[The expression of estrogen receptors in the human periodontal ligament fibroblasts in vitro].
OBJECTIVE
To explore the expression of estrogen receptors in the human periodontal ligament fibroblasts in vitro.


METHODS
Human periodontal ligament fibroblasts were cultured in vitro. The estrogen receptors (ER)-alpha, ER-beta were detected with immunocytochemistry staining; The mRNA of ER-alpha, ER-beta were measured by reverse transcription polymerase chain reaction; The expression of ER-alpha, ER-beta protein were detected by Western blot.


RESULTS
The mRNA and protein expression of two subtype of ER were observed on human periodontal ligament fibroblasts (HPLF). The intensity of the ER-beta bands were stronger than those of ER-alpha, indicating that ER-beta expression in HPLF was higher.


CONCLUSIONS
ER may play an important role in the function of human periodontal ligament fibroblasts.
Cao
Meng
M
Department of Orthodontics, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Ding
Yin
Y
Shu
Lei
L
Li
Jia
J
Zhang
Wei
W
Guo
Tao
T
D000293:Adolescent; D015153:Blotting, Western; D002478:Cells, Cultured; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D010513:Periodontal Ligament / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012333:RNA, Messenger