Synthesis of modified DNA by PCR with alkyne-bearing purines followed by a click reaction.
Alkyne-bearing deazapurine triphosphates were prepared and successfully incorporated into DNA using the polymerase chain reaction (PCR). The obtained alkyne-labeled DNA was successfully used in a click reaction with galactose azide.
Gramlich
Philipp M E
PM
Department of Chemistry, Ludwig-Maximilians University Munich, Butenandtstr. 13, D-81377 Munich, Germany.
Wirges
Christian T
CT
Gierlich
Johannes
J
Carell
Thomas
T
D001386:Azides / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005690:Galactose / Q000031:analogs & derivatives / Q000737:chemistry; D015394:Molecular Structure; D016133:Polymerase Chain Reaction; D011685:Purine Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry
D001386:Azides; D011685:Purine Nucleotides; D004247:DNA; D005690:Galactose