Cyclophilin A produced by thymocytes regulates the migration of murine bone marrow cells.
Supernatant obtained from high dose hydrocortisone resistant thymocytes can induce migration of the bone marrow cell precursors to the periphery. This biological activity depends on the presence of the 18 kDa protein, whose amino acid sequence fits with the sequence of the secretory form of murine cyclophilin A (SP-18). Cyclophilin A isolated from the supernatant of the cortisone-resistant thymoma EL-4 shows its characteristic functional features as it demonstrates isomerase activity and binds with cyclosporine A. The cyclophilin A obtained manifests chemotactic activity that regulates migration of bone marrow cell precursors of neutrophils, T-, B- and dendritic cells.
Khromykh
Ludmila M
LM
Laboratory of Regulatory Mechanisms in Immunity, Carcinogenesis Institute State Institution Russian N.N. Blokhin Cancer Research Center Russian Academy of Medical Sciences, Kashirskoe shosse 24, 115478 Moscow, Russia. lkhromykh@list.ru
Kulikova
Natalia L
NL
Anfalova
Tatiana V
TV
Muranova
Tatiana A
TA
Abramov
Vyacheslav M
VM
Vasiliev
Anatoliy M
AM
Khlebnikov
Valentin S
VS
Kazansky
Dmitriy B
DB
D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D039481:CD11b Antigen; D039521:CD11c Antigen; D045744:Cell Line, Tumor; D002465:Cell Movement; D002478:Cells, Cultured; D002633:Chemotaxis; D003348:Cortisone / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D021984:Cyclophilin A / Q000096:biosynthesis; D003713:Dendritic Cells / Q000502:physiology; D004351:Drug Resistance; D005260:Female; D006098:Granulocytes / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D054448:Precursor Cells, B-Lymphoid; D054504:Precursor Cells, T-Lymphoid / Q000502:physiology; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects
D039481:CD11b Antigen; D039521:CD11c Antigen; D017077:Culture Media, Conditioned; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D021984:Cyclophilin A; D003348:Cortisone