The nucleoporin Nup358 associates with and regulates interphase microtubules.
The nucleoporin Nup358 resides on the cytoplasmic face of the interphase nuclear pore complex (NPC). During metaphase, its recruitment to kinetochores is important for correct microtubule-kinetochore attachment. Here, we report that a fraction of endogenous Nup358 interacts with interphase microtubules through its N-terminal region (BPN). Cells overexpressing the microtubule targeting domain of Nup358 displayed dramatic alteration in the microtubule organization including increased microtubule bundling and stability. Ectopic expression of BPN and full-length Nup358 exhibited significantly higher levels of acetylated microtubules that were resistant to nocodazole, a microtubule depolymerizing agent. Furthermore, RNAi mediated depletion of Nup358 affected polarized stabilization of microtubules during directed cell migration, confirming the in vivo role of Nup358 in regulating interphase microtubules.
Joseph
Jomon
J
National Centre for Cell Science, Ganeshkhind, Pune 411 007, India. josephj@nccs.res.in
Dasso
Mary
M
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D019556:COS Cells; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D017931:DNA Primers; D007399:Interphase; D008870:Microtubules / Q000502:physiology*; D018832:Molecular Chaperones / Q000502:physiology*; D028861:Nuclear Pore Complex Proteins / Q000502:physiology*; D034622:RNA Interference
D017931:DNA Primers; D018832:Molecular Chaperones; D028861:Nuclear Pore Complex Proteins; C094493:ran-binding protein 2