Production of transgenic goat (Capra hircus) with human Granulocyte Colony Stimulating Factor (hG-CSF) gene in Brazil.
In order to produce transgenic goats with hG-CSF, a total of 24 adult Saanen and 48 adult undefined breed goats were used as donors and recipients, respectively. Donors were estrus-synchronized with vaginal sponges and superovulated by a treatment with 200 mg FSH given twice daily in decreasing doses over 3 days starting 48 h before sponge removal. Ovulation was induced by injecting 100 microg GnRH 36 h after sponge removal. The recipients also received an estrus synchronization treatment. Donors were mated with fertile Saanen bucks and, approximately 72 h after sponge removal, zygotes were recovered surgically by flushing oviducts. The recovered zygotes were briefly centrifuged to a reliable visualization of the pronuclei. The DNA construct containing hG-CSF gene flanked by goat and bovine alphas1-casein sequences was injected into pronuclei of 129 zygotes. The microinjected embryos (3-6 per female) were transferred to 27 recipients. Ten recipients became pregnant and 12 kids were born. One transgenic male founder was identified in the group of kids. This is the first report of a birth of a transgenic goat in Latin America.
Freitas
Vicente J F
VJ
Laboratório de Fisiologia e Controle da Reprodução, Universidade Estadual do Ceará, Fortaleza, CE, Brazil. vjff@pq.cnpq.br
Serova
Irina A
IA
Andreeva
Lyudmila E
LE
Dvoryanchikov
Guennadi A
GA
Lopes
Edilson S
ES
Teixeira
Dárcio I A
DI
Dias
Luciene P B
LP
Avelar
Suely R G
SR
Moura
Raylene R
RR
Melo
Luciana M
LM
Pereira
Alexsandra F
AF
Cajazeiras
João B
JB
Andrade
Maria L L
ML
Almeida
Karlliely C
KC
Castro
Karlliely A
KA
Sousa
Francisco C
FC
Carvalho
Antonio C C
AC
Serov
Oleg L
OL
D000818:Animals; D030801:Animals, Genetically Modified / Q000196:embryology*; D001938:Brazil; D004624:Embryo Transfer; D005260:Female; D006041:Goats / Q000196:embryology / Q000235:genetics*; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics*; D008297:Male; D008845:Microinjections; D011247:Pregnancy; D015053:Zygote / Q000502:physiology
D016179:Granulocyte Colony-Stimulating Factor