Neutralization of staphylococcal exotoxins in vitro by human-origin intravenous immunoglobulin.
Human-origin intravenous immunoglobulin (IVIG) collected from healthy individuals was tested for its neutralizing activity against the hemolysin, toxic shock syndrome toxin-1 (TSST-1), and enterotoxins, produced by laboratory strains of methicillin-resistant Staphylococcus aureus (MRSA). The hemolytic activity of the culture supernatant against sheep red blood cells was reduced from 100% to 5.5% relative hemolysis in the presence of 0.156 mg protein/ml of IVIG. The maximum dilution endpoint of the culture supernatant for TSST-1-mediated latex aggregation was 32-fold. This level of TSST-1 activity was reduced to eightfold dilution by 0.78 mg protein/ml of IVIG, and the latex aggregation activity of undiluted TSST-1 in the culture supernatant was inhibited by 1.56 mg protein/ml of IVIG. Similarly, the enterotoxin A-mediated latex aggregation titer appeared to be a 320-fold dilution. This toxin activity was reduced to an 80-fold dilution and below a tenfold dilution by 0.049 through 0.78 and 1.56 mg protein/ml, respectively, of IVIG. These results show that IVIG has powerful neutralizing activity against hemolysin, TSST-1, and enterotoxin A. Therefore, IVIG may be useful for passive immunization therapy in patients suffering from diseases caused by MRSA exotoxins.
Yanagisawa
Chie
C
Kitasato Research Center for Anti-infection Drugs, 5-9-1 Shirokane, Minato-Ku, Tokyo 108-0072, Japan. chieyanagisawa131@yahoo.co.jp
Hanaki
Hideaki
H
Natae
Taiji
T
Sunakawa
Keisuke
K
D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D004768:Enterotoxins / Q000235:genetics / Q000276:immunology*; D005098:Exotoxins / Q000276:immunology*; D006460:Hemolysin Proteins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D016756:Immunoglobulins, Intravenous / Q000276:immunology*; D007841:Latex Fixation Tests; D016106:Methicillin Resistance; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D018089:Superantigens / Q000235:genetics / Q000276:immunology*
D001427:Bacterial Toxins; D004768:Enterotoxins; D005098:Exotoxins; D006460:Hemolysin Proteins; D016756:Immunoglobulins, Intravenous; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal