Direct acoustic profiling of DNA hybridisation using HSV type 1 viral sequences.
We describe the detection of specific, conserved DNA sequences of herpes simplex virus (HSV) type 1 by means of a novel, high sensitivity acoustic biosensor. Repeated assays on planar and polymeric carboxylic acid- and biotin-presenting surface chemistries enabled statistical comparison of assay specificity and sensitivity and evaluation of assay Z-factor scores. Using a three minute hybridisation with NeutrAvidin capture for signal enhancement, it was possible to detect HSV viral nucleic acids at 5.2 x 10(-11) M concentration.
Uludağ
Yildiz
Y
Akubio Ltd, 181 Cambridge Science Park, Cambridge, UK CB4 0GJ.
Li
Xin
X
Coleman
Heather
H
Efstathiou
Stacey
S
Cooper
Matthew A
MA
D000162:Acoustics; D015374:Biosensing Techniques; D004279:DNA, Viral / Q000032:analysis*; D018259:Herpesvirus 1, Human / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012680:Sensitivity and Specificity
D004279:DNA, Viral