title: Strain-specific detection of two Aureobasidium pullulans strains, fungal biocontrol agents of fire blight by new, developed multiplex-PCR.
abstract: OBJECTIVE
  The aim of this study was to develop a specific and sensitive identification method for two Aureobasidium pullulans biocontrol strains, CF10 and CF40, based on a sequence-characterized amplified region (SCAR) derived from RAPD - and multiplex-RAPD PCR analysis.
  
  
  RESULTS
  The random amplified polymorphic DNA (RAPD) and multiplex RAPD-PCR techniques were used for a preliminary screening of A. pullulans genetic variability among 200 isolates. This approach allowed the selection of ten fragments present solely in strains CF10 and CF40. The RAPD fragments were cloned, sequenced and used to design two SCAR primers. Two primer pairs obtained from SCH3RAPD fragment of CF 40 and 6RAPD of CF10 were highly specific and sensitive.
  
  
  CONCLUSIONS
  In this study, we developed strain-specific multiplex-PCR based on sequence-characterized amplified region (SCAR) markers to simultaneously detect both strains in a single PCR.
  
  
  CONCLUSIONS
  This new multiplex-PCR provides a valuable tool for specific and sensitive identification of CF10 and CF40, and could be used in studies on the efficacy and persistence of introduced strains of A. pullulans for fire blight control.
authors: Institute for Apiculture, Austrian Agency for Health and Food Safety (AGES), Vienna, Austria. igor.loncaric@ages.at
authors: I
authors: I
authors: Loncaric
authors: C
authors: C
authors: Donat
authors: B
authors: B
authors: Antlinger
authors: J T
authors: JT
authors: Oberlerchner
authors: B
authors: B
authors: Heissenberger
authors: R
authors: R
authors: Moosbeckhofer
mesh_terms: D000383:Agriculture; D001203:Ascomycota / Q000235:genetics / Q000302:isolation & purification*; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000032:analysis*; D005819:Genetic Markers; D007218:Industrial Microbiology; D010935:Plant Diseases / Q000382:microbiology; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D004271:DNA, Fungal; D005819:Genetic Markers