Expression of the barley yellow dwarf virus-GAV movement protein and its detection in the infected and transgenic plants.
Movement proteins (MPs) that facilitate virus movement in the plants were identified in a number of plant viruses. In this study, full-length MP gene of the Chinese isolate Barley yellow dwarf virus-GAV (BYDV-GAV) was cloned and expressed in Escherichia coli. About 32% of the expressed MP was soluble providing the concentration of isopropyl-beta-D-galactopyranoside (IPTG), time of the induction, temperature and shaking speed were optimized. The soluble MP was purified using nickel-affinity column. Immune serum prepared against purified MP was used for the detection of MP in the BYDV-GAV infected leaves of oat and in the leaves of transgenic wheat plants expressing the full-length and truncated MP gene.
Xie
J
J
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Wang
X
X
Liu
Y
Y
Peng
Y
Y
Zhou
G
G
D018554:Avena / Q000737:chemistry / Q000821:virology; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D017793:Luteovirus / Q000235:genetics*; D018515:Plant Leaves / Q000737:chemistry / Q000821:virology; D053830:Plant Viral Movement Proteins / Q000096:biosynthesis* / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D014908:Triticum / Q000737:chemistry / Q000235:genetics
D053830:Plant Viral Movement Proteins; D011994:Recombinant Proteins