[Study on cryopreservation of Pueraria lobata germplasm by vitrification].
The experiment of the cryopreservation technique on Pueraria lobata showed that: At first, the aseptic explants were treated at 4 degrees C for 5 days. Then the stems with buds were cut and precultured at 4 degrees C for 1 day in 5% DMSO + 5% sucrose media. They were dehydrated with 60% and 100% PVS2 (30% glycerol + 15% glycol + 15% dimethyl sulfoxide + 0.4 mol/L sucrose) at 0 degrees C for 30 minutes respectively. At last the stems were immersed immediately into liquid nitrogen directly and conserved for 24 hours. After rapidly thawing in a water bath at 40 degrees C for 90 seconds, the stems were washed two times with MS media supplemented with 1.2 mol/L sucrose and 10 minutes each time, then transferred on the MS media supplemented with BA 2 mg/L and NAA 1 mg/l, in dark for 7 days and then in light. The survival rate was up to 57-58% . The regenerated plant of Pueraria lobata were the same as the normal in morphology.
Hong
Sen-rong
SR
Life Sciences Department, Shangrao Normal College, Shangrao 334001, China. hongsenrong@163.com
Yin
Ming-hua
MH
D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D046508:Culture Techniques / Q000379:methods; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D018547:Plant Stems / Q000187:drug effects / Q000254:growth & development; D010946:Plants, Medicinal / Q000187:drug effects / Q000254:growth & development*; D029908:Pueraria / Q000187:drug effects / Q000254:growth & development*; D013997:Time Factors
D003451:Cryoprotective Agents; D003470:Culture Media; D004121:Dimethyl Sulfoxide