Intra- and intermolecular interactions influence the reactivity of RNA oligonucleotides.
The transesterification of RNA oligonucleotides was studied over a wide pH range. The rate constants obtained indicate that, under neutral conditions, oligonucleotides with an adenosine moiety as the 5'-linked nucleoside can be up to 1000-fold more reactive than the reference oligonucleotide, a 13-mer oligo-U (1). Experiments with the modified oligonucleotide with N6,N6-dimethyladenosine (9) as the 5'-linked nucleoside moiety suggest that the exocyclic amino group is involved in the reaction, influencing the reactivity of the neighboring phosphodiester bond. In addition to such intramolecular interactions, weak intermolecular interactions most probably contribute to the reactivity.
Linjalahti
Heidi
H
University of Turku, Department of Chemistry, FIN-20014, Turku.
Mikkola
Satu
S
D001483:Base Sequence; D004952:Esters / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009841:Oligonucleotides / Q000737:chemistry*; D010766:Phosphorylation; D012313:RNA / Q000737:chemistry*
D004952:Esters; D009841:Oligonucleotides; D012313:RNA