Sustained hypoxia-induced proliferation of carotid body type I cells in rats.
Sustained hypoxia (SH) has been shown to cause profound morphological and cellular changes in carotid body (CB). However, results regarding whether SH causes CB type I cell proliferation are conflicting. By using bromodeoxyuridine, a uridine analog that is stably incorporated into cells undergoing DNA synthesis, we have found that SH causes the type I cell proliferation in the CB; the proliferation occurs mainly during the first 1-3 days of hypoxic exposure. Moreover, the new cells survive for at least 1 mo after the return to normoxia. Also, SH does not cause any cell death in CB as examined by the terminal deoxynucleotidyl transferase-mediated dUTP-X nick-end labeling assay. Taken together, our results suggest that SH stimulates CB type I cell proliferation, which may produce long-lasting changes in CB morphology and function.
Wang
Z-Y
ZY
Dept. of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA. wangz@svm.vetmed.wisc.edu
Olson
E B
EB
Bjorling
D E
DE
Mitchell
G S
GS
Bisgard
G E
GE
D000818:Animals; D017209:Apoptosis; D001973:Bromodeoxyuridine; D002344:Carotid Body / Q000473:pathology*; D049109:Cell Proliferation; D002470:Cell Survival; D004261:DNA Replication; D004195:Disease Models, Animal; D000860:Hypoxia / Q000473:pathology*; D008297:Male; D009336:Necrosis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D001973:Bromodeoxyuridine