title: Paramagnetic relaxation-based 19f MRI probe to detect protease activity.
abstract: A novel design principle for 19F MRI probes detecting protease activity was developed. This principle is based on 19F MRI signal quenching by the intramolecular paramagnetic effect from Gd3+. The intramolecular Gd3+ dramatically attenuated the 19F probe signal, and the paramagnetic effect was cancelled by the probe hydrolyzation by caspase-3. Using this probe, it was shown that the probe could detect caspase-3 activity spatially from a phantom image using 19F MRI.
authors.affiliation: Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Mizukami
authors.forename: Rika
authors.initials: R
authors.lastname: Takikawa
authors.forename: Fuminori
authors.initials: F
authors.lastname: Sugihara
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Hori
authors.forename: Hidehito
authors.initials: H
authors.lastname: Tochio
authors.forename: Markus
authors.initials: M
authors.lastname: Wälchli
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shirakawa
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kikuchi
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000032:analysis; D005461:Fluorine / Q000737:chemistry*; D006801:Humans; D007554:Isotopes / Q000737:chemistry; D015335:Molecular Probes / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000032:analysis*
chemical_list: D007554:Isotopes; D015335:Molecular Probes; D009842:Oligopeptides; D009942:Organometallic Compounds; C526603:gadolinum-DOTA-aspartyl-glutamyl-valyl-aspartic acid 4-trifluoromethoxybenzylamide; D005461:Fluorine; D010447:Peptide Hydrolases; D053148:Caspase 3