Heterologous expression of the pneumococcal serotype 14 polysaccharide in Lactococcus lactis requires lactococcal epsABC regulatory genes.
The pneumococcal serotype 14 polysaccharide was produced in Lactococcus lactis by coexpressing pneumococcal polysaccharide type 14-specific genes (cpsFGHIJKL(14)) with the lactococcal regulatory and priming glucosyltransferase-encoding genes specific for B40 polysaccharide (epsABCD(B40)). The polysaccharide produced by Lactococcus was secreted in the medium, simplifying downstream processing and polysaccharide isolation from culture broth.
Nierop Groot
Masja N
MN
NIZO food research, Kernhemseweg 2, P.O. Box 20, 6710 BA Ede, The Netherlands.
Godefrooij
Jeroen
J
Kleerebezem
Michiel
M
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial; D005964:Glucosyltransferases