A gain-of-function mutation of transcriptional factor PTL results in curly leaves, dwarfism and male sterility by affecting auxin homeostasis.
GT factors are plant-specific trihelix DNA-binding transcription factors, which are involved in light responses and other developmental processes in plant. We identified a gain-of-function mutant of a GT-2 factor gene, PETAL LOSS (PTL), which displayed pleiotropic phenotypes including dwarfism, curly leaves, retarded growth and male sterility. We found that constitutive and ectopic over-expression of PTL driven by the CaMV 35S promoter could not recapitulate the phenotypes of the 35S enhancer-driven mutant ptl-D, and was lethal in some of the transgenic plants at the cotyledon developmental stage, suggesting that accurate temporal and spatial expression of PTL is essential for its proper functional implementation during plant development. Further analysis showed that ptl-D was defective in auxin action and that the alteration of auxin distribution corresponded to the curly leaf phenotype. The fact that degeneration of septum cells and subsequent breakage along the stomium was not observed in ptl-D anthers suggests that defective anther dehiscence was the cause for male sterility. Identification and characterization of the gain-of-function mutant ptl-D will improve our understanding of the diverse functions of GT factors during plant development.
Li
Xin
X
National Laboratory for Protein Engineering and Plant Genetic Engineering, Peking-Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
Qin
Genji
G
Chen
Zhangliang
Z
Gu
Hongya
H
Qu
Li-Jia
LJ
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000502:physiology; D005298:Fertility / Q000235:genetics; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006706:Homeostasis; D007210:Indoleacetic Acids / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D009154:Mutation; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology
D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; C528401:PTL protein, Arabidopsis; D018749:RNA, Plant; D014157:Transcription Factors; D005966:Glucuronidase