GC-MS determined cotinine in an epidemiological study on smoking status at delivery.
The objective of this study was to measure the plasma cotinine levels in pregnant women and their newborns using a gas chromatography-mass spectrometry (GC-MS) method in an epidemiological-delivered population with a wide range of tobacco intakes. Nearly 1000 pregnant women from regional maternity wards (n=1007) were selected for the study. Each patient kept a tobacco diary and underwent a blood test to assess cotinine levels and at the same time that the newborns' cordonal plasma was taken. These values were then cross-checked. Cotinine was estimated using a selected-ion monitoring mode with a 1.5 ng/ml quantification limit. The cotinine levels in mothers and newborns were highly correlated, whatever the mother's smoking status, with a calculated cut-off for cotinine levels in active smokers of 21.5 ng/ml. Finally, the cotinine determined through this GC-MS method offered a sensitive and accurate measure of tobacco exposition of the pregnant women and their babies.
Chazeron
Ingrid de
Id
Department of Psychiatry B, Centre Hospitalier Universitaire, Rue Montalembert BP 69, F-63003 Clermont-Ferrand Cedex 1, France.
Daval
Sandrine
S
Ughetto
Sylvie
S
Richard
Damien
D
Nicolay
Alain
A
Lemery
Didier
D
Llorca
Pierre M
PM
Coudoré
François
F
D000328:Adult; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003367:Cotinine / Q000097:blood*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007231:Infant, Newborn; D011247:Pregnancy; D011248:Pregnancy Complications / Q000097:blood*; D012372:ROC Curve; D015203:Reproducibility of Results; D012907:Smoking / Q000097:blood / Q000453:epidemiology*
D003367:Cotinine