Targeted folic acid-PEG nanoparticles for noninvasive imaging of folate receptor by MRI.
The surface of superparamagnetic iron oxide nanoparticles (SPIO) with different molecular weight of poly(ethylene glycol) (PEG) and folic acid (FA) were synthesized. The SPIO-PEG-FA nanoparticles are well-dispersed and have good stability in various pH solutions. The lack of hysterestis and remanence at ambient temperatures is characteristic of superparamagnetic materials for SPIO-PEG-FA. The uptake by macrophage for SPIO-PEG-FA is lower than that of Feridex I.V. even at higher concentration. Internalization of SPIO-PEG-FA in targeted cells (KB cells) was observed by flow-cytometric analysis and in vitro MR imaging. The intensity change of positive KB cell tumor (-20 to 25%) is significantly lower than that of negative HT-1080 cell tumor from precontrast to postcontrast images of the tumor by in vivo MR imaging. These preliminary results demonstrated that SPIO-PEG-FA have the ability to target folate receptor.
Chen
Ting-Jung
TJ
Faculty of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Cheng
Tsan-Hwang
TH
Hung
Yu-Chin
YC
Lin
Kuei-Tang
KT
Liu
Gin-Chung
GC
Wang
Yun-Ming
YM
D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry*; D003287:Contrast Media / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D005434:Flow Cytometry; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D007624:KB Cells; D008264:Macrophages / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D051379:Mice; D016513:Mice, SCID; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry*; D011956:Receptors, Cell Surface / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D023041:Xenograft Model Antitumor Assays
D002352:Carrier Proteins; D003287:Contrast Media; D005290:Ferric Compounds; D058973:Folate Receptors, GPI-Anchored; D011956:Receptors, Cell Surface; C000499:ferric oxide; D011092:Polyethylene Glycols; D005492:Folic Acid