[Differences of signaling pathway of lung injury between C57BL/6 and C3H/He mice exposed to fine particle].
OBJECTIVE
To study the differences of signaling pathway of lung injury between C57BL/6 and C3H/He mice exposed to fine particle.


METHODS
By intratracheally instillation, two strains of mice (C57BL/6 mice and C3 H/He) were exposed to fine particle once time per day, for continuously two days. Twenty-four hours after last time exposure, the mice were killed and the lungs were taken out immediately, and put into the liquid nitrogen. By using Affymetrix430 2.0 genechip, differences of the signaling pathways between C57BL/6 and C3H/He mice were found.


RESULTS
It was indicated that the ratios of (C57BL/6)/(C3H/He) of Igh-6, Mmp2, Timp1, Col1a1, Col1a2, C4 and Hc expression in unexposed mice,and in exposed mice were 0.00, -2.40, 0.00, -4.42, -4.92, 6.65 and -1.93, and -2.83, 2.15, -2.18, 2.40, 2.86, 4.23 and 2.18, respectively. And by further analysis, three different signaling pathways were discovered between C57BL/6 and C3H/He mice, including inflammatory response pathway, matrix metalloproteinases pathway and classical complement activation.


CONCLUSIONS
The results showed that three signaling pathways were associated with inflammation. It was suggest that the lung inflammation in C57BL/6 mice could be more severe than that in C3H/He mice. The possible causation may be the different genetic background between C57BL/6 and C3H/He mice.
Cao
Qiang
Q
School of Public Health, Fudan University, Shanghai 200032, China.
Zhang
Shu
S
Jiang
Zhihai
Z
Li
Qi
Q
D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D004781:Environmental Exposure / Q000009:adverse effects; D005786:Gene Expression Regulation; D055370:Lung Injury / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D052638:Particulate Matter / Q000633:toxicity*; D011897:Random Allocation; D015398:Signal Transduction / Q000235:genetics*; D013045:Species Specificity
D000393:Air Pollutants; D052638:Particulate Matter