Hybridization biosensor using 2,9-dimethyl-1,10-phenantroline cobalt as electrochemical indicator for detection of hepatitis B virus DNA.
A label-free biosensor for the detection of oligonucleotides related to hepatitis B virus sequence via the interactions of DNA with redox-active complex, 2,9-dimethyl-1,10-phenantroline cobalt [Co(dmp)(H2O)(NO3)2] is described. The study was carried out by the hybridization of 21-mer probe DNA modified on glassy carbon electrode (GCE) with target DNA, and [Co(dmp)(H2O)(NO3)2] whose sizes are comparable to those of the small groove of native double-helix DNA was used as an electrochemical indicator. Electrochemical detection was performed by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) over the potential range where the [Co(dmp)(H2O)(NO3)2] was active. Under the optimum conditions, the electrical signal had a linear relationship with the concentration of target DNA ranging from 3.96 x 10(-7) to 1.32 x 10(-6) M, and the detection limit was 1.94 x 10(-8) M (S/N=3). The biosensor has good selectivity by detecting the three-base mismatch sequence ssDNA.
Ding
Caifeng
C
Key Laboratory of Eco-chemical Engineering, Ministry of Education; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Zhao
Fei
F
Zhang
Meiling
M
Zhang
Shusheng
S
D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D003035:Cobalt / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D006515:Hepatitis B virus; D006860:Hydrogen Bonding; D009566:Nitrates / Q000737:chemistry; D010618:Phenanthrolines / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D009566:Nitrates; D010618:Phenanthrolines; D014867:Water; D003035:Cobalt