The evaluation of maté (Ilex paraguariensis) genetic toxicity in human lymphocytes by the cytokinesis-block in the micronucleus assay.
The present study evaluates the clastogenic and/or aneugenic potential of maté (Ilex paraguariensis) - previously tested for the presence of 48 organophosphorous pesticides - in the culture of human lymphocytes in the absence of exogenous metabolic activation. Peripheral blood was obtained once from three healthy female donors for lymphocyte cell cultures. The cultures were treated with maté infusion (filtered in sterilized sartorius filter with a 0.22 mm pore membrane), distilled water (negative control), and 6 microg/ml bleomycin (positive control). For each experimental person, 3000 binucleated cells (BN) from two independent cultures (1000 cells from replicate cultures) were scored for the presence of micronuclei (MN). No statistical differences between maté infusion concentrations were observed: 1400 microg/ml (0.001+/-0.002), 700 microg/ml (0.0006+/-0.0015), 350 microg/ml (0.002+/-0.002), 175 microg/ml (0.002+/-0.003) and negative control (0.001+/-0.001). The present findings show that there is no clastogenic or/and aneugenic basis underlying maté action in the CBMN assay.
Alves
Rafael José Vargas
RJ
Laboratório da Toxicidade Genética (TOXIGEN), Programa de Pós Graduação em Genética e Toxicologia Aplicada (PPGGTA), Universidade Luterana do Brazil, Canoas, RS, Brazil.
Jotz
Geraldo Pereira
GP
do Amaral
Viviane Souza
VS
Montes
Tiago Maeso Hermes
TM
Menezes
Honório Sampaio
HS
de Andrade
Heloísa Helena Rodrigues
HH
D000328:Adult; D002478:Cells, Cultured; D019457:Chromosome Breakage / Q000187:drug effects; D048749:Cytokinesis / Q000187:drug effects*; D005260:Female; D006801:Humans; D030019:Ilex paraguariensis / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects*; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens; D009943:Organophosphorus Compounds / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry / Q000633:toxicity
D009153:Mutagens; D009943:Organophosphorus Compounds