title: J-based 2D homonuclear and heteronuclear correlation in solid-state proteins.
abstract: Scalar-based two-dimensional heteronuclear experiments are reported for NCO and NCA chemical shift correlation in the solid state. In conjunction with homonuclear CACO correlation, these experiments form a useful set for tracing connectivities and assigning backbone resonances in solid-state proteins. The applicability of this approach is demonstrated on two proteins, the β 1 immunoglobulin binding domain of protein G at 9.4 T and reassembled thioredoxin at 14.1 T, using different decoupling conditions and MAS frequencies. These constant-time J-based correlation experiments exhibit increased resolution in the indirect dimension owing to homonuclear and heteronuclear decoupling, and because the indirect evolution and transfer periods are combined into a single constant time interval, this increased resolution is not obtained at the cost of sensitivity. These experiments are also shown to be compatible with in-phase anti-phase (IPAP) selection, giving increased resolution in the directly detected dimension.
authors.affiliation: Department of Chemistry, University of California, Riverside, California 92521, USA.
authors.forename: Lingling
authors.initials: L
authors.lastname: Chen
authors.forename: J Michael
authors.initials: JM
authors.lastname: Kaiser
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Lai
authors.forename: Tatyana
authors.initials: T
authors.lastname: Polenova
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Chad M
authors.initials: CM
authors.lastname: Rienstra
authors.forename: Leonard J
authors.initials: LJ
authors.lastname: Mueller
mesh_terms: D000465:Algorithms; D001426:Bacterial Proteins / Q000737:chemistry*; D002247:Carbon Isotopes; D007136:Immunoglobulins / Q000737:chemistry*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D013879:Thioredoxins / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D002247:Carbon Isotopes; C034284:IgG Fc-binding protein, Streptococcus; D007136:Immunoglobulins; D009587:Nitrogen Isotopes; D011994:Recombinant Proteins; D013879:Thioredoxins