In vivo calcium imaging of neural network function.
Spatiotemporal activity patterns in local neural networks are fundamental to brain function. Network activity can now be measured in vivo using two-photon imaging of cell populations that are labeled with fluorescent calcium indicators. In this review, we discuss basic aspects of in vivo calcium imaging and highlight recent developments that will help to uncover operating principles of neural circuits.
Göbel
Werner
W
Department of Neurophysiology, Brain Research Institute, University of Zurich, Zurich, Switzerland. goebel@hifo.uzh.ch
Helmchen
Fritjof
F
D000200:Action Potentials; D000818:Animals; D016477:Artifacts; D020013:Calcium Signaling; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D021621:Imaging, Three-Dimensional; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008959:Models, Neurological; D009415:Nerve Net / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D019581:Neuropil / Q000737:chemistry; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted; D013997:Time Factors
D005456:Fluorescent Dyes