title: Purification and characterization of transcribed RNAs using gel filtration chromatography.
abstract: RNA synthesis using in vitro transcription by phage T7 RNA polymerase allows preparation of milligram quantities of RNA for biochemical, biophysical and structural investigations. Previous purification approaches relied on gel electrophoretic or gravity-flow chromatography methods. We present here a protocol for the in vitro transcription of RNAs and subsequent purification using fast-performance liquid chromatography. This protocol greatly facilitates production of RNA in a single day from transcription to purification.
authors: Department of Structural Biology, Stanford University School of Medicine, Stanford, California, USA.
authors: Sean A
authors: SA
authors: McKenna
authors: Insil
authors: I
authors: Kim
authors: Elisabetta Viani
authors: EV
authors: Puglisi
authors: Darrin A
authors: DA
authors: Lindhout
authors: Colin Echeverría
authors: CE
authors: Aitken
authors: R Andrew
authors: RA
authors: Marshall
authors: Joseph D
authors: JD
authors: Puglisi
mesh_terms: D002850:Chromatography, Gel; D010957:Plasmids; D012313:RNA / Q000032:analysis*; D016337:RNA, Catalytic; D045162:Thiazolidinediones; D014158:Transcription, Genetic
chemical_list: D016337:RNA, Catalytic; D045162:Thiazolidinediones; C427204:hammerhead ribozyme; C114240:DN 108; D012313:RNA