title: An optimal embryo transfer condition for the effective production of DBA/2J mice.
abstract: The DBA/2J mouse strain is a standard laboratory strain that is widely used for biomedical research. This strain, however, suffers from poor reproductive performance. In addition, the conditions for reliable embryo transfer (ET) of this strain have not been elucidated. The intention of this study was to determine the optimal number of embryos for transfer that allow the effective production of DBA/2J offspring. In the experiment, 7 to 15 embryos per oviduct were transferred into pseudopregnant ICR females. A relatively high success rate for pup production was observed when a large number of DBA/2J embryos (30 embryos per female) were transferred. This result shows that the ET efficiency of the DBA/2J strain can be improved by increasing the number of transferred embryos.
authors: Functional Genomics Research Group, RIKEN Genomic Sciences Center, Ibaraki, Japan.
authors: Hideki
authors: H
authors: Kaneda
authors: Kyuichi
authors: K
authors: Taguma
authors: Chigusa
authors: C
authors: Suzuki
authors: Ai
authors: A
authors: Ozaki
authors: Chika
authors: C
authors: Nakamura
authors: Akiko
authors: A
authors: Hachisu
authors: Kimio
authors: K
authors: Kobayashi
authors: Shigeharu
authors: S
authors: Wakana
authors: Toshihiko
authors: T
authors: Shiroishi
mesh_terms: D000818:Animals; D004624:Embryo Transfer / Q000379:methods / Q000662:veterinary*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA / Q000502:physiology*; D008813:Mice, Inbred ICR