title: Tabebuia avellanedae extracts inhibit IL-2-independent T-lymphocyte activation and proliferation.
abstract: In order to identify new, immune modulating compounds, aqueous extracts of plants pre-selected on ethno-pharmacological knowledge were screened for inhibitory effects in an anti-CD3 driven lymphocyte proliferation assay (MTT-assay). We found for the extract of the inner bark of Tabebuia avellanedae (Tabebuia) dose dependent and reproducible inhibitory effects on lymphocyte proliferation. We further analyzed Tabebuia in flow cytometry based whole blood T-cell function assays. We found that Tabebuia inhibited dose dependent ConA stimulated T-cell proliferation. Decreased T-lymphocyte proliferation was associated with dose dependent reduction of CD25 and CD71 expression on T-lymphocytes. In contrast Tabebuia exerted no effects on cytokine expression (Il-2 and TNF-alpha) by PMA/Ionomycin stimulated T-lymphocytes. Concentrations of Tabebuia used were not toxic for lymphocytes as verified by trypan blue exclusion assay. Further experiments showed that the immune inhibitory effects by Tabebuia were not mediated by its pharmacological lead compound beta-lapachone and only observed in aqueous but not in ethanol plant extracts.
authors.affiliation: INSERM U858, Institute Louis Bugnard, CHU Rangueil, Toulouse, France. boehlert@toulouse.inserm.fr
authors.forename: Torsten
authors.initials: T
authors.lastname: Böhler
authors.forename: Jochen
authors.initials: J
authors.lastname: Nolting
authors.forename: Purevtseren
authors.initials: P
authors.lastname: Gurragchaa
authors.forename: Adrian
authors.initials: A
authors.lastname: Lupescu
authors.forename: Hans-H
authors.initials: HH
authors.lastname: Neumayer
authors.forename: Klemens
authors.initials: K
authors.lastname: Budde
authors.forename: Nassim
authors.initials: N
authors.lastname: Kamar
authors.forename: Jochen
authors.initials: J
authors.lastname: Klupp
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006131:Growth Inhibitors / Q000494:pharmacology; D006801:Humans; D007108:Immune Tolerance / Q000187:drug effects* / Q000276:immunology; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007376:Interleukin-2 / Q000037:antagonists & inhibitors / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D010936:Plant Extracts / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D029663:Tabebuia / Q000276:immunology*
chemical_list: D006131:Growth Inhibitors; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D010936:Plant Extracts