Vicinal diaminobenzoacridine used as the fluorescent probe for trace nitric oxide determination by flow injection spectrofluorimetry and macrophage cells imaging.
Based on a photoelectron transfer (PET) mechanism, vicinal diaminobenzoacridine (VDABA), a fluorescent probe for the determination of trace amounts of nitric oxide radical in biological sample, was synthesized and characterized by elemental analysis, IR, (1)H NMR and (13)C NMR spectrum. Combining a flow injection with spectrofluorimetry, a high-throughput method for detecting NO was obtained, which was successfully applied to the determination of NO in the human serum. The proposed method was simple, rapid, precise and automatic. Under optimum conditions, the linear calibration range was from 1.1 x 10(-7) to 5.0 x 10(-6)M and the detection limit was 3.1 x 10(-8)M. Furthermore, the probe could make cell-derived NO "visible" by using confocal laser scanning microscope.
Hu
Jixi
J
College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Shandong Normal University, Jinan 250014, PR China.
Yin
Lingling
L
Xu
Kehua
K
Gao
Jingjing
J
Tong
Lili
L
Tang
Bo
B
D000166:Acridines / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis*; D006801:Humans; D006851:Hydrochloric Acid; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000032:analysis*; D017382:Reactive Oxygen Species / Q000737:chemistry; D012984:Software; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013696:Temperature
D000166:Acridines; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; C526148:vicinal diaminobenzoacridine; D009569:Nitric Oxide; D006851:Hydrochloric Acid