title: Bacterial cystitis is accompanied by increased peripheral thermal sensitivity in mice.
abstract: OBJECTIVE
  Visceral inflammation and pain associated with chemical cystitis produce increased sensitivity to noxious stimuli in the sacral dermatomes. We determined whether a similar sensitization occurs in response to bacterial cystitis.
  
  
  METHODS
  Bacterial cystitis was induced by intravesical instillation of Escherichia coli 1677 in female C57BL/6N and C3H/OuJ mice (Jackson Laboratories, Bar Harbor, Maine). C3H/HeJ mice (Jackson Laboratories) served as a control because C3H/HeJ mice lack functional toll-like receptor 4, which is an essential component of cellular recognition of bacterial lipopolysaccharide. Hind paw sensitivity to thermal stimulus was quantitatively determined 1, 2, 7 and 14 days after infection.
  
  
  RESULTS
  Intravesical instillation of E. coli produced infection in all strains of mice. Infection persisted in all C3H/OuJ and C3H/HeJ mice but it spontaneously cleared in some C57BL/6N mice. Increased sensitivity to thermal stimuli was observed in C57BL/6N and C3H/OuJ mice starting 1 to 2 days after E. coli instillation and it was still present 14 days after instillation. Increased sensitivity to thermal stimuli did not occur in C3H/HeJ mice.
  
  
  CONCLUSIONS
  E. coli induced cystitis produced increased sensitivity to peripheral thermal stimuli in mice with competent toll-like receptor 4.
authors: Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin, USA.
authors: Dale E
authors: DE
authors: Bjorling
authors: Zun-Yi
authors: ZY
authors: Wang
authors: Kyle
authors: K
authors: Boldon
authors: Wade
authors: W
authors: Bushman
mesh_terms: D000818:Animals; D003556:Cystitis / Q000150:complications* / Q000382:microbiology* / Q000503:physiopathology; D004927:Escherichia coli Infections / Q000150:complications* / Q000503:physiopathology; D005260:Female; D006358:Hot Temperature; D006930:Hyperalgesia / Q000209:etiology*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D017288:Pain Threshold / Q000502:physiology*; D010812:Physical Stimulation; D011930:Reaction Time