Preliminary study on biomarkers for the fungal resistance in Vitis vinifera leaves.
We examined the leaf chemical composition of six seedlings obtained by self-pollination of the Bulgarian wine-making variety Storgozia as well as the cultivar Bouquet, which is the susceptible parent of Storgozia. The chemical composition was investigated in the framework of a program for identification of metabolites associated with disease resistance in grape-vine. Acetone, dichloromethane and butanol extracts, as well as volatiles obtained from fresh material were analyzed by GC/MS. Based on the correlations of the GC/MS data and estimated resistance of the leaves towards the etiological agents of powdery mildew, downy mildew and botrytis as biomarkers for the fungal resistance, we proposed 16 individual metabolites--alpha- and gamma-tocopherol, squalene, alpha-amyrine, stigmasta-3,5-diene-7-one, hexahydrofarnesyl acetone, glycolic acid, 3-hydroxybutanoic acid, 3-hydroxycaproic acid, malic acid, tartaric acid, erythronic acid, arabinoic acid, monoethyl phosphate, undecyl laurate and isopropyl myristate. The obtained correlations were confirmed by cluster analysis.
D015415:Biomarkers / Q000032:analysis; D005658:Fungi / Q000502:physiology*; D008401:Gas Chromatography-Mass Spectrometry; D007113:Immunity, Innate / Q000276:immunology*; D010802:Phylogeny; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology*; D027843:Vitis / Q000382:microbiology*
D015415:Biomarkers