Development of amphiphilic gamma-PGA-nanoparticle based tumor vaccine: potential of the nanoparticulate cytosolic protein delivery carrier.
Nanoscopic therapeutic systems that incorporate biomacromolecules, such as protein and peptides, are emerging as the next generation of nanomedicine aimed at improving the therapeutic efficacy of biomacromolecular drugs. In this study, we report that poly(gamma-glutamic acid)-based nanoparticles (gamma-PGA NPs) are excellent protein delivery carriers for tumor vaccines that delivered antigenic proteins to antigen-presenting cells and elicited potent immune responses. Importantly, gamma-PGA NPs efficiently delivered entrapped antigenic proteins through cytosolic translocation from the endosomes, which is a key process of gamma-PGA NP-mediated anti-tumor immune responses. Our findings suggest that the gamma-PGA NP system is suitable for the intracellular delivery of protein-based drugs as well as tumor vaccines.
Yoshikawa
Tomoaki
T
Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Okada
Naoki
N
Oda
Atsushi
A
Matsuo
Kazuhiko
K
Matsuo
Keisuke
K
Mukai
Yohei
Y
Yoshioka
Yasuo
Y
Akagi
Takami
T
Akashi
Mitsuru
M
Nakagawa
Shinsaku
S
D000818:Animals; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology*; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology; D004337:Drug Carriers / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011099:Polyglutamic Acid / Q000737:chemistry*; D011506:Proteins / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology*
D019496:Cancer Vaccines; D004337:Drug Carriers; D011506:Proteins; D011099:Polyglutamic Acid