[Separation and determination of oleanolic acid and ursolic acid from Cornus officinalis by capillary electrophoresis].
A cycledextrin-modified micellar electrokinetic chromatography technique for separating and determination oleanolic acid and ursolic acid in the fruit of Cornus officinalis was developed. The two bio-active components was completely separated using a buffer containing 60 mmol/L SDS, 2.5 mmol/L NaH2PO4, 2.5 mmol/L Na2B4O7, 8 mmol/L DM beta-CD,8 mmol/L beta-CD and 30% (v/v) 2-propanol. The correlation cofficients of the linear calibration graphs for oleanolic acid and ursolic acid are 0. 9996 and 0.9995, respectively. The effects of SDS, 2-propanol, dimethyl-beta-cyclodextrin, beta-cyclodextrin, NaH2PO4, Na2B4O7 and pH on separation were investigated.
Wang
Rui
R
Beijing University of Chinese Medicine, Beijing 100102, China.
Wang
Shu-mei
SM
Liang
Sheng-wang
SW
Xu
Guo-wang
GW
Weng
Qian-feng
QF
D019840:2-Propanol / Q000737:chemistry; D029768:Cornus / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D014315:Triterpenes / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D047392:beta-Cyclodextrins / Q000737:chemistry; D000097245:Ursolic Acid
D019840:2-Propanol; D009828:Oleanolic Acid; D012967:Sodium Dodecyl Sulfate; D014315:Triterpenes; D047392:beta-Cyclodextrins; D000097245:Ursolic Acid