Proteomic identification of tyrosine nitration targets in kidney of spontaneously hypertensive rats.
Nitrosative and oxidative stress are implicated in the development of hypertension. Events in the renal medulla may play a key role in the development and progression of hypertension. This may arise through disruption of nitric oxide signalling in the medulla and be accompanied by enhanced nitrosative and oxidative stress as indicated by the presence of proteins containing 3-nitrotyrosine. Here we demonstrate enhanced protein nitration in the medulla of spontaneously hypertensive rats. We have identified several nitrated proteins with both varied subcellular location and functional roles. These proteins are involved in nitric oxide signalling, antioxidant defense and energy metabolism. Moreover, increased nitration was observed in conjunction with enhanced oxidative damage as evidenced by the presence of protein carbonyl oxidative stress biomarkers. Our results suggest that kidney medulla is subject to enhanced nitrosative and oxidative stress, and that resulting protein modifications may contribute to the progression of hypertension.
Tyther
Raymond
R
Proteomics Research Group, Department of Biochemistry, University College Cork, Ireland.
Ahmeda
Ahmad
A
Johns
Edward
E
Sheehan
David
D
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D003600:Cytosol / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D007668:Kidney / Q000378:metabolism*; D007679:Kidney Medulla / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism*; D050050:Protein Carbonylation; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D014443:Tyrosine / Q000031:analogs & derivatives / Q000276:immunology / Q000378:metabolism*
D011506:Proteins; C002744:3-nitrotyrosine; D014443:Tyrosine; D009584:Nitrogen