title: Escherichia coli cytosolic glycerophosphodiester phosphodiesterase (UgpQ) requires Mg2+, Co2+, or Mn2+ for its enzyme activity.
abstract: Escherichia coli cytosolic glycerophosphodiester phosphodiesterase, UgpQ, functions in the absence of other proteins encoded by the ugp operon and requires Mg2+, Mn2+, or Co2+, in contrast to Ca2+-dependent periplasmic glycerophosphodiester phosphodiesterase, GlpQ. UgpQ has broad substrate specificity toward various glycerophosphodiesters, producing sn-glycerol-3-phosphate and the corresponding alcohols. UgpQ accumulates under conditions of phosphate starvation, suggesting that it allows the utilization of glycerophosphodiesters as a source of phosphate. These results clarify how E. coli utilizes glycerophosphodiesters using two homologous enzymes, UgpQ and GlpQ.
authors: RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan. ohshima-tky@umin.ac.jp
authors: Noriyasu
authors: N
authors: Ohshima
authors: Saori
authors: S
authors: Yamashita
authors: Naoko
authors: N
authors: Takahashi
authors: Chizu
authors: C
authors: Kuroishi
authors: Yoshitsugu
authors: Y
authors: Shiro
authors: Koji
authors: K
authors: Takio
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003035:Cobalt / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D015151:Immunoblotting; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D008670:Metals / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000378:metabolism*; D011522:Protons; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008670:Metals; D010710:Phosphates; D011522:Protons; D011994:Recombinant Proteins; D003035:Cobalt; D008345:Manganese; D010727:Phosphoric Diester Hydrolases; C087921:glpQ protein, Bacteria; C038133:glycerophosphodiester phosphodiesterase; D008274:Magnesium