title: Evaluation of the immunomodulatory activities of royal jelly components in vitro.
abstract: In this work the effect of different components isolated from royal jelly (RJ) was studied using an in vitro rat T-cell proliferation assay. We found that lower concentrations of MEL 174 (final water extract of RJ) and MEL 147 (3-10-dihydroxydecanoic acid) stimulated T-cell proliferation, triggered by concanavalin A (Con-A) and the process was followed by an increase in the production of interleukin-2 (IL-2). Higher concentrations of MEL 174, MEL 247 (dry powder of RJ) and MEL 138 (trans-10-hydroxydec-2-enoic acid) inhibited T-cell proliferation. The inhibition of T-cell proliferation in the presence of MEL 174 was followed by a decrease in IL-2 production, which was partly abrogated by exogenous IL-2, a decrease in nitric oxide (NO) production and increased apoptosis. In conclusion, our results showed the complexity of biological activity of RJ and suggest that its water extract possesses the most potent immunomodulatory activity in vitro.
authors: Institute of Medical Research, MMA, Belgrade, Crnotravska, Belgrade, Serbia.
authors: Sonja
authors: S
authors: Gasic
authors: Dragana
authors: D
authors: Vucevic
authors: Sasa
authors: S
authors: Vasilijic
authors: Mirjana
authors: M
authors: Antunovic
authors: Ioanna
authors: I
authors: Chinou
authors: Miodrag
authors: M
authors: Colic
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003208:Concanavalin A; D005227:Fatty Acids / Q000737:chemistry* / Q000494:pharmacology*; D007155:Immunologic Factors; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000032:analysis / Q000378:metabolism; D008297:Male; D000432:Methanol; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000096:biosynthesis; D051381:Rats; D012997:Solvents; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects; D000099219:Royal Jelly
chemical_list: D003208:Concanavalin A; D005227:Fatty Acids; D007155:Immunologic Factors; D007376:Interleukin-2; D000432:Methanol; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide; D012997:Solvents; D000099219:Royal Jelly