[Study of regulation of Puerarin on blood P-selectin and the expression of aorta VCAM mRNA in diabetes].
OBJECTIVE
To observe the regulation of Puerarin on blood P-selectin and expression of aorta vascular cell adhesion molecule (VCAM) in diabetic rats induced by Streptozotocin.


METHODS
Diabetic rats were induced by intraperitoneal injection of STZ. Rats were divided into normal control group, model group, and three Puerarin groups (20, 40 and 80 mg/kg, ip) for 16 weeks. After the treatment, its cholesterol, low density lipoprotein, high density lipoprotein, P-selectin, oxidative low density lipoprotein and glycosylated low density lipoprotein. Alteration of tissue morphology via H. E staining was observed after the aorta was taken out from the rats executed by narcosis. VCAM mRNA were determined by in situ hybridization analysis.


RESULTS
(1) Diabetes mellitus and aorta lesion occurred in the four model groups. (2) Puerarin could decrease the levels of cholesterol (P < 0.05), low density lipoprotein (P < 0.05), P-selectin (P < 0.05), oxidative low density lipoprotein, and glycosylated low density (P < 0.05), lipoprotein increase high density lipoprotein (P < 0.05). The gene expression of VCAM in the aorta were significantly inhibited, and the action of Puerarin showed some dose reaction relationship at the same time (P < 0.05).


CONCLUSIONS
Puerarin have a certain in preventing aorta by inhibiting expression of adhesion molecule.
Li
Qiangxiang
Q
Ioudi Municipal Central Hospital of Hunan Provice, Loudi 417000, China. qx_l@163.com
Zhang
Xinsheng
X
Zhu
Feiyue
F
Zhang
Zhuo
Z
D000818:Animals; D001011:Aorta / Q000378:metabolism* / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D019007:P-Selectin / Q000097:blood*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*
D007529:Isoflavones; D019007:P-Selectin; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; C033607:puerarin