Nanocrystalline diamond: In vitro biocompatibility assessment by MG63 and human bone marrow cells cultures.
Nanocrystalline diamond (NCD) has a great potential for prosthetic implants coating. Nevertheless, its biocompatibility still has to be better understood. To do so, we employed several materials characterization techniques (SEM, AFM, micro-Raman spectroscopy) and cell culture assays using MG63 osteoblast-like and human bone marrow cells. Biochemical routines (MTT assays, Lowry's method, ALP activity) supported by SEM and confocal microscopy characterization were carried out. We used silicon nitride (Si3N4) substrates for NCD coatings based on a previous demonstration of the superior adhesion and tribological performance of these NCD coated ceramics. Results demonstrate an improved human osteoblast proliferation and the stimulation of differentiated markers, like ALP activity and matrix mineralization, compared with standard polystyrene tissue culture plates. The nanometric featuring of NCD, associated to its chemical affinity are key points for bone regeneration purposes.
Amaral
M
M
CICECO, Department of Ceramics and Glass Engineering, University of Aveiro, 3810-193 Aveiro, Portugal.
Dias
A G
AG
Gomes
P S
PS
Lopes
M A
MA
Silva
R F
RF
Santos
J D
JD
Fernandes
M H
MH
D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D018130:Diamond / Q000737:chemistry*; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D008297:Male; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D019736:Prostheses and Implants; D017655:Silicon Compounds / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D023822:Tissue Engineering
D020099:Coated Materials, Biocompatible; D017655:Silicon Compounds; D018130:Diamond; D000469:Alkaline Phosphatase; C032734:silicon nitride