Paramagnetic relaxation-based 19f MRI probe to detect protease activity.
A novel design principle for 19F MRI probes detecting protease activity was developed. This principle is based on 19F MRI signal quenching by the intramolecular paramagnetic effect from Gd3+. The intramolecular Gd3+ dramatically attenuated the 19F probe signal, and the paramagnetic effect was cancelled by the probe hydrolyzation by caspase-3. Using this probe, it was shown that the probe could detect caspase-3 activity spatially from a phantom image using 19F MRI.
D000818:Animals; D053148:Caspase 3 / Q000032:analysis; D005461:Fluorine / Q000737:chemistry*; D006801:Humans; D007554:Isotopes / Q000737:chemistry; D015335:Molecular Probes / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000032:analysis*
D007554:Isotopes; D015335:Molecular Probes; D009842:Oligopeptides; D009942:Organometallic Compounds; C526603:gadolinum-DOTA-aspartyl-glutamyl-valyl-aspartic acid 4-trifluoromethoxybenzylamide; D005461:Fluorine; D010447:Peptide Hydrolases; D053148:Caspase 3