The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8.
In this report, recombinant interleukin-8 (rIL-8) was produced and its activity tested for the first time in fish. The rainbow trout rIL-8 was produced in Escherichia coli and purified using a 6xHis tag at the N-terminus. The rIL-8 induced a dose-dependent migration of head kidney leukocytes at concentrations from 0.1 to 10 ng/ml, with a peak response at 1 ng/ml. Trout rIL-8 also had a significant effect on superoxide production by head kidney cells, with maximal activity at 0.1 and 1 ng/ml. When injected intraperitoneally into trout, rIL-8 had a clear effect on total leukocyte number in the peritoneal cavity, with increasing doses (up to 5 microg) eliciting more cells. Of three leukocyte types distinguished, neutrophils were the dominant cell type, especially at higher rIL-8 concentrations. In contrast, the proportion of macrophages and lymphocytes decreased with rIL-8 administration, suggesting that they were not attracted at the same rate as neutrophils.
Harun
Nor Omaima
NO
Scottish Fish Immunology Research Centre, University of Aberdeen, School of Biological Sciences, Aberdeen , UK.
Zou
Jun
J
Zhang
Yang-An
YA
Nie
Pin
P
Secombes
Chris J
CJ
D000818:Animals; D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli; D016209:Interleukin-8 / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D016897:Respiratory Burst / Q000187:drug effects
D016209:Interleukin-8; D011994:Recombinant Proteins