title: NS5ATP9, a gene up-regulated by HCV NS5A protein.
abstract: Non-structural protein 5A (NS5A) appears to interact with a variety of cellular proteins and play an important role in mediating cell growth, cellular signaling pathways and pathogenesis of hepatitis C virus (HCV). NS5ATP9 was identified as a NS5A trans-activated protein in suppression subtractive hybridization (SSH), and the regulation was confirmed by luciferase reporter assay and quantitative real time PCR (qRT-PCR). A minimal promoter region contained within the 211bp (nucleotides -161 to +50bp) immediately upstream of the transcription initiation site. NS5ATP9 is a NS5A up-regulation gene which may play a role in the pathogenesis of HCV-associated hepatocellular carcinoma.
authors: The First Hospital of Xi'an Jiaotong University, Xi'an, China.
authors: Lei
authors: L
authors: Shi
authors: Shu-Lin
authors: SL
authors: Zhang
authors: Kang
authors: K
authors: Li
authors: Yuan
authors: Y
authors: Hong
authors: Qi
authors: Q
authors: Wang
authors: Yue
authors: Y
authors: Li
authors: Jiang
authors: J
authors: Guo
authors: Wan-hu
authors: WH
authors: Fan
authors: Lei
authors: L
authors: Zhang
authors: Jun
authors: J
authors: Cheng
mesh_terms: D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D017930:Genes, Reporter; D016174:Hepacivirus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D006526:Hepatitis C / Q000150:complications* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D015533:Transcriptional Activation; D014162:Transfection; D015854:Up-Regulation; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D017361:Viral Nonstructural Proteins; C425208:PCLAF protein, human; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase