Intravital multi-photon microscopy reveals several levels of heterogeneity in endocytic uptake by mouse renal proximal tubules.
Understanding renal function requires one to integrate the structural complexity of kidney nephrons and the dynamic nature of their cellular processes. Multi-photon fluorescence microscopy is a state-of-the-art imaging technique for in vivo analysis of kidney tubules structure and function in real time. This study presents visual evidence for several levels of heterogeneity of proximal tubular endocytic uptake in the superficial renal mouse cortex and illustrates the potential of multi-photon microscopy for providing a comprehensive and dynamic portrayal of renal function.
Caplanusi
A
A
Cell Biology Unit (CELL), Université catholique de Louvain Medical School and de Duve Institute, Brussels, Belgium.
Parreira
K S
KS
Lima
W Rezende
WR
Marien
B
B
Van Der Smissen
P
P
de Diesbach
P
P
Devuyst
O
O
Courtoy
P J
PJ
D000818:Animals; D004705:Endocytosis; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D036641:Microscopy, Fluorescence, Multiphoton; D014018:Tissue Distribution
D005456:Fluorescent Dyes