Integrating spatially resolved three-dimensional MALDI IMS with in vivo magnetic resonance imaging.
We have developed a method for integrating three dimensional-volume reconstructions of spatially resolved matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) ion images of whole mouse heads with high-resolution images from other modalities in an animal-specific manner. This approach enabled us to analyze proteomic profiles from MALDI IMS data with corresponding in vivo data provided by magnetic resonance imaging.
Sinha
Tuhin K
TK
Department of Radiology and Radiological Sciences, Vanderbilt University, 1161 21st Avenue South, Nashville, Tennessee 37232, USA. tk.sinha@vanderbilt.edu
Khatib-Shahidi
Sheerin
S
Yankeelov
Thomas E
TE
Mapara
Khubaib
K
Ehtesham
Moneeb
M
Cornett
D Shannon
DS
Dawant
Benoit M
BM
Caprioli
Richard M
RM
Gore
John C
JC
D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000378:metabolism*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D010449:Peptide Mapping / Q000379:methods*; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D018511:Systems Integration; D014018:Tissue Distribution
D020543:Proteome