A facile method to construct hybrid multilayered films as a strong and multifunctional antibacterial coating.
A multifunctional multilayered film containing TiO(2) nanoparticles as contact-active antibacterial agent and nanosilver as a release-active antibacterial agent was fabricated via layer-by-layer assembly. TiO(2) nanoparticles with the anatase crystalline dominant structure were synthesized via a sol-gel method. The QCM, AFM, and contact angle measurement results indicated that the TiO(2) nanoparticle-chitosan was successfully assembled with heparin via layer-by-layer assembly. The UV visible spectroscopy demonstrated that the silver ions could be loaded into the multilayers and in situ synthesize silver nanoparticles in the multilayers template. The short-term antibacterial assay showed the TiO(2)-chitosan/heparin multilayers loaded with nanosilver was bactericidal both in the low intensity UV light and in the dark. The long-term antibacterial assay indicated although the antibacterial in dark decreased with the PBS immersion time, the hybrid multilayered films sustained the long-term antibacterial in the low intensity UV light.
D000900:Anti-Bacterial Agents / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry; D004926:Escherichia coli / Q000254:growth & development*; D008567:Membranes, Artificial; D008826:Microbial Sensitivity Tests / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D010777:Photochemistry; D017319:Photosensitizing Agents / Q000737:chemistry*; D012834:Silver / Q000737:chemistry; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*; D014466:Ultraviolet Rays
D000900:Anti-Bacterial Agents; D008567:Membranes, Artificial; D017319:Photosensitizing Agents; C009495:titanium dioxide; D012834:Silver; D048271:Chitosan; D014025:Titanium