title: Interferon-gamma, interleukin-10, Intercellular adhesion molecule-1, and chemokine receptor 5, but not interleukin-4, attenuate the development of periapical lesions.
abstract: This study examines the role of Th1 (interferon-gamma [IFN-gamma]) and Th2 (interleukin-4 [IL-4] and IL-10) cytokines, an intercellular adhesion molecule (ICAM-1), and a chemokine receptor (CCR5) in the pathogenesis of periapical lesions at different stages of development in knockout mice. For lesion induction, the first molar was opened and inoculated with 4 bacterial strains and left open to the oral environment. After 21 and 42 days, the IFN-gamma, IL-10, ICAM-1, and CCR5 knockout animals presented periapical lesions larger than those of wild-type animals. There was no statistically significant difference between periapical lesions induced in IL-4 knockout and wild-type animals during the periods evaluated. Our findings suggest an important role for IFN-gamma, IL-10, ICAM-1, and CCR5 in the pathogenesis of experimentally induced pulp infection and bone destruction as endogenous suppressor of periapical lesion development, whereas IL-4 appears to present a nonsignificant effect on periapical lesion modulation.
authors.affiliation: Department of Pathology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. andiaraderossi@bol.com.br
authors.forename: Andiara
authors.initials: A
authors.lastname: De Rossi
authors.forename: Lenaldo B
authors.initials: LB
authors.lastname: Rocha
authors.forename: Marcos A
authors.initials: MA
authors.lastname: Rossi
mesh_terms: D000818:Animals; D003786:Dental Pulp Cavity / Q000378:metabolism / Q000382:microbiology; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D007371:Interferon-gamma / Q000502:physiology*; D016753:Interleukin-10 / Q000502:physiology*; D015847:Interleukin-4 / Q000502:physiology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010010:Osteoclasts / Q000166:cytology; D010483:Periapical Diseases / Q000378:metabolism* / Q000382:microbiology; D019713:Receptors, CCR5 / Q000502:physiology*; D019707:Receptors, Chemokine / Q000032:analysis / Q000172:deficiency / Q000378:metabolism
chemical_list: D019713:Receptors, CCR5; D019707:Receptors, Chemokine; D018799:Intercellular Adhesion Molecule-1; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma