Fluorescent proteins for photoactivation experiments.
The discoveries, improvements, and alterations of fluorescent protein (FP) variants are having a profound impact on the ability of investigators to observe and quantify the behavior of proteins within cells and organisms. Among the most promising of FPs are photoactivatable fluorescent proteins (PA-FPs). Invisible at the imaging wavelength until activated by irradiation at a different wavelength, PA-FPs allow the controlled highlighting of distinct molecular populations within the cell. This chapter introduces the different types of PA-FPs and discusses their use for monitoring protein movement, protein turnover, protein interactions, and high-resolution protein localization.
D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D049452:Green Fluorescent Proteins; D008027:Light; D008163:Luminescent Measurements; D008164:Luminescent Proteins; D008856:Microscopy, Fluorescence / Q000379:methods; D038761:Photobleaching; D013050:Spectrometry, Fluorescence
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins