title: Dual-reporter phenotypic assay for human immunodeficiency viruses.
abstract: We have established a novel human immunodeficiency virus (HIV) tandem-reporter assay using HIV receptor-transduced NP-2 cells with long terminal repeat-controlled beta-galactosidase, inserted internal ribosome entry site, and secretary alkaline phosphatase genes. This assay allows users to detect replication of clinical isolates, indicating its useful application as an HIV phenotypic assay.
authors: Laboratory of Virus Immunology, Institute for Virus Research, Kyoto University, 53 Kawaramachi, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
authors: Keiko
authors: K
authors: Kajiwara
authors: Eiichi
authors: E
authors: Kodama
authors: Yasuko
authors: Y
authors: Sakagami
authors: Takeshi
authors: T
authors: Naito
authors: Masao
authors: M
authors: Matsuoka
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D019380:Anti-HIV Agents / Q000494:pharmacology; D002460:Cell Line; D017930:Genes, Reporter; D006678:HIV / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D015658:HIV Infections / Q000175:diagnosis*; D016325:HIV Long Terminal Repeat / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D014773:Virology / Q000379:methods*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D019380:Anti-HIV Agents; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase