Immunoassay using microfluid filters constructed by deep X-ray lithography.
Microfluid filters were fabricated, which possessed 2,100 cylindrical through-bores (psi 40 microm) in 200 microm-thickness polymethylmethacrylate (PMMA) sheets (psi 3 mm), by deep X-ray lithography using synchrotron radiation. To evaluate the microfluid filters as a device for an immunoassay, we bound the goat anti-mouse immunoglobulin G (IgG) antibody to the surface of the filters, and set the filters between reaction vessels stacked vertically in a microreactor. An enzyme-linked immunosorbent assay (ELISA) of mouse IgG using the goat anti-mouse IgG/horseradish-peroxidase (HRP) conjugate indicated that mouse IgG could be quantitatively detected in the range of 0-100 ng/ml, demonstrating the applicability of vertical microfluidic operation to the immunoassay.
Matsui
Katsuhiro
K
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, Himeji, Hyogo, Japan.
Kawaji
Isao
I
Utsumi
Yuichi
Y
Ukita
Yoshiaki
Y
Asano
Toshifumi
T
Takeo
Masahiro
M
Kato
Dai-ichiro
D
Negoro
Seiji
S
D000818:Animals; D006041:Goats; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D019904:Polymethyl Methacrylate; D014965:X-Rays
D007074:Immunoglobulin G; D019904:Polymethyl Methacrylate