title: Profiling of radicular cyst and odontogenic keratocyst cytokine production suggests common growth mechanisms.
abstract: The aim of this study was to compare the cytokine expression profiles of cyst fluids (CFs) and tissue culture supernatants (SUPs) from 7 radicular cysts (RCs) and 7 odontogenic keratocysts (OKCs) by using Human Cytokine Antibody Array to identify the specific cytokines involved in formation and expansion of RCs and OKCs, respectively. There were significant differences in relative expression levels of IL-1 beta, MCP1, MIP1 beta, FGF-9, GDNF, HGF, IGFBP-3, Ang, IP-10, MIF, OPG, and TGF-beta2 between RC-CF and OKC-CF (P < .05). On the other hand, the cytokine expression patterns of RC-SUP (HGF, IL-8, NAP-2, IL-6, TIMP-1 and 2, GRO, IP-10, and Ang) were similar to those of OKC-SUP. Only the relative expression level of GRO differed between RC-SUP and OKC-SUP (P < .05). The similarities of cytokine production by tissue cultures derived from RC and OKC indicate that the expansion mechanisms of RC and OKC might involve similar biologic mechanisms other than infection.
authors.affiliation: Department of Endodontics, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hayashi
authors.forename: Takafumi
authors.initials: T
authors.lastname: Ohshima
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Ohshima
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Miyata
authors.forename: Osamu
authors.initials: O
authors.lastname: Takeichi
authors.forename: Bunnai
authors.initials: B
authors.lastname: Ogiso
authors.forename: Koichi
authors.initials: K
authors.lastname: Ito
authors.forename: Arne
authors.initials: A
authors.lastname: Ostman
authors.forename: Kichibee
authors.initials: K
authors.lastname: Otsuka
mesh_terms: D000328:Adult; D016207:Cytokines / Q000032:analysis* / Q000096:biosynthesis; D005260:Female; D006801:Humans; D008297:Male; D008336:Mandibular Diseases / Q000378:metabolism*; D008439:Maxillary Diseases / Q000378:metabolism*; D008875:Middle Aged; D009807:Odontogenic Cysts / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D011842:Radicular Cyst / Q000378:metabolism*; D018709:Statistics, Nonparametric
chemical_list: D016207:Cytokines; D012333:RNA, Messenger