title: Proliferation and neurogenesis of neural stem cells enhanced by cerebral microvascular endothelial cells.
abstract: In adult mammalian brain, vascular cells reside throughout life, close to central nervous system germinal zones, and neural stem cells (NSCs) mainly localize in the dentate gyrus of the hippocampus, subventricular zone, and olfactory bulb. Microvessels appear to produce a special microenvironment that may influence the characteristics of NSCs. To explore this potential correlation, an in vitro model with cocultured cerebral microvascular endothelial cells (CMECs) and NSCs was established in our study by using a transwell coculture system. The expression of nestin and NF in the early stage of coculture, and NF in the late stage, was detected by immunostaining. The results demonstrated that CMECs can stimulate self-renewal of NSCs and inhibit their differentiation, implying the potential of CMECs in promoting the neural differentiation of NSCs.
authors: Department of Neurosurgery, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China. gzguoying@hotmail.com
authors: Ying
authors: Y
authors: Guo
authors: Dejin
authors: D
authors: Shi
authors: Wensheng
authors: W
authors: Li
authors: Chaofeng
authors: C
authors: Liang
authors: Hui
authors: H
authors: Wang
authors: Zhuopeng
authors: Z
authors: Ye
authors: Liping
authors: L
authors: Hu
authors: Hua-Qiao
authors: HQ
authors: Wang
authors: Yan
authors: Y
authors: Li
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D054022:Cerebrum / Q000166:cytology*; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D016900:Neurofilament Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000502:physiology*
chemical_list: D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D016900:Neurofilament Proteins