title: Heterogeneous rRNA molecules encoded by Streptomyces coelicolor M145 genome are all expressed and assembled into ribosomes.
abstract: The Streptomyces coelicolor M145 genome harbors six copies of divergent rRNA operons that differ at ~0.2% and ~0.6% of the nucleotide positions in small subunit (SSU) and large subunit (LSU) rRNA genes, respectively. When these rRNA genes are expressed, a single cell may harbor three different kinds of SSU rRNA and five kinds of LSU rRNA. Primer extension analyses revealed that all of the heterogeneous rRNA molecules are expressed and assembled into ribosomes. This finding and the maintenance of the intragenomic variability of rRNA operons imply the existence of functional divergence of rRNA species in this developmentally complex microorganism.
authors.affiliation: Graduate School of Life Science and Biotechnology, Pochon CHA University, Seongnam, Korea.
authors.forename: Hyun-Lee
authors.initials: HL
authors.lastname: Kim
authors.forename: Eunkyoung
authors.initials: E
authors.lastname: Shin
authors.forename: Hong-Man
authors.initials: HM
authors.lastname: Kim
authors.forename: Hayoung
authors.initials: H
authors.lastname: Go
authors.forename: Jaesook
authors.initials: J
authors.lastname: Roh
authors.forename: Jeehyeon
authors.initials: J
authors.lastname: Bae
authors.forename: Kangseok
authors.initials: K
authors.lastname: Lee
mesh_terms: D014644:Genetic Variation; D016680:Genome, Bacterial; D009876:Operon; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000235:genetics* / Q000378:metabolism; D054681:Ribosome Subunits, Large, Bacterial / Q000235:genetics; D054680:Ribosome Subunits, Small, Bacterial / Q000235:genetics; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000378:metabolism
chemical_list: D012329:RNA, Bacterial; D012335:RNA, Ribosomal