title: Identical rearrangement of NSP3 genes found in three independently isolated virus clones derived from mixed infection and multiple passages of Rotaviruses.
abstract: Three rotavirus variants with a rearranged RNA segment derived from the NSP3 gene were isolated in three independent experiments of coinfection and multiple passages of simian rotavirus strain SA11 and single-VP7-gene- or NSP1-gene-substitution reassortants having genetic background of SA11. Sequence analysis indicated that the three rearranged NSP3 genes had almost identical sequences and genomic structures organized by partial duplication of the open reading frame in a head-to-tail orientation following the termination codon. The junction site of the original NSP3 gene (first copy) and the duplicated portion (second copy) was identical among the three rearranged genes, while a direct repeat, i.e., a homologous sequence between the first copy and second template for duplication, typically located at the junction site, was not detected. However, short similar sequences were present at the end of the first copy and beginning of the second copy. These findings suggest that rearrangement of the NSP3 gene may occur at a certain preferential site which is related to sequence similarity between 3'-untranslated region and a region near the 5'-end of ORF.
authors: Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
authors: M M
authors: MM
authors: Alam
authors: N
authors: N
authors: Kobayashi
authors: M
authors: M
authors: Ishino
authors: S
authors: S
authors: Nagashima
authors: S K
authors: SK
authors: Paul
authors: M
authors: M
authors: Chawla-Sarkar
authors: T
authors: T
authors: Krishnan
authors: T N
authors: TN
authors: Naik
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D015321:Gene Rearrangement; D005814:Genes, Viral; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification; D017423:Sequence Analysis, RNA; D012692:Serial Passage; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: C082740:NSP3 protein, Rotavirus; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins