Genetic characterization of two S-adenosylmethionine-induced ABC transporters reveals their roles in modulations of secondary metabolism and sporulation in Streptomyces coelicolor M145.
S-Adenosylmethionine (SAM) was previously documented to activate secondary metabolism in a variety of Streptomyces spp. and to promote actinorhodin (ACT) and undecylprodigiosin (RED) in Streptomyces coelicolor. The SAM-induced proteins in S. coelicolor include several ABC transporter components (SCO5260 and SCO5477) including BldKB, the component of a well-known regulatory factor for differentiations. In order to assess the role of these ABC transporter complexes in differentiation of Streptomyces, SCO5260 and SCO5476, the first genes from the cognate complex clusters, were individually inactivated by gene replacement. Inactivation of either SCO5260 or SCO5476 led to impaired sporulation on agar medium, with the more drastic defect in the SCO5260 null mutant (ASCO5260). ASCO5260 displayed growth retardation and reduced yields of ACT and RED in liquid cultures. In addition, SAM supplementation failed in promoting the production of ACT and RED in ASCO5260. Inactivation of SCO5476 gave no significant change in growth and production of ACT and RED, but impaired the promoting effect of SAM on ACT production without interfering with the effect on RED production. The present study suggests that SAM induces several ABC transporters to modulate secondary metabolism and morphological development in S. coelicolor.
Shin
Su-Kyoung
SK
Department of Biological Science, Institute of Bioscience and Biotechnology, Myongji University, Yongin 449-728, Korea.
Park
Hyun-Suh
HS
Kwon
Hyung-Jin
HJ
Yoon
Hyun-Jin
HJ
Suh
Joo-Won
JW
D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000502:physiology*; D012436:S-Adenosylmethionine / Q000494:pharmacology*; D013171:Spores, Bacterial / Q000502:physiology; D048372:Streptomyces coelicolor / Q000502:physiology*
D018528:ATP-Binding Cassette Transporters; D012436:S-Adenosylmethionine