Effects of genistein and herbimycin, tyrosine kinase inhibitors, on catecholamine release in bovine adrenal chromaffin cells.
1 The effects of genistein and herbimycin, tyrosine kinase inhibitors, on catecholamine (CA) release were examined in bovine adrenal chromaffin cells. 2 In intact cells, genistein (10-100 microm) and herbimycin (3-30 microm) inhibited CA release induced by acetylcholine (ACh; 100 microm) or the nicotinic receptor stimulant 1,1-dimethyl-4-phenyl-piperazinium (DMPP; 10 microm), but did not affect CA release induced by high K+ (40 mm). 3 Genistein and herbimycin inhibited (45)Ca2+ uptake induced by ACh (100 microm). 4 Neither genistein nor herbimycin affected [(3)H]nicotine binding with nicotinic receptors. 5 In beta-escin-permeabilized cells, neither genistein nor herbimycin affected CA release induced by Ca2+ (1 microm). 6 These results suggest that protein tyrosine kinase plays the facilitatory role in the regulation of CA release induced by nicotinic receptor stimulation in stimulus-secretion coupling of bovine adrenal chromaffin cells.
Matsumura
C
C
Department of Pharmacology, The Nippon Dental University School of Life Dentistry at Niigata, Hamaura-cho, Chuou-ku, Niigata 951-8580, Japan.
Kuwashima
H
H
Soma
S
S
Kimura
T
T
D000818:Animals; D016227:Benzoquinones / Q000494:pharmacology*; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D019833:Genistein / Q000494:pharmacology*; D047029:Lactams, Macrocyclic / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D017828:Rifabutin / Q000031:analogs & derivatives
D016227:Benzoquinones; D002395:Catecholamines; D047029:Lactams, Macrocyclic; D047428:Protein Kinase Inhibitors; D017828:Rifabutin; C020754:herbimycin; D019833:Genistein