[Influnce of Tinglizi on some neuroendocrine factors and type I and III collagen in ventricular remodeling induced by abdominal aortic banding in rats].
OBJECTIVE
To approach the influnce of Tinglizi on some neuroendocrine factors and type I and III collagen in ventricular remodeling induced by Abdominal Aortic Banding (AAB) in rats.


METHODS
Myocardial hypertrophy ventricular remodeling model was induced by abdominal aortic banding (AAB) in rats. After 30 day treatment, the systolic blood pressure (SBP) diastolic blood pressure (DBP) were measured; Then the ratios of LVW/BW (left ventricle weight/body weight), HW/BW (heart weight/body weight) were calculated; The Angiotensin II (Ang II) and Endothelin (ET-1) content in heart tissue and the Aldosterone (ALD) concentration of blood plasma were determined by radioimmunoassay. The content of type I and III collagen in myocardium were determined using immunohistochemical analysis. Hydroxy proline content in heart tissue was measured by hydrolysis method.


RESULTS
The experimental data demonstrated that Tinglizi could decrease SBP, DBP and the cardiac indexes of LVW/BW and HW/BW, significantly reduce the content of ANg II, ALD and ET, decrease the total collagen content and type I and III collagen (P<0.05).


CONCLUSIONS
Tiglizi can significantly attenuate the experimental ventricular remodeling; the mechanism is related with its ability to inhibit the activation of rennin-angiontensin-aldosterone system (RAAS) and systema nervosum sympatheticum (SNS), decrease the content of neuroendocrine factors (Ang II , ET, ALD).
Guo
Juan
J
Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. guojuan338@hotmail.com
Chen
Chang-xun
CX
D000450:Aldosterone / Q000097:blood; D000804:Angiotensin II / Q000096:biosynthesis*; D000818:Animals; D001012:Aorta, Abdominal / Q000503:physiopathology* / Q000601:surgery; D001794:Blood Pressure / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis; D003250:Constriction; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D016232:Endothelins / Q000096:biosynthesis; D017379:Hypertrophy, Left Ventricular / Q000378:metabolism / Q000503:physiopathology; D007150:Immunohistochemistry; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D020257:Ventricular Remodeling / Q000187:drug effects*
D004365:Drugs, Chinese Herbal; D016232:Endothelins; D000804:Angiotensin II; D000450:Aldosterone; D003094:Collagen