title: Regulation of the murine TR2/HVEM gene expression by IRF.
abstract: TR2 (TNFR-related 2, HVEM, or TNFRSF-14), a member of the TNFR family, is involved in a number of immune responses. While TR2 is expressed on the surface of T cells during the resting state, little is known regarding how expression of the TR2 gene is regulated. To understand the mechanisms regulating the expression of TR2 in T cells, we analyzed the 5' flanking region of TR2. We identified an important region for the activity of the TR2 promoter using site directed mutagenesis. Using EMSA analysis, we found that IRF-2 was bound to the promoter region of the TR2 gene during the resting state of EL-4 T cells. Transfection of IRF-2 expression plasmid and of dominant negative IRF-2 mutant further confirmed our results. Together, these data demonstrate that IRF-2 is involved in the regulation of TR2 expression in EL-4 T cells.
authors.affiliation: Department of Biological Science and the Immunomodulation Research Center, University of Ulsan, San29 Mugeo-Dong, Nam-Gu, Ulsan 680-749, Republic of Korea.
authors.forename: Ok-Ju
authors.initials: OJ
authors.lastname: Sul
authors.forename: Hyeon-Woo
authors.initials: HW
authors.lastname: Lee
authors.forename: Woon-Ki
authors.initials: WK
authors.lastname: Kim
authors.forename: Jin-Hee
authors.initials: JH
authors.lastname: Choi
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Kwak
authors.forename: Youn-Jung
authors.initials: YJ
authors.lastname: Choi
authors.forename: Anne M
authors.initials: AM
authors.lastname: Latour
authors.forename: Beverly H
authors.initials: BH
authors.lastname: Koller
authors.forename: Choon-Soo
authors.initials: CS
authors.lastname: Jeong
authors.forename: Byoung S
authors.initials: BS
authors.lastname: Kwon
mesh_terms: D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation / Q000235:genetics*; D050836:Interferon Regulatory Factor-2 / Q000235:genetics; D051379:Mice; D011401:Promoter Regions, Genetic / Q000235:genetics*; D053240:Receptors, Tumor Necrosis Factor, Member 14 / Q000235:genetics / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D050836:Interferon Regulatory Factor-2; C494230:Irf2 protein, mouse; D053240:Receptors, Tumor Necrosis Factor, Member 14; C506029:Tnfrsf14 protein, mouse