title: Anacardic acids and ferric ion chelation.
abstract: 6-Pentadeca(e)nylsalicylic acids isolated from the cashew Anacardium occidentale L. (Anacardiaceae), commonly known as anacardic acids, inhibited the linoleic acid peroxidation catalyzed by soybean lipoxygenase-1 (EC 1.13.11.12, type 1) competitively without prooxidant effects. Their parent compound, salicylic acid, did not have this inhibitory activity up to 800 pm, indicating that the pentadeca(e)nyl group is an essential element to elicit the activity. The inhibition is attributed to its ability to chelate iron in the enzyme. Thus, anacardic acids chelate iron in the active site of the enzyme and then the hydrophobic tail portion slowly begins to interact with the hydrophobic domain close to the active site. Formation of the anacardic acids-ferric ion complex was detected in the ratio of 2:1 as the base peak in the negative ion electrospray ionization mass spectrometry. Hence, anacardic acids inhibit both Eox and Ered forms.
authors: School of Material Sciences, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa 923-1292, Japan. tujimoto@jaist.ac.jp
authors: Kazuo
authors: K
authors: Tsujimoto
authors: Akio
authors: A
authors: Hayashi
authors: Tae Joung
authors: TJ
authors: Ha
authors: Isao
authors: I
authors: Kubo
mesh_terms: D048729:Anacardic Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D031021:Anacardium / Q000737:chemistry*; D007502:Iron Chelating Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007700:Kinetics; D008084:Lipoxygenase / Q000378:metabolism*; D016859:Lipoxygenase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D048729:Anacardic Acids; D007502:Iron Chelating Agents; D016859:Lipoxygenase Inhibitors; C101084:lipoxygenase L-1; D008084:Lipoxygenase