title: Antioxidant and antimutagenic properties of the monoterpene indole alkaloid psychollatine and the crude foliar extract of Psychotria umbellata Vell.
abstract: Psychollatine is a monoterpene indole alkaloid produced and accumulated by Psychotria umbellata Vell. (Rubiaceae) leaves in relatively high amounts (approximately 3% of the dry weight). The alkaloid has been shown to display opioid-like analgesic, anxiolytic, antidepressive and antipsychotic activities in rodents. In vitro assays suggested a protective role for this molecule in plant oxidative stress responses. This work reports antioxidant properties of psychollatine and the crude foliar extract from P. umbellata in strains of Saccharomyces cerevisiae proficient and deficient in antioxidant defenses exposed to H2O2 and paraquat. The antimutagenic activity of P. umbellata and its main alkaloid were assayed in S. cerevisiae N123 strain in presence of H2O2. Moreover, the antioxidant capacity of these substances on the hydroxyl radical (OH.) was investigated, using the hypoxanthine/xanthine oxidase assay. Psychollatine and the crude foliar extract of P. umbellata showed protective effect against oxidative stress in yeast, acting both as antioxidant and antimutagenic agents.
authors: Departamento de Botânica/Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors: Variluska
authors: V
authors: Fragoso
authors: Naíla Cannes
authors: NC
authors: do Nascimento
authors: Dinara Jaqueline
authors: DJ
authors: Moura
authors: Ana Catarina Romano
authors: AC
authors: e Silva
authors: Marc François
authors: MF
authors: Richter
authors: Jenifer
authors: J
authors: Saffi
authors: Arthur Germano
authors: AG
authors: Fett-Neto
mesh_terms: D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002455:Cell Division / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media; D006027:Glycosides / Q000494:pharmacology*; D006540:Herbicides / Q000037:antagonists & inhibitors / Q000633:toxicity; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity; D026121:Indole Alkaloids / Q000494:pharmacology*; D016877:Oxidants / Q000633:toxicity; D010269:Paraquat / Q000037:antagonists & inhibitors / Q000633:toxicity; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D032069:Psychotria / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D003470:Culture Media; D006027:Glycosides; D006540:Herbicides; D026121:Indole Alkaloids; D016877:Oxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C499739:psychollatine; D006861:Hydrogen Peroxide; D014969:Xanthine Oxidase; D010269:Paraquat