Two closely related pathways of nicotine catabolism in Arthrobacter nicotinovorans and Nocardioides sp. strain JS614.
A virtually identical nicotine catabolic pathway including the heterotrimeric molybdenum enzyme nicotine and 6-hydroxy-pseudo-oxynicotine dehydrogenase, 6-hydroxy-L: -nicotine oxidase, 2,6-dihydroxy-pseudo-oxynicotine hydrolase, and 2,6-dihydroxypyridine hydroxylase have been identified in A. nicotinovorans and Nocardioides sp. JS614. Enzymes catalyzing the same reactions and similar protein antigens were detected in the extracts of the two microorganisms. Nicotine blue and methylamine, two end products of nicotine catabolism were detected in the growth medium of both bacterial species. Nicotine catabolic genes are clustered on pAO1 in A. nicotinovorans, but located chromosomally in Nocardioides sp. JS614.
Ganas
Petra
P
Institute of Biochemistry and Molecular Biology, Centre for Biochemistry and Molecular Cell Biology, Albert-Ludwigs-University Freiburg, Hermann Herder Strasse 7, Freiburg, Germany.
Sachelaru
Paula
P
Mihasan
Marius
M
Igloi
Gabor L
GL
Brandsch
Roderich
R
D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000201:enzymology* / Q000235:genetics; D015153:Blotting, Western; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D006900:Hydroxylation; D008744:Methylamines / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D005810:Multigene Family; D009538:Nicotine / Q000378:metabolism*; D016366:Open Reading Frames; D010957:Plasmids; D011728:Pyridones / Q000378:metabolism
D008744:Methylamines; D011728:Pyridones; C003966:nicotine blue; D009538:Nicotine; D006899:Mixed Function Oxygenases