Signal processes and ROS production in glucose transport regulation by thrombopoietin and granulocyte macrophage-colony stimulation factor in a human leukaemic cell line.
In M07e cells, a human megakaryocytic leukaemia line, reactive oxygen species (ROS) are generated in response to cytokines acting as intracellular messengers to modulate glucose transport. The aim of this work was to study the signal cascade involved in the acute glucose transport activation in cells exposed to growth factors, such as granulocyte macrophage-colony stimulation factor (GM-CSF) and thrombopoietin (TPO), to better understand some aspects of the aberrant proliferation in leukaemia. Results confirm ROS involvement in modulation of glucose transport in this cell line. Furthermore, GM-CSF and TPO produced changes in Glut1 phosphorylation and specific inhibitors employed to identify protein kinases involved in Glut activation by these cytokines proved that Akt, PLC gamma, Syk and the Src family take part in signal transduction leading to Glut1 activation.
Maraldi
Tullia
T
Department of Biochemistry G. Moruzzi, University of Bologna, Bologna, Italy. tullia.maraldi@unibo.it
Prata
Cecilia
C
Fiorentini
Diana
D
Zambonin
Laura
L
Landi
Laura
L
Hakim
Gabriele
G
D001692:Biological Transport / Q000187:drug effects; D045744:Cell Line, Tumor; D004963:Estrenes / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D007947:Leukemia, Megakaryoblastic, Acute; D011760:Pyrrolidinones / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction; D019284:Thapsigargin / Q000494:pharmacology; D013926:Thrombopoietin / Q000494:pharmacology*
D004963:Estrenes; D011760:Pyrrolidinones; D017382:Reactive Oxygen Species; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; D019284:Thapsigargin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D013926:Thrombopoietin; D005947:Glucose