Evaluating coupling procedures for synthesis of lna-Peptide conjugates.
As initial studies of block coupling of peptides with C-terminal glycines, we have evaluated of coupling of glycine building blocks under various conditions to a preassembled solid support bound LNA-fragment. Potentially competing side reaction have been studied and we have worked out a procedure for trifluoroacetyl protection of peptides.
Steunenberg
Peter
P
Department of Biosciences and Nutrition, Karolinska Institutet, S-14157 Huddinge, Sweden.
Leijonmarck
Hans
H
Svahn
Mathias G
MG
Smith
C I Edvard
CI
Strömberg
Roger
R
D001483:Base Sequence; D005998:Glycine / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011392:Proline / Q000737:chemistry
D009841:Oligonucleotides; D010455:Peptides; C477371:locked nucleic acid; D011392:Proline; D005998:Glycine