Live cell approaches for studying kinetochore-microtubule interactions in Drosophila.
Kinetochores are essential for the proper positioning, movement and segregation of chromosomes on spindle microtubules. Live cell analyses of kinetochore movements on the spindle provide an important tool for dissecting the molecular machinery underlying kinetochore-based chromosome motility. Here, we describe contemporary techniques for studying and manipulating kinetochore function in live Drosophila syncytial blastoderm-stage embryos and S2 cells.
Buster
Daniel W
DW
Albert Einstein College of Medicine, Bronx, NY, USA.
Sharp
David J
DJ
D000818:Animals; D002460:Cell Line; D002875:Chromosomes / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure; D004330:Drosophila / Q000196:embryology / Q000502:physiology*; D018386:Kinetochores / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D008870:Microtubules / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D008941:Spindle Apparatus / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure