title: Acute phase response impairs host defense against Pseudomonas aeruginosa pneumonia in mice.
abstract: OBJECTIVE
  Pseudomonas aeruginosa is a common pathogen in hospital-acquired pneumonia. Especially trauma and postsurgical patients display a profound acute phase protein response and are susceptible to acquiring pneumonia. The objective was to study the influence of the acute phase response induced by sterile tissue injury on pulmonary host defense.
  
  
  METHODS
  Laboratory investigation.
  
  
  METHODS
  Academic medical center.
  
  
  METHODS
  Female C57Bl/6 wild-type mice, 8-10 wks old.
  
  
  METHODS
  Mice were injected subcutaneously with either turpentine or sterile saline (control) in both hind limbs 1 day before intranasal infection with P. aeruginosa.
  
  
  RESULTS
  The turpentine-induced acute phase response was associated with 100% lethality after induction of pneumonia, whereas control mice all survived the Pseudomonas infection. In addition, turpentine-injected mice demonstrated much higher bacterial loads in their lungs and an increased dissemination of the infection. The acute phase reaction attenuated lung inflammation during pneumonia, as reflected by histopathology, reduced pulmonary levels of proinflammatory cytokines, and a strongly diminished recruitment of neutrophils to the site of infection. Blood neutrophils harvested from turpentine injected mice displayed a reduced capacity to up-regulate their CD11b/CD18 expression upon stimulation with Pseudomonas ex vivo and during Pseudomonas pneumonia in vivo. Administration of a blocking anti-CD11b antibody to turpentine-injected and control mice almost completely abrogated the difference in bacterial outgrowth, whereas inhibition of the sympathetic nervous system did not affect the impaired pulmonary host defense in mice with an acute phase response.
  
  
  CONCLUSIONS
  These data suggest that a systemic acute phase response might impair host defense against P. aeruginosa pneumonia, possibly in part by inhibition of CD11b/CD18-dependent neutrophil recruitment.
authors: Center for Infection and Immunity Amsterdam, University of Amsterdam, The Netherlands. r.renckens@amc.uva.nl
authors: Rosemarijn
authors: R
authors: Renckens
authors: David J
authors: DJ
authors: van Westerloo
authors: Joris J T H
authors: JJ
authors: Roelofs
authors: Jennie M
authors: JM
authors: Pater
authors: Marcus J
authors: MJ
authors: Schultz
authors: Sandrine
authors: S
authors: Florquin
authors: Tom
authors: T
authors: van der Poll
mesh_terms: D000210:Acute-Phase Reaction / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D018845:CD11 Antigens / Q000502:physiology; D018821:CD18 Antigens / Q000502:physiology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration / Q000502:physiology*; D018410:Pneumonia, Bacterial / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D011552:Pseudomonas Infections / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D011550:Pseudomonas aeruginosa
chemical_list: D018845:CD11 Antigens; D018821:CD18 Antigens; D016207:Cytokines