Novel MHC class I-related molecule MR1 affects MHC class I expression in 293T cells.
Association with beta(2)-microglobulin and binding a ligand are necessary conditions for cell surface expression of the antigen presenting molecules. MHC class I-related protein, MR1, is suggested to have an antigen presentation function, nevertheless the physiological ligand(s) is (are) still to be determined. In the present study, by characterising the subcellular deportment of human MR1 transfectants, we have shown its differential mobilisation. Our results demonstrated a preferential association of MR1 with beta(2)-microglobulin in MHC class I-deficient B cell lines. Furthermore, we have evidenced diminished expression of classical MHC class I molecules in human MR1-transfected 293T cells, showing a possible interaction between MR1 and classical MHC class I molecules.
Aldemir
Hatice
H
Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Valbonne 06560, France.
D001402:B-Lymphocytes / Q000378:metabolism*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000502:physiology*; D015234:HLA-A Antigens / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D015778:Minor Histocompatibility Antigens; D021381:Protein Transport / Q000502:physiology*; D001613:beta 2-Microglobulin / Q000378:metabolism*
D015234:HLA-A Antigens; D015395:Histocompatibility Antigens Class I; C094840:MR1 protein, human; D015778:Minor Histocompatibility Antigens; D001613:beta 2-Microglobulin