[Rapid testing on drug susceptibility of Mycobacterium tuberculosis with nitrate reducrase assay].
OBJECTIVE
To establish a rapid method for testing drug sussceptibility on Mycobacterium tuberculosis.


METHODS
Taking absolute Concentration method for drug susceptibility testing of M. tuberculosis as the "gold standard", we examined the drug-resistant of M. tuberculosis strain with nitrate reducrase assay (NRA) and the drug-resistant of M. tuberculosis germ in sputum with NRA.


RESULTS
NRA and absolute concentration method was basically comparable with NRA susceptibility as 96.5% and the specificity was 100%, When comparing with traditional absolute concentration method, NRA could shorten the time about 3 weeks. Using NRA to test the drug-resistant of M. tuberculosis germ in sputum, its susceptibility was more than 66.7% and specificity was 100%, within 10-20 days.


CONCLUSIONS
NRA could be used as a rapid drug susceptibility testing on M. tuberculosis.
Liu
Zhong-Quan
ZQ
Department of Bacteriology and Immunology, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China.
Li
Chuan-You
CY
Chen
Xiao-You
XY
Chen
Xi
X
Ma
Yu
Y
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D057075:Enzyme Assays / Q000379:methods*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000472:pathogenicity; D050901:Nitrate Reductase / Q000378:metabolism*; D013183:Sputum / Q000382:microbiology
D000900:Anti-Bacterial Agents; D050901:Nitrate Reductase