Genetic control of susceptibility to pulmonary infection with Chlamydia pneumoniae in the mouse.
A mouse model was used to study the genetic control of differential host response to pulmonary infection with Chlamydia pneumoniae. The A/J and C57BL/6 strains show differential response to intranasal infection with respect to their ability to clear pulmonary bacterial load and the extent of lung pathology developed by 2 weeks post infection. The genetic basis of this interstrain difference was studied by whole-genome scan in an informative [A/J x C57BL/6J] F2 cross using the pulmonary microbial load as a phenotypic readout of host response. We detected a highly significant linkage (LOD score=11.5) on chromosome 17 that overlaps with the major histocompatibility (MHC) locus. This quantitative trait locus (QTL) accounts for approximately 30% of the phenotypic variance with B6 alleles conferring susceptibility and inherited in a recessive fashion. Significant linkage was also detected to chromosome 5 in female mice, while chromosome 6 showed suggestive linkage in male mice, pointing to additional complexity in the genetic control of the difference in susceptibility observed in A/J and C57BL/6J.
Min-Oo
G
G
Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
Lindqvist
L
L
Vaglenov
A
A
Wang
C
C
Fortin
P
P
Li
Y
Y
Kaltenboeck
B
B
Gros
P
P
D000818:Animals; D016993:Chlamydophila pneumoniae / Q000235:genetics* / Q000472:pathogenicity; D033481:Chromosomes, Mammalian; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D008126:Lod Score; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D018895:Microsatellite Repeats; D009929:Organ Size; D040641:Quantitative Trait Loci; D012044:Regression Analysis; D012141:Respiratory Tract Infections / Q000235:genetics* / Q000382:microbiology; D012737:Sex Factors; D013045:Species Specificity
D005819:Genetic Markers