title: Proteome analysis of Streptococcus suis serotype 2.
abstract: Outbreaks in humans, caused by Streptococcus suis serotype 2 (SS2), were reported in 1998 and 2005 in China. However, the mechanism of SS2-associated infection remains unclear. For the first time, a 2-D gel approach combined with MS was used to establish a comprehensive 2-D reference map for aiding our understanding of the pathogenicity of SS2. The identification of 694 out of 834 processed spots revealed 373 proteins. Most of the identified proteins were located in the cytoplasm and were involved in energy metabolism, protein synthesis, and cellular processes. Proteins that were abundant in the 2-DE gels could be linked mainly to housekeeping functions in carbohydrate metabolism, protein quality control and translation. 2-DE of secretory proteins was performed using IPG strips of pH 4-7. Among the 102 protein spots processed, 87 spots representing 77 proteins were successfully identified. Some virulence-associated proteins of SS2 were found, including arginine deiminase, ornithine carbamoyl-transferase, carbamate kinase, muramidase-released protein precursor, extracellular factor, and suilysin. Enolase and endopeptidase have been proposed as putative virulence-associated factors in this study. The 2-D reference map might provide a powerful tool for analyzing the virulence factor and the regulatory network involved in the pathogenicity of this microorganism.
authors: State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, No. 20 Dongda Street, Beijing, People's Republic of China.
authors: Hong-Bo
authors: HB
authors: Jing
authors: Jing
authors: J
authors: Yuan
authors: Jie
authors: J
authors: Wang
authors: Yuan
authors: Y
authors: Yuan
authors: Li
authors: L
authors: Zhu
authors: Xian-Kai
authors: XK
authors: Liu
authors: Yu-Ling
authors: YL
authors: Zheng
authors: Kai-Hua
authors: KH
authors: Wei
authors: Xue-Min
authors: XM
authors: Zhang
authors: Hong-Ran
authors: HR
authors: Geng
authors: Qing
authors: Q
authors: Duan
authors: Shu-Zhang
authors: SZ
authors: Feng
authors: Rui-Fu
authors: RF
authors: Yang
authors: Wu-Chun
authors: WC
authors: Cao
authors: Heng-Liang
authors: HL
authors: Wang
authors: Yong-Qiang
authors: YQ
authors: Jiang
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome / Q000032:analysis*; D011544:Pseudogenes; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017011:Streptococcus suis / Q000235:genetics*; D037521:Virulence Factors / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D037521:Virulence Factors