Characterization of highly concentrated serum lectins in spotted halibut Verasper variegatus.
Mannose-binding lectins were purified from flatfish spotted halibut (Verasper variegatus) serum. These lectins, which we named VVL (Verasper variegatus lectin)-alpha (approximately 33 kDa) and VVL-beta (approximately 30 kDa) (VVLs), under non-reducing SDS-PAGE, were surprisingly highly concentrated in serum (1.92+/-0.55 mg/ml; n=5), compared with other serum lectins. Both VVLs are heterodimers comprised of 2 types of subunit via inter-subunit disulfide bonds, and one subunit of VVL-alpha has a N-linked sugar chain. Based on N-terminal amino acid sequences, the nucleotide sequences of one subunit of VVL cDNAs were determined by 3'- and 5'-rapid amplification of the cDNA ends. The full-length VVL subunit cDNAs contained 489 bp, encoding an open reading frame of 163 amino acids. We found that VVLs bind to an approximately 8 kDa ciliary surface glycoprotein (a putative agglutination/immobilization antigen that we reported previously) of the fish parasite Neobenedenia girellae, and agglutinate this parasite in vitro.
Hatanaka
A
A
Central Research Laboratories of Nippon Suisan Kaisha Ltd, 559-6 Kitano-Machi, Hachioji, Tokyo 192-0906, Japan. hatanaka@nissui.co.jp
Umeda
N
N
Hirazawa
N
N
D000371:Agglutination; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002923:Cilia / Q000378:metabolism; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D005432:Flounder / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D005784:Gene Amplification; D006023:Glycoproteins; D006386:Hemagglutination Tests; D037102:Lectins / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010986:Platyhelminths / Q000276:immunology* / Q000378:metabolism
D018076:DNA, Complementary; D006023:Glycoproteins; D037102:Lectins