Mismatch base pair detection by fluorescence spectral change upon addition of metal cation--toward efficient analysis of single nucleotide polymorphism.
Addition of mercury (II) cation to fluorescent-labeled duplex involving a T:T mismatch base pair and silver (I) cation to fluorescent-labeled duplex involving a C:C mismatch base pair significantly changed the fluorescence intensity, but no significant change in the fluorescence intensity was observed for duplexes involving the other base pairs. The fluorescence spectral change upon addition of the metal cation can discriminate T:T and C:C mismatch base pairs from the other base pairs. Our results certainly support the idea that the fluorescence spectral change upon addition of the metal cation could be a convenient strategy for the mismatch base pair detection by the heteroduplex analysis, and may eventually lead to progress in single nucleotide polymorphism genotyping.
Torigoe
Hidetaka
H
Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan. htorigoe@ch.kagu.tus.ac.jp
Ono
Akira
A
Kozasa
Tetsuo
T
D020137:Base Pair Mismatch; D002413:Cations, Divalent; D003596:Cytosine / Q000737:chemistry; D004252:DNA Mutational Analysis; D008628:Mercury / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013941:Thymine / Q000737:chemistry
D002413:Cations, Divalent; D003596:Cytosine; D008628:Mercury; D013941:Thymine