Isolation and quantification of infectious pancreatic necrosis virus from ovarian and seminal fluids of Atlantic salmon, Salmo salar L.
Methods for the isolation and quantification of infectious pancreatic necrosis virus (IPNV) from ovarian and seminal fluids of Atlantic salmon are described. Both have utility for the non-lethal detection of IPNV in mature broodstock and for research into vertical transmission. Two experiments are described to check the efficiency of an elution method for the removal of IPNV from milt. The isolation rate for ovarian fluid of females was generally higher than that for seminal fluid of males from the same populations. In IPNV milt mixing experiments up to 99.98% of available IPNV adsorbed to Atlantic salmon spermatozoa and 20-100% of virus eluted using a variety of procedures. Titration of virus from naturally infected milt can be useful in estimating the relative vertical transmission risk from male broodstock.
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Fisheries Research Services, Marine Laboratory, Aberdeen, UK. smailda@marlab.ac.uk
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D000818:Animals; D018175:Birnaviridae Infections / Q000662:veterinary* / Q000821:virology; D005260:Female; D005393:Fish Diseases / Q000821:virology*; D018061:Infectious pancreatic necrosis virus / Q000254:growth & development / Q000302:isolation & purification*; D007668:Kidney / Q000821:virology; D008297:Male; D010053:Ovary / Q000821:virology; D015203:Reproducibility of Results; D020317:Salmo salar / Q000821:virology*; D012606:Scotland; D012661:Semen / Q000821:virology; D014773:Virology / Q000379:methods*