[Dendritic cells pulsed with glioma RNA induce immunity against intracranial gliomas].
To investigate the anti-tumor effect of dendritic cells (DC) pulsed with G422 glioblastomas RNA in mice bearing intracranially G422 glioblastomas.



DCs were pulsed in vitro with glioblastomas G422 cell RNA. The tumor-bearing mice were injected intratumorally or subcutaneously with pulsed DCs, PBS, non-pulsed DCs. The survival duration of mice was recorded. Serum levels of cytokine IFN-gamma, IL-2, IL-10, IL-4 were detected. Pathological examination was performed.



The survival duration of mice with DC-based vaccine increased significantly(P<0.01). The serum IFN-gamma level was increased (P<0.01) and IL-10 level was decreased (P<0.05) after treatment. Pathological examination showed necrotic tumor in the treatment mice.



DC vaccination can significantly increase survival duration of mice with intratumoral or subcutaneous administration of vaccines.
Yu
Jian-bo
JB
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Feng
Zhi-xian
ZX
Zhan
Ren-ya
RY
D000818:Animals; D001932:Brain Neoplasms / Q000276:immunology* / Q000628:therapy; D019496:Cancer Vaccines / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000637:transplantation*; D005909:Glioblastoma / Q000276:immunology* / Q000628:therapy; D007167:Immunotherapy / Q000379:methods*; D051379:Mice; D012334:RNA, Neoplasm / Q000276:immunology; D011897:Random Allocation; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D019496:Cancer Vaccines; D012334:RNA, Neoplasm