[Preventive effects of 4 Se-enriched plants on rat stomach cancer induced by MNNG--1. inhibitary effects of different selenium resources on rat aneuploid cell incidence in mucosal epithelium of gastric antrum].
OBJECTIVE
To obtain new Se resources with high healthy value (both high activity and low toxicity), the preventive efficacies of three Se-enriched higher plants on stomach cancer were compared with selenite and Se-enriched garlic.


METHODS
Ninety weanling male Wistar rats were fed the basal diet for a week, divided equally into nine groups, control, MNNG,Se 75 and 150 microg/kg bw of selenite, Se 150 and 300 microg/kg bw of Se-enriched garlic, Se 150 microg/kg bw of Se-enriched broccoli, Se 300 microg/kg bw of Se-enriched red kales and green kales group. Rats in MNNG and Se supplementation groups were daily given 15 mg/kg bw of MNNG (solved in 1 ml distilled water) and the rats of control group were given 1 ml distilled water by gavage for ten days. Meanwhile, the rats of the control and MNNG group were daily given 1 ml distilled water and the rats of other groups were given 1 ml water suspension of Se-enriched garlic, red kavas, green kavas, broccoli or 1 ml solution of sodium selenite by gavage for seventeen weeks. All rats were freely fed the basal diet and water during the period of the experiment. At the end of 18th week, the rats were sacrificed, the incidence of aneuploid cells (IAC) in mucosal epithelium of the gastric antrum was detected, and the IAC data were analyzed by SPSS 12.0.


RESULTS
The results showed that the IACs were 25% and 30%, respectively, in the Se 150 microg/kg bw of Se-enriched garlic and -broccoli group, and were in turn 22%, 50% and 30% in the 300 microg/kg bw of Se-enriched garlic, -red kale and -green kale. No significant differences of IACs were found in the same level of Se supplementation groups by Se-enriched plants.


CONCLUSIONS
The data showed that Se-enriched broccoli, red kale and green kale had high activities similar to Se-enriched garlic in stomach cancer prevention and lower toxicity than selenite.
Yang
Wenjie
W
Institute of Nutrition and Food Safety, Chinese Center for Disease Prevention and Control, Beijing 100050, China.
Li
Weidong
W
Chen
Jing
J
Chen
Xiaobin
X
D000782:Aneuploidy; D000818:Animals; D001937:Brassica / Q000737:chemistry*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology; D005737:Garlic / Q000737:chemistry*; D005753:Gastric Mucosa / Q000166:cytology*; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000633:toxicity*; D011706:Pyloric Antrum / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000302:isolation & purification / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000517:prevention & control
D008769:Methylnitronitrosoguanidine; D012643:Selenium