title: [Electrophoretic patterns of salivary hemeproteins (nitrophorines) of Rhodnius colombiensis and R. prolixus (Hemiptera, Reduviidae, Triatominae)].
abstract: BACKGROUND
  Salivary hemeprotein electrophoresis in starch gels is a recent technique used for differentiation of Rhodnius species with great phenotypic similarity. Furthermore, populations of the same species can be differentiated from geographically separated locales. Of the 15 described Rhodnius species in Latin America, at least eight have been reported in Colombia.
  
  
  OBJECTIVE
  To use the salivary hemeproteins electrophoresis for R. prolixus and R. colombiensis identification. These two species are phenotypically similar and have overlapping domestic and sylvatic cycles where they occur in the upper basin of the Magdalena river, Central Colombia.
  
  
  METHODS
  The content of salivary glands of each insect was subjected to starch gel electrophoresis using glycine buffer, and the bands were revealed with 3,3',5,5'-tetramethylbenzidine. Band patterns were photographically recorded.
  
  
  RESULTS
  Electrophoretic patterns of salivary hemeproteins of R. prolixus and R. colombiensis were able to unequivocally differentiate the two species.
  
  
  CONCLUSIONS
  The usefulness of the starch gel technique for distinguishing between R. prolixus and R. colombiensis was demonstrated as an additional tool to the morphometric and molecular methods already in use for differentiation of these two species.
authors.affiliation: Laboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Universidad del Tolima, Ibagué, Colombia.
authors.forename: Andrea
authors.initials: A
authors.lastname: Arévalo
authors.forename: Julio César
authors.initials: JC
authors.lastname: Carranza
authors.forename: Felipe
authors.initials: F
authors.lastname: Guhl
authors.forename: Gustavo Adolfo
authors.initials: GA
authors.lastname: Vallejo
mesh_terms: D000818:Animals; D004592:Electrophoresis, Starch Gel; D006420:Hemeproteins / Q000032:analysis* / Q000235:genetics; D019476:Insect Proteins / Q000032:analysis* / Q000235:genetics; D011110:Polymorphism, Genetic; D012239:Rhodnius / Q000033:anatomy & histology / Q000737:chemistry* / Q000145:classification; D012469:Salivary Glands / Q000737:chemistry; D012471:Salivary Proteins and Peptides / Q000032:analysis* / Q000235:genetics
chemical_list: D006420:Hemeproteins; D019476:Insect Proteins; D012471:Salivary Proteins and Peptides
vernacular_title: Patrones electroforéticos de hemoproteínas salivares (nitroforinas) de Rhodnius colombiensis y Rhodnius prolixus (Hemiptera, Reduviidae, Triatominae).