title: The LysR-type transcriptional regulator LeuO controls expression of several genes in Salmonella enterica serovar Typhi.
abstract: LeuO is a LysR-type transcriptional regulator that has been implicated in the bacterial stringent response and in the virulence of Salmonella. A genomic analysis with Salmonella enterica serovar Typhi revealed that LeuO is a positive regulator of OmpS1, OmpS2, AssT, and STY3070. In contrast, LeuO down-regulated the expression of OmpX, Tpx, and STY1978. Transcriptional fusions supported the positive and negative LeuO regulation. Expression of ompS1, assT, and STY3070 was induced in an hns mutant, consistent with the notion that H-NS represses these genes; transcriptional activity was lower for tpx and STY1978 in an hns background, suggesting that this global regulatory protein has a positive effect. In contrast, ompS2 and ompX expression appeared to be H-NS independent. LeuO specifically bound to the 5' intergenic regions of ompS2, assT, STY3070, ompX, and tpx, while it was not observed to bind to the promoter region of STY1978, suggesting that LeuO regulates in direct and indirect ways. In this work, a novel set of genes belonging to the LeuO regulon are described; interestingly, these genes are involved in a variety of biological processes, suggesting that LeuO is a global regulator in Salmonella.
authors.affiliation: Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Cuernavaca, Morelos 62210, México.
authors.forename: I
authors.initials: I
authors.lastname: Hernández-Lucas
authors.forename: A L
authors.initials: AL
authors.lastname: Gallego-Hernández
authors.forename: S
authors.initials: S
authors.lastname: Encarnación
authors.forename: M
authors.initials: M
authors.lastname: Fernández-Mora
authors.forename: A G
authors.initials: AG
authors.lastname: Martínez-Batallar
authors.forename: H
authors.initials: H
authors.lastname: Salgado
authors.forename: R
authors.initials: R
authors.lastname: Oropeza
authors.forename: E
authors.initials: E
authors.lastname: Calva
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D019295:Computational Biology; D018983:DNA Footprinting; D003850:Deoxyribonuclease I / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D015964:Gene Expression Regulation, Bacterial; D018272:Porins; D011401:Promoter Regions, Genetic; D018085:Regulon; D012485:Salmonella typhi / Q000235:genetics* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015534:Trans-Activators / Q000235:genetics*; D024363:Transcription Initiation Site
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C094202:OmpS1 protein, Salmonella typhi; D018272:Porins; D015534:Trans-Activators; D015500:Chloramphenicol O-Acetyltransferase; D003850:Deoxyribonuclease I