title: Mutation at different sites in the Nostoc punctiforme cyaC gene, encoding the multiple-domain enzyme adenylate cyclase, results in different levels of infection of the host plant Blasia pusilla.
abstract: The filamentous cyanobacterium Nostoc punctiforme forms symbioses with plants. Disruption of the catalytic domain of the N. punctiforme adenylate cyclase (CyaC) significantly increased symbiotic competence, whereas reduced infectivity was observed in a mutant with a disruption close to the N terminus of CyaC. The total cellular cyclic AMP levels were significantly reduced in both mutants.
authors.affiliation: Institute of Integrative and Comparative Biology, Garstang Building, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom.
authors.forename: Karen E
authors.initials: KE
authors.lastname: Chapman
authors.forename: Paula S
authors.initials: PS
authors.lastname: Duggan
authors.forename: Natalie A
authors.initials: NA
authors.lastname: Billington
authors.forename: David G
authors.initials: DG
authors.lastname: Adams
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D035926:Hepatophyta / Q000382:microbiology*; D008957:Models, Genetic; D009154:Mutation; D046937:Nostoc / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D010935:Plant Diseases / Q000382:microbiology; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D000242:Cyclic AMP; D000262:Adenylyl Cyclases