Identification of two E-boxes that negatively modulate the activity of MyoD on the alpha-sarcoglycan core promoter.
The alpha-SG promoter is composed of a plethora of cis-regulatory elements, whose individual contribution to alpha-SG gene expression modulation remains unknown. We have identified a negative regulatory element in the alpha-SG distal promoter including two conserved E-boxes (E1 and E2), which interact with MyoD. We found that E1 and E2 negatively modulate the transactivation potential of MyoD on the alpha-SG core promoter. Moreover, such negative effect is mainly mediated by E2, which is surrounded by conserved nucleotides conferring MyoD binding capacity. Our results suggest that modulation of MyoD activity by E1, and particularly E2, contributes to the negative regulation of alpha-SG gene expression during myogenic differentiation.
Delgado-Olguín
Paul
P
Unidad de Investigación Médica en Genética Humana, Hospital de Pediatría, Centro Médico Nacional Siglo XXI-IMSS, Col. Doctores, Delegación Cuauhtémoc, México DF 06725, México.
Hernández-Hernández
J Manuel
JM
Salamanca
Fabio
F
Recillas-Targa
Félix
F
Coral-Vázquez
Ramón M
RM
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017124:Conserved Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism; D024721:E-Box Elements; D006801:Humans; D051379:Mice; D024510:Muscle Development / Q000235:genetics; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D049031:Sarcoglycans / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D015533:Transcriptional Activation; D014162:Transfection
D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; D049031:Sarcoglycans; D004247:DNA