title: Severe intrauterine growth restriction pregnancies have increased placental endoglin levels: hypoxic regulation via transforming growth factor-beta 3.
abstract: Endoglin, a co-receptor for transforming growth factor (TGF)-beta 1 and -beta 3 is expressed in the human placenta and plays an important role in the pathogenesis of preeclampsia. Because preeclampsia is associated with hypoxia, and because TGF-beta 3 is overexpressed in preeclamptic pregnancies, we examined the effect of oxygen and TGF-beta 3 on placental endoglin expression and investigated its expression in pathological models of placental hypoxia such as intrauterine growth restriction (IUGR) pregnancies. Endoglin expression was high at 4 to 9 weeks of gestation, when oxygen tension is low, and decreased after 10 weeks, when oxygen tension increases. Exposure of villous explants to low oxygen (3% O2) resulted in elevated expression of both membrane and soluble endoglin compared to standard conditions (20% O2). Moreover, addition of TGF-beta 3 to villous explants under low oxygen conditions increased the expression of endoglin compared to nontreated explants whereas addition of TGF-beta 3-neutralizing antibodies inhibited the low oxygen stimulatory effect on endoglin expression. Endoglin and soluble endoglin expression were significantly increased in placentas of IUGR singletons compared to controls and in the IUGR twin placentas relative to both the control co-twin and the normal twins. These data demonstrate that oxygen regulates the placental expression of endoglin via TGF-beta 3. Reduced placental perfusion leading to placental hypoxia might contribute to the increased expression of endoglin in IUGR pregnancies.
authors.affiliation: Department of Obstetrics and Gynecology, Mount Sinai Hospital, Samuel Lunenfeld Research Institute, 600 University Ave., Room 871c, Toronto, Ontario, Canada M5G 1X5.
authors.forename: Yoav
authors.initials: Y
authors.lastname: Yinon
authors.forename: Ori
authors.initials: O
authors.lastname: Nevo
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Ariel
authors.initials: A
authors.lastname: Many
authors.forename: Alessandro
authors.initials: A
authors.lastname: Rolfo
authors.forename: Tullia
authors.initials: T
authors.lastname: Todros
authors.forename: Martin
authors.initials: M
authors.lastname: Post
authors.forename: Isabella
authors.initials: I
authors.lastname: Caniggia
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis*; D004200:Diseases in Twins; D000071063:Endoglin; D005260:Female; D005317:Fetal Growth Retardation / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D000860:Hypoxia; D007150:Immunohistochemistry; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D013997:Time Factors; D053782:Transforming Growth Factor beta3 / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface; D053782:Transforming Growth Factor beta3; D010100:Oxygen