Potent inhibition of human influenza H5N1 virus by oligonucleotides derived by SELEX.
New therapeutics are urgently needed for the treatment of pandemic influenza caused by H5N1 influenza virus mutants. Aptamer was a promising candidate for treatment and prophylaxis of influenza virus infections. In this study, systemic evolution of ligands through exponential enrichment (SELEX) was used to screen DNA aptamers targeted to recombinant HA1 proteins of the H5N1 influenza virus. After 11 rounds of selection, DNA aptamers that bind to the HA1 protein were isolated and shown to have different binding capacities. Among them, aptamer 10 had the strongest binding to the HA1 protein, and had an inhibitory effect on H5N1 influenza virus, as shown by the hemagglutinin and MTT assays. These results should aid the development of new drugs for the prevention and control of influenza virus infections.
Cheng
Congsheng
C
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 Yingxin Jie, Xuanwu District, 100052 Beijing, China.
Dong
Jie
J
Yao
Lihong
L
Chen
Aijun
A
Jia
Runqing
R
Huan
Lifang
L
Guo
Jianqiang
J
Shu
Yuelong
Y
Zhang
Zhiqing
Z
D004267:DNA Viruses / Q000235:genetics*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000187:drug effects / Q000502:physiology*; D007251:Influenza, Human / Q000188:drug therapy / Q000821:virology*; D052156:SELEX Aptamer Technique; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics*; D038301:Virus Inactivation / Q000187:drug effects*
D019444:Vaccines, DNA