title: A non-tumor suppressor role for basal p19ARF in maintaining nucleolar structure and function.
abstract: The nucleolus is the center of ribosome synthesis, with the nucleophosmin (NPM) and p19(ARF) proteins antagonizing one another to either promote or inhibit growth. However, basal NPM and ARF proteins form nucleolar complexes whose functions remain unknown. Nucleoli from Arf(-/)(-) cells displayed increased nucleolar area, suggesting that basal ARF might regulate key nucleolar functions. Concordantly, ribosome biogenesis and protein synthesis were dramatically elevated in the absence of Arf, causing these cells to exhibit tremendous gains in protein amounts and increases in cell volume. The transcription of ribosomal DNA (rDNA), the processing of nascent rRNA molecules, and the nuclear export of ribosomes were all increased in the absence of ARF. Similar results were obtained using targeted lentiviral RNA interference of ARF in wild-type MEFs. Postmitotic osteoclasts from Arf-null mice exhibited hyperactivity in vitro and in vivo, demonstrating a physiological function for basal ARF. Moreover, the knockdown of NPM blocked the increases in Arf(-/-) ribosome output and osteoclast activity, demonstrating that these gains require NPM. Thus, basal ARF proteins act as a monitor of steady-state ribosome biogenesis and growth independent of their ability to prevent unwarranted hyperproliferation.
authors: Department of Internal Medicine, Division of Molecular Oncology, Washington University School of Medicine, Campus Box 8069, 660 S. Euclid Avenue, St. Louis, MO 63110, USA. jweber@im.wustl.edu
authors: Anthony J
authors: AJ
authors: Apicelli
authors: Leonard B
authors: LB
authors: Maggi
authors: Angela C
authors: AC
authors: Hirbe
authors: Alexander P
authors: AP
authors: Miceli
authors: Mary E
authors: ME
authors: Olanich
authors: Crystal L
authors: CL
authors: Schulte-Winkeler
authors: Anthony J
authors: AJ
authors: Saporita
authors: Michael
authors: M
authors: Kuchenreuther
authors: José
authors: J
authors: Sanchez
authors: Katherine
authors: K
authors: Weilbaecher
authors: Jason D
authors: JD
authors: Weber
mesh_terms: D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000502:physiology*; D004275:DNA, Ribosomal / Q000235:genetics; D051379:Mice; D009687:Nuclear Proteins / Q000502:physiology*; D000090243:Nucleophosmin; D014176:Protein Biosynthesis; D012335:RNA, Ribosomal / Q000235:genetics; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D025521:Tumor Suppressor Proteins
chemical_list: C417743:Cdkn2a protein, mouse; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004275:DNA, Ribosomal; D009687:Nuclear Proteins; D012335:RNA, Ribosomal; D025521:Tumor Suppressor Proteins; D000090243:Nucleophosmin