[Study on the histopathology of cats inoculated with H5N1 subtype high pathogenic avian influenza virus originated from tigers].
In this study, the HPAIV A/Tiger/Harbin/01/2002 (H5N1) used was originated from tigers and propagated in SPF embryonated hen eggs. TCID5, of the virus was 10(-7.36)/0. 05mL on MDCK cell. The cats were inoculated through bronchus route and then, the cats of dead and control were collected for histopathological and immunohistochemistry examination. Meanwhile, the emulsion supernatant fluid of organs and the pharyngeal swab samples of the dead cats were collected for RT-PCR, survived cats and the control cats were tested for the presence of HI antibody by standard method. The results indicated that the damage of lungs from the dead cats were most obvious, the wide range of red consolidation focus emerged on the lobus pulmonis, the fused focus of infection caused injury of lungs. Histology under the microscope revealed diffuse alveolar damage, confluence phlegmasia pathology, infiltration of lymphomonocytes, sackful of infiltration of macrophages and manipulus protein-like effusion in the alveolar. By immunohistochemistry, the positively stained virus particles were found on the epithelial cells of bronchus and alveolus, and also in the endochylema of lymphomonocytes. The specific electophoretic band of 464bp amplified by RT-PCR from samples of pharyngeal swabs, lungs, kidneys, hearts and brains was as same as the theory value. HI antibody titers of the survived cat were 1:32.
Chang
Shuang
S
Institute of Military Veterinary, Academy of Military Medical Sciences of PLA, Changchun 130062, China.
Ding
Zhuang
Z
Yang
Song-Tao
ST
Gao
Yu-Wei
YW
Zou
Xiao-Huan
XH
Wang
Tie-Cheng
TC
Xia
Xian-Zhu
XZ
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002371:Cat Diseases / Q000473:pathology*; D002415:Cats; D006385:Hemagglutination Inhibition Tests; D007150:Immunohistochemistry; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology / Q000472:pathogenicity*; D009976:Orthomyxoviridae Infections / Q000473:pathology* / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046010:Tigers / Q000821:virology*
D000914:Antibodies, Viral