title: Optimization and validation of a chromatographic method for the simultaneous quantification of six bioactive compounds in Rhizoma et Radix Polygoni Cuspidati.
abstract: A reverse-phase HPLC method was developed for simultaneous quantification of six bioactive compounds in Rhizoma et Radix Polygoni Cuspidati. These compounds--polydatin (1), resveratrol (2), rhein (3), emodin (4), chrysophanol (5) and physcion (6)--were analysed from 24 authentic samples of the herb using UV HPLC. Based on the UV absorption characteristics of the six compounds, absorption wavelengths of 306 nm were chosen to quantify compounds 1 and 2, and 290 nm for compounds 3-6. A reliable and reproducible quantitative HPLC method for analysing authentic samples of Rhizoma et Radix Polygoni Cuspidati from different cultivation regions was developed. The results showed that the concentration of compound 1 in samples from Sichuan was almost 2 fold higher than that of samples acquired in Guangxi. Furthermore, compounds 3 and 5 were not found in all the samples tested. Thus, instead of using polydatin (1) and emodin (4) as markers for quality assessment, as in conventional practice, these findings show that compounds 2 and 6 are more suited to act as marker compounds for a more specific assessment of the quality of this herb.
authors: Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong Special Administrative Region, P. R. China.
authors: Guangsheng
authors: G
authors: Qian
authors: Sik-Yuen
authors: SY
authors: Leung
authors: GuangHua
authors: G
authors: Lu
authors: Kelvin Sze-Yin
authors: KS
authors: Leung
mesh_terms: D000880:Anthraquinones / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D004642:Emodin / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification; D045468:Fallopia japonica / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification; D015394:Molecular Structure; D015203:Reproducibility of Results; D000077185:Resveratrol; D027343:Rhizome / Q000737:chemistry*; D013267:Stilbenes / Q000032:analysis / Q000302:isolation & purification; D013678:Technology, Pharmaceutical / Q000379:methods / Q000592:standards
chemical_list: D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D013267:Stilbenes; C008905:physcione; D004642:Emodin; C027113:chrysophanic acid; D000077185:Resveratrol; C058229:polydatin; C020491:rhein