title: Oxidative stress during acute FIV infection in cats.
abstract: Oxidative stress is thought to contribute to the pathogenesis of HIV infection in humans. For example, CD4(+) T cells are particularly affected in HIV patients and oxidative stress may also contribute to impairment of neutrophil function in HIV/AIDS patients. Since cats infected with FIV develop many of the same immunological abnormalites as HIV-infected humans, we investigated effects of acute FIV infection on oxidative stress in cats. Cats were infected with a pathogenic strain of FIV and viral load, changes in neutrophil number, total blood glutathione, malondiadehye, antioxidant enzyme concentrations, and reduced glutathione (GSH) concentration in leukocytes were measured sequentially during the first 16 weeks of infection. We found that superoxide dismutase and glutathione peroxidase concentrations in whole blood increased significantly during acute FIV infection. In addition, neutrophil numbers increased significantly during this time period, though their intracellular GSH concentrations did not change. In contrast, the numbers of CD4(+) T cells decreased significantly and their intracellular GSH concentration increased significantly, while intracellular GSH concentrations were unchanged in CD8(+) T cells. However, by 16 weeks of infection, many of the abnormalities in oxidative balance had stabilized or returned to pre-inoculation values. These results suggest that acute infection with FIV causes oxidative stress in cats and that CD4(+) T cells appear to be preferentially affected. Further studies are required to determine whether early treatment with anti-oxidants may help ameliorate the decline in CD4(+) T cell number and function associated with acute FIV infection in cats.
authors: Department of Clinical Sciences, Colorado State University, Ft. Collins, CO 80523, USA. cbwebb@colostate.edu
authors: Craig
authors: C
authors: Webb
authors: Tracy
authors: T
authors: Lehman
authors: Kelly
authors: K
authors: McCord
authors: Paul
authors: P
authors: Avery
authors: Steven
authors: S
authors: Dow
mesh_terms: D000208:Acute Disease; D000818:Animals; D002415:Cats; D004912:Erythrocytes / Q000201:enzymology; D016181:Feline Acquired Immunodeficiency Syndrome / Q000378:metabolism* / Q000821:virology; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D015227:Lipid Peroxidation; D009504:Neutrophils / Q000276:immunology; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000097:blood; D013601:T-Lymphocytes / Q000378:metabolism; D019562:Viral Load
chemical_list: D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione