title: Anti-peptide antibody production elicited by in vitro immunization of human peripheral blood mononuclear cells.
abstract: Human monoclonal antibodies have great potential for use in the treatment of various diseases. We have established an in vitro immunization protocol for inducing antigen-specific antibody production from human peripheral blood mononuclear cells (PBMCs). In the in vitro immunization protocol, PBMCs are pretreated with L-leucyl-L-leucine methyl ester (LLME) to remove suppressive cells, and are sensitized and cultured with a soluble antigen in the presence of IL-2, IL-4 and muramyl dipeptide for 8 d, and then an antigen-specific antibody is produced. In this study, we examined the novel possibility of an in vitro immunization protocol, specifically, whether LLME-treated PBMCs can be sensitized with a peptide antigen to produce an anti-peptide antibody. The results indicate that antigen-specific immune responses were elicited by a peptide antigen derived from rice allergen, a cholera toxin B subunit, and TNF-alpha as a sensitizing antigen in in vitro immunization. These results suggest that the in vitro immunization protocol is applicable in the generation of an anti-peptide antibody against various antigens, including food allergens, foreign antigens, and self-antigens.
authors: Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors: Takashi
authors: T
authors: Tamura
authors: Kosuke
authors: K
authors: Tomimatsu
authors: Yoshinori
authors: Y
authors: Katakura
authors: Makiko
authors: M
authors: Yamashita
authors: Shin-ei
authors: SE
authors: Matsumoto
authors: Yoshihiro
authors: Y
authors: Aiba
authors: Yeon Suk
authors: YS
authors: Jung
authors: Yoshiichi
authors: Y
authors: Abe
authors: Tsukasa
authors: T
authors: Fujiki
authors: Kiichiro
authors: K
authors: Teruya
authors: Sanetaka
authors: S
authors: Shirahata
mesh_terms: D000485:Allergens / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000917:Antibody Formation; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000276:immunology; D004151:Dipeptides / Q000494:pharmacology; D006801:Humans; D007114:Immunization; D007376:Interleukin-2 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology*; D012275:Oryza / Q000276:immunology; D010455:Peptides / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D004151:Dipeptides; D007376:Interleukin-2; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha; C045139:leucyl-leucine-methyl ester; D002772:Cholera Toxin