Development of an optical RNA-based aptasensor for C-reactive protein.
The development of a RNA-aptamer-based optical biosensor (aptasensor) for C-reactive protein (CRP) is reported. CRP is an important clinical biomarker; it was the first acute-phase protein to be discovered (1930) and is a sensitive systemic marker of inflammation and tissue damage. It has also a prognostic value for patients with acute coronary syndrome. The average concentration of CRP in serum is 0.8 ppm and it increases in response to a variety of inflammatory stimuli, such as trauma, tissue necrosis, infection and myocardial infarction. The interaction between the 44-base RNA aptamer and the target analyte CRP is studied. In particular, the influence of the aptamer immobilization procedure (chemistry, length, concentration), as well as the binding conditions, i.e., the influence on the binding of different buffers, the presence of Ca2+ ion and the specificity (against human serum albumin) have been evaluated. Using the best working conditions, we achieved a detection limit of 0.005 ppm, with good selectivity towards human serum albumin. Some preliminary experiments in serum are reported.
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Dipartimento di Chimica, Università degli Studi di Firenze, Polo Scientifico, Via della Lastruccia 3, 50019, Sesto Fiorentino, Florence, Italy.
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D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D002097:C-Reactive Protein / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry; D002138:Calibration; D009690:Nucleic Acid Conformation; D055095:Optics and Photonics
D052157:Aptamers, Nucleotide; D002097:C-Reactive Protein; D002118:Calcium