[An epidemiological study of rabies virus in domestic dogs, cats and wildlife and the immunogenicity study for rabies vaccines derived from different cell cultured virus strains].
For epidemiological investigation of the rabies virus carrier rates of domestic dogs, cats and wild animals like rodent animals and bats,three kinds of regions where rabies had higher incidence (Hunan and Guizhou Provinces), lower incidence (Jiangsu Province, Wuhan City) and provisionally rabies-free (Shenyang City) were selected. Then the antigenic types, the genovariation of the isolaled viruses and the currently vaccine matching of the virus strains were analyzed. The results showed that in China the principal host of rabies is dog,the total virus carrier rate of the captured dogs was 2.56%, and the highest positive isolation rate was 20.0% in some monitoring site. However,there was no evidence about the rabies virus carrier rate in rodent animals,bats or other wild animals. The rabies vaccines which prepared from aG and CTN strains have already been produced successfully in China. The research showed that the nucleotide sequences of the newly isolated viruses were more similar with the glycoprotein gene of CTN strain. In order to evaluate the safety and the efficacy of the vaccines currently used, two groups (50 people each) were injected with vaccine of aG strain and CTN strain respectively in five surveillance points. The neutralizing antibody tested were 0.49 IU/mL-0.52 IU/mL and 6.7 IU/mL-7.53 IU/mL after the 7 and the 14 days of vaccine injection respectively. In addition, the rates of antibody positive seroconversion were 45.1%-47.9% and 100% respectively, and there was no moderate or severe adverse reactions observed. These data showed the vaccines have satisfactory effect on safety and protection.
Dong
Guan-Mu
GM
National Institute for the Control of Parmaceutical and Biological Products, Beijing 100050, China. gmdong@nicpbp.org.cn
Xu
Ge-Lin
GL
Xiao
Qi-You
QY
Wang
Ding-Ming
DM
Hu
Yue-Mei
YM
Zhou
Dun-Jin
DJ
Wang
Ping
P
Zhang
Yong-Zhen
YZ
Yang
Xiao-Ming
XM
Zhu
Feng-Cai
FC
Wang
Zhao-Xiao
ZX
Luo
Shu-Bing
SB
Luo
Tong-Yong
TY
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002353:Carrier State / Q000453:epidemiology / Q000662:veterinary*; D002415:Cats / Q000821:virology*; D002522:Chlorocebus aethiops; D004285:Dogs / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014709:Vero Cells
D000914:Antibodies, Viral; D011819:Rabies Vaccines