Ischemic postconditioning inhibits apoptosis after renal ischemia/reperfusion injury in rat.
Ischemic postconditioning is a phenomenon that intermittent interruptions of blood flow in the early phase of reperfusion can protect organ from ischemia/reperfusion (I/R) injury. In the present study, we investigated whether the protective effect of ischemic postconditioning was associated with modulation of apoptosis after renal I/R injury. Rats were subjected to 45 min of renal ischemia, both with and without treatment with ischemic postconditioning. Serum urea nitrogen and creatinine levels, phosphorylation of Akt and ERK1/2 and apoptosis were compared after renal injury. Our data showed that ischemic postconditioning attenuated the renal dysfunction and cell apoptosis induced by I/R and increased phosphorylation of Akt and ERK1/2. The results indicated that ischemic postconditioning decreased apoptosis and improved renal function. This protective effect may be related with the levels of Akt and ERK1/2 activation. These findings may have major implications in the treatment of renal transplantation.
Chen
Hui
H
Department of Urology, Renmin Hospital of Wuhan University, Wuhan University, Wuhan, China.
Xing
Bianzhi
B
Liu
Xiuheng
X
Zhan
Bingyan
B
Zhou
Jiangqiao
J
Zhu
Hengcheng
H
Chen
Zhiyuan
Z
D000818:Animals; D017209:Apoptosis; D004195:Disease Models, Animal; D019194:Ischemic Preconditioning; D007668:Kidney / Q000098:blood supply* / Q000503:physiopathology*; D007674:Kidney Diseases / Q000503:physiopathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000503:physiopathology*