A flexible method for the fabrication of gold nanostructures using oligonucleotide derivatives.
Several linear and branched DNA structures from 80-200 nm with a biotine molecule in the middle have been prepared. These structures have been decorated by addition of positively charged gold nanoparticles carrying 4-(dimethylamino)pyridine ligands. Streptavidin binds to the central biotine molecule introducing a 20 nm gap in the structure in which a biotinylated nanoparticle can be introduced. The simplest structure (80 nm, linear) is formed by 4 oligonucleotides. By changing some of these components changes on length, shape, and recognition system easily can be introduced.
Eritja
Ramon
R
Instituto de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Cientificas, Jordi Girona 18-26, 08034 Barcelona, Spain. recgma@cid.csic.es
Aviñó
Anna
A
de la Torre
Beatriz G
BG
Fitzmaurice
Donald
D
Ongaro
Andrea
A
Stanca
Sarmiza Elena
SE
DiSalvo
Andrea
A
Maninng
Brendan
B
Iacopino
Daniela
D
D001710:Biotin / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D008024:Ligands; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D036103:Nanotechnology / Q000379:methods*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry
C417980:4-(dimethylamine)pyridine; D008024:Ligands; D009838:Oligodeoxyribonucleotides; D011725:Pyridines; D001710:Biotin; D006046:Gold; D004247:DNA; D019809:Streptavidin