Inheritance and expression of transgenes through anther culture of transgenic hot pepper.
Anther cultures have been developed from transgenic donor peppers carrying HPT/J1-1. Eight out of sixteen plants produced from an anther culture set pepper fruits. Southern blot analysis of donors revealed two independent plants with a single copy of the integrated transgene. PCR and RT-PCR results showed the inheritance of HPT/J1-1 and expression of J1-1 in A1. All A1 progeny derived from transgenic anthers had resistance to hygromycin. They grew normally and showed similar phenotypes to the wild-type. Therefore, the use of an anther culture system coupled with genetic transformation in breeding programs will greatly facilitate the genetic improvement of pepper plants.
Kim
Young Soon
YS
Kumho Life and Environmental Science Laboratory & Agricultural Plant Stress Research Center, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea.
Kuk
Yong In
YI
Kim
Kyung-Moon
KM
D002212:Capsicum / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D002934:Cinnamates / Q000494:pharmacology; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D012098:Reproduction; D020133:Reverse Transcriptase Polymerase Chain Reaction
D002934:Cinnamates; D018744:DNA, Plant; D006921:Hygromycin B; C026273:hygromycin A