title: Antiradical capacity and induction of apoptosis on HeLa cells by a Phaseolus vulgaris extract.
abstract: Jamapa bean is a black Phaseolus vulgaris variety rich in condensed tannins, anthocyanins and flavonols with interesting biological activities. The objective of this work was to evaluate the antiradical capacity (ARC) of a Jamapa bean methanolic extract (BME) and some of the proanthocyanidin-rich fractions derived from it, using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The effect of the BME on some proteins involved in apoptosis on HeLa cells was also evaluated. A strong correlation between proanthocyanidin concentration in BME and antiradical capacity was found, suggesting that these compounds contribute significantly to antiradical activity. BME was a better radical scavenger than butylated hydroxytoluene (45.6 and 33.9% ARC at 400 microM, respectively). Two proanthocyanidin-rich fractions obtained after a preliminary separation of the BME using Toyopearl (TP4 and TP6) exhibited a higher antiradical activity than the parent extract. The treatment of HeLa cells with 35 microg BME/ml/24 h increased the expression of Bax and Caspase-3, pro-apoptotic proteins (6.13 and 1.2 times for Caspase-3 and Bax, respectively). The mechanism of action of some proteins involved in apoptosis was also evaluated, and the results suggest that black Jamapa bean could be an important source of polyphenolic compounds with potential biological use as antioxidant and anticancer agents.
authors: Programa de Posgrado en Alimentos del Centro de la Republica (PROPAC), Research and Graduate Studies in Food Science, School of Chemistry, Universidad Autonoma de Queretaro, Queretaro, Qro., 76010, Mexico.
authors: Xochitl
authors: X
authors: Aparicio-Fernández
authors: Rosalia
authors: R
authors: Reynoso-Camacho
authors: Eduardo
authors: E
authors: Castaño-Tostado
authors: Teresa
authors: T
authors: García-Gasca
authors: Elvira
authors: E
authors: González de Mejía
authors: S Horacio
authors: SH
authors: Guzmán-Maldonado
authors: Guillermo
authors: G
authors: Elizondo
authors: Gad Gabra
authors: GG
authors: Yousef
authors: Mary Ann
authors: MA
authors: Lila
authors: Guadalupe
authors: G
authors: Loarca-Pina
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001713:Biphenyl Compounds; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers; D006367:HeLa Cells; D006801:Humans; D006834:Hydrazines; D027805:Phaseolus / Q000737:chemistry*; D010851:Picrates; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000032:analysis* / Q000494:pharmacology*
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006834:Hydrazines; D010851:Picrates; D010936:Plant Extracts; D044945:Proanthocyanidins; C013221:proanthocyanidin; C004931:1,1-diphenyl-2-picrylhydrazyl