A super highly sensitive glucose biosensor based on Au nanoparticles-AgCl@polyaniline hybrid material.
Gold nanoparticles (AuNPs) with an average diameter of 5nm were assembled on the surface of silver chloride@polyaniline (PANI) core-shell nanocomposites (AgCl@PANI). Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) suggested that AuNPs were incorporated on AgCl@PANI through coordination bonds instead of electrostatic interaction. The resulting AuNPs-AgCl@PANI hybrid material exhibited good electroactivity at a neutral pH environment. An amperometric glucose biosensor was developed by adsorption of glucose oxidase (GOx) on an AuNPs-AgCl@PANI modified glassy carbon (GC) electrode. AuNPs-AgCl@PANI could provide a biocompatible surface for high enzyme loading. Due to size effect, the AuNPs in the hybrid material could act as a good catalyst for both oxidation and reduction of H(2)O(2). As the measurement of glucose was based on the electrochemical detection of H(2)O(2) generated by enzyme-catalyzed-oxidation of glucose, the biosensor exhibited a super highly sensitive response to the analyte with a detection limit of 4 pM. Moreover, the biosensor showed good reproducibility and operation stability. The effects of some factors, such as temperature and pH value, were also studied.
Yan
Wei
W
Key Laboratory of Analytical Chemistry for Life Science (MOE), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China.
Feng
Xiaomiao
X
Chen
Xiaojun
X
Hou
Wenhua
W
Zhu
Jun-Jie
JJ
D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018030:Silver Compounds / Q000737:chemistry*
D000814:Aniline Compounds; D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D018030:Silver Compounds; C416807:polyaniline; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose; C037548:silver chloride