title: Characterization of Shiga toxin-producing Escherichia coli isolates associated with two multistate food-borne outbreaks that occurred in 2006.
abstract: Shiga toxin-producing Escherichia coli isolates from two 2006 outbreaks were compared to other O157:H7 isolates for virulence genotype, biofilm formation, and stress responses. Spinach- and lettuce-related-outbreak strains had similar pulsed-field gel electrophoresis patterns, and all carried both stx2 and stx2c variant genes. Cooperative biofilm formation involving an E. coli O157:H7 strain and a non-O157:H7 strain was also demonstrated.
authors: Microbial Food Safety Research Unit, Eastern Regional Research Center, ARS, USDA, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA. gaylen.uhlich@ars.usda.gov
authors: G A
authors: GA
authors: Uhlich
authors: J R
authors: JR
authors: Sinclair
authors: N G
authors: NG
authors: Warren
authors: W A
authors: WA
authors: Chmielecki
authors: P
authors: P
authors: Fratamico
mesh_terms: D001483:Base Sequence; D018441:Biofilms / Q000254:growth & development; D003092:Colitis / Q000453:epidemiology* / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005516:Food Microbiology; D005838:Genotype; D006801:Humans; D018545:Lactuca / Q000382:microbiology; D008969:Molecular Sequence Data; D010414:Pennsylvania / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012703:Serotyping; D022621:Shiga Toxin / Q000235:genetics / Q000378:metabolism*; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D018724:Spinacia oleracea / Q000382:microbiology; D014774:Virulence
chemical_list: D017931:DNA Primers; D022621:Shiga Toxin