[The building of chip-based capillary electrophoresis platform and application].
OBJECTIVE
To detect the point mutations by chip-based capillary electrophoresis and to provide a rapid and sensitive technique detection for beta-thalassemia.


METHODS
Multiplex primer-extension reaction was used to amplify the common loci of the samples for beta-thalassemia. The reaction products were detected by the chip-based capillary electrophoresis and the genotypes of the samples were discrened.


RESULTS
A system was constructed to detect the point mutations of beta-thalassemia by chip-based capillary electrophoresis, and the technology was ralidated by the patients' samples and the results coincided with those of detection kit.


CONCLUSIONS
Beta-thalassemia can be detected by chip-based capillary electrophoresis rapidly with a small amount of samples. It would be a new detection method of the genetic disorders.
Hu
Hua
H
Department of Obstetrics & Gynecology, Xinan Hospital, the Third Military Medical University, Chongqing, 400038 PR China.
Xiong
Qiang
Q
Li
Cai-xia
CX
Gao
Hua-fang
HF
Yang
Qian
Q
Liang
Zhi-qing
ZQ
D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D030342:Genetic Diseases, Inborn / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D017086:beta-Thalassemia / Q000175:diagnosis* / Q000235:genetics