title: Adipose expression of catalase is regulated via a novel remote PPARgamma-responsive region.
abstract: In adipose tissue of obese mice, the expression of catalase, an anti-oxidant enzyme, significantly decreases, which may cause insufficient elimination of hydrogen peroxide, but it does not in liver or skeletal muscle. However, the precise regulatory mechanism of catalase expression in adipocytes has not been fully defined. Here, we demonstrated that adipose tissues highly expressed catalase on the level comparable to liver and kidney, and treatment of mice with PPARgamma agonist significantly enhanced catalase expression in adipose tissue but not in liver. In 3T3-L1 cells, mRNA expression of catalase was up-regulated by the induction for adipose differentiation, and down-regulated by TNFalpha, in parallel with alterations in PPARgamma expression. PPARgamma agonist significantly enhanced catalase mRNA and activity. Furthermore, we newly identified a remote enhancer region containing two functional PPARgamma binding sites in mouse catalase gene. Collectively, our findings suggest that PPARgamma plays a crucial role in the expression of catalase in adipocytes.
authors: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors: Yosuke
authors: Y
authors: Okuno
authors: Morihiro
authors: M
authors: Matsuda
authors: Hironori
authors: H
authors: Kobayashi
authors: Kentaro
authors: K
authors: Morita
authors: Emi
authors: E
authors: Suzuki
authors: Atsunori
authors: A
authors: Fukuhara
authors: Ryutaro
authors: R
authors: Komuro
authors: Michio
authors: M
authors: Shimabukuro
authors: Iichiro
authors: I
authors: Shimomura
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D004789:Enzyme Activation; D005260:Female; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D009765:Obesity / Q000378:metabolism*; D009928:Organ Specificity; D047495:PPAR gamma / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D047495:PPAR gamma