title: [Laboratory diagnosis and molecular characterization of highly pathogenic avian influenza virus (H5N1) in human in Hunan Province in 2005-2006].
abstract: To determine the etiologic agents of two atypical pneumonia human cases in Hunan Province in 2005-2006 and to study their pathogenic potential, the patients' respiratory tract samples and sera were collected. The respiratory tract samples were tested by real-time RT-PCR and RT-PCR methods, and the sera by hemagglutination-inhibition assay. Virus was isolated from case 2 and its genome was sequenced and analyzed. Results showed the H5 nucleic acid tests of two cases were positive. The H5-specific antibody titer of the convalescence serum of case 1 showed a 4-fold greater rise than that of the acute phase. And case 2's antibody titer of acute phase was negative. The two atypical pneumonia cases were confirmed as the avian influenza A (H5N1) infection cases. Viral strain A/Hunan/1/2006 was isolated from case 2. Phylogenetic and molecular analysis suggested that 8 gene segments of A/Hunan/1/2006 originated from avian viruses. And A/Hunan/1/2006 was similar with viruses isolated from avian in Hunan Province. The isolated virus did not recombine with human influenza viruses and no obvious variation was observed.
authors: Hunan Provincial Center for Disease Control and Prevention, Changsha 410005, China.
authors: Yi-Wei
authors: YW
authors: Huang
authors: Zi
authors: Z
authors: Li
authors: Hong
authors: H
authors: Zhang
authors: Yun-Zhi
authors: YZ
authors: Liu
authors: Le-Ying
authors: LY
authors: Wen
authors: Jun-Hua
authors: JH
authors: Li
authors: Li-Dong
authors: LD
authors: Gao
authors: Xin-Sheng
authors: XS
authors: Zhao
authors: Zhong-Jie
authors: ZJ
authors: Li
authors: Bi-Yun
authors: BY
authors: Chen
authors: Yu
authors: Y
authors: Lan
authors: Lei
authors: L
authors: Zhou
authors: Yue-Long
authors: YL
authors: Shu
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D002648:Child; D002681:China; D005260:Female; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis / Q000821:virology*; D008297:Male; D010802:Phylogeny
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus