Global transcriptional profile of Mycobacterium tuberculosis during THP-1 human macrophage infection.
During lung infection, Mycobacterium tuberculosis resides in macrophages and subverts the bactericidal mechanisms of these professional phagocytes. Comprehension of this host-pathogen relationship is fundamental for the development of new therapies to cure and prevent tuberculosis. In this work, we analyzed the transcriptional profile of M. tuberculosis infecting human macrophage-like THP-1 cells in order to identify putative bacterial pathogenic factors that can be relevant for the intracellular survival of M. tuberculosis. We compared the gene expression profile of M. tuberculosis H37Rv after 4 h and 24 h of infection of human macrophage-like THP-1 cells with the gene expression profile of the strain growing exponentially in broth cultures. We found 585 genes expressed differentially by intracellular M. tuberculosis. An analysis of the gene expression profile of M. tuberculosis inside THP-1 cells suggests the perturbation of the cell envelope as a major intracellular stress inside THP-1 macrophages.
Fontán
Patricia
P
TB Center, The Public Health Research Institute, UMDNJ-New Jersey Medical School, 225 Warren Street, Newark, New Jersey 07103-3535, USA.
Aris
Virginie
V
Ghanny
Saleena
S
Soteropoulos
Patricia
P
Smith
Issar
I
D002460:Cell Line; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000254:growth & development*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors