Pediocin SA-1, an antimicrobial peptide from Pediococcus acidilactici NRRL B5627: production conditions, purification and characterization.
Fermentation broths of Pediococcus acidilactici NRRL B5627 exhibited a certain antimicrobial activity due to a bacteriocin produced during early growth and until the stationary phase of growth was reached (at optimum of 60% dissolved oxygen saturation). Its size was determined by electrospray ionization mass spectrometric analysis as 3.660 kDa. N-terminal sequencing showed that the bacteriocin had 19 amino acid residues in the order KYYGXNGVXTXGKHSXVDX. The purified bacteriocin is similar to pediocins isolated by various Pediococci and therefore, it belongs to the class IIa of bacteriocins and is thus designated pediocin SA-1. Sensitivity of the purified pediocin to various storage temperatures and enzyme treatments was examined. Purified pediocin SA-1 is heat stable for up to 60 min at 121 degrees C. Pediocin SA-1 is inhibitory to several food-borne pathogens and food spoilage bacteria. It appears to be significantly more effective against Listeria spp. compared to pediocin PD-1 produced by P. damnosus. The mode of action of the purified bacteriocin appears to be bactericidal.
Anastasiadou
Sofia
S
Department of Hygiene and Technology of Food of Animal Origin, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki 54006, Greece.
Papagianni
Maria
M
Filiousis
George
G
Ambrosiadis
Ioannis
I
Koidis
Pavlos
P
D000595:Amino Acid Sequence; D001419:Bacteria / Q000145:classification / Q000187:drug effects; D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019149:Bioreactors; D004355:Drug Stability; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010376:Pediococcus / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D013816:Thermodynamics
D001430:Bacteriocins; D007773:Lactates; D010447:Peptide Hydrolases; D005947:Glucose