title: Differential localization of GABAA receptor subunits within the substantia nigra of the human brain: an immunohistochemical study.
abstract: Gamma-aminobutyric acid(A) (GABA(A)) receptors (GABA(A)R) are inhibitory heteropentameric chloride ion channels comprising a variety of subunits and are localized at postsynaptic sites within the central nervous system. In this study we present the first detailed immunohistochemical investigation on the regional, cellular, and subcellular localisation of alpha(1), alpha(2), alpha(3), beta(2,3), and gamma(2) subunits of the GABA(A)R in the human substantia nigra (SN). The SN comprises two major regions, the SN pars compacta (SNc) consisting of dopaminergic projection neurons, and the SN pars reticulata (SNr) consisting of GABAergic parvalbumin-positive projection neurons. The results of our single- and double-labeling studies demonstrate that in the SNr GABA(A) receptors contain alpha(1), alpha(3), beta(2,3), and gamma(2) subunits and are localized in a weblike network over the cell soma, dendrites, and spines of SNr parvalbumin-positive nonpigmented neurons. By contrast, GABA(A)Rs on the SNc dopaminergic pigmented neurons contain predominantly alpha(3) and gamma(2) subunits; however there is GABA(A)R heterogeneity in the SNc, with a small subpopulation (6.5%) of pigmented SNc neurons additionally containing alpha(1) and beta(2,3) GABA(A)R subunits. Also, in the SNr, parvalbumin-positive terminals are adjacent to GABA(A)R on the soma and proximal dendrites of SNr neurons, whereas linear arrangements of substance P-positive terminals are adjacent to GABA(A) receptors on all regions of the dendritic tree. These results show marked GABA(A)R subunit hetereogeneity in the SN, suggesting that GABA exerts quite different effects on pars compacta and pars reticulata neurons in the human SN via GABA(A) receptors of different subunit configurations.
authors: Department of Anatomy with Radiology, Faculty of Medical and Health Science, University of Auckland, Auckland 1142, New Zealand. h.waldvogel@auckland.ac.nz
authors: H J
authors: HJ
authors: Waldvogel
authors: K
authors: K
authors: Baer
authors: W-P
authors: WP
authors: Gai
authors: R T
authors: RT
authors: Gilbert
authors: M I
authors: MI
authors: Rees
authors: H
authors: H
authors: Mohler
authors: R L M
authors: RL
authors: Faull
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000145:classification / Q000166:cytology / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism; D011180:Postmortem Changes; D021122:Protein Subunits / Q000378:metabolism; D011963:Receptors, GABA-A / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism; D013378:Substantia Nigra / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D010320:Parvalbumins; D021122:Protein Subunits; D011963:Receptors, GABA-A; D013373:Substance P; D014446:Tyrosine 3-Monooxygenase