F2L, a peptide derived from heme-binding protein, inhibits LL-37-induced cell proliferation and tube formation in human umbilical vein endothelial cells.
F2L, a peptide derived from heme-binding protein, was originally identified as an endogenous ligand for formyl peptide receptor-like (FPRL)2. Previously, we reported that F2L inhibits FPR and FPRL1-mediated signaling in neutrophils. Since endothelial cells express functional FPRL1, we examined the effect of F2L on LL-37 (an FPRL1 agonist)-induced signaling in human umbilical vein endothelial cells (HUVECs). F2L stimulated the chemotactic migration in HUVECs. However, F2L inhibited FPRL1 activity, resulting in the inhibition of cell proliferation and tube formation induced by LL-37 in HUVECs. We suggest that F2L will potentially be useful in the study of FPRL1 signaling and the development of drugs to treat diseases involving the FPRL1 in the vascular system.
Lee
Sun Young
SY
Department of Biochemistry, College of Medicine, Dong-A University, Busan, Republic of Korea.
Lee
Mi-Sook
MS
Lee
Ha Young
HY
Kim
Sang Doo
SD
Shim
Jae Woong
JW
Jo
Seong Ho
SH
Lee
Jae Won
JW
Kim
Joon Youn
JY
Choi
Young-Whan
YW
Baek
Suk-Hwan
SH
Ryu
Sung Ho
SH
Bae
Yoe-Sik
YS
D023181:Antimicrobial Cationic Peptides / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D010455:Peptides / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects*; D054804:Cathelicidins
D023181:Antimicrobial Cationic Peptides; D002630:Chemotactic Factors; C498127:F2L peptide, human; D010455:Peptides; D054804:Cathelicidins