Determination of glycosyltransferase specificities for the Escherichia coli O111 O antigen by a generic approach.
We describe a bacterial strain developed to facilitate the determination of glycosyltransferase (GT) specificities for O antigens of known structure and gene cluster sequence. For proof of principle for the approach, the strain was used to determine the specificity of the Escherichia coli O111 O-antigen GT genes.
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D005810:Multigene Family; D019081:O Antigens / Q000235:genetics / Q000378:metabolism*; D009841:Oligonucleotides / Q000235:genetics; D013045:Species Specificity; D013379:Substrate Specificity
D019081:O Antigens; D009841:Oligonucleotides; D005964:Glucosyltransferases