Turmeric-based diet can delay apoptosis without modulating NF-kappaB in unilateral ureteral obstruction in rats.
The unilateral ureteral obstruction (UUO) model of renal injury in rat is characterized by nuclear factor kappaB (NF-kappaB) activation and tumour necrosis factor alpha (TNF-alpha) production, which induces apoptosis via activation of caspase 8 resulting in cell death. Curcumin, the major component found in turmeric spice, has been reported to provide protection against fibrosis and apoptosis elicited by UUO. This study examined the effects of a turmeric-based diet (5% w/w) on the apoptotic pathway induced by UUO in rats after 30 days of ligation. Administration of a turmeric-based diet demonstrated a significant decrease (P<0.05) in mRNA expression of TNF-alpha and caspase 8, but not NF-kappaB, expression, which may contribute to the protective role of the turmeric-based diet. We conclude that a turmeric-based diet can delay apoptosis without modulating NF-kappaB, so as not to sensitize the mesangial cells to the apoptotic stimuli.
Hashem
Reem M
RM
Department of Biochemistry, Faculty of Pharmacy, Beni-suief University, Egypt. drreem30@yahoo.com
Soliman
Hala M
HM
Shaapan
Sahar F
SF
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001806:Blood Urea Nitrogen; D053181:Caspase 8 / Q000235:genetics / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D030024:Curcuma / Q000737:chemistry*; D004032:Diet; D004195:Disease Models, Animal; D004587:Electrophoresis, Agar Gel; D024022:Fibrillar Collagens / Q000378:metabolism; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014517:Ureteral Obstruction / Q000178:diet therapy* / Q000235:genetics / Q000378:metabolism
D024022:Fibrillar Collagens; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D003404:Creatinine; D053181:Caspase 8