Primary and secondary infections of Macaca fascicularis monkeys with Asian and American genotypes of dengue virus 2.
The goal of this study was to compare the immune response and the protection capacity induced by the dengue virus 2 (DENV-2) American and Asian genotypes in Macaca fascicularis monkeys. Animals were infected with American or Asian DENV-2 strains and challenged 1 year later with a DENV-2 Asian genotype strain. The viremia and monkey antibody levels were similar for the different strains after primary and secondary infection; however, the functionality of the antibody response was different. A limited viral replication was demonstrated after the secondary infection in all the monkeys. No virus was isolated in tissue culture, while reverse transcription-PCR showed a late positive reaction in four of five challenged monkeys. The immunoglobulin M response pattern and the detection of antibodies to specific proteins by Western blotting supported the protection data. Despite the demonstration of the protective effect after homologous challenge, a strong anamnestic antibody response was observed.
Bernardo
Lidice
L
Department of Virology, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía, km 6 1/2, P.O. Box 601, Marianao 13, Havana, Cuba.
Izquierdo
Alienys
A
Prado
Irina
I
Rosario
Delfina
D
Alvarez
Mayling
M
Santana
Emidalys
E
Castro
Jorge
J
Martínez
Rafael
R
Rodríguez
Rosmari
R
Morier
Luis
L
Guillén
Gerardo
G
Guzmán
María G
MG
D000330:Aedes; D000569:Americas; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001208:Asia; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003715:Dengue / Q000276:immunology* / Q000503:physiopathology / Q000821:virology; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D005838:Genotype; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D008252:Macaca fascicularis; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019595:Severe Dengue / Q000276:immunology* / Q000503:physiopathology / Q000821:virology; D014709:Vero Cells; D014779:Virus Replication
D000914:Antibodies, Viral; D007075:Immunoglobulin M