title: Improved co-oxidation of beta-carotene to beta-ionone using xanthine oxidase-generated reactive oxygen species in a multiphasic system.
abstract: beta-Ionone, an aroma compound exhibiting flower notes, can be obtained from beta-carotene in a cooxidation system utilizing xanthine oxidase-generated reactive oxygen species (ROS). ROS have to be controlled as, although they can give rise to beta-ionone, they may also degrade it. In this work, the biotransformation of beta-carotene into beta-ionone was investigated in systems containing variable proportions of decane to extract beta-ionone before degradation. The use of 50% or 90% decane resulted in increased production yields. Tween 80, which was added to further improve the production, slightly decreased the reactivity of the medium and the extraction of beta-carotene, but increased the extraction of beta-ionone. In total, the addition of Tween 80 significantly improved the yields of conversion, which reached 34% with 50% decane and 2.5 g/L Tween 80 compared to 10% without decane and Tween 80. These results show that it is possible to control a ROS-mediated reaction by the addition of a solvent phase and by modifing the medium composition.
authors: Laboratoire de Génie des Procédés Microbiologiques et Alimentaires (GPMA), ENSBANA, Université de Bourgogne, Dijon, France.
authors: Mai Huong
authors: MH
authors: Ly
authors: Lan Cao
authors: LC
authors: Hoang
authors: Jean-Marc
authors: JM
authors: Belin
authors: Yves
authors: Y
authors: Waché
mesh_terms: D001709:Biotechnology / Q000379:methods; D008954:Models, Biological; D015394:Molecular Structure; D045792:Norisoprenoids / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D011136:Polysorbates / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism*; D019207:beta Carotene / Q000737:chemistry / Q000378:metabolism*
chemical_list: D045792:Norisoprenoids; D011136:Polysorbates; D017382:Reactive Oxygen Species; D019207:beta Carotene; C008157:beta-ionone; D014969:Xanthine Oxidase