title: Live cells-based cytotoxic sensorchip fabricated in a microfluidic system.
abstract: We report here a live cells-based sensorchip fabricated in microfluidic channels in which several laminar flows were achieved. In addition, we established a cytotoxic sensor cell line, which was transfected with a green fluorescence protein (GFP) plasmid derived from the heat shock protein 70B' (HSP70B') promoter, which is induced by cytotoxic reagents. The fluorescence in the sensor cells increased in a CdCl(2) dose-dependent manner in the microfluidic channels. In this system, cytotoxic reagents can be quantitatively detected in a quick, sensitive and high-throughput manner. The combination of sensor cells and microfluidic systems will provide an important basis for the development of micro-total analysis systems (micro-TAS) technology, and can be applied to toxicology, environmental assessment and drug screening.
authors: Cell-Sensing Group, Biomaterials Center, National Institute for Materials Science, 1-1, Namiki, Tsukuba, Ibaraki 305-0044, Japan.
authors: Ken-Ichi
authors: K
authors: Wada
authors: Akiyoshi
authors: A
authors: Taniguchi
authors: Jun
authors: J
authors: Kobayashi
authors: Masayuki
authors: M
authors: Yamato
authors: Teruo
authors: T
authors: Okano
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D041681:NIH 3T3 Cells; D018675:Toxicity Tests / Q000295:instrumentation* / Q000379:methods