title: [Characterization of stem cells from the murine adult mammary gland].
abstract: The postnatal mammary morphogenesis comprises two steps, first, formation of a system of branching ducts at puberty and second, alveologenesis during pregnancy. The mammary epithelium is organized as a bilayer, composed of two cellular types, basal myoepithelial and luminal epithelial. The remarkable regenerative properties revealed in serial transplantation experiments suggest that the adult mammary epithelium harbors stem cells. Various strategies including analysis of DNA label-retaining cells, transgenic approach, and in vivo transplantation assay, have been used to isolate and characterize murine mammary stem and progenitor cells. Their molecular characteristics remain to be defined precisely but notable progress have been already made in the enrichment and identification of these cells. Current studies favor the hypothesis of a basal-type mammary stem cells expressing high levels of alpha 6, beta1 and beta 3 integrin chains, the major receptors of extracellular matrix proteins. Luminal-type progenitors may participate in the establishment of the bilayered alveolar epithelium during pregnancy.
authors.affiliation: Equipe Mécanismes moléculaires du développement de la glande mammaire, Institut Curie, CNRS UMR144, 26, rue d'Ulm, 75248 Paris Cedex 05, France. Marie-Ange.Deugnier@curie.fr
authors.forename: Marie-Ange
authors.initials: MA
authors.lastname: Deugnier
authors.forename: Valérie
authors.initials: V
authors.lastname: Petit
authors.forename: Ilaria
authors.initials: I
authors.lastname: Taddéi-De La Hosseraye
authors.forename: Marisa
authors.initials: M
authors.lastname: Faraldo
authors.forename: Marina A
authors.initials: MA
authors.lastname: Glukhova
mesh_terms: D000375:Aging; D000818:Animals; D005260:Female; D016023:Integrins / Q000235:genetics / Q000502:physiology; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000254:growth & development; D051379:Mice; D009024:Morphogenesis; D012741:Sexual Maturation; D013234:Stem Cells / Q000166:cytology*
chemical_list: D016023:Integrins
vernacular_title: Vers la caractérisation des cellules souches de la glande mammaire murine adulte.