title: Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 7.
abstract: Crystals of baculovirus-expressed adeno-associated virus serotype 7 capsids diffract X-rays to approximately 3.0 A resolution. The crystals belong to the rhombohedral space group R3, with unit-cell parameters a = 252.4, c = 591.2 A in the hexagonal setting. The diffraction data were processed and reduced to an overall completeness of 79.0% and an R(merge) of 12.0%. There are three viral capsids in the unit cell. The icosahedral threefold axis is coincident with the crystallographic threefold axis, resulting in one third of a capsid (20 monomers) per crystallographic asymmetric unit. The orientation of the viral capsid has been determined by rotation-function searches and is positioned at (0, 0, 0) by packing considerations.
authors: Department of Biochemistry and Molecular Biology, McKnight Brain Institute, Center for Structural Biology, University of Florida, Gainesville, FL 32610, USA.
authors: Odayme
authors: O
authors: Quesada
authors: Brittney
authors: B
authors: Gurda
authors: Lakshmanan
authors: L
authors: Govindasamy
authors: Robert
authors: R
authors: McKenna
authors: Erik
authors: E
authors: Kohlbrenner
authors: George
authors: G
authors: Aslanidi
authors: Sergei
authors: S
authors: Zolotukhin
authors: Nicholas
authors: N
authors: Muzyczka
authors: Mavis
authors: M
authors: Agbandje-McKenna
mesh_terms: D036022:Capsid Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D003460:Crystallization; D000229:Dependovirus / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008854:Microscopy, Electron; D014961:X-Ray Diffraction
chemical_list: D036022:Capsid Proteins