Synthetic lethal interactions identify phenotypic "interologs" of the spindle assembly checkpoint components.
Here, we report genetic interactions with mdf-1(gk2)/MAD1 in Caenorhabditis elegans. Nine are evolutionarily conserved or phenotypic "interologs" and two are novel enhancers, hcp-1 and bub-3. We show that HCP-1 and HCP-2, the two CENP-F-related proteins, recently implicated in the spindle assembly checkpoint (SAC) function, do not have identical functions, since hcp-1(RNAi), but not hcp-2(RNAi), enhances the lethality of the SAC mutants.
Tarailo
Maja
M
Department of Medical Genetics, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
Tarailo
Sanja
S
Rose
Ann M
AM
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D053263:Gene Regulatory Networks; D009687:Nuclear Proteins; D010641:Phenotype; D008941:Spindle Apparatus / Q000235:genetics*
D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; C400273:HCP-2 protein, C elegans; D009687:Nuclear Proteins; C400272:hcp-1 protein, C elegans