title: Identification of a new quaternary association for legume lectins.
abstract: Lotus tetragonolobus lectin (LTA) is a fucose-specific legume lectin. Although several studies report a diverse combination of biological activities for LTA, little is known about the mechanisms involved in l-fucosyl oligosaccharide recognition. The crystal structure of LTA at 2.0A resolution reveals a different legume lectin tetramer. Its structure consists of a homotetramer composed of two back-to-back GS4-like dimers arranged in a new mode, resulting in a novel tetramer. The LTA N-linked carbohydrate at Asn4 and the unusual LTA dimer-dimer interaction are related to its particular mode of tetramerization. In addition, we used small angle X-ray scattering to investigate the quaternary structure of LTA in solution and to compare it to the crystalline structure. Although the crystal structure of LTA has revealed a conserved metal-binding site, its l-fucose-binding site presents some punctual differences. Our investigation of the new tetramer of LTA and its fucose-binding site is essential for further studies related to cross-linking between LTA and complex divalent l-fucosyl carbohydrates.
authors: Curso de Pós-graduação em Biofísica Molecular, Departamento de Física, Universidade Estadual Paulista Júlio de Mesquita Filho, R. Cristovão Colombo, 2265, Nazareth, São José do Rio Preto, São Paulo, Brazil.
authors: Frederico Bruno Mendes Batista
authors: FB
authors: Moreno
authors: Taianá Maia
authors: TM
authors: de Oliveira
authors: Daiana Evelin
authors: DE
authors: Martil
authors: Magno Massolino
authors: MM
authors: Viçoti
authors: Gustavo Arruda
authors: GA
authors: Bezerra
authors: José Ramon Beltran
authors: JR
authors: Abrego
authors: Benildo Sousa
authors: BS
authors: Cavada
authors: Walter
authors: W
authors: Filgueira de Azevedo
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D018360:Crystallography, X-Ray; D019281:Dimerization; D005643:Fucose / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*; D000070116:Lotus; D008969:Molecular Sequence Data; D037121:Plant Lectins / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D012542:Scattering, Radiation
chemical_list: D037102:Lectins; D037121:Plant Lectins; C027112:fucose-binding lectin; D005643:Fucose