Effect of surface conjugation chemistry on the sensitivity of microcantilever sensors.
Microcantilever sensors are an offshoot of atomic force microscopy and are useful tools for effectively detecting a target biomolecule. The recognition of the target molecule on the biosensor is based on the physical bending of the microcantilever, which is driven by a specific molecular interaction between the target molecule and the sensor surface. In this study, to enhance the sensitivity of the microcantilever sensor, the sensor surface was modified through a surface conjugation method using self-assembled monolayers (SAMs) and heterobifunctional cross-linkers. After the surface modification of the microcantilever sensor, the sensitivity for L-cysteine was recorded. The detection of L-cysteine was influenced by the active site and the molecular size of the cross-linked compound attached onto the surface of the microcantilever.
Kim
Dae Jung
DJ
Department of Chemistry and Biochemistry, Texas Tech University, Texas 79409-1061, USA.
Weeks
Brandon L
BL
Hope-Weeks
Louisa J
LJ
D015374:Biosensing Techniques / Q000379:methods*; D002857:Chromium; D003543:Cysteamine; D003545:Cysteine / Q000032:analysis / Q000378:metabolism; D006046:Gold; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds; D013499:Surface Properties; D008063:Thioctic Acid / Q000031:analogs & derivatives
D013438:Sulfhydryl Compounds; D002857:Chromium; D003543:Cysteamine; D008063:Thioctic Acid; D006046:Gold; D003545:Cysteine