title: A family of eukaryotic lysophospholipid acyltransferases with broad specificity.
abstract: The budding yeast ALE1 gene encodes a lysophospholipid acyltransferase (LPLAT) with broad specificity. We show that yeast LPLAT (ScLPLAT) belongs to a distinct protein family that includes human MBOAT1, MBOAT2, MBOAT4, and several closely related proteins from other eukaryotes. We further show that two plant proteins within this family, the Arabidopsis proteins AtLPLAT1 and AtLPLAT2, possess lysophospholipid acyltransferase activities similar to ScLPLAT. We propose that other members of this protein family, which we refer to as the LPLAT family, also are likely to possess LPLAT activity. Finally, we show that ScLPLAT differs from the specific lysophosphatidic acid acyltransferase that is encoded by SLC1 in that it cannot efficiently use lysophosphatidic acid produced by acylation of glycerol-3-phosphate in vitro.
authors.affiliation: Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden. ulf.stahl@vbsg.slu.se
authors.forename: Ulf
authors.initials: U
authors.lastname: Ståhl
authors.forename: Kjell
authors.initials: K
authors.lastname: Stålberg
authors.forename: Sten
authors.initials: S
authors.lastname: Stymne
authors.forename: Hans
authors.initials: H
authors.lastname: Ronne
mesh_terms: D008243:1-Acylglycerophosphocholine O-Acyltransferase / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D010802:Phylogeny; D013379:Substrate Specificity
chemical_list: D017931:DNA Primers; D008243:1-Acylglycerophosphocholine O-Acyltransferase