title: Cy3-3-acylcholine: a fluorescent analogue of acetylcholine for single molecule detection.
abstract: We synthesized a novel fluorescent analogue of acetylcholine, Cy3-3-acylcholine. The molecular weight of the products agreed with structural predictions. Discrete intensity changes of fluorescent spots due to a single ligand binding/unbinding to nAChR were visualized by TIRF microscopy. The agonist effect of the Cy3-3-acylcholine on nicotinic acetylcholine receptor (nAChR) was confirmed electrophysiologically. This newly synthesized fluorescent analogue will enable us to conduct more elaborate studies on single channel interaction processes between nAChR and ligands.
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1, Asahidai, Nomi, Ishikawa 923-1292, Japan.
authors.forename: Kenzo
authors.initials: K
authors.lastname: Fujimoto
authors.forename: Yoshinaga
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: Makoto
authors.initials: M
authors.lastname: Ihara
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Matsuda
authors.forename: Yuko
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: Takaaki
authors.initials: T
authors.lastname: Aoki
authors.forename: Toru
authors.initials: T
authors.lastname: Ide
mesh_terms: D000109:Acetylcholine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D018678:Cholinergic Agents; D005453:Fluorescence; D007473:Ion Channels / Q000378:metabolism*; D008954:Models, Biological; D015394:Molecular Structure; D009865:Oocytes; D011978:Receptors, Nicotinic / Q000378:metabolism*; D014981:Xenopus
chemical_list: D018678:Cholinergic Agents; C527386:Cy3-3-acylcholine; D007473:Ion Channels; D011978:Receptors, Nicotinic; D001285:Atropine; D000109:Acetylcholine