A non-invasive technique for rapid extraction of DNA from fish scales.
DNA markers are being increasingly used in studies related to population genetics and conservation biology of endangered species. DNA isolation for such studies requires a source of biological material that is easy to collect, non-bulky and reliable. Further, the sampling strategies based on non-invasive procedures are desirable, especially for the endangered fish species. In view of above, a rapid DNA extraction method from fish scales has been developed with the use of a modified lysis buffer that require about 2 hr duration. This methodology is non-invasive, less expensive and reproducible with high efficiency of DNA recovery. The DNA extracted by this technique, have been found suitable for performing restriction enzyme digestion and PCR amplification. Therefore, the present DNA extraction procedure can be used as an alternative technique in population genetic studies pertaining to endangered fish species. The technique was also found equally effective for DNA isolation from fresh, dried and ethanol preserved scales.
Kumar
Ravindra
R
National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow 226 002, India. ravindrakumar@scientist.com
Singh
Poonam Jayant
PJ
Nagpure
N S
NS
Kushwaha
Basdeo
B
Srivastava
S K
SK
Lakra
W S
WS
D000818:Animals; D003247:Conservation of Natural Resources; D004247:DNA / Q000302:isolation & purification*; D004784:Environmental Monitoring / Q000379:methods; D005399:Fishes / Q000235:genetics*; D034582:Integumentary System; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
D004247:DNA