title: A method for analyzing lipid-modified proteins with mass spectrometry.
abstract: In protein analysis using mass spectrometry, proteins are usually separated by electrophoresis and digested within the gel with proteases such as trypsin. However, analysis of lipid-modified proteins is difficult due to the low recovery of lipid-modified peptide fragments from the gel as well as their low ionization efficiency during mass spectrometry. In this study, we developed a simple extraction method with n-dodecyl-beta-D-maltoside following chloroform/methanol extraction that efficiently elutes lipid-modified fragments from gels. This method allowed us to analyze the structure of lipid-modified fragments, suggesting the applicability of the method for analysis of lipid-modified fragments by mass spectrometry.
authors: Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan.
authors: Tetsuro
authors: T
authors: Ujihara
authors: Isamu
authors: I
authors: Sakurai
authors: Naoki
authors: N
authors: Mizusawa
authors: Hajime
authors: H
authors: Wada
mesh_terms: D000595:Amino Acid Sequence; D002725:Chloroform / Q000737:chemistry; D004586:Electrophoresis; D005960:Glucosides / Q000737:chemistry; D008055:Lipids / Q000032:analysis* / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D000432:Methanol / Q000737:chemistry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000737:chemistry / Q000378:metabolism
chemical_list: D005960:Glucosides; D008055:Lipids; D010446:Peptide Fragments; D011506:Proteins; C040358:dodecyl maltoside; D002725:Chloroform; D014357:Trypsin; D000432:Methanol