Analysis of microtubule-mediated intracellular viral transport.
Host microtubules and motor proteins are crucial to the intracellular transport of a number of viruses. Disruption of microtubules or suppression of motor functions can remarkably inhibit the movement of viruses in host cells. It is now known that incoming viruses use motor proteins to travel along microtubules from the plasma membrane to the nuclear or perinuclear replication site, whereas progeny viruses depend on microtubules and motors to move from the assembly site to the cell periphery. Here, we describe several major methods for analyzing microtubule-mediated intracellular viral transport, using adenovirus as an example.
Liu
Chunyong
C
Nankai University, Tianjin, China.
Liu
Min
M
Zhou
Jun
J
D000256:Adenoviridae / Q000737:chemistry / Q000502:physiology*; D001692:Biological Transport; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology / Q000821:virology; D002478:Cells, Cultured; D000072159:Dynactin Complex; D006801:Humans; D042541:Intracellular Space / Q000502:physiology* / Q000821:virology*; D008856:Microscopy, Fluorescence / Q000379:methods; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis; D008870:Microtubules / Q000737:chemistry* / Q000502:physiology* / Q000821:virology; D020409:Molecular Motor Proteins / Q000737:chemistry / Q000502:physiology; D014966:Xanthenes / Q000737:chemistry
D000072159:Dynactin Complex; D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; D014966:Xanthenes; C034657:Texas red