title: Myocardin expression during avian embryonic heart development requires the endoderm but is independent of BMP signaling.
abstract: Myocardin, a serum response factor cofactor, plays an important role in regulating heart and smooth muscle development. To investigate myocardin function during early stages of heart development, we isolated the chicken orthologue of myocardin and characterized its expression between Hamburger and Hamilton stages 3 and 15. At stage 4, myocardin transcripts are detected in the lateral and extraembryonic mesoderm, become progressively localized to the precardiac mesoderm and the differentiated myocardium and are also seen in smooth muscle cells of the developing vascular plexus. Surprisingly, myocardin expression within the developing chicken embryo precedes that of the homeodomain transcription factor Nkx2.5. Embryonic dissection studies demonstrate that signals from the endoderm are required for myocardin expression within the precardiac mesoderm. However, unlike Nkx2.5, myocardin expression is not regulated by bone morphogenetic protein (BMP) signaling. These results suggest that initial expression of myocardin in the precardiac mesoderm is regulated by a signaling pathway that is parallel to, and independent of, Nkx2.5 expression.
authors: Department of Cell Biology and Anatomy, University of Arizona, Tucson, Arizona, USA.
authors: Andrew S
authors: AS
authors: Warkman
authors: Tatiana A
authors: TA
authors: Yatskievych
authors: Katharine M
authors: KM
authors: Hardy
authors: Paul A
authors: PA
authors: Krieg
authors: Parker B
authors: PB
authors: Antin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000502:physiology*; D016466:CHO Cells; D002352:Carrier Proteins / Q000235:genetics; D002642:Chick Embryo; D006224:Cricetinae; D003412:Cricetulus; D004707:Endoderm / Q000196:embryology / Q000378:metabolism*; D006321:Heart / Q000196:embryology*; D017403:In Situ Hybridization; D008648:Mesoderm / Q000196:embryology / Q000378:metabolism; D008969:Molecular Sequence Data; D024510:Muscle Development / Q000235:genetics / Q000502:physiology; D009687:Nuclear Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D000099176:Noggin Protein; D000099251:Myocardin
chemical_list: D019485:Bone Morphogenetic Proteins; D002352:Carrier Proteins; D009687:Nuclear Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D000099176:Noggin Protein; D000099251:Myocardin