Sequence analysis of the regions flanking terminal repeats of the genome of umava isolate of murine gammaherpesvirus.
The umava isolate of murine gammaherpesvirus (MHV-umava) slightly differs from Murine gammaherpesvirus 68 (MHV-68) and two other isolates of murine gammaherpesvirus (MHV), MHV-76 and MHV-72 in some biological properties. To identify the region(s) in the MHV-umava genome responsible for this phenomenon, we compared the sequences flanking terminal repeats (TRs) of the MHV-umava genome with those of MHV-68, MHV-76 and MHV-72. Restriction and sequence analyses revealed in MHV-umava as compared to MHV-68 approximately 9.3 kbp deletion at the left end of the genome and approximately 1.5 kbp deletion at the right end of the genome. While the approximately 9.3 kbp deletion was similar to that in MHV-76, the approximately 1.5 kbp deletion was unique for MHV-umava.
Blaskovicova
J
J
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Tomaskova
J
J
Supolikova
M
M
Mistrikova
J
J
Kopacek
J
J
D024509:3' Flanking Region / Q000235:genetics*; D024506:5' Flanking Region / Q000235:genetics*; D000818:Animals; D016172:DNA Fingerprinting; D016679:Genome, Viral / Q000235:genetics*; D006566:Herpesviridae Infections / Q000821:virology; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D018144:Rhadinovirus / Q000235:genetics* / Q000302:isolation & purification; D012377:Rodentia / Q000821:virology; D017421:Sequence Analysis; D017384:Sequence Deletion; D020079:Terminal Repeat Sequences / Q000235:genetics*; D014412:Tumor Virus Infections / Q000821:virology