title: Gas6 promotes inflammation by enhancing interactions between endothelial cells, platelets, and leukocytes.
abstract: The role of Gas6 in endothelial cell (EC) function remains incompletely characterized. Here we report that Gas6 amplifies EC activation in response to inflammatory stimuli in vitro. In vivo, Gas6 promotes and accelerates the sequestration of circulating platelets and leukocytes on activated endothelium as well as the formation and endothelial sequestration of circulating platelet-leukocyte conjugates. In addition, Gas6 promotes leukocyte extravasation, inflammation, and thrombosis in mouse models of inflammation (endotoxinemia, vasculitis, heart transplantation). Thus, Gas6 amplifies EC activation, thereby playing a key role in enhancing the interactions between ECs, platelets, and leukocytes during inflammation.
authors.affiliation: Department of Transgene Technology and Gene Therapy, Flanders Institute of Biotechnology (VIB), Leuven, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Tjwa
authors.forename: Lola
authors.initials: L
authors.lastname: Bellido-Martin
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Esther
authors.initials: E
authors.lastname: Lutgens
authors.forename: Stéphane
authors.initials: S
authors.lastname: Plaisance
authors.forename: Françoise
authors.initials: F
authors.lastname: Bono
authors.forename: Nathalie
authors.initials: N
authors.lastname: Delesque-Touchard
authors.forename: Caroline
authors.initials: C
authors.lastname: Hervé
authors.forename: Rute
authors.initials: R
authors.lastname: Moura
authors.forename: An D
authors.initials: AD
authors.lastname: Billiau
authors.forename: Cristina
authors.initials: C
authors.lastname: Aparicio
authors.forename: Marcel
authors.initials: M
authors.lastname: Levi
authors.forename: Mat
authors.initials: M
authors.lastname: Daemen
authors.forename: Mieke
authors.initials: M
authors.lastname: Dewerchin
authors.forename: Florea
authors.initials: F
authors.lastname: Lupu
authors.forename: Jef
authors.initials: J
authors.lastname: Arnout
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Herbert
authors.forename: Mark
authors.initials: M
authors.lastname: Waer
authors.forename: Pablo
authors.initials: P
authors.lastname: García de Frutos
authors.forename: Björn
authors.initials: B
authors.lastname: Dahlbäck
authors.forename: Peter
authors.initials: P
authors.lastname: Carmeliet
authors.forename: Marc F
authors.initials: MF
authors.lastname: Hoylaerts
authors.forename: Lieve
authors.initials: L
authors.lastname: Moons
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism / Q000473:pathology*; D002450:Cell Communication; D002460:Cell Line; D042783:Endothelial Cells / Q000201:enzymology / Q000473:pathology*; D004727:Endothelium / Q000378:metabolism; D016027:Heart Transplantation; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D017202:Myocardial Ischemia / Q000378:metabolism / Q000473:pathology; D015513:Oncogene Proteins / Q000378:metabolism; D019007:P-Selectin / Q000378:metabolism; D011518:Proto-Oncogene Proteins; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D014657:Vasculitis / Q000378:metabolism / Q000473:pathology; D000094963:Axl Receptor Tyrosine Kinase; D000099255:Growth Arrest-Specific Protein 6
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D019007:P-Selectin; D011518:Proto-Oncogene Proteins; D020794:Receptor Protein-Tyrosine Kinases; D000094963:Axl Receptor Tyrosine Kinase; D000099255:Growth Arrest-Specific Protein 6