Combining X-ray and electron-microscopy data to solve crystal structures.
Low-resolution electron-microscopy reconstructions can be used as search models in molecular replacement or may be combined with existing monomeric structures in order to produce multimeric models suitable for molecular replacement. The technique is described in the case of viral and subviral particles as well as in the case of oligomeric proteins.
Navaza
Jorge
J
Laboratoire de Microscopie Electronique Structurale, Institut de Biologie Structurale Jean-Pierre Ebel, 41 Rue Jules Horowitz, F-38027 Grenoble, France. jorge.navaza@ibs.fr
D016960:Agrobacterium tumefaciens / Q000201:enzymology; D020285:Cryoelectron Microscopy / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods*; D019281:Dimerization; D006006:Glycogen Synthase / Q000737:chemistry / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D008958:Models, Molecular; D012984:Software; D015678:Viral Structural Proteins / Q000737:chemistry / Q000648:ultrastructure
C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins; D006006:Glycogen Synthase