Labeling of oligonucleotides with DTPA and DOTA on solid phase.
Oligonucleotide conjugates labeled with metal chelates of diethylenetriaminepentaacetic acid (DTPA) and tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) were synthesized on solid phase using appropriate nucleosidic phosphoramidite building blocks (3, 4) and a modified deprotection-metal chelation protocol. The major differences on the properties of the oligonucleotide conjugates also are discussed.
Hovinen
Jari
J
PerkinElmer Life and Analytical Sciences, Turku Site, FIN-20101 Turku, Finland. jari.hovinen@perkinelmer.com
D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D004369:Pentetic Acid / Q000737:chemistry*
D006573:Heterocyclic Compounds, 1-Ring; D009841:Oligonucleotides; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; D004369:Pentetic Acid