title: [Expression, subcellular localization and nuclear translocation of transcription factor up stream stimulatory factor-1 in odontoblasts].
abstract: OBJECTIVE
  To examine the expression and subcellular localization of transcription factor USF1 in odontoblasts and investigate whether nuclear translocation occurs under stimuli.
  
  
  METHODS
  Odontoblasts MDPC-23 were cultured on coverslips and divided into 2 groups. Group 1 received no stimuli, and group 2 was stimulated by nicotine with various concentrations respectively for 1h. Then the mountings of odontoblasts were prepared and immunocytochemical staining was performed with specific USF1 antibody via SABC method. Hela cells were used as positive control.
  
  
  RESULTS
  The staining was positive in the cytoplasm of odontoblasts in group 1, but in the nuclei of Hela cells and in 100 mg/L nicotine-stimulated odontoblasts in group 2.
  
  
  CONCLUSIONS
  There exists USF1 protein in odontoblasts, which locates in the cytoplasm and could translocate into nuclei under the stimulation of nicotine.
authors: Department of Pediatric Dentistry, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
authors: Li-An
authors: LA
authors: Wu
authors: Ling-Ying
authors: LY
authors: Wen
authors: Fu-Sheng
authors: FS
authors: Yang
authors: Xiao-Jing
authors: XJ
authors: Wang
authors: Jun
authors: J
authors: Fang
mesh_terms: D002478:Cells, Cultured; D006367:HeLa Cells; D006801:Humans; D009538:Nicotine / Q000494:pharmacology; D009804:Odontoblasts / Q000187:drug effects / Q000378:metabolism*; D021382:Protein Sorting Signals; D021381:Protein Transport / Q000187:drug effects; D051720:Upstream Stimulatory Factors / Q000378:metabolism*
chemical_list: D021382:Protein Sorting Signals; C497181:USF1 protein, human; D051720:Upstream Stimulatory Factors; D009538:Nicotine