Expression of apoptotic and cell proliferation regulatory proteins in keratoacanthomas and squamous cell carcinomas of the skin.
The aim of this study was to investigate the expression of p53, caspase-3, bcl-2, MIB-1, and PCNA to validate more objective methods to differentiate squamous cell carcinoma and keratoacanthoma, as well as to understand their pathogenesis with accuracy. A total of 52 cases of histopathologically diagnosed keratoacanthoma in the proliferative stage and 56 cases of well-differentiated squamous cell carcinoma were selected in this study. The expression was evaluated by means of immunohistochemistry. Bcl-2 immunoreactivity was weak or absent in the majority of cases, either in squamous cell carcinoma or in keratoacanthoma. PCNA-positive cells did not show differences between two lesions evaluated. On the other hand, MIB-1 was statistically significant (p<0.05) between squamous cell carcinomas and keratoacanthomas. Moreover, p53 and caspase-3 were overexpressed in squamous cell carcinomas. Together, these results suggest that the biological behavior of the well-differentiated squamous cell carcinomas of the skin may be associated with cellular proliferation and/or deregulation of cell death, indicated by increased expression of the MIB-1 and apoptotic proteins p53 and caspase-3, respectively.
Ribeiro
Daniel
D
Department of Health Sciences, Federal University of Sao Paulo, UNIFESP, Santos, SP, Brazil.
Narikawa
Silvia
S
Marques
Mariângela E A
ME
D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D053148:Caspase 3 / Q000032:analysis; D018797:Cell Cycle Proteins / Q000032:analysis*; D002454:Cell Differentiation; D049109:Cell Proliferation; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D007636:Keratoacanthoma / Q000201:enzymology / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D019394:Ki-67 Antigen / Q000032:analysis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D012878:Skin Neoplasms / Q000737:chemistry* / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
D051017:Apoptosis Regulatory Proteins; D018797:Cell Cycle Proteins; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C505407:CASP3 protein, human; D053148:Caspase 3