Molecular characterization of the first polyomavirus from a New World primate: squirrel monkey polyomavirus.
DNA samples from a variety of New World monkeys were screened by using a broad-spectrum PCR targeting the VP1 gene of polyomaviruses. This resulted in the characterization of the first polyomavirus from a New World primate. This virus naturally infects squirrel monkeys (Saimiri sp.) and is provisionally named squirrel monkey polyomavirus (SquiPyV). The complete genome of SquiPyV is 5,075 bp in length, and encodes the small T and large T antigens and the three structural proteins VP1, VP2 and VP3. Interestingly, the late region also encodes a putative agnoprotein, a feature that it shares with other polyomaviruses from humans, baboons and African green monkeys. Comparison with other polyomaviruses revealed limited sequence similarity to any other polyomavirus, and phylogenetic analysis of the VP1 gene confirmed its uniqueness.
Verschoor
Ernst J
EJ
Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
Groenewoud
Marlous J
MJ
Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
Fagrouch
Zahra
Z
Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
Kewalapat
Aruna
A
Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
van Gessel
Sabine
S
Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
Kik
Marja J L
MJL
Department of Pathobiology, Pathology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Heeney
Jonathan L
JL
Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005784:Gene Amplification; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D011120:Polyomavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012453:Saimiri / Q000821:virology*
D036022:Capsid Proteins; D004279:DNA, Viral