First molecular detection of Borrelia afzelii in clinical samples in Korea.
Borrelia afzelii nucleic acids were detected in the sera of febrile disease patients by a nested PCR that targeted the rrf (5S)-rrl (23S) spacer of B. burgdorferi sensu lato. The B. afzelii-specific DNA fragment was detected in 8 out of 283 sera which were proven to have immunoglobulin G or M antibodies against B. burgdorferi antigens through IFA. The results were further confirmed through restriction fragment length polymorphism and sequencing analysis of the DNA fragments. The results indicated for the first time that Lyme borreliosis is prevalent in Korea.
Choi
Yeon-Joo
YJ
Department of Microbiology, College of Medicine, Konkuk University, Seoul, Republic of Korea.
Han
Seung-Hoon
SH
Park
Jin-Mi
JM
Lee
Kyung-Min
KM
Lee
Eun-Mi
EM
Lee
Seung-Hyun
SH
Song
Hyeon-Je
HJ
Koh
Young-Sang
YS
Lee
Keun-Wha
KW
Jang
Won-Jong
WJ
Park
Kyung-Hee
KH
D000907:Antibodies, Bacterial / Q000097:blood; D000954:Antigens, Surface / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001428:Bacterial Vaccines / Q000235:genetics; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000097:blood / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000097:blood / Q000737:chemistry / Q000235:genetics; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007723:Korea; D008074:Lipoproteins / Q000235:genetics; D008193:Lyme Disease / Q000175:diagnosis* / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D044967:Serum / Q000382:microbiology
D000907:Antibodies, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008074:Lipoproteins; C072045:OspA protein