Inhibition of Listeria monocytogenes in dairy products using the bacteriocin-like peptide cerein 8A.
The efficacy of the antimicrobial peptide cerein 8A to control the development of Listeria monocytogenes in milk and soft cheese was investigated. The addition of 160 AU ml(-1) cerein 8A to UHT milk resulted in a decrease of 3 log cycles in viable cells within the 14-day period at 4 degrees C. The viable counts of L. monocytogenes in pasteurized milk samples containing cerein 8A was lower than those observed in controls without bacteriocin. Addition of cerein 8A to Minas-type soft cheese caused a delay in the start of exponential growth phase, although similar counts were observed after day 6. When cerein 8A was used to control cheese surface contamination by L. monocytogenes, a decrease of 2 log cycles in viable counts of cerein-treated samples was observed during 30 days at 4 degrees C. This antimicrobial peptide shows potential use as a biopreservative for application in dairy products.
Bizani
Delmar
D
Laboratório de Bioquímica e Microbiologia Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 91501-970 Porto Alegre, Brazil.
Morrissy
Juliana A C
JA
Dominguez
Ana P M
AP
Brandelli
Adriano
A
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001409:Bacillus cereus / Q000378:metabolism*; D001430:Bacteriocins / Q000494:pharmacology*; D002611:Cheese / Q000382:microbiology; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D003611:Dairy Products / Q000382:microbiology*; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control; D005519:Food Preservation / Q000379:methods; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000382:microbiology
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C505873:cerein 8A, Bacillus cereus