title: Adipose gene expression prior to weight loss can differentiate and weakly predict dietary responders.
abstract: BACKGROUND
  The ability to identify obese individuals who will successfully lose weight in response to dietary intervention will revolutionize disease management. Therefore, we asked whether it is possible to identify subjects who will lose weight during dietary intervention using only a single gene expression snapshot.
  
  
  RESULTS
  The present study involved 54 female subjects from the Nutrient-Gene Interactions in Human Obesity-Implications for Dietary Guidelines (NUGENOB) trial to determine whether subcutaneous adipose tissue gene expression could be used to predict weight loss prior to the 10-week consumption of a low-fat hypocaloric diet. Using several statistical tests revealed that the gene expression profiles of responders (8-12 kgs weight loss) could always be differentiated from non-responders (<4 kgs weight loss). We also assessed whether this differentiation was sufficient for prediction. Using a bottom-up (i.e. black-box) approach, standard class prediction algorithms were able to predict dietary responders with up to 61.1%+/-8.1% accuracy. Using a top-down approach (i.e. using differentially expressed genes to build a classifier) improved prediction accuracy to 80.9%+/-2.2%.
  
  
  CONCLUSIONS
  Adipose gene expression profiling prior to the consumption of a low-fat diet is able to differentiate responders from non-responders as well as serve as a weak predictor of subjects destined to lose weight. While the degree of prediction accuracy currently achieved with a gene expression snapshot is perhaps insufficient for clinical use, this work reveals that the comprehensive molecular signature of adipose tissue paves the way for the future of personalized nutrition.
authors: INSERM, Nutriomique U872, Paris, France.
authors: David M
authors: DM
authors: Mutch
authors: M Ramzi
authors: MR
authors: Temanni
authors: Corneliu
authors: C
authors: Henegar
authors: Florence
authors: F
authors: Combes
authors: Véronique
authors: V
authors: Pelloux
authors: Claus
authors: C
authors: Holst
authors: Thorkild I A
authors: TI
authors: Sørensen
authors: Arne
authors: A
authors: Astrup
authors: J Alfredo
authors: JA
authors: Martinez
authors: Wim H M
authors: WH
authors: Saris
authors: Nathalie
authors: N
authors: Viguerie
authors: Dominique
authors: D
authors: Langin
authors: Jean-Daniel
authors: JD
authors: Zucker
authors: Karine
authors: K
authors: Clément
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000465:Algorithms; D004032:Diet; D002149:Energy Intake; D005260:Female; D015870:Gene Expression; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015431:Weight Loss