[Variances of leptin mRNA in the adipose tissue of NAFLD rats intervened with the extracts of Polygonum cuspidatum compound].
The NAFLD rats were intervened with the extracts of Polygonum cuspidatum compound for 4 weeks. The reverse transcription and real-time quantitative polymerase chain reaction (RT-qPCR) methods were used to detecte the relative level of leptin mRNA in the adipose tissue of intervenient and control groups. Their variances of fat and glucose in serum were detected with the biochemical methods. The results showed that the level of leptin mRNA of intervenient group was significantly increased (P <0.05) and the triglycered and total cholesterol were significantly decreased (P <0.05). The extracts of Polygonum cuspidatum compound could increase leptin mRNA level in adipose tissue and improve the fat metablolism in serum. However, the serum glucose of the intervenient group was a little raised (P>0.05).
Jiang
Qing-lan
QL
Core Lab, The First Municipal People's Hospital of Guangzhou, Guangzhou 510180, China.
Pan
Jin-yao
JY
Ma
Jun
J
Li
Yu-yuan
YY
Xu
Bang-lao
BL
D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005234:Fatty Liver / Q000097:blood / Q000235:genetics* / Q000473:pathology; D005260:Female; D000960:Hypolipidemic Agents / Q000494:pharmacology; D020738:Leptin / Q000096:biosynthesis / Q000235:genetics*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D000960:Hypolipidemic Agents; D020738:Leptin; D012333:RNA, Messenger; D014280:Triglycerides; D002784:Cholesterol