title: [Generation of cell culture high-yield recombinant H3N2 subtype swine influenza vaccine candidate by reverse genetics].
abstract: High-yield H3N2 subtype swine influenza virus for large-scale vaccine production in cell culture was generated by reverse genetics. The rescued H3N2 (rH3N2) candidate virus contained hemagglutinin (HA) and neuraminidase (NA) genes derived from a field isolate A/Swine/Henan/S4/01 (H3N2), PB2 gene from A/PR/8/34, and the other five internal genes from A/Goose/Dalian/3/01 (H9N2). The rH3N2 virus titer in MDCK cell culture were measured by hemagglutination assay and the maximum virus titre of 1:512 hemagglutination unit was obtained after infection of MDCK cell for 60 h. The results of the present study indicated that rH3N2 virus was suitable for growth in MDCK cell culture and is feasible to be used for the production of cell grown influenza vaccine.
authors: National Key Laboratory of Veterinary Biotechnology and Animal Influenza Laboratory of the Ministry of Agriculture, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors: Tao
authors: T
authors: Yang
authors: Ming
authors: M
authors: Liu
authors: Chun-Guo
authors: CG
authors: Liu
authors: Yun
authors: Y
authors: Zhang
authors: Da-Fei
authors: DF
authors: Liu
authors: Hao
authors: H
authors: Chen
authors: Guang-Zhi
authors: GZ
authors: Tong
mesh_terms: D000818:Animals; D002460:Cell Line; D004285:Dogs; D006386:Hemagglutination Tests; D053122:Influenza A Virus, H3N2 Subtype / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D007252:Influenza Vaccines; D010957:Plasmids; D014776:Virus Cultivation
chemical_list: D007252:Influenza Vaccines