Evaluation of in vivo efficacy of topical formulations containing soybean extract.
In the present study it was evaluated the: (i) functional stability of the soybean extract as a raw material and dispersed in two different topical formulations, (ii) skin retention using modified Franz diffusion cells, and (iii) in vivo activity of these formulations to inhibit 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced hydrogen peroxide (H(2)O(2)) and malondialdehyde (MDA) increases in the skin of hairless mice. The physico-chemical stability was evaluated by pH, globule size and centrifugation test. Furthermore, functional stability was also evaluated by antilipoperoxidative activity. The two topical formulations were stored at 4 degrees C, 30 degrees C/60% RH and 40 degrees C/70% RH for 6 months. The evaluation of the antiperoxidative stability of soybean extract itself and incorporated in formulations did not demonstrate loss of activity by storage at 4 degrees C/6 months. During 6 months of the study in different storage conditions the formulations 1 and 2 added or not with soybean extract were stable to physico-chemical tests. The effect of antioxidant compounds detected by the inhibition of MDA formation was time-dependent for formulation 2 as detected in the skin retention study. Pretreatment with formulation 1 or 2 significantly diminished TPA-induced H(2)O(2) and MDA generation. In conclusion, the present results suggest for the first time that formulations containing soybean extract may be a topical source of antioxidant compounds that decrease oxidative damages of the skin.
Georgetti
Sandra R
SR
Department of Pharmaceutical Science, Faculty of Pharmaceutical Sciences of Ribeirao Preto - USP, Avenue Do Café s/n, CEP 14040-903, Ribeirao Preto, SP, Brazil. sangeorgetti@gmail.com
Casagrande
Rúbia
R
Verri
Waldiceu A
WA
Lopez
Renata F V
RF
Fonseca
Maria J V
MJ
D000279:Administration, Cutaneous; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002626:Chemistry, Pharmaceutical; D003879:Dermatologic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D015194:Diffusion Chambers, Culture; D004355:Drug Stability; D004356:Drug Storage; D006813:Humidity; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D018384:Oxidative Stress / Q000187:drug effects*; D010316:Particle Size; D010936:Plant Extracts / Q000494:pharmacology; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D012869:Skin Absorption; D013025:Glycine max / Q000737:chemistry; D013552:Swine; D013696:Temperature; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors
D000975:Antioxidants; D003879:Dermatologic Agents; D010936:Plant Extracts; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate