Congenicity and genetic polymorphism in cloned lines derived from a single isolate of a rodent malaria parasite.
Many of the most commonly studied lines of the rodent malaria parasite Plasmodium yoelii yoelii originated from a single parasite isolate designated 17X. Amongst these lines, however, are parasites that exhibit variation in genotype and phenotype (e.g. growth rate). We describe here the results of a comparative genetic analysis between cloned lines of 17X that differ in growth rate, using nucleotide sequences of specific genes and patterns of genome-wide amplified fragment length polymorphism (AFLP). Our findings indicate that the original stock of 17X comprises two unrelated genotypes. Genotype-1 is represented by parasites with a slow growth phenotype (e.g. 17X (NIMR)) and a fast growth phenotype (e.g. 17XYM). Within this genotype, there are also genomic differences manifest as a small number of AFLP bands that differentiate the fast- and slow-growing lines from each other. The other genotype, genotype-2, is represented only by parasites with a slow growth phenotype (e.g. 17XA).
Pattaradilokrat
Sittiporn
S
Institute of Immunology and Infection Research, School of Biological Sciences, The University of Edinburgh, Ashworth Laboratories, Edinburgh, UK. s0343527@sms.ed.ac.uk
Cheesman
Sandra J
SJ
Carter
Richard
R
D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010967:Plasmodium yoelii / Q000235:genetics* / Q000502:physiology; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan