Histological analysis of induced cartilage on the biodegradable or nonbiodegradable membranes from immature muscular tissue in vitro.
Successful tissue engineering relies on a combination of cells, cytokines, and appropriate scaffolds. Here, we tried to induce the formation of cartilage in vitro using immature muscular tissue, crude bone morphogenetic protein (BMP) as a source of the cytokine, and biodegradable membranes (BioMend; BM and GC-membrane; GC-M) or a nonbiodegradable membrane (GORE-TEX; GT) as scaffolds. Crude BMP was extracted from bovine cortical bones, dried, and dissolved in 1M urea before it was added to immature muscular tissue from the forelimbs of fetal Sprague Dawley rats at 20 days of pregnancy. The tissue was then cultured for 2 weeks in a carbon dioxide incubator. Complete cartilage was observed only when GT was used as a scaffold. In addition, cartilage-like tissue formation was observed when BM was used, and partial cartilage formation was observed for GC-M. Therefore, these results show that immature muscular tissue differentiated into cartilage and GORE-TEX is the most effective material for use as a scaffold in this model of tissue engineering.
Hayashi
Tatsuhide
T
Department of Dental and Material Science, Aichi-Gakuin University School of Dentistry, 1-100 Kusumoto-cho Chikusa-ku Nagoya 4648650, Japan. thayashi@dpc.agu.ac.jp
Kawai
Tatsushi
T
Ishikawa
Atsuko
A
Kawai
Hideki
H
Nakano
Kenjiro
K
Takei
Yukiko
Y
Kuroki
Kenjiro
K
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology; D002356:Cartilage / Q000166:cytology* / Q000187:drug effects; D002417:Cattle; D007150:Immunohistochemistry; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D009132:Muscles / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties
D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D008567:Membranes, Artificial