title: Probing microscopic chemical environments with high-intensity chirped pulses.
abstract: By varying the chirp of high-intensity pulses, we can use the chirp-condition-dependent fluorescence yield to distinguish among different molecules or the same molecule in different microenvironments. As an example of the latter we show that SNAFL-2, a well-known pH-sensitive dye, shows large modulation in fluorescence yield in response to both variation in acidity and variation in chirp condition. Future application of this technique as a novel contrast mechanism within fluorescence microscopy is discussed.
authors: A H
authors: AH
authors: Buist
authors: M
authors: M
authors: Mller
authors: R I
authors: RI
authors: Ghauharali
authors: G J
authors: GJ
authors: Brakenhoff
authors: J A
authors: JA
authors: Squier
authors: C J
authors: CJ
authors: Bardeen
authors: V V
authors: VV
authors: Yakovlev
authors: K R
authors: KR
authors: Wilson