title: Two novel oligosaccharides isolated from a beverage produced by fermentation of a plant extract.
abstract: An extract from 50 kinds of fruits and vegetables was fermented to produce a new beverage. Natural fermentation of the extract was carried out mainly by lactic acid bacteria (Leuconostoc spp.) and yeast (Zygosaccharomyces spp. and Pichia spp.). Two new saccharides were found in this fermented beverage. The saccharides were isolated using carbon-Celite column chromatography and preparative high performance liquid chromatography. Gas liquid chromatography analysis of methylated derivatives as well as MALDI-TOF MS and NMR measurements were used for structural confirmation. The (1)H and (13)C NMR signals of each saccharide were assigned using 2D-NMR including COSY, HSQC, HSQC-TOCSY, CH(2)-HSQC-TOCSY, and CT-HMBC experiments. The saccharides were identified as beta-D-fructopyranosyl-(2-->6)-beta-D-glucopyranosyl-(1-->3)-D-glucopyranose and beta-D-fructopyranosyl-(2-->6)-[beta-D-glucopyranosyl-(1-->3)]-D-glucopyranose.
authors: Ohtakakohso, Co. Ltd, Otaru 047-0193, Japan.
authors: Naoki
authors: N
authors: Kawazoe
authors: Hideki
authors: H
authors: Okada
authors: Eri
authors: E
authors: Fukushi
authors: Akira
authors: A
authors: Yamamori
authors: Shuichi
authors: S
authors: Onodera
authors: Jun
authors: J
authors: Kawabata
authors: Norio
authors: N
authors: Shiomi
mesh_terms: D001628:Beverages; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002845:Chromatography; D005285:Fermentation; D007934:Leuconostoc / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D020068:Zygosaccharomyces / Q000378:metabolism
chemical_list: D009844:Oligosaccharides; D010936:Plant Extracts