Phylogenetic analysis of Cystoisospora species at the rRNA ITS1 locus and development of a PCR-RFLP assay.
The ITS1 sequences for C. suis, C. belli, C. rivolta, C. felis, and C. ohioensis-like oocysts were determined and a diagnostic PCR-RFLP assay specific for Cystosisopora species was developed. Phylogenetic analysis of ITS1 sequences of Cystosisopora species along with ITS1 sequences for Toxoplasma, Neospora, Sarcocystis and Eimeria spp. using distance, minimum evolution and parsimony-based methods confirmed previous studies, which suggested that the genus Cystoisospora does not belong to the family Eimeriidae, but should be classified together with the cyst-forming coccidia in the family Sarcocystidae.
Samarasinghe
Bimba
B
Division of Health Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia.
Johnson
Johanna
J
Ryan
Una
U
D000818:Animals; D001483:Base Sequence; D002415:Cats; D003048:Coccidiosis / Q000175:diagnosis / Q000469:parasitology*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry*; D004285:Dogs; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D021745:Sarcocystidae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D013552:Swine
D021903:DNA, Ribosomal Spacer