title: Effects of the G376E and G157D mutations on the stability of yeast enolase--a model for human muscle enolase deficiency.
abstract: The first known human enolase deficiency was reported in 2001 [Comi GP, Fortunato F, Lucchiari S, Bordoni A, Prelle A, Jann S, Keller A, Ciscato P, Galbiati S, Chiveri L et al. (2001) Ann Neurol50, 202-207]. The subject had inherited two mutated genes for beta-enolase. These mutations changed glycine 156 to aspartate and glycine 374 to glutamate. In order to study the effects of these changes on the structure and stability of enolase, we have introduced the corresponding changes (G157D and G376E) into yeast enolase. The two variants are correctly folded. They are less stable than wild-type enolase with respect to thermal denaturation, and both have increased Kd values for subunit dissociation. At 37 degrees C, in the presence of salt, both are partially dissociated and are extensively cleaved by trypsin. Under the same conditions, wild-type enolase is fully dimeric and is only slightly cleaved by trypsin. However, wild-type enolase is also extensively cleaved if it is partially dissociated. The identification of the cleavage sites and spectral studies of enolase have revealed some of the structural differences between the dimeric and monomeric forms of this enzyme.
authors: Department of Chemistry and Biochemistry, Concordia University, Montreal, Canada.
authors: Songping
authors: S
authors: Zhao
authors: Bonny S F
authors: BS
authors: Choy
authors: Mary J
authors: MJ
authors: Kornblatt
mesh_terms: D002942:Circular Dichroism; D004795:Enzyme Stability; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D018482:Muscle, Skeletal / Q000201:enzymology; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D010751:Phosphopyruvate Hydratase / Q000737:chemistry* / Q000172:deficiency / Q000235:genetics*; D011487:Protein Conformation; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013329:Structure-Activity Relationship; D013696:Temperature
chemical_list: D010751:Phosphopyruvate Hydratase