Combinatorial synthesis of peptide arrays onto a microchip.
Arrays promise to advance biology through parallel screening for binding partners. We show the combinatorial in situ synthesis of 40,000 peptide spots per square centimeter on a microchip. Our variant Merrifield synthesis immobilizes activated amino acids as monomers within particles, which are successively attracted by electric fields generated on each pixel electrode of the chip. With all different amino acids addressed, particles are melted at once to initiate coupling. Repetitive coupling cycles should allow for the translation of whole proteomes into arrays of overlapping peptides that could be used for proteome research and antibody profiling.
Beyer
Mario
M
German Cancer Research Center, INF 580, 69120 Heidelberg, Germany.
Nesterov
Alexander
A
Block
Ines
I
König
Kai
K
Felgenhauer
Thomas
T
Fernandez
Simon
S
Leibe
Klaus
K
Torralba
Gloria
G
Hausmann
Michael
M
Trunk
Ulrich
U
Lindenstruth
Volker
V
Bischoff
F Ralf
FR
Stadler
Volker
V
Breitling
Frank
F
D000596:Amino Acids / Q000737:chemistry; D020650:Combinatorial Chemistry Techniques; D004560:Electricity; D010455:Peptides / Q000138:chemical synthesis*; D040081:Protein Array Analysis / Q000379:methods
D000596:Amino Acids; D010455:Peptides