title: Direct selection and phage display of a Gram-positive secretome.
abstract: Surface, secreted and transmembrane protein-encoding open reading frames, collectively the secretome, can be identified in bacterial genome sequences using bioinformatics. However, functional analysis of translated secretomes is possible only if many secretome proteins are expressed and purified individually. We have now developed and applied a phage display system for direct selection, identification, expression and purification of bacterial secretome proteins.
authors: Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand. d.jankovic@massey.ac.nz
authors: Dragana
authors: D
authors: Jankovic
authors: Michael A
authors: MA
authors: Collett
authors: Mark W
authors: MW
authors: Lubbers
authors: Jasna
authors: J
authors: Rakonjac
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D052201:Lacticaseibacillus rhamnosus / Q000737:chemistry* / Q000235:genetics; D016366:Open Reading Frames; D019151:Peptide Library; D021382:Protein Sorting Signals; D040901:Proteomics / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D019151:Peptide Library; D021382:Protein Sorting Signals