title: Multiplex PCR that can distinguish between immunologically cross- reactive serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains.
abstract: We describe a highly sensitive and specific multiplex PCR, based on capsular loci and the species specific apxIV gene, that unequivocally differentiates serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains that are cross-reactive in conventional immunological tests.
authors: Department of Paediatrics, Imperial College London, St. Mary's Campus, London W2 1PG, United Kingdom.
authors: L
authors: L
authors: Zhou
authors: S C P
authors: SC
authors: Jones
authors: Ø
authors: Ø
authors: Angen
authors: J T
authors: JT
authors: Bossé
authors: J H E
authors: JH
authors: Nash
authors: J
authors: J
authors: Frey
authors: R
authors: R
authors: Zhou
authors: H C
authors: HC
authors: Chen
authors: J S
authors: JS
authors: Kroll
authors: A N
authors: AN
authors: Rycroft
authors: P R
authors: PR
authors: Langford
mesh_terms: D000189:Actinobacillus Infections / Q000382:microbiology*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D016667:Bacterial Capsules / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D003429:Cross Reactions / Q000276:immunology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: C122177:ApxIVA protein, Actinobacillus pleuropneumoniae; D001426:Bacterial Proteins; D004269:DNA, Bacterial