Pharmacokinetics of linezolid in bone tissue investigated by in vivo microdialysis.
Pharmacokinetics of unbound anti-infectives in bone is difficult to characterize. The aim of this study was to assess the feasibility of the microdialysis technique to cancellous bone for single dose pharmacokinetic investigations of the anti-infective linezolid. Serial bone biopsies (left tibia) and microdialysate samples (right tibia: 2 catheters) as well as plasma and bone marrow samples were obtained from 10 pigs. The concentrations of linezolid reached bacteriostatic levels in plasma, bone marrow, bone biopsies and microdialysates. With the use of microdialysis we here present the first results for unbound linezolid bone penetration. Unbound linezolid concentrations in bone obtained by microdialysis were lower than might have been expected from previous bone biopsy studies. To achieve effective concentrations (24 h) for susceptible organisms the chosen dose of linezolid might not be sufficient.
D000081:Acetamides / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001842:Bone and Bones / Q000737:chemistry; D005240:Feasibility Studies; D005260:Female; D007262:Infusions, Intravenous; D000069349:Linezolid; D017551:Microdialysis / Q000379:methods; D023303:Oxazolidinones / Q000008:administration & dosage / Q000493:pharmacokinetics*; D034421:Sus scrofa; D014018:Tissue Distribution
D000081:Acetamides; D000890:Anti-Infective Agents; D023303:Oxazolidinones; D000069349:Linezolid