title: IdeE reduces the bactericidal activity of equine neutrophils for Streptococcus equi.
abstract: Streptococcus equi (S. equi) causes equine strangles, a highly contagious and widespread purulent lymphadenitis of the head and neck. Highly resistant to phagocytosis, it produces long extracellular chains in affected lymph nodes. In a screen of clones reactive with convalescent serum from a gene library of S. equi CF32 we identified IdeE, an IgG-endopeptidase and homologue of the leucocyte receptor Mac-1 (CD11b). IdeE is expressed during S. equi infection eliciting both serum and mucosal antibody responses which persisted at significant levels in serum for over 200 days. Release from S. equi into culture medium was detected during the exponential phase of growth. The closely related Streptococcus zooepidemicus appeared to store the protein but not to release it. Antiphagocytic activity for equine neutrophils was dose-dependent and neutralized by IdeE-specific antiserum. Biotinylated IdeE bound weakly to about 77% of purified equine neutrophils and strongly to the remainder.
authors: Department of Veterinary Science, University of Kentucky, Lexington, KY 40546-0099, USA. jtimoney@email.uky.edu
authors: John F
authors: JF
authors: Timoney
authors: Jiande
authors: J
authors: Yang
authors: Jie
authors: J
authors: Liu
authors: Catherine
authors: C
authors: Merant
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000502:physiology*; D001770:Blood Bactericidal Activity; D003546:Cysteine Endopeptidases / Q000502:physiology*; D006736:Horses; D007074:Immunoglobulin G / Q000378:metabolism*; D016177:Macrophage-1 Antigen / Q000502:physiology*; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis; D018502:Streptococcus equi / Q000276:immunology*
chemical_list: D001426:Bacterial Proteins; D007074:Immunoglobulin G; C442815:Mac-1-like protein, Streptococcus; D016177:Macrophage-1 Antigen; D003546:Cysteine Endopeptidases