Human articular chondrocytes culture.
In this chapter, we describe a strategy to expand adult human articular chondrocytes in monolayer while maintaining high post-expansion chondrogenic capacity. This method relies on the use of a specific growth factor cocktail during the two-dimensional (2D) propagation of chondrocytes. In addition, a protocol to isolate and grow single-colony-derived strains of chondrocytes is presented as a model to study the biology of different chondrocyte subpopulations.
Barbero
Andrea
A
Institute for Surgical Research and Hospital Management, University Hospital Basel, Switzerland.
Martin
Ivan
I
D000328:Adult; D002358:Cartilage, Articular / Q000166:cytology*; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology; D006801:Humans