title: Sequence-specific recognition of double-stranded DNA by synthetic minor groove binder conjugates. toward the construction of artificial site-specific deoxyribonucleases.
abstract: Bis-conjugates of hairpin N-methylpyrrole/N-methylimidazole oligocarboxamide minor groove binders (MGB) possessing enhanced affinity and sequence-specificity for dsDNA were synthesized. Two hairpin MGBs were connected by their N-termini via an aminodiacetate linker. The binding of bis-MGB conjugates to the target DNA was studied by gel mobility retardation, footprinting, and circular dichroism; their affinity and binding mode in the DNA minor groove were determined. In order to functionalize the bis-MGB conjugates, DNA-cleaving agents such as phenanthroline or bipyridine were attached. Effective site-specific cleavage of target DNA in the presence of Cu(2+) ions was observed.
authors: Muséum National d'Histoire Naturelle, INSERM U565, Paris, France. alexandre.boutorine@mnhn.fr
authors: Alexandre S
authors: AS
authors: Boutorine
authors: Ludovic
authors: L
authors: Halby
authors: Philippe
authors: P
authors: Simon
authors: Loïc
authors: L
authors: Perrouault
authors: Carine
authors: C
authors: Giovannangeli
authors: Georgy V
authors: GV
authors: Gursky
authors: Anna N
authors: AN
authors: Surovaya
authors: Sergueï L
authors: SL
authors: Grokhovsky
authors: Vladimir A
authors: VA
authors: Ryabinin
authors: Alexandre N
authors: AN
authors: Sinyakov
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D003851:Deoxyribonucleases / Q000737:chemistry*; D007093:Imidazoles / Q000138:chemical synthesis / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D010618:Phenanthrolines / Q000737:chemistry; D011758:Pyrroles / Q000138:chemical synthesis / Q000737:chemistry*; D016337:RNA, Catalytic / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D007093:Imidazoles; D010618:Phenanthrolines; D011758:Pyrroles; D016337:RNA, Catalytic; D004247:DNA; D003851:Deoxyribonucleases; C096654:N-methylpyrrole