Study of UVA irradiation on hemoglobin in the presence of NADH.
Reduction of ferric methemoglobin (metHb) to its ferrous form is observed by short-time ultraviolet A (UVA) irradiation of metHb together with nicotinamide adenine dinucleotide (NADH). And, severely structural destruction of metHb occurs when long-time UVA irradiation is exerted. However, neither reduction nor destruction can be observed in the absence of NADH under otherwise the same experimental conditions. Accordingly, the O2-binding ability of the protein increases by short-time UVA irradiation of metHb together with NADH, which corresponds with the reduction of metHb, while it decreases by long-time UVA irradiation, which corresponds with the structural destruction. Besides, it is found that the reduction reaction and the conformational destruction proceed more rapidly with higher concentrations of NADH.
Liang
Xiaoqiang
X
School of Life Science and Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University, Shanghai 200444, PR China.
Chen
Guifang
G
Zhang
Xin
X
Liu
Shanli
S
Li
Genxi
G
D006454:Hemoglobins / Q000737:chemistry* / Q000528:radiation effects*; D006801:Humans; D009243:NAD / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry; D013053:Spectrophotometry; D014466:Ultraviolet Rays
D006454:Hemoglobins; D009243:NAD; D010100:Oxygen