title: Laparoscopic appendectomy without clip or ligature. An experimental study.
abstract: BACKGROUND
  We aimed to test the efficacy and safety of closure of the appendeceal stump with only laparoscopic bipolar electrocautery in rats.
  
  
  METHODS
  In this study, 40 female Wistar-Albino rats were used. In group I (n = 10), appendix vermiformis, approximately 1 cm in width, was completely ligated with 3/0 silk suture close to cecum, and removed. In group II (n = 20) and group III (n = 10), the appendeceal stump was coagulated by bipolar cautery. The coagulation of 70 mA took 10 s, and was repeated one more time. The stump was divided, and checked to ensure complete occlusion. Groups I and II underwent relaparotomy at 15 days, cecum was taken out, and the burst pressure of the stump was measured. Group III did not undergo relaparotomy; the burst pressure was measured during the first laparotomy.
  
  
  RESULTS
  All rats survived. At relaparotomy, no intra-abdominal complications were detected, including intestinal obstruction, abscess, and leakage. Omentum and fatty tissue of uterus was adhered to the appendix stump in group I, but only fatty tissue of uterus was adhered on the stump in group II. Although the intracecal pressure reached 30 cmH(2)O, at which pressure the cecum was highly stretched, ligated (group I) or coagulated (group II) stumps did not burst or opened. In group III, the burst or opening pressure of the stump (11.2 +/- 2.7 cmH(2)O) was significantly lower than in groups I and II (p < 0.001). Of group II rats, 80% had complete epithelial regeneration at the coagulated stump sites in contrast to ligated rats (p < 0.001) with severe inflammatory changes, abscess, and necrosis.
  
  
  CONCLUSIONS
  At late course, coagulated stumps did not allow the leakage or burst, unlike ligated stumps. However, coagulation of the stump seemed to contribute more to epithelial healing. This experimental model suggests that the closure of the stump with only bipolar coagulation was a safe and feasible method.
authors: Department of Pediatric Surgery, Akdeniz University School of Medicine Akdeniz Universitesi Tip Fakültesi, Cocuk Cerrahisi Anabilim Dali, 07070 Antalya, Turkey. adaslan@yahoo.com
authors: Adnan
authors: A
authors: Aslan
authors: Cagdas
authors: C
authors: Karaveli
authors: Ozlem
authors: O
authors: Elpek
mesh_terms: D000038:Abscess / Q000209:etiology; D000672:Amputation Stumps; D000818:Animals; D001062:Appendectomy / Q000295:instrumentation / Q000379:methods*; D002432:Cecum; D019245:Compressive Strength; D004564:Electrocoagulation / Q000295:instrumentation / Q000379:methods*; D005260:Female; D010535:Laparoscopy / Q000379:methods*; D008026:Ligation / Q000009:adverse effects; D011183:Postoperative Complications / Q000209:etiology; D051381:Rats; D017208:Rats, Wistar; D024162:Second-Look Surgery; D013314:Stress, Mechanical; D000267:Tissue Adhesions / Q000209:etiology / Q000473:pathology; D014945:Wound Healing