title: Inheritance and expression of transgenes through anther culture of transgenic hot pepper.
abstract: Anther cultures have been developed from transgenic donor peppers carrying HPT/J1-1. Eight out of sixteen plants produced from an anther culture set pepper fruits. Southern blot analysis of donors revealed two independent plants with a single copy of the integrated transgene. PCR and RT-PCR results showed the inheritance of HPT/J1-1 and expression of J1-1 in A1. All A1 progeny derived from transgenic anthers had resistance to hygromycin. They grew normally and showed similar phenotypes to the wild-type. Therefore, the use of an anther culture system coupled with genetic transformation in breeding programs will greatly facilitate the genetic improvement of pepper plants.
authors: Kumho Life and Environmental Science Laboratory & Agricultural Plant Stress Research Center, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea.
authors: Young Soon
authors: YS
authors: Kim
authors: Yong In
authors: YI
authors: Kuk
authors: Kyung-Moon
authors: KM
authors: Kim
mesh_terms: D002212:Capsicum / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D002934:Cinnamates / Q000494:pharmacology; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D012098:Reproduction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D002934:Cinnamates; D018744:DNA, Plant; D006921:Hygromycin B; C026273:hygromycin A