title: Contribution of the conserved A16Leu to insulin foldability.
abstract: Contributions of the evolutionarily conserved A16Leu and B17Leu to insulin foldability were characterized by evaluating folding properties of single-chain insulin analogs. The results showed A16Leu had much more significant effects on the foldability of insulin than B17Leu.
authors: Institute of Molecular Biology, College of Life Sciences, Nan Kai University, Tian Jin 300071, China.
authors: Zhao-Jun
authors: ZJ
authors: Zhang
authors: Lan
authors: L
authors: Wu
authors: Zhi-Song
authors: ZS
authors: Qiao
authors: Ming-Qiang
authors: MQ
authors: Qiao
authors: You-Min
authors: YM
authors: Feng
authors: Zhan-Yun
authors: ZY
authors: Guo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D004220:Disulfides / Q000737:chemistry; D007328:Insulin / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007930:Leucine / Q000032:analysis*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D011384:Proinsulin / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011487:Protein Conformation; D017510:Protein Folding; D021381:Protein Transport; D013552:Swine
chemical_list: D004220:Disulfides; D007328:Insulin; D011384:Proinsulin; D007930:Leucine