Effective production and purification of the glycosylated TSOL18 antigen, which is protective against pig cysticercosis.
Cysticercosis caused by Taenia solium metacestodes is a worldwide public health problem. Important progress in the development of effective and practical vaccines against this disease has been made. In this study, the promising T. solium oncospheral vaccine candidate named TSOL18 antigen was produced in a 5-liter fermentor. During the process of fermentation, the pH of the culture was always kept below 5.0, and in order to prevent foaming, an antifoam agent was added. In addition, the oxygen content of the culture was constantly kept at >50% in our experiment. A high level of the glycosylated protein (2.5 g/liter) was obtained, and the protein was easily purified by gel chromatography. Vaccination trials showed that the recombinant TSOL18 antigen induced 94 and 100% reductions in metacestode burdens in vaccinated pigs, obviously higher than the 89% reduction in pigs immunized with cysticercus crude extracts in trial 1. These are very promising results in the development of an efficient tool to control cysticercosis in Asia.
Cai
Xuepeng
X
Key Laboratory of Zoonoses of CAAS, Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu, China. caixp@public.lz.gs.cn
Yuan
Gailing
G
Zheng
Yadong
Y
Luo
Xuenong
X
Zhang
Shaohua
S
Ding
Juntao
J
Jing
Zhizhong
Z
Lu
Chengping
C
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification*; D019149:Bioreactors; D002850:Chromatography, Gel / Q000379:methods; D003001:Cloning, Molecular; D003470:Culture Media / Q000737:chemistry; D003551:Cysticercosis / Q000517:prevention & control*; D006023:Glycoproteins / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D041201:Taenia solium / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D003470:Culture Media; D006023:Glycoproteins; D011994:Recombinant Proteins; C113407:TSOL-18 antigen