title: Up-regulation of ADM and SEPX1 in the lymphoblastoid cells of patients in monozygotic twins discordant for schizophrenia.
abstract: The contribution of genetic factors to schizophrenia is well established and recent studies have indicated several strong candidate genes. However, the pathophysiology of schizophrenia has not been totally elucidated yet. To date, studies of monozygotic twins discordant for schizophrenia have provided insight into the pathophysiology of this illness; this type of study can exclude inter-individual variability and confounding factors such as effects of drugs. In this study we used DNA microarray analysis to examine the mRNA expression patterns in the lymphoblastoid (LB) cells derived from two pairs of monozygotic twins discordant for schizophrenia. From five independent replicates for each pair of twins, we selected five genes, which included adrenomedullin (ADM) and selenoprotein X1 (SEPX1), as significantly changed in both twins with schizophrenia. Interestingly, ADM was previously reported to be up-regulated in both the LB cells and plasma of schizophrenic patients, and SEPX1 was included in the list of genes up-regulated in the peripheral blood cells of schizophrenia patients by microarray analysis. Then, we performed a genetic association study of schizophrenia in the Japanese population and examined the copy number variations, but observed no association. These findings suggest the possible role of ADM and SEPX1 as biomarkers of schizophrenia. The results also support the usefulness of gene expression analysis in LB cells of monozygotic twins discordant for an illness.
authors: Laboratory for Molecular Dynamics of Mental Disorders, RIKEN Brain Science Institute, Wako-shi, Saitama, Japan.
authors: Chihiro
authors: C
authors: Kakiuchi
authors: Mizuho
authors: M
authors: Ishiwata
authors: Shinichiro
authors: S
authors: Nanko
authors: Norio
authors: N
authors: Ozaki
authors: Nakao
authors: N
authors: Iwata
authors: Tadashi
authors: T
authors: Umekage
authors: Mamoru
authors: M
authors: Tochigi
authors: Kazuhisa
authors: K
authors: Kohda
authors: Tsukasa
authors: T
authors: Sasaki
authors: Akira
authors: A
authors: Imamura
authors: Yuji
authors: Y
authors: Okazaki
authors: Tadafumi
authors: T
authors: Kato
mesh_terms: D053607:Adrenomedullin / Q000096:biosynthesis / Q000235:genetics*; D000328:Adult; D016022:Case-Control Studies; D002478:Cells, Cultured; D005260:Female; D018628:Gene Dosage; D020869:Gene Expression Profiling; D006801:Humans; D016131:Lymphocyte Subsets / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051150:Methionine Sulfoxide Reductases / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D012559:Schizophrenia / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051140:Selenoproteins / Q000096:biosynthesis / Q000235:genetics*; D014430:Twins, Monozygotic / Q000235:genetics* / Q000523:psychology; D015854:Up-Regulation / Q000502:physiology*
chemical_list: C507862:ADM protein, human; D051140:Selenoproteins; D053607:Adrenomedullin; D051150:Methionine Sulfoxide Reductases