Selection of hematopoietic stem cells with a combination of galactose-bound vinyl polymer and soybean agglutinin, a galactose-specific lectin.
BACKGROUND
Selection of hematopoietic stem cells can be used to prevent graft-versus-host disease (GVHD) after allograft transplantation. The purpose of the study was to examine a novel cell separation system comprising a galactose-bound vinyl polymer (Gal-VP) and soybean agglutinin (SBA), a galactose-specific lectin.


METHODS
A vinyl polymer (VP) containing alpha-1,6- and beta-1,4-linked galactose terminals was used to facilitate cell separation. A VP containing an alpha-1,4-linked glucose terminal (alpha-1,4-Glu-VP) was also synthesized as a control for alpha-1,6- and beta-1,4-Gal-VP. Peripheral blood samples were collected from healthy volunteers and umbilical cord blood cells were collected after normal labor.


RESULTS
The sugar-VP was adsorbed on the surface of various materials. In the presence of SBA, T lymphocytes bound to beta-1,4-Gal-VP-coated microbeads, but not to alpha-1,4-Glu-VP-coated microbeads. When peripheral or cord blood cells were cultured on alpha-1,6-Gal-VP-coated plates, most red blood cells, lymphocytes, granulocytes, and monocytes adhered to the plate in the presence of 300 mg per mL SBA, whereas few CD34+ cells attached, even with 800 mg per mL SBA.


CONCLUSIONS
SBA binds selectively to blood cells by recognizing cell-surface sugars, which are dependent on the extent of cellular differentiation. Therefore, the combination of alpha-1,6-Gal-VP and SBA might be useful for separation of blood cells according to their stage of differentiation and lineage.
Yura
Hirofumi
H
NeTech, Inc., Kawasaki, Kanagawa, Japan.
Kanatani
Yasuhiro
Y
Ishihara
Masayuki
M
Takase
Bonpei
B
Nambu
Masaki
M
Kishimoto
Satoko
S
Kitagawa
Michihiro
M
Tatsuzawa
Osamu
O
Hoshi
Yasutaka
Y
Suzuki
Shinya
S
Kawakami
Mitsuyuki
M
Matsui
Takemi
T
D002469:Cell Separation / Q000379:methods*; D005690:Galactose / Q000737:chemistry*; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology*; D008954:Models, Biological; D008956:Models, Chemical; D015394:Molecular Structure; D037121:Plant Lectins / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D030262:Soybean Proteins / Q000737:chemistry*; D014753:Vinyl Compounds / Q000737:chemistry; D010835:Phytohemagglutinins
D005690:Galactose; D037121:Plant Lectins; D011108:Polymers; D030262:Soybean Proteins; D014753:Vinyl Compounds; C028824:soybean lectin; D010835:Phytohemagglutinins