Fabrication and performance of poly(methyl methacrylate) microfluidic chips with fiber cores.
In this work, a piece of glass fiber was inserted into the channel of a poly(methyl methacrylate) (PMMA) electrophoresis microchip to enhance the electroosmotic flow (EOF) and the separation efficiency. The EOF value of the glass fiber-containing microchannel at pH 8.2 was determined to be 4.17 x 10(-4)cm2 V(-1)s(-1). The performance of the new microchip was demonstrated by its ability to separate and detect three purines coupled with end-column amperometric detection. In addition, a piece of trypsin-immobilized glass fiber was inserted into the channel of a PMMA microchip to fabricate a core-changeable microfluidic bioreactor that can be regenerated by changing the fiber. The in-channel fiber bioreactor has been coupled with matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the digestion and peptide mapping of bovine serum albumin and myoglobin.
Fan
Huizhi
H
School of Pharmacy & Department of Chemistry, Fudan University, Shanghai 200032, China.
Chen
Zhi
Z
Zhang
Luyan
L
Yang
Pengyuan
P
Chen
Gang
G
D053841:Electroosmosis; D046209:Electrophoresis, Microchip / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010455:Peptides / Q000302:isolation & purification; D019904:Polymethyl Methacrylate / Q000737:chemistry*
D010455:Peptides; D019904:Polymethyl Methacrylate