Clustered gene expression changes flank targeted gene loci in knockout mice.
BACKGROUND
Gene expression profiling using microarrays is a powerful technology widely used to study regulatory networks. Profiling of mRNA levels in mutant organisms has the potential to identify genes regulated by the mutated protein.


RESULTS
Using tissues from multiple lines of knockout mice we have examined genome-wide changes in gene expression. We report that a significant proportion of changed genes were found near the targeted gene.


CONCLUSIONS
The apparent clustering of these genes was explained by the presence of flanking DNA from the parental ES cell. We provide recommendations for the analysis and reporting of microarray data from knockout mice.
Valor
Luis M
LM
Genes to Cognition Programme, The Wellcome Trust Sanger Institute, Cambridge, United Kingdom.
Grant
Seth G N
SG
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D020869:Gene Expression Profiling; D006624:Hippocampus / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic; D014960:X Chromosome
D017931:DNA Primers