Salinity-dependent changes in Na(+)/K (+)-ATPase content of mitochondria-rich cells contribute to differences in thermal tolerance of Mozambique tilapia.
The Mozambique tilapia (Oreochromis mossambicus) is prone to osmoregulatory disturbances when faced with fluctuating ambient temperatures. To investigate the underlying causes of this phenomenon, freshwater (FW)- and seawater (SW)-acclimated tilapia were transferred to 15, 25, or 35 degrees C for 2 weeks, and along with typically used indicators of osmoregulatory status [plasma osmolality and branchial and intestinal specific Na(+), K(+)-ATPase (NKA) activity], we used tissue microarrays (TMA) and laser-scanning cytometry (LSC) to characterize the effects of temperature acclimation. Tissue microarrays were stained with fluorescently labeled anti-Na(+), K(+)-ATPase antibodies that allowed for the quantification of NKA abundance per unit area within individual branchial mitochondria-rich cells (MRCs) as well as sections of renal tissue. Mitochondria-rich cell counts and estimates of size were carried out for each treatment by the detection of DASPMI fluorescence. The combined analyses showed that SW fish have larger but fewer MRCs that contain more NKA per unit area. After a 2-week acclimation to 15 degrees C tilapia experienced osmotic imbalances in both FW and SW that were likely due to low NKA activity. SW-acclimated fish compensated for the low activity by increasing MRC size and subsequently the concentration of NKA within MRCs. Although there were no signs of osmotic stress in FW-acclimated tilapia at 25 degrees C, there was an increased NKA capacity that was most likely mediated by a higher MRC count. We conclude on the basis of the different responses to temperature acclimation that salinity-induced changes in the NKA concentration of MRCs alter thermal tolerance limits of tilapia.
Sardella
Brian A
BA
Department of Zoology, University of British Columbia, 6270 University Blvd, Vancouver, BC, Canada, V6T 1Z4. basardella@ucdavis.edu
Kültz
Dietmar
D
Cech
Joseph J
JJ
Brauner
Colin J
CJ
D000064:Acclimatization / Q000502:physiology; D000818:Animals; D001833:Body Temperature Regulation / Q000502:physiology*; D005880:Gills / Q000166:cytology / Q000201:enzymology; D007422:Intestines / Q000166:cytology / Q000201:enzymology; D007668:Kidney / Q000166:cytology / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology*; D054712:Salinity; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013696:Temperature; D017210:Tilapia / Q000502:physiology*; D014882:Water-Electrolyte Balance / Q000502:physiology
D000254:Sodium-Potassium-Exchanging ATPase