Chicken collagen type II reduces articular cartilage destruction in a model of osteoarthritis in rats.
OBJECTIVE
To evaluate the therapeutic effects of domestic chicken collagen type II (CCII) on rat osteoarthritis (OA) and analyze concomitant changes in the level of Matrix metalloproteinase (MMP)-13, MMP-9, Cathepsin K and their mRNA as well as the tissue inhibitor of matrix metalloproteinase (TIMP)-1 mRNA in articular cartilage of osteoarthritic rats.


METHODS
Osteoarthritis models were surgically induced. Morphology of articular cartilage was done by haematoxylin and eosin staining and Mankin score was calculated, immunohistochemistry of MMP-13, MMP-9 and Cathepsin K was done by ABC method while the mRNA level for MMP-13, MMP-9, cathepsin K as well as TIMP-1 was evaluated by RT-PCR method.


RESULTS
Oral administration of CCII reduced the morphological changes of osteoarthritic cartilage (shown by Mankin score), decreased levels of MMP-13, MMP-9, cathepsin K as well as their mRNA in articular cartilage from osteoarthritic rats while it exhibited no effect on TIMP-1 mRNA.


CONCLUSIONS
Oral CCII reduced articular cartilage degradation of osteoarthritic rats and may probably be a potent drug candidate for OA treatment.
Xu
D
D
Clinical Pharmacology Institute, Shanghai Sixth Municipal Hospital, Shanghai Jiao Tong University, Shanghai 200233, China. xdh@sjtu.edu.cn
Shen
W
W
D000818:Animals; D002358:Cartilage, Articular / Q000473:pathology*; D056657:Cathepsin K; D002403:Cathepsins / Q000187:drug effects; D002645:Chickens; D024043:Collagen Type II / Q000627:therapeutic use*; D006305:Health Status Indicators; D007150:Immunohistochemistry; D053509:Matrix Metalloproteinase 13 / Q000187:drug effects; D020780:Matrix Metalloproteinase 9 / Q000187:drug effects; D023421:Models, Animal; D010003:Osteoarthritis / Q000188:drug therapy* / Q000503:physiopathology; D012333:RNA, Messenger; D051381:Rats; D017208:Rats, Wistar; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000187:drug effects
D024043:Collagen Type II; D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D002403:Cathepsins; D056657:Cathepsin K; C534133:Ctsk protein, rat; D053509:Matrix Metalloproteinase 13; D020780:Matrix Metalloproteinase 9