title: Analysis of microtubule-mediated intracellular viral transport.
abstract: Host microtubules and motor proteins are crucial to the intracellular transport of a number of viruses. Disruption of microtubules or suppression of motor functions can remarkably inhibit the movement of viruses in host cells. It is now known that incoming viruses use motor proteins to travel along microtubules from the plasma membrane to the nuclear or perinuclear replication site, whereas progeny viruses depend on microtubules and motors to move from the assembly site to the cell periphery. Here, we describe several major methods for analyzing microtubule-mediated intracellular viral transport, using adenovirus as an example.
authors: Nankai University, Tianjin, China.
authors: Chunyong
authors: C
authors: Liu
authors: Min
authors: M
authors: Liu
authors: Jun
authors: J
authors: Zhou
mesh_terms: D000256:Adenoviridae / Q000737:chemistry / Q000502:physiology*; D001692:Biological Transport; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology / Q000821:virology; D002478:Cells, Cultured; D000072159:Dynactin Complex; D006801:Humans; D042541:Intracellular Space / Q000502:physiology* / Q000821:virology*; D008856:Microscopy, Fluorescence / Q000379:methods; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis; D008870:Microtubules / Q000737:chemistry* / Q000502:physiology* / Q000821:virology; D020409:Molecular Motor Proteins / Q000737:chemistry / Q000502:physiology; D014966:Xanthenes / Q000737:chemistry
chemical_list: D000072159:Dynactin Complex; D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; D014966:Xanthenes; C034657:Texas red