Hydrogen-deuterium exchange and selective labeling of deprotonated amino acids and peptides in the gas phase.
Hydrogen-deuterium exchange reactions of deprotonated amino acids and small peptides were studied. Selective labeling can be carried out at the alpha-amino group of lysine (2 of 4 labile hydrogens undergo exchange with CF3CH2OD) and the guanidine side chain of arginine (3 of 6 labile hydrogens undergo exchange with CH3CH2OD and C6H5CH2OD). Differential labeling of peptides also was accomplished, and the extent of H/D exchange is dependent on the amino acids which are present as well as their order in the chain.
Tian
Zhixin
Z
Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Lis
Lev
L
Kass
Steven R
SR
D000596:Amino Acids / Q000737:chemistry*; D041961:Deuterium Exchange Measurement; D005740:Gases / Q000737:chemistry; D007553:Isotope Labeling / Q000379:methods*; D010455:Peptides / Q000737:chemistry*
D000596:Amino Acids; D005740:Gases; D010455:Peptides