title: [Studies on the HPLC fingerprint spectrum of flavonoids in Gynostemma pentaphyllum].
abstract: OBJECTIVE
  To establish the HPLC fingerprint spectrum of flavonoids from Gynostemma pentaphyllum.
  
  
  METHODS
  The HPLC conditions with TURNER Kromasil C18 column (250 x 4. 6 mm, 5 microm) was used, the mobile phase consisted of 0.4% phosphoric acid-methanol-acetonitrile with gradient elution at flow rate of 0.5 ml/min and the detection wavelength was set at 360nm.
  
  
  RESULTS
  Ten common characteristic peaks were taken as fingerprint peaks the presision and the retest are in accordance with the requirement.
  
  
  CONCLUSIONS
  The method is stable, reliable, precision and provides a scientific basis for the quality standard of Gynostemma pentaphyllum.
authors: Hubei College of Traditional Chinese Medicine, Wuhan 430061, China. ljq59169@sohu.com
authors: Jin-qing
authors: JQ
authors: Lu
authors: Li
authors: L
authors: Chen
authors: Bo
authors: B
authors: Xiao
authors: Wei
authors: W
authors: Wan
authors: Fan
authors: F
authors: Yu
authors: Yao-ping
authors: YP
authors: Tian
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D029774:Gynostemma / Q000737:chemistry*; D000432:Methanol; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D000432:Methanol