[Ectopic bone induction in vivo after transplantation of skeletal satellite cells from green fluorescence protein transgenic mouse transfected by adenoviral vectors encoding bone morphogenetic protein-2].
OBJECTIVE
To observe the ability of induced ectopic bone using skeletal muscles satellite cells (SMSCs) from newborn green fluorescence protein (GFP) transgenic mice mediated by Ad-BMP2.


METHODS
Transplantation of SMSCs transduced with Ad-BMP2 into back lamb muscles of subfascia in wildtype 129sv mice with a complex of collagen scaffords, then the tissue histologic examination, X ray plain film, fluorescence microscopy were used.


RESULTS
Transplantation of SMSCs transfected with Ad-BMP2 into back lamb muscles of subfascia generated ectopic bone formation involving GFP-positive osteoblasts and osteocytes 2 weeks and mature bone formation 4 weeks after transplantation. SMSCs non-transfected with Ad-BMP2 failed to induce ectopic bone formation.


CONCLUSIONS
SMSCs retain differentiation potentitality into osteoblasts in response to Ad-BMP2. They are useful tools for analyzing the process of osteoblast differentiation in vivo after transplantation.
Zhang
Feng-he
FH
Department of Oral and Maxillofacial Surgery, Stomatology School of Shandong University, Jinan 250012, China.
Huang
Ping
P
Wei
Feng-cai
FC
Sun
Shu-yang
SY
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D001842:Bone and Bones; D002454:Cell Differentiation; D005453:Fluorescence; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D051379:Mice; D008822:Mice, Transgenic; D032446:Myoblasts; D010006:Osteoblasts; D014162:Transfection; D016212:Transforming Growth Factor beta
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D016212:Transforming Growth Factor beta; D049452:Green Fluorescent Proteins