title: Novel three-dimensional analysis tool for vascular trees indicates complete micro-networks, not single capillaries, as the angiogenic endpoint in mice overexpressing human VEGF(165) in the brain.
abstract: To adequately supply tissues with oxygen and nutrients, the formation of functional vascular networks requires generation of normal, healthy vessels and their arrangement into an effective network architecture. While our knowledge about the development of single vessels significantly increased during the last years, mechanisms responsible for network formation are still poorly understood. This is probably due to the lack of suitable methods for quantification of structural properties of microvascular networks. Previously we showed that cerebral blood flow is not increased in mice exhibiting a 2- to 3-fold higher density of normal and perfused capillaries as a result of transgenic overexpression of the human vascular endothelial growth factor (VEGF(165)). Here we used vascular corrosion casting and hierarchical micro-computed tomography combined with a new network analysis tool to characterize the vascular architecture in gray and white matter of these mice. Our results indicate that VEGF overexpression leads to formation of additional micro-networks connected to higher order vessels rather than insertion of individual capillaries into the existing vessel structure. This implies that the smallest "angiogenic quantum", i.e. the final, stable result of angiogenesis and subsequent remodeling, is not a single microvessel, but a complete micro-network. In conclusion, high-resolution 3D imaging combined with network analysis can substantially improve our understanding of vascular architecture, beneficial for the development of therapeutic angiogenesis as a clinical tool for applications such as wound healing or treatment of ischemic diseases.
authors: Institute for Biomedical Engineering, University and ETH Zürich, Zürich, Switzerland.
authors: Stefan
authors: S
authors: Heinzer
authors: Gisela
authors: G
authors: Kuhn
authors: Thomas
authors: T
authors: Krucker
authors: Eric
authors: E
authors: Meyer
authors: Alexandra
authors: A
authors: Ulmann-Schuler
authors: Marco
authors: M
authors: Stampanoni
authors: Max
authors: M
authors: Gassmann
authors: Hugo H
authors: HH
authors: Marti
authors: Ralph
authors: R
authors: Müller
authors: Johannes
authors: J
authors: Vogel
mesh_terms: D000465:Algorithms; D000818:Animals; D001808:Blood Vessels / Q000473:pathology*; D001921:Brain / Q000473:pathology*; D002196:Capillaries / Q000473:pathology; D002560:Cerebrovascular Circulation / Q000502:physiology*; D006624:Hippocampus / Q000098:blood supply / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008822:Mice, Transgenic; D009389:Neovascularization, Pathologic / Q000235:genetics* / Q000473:pathology*; D011786:Quality Control; D015203:Reproducibility of Results; D014057:Tomography, X-Ray Computed; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D042461:Vascular Endothelial Growth Factor A