[Expression, subcellular localization and nuclear translocation of transcription factor up stream stimulatory factor-1 in odontoblasts].
OBJECTIVE
To examine the expression and subcellular localization of transcription factor USF1 in odontoblasts and investigate whether nuclear translocation occurs under stimuli.


METHODS
Odontoblasts MDPC-23 were cultured on coverslips and divided into 2 groups. Group 1 received no stimuli, and group 2 was stimulated by nicotine with various concentrations respectively for 1h. Then the mountings of odontoblasts were prepared and immunocytochemical staining was performed with specific USF1 antibody via SABC method. Hela cells were used as positive control.


RESULTS
The staining was positive in the cytoplasm of odontoblasts in group 1, but in the nuclei of Hela cells and in 100 mg/L nicotine-stimulated odontoblasts in group 2.


CONCLUSIONS
There exists USF1 protein in odontoblasts, which locates in the cytoplasm and could translocate into nuclei under the stimulation of nicotine.
Wu
Li-An
LA
Department of Pediatric Dentistry, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Wen
Ling-Ying
LY
Yang
Fu-Sheng
FS
Wang
Xiao-Jing
XJ
Fang
Jun
J
D002478:Cells, Cultured; D006367:HeLa Cells; D006801:Humans; D009538:Nicotine / Q000494:pharmacology; D009804:Odontoblasts / Q000187:drug effects / Q000378:metabolism*; D021382:Protein Sorting Signals; D021381:Protein Transport / Q000187:drug effects; D051720:Upstream Stimulatory Factors / Q000378:metabolism*
D021382:Protein Sorting Signals; C497181:USF1 protein, human; D051720:Upstream Stimulatory Factors; D009538:Nicotine