title: Transient transfection of Echinococcus multilocularis primary cells and complete in vitro regeneration of metacestode vesicles.
abstract: A major limitation in studying molecular interactions between parasitic helminths and their hosts is the lack of suitable in vitro cultivation systems for helminth cells and larvae. Here we present a method for long-term in vitro cultivation of larval cells of the tapeworm Echinococcus multilocularis, the causative agent of alveolar echinococcosis. Primary cells isolated from cultivated metacestode vesicles in vitro showed a morphology typical of Echinococcus germinal cells, displayed an Echinococcus-specific gene expression profile and a cestode-like DNA content of approximately 300Mbp. When kept under reducing conditions in the presence of Echinococcus vesicle fluid, the primary cells could be maintained in vitro for several months and proliferated. Most interestingly, upon co-cultivation with host hepatocytes in a trans-well system, mitotically active Echinococcus cells formed cell aggregates that subsequently developed central cavities, surrounded by germinal cells. After 4 weeks, the cell aggregates gave rise to young metacestode vesicles lacking an outer laminated layer. This layer was formed after 6 weeks of cultivation indicating the complete in vitro regeneration of metacestode larvae. As an initial step toward the creation of a fully transgenic strain, we carried out transient transfection of Echinococcus primary cells using plasmids and obtained heterologous expression of a reporter gene. Furthermore, we successfully carried out targeted infection of Echinococcus cells with the facultatively intracellular bacterium Listeria monocytogenes, a DNA delivery system for genetic manipulation of mammalian cells. Taken together, the methods presented herein constitute important new tools for molecular investigations on host-parasite interactions in alveolar echinococcosis and on the roles of totipotent germinal cells in parasite regeneration and metastasis formation. Moreover, they enable the development of fully transgenic techniques in this group of helminth parasites for the first time.
authors: University of Würzburg, Institute of Hygiene and Microbiology, Josef-Schneider-Strasse 2, D97080 Würzburg, Germany.
authors: Markus
authors: M
authors: Spiliotis
authors: Sabrina
authors: S
authors: Lechner
authors: Dennis
authors: D
authors: Tappe
authors: Carsten
authors: C
authors: Scheller
authors: Georg
authors: G
authors: Krohne
authors: Klaus
authors: K
authors: Brehm
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000502:physiology*; D004444:Echinococcosis, Hepatic / Q000469:parasitology*; D048210:Echinococcus multilocularis / Q000302:isolation & purification* / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology; D015801:Helminth Proteins / Q000302:isolation & purification* / Q000378:metabolism; D006790:Host-Parasite Interactions / Q000502:physiology; D007814:Larva / Q000254:growth & development; D008099:Liver / Q000469:parasitology; D014162:Transfection
chemical_list: D015801:Helminth Proteins