title: Griffonia simplicifolia isolectin B4 identifies a specific subpopulation of angiogenic blood vessels following contusive spinal cord injury in the adult mouse.
abstract: After traumatic spinal cord injury (SCI), disruption and plasticity of the microvasculature within injured spinal tissue contribute to the pathological cascades associated with the evolution of both primary and secondary injury. Conversely, preserved vascular function most likely results in tissue sparing and subsequent functional recovery. It has been difficult to identify subclasses of damaged or regenerating blood vessels at the cellular level. Here, adult mice received a single intravenous injection of the Griffonia simplicifolia isolectin B4 (IB4) at 1-28 days following a moderate thoracic (T9) contusion. Vascular binding of IB4 was maximally observed 7 days following injury, a time associated with multiple pathologic aspects of the intrinsic adaptive angiogenesis, with numbers of IB4 vascular profiles decreasing by 21 days postinjury. Quantitative assessment of IB4 binding shows that it occurs within the evolving lesion epicenter, with affected vessels expressing a temporally specific dysfunctional tight junctional phenotype as assessed by occludin, claudin-5, and ZO-1 immunoreactivities. Taken together, these results demonstrate that intravascular lectin delivery following SCI is a useful approach not only for observing the functional status of neovascular formation but also for definitively identifying specific subpopulations of reactive spinal microvascular elements.
authors: Kentucky Spinal Cord Injury Research Center, University of Louisville School of Medicine, Louisville, Kentucky 40292, USA.
authors: Richard L
authors: RL
authors: Benton
authors: Melissa A
authors: MA
authors: Maddie
authors: Danielle R
authors: DR
authors: Minnillo
authors: Theo
authors: T
authors: Hagg
authors: Scott R
authors: SR
authors: Whittemore
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D000367:Age Factors; D000818:Animals; D002196:Capillaries / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure; D002455:Cell Division / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL / Q000502:physiology*; D016253:Microscopy, Immunoelectron; D018919:Neovascularization, Physiologic / Q000502:physiology*; D037121:Plant Lectins; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology*; D013131:Spine / Q000098:blood supply* / Q000473:pathology; D019108:Tight Junctions / Q000502:physiology
chemical_list: C045475:Griffonia simplicifolia lectins; D037121:Plant Lectins