title: Heparinase selectively sheds heparan sulphate from the endothelial glycocalyx.
abstract: A healthy vascular endothelium is coated by the endothelial glycocalyx. Its main constituents are transmembrane syndecans and bound heparan sulphates. This structure maintains the physiological endothelial permeability barrier and prevents leukocyte and platelet adhesion, thereby mitigating inflammation and tissue oedema. Heparinase, a bacterial analogue to heparanase, is known to attack the glycocalyx. However, the exact extent and specificity of degradation is unresolved. We show by electron microscopy, immunohistological staining and quantitative measurements of the constituent parts, that heparinase selectively sheds heparan sulphate from the glycocalyx, but not the syndecans.
authors: Clinic of Anaesthesiology, Ludwig Maximilians University, Nussbaumstr. 20, D-80336 Munich.
authors: Daniel
authors: D
authors: Chappell
authors: Matthias
authors: M
authors: Jacob
authors: Markus
authors: M
authors: Rehm
authors: Mechthild
authors: M
authors: Stoeckelhuber
authors: Ulrich
authors: U
authors: Welsch
authors: Peter
authors: P
authors: Conzen
authors: Bernhard F
authors: BF
authors: Becker
mesh_terms: D000818:Animals; D003331:Coronary Vessels / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism* / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000648:ultrastructure; D019276:Glycocalyx / Q000378:metabolism*; D006168:Guinea Pigs; D019763:Heparin Lyase / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D010477:Perfusion; D053668:Syndecan-1 / Q000378:metabolism
chemical_list: D019763:Heparin Lyase; D006497:Heparan Sulfate; D053668:Syndecan-1