Xenopus oocyte wound healing as a model system for analysis of microtubule-actin interactions.
Microtubule-actin interactions are fundamental to many cellular processes such as cytokinesis and cellular locomotion. Investigating the mechanism of microtubule-actin interactions is the key to understand the cellular morphogenesis and related pathological processes. The abundance and highly dynamic nature of microtubules and F-actin raise a serious challenge when trying to distinguish between the real and fortuitous interactions within a cell. Xenopus oocyte wound model represents an ideal system to study microtubule-actin interactions as well as microtubule-dependent control of the actin polymerization. Here, we describe a series of cytoskeleton specific treatments in Xenopus oocyte wound healing experiments and use confocal fluorescence microscopy to analyze fixed oocytes to examine microtubule-actin interactions.
Zhang
Tong
T
McGill University, Montreal, Canada.
Mandato
Craig A
CA
D000199:Actins / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D005260:Female; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D008870:Microtubules / Q000737:chemistry* / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D017239:Paclitaxel / Q000737:chemistry; D014945:Wound Healing / Q000502:physiology*; D014982:Xenopus laevis
D000199:Actins; D017239:Paclitaxel