title: Optimizing ssNMR experiments for dilute proteins in heterogeneous mixtures at high magnetic fields.
abstract: Solid-state NMR spectroscopy at high magnetic fields is proving to be an effective technique in structural biology, particularly for proteins which are not amenable to traditional X-ray and solution NMR approaches. Several parameters can be selected to provide optimal sensitivity, improve sample stability, and ensure biological relevance for ssNMR measurements on protein samples. These include selection of sample conditions, NMR probe design, and design of pulse experiments. Here, we demonstrate and evaluate several engineering and experimental approaches for pursuing measurements on dilute proteins in heterogeneous mixtures.
authors.affiliation: Department of Biochemistry and Molecular Biology, McKnight Brain Institute, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Seth A
authors.initials: SA
authors.lastname: McNeill
authors.forename: Peter L
authors.initials: PL
authors.lastname: Gor'kov
authors.forename: Jochem
authors.initials: J
authors.lastname: Struppe
authors.forename: William W
authors.initials: WW
authors.lastname: Brey
authors.forename: Joanna R
authors.initials: JR
authors.lastname: Long
mesh_terms: D002247:Carbon Isotopes; D060526:Magnetic Fields; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D011506:Proteins / Q000737:chemistry*; D012107:Research Design / Q000592:standards*; D012996:Solutions
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011506:Proteins; D012996:Solutions