title: Molecular method for Bartonella species identification in clinical and environmental samples.
abstract: A new, efficient molecular method for detection of Bartonella, based on the 16S-23S rRNA intergenic spacer and 16S rRNA amplification by multiplex PCR combined with reverse line blotting, was designed. This assay could simultaneously detect 20 different known species and other Bartonella species not described previously.
authors: Laboratorio de Espiroquetas y Patógenos Especiales, Servicio de Bacteriología, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain.
authors: Coral
authors: C
authors: García-Esteban
authors: Horacio
authors: H
authors: Gil
authors: Manuela
authors: M
authors: Rodríguez-Vargas
authors: Xeider
authors: X
authors: Gerrikagoitia
authors: Jesse
authors: J
authors: Barandika
authors: Raquel
authors: R
authors: Escudero
authors: Isabel
authors: I
authors: Jado
authors: Cristina
authors: C
authors: García-Amil
authors: Marta
authors: M
authors: Barral
authors: Ana L
authors: AL
authors: García-Pérez
authors: Mangesh
authors: M
authors: Bhide
authors: Pedro
authors: P
authors: Anda
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D001473:Bartonella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001474:Bartonella Infections / Q000175:diagnosis*; D017931:DNA Primers / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers; D021903:DNA, Ribosomal Spacer; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S