title: Synthesis of Leishmania cap-4 intermediates, cap-2 and cap-3.
abstract: Synthesis of Leishmania mRNA 5'-cap analogs, m(7)Gpppm(2)(6)AmpAm (cap-2), and m(7)Gpppm(2)(6)AmpAmpCm (cap-3) is reported. Binding affinities of those cap analogs for LeishIF4E proteins were determined using fluorescence spectroscopy. Cap-3 showed similar affinity to LeishIF4Es compared to the mature trypanosomatids cap structure (cap-4).
authors: Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warsaw, Poland.
authors: Magdalena
authors: M
authors: Lewdorowicz
authors: Janusz
authors: J
authors: Stepinski
authors: Ryszard
authors: R
authors: Kierzek
authors: Jacek
authors: J
authors: Jemielity
authors: Joanna
authors: J
authors: Zuberek
authors: Yael
authors: Y
authors: Yoffe
authors: Michal
authors: M
authors: Shapira
authors: Ryszard
authors: R
authors: Stolarski
authors: Edward
authors: E
authors: Darzynkiewicz
mesh_terms: D000818:Animals; D039561:Eukaryotic Initiation Factor-4E / Q000737:chemistry; D006158:Guanosine Pentaphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry; D007891:Leishmania / Q000378:metabolism*; D012314:RNA Cap Analogs / Q000138:chemical synthesis* / Q000737:chemistry; D012315:RNA Caps / Q000138:chemical synthesis* / Q000737:chemistry; D016053:RNA, Protozoan / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D039561:Eukaryotic Initiation Factor-4E; D012314:RNA Cap Analogs; D012315:RNA Caps; D016053:RNA, Protozoan; D006158:Guanosine Pentaphosphate