title: Gene expression of AGS cells stimulated with released proteins by Helicobacter pylori.
abstract: OBJECTIVE
  Interactions between released proteins by Helicobacter pylori (H. pylori) and the cells of gastric epithelium to which it adheres may contribute to gastric inflammation and epithelial damage. The present study was performed to evaluate the gene expression of AGS gastric cancer cells stimulated with released proteins by H. pylori.
  
  
  METHODS
  Gene expression of AGS cells to the stimulation by H. pylori-released proteins (G27 strain) were monitored using oligonucleotide microarrays.
  
  
  RESULTS
  Eighty-eight genes (0.88%) and eight genes (0.08%) were up- or downregulated, respectively, by treating AGS cells with H. pylori-released proteins but not by H. pylori adhesion after 12 h of coculture. Out of the selected 40 up- and five downregulated genes, 29 upregulated genes classified as general RNA polymerase II transcription factor activity (GTF2B, PPARGC1A), SH3/SH2 adaptor activity (CRKL), transferase activity (ACLY, CRKL, PIGC, PLK4), and oxidoreductase activity (IDH1) were confirmed to be upregulated by released proteins and not by H. pylori adhesion by real-time reverse transcription-polymerase chain reaction. When the concentrated H. pylori-cultured supernatant prepared by our protocol was treated by boiling, the upregulations of 26 of these 29 genes (89.7%) except for CD160, ZNF268, and PSAT1 disappeared. This confirmed that most of these upregulations were caused by released proteins.
  
  
  CONCLUSIONS
  Host genes involving transcription, signaling and stress are significantly modulated by the proteins released by H. pylori. This might strengthen the gastroduodenal pathogenesis induced by H. pylori.
authors: Department of Internal Medicine, Seoul National University Bundang Hospital, Seoungnam, Gyeonggi-do, Korea.
authors: Nayoung
authors: N
authors: Kim
authors: Woong-Yang
authors: WY
authors: Park
authors: Jung Mogg
authors: JM
authors: Kim
authors: Ji Hyun
authors: JH
authors: Park
authors: Joo Sung
authors: JS
authors: Kim
authors: Hyun Chae
authors: HC
authors: Jung
authors: In Sung
authors: IS
authors: Song
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D004847:Epithelial Cells / Q000378:metabolism*; D015870:Gene Expression; D016480:Helicobacter pylori / Q000378:metabolism*; D013270:Stomach / Q000166:cytology; D014407:Tumor Cells, Cultured
chemical_list: D001426:Bacterial Proteins