title: A microsatellite genetic map of the turbot (Scophthalmus maximus).
abstract: A consensus microsatellite-based linkage map of the turbot (Scophthalmus maximus) was constructed from two unrelated families. The mapping panel was derived from a gynogenetic family of 96 haploid embryos and a biparental diploid family of 85 full-sib progeny with known linkage phase. A total of 242 microsatellites were mapped in 26 linkage groups, six markers remaining unlinked. The consensus map length was 1343.2 cM, with an average distance between markers of 6.5 +/- 0.5 cM. Similar length of female and male maps was evidenced. However, the mean recombination at common intervals throughout the genome revealed significant differences between sexes, approximately 1.6 times higher in the female than in the male. The comparison of turbot microsatellite flanking sequences against the Tetraodon nigroviridis genome revealed 55 significant matches, with a mean length of 102 bp and high sequence similarity (81-100%). The comparative mapping revealed significant syntenic regions among fish species. This study represents the first linkage map in the turbot, one of the most important flatfish in European aquaculture. This map will be suitable for QTL identification of productive traits in this species and for further evolutionary studies in fish and vertebrate species.
authors: Department of Genetics, University of Santiago de Compostela, Lugo, Spain.
authors: Carmen
authors: C
authors: Bouza
authors: Miguel
authors: M
authors: Hermida
authors: Belén G
authors: BG
authors: Pardo
authors: Carlos
authors: C
authors: Fernández
authors: Gloria G
authors: GG
authors: Fortes
authors: Jaime
authors: J
authors: Castro
authors: Laura
authors: L
authors: Sánchez
authors: Pablo
authors: P
authors: Presa
authors: Montse
authors: M
authors: Pérez
authors: Andrés
authors: A
authors: Sanjuán
authors: Alejandro
authors: A
authors: de Carlos
authors: José Antonio
authors: JA
authors: Alvarez-Dios
authors: Susana
authors: S
authors: Ezcurra
authors: Rosa M
authors: RM
authors: Cal
authors: Francesc
authors: F
authors: Piferrer
authors: Paulino
authors: P
authors: Martínez
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D005260:Female; D005412:Flatfishes / Q000235:genetics*; D008040:Genetic Linkage; D016678:Genome; D008297:Male; D018895:Microsatellite Repeats; D011995:Recombination, Genetic; D026801:Synteny