Hepatic reperfusion in rats: a new model with portal arterialization in studying early ischemia-reperfusion injury.
Isolated liver perfusion has been used to evaluate the beneficial effects of several agents. In the present study, we developed a model using a recipient rat to reperfuse harvested livers in an ex situ, in vivo recirculating system. A total of 25 reperfusion procedures using adult male Wistar rats as donors and recipients were done. The preservation of the livers was performed with University of Wisconsin solution for 6 hours. Thereafter, the liver was reperfused with blood from another rat. We believe that the model presented herein offers an alternative method to evaluate early hepatocellular damage or hepatic microcirculation.
Pinto Kruel
C R
CR
Division of Gastroenterology, Post-Graduate Program in Gastroenterology, Universidade Federal do Rio Grande do Sul/Hospital de Clínicas de Porto Alegre, Brazil.
Scherer de Fraga
R
R
Dal Molin
S
S
Mota
S M
SM
Gasperin
G
G
Cerski
C T S
CT
de Oliveira
J R
JR
Alvares-da-Silva
M R
MR
D000818:Animals; D004195:Disease Models, Animal; D008102:Liver Circulation; D008297:Male; D008954:Models, Biological; D011168:Portal System / Q000473:pathology / Q000502:physiology* / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion / Q000379:methods*; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology*