[Detecting perfluorooctanoic acid by reversed phase high performance liquid chromatography with pre-column ultraviolet derivatization].
OBJECTIVE
To establish a reversed phase high performance liquid chromatographic method with pre-column ultraviolet derivatization for the determination of perfluorooctanoic acids (PFOA) in sewage and urine.


METHODS
PFOA in sewage and urine formed an ion-pair with tetrabutylammonium (TBA) and were extracted into MTBE. The PFOA then reacted with omega-bromoacetophenone (omega-BAP) in acetonitrile and produced materials quantifiable by HPLC with UV detector.


RESULTS
The detection limit of the proposed method was 7.0 microg/L with a relative standard deviation of less than 10%. The recoveries for real samples ranged from 85.3% to 116%.


CONCLUSIONS
The proposed method is suitable for the determination of PFOA in sewage and urine.
Cheng
Xiao-yan
XY
Department of Sanitary Technology, West China School of Public Health, Sichuan University, Changdu, China.
Zhang
Ke-rong
KR
Zheng
Bo
B
D002210:Caprylates / Q000032:analysis* / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005466:Fluorocarbons / Q000032:analysis* / Q000652:urine*; D057230:Limit of Detection; D012722:Sewage / Q000032:analysis* / Q000737:chemistry; D014466:Ultraviolet Rays
D002210:Caprylates; D005466:Fluorocarbons; D012722:Sewage; C023036:perfluorooctanoic acid