title: [Effect of oral carcinoma-associated fibroblasts on extracellular signal-regulated kinases pathway in the lingual carcinoma cell line].
abstract: OBJECTIVE
  To investigate the effects of oral carcinoma-associated fibroblasts (CAFs) on extracellular signal-regulated kinases (ERK) pathway in a lingual carcinoma cell line.
  
  
  METHODS
  The lingual carcinoma cell line, Tca8113 was stimulated by conditioned medium from oral CAFs, or cocultured with oral CAFs for definite time. Total ERK and pERK in Tca8113 lysate were detected by Western blotting, and the ratio between pERK and ERK were calculated.
  
  
  RESULTS
  Both stimulation by conditioned medium and coculture induced prompt phosphorylation of ERK, and increased the ratio between pERK and ERK.
  
  
  CONCLUSIONS
  Oral CAFs can activate ERK pathway of carcinoma cells.
authors: Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
authors: Jing-wen
authors: JW
authors: Lin
authors: Qian-ming
authors: QM
authors: Chen
authors: Sheng-fu
authors: SF
authors: Li
authors: Chun-hua
authors: CH
authors: Fu
authors: Mei
authors: M
authors: Lin
authors: Dan
authors: D
authors: Long
authors: Hong-mei
authors: HM
authors: Zhou
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D048049:Extracellular Signal-Regulated MAP Kinases; D005347:Fibroblasts; D006801:Humans; D009062:Mouth Neoplasms; D010766:Phosphorylation; D014062:Tongue Neoplasms
chemical_list: D048049:Extracellular Signal-Regulated MAP Kinases