Triplex formation-based artificial transcription factor to regulate target gene expression.
A triplex formation-based artificial transcription factor to recognize any upstream sequence of target genes was developed to regulate the target gene expression. The artificial transcription factor contains a single-stranded RNA to bind with duplex DNA of the upstream sequence of the target gene to form triplex, and an effecter domain, such as activation or repression domain, of transcription factor. Reporter beta-galactosidase activity in yeast was increased 1.5-2 times by introduction of the artificial transcription factor. The novel artificial transcription factor may be a useful tool to regulate the target gene expression and reveal unknown function of the target genes.
Torigoe
Hidetaka
H
Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo, Japan. htorigoe@ch.kagu.tus.ac.jp
Katayama
Takuma
T
Tsukamoto
Yusuke
Y
D001483:Base Sequence; D005786:Gene Expression Regulation; D017930:Genes, Reporter / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D017434:Protein Structure, Tertiary; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D001616:beta-Galactosidase / Q000235:genetics
D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D012313:RNA; D001616:beta-Galactosidase