Genetic relatedness of noroviruses identified in sporadic gastroenteritis in children and gastroenteritis outbreaks in northern Alberta.
We compared the proportion and genotype distribution of norovirus (NoV) identified in sporadic acute gastroenteritis in children younger than 7 years old with the NoV strains found in outbreaks from January 2003 through April 2004 in northern Alberta, Canada. Eight genogroup I (GI) and 133 GII NoV cases were detected in 1,166 cases of acute sporadic childhood gastroenteritis with a monthly detection rates varying from 6.0% to 20.4% and no sporadic gastroenteritis case in October 2003. Seventy-eight outbreaks (65%) tested positive for NoV during the study period with an obvious winter predominance and no NoV outbreaks in August, September, and October 2003. Three GI and 51 GII strains from the sporadic childhood gastroenteritis cases and seven GI and 37 GII strains from gastroenteritis outbreaks were sequenced and analyzed. Strains belonging to the GII.4 cluster predominated in outbreaks (68%) while the strains in sporadic childhood gastroenteritis demonstrated significant heterogeneity with the majority belonging to the GII.3 cluster (36%). Further analysis of NoV strains from 34 sporadic childhood gastroenteritis cases and 38 gastroenteritis outbreaks in chronologically and geographically related groups failed to demonstrate clear link between strains circulating in the setting of sporadic childhood gastroenteritis and those found in outbreaks.
D000416:Alberta / Q000453:epidemiology; D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D004196:Disease Outbreaks; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D006801:Humans; D007223:Infant; D017720:Molecular Epidemiology; D029322:Norovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D011110:Polymorphism, Genetic; D012367:RNA, Viral / Q000235:genetics; D012621:Seasons; D017422:Sequence Analysis, DNA
D012367:RNA, Viral