[Identifying group G beta-hemolytic Streptococci strains by 16S rDNA sequencing].
OBJECTIVE
To identify the DNA sequences of 26 beta-hemolytic group G streptococci strains isolated from the swabs of patients in a glomerulonephritis outbreak event.


METHODS
API20 Strep system and 16S rDNA sequencing were performed on all of the 26 isolated strains respectively. The dendrogram based on the analysis of the 16S rDNA sequences of four groups of G streptococci strains was constructed by the neighbor-joining method and boot-strap test.


RESULTS
The API20 Strep system failed to identify the strains with less than 62% of success rates. All of the 26 group G beta-hemolytic streptococci isolates were identified as Streptococcus anginosus by the 16S rDNA sequencing, with more than 97% of success rates. The dendrogram illustrated that the four groups of G streptococci strains were clustered at the same branch with Streptococcus anginosus.


CONCLUSIONS
The 16S rDNA sequencing has unique advantages over the phenotypic methods. It can provide detailed information of nucleotides and identify species of beta-hemolytic G group streptococci.
Deng
Xiao-ling
XL
Center for Disease Control and Prevention of Guangdong Province, Guangzhou 510300, China.
Ke
Bi-xia
BX
Ke
Chang-wen
CW
Wang
Hong-min
HM
Zhong
Hao-jie
HJ
Li
Ling-hui
LH
Luo
Hui-ming
HM
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013291:Streptococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S