Growth of hairy-root cultures in various bioreactors for the production of secondary metabolites.
Agrobacterium rhizogenes-mediated hairy-root cultures are a very promising alternative to the biotechnological exploitation of plant cell cultures. Their characteristic capacity for secondary-metabolite production, inherent genetic stability reflected in stable productivity and the possibility of genetic manipulation to increase biosynthetic capacity have initiated a considerable interest, both as a fundamental research tool and as a source of valuable products. One of the most important limitations for the commercial exploitation of hairy roots is the development of technologies for large-scale culture. Though these roots have been grown in various bioreactors--stirred-tank, bubble-column, airlift or submerged, trickle-bed and nutrient mist--the question as to which of these alternatives can be successfully and economically scaled-up has yet to be definitively answered. This present minireview highlights various perspectives of hairy-root cultures, describes a comparative scale-up study and discusses various aspects of these cultures when grown in various bioreactors for the production of secondary metabolites.
Mishra
Bhartendu Nath
BN
Department of Biotechnology, Institute of Engineering and Technology, Sitapur Road, Lucknow 226021, Uttar Pradesh, India.
Ranjan
Ritu
R
D001532:Atropa belladonna / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D019149:Bioreactors; D005818:Genetic Engineering; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism / Q000382:microbiology; D012231:Rhizobium; D046509:Tissue Culture Techniques / Q000379:methods