title: Inhibition of human ovarian tumor growth by cytokine-induced killer cells.
abstract: Despite the recent improvement in the treatment of ovarian cancer, this disease is still leading cause of cancer death in women. In this study, the anti-tumor activity of cytokine-induced killer (CIK) cells against human ovarian cancer was evaluated in vitro and in vivo. Although CD3+CD56+ cells were rare in fresh human peripheral blood mononuclear cells, they could expand more than 1,000-fold on day 14 in the presence of anti-CD3 antibody plus IL-2. At an effector-target cell ratio of 30:1, CIK cells destroyed 45% of SK-OV-3 human ovarian cancer cells, which was determined by the 51Cr-release assay. In addition, CIK cells at a dose of 23 million cells per mouse inhibited 73% of SK-OV-3 tumor growth in nude mouse xenograft assay. This study suggests that CIK cells may be used as an adoptive immunotherapy for patients with ovarian cancer.
authors: Bioevaluation Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang, Chungbuk 363-883, Korea.
authors: Hwan Mook
authors: HM
authors: Kim
authors: Jong Soon
authors: JS
authors: Kang
authors: Jaeseung
authors: J
authors: Lim
authors: Song-Kyu
authors: SK
authors: Park
authors: Kiho
authors: K
authors: Lee
authors: Yeo Dae
authors: YD
authors: Yoon
authors: Chang Woo
authors: CW
authors: Lee
authors: Ki Hoon
authors: KH
authors: Lee
authors: Gyoonhee
authors: G
authors: Han
authors: Kyu-Hwan
authors: KH
authors: Yang
authors: Yeon Jin
authors: YJ
authors: Kim
authors: Youngsoo
authors: Y
authors: Kim
authors: Sang-Bae
authors: SB
authors: Han
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D016207:Cytokines / Q000494:pharmacology*; D005260:Female; D006801:Humans; D016219:Immunotherapy, Adoptive; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D010051:Ovarian Neoplasms / Q000473:pathology / Q000628:therapy*
chemical_list: D016207:Cytokines