[The influence of oxygen and glucose deprivation on the expression of neurite growth inhibitor Nogo-A in oligodendrocyte precursor cell of rat].
OBJECTIVE
To investigate the Nogo-A expression and its regeneration inhibition role in oligodendrocyte precursor cells (OLPs) after oxygen & glucose deprivation (OGD).


METHODS
The OLPs were separated by the improved procedure of separation and purification through agitation and then cultured in chemically defined medium. The OGD model of OLPs was set up by using Na2S2O4 and Earle's fluid in the medium in vitro. Immunofluorescence assay was applied to identify the OLPs with its specific antibodies such as A2B5, O4 and O1. Western blot and immunofluorescence assay were used to analyze the expression of Nogo-A in OLPs at 10 min, 30 min and 60 min after OGD. The livability rate of cells in each group was detected by MTT.


RESULTS
The expression of Nogo-A was increased significantly compared with control at 10 min, 30 min and 60 min after OGD (P < 0.05). MTT result showed that the livability rate of OLPs with OGD was significantly lower than that of control (P < 0.05).


CONCLUSIONS
The expression of Nogo-A is increased while the OLPs livability is dropping down after OGD, which suggests that Nogo-A may play a role in OLPs damage and in the pathogenesis of restraining OLPs' regeneration.
Tang
Jun
J
Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu, China.
Yao
Yu-jia
YJ
Zhong
Lin
L
D000818:Animals; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism*; D009185:Myelin Proteins / Q000378:metabolism*; D000070798:Nogo Proteins; D009836:Oligodendroglia / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603197:Rtn4 protein, rat; D005947:Glucose; D010100:Oxygen