title: Two biosensors for phenolic compounds based on mushroom (Agaricus bisporus) homogenate: comparison in terms of some important parameters of the biosensors.
abstract: Interest in molecular imprinted polymer techniques has increased because they allows for the improvement of some stability characteristics of enzymes. The high stability of molecularly imprinted enzymes for a substrate can make them ideal alternatives as recognition elements for sensors. A bioimprinted mushroom tissue homogenate biosensor was constructed in a very simple way. For this purpose, sulfite was used. The enzyme, polyphenol oxidase, was first complexed by using a competitive inhibitor, sulfite, in aqueous medium and then the enzyme was immobilized on gelatin by crosslinking with glutaraldehyde on a glass electrode surface. Similarly, polyphenol oxidase uncomplexed with sulfite was also immobilized on a glass electrode in the same conditions. The aim of the study was to compare the two biosensors in terms of their repeatability and thermal, pH, and operational stability; also, the linear ranges of the two biosensors were compared with each other.
authors: Faculty of Science and Arts, Chemistry Department, Muğla University, Muğla, Türkiye.
authors: Mustafa
authors: M
authors: Teke
authors: M Kemal
authors: MK
authors: Sezgintürk
authors: Erhan
authors: E
authors: Dinçkaya
authors: Azmi
authors: A
authors: Telefoncu
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004156:Catechol Oxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D002396:Catechols / Q000032:analysis; D003432:Cross-Linking Reagents / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004800:Enzymes, Immobilized; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D005780:Gelatin / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013447:Sulfites / Q000494:pharmacology; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D002396:Catechols; D003432:Cross-Linking Reagents; D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized; D010636:Phenols; D013447:Sulfites; D014867:Water; D005780:Gelatin; D004156:Catechol Oxidase; D005976:Glutaral