[Using direct enzyme linked immunosorbent assay for the detection of IgG antibody on Bartonella henselae among healthy people in Changping, Beijing].
OBJECTIVE
To detect Bartonella henselae IgG antibody among healthy people in Changping, Beijing.


METHODS
Using indirect enzyme linked immunosorbent assay (ELISA) and immunofluorescence antibody assay (IFA) to detect IgG antibody of Bartonella henselae among human beings.


RESULTS
The sensitivity and specificity of ELISA were 70.6% and 91.6% respectively, with the positive predictive value of serological test as 82.2%, and the negative predictive value as 84.9%, based on results of IFA. The positive rate was 34.5% among 357 healthy people on indirect ELISA but was 35.6% among 239 people with IFA.


CONCLUSIONS
The results indicated that the indirect ELISA was a very quick, sensitive and available method for detecting Bartonella henselae in human beings, as well as a high positive percent age of Bartonella henselae among the healthy people of Changping Beijing.
Yang
Xiao-Ran
XR
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Liu
Qi-Yong
QY
Cui
Bu-Yun
BY
Wang
Lian-Xiu
LX
Peng
Zhi-Hui
ZH
Ren
Dong-Sheng
DS
D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000276:immunology*; D018416:Bartonella henselae / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G