IL-12Bpro and GSTP1 polymorphisms in association with silicosis.
To assess the role of IL-12Bpro and GSTP1 polymorphisms on induced interleukin (IL)-12p40 production in respect to silicosis development, we examined their distribution in 63 silicosis patients and 165 healthy donors. Decreased frequencies of IL-12Bpro-2 allele (42% vs 62.5%, P = 0.0042) and genotype 22 (19% vs 40%, P = 0.02) in patients compared with exposed healthy donors suggested their protective role in silicosis susceptibility. The GSTP1 A/G polymorphism was also associated with silicosis susceptibility. The allele G and genotype GG were overrepresented among patients than among healthy men (36% vs 23%, P = 0.013; 14% vs 2%, P(c) = 0.012). Also, patients' peripheral blood mononuclear cell (PBMC) produced higher IL-12p40 than healthy donors' depending on both IL-12Bpro and GSTP1 polymorphisms. In conclusion, homozygosity of high producer IL-12p40 genotype IL-12Bpro-11 and GSTP1-GG had the highest genetic risk of silicosis development in Bulgarian miners.
Stanilova
S
S
Department of Molecular Biology, Immunology & Medical Genetics, Faculty of Medicine, Trakia University, Stara Zagora, Bulgaria. stanilova@mf.uni-sz.bg
Miteva
L
L
Prakova
G
G
D000328:Adult; D000368:Aged; D000483:Alleles; D002031:Bulgaria; D003032:Coal Mining; D005260:Female; D020022:Genetic Predisposition to Disease; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D006801:Humans; D053762:Interleukin-12 Subunit p40 / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012829:Silicosis / Q000235:genetics*
D053762:Interleukin-12 Subunit p40; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi