Roles of the genetic variation of alcohol-metabolizing enzymes on biomarkers in trauma patients with excessive alcohol intake at emergency department.
BACKGROUND
Alcohol abuse has been implicated as an important factor for accidents. We evaluated the roles of different genetic combinations of the ADH2 and ALDH2 genotypes on biomarkers in trauma patients with excessive alcohol intake at our emergency department.


METHODS
Blood samples were obtained from 80 patients and 88 age-matched controls. The biomarkers, including AST, ALT, GGT, and MDA, were assayed. The polymerase chain reaction-restriction fragment length polymorphism method was used to determine the genetic polymorphisms of ADH2 and ALDH2.


RESULTS
There were significant differences in the levels of AST, ALT, GGT, MDA, and AST/ALT ratios between the 2 groups. In addition, MDA values and AST/ALT ratios were significantly higher in the patients with normal activity of ADH2 than the patients with low activity of ADH2. Meanwhile, regarding ALDH2 genotypes, there were significantly higher ratios of AST/ALT in the patients with low activity of ALDH2. The highest AST/ALT ratios and MDA values were in the patients with ADH2 (*2/*2) and ALDH2 (*1/*2 and *2/*2).


CONCLUSIONS
In conclusion, our results indicated that alcohol-induced liver damage or oxidative stress might be influenced by the genetic variation of ADH2 or ALDH2. Therefore, the combinations of different ADH2 and ALDH2 genotypes may be influential markers for susceptibility to alcohol-induced liver damage.
Tseng
Yang-Ming
YM
Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Jin
Yi-Ru
YR
Chen
Ing-Jun
IJ
Huang
Fong-Dee
FD
Wu
Szu-Hsien
SH
Ma
Hsu
H
Chen
Sheng-Yi
SY
Tsai
Li-Yu
LY
Tsai
Shih-Meng
SM
Lee
Jang-Hwa
JH
D000328:Adult; D000368:Aged; D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000435:Alcoholic Intoxication / Q000201:enzymology*; D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D004636:Emergency Service, Hospital; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008875:Middle Aged; D014947:Wounds and Injuries / Q000201:enzymology*
D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase