title: cDNA microarray analysis of the differentially expressed genes involved in murine pre-osteoclast RAW264.7 cells proliferation stimulated by dexamethasone.
abstract: Glucocorticoids (GCs) are hormones with anti-inflammatory and immuno-suppressive effects. The use of hormonal medicine like GCs may cause systemic adverse effects. In the present study, the cellular response of murine pre-osteoclast cell line RAW264.7 to dexamethasone (DEX) was investigated and the result demonstrated that DEX may stimulate RAW264.7 cells proliferation. Changes in gene expression involved in RAW264.7 cells proliferation stimulated by dexamethasone were investigated using cDNA microarrays containing 1000 cDNAs. It was found that 67 genes were regulated by DEX and could be grouped into 8 functional categories, including cell cycle regulation, cell survival, metabolism, pro-inflammatory effect, cytoskeleton, proteasome, signaling transduction and transcription factors. Moreover, some signaling pathways that involve in modulation of DEX on RAW264.7 cells functions were identified, including p53, 14-3-3 gamma, MAPK, Elk-1, I kappa B and Ifn related pathways.
authors: Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong, China.
authors: Qi
authors: Q
authors: Zhang
authors: Chi-Chun
authors: CC
authors: Fong
authors: Yaou
authors: Y
authors: Zhang
authors: Chi-Hung
authors: CH
authors: Tzang
authors: Wang-Fun
authors: WF
authors: Fong
authors: Mengsu
authors: M
authors: Yang
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D005938:Glucocorticoids / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D010010:Osteoclasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051381:Rats
chemical_list: D005938:Glucocorticoids; D003907:Dexamethasone