Cellulase elicitor induced accumulation of capsidiol in Capsicum annumm L. suspension cultures.
When growth-phase cell suspension cultures of Capsicum annuum were treated with cellulase-elicitor preparation at 3 microg/ml, the level of capsidiol was transiently increased in the culture media rather than in the cells reaching its maximum approx 24 h after treatment. With methyl jasmonate it took 18 h. Elicitor treatment doubled phospholiphase A(2) (PLA(2)) activity but simultaneous treatment with aristolochic acid, a PLA(2) inhibitor, inhibited sesquiterpenoid accumulation as well as PLA(2) activity. Mastoparan, a G protein activator, treatment also increased PLA(2) activity and capsidiol production. Taken together, the present study shows that induction of capsidiol production in the C. annuum is mediated by PLA(2) activation.
Ma
Choong Je
CJ
Korea Institute of Oriental Medicine, 483 EXPO St. Yuseong-Gu, Daejeon, 305-811, Korea. choongjema@kiom.re.kr
D000085:Acetates / Q000378:metabolism; D034341:Aristolochic Acids / Q000378:metabolism; D002212:Capsicum / Q000378:metabolism*; D002478:Cells, Cultured; D002480:Cellulase / Q000378:metabolism*; D002849:Chromatography, Gas; D003517:Cyclopentanes / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D054883:Oxylipins / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D064801:Phospholipase A2 Inhibitors; D054467:Phospholipases A2 / Q000378:metabolism*; D012717:Sesquiterpenes / Q000302:isolation & purification* / Q000378:metabolism*; D013997:Time Factors; D014862:Wasp Venoms / Q000378:metabolism
D000085:Acetates; D034341:Aristolochic Acids; D003517:Cyclopentanes; D036341:Intercellular Signaling Peptides and Proteins; D054883:Oxylipins; D010455:Peptides; D064801:Phospholipase A2 Inhibitors; D012717:Sesquiterpenes; D014862:Wasp Venoms; C081843:capsidiol; C037500:mastoparan; C072239:methyl jasmonate; C000228:aristolochic acid I; D054467:Phospholipases A2; D002480:Cellulase