Expression of mRNA for functional molecules in urinary sediment in glomerulonephritis.
Recent studies have suggested that gene expression studies using urinary sediment might be a non-invasive approach to assessing activity and pathogenesis in glomerulonephritis. However, little information is available regarding the mRNA expression patterns of functional molecules, such as T-bet, GATA-3, FOXP3, and retinoic acid-inducible gene-I (RIG-I), in urinary sediment, from patients with immunocomplex-mediated glomerulonephritis. Fourteen lupus nephritis (LN) patients, 13 IgA nephropathy (IgAN) patients, and 12 healthy controls were enrolled in the study. The mRNA expressions of T-bet, GATA-3, FOXP3 and RIG-I in urinary sediment were measured using real time quantitative polymerase chain reaction. We also studied the expression of RIG-I in kidney tissue specimens obtained from LN and IgAN patients. Significant differences in the expression patterns of GATA-3, FOXP3 and RIG-I, and marginal differences in T-bet expression, were observed between the three study groups. Immunofluorescent staining for RIG-I was observed in the tissue specimens from the LN patients, but not in those from the IgAN patients. The mRNA expression patterns of T-bet, GATA-3, FOXP3 and RIG-I in urinary sediment differ according to diagnostic category. These results suggest that the measurement of these target gene expressions might be a useful, non-invasive method for clinical monitoring and studying of pathogenesis in glomerulonephritis.
Tsugawa
Koji
K
Department of Pediatrics, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan. kojituga@royal.ocn.ne.jp
Oki
Eishin
E
Suzuki
Koichi
K
Imaizumi
Tadaatsu
T
Ito
Etsuro
E
Tanaka
Hiroshi
H
D000293:Adolescent; D000328:Adult; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000235:genetics; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics; D050990:GATA3 Transcription Factor / Q000235:genetics; D005921:Glomerulonephritis / Q000378:metabolism*; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000652:urine*; D011971:Receptors, Immunologic; D020825:T-Box Domain Proteins / Q000235:genetics; D000098815:T-bet Transcription Factor
C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases