[Feasibilities of photodynamic therapy in the treatment of corneal mycotic lesions: an experimental study].
The paper presents the results of studying the photofungicidal activity of a photosens (octacationic phthalocyanine) in the in vitro and in vivo systems. Cultured Candida guilliermondii were employed in in vitro investigations. The single combined action of octacationic phthalocyanine at a concentration of up to 1 microM and laser irradiation at a wavelength of 675 nm were established to allow the high activation (up to 100%) of yeast fungi to be achieved in the in vitro system. In vivo investigations were performed on 14 rabbits (28 eyes) with induced keratomycosis. In the first series, fluorescent diagnosis of photosens accumulation in the pathological corneal areas was made in 9 (18 eyes). Based on the data obtained in the second series of experiments, the authors examined the feasibilities of photodynamic therapy for corneal mycotic lesions with the above photosensitizer in 5 rabbits (10 eyes).
Mamikonian
V R
VR
Balaian
M L
ML
Budzinskaia
M V
MV
Strakhovskaia
M V
MV
Fedorov
A A
AA
Loshchenov
V B
VB
Shevchik
S A
SA
Negrimovskiĭ
V M
VM
Iuzhakova
O A
OA
Kuz'min
S G
SG
Vorozhtsov
G N
GN
D000818:Animals; D002175:Candida / Q000302:isolation & purification; D002177:Candidiasis / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D015169:Colony Count, Microbial; D003315:Cornea / Q000187:drug effects / Q000382:microbiology* / Q000648:ultrastructure; D004195:Disease Models, Animal; D015821:Eye Infections, Fungal / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D005240:Feasibility Studies; D005500:Follow-Up Studies; D007211:Indoles / Q000627:therapeutic use*; D007634:Keratitis / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D008297:Male; D008854:Microscopy, Electron; D009942:Organometallic Compounds / Q000627:therapeutic use*; D010778:Photochemotherapy / Q000379:methods*; D011817:Rabbits; D011838:Radiation-Sensitizing Agents / Q000627:therapeutic use*; D016896:Treatment Outcome
D007211:Indoles; D009942:Organometallic Compounds; D011838:Radiation-Sensitizing Agents; C048577:aluminum tetrasulfophthalocyanine