Assessment by electron-microscopy of recombinant Vibrio cholerae and Salmonella vaccine strains expressing enterotoxigenic Escherichia coli-specific surface antigens.
Diarrhoea caused by enterotoxigenic Escherichia coli (ETEC) requires adhesion of microorganisms to enterocytes. Hence, a promising approach to immunoprophylaxis is to elicit antibodies against colonisation factor antigens (CFAs). Genes encoding the most prevalent ETEC-specific surface antigens were cloned into Vibrio cholerae and Salmonella vaccine strains. Expression of surface antigens was assessed by electron-microscopy. Whereas negative staining was effective in revealing CFA/I and CS3, but not CS6, immunolabelling allowed identification of all surface antigens examined. The V. cholerae vaccine strain CVD103 did not express ETEC-specific colonisation factors, whereas CVD103-HgR expressed CS3 only. However, expression of both CFA/I and CS3 was demonstrated in Salmonella Ty21a.
Ziethlow
V
V
Division of Veterinary Anatomy, Vetsuisse Faculty University of Berne, Berne, Switzerland.
Favre
D
D
Viret
J-F
JF
Frey
J
J
Stoffel
M H
MH
D000942:Antigens, Bacterial / Q000032:analysis* / Q000235:genetics; D000954:Antigens, Surface / Q000032:analysis* / Q000235:genetics; D001428:Bacterial Vaccines / Q000737:chemistry* / Q000276:immunology; D022121:Cholera Vaccines / Q000737:chemistry* / Q000276:immunology; D003001:Cloning, Molecular; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000032:analysis* / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D012475:Salmonella / Q000737:chemistry / Q000276:immunology; D022562:Salmonella Vaccines / Q000737:chemistry* / Q000276:immunology; D014734:Vibrio cholerae / Q000737:chemistry / Q000276:immunology
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001428:Bacterial Vaccines; D022121:Cholera Vaccines; D029968:Escherichia coli Proteins; D022562:Salmonella Vaccines