Dual-probe system using pyrenylmethyl-modified amino-LNA for mismatch detection.
A dual-probe containing pyrenylmethyl amino-LNA has been developed for sensitive mismatch detection. While hybridization with complementary DNA/RNA results in very strong excimer signals, exposure to singly mismatched DNA/RNA targets results in significantly decreased excimer emission.
Umemoto
Tadashi
T
Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. tumemoto@riken.jp
Hrdlicka
Patrick J
PJ
Babu
B Ravindra
BR
Wengel
Jesper
J
D000588:Amines / Q000737:chemistry; D020137:Base Pair Mismatch; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods*; D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D011721:Pyrenes / Q000737:chemistry; D012313:RNA / Q000737:chemistry / Q000235:genetics
D000588:Amines; D015341:Nucleic Acid Probes; D009841:Oligonucleotides; D011721:Pyrenes; C477371:locked nucleic acid; D012313:RNA; D004247:DNA