title: Interactions between stainless steel, shear stress, and monocytes.
abstract: Angioplasty with stent placement is commonly used to treat coronary atherosclerosis. However, 20-40% of stainless steel stents restenose within 6 months via a prolonged inflammatory response mediated by monocytic infiltration and cytokine secretion. In the current study, we tested a hypothesis that blood flow and monocytes interact to alter stent corrosion. We assessed the effects of THP1 monocytes on the corrosion rate of 316L stainless steel (316LSS) under shear stress (0.5-50 dyn/cm(2)). In addition, THP1 cytokine secretion was determined using cytokine arrays and ELISA analyses. Data were compared using ANOVA and Tukey post hoc analysis (alpha = 0.05). Monocytes significantly lowered 316LSS corrosion rates without limiting current density. However, shear stress alone did not alter the corrosion rate of 316LSS. THP1 cells adhered to the 316LSS surface at all flow rates. Exposure to the 316LSS/corrosion test under high fluid flow rates increased (>twofold) the secretion of 7 of the 42 cytokines tested (angeogenin, GRO, I309, interleukin 8, interleukin 6, interleukin 1beta, and macrophage chemoattractant protein-1). Each of these cytokines play a role in wound healing, macrophage differentiation, and cell proliferation, all hallmarks of in-stent restenosis. Furthermore, only IL8 levels were significantly higher than any of the system controls during the 316LSS/corrosion test conditions. The IL8 levels from the 316LSS/corrosion tests were not significantly different from the +LPS control. Together, these data suggest that monocytic cells maybe activated by exposure to 316LSS stents and could contribute to in-stent restenosis and altered corrosion of the stent.
authors: Department of Oral Biology and Maxillofacial Pathology, Medical College of Georgia, Augusta, Georgia, USA. rmesser@mail.mcg.edu
authors: Regina L W
authors: RL
authors: Messer
authors: John
authors: J
authors: Mickalonis
authors: Jill B
authors: JB
authors: Lewis
authors: Yo
authors: Y
authors: Omata
authors: Cortney M
authors: CM
authors: Davis
authors: Yolanda
authors: Y
authors: Brown
authors: John C
authors: JC
authors: Wataha
mesh_terms: D000704:Analysis of Variance; D015906:Angioplasty, Balloon, Coronary; D001672:Biocompatible Materials / Q000737:chemistry; D001783:Blood Flow Velocity; D002460:Cell Line; D003324:Coronary Artery Disease / Q000503:physiopathology / Q000628:therapy; D023903:Coronary Restenosis / Q000276:immunology; D003343:Corrosion; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D004563:Electrochemistry; D006801:Humans; D016209:Interleukin-8 / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D008422:Materials Testing; D009000:Monocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D033081:Shear Strength; D013193:Stainless Steel / Q000737:chemistry*; D015607:Stents; D013314:Stress, Mechanical
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D016209:Interleukin-8; D008070:Lipopolysaccharides; D013193:Stainless Steel