Quantification of superoxide radical in the brain of rats with experimentally induced obstructive jaundice.
The study aimed to directly measure in vivo superoxide radical (O*2) a direct indicator of oxidative stress, in the brain of rats with experimentally induced obstructive jaundice by employing a new quantitative ultrasensitive fluorescent assay requiring minimum sample. O*2 anion is specific for dihydroethidine (DHE) and upon reaction gives a characteristic product, namely 2-OH-ethidium. Ten male rats underwent laparotomy and were divided into two groups: I, sham operated and II bile duct ligation. Ten days later, following injection with DHE (a O*2 trap), all animals were killed and samples from cerebral cortex, midbrain and cerebellum were removed for analysis. It was shown that compared to group I, in group II the O*2 was increased by 67% in the cerebral cortex and by 37% in the midbrain as a consequence of experimental obstructive jaundice, while its levels were unaffected in the cerebellum. The data in this experimental obstructive jaundice model imply a region-specific increase of O*2 formation rate, being higher in cerebral cortex, less so in the midbrain and not at all in cerebellum.
Konstantinou
Dimitris
D
Department of Neurosurgery, School of Medicine, University of Patras, Rion, Greece.
Mavrakis
Adamantios
A
Grintzalis
Konstantinos
K
Papapostolou
Ioannis
I
Assimakopoulos
Stelios F
SF
Chroni
Elisabeth
E
Georgiou
Christos
C
D000818:Animals; D001652:Bile Ducts / Q000601:surgery; D001921:Brain / Q000378:metabolism*; D003997:Dicarbethoxydihydrocollidine / Q000031:analogs & derivatives / Q000378:metabolism; D041781:Jaundice, Obstructive / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism*
C514241:dihydroethidine; D013481:Superoxides; D003997:Dicarbethoxydihydrocollidine