[Effect of lycopene on proliferation and cell cycle of hormone refractory prostate cancer PC-3 cell line].
OBJECTIVE
To investigate the effects of lycopene on proliferation of human prostate cancer cell line PC-3 and explore its effecting mechanism.


METHODS
The proliferation of PC-3 cells were analyzed by MTT and H3-TdR incorporation. The effects of cell cycle and apoptosis of synchronized cells were observed through flow cytometry. RT-PCR methods were used to explore the mRNA expression level of cyclin D1, bcl-2, bax.


RESULTS
The growth and DNA synthesis of PC-3 cell were inhibited with the lycopene concentration increased, and lycopene also could change the cell cycle distribution, i. e. increasing the proportion of G0/G1 phase and descending the proportion of S and G2/M phase, and induce the apoptosis. RT-PCR analysis showed that the mRNA expression level of cyclin D1 and bcl-2 were down regulated, while the level of bax was up regulated.


CONCLUSIONS
Lycopene can induce apoptosis of PC-3, change the cell cycle distribution and downregulate the expression of cyclin D1 and bcl-2 and upregulate the expression of bax and then restrain cell proliferation.
Wang
Aihong
A
West China School of Public Health, Sichuan University, Chengdu 610041, China. aihong-wang@sohu.com
Zhang
Lishi
L
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002338:Carotenoids / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D000077276:Lycopene; D008297:Male; D011471:Prostatic Neoplasms / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D019938:Cyclin D1; D002338:Carotenoids; D000077276:Lycopene