title: Bone regeneration of dental implant dehiscence defects using a cultured periosteum membrane.
abstract: OBJECTIVE
  This study aimed to demonstrate the feasibility of a cultured periosteum (CP) membrane for use in guided bone regeneration at sites of implant dehiscence.
  
  
  METHODS
  Four healthy beagle dogs were used in this study. Implant dehiscence defects (4 x 4 x 3 mm) were surgically created at mandibular premolar sites where premolars had been extracted 3 months back. Dental implants (3.75 mm in diameter and 7 mm in length) with machined surfaces were placed into the defect sites (14 implants in total). Each dehiscence defective implant was randomly assigned to one of the following two groups: (1) PRP gel without cells (control) or (2) a periosteum membrane cultured on PRP gel (experimental). Dogs were killed 12 weeks after operation and nondecalcified histological sections were made for histomorphometric analyses including percent linear bone fill (LF) and bone-to-implant contact (BIC).
  
  
  RESULTS
  Bone regeneration in the treatment group with a CP membrane was significantly greater than that in the control group and was confirmed by LF analysis. LF values in the experimental and the control groups were 72.36+/-3.14% and 37.03+/-4.63%, respectively (P<0.05). The BIC values in both groups were not significantly different from each other. The BIC values in the experimental and the control groups were 40.76+/-10.30% and 30.58+/-9.69%, respectively (P=0.25) and were similar to native bone.
  
  
  CONCLUSIONS
  This study demonstrated the feasibility of a CP membrane to regenerate bone at implant dehiscence defect.
authors: Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Daiki
authors: D
authors: Mizuno
authors: Hideaki
authors: H
authors: Kagami
authors: Hirokazu
authors: H
authors: Mizuno
authors: Junji
authors: J
authors: Mase
authors: Kazutada
authors: K
authors: Usami
authors: Minoru
authors: M
authors: Ueda
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D003758:Dental Implantation, Endosseous / Q000009:adverse effects*; D015921:Dental Implants; D004285:Dogs; D005240:Feasibility Studies; D005260:Female; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D020007:Implants, Experimental; D008567:Membranes, Artificial; D010521:Periosteum; D053657:Platelet-Rich Plasma; D011897:Random Allocation; D013529:Surgical Wound Dehiscence / Q000209:etiology / Q000628:therapy*; D046509:Tissue Culture Techniques
chemical_list: D015921:Dental Implants; D008567:Membranes, Artificial