title: Comet assay of fresh and cryopreserved bull spermatozoa.
abstract: In this study, we describe DNA fragmentation of fresh and cryopreserved bull spermatozoa using the comet assay. Cryopreservation caused a significant but low (3.8%) decrease in the percentage of DNA in the comet head and an increase (5.3%) in the tail length. Our results suggest that in addition to motility and viability, low levels of DNA fragmentation after cryopreservation is a characteristic of bull spermatozoa and can be a part of remarkable cryoresistance of bull spermatozoa.
authors.affiliation: Semen Biology Group, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland.
authors.forename: Mariola
authors.initials: M
authors.lastname: Słowińska
authors.forename: Halina
authors.initials: H
authors.lastname: Karol
authors.forename: Andrzej
authors.initials: A
authors.lastname: Ciereszko
mesh_terms: D000818:Animals; D002417:Cattle; D020552:Comet Assay; D015925:Cryopreservation / Q000662:veterinary*; D053938:DNA Fragmentation; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology*