title: Rescue of maturation-defective flock house virus infectivity with noninfectious, mature, viruslike particles.
abstract: The infectivity of flock house virus (FHV) requires autocatalytic maturation cleavage of the capsid protein at residue 363, liberating the C-terminal 44-residue gamma peptides, which remain associated with the particle. In vitro studies previously demonstrated that the amphipathic, helical portion (amino acids 364 to 385) of gamma is membrane active, suggesting a role for gamma in RNA membrane translocation during infection. Here we show that the infectivity of a maturation-defective mutant of FHV can be restored by viruslike particles that lack the genome but undergo maturation cleavage. We propose that the colocalization of the two defective particle types in an entry compartment allows the restoration of infectivity by gamma.
authors: Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd. Mail MB-31, La Jolla, CA 92037, USA. jackj@scripps.edu
authors: Hanna E
authors: HE
authors: Walukiewicz
authors: Manidipa
authors: M
authors: Banerjee
authors: Anette
authors: A
authors: Schneemann
authors: John E
authors: JE
authors: Johnson
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D004330:Drosophila / Q000821:virology; D030381:Nodaviridae / Q000235:genetics / Q000502:physiology*; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D014771:Virion / Q000502:physiology*; D053586:Virus Internalization
chemical_list: D036022:Capsid Proteins; D010446:Peptide Fragments