Sequential conversion by catalytically active MIP and immobilized tyrosinase in a thermistor.
To amplify the heat-signal generated by MIP catalyzed solvolysis of phenylacetate the reaction has been combined for the first time in a reactor with the subsequent oxidation by immobilized tyrosinase. The polymer cleaves the substrate and the released phenol is afterwards converted to o-benzoquinone by the tyrosinase. The separated and sequentially coupled reactions are characterized by the heat generated in a thermistor. The sequential substrate conversion results in a combined heat generation which results a five times higher signal than compared to the polymer alone.
Lettau
K
K
University of Potsdam, Analytical Biochemistry, Karl-Liebknecht-Str 24-25, Potsdam-Golm, Germany.
Katterle
M
M
Warsinke
A
A
Scheller
F W
FW
D002384:Catalysis; D004800:Enzymes, Immobilized / Q000737:chemistry*; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D019800:Phenol / Q000737:chemistry*; D010648:Phenylacetates / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013817:Thermography / Q000295:instrumentation* / Q000379:methods; D014159:Transducers
D004800:Enzymes, Immobilized; D010648:Phenylacetates; D011108:Polymers; D019800:Phenol; D014442:Monophenol Monooxygenase; C025136:phenylacetic acid