title: Separation of mesenchymal stem cells with magnetic nanosorbents carrying CD105 and CD73 antibodies in flow-through and batch systems.
abstract: The aim of this study is to develop magnetically loaded nanosorbents carrying specific monoclonal antibodies (namely CD105 and CD73) for separation of mesenchymal stem cells from cell suspensions. Super-paramagnetic magnetite (Fe3O4) nanoparticles were produced and then coated with a polymer layer containing carboxylic acid functional groups (average diameter: 153 nm and polydispersity index: 0.229). In order to obtain the nanosorbents, the monoclonal antibodies were immobilized via these functional groups with quite high coupling efficiencies up to 80%. These nanosorbents and also a commercially available one (i.e., microbeads carrying CD105 antibodies from Miltenyi Biotec., Germany) were used for separation of CD105+ and CD73+ mesenchymal stem cells from model cell suspension composed of peripheral blood (97.6%), human bone marrow cells (1.2%) and fibroblastic cells (1.2%). The initial concentrations of the CD105+ and CD73+ cells in this suspension were measured as 5.86% and 6.56%, respectively. A flow-through separation system and a very simple homemade batch separator unit were used. We were able to increase the concentration of CD105+ cells up to about 86% in the flow-through separation system with the nanosorbents produced in this study, which was even significantly better than the commercial one. The separation efficiencies were also very high, especially for the CD73+ cells (reached to about 64%) with the very simple and inexpensive homemade batch unit.
authors: Hacettepe University, Department of Chemical Engineering and Bioengineering Division and Biyomedtek, Ankara, Turkey.
authors: S
authors: S
authors: Odabaş
authors: F
authors: F
authors: Sayar
authors: G
authors: G
authors: Güven
authors: G
authors: G
authors: Yanikkaya-Demirel
authors: E
authors: E
authors: Pişkin
mesh_terms: D015720:5'-Nucleotidase / Q000276:immunology*; D000906:Antibodies / Q000737:chemistry / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D000071063:Endoglin; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D018448:Models, Immunological; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D011956:Receptors, Cell Surface / Q000276:immunology*; D015203:Reproducibility of Results
chemical_list: D000906:Antibodies; D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface; D015720:5'-Nucleotidase