[Intervention of selenium on injured type 1 deiodinase by excessive iodine in mice].
OBJECTIVE
To study the mechanism of the damage resulted from iodine excess and to seek suitable selenium intervention dosage.


METHODS
160 BALB/c mice were divided into eight groups, the normal control group, the excessive iodine group (drunk the water containing potassium iodate 3000 microg/L) and six selenium groups (drunk the water containing potassium iodate 3000 microg/L and selenium 0.1, 0.2, 0.3, 0.4, 0.5 and 0.75 mg/L). The type 1 deiodinase (D1) activities and the levels of mRNA in liver, kidney and thyroid were determined by RT-PCR.


RESULTS
The mRNA levels of D1 restored to normal levels in all of the IS groups, while only 0.1-0.4 mg/L selenium supplement groups had normal activities of D1 in liver, kidney and thyroid.


CONCLUSIONS
Decline of D1 activities in liver, kidney and thyroid seems to be one of the reasons of the damage and should be chosen for effective intervention.
Xu
Jian
J
Shenzhen Chronic Disease Hospital, Shenzhen 518020, China. anniexu73@126.com
Yang
Xuefeng
X
Guo
Huailan
H
Liu
Xiaoli
X
D000818:Animals; D005260:Female; D007453:Iodide Peroxidase / Q000235:genetics / Q000378:metabolism*; D007455:Iodine / Q000008:administration & dosage* / Q000009:adverse effects* / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D012643:Selenium / Q000008:administration & dosage / Q000494:pharmacology*; D013961:Thyroid Gland / Q000201:enzymology
D012333:RNA, Messenger; D007455:Iodine; C109812:iodothyronine deiodinase type I; D007453:Iodide Peroxidase; D012643:Selenium