Inhibition of human ovarian tumor growth by cytokine-induced killer cells.
Despite the recent improvement in the treatment of ovarian cancer, this disease is still leading cause of cancer death in women. In this study, the anti-tumor activity of cytokine-induced killer (CIK) cells against human ovarian cancer was evaluated in vitro and in vivo. Although CD3+CD56+ cells were rare in fresh human peripheral blood mononuclear cells, they could expand more than 1,000-fold on day 14 in the presence of anti-CD3 antibody plus IL-2. At an effector-target cell ratio of 30:1, CIK cells destroyed 45% of SK-OV-3 human ovarian cancer cells, which was determined by the 51Cr-release assay. In addition, CIK cells at a dose of 23 million cells per mouse inhibited 73% of SK-OV-3 tumor growth in nude mouse xenograft assay. This study suggests that CIK cells may be used as an adoptive immunotherapy for patients with ovarian cancer.
Kim
Hwan Mook
HM
Bioevaluation Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang, Chungbuk 363-883, Korea.
Kang
Jong Soon
JS
Lim
Jaeseung
J
Park
Song-Kyu
SK
Lee
Kiho
K
Yoon
Yeo Dae
YD
Lee
Chang Woo
CW
Lee
Ki Hoon
KH
Han
Gyoonhee
G
Yang
Kyu-Hwan
KH
Kim
Yeon Jin
YJ
Kim
Youngsoo
Y
Han
Sang-Bae
SB
D000818:Animals; D045744:Cell Line, Tumor; D016207:Cytokines / Q000494:pharmacology*; D005260:Female; D006801:Humans; D016219:Immunotherapy, Adoptive; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D010051:Ovarian Neoplasms / Q000473:pathology / Q000628:therapy*
D016207:Cytokines