[Molecular genetic monitoring of Yersinia pseudotuberculosis using PCR O-genotyping].
Irkutsk PCR O-genotyping of 117 Yersinia pseudotuberculosis serotype O:1-O:4 strains isolated in Siberia and Far East was performed. These methods allowed both O-genotype and its variants (a, b, c) to be detected. It was demonstrated that three Y. pseudotuberculosis O-genotypes were circulated in Siberia (O:1a, O:1b and 0:3) and six genotypes (O:1a, O:1b, O:1c, O:2a, O:3 and O:4b) were circulated at Far East. Genotype O:1b dominates at both regions (87.8%). PCR-algorithm for identification of Y. pseudotuberculosis O-genotypes having epidemic significance was developed. The identification of the etiological agent O-genotype without bacteriological isolation of the stimulus is possible by PCR analysis of the clinical material.
D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods*; D005838:Genotype; D006801:Humans; D019081:O Antigens / Q000235:genetics; D016133:Polymerase Chain Reaction; D012703:Serotyping / Q000379:methods*; D015011:Yersinia pseudotuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015012:Yersinia pseudotuberculosis Infections / Q000382:microbiology*
D004269:DNA, Bacterial; D019081:O Antigens