Two biosensors for phenolic compounds based on mushroom (Agaricus bisporus) homogenate: comparison in terms of some important parameters of the biosensors.
Interest in molecular imprinted polymer techniques has increased because they allows for the improvement of some stability characteristics of enzymes. The high stability of molecularly imprinted enzymes for a substrate can make them ideal alternatives as recognition elements for sensors. A bioimprinted mushroom tissue homogenate biosensor was constructed in a very simple way. For this purpose, sulfite was used. The enzyme, polyphenol oxidase, was first complexed by using a competitive inhibitor, sulfite, in aqueous medium and then the enzyme was immobilized on gelatin by crosslinking with glutaraldehyde on a glass electrode surface. Similarly, polyphenol oxidase uncomplexed with sulfite was also immobilized on a glass electrode in the same conditions. The aim of the study was to compare the two biosensors in terms of their repeatability and thermal, pH, and operational stability; also, the linear ranges of the two biosensors were compared with each other.
Teke
Mustafa
M
Faculty of Science and Arts, Chemistry Department, Muğla University, Muğla, Türkiye.
Sezgintürk
M Kemal
MK
Dinçkaya
Erhan
E
Telefoncu
Azmi
A
D000364:Agaricus / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004156:Catechol Oxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D002396:Catechols / Q000032:analysis; D003432:Cross-Linking Reagents / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004800:Enzymes, Immobilized; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D005780:Gelatin / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013447:Sulfites / Q000494:pharmacology; D013696:Temperature; D014867:Water / Q000737:chemistry
D002396:Catechols; D003432:Cross-Linking Reagents; D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized; D010636:Phenols; D013447:Sulfites; D014867:Water; D005780:Gelatin; D004156:Catechol Oxidase; D005976:Glutaral