Functional analysis of genes encoding putative oxidoreductases in Aspergillus oryzae, which are similar to fungal fructosyl-amino acid oxidase.
We found 11 genes (FAO1-11) encoding putative oxidoreductases in the Aspergillus oryzae genome, which are similar to fungal fructosyl-amino acid oxidases. The cDNAs corresponding to the genes were cloned and expressed in Escherichia coli. rFao2 had fructosyl-amino acid oxidase activity, whereas rFao1 did not show any enzyme activity, even though the deduced amino acid sequence of Fao1 is identical to that of one of the fructosyl-amino acid oxidase isozymes from Aspergillus oryzae. rFao7 and rFao8 showed oxidase activity toward sarcosine, L-pipecolate, and L-proline. rFao10 was active toward only sarcosine, of the substrates tested. The functions of the other proteins were also predicted from a phylogenetic analysis.
Yoshida
Nobuyuki
N
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630-0192, Japan. yoshidan@bs.naist.jp <yoshidan@bs.naist.jp>
Akazawa
Shin-Ichi
S
Karino
Tetsuya
T
Ishida
Hiroki
H
Hata
Yoji
Y
Katsuragi
Tohoru
T
Tani
Yoshiki
Y
Takagi
Hiroshi
H
D000594:Amino Acid Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010802:Phylogeny; D010875:Pipecolic Acids / Q000737:chemistry; D011392:Proline / Q000737:chemistry; D012521:Sarcosine / Q000737:chemistry; D020539:Sequence Analysis, Protein; D013379:Substrate Specificity
D018076:DNA, Complementary; D005656:Fungal Proteins; D010875:Pipecolic Acids; D011392:Proline; D000594:Amino Acid Oxidoreductases; C103012:amadoriase; C031345:pipecolic acid; D012521:Sarcosine