extraction time (R2 = -0.71). Antibacterial activity was not affected by differentiation of the ethanol concentration in the extraction solvent (R2 = -0.12). The maximum antibacterial activity of clove against S. mutans determined by the EVOP-factorial technique was obtained at 80 degrees C extraction temperature, 26 h extraction time, and 50% ethanol concentration. The population of S. mutans decreased from 6.110 logCFU/ml in the initial set to 4.125 logCFU/ml in the third set.
Choi
Ung-Kyu
UK
Department of Oriental Medicinal Food and Nutrition, Asia University, Gyeongsan 712-220, Korea.
Kim
Mi-Hyang
MH
Lee
Nan-Hee
NH
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000089042:Coptis chinensis; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D004365:Drugs, Chinese Herbal; C107393:Coptidis rhizoma extract