title: Expression of oestrogen-related receptor alpha in human epidermis.
abstract: Skin is a non-classical target for oestrogens. Despite evidence showing that oestrogen receptors (ER) are expressed in skin, there are still extensive gaps in our understanding of how oestrogens exert their action in non-reproductive tissues. Oestrogen-related receptor-alpha (ERRalpha), orphan member of the nuclear receptor superfamily, shows a strong sequence homology with ERalpha but it does not bind oestradiol. Here, for the first time, we demonstrate ERRalpha expression in the epidermis of adult human skin. ERRalpha mRNA was detected in the epidermis of eight female donors using reverse transcriptase polymerase chain reaction. The presence of the protein was confirmed using western blotting and immunohistochemistry on 5 and 11 adult human skins, respectively. This study shows that ERRalpha is expressed in human epidermis and could intervene in a potentially new oestrogen signalling pathway in the skin.
authors: Laboratoire des Substituts Cutanés, CNRS UPR-412, Hospices civils de Lyon, Lyon, France.
authors: Elodie
authors: E
authors: Bertil
authors: Marie-Alexandrine
authors: MA
authors: Bolzinger
authors: Valérie
authors: V
authors: André
authors: Patricia
authors: P
authors: Rousselle
authors: Odile
authors: O
authors: Damour
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004817:Epidermis / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000097983:ERRalpha Estrogen-Related Receptor
chemical_list: D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D000097983:ERRalpha Estrogen-Related Receptor