title: Cholinergic regulation of neuropeptide Y synthesis and release in human neuroblastoma cells.
abstract: The biosynthesis and release of neuropeptide Y (NPY) is regulated by several factors. Here, the effect of the muscarinic agonist carbachol on NPY biosynthesis and release was analyzed utilizing the SH-SY5Y human neuroblastoma cell line. We observed that: (a) carbachol moderately increased the post-translational cleavage of proNPY to NPY; (b) carbachol treatment stimulated NPY accumulation into the medium in a time- and dose-related manner; (c) protein kinase C activation is involved in carbachol-mediated NPY synthesis/release (>6h). In conclusion, the present observations support the hypothesis that muscarinic receptor activation regulates the biosynthesis and secretion of NPY.
authors.affiliation: Istituto di Endocrinologia, Center of Excellence on Neurodegenerative Diseases and Center for Endocrinological Oncology, University of Milan, Milano, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Dozio
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Ruscica
authors.forename: Daniel
authors.initials: D
authors.lastname: Feltrin
authors.forename: Marcella
authors.initials: M
authors.lastname: Motta
authors.forename: Paolo
authors.initials: P
authors.lastname: Magni
mesh_terms: D002217:Carbachol / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology; D009478:Neuropeptide Y / Q000096:biosynthesis*; D011493:Protein Kinase C / Q000378:metabolism; D013997:Time Factors
chemical_list: D009478:Neuropeptide Y; D002217:Carbachol; D011493:Protein Kinase C