title: Nonenzymatically glycated lipoprotein ApoA-I in plasma of diabetic and nephropathic patients.
abstract: ApoA-I, which constitutes 70% of the apolipoprotein content of high-density lipoproteins, acts as an acceptor for the transfer of phospholipids and free cholesterol from peripheral tissues and transports cholesterol in the liver and other tissue for excretion and steroidogenesis. In order to verify its possible structural alteration in pathological states, plasma samples from healthy, diabetic, and nephropathic subjects have been analyzed by two-dimensional gel electrophoresis. By this approach, clear differences among the three classes of subjects become evident and, in the case of diabetic and nephropathic patients, intense spots are present. The matrix-assisted laser desorption/ionization mass spectrometry analysis of their digestion products shows that an overexpression of unglycated and glycated ApoA-I is present in both pathological states, reasonably affecting the efficiency in cholesterol transport.
authors: Dipartimento di Scienze Mediche e Chirurgiche, Cattedra di Malattie del Metabolismo, Università di Padova, Padova, Italy. annunziata.lapolla@unipd.it
authors: Annunziata
authors: A
authors: Lapolla
authors: Maura
authors: M
authors: Brioschi
authors: Cristina
authors: C
authors: Banfi
authors: Elena
authors: E
authors: Tremoli
authors: Chiara
authors: C
authors: Cosma
authors: Luciana
authors: L
authors: Bonfante
authors: Simone
authors: S
authors: Cristoni
authors: Roberta
authors: R
authors: Seraglia
authors: Pietro
authors: P
authors: Traldi
mesh_terms: D016632:Apolipoprotein A-I / Q000097:blood* / Q000302:isolation & purification; D001786:Blood Glucose / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood / Q000302:isolation & purification; D003404:Creatinine / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D003928:Diabetic Nephropathies / Q000097:blood*; D015180:Electrophoresis, Gel, Two-Dimensional; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014280:Triglycerides / Q000097:blood
chemical_list: D016632:Apolipoprotein A-I; D001786:Blood Glucose; D002784:Cholesterol; D008076:Cholesterol, HDL; D003404:Creatinine; D006442:Glycated Hemoglobin; D014280:Triglycerides