Transcriptional response of murine macrophages upon infection with opsonized Paracoccidioides brasiliensis yeast cells.
Paracoccidioides brasiliensis is the etiologic agent of the Paracoccidioidomycosis the most common systemic mycosis in Latin America. Little is known about the regulation of genes involved in the innate immune host response to P. brasiliensis. We therefore examined the kinetic profile of gene expression of peritoneal macrophage infected with P. brasiliensis. Total RNA from macrophages at 6, 24 and 48h was extracted, hybridized onto nylon membranes and analyzed. An increase in the transcription of a number of pro-inflammatory molecules encoding membrane proteins, metalloproteases, involved in adhesion and phagocytosis, are described. We observed also the differential expression of genes whose products may cause apoptotic events induced at 24h. In addition, considering the simultaneous analyses of differential gene expression for the pathogen reported before by our group, at six hours post infection, we propose a model at molecular level for the P. brasiliensis-macrophage early interaction. In this regard, P. brasiliensis regulates genes specially related to stress and macrophages, at the same time point, up-regulate genes related to inflammation and phagocytosis, probably as an effort to counteract infection by the fungus.
Silva
Simoneide S
SS
Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF 70910-900, Brazil.
Tavares
Aldo Henrique F P
AH
Passos-Silva
Danielle G
DG
Fachin
Ana L
AL
Teixeira
Santuza M R
SM
Soares
Célia M A
CM
Carvalho
Maria José A
MJ
Bocca
Anamélia L
AL
Silva-Pereira
Ildinete
I
Passos
Geraldo A S
GA
Felipe
Maria Sueli Soares
MS
D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D007249:Inflammation / Q000235:genetics / Q000276:immunology; D018836:Inflammation Mediators / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000382:microbiology*; D051379:Mice; D008954:Models, Biological; D010228:Paracoccidioides / Q000276:immunology*; D010587:Phagocytosis; D013997:Time Factors; D015854:Up-Regulation
D018836:Inflammation Mediators