Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster.
The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.
Ni
Jian-Quan
JQ
Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02175, USA.
Markstein
Michele
M
Binari
Richard
R
Pfeiffer
Barret
B
Liu
Lu-Ping
LP
Villalta
Christians
C
Booker
Matthew
M
Perkins
Lizabeth
L
Perrimon
Norbert
N
D000818:Animals; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D034622:RNA Interference
D029721:Drosophila Proteins