title: Early development in utero of bovine nuclear transfer embryos using early G1 and G0 phase cells.
abstract: Bovine somatic cell nuclear transfer (NT) embryos can develop to normal calves, but the success rates are still quite low. Recently, enhanced development of bovine NT embryos to full term has been achieved using fibroblasts at the early G1 phase instead of cells at the quiescent (G0) phase. In the present study, we examined the morphological development in utero of NT embryos using early G1 phase cells (eG1-NT embryos) and G0 phase cells (G0-NT embryos). We produced eG1- and G0-NT blastocysts, and then they were transferred to recipient heifers for transient development in utero up to day 14 of gestation. In vitro-fertilized (IVF), parthenogenetic and artificially inseminated (AI) embryos were used as controls. The rate of formation of embryonic disks of the recovered embryos was the same among the groups of eG1-NT, IVF, and AI embryos (p>0.05). The formation rate in eG1-NT embryos was significantly higher than that in G0-NT embryos (p<0.05). The lengths of eG1-NT embryos were the same as those of IVF, parthenogenetic, and AI embryos (p>0.05), but significantly shorter than those of G0-NT embryos (p<0.01). We conclude that the morphological development of day 14 embryos derived from eG1-NT embryos was mostly similar to that of AI embryos, but that the morphological development of G0-NT embryos was abnormally large and different from that of AI and eG1-NT embryos.
authors.affiliation: Zen-noh Embryo Transfer Center, Kamishihoro, Katogun, Hokkaido, Japan. ideta-atsushi@zennoh.or.jp
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ideta
authors.forename: Manami
authors.initials: M
authors.lastname: Urakawa
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Aoyagi
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Saeki
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D002453:Cell Cycle; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro; D016193:G1 Phase; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal; D016192:Resting Phase, Cell Cycle