title: Antimicrobial effects of Piper guineense 'Uziza' and Phyllantus amarus 'Ebe-benizo' on Candida albicans and Streptococcus faecalis.
abstract: Two pathogens were employed, Streptococcus faecalis and Candida albicans for the study of the antimicrobial effects of Piper guineense and Phyllantus amarus using agar-well diffusion and disc-diffusion methods. Phytochemical screening of ethanol, cold and hot water extracts detected the presence of tannins, saponins, flavonoids, terpenoids and phlobatannins. Antimicrobial effect of the plant extracts showed that the organic solvent and aqueous solvents of P. amarus were inhibitory to S. faecalis while the extracts of Phyllantus amarus were not inhibitory to Candida albicans. Agar-well determined Minimum Inhibitory Concentration (MIC) values ranged between 3.125 mg/ml and 6.25 mg/ml while the disc diffusion determined MIC values ranged between 6.25 mg/ml and 25.0 mg/ml. The agar-well determined MIC values for the ethanolic P. amarus extracts (3.12 mg/ml) were lower than the corresponding disc-diffusion MIC determined values (6.25 mg/ml-25.00 mg/ml). Bacteriocidal and bacteriostatic effect varied with, solvent type of extract, concentration of the plant extract and the method of the test adopted. The active components of the plant have no antifungal effect on the tested yeast (Candida albicans). These findings are discussed in relation to plant chemicals as a means of disease control and also to the problem of microbial resistance to synthetic drugs.
authors: Department of Botany, Nnamdi Azikiwe University, PMB 5025, Awka, Anambra State, Nigeria. okigborn17@yahoo.com
authors: R N
authors: RN
authors: Okigbo
authors: D I
authors: DI
authors: Igwe
mesh_terms: D002176:Candida albicans / Q000187:drug effects*; D013293:Enterococcus faecalis / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D029803:Phyllanthus; D031701:Piper; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts