title: Noradrenaline release by activation of κ-bungarotoxin-sensitive nicotinic acetylcholine receptors participates in long-term potentiation-like response induced by nicotine.
abstract: Nicotine enhances the function of learning and memory, but the underlying mechanism still remains unclear. Hippocampal long-term potentiation (LTP) is assumed to be a cellular mechanism of learning and memory. Our previous experiments showed that with the single pulses evoking 80% of the maximal population spike (PS) amplitude, nicotine (10 μmol/L) induced LTP-like response in the hippocampal CA1 region. In the present study, the nicotinic acetylcholine receptor (nAChR) subtypes and relevant neurotransmitter releases involved in LTP-like response induced by nicotine were investigated by extracellularly recording the PS in the pyramidal cell layer in the hippocampal CA1 region in vitro. LTP-like response induced by nicotine was blocked by mecamylamine (1 μmol/L) or κ-bungarotoxin (0.1 μmol/L), but not by dihydro-β-erythtroidine (DHβE, 10 μmol/L). Moreover, it was inhibited by propranolol (10 μmol/L), but not by phentolamine (10 μmol/L) or atropine (10 μmol/L). The results suggest that noradrenaline release secondary to the activation of κ-bungarotoxin-sensitive nAChRs participates in LTP-like response induced by nicotine in the hippocampal CA1 region.
authors.affiliation: Experimental Teaching Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Yu
authors.forename: Jin
authors.initials: J
authors.lastname: He
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Deng
authors.forename: Xiao-Nan
authors.initials: XN
authors.lastname: Zhu
authors.forename: Xue-Lan
authors.initials: XL
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Ru-Zhu
authors.initials: RZ
authors.lastname: Chen
mesh_terms: D000818:Animals; D002038:Bungarotoxins; D056547:CA1 Region, Hippocampal / Q000502:physiology; D017774:Long-Term Potentiation / Q000187:drug effects*; D009538:Nicotine / Q000494:pharmacology*; D009638:Norepinephrine / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000378:metabolism*
chemical_list: D002038:Bungarotoxins; D011978:Receptors, Nicotinic; D009538:Nicotine; D009638:Norepinephrine