title: CD30 on stimulated CD4+ T lymphocytes in newborns regarding atopic heredity.
abstract: CD30 was initially described as Ki-1 Ag on Reed-Sternberg cells of Hodgkin's lymphoma and its and CD30L(+) expression on T cells in placenta were equally frequent in the atopic and non-atopic women. In this article we present a study of CD30 mean fluorescence intensity (MFI) on CB T CD4(+) cells. We tested the hypothesis that in newborns with atopy family history there is a changed CB T cells response after antigen stimulation comparing with those without atopy family history. The study population consisted of 31 newborn babies (29-breastfed, two non-breastfed) and their mothers. Eleven of them had positive and 20 had negative atopy family history. Performed tests included cord blood, which was a subject to flowcytometry analysis and was cultured for 24 h, cytokine production was measured (IFN- gamma, IL-4 and IL-12). Secondly, we measured total maternal and cord blood IgE levels. We studied CD30 MFI as in our studies in larger group of newborns, CD30 expression on CD4(+) T cells appeared to be very low. MFI of CD4(+) CD30(+) after PHA-stimulation (213.55: range: 41.77-434.51) was significantly increased compared to MFI of CD4(+) CD30(+) before PHA-stimulation (43.63: range 28.67-134.67)(p </= 0.001). Newborns with and without atopy family history were analyzed. We found no difference between MFI of CD4(+) CD30(+) on non-stimulated T cells in non-atopic (43.80: range 28.66-134.66) and atopic (43.30: range 29.12-80.92) (p > 0.05). After PHA stimulation MFI of CD4(+) CD30(+) in non-atopic (273.05 (range: 42.9-434.51) was significantly increased compared with the atopic newborns to MFI of 87.1 (range: 41.78-241.42) (p = 0.00). We have not found any correlation between MFI of CD4(+) CD30(+) and total maternal and total CB IgE levels. The role of CD30 in immunological response needs further research studies.
authors: Department of Pediatrics, Medical University of Silesia, Bytom, Poland.
authors: Krystyna
authors: K
authors: Stencel-Gabriel
authors: Iwona
authors: I
authors: Gabriel
authors: Zbigniew
authors: Z
authors: Czuba
authors: Bogdan
authors: B
authors: Mazur
authors: Marek
authors: M
authors: Paul
authors: Paweł
authors: P
authors: Górski
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007231:Infant, Newborn; D007371:Interferon-gamma / Q000032:analysis* / Q000276:immunology; D018664:Interleukin-12 / Q000032:analysis / Q000276:immunology; D015847:Interleukin-4 / Q000032:analysis / Q000276:immunology; D017730:Ki-1 Antigen / Q000032:analysis* / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation
chemical_list: D017730:Ki-1 Antigen; D018664:Interleukin-12; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma