[Effects of 50 Hz magnetic fields exposure on secretion of primary human villous trophoblasts].
OBJECTIVE
To explore the possible effects of 50 Hz magnetic fields (MF) exposure on HCG and progesterone secretion of human villous trophoblasts in vitro.


METHODS
The trophoblasts were isolated from human villus by trypsin digestion and incubated in DMEM medium. Then the trophoblasts were exposed to 0.2 mT, 0.4 mT 50 Hz MF for 6 h, 12 h, 24 h, 48 h and 72 h, respectively. Each exposure group was matched to one control group which was from the same villus and cultured with the same condition except the 50 Hz MF exposure. The concentration of human chorionic gonadotropin (HCG) and progesterone in the culture medium was detected by electrochemiluminescence immunoassay. Statistical significance of differences between means was determined by one way-ANOVA with P < 0.05 considered significant.


RESULTS
Exposure of trophoblasts to 50 Hz MF at 0.2 mT intensity within 72 h did not affect the secretion level of HCG and progesterone (compared with blank control, P > 0.05). There was also no significant change of the secretion level of HCG and progesterone when trophoblasts were exposed to 0.4 mT 50 Hz MF within 48 h (compared with blank control, P > 0.05). However, 50 Hz MF inhibited the HCG and progesterone secretion significantly with exposure for 72 h (compared with blank control, P < 0.05).


CONCLUSIONS
The exposure to 50 Hz MF for long period could inhibit trophoblasts excreting the HCG and progesterone, and the threshold intensity may be between 0.2 mT and 0.4 mT.
Sun
Wen-jun
WJ
Bioelectromagnetics Key Lab, Zhejiang University, Hangzhou 310058, China. sunwj@zju.edu.cn
Pan
Yong-miao
YM
Fu
Yi-ti
YT
Sun
Hui-lan
HL
Lu
De-qiang
DQ
Zhang
Dan
D
Jiang
Hua
H
D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000378:metabolism; D005260:Female; D006801:Humans; D060526:Magnetic Fields / Q000009:adverse effects*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism; D014327:Trophoblasts / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D011374:Progesterone