title: Subcutaneous transplantation of autologous oral mucosal epithelial cell sheets fabricated on temperature-responsive culture dishes.
abstract: The oral mucosa is an attractive cell source for autologous transplantation in human patients who require regenerative therapies of various epithelia. However, the time-course of cellular changes in transplanted oral mucosal epithelia at ectopic sites remains poorly understood. By applying a rat model, we analyzed phenotypic changes in oral mucosal epithelial cell sheets after harvest from temperature-responsive culture dishes and subsequent autologous subcutaneous transplantation. We used monoclonal antibodies to identify epithelial-specific cytokeratins 4, 10, 13, and 14, the stem/progenitor cell marker p63, and proliferating cell nuclear antigen, within the regenerated tissues. Transplanted oral mucosal epithelial cell sheets proliferated during the first week after grafting in conjunction with host inflammation, but then began to degenerate afterward with complete disappearance after 3 weeks. Our findings suggest that host subcutaneous tissues support proliferation and differentiation of the oral mucosal epithelial cell sheets, but are unable to promote maintenance of stem and progenitor cells and therefore cannot produce long-term survivability.
authors: Graduate School of Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan.
authors: Haruko
authors: H
authors: Obokata
authors: Masayuki
authors: M
authors: Yamato
authors: Joseph
authors: J
authors: Yang
authors: Kohji
authors: K
authors: Nishida
authors: Satoshi
authors: S
authors: Tsuneda
authors: Teruo
authors: T
authors: Okano
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation*; D017690:Cell Transplantation; D004847:Epithelial Cells / Q000166:cytology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D008297:Male; D009061:Mouth Mucosa / Q000166:cytology* / Q000648:ultrastructure; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D013234:Stem Cells / Q000166:cytology; D040521:Subcutaneous Tissue / Q000637:transplantation*; D013696:Temperature; D023822:Tissue Engineering; D014182:Transplantation, Autologous
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D007633:Keratins