Global transcriptional responses of Pseudomonas aeruginosa to phage PRR1 infection.
The infectious cycles of viruses are known to cause dramatic changes to host cell function. The development of microarray technology has provided means to monitor host cell responses to viral infection at the level of global changes in mRNA levels. We have applied this methodology to investigate gene expression changes caused by a small, icosahedral, single-stranded-RNA phage, PRR1 (a member of the Leviviridae family), on its host, Pseudomonas aeruginosa, at different times during its growth cycle. Viral infection in this system resulted in changes in expression levels of <4% of P. aeruginosa genes. Interestingly, the number of genes affected by viral infection was significantly lower than the number of genes affected by changes in growth conditions during the experiment. Compared with a similar study that focused on the complex, double-stranded-DNA bacterial virus PRD1, it was evident that there were no universal responses to viral infection. However, in both cases, translation was affected in infected cells.
Ravantti
Janne J
JJ
Viikki Biocenter 2, P.O. Box 56, Viikinkaari 5, FIN-00014 University of Helsinki, Finland.
Ruokoranta
Tanja M
TM
Alapuranen
A Marika
AM
Bamford
Dennis H
DH
D001435:Bacteriophages / Q000235:genetics / Q000472:pathogenicity*; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D018076:DNA, Complementary; D005814:Genes, Viral; D020411:Oligonucleotide Array Sequence Analysis; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000821:virology; D012329:RNA, Bacterial / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D015854:Up-Regulation
D017931:DNA Primers; D004269:DNA, Bacterial; D018076:DNA, Complementary; D012329:RNA, Bacterial