Immunomagnetic selection of progenitor cells from peripheral blood after thawing with an automatic system in a pediatric patient with a neuroblastoma.
BACKGROUND
Immunomagnetic selection of peripheral blood progenitor cells (PBPCs) in patients with tumoral infiltration in marrow makes it possible to reduce contamination of cellular concentrates, but this procedure cannot always be used, mainly because of the low cellular count in apheresis concentrates.


METHODS
In this case two cellular concentrates taken separately at two different times were selected and cryopreserved; they were thawed with an automatic instrument.


RESULTS
After manipulation, a selected concentrate containing 24.16 x 106 CD34+ cells with a purity of 90.15 percent was obtained; vitality after thawing and selection was 88 and 96 percent, respectively. The engraftment was achieved on Day +17 from the infusion of the previously selected PBPCs, as the literature also shows us.


CONCLUSIONS
The time passed between the infusion and the engraftment gives us evidence of the efficacy of immunomagnetic selection carried out after thawing 2 cell units that were collected at different times from the same patient. In this way, it has been possible to perform an autologous transplant in a patient in which CD34+ cells transplant is recommended, but from whom the number of collected cells after a single mobilization cycle would not have been sufficient for the engraftment.
Zinno
Francesco
F
Tor Vergata University, Rome, Italy. Francescozinno@virgilio.it
Landi
Fabiola
F
Aureli
Viviana
V
Donfrancesco
Alberto
A
Isacchi
Giancarlo
G
D018952:Antigens, CD34 / Q000097:blood; D002675:Child, Preschool; D004358:Drug Therapy / Q000379:methods; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation / Q000379:methods*; D009447:Neuroblastoma / Q000175:diagnosis / Q000628:therapy*
D018952:Antigens, CD34