title: In vitro cytotoxicity of dental alloys and cpTi obtained by casting.
abstract: BACKGROUND
  This study aimed to compare the cytotoxicity of base-metal dental alloys and to evaluate if the casting method could influence their cytotoxicity.
  
  
  METHODS
  Disks of base-metal dental alloys were cast by two methods: plasma, under argon atmosphere, injected by vacuum-pressure; and oxygen-gas flame, injected by centrifugation, except Ti-6Al-4V and commercially pure titanium (cpTi), cast only by plasma. SCC9 cells were cultured in culture media D-MEM/Ham's F12 supplemented, at 37 degrees C in a humidified atmosphere of 5% carbon dioxide and 95% air, on the previously prepared disks. At subconfluence in wells without disks (control), cell number and viability were evaluated.
  
  
  RESULTS
  In plasma method, cpTi and Ti-6Al-4V were similar to control and presented higher number of cells than all other alloys, followed by Ni-Cr. In oxygen-gas flame method, all alloys presented fewer cells than control. Ni-Cr presented more cells than any other alloy, followed by Co-Cr-Mo-W which presented more cells than Ni-Cr-Ti, Co-Cr-Mo, and Ni-Cr-Be. There were no significant differences between casting methods related to cell number. Cell viability was not affected by either chemical composition or casting methods.
  
  
  CONCLUSIONS
  cpTi and Ti-6Al-4V were not cytotoxic while Ni-Cr-Be was the most cytotoxic among tested alloys. The casting method did not affect cytotoxicity of the alloys.
authors: Department of Dental Materials and Prostheses, Dental School of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
authors: Adriana C L
authors: AC
authors: Faria
authors: Adalberto L
authors: AL
authors: Rosa
authors: Renata C S
authors: RC
authors: Rodrigues
authors: Ricardo F
authors: RF
authors: Ribeiro
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D003343:Corrosion; D003722:Dental Alloys / Q000009:adverse effects*; D003735:Dental Casting Technique; D006801:Humans; D008422:Materials Testing / Q000379:methods
chemical_list: D003722:Dental Alloys