title: [Study of anticoagulant activity of ethanol extracts from leech in vitro].
abstract: OBJECTIVE
  To study the anticoagulant activity of different portions of leech ethanol extracts (LEEs).
  
  
  METHODS
  Anticoagulant activity was determined by measuring prothrombin time(PT), thrombin time (TT), activated partial throboplstin time (APTT) and fibrinogen coagulation time (FCT).
  
  
  RESULTS
  PT, TT, APTT and FCT were remarkably prolonged by ethyl acetate portion of LEEs. Portions of petroleum ethrer, n-butanol and water extracted from LEEs was much weaker on anticoagulant activity.
  
  
  CONCLUSIONS
  The anticoagulant effect of ethyl acetate extract portion of LEEs is the strongest among the four portions, and this results may be from its inhibitory effect on thrombin-catalyzed fibrinogen hydrolysis.
authors: Institute of Pharmaceutical Science, College of Pharmaceutical Science, Southern Medical University, Guangzhou 510515, China.
authors: Guang-jun
authors: GJ
authors: Feng
authors: Zheng-guang
authors: ZG
authors: Zhu
authors: Chuan-lin
authors: CL
authors: Yu
authors: Qun
authors: Q
authors: Zhang
authors: Na-na
authors: NN
authors: Chen
authors: Lin-sheng
authors: LS
authors: Lei
authors: Shu-guang
authors: SG
authors: Wu
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000302:isolation & purification / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D001780:Blood Coagulation Tests; D000431:Ethanol; D006801:Humans; D007865:Leeches / Q000737:chemistry*; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D013918:Thrombin Time
chemical_list: D000925:Anticoagulants; D008419:Materia Medica; D000431:Ethanol