title: Analysis on the origin and phylogenetic status of Tong sheep using 12 blood protein and nonprotein markers.
abstract: This study is based on the Tong sheep obtained by the random sampling method of typical colonies in the central area of Baishui County in Shaanxi Province, China. An investigation was undertaken to clarify the gene constitution of blood protein and nonprotein types of Tong sheep. Twelve genetic markers were examined by starch-gel electrophoresis and cellulose acetate electrophoresis. Polymorphism in Tong sheep was found at the following 10 loci, transferrin (Tf), alkaline phosphatase (Alp), leucine aminopeptidase (Lap), arylesterase (Ary-Es), hemoglobin-beta (Hb-beta), X-protein (X-p), carbonic anhydrase (CA), catalase (Cat), malate dehydrogenase (MDH), and lysine (Ly), whereas, albumin (Al) and postalbumin (Po) loci were monomorphic. Genetic approach degree method and phylogenetic relationship clustering method were used to judge the origin and phylogenetic status of Tong sheep. Results from both methods maintained that Tong sheep belonged to the "Mongolia group", and Mongolia sheep was the origin of Tong sheep. This was also supported by the history of Tong sheep breeding. Compared to the phylogenetic relationship clustering method, the genetic approach degree method was more reliable for the extraction from East and South of Central Asia, and was more effective in reflecting the breeding course of Tong sheep.
authors.affiliation: Animal Science & Technology College, Yangzhou University, Yangzhou 25009, China. dkxsunwei@163.com
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Hong
authors.initials: H
authors.lastname: Chang
authors.forename: Zhangping
authors.initials: Z
authors.lastname: Yang
authors.forename: Rongqing
authors.initials: R
authors.lastname: Geng
authors.forename: Kenji
authors.initials: K
authors.lastname: Tsunoda
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Ren
authors.forename: Hongyu
authors.initials: H
authors.lastname: Chen
authors.forename: Musa H
authors.initials: MH
authors.lastname: Hussein
mesh_terms: D000818:Animals; D001769:Blood / Q000378:metabolism*; D001798:Blood Proteins / Q000235:genetics*; D016000:Cluster Analysis; D019143:Evolution, Molecular; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics; D010802:Phylogeny; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012756:Sheep / Q000097:blood / Q000145:classification* / Q000235:genetics*
chemical_list: D001798:Blood Proteins; D005819:Genetic Markers