title: The fungus Ustilago maydis and humans share disease-related proteins that are not found in Saccharomyces cerevisiae.
abstract: BACKGROUND
  The corn smut fungus Ustilago maydis is a well-established model system for molecular phytopathology. In addition, it recently became evident that U. maydis and humans share proteins and cellular processes that are not found in the standard fungal model Saccharomyces cerevisiae. This prompted us to do a comparative analysis of the predicted proteome of U. maydis, S. cerevisiae and humans.
  
  
  RESULTS
  At a cut off at 20% identity over protein length, all three organisms share 1738 proteins, whereas both fungi share only 541 conserved proteins. Despite the evolutionary distance between U. maydis and humans, 777 proteins were shared. When applying a more stringent criterion (> or = 20% identity with a homologue in one organism over at least 50 amino acids and > or = 10% less in the other organism), we found 681 proteins for the comparison of U. maydis and humans, whereas the both fungi share only 622 fungal specific proteins. Finally, we found that S. cerevisiae and humans shared 312 proteins. In the U. maydis to H. sapiens homology set 454 proteins are functionally classified and 42 proteins are related to serious human diseases. However, a large portion of 222 proteins are of unknown function.
  
  
  CONCLUSIONS
  The fungus U. maydis has a long history of being a model system for understanding DNA recombination and repair, as well as molecular plant pathology. The identification of functionally un-characterized genes that are conserved in humans and U. maydis opens the door for experimental work, which promises new insight in the cell biology of the mammalian cell.
authors: Munich Information Center for Protein Sequences/Institute of Bioinformatics and Systems Biology, Helmholz Zentrum München, Germany. M.Muensterkoetter@helmholtz-muenchen.de
authors: Martin
authors: M
authors: Münsterkötter
authors: Gero
authors: G
authors: Steinberg
mesh_terms: D017124:Conserved Sequence; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D016681:Genome, Fungal; D015894:Genome, Human; D006801:Humans; D008957:Models, Genetic; D010935:Plant Diseases / Q000382:microbiology; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000235:genetics; D020543:Proteome; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014588:Ustilago / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D005656:Fungal Proteins; D011506:Proteins; D020543:Proteome