title: Neuronal nitric oxide synthase contributes to the regulation of hematopoiesis.
abstract: Nitric oxide (NO) signaling is important for the regulation of hematopoiesis. However, the role of individual NO synthase (NOS) isoforms is unclear. Our results indicate that the neuronal NOS isoform (nNOS) regulates hematopoiesis in vitro and in vivo. nNOS is expressed in adult bone marrow and fetal liver and is enriched in stromal cells. There is a strong correlation between expression of nNOS in a panel of stromal cell lines established from bone marrow and fetal liver and the ability of these cell lines to support hematopoietic stem cells; furthermore, NO donor can further increase this ability. The number of colonies generated in vitro from the bone marrow and spleen of nNOS-null mutants is increased relative to wild-type or inducible- or endothelial NOS knockout mice. These results describe a new role for nNOS beyond its action in the brain and muscle and suggest a model where nNOS, expressed in stromal cells, produces NO which acts as a paracrine regulator of hematopoietic stem cells.
authors: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
authors: Peter
authors: P
authors: Krasnov
authors: Tatyana
authors: T
authors: Michurina
authors: Michael A
authors: MA
authors: Packer
authors: Yuri
authors: Y
authors: Stasiv
authors: Naoki
authors: N
authors: Nakaya
authors: Kateri A
authors: KA
authors: Moore
authors: Kenneth E
authors: KE
authors: Drazan
authors: Grigori
authors: G
authors: Enikolopov
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000201:enzymology; D002478:Cells, Cultured; D005260:Female; D006410:Hematopoiesis / Q000502:physiology*; D022781:Hepatocytes / Q000166:cytology / Q000201:enzymology; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D052248:Nitric Oxide Synthase Type I / Q000235:genetics / Q000378:metabolism*; D019899:Paracrine Communication; D013154:Spleen / Q000166:cytology / Q000201:enzymology; D013234:Stem Cells / Q000166:cytology / Q000201:enzymology; D017154:Stromal Cells / Q000166:cytology / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D052248:Nitric Oxide Synthase Type I