Early manganese-toxicity response in Vigna unguiculata L.--a proteomic and transcriptomic study.
The apoplast is known to play a predominant role in the expression of manganese (Mn) toxicity in cowpea (Vigna unguiculata L.) leaves. To unravel early Mn-toxicity responses after 1-3 days Mn treatment also in the leaf symplast, we studied the symplastic reactions induced by Mn in two cultivars differing in Mn tolerance on a total cellular level. Comparative proteome analyses of plants exposed to low or high Mn allowed to identify proteins specifically affected by Mn, particularly in the Mn-sensitive cowpea cultivar. These proteins are involved in CO(2) fixation, stabilization of the Mn cluster of the photosystem II, pathogenesis-response reactions and protein degradation. Chloroplastic proteins important for CO(2) fixation and photosynthesis were of lower abundance upon Mn stress suggesting scavenging of metabolic energy for a specific stress response. Transcriptome analyses supported these findings, but additionally revealed an upregulation of genes involved in signal transduction only in the Mn-sensitive cultivar. In conclusion, a coordinated interplay of apoplastic and symplastic reactions seems to be important during the Mn-stress response in cowpea.
Führs
Hendrik
H
Institute for Plant Nutrition, Faculty of Natural Sciences, Leibniz University Hannover, Herrenhaeuser Strasse 2, Hannover, Germany.
Hartwig
Moritz
M
Molina
Laura Elisa Buitrago
LE
Heintz
Dimitri
D
Van Dorsselaer
Alain
A
Braun
Hans-Peter
HP
Horst
Walter J
WJ
D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D007887:Fabaceae / Q000737:chemistry* / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D008345:Manganese / Q000502:physiology / Q000633:toxicity*; D008969:Molecular Sequence Data; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology; D045332:Photosystem II Protein Complex / Q000378:metabolism; D010935:Plant Diseases / Q000139:chemically induced / Q000235:genetics; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics; D020543:Proteome / Q000235:genetics
D045332:Photosystem II Protein Complex; D020543:Proteome; D008345:Manganese