Reciprocal regulation of the human sterol regulatory element binding protein (SREBP)-1a promoter by Sp1 and EGR-1 transcription factors.
Sterol regulatory element binding protein (SREBP)-1a is a transcription factor that is highly expressed in actively growing cells, and is involved in the biosynthesis of cholesterol, fatty acids and phospholipids. We have mapped the minimal human SREBP-1a promoter region to 75bp upstream of the translation start site where we discovered a functional role for the 3 GC-boxes containing overlapping sites for the Sp1 and EGR-1 transcription factors. Intact SP1-binding sites are essential for promoter activity, whereas EGR-1 suppresses the transcription of the human SREBP-1a promoter. These results reveal a novel physiologically relevant transcriptional mechanism for the reciprocal regulation of the SREBP-1a expression.
Fernández-Alvarez
Ana
A
Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas, Jaime Roig 11, Valencia, Spain.
Tur
Gemma
G
López-Rodas
Gerardo
G
Casado
Marta
M
D001483:Base Sequence; D002460:Cell Line; D047369:Chromatin Immunoprecipitation; D051765:Early Growth Response Transcription Factors / Q000502:physiology*; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D016329:Sp1 Transcription Factor / Q000502:physiology*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics*
D051765:Early Growth Response Transcription Factors; D016329:Sp1 Transcription Factor; D051780:Sterol Regulatory Element Binding Protein 1