Evaluation of the NucliSens miniMAG RNA extraction and real-time NASBA applications for the detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in throat swabs.
Nucleic acids were extracted from 215 throat swabs from patients with community-acquired pneumonia by the manual Boom extraction, the NucliSens miniMAG and the Qiagen DNA blood kit and amplified respectively by in-house real-time NASBA, NucliSens EasyQ reagents, and real-time PCR for the detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae. Five out of 215 throat swabs were found to be C. pneumoniae positive by all techniques used. A total of 11 out of 215 throat swabs were positive for M. pneumoniae; 10/215 by Qiagen extraction and PCR amplification and 9/215 by NucliSens miniMAG and NucliSens EasyQ amplification. The NucliSens miniMAG and NucliSens EasyQ applications were successfully coupled to detect both organisms in throat swabs.
Loens
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K
Department of Microbiology, University of Antwerp UA, Universiteitsplein 1 S009a, Antwerp-Wilrijk, Belgium. Katherine.loens@ua.ac.be
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Goossens
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Ieven
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D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D009177:Mycoplasma pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D010614:Pharynx / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D021161:Self-Sustained Sequence Replication / Q000379:methods*
D012329:RNA, Bacterial; D011933:Reagent Kits, Diagnostic