title: A live bovine herpesvirus-1 marker vaccine is not shed after intramuscular vaccination.
abstract: It should be established, whether animals vaccinated intramuscularly (IM) with a live Bovine herpesvirus type 1 (BHV-1) marker vaccine become viremic and/or excrete vaccine virus with nasal discharge. Five cattle, seronegative for BHV-1, were vaccinated with an overdose of the vaccine (Bovilis IBR marker live) via the IM route. Nasal swabs and blood samples were taken at regular intervals and tested for BHV-1 in a virus infectivity assay. In addition, a polymerase chain reaction (PCR) specific for BHV-1 DNA was performed on the blood samples. BHV-1 neutralizing antibody titres were determined in the sera taken prior to the vaccination and four weeks after immunisation. AIl animals were successfully vaccinated as judged by the development of BHV-1 neutralising antibodies. However, all nasal swab samples were tested negative for vaccine virus, and all blood samples were found negative for BHV-1 vaccine virus and BHV-1 specific DNA. From these data it can be concluded that the vaccine virus was not excreted with nasal discharge after IM vaccination and that the vaccinated animals did not have a detectable viremia. Therefore, it is recommended to apply the tested BHV-1 marker live vaccine by the IM route in situations where it is undesirable that the vaccine virus is excreted.
authors: Intervet International b.v.,Wim de Körverstraat 35, NL-5831 AN, Boxmeer, The Netherlands.
authors: Birgit
authors: B
authors: Makoschey
authors: Martin
authors: M
authors: Beer
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control* / Q000821:virology; D004279:DNA, Viral / Q000302:isolation & purification; D006566:Herpesviridae Infections / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D007273:Injections, Intramuscular / Q000662:veterinary; D009666:Nose / Q000821:virology; D014613:Vaccines, Attenuated; D022581:Vaccines, Marker; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D014766:Viremia / Q000662:veterinary; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D014613:Vaccines, Attenuated; D022581:Vaccines, Marker; D014765:Viral Vaccines