Hybrid photoacoustic fluorescence molecular tomography using finite-element-based inversion.
Improvements in fluorescence reconstruction when utilizing a hybrid photoacoustic (PAT) fluorescence molecular tomography (FMT) method to image optically heterogeneous media are studied and showcased. Quantitative optical absorption maps are retrieved using a normalized backprojection algorithm for PAT reconstruction. Consecutively, the reconstructed absorption distribution is employed into computing a diffusion-equation-based forward model for FMT using a finite-element solution. The potential promise of the suggested method is experimentally verified on tissue-mimicking fluorescent phantoms, where improvements in the quality of FMT reconstructions are observed when imaging at the presence of a large absorber.
Razansky
Daniel
D
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA. daniel.razansky@gmail.com
Ntziachristos
Vasilis
V
D000042:Absorption; D000162:Acoustics; D000465:Algorithms; D002138:Calibration; D004058:Diffusion; D004867:Equipment Design; D020342:Finite Element Analysis; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007834:Lasers; D015233:Models, Statistical; D019047:Phantoms, Imaging; D041622:Tomography, Optical / Q000379:methods*; D014057:Tomography, X-Ray Computed / Q000379:methods*