[Evaluation of the Abbott Cell-Dyn Sapphire hematology analyzer].
BACKGROUND
The performance of Cell-Dyn Sapphire (Abbott Diagnostic, USA) was compared to the Bayer Advia 2120 (Bayer Diagnostics, USA), Sysmex XE-2100 (Sysmex Corporation, Japan), and reference microscopy.


METHODS
Three hundred samples for routine CBC and WBC differentials were randomly chosen for a comparison analysis. The Cell-Dyn Sapphire system was evaluated according to the linearity, imprecision, inter-instrument correlations, and white blood cell differential.


RESULTS
The CBC parameters (WBC, RBC, hemoglobin and platelet) showed a significant linearity with correlation coefficients greater than 0.99 (P<0.0001). Coefficients of variation (CV) for within-run and differential count of WBC were less than 5% except for Total CV for monocytes, eosinophils, and basophils and within-run CV for low valued eosinophils. The correlation coefficients with manual count were lower in monocytes, eosinophils, and basophils than in neutrophils and lymphocytes. The correlation with other hematology anlayzers was significant exclusive of basophils.


CONCLUSIONS
These results demonstrate that the Cell-Dyn Sapphire has a good linearity, an acceptable reproducibility, a minimal carryover, and a comparable performance with the sysmex XE-2100 and Advia 2120.
Park
Younhee
Y
Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea.
Song
Jaewoo
J
Song
Sungwook
S
Song
Kyung Soon
KS
Ahn
Mee Suk
MS
Yang
Mi-Sook
MS
Kim
Il
I
Choi
Jong Rak
JR
D000704:Analysis of Variance; D001322:Autoanalysis; D001772:Blood Cell Count / Q000295:instrumentation* / Q000379:methods; D001800:Blood Specimen Collection; D003951:Diagnostic Errors; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity