title: Zinc potentiates neuronal nicotinic receptors by increasing burst duration.
abstract: Micromolar zinc potentiates neuronal nicotinic acetylcholine receptors (nAChRs) in a subtype-dependent manner. Zinc potentiates receptor function even at saturating agonist concentrations, without altering the receptor desensitization rate. Potentiation could occur through an increase in the number of available receptors, an increase in single-channel current amplitude, or an increase in single-channel open probability. To distinguish among these possibilities, we examined rat neuronal nAChRs expressed in Xenopus oocytes. Blockade of a large fraction of ACh activated alpha4beta4 or alpha4beta2 receptors by the open channel blocker hexamethonium failed to change the extent of potentiation by zinc, suggesting that zinc does not change the number of available receptors. The single-channel amplitudes of ACh (1 microM) activated alpha4beta4 receptors in outside-out patches were similar in the absence and the presence of 100 microM zinc (3.0 +/- 0.1 and 2.9 +/- 0.1 pA, respectively). To determine the effect of zinc on single-channel open probability, we examined alpha4beta4 receptors in cell-attached patches. The open probability at 100 nM ACh (0.011 +/- 0.002) was increased 4.5-fold by 100 microM zinc (0.050 +/- 0.008), accounting for most of the potentiation observed at the whole cell level. The increase in open probability was due to an increase in burst duration, which increased from 207 +/- 38 ms in the absence of zinc to 830 +/- 189 ms in the presence of zinc. Our results suggest that potentiation of neuronal nAChRs by zinc is due to a stabilization of the bursting states of the receptor.
authors: Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL 33101, USA.
authors: Bernard
authors: B
authors: Hsiao
authors: Karla B
authors: KB
authors: Mihalak
authors: Karl L
authors: KL
authors: Magleby
authors: Charles W
authors: CW
authors: Luetje
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004558:Electric Stimulation / Q000379:methods; D018738:Hexamethonium / Q000494:pharmacology; D015640:Ion Channel Gating / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D018733:Nicotinic Antagonists / Q000494:pharmacology; D009865:Oocytes; D018408:Patch-Clamp Techniques; D011336:Probability; D011978:Receptors, Nicotinic / Q000235:genetics / Q000502:physiology*; D013997:Time Factors; D014131:Trace Elements / Q000494:pharmacology*; D014162:Transfection; D014982:Xenopus laevis; D015032:Zinc / Q000494:pharmacology*
chemical_list: D018733:Nicotinic Antagonists; D011978:Receptors, Nicotinic; D014131:Trace Elements; C427403:alpha(4)beta(4) nicotinic receptor; D018738:Hexamethonium; D015032:Zinc; D000109:Acetylcholine