Synthesis and biological activity of phosphonocarboxylate DNA.
Oligodeoxynucleotides containing internucleotide phosphonoacetate esters are taken up irreversibly by cells in culture in the absence of cationic lipids. These oligonucleotides also are active in stimulating RNase H and are stable toward nucleases.
Yamada
Christina M
CM
Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, USA. marvin.caruthers@colorado.edu
Dellinger
Douglas J
DJ
Caruthers
Marvin H
MH
D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D004951:Esterification; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D008055:Lipids / Q000737:chemistry; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D010746:Phosphonoacetic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D014162:Transfection
D008055:Lipids; D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides; D012313:RNA; D004247:DNA; D010746:Phosphonoacetic Acid