Assignment of the absolute configuration of P-chiral 5' mRNA cap analogues containing phosphorothioate moiety.
Enzymatic cleavage of the P-chiral diastereoisomers of the 5' mRNA cap analogue bearing phosphorothioate moiety in alfa position of 5',5'-triphosphate bridge (m(7)Gppp(S)G D1 and D2) was performed by human Decapping Scavenger (DcpS) enzyme. Analysis of the degradation products allowed to estimate the absolute configuration at the asymmetric phosphorus atoms in examined compounds via correlation with the R(P) and S(P) diastereoisomers of guanosine 5'-O-(1-thiodiphosphate) (GDPalphaS).
Kowalska
Joanna
J
Department of Biophysics, Institute of Experimental Physics, Warsaw University, Zwirki i Wigury 93, Warsaw 02-089, Poland.
Lewdorowicz
Magdalena
M
Zuberek
Joanna
J
Bojarska
Elzbieta
E
Stepinski
Janusz
J
Stolarski
Ryszard
R
Darzynkiewicz
Edward
E
Jemielity
Jacek
J
D004722:Endoribonucleases / Q000737:chemistry*; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives / Q000032:analysis; D006801:Humans; D006868:Hydrolysis; D009690:Nucleic Acid Conformation; D012314:RNA Cap Analogs / Q000737:chemistry*; D012315:RNA Caps / Q000737:chemistry*; D013873:Thionucleotides / Q000032:analysis
D012314:RNA Cap Analogs; D012315:RNA Caps; D013873:Thionucleotides; C527041:m(7)GpppsG nucleotide; D006153:Guanosine Diphosphate; C020339:guanosine 5'-O-(1-thiodiphosphate); D004722:Endoribonucleases; C485332:DcpS protein, human