[The methylation locus and frequency pattern on p16 INK4a gene promoter CpG in epidermis of patients with psoriasis].
OBJECTIVE
To investigate the CpG methylation locus and frequency pattern on p16 INK4a gene promoter in epidermis of p16 INK4a methylated patients with psoriasis vulgaris.


METHODS
The DNA specimens were obtained from epidermal lesion of 50 plaque psoriatic patients. Methylation specific PCR and DNA sequencing were used to detect the frequency and locus of methylation in p16 INK4a gene promoter region.


RESULTS
Approximately 50% CpG was methylated in p16 INK4a methylated patients, methylation was found in specifical locus of p16 INK4a gene promoter.


CONCLUSIONS
The distinct methylation pattern is showed on the p16 INK4a gene promoter region in patients with psoriasis.
Chen
Min
M
Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, Jiangsu, 210042 PR China.
Cui
Pan-gen
PG
Yao
Xu
X
Cao
Yuan-hua
YH
Gong
Juan-qin
JQ
Li
An-sheng
AS
Chen
Zhi-qiang
ZQ
D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D018899:CpG Islands / Q000235:genetics*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation / Q000235:genetics*; D004817:Epidermis / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011565:Psoriasis / Q000235:genetics*; D016415:Sequence Alignment; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics; D055815:Young Adult
D019941:Cyclin-Dependent Kinase Inhibitor p16; D025803:Tumor Suppressor Protein p14ARF