title: Differential proteomic analysis of HeLa cells treated with Honokiol using a quantitative proteomic strategy.
abstract: Honokiol (HNK) is an active component purified from Magnolia officinalis. HNK exhibits antitumor effects by inducing apoptosis and inhibiting the growth of many cancer cell lines, while proteins involved in antitumor effects in proteomic level are still unclear. In our study, HNK could inhibit HeLa cell proliferation and induce apoptosis in a concentration- and time-dependent manner. We utilized a quantitative proteomic technique termed SILAC (Stable isotope labeling with amino acids in cell culture)-MS (mass spectrometry) to study the differential proteomic profiling of HeLa cells treated by HNK. A total of 85 proteins were changed after HeLa cells were treated with 12 microg/ml HNK for 8 h, and 8 proteins showed up-regulation while 77 proteins down-regulated. The changed proteins were classified into 9 different categories, which covered a broad variety of cellular functions. In conclusion, HNK performs cytotoxicity to HeLa cells through co-operating of many proteins and different pathways.
authors.affiliation: West China Second Hospital, and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: B
authors.initials: B
authors.lastname: Ling
authors.forename: S-F
authors.initials: SF
authors.lastname: Liang
authors.forename: Y-H
authors.initials: YH
authors.lastname: Xu
authors.forename: X-Y
authors.initials: XY
authors.lastname: Zhao
authors.forename: M-H
authors.initials: MH
authors.lastname: Tang
authors.forename: X-Y
authors.initials: XY
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.forename: C-H
authors.initials: CH
authors.lastname: Huang
authors.forename: L-J
authors.initials: LJ
authors.lastname: Chen
authors.forename: Y-Q
authors.initials: YQ
authors.lastname: Wei
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001713:Biphenyl Compounds / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D003603:Cytotoxins / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000494:pharmacology*; D031566:Magnolia / Q000737:chemistry; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D013997:Time Factors; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001713:Biphenyl Compounds; D003603:Cytotoxins; D017705:Lignans; D009363:Neoplasm Proteins; D020543:Proteome; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols