Establishment of hybrid cell lines producing monoclonal antibodies to a synthetic peptide from the E1 region of the hepatitis C virus.
We aimed at establishing hybridoma cells secreting monoclonal antibodies (mAbs) against E1 synthetic peptide of HCV. BALB/c mice were immunized with HCV E1-synthetic peptide (GHRMAWDMM) and its spleenocytes were fused with the P3NS1 myeloma cell line. Two highly reactive and specific mAbs (10C7 IgG2b mAb, and 10B2 IgG1 mAb) were generated. The target HCV E1 antigen was identified at approximately 38 kDa in serum of infected individuals. A newly developed ELISA detected the target antigen in 90% of sera from HCV RNA infected individuals with a specificity of 84%. So, the generated mAbs may provide promising probes for serodiagnosis of HCV infection.
Tabll
Ashraf A
AA
Department of Biomedical Technology, National Research Center, Cairo, Egypt. ashraftabll@yahoo.com
Khalil
Samy B
SB
El-Shenawy
Reem M
RM
Esmat
Gamal
G
Helmy
Amr
A
Attallah
Abdel Fattah
AF
El-Awady
Mostafa K
MK
D000293:Adolescent; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006526:Hepatitis C / Q000175:diagnosis*; D018936:Hepatitis C Antigens / Q000097:blood / Q000737:chemistry / Q000276:immunology; D006801:Humans; D006825:Hybridomas; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D012698:Serologic Tests; D014759:Viral Envelope Proteins / Q000097:blood* / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; C087035:E1 protein, Hepatitis C virus; D018936:Hepatitis C Antigens; D010455:Peptides; D014759:Viral Envelope Proteins