Ferrocenylnaphthalene diimide-based electrochemical ribonuclease assay.
Messenger RNA (mRNA) poly(A)+RNA (from mouse kidney) was immobilized on a N-hydroxysuccinimide(NHS)-activated carboxylic acid modified electrode prepared by the treatment of a gold electrode with 3,3'-dithiodipropionic acid, followed by NHS and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). An electrochemical measurement using this mRNA electrode was carried out in an electrolyte containing ferrocenylnaphthalene diimide (1), and showed an electrochemical signal based on 1 concentrated on immobilized mRNA. After treating this electrode with water containing varied amounts of ribonuclease A (RNase A), the current peak based on 1 decreased with increasing in the amount of RNase A with a linear correlation in the range of 0.2-10 pg of RNase A.
Kanazawa
Masanori
M
Department of Applied Chemistry, Kyushu Institute of Technology, Kitakyushu, Fukuoka 840-8550, Japan.
Sato
Shinobu
S
Ohtsuka
Keiichi
K
Takenaka
Shigeori
S
D000818:Animals; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D005296:Ferrous Compounds / Q000737:chemistry*; D006046:Gold; D007094:Imides / Q000737:chemistry*; D007202:Indicators and Reagents; D007668:Kidney / Q000737:chemistry; D051379:Mice; D008839:Microelectrodes; D010084:Oxidation-Reduction; D011061:Poly A / Q000737:chemistry; D012333:RNA, Messenger / Q000737:chemistry; D012259:Ribonuclease, Pancreatic / Q000032:analysis*
D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D007094:Imides; D007202:Indicators and Reagents; D012333:RNA, Messenger; C406363:ferrocenylnaphthalene diimide; D011061:Poly A; D006046:Gold; D012259:Ribonuclease, Pancreatic