title: Platelet-derived stromal cell-derived factor-1 regulates adhesion and promotes differentiation of human CD34+ cells to endothelial progenitor cells.
abstract: BACKGROUND
  Peripheral homing of progenitor cells in areas of diseased organs is critical for tissue regeneration. The chemokine stromal cell-derived factor-1 (SDF-1) regulates homing of CD34+ stem cells. We evaluated the role of platelet-derived SDF-1 in adhesion and differentiation of human CD34+ cells into endothelial progenitor cells.
  
  
  RESULTS
  Adherent platelets express substantial amounts of SDF-1 and recruit CD34+ cells in vitro and in vivo. A monoclonal antibody to SDF-1 or to its counterreceptor, CXCR4, inhibits stem cell adhesion on adherent platelets under high arterial shear in vitro and after carotid ligation in mice, as determined by intravital fluorescence microscopy. Platelets that adhere to human arterial endothelial cells enhance the adhesion of CD34+ cells on endothelium under flow conditions, a process that is inhibited by anti-SDF-1. During intestinal ischemia/reperfusion in mice, anti-SDF-1 and anti-CXCR4, but not isotype control antibodies, abolish the recruitment of CD34+ cells in microcirculation. Moreover, platelet-derived SDF-1 binding to CXCR4 receptor promotes platelet-induced differentiation of CD34+ cells into endothelial progenitor cells, as verified by colony-forming assays in vitro.
  
  
  CONCLUSIONS
  These findings imply that platelet-derived SDF-1 regulates adhesion of stem cells in vitro and in vivo and promotes differentiation of CD34+ cells to endothelial progenitor cells. Because tissue regeneration depends on recruitment of progenitor cells to peripheral vasculature and their subsequent differentiation, platelet-derived SDF-1 may contribute to vascular and myocardial regeneration.
authors: Medizinische Klinik III, Eberhard Karls-Universitätsklinikum Tübingen, Tübingen, Germany.
authors: Konstantinos
authors: K
authors: Stellos
authors: Harald
authors: H
authors: Langer
authors: Karin
authors: K
authors: Daub
authors: Tanja
authors: T
authors: Schoenberger
authors: Alexandra
authors: A
authors: Gauss
authors: Tobias
authors: T
authors: Geisler
authors: Boris
authors: B
authors: Bigalke
authors: Iris
authors: I
authors: Mueller
authors: Michael
authors: M
authors: Schumm
authors: Iris
authors: I
authors: Schaefer
authors: Peter
authors: P
authors: Seizer
authors: Bjoern F
authors: BF
authors: Kraemer
authors: Dorothea
authors: D
authors: Siegel-Axel
authors: Andreas E
authors: AE
authors: May
authors: Stephan
authors: S
authors: Lindemann
authors: Meinrad
authors: M
authors: Gawaz
mesh_terms: D000818:Animals; D018952:Antigens, CD34; D001792:Blood Platelets / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000502:physiology*; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D051379:Mice; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology*
chemical_list: D018952:Antigens, CD34; D054377:Chemokine CXCL12