title: Quantitative structured-illumination phase microscopy.
abstract: We introduce a quantitative phase imaging method for homogeneous objects with a bright field transmission microscope by using an amplitude mask and a digital processing algorithm. A known amplitude pattern is imaged on the sample plane containing a thick phase object by placing an amplitude mask in the field diaphragm of the microscope. The phase object distorts the amplitude pattern according to its optical path length (OPL) profile, and the distorted pattern is recorded in a CCD detector. A digital processing algorithm then estimates the object's quantitative OPL profile based on a closed form analytical solution, which is derived using a ray optics model for objects with small OPL gradients.
authors.affiliation: Micro Optical-Imaging Systems Laboratory, Department of Electrical and Computer Engineering, University of Colorado, Campus Box 425, Boulder, Colorado 80309, USA. pavani@colorado.edu
authors.forename: Sri Rama Prasanna
authors.initials: SR
authors.lastname: Pavani
authors.forename: Ariel R
authors.initials: AR
authors.lastname: Libertun
authors.forename: Sharon V
authors.initials: SV
authors.lastname: King
authors.forename: Carol J
authors.initials: CJ
authors.lastname: Cogswell
mesh_terms: D000465:Algorithms; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008029:Lighting / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation / Q000379:methods*; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted