[Effects of mouse bone marrow mesenchymal stem cells on the expression of chemokine receptors of spleenocytes after phytohemagglutinin stimulation].
OBJECTIVE
To explore the effect of mouse bone marrow mesenchymal stem cells (MSCs) on the expression of chemokine receptors in T lymphocytes in vitro.


METHODS
Mouse bone marrow MSCs were separated with Percoll, cultured and expanded in low glucose DMEM. C57BL/6 mouse spleenocytes were cultured in the 24-hole flasks by the density of 1 x10(6)/hole. Phytohemagglutinin (PHA) was then added to the holes and cultured for 72 hrs. This study consisted of three groups. Groups A and B were co-cultured by adding MSCs as the ratio of 0.1 and 0.01 to spleenocytes respectively. The control group was cultured without MSCs. Three days later the suspended spleenocytes were harvested for detecting the expression of three chemokine receptors CXCR3, CCR5 and CCR7 in T lymphocytes by the flow cytometry.


RESULTS
The expression of CD3(+)CCR5(+) and CD3(+)CCR7(+) were statistically different among the three groups. Group A had the strongest expression, followed by group B and the control group. The expression of CD3(+)CXCR3(+) in group A was statistically higher than that in group B and the control group.


CONCLUSIONS
MSCs could up-regulate the expression of chemokine receptors CXCR3, CCR5 and CCR7 in T lymphocytes stimulated by PHA.
Liu
Yuan-Chun
YC
Department of Pediatrics, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Feng
Xiao-Qin
XQ
He
Yue-Lin
YL
Wu
Xue-Dong
XD
Zhang
Yu-Ming
YM
Li
Chun-Fu
CF
D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D002478:Cells, Cultured; D008213:Lymphocyte Activation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010835:Phytohemagglutinins / Q000494:pharmacology*; D019713:Receptors, CCR5 / Q000032:analysis*; D054400:Receptors, CCR7 / Q000032:analysis*; D054367:Receptors, CXCR3 / Q000032:analysis*; D013154:Spleen / Q000166:cytology* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
C515371:Ccr7 protein, mouse; C515188:Cxcr3 protein, mouse; D010835:Phytohemagglutinins; D019713:Receptors, CCR5; D054400:Receptors, CCR7; D054367:Receptors, CXCR3