title: Inhibitory effects of atrazine on Chlorella vulgaris as assessed by real-time polymerase chain reaction.
abstract: Atrazine, a highly toxic herbicide, is frequently detected in surface water because of its heavy application. Algae are among the aquatic organisms most susceptible to atrazine pollution in water. In the present study, the aquatic alga Chlorella vulgaris Beijerinck was chosen to assess the acute toxicity of atrazine (48-96 h) in terms of gene transcription and physiological changes. A real-time polymerase chain reaction (PCR) assay was used to quantify transcript levels of three photosystem genes in C. vulgaris. The diel patterns for regulation of the psaB (photosystem I reaction center protein subunit B), psbC (an integral membrane protein component of photosystem II), and rbcL (large subunit of ribulose-1,5-bisphosphate carboxylase oxygenase) gene transcripts were successfully quantified. Results showed that atrazine reduced the transcript abundances of three target genes and that the abundances decreased with increasing atrazine concentration. The determined smallest transcript levels of psaB, psbC, and rbcL, which occurred at the highest atrazine concentration tested (400 mug/L), were only 34.6, 34.6, and 8.1%, respectively, of the control sample value. Exposure to atrazine increased the level of malondialdehyde by 1.74-fold (the highest value) in C. vulgaris, suggesting potential oxidative damage to the alga. The activities of antioxidation enzymes (e.g., superoxide dismutase, peroxidase, and catalase) also increased markedly in the presence of atrazine, with maximum increases of 1.82-, 1.59-, and 2.31-fold, respectively. These elevated activities may help to alleviate the oxidative damage. Our results demonstrate that atrazine is highly toxic to this alga and that real-time PCR is an efficient technique for assessing the toxicity of xenobiotic compounds in algae.
authors: College of Biological and Environmental Engineering, Zheijiang University of Technology, Hangzhou, People's Republic of China.
authors: Haifeng
authors: H
authors: Qian
authors: G
authors: G
authors: Daniel Sheng
authors: Weiping
authors: W
authors: Liu
authors: Yingcong
authors: Y
authors: Lu
authors: Zhenghai
authors: Z
authors: Liu
authors: Zhengwei
authors: Z
authors: Fu
mesh_terms: D001280:Atrazine / Q000009:adverse effects*; D048408:Chlorella vulgaris / Q000187:drug effects*; D056890:Eukaryota / Q000187:drug effects; D006540:Herbicides / Q000009:adverse effects; D010088:Oxidoreductases / Q000187:drug effects; D045332:Photosystem II Protein Complex / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects; D014873:Water Pollutants / Q000009:adverse effects*
chemical_list: D006540:Herbicides; D045332:Photosystem II Protein Complex; D012333:RNA, Messenger; D014873:Water Pollutants; D010088:Oxidoreductases; D001280:Atrazine