title: Assessment of intra-arterial injected autologous bone marrow mononuclear cell distribution by radioactive labeling in acute ischemic stroke.
abstract: OBJECTIVE
  To evaluate the feasibility of monitoring the autologous mononuclear bone marrow (ABMMN) cells implanted into the brain after acute ischemic stroke by the technique of labeling with Tc-99m-HMPAO.
  
  
  METHODS
  A 37-year-old man presented with aphasia, right-side hypoesthesia, and right homonymous hemianopsia after an acute ischemic stroke of the left middle cerebral artery. He was included in an autologous bone marrow mononuclear cell-based therapy research protocol about the safety of intra-arterial autologous bone marrow mononuclear cell transplantation for acute ischemic stroke. Nine days after the stroke he received 3.0 x 10(7) ABMMN cells delivered into the left cerebral middle artery via a balloon catheter. Approximately 1% of these cells were labeled with 150 MBq (4 mCi) Tc-99m by incubation with hexamethylpropylene amine oxime (HMPAO).
  
  
  RESULTS
  Brain perfusion images with Tc-99m ECD demonstrated hypoperfusion in the left temporal and parietal regions. The perfusion brain images were compared with tomographic views of the brain obtained 8 hours after ABMMN-labeled cell delivery, revealing intense accumulation of the ABMMN-labeled cells in the ipsilateral hemisphere. A whole-body scan was done and showed left brain, liver, and spleen uptake.
  
  
  CONCLUSIONS
  Our results showed that Tc-99m HMPAO can be used to label ABMMN cells for in vivo cell visualization, and that brain SPECT imaging with labeled ABMMN cells is a feasible noninvasive method for studying the fate of transplanted cells in vivo. Additionally, our findings demonstrate the localization of these intra-arterially injected cells.
authors: Hospital Procardiaco, Rio de Janeiro, Brazil. medicina.nuclear@procardiaco.com.br
authors: Patricia Lavatori
authors: PL
authors: Correa
authors: Claudio T
authors: CT
authors: Mesquita
authors: Renata M
authors: RM
authors: Felix
authors: Jader C
authors: JC
authors: Azevedo
authors: Gustavo B
authors: GB
authors: Barbirato
authors: Carlos Henrique
authors: CH
authors: Falcão
authors: Constantino
authors: C
authors: Gonzalez
authors: Maria Lucia
authors: ML
authors: Mendonça
authors: Aquiles
authors: A
authors: Manfrim
authors: Gabriel
authors: G
authors: de Freitas
authors: Cristina C
authors: CC
authors: Oliveira
authors: Daniel
authors: D
authors: Silva
authors: Dione
authors: D
authors: Avila
authors: Radovan
authors: R
authors: Borojevic
authors: Suzana
authors: S
authors: Alves
authors: Amarino C
authors: AC
authors: Oliveira
authors: Hans F
authors: HF
authors: Dohmann
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells / Q000000981:diagnostic imaging*; D002545:Brain Ischemia / Q000150:complications* / Q000000981:diagnostic imaging*; D038524:Diffusion Magnetic Resonance Imaging; D006801:Humans; D007269:Injections, Intra-Arterial; D007963:Leukocytes, Mononuclear / Q000000981:diagnostic imaging*; D008297:Male; D011877:Radionuclide Imaging; D020521:Stroke / Q000150:complications* / Q000000981:diagnostic imaging*; D014182:Transplantation, Autologous