A membrane-bound form of IL-4 enhances proliferation and antigen presentation of CD40-activated human B cells.
CD40-activated B (CD40-B) cells might be an attractive source of autologous antigen-presenting cells (APCs) for immunotherapy due to the ability to obtain them from peripheral blood and expand them in vitro. However, soluble IL-4 (sIL-4) in B-cell culture may not represent the "immunological synapse" between B and CD4+ T cells. In this study, the K562 cell line, which expresses CD40L and membrane-bound IL-4 (mbIL-4), could induce higher B-cell proliferation and antigen-presenting surface molecules, including adhesion, costimulatory and HLA molecules, compared with sIL-4. The differentiation to plasmablasts was decreased in CD40-B cells treated with mbIL-4 (CD40-B/mbIL-4) based on flow cytometry analysis. Furthermore, CD40-B/mbIL-4 cells were as potent as mature dendritic cells in the allogeneic lymphocyte reaction and the ability to generate cytotoxic T lymphocytes specific for cytomegalovirus pp65 antigen in vitro. Our results suggest that mbIL-4 could be used to generate CD40-B cells as potent APCs for cellular vaccines and adoptive immunotherapy.
Park
Jung-Yong
JY
Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea.
Yoon
Sung Hee
SH
Kim
Eun-Kyung
EK
Yun
Sun-Ok
SO
Park
Mi-Young
MY
Sohn
Hyun-Jung
HJ
Kim
Tai-Gyu
TG
D017951:Antigen Presentation; D000956:Antigens, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D019013:CD40 Antigens / Q000276:immunology; D023201:CD40 Ligand / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D049109:Cell Proliferation; D006801:Humans; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D020014:K562 Cells; D008213:Lymphocyte Activation; D010750:Phosphoproteins / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D014162:Transfection; D014763:Viral Matrix Proteins / Q000276:immunology
D000956:Antigens, Viral; D019013:CD40 Antigens; D010750:Phosphoproteins; D011994:Recombinant Proteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa; D023201:CD40 Ligand; D015847:Interleukin-4