[Study on HPLC fingerprint of water-soluble constituents of Carthamus tinctorius].
OBJECTIVE
To establish the HPLC-fingerprint of the water-soluble constituents of Carthamus tinctorius.


METHODS
18 samples of Carthamus tinctorius from different producing areas were determined by Agilent 1100 DAD-HPLC under the chromatographic conditions: column by SinoChrom ODS-BP (250 mm x 4.6 mm, 5 microm), methanol-0.7% H3PO4 water with gradient elution, column temperature 30 degrees C, flow rate by 1 ml/min, wavelength 280 nm, and inject volume 20 microl.


RESULTS
The HPLC-fingerprint of the water-soluble constituents of Cartharnus tinctorius was established on the basis of 10 bitch of drugs from Xinjiang according to SPSS analysis.


CONCLUSIONS
A reliable method is provided for the quality identification of Carthamus tinctorius.
Tang
Xu-li
XL
Ocean University of China, Qingdao 266003, China.
Liu
Jing
J
Li
Guo-qiang
GQ
Zhong
Hui-min
HM
Qiu
Pei-ju
PJ
Zhang
Jing
J
D031187:Carthamus tinctorius / Q000737:chemistry* / Q000145:classification; D002599:Chalcone / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000592:standards; D035264:Flowers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D002599:Chalcone