title: [Effect of Ganoderma lucidum polysaccharides peptide on invasion of human lung carcinoma cells in vitro].
abstract: OBJECTIVE
  To find out the effects of Ganoderma lucidum polysaccharides peptide (Gl-PP) on the invasion of the human lung carcinoma cell (PG cell).
  
  
  METHODS
  PG cells were pretreated with different concentration Gl-PP in vitro, using cell proliferation assay, cell migration assay, adhesion assay, zymography and RT-PCR, then the effects of Gl-PP on proliferation, motility, adhesion and MMP-9 activity and mRNA expression of PG cells were investigated in vitro.
  
  
  RESULTS
  Gl-PP did not directly inhibit PG cell proliferation in vitro. However, pretreated with Gl-PP, PG cells motility was inhibited significantly. PG cells adhesion was also inhibited. The activity of MMP-9 was inhibited in a dose-dependent manner, and the inhibited ratio was 41.53% at a dose of 100 mg/L Gl-PP. The mRNA expression of MMP-9 of pretreated PG cells was inhibited.
  
  
  CONCLUSIONS
  Gl-PP could suppress invasion of human lung carcinoma cells in vitro.
authors: Department of Pharmacology, Peking University School of Basic Medical Sciences, Beijing 100083, China.
authors: Qi Zhen
authors: QZ
authors: Cao
authors: Shu Qian
authors: SQ
authors: Lin
authors: Sai Zhen
authors: SZ
authors: Wang
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D011509:Proteoglycans / Q000494:pharmacology*; D020940:Reishi
chemical_list: D011509:Proteoglycans; C093216:polysaccharide peptide; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9