title: First molecular detection of Borrelia afzelii in clinical samples in Korea.
abstract: Borrelia afzelii nucleic acids were detected in the sera of febrile disease patients by a nested PCR that targeted the rrf (5S)-rrl (23S) spacer of B. burgdorferi sensu lato. The B. afzelii-specific DNA fragment was detected in 8 out of 283 sera which were proven to have immunoglobulin G or M antibodies against B. burgdorferi antigens through IFA. The results were further confirmed through restriction fragment length polymorphism and sequencing analysis of the DNA fragments. The results indicated for the first time that Lyme borreliosis is prevalent in Korea.
authors: Department of Microbiology, College of Medicine, Konkuk University, Seoul, Republic of Korea.
authors: Yeon-Joo
authors: YJ
authors: Choi
authors: Seung-Hoon
authors: SH
authors: Han
authors: Jin-Mi
authors: JM
authors: Park
authors: Kyung-Min
authors: KM
authors: Lee
authors: Eun-Mi
authors: EM
authors: Lee
authors: Seung-Hyun
authors: SH
authors: Lee
authors: Hyeon-Je
authors: HJ
authors: Song
authors: Young-Sang
authors: YS
authors: Koh
authors: Keun-Wha
authors: KW
authors: Lee
authors: Won-Jong
authors: WJ
authors: Jang
authors: Kyung-Hee
authors: KH
authors: Park
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood; D000954:Antigens, Surface / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001428:Bacterial Vaccines / Q000235:genetics; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000097:blood / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000097:blood / Q000737:chemistry / Q000235:genetics; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007723:Korea; D008074:Lipoproteins / Q000235:genetics; D008193:Lyme Disease / Q000175:diagnosis* / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D044967:Serum / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008074:Lipoproteins; C072045:OspA protein