title: Molecular cloning of leucocyte cell-derived chemotaxin-2 gene in croceine croaker (Pseudosciaena crocea).
abstract: Leucocyte cell-derived chemotaxin-2 (LECT2) was originally demonstrated to have a chemotactic activity against human neutrophils in vitro. Current evidence suggests that LECT2 may be a multifunctional protein involved in cell growth, differentiation and autoimmune. A full-length cDNA clone of the LECT2 gene, 595bp in size, was isolated from the fish croceine croaker (Pseudosciaena crocea). It's 3'-UTR was much shorter (112nts) than that of trout LECT2 gene (210nts). Its deduced amino acid sequence of 151 residues had 39.7-75.5% identity to that of other animals. Phylogenetic analysis shows that croceine croaker LECT2 (pLECT2) is clustered tightly with other fish LECT2. The relationships of the different LECT2 coincided well with the evolutionary relationships of their organisms. In healthy fish, the expression levels of pLECT2 gene from different tissues were similar, while that in Vibrio alginolyticus-infected fish were significantly increased in liver and spleen comparing to those in healthy fish, and were a little higher in the other tissues.
authors.affiliation: Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo City, Zhejiang Province, PR China.
authors.forename: Ming Y
authors.initials: MY
authors.lastname: Li
authors.forename: Jiong
authors.initials: J
authors.lastname: Chen
authors.forename: Yu H
authors.initials: YH
authors.lastname: Shi
mesh_terms: D000199:Actins / Q000032:analysis / Q000096:biosynthesis; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D005786:Gene Expression Regulation / Q000276:immunology*; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics*; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014735:Vibrio Infections / Q000276:immunology*; D044149:Vibrio alginolyticus / Q000276:immunology
chemical_list: D000199:Actins; D018076:DNA, Complementary; D036341:Intercellular Signaling Peptides and Proteins