title: Differential effects of black raspberry and strawberry extracts on BaPDE-induced activation of transcription factors and their target genes.
abstract: The chemopreventive properties of edible berries have been demonstrated both in vitro and in vivo, however, the specific molecular mechanisms underlying their anti-cancer effects are largely unknown. Our previous studies have shown that a methanol extract fraction of freeze-dried black raspberries inhibits benzoapyrene (BaP)-induced transformation of Syrian hamster embryo cells. This fraction also blocks activation of activator protein-1 (AP-1) and nuclear factor kappaB (NF-kappaB) induced by benzoapyrene diol-epoxide (BaPDE) in mouse epidermal JB6 Cl 41 cells. To determine if different berry types exhibit specific mechanisms for their anti-cancer effects, we compared the effects of extract fractions from both black raspberries and strawberries on BaPDE-induced activation of various signaling pathways in Cl 41 cells. Black raspberry fractions inhibited the activation of AP-1, NF-kappaB, and nuclear factor of activated T cells (NFAT) by BaPDE as well as their upstream PI-3K/Akt-p70(S6K) and mitogen-activated protein kinase pathways. In contrast, strawberry fractions inhibited NFAT activation, but did not inhibit the activation of AP-1, NF-kappaB or the PI-3K/Akt-p70(S6K) and mitogen-activated protein kinase pathways. Consistent with the effects on NFAT activation, tumor necrosis factor-alpha (TNF-alpha) induction by BaPDE was blocked by extract fractions of both black raspberries and strawberries, whereas vascular endothelial growth factor (VEGF) expression, which depends on AP-1 activation, was suppressed by black raspberry fractions but not strawberry fractions. These results suggest that black raspberry and strawberry components may target different signaling pathways in exerting their anti-carcinogenic effects.
authors: Nelson Institute of Environmental Medicine, New York University School of Medicine, New York, New York 10987, USA.
authors: Jingxia
authors: J
authors: Li
authors: Dongyun
authors: D
authors: Zhang
authors: Gary D
authors: GD
authors: Stoner
authors: Chuanshu
authors: C
authors: Huang
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000494:pharmacology*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000737:chemistry; D002273:Carcinogens / Q000494:pharmacology; D002460:Cell Line; D006224:Cricetinae; D031985:Fragaria / Q000737:chemistry / Q000502:physiology; D005638:Fruit / Q000737:chemistry / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D051379:Mice; D016328:NF-kappa B / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018808:Transcription Factor AP-1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D016588:Anticarcinogenic Agents; D002273:Carcinogens; D016328:NF-kappa B; D010936:Plant Extracts; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide