Fragments of obestatin as modulators of feed intake, circulating lipids, and stored fat.
Obestatin, shown to reduce feed intake and gain in body weight in rodents, is a very attractive candidate to be used against obesity. In this study, we aimed to investigate the relationship between the primary structure and activity of obestatin. Also of interest to us is a peptide of minimal length that closely mimics obestatin. Towards the same, we synthesized rodent obestatin and three overlapping fragments spanning residues 1-13, 6-18, and 11-23 of obestatin. These peptides subsequent to purification and characterization were tested upon adult male mice for their ability to reduce feed intake and gain in body weight. The N-terminal peptide (residues 1-13) mimicked obestatin the closest. The middle fragment (residues 6-18) significantly reduced epididymal fat without much altering feed intake or body weight.
Nagaraj
ShreeRanga
S
Department of Protein Chemistry and Technology, Central Food Technological Research Institute, Cheluvamba Mansion, Mysore 570020, Karnataka, India.
Peddha
Muthukumar S
MS
Manjappara
Uma V
UV
D000273:Adipose Tissue / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000502:physiology*; D004435:Eating / Q000187:drug effects / Q000502:physiology*; D054439:Ghrelin / Q000008:administration & dosage*; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids / Q000097:blood*; D008297:Male; D051379:Mice; D010446:Peptide Fragments / Q000008:administration & dosage
D054439:Ghrelin; D008055:Lipids; D010446:Peptide Fragments