title: Immunohistochemical localization of interleukin-6 in human pancreatitis.
abstract: The aim of the study was to identify immunohistochemically the localization of interleukin (IL)-6 in normal pancreas and in chronic pancreatitis (CP). Samples of tissues of normal pancreas (n=5) and CP (n=16), were verified histopathologically and then IL-6 was localized by immunohistochemical staining using the monoclonal antihuman IL-6 antibody and test LSAB2-HRP to visualize IL-6/Ab complexes. In slices of the pancreas, derived from patients with CP, a much stronger immunohistochemical reaction was noticed as compared with controls specimens. IL-6 was localized in exocrine, islet cells and ducts cells of the pancreas. Interestingly, this cytokine was detected in cytoplasm and very close to nucleus. Moreover, in cases of CP with inflammatory infiltration, there were a markedly stronger IL-6 expression, than that observed in specimens without infiltrate. In conclusion, the results presented herein clearly demonstrated a moderate and strong expression of IL-6 in exocrine and endocrine cells of patients with CP. These observations provide further support for the existence of local immune-pancreatic interactions.
authors: Department of Biomedical and Environmental Analyses, Wroclaw Meical University, Wroclaw, Poland. mch@tox.am.wroc.pl
authors: Monika
authors: M
authors: Jablonowska
authors: Halina
authors: H
authors: Milnerowicz
authors: Jerzy
authors: J
authors: Rabczynski
authors: Stanislaw
authors: S
authors: Milnerowicz
authors: Stanislaw
authors: S
authors: Nabzdyk
authors: Dariusz
authors: D
authors: Patrzalek
authors: Artur
authors: A
authors: Milnerowicz
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000032:analysis*; D007515:Islets of Langerhans / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D010179:Pancreas / Q000737:chemistry* / Q000473:pathology; D010183:Pancreatic Ducts / Q000737:chemistry; D050500:Pancreatitis, Chronic / Q000378:metabolism* / Q000473:pathology
chemical_list: D015850:Interleukin-6