title: Increased yield of porcine circovirus-2 by a combined treatment of PK-15 cells with interferon-gamma and inhibitors of endosomal-lysosomal system acidification.
abstract: Treatment of porcine kidney (PK-15) cells with either interferon-gamma (IFN-gamma) or endosomal- lysosomal system acidification inhibitors increases replication of porcine circovirus type 2 (PCV2). In the present study, the effect of a combination of these treatments on the number of infected cells and virus yield was tested. The number of PCV2 (Stoon-1010)-infected PK-15 cells increased in cells treated with ammonium chloride (445 +/- 39% increase), IFN-gamma (446 +/- 8%), ammonium chloride + IFN-gamma (1721 +/- 283%), chloroquine diphosphate (1037 +/- 121%), chloroquine diphosphate + IFN-gamma (2199 +/- 255%), monensin (950 +/- 178%) and monensin + IFN-gamma (1948 +/- 60%). Combined IFN-gamma and endosomal-lysosomal system acidification inhibitors increased PCV2 yield by up to 50 times compared to untreated PK-15. This augmented virus replication in PK-15 cells may be helpful in the production of PCV2 vaccines.
authors: Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
authors: G
authors: G
authors: Misinzo
authors: P L
authors: PL
authors: Delputte
authors: D J
authors: DJ
authors: Lefebvre
authors: H J
authors: HJ
authors: Nauwynck
mesh_terms: D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D002460:Cell Line / Q000187:drug effects; D002738:Chloroquine / Q000031:analogs & derivatives / Q000494:pharmacology; D017925:Circovirus / Q000254:growth & development*; D011992:Endosomes / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007155:Immunologic Factors / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D008985:Monensin / Q000494:pharmacology; D013552:Swine
chemical_list: D004791:Enzyme Inhibitors; D007155:Immunologic Factors; D000643:Ammonium Chloride; C023676:chloroquine diphosphate; D007371:Interferon-gamma; D002738:Chloroquine; D008985:Monensin