title: Simultaneous determination of blood glucose and isoleucine levels in rats after chronic alcohol exposure by microwave-assisted derivatization and isotope dilution gas chromatography/mass spectrometry.
abstract: Blood glucose and isoleucine are two biomarkers of chronic alcohol exposure. Simultaneous determination of blood glucose and isoleucine levels helps to illuminate the influence of alcohol on the metabolism of glucose and amino acids. The most accurate method for the detection of serum glucose is isotope dilution gas chromatography/mass spectrometry (ID GC/MS). In this study, a rapid, simple and sensitive technique was developed for the quantitative analysis of glucose and isoleucine in rats after chronic alcohol exposure by microwave-assisted derivatization (MAD) and ID GC/MS. Serum glucose and isoleucine were rapidly derivatized by N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) with microwave irradiation, and the trimethylsilyl derivatives were analyzed by GC/MS. This technique was used to demonstrate that pyrroloquinoline quinone (PQQ), a non-covalently bound prosthetic group in some quinoproteins involved in the metabolism of some sugar or alcohol, could reverse alcohol exposure induced glucose elevation. On the other hand, it did not affect the metabolism of isoleucine whose level was elevated along with serum glucose. The combination of MAD and ID GC/MS has been shown to be an accurate, rapid, simple and sensitive method for the quantification of glucose and isoleucine in serum samples.
authors: Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.
authors: Ruyi
authors: R
authors: Xue
authors: Si
authors: S
authors: Zhang
authors: Chunhui
authors: C
authors: Deng
authors: Ling
authors: L
authors: Dong
authors: Taotao
authors: T
authors: Liu
authors: Jiyao
authors: J
authors: Wang
authors: Hongyi
authors: H
authors: Wu
authors: Jianxin
authors: J
authors: Gu
authors: Xizhong
authors: X
authors: Shen
mesh_terms: D000284:Administration, Oral; D000437:Alcoholism / Q000097:blood* / Q000209:etiology; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001786:Blood Glucose / Q000032:analysis*; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D000431:Ethanol / Q000633:toxicity*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007532:Isoleucine / Q000097:blood*; D007553:Isotope Labeling; D008297:Male; D008872:Microwaves; D011758:Pyrroles / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D011758:Pyrroles; D011804:Quinolines; C410406:pyrroloquinoline; D007532:Isoleucine; D000431:Ethanol