title: Her-2 protein expression, cellular localization, and gene amplification in colorectal carcinoma.
abstract: This study was sought to evaluate the relationship between Her-2 protein expression, cellular localization, gene amplification, and other clinicopathologic parameters in colorectal carcinomas. Her-2 protein expression and gene amplification were assessed in paraffin sections from 106 primary colorectal adenocarcinoma cases using immunohistochemistry and fluorescence in situ hybridization. Both membranous and cytoplasmic immunostaining was evaluated. The results were correlated with each other and with tumor grade, stage, and overall survival. Membranous and cytoplasmic protein expression was identified in 6 (5.6%) and 13 (12.26%) cases, respectively. Gene amplification was detected in 4 (3.7%) cases. There was a high concordance between membranous protein expression and gene amplification (kappa=0.791). No apparent association with any of the clinicopathologic parameters was identified. Membranous Her-2 protein expression and gene amplification are encountered in a small subset of colorectal carcinomas and are highly concordant events. Cytoplasmic protein expression might be either artifactual or it might represent a cross-reacting protein or a precursor form of the mature protein.
authors: Pathology Department, Propaedeutic, Athens University Medical School, Attikon University Hospital, Kyprou 9 st., 14562 Kifissia-Athens, Hellas. epavlaki@med.uoa.gr
authors: Kitty
authors: K
authors: Pavlakis
authors: Panteleimon
authors: P
authors: Kountourakis
authors: Efstathios
authors: E
authors: Stathopoulos
authors: Amanda
authors: A
authors: Psyrri
authors: Dimitra
authors: D
authors: Rontogianni
authors: Maria
authors: M
authors: Kafousi
authors: Maria
authors: M
authors: Derivianaki
authors: Nikolaos
authors: N
authors: Xiros
authors: Dimitrios
authors: D
authors: Pectasides
authors: Theofanis
authors: T
authors: Economopoulos
mesh_terms: D016477:Artifacts; D002277:Carcinoma / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D002462:Cell Membrane / Q000737:chemistry; D015179:Colorectal Neoplasms / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D003593:Cytoplasm / Q000737:chemistry; D005260:Female; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases