CD8+FOXP3+T cells from renal transplant recipients in quiescence induce immunoglobulin-like transcripts-3 and -4 on dendritic cells from their respective donors.
In previous studies, CD8+FOXP3+ T cells have been shown to upgrade the inhibitory receptors on dendritic cells (DC) in heart transplantation patients. The inhibitory receptor were immunoglobulin-like transcripts (ILT)-3 and ILT-4, which were highly expressed on tolerogenic dendritic cells. Our study focused on the CD8+FOXP3+ T cells from allogeneic renal transplant recipients, seeking to dissert their function in inducing ILT-3- and ILT-4-expressing dendritic cells. We analyzed 11 quiescent renal transplant recipients and their respective donors. Reverse transcriptase polymerase chain reaction analysis showed that the levels of Foxp3 mRNA in CD8+ T cells after renal transplantation were significantly higher than those in CD8+ T cells before transplantation. We further investigated the capacity of allospecific CD8+FOXP3+ T cells to induce upregulation of the inhibitory receptors ILT-3 and ILT-4 on DC. Data from FACS showed that CD8+FOXP3+ T cells induced greater expression of ILT-3 and ILT-4 on DC in an allospecific fashion. This study confirmed that Foxp3 gene expression was enhanced among quiescent renal transplant patients, suggesting that CD8+FOXP3+ T cells play an important role in unresponsiveness related to upregulated ILT-3 and ILT-4 receptors on DC.
Zhou
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Institute of Hematology, Department of Urology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Hubei, China.
Wang
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ZD
Zhu
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You
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Y
Zou
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P
D016827:CD8 Antigens / Q000235:genetics*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology*; D051858:Forkhead Transcription Factors / Q000235:genetics*; D006801:Humans; D007108:Immune Tolerance; D016030:Kidney Transplantation / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014019:Tissue Donors; D014158:Transcription, Genetic; D014184:Transplantation, Homologous
D016827:CD8 Antigens; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D012333:RNA, Messenger