title: [Study on the histopathology of cats inoculated with H5N1 subtype high pathogenic avian influenza virus originated from tigers].
abstract: In this study, the HPAIV A/Tiger/Harbin/01/2002 (H5N1) used was originated from tigers and propagated in SPF embryonated hen eggs. TCID5, of the virus was 10(-7.36)/0. 05mL on MDCK cell. The cats were inoculated through bronchus route and then, the cats of dead and control were collected for histopathological and immunohistochemistry examination. Meanwhile, the emulsion supernatant fluid of organs and the pharyngeal swab samples of the dead cats were collected for RT-PCR, survived cats and the control cats were tested for the presence of HI antibody by standard method. The results indicated that the damage of lungs from the dead cats were most obvious, the wide range of red consolidation focus emerged on the lobus pulmonis, the fused focus of infection caused injury of lungs. Histology under the microscope revealed diffuse alveolar damage, confluence phlegmasia pathology, infiltration of lymphomonocytes, sackful of infiltration of macrophages and manipulus protein-like effusion in the alveolar. By immunohistochemistry, the positively stained virus particles were found on the epithelial cells of bronchus and alveolus, and also in the endochylema of lymphomonocytes. The specific electophoretic band of 464bp amplified by RT-PCR from samples of pharyngeal swabs, lungs, kidneys, hearts and brains was as same as the theory value. HI antibody titers of the survived cat were 1:32.
authors: Institute of Military Veterinary, Academy of Military Medical Sciences of PLA, Changchun 130062, China.
authors: Shuang
authors: S
authors: Chang
authors: Zhuang
authors: Z
authors: Ding
authors: Song-Tao
authors: ST
authors: Yang
authors: Yu-Wei
authors: YW
authors: Gao
authors: Xiao-Huan
authors: XH
authors: Zou
authors: Tie-Cheng
authors: TC
authors: Wang
authors: Xian-Zhu
authors: XZ
authors: Xia
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002371:Cat Diseases / Q000473:pathology*; D002415:Cats; D006385:Hemagglutination Inhibition Tests; D007150:Immunohistochemistry; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology / Q000472:pathogenicity*; D009976:Orthomyxoviridae Infections / Q000473:pathology* / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046010:Tigers / Q000821:virology*
chemical_list: D000914:Antibodies, Viral