Live cells-based cytotoxic sensorchip fabricated in a microfluidic system.
We report here a live cells-based sensorchip fabricated in microfluidic channels in which several laminar flows were achieved. In addition, we established a cytotoxic sensor cell line, which was transfected with a green fluorescence protein (GFP) plasmid derived from the heat shock protein 70B' (HSP70B') promoter, which is induced by cytotoxic reagents. The fluorescence in the sensor cells increased in a CdCl(2) dose-dependent manner in the microfluidic channels. In this system, cytotoxic reagents can be quantitatively detected in a quick, sensitive and high-throughput manner. The combination of sensor cells and microfluidic systems will provide an important basis for the development of micro-total analysis systems (micro-TAS) technology, and can be applied to toxicology, environmental assessment and drug screening.
Wada
Ken-Ichi
K
Cell-Sensing Group, Biomaterials Center, National Institute for Materials Science, 1-1, Namiki, Tsukuba, Ibaraki 305-0044, Japan.
Taniguchi
Akiyoshi
A
Kobayashi
Jun
J
Yamato
Masayuki
M
Okano
Teruo
T
D000818:Animals; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D041681:NIH 3T3 Cells; D018675:Toxicity Tests / Q000295:instrumentation* / Q000379:methods