[Cloning of human bone morphogenetic protein-2 gene and the construction of its eukaryotic expression vector].
OBJECTIVE
To clone human bone morphogenetic protein-2 (hBMP2) gene and construct its eukaryotic expression vector pcDNA3.1 -hBMP2.


METHODS
Human BMP2 gene was amplified by RT-PCR method from human osteosarcoma cells and constructed into eukaryotic expression vector pcDNA3.1-hBMP2. The gene in the vector pcDNA3.1-hBMP2 was identified by PCR amplification, enzyme digestion and DNA sequencing.


RESULTS
The cloned DNA was confirmed to be hBMP-2 gene.


CONCLUSIONS
In this study, hBMP2 gene is successfully cloned and its eukaryotic expression vector pcDNA3.1-hBMP2 is constructed, which provides the foundation of using BMP2 gene therapy to accelerate new bone formation in distraction osteogenesis.
Zhou
Nuo
N
Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guangxi Medical University, Nanning 530021, China.
Huang
Xuan-ping
XP
Liao
Ni
N
Wei
Shan-liang
SL
Liang
Fei-xin
FX
Mai
Hua-ming
HM
D055396:Bone Morphogenetic Protein 2; D019976:Cloning, Organism; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014162:Transfection
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2