Quantitative telomeric overhang determination using a double-strand specific nuclease.
Telomeres terminate in 3' overhangs that function in end protection and the formation of t-loops. Determining the steps and factors involved in overhang processing is compromised by the inability to easily and accurately determine overhang size in the presence of many kilobases of double-stranded telomeric DNA. We here describe the use of a double-strand specific nuclease (DSN) that entirely digests double-stranded DNA including telomeres, leaving the overhangs intact so that they can be measured.
Zhao
Yong
Y
Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
Hoshiyama
Hirotoshi
H
Shay
Jerry W
JW
Wright
Woodring E
WE
D015139:Blotting, Southern; D004247:DNA / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D016615:Telomere / Q000737:chemistry* / Q000378:metabolism
D004247:DNA; D004706:Endodeoxyribonucleases