Study of mean absorptive potential using Lenz model: toward quantification of phase contrast from an electrostatic phase plate.
An electrostatic phase plate can provide better phase contrast, a fact that plays a promising role for the high-resolution observation of specimens containing light elements. However, in order to quantify the "phase" contrast from images recorded using the phase plate, the "absorption" (or scattering) contrast arising from electrons scattered elastically and inelastically outside of the phase-plate ring must be analyzed. Angular distributions of the elastic and inelastic scattering are predicted using the Lenz model. The mean absorptive potential, V(o) serving as an index for the contribution of "absorption" contrast, is calculated from the reciprocal mean free path of elastic and inelastic scattering, and is verified experimentally. The mean absorptive potential of a particular phase plate with inner and outer radii of 0.25microm (theta(1)=0.09mrad) and 1mum (theta(2)=0.4mrad), respectively, is approximately 0.11eV for carbon and is equivalent to that of an objective aperture of semiangle 17mrad (cutoff frequency 6.7nm(-1)).
Chen
Ko-Feng
KF
Department of Engineering and System Science, National Tsing Hua University, HsinChu 30013, Taiwan.
Chang
Chia-Seng
CS
Shiue
Jessie
J
Hwu
Yeukuang
Y
Chang
Wei-Hau
WH
Kai
Ji-Jung
JJ
Chen
Fu-Rong
FR
D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000379:methods*; D008858:Microscopy, Phase-Contrast; D008962:Models, Theoretical; D012542:Scattering, Radiation