[Enhancing transfection efficiency of polyethylenimine by a hydrophobic peptide from bee venom].
The study was aimed to investigate the possibility of enhancing transfection efficiency of branched polyethylenimine (BPEI) in HeLa cells by hydrophobic tail of bee venom peptide (melittin). Hydrophobic tail of melittin was synthesized and its membrane permeable activity was evaluated by hemolysis test. The peptide was mixed with BPEI and the transfection efficiency was determined in HeLa cells by using green fluorescent protein gene (GFP) as a reporter gene. The cytotoxicity of the mixture was analyzed by MTT assay at 24 hours after transfection. The results indicated that the synthesized peptide had permeable activity leading to hemolysis in both neutral and acidic solution. At optimal condition, the peptide could significantly improve the transfection efficiency of BPEI and the cytotoxicity of the mixture was lower than BPEI itself. It is concluded that hydrophobic tail of melittin may be a potential enhancer to improve transfection efficiency mediated by cationic polymers in difficult to transfect cells.
Wang
Ying-Li
YL
Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Zhang
Yang-Pei
YP
Ji
Shou-Ping
SP
D006367:HeLa Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008555:Melitten / Q000737:chemistry* / Q000235:genetics; D010455:Peptides / Q000737:chemistry*; D011094:Polyethyleneimine / Q000494:pharmacology*; D014162:Transfection
D010455:Peptides; D008555:Melitten; D011094:Polyethyleneimine