Toll-like receptor 4 and cytokine expression involved in functional immune response in an originally established porcine intestinal epitheliocyte cell line.
To study the immune responses of porcine intestinal epithelial cells to gram-negative bacteria via toll-like receptors (TLRs), originally established porcine intestinal epitheliocyte (PIE) cells were treated with lipopolysaccharide (LPS) or swine-specific enterotoxigenic Escherichia coli (ETEC). Real-time quantitative PCR revealed that PIE cells expressed TLR1-9 and MD-2 mRNAs, preferentially expressed TLR4/MD-2. Immunostaining of PIE cells revealed that TLR4 was precisely expressed in PIE cells at the protein level. PIE cells treated with LPS had up-regulated expression of several TLRs (TLR2, 3, 4, 5 and 8), type 1 helper T (Th1) cytokines (interleukin (IL)-1alpha, IL-1beta, IL-6, IL-15, 18, leukemia inhibitory factor (LIF), and interferon (IFN)-beta), and chemokines (monocyte chemoattractant protein (MCP)-1 and IL-8). ETEC enhanced the expression of TLR2, Th1 type cytokines (IL-1alpha, IL-12p35 and IL-6) and chemokines (MCP-1 and IL-8). These results indicate that PIE induces inflammatory responses by up-regulating Th1 cytokines and chemokines in response to LPS or ETEC, suggesting that PIE is a useful cell line for studying inflammatory responses via TLR4/MD-2 in intestinal epithelial cells.
Moue
Masayuki
M
Food Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Aobaku, Sendai 981-8555, Japan.
Tohno
Masanori
M
Shimazu
Tomoyuki
T
Kido
Taketomo
T
Aso
Hisashi
H
Saito
Tadao
T
Kitazawa
Haruki
H
D000818:Animals; D002460:Cell Line / Q000276:immunology*; D016207:Cytokines / Q000032:analysis / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D013552:Swine; D051197:Toll-Like Receptor 4 / Q000032:analysis / Q000378:metabolism*
D016207:Cytokines; D008070:Lipopolysaccharides; D051197:Toll-Like Receptor 4