Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061.
The four overlapping cosmids from the rubradirin producer, Streptomyces achromogenes var rubradiris NRRL 3061, have 58 ORFs within a 105.6 kb fragment. These ORFs harbored essential genes responsible for the formation and attachment of four distinct moieties, along with the genes associated with regulatory, resistance, and transport functions. The PKS (rubA) and glycosyltransferase (rubG2) genes were disrupted in order to demonstrate a complete elimination of rubradirin production. The rubradirin biosynthetic pathway was proposed based on the putative functions of the gene products, the functional identification of sugar genes, and the mutant strains.
Kim
Chun-Gyu
CG
Department of Pharmaceutical Engineering, Inje University, Kimhae, Kyung-nam, Republic of Korea.
Lamichhane
Janardan
J
Song
Kwang-Il
KI
Nguyen
Van D
VD
Kim
Dong-Hee
DH
Jeong
Tae-Sung
TS
Kang
Sung-Ho
SH
Kim
Kyoung-Wook
KW
Maharjan
Jyoti
J
Hong
Young-Soo
YS
Kang
Jae Seon
JS
Yoo
Jin-Cheol
JC
Lee
Jung-Joon
JJ
Oh
Tae-Jin
TJ
Liou
Kwangkyoung
K
Sohng
Jae Kyung
JK
D062365:Aminobenzoates / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001483:Base Sequence; D003360:Cosmids; D004269:DNA, Bacterial / Q000032:analysis; D005285:Fermentation; D020868:Gene Silencing; D006027:Glycosides / Q000096:biosynthesis; D062385:Hydroxybenzoates; D008969:Molecular Sequence Data; D005810:Multigene Family; D009285:Naphthoquinones / Q000378:metabolism; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
D062365:Aminobenzoates; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D006027:Glycosides; D062385:Hydroxybenzoates; D009285:Naphthoquinones; C024513:rubranitrose; C031509:3-amino-5-hydroxybenzoic acid; C002857:rubradirin