Ischemically damaged heart after preservation by the cavitary two-layer method as a possible donor in rat heart transplantation.
BACKGROUND
The purpose of this study was to examine the possibility of using an ischemically damaged heart, after preservation by the cavitary two-layer (CTL) method, as a donor in heart transplantation.


METHODS
Each donor heart was heterotopically transplanted to the recipient aorta. The grafts in Group 1 were immediately transplanted. In Group 2, the grafts with (a) 15- or (b) 30-minute warm ischemia were transplanted. The ischemically damaged grafts were transplanted after preservation for 3 hours in University of Wisconsin (UW) solution (Group 3) or CTL (Group 4). Five-day animal survival, tissue adenine triphosphate (ATP) concentration, biochemical assay and histopathologic data were obtained.


RESULTS
Five-day survival in Group 4a was 7 of 8, with significant recovery of the ATP tissue level (9.31 +/- 0.80 micromol/dry weight). Biochemical and pathologic examinations demonstrated that ischemia-reperfusion injury was prevented in Group 4a compared with Group 2a.


CONCLUSIONS
An ischemically damaged rat heart preserved for 3 hours by CTL was found to be a potential donor in rat heart transplantation.
Kamoda
Yasuhisa
Y
Department of Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
Fujino
Yasuhiro
Y
Tanioka
Yasuki
Y
Sakai
Tetsuya
T
Kuroda
Yoshikazu
Y
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D003080:Cold Temperature; D006321:Heart / Q000502:physiology; D016027:Heart Transplantation / Q000473:pathology / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000378:metabolism; D008297:Male; D017202:Myocardial Ischemia / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000517:prevention & control; D016019:Survival Analysis; D052096:Warm Ischemia / Q000009:adverse effects
D000227:Adenine Nucleotides; D006360:Heat-Shock Proteins