title: An evaluation of the relative sensitivities of the venereal disease research laboratory test and the Treponema pallidum particle agglutination test among patients diagnosed with primary syphilis.
abstract: BACKGROUND
  Because definitive methods for diagnosing primary syphilis are limited, it is important to optimize the sensitivity of serodiagnosis.
  
  
  OBJECTIVE
  To determine the most sensitive testing approach to the diagnosis of primary syphilis, using the commonly available serologic tests: the Venereal Disease Research Laboratory (VDRL) test and the Treponema pallidum particle agglutination (TP-PA) test.
  
  
  METHODS
  Sensitivities of 2 serologic testing strategies for primary syphilis were compared among 106 darkfield-confirmed cases treated in San Francisco from January 2002 through December 2004.
  
  
  RESULTS
  The sensitivity of the diagnostic strategy using VDRL confirmed by TP-PA was 71% (95% CI, 61%-79%). Substituting Rapid Plasma Reagin test for VDRL in a subset of 51 patients produced the same sensitivity (71%; 95% CI, 56%-83%). The sensitivity of TP-PA as the first-line diagnostic test was 86% (95% CI, 78%-92%). The sensitivity of the former approach was significantly lower among HIV-positive patients, compared with HIV-negative patients (55% vs. 77%, P = 0.05).
  
  
  CONCLUSIONS
  The TP-PA test as the first-line diagnostic test yielded higher sensitivity for primary syphilis than did the use of the currently recommended strategy.
authors: California Department of Health Services, Sexually Transmitted Diseases (STD) Control Branch, 300 Frank H Ogawa Plaza, Suite 520, Oakland, CA 94612-2032, USA. Lcreegan@dhs.ca.gov
authors: Linda
authors: L
authors: Creegan
authors: Heidi M
authors: HM
authors: Bauer
authors: Michael C
authors: MC
authors: Samuel
authors: Jeffrey
authors: J
authors: Klausner
authors: Sally
authors: S
authors: Liska
authors: Gail
authors: G
authors: Bolan
mesh_terms: D000372:Agglutination Tests / Q000379:methods*; D001431:Bacteriological Techniques; D015658:HIV Infections / Q000150:complications; D006801:Humans; D012680:Sensitivity and Specificity; D013587:Syphilis / Q000175:diagnosis; D013588:Syphilis Serodiagnosis / Q000379:methods*