title: Evaluation of different chemical strategies for conjugation of oligonucleotides to peptides.
abstract: We developed novel assays for high-throughput detection of one or many kinases or proteases. The assays use hundreds of different peptide substrates, each covalently linked to an oligonucleotide tag. After incubation with sample, the pool of substrates is hybridized to a microarray containing oligonucleotides complementary to the tag sequences. We screened several specific chemistries for the conjugation based on the following criteria: easy derivatization of oligonucleotides and peptides; high efficiency of the conjugation reaction; good stability of the conjugates; and satisfactory conjugate performance in our assays. We have validated selected method during the successful generation of thousands oligonucleotide-peptide conjugates.
authors: Illumina Inc., San Diego, CA 92121, USA.
authors: Igor A
authors: IA
authors: Kozlov
authors: Peter C
authors: PC
authors: Melnyk
authors: John P
authors: JP
authors: Hachmann
authors: David L
authors: DL
authors: Barker
authors: Michal
authors: M
authors: Lebl
authors: Chanfeng
authors: C
authors: Zhao
mesh_terms: D004247:DNA / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000032:analysis* / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D010770:Phosphotransferases / Q000032:analysis* / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D009841:Oligonucleotides; D010455:Peptides; D004247:DNA; D010770:Phosphotransferases; D010447:Peptide Hydrolases