title: In vitro and in vivo activities of Haplophyllum myrtifolium against Leishmania tropica.
abstract: This study aimed to evaluate the in vitro and in vivo leishmanicidal activity of an endemic Turkish plant and compare its efficacy with a reference drug. In addition to the in vitro activities of the ethanol, acidified and alkaloid extracts and furoquinoline alkaloids skimmianine and gamma-fagarine, in vivo antileishmanial activitiy of the acidified extract of Haplophyllum myrtifolium Boiss. (Rutaceae) were investigated against Leishmania tropica (L. tropica), a causative agent of cutaneous leishmaniasis. All the extracts and pure compounds showed in vitro inhibitory activity against the promastigotes of. L. tropica. The in vitro 50% inhibitory concentrations of y-fagarine, acidified extract, ethanol extract, skimmianine and alkaloid extract against promastigotes were determined as 8.7, 9.4, 10.9, 25.7 and 25.8 microg/ml respectively. In vivo results of Haplophyllum myrtifolium acidified extract showed that this plant has a limited effect on decreasing the lesion size of experimental mice infected with Leishmania tropica. To the best of our knowledge, this is the first time both the in vitro and in vivo antileishmanial activity of Haplophyllum mrytifolium have been reported in the same research.
authors: Vocational School of Health Services, Celal Bayar University, Manisa, Turkey. bluekaraca@yahoo.com
authors: Ipek
authors: I
authors: Ostan
authors: Hüsniye
authors: H
authors: Saglam
authors: M Emin
authors: ME
authors: Limoncu
authors: Hatice
authors: H
authors: Ertabaklar
authors: Seray Ozensoy
authors: SO
authors: Toz
authors: Yusuf
authors: Y
authors: Ozbel
authors: Ahmet
authors: A
authors: Ozbilgin
mesh_terms: D000279:Administration, Cutaneous; D000470:Alkaloids; D000818:Animals; D004353:Drug Evaluation, Preclinical; D000431:Ethanol; D006801:Humans; D007891:Leishmania / Q000187:drug effects* / Q000254:growth & development; D007896:Leishmaniasis / Q000188:drug therapy* / Q000469:parasitology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use*; D011804:Quinolines; D027821:Rutaceae; D014421:Turkey
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; D011804:Quinolines; D000431:Ethanol; C035932:skimmianine; C049193:gamma-fagarine