[The molecular differences between genotype 1 and genotype 4 of HEV in their neutralization region].
HEV is classified into H (human) group and Z (zoonosis) group according to its compatible host. H group contains genotype 1 and genotype 2 HEV isolates which infect human only; Z group contains genotype 3 and genotype 4 HEV isolates which infect both human and animals. After analysis of amino acid sequences between ORF2 aa368 and aa606, four group-conserved sites that were all located in the neutralization region of ORF2 were identified. They are aa483, aa492, aa497 and aa599. Mutation analysis and capture PCR were then performed on these sites with a group of monoclonal antibodies. Results showed that the difference of the aa497 between H and Z groups was responsible for the maintenance of their group-specific immunodominant epitopes, probably through confirmation-dependent epitope changes. Thus, aa497 and its related change on the surface structure of HEV may play important roles in host selection by H and Z groups of HEV.
Guo
Qing-Shun
QS
National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen 361005, China).
Ge
Sheng-Xiang
SX
Xiong
Jun-Hui
JH
Yan
Qiang
Q
Li
Shao-Wei
SW
Gu
Ying
Y
Xu
Ping-Dong
PD
Shi
Wei-Guo
WG
Zhang
Jun
J
Xia
Ning-Shao
NS
D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D005838:Genotype; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D016056:Immunodominant Epitopes; D008969:Molecular Sequence Data; D009154:Mutation; D009500:Neutralization Tests; D016366:Open Reading Frames
D000911:Antibodies, Monoclonal; D016056:Immunodominant Epitopes