title: Preliminary study on biomarkers for the fungal resistance in Vitis vinifera leaves.
abstract: We examined the leaf chemical composition of six seedlings obtained by self-pollination of the Bulgarian wine-making variety Storgozia as well as the cultivar Bouquet, which is the susceptible parent of Storgozia. The chemical composition was investigated in the framework of a program for identification of metabolites associated with disease resistance in grape-vine. Acetone, dichloromethane and butanol extracts, as well as volatiles obtained from fresh material were analyzed by GC/MS. Based on the correlations of the GC/MS data and estimated resistance of the leaves towards the etiological agents of powdery mildew, downy mildew and botrytis as biomarkers for the fungal resistance, we proposed 16 individual metabolites--alpha- and gamma-tocopherol, squalene, alpha-amyrine, stigmasta-3,5-diene-7-one, hexahydrofarnesyl acetone, glycolic acid, 3-hydroxybutanoic acid, 3-hydroxycaproic acid, malic acid, tartaric acid, erythronic acid, arabinoic acid, monoethyl phosphate, undecyl laurate and isopropyl myristate. The obtained correlations were confirmed by cluster analysis.
authors.affiliation: Institute of Organic Chemistry, Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. Bl. 9, Sofia 1113, Bulgaria. danibat@orgchm.bas.bg
authors.forename: Daniela Ilieva
authors.initials: DI
authors.lastname: Batovska
authors.forename: Iva Todorova
authors.initials: IT
authors.lastname: Todorova
authors.forename: Daniela Valentinova
authors.initials: DV
authors.lastname: Nedelcheva
authors.forename: Stoyan Parushev
authors.initials: SP
authors.lastname: Parushev
authors.forename: Atanas Ivanov
authors.initials: AI
authors.lastname: Atanassov
authors.forename: Tzvetanka Dimitrova
authors.initials: TD
authors.lastname: Hvarleva
authors.forename: Galina Jordanova
authors.initials: GJ
authors.lastname: Djakova
authors.forename: Vassya Stefanova
authors.initials: VS
authors.lastname: Bankova
authors.forename: Simeon Simeonov
authors.initials: SS
authors.lastname: Popov
mesh_terms: D015415:Biomarkers / Q000032:analysis; D005658:Fungi / Q000502:physiology*; D008401:Gas Chromatography-Mass Spectrometry; D007113:Immunity, Innate / Q000276:immunology*; D010802:Phylogeny; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology*; D027843:Vitis / Q000382:microbiology*
chemical_list: D015415:Biomarkers