title: Selection of pH-related parameters in ion-exchange chromatography using pH-gradient operations.
abstract: This work demonstrates that the type of ion-exchanger (anion or cation), the mode of operation (bind-and-elute or flow-through), and the operational pH of ion-exchange chromatography (IEX) can be selected in a fast and rational way by analytical pH-gradient IEX operations, thereby eliminating the need for pH scouting or high-throughput screening. The developed approach was applied for the selection of an IEX process for the capture of a monoclonal antibody (MAb) from hybridoma cell culture supernatant (CCS). It was found within a day that MAb can optimally be captured by bind-and-elute mode cation-exchange chromatography (CEX) at pH 4.5 or anion-exchange chromatography (AEX) at pH 7.2 without lowering the salt concentration in the CCS. The performance of both CEX and AEX was predicted to be equal for this particular MAb capture.
authors.affiliation: Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.
authors.forename: Tangir
authors.initials: T
authors.lastname: Ahamed
authors.forename: Sreekanth
authors.initials: S
authors.lastname: Chilamkurthi
authors.forename: Beckley K
authors.initials: BK
authors.lastname: Nfor
authors.forename: Peter D E M
authors.initials: PD
authors.lastname: Verhaert
authors.forename: Gijs W K
authors.initials: GW
authors.lastname: van Dedem
authors.forename: Luuk A M
authors.initials: LA
authors.lastname: van der Wielen
authors.forename: Michel H M
authors.initials: MH
authors.lastname: Eppink
authors.forename: Emile J A X
authors.initials: EJ
authors.lastname: van de Sandt
authors.forename: Marcel
authors.initials: M
authors.lastname: Ottens
mesh_terms: D000837:Anion Exchange Resins; D002411:Cation Exchange Resins; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration
chemical_list: D000837:Anion Exchange Resins; D002411:Cation Exchange Resins