title: Mitochondrial dysfunction is a possible cause of accelerated senescence of mesothelial cells exposed to high glucose.
abstract: High glucose has been found to accelerate cell senescence in vitro. The exact mechanism of this effect is, however, still poorly understood. In this paper we show that human peritoneal mesothelial cells (HPMCs) propagated under high (30mM) glucose were characterized by higher density of DNA double-strand breaks than cells exposed to standard (5mM) glucose concentration. Under both low and high glucose conditions, the vast majority of DNA damage localized to non-telomeric regions of the genome. Moreover, exposure to high glucose resulted in increased accumulation of lipofuscin, increased production of superoxides and peroxides as well as reduced mitochondrial membrane potential and increased mitochondrial mass. Treatment of cells with the free radical scavenger PBN partially rescued the premature senescence caused by high glucose. Together, these results indicate that high glucose may accelerate senescence of HPMCs by impairing mitochondrial function, resulting in overproduction of reactive oxygen species and extensive DNA damage.
authors: Department of Pathophysiology, University of Medical Sciences, Swiecickiego 6, 60781 Poznań, Poland.
authors: Krzysztof
authors: K
authors: Ksiazek
authors: João F
authors: JF
authors: Passos
authors: Sharon
authors: S
authors: Olijslagers
authors: Thomas
authors: T
authors: von Zglinicki
mesh_terms: D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology*; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure*; D005947:Glucose / Q000008:administration & dosage*; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology
chemical_list: D005947:Glucose