Diagnosis of Aspergillus fumigatus endophthalmitis from formalin fixed paraffin-embedded tissue by polymerase chain reaction-based restriction fragment length polymorphism.
New molecular biological technique of Polymerase Chain Reaction (PCR) based Restriction Fragment Length Polymorphism (RFLP) can identify the species from paraffin-embedded tissue section. We demonstrated Aspergillus fumigatus fungus by PCR-based RFLP technique from paraffin section of an eyeball of an eight-month-old child removed for endogenous endophthalmitis.
D001228:Aspergillosis / Q000175:diagnosis*; D001232:Aspergillus fumigatus / Q000145:classification* / Q000235:genetics; D009877:Endophthalmitis / Q000175:diagnosis / Q000382:microbiology*; D015821:Eye Infections, Fungal; D006801:Humans; D007223:Infant; D008297:Male; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016707:Tissue Fixation