The stem cell marker prominin-1/CD133 on membrane particles in human cerebrospinal fluid offers novel approaches for studying central nervous system disease.
Cerebrospinal fluid (CSF) is routinely used for diagnosing and monitoring neurological diseases. The CSF proteins used so far for diagnostic purposes (except for those associated with whole cells) are soluble. Here, we show that human CSF contains specific membrane particles that carry prominin-1/CD133, a neural stem cell marker implicated in brain tumors, notably glioblastoma. Differential and equilibrium centrifugation and detergent solubility analyses showed that these membrane particles were similar in physical properties and microdomain organization to small membrane vesicles previously shown to be released from neural stem cells in the mouse embryo. The levels of membrane particle-associated prominin-1/CD133 declined during childhood and remained constant thereafter, with a remarkably narrow range in healthy adults. Glioblastoma patients showed elevated levels of membrane particle-associated prominin-1/CD133, which decreased dramatically in the final stage of the disease. Hence, analysis of CSF for membrane particles carrying the somatic stem cell marker prominin-1/CD133 offers a novel approach for studying human central nervous system disease.
Huttner
Hagen B
HB
Tissue Engineering Laboratories, Biotechnology Center (BIOTEC), Technische Universität Dresden, Tatzberg 47-51, 01307 Dresden, Germany. hagen.huttner@uk-erlangen.de
Janich
Peggy
P
Köhrmann
Martin
M
Jászai
József
J
Siebzehnrubl
Florian
F
Blümcke
Ingmar
I
Suttorp
Meinolf
M
Gahr
Manfred
M
Kuhnt
Daniela
D
Nimsky
Christopher
C
Krex
Dietmar
D
Schackert
Gabriele
G
Löwenbrück
Kai
K
Reichmann
Heinz
H
Jüttler
Eric
E
Hacke
Werner
W
Schellinger
Peter D
PD
Schwab
Stefan
S
Wilsch-Bräuninger
Michaela
M
Marzesco
Anne-Marie
AM
Corbeil
Denis
D
D000071916:AC133 Antigen; D015703:Antigens, CD / Q000134:cerebrospinal fluid*; D015415:Biomarkers / Q000378:metabolism; D018938:Caco-2 Cells; D002493:Central Nervous System Diseases / Q000134:cerebrospinal fluid*; D005909:Glioblastoma / Q000134:cerebrospinal fluid; D006023:Glycoproteins / Q000134:cerebrospinal fluid*; D006801:Humans; D010455:Peptides / Q000134:cerebrospinal fluid*; D012015:Reference Standards; D013234:Stem Cells / Q000378:metabolism*; D013997:Time Factors
D000071916:AC133 Antigen; D015703:Antigens, CD; D015415:Biomarkers; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse