title: Kinetics of infective juvenile production of the entomopathogenic nematode Steinernema carpocapsae in submerged monoxenic culture.
abstract: The effects of culture medium formulations on the kinetics of infective juvenile (IJ) production of the entomopathogenic nematode Steinernema carpocapsae in submerged monoxenic culture, were studied at the cylindrical-bottle scale using six culture media containing agave juice from Agave spp. among other ingredients. The IJ production kinetics was well modelled through a re-parameterised 3-parameter Gompertz model with kinetic parameters: IJ-lag phase lambda (IJ) (day), maximum IJ-stage production rate m (max )(day(-1)), and IJ-multiplication factor (C (IJ)/C (IJ,0))(max)(-). The variation of lambda (IJ) was not very important within fermentations (10.3-16.2 days); nonetheless, important effects were observed on m (max) (32.8-241.2 days(-1)) and (C (IJ)/C (IJ,0))(max) (66(-) to 611.4(-)). Particularly, maximum values of m (max) and (C (IJ)/C (IJ,0))(max) were obtained in medium A4 (0.276 l l(-1) agave juice, 17 g l(-1) yeast extract, 12 g l(-1) dried egg yolk, 0.025 l l(-1) corn oil). Also, the maximum IJ concentration (249,444 per ml) was achieved in A4-fermentations.
authors: Centro de Investigaciones en Ciencia y Tecnología de los Alimentos, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Av. Universidad km 1, Rancho Universitario, Tulancingo de Bravo, Hidalgo, México. norberto@uaeh.reduaeh.mx
authors: Norberto
authors: N
authors: Chavarría-Hernández
authors: Marco-Antonio
authors: MA
authors: Islas-López
authors: Gabriela
authors: G
authors: Maciel-Vergara
authors: Martha
authors: M
authors: Gayosso-Canales
authors: Adriana-Inés
authors: AI
authors: Rodríguez-Hernández
mesh_terms: D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D003198:Computer Simulation; D007700:Kinetics; D008954:Models, Biological; D009348:Nematoda / Q000254:growth & development* / Q000382:microbiology*; D010572:Pest Control, Biological / Q000379:methods*; D020593:Xenorhabdus / Q000502:physiology*