Mass spectrometry based methods for analysis of nucleosides as antiviral drugs and potential tumor biomarkers.
The intracellular analysis of the phosphorylated metabolites of some anti-HIV nucleosides by liquid chromatography or capillary electrophoresis coupled with tandem mass spectrometry (LC-MS/MS or CE-MS/MS) has been realized on human peripheral blood mononuclear cells (PBMC), with limit of quantitation (LOQ) that allow them to be quantitated intracellularly. We described also the analysis of modified urinary nucleosides as potential tumor biomarkers.
Agrofoglio
Luigi A
LA
Institut de Chimie Organique et Analytique, UMR CNRS 6005, Université d'Orléans, 45067 Orléans Cedex 2, France. luigi.agrofoglio@univ-orleans.fr
Bezy
Vincent
V
Chaimbault
Patrick
P
Delépée
Raphaël
R
Rhourri
Boutaina
B
Morin
Philippe
P
D019380:Anti-HIV Agents / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis / Q000652:urine*; D002853:Chromatography, Liquid; D019075:Electrophoresis, Capillary; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D009705:Nucleosides / Q000032:analysis* / Q000652:urine; D053719:Tandem Mass Spectrometry / Q000379:methods*
D019380:Anti-HIV Agents; D014408:Biomarkers, Tumor; D009705:Nucleosides