[Gene screening in five Chinese families with hereditary spastic paraplegia with thin corpus callosum].
OBJECTIVE
To screen all ten genes between D15S971 and D15S1012 in five Chinese families with hereditary spastic paraplegia with thin corpus callosum (HSP-TCC).


METHODS
DNA samples from 5 HSP-TCC families were screened for mutations in AK128197, MGC14798, HH114, MEIS2, MGC35118, SPRED1, AK128458, FLJ38426, RASGRP1 and AK093014 on chromosome 15q13-15 between microsatellites D15S971 and D15S1012 by polymerase chain reaction, direct sequencing and cosegreagation analysis.


RESULTS
No disease-causing mutations were found in the 10 genes, but 13 polymorphisms were identified in which two were novel.


CONCLUSIONS
This study did not support the ten genes between D15S971 and D15S1012 were the disease-causing genes of the 5 HSP-TCC families.
Zhao
Guo-hua
GH
Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008 PR China.
Guo
Peng
P
Ren
Zhi-jun
ZJ
Liu
Xiao-min
XM
Shen
Lu
L
Xia
Kun
K
Tang
Bei-sha
BS
D000328:Adult; D044466:Asian People / Q000235:genetics; D002884:Chromosomes, Human, Pair 15; D003337:Corpus Callosum / Q000473:pathology*; D005260:Female; D005808:Genes, Recessive; D006801:Humans; D008297:Male; D020336:Paraparesis, Spastic / Q000235:genetics; D015419:Spastic Paraplegia, Hereditary / Q000150:complications / Q000235:genetics*