title: Expression of in vivo-inducible Salmonella enterica promoters during infection of Caenorhabditis elegans.
abstract: In vitro mimicking of the stimuli controlling in vivo-inducible bacterial promoters during infection of the host can be complex. Therefore, the use of the nematode Caenorhabditis elegans was evaluated, as a surrogate host to examine the expression of Salmonella enterica promoters. Green fluorescent protein (GFP+) was put under the control of the promoters of the pagC, mgtB, sseA, pgtE and fur genes of S. enterica. After infection of C. elegans with an S. enterica serovar Typhimurium vaccine strain expressing these constructs, clear bacterial expression of GFP+ was observed under the control of all five promoters, although significant expression was not always obtained in vitro. It is concluded that C. elegans constitutes a useful model system for the study of the in vivo expression of Salmonella promoters.
authors: Nani
authors: N
authors: Van Gerven
authors: Veerle
authors: V
authors: Derous
authors: Jean-Pierre
authors: JP
authors: Hernalsteens
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D017173:Caenorhabditis elegans / Q000382:microbiology*; D027682:Cation Transport Proteins / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics; D008856:Microscopy, Fluorescence; D018832:Molecular Chaperones / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics; D019779:Salmonella enterica / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; D012097:Repressor Proteins; C474474:SseA protein, Salmonella typhimurium; C068477:ferric uptake regulating proteins, bacterial; C066766:pagC protein, Salmonella typhimurium; D049452:Green Fluorescent Proteins; D010450:Endopeptidases; C411337:PgtE protein, Salmonella enterica; D000251:Adenosine Triphosphatases; C083836:MgtB protein, Salmonella typhimurium