[Identification of a functional ITAM-like sequence within G1 cytoplasmic tail of Hantaan virus].
The G1 cytoplasmic tail of Hantaan virus (HTNV) harbors a highly conserved region, which is homologous to immunoreceptor tyrosine-based activation motifs (ITAM) and is termed the ITAM-like sequence. To demonstrate the potential signal-transducing activity of G1 ITAM-like sequence resembling the canonical ITAM within immune and endothelial cells, a series of experiments were performed to define its interaction with cellular kinases. The synthesized G1 ITAM-like peptide was shown to coprecipitate with cellular phosphoprotein complexes by an immune-complex kinase assay. Mutational analyses showed that this ITAM-like sequence was a substrate for the Src family kinase Fyn, and two conserved tyrosine residues were required for coprecipitating Lyn, Syk, and ZAP-70 kinases. These findings demonstrated that HTNV envelope glycoprotein G1 contains a functional ITAM-like sequence in its cytoplasmic tail, which can bind critical cellular kinases that regulate immune and endothelial cell functions.
Mou
Dan-Lei
DL
Center of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. moudanle@yahoo.com.cn
Wang
Ying-Peng
YP
Jiang
Hong
H
Xiao
Shu-Yuan
SY
Yu
Xu
X
Li
Guang-Yu
GY
Wang
Ping-Zhong
PZ
Sun
Yong-Tao
YT
Nie
Qing-He
QH
Huang
Chang-Xing
CX
Bai
Xue-Fan
XF
D000595:Amino Acid Sequence; D002478:Cells, Cultured; D018063:Hantaan virus / Q000737:chemistry* / Q000502:physiology; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000502:physiology; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000502:physiology; D051076:Proto-Oncogene Proteins c-fyn / Q000502:physiology; D015398:Signal Transduction; D000072377:Syk Kinase; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000502:physiology
D047908:Intracellular Signaling Peptides and Proteins; D014759:Viral Envelope Proteins; C104008:glycoprotein G1, Hantavirus; D011505:Protein-Tyrosine Kinases; D051076:Proto-Oncogene Proteins c-fyn; C000606896:SYK protein, human; D000072377:Syk Kinase