C314T polymorphism in histamine N-methyltransferase gene and susceptibility to duodenal ulcer in Chinese population.
BACKGROUND
Histamine is a regulator of gastric acid secretion, which is involved in the development of duodenal ulcer (DU). Histamine is metabolized by both histamine N-methyltransferase (HNMT) and diamine oxidase, and its local action is terminated primarily by methylation which is catalyzed by HNMT.


METHODS
Polymerase chain reaction-restriction fragment length polymorphism assay was used to identify the polymorphism of the point mutation C314T of HNMT gene of 498 Chinese patients with DU and 151 healthy individuals.


RESULTS
In normal controls, the allele frequency of HNMT T314 was 3.3%, which was significantly lower than American Caucasians. The HNMT T314 allele was detected in 3.5% of the DU patients. In cases and controls, the frequency of C/C genotypes were 93.0% and 93.4%, respectively. The HNMT T/T genotype was not found in this population. No significant differences were seen in both genotype frequencies and allele frequencies between DU groups and controls. After stratified by H. pylori infection, they also could not reach significant differences in our current study.


CONCLUSIONS
The HNMT T314 allele frequency is lower in Chinese population than in American Caucasians. No association can be found in the involvement of HNMT C314T polymorphism in the susceptibility to duodenal ulcer.
Hailong
Cao
C
Department of Gastroenterology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
Cao
Haillong
H
Mei
Qiao
Q
Zhang
Lei
L
Xu
Jianming
J
D001483:Base Sequence; D016022:Case-Control Studies; D002681:China; D017931:DNA Primers; D004381:Duodenal Ulcer / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006637:Histamine N-Methyltransferase / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D017931:DNA Primers; D006637:Histamine N-Methyltransferase