title: Photodynamic antimicrobial chemotherapy (PACT) with methylene blue increases membrane permeability in Candida albicans.
abstract: Photodynamic antimicrobial chemotherapy (PACT) is a potential antimicrobial therapy that combines light and a photosensitizing drug, promoting a phototoxic effect on the treated cells, in general via oxidative damage. In this work we studied the effect of PACT, using methylene blue (MB), on the permeability of Candida albicans membrane. Our results demonstrated that the combination of MB and laser (684 nm) promoted a decrease in Candida growth. The inhibition was more pronounced in the presence of 0.05 mg/ml MB and with an energy density of 28 J/cm(2). The decrease in Candida growth was associated with an increase in membrane permeabilization. Thus, we suggest that a PACT mechanism using MB can be related to damage in the plasma membranes of the cells.
authors.affiliation: Instituto de Pesquisa e Desenvolvimento (IP&D), Universidade do Vale do Paraíba (UNIVAP), São José dos Campos, SP, Brazil.
authors.forename: Ligia Maria
authors.initials: LM
authors.lastname: Giroldo
authors.forename: Monalisa Poliana
authors.initials: MP
authors.lastname: Felipe
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: de Oliveira
authors.forename: Egberto
authors.initials: E
authors.lastname: Munin
authors.forename: Leandro Procópio
authors.initials: LP
authors.lastname: Alves
authors.forename: Maricilia Silva
authors.initials: MS
authors.lastname: Costa
mesh_terms: D002176:Candida albicans / Q000187:drug effects* / Q000528:radiation effects*; D002463:Cell Membrane Permeability / Q000187:drug effects* / Q000528:radiation effects*; D005470:Fluorometry; D007834:Lasers; D008751:Methylene Blue / Q000494:pharmacology*; D010778:Photochemotherapy
chemical_list: D008751:Methylene Blue