5-methylcytosine-selective osmium oxidation.
Methylcytosine was efficiently and easily modified through osmium oxidation. Methylcytosine-selective oxidation is applicable to detection of the cytosine methylation status at a specific site of a long sequence using the formation of a bulge structure by hybridization with a guide DNA.
Okamoto
Akimitsu
A
Frontier Research System, RIKEN, Wako, Saitama, Japan. aki-okamoto@riken.jp
D044503:5-Methylcytosine / Q000737:chemistry*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D019175:DNA Methylation; D009992:Osmium / Q000737:chemistry*; D010084:Oxidation-Reduction
D009992:Osmium; D044503:5-Methylcytosine; D004247:DNA