A rapid sample pretreatment protocol: improved sensitivity in the detection of a low-abundant serum biomarker for prostate cancer.
We have developed a rapid immunoglobulin G (IgG) and a human serum albumin (HSA) depletion protocol. We depleted both HSA and IgG (> 97%) separately, and in a single procedure. The method is specific and reproducible (RSD < 1.0%), and substantially lowered the detection limit of prostate-specific antigen, a prostate cancer biomarker. The method can be applied to other biomarkers and proteomic studies. Interestingly, the depletion of HSA might not be blankly as beneficial as widely portrayed. Our study suggests the depletion of IgG to be more beneficial than albumin depletion.
Vestergaard
Mun'delanji
M
School of Material Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan. munde@jaist.ac.jp
Tamiya
Eiichi
E
D014408:Biomarkers, Tumor / Q000032:analysis*; D006063:Chorionic Gonadotropin / Q000032:analysis; D006801:Humans; D007120:Immunochemistry; D007074:Immunoglobulin G / Q000737:chemistry; D008297:Male; D009419:Nerve Tissue Proteins / Q000737:chemistry; D017430:Prostate-Specific Antigen / Q000097:blood*; D011471:Prostatic Neoplasms / Q000097:blood* / Q000175:diagnosis*; D011786:Quality Control; D012709:Serum Albumin
D014408:Biomarkers, Tumor; D006063:Chorionic Gonadotropin; C029152:G-substrate; D007074:Immunoglobulin G; D009419:Nerve Tissue Proteins; D012709:Serum Albumin; D017430:Prostate-Specific Antigen