Changes in aurantio-obtusin and glucoaurantio-obtusin content in Cassiae Semen via treatment with a crude enzyme extract from Aspergillus usamii.
Cassiae Semen (seeds of Cassia tora) showed a remarkably different HPLC chromatogram after being treated with a crude enzyme extract from Aspergillus usamii. Increased and decreased compounds were identified as aurantio-obtusin and glucoaurantio-obtusin, respectively. The aurantio-obtusin content reached its maximum level (133.58 +/- 0.39 microg/mg extract) after being incubated for 50 min at 37 degrees C, whereas the inactivated crude enzyme-treated control remained unchanged (54.13 +/- 1.33 microg/mg). On the other hand, the glucoaurantio-obtusin content decreased by less than one-third (51.09 +/- 1.63 microg/ mg) of the untreated control (143.19 +/- 2.12 microg/mg), suggesting that an increase in aurantio-obtusin content originated from the enzymatic cleavage of its glucoside glucoaurantio-obtusin.
Hur
Jong-Moon
JM
School of Applied Biosciences, College of Agriculture & Life Sciences, Kyungpook National University, Daegu 702-701, Korea.
Kwon
Soon-Ho
SH
So
Jae-Hyun
JH
Jun
Mira
M
Kang
Young-Hwa
YH
Lee
Yu-Mi
YM
Lee
Kyung-Bok
KB
Rhee
In-Koo
IK
Lee
Moon-Soon
MS
Song
Kyung-Sik
KS
D000880:Anthraquinones / Q000032:analysis*; D001230:Aspergillus / Q000201:enzymology*; D002366:Cassia / Q000737:chemistry*; D005285:Fermentation; D005511:Food Handling
D000880:Anthraquinones