title: High-affinity uranyl-specific antibodies suitable for cellular imaging.
abstract: Monoclonal antibodies (mAbs) have proved to be valuable models for the study of protein-metal interactions, and previous reports have described very specific antibodies to chelated metal ions, including uranyl. We raised specific mAbs against UO2(2+)-DCP-BSA (DCP, 1,10-phenanthroline-2,9-dicarboxylic acid) to generate new sets of antibodies that might cross-react with various complexed forms of uranyl in different environments for further application in the field of toxicology. Using counter-screening with UO2(2+)-DCP-casein, we selected two highly specific mAbs against uranyl-DCP ( K D 10-100 pM): U04S and U08S. Competitive assays in the presence of different metal ions (UO2(2+), Fe (3+), Zn2+, Cu2+, and Ca2+) showed that uranyl in solution can act as a good competitor, suggesting some antibody ability to cross-react with chelating groups other than DCP in the UO2(2+) equatorial coordination plane. Interestingly, one of the antibodies could be used for revealing uranyl cations in cell samples. Fluorescence activated cell sorting analyses after immunolabeling revealed the interaction of uranyl with human kidney cells HK2. The intracellular accumulation of uranyl could be directly visualized by metal-immunostaining using fluorescent-labeled mAb. Our results suggest that U04S mAb epitopes mostly include the uranyl fraction and its paratopes can accommodate a wide variety of chelating groups.
authors.affiliation: CEA Valrhô, DSV/IBEB/Service de Biochimie et de Toxicologie Nucléaire, BP 17171, F-30207 Bagnols sur Cèze, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Reisser-Rubrecht
authors.forename: Caroline
authors.initials: C
authors.lastname: Torne-Celer
authors.forename: Wendy
authors.initials: W
authors.lastname: Rénier
authors.forename: Olivier
authors.initials: O
authors.lastname: Averseng
authors.forename: Sophie
authors.initials: S
authors.lastname: Plantevin
authors.forename: Eric
authors.initials: E
authors.lastname: Quéméneur
authors.forename: Laurent
authors.initials: L
authors.lastname: Bellanger
authors.forename: Claude
authors.initials: C
authors.lastname: Vidaud
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D001666:Binding Sites, Antibody; D045744:Cell Line, Tumor; D002614:Chelating Agents / Q000737:chemistry / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D006825:Hybridomas; D007668:Kidney / Q000378:metabolism; D007687:Kidney Tubules, Proximal / Q000166:cytology* / Q000378:metabolism; D017974:Uranium Compounds / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D017974:Uranium Compounds