title: Smart hydrogel particles: biomarker harvesting: one-step affinity purification, size exclusion, and protection against degradation.
abstract: Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and (c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis.
authors: CRO-IRCCS National Cancer Institute, Aviano, Italy.
authors: Alessandra
authors: A
authors: Luchini
authors: David H
authors: DH
authors: Geho
authors: Barney
authors: B
authors: Bishop
authors: Duy
authors: D
authors: Tran
authors: Cassandra
authors: C
authors: Xia
authors: Robert L
authors: RL
authors: Dufour
authors: Clinton D
authors: CD
authors: Jones
authors: Virginia
authors: V
authors: Espina
authors: Alexis
authors: A
authors: Patanarut
authors: Weidong
authors: W
authors: Zhou
authors: Mark M
authors: MM
authors: Ross
authors: Alessandra
authors: A
authors: Tessitore
authors: Emanuel F
authors: EF
authors: Petricoin
authors: Lance A
authors: LA
authors: Liotta
mesh_terms: D015415:Biomarkers; D002846:Chromatography, Affinity / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods*; D020100:Hydrogels; D006868:Hydrolysis
chemical_list: D015415:Biomarkers; D020100:Hydrogels