Sequence analysis of mRNA transcripts encoding Jembrana disease virus Tat-1 in vivo.
Jembrana disease virus (JDV) is a lentivirus which replicates to very high titres in vivo and its Tat-1 protein has been shown to be a potent transactivator in vitro. Analysis of tat mRNA transcripts produced early in infection studies identified four predominant species which were generated by multiple splicing events. The use of a splice donor downstream of tat-1 was common indicating that a Tat-1 protein of 97 amino acids is expressed during the acute phase of Jembrana disease. The presence of an in-frame stop codon between tat-1 and tat-2 was identified in transcripts from three different strains of JDV confirming that expression of a single exon Tat-1 correlates with high viral titres in vivo. Sequence conservation in the regions of tat-1 that are critical for RNA binding and transcription activation in three different virus strains was high and the tat-2 sequences were completely conserved.
Setiyaningsih
Surachmi
S
School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Perth, Western Australia 6150, Australia.
Desport
Moira
M
Stewart
Meredith E
ME
Hartaningsih
Nining
N
Wilcox
Graham E
GE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D002522:Chlorocebus aethiops; D015696:Gene Products, tat / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D016180:Lentivirus Infections / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D022821:RNA Splice Sites; D012326:RNA Splicing; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA
D015696:Gene Products, tat; D022821:RNA Splice Sites; D012333:RNA, Messenger