[Porphyromonas gingivalis vesicle induced cellular inflammatory responses of gingival epithelial cells in vitro].
OBJECTIVE
To establish the model of cellular inflammatory responses of gingival epithelial cells in vitro induced by Porphyromonas gingivalis vesicle and to probe into the pathogenesis of Porphyromonas gingivalis in periodontitis.


METHODS
The effect of Porphyromonas gingivalis vesicle on prostaglandin E(2) (PGE(2)) production of gingival epithelial cells was detected by ELISA and the effects of Porphyromonas gingivalis vesicle on cyclooxygenase-2 (COX-2), interleukin (IL)-6 and IL-8 mRNA expression in gingival epithelial cells were determined by Real-time reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
Porphyromonas gingivalis vesicle dose-dependently induced PGE(2) production and up-regulated COX-2, IL-6 and IL-8 mRNA expression in gingival epithelial cells significantly.


CONCLUSIONS
Cellular inflammatory responses of gingival epithelial cells induced by Porphyromonas gingivalis vesicle may contribute to the initiation and progression of periodontitis.
Kou
Yu-Rong
YR
Department of Oral Medicine, School of Stomatology, China Medical University, Shenyang 110002, China.
Pan
Ya-Ping
YP
D001422:Bacterial Adhesion; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000276:immunology / Q000378:metabolism; D015232:Dinoprostone / Q000276:immunology / Q000378:metabolism; D004847:Epithelial Cells / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D005881:Gingiva / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D015850:Interleukin-6 / Q000276:immunology / Q000378:metabolism; D016209:Interleukin-8 / Q000276:immunology / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000276:immunology* / Q000472:pathogenicity
C508606:CXCL8 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D015232:Dinoprostone