A portal vein cannulation technique for drug discovery in mice.
One approach to understanding how orally administered drugs are absorbed and metabolized involves measuring compound concentrations in portal vein blood and in systemic circulation at various time points. In mice, blood samples are generally collected through terminal bleeding, a process that requires a large number of mice and is susceptible to variation between individuals. The authors developed a portal vein cannulation procedure for serial bleeding in the mouse, using a modified catheter containing a stainless steel stylet that is implanted directly in the portal vein. To demonstrate the technique, they orally administered two different compounds to mice and obtained blood samples from the tail vein and portal vein at different time points. They analyzed compound concentrations using liquid chromatography-tandem mass spectrometry. The technique refines existing methods for pharmacokinetic studies in the mouse and reduces the number of mice required.
Shen
Xiaolan
X
Department of Laboratory Animal Resources, Merck Research Laboratories, PO Box 2000, RY80B-100, Rahway, NJ 07065, USA. xiaolan_shen@merck.com
Yao
Zuliang
Z
Johnson
Tracy
T
Feeney
Williams P
WP
Jennings
Veronica
V
Xu
Suoyu S
SS
Liesch
Jerrold
J
Tong
Xinchun
X
D000284:Administration, Oral; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002406:Catheterization, Peripheral / Q000379:methods / Q000662:veterinary*; D002408:Catheters, Indwelling / Q000662:veterinary; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D007275:Injections, Intravenous; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011169:Portal Vein / Q000601:surgery*; D020127:Recovery of Function / Q000187:drug effects; D015262:Xenobiotics / Q000008:administration & dosage* / Q000493:pharmacokinetics
D015262:Xenobiotics