Genetic transformation of barley microspores using anther bombardment.
Bombardment of intact anthers of commercial barley (Hordeum vulgare) varieties resulted in 0.5-1.0% of transformed microspores of which 20-40% continued in androgenic development (0.2% of all bombarded microspores). Using a system based on bombardment of anthers is therefore likely to be more technically efficient than the use of a microspore isolation, transformation and regeneration system. Bombardment of anthers has a number of technical and scientific advantages over existing systems for gene transfer and can be considered as a alternative method to existing methods for genetic transformation in barley.
Obert
Bohus
B
Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, Scotland, UK. obert@savba.sk
Middlefell-Williams
Jill
J
Millam
Steve
S
D019470:Biolistics / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D003080:Cold Temperature; D035264:Flowers / Q000235:genetics*; D017930:Genes, Reporter; D001467:Hordeum / Q000235:genetics*; D008353:Mannitol / Q000737:chemistry; D030821:Plants, Genetically Modified / Q000235:genetics*; D010957:Plasmids; D011312:Pressure; D014170:Transformation, Genetic
D008353:Mannitol