Antibacterial activity of essential oils on the growth of Staphylococcus aureus and measurement of their binding interaction using optical biosensor.
Antibacterial activity of essential oils (Tea tree, Chamomile, Eucalyptus) on Staphylococcus aureus growth was evaluated as well as the essential oil-loaded alginate beads. The binding interactions between the cell and the essential oils were measured using an optical biosensor. The antibacterial activity of the essential oils to the cell was evaluated with their binding interaction and affinity. The antibacterial activity appeared in the order of Tea Tree> Chamomile>Eucalyptus, in comparison of the inhibition effects of the cell growth to the essential oils. The association rate constant and affinity of the cell binding on Tea Tree essential oil were 5.0 x 10(-13) ml/(CFU-s) and 5.0 x 10(5) ml/CFU, respectively. The affinity of the cell binding on Tea Tree was about twice higher than those on the other essential oils. It might be possible that an effective antibacterial activity of Tea Tree essential oil was derived from its strong adhesive ability to the cell, more so than those of the other essential oils.
Chung
Kyong-Hwan
KH
Center for Functional Nano Fine Chemicals, Chonnam National University, Gwangju 500-757, Korea.
Yang
Ki-Sook
KS
Kim
Jin
J
Kim
Jin-Chul
JC
Lee
Ki-Young
KY
D000464:Alginates / Q000008:administration & dosage; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015374:Biosensing Techniques / Q000379:methods*; D020723:Glucuronic Acid / Q000008:administration & dosage; D006603:Hexuronic Acids / Q000008:administration & dosage; D057927:Hydrophobic and Hydrophilic Interactions; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects*
D000464:Alginates; D000900:Anti-Bacterial Agents; D006603:Hexuronic Acids; D009822:Oils, Volatile; D020723:Glucuronic Acid