title: Effect of low-level laser therapy on skin fibroblasts of streptozotocin-diabetic rats.
abstract: OBJECTIVE
  This study explored the effects of low-level laser therapy (LLLT) on cellular changes in cell culture and organ culture of skin from streptozotocin-diabetic (STZ-D) rats.
  
  
  BACKGROUND
  Growth of skin and its fibroblasts are impaired in diabetes. Therefore the healing of skin wounds is impaired in diabetic patients. The positive effects of LLLT on complications of diabetes in patients and animal models have been shown.
  
  
  METHODS
  Diabetes was induced in rats by streptozotocin 30 days after its injection. Two sets of skin samples were extracted from skin under sterile conditions. Fibroblasts that were extruded from the samples were proliferated in vitro, and another set of samples were cultured as organ culture. A 24-well culture medium containing Dulbecco's modified minimum essential medium was supplemented by 12% fetal bovine serum. There were five laser-treated and five sham-exposed groups. A helium-neon laser was used, and 0.9-4 J/cm(2) energy densities were applied four times to each organ culture and cell culture. The organ cultures were analyzed by light microscopy and transmission electron microscopy examinations. Cell proliferation was evaluated by dimethylthiazol-diphenyltetrazolium bromide (MTT) assay.
  
  
  RESULTS
  Statistical analysis revealed that 4-J/cm(2) irradiation significantly increases the fibroblast numbers compared to the sham-exposed cultures (p = 0.046).
  
  
  CONCLUSIONS
  It is concluded that LLLT resulted in a significant increase of fibroblast proliferation of STZ-D rats in vitro.
authors.affiliation: Avicenna Hospital, Mazandran Medical University, Sari, Iran.
authors.forename: Mansoreh
authors.initials: M
authors.lastname: Mirzaei
authors.forename: Mohammad
authors.initials: M
authors.lastname: Bayat
authors.forename: Nariman
authors.initials: N
authors.lastname: Mosafa
authors.forename: Zhale
authors.initials: Z
authors.lastname: Mohsenifar
authors.forename: Abbas
authors.initials: A
authors.lastname: Piryaei
authors.forename: Babak
authors.initials: B
authors.lastname: Farokhi
authors.forename: Fatemehsadat
authors.initials: F
authors.lastname: Rezaei
authors.forename: Yousef
authors.initials: Y
authors.lastname: Sadeghi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Rakhshan
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000528:radiation effects; D048909:Diabetes Complications / Q000532:radiotherapy; D003921:Diabetes Mellitus, Experimental / Q000473:pathology / Q000532:radiotherapy*; D005347:Fibroblasts / Q000528:radiation effects*; D028022:Low-Level Light Therapy; D008297:Male; D009924:Organ Culture Techniques; D051381:Rats; D014147:Tramadol / Q000627:therapeutic use; D014945:Wound Healing / Q000528:radiation effects
chemical_list: D014147:Tramadol