Anterograde transsynaptic tracing in the murine somatosensory system using Pseudorabies virus (PrV): a "live-cell"-tracing tool for analysis of identified neurons in vitro.
The Pseudorabies virus (PrV) strain Bartha is widely used as a tool for retrograde transneuronal tracing in mammals. Traced neurons can be identified in cell culture allowing the analysis of their physiological features ("live-cell"-tracing). Compared to PrV-Bartha, PrV-Kaplan is known for higher virulence and transsynaptic spread in both retrograde and anterograde direction. Herein we assess the authors assess PrV-Kaplan for transsynaptic anterograde "live-cell"-tracing. Following intranasal application in mice, labelled trigeminal and brainstem neurons could be identified in vitro. Detailed electrophysiological analysis indicated that viral infection did not affect neuronal properties, making PrV-Kaplan eligible for functional analysis of identified neurons within somatosensory systems.
D000818:Animals; D002490:Central Nervous System / Q000502:physiology / Q000821:virology*; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000821:virology*; D011557:Pseudorabies / Q000473:pathology / Q000821:virology*; D013569:Synapses / Q000502:physiology / Q000821:virology*; D009435:Synaptic Transmission