Antioxidant effects of the VO(IV) hesperidin complex and its role in cancer chemoprevention.
Vanadium compounds are known for a variety of pharmacological properties. Many of them display antitumoral and osteogenic effects in several cell lines. Free radicals induce the development of tumoral processes. Natural polyphenols such as flavonoids have antioxidant properties since they scavenge different free radicals. For these reasons it is interesting to investigate the effects of a new complex generated between the vanadyl(IV) cation and the flavonoid hesperidin. The complex has been synthesized and characterized by physicochemical methods. Spectroscopic analysis revealed a 1:1 stoichiometry of ligand:VO and coordination by deprotonated cis-hydroxyl groups to the disaccharide moiety of the ligand. The complex improves the superoxide dismutase (SOD)-like activity of the ligand, but the scavenging of other radicals tested does not change upon complexation. When tested on two tumoral cell lines in culture (one of them derived from a rat osteosarcoma UMR106 and the other from human colon adenocarcinoma Caco-2), the complex enhanced the antiproliferative effects of the free ligand, and this effect correlated with the morphological alterations toward apoptosis. Also, on the osteoblastic cell line the complex stimulated cell proliferation and collagen type I production at low concentrations. At higher doses the complex behaved as a cytotoxic compound for the osteoblasts.
Etcheverry
Susana Beatriz
SB
Centro de Química Inorgánica (CEQUINOR/CONICET,UNLP), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, C. Correo 962, 1900 La Plata, Argentina.
Ferrer
Evelina Gloria
EG
Naso
Luciana
L
Rivadeneira
Josefina
J
Salinas
Victoria
V
Williams
Patricia Ana María
PA
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D004578:Electron Spin Resonance Spectroscopy; D006569:Hesperidin / Q000494:pharmacology*; D006801:Humans; D009369:Neoplasms / Q000517:prevention & control*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D051381:Rats; D013055:Spectrophotometry, Infrared; D013482:Superoxide Dismutase / Q000378:metabolism; D017968:Vanadium Compounds / Q000494:pharmacology*
D016588:Anticarcinogenic Agents; D000975:Antioxidants; D017968:Vanadium Compounds; D006569:Hesperidin; D013482:Superoxide Dismutase; D000469:Alkaline Phosphatase