title: Characterization of xylanase from Lentinus edodes M290 cultured on waste mushroom logs.
abstract: Extracellular enzymes from Lentinus edodes M290 on normal woods (Quercus mongolica) and waste logs from oak mushroom production were comparatively investigated. Endoglucanase, cellobiohydrolase, beta-glucosidase, and xylanase activities were higher on waste mushroom logs than on normal woods after L. edodes M290 inoculation. Xylanase activity was especially different, with a three times higher activity on waste mushroom logs. When the waste mushroom logs were used as a carbon source, a new 35 kDa protein appeared. After the purification, the optimal pH and temperature for xylanase activity were determined to be 4.0 and 50 degrees C, respectively. More than 50% of the optimal xylanase activity was retained when the temperature was increased from 20 to 60 degrees C, after a 240 min reaction. At 40 degrees C, the xylanase maintained 93% of the optimal activity, after a 240 min reaction. The purified xylanase showed a very high homology to the xylanase family 10 from Aspergillus terreus by LC/MS-MS analysis. The highest Xcorr (1.737) was obtained from the peptide KWI SQGIPIDGIG SQTHLGSGGS WTVK originated from Aspergillus terreus, indicating that the 35 kDa protein was xylanase. This protein showed low homology to a previously reported L. edodes xylanase sequence.
authors: Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea.
authors: Jae-Won
authors: JW
authors: Lee
authors: Ki-Seob
authors: KS
authors: Gwak
authors: Su-Il
authors: SI
authors: Kim
authors: Mihyang
authors: M
authors: Kim
authors: Don-Ha
authors: DH
authors: Choi
authors: In-Gyu
authors: IG
authors: Choi
mesh_terms: D000363:Agaricales; D000595:Amino Acid Sequence; D018533:Biomass; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D020942:Shiitake Mushrooms / Q000201:enzymology* / Q000254:growth & development; D013696:Temperature
chemical_list: D043364:Endo-1,4-beta Xylanases