Comparative role of antibiotic and proton pump inhibitor in experimental Clostridium difficile infection in mice.
Clostridium difficile inoculated BALB/c mice were investigated to assess the comparative role of antibiotic and proton pump inhibitor. They were examined for colonization and toxin production by C. difficile as well as myeloperoxidase activity and histopathological changes in the intestinal tract. The C. difficile count, toxin A and B titres and myeloperoxidase activity were significantly higher (P>0.05) in ampicillin and lansoprazole receiving groups as compared to the control and the C. difficile receiving groups. Similarly they showed significant difference (P >0.05) for epithelial damage, oedema and neutrophilic infiltrate in colons. In addition to antibiotic, PPI also acts as an independent risk factor for C. difficile infection in experimental studies.
Kaur
Sukhminderjit
S
Department of Gastroenterology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Vaishnavi
Chetana
C
Prasad
Kaushal Kishore
KK
Ray
Pallab
P
Kochhar
Rakesh
R
D053799:2-Pyridinylmethylsulfinylbenzimidazoles / Q000008:administration & dosage / Q000494:pharmacology; D000667:Ampicillin / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000096:biosynthesis; D016360:Clostridioides difficile / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D015169:Colony Count, Microbial; D004761:Enterocolitis, Pseudomembranous / Q000382:microbiology*; D004768:Enterotoxins / Q000096:biosynthesis; D007422:Intestines / Q000737:chemistry / Q000473:pathology; D064747:Lansoprazole; D051379:Mice; D008807:Mice, Inbred BALB C; D009195:Peroxidase / Q000032:analysis; D054328:Proton Pump Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000900:Anti-Bacterial Agents; D001427:Bacterial Toxins; C488583:Clostridium difficile lethal toxin B; D004768:Enterotoxins; D054328:Proton Pump Inhibitors; C041348:tcdA protein, Clostridium difficile; D064747:Lansoprazole; D000667:Ampicillin; D009195:Peroxidase