title: Gene silencing of ribosomal protein P0 is lethal to the tick Haemaphysalis longicornis.
abstract: Ribosomal protein P0 has been demonstrated to be a multifunctional protein in the large subunit of eukaryotic ribosome. In this study, a gene encoding ribosomal protein P0 termed HlP0 was isolated from a full-length salivary gland cDNA library previously constructed from the tick Haemaphysalis longicornis. The full-length cDNA of the HlP0 gene is 1141 bp, with an open reading frame (ORF) of 963 bp. The ORF of the HlP0 gene encodes a putative protein of 320 amino acid residues, with a predicted molecular mass of 35 kDa. Reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated that the HlP0 gene transcript was expressed in all developmental stages and all tissues dissected from 4-day-fed adult ticks. Antibodies raised against recombinant HlP0 recognized a native protein with an expected molecular size of 35 kDa in all tested tissues. RNA interference of HlP0 gene was carried out by injecting ticks with PBS, green fluorescent protein (GFP) dsRNA, and HlP0 dsRNA. The results showed that ticks treated with HlP0 dsRNA obtained a strikingly lower body weight (2.63+/-1.21 mg vs. 226.75+/-74.80 mg in the PBS-injected group and 231.15+/-51.32 mg in the GFP dsRNA-injected group, Student's t-test, P<0.01), a lower engorgement rate (4% vs. 100% and 94.11%, respectively), and higher mortality (96% vs. 2.5% and 10.4%, respectively) after blood-sucking than the control groups. This suggests that ribosomal protein P0 is required for the blood ingestion and subsequent viability of H. longicornis. This is the first report of ribosomal protein P0 from ticks.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Gong
authors.forename: Min
authors.initials: M
authors.lastname: Liao
authors.forename: Jinlin
authors.initials: J
authors.lastname: Zhou
authors.forename: Tekeshi
authors.initials: T
authors.lastname: Hatta
authors.forename: Penglong
authors.initials: P
authors.lastname: Huang
authors.forename: Guohong
authors.initials: G
authors.lastname: Zhang
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Kanuka
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
authors.forename: Kozo
authors.initials: K
authors.lastname: Fujisaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000378:metabolism; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry; D020869:Gene Expression Profiling / Q000662:veterinary; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000032:analysis; D026863:Ixodidae / Q000235:genetics*; D008969:Molecular Sequence Data; D034622:RNA Interference / Q000502:physiology*; D012330:RNA, Double-Stranded / Q000008:administration & dosage / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D012269:Ribosomal Proteins / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D012469:Salivary Glands / Q000187:drug effects; D016415:Sequence Alignment / Q000662:veterinary
chemical_list: D000906:Antibodies; D018076:DNA, Complementary; D012330:RNA, Double-Stranded; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C054675:ribosomal protein P0; D049452:Green Fluorescent Proteins