title: Trichlorfon effects on mouse oocytes following in vivo exposure.
abstract: Trichlorfon (TCF) is a widely used pesticide, which according to some epidemiological and experimental data, is suspected of being aneugenic in human and mouse cells. In particular, in vitro studies in mouse oocytes showed the induction of aneuploidy and polyploidy at the first meiotic division and of severe morphological alterations of the second meiotic spindle. We have tested the hypothesis that an acute treatment of mice with TCF might similarly affect chromosome segregation in maturing oocytes. Superovulated MF-1 mice were intraperitoneally injected with 400mg/kg TCF or orally administered with 600mg/kg TCF either at the time of or 4h after human chorionic gonadotrophin (HCG) injection. Oocytes were harvested 17h after HCG and metaphase II chromosomes were cytogenetically analyzed. No significant increase of aneuploid or polyploid cells was detected at any treatment condition. A significant (p<0.001) decrease of metaphases showing premature chromatid separation or premature anaphase II in all TCF-treated groups with respect to controls suggested that TCF treatment may have delayed the first meiotic division. To evaluate possible effects of the pesticide upon the second meiotic division, a group of females orally treated with 600mg/kg TCF at resumption of meiosis was mated with untreated males and zygotes were collected for cytogenetic analysis. No evidence of aneuploidy induction was obtained, but the frequency of polyploid zygotes was increased fivefold over the control level (p<0.01). Such polyploid embryos might have arisen from fertilization of oocytes that were either meiotically delayed and still in metaphase I at fertilization or progressed through anaphase II without cytokinesis. These findings show that in vivo studies on aneuploidy induction in oocytes may yield results different from those obtained by in vitro experiments and that both kinds of data may be necessary for risk assessment of environmentally relevant exposures.
authors: Section of Toxicology and Biomedical Sciences, ENEA CR Casaccia, Via Anguillarese 301, I-00123 Rome, Italy.
authors: Roberto
authors: R
authors: Ranaldi
authors: Gloria
authors: G
authors: Gambuti
authors: Ursula
authors: U
authors: Eichenlaub-Ritter
authors: Francesca
authors: F
authors: Pacchierotti
mesh_terms: D024422:Aneugens / Q000008:administration & dosage / Q000633:toxicity*; D000782:Aneuploidy; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D007274:Injections, Intraperitoneal; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011123:Polyploidy; D014236:Trichlorfon / Q000008:administration & dosage / Q000633:toxicity*; D015053:Zygote / Q000187:drug effects / Q000378:metabolism
chemical_list: D024422:Aneugens; D014236:Trichlorfon