A role for microRNAs in maintenance of mouse mammary epithelial progenitor cells.
microRNA (miRNA) expression profiles are often characteristic of specific cell types. The mouse mammary epithelial cell line, Comma-Dbeta, contains a population of self-renewing progenitor cells that can reconstitute the mammary gland. We purified this population and determined its miRNA signature. Several microRNAs, including miR-205 and miR-22, are highly expressed in mammary progenitor cells, while others, including let-7 and miR-93, are depleted. Let-7 sensors can be used to prospectively enrich self-renewing populations, and enforced let-7 expression induces loss of self-renewing cells from mixed cultures.
Ibarra
Ingrid
I
Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Erlich
Yaniv
Y
Muthuswamy
Senthil K
SK
Sachidanandam
Ravi
R
Hannon
Gregory J
GJ
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D004847:Epithelial Cells / Q000166:cytology; D008321:Mammary Glands, Animal / Q000166:cytology*; D051379:Mice; D035683:MicroRNAs / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology*
D017931:DNA Primers; D035683:MicroRNAs