The usefulness of CD26 in flow cytometric analysis of peripheral blood in Sézary syndrome.
The loss of CD26 expression was proposed to be a constant feature of circulating Sézary cells by flow cytometric immunophenotyping (FCIP), but the experience with CD26 is limited. To establish its usefulness, CD26 results were correlated with morphologic, molecular, and immunophenotypic findings. Based on FCIP of 179 samples of peripheral blood, CD26 negativity was found in 59.3% of cases with Sézary syndrome (SS), 33.3% of mycosis fungoides (MF), 14.2% of benign dermatosis (BD), and no control cases. In diagnostic subgroups of SS based on morphologic, molecular, and immunophenotypic criteria, the percentage of CD26- cases varied from 41.1% to 63.6%. The specificity of a CD26- result was inferior to that of T-cell antigen loss in differentiating SS from MF and BD. CD26 offers lower diagnostic performance than previously suggested; however, in addition to the findings of major T-cell antigen loss, it could improve sensitivity of FCIP in patients with SS.
Kelemen
Katalin
K
Department of Pathology, Northwestern Memorial Hospital, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
Guitart
Joan
J
Kuzel
Timothy M
TM
Goolsby
Charles L
CL
Peterson
Loann C
LC
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis; D003937:Diagnosis, Differential; D018819:Dipeptidyl Peptidase 4 / Q000097:blood*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D009182:Mycosis Fungoides / Q000097:blood / Q000175:diagnosis; D012680:Sensitivity and Specificity; D012751:Sezary Syndrome / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D012871:Skin Diseases / Q000097:blood / Q000175:diagnosis; D012878:Skin Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4