title: Investigation of beta-lactamase-producing multidrug-resistant Pseudomonas aeruginosa isolated from non-tertiary care hospitals in Korea.
abstract: A total of 2,280 nonduplicate clinical isolates of Pseudomonas aeruginosa, obtained nationwide from Korean non-tertiary care hospitals from 2002 to 2005, were identified and their susceptibilities to aminoglycosides, antipseudomonal penicillins, carbapenems, cephalosporins, monobactams, and quinolones were studied, together with their production of beta- lactamases. Using disk diffusion and minimum inhibitory concentration tests, it was found that 2.9% of isolates were multidrug-resistant (MDR) P. aeruginosa. An EDTA-disk synergy test, PCR amplification with specifically designed primers, and direct sequencing of the PCR products showed that the blaOXA-10, blaVIM-2, blaOXA-2, blaOXA-17, blaPER-1, blaSHV-12, and blaIMP-1 genes were carried by 34.3%, 26.9%, 3.0%, 3.0%, 1.5%, 1.5%, and 1.5% of 67 MDR P. aeruginosa isolates, respectively. The prevalence of MDR P. aeruginosa was three-fold higher, compared with that from the United States. More than two types of beta-lactamase genes were carried by 10.4% of isolates. The most prevalent beta-lactamase genes were blaVIM-2 and blaOXA-10. This study is the first description of MDR P. aeruginosa from non-tertiary care hospitals in Korea and the coexistence of the blaOXA-10 gene with blaVIM-2, blaIMP-1, or blaPER-1 in these clinical isolates.
authors.affiliation: Division of Antimicrobial Resistance, Center for Infectious Disease Research, National Institute of Health, Seoul, Korea.
authors.forename: Sohn Eui
authors.initials: SE
authors.lastname: Suk
authors.forename: Jeong Sik
authors.initials: JS
authors.lastname: Yoo
authors.forename: Jeom Kyu
authors.initials: JK
authors.lastname: Lee
authors.forename: Kyeong Min
authors.initials: KM
authors.lastname: Lee
authors.forename: Gyung Tae
authors.initials: GT
authors.lastname: Chung
authors.forename: Eun Shim
authors.initials: ES
authors.lastname: Shin
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Han
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Joon
authors.initials: J
authors.lastname: Kim
authors.forename: Yeong Seon
authors.initials: YS
authors.lastname: Lee
mesh_terms: D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006761:Hospitals; D006801:Humans; D007723:Korea; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics*
chemical_list: D001618:beta-Lactamases