Culture in vitro modulation of human leukocyte antigen molecules and costimulatory molecules on human retinal pigment epithelium.
OBJECTIVE
To determine the potential of culture in vitro to alter the human leukocyte antigen (HLA) molecules and costimulatory molecules on human retinal pigment epithelium (RPE).


METHODS
Pure RPE were isolated and cultured. Two sets of RPE (normal and activated) were used. Activated RPE were obtained by incubating primary cultures of RPE with recombinant human interferon-gamma. The expression of HLA molecules and costimulatory molecules on human RPE at different passages after culture in vitro were analyzed quantitatively by flow cytometry.


RESULTS
In the process of routine culture on culture flask, the duration of culture in vitro was significantly correlated with the expression of HLA-ABC molecules on the normal RPE and the activated RPE (r = -0.893, p < 0.001 and r = -0.964, p < 0.001 respectively), HLA-DR molecule on the activated RPE (r = -0.901, p < 0.001) and intercellular adhesion molecule-1 on the normal RPE (r = 0.961, p < 0.001). There were no correlations between the duration of culture in vitro and the expression of HLA-DR molecule on the normal RPE, intercellular adhesion molecule-1 on the activated RPE, B7-1 and B7-2 molecules on the normal RPE and the activated RPE.


CONCLUSIONS
The chronic rejection is the major immunological rejection following RPE allogeneic graft. Culture in vitro modulation of HLA molecules and costimulatory molecules on RPE may increase lymphocyte infiltration and the activated RPE.
Xie
Mao-Song
MS
Department of Ophthalmology, School of Medicine, Shandong University, Jinan, China.
Xu
Guo-Xing
GX
D000954:Antigens, Surface / Q000378:metabolism*; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D002478:Cells, Cultured; D005434:Flow Cytometry; D006084:Graft Rejection / Q000276:immunology; D006680:HLA Antigens / Q000378:metabolism*; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000276:immunology; D008218:Lymphocytosis / Q000276:immunology; D010857:Pigment Epithelium of Eye / Q000187:drug effects / Q000276:immunology / Q000502:physiology* / Q000637:transplantation; D011994:Recombinant Proteins / Q000494:pharmacology
D000954:Antigens, Surface; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D006680:HLA Antigens; D006684:HLA-DR Antigens; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma