The effects of inhaled acetone on place conditioning in adolescent rats.
BACKGROUND
Acetone is an ubiquitous ingredient in many household products (e.g., glue solvents, air fresheners, adhesives, nail polish, and paint) that is putatively abused; however, there is little empirical evidence to suggest that acetone alone has any abuse liability. Therefore, we systematically investigated the conditioned response to inhaled acetone in a place conditioning apparatus.


METHODS
Three groups of male, Sprague-Dawley rats were exposed to acetone concentrations of 5000, 10,000 or 20,000 ppm for 1 h in a conditioned place preference apparatus alternating with air for 6 pairing sessions. A place preference test ensued in an acetone-free environment. To test the preference of acetone as a function of pairings sessions, the 10,000 ppm group received an additional 6 pairings and an additional group received 3 pairings. The control group received air in both compartments. Locomotor activity was recorded by infrared photocells during each pairing session.


RESULTS
We noted a dose response relationship to acetone at levels 5000-20,000 ppm. However, there was no correlation of place preference as a function of pairing sessions at the 10,000 ppm level. Locomotor activity was markedly decreased in animals on acetone-paired days as compared to air-paired days.


CONCLUSIONS
The acetone concentrations we tested for these experiments produced a markedly decreased locomotor activity profile that resemble CNS depressants. Furthermore, a dose response relationship was observed at these pharmacologically active concentrations, however, animals did not exhibit a positive place preference.
Lee
Dianne E
DE
Medical Department, Brookhaven National Laboratory, Upton, NY 11973, USA. dlee@bnl.gov <dlee@bnl.gov>
Pai
Jennifer
J
Mullapudui
Uma
U
Alexoff
David L
DL
Ferrieri
Richard
R
Dewey
Stephen L
SL
D000096:Acetone / Q000008:administration & dosage / Q000494:pharmacology*; D000280:Administration, Inhalation; D000818:Animals; D019540:Area Under Curve; D003216:Conditioning, Operant / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012997:Solvents / Q000008:administration & dosage / Q000494:pharmacology*
D012997:Solvents; D000096:Acetone