title: [Trypanosoma rangeli parasite-vector-vertebrate interactions and their relationship to the systematics and epidemiology of American trypanosomiasis].
abstract: BACKGROUND
  Trypanosoma rangeli is a species of trypanosome second to T. cruzi, that is infective to humans in Latin America. Variability in the biological, biochemical and molecular characteristics between different isolates isolates of this parasite have been recorded.
  
  
  OBJECTIVE
  Morphological and molecular characteristics were recorded from strains of T. rangeli that were isolated from different species of Rhodnius and maintained in different vertebrate species.
  
  
  METHODS
  Nineteen strains of T. rangeli were isolated from R. prolixus, R. pallescens and R. colombiensis in Colombia, R. ecuadoriensis in Peru and R. pallescens in Panama. Polymorphism of blood trypomastigotes in ICR mice was evaluated and pleomorphism of P53 strain of T. rangeli KP1(-) inoculated in mouse, marsupial and canine was studied. RAPD analysis (randomly amplified polymorphic DNA analysis) of 12 strains isolated from four species of Rhodnius was performed.
  
  
  RESULTS
  Based on the total length of blood trypomastigotes, three discrete groups were observed. The P53 strain showed significant differences in the size of blood trypomastigotes in mouse, marsupial and canine. RAPD analysis showed that the strains segregated into two branches corresponding to strains of T. rangeli KP1(+) and T. rangeli KP1(-). All strains of T. rangeli KP1(-) clustered according to the species of Rhodnius from which they were isolated.
  
  
  CONCLUSIONS
  These data reveal, for the first time, a close association amongst T. rangeli strains and Rhodnius species, confirming that each species of Rhodnius transmits to vertebrate hosts a parasite population with clear phenotypic and genotypic differences. This is further evidence that supports the concept of clonal evolution of these parasites.
authors.affiliation: Laboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Universidad del Tolima, Ibagué, Colombia. gvallejo@ut.edu.co
authors.forename: Gustavo Adolfo
authors.initials: GA
authors.lastname: Vallejo
authors.forename: Felipe
authors.initials: F
authors.lastname: Guhl
authors.forename: Julio César
authors.initials: JC
authors.lastname: Carranza
authors.forename: Omar
authors.initials: O
authors.lastname: Triana
authors.forename: Gerardo
authors.initials: G
authors.lastname: Pérez
authors.forename: Paola Andrea
authors.initials: PA
authors.lastname: Ortiz
authors.forename: Dairo Humberto
authors.initials: DH
authors.lastname: Marín
authors.forename: Lina Marcela
authors.initials: LM
authors.lastname: Villa
authors.forename: Jazmín
authors.initials: J
authors.lastname: Suárez
authors.forename: Isaura Pilar
authors.initials: IP
authors.lastname: Sánchez
authors.forename: Ximena
authors.initials: X
authors.lastname: Pulido
authors.forename: Ingrid Bibiana
authors.initials: IB
authors.lastname: Rodríguez
authors.forename: Leyder Elena
authors.initials: LE
authors.lastname: Lozano
authors.forename: Daniel Alfonso
authors.initials: DA
authors.lastname: Urrea
authors.forename: Fredy Arvey
authors.initials: FA
authors.lastname: Rivera
authors.forename: César
authors.initials: C
authors.lastname: Cuba-Cuba
authors.forename: Jairo Alfonso
authors.initials: JA
authors.lastname: Clavijo
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000453:epidemiology / Q000469:parasitology / Q000635:transmission; D004285:Dogs; D006790:Host-Parasite Interactions; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology; D051379:Mice; D010802:Phylogeny; D012239:Rhodnius / Q000469:parasitology; D014345:Trypanosoma / Q000145:classification / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology
vernacular_title: Interacción tripanosoma-vector-vertebrado y su relación con la sistemática y la epidemiología de la tripanosomiasis Americana.