title: Optimization of expression conditions for soluble protein by using a robotic system of multi-culture vessels.
abstract: We have developed a robotic system for an automated parallel cell cultivation process that enables screening of induction parameters for the soluble expression of recombinant protein. The system is designed for parallelized and simultaneous cultivation of up to 24 different types of cells or a single type of cell at 24 different conditions. Twenty-four culture vessels of about 200 ml are arranged in four columns x six rows. The system is equipped with four independent thermostated waterbaths, each of which accommodates six culture vessels. A two-channel liquid handler is attached in order to distribute medium from the reservoir to the culture vessels, to transfer seed or other reagents, and to take an aliquot from the growing cells. Cells in each vessel are agitated and aerated by sparging filtered air. We tested the system by growing Escherichia coli BL21(DE3) cells harboring a plasmid for a model protein, and used it in optimizing protein expression conditions by varying the induction temperature and the inducer concentration. The results revealed the usefulness of our custom-made cell cultivation robot in screening optimal conditions for the expression of soluble proteins.
authors: Life Sciences Division, Korea Institute of Science and Technology, Seoul 136-791, Korea.
authors: Woo-Sung
authors: WS
authors: Ahn
authors: Ji-Young
authors: JY
authors: Ahn
authors: Chan-Hun
authors: CH
authors: Jung
authors: Kwang Yeon
authors: KY
authors: Hwang
authors: Eunice Eunkyeong
authors: EE
authors: Kim
authors: Joon
authors: J
authors: Kim
authors: Hana
authors: H
authors: Im
authors: Jin-Oh
authors: JO
authors: Kim
authors: Myeong-Hee
authors: MH
authors: Yu
authors: Cheolju
authors: C
authors: Lee
mesh_terms: D049109:Cell Proliferation; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012371:Robotics; D013696:Temperature
chemical_list: D011994:Recombinant Proteins