title: Protein quantitation in 2-D gels using fluorescence with water Raman as an internal standard.
abstract: We have developed a method to enhance fluorescence quantification in two-dimensional gel electrophoresis using the inherent Raman scattering of water as an internal standard. We demonstrate this water internal standard (WIS) method using quantitative comparisons of commercially available protein standards that were either covalently tagged or passively stained with fluorescent tags. Thus, WIS opens up the possibility of enhancing intra- and intergel quantitative comparisons.
authors: Department of Chemistry, Purdue University, West Lafayette, Indiana, USA.
authors: Yvette L
authors: YL
authors: Loethen
authors: Giselle M
authors: GM
authors: Knudsen
authors: Brandon
authors: B
authors: Davis
authors: Ravindra
authors: R
authors: Gudihal
authors: V Jo
authors: VJ
authors: Davisson
authors: Dor
authors: D
authors: Ben-Amotz
mesh_terms: D002138:Calibration; D011506:Proteins / Q000032:analysis*; D012015:Reference Standards; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014867:Water
chemical_list: D011506:Proteins; D014867:Water