Length of pressure-controlled reperfusion is critical for reducing ischaemia-reperfusion injury in an isolated rabbit lung model.
BACKGROUND
Ischaemia-reperfusion injury is still a major problem after lung transplantation. Several reports describe the benefits of controlled graft reperfusion. In this study the role of length of the initial pressure-controlled reperfusion (PCR) was evaluated in a model of isolated, buffer-perfused rabbit lungs.


METHODS
Heart-lung blocks of 25 New Zealand white rabbits were used. After measurement of baseline values (haemodynamics and gas exchange) the lungs were exposed to 120 minutes of hypoxic warm ischaemia followed by repeated measurements during reperfusion. Group A was immediately reperfused using a flow of 100 ml/min whereas groups B, C and D were initially reperfused with a maximum pressure of 5 mmHg for 5, 15 or 30 minutes, respectively. The control group had no period of ischaemia or PCR.


RESULTS
Uncontrolled reperfusion (group A) caused a significant pulmonary injury with increased pulmonary artery pressures (PAP) and pulmonary vascular resistance and a decrease in oxygen partial pressure (PO2), tidal volume and in lung compliance. All groups with PCR had a significantly higher PO2 for 5 to 90 min after start of reperfusion. At 120 min there was also a significant difference between group B (264 +/- 91 mmHg) compared to groups C and D (436 +/- 87 mmHg; 562 +/- 20 mmHg, p < 0.01). All PCR groups showed a significant decrease of PAP compared to group A.


CONCLUSIONS
Uncontrolled reperfusion results in a severe lung injury with rapid oedema formation. PCR preserves pulmonary haemodynamics and gas exchange after ischaemia and might allows for recovery of the impaired endothelial function. 30 minutes of PCR provide superior results compared to 5 or 15 minutes of PCR.
Guth
Stefan
S
Department of Cardiothoracic and Vascular Surgery, Johannes Gutenberg - University, Langenbeckstr, 1, 55131 Mainz, Germany. guth@diako-online.de
Prüfer
Diethard
D
Kramm
Thorsten
T
Mayer
Eckhard
E
D000818:Animals; D004195:Disease Models, Animal; D008168:Lung / Q000378:metabolism / Q000503:physiopathology*; D008170:Lung Compliance; D055370:Lung Injury / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D009929:Organ Size; D010100:Oxygen / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D011312:Pressure; D011651:Pulmonary Artery / Q000503:physiopathology; D011817:Rabbits; D015424:Reperfusion / Q000379:methods*; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology* / Q000517:prevention & control*; D014655:Vascular Resistance; D052096:Warm Ischemia / Q000379:methods*
D009195:Peroxidase; D010100:Oxygen