Rapid culture and identification: a practical method for early preliminary laboratory diagnosis of sepsis.
This study describes the development of a method for rapid preliminary species identification of bacteria from positive blood culture vials. The method yielded preliminary identification results for 496 (92%) of 541 positive blood cultures within 5 h. The method was capable of identifying the most frequently isolated bacteria (i.e., Staphylococcus aureus, coagulase-negative staphylococci, Escherichia coli, Streptococcus pneumoniae and Enterococcus spp.) to the species level. The method can be established easily, with a materials cost of 2-5 Euros per sample.
Ozenci
V
V
Division of Clinical Microbiology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden. volkan.ozenci@karolinska.se
Tegmark-Wisell
K
K
Lundberg
C
C
Wretlind
B
B
D000465:Algorithms; D016470:Bacteremia / Q000175:diagnosis / Q000382:microbiology*; D001419:Bacteria / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D015373:Bacterial Typing Techniques / Q000379:methods* / Q000592:standards; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity