title: Analysis of cellular responses to Mycoplasma mycoides subsp. mycoides small colony biotype associated with control of contagious bovine pleuropneumonia.
abstract: A better understanding of protective immune memory against contagious bovine pleuropneumonia (CBPP) is needed in order to facilitate the development of safer vaccines based on selected components of the pathogen. For this purpose, cells collected from lymph nodes draining the lungs of Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC)-infected cattle were stimulated with the pathogen in vitro and evaluated concurrently for proliferation (CFSE based method), expression of activation, memory markers and cytokine production. Direct evidence is presented for a major contribution of CD4+ T cells to the vigorous proliferative and T1 biased cytokine recall responses observed in cattle that have recovered from infection but not in animals developing the acute form of the disease. Two different phenotypes of MmmSC-specific memory CD4 were observed based on CD62L expression and proliferative capacities. Furthermore, recall proliferation of B cells also occurred but was strictly dependent on the presence of CD4. The information provided in this study will facilitate the search for MmmSC antigens that have potential for the development of subunit vaccines against CBPP.
authors: Cirad, UPR Contrôle des maladies, F-34398 Montpellier, France. philippe.totte@cirad.fr
authors: Philippe
authors: P
authors: Totté
authors: Valérie
authors: V
authors: Rodrigues
authors: Aboubakar
authors: A
authors: Yaya
authors: Bamanga
authors: B
authors: Hamadou
authors: Ousmane
authors: O
authors: Cisse
authors: Mahamadou
authors: M
authors: Diallo
authors: Mamadou
authors: M
authors: Niang
authors: François
authors: F
authors: Thiaucourt
authors: Laurence
authors: L
authors: Dedieu
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D007111:Immunity, Cellular; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008297:Male; D009176:Mycoplasma mycoides / Q000276:immunology*; D011002:Pleuropneumonia, Contagious / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary*
chemical_list: D016207:Cytokines