title: Mass spectrometry based methods for analysis of nucleosides as antiviral drugs and potential tumor biomarkers.
abstract: The intracellular analysis of the phosphorylated metabolites of some anti-HIV nucleosides by liquid chromatography or capillary electrophoresis coupled with tandem mass spectrometry (LC-MS/MS or CE-MS/MS) has been realized on human peripheral blood mononuclear cells (PBMC), with limit of quantitation (LOQ) that allow them to be quantitated intracellularly. We described also the analysis of modified urinary nucleosides as potential tumor biomarkers.
authors: Institut de Chimie Organique et Analytique, UMR CNRS 6005, Université d'Orléans, 45067 Orléans Cedex 2, France. luigi.agrofoglio@univ-orleans.fr
authors: Luigi A
authors: LA
authors: Agrofoglio
authors: Vincent
authors: V
authors: Bezy
authors: Patrick
authors: P
authors: Chaimbault
authors: Raphaël
authors: R
authors: Delépée
authors: Boutaina
authors: B
authors: Rhourri
authors: Philippe
authors: P
authors: Morin
mesh_terms: D019380:Anti-HIV Agents / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis / Q000652:urine*; D002853:Chromatography, Liquid; D019075:Electrophoresis, Capillary; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D009705:Nucleosides / Q000032:analysis* / Q000652:urine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D019380:Anti-HIV Agents; D014408:Biomarkers, Tumor; D009705:Nucleosides