Production of L-phenylacetylcarbinol by microbial transformation in polyethylene glycol-induced cloud point system.
Microbial transformation of benzaldehyde into L: -phenylacetylcarbinol by whole cell Saccharomyces cerevisiae has been carried out in a novel polyethylene glycol (PEG)-induced cloud point system. The system is composed of 80 g PEG 20,000, 75 ml Triton X-100, 20 g peptone, 10 g yeast extract, 25 g glucose, 1 g MgSO(4).7H(2)O, 0.05 g CaCl(2).2H(2)O, 35 g Na(2)HPO(4).12H(2)O, and 10.7 g citric acid per liter of tap water. The microbial transformation is conducted at 0.6 ml of acetaldehyde (35% volume content), 0.9 ml of benzaldehyde, and 7 g of wet cell per 100 ml of the PEG-induced cloud point system. Under the conditions, a relatively longer-term bioactivity of whole cell microorganism in the PEG-induced cloud point system has been achieved. A fed-batch microbial transformation process with a discrete addition of glucose and substrate gets a high final product concentration of about 8 g/l.
Zhang
Wenzhi
W
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
Wang
Zhilong
Z
Li
Wei
W
Zhuang
Baohua
B
Qi
Hanshi
H
D000079:Acetaldehyde / Q000378:metabolism; D000096:Acetone / Q000031:analogs & derivatives* / Q000378:metabolism; D001547:Benzaldehydes / Q000378:metabolism*; D001711:Biotransformation; D003470:Culture Media / Q000737:chemistry; D005947:Glucose / Q000378:metabolism; D011092:Polyethylene Glycols / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D001547:Benzaldehydes; D003470:Culture Media; D000096:Acetone; D011092:Polyethylene Glycols; C035293:1-hydroxy-1-phenyl-2-propanone; D000079:Acetaldehyde; D005947:Glucose; C032175:benzaldehyde