title: Identification and molecular evolution of cow CENP-A gene family.
abstract: The centromere protein A (CENP-A), a histone H3-like protein, provides an essential role for chromosomal segregation during mitosis and meiosis. In this study we identified ten new CENP-A-like genes (excluding the original CENP-A gene) in cow by searching its genome database, while all other examined mammals contained only a single copy of the CENP-A gene. Phylogenetic analysis shows that the expansion of these genes occurred before the divergence of cow and sheep but after the artiodactyls diverged from other mammals. Our analyses also indicate that multiple gene duplication and intron loss events have occurred during the formation of this gene family, and the high similarity in intron sequences between the new genes and the old one suggests very recent gene expansion events. Furthermore, evidence from the comparisons of the synonymous and nonsynonymous substitutions and the distributions of the mutation sites suggested that the CENP-A-L-1 gene, a potentially functional member of cow CENP-A gene family, evolved under positive selection.
authors: Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, Peoples Republic of China. liying02@mail.kiz.ac.cn
authors: Ying
authors: Y
authors: Li
authors: Jing-Fei
authors: JF
authors: Huang
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D033481:Chromosomes, Mammalian / Q000235:genetics; D019143:Evolution, Molecular; D005810:Multigene Family / Q000235:genetics*; D010802:Phylogeny
chemical_list: D001324:Autoantigens; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone