title: Cyclosporin A induces peritoneal fibrosis and angiogenesis during chronic peritoneal exposure to a glucose-based, lactate-buffered dialysis solution in the rat.
abstract: OBJECTIVE
  Cyclosporin A (CsA) stimulates the development of fibrosis. We investigated whether CsA contributes to peritoneal alterations induced by long-term exposure to dialysis solutions.
  
  
  METHODS
  Ten rats received peritoneal infusion of dialysis solution and oral CsA for 8 weeks. Eight received only the dialysis solution (controls). Peritoneal function was assessed at 8 weeks followed by sacrifice. The number of vessels was counted, fibrosis was assessed and hydroxyproline was determined. PCR was performed for vascular endothelial growth factor (VEGF), connective tissue growth factor (CTGF) and transforming growth factor-beta (TGF-beta).
  
  
  RESULTS
  Histology revealed more fibrosis, hydroxyproline and vessels (thick walled) in CsA-exposed animals. Peritoneal transport was not different. The mRNA content of TGF-beta, CTGF and VEGF was higher in CsA.
  
  
  CONCLUSIONS
  CsA combined with exposure to dialysis solutions was associated with increased peritoneal fibrosis and angiogenesis.
authors: Department of Nephrology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors: Roos
authors: R
authors: van Westrhenen
authors: Jan
authors: J
authors: Aten
authors: Najat
authors: N
authors: Hajji
authors: Onno J
authors: OJ
authors: de Boer
authors: Cindy
authors: C
authors: Kunne
authors: Dirk R
authors: DR
authors: de Waart
authors: Raymond T
authors: RT
authors: Krediet
mesh_terms: D000818:Animals; D016572:Cyclosporine / Q000009:adverse effects*; D005355:Fibrosis / Q000139:chemically induced*; D005947:Glucose / Q000627:therapeutic use; D015312:Hemodialysis Solutions / Q000009:adverse effects* / Q000737:chemistry; D019344:Lactic Acid / Q000627:therapeutic use; D008297:Male; D009389:Neovascularization, Pathologic / Q000139:chemically induced*; D010530:Peritoneal Dialysis / Q000009:adverse effects*; D010532:Peritoneal Diseases / Q000139:chemically induced* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015312:Hemodialysis Solutions; D019344:Lactic Acid; D016572:Cyclosporine; D005947:Glucose