title: Serine-7 of the RNA polymerase II CTD is specifically required for snRNA gene expression.
abstract: RNA polymerase II (Pol II) transcribes genes that encode proteins and noncoding small nuclear RNAs (snRNAs). The carboxyl-terminal repeat domain (CTD) of the largest subunit of mammalian RNA Pol II, comprising tandem repeats of the heptapeptide consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7, is required for expression of both gene types. We show that mutation of serine-7 to alanine causes a specific defect in snRNA gene expression. We also present evidence that phosphorylation of serine-7 facilitates interaction with the snRNA gene-specific Integrator complex. These findings assign a biological function to this amino acid and highlight a gene type-specific requirement for a residue within the CTD heptapeptide, supporting the existence of a CTD code.
authors: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
authors: Sylvain
authors: S
authors: Egloff
authors: Dawn
authors: D
authors: O'Reilly
authors: Rob D
authors: RD
authors: Chapman
authors: Alice
authors: A
authors: Taylor
authors: Katrin
authors: K
authors: Tanzhaus
authors: Laura
authors: L
authors: Pitts
authors: Dirk
authors: D
authors: Eick
authors: Shona
authors: S
authors: Murphy
mesh_terms: D000409:Alanine; D000595:Amino Acid Sequence; D002460:Cell Line; D016384:Consensus Sequence; D005786:Gene Expression Regulation; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000235:genetics; D006801:Humans; D009154:Mutation; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012319:RNA Polymerase II / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012342:RNA, Small Nuclear / Q000235:genetics*; D012694:Serine / Q000378:metabolism*; D013698:Templates, Genetic; D014158:Transcription, Genetic
chemical_list: D034441:Heterogeneous-Nuclear Ribonucleoproteins; D009842:Oligopeptides; D021122:Protein Subunits; D012333:RNA, Messenger; D012342:RNA, Small Nuclear; C405484:U2 small nuclear RNA; D012694:Serine; D012319:RNA Polymerase II; D000409:Alanine