Recognition profiles of microsporidian Encephalitozoon cuniculi polar tube protein 1 with human immunoglobulin M antibodies.
Microsporidian Encephalitozoon cuniculi has a unique organelle called a polar tube (PT), the extrusion of which is absolutely required to invade a host cell. We recently detected anti-E. cuniculi PT immunoglobulin (Ig) M antibodies in sera from many healthy individuals. The present one-dimensional (1-D) immunoblot analysis predominantly detected a band at 52 kDa in all of the examined human sera with anti-PT IgM. The use of mouse monoclonal antibody confirmed that the 52-kDa band detected in 1-D immunoblots was an antigen derived from the PT, which represents a glycoprotein nature. In addition, from changes in the immunoreactivity of the 52-kDa band before and after treatment with NaOH, we determined that the 24 human serum samples with anti-PT IgM activities could be roughly grouped into three types: (i) sera containing antibodies against only a saccharic determinant (n=3); (ii) sera containing antibodies against only a proteinic determinant (n=11); and (iii) sera showing dual recognition of saccharic and proteinic determinants (n=10). Further two-dimensional (2-D) immunoblot analysis followed by proteomic analysis confirmed that human sera with anti-PT IgM reacted with E. cuniculi polar tube protein 1 (PTP1). Such circulating IgM antibodies may be important in the first line of defence against E. cuniculi infection.
Furuya
K
K
Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan. kfuruya@nih.go.jp
Omura
M
M
Kudo
S
S
Sugiura
W
W
Azuma
H
H
D000908:Antibodies, Fungal / Q000097:blood* / Q000276:immunology; D003208:Concanavalin A / Q000378:metabolism; D009670:Encephalitozoon cuniculi / Q000276:immunology*; D016890:Encephalitozoonosis / Q000276:immunology* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005656:Fungal Proteins / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood* / Q000276:immunology; D016814:Microsporidia / Q000276:immunology; D040901:Proteomics
D000908:Antibodies, Fungal; D005656:Fungal Proteins; D007075:Immunoglobulin M; D003208:Concanavalin A