Serum PON1 arylesterase activity in relation to hyperhomocysteinaemia and oxidative stress in young adult central retinal venous occlusion patients.
OBJECTIVE
To estimate the arylesterase activity of serum paraoxonase-1 (PON1-ARE), which is reported to have an antioxidant and antiatherogenic potential and to correlate with plasma homocysteine (Hcys) and plasma TBARS in young adult central retinal venous occlusion (CRVO) patients.


METHODS
A case-control prospective study carried out in 10 CRVO patients (mean age 27+/-5 years; 7 males, 3 females) and 20 healthy controls (mean age 29+/-5 years; 15 males, 5 females).


RESULTS
The CRVO patients showed a significantly lowered serum PON1-ARE activity (P=0.009) along with a significant increase in the levels of plasma Hcys (P=0.018) when compared to the control subjects. There was a negative correlation between serum PON1-ARE and plasma Hcys levels (P=0.058) as well as between PON1-ARE and plasma TBARS levels (P=0.001) in the CRVO patients.


CONCLUSIONS
This is the first report of lowered serum PON1-ARE level as a risk factor for CRVO (OR= 1.108, CI=0.914, 1.314; P=0.296), which is found to correlate with oxidative stress.
Angayarkanni
N
N
Biochemistry Research Department, Sankara Nethralaya, Vision Research Foundation, Chennai, India. drak@snmail.org
Barathi
S
S
Seethalakshmi
T
T
Punitham
R
R
Sivaramakrishna
R
R
Suganeswari
G
G
Tarun
S
S
D000328:Adult; D043303:Aryldialkylphosphatase / Q000097:blood*; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D020138:Hyperhomocysteinemia / Q000150:complications* / Q000201:enzymology; D008297:Male; D018384:Oxidative Stress; D012170:Retinal Vein Occlusion / Q000097:blood / Q000201:enzymology* / Q000209:etiology*; D012307:Risk Factors; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D055815:Young Adult
D017392:Thiobarbituric Acid Reactive Substances; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human