Emerging role of new transgenic mouse models in glioma research.
Our understanding of glioma biology has relied heavily on the use of cell lines and xenograft animal models. However, the recent development of transgenic mouse models offers a unique opportunity to examine the pathophysiology of these tumors in immunocompetent models in vivo. Transgenic models are highly informative for a number of reasons. First, the resulting tumors are genetically and histologically similar to human gliomas. Second, transgenic models allow the study of causality of genetic/pathway alterations reminiscent of human gliomas. Third, new therapies can be tested in established tumors to truly evaluate their potential efficacy. This review describes the available technologies involved in transgenic and knockout mouse modeling, including the generation of cell-type-specific genetic alterations. Finally, genetics are discussed with a focus on how transgenic murine gliomas recapitulate alterations found in human counterparts.
Sonabend
Adam M
AM
The University of Chicago Brain Tumor Center, The University of Chicago Section of Neurosurgery, Chicago, IL, USA. adam.sonabend@gmail.com
Ulasov
Ilya V
IV
Lesniak
Maciej S
MS
D000818:Animals; D001254:Astrocytoma / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D017024:Chemotherapy, Adjuvant; D004195:Disease Models, Animal; D005260:Female; D005910:Glioma / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D006801:Humans; D007150:Immunohistochemistry; D007167:Immunotherapy / Q000379:methods; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008967:Molecular Biology; D018714:Radiotherapy, Adjuvant; D012107:Research Design; D012680:Sensitivity and Specificity