Liver tissue responses to gelatin and gelatin/chitosan gels.
Gelatin and gelatin/chitosan gels, crosslinked using glutaraldehyde, were previously developed as substrates for three-dimensional cell-assembly techniques. In this study, the biocompatibility and biodegradation of gelatin and gelatin/chitosan gels were evaluated following implantation in rat livers for periods up to 16 weeks. The two gels were characterized by different inflammatory responses and degradation rates. The gelatin/chitosan gel is more efficient in inducing fibrin formation and vascularization at the implant-host interface. The degrees of inflammatory reaction for the gelatin/chitosan gel were significantly stronger than the gelatin gel. Advanced biodegradation of the gelatin gels was observed. These data indicate that the gelatin gel has better liver tissue biocompatibility and a faster biodegraded rate than the gelatin/chitosan gel.
Wang
Xiaohong
X
Key Laboratory for Advanced Manufacturing by Materials Processing Technology and Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Yu
Xin
X
Yan
Yongnian
Y
Zhang
Renji
R
D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001673:Biodegradation, Environmental; D048271:Chitosan / Q000494:pharmacology*; D005260:Female; D005337:Fibrin / Q000276:immunology; D005549:Foreign-Body Reaction / Q000139:chemically induced; D005780:Gelatin / Q000494:pharmacology*; D005782:Gels / Q000494:pharmacology; D006098:Granulocytes / Q000276:immunology; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D008422:Materials Testing; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D005782:Gels; D005780:Gelatin; D005337:Fibrin; D048271:Chitosan