title: Exploration of associations between phospholipase A2 gene family polymorphisms and AIDS progression using the SNPlex method.
abstract: Members of the secreted phospholipase A2 (PLA2) protein family can inhibit HIV-1 virus replication in vitro. To evaluate the impact of PLA2 gene polymorphisms on AIDS disease development, we studied 12 family members using SNPlextrade mark technology that permitted simultaneous typing of 70 tagging Single Nucleotide Polymorphisms (tagSNPs). The study utilized HIV-1 seropositive donors with slow progressor (n=168) or rapid progressor (n=54) status, plus 355 control subjects. All donors were Caucasian (total 577 individuals). Genetic associations yielded mainly 0.01<p<0.05, but lower p-values were obtained for four tagSNPs and seven haplotype alleles. These stronger associations corresponded to both secreted (PLA2G2A, PLA2G2D and PLA2G3) and cytosolic (PLA2G4A and PLA2G6) PLA2 genes, including three (PLA2G2A, PLA2G2D and PLA2G4A) implicated in the pathogenesis of other diseases. Our results suggest that the PLA2 gene family may represent genes of interest for a larger study targeting all the known tagSNPs in the PLA2 genes. The data presented in this study will have to be confirmed in other AIDS cohorts and will also be useful for studies undertaken on the PLA2 gene family in other disease cohorts.
authors: CEA/Institut de Génomique, Centre National de Génotypage, 2 rue Gaston Crémieux, 91057 Evry, France.
authors: Sophie
authors: S
authors: Limou
authors: Cédric
authors: C
authors: Coulonges
authors: Mario
authors: M
authors: Foglio
authors: Simon
authors: S
authors: Heath
authors: Gora
authors: G
authors: Diop
authors: Sigrid
authors: S
authors: Leclerc
authors: Thomas
authors: T
authors: Hirtzig
authors: Jean-Louis
authors: JL
authors: Spadoni
authors: Amu
authors: A
authors: Therwath
authors: Gérard
authors: G
authors: Lambeau
authors: Ivo
authors: I
authors: Gut
authors: Jean-François
authors: JF
authors: Zagury
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics*; D000483:Alleles; D015331:Cohort Studies; D018450:Disease Progression; D005602:France; D020022:Genetic Predisposition to Disease; D023281:Genomics / Q000379:methods; D006239:Haplotypes; D006801:Humans; D054467:Phospholipases A2 / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D044465:White People
chemical_list: D054467:Phospholipases A2