Complete DNA sequence and analysis of the transferable multiple-drug resistance plasmids (R Plasmids) from Photobacterium damselae subsp. piscicida isolates collected in Japan and the United States.
Photobacterium damselae subsp. piscicida is a bacterial fish pathogen that causes a disease known as pasteurellosis. Two transferable multiple-drug resistance (R) plasmids, pP99-018 (carrying resistance to kanamycin, chloramphenicol, tetracycline, and sulfonamide) and pP91278 (carrying resistance to tetracycline, trimethoprim, and sulfonamide), isolated from P. damselae subsp. piscicida strains from Japan (P99-018) and the United States (P91278), respectively, were completely sequenced and analyzed, along with the multiple-drug resistance regions of three other R plasmids also from P. damselae subsp. piscicida strains from Japan. The sequence structures of pP99-018 (150,057 bp) and pP91278 (131,520 bp) were highly conserved, with differences due to variation in the drug resistance and conjugative transfer regions. These plasmids, shown to be closely related to the IncJ element R391 (a conjugative, self-transmitting, integrating element, or constin), were divided into the conjugative transfer, replication, partition, and multiple-drug resistance regions. Each of the five multiple-drug resistance regions sequenced exhibited unique drug resistance marker composition and arrangement.
Kim
Mi-Jung
MJ
Laboratory of Genome Science, Graduate School of Marine Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
Hirono
Ikuo
I
Kurokawa
Ken
K
Maki
Takeshi
T
Hawke
John
J
Kondo
Hidehiro
H
Santos
Mudjekeewis D
MD
Aoki
Takashi
T
D000818:Animals; D001492:Bass / Q000382:microbiology; D003227:Conjugation, Genetic; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005393:Fish Diseases / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D007564:Japan; D008969:Molecular Sequence Data; D010473:Perciformes / Q000382:microbiology; D010776:Photobacterium / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D011815:R Factors / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014481:United States