title: Highly sensitive assays for SUMOylation and small ubiquitin-like modifier-dependent protein-protein interactions.
abstract: Small ubiquitin-like proteins (SUMO) are recently discovered post-translational modifiers that regulate protein functions and intracellular trafficking. In this study, we are describing two chemoluminescence-based assays, one for SUMOylation and another one for SUMO-mediated protein-protein interactions. These assays can be used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation, and in high-throughput screening for inhibitors of SUMOylation and SUMO-dependent protein-protein interactions. These novel assays represent the most sensitive assays for ubiquitin-like systems published to date. Similar strategies can be used to develop assays for other ubiquitin-like modification systems.
authors.affiliation: PerkinElmer BioSignal, Montreal, Quebec H3J 1R4, Canada. nathalie.rouleau@perkinelmer.com
authors.forename: Nathalie
authors.initials: N
authors.lastname: Rouleau
authors.forename: Jianghai
authors.initials: J
authors.lastname: Wang
authors.forename: Labrini
authors.initials: L
authors.lastname: Karras
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Andrews
authors.forename: Martina
authors.initials: M
authors.lastname: Bielefeld-Sevigny
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D007700:Kinetics; D008163:Luminescent Measurements; D011485:Protein Binding; D012680:Sensitivity and Specificity; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D025841:Small Ubiquitin-Related Modifier Proteins; D025801:Ubiquitin