Protein partitioning in poly(ethylene glycol)/sodium polyacrylate aqueous two-phase systems.
The partition of hemoglobin, lysozyme and glucose-6-phosphate dehydrogenase (G6PDH) in a novel inexpensive aqueous two-phase system (ATPS) composed by poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA) has been studied. The effect of NaCl and Na(2)SO(4), pH and PEG molecular size on the partitioning has been studied. At high pH (above 9), hemoglobin partitions strongly to the PEG-phase. Although some precipitation of hemoglobin occurs, high recovery values are obtained particularly for lysozyme and G6PDH. The partitioning forces are dominated by the hydrophobic and electrochemical (salt) effects, since the positively charged lysozyme and negatively charged G6PDH partitions to the non-charged PEG and the strongly negatively charged polyacrylate enriched phase, respectively.
Johansson
Hans-Olof
HO
Department of Biochemical and Pharmaceutical Technology, University of São Paulo, Av. Prof. Lineu Prestes, 580 Bloco 16, Cidade Universitaria, 05508-000 São Paulo, Brazil. Hans-Olof.johansson@biochemistry.lu.se
Magaldi
Flavio Musa
FM
Feitosa
Eloi
E
Pessoa
Adalberto
A
D000180:Acrylic Resins; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis / Q000302:isolation & purification*; D006454:Hemoglobins / Q000032:analysis / Q000302:isolation & purification*; D009113:Muramidase / Q000032:analysis / Q000302:isolation & purification*; D011092:Polyethylene Glycols
D000180:Acrylic Resins; D006454:Hemoglobins; D011092:Polyethylene Glycols; C006903:carbopol 940; D005954:Glucosephosphate Dehydrogenase; D009113:Muramidase