Synthesis of an anthrose derivative and production of polyclonal antibodies for the detection of anthrax spores.
A straightforward synthesis of a derivative of anthrose, the non-reducing terminal fragment of the antigenic tetrasaccharide from Bacillus anthracis, was achieved starting from d-galactose. This hapten is able to induce a highly specific and sensitive immune response in rabbit when attached to a carrier protein.
D000606:Amino Sugars / Q000138:chemical synthesis*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001408:Bacillus anthracis / Q000276:immunology*; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D006433:Hemocyanins / Q000276:immunology; D007124:Immunoenzyme Techniques / Q000379:methods; D011817:Rabbits; D013171:Spores, Bacterial / Q000737:chemistry / Q000276:immunology*
C504007:4,6-dideoxy-4-(3-hydroxy-3-methylbutanamido)-2-O-methylglucopyranose; D000606:Amino Sugars; D000907:Antibodies, Bacterial; D006433:Hemocyanins; D003847:Deoxyglucose; C032808:keyhole-limpet hemocyanin