Interaction of glycopolymers with human hematopoietic cells from cord blood and peripheral blood.
Polystyrene derivatives, poly[N-pvinylbenzyl-O-D-glucopyranosyl-(1-4)-D-glucoamide] (PV Maltose) and poly[N-p-vinylbenzyl-O-mannopyranosyl-(1-4)-D-glucoamide] (PV Mannose), which contain glucose and mannose moieties, respectively, have the specific binding ability with murine hematopoietic cells. In this study, we confirm the ability of these glycopolymers to interact specifically with human hematopoietic stem cells (HSCs) and mature cells derived from human cord blood (CB) and peripheral blood (PB). Using fluorescence isothiocyanate (FITC)-labeled glycopolymers, we observed that 98% to 93% of hematopoietic cells interacted very strongly with PV Mannose, and 63% of CB and 29% PB interacted with PV Maltose. Both glycopolymers bound better to cells from CB than from PB. Cytotoxic studies revealed that a 0.1 mM dose of PV Mannose induced apoptosis in 20% CB cells, in contrast to 3-5% PB cells. Furthermore, we demonstrated that all of CD34(+) HSCs of both origins bound specifically to PV Mannose, whereas 33-47% bound to PV Maltose. In addition, the majority of B cells (CD19(+)), T cells (CD3(+)), monocytes (CD14(+)), and erythrocytes (CD235a(+)) bound to PV Mannose, but a lower percentage interacted with PV Maltose. In vivo study, bone marrow, spleen, and liver tissues in NOD-SCID mice injected with PV Mannose conjugated CB, were detected PV Mannose positive hematopoietic cells. These data suggest that the use of PV Mannose and PV Maltose might be used for gene and drug delivery for hematopoietic cells and thus, may be useful in therapeutic settings.
Lee
Yong-Su
YS
College of Medicine, CHA Stem Cell Institute 606-16, Pochon CHA University, Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Republic of Korea.
Park
Keun-Hong
KH
Kim
Tea-Sik
TS
Kim
Jea-Myeoung
JM
Sohn
In-Sook
IS
Park
Jin-Ki
JK
Chang
Won-Kyong
WK
Kim
Dong-Ku
DK
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage / Q000187:drug effects; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006413:Hematopoietic System / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D008320:Maltose / Q000494:pharmacology; D008358:Mannose / Q000494:pharmacology; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D011108:Polymers / Q000378:metabolism* / Q000494:pharmacology
D011108:Polymers; D008320:Maltose; D005947:Glucose; D008358:Mannose