BMI-1 autoantibody in serum as a new potential biomarker of nasopharyngeal carcinoma.
BMI-1 is overexpressed in a variety of cancers, which can activate the immune system to produce antibodies in tumor tissues. In this study, we isolated phage expressing BMI-1 protein by screening of a mixture of nasopharyngeal carcinoma (NPC) cDNA T7 phage library and found that the antibody against BMI-1 was elevated in the sera from NPC patients. BMI-1 mRNA was overexpressed at different levels in seven NPC cell lines compared with normal nasopharyngeal epithelial cell line NP69. Histochemistry showed that patient sera were more reactive with BMI-1 than normal sera. Antibody affinity assay using sera from 40 NPC patients and 54 controls showed that BMI-1 antibody was significantly greater in patient sera than in normal controls (patient 0.791 +/- 0.025 and normal 0.488 +/- 0.042; P < 0.001) and the BMI-1 autoantibody be significantly related with the progress of NPC (Benign versus LNPC P=0.001; LNPC versus MNPC P=0.047). Analysis of the results with logistic regression and receiver operating characteristics (ROC) curves showed that BMI-1 antibody was a modest marker for NPC (sensitivity 0.74 and specificity 0.73; AUC = 0.8044). The showed that BMI-1 antibody as a potential marker of NPC may be rational, and could have diagnostic and prognostic value.
Tong
Yong-Qing
YQ
Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Changsha, Hunan, PR China.
Liu
Bei
B
Huang
Jian
J
Liu
Yan
Y
Guo
Feng-Jie
FJ
Zhou
Guo-Hua
GH
Xie
Ping-Li
PL
Li
Yue-Hui
YH
Hu
Jin-Yue
JY
Li
Guan-Cheng
GC
D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics* / Q000276:immunology*; D063150:Polycomb Repressive Complex 1; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000276:immunology*; D012372:ROC Curve; D012016:Reference Values; D012044:Regression Analysis; D012097:Repressor Proteins / Q000235:genetics* / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001323:Autoantibodies; C069077:BMI1 protein, human; D015415:Biomarkers; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D063150:Polycomb Repressive Complex 1