A pyrimidopyrimidoindole nucleoside (dC PPI): photophysical properties and thermal stability of the modified DNA duplexes.
A new fluorescent deoxycytidine analog, 10-(2-deoxy-beta -D-ribofuranosyl)-pyrimido[4',5' :4,5]-pyrimido[1,6-a]indole-6,9(7H)-dione (dC(PPI)) was synthesized. Its fluorescent properties were studied in detail. It was found that this fluorescent nucleoside dC(PPI) could be used as a fluorescent label for DNA probes with minimal disturbance of their overall structure.
Mizuta
Masahiro
M
CREST, Japan Science and Technology Agency, Yokohama, Japan.
Seio
Kohji
K
Miyata
Kenichi
K
Ohkubo
Akihiro
A
Taguchi
Haruhiko
H
Sekine
Mitsuo
M
D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D003841:Deoxycytidine / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006358:Hot Temperature; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D012265:Ribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*
C527048:10-(2-deoxy-beta -D-ribofuranosyl)-pyrimido(4',5' :4,5)-pyrimido(1,6-a)indole-6,9(7H)-dione; D015342:DNA Probes; D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D012265:Ribonucleotides; D003841:Deoxycytidine; D004247:DNA