title: Trypanosoma evansi: molecular homogeneity as inferred by phenetical analysis of ribosomal internal transcribed spacers DNA of an eclectic parasite.
abstract: The protozoan Trypanosoma evansi is described as presenting high morphological and genetic similarities among the isolates despite its biological heterogeneity and wide geographical distribution. PCR amplification of the internal transcribed spacers of the ribosomal gene in combination with the coding region of the 5.8S ribosomal subunit further submitted to restriction enzymes digestion were carried out in DNAs extracted from 41 T. evansi strains isolated from horses, dogs, coatis and capybaras from two distinct regions of the Brazilian Pantanal. We also used one T. evansi isolate from Africa, one from Asia and one isolate of T. b. brucei from Africa. Analysis of the RFLP profiles yielded a unique "riboprinting" that does not vary intraspecifically. These results provide insights on the ribosomal gene organization of T. evansi and showed that ITS analysis by RFLP show high genetic similarity of this locus among isolates of this protozoan parasite.
authors.affiliation: Laboratory of Tripanosomatid Biology, Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil 4365, CEP. 21040-360, Rio de Janeiro/RJ, Brazil.
authors.forename: Aneska Norek
authors.initials: AN
authors.lastname: de Oliveira Lima
authors.forename: Simone
authors.initials: S
authors.lastname: da Silva Santos
authors.forename: Heitor Miraglia
authors.initials: HM
authors.lastname: Herrera
authors.forename: Carla
authors.initials: C
authors.lastname: Gama
authors.forename: Elisa
authors.initials: E
authors.lastname: Cupolillo
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Jansen
authors.forename: Octávio
authors.initials: O
authors.lastname: Fernandes
mesh_terms: D000818:Animals; D001938:Brazil; D002162:Camelus; D002417:Cattle; D002681:China; D016054:DNA, Protozoan / Q000737:chemistry; D021903:DNA, Ribosomal Spacer / Q000737:chemistry*; D004285:Dogs; D005002:Ethiopia; D006736:Horses; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D045988:Procyonidae; D012377:Rodentia; D012756:Sheep; D014345:Trypanosoma / Q000145:classification / Q000235:genetics*; D014346:Trypanosoma brucei brucei / Q000145:classification / Q000235:genetics; D014454:Uganda
chemical_list: D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer