[The genes associated with termination of the critical period of neuroplasticity in visual cortex].
OBJECTIVE
To screening genes associated with termination of the critical period of neuroplasticity in visual cortex and investigate the molecular mechanism.


METHODS
To construct a cDNA library using visual cortex of dark rearing rats and normal light rearing 1 week after dark rearing 60 days rats with suppression subtractive hybridization technique. PCR, reverse Northern hybridization, sequencing and homology search were used to analysis the differential expression genes fragments.


RESULTS
The cDNA library of termination the critical period was set up successfully. After screening, sequencing and homology search 14 sequences were obtained. Among of the genes 13 were known genes and one was novel gene fragment. They were up-regulated genes in visual cortex when the critical period termination.


CONCLUSIONS
Through construction the cDNA library, we screened a crop of candidate genes related to termination the critical period. The results provided important foundation to further illuminate the molecular mechanism of termination the critical period of neuroplasticity in visual cortex.
Jiang
Wen-shan
WS
The Southwest Ophthalmology Hospital of the Third Military Medical University, Chongqing 400038, China.
Yin
Zheng-qin
ZQ
D000818:Animals; D015723:Gene Library; D005820:Genetic Testing; D009473:Neuronal Plasticity / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D014793:Visual Cortex