title: Fluorescent high-performance liquid chromatographic analysis of vigabatrin enantiomers after derivatizing with naproxen acyl chloride.
abstract: Vigabatrin is widely used as an anticonvulsant in the treatment of seizures. Vigabatrin is usually supplied as racemate in formulation, but only the (S)-(+)-enantiomer of vigabatrin is pharmacologically active. A simple and sensitive liquid chromatographic method is described for the separation and quantification of vigabatrin enantiomers. The method is based on derivatizing racemic vigabatrin with a fluorescent chiral reagent (naproxen acyl chloride). The resulting diastereomeric derivatives are highly responsive to a fluorimetric detector (lambda(ex)=230 nm, lambda(em)=350 nm). The lower quantitation limit of the method is attainable at 25 nM for (S)-(+)-vigabatrin or (R)-(-)-vigabatrin with a detection limit of about 2.5 nM (S/N=3 with 10 microl injected). Application of the method to the analysis of vigabatrin in serum of dosed patients proved feasible.
authors: Graduate Institute of Pharmaceutical Sciences, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors: Chun-Yu
authors: CY
authors: Hsieh
authors: Shing-Yaw
authors: SY
authors: Wang
authors: Aij-Lie
authors: AL
authors: Kwan
authors: Hsin-Lung
authors: HL
authors: Wu
mesh_terms: D000927:Anticonvulsants / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009288:Naproxen / Q000031:analogs & derivatives / Q000737:chemistry; D013237:Stereoisomerism; D020888:Vigabatrin / Q000032:analysis* / Q000097:blood / Q000737:chemistry
chemical_list: D000927:Anticonvulsants; D009288:Naproxen; D020888:Vigabatrin