[Selection of the microorganism for dioscin hydrolyze].
OBJECTIVE
To select the microorganism which can hydrolyze dioscin to diosgenin.


METHODS
The microorganism were selected from the surface of rhizome, rhizosphere soil, the inside of the leaves and rhizome of Dioscorea zingiberensis C. H. Wright. Diosgenin was identified by thin-layer chromatography and HPLC.


RESULTS
The microorganism which could hydrolyze dioscin from the experiment were identified as Aspergillus sp and Alternaria sp. Characteristics of enzymes production and fermentation technology of Aspergillus No. 1 were also studied primarily.


CONCLUSIONS
The Aspergillus strain No. 1 can secret enzyme to hydrolyze dioscin into diosgenin effectively.
Zhao
Yu-ting
YT
College of Life Science, Nanjing Normal University, Nanjing 210046, China.
Xu
Zeng-lai
ZL
Dai
Chuan-chao
CC
Tang
Xing-li
XL
Xia
Bing
B
Wang
Qiong
Q
D000528:Alternaria / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D001230:Aspergillus / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D027742:Dioscorea / Q000382:microbiology*; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000378:metabolism; D006868:Hydrolysis; D010946:Plants, Medicinal / Q000382:microbiology*; D027343:Rhizome / Q000382:microbiology
C019357:dioscin; D004144:Diosgenin