Analysis of the respiratory chain in Ethanologenic Zymomonas mobilis with a cyanide-resistant bd-type ubiquinol oxidase as the only terminal oxidase and its possible physiological roles.
The respiratory chain of the ethanologenic bacterium Zymomonas mobilis was investigated, in which the pyruvate-to-ethanol pathway has been demonstrated to be mainly responsible for NADH oxidation and the tricarboxylic acid cycle is incomplete. Membranes from cells cultivated under aerobic or anaerobic growth conditions showed dehydrogenase and oxidase activities for NADH, D-lactate and D-glucose and ubiquinol oxidase activity. Intriguingly, the NADH oxidase activity level of membrane fractions from cells grown aerobically was found to be higher than that of membrane fractions from Escherichia coli or Pseudomonas putida grown aerobically, indicating a crucial role of the respiratory chain in NADH oxidation in the organism. Cyanide-resistant terminal oxidase activity was observed and appeared to be due to a bd-type ubiquinol oxidase as the only terminal oxidase encoded by the entire genome. The terminal oxidase with a relatively strong ubiquinol oxidase activity exhibited remarkably weak signals of cytochrome d. Considering these findings and the presence of a type-II NADH dehydrogenase but not a type-I, a simple respiratory chain that generates less energymay have evolved in Z. mobilis.
Sootsuwan
Kaewta
K
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
Lertwattanasakul
Noppon
N
Thanonkeo
Pornthap
P
Matsushita
Kazunobu
K
Yamada
Mamoru
M
D000332:Aerobiosis; D000693:Anaerobiosis; D003486:Cyanides / Q000494:pharmacology*; D004579:Electron Transport / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D000431:Ethanol / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010088:Oxidoreductases / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism; D017355:Zymomonas / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism
D003486:Cyanides; D007773:Lactates; D011773:Pyruvates; D009243:NAD; D000431:Ethanol; D010088:Oxidoreductases; C432586:ubiquinol oxidase; D009245:NADH Dehydrogenase; D005947:Glucose