title: Time courses of growth and remodeling of porcine aortic media during hypertension: a quantitative immunohistochemical examination.
abstract: Arteries undergo marked structural and functional changes in human and experimental hypertension that generally involve smooth muscle cell (SMC) hypertrophy/hyperplasia as well as abnormal extracellular matrix turnover. In this study we examined time courses of changes in SMC activity and matrix protein content in a novel mini-pig aortic coarctation model. Cell proliferation was evaluated by immunostaining of Ki-67, apoptosis was assessed by TUNEL, and phenotypic changes were monitored by immunostaining three SMC contractile markers (caldesmon, calponin, and smoothelin). Changes in medial collagen and elastin were examined by picrosirius red and Verhoeff-van Gieson staining, respectively. LabVIEW-based image analysis routines were developed to objectively and efficiently quantify the (immuno)histochemical results. We found that significant cell proliferation and matrix production occurred in the early stages of this coarctation model and then declined gradually; the SMCs also tended to exhibit a less contractile phenotype following these cellular and extracellular changes. Specifically, different aspects of the phenotypic changes associated with hypertension occurred at different rates: cell proliferation and collagen production occurred early and peaked by 2 weeks, whereas changes in contractile protein expression continued to decrease over the entire 8-week study period. Temporal changes found in this study emphasize the importance of simultaneously tracing time courses of SMC growth and differentiation as well as matrix protein production and content. SMCs are multifunctional, and caution must be used to not overdefine phenotype. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.
authors: Department of Systems Biology and Translational Medicine, Texas A&M Health Science Center, Reynolds Medical Building, 1114 TAMU, College Station, TX 77843-1114, USA.
authors: Jin-Jia
authors: JJ
authors: Hu
authors: Andy
authors: A
authors: Ambrus
authors: Theresa W
authors: TW
authors: Fossum
authors: Matthew W
authors: MW
authors: Miller
authors: Jay D
authors: JD
authors: Humphrey
authors: Emily
authors: E
authors: Wilson
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000473:pathology*; D017209:Apoptosis; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000378:metabolism; D049109:Cell Proliferation; D003094:Collagen / Q000378:metabolism; D003627:Data Interpretation, Statistical; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006973:Hypertension / Q000378:metabolism / Q000473:pathology*; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D019394:Ki-67 Antigen / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000473:pathology*; D012984:Software; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors; D000096985:Calponins
chemical_list: D000199:Actins; D002135:Calcium-Binding Proteins; D002148:Calmodulin-Binding Proteins; D003094:Collagen; D016326:Extracellular Matrix Proteins; D019394:Ki-67 Antigen; D008840:Microfilament Proteins; D009124:Muscle Proteins; D000096985:Calponins