title: Development of amphiphilic gamma-PGA-nanoparticle based tumor vaccine: potential of the nanoparticulate cytosolic protein delivery carrier.
abstract: Nanoscopic therapeutic systems that incorporate biomacromolecules, such as protein and peptides, are emerging as the next generation of nanomedicine aimed at improving the therapeutic efficacy of biomacromolecular drugs. In this study, we report that poly(gamma-glutamic acid)-based nanoparticles (gamma-PGA NPs) are excellent protein delivery carriers for tumor vaccines that delivered antigenic proteins to antigen-presenting cells and elicited potent immune responses. Importantly, gamma-PGA NPs efficiently delivered entrapped antigenic proteins through cytosolic translocation from the endosomes, which is a key process of gamma-PGA NP-mediated anti-tumor immune responses. Our findings suggest that the gamma-PGA NP system is suitable for the intracellular delivery of protein-based drugs as well as tumor vaccines.
authors: Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors: Tomoaki
authors: T
authors: Yoshikawa
authors: Naoki
authors: N
authors: Okada
authors: Atsushi
authors: A
authors: Oda
authors: Kazuhiko
authors: K
authors: Matsuo
authors: Keisuke
authors: K
authors: Matsuo
authors: Yohei
authors: Y
authors: Mukai
authors: Yasuo
authors: Y
authors: Yoshioka
authors: Takami
authors: T
authors: Akagi
authors: Mitsuru
authors: M
authors: Akashi
authors: Shinsaku
authors: S
authors: Nakagawa
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology*; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology; D004337:Drug Carriers / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011099:Polyglutamic Acid / Q000737:chemistry*; D011506:Proteins / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology*
chemical_list: D019496:Cancer Vaccines; D004337:Drug Carriers; D011506:Proteins; D011099:Polyglutamic Acid