title: Inhibition of Listeria monocytogenes in dairy products using the bacteriocin-like peptide cerein 8A.
abstract: The efficacy of the antimicrobial peptide cerein 8A to control the development of Listeria monocytogenes in milk and soft cheese was investigated. The addition of 160 AU ml(-1) cerein 8A to UHT milk resulted in a decrease of 3 log cycles in viable cells within the 14-day period at 4 degrees C. The viable counts of L. monocytogenes in pasteurized milk samples containing cerein 8A was lower than those observed in controls without bacteriocin. Addition of cerein 8A to Minas-type soft cheese caused a delay in the start of exponential growth phase, although similar counts were observed after day 6. When cerein 8A was used to control cheese surface contamination by L. monocytogenes, a decrease of 2 log cycles in viable counts of cerein-treated samples was observed during 30 days at 4 degrees C. This antimicrobial peptide shows potential use as a biopreservative for application in dairy products.
authors: Laboratório de Bioquímica e Microbiologia Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 91501-970 Porto Alegre, Brazil.
authors: Delmar
authors: D
authors: Bizani
authors: Juliana A C
authors: JA
authors: Morrissy
authors: Ana P M
authors: AP
authors: Dominguez
authors: Adriano
authors: A
authors: Brandelli
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001409:Bacillus cereus / Q000378:metabolism*; D001430:Bacteriocins / Q000494:pharmacology*; D002611:Cheese / Q000382:microbiology; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D003611:Dairy Products / Q000382:microbiology*; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control; D005519:Food Preservation / Q000379:methods; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C505873:cerein 8A, Bacillus cereus