[Investigation of toxigenic microcystis and microcystin pollution in Huayuankou Conservation Pool of Yellow River].
OBJECTIVE
To investigate the contaminative, condition of planktonic algae, cyanobacteria, toxigenic microcystis and microcystin in Huayuankou Conservation Pool of Yellow River.


METHODS
From March 2005 to January 2006, water samples were taken 15 times by 2. 5L plastic sampler from Huayuankou Conservation Pool. The density of algae were counted by using blood cell counter. Phycocyanin intergenic spacer region (PC-IGS) and microcystin synthetase gene B (mcyB) of toxigenic microcystis was identified by the whole cell PCR. The concentration of microcystin was determined by ELISA kit. The positive results of PCR and ELISA were compared.


RESULTS
Bacillariophyta, chlorophyta, cyanophyta (cyanobacteria) and euglenophyta were main algaes in Huayuankou conservation pool, and the dominant algae and cell density changed seasonally. Algae cell density and cyanobacteria cell density were higher in summer and autumn than in spring and winter. From July to November, 2005, PC-IGS and mcyB were detected positively by whole cell PCR. Microcystin was positively detected from July, the concentration of microcystin changed from 0 to 0.25microg/L, it was more higher in summer than other seasons.


CONCLUSIONS
Toxigenic microcystis and microcystin could be detected in Huayuankou Conservation Pool of Yellow River. Whole cell PCR could be used to identify toxigenic microcystis.
Ban
Haiqun
H
School of Public Health, Zhengzhou University, Zhengzhou 450052, China.
Ba
Yue
Y
Cheng
Xuemin
X
Wang
Guangzhou
G
D002681:China; D000460:Chlorophyta / Q000254:growth & development / Q000378:metabolism; D017377:Diatoms / Q000254:growth & development / Q000378:metabolism; D004784:Environmental Monitoring; D052998:Microcystins / Q000032:analysis*; D046931:Microcystis / Q000254:growth & development* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D014876:Water Pollution / Q000032:analysis*; D014881:Water Supply / Q000032:analysis*
D052998:Microcystins; C078588:microcystin