High-performance liquid chromatographic evaluation of a coated cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase having a small-pore silica support.
A two-step coating/precipitation synthetic procedure has been developed for the preparation of cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase (CSP) having a small-pore silica support. With this synthetic strategy, monodisperse, spherical CSP particles can be produced without the need for a wasteful and time-consuming sieving process. The performance of the synthesized CSP towards a variety of racemates was evaluated in the normal-phase HPLC mode. HPLC separation experiments revealed that the synthesized CSP exhibited a chiral recognition ability fully comparable to the corresponding commercial columns prepared using conventional large-pore silica as the support. Moreover, the synthesized CSP was successfully applied to semipreparative enantioseparation of a new triazole antifungal agent.
Liu
Yueqi
Y
National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. yqliu2008@yahoo.com
Zou
Hanfa
H
D002482:Cellulose; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D048448:Phenylcarbamates; D011743:Pyrimidines / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D012822:Silicon Dioxide; D014230:Triazoles / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D065819:Voriconazole
D004364:Pharmaceutical Preparations; D048448:Phenylcarbamates; D011743:Pyrimidines; D014230:Triazoles; D012822:Silicon Dioxide; D002482:Cellulose; D065819:Voriconazole