title: Expression profiling of microRNA using oligo DNA arrays.
abstract: After 12 years from its first application, microarray technology has become the reference technique to monitor gene expression of thousands of genes in the same experiment. In the past few years an increasing amount of evidence showed the importance of non-coding RNA (ncRNA) in different human diseases. The microRNAs (miRNAs) are one of the groups of ncRNA. They are small RNA fragments, 19-25 nucleotides long, with a main regulatory function on both protein coding genes and non-coding RNAs. The application of microarray platforms applied to miRNA profiling determined their deregulation in virtually all human diseases that have been studied. We previously developed a custom miRNA microarray platform, and here we describe the protocol we used to work with it including the oligo design strategy, the microarray printing protocol, the target-probe hybridization and the signal detection.
authors.affiliation: Department of Molecular Virology, Immunology and Medical Genetics and Comprehensive Cancer Center, Ohio State University, Wiseman Hall Room 385, 400 12th Avenue, Columbus, OH 43210, United States.
authors.forename: Chang-Gong
authors.initials: CG
authors.lastname: Liu
authors.forename: Riccardo
authors.initials: R
authors.lastname: Spizzo
authors.forename: George Adrian
authors.initials: GA
authors.lastname: Calin
authors.forename: Carlo Maria
authors.initials: CM
authors.lastname: Croce
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
chemical_list: D035683:MicroRNAs