Could cyclophosphamide exert a protective role avoiding esophagic neuron loss in Calomys callosus infected with Trypanosoma cruzi?
The protective role of cyclophosphamide was studied in this work. Young male Calomys callosus were infected with Trypanosoma cruzi and allowed to age. Cyclophosphamide therapy was administered to animals during acute and late chronic phases of infection. Esophageal neurons were counted, displaying enhanced neuronal loss for the young and treated infected groups. For aged and cyclophosphamide treated animals, a protection was observed through a reduced loss of neurons as compared to the young and infected groups. Enhanced nitric oxide concentrations were observed for young animals as compared to aged counterparts. Splenocyte proliferation was reduced during the acute phase in comparison with those found in the chronic phase. Morphometry of neuronal body displayed a significant reduction concerning the area, perimeter, diameter and volume for aged animals as compared to young groups. These results indicate that the protective effects of cyclophosphamide together with process of neuroplasty of peripheral nervous system could lead to a protection against neuronal loss.
Caetano
Leony Cristina
LC
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (FCFRP-USP), Ribeirao Preto, SP, Brazil. leony@fcfrp.usp.br
Zucoloto
Sérgio
S
Kawasse
Laura Midori
LM
Alonsotoldo
Miriam Paula
MP
do Prado
José Clóvis
JC
D000367:Age Factors; D000818:Animals; D003411:Arvicolinae; D016923:Cell Death; D049109:Cell Proliferation / Q000187:drug effects; D014355:Chagas Disease / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003520:Cyclophosphamide / Q000494:pharmacology*; D004195:Disease Models, Animal; D004947:Esophagus / Q000294:innervation*; D008297:Male; D009197:Myenteric Plexus / Q000187:drug effects* / Q000469:parasitology / Q000473:pathology; D009474:Neurons / Q000187:drug effects* / Q000469:parasitology / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D013154:Spleen / Q000187:drug effects / Q000469:parasitology; D014349:Trypanosoma cruzi
D018696:Neuroprotective Agents; D009569:Nitric Oxide; D003520:Cyclophosphamide