Novel monolithic poly(p-methylstyrene-co-bis(p-vinylbenzyl)dimethylsilane) capillary columns for biopolymer separation.
Hydrophobic organo-silane based monolithic capillary columns were prepared by thermally initiated free radical polymerisation within the confines of 200 microm i.d. fused silica capillaries. A novel crosslinker, namely bis(p-vinylbenzyl)dimethylsilane (BVBDMS), was copolymerised with p-methylstyrene (MS) in the presence of 2-propanol and toluene, using alpha,alpha'-azoisobutyronitrile (AIBN) as initiator. Monolithic capillary columns, differing in the total monomer, microporogen content and microporogen nature were fabricated and the chromatographic efficiency of each monolith, regarding the separation of proteins, peptides and oligonucleotides, was evaluated and compared. Changes in monolith morphology were monitored by scanning electron microscopy (SEM). Porosity and specific surface areas of the supports were studied by means of mercury intrusion porosimetry and BET measurements, respectively. Pressure drop vs. flow rate measurements proved the prepared poly(p-methylstyrene-co-bis(p-vinylbenzyl)dimethylsilane) (MS/BVBDMS) monoliths to be mechanically stable and swelling propensity (SP) factors of 0.78-1.10 indicate high crosslinking homogeneity.
Wieder
Wolfgang
W
Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innrain 52a, 6020 Innsbruck, Austria.
Lubbad
Said H
SH
Trojer
Lukas
L
Bisjak
Clemens P
CP
Bonn
Guenther K
GK
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D004355:Drug Stability; D008855:Microscopy, Electron, Scanning; D009841:Oligonucleotides / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification; D011137:Polystyrenes / Q000138:chemical synthesis* / Q000737:chemistry; D016062:Porosity; D011506:Proteins / Q000302:isolation & purification*; D012821:Silanes / Q000138:chemical synthesis* / Q000737:chemistry; D014753:Vinyl Compounds / Q000138:chemical synthesis* / Q000737:chemistry
D009841:Oligonucleotides; D010455:Peptides; D011137:Polystyrenes; D011506:Proteins; D012821:Silanes; D014753:Vinyl Compounds; C521540:poly((trimethylsilyl-4-methylstyrene)-co- bis(4-vinylbenzyl)dimethylsilane)