Effect of hydroxyurea on procyclic Trypanosoma brucei: an unconventional mechanism for achieving synchronous growth.
Procyclic Trypanosoma brucei cells were synchronized with 0.2 mM hydroxyurea. The cells did not arrest at the G(1)/S boundary but proceeded through one round of replication and arrested near the end of S phase. The mitochondrial genome (kinetoplast DNA network) replicated, forming two progeny networks, but the repair of minicircle gaps was inhibited.
Chowdhury
Arnab Roy
AR
Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
Zhao
Zhixing
Z
Englund
Paul T
PT
D000818:Animals; D004261:DNA Replication / Q000187:drug effects*; D018105:DNA, Kinetoplast / Q000235:genetics*; D016193:G1 Phase / Q000187:drug effects*; D006918:Hydroxyurea / Q000494:pharmacology*; D016196:S Phase / Q000187:drug effects*; D014346:Trypanosoma brucei brucei / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
D018105:DNA, Kinetoplast; D006918:Hydroxyurea