Solid-supported synthesis of 5'-mRNA CAP-4 from Trypanosomatids.
The unique structure of 5' mRNA cap from Trypanosomatids is the most modified cap found in nature. Here we present the synthesis of cap-4 (m(7)Gpppm(3)(6,6,2')Apm(2')Apm(2')Cpm(2)(3,2')Up) on a disulfide-tethered solid support. This approach allows obtaining cap-4 more efficiently then previously described. Moreover such modified resin could be a useful tool for affinity purification of Leishmania proteins interacting with cap-4. For the final step of synthesis, namely coupling of phosphorylated tetranucleotide with activated 7-methylguanosine 5'-diphosphate two systems were compared. Surprisingly, the coupling in water with Mn(2+) as a catalyst, gave better results than usually more effective coupling in DMF with ZnCl(2).
Lewdorowicz
Magdalena
M
Department of Biophysics, Institute of Experimental Physics, Warsaw University, Zwirki i Wigury 93, Warsaw 02-089, Poland.
Jemielity
Jacek
J
Kierzek
Ryszard
R
Shapira
Michal
M
Stepinski
Janusz
J
Darzynkiewicz
Edward
E
D000818:Animals; D007891:Leishmania / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000737:chemistry / Q000302:isolation & purification; D012315:RNA Caps / Q000138:chemical synthesis* / Q000737:chemistry; D012333:RNA, Messenger / Q000138:chemical synthesis* / Q000737:chemistry; D016053:RNA, Protozoan / Q000138:chemical synthesis* / Q000737:chemistry
D015800:Protozoan Proteins; D012315:RNA Caps; D012333:RNA, Messenger; D016053:RNA, Protozoan