Effect of dietary epigallocatechin-3-gallate on cytochrome P450 2E1-dependent alcoholic liver damage: enhancement of fatty acid oxidation.
This study was designed to determine whether dietary epigallocatechin-3-gallate (EGCG), the most abundant catechin polyphenol in green tea, can protect the liver from cytochrome P450 2E1 (CYP2E1)-dependent alcoholic liver damage. Compared with an ethanol group, when EGCG was present in the ethanol diet, the formation of a fatty liver was significantly reduced and the serum aspartate transaminase (AST) and alanine transaminase (ALT) levels were much lower. Ethanol treatment significantly elevated hepatic CYP2E1 expression while simultaneously reducing hepatic phospho-acetyl CoA carboxylase (p-ACC) and carnitine palmitoyl-transferase 1 (CPT-1) levels. While EGCG markedly reversed the effect of ethanol on hepatic p-ACC and CPT-1 levels, it had no effect on the ethanol-induced elevation in CYP2E1 expression. EGCG prevents ethanol-induced hepatotoxicity and inhibits the development of a fatty liver. These effects were associated with improvements in p-ACC and CPT-1 levels. The use of EGCG might be useful in treating patients with an alcoholic fatty liver.
Yun
Jun-Won
JW
Amorepacific Corporation R&D Center, Yongin-si, Gyeonggi-do, Republic of Korea.
Kim
Young-Kyung
YK
Lee
Byoung-Seok
BS
Kim
Chae-Wook
CW
Hyun
Jin-Sook
JS
Baik
Joo-Hyun
JH
Kim
Jung-Ju
JJ
Kim
Bae-Hwan
BH
D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D004789:Enzyme Activation; D005235:Fatty Liver, Alcoholic / Q000188:drug therapy* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D002392:Catechin; C045651:epigallocatechin gallate; D019392:Cytochrome P-450 CYP2E1; D002334:Carnitine O-Palmitoyltransferase; D001219:Aspartate Aminotransferases; D000103:Acetyl-CoA Carboxylase