A real-time quantitative PCR detection method for pork, chicken, beef, mutton, and horseflesh in foods.
A rapid real-time quantitative PCR method to detect trace amounts of pork, chicken, beef, mutton, and horseflesh in foods was developed. The primers and TaqMan MGB (minor groove binder) probes were designed on the gene encoding cytochrome b for the specific detection of each species. The limit of quantification of this method was found to be 100 fg/microl of each mitochondrial DNA in 10 ng/microl of the wheat mitochondrial DNA matrix. The calculated R(2) values of the standard curves for the five species ranged between 0.994 and 0.999. This method would be particularly useful in the detection of hidden meat mince in processed foods, which would verify food labeling and gain consumers' trust.
Tanabe
Soichi
S
Graduate School of Biosphere Science, Hiroshima University, Hiroshima, Japan. stanabe@hiroshima-u.ac.jp
Hase
Makiko
M
Yano
Takeo
T
Sato
Masahiko
M
Fujimura
Tatsuya
T
Akiyama
Hiroshi
H
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002645:Chickens; D003257:Consumer Product Safety; D045303:Cytochromes b / Q000032:analysis*; D004272:DNA, Mitochondrial / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination; D006736:Horses; D008460:Meat / Q000032:analysis*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012756:Sheep; D013045:Species Specificity; D013552:Swine
D004272:DNA, Mitochondrial; D045303:Cytochromes b