title: PCR-mediated deletion of plasmid DNA.
abstract: The PCR-mediated plasmid DNA deletion method is a simple approach to delete DNA sequences from plasmids using only one round of PCR, with two primers, and without ligation or purification prior to in vivo recombination. By using only PCR, the method is sequence independent and, as shown in this study, is applicable to various sizes of plasmids and deletions using minimal primer design.
authors.affiliation: Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, S-22100 Lund, Sweden. mattias.hansson@biochemistry.lu.se
authors.forename: Mattias D
authors.initials: MD
authors.lastname: Hansson
authors.forename: Kamila
authors.initials: K
authors.lastname: Rzeznicka
authors.forename: Matilda
authors.initials: M
authors.lastname: Rosenbäck
authors.forename: Mats
authors.initials: M
authors.lastname: Hansson
authors.forename: Nick
authors.initials: N
authors.lastname: Sirijovski
mesh_terms: D004247:DNA / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017384:Sequence Deletion / Q000235:genetics*
chemical_list: D004247:DNA