Novel method for the synthesis of 2' -phosphorylated oligonucleotides.
We have developed a new method for the preparation of oligodeoxyribonucleotides and oligo(2'-O-methylribonucleotides) that contain a 2'-phosphorylated ribonucleoside residue, and optimized it to avoid 2' -3' -isomerization and chain cleavage. Structures of the 2' -phosphorylated oligonucleotides were confirmed by MALDI-TOF MS and enzymatic digestion, and the stability of their duplexes with DNA and RNA was investigated. 2'-Phosphorylated oligonucleotides may be useful intermediates for the introduction of various chemical groups for a wide range of applications.
Novopashina
Darya S
DS
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.
Totskaya
Olesya S
OS
Meschaninova
Maria I
MI
Stetsenko
Dmitry A
DA
Venyaminova
Alya G
AG
D002625:Chemistry, Organic / Q000379:methods*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010766:Phosphorylation
D009841:Oligonucleotides