title: Structural analysis of cell wall mannan of Candida sojae, a new yeast species isolated from defatted soybean flakes.
abstract: We investigated the structural and immunochemical characteristics of cell wall mannan obtained from Candida sojae JCM 1644, which is a new yeast species isolated from defatted soybean flakes. The results of a slide-agglutination test and of an enzyme-linked immunosorbent assay using anti-factor sera to the pathogenic Candida species indicated that the cells and the C. sojae mannan were cross-reactive to the specific anti-factor sera against Candida albicans serotype A (FAb 6) and Candida guilliermondii (FAb 9). Two-dimensional homonuclear Hartmann-Hahn analysis indicated that the mannan consisted of various linked oligomannosyl side chains containing alpha-1,2-, alpha-1,3-, alpha-1,6- and beta-1,2-linked mannose residues. However, although the determinants of antigenic factors 6 and 9 could be not found in this mannan, branched side chains, Manbeta1-2Manalpha1-3[Manalpha1-6]Manalpha1-(2Manalpha1-)n2Man and a linear alpha-1,6-linked polymannosyl backbone, which are cross-reacted by FAbs 6 and 9, respectively, were identified. The mannan was subjected to acetolysis in order to determine the polymerization length of the alpha-1,2-linked oligomannosyl residue in the side chains. The result of (1)H-nuclear magnetic resonance analysis of the released oligosaccharides showed that the remarkable regularity in the length of alpha-1,2-linked oligomannosyl side chains, which were previously found in mannans of other Candida species, is not observed in this mannan.
authors: Department of Microbiology, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki, Japan.
authors: Hiroko
authors: H
authors: Oyamada
authors: Yukiko
authors: Y
authors: Ogawa
authors: Nobuyuki
authors: N
authors: Shibata
authors: Yoshio
authors: Y
authors: Okawa
authors: Shigeo
authors: S
authors: Suzuki
authors: Hidemitsu
authors: H
authors: Kobayashi
mesh_terms: D002175:Candida / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D008351:Mannans / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D025942:Oligosaccharides, Branched-Chain / Q000737:chemistry; D013025:Glycine max / Q000382:microbiology
chemical_list: D008351:Mannans; D025942:Oligosaccharides, Branched-Chain