title: Assignment of the absolute configuration of P-chiral 5' mRNA cap analogues containing phosphorothioate moiety.
abstract: Enzymatic cleavage of the P-chiral diastereoisomers of the 5' mRNA cap analogue bearing phosphorothioate moiety in alfa position of 5',5'-triphosphate bridge (m(7)Gppp(S)G D1 and D2) was performed by human Decapping Scavenger (DcpS) enzyme. Analysis of the degradation products allowed to estimate the absolute configuration at the asymmetric phosphorus atoms in examined compounds via correlation with the R(P) and S(P) diastereoisomers of guanosine 5'-O-(1-thiodiphosphate) (GDPalphaS).
authors: Department of Biophysics, Institute of Experimental Physics, Warsaw University, Zwirki i Wigury 93, Warsaw 02-089, Poland.
authors: Joanna
authors: J
authors: Kowalska
authors: Magdalena
authors: M
authors: Lewdorowicz
authors: Joanna
authors: J
authors: Zuberek
authors: Elzbieta
authors: E
authors: Bojarska
authors: Janusz
authors: J
authors: Stepinski
authors: Ryszard
authors: R
authors: Stolarski
authors: Edward
authors: E
authors: Darzynkiewicz
authors: Jacek
authors: J
authors: Jemielity
mesh_terms: D004722:Endoribonucleases / Q000737:chemistry*; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives / Q000032:analysis; D006801:Humans; D006868:Hydrolysis; D009690:Nucleic Acid Conformation; D012314:RNA Cap Analogs / Q000737:chemistry*; D012315:RNA Caps / Q000737:chemistry*; D013873:Thionucleotides / Q000032:analysis
chemical_list: D012314:RNA Cap Analogs; D012315:RNA Caps; D013873:Thionucleotides; C527041:m(7)GpppsG nucleotide; D006153:Guanosine Diphosphate; C020339:guanosine 5'-O-(1-thiodiphosphate); D004722:Endoribonucleases; C485332:DcpS protein, human