Messenger RNA expression profiling using DNA microarray technology: diagnostic tool, scientific analysis or un-interpretable data?
Analysis of the transcriptome using DNA microarrays has become a standard approach for investigation of the molecular basis of human disease in both clinical and experimental settings. However, drawing conclusions from the wealth of data obtained has remained problematic. There have been difficulties with accurate reporting of results, with experimental reproducibility and with identifying and interpreting the biologically relevant information. In this review we discuss the successful use of DNA microarray technology in molecular medical research, and we highlight methods of addressing the issues of both reproducibility and biological interpretation.
Walker
Moira S
MS
YCR and Liz Dawn Pathology and Translational Sciences Centre, Leeds Institute of Molecular Medicine, St James's University Hospital, University of Leeds, Leeds LS9 7TF, UK.
Hughes
Thomas A
TA
D003627:Data Interpretation, Statistical; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D015203:Reproducibility of Results
D012333:RNA, Messenger