Obtainment of polyclonal antibodies to clenbuterol with the use of colloidal gold.
The effectiveness of an in vivo synthesis of antibodies to clenbuterol with the use of gold nanoparticles as a carrier was evaluated. For comparison, conjugates of clenbuterol with bovine serum albumin were used in immunization. The serum titer was determined by an ELISA. The antibodies were tested by an immunodot assay with immunogold markers. With both techniques we obtained specific and relatively high-titer antibodies to clenbuterol. It was found that the antibodies obtained with the use of gold nanoparticles were not inferior in titer to those obtained to the conjugates of clenbuterol with the protein but surpassed them in specificity.
Vasilenko
O A
OA
Research Center "Nita-Farm Co.", Saratov, Russia.
Staroverov
S A
SA
Yermilov
D N
DN
Pristensky
D V
DV
Shchyogolev
S Yu
SY
Dykman
L A
LA
D000318:Adrenergic beta-Agonists / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D002682:Chinchilla; D002976:Clenbuterol / Q000276:immunology*; D004337:Drug Carriers; D004797:Enzyme-Linked Immunosorbent Assay; D017979:Gold Colloid; D011817:Rabbits; D012710:Serum Albumin, Bovine
D000318:Adrenergic beta-Agonists; D000906:Antibodies; D004337:Drug Carriers; D017979:Gold Colloid; D012710:Serum Albumin, Bovine; D002976:Clenbuterol