title: '1-8 interferon inducible gene family': putative colon carcinoma-associated antigens.
abstract: D(b-/-)xbeta2 microglobulin (beta2m) null mice transgenic for a chimeric HLA-A2.1/D(b)-beta2m single chain (HHD mice) are an effective biological tool to evaluate the antitumour cytotoxic T-lymphocyte response of known major histocompatibility-restricted peptide tumour-associated antigens, and to screen for putative unknown novel peptides. We utilised HHD lymphocytes to identify immunodominant epitopes of colon carcinoma overexpressed genes. We screened with HHD-derived lymphocytes over 500 HLA-A2.1-restricted peptides derived from colon carcinoma overexpressed genes. This procedure culminated in the identification of seven immunogenic peptides, three of these were derived from the 'human 1-8D gene from interferon inducible gene' (1-8D). The 1-8D gene was shown to be overexpressed in fresh tumour samples. The three 1-8D peptides were both antigenic and immunogenic in the HHD mice. The peptides induce cytotoxic T lymphocytes that were able to kill a colon carcinoma cell line HCT/HHD, in vitro and retard its growth in vivo. One of the peptides shared by all the 1-8 gene family primed efficiently normal human cytotoxic T lymphocyte precursors. These results highlight the 1-8D gene and its homologues as putative immunodominant tumour-associated antigens of colon carcinoma.
authors: Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel.
authors: B
authors: B
authors: Tirosh
authors: V
authors: V
authors: Daniel-Carmi
authors: L
authors: L
authors: Carmon
authors: A
authors: A
authors: Paz
authors: G
authors: G
authors: Lugassy
authors: E
authors: E
authors: Vadai
authors: A
authors: A
authors: Machlenkin
authors: E
authors: E
authors: Bar-Haim
authors: M-S
authors: MS
authors: Do
authors: I S
authors: IS
authors: Ahn
authors: M
authors: M
authors: Fridkin
authors: E
authors: E
authors: Tzehoval
authors: L
authors: L
authors: Eisenbach
mesh_terms: D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics* / Q000276:immunology*; D003429:Cross Reactions; D006801:Humans; D016056:Immunodominant Epitopes; D007372:Interferons / Q000737:chemistry* / Q000494:pharmacology*; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D010455:Peptides / Q000494:pharmacology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; C080081:IFITM2 protein, human; D016056:Immunodominant Epitopes; D008565:Membrane Proteins; D010455:Peptides; D007372:Interferons