Thermodynamic analysis of quadruplex DNA-drug interaction.
This work studies the binding properties of distamycin and its carbamoyl analog, containing four pyrrole units, with the [d(TGGGGT)](4) quadruplex by means of isothermal titration calorimetry (ITC). Analysis of the ITC data reveals that drug/quadruplex binding stoichiometry is 1:1 for both interactions and that distamycin analog gives approximately a 10-fold increase in the quadruplex affinity.
Pagano
Bruno
B
Dipartimento di Scienze Farmaceutiche, Università di Salerno, Fisciano SA, Italy.
Mattia
Carlo A
CA
Virno
Ada
A
Randazzo
Antonio
A
Mayol
Luciano
L
Giancola
Concetta
C
D002151:Calorimetry; D004214:Distamycins / Q000737:chemistry*; D004347:Drug Interactions; D054856:G-Quadruplexes; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012996:Solutions; D013816:Thermodynamics
D004214:Distamycins; D012996:Solutions; C030000:stallimycin