[T-lymphocyte voltage dependent K(+) channel is upregulated in patients with acute coronary syndrome].
OBJECTIVE
To determine current density of voltage-gated potassium channels and Kv1.3 express in T-lymphocyte derived from patients with acute coronary syndrome (ACS).


METHODS
Peripheral blood mononuclear cells were collected from 12 patients with ACS and 10 control donors. Whole-cell patch clamp technique was used to record the outward K(+) currents (IK) and western blots technique was used to detect the express of Kv1.3 protein in lymphocyte.


RESULTS
(1) The current density of voltage-gated potassium channel was significantly higher in ACS patients [(269 +/- 94) pA/pF] than in controls [(191 +/- 64) pA/pF, P < 0.01] while membrane capacitance was similar between the two groups. (2) Kv1.3 protein expression was also significantly increased in ACS patients than in controls (P < 0.01).


CONCLUSIONS
The lymphocyte voltage-gated potassium channel is upregulated in patients with ACS suggesting a role of Kv activation in the pathophysiology of ACS.
Guo
Li-fen
LF
Department of Cardiology, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
Zhang
Cun-tai
CT
Wu
Jie
J
Liu
Nian
N
Sun
Li-ping
LP
Liu
Jun
J
Ma
Jin
J
D054058:Acute Coronary Syndrome / Q000378:metabolism*; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D051664:Kv1.3 Potassium Channel / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018408:Patch-Clamp Techniques; D013601:T-Lymphocytes / Q000378:metabolism*
D051664:Kv1.3 Potassium Channel