title: Assembly and structural analysis of a covalently closed nano-scale DNA cage.
abstract: The inherent properties of DNA as a stable polymer with unique affinity for partner molecules determined by the specific Watson-Crick base pairing makes it an ideal component in self-assembling structures. This has been exploited for decades in the design of a variety of artificial substrates for investigations of DNA-interacting enzymes. More recently, strategies for synthesis of more complex two-dimensional (2D) and 3D DNA structures have emerged. However, the building of such structures is still in progress and more experiences from different research groups and different fields of expertise are necessary before complex DNA structures can be routinely designed for the use in basal science and/or biotechnology. Here we present the design, construction and structural analysis of a covalently closed and stable 3D DNA structure with the connectivity of an octahedron, as defined by the double-stranded DNA helices that assembles from eight oligonucleotides with a yield of approximately 30%. As demonstrated by Small Angle X-ray Scattering and cryo-Transmission Electron Microscopy analyses the eight-stranded DNA structure has a central cavity larger than the apertures in the surrounding DNA lattice and can be described as a nano-scale DNA cage, Hence, in theory it could hold proteins or other bio-molecules to enable their investigation in certain harmful environments or even allow their organization into higher order structures.
authors: Department of Molecular Biology and Interdisciplinary Nanoscience Center (iNANO), University of Aarhus, C.F. Møllers Allé, Bldg. 1130, 8000 Aarhus C, Denmark.
authors: Felicie F
authors: FF
authors: Andersen
authors: Bjarne
authors: B
authors: Knudsen
authors: Cristiano Luis Pinto
authors: CL
authors: Oliveira
authors: Rikke F
authors: RF
authors: Frøhlich
authors: Dinna
authors: D
authors: Krüger
authors: Jörg
authors: J
authors: Bungert
authors: Mavis
authors: M
authors: Agbandje-McKenna
authors: Robert
authors: R
authors: McKenna
authors: Sissel
authors: S
authors: Juul
authors: Christopher
authors: C
authors: Veigaard
authors: Jørn
authors: J
authors: Koch
authors: John L
authors: JL
authors: Rubinstein
authors: Bernt
authors: B
authors: Guldbrandtsen
authors: Marianne S
authors: MS
authors: Hede
authors: Göran
authors: G
authors: Karlsson
authors: Anni H
authors: AH
authors: Andersen
authors: Jan Skov
authors: JS
authors: Pedersen
authors: Birgitta R
authors: BR
authors: Knudsen
mesh_terms: D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D046529:Microscopy, Electron, Transmission; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
chemical_list: D009841:Oligonucleotides; D004247:DNA