Manganese peroxidase production by immobilized Phanerochaete chrysosporium BKM-F-1767 in a cell bioreactor.
Manganese-dependent peroxidase (MnP) production was performed in an immobilized cell bioreactor in which Phanerochaete chrysosporium BKM-F-1767 was immobilized on polystyrene foam. The immobilized cell culture yielded significantly greater MnP activity than the conventional stationary liquid culture. Cultivation was carried out in batch mode; the effect of glucose concentration was investigated and growth kinetics parameters were found as, micromax=0.59 day(-1), Ks=0.33 g/L and Kss=14.5. Batch operation led to maximum MnP (770.82 U/L) in the culture medium containing 0.05% Tween 80, 10 g/L glucose, and 174 microM Mn2+ at 37 degrees C and pH 4.5. Enzyme productivity was obtained as 110.12 U/day/L.
Urek
Raziye Ozturk
RO
Faculty of Education, Department of Chemistry, Dokuz Eylul University, Buca, Izmir, Turkey.
Pazarlioglu
Nurdan Kasikara
NK
D019149:Bioreactors; D002478:Cells, Cultured; D018914:Cells, Immobilized; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010544:Peroxidases / Q000096:biosynthesis*; D020075:Phanerochaete / Q000166:cytology / Q000201:enzymology*; D011137:Polystyrenes / Q000737:chemistry; D013696:Temperature
D011137:Polystyrenes; D010544:Peroxidases; C051129:manganese peroxidase; D005947:Glucose