Multi-well chip for forming a uniform embryoid body in a tiny droplet with mouse embryonic stem cells.
A multi-well chip (MWC) is described by which mouse embryonic carcinoma (EC) stem cells form a comparatively more rapid and uniform embryoid body (EB) over the conventional hanging drop (HD) method. The newly developed MWC consists of an array of extruded through-holes, each of which holds a droplet of the cell suspension. The study found that the small curvature radius of the droplet in the MWC improved the EB formation rate of a hanging drop from 70% to 98%. Furthermore, the EBs formed by the MWC were uniformly round in shape regardless of the number of suspended cells ranging from 0.5 x 10(3) to 20 x 10(3). The ratio of beating colonies from the MWC was over 2-fold larger than that from HD. The experiments demonstrate that the MWC will be a valuable experimental tool for robust and reproducible EB-based differentiation of a defined number of ES cells.
Kim
Choong
C
Nano-Bio Research Center, Korea Institute of Science Technology, Songbuk-Gu, Seoul, Korea.
Lee
In Hye
IH
Lee
Kangsun
K
Ryu
Sung Shin
SS
Lee
Sang Ho
SH
Lee
Kyu-Jung
KJ
Lee
Jinwoo
J
Kang
Ji Yoon
JY
Kim
Tae Song
TS
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002449:Cell Aggregation; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice
D015415:Biomarkers