1H NMR quantitative determination of photosynthetic pigments from green beans (Phaseolus vulgaris L.).
Using 1H nuclear magnetic resonance spectroscopy (1D and 2D), the two types of photosynthetic pigments (chlorophylls, their derivatives, and carotenoids) of "green beans" (immature pods of Phaseolus vulgaris L.) were analyzed. Compared to other analytical methods (light spectroscopy or chromatography), 1H NMR spectroscopy is a fast analytical way that provides more information on chlorophyll derivatives (allomers and epimers) than ultraviolet-visible spectroscopy. Moreover, it gives a large amount of data without prior chromatographic separation.
Valverde
Juan
J
Equipe INRA de Gastronomie Moléculaire, UMR 214, (INRA, Institut des sciences du vivant et de l'environnement [AgroParisTech]), 16 rue Claude Bernard, 75005 Paris. juan.valverde@agroparistech.fr
This
Hervé
H
D002338:Carotenoids / Q000032:analysis; D002734:Chlorophyll / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D027805:Phaseolus / Q000737:chemistry*; D010788:Photosynthesis; D010860:Pigments, Biological / Q000032:analysis*
D010860:Pigments, Biological; D002734:Chlorophyll; D002338:Carotenoids