title: Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin.
abstract: HA3, a 70 kDa haemagglutinating protein, is a precursor form of HA3a and HA3b, the subcomponents of Clostridium botulinum type C 16S progenitor toxin. In this report, recombinant HA3 protein was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.6 A resolution and the crystal belonged to the hexagonal space group P6(3). Matthews coefficient and self-rotation function calculations indicate that there is probably one molecule of HA3 in the asymmetric unit. A search for heavy-atom derivatives has been undertaken.
authors: Department of Applied Biological Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
authors: Toshio
authors: T
authors: Nakamura
authors: Takashi
authors: T
authors: Tonozuka
authors: Mao
authors: M
authors: Kotani
authors: Kanae
authors: K
authors: Obata
authors: Keiji
authors: K
authors: Oguma
authors: Atsushi
authors: A
authors: Nishikawa
mesh_terms: D001905:Botulinum Toxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003014:Clostridium botulinum / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003460:Crystallization; D014961:X-Ray Diffraction
chemical_list: D001905:Botulinum Toxins; C096324:botulinum toxin type C