Human parechovirus type 1, 3, 4, 5, and 6 detection in picornavirus cultures.
Picornavirus cultures that could not be typed in neutralization assays were analyzed by VP1 reverse transcription-PCR (RT-PCR) and a virus discovery tool (VIDISCA). Human parechoviruses (HPeVs) were frequently identified, among which were the uncommon isolates HPeV-4, HPeV-5, and HPeV-6. The HPeV-5 isolate could be amplified only by VIDISCA and not by VP1 RT-PCR.
de Vries
Michel
M
Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam, Academic Medical Center of University of Amsterdam, Amsterdam, The Netherlands.
Pyrc
Krzysztof
K
Berkhout
Ron
R
Vermeulen-Oost
Wilma
W
Dijkman
Ronald
R
Jebbink
Maarten F
MF
Bruisten
Sylvia
S
Berkhout
Ben
B
van der Hoek
Lia
L
D000595:Amino Acid Sequence; D006801:Humans; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D030061:Parechovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010849:Picornaviridae / Q000254:growth & development*; D010850:Picornaviridae Infections / Q000175:diagnosis / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D014776:Virus Cultivation
D014764:Viral Proteins