title: The role of disulfide bond isomerase A (DsbA) of Escherichia coli O157:H7 in biofilm formation and virulence.
abstract: The role of periplasmic disulfide oxidoreductase DsbA in Shiga toxin-producing Escherichia coli O157:H7 (STEC) was investigated. Deletion of dsbA (DeltadsbA) significantly decreased cell motility and alkaline phosphatase activity in STEC. STEC DeltadsbA also showed greater sensitivity to menadione and under low pH conditions. Significant reductions in surface attachment to both biotic (HT-29 epithelial cells) and abiotic (polystyrene and polyvinyl chloride) surfaces were observed in STEC DeltadsbA. In addition, no biofilm formation was detected in STEC DeltadsbA compared to wild-type cells in glass capillary tubes under continuous flow-culture system conditions. In the nematode model Caenorhabditis elegans-killing assay, the deletion of dsbA in STEC resulted in attenuated virulence compared to wild-type cells. STEC DeltadsbA was also found to have a reduced ability to colonize the nematode gut. These results suggest that DsbA plays important roles in biofilm formation and virulence in STEC cells.
authors: Yunho
authors: Y
authors: Lee
authors: Younghoon
authors: Y
authors: Kim
authors: Sujin
authors: S
authors: Yeom
authors: Saehun
authors: S
authors: Kim
authors: Sungsu
authors: S
authors: Park
authors: Che Ok
authors: CO
authors: Jeon
authors: Woojun
authors: W
authors: Park
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics / Q000502:physiology; D018441:Biofilms / Q000254:growth & development*; D015152:Blotting, Northern; D017173:Caenorhabditis elegans / Q000382:microbiology; D045744:Cell Line, Tumor; D019453:Escherichia coli O157 / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D009154:Mutation; D019704:Protein Disulfide-Isomerases / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D014774:Virulence / Q000235:genetics; D024483:Vitamin K 3 / Q000494:pharmacology; D000097573:Red Fluorescent Protein
chemical_list: D000469:Alkaline Phosphatase; D029968:Escherichia coli Proteins; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D019704:Protein Disulfide-Isomerases; D024483:Vitamin K 3; D000097573:Red Fluorescent Protein