title: Expression of a novel 90-kDa protein, Lsd90, involved in the metabolism of very long-chain fatty acid-containing phospholipids in a mitosis-defective fission yeast mutant.
abstract: The fission yeast lsd1/fas2 strain carries a temperature-sensitive mutation of the fatty-acid-synthase alpha-subunit, exhibiting an aberrant mitosis lsd phenotype, with accumulation of very-long-chain fatty-acid-containing phospholipid (VLCFA-PL). A novel 90-kDa protein, Lsd90 (SPBC16E9.16c), was found to be newly expressed in small particle-like structures in lsd1/fas2 cells under restrictive conditions. Two mismatches leading to a double frame shift were found between the sequences of the lsd90(+) gene registered in the genomic database and the sequences determined experimentally at the amino acid, cDNA and genomic DNA levels. Unexpectedly, overexpression and disruption of the lsd90(+) gene in either lsd1/fas2 or wild-type cells did not affect either cell growth or expression of the lsd phenotype. The amounts of VLCFA-PL that accumulated in lsd90-overexpressing lsd1/fas2 cells were significantly lower than those in lsd1/fas2 cells, suggesting the involvement of Lsd90 in the metabolism of VLCFA-PL.
authors: Faculty of Pharmaceutical Sciences, Teikyo University, Suarashi, Sagamiko-machi, Sagamihara-shi, Kanagawa, Japan. yokoyama@pharm.teikyo-u.ac.jp
authors: Kazuaki
authors: K
authors: Yokoyama
authors: Miyuki
authors: M
authors: Nakagawa
authors: Masayuki
authors: M
authors: Satoh
authors: Shigeaki
authors: S
authors: Saitoh
authors: Naoshi
authors: N
authors: Dohmae
authors: Ayako
authors: A
authors: Harada
authors: Noriko
authors: N
authors: Satoh
authors: Ken
authors: K
authors: Karasawa
authors: Koji
authors: K
authors: Takio
authors: Mitsuhiro
authors: M
authors: Yanagida
authors: Keizo
authors: K
authors: Inoue
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005227:Fatty Acids / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D008938:Mitosis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D010743:Phospholipids / Q000378:metabolism*; D021381:Protein Transport; D020543:Proteome / Q000032:analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D010743:Phospholipids; D020543:Proteome; D012333:RNA, Messenger; D029702:Schizosaccharomyces pombe Proteins