title: Neuroendocrine secretory protein 55 (NESP55) in the spinal cord of rat: an immunocytochemical study.
abstract: The immunohistochemical expression of a novel chromogranin-like protein, neuroendocrine secretory protein 55 (NESP55), in the rat spinal cord was investigated. NESP55-immunoreactive cells were detected in the ventral horn, intermediate laminae, and deep dorsal horn, comprising motoneurons, autonomic neurons, and interneurons throughout all spinal segments. Within laminae I-II of the dorsal horn, one or two NESP55-positive cells were often seen. Nerve fibers also contained NESP55 immunoreactivity (IR) and were particularly prominent in the ventral horn. No nerve terminals/varicosities appeared to contain NESP55 in any spinal lamina. Double-staining experiments revealed that a high proportion of the NESP55-positive neurons were cholinergic. Moreover, NESP55-IR in the motoneurons was evenly distributed in the whole cytoplasm with a finely granular appearance. In contrast, the fluorescent material in the preganglionic neurons was concentrated in the perinuclear region and largely overlapped with the trans-Golgi network marker TGN38. Our data provide detailed morphological information on the distribution of NESP55-IR in the rat spinal cord. Also, the differential intracellular expression of NESP55-IR in the spinal motoneurons and autonomic neurons suggests that NESP55 may be processed into different secretory granules and may be involved in both constitutive and regulated pathways in these neurons.
authors: Department of Anatomy and Cell Biology, Institute of Biomedicine, Göteborg University, SE-405 30 Göteborg, Sweden. yongling.li@anatcell.gu.se
authors: Yongling
authors: Y
authors: Li
authors: Reiner
authors: R
authors: Fischer-Colbrie
authors: Annica
authors: A
authors: Dahlström
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D001341:Autonomic Nervous System / Q000166:cytology / Q000378:metabolism; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D002864:Chromogranins; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000378:metabolism*; D007150:Immunohistochemistry; D007395:Interneurons / Q000166:cytology / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009489:Neurosecretion / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D022142:Secretory Vesicles / Q000378:metabolism / Q000648:ultrastructure; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*
chemical_list: D002864:Chromogranins; D008562:Membrane Glycoproteins; C513045:Tgoln2 protein, rat; C498643:Gnas protein, rat; D019205:GTP-Binding Protein alpha Subunits, Gs; D000109:Acetylcholine