An optimal embryo transfer condition for the effective production of DBA/2J mice.
The DBA/2J mouse strain is a standard laboratory strain that is widely used for biomedical research. This strain, however, suffers from poor reproductive performance. In addition, the conditions for reliable embryo transfer (ET) of this strain have not been elucidated. The intention of this study was to determine the optimal number of embryos for transfer that allow the effective production of DBA/2J offspring. In the experiment, 7 to 15 embryos per oviduct were transferred into pseudopregnant ICR females. A relatively high success rate for pup production was observed when a large number of DBA/2J embryos (30 embryos per female) were transferred. This result shows that the ET efficiency of the DBA/2J strain can be improved by increasing the number of transferred embryos.
Kaneda
Hideki
H
Functional Genomics Research Group, RIKEN Genomic Sciences Center, Ibaraki, Japan.
Taguma
Kyuichi
K
Suzuki
Chigusa
C
Ozaki
Ai
A
Nakamura
Chika
C
Hachisu
Akiko
A
Kobayashi
Kimio
K
Wakana
Shigeharu
S
Shiroishi
Toshihiko
T
D000818:Animals; D004624:Embryo Transfer / Q000379:methods / Q000662:veterinary*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA / Q000502:physiology*; D008813:Mice, Inbred ICR