[Effect of human bone marrow mesenchymal stem cells on T lymphocyte killing K562 cells].
The objective of this study was to investigate the effect of human bone marrow mesenchymal stem cells (MSCs) on T lymphocyte killing K562 cells. MSCs were isolated from bone marrow and cultured, T cells were harvested by using nylon column method from peripheral blood. The T cells were co-cultured with MSCs, the phenotype expressions of T cell subsets were detected by flow cytometry. Killing effects of T cells (culture alone and co-culture with MSCs) on K562 cells were detected by LDH, expressions of IFN-gamma and IL-4 were detected by ELISA. The results showed that after T cells were co-cultured with MSCs for three days, the proportion of CD4+ and CD4+CD25+ T cells raised significantly (p<0.05) as compared with group of culture alone, but the proportion of CD8+ T cell were not significantly changed (p>0.05). In group of T cells co-cultured with MSCs, killing effects of T cells on K562 cells weakened, at the same time, expression of IFN-gamma decreased while expression of IL-4 increased. It is concluded that the MSCs weaken killing effects of T cells on K562 cells, which associates with increase of CD4+CD25+ T cell subsets and changes of IFN-gamma and IL-4 levels.
Si
Zhi-Gang
ZG
Department of Hematology, General Hospital of PLA Lanzhou Military Area, Lanzhou 730050, China.
Bai
Hai
H
Wang
Cun-Bang
CB
Xue
Zhi-Wen
ZW
Wang
Qian
Q
Wu
Tao
T
Yang
Xiao-Liang
XL
D001854:Bone Marrow Cells / Q000166:cytology; D015704:CD4 Antigens / Q000276:immunology; D018920:Coculture Techniques; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D015847:Interleukin-4 / Q000378:metabolism; D020014:K562 Cells; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D015704:CD4 Antigens; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D015847:Interleukin-4; D007371:Interferon-gamma