title: [Real-time measurement of noradrenaline release in central nervous system].
abstract: In order to investigate the central nervous mechanism and the diseases involved in catecholamine transmitter secretion, the dynamics of catecholamine release is studied in single cell, brain slice or in vivo. Noradrenaline is an important neurotransmitter and modulator in the central nervous system (CNS) and the peripheral nervous system (PNS). In the present paper, we first compared three real-time methods used to measure noradrenaline secretion in single cells (membrane capacitance, amperometry and confocal fluorescence microscopy imaging). Compared to the electrophysiological method and fluorescence microscopy, the basic usage of the carbon fiber electrode (CFE) in neuroscience research was presented as an example. Then, we presented a primary description of ion channels, including voltage-gated Na(+), K(+) and Ca(2+) channels in locus coeruleus (LC) neurons in rat brain slices. Finally, we presented example recordings of combined patch-clamp and amperometry measurements in LC neurons, indicating Ca(2+)-dependent quantal noradrenaline release following Ca(2+) influx through Ca(2+) channels.
authors.affiliation: College of Basic Medicine, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Hong-Ping
authors.initials: HP
authors.lastname: Huang
authors.forename: Yu
authors.initials: Y
authors.lastname: Mu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Mu
authors.initials: M
authors.lastname: Jin
authors.forename: Jing
authors.initials: J
authors.lastname: Lv
authors.forename: Jing-Li
authors.initials: JL
authors.lastname: Gu
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiu
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Ning
authors.initials: N
authors.lastname: Guo
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.forename: Mei-Ying
authors.initials: MY
authors.lastname: Song
authors.forename: Claire Xi
authors.initials: CX
authors.lastname: Zhang
authors.forename: Huai-Zhen
authors.initials: HZ
authors.lastname: Ruan
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000502:physiology*; D007473:Ion Channels / Q000502:physiology; D009638:Norepinephrine / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats
chemical_list: D007473:Ion Channels; D009638:Norepinephrine