Crystallization and preliminary crystallographic studies of an active-site mutant hydantoin racemase from Sinorhizobium meliloti CECT4114.
A recombinant active-site mutant of hydantoin racemase (C76A) from Sinorhizobium meliloti CECT 4114 (SmeHyuA) has been crystallized in the presence and absence of the substrate D,L-5-isopropyl hydantoin. Crystals of the SmeHyuA mutant suitable for data collection and structure determination were grown using the counter-diffusion method. X-ray data were collected to resolutions of 2.17 and 1.85 A for the free and bound enzymes, respectively. Both crystals belong to space group R3 and contain two molecules of SmeHyuA per asymmetric unit. The crystals of the free and complexed SmeHyuA have unit-cell parameters a = b = 85.43, c = 152.37 A and a = b = 85.69, c = 154.38 A, crystal volumes per protein weight (V(M)) of 1.94 and 1.98 A3 Da(-1) and solvent contents of 36.7 and 37.9%, respectively.
Martínez-Rodríguez
Sergio
S
Department of Cellular and Molecular Interactions, Vrije Universiteit Brussel, 1050 Brussels, Belgium.
González-Ramírez
Luis Antonio
LA
Clemente-Jiménez
Josefa María
JM
Rodríguez-Vico
Felipe
F
Las Heras-Vázquez
Francisco Javier
FJ
Gavira
Jose Antonio
JA
García-Ruiz
Juan Ma
JM
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D001665:Binding Sites; D007202:Indicators and Reagents; D019998:Racemases and Epimerases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D016962:Sinorhizobium meliloti / Q000201:enzymology*
D001426:Bacterial Proteins; D007202:Indicators and Reagents; D011994:Recombinant Proteins; D019998:Racemases and Epimerases; C074773:hydantoin racemase