title: Identification of a fatty acyl-CoA synthetase gene, lcf2+, which affects viability after entry into the stationary phase in Schizosaccharomyces pombe.
abstract: The lcf1(+) gene, which encodes a long chain fatty acyl-CoA synthetase, is necessary for the maintenance of viability after entry into the stationary phase in Schizosaccharomyces pombe. In this study, we analyzed a paralogous gene, SPBP4H10.11c (named lcf2(+)), and we present evidence that the gene encodes a new fatty acyl-CoA synthetase. The enzyme preferentially recognized myristic acid as a substrate. A Deltalcf2 mutant showed increased viability after entry into the stationary phase in SD medium. A Deltalcf1Deltalcf2 double mutant showed a severe decrease in long-chain fatty acyl-CoA synthetase activity and a rapid loss of viability after entry into the stationary phase. These results suggest that fatty acid utilization and/or metabolism is important to determine viability in the stationary phase.
authors: Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Nagoya, Japan.
authors: Yasuyuki
authors: Y
authors: Fujita
authors: Satoka
authors: S
authors: Mita
authors: Hokuto
authors: H
authors: Ohtsuka
authors: Hirofumi
authors: H
authors: Aiba
mesh_terms: D000214:Acyl Coenzyme A / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D050296:Microbial Viability; D008969:Molecular Sequence Data; D009154:Mutation; D019814:Myristic Acid / Q000378:metabolism; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000502:physiology; D013379:Substrate Specificity
chemical_list: D000214:Acyl Coenzyme A; D019814:Myristic Acid