title: Effects of phenolic monomers on the enzymes activities and volatile fatty acids production of Neocallimastix frontalis B9.
abstract: The effects of phenolic monomers (i.e. rho-coumaric acid, ferulic acid, rho-hydroxybenzaldehyde and vanillin) on the enzymes and fermentation activities of Neocallimastix frontalis B9 grown in ball-milled filter paper and guinea grass media were studied. The enzymes studied were carboxymethylcellulase (CMCase), filterpaperase (FPase), xylanase and beta-glucosidase. At 96 h of incubation, N. frontalis grown in ball-milled filter paper medium produced comparable xylanase and CMCase activities (0.41, 0.5 micromol/min/mg protein) while in guinea grass medium, N. frontalis produced higher xylanase activity than that of CMCase activity (2.35, 0.05 micromol/min/mg protein). The other enzymes activities were low. When N. frontalis was grown in ball-milled filter paper medium, only acetic acid was produced. However, when grown in guinea grass medium, the major end-product was acetate, but propionic, butyric and isovaleric were also produced in lesser amount. Vanillin showed the least inhibitory effects to enzyme activities of N. frontalis B9 grown in both ball-milled filter paper and guinea grass media. For total volatile fatty acid production, all phenolic monomers showed inhibitory effects, but rho-coumaric and ferulic acids were the stronger inhibitors than rho-hydroxybenzaldehyde and vanillin.
authors: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, Malaysia. zuhainis@biotech.upm.edu.my
authors: Wan
authors: W
authors: Zuhainis Saad
authors: Norhani
authors: N
authors: Abdullah
authors: Abdul Razak
authors: AR
authors: Alimon
authors: Ho
authors: H
authors: Yin Wan
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D000935:Antifungal Agents / Q000494:pharmacology*; D002087:Butyrates / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000096:biosynthesis*; D045782:Hemiterpenes; D020088:Neocallimastix / Q000187:drug effects* / Q000378:metabolism*; D010421:Pentanoic Acids / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D011422:Propionates / Q000378:metabolism; D014995:Xylosidases / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D002087:Butyrates; D005232:Fatty Acids, Volatile; D045782:Hemiterpenes; D010421:Pentanoic Acids; D010636:Phenols; D011422:Propionates; C008216:isovaleric acid; D014995:Xylosidases; D001617:beta-Glucosidase; D002480:Cellulase; C019826:carboxymethylcellulase; D019342:Acetic Acid