[Generation of cell culture high-yield recombinant H3N2 subtype swine influenza vaccine candidate by reverse genetics].
High-yield H3N2 subtype swine influenza virus for large-scale vaccine production in cell culture was generated by reverse genetics. The rescued H3N2 (rH3N2) candidate virus contained hemagglutinin (HA) and neuraminidase (NA) genes derived from a field isolate A/Swine/Henan/S4/01 (H3N2), PB2 gene from A/PR/8/34, and the other five internal genes from A/Goose/Dalian/3/01 (H9N2). The rH3N2 virus titer in MDCK cell culture were measured by hemagglutination assay and the maximum virus titre of 1:512 hemagglutination unit was obtained after infection of MDCK cell for 60 h. The results of the present study indicated that rH3N2 virus was suitable for growth in MDCK cell culture and is feasible to be used for the production of cell grown influenza vaccine.
Yang
Tao
T
National Key Laboratory of Veterinary Biotechnology and Animal Influenza Laboratory of the Ministry of Agriculture, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Liu
Ming
M
Liu
Chun-Guo
CG
Zhang
Yun
Y
Liu
Da-Fei
DF
Chen
Hao
H
Tong
Guang-Zhi
GZ
D000818:Animals; D002460:Cell Line; D004285:Dogs; D006386:Hemagglutination Tests; D053122:Influenza A Virus, H3N2 Subtype / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D007252:Influenza Vaccines; D010957:Plasmids; D014776:Virus Cultivation
D007252:Influenza Vaccines