Protective effects of naringenin-7-O-glucoside on doxorubicin-induced apoptosis in H9C2 cells.
Doxorubicin, a widely used chemotherapeutic agent, can give rise to severe cardiotoxicity by inducing cardiomyocyte apoptosis. Dracocephalum rupestre Hance, a Chinese traditional herb, has therapeutic potential for cardiovascular diseases. Naringenin-7-O-glucoside is the main active constituent of D. rupestre and there is increasing interest in its therapeutic applications. The aim of this study was to evaluate the effects of naringenin-7-O-glucoside on cardiomyocyte apoptosis induced by doxorubicin. Cell viability was detected by MTT assay. Naringenin-7-O-glucoside (10, 20, and 40 microM) significantly enhanced cardiomyocyte proliferation relative to that of doxorubicin. Furthermore, naringenin-7-O-glucoside increased the protein levels of heme oxygenase-1 (HO-1) and Bcl-2 in cardiomyocytes (as detected by Western blotting) and suppressed the mRNA expression of caspase-3 and caspase-9 (as detected by RT-PCR). These results suggest that naringenin-7-O-glucoside has protective effects against doxorubicin-induced apoptosis, effects which could underlie the use of naringenin-7-O-glucoside therapeutic agent for treating or preventing cardiomyopathy associated with doxorubicin.
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000235:genetics; D053453:Caspase 9 / Q000235:genetics; D002478:Cells, Cultured; D019610:Cytoprotection; D004317:Doxorubicin / Q000633:toxicity*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D044950:Flavanones / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology*; D051547:Heme Oxygenase-1 / Q000032:analysis / Q000235:genetics; D019686:Lamiaceae / Q000737:chemistry*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000235:genetics; D051381:Rats
D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005960:Glucosides; D019253:Proto-Oncogene Proteins c-bcl-2; C529068:naringenin-7-O-glucoside; D004317:Doxorubicin; D051547:Heme Oxygenase-1; D053148:Caspase 3; D053453:Caspase 9