Single hollow fiber SLM extraction of polyamines followed by tosyl chloride derivatization and HPLC determination.
Determination of polyamines in biological fluids possesses medical diagnostic relevance. Despite the vast panel of analytical methods developed for polyamines they are not applied in routine clinical usage, mainly due to the time and labor consuming sample preparation step and complicated derivatization procedures. Thus, new simpler methods are needed. This paper describes a single hollow fiber SLM extraction method of polyamines followed by simple pre-column derivatization with tosyl chloride and HPLC-UV analysis. The influence of different parameters such as the extraction time, organic phase composition, acceptor pH, donor pH, acceptor volume, donor volume and stirring speed on the transport parameters and enrichment was studied and discussed. The optimized method was applied to real matrices such as urine and plasma.
Dziarkowska
Katarzyna
K
Institute of Chemistry, Opole University, Oleska 48, 45-052 Opole, Poland.
Jönsson
Jan Ake
JA
Wieczorek
Piotr P
PP
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D011073:Polyamines / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D015203:Reproducibility of Results; D013997:Time Factors; D014105:Tosyl Compounds / Q000737:chemistry*
D008567:Membranes, Artificial; D011073:Polyamines; D014105:Tosyl Compounds; C025506:4-toluenesulfonyl chloride