The karyotype and 5S rRNA genes from Spanish individuals of the bat species Rhinolophus hipposideros (Rhinolophidae; Chiroptera).
The karyotype of individuals of the species Rhinolophus hipposideros from Spain present a chromosome number of 2n = 54 (NFa = 62). The described karyotype for these specimens is very similar to another previously described in individual from Bulgaria. However, the presence of one additional pair of autosomal acrocentric chromosomes in the Bulgarian karyotype and the differences in X chromosome morphology indicated that we have described a new karyotype variant in this species. In addition, we have analyzed several clones of 1.4 and 1 kb of a PstI repeated DNA sequence from the genome of R. hipposideros. The repeated sequence included a region with high identity with the 5S rDNA genes and flanking regions, with no homology with GenBank sequences. Search for polymerase III regulatory elements demonstrated the presence of type I promoter elements (A-box, Intermediate Element and C-box) in the 5S rDNA region. In addition, upstream regulatory elements, as a D-box and Sp1 binding sequences, were present in flanking regions. All data indicated that the cloned repeated sequences are the functional rDNA genes from this species. Finally, FISH demonstrated the presence of rDNA in nine chromosome pairs, which is surprising as most mammals have only one carrier chromosome pair.
Puerma
Eva
E
Departamento de Biología Experimental, Facultad de Ciencias Experimentales y de la Salud, Universidad de Jaén, Paraje Las Lagunillas s/n, 23071, Jaen, Spain.
Acosta
Manuel J
MJ
Barragán
Maria José L
MJ
Martínez
Sergio
S
Marchal
Juan Alberto
JA
Bullejos
Mónica
M
Sánchez
Antonio
A
D000818:Animals; D001483:Base Sequence; D002685:Chiroptera / Q000235:genetics*; D003001:Cloning, Molecular; D005260:Female; D020459:Genes, rRNA; D017403:In Situ Hybridization; D007621:Karyotyping; D008297:Male; D008969:Molecular Sequence Data; D012341:RNA, Ribosomal, 5S / Q000032:analysis / Q000235:genetics*; D016415:Sequence Alignment; D013030:Spain
D012341:RNA, Ribosomal, 5S