title: Stability of the T-DNA flanking regions in transgenic Arabidopsis thaliana plants under influence of abiotic stress and cultivation practices.
abstract: Genetic transformation is often associated with different rearrangements of the plant genome at the site of insertion. Therefore the question remains weather these T-DNA insertion sites are more prone to genotoxic stresses. Here, we studied the impact of propagation through generations, the influence of gene stacking and of photo oxidative stress caused by high light intensity on the stability of the transgene flanking regions in the model plant Arabidopsis thaliana. Conformational Sensitive Capillary Electrophoresis (CSCE), RFLP and sequencing were deployed in this analysis in order to study the proximal 100 bp and the long-range T-DNA flanking sequences. By screening seven transgenic lines no evidence for occurrence of mutation events were found, implying that the nucleotide sequence of the T-DNA flanking regions of the studied events is unlikely to be unstable.
authors: Institute for Agricultural and Fisheries Research, Burg. Van Gansberghelaan 115, 9820, Merelbeke, Belgium. nina.papazova@ilvo.vlaanderen.be
authors: Nina
authors: N
authors: Papazova
authors: Rim
authors: R
authors: Ghedira
authors: Sabine
authors: S
authors: Van Glabeke
authors: Aghleb
authors: A
authors: Bartegi
authors: Pieter
authors: P
authors: Windels
authors: Isabel
authors: I
authors: Taverniers
authors: Isabel
authors: I
authors: Roldan-Ruiz
authors: Erik
authors: E
authors: Van Bockstaele
authors: Anne
authors: A
authors: Milcamps
authors: Guy
authors: G
authors: Van Den Eede
authors: Ann
authors: A
authors: Depicker
authors: Marc
authors: M
authors: De Loose
mesh_terms: D024509:3' Flanking Region; D024506:5' Flanking Region; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D004269:DNA, Bacterial / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D009154:Mutation; D030821:Plants, Genetically Modified
chemical_list: D004269:DNA, Bacterial; D018744:DNA, Plant; C036483:T-DNA