title: The assessment of a rapid noninvasive immunochromatographic assay test for fecal lactoferrin in patients with suspected inflammation of the ileal pouch.
abstract: OBJECTIVE
  Pouchitis is a common complication after ileal pouch-anal anastomosis. Diagnosis is based on the Pouch Disease Activity Index, which comprises clinical symptoms, endoscopic appearance, and histologic confirmation. A Pouch Disease Activity Index > or = 7 confirms pouchitis. Fecal lactoferrin is a marker of intestinal inflammation, which can aid in the diagnosis of pouchitis. The IBD EZ VUE test is a simple, rapid, noninvasive test for fecal lactoferrin. Our goal was to study the sensitivity and specificity of this test in the diagnosis of pouchitis.
  
  
  METHODS
  Consecutive patients with pouch dysfunction were recruited from October 2005 to July 2006. A fecal sample was collected before calculation of the Pouch Disease Activity Index. An IBD EZ VUE test was performed on each fecal sample and the results correlated with the diagnosis of pouchitis to calculate sensitivity and specificity of the IBD EZ VUE test.
  
  
  RESULTS
  There were 32 patients (21 healthy and 11 inflamed pouches). The IBD EZ VUE test was positive in 14 patients. It had a sensitivity of 100 percent and a specificity of 86 percent in diagnosing pouchitis. The positive predictive value was 76 percent. There were three false-positive results.
  
  
  CONCLUSIONS
  The IBD EZ VUE test is a sensitive method that may remove the need for invasive pouch investigations and lead to greater confidence when antibiotic therapy is commenced. Further investigations may be reserved for those patients who have a positive lactoferrin test and fail to respond to antibiotic treatment.
authors: Department of Colorectal Surgery, Leeds General Infirmary, Leeds, United Kingdom.
authors: Michael
authors: M
authors: Lim
authors: Simon
authors: S
authors: Gonsalves
authors: Dinesh
authors: D
authors: Thekkinkattil
authors: Shahid
authors: S
authors: Seedat
authors: Paul
authors: P
authors: Finan
authors: Peter
authors: P
authors: Sagar
authors: Dermot
authors: D
authors: Burke
mesh_terms: D000328:Adult; D000714:Anastomosis, Surgical / Q000009:adverse effects*; D005243:Feces / Q000737:chemistry*; D005260:Female; D006801:Humans; D007781:Lactoferrin / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D019449:Pouchitis / Q000175:diagnosis*; D011446:Prospective Studies; D012680:Sensitivity and Specificity
chemical_list: D007781:Lactoferrin