title: Chlamydia pneumoniae infection increases adherence of mouse macrophages to mouse endothelial cells in vitro and to aortas ex vivo.
abstract: Interactions between monocytes/macrophages and endothelial cells play an important role in the pathogenesis of atherosclerosis, and the adherence of monocytes to the arterial endothelium is one of the early events in atherogenesis. In the present study, peritoneal macrophages harvested from green fluorescent protein (GFP) transgenic mice were used to analyze how Chlamydia pneumoniae infection affects the adherence of GFP-macrophages to mouse endothelial cells in vitro and to the aorta from normolipidemic and hyperlipidemic mice ex vivo. In vitro studies showed that C. pneumoniae-infected GFP-macrophages adhered better than uninfected macrophages to endothelial cells and GFP-macrophages adhered better to infected than uninfected endothelial cells. The ex vivo studies showed that C. pneumoniae-infected macrophages adhered better than uninfected macrophages to aortas from both normolipidemic and hyperlipidemic C57BL/6J mice and apolipoprotein E (ApoE)-deficient mice. In contrast, adherence of C. pneumoniae-infected macrophages to the aortas of intercellular adhesion molecule 1 (ICAM-1) knockout mice was not enhanced, suggesting that ICAM-1 is crucial for activation of the adherence of C. pneumoniae-infected macrophages to the endothelium. In conclusion, the present study defined a homing mechanism by which C. pneumoniae promotes the adherence of mononuclear phagocytes to the endothelium at the site of atherosclerotic lesion formation to promote the progression of atherosclerosis.
authors: Department of Pathobiology, University of Washington, Seattle, WA 98195, USA.
authors: Naohisa
authors: N
authors: Takaoka
authors: Lee Ann
authors: LA
authors: Campbell
authors: Amy
authors: A
authors: Lee
authors: Michael E
authors: ME
authors: Rosenfeld
authors: Cho-Chou
authors: CC
authors: Kuo
mesh_terms: D000818:Animals; D001011:Aorta / Q000382:microbiology*; D001057:Apolipoproteins E / Q000172:deficiency; D002448:Cell Adhesion; D002460:Cell Line; D002478:Cells, Cultured; D016993:Chlamydophila pneumoniae / Q000502:physiology*; D042783:Endothelial Cells / Q000382:microbiology*; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D008264:Macrophages / Q000382:microbiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic
chemical_list: D001057:Apolipoproteins E; D018799:Intercellular Adhesion Molecule-1