[Mental hospital outbreak of Mycobacterium tuberculosis with few copies of IS6110 DNA fingerprinting].
We report a case of 4 patients with pulmonary tuberculosis in a mental hospital located in Yamanashi, Japan, between 2000 and 2004 in which 3 isolates of Mycobacterium tuberculosis were analyzed using IS6110 restriction fragment length polymorphism (RFLP). DNA fingerprinting showed them to be identical and the copy number to be two. Additional RFLP using (CGG)5 fingerprinting yielded the same result and a copy number of 15, suggesting a M. tuberculosis outbreak. (CGG)5 analysis thus proved useful in supplementary typing method like polymorphic G-C rich repetitive sequence (PGRS) or spoligotyping.
Takahara
Makoto
M
Department of Internal Medicine, National Hospital Organization Nishi-Kofu Hospital.
Kirikae
Teruo
T
D000368:Aged; D016172:DNA Fingerprinting / Q000379:methods*; D004196:Disease Outbreaks; D005260:Female; D005798:Genes, Bacterial; D006778:Hospitals, Psychiatric; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology* / Q000382:microbiology