title: Catalase T and Cu,Zn-superoxide dismutase in the acetic acid-induced programmed cell death in Saccharomyces cerevisiae.
abstract: To investigate the role of catalase and superoxide dismutase (SOD) in the acetic acid (AA) induced yeast programmed cell death (AA-PCD), we compared Saccharomyces cerevisiae cells (C-Y) and cells individually over-expressing catalase T (CTT1-Y) and Cu,Zn-SOD (SOD1-Y) with respect to cell survival, hydrogen peroxide (H2O2) levels and enzyme activity as measured up to 200 min after AA treatment. AA-PCD does not occur in CTT1-Y, where H2O2 levels were lower than in C-Y and the over-expressed catalase activity decreased with time. In SOD1-Y, AA-PCD was exacerbated; high H2O2 levels were found, SOD activity increased early, remaining constant en route to AA-PCD, but catalase activity was strongly reduced.
authors: Dipartimento di Scienze per la Salute, Università del Molise, Via De Sanctis I, Campobasso, Italy.
authors: Nicoletta
authors: N
authors: Guaragnella
authors: Lucia
authors: L
authors: Antonacci
authors: Sergio
authors: S
authors: Giannattasio
authors: Ersilia
authors: E
authors: Marra
authors: Salvatore
authors: S
authors: Passarella
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002374:Catalase / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D002374:Catalase; D013482:Superoxide Dismutase; D019342:Acetic Acid