HLA Class II-specific antibodies can react with T cells in flow cytometry crossmatch: a case report.
HLA Class I-specific antibodies are usually detected using a flow cytometry HLA crossmatch using T cells. A positive case is shown as a single, right-shifted peak on the anti-IgG FITC histogram of T cells. We report a case showing 2 peaks occurring concurrently, both positive and negative. The positive peak resulted from the binding of HLA Class II-specific antibodies to donor-activated T cells expressing the HLA-DR antigens. This case suggested donor-specific HLA Class II-specific antibodies can bind to some T cells as well as to whole B cells.
Heo
W B
WB
Department of Clinical Pathology, Kyungpook National University School of Medicine, Daegu, Republic of Korea.
Kim
C D
CD
Won
D I
DI
D005260:Female; D005434:Flow Cytometry; D005921:Glomerulonephritis / Q000276:immunology; D006681:HLA-D Antigens / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D017710:Lymphocyte Transfusion; D008875:Middle Aged; D010951:Plasma Exchange; D006435:Renal Dialysis; D013601:T-Lymphocytes / Q000276:immunology*; D016896:Treatment Outcome
D006681:HLA-D Antigens; D006684:HLA-DR Antigens