title: Determination of aflatoxins in hazelnuts by various sample preparation methods and liquid chromatography-tandem mass spectrometry.
abstract: A sensitive and reliable liquid chromatography-tandem mass spectrometric with electrospray ionization method for determining aflatoxins in hazelnuts has been developed. Three different extraction techniques, such as homogenization, ultrasonic extraction, and matrix solid phase dispersion have been tested and compared in terms of recovery, matrix effect, accuracy and precision. Ultrasound extraction was the most performing sample preparation method. Absolute recoveries for analytes and I.S. ranged from 93 to 101%. Accuracy and precision were calculated using matrix matched calibration, and ranged 91-102% and 2-11%, respectively. CC alpha and CC beta for aflatoxin B1 (EU limit=2 microg/kg) were 2.15 and 2.33 microg/kg, respectively. A ruggedness test performed on three other matrices demonstrated that sonication time was critical and a matrix matched calibration must be constructed for every sort of matrix.
authors: Department of Chemistry, Sapienza University, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors: Alessandro
authors: A
authors: Bacaloni
authors: Chiara
authors: C
authors: Cavaliere
authors: Francesca
authors: F
authors: Cucci
authors: Patrizia
authors: P
authors: Foglia
authors: Roberto
authors: R
authors: Samperi
authors: Aldo
authors: A
authors: Laganà
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D005591:Chemical Fractionation / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D031211:Corylus / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014465:Ultrasonics
chemical_list: D000348:Aflatoxins