title: Effects of high-yield thrombocytapheresis on the quality of platelet products.
abstract: BACKGROUND
  The steadily increasing demands for single-donor apheresis platelet (PLT) concentrates (APCs) are a challenge to the PLT supply system. Therefore, efforts to improve plateletpheresis yield, allowing apheresis products to be split into 2 or more units, are valuable strategies. No data to demonstrate in vivo transfusion efficacy of these high-yield split-APCs are currently available, however.
  
  
  METHODS
  The transfusion efficacy of APCs produced by two apheresis methods involving different harvest and storing procedures and varying PLT yields was investigated. Efficacy measures were the 1-hour percent PLT recovery (PPR(1h)) and the 1-hour corrected count increment (CCI(1h)). In total, 400 APCs, produced with either an Amicus device (Baxter) and stored in PLT additive solution (T-Sol; Amicus method [AM], n = 107) or a Trima device (Gambro) and stored in plasma (Trima method [TM], n = 293), were transfused to 55 children (31 girls; median age, 9.5 years; range, 0.2-18.5 years) with thrombocytopenia due to chemotherapy or aplastic anemia (median, 4 APCs per child; range, 1-68).
  
  
  RESULTS
  Transfusion efficacy was significantly lower for AM-APCs than for TM-APCs (median PPR(1h), 17 and 33%; median CCI(1h), 7.9 and 15.6, respectively; p < 0.001). Reduced transfusion efficacy correlated in a yield-dependent manner with high apheresis PLT yields (> or =6 x 10(11)) for AM-APCs (p < 0.001).
  
  
  CONCLUSIONS
  Although in vitro validation of AM- and TM-APCs has been performed, only by evaluating transfusion efficacy in vivo did the AM turn out to be not suitable for high-yield thrombocytapheresis. This study recommends the implementation of in vivo transfusion efficacy studies for high-yield APC apheresis donations.
authors: Department of Pediatrics, University of Berne, Berne, Switzerland.
authors: Friedgard
authors: F
authors: Julmy
authors: Roland A
authors: RA
authors: Ammann
authors: Behrouz
authors: B
authors: Mansouri Taleghani
authors: Stefano
authors: S
authors: Fontana
authors: Andreas
authors: A
authors: Hirt
authors: Kurt
authors: K
authors: Leibundgut
mesh_terms: D000293:Adolescent; D000741:Anemia, Aplastic / Q000097:blood / Q000628:therapy; D001792:Blood Platelets / Q000166:cytology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D010976:Platelet Count; D017713:Platelet Transfusion / Q000379:methods; D010983:Plateletpheresis / Q000379:methods*; D013921:Thrombocytopenia / Q000097:blood / Q000628:therapy; D016896:Treatment Outcome