Globin gene enhancer activity of a DNase-I hypersensitive site-40 homolog in medaka, Oryzias latipes.
In medaka, we found a C16orf35-like gene in the region within 1 Kbp 3' downstream of the psibeta end of the 36-Kbp embryonic globin gene cluster ((5')alpha0(3')-(3')beta1(5')-(5')alpha1(3')-(5')beta2(3')-(5')alpha2(3')-(3')alpha3(5')-(5')beta3(3')-(3')beta4(5')-(5')alpha4(3')-(3')psialpha(5')-(5')psibeta(3')). Intron 5 of the gene contained a region having NF-E2 binding sites located between GATA boxes. The region was homologous to human HS-40 in terms of the existence and structure of characteristic transcription-factor binding sites and was named Ol-HS-40. Injection of the fusion gene construct Ol-HS-40-alpha0(up-2)GFP, consisting of Ol-HS-40, a 5' upstream 200-bp minimum promoter for alpha0, and green fluorescent protein (GFP), showed that Ol-HS-40, as in human HS-40, had the ability to strongly enhance GFP expression in erythroid cells of embryos. Further analysis using transgenic technology revealed that Ol-HS-40 had the ability to change the type of the GFP expression from embryo-to-young fish to embryo-to-adult. In addition, the results suggest that Ol-HS-40, although its natural function remains unclear, has strong enhancer activity for the expression of not only the alpha-globin gene but also the beta-globin gene.
Maruyama
Kouichi
K
National Institute of Radiological Science, 4-9-1 Anagawa, Inage-ku, Chiba, Japan. k_maruya@nirs.go.jp
Ishikawa
Yuji
Y
Yasumasu
Shigeki
S
Iuchi
Ichiro
I
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D003850:Deoxyribonuclease I / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000502:physiology; D004742:Enhancer Elements, Genetic / Q000502:physiology*; D041905:Erythroid Cells; D005786:Gene Expression Regulation; D023061:Gene Order; D005914:Globins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000032:analysis; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009990:Oryzias / Q000235:genetics*
D049452:Green Fluorescent Proteins; D005914:Globins; D003850:Deoxyribonuclease I