title: Roles of the genetic variation of alcohol-metabolizing enzymes on biomarkers in trauma patients with excessive alcohol intake at emergency department.
abstract: BACKGROUND
  Alcohol abuse has been implicated as an important factor for accidents. We evaluated the roles of different genetic combinations of the ADH2 and ALDH2 genotypes on biomarkers in trauma patients with excessive alcohol intake at our emergency department.
  
  
  METHODS
  Blood samples were obtained from 80 patients and 88 age-matched controls. The biomarkers, including AST, ALT, GGT, and MDA, were assayed. The polymerase chain reaction-restriction fragment length polymorphism method was used to determine the genetic polymorphisms of ADH2 and ALDH2.
  
  
  RESULTS
  There were significant differences in the levels of AST, ALT, GGT, MDA, and AST/ALT ratios between the 2 groups. In addition, MDA values and AST/ALT ratios were significantly higher in the patients with normal activity of ADH2 than the patients with low activity of ADH2. Meanwhile, regarding ALDH2 genotypes, there were significantly higher ratios of AST/ALT in the patients with low activity of ALDH2. The highest AST/ALT ratios and MDA values were in the patients with ADH2 (*2/*2) and ALDH2 (*1/*2 and *2/*2).
  
  
  CONCLUSIONS
  In conclusion, our results indicated that alcohol-induced liver damage or oxidative stress might be influenced by the genetic variation of ADH2 or ALDH2. Therefore, the combinations of different ADH2 and ALDH2 genotypes may be influential markers for susceptibility to alcohol-induced liver damage.
authors: Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors: Yang-Ming
authors: YM
authors: Tseng
authors: Yi-Ru
authors: YR
authors: Jin
authors: Ing-Jun
authors: IJ
authors: Chen
authors: Fong-Dee
authors: FD
authors: Huang
authors: Szu-Hsien
authors: SH
authors: Wu
authors: Hsu
authors: H
authors: Ma
authors: Sheng-Yi
authors: SY
authors: Chen
authors: Li-Yu
authors: LY
authors: Tsai
authors: Shih-Meng
authors: SM
authors: Tsai
authors: Jang-Hwa
authors: JH
authors: Lee
mesh_terms: D000328:Adult; D000368:Aged; D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000435:Alcoholic Intoxication / Q000201:enzymology*; D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D004636:Emergency Service, Hospital; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008875:Middle Aged; D014947:Wounds and Injuries / Q000201:enzymology*
chemical_list: D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase