title: Evaluation of factors that can modify platelet-rich plasma properties.
abstract: OBJECTIVE
  The aim of this article is to discuss a protocol for obtaining platelet-rich plasma (PRP) and evaluate which factors, derived from its preparation method and from whole blood, modify PRP cytometry and coagulation time.
  
  
  METHODS
  Whole blood, harvested from 50 rabbits, was centrifuged at 300g for 10 minutes. Supernatant was recentrifuged at 5000g for 5 minutes. PRP was clotted with calcium chloride. Whole blood and PRP cytometry were obtained through automatic measurement. The amount of erythrocyte- and platelet-poor plasma drawn from whole blood was measured. Hematocrit, platelet and leukocyte count, mean corpuscular volume (MCV) and mean platelet volume (MPV), mean, standard deviation, and median were also calculated at whole blood and PRP. PRP coagulation time was also analyzed. Mean values between groups were analyzed using Student t test. Correlations were evaluated using Pearson's correlation coefficient. The significance level was set at P < .05. A linear regression was performed to investigate the relationship among the correlated variables.
  
  
  RESULTS
  From whole blood, 2.68 mL of erythrocytes and 5.72 mL of platelet-poor plasma (PPP) were removed. PRP platelet count was 2,324,080 cells/microL. Whole blood hematocrit influenced the amount of cells and PPP removed, as well as PRP platelet count. PRP platelet count was dependent on whole blood hematocrit and platelet count, and does not interfere in PRP coagulation time. A linear interaction was confirmed between the variables that presented significant Pearson correlation.
  
  
  CONCLUSIONS
  The protocol evaluated produces a good PRP. Whole-blood parameters can predict PRP features. Whole-blood hematocrit is an important variable for PRP preparation and PRP cytometry characterization. PRP platelet count is dependent upon whole-blood platelet count.
authors.affiliation: Bahia Foundation for Science Development and Postgraduate Program in Immunology, Bahia Federal University, Bahia, Brazil. miguelsetubal@hotmail.com
authors.forename: Miguel Gustavo Setúbal
authors.initials: MG
authors.lastname: Andrade
authors.forename: Claudio José
authors.initials: CJ
authors.lastname: de Freitas Brandão
authors.forename: Camila Neves
authors.initials: CN
authors.lastname: Sá
authors.forename: Thereza Cristina Borio Dos Santos Calmon
authors.initials: TC
authors.lastname: de Bittencourt
authors.forename: Moysés
authors.initials: M
authors.lastname: Sadigursky
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000166:cytology*; D004906:Erythrocyte Count; D004909:Erythrocyte Indices; D004912:Erythrocytes / Q000166:cytology*; D006400:Hematocrit; D007958:Leukocyte Count; D008297:Male; D010976:Platelet Count; D053657:Platelet-Rich Plasma / Q000737:chemistry / Q000166:cytology*; D011817:Rabbits