Immunohistochemical staining of plastic (methyl-methacrylate)-embedded bone marrow biopsies applying the biotin-free tyramide signal amplification system.
Immunohistochemistry of trephine bone marrow biopsies plays a prominent role in diagnostic assessment of a broad spectrum of non-neoplastic and neoplastic hematologic and metastatic diseases. We tested the quality of immunohistochemical stainings in sections of methyl-methacrylate-embedded core biopsies prepared according to the so-called Hannover-method of cold polymerization using the novel biotin-free tyramide signal amplification system in comparison to the alkaline phosphatase-conjugated "EnVision" method. Examining a large panel of immunohistochemical markers, the amplification system proved to be a highly sensitive technique substantially superior to the EnVision-AP method in reliably detecting the antigenic determinants of the target cells and referred to the intensity of immunoprecipitations.
Kunze
Ekkehard
E
Department of Osteopathology and Haematopathology, University of Goettingen, D-37099 Goettingen, Germany. ekunze@med.uni-goettingen.de
Middel
Peter
P
Fayyazi
Afshin
A
Schweyer
Stefan
S
D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D001853:Bone Marrow / Q000737:chemistry* / Q000276:immunology / Q000473:pathology; D016591:Histocytological Preparation Techniques / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007938:Leukemia / Q000175:diagnosis; D008223:Lymphoma / Q000175:diagnosis; D020366:Methylmethacrylate; D016613:Plastic Embedding; D013601:T-Lymphocytes / Q000276:immunology; D016707:Tissue Fixation / Q000379:methods
D015703:Antigens, CD; D020366:Methylmethacrylate