title: Correlation between immune and neuronal parameters and stress perception in allergic asthmatics.
abstract: BACKGROUND
  Asthma is a chronic disease defined by airway inflammation, increased airway hyperresponsiveness and episodes of airway obstruction. Although there are abundant clinical and experimental data showing that stress may worsen asthma, the mechanisms linking stress to asthma are not well understood. By inducing a pro-inflammatory cytokine milieu, stress might enhance airway inflammation in bronchial asthma. We therefore investigated the correlation of stress perception and the cytokine profile of circulating lymphocytes in humans.
  
  
  METHODS
  Allergic asthmatic patients and healthy controls were evaluated for perceived level of stress, demographic and lung function data. Whole blood cells were obtained and stimulated by mitogen to assess intracellular IL-4, IFN-gamma and TNF-alpha by flow cytometry. Neurotrophins nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) were measured in serum.
  
  
  RESULTS
  Asthmatic patients showed significantly higher percentages of TNF-alpha-producing T cells than healthy controls. Only in asthmatic patients was stress perception correlated with percentages of TNF-alpha-producing T cells and serum BDNF levels, while forced expiratory volume in 1 s (% predicted) was negatively correlated to BDNF.
  
  
  CONCLUSIONS
  The results of our study support the hypothesis that stress deteriorates bronchial asthma by inducing a pro-inflammatory cytokine profile in allergic asthmatics. Stress management might provide a supplement therapy of allergic asthma.
authors: Department of Internal Medicine and Psychosomatics, Charité Center for Internal Medicine and Dermatology, Charité-Universitaetsmedizin, Berlin, Germany. ricarda.joachim@charite.de
authors: R A
authors: RA
authors: Joachim
authors: O
authors: O
authors: Noga
authors: V
authors: V
authors: Sagach
authors: G
authors: G
authors: Hanf
authors: H
authors: H
authors: Fliege
authors: R D
authors: RD
authors: Kocalevent
authors: E M
authors: EM
authors: Peters
authors: B F
authors: BF
authors: Klapp
mesh_terms: D000328:Adult; D000368:Aged; D001249:Asthma / Q000175:diagnosis / Q000276:immunology*; D019208:Brain-Derived Neurotrophic Factor / Q000097:blood; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020932:Nerve Growth Factor / Q000097:blood; D009414:Nerve Growth Factors / Q000097:blood*; D012130:Respiratory Hypersensitivity / Q000175:diagnosis / Q000276:immunology*; D013312:Stress, Physiological / Q000150:complications* / Q000175:diagnosis; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D016207:Cytokines; D009414:Nerve Growth Factors; D020932:Nerve Growth Factor