Optical tweezers as a probe for oligodeoxyribonucleotide structuration.
The aim of this work is to investigate if the optical tweezers (OT) are suitable as a diagnostic tool for monitoring the oligodeoxyribonucleotide (ODN) structural behavior in solution. Preliminary experiments, performed on the quadruplex formed by the ODN sequence TGGGGT, showed that the OT can be used as a probe for ODN structuration by monitoring the medium viscosity changes associated with ODN folding-unfolding processes.
Borbone
Nicola
N
Dipartimento di Chimica delle Sostanze Naturali, Università di Napoli Federico II, Faculty of Pharmacy, via Domenico Montesano 49, Naples 80131, Italy. borbone@unina.it
Oliviero
Giorgia
G
Amato
Jussara
J
Piccialli
Gennaro
G
Mayol
Luciano
L
Miccio
Lisa
L
Pescen
Giuseppe
G
Sasso
Antonio
A
D001483:Base Sequence; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D052898:Optical Tweezers; D012996:Solutions; D014783:Viscosity
D009838:Oligodeoxyribonucleotides; D012996:Solutions