title: Preliminary study of the anti-inflammatory activity of hexane extract and fractions from Bursera simaruba (Linneo) Sarg. (Burseraceae) leaves.
abstract: Bursera simaruba (L.) Sarg. leaves hexane extracts display anti-inflammatory activity on the adjuvant-carrageenan-induced inflammation in rats. In order to isolate and identify the active compounds of the hexane extract, we performed a preliminary phytochemical study and a bioassay-directed fractionation using the carrageenan-induced paw oedema test in mice. From the nine fractions (A-I) obtained, of an initial chromatographic separation, fractions A and E (doses equivalents to 1.50 g dry plant/kg body weight) showed the strongest anti-inflammatory activity comparable to that of the reference drug phenylbutazone (80 mg/kg). The isolation and characterization of 3-methylene-7,11,15-trimethylhexadec-1-ene (neophytadiene) (1), ergost-5-en-3beta-ol (2), 24S-stigmast-5,22E-dien-3beta-ol (3), 24S-stigmast-5-en-3beta-ol (4) and alpha-amyrin (5), from these fractions is reported.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Universidad Complutense de Madrid, 28040 Madrid, Spain. meca@harm.ucm.es
authors.forename: M E
authors.initials: ME
authors.lastname: Carretero
authors.forename: J L
authors.initials: JL
authors.lastname: López-Pérez
authors.forename: M J
authors.initials: MJ
authors.lastname: Abad
authors.forename: P
authors.initials: P
authors.lastname: Bermejo
authors.forename: S
authors.initials: S
authors.lastname: Tillet
authors.forename: A
authors.initials: A
authors.lastname: Israel
authors.forename: B
authors.initials: B
authors.lastname: Noguera-P
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D031233:Bursera / Q000737:chemistry*; D005260:Female; D051379:Mice; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D013755:Tetradecanoylphorbol Acetate