title: An analytical method for determining relative specificities for sequential reactions catalyzed by the same enzyme: application to the hydrolysis of triacylglycerols by lipases.
abstract: We propose a model for the sequential hydrolysis of ester bonds of triacylglycerols by lipases and use it as the basis for an analytical method for determining the relative specificity of the lipase for the various substrates with which it can react, when the substrates occur simultaneously in a single reaction system. We then apply the method to our own data and literature data involving the hydrolysis of triacylglycerols by lipases. Our model is able to fit well to most of the reaction profiles, enabling the estimation of relative specificities. We discuss the limitations and potential applications of our method.
authors: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx.P. 19046, Centro Politécnico, Curitiba 81531-990, Paraná, Brazil. davidmitchell@ufpr.br
authors: David Alexander
authors: DA
authors: Mitchell
authors: Jorge A
authors: JA
authors: Rodriguez
authors: Frédéric
authors: F
authors: Carrière
authors: Jacques
authors: J
authors: Baratti
authors: Nadia
authors: N
authors: Krieger
mesh_terms: D000818:Animals; D002384:Catalysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004285:Dogs; D005990:Glycerol / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D008954:Models, Biological; D013379:Substrate Specificity; D034421:Sus scrofa; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D014280:Triglycerides; D008049:Lipase; D005990:Glycerol