title: [Cloning of human bone morphogenetic protein-2 gene and the construction of its eukaryotic expression vector].
abstract: OBJECTIVE
  To clone human bone morphogenetic protein-2 (hBMP2) gene and construct its eukaryotic expression vector pcDNA3.1 -hBMP2.
  
  
  METHODS
  Human BMP2 gene was amplified by RT-PCR method from human osteosarcoma cells and constructed into eukaryotic expression vector pcDNA3.1-hBMP2. The gene in the vector pcDNA3.1-hBMP2 was identified by PCR amplification, enzyme digestion and DNA sequencing.
  
  
  RESULTS
  The cloned DNA was confirmed to be hBMP-2 gene.
  
  
  CONCLUSIONS
  In this study, hBMP2 gene is successfully cloned and its eukaryotic expression vector pcDNA3.1-hBMP2 is constructed, which provides the foundation of using BMP2 gene therapy to accelerate new bone formation in distraction osteogenesis.
authors: Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guangxi Medical University, Nanning 530021, China.
authors: Nuo
authors: N
authors: Zhou
authors: Xuan-ping
authors: XP
authors: Huang
authors: Ni
authors: N
authors: Liao
authors: Shan-liang
authors: SL
authors: Wei
authors: Fei-xin
authors: FX
authors: Liang
authors: Hua-ming
authors: HM
authors: Mai
mesh_terms: D055396:Bone Morphogenetic Protein 2; D019976:Cloning, Organism; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014162:Transfection
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2