Crystallization and X-ray diffraction analysis of a novel immune-type receptor from Ictalurus punctatus and phasing by selenium anomalous dispersion methods.
X-ray diffraction data from crystals of a novel immune-type receptor (NITR10 from the catfish Ictalurus punctatus) were collected to 1.65 A resolution and reduced to the primitive hexagonal lattice. Native and selenomethionine derivatives of NITR10 crystallized under different conditions yielded P3(1)21 crystals. SeMet NITR10 was phased to a correlation coefficient of 0.77 by SAD methods and experimental electron-density maps were calculated to 1.65 A. Five NITR10 molecules are predicted to be present in the asymmetric unit based on the Matthews coefficient.
Ostrov
David A
DA
Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, 1600 SW Archer Road, Gainesville, FL 32610, USA. ostroda@pathology.ufl.edu
Hernández Prada
José A
JA
Haire
Robert N
RN
Cannon
John P
JP
Magis
Andrew T
AT
Bailey
Kate
K
Litman
Gary W
GW
D000818:Animals; D003460:Crystallization; D007059:Ictaluridae / Q000378:metabolism*; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D011971:Receptors, Immunologic / Q000737:chemistry* / Q000378:metabolism*; D012643:Selenium / Q000737:chemistry* / Q000378:metabolism*; D014961:X-Ray Diffraction / Q000379:methods*
D011971:Receptors, Immunologic; D012643:Selenium