title: Flow cytometric assessment of anti-neuronal antibodies in central nervous system involvement of systemic lupus erythematosus and other autoimmune diseases.
abstract: The objective of this study is to evaluate the association between anti-neuronal antibody (anti-NA) and central nervous system (CNS) manifestations of systemic lupus erythematosus (SLE) and other rheumatic diseases using a flow cytometric method. Anti-NA was measured by flow cytometry in serum and cerebrospinal fluid (CSF) samples from patients with SLE (n=44 for serum, n=17 for CSF), other rheumatic diseases (n=64 for serum, n=21 for CSF) and from healthy controls (n=65 for serum, n=18 for CSF). Serum anti-NA was more frequently observed in SLE (31.8%, 14/44) than in other rheumatic diseases (4.7%, 3/64, P<0.001) or in healthy controls (0%, 0/65, P<0.00001). In SLE patients, the frequency of serum anti-NA was significantly higher in CNS-SLE (76.5%, 13/17) than in non CNS-SLE (3.7%, 1/27, P<0.000001). CSF anti-NA was detected in 88.2% (15/17) of CNS-SLE and was more frequently detected in CNS-SLE (15/17, 88.2%) than in other rheumatic diseases with CNS involvement (1/21, 4.8%, P<0.000001) or in healthy controls (0/18, P<0.000001). In conclusion, serum anti-NA was more frequently found in CNS-SLE than in non CNS-SLE, other rheumatic diseases or in healthy controls. The frequency of CSF anti-NA in CNS-SLE was significantly higher than in other rheumatic diseases with CNS involvement or in healthy controls.
authors: Division of Rheumatology, Department of Internal Medicine, Medical Research Center, Seoul National University, College of Medicine, Seoul, Republic of Korea.
authors: E H
authors: EH
authors: Kang
authors: G Q
authors: GQ
authors: Shen
authors: R
authors: R
authors: Morris
authors: A
authors: A
authors: Metzger
authors: E Y
authors: EY
authors: Lee
authors: Y J
authors: YJ
authors: Lee
authors: E B
authors: EB
authors: Lee
authors: Y W
authors: YW
authors: Song
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000097:blood / Q000134:cerebrospinal fluid; D045744:Cell Line, Tumor; D002561:Cerebrovascular Disorders / Q000276:immunology; D003221:Confusion / Q000276:immunology; D004827:Epilepsy / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D020773:Headache Disorders / Q000276:immunology; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000150:complications / Q000276:immunology*; D020945:Lupus Vasculitis, Central Nervous System / Q000150:complications / Q000276:immunology*; D008297:Male; D008582:Meningitis, Aseptic / Q000276:immunology; D008875:Middle Aged; D009474:Neurons / Q000276:immunology*; D011618:Psychotic Disorders / Q000276:immunology; D012216:Rheumatic Diseases / Q000150:complications / Q000276:immunology*; D015854:Up-Regulation
chemical_list: D001323:Autoantibodies