CD30 supports lung inflammation.
The physiological functions of CD30 have not been fully elucidated. Here we show that in CD30-deficient mice (CD30(-/-)), lung inflammation is significantly diminished in the ovalbumin (OVA) model of airway hyperreactivity. In CD30(-/-) mice, the recruitment of eosinophils into the airways after OVA-aerosol challenge of OVA-primed mice was significantly diminished when compared with wild-type (w.t.) mice. IL-13 levels were also significantly reduced in CD30(-/-) mice while levels of IFN-gamma, IL-4, IL-5 and IgE in bronchoalveolar lavage fluid, lung tissue and serum were comparable to w.t. mice. Peribronchial lymph node cells from CD30(-/-) mice, re-stimulated in vitro with OVA, secreted significantly lower levels of IL-13 than those from w.t. mice, but showed normal proliferative response and other cytokine production. Exogenous IL-13 reconstituted airway recruitment of leukocytes in OVA-challenged CD3O(-/-) mice. Adoptive transfer to naive w.t. mice of in vitro OVA-re-stimulated spleen cells from CD30(-/-) mice failed to induce eosinophilic pulmonary inflammation in contrast to transfer of primed cells from w.t. mice. These results indicate that CD30 is a regulator of T(h)2 responses in the effector-memory phase and a regulator of IL-13 production in memory cells in the lung.
Nam
Sang-Yun
SY
Department of Biological Science, School of Science and Technology, Jeonju University, Jeonju 560-759, Korea.
Kim
Young-Hyun
YH
Do
Jeong-Su
JS
Choi
Yun-Hwa
YH
Seo
Hyo-Jung
HJ
Yi
Ho-Keun
HK
Hwang
Pyung-Han
PH
Song
Chang-Ho
CH
Lee
Hern-Ku
HK
Kim
Jeong-Su
JS
Podack
Eckhard R
ER
D000818:Animals; D004195:Disease Models, Animal; D004804:Eosinophils / Q000166:cytology / Q000276:immunology; D006801:Humans; D007156:Immunologic Memory; D018793:Interleukin-13 / Q000096:biosynthesis* / Q000235:genetics; D017730:Ki-1 Antigen / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D011014:Pneumonia / Q000276:immunology* / Q000503:physiopathology*; D018418:Th2 Cells / Q000276:immunology
D018793:Interleukin-13; D017730:Ki-1 Antigen; D010047:Ovalbumin