Deinococcus misasensis and Deinococcus roseus, novel members of the genus Deinococcus, isolated from a radioactive site in Japan.
Two gamma- and UV-radiation resistant, Gram-positive, red- or pink-pigmented, rod-shaped, strictly aerobic, oxidase- and catalase-positive bacterial strains, TDMA-25T and TDMA-uv51T, were isolated from fresh water collected at Misasa, a radioactive site in Japan. Phylogenetic analysis based on 16S rRNA gene sequences placed both in a distinct lineage in the family Deinococcaceae, and the highest degrees of sequence similarity determined belonged to Deinococcus maricopensis LB-34T (88.8-89.3%), Deinococcus pimensis KR-235T (86.4-86.7%) and Deinococcus yavapaiensis KR-236T (86.1%). The DNA G+C content of the strains was 53-58 mol%. The major respiratory quinone was MK-8. The predominant fatty acids were C15:0 iso, C16:0 iso, C13:0 iso, C17:0 iso, C16:0, C13:0 anteiso, C15:0 and C12:0 iso. The strains degraded gelatin, casein, starch and Tween 80. Unique physiological characteristics, differences in their fatty acid profiles, and genotypic and phylogenetic features, differentiated strains TDMA-25T and TDMA-uv51T from closely related Deinococcus species. Hence, the two strains are described as novel species of the genus Deinococcus. The names Deinococcus misasensis sp. nov. (type strain TDMA-25T=JCM 14369=NBRC 102116=CCUG 53610) and Deinococcus roseus sp. nov. (type strain TDMA-uv51T=JCM 14370=NBRC 102117=CCUG 53611) are proposed.
Asker
Dalal
D
Department of Food Science, University of Massachusetts, 100 Holdsworth Way, Amherst, MA 01003, USA. dasker10@gmail.com
Awad
Tarek S
TS
Beppu
Teruhiko
T
Ueda
Kenji
K
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D034301:Deinococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000528:radiation effects; D005227:Fatty Acids / Q000032:analysis; D005720:Gamma Rays; D020459:Genes, rRNA; D007564:Japan; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014466:Ultraviolet Rays; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010860:Pigments, Biological; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; D002374:Catalase