title: Diagnosis of Aspergillus fumigatus endophthalmitis from formalin fixed paraffin-embedded tissue by polymerase chain reaction-based restriction fragment length polymorphism.
abstract: New molecular biological technique of Polymerase Chain Reaction (PCR) based Restriction Fragment Length Polymorphism (RFLP) can identify the species from paraffin-embedded tissue section. We demonstrated Aspergillus fumigatus fungus by PCR-based RFLP technique from paraffin section of an eyeball of an eight-month-old child removed for endogenous endophthalmitis.
authors.affiliation: Medical and Vision Research Foundations, Sankara Nethralaya, Chennai, India. drjb@snmail.org
authors.forename: Jyotirmay
authors.initials: J
authors.lastname: Biwas
authors.forename: R
authors.initials: R
authors.lastname: Bagyalakshmi
authors.forename: Lily K
authors.initials: LK
authors.lastname: Therese
mesh_terms: D001228:Aspergillosis / Q000175:diagnosis*; D001232:Aspergillus fumigatus / Q000145:classification* / Q000235:genetics; D009877:Endophthalmitis / Q000175:diagnosis / Q000382:microbiology*; D015821:Eye Infections, Fungal; D006801:Humans; D007223:Infant; D008297:Male; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016707:Tissue Fixation