Quality evaluation of Yin Chen Hao Tang extract based on fingerprint chromatogram and simultaneous determination of five bioactive constituents.
A completely validated method based on HPLC coupled with photodiode array detector (HPLC-UV) was described for evaluating and controlling quality of Yin Chen Hao Tang extract (YCHTE). First, HPLC-UV fingerprint chromatogram of YCHTE was established for preliminarily elucidating amount and chromatographic trajectory of chemical constituents in YCHTE. Second, for the first time, five mainly bioactive constituents in YCHTE were simultaneously determined based on fingerprint chromatogram for furthermore controlling the quality of YCHTE quantitatively. The developed method was applied to analyze 12 batches of YCHTE samples which consisted of herbal drugs from different places of production, showed acceptable linearity, intraday (RSD <5%), interday precision (RSD <4.80%), and accuracy (RSD <2.80%). As a result, fingerprint chromatogram determined 15 representative general fingerprint peaks, and the fingerprint chromatogram resemblances are all better than 0.9996. The contents of five analytes in different batches of YCHTE samples do not indicate significant difference. So, it is concluded that the developed HPLC-UV method is a more fully validated and complete method for evaluating and controlling the quality of YCHTE.
Wang
Xijun
X
Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, China. wxj@hljucm.net
Lv
Haitao
H
Sun
Hui
H
Jiang
Xingang
X
Wu
Zeming
Z
Sun
Wenjun
W
Wang
Ping
P
Liu
Lian
L
Bi
Kaishun
K
D002138:Calibration; D002845:Chromatography / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000592:standards*; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts