title: Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis: implications for catalytic mechanism.
abstract: The methionine salvage pathway (MSP) plays a crucial role in recycling a sulphahydryl derivative of the nucleoside. Recently, the genes and reactions in MSP from Bacillus subtilis have been identified, where 5-methylthioribose 1-phosphate isomerase (M1Pi) catalyzes a conversion of 5-methylthioribose 1-phosphate (MTR-1-P) to 5-methylthioribulose 1-phosphate (MTRu-1-P). Herein, we report the crystal structures of B. subtilis M1Pi (Bs-M1Pi) in complex with its product MTRu-1-P, and a sulfate at 2.4 and 2.7 A resolution, respectively. The electron density clearly shows the presence of each compound in the active site. The structural comparison with other homologous proteins explains how the substrate uptake of Bs-M1Pi may be induced by an open/closed transition of the active site. The highly conserved residues at the active site, namely, Cys160 and Asp240 are most likely to be involved in catalysis. The structural analysis sheds light on its catalytic mechanism of M1Pi.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Osaka, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Tamura
authors.forename: Yohtaro
authors.initials: Y
authors.lastname: Saito
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ashida
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kai
authors.forename: Akiho
authors.initials: A
authors.lastname: Yokota
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000737:chemistry* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry; D001665:Binding Sites; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation
chemical_list: D001426:Bacterial Proteins; D019747:Aldose-Ketose Isomerases; C526965:5-methylthioribose 1-phosphate isomerase, Bacillus subtilis