Annexin A3-expressing cellular phenotypes emerge from necrotic lesion in the pericentral area in 2-acetylaminofluoren/carbon tetrachloride-treated rat livers.
Recently we found a small hepatocyte-specific protein, annexin A3 (AnxA3), in fractionated adult rat hepatocytes. Here we describe the results of an in vivo demonstration of AnxA3-expressing cellular phenotypes in the liver with 2-acetylaminofluoren (2-AAF)/carbon tetrachloride (CCl(4))-injury. In association with an elevation of alanine amino transferase (ALT) and aspartic acid amino transferase (AST) activities, hepatic AnxA3 mRNA increased markedly. AnxA3-positive cells were detected in clustered cells present in or emerging from the pericentral region. These albumin-expressed cells were histologically similar to cells expressing CD34, a hematopoietic cell marker protein. The number of clusters decreased in the days following CCl(4) treatment, and annexin-negative, but albumin-positive, oval cells appeared. We concluded that the agent-induced liver defect initially recruits bone marrow-derived cells, and that it promotes differentiation of these cells into AnxA3-positive cells, followed by emergence of the oval cells, which might have a role in the restitution of the damaged liver.
Ito
Yoshimasa
Y
Laboratory of Nutrition and Physiology, Department of Agricultural and Biological Chemistry, Nihon University College of Bioresource Sciences, Fujisawa, Kanagawa, Japan.
Watanabe
Takenori
T
Nagatomo
Shunsuke
S
Seki
Taiichiro
T
Niimi
Shingo
S
Ariga
Toyohiko
T
D015073:2-Acetylaminofluorene; D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D017318:Annexin A3 / Q000096:biosynthesis*; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism / Q000473:pathology; D002454:Cell Differentiation; D005260:Female; D022781:Hepatocytes / Q000378:metabolism*; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000473:pathology*; D009336:Necrosis; D051381:Rats; D011916:Rats, Inbred F344
D015073:2-Acetylaminofluorene; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D017318:Annexin A3