Genotyping of HLA-I and HLA-II alleles in Chinese patients with paraneoplastic pemphigus.
BACKGROUND
Class I and class II HLA genes are thought to play a role in the immunopathogenesis of bullous dermatoses such as pemphigus vulgaris and pemphigus foliaceus, but we know little about the genetic background of paraneoplastic pemphigus (PNP) in Chinese patients.


OBJECTIVE
To identify class I and class II HLA alleles by genotyping in Chinese patients with PNP, and to find out the possible association between HLA alleles and disease susceptibility.


METHODS
Nineteen Chinese patients with PNP were enrolled in this study. HLA-A, B, C, DRB1 and DQB1 alleles were typed by polymerase chain reaction and a colour-coded sequence-specific oligonucleotide probes method.


RESULTS
The frequencies of HLA-B*4002/B*4004, B*51, B*52, Cw*14, DQB1*0301, DRB1*08 and DRB1*11 were relatively prevalent in Chinese Han patients with PNP in comparison with normal controls. After correction for multiple comparisons, Cw*14 remained statistically significant, and the other alleles were unremarkable in these patients.


CONCLUSIONS
The genetic background predisposing to PNP may be different in patients from various races and areas. HLA-Cw*14 may be the predisposing allele to PNP in Chinese patients, which is different from the predisposing allele in French patients with PNP and the alleles predisposing to pemphigus vulgaris and pemphigus foliaceus.
Liu
Q
Q
Department of Dermatology, Peking University First Hospital, Beijing 100034, China.
Bu
D-F
DF
Li
D
D
Zhu
X-J
XJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D004198:Disease Susceptibility / Q000276:immunology; D005260:Female; D015870:Gene Expression; D005805:Genes, MHC Class I / Q000235:genetics* / Q000276:immunology; D005802:Genes, MHC Class II / Q000235:genetics* / Q000276:immunology; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D008875:Middle Aged; D010257:Paraneoplastic Syndromes / Q000175:diagnosis* / Q000235:genetics*; D010392:Pemphigus / Q000175:diagnosis / Q000235:genetics*