Real-time monitoring of nitric oxide (NO) and pO2 levels under ischemic conditions associated with small bowel ischemia/reperfusion injury using selective electrodes for NO and oxygen molecules.
The present study demonstrated the feasibility of monitoring nitric oxide (NO) and pO2 levels under ischemic conditions associated with small bowel ischemia/reperfusion (I/R) injury through the use of selective electrodes for NO and oxygen molecules. NO levels gradually increased during ischemia. When reperfusion was started, the NO level decreased suddenly and returned to pre-ischemia values within 10 minutes. After clamping, pO2 decreased rapidly. When reperfusion was started, pO2 increased suddenly, returning to pre-ischemia values within 10 minutes. We concluded that it is feasible to monitor NO and pO2 levels under ischemic conditions of small bowel I/R injury through the use of electrodes selective for NO and oxygen molecules.
Watanabe
T
T
Department of Digestive and General Surgery, St. Marianna University, School of Medicine, Kawasaki, Kanagawa, Japan. taiji_watanabe@marianna-u.ac.jp
Owada
S
S
Kobayashi
H
H
Ishiuchi
A
A
Nakano
H
H
Asakuta
T
T
Shimamura
T
T
Asano
T
T
Koizumi
S
S
Jinnouchi
Y
Y
Katayama
M
M
Kamibayasi
M
M
Murakami
E
E
Otsubo
T
T
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D004195:Disease Models, Animal; D004566:Electrodes; D007421:Intestine, Small / Q000098:blood supply*; D008297:Male; D008991:Monitoring, Physiologic / Q000379:methods; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism*
D015415:Biomarkers; D009569:Nitric Oxide; D010100:Oxygen