[Association between myocardial ADAMTS-1 expression and myocardial fibrosis in a murine model of viral myocarditis].
OBJECTIVE
To investigate the association between myocardial ADAMTS-1 expression and myocardial fibrosis in coxsackievirus B(3) (CVB(3))-induced acute and chronic murine myocarditis model.


METHODS
Balb/c mice were infected with CVB(3) (single injection or monthly injection for 3 months) to establish acute or chronic myocarditis model. Normal controls received equal-volume Eagles minimal essential medium (EMEM) without CVB(3). Hearts were examined at 7 days or 3 months post CVB(3) infection. Heart slides were stained with collagen specific picrosirius red staining and the collagen volume fraction (CVF) was calculated with image analysis software. The expressions of ADAMTS-1 were determined by RT-PCR and immunohistochemistry.


RESULTS
Compared with controls, the CVF levels and myocardial expressions of ADAMTS-1 were significant increased in two myocarditis groups, especially in mice with chronic myocarditis (P < 0.01). The increased expression of ADAMTS-1 was located in endochylema as visualized by immunohistochemistry. Myocardial ADAMTS-1 mRNA was positively correlated with CVF in both myocarditis groups (r(7 days) = 0.65, P < 0.05; r(3 months) = 0.73, P < 0.01).


CONCLUSIONS
ADAMTS-1 increased in proportion with collagen accumulation in acute and chronic myocarditis, which might play an important role in the development of myocardial fibrosis by modulating the collagen metabolism.
Shen
E
E
Key Laboratory of Viral Heart Diseases, Ministry of Public Health, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Chen
Rui-zhen
RZ
Yang
Ying-zhen
YZ
Guo
Qi
Q
Yu
Yong
Y
Zou
Yun-zeng
YZ
Chen
Hao-zhu
HZ
D051722:ADAM Proteins / Q000378:metabolism*; D000071097:ADAMTS1 Protein; D000208:Acute Disease; D000818:Animals; D002908:Chronic Disease; D003384:Coxsackievirus Infections / Q000473:pathology*; D004195:Disease Models, Animal; D004770:Enterovirus; D005355:Fibrosis / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000473:pathology* / Q000821:virology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology
D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; C486323:Adamts1 protein, mouse