title: MyD88 but not TLR2, 4 or 9 is essential for IL-12 induction by lactic acid bacteria.
abstract: Although lactic acid bacteria (LAB) affect the immune system, for example, having an anti-allergic effect, little is known about the actual mechanisms of immune modulation. Toll-like receptors (TLRs) recognize conserved microbial molecular patterns, and are presumed to be involved in the recognition of LAB. However, there are few detailed reports examining the relationships between TLR and LAB. We measured here production of IL-12, a cytokine considered to play an important role in anti-allergic effects, induced by Lactobacillus paracasei strain KW3110 and other typical LAB by cells from TLR2-, TLR4-, TLR9- and myeloid differentiation factor 88 (MyD88)-deficient mice. Unexpectedly, similar cytokine production from wild-type and TLR2-, 4- and 9-deficient mice was observed. In contrast, cells from MyD88-deficient mice failed to respond to stimulation with LAB. It is therefore concluded that although LAB, including strain KW3110, are not likely to be recognized by TLR2, 4 or 9, MyD88 is essential for the response to these bacteria.
authors: Central Laboratories for Frontier Technology, Kirin Holdings Company Ltd., Yokohama, Japan. Shintaro_Ichikawa@kirin.co.jp
authors: Shintaro
authors: S
authors: Ichikawa
authors: Rei
authors: R
authors: Fujii
authors: Daisuke
authors: D
authors: Fujiwara
authors: Yutaka
authors: Y
authors: Komiyama
authors: Tsuneyasu
authors: T
authors: Kaisho
authors: Masahiro
authors: M
authors: Sakaguchi
authors: Yutaka
authors: Y
authors: Konishi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018664:Interleukin-12 / Q000096:biosynthesis*; D007778:Lactobacillus / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000276:immunology*; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000276:immunology*; D051217:Toll-Like Receptor 9 / Q000235:genetics / Q000276:immunology*
chemical_list: D053594:Myeloid Differentiation Factor 88; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051217:Toll-Like Receptor 9; D018664:Interleukin-12