Qualitative evidence of Ri specific IgG-synthesis in the cerebrospinal fluid from patients with paraneoplastic neurological syndromes.
The presence of Ri-specific oligoclonal IgG bands in the CSF was investigated in five patients with paraneoplastic anti-Ri associated neurological syndromes (PNS) and six controls. In 4/5 CSF samples reactivity of IgG bands with recombinant Ri antigen was found using isoelectrofocusing combined with affinity blotting; in one patient with absence of oligoclonal bands of total IgG in CSF Ri-specific oligoclonal bands were detected with the same sample, indicating a higher sensitivity of Ri-specific affinity blotting as compared to affinity blotting with anti-human IgG antibodies. Our results confirm previous studies demonstrating IgG synthesis against onconeuronal antigens by intrathecal B-cell clones in PNS and extend this observation to patients with anti-Ri syndrome. The pathogenic relevance of these antibodies, however, is further challenged by the finding that specific intrathecal IgG synthesis might not be a prerequisite of CNS involvement, because it was missed in one of our patients.
Jarius
S
S
Institute of Clinical Neuroimmunology, Ludwig Maximilians University, Munich, Germany.
Stich
O
O
Rasiah
C
C
Voltz
R
R
Rauer
S
S
D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000276:immunology*; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000276:immunology*; D000090682:Neuro-Oncological Ventral Antigen; D043025:Oligoclonal Bands / Q000134:cerebrospinal fluid*; D020364:Paraneoplastic Polyneuropathy / Q000134:cerebrospinal fluid*; D016601:RNA-Binding Proteins / Q000276:immunology*
D000951:Antigens, Neoplasm; D009419:Nerve Tissue Proteins; D000090682:Neuro-Oncological Ventral Antigen; D043025:Oligoclonal Bands; D016601:RNA-Binding Proteins