title: Proliferating and differentiating effects of three different growth factors on pluripotent mesenchymal cells and osteoblast like cells.
abstract: Growth factors are in clinical use to stimulate bone growth and regeneration. BMP-2 is used in long bone and spinal surgery, PDGFbb for the treatment of periodontal defects and children with growth hormone receptor deficiency are treated with IGF-I.Aim of the present study was the comparative analysis of the effect of these growth factors released from a local drug delivery system on cells of the osteogenic lineage at differing differentiation stages.The experiments with the mesenchymal cell line C2C12 revealed a proliferating effect of all three growth factors and a differentiating effect of BMP-2 with a dramatic increase in alkaline phosphatase activity. None of the growth factors stimulated cell migration.Human osteoblast like cells showed similar results with an increase in proliferation after stimulation with IGF-I or PDGFbb. The enzymatic activity of alkaline phosphatase was enhanced only in the cells stimulated with BMP-2. This group showed also more mineralized matrix compared to the other groups.In conclusion, the growth factors IGF-I and PDGFbb delivered with a local drug delivery system stimulated cell proliferation, whereas BMP-2 showed a dramatic effect on differentiation on osteoblast precursor cells and osteoblast like cells.
authors: Center for Musculoskeletal Surgery, Charité-Universitätsmedizin Berlin, Germany. britt.wildemann@charite.de
authors: Britt
authors: B
authors: Wildemann
authors: Nicole
authors: N
authors: Burkhardt
authors: Marc
authors: M
authors: Luebberstedt
authors: Thomas
authors: T
authors: Vordemvenne
authors: Gerhard
authors: G
authors: Schmidmaier