The synthesis of EPR differentiable spinlabels and their coupling to uridine.
For EPR measurements of RNA, DNA, or proteins, the occurrence of the paramagnetic species is necessary. The aim of this work is to improve the synthesis of two different EPR spinlabels 2,2,6,6-tetra methyl-3,4-dehydro-piperidin-N-oxyl-4-acetylene (TEMPA) 6 and 15N-labeled TEMPA 6* and their coupling to uridine. The yield of the synthesis of TEMPA could be increased to 40% and the second nitroxide 2,2,6,6-tetramethyl-3,4-dehydro-piperidin-15N-oxyl-4-acetylene 6* could be synthesized with a yield of 11%.
Frolow
Olga
O
Johann W. Goethe Universität Frankfurt am Main, Institut für Organische Chemie und Chemische Biologie, Frankfurt am Main, Germany.
Bode
Bela E
BE
Engels
Joachim W
JW
D002851:Chromatography, High Pressure Liquid; D004578:Electron Spin Resonance Spectroscopy; D013113:Spin Labels / Q000138:chemical synthesis*; D014529:Uridine / Q000737:chemistry*
D013113:Spin Labels; D014529:Uridine