Azospirillum brasilense Sp7 produces an outer-membrane lectin that specifically binds to surface-exposed extracellular polysaccharide produced by the bacterium.
Azospirillum sp promotes the growth of many important crop plants. We demonstrated lectin binding activity in outer-membrane protein extracts of A. brasilense Sp7 by hemagglutination assays. The lectin specifically recognised the exopolysaccharide (EPS) produced by aggregated cells. Affinity chromatography using EPS-Sepharose was used to identify a 67 kDa outer-membrane lectin (OML) that recognised a binding region in the extracellular polysaccharide. Results show the specific recognition and binding between EPS and OML. The potential relationship between cell-to-cell aggregation and the OML-EPS interaction is discussed.
Mora
Paola
P
Cátedra de Bioquímica, Departamento de Biociencias, Facultad de Química, CC 1157, Montevideo, Uruguay.
Rosconi
Federico
F
Franco Fraguas
Laura
L
Castro-Sowinski
Susana
S
D001089:Arabinose / Q000378:metabolism; D015806:Azospirillum brasilense / Q000378:metabolism*; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D006385:Hemagglutination Inhibition Tests; D037102:Lectins / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D011485:Protein Binding; D013379:Substrate Specificity
D001425:Bacterial Outer Membrane Proteins; D037102:Lectins; D011135:Polysaccharides, Bacterial; D001089:Arabinose