Preparation of 2'-hydrazino oligonucleotides and their reaction with aldehydes and 1,3-diketones.
Oligodeoxyribonucleotides that contain a hydrazino nucleoside, 2'-O-(2-hydrazinoethyl)uridine were prepared and shown to react with aldehydes or 1,3-diketones with the formation of hydrazones or pyrazoles, respectively. The method may be applicable for the preparation of oligonucleotide-peptide conjugates.
Zatsepin
Timofei S
TS
Department of Chemistry and A. N. Belozersky Institute of Physico-Chemical Biology, M. V. Lomonossov Moscow State University, Moscow, Russia.
Oretskaya
Tatiana S
TS
Gait
Michael J
MJ
Stetsenko
Dmitry A
DA
D000447:Aldehydes / Q000737:chemistry*; D001483:Base Sequence; D006834:Hydrazines / Q000138:chemical synthesis*; D007659:Ketones / Q000737:chemistry*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000447:Aldehydes; D006834:Hydrazines; D007659:Ketones; D009841:Oligonucleotides