title: Acute modulations in permeability barrier function regulate epidermal cornification: role of caspase-14 and the protease-activated receptor type 2.
abstract: Stratum corneum comprises corneocytes, derived from outer stratum granulosum during terminal differentiation, embedded in a lipid-enriched extracellular matrix, secreted from epidermal lamellar bodies. Permeability barrier insults stimulate rapid secretion of preformed lamellar bodies from the outer stratum granulosum, regulated through modulations in ionic gradients and serine protease (SP)/protease-activated receptor type 2 (PAR2) signaling. Because corneocytes are also required for barrier function, we hypothesized that corneocyte formation could also be regulated by barrier function. Barrier abrogation by two unrelated methods initiated a wave of cornification, assessed as TdT-mediated dUTP nick end-labeling-positive cells in stratum granulosum and newly cornified cells by electron microscopy. Because cornification was blocked by occlusion, corneocytes formed specifically in response to barrier, rather than injury or cell replacement, requirements. SP inhibitors and hyperacidification (which decreases SP activity) blocked cornification after barrier disruption. Similarly, cornification was delayed in PAR2(-/-) mice. Although classical markers of apoptosis [poly(ADP-ribose)polymerase and caspase (Casp)-3] remained unchanged, barrier disruption activated Casp-14. Moreover, the pan-Casp inhibitor Z-VAD-FMK delayed cornification, and corneocytes were structurally aberrant in Casp14(-/-) mice. Thus, permeability barrier requirements coordinately drive both the generation of the stratum corneum lipid-enriched extracellular matrix and the transformation of granular cells into corneocytes, in an SP- and Casp-14-dependent manner, signaled by PAR2.
authors.affiliation: Dermatology and Medical (Metabolism) Services, Veterans Administration Medical Center, 4510 Clement St., San Francisco, CA 94121, USA.
authors.forename: Marianne
authors.initials: M
authors.lastname: Demerjian
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Hachem
authors.forename: Erwin
authors.initials: E
authors.lastname: Tschachler
authors.forename: Geertrui
authors.initials: G
authors.lastname: Denecker
authors.forename: Wim
authors.initials: W
authors.lastname: Declercq
authors.forename: Peter
authors.initials: P
authors.lastname: Vandenabeele
authors.forename: Theodora
authors.initials: T
authors.lastname: Mauro
authors.forename: Melanie
authors.initials: M
authors.lastname: Hupe
authors.forename: Debra
authors.initials: D
authors.lastname: Crumrine
authors.forename: Truus
authors.initials: T
authors.lastname: Roelandt
authors.forename: Evi
authors.initials: E
authors.lastname: Houben
authors.forename: Peter M
authors.initials: PM
authors.lastname: Elias
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Feingold
mesh_terms: D000818:Animals; D017209:Apoptosis; D053456:Caspase 14 / Q000096:biosynthesis* / Q000378:metabolism*; D002454:Cell Differentiation; D004817:Epidermis / Q000378:metabolism*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006863:Hydrogen-Ion Concentration; D020287:In Situ Nick-End Labeling; D008297:Male; D051379:Mice; D008954:Models, Biological; D010539:Permeability; D044464:Receptor, PAR-2 / Q000378:metabolism*; D012879:Skin Physiological Phenomena
chemical_list: D044464:Receptor, PAR-2; D053456:Caspase 14