Usefulness of a quick decalcification of bone sections embedded in methyl methacrylate[corrected]: an improved method for immunohistochemistry.
Immunohistochemistry of undecalcified bone sections embedded in methyl methacrylate (MMA) is not commonly employed because of potential destruction of tissue antigenicity by highly exothermic polymerization. The aim of the present study was to describe a new technique in which a quick decalcification of bone sections embedded in MMA improves the results for immunohistochemistry. The quality of interleukin 1alpha (IL-1alpha) immunostaining according to the present method was better than the conventional one. Immunostaining for osteoprotegerin (OPG) and the receptor activator of NF-kappaB ligand (RANKL) in bone sections of chronic kidney disease patients with mineral bone disorders (CKD-MBD) was stronger than in controls (postmortem healthy subjects). The present study suggested that this method is easy, fast, and effective to perform both histomorphometry and immunohistochemistry in the same bone fragment, yielding new insights into pathophysiological aspects and therapeutic approaches in bone disease.
Gomes
Samirah Abreu
SA
Division of Nephrology, Universidade Federal de São Paulo, Rua Botucatu, 740 Vila Clementino, São Paulo, SP Brazil 04023-062. samirah@nefro.epm.br
dos Reis
Luciene Machado
LM
de Oliveira
Ivone Braga
IB
Noronha
Irene de Lourdes
IL
Jorgetti
Vanda
V
Heilberg
Ita Pfeferman
IP
D001847:Bone Diseases / Q000473:pathology; D001842:Bone and Bones / Q000166:cytology*; D003648:Decalcification Technique / Q000379:methods*; D006801:Humans; D007085:Ilium / Q000473:pathology; D007150:Immunohistochemistry / Q000379:methods*; D007676:Kidney Failure, Chronic / Q000473:pathology; D020366:Methylmethacrylate; D016610:Tissue Embedding / Q000379:methods
D020366:Methylmethacrylate