Embryotoxicity of serum from women smoking cocaine base paste (CBP).
OBJECTIVE
Using a mouse embryo bioassay, we examined the embryotoxic properties of serum obtained from eight women who had ingested cocaine base paste (CBP) for a period of 1-3 years.


METHODS
Two-cell stage mouse embryos were cultured in the presence of serum extracted from eight CBP-smoking or control women. After 48h, embryos were evaluated to determine their developmental stage and then processed by Tarkoswki's technique to determine the proportion of embryos with micronuclei, in order to establish the number of micronuclei/embryo.


RESULTS
Serum from CBP patients diminished the percentage of embryos progressing to the compacted morula stage, while increasing the proportion of embryos with micronuclei and the corresponding micronuclei/embryo ratio.


CONCLUSIONS
These findings provide the first experimental evidence of embryotoxic compounds in the serum of CBP-smoking women. This study highlights the reproductive risk of chronically ingested CBP and demonstrates the need for further studies.
Del Valle
Luis J
LJ
Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Peru.
Pella
Ricardo
R
Mercedes
Azucena
A
Velasquez
Luis A
LA
Orihuela
Pedro A
PA
D000328:Adult; D000818:Animals; D016022:Case-Control Studies; D002869:Chromosome Aberrations / Q000139:chemically induced*; D003042:Cocaine / Q000633:toxicity*; D019970:Cocaine-Related Disorders / Q000097:blood*; D004195:Disease Models, Animal; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D006801:Humans; D051379:Mice; D011247:Pregnancy; D013723:Teratogens
D013723:Teratogens; D003042:Cocaine