title: Striking alteration of some populations of T/B cells in systemic lupus erythematosus: relationship to expression of CD62L or some chemokine receptors.
abstract: Recent studies have revealed new populations of T/B cells, including central/effector memory, follicular T cells and CXCR3+ or CXCR4+ B cells. In the present study, changes in these populations of CD4+ T cells were examined on the basis of the expression of CD62L, CCR7 and CXCR5 in patients with systemic lupus erythematosus (SLE) in relation to CCL21 and CXCL10. Changes in CXCR3+, CXCR4+ and CXCR5+ B cells were also examined. CD62L and various chemokine receptors were examined by flow cytometry analysis using monoclonal antibodies, and CCL21 and CXCL10 were examined by sandwich enzyme-linked immunosorbent assay. In patients with SLE, a decrease of naive T cells and an increase in the ratio of activated effector memory T cells were associated with an increase of CCL21 and CXCL10 in serum, although the correlation was not significant. An increase in the ratio of CXCR3+ B cells was also recognized. These results suggest that naive T cells are transferred to lymphoid tissue by CCL21, and that effector memory T cells are activated by CXCL10. It is also suggested that B cells responsive to follicular helper T cells tend to migrate to inflammatory tissue.
authors: Department of Rheumatology and Internal Medicine, Juntendo University School of Medicine, Tokyo, Japan. ps-mind@wg7.so-net.ne.jp
authors: T
authors: T
authors: Watanabe
authors: J
authors: J
authors: Suzuki
authors: A
authors: A
authors: Mitsuo
authors: S
authors: S
authors: Nakano
authors: Y
authors: Y
authors: Tamayama
authors: A
authors: A
authors: Katagiri
authors: H
authors: H
authors: Amano
authors: S
authors: S
authors: Morimoto
authors: Y
authors: Y
authors: Tokano
authors: Y
authors: Y
authors: Takasaki
mesh_terms: D000328:Adult; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D054421:Chemokine CCL21 / Q000097:blood; D054357:Chemokine CXCL10 / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D019041:L-Selectin / Q000032:analysis*; D017493:Leukocyte Common Antigens / Q000032:analysis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008875:Middle Aged; D054400:Receptors, CCR7 / Q000032:analysis; D054367:Receptors, CXCR3 / Q000032:analysis; D019718:Receptors, CXCR4 / Q000032:analysis; D054380:Receptors, CXCR5 / Q000032:analysis; D019707:Receptors, Chemokine / Q000032:analysis*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: C515478:CCL21 protein, human; C515370:CCR7 protein, human; C114753:CXCL10 protein, human; C515187:CXCR3 protein, human; C527276:CXCR4 protein, human; C095085:CXCR5 protein, human; D054421:Chemokine CCL21; D054357:Chemokine CXCL10; D054400:Receptors, CCR7; D054367:Receptors, CXCR3; D019718:Receptors, CXCR4; D054380:Receptors, CXCR5; D019707:Receptors, Chemokine; D019041:L-Selectin; D017493:Leukocyte Common Antigens