[Study on the pathogens correlated to sexually transmitted diseases in 285 pre-pubertal girls with vulvovaginitis in Beijing].
OBJECTIVE
To study the relationship between vulvovaginitis in pre-pubertal girls and pathogens as Chlamydia trachomatis (Ct), N. gonorrhoeae (Ng), Mycoplasma, Ureaplasma urealyticum (Uu), Mycoplasma hominis (Mh), M. genitalium (Mg), M. fermentans (Mf) and M. penetrans (Mpe), as well as to find out the proportion of mycoplasma which is correlated to sexually transmitted diseases (STD) and AIDS. METHODS Vulvae swab specimens from 285 pre-pubertal girls with vulvovaginitis (case group) and 128 healthy girls (control group) were collected and detected by nested polymerase chain reaction (nPCR) to identify the existence of pathogens as Ct, Ng, Uu, Mh, Mg, Mf and Mpe. nPCR with both high specificity and sensitivity, would not be influenced by the amount of pathogens in specimens or inactivated during the process of storage or transportation.


RESULTS
The rate of detection on pathogens was 59.65% in the 285 specimens from case group including 'one kind of pathogen in one specimen' as 37.54% and 'two kinds' as 16.84% and 'three kinds' as 5.26%. However, in the 128 specimens from control group, the detectable rate of pathogen was 6.25%. Relationships were found between Ng (P < 0.01), Ct (P < 0.01), Uu (P < 0.01), Mg (P < 0.01), Mf (P < 0.05), Mpe (P < 0.01) and vulvovaginitis in pre-pubertal girls. In control group the pathogens were detected from 7 specimens including 5 Uu and 2 Mh.


CONCLUSIONS
Some of the pathogens were correlated to STD and were important in causing vulvovaginitis in pre-pubertal girls. Vulvovaginitis might have been caused by more than one kind of pathogen in pre-pubertal girls. The locations of Mg, Mf and Ng in outer genital tracts were correlated to seasonal change. Macrolide seemed to be quite effective clinically in treating urogenital tract infection caused by mycoplasma and Ct.
Liu
Xiao-Yan
XY
Capital Institute of Pediatrics, Beijing, China.
Sun
Hong-Mei
HM
Feng
Yan-Ling
YL
Hu
Jin
J
Zhao
Han-Qing
HQ
Zhang
Li-Ya
LY
D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002681:China; D002690:Chlamydia Infections / Q000150:complications; D002692:Chlamydia trachomatis / Q000302:isolation & purification; D005260:Female; D006069:Gonorrhea / Q000150:complications; D006801:Humans; D009174:Mycoplasma / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000150:complications; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification; D012621:Seasons; D012680:Sensitivity and Specificity; D012749:Sexually Transmitted Diseases / Q000150:complications / Q000382:microbiology*; D016869:Ureaplasma Infections / Q000150:complications; D016990:Ureaplasma urealyticum / Q000302:isolation & purification; D014848:Vulvovaginitis / Q000209:etiology / Q000382:microbiology*