title: Development of a flow-through system to create occluding thrombus.
abstract: Occlusive thrombosis accounts for many heart attacks and strokes. These acute events are difficult to catch in patients and animal test methods may be misleading because anti-thrombotic therapeutics often do not cross-react with different species. This paper presents a new flow-through system that leads to rapid occlusive thrombosis in arterial flow conditions. Whole porcine blood is perfused through a tubular test section. The growing thrombus is visualized in real time from early platelet attachment, through accumulation, to occlusion. The progression of flow rate reduction provides a clear distinguishing parameter between thrombus formation and embolization. Thrombus growth rate is a linear function of very high shear rate beyond 40,000 s(-1). The histology of the thrombus reveals predominantly platelet accumulation and growth as a rough surface with tendrils. This flow-through system may be useful for the economic testing of new anti-thrombosis therapies.
authors: GWW School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332-0405, USA. david.ku@me.gatech.edu
authors: David N
authors: DN
authors: Ku
authors: Conor J
authors: CJ
authors: Flannery
mesh_terms: D000818:Animals; D005337:Fibrin / Q000378:metabolism; D018056:Hemorheology; D008955:Models, Cardiovascular; D010973:Platelet Adhesiveness; D010974:Platelet Aggregation; D034421:Sus scrofa; D013927:Thrombosis / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D014841:von Willebrand Factor; D005337:Fibrin