[Study on purification of total flavonoids from Rosa laevigata with macroporous resin column chromatography].
OBJECTIVE
To Study the technologic conditions of purifying total Rosa laevigata by AB-8 pocket.


METHODS
Extracting flavonoids from Rosa laevigata with 70% alcohol, the thick flavonoid powder were obtained and the content of pure flavonoids in it could be determinated using spectrophotometry. Taking the adsorption of the AB-8 pocket to Rosa flavonoids as the research object, the factors that affected the AB-8 resin static absorption and desorption were studied preliminarily.


RESULTS
The result showed that the effect was perfect when the flavononid concentration in the liquid was 0.3 mg/ml, the loading amount was 120 mg with the adsorption-power 1 ml/ min; And the volume of 70% alcohol was eluant 5 BV with desorption-power 1 ml/min. The pure flavonoid content in thick flavonoid powder was 25.2% and it reached 42.2% after being purified by the AB-8 pocket adsorption resin.


CONCLUSIONS
It proved AB-8 pocket adsorption resin have a better effect on purification of the flavononids of Rosa laevigata.
Chen
Nai-fu
NF
Department of Chemistry and Life Science, West of Anhui University, Liuan 237012, China.
Chen
Ke
K
Zhang
Li
L
Yang
Huai-zhi
HZ
D000327:Adsorption; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D000431:Ethanol; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification*; D005638:Fruit / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D015203:Reproducibility of Results; D012117:Resins, Synthetic / Q000737:chemistry*; D019685:Rosales / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods
D005419:Flavonoids; D012117:Resins, Synthetic; D000431:Ethanol