title: A combination in-ovo vaccine for avian influenza virus and Newcastle disease virus.
abstract: The protection of poultry from H5N1 highly pathogenic avian influenza A (HPAI) and Newcastle disease virus (NDV) can be achieved through vaccination, as part of a broader disease control strategy. We have previously generated a recombinant influenza virus expressing, (i) an H5 hemagglutinin protein, modified by the removal of the polybasic cleavage peptide and (ii) the ectodomain of the NDV hemagglutinin-neuraminidase (HN) protein in the place of the ectodomain of influenza neuraminidase (Park MS, et al. Proc Natl Acad Sci USA 2006;103(21):8203-8). Here we show this virus is attenuated in primary normal human bronchial epithelial (NHBE) cell culture, and demonstrate protection of C57BL/6 mice from lethal challenge with an H5 HA-containing influenza virus through immunisation with the recombinant virus. In addition, in-ovo vaccination of 18-day-old embryonated chicken eggs provided 90% and 80% protection against highly stringent lethal challenge by NDV and H5N1 virus, respectively. We propose that this virus has potential as a safe in-ovo live, attenuated, bivalent avian influenza and Newcastle disease virus vaccine.
authors: Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029-6574, USA.
authors: John
authors: J
authors: Steel
authors: Svetlana V
authors: SV
authors: Burmakina
authors: Colleen
authors: C
authors: Thomas
authors: Erica
authors: E
authors: Spackman
authors: Adolfo
authors: A
authors: García-Sastre
authors: David E
authors: DE
authors: Swayne
authors: Peter
authors: P
authors: Palese
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D053125:Influenza A Virus, H5N2 Subtype / Q000276:immunology; D007252:Influenza Vaccines / Q000008:administration & dosage; D005585:Influenza in Birds / Q000517:prevention & control*; D051379:Mice; D008810:Mice, Inbred C57BL; D009439:Neuraminidase / Q000235:genetics / Q000276:immunology; D009521:Newcastle Disease / Q000276:immunology / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000276:immunology*; D009883:Ophthalmic Solutions; D010063:Ovum / Q000276:immunology; D014611:Vaccination / Q000662:veterinary; D014613:Vaccines, Attenuated / Q000008:administration & dosage; D017778:Vaccines, Combined / Q000008:administration & dosage; D014765:Viral Vaccines / Q000008:administration & dosage*
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D009883:Ophthalmic Solutions; D014613:Vaccines, Attenuated; D017778:Vaccines, Combined; D014765:Viral Vaccines; C109963:hemagglutinin, avian influenza A virus; D009439:Neuraminidase