[Proteomics study of the serum biomarkers in tongue cancer].
OBJECTIVE
To search for specific serum biomarkers associated with tongue cancer by means of the serum proteomics technology.


METHODS
The tongue cancer cells of human tongue cancer cell line Tca8113 were subcutaneously inoculated into nude mice, while control nude mice were injected with phosphate-buffered saline. Serums from these two group of mice were collected for proteomic analysis using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS).


RESULTS
Comparing the serum 2-DE maps from the tumor-bearing mice with those produced from control mice, we found that squamous cell carcinoma antigen 1 was over expressed only in the serum of tumor-bearing mice.


CONCLUSIONS
The squamous cell carcinoma antigen 1 may be of great potential as the biomarker of tongue cancer and as the potential therapeutic target for gene therapy.
Huang
Xin
X
Department of Oral and Maxillofacial Surgery, Faculty of Stomatology, Capital University of Medical Sciences, Beijing 100050, China. huangxin_291@hotmail.com
Kang
Fei-Wu
FW
Li
Long-Jiang
LJ
Wen
Yu-Ming
YM
D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000473:pathology; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D013058:Mass Spectrometry; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D040901:Proteomics / Q000379:methods; D014062:Tongue Neoplasms / Q000097:blood* / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
D014408:Biomarkers, Tumor