Determination of sulpiride in human urine using excitation-emission matrix fluorescence coupled with second-order calibration.
This paper proposes a new and effective approach for the quantitative analysis of sulpiride, a significant antipsychotic drug, in human urine samples by the incorporation of excitation-emission matrix (EEM) fluorescence and second-order calibration methodologies based on the alternating fitting residue (AFR) and self-weighted alternating trilinear decomposition (SWATLD) algorithms. With the application of a second-order advantage, the proposed strategy could be utilized for a direct concentration determination of sulpiride with a simple pretreatment step, even in the presence of serious natural fluorescent interferences. The average recoveries of sulpiride in complex urine samples by using AFR and SWATLD with an estimated component number of three were 101.2 +/- 2.1 and 94.4 +/- 0.7%, respectively. Moreover, the accuracy of the two algorithms was also evaluated through elliptical joint confidence region (EJCR) tests as well as the figures of merit, such as sensitivity (SEN), selectivity (SEL) and limit of detection (LOD). The experimental results demonstrated that both algorithms, as promising quantitative alternatives, have been satisfactorily applied to the determination of sulpiride in human urine, but the performance of AFR was slightly better than that of SWATLD.
Nie
Jin-Fang
JF
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.
Wu
Hai-Long
HL
Xia
A-Lin
AL
Zhu
Shao-Hua
SH
Bian
Ying-Chao
YC
Li
Shu-Fang
SF
Yu
Ru-Qin
RQ
D000465:Algorithms; D014150:Antipsychotic Agents / Q000652:urine*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013050:Spectrometry, Fluorescence; D013469:Sulpiride / Q000652:urine*
D014150:Antipsychotic Agents; D013469:Sulpiride