title: [Over-expression of hypoxia-inducible factor-1 alpha increases angiogenesis of LNCaP cells].
abstract: OBJECTIVE
  To evaluate the effect of hypoxia-inducible factor-1 alpha (HIF-la) on angiogenesis in human prostate cancer cells.
  
  
  METHODS
  Human prostate cancer cells of the line LNCaP were cultured and transfected by the recombinant plasmid pcDNA3. 1(-)/HIF-1alpha containing the gene HIF-1alpha with the Lipofectamine 2000 system. The positive clone cells were selected by G418 and confirmed by Western blotting and immunofluorescence (LNCaP/HIF-1alpha cells). The expressions of VEGF, iNOS and Ang-2 were detected by Western blotting.
  
  
  RESULTS
  The expression of HIF-1alpha in the LNCaP/HIF-1alpha cells was distinctly higher than that in the LN-CaP cells. Compared with the LNCaP cells, the expressions of VEGF and iNOS were up-regulated, whereas Ang-2 remained unchanged in the LNCaP/HIF-1alpha cells.
  
  
  CONCLUSIONS
  The over-expression of HIF-1alpha can induce an increase in angiogenesis proteins and improve the angiogenesis potency of prostate cancer.
authors: Department of Urology, the First Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
authors: Yi-li
authors: YL
authors: Han
authors: Da-lin
authors: DL
authors: He
authors: Yong
authors: Y
authors: Luo
authors: He-peng
authors: HP
authors: Cheng
authors: Guang-feng
authors: GF
authors: Zhu
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D005455:Fluorescent Antibody Technique; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry; D008297:Male; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D011471:Prostatic Neoplasms / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D014162:Transfection / Q000379:methods; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008055:Lipids; C086724:Lipofectamine; D042461:Vascular Endothelial Growth Factor A; D019001:Nitric Oxide Synthase