title: Detection of Verotoxigenic Escherichia coli O157 and O26 in food by plating methods and LAMP method: a collaborative study.
abstract: In order to establish a rapid and sensitive method for the detection of Verotoxigenic Escherichia coli O157 and O26, a collaborative study was conducted focusing on a comparison of the efficiency of loop-mediated amplification (LAMP) assay targeting the Verocytotoxin (also called Shiga toxin) gene, utilizing a direct plating method and a plating method with immunomagnetic separation (IMS-plating method) using various agar media. In combination with enrichment with the modified EC supplemented with novobiocin, E. coli O157 was detected in most samples of ground beef and alfalfa sprouts by LAMP assay, the direct plating method and the IMS-plating method. E. coli O26 was detected in approximately 100% of the food samples by LAMP assay. However, the IMS-plating and direct plating methods recovered 80 and 50% in ground beef samples, respectively. As a result, it was demonstrated the LAMP assay is superior to the IMS-plating method. Based on these results, it appears LAMP assay is effective as a screening assay to detect E. coli O157 and O26 from positive samples.
authors.affiliation: Division of Microbiology, National Institute of Health Sciences, Setagaya-ku, 1-18-1 Kamiyoga, Tokyo 158-8501, Japan. ykudo@nihs.go.jp
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Hara-Kudo
authors.forename: Noriko
authors.initials: N
authors.lastname: Konishi
authors.forename: Kayoko
authors.initials: K
authors.lastname: Ohtsuka
authors.forename: Reiji
authors.initials: R
authors.lastname: Hiramatsu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Konuma
authors.forename: Kosuke
authors.initials: K
authors.lastname: Takatori
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D008461:Meat Products / Q000382:microbiology; D000455:Medicago sativa / Q000382:microbiology; D012680:Sensitivity and Specificity; D054323:Shiga-Toxigenic Escherichia coli / Q000302:isolation & purification*
chemical_list: D003470:Culture Media