title: Biophysical properties of quadruplexes containing two or three 8-bromodeoxyguanosine residues.
abstract: A physico-chemical characterization, based on NMR and CD spectroscopy, of quadruplexes formed by the oligonucleotide d(TGGGT), where two or three Gs are substituted by 8-bromo-2'-deoxyguanosine residues (dGBr), is reported. The oligonucleotidic sequences d(TGBr GBr GT), d(TGBr GGBr T), d(TGGBr GBr T), and d(TGBr GBr GBr T) have been synthesized. Only sequences d(TGBr GGBr T) and d(TGBr GBr GT) were able to fold into a well defined quadruplex structure, and their CD profiles and thermal stabilities turned out to be very different from those observed for the natural counterpart, indicating that the 8-Br-dG residues dramatically affect the structure of the quadruplex.
authors: Dipartimento di Chimica, Università degli Studi di Napoli Federico II, Napoli, Italy.
authors: Luigi
authors: L
authors: Petraccone
authors: Ida
authors: I
authors: Duro
authors: Antonio
authors: A
authors: Randazzo
authors: Ada
authors: A
authors: Virno
authors: Luciano
authors: L
authors: Mayol
authors: Concetta
authors: C
authors: Giancola
mesh_terms: D002942:Circular Dichroism; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry; D054856:G-Quadruplexes; D009682:Magnetic Resonance Spectroscopy; D009691:Nucleic Acid Denaturation; D011522:Protons; D013816:Thermodynamics
chemical_list: D011522:Protons; D003849:Deoxyguanosine