Design and characterization of mechanism-based inhibitors for the tyrosine aminomutase SgTAM.
The synthesis and evaluation of two classes of inhibitors for SgTAM, a 4-methylideneimidazole-5-one (MIO) containing tyrosine aminomutase, are described. A mechanism-based strategy was used to design analogs that mimic the substrate or product of the reaction and form covalent interactions with the enzyme through the MIO prosthetic group. The analogs were characterized by measuring inhibition constants and X-ray crystallographic structural analysis of the co-complexes bound to the aminomutase, SgTAM.
Montavon
Timothy J
TJ
Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, MA 02467, USA.
Christianson
Carl V
CV
Festin
Grace M
GM
Shen
Ben
B
Bruner
Steven D
SD
D018360:Crystallography, X-Ray; D015195:Drug Design; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry* / Q000494:pharmacology; D004852:Epoxy Compounds / Q000737:chemistry* / Q000494:pharmacology; D007093:Imidazoles / Q000737:chemistry; D019751:Intramolecular Transferases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000187:drug effects; D015394:Molecular Structure
C479280:4-methylideneimidazole-5-one; D004791:Enzyme Inhibitors; D004852:Epoxy Compounds; D007093:Imidazoles; D019751:Intramolecular Transferases; C023206:tyrosine 2,3-aminomutase