RNA cleavage by 2,9-diamino-1,10-phenanthroline PNA conjugates.
We report on the synthesis of 2,9-diamino-1,10-phenanthroline PNA conjugates as well as on their action in cleavage of a target RNA. Synthesis of the PNA conjugates are performed on solid support and the phenanthroline derivative is conjugated either to the amino-end or to a centrally positioned diaminopropionic acid in the PNA via a urea linker. Cleavage of the target RNA is achieved and compared to cleavage with the corresponding 2,9-dimethyl-1,10-phenanthroline and glycine conjugates.
Murtola
Merita
M
Department of Biosciences and Nutrition, Karolinska Institutet, NOVUM, S-14157 Huddinge, Sweden.
Ossipov
Dimitri
D
Sandbrink
Jessica
J
Strömberg
Roger
R
D020135:Peptide Nucleic Acids / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D012260:Ribonucleases / Q000138:chemical synthesis / Q000737:chemistry*
C527150:2,9-diamino-1,10-phenanthroline; D020135:Peptide Nucleic Acids; D010618:Phenanthrolines; D012313:RNA; D012260:Ribonucleases