title: Partial sequencing and expression of genes involved in glucose metabolism in adipose tissues and skeletal muscle of healthy cats.
abstract: Impaired insulin sensitivity is increasingly recognised in cats, but sequences of genes involved in insulin-signalling are largely undetermined in this species. In this study, extended feline mRNA sequences were determined for the adiponectin, glucose transporter-1 (GLUT1), GLUT4, peroxisome proliferative activated receptor-gamma1 (PPARgamma1), PPARgamma2, plasminogen activator inhibitor-1 (PAI-1), monocyte chemoattractant protein-1 (MCP-1) and insulin receptor genes. Conserved dog-specific primers identified from human-dog mRNA alignments were used to amplify feline cDNA in the polymerase chain reaction (PCR). The feline sequences determined by this method were used to design feline-specific primers suitable for real-time PCR for quantification of gene expression in insulin sensitive tissues of healthy cats. Partial sequences of feline mRNAs had 86-95% identity with dog and human genes. Expression of adiponectin, GLUT1, GLUT4, PPARgamma1, PPARgamma2, PAI-1 and insulin receptor mRNA was detected and quantified in subcutaneous and visceral fat and skeletal muscle, whereas MCP-1 mRNA was detected in adipose tissue but not in skeletal muscle. Further characterisation of genes related to glucose metabolism in cats will provide additional insights into insulin-signalling mechanisms in this species.
authors: Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zürich, Winterthurerstr. 260, 8057 Zürich, Switzerland. ezini@vetclinics.uzh.ch
authors: Eric
authors: E
authors: Zini
authors: Philippe
authors: P
authors: Linscheid
authors: Marco
authors: M
authors: Franchini
authors: Karin
authors: K
authors: Kaufmann
authors: Edouard
authors: E
authors: Monnais
authors: Annette P
authors: AP
authors: Kutter
authors: Mathias
authors: M
authors: Ackermann
authors: Thomas A
authors: TA
authors: Lutz
authors: Claudia E
authors: CE
authors: Reusch
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D002415:Cats / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism / Q000235:genetics*; D005260:Female; D015870:Gene Expression / Q000235:genetics; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000235:genetics*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D047495:PPAR gamma / Q000235:genetics; D011972:Receptor, Insulin / Q000235:genetics; D013045:Species Specificity
chemical_list: D001786:Blood Glucose; D051246:Glucose Transport Proteins, Facilitative; D007328:Insulin; D047495:PPAR gamma; D011972:Receptor, Insulin