Molecularly imprinted polymer using beta-cyclodextrin as functional monomer for the efficient recognition of bilirubin.
Bilirubin (BR) imprinted polymer was successfully prepared using supramolecular host compound beta-cyclodextrin (beta-CD) as functional monomer. The adsorption equilibrium was attained in about 4 h, which indicated that the adsorption kinetics was comparatively fast. The results of adsorption and selectivity experiments indicated that BR-imprinted beta-CD polymer was able to bind BR specifically and reversibly. The specific recognition of BR-imprinted beta-CD polymer for BR may be due to the cooperative effects of inclusion interaction and hydrogen bonding. This BR-imprinted beta-CD polymer was further applied to eliminate BR in human serum sample. It was verified that the binding specificity of the BR-imprinted polymer for BR was essentially sufficient in the presence of other compounds coexisting in serum sample. Therefore, as a reusable material possessing high affinity and selectivity, BR-imprinted beta-CD polymer has a potential application perspective as a clinical hemoperfusion material.
Yang
Yu
Y
Beijing National Laboratory for Molecular Sciences, The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry, Peking University, Beijing 100871, China.
Long
Yuanyuan
Y
Cao
Qing
Q
Li
Kean
K
Liu
Feng
F
D000327:Adsorption; D001663:Bilirubin / Q000097:blood* / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D054802:Molecular Imprinting / Q000379:methods*; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013739:Testosterone / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
D011108:Polymers; D047392:beta-Cyclodextrins; D013739:Testosterone; D001663:Bilirubin