Metabolic degradation of phenazine-1-carboxylic acid by the strain Sphingomonas sp. DP58: the identification of two metabolites.
The biotransformation of phenazine-1-carboxylic acid (PCA) by PCA-degrading strain Sphingomonas sp. DP58 yielded small quantities of metabolites and was demonstrated for the first time. The metabolites were isolated by using preparative high-performance liquid chromatography (HPLC). In addition, these were subsequently characterized by gas chromatography (GC)-mass spectrum (MS) after N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) derivatization and (1)H-nuclear magnetic resonance (NMR). They were identified as 4-hydroxy-1-(2-carboxyphenyl) azacyclobut-2-ene-2-carbonitrile (HPAEC) and 4-hydroxy-1-(2-carboxyphenyl)-2-azetidinecarbonitrile (HPAC). The two metabolites had transformational relationship between each other.
Chen
Kuaikuai
K
Key Laboratory of Microbial Metabolism, Ministry of Education, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, P.R. China.
Hu
Hongbo
H
Wang
Wei
W
Zhang
Xuehong
X
Xu
Yuquan
Y
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D010619:Phenazines / Q000378:metabolism; D020578:Sphingomonas / Q000378:metabolism*
D010619:Phenazines; C037165:1-phenazinecarboxylic acid