Synthesis of Leishmania cap-4 intermediates, cap-2 and cap-3.
Synthesis of Leishmania mRNA 5'-cap analogs, m(7)Gpppm(2)(6)AmpAm (cap-2), and m(7)Gpppm(2)(6)AmpAmpCm (cap-3) is reported. Binding affinities of those cap analogs for LeishIF4E proteins were determined using fluorescence spectroscopy. Cap-3 showed similar affinity to LeishIF4Es compared to the mature trypanosomatids cap structure (cap-4).
Lewdorowicz
Magdalena
M
Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warsaw, Poland.
Stepinski
Janusz
J
Kierzek
Ryszard
R
Jemielity
Jacek
J
Zuberek
Joanna
J
Yoffe
Yael
Y
Shapira
Michal
M
Stolarski
Ryszard
R
Darzynkiewicz
Edward
E
D000818:Animals; D039561:Eukaryotic Initiation Factor-4E / Q000737:chemistry; D006158:Guanosine Pentaphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry; D007891:Leishmania / Q000378:metabolism*; D012314:RNA Cap Analogs / Q000138:chemical synthesis* / Q000737:chemistry; D012315:RNA Caps / Q000138:chemical synthesis* / Q000737:chemistry; D016053:RNA, Protozoan / Q000138:chemical synthesis* / Q000737:chemistry
D039561:Eukaryotic Initiation Factor-4E; D012314:RNA Cap Analogs; D012315:RNA Caps; D016053:RNA, Protozoan; D006158:Guanosine Pentaphosphate