Urinary markers of altered collagen metabolism in fibromyalgia patients.
OBJECTIVE
To assess the metabolism of collagen in fibromyalgia (FM) patients, and to compare the occurrence of collagen metabolism markers to the severity of FM symptoms.


METHODS
Morning urine was collected from 27 FM women fulfilling the American College of Rheumatology (ACR) criteria for FM, and from seven controls. FM patients completed the Fibromyalgia Impact Questionnaire (FIQ). Bone mineral density (BMD), isokinetic muscle strength in knee and elbow, and hand-grip strength were measured. Urinary concentrations of collagen type I cross-linked C-telopeptide (CTX-I) and collagen type II cross-linked C-telopeptide (CTX-II) were determined by enzyme-linked immunosorbent assay (ELISA). Pyridinoline (Pyd) and deoxypyridinoline (Dpd) were determined by liquid chromatography, and hydroxyproline (Hyp) by spectrophotometry. All concentration data were normalized to creatinine.


RESULTS
Mean values in the FM group and the control group, respectively, were: urinary CTX-I 246.8 and 337.5 microg/mmol (p = 0.060); CTX-II 110.4 and 185.1 ng/mmol (p = 0.035); Pyd 56.1 and 52.3 nmol/mmol (NS); Dpd 15.1 and 14.0 nmol/mmol (NS); Pyd : Dpd ratio 4.05 and 3.96 (NS); Hyp 26.1 and 21.1 micromol/mmol (NS). Significant inverse correlations were seen between CTX-I and the intensity of fatigue, and between CTX-II and anxiety. An inverse correlation between CTX-I and muscle strength was apparent, but relied on extreme values from one patient, and no significant correlation was found between CTX-I or CTX-II and tender points or BMD in the FM group.


CONCLUSIONS
Low urinary concentrations of CTX-II and CTX-I and normal levels of Pyd and Dpd were found in FM, but their relationship to the intensity of FM symptoms was unclear.
Ribel-Madsen
S
S
The Parker Institute, Department of Rheumatology, Frederiksberg Hospital, Capital Region of Denmark, DK-2000 Frederiksberg, Denmark. soren.ribel.madsen@frh.regionh.dk
Christgau
S
S
Gronemann
S T
ST
Bartels
E M
EM
Danneskiold-Samsøe
B
B
Bliddal
H
H
D000328:Adult; D015415:Biomarkers / Q000652:urine*; D015519:Bone Density; D002853:Chromatography, Liquid; D024042:Collagen Type I / Q000652:urine*; D024043:Collagen Type II / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005356:Fibromyalgia / Q000652:urine*; D006801:Humans; D010455:Peptides / Q000652:urine*; D011379:Prognosis; D012720:Severity of Illness Index; D013053:Spectrophotometry; D011795:Surveys and Questionnaires
D015415:Biomarkers; D024042:Collagen Type I; D024043:Collagen Type II; D010455:Peptides; C052929:collagen type I trimeric cross-linked peptide