title: Effect of hydroxyurea on procyclic Trypanosoma brucei: an unconventional mechanism for achieving synchronous growth.
abstract: Procyclic Trypanosoma brucei cells were synchronized with 0.2 mM hydroxyurea. The cells did not arrest at the G(1)/S boundary but proceeded through one round of replication and arrested near the end of S phase. The mitochondrial genome (kinetoplast DNA network) replicated, forming two progeny networks, but the repair of minicircle gaps was inhibited.
authors: Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
authors: Arnab Roy
authors: AR
authors: Chowdhury
authors: Zhixing
authors: Z
authors: Zhao
authors: Paul T
authors: PT
authors: Englund
mesh_terms: D000818:Animals; D004261:DNA Replication / Q000187:drug effects*; D018105:DNA, Kinetoplast / Q000235:genetics*; D016193:G1 Phase / Q000187:drug effects*; D006918:Hydroxyurea / Q000494:pharmacology*; D016196:S Phase / Q000187:drug effects*; D014346:Trypanosoma brucei brucei / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: D018105:DNA, Kinetoplast; D006918:Hydroxyurea