A convenient solid-phase method for the synthesis of novel oligonucleotide-folate conjugates.
We describe the preparation of two batches of a polymer support for the incorporation of folic acid into oligonucleotides. The method permits the regioselective attachment of a target nucleic acid sequence through its 3'-end to either the alpha-or gamma-carboxyl group of L-glutamic acid, respectively. The supports have been tested in solid-phase synthesis of oligonucleotide-folate conjugates for cell delivery studies.
Kazanova
Eugenia V
EV
Department of Chemistry and A. N. Belozersky Institute of Physico-Chemical Biology, M. V. Lomonossov Moscow State University, Moscow, Russia.
Zubin
Eugeny M
EM
Kachalova
Anna V
AV
Volkov
Eugeny M
EM
Oretskaya
Tatiana S
TS
Stetsenko
Dmitry A
DA
Gottikh
Marina B
MB
D005492:Folic Acid / Q000032:analysis / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry
D009838:Oligodeoxyribonucleotides; D005492:Folic Acid