Biological scaffolds for heart valve tissue engineering.
Tissue engineering might bear promising solutions for overcoming the limitations of biological and mechanical heart valve substitutes. The concept of heart valve tissue engineering bases on decellularized biological matrices, as removal of cellular components might reduce immunological reactions, which are thought to be responsible for accelerated valvular graft deterioration and their subsequent repopulation with autologous cells, which leads to tissue integrity and continuous remodeling. Here, we report a method for efficient removal of cells from ovine heart valve tissue (including cusp, wall, and myocardial cuff) by detergents for the generation of biological valvular scaffolds.
Lichtenberg
Artur
A
Department of Thoracic and Cardiovascular Surgery, Hanover Medical School, Germany.
Cebotari
Serghei
S
Tudorache
Igor
I
Hilfiker
Andres
A
Haverich
Axel
A
D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D001705:Bioprosthesis; D006321:Heart / Q000033:anatomy & histology / Q000502:physiology; D006350:Heart Valve Prosthesis; D006351:Heart Valves / Q000033:anatomy & histology*; D006801:Humans; D011474:Prosthesis Design; D051381:Rats; D012756:Sheep; D023822:Tissue Engineering
D001672:Biocompatible Materials