title: Nerve growth factor helps protect retina in experimental retinal detachment.
abstract: OBJECTIVE
  To investigate the expression of nerve growth factor (NGF) and its receptor TrkA on the retina at different time points after retinal detachment (RD) and the protection effect of NGF in experimental RD.
  
  
  METHODS
  Sprague-Dawley rats were used as an RD animal model by injection of 0.1% sodium hyaluronate under the neurosensory retina. The expression of endogenous NGF and its receptor TrkA in the rat retina was detected using immunohistochemistry. The NGF (5 mug/eye) or phosphate-buffered saline were injected separately into vitreous every 4 days after the RD. The rat eyes were then observed at various time points. Morphologic changes were investigated by light microscopy. Retinal gliosis was detected by glial fibrillary acidic protein labeling.
  
  
  RESULTS
  The expression of endogenous NGF and TrkA was upregulated during RD procedure. Most of the NGF-treated retina had a well-organized structure. In NGF-treated RD eyes, the cells were still significantly more numerous than in phosphate-buffered-saline-treated retina. Glial fibrillary acidic protein labeling increased quickly after RD; the NGF-treated retina had less reactive gliosis than the control groups.
  
  
  CONCLUSIONS
  Intravitreal injection of exogenous NGF can protect retinal cells from degeneration in experimental RD. It may exert its protective action by preventing the apoptosis of retinal cells after RD.
authors: Eye Research Institute of Shanghai Jiaotong University, Shanghai, China.
authors: Xiaodong
authors: X
authors: Sun
authors: Xun
authors: X
authors: Xu
authors: Fenghua
authors: F
authors: Wang
authors: Xi
authors: X
authors: Zhang
authors: Patrick C P
authors: PC
authors: Ho
authors: Haiyang
authors: H
authors: Liu
authors: Jin
authors: J
authors: Qian
authors: Zhang
authors: Z
authors: Yu
authors: Hongfen
authors: H
authors: Lu
authors: Weiqi
authors: W
authors: Xu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005911:Gliosis / Q000473:pathology; D007267:Injections; D008297:Male; D020932:Nerve Growth Factor / Q000378:metabolism* / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020917:Receptor, trkA / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D012160:Retina / Q000187:drug effects / Q000473:pathology; D012162:Retinal Degeneration / Q000473:pathology; D012163:Retinal Detachment / Q000473:pathology*; D015854:Up-Regulation / Q000502:physiology; D014822:Vitreous Body
chemical_list: D005904:Glial Fibrillary Acidic Protein; D011994:Recombinant Proteins; D020932:Nerve Growth Factor; D020917:Receptor, trkA