title: [The expression of estrogen receptors in the human periodontal ligament fibroblasts in vitro].
abstract: OBJECTIVE
  To explore the expression of estrogen receptors in the human periodontal ligament fibroblasts in vitro.
  
  
  METHODS
  Human periodontal ligament fibroblasts were cultured in vitro. The estrogen receptors (ER)-alpha, ER-beta were detected with immunocytochemistry staining; The mRNA of ER-alpha, ER-beta were measured by reverse transcription polymerase chain reaction; The expression of ER-alpha, ER-beta protein were detected by Western blot.
  
  
  RESULTS
  The mRNA and protein expression of two subtype of ER were observed on human periodontal ligament fibroblasts (HPLF). The intensity of the ER-beta bands were stronger than those of ER-alpha, indicating that ER-beta expression in HPLF was higher.
  
  
  CONCLUSIONS
  ER may play an important role in the function of human periodontal ligament fibroblasts.
authors: Department of Orthodontics, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
authors: Meng
authors: M
authors: Cao
authors: Yin
authors: Y
authors: Ding
authors: Lei
authors: L
authors: Shu
authors: Jia
authors: J
authors: Li
authors: Wei
authors: W
authors: Zhang
authors: Tao
authors: T
authors: Guo
mesh_terms: D000293:Adolescent; D015153:Blotting, Western; D002478:Cells, Cultured; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D010513:Periodontal Ligament / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012333:RNA, Messenger