title: Modulation of nucleobindin-1 and nucleobindin-2 by caspases.
abstract: Nucleobindin-1 (NUCB1) and nucleobindin-2 (NUCB2) are multifunctional proteins that interact with Ca(2+), nucleic acids and various regulatory proteins in different signaling pathways. So far, our understanding of the regulation of the biological functions of nucleobindins remains limited. In our proteome-wide selection for downstream caspase substrates, both NUCB1 and NUCB2 are found to be the downstream substrates of caspases. We report here the detailed analyses of the cleavage of nucleobindins by caspases. Significantly, the caspase cleavage sites are located exactly at one of the Ca(2+)-binding EF-hand motifs. Our results suggest that the functions of nucleobindins could be modulated by caspase-mediated cleavage in apoptosis.
authors.affiliation: School of Pharmacy and Carolina Center for Genome Sciences, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: C Alexander
authors.initials: CA
authors.lastname: Valencia
authors.forename: Steven W
authors.initials: SW
authors.lastname: Cotten
authors.forename: Jinzhu
authors.initials: J
authors.lastname: Duan
authors.forename: Rihe
authors.initials: R
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020013:Calcium Signaling; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D020169:Caspases / Q000378:metabolism*; D002384:Catalysis; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D000080866:Nucleobindins; D017386:Sequence Homology, Amino Acid
chemical_list: D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; C000630649:NUCB1 protein, human; C000630650:NUCB2 protein, human; D009419:Nerve Tissue Proteins; D000080866:Nucleobindins; D020169:Caspases