title: Validated reversed phase LC method for quantitative analysis of polymethoxyflavones in citrus peel extracts.
abstract: Polymethoxyflavones (PMFs), which exist exclusively in the citrus genus, have biological activities including anti-inflammatory, anticarcinogenic, and antiatherogenic properties. A validated RPLC method was developed for quantitative analysis of six major PMFs, namely nobiletin, tangeretin, sinensetin, 5,6,7,4'-tetramethoxyflavone, 3,5,6,7,3',4'-hexamethoxyflavone, and 3,5,6,7,8,3',4'-heptamethoxyflavone. The polar embedded LC stationary phase was able to fully resolve the six analogues. The developed method was fully validated in terms of linearity, accuracy, precision, sensitivity, and system suitability. The LOD of the method was calculated as 0.15 microg/mL and the recovery rate was between 97.0 and 105.1%. This analytical method was successfully applied to quantify the individual PMFs in four commercially available citrus peel extracts (CPEs). Each extract shows significant difference in the PMF composition and concentration. This method may provide a simple, rapid, and reliable tool to help reveal the correlation between the bioactivity of the PMF extracts and the individual PMF content.
authors: Discovery Chemistry, Hoffmann-La Roche Inc. Nutley, NJ, USA. zhenyu.wang@roche.com
authors: Zhenyu
authors: Z
authors: Wang
authors: Shiming
authors: S
authors: Li
authors: Stephen
authors: S
authors: Ferguson
authors: Robert
authors: R
authors: Goodnow
authors: Chi-Tang
authors: CT
authors: Ho
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D002957:Citrus / Q000033:anatomy & histology* / Q000737:chemistry*; D047309:Flavones / Q000032:analysis* / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008745:Methylation; D015394:Molecular Structure; D010100:Oxygen / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D047309:Flavones; D010936:Plant Extracts; D010100:Oxygen