[Separation, purification and spectrum analysis of SHP].
Crude SHP was obtained from extracting with hot water and precipitating with ethyl alcohol. Free proteins were removed by trypsinase combined with Sevage method. Different fractions were got by gel filtration chromatography. The molecule structure was analyzed by ultraviolet spectrum scanning and KBr infrared spectroscope. The results showed that the reaction of phenol-sulfuric acid was postiive, reaction of ninhydrin was weak positive and reaction of iodine-potassium iodide was negative, the extraction was regarded as a non-starch polysaccharide. Extraction rate of SHP was 8.56% and the content of polysaccharides was 90.96%. Gel filtration chromatography showed that SHP composed of furanopolysaccharides, xylose, galactose, arabinose, glucose, rhamnose and fructose. Ultraviolet spectrum showed that SHP contained little DNA and protein, infrared spectrum showed that SHP was main furanopolysaccharides and had beta-glycosidic bouds in its molecule structure, and there was a characteristic absorb peak of at-D-galactopyranosyl in it.
Song
Yun-duan
YD
Analysis Center of Guangdong Medical College, Zhanjiang 524023, China. xiangxiang53@sohu.com
Meng
Qing-yong
QY
Xu
Mei-yi
MY
D001089:Arabinose / Q000032:analysis / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D044647:Sargassum / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D014990:Xylans / Q000032:analysis / Q000737:chemistry
D011134:Polysaccharides; D014990:Xylans; D001089:Arabinose