title: Both senescence and apoptosis induced by deprivation of growth factors were inhibited by a novel butyrolactone derivative through depressing integrin beta4 in vascular endothelial cells.
abstract: Both senescence and apoptosis of vascular endothelial cells (VECs) are involved in the development of cardiovascular diseases, including atherosclerosis. To understand the association between senescence and apoptosis in vascular endothelial cells, the authors first explored whether senescence and apoptosis took place at the same time in human umbilical vein endothelial cells (HUVECs) deprived of the growth factors. Integrin beta4 is a key factor in HUVEC apoptosis, to know whether this integrin is implicated in VEC senescence, the authors checked the changes of integrin beta4 level during HUVEC aging. Then the authors investigated the effects of 3BDO (3-benzyl-5-((2-nitrophenoxy)methyl)-dihydrofuran-2(3H)-one) on the senescence induced by deprivation of serum and fibroblast growth factor (FGF)-2. The results showed that deprivation of growth factors not only induced apoptosis, but also triggered senescence in HUVECs. The authors found that the level of integrin beta 4 was increased markedly during HUVEC senescence. 3BDO (20 to 60 microg/mL) could inhibit both senescence and apoptosis and depress integrin beta 4 level. The data suggested that integrin beta4 might be a pivotal factor in the relationship between senescence and apoptosis.
authors: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan, PR China.
authors: Weiwei
authors: W
authors: Wang
authors: Xia
authors: X
authors: Liu
authors: Yun
authors: Y
authors: Zhang
authors: Jing
authors: J
authors: Zhao
authors: Baoxiang
authors: B
authors: Zhao
authors: Shangli
authors: S
authors: Zhang
authors: Junying
authors: J
authors: Miao
mesh_terms: D017209:Apoptosis; D002478:Cells, Cultured; D016922:Cellular Senescence; D053938:DNA Fragmentation / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000172:deficiency; D005663:Furans / Q000494:pharmacology*; D006801:Humans; D039663:Integrin beta4 / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000172:deficiency*; D044967:Serum; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D005663:Furans; D039663:Integrin beta4; D036341:Intercellular Signaling Peptides and Proteins; D016222:Fibroblast Growth Factor 2; D001616:beta-Galactosidase