title: Serum proteomic-based analysis for the identification of a potential serological marker for autoimmune hepatitis.
abstract: In the present study, by using a serologic proteomic analysis, we identified phosphoglycerate mutase isozyme B (PGAM-B) as a putative target of autoantibodies in autoimmune hepatitis (AIH). To evaluate whether the identified autoantigen is crucial for AIH, we cloned PGAM-B cDNA and expressed the recombinant protein in Escherichia coli. The soluble PGAM-B was purified by affinity chromatography and used as a coating antigen to determine the frequency of the PGAM-B-autoantibodies (PGAM-B-Abs) in patients with AIH and primary biliary cirrhosis (PBC) as well as chronic hepatitis B (CHB), chronic hepatitis C (CHC), and healthy donors by ELISA. Our study showed that the autoantibody to PGAM-B was predominantly present in AIH patients and 70.04% (50/71) of the tested AIH sera reacted to PGAM-B. The frequency of autoantibodies to PGAM-B is much higher in patients with AIH than in patients with PBC, CHB, CHC, and normal control. The data were further confirmed by using 1-DE Western blot analysis. Our study presents the first description of this protein as a candidate of diagnostic marker for AIH.
authors.affiliation: School of Preclinical and Forensic Medicine, West China Medical Center of Sichuan University, Chengdu 610041, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Lu
authors.forename: Qing
authors.initials: Q
authors.lastname: Xia
authors.forename: Yuanfang
authors.initials: Y
authors.lastname: Ma
authors.forename: Guogang
authors.initials: G
authors.lastname: Yuan
authors.forename: Huiping
authors.initials: H
authors.lastname: Yan
authors.forename: Lu
authors.initials: L
authors.lastname: Qian
authors.forename: Meiru
authors.initials: M
authors.lastname: Hu
authors.forename: Mingli
authors.initials: M
authors.lastname: Wang
authors.forename: Han
authors.initials: H
authors.lastname: Lu
authors.forename: Hongli
authors.initials: H
authors.lastname: Wang
authors.forename: Bingyu
authors.initials: B
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Xue
authors.forename: Hongxia
authors.initials: H
authors.lastname: Wang
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Li
authors.forename: Beifen
authors.initials: B
authors.lastname: Shen
authors.forename: Ning
authors.initials: N
authors.lastname: Guo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D005240:Feasibility Studies; D005260:Female; D019693:Hepatitis, Autoimmune / Q000097:blood* / Q000175:diagnosis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010736:Phosphoglycerate Mutase / Q000097:blood*; D020543:Proteome / Q000032:analysis*
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D020543:Proteome; D010736:Phosphoglycerate Mutase