Survival and fertility rate of cooled dromedary camel spermatozoa supplemented with catalase enzyme.
The present study was planned to study the effects of addition of different concentrations of catalase enzyme (0, 250, 500 and 1,000 IU/ml) to cooled dromedary camel semen extended with tris-yolk-fructose extender on semen quality during storage at 5 C for up to 5 days. Conception rates of she-camels artificially inseminated with whole fresh or extended cooled dromedary camel semen with or without 500 IU/ml catalase enzyme were also estimated. The results showed that addition of catalase enzyme at concentrations of 250 or 500 IU/ml to extended cooled dromedary camel semen significantly increased (P<0.01) the percentage of sperm motility and significantly decreased (P<0.01) the percentages of dead spermatozoa, sperm abnormalities and acrosomal damage. The highest (P<0.01) percentage of sperm motility was recorded with extended cooled dromedary camel semen supplemented with catalase enzyme at a concentration of 500 IU/ml, and the lowest (P<0.01) value was recorded with catalase enzyme at a concentration of 1000 IU/ml. On the other hand, the lowest (P<0.01) percentages of dead spermatozoa, sperm abnormalities and acrosomal damage of spermatozoa were recorded with extended cooled dromedary camel semen supplemented with 500 IU/ml, and the highest (P<0.01) values were recorded with catalase enzyme at a concentration of 1,000 IU/ml. Advancement of the storage time at 5 C significantly decreased (P<0.01) the percentage of sperm motility and significantly increased (P<0.01) the percentages of dead spermatozoa, sperm abnormalities and acrosomal damage of spermatozoa. Moreover, the conception rates of she-camels artificially inseminated with whole fresh, extended cooled dromedary camel semen free-catalase enzyme and extended cooled dromedary camel semen supplemented with catalase enzyme at a concentration of 500 IU/ml were 46.15, 22.22 and 37.50%, respectively. In conclusion, the results show that addition of catalase enzyme at a concentration of 500 IU/ml to semen extender can be used as an agent for prolongation of dromedary camel sperm cell survival during storage at 5 C.
Medan
Mohamed Sabry
MS
Department of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt. medan69@hotmail.com
Absy
Gamal
G
Zeidan
Alaa Elsayed
AE
Khalil
Medhat Hussein
MH
Khalifa
Hesham Hussein
HH
Abdel-Salaam
Atef Mahrous
AM
Abdel-Khalek
Tarek Mohammad
TM
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002162:Camelus; D002374:Catalase / Q000494:pharmacology*; D003080:Cold Temperature; D005260:Female; D005306:Fertilization / Q000187:drug effects*; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D012661:Semen / Q000166:cytology; D012662:Semen Preservation / Q000662:veterinary*; D013094:Spermatozoa / Q000187:drug effects*
D000975:Antioxidants; D002374:Catalase