The production and crystallization of the human leukocyte antigen class II molecules HLA-DQ2 and HLA-DQ8 complexed with deamidated gliadin peptides implicated in coeliac disease.
The major histocompatibility complex (MHC) class II molecules HLA-DQ2 and HLA-DQ8 are key risk factors in coeliac disease, as they bind deamidated gluten peptides that are subsequently recognized by CD4+ T cells. Here, the production and crystallization of both HLA-DQ2 and HLA-DQ8 in complex with the deamidated gliadin peptides DQ2 alpha-I (PQPELPYPQ) and DQ8 alpha-I (EGSFQPSQE), respectively, are reported.
Henderson
Kate N
KN
The Protein Crystallography Unit, Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria 3800, Australia.
Reid
Hugh H
HH
Borg
Natalie A
NA
Broughton
Sophie E
SE
Huyton
Trevor
T
Anderson
Robert P
RP
McCluskey
James
J
Rossjohn
Jamie
J
D002446:Celiac Disease / Q000378:metabolism*; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D003641:Deamination; D005903:Gliadin / Q000737:chemistry / Q000378:metabolism*; D006683:HLA-DQ Antigens / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007962:Leukocytes / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding
D006683:HLA-DQ Antigens; C053186:HLA-DQ2 antigen; C109756:HLA-DQ8 antigen; D010455:Peptides; D005903:Gliadin