Daily variations in oligosaccharides of human milk determined by microfluidic chips and mass spectrometry.
Human milk is a complex biological fluid that provides not only primary nourishment for infants but also protection against pathogens and influences their metabolic, immunologic, and even cognitive development. The presence of oligosaccharides in remarkable abundance in human milk has been associated to provide diverse biological functions including directing the development of an infant's intestinal microflora and immune system. Recent advances in analytical tools offer invaluable insights in understanding the specific functions and health benefits these biomolecules impart to infants. Oligosaccharides in human milk samples obtained from five different individual donors over the course of a 3 month lactation period were isolated and analyzed using HPLC-Chip/TOF-MS technology. The levels and compositions of oligosaccharides in human milk were investigated from five individual donors. Comparison of HPLC-Chip/TOF-MS oligosaccharides profiles revealed heterogeneity among multiple individuals with no significant variations at different stages of lactation within individual donors.
Niñonuevo
Milady R
MR
Department of Chemistry, University of California, Davis, CA 95616, USA.
Perkins
Patrick D
PD
Francis
Jimi
J
Lamotte
Latasha M
LM
LoCascio
Riccardo G
RG
Freeman
Samara L
SL
Mills
David A
DA
German
J Bruce
JB
Grimm
Rudolf
R
Lebrilla
Carlito B
CB
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D005643:Fucose / Q000032:analysis; D006801:Humans; D007774:Lactation; D013058:Mass Spectrometry; D046210:Microfluidic Analytical Techniques; D008895:Milk, Human / Q000737:chemistry*; D019158:N-Acetylneuraminic Acid / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis*; D013997:Time Factors
D009844:Oligosaccharides; D005643:Fucose; D019158:N-Acetylneuraminic Acid