Comet assay of fresh and cryopreserved bull spermatozoa.
In this study, we describe DNA fragmentation of fresh and cryopreserved bull spermatozoa using the comet assay. Cryopreservation caused a significant but low (3.8%) decrease in the percentage of DNA in the comet head and an increase (5.3%) in the tail length. Our results suggest that in addition to motility and viability, low levels of DNA fragmentation after cryopreservation is a characteristic of bull spermatozoa and can be a part of remarkable cryoresistance of bull spermatozoa.
D000818:Animals; D002417:Cattle; D020552:Comet Assay; D015925:Cryopreservation / Q000662:veterinary*; D053938:DNA Fragmentation; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology*