Proliferation and neurogenesis of neural stem cells enhanced by cerebral microvascular endothelial cells.
In adult mammalian brain, vascular cells reside throughout life, close to central nervous system germinal zones, and neural stem cells (NSCs) mainly localize in the dentate gyrus of the hippocampus, subventricular zone, and olfactory bulb. Microvessels appear to produce a special microenvironment that may influence the characteristics of NSCs. To explore this potential correlation, an in vitro model with cocultured cerebral microvascular endothelial cells (CMECs) and NSCs was established in our study by using a transwell coculture system. The expression of nestin and NF in the early stage of coculture, and NF in the late stage, was detected by immunostaining. The results demonstrated that CMECs can stimulate self-renewal of NSCs and inhibit their differentiation, implying the potential of CMECs in promoting the neural differentiation of NSCs.
Guo
Ying
Y
Department of Neurosurgery, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China. gzguoying@hotmail.com
Shi
Dejin
D
Li
Wensheng
W
Liang
Chaofeng
C
Wang
Hui
H
Ye
Zhuopeng
Z
Hu
Liping
L
Wang
Hua-Qiao
HQ
Li
Yan
Y
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D054022:Cerebrum / Q000166:cytology*; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D016900:Neurofilament Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000502:physiology*
D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D016900:Neurofilament Proteins