title: [Micro-CT evaluation and histological analysis of screw-bone interface of expansile pedicle screw in osteoporotic sheep].
abstract: OBJECTIVE
  To investigate the properties of screw-bone interface of expansive pedicle screw (EPS) in osteoporotic sheep using micro-CT and histology.
  
  
  METHODS
  Six female sheep with bilateral ovariectomy-induced osteoporosis were employed in this experiment and were randomly assigned into 2 groups: A and B. After EPS insertion in each femoral condyles, sheep in group A were bred for 3 months, while those in group B 6 months. Femoral condyles with EPS were 3D-imaged and reconstructed by micro-CT. Histology was evaluated thereafter.
  
  
  RESULTS
  The trabecular microstructure was denser at the screw-bone interface than in the distant parts in expansive section, especially within spiral marking. In the non-expansive section, however, there was no significant difference between the interface and the distant parts. The regions of interest (ROI) adjacent to EPS were reconstructed and analyzed by micro-CT using the same thresholds. The 3D-parameters generated, including tissue mineral density (TMD), bone volume fraction (BVF, BV/TV), bone surface/bone volume (BS/BV) ratio, trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp), were significantly better in expansive than in non-expansive sections (P < 0.05). Histologically, newly formed bone trabeculae crawled along the expansive fissures and into the center of EPS. The newly formed bones, as well as the bone at the bone-screw interface, closely contacted the EPS and constructed four compartments.
  
  
  CONCLUSIONS
  Based on micro-CT and histological evaluation, the study suggest that EPS can significantly provide stabilization in osteoporotic cancellous bone.
authors: Institute of Orthopaedics and Traumatology of People's Liberation Army, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China.
authors: Shi-yong
authors: SY
authors: Wan
authors: Wei
authors: W
authors: Lei
authors: Zi-xiang
authors: ZX
authors: Wu
authors: Rong
authors: R
authors: Lü
authors: Jun
authors: J
authors: Wang
authors: Bo
authors: B
authors: Li
authors: Suo-Chao
authors: SC
authors: Fu
authors: Ce
authors: C
authors: Zhan
mesh_terms: D000818:Animals; D015519:Bone Density; D001863:Bone Screws; D004195:Disease Models, Animal; D005260:Female; D005269:Femur / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000601:surgery; D010024:Osteoporosis / Q000209:etiology / Q000473:pathology* / Q000601:surgery; D010052:Ovariectomy / Q000009:adverse effects; D015203:Reproducibility of Results; D012756:Sheep; D014057:Tomography, X-Ray Computed / Q000379:methods*