title: Genetic structure of the invasive pest Bemisia tabaci: evidence of limited but persistent genetic differentiation in glasshouse populations.
abstract: The geographic range of plant pests can be modified by the use of glasshouses. Bemisia tabaci, originating from warm to hot climates, has been shown to be a complex of distinct genetic groups with very limited gene flow. The genetic structure of this pest was studied in glasshouses in southern France, a region beyond the northern limit of its open-field development area in Europe. Seven microsatellite loci were scored in 22 populations sampled from various regions over 3 years. Two genetic groups were distinguished using a Bayesian clustering method and were assigned to the so-called biotypes B and Q using the gene sequence of cytochrome oxidase 1 (CO1). All but one population corresponded to biotype Q, even though only biotype B was previously reported. Despite the enclosed environment of glasshouses and their expected isolation due to low outdoor survival during the winter, only limited differentiation among biotype Q glasshouses was observed. A single sample site was notable for a decrease in expected heterozygosity and the mean number of alleles over the years. The lack of spatial genetic structure among biotype Q populations was indicative of a recent colonization event combined with large dispersal at all spatial scales. This migration pattern of biotype Q populations was further supported by additional CO1 sequences, since individuals from France, Asia and America exhibited 100% nucleotide identity. The evolution of genetic diversity observed in glasshouses in France is part of the worldwide invasion of biotype Q, which is discussed in light of human activities.
authors: INRA, LNPV, Unite de Pathologie Végetale, Domaine St Maurice, Montfavet cedex, France.
authors: A
authors: A
authors: Dalmon
authors: F
authors: F
authors: Halkett
authors: M
authors: M
authors: Granier
authors: H
authors: H
authors: Delatte
authors: M
authors: M
authors: Peterschmitt
mesh_terms: D000818:Animals; D001483:Base Sequence; D001499:Bayes Theorem; D016000:Cluster Analysis; D003710:Demography; D003576:Electron Transport Complex IV / Q000235:genetics; D019143:Evolution, Molecular; D005602:France; D051456:Gene Flow / Q000235:genetics; D040961:Genetic Drift; D014644:Genetic Variation; D005828:Genetics, Population; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006430:Hemiptera / Q000145:classification / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D003576:Electron Transport Complex IV