High-performance liquid chromatography analysis of ten dyes for control of safety of commercial articles.
Two high-performance liquid chromatographic methods, with ultraviolet-visible spectrophotometry detection (HPLC-UV/Vis) and with tandem mass spectrometry triple quadrupole interfaced with positive ion mode electrospray ionization detection (HPLC-ESI+-QqQ-MS/MS), for determination and quantification of ten commercial dyes are proposed for control in commercial products. Multiple peaks observed for some of the studied dyes in HPLC-UV/Vis chromatograms forced to obtain structural information by HPLC-ESI-MS/MS method with scan mode. The quality parameters of the two proposed chromatographic methods were evaluated for different requirements of normative, showing detection (LODs) and quantification (LOQs) limits around 60-890 and 200-2990 microg L(-1) for HPLC-UV/Vis, and 4.54-14.3 and 15.0-47.6 microg L(-1) for HPLC-ESI+-QqQ-MS/MS.
Noguerol-Cal
R
R
Laboratorio de Química, Centro de Investigacións Tecnolóxicas, UDC-Campus de Esteiro s/n, 15403 Ferrol, Spain. iquimica@cdf.udc.es
López-Vilariño
J M
JM
Fernández-Martínez
G
G
Barral-Losada
L
L
González-Rodríguez
M V
MV
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004396:Coloring Agents / Q000032:analysis* / Q000633:toxicity; D008420:Manufactured Materials / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D053719:Tandem Mass Spectrometry
D004396:Coloring Agents