[Profiles of intracellular expression, distribution, and activation of phospholipase A2 in host macrophages infected with Mycobacterium avium complex].
Our recent studies have shown that type IV cytosolic phospholipase A2 (cPLA2) plays an important role in the expression of macrophage (MPh) antimicrobial activity against the Mycobacterium avium complex (MAC). To clarify the modes of cPLA2 participation in MPh anti-MAC antimicrobial function in detail, we studied intracellular profiles of phospholilase A2, focusing on cPLA2, in MAC-infected MPhs, with the following findings: In murine peritoneal MPhs, cPLA2 was constitutively expressed even in uninfected MPhs, and MAC infection did not increase intramacrophage cPLA2 expression. In an RAW264.7 mouse MPh cell line (RAW264.7 MPhs) infected with MAC, a portion of intracellular cPLA2 was concentrated in MAC organisms residing within MPh phagosomes. MAC infection upregulated intramacrophage cPLA2 expression and induced its phosphorylation. These findings suggest that MAC infection of RAW264.7 MPhs may induce the activation of intracellular cPLA2, translocating it to phagosomes engulfing infected MAC organisms.
Sano
Chiaki
C
Department of Microbiology and Immunology, Shimane University School of Medicine.
Shimizu
Toshiaki
T
Tatano
Yutaka
Y
Yasumoto
Ko
K
Tomioka
Haruaki
H
D000818:Animals; D002460:Cell Line; D004789:Enzyme Activation; D006801:Humans; D008264:Macrophages / Q000201:enzymology* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D015270:Mycobacterium avium-intracellulare Infection / Q000201:enzymology*; D054467:Phospholipases A2 / Q000378:metabolism*
D054467:Phospholipases A2