title: Cadmium, zinc and copper biosorption mediated by Pseudomonas veronii 2E.
abstract: Adsorption properties of bacterial biomass were tested for Cd removal from liquid effluents. Experimental conditions (pH, time, cellular mass, volume, metal concentration) were studied to develop an efficient biosorption process with free or immobilised cells of Pseudomonas veronii 2E. Surface fixation was chosen to immobilise cells on inert surfaces including teflon membranes, silicone rubber and polyurethane foam. Biosorption experiments were carried out at 32 degrees C and controlled pH; maximal Cd(II) retention was observed at pH 7.5. The isotherm followed the Langmuir model (K(d)=0.17 mM and q(max)=0.48 mmol/g cell dry weight). Small changes in the surface negative charge of cells were observed by electrophoretic mobility experiments in presence of Cd(II). In addition, biosorption of 40% Cu(II) (pH 5 and 6.2) and 50% Zn(II) and 50% Cd(II) (pH 7.5) was observed from mixtures of Cu(II), Zn(II) and Cd(II) 0.5mM each.
authors.affiliation: Area Química, Instituto de Ciencias, Universidad Nacional de General Sarmiento, J.M. Gutiérrez 1150, (B1613GSX) Los Polvorines, Buenos Aires, Argentina. dvullo@ungs.edu.ar
authors.forename: Diana L
authors.initials: DL
authors.lastname: Vullo
authors.forename: Helena M
authors.initials: HM
authors.lastname: Ceretti
authors.forename: María Alejandra
authors.initials: MA
authors.lastname: Daniel
authors.forename: Silvana A M
authors.initials: SA
authors.lastname: Ramírez
authors.forename: Anita
authors.initials: A
authors.lastname: Zalts
mesh_terms: D000327:Adsorption; D002104:Cadmium / Q000378:metabolism* / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D018914:Cells, Immobilized / Q000378:metabolism; D003300:Copper / Q000378:metabolism* / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D011549:Pseudomonas / Q000187:drug effects / Q000378:metabolism*; D014873:Water Pollutants / Q000302:isolation & purification*; D015032:Zinc / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D014873:Water Pollutants; D002104:Cadmium; D003300:Copper; D015032:Zinc