Regeneration of dentin-pulp complex with cementum and periodontal ligament formation using dental bud cells in gelatin-chondroitin-hyaluronan tri-copolymer scaffold in swine.
The purpose of this study is to use a tissue engineering approach for tooth regeneration. The swine dental bud cells (DBCs) were isolated from the developing mandibular teeth, expanded in vitro, and cultured onto cylinder scaffold gelatin-chrondroitin-hyaluronan-tri-copolymer (GCHT). After culturing in vitro, the DBCs/GCHT scaffold was autografted back into the original alveolar socket. Hematoxylin and eosin (H&E) staining combined with immunohistochemical staining were applied for identification of regenerated tooth structure. After 36-week post-transplantation, tooth-like structures, including well-organized dentin-pulp complex, cementum, and periodontal ligament, were evident in situ in two of six experimental animals. The size of the tooth structure (1 x 0.5 x 0.5 cm(3) and 0.5 x 0.5 x 0.5 cm(3) size) appeared to be dictated by the size of the GCHT scaffold (1 x 1 x 1.5 cm(3)). The third swine was demonstrated with irregular dentin-bony like calcified tissue about 1 cm in diameter without organized tooth or periodontal ligament formation. The other three swine in the experimental group showed normal bone formation and no tooth regeneration in the transplantation sites. The successful rate of tooth regeneration from DBCs/GCHT scaffolds' was about 33.3%. In the control group, three swine's molar teeth buds were removed without DBCs/GCHT implantation, the other three swine received GCHT scaffold implants without DBCs. After evaluation, no regenerated tooth was found in the transplantation site of the control group. The current results using DBSs/GCHT scaffold autotransplantation suggest a technical breakthrough for tooth regeneration.
Kuo
Tzong-Fu
TF
Graduate Institute of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
Huang
An-Ting
AT
Chang
Hao-Hueng
HH
Lin
Feng-Huei
FH
Chen
San-Tai
ST
Chen
Rung-Shu
RS
Chou
Cheng-Hung
CH
Lin
Hsin-Chi
HC
Chiang
Han
H
Chen
Min-Huey
MH
D000818:Animals; D002478:Cells, Cultured; D002807:Chondroitin / Q000494:pharmacology / Q000648:ultrastructure; D003739:Dental Cementum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D003804:Dentin / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D005780:Gelatin / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000494:pharmacology / Q000648:ultrastructure; D007150:Immunohistochemistry; D007568:Jaw / Q000000981:diagnostic imaging; D056948:Orthognathic Surgical Procedures; D010513:Periodontal Ligament / Q000187:drug effects / Q000378:metabolism*; D011859:Radiography; D012038:Regeneration / Q000187:drug effects*; D013194:Staining and Labeling; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014070:Tooth / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure
D005780:Gelatin; D006820:Hyaluronic Acid; D002807:Chondroitin