title: Dynamic gene expression of Lin-28 during embryonic development in mouse and chicken.
abstract: The Caenorhabditis elegans heterochronic gene lin-28 regulates developmental timing in the nematode trunk. We report the dynamic expression patterns of Lin-28 homologues in mouse and chick embryos. Whole mount in situ hybridization revealed specific and intriguing expression patterns of Lin-28 in the developing mouse and chick limb bud. Mouse Lin-28 expression was detected in both the forelimb and hindlimb at E9.5, but disappeared from the forelimb at E10.5, and finally from the forelimb and hindlimb at E11.5. Chicken Lin-28, which was first detected in the limb primordium at stage 15/16, was also downregulated as the stage proceeded. The amino acid sequences of mouse and chicken Lin-28 genes are highly conserved and the similar expression patterns of Lin-28 during limb development in mouse and chicken suggest that this heterochronic gene is also conserved during vertebrate limb development.
authors: Department of Systems Biomedicine, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo, Japan.
authors: Shigetoshi
authors: S
authors: Yokoyama
authors: Megumi
authors: M
authors: Hashimoto
authors: Hirohito
authors: H
authors: Shimizu
authors: Hiroe
authors: H
authors: Ueno-Kudoh
authors: Kenta
authors: K
authors: Uchibe
authors: Ichiro
authors: I
authors: Kimura
authors: Hiroshi
authors: H
authors: Asahara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000096:biosynthesis / Q000235:genetics*; D002642:Chick Embryo; D017124:Conserved Sequence; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D018878:Limb Buds / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D011247:Pregnancy; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D030161:Avian Proteins; C488352:Lin-28 protein, mouse; D016601:RNA-Binding Proteins