title: Influence of dietary melatonin on photoreceptor survival in the rat retina: an ocular toxicity study.
abstract: Previous studies have shown that melatonin treatment increases the susceptibility of retinal photoreceptors to light-induced cell death. The purpose of this study was to evaluate under various conditions the potential toxicity of dietary melatonin on retinal photoreceptors. Male and female Fischer 344 (non-pigmented) and Long-Evans (pigmented) rats were treated with daily single doses of melatonin by gavage for a period of 14 days early in the light period or early in the dark period. In another group, rats were treated 3 times per week with melatonin early in the light period, and then exposed to high intensity illumination (1000-1500 lx; HII) for 2h, and then returned to the normal cyclic lighting regime. At the end of the treatment periods, morphometric measurements of outer nuclear layer thickness (ONL; the layer containing the photoreceptor cell nuclei) were made at specific loci throughout the retinas. In male and female non-pigmented Fischer rats, melatonin administration increased the degree of photoreceptor cell death when administered during the nighttime and during the day when followed by exposure to HII. There were some modest effects of melatonin on photoreceptor cell death when administered to Fischer rats during the day or night without exposure to HII. Melatonin treatment caused increases in the degree of photoreceptor cell death when administered in the night to male pigmented Long-Evans rats, but melatonin administration during the day, either with or without exposure to HII, had little if any effect on photoreceptor cell survival. In pigmented female Long-Evans rats, melatonin administration did not appear to have significant effects on photoreceptor cell death in any treatment group. The results of this study confirm and extend previous reports that melatonin increases the susceptibility of photoreceptors to light-induced cell death in non-pigmented rats. It further suggests that during the dark period, melatonin administration alone (i.e., no HII exposure) to pigmented male rats may have a toxic effect on retinal cells. These results suggest that dietary melatonin, in combination with a brief exposure to high intensity illumination, induces cellular disruption in a small number of photoreceptors. Chronic exposure to natural or artificial light and simultaneous intake of melatonin may potentially contribute to a significant loss of photoreceptor cells in the aging retina.
authors: Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA. allan-wiechmann@ouhsc.edu
authors: Allan F
authors: AF
authors: Wiechmann
authors: Colin F
authors: CF
authors: Chignell
authors: Joan E
authors: JE
authors: Roberts
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002940:Circadian Rhythm; D004305:Dose-Response Relationship, Drug; D005260:Female; D008029:Lighting; D008297:Male; D008550:Melatonin / Q000633:toxicity*; D010775:Photic Stimulation / Q000379:methods; D020419:Photoreceptor Cells, Vertebrate / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000139:chemically induced / Q000209:etiology / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D020318:Rats, Long-Evans; D012737:Sex Factors; D013045:Species Specificity
chemical_list: D008550:Melatonin