[Effect of Ganoderma lucidum polysaccharides peptide on invasion of human lung carcinoma cells in vitro].
OBJECTIVE
To find out the effects of Ganoderma lucidum polysaccharides peptide (Gl-PP) on the invasion of the human lung carcinoma cell (PG cell).


METHODS
PG cells were pretreated with different concentration Gl-PP in vitro, using cell proliferation assay, cell migration assay, adhesion assay, zymography and RT-PCR, then the effects of Gl-PP on proliferation, motility, adhesion and MMP-9 activity and mRNA expression of PG cells were investigated in vitro.


RESULTS
Gl-PP did not directly inhibit PG cell proliferation in vitro. However, pretreated with Gl-PP, PG cells motility was inhibited significantly. PG cells adhesion was also inhibited. The activity of MMP-9 was inhibited in a dose-dependent manner, and the inhibited ratio was 41.53% at a dose of 100 mg/L Gl-PP. The mRNA expression of MMP-9 of pretreated PG cells was inhibited.


CONCLUSIONS
Gl-PP could suppress invasion of human lung carcinoma cells in vitro.
Cao
Qi Zhen
QZ
Department of Pharmacology, Peking University School of Basic Medical Sciences, Beijing 100083, China.
Lin
Shu Qian
SQ
Wang
Sai Zhen
SZ
D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D011509:Proteoglycans / Q000494:pharmacology*; D020940:Reishi
D011509:Proteoglycans; C093216:polysaccharide peptide; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9