Use of commercial enzyme immunoassays to detect antibodies to the herpes simplex virus type 2 glycoprotein G in a low-risk population in Hanoi, Vietnam.
Sera from 1,238 Vietnamese women in Hanoi were tested for herpes simplex virus type 2 (HSV-2). HSV-2 prevalence was 2.0%. The Kalon and Biokit assays showed significantly higher concordance to Western blotting data than did the Focus assay (P < 0.01). Screening by Focus and then retesting with Kalon/Biokit of positive samples can reduce falsely positive results significantly (P < 0.01).
Ngo
Thoai D
TD
National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland,USA.
Laeyendecker
Oliver
O
La
Hanh
H
Hogrefe
Wayne
W
Morrow
Rhoda Ashley
RA
Quinn
Thomas C
TC
D000354:Africa, Western; D000914:Antibodies, Viral / Q000097:blood*; D005189:False Positive Reactions; D005260:Female; D006561:Herpes Simplex / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D018258:Herpesvirus 2, Human / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D014744:Vietnam / Q000453:epidemiology; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic; D014759:Viral Envelope Proteins; C045464:glycoprotein G, herpes simplex virus type 2