title: Establishment of hybrid cell lines producing monoclonal antibodies to a synthetic peptide from the E1 region of the hepatitis C virus.
abstract: We aimed at establishing hybridoma cells secreting monoclonal antibodies (mAbs) against E1 synthetic peptide of HCV. BALB/c mice were immunized with HCV E1-synthetic peptide (GHRMAWDMM) and its spleenocytes were fused with the P3NS1 myeloma cell line. Two highly reactive and specific mAbs (10C7 IgG2b mAb, and 10B2 IgG1 mAb) were generated. The target HCV E1 antigen was identified at approximately 38 kDa in serum of infected individuals. A newly developed ELISA detected the target antigen in 90% of sera from HCV RNA infected individuals with a specificity of 84%. So, the generated mAbs may provide promising probes for serodiagnosis of HCV infection.
authors: Department of Biomedical Technology, National Research Center, Cairo, Egypt. ashraftabll@yahoo.com
authors: Ashraf A
authors: AA
authors: Tabll
authors: Samy B
authors: SB
authors: Khalil
authors: Reem M
authors: RM
authors: El-Shenawy
authors: Gamal
authors: G
authors: Esmat
authors: Amr
authors: A
authors: Helmy
authors: Abdel Fattah
authors: AF
authors: Attallah
authors: Mostafa K
authors: MK
authors: El-Awady
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006526:Hepatitis C / Q000175:diagnosis*; D018936:Hepatitis C Antigens / Q000097:blood / Q000737:chemistry / Q000276:immunology; D006801:Humans; D006825:Hybridomas; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D012698:Serologic Tests; D014759:Viral Envelope Proteins / Q000097:blood* / Q000737:chemistry / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; C087035:E1 protein, Hepatitis C virus; D018936:Hepatitis C Antigens; D010455:Peptides; D014759:Viral Envelope Proteins