Novel rice OsSIPK is a multiple stress responsive MAPK family member showing rhythmic expression at mRNA level.
We report isolation and transcriptional profiling of rice (Oryza sativa L.) mitogen-activated protein kinase (MAPK), OsSIPK (salicylic acid-induced protein kinase). OsSIPK gene is located on chromosome 6 most probably existing as a single copy in the rice genome, and encodes 398 amino acid polypeptide having the MAPK family signature and phosphorylation activation motif TEY. Steady state mRNA analyses of OsSIPK showed weak constitutive expression in leaves of 2-week-old rice seedlings. A time course (30-120 min) experiment using a variety of elicitors and stresses revealed that the OsSIPK mRNA is strongly induced by jasmonic acid (JA), salicylic acid (SA), ethephon, abscisic acid, cycloheximide (CHX), JA/SA + CHX, cantharidin, okadaic acid, hydrogen peroxide, chitosan, sodium chloride, and cold stress (12 degrees C), but not with wounding by cut, gaseous pollutants ozone, and sulfur dioxide, high temperature, ultraviolet C irradiation, sucrose, and drought. Its transcription was also found to be tissue-specifically regulated, and followed a rhythmic dark induction in leaves. Finally, we showed that the OsSIPK protein is localized to the nucleus. From these results, OsSIPK can be implicated in diverse stimuli-responsive signaling cascades and transcription of certain genes.
Lee
Mi-Ok
MO
Department of Molecular Biology, College of Natural Science, Sejong University, Gwangjin-Gu, Seoul, South Korea.
Cho
Kyoungwon
K
Kim
So-Hee
SH
Jeong
Seung-Hee
SH
Kim
Jung-A
JA
Jung
Young-Ho
YH
Shim
Jaekyung
J
Shibato
Junko
J
Rakwal
Randeep
R
Tamogami
Shigeru
S
Kubo
Akihiro
A
Agrawal
Ganesh Kumar
GK
Jwa
Nam-Soo
NS
D000040:Abscisic Acid / Q000494:pharmacology; D015152:Blotting, Northern; D002193:Cantharidin / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D048271:Chitosan / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics*; D019319:Okadaic Acid / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000494:pharmacology; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D054883:Oxylipins / Q000494:pharmacology; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D020156:Salicylic Acid / Q000494:pharmacology; D036226:Seedlings / Q000201:enzymology / Q000235:genetics; D017422:Sequence Analysis, DNA; D013458:Sulfur Dioxide / Q000494:pharmacology; D014466:Ultraviolet Rays
D003517:Cyclopentanes; D009943:Organophosphorus Compounds; D054883:Oxylipins; D010940:Plant Proteins; D012333:RNA, Messenger; D013458:Sulfur Dioxide; D019319:Okadaic Acid; C011006:jasmonic acid; D000040:Abscisic Acid; D048271:Chitosan; D003513:Cycloheximide; D006861:Hydrogen Peroxide; D020928:Mitogen-Activated Protein Kinases; C106405:SA-induced protein kinase; D002193:Cantharidin; D020156:Salicylic Acid; C005073:ethephon