title: Identification of Vibrio spp. in vibriosis Penaeus vannamei using developed monoclonal antibodies.
abstract: Immunohistochemical study using monoclonal antibodies specific to various shrimp viruses and Vibrio spp. was performed in shrimp samples died from unknown cause with symptoms of black stripes on lateral sides of cephalothorax or smoky body coloration. The positive results in muscular tissue were obtained with MAb VAL57 (specific to Vibrio spp.) and in hepatopancreas tissues with MAbs VVB158 (specific to V. vulnificus) and VPC701 (specific to V. parahaemolyticus). Twelve isolates of Vibrio spp. isolated from shrimp tissues were identified with various MAbs by dot blotting, biochemical tests and 16S rRNA gene. The results revealed three groups of V. vulnificus and one group of V. shilonii. All four groups of isolated Vibrio spp. were immunologically and biochemically different. None of the V. parahaemolyticus-like bacterium was isolated. The results demonstrated that the mortality in shrimp is accompanied by the presence of Vibrio spp.
authors: Department of Biology, Faculty of Science, Srinakharinwirot University, Sukhumvit 23, Bangkok 10110, Thailand.
authors: Siwaporn
authors: S
authors: Longyant
authors: Sombat
authors: S
authors: Rukpratanporn
authors: Parin
authors: P
authors: Chaivisuthangkura
authors: Pornthip
authors: P
authors: Suksawad
authors: Chutima
authors: C
authors: Srisuk
authors: Weerawan
authors: W
authors: Sithigorngul
authors: Somkiat
authors: S
authors: Piyatiratitivorakul
authors: Paisarn
authors: P
authors: Sithigorngul
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000276:immunology; D017756:Aquaculture; D005798:Genes, Bacterial / Q000235:genetics; D020459:Genes, rRNA / Q000235:genetics; D033561:Penaeidae / Q000382:microbiology*; D014733:Vibrio / Q000145:classification* / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial