title: Regeneration of dentin-pulp complex with cementum and periodontal ligament formation using dental bud cells in gelatin-chondroitin-hyaluronan tri-copolymer scaffold in swine.
abstract: The purpose of this study is to use a tissue engineering approach for tooth regeneration. The swine dental bud cells (DBCs) were isolated from the developing mandibular teeth, expanded in vitro, and cultured onto cylinder scaffold gelatin-chrondroitin-hyaluronan-tri-copolymer (GCHT). After culturing in vitro, the DBCs/GCHT scaffold was autografted back into the original alveolar socket. Hematoxylin and eosin (H&E) staining combined with immunohistochemical staining were applied for identification of regenerated tooth structure. After 36-week post-transplantation, tooth-like structures, including well-organized dentin-pulp complex, cementum, and periodontal ligament, were evident in situ in two of six experimental animals. The size of the tooth structure (1 x 0.5 x 0.5 cm(3) and 0.5 x 0.5 x 0.5 cm(3) size) appeared to be dictated by the size of the GCHT scaffold (1 x 1 x 1.5 cm(3)). The third swine was demonstrated with irregular dentin-bony like calcified tissue about 1 cm in diameter without organized tooth or periodontal ligament formation. The other three swine in the experimental group showed normal bone formation and no tooth regeneration in the transplantation sites. The successful rate of tooth regeneration from DBCs/GCHT scaffolds' was about 33.3%. In the control group, three swine's molar teeth buds were removed without DBCs/GCHT implantation, the other three swine received GCHT scaffold implants without DBCs. After evaluation, no regenerated tooth was found in the transplantation site of the control group. The current results using DBSs/GCHT scaffold autotransplantation suggest a technical breakthrough for tooth regeneration.
authors: Graduate Institute of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
authors: Tzong-Fu
authors: TF
authors: Kuo
authors: An-Ting
authors: AT
authors: Huang
authors: Hao-Hueng
authors: HH
authors: Chang
authors: Feng-Huei
authors: FH
authors: Lin
authors: San-Tai
authors: ST
authors: Chen
authors: Rung-Shu
authors: RS
authors: Chen
authors: Cheng-Hung
authors: CH
authors: Chou
authors: Hsin-Chi
authors: HC
authors: Lin
authors: Han
authors: H
authors: Chiang
authors: Min-Huey
authors: MH
authors: Chen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002807:Chondroitin / Q000494:pharmacology / Q000648:ultrastructure; D003739:Dental Cementum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D003804:Dentin / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D005780:Gelatin / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000494:pharmacology / Q000648:ultrastructure; D007150:Immunohistochemistry; D007568:Jaw / Q000000981:diagnostic imaging; D056948:Orthognathic Surgical Procedures; D010513:Periodontal Ligament / Q000187:drug effects / Q000378:metabolism*; D011859:Radiography; D012038:Regeneration / Q000187:drug effects*; D013194:Staining and Labeling; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014070:Tooth / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D005780:Gelatin; D006820:Hyaluronic Acid; D002807:Chondroitin