Refolding of denatured lysozyme by water-in-oil microemulsions of sucrose fatty acid esters.
Water-in-oil (w/o) microemulsion of sucrose fatty acid ester was used to renature denatured hen egg white lysozyme without aggregation. After lysozyme was denatured in 5 M guanidine hydrochloride for 24 h, the resultant denatured lysozyme was held in the microemulsion, overnight at 25 degrees C. Renatured lysozyme was transferred from the microemulsion phase to the recovery aqueous phase by conventional liquid-liquid extraction. The enzymatic activity of the recovered lysozyme was 93%.
Noritomi
Hidetaka
H
Division of Applied Chemistry, Tokyo Metropolitan University, Minami-Ohsawa, Hachioji, Tokyo 192-0397, Japan. noritomi@ecomp.metro-u.ac.jp
Takasugi
Tsubasa
T
Kato
Satoru
S
D004655:Emulsions; D004952:Esters; D005227:Fatty Acids / Q000737:chemistry*; D019791:Guanidine / Q000737:chemistry; D008956:Models, Chemical; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism*; D009821:Oils / Q000737:chemistry*; D011489:Protein Denaturation; D017510:Protein Folding; D013395:Sucrose / Q000737:chemistry; D014867:Water / Q000737:chemistry*
D004655:Emulsions; D004952:Esters; D005227:Fatty Acids; D009821:Oils; D014867:Water; D013395:Sucrose; C421864:hen egg lysozyme; D009113:Muramidase; D019791:Guanidine