title: Colchicine attenuates compensation to negative but not to positive lenses in young chicks.
abstract: Optic nerve-sectioned (ONS) chick eyes are capable of emmetropisation, but these eyes also exhibit increased hyperopia without any visual manipulations, which suggests altered eye growth regulation. These altered growth changes may be related to the loss of retinal ganglion cells that follows nerve lesioning. Colchicine, which also destroys retinal ganglion cells in chicks, was used to further examine the effects of retinal ganglion cell loss on emmetropisation. Growth responses of +10D and -10D lens-wearing colchicine-injected eyes were compared to those of +10D and -10D lens-wearing saline-injected eyes, respectively. Changes after removal of lenses were also analysed. Prior to lens-wear, colchicine-injected eyes exhibited longer optical axial lengths (OL; distance from cornea to retina; p=0.0185) but no differences in refractive error (RE; p=0.6588). Although myopic shifts were not significant for -10D lens-wearing colchicine-injected eyes (p=0.5913), but were for the saline-injected eyes (p=0.0034), these changes were not different (p=0.1646). However, -10D lens-induced OL changes in colchicine-injected eyes showed insignificant (p=0.2214) and reduced (p=0.0102) changes compared to those of saline-injected eyes. +10D lens-treated colchicine-injected eyes showed significant hyperopic shifts (p<0.0001) and significant reductions in OL (p<0.0001) that were similar to those of saline-injected eyes (p=0.7990 and p=0.1495, respectively). Growth responses in eyes recovering from -10D lenses were minimal, with REs unaffected (p=0.3325), but OL reductions affected (p=0.0199) by colchicine. Colchicine-injected eyes recovering from +10D lenses showed significant myopic shifts (p=0.0003) and OL elongations (p<0.0001) that were similar to those of saline-injected eyes (p=0.3999 and p=0.4731, respectively). The results showing that colchicine suppresses the ability to respond to negative lenses but leaves compensation to positive lenses relatively unchanged, are opposite to those of optic nerve sectioned eyes. We speculate that the differences are probably related to the way retinal cells are lost.
authors: University of Waterloo, Waterloo, ON, Canada. vchoh@uwaterloo.ca
authors: Vivian
authors: V
authors: Choh
authors: Varuna
authors: V
authors: Padmanabhan
authors: W S Jennifer
authors: WS
authors: Li
authors: Aaron B
authors: AB
authors: Sullivan
authors: Christine F
authors: CF
authors: Wildsoet
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000187:drug effects / Q000473:pathology; D002645:Chickens; D002829:Choroid / Q000187:drug effects / Q000473:pathology; D003078:Colchicine / Q000494:pharmacology*; D005123:Eye / Q000187:drug effects* / Q000254:growth & development*; D007908:Lens, Crystalline / Q000187:drug effects / Q000473:pathology; D007909:Lenses; D012030:Refractive Errors / Q000503:physiopathology*; D012160:Retina / Q000187:drug effects; D012165:Retinal Ganglion Cells / Q000187:drug effects; D014822:Vitreous Body / Q000187:drug effects / Q000473:pathology
chemical_list: D003078:Colchicine