The canine hookworm genome: analysis and classification of Ancylostoma caninum survey sequences.
Hookworms infect nearly a billion people. The Ancylostoma caninum hookworm of canids is a model for studying human infections and information from its genome coupled with functional genomics and proteomics can accelerate progress towards hookworm control. As a step towards a full-scale A. caninum genome project, we generated 104,000 genome survey sequences (GSSs) and determined the genome size of the canine hookworm. GSSs assembled into 57.6 Mb of unique sequence from a genome that we estimate by flow cytometry of isolated nuclei to be 347 +/- 1.2 Mb, substantially larger than other Rhabditina species. Gene finding identified 5538 genes in the GSS assembly, for a total of 9113 non-redundant A. caninum genes when EST sequences are also considered. Functional classifications of many of the 70% of genes with homology to genes in other species are provided based on gene ontology and KEGG associations and secreted and membrane-bound proteins are also identified.
Abubucker
Sahar
S
Department of Genetics, Genome Sequencing Center, Washington University School of Medicine, St Louis, MO 63108, USA.
Martin
John
J
Yin
Yong
Y
Fulton
Lucinda
L
Yang
Shiaw-Pyng
SP
Hallsworth-Pepin
Kym
K
Johnston
J Spencer
JS
Hawdon
John
J
McCarter
James P
JP
Wilson
Richard K
RK
Mitreva
Makedonka
M
D000722:Ancylostoma / Q000235:genetics*; D000818:Animals; D020224:Expressed Sequence Tags; D049751:Genome, Helminth; D023281:Genomics; D015801:Helminth Proteins / Q000235:genetics* / Q000502:physiology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D015801:Helminth Proteins