Optimization of HIV-1 infectivity assays.
HIV-1 reporter cell lines are the backbone of diagnostic assays, vaccine and drug development efforts. Performing HIV-1 infection experiments in a T cell background is desirable for many reasons. However, a low susceptibility to infection with primary patient isolates in available reporter T cell lines has limited such efforts. We here demonstrate that optimization of HIV-1 receptor expression and the utilization of serum free medium compositions can increase susceptibility of reporter T cell lines to HIV-1 infection by up to two orders of magnitude.
Jones
Jennifer
J
University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Whitford
William
W
Wagner
Frederic
F
Kutsch
Olaf
O
D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D003470:Culture Media; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans
D003470:Culture Media