[Primary investigation on metabolism of hpt gene in transgenic rice modified with cowpea trypsin inhibitor gene in rats].
OBJECTIVE
The metabolism characteristic in the gastrointestinal tract and possibility of transfer from gastrointestinal tract into liver and kidney was investigated.


METHODS
48 SD rats were allotted into 8 subgroups randomly, 6 groups rats of them were fed with transgenic rice diets and 2 groups rats were fed with non-transgenic rice diets. All rats were fed with transgenic and non-transgenic rice for two weeks. All rats were fast and sacrificed, the contents of stomach, jejunum, the lower part of ileum, cecum and rectum were taken out at 2, 4, 6, 8, 10 and 24 hours respectively after eating final diets. Fed two groups of SD rats with transgenic diet and non-transgenic rice diet respectively for 4 weeks, the liver and kidney were sampled and stored. All samples was examined by PCR.


RESULTS
The fragment of hpt was found the was only detected in stomach and jejunum, but none of the five fragments was detected in the lower part of ileum, cecum and rectum. The degradation of hpt was time-relationship and long-length fragment relationship, the longer was the action time, the more serious was the degradation. Foreign hpt gene was not detected in liver and kidney.


CONCLUSIONS
hpt gene was easy to degraded in gastrointestinal tract and was not detectable in hepatic tissue and nephridial tissues.
Xu
Haibin
H
Institute of Nutrition and Food Safety, Chinese for Disease Control and Prevention, Beijing 100021, China. hbxu1231602@vip.sina.com
Yin
Zhaoxue
Z
D000818:Animals; D008297:Male; D012275:Oryza / Q000009:adverse effects / Q000235:genetics*; D018532:Pisum sativum / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000009:adverse effects / Q000235:genetics* / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000009:adverse effects / Q000235:genetics*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014361:Trypsin Inhibitors / Q000235:genetics
D010940:Plant Proteins; D014361:Trypsin Inhibitors; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase