Cloning and expression of a parathion hydrolase gene from a soil bacterium, Burkholderia sp. JBA3.
A bacterium, Burkholderia sp. JBA3, which can mineralize the pesticide parathion, was isolated from an agricultural soil. The strain JBA3 hydrolyzed parathion to p-nitrophenol, which was further utilized as the carbon and energy sources. The parathion hydrolase was encoded by a gene on a plasmid that strain JBA3 harbored, and it was cloned into pUC19 as a 3.7-kbp Sau3AI fragment. The ORF2 (ophB) in the cloned fragment encoded the parathion hydrolase composed of 526 amino acids, which was expressed in E. coli DH10B. The ophB gene showed no significant sequence similarity to most of other reported parathion hydrolase genes.
Kim
Taesung
T
Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
Ahn
Jae-Hyung
JH
Choi
Min-Kyeong
MK
Weon
Hang-Yeon
HY
Kim
Mi Sun
MS
Seong
Chi Nam
CN
Song
Hong-Gyu
HG
Ka
Jong-Ok
JO
D019117:Burkholderia / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D006867:Hydrolases / Q000235:genetics*; D010278:Parathion / Q000378:metabolism*; D012988:Soil Microbiology
D010278:Parathion; D006867:Hydrolases