title: Characterizing the interaction between aptamers and human IgE by use of surface plasmon resonance.
abstract: Human immunoglobulin E (hIgE) is such an important protein, because of its involvement in allergic disease, that it is of significance to study the interactions between it and its recognizing elements. In this report an analytical strategy based on surface plasmon resonance (SPR) was developed to probe the pattern of interaction between hIgE and its recognizing molecules, including aptamers and antibodies. The affinity constants of hIgE for the antibody and the aptamer were compared first; the aptamer has more affinity than the antibody for human IgE. To study their pattern of interaction, three different binding approaches, including adding the antibody and the streptavidin-coupled aptamer to the sensing surface, were designed. The results showed that hIgE captured on the sensing surface could form a multivalent complex with the aptamer. An ELISA-like assay using the aptamer as both capture and detection probes was then developed. This work highlights an SPR method for characterizing the interaction between the protein and aptamers that is useful for study of biomolecular interaction patterns and binding properties.
authors: School of Chemistry and Chemical Engineering, Key Lab of Analytical Chemistry for Life Science (MOE), Nanjing University, 22 Hankou Road, Nanjing, 210093, China.
authors: Jinli
authors: J
authors: Wang
authors: Renji
authors: R
authors: Lv
authors: Jingjuan
authors: J
authors: Xu
authors: Danke
authors: D
authors: Xu
authors: Hongyuan
authors: H
authors: Chen
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007073:Immunoglobulin E / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D017931:DNA Primers; D007073:Immunoglobulin E