title: Structural basis of the initial binding of tRNA(Ile) lysidine synthetase TilS with ATP and L-lysine.
abstract: In the bacterial genetic-code system, the codon AUA is decoded as isoleucine by tRNA(Ile)(2) with the lysidine residue at the wobble position. Lysidine is derived from cytidine, with ATP and L-lysine, by tRNA(Ile) lysidine synthetase (TilS), which is an N-type ATP pyrophosphatase. In this study, we determined the crystal structure of Aquifex aeolicus TilS, complexed with ATP, Mg2+, and L-lysine, at 2.5 A resolution. The presence of the TilS-specific subdomain causes the active site to have two separate gateways, a large hole and a narrow tunnel on the opposite side. ATP is bound inside the hole, and L-lysine is bound at the entrance of the tunnel. The conserved Asp36 in the PP-motif coordinates Mg2+. In these initial binding modes, the ATP, Mg2+, and L-lysine are held far apart from each other, but they seem to be brought together for the reaction upon cytidine binding, with putative structural changes of the complex.
authors: Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors: Mitsuo
authors: M
authors: Kuratani
authors: Yuka
authors: Y
authors: Yoshikawa
authors: Yoshitaka
authors: Y
authors: Bessho
authors: Kyoko
authors: K
authors: Higashijima
authors: Takeshi
authors: T
authors: Ishii
authors: Rie
authors: R
authors: Shibata
authors: Seizo
authors: S
authors: Takahashi
authors: Katsuhide
authors: K
authors: Yutani
authors: Shigeyuki
authors: S
authors: Yokoyama
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry / Q000378:metabolism*; D008239:Lysine / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D000255:Adenosine Triphosphate; D000604:Amino Acyl-tRNA Synthetases; D008239:Lysine