Direct selection and phage display of a Gram-positive secretome.
Surface, secreted and transmembrane protein-encoding open reading frames, collectively the secretome, can be identified in bacterial genome sequences using bioinformatics. However, functional analysis of translated secretomes is possible only if many secretome proteins are expressed and purified individually. We have now developed and applied a phage display system for direct selection, identification, expression and purification of bacterial secretome proteins.
Jankovic
Dragana
D
Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand. d.jankovic@massey.ac.nz
Collett
Michael A
MA
Lubbers
Mark W
MW
Rakonjac
Jasna
J
D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D052201:Lacticaseibacillus rhamnosus / Q000737:chemistry* / Q000235:genetics; D016366:Open Reading Frames; D019151:Peptide Library; D021382:Protein Sorting Signals; D040901:Proteomics / Q000379:methods*
D001426:Bacterial Proteins; D019151:Peptide Library; D021382:Protein Sorting Signals