title: Production of transgenic goat (Capra hircus) with human Granulocyte Colony Stimulating Factor (hG-CSF) gene in Brazil.
abstract: In order to produce transgenic goats with hG-CSF, a total of 24 adult Saanen and 48 adult undefined breed goats were used as donors and recipients, respectively. Donors were estrus-synchronized with vaginal sponges and superovulated by a treatment with 200 mg FSH given twice daily in decreasing doses over 3 days starting 48 h before sponge removal. Ovulation was induced by injecting 100 microg GnRH 36 h after sponge removal. The recipients also received an estrus synchronization treatment. Donors were mated with fertile Saanen bucks and, approximately 72 h after sponge removal, zygotes were recovered surgically by flushing oviducts. The recovered zygotes were briefly centrifuged to a reliable visualization of the pronuclei. The DNA construct containing hG-CSF gene flanked by goat and bovine alphas1-casein sequences was injected into pronuclei of 129 zygotes. The microinjected embryos (3-6 per female) were transferred to 27 recipients. Ten recipients became pregnant and 12 kids were born. One transgenic male founder was identified in the group of kids. This is the first report of a birth of a transgenic goat in Latin America.
authors: Laboratório de Fisiologia e Controle da Reprodução, Universidade Estadual do Ceará, Fortaleza, CE, Brazil. vjff@pq.cnpq.br
authors: Vicente J F
authors: VJ
authors: Freitas
authors: Irina A
authors: IA
authors: Serova
authors: Lyudmila E
authors: LE
authors: Andreeva
authors: Guennadi A
authors: GA
authors: Dvoryanchikov
authors: Edilson S
authors: ES
authors: Lopes
authors: Dárcio I A
authors: DI
authors: Teixeira
authors: Luciene P B
authors: LP
authors: Dias
authors: Suely R G
authors: SR
authors: Avelar
authors: Raylene R
authors: RR
authors: Moura
authors: Luciana M
authors: LM
authors: Melo
authors: Alexsandra F
authors: AF
authors: Pereira
authors: João B
authors: JB
authors: Cajazeiras
authors: Maria L L
authors: ML
authors: Andrade
authors: Karlliely C
authors: KC
authors: Almeida
authors: Karlliely A
authors: KA
authors: Castro
authors: Francisco C
authors: FC
authors: Sousa
authors: Antonio C C
authors: AC
authors: Carvalho
authors: Oleg L
authors: OL
authors: Serov
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000196:embryology*; D001938:Brazil; D004624:Embryo Transfer; D005260:Female; D006041:Goats / Q000196:embryology / Q000235:genetics*; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics*; D008297:Male; D008845:Microinjections; D011247:Pregnancy; D015053:Zygote / Q000502:physiology
chemical_list: D016179:Granulocyte Colony-Stimulating Factor