Profiling infection responses in the haemocytes of the mosquito, Aedes aegypti.
Pathogens that infect and/or are transmitted by mosquitoes typically are exposed to the body cavity, and to haemocytes circulating therein, during development or dissemination. Aedes aegypti haemocytes produce a range of immune response-related gene products, and an endpoint response of phagocytosis and/or melanization that is temporally and structurally distinct for the invading pathogen. Expressed sequence tags were generated from haemocyte libraries and then used to design oligonucleotide microarrays. Arrays were screened with haemocyte material collected 1-, 8- and 24-h post-inoculation with Escherichia coli or Micrococcus luteus bacteria. Data from these studies support the discovery of novel immune response-activated genes, provide an expanded understanding of antimicrobial peptide biology and highlight the coordination of immune factors that leads to an endpoint response.
Bartholomay
L C
LC
Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI, USA.
Mayhew
G F
GF
Fuchs
J F
JF
Rocheleau
T A
TA
Erickson
S M
SM
Aliota
M T
MT
Christensen
B M
BM
D000330:Aedes / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D017344:Genes, Insect; D006434:Hemocytes / Q000276:immunology / Q000382:microbiology; D019476:Insect Proteins / Q000235:genetics / Q000276:immunology; D008543:Melanins / Q000235:genetics; D016982:Micrococcus luteus / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis
D023181:Antimicrobial Cationic Peptides; D019476:Insect Proteins; D008543:Melanins