Candida rugosa lipase-catalysed kinetic resolution of 2-substituted-aryloxyacetic esters with dimethylsulfoxide and isopropanol as additives.
Candida rugosa lipase-catalysed hydrolysis of three different 2-substituted-aryloxyacetic esters was performed in aqueous buffer containing dimethyl sulphoxide and isopropanol from 0 to 80% v/v as additives, in order to obtain an enhancement of the enantioselectivity. For 2-(p-chlorophenoxy)acetic acid and 2-n-butyl-2-(p-chlorophenoxy)acetic acid ethyl esters, DMSO enhanced enzyme enantioselectivity more than IPA with an opposite enzymatic enantiopreference. The cosolvents moderately improved Candida rugosa lipase enantioselectivity for 2-phenyl-2-(p-chlorophenoxy)acetic acid ethyl ester.
Ammazzalorso
Alessandra
A
Department of Drug Sciences, University of Chieti G. d'Annunzio, via dei Vestini, Chieti, Italy.
Amoroso
Rosa
R
Bettoni
Giancarlo
G
De Filippis
Barbara
B
Fantacuzzi
Marialuigia
M
Giampietro
Letizia
L
Maccallini
Cristina
C
Tricca
Maria Luisa
ML
D019840:2-Propanol / Q000494:pharmacology*; D002175:Candida / Q000201:enzymology*; D002384:Catalysis / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004952:Esters / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D013237:Stereoisomerism
D004952:Esters; D008049:Lipase; D019840:2-Propanol; D004121:Dimethyl Sulfoxide