[Immunohistochemical assay of cellular cycle markers: an alternative to chip-diagnosis of breast cancer].
A new method of evaluation of immunohistochemical markers of cellular cycle (K-67, topoisomerase-II-alpha, P21/wafl), adhesion molecules (E-cadherin, CD33v6), oncoprotein HER-2, and estrogen and progesterone receptors of tumor is presented. High-precision count of tumor cells, which express each marker, was carried out using serial paraffin sections and Leica CTR5000 morphometric station and Leica Quin Plus program, to identify tumor sensitivity to anthracyclines and taxanes. Proliferative potential, tumor sensitivity to key chemical drugs and prognosis were evaluated on the basis of the evidence obtained and, in particular, the role of the proteins under study played in cellular cycle.
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D015820:Cadherins / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis*; D018797:Cell Cycle Proteins / Q000032:analysis*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000032:analysis; D004250:DNA Topoisomerases, Type II / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008875:Middle Aged; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011379:Prognosis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis*; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*; D063268:Sialic Acid Binding Ig-like Lectin 3
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015820:Cadherins; D015815:Cell Adhesion Molecules; D018797:Cell Cycle Proteins; D050759:Cyclin-Dependent Kinase Inhibitor p21; D004250:DNA Topoisomerases, Type II; D004268:DNA-Binding Proteins; D019394:Ki-67 Antigen; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D063268:Sialic Acid Binding Ig-like Lectin 3; C571163:CD33 protein, human; C493956:CDKN1A protein, human