title: 920 MHz ultra-high field NMR approaches to structural glycobiology.
abstract: Although NMR spectroscopy has great potential to provide us with detailed structural information on oligosaccharides and glycoconjugates, the carbohydrate NMR analyses have been hampered by the severe spectral overlapping and the insufficiency of the conformational restraints. Recently, ultra-high field NMR spectrometers have become available for applications to structural analyses of biological macromolecules. Here we demonstrate that ultra-high fields offer not only increases in sensitivity and chemical shift dispersion but also potential benefits for providing unique information on chemical exchange and relaxation, by displaying NMR spectral data of oligosaccharide, glycoprotein, and glycolipid systems recorded at a 21.6 T magnetic field (corresponding to 920 MHz (1)H observation frequency). The ultra-high field NMR spectroscopy combined with sugar library and stable-isotope labeling approaches will open new horizons in structural glycobiology.
authors.affiliation: Institute for Molecular Science, National Institutes of Natural Sciences, 5-1 Higashiyama Myodaiji, Okazaki 444-8787, Japan. kkato@phar-nagoya-cu.ac.jp
authors.forename: Koichi
authors.initials: K
authors.lastname: Kato
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sasakawa
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kamiya
authors.forename: Maho
authors.initials: M
authors.lastname: Utsumi
authors.forename: Michiko
authors.initials: M
authors.lastname: Nakano
authors.forename: Noriko
authors.initials: N
authors.lastname: Takahashi
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Yamaguchi
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry; D000818:Animals; D054794:Glycomics / Q000379:methods*; D006023:Glycoproteins / Q000737:chemistry; D006801:Humans; D007553:Isotope Labeling; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D009844:Oligosaccharides / Q000737:chemistry
chemical_list: D016229:Amyloid beta-Peptides; D006023:Glycoproteins; D009844:Oligosaccharides