Identification of enterovirus serotypes by pyrosequencing using multiple sequencing primers.
Human enteroviruses (HEV) are considered as one of the major causes of central nervous system infections in pediatrics. They are currently classified into five species involving more than 60 officially recognized serotypes. This study describes a rapid molecular method, based on pyrosequencing of a VP1 fragment, for the identification of enterovirus serotypes. In order to do so, 200 isolates and clinical specimens that were first grouped into 62 different HEV serotypes using neutralization test, were analyzed by pyrosequencing. All serotypes were identified using the proposed method. Most of the isolates previously untypeable by classical procedures, as well as mixed enterovirus infections containing viruses belonging to different species, could also be determined using pyrosequencing. The present results give support to pyrosequencing as an efficient method of HEV genotyping.
Silva
Paula Andreia
PA
Robert Koch-Institute, Nordufer 20, 13353 Berlin, Germany. SilvaP@rki.de <SilvaP@rki.de>
Diedrich
Sabine
S
de Paula Cardoso
Divina das Dores
Dd
Schreier
Eckart
E
D000595:Amino Acid Sequence; D004770:Enterovirus / Q000145:classification* / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis / Q000821:virology*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics*; D016415:Sequence Alignment; D017421:Sequence Analysis
D012367:RNA, Viral