title: Long-range action of Nodal requires interaction with GDF1.
abstract: GDF1 (growth/differentiation factor 1), a Vg1-related member of the transforming growth factor-beta superfamily, is required for left-right patterning in the mouse, but the precise function of GDF1 has remained largely unknown. In contrast to previous observations, we now show that GDF1 itself is not an effective ligand but rather functions as a coligand for Nodal. GDF1 directly interacts with Nodal and thereby greatly increases its specific activity. Gdf1 expression in the node was found necessary and sufficient for initiation of asymmetric Nodal expression in the lateral plate of mouse embryos. Coexpression of GDF1 with Nodal in frog embryos increased the range of the Nodal signal. Introduction of Nodal alone into the lateral plate of Gdf1 knockout mouse embryos did not induce Lefty1 expression at the midline, whereas introduction of both Nodal and GDF1 did, showing that GDF1 is required for long-range Nodal signaling from the lateral plate to the midline. These results suggest that GDF1 regulates the activity and signaling range of Nodal through direct interaction.
authors: Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation (JST), Suita, Osaka 565-0871, Japan.
authors: Chinatsu
authors: C
authors: Tanaka
authors: Rui
authors: R
authors: Sakuma
authors: Tetsuya
authors: T
authors: Nakamura
authors: Hiroshi
authors: H
authors: Hamada
authors: Yukio
authors: Y
authors: Saijoh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017077:Culture Media, Conditioned; D055452:Growth Differentiation Factor 1; D047468:Immunoprecipitation; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D055457:Nodal Protein; D011485:Protein Binding; D016212:Transforming Growth Factor beta / Q000378:metabolism / Q000502:physiology*; D014981:Xenopus
chemical_list: D017077:Culture Media, Conditioned; C525978:Gdf1 protein, mouse; D055452:Growth Differentiation Factor 1; D036341:Intercellular Signaling Peptides and Proteins; D055457:Nodal Protein; C526008:Nodal protein, mouse; D016212:Transforming Growth Factor beta