title: Virulence and pathogenicity patterns of Trypanosoma brucei gambiense field isolates in experimentally infected mouse: differences in host immune response modulation by secretome and proteomics.
abstract: Human African trypanosomiasis is characterised by an important clinical diversity. Although Trypanosoma brucei gambiense field stocks isolated from patients in the same focus did not exhibit apparent genetic variability, they showed marked differences in terms of virulence (capacity to multiply inside a host) and pathogenicity (ability of producing mortality) in experimental murine infections. Two strains exhibiting opposite pathogenic and virulence properties in mouse were further investigated through their host-parasite interactions. In vitro, parasite bloodstream forms or soluble factors (or secretome) from both strains induced macrophage arginase as a function of their virulence. Arginase expression, a hallmark of macrophage alternative activation pathway, favours trypanosome bloodstream forms development. Moreover, a comparative proteomic study of the trypanosome stocks' secretomes evidenced both a differential expression of common molecules and the existence of stock specific molecules. This highlighted the potential involvement of the differential expression of the same genome in the diverse infectious properties of trypanosomes.
authors: CIRAD, UMR 17 Trypanosomes, TA A-17/G, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France. philippe.holzmuller@cirad.fr
authors: Philippe
authors: P
authors: Holzmuller
authors: David G
authors: DG
authors: Biron
authors: Pierrette
authors: P
authors: Courtois
authors: Mathurin
authors: M
authors: Koffi
authors: Rachel
authors: R
authors: Bras-Gonçalves
authors: Sylvie
authors: S
authors: Daulouède
authors: Philippe
authors: P
authors: Solano
authors: Gérard
authors: G
authors: Cuny
authors: Philippe
authors: P
authors: Vincendeau
authors: Vincent
authors: V
authors: Jamonneau
mesh_terms: D000818:Animals; D001119:Arginase / Q000096:biosynthesis; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006790:Host-Parasite Interactions; D006801:Humans; D008264:Macrophages / Q000201:enzymology / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D018512:Parasitemia; D020543:Proteome / Q000032:analysis; D015800:Protozoan Proteins / Q000096:biosynthesis; D016019:Survival Analysis; D014347:Trypanosoma brucei gambiense / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity*; D014353:Trypanosomiasis, African / Q000469:parasitology; D014774:Virulence
chemical_list: D020543:Proteome; D015800:Protozoan Proteins; D001119:Arginase