title: Autofluorescent proteins.
abstract: Autofluorescent proteins (AFPs) have revolutionized molecular cell biology, and applications continue to harness the power of these genetically encoded fluorescent tags. Here, we review the discovery and physical properties of AFPs as well as their development through mutational optimization for several functional parameters. A practical guide to selection and use of major AFPs is provided as well as an overview of techniques for experimental applications.
authors.affiliation: Department of Biochemistry, National University of Ireland, Galway, Ireland.
authors.forename: Ian M
authors.initials: IM
authors.lastname: Dobbie
authors.forename: Noel F
authors.initials: NF
authors.lastname: Lowndes
authors.forename: Kevin F
authors.initials: KF
authors.lastname: Sullivan
mesh_terms: D000595:Amino Acid Sequence; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism; D008164:Luminescent Proteins / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins