title: Bornyl (3,4,5-trihydroxy)-cinnamate--an optimized human neutrophil elastase inhibitor designed by free energy calculations.
abstract: Human neutrophil elastase (HNE), a serine protease, is involved in the regulation of inflammatory processes and controlled by endogenous proteinase inhibitors. Abnormally high levels of HNE can cause degradation of healthy tissues contributing to inflammatory diseases such as rheumatoid arthritis, and also psoriasis and delayed wound healing. In continuation of our research on HNE inhibitors we have used the recently developed binding mode model for a group of cinnamic acid derivative elastase inhibitors and created bornyl (3,4,5-trihydroxy)-cinnamate. This ligand exhibited improved binding affinity predicted by means of free energy calculations. An organic synthesis scheme for the ligand was developed and its inhibitory activity was tested toward the isolated enzyme. Its IC(50) value was found to be three times lower than that of similar compounds, which is in line with the computational result showing the high potential of free energy calculations as a tool in drug development.
authors: Institut für Physikalische Chemie, Albert-Ludwigs-Universität Freiburg, Albertstr. 23a, 79104 Freiburg, Germany.
authors: Thomas
authors: T
authors: Steinbrecher
authors: Andrea
authors: A
authors: Hrenn
authors: Korinna L
authors: KL
authors: Dormann
authors: Irmgard
authors: I
authors: Merfort
authors: Andreas
authors: A
authors: Labahn
mesh_terms: D001892:Camphanes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D002934:Cinnamates / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D015394:Molecular Structure; D053491:Proteinase Inhibitory Proteins, Secretory / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D001892:Camphanes; D002934:Cinnamates; D053491:Proteinase Inhibitory Proteins, Secretory; C528614:bornyl (3,4,5-trihydroxy)-cinnamate; C029010:cinnamic acid