title: Cloning and expression of a parathion hydrolase gene from a soil bacterium, Burkholderia sp. JBA3.
abstract: A bacterium, Burkholderia sp. JBA3, which can mineralize the pesticide parathion, was isolated from an agricultural soil. The strain JBA3 hydrolyzed parathion to p-nitrophenol, which was further utilized as the carbon and energy sources. The parathion hydrolase was encoded by a gene on a plasmid that strain JBA3 harbored, and it was cloned into pUC19 as a 3.7-kbp Sau3AI fragment. The ORF2 (ophB) in the cloned fragment encoded the parathion hydrolase composed of 526 amino acids, which was expressed in E. coli DH10B. The ophB gene showed no significant sequence similarity to most of other reported parathion hydrolase genes.
authors: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
authors: Taesung
authors: T
authors: Kim
authors: Jae-Hyung
authors: JH
authors: Ahn
authors: Min-Kyeong
authors: MK
authors: Choi
authors: Hang-Yeon
authors: HY
authors: Weon
authors: Mi Sun
authors: MS
authors: Kim
authors: Chi Nam
authors: CN
authors: Seong
authors: Hong-Gyu
authors: HG
authors: Song
authors: Jong-Ok
authors: JO
authors: Ka
mesh_terms: D019117:Burkholderia / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D006867:Hydrolases / Q000235:genetics*; D010278:Parathion / Q000378:metabolism*; D012988:Soil Microbiology
chemical_list: D010278:Parathion; D006867:Hydrolases