Amplified electrochemical detection of DNA through the aggregation of Au nanoparticles on electrodes and the incorporation of methylene blue into the DNA-crosslinked structure.
The amplified electrochemical sensing of DNA is accomplished by the analyte-induced aggregation of nucleic acid-functionalized Au nanoparticles, deposition of the aggregates on a thiolated monolayer-functionalized electrode, and the voltammetric analysis of the redox-active methylene blue intercalated in the nucleic acid duplexes associated with the aggregates.
Li
Di
D
Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Yan
Yiming
Y
Wieckowska
Agnieszka
A
Willner
Itamar
I
D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008751:Methylene Blue / Q000737:chemistry*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties
D003432:Cross-Linking Reagents; D013438:Sulfhydryl Compounds; D006046:Gold; D004247:DNA; D008751:Methylene Blue