Galactosylation does not prevent the rapid clearance of long-term, 4 degrees C-stored platelets.
Cold storage of platelets for transfusion is desirable to extend platelet storage times and to prevent bacterial growth. However, the rapid clearance of cold-stored platelets prevents their use. A novel method for preventing the rapid clearance of cold-stored platelets has previously been developed in a murine model. Cold storage induces the clustering and recognition of exposed beta-N-acetylglucosamine (betaGlcNAc) on platelet surfaces. Glycosylation of betaGlcNAc residues with uridine 5'-diphosphogalactose (UDP-galactose) results in the normal survival of short-term (2 h) 0 degrees C-stored murine platelets. Based on this finding, we developed a similar glycosylation process by adding UDP-galactose to human apheresis platelets. A phase 1 clinical trial was conducted transfusing radiolabeled autologous apheresis platelets stored for 48 hours at 4 degrees C with or without pretreatment with UDP-galactose. In contrast to the murine study, galactosylation of human platelets did not prevent the accelerated platelet clearance routinely observed after 4 degrees C storage. We next developed a murine model of platelet storage for 48 hours at 4 degrees C and showed that UDP-galactose treatment of murine platelets also did not prevent their rapid clearance, in agreement with the human platelet study. We conclude that different mechanisms of clearance may exist for short- and long-term cold-stored platelets.
Wandall
Hans H
HH
ZymeQuest, Beverly, MA, USA.
Hoffmeister
Karin M
KM
Sørensen
Anne Louise
AL
Rumjantseva
Viktoria
V
Clausen
Henrik
H
Hartwig
John H
JH
Slichter
Sherrill J
SJ
D000818:Animals; D001781:Blood Component Removal; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D003080:Cold Temperature; D005260:Female; D005690:Galactose / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017713:Platelet Transfusion; D013997:Time Factors; D014531:Uridine Diphosphate Galactose / Q000494:pharmacology
D014531:Uridine Diphosphate Galactose; D005690:Galactose