Activin receptor subunits in normal and dysfunctional adult human testis.
BACKGROUND
The cellular sites of activin action and its regulation in the normal and dysfunctional adult human testis are unknown.


METHODS
Activin type I (ALK2 and ALK4) and type II (ActRIIA and ActRIIB) receptors were detected using immunohistochemistry on Bouins fixed sections of normal, carcinoma in situ (CIS), seminoma, non-seminoma and gonadotropin-deprived human testis. ActRIIA mRNA was localized by in situ hybridization.


RESULTS
ALK2, ALK4 and ActRIIB proteins were observed in Sertoli cells, spermatogonia and some spermatocytes within normal and gonadotropin-suppressed adult human testis; all three receptor subunits were also detected in CIS, seminoma and non-seminoma cells. ActRIIA immunoreactivity was faint to absent in the normal testis and in CIS and non-seminoma cells, whereas some seminoma cells displayed a strong signal. Also in contrast to the normal testis, a majority of spermatogonia and Sertoli cells in gonadotropin-deprived samples exhibited a strong ActRIIA immunohistochemical and in situ hybridization signal.


CONCLUSIONS
Spermatogonia and Sertoli cells appear as the primary targets of activin action in the adult human testis. Changes in testicular function associated with altered hormone levels may enhance ActRIIA mRNA and protein synthesis, thus modifying signalling by activin or other TGFbeta ligands within specific cells of the seminiferous epithelium.
Dias
V
V
Monash Institute of Medical Research, 27-31 Wright Street Clayton, Victoria 3168, Australia.
Meachem
S
S
Rajpert-De Meyts
E
E
McLachlan
R
R
Manuelpillai
U
U
Loveland
K L
KL
D029404:Activin Receptors / Q000235:genetics / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D002278:Carcinoma in Situ / Q000378:metabolism*; D004357:Drug Synergism; D006062:Gonadotropins / Q000037:antagonists & inhibitors; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018239:Seminoma / Q000378:metabolism*; D012708:Sertoli Cells / Q000378:metabolism; D013090:Spermatocytes / Q000378:metabolism; D013093:Spermatogonia / Q000378:metabolism; D013736:Testicular Neoplasms / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology; D014018:Tissue Distribution
D006062:Gonadotropins; D020033:Protein Isoforms; D012333:RNA, Messenger; D013739:Testosterone; D017258:Medroxyprogesterone Acetate; D029404:Activin Receptors