Loss of Hugl-1 expression associates with lymph node metastasis in endometrial cancer.
Mutation of neoplastic tumor suppressor genes, scribble, discs large, and lethal giant larvae (lgl), causes disruption of cell polarity and overproliferation of Drosophila epithelial cells and neuroblasts. Reduced expression of human homologue of lgl, Hugl-1, has been reported to be involved in development and progression of human colon cancer and malignant melanoma. To explore the association between Hugl-1 expression and clinical character in endometrial cancer, we examined the expression of Hugl-1 in primary endometrial cancer tissues. The expression of Hugl-1 mRNA in 86 primary endometrial cancer tissues was examined using semiquantitative reverse transcription polymerase chain reaction (RT-PCR). All samples were categorized into two groups: Hugl-1 positive and Hugl-1 negative. Clinical data of each group were analyzed by Fisher's exact probability test and survival rates of each group were compared by Kaplan-Meier method and Log-rank test. Loss of Hugl-1 expression had correlation with the higher incidence of lymph node metastasis, but not to the patient's age at onset, distant metastasis, clinical stage, lymph or venous vessel invasion, or histopathological grade of differentiation. The Hugl-1-positive group had poorer prognosis compared with the Hugl-1-negative group. These results indicate that loss of Hugl-1 expression in endometrial cancer may contribute to lymph node metastasis and it can be a factor of poor prognosis.
Tsuruga
Tetsushi
T
Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-8655, Japan.
Nakagawa
Shunsuke
S
Watanabe
Michiko
M
Takizawa
Shin
S
Matsumoto
Yoko
Y
Nagasaka
Kazunori
K
Sone
Kenbun
K
Hiraike
Haruko
H
Miyamoto
Yuichiro
Y
Hiraike
Osamu
O
Minaguchi
Takeo
T
Oda
Katsutoshi
K
Yasugi
Toshiharu
T
Yano
Tetsu
T
Taketani
Yuji
Y
D003598:Cytoskeletal Proteins / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D011379:Prognosis
D003598:Cytoskeletal Proteins; C097906:LLGL1 protein, human