Biological properties of single chemical-DNA adducts: a twenty year perspective.
The genome and its nucleotide precursor pool are under sustained attack by radiation, reactive oxygen and nitrogen species, chemical carcinogens, hydrolytic reactions, and certain drugs. As a result, a large and heterogeneous population of damaged nucleotides forms in all cells. Some of the lesions are repaired, but for those that remain, there can be serious biological consequences. For example, lesions that form in DNA can lead to altered gene expression, mutation, and death. This perspective examines systems developed over the past 20 years to study the biological properties of single DNA lesions.
Delaney
James C
JC
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachussetts 02139, USA.
Essigmann
John M
JM
D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018736:DNA Adducts / Q000737:chemistry* / Q000235:genetics*; D004260:DNA Repair; D006801:Humans; D007700:Kinetics; D016296:Mutagenesis; D009711:Nucleotides / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry
D018736:DNA Adducts; D009711:Nucleotides; D009841:Oligonucleotides; D004247:DNA