title: Nitric oxide production inhibitory activity of flavonoids contained in trunk exudates of Dalbergia sissoo.
abstract: Methanolic extracts of trunk exudates of Dalbergia sissoo yielded two new open-chain neoflavonoids (1, 2), a new flavonoid (3), a new flavanone (4), and 26 known compounds. Their structures were elucidated by detailed spectroscopic analyses. The ability of the isolated compounds to prevent nitric oxide (NO) production by LPS-stimulated J774.1 cells was also studied. All of the isolated compounds except 4, formononetin, and zenognosin B exhibited significant activity in a concentration-dependent manner. Compounds 2 and 3 were among the most potent NO production inhibitors, with IC50 values of 3.19 and 6.22 microM, respectively, and compound 1 had an IC50 of 31.6 microM.
authors.affiliation: Kyoritsu University of Pharmacy, Tokyo, Japan.
authors.forename: Suraj Prakash
authors.initials: SP
authors.lastname: Shrestha
authors.forename: Yuri
authors.initials: Y
authors.lastname: Amano
authors.forename: Yuji
authors.initials: Y
authors.lastname: Narukawa
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Takeda
mesh_terms: D000818:Animals; D035782:Dalbergia / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D009390:Nepal; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase