Kinetics of C. elegans DcpS cap hydrolysis studied by fluorescence spectroscopy.
DcpS (scavenger decapping enzyme) from nematode C. elegans readily hydrolyzes both monomethyl- and trimethylguanosine cap analogues. The reaction was followed fluorimetrically. The marked increase of fluorescence intensity after the cleavage of pyrophosphate bond in dinucleotides was used to determine K(m) and V(max)values. Kinetic parameters were similar for both classes of substrates and only slightly dependent on pH. The hydrolysis was strongly inhibited by methylene cap analogues (m(7)Gp(CH(2))ppG and m(7)Gpp(CH(2))pG) and less potently by ARCA (m(7,3' O)GpppG).
Wierzchowski
J
J
Department of Biophysics, University of Warmia and Mazury, 4 Oczapowskiego, Olsztyn, Poland. jacek.wie@uwm.edu.pl
Pietrzak
M
M
Stepinski
J
J
Jemielity
J
J
Kalek
M
M
Bojarska
E
E
Jankowska-Anyszka
M
M
Davis
R E
RE
Darzynkiewicz
E
E
D000818:Animals; D029742:Caenorhabditis elegans Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry*; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D011755:Pyrophosphatases / Q000037:antagonists & inhibitors / Q000737:chemistry*; D012314:RNA Cap Analogs / Q000737:chemistry* / Q000494:pharmacology; D013050:Spectrometry, Fluorescence
D029742:Caenorhabditis elegans Proteins; D012314:RNA Cap Analogs; D006147:Guanine; C008450:7-methylguanine; D011755:Pyrophosphatases; C509343:dcs-1 protein, C elegans