title: [The building of chip-based capillary electrophoresis platform and application].
abstract: OBJECTIVE
  To detect the point mutations by chip-based capillary electrophoresis and to provide a rapid and sensitive technique detection for beta-thalassemia.
  
  
  METHODS
  Multiplex primer-extension reaction was used to amplify the common loci of the samples for beta-thalassemia. The reaction products were detected by the chip-based capillary electrophoresis and the genotypes of the samples were discrened.
  
  
  RESULTS
  A system was constructed to detect the point mutations of beta-thalassemia by chip-based capillary electrophoresis, and the technology was ralidated by the patients' samples and the results coincided with those of detection kit.
  
  
  CONCLUSIONS
  Beta-thalassemia can be detected by chip-based capillary electrophoresis rapidly with a small amount of samples. It would be a new detection method of the genetic disorders.
authors: Department of Obstetrics & Gynecology, Xinan Hospital, the Third Military Medical University, Chongqing, 400038 PR China.
authors: Hua
authors: H
authors: Hu
authors: Qiang
authors: Q
authors: Xiong
authors: Cai-xia
authors: CX
authors: Li
authors: Hua-fang
authors: HF
authors: Gao
authors: Qian
authors: Q
authors: Yang
authors: Zhi-qing
authors: ZQ
authors: Liang
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D030342:Genetic Diseases, Inborn / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D017086:beta-Thalassemia / Q000175:diagnosis* / Q000235:genetics