[The protective effect of molsidomin in immune ovarian pathology in mice].
Experimental immune ovarian failure in CBA mice was induced by either administration of xenogenic anti-ovarian antibodies (scheme 1) or immunization with allogenic ovarian extracts (scheme 2). It was shown that both types of treatment impaired the meiotic maturation of oocytes: the number of cells at the stages of metaphase I and metaphase II decreased compared to the cells of control mice. In both schemes of experiments, impaired oogenesis was accompanied by reduction of percentage of viable follicular cells and by increase in the part of cells possessing morphological features of apoptosis. In contrast, the number of necrotic follicular cells increased in scheme 2 only. The donor of nitric oxide molsidomin (10 mg/ kg), when injected an hour before administration of xenogenic anti-ovarian antibodies or allogenic ovary extracts, improved the meiotic maturation of oocytes and favored follicular, lymph nodes and thymus cells survival by decreasing the number of apoptotic and necrotic cells.
Makohon
N V
NV
Voznesens'ka
T Iu
TIu
Bryzhina
T M
TM
Sukhina
V S
VS
Hrushka
N H
NH
Aleksieieva
I M
IM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology*; D016923:Cell Death / Q000187:drug effects / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005260:Female; D008540:Meiosis / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D008981:Molsidomine / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009865:Oocytes / Q000187:drug effects / Q000276:immunology; D009866:Oogenesis / Q000187:drug effects / Q000276:immunology*; D010053:Ovary / Q000187:drug effects / Q000276:immunology*
D000911:Antibodies, Monoclonal; D020030:Nitric Oxide Donors; D008981:Molsidomine