Genetic variability in E6 and E7 genes of human papillomavirus -16, -18, -31 and -33 from HIV-1-positive women in Italy.
Among 544 HIV-positive women screened for HPV-DNA between 2003 and 2005, 265 (48.7%) were HPV-positive: 24 (9.1%) harboured HPV-16, 21 (7.9%) HPV-31, 12 (4.5%) HPV-18, 7 (2.6%) HPV-33. E6 and E7 of these HPV types were sequenced to assess their diversity. Ranges of inter- and intra-variant diversity were 1.2-3-3%, and 0.8-1.8 for E6 and 0.6-2.7%, and 0.6-2.0% for E7, respectively. HPV-31, the second most common HPV type, showed the highest diversity for both regions. On the whole, 26 out of 59 mutations were non-synonymous. The variability of these proteins may have implications in HPV vaccine strategies.
Garbuglia
Anna Rosa
AR
Laboratory of Virology, Lazzaro Spallanzani National Institute of Infectious Diseases INMI, Rome, Italy.
Carletti
Fabrizio
F
Minosse
Claudia
C
Piselli
Pierluca
P
Zaniratti
M Stefania
MS
Serraino
Diego
D
Capobianchi
M Rosaria
MR
D000328:Adult; D002584:Cervix Uteri / Q000821:virology; D005260:Female; D005814:Genes, Viral; D014644:Genetic Variation; D015658:HIV Infections / Q000150:complications*; D015497:HIV-1; D006801:Humans; D007558:Italy; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D050725:Papillomavirus E7 Proteins / Q000235:genetics; D030361:Papillomavirus Infections / Q000150:complications* / Q000821:virology*
D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins