Identification of low-molecular-weight protein (SCP1) from shark cartilage with anti-angiogenesis activity and sequence similarity to parvalbumin.
Cartilage was considered as a possible natural source of anti-angiogenesis compounds due to its known avascular nature. In this study, a low-molecular-weight protein with an anti-angiogenesis activity was isolated from shark cartilage using a mild extraction procedure. The protein was purified to homogeneity by gel filtration and electroelution techniques and its N-terminal amino acid sequence was determined. The purified protein, designated as SCP1, represented a molecular weight of 13.7 kDa, pI of 6.9-7 and its N-terminal sequence revealed sequence similarity to alpha parvalbumin family. The protein inhibited angiogenesis when subjected to microvessel sprouting of collagen-embedded rat aortic ring assay. It is suggested that SCP1 could be considered as a new angiogenesis inhibitor derived from shark cartilage.
Rabbani-Chadegani
Azra
A
Institute of Biochemistry and Biophysics, Department of Biochemistry, University of Tehran, Tehran, Iran. rabbani@ibb.ut.ac.ir
Abdossamadi
Sayeh
S
Bargahi
Afshar
A
Yousef-Masboogh
Marzeih
M
D000595:Amino Acid Sequence; D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D002356:Cartilage / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010320:Parvalbumins / Q000737:chemistry*; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012754:Sharks
D020533:Angiogenesis Inhibitors; D010320:Parvalbumins; D011506:Proteins