Extraction of flavonoids from Rhodiola sachlinesis A. Bor by UPE and the antioxidant activity of its extract.
Rhodiola sachlinesis, A. Bor is one of the most popular traditional Chinese medicines and has been proved to have many effective compounds, such as flavonoids, anthraglycosides, essential oil with cinnamic aldehyde and citral and organic acids. In this article, a new method of ultrahigh pressure extraction (UPE) was used to extract flavonoids from R. sachlinesis. Uniform Design was employed to get the optimum extraction conditions. The optimum UPE conditions were as follows: pressure was 500 MPa; ethanol concentration was 40%; solid/liquid ratio (g mL(-1)) was 1 : 70. The optimum extraction yield of flavonoids was 52.33 mg g(-1). The antioxidant activity of the crude extract was tested with DPPH assay. It showed a higher activity, compared with tertiary butyhydroquinone (TBHQ) at the same concentration (1 mg mL(-1)).
Zhang
Shouqin
S
Biological and Agricultural Engineering College, Jilin University, Changchun 130022, P.R. China.
Liu
Changjiao
C
Bi
Huimin
H
Wang
Changzheng
C
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D001713:Biphenyl Compounds / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification; D006834:Hydrazines / Q000737:chemistry; D010851:Picrates; D010946:Plants, Medicinal / Q000737:chemistry*; D029771:Rhodiola / Q000737:chemistry*
D000975:Antioxidants; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D016166:Free Radical Scavengers; D006834:Hydrazines; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl