Abnormal collagen deposition in synovia after collagen type V immunization in rabbits.
Sinovitis in Scleroderma (SSc) is rare, usually aggressive and fully resembles rheumatoid arthritis. Experimental models of SSc have been used in an attempt to understand its pathogenesis. Previous studies done in our laboratory had already revealed the presence of a synovial remodeling process in rabbits immunized with collagen V. To validate the importance of collagen type V and to explore the quantitative relationship between this factor and synovia remodeling as well as the relationship between collagen type V and other collagens, we studied the synovial tissue in immunized rabbits. Rabbits (N=10) were immunized with collagen V plus Freund's adjuvant and compared with animals inoculated with adjuvant only (N=10). Synovial tissues were submitted to histological analysis, immunolocalization to collagen I, III and V and biochemical analysis by eletrophoresis, immunoblot and densitometric method. The synovial tissue presented an intense remodeling process with deposits of collagen types I, III and V after 75 and 120 days of immunization, mainly distributed around the vessels and interstitium of synovial extracellular matrix. Densitometric analysis confirmed the increased synthesis of collagen I, III and V chains (407.69+/-80.31; 24.46+/-2.58; 70.51+/-7.66, respectively) in immunized rabbits when compared with animals from control group (164.91+/-15.67; 12.89+/-1.05; 32+/-3.57) (p<0.0001). We conclude that synovial remodeling observed in the experimental model can reflect the articular compromise present in patients with scleroderma. Certainly, this experimental model induced by collagen V immunization will bring new insights in to pathogenic mechanisms and allow the testing of new therapeutic strategies to ameliorate the prognosis for scleroderma patients.
Ogido
Luciana Tsuzuki Ichicawa
LT
Discipline of Rheumatology, Faculty of Medicine, University of São Paulo, Brazil.
Teodoro
Walcy Rosolia
WR
Velosa
Ana Paula Pereira
AP
de Oliveira
Cristiane Carla
CC
Parra
Edwin Roger
ER
Capelozzi
Vera Luiza
VL
Yoshinari
Natalino Hajime
NH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D024062:Collagen Type V / Q000276:immunology* / Q000378:metabolism*; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000378:metabolism / Q000473:pathology; D005260:Female; D007114:Immunization; D011817:Rabbits; D012595:Scleroderma, Systemic / Q000276:immunology / Q000378:metabolism; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology
D000911:Antibodies, Monoclonal; D024042:Collagen Type I; D024061:Collagen Type III; D024062:Collagen Type V