Improved co-oxidation of beta-carotene to beta-ionone using xanthine oxidase-generated reactive oxygen species in a multiphasic system.
beta-Ionone, an aroma compound exhibiting flower notes, can be obtained from beta-carotene in a cooxidation system utilizing xanthine oxidase-generated reactive oxygen species (ROS). ROS have to be controlled as, although they can give rise to beta-ionone, they may also degrade it. In this work, the biotransformation of beta-carotene into beta-ionone was investigated in systems containing variable proportions of decane to extract beta-ionone before degradation. The use of 50% or 90% decane resulted in increased production yields. Tween 80, which was added to further improve the production, slightly decreased the reactivity of the medium and the extraction of beta-carotene, but increased the extraction of beta-ionone. In total, the addition of Tween 80 significantly improved the yields of conversion, which reached 34% with 50% decane and 2.5 g/L Tween 80 compared to 10% without decane and Tween 80. These results show that it is possible to control a ROS-mediated reaction by the addition of a solvent phase and by modifing the medium composition.
Ly
Mai Huong
MH
Laboratoire de Génie des Procédés Microbiologiques et Alimentaires (GPMA), ENSBANA, Université de Bourgogne, Dijon, France.
Hoang
Lan Cao
LC
Belin
Jean-Marc
JM
Waché
Yves
Y
D001709:Biotechnology / Q000379:methods; D008954:Models, Biological; D015394:Molecular Structure; D045792:Norisoprenoids / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D011136:Polysorbates / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism*; D019207:beta Carotene / Q000737:chemistry / Q000378:metabolism*
D045792:Norisoprenoids; D011136:Polysorbates; D017382:Reactive Oxygen Species; D019207:beta Carotene; C008157:beta-ionone; D014969:Xanthine Oxidase