title: Antibody to the Chlamydia trachomatis 60 kDa heat shock protein in follicular fluid and in vitro fertilization outcome.
abstract: OBJECTIVE
  The association between 60 kDa Chlamydia trachomatis heat shock protein (CHSP60) antibodies and the etiology and outcomes of in vitro fertilization (IVF) outcomes is not well known.
  
  
  METHODS
  A retrospective study with a double-blind analysis of follicular fluid from 253 IVF patients for IgG antibodies to CHSP60.
  
  
  RESULTS
  The CHSP60 antibodies were detected in 74.1% of women without embryo implantation and in 47.9% of women with 1-3 implantations per IVF cycle (P = 0.0004). CHSP60 antibodies were detected in 69.5% of women with tubal occlusion and 49.7% of women with other causes of infertility (P = 0.01). CHSP60 antibody detection was unrelated to maternal age, number of oocytes collected, or percentage of oocytes fertilized.
  
  
  CONCLUSIONS
  Detection of IgG antibody to CHSP60 may indicate persistence of C. trachomatis in the upper genital tract with low implantation rates resulting from a chronic inflammatory reaction. Alternatively, as human hsp60 is expressed in early stage embryogenesis, a cross-reacting antibody may induce destruction of the embryo.
authors: Division of Immunology and Infectious Diseases, Department of Obstetrics and Gynecology, Weill Medical College of Cornell University, New York, NY, USA. sharonjakus@hotmail.com
authors: Sharon
authors: S
authors: Jakus
authors: Andreas
authors: A
authors: Neuer
authors: Stefan
authors: S
authors: Dieterle
authors: Ann Marie
authors: AM
authors: Bongiovanni
authors: Steven S
authors: SS
authors: Witkin
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D016022:Case-Control Studies; D002690:Chlamydia Infections / Q000276:immunology* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000276:immunology*; D004311:Double-Blind Method; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007247:Infertility, Female / Q000276:immunology / Q000382:microbiology*; D008297:Male; D011247:Pregnancy; D012189:Retrospective Studies; D016896:Treatment Outcome
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G; C067562:protein 60K, Chlamydia trachomatis