The role of hemocytes in the immunity of the spider Acanthoscurria gomesiana.
Invertebrates protect themselves against microbial infection through cellular and humoral immune defenses. Since the available information on the immune system of spiders is scarce, the main goal of the present study was to investigate the role of hemocytes and antimicrobial peptides (AMPs) in defense against microbes of spider Acanthoscurria gomesiana. We previously described the purification and characterization of two AMPs from the hemocytes of naïve spider A. gomesiana, gomesin and acanthoscurrin. Here we show that 57% of the hemocytes store both gomesin and acanthoscurrin, either in the same or in different granules. Progomesin labeling in hemocyte granules indicates that gomesin is addressed to those organelles as a propeptide. In vivo and in vitro experiments showed that lipopolysaccharide (LPS) and yeast caused the hemocytes to migrate. Once they have reached the infection site, hemocytes may secrete coagulation cascade components and AMPs to cell-free hemolymph. Furthermore, our results suggest that phagocytosis is not the major defense mechanism activated after microbial challenge. Therefore, the main reactions involved in the spider immune defense might be coagulation and AMP secretion.
Fukuzawa
Aline H
AH
Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.
Vellutini
Bruno C
BC
Lorenzini
Daniel M
DM
Silva
Pedro I
PI
Mortara
Renato A
RA
da Silva
José M C
JM
Daffre
Sirlei
S
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000276:immunology* / Q000378:metabolism; D001779:Blood Coagulation Factors / Q000276:immunology / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology; D020869:Gene Expression Profiling; D006434:Hemocytes / Q000276:immunology* / Q000382:microbiology / Q000648:ultrastructure; D007109:Immunity; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000276:immunology* / Q000648:ultrastructure; D008070:Lipopolysaccharides / Q000494:pharmacology; D018613:Microscopy, Confocal; D009181:Mycoses / Q000276:immunology; D010587:Phagocytosis / Q000276:immunology; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D012441:Saccharomyces cerevisiae; D013112:Spiders / Q000276:immunology*
D023181:Antimicrobial Cationic Peptides; D001779:Blood Coagulation Factors; D019476:Insect Proteins; D008070:Lipopolysaccharides; C483971:acanthoscurrin 1 protein, Acanthoscurria gomesiana; C415987:gomesin