title: Host glycoprotein Gp96 and scavenger receptor SREC interact with PorB of disseminating Neisseria gonorrhoeae in an epithelial invasion pathway.
abstract: Neisseria gonorrhoeae expresses numerous surface proteins that mediate bacterial adherence and invasion during infection. Gonococci expressing serotype A of the major outer membrane porin PorB (PorB(IA)) are frequently isolated from patients with severe disseminating infections. PorB(IA) triggers efficient adherence and invasion under low phosphate conditions mimicking systemic bloodstream infections. Here, we identify the human heat shock glycoprotein Gp96 and the scavenger receptor SREC as PorB(IA)-specific receptors. Gonococci expressing PorB(IA), but not those expressing PorB serotype B instead, bind to purified native or recombinant Gp96. Depletion of Gp96 from host cells prevented adherence but significantly triggered gonococcal invasion. Furthermore, such invasion was blocked by chemical inhibitors of scavenger receptors, and we identified SREC as the scavenger receptor involved in PorB(IA)-dependent invasion. Thus, we establish Gp96 as an anti-invasion factor and SRECs as receptors mediating host cell entry of highly invasive disseminating gonococci.
authors: Max Planck Institute for Infection Biology, Department of Molecular Biology, Research Group for Molecular Infection and Cancer Biology, Charitéplatz 1, Berlin D-10117, Germany.
authors: Cindy
authors: C
authors: Rechner
authors: Christiane
authors: C
authors: Kühlewein
authors: Anne
authors: A
authors: Müller
authors: Hansjörg
authors: H
authors: Schild
authors: Thomas
authors: T
authors: Rudel
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology; D006069:Gonorrhea / Q000382:microbiology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000502:physiology*; D009344:Neisseria gonorrhoeae / Q000472:pathogenicity / Q000502:physiology*; D018272:Porins / Q000378:metabolism*; D011485:Protein Binding; D051128:Scavenger Receptors, Class F / Q000502:physiology*
chemical_list: D008562:Membrane Glycoproteins; D018272:Porins; C495126:SCARF1 protein, human; D051128:Scavenger Receptors, Class F; C053387:endoplasmin; C057699:porin protein, Neisseria