Highly sensitive assays for SUMOylation and small ubiquitin-like modifier-dependent protein-protein interactions.
Small ubiquitin-like proteins (SUMO) are recently discovered post-translational modifiers that regulate protein functions and intracellular trafficking. In this study, we are describing two chemoluminescence-based assays, one for SUMOylation and another one for SUMO-mediated protein-protein interactions. These assays can be used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation, and in high-throughput screening for inhibitors of SUMOylation and SUMO-dependent protein-protein interactions. These novel assays represent the most sensitive assays for ubiquitin-like systems published to date. Similar strategies can be used to develop assays for other ubiquitin-like modification systems.
D007700:Kinetics; D008163:Luminescent Measurements; D011485:Protein Binding; D012680:Sensitivity and Specificity; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism*
D025841:Small Ubiquitin-Related Modifier Proteins; D025801:Ubiquitin