Genomic and phylogenetic analysis of two novel bovine papillomaviruses, BPV-9 and BPV-10.
Eight bovine papillomavirus (BPV) types, BPV-1-8, have been classified, based on genome nucleotide sequence similarities, in the genera Deltapapillomavirus (BPV-1 and -2), Epsilonpapillomavirus (BPV-5 and -8), Xipapillomavirus (BPV-3, -4 and -6) and an unassigned genus (BPV-7). We report here the complete genome sequence of two new BPV types isolated from separate epithelial squamous papilloma lesions on cattle teats. The genomes are 7,303 and 7,399 bp in length, respectively, and both have genetic organization and consensus motifs typical of papillomaviruses. A neighbour-joining phylogenetic tree revealed that both viruses cluster with BPV-3, -4 and -6. Nucleotide sequence identities of the BPV L1 major capsid protein of these two new BPVs with BPV-3, their closest relative, are 74.2 and 71.2 %, respectively. These results suggest that both viruses are new BPV types in the genus Xipapillomavirus, and they are designated BPV-9 and BPV-10.
Hatama
Shinichi
S
Hokkaido Research Station, National Institute of Animal Health, 4 Hitsujigaoka, Toyohira, Sapporo 062-0045, Japan.
Nobumoto
Kiyoko
K
Tokachi Livestock Hygiene Service Center, 59-6 Kisen, Kawanishi, Obihiro 089-1182, Japan.
Kanno
Toru
T
Hokkaido Research Station, National Institute of Animal Health, 4 Hitsujigaoka, Toyohira, Sapporo 062-0045, Japan.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017124:Conserved Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D052687:Deltapapillomavirus / Q000145:classification* / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016385:TATA Box
D004279:DNA, Viral