title: Direct acoustic profiling of DNA hybridisation using HSV type 1 viral sequences.
abstract: We describe the detection of specific, conserved DNA sequences of herpes simplex virus (HSV) type 1 by means of a novel, high sensitivity acoustic biosensor. Repeated assays on planar and polymeric carboxylic acid- and biotin-presenting surface chemistries enabled statistical comparison of assay specificity and sensitivity and evaluation of assay Z-factor scores. Using a three minute hybridisation with NeutrAvidin capture for signal enhancement, it was possible to detect HSV viral nucleic acids at 5.2 x 10(-11) M concentration.
authors: Akubio Ltd, 181 Cambridge Science Park, Cambridge, UK CB4 0GJ.
authors: Yildiz
authors: Y
authors: Uludağ
authors: Xin
authors: X
authors: Li
authors: Heather
authors: H
authors: Coleman
authors: Stacey
authors: S
authors: Efstathiou
authors: Matthew A
authors: MA
authors: Cooper
mesh_terms: D000162:Acoustics; D015374:Biosensing Techniques; D004279:DNA, Viral / Q000032:analysis*; D018259:Herpesvirus 1, Human / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral