Heterologous expression of SARS-CoV ORF10 and X5 genes in E. coli and Streptomyces lividans TK24.
In previous studies a variety of novel accessory genes has been identified that were interspersed among the structural genes of the SARS-CoV (severe acute respiratory syndrome coronavirus) genome. The predicted unknown proteins (PUPs) encoded by the accessory genes, which are considered to be unique to the SARS-CoV genome, might play important roles in the SARS-CoV infection. Two of these genes, called ORF10 and X5, were synthesized and introduced into E. coli and Streptomyces lividans TK24, respectively. SDS-PAGE and Western blot revealed that the ORF10 and X5 genes have been expressed in the two hosts. This is the first report of heterologous expression of ORF10 and X5 genes in E. coli and S. lividans TK24. This work makes it possible to study the structure and potential functions of proteins encoding by these two genes.
Kong
Jiang-qiang
JQ
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
Wang
Wei
W
Du
Guan-hua
GH
Zhu
Ping
P
Cheng
Ke-di
KD
D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D005822:Genetic Vectors; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000302:isolation & purification; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics*; D048170:Streptomyces lividans / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification
D004279:DNA, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins