PAM-microfabricated polyurethane scaffolds: in vivo and in vitro preliminary studies.
Polymeric scaffolds were realised with linear degradable PU in the form of square, hexagonal and octagonal grids. They were characterised in terms of their mechanical properties. Analysis shows that the mechanical properties of the scaffolds depend on their geometries which are easily modulated using PAM. In vitro biological assays showed that PU promotes the adhesion and proliferation of fibroblast cells and that cell activities are better on PU scaffolds than on PU films. In vivo implantation of PU and PLGA scaffolds and PU films demonstrated that the scaffolds are completely resorbed after three months with a slight inflammatory response, while the PU film was still present after six months with an intense granulomatous reaction.
Vozzi
Giovanni
G
Department of Chemical Engineering, Industrial Chemistry and Science of Materials, Interdepartmental Research Center E. Piaggio, Faculty of Engineering, University of Pisa, Via Diotisalvi 2, Pisa, Italy. g.vozzi@ing.unipi.it
Rechichi
Alfonsina
A
Dini
Francesca
F
Salvadori
Claudia
C
Vozzi
Federico
F
Burchielli
Silvia
S
Carlucci
Fabio
F
Arispici
Mario
M
Ciardelli
Gianluca
G
Giusti
Paolo
P
Ahluwalia
Arti
A
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D008422:Materials Testing; D011140:Polyurethanes / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013594:Syringes; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D011140:Polyurethanes