Comparison of GC and LC determinations of hexabromocyclododecane in biological samples - results from two interlaboratory comparison studies.
In order to assess the quality and comparability of results from determinations of 1,2,5,6,9,10-hexabromocyclododecane (HBCD) in biological samples, two interlaboratory comparison studies have been organized. Up to 13 laboratories determined either the total HBCD concentration, or concentrations of alpha-, beta- and gamma-HBCD, or both in cod liver oil, herring filet, salmon filet, butter and chicken meat. The laboratories were able to determine total HBCD concentrations in the marine samples with satisfying quality (RSD <35%). However, the analysis of samples with low HBCD contamination (<about 2ngg(-1) lipid weight) should be improved. No statistically significant differences were found between total HBCD concentrations obtained by LC-MS and GC-MS. To our knowledge, this is the first report on interlaboratory comparison studies concerning the determination of HBCD in biological samples comprising results from a sufficient number of participants to perform a statistical evaluation.
Haug
Line S
LS
Division of Environmental Medicine, Norwegian Institute of Public Health, P.O. Box 4404 Nydalen, N-0403 Oslo, Norway.
Thomsen
Cathrine
C
Liane
Veronica H
VH
Becher
Georg
G
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D003060:Cod Liver Oil / Q000032:analysis; D005399:Fishes / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006842:Hydrocarbons, Brominated / Q000032:analysis*; D007753:Laboratories / Q000592:standards; D013058:Mass Spectrometry
D006842:Hydrocarbons, Brominated; C089796:hexabromocyclododecane; D003060:Cod Liver Oil