title: Metabotyping of Caenorhabditis elegans reveals latent phenotypes.
abstract: Assigning functions to every gene in a living organism is the next challenge for functional genomics. In fact, 85-90% of the 19,000 genes of the nematode Caenorhabditis elegans genome do not produce any visible phenotype when inactivated, which hampers determining their function, especially when they do not belong to previously characterized gene families. We used (1)H high-resolution magic angle spinning NMR spectroscopy ((1)H HRMAS-NMR) to reveal the latent phenotype associated to superoxide dismutase (sod-1) and catalase (ctl-1) C. elegans mutations, both involved in the elimination of radical oxidative species. These two silent mutations are significantly discriminated from the wild-type strain and from each other. We identify a metabotype significantly associated with these mutations involving a general reduction of fatty acyl resonances from triglycerides, unsaturated lipids being known targets of free radicals. This work opens up perspectives for the use of (1)H HRMAS-NMR as a molecular phenotyping device for model organisms. Because it is amenable to high throughput and is shown to be highly informative, this approach may rapidly lead to a functional and integrated metabonomic mapping of the C. elegans genome at the systems biology level.
authors: Centre de Resonance Magnétique Nucléaire à Très Hauts Champs and Laboritoire de Chimie, Ecole Normale Supérieure de Lyon/Centre National de la Recherche Scientifique, Université de Lyon, 69364 Lyon, France.
authors: Benjamin J
authors: BJ
authors: Blaise
authors: Jean
authors: J
authors: Giacomotto
authors: Bénédicte
authors: B
authors: Elena
authors: Marc-Emmanuel
authors: ME
authors: Dumas
authors: Pierre
authors: P
authors: Toulhoat
authors: Laurent
authors: L
authors: Ségalat
authors: Lyndon
authors: L
authors: Emsley
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D049751:Genome, Helminth; D023281:Genomics; D009682:Magnetic Resonance Spectroscopy; D009154:Mutation; D018384:Oxidative Stress / Q000235:genetics; D010641:Phenotype; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1
chemical_list: D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1