Oligomycin A and the IPLB-LdFB insect cell line: actin and mitochondrial responses.
Oligomycin A, an inhibitor of mitochondrial ATP synthase, provokes simultaneous and different responses in IPLB-LdFB insect cell line. The oligomycin A treatment causes mitochondrial loss, increase in reactive oxygen species (ROS), destabilization/reorganization of the actin microfilaments and, finally, autophagic cell death. We speculate that oligomycin A affects the mitochondria and that the impairment of these organelles leads to the generation of ROS in quantities that exceed the antioxidant capacity of the cell. This in turn would lead to a feedback loop of increased mitochondrial impairment, amplification of ROS production and the removal of damaged organelles through autophagy.
Tettamanti
Gianluca
G
Department of Structural and Functional Biology, University of Insubria, Via J.H. Dunant 3, 21100 Varese, Italy.
Malagoli
Davide
D
Ottaviani
Enzo
E
de Eguileor
Magda
M
D000199:Actins / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D009036:Moths; D009840:Oligomycins / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D000199:Actins; D009840:Oligomycins; D017382:Reactive Oxygen Species; C031004:oligomycin A