title: Anillin is a scaffold protein that links RhoA, actin, and myosin during cytokinesis.
abstract: Cell division after mitosis is mediated by ingression of an actomyosin-based contractile ring. The active, GTP-bound form of the small GTPase RhoA is a key regulator of contractile-ring formation. RhoA concentrates at the equatorial cell cortex at the site of the nascent cleavage furrow. During cytokinesis, RhoA is activated by its RhoGEF, ECT2. Once activated, RhoA promotes nucleation, elongation, and sliding of actin filaments through the coordinated activation of both formin proteins and myosin II motors (reviewed in [1, 2]). Anillin is a 124 kDa protein that is highly concentrated in the cleavage furrow in numerous animal cells in a pattern that resembles that of RhoA [3-7]. Although anillin contains conserved N-terminal actin and myosin binding domains and a PH domain at the C terminus, its mechanism of action during cytokinesis remains unclear. Here, we show that human anillin contains a conserved C-terminal domain that is essential for its function and localization. This domain shares homology with the RhoA binding protein Rhotekin and directly interacts with RhoA. Further, anillin is required to maintain active myosin in the equatorial plane during cytokinesis, suggesting it functions as a scaffold protein to link RhoA with the ring components actin and myosin. Although furrows can form and initiate ingression in the absence of anillin, furrows cannot form in anillin-depleted cells in which the central spindle is also disrupted, revealing that anillin can also act at an early stage of cytokinesis.
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA.
authors.forename: Alisa J
authors.initials: AJ
authors.lastname: Piekny
authors.forename: Michael
authors.initials: M
authors.lastname: Glotzer
mesh_terms: D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D051017:Apoptosis Regulatory Proteins; D017124:Conserved Sequence; D003285:Contractile Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D048749:Cytokinesis / Q000502:physiology*; D019204:GTP-Binding Proteins; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry; D008969:Molecular Sequence Data; D009218:Myosins / Q000032:analysis / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D008941:Spindle Apparatus / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
chemical_list: D000199:Actins; D051017:Apoptosis Regulatory Proteins; D003285:Contractile Proteins; D047908:Intracellular Signaling Peptides and Proteins; C100494:RTKN protein, human; C095886:anillin; D019204:GTP-Binding Proteins; D009218:Myosins; D020742:rhoA GTP-Binding Protein