title: Growth hormone, insulin-like growth factor I, and leptin interaction in human cultured lutein granulosa cells steroidogenesis.
abstract: OBJECTIVE
  To investigate the effect of leptin on the actions of growth hormone (GH) and insulin-like growth factor-I (IGF-I) in ovarian steroidogenesis in vitro.
  
  
  METHODS
  In vitro cell culture study.
  
  
  METHODS
  Research laboratory of a university hospital.
  
  
  METHODS
  Luteinized granulosa cells (GC) were obtained from 20 healthy women undergoing ovarian stimulation for IVF treatment.
  
  
  METHODS
  Granulosa cells were cultured in serum free conditions for 72 hours with either GH or IGF-I, or their combination, in the absence or presence of leptin (1, 10, and 100 ng/mL).
  
  
  METHODS
  At 24 and 72 hours of the culture estradiol (E(2)) and progesterone (P) concentrations were measured in culture supernatants by enzyme immunoassays.
  
  
  RESULTS
  Leptin at the doses of 1 and 10 ng/mL significantly stimulated, whereas at the dose of 100 ng/mL it significantly suppressed E(2) and P production as compared to control. Estradiol production was significantly stimulated by both GH (72 hours) and IGF-I (24 and 72 hours) and that of P only by IGF-I (72 hours). Leptin at the low doses enhanced, whereas at the high dose it suppressed the effect of GH or IGF-I on E(2) and P synthesis.
  
  
  CONCLUSIONS
  These results demonstrate for the first time that leptin can modulate the effect of GH on steroids production by human luteinized GC in culture.
authors: Department of Obstetrics and Gynecology, Medical School, University of Thessalia, Larissa, Greece.
authors: Maria
authors: M
authors: Karamouti
authors: Panagoula
authors: P
authors: Kollia
authors: Athanasios
authors: A
authors: Kallitsaris
authors: Nikolaos
authors: N
authors: Vamvakopoulos
authors: Georgios
authors: G
authors: Kollios
authors: Ioannis E
authors: IE
authors: Messinis
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D019382:Human Growth Hormone / Q000378:metabolism*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D020738:Leptin / Q000378:metabolism*; D014975:Lutein / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis; D013256:Steroids / Q000096:biosynthesis*
chemical_list: D020738:Leptin; D013256:Steroids; D019382:Human Growth Hormone; D011374:Progesterone; D004958:Estradiol; D007334:Insulin-Like Growth Factor I; D014975:Lutein