title: Regional adaptations in PSD-95, NGFI-A and secretogranin gene transcripts related to vulnerability to behavioral sensitization to amphetamine in the Roman rat strains.
abstract: Genetically selected for high or low two-way active avoidance, Roman high-avoidance (RHA) and Roman low-avoidance (RLA) rats differ in their central dopaminergic activity, sensation/novelty- and substance-seeking profiles. These animals are, therefore, well suited to identify anatomical and neurochemical concomitants of behavioral sensitization, a phenomenon linked to addictive liability. We submitted inbred RHA (RHA-I), inbred RLA (RLA-I) and Sprague-Dawley-OFA (SD-OFA) rats to a sensitization regimen with amphetamine and studied the behavioral response to an amphetamine challenge after a 2-week withdrawal period. The expression patterns of nerve growth factor inducible clone A (NGFI-A), secretogranin, post-synaptic density protein of 95 Kd (PSD-95), prodynorphin and proenkephalin mRNA were also analyzed using in situ hybridization, after the challenge with amphetamine. RHA-I rats showed stronger sensitization than SD-OFA rats. RLA-I rats did not show sensitization but were hyper-reactive to amphetamine. Expression of behavioral sensitization in RHA-I rats activated secretogranin and PSD-95 mRNA in the nucleus accumbens core. On the other hand, high induction of NGFI-A mRNA in the central amygdala was observed in RLA-I rats when they experienced amphetamine for the first time in the challenge. Our results reveal that 1) the acute locomotor response to amphetamine does not predict vulnerability to behavioral sensitization and 2) differences in vulnerability to sensitization may involve distinctive cellular adaptations at particular brain locations which may be related to addictive vulnerability.
authors: Department of Psychiatry and Forensic Medicine, Institute of Neuroscience, Autonomous University of Barcelona, 08193 Bellaterra, Barcelona, Spain.
authors: M
authors: M
authors: Guitart-Masip
authors: B
authors: B
authors: Johansson
authors: T
authors: T
authors: Cañete
authors: A
authors: A
authors: Fernández-Teruel
authors: A
authors: A
authors: Tobeña
authors: L
authors: L
authors: Terenius
authors: L
authors: L
authors: Giménez-Llort
mesh_terms: D000661:Amphetamine / Q000494:pharmacology*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D002864:Chromogranins / Q000096:biosynthesis / Q000235:genetics*; D000076122:Disks Large Homolog 4 Protein; D004399:Dynorphins / Q000096:biosynthesis / Q000235:genetics; D051766:Early Growth Response Protein 1 / Q000096:biosynthesis / Q000235:genetics*; D004745:Enkephalins / Q000096:biosynthesis / Q000235:genetics; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D009043:Motor Activity / Q000187:drug effects; D009714:Nucleus Accumbens / Q000187:drug effects / Q000378:metabolism; D009838:Oligodeoxyribonucleotides; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D017207:Rats, Sprague-Dawley; D013045:Species Specificity
chemical_list: D000697:Central Nervous System Stimulants; D002864:Chromogranins; D000076122:Disks Large Homolog 4 Protein; C501449:Dlg4 protein, rat; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D004745:Enkephalins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009838:Oligodeoxyribonucleotides; D011498:Protein Precursors; C029992:proenkephalin; D004399:Dynorphins; D000661:Amphetamine