title: Anti-proliferate and pro-apoptotic effects of 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyranone through inactivation of NF-kappaB in human colon cancer cells.
abstract: Many natural compounds have been shown to prevent cancer cell growth through the redox regulation of transcription factors. NF-kappaB, a redox transcription factor, has been implicated in the apoptotic cell death of several cancer cells. This study examined whether or nor 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyranone (DDMP) isolated from onions can modulate the activity of NF-kappaB, thereby induce the apoptotic cell death of colon cancer cells. Treatment with different DDMP concentrations (0.5-1.5 mg/mL) for various periods (0-48 h) inhibited the growth of colon cancer cells (SW620 and HCT116) followed by the induction of apoptosis in a dose dependent manner. It was also found that DDMP modulated tumor necrosis factor-alpha (TNF-alpha) and tetradeanoyl phorbol acetate (TPA)-induced NF-kappaB transcriptional and DNA binding activity. Moreover, DDMP suppressed the NF-kappaB target anti-apoptotic genes (Bcl-2), whereas it induced the expression of the apoptotic genes (Bax, cleaved caspase-3 and cleaved PARP). These results suggest that DDMP from onions inhibit colon cancer cell growth by inducing apoptotic cell death through the inhibition of NF-kappaB.
authors: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk, Korea.
authors: Jung Ok
authors: JO
authors: Ban
authors: In Guk
authors: IG
authors: Hwang
authors: Tae Myoung
authors: TM
authors: Kim
authors: Bang Yeon
authors: BY
authors: Hwang
authors: Ung Soo
authors: US
authors: Lee
authors: Heon-Sang
authors: HS
authors: Jeong
authors: Young Won
authors: YW
authors: Yoon
authors: Dae Joong
authors: DJ
authors: Kimz
authors: Jin Tae
authors: JT
authors: Hong
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D012503:Saponins / Q000494:pharmacology*; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: C427244:2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one; D000970:Antineoplastic Agents; D016328:NF-kappa B; D012503:Saponins; D014315:Triterpenes