Perspectives on microbial cell surface display in bioremediation.
The display of heterologous proteins on the microbial cell surface by means of recombinant DNA biotechnologies has emerged as a novel approach for bioremediation of contaminated sites. Both bacteria and yeasts have been investigated for this purpose. Cell surface expression of specific proteins allows the engineered microorganisms to transport, bio-accumulate and/or detoxify heavy metals as well as to degrade xenobiotics. These otherwise would not be taken up and transformed by the microbial cell. This review focuses on the application of cell surface displays for the enhanced bio-accumulation of heavy metals by metal binding proteins. It also reviews the biodegradation of xenobiotics by enzymes/proteins expressed on microbial cell surfaces.
Saleem
M
M
UFZ, Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoser Str. 15, D-04318 Leipzig, Germany. msaleemshani@gmail.com
Brim
H
H
Hussain
S
S
Arshad
M
M
Leigh
M B
MB
Zia-ul-Hassan
D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D005658:Fungi / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D019216:Metals, Heavy / Q000302:isolation & purification / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D015262:Xenobiotics / Q000302:isolation & purification / Q000378:metabolism*
D008565:Membrane Proteins; D019216:Metals, Heavy; D011994:Recombinant Proteins; D015262:Xenobiotics