[Study of anticoagulant activity of ethanol extracts from leech in vitro].
OBJECTIVE
To study the anticoagulant activity of different portions of leech ethanol extracts (LEEs).


METHODS
Anticoagulant activity was determined by measuring prothrombin time(PT), thrombin time (TT), activated partial throboplstin time (APTT) and fibrinogen coagulation time (FCT).


RESULTS
PT, TT, APTT and FCT were remarkably prolonged by ethyl acetate portion of LEEs. Portions of petroleum ethrer, n-butanol and water extracted from LEEs was much weaker on anticoagulant activity.


CONCLUSIONS
The anticoagulant effect of ethyl acetate extract portion of LEEs is the strongest among the four portions, and this results may be from its inhibitory effect on thrombin-catalyzed fibrinogen hydrolysis.
Feng
Guang-jun
GJ
Institute of Pharmaceutical Science, College of Pharmaceutical Science, Southern Medical University, Guangzhou 510515, China.
Zhu
Zheng-guang
ZG
Yu
Chuan-lin
CL
Zhang
Qun
Q
Chen
Na-na
NN
Lei
Lin-sheng
LS
Wu
Shu-guang
SG
D000818:Animals; D000925:Anticoagulants / Q000302:isolation & purification / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D001780:Blood Coagulation Tests; D000431:Ethanol; D006801:Humans; D007865:Leeches / Q000737:chemistry*; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D013918:Thrombin Time
D000925:Anticoagulants; D008419:Materia Medica; D000431:Ethanol