title: Arabidopsis P-glycoprotein19 participates in the inhibition of gravitropism by gravacin.
abstract: ATP-binding cassette (ABC) transporters have been implicated in a multitude of biological pathways. In plants, some ABC transporters are involved in the polar transport of the plant hormone auxin and the gravitropic response. We previously identified Gravacin as a potent inhibitor of gravitropism in Arabidopsis thaliana. We demonstrate that P-glycoprotein19 (PGP19) is a target for Gravacin and participates in its inhibition of gravitropism. Gravacin inhibited the auxin transport activity of PGP19 and PGP19-PIN complexes. Furthermore, we identified E1174 as an important residue for PGP19 activity and its ability to form active transport complexes with PIN1. Gravacin is an auxin transport inhibitor that inhibits PGPs, particularly PGP19, which can be used to further dissect the role of PGP19 without the inhibition of other auxin transporters, namely PIN proteins.
authors: Department of Botany and Plant Sciences, University of California, Riverside, Riverside, CA 92521, USA.
authors: Marcela
authors: M
authors: Rojas-Pierce
authors: Boosaree
authors: B
authors: Titapiwatanakun
authors: Eun Ju
authors: EJ
authors: Sohn
authors: Fang
authors: F
authors: Fang
authors: Cynthia K
authors: CK
authors: Larive
authors: Joshua
authors: J
authors: Blakeslee
authors: Yan
authors: Y
authors: Cheng
authors: Sean R
authors: SR
authors: Cutler
authors: Sean
authors: S
authors: Cuttler
authors: Wendy A
authors: WA
authors: Peer
authors: Angus S
authors: AS
authors: Murphy
authors: Natasha V
authors: NV
authors: Raikhel
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D020346:Aquaporins / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D001667:Binding, Competitive / Q000502:physiology; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D005663:Furans / Q000378:metabolism / Q000494:pharmacology*; D018522:Gravitropism / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D018546:Hypocotyl / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D015394:Molecular Structure; D009154:Mutation; D010797:Phthalimides / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
chemical_list: D018528:ATP-Binding Cassette Transporters; D020346:Aquaporins; D029681:Arabidopsis Proteins; D005663:Furans; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; D010797:Phthalimides; D011994:Recombinant Proteins; C114215:ATPGP1 protein, Arabidopsis; C527265:Gravacin; C519873:PGP19 protein, Arabidopsis; C116363:PIN1 protein, Arabidopsis; C117013:AT5G57090 protein, Arabidopsis; C099402:delta-TIP protein, Arabidopsis; C002687:alpha-naphthylphthalamic acid