title: [Detection of enterovirus in cerebrospinal fluid by real-time nested reverse transcription polymerase chain reaction.].
abstract: BACKGROUND
  Enterovirus is a common cause of aseptic meningitis, respiratory disease and nonspecific febrile illness. The conventional methods for laboratory diagnosis of enterovirus infections have been virus culture and serotyping by an immunofluorecent test. We studied a new and more rapid approach for enterovirus detection in cerebrospinal fluid (CSF) by real-time nested PCR.
  
  
  METHODS
  This study was performed on 50 CSF specimens from patients suspected of aseptic meningitis. Enterovirus was detected in CSF by PCRs for 3 different targets and real-time nested PCR. Enterovirus culture was also performed in 44 CSF specimens.
  
  
  RESULTS
  The positive rate of PCRs for each of the 3 different targets was 26.0%, 40.0%, or 46.0%, and that of real-time nested PCR was 86.0%. Only 6.8% were positive in culture. Thus, the positive rate of real-time nested PCR was much higher than other methods.
  
  
  CONCLUSIONS
  Our study revealed that the real-time nested PCR should be useful for diagnosis of enterovirus infections because of a high sensitivity and rapid detection.
authors.affiliation: Department of Laboratory Medicine, Seoul National University Bundang Hospital, Korea. m91w95@dreamwiz.com.
authors.forename: Se Ran
authors.initials: SR
authors.lastname: Heo
authors.forename: Sun Kyung
authors.initials: SK
authors.lastname: Jin
authors.forename: Ho Eun
authors.initials: HE
authors.lastname: Chang
authors.forename: Kyoung Un
authors.initials: KU
authors.lastname: Park
authors.forename: Junghan
authors.initials: J
authors.lastname: Song
authors.forename: Eui Chong
authors.initials: EC
authors.lastname: Kim