H5-type influenza virus hemagglutinin is functionally recognized by the natural killer-activating receptor NKp44.
Antiviral immune defenses involve natural killer (NK) cells. We previously showed that the NK-activating receptor NKp44 is involved in the functional recognition of H1-type influenza virus strains by NK cells. In the present study, we investigated the interaction of NKp44 and the hemagglutinin of a primary influenza virus H5N1 isolate. Here we show that recombinant NKp44 recognizes H5-expressing cells and specifically interacts with soluble H5 hemagglutinin. H5-pseudotyped lentiviral particles bind to NK cells expressing NKp44. Following interaction with target cells expressing H5, pseudotyped lentiviral particles, or membrane-associated H5, NK cells show NKp44-mediated induced activity. These findings indicate that NKp44-H5 interactions induce functional NK activation.
Ho
Joanna W
JW
Shraga Segal Department of Microbiology and Immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
Hershkovitz
Oren
O
Peiris
Malik
M
Zilka
Alon
A
Bar-Ilan
Ahuva
A
Nal
Beatrice
B
Chu
Kid
K
Kudelko
Mateusz
M
Kam
Yiu Wing
YW
Achdout
Hagit
H
Mandelboim
Michal
M
Altmeyer
Ralf
R
Mandelboim
Ofer
O
Bruzzone
Roberto
R
Porgador
Angel
A
D002460:Cell Line; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology* / Q000821:virology; D055629:Natural Cytotoxicity Triggering Receptor 2; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D019267:Hemagglutinin Glycoproteins, Influenza Virus; C112837:NCR2 protein, human; D055629:Natural Cytotoxicity Triggering Receptor 2; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; C109963:hemagglutinin, avian influenza A virus