title: MsrA protects cardiac myocytes against hypoxia/reoxygenation induced cell death.
abstract: Reactive oxygen species (ROS) are critical in tissue responses to ischemia-reperfusion. The enzyme methionine sulfoxide reductase-A (MsrA) is capable of protecting cells against oxidative damage by reversing damage to proteins caused by methionine oxidation or by decreasing ROS through a scavenger mechanism. The current study employed adenovirus mediated over-expression of MsrA in primary neonatal rat cardiac myocytes to determine the effect of this enzyme in protecting against hypoxia/reoxygenation in this tissue. Cells were transduced with MsrA encoding adenovirus and subjected to hypoxia/reoxygenation. Apoptotic cell death was decreased by greater than 45% in cells over-expressing MsrA relative to cells transduced with a control virus. Likewise total cell death as determined by levels of LDH release was dramatically decreased by MsrA over-expression. These observations indicate that MsrA is protective against hypoxia/reoxygenation stress in cardiac myocytes and point to MsrA as an important therapeutic target for ischemic heart disease.
authors: Florida Atlantic University, College of Biomedical Science, 777 Glades Road, Boca Raton, FL 33431, USA.
authors: H M
authors: HM
authors: Prentice
authors: I A
authors: IA
authors: Moench
authors: Z T
authors: ZT
authors: Rickaway
authors: C J
authors: CJ
authors: Dougherty
authors: K A
authors: KA
authors: Webster
authors: H
authors: H
authors: Weissbach
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D002316:Cardiotonic Agents / Q000378:metabolism*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology*; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D002316:Cardiotonic Agents; D017382:Reactive Oxygen Species; D010088:Oxidoreductases; C488525:MsrA protein, rat