[Influence of iNOS silencing by RNA interference on proliferation activity of Tca8113 cell].
OBJECTIVE
To investigate the apoptosis and proliferation activity of Tca8113 cells.


METHODS
The vector that involves short hairpin RNA of iNOS was transfected to Tca8113 cells. The change of iNOS expression was observed using immunohistochemistry technique, the apoptosis rate examined by flow cytometry, and the proliferation Tca8113 cells examined by methyl thiazolyl tetrazolium (MTT).


RESULTS
The expression of iNOS in Psilencer-iNOS group was lower than that in control groups (P < 0.01), the apoptosis rate was higher than that in control groups (P < 0.01); whereas the proliferation activity of Tca8113 cells in Psilencer-iNOS group was lower than that in control groups.


CONCLUSIONS
Down expression of iNOS by RNAi can promotes apoptosis of Tca8113 cells and has an anti-proliferation activity effect.
Yang
Lan
L
Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510140, China.
Chen
Wei-Liang
WL
Zeng
Shu-Guang
SG
Zhang
Shu-Biao
SB
Shao
Jing
J
D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D006801:Humans; D052247:Nitric Oxide Synthase Type II / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D034622:RNA Interference; D014062:Tongue Neoplasms / Q000235:genetics / Q000473:pathology*; D014162:Transfection
C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II