Stepwise functionalization of ribonucleopeptides: optimization of the response of fluorescent ribonucleopeptide sensors for ATP.
A stable complex of a peptide and RNA, ribonucleopeptide (RNP), provides a new framework to construct a macromolecular receptor for small molecules. The RNP receptor functionalized by a fluorophore-labeled Rev peptide exerts an optical signal associated with the ligand binding events. Replacing the Rev peptide of the ATP-binding RNP with a fluorophore-modified Rev peptide affords a fluorescent ATP sensor.
Hasegawa
Tetsuya
T
Institute of Advanced Energy, Kyoto University, Uji, Kyoto, Japan.
Hagihara
Masaki
M
Fukuda
Masatora
M
Morii
Takashi
T
D000255:Adenosine Triphosphate / Q000032:analysis*; D015374:Biosensing Techniques; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D012261:Ribonucleoproteins / Q000138:chemical synthesis / Q000737:chemistry*
D005456:Fluorescent Dyes; D010455:Peptides; C503402:Rev peptide; D012261:Ribonucleoproteins; D000255:Adenosine Triphosphate