[The protective effects of tenuigenin on the PC12 cells injury induced by H2O2].
OBJECTIVE
To study the protective effects of tenuigenin (TEN) on the PC12 cells injury induced by H2O2.


METHODS
Oxidative damage PC12 cells model was induced by H2O2. The survival rate of the cells was determined by MTT method. The content of LDH was determined by ultraviolet spectrophometry and the content of MDA and SOD activity was measured respectively by thiobarbituric acid and xanthine oxidese method.


RESULTS
tenuigenin could reduce obviously cells injury induced by H2O2. 10 mg L(-1) tenuigenin could improve the cells survival rate, reduce LDH releasing and MDA content, increase SOD activity.


CONCLUSIONS
tenuigenin have significantly potective effect on the PC12 cells injury induced by H2O2.
Sun
Gui-bo
GB
College of Life Science, South China Normal University, Guangzhou 510631, China. sunguibo@126.com
Deng
Xiang-chao
XC
Li
Chu-hua
CH
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D029608:Polygalaceae / Q000737:chemistry; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; C493220:tenuigenin; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase