title: Synthesis of modified DNA by PCR with alkyne-bearing purines followed by a click reaction.
abstract: Alkyne-bearing deazapurine triphosphates were prepared and successfully incorporated into DNA using the polymerase chain reaction (PCR). The obtained alkyne-labeled DNA was successfully used in a click reaction with galactose azide.
authors: Department of Chemistry, Ludwig-Maximilians University Munich, Butenandtstr. 13, D-81377 Munich, Germany.
authors: Philipp M E
authors: PM
authors: Gramlich
authors: Christian T
authors: CT
authors: Wirges
authors: Johannes
authors: J
authors: Gierlich
authors: Thomas
authors: T
authors: Carell
mesh_terms: D001386:Azides / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005690:Galactose / Q000031:analogs & derivatives / Q000737:chemistry; D015394:Molecular Structure; D016133:Polymerase Chain Reaction; D011685:Purine Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D001386:Azides; D011685:Purine Nucleotides; D004247:DNA; D005690:Galactose