title: [Spontaneous response and mechanism to ischemia in diabetic mouse].
abstract: OBJECTIVE
  To investigate the spontaneous response and its mechanism of type 2 diabetes mouse to surgical induced hind limb ischemia.
  
  
  METHODS
  Forty mice were randomly divided into two groups, one was fed with normal chow, and another was fed with high fat diet. Twelve weeks later, mice were surgical induced to hind limb ischemia. Blood flow was monitored. Four weeks later, mice were sacrificed and muscle tissues were harvested for histological detection, Western Blot, ELISA and RT-PCR examination respectively.
  
  
  RESULTS
  Spontaneous restoration of blood flow was significantly slow down in type 2 diabetic mice with lower level of vascular density in ischemic muscles. VEGF signaling pathway was impaired although the VEGF levels were increased in diabetes.
  
  
  CONCLUSIONS
  The spontaneous response to ischemia was attenuated with an abnormal reaction of VEGF signaling pathway in the setting of type 2 diabetes mellitus.
authors: Department of Vascular Surgery, Peking Union Medical College Hospital, and Chinese Academy of Medical Sciences, Beijing 100730, China. yongj_93@hotmail.com
authors: Yong-jun
authors: YJ
authors: Li
authors: Qing
authors: Q
authors: Lin
authors: Chang-wei
authors: CW
authors: Liu
authors: Heng
authors: H
authors: Guan
authors: Xia-lin
authors: XL
authors: Liu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000503:physiopathology*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D006614:Hindlimb / Q000098:blood supply / Q000378:metabolism; D007511:Ischemia / Q000209:etiology / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D011897:Random Allocation; D040262:Receptors, Vascular Endothelial Growth Factor / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D042461:Vascular Endothelial Growth Factor A; D040262:Receptors, Vascular Endothelial Growth Factor