Relative influence of the adeno-associated virus (AAV) type 2 p5 element for recombinant AAV vector site-specific integration.
The p5 promoter region of the adeno-associated virus type 2 (AAV-2) rep gene has been described as essential for Rep-mediated site-specific integration (RMSSI) of plasmid sequences in human chromosome 19. We report here that insertion of a full-length or minimal p5 element between the viral inverted terminal repeats does not significantly increase RMSSI of a recombinant AAV (rAAV) vector after infection of growth-arrested or proliferating human cells. This result suggests that the p5 element may not improve RMSSI of rAAV vectors in vivo.
Guilbaud
Mickaël
M
INSERM U758, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, F-69007 Lyon, France.
Chadeuf
Gilliane
G
Avolio
Fabio
F
François
Achille
A
Moullier
Philippe
P
Recchia
Alessandra
A
Salvetti
Anna
A
D001483:Base Sequence; D002888:Chromosomes, Human, Pair 19; D017931:DNA Primers; D000229:Dependovirus / Q000235:genetics*; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D010957:Plasmids; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D016662:Virus Integration
D017931:DNA Primers