title: Preparation of chitosan particles suitable for enzyme immobilization.
abstract: Macro-, micro- and nanosized chitosan particles suitable as immobilization carriers were prepared by precipitation, emulsion cross-linking and ionic gelation methods, respectively. Effects of particle preparation parameters on particle size were investigated. Activities of beta-galactosidase covalently attached to differently sized particles have been evaluated and compared. The highest activity was shown by the biocatalyst immobilized on nanoparticles obtained by means of the ionotropic gelation method with sodium sulphate as gelation agent. beta-Galactosidase fixed on macro- and microspheres exhibited excellent storage stability in aqueous solution, with no more than 5% loss of activity after 3 weeks storage at 4 degrees C and pH 7.0.
authors.affiliation: University of Pannonia, Faculty of Information Technology, Research Institute of Chemical and Process Engineering, H-8200 Veszprém, Egyetem Street 10, Hungary.
authors.forename: Emese
authors.initials: E
authors.lastname: Biró
authors.forename: Agnes Sz
authors.initials: AS
authors.lastname: Németh
authors.forename: Csaba
authors.initials: C
authors.lastname: Sisak
authors.forename: Tivadar
authors.initials: T
authors.lastname: Feczkó
authors.forename: János
authors.initials: J
authors.lastname: Gyenis
mesh_terms: D002384:Catalysis; D048271:Chitosan / Q000737:chemistry*; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D013501:Surface-Active Agents; D001616:beta-Galactosidase / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D013501:Surface-Active Agents; D048271:Chitosan; D001616:beta-Galactosidase