Bioactive microspheres produced from gelatin-siloxane hybrids for bone regeneration.
In this study, we produced a novel microsphere with a hybridized composition of gelatin and siloxane which is bioactive and degradable for the applications in bone regeneration fields. A solution of gelatin organic and siloxane inorganic containing calcium chloride was formulated into microspheres in an oil bath mediated by a surfactant. Following the processes of hydration, gelation and solidification, well-shaped spherical particles were produced with sizes of a few to hundreds of micrometers (68 microm on average). The obtained microspheres were highly stable in an aqueous solution due to the in situ cross-linking of the siloxane with gelatin chains, which eliminated the additional cross-linking step generally used in pure gelatin. The hybridized microspheres exhibited rapid induction of apatite-like crystals on their surface with incubation in a simulated body fluid, suggesting an excellent bone bioactivity in vitro. It is considered that the newly developed organic-inorganic microspheres may be useful for the regeneration of skeletal defects.
Yoon
Byung-Ho
BH
School of Materials Science and Engineering, Seoul National University, Seoul, 151-742, Korea.
Kim
Hyoun-Ee
HE
Kim
Hae-Won
HW
D000818:Animals; D001031:Apatites / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D001826:Body Fluids / Q000737:chemistry; D001861:Bone Regeneration; D001842:Bone and Bones / Q000378:metabolism; D002417:Cattle; D005780:Gelatin / Q000737:chemistry*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D012833:Siloxanes / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013501:Surface-Active Agents / Q000737:chemistry; D014867:Water / Q000737:chemistry
D001031:Apatites; D001672:Biocompatible Materials; D012833:Siloxanes; D013501:Surface-Active Agents; D014867:Water; D005780:Gelatin