title: Analysis of 5-methylcytosine in DNA of breast and colon cancer tissues.
abstract: 5-methylcytosine (m(5)C) can be used as a sensitive marker of progress of the tumor formation induced by the oxidative damage reactions. We have analyzed the amount of m(5)C in DNA of patients with breast and colon cancers. Two dimensional thin layer chromatography (TLC) has been used to monitor 5-methylcytosine level in DNA extracted from cancer tissues. The level of methylation of cytosine at C-5 position in DNA from breast cancer patients correlates well with the malignancy of tumors. Interestingly higher amount of m(5)C in DNA for the breast cancer patients treated with different chemotherapeutics was observed. It suggests an activation of DNA methyltransferase as well as a genomic suppression of the DNA repair genes expression. These differences clearly reflect the health condition of patients and support the global analysis of m(5)C in DNA as a good marker for diagnosis of neoplasia in clinical practice.
authors: Department and Clinic of Neurosurgery and Neurotraumatology, Karol Marcinkowski University of Medical Sciences, Poznan, Poland.
authors: Anna-Maria
authors: AM
authors: Barciszewska
authors: Dawid
authors: D
authors: Murawa
authors: Iwona
authors: I
authors: Gawronska
authors: Pawel
authors: P
authors: Murawa
authors: Stanislaw
authors: S
authors: Nowak
authors: Miroslawa Z
authors: MZ
authors: Barciszewska
mesh_terms: D044503:5-Methylcytosine / Q000032:analysis*; D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000737:chemistry* / Q000235:genetics; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000235:genetics; D004249:DNA Damage; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000737:chemistry* / Q000235:genetics; D044127:Epigenesis, Genetic; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction
chemical_list: D004273:DNA, Neoplasm; D044503:5-Methylcytosine