Bent oligonucleotide duplexes as HMGB1 inhibitors: a comparative study.
In this work we explore the ability of a chimeric LNA/DNA bent duplex, in which the kink is induced by 2 unpaired adenines in the middle of one strand, to bind HMGB1, a protein involved in many inflammatory processes. The LNA/DNA duplex was compared with the corresponding full DNA and PNA/DNA chimera duplexes from a thermodynamic and spectroscopic point of view.
Musumeci
D
D
Istituto di Biostutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Naples, Italy. domymusu@alice.it
Roviello
Giovanni N
GN
Moccia
M
M
Pedone
C
C
Bucci
E M
EM
Sapio
R
R
Valente
M
M
Fumero
S
S
D004247:DNA / Q000737:chemistry* / Q000494:pharmacology*; D024243:HMGB1 Protein / Q000037:antagonists & inhibitors*; D006801:Humans; D009841:Oligonucleotides / Q000737:chemistry* / Q000494:pharmacology*
D024243:HMGB1 Protein; D009841:Oligonucleotides; C477371:locked nucleic acid; D004247:DNA