title: H5-type influenza virus hemagglutinin is functionally recognized by the natural killer-activating receptor NKp44.
abstract: Antiviral immune defenses involve natural killer (NK) cells. We previously showed that the NK-activating receptor NKp44 is involved in the functional recognition of H1-type influenza virus strains by NK cells. In the present study, we investigated the interaction of NKp44 and the hemagglutinin of a primary influenza virus H5N1 isolate. Here we show that recombinant NKp44 recognizes H5-expressing cells and specifically interacts with soluble H5 hemagglutinin. H5-pseudotyped lentiviral particles bind to NK cells expressing NKp44. Following interaction with target cells expressing H5, pseudotyped lentiviral particles, or membrane-associated H5, NK cells show NKp44-mediated induced activity. These findings indicate that NKp44-H5 interactions induce functional NK activation.
authors: Shraga Segal Department of Microbiology and Immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
authors: Joanna W
authors: JW
authors: Ho
authors: Oren
authors: O
authors: Hershkovitz
authors: Malik
authors: M
authors: Peiris
authors: Alon
authors: A
authors: Zilka
authors: Ahuva
authors: A
authors: Bar-Ilan
authors: Beatrice
authors: B
authors: Nal
authors: Kid
authors: K
authors: Chu
authors: Mateusz
authors: M
authors: Kudelko
authors: Yiu Wing
authors: YW
authors: Kam
authors: Hagit
authors: H
authors: Achdout
authors: Michal
authors: M
authors: Mandelboim
authors: Ralf
authors: R
authors: Altmeyer
authors: Ofer
authors: O
authors: Mandelboim
authors: Roberto
authors: R
authors: Bruzzone
authors: Angel
authors: A
authors: Porgador
mesh_terms: D002460:Cell Line; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology* / Q000821:virology; D055629:Natural Cytotoxicity Triggering Receptor 2; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; C112837:NCR2 protein, human; D055629:Natural Cytotoxicity Triggering Receptor 2; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; C109963:hemagglutinin, avian influenza A virus