title: Cloning and expression of the tubulin genes in barley.
abstract: Tubulins are encoded by small gene families in plants. Based on the barley EST collection, cDNAs for alpha-, beta-, and gamma-tubulins were selected. Five genes for alpha-tubulin, eight newly identified beta-tubulin sequences and one gamma-tubulin gene were found to be expressed in barley. In silico analysis of relative abundance of the distinct tubulin sequences among ESTs derived from different libraries revealed that the various tubulin genes differed in their level of expression, and to some extent were tissue specific.
authors.affiliation: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466 Gatersleben, Germany. radchukv@ipk-gatersleben.deradchukv@ipk-gatersleben.de
authors.forename: Volodymyr
authors.initials: V
authors.lastname: Radchuk
authors.forename: Nese
authors.initials: N
authors.lastname: Sreenivasulu
authors.forename: Yaroslav
authors.initials: Y
authors.lastname: Blume
authors.forename: Winfriede
authors.initials: W
authors.lastname: Weschke
mesh_terms: D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics*; D001467:Hordeum / Q000235:genetics*; D009928:Organ Specificity; D014404:Tubulin / Q000235:genetics*
chemical_list: D014404:Tubulin