title: Molecular characterisation and phylogenetic analysis of Chronic bee paralysis virus, a honey bee virus.
abstract: The complete sequences of the two major RNAs of Chronic bee paralysis virus (CBPV) have been determined. RNA 1 (3674nt long) and RNA 2 (2305nt long) are positive single-stranded RNAs that are capped but not polyadenylated. The 3' ends of both RNAs are unreactive to polymerisation or ligation even in denaturing conditions, a feature already observed in alphanodavirus RNAs. The three previously described smaller RNAs [Overton, H.A., Buck, K.W., Bailey, L., et al., 1982. Relationships between the RNA components of Chronic bee-paralysis virus and those of chronic bee-paralysis virus associate. J. Gen. Virol. 63, 171-179], were not detected in this study, supporting the hypothesis that they would correspond to the three RNAs of the Chronic bee paralysis satellite virus (CBPSV). RNA 1 and RNA 2 encoded three and four overlapping open reading frames (ORFs), respectively. The amino acid sequences deduced from the ORF 3 on RNA 1 shared the conserved motifs of the RNA-dependent RNA polymerase (RdRp) sequence and presented similarities with members of the Nodaviridae and Tombusviridae families. However, no similarities were found between the other CBPV deduced amino acid sequences and sequences in the NCBI databases, suggesting that CBPV is the prototype of a new family of positive single-stranded RNA viruses.
authors: Agence Française de Sécurité Sanitaire des Aliments, 105 route des Chappes, BP 111, 06 902 Sophia-Antipolis, France.
authors: Violaine
authors: V
authors: Olivier
authors: Philippe
authors: P
authors: Blanchard
authors: Soraya
authors: S
authors: Chaouch
authors: Perrine
authors: P
authors: Lallemand
authors: Frank
authors: F
authors: Schurr
authors: Olivier
authors: O
authors: Celle
authors: Eric
authors: E
authors: Dubois
authors: Noël
authors: N
authors: Tordo
authors: Richard
authors: R
authors: Thiéry
authors: Rémi
authors: R
authors: Houlgatte
authors: Magali
authors: M
authors: Ribière
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000821:virology*; D016679:Genome, Viral; D007304:Insect Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D030381:Nodaviridae / Q000235:genetics; D016366:Open Reading Frames; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D012367:RNA, Viral / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D019183:Tombusviridae / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D014713:Viruses, Unclassified / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase