FGF-2 signaling induces downregulation of TAZ protein in osteoblastic MC3T3-E1 cells.
Transcriptional coactivator with PDZ-binding motif (TAZ) protein is a coactivator of Runx2 and corepressor of PPARgamma. It also induces differentiation of mesenchymal cells into osteoblasts. In this study, we found that FGF-2, which inhibits bone mineralization and stimulates cell proliferation, reduced the TAZ protein expression level in osteoblast-like cells, MC3T3-E1. This reduction was recovered by removing FGF-2 from the culture medium, which also restored the osteoblastic features of MC3T3-E1 cells. Furthermore, FGF-2-induced reduction of TAZ is blocked by a SAPK/JNK-specific inhibitor. These findings suggest that the expression of TAZ protein is involved in osteoblast proliferation and differentiation. This may help elucidate the discrepancies in the effect of FGF-2 and contribute to the understanding of FGF/FGFR-associated craniosynostosis syndrome etiology and treatment.
Eda
Homare
H
Department of Biochemistry, The Jikei University School of Medicine, 3-25-8, Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan.
Aoki
Katsuhiko
K
Marumo
Keishi
K
Fujii
Katsuyuki
K
Ohkawa
Kiyoshi
K
D016475:3T3 Cells; D000217:Acyltransferases; D000818:Animals; D002454:Cell Differentiation; D015536:Down-Regulation; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
D011506:Proteins; D014157:Transcription Factors; D016222:Fibroblast Growth Factor 2; D000217:Acyltransferases; C486363:tafazzin protein, mouse