Synthesis of polyester by means of genetic code reprogramming.
Here we report the ribosomal polymerization of alpha-hydroxy acids by means of genetic code reprogramming. The flexizyme system, a ribozyme-based tRNA acylation tool, was used to re-assign individual codons to seven types of alpha-hydroxy acids, and then polyesters were synthesized under controls of the reprogrammed genetic code using a reconstituted cell-free translation system. The sequence and length of the polyester segments were specified by the mRNA template, indicating that high-fidelity ribosome expression of polyesters was possible. This work opens a door for the mRNA-directed synthesis of backbone-altered biopolymers.
Ohta
Atsushi
A
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 113-8656 Tokyo, Japan.
Murakami
Hiroshi
H
Higashimura
Eri
E
Suga
Hiroaki
H
D001483:Base Sequence; D001704:Biopolymers / Q000096:biosynthesis* / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005815:Genetic Code / Q000235:genetics*; D006880:Hydroxy Acids / Q000737:chemistry / Q000378:metabolism; D009842:Oligopeptides; D010455:Peptides / Q000235:genetics; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001704:Biopolymers; D006880:Hydroxy Acids; D009842:Oligopeptides; D010455:Peptides; D011091:Polyesters; D012333:RNA, Messenger; D012343:RNA, Transfer; C085706:FLAG peptide