title: Endometriosis and human infertility: a new investigation into the role of eutopic endometrium.
abstract: BACKGROUND
  Endometriosis is related to infertility even in the absence of mechanical alterations of the reproductive tract. Even though the pathogenesis of this phenomenon is still unclear, an impaired endometrial receptivity has been recently suggested. The aim of the present study was to investigate if endometriotic peritoneal fluids (EPF) could interfere with endometrial stromal cell (ESC) decidualization and if tumor necrosis factor (TNF)-alpha could be involved in the EPF effect.
  
  
  METHODS
  Eutopic ESC were isolated from patients with or without endometriosis. ESC were treated with 17beta-estradiol 10(-8) M and 6alpha-methyl-17alpha-hydroxyprogesteroneacetate 2x10(-7) M for 16 days. In vitro decidualization was morphologically and biochemically assessed. We analysed whether ESC decidualization could be affected by EPF or peritoneal fluids from control patients (CPF), with or without soluble TNF-alpha receptor 1 (sTNFR-1).
  
  
  RESULTS
  Compared with ESC from control patients, eutopic ESC from patients with endometriosis showed an impaired decidualization. Decidualization of normal ESC was morphologically normal but biochemically abnormal in the presence of EPF, which was able to decrease the secretion of decidualization markers. sTNFR-1 was able to partially counteract this effect.
  
  
  CONCLUSIONS
  In endometriosis, the milieu surrounding the uterine cavity may be involved in impaired eutopic ESC decidualization, partially due to increased peritoneal levels of TNF-alpha.
authors: Cattedra di Fisiopatologia della Riproduzione Umana, Università Cattolica del Sacro Cuore (UCSC), 00168 Roma, Italy. fm1810@inwind.it
authors: Francesca
authors: F
authors: Minici
authors: Federica
authors: F
authors: Tiberi
authors: Anna
authors: A
authors: Tropea
authors: Mariateresa
authors: M
authors: Orlando
authors: Maria Francesca
authors: MF
authors: Gangale
authors: Federica
authors: F
authors: Romani
authors: Sebastiano
authors: S
authors: Campo
authors: Adriano
authors: A
authors: Bompiani
authors: Antonio
authors: A
authors: Lanzone
authors: Rosanna
authors: R
authors: Apa
mesh_terms: D000328:Adult; D001202:Ascitic Fluid / Q000502:physiology; D003656:Decidua / Q000187:drug effects / Q000502:physiology; D004715:Endometriosis / Q000150:complications / Q000503:physiopathology*; D004717:Endometrium / Q000166:cytology / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000503:physiopathology*; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000378:metabolism; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology; D011388:Prolactin / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
chemical_list: C032231:IGFBP1 protein, human; D018970:Insulin-Like Growth Factor Binding Protein 1; D014409:Tumor Necrosis Factor-alpha; D004958:Estradiol; D011388:Prolactin; D017258:Medroxyprogesterone Acetate