Prevention of osteoporosis and hypogonadism by allogeneic ovarian transplantation in conjunction with intra-bone marrow-bone marrow transplantation.
BACKGROUND
We investigated the effects of ovarian allograft in conjunction with intra-bone marrow-bone marrow transplantation (IBM-BMT) on estrogen deficiency in mice.


METHODS
Female C57BL/6 mice underwent ovariectomy (OvX). After 3 months, the mice were irradiated at 9.5 Gy, and the bone marrow cells (BMCs) of female BALB/c mice (8 weeks old) were then injected into the bone cavity of the B6 mice. Simultaneously, allogeneic ovaries from BALB/c mice were transplanted under the renal capsules of the B6 mice.


RESULTS
Three months after the transplantation, the hematolymphoid cells were found to be completely reconstituted with donor-derived cells. The transplanted ovary tissues under the renal capsules were accepted without using immunosuppressants; there were a large number of growing follicles at different stages of development. Atrophic endometrium and its glands were also recovered by ovarian transplantation (OT). The transplanted allogeneic ovaries secreted estrogen at normal levels. Furthermore, bone loss was prevented to a certain extent.


CONCLUSIONS
These findings suggest that IBM-BMT+OT will become a valuable strategy for young women with malignant tumors to prevent premature senescence, including hypogonadism and osteoporosis, after radiochemotherapy.
Feng
Wei
W
First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, Japan.
Cui
Yunze
Y
Song
Changye
C
Zhan
Hongsheng
H
Wang
Xiaoli
X
Li
Qing
Q
Cui
Wenhao
W
Guo
Kequan
K
Maki
Masahiko
M
Hisha
Hiroko
H
Mori
Takahide
T
Ikehara
Susumu
S
D000596:Amino Acids / Q000652:urine; D000818:Animals; D000941:Antigens / Q000276:immunology; D016026:Bone Marrow Transplantation / Q000276:immunology; D004958:Estradiol / Q000097:blood; D005260:Female; D007006:Hypogonadism / Q000097:blood / Q000276:immunology / Q000517:prevention & control* / Q000652:urine; D051379:Mice; D009929:Organ Size; D010024:Osteoporosis / Q000097:blood / Q000276:immunology / Q000517:prevention & control* / Q000652:urine; D010053:Ovary / Q000276:immunology / Q000637:transplantation*; D014184:Transplantation, Homologous / Q000276:immunology
D000596:Amino Acids; D000941:Antigens; D004958:Estradiol; C036020:deoxypyridinoline