title: Improved surface modification approach for micromechanical biosensors.
abstract: We have investigated the sensing performance of protein-based microcantilever biosensors prepared from multiple surface conjugation chemistries. The 11-mercaptoundecanoic acid monolayers were prepared according to both traditional and modified processes. In three protein-based biosensors, the modified process improved microcantilever sensing performance by increasing the bending amplitude, a critical step toward developing a cost-effective microcantilever-based sensor platform for medical diagnostics and environmental and drug screening applications. Scanning electron microscopy (SEM) images demonstrated that proteins immobilized on the microcantilever surface using the modified chemistry approach formed a compact layer.
authors.affiliation: Chemistry, Institute for Micromanufacturing, Louisiana Tech University, Ruston, Louisiana 71272, USA.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Gao
authors.forename: Koutilya R
authors.initials: KR
authors.lastname: Buchapudi
authors.forename: Abraham
authors.initials: A
authors.lastname: Harms-Smyth
authors.forename: Marvin K
authors.initials: MK
authors.lastname: Schulte
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Xu
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Ji
mesh_terms: D015374:Biosensing Techniques; D008855:Microscopy, Electron, Scanning; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties