title: Effects of Astragalus polysaccharides (APS) on the expression of immune response genes in head kidney, gill and spleen of the common carp, Cyprinus carpio L.
abstract: For fish immune defences, cytokines and anti-microbial peptides (lysozyme) in circulating system play important roles. In the present study, the effects of Astragalus polysaccharides (APS) injection on gene expression of interleukin 1beta (IL-1beta), tumor necrosis factoralpha (TNF-alpha) and lysozyme-C in the head kidney, gill and spleen of common carp were determined using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). After injection of APS, IL-1beta mRNA level increased in a dose-dependent manner in the head kidney, while no significant changes were found in the gill and spleen. High dose of APS up regulated TNF-alpha transcription in the gill and spleen, while TNF-alpha mRNA level decreased significantly in the head kidney of low dose of APS. Lysozyme-C mRNA levels were up regulated in the gill of low dose of APS and spleen of middle dose of APS. No effect of the APS on lysozyme-C expression was observed in head kidney. These results constitute a first step toward the understanding of APS effect on cytokines and immune-related gene expression in different organs of common carp.
authors: Immunology and Reproduction Biology Laboratory, Medical School, Nanjing University, Nanjing, 210093, China.
authors: Chuntao
authors: C
authors: Yuan
authors: Xuping
authors: X
authors: Pan
authors: Yi
authors: Y
authors: Gong
authors: Aijun
authors: A
authors: Xia
authors: Guanghong
authors: G
authors: Wu
authors: Jianqing
authors: J
authors: Tang
authors: Xiaodong
authors: X
authors: Han
mesh_terms: D000818:Animals; D027885:Astragalus propinquus / Q000276:immunology*; D002347:Carps / Q000276:immunology* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology*; D005802:Genes, MHC Class II; D005880:Gills / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D007274:Injections, Intraperitoneal; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000235:genetics; D007668:Kidney / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D009113:Muramidase / Q000096:biosynthesis / Q000235:genetics; D011134:Polysaccharides / Q000008:administration & dosage* / Q000276:immunology; D013154:Spleen / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
chemical_list: D053583:Interleukin-1beta; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D009113:Muramidase