A microwave technique for double indirect immunostaining of human brain tissue cultures with mouse monoclonal antibodies.
The use of 2 monoclonal antibodies during double immunohistochemistry would enable the use of a greater variety of antibody combinations. Here, we demonstrate a simple, cost effective method of double indirect immunostaining of cultured cells using primary antibodies from the same species. This method uses microwaving of cell samples immediately after the application of the first secondary antibody, and significantly reduces the level of nonspecific staining. This technique does not elute the antibodies, nor raise the sample temperature above 37 degrees C.
Jufas
Nicholas E
NE
Department of Medicine, University of Sydney, NSW, Australia.
Roediger
Ben
B
Armati
Patricia J
PJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D001923:Brain Chemistry; D002478:Cells, Cultured; D005333:Fetus; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D008872:Microwaves; D014404:Tubulin / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D005904:Glial Fibrillary Acidic Protein; D014404:Tubulin