title: The effect of aquaporin 5 overexpression on the Ras signaling pathway.
abstract: Human aquaporin 5 (AQP5) has been shown to be overexpressed in multiple cancers, such as pancreatic cancer and colon cancer. Furthermore, it has been reported that ectopic expression of AQP5 leads to many phenotypic changes characteristic of transformation. However, the biochemical mechanism leading to transformation in AQP5-overexpressing cells has not been clearly elucidated. In this report, the overexpression of AQP5 in NIH3T3 cells demonstrated a significant effect on Ras activity and, thus, cell proliferation. Furthermore, this influence was shown to be mediated by phosphorylation of the PKA consensus site of AQP5. This is the first evidence demonstrating an association between AQP5 and a signaling pathway, namely the Ras signal transduction pathway, which may be the basis of the oncogenic properties seen in AQP-overexpressing cells.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Johns Hopkins Medical Institution, Cancer Research Building II, 5M3, 1550 Orleans Street, Baltimore, MD, 21205, USA.
authors.forename: Janghee
authors.initials: J
authors.lastname: Woo
authors.forename: Juna
authors.initials: J
authors.lastname: Lee
authors.forename: Myoung Sook
authors.initials: MS
authors.lastname: Kim
authors.forename: Se Jin
authors.initials: SJ
authors.lastname: Jang
authors.forename: David
authors.initials: D
authors.lastname: Sidransky
authors.forename: Chulso
authors.initials: C
authors.lastname: Moon
mesh_terms: D000818:Animals; D051402:Aquaporin 5 / Q000378:metabolism*; D051379:Mice; D015398:Signal Transduction / Q000502:physiology*; D015854:Up-Regulation / Q000502:physiology; D018631:ras Proteins / Q000378:metabolism*
chemical_list: C496038:Aqp5 protein, mouse; D051402:Aquaporin 5; D018631:ras Proteins