I kappa B alpha promoter polymorphisms in patients with systemic lupus erythematosus.
To investigate the associations of IkappaBalpha gene polymorphisms with the development and clinical manifestations of systemic lupus erythematosus (SLE), 110 patients with SLE and 120 unrelated healthy controls were enrolled in this study. The IkappaBalpha -881 A/G, -826 C/T, -550 A/T, -519 C/T, and -297 C/T polymorphisms were determined by the polymerase chain reaction/reaction fragment length polymorphism method. The genotype frequency of IkappaBalpha -826 C/T in the patients with SLE was significantly higher than that of the controls (p = 0.003, OR = 2.2, 95% CI = 1.3-3.9). The SLE patients also have significantly higher carriage rate of IkappaBalpha -826 T than the controls (p = 0.01, OR = 2.0, 95% CI = 1.2-3.4). We also found that the estimated haplotype frequency of IkappaBalpha -881A -826T -550A -519C -297C was significantly increased in the patients with SLE in comparison with that of the controls. This study also demonstrated that the association of IkappaBalpha -826 T with SLE was independent of HLA-DR15, which is associated with susceptibility to SLE in Taiwan. Moreover, a synergistic effect could also be found between IkappaBalpha -826 T and HLA-DR15. IkappaBalpha -826 T is associated with the development of SLE in Taiwan. The IkappaBalpha -881A -826T -550A -519C -297C haplotype is also associated with susceptibility to SLE. This study also demonstrated that IkappaBalpha -881G was associated with the occurrence of vasculitis in SLE patients. IkappaBalpha -550T might be a protective factor for the development of malar rash.
Lin
Chia-Hui
CH
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Zihyou 1st Road, Kaohsiung City 807, Taiwan.
Wang
Shu-Chen
SC
Ou
Tsan-Teng
TT
Li
Ruei-Nian
RN
Tsai
Wen-Chan
WC
Liu
Hong-Wen
HW
Yen
Jeng-Hsien
JH
D000328:Adult; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D020672:I-kappa B Proteins / Q000235:genetics*; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D008297:Male; D000072000:NF-KappaB Inhibitor alpha; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013624:Taiwan; D013936:Thymidine / Q000235:genetics
D020672:I-kappa B Proteins; C000606204:NFKBIA protein, human; D000072000:NF-KappaB Inhibitor alpha; D013936:Thymidine