Impact of PAMAM G2 and G6 dendrimers on bovine serum albumin (fatty acids free and loaded with different fatty acids).
Dendrimers are a new class of nanotechnological polymers suitable for drug targeting, microarray systems or detoxication. The present study is devoted to a detailed analysis of binding between PAMAM dendrimers and bovine serum albumin (fatty acid free or loaded with oleic, linoleic, oleic+linoleic or oleic+linoleic+arachodonic acids) by measuring zeta-potential, fluorescence quenching, fluorescence anisotropy and electron paramagnetic resonance. Addition of PAMAM G2 and G6 dendrimers to protein solutions resulted in attachment to the protein molecule. The PAMAM dendrimers also competed with BSA for fatty acids if two or three fatty acids were loaded per protein. This can lead to the extraction of fatty acids from BSA to the PAMAM dendrimer.
Shcharbin
Dzmitry
D
Department of General Biophysics, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland. shcharbi@biol.uni.lodz.pl <shcharbi@biol.uni.lodz.pl>
Ottaviani
M Francesca
MF
Cangiotti
Michela
M
Przybyszewska
Magdalena
M
Zaborski
Marian
M
Bryszewska
Maria
M
D016880:Anisotropy; D050091:Dendrimers / Q000737:chemistry*; D004563:Electrochemistry; D004578:Electron Spin Resonance Spectroscopy; D005227:Fatty Acids / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D050091:Dendrimers; D005227:Fatty Acids; C104700:PAMAM Starburst; D011073:Polyamines; D012710:Serum Albumin, Bovine