title: The DEAD-box protein Dbp5 controls mRNA export by triggering specific RNA:protein remodeling events.
abstract: Messenger RNA (mRNA) export involves the unidirectional passage of ribonucleoprotein particles (RNPs) through nuclear pore complexes (NPCs), presumably driven by the ATP-dependent activity of the DEAD-box protein Dbp5. Here we report that Dbp5 functions as an RNP remodeling protein to displace the RNA-binding protein Nab2 from RNA. Strikingly, the ADP-bound form of Dbp5 and not ATP hydrolysis is required for RNP remodeling. In vivo studies with nab2 and dbp5 mutants show that a Nab2-bound mRNP is a physiological Dbp5 target. We propose that Dbp5 functions as a nucleotide-dependent switch to control mRNA export efficiency and release the mRNP from the NPC.
authors: Department of Cell and Developmental Biology, Vanderbilt University Medical Center, U-3209 MRBIII, 465 21st Avenue South, Nashville, TN 37232-8240, USA.
authors: Elizabeth J
authors: EJ
authors: Tran
authors: Yingna
authors: Y
authors: Zhou
authors: Anita H
authors: AH
authors: Corbett
authors: Susan R
authors: SR
authors: Wente
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000502:physiology; D001665:Binding Sites; D003432:Cross-Linking Reagents; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D017403:In Situ Hybridization; D022022:Nuclear Pore / Q000378:metabolism / Q000648:ultrastructure; D029543:Nucleocytoplasmic Transport Proteins / Q000235:genetics / Q000378:metabolism*; D010833:Phytic Acid / Q000378:metabolism*; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D003432:Cross-Linking Reagents; C080773:NAB2 protein, S cerevisiae; D029543:Nucleocytoplasmic Transport Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D029701:Saccharomyces cerevisiae Proteins; C026452:messenger ribonucleoprotein; D010833:Phytic Acid; C492382:DBP5 protein, S cerevisiae; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases