Determination of the antioxidant capacity of active food packagings by in situ gas-phase hydroxyl radical generation and high-performance liquid chromatography-fluorescence detection.
An experimental laboratory-made assembly to determine for the first time the antioxidant capacity with respect to hydroxyl (OH*) radicals of several new active packagings directly in the materials has been developed. Gas-phase OH* radicals are generated by UV-light irradiation of an aqueous H(2)O(2) aerosol. After on-line reaction with up to eight parallel test samples, remaining OH* is quantitatively trapped by a salicylic acid solution, and antioxidant capacity is indirectly assessed by HPLC-fluorescence determination of the high sensitive 2,5-dihydroxybenzoic acid formed. Several natural essential oils as well as active plastic films including in their formulation such oils have been subjected to oxidation. Polymers containing clove and oregano were the most efficient ones (up to 7.2 and 4.7 times, respectively, more antioxidant than blanks), whereas rosemary, citronella and propolis showed average efficiency. On the other hand, active materials containing ferulic acid, quercetin, catechin and thymol, as well as commercial active bags with ethylene-absorption properties, showed limited or none antioxidant protection. Experimental results and full details about experimental assembly are given.
Pezo
Davinson
D
Department of Analytical Chemistry, Aragon Institute of Engineering Research I3A, CPS-University of Zaragoza, Torres Quevedo Building, María de Luna St. 3, E-50018 Zaragoza, Spain.
Salafranca
Jesús
J
Nerín
Cristina
C
D000975:Antioxidants; D002851:Chromatography, High Pressure Liquid; D005453:Fluorescence; D018857:Food Packaging; D062385:Hydroxybenzoates / Q000032:analysis*; D017665:Hydroxyl Radical / Q000032:analysis*; D009822:Oils, Volatile / Q000737:chemistry; D010084:Oxidation-Reduction; D020156:Salicylic Acid / Q000737:chemistry
D000975:Antioxidants; D062385:Hydroxybenzoates; D009822:Oils, Volatile; D017665:Hydroxyl Radical; D020156:Salicylic Acid