title: Gene alteration and precursor and mature microRNA transcription changes contribute to the miRNA signature of primary effusion lymphoma.
abstract: MicroRNAs are regulated by gene alteration, transcription, and processing. Thus far, few studies have simultaneously assessed all 3 levels of regulation. Using real-time quantitative polymerase chain reaction (QPCR)-based arrays, we determined changes in gene copy number, pre-miRNA, and mature miRNA levels for the largest set of primary effusion lymphomas (PELs) to date. We detected PEL-specific miRNA gene amplifications, and concordant changes in pre-miRNA and mature miRNA. We identified 68 PEL-specific miRNAs. This defines the miRNA signature of PEL and shows that transcriptional regulation of pre-miRNA as well as mature miRNA levels contribute nonredundant information that can be used for the classification of human tumors.
authors: Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, Center for AIDS Research, and Curriculum in Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors: Andrea J
authors: AJ
authors: O'Hara
authors: Wolfgang
authors: W
authors: Vahrson
authors: Dirk P
authors: DP
authors: Dittmer
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D007150:Immunohistochemistry; D008223:Lymphoma / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D035683:MicroRNAs; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm